White sturgeon (Acipenser transmontanus) passage at the Dalles Dam, Columbia River, USA
White sturgeon (Acipenser transmontanus) ???95 cm TL were monitored using acoustic and radio telemetry at a large hydroelectric dam (the Dalles Dam) on the Columbia River, during March 2004 through November 2005 to determine timing and routes of passage and to characterize general movements. Transmitters were surgically implanted into 148 fish during the study; 90 were released into the tailrace and 58 into the forebay. We documented 26 passage events by 19 tagged fish: eight upstream via fish ladders and 18 downstream, mostly through open spill gates. During the study 17 fish entered the two ladders one or more times; 11 entered only the east ladder, three entered only the north ladder, and three entered both ladders at sometime. Residence time within the ladders by individual fish was variable, ranging from about 1 min to nearly 6 months (median = 7.7 h). Only six fish successfully ascended the east ladder, one fish twice. We could not unequivocally determine which fish ladder one fish used to pass upstream. Differences in construction between the north and east fish ladders may account for the greater success of the east fish ladder in passing sturgeon upstream. Changes to operations at hydroelectric dams to benefit migrating anadromous salmonids may influence upstream or downstream passage by white sturgeon. Altering patterns and timing of spill discharge, altering fish ladder entrance attraction flows, and the use of lights, sound, and partial barriers to direct other species of fish to preferred passage routes have unknown effects on sturgeon passage. A better understanding of the consequences to the metapopulation of increasing or precluding upstream or downstream passage is needed. ?? 2007 The Authors.
Abstract in portuguese A seleção específica de uma escada de peixes do tipo seqüência de tanques, com passagem de fundo (tipo weir and orifice), na barragem da hidrelétrica de Itaipu (rio Paraná), foi avaliada através de amostragens no rio a jusante (amostragem trimestral em 1997) e em dois pontos ao longo da escada (10 e 27m de altura; nov/94 a jan/97 e nov/94 a fev/97, respectivamente). Das 65 espécies registradas no rio imediatamente a jusante da barragem, 27 foram capturadas na esc (more) ada. As espécies com maior densidade na escada, em sua maioria migradoras, tiveram abundância moderada ou baixa a jusante. Entre as mais abundantes a jusante, apenas uma, não migradora, foi registrada na escada. A escada apresentou seleção negativa aos grandes pimelodídeos migradores que pode ser superada com a ampliação na escala do projeto. As amostragens evidenciaram baixa seletividade específica ao longo da escada, sendo seu modelo hidráulico satisfatório na atração e eficiente na ascensão de peixes. Abstract in english The specific selection of a weir and orifice type experimental fish ladder in the dam of the Itaipu Reservoir (Paraná River) was evaluated by samplings in the river downstream and at two points along the ladder (at heights of 10 m and 27 m) during 28 months. Among the 65 species recorded in the river (immediate downstream of the dam), 27 were captured on the ladder. The species that showed highest density on the ladder, the majority migratory, were moderately, or only sl (more) ightly, abundant downstream. Among the most abundant species downstream, only one, non-migratory, was recorded in the ladder. The structure presented a negative selection in relation to large migratory pimelodids that might be overcome by enlarging the scale of its design. The sampling demonstrated a moderate selection of species along the ladder and its hydraulic model proved satisfactory regarding the attraction and efficient ascent of the fishes.
Walla Walla River Fish Passage Operations Program, 2004-2005 Annual Report.
In the late 1990s, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate fish migration conditions in the basin. The migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow requirements, and providing trap and haul efforts when needed. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survival of migrating adult and juvenile salmonids in the Walla Walla River basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage criteria and passage and trapping facility design and operation. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. During the 2004-2005 project year, there were 590 adult summer steelhead, 31 summer steelhead kelts (Oncorhynchus mykiss), 70 adult bull trout (Salvelinus confluentus); 80 adult and 1 jack spring Chinook (O. tshawytscha) enumerated at the Nursery Bridge Dam fishway video counting window between December 13, 2004, and June 16, 2005. Summer steelhead and spring chinook were observed moving upstream while bull trout were observed moving both upstream and downstream of the facility. In addition, the old ladder trap was operated by ODFW in order to enumerate fish passage. Of the total, 143 adult summer steelhead and 15 summer steelhead kelts were enumerated at the west ladder at Nursery Bridge Dam during the video efforts between February 4 and May 23, 2005. Operation of the Little Walla Walla River juvenile trap for trap and haul purposes was not necessary this year.
Sediment Transport through Road Culverts Retrofit for Fish Passage
A critical component of watershed restoration includes improved mobility within the watershed for fish and other aquatic organisms. At the large scale, this effort includes installation of fish ladders around dams and at smaller scales replacement or retrofit of road culverts or installation of roughened channels to mitigate steep channel slopes. A project to quantify changes in culvert hydraulic performance and hydraulic capacity for three culvert shapes and six fish passage retrofit designs was initiated in June 2005. In 2007, laboratory physical model experiments were conducted to evaluate sediment transport and trapping characteristics of these designs over a range of flows. Generally, experimental results indicate trapped sediment in culverts retrofit to improve fish passage decreases the effectiveness of the retrofit due to sediment deposition in areas with lower velocities (where fish can rest). Other observations include: 1. Trapped sediment reduced the effective culvert barrel roughness and, thus, decreased water depths and increased velocities through the culvert, compared to clear water experiments with the retrofit baffles. 2. High flows (culvert barrel water depth/culvert height greater than 0.5) successfully cleared trapped sediment under conditions of minimal transport from upstream 3. Preliminary results indicate moderate flows (culvert barrel water depth/culvert height between 0.25 to 0.5) in combination with moderate sediment feed rates caused the greatest accumulation of trapped sediment These experiments highlight the importance of including sediment accumulation in design and analysis, and potentially impact design recommendations for culverts retrofit for fish passage and other similar fish passage improvement structures.
We report on our progress from October 1991 through September 1992 in evaluating juvenile fish bypass facilities at Three Mile Falls and Westland dams on the Umatilla River. We also report on our progress from October 1991 through June 1992 in evaluating adult fish passage in the lower Umatilla River and adult fish passage facilities at Three Mile Falls Dam. The study is a cooperative effort by the Oregon Department of Fish and Wildlife (ODFW) and the Confederated Tribes of the Umatilla Indian Reservation (CTUIR). These are the study objectives addressed by ODFW and CTUIR: (1) Report A (ODFW): To evaluate the juvenile fish bypass facility in the West Extension Irrigation District Canal at Three Mile Falls Dam and document juvenile salmonid passage through the juvenile fish bypass facility and east-bank adult fish ladder. To measure velocity and develop trap designs at Westland Dam. (2) Report B (CTUIR): To examine the passage of adult salmonids at Three Mile Falls Dam. The study is part of a program to rehabilitate anadromous fish stocks in the Umatilla River Basin, including restoration of coho salmon (Oncorhynchus kisutch) and chinook salmon (Oncorhynchus tshawytscha), as well as enhancement of summer steelhead (Oncorhynchus mykiss).
Fish ladders select fish traits on migration-still a growing problem for natural fish populations
We investigated the potential selective effect of fish ladders on physiological and morphological profiles of the curimbata, Prochilodus lineatus, during reproductive migration in Brazil. We registered sex, body weight and length, plasma glucose, hepatosomatic and gonadosomatic indices (HSI and GSI, respectively), hematocrit, leucocrit, blood cell and nucleus areas, and the diameter of white and red muscle fibers in fish sampled at the bottom (downstream) and at the top (upstream) of a fish ladder at a hydroelectric dam. Males and females at the top of the ladder showed higher size (weight and length), white muscle fiber diameters, plasma glucose levels and lower hematocrit when compared with those at the bottom. These size and muscle traits assist fish to overcome the ladder barrier and b...
In the late 1990s, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate fish migration conditions in the basin. Migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow requirements, and providing trap and haul efforts when needed. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survival of migrating adult and juvenile salmonids in the Walla Walla River basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage and trapping facility design, operation, and criteria. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. Beginning in March of 2007, two work elements from the Walla Walla Fish Passage Operations Project were transferred to other projects. The work element Enumeration of Adult Migration at Nursery Bridge Dam is now conducted under the Walla Walla Basin Natural Production Monitoring and Evaluation Project and the work element Provide Transportation Assistance is conducted under the Umatilla Satellite Facilities Operation and Maintenance Project. Details of these activities can be found in those project's respective annual reports.
Walla Walla River Fish Passage Operations Program, 2003-2004 Annual Report.
In the late 1990s, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate fish migration conditions in the basin. The migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow requirements, and providing trap and haul efforts when needed. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survival of migrating adult and juvenile salmonids in the Walla Walla River basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage criteria and passage and trapping facility design and operation. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. During the 2003-2004 project year, there were 379 adult summer steelhead (Oncorhynchus mykiss), 36 adult bull trout (Salvelinus confluentus); 108 adult and 3 jack spring chinook (O. tshawytscha) enumerated at the Nursery Bridge Dam fishway video counting window between December 21, 2003, and June 30, 2004. Summer steelhead and spring chinook were observed moving upstream while bull trout were observed moving both upstream and downstream of the facility. In addition, the old ladder trap was operated by the WWBNPME project in order to radio tag spring chinook adults. A total of 2 adult summer steelhead, 4 bull trout, and 23 adult spring chinook were enumerated at the west ladder at Nursery Bridge Dam during the trapping operations between May 6 and May 23, 2004. Operation of the Little Walla Walla River juvenile trap for trap and haul purposes was not necessary this year. The project transported adult spring chinook from Threemile Dam to the South Fork Walla Walla Brood Holding Facility. A total of 239 spring chinook were outplanted in August for natural spawning in the basin.
A hydroelectric project located on Ashlu Creek, halfway between Whistler and Vancouver in British Columbia, consisted of a run-of-river project that used a short stretch of steep rapids to generate a capacity of 49.9MW. This paper presented the design and the optimization of the hydraulic structures during the construction phases using 2-D and 3-D numerical modelling. The proposed works included an emergency spillway weir equipped with an Obermeyer gate, a rock-fill weir, a Denil type fish ladder, a sluiceway and a side intake. The design and the verification of the upstream works were carried out using these models. The hydraulic conditions during construction phases were also simulated using the models in order to estimate the impact of the operations and validate the diversion works. It was found that numerical modeling can be an efficient alternative to small-scale physical modeling for specific applications in designing hydraulic structures.
Evaluation of Fish Passage Sites in the Walla Walla River Basin, 2008
In 2008, Pacific Northwest National Laboratory evaluated the Hofer Dam fish screen and provided technical assistance at two other fish passage sites as requested by the Bonneville Power Administration, the Walla Walla Watershed Council, or the Confederated Tribes of the Umatilla Indian Reservation. Evaluation of new sites such as Hofer Dam focuses on their design, construction, operation, and maintenance to determine if they effectively provide juvenile salmonids with safe passage through irrigation diversions. There were two requests for technical assistance in 2008. In the first, the Confederated Tribes of the Umatilla Indian Reservation requested an evaluation of the Nursery Bridge fish screens associated with the fish ladder on the east side of the Walla Walla River. One set of brushes that clean the screens was broken for an extended period. Underwater videography and water velocity measurements were used to determine there were no potential adverse effects on juvenile salmonids when the west set of screens was clean enough to pass water normally. A second request, received from the National Marine Fisheries Service and the Walla Walla Watershed Council, asked for evaluation of water velocities through relatively new head gates above and adjacent to the Eastside Ditch fish screens on the Walla Walla River. Water moving through the head gates and not taken for irrigation is diverted to provide water for the Nursery Bridge fish ladder on the east side of the river. Elevations used in the design of the head gates were incorrect, causing excessive flow through the head gates that closely approached or exceeded the maximum swimming burst speed of juvenile salmonids. Hofer Dam was evaluated in June 2008. PNNL researchers found that conditions at Hofer Dam will not cause impingement or entrainment of juvenile salmonids but may provide habitat for predators and lack strong sweeping flows to encourage juvenile salmonid passage downstream. Further evaluation of velocities at the Eastside Ditch and wasteway gates should occur as changes are made to compensate for the design problems. These evaluations will help determine whether further changes are required. Hofer Dam also should be evaluated again under more normal operating conditions when the river levels are typical of those when fish are emigrating and the metal plate is not affecting flows.
Yakima River Basin Fish Passage Phase II Fish Screen Construction, Project Completion Report.
On December 5, 1980, Congress passed the Pacific Northwest Electric Power Planning and Conservation Act (Public Law 96-501). The Act created the Northwest Power Planning Council (now the Northwest Power and Conservation Council). The Council was charged with the responsibility to prepare a Regional Conservation and Electric Power Plan and to develop a program to protect, mitigate, and enhance fish and wildlife including related spawning grounds and habitat on the Columbia River and its tributaries. The Council adopted its Fish and Wildlife Program on November 15, 1982. Section 800 of the Program addresses measures in the Yakima River Basin. The Yakima measures were intended to help mitigate hydroelectric impacts in the basin and provide off-site mitigation to compensate for fish losses caused by hydroelectric project development and operations throughout the Columbia River Basin. The Bonneville Power Administration (BPA) was designated as a major source of funding for such off-site mitigation measures and was requested to initiate discussions with the appropriate Federal project operators and the Council to determine the most expeditious means for funding and implementing the program. The primary measures proposed for rapid implementation in the Yakima River basin were the installation of fish passage and protective facilities. Sec. 109 of The Hoover Power Plant Act of 1984, authorized the Secretary of the Interior to design, construct, operate, and maintain fish passage facilities within the Yakima River Basin. Under Phase I of the program, improvements to existing fish passage facilities and installation of new fish ladders and fish screens at 16 of the largest existing diversion dams and canals were begun in 1984 and were completed in 1990. The Yakima Phase II fish passage program is an extension of the Phase I program. In 1988, the Yakama Nation (YN) submitted an application to amend Sections 803(b) and 1403(4.5) of the Northwest Power and Conservation Council's Columbia River Basin Fish and Wildlife Program to begin preliminary design on the Phase II fish screen program. Based on citizen and agency endorsement, the Council approved the amendment in 1989. The Council authorized BPA to provide funding for Phase II screens through the Fish and Wildlife Program. BPA then asked the Bureau of Reclamation to provide engineering and design expertise to the Phase II projects.
Template-assisted Formation of Charge-transfer Complex by a Mixed Supramolecular Ladder Complex
A 2,6-naphthalenediol-modified BisPYBOX (2,6-bis(benzoxazol-2-yl)pyridine (benzoPYBOX)) ligand and a naphthaldiimide-modified BisPYBOX ligand were newly designed and synthesized as a bar for ladder structure formation. The mixing of these two BisPYBOX ligands formed supramolecular ladders along a polymeric secondary dialkylammonium cations as templates to yield charge-transfer (CT) complexes in solution.
Occurrence of cyprinids in fish ladders in relation to flow
To analyse the relationship between spawning migrations and flow, cyprinids from ten fish ladders of the Elbe River (Czech Republic) were collected during the period of spawning migrations (April?June) in 1996, 2000 and 2001. Number of species and individuals in the fish ladders increased with increasing flow up to medium values, followed by further decrease during high flows, whilst length of individuals increased with increasing flow. Moreover, occurrence of four the most numerous species (bleak, roach, silver bream and barbel) showed species specific differences in response to flow variability. In early spring, the highest number of bleak in fish ladders occurred during the low flow, whilst later increased with increasing flow. Occurrence of roach and silver bream achieved the maximum d...
NB Power`s fish handling facilities
Fish handling facilities at the various hydroelectric power generating stations of New Brunswick Power on the St. John River, were described. The facilities described included the fish hoist at the Beechwood Generating Station , the fish ladder at the Tobique Generating Station and a trap and truck operation at the Mactaquac Station. Details of operating arrangements were provided. Operating issues, solutions and ongoing efforts to improve operations further, were discussed. 4 refs., 4 figs.
Walla Walla River Basin Fish Screens Evaluations, 2006 Annual Report.
Pacific Northwest National Laboratory evaluated Gardena Farms, Little Walla Walla, and Garden City/Lowden II Phase II fish screen facilities and provided underwater videography beneath a leaking rubber dam in the Walla Walla River basin in 2006. Evaluations of the fish screen facilities took place in early May 2006, when juvenile salmonids are generally outmigrating. At the Gardena Farms site, extended high river levels caused accumulations of debris and sediment in the forebay. This debris covered parts of the bottom drum seals, which could lead to early deterioration of the seals and drum screen. Approach velocities were excessive at the upstream corners of most of the drums, leading to 14% of the total approach velocities exceeding 0.4 feet per second (ft/s). Consequently, the approach velocities did not meet National Marine Fisheries Service (NMFS) design criteria guidelines for juvenile fish screens. The Little Walla Walla site was found to be in good condition, with all approach, sweep, and bypass velocities within NMFS criteria. Sediment buildup was minor and did not affect the effectiveness of the screens. At Garden City/Lowden II, 94% of approach velocities met NMFS criteria of 0.4 ft/s at any time. Sweep velocities increased toward the fish ladder. The air-burst mechanism appears to keep large debris off the screens, although it does not prevent algae and periphyton from growing on the screen face, especially near the bottom of the screens. In August 2006, the Gardena Farm Irrigation District personnel requested that we look for a leak beneath the inflatable rubber dam at the Garden City/Lowden II site that was preventing water movement through the fish ladder. Using our underwater video equipment, we were able to find a gap in the sheet piling beneath the dam. Erosion of the riverbed was occurring around this gap, allowing water and cobbles to move beneath the dam. The construction engineers and irrigation district staff were able to use the video footage to resolve the problem within a couple weeks. We had hoped to also evaluate the effectiveness of modifications to louvers behind the Nursery Bridge screens when flows were higher than 350 cubic feet per second, (cfs) but were unable to do so. Based on the one measurement made in early 2006 after the modified louvers were set, it appears the modified louvers may help reduce approach velocities. The auxiliary supply water system gates also control water through the screens. Evaluating the effect of different combinations of gate and louver positions on approach velocities through the screens may help identify optimum settings for both at different river discharges.
The LHCb silicon inner tracker
The inner part of the LHCb tracking system will be realised in a silicon microstrip technology. The experimental requirements suggest 20 cm long ladders and a readout pitch of around 240 mum. The complete LHCb Inner Tracker system will consist of nine stations with an overall silicon surface area of approximately 14 m**2, depending on the final layout of the tracking system. A report about the current design of the silicon ladders and tracking stations of the Inner Tracker is given. First characterisations of prototype sensors and test beam results of ladders are presented.
Walla Walla River Fish Passage Operations Program, 2002-2003 Annual Report.
In the late 1990's, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate fish migration conditions in the basin. The migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow requirements, and initiating trap and haul efforts. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survival of migrating adult and juvenile salmonids in the Walla Walla River basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage criteria and passage and trapping facility design and operation. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. During the 2002-2003 project year, there were 545 adult summer steelhead (Oncorhynchus mykiss), 29 adult bull trout (Salvelinus confluentus); 1 adult and 1 jack spring chinook (O. tshawytscha) enumerated at the Nursery Bridge Dam fishway adult trap between January 1 and June 23, 2003. Summer steelhead and spring chinook were observed moving upstream while bull trout were observed moving both upstream and downstream of the facility. Operation of the Little Walla Walla River juvenile trap for trap and haul purposes was not necessary this year. The project transported 21 adult spring chinook from Ringold Springs Hatchery and 281 from Threemile Dam to the South Fork Walla Walla Brood Holding Facility. Of these, 290 were outplanted in August for natural spawning in the basin.
Operational Test Report (OTR) for U-103 Pumping and Instrumentation and Control (PIC) Skid
Attached is the completed Operation Test Procedure (OTP-200-004, Rev. A-16). OTP includes a print out of the Programmable Logic Controller (PLC) Ladder Diagram. Ladder Diagram was designed for installation in the PLC used to monitor and control pumping activity for Tank Farm 241-U-103. The completed OTP and OTR are referenced in the 25 PIC Skid Configuration Drawing (H-2-829998).
Current mode leapfrog ladder filters using a new active block
In this paper, a new multi-terminal active component, namely current backward transconductance amplifier (CBTA) is proposed. The design procedure for the systematic realization of current-mode ladder filters using proposed active component is also presented. In order to demonstrate that the CBTA considerably simplifies the leapfrog structure of the current-mode ladder filters, a fifth-order Butterworth low-pass filter that requires a minimum of active components is presented. The theoretical results are demonstrated by using PSPICE simulation.
Nurse leaders' views on clinical ladders as a strategy in professional development
torstad s. &bjork i. t. (2007) Journal of Nursing Management15, 817-824 Nurse leaders' views on clinical ladders as a strategy in professional development Aim To investigate nurse leaders' views on clinical ladders as a strategy in professional development of nursing. Background Hospitals worldwide have implemented clinical ladders to boost professional development and improve quality of patient care. At ward level good leadership is vital in creating a learning environment and using nurses' new competence. Method The design was explorative and data were collected in focus groups with 19 nurse leaders at the ward level and 24 executive nurse leaders at hospitals with several years of experience with clinical ladders. Results Most nurse leaders did not think strategically in promoting clini...
Tests of prototype ITS-SSD Ladder and Fixators
Abstract A prototype carbon fibre SSD ladder, produced in St.Petersburg, was equipped in Utrecht with mounting blocks, designed in Torino, for the fixation on the ITS cone. Instead of the cone, a specially designed jig was used. The mechanical properties were measured in order to find out whether the design and the production method are compliant with the requirements given in the TDR.
Due to hydropower development, the upstream migration of wild anadromous salmon and brown trout is impaired in many European rivers, causing negative effects on the long-term survival of natural salmonid populations. This study identified problems for Atlantic salmon during upstream migration in a regulated river in northern Sweden, Ume?lven (mean flow: 430?m3?s?1). Tagging from 1995 to 2005 involved radio tags (n?=?503), PIT tags (n?=?1574) and Carlin tags (n?=?573) to study the spawning migration of salmon from the coast past the regulated section of the river to a fish ladder at the dam/spillway 32?km upriver. The results demonstrate that migration success from the coast to the fish ladder varied between 0% and 47% among years, indicating an average loss of 70% of potential spawners. Di...
Selectivity of fish ladders: a bottleneck in Neotropical fish movement
Abstract in portuguese Dezenas de escadas de peixes foram construídas em barragens de reservatórios brasileiros, mas são raros os estudos acerca de suas eficiências como instrumentos de conservação da ictiofauna Neotropical, em especial de espécies migradoras. Neste contexto, o presente estudo teve como objetivo avaliar a seletividade específica no ingresso e ascensão de peixes na escada localizada junto à barragem de Lajeado (UHE Luis Eduardo Magalhães, rio Tocantins). Amostragens f (more) oram realizadas mensalmente de novembro de 2002 a outubro de 2003 nos tanques de descanso da escada, utilizando tarrafas, e no trecho a jusante, utilizando redes de espera. A avaliação da seletividade no ingresso da escada foi realizada através da comparação da ocorrência, abundância relativa, dominância e congruência dos ranks de abundância de espécies migradoras e não migradoras na escada e no trecho imediatamente a jusante. A riqueza e abundância específica nos diferentes tanques de descanso foram utilizadas para avaliar a seletividade ao longo da escada. Os efeitos das variações temporais do nível hidrométrico de jusante e da velocidade de fluxo na seletividade foram também analisados. Das 130 espécies registradas a jusante, 63,2% foram capturadas na escada, com claro favorecimento das espécies migradoras. Entretanto, mais de 2/3 das capturas pertenceram a apenas três espécies (Rhaphiodon vulpinus, Psectrogaster amazonica e Oxydoras niger). Embora a maioria das espécies que ingressa na escada possa alcançar seu topo, constatou-se uma redução pronunciada na abundância. Variações temporais no nível da água a jusante da barragem influenciaram a riqueza e a abundância de peixes que se concentram na entrada e dentro da escada, com redução nos valores durante períodos de níveis baixos. Já na escada, a velocidade de fluxo de 2,3 m.s-1, embora também seletiva, mostrou-se mais adequada à ascensão de peixes em relação a de 2,8 m.s-1. Conclui-se, portanto, que o ingresso e a ascensão de peixes na escada não são congruentes com a composição e relações de abundância das espécies no trecho a jusante. O conjunto de espécies que efetivamente ascende a escada é dominado por poucas espécies, podendo incluir entre as dominantes aquelas que não necessitam ser transpostas. Dessa maneira, a seletividade se constitui num importante gargalo nas iniciativas de transposição de peixes visando a conservação dos estoques ou da biodiversidade, sendo urgente a revisão do processo decisório sobre a construção destes dispositivos e a avaliação acerca do funcionamento daquelas já construídas. Abstract in english Although dozens of fish ladders have been constructed at dams of Brazilian reservoirs, there are few studies evaluating their efficiency as a tool for the conservation of Neotropical ichthyofauna, especially for migratory species. Therefore, the present study evaluated the selectivity of the species that entered and ascended the fish ladder located next to Lajeado Dam (Luis Eduardo Magalhães Hydroelectric Power Plant) on the Tocantins River. Samples were taken monthly fr (more) om November, 2002 through October, 2003, in the resting pools of the ladder, using cast nets, and in the downstream stretch, using gillnets. The selectivity of the ladder in attracting fish was evaluated by comparing the occurrence, relative abundance, dominance and the congruence of abundance ranks of migratory and non-migratory species in the ladder and in the stretch of river immediately downstream. Species richness and fish abundance in the resting pools were used to evaluate selectivity along the ladder. The effects on selectivity by temporal variations in water level downriver and maximum flow velocity in the fish ladder were also analyzed. Out of the 130 species recorded downriver, 62.3% were caught in the ladder, and migratory species were clearly favored. However, more than 2/3 of the catch belonged to only three species (Rhaphiodon vulpinus, Psectrogaster amazonica and Oxydoras niger). Although the majority of the species that entered the ladder were able to reach its top, there was a sharp reduction in abundance of individuals towards the top. Temporal variations in the water level below the dam influenced richness and abundance of fish concentrated downstream and in the ladder, with lower values during periods of low water. In the ladder, a maximum flow velocity of 2.3 m/s, although also selective, proved to be more appropriate for fish ascension than a velocity of 2.8 m/s. It was concluded that the entry and ascension of the fish in the ladder were not congruent with their proportions in the downriver stretch: fish samples in the ladder were clearly dominated by a few species, including some that do not need to be translocated. Thus, selectivity constitutes an important bottleneck to initiatives for translocating fish aimed at conserving their stocks or biodiversity. It is urgent to review the decision-making process for the construction of fish passages and to evaluate the functioning of those already operating.
Abstract in portuguese Nesse estudo, a transposição de peixes na escada experimental do reservatório de Itaipu (Paraná, Brasil) foi avaliada em relação à variação diária, tamanho dos peixes, estágios reprodutivos e a composição das espécies. O estudo foi conduzido de Outubro de 1995 a Fevereiro de 1996, e de Novembro de 1996 a Janeiro de 1997, em dois trechos da escada (caixas a 10 e 27 m de altura). Vinte e duas espécies foram registradas, e Prochilodus lineatus, Pimelodus macul (more) atus, Leporinus obtusidens, Schizodon borellii e Leporinus friderici compuseram 86,6% das capturas. O tamanho dos peixes não diferiu entre os períodos do dia e caixas. Por outro lado, a abundância das principais espécies foi significativamente influenciada pela posição das caixas, estágio de reprodução e período do dia. A caixa superior tendeu a apresentar menor abundância de peixes, sugerindo certa seletividade na subida. A habilidade em ascender a escada não esteve relacionada com a migração reprodutiva, visto que adultos não-reprodutivos compuseram a maior parte das capturas. Um padrão interessante foi a preferência, apresentada pelos grandes grupos taxonômicos, por períodos específicos do dia na ascensão da escada: os Characiformes subiram a escada predominantemente durante o dia, enquanto os Siluriformes durante a noite. Esses resultados enfatizam o quão complexas são as escadas como ferramentas de manejo, e realçam a necessidade de ampliar o nível de informações sobre diversos aspectos como condição chave para que metas conservacionistas sejam alcançadas. Abstract in english In this study, we evaluated the fish ascent in the experimental ladder at Itaipu Reservoir (Paraná State, Brazil), in relation to diel variation, fish size, reproductive stages and species composition. The study was carried out from October, 1995 to February, 1996 and from November, 1996 to January, 1997 at two points on the ladder (boxes at heights of 10 and 27 m). Twenty-two species were recorded, and Prochilodus lineatus, Pimelodus maculatus, Leporinus obtusidens, Sch (more) izodon borellii and Leporinus friderici accounted for 86.6 % of the total catch. Fish length did not differ between diel periods and boxes. On the other hand, fish abundance of the main species was significantly influenced by box position, reproductive stage and diel period. The higher box showed lower abundance, suggesting some selectivity in ascension. The ability to ascend the ladder did not show any relationship with reproductive migration, since non-reproductive adults comprised most of the catch. An interesting finding is that the major taxonomic groups showed specific diel preferences in ascending the ladder: Characiformes ascended the ladder predominantly during daylight, whereas Siluriformes migrated at night. These results emphasize how complex ladders are as management tools, and stress the need to acquire information on several aspects as a condition to achieve conservation goals.
Movements through a fish ladder: temporal patterns and motivations to move upstream
Abstract in portuguese Os mecanismos de transposição de peixes em barragens visam restabelecer a conectividade entre habitats críticos à manutenção para as espécies que tenham uma ampla área de vida. Os movimentos ascendentes da maioria das espécies migradoras conhecidas são realizados por motivação reprodutiva (adultos) ou dispersiva (juvenis). Neste contexto, o presente estudo tem como objetivo avaliar se existe um padrão temporal na abundância de peixes a jusante da barragem e (more) na escada da UHE Lajeado (UHE Luis Eduardo Magalhães, rio Tocantins) que segue a sazonalidade reprodutiva. Adicionalmente, busca avaliar se reprodução é a motivação predominante dos cardumes que ascendem a escada. Para isto, as amostragens foram realizadas mensalmente de novembro de 2002 a outubro de 2003 a jusante da barragem, com o uso de redes de espera, e nos tanques de descanso da escada, com tarrafas. A análise da sazonalidade na agregação dos cardumes foi baseada nas variações temporais de abundância e riqueza de espécies, enquanto a motivação reprodutiva para a ascensão foi inferida a partir da freqüência dos estádios de maturação gonadal. Entretanto, durante todo o período foi constatada uma elevada freqüência de indivíduos com gônadas em fase pré-vitelogênica em todas as espécies analisadas, tanto a jusante da barragem (97%) como na escada (98%), sugerindo a inexistência de motivação reprodutiva para a agregação dos peixes a jusante e seus ingressos na escada. Por outro lado, o fato destes estádios dominarem as capturas também a jusante sugere que os locais de desova estão distantes, e que os deslocamentos ascendentes ocorrem por outras razões, como dispersão natural e busca de habitats mais apropriados para alimentação e crescimento. Assim, o ingresso na escada decorreu mais do comportamento reofílico, exacerbado nas espécies migradoras, que de uma motivação endógena ligada à migração reprodutiva. Abstract in english Fish passages are constructed with the aim of reestablishing connectivity between critical habitats, in order to sustain those species that move over a large area during their lives. The upstream movements of most of the known migratory species are made for purposes of reproduction (adults) or dispersal (juveniles). The present study was conducted at the ladder constructed at Lajeado Dam (Luis Eduardo Magalhães Hydroelectric Power), on the Tocantins River. The objective (more) was to assess whether there is a temporal pattern in the abundance of fish below the dam and in the ladder, that correlates with their breeding seasons. Additionally, it was examined whether reproduction is the predominant motivation of the shoals of fish that ascend the ladder. Samples were taken monthly from November, 2002 through October, 2003, downriver from the dam with gillnets, and in the resting pools of the fish ladder with cast nets. The analysis of seasonality in the aggregation of the shoals was based on the temporal variations in abundance and species richness. The possibility of a reproductive motivation for ascending the ladder was inferred from the frequency of the stages of gonadal development. However, during the entire study period we observed a high frequency of individuals with gonads in the pre-vitellogenic stage for all analyzed species, both downriver from the dam (97%) and in the fish ladder (98%). These findings suggest that there was no reproductive motivation for the aggregation of the fish downstream and for their entry into the ladder. On the other hand, the dominance of these stages downriver suggests that the spawning habitats are distant, and that the upstream movements occur for other reasons such as dispersal and search for more appropriate habitats for feeding and growth. The entrance into the ladder resulted more from rheophilic behavior, which is more pronounced in migratory species, than from an endogenous motivation linked to reproductive migration.
Umatilla Basin Fish Facilities Operation & Maintenance : Annual Report Fiscal Year 2008.
Westland Irrigation District, as contractor to Bonneville Power Administration, and West Extension Irrigation District, as subcontractor to Westland, provide labor, equipment, and material necessary for the operation, care, and maintenance of fish facilities on the Umatilla River. Westland Irrigation District is the contractor of record. Job sites that are covered: Three Mile Right, Three Mile Left, Three Mile Adult Spawning, WEID Sampling Facility, Maxwell Screen Site, Westland Screen Site/Ladder/Juvenile Sampling Facility, Feed Canal Ladder/Screen Site, Stanfield Ladder/Screen Site, Minthorn Holding Facility, Thornhollow Acclimation Site, Imeques Acclimation Site, Pendleton Acclimation Site, and South Fork Walla Walla Spawning Facility. O & M personnel coordinate with the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) and Oregon Department of Fish and Wildlife (ODFW) personnel in performing tasks under this contract including scheduling of trap and haul, sampling, acclimation site maintenance, and other related activities as needed. The input from ODFW biologists Bill Duke and Ken Loffink, and CTUIR biologist Preston Bronson is indispensable to the success of the project, and is gratefully acknowledged. All tasks associated with the project were successfully completed during the fiscal year 2008 work period of October, 2007 through September, 2008. The project provides operations and maintenance throughout the year on five fish screen sites with a total of thirty-four rotating drum-screens, and four fish ladders in the Umatilla River Basin; additionally, periodic operations and maintenance is performed at holding, acclimation, and spawning sites in the Basin. Three people are employed full-time to perform these tasks. The FY08 budget for this project was $492,405 and actual expenditures were $490,267.01. Selected work activities and concerns: (1) Feed Dam Passage Improvement Project - A project to improve fish passage over the short term at the Feed Canal Diversion Dam site (Umatilla River mile 28.7) was implemented with local U.S. Bureau of Reclamation field office personnel coordinating project activities. Operation and Maintenance Project personnel assisted with labor, materials, and equipment to ensure the project was completed as planned. Discussions are under way to determine feasible alternatives for longer term solutions to passage issues at the site. (2) Three Mile Right (east bank) Facilities - The pump for supplying water to the fish handling facility holding pond was reconstructed successfully to achieve the desired increase in flow output necessary when increased quantities of fish are present. (3) Fish Screen Rehab at Stanfield Canal Diversion - Working with the Washington Department of Fish and Wildlife screen shop personnel, three rotating drum screens at the Stanfield Canal diversion site (Umatilla River mile 33.3) were serviced and overhauled with new bearings, seals, paint, and reinforcing bars. Work was completed, and screens reinstalled prior to water diversions beginning in the spring. (4) O & M personnel performed daily, weekly, and monthly operations and maintenance duties at the screen and ladder sites including, but not limited to, desilting of mud and debris, lubrication of mechanical parts, replacement of screen seals and screen motor components, adjustment of ladder gates, removal of large trees and woody debris deposited during high river flow conditions, servicing of pumps and screens for fish handling operations and sampling studies, in addition to general site clean-up, vegetative control, and security. Crew members responded as needed during evenings and after-hours according to weather conditions, river flows, and fish passage facility needs.
The ladder sleeper developed by Railway Technical Research Institute is a next-generation rail supporting component of the longitudinal sleeper type. The concrete-made longitudinal left-side and right-side beams, parallelly arranged, are connected with each other by steel-made joining pipes, and consequently assume a ladder-like external appearance. Since the concrete-made longitudinal beams are so designed as to support and restrain the rails continuously, it may be said that the result is a `composite rail` with a superior load carrying capacity and high lateral track stability. The ballasted ladder tracks are expected to suffer less from deterioration to be caused by worn ballast. The floating ladder tracks will be a technological breakthrough in the field of non-ballasted tracks, to eventually replace the conventional slab tracks. The floating ladder tracks will enable the construction of innovative type viaducts excellent in cost performance, friendliness towards environments, and resistance to earthquake. It is expected that the new system upon adoption will bring about a great progress in the railway business. 35 refs., 24 figs.
There are two parts to this page. The first part is for the Electromagnetic Spectrum Project. The second is for the Town Meeting. Find the links for your specific group below in this second section. CSI : The Experience Science Spot Calorimetry Lab PhET:Physics Educational Technology PART ONE - Electromagnetic Spectrum Project NASA - Imagine the Universe! Micro Worlds - Electromagnetic Spectrum The Electromagnetic Spectrum Amazing Space - ElectroMagnetic Spectrum Discovery Education - Tutorial PART TWO TOWN MEETING CHAMBER OF COMMERCE Bait Tackle SCIENTISTS Heavy Metal Effects ENGINEERS How a dam works Hydroelectric power: How it works Spillways Fish Ladders Mining Waste Storage Basin POWER COMPANY How a dam works Hydroelectric power: How it works Spillways Fish Ladders MINING COMPANY What is Mining? Silver Mining New Jersey Zinc Company COUNTY SANITATION COMMISSION Drinking Water Contaminants Water Quality Testing FARMERS EPA Pesticide - National Pesticide Information Florida Fish and Wildlife - Fish Kill Causes DAVAO RIVER FISHKILL CAUSED BY POISONS Buzzard Bay - Pesticide Information CBCnews.ca -P.E.I. says fish kills in rivers could be extensive ...
Research into the environmental effects of hydroelectric power production in the United States has focused increasingly on resident and migratory fish populations. Hydropower dams and reservoirs can block fish movements in both upstream and downstream directions. These movements are essential for important stocks of anadromous and catadromous fish. In addition, some strictly freshwater fish may move long distances within a river during their life cycle.A dam can pose an impassable barrier for fish trying to move upstream unless mitigation measures in the form of ladders or lifts are provided. Fish moving downstream to the sea may become disoriented when they encounter static water within a reservoir. Both resident and migratory fish may be injured or killed by passing through the turbine or over the spillway. In the United States, a variety of organizations conduct applied research and development of measures to (1) enhance fish passage, (2) reduce the numbers of fish that are drawn into the turbine intakes, and (3) reduce the injury and mortality rates of fish that pass through the turbines. Examples of these efforts from a variety of river systems and hydroelectric power plants are described.
Phonon Bloch oscillations in acoustic-cavity structures
We describe a semiconductor multilayer structure based in acoustic phonon cavities and achievable with MBE technology, designed to display acoustic phonon Bloch oscillations. We show that forward and backscattering Raman spectra give a direct measure of the created phononic Wannier-Stark ladder. We also discuss the use of femtosecond laser impulsions for the generation and direct probe of the induced phonon Bloch oscillations. We propose a gedanken experiment based in an integrated phonon source-structure-detector device, and we present calculations of pump and probe time dependent optical reflectivity that evidence temporal beatings in agreement with the Wannier-Stark ladder energy splitting.
Abstract in portuguese Neste artigo, contamos os curimbatás na escada de peixes da Cachoeira de Emas, de Outubro/92 a Setembro/93, num dia durante 24h por mês, em diferentes pontos de observação da barragem, durante 5 min a cada hora. A maioria dos peixes (80%) foi observada e contada não na escada de peixes, mas em dois vertedouros adjacentes à sua esquerda, talvez devido ao maior fluxo de água, pois são mais estreitos que a escada. O pico da migração ocorreu em Setembro/Outubro. Ins (more) pecionamos o grau de injúria dos peixes a fim de examinar a hipótese de que peixes maiores estariam menos feridos, porém não chegamos a uma conclusão clara. O grau de injúria variou entre diferentes pontos e entre diferentes meses do ano. Abstract in english In this paper, we counted the ascending curimbatás at the fish ladder at the Cachoeira de Emas from October, 1992 to September, 1993 in one whole day (24 h period) per month at different observation points in the dam for 5 min every hour. Most of the fish (80%) were counted not in the fish ladder itself, but in two adjacent spillways at its left, perhaps due to the comparatively increased flow as they are narrower than the ladder. The bulk of the migration occurred in Se (more) ptember/October. We inspected the degree of injury of the fish in order to examine the hypothesis that larger fish are less injured, however no conclusion was reached. The degree of injury varied between different points and in different months of the year.
Executive summary 1) The means-end chain model has been widely advocated for the understanding of how consumers perceive self-relevant consequences of products. The model implies that subjective product meaning is established by associations between product attributes and more abstract, more central cognitive categories like values. A means-end chain consists of concrete product attributes, abstract product attributes, functional consequences, psychosocial consequences, instrumental values, a terminal values. 2) The most common method of measuring means-end chains has been the laddering interview technique. Laddering is a semi-qualitative technique which is open for the respondent's own answers without flooding the interviewer with data, as do other qualitative techniques. The results from the laddering interviews are coded and usually presented in a so-called hierarchical value map. 3) However popular, a number of problems with means-end chain analysis have been pointed out. In this paper we report how threevariables affect the outcome of a laddering study, namely (1) product experience, (2) product involvement, and (3) verbal processing style. 4) 90 respondents in the Copenhagen area were interviewed. Respondents were asked to rank four meat types, ie, (1) a fresh, whole, gutted plaice, (2) a package of frozen fish fillets, (3) a whole, frozen chicken, and (4) a package of fresh pork chops, according to how likely it would be that they would use them for a hot meal on a work Respondents were asked to give reasons for the ranking, and from the answers given, laddering procedures were carried out. 5) At the aggregate level, ie when comparing hierarchical value maps across different groups of consumers, the investigation showed that less involved consumers perceived more negative consequences of the consumption of fresh plaice than did the more involved consumers. Fresh fish is perceived to be difficult to get hold of it has to be bought at the fishmonger's di to prepare, and difficult to eat. These aspects are considered time-consuming and result in less time spent with the family. Both the less involved and the more involved consumers regard fresh plaice as healthy. 6) At the individual level, ie when comparing the amount and character of the data provided by the individual respondents, we found that consumers' level of experience had a significant positive relationship with the number of ladders retrieved in the interviews and a significant ne relationship with the proportion of consequence categories. There was no effect on the value categories' share of the total number of categories. As for consumers' level of involvement we found a significant positive correlation with the number of ladders provided in the interviews. Consumers' tendency towards a verbal processing style seemed to have no influence on neither the number of ladders, average length of the ladders, nor the proportion of value categories in the data set. 7) The study seems to indicate that it is possible to employ laddering with consumers withboth higher and lower degrees of involvement and experience. The study also showed that the respondent's capability of verbal processing had no influence on the results. method can therefore not be said to be more or less applicable to respondents with varying degrees of verbal processing ability.
Diagnostic PCR to identify five rare species of Cypriniformes in China
Abstract The major problem that fisheries managers face when trying to enforce the law is identifying fish species. This problem is even worse when it comes to identifying eggs, larvae, bloodstains, scales, mucous stains, and mixtures of these in markets, restaurants and fishing areas. To assist in the acquisition of urgently needed conservation and management data on rare and endangered fish catch and sale, we have developed and tested a highly streamlined molecular genetic approach to identify Cypriniformes fish species. We used species-specific primers and species-specific ladders in a seven-primer multiplex polymerase chain reaction format based on DNA sequence differences among species in the nuclear ribosomal second internal transcribed spacer (ITS2) to discriminate simultaneously am...
With the completeness of genome databases, it has become possible to develop a novel FISH (Fluorescence in Situ Hybridization) technique called COMBO-FISH (COMBinatorial Oligo FISH). In contrast to other FISH techniques, COMBO-FISH makes use of a bioinformatics approach for probe set design. By mean...
Hood River and Pelton Ladder Evaluation Studies, Annual Report 2000-2001.
The Bonneville Power Administration (BPA) funded the development of two master plans which outline the rationale, and general approach, for implementing a defined group of projects that are an integral part of a comprehensive watershed goal to 'Protect, enhance and restore wild and natural populations of anadromous and resident fish within the Hood River Subbasin'. The Hood River Production Master Plan and the Pelton Ladder Master Plan were completed in 1991 and subsequently approved by the Northwest Power Planning Council in 1992. Action items identified in the two master plans, as well as in a later document entitled 'Hood River/Pelton Ladder Master Agreement' (ODFW and CTWSRO Undated), are designed to achieve two biological fish objectives: (1) to increase production of wild summer and winter steelhead (Oncorhynchus mykiss) to levels commensurate with the subbasins current carrying capacity and (2) re-establishing a self-sustaining population of spring chinook salmon (Oncorhynchus tshawytscha). Numerical fish objectives for subbasin escapement, spawner escapement, and subbasin harvest are defined for each of these species in Coccoli (2000). Several projects are presently funded by the BPA to achieve the Hood River subbasin's numerical fish objectives for summer and winter steelhead and spring chinook salmon. They include BPA project numbers 1998-021-00 (Hood River Fish Habitat), 1998-053-03 (Hood River Production Program - CTWSRO: M&E), 1998-053-07 (Parkdale Fish Facility), 1998-053-08 (Powerdale/Oak Springs O&M), and 1998-053-12 (Hood River Steelhead Genetics Study). Collectively, they are implemented under the umbrella of what has come to be defined as the Hood River Production Program (HRPP). The HRPP is jointly implemented by the Oregon Department of Fish and Wildlife (ODFW) and The Confederated Tribes of the Warm Springs Reservation of Oregon (CTWSRO). Strategies for achieving the HRPP's biological fish objectives for the Hood River subbasin were initially devised based on various assumptions about (1) subbasin carrying capacity, (2) survival rates for selected life history stages, and (3) historic and current escapements of wild, natural, and hatchery stocks of anadromous salmonids to the Hood River subbasin. The Oregon Department of Fish and Wildlife began funding a monitoring and evaluation (M&E) project in December 1991 to collect the quantitative biological information needed to (1) more accurately assess the validity of these assumptions and (2) evaluate the proposed hatchery supplementation component of the HRPP. Bonneville Power Administration assumed funding of the M&E project in August 1992. The M&E project was initially confined to sampling anadromous salmonids escaping to an adult trapping facility operated at Powerdale Dam; which is located at River Mile (RM) 4.5 on the mainstem of the Hood River. Stock specific life history and biological data was collected to (1) monitor subbasin spawner escapements and (2) collect pre-implementation data critical to evaluating the newly proposed HRPP's potential biological impact on indigenous populations of resident fish. The scope of the M&E project was expanded in 1994 to collect the data needed to quantify (1) subbasin smolt production and carrying capacity, (2) smolt to adult survival rates, and (3) the spatial distribution of indigenous populations of summer and winter steelhead, spring and fall chinook salmon, and coho salmon. A creel was incorporated into the M&E project in December 1996 to evaluate the HRPP with respect to its defined subbasin and spawner escapement objectives for Hood River stocks of wild and hatchery summer and winter steelhead and for natural and Deschutes stock hatchery spring chinook salmon. In 1996, the M&E project also began monitoring streamflow at various locations in the Hood River subbasin. Streamflow data will be used to correlate subbasin smolt production with summer streamflows. Data collected from 1991-1999 is reported in the following annual progress reports: Olsen et al. (1994), Olsen et al. (1995), Olsen and French (1996), Olsen et al. (1996), Olsen and French (1999), and Olsen and French (2000). The annual progress reports document information collected on (1) rearing densities of indigenous fish, (2) subbasin steelhead smolt production, (3) post-release survival of acclimated and direct released hatchery summer and winter steelhead smolts, (4) smolt to adult anadromous salmonid survival rates, (5) jack and adult anadromous salmonid escapements and harvest, (6) spatial distribution of adult anadromous salmonid holding in the Hood River subbasin, (7) selected life history patterns and morphological and meristic characteristics of wild, natural, and hatchery resident and anadromous salmonids, and (8) summer streamflows.
Benefits of fish passage and protection measures at hydroelectric projects
The US Department of Energy`s Hydropower Program is engaged in a multi-year study of the costs and benefits of environmental mitigation measures at nonfederal hydroelectric power plants. An initial report (Volume 1) reviewed and surveyed the status of mitigation methods for fish passage, instream flows, and water quality; this paper focuses on the fish passage/protection aspects of the study. Fish ladders were found to be the most common means of passing fish upstream; elevators/lifts were less common, but their use appears to be increasing. A variety of mitigative measures is employed to prevent fish from being drawn into turbine intakes, including spill flows, narrow-mesh intake screens, angled bar racks, and lightor sound-based guidance measures. Performance monitoring and detailed, quantifiable performance criteria were frequently lacking at non-federal hydroelectric projects. Volume 2 considers the benefits and costs of fish passage and protection measures, as illustrated by case studies for which performance monitoring has been conducted. The report estimates the effectiveness of particular measures, the consequent impacts on the fish populations that are being maintained or restored, and the resulting use and non-use values of the maintained or restored fish populations.
Synthesis and characterization of low-dimensional molecular magnetic materials
This dissertation presents experimental results from the synthesis and structural, magnetic characterization of representative low-dimensional molecule-based magnetic materials. Most of the materials reported in this dissertation, both coordination polymers and cuprate, are obtained as the result of synthesizing and characterizing spin ladder systems; except the material studied in Chapter 2, ferricenyl(III)trisferrocenyl(II)borate, which is not related to the spin ladder project. The interest in spin ladder systems is due to the discovery of high-temperature superconductivity in doped cuprates possessing ladder-like structures, and it is hoped that investigation of the magnetic behavior of ladder-like structures will help us understand the mechanism of high-temperature superconductivity. Chapter 1 reviews fundamental knowledge of molecular magnetism, general synthetic strategies for low-dimensional coordination polymers, and a brief introduction to the current status of research on spin ladder systems. Chapter 2 presents a modified synthetic procedure of a previously known monomeric complex, ferricenyl(III)trisferrocenyl(II)borate, 1. Its magnetic properties were characterized and previous results have been disproved. Chapter 3 investigates the magnetism of [CuCl2(CH3CN)] 2, 2, a cuprate whose structure consists of isolated noninterpenetrating ladders formed by the stacking of Cu(II) dimers. This material presents an unexpected ferromagnetic interaction both within the dimeric units and between the dimers, and this behavior has been rationalized based on the effect of its terminal nonbridging ligands. In Chapter 4, the synthesis and magnetism of two ladder-like coordination polymers, [Co(NO3)2(4,4'-bipyridine) 1.5(MeCN)]n, 3, and Ni2(2,6-pyridinedicarboxylic acid)2(H2O)4(pyrazine), 4, are reported. Compound 3 possesses a covalent one-dimensional ladder structure in which Co(II) ions are bridged through bipyridine molecules. Compared to the materials discussed in Chapter 3 and Chapter 4, Chapter 5 presents a strategy of synthesizing ladder-like structures in a distinctively different system, bimetallic cyanide-bridged Prussian Blue analogs. Calculations are designed and carried out to identify two potentially successful model structures which are subsequently attempted synthetically. The two model structures are [NiII(CN)4]3[FeIII(terpy)] 2 and [FeII(cyclam)2]3[Ni II(dpa)]2(CN)6. Finally, in Chapter 6, four Cu(II)-based low-dimensional materials are reported. Two of them, Cu2(C2O4)(histamine) 2[N(CN)2]2, 5, and Cu2(C 2O4)(histamine)2(N3)2, 6, consist of dimeric structural units that assemble into two-dimensional sheets. Magnetically, both behave as an isolated dimer system. On the other hand, compound 7, [Cu4(4,4'-bipyrimidine) 2(N3)8]n has a two-dimensional covalent network structure that can be viewed as being formed by covalently bridging one-dimensional ladders. (Abstract shortened by UMI.)
Lower Granite Dam on the Snake River to evaluate the feasibility of using video technology to document and estimate fish ladder passage of chinook salmon Oncorhynchus tshawytscha, sockeye salmon O. nerka, and steelhead O. mykiss. A video system was to produced video images during salmon passage periods. A technician identified and counted fish images from the video record. Fish ladder passage estimates of target species made from the video record were similar to estimates made by on-site counters during daytime periods, indicating that the two methods were relatively precise. We also found that a significant percentage (6.4% and 8.3%) of target salmonids migrated during nighttime periods when on-site counts were not typically made during the two years of study. Analysis of the video record permitted verification of individual sockeye salmon identified and counted by on-site count personnel, and provided data useful to managers of this ESA-listed stock. Analysis of the video record also permitted collection of additional data such as length measurements of individual specimens, which was used to regulate a fishery located upstream.
In Egypt, Lake Qaroun and its neighbouring fish farms are in a serious environmental situation as a result of pollution by agricultural sewage and domestic non-treated discharges. The present study aims to evaluate genotoxic effects of toxic metals in cultured and wild Nile tilapia, Oreochromis niloticus and mullet, Mugil cephalus collected from these contaminated aquatic habitats, in comparison with fish from a non-polluted reference site. Heavy-metal concentrations (Cu(2+), Zn(2+), Pb(2+), Fe(2+) and Mn(2+)) in water and sediment samples were recorded. The condition factor (CF) was taken as a general biomarker of the health of the fish, and genotoxicity assays such as the micronucleus (MN) test and a DNA-fragmentation assay were carried out on the fish species studied. In addition to micronuclei, other nuclear abnormalities (NA) were assessed in fish erythrocytes. Degradation of the studied aquatic habitats revealed species-specific effects. A significant decrease in CF values associated with a significant elevation in MN and NA frequencies was observed in fish collected from the polluted areas compared with those from the reference site. Moreover, mixed smearing and laddering of DNA fragments in gills and liver samples of both fish species collected from the polluted areas indicate an intricate pollution condition. Results of the present study show the significance of integrating a set of biomarkers to identify the effects of anthropogenic pollution. High concentrations of heavy metals have a potential genotoxic effects, and genotoxicity is possibly related to agricultural and domestic activities. PMID:22464984
Using Insects to Make Healthy Space Foods
Providing foods to space crew is the important requirements to support long term manned space exploration. Foods fill not only physiological requirements to sustain life, but psychological needs for refreshment and joy during the long and hard mission to extraterrestrial planets. We designed joyful and healthy recipe with materials (plants, insects, fish et.cet. la.), which can be produced by the bio-regenerative agricultural system operated at limited resources available in spaceship or on Moon and Mars. And we need to get the storage method of the food without the problem of food poisoning. The consideration about the food allergy is necessary, too. Nutritional analysis on the basic vegetable menu consisting of rice, barley, soybean, sweet potato cassava, quinoa and green reveals a shortage of vitamins D and B12, cholesterol and sodium salt. Since vitamin D deficiency results in demineralization of bone. Vitamin B12 is essential to prevent pernicious anemia. Fish contains both vitamins D and B12. The pupa of the silkworm becomes the important nourishment source as protein and lipid. The silk thread uses it as clothing and cosmetics and medical supplies. However, we can use the silk thread as food as protein. A law of nature shakes high quality oils and fats included in termite for cooking. I use the bee as food after having used it for the pollination of the plant. Of course the honey becomes the important food, too. The snail and mud snail become the food as protein. We decided to use the menu consisting of the basic vegetarian menu plus insect and loach for further conceptual design of space agriculture. We succeeded to develop joyful and nutritious space recipe at the end. Since energy consumption for physical exercise activities under micro-or sub-gravity is less than the terrestrial case, choice of our space foods is essential to suppress blood sugar level, and prevent the metabolic syndrome. Because of less need of agricultural resources at choosing ecological members from the lower ladder of the food chain, our space recipe could be a proposal to solve the food problem on Earth.
The Study of Importance of the Balance Space Food with Insects
Providing foods to space crew is the important requirements to support long term manned space exploration. We designed joyful and healthy recipe with materials (plants, insects, fish and et.cet.), which can be produced by the bio-regenerative agricultural system operated at limited resources available in spaceship or on Moon and Mars. The fundamental composition of our recipe is unpolished rice, barley, soybean, sweat potato and green-yellow vegetables. Supplement food materials to fulfill the nutritional requirements we chose are loach, silkworm pupa, termite, snail, mud snail, bee, locust, cassava, quinoa and et. cet. And the space foods were made by using IH cooking heater and vacuum cooking machine. Nutritional analysis on the basic vegetable menu consisting of rice, barley, soybean, sweet potato cassava, quinoa and green reveals a shortage of vitamins D and B12, cholesterol and sodium salt. Since vitamin D deficiency results in demineralization of bone. Vitamin B12 is essential to prevent pernicious anemia.Fish contains both vitamins D and B12. The pupa of the silkworm becomes the impor-tant nourishment source as protein and lipid. The silk thread uses it as clothing and cosmetics and medical supplies. However, we can use the silk thread as food as protein after finish to use. A law of nature shakes high quality oils and fats included in termite for cooking. I use the bee as food after having used it for the pollination of the plant. Of course the honey becomes the important food, too. The snail and mud snail become the food as protein. We decided to use the menu consisting of the basic vegetarian menu plus insect and loach for further conceptual design of space agriculture. We succeeded to develop joyful and nutritious space recipe at the end. Because of less need of agricultural resources at choosing ecological members from the lower ladder of the food chain, our space recipe could be a proposal to solve the food problem on Earth.In addition, as for these space foods, a save is possible by refrigeration for 20 days.
Fish ladder of Lajeado Dam: migrations on one-way routes?
Abstract in portuguese Escadas de peixes são geralmente concebidas para restabelecer a conectividade entre habitats críticos de espécies migradoras, atenuando os impactos da interceptação de rotas de migração por barragens. Para que esse instrumento de manejo tenha significado na conservação das espécies é necessário que a permeabilidade dessa conexão seja ampla e que os movimentos ascendentes e descendentes sejam assegurados. Entretanto, como os reservatórios têm uma hidrodinâm (more) ica muito distinta do rio original, postula-se que, pelo menos em sua área mais interna ele possa se constituir em uma barreira adicional a esses movimentos, especialmente os descendentes. Assim, esse trabalho busca avaliar se peixes migradores e sua prole dispersam para os trechos abaixo da barragem após a transporem e desovar nos trechos altos da bacia. Com esse propósito, foi avaliado o grau de restrição oferecido pelas áreas lênticas de reservatórios aos deslocamentos descendentes de ovos, larvas e adultos de espécies migradoras, identificada a abundância e composição das larvas presentes no plâncton das imediações da barragem e comparada a intensidade dos movimentos ascendentes e descendentes de peixes. Foram realizadas amostragens de ictioplâncton a montante, no reservatório e em seu trecho a jusante, bem como nas imediações da barragem de Lajeado (rio Tocantins; UHE Luis Eduardo Magalhães) durante o período de outubro de 1999 a setembro de 2004 e determinado, experimentalmente, em oito ocasiões durante o período de junho de 2004 e março de 2005, a densidade de peixes em movimentos ascendentes e descendentes na escada de peixes desse reservatório. Já a avaliação na distribuição de peixes migradores em reservatórios foi baseada no acompanhamento dos desembarques da pesca comercial praticada ao longo do reservatório de Itaipu durante os quatro anos que precederam a construção do mecanismo de transposição atualmente existente (2001 a 2003). Os resultados obtidos demonstraram que os ovos e larvas de peixes em deriva, pelo rio Tocantins, desaparecem das amostras na metade inferior do reservatório e aqueles encontrados na água vertida ou turbinada pela barragem de Lajeado e na escada de peixes pertencem, essencialmente, a clupeídeos não migradores, com desovas nas áreas internas do reservatório. Revelam ainda que, em reservatório sem mecanismo de transposição, os peixes migradores selecionam habitats que ainda mantém características fluviais, nos segmentos superiores da área represada. Os experimentos realizados na escada revelam que os movimentos descendentes são irrelevantes quando comparados aos ascendentes. Conclui-se, portanto, que a escada de peixes de Lajeado, e possivelmente de várias outras barragens, é essencialmente uma via de mão única que promove subidas massivas de migrantes sem o almejado retorno de adultos ou de sua prole. Assim, a baixa permeabilidade das conexões proporcionadas por esses instrumentos de manejo pode aumentar drasticamente o nível do impacto ambiental para cuja atenuação ele foi concebido. Abstract in english Fish ladders are generally conceived to reestablish connectivity among critical habitats for migratory species, thus mitigating the impacts of the blockage of migration routes by dams. If this management tool is to be meaningful for conserving fish species, it must provide a fully permeable connection and assure both upward and downward movements. However, because reservoirs have very different hydrodynamics than the original river, it is expected that, at least in the in (more) ner area, they may constitute an additional barrier to this movement, especially for descending fish. Thus, the present study sought to determine if migratory fish and their offspring disperse downstream from the dam after ascending a ladder and spawning in the upper reaches of a basin. To achieve this purpose, we evaluated the limitation imposed by lentic areas to the descent of eggs, larvae and adults of migratory species; we also determined the abundance and composition of larvae present in the plankton near the dam, and compared the intensity of the upward and downward movements of adult fish. Samples of ichthyoplankton were taken upriver, inside the reservoir, in the river downstream from the dam, and in the forebay of the Lajeado Dam on the Tocantins River (Luis Eduardo Magalhães Hydroelectric Plant), from October, 1999 through September, 2004. The densities of fish ascending and descending the ladder were determined experimentally on eight occasions, from June, 2004 to March, 2005. Due to difficulties in identifying the true fish origin (up or down) in the environments connected by the fish passage system, the evaluation of the distribution of migratory fish in reservoirs was based on the landings of the commercial fishery conducted along the Itaipu Reservoir during the four years preceding (2001 through 2003) the construction of the lateral channel (fish-passage mechanism). Fish eggs and larvae drifting down the Tocantins River did not appear in samples taken in the lower half of the reservoir; those found in water flowing through the spillways, turbines or fish ladder of Lajeado Dam belonged essentially to non-migratory clupeids that spawn in the inner part of the reservoir. In addition, results showed that in a reservoir with no fish-passage mechanism, migrants select habitats that still maintain riverine characteristics, in the upper parts of the impounded area. The downward movements are negligible compared to those upward, in the experiments conducted in the fish ladder. It is concluded, therefore, that the Lajeado fish ladder, and possibly those at other dams, is essentially a one-way route that promotes upstream movements of migrants, without the necessary return of adults or their offspring. Thus, the low permeability of the connections provided by these management tools can drastically increase the level of environmental impact that they were actually intended to reduce.
Migration patterns of adult spring chinook salmon above Willamette Falls differed depending on when the fish passed the Falls, with considerable among-fish variability. Early-run fish often terminated their migration for extended periods of time, in association with increased flows and decreased temperatures. Mid-run fish tended to migrate steadily upstream at a rate of 30-40 km/day. Late-run fish frequently ceased migrating or fell back downstream after migrating 10-200 km up the Willamette River or its tributaries; this appeared to be associated with warming water during summer and resulted in considerable mortality. Up to 40% of the adult salmon entering the Willamette River System above Willamette Falls (i.e. counted at the ladder) may die before reaching upriver spawning areas. Up to 10% of the fish passing up over Willamette Falls may fall-back below the Falls; some migrate to the Columbia River or lower Willamette River tributaries. If rearing conditions at hatcheries affect timing of adult returns because of different juvenile development rates and improper timing of smolt releases, then differential mortality in the freshwater segment of the adult migrations may confound interpretation of studies evaluating rearing practices.
This study examines envirorunental mitigation practices that provide upstream and downstream fish passage and protection at hydroelectric projects. The study includes a survey of fish passage and protection mitigation practices at 1,825 hydroelectric plants regulated by the Federal Energy Regulatory Commission (FERC) to determine frequencies of occurrence, temporal trends, and regional practices based on FERC regions. The study also describes, in general terms, the fish passage/protection mitigation costs at 50 non-Federal hydroelectric projects. Sixteen case studies are used to examine in detail the benefits and costs of fish passage and protection. The 16 case studies include 15 FERC licensed or exempted hydroelectric projects and one Federally-owned and-operated hydroelectric project. The 16 hydroelectric projects are located in 12 states and range in capacity from 400 kilowatts to 840 megawatts. The fish passage and protection mitigation methods at the case studies include fish ladders and lifts, an Eicher screen, spill flows, airburst-cleaned inclined and cylindrical wedgewire screens, vertical barrier screens, and submerged traveling screens. The costs, benefits, monitoring methods, and operating characteristics of these and other mitigation methods used at the 16 case studies are examined.
DISPOSAL OF SMALL-SCALE FISH PROCESSING WASTE THROUGH COMPOSTING
Large catfish processors in the U.S. typically recycle fish waste into fish meal. For small-scale processors or aquaculture research facilities, fish waste disposal can be problematic. We adapted a design developed for composting wastes from Minnesota fishing lodges and tested it for suitability fo...
Deterministic Chaos: A signature of Quantumlike Mechanics in Self-Organized Adaptive Network
The observed long-range spatiotemporal correlations of real world dynamical systems is governed by quantumlike mechanics with inherent non-local connections. In summary, microscopic scale local fluctuations form a unified self-organized adaptive network manifested as the macro-scale dynamical system with implicit ordered energy flow between the larger and smaller scales. Such a concept of ladder networks may find applications in the design of artificial intelligence systems.
This paper presents an analytical procedure which enables a quick and, at the design stage, correct evaluation of the ground fault current distribution, for a fault in a substation supplied by a line composed of two or more mutually different (overhead and underground) sections. The advantages of the method are based on the simplicity and accuracy of the formulae for solving uniform lumped parameter ladder circuits of any size and under any terminal conditions. Copyright Copyright 2006 John Wiley & Sons, Ltd.
Purpose - The purpose of this paper is to explore women's perceptions about the benefits and risks of fish consumption, while exploring differences on their views about wild and farmed fish, considering light fish consumers. Design/methodology/approach - The methodology adopted is exploratory, using focus group technique, segregating women by education level (higher education versus lower education). A focus group guide was designed, taking into account the following dimensions: attitudes towards fish consumption and perceptions towards farmed fish relative to wild fish, also considering risk perceptions related to farmed versus wild fish. Findings - This study has shown that fish consumers enjoy the taste of fish and they are strongly convinced that eating fish is healthy. The main reason...
Historical records indicate that sockeye salmon were once found in most of the lakes in the Okanagan River Basin. Currently, the only sockeye population within the Okanagan River Basin is found in Osoyoos Lake. Abundance of this stock has declined significantly in the last fifty years. The Okanagan Nation and tribes in the U.S. have proposed re-introducing the species into Okanagan Lake, which has a large rearing capacity. However, assessing the potential benefits and risks associated with a reintroduction of sockeye salmon into Okanagan Lake is difficult because of uncertainties about factors that determine production of Okanagan sockeye, and potential interactions with other species in Okanagan Lake. Associated with this proposal are the potential risks of re-introduction of sockeye salmon into Okanagan Lake. One of these is the effects of sockeye on the resident Okanagan Lake kokanee population, which has declined significantly in the past several years because of habitat loss due to human encroachment, competition with introduced mysid shrimp, and the reduction of biological productivity in the lake as municipalities have moved to more complete effluent treatment. Another concern is the possibility of the transmission of diseases that are currently not found in Okanagan and Skaha lakes from re-introduced sockeye to resident fish. An additional concern is the risk that exotic species (e.g. tench, largemouth bass), that have become established in southern Okanagan Lakes (principally as a result of purposeful introductions in the US Columbia/Okanagan river system), may be able to extend their range to Skaha and Okanagan Lakes, through fish ladders provided at the outlets of Vaseaux (McIntyre Dam) and Skaha Lakes (Okanagan Falls Dam), for natural upstream migration of sockeye. A transboundary multi-agency workshop was hosted in November of 1997 to discuss the potential risks and benefits of reintroducing sockeye salmon into Okanagan Lake. These discussions were summarized into a Draft Action Plan that recommended that sockeye be re-introduced to Skaha Lake as an experimental management strategy to resolve some of these uncertainties (Peters et al. 1998). The purpose of this project is to assess the risks and benefits of an experimental reintroduction of sockeye salmon into Skaha Lake. The assessment will be accomplished by completing the following six objectives over three years: (1) Disease Risk Assessment; (2) Exotic species Re-introduction risk Assessment; (3) Inventory of Existing Habitat and Opportunities for Habitat Enhancement; (4) Development of a life-cycle model of Okanagan salmonids, including interaction with resident kokanee; (5) Development of an experimental design and; (6) Finalize a plan for experimental re-introduction of sockeye salmon into Skaha Lake and associated monitoring programs.
Pacific Northwest National Laboratory (PNNL) evaluated two fish screen facilities in the Walla Walla River basin in 2005 and early 2006. The Garden City/Lowden screen site was evaluated in April and June 2005 to determine whether the fish screens met National Marine Fisheries Service criteria to provide safe passage for juvenile salmonids. Louvers behind the screens at the Nursery Bridge Fishway were modified in fall 2005 in an attempt to minimize high approach velocities. PNNL evaluated the effects of those modifications in March 2006. Results of the Garden City/Lowden evaluations indicate the site performs well at varying river levels and canal flows. Approach velocities did not exceed 0.4 feet per second (fps) at any time. Sweep velocities increased toward the fish ladder in March but not in June. The air-burst mechanism appears to keep large debris off the screens, although it does not prevent algae and periphyton from growing on the screen face, especially near the bottom of the screens. At Nursery Bridge, results indicate all the approach velocities were below 0.4 fps under the moderate river levels and operational conditions encountered on March 7, 2006. Sweep did not consistently increase toward the fish ladder, but the site generally met the criteria for safe passage of juvenile salmonids. Modifications to the louvers seem to allow more control over the amount of water moving through the screens. We will measure approach velocities when river levels are higher to determine whether the louver modifications can help correct excessive approach velocities under a range of river levels and auxiliary water supply flows.
A robust embedded ladder-oxide (k=2.9)/copper (Cu) multilevel interconnect is demonstrated for 0.13 ?m complementary metal oxide semiconductor (CMOS) generation. A stable ladder-oxide intermetal dielectric (IMD) is integrated by the Cu metallization with a minimum wiring pitch of 0.34 ?m, and a single damascene (S/D) Cu-plug structure is applied. An 18% reduction in wiring capacitance is obtained compared with that in SiO2 IMDs. The superior controllability of metal thickness by the S/D process enables us to enhance the MPU maximum frequency easily. The stress-migration lifetime of vias on wide metals for the S/D Cu-plug structure is longer than that for a dual damascene (D/D) structure. Reliability test results such as electromigration (EM), the temperature dependant dielectric breakdown (TDDB) of Cu interconnects, and pressure cooker test (PCT) results are acceptable. Moreover, a high flexibility in a thermal design is obtained.
Synthesis of ladder-like polynorbornenes with n-type perylenendiimide derivatives as bridges
Abstract Polymerizable tetrachloro-perylenediimdes containing endo/exo-norbornene groups on both imide sides were designed and synthesized. Endo/Exo-type soluble ladder-like polynorbornenes with perylenediimide (PDI) as bridges were prepared by ring-opening metathesis polymerization (ROMP). XRD characterizations showed that the ladder-like polynorbornenes had ordered structures similar to the supramolecular precursors assembled from the corresponding monomers. TGA measurements demonstrated great thermal stabilities for the both target P1-Endo and P2-Exo with Td of about 320 C at 5 wt % loss, respectively, which is important for further application in devices. Both polymers have good solubility in common organic solvents and easy to form thin films. Photophysical studies and cyclic voltamme...
A new method of calculating input impedances of inhomogeneous ladder networks
We describe how to calculate the input impedances of finite inhomogeneous ladder networks by transforming a product of 2 × 2 transfer matrices to a product of diagonal matrices up to a prefactor and a postfactor. In particular, we find the input impedances in closed form for three inhomogeneous ladder networks (a tapered ladder network, a balanced composite right/left-handed ladder network, and a ladder network with an arbitrary period) with a periodic transfer matrix.
Are fish eaters healthier and do they consume less health-care resources?
Objective: Regular dietary intake of fish is associated with reduced risk of developing cardiovascular and other chronic diseases, and may improve general well-being. If fish eaters are healthier, they may use fewer health-care resources. The present study aimed to describe the reported intake of fish and fish products in a Danish general population, and to investigate whether fish consumption is associated with generic measures of self-reported health and consumption of health-care resources. Design: Data on eating patterns and health status for 3422 Danish adults were obtained by telephone interview in the Funen County Health Survey. These data were merged with individual-level register data on health-care utilisation. Survey respondents were categorised into those consuming fish at least once weekly (fish eaters) and those consuming fish less frequently (non-fish eaters). Results: People who reported eating fish twice monthly or once weekly had significantly better overall self-reported health than thosewho rarely eat fish, even after adjustment for age, gender, social characteristics and lifestyle factors. Fish eaters did not have significantly lower aggregated health-care costs, although their hospital utilisation was significantly lower than that for non-fish eaters. Conclusions: Moderate fish consumption was associated with better self-reported general health even after controlling for possible confounding variables. Overall, fish eaters appeared to use the same amount of health-care resources as non-fish eaters, although fish eaters used more medicine but were less likely to be admitted to a hospital.
Molecular Mechanism of Thioflavin-T Binding to the Surface of [beta]-Rich Peptide Self-Assemblies
A number of small organic molecules have been developed that bind to amyloid fibrils, a subset of which also inhibit fibrillization. Among these, the benzothiol dye Thioflavin-T (ThT) has been used for decades in the diagnosis of protein-misfolding diseases and in kinetic studies of self-assembly (fibrillization). Despite its importance, efforts to characterize the ThT-binding mechanism at the atomic level have been hampered by the inherent insolubility and heterogeneity of peptide self-assemblies. To overcome these challenges, we have developed a minimalist approach to designing a ThT-binding site in a 'peptide self-assembly mimic' (PSAM) scaffold. PSAMs are engineered water-soluble proteins that mimic a segment of beta-rich peptide self-assembly, and they are amenable to standard biophysical techniques and systematic mutagenesis. The PSAM beta-sheet contains rows of repetitive amino acid patterns running perpendicular to the strands (cross-strand ladders) that represent a ubiquitous structural feature of fibril-like surfaces. We successfully designed a ThT-binding site that recapitulates the hallmarks of ThT-fibril interactions by constructing a cross-strand ladder consisting of contiguous tyrosines. The X-ray crystal structures suggest that ThT interacts with the beta-sheet by docking onto surfaces formed by a single tyrosine ladder, rather than in the space between adjacent ladders. Systematic mutagenesis further demonstrated that tyrosine surfaces across four or more beta-strands formed the minimal binding site for ThT. Our work thus provides structural insights into how this widely used dye recognizes a prominent subset of peptide self-assemblies, and proposes a strategy to elucidate the mechanisms of fibril-ligand interactions.
One-way data transfer for PLC to VME status reporting at the Advanced Photon Source
The Personnel Safety System for the experimental beamlines at the Advanced Photon Source will use a large number of Allen Bradley Programmable Logic Controllers (PLC) to replace conventional relay logic. PLCs allow for the design of a very advanced safety system that can handle a large number of I/O points. Certain situations Require monitoring of the safety system from various locations around the storage ring via EPICS OPI (operator interface)consoles. This presentation covers the method of choice for transferring data from the Personnel Safety System into an EPICS database. Specifics on PLC ladder design, EPICS database design, and hardware selection are also discussed.
Hood River and Pelton Ladder Evaluation Studies : Annual Report 1994.
In 1992, the Northwest Power Planning Council approved the Hood River and Pelton ladder master plans within the framework of the Columbia River Basin Fish and Wildlife Program. The master plans define an approach for implementing a hatchery supplementation program in the Hood River subbasin. The hatchery program as defined in the master plans is called the Hood River Hatchery Production Program (HRPP). The HRPP will be phased in over several years and will be jointly implemented by the Oregon Department of Fish and Wildlife (ODFW) and the Confederated Tribes of the Warm Springs (CTWS) Reservation. In December 1991, a monitoring and evaluation program was implemented in the Hood River subbasin to collect life history and production information on stocks of anadromous salmonids returning to the Hood River subbasin. The program was implemented to provide the baseline information needed to: (1) evaluate various management options for implementing the HRPP and (2) determine any post-project impacts the HRPP has on indigenous populations of resident fish. Information collected during the 1992-94 fiscal years will also be used to prepare an environmental impact statement (EIS) evaluating the program`s impact on the human environment. To begin construction on project facilities, it was proposed that the HRPP be implemented in two phases. Phase I would include work that would fall under a {open_quotes}categorical exclusion{close_quotes} from NEPA, and Phase II would include work requiring an EIS prior to implementation. This report summarizes the life history and escapement data collected in the Hood River subbasin and the status work of implemented under Phase I of the HR Life history and escapement data will be used to: (1) test the assumptions on which harvest and escapement goals for the Hood River and Pelton ladder master plans are based and (2) develop biologically based management recommendations for implementing the HRPP.
Modeling species-genes data for efficient phylogenetic inference.
In recent years, biclique methods have been proposed to construct phylogenetic trees. One of the key steps of these methods is to find complete sub-matrices (without missing entries) from a species-genes data matrix. To enumerate all complete sub-matrices, (17) described an exact algorithm, whose running time is exponential. Furthermore, it generates a large number of complete sub-matrices, many of which may not be used for tree reconstruction. Further investigating and understanding the characteristics of species-genes data may be helpful for discovering complete sub-matrices. Therefore, in this paper, we focus on quantitatively studying and understanding the characteristics of species-genes data, which can be used to guide new algorithm design for efficient phylogenetic inference. In this paper, a mathematical model is constructed to simulate the real species-genes data. The results indicate that sequence-availability probability distributions follow power law, which leads to the skewness and sparseness of the real species-genes data. Moreover, a special structure, called "ladder structure", is discovered in the real species-genes data. This ladder structure is used to identify complete sub-matrices, and more importantly, to reveal overlapping relationships among complete sub-matrices. To discover the distinct ladder structure in real species-genes data, we propose an efficient evolutionary dynamical system, called "generalized replicator dynamics". Two species-genes data sets from green plants are used to illustrate the effectiveness of our model. Empirical study has shown that our model is effective and efficient in understanding species-genes data for phylogenetic inference. PMID:17951845
Simulations of The Dalles Dam Proposed Full Length Spillwall
This report presents results of a computational fluid dynamics (CFD) modeling study to evaluatethe impacts of a full-length spillwall at The Dalles Dam. The full-length spillwall is being designed and evaluated as a structural means to improve tailrace egress and thus survival of juvenile fish passing through the spillway. During the course of this study, a full-length spillwall at Bays 6/7 and 8/9 were considered. The U.S. Army Corps of Engineers (USACE) has proposed extending the spillwall constructed in the stilling basin between spillway Bays 6 and 7 about 590 ft farther downstream. It is believed that the extension of the spillwall will improve egress conditions for downstream juvenile salmonids by moving them more rapidly into the thalweg of the river hence reducing their exposure to predators. A numerical model was created, validated, and applied the The Dalles Dam tailrace. The models were designed to assess impacts to flow, tailrace egress, navigation, and adult salmon passage of a proposed spill wall extension. The more extensive model validation undertaken in this study greatly improved our confidence in the numerical model to represent the flow conditions in The Dalles tailrace. This study used these validated CFD models to simulate the potential impacts of a spillwall extension for The Dalles Dam tailrace for two locations. We determined the following: (1)The construction of an extended wall (between Bays 6/7) will not adversely impact entering or exiting the navigation lock. Impact should be less if a wall were constructed between Bays 8/9. (2)The construction of a wall between Bays 6/7 will increase the water surface elevation between the wall and the Washington shore. Although the increased water surface elevation would be beneficial to adult upstream migrants in that it decreases velocities on the approach to the adult ladder, the increased flow depth would enhance dissolved gas production, impacting potential operations of the project because of water quality. A wall between Bays 8/9 should have a lesser impact as the confined spill would be across more bays and the relative flow constriction less. (3) The 405 kcfs case was used for the rapid assessment of flow conditions and hydraulic mechanisms that might be responsible for the unexpected erosion at the end of the shelf downstream of Bay 7.
The spermatogenesis-associated 17 gene (SPATA17, previously named MSRG-11) was reported to be a candidate spermatocyte apoptosis-related gene which may play a critical role in human spermatogenesis, especially in meiosis. Analysis of SPATA17 expression and regulation in zebra fish may provide insight into the understanding of the complicated process of gonadogenesis and its potential function in spermatocyte cell apoptosis. In this study, we cloned and characterized the SPATA17 gene from zebra fish which consists of nine exons separated by eight introns. The consensus open reading frame (1258 bp) encodes a polypeptide of 357 amino acids which shares 44% identity with the human SPATA17 gene. Bioinformatic analysis reveals that SPATA17 protein contains three short calmodulin-binding motifs (IQ motif) and is considered to play a critical role in interactions with CaM proteins. Multi-tissue RT-PCR and Northern blot results demonstrated that the zebra fish SPATA17 gene was expressed strongly in testis and a slight amount of expression in ovary. Flow cytometry analysis and genomic DNA ladders result showed that the expression of SPATA17 protein in the GC-1 cell line could accelerate cell apoptosis. Analysis of the SPATA17 sequence and its spatial expression pattern indicate that this gene is highly conserved and may play an important role in the process of zebra fish gonadogenesis. PMID:21108043
Adult Fishway Inspections on the Columbia and Snake Rivers, 1984-1985 Annual Report.
Mainstream projects which have adult fish passage facilities are inspected by project operators, fishway attendants, and state and federal fishery agencies. The overall movement of upstream migrants in 1984 appeared to be satisfactory with few delays. Special efforts were made by fishery agencies and Corps personnel to check on potential problems which appeared to exist at The Dalles and John Day Dams this year. However, adult passage facilities were operating ''in criteria'' and fish may have been delayed by temperature or other factors. Inspections were generally made once a month. Some projects were operating at less than full criteria as seen during inspections by fishery agencies this year. It appears that during periods of low tailwater, certain projects have difficulty maintaining proper head at main fishway entrances. Some main entrance gates bottom-out and water depth over these weirs are not up to desired criteria. Also it was noted that auxiliary water pumps were not being run at a rate to achieve the desired amount of water for attracting fish to the fish ladders and maintaining proper head at main fishway entrances.
Ultrafast energy transfer in blended polyphenothiazine/polyphenylene vinylene film
Two-polymers, phenothiazine derivatives (PQ) and poly 2-methoxy-5-(2prime-ethylhexyloxy)-1,4-phenylene vinylene (MEH-PPV), who show complementary absorption to the solar spectrum are blended for broader absorption. The energy transfer from PQ to MEH-PPV is designed and observed at ultrafast time scale of 2ps. Foster resonance energy transfer (FRET) process is regarded as the mechanism. Band gap of PQ is determined as 2.5eV. With this design, a ladder type energy transfer and collection is suggested.
Ultrafast energy transfer in blended polyphenothiazine/polyphenylene vinylene film
Two-polymers, phenothiazine derivatives (PQ) and poly 2-methoxy-5-(2?-ethylhexyloxy)-1,4-phenylene vinylene (MEH-PPV), who show complementary absorption to the solar spectrum are blended for broader absorption. The energy transfer from PQ to MEH-PPV is designed and observed at ultrafast time scale of ˜2 ps. Föster resonance energy transfer (FRET) process is regarded as the mechanism. Band gap of PQ is determined as 2.5 eV. With this design, a ladder type energy transfer and collection is suggested.
The purpose of this study is to assess the design and operation of rotary drum screens. Chinook salmon Oncorhynchus tshawytscha and steelhead O. mykiss are the potentially affected fish. Cold-branded fish are released upstream of the screen facilities. For descaling tests, the fish are captured as they exit the facility and are examined for injuries, descaling, and post-test mortalities. For screen passage tests, nets are placed in the irrigation ditch, downstream of the screen facilities, to determine if fish can pass through or over the screens. More than 100 tests have been conducted with almost 35,000 fish. Additionally, nearly 2000 native fish have been evaluated. Usually less than 2% of the test fish are injured or dead, and the condition of test fish does not differ from the controls. Less than 2% of the fish pass through or over the screens when the screen seals are properly installed and maintained.
Evaluate Habitat Use and Population Dynamics of Lampreys in Cedar Creek, 2000 Annual Report.
Pacific lamprey (Lampetra tridentata) in the Columbia River Basin have declined to a remnant of their pre-1940s populations and the status of the western brook lamprey (L. richardsoni) is unknown. Identifying the biological and ecological factors limiting lamprey populations is critical to their recovery, but little research has been conducted on these species within the Columbia River Basin. This ongoing, multi-year study examines lamprey populations in Cedar Creek, Washington, a third-order tributary to the Lewis River. Adult (n = 40), metamorphosed (n = 116), transforming (n = 10), and ammocoete (n = 870) stages from both species were examined in 2000. Lamprey were captured using adult fish ladders, rotary screw traps, and lamprey electrofishers, and spawning ground surveys were conducted. US Forest Service level II and strategic point-specific habitat surveys were conducted to assess habitat requirements of both adult and larval lamprey. Multivariate statistics will be applied to determine relationships between abundance and habitat.
White Sturgeon Passage at The Dalles Dam
Researchers at the USGS Western Fisheries Research Center's Columbia River Research Laboratory, working with the U.S. Army Corps of Engineers, sought to better understand upstream and downstream passage of white sturgeon at dams. A study at The Dalles Dam provided the opportunity to compare two fish ladders; one that passes sturgeon upstream to one that does not, to determine if subtle differences in construction result in better passage of white sturgeon. Researchers conducted a study using a combination of acoustic and radio telemetry technologies to obtain information on juvenile and adult white sturgeon near The Dalles Dam, with the objectives of characterizing the distribution and movements of white sturgeon in the immediate vicinity of the dam and to determine timing and routes of upstream and downstream passage.
A ‘Hybrid’ recirculating aquaculture system design utilizes elements of both a ‘Centralized’ design concept with a single water treatment system for a number of fish tanks and the ‘Modular’ design concept which employs a individual treatment system for each fish culture tank. The ‘Hybrid’ recirculat...
Yakima River Spring Chinook Enhancement Study, 1987 Annual Report.
The smelt outmigration was monitored at wapatox on the Naches River and Prosser on the lower Yakima. The spring outmigration at Wapatox was estimated to be 16,141 smolts. The 1987 spring outmigration of wild spring chinook from the Yakima Basin was estimated to be 251,975 smolts at Prosser. The survival from egg to smelt was calculated using the 1985 redd counts and the 1987 smolt outmigration at Prosser. The estimated survival was 4.16%, which gives a mean egg to smolt survival over four years of 6.32%. In 1987 a total of 3,683 adult and 335 jack spring chinook salmon returning to the Yakima River were counted at Prosser fish ladder. This gives a total of 4,018 salmon returning to Prosser Dam. The median dates of passage were May 12 and May 16 for adults and jacks respectively. An additional 372 fish were estimated to have been caught in the Yakima River subsistence dipnet fishery below Horn Rapids and Prosser Dams. Therefore, total return to the Yakima system was 4,390 spring chinook salmon. Spring chinook were counted at Roza Dam from May 1 to September 30, 1987. Passage at Roza Dam was 1,610 adult and 67 jack spring chinook for a total of 1,677 wild fish. The median dates of passage at Roza Dam were May 29 and May 26 for spring chinook adults and jacks respectively. The smolt to adult (S{sub sa}) survival was calculated based on the 1983 smelt outmigration estimated at Prosser and the 1984 return of jacks (3 year old fish) the 1985 return of four year old adults, and the 1986 return of five year old fish to the Yakima River. It was estimated that 6,012 wild three, four, and five year old fish returned from an estimated smolt outmigration of 135,548 fish in 1983. This gives an estimated survival from smolt to adult of 4.4%. The smolt to adult survival for the 1984 smolt outmigration was 5.3% with 423 jacks returning in 1985, 5,163 four year old adults returning in 1986, and 983 five year old fish returning in 1987 fran an estimated 123,732 smolts in 1984. Spring chinook adults from fourteen different hatchery release groups were recovered in 1987. A total of 211 coded wire tags were recovered and these were expanded to an estimated 253 returning hatchery fish in 1987. Nine of these fish were jacks.
In the first part of this paper we introduce the category of ladders and develop the underlying theory. A ladder consists of a sequence of vector spaces (V n ) and linear operators Formula Not Shown acting between these vector spaces in ascending and descending direction. Unlike as in classical quantum mechanics ladders are defined as objects of a category, the corresponding notion of ladder homomorphisms allows to perform a mathematically structural and rigorous analysis of ladder theory. We allow dependence on n for spaces and operators in the ladder. The job in ladder theory is to find SIE-subladders, on which the Intrinsic Endomorphisms Formula Not Shown and Formula Not Shown act as Scalars ? n . A fundamental ladder theorem will provide conditions on the (generalized) commutators or a...
Laboratory Studies on the Effects of Shear on Fish
The overall objective of our studies was to specify an index describing the hydraulic force that fish experience when subjected to a shear environment. Fluid shear is a phenomenon that is important to fish. However, elevated levels of shear may result in strain rates that injure or kill fish. At hydroelectric generating facilities, concerns have been expressed that strain rates associated with passage through turbines, spillways, and fish bypass systems may adversely affect migrating fish. Development of fish friendly hydroelectric turbines requires knowledge of the physical forces (injury mechanisms) that impact entrained fish and the fish's tolerance to these forces. It requires up-front, pre-design specifications for the environmental conditions that occur within the turbine system, in other words, determining or assuming that those conditions known to injure fish will provide the descriptions of conditions that engineers must consider in the design of a turbine system. These biological specifications must be carefully and thoroughly documented throughout the design of a fish friendly turbine. To address the development of biological specifications, we designed and built a test facility where juvenile fish could be subjected to a range of shear environments and quantified their biological response.
Catching and eating fish is usually viewed as a fun, healthy and safe activity. However, with continuing increases in fish consumption advisories due to the contamination of our environment, anglers have to decide whether or not to eat the fish they catch. The Clinch River arm of Watts Bar Reservoir is under a fish consumption advisory because of elevated PCB concentrations in striped bass (Morone saxatilis), catfish (Ictalurus spp.) and sauger (Stizostedion canadense) due in part from contaminants released from the US Department of Energy's (USDOE's) Oak Ridge Reservation (ORR) in East Tennessee. To obtain information about the demographics, fishing behavior, knowledge, fish consumption and risk perception of anglers, a survey was conducted of 202 people actively fishing either on land or by boat along the Clinch River arm of Watts Bar Reservoir adjacent to the ORR from Melton Hill Dam to the Poplar Creek confluence or on Poplar Creek within ORR boundaries from mid-March to early November 2001. Even though 81% of people interviewed knew about the fish consumption advisories for the study area, 48% of them thought the fish were safe to eat, while 38% ate the fish that they caught from the study area. Approximately 36% of anglers who had knowledge of the fish consumption warnings ate fish from the study area. Providing confirmation that people fish for many reasons, 35% of anglers interviewed did not eat fish at all. The majority of anglers interviewed knew about the fish consumption advisories because of the signs posted throughout the study area. However, few people knew the correct fish advisories. Significantly fewer blacks had knowledge of the fish consumption warnings than whites. Information resulting from this study could be used to design a program with the objective of reaching the people who may be most at risk from eating fish caught from the Clinch River arm of Watts Bar Reservoir.
An adult Pacific lamprey (Lampetra tridentata) escapement estimate was generated in the lower Deschutes River during run year 2008. This included a mark-recapture study to determine adult abundance and a tribal subsistence creel. Fish measuring less than 10.5 cm received two marks for the mark-recapture estimate while those measuring greater than 10.5 cm were surgically implanted with radio transmitters to monitor migration upstream of Sherars Falls (rkm 70.4). Radio telemetry was used to determine habitat, focal spawning areas and spawn timing. All fish were collected at the Sherars Falls fish ladder from July-October 2008 using a long handled dip-net. Escapement was generated using a two event mark-recapture experiment. Adult lamprey populations were estimated at 3,471 (95% CI = 2,384-5,041; M = 101; C = 885 R = 25) using Chapman's modification of the Peterson estimate. The relative precision around the estimate was 31.42. Tribal harvest was approximately 806 adult lamprey (95% CI = +/- 74) with a total escapement of 2,669. Fourteen lamprey received radio tags and were released at Lower Blue Hole recreation site (rkm 77.3). Movement was recorded by mobile, fixed site and aerial telemetry methods. Upstream movements of lamprey were documented from July through December 2008 with most lamprey over-wintering in the mainstem Deschutes River.
Stocking strategy for the rehabilitation of a regulated brown trout (Salmo trutta L. ) river
Regulation of the catchment area of the Norwegian river Gudbrandsdalslaagen began in 1919. The lowermost power station on the main river was completed in 1964 and is situated about 10 km above the large Norwegian lake, Mjoesa. The lake is the foraging area of the Hunderstrain of brown trout, the fastest growing of all Norwegian trout. The running of the power plant has led to a severe reduction in water flows below the dam, and the most important spawning and nursery areas of the Hunder strain has been affected. The natural smolt production has been permanently reduced. The rehabilitation programme has included the construction of a fish ladder through the dam and a fixed minimum flow. A hatchery was built and a stocking programme using the local strain was implemented. The effect of stocking has been the easiest of the relief measures to evaluate. Hatchery reared fish constitute a growing share of the spawning population. During the last three years their share has been close to 60%. Reared fish constitute 30-40% of the trout caught in Lake Mjoesa. The average and best returns of tagged groups have been 25 and 50%, respectively, but return rates are highly dependent on release length and time and place of stocking. (Author)
The eel pollutant monitoring network in Flanders, Belgium. Results of 10 years monitoring
Since 1994 the Institute for Forestry and Game Management (IBW) has build out a pollutant monitoring network for public water bodies in Flanders (Belgium) using eel (Anguilla anguilla) as a biomonitor. The results presented here come from 2000 individually analysed eels originating from 260 different localities in Flanders, including rivers, canals, polder waters and closed water bodies. The sampling took place from 1994 to 2002. Eel is used for biomonitoring because it is a very fatty (strong lipophylic character of a.o. pesticides and PCB's), benthic, sedentary (during the yellow eel phase), long-living and widespread fish (in non-polluted as well as in polluted waters) and because of the absence of a seasonal effect through reproduction and its place on the trophic ladder. Some results represented are: (1) on 80 % of all sampled localities the Belgian PCB-standard for fish (75ng/g BW) is exceeded; (2) in the north-western part of Flanders is a severe lindanecontamination; (3) many long time prohibited pesticides are still found in considerable amounts in our food-chain; (4) several locations contain high to very high concentrations of brominated flame retardants. For several contaminants a comparison is made between the Flemish values and other values found in literature. The results of the Flemish eel pollutant monitoring network have initiated a catch-and-release obligation for eel in Flanders and for all fish on the 5 most polluted waters in Flanders.
An ultra-wide-band 3.1-10.6GHz LNA design in 0.18mm SiGe BiCMOS
A two-stage monolithic ultra-wide-band (UWB) low-noise-amplifier (LNA) designed for MB-OFDM in 0.18mm SiGe BiCMOS process is presented. With an optimized configuration combining advantages of RES-feedback and LC-ladder matching structure, the adjustable wide input matching is got and noise figure (NF) is controlled to a relevant low status. The measured S21 is from 7.6 to 14.2dB over the 3-11GHz operating band, NF is from 3.2dB to 4.8dB. With a 2.5V power supply, the LNA has an overall power consumption of 14.5mW.
A precisely gain controlled RF front end for T-DMB tuner ICs
A precisely gain controlled RF front end is developed for T-DMB tuner IC applications. A precise RF gain control with 50dB dynamic range is achieved in RF VGA by employing a capacitive ladder type attenuator. The proposed method enables us to design and analyze very fine gain step VGA concretely. Implemented in 0.13µm CMOS, the tuner RF front end consumes 9.7mA at 1.2V supply. Measured results are NF < 1.5dB at the maximum gain and the accurate 1dB gain step is obtained.
This is the Spanish translation of a "how-to" manual, designed as a working and teaching tool for extension agents as they establish and/or maintain local fish pond operations. The manual presents information to facilitate technology transfer and to provide a clear guide for warm water fish pond construction and management. Major topic areas considered include: (1) selecting the site and type of fish farm; (2) selecting the appropriate fish; (3) constructing, preparing, managing, and harvesting the pond; (4) preserving fish; (5) problems of fish in ponds; and (6) methods of fish culture in places where ponds are not possible. A list of resources on various aspects of fish pond operation is included in an appendix. (CW)
Hubbard ladders in a magnetic field
The behavior of a two leg Hubbard ladder in the presence of a magnetic field is studied by means of Abelian bosonization. We predict the appearance of a new (doping dependent) plateau in the magnetization curve of a doped 2-leg spin ladder in a wide range of couplings. We also discuss the extension to N-leg Hubbard ladders.
National guidance for assessing the risks of consuming chemically contaminated fish
The EPA`s Fish Contamination Program (FCP) provides technical assistance and guidance to State, Federal, and Tribal agencies for assessing human health risks associated with dietary exposure to chemically contaminated noncommercial freshwater and estuarine fish and shellfish. the program includes guidance documents which may be used for developing fish consumption advisories. One series of guidance documents, titled Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, are designed to provide the States, Tribes, and other interested parties with a scientifically defensible, cost effective methodology for developing, implementing, managing and communicating risk-base fish consumption advisories. The fist document of the series, Volume 1: Fish Sampling and Analysis (September 1993), provides recommended methods for sampling strategies, field collection procedure, chemical analysis, data management and profiles of 24 chemicals which have been identified as analyses of concern with respect to dietary exposure to chemical contaminants in fish. volume 2: Risk Assessment and Fish Consumption Limits (June 1994), provides chemical specific risk based fish consumption limits for 24 analyses based on the amount and frequency of individual fish consumption patterns. Specific fish consumption limits and advice for the general population and women of child-bearing age are provided. Volume 3: Risk Management, is scheduled for release in late 1 994. This document will review management options which should be considered in the development of fish consumption advisories. Volume 4: Risk Communication, is scheduled for release in the fall of 1994. This document will address effective communication of fish consumption advisories to targeted audiences.
Behavioural systems to guide or divert fish at hydro-electric facilities
A study was conducted to determine the effectiveness of selected behavioural systems in guiding or diverting various riverine fish species at hydro-electric facilities, and to assess the potential of using such systems to divert downstream migrants away from the turbines towards a fish bypass, or to direct upstream migrants towards a fish handling facility such as a fish ladder. Strobe lights and a pneumatic popper (sound) were evaluated as deterrents whereas a filtered mercury vapour light source was tested as a possible attractant. In laboratory tests, coho salmon, Atlantic salmon, rainbow trout and lake whitefish showed an avoidance of strobe light which varied with current and time of day. However, at a current velocity approaching 1 m/s strobe light was not effective as a repellent. Chinook salmon showed only a slight avoidance of this light source which may be partly related to their size. Preliminary gross response tests with a pneumatic popper indicated that most species tested displayed a slight avoidance response. In field tests, rainbow trout and coho salmon showed a marked avoidance of strobe light, which was consistent with the laboratory results. Field test results with a pneumatic popper indicated that coho salmon did not avoid this deterrent. Filtered mercury vapour light was not effective in attracting selected salmonid species in either laboratory or field tests. Results from this study were compared with other research conducted on non-salmonid species. Several inconsistencies were found in the responses of different fish species to various deterrents. For each potential application of a behavioural system, there is the need for site-specific fine tuning of that system in response to local and seasonal variation of the migrating species. 26 refs., 21 figs., 5 tabs.
This report summarizes activities conducted by the Oregon Department of Fish and Wildlife's Juvenile Outmigration and Survival M&E project in the Umatilla River subbasin between 2004-2006. Information is used to make informed decisions on hatchery effectiveness, natural production success, passage improvement and flow enhancement strategies. Data collected includes annual estimates of smolt abundance, migration timing, and survival, life history characteristics and productivity status and trends for spring and fall Chinook salmon, coho salmon and summer steelhead. Productivity data provided is the key subbasin scale measure of the effectiveness of salmon and steelhead restoration actions in the Umatilla River. Information is also used for regional planning and recovery efforts of Mid-Columbia River (MCR) ESA-listed summer steelhead. Monitoring is conducted via smolt trapping and PIT-tag interrogation at Three Mile Falls Dam. The Umatilla Juvenile Outmigration and Survival Project was established in 1994 to evaluate the success of management actions and fisheries restoration efforts in the Umatilla River Basin. Project objectives for the 2004-2006 period were to: (1) operate the PIT tag detection system at Three Mile Falls Dam (TMFD), (2) enhance provisional PIT-tag interrogation equipment at the east bank adult fish ladder, (3) monitor the migration timing, abundance and survival of naturally-produced juvenile salmonids and trends in natural production, (4) determine migration parameters and survival of hatchery-produced fish representing various rearing, acclimation and release strategies, (5) evaluate the relative survival between transported and non-transported fish, (6) monitor juvenile life history characteristics and evaluate trends over time, (7) investigate the effects of river, canal, fishway operations and environmental conditions on smolt migration and survival, (8) document the temporal distribution and diversity of resident fish species, and (9) participate in planning and coordination activities within the basin and dissemination of results.
Abstract in portuguese O presente estudo avalia a sazonalidade da ascensão de peixes na escada experimental localizada junto à barragem da hidrelétrica de Itaipu e as relações com a reprodução, temperatura, nível do rio e suas vazões. As amostragens de peixes e de fatores abióticos foram obtidas de fevereiro de 1995 a janeiro de 1997, em dois tanques de descanso, aqui denominados Caixas, sendo o primeiro, Caixa A, localizado na altura de 10 m da cota média do rio Paraná, e o segundo (more) , Caixa B, ao final da escada, a 27 m de altura. Modelos autoregressivos foram empregados com o objetivo de remover a dependência temporal entre as observações. Posteriormente, os resíduos dos modelos foram utilizados para relacionar, através do coeficiente de correlação de Pearson, as densidades de peixes com as variáveis ambientais. Padrões claros de sazonalidade foram observados para a variável temperatura. As variáveis cota, vazão turbinada e vazão vertida, embora não tenham apresentado dinâmicas sazonais claras, foram temporalmente dependentes, tendo em vista que os coeficientes de autocorrelação, para diferentes k, foram significativos. Na amostragem de peixes de maior porte foram registradas 23 espécies ao longo dos dois anos de estudo, sendo que a maior parte dos indivíduos não se encontrava em reprodução e cerca de 90% dos indivíduos amostrados pertenciam a apenas seis espécies. A variabilidade temporal da abundância total, considerando o conjunto das espécies, indicou um padrão de variação sazonal, permitindo evidenciar que os maiores valores de abundância foram observados sistematicamente nos meses de outubro a fevereiro (75%) e na caixa A (61% dos 1343 indivíduos amostrados). Os menores valores foram registrados nos meses de inverno. O correlograma da densidade total confirma essa tendência. Nas amostragens destinadas às espécies de pequeno porte, foram registradas seis espécies, todas presentes em ambas as caixas, sendo mais freqüentes na primeira (64%). Entre essas espécies, Bryconamericus stramineus contribuiu com 99% do total amostrado. A variabilidade temporal da abundância total das espécies de pequeno porte indicou um padrão distinto de variação sazonal entre dois períodos anuais analisados. Abstract in english We evaluated the seasonality of the ascent of fish in an experimental ladder located close to Itaipu Dam, and its relationships to reproduction, temperature, river level and discharge. Samplings of fish and measurements of abiotic factors were performed from February, 1995 through January, 1997, in two resting pools of the ladder. Pool A was located 10 m above the mean level of the Paraná River; Pool B was located at the upper end of the ladder, at a height of 27 m. Auto (more) regressive models were used to remove the temporal dependence between observations. Residuals of the models were used to relate, using Pearson's correlation coefficient, the densities of fish to the environmental variables. Clear patterns of seasonality were observed for temperature. River level, turbine discharge and spillway discharge, although they showed no clear seasonal dynamics, were time-dependent, because the autocorrelation coefficients for different k values were significant. In the samples of larger-sized fish, 23 species were recorded during the two years of study. Most of the individuals were in a non reproductive state, and about 90% of the individuals belonged to only six species. The temporal variability in total abundance, considering all species, indicated a pattern of seasonal variation; high abundances occurred from October through February (75%) and in Pool A (61% of the 1343 individuals sampled). Low abundances were recorded during winter, with the correlogram of total density confirming this tendency. In the samples of smaller-sized species, six species were recorded, with all six present in both pools, but more frequent in the first pool (64%). Bryconamericus stramineus comprised 99% of the total numbers of smaller fish in the samples. The total abundance of small species showed different temporal patterns of seasonal variation in the two years of study.
The Dalles Dam, Columbia River: Spillway Improvement CFD Study
This report documents development of computational fluid dynamics (CFD) models that were applied to The Dalles spillway for the US Army Corps of Engineers, Portland District. The models have been successfully validated against physical models and prototype data, and are suitable to support biological research and operations management. The CFD models have been proven to provide reliable information in the turbulent high-velocity flow field downstream of the spillway face that is typically difficult to monitor in the prototype. In addition, CFD data provides hydraulic information throughout the solution domain that can be easily extracted from archived simulations for later use if necessary. This project is part of an ongoing program at the Portland District to improve spillway survival conditions for juvenile salmon at The Dalles. Biological data collected at The Dalles spillway have shown that for the original spillway configuration juvenile salmon passage survival is lower than desired. Therefore, the Portland District is seeking to identify operational and/or structural changes that might be implemented to improve fish passage survival. Pacific Northwest National Laboratory (PNNL) went through a sequence of steps to develop a CFD model of The Dalles spillway and tailrace. The first step was to identify a preferred CFD modeling package. In the case of The Dalles spillway, Flow-3D was as selected because of its ability to simulate the turbulent free-surface flows that occur downstream of each spilling bay. The second step in development of The Dalles CFD model was to assemble bathymetric datasets and structural drawings sufficient to describe the dam (powerhouse, non-overflow dam, spillway, fish ladder entrances, etc.) and tailrace. These datasets are documented in this report as are various 3-D graphical representations of The Dalles spillway and tailrace. The performance of the CFD model was then validated for several cases as the third step. The validated model was then applied to address specific SIS design questions. Specifically, the CFD models were used to evaluate flow deflectors, baffle block removal and the effects of spillwalls. The CFD models were also used to evaluate downstream differences at other locations, such as at the Highway 197 bridge piers and Oregon shore islands, due to alterations in spill pattern. CFD model results were analyzed to quantitatively compare impacts of the spillwall that has subsequently been constructed between bays 6 and 7. CFD model results provided detailed information about how the spillwall would impact downstream flow patterns that complemented results from the 1:80 scale physical model. The CFD model was also used to examine relative differences between the juvenile spill pattern used in previous years and the anticipated spill pattern that will be applied once the wall is complete. In addition, the CFD model examined velocity magnitudes over the downstream basalt shelf to investigate potential for erosion under high flow conditions (e.g., 21 kcfs/bay for bays 1 through 6) with the spillwall in place. Several appendices follow the results and discussion sections of this report. These appendices document the large number of CFD simulations that have been performed by PNNL; both spillway improvement study (SIS) related and those performed for related biological tests.
Sea-cage aquaculture, sea lice, and declines of wild fish.
A sea cage, sometimes referred to as a net pen, is an enclosure designed to prevent farm fish from escaping and to protect them from large predators, while allowing a free flow of water through the cage to carry away waste. Farm fish thus share water with wild fish, which enables transmission of parasites, such as sea lice, from wild to farm and farm to wild fishes. Sea lice epidemics, together with recently documented population-level declines of wild salmon in areas of sea-cage farming, are a reminder that sea-cage aquaculture is fundamentally different from terrestrial animal culture. The difference is that sea cages protect farm fish from the usual pathogen-control mechanisms of nature, such as predators, but not from the pathogens themselves. A sea cage thus becomes an unintended pathogen factory. Basic physical theory explains why sea-cage aquaculture causes sea lice on sympatric wild fish to increase and why increased lice burdens cause wild fish to decline, with extirpation as a real possibility. Theory is important to this issue because slow declines of wild fish can be difficult to detect amid large fluctuations from other causes. The important theoretical concepts are equilibrium, host-density effect, reservoir-host effect, and critical stocking level of farmed fish (stocking level at which lice proliferate on farm fish even if wild fish are not present to infect them). I explored these concepts and their implications without mathematics through examples from salmon farming. I also considered whether the lice-control techniques used by sea-cage farmers (medication and shortened grow-out times) are capable of protecting wild fish. Elementary probability showed that W ? W* - ?F (where W is the abundance of wild fish, W* is the prefarm abundance, F is the abundance of farm fish, and ? is the ratio of lice per farm fish to lice per wild fish). Declines of wild fish can be reduced by short growing cycles for farm fish, medicating farm fish, and keeping farm stocking levels low. Declines can be avoided only by ensuring that wild fish do not share water with farmed fish, either by locating sea cages very far from wild fish or through the use of closed-containment aquaculture systems. These principles are likely to govern any aquaculture system where cage-protected farm hosts and sympatric wild hosts have a common parasite with a direct life cycle. PMID:19128323
While the cascading effect of piscivorous fish on the pelagic food-web has been well studied in north temperate lakes, little is known about the role of native piscivores in warm lakes. Here, the fish communities are typically characterized by high abundances of small, omnivorous fish exerting a high predation pressure on the zooplankton. We conducted a 1-month replicated mesocosm experiment at subtropical conditions to test the effects of piscivorous (Hoplias malabaricus) fish on phytoplankton biomass and water transparency. Our experimental design comprised two (phytoplankton?+?zooplankton), three (phytoplankton?+?zooplankton?+?planktivores) and four (phytoplankton?+?zooplankton?+?planktivores?+?piscivores) trophic levels. We designed two different four trophic level treatments, one with...
Alignment of the MSGC barrel support structure
The MSGC barrel is a sub-part of the tracking system of the CMS experiment at the LHC. The mechanical support structure of the MSGC barrel consists of ladder-like support beams carrying the detector modules and of four disks supporting the ladders. The required alignment precision of the modules, a few tens of micrometers, is designed to be obtained by precise part manufacture and by careful measurement of the alignment during the assembly of the structure. In the paper the use of digital photogrammetry for the measurement of the alignment of the disks and for the structural verification is presented. Digital photogrammetry was chosen from a number of potential methods after a careful evaluation. The use of photogrammetry for the structural verification of a prototype is presented. The displacements were measured both of unloaded and loaded disk by using photogrammetry and linear displacement transducers for verification. The displacements obtained from the two measurement methods corresponded well, not only to each other, but also to the results given by finite element analysis. The structural verification will be done and the alignment procedure will be tested with a full-sized prototype of a half of the MSGC barrel. Preparations for the photogrammetry measurements are presented and the design of the required supplementary equipment is shown. (authors)
Means-end chains as associative networks: Do they exhibit automatic spreading activation
Despite its popularity in consumer research, means-end chain theory suffers from problems of unconfirmed validity. In particular, it is unknown if its central construct, the means-end chain (MEC), is an identifiable memory structure or not. Theoretically, means-end chains can be cast as associative networks with a three-layered structure, consisting of attributes (A), consequences (C) and values (V) that are hierarchically linked. This yields two predictions when operationalized in a lexical decision task: means-end chains should display spreading activation (direct as well as mediated priming) and automatic activation (persistence of priming effects under short inter-stimulus intervals and high proportions of non-words). The predictions were tested in two experiments. Both experiments used a single-presentation lexical decision task with eleven classes of stimuli: AC, CV and AV pairs collected from the respective participant by means of a laddering interview in a pilot session (on average, 85 days prior to the experiment); AC, CV and AV pairs from a laddering interview with a different participant (checked for non-overlap); word pairs with direct, mediated and no associations from a standardized word list; and finally, non-words and fillers serving as distractors. In Experiment 1, long inter-stimulus intervals (750 msec) and a low proportion of non-words (25%) were used. 44 undergraduates participated. Significant priming effects were observed for AC and CV pairs, but not for AV pairs. Surprisingly, the priming effects did not differ depending on whether the word pairs had been taken from the participant's own laddering interview, or a different participant's interview. In Experiment 2, short inter-stimulus intervals (250 msec) and a high proportion of non-words (40%) were used. 46 undergraduates participated. Significant priming effects were observed for AC, CV and AV pairs. Priming effects for CV and AV pairs were larger than for AC pairs. Again, the size of the priming effects did not differ depending on whether the word pairs had been taken from the participant's own laddering interview, or a different participant's interview. Largely, the pattern of results was in line with the predictions. In Experiment 2, direct priming (AC and CV pairs) as well as mediated priming (AV pairs) could be observed, consistent with the spreading-activation assumption. Furthermore, results in Experiment 2 were obtained under conditions designed to suppress strategic processing, suggesting that the automaticity assumption may hold as well. However, some qualifications have to be made. The absence of mediated priming effects (AV pairs) in Experiment 1 but not in Experiment 2 suggests that post-lexical, strategic processes may have overridden a priming effect. This is a phenomenon not uncommon in the literature, especially when the number of unassociated words in a word list is so low that participants are led to expect a link between adjacent words. More surprising is the uniformity of priming effects with regard to the source of thestimuli, i.e. whether they were taken from a participant's own laddering interview or other participants' laddering interviews. At this point, the only explanation appears to be that the associations were relatively uniform across subjects.
In this work, a novel metaheuristic named artificial fish school algorithm is introduced into the optimization of pump parameters for the design of gain flattened Raman fiber amplifiers for the first time. Artificial fish school algorithm emulates three simple social behaviors of a fish in a school, namely, preying, swarming and following, to optimize a target function. In this algorithm the pump wavelengths and power levels are mapped respectively to the state of a fish in a school, and the gain of a Raman fiber amplifier is mapped to the concentration of a food source for the fish school to search. Application of this algorithm to the design of a C-band gain flattened Raman fiber amplifier leads to an optimized amplifier that produces a flat gain spectrum with 0.63 dB in band ripple for given conditions. This result demonstrates that the artificial fish school algorithm is efficient for the optimization of pump parameters of gain flattened Raman fiber amplifiers.
BACKGROUND: Epidemiologic evidence of an association between fish intake and type 2 diabetes (T2D) is inconsistent and unresolved. OBJECTIVE: The objective was to examine the association between total and type of fish intake and T2D in 8 European countries. DESIGN: This was a case-cohort study, nested within the European Prospective Investigation into Cancer and Nutrition (EPIC) study, with 3.99 million person-years of follow-up, 12,403 incident diabetes cases, and a random subcohort of 16,835 individuals from 8 European countries. Habitual fish intake (lean fish, fatty fish, total fish, shellfish, and combined fish and shellfish) was assessed by country-specific dietary questionnaires. HRs were estimated in each country by using Prentice-weighted Cox regression models and pooled by using a random-effects meta-analysis. RESULTS: No overall association was found between combined fish and shellfish intake and incident T2D per quartile (adjusted HR: 1.00; 95% CI: 0.94, 1.06; P-trend = 0.99). Total fish, lean fish, and shellfish intakes separately were also not associated with T2D, but fatty fish intake was weakly inversely associated with T2D: adjusted HR per quartile 0.97 (0.94, 1.00), with an HR of 0.84 (0.70, 1.01), 0.85 (0.76, 0.95), and 0.87 (0.78, 0.97) for a comparison of the second, third, and fourth quartiles with the lowest quartile of intake, respectively (P-trend = 0.06). CONCLUSIONS: These findings suggest that lean fish, total fish, and shellfish intakes are not associated with incident diabetes but that fatty fish intake may be weakly inversely associated. Replication of these findings in other populations and investigation of the mechanisms underlying these associations are warranted. Meanwhile, current public health recommendations on fish intake should remain unchanged.
D0 Silicon Upgrad: D0 Silicon Cooling System
The cooling system design is not complete. This paper lays out the general design and some of the design calculations that have been performed up to this date. Further refinement will be performed. This is especially true in the piping layout, piping insulation and detector manifold areas. The silicon detector is cooled by means of a coolant in the beryllium channels that also act as the primary supporting device for the silicon ladders and wedges. The coolant is water with ethylene glycol added as a freezing point depressant. The glycol concentration in the coolant is 30% by weight resulting in a freezing point of approximately -15 C. If the water/glycol is not sufficient for maintaining the desired detector temperature the concentration of the water/glycol may be changed or an alternative coolant may be used.
Generic design of Chinese remaindering schemes
We propose a generic design for Chinese remainder algorithms. A Chinese remainder computation consists in reconstructing an integer value from its residues modulo non coprime integers. We also propose an efficient linear data structure, a radix ladder, for the intermediate storage and computations. Our design is structured into three main modules: a black box residue computation in charge of computing each residue; a Chinese remaindering controller in charge of launching the computation and of the termination decision; an integer builder in charge of the reconstruction computation. We then show that this design enables many different forms of Chinese remaindering (e.g. deterministic, early terminated, distributed, etc.), easy comparisons between these forms and e.g. user-transparent parallelism at different parallel grains.
John Day Fish Passage and Screening; 2001 Annual Report.
The accomplishments of the John Day, Umatilla, and Walla Walla Fish Passage and Screening Programs include the following: Operation and maintenance of 364 existing fish screening devices (see Table 4), replacement of 18 outdated fish screening devices that totaled 31 rotary drums (some were multiple drum systems), 4 new screens at unscreened diversions, 26 pump intake fish screens, fabrication of components for 16 additional fish screens for the Rogue basin, construction of two fish passage structures, and participation in other activities. After the replacement or construction of 22 fish screening devices during 2001, we now have 108 screening devices that meet NMFS criteria. Funding for these projects was attained from BPA, NMFS and OWEB. The John Day Fish Passage and Screening Program focused construction efforts into new and replacement fish screening devices for these various programs throughout the state of Oregon. The program also continued to develop and implement innovative designs to meet the diverse and expanding needs for the state of Oregon. Projects completed during this report period meet the current National Marine Fisheries Service (NMFS) criteria. Fish species targeted for protection include ESA Listed Mid-Columbia steelhead, Columbia basin bull trout, anadromous and resident salmonids, and numerous non-game fish species. Priority project locations have been identified as the upper reaches of the Middle Fork, North Fork, South Fork and the Mainstem of the John Day River and their tributaries. These upper reaches contain critical salmon and steelhead spawning and rearing habitat.
Electrofishing survey of the Great Miami River. Annual report, September 1993
Fish sampling by electroshocking in the Great Miami River above and below the Fernald sit was designed to determine changes in the health of the fish community compared to the previous nine years and to collect samples for uranium analysis in fish filets. This document contains information describing the findings of this program. Topics discussed include: physical and chemical parameters, species richness, species diversity, and water analysis.
We developed and tested an efficient, portable, cost effective, pass-through system to detect downstream migrating salmonids marked with 12mm passive integrated transponder (PIT) tags. The design combines two established technologies, one for the capture of downstream migrating salmonid (the rotary screw trap), and one for fish detection (12mm PIT tag technology). Detection rates were 100% for PIT-tagged fish passed through separately, and 95% for PIT-tagged fish passed through in pairs.
The possible requirement of facilities to move migrating fish upstream around dams may be a factor in determining the feasibility of retrofitting small dams for hydroelectric generation. Basic design considerations are reported that should be evaluated on a site-specific basis if upstream fish passage facilities are being considered for a small scale hydroelectric project (defined as an existing dam that can be retrofitted to generate 25 MW or less). Information on general life history and geographic distribution of fish species that may require passage is presented. Biological factors important in the design of upstream passage facilities are discussed: gas bubble disease, fish swimming speed, oxygen consumption by fish, and diel and photo behavior. Three general types of facilities (fishways, fish locks, and fish lifts) appropriate for upstream fish passage at small scale hydroelectric projects are described, and size dimensions are presented. General design criteria for these facilities (including fish swimming ability and behavior) and general location of facilities at a site are discussed. Basic cost considerations for each type of passage facility, including unit cost, operation and maintenance costs, and costs for supplying attraction water, are indicated.
Research to improve extension ladder angular positioning.
A leading cause for extension ladder fall incidents is a slide-out event usually related to suboptimal ladder inclination. An improved ladder positioning method or procedure could reduce the risk of ladder stability failure and the related fall injury. The objective of the study was to comparatively evaluate the effectiveness of a multimodal angle indicator with other existing methods for extension ladder angular positioning. Twenty experienced and 20 inexperienced ladder users participated in the study. Four ladder positioning methods were tested in a controlled laboratory environment with 4.88 m (16 ft) and 7.32 m (24 ft) ladders in extended and retracted positions. The positioning methods included a no-instruction method, the current standard anthropometric method, and two instrumental methods - a bubble level indicator, and a multimodal indicator providing direct feedback with visual and sound signals. Performance measures included positioning angle and time. The results indicated that the anthropometric method was effective in improving the extension ladder positioning angle (p ANSI A14 standard anthropometric method in ladder angular positioning. The multimodal indicator concept has been further developed to become a hand-held tool in the form of a smart phone application. PMID:23177178
A summary of 22 Years of Fish Screen Evaluation in the Yakima River Basin, Summary Report 1985-2007.
Sixty fish screen facilities were constructed in the Yakima River basin between 1985 and 2006 as part of the Northwest Power and Conservation Council plan to mitigate the effects of federal hydroelectric projects on fish and wildlife populations. This report summarizes evaluations of some of those and other fish screen facilities conducted by Pacific Northwest National Laboratory (PNNL) from 1985 through 2006. The objective of these studies was to determine if the newly designed and constructed fish screens were effective at providing juvenile salmonids safe passage past irrigation diversions. To answer that question, PNNL conducted release-and-catch studies at eight Phase I sites in the Yakima River basin. Increasing concerns about the impacts of hatchery fish releases on the wild fish population, as well as the cost and time necessary to perform these kinds of biological studies at more than 60 planned Phase II sites, required development of techniques to evaluate the effectiveness of the sites without releasing fish. The new techniques involved collecting information on screen design, operation, and effectiveness at guiding fish safely through the fish screen facility. Performance measures including water velocities and passage conditions provide a good alternative to biological studies at significantly lower cost and time. Physical techniques were used at all 10 Phase I and 28 Phase II sites evaluated by PNNL over the following 19 years. Results of these studies indicate the Phase I and II fish screen facilities are designed and capable of providing safe passage for juvenile salmonids so long as construction, maintenance, and operations meet the criteria used in the design of each site and the National Marine Fisheries Service criteria for juvenile fish screen design.
Current status of the OPAL silicon microvertex project
For the 1991 run of the OPAL detector at LEP, a complete two layer single-sided silicon microstrip detector array was installed around the beam-pipe. The detectors employed a novel biassing technique for the capacitively read out diode strips (Field Effect Transistors integrated on the silicon wafer) and the MX5 microplex read-out chip was used for the first time. The performance measured in OPAL for the ladders of 3 daisy-chained detectors (length 180mm) is very good with signal/noise of 22:1. The resolution of the detectors (measured to be around 5[mu]m from test beam investigations) has allowed improvement in the impact parameter resolution of OPAL by greater than a factor of two. The beauty, tau and charm physics potential that this opens up is now being exploited. OPAL has plans to further upgrade to a detector giving both co-ordinates of the track intersection with the silicon barrels. To achieve this, ladder assemblies using two detectors of orthogonal strips glued back-to-back are being used. In addition, double-sided detectors of novel design have been fabricated and are being evaluated. Results from OPAL data taking at LEP, test beam results and laboratory measurements are presented. Both the performance of the detectors currently used in OPAL and those intended for future upgrades are discussed. (orig.)
Puffer fish, Takifugu niphobles, collected from the Hong Kong coastal waters were screened for tetrodotoxin-producing bacteria. A Gram-negative, non-acid-fast, non-sporing and rod shaped bacterial strain (designated as gutB01) was isolated from the intestine of the puffer fish and was shown to produ...
High-performance liquid chromatography was used to quantify residues of oxolinic acid and flumequine in muscle of wild fish caught in the vicinity of fish farms using the LiftUp feed collector system or a hydroacoustic feed detector during medication. Both systems are designed to minimise feed waste...
Objective: To measure the bioavailability of selenium from cooked and raw fish in humans by estimating and comparing apparent absorption and retention of selenium in biosynthetically labelled fish with labelled selenate and biosynthetically labelled selenium in brewers yeast. Design: The interventio...
The present study was designed to compare the responses in freshwater fish Oreochromis niloticus exposed to a synthetic pyrethroid, cypermethrin (CYP); an essential metal, copper (Cu); and a nonessential metal, lead (Pb). Fish were exposed to 0.05 ?g/l CYP, 0.05 mg/l Cu, and 0.05 mg/l Pb for 4 and 2...
Threemile Falls Dam (Threemile Dam), located near the town of Umatilla, is the major collection and counting point for adult salmonids returning to the Umatilla River. Returning salmon and steelhead were enumerated at Threemile Dam from June 7, 2007 to August 11, 2008. A total of 3,133 summer steelhead (Oncorhynchus mykiss); 1,487 adult, 1,067 jack, and 999 subjack fall Chinook (O. tshawytscha); 5,140 adult and 150 jack coho (O. kisutch); and 2,009 adult, 517 jack, and 128 subjack spring Chinook (O. tshawytscha) were counted. All fish were enumerated at the east bank facility. Of the fish counted, 1,442 summer steelhead and 88 adult and 84 jack spring Chinook were hauled upstream from Threemile Dam. There were 1,497 summer steelhead; 609 adult, 1,018 jack and 979 subjack fall Chinook; 5,036 adult and 144 jack coho; and 1,117 adult, 386 jack and 125 subjack spring Chinook either released at, or allowed to volitionally migrate past, Threemile Dam. Also, 110 summer steelhead; 878 adult and 43 jack fall Chinook; and 560 adult and 28 jack spring Chinook were collected as broodstock for the Umatilla River hatchery program. In addition, there were 241 adult and 15 jack spring Chinook collected at Threemile Dam for outplanting in the South Fork Walla Walla River and Mill Cr, a tributary of the mainstem Walla Walla River. The Westland Canal juvenile facility (Westland), located near the town of Echo at river mile (RM) 27, is the major collection point for out-migrating juvenile salmonids and steelhead kelts. The canal was open for 158 days between February 11, 2008 and July 18, 2008. During that period, fish were bypassed back to the river 150 days and were trapped 6 days. There were also 2 days when fish were directed into and held in the canal forebay between the time the bypass was closed and the trap opened. An estimated 64 pounds of fish were transported from the Westland trapping facility. Approximately 25.8% of the fish transported were salmonids. In addition, one adult Pacific lamprey was trapped and released above the Westland ladder this year. The Threemile Dam west bank juvenile bypass was opened on March 11, 2008 in conjunction with water deliveries and continued through the summer. West Extension Irrigation District (WEID) discontinued diverting live flow on June 24, 2008 but the bypass remained open throughout the project year. The juvenile trap was not operated this project year.
Comparison of Outcomes of Permanently Closed and Periodically Harvested Coral Reef Reserves
Abstract: In many areas of the developing world, the establishment of permanent marine reserves is inhibited by cultural norms or socioeconomic pressures. Community conserved areas that are periodically harvested are increasingly being implemented as fisheries management tools, but few researchers have empirically compared them with permanently closed reserves. We used a hierarchal control-impact experimental design to compare the abundance and biomass of reef fishes, invertebrates, and substrate composition in periodically harvested and permanent reserves and in openly fished (control sites) of the South Pacific island country of Vanuatu. Fished species had significantly higher biomass in periodically harvested reserves than in adjacent openly fished areas. We did not detect differences i...
The lunar cycle determines availability of coral-reef fishes at fish markets
During 139 visits between March 2009 and May 2011, it was found that the availability of reef fishes at a local fish market in the Philippines was highly affected by the lunar cycle. The number of vendors selling reef fishes was significantly lower (134%) during third lunar quarters (full moon periods) than during the first, second and fourth lunar quarters (402, 250 and 300%, respectively). It is recommended that the influence of the lunar cycle on fish availability is considered when designing sampling strategies for catch surveys.
The effectiveness of natural compounds in slowing down the microbial quality decay of refrigerated fish hamburger is addressed in this study. In particular, the control of the microbiological spoilage by combined use of three antimicrobials, and the determination of their optimal composition to extend the fish hamburger Microbiological Stability Limit (MAL) are the main objectives of this work. Thymol, grapefruit seed extract (GFSE) and lemon extract were tested for monitoring the cell growth of the main fish spoilage microorganisms (Pseudomonas fluorescens, Photobacterium phosphoreum and Shewanella putrefaciens), inoculated in fish hamburgers, and the growth of mesophilic and psychrotrophic bacteria. A Central Composite Design (CCD) was developed to highlight a possible synergic effect of...
Since 2005, restrictions have been because of fish consumption along the Rhone River because of high polychlorobiphenyl (PCB) concentrations, which have resulted inadverse economic consequences for professional fisheries in affected areas. French environmental authorities have expended considerable efforts to research sediment remediation strategies and development of sediment quality guidelines designed to protect the health of humans consuming Rhône River fish. Here we: (1) develop a bioaccumulation food-web model that describes PCB concentrations in three common freshwater fish species of the Rhône River, using Bayesian inference to estimate the input parameters; (2) test the predictive power of the model in terms of risk assessment for fish consumption; and (3) discuss ...
Denitrification is applied in the tertiary treatment of wastewater to reduce nitrogen pollution. Fluorescence in situ hybridization (FISH), catalyzed reporter deposition (CARD)-FISH, cloning, and scanning electron microscopy (SEM) were applied to follow the evolution of the microbial composition and structure of granular sludge in chemolithotrophic denitrifying bioreactors fed with nitrate and thiosulfate. FISH oligonucleotide probes for the chemolitoautotrophic denitrifiers Thiobacillus denitrificans and Thiomicrospira denitrificans were designed and their utility tested. CARD-FISH and cloning data showed that bacterial diversity in the biofilms changed during the reactor operation. Chemoorganotrophic fermentative Gram-positive strains in the phyla, Actinobacteria and Firmicutes, were dom...
A reconfigurable fully differential programmable linear-phase filter is presented in the paper which can be used in portable multichannel electroencephalogram, electrocardiogram, wireless brain-heart monitoring systems used in hospital or home care settings. To reduce the influence of coefficient sensitivity and maintain an undistorted bio-signal, a fifth-order ladder-type Bessel Transconductance-Capacitor (Gm-C) lowpass filter is employed. The filter is designed as part of on-chip self-adjusting noise suppression block and its programmability makes it suitable for different biomedical signals processing. Simulation results show that this low-voltage and low-power filter possesses the HD3 of 50.7dB, dynamic range of 62dB, dc gain of -2.899dB, and power consumption of 30µW. Meanwhile, the designed filter can effectively reduce self-adjusting noise on specific channels and anti-aliasing of the analog-to-digital converter (ADC).
The sampling theory is basic and crucial in engineering sciences. On the other hand, the linear canonical transform (LCT) is also of great power in optics, filter design, radar system analysis and pattern recognition, etc. The Fourier transform (FT), the fractional Fourier transform (FRFT), Fresnel transform (FRT) and scaling operations are considered as special cases of the LCT. In this paper, we structure certain types of non-bandlimited signals based on two ladder-shape filters designed in the LCT domain. Subsequently, these non-bandlimited signals are reconstructed from their samples together with the generalized sinc function, their parameter M-Hilbert transforms or their first derivatives and other information provided by the phase function of the nonlinear Fourier atom which is the ...
Design and test of the final ALICE SDD CARLOS end ladder board
The paper presents the design and test of the final prototype of the CARLOS (Compression And Run Length Encoding Subsystem) end ladder board that is going to be used in the ALICE experiment at CERN. This board is able to compress data coming from one Silicon Drift Detector (SDD) front-end electronics and to send them towards the data concentrator card CARLOSrx in counting room via a 800 Mb/s optical link. The board design faces several constraints, mainly size (54x49 mm) and radiation tolerance: for this reason the board contains several CERN developed ASICs. A test setup has been realized for selecting the good devices among the 500 cards already produced.
Universal protocol for generating 100bp size standard for endless usage
Abstract in english Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse pr (more) imers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.
FISH Finder: a high-throughput tool for analyzing FISH images.
MOTIVATION: Fluorescence in situ hybridization (FISH) is used to study the organization and the positioning of specific DNA sequences within the cell nucleus. Analyzing the data from FISH images is a tedious process that invokes an element of subjectivity. Automated FISH image analysis offers savings in time as well as gaining the benefit of objective data analysis. While several FISH image analysis software tools have been developed, they often use a threshold-based segmentation algorithm for nucleus segmentation. As fluorescence signal intensities can vary significantly from experiment to experiment, from cell to cell, and within a cell, threshold-based segmentation is inflexible and often insufficient for automatic image analysis, leading to additional manual segmentation and potential subjective bias. To overcome these problems, we developed a graphical software tool called FISH Finder to automatically analyze FISH images that vary significantly. By posing the nucleus segmentation as a classification problem, compound Bayesian classifier is employed so that contextual information is utilized, resulting in reliable classification and boundary extraction. This makes it possible to analyze FISH images efficiently and objectively without adjustment of input parameters. Additionally, FISH Finder was designed to analyze the distances between differentially stained FISH probes. AVAILABILITY: FISH Finder is a standalone MATLAB application and platform independent software. The program is freely available from: http://code.google.com/p/fishfinder/downloads/list. PMID:21310746
Biliary PAH metabolites and the hepatosomatic index of brown bullheads from Lake Erie tributaries
In studies designed to investigate the environmental exposure of fish in Lake Erie tributaries, a benthic fish, the brown bullhead (Ameiurus nebulosus), was collected from the industrially contaminated Detroit River, Ottawa River, Black River, Cuyahoga River-harbor and -upstream, Ashtabula River, Buffalo River, and Niagara River, and the non-industrialized Old Woman Creek during 1997-2000. Biliary benzo[a]pyrene (B[a]P)- and naphthalene (NAPH)-type metabolites and the hepatosomatic index (HSI) were measured in fish and compared between different sites. Fish from all of the contaminated sites except Niagara River had significantly higher concentrations of both types of polycyclic aromatic hydrocarbon (PAH) metabolites than fish from the Old Woman Creek. Concentrations of PAH metabolites in bile of fish were positively associated with concentrations of PAHs in sediments, supporting the use of bile metabolites as a measure of PAH exposure. Relatively low concentrations of PAHs detected in fish bile and sediments of the Niagara River, which had undergone extensive remediation, suggested a lowered PAH exposure for fish at this site. No apparent trend was observed in HSI between the industrialized and non-industrialized sites. This study demonstrates that biliary PAH metabolites are an effective indicator of exposure of fish to PAHs. However, because factors other than contamination could also affect the liver size of wild fish, HSI alone may be not a reliable biomarker for assessing contaminant stress. ?? 2005 Elsevier Ltd. All rights reserved.
Variation of mercury in fish from Massachusetts lakes based on ecoregion and lake trophic status
Twenty-four of the state`s least-impacted waterbodies were sampled for sediment, water, physical characteristics and 3 species of fish to determine the extent of, and patterns of variation in, mercury contamination. Sampling effort was apportioned among three different ecological subregions of the state, as defined by EPA, and among lakes of differing trophic status. The authors sought to partition the variance to discover if these broadly defined concepts are suitable predictors of mercury levels in fish. Mean fish mercury was 0.14 ppm wet weight in samples of 168 of the bottom feeding brown bullheads (Ameriurus nebulosus) (range = 0.01--0.79 ppm); 0.3 ppm in 199 of the omnivorous yellow perch (Perca flavescens) (range = 0.01--0.75 ppm); and 0.4 ppm in samples of 152 of the predaceous largemouth bass (Micropterus salmoides) (range = 0.05--1.1 ppm). Multivariate statistics are employed to determine how mercury concentrations in fish correlate with sediment chemistry, water chemistry, fish trophic status, fish size and age, lake and watershed size, the presence and extent of wetlands in the watershed, and physical characteristics of the lake. The survey design complements ongoing efforts begun in 1983 to test fish in a variety of waters, from which emanated fish advisories for impacted rivers and lakes. The study defines a baseline for fish contamination in Massachusetts lakes and ponds that serves as a template for public health decisions regarding fish consumption.
A field study was conducted at Lower Granite Dam on the Snake River in 1992 to evaluate the feasibility of using time-lapse video technology to document and estimate fish ladder passage of chinook salmon Oncorhynchus tshawytscha, sockeye salmon 0. nerka, and steelhead 0. mykiss using time-lapse video technology. High quality video images were produced with a time-lapse video system operating in 72 h mode from 1 May through 31 December, 1992 and fish were counted from 1 June through 15 December. From the video record we counted 15 sockeye salmon, 3,283 summer chinook salmon, 1,022 fall chinook salmon, and 125,599 steelhead. The composite count of target species generated from the video record was similar (p = 0.617) to the estimate made by on-site counters during identical time periods indicating that the two methods were precise. Comparisons of 24 h video counts and on-site (10 and 16 h) counts showed that a significant (p < 0.001) proportion of target salmonids migrated during the nighttime when on-site counts are not typically made at Lower Granite Dam. The mean sockeye salmon fork length measured from video images was 453 mm. Mean fork-lengths reported for Snake River sockeye salmon between 1953 and 1965 were much greater ({female} = 546 mm {male} = 577 mm). Cost comparisons showed that video costs were less than half those of on-site counting methods. The video method also included the collection of additional data. A computer software demonstration program was developed that graphically illustrated the possibilities of a completely automated, computerized fish counting and identification system.
Abstract The present work was designed to evaluate the effects of various feeding regimens on rabbitfish growth and condition. Twelve juvenile rabbitfish Siganus rivulatus (7.3--0.1-g) were stocked into each of 15 tanks. Tanks were randomly assigned one of five treatments with three replicate tanks per treatment. Treatments were G-I: fish fed once daily at 08.00; G-II: fish fed once daily at 17.00; G-III: fish fed twice daily at 08.00, 17.00; G-IV: fish fed three times daily, at 08.00, 13.00, 17.00; G-V: fish fed three times daily at 08.00, 13.00, 17.00 for six consecutive days, followed by 1-day of fasting. Fish were offered a commercial diet (Golden Extruded, 450-g-kg-1 protein, 200-g-kg-1 lipids) to apparent satiation. At the conclusion of the study, weight gain of rabbitfish fed three ...
Randomized trial of weight-loss-diets for young adults varying in fish and fish oil content
Objective:To investigate the effect of including seafood and fish oils, as part of an energy-restricted diet, on weight loss in young overweight adults.Design:Randomized controlled trial of energy-restricted diet varying in fish and fish oil content was followed for 8 weeks. Subjects were randomized to one of four groups: (1) control (sunflower oil capsules, no seafood); (2) lean fish (3 x 150?g portions of cod/week); (3) fatty fish (3 x 150?g portions of salmon/week); (4) fish oil (DHA/EPA capsules, no seafood). The macronutrient composition of the diets was similar between the groups and the capsule groups, were single-blinded.Subjects:A total of 324 men and women aged 20–40 years, BMI 27.5–32.5?kg/m2 from Iceland, Spain and Ireland.Measurements:Anthropometr...
Adventures in Ichthyology: Pacific Northwest Fishes of the Lewis and Clark Expedition
Captains Meriwether Lewis and William Clark and other members of their expedition collected and identified nearly 400 species of plants and animals during the Voyage of Discovery. Of this total, 31 species of fish were included in Burrough’s summary of the natural history of the Expedition, including 12 fishes considered unknown to science at that time. While there is little doubt of the identity of fish for which Lewis and Clark provided detailed descriptions in their daily logs, other species designations were largely conjecture based on later scholars interpretation of the Lewis and Clarks account. Unlike other biological specimens encountered during the Expedition, no fishes were brought back for study. As a result, the identity of some fishes was never resolved. Many other fishes were reclassified during the past century based on updated scientific methods.
Facile Synthesis of Linear and Hyperbranched Ladder Poly(p-Phenylene)s without Structural Defects.
Linear and hyperbranched ladder polymers are facilely synthesized by a Pd(0) -catalyzed Suzuki polycondensation and Friedel-Crafts alkylation. The polymers show blue light emission with typical features of ladder polymers, such as well-resolved absorption and emission spectra, and small Stokes shifts. The polymers contain fewer structural defects and they exhibit good optical and thermal stability. No spectral change is observed after the films of ladder polymers are heated at 110 °C in air for 24 h. PMID:22836954
Electrical burn injuries of workers using portable aluminium ladders near overhead power lines.
The use of aluminium ladders around high voltage power lines has resulted in a significant number of electrical injuries and electrocutions. Workers often misjudge wire distances or lose control of fully extended ladders, thereby exposing themselves to electrocution hazard. High-voltage electrical burns of two workers using an aluminium ladder that contacted a high voltage power line are reported. The circumstances surrounding the injury, the clinical management of the case, and the methods of prevention are presented and discussed. PMID:8105800
New Model and Numerical Test of a High Temperature Pairing Mechanism in Stripes
We introduce a new model and mechanism of high temperature pairing in stripes. We propose a way to unambiguously test it by numerical simulations. For example, the implementation of our mechanism in a 6-leg t-J ladder model has the effect of making the spin gap of the doped 6-leg ladder be about 4 times bigger than the spin gap of the same ladder at half filling.
We introduce an analytical iterative method, the density matrix recursion method, to generate arbitrary reduced density matrices, and bipartite as well as multipartite entanglement of multi-legged periodic spin-1/2 quantum Heisenberg ladders with an arbitrary number of legs. We apply this technique to distinguish between even- and odd-legged ladders. Specifically, we show that while multipartite entanglement decreases with increasing system-size for even-legged ladders, it does the opposite for odd-legged ones.
Effective actions for spin ladders
We derive a path-integral expression for the effective action in the continuum limit of an AFM Heisenberg spin ladder with an arbitrary number of legs. The map is onto an O(3) nonlinear-sigma-model with the addition of a topological term that is effective only for odd-legged ladders and half-odd integer spins. We derive the parameters of the effective nonlinear-sigma-model and the behaviour of the spin gap for the case of even-legged ladders.
Pelagic longline fishing trials to shape a mitigation device of the depredation by toothed whales
Depredation is defined as the damage or removal of fish from fishing gear by predators, and is a crucial issue leading to negative impacts on both animals involved in depredation and fisheries. Depredation in longline pelagic fisheries targeting swordfish (Xiphias gladius) and tuna (Thunnus spp.) involves short-finned pilot whales (Globicephala macrorhynchus), false killer whales (Pseudorca crassidens) and some pelagic sharks. Since no long-term solution could be found to date, we investigated fishing gear improvement by deploying a technology designed to physically protect the hooked fish by hiding it to predators: the DMD (depredation mitigation device). Two types of DMDs were designed: ''spiders'' and ''socks''. The efficiency of ''spiders'' was tested in November 2007 during a fishing ...
In this work, a novel metaheuristic named artificial fish school algorithm is introduced into the optimization of pump parameters for the design of gain flattened Raman fiber amplifiers for the first time. Artificial fish school algorithm emulates three simple social behaviors of a fish in a school, namely, preying, swarming and following, to optimize a target function. In this algorithm the pump wavelengths and power levels are mapped respectively to the state of a fish in a school, and the gain of a Raman fiber amplifier is mapped to the concentration of a food source for the fish school to search. Application of this algorithm to the design of a C-band gain flattened Raman fiber amplifier leads to an optimized amplifier that produces a flat gain spectrum with 0.63dB in band ripple for g...
Smolt Responses to Hydrodynamic Conditions in Forebay Flow Nets of Surface Flow Outlets, 2007
This study provides information on juvenile salmonid behaviors at McNary and The Dalles dams that can be used by the USACE, fisheries resource managers, and others to support decisions on long-term measures to enhance fish passage. We researched smolt movements and ambient hydrodynamic conditions using a new approach combining simultaneous acoustic Doppler current profiler (ADCP) and acoustic imaging device (AID) measurements at surface flow outlets (SFO) at McNary and The Dalles dams on the Columbia River during spring and summer 2007. Because swimming effort vectors could be computed from the simultaneous fish and flow data, fish behavior could be categorized as passive, swimming against the flow (positively rheotactic), and swimming with the flow (negatively rheotactic). We present bivariate relationships to provide insight into fish responses to particular hydraulic variables that engineers might consider during SFO design. The data indicate potential for this empirical approach of simultaneous water/fish measurements to lead to SFO design guidelines in the future.
D0 Silicon Upgrade: Ladder Removal Stress Analysis
Two different methods will be used to affix ladders to the active bulkhead. Layer 1 and layer 3 ladders will be attached to the active bulkhead using pins. To provide a highly conductive thermal path between these ladders and the cooling channel in the active bulkhead, a small amount of thermally conductive grease will be applied to the bulkhead just prior to ladder installation. Layer 2 and layer 4 ladders will be attached using the same method with the exception that thermally conductive epoxy will be used in lieu of grease. The epoxy is necessary in the outer two layers to maintain barrel rigidity. One major point of concern is the removal of the epoxied ladders. If removal of one of these ladders becomes necessary, it is unclear if the epoxy bond can be broken without damaging the silicon. This report is an attempt to estimate the amount of force necessary to remove a ladder, and the resulting stress that force produces in the silicon. The force necessary to remove an epoxied ladder from a bulkhead is calculated. The resulting silicon stress is also calculated.
Electronic and optical properties of free standing Pt nanowires using localized basis sets
In this paper we have investigated structural, electronic and optical properties of free standing ultrathin NWs of Pt with different topologies using ab-initio SIESTA code. Dimer and ladder chains have found smallest and largest cohesive energy respectively. Electronic band structure and DOS calculations finds more bands and states respectively around Fermi level in ladder topology of Pt chains. It is found that linear and dimer chains act as a good reflector as compared to ladder and zig-zag topologies of Pt chains. Plasmonic frequency of zig-zag and ladder chains are found largest and smallest respectively.
Evolution of the Spin Gap Upon Doping a 2-Leg Ladder
The evolution of the spin gap of a 2-leg ladder upon doping depends upon the nature of the lowest triplet excitations in a ladder with two holes. Here we study this evolution using various numerical techniques for a t-t'-J ladder as the next-near-neighbor hopping t' is varied. We find that depending on the value of t', the spin gap can evolve continuously or discontinuously and the lowest triplet state can correspond to a magnon, a bound magnon-hole-pair, or two separate quasi-particles. Previous experimental results on the superconducting two-leg ladder Sr12Ca2Cu24O41 are discussed.
A mean field theory for the spin ladder system
In the present paper, we propose a mean field approach for spin ladders based upon the Jordan-Wigner transformation along an elaborately ordered path. We show on the mean field level that ladders with even number legs open a energy gap in their low energy excitation with a magnitude close to the corresponding experimental values, whereas the low energy excitation of the odd-number-leg ladders are gapless. It supports the validity of our approach. We then calculate the gap size and the excitation spectra of 2-leg-ladder system. Our result is in good agreement with both the experimental data and the numerical results.
Susceptibility and low temperature thermodynamics of spin-1/2 Heisenberg ladders
The temperature dependence of the uniform susceptibility and the ground state energy of antiferromagnetic Heisenberg ladders with up to 6 legs has been calculated, using the Monte Carlo loop algorithm. The susceptibilities of even-leg-ladders show spin gaps while these of odd-leg-ladders remain finite in the zero temperature limit. For small ratios of intra- to inter-leg couplings, odd-leg-ladders can be mapped at low temperatures to single chains. For equal couplings, the logarithmic corrections at low temperatures increase markedly with the number of legs.
The ladder-type polyheterofluorenes were investigated theoretically by using density functional theory (DFT) to reveal their optical and electronic properties for applications in organic optoelectronic devices. The incorporation of heteroatoms (B, Si, Ge, N, P, O, and S) into the ladder-type highly fused polyfluorene backbone can influence and modify the optoelectronic properties significantly. The functionalization on the heteroatoms allows for facile derivation and incorporation of substitutes to further tune the properties. Small geometry variations between the ground, anionic/cationic, the first excited singlet and triplet states were observed due to the very rigid ladder-type coplanar backbone. Ladder-type polycarbazole was predicted to have the highest HOMO and LUMO energy levels, po...
PitPro 1.1 User's Manual; Pit-tag to SURPH Data Translation Utility, Technical Manual 2003.
This manual describes the use of Program PitPro to convert PIT-tag data files in PTAGIS (PIT Tag Information System, Pacific States Marine Fisheries Commission) to input files ready for survival analysis in Program SURPH 2.1. This utility converts the various PIT-tag detections at the multitude of detector coils within a juvenile bypass or at adult counting windows and ladders into capture histories. The capture histories indicate whether a tagged fish was detected, not detected, or detected and censored at the major hydroprojects in the Columbia Basin. A major update to this program is the inclusion of adult upstream detection histories. Adult detection histories include not only whether the fish was detected or not but also the year of detection for proper adult survival estimation. The SURPH program is a valuable tool for estimating survival and detection probabilities of fish migrating in the Snake and Columbia rivers. Using special input data files, SURPH computes reach-to-reach statistics for any release group passing a system of detection sites. However, PIT-tag data, as available from PTAGIS, comes in a form that is not ready for use as SURPH input. SURPH requires a capture history for each fish. A capture history consists of a series of fields, one for each detection site, that has a code for whether the fish was detected and returned to the river, detected and removed, or not detected. The data, as received from PTAGIS, has one line for each detection with information such as fish identification (id), detection date and time, number of coil hits and detector coil ids, etc. Because an individual fish may be detected at several coils within a detection site as well as at several detection sites, each fish is often represented by multiple lines in the PTAGIS data file. For the PTAGIS data to be usable by SURPH, it must be preprocessed. The data must be condensed down to one line per fish with the relevant detection information from the PTAGIS file represented compactly on each line. In addition, the PTAGIS coil information must be passed through a series of logic algorithms to determine whether or not a fish was returned to the river after detection. Program PitPro was developed to allow the user to properly preprocess the PTAGIS data files for input to program SURPH through a user friendly graphical user interface (GUI). This utility takes PTAGIS data files as input and creates a SURPH data file as well as other output including travel time records, detection date records, and a data error file. PitPro allows a user to download PTAGIS files and easily process the data for use with SURPH. Program PitPro is based on the command line utility CaptHist.
Six-Degree-of-Freedom Sensor Fish Design: Governing Equations and Motion Modeling
The Sensor Fish device is being used at Northwest hydropower projects to better understand the conditions fish experience during passage through hydroturbines and other dam bypass alternatives. Since its initial development in 1997, the Sensor Fish has undergone numerous design changes to improve its function and extend the range of its use. The most recent Sensor Fish design, the three degree of freedom (3DOF) device, has been used successfully to characterize the environment fish experience when passing through turbines, in spill, or in engineered fish bypass facilities at dams. Pacific Northwest National Laboratory (PNNL) is in the process of redesigning the current 3DOF Sensor Fish device package to improve its field performance. Rate gyros will be added to the new six degree of freedom (6DOF) device so that it will be possible to observe the six linear and angular accelerations of the Sensor Fish as it passes the dam. Before the 6DOF Sensor Fish device can be developed and deployed, governing equations of motion must be developed in order to understand the design implications of instrument selection and placement within the body of the device. In this report, we describe a fairly general formulation for the coordinate systems, equations of motion, force and moment relationships necessary to simulate the 6DOF movement of an underwater body. Some simplifications are made by considering the Sensor Fish device to be a rigid, axisymmetric body. The equations of motion are written in the body-fixed frame of reference. Transformations between the body-fixed and interial reference frames are performed using a formulation based on quaternions. Force and moment relationships specific to the Sensor Fish body are currently not available. However, examples of the trajectory simulations using the 6DOF equations are presented using existing low and high-Reynolds number force and moment correlations. Animation files for the test cases are provided in an attached CD. The next phase of the work will focus on the refinement and application of the 6DOF simulator developed in this project. Experimental and computational studies are planned to develop a set of force and moment relationships that are specific to the Sensor Fish body over the range of Reynolds numbers that it experiences. Lab testing of prototype 6DOF Sensor Fish will also allow for refinement of the trajectory simulations through comparison with observations in test flumes. The 6DOF simulator will also be an essential component in tools to analyze field data measured using the next generation Sensor Fish. The 6DOF simulator will be embedded in a moving-machinery computational fluid dynamics (CFD) model for hydroturbines to numerically simulate the 6DOF Sensor Fish.
Distribution of Squalidus chankaensis biwae, Lepomis macrochilus LAFINESQUE, Rhinogobius brunneus, Tribolodon hakonensis and Zacoo platypus within the habitat was strongly influenced by in-situ water velocity. For example, larval fish of Squalidus chankaensis biwa, 6-20mm in SL. preferred swimming a water velocity of 0-2cm/sec at the water surface. Similarly. juvenile fish. 30-40mm in SL. preferred a water velocity of 4-5cm/sec. But the preferred water velocity for adult fish ranged from 5-25cm /sec near the bed of the stream. Similarly. adult fish of Zacco platypus was 40-90cm/sec. The other kinds of fish too. it was measured similar phenomenon. Mostly freshwater fish until juvenile period preferred a water velocity of 0-5cm/sec at the water surface near aquatic plants. but in the beginning of adult fish period, preferred water velocity was made great magnification and fish has habitabled at the near bed-pebbles of swift current. These data are important to the design of good fish habitat. 21 refs., 20 figs., 2 tabs.
Augmented Fish Health Monitoring for Washington Department of Wildlife, 1989 Annual Report.
The augmented fish health monitoring project is funded by the Bonneville Power Administration (BPA) with the mandate to collect fish health data on anadromous fish stocks of the BPA Columbia River Basin in a standardized manner. The project began in 1986 and the data reported here was collected in the fourth year. This segment of the project was carried out by the Washington Department of Wildlife and summarizes fish health findings at anadromous game fish hatcheries in Washington State operated by the BPA. Information gathered to data has provided impetus to alter facility design and management practices for improved fish health through prevention. Treatment efficacy can be better assessed due to the monthly monitoring of fish stocks and insight is being gained into disease prevention and control. The ultimate goal, of course, is to improve fish health for better survival in the wild. Tagged returns at index hatcheries within this project area will provide some indication of the impact of improving fish health on providing greater adult returns as well as an improved product for the fishery. 3 refs., 2 figs., 16 tabs.
We examined the response of fishes to establishment of a new flow regime in lower Putah Creek, a regulated stream in California, U.S.A. The new flow regime was designed to mimic the seasonal timing of natural increases and decreases in stream flow. We monitored fish assemblages annually at six sample sites distributed over approximately 30 km of stream for eight years before and nine years after the new flow regime was implemented. Our purpose was to determine whether more natural stream flow patterns would reestablish native fishes and reduce the abundances of alien (nonnative) fishes. At the onset of our study, native fishes were constrained to habitat immediately (flow regime, native fishes regained dominance across more than 20 km of lower Putah Creek. We propose that the expansion of native fishes was facilitated by creation of favorable spawning and rearing conditions (e.g., elevated springtime flows), cooler water temperatures, maintenance of lotic (flowing) conditions over the length of the creek, and displacement of alien species by naturally occurring high-discharge events. Importantly, restoration of native fishes was achieved by manipulating stream flows at biologically important times of the year and only required a small increase in the total volume of water delivered downstream (i.e., water that was not diverted for other uses) during most water years. Our results validate that natural flow regimes can be used to effectively manipulate and manage fish assemblages in regulated rivers. PMID:22908707
The first six months of the fishery investigations in Libby Reservoir were aimed at developing suitable methodology for sampling physical-chemical limnology, fish food availability, fish food habits, and seasonal distribution and abundance of fish populations. Appropriate methods have been developed for all aspects with minor modification of original proposed methodologies. Purse seining has yet to be tested. Physical-chemical limnologic sampling could be reduced or subcontracted with the U.S. Geologic Survey to allow for more intensive sampling of fish food or fish distribution portions of the investigation. Final sample design will be determined during 1983-84. Future directions of the study revolve around two central issues, the potential for flexibility in reservoir operation and determination of how reservoir operation affects fish populations. Simulated maximum drawdown levels during a 40-year period were controlled by power in seven out of eight years. Drawdowns were generally within 10 feet of the flood control rule curve, however. There may be more flexibility with regards to timing of refill and evacuation. This aspect needs to be evaluated further. Production and availability of fish food, suitability of reservoir habitat, and accessibility of off-reservoir spawning and rearing habitat were identified as components of fish ecology which reservoir operation could potentially impact. Two models based on trophic dynamics and habitat suitabilities were suggested as a framework for exploring the relationship of reservoir operation on the fish community.
Public health benefits and risks of fish consumption: current scientific evidence v. media coverage.
OBJECTIVE: To evaluate if and how the current degree of scientific uncertainty about the safety of fish consumption is incorporated at the media level. DESIGN: We used a dedicated software (TalTac®) to investigate the content of 169 news articles related to 'mercury and fish consumption' that appeared from 1990 to 2010 in the two Italian broadsheets with the highest circulation figures, in order to identify journalistic frames used in the coverage of benefits v. risks associated with fish consumption. Hypotheses were made on how the public might change fish consumption patterns as a result of media coverage. SETTING: Italy. RESULTS: The two newspapers have different agendas in covering the issue. La Repubblica appears to support the view that, besides health benefits, there may be risks associated with fish consumption, while Corriere della Sera emphasizes health benefits more than possible risks. Depending on the preferred information source, the public could: (i) reduce its fish intake; (ii) increase its fish intake; or (iii) become confused about the problem and sceptical towards the media, as a result of conflicting journalistic frames. CONCLUSIONS: The Italian media, in cooperation with scientists, public health nutritionists and dietitians, should place more emphasis on the existence of a few fish species with high to very high Hg levels and relatively low contents of beneficial n-3 fatty acids (e.g. swordfish and shark). This would enable consumers to make more educated purchasing decisions to maximize the benefits of n-3 intake while reducing possible risks from consuming Hg-contaminated fish. PMID:23009805
Context.-The 4-probe, multicolor, fluorescence in situ hybridization (FISH) panel targeting chromosomes 6 and 11 has shown promising sensitivity and specificity in distinguishing between benign nevi and malignant melanoma. Only a few studies have assessed the potential utility of FISH in classification of histologically ambiguous melanocytic lesions. In the United States, this assay is exclusively licensed to NeoGenomics Laboratories (Irvine, California), which provides the technical component and has developed an innovative service (MelanoSITE) allowing pathologists to interpret FISH results using a dedicated Web portal. Thus far, use of MelanoSITE as a diagnostic adjunct in the diagnosis of melanocytic lesions has not, to our knowledge, been reported in the literature. Objective.-To analyze 1.5 years of experience with the MelanoSITE melanoma FISH assay in the evaluation of histologically ambiguous lesions in the context of second opinion and routine dermatopathology practice. Design.-A prospective histologic/FISH correlation study of 140 cases. Results.-Twenty-seven percent of abnormal FISH results were false-positive results because of tetraploidy. After correcting for known false-positive results, all lesions considered atypical nevi showed normal FISH signals. Abnormal FISH signals were reported in 30% of lesions considered histologically borderline and in 48% of lesions in which a diagnosis of melanoma was favored. Conclusions.-Four-probe, multicolor FISH results for melanoma correlate with the microscopic assessments of histologically ambiguous lesions. Pathologists using MelanoSITE must be aware of the high rate of false-positive results from tetraploidy. PMID:22480223
The effects of level of fish oil inclusion in the diet on grass silage intake, and milk yield and composition of dairy cows offered either 5 or 10 kg concentrates/d were evaluated in a ten treatment, partly balanced, changeover design experiment involving 50 cows in early lactation. Concentrates were prepared to provide 0, 150, 300 or 450 g fish oil/cow per d or 300 g fish oil/cow per d from a premix when each animal was offered 5 kg/d. The fish oil was predominantly from herring and mackerel caught in the North Atlantic while the fish oil premix was obtained from a commercial source and used palm kernel expeller as a carrier. Increasing fish oil supplementation decreased silage dry matter intake and the concentrations of milk fat and protein, and increased milk yield and diet digestibility. There were significant interactions between concentrate feed level and level of fish oil for silage intake and milk yield. Other than for the concentrations of milk fat and protein, and 20:4n-6 fatty acids, the source of fish oil did not affect forage intake or animal performance. Fish oil supplementation also decreased the concentrations of milk protein by 0.9 g/kg for each 100 g increase in fish oil supplementation, the depression being similar at each level of concentrate feeding. Supplementing the feed of dairy cows with 450 g fish oil/cow per d decreased the concentration of milk fat by 15 g/kg. This study also showed that feeding dairy cattle with fish oil is an efficient method of increasing eicosapentaenoic acid in the human diet through transfer into milk. PMID:10840669
John Day Fish Passage and Screening; 2002 Annual Report.
This project is necessary to insure that replacement of fish screening devices and fishways meet current NMFS design criteria for the protection of all salmonid life stages. The mission of the fish passage program in Northeast Oregon is to protect and enhance fish populations by assisting private landowners, public landowners, irrigation districts and others by maintaining fish screening devices and fishways. These facilities reduce or eliminate fish loss associated with irrigation withdrawals, and as a result insure fish populations are maintained for enjoyment by present and future generations. Assistance is provided through state and federal programs. This can range from basic technical advice to detailed construction, fabrication and maintenance of screening and passage facilities. John Day screens personnel identified 50 sites for fish screen replacement, and one fish passage project. These sites are located in critical spawning, rearing and migration areas for spring chinook, summer steelhead and bull trout. All projects were designed and implemented to meet current NMFS criteria. It is necessary to have a large number of sites identified due to changes in weather, landowner cooperation and access issues that come up as we try and implement our goal of 21 completed projects.
This study was designed to investigate the protective effects of the active part of Artemisia sacrorum Ledeb. Extract (ASE) against acetaminophen (APAP)-induced hepatotoxicity in mice. As a result, pretreated with ASE prior to the administration of APAP significantly prevented the increases of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and tumor necrosis factor-? (TNF-?) levels in serum, and glutathione (GSH) depletion, malondialdehyde (MDA) accumulation in liver tissue. In addition, ASE prevented APAP-induced apoptosis and necrosis, as indicated by a liver histopathological analysis and DNA laddering. Furthermore, according to the results from Western blot analysis, ASE markedly decreased APAP-induced caspase-3 and -8 protein expressions in mouse livers. All these results suggest that the protective effects of ASE against APAP-induced liver injury may involve mechanisms associated with its inhibitive effects of lipid peroxidation and the down-regulation of TNF-? mediated apoptosis.
Consumers' perceptions of HPP and PEF food products
Purpose - The purpose of this paper is to examine consumer attitudes towards apple juice produced by means of two new processing technologies, high-pressure processing (HPP) and pulsed electric field processing (PEF). Design/methodology/approach - Means-end chain approach is used. Laddering interviews are conducted with consumers in Norway, Denmark, Hungary and Slovakia. Findings - Consumers in this study did recognize and appreciate the benefits that HPP and PEF apple juice have to offer over a juice produced by pasteurization. The respondents in all four countries associated positive consequences with product attributes related to the nutritional value and the taste of the products produced by means of these novel technologies. Also the environmental benefits from processing foods by app...
Recent elliptic curve scalar multiplication algorithms are based on efficient co- Formula Not Shown arithmetics. These arithmetics were initially introduced by Meloni in 2007 where addition of projective points share the same Formula Not Shown -coordinate. The co- Formula Not Shown version algorithms are sufficiently fast and secure against a large variety of implementation attacks. This paper analyses the performance of these algorithms in hardware and then compares them against software and hardware?software co-design environments on FPGA, in terms of speed, memory, power and energy consumption. Specifically, this paper presents a survey and performance comparison of implementations of co- Formula Not Shown versions of the Montgomery ladder and the Joye?s double-add algorithm in an embed...
A wideband RF amplifier for satellite tuners
This paper presents the design and measured performance of a wideband amplifier for a direct conversion satellite tuner. It is composed of a wideband low noise amplifier (LNA) and a two-stage RF variable gain amplifier (VGA) with linear gain in dB and temperature compensation schemes. To meet the system linearity requirement, an improved distortion compensation technique and a bypass mode are applied on the LNA to deal with the large input signal. Wideband matching is achieved by resistive feedback and an off-chip LC-ladder matching network. A large gain control range (over 80 dB) is achieved by the VGA with process voltage and temperature compensation and dB linearization. In total, the amplifier consumes up to 26 mA current from a 3.3 V power supply. It is fabricated in a 0.35-?m SiGe BiCMOS technology and occupies a silicon area of 0.25 mm2.
We developed and evaluated the specificity and sensitivity of a loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne pathogen Salmonella. A pair of outer primers and a pair of inner primers was specially designed for recognizing six distinct sequences on the target invA gene. By the detection system, 241bp target DNA was amplified and visualized as ladder-like pattern of bands on agarose gel within 60min under isothermal conditions at 65degreeC. The detection limit of this LAMP assay was 100fg DNA/tube, while the PCR method was 1pg/tube. Also, the presence of 100ng of non-Salmonella genomic DNA in this LAMP reaction for 1pg Salmonella of target DNA neither adversely affected the amplification efficiency nor generated significant background. In addition...
Challenges in the crystal growth of Li2CuO2 and LiMnPO4
Single crystals of a potential battery material, LiMnPO4, and the spin ladder compound Li2CuO2 are grown under elevated gas pressure in a newly designed vertical floating zone facility with optical heating. Lithium vaporization and its reaction with gases in the growth chamber are principal challenges of the growth process. By applying high argon pressure of 40bar during Travelling Solvent Floating Zone growth of LiMnPO4 the vaporization was reduced by orders of magnitude. The optical radiation, penetrating the transparent melt, affects the crystal/melt interface curvature during the growth. The growth direction was recognized to be decisive for changing the interface curvature from concave to convex and enabling efficient grain selection. By using an Ar/O2 gas mixture in crystal growth of...
Abstract The fragment of the membrane protein M gene with high conservation and specificity of porcine reproductive and respiratory syndrome virus (PRRSV) was chosen to be the target region, according to which six special primers were designed successfully. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to detect the PRRSV by incubation at 65degree for only 45 min with the ladder-like pattern of bands from 182 bp on the agarose gel, and the product specificity was confirmed by RsaI. The result of RT-LAMP could also be visualized directly with the naked eye by adding the intercalating dye Picogreen. The RT-LAMP was identified to detect only the PRRSV in all viruses tested, which demonstrated the high specificity. By using various sample dilutions as templ...
Three dimensional imaging of DNA fragments during electrophoresis using a confocal detector
We have measured the three dimensional distribution of DNA fragments within an electrophoretic band. The measurements were made using a confocal microscope and a photon counting photomultiplier detector. A DNA sequencing standard was loaded into glass microchannel plates containing polyacrylamide gel. The measurements were made by scanning the plates in three dimensions using a mechanical stage under computer control, while electrophoresis was taking place. We found that the distribution of DNA was the same for all the bands measured in the sequencing ladder with an approximate Gaussian distribution along all three axes. These measurements are important to understand what physical forces shape electrophoretic bands confined by a channel and also to aid in the design of high throughput DNA sequencers.
Because of different characteristic features of distribution networks, such as radial structure and high R/X ratio, distribution system load flow analysis differs significantly from transmission systems. Accordingly, a number of methods that are specially designed for the solution of the power flow problem in radial distribution systems are found in the literature. Most of the developed methods are based on the forward/backward sweep processes for the solution of the ladder networks. In this article, various distribution system load flow algorithms, based on the forward/backward sweeps, are reviewed, and their convergence ability is quantitatively evaluated for different loading conditions, R/X ratios, and sub-station voltage levels. Moreover, the effects of static load modeling on the con...
Generation of high current, long duration rectangular pulses
The excitation of the fast pulsed kicker magnets foreseen for the CERN 400 GeV proton synchrotron requires rectangular pulses with a current amplitude of 3000 A to 10000 A, a pulse duration adjustable between 1 and 24 mu sec, and short rise and fall times. These pulses are generated by a LC ladder network discharged with fast switches. Several kinds of switches have been tested: multigap thyratrons of standard design, a composite switch called 'thyragnitron' and made of a normal thyratron by-passed ignitrons, and finally special thyratrons with a second cathode assembly in place of the usual anode. Experimental pulse shapes and results of life tests for these different switches are presented and discussed. (8 refs).
A 10 GHz multiphase LC VCO with a ring capacitive coupling structure
A multiphase LC voltage-controlled oscillator (VCO) with a novel capacitive coupling CL ladder filter structure is proposed in this paper and this 10 GHz eight-phase VCO is applied in clock and data recovery (CDR) circuit for 40 Gb/s optical communications system. Compared with the traditional eight-phase oscillator, this capacitive coupling structure can decrease the number of inductors to half and only of four inductors. The VCO is designed and taped out in TSMC 65 nm CMOS technology. Measurement results show the phase noise is 105.95 dBc/Hz at 1MHz offset from a carrier frequency of 10 GHz. The chip area of VCO is 480 ?m?700 ?m and the VCO core power dissipation is 4.8 mW with the 1.0 V supply voltage.
A 10 GHz multiphase LC VCO with a ring capacitive coupling structure
A multiphase LC voltage-controlled oscillator (VCO) with a novel capacitive coupling CL ladder filter structure is proposed in this paper and this 10 GHz eight-phase VCO is applied in clock and data recovery (CDR) circuit for 40 Gb/s optical communications system. Compared with the traditional eight-phase oscillator, this capacitive coupling structure can decrease the number of inductors to half and only of four inductors. The VCO is designed and taped out in TSMC 65 nm CMOS technology. Measurement results show the phase noise is 105.95 dBc/Hz at 1MHz offset from a carrier frequency of 10 GHz. The chip area of VCO is 480 ?m×700 ?m and the VCO core power dissipation is 4.8 mW with the 1.0 V supply voltage.
Environmental influences on DNA curvature
DNA curvature plays an important role in many biological processes. To study environmentalinfluences on DNA curvature we compared the anomalous migration on polyacrylamide gels ofligation ladders of 11 specifically-designed oligonucleotides. At low temperatures (25 degreesC and below) most of the sequences exhibited a degree of anomalous migration. Increasedtemperature had a significant effect on the anomalous migration (curvature) of some sequencesbut limited effects on others; at 50 degrees C only 1 sequence migrated anomalously. Mg2+ hada strong influence on the migration of certain sequences, whilst spermine enhanced theanomalous migration of a different set of sequences. Sequences with a GGC motif exhibitedgreater curvature than predicted by the presently-used angles for the nearest-neighbour wedgemodel and are especially sensitive to Mg2+. The data have implications for models for DNAcurvature and for environmentally-sensitive DNA conformations in the regulation of geneexpression.
Purpose - The purpose of this paper is to describe a program at Hellmann Worldwide Logistics UK to encourage internal promotions and to award talented employees with deserved recognition. Design/methodology/approach - The paper explains how TalentPool puts ten specially selected employees through a number of key development modules, providing them with the skills to climb the company's career ladder. Findings - The paper lists the seven development modules, in areas including management training, presentation skills, health and safety, finance for non-finance managers and general product training. Reveals that the final test is a presentation before members of the UK board. Coursework is set after each module and marked to help to determine a TalentPool "top achiever". Practical implicatio...
Seismic Analysis for a Crane System
The operation bridge used for an open-pool type research reactor is a crane system with a working deck for the handling of in-pool parts such as fuels, reactor components and reactor utilization facilities. The operation bridge allows operators to access the top of the reactor in the reactor pool and the fuel storage racks in the service pool. The operation bridge contains an operating platform mounted on a truck travelling on rails. Upright members are mounted on the truck to support the upper structure and two hoist monorails. The operation bridge consists of two hoists, upper girder frames, legs, cables, saddle frames, upper deck frames, lower deck frames, and the ladder. Static and dynamic analyses are performed to evaluate the structural integrity for the operation bridge for the required design loadings. The response spectrum analysis is employed as a dynamic analysis method
A planar high-pass filter (HPF) by using cross-couplings in multi-layer structure is proposed in this paper. The HPF consists of parallel plate and gap type capacitors and inductor lines on the bottom conductor. The one block of the HPF has a ladder T-section in the bridge T configuration. The one block HPF is, thus, coarsely designed in the manner of the proto-type HPF and the performance is optimized by circuit simulator. With the gap capacitor adjusted the proposed HPF illustrates the steep slope characteristics near the cut-off frequency by the attenuation pole. In order to improve the stopband performance, the cascaded two block HPF is examined. Its measured results show the good agreement with the simulated ones giving the second attenuation pole by an inductive cross-coupling.
Design technique of broadband CMOS LNA for DC - 11GHz SDR
This paper presents a DC-11GHz CMOS low noise amplifier (LNA) for software-defined radio (SDR). The broadband performance is extended to cover the spectrum from near DC to 11GHz by adopting extra inductors with modified resistive feedback, folded current reuse topology. Bandwidth extension is proposed by inserting pole splitting, interstage and LC ladder inductors. A source follower jointly acts as the buffer stage for broadband output matching and feed-forward path for gain enhancement as well as noise cancellation. Simulation shows power gain of 11 ±4dB and the NF ranging from 1.8 to 3dB in 0.4-11GHz band. The LNA achieves an average IIP3 of -10dBm while consumes only 5.3mW. The proposed broadband LNA is designed in 0.18-µm CMOS process from 1.5V supply.
A study on the goal value for massively multiplayer online role-playing games players
This study examines the goal of value sought by players of the massively multiplayer online role playing games (MMORPGs). We drew on the Means-end Chains (MECs) model frequently used in marketing as a theoretical basis. Soft laddering method was also adopted as a tool for in-depth interviews. Content analysis was used to analyze the ''Attributes-Consequences-Values'' for MMORPGs players, then converted into a hierarchical value map (HVM). The study found that role-playing, interface design, multiplayer gaming, independent play, popularity and virtual pets were the order of game attributes users took into consideration when playing MMORPGs. The consequences benefits for the users were, in order, enhanced interaction, more fun, enhanced efficiency, fantasy fulfillment, winning, novelty, more...
As the homelessness `crisis' in the United States enters a third decade, few are as adversely affected as persons with serious mental illness. Despite recent evidence favoring a `housing first' approach, the dominant `treatment first' approach persists in which individuals must climb a ladder of program requirements before becoming eligible for an apartment of their own.Drawing upon the concept of `ontological security', this qualitative study examines the subjective meaning of `home' among 39 persons who were part of a unique urban experiment that provided New York Citys homeless mentally ill adults with immediate access to independent housing in the late 1990s. The study design involved purposively sampling from the experimental (housing first) group (N=21) and the control (treatment fir...
Abstract Here, we report the identification, purification, characterization and gene cloning of a novel hypersensitive response inducing protein secreted by necrotrophic fungus, Alternaria tenuissima, designated as hypersensitive response inducing protein 1 (Hrip1). The protein caused the formation of necrotic lesions that mimic a typical hypersensitive response and apoptosis-related events including DNA laddering. The protein-encoding gene was cloned by rapid amplification of cDNA ends (RACE) method. The sequence analysis revealed that the cDNA is 495 bp in length and the open reading frame (ORF) encodes for a polypeptide of 163 amino acids with theoretical pI of 5.50 and molecular weight of 17-562.5-Da. Hrip1 induced calcium influx, medium alkalinization, activation of salicylic acid-ind...
High-speed DNA genotyping using microfabricated capillary array electrophoresis chips
Capillary array electrophoresis (CAE) chips have been designed and fabricated with the capacity to rapidly (<160 s) analyze 12 different samples in parallel. Detection of all lanes with 0.3 s temporal resolution was achieved using a laser-excited confocal-fluorescence scanner. The operation and capabilities of these CAE microdevices were first determined by performing electrophoretic separations of pBR322 MspI DNA samples. Genotyping of HLA-H, a candidate gene for the diagnosis of hereditary hemochromatosis, was then performed to demonstrate the rapid analysis of biologically relevant samples. Two-color multiplex fluorescence detection of HLA-H genotypes was accomplished by prelabeling the standard pBR322 MspI DNA ladder with a red emitting bisintercalation dye (butyl TOTIN) and on-column labeling of the HLA-H DNA with thiazole orange. This work establishes the feasibility of using CAE chips for high-speed, high-throughput genotyping. 44 refs., 7 figs.
R and D of energy saving and new energy utilization in Japanese marine engineering
Energy saving measures being studied and implemented in Japan to reduce marine diesel fuel consumption are described. Lower ship speeds, bulb bows, better surface paint, a reaction ladder, low speed diesel engines with long stroke cylinders and superchargers are being tried. Large, low speed, controllable pitch propellers, digital engine control, and waste heat recovery with Rankine cycle generators are other methods under investigation. Blade cooling and reheating are being introduced, and a set of computer controlled maneuverable metal sails undergoing sea tests on a commercial tanker as a fuel saving technique have displayed a 20% fuel savings in tests. A windmill is under design for ship propulsion, involving several vertical airfoils revolving around a vertical axis of rotation. Direct connection to a propeller is being considered, noting that operations to windward would be possible.
Evaluate Habitat Use and Population Dynamics of Lampreys in Cedar Creek, Annual Report 2002.
Pacific lamprey (Lampetra tridentata) in the Columbia River basin have declined to a remnant of their pre-1940s populations and the status of the western brook lamprey (L. richardsoni) and river lamprey (L. ayresi) is unknown. Identifying the biological and ecological factors limiting lamprey populations is critical to their recovery, but little research has been conducted on these species within the Columbia River basin. This ongoing, multi-year study examines lamprey populations in Cedar Creek, Washington, a third-order tributary to the Lewis River. This annual report describes the activities and results of the third year of this project. Adult (n = 62), metamorphosed (n = 76), transforming (n = 4), and ammocoete (n = 315) stages of Pacific and western brook lamprey were examined in 2002. Lampreys were captured using adult fish ladders, lamprey pots, rotary screw traps, and lamprey electrofishers. In addition, fifty-four spawning ground surveys were conducted during which 124 Pacific lamprey and 13 western brook lamprey nests were identified. Stream gradient of spawning grounds were surveyed to better understand spawning habitat requirements.
In less than a decade, zebra mussels have become the latest environmental scourge to plague the North American power industry. Infestations in the Great Lakes region have already reached natural disaster proportions. The invasion shows little sign of subsiding; Michigan's inland waters are the next most likely threatened area. In the southern United States, the mussles' migration has extended about 50 miles deeper than experts had originally predicted. By the year 2000, zebra mussel monitoring and control efforts will cost business and industry $5 billion, according to the federal Aquatic Nuisance Prevention and Control Act of 1990. Estimates of more than $1 million to control mussel fouling are projected for the Great Lakes area alone. While small independent hydropower stations are not as susceptible to zebra mussles as coal or nuclear facilities, there is cause for concern. Infestations can quickly foul hydropower plant components, hampering equipment operation and reducing facility efficiency. In extreme cases, leaving the mussels unchecked can result in stoplog gate flow blockage or false water level gauge readings. Advance prevention is often an effective first-line of defense against this troublesome, rapidly spreading and extremely prolific mollusk. Mussel monitoring efforts should begin a year in advance of when zebra mussels are expected to appear in a given location. Hydropower facility components that come into contact or rely exclusively on raw water are at greatest risk, as are other external components such as embayment walls, screens, trashracks and fish ladders.
New types of mouse centromeric satellite DNAs.
Genomic databases do not contain complete sequences of the centromeric regions. We created a pUC19-based library of DNA fragments from isolated chromocentres of interphase nuclei. In this library we have found major satellite (MaSat) and two new satellite sequences - MS3 and MS4. The computer analysis of MS3 and MS4 sequences by alignment, fragment curved state and search for MAR motifs in comparison with the mouse major and minor satellite (MiSat) DNA has shown them to be new satellite fragments. Southern blot of MS3 and MS4 with total DNA digested by restriction enzymes shows the ladder characteristic of satellite DNA. 2.2% of the total DNA consists of MS3, the monomer of which is 150 bp long. The MS4 monomer is 300 bp long and accounts for 1.6% of the total DNA. On metaphase chromosomes MS3 and MS4 are located at the centromeric region. FISH analysis of L929 nuclei during the cell cycle showed relative positions of MaSat, MiSat, MS3, and MS4. All mapped satDNA fragments except MaSat belong to the outer layer of the chromocentres in the G0/G1 phase. MS3 is likely to be involved in the centromere formation. The mouse genome contains at least four satDNA types: AT-rich (MaSat and MiSat), and CG-rich (MS3 and MS4). PMID:15791408
The micro power station of Verbois; Ouvrage Micro-centrale de Verbois
The policy of the public utilities of the City of Geneva includes the promotion of renewable energy and the conservation of the environment. The erection of the most important hydroelectric power plant in the canton of Geneva at Verbois, on the Rhone River dates back to the years 1938 to 1944. Several retrofits have been made since then, the last dating from 1996 to 1998. In 2000, due to ecological considerations, a fish ladder was added, resulting in a loss of generated power equivalent to a water flow of 2 m{sup 3}/s at a height of 18.35 meters. In order to compensate for this energy loss, a micro hydropower station with a maximum output power of 316 kW was added. It is fitted with a Francis turbine with horizontal axis and fixed opening; the rotation speed is 605 rpm. A reduced power output could be traced back to the deposition of solids on the grids upstream of the water supply duct. The necessary modifications are discussed.
Ichthyophthirius multifiliis infection induces apoptosis in different species of Tilapia.
The parasitic ciliate Ichthyophthirius multifiliis (Ich) is one of the most important protozoan pathogens of freshwater fish worldwide. Examination of 4 species of Tilapia from the River Nile recorded highest rate infection in Oreochromis niloticus, followed by Oreochromis aureus, Sarotherodon galilaeus and Tilapia zilli. By electron microscopy, apoptotic cells collected from Tilapia species infected with "Ich" showed an aggregation on the apical and basal parts of the ciliated and non-ciliated endothelial lining the gill epithelium. Ultrastructural analysis showed that the chromatin in the liver of Ich-infected O. niloticus was highly condensed and massed at the center of the nucleus. UItrastructural analysis of "Ich" infected O. aureus showed that the nuclear membrane of hepatocytes was also shrunken. In S. galilaeus, chromatin was highly condensed, fragmented and massed at the nuclear center, occasionally forming crescent-shaped masses. Ultrastructural of O. niloticus spleen showed that the macrophage number was comparatively high as compared to control. In O. aureus, damaged splenic cell number did not change, and phagocytic capacity of macrophages was not very high. In Ich-infected S. galilaeus, a marked decrease in splenic cells number was seen. Analysis of DNA by agarose gel electrophoresis on gill, liver and spleen cells showed a ladder of DNA fragments in multiple of 180bp in length, pointing to an internucleosomal DNA cleavage. PMID:19795773
Three run-of-river power plants
Three `run-of-river` hydroelectric power plants in the Montreal area in the province of Quebec were described visually and in sound. A run-of-river generating station is one that has no reservoir behind the generating facilities. Instead of a reservoir, the generating station draws its power from the strong flow of the whole river as it passes through the turbines. The first generating station described was the Beauharnois power plant completed in 1963 which became the most powerful generating station in Canada at that time. Today, it ranks fourth after the La Grande complex. In winter, it supplies electricity primarily to the Quebec power system, but between April and November, 90 per cent of its power is destined for export. The Carillon power station on the Ottawa River, the second to be discussed in this videotape presentation, was completed in 1964 with a total generating capacity of 654 MW. Today, it is the tenth largest of its kind in Quebec. The Rivieres des Prairies generating station, the third and last one described was completed in 1930; today it has a generating capacity of 45 MW. Some of the efforts made by Hydro-Quebec to protect and enhance the natural environment were shown in action, including regular removal and recycling of debris at the gateways to the generating stations, construction of fish spawning ladders, and the control of zebra mussels.
This report presents the results of an evaluation of juvenile Chinook salmon (Oncorhynchus tshawytscha) behavior at Cougar Dam on the south fork of the McKenzie River in Oregon in 2010. The study was conducted by the Pacific Northwest National Laboratory for the U.S. Army Corps of Engineers (USACE). The overall goal of the study was to characterize juvenile salmonid behavior and movement patterns in the immediate forebay of the Water Temperature Control (WTC) tower of the dam for USACE and fisheries resource managers use in making decisions about bioengineering designs for long-term structures and/or operations to facilitate safe downstream passage for juvenile salmonids. We collected acoustic imaging (Dual-Frequency Identification Sonar; DIDSON) data from March 1, 2010, through January 31, 2011. Juvenile salmonids (hereafter, called 'fish') were present in the immediate forebay of the WTC tower throughout the study. Fish abundance index was low in early spring (<200 fish per sample-day), increased in late April, and peaked on May 19 (6,039 fish). A second peak was observed on June 6 (2904 fish). Fish abundance index decreased in early June and remained low in the summer months (<100 fish per sample-day). During the fall and winter, fish numbers varied with a peak on November 10 (1881 fish) and a minimum on December 7 (12 fish). A second, smaller, peak occurred on December 22 (607 fish). A univariate statistical analysis indicated fish abundance index (log10-transformed) was significantly (P<0.05) positively correlated with forebay elevation, velocity over the WTC tower intake gate weirs, and river flows into the reservoir. A subsequent multiple regression analysis resulted in a model (R2=0.70) predicting fish abundance (log-transformed index values) using two independent variables of mean forebay elevation and the log10 of the forebay elevation range. From the approximate fish length measurements made using the DIDSON imaging software, the average fish length during early spring 2010 was 214 {+-} 86 mm (standard deviation). From May through early November, the average fish length remained relatively consistent (132 {+-} 54 mm), after which average lengths increased to 295 {+-} 148 mm for mid-November though early December. From mid-December through January the average fish length decreased to 151 {+-} 76 mm. Milling in front of the WTC tower was the most common fish behavior observed throughout the study period. Traversing along the front of the tower, east-to-west and west-to-east, was the next common behavior. The percentage of fish events showing movement from the forebay to the tower or from the tower to the forebay was generally low throughout the spring, summer, and early fall (0 to 30% for both directions combined, March through early November). From mid-November 2010 through the end of the study (January 31, 2011), the combined percentages of fish moving into and out of the tower were higher (25 to 70%) than during previous months of the study. Schooling behavior was most distinct in the spring. Schooling events were present in 30 to 96% of the fish events during that period, with a peak on May 19. Schooling events were also present in the summer, but at lower numbers. With the exception of some schooling in mid-December, few to no schooling events were observed in the fall and winter months. Diel distributions for schooling fish during spring and fall months indicate schooling was concentrated during daylight hours and no schooling was observed at night. However, in December, schooling occurred at night, after midnight, and during daylight hours. Predator activity, most likely bull trout or rainbow trout according to a USACE biologist, was observed during late spring, when fish abundance index and schooling were highest for the year, and again in the fall months when fish events increased from a summer low. No predator activity was observed in the summer, and little activity occurred during the winter months.
We report on our effort from October 1990 through March 1991 to prepare for the evaluation of the juvenile fish bypass facility in the West Extension Irrigation District Canal at Three Mile Falls Dam on the Umatilla River. We also report on our preliminary activities to prepare for future evaluations at Maxwell and Westland diversion dams. A detailed sampling plan was written to guide our efforts in the evaluation process and associated preparatory activities were conducted. In the sampling plan, we developed experimental designs for evaluating the passage of juvenile salmonids through the bypass system including the evaluation at design flow of injury and mortality rates, and passage of juvenile salmonids through and over the screens. We designed and fabricated fish nets for screen leakage tests, and holding facilities for test fish. Modifications to improve evaluation activities were incorporated into the collection facility, and our sampling gear. We designed and fabricated collection systems for the juvenile fish bypass facilities at Maxwell and Westland diversion dams. Preliminary monitoring of system operation was performed at Westland Diversion Dam. We offer recommendations for improving preparations and designs of future evaluations, and also recommend that a detailed evaluation of the Maxwell and Westland juvenile facilities, including evaluation of fish condition and fish passage through or over the screens, be conducted.
Augmented Fish Health Monitoring, 1992 Annual Report.
The Augmented Fish Health Monitoring Project (Project) had its origin, in the mid-1980's, in perceived differences or inconsistencies in fish disease detection, diagnosis and control capabilities between the five conservation agencies rearing and releasing anadromous salmonids for fishery resource management and mitigation purposes in the Columbia River basin. Agency fish health programs varied greatly. Some agencies had personnel, equipment and funding to frequently monitor the health status of both juvenile production fish and adult salmon or steelhead trout at the time of spawning. Other agencies had much smaller programs and limited resources. These differences became better understood when the Pacific Northwest Fish Health Protection Committee developed its Model Fish Health Protection Program including recommendations for standard fish disease detection procedures. Even though some agencies could not immediately attain the goals set by the Model Program it was unanimously adopted as a desirable objective. Shortly thereafter, a multi-party planning group was assembled to help the Bonneville Power Administration (BPA) find ways to improve agency fish health programs and implement measures under the Fish and Wildlife Program of the Northwest Power Planning Council. The planning group assessed existing agency fish health monitoring capabilities, agreed upon satisfactory levels of capability to detect and identify important fish pathogens, and designed a five-year project establishing comparable fish health monitoring capability in each agency. It was strongly believed that such a project would improve the health and quality of the millions of hatchery fish released annually in the Columbia River basin and improve interagency communications and disease control coordination. During 1986 and 1987 BPA individually negotiated five separate contracts with the fishery agencies to standardize fish health monitoring, develop a common data collection and reporting format, evaluate and monitor hatchery water supplies, improve communications and coordination, and provide a mechanism for periodic review. The contract with the U. S. Fish and Wildlife Service was approved on May 31, 1987, the last of the five inter-agency agreements to be signed. This did not delay Service participation with the other agencies because the Service already had many elements of the Project in place under its existing Service-wide fish health policy and implementation plan, The Service fully participated in the Project from mid-1987 through mid-1992. This completion report describes some of the many accomplishments under each of the objectives, benefits accruing from the Project, elements to be sustained by the Service after the Project ended, and Project that were not completed or that pointed out new work that needs to be done.
The validity of the means-end chain model of consumer behaviour
Despite its popularity in consumer research, means-end chain theory suffers from problems of unconfirmed validity: the nomological status of its central construct, the means-end chain, is still unknown. The aim of the research reported here was threefold: (a) to reformulate means-end chain theory in a coherent theoretical framework, (b) to derive falsifiable predictions from the framework, and (c) to test these predictions by established experimental methods. Theoretically, means-end chains can be cast as associative networks with a threelayered structure. Four postulates can be formulated that impose testable restrictions on the layered network structure: hierarchicity, automatic spreading activation, bidirectionality, and self-relevance. The predictions were tested in altogether six experiments. The basic methodology was the same in all experiments. Two sessions were held with each participant. In a pilot session, each participant completed four different laddering tasks. Each task consisted of four different consumer products varying on three different attributes. After the pilot session, the word material that participants had generated in the laddering task was entered into a database. Individualized stimulus sets were then generated from the database for use in the second session. To avoid carry-over effects, the second session was arranged after a long delay. Each participant completed a sequential priming experiment in which singlepresentation lexical decision tasks were used. Experiment 1 (N = 90) was designed to test the hierarchicity and self-relevance postulates. Hierarchicity was tested by examining whether response facilitation effects were higher when primes and targets were directly associated nodes in a means-end chain (attributes and consequences, or consequences and values) than when primes and targets were indirectly associated through a mediator (attributes and values, mediated by consequences). Self-relevance was tested by examining whether response facilitation effects were stronger when primes and targets were taken from a person's own means-end chain (as measured by the laddering method) than when taken from another person's means-end chain or from a standardised word list. In this experiment, only bottom-up priming of means-end chains was investigated.
Sea-Cage Aquaculture, Sea Lice, and Declines of Wild Fish
Abstract: A sea cage, sometimes referred to as a net pen, is an enclosure designed to prevent farm fish from escaping and to protect them from large predators, while allowing a free flow of water through the cage to carry away waste. Farm fish thus share water with wild fish, which enables transmission of parasites, such as sea lice, from wild to farm and farm to wild fishes. Sea lice epidemics, together with recently documented population-level declines of wild salmon in areas of sea-cage farming, are a reminder that sea-cage aquaculture is fundamentally different from terrestrial animal culture. The difference is that sea cages protect farm fish from the usual pathogen-control mechanisms of nature, such as predators, but not from the pathogens themselves. A sea cage thus becomes an unint...
Development of sea lamprey (Petromyzon marinus) larvicides
Larvicides are used to control sea lamprey (Petromyzon marinus) in the Great Lakes. These larvicides are useful because they are more toxic to sea lamprey than fish species found in the same habitat. The lampricides come from two classes of chemical compounds: (1) halonitrophenols, and (2) halonitrosalicylanilides. Selectivity of the larvicides appears to be based on the differences in the ability of sea lamprey larvae and fishes to detoxify and/or excrete the chemicals. Glucuronide conjugation is an important mechanism for detoxification of these larvicides by fish, and selectivity of larvicides may be due to differences in glucuronyl transferase activity between lamprey and fishes. If more detailed information were available on uptake, metabolism, excretion, and the biochemistry and physiology of lamprey as compared to fishes, it might be possible to design chemicals that would be more selective than those now in use.
Towards a quantitative application of real-time PCR technique for fish DNA detection in feedstuffs
A real-time PCR method to detect fish DNA in feedstuffs was developed and optimised. A combination of primers and a Taqman-MGB probe was used to selectively amplify the fish mitochondrial 12S ribosomal RNA gene. Qualitative and also quantitative assessments were performed with different protocols: a relative quantification by a standard curve, and a DCT method, by total plant DNA as endogenous controls. Method specificity was evaluated analysing 40 different tissues (mammalians, avian, fish) and flour samples. Sensitivity was evaluated by LOD (limit of detection) estimation. The designed probe-primers set showed an increased sensitivity compared to previously published PCR end point method, reaching a limit of detection of 0.2pg of fish DNA, and showing to be a robust assay for fish DNA de...
Salmonid fish is one of the allergenic items that are recommended to be labeled in the Japanese allergen-labeling system. This study develops a salmonid-specific polymerase chain reaction (PCR) method. A new primer pair, SKE-F/SKE-R, was designed to specifically detect the salmonid fish gene encoding mitochondrial DNA cytochrome b. Genomic DNAs extracted from 58 kinds of seafood and 11 kinds of processed food were individually subjected to PCR by using the primer pair, and a salmonid-specific fragment of 212 bp was only amplified in the salmonid samples and salmonid-containing processed foods. The detection limit of the PCR method was as low as 0.02 fg/µL of salmonid fish DNA (corresponding to 10 copies). There is no ELISA method for salmonid fish, making our PCR method the only reliable measure for detecting salmonid fish in processed foods.
Presence of fish affects lake use and breeding success in ducks
Several previous studies indicate that presence of fish has negative effects on waterbirds breeding on lakes, owing either to competition for common invertebrate prey or fish predation on ducklings/chicks. However, others have reported results to the contrary and it remains unresolved what factors trigger, inhibit, and modulate fish?waterbird interactions. The present study was designed to test the effect of fish presence per se, with a minimum of variation in possibly confounding environmental variables. Thus, after stratifying for area, depth, altitude, pH, and total phosphorus we compared 13 lakes with and 12 without fish (mainly pike Esox lucius and perch Perca fluviatilis) with respect to (i) general species richness of waterbirds, (ii) species-specific utilization and breeding succes...
Existing evidence indicated that dichlorodiphenyltrichloroethane (DDT)-containing antifouling paints were an important source of DDT residues to mariculture zones. However, the magnitude of the impact on aquafarming environment has remained largely unknown. In the present study, the concentrations of DDT and its metabolites (designated as DDXs) were determined in harbor sediment and antifouling paint samples collected from a typical mariculture zone in South China. Compositional and concentration correlation analyses implicated the DDT-containing antifouling paints for fishing boat maintenance as an important source of DDT in the mariculture zone. The annual emission of DDXs to the study region was estimated at 0.58 tons/yr. Furthermore, a comparison of the expected DDT loadings in pelagic fish and field measurements indicated that fish feed especially trash fish was a major source of DDTs in the fish body. Nevertheless, the use of DDT-containing antifouling paints should be limited to prevent further deterioration in aquafarming environment. PMID:21835519
Prevent mortality and/or injury to all life stages of anadromous and resident fish at irrigation diversions. Obsolete Yakima basin fish screens constructed in the 1930's, 40's, 50's, 60's, and 70's must be replaced or updated to comply with current regional fish screen biological protection criteria adopted by Columbia Basin Fish and Wildlife Authority (CBFWA), Fish Screening Oversight Committee (FSOC) in 1995. This goal is being accomplished through an on-going effort by the Yakima Basin Phase II Technical Work Group (TWG), which is comprised of local, state, federal, tribal and private groups who prioritizes and assign screening projects. Prioritized screening projects are completed through coordination between the Bureau of Reclamations (BOR), who design and permit, and the WDFW who fabricate the screens for these projects.
An experiment was designed to study the effect of dietary quinine hydrochloride (61 mg/kg of fish/day), on the rate of xenoma formation in the gills of Loma salmonae-infected juvenile rainbow trout maintained at a water temperature of 15 degrees C. Almost all (90.9%) control fish had developed xenomas by week 6 post-exposure (PE), but significantly fewer (18.2%) of the medicated fish were similarly affected (P xenomas, but only 57.5% of quinine-treated had xenomas (P Xenoma dissolution and branchitis, two crucial events in the pathogenesis of L. salmonae infection in farm-reared Pacific salmon, were present at week 10 in control fish. In contrast, comparable lesions did not develop in treated fish until week 14. These findings are of potential significance for the control of L. salmonae infection in farmed salmon. PMID:9839206
The potential of a highly concentrated rapeseed protein isolate (RPI) as partial or total fish meal (FM) alternative in diets for turbot (Psetta maxima L.) was evaluated. In a feeding trial 12 experimental tanks of a saltwater recirculation system were stocked with 15 fish each. Fish were organized in triplicate groups and received isonitrogenous and isoenergetic experimental diets with 0, 33, 66, or 100% (designated as control, RPI 33, RPI 66, RPI 100) of FM protein replaced with RPI. Fish were fed over a period of 56days until apparent satiation. Feed intake, feed conversion ratio and growth performance did not significantly vary between fish fed the RPI 33 (129g RPIkg^-^1 diet) and the control diet. The RPI 66 (258g RPIkg^-^1 diet) and the RPI 100 (391g RPIkg^-^1 diet) diets caused redu...
Abstract To study the possible environmental impact of hydraulic cockle-dredging on macrobenthic communities and the environment, a fishing experiment was executed on a tidal mudflat in the Oosterschelde (SW Netherlands) according to a BACI (before-after-control-impact) design. Following the characterization of the initial situation, a part of the mudflat was commercially fished, after which dredged and undredged areas were compared on the basis of macrofauna descriptors and sediment constitution approximately 2-months (short term) and 1-year (mid-long term) after fishing. Whereas a clear reduction of the larger Cerastoderma edule cockles (>23-mm) in the fished areas was found, no effect of dredging on total macrofauna densities or median grain size was observed. No negative effect of fish...
Objective To determine the meiotic segregation in large-headed, multiple-tailed spermatozoa. Design Analysis of sperm nuclei by fluorescence in situ hybridization (FISH). Setting University hospital. Patient(s) A 34-year-old man with 100% morphologically abnormal spermatozoa. Intervention(s) Dual-color FISH for chromosomes 13 and 21 and triple-color FISH for chromosomes X, Y, and 18 were performed. Main Outcome Measure(s) Aneuploidy rates. Result(s) More than 99% of the spermatozoa had abnormal content for chromosomes X, Y, 13, 18, and 21. Diploidy, triploidy, and tetraploidy rates were found to be 18.42%, 6.14%, and 33.99% in triple-color FISH and to be 16.09%, 16.28%, and 38.95% in dual-color FISH. Conclusion(s) Our results and those from other investigators show that large-headed, multi...
THE AQUATIC INVADERS: Marine Management Figuring Fishermen, Fisheries, and Lionfish in The Bahamas
Abstract The lionfish is an enigmatic, beautiful, and invasive marine species in The Bahamas, where the reef ecology is construed as vulnerable while fishermen and invasive fish are seen as primary threats. This article considers fisheries anthropology through recent attempts to incorporate the lionfish into the Bahamian fishery as a commercial fish species, and it explains how the mysterious fish has become symbolic of the creativity and design of contemporary fisheries and fisheries management. Starting with the premise that marine management creatively calls fish, fishermen, and fisheries into being as socially charged objects through conservation-oriented studies of fishing and invasion, the article engages with maritime anthropology, social studies of invasive biology, and multispecie...
The seaward migration of juvenile salmonids was monitored by the National Marine Fisheries Service (NMFS) at Bonneville and John Day Dams on the Columbia River in 1996 The NMFS Smolt Monitoring Project is part of a larger Smolt Monitoring Program (SMP) coordinated by the Fish Passage Center (FPC) for the Columbia Basin Fish and Wildlife Authority. The purpose of the SMP is to monitor the migration of the juvenile salmonid stocks in the Columbia basin and make flow and spill recommendations designed to facilitate fish passage. Data are also used for travel time, migration timing, and relative run size analysis. The purpose of the NMFS portion of the program is to provide the FPC with species and project specific real time data from John Day and Bonneville Dams. Monitoring data collected included: river conditions; total numbers of fish; numbers of fry, adult salmon, and incidental catch; daily and seasonal passage patterns; and fish condition. 10 refs., 16 figs., 5 tabs.
Pike predation affects breeding success and habitat selection of ducks
Summary 1. Fish and ducks often belong to the same local food web, and several studies indicate that there is a general negative effect of fish on breeding ducks. This pattern has so far been addressed mainly within the framework of competition for common invertebrate prey, while predation by large fish as a force behind settlement and abundance patterns in ducks remains largely unknown. This is the first study to address the effect of fish predation on breeding ducks, isolated from that of competition, and the first experiment to explore the ability of ducks to identify and avoid lakes with high risk of fish predation. 2. We used a before-after control-impact design and 11 naturally fishless lakes. Waterfowl on the lakes were surveyed during the breeding season of 2005. Large adult pike (...
Fifty-Five Years of Fish Kills in Coastal Texas
The designation of Texas as a ?hotspot?? for fish mortalities relative to the other 22 coastal US states is of serious concern for scientists, resource managers, and the public alike. We investigated the major sources and causes of fish kills in coastal Texas from 1951 to 2006. During this 55-year period, more than 383 million fish were killed, 72% of which were Gulf menhaden (Brevoortia spp.). We examined the relationships between climate and the physical features of Texas bays and estuaries as well as the consequences of high-density industrialization and urbanization along several coastal centers on fish kills, including the impact of eutrophication, algal blooms (toxic and nontoxic), and hypoxia. Galveston and Matagorda Bays had the highest number of fish kill events and total number o...
CCR Connections - Don’t Throw Out the Packing Materials
Most illustrations of DNA depict a kind of ladder spiraling off into the distance, the ladder being the famous DNA double helix consisting of paired nucleotide bases. Although it has long been known that mammalian DNA is packed very tightly and systematically with specialized proteins into material called chromatin, researchers are only now beginning to appreciate the importance of chromatin structure in gene regulation.
Exponential suppression with four legs and an infinity of loops
The L-loop 4-point ladder diagram of massless phi^3 theory is finite when all 4 legs are off-shell and is given in terms of polylogarithms with orders ranging from L to 2L. We obtain the exact solution of the linear Dyson-Schwinger equation that sums these ladder diagrams and show that this sum vanishes exponentially fast at strong coupling.
In the advent of the installation of a PIT-tag interrogation system in the Cascades Island fish ladder at Bonneville Dam, this report provides guidance on the anticipated precision of in-river survival estimates for returning adult salmonids, between Bonneville and Lower Granite dams, for various levels of system-wide adult detection probability at Bonneville Dam. Precision was characterized by the standard error of the survival estimates and the coefficient of variation of the survival estimates. The anticipated precision of in-river survival estimates for returning adult salmonids was directly proportional to the number of PIT-tagged smolts released and to the system-wide adult detection efficiency at Bonneville Dam, as well as to the in-river juvenile survival above Lower Granite Dam. Moreover, for a given release size and system-wide adult detection efficiency at Bonneville Dam, higher estuarine and marine survival rates also produced more precise survival estimates. With a system-wide detection probability of P{sub BA} = 1 at Bonneville Dam, the anticipated CVs for in-river survival estimate ranged between 9.4 and 20% with release sizes of 10,000 smolts. Moreover, if the system-wide adult detection efficiency at Bonneville Dam is less than maximum (i.e., P{sub BA} < 1), precision of CV {le} 20% could still be attained. For example, for releases of 10,000 PIT-tagged fish a CV of 20% in the estimates of in-river survival for returning adult salmon could be reach with system-wide detection probabilities of 0.2 {le} P{sub BA} {le} 0.6, depending on the tagging scenario.
Program SURPH is the culmination of several years of research to develop a comprehensive computer program to analyze survival studies of fish and wildlife populations. Development of this software was motivated by the advent of the PIT-tag (Passive Integrated Transponder) technology that permits the detection of salmonid smolt as they pass through hydroelectric facilities on the Snake and Columbia Rivers in the Pacific Northwest. Repeated detections of individually tagged smolt and analysis of their capture-histories permits estimates of downriver survival probabilities. Eventual installation of detection facilities at adult fish ladders will also permit estimation of ocean survival and upstream survival of returning salmon using the statistical methods incorporated in SURPH.1. However, the utility of SURPH.1 far exceeds solely the analysis of salmonid tagging studies. Release-recapture and radiotelemetry studies from a wide range of terrestrial and aquatic species have been analyzed using SURPH.1 to estimate discrete time survival probabilities and investigate survival relationships. The interactive computing environment of SURPH.1 was specifically developed to allow researchers to investigate the relationship between survival and capture processes and environmental, experimental and individual-based covariates. Program SURPH.1 represents a significant advancement in the ability of ecologists to investigate the interplay between morphologic, genetic, environmental and anthropogenic factors on the survival of wild species. It is hoped that this better understanding of risk factors affecting survival will lead to greater appreciation of the intricacies of nature and to improvements in the management of wild resources. This technical report is an introduction to SURPH.1 and provides a user guide for both the UNIX and MS-Windows{reg_sign} applications of the SURPH software.
Fish passage mitigation of impacts from hydroelectric power projects in the United States
Obstruction of fish movements by dams continues to be the major environmental issue facing the hydropower industry in the US. Dams block upstream migrations, which can cut off adult fish form their historical spawning grounds and severely curtail reproduction. Conversely, downstream-migrating fish may be entrained into the turbine intake flow and suffer turbine-passage injury or mortality. Hydroelectric projects can interfere with the migrations of a wide variety of fish. Maintenance, restoration or enhancement of populations of these species may require the construction of facilities to allow for upstream and downstream fish passage. The Federal Energy Regulatory Commission (FERC), by law, must give fish and wildlife resources equal consideration with power production in its licensing decisions, must be satisfied that a project is consistent with comprehensive plans for a waterway (including fisheries management plans), and must consider all federal and state resource agency terms and conditions for the protection of fish and wildlife. As a consequence, FERC often requires fish passage mitigation measures as a condition of the hydropower license when such measures are deemed necessary for the protection of fish. Much of the recent research and development efforts of the US Department of Energy`s Hydropower Program have focused on the mitigation of impacts to upstream and downstream fish passage. This paper descries three components of that effort: (1) a survey of environmental mitigation measures at hydropower sites across the country; (2) a critical review of the effectiveness of fish passage mitigation measures at 16 case study sites; and (3) ongoing efforts to develop new turbine designs that minimize turbine-passage mortality.
Recent studies have demonstrated reproductive problems in white sucker (Catostomus commersoni) exposed to bleached kraft pulp mill effluent (BKME) at Jackfish Bay on Lake Superior. These fish exhibit delayed sexual maturity, reduced gonadal size, reduced secondary sexual characteristics, and circulating steroid levels depressed relative to those of reference populations. The present studies were designed to evaluate sites in the pituitary-gonadal axis of prespawning white sucker affected by BKME exposure. At the time of entry to the spawning stream, plasma levels of immunoreactive gonadotropin (GtH)-II (LH-type GtH) in male and female white sucker were 30- and 50-fold lower, respectively, than the levels in fish from a reference site. A single intraperitoneal injection of D-Arg6, Pro9N-Et sGnRH (sGnRH-A, 0.1 mg/kg) increased plasma GtH levels in male and female fish at both sites, although the magnitude of the response was greatly reduced in BKME-exposed fish. Fish at the BKME site did not ovulate in response to sGnRH-A, while 10 of 10 fish from the reference site ovulated within 6 hr. Plasma 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) levels were depressed in BKME-exposed fish and unlike fish at the reference site, failed to increase in response to sGnRH-A. Testosterone levels in both sexes and 11-ketostestosterone levels in males were elevated in fish from the reference site but were not further increased by GnRH treatment. In contrast, BKME-exposed fish exhibit a transitory increase in testosterone levels in response to the GnRH analog. In vitro incubations of ovarian follicles obtained from fish at the BKME site revealed depressed basal secretion of testosterone and 17,20 beta-P and reduced responsiveness to the GtH analog human chorionic gonadotropin and to forskolin, a direct activator of adenylate cyclase.
Clinicopathologic Characteristics of HER2 FISH-ambiguous Breast Cancer at a Single Institution.
BACKGROUND:: The typical algorithm for human epidermal growth factor-2 (HER2) testing is immunohistochemistry (IHC), followed by reflex HER2 fluorescence in situ hybridization (FISH) for HER2 IHC-ambiguous (2+) cases. At our institution, HER2 FISH testing is initially performed as part of routine breast cancer testing, with HER2 FISH-ambiguous (HER2:CEP17 ratio, 1.8 to 2.2) cases reflexed to HER2 IHC. This provides a unique dataset for lesions that may not routinely undergo FISH testing. The clinicopathologic characteristics of HER2 FISH-ambiguous cases are described. DESIGN:: The electronic pathology database in our institution was searched for HER2 FISH-ambiguous cases from 2007 to December 2011. Review of clinical and pathologic characteristics was performed. RESULTS:: Sixty cases from 60 patients were reported as HER2 FISH ambiguous. Reflex HER2 IHC testing was performed on all 60 cases, of which 26 were HER2 IHC negative (0 to 1+), 18 were HER2 IHC ambiguous (2+), and 16 were HER2 IHC positive (3+). Of the 46 HER2 FISH-ambiguous patients with available clinical records, 13 (32%) pursued anti-HER2 treatment (10 IHC 3+, 1 IHC 2+, 2 IHC 0 to 1+). All were grade II or III ductal carcinomas, with 1 grade III metaplastic carcinoma. CONCLUSIONS:: Reflex HER2 IHC testing after initially ambiguous HER2 FISH testing provides definitive HER2 status in a majority of cases (70%). However, a substantial percentage (30%) of HER2 FISH-ambiguous cases is also HER2 IHC ambiguous, suggesting an intermediate HER2 biology. Most HER2 FISH-ambiguous patients who received trastuzumab were HER2 IHC 3+, grade III, and had associated high-grade ductal carcinoma in situ. Although not statistically significant and with only minimal follow-up, no recurrences have occurred in those patients treated with trastuzumab (P=0.5754). PMID:23108020
During 1999-2002 we determined whether size at release and release site influenced emigration success and survival of hatchery steelhead smolts raised at Dworshak National Fish Hatchery and released into the Clearwater River drainage. We marked 4,500 smolts each year with Passive Integrated Transponder Tags (PIT-tags) which enabled us to track emigration and estimate survival through mainstem Snake and Columbia river dams. Hatchery steelhead raised in System I freshwater were significantly smaller than those raised in warmer System II re-use water (196 mm, 206 mm, 198 mm and 201 mm System I; 215 mm, 213 mm, 206 mm and 209 mm System II). However, there was no significant difference in detection rates to mainstem observation sites between the two groups (65%, 58%, 78% and 55% System I; 69%, 59%, 74% and 53% System II). Survival estimates to Lower Granite Dam were also not significant between the two groups (72%, 81%, 80% and 77% System I; 77%, 79%, 77%, and 72% System II). Smolts less than 180 mm FL were less likely to be detected than larger smolts. Hatchery steelhead smolts released into Clear Creek, the South Fork Clearwater River and the Clearwater River at Dworshak National Fish Hatchery had significantly different lengths each year, but there was no discernible pattern due to random egg takes and rearing systems. Detection rates to mainstem observation sites for smolts released into Clear Creek were significantly less than the other two groups in all years except 2002 (62%, 57%, 71%, and 57% Clear Creek; 68%, 63%, 73% and 61% South Fork Clearwater River; 70%, 59%, 78% and 55% Clearwater River). However, survival rates to Lower Granite Dam were not significantly different (73%, 65%, 78%, and 77% Clear Creek; 79%, 72%, 79% and 76% South Fork Clearwater River; 81%, 76%, 80% and 83% Clearwater River). Similar to the size at release group, smolts less than 180 mm FL were less likely to get detected than larger smolts. Smolts from both size at release and release site groups that were mature at tagging rarely migrated downstream. If smolts migrated they did it in the same year they were released, as less than 0.02% were observed migrating the second year. We sampled the Clearwater River, North Fork Clearwater River, Bedrock Creek, Big Canyon Creek, Cottonwood Creek, Jacks Creek and the Dworshak National Fish Hatchery adult ladder to collect residual hatchery steelhead. We PIT-tagged and released 3,651 hatchery steelhead and collected 645 hatchery steelhead for coded wire tags. Most residual hatchery steelhead were caught within 4 rkm of Dworshak National Fish Hatchery. Hatchery steelhead sampled in the North Fork Clearwater River and the Dworshak Hatchery adult ladder were significantly larger than those sampled in the Clearwater River and lower tributaries in all years except 2001 (205 mm, 205 mm, 223 mm and 238 mm North Fork Clearwater River; 190 mm, 182 mm, 226 mm and 189 mm Clearwater River). Of the hatchery steelhead we PIT-tagged, only 12% were observed at downstream observation sites. Most migrants were tagged in the Clearwater River (91%) and were smaller than hatchery steelhead that were tagged but were not detected. Most migrants were detected in the same year they were tagged, but 14% held over and migrated in the second year after tagging. We documented migration outside of the normal window, as one detection occurred on October 31 at Lower Granite Dam. We recaptured 130 individual hatchery steelhead that we had tagged during sampling. Over 77% of the recaptures were within one km of where they were tagged, and 67% of the recaptures were tagged in the North Fork Clearwater River and the Dworshak Hatchery adult ladder. We calculated a mean growth rate of 0.27 mm/day for fish we recaptured. For those hatchery steelhead we PIT-tagged, the proportion of males was 13%, the rest we could not ascertain gender. All the males were precocious. Over 97% of the coded-wire tag recoveries came from hatchery steelhead released at Dworshak National Fish Hatchery. The Contribution group (random egg take and rearing system) comprised 44% of the recoveries, followed by 32% System I and 24% System II. The mean length of the Contribution group was significantly smaller than the other two groups in all years except 2001 (173 mm, 191 mm, 209 mm and 197 mm Contribution; 210 mm, 206 mm, 205 mm and 228 mm System I; 226 mm, 216 mm, 213 mm and 225 mm System II). Males made up 81% of the recoveries, and their maturity ranged from 0% to 100%. The Contribution group had significantly more males than the other two groups in 1999 and 2001.
We have investigated the importance of the relative phase (?) between the THz field and the optical field, which is used to monitor the interband absorption, and cw optical absorption spectrum in biased semiconductor superlattices. All of the absorption spectra have Stark ladder structures and the peaks of the Stark ladders are changed for the different phases. Especially, it is demonstrated that the changes of the Stark ladders from those ?=0 occur periodically in four ladders for ?=? and in eight ladders for ?=??2 in the absence of dynamical localization. The cw optical absorption spectrum, which is obtained as an average over the phases by using short pulse technique, have no gain peaks. Because this procedure leads to a cancellation of regions of negative absorption which may occur in the coherent THz-driven system.
On a spectrum of the t-J model on a deformed lattice ``necklace ladder''
The effective Hamiltonians describing the lowest part of the energy spectrum in the t-J model on a lattice ``necklace ladder'' with a weak interaction of elementary cells are derived within the framework of perturbation theory and the formalism of cyclic spin permutations. It is shown that in a model of ``necklace ladder'' there are no magnetic polarons which are observed in similar rectangular ladders. A nonmonotonic dependence of the ground state spin S0 of finite ladder segments, containing L unit cells and N = 3L-1 electrons, on the value of the exchange parameter J is shown numerically. In particular, for a cycling segment with four unit cells for 0 cycling segments of the lattice ``necklace ladder'' containing 3L-1 electrons, for even L an exact separation of charge and spin variables in the corresponding t-J model is demonstrated.
Assembly procedure for the silicon pixel ladder for PHENIX silicon vertex tracker
The silicon vertex tracker (VTX) will be installed in the summer of 2010 to enhance the physics capabilities of the Pioneering High Energy Nuclear Interaction eXperiment (PHENIX) experiment at Brookhaven National Laboratory. The VTX consists of two types of silicon detectors: a pixel detector and a strip detector. The pixel detector consists of 30 pixel ladders placed on the two inner cylindrical layers of the VTX. The ladders are required to be assembled with high precision, however, they should be assembled in both cost and time efficient manner. We have developed an assembly bench for the ladder with several assembly fixtures and a quality assurance (Q/A) system using a 3D measurement machine. We have also developed an assembly procedure for the ladder, including a method for dispensing adhesive uniformly and encapsulation of bonding wires. The developed procedures were adopted in the assembly of the first pixel ladder and satisfy the requirements.
Augmented Fish Health Monitoring for Washington Department of Wildlife, 1988 Annual Report.
The augmented fish health monitoring project is funded by the Bonneville Power Administration with the mandate to collect fish health data on anadromous fish stocks of the Columbia River Basin in a standardized manner. The project began in 1986 and the data reported here was collected in the third year. This segment of the project was carried out by the Washington Department of Wildlife and summarizes fish health findings at anadromous game hatcheries in Washington State operated by the BPA. Information gathered to date has provided impetus to alter facility design and management practices for improved fish health through prevention. Treatment efficacy can be better assessed due to the monthly monitoring of fish stocks and insight is being gained into disease prevention and control. The ultimate goal, of course, is to improve fish health for better survival in the wild. Tagged returns at index hatcheries within this project area will indicate the impact of improving fish health on providing greater adult returns as well as an improved product for the fishery. 2 refs., 3 figs., 15 tabs.
Studies were conducted to describe current habitat conditions in the White River basin above White River Falls and to evaluate the potential to produce anadromous fish. An inventory of spawning and rearing habitats, irrigation diversions, and enhancement opportunities for anadromous fish in the White River drainage was conducted. Survival of juvenile fish at White River Falls was estimated by releasing juvenile chinook and steelhead above the falls during high and low flow periods and recapturing them below the falls in 1983 and 1984. Four alternatives to provide upstream passage for adult salmon and steelhead were developd to a predesign level. The cost of adult passage and the estimated run size of anadromous fish were used to determine the benefit/cost of the preferred alternative. Possible effects of the introduction of anadromous fish on resident fish and on nearby Oak Springs Hatchery were evaluated. This included an inventory of resident species, a genetic study of native rainbow, and the identification of fish diseases in the basin. This volume contains appendices of habitat survey data, potential production, resident fish population data, upstream passage designs, and benefit/cost calculations. (ACR)
The goal of this project is to prevent mortality and/or injury to all life stages of anadromous and resident fish at irrigation diversions. Obsolete Yakima basin fish screens constructed in the 1930's, 40's, 50's, 60's, and 70's must be replaced or updated to comply with current regional fish screen biological protection criteria adopted by Columbia Basin Fish and Wildlife Authority (CBFWA), Fish Screening Oversight Committee (FSOC) in 1995. This goal is being accomplished through an on-going effort by the Yakima Basin Phase II Technical Work Group (TWG), which is comprised of local, state, federal, tribal and private groups who prioritizes and assign screening projects. Prioritized screening projects are completed through coordination between the Bureau of Reclamations (BOR), who design and permit, and the WDFW who fabricate the screens for these projects. The following is the 2004 fish screening facility construction schedule as proposed by the Bureau of Reclamation Design Group (USBR) and Fish Passage Technical Work Group (TWG).
Evaluation of trawls for monitoring and harvesting fish populations in Lake Oahe, South Dakota
Trawls of various designs and sizes were compared to evaluate their use for monitoring and harvesting fish populations in Lake Oahe. Catches of a 10.7-m semiballoon trawl, selected to monitor changes in the fish populations from 1965 to 1970, showed a downward trend in the abundance of all species, except walleye; the decline was largest in the lower area of the reservoir with a slight increase in the upper area. Of two trawl designs tested, semiballoon trawls captured more fish per hour than high-rise trawls, and a 15.9-m semiballoon trawl with a 3.8-cm mesh cod end captured fish at the highest and most consistent rate. The size and species composition of fish caught in small-mesh trawls differed from those caught in trap nets. Trawl catches were too small to recommend or warrant their use as a commercial fishing gear, but the use of both small mesh trawls and trap nets should improve accuracy in monitoring fish populations in this reservoir.
A Study to Determine the Biological Feasibility of a New Fish Tagging System : Annual Report 1984.
The potential of the Passive Integrated Transponder (PIT) tag for marking salmonids was evaluated. In Phase I, sham PIT tags were injected into the body cavity of juvenile salmonids. Tagged fish ranged in weight from 0.8 to 43.9 g. Observations based on six tests, ranging in time from 19 to 99 days, indicated that the test site for injecting the tag was along the mid-ventral line in the area of the pectoral or pelvic fins. Tag retention of 99% (n = 400) and survival of 99% were achieved. Minimal tissue response to the tag was noted in both tag locations. In Phase II, a PIT tag detector system designed to detect and record the passage of juvenile salmonids was evaluated. By restricting the rate at which fish entered the monitoring tunnel to one fish per second, detection averaged 94.3% compared to 86.6% for multiple fish entry. In Phase III, a monitoring system designed to detect and decode adult fish tagged with a PIT tag was evaluated. The average detection and proper decoding of these tagged fish was 94.4% for 211 fish. Improvements in the detection system are recommended.
Optimisation of baited remote underwater video sampling designs for estuarine fish assemblages
Baited remote underwater video stations (BRUVS) are used for monitoring fish assemblages and assessing management effectiveness in reef environments but are infrequently used in estuaries. A review of the BRUVS literature found that most adopted sampling designs from other studies were rarely designed from pilot studies. This potentially compromises their value for monitoring natural and anthropogenic variation. The aims of this study were: (i) to assess the suitability of BRUVS for sampling fishes in estuarine habitats (seagrass beds and unvegetated sediments) and (ii) to develop an optimal and cost effective sampling methodology for each habitat. Fishes in both habitats were sampled independently using BRUVS with soak times of 30, 60, 90min (n=4). Thirty five species of fishes were recor...
Identifying habitats important to vulnerable life stages of reef fish is an important goal for the effective design and management of Marine Protected Areas (MPAs). However, data on habitat requirements for most fish species are limited. Using a benthic habitat map that characterized reef habitats on the island of Hawaii , we examined ontogenetic patterns of habitat use by each of the life stages of endemic (i.e., Ctenochaetus strigosus; Ctenochaetus hawaiiensis), non-targeted species (i.e., Acanthurus nigrofuscus; Chromis agilis; Thallasoma duperrey; Paracirrhites arcatus) and targeted aquarium fish species (Zebrasoma flavescens; C. strigosus; C. hawaiiensis; Chaetodon multicinctus) in relation to the effectiveness of existing MPAs designed to replenish aquarium fishes targeted by the aqu...
Cage design and stocking density are important aspects of aquaculture farm design, therefore understanding how fish behave at different stocking densities is critical information for farm managers. In this study, high resolution acoustic telemetry was used to investigate the swimming behavior of adult Atlantic cod, Gadus morhua, that were stocked at four densities (5, 10, 25, and 45 kg/m3). Acoustic tags were placed into the abdominal cavity of five fish per density treatment so their swimming behavior could be continuously monitored throughout the study. An array of hydrophones made it possible to calculate the position of each fish in three dimensions, at -2-5 sec intervals, for 4-30 d. Three underwater cameras were used to obtain additional data about the distribution of fish in the cag...
The environmental impacts of three different queen scallop (Aequipecten opercularis) fishing gears
The negative impact of demersal fishing gears on the marine environment may be mitigated by utilizing less damaging fishing gears. Within this context three queen scallop fishing gears were tested for their catch efficiencies and their environmental impact on benthos: a traditional 'Newhaven' dredge, a new dredge design with a rubber lip instead of the traditional teeth as its main new design feature and an otter trawl. Both, the new dredge and the otter trawl showed high catches and relatively low by-catches. Catches made with the traditional dredge were lower and contained larger amounts of non-target species. Both dredges primarily caught invertebrate species, while by-catches of the otter trawl were dominated by demersal fish. The impact of these gears on the benthic biota demonstrated...
ObjectiveTo prove the ability to distinguish between balanced and normal chromosomes in embryos from a translocation carrier. DesignCase report. SettingAcademic center for reproductive medicine. Patient(s)Woman with a balanced translocation causing Alagille syndrome seeking preimplantation genetic diagnosis (PGD). Intervention(s)Blastocyst biopsy for PGD. Main Outcome Measure(s)Consistency of 3 methods of embryo genetic analysis (real-time polymerase chain reaction, single nucleotide polymorphism [SNP] microarray, and fluorescence in situ hybridization [FISH]) and normalcy in the newborn derived from PGD. Result(s)PGD was applied to 48 embryos. Real-time polymerase chain reaction, SNP microarray, and FISH demonstrated 100% consistency, although FISH failed to detect aneuploidies observed b...
Acrylamide (ACR) is a known industrial toxic chemical that produce neurotoxicity characterized by progressive neuronal degeneration. This study was designed to investigate the protective effect of fish oil on ACR-induced neuronal damage in Wistar rats. ACR enhances the production of reactive oxygen species and potentially affects brain. ACR administered rats showed increased levels of lipid peroxidative product, protein carbonyl content, hydroxyl radical and hydroperoxide which were significantly modulated by the supplementation of fish oil. The activities of enzymic antioxidants and levels of reduced glutathione were markedly lowered in ACR-induced rats; fish oil treatment augmented these antioxidant levels in cortex. Free radicals generated during ACR administration reduced the activitie...
Design and testing of a fish bone separator machine
A fish bone separator for species like carp (Cyprinus carpio) was designed, built, and tested. The development resulted in a new class of fish bone separator, smaller in size and output but capable of processing hard-boned fish up to 3kg in weight. A description of the developed machine, its working principles, and a comparison with the existing rubber band industrial machines is included. A full-scale prototype was evaluated by comparison with the existing rubber band machine technology. Better colour and texture and 13% higher processing yield were obtained for the developed machine. In addition, this new machine was well accepted by local fishermen.
Coral Reef Habitats as Surrogates of Species, Ecological Functions, and Ecosystem Services
Abstract: Habitat maps are often the core spatially consistent data set on which marine reserve networks are designed, but their efficacy as surrogates for species richness and applicability to other conservation measures is poorly understood. Combining an analysis of field survey data, literature review, and expert assessment by a multidisciplinary working group, we examined the degree to which Caribbean coastal habitats provide useful planning information on 4 conservation measures: species richness, the ecological functions of fish species, ecosystem processes, and ecosystem services. Approximately one-quarter to one-third of benthic invertebrate species and fish species (disaggregated by life phase; hereafter fish species) occurred in a single habitat, and Montastraea-dominated foreree...
The contribution under consideration contains the appendices 2 to 4 to the environmental research plan of the Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (Berlin, Federal Republic of Germany) on ''Hydropower and Water Framework Directive''. Appendix 2 reports on the fundamentals for the design of fish bypass facilities, fish protection and fish migration facilities, minimum outflow in discharge lines, water ecologic evaluation, determination of less generation of hydroelectric power plants. Appendix 3 contains illustrations. Appendix 4 presents an extract from the final report.
Through multiple alignment analysis of mitochondrial tRNA-Thr and tRNA-Phe sequences from 161 fishes, new universal primers specially targeting the entire mitochondrial control region were designed. This new primer set successfully amplified the expected PCR products from various kinds of marine fish species, belonging to various families, and the amplified segments were confirmed to be the control region by sequencing. These primers provide a useful tool to study the control region diversity in economically important fish species, the possible mechanism of control region evolution, and the functions of the conserved motifs in the control region.
Mycobacteria isolated from Chesapeake Bay fish
Abstract Mycobacteriosis in fish can result in ulcers, emaciation, and in some cases death. Mycobacteria have been previously isolated from a variety of Chesapeake Bay fish species, and the current study was designed to identify potential host specificity and location fidelity of mycobacterial isolates. Mycobacteria were isolated from wild fish of the Chesapeake Bay collected from the Upper Bay, the Choptank River, Herring Bay, the Chicamacomico River, the Pocomoke River and the Potomac River in 2003-2006. Mycobacterial isolates were recovered from striped bass, Morone saxatilis, Atlantic menhaden, Brevoortia tyrannus, white perch, Morone americana, summer flounder, Paralichthys dentatus, spot, Leiostomus xanthurus, largemouth bass, Micropterus salmoides, channel catfish, Ictalurus punctat...
The Sensor Fish - Making Dams More Salmon-Friendly
This article describes the Sensor Fish, an instrument package that travels through hydroelectric dams collecting data on the hazardous conditions that migrating salmon smolt encounter. The Sensor Fish was developed by Pacific Northwest National Laboratory with funding from DOE and the US Army Corps of Engineers and has been used at several federal and utility-run hydroelectric projects on the Snake and Columbia Rivers of the US Pacific Northwest. The article describes the evolution of the Sensor Fish design and provides examples of its use at McNary and Ice Harbor dams.
Spawning Distribution of Fall Chinook Salmon in the Snake River : Annual Report 1998.
In 1998 data was collected on the spawning distribution of the first adult fall chinook salmon to return from releases of yearling hatchery fish upriver of Lower Granite Dam. Yearling fish were released at three locations with the intent of distributing spawning throughout the existing habitat. The project was designed to use radio-telemetry to determine if the use of multiple release sites resulted in widespread spawning.
Vertical slot fishways are widely used and are generally very effective in ensuring fish passage. The appropriate design of a vertical slot fishway depends on an interplay between hydraulic and biological variables, since the hydrodynamic properties of the fishway must meet the requirements of the fish species for which it is intended. This paper presents a tool designed to analyze the efficiency of a fishway model according to biological criteria. The application can be used either in the analysis of an existing fishway or in the design process of a new one. It allows the user to choose between a wide range of geometric dimensions of the pools and the slot, and between different fish species. If it is being used as a design tool, it provides feasible fishway models sorted according to the...
This paper summarizes the current state of the knowledge of fish hearing, i.e., what is known about fish response to sound, and provides a starting place for investigation into the use of sonic behavior modification to help solve pressing problems in the maintenance and recovery of anadromous and resident fish stocks of the Columbia River Basin. The challenge for scientists and engineers is to understand (1) how and what fish hear, and (2) how to use this knowledge to influence fish behavior or to factor fish sensory systems and behavior into the design of fish protection devices at power-production and water-control facilities. The sections that follow are a studied response to this challenge. In many respects, the search for effective and economical ways to modify fish behavior without physical intervention is one of the grails of fish management, particularly (although not exclusively), relative to electric power production. Within the Columbia River Basin and elsewhere, water is put to many uses not conducive to the health of indigenous fish. Irrigation, impoundment, and hydropower production frequently modify the riverine environment to the detriment of indigenous stocks while creating productive environments for competing or predatory species. The mechanisms of impact are numerous. Water project managers, when faced with the need to reduce impacts of their operations on fish health, generally attempt to divert fish from high-risk to lower-risk regions or manage the environment in such a way as to increase the survival prospects of impacted species. Almost without exception, risk reduction is accomplished by using physical barriers of one form or another. Unfortunately, physical barriers at water projects have several drawbacks, e.g., reducing available water flow for energy production and incurring high maintenance costs. In addition, recent studies (Sale et al. 1991) have documented that once these barriers are installed, they are rarely evaluated for effectiveness relative to design goals and/or regulatory mandates, and even less rarely monitored throughout the life of their use. All in all, there is interest--if not enthusiasm--across the spectrum, from project owners to regulators, in finding alternatives to physical barriers such that risks to fish health can be reduced during their encounter with water-control facilities.
Native fishes of the upper Colorado River basin (UCRB) have declined in distribution and abundance due to habitat degradation and interactions with normative fishes. Consequently, monitoring populations of both native and nonnative fishes is important for conservation of native species. We used data collected from Muddy Creek, Wyoming (2003-2004), to compare sample size estimates using a random and a fixed-site sampling design to monitor changes in catch per unit effort (CPUE) of native bluehead suckers Catostomus discobolus, flannelmouth suckers C. latipinnis, roundtail chub Gila robusta, and speckled dace Rhinichthys osculus, as well as nonnative creek chub Semotilus atromaculatus and white suckers C. commersonii. When one-pass backpack electrofishing was used, detection of 10% or 25% changes in CPUE (fish/100 m) at 60% statistical power required 50-1,000 randomly sampled reaches among species regardless of sampling design. However, use of a fixed-site sampling design with 25-50 reaches greatly enhanced the ability to detect changes in CPUE. The addition of seining did not appreciably reduce required effort. When detection of 25-50% changes in CPUE of native and nonnative fishes is acceptable, we recommend establishment of 25-50 fixed reaches sampled by one-pass electrofishing in Muddy Creek. Because Muddy Creek has habitat and fish assemblages characteristic of other headwater streams in the UCRB, our results are likely to apply to many other streams in the basin. ?? Copyright by the American Fisheries Society 2006.
Efficacy of a benthic trawl for sampling small-bodied fishes in large river systems
We conducted a study from 1998 to 2001 to determine the efficacy of a benthic trawl designed to increase species detection and reduce the incidence of zero catches of small-bodied fishes. We modified a standard two-seam slingshot balloon trawl by covering the entire trawl with a small-mesh cover. After completing 281 hauls with the modified (Missouri) trawl, we discovered that most fish passed through the body of the standard trawl and were captured in the cover. Logistic regression indicated no noticeable effect of the cover on the catch entering the standard portion of the modified trawl. However, some fishes (e.g., larval sturgeons Scaphirhynchus spp. and pallid sturgeon S. albus) were exclusively captured in the small-mesh cover, while the catch of small-bodied adult fish (e.g., chubs Macrhybopsis spp.) was significantly improved by use of the small-mesh cover design. The Missouri trawl significantly increased the number and species of small-bodied fishes captured over previously used designs and is a useful method for sampling the benthic fish community in moderate- to large-size river systems.
A preliminary survey of fish protection devices either in use or proposed for water intake structures was conducted for the purpose of assessing their potential for reducing impingement and entrainment. All the designs examined can be divided into two basic categories: behavioral screening systems and physical screening systems. The behavioral screening devices rely upon the ability of fish to sense artificial stimuli and respond by swimming away from hazardous areas. These systems are of little or no value in protecting planktonic fish eggs, larvae, and disoriented, heat-shocked, or lethargic adult fishes. Many of the physical screening devices, on the other hand, require the impingement of organisms against a screen before they can be removed from the intake system, thus subjecting survival. Some of the designs incorporate both behavioral and physical sceening concepts. Six devices were selected for further consideration based on their potential or demonstrated effectiveness in reducing impingement and entrainment losses at a variety of intake situations. The structures evaluated were modified vertical traveling screens, louvers, angled vertical traveling screens, horizontal traveling screens, center-flow screens, and wedge-wire screens. Since some of these intake structures represent new concepts, few laboratory or in situ biological studies have been carried out. For others, actual reductions in fish losses have been demonstrated. The design features and status of biological testing is discussed for each device, and an evaluation of their fish protection potential is presented.
Modeling of multiple stocks and programs for master planning and feasibility studies
Public hatchery facilities increasingly respond to recreational fishing demands for larger catchable salmonids and to production programs that require a variety of species. The result of this tendency is to require more complexity in hatchery design and operations involving the simultaneous rearing of several species that results in overlapping production schedules for crops of fish that carryover from 1 year to the next. This paper presents a highly accessible spreadsheet based computer simulation model for use by culturists, designers, and program planners in designing hatchery facilities with these expanded program demands. It addresses trade offs between budget constraints, stocking objectives, available water resources, and production technologies. The model is structured so that all ...
Sustainable design options for the US Fish & Wildlife Prairie Learning Center
US Fish & Wildlife received a grant from the National Institute for Science and Technology to demonstrate sustainable design at the Walnut Creek Wildlife Refuge and Prairie Learning Center in Prairie City, Iowa. Nine different options were investigated. In reviewing the options, US Fish & Wildlife chose those solutions that demonstrated the practice of sustainable design at this facility, rather than opting for solutions which served primarily as demonstrations. A combination of four of the options were recommended for the grant. The options combined daylighting, specification of more sustainable building materials, energy-efficient system design, and the use of biofuels.
Shelters and their use by fishes on fringing coral reefs.
Coral reef fish density and species richness are often higher at sites with more structural complexity. This association may be due to greater availability of shelters, but surprisingly little is known about the size and density of shelters and their use by coral reef fishes. We quantified shelter availability and use by fishes for the first time on a Caribbean coral reef by counting all holes and overhangs with a minimum entrance diameter ?3 cm in 30 quadrats (25 m(2)) on two fringing reefs in Barbados. Shelter size was highly variable, ranging from 42 cm(3) to over 4,000,000 cm(3), with many more small than large shelters. On average, there were 3.8 shelters m(-2), with a median volume of 1,200 cm(3) and a total volume of 52,000 cm(3) m(-2). The number of fish per occupied shelter ranged from 1 to 35 individual fishes belonging to 66 species, with a median of 1. The proportion of shelters occupied and the number of occupants increased strongly with shelter size. Shelter density and total volume increased with substrate complexity, and this relationship varied among reef zones. The density of shelter-using fish was much more strongly predicted by shelter density and median size than by substrate complexity and increased linearly with shelter density, indicating that shelter availability is a limiting resource for some coral reef fishes. The results demonstrate the importance of large shelters for fish density and support the hypothesis that structural complexity is associated with fish abundance, at least in part, due to its association with shelter availability. This information can help identify critical habitat for coral reef fishes, predict the effects of reductions in structural complexity of natural reefs and improve the design of artificial reefs. PMID:22745664
Snake River Spring/Summer Chinook Captive Broodstock Rearing and Research, 2001 Annual Report.
In 1995, the National Marine Fisheries Service (NMFS), in cooperation with the Idaho Department of Fish and Game (IDFG), the Oregon Department of Fish and Wildlife (ODFW), and the Bonneville Power Administration (BPA) established captive broodstock programs to aid in the recovery of Snake River spring/summer chinook salmon (Oncorhynchus tshawytscha) listed as endangered under the U.S. Endangered Species Act (ESA). These programs were intended to provide safety nets for Salmon and Grande Ronde River Basins spring/summer chinook salmon stocks. They also provide a basis of examining the efficacy of captive rearing and captive breeding programs as tools for recovering listed salmonid populations. In years when no or few naturally produced fish return from the sea, captive fish and their progeny can be used to maintain populations in these two Snake River Basin tributaries. The NMFS facility at Manchester, WA, provides the crucial seawater environment needed to culture anadromous salmonids during the marine phase of their life cycle. At the Manchester Research Station, the fish are cultured in 6.1m diameter circular tanks housed in a fully enclosed and secure building. The tanks are supplied with seawater that has been processed to eliminate most marine pathogens. The fish are fed a commercially prepared diet and held at densities and loading rates designed to maximize fish quality. When fish begin to mature, they are transferred to ODFW or IDFG freshwater facilities in Oregon and Idaho for final maturation. The states then release the mature fish (Idaho) or their progeny (Oregon) back into their native Snake River tributary waters in restoration efforts. In FY 2001, NMFS cultured 1996, 1997, 1998, and 1999 broodyear fish at its Manchester Facility. This report addresses program activities from September 1, 2000 to August 31, 2001.
Coral reef habitats as surrogates of species, ecological functions, and ecosystem services.
Habitat maps are often the core spatially consistent data set on which marine reserve networks are designed, but their efficacy as surrogates for species richness and applicability to other conservation measures is poorly understood. Combining an analysis of field survey data, literature review, and expert assessment by a multidisciplinary working group, we examined the degree to which Caribbean coastal habitats provide useful planning information on 4 conservation measures: species richness, the ecological functions of fish species, ecosystem processes, and ecosystem services. Approximately one-quarter to one-third of benthic invertebrate species and fish species (disaggregated by life phase; hereafter fish species) occurred in a single habitat, and Montastraea-dominated forereefs consistently had the highest richness of all species, processes, and services. All 11 habitats were needed to represent all 277 fish species in the seascape, although reducing the conservation target to 95% of species approximately halved the number of habitats required to ensure representation. Species accumulation indices (SAIs) were used to compare the efficacy of surrogates and revealed that fish species were a more appropriate surrogate of benthic species (SAI = 71%) than benthic species were for fishes (SAI = 42%). Species of reef fishes were also distributed more widely across the seascape than invertebrates and therefore their use as a surrogate simultaneously included mangroves, sea grass, and coral reef habitats. Functional classes of fishes served as effective surrogates of fish and benthic species which, given their ease to survey, makes them a particularly useful measure for conservation planning. Ecosystem processes and services exhibited great redundancy among habitats and were ineffective as surrogates of species. Therefore, processes and services in this case were generally unsuitable for a complementarity-based approach to reserve design. In contrast, the representation of species or functional classes ensured inclusion of all processes and services in the reserve network. PMID:18477024
The Grand Coulee Dam was constructed in 1939 without a fish ladder, which eliminated steelhead (Onchorhynchus mykiss), chinook salmon (O. twshwastica), coho salmon (O. kisutch) and sockeye salmon (O. nerka) from returning to approximately 1,835 km (1,140 miles) of natal streams and tributaries found in the upper Columbia River Drainage in the United States and Canada. The Pacific Northwest Electric Power Planning and Conservation Act of 1980 gave the Bonneville Power Administration (BPA), the authority and responsibility to use its legal and financial resources, 'to protect, mitigate, and enhance fish and wildlife to the extent affected by the development and operation of any hydroelectric project of the Columbia River and its tributaries. This is to be done in a manner consistent with the program adopted by the Northwest Power Planning Council (NWPPC), and the purposes of the Act' (NWPPC, 1987). With the phrase 'protect, mitigate and enhance', Congress signaled its intent that the NWPPC's fish and wildlife program should do more than avoid future hydroelectric damage to the basin's fish and wildlife. The program must also counter past damage, work toward rebuilding those fish and wildlife populations that have been harmed by the hydropower system, protect the Columbia Basin's fish and wildlife resources, and mitigate for harm caused by decades of hydroelectric development and operations. By law, this program is limited to measures that deal with impacts created by the development, operation and management of hydroelectric facilities on the Columbia River and its tributaries. However, off-site enhancement projects are used to address the effects of the hydropower system on fish and wildlife (NWPPC 1987). Resident game fish populations have been established in Franklin D. Roosevelt Lake, the reservoir behind Grand Coulee Dam, since the extirpation of anadromous fish species. The resident game fish populations are now responsible for attracting a large percentage of the recreational visits to the region. An increase in popularity has placed Lake Roosevelt fifth amongst the most visited State and Federal parks in Washington. Increased use of the reservoir prompted amplified efforts to enhance the Native American subsistence fishery and the resident sport fishery in 1984 with hatchery supplementation of rainbow trout (O. mykiss) and kokanee salmon (O. nerka). This was followed by the formation of the Spokane Tribal Lake Roosevelt Monitoring Project (LRMP) in 1988 and later by formation of the Lake Roosevelt Data Collection Project in 1991. The Lake Roosevelt Data Collection Project began in July 1991 as part of the BPA, Bureau of Reclamation, and U.S. Army Corps of Engineers System Operation Review process. This process sought to develop an operational scenario for the federal Columbia River hydropower system to maximize the in-reservoir fisheries with minimal impacts to all other stakeholders in the management of the Columbia River. The Lake Roosevelt Monitoring/Data Collection Program (LRMP) is the result of a merger between the Lake Roosevelt Monitoring Program (BPA No. 8806300) and the Lake Roosevelt Data Collection Project (BPA No. 9404300). These projects were merged in 1996 forming the Lake Roosevelt Monitoring Program (LRMP), which continues the work historically completed under the separate projects. The LRMP has two main goals. The first is to develop a biological model for Lake Roosevelt that will predict in-reservoir biological responses to a range of water management operational scenarios, and to develop fisheries and reservoir management strategies accordingly. The model will allow identification of lake operations that minimize impacts on lake biota while addressing the needs of other interests (e.g. flood control, hydropower generation, irrigation, and downstream resident and anadromous fisheries). Major components of the model will include: (1) quantification of entrainment and other impacts to phytoplankton, zooplankton and fish caused by reservoir drawdowns and low water retention times; (2) quantification of seasonal distributions, standing crop, and habitat use of fish food organisms; (3) examination of variations in fish growth and abundance in relation to reservoir operations, prey abundance and predator/prey relationships; and (4) quantification of habitat alterations due to hydrooperations. The second goal of the LRMP is to evaluate the impacts of hatchery kokanee salmon and rainbow trout on the ecosystem and to determine stocking strategies that maximize angler harvest and return of adult kokanee salmon to egg collection facilities. Major tasks of the hatchery evaluation portion of the project include conducting a year round reservoir wide creel survey, sampling the fishery during spring, summer and fall via electro-fishing and gillnet surveys, and collecting information on diet, growth, and age composition of various fish species in Lake Roosevelt.
The Northwest Power Planning Council (Council) receives and reviews proposals to mitigate for fish and wildlife losses and refers approved measures to Bonneville Power Administration (BPA) for funding. The Northwest Power Act (Act) calls on the Council to include measures in its Columbia River Basin Fish and Wildlife Program (Program) to address system-wide fish and wildlife losses. The Act further states that the Council may include in its Program measures that provide off-site mitigation--mitigation physically removed from the hydro project(s) that caused the need to mitigate. The Program includes a goal ''to recover and preserve the health of native resident fish injured by the hydropower system, where feasible, and, where appropriate, to use resident fish to mitigate for anadromous fish losses in the system.'' Among those recommended measures are off-site mitigation for losses of anadromous fisheries including the measure under analysis in this Coeur d'Alene Tribe Trout Production Facility Master Plan, proposed by the Coeur d'Alene Tribe. To meet the need for off-site mitigation for anadromous fish losses in the Columbia River Basin in a manner consistent with the objectives of the Council's Fish and Wildlife Program, the Coeur d'Alene Tribe is proposing that the BPA fund the design, construction, operations and maintenance of a trout production facility on the Coeur d'Alene Indian Reservation. Measures for establishing a Coeur d'Alene fish production facility have been a part of the Council's Program since 1987. The Coeur d'Alene Tribe Trout Production Facility is intended to rear and release westslope cutthroat trout into rivers and streams with the express purpose of increasing the numbers of fish spawning, incubating and rearing in the natural environment. It will use the modern technology that hatcheries offer to overcome the mortality resulting from habitat degradation in lakes, rivers, and streams after eggs are laid in the gravel. Supplementation of native fish stocks in conjunction with effective habitat restoration will be the primary means of achieving these biological goals. Overarching goals for the program include: (1) Protection, mitigation, and enhancement of Columbia River Basin native resident fish resources. (2) Develop, increase, and/or reintroduce natural spawning populations of westslope cutthroat trout into reservation waters. (3) Provide both short and long-term harvest opportunities for the reservation community. (4) Sustain long-term fitness and genetic integrity of targeted fish populations. (5) Keep ecological and genetic impacts to non-targeted fish populations to a minimum.
Individual operational plans for 1993 are provided for the Abernathy Salmon Culture Technology Center, Carson National Fish Hatchery, Dworshak National Fish Hatchery, Eagle Creek National Fish Hatchery, Entiat National Fish Hatchery, Hagerman National Fish Hatchery, Kooskia National Fish Hatchery, Leavenworth National Fish Hatchery, Little White Salmon National Fish Hatchery, Spring Creek National Fish Hatchery, Warm Springs National Fish Hatchery, Willard National Fish Hatchery, and the Winthrop National Fish Hatchery.
Hydroacoustic Evaluation of Juvenile Salmonid Passage and Distribution at Detroit Dam, 2011
Pacific Northwest National Laboratory evaluated juvenile salmonid passage and distribution at Detroit Dam (DET) on the North Santiam River, Oregon for the U.S. Army Corps of Engineers (USACE) to provide data to support decisions on long-term measures to enhance downstream passage at DET and others dams in USACE’s Willamette Valley Project. This study was conducted in response to regulatory requirements necessitated by the listing of Upper Willamette River Spring Chinook salmon (Oncorhynchus tshawytscha) and Upper Willamette River steelhead (O. mykiss) as threatened under the Endangered Species Act. The goal of the study was to provide information of juvenile salmonid passage and distribution at DET from February 2011 through February 2012. The results of the hydroacoustic study provide new and, in some cases, first-ever data on passage estimates, run timing, distributions, and relationships between fish passage and environmental variables at the dam. This information will inform management decisions on the design and development of surface passage and collection devices to help restore Chinook salmon populations in the North Santiam River watershed above DET. During the entire study period, an estimated total of 182,526 smolt-size fish (±4,660 fish, 95% CI) passed through turbine penstock intakes. Run timing peaked in winter and early spring months. Passage rates were highest during late fall, winter and early spring months and low during summer. Horizontal distribution for hours when both turbine units were operated simultaneously indicated Unit 2 passed almost twice as much fish as Unit 1. Diel distribution for smolt-size fish during the study period was fairly uniform, indicating fish were passing the turbines at all times of the day. A total of 5,083 smolt-size fish (± 312 fish, 95% CI) were estimated passed via the spillway when it was open between June 23 and September 27, 2011. Daily passage was low at the spillway during the June-August period, and increased somewhat in September 2011. When the spillway was operated simultaneously with the turbines, spillway efficiency (efficiency is estimated as spillway passage divided by total project passage) was 0.72 and effectiveness (fish:flow ratio—proportion fish passage at a route (e.g., spillway) divided by proportion water through that route out of the total project) was 2.69. That is, when the spillway was open, 72% of the fish passing the dam used the spillway and 28% passed into the turbine penstocks. Diel distribution for smolt-size fish at the spillway shows a distinct peak in passage between mid-morning and mid-afternoon and low passage at night. We estimated that 23,339 smolt-size fish (± 572 fish, 95% CI) passed via the Regulating Outlet (RO) when it was open from October 29 through November 12, 2011, January 2-6, and January 20 through February 3, 2012. During the October–November period, RO passage peaked at 1,086 fish on November 5, with a second peak on November 7 (1,075 fish). When the RO was operated simultaneously with the turbines, RO efficiency was 0.33 and effectiveness was 0.89. In multiple regression analyses, a relatively parsimonious model was selected that predicted the observed fish passage data well. The best model included forebay temperature at depth, forebay elevation, total discharge, hours of daylight, and the operation period. The vertical distribution of fish in the forebay near the face of the dam where the transducers sampled showed fish were generally distributed throughout the water column during all four operational periods. During the refill and full pool periods, vertical distribution was bi-modal with surface-layer and mid-water modes. Patterns for day and night distributions were variable. Fish were distributed above and below the thermocline when it was present (full pool and drawdown periods).
The Willamette Falls Hydroelectric Power Dam, operated by Portland General Electric (PGE), is located on the Willamette River, Oregon. The Project site consists of T.W. Sullivan Power Plant and a 2,950-ft-long spillway located on the top of the Willamette Falls Dam. As part of the effort of protection and enhancement of environmental resources, a flow control structure at the dam was proposed to improve the flow field and enhance the downstream juvenile fish passage in the region just upstream of the forebay (pre-forebay). The flow in the pre-forebay of Willamette Falls Dam is affected by the complex geometry and bathymetry, powerhouse flow, fish ladder flow and the spillway around the dam. The expectation was that the flow would be sensitive to the proposed flow control structures and could be modified to enhance downstream migration. In this study, a three-dimensional, free-surface hydrodynamic model (EFDC) was developed for the pre-forebay region of Willamette Falls to evaluate the feasibility of the proposed alternative and its effect on the flow field in two different flow regimes (low and high river flow), as well as to assess the hydraulic capacity of flow control structures. One of the key challenges in this modeling study was to properly specify the free open boundary conditions along the 2,950-feet-long spillway. In this study, a pressure boundary condition based on hydraulic head rating curves was applied to the free spillway boundary. The numerical model was calibrated with ADP velocity measurements at 17 stations for the existing low flow condition. Good agreements between model results and measured data were obtained, indicating the successful application of pressure boundary condition on the free spillway boundary. The calibrated model was applied to simulate the flow field and free surface elevation in the high flow region near the control flow structures under different alternative conditions. The model results were used to evaluate the effectiveness of flow control structure alternative for downstream fish passage. The model was also used to estimate the hydraulic capacity based on the water surface head drops upstream of the structures. This model application demonstrated that a free surface coastal model can be used successfully to examine free surface hydraulic problems near high velocity regions upstream of spillways at dams.
Willamette Oxygen Supplementation Studies : Annual Report 1994.
Hydropower development and operations in the Columbia River basin have caused the loss of 5 million to 11 million salmonids. An interim goal of the Northwest Power Planning Council is to reestablish these historical numbers by doubling the present runs from 2.5 million adult fish to 5.0 million adult fish. This increase in production will be accomplished through comprehensive management of both wild and hatchery fish, but artificial propagation will play a major role in the augmentation process. The current husbandry techniques in existing hatcheries require improvements that may include changes in rearing densities, addition of oxygen, removal of excess nitrogen, and improvement in raceway design. Emphasis will be placed on the ability to increase the number of fish released from hatcheries that survive to return as adults. Rearing density is one of the most important elements in fish culture. Fish culturists have attempted to rear fish in hatchery ponds at densities that most efficiently use the rearing space available. Such efficiency studies require a knowledge of cost of rearing and the return of adults to the fisheries and to the hatchery.
IgE-mediated allergic reaction to seafood is a common cause of food allergy including anaphylactic reactions. Parvalbumin, the major fish allergen, has been shown to display IgE cross-reactivity among fish species consumed predominantly in Europe and the Far East. However, cross-reactivity studies of parvalbumin from fish species widely consumed in the Southern hemisphere are limited as is data relating to immunological and molecular characterisation. In this study, antigenic cross-reactivity and the presence of oligomers and isomers of parvalbumin from five highly consumed fish species in Southern Africa were assessed by immunoblotting using purified parvalbumin and crude fish extracts. Pilchard (Sardinops sagax) parvalbumin was found to display the strongest IgE reactivity among 10 fish-allergic consumers. The cDNA sequence of the beta-form of pilchard parvalbumin was determined and designated Sar sa 1.0101 (accession number FM177701 EMBL/GenBank/DDBJ databases). Oligomeric forms of parvalbumin were observed in all fish species using a monoclonal anti-parvalbumin antibody and subject's sera. Isoforms varied between approximately 10-13 kDa. A highly cross-reactive allergenic isoform of parvalbumin was identified and sequenced, providing a successful primary step towards the generation of a recombinant form that could be used for diagnostic and potential therapeutic use in allergic individuals. PMID:19616851
Yakima Basin Fish Passage Project, Phase 2
Implementation of the Yakima Basin Fish Passage Project -- Phase 2 would significantly improve the production of anadromous fish in the Yakima River system. The project would provide offsite mitigation and help to compensate for lower Columbia River hydroelectric fishery losses. The Phase 2 screens would allow greater numbers of juvenile anadromous fish to survive. As a consequence, there would be higher returns of adult salmon and steelhead to the Yakima River. The proposed action would play an integral part in the overall Yakima River anadromous fish enhancement program (fish passage improvement, habitat enhancement, hatchery production increases, and harvest management). These would be environmental benefits associated with implementation of the Fish Passage and Protective Facilities Phase 2 Project. Based on the evaluation presented in this assessment, there would be no significant adverse environmental impacts if the proposed action was carried forward. No significant adverse environmental effects have been identified from construction and operation of the Yakima Phase 2 fish passage project. Proper design and implementation of the project will ensure no adverse effects will occur. Based on the information in this environmental analysis, BPA's and Reclamation's proposal to construct these facilities does not constitute a major Federal action that could significantly affect the quality of the human environment. 8 refs., 4 figs., 6 tabs.
Existing evidence indicated that dichlorodiphenyltrichloroethane (DDT)-containing antifouling paints were an important source of DDT residues to mariculture zones. However, the magnitude of the impact on aquafarming environment has remained largely unknown. In the present study, the concentrations of DDT and its metabolites (designated as DDXs) were determined in harbor sediment and antifouling paint samples collected from a typical mariculture zone in South China. Compositional and concentration correlation analyses implicated the DDT-containing antifouling paints for fishing boat maintenance as an important source of DDT in the mariculture zone. The annual emission of DDXs to the study region was estimated at 0.58 tons/yr. Furthermore, a comparison of the expected DDT loadings in pelagic fish and field measurements indicated that fish feed especially trash fish was a major source of DDTs in the fish body. Nevertheless, the use of DDT-containing antifouling paints should be limited to prevent further deterioration in aquafarming environment. - Highlights: > Use of antifouling paints in boat maintenance was deemed a main source of DDTs. > The majority of antifouling paint-derived DDTs was sequestered in sediment. > Fish feed has remained the main input source of DDTs in fish body. - The potential impact from the use of DDT-containing antifouling paints on aquafarming environment is examined.
A retrospective on hydroacoustic assessment of fish passage in Alaskan rivers
The Alaska Department of Fish and Game (ADFG) has enumerated fish stocks in rivers for over 30 years using a variety of acoustic technologies including single-, dual-, and split-beam sonar. Most recently, ADFG has evaluated a relatively new sonar technology at several sites in Alaska to determine its applicability to counting migrating fish in rivers. The new system, called a Dual frequency IDentification SONar (DIDSON), is a high-definition imaging sonar designed and manufactured by the University of Washington's Applied Physics Lab for military applications such as diver detection and underwater mine identification. Results from experiments conducted in 2002-2004 indicate that DIDSON provides significant improvements in our ability to detect, track, and determine the direction of travel of migrating fish in rivers. One of the most powerful uses of the DIDSON has been to combine its camera-like images of fish swimming behavior with corresponding split-beam data. These linked datasets have allowed us to evaluate the effects of fish orientation and swimming behavior on echo shape parameters that have proven useful in the classification of certain fish species.
In this report, the National Marine Fisheries Service (NMFS), in collaboration with the Bonneville Power Administration (BPA), the Washington State Department of Fish and Wildlife (WDFW), and the US Fish and Wildlife Service (USFWS), presents research findings and guidelines for development and evaluation of innovative culture techniques to increase postrelease survival of hatchery fish. The Natural Rearing Enhancement System (NATURES) described in this report is a collection of experimental approaches designed to produce hatchery-reared chinook salmon (Oncorhynchus tshawytscha) that exhibit wild-like behavior, physiology, and morphology. The NATURES culture research for salmonids included multiple tests to develop techniques such as: raceways equipped with cover, structure, and natural substrates to promote development of proper body camouflage coloration; feed-delivery systems that condition fish to orient to the bottom rather than the surface of the rearing vessel; predator conditioning of fish to train them to avoid predators; and supplementing diets with natural live foods to improve foraging ability. The underlying assumptions are that NATURES will: (1) promote the development of natural cryptic coloration and antipredator behavior; (2) increase postrelease foraging efficiency; (3) improve fish health and condition by alleviating chronic, artificial rearing habitat-induced stress; and (4) reduce potential genetic selection pressures induced by the conventional salmon culture environment. A goal in using NATURES is to provide quality fish for rebuilding depleted natural runs.
Abstract in english Haematological changes in ascorbic acid deficient fishes, observed by some authors, awoked the interest in the haematological response of pacu, Piaractus mesopotamicus. The present work studied the influence of different levels of ascorbic acid (vitamin C) in the ration on the haematological parameters of pacu. Fishes at the beginning of the experiment, presented average body weight 8.64±1.62g and 6.15±0.33cm standard length, were fed with diets containing 0. 50, 100 an (more) d 200mg ascorbic acid/kg dry ration. The present work was developed for a period of 24 weeks at the Fish Nutrition Laboratory of Aquaculture Center of Universidade Estadual Paulista (Unesp), Jaboticabal (São Paulo). The fishes were distributed by the completely randomized design in 20 aquaria of 100 liters capacity with six animals in each and five fold treatment. Condutivity, alcalinity. pH and oxigen were measured weekly and temperature daily. The blood colect was done in 60 anesthetized fishes with 50mg MS-222/1. The results suggests that 50mg ascorbic acid/kg dry ration improved the mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and decreased haematocril in unsupplemented fishes, although not differing significantly (P>0.05). The erytrocyte diameter indicated the presence of macrocytic cells in unsupplemented fishes (P
Ising phases of Heisenberg ladders in a magnetic field
We examine the influence of weak anisotropic interactions on the T=0 phase diagram of the frustrated two-leg Heisenberg ladder, a well-studied spin model exhibiting integer and fractional magnetization plateaux separated by gapless incommensurate states. We find that the Dzyaloshinskii--Moriya coupling may substantially modify the phase diagram so that the half-integer plateau and the surrounding gapless phases merge into a single Ising-ordered phase breaking the translational symmetry of the lattice. A different Ising order is found for a weakly frustrated ladder. Implications for experimental ladder and dimer systems are discussed.
Exact results for the thermal and magnetic properties of strong coupling ladder compounds
We investigate the thermal and magnetic properties of the integrable su(4) ladder model by means of the quantum transfer matrix method. The magnetic susceptibility, specific heat, magnetic entropy and high field magnetization are evaluated from the free energy derived via the recently proposed method of high temperature expansion for exactly solved models. We show that the integrable model can be used to describe the physics of the strong coupling ladder compounds. Excellent agreement is seen between the theoretical results and the experimental data for the known ladder compounds (5IAP)$_2$CuBr$_4
Magnetization of undoped 2-leg S = 1/2 spin ladders in La4Sr10Cu24O41
Magnetization data of single crystalline La4Sr10Cu24O41 are presented. In this compound, doped spin chains and undoped spin ladders are realized. The magnetization, at low temperatures, is governed by the chain subsystem with a finite interchain coupling which leads to short range antiferromagnetic spin correlations. At higher temperatures, the response of the chains can be estimated in terms of a Curie-Weiss law. For the ladders, we apply the low-temperature approximation for a S=1/2 2-leg spin ladder by Troyer et al.
Magnon-Hole Scattering and Charge Order in $Sr_{14-x}Ca_xCu_{24}O_{41}$
The magnon thermal conductivity $\\kappa_{\\mathrm{mag}}$ of the hole doped spin ladders in $\\rm Sr_{14-x}Ca_xCu_{24}O_{41}$ has been investigated at low doping levels $x$. The analysis of $\\kappa_{\\mathrm{mag}}$ reveals a strong doping and temperature dependence of the magnon mean free path $l_{\\mathrm{mag}}$ which is a local probe for the interaction of magnons with the doped holes in the ladders. In particular, this novel approach to studying spin degrees of freedom shows that charge ordering of the holes in the ladders leads to a freezing out of magnon-hole scattering processes.
Effects of interladder couplings in the trellis lattice
Strongly correlated models on coupled ladders in the presence of frustration, in particular the trellis lattice, are studied by numerical techniques. For the undoped case, the possibility of incommensurate peaks in the magnetic structure factor at low temperatures is suggested. In the doped case, our main conclusion for the trellis lattice is that by increasing the interladder coupling, the balance between the magnetic energy in the ladders and the kinetic energy in the zig-zag chains is altered leading eventually to the destruction of the hole pairs initially formed and localized in the ladders.
Inelastic neutron scattering from the spin ladder compound (VO)2P2O7
We present results from an inelastic neutron scattering experiment on the candidate Heisenberg spin ladder vanadyl pyrophosphate, (VO)2P2O7. We find evidence for a spin-wave excitation gap of E_{gap} = 3.7\\pm 0.2 meV, at a band minimum near Q=0.8 A^{-1}. This is consistent with expectations for triplet spin waves in (VO)2P2O7 in the spin-ladder model, and is to our knowledge the first confirmation in nature of a Heisenberg antiferromagnetic spin ladder.
Pressure dependence of superconductivity in doped two-leg ladder cuprates
Within the kinetic energy driven superconducting mechanism, the effect of the pressure on superconductivity in doped two-leg ladder cuprates is studied. It is shown that the superconducting transition temperature in doped two-leg ladder cuprate superconductors increases with increasing pressure in the underpressure regime, and reaches a maximum in the optimal pressure, then decreases in the overpressure regime. This domed shape of the pressure dependence of the superconducting transition temperature is similar to that of the pressure dependence of the longitudinal part of the superconducting gap parameter, indicating that superconductivity in doped two-leg ladder cuprates is mainly produced by the development of the pairing correlation along legs.
Superconductivity in doped two-leg ladder cuprates
Within the t-J ladder model, superconductivity with a modified d-wave symmetry in doped two-leg ladder cuprates is investigated based on the kinetic energy driven superconducting mechanism. It is shown that the spin-liquid ground-state at the half-filling evolves into the superconducting ground-state upon doping. In analogy to the doping dependence of the superconducting transition temperature in the planar cuprate superconductors, the superconducting transition temperature in doped two-leg ladder cuprates increases with increasing doping in the underdoped regime, and reaches a maximum in the optimal doping, then decreases in the overdoped regime.
Low-energy excitations in 2-leg and 3-leg quantum spin ladders
Low-energy excitations in spin 1/2 antiferromagnetic Heisenberg spin ladders are studied by bosonization and gauge theoretical description. It is explicitly shown that zero modes in the bosonization play an essentially important role. Low-energy excitations in the 2-leg system is described by spin triplet Majorana fermions, and in the 3-leg case spin excitations on the top and bottom ladders are described by two massless scalar fields. It is predicted that if inter-ladder interaction between the top and bottom chains is introduced, a phase transition occures at some critical coupling and the system acquires an energy gap.
Charge density plateaux and insulating phases in the $t-J$ model with ladder geometry
We discuss the occurrence and the stability of charge density plateaux in ladder-like $t-J$ systems (at zero magnetization M=0) for the cases of 2- and 3-leg ladders. Starting from isolated rungs at zero leg coupling, we study the behaviour of plateaux-related phase transitions by means of first order perturbation theory and compare our results with Lanczos diagonalizations for $t-J$ ladders ($N=2\\times 8$) with increasing leg couplings. Furthermore we discuss the regimes of rung and leg couplings that should be favoured for the appearance of the charge density plateaux.
Magnetism in the pseudo-two-leg ladder compound CaCu{sub 2}O{sub 3}
Contrary to SrCu{sub 2}O{sub 3}, the pseudo-ladder two-leg compound CaCu{sub 2}O{sub 3} does not show a significant spin-gap behavior as expected for typical two-leg ladder systems. Magnetization measurements on single crystals show CaCu{sub 2}O{sub 3} to be a three-dimensional (3D) antiferromagnet (T {sub N}=27 K), showing relatively strong magnetic anisotropy and metamagnetic transitions. The 3D behavior is due to exchange between the ladders as deduced from electronic structure calculations within the local density approximation.
Results of measurements of gravitational sagging and torsions of the first prototype of long Si-ladder for the outer (the largest) layer of the Inner Tracker System (ITS) of ALICE are reported for different ladder orientations and different loads (up to 80g). 47.9 µm sagging in the middle of the ladder for the horizontal orientation of Si-wafers appear to be in a satisfactory agreement with the preliminary finite element calculations. The linearity of deformation as a function of load was also studied.
Hydropower dams on the Columbia River block migration of anadromous fishes. Extensive bypass facilities have been installed to guide migrating fishes around the dams. The first component of such systems encountered by out-migrating smolts is submerged bypass screens of one of two major design alternatives. Screens are constructed of either relatively fine woven mesh (traveling screens) or closely spaced bars (bar screens). Using underwater videoimaging, we quantified the relative fish impingement characteristics of each screen design at McNary Dam and The Dalles Dam. Each design was operated under a variety of deployment alternatives and turbine discharges. Video images of the screen surface were obtained from 5-6 cameras distributed on the screen centerline from the screen top (nearest the deck or intake) to the screen bottom. Over 4000 smolt-screen interactions were imaged, processed, and analyzed. A selection of hydraulic and behavioral variables was collected from each recorded image. Screen type had a significant effect on fish impingement and near-screen flow fields. These findings, in conjunction with the known influence of background noise levels on ability of fish to detect specific underwater signals, suggest that guidance effectiveness of a specific screen design may be partially determined by characteristics of the pressure field that the screen generates relative to background turbulence patterns - the Relative Pressure Signature Hypothesis.
Mechanical design, fabrication, and test of biomimetic fish robot using LIPCA as artificial muscle
This paper presents a mechanical design, fabrication and test of biomimetic fish robot using the Lightweight Piezocomposite Curved Actuator (LIPCA). We have designed a mechanism for converting actuation of the LIPCA into caudal fin movement. This linkage mechanism consists of rack-pinion system and four-bar linkage. We also have tested four types of caudal fin in order to examine effect of different shape of caudal fin on thrust generation by tail beat. Subsequently, based on the caudal fin test, four caudal fins which resemble fish caudal fin shapes of ostraciiform, subcarangiform, carangiform and thunniform, respectively, are attached to the posterior part of the robotic fish. The swimming test using 300 Vpp input with 1 Hz to 1.5 Hz frequency was conducted to investigate effect of changing tail beat frequency and shape of caudal fin on the swimming speed of the robotic fish. The maximum swimming speed was reached when the device was operated at its natural swimming frequency. At the natural swimming frequency 1 Hz, maximum swimming speeds of 1.632 cm/s, 1.776 cm/s, 1.612 cm/s and 1.51 cm/s were reached for ostraciiform-, subcarangiform-, carangiform- and thunniform-like caudal fins, respectively. Strouhal numbers, which are a measure of thrust efficiency, were calculated in order to examine thrust performance of the present biomimetic fish robot. We also approximated the net forward force of the robotic fish using momentum conservation principle.
Fish can sustain injury or mortality when they pass through hydroelectric facilities. To develop a method to monitor the passage and survival of juvenile salmonids without bias through turbines within the Federal Columbia River Power System, we developed and fabricated two designs of neutrally buoyant transmitters: Type A (sutured to the dorsal musculature of the fish anterior to the dorsal fin) and Type B (two-part design attached with wire pushed through the dorsal musculature, ventral to the dorsal fin). To determine the efficacy of the two designs under non-turbine passage-related conditions, fish had one of the tags attached and were held for 14 days to determine any potential effects of the tags on growth, survival and tissue damage. We also evaluated the attachment method by monitor...
Fluorescence in situ hybridization (FISH) is a useful method of determining the replication timing of specific genomic loci in mammals and of delineating replicon structures on DNA fibers in combination with in vivo replication labeling. In the case of simultaneous detection of a FISH probe and replicated forks, however, the DNA fibers are damaged by the DNA denaturation step for FISH detection, and the resulting fragmented fluorescence signals prevent analysis at high resolution. Here we found that hybridization of the probe to the genomic DNA was possible even under non-denaturing condition, but only at the time its genomic region replicated. Using the method designated non-denaturing FISH, we determined the replication timing of a specific BAC clone and the standard clones, and found that at least one replication origin exists within the genomic region covered by its BAC clone as an example.
Commercial fishery data from a proposed ocean thermal energy conversion (OTEC) site in Puerto Rico
This report describes the fish resources at a proposed OTEC site based upon commercial fisheries data from Puerto Rico. Records of commercial landings and relative fishing effort are used to examine temporal fluctuations in the abundance of exploited fish stocks at a proposed OTEC site located off the southeast coast of Puerto Rico (PROTEC), 17/sup 0/57'N, 65/sup 0/52'W). Such data are useful as a first approximation of natural fluctuations in the fish populations so that initial potential impacts of plant operations may be considered. The information will provide OTEC plant designers with an idea of the economic value of the fisheries in the vicinity of the proposed site. Detailed data are presented. (WHK)
The seaward migration of juvenile salmonids was monitored by the National Marine Fisheries Service (NMFS) at Bonneville and John Day Dams on the Columbia river in 1995. The NMFS Smolt Monitoring Project is part of a larger Smolt Monitoring Program (SMP) coordinated by the Fish Passage Center (FPC) for the Columbia Basin Fish and Wildlife Authority. This program focuses on protecting, mitigating, and enhancing fish populations affected by the development and operation of hydroelectric power plants on the Columbia River. The purpose of the SMP is to monitor the migration of the juvenile salmonid stocks in the Columbia basin and make flow and spill recommendations designed to facilitate fish passage. Data are also used for travel time, migration timing, and relative run size analysis. The purpose of the NMFS portion of the program is to provide FPC with species and project specific real time data from John Day and Bonneville Dams.
Risk assessment of parasitic helminths on cultured Nile tilapia (Oreochromis niloticus, L.)
Disease outbreaks constrain aquaculture development. Knowledge on the potential risks of pathogens to farmed fish can help in designing management strategies for increased aquaculture productivity and sustainability. This study compares the helminth infections in reservoirs and therein operated cages as well as earthen fish ponds and the feeding stream in order to assess the significance of life cycle style and water sources in parasite transmission. In addition field experiments were setup to determine loading time and transmission rate. From 650 fish examined, 8 helminth species were recorded (3 from caged- and all 8 from pond-raised fish). The parasite community was dominated by trophically-transmitted species in both culture systems indicating the importance of trophic pathway in helmi...
Optimization and Simulation in the Danish Fishing Industry
We consider the Danish fishing industry from a holistic viewpoint, and give a review of the main aspects, and the important actors. We also consider supply chain theory, and identify both theoretically, and based on other application areas, e.g. other fresh food industries, how optimization and simulation can be applied in a holistic modeling framework. Using the insights into supply chain theory and the Danish fishing industry, we investigate how the fishing industry as a whole may benefit from the formulation and use of mathematical optimization and simulation models. Finally, an appendix illustrates how a mathematical programming model may be designed to improve the planning of the fishing efforts for a group of fishermen.
Phagocytosis is a basic immunological function of mononuclear phagocytes and polymorphonuclear leukocytes. This process is a major defence mechanism in fish which involves recognition and killing of pathogenic microorganisms. It has been reported that phagocytic cells consume more oxygen and release several reactive oxygen species (ROS) during phagocytosis. This {open_quote}respiratory burst{close_quote} was first quantified by measuring the chemiluminescence (CL) emitted from human polymorphonuclear leukocytes and later in fish phagocytes. The oxygen intermediates responsible for this CL reaction include O{sub 2}{sup {minus}}, {center_dot}OH and H{sub 2}O{sub 2} which are also the major bactericidal agents in phagocytes{prime} oxygen-dependent killing process. Therefore, CL response can be used as an indicator of phagocytosis. This study is designed to examine the individual effects of mercury and selenium and also their possible interaction on CL response of fish pronephros phagocytes, because a defect in phagocytosis may predispose fish to diseases. 25 refs., 3 tabs.
This study was designed to investigate the effects of dietary lipid levels on growth performance, lipid deposition and activities of lipid metabolic enzymes in hybrid tilapia (Oreochromis niloticus × O. aureus). Four isonitrogenous (300 g/kg crude protein) experimental diets containing graded levels of lipid (25, 55, 85 and 115 g/kg) were randomly assigned to triplicate groups of 180 juvenile fish. Fish were fed twice daily for 8 weeks. At the end of the experiment, the growth performance and proximate composition of fish were determined. The activities and gene expression of lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) were assessed as well. Fish fed the diets with 55 and 85 g/kg lipid had a significantly (p tilapia, which is likely associated with the regulation of lipid metabolic enzymes including LPL and HSL. PMID:21114544
Summary This study was designed to investigate the effects of dietary lipid levels on growth performance, lipid deposition and activities of lipid metabolic enzymes in hybrid tilapia (Oreochromis niloticus--O. aureus). Four isonitrogenous (300-g/kg crude protein) experimental diets containing graded levels of lipid (25, 55, 85 and 115-g/kg) were randomly assigned to triplicate groups of 180 juvenile fish. Fish were fed twice daily for 8-weeks. At the end of the experiment, the growth performance and proximate composition of fish were determined. The activities and gene expression of lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) were assessed as well. Fish fed the diets with 55 and 85-g/kg lipid had a significantly (p--0.05) higher body weight gain than those fed the diets wit...
Fisheries and Oceans Canada - habitat management program in Ontario
On May 5, 2011, the Ontario Waterpower Association hosted the emergent hydro workshop in Peterborough. In the course of the workshop, Fisheries and Oceans Canada presented the habitat management program in Ontario. Fisheries and Oceans Canada explained that their role is to protect water resources. The Fisheries Act was passed to manage fisheries and fish habitats in Canada and to protect them from harmful alteration, disruption or destruction. The policy for the management of fish was written to interpret the Fisheries Act and enhance the productive capacity of fish habitats. In addition, two other Acts were passed, the Species at Risk Act and the Canadian Environmental Assessment Act, designed to protect species from extinction and improve coordination of, and public access to EA information. This presentation highlighted the different existing policies aimed at protecting fisheries and fish habitats in Canada.
Fish use of artificial dike structures in a navigable river
Abstract Artificial structures have been used to potentially increase fish production by providing cover, feeding and spawning areas. In some rivers, the United States Army Corps of Engineers has constructed dikes to provide additional habitat for river fishes and to mitigate for navigation impacts to young fishes. This study was designed to test whether such structures function as viable habitats as evidenced by increased abundances relative to reference areas lacking artificial dike structures. Dikes and reference sites in the Kanawha River, West Virginia were sampled 21 times between July 2002 and October 2003. We found a difference in taxonomic composition among fish using dikes relative to reference areas. Artificial dike structure use was most important among Centrarchidae species, e...
Summary This two-stage study was designed to determine the types and quantities of lost fishing gear and their distribution in the Gokova Special Environmental Protection Area (SEPA), eastern Mediterranean Sea. The study was carried out in approx. 15% of the total Gokova SEPA (7000 hectares), where traditional fishing is intensive. In the first phase of the study, the owners of all fishing boats (about 67% of the local fishermen in Gokova SEPA, n = 56) were interviewed using a structured questionnaire. In the second phase, diving surveys were conducted at 14 locations to locate and identify lost fishing gear, estimate the extent of the loss, and deduce the coherence between the diving survey and the questionnaire data. The questionnaire results showed that 0.84% of gillnets, 3.41% of tramm...
Diseases outbreaks are a major concern in intensive fish farming because fish are exposed to stressors which may negatively affect their physiology. This study set out to determine effects of dietary levamisole (Levamisole HCl; SIGMA) on performance and hematology of pacu, Piaractus mesopotamicus, juveniles. Fish (55.94 g) were stocked into 24 plastic aquaria (500 L; 15 fish per aquarium) and fed for 30 d with a commercial diet with 0, 50, 100, 200, 400, and 800 mg/kg levamisole, and for an extra 15 d, with a control diet in a totally randomized design trial (n = 4). Biometrical and hematological data were collected. No significant differences in growth parameters were recorded for either control or supplemented diets. Hematological parameters, such as hemoglobin, plasma glucose, white blo...
Araguas RM, Sanz N, Fernandez R, Utter FM, Pla C, Garcia-Marin J.-L. Genetic refuges for a self-sustained fishery: experience in wild brown trout populations in the eastern Pyrenees. Ecology of Freshwater Fish 2008: 17: 610-616. Copyright 2008 The Authors. Journal compilation Copyright 2008 Blackwell Munksgaard Abstract - Management policies balancing harvest and conservation of natural populations of fish are difficult to establish, both scientifically and politically. This issue is particularly difficult when those populations represent native genetic resources. Since 1997, several brown trout populations in the eastern Pyrenees Mountains (Spain) were designated as `genetic refuges' under varying fishing regulations, where releases of hatchery-origin fish are not permitted. We analysed g...
Numerous studies have been carried out to test the effect of incorporating various protein sources in salmonid fish feeds at the expense of fish meal. Comparison and interpretation of the results from these studies are difficult given the wide differences in ingredients, feed formulations, experimental designs and achieved performance. The objective of this study was to contrast two approaches for analyzing and comparing the results of such studies: meta-analysis and nutritional model simulation. The meta-analysis indicated that compared to fish meal-based diets, low or moderate dietary incorporation of plant protein ingredients in general did not affect growth of salmonids, whereas fish growth was depressed at high dietary inclusion levels of plant protein ingredients even when the diets ...
Commercial diets fed to coho salmon (Oncorhynchus kisutch) contain high levels of fish meal and fish oil. Given concerns about future supplies and prices of these commodities, more nutritional research aimed at identifying alternative dietary ingredients for Pacific salmon is needed. Thus, utilizing a factorial design, terrestrial proteins and lipids were evaluated in practical diets fed to coho salmon. The two basal diets, containing mostly marine proteins or containing only terrestrial proteins, were extruded and top-coated with either marine fish oil or a 63:37 mixture of canola and flaxseed oils. Each of the four dietary treatments was fed to quadruplicate groups of juvenile coho salmon initially weighing 7.5g/fish. At the end of the 15-wk feeding trial, weight gain (WG), feed efficien...
New single echo detection methods for shallow water fishery acoustics
Acoustic target detection is commonly carried out with parametric single echo detectors. These detectors test one ping at a time and look for echoes fulfilling a set of criteria such as echo duration and shape. In shallow water, noise phenomena can distort echoes from fish and false fish echoes can be generated. This causes the parametric detector to produce fractionated tracks from fish surrounded by numerous noise detections. Parametric detectors utilize only small portions of the information available in a split beam echogram. By including information from more than one ping and from the background reverberation, a more robust fish detector has been designed. This detector, called the Cross Filter Detector (CFD), has now been further improved by applying the variance in the angle measurements.
Fish oil affects immune function in 9 to 12 month old infants
Background - n-3 Polyunsaturated fatty acids (PUFA) are thought to affect immune function and may affect immune maturation in early life. Objective - To examine if fish oil supplementation in late infancy could modify immune function. Design - A 2×2 intervention with fish oil (3.4 ± 1.1 ml/day) or no fish oil and cow’s milk or infant formula from 9 to 12 month of age in 64 healthy Danish infants. Before and after the intervention we measured the fatty acid composition of erythrocyte (RBC) membranes, plasma IgE levels, C-reactive protein and soluble IL-2 receptors (sIL-2R) as well as cytokine production in whole-blood cultures stimulated with lipopolysaccharide (LPS)/phytohaemaglutinin (PHA) or Lactobacillus paracasei for 22 h. IgA was measured in feces at 10 months of age. Results - Fish oil supplementation effectively raised RBC n-3 PUFA (p
It is anticipated that as a result of the decision by Hydro-Quebec to create two new reservoirs on the upstream section of the Saint-Maurice River, the methylmercury (MeHg) content in the freshwater fishes inhabiting the river, will remain high for some time to come. This study is designed to assess the fish consumption rate and the likely increase in MeHg exposure of current fish consumers of this section of the river, namely recreational fisherman and the native population. Results of Monte Carlo simulations, based on average fish consumption rate of the target population, show exposure doses to MeHg from the Upper Saint-Maurice region of 145 ng/kg/day for sport fishermen and 92 ng/kg/day for the native population.
Acceptable sustainability in the fishing fleet
This article discusses the performance of the Norwegian fishing fleet within an acceptable level of sustainability. Previously, the cod-fishing fleet has been evaluated at the attributes-accident risk, employment, profitability, quality of the fish meat, catch capacity, greenhouse gas emissions/acidification, and bycatch/selection. The assessments focused on the first four steps of the systems engineering process, i.e., from needs identification to trade-offs of system alternatives. The objective of this paper is to focus on the last steps of the process; design, solve, verify, and test, to improve the decision-basis for fisheries management in order to increase sustainability in the fishing fleet. More specifically, this means to analyze the decision-making situation and develop acceptanc...
Viral hemorrhagic septicemia virus (VHSV) is an OIE-listed pathogen of fish, recently expanding in known host and geographic range in North America. Through a group process designed for subjective probability assessment, an international panel of fish health experts identified and weighted risk factors perceived important to the emergence and spread of the viral genotype, VHSV IVb, within and from the Great Lakes region of the US and Canada. Identified factors included the presence of known VHSV-susceptible species, water temperatures conducive for disease, hydrologic connectivity and proximity to known VHSV-positive areas, untested shipments of live or frozen fish from known positive regions, insufficient regulatory infrastructure for fish health oversight, and uncontrolled exposure to fo...
Swimming performance of the small characin Bryconamericus stramineus (Characiformes: Characidae)
Abstract in english Very little research has been conducted on the swimming capacity of Neotropical fish. The few studies available have focused on large migratory species. The present study used fixed and increasing velocity tests to determine prolonged and sustained speeds of the "pequira", Bryconamericus stramineus Eigenmann, 1908, a small, abundant species found in fish passages implemented at the Paraná basin, Brazil. The results of increasing velocity tests showed significant relation (more) ships between critical speeds, total and standard lengths, and body weight. When compared with other Neotropical fish, the "pequira" is able to swim faster than individuals of other species of similar length. The point of change from sustained to prolonged swimming was found to occur at an approximate speed of 8.7 lengths per second. These data provide guidance and criteria for design and proper maintenance of structures such as fishways, fish screens and other systems that aim to facilitate or avoid upstream passages as part of management strategies.
Anisakis spp. burden in Trachurus trachurus
Summary Anisakis is a parasite of marine mammals that uses a great number of fish species as intermediate or paratenic hosts. It is common in commercially important marine fishes and its presence is of great concern for both human health and economic reasons. Horse mackerels (Trachurus trachurus) originated from the Northern Aegean Sea were examined for the presence of Anisakis spp. larvae. The prevalence of Anisakis spp. was found 98.8 %. The number of parasites was significantly related to the host???s length but was not related to the fish gender. The month of sampling affected the size of the fishes and consequently the number of parasites. The length of larvae was not related to the host???s length. The present study resulted in the design of a prediction model for the number of exist...
Study of Fish Response Using Particle Image Velocimetry and High-Speed, High-Resolution Imaging
Existing literature of previous particle image velocimetry (PIV) studies of fish swimming has been reviewed. Historically, most of the studies focused on the performance evaluation of freely swimming fish. Technological advances over the last decade, especially the development of digital particle image velocimetry (DPIV) technique, make possible more accurate, quantitative descriptions of the flow patterns adjacent to the fish and in the wake behind the fins and tail, which are imperative to decode the mechanisms of drag reduction and propulsive efficiency. For flows generated by different organisms, the related scales and flow regimes vary significantly. For small Reynolds numbers, viscosity dominates; for very high Reynolds numbers, inertia dominates, and three-dimensional complexity occurs. The majority of previous investigations dealt with the lower end of Reynolds number range. The fish of our interest, such as rainbow trout and spring and fall chinook salmon, fall into the middle range, in which neither viscosity nor inertia is negligible, and three-dimensionality has yet to dominate. Feasibility tests have proven the applicability of PIV to flows around fish. These tests have shown unsteady vortex shedding in the wake, high vorticity region and high stress region, with the highest in the pectoral area. This evident supports the observations by Nietzel et al. (2000) and Deng et al. (2004) that the operculum are most vulnerable to damage from the turbulent shear flow, because they are easily pried open, and the large vorticity and shear stress can lift and tear off scales, rupture or dislodge eyes, and damage gills. In addition, the unsteady behavior of the vortex shedding in the wake implies that injury to fish by the instantaneous flow structures would likely be much higher than the injury level estimated using the average values of the dynamics parameters. Based on existing literature, our technological capability, and relevance and practicability to Department of Energy's Hydropower Program, we identified three major research areas of interest: free swimming, the boundary layer over fish, and kinematic response of fish. We propose that the highest priority is to characterize the kinematic response of fish to different turbulent environments such as high shear/turbulence and hydrodynamic disturbances created by solid structures such as deflector and turbine runner blade; the next priority is to map the boundary layer over swimming fish; the last is to document the behavior of freely swimming fish, focusing on fish of our interest. Grid turbulence and Karman vortex street will be employed to map the boundary layers over fish and investigate the effects of environmental disturbances on the swimming performance of fish, because they are well established and documented in engineering literature and are representative of fish's swimming environments. Extreme conditions characteristic of turbine environments, such as strong shear environment and collision, will be investigated. Through controlled laboratory studies, the fish injury mechanism from different sources will be evaluated in isolation. The major goals are to: gain first-hand knowledge of the biological effects under such extreme hydraulic environments in which fish could lack the capability to overcome the perturbations and be vulnerable to injury; Better understand field results by integrating the laboratory studies with the responses of sensor fish device; More importantly, provide well-defined validation cases and boundary conditions for geometry-based computational fluid-structure interaction modeling in order to simulate the complex hydraulic environments in advanced hydropower systems and their effects on fish, greatly enhancing the potential to use CFD as a bio-hydraulic design alternative.
Broadband antennas have various applications in digital terrestrial television (DTV) services. Compact broadband antennas are required for arranging in long and narrow space along the rim of a laptop display. A leaky-wave antenna using the composite right/left-handed transmission line (CRLH-TL) is one of the candidates for achieving the broadband antenna. However, there are not enough to design guideline of small leaky wave antennas using the CRLH-TL for UHF band. In this paper, a CRLH-TL comprising a ladder network is proposed for broadband and simple structure. The paper also discusses the design of a leaky-wave antenna with the CRLH-TL operating in the DTV band. The relation between the operating bandwidth and attenuation constant of the CRLH-TL is discussed. An antenna that can be accommodated in the limited and narrow space available in mobile terminals has to be designed. Hence, the effects of the number of cells and a finite ground plane are discussed with the purpose of achieving the miniaturization of the antenna. In this study, the transmission and radiation characteristics of the fabricated antennas are measured. The gain of the fabricated antenna is confirmed to remain almost constant even when the operating frequency is varied. The maximum gain and operating band achieved in this study are approximately ?0.6dBi and about 54%, respectively.
Powering via Cooling Pipes: an Optimized Design for an SLHC Silicon Tracker
Silicon trackers at the SLHC will suffer high radiation damage from particles produced during the collisions, which leads to high leakage currents. Reducing these currents in the sensors requires efficient cooling to -30 C. The large heat of evaporation of CO2 and the low viscosity allows for a two-phase cooling system with thin and long cooling pipes, because the small flow of liquid needed leads to negligible temperature drops. In order to reduce the material budget a system is proposed in which a large scale tracker requiring ca. 50 kW of power is powered via 1-2 mm diameter aluminum cooling pipes with a length of several m. These long cooling pipes allow to have all service connections outside the tracking volume, thus reducing the material budget significantly. The whole system is designed to have negligible thermal stresses. A CO2 blow system has been designed and first tests show the feasibility of a barrel detector with long ladders and disks at small radii leading to an optimized design with respect ...
Construction and evaluation of salmonid habitat improvements on Fish Creek, a tributary of the upper Clackamas River, was continued in fiscal year 1986 by the Estacada Ranger District, Mt. Hood National Forest, and the Anadromous Fish Habitat Research Unit of the Pacific Northwest Research Station (PNW), USDA Forest Service. The study began in 1982 when PNW entered into an agreement with the Mt. Hood National Forest to evaluate fish habitat improvements in the Fish Creek basin on the Estacada Ranger District. The project was initially conceived as a 5-year effort (1982-1986) to be financed with Forest Service funds. The habitat improvement program and the evaluation of improvements were both expanded in mid-1983 when the Bonneville Power Administration (BPA) entered into an agreement with the Mt. Hood National Forest to cooperatively fund work on Fish Creek. Habitat improvement work in the basin is guided by the Fish Creek Habitat Rehabilitation-Enhancement Framework developed cooperatively by the Estacada Ranger District, the Oregon Department of Fish and Wildlife, and the Pacific Northwest Research Station (see Appendix 2). The framework examines potential factors limiting production of salmonids in the basin, and the appropriate habitat improvement measures needed to address the limiting factors. Habitat improvement work in the basin has been designed to: (1) improve quantity, quality, and distribution of spawning habitat for coho and spring chinook salmon and steelhead trout, (2) increase low flow rearing habitat for steelhead trout and coho salmon, (3) improve overwintering habitat for coho salmon and steelhead trout, (4) rehabilitate riparian vegetation to improve stream shading to benefit all species, and (5) evaluate improvement projects from a drainage wide perspective. The objectives of the evaluation include: (1) Drainage-wide evaluation and quantification of changes in salmonid spawning and rearing habitat resulting from a variety of habitat improvements. (2) Evaluation and quantification of changes in fish populations and biomass resulting from habitat improvements. (3) Benefit-cost analysis of habitat improvements developed with BPA and Forest Service funds on Fish Creek.
Natural reproduction in the Kootenai River white sturgeon population has not produced a successful year class since 1974, resulting in a declining broodstock and 20 consecutive year classes missing from the age-class structure. This report describes a captive breeding plan designed to preserve the remaining genetic variability and to begin rebuilding the natural age class structure. The captive breeding program will use 3--9 females and an equal number of males captured from the Kootenai River each spring. Fish will be spawned in pairs or in diallel mating designs to produce individual families that will be reared separately to maintain family identity. Fish will be marked to identify family and year class before return to the river. Fish should be returned to the river as fall fingerlings to minimize potential adaptation to the hatchery environment Initially, while tagging methods are tested to ensure positive identification after return to the river, it may be necessary to plant fish as spring yearlings. Number of fish planted will be equalized at 5,000 per family if fall fingerlings or 1,000 per family if spring yearlings. Assuming annual survival rates of 20% during the first winter for fall fingerling plants and 50% for years 1--3, and 85% for years 4--20 of all fish planted, the target numbers would yield 7.9 progeny per family or about 4 breeding pairs at age 20. Natural survival in the river environment during the 19+ years from planting to maturity would result in variability in genetic contribution of families to the next broodstock generation. Fish planted per family would be adjusted in future years when actual survival rate information is known. Broodfish will be tagged when captured to minimize multiple spawning of the same fish. implementation of this breeding plan each year for the 20-year generation interval, using 5 different mating pairs each year, will yield an effective population size of 200, or 22.5% of the estimated 1990 population.
A new highly abundant satellite DNA from Discoglossus pictus (Dp-sat1) was isolated and characterized. The repetitive unit (0.51 kb) has 2 HindIII sites and only one SpeI site: digestion of genomic DNA with HindIII produces 3 fragments: HA (0.17 kb), HB (0.34 kb), and HC = HA + HB (0.51 kb), while digestion with SpeI produces the whole repetitive unit (0.51 kb) that contains both HindIII sites. Sequence analysis of cloned repeats indicates an average A + T content of 71%, with many A- and T-runs. Southern blot analysis shows an arrangement of multiple bands of the 0.51 kb monomer in SpeI-digested DNA, while HindIII-digested DNA shows a ladder composed of all the possible combinations of the 3 digested fragments. Quantitative dot-blot indicates that Dp-sat1 accounts for about 6% of the D. pictus genome: this value represents about 1.5 x 10(6) copies of repetitive units per nucleus. This satellite DNA is also a major repetitive DNA in 4 other Discoglossus species, in which the repetitive unit presents the same size and restriction sites except in D. montalentii where it contains a unique HindIII site. This satellite DNA was absent in all the other tested archaeo- and neo-bratrachian species, as well as non-amphibian species. Fluorescent in situ hybridization (FISH) analysis shows that Dp-sat1 is localized only in peri- and/or para-centromeric areas of the 7 small chromosome pairs, while no labeling was observed in the 7 large chromosome pairs. Remarkably, Dp-sat1 heterochromatin is found only at one pole of the nucleus, suggesting that during interphase all 7 small chromosome pairs are located in the same nuclear region. PMID:20110657
In the advent of the installation of a PIT-tag interrogation system in the Cascades Island fish ladder at Bonneville Dam (BON), and other CRB dams, this overview describes in general terms what can and cannot be estimated under seven different scenarios of adult PIT-tag detection capabilities in the CRB. Moreover, this overview attempted to identify minimal adult PIT-tag detection configurations required by the ten threatened Columbia River Basin (CRB) chinook and steelhead ESUs. A minimal adult PIT-tag detection configuration will require the installation of adult PIT-tag detection facilities at Bonneville Dam and another dam above BON. Thus, the Snake River spring/summer and fall chinook salmon, and the Snake River steelhead will require a minimum of three dams with adult PIT-tag detection capabilities to guarantee estimates of ''ocean survival'' and at least of one independent, in-river returning adult survival (e.g., adult PIT-tag detection facilities at BON and LGR dams and at any other intermediary dam such as IHR). The Upper Columbia River spring chinook salmon and steelhead will also require a minimum of three dams with adult PIT-tag detection capabilities: BON and two other dams on the BON-WEL reach. The current CRB dam system configuration and BPA's and COE's commitment to install adult PIT-tag detectors only in major CRB projects will not allow the estimation of an ''ocean survival'' and of any in-river adult survival for the Lower Columbia River chinook salmon and steelhead. The Middle Columbia River steelhead ESU will require a minimum of two dams with adult PIT-tag detection capabilities: BON and another upstream dam on the BON-McN reach. Finally, in spite of their importance in terms of releases, PIT-tag survival studies for the Upper Willamette chinook and Upper Willamette steelhead ESUs cannot be perform with the current CRB dam system configuration and PIT-tag detection capabilities.
BIOTIC INTEGRITY OF STREAMS IN THE SAVANNAH RIVER SITE INTEGRATOR OPERABLE UNITS, 1996 TO 2003
The Savannah River Site (SRS) has been divided into six Integrator Operable Units (IOUs) that correspond to the watersheds of the five major streams on the SRS (Upper Three Runs, Fourmile Branch, Pen Branch, Steel Creek, and Lower Three Runs) and the portions of the Savannah River and Savannah River Swamp associated with the SRS. The streams are the primary integrators within each IOU because they potentially receive, through surface or subsurface drainage, soluble contaminants from all waste sites within their watersheds. If these contaminants reach biologically significant levels, they would be expected to effect the numbers, types, and health of stream organisms. In this study, biological sampling was conducted within each IOU as a measure of the cumulative ecological effects of the waste sites within the IOUs. The use of information from biological sampling to assess environmental quality is often termed bioassessment. The IOU bioassessment program included 38 sites in SRS streams and nine sites in the Savannah River. Sampling was conducted in 1996 to 1998, 2000, and 2003. Four bioassessment methods were used to evaluate ecological conditions in the IOU streams: the Index of Biotic Integrity, the Fish Health Assessment Index, measurement of fish tissue contaminant levels, and two benthic macroinvertebrate indices. The Index of Biotic Integrity (IBI) is an EPA supported method based on comparison of ecologically important and sensitive fish assemblage variables between potentially disturbed and reference (i.e., undisturbed) sites. It is designed to assess the ability of a stream to support a self-sustaining biological community and ecological processes typical of undisturbed, natural conditions. Since many types of contaminants can bioaccumulate, fish tissue contaminant data were used to determine the types of chemicals fish were exposed to and their relative magnitudes among IOUs. The Fish Health Assessment Index (HAI) is an EPA supported method for assessing the health and condition of individual fish based on dissection and internal examination. It helped to determine whether contaminant concentrations were high enough to adversely affect the health of individual fish. The benthic macroinvertebrate multimetric index (HDMI), used in 1997 to 2000, is a method for assessing stream health based on macroinvertebrate data collected with Hester-Dendy artificial substrates. In 2003 it was replaced with the Multiple Habitat Sampling protocol, a SCDHEC method for collecting and analyzing benthic macroinvertebrate data from natural substrate. These two macroinvertebrate based methods were used in conjunction with the fish based IBI to provide a more comprehensive assessment of ecological conditions. Lastly, habitat data were collected from each stream to assist in determining whether ecological integrity was compromised by physical factors (e.g., erosion) or chemical factors (e.g., discharge of toxic materials). Fish from many SRS streams exhibited evidence of contamination as a result of current or former SRS operations. The most prevalent radiological contaminants were cesium-137 (highest in fish from Lower Three Runs followed by Steel Creek and Fourmile Branch), tritium (highest in fish from Fourmile Branch followed by Pen Branch, and the Savannah River swamp), and strontium (highest in fish from Fourmile Branch followed by Pen Branch). Radiological contaminants were also found in fish collected from the Savannah River near the mouths of contaminated SRS streams; however, contaminant levels were substantially lower than in fish from the streams themselves. Mercury levels were moderately elevated in fish from some streams, particularly Lower Three Runs, and in fish from the Savannah River. Despite the occurrence of contaminants, most SRS streams exhibited comparatively high biotic integrity (based on IBI, HDMI, and MHSP scores) and minimal levels of pathology among individual fish (e.g., presence of tumors or extreme thinness), indicating that contaminant levels were generally insufficient to cause significant ecological degradation.
Superexchange in the quarter- filled two- leg ladder system NaV2O5
A theory of superexchange in the mixed valent layer compound NaV2O5 is presented which provides a consistent description of exchange both in the disordered and charge ordered state. Starting from results of band structure calculations for NaV2O5 first an underlying electronic model for a ladder unit in the Trellis lattice is formulated. By using the molecular orbital representation for intra-rung electronic states a second-order perturbation procedure is developed and an effective spin-chain model for a ladder is derived. Variation of the resulting superexchange integral J is examined numerically as the ladder system evolves from a charge disordered to the extreme ('zig-zag') charge ordered state. It is found that the effective intra- ladder superexchange is always antiferromagnetic.
Effective Charge and Spin Hamiltonian for the Quarter-Filled Ladder Compound $\\alpha'-NaV_{2}O_{5}$
An effective intra- and inter-ladder charge-spin hamiltonian for the quarter-filled ladder compound $\\alpha'$-NaV$_2$O$_5$ has been derived by using the standard canonical transformation method. In the derivation, it is clear that a finite inter-site Coulomb repulsion is needed to get a meaningful result otherwise the perturbation becomes ill-defined. Various limiting cases depending on the values of the model parameters have been analyzed in detail and the effective exchange couplings are estimated. We find that the effective intra-ladder exchange may become ferromagnetic for the case of zig-zag charge ordering in a purely electronic model. We estimate the magnitude of the effective inter-rung Coulomb repulsion in a ladder and find it to be about one-order of magnitude too small in order to stabilize charge-ordering.
Spin-liquid versus dimerized ground states in a frustrated Heisenberg antiferromagnet
We present a Density Matrix Renormalization Group (DMRG) study of the ground-state properties of spin-1/2 frustrated $J_1-J_3$ Heisenberg $n_l$-leg ladders (with $n_l$ up to 8). For strong frustration ($J_3/J_1\\simeq 0.5$), both even- and odd-leg ladders display a finite gap to spin excitations, which we argue remains finite in the two-dimensional limit. In this regime, on odd-leg ladders the ground state is spontaneously dimerized, in agreement with the Lieb-Schultz-Mattis prediction, while on even-leg ladders the dimer correlations decay exponentially. The magnitude of the dimer order parameter decreases as the number of legs increases, consistent with a two-dimensional spin-liquid ground state.
Determining the structure of the superconducting gap in $Cu_{2}O_{3}$ 2-leg ladder materials
Superconductivity has been recently observed in Sr_{0.4}Ca_{13.6}Cu_{24}O_{41.84} which contains quasi-one-dimensional Cu_{2}O_{3} 2-leg ladders. If, as suggested by some theories, the superconductivity arises from these 2-leg ladders, it will be important to determine the structure of the superconducting gap. In particular, does the gap in a 2-leg ladder change sign when one goes from the bonding to antibonding fermi surface points? Here we carry out phenomenological calculations of nuclear relaxation rates and inelastic neutron scattering intensity in order to provide estimates of the experimental resolution that will be required to determine the structure of the superconducting gap associated with an array of weakly coupled 2-leg ladders.
The transition between hole-pairs and four-hole clusters in four-leg tJ ladders
Holes weakly doped into a four-leg \\tj ladder bind in pairs. At dopings exceeding a critical doping of $\\delta_c\\simeq {1/8}$ four hole clusters are observed to form in DMRG calculations. The symmetry of the ground state wavefunction does not change and we are able to reproduce this behavior qualitatively with an effective bosonic model in which the four-leg ladder is represented as two coupled two-leg ladders and hole-pairs are mapped on hard core bosons moving along and between these ladders. At lower dopings, $\\delta<\\delta_c$, a one dimensional bosonic representation for hole-pairs works and allows us to calculate accurately the Luttinger liquid parameter $\\krho$, which takes the universal value $\\krho=1$ as half-filling is approached.
Magnetization Curves of Antiferromagnetic Heisenberg Spin-1/2 Ladders
Magnetization processes of spin-1/2 Heisenberg ladders are studied using strong-coupling expansions, numerical diagonalization of finite systems and a bosonization approach. We find that the magnetization exhibits plateaux as a function of the applied field at certain rational fractions of the saturation value. Our main focus are ladders with 3 legs where plateaux with magnetization one third of the saturation value are shown to exist.
Alkyl(chloro)ethoxygermanes were hydrolyzed with water to give 1,3,5-trialkyl-1,3,5-trichlorocyclotrigermoxanes. Hydrolysis of 1,3,5-trichlorocyclotrigermoxanes gave 5,7-dichloro-1,3,5,7,9,11-hexaalkyltricyclo[7.3.1.13,7]hexagermoxanes. The tricylic anti-form ladder hexagermoxanes reacted with water to afford hexakis(alkylgermasesquioxane)s. These cage and ladder germoxanes were identified by spectrospcopic and X-ray diffraction methods.
We recall results concerning one-dimensional classical and quantum systems with ladder operators. We obtain the most general one-dimensional classical systems respectively with a third and a fourth order ladder operators satisfying polynomial Heisenberg algebras. These systems are written in terms of the solutions of quartic and quintic equations. We use these results to present two new families of superintegrable systems and examples of trajectories that are deformed Lissajous's figures.
Synthesis of Polydiacetylene-Based Ladder Polymer
A novel polydiacetylene (PDA) was prepared from the monomer having two butadiyne parts linked by a chain of methylene units. Polymerization is solid state was performed by UV or ?-ray irradiation. From 13C NMR spectra measured using CP/MAS technique, the structure of the ladder polymer composed of two PDAs linked by methylene chains was confirmed. By X-ray diffraction experiment, crystallinity was found to be preserved even for the ladder polymer.
Response to Spin-Singlet clusters in the ladder compound NaV2O5
This response on a Comment on Reference 2 discusses the space group Fmm2 determination of the low temperature structure of NaV2O5. We show that while conventional crystallography is consistent with 'zig-zag' charge ordering of half of the ladders, recent spectroscopic measurements suggest that the symmetry is lower than Fmm2. We suggest space group C2, consistent with full 'zig-zag' charge ordering of all the ladders.
The massless supersymmetric ladder with L rungs
We show that in the massless Formula Not Shown supersymmetric Wess-Zumino theory it is possible to devise a computational strategy by which the x-space calculation of the ladder 4-point correlators can be carried out without introducing any regularization. As an application we derive a representation valid at all loop orders in terms of conformal invariant integrals. We obtain an explicit expression of the 3-loop ladder diagram for collinear external points.
The massless supersymmetric ladder with L rungs
We show that in the massless N=1 supersymmetric Wess-Zumino theory it is possible to devise a computational strategy by which the x-space calculation of the ladder 4-point correlators can be carried out without introducing any regularization. As an application we derive a representation valid at all loop orders in terms of conformal invariant integrals. We obtain an explicit expression of the 3-loop ladder diagram for collinear external points.
Re-Entrant Quantum Phase Transitions in Antiferromagnetic Spin-1 Ladders
In response to recent attempts to construct higher-spin ladder materials from organic polyradicals, we study the ground-state properties of a wide class of antiferromagnetic spin-1 ladders. Employing various numerical tools, we reveal the rich phase diagram and correct a previous nonlinear-sigma-model prediction. A variational analysis interprets the phase competition well with particular emphasis on the re-entrant phase boundary as a function of the rung interaction.
Resonant magnetic mode in superconducting 2-leg ladders
The spin dynamics of a doped 2-leg spin ladder is investigated by numerical techniques. We show that a hole pair-magnon boundstate evolves at finite hole doping into a sharp magnetic excitation below the two-particle continuum. This is supported by a field theory argument based on a SO(6)-symmetric ladder. Similarities and differences with the resonant mode of the high-T$_c$ cuprates are discussed.
Phase diagrams of spin ladders with ferromagnetic legs
The low-temperature properties of the spin S=1/2 ladder with anisotropic ferromagnetic legs are studied using the continuum limit bosonization approach. The weak-coupling ground state phase diagram of the model is obtained for a wide range of coupling constants and several unconventional gapless ''spin-liquid'' phases are shown to exist for ferromagnetic coupling. The behavior of the ladder system in the vicinity of the ferromagnetic instability point is discussed in detail.
Stripes on a 6-Leg Hubbard Ladder
While DMRG calculations find stripes on doped n-leg t-J ladders, little is known about the possible formation of stripes on n-leg Hubbard ladders. Here we report results for a 7x6 Hubbard model with 4 holes. We find that a stripe forms for values of U/t ranging from 6 to 20. For U/t ~ 3-4, the system exhibits the domain wall feature of a stripe, but the hole density is very broadened.
An Algebraic q-Deformed Form for Shape-Invariant Systems
A quantum deformed theory applicable to all shape-invariant bound-state systems is introduced by defining q-deformed ladder operators. We show these new ladder operators satisfy new q-deformed commutation relations. In this context we construct an alternative q-deformed model that preserve the shape-invariance property presented by primary system. q-deformed generalizations of Morse, Scarf, and Coulomb potentials are given as examples.
Origin of the second coherent peak in the dynamical structure factor of a warped spin-ladder
Appearance of the second coherent peak in the dynamical structure factor of a warped (non-symmetric) spin ladder is suggested. The general arguments are confirmed by the first order (with respect to warping) calculation for a spin ladder with singlet-rung ground state. Basing on this result a new interpretation is proposed for the inelastic neutron scattering data in the spin gap compound CuHpCl.
Spiroligozymes for transesterifications: design and relationship of structure to activity.
Transesterification catalysts based on stereochemically defined, modular, functionalized ladder-molecules (named spiroligozymes) were designed, using the "inside-out" design strategy, and mutated synthetically to improve catalysis. A series of stereochemically and regiochemically diverse bifunctional spiroligozymes were first synthesized to identify the best arrangement of a pyridine as a general base catalyst and an alcohol nucleophile to accelerate attack on vinyl trifluoroacetate as an electrophile. The best bifunctional spiroligozyme reacted with vinyl trifluoroacetate to form an acyl-spiroligozyme conjugate 2.7 × 10(3)-fold faster than the background reaction with a benzyl alcohol. Two trifunctional spiroligozymes were then synthesized that combined a urea with the pyridine and alcohol to act as an oxyanion hole and activate the bound acyl-spiroligozyme intermediate to enable acyl-transfer to methanol. The best trifunctional spiroligozyme carries out multiple turnovers and acts as a transesterification catalyst with k(1)/k(uncat) of 2.2 × 10(3) and k(2)/k(uncat) of 1.3 × 10(2). Quantum mechanical calculations identified the four transition states of the catalytic cycle and provided a detailed view of every stage of the transesterification reaction. PMID:22992057
The motional Stark effect (MSE) diagnostic is routinely used to determine the q-profile in large fusion devices. To apply the MSE diagnostic to experiments with low magnetic fields such as NSTX (<1 T), a tunable birefringent Lyot filter is used with high throughput and high resolution which allows for a good signal-to-noise ratio. The birefringent filter is made from lithium-niobate crystals, which are coated with a layer of indium tin-oxide (ITO). The ITO layer is a transparent conductive coating. By applying an electric field across the crystal the index of refraction is varied. This allows tunability of the filter. Putting multiple crystals together and tuning them individually it is possible to pass certain wavelengths of light and reject others. A high voltage ramp generator circuit is under development to ramp a 5 kV signal using a simple design involving MOSFET ladders. The goal is to design the circuit so that it can ramp ±5000 volts at a frequency of around 1 kHz. This would allow the filter to sweep over a range of ˜ 1nm.
As the homelessness ‘crisis’ in the United States enters a third decade, few are as adversely affected as persons with serious mental illness. Despite recent evidence favoring a ‘housing first’ approach, the dominant ‘treatment first’ approach persists in which individuals must climb a ladder of program requirements before becoming eligible for an apartment of their own. Drawing upon the concept of ‘ontological security’, this qualitative study examines the subjective meaning of ‘home’ among 39 persons who were part of a unique urban experiment that provided New York City’s homeless mentally ill adults with immediate access to independent housing in the late 1990s. The study design involved purposively sampling from the experimental (housing first) group (N=21) and the control (treatment first) group (N=18) and conducting two life history interviews with each participant. Markers of ontological security--constancy, daily routines, privacy, and having a secure base for identity construction—provided sensitizing concepts for grounded theory analyses designed to also yield emergent, or new, themes. Findings revealed clear evidence of the markers of ontological security among participants living in their own apartments. This study expands upon previous research showing that homeless mentally ill persons are capable of independent living in the community. The emergent theme of ‘what’s next’ questions and uncertainty about the future points to the need to address problems of stigma and social exclusion that extend beyond the minimal achievement of having a ‘home’.
As the homelessness 'crisis' in the United States enters a third decade, few are as adversely affected as persons with serious mental illness. Despite recent evidence favoring a 'housing first' approach, the dominant 'treatment first' approach persists in which individuals must climb a ladder of program requirements before becoming eligible for an apartment of their own. Drawing upon the concept of 'ontological security', this qualitative study examines the subjective meaning of 'home' among 39 persons who were part of a unique urban experiment that provided New York City's homeless mentally ill adults with immediate access to independent housing in the late 1990s. The study design involved purposively sampling from the experimental (housing first) group (N=21) and the control (treatment first) group (N=18) and conducting two life history interviews with each participant. Markers of ontological security-constancy, daily routines, privacy, and having a secure base for identity construction-provided sensitizing concepts for grounded theory analyses designed to also yield emergent, or new, themes. Findings revealed clear evidence of the markers of ontological security among participants living in their own apartments. This study expands upon previous research showing that homeless mentally ill persons are capable of independent living in the community. The emergent theme of 'what's next' questions and uncertainty about the future points to the need to address problems of stigma and social exclusion that extend beyond the minimal achievement of having a 'home'. PMID:17355900
Energy for the future. New solutions - made in Germany
Today we are once again in the middle of a new industrial and energy technology revolution. From a technology point of view, it is even a huge positive, as it opens up new markets for new and more energy and natural efficient solutions. Under this aspect, the paper under considerations consists of the following contributions: (a) From grassroots movement to political power; (b) Constructive experimentation; (c) Degrees for a green future (German universities offer a wide variety of courses in renewable energy); (d) Climbing the green career ladder (Diverse career opportunities in the renewable energy sector); (e) Natural power plants: Energy you can count on (German researchers successfully focus on the sun's energy); (f) Concentrated energy from the ocean (Dynamic development of wind energy in Germany); (g) Powerful waves and extraordinary treasures (German water experts are in demand all over the world); (h) Designer diesel and deep heat (Germany leads the fields in biofuels); (i) Sending the right signals (Climate protection as an opportunity for change); (k) Car today, bike tomorrow (Environmental psychologist Ellen Matthies); (l) The secret lies under the Bonnet (Hybrid technology paves the way for ''clean'' buses and trains); (m) Pioneering the ''silent'' car (Researchers put their foot on the accelerator for electromobility); (n) The school of the future (Students at RWTH Aachen University design an energy project for the classroom).
Passage and survival data for yearling and subyearling Chinook salmon and juvenile steelhead were collected at McNary Dam between 2006 and 2009. These data have provided critical information for resource managers to implement structural and operational changes designed to improve the survival of juvenile salmonids as they migrate past the dam. Much of the information collected at McNary Dam was in the form of three-dimensional tracks of fish movements in the forebay. These data depicted the behavior of multiple species (in three dimensions) during different diel periods, spill conditions, powerhouse operations, and test configurations of the surface bypass structures (temporary spillway weirs; TSWs). One of the challenges in reporting three-dimensional results is presenting the information in a manner that allows interested parties to summarize the behavior of many fish over many different conditions across multiple years. To accomplish this, we investigated the feasibility of using a Markov chain analysis to characterize fish movement patterns in the forebay of McNary Dam. The Markov chain analysis is one way that can be used to summarize numerically the behavior of fish in the forebay. Numerically summarizing the behavior of juvenile salmonids in the forebay of McNary Dam using the Markov chain analysis allowed us to confirm what had been previously summarized using visualization software. For example, proportions of yearling and subyearling Chinook salmon passing the three powerhouse areas was often greater in the southern and middle areas, compared to the northern area. The opposite generally was observed for steelhead. Results of this analysis also allowed us to confirm and quantify the extent of milling behavior that had been observed for steelhead. For fish that were first detected in the powerhouse region, less than 0.10 of the steelhead, on average, passed within each of the powerhouse areas. Instead, steelhead transitioned to adjoining areas in the spillway before passing the dam. In comparison, greater than 0.20 of the Chinook salmon passed within the powerhouse areas. Less milling behavior was observed for all species for fish that first approached the spillway. Compared to the powerhouse areas, a higher proportion of fish, regardless of species, passed the spillway areas and fewer transitioned to adjoining areas in the powerhouse. In addition to quantifying what had been previously speculated about the behavior of fish in the forebay of McNary Dam, the Markov chain analysis refined our understanding of how fish behavior and passage can be influenced by changes to the operations and structure of McNary Dam. For example, the addition of TSWs to the spillway area clearly influenced the passage of fish. Previous results have been reported showing that TSWs increased the number of fish passing through non-turbine routes and the fish-track videos indicated, in general, how fish behaved before passing through the TSWs. However, the analysis presented in this report allowed us to better understand how fish moved across the face of the dam before passing the TSWs and provided a way to quantify the effect of TSW location. Installation of the TSWs in bays 22 and 20 clearly increased passage proportions through the southern one-third of the spillway area for all species, most significantly for steelhead. When the TSWs were moved to bays 19 and 20 in 2008, overall passage through the southern one-third of the spillway remained higher than 2006, but decreased from what was observed in 2007. Shifting the TSWs to the north decreased the proportion of fish passing through the TSWs and increased the number of fish that moved to adjoining areas before passing the dam. Perhaps the most interesting new information to come out of the two-step Markov chain analysis relates to how the performance of the TSWs was influenced by their proximity to the powerhouse. During 2007, the highest proportion of fish passing through TSW22 was for fish that transitioned from the powerhouse area. In contrast, a relatively low proportion of fish passed through TSW20 after coming from the powerhouse area. Instead, the proportion of fish that passed TSW20 after coming from the northern part of the spillway was twice as high as the proportion of fish that passed through TSW20 after coming from the powerhouse. During 2008, the TSW in bay 22 was moved to bay 19, leaving the TSW in bay 20 as the one closest to the powerhouse. As was the case when a TSW was located in bay 22; the proportion of fish passing TSW20 after coming from the powerhouse was greater than the proportion of fish passing through TSW20 after coming from the northern part of the spillway. Passage proportions for fish passing through TSW19, the farthest north of the two TSWs during 2008, was higher for fish that came from the northern part of the spillway compared to the proportion of fish that passed through TSW19 after coming from the powerhouse. The Markov chain analysis provided a mathematical way to characterize fish behavior in the forebay of McNary Dam and helped refine our understanding of how fish movements were influenced by operational and structural changes at McNary Dam. The Markov chain analysis also could be used to examine how future structural and operational changes proposed for McNary Dam might influence the passage of juvenile salmonids.
Food safety management in the fish industry in Mauritius: knowledge, attitude and perception
Purpose - The objective of this study, part of a larger overall project on food safety management in the fish producing and processing companies in Mauritius, is to generate information on the knowledge, attitude and perception of key informants at the managerial level in these sectors with respect to food safety and its management. Design/methodology/approach - A structured questionnaire was designed to evaluate the knowledge and understand the attitude and perception of the key informants. Personal interviews were carried out on-board four fishing vessels and in seven fish processing establishments. Findings - The main reason provided by all companies to have a documented food safety management system was to comply with the importing countries' legislative requirements. Interviewees expr...
Use of Fully Biodegradable Panels to Reduce Derelict Pot Threats to Marine Fauna
Abstract Fishing pots (i.e., traps) are designed to catch fish or crustaceans and are used globally. Lost pots are a concern for a variety of fisheries, and there are reports that 10-70% of deployed pots are lost annually. Derelict fishing pots can be a source of mortality for target and bycatch species for several years. Because continual removal of derelict gear can be impractical over large spatial extents, modifications are needed to disarm gear once it is lost. We tested a fully biodegradable panel with a cull or escape ring designed for placement on the sides of a crab pot that completely degrades into environmentally neutral constituents after approximately 1 year. This panel is relatively inexpensive, easy to install, and can be used in multiple fisheries. We used the blue crab (Ca...
Research conducted as part of the Comprehensive Cooling Water Study (CCWS) has elucidated many factors that are important to fish population and community dynamics in a variety of habitats on the Savannah River Plant (SRP). Information gained from these studies is useful in predicting fish responses to SRP operations. The overall objective of the CCWS was (1) to determine the environmental effects of SRP cooling water withdrawals and discharges and (2) to determine the significance of the cooling water impacts on the environment. The purpose of this study was to: (1) examine the effects of thermal plumes on anadromous and resident fishes, including overwintering effects, in the SRP swamp and associated tributary streams; (2) assess fish spawning and locate nursery grounds on the SRP; (3) examine the level of use of the SRP by spawning fish from the Savannah River, this objective was shared with the Savannah River Laboratory, E.I. du Pont de Nemours and Company; and (4) determine impacts of cooling-water discharges on fish population and community attributes. Five studies were designed to address the above topics. The specific objectives and a summary of the findings of each study are presented.
Response of mosquitofish (Gambusia affinis) populations to seasonally unpredictable perturbations
Many questions remain unresolved about the linkages between life history attributes of fishes and the tactics that these organisms employ in response to environmental uncertainty. Such questions include (1). If a perturbation affects the entire ecosystem, what are the consequences for a given population of fish (2) What tactics can a fish employ to increase its chances of leaving offspring (3) Do fish respond differently to such perturbations depending on the season (4) How do these changes relate to the overall resilience of the population The research reported here was designed to address such questions. Mosquito fish (Gambusia affinis) populations in thirteen experimental ponds at Oak Ridge National Laboratory were sampled ten times between June 1988, and July 1989 in response to a series of chemical disturbances. During each sampling period the population size and total biomass of Gambusia in each pond was estimated using photographs and a length weight regression. Size-frequency histograms were used to examine seasonal and dose-related changes in population structure. Lipid content and reproductive allotment were measured for a series of fish from each pond on all dates to explore the energy allocation patterns at the individual. 106 refs., 38 figs., 16 tabs.
Development of an antimycin-impregnated bait for controlling common carp
The common carp Cyprinus carpio is a major problem for fisheries and wildlife managers because its feeding behavior causes degradation of valuable fish and waterfowl habitat. This study was designed to evaluate the effectiveness of an antimycin-impregnated bait for control of common carp. The toxic bait contained fish meal, a binder, antimycin, and water. The ingredients were mixed together and made into pellets. This bait was force-fed to common carp or administered in a pond environment, where fish voluntarily fed on the bait. The lowest lethal dose in the forcefeeding study was 0.346 mg antimycin/kg of fish and doses that exceeded 0.811 mg antimycin/kg were toxic to all fish. On three occasions, adult common carp held in 0.004-ha concrete ponds were offered 10 g of toxic bait containing 5.0, 7.5, and 10 mg antimycin/g of bait and the mean mortalities 96 h later were 21, 35, and 51%, respectively. Three tests were conducted in 0.04-ha earthen ponds each containing 100 adult common carp; these fish were offered 50 g of the toxic bait that contained 10 mg antimycin/g, and the mean mortalities (96 h) were 19, 32, and 74%. Toxic baits should be used in conjunction with other management techniques, and only when common carp are congregated and actively feeding, and when few nontarget bottom- feeding species are present.
Contracaecum sp. larvae (L3) from fish were identified using nucleotide sequences of the internal transcribed spacers ITS-1 and ITS-2 of the ribosomal DNA. The nematode larvae originated from fish in a freshwater situation (crucian carp Carassius carassius, from Selment Wielki Lake in Mazury, northeastern Poland) and a brackish-water region (Caspian round goby Neogobius melanostomus from the Baltic Sea, Gdafisk Bay at the Polish coast). Two strains (Contracaecum rudolphii A and B) of Contracaecum rudolphii senso lato, a parasite common at the adult stage in fish-eating birds, were identified. In fish from the freshwater site, only the strain temporarily designated C. rudolphii B was identified; in the brackish-water region, both strains were found, suggesting that fish serve as paratenic host for both genotypes. Contracaecum rudolphii sensu lato has been recorded in several species of fish-eating birds in Poland, particularly in the great cormorant, Phalacrocorax carbo, in which the abundance is highest. The results, although based on a restricted number of larvae, suggest that the life cycles of both genotypes can be completed in the Polish region and that at least one of them, C. rudolphii B, can develop both in fresh and brackish water. PMID:17918390
Catch-and-release angling is a well-established practice in recreational angler behaviour and fisheries management. Accompanying this is a growing body of catch-and-release research that can be applied to reduce injury, mortality and sublethal alterations in behaviour and physiology. Here, the status of catch-and-release research from a symposium on the topic is summarised. Several general themes emerged including the need to: (1) better connect sublethal assessments to population-level processes; (2) enhance understanding of the variation in fish, fishing practices and gear and their role in catch and release; (3) better understand animal welfare issues related to catch and release; (4) increase the exchange of information on fishing-induced stress, injury and mortality between the recreational and commercial fishing sectors; and (5) improve procedures for measuring and understanding the effect of catch-and-release angling. Through design of better catch-and-release studies, strategies could be developed to further minimise stress, injury and mortality arising from catch-and-release angling. These strategies, when integrated with other fish population and fishery characteristics, can be used by anglers and managers to sustain or enhance recreational fishing resources. ?? 2007 The Authors. Journal compilation 2007 Blackwell Publishing Ltd.
Rapid identification of yeast by fluorescence in situ hybridisation from broth and blood cultures.
Candida species including species other than Candida albicans are of importance as causative agents of sepsis in intensive-care units, requiring prompt initiation of targeted therapy. While fluconazole is usually active against Candida albicans, non-Candida albicans species often require more sophisticated approaches. A rapid species diagnosis is therefore desirable and can be provided by fluorescence in situ hybridisation (FISH). However, broad evaluation studies of described probes are largely lacking and the probe panel that has been described is incomplete. As an addition to previously described C. albicans FISH probes, we evaluated published DNA probes for C. glabrata and C. krusei, as well as newly designed DNA probes for C. krusei, C. lusitaniae, C. parapsilosis, C. tropicalis, Crypotococcus neoformans and a group of intrinsically fluconazole-resistant Candida species for FISH with 22 reference strains, 23 well-characterised laboratory control strains, 169 isolates from clinical samples and 48 blood cultures. Sensitivity and specificity of >99% were demonstrated for all evaluated species-specific probes, whereas the probe that binds to a heterogeneous group of intrinsically fluconazole-resistant Candida species correctly identified eight of nine fluconazole-resistant clinical isolates. FISH yielded reliable results using the classical FISH procedure as well as a recently described slide chamber-based method. Given this good sensitivity and specificity, FISH may be applied for rapid identification of yeast in screening analyses, thus giving the opportunity for more precise targeting of antimycotic therapy. PMID:22681159
Habitat Quality and Anadromous Fish Production on the Warm Springs Reservation. Final Report.
The number of anadromous fish returning to the Columbia River and its tributaries has declined sharply in recent years. Changes in their freshwater, estuarine, and ocean environments and harvest have all contributed to declining runs of anadromous fish. Restoration of aquatic resources is of paramount importance to the Confederated Tribes of the Warm Springs (CTWS) Reservation of Oregon. Watersheds on the Warm Springs Reservation provide spawning and rearing habitat for several indigenous species of resident and anadromous fish. These streams are the only ones in the Deschutes River basin that still sustain runs of wild spring chinook salmon, Oncorhynchus, tshawytscha. Historically, reservation streams supplied over 169 km of anadromous fish habitat. Because of changes in flows, there are now only 128 km of habitat that can be used on the reservation. In 1981, the CTWS began a long-range, 3-phase study of existing and potential fish resources on the reservation. The project, consistent with the Northwest Power Planning Council`s Fish and Wildlife Program, was designed to increase the natural production of anadromous salmonids on the reservation.
This report is the second in a series detailing the procedures used and the results obtained from studies designed to determine the impacts of erosion control structures on fish habitat at Willapa Bay, Washington. The erosion control structure, consisting of a 1600-ft rock groin and an attached 930-ft underwater dike was placed on Washaway Beach in 1998 to protect State Route (SR) 105 from erosion. The objectives of the study are to develop an understanding about whether groin-type structures on the outer coast can alter migratory movement or predation pressure on juvenile and adult salmon. Field surveys in this report were conducted from October 14-21, 2001, and consisted of gillnetting, passive drifter surveys, diver surveys, interviews with fishers and Washington Department of Fish and Wildlife (WDFW) personnel, bird and mammal surveys, and split beam hydroacoustic surveys. Field sampling activities were begun on October 14 and were suspended during the commercial gillnet season from October 16-18. Interviews with fishers and WDFW were conducted during that period, and field sampling recommenced on October 19. The hydroacoustic surveys were conducted from October 19-21. The migration pattern of fish, presumed to be salmon, was documented relative to the tidal phase. Fish were observed to congregate in the deeper portion of the channel during the end of the ebb tide. The fishermen set their nets and rid the tide upstream as they catch fish. Many fewer fish were observed in the channel at the high tide stand.
The investigation was designed to study whether DHA in fish oil encapsulated in porous starch granules coated with water-insoluble corn protein, zein, can be utilized during passage through the gastrointestinal tract in rats. The in vitro digestion trial showed that zein used for coating starch granules was decomposed by proteolytic enzymes. After feeding porous starch granules containing fish oil, coconut oil, or corn oil for 3 weeks, serum triglycerides and phospholipids were significantly lower in the rats on fish oil than in the other rats. The proportion of DHA in total fatty acid of the fish oil group was significantly higher in both liver and brain lipids than in the other two groups. These results clearly show that fish oil encapsulated in porous starch granules coated with zein could be digested and absorbed. Moreover, it was confirmed that the granules had been changed morphologically by scanning electron microscopic analysis of the contents of the alimentary sections, showing that the film of zein had been digested in stomach and small intestine. The digestibility of fish oil in the capsules was shown to be 96.8%.
Interrenal dysfunction in fish from contaminated sites: In vivo and in vitro studies
An endocrine impairment characterized by a reduced capacity to elevate plasma cortisol levels in response to an acute standardized capture stress, has been previously diagnosed in yellow perch, Perca flavescens, and in northern pike, Esox lucius, from sites contaminated by mixtures of pollutants (heavy metals, PAHs and PCBs) or by BKME. The most recent studies were designed to (1) field validate this dysfunction usable as a marker of reduced physiological competence in fish; (2) demonstrate the impairment of the interrenal tissue in fish from sites located in a mining region in Abitibi; and (3) elucidate the physiological mechanisms underlying the impairment of the interrenal tissue in fish chronically exposed to xenobiotics. The responsiveness of the interrenal tissue to a standardized dose of ACTH1-39 was assessed in vivo in yellow perch from contaminated and reference sites maintained in experimental enclosures, as well as in vitro using complete growth medium in a perifusion system and in microplates. The results showed that the functional impairment of the internal tissue in fish exposed to xenobiotics is an exposure related phenomenon modulated by season. The functional tests with the interrenal tissue revealed that the responsiveness to ACTH is significantly reduced and that the synthesis of cortisol is disrupted, in fish from contaminated sites. Use of the functional ACTH-tests with the interrenal tissue of fish in environmental monitoring will be discussed.
An overview of plants used for insect repellents
Introduction In rural areas in Laos, fly larvae infestations are common in fermenting fish. Blowflies (Chrysomya megacephala, Diptera: Calliphoridae) are attracted to oviposit (and/or larviposit) onto fermenting fish which results in infestations with fly larvae. Knowledge of traditional use of plants to repel larvae during the production of fermented fish is common and widespread in Lao PDR. Research Questions How effective are the most salient species in repelling, and killing fly larvae in fermenting fish? Material and Methods The three plant species most frequently reported to repel fly larvae during an ethnobotanical survey throughout Lao PDR were tested for repellence and larvicidal activity of fly larvae infesting fermented fish. The lethality and repellence of Tadehagi triquetrum (L.) H. Ohashi (Fabaceae), Uraria crinita (L.) Desv. ex DC. (Fabaceae) and Bambusa multiplex (Lour.) Raeusch. ex Schult. & Schult. f. (Poaceae) were tested in an experimental design using fermenting fish in Vientiane, Lao PDR. Results The repellent effect of fresh material of T. triquetrum and U. crinita, and the larvicidal effect of fresh B. multiplex, is significantly more effective than that of dried material of the same species, and the total effect (repellence and larvicidal effect combined) for each of the three species was significantly more effective for fresh than for dry material. Fresh material of T. triquetrum, U. crinita, or B. multiplex added on top of the fermenting fish repelled 50%, 54%, 37%, and killed 22%, 28%, and 40% of fly larvae. The total effect was not significantly different per species at 72%, 82%, and 77%, respectively. Discussion and Conclusions The three most salient species are effective in repelling and killing fly larvae in the production of fermented fish, and may be essential to augment food safety during traditional fermentation in open jars. PMID:7771600
Turbulence at Hydroelectric Power Plants and its Potential Effects on Fish.
The fundamental influence of fluid dynamics on aquatic organisms is receiving increasing attention among aquatic ecologists. For example, the importance of turbulence to ocean plankton has long been a subject of investigation (Peters and Redondo 1997). More recently, studies have begun to emerge that explicitly consider the effects of shear and turbulence on freshwater invertebrates (Statzner et al. 1988; Hart et al. 1996) and fishes (Pavlov et al. 1994, 1995). Hydraulic shear stress and turbulence are interdependent natural fluid phenomena that are important to fish, and consequently it is important to develop an understanding of how fish sense, react to, and perhaps utilize these phenomena under normal river flows. The appropriate reaction to turbulence may promote movement of migratory fish or prevent displacement of resident fish. It has been suggested that one of the adverse effects of flow regulation by hydroelectric projects is the reduction of normal turbulence, particularly in the headwaters of reservoirs, which can lead to disorientation and slowing of migration (Williams et al. 1996; Coutant et al. 1997; Coutant 1998). On the other hand, greatly elevated levels of shear and turbulence may be injurious to fish; injuries can range from removal of the mucous layer on the body surface to descaling to torn opercula, popped eyes, and decapitation (Neitzel et al. 2000a,b). Damaging levels of fluid stress can occur in a variety of circumstances in both natural and man-made environments. This paper discusses the effects of shear stress and turbulence on fish, with an emphasis on potentially damaging levels in man-made environments. It defines these phenomena, describes studies that have been conducted to understand their effects, and identifies gaps in our knowledge. In particular, this report reviews the available information on the levels of turbulence that can occur within hydroelectric power plants, and the associated biological effects. The final section provides the preliminary design of an experimental apparatus that will be used to expose fish to representative levels of turbulence in the laboratory.
Objective: Fish oil supplement has been proposed as a non-pharmacological strategy to correct the atherogenic lipid profile associated with type 2 diabetes mellitus. However, fish oil may have deleterious effects on lipid peroxidation and glycemic control.Design: In this study, 44 type 2 diabetic patients were randomized to vitamin E standardized (53.6 mg/day) supplementation (capsules) with 4 g daily of either fish oil (n = 23) or corn oil (n = 21) for 8 weeks preceded by a 4 week run-in period of corn oil supplementation. LDL was isolated by density gradient ultracentrifugation and oxidized in vitro with Cue(2+). As a marker of in vivo oxidation malondialdehyde concentration in LDL (LDL-MDA) was measured.Results: Fish oil reduced both mean lag time (before, 57.8; after, 48.8 min, P <0.001) and mean propagation rate (before, 0.018 &udelta;OD/min; after, 0.015 &udelta;OD/min, P <0.001), whereas corn oil had no influence on lag time and propagation rate. The changes in lag time and propagation rate differed significantly between fish oil and corn oil treatment. LDL-MDA changes differed borderline significantly between groups (FO, 110.4 pmol/mg protein; CO, 6.7 pmol/mg protein; P = 0.057). Fish oil supplementation had no influence on glycemic control as assessed from HbA(1c) and fasting blood glucose.Conclusion: According to our findings, fish oil supplementation leads to increased in vivo oxidation and increased in vitro oxidation susceptibility of LDL particles. More studies are needed to clarify the clinical importance of this finding. Sponsorship: Financially supported by The Danish Heart Association and Dansk Droge A/S. Dansk Droge A/S generously provided the fish oil and corn oil capsules.
The Duck Valley Reservoirs Fish Stocking and Operations and Maintenance (DV Fisheries) project is an ongoing resident fish program that serves to partially mitigate the loss of anadromous fish that resulted from downstream construction of the hydropower system. The project's goals are to enhance subsistence fishing and educational opportunities for Tribal members of the Shoshone-Paiute Tribes and provide resident fishing opportunities for non-Tribal members. In addition to stocking rainbow trout (Oncorhynchus mykiss) in Mountain View, Lake Billy Shaw, and Sheep Creek Reservoirs, the program is also designed to maintain healthy aquatic conditions for fish growth and survival, to provide superior facilities with wilderness qualities to attract non-Tribal angler use, and to offer clear, consistent communication with the Tribal community about this project as well as outreach and education within the region and the local community. Tasks for this performance period are divided into operations and maintenance plus monitoring and evaluation. Operation and maintenance of the three reservoirs include fences, roads, dams and all reservoir structures, feeder canals, water troughs and stock ponds, educational signs, vehicles and equipment, and outhouses. Monitoring and evaluation activities included creel, gillnet, wildlife, and bird surveys, water quality and reservoir structures monitoring, native vegetation planting, photo point documentation, control of encroaching exotic vegetation, and community outreach and education. The three reservoirs are monitored in terms of water quality and fishery success. Sheep Creek Reservoir was very unproductive this year as a fishery. Fish morphometric and water quality data indicate that the turbidity is severely impacting trout survival. Lake Billy Shaw was very productive as a fishery and received good ratings from anglers. Mountain View was also productive and anglers reported a high number of quality sized fish. Water quality (specifically dissolved oxygen and temperature) is the main limiting factor in our fisheries.
Fish mortality as a function of spillway characteristics
An extensive review of published and unpublished information on fish mortality as a function of spillway characteristics is presented, along with design and operating suggestions that will eliminate or reduce the impact of Canadian spillways on fish. Juveniles of most fish species will pass downstream, over or through spillways if outlet flow is sufficient. Fish passing over relatively high spillways may be injured by rapid pressure changes, rapid deceleration, shearing forces, turbulence, abrasion and the striking force on the water in free fall. Gas-bubble disease in fish can occur when air entrapment supersaturates river water below spillways. Water cascading over spillways drives air to a depth sufficient to result in solubility within the water greater than the solubility at normal atmospheric pressure. Several species of salmonids are reluctant to sound to submerged outlets at dams, especially if the outlets are located at depths greater than 20 m. Flow deflectors placed near the base of spillways have been successful in reducing the deep plunging action which is responsible for air entrainment and subsequent nitrogen supersaturation. Ski-jump spillways have been successful in eliminating injuries caused by fish striking the face of the dam. To obtain highest survival for downstream migrating fish, spillways should be operated for minimum turbulence, back-roll and energy dissipation per unit of spillway width. The direct screening of spillway flows to prevent fish entrainment is not currently practised and appears to be either unjustified (in the case of spillways less than ca 35 m in height), or impractical where large volumes of flow are involved. 109 refs., 11 figs., 4 tabs.
Development of a more fish-tolerant turbine runner, advanced hydropower turbine project
Alden Research Laboratory, Inc. (ARL) and Northern Research and Engineering Corporation (NREC) conducted a research program to develop a turbine runner which will minimize fish injury and mortality at hydroelectric projects. ARL?NREC have developed a runner shape which minimizes the number of blade leading edges, reduces the pressure versus time and the velocity versus distance gradients within the runner, minimizes or eliminates the clearance between the runner and runner housing, and maximizes the size of the flow passages, all with minimal penalty on turbine efficiency. An existing pump impeller provided the starting point for developing the fish tolerant turbine runner. The Hidrostal pump is a single bladed combined screw/centrifugal pump which has been proven to transport fish with minimal injury. The focus of the ARL/NREC research project was to develop a new runner geometry which is effective in downstream fish passage and hydroelectric power generation. A flow of 1,000 cfs and a head in the range of 75 ft to 100 ft were selected for conceptual design of the new runner. Conceptual design of the new runner began with a re-evaluation of studies which have been previously conducted to identify probable sources of injury to fish passing through hydraulic turbines. Criteria relative to hydraulic characteristics which are favorable for fish passage were prepared based on a reassessment of the available information. Important criteria used to develop the new runner design included low pressure change rates, minimum absolute pressures, and minimum shear. Other criteria which are reflected in the runner design are a minimum number of blades (only two), minimum total length of leading edges, and large flow passages. 86 figs., 5 tabs.
Fisheries management in brazilian reservoirs: Lessons from/for South America
Abstract in portuguese Nas últimas décadas, as principais ações tomadas em relação ao manejo da pesca em reservatórios brasileiros (controle, estocagem e construção de escadas de peixe) foram, em geral, ineficientes. As razões do insucesso podem ser atribuídas à escassez de informações, limitações de recursos financeiros e humanos e, a falta de monitoramento. Estas ações foram, aparentemente, baseadas em um paradigma ("deserto biológico"), que se revelou equivocado. A tendên (more) cia de pautar as ações conforme um novo paradigma, agora vigente ("biomanipulação"), revela-se promissora. Esse novo paradigma incorpora uma visão abrangente do sistema de pesca (o ambiente, o pescador e o peixe) e pressupõe monitoramento e retroalimentação. Além disso, para que o manejo seja efetivo, deve considerar o conceito de bacia hidrográfica e usos múltiplos, reconhecendo as limitações biológicas, políticas e sócio-econômicas no processo decisório. Abstract in spanish En las últimas décadas, las principales acciones tomadas en relación al manejo pesquero en embalses brasileros (control, stock y construcción de escaleras para peces) fueron, en general, ineficientes. Las razones que condujeron a esta ineficiencia pueden ser atribuídas a la escasez de información, limitación de recursos financieros y humanos, y a la falta de monitoreos. Estas razones fueron, aparentemente, basadas en un paradigma ("desierto biológico"), que luego (more) se demostró equívoco. La tendencia de basar acciones conforme un nuevo paradigma, actualmente vigente ("biomanipulación"), se muestra promisoria. Este nuevo paradigma incorpora una visión más holística de las pesquerías (el ambiente, el pescador, el pez) y presupone monitoreo y retroalimentación. Además, para que el manejo sea efectivo, se debe considerar el concepto de cuenca hidrográfica y el de uso múltiple, reconociendo las limitaciones biológicas, políticas y socio económicas en el proceso decisivo. Abstract in english In the last decades, main actions related to fisheries management in Brazilian reservoirs (regulations, stocking, and construction of fish ladders) were, in general, not successful. Reasons for that were shortage of scientific information, limitations of financial and human resources, and absence of monitoring. Apparently these actions were based on a paradigm ("biological deserts") which revealed itself misleading. The tendency of supporting the actions according to the (more) ongoing paradigm ("biomanipulation") seems to be promising. This paradigm incorporates a more holistic view of the fisheries (environment, fishermen and fish) and considers monitoring and feedback. Moreover, for an effective management the concept of hydrographic basins and multiple uses must be considered, recognizing biological, political, and socio-economic limitations in the decision making process.
Movement patterns of the parrotfish Sparisoma cretense in a Mediterranean marine protected area.
Understanding the movements of exploited fish has become a major concern for several management and conservation initiatives, such as the implementation of well designed marine protected areas. Movements of an important recreational target species from the Mediterranean Sea, the parrotfish Sparisoma cretense, were determined in a marine protected area using passive acoustic telemetry, to evaluate site fidelity and homing, quantify home range and identify temporal patterns. Six adult parrotfish (20.8-29.8 cm total length) were caught along the north-eastern coast of Lampedusa (Italy) and surgically implanted with acoustic transmitters. Three fish were caught and released at the same site within the integral (i.e. "no entry-no take") reserve, whereas the others were caught outside the reserve boundary and released at the mid reserve 0.5 km apart. Two of the three fish that were released away from the capture site demonstrated homing abilities. Four fish showed a strong site fidelity, whereas the others moved frequently in and out of the monitoring area. Home range sizes estimated over the period of monitoring (up to 207 days) varied from 70,387 to 256,398 m(2), with core areas of 16,688 to 84,946 m(2). Home range size did not differ significantly between day and night. Home ranges of all fish extended beyond the reserve boundaries, showing example of potential spillover into take zones. Temporal activity patterns of fish were diurnal with a dominant diel rhythm, likely due to a resting behaviour at night. Though not specifically designed to protect exploited fish, the marine reserve of Lampedusa seems adequate for the conservation and management of S. cretense. PMID:23069664
Motivating conservation: Learning to care for other species in a local ecological community
Large-scale, sustainable biodiversity conservation must motivate action by local communities. I united theories and practices in biology and psychology to study the process by which people are motivated to care for other species, and to what extent caring results in helping. Participants (N = 1200), age 8--22, interacted with native fish and aquatic insects in their habitats during 21 field experiences through Lake County, Montana educational institutions. Native fish were chosen because they are familiar to local people, yet different from people in their morphology, biomechanics, and habitat. In Phase I, two activity models for conservation emerged: the Habitat approach linked concepts in ecology, reciprocation, and a moral orientation toward justice, while the Behavior approach linked concepts in behavior, kin selection, and a moral orientation toward caring. These two approaches were compared in Phase II through seven sets of experiences that varied only in point of view: toward the habitat or toward behavior. I found that through sustained contact between people and local fish in their habitats, in the field and in cold-water aquaria, people empathized with fish more than with habitats. They perceived fish states by interpreting their behavior, and created meaning by focusing on fish social interactions with their habitat, with other fish, and with people. They used the information gained from empathy to identify ongoing conservation needs and to design conservation plans. Attention to behavior increased perception of human impacts on fish; perception of relatedness with fish; similarity with the physiology, behavior, minds and lives of fish; desire for non-material benefits in return for helping fish; and cohesion within participant groups. These perceptions varied with age and gender. For example, women and children emphasized values of non-material returns for time invested. This study recommends a behavioral-ecology approach for motivating conservation and environmental education in local communities, that begins by observing human impacts on the behaviors of other species. Using this approach will increase the likelihood of creating extensive relationships among social-ecological communities and thus increase non-material benefits in exchange for conservation costs. It is also essential to involve women and children in decision-making to help ensure multi-generational ecological sustainability.
Temporary Restoration of Bull Trout Passage at Albeni Falls Dam
This study was designed to monitor movements of bull trout that were provided passage above Albeni Falls Dam, Pend Oreille River. Electrofishing and angling were used to collect bull trout below the dam. Tissue samples were collected from each bull trout and sent to the U. S. Fish and Wildlife Service Abernathy Fish Technology Center Conservation Genetics Lab, Washington. The DNA extracted from tissue samples were compared to a catalog of bull trout population DNA from the Priest River drainage, Lake Pend Oreille tributaries, and the Clark Fork drainage to determine the most probable tributary of origin. A combined acoustic radio or radio tag was implanted in each fish prior to being transported and released above the dam. Bull trout relocated above the dam were able to volitionally migrate into their natal tributary, drop back downstream, or migrate upstream to the next dam. A combination of stationary radio receiving stations and tracking via aircraft, boat, and vehicle were used to monitor the movement of tagged fish to determine if the spawning tributary it selected matched the tributary assigned from the genetic analysis. Seven bull trout were captured during electrofishing surveys in 2008. Of these seven, four were tagged and relocated above the dam. Two were tagged and left below the dam as part of a study monitoring movements below the dam. One was immature and too small at the time of capture to implant a tracking tag. All four fish released above the dam passed by stationary receivers stations leading into Lake Pend Oreille and no fish dropped back below the dam. One of the radio tags was recovered in the tributary corresponding with the results of the genetic test. Another fish was located in the vicinity of its assigned tributary, which was impassable due to low water discharge at its mouth. Two fish have not been located since entering the lake. Of these fish, one was immature and not expected to enter its natal tributary in the fall of 2008. The other fish was large enough to be mature, but at the time of capture its sex was unable to be determined, indicating it may not have been mature at the time of capture. These fish are expected to enter their natal tributaries in early summer or fall of 2009.
The environmental impacts of three different queen scallop (Aequipecten opercularis) fishing gears.
The negative impact of demersal fishing gears on the marine environment may be mitigated by utilizing less damaging fishing gears. Within this context three queen scallop fishing gears were tested for their catch efficiencies and their environmental impact on benthos: a traditional 'Newhaven' dredge, a new dredge design with a rubber lip instead of the traditional teeth as its main new design feature and an otter trawl. Both, the new dredge and the otter trawl showed high catches and relatively low by-catches. Catches made with the traditional dredge were lower and contained larger amounts of non-target species. Both dredges primarily caught invertebrate species, while by-catches of the otter trawl were dominated by demersal fish. The impact of these gears on the benthic biota demonstrated that while no effects were detected for the otter trawl both dredges showed similar negative effects. Clear negative effects were evident for the brittlestar Ophiura ophiura while positive trends for the common starfish Asterias rubens and the hermit crab Pagurus bernhardus indicated scavenging effects. Due to its higher catch efficiency the new dredge caused less damage per unit catch compared to the traditional dredge, yet compared to the otter trawl it appears less environmentally friendly. However, the new dredge may be an alternative to the otter trawls for fisheries where the by-catch of demersal fish has been identified as a significant problem. PMID:22119657
Behavioral and kinematic properties and capacities of wild migratory salmonid fishes swimming upstream and jumping up waterfalls generally have played only minor roles in the design and construction of passageways intended to help these fishes get past dams and other human-made obstacles blocking their movements. This paper reports the results of an experimental study of relevant behavioral and kinematic properties of adult kokanee salmon (Oncorhynchus nerka) jumping up waterfalls as they migrate upstream. We used a portable, adjustable apparatus to study in the field fish responding to artificial waterfalls under a range of flow conditions. We observed fish under conditions of varying water flow rates, pool depths, fall heights and fall angles. We analyzed digital video recordings of their behaviors. Kokanee salmon spontaneously jump up waterfalls within a relatively narrow range of conditions, including low flow speeds, near vertical angles and pool depth to fall height ratios near 1.0. Preferred values for each parameter are, to some extent, dependent on other parameters. In contrast to previous misconceptions, jumping behavior is initiated by running S-start accelerations from beneath the boils formed in the plunge pools below waterfalls, as opposed to C-start standing jumps from the surface. S-starts are immediately followed by burst swimming to the point of takeoff at the surface. These results can contribute to an improved basis for developing designs of fish passageways that may ultimately make them more effective and efficient.
Effects of channel incision on base flow stream habitats and fishes
Channel incision is a widespread phenomenon that results in stream and riparian habitat degradation. Fishes and physical habitat variables were sampled at base flow from three incised stream channels and one reference stream in northwest Mississippi, USA, to quantify incision effects on fish habitat and provide a basis for habitat rehabilitation planning and design. Incised channels were sampled in spring and autumn; the reference channel was sampled only in the autumn. Incised channel habitat quality was inferior to the reference channel despite the presence of structures designed to restore channel stability. Incised channels had physical habitat diversity levels similar to a nonincised reference channel, but contained fewer types of habitat. At base flow, incised channels were dominated by shallow, sandy habitats, moderate to high mean local Froude numbers, and had relatively little organic debris in their beds. In contrast, the reference stream had greater mean water depth, contained more woody debris, and provided more deep pool habitat. Fish assemblages in incised channels were composed of smaller fishes representing fewer species relative to the reference site. Fish species richness was directly proportional to the mean local Froude number, an indicator of the availability of pool habitat.
Is fish passage technology saving fish resources in the lower La Plata River basin?
Abstract in portuguese Mais de 450 barragens foram construídas no alto rio Paraná, Brasil, nos últimos 40 anos. A regulação dos rios por barragens é considerada um dos fatores primários de redução da diversidade de peixes e depleção de espécies migratórias. Em contraste, somente duas grandes barragens foram construídas nos trechos mais inferiores da bacia do rio da Prata. No momento, há uma grande preocupação acerca do impacto dos represamentos sobre espécies que se constituem (more) a base da pesca comercial e esportiva na bacia. Este artigo aborda o desempenho das passagens de peixes das barragens de Yacyreta e Salto Grande, monstrando que as mesmas falham na transferência de grandes quantidades de espécies migratórias para os trechos a montante. A barragem de Yacyreta tem dois elevadores com problemas importantes de projeto. Como resultado, a eficiência na passagem das espécies-alvo é menor do que 2%. A diversidade das espécies transferidas é baixa, uma vez que 85% dos espécimes dominantes nos elevadores foram representadas por uma espécie de Pimelodidae (Pimelodus maculatus) e duas de Doradidae (Oxydoras kneri e Rhinodoras dorbignyi). A passagem de grandes espécies alvo migratórias raramente compreende até 5% do número de peixes no sistema de passagem. A barragem de Salto Grande tem duas passagens do tipo eclusas de Borland linstaladas, cuja performance varia consideravelmente dependendo da operação da casa de força e dos vertedouros, bem como da elevação de nível no canal de fuga. A maioria dos espécimes que passam pertencem a uma pequena espécie de Pimelodidae (Parapimelodus valenciennis) e a uma espécie de Engraulidae (Lycengraulis grossidens). Um novo direcionamento no desenho de passagens de peixes é necessário para a preservação das espécies migratórias em rios Sul Americanos. Informações integradas de geomorfologia (habitat), atratores de natureza hidrodinâmica detectável pelos peixes para selecionar as rotas de migração ascendentes e descendentes, capacidade natatória dos peixes e estudos detalhados de populações são necessárias. Abstract in english Over 450 dams have been constructed in the upper Paraná River basin in Brazil during the past 40 years. River regulation by these dams is considered a primary factor in the reduction of fish diversity and depletion of migratory species. In contrast to the upper Paraná Basin, only two large dams (both with upstream fish passage) have been constructed in the lower La Plata River basin. Fishery managers in the lower basin are concerned that existing and planned dams will f (more) urther deplete populations of migratory fish species that constitute important recreational and commercial fisheries as has occurred in the upper basin. We assessed the sustainability of fisheries in the lower basin in the face of increased river regulation by using literature information to describe the efficiency of the fish passage systems used to mitigate river regulation impacts on fisheries. Our analysis shows that fish passage systems at both lower basin dams, Yacyreta and Salto Grande, fail to transfer sufficient numbers of upstream migrants to sustain populations of migratory species. Fish passage efficiency of target species in the fish elevators at Yacyreta is less than 2%. Fish diversity in the fish elevators is low because about 85% of the fish belong to only three non-migratory species (Pimelodus maculatus, Oxydoras kneri and Rhinodoras dorbignyi). Large migratory species targeted for passage rarely comprise even 5 % of the fish number in the passage system. The two Borland locks at Salto Grande Dam cannot dependably pass large numbers of migratory species because passage efficiency is dependent upon interactions of powerhouse and spillway operation with tailrace elevations. Most species in the Borland system were either a small catfish (Parapimelodus valenciennis) or a engraulid (Lycengraulis grossidens). Again, the targeted migratory species were not abundant in the passage system. We conclude that existing fish passage technology in the lower basin is inadequate and that improved fish passage designs are required to conserve migratory species. These designs must be based on integrated information from geomorphology (habitat), natural fish behavior, fish swimming capabilities, and detailed population studies.
Although histopathology is routinely employed as a tool for the detection and assessment of xenobiotic-mediated effects in mammals, it is less frequently applied to fish. In part, this is due to a lack of method standardization regarding study design, tissue preservation, tissue sectioning, histopat...
Fillet weight is an economically important trait in Nile tilapia production for the European market which asks for fish with average body weights of at least 700 g. Genetic parameters to design or optimize breeding programs for these body weights are lacking. In an earlier study we showed that high ...
Two fission yeast temperature-sensitive mutants, cut6 and lsd1, show a defect in nuclear division. The daughter nuclei differ dramatically in size (the phenotype designated lsd, large and small daughter). Fluorescence in situ hybridization (FISH) revealed that sister chromatids were separated in the...
Southern Idaho Wildlife Mitigation Implementation 2000 Annual Report.
This report covers calendar year 2000 activities for the Southern Idaho Wildlife Mitigation Implementation project. This project, implemented by Idaho Department of Fish and Game and Shoshone Bannock Tribes wildlife mitigation staff, is designed to protect, enhance and maintain wildlife habitats to mitigate construction losses for Palisades, Anderson Ranch, Black Canyon and Minidoka hydroelectric projects. Additional project information is available in the quarterly reports.
Southern Idaho Wildlife Mitigation Implementation 2000 Annual Report.
This report covers calendar year 2001 activities for the Southern Idaho Wildlife Mitigation Implementation project. This project, implemented by Idaho Department of Fish and Game and Shoshone Bannock Tribes, is designed to protect, enhance and maintain wildlife habitats to mitigate for construction losses associated with Anderson Ranch, Black Canyon, Deadwood, Minidoka and Palisades hydroelectric projects. Additional project information is available in the quarterly reports.
Molecular data show that the filamentous bacterium Eikelboom type 0092, frequently seen in Australian activated sludge plants, is a member of the phylum Chloroflexi. Fluorescence in situ hybridization (FISH) probes designed against cloned 16S rRNA sequences from a full-scale enhanced biological phos...
Hydraulic design of a tilting weir allowing for periodic fish migration
The hydraulic design of a tilting weir is presented, which allows for periodic exchange of potadromous fish between freshwater ecosystems. The application domain includes inland waters that need to be isolated hydraulically, preserving the existing ecological connection with the surrounding areas as...
Feeding experiment is designed on the basis of food allowance rather than feed formulation. Food allowances are fixed in the attempt to provide the desired amount of nutricients per body mass of fish per day (in terms of g.kg-1.d-1 or J.kg-1.d-1). By this way, previous determination of optimum feedi...
The combined use of chemical and biochemical markers to assess water quality along the Ebro River
10 pages, 4 figures, 4 tables. | Carp (Cyprinus carpio) and barbel (Barbus graellsii) were collected from five sites along the Ebro River. The study was designed to assess levels of persistent organic pollutants and metals bioaccumulated by fish, and some biochemical responses (cytochrome P450 syste...
Vertebrate behavior and ecology. Progress report, July 1, 1976--June 30, 1977
Progress is summarized under six subprojects; engineering design and development; statistical procedures and quantitative methods for analysis of ecological and behavioral data; coexistence and population dynamics of selected vertebrates; application of radiotelemetry to selected problems in vertebrate censusing and population study; fish response to alterations in water quality resulting from power production; and seasonal migrations and habitat selection of the pronghorn antelope (Antilocapra americana).
The effects of essential fatty acid deficiency and temperature on the fatty acid profiles of polar lipids (PL) and neutral lipids (NL) from various tissues (muscle, liver, gills, eyes and brain) of European sea bass juveniles were compared in a two factorial design. Fish (60 g) were held for 84 days...
Dams designed for hydropower and other purposes alter the environments of many economically important fishes, including Chinook salmon (Oncorhynchus tshawytscha). We estimated that dams on the Rogue River, the Willamette River, the Cowlitz River, and Fall Creek decreased water temperatures during su...
Walleye discrete epidermal hyperplasia (WEH) is a hyperproliferative skin disease that is prevalent on adult walleye fish throughout North America. We have identified two retroviruses associated with WEH, designated here as walleye epidermal hyperplasia virus type 1 and type 2 (WEHV1 and WEHV2), tha...
Humpback Creek supplies power to Alaskan village
This article describes the design and installation of a small (1.25-MW) hydro plant in Cordova, Alaska, a small, isolated fishing village. The plant supplies 15-25% of the town's power, and has reduced dependende on diesel oil. The hydro plant is operated remotely from one of two diesel generation stations 7 miles away.
A review and synthesis of existing information were used to develop a Habitat Suitability Index (HSI) model for the diamondback terrapin (Malaclemys terrapin). The model is scaled to produce an index between 0.0 (unsuitable habitat) and 1.0 (optimum habitat). HSI models are designed to be used with Habitat Evaluation Procedures previously developed by the US Fish and Wildlife Service.
Client Service Centre Building : Sketchup model
Known colloquially as the ‘Fish Bowl’ because of its all-glass exterior, the building was designed by UP alumnus Samuel Pauw to fit between the split-legged construction of the Human Sciences building. Construction took place in 2001 and 2002. The building features elements of Neo-Industrialism. The...
The Canal da Piracema at Itaipu Dam as a fish pass system
Abstract in portuguese O Canal da Piracema é considerado o maior sistema de transposição para peixes no mundo, com 10 km de extensão. A sua construção foi controversa, pois viabilizou a conexão entre duas províncias ictiofaunísticas distintas. Este trabalho buscou avaliar a ictiofauna presente no Canal da Piracema, a abundância e distribuição das espécies migradoras de longa distância ao longo deste sistema de transposição (indicação de seletividade). O Canal da Piracema mostr (more) ou-se difícil de ser amostrado, devido a sua elevada heterogeneidade ambiental: lagos artificiais, escadas e canais semi-naturais, sendo utilizados diversos aparelhos de pesca, adequados aos diversos biótopos (litoral não estruturado e estruturado, amostrados com redes de arrasto e pesca elétrica; lêntico, amostrado com redes de espera e espinhel (mais profundo); e águas rápidas, amostradas com tarrafas). A ictiofauna presente seguiu o padrão para a América do Sul e rio Paraná, com amplo domínio de Characiformes e Siluriformes, sendo Characidae, Anostomidae, Pimelodidade e Loricariidae as famílias mais representativas. Ao todo foram capturadas 116 espécies sendo que 17 eram migradoras de longas distâncias. Nas regiões litorâneas estruturadas e não estruturadas do Canal da Piracema, as capturas se restringiram às espécies de pequeno porte, sendo as mais representativas Bryconamericus exodon e Apareiodon affinis.Nas áreas lênticas houve predomínio de Hypostomus spp., seguidos de Iheringichthys labrosus. Nas áreas lênticas profundas a espécie mais capturada foi Hoplias aff. malabaricus. Nas áreas de águas rápidas houve maior captura de espécies migradoras de longa distância, sendo Prochilodus lineatus e Leporinus elongatus as mais capturadas. A redução acentuada, no sentido de jusante a montante, inclusive das migradoras, indica que o Canal da Piracema está sendo seletivo. Então, a busca de informações dos vários tipos de sistemas de transposição existentes ao longo do Canal da Piracema é fundamental para permitir a movimentação ascendente de peixes. Se isto for alcançado, este polêmico mecanismo de transposição tem potencial para contribuir para a conservação dos estoques de peixes no reservatório de Itaipu e segmento a montante, devido a presença de áreas de desova e crescimento necessários para as espécies migradoras. Abstract in english The Canal da Piracema is the longest (nearly 10 km) fish pass system in the world. The construction of this fish pass was somehow controversial, because it connected two distinct ichthyofaunistic provinces. This study evaluated the ichthyofauna present in the Canal da Piracema and the abundance and distribution of long-distance migratory fish species along this fish pass system (evaluated possible selectivity). The Canal da Piracema was shown to be difficult to sample bec (more) ause of its environmental heterogeneity: artificial ponds, ladders and nature-like fish pass. To solve this problem, we used several fishing gears, adequate for the several biotopes present (unstructured and structured littoral were sampled with seining nets and electrofishing; lentic were sampled with gillnets and longlines (deeper areas); and rapid water areas were sampled with cast nets). The ichthyofauna of the Canal da Piracema followed the pattern for South America and the Paraná River, with a predominance of Characiformes and Siluriformes. The most representative families were Characidae, Anostomidae, Pimelodidae and Loricariidae. We captured 116 species (17 were long-distance migratory) during the period studied. Small-sized species were predominant in unstructured and structured littoral areas, especially Bryconamericus exodon and Apareiodon affinis.The most abundant species was Hypostomus spp. in lentic areas, followed by Iheringichthys labrosus. Hoplias aff. malabaricus predominated in deeper lentic areas. Long-distance migratory species were abundant in rapid waters; they were Prochilodus lineatus and Leporinus elongatus. The sharp reduction in the number of species, including migratory ones, is an indication that the Canal da Piracema is selecting the species that ascend it. Therefore, the search for information on the efficiency of the various fish passes present in the Canal da Piracema is fundamental, to facilitate upward movements of fish. If this is reached, this polemic fish pass has the potential to contribute to the conservation of fish stocks in Itaipu Reservoir and upstream stretches, because of the presence of spawning and development (nurseries) areas for migratory species.
D0 Silicon Upgrade: Thermal Analysis of the D0 Double Sided Ladders
A side view of the double sided ladder is shown in Figure 1. There are two types of double sided ladder; 6 chip and 9 chip. The 6 chip ladder has three SVX II chips mounted directly opposite the cooling channel and 3 chips mounted at the ladder end. The 9 chip ladder has 4 SVX II chips directly opposite the cooling channel and 5 chips at the ladder end. The power density is highest in the 6 chip ladder. All plots and calculations in this Engineering Note pertain to the 6 chip ladder with the understanding that the 9 chip temperature profile is somewhat improved over the 6 chip due to the reduced power density in the row of chips opposite the cooling channel. The two dimensional finite difference technique used for these calculations is described in DOEN 447 and will not be described here. The assumed thermal conductivity of beryllium is 190 W/m-K, and that of silicon is 149 W/m-K. The SVX II power dissipation is assumed 0.400 W. There is no cable or hybrid component power dissipation in this set of simulations. The epoxy in the glue joints consists of 2 mils thickness of thermally conductive epoxy with 0.8 W/m-K conductivity and 1 mil thickness of 0.22 W/m-K unfilled epoxy. The assumed gas temperature outboard of the bulkhead is 15 C with a convection coefficient of 5 W/m{sup 2}-K. The cooling channel temperature is 0 C. The intention of this series of simulations is to determine the optimum thickness to achieve an acceptable silicon temperature during operation of the ladder. Once the operating temperature profile is known, the temperatures within the beryllium substrates are used as input for an ANSYS* calculation of the bow of the ladder during operation. A series of calculations have been performed over the last several months in order to consider the effect of thickness, chip power, gas temperature, etc. on the temperature profile. Only the final plots are shown here in runs 660, 661, and 662. In these three plots the conditions are identical except for the beryllium substrate thickness which is 0.3/0.3, 0.4/0.4, and 0.5/0.3 bottom/top beryllium thicknesses, which are in dimensions of mm.
ETHNOPHARMACOLOGICAL RELEVANCE: Wattakaka volubilis has been traditionally used in Ayurvedic medicine in India for treatment of several ailments such as bronchial asthma, inflammations, tumors, piles, leucoderma, application to boils, rat bite etc. AIM OF THE STUDY: The present study was designed to investigate anti-leukemic activity of the crude aqueous methanolic extract and to identify active compounds from the leaves of Wattakaka volubilis. MATERIALS AND METHODS: The leaves of Wattakaka volubilis were extracted with aqueous methanol. Liquid-liquid fractionation of the crude methanolic extract with different organic solvents was done and the fractions were screened for in vitro anti-leukemic activity using different leukemic cell lines. The active fractions were then subjected to chromatographic separation for isolation of bioactive compounds. Structure of isolated compound was elucidated by spectroscopic methods. The in vitro anti-leukemic activities of different extracts of the leaves and isolated compound WVP were studied in U-937, HL-60 and K-562 cell-lines by using cell count, MTT [(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] and DNA laddering assays, flow-cytometric and confocal microscopic techniques. RESULTS: Kaempferol-3-O-[?-l-rhamnopyranosyl-(1?4)-O-?-l-rhamnopyranosyl-(1?6)-O]-?-d-glucopyranoside (WVP) was isolated from crude leaves extract of Wattakaka volubilis. Both the n-butanolic extract (WVB) of Wattakaka volubilis and its isolate WVP were found to be responsible for in vitro anti-leukemic activity. The IC(50) values of WVB were found be 120, 100 and 50(?g/ml) in U937, K562, and HL-60 cell lines, respectively. Whereas, the pure isolate WVP exhibited anti-leukemic activity with IC(50) values of 13.5, 10.8, and 13.2(?g/ml) in U937, K562, and HL-60 cell lines, respectively. The flow-cytometric analysis confirms that the cell cycle arrest occurs at G1 phase in case of U937 and K562 cell lines and G2/M phase in case of HL60 cell lines. Similarly both confocal microsocopic analysis and DNA laddering assay confirm the apoptosis and cell cycle arrests of leukemic cells. CONCLUSION: The overall results provide evidence for the ethnopharmacological relevance for use of the plant Wattakaka volubilis in developing novel agents for the treatment of leukemia. PMID:23069944
Restoration plans for Metzger Marsh, a coastal wetland on the south shore of western Lake Erie, incorporated a fish-control system designed to restrict access to the wetland by large common carp (Cyprinus carpio). Ingress fish passageways in the structure contain slots into which experimental grates of varying size and shape can be placed to selectively allow entry and transfer of other large fish species while minimizing the number of common carp to be handled. We tested different sizes and shapes of grates in experimental tanks in the laboratory to determine the best design for testing in the field. We also tested northern pike (Esox lucius) because lack of access to wetland spawning habitat has greatly reduced their populations in western Lake Erie. Based on our results, vertical bar grates were chosen for installation because common carp were able to pass through circular grates smaller than body height by compressing their soft abdomens; they passed through rectangular grates on the diagonal. Vertical bar grates with 5-cm spacing that were installed across much of the control structure should limit access of common carp larger than 34 cm total length (TL) and northern pike larger than 70 cm. Vertical bar grates selected for initial field trials in the fish passageway had spacings of 5.8 and 6.6 cm, which increased access by common carp to 40 and 47 cm TL and by northern pike to 76 and 81 cm, respectively. The percentage of potential common carp biomass (fish seeking entry) that must be handled in lift baskets in the passageway increased from 0.9 to 4.8 to 15.4 with each increase in spacing between bars. Further increases in spacing would greatly increase the number of common carp that would have to be handled. The results of field testing should be useful in designing selective fish-control systems for other wetland restoration sites adjacent to large water bodies.
Turbulence in gravel bed rivers plays a critical role in most stream processes including contaminant and nutrient transport, aquatic habitat selection, and natural channel design. While most hydraulic designs and fluid models are based on bulk velocity, migrating juvenile salmon experience and react to the temporally varied turbulent fluctuations. Without properly understanding and accounting for the continuous turbulent motions proper fishway design and guidance are impossible. Matching temporally varied flow to fish reactions is the key to guiding juvenile salmonids to safe passageways. While the ideal solution to fish guidance design would be to use specific fluid action-fish reaction mechanisms, such concrete cause and effect relations have not been established. One way to approach the problem of guidance is to hypothesize that in an environment lacking obvious bulk flow cues (like the reservoir environment), turbulent flow conditions similar to those experienced by juvenile salmonids in natural migration corridors will be attractive to juvenile salmonids. Proof of this hypothesis requires three steps: (1) gathering data on turbulence characteristics in natural migration corridors, (2) reproduction of the turbulence parameters in a controlled environment, and (3) testing the reproduced turbulence on actively migrating juvenile salmonids for increased passage efficiencies. The results from the third step have not been finalized, therefore this report will focus on understanding turbulent processes in gravel bed rivers and reproduction of turbulence in controlled environments for use in fish passage technologies. The purposes of this report are to (1) present data collected in natural gravel bed rivers, (2) present a simple method for reproduction of appropriate turbulence levels in a controlled environment, (3) compare these results to those from one prototype surface collector (PSC), and (4) discuss the implications on fish passage design.
