factor antibody suppresses: Topics by WorldWideScience.org

T cell-engaging BiTE antibodies specific for EGFR potently eliminate KRAS- and BRAF-mutated colorectal cancer cells

Most antibodies to factor VIII have recently been shown to react with discrete regions of the factor VIII light chain (within the C2 domain) and/or the factor VIII heavy chain (within the amino-terminal...Full Text Available

1989-06-01

2

On the effect of ionizing radiation on some factors of hamster natural immunity in norm and in himenolepis diminuta invasion

Epidermal growth factor receptor (EGFR)-specific monoclonal antibodies predominantly inhibit colorectal cancer (CRC) growth by interfering with receptor signaling. Recent analyses have shown that patients...Full Text Available

2010-07-13

3

Predictive and prognostic markers for epidermal growth factor receptor inhibitor therapy in non-small cell lung cancer

Effect of single and chronic irradiation on factors of hamsters natural immunity such as complement, lysozyme and antibodies in norm and in the cestode invasion has been investigated. Significant phase changes in the complement activity level, lysozyme content and specific antibodies tite have been shown to occur in hamster blood under the influence of ionizing radiation. Cestode invasion in irradiated host organism increases the negative effect of ionizing radiation on the hamster immyne system.

1994-01-01

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Global suppression of mitogen-activated ovine peripheral blood mononuclear cells by surface protein activity from Mycoplasma ovipneumoniae

Epidermal growth factor receptor (EGFR) related therapies – mainly tyrosine kinase inhibitors (TKIs) such as erlotinib and gefitinib, but also monoclonal antibodies targeting EGFR, for example,...Full Text Available

2009-11-01

5

British Library Electronic Table of Contents (United Kingdom)

Mycoplasma ovipneumoniae is associated with chronic non-progressive pneumonia of sheep and goats. As with many other mycoplasmas involved in animal diseases, protective immune responses have not been achieved with vaccines, even though antibody responses can be obtained. This study focuses on characterizing the interaction of M. ovipneumoniae with ovine PBMC using carboxy-fluorescein-succinimidyl-ester (CFSE) loading and flow cytometry to measure lymphoid cell division. M. ovipneumoniae induced a strong in vitro polyclonal suppression of CD4^+, CD8^+, and B blood lymphocyte subsets. The suppressive activity could be destroyed by heating to 60^oC, and partially impaired by formalin and binary ethyleneimine treatment that abolished its viability. The activity resided on the surface-exposed m...

2010-01-01

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DIMINISHED DEGRADATION OF MYELIN BASIC PROTEIN BY ANTI-SULFATIDE ANTIBODY AND INTERFERON-? IN MYELIN FROM GLIA MATURATION FACTOR-DEFICIENT MICE

Risk factors for thrombosis in lupus patients.

In this study we show the effect of anti-sulfatide (RmAb) antibodies and inflammatory cytokines, TNF-α and IFN-γ in inducing myelin basic protein (MBP) degradation in myelin...Full Text Available

2007-06-01

7

Identification of circulating neuropilin-1 and dose-dependent elevation following anti-neuropilin-1 antibody administration

Lupus anticoagulant, concentrations of anticardiolipin antibodies, antithrombin III, plasminogen, (free) protein S, protein C, prothrombin, platelet counts, and bleeding times were determined in 74...Full Text Available

1989-11-01

8

Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression of forkhead transcription factor and atrophy gene transcription

Neuropilin-1 (NRP1) acts as a co-receptor for class 3 semaphorins and vascular endothelial growth factor and is an attractive angiogenesis target for cancer therapy. In addition to the transmembrane...Full Text Available

2009-07-01

9

FoxO Transcription Factors in Brain: Regulation and Behavioral Manifestation

Cellular hypertrophy is regulated by coordinated pro- and antigrowth machineries. Foxo transcription factors initiate an atrophy-related gene program to counter hypertrophic growth. This study was designed...Full Text Available

2008-09-01

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Polyunsaturated Fatty Acids Selectively Suppress Sterol Regulatory Element-binding Protein-1 through Proteolytic Processing and Autoloop Regulatory Circuit*

BackgroundThe mammalian FoxO transcription factors function to regulate diverse physiological processes. Emerging evidence that both BDNF and lithium suppress FoxO...Full Text Available

2009-01-15

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Antihuman growth hormone (GH) antibodies cross-react with the GH-like factor from plerocercoids of the tapeworm Spirometra mansonoides.

Sterol regulatory element-binding protein (SREBP)-1 is a key transcription factor for the regulation of lipogenic enzyme genes in the liver. Polyunsaturated fatty acids (PUFA) selectively suppress hepatic...Full Text Available

2010-04-09

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Validation of immune function testing during a 4-week oral toxicity study with FK506.

A factor produced by the plerocercoid stage of S. mansonoides mimics some, but not all, of the actions reported for hGH. The biological actions of plerocercoid growth factor (PGF) suggest structural similarity to human GH (hGH). Plerocercoid membranes were solubilized, and PGF was purified more than 1000-fold by hGH receptor affinity chromatography. The ability of purified PGF to displace [125I]hGH from monoclonal antibodies specific for four distinct nonoverlapping antigenic determinants of hGH and from an anti-hGH polyclonal antibody was tested in liquid phase RIA. All of the hGH antibodies cross-reacted with PGF, with potencies ranging from more than 60% to less than 1% that of the hGH standard. Of the four major epitopes of hGH defined by the monoclonal antibodies used in this study, only one is not represented to a significant extent in PGF. The epitope of ...

1987-11-01

13

Generation and Characterization of Monoclonal Antibodies to Human Keratinocyte Growth Factor Receptor

Assessment of the immune system's capability to respond to antigens with the generation of specific antibodies, whilst under the influence of a test article, is required in toxicity tests according to the European guideline for repeated dose toxicity testing of medicinal products. The purpose of this study in rats was to validate methodology for the determination of Keyhole Limpet Haemocyanin (KLH)-specific antibodies under the influence of an immunologically active compound. The immunosuppressant FK506, commercially available as Prograf, was administered orally (gavage) to five rats per sex per group at dosages of 0.5mg/kg per day or 3mg/kg per day, for a period of 4 weeks. On days 14 and 22, KLH was administered subcutaneously, with an adjuvant (AluGel), to the two treated groups and a control (i.e. without FK506 treatment) approximately 1h following administration of FK506. Terminal investigations included haematology parameters, titration ...

2004-04-01

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British Library Electronic Table of Contents (United Kingdom)

Keratinocyte growth factor receptor (KGFR) and fibroblast growth factor receptor (FGFR) 2c share identical amino acid sequences, except for a 46-amino acid domain in the extracellular region. Monoclonal antibodies (MAbs) specific to KGFR have not been reported nor are commercially available. In this study, we generated murine MAbs specific to KGFR in non-obese diabetic (NOD) mice using a modified Repeated Immunizations at Multiple Sites (RIMMS) technology. Stable cell lines expressing the full-length human KGFR or FGFR2c were produced to facilitate the identification of KGFR-specific MAbs. Following the initial screening of hybridoma clones with a fluorescence-based, confocal cell detection method and ELISA, KGFR-specific MAbs were selected and confirmed by flow cytometry and Western blot ...

2006-01-01

15

Commercially Available Antibodies to Human Tumour Necrosis Factor-? Tested for Cross-Reactivity with Ovine and Bovine Tumour Necrosis Factor-? using Flow Cytometric Assays

The protozoan parasite Theileria annulata alters the differentiation state of the infected macrophage and suppresses musculoaponeurotic fibrosarcoma oncogene (MAF) transcription factors

A thorough understanding of the immune system, including the role of different cytokines, during inflammatory diseases in ruminants could lead to the development of new diagnostic methods and treatments....Full Text Available

2004-01-01

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The Regulation of Aging and Longevity

AbstractThe tick-borne protozoan parasite Theileria annulata causes a debilitating disease of cattle called Tropical Theileriosis. The parasite predominantly invades...Full Text Available

2009-08-01

17

SirT3 suppresses hypoxia inducible factor 1? and tumor growth by inhibiting mitochondrial ROS production

p53 plays a critical role in tumor suppression. As a transcription factor, in response to stress signals, p53 regulates its target genes and initiates stress responses, including cell cycle arrest,...Full Text Available

2011-04-01

18

Amorphigenin inhibits Osteoclast differentiation by suppressing c-Fos and nuclear factor of activated T cells

It has become increasing clear that alterations in cellular metabolism have a key role in the generation and maintenance of cancer. Some of the metabolic changes can be attributed to the activation...Full Text Available

2011-06-30

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Establishment of functional primary cultures of heart cells from the clam Ruditapes decussatus

Among the several rotenoids, amorphigenin is isolated from the leaves of Amopha Fruticosa and it is known that has anti-proliferative effects and anti-cnacer effects in many cell types....Full Text Available

2010-12-01

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British Library Electronic Table of Contents (United Kingdom)

Heart cells from the clam Ruditapes decussatus were routinely cultured with a high level of reproducibility in sea water based medium. Three cell types attached to the plastic after 2?days and could be maintained in vitro for at least 1?month: epithelial-like cells, round cells and fibroblastic cells. Fibroblastic cells were identified as functional cardiomyocytes due to their spontaneous beating, their ultrastructural characteristics and their reactivity with antibodies against sarcomeric ?-actinin, sarcomeric tropomyosin, myosin and troponin T-C. Patch clamp measurements allowed the identification of ionic currents characteristic of cardiomyocytes: a delayed potassium current (I K?slow) strongly suppressed (95%) by tetraethylammonium (1?mM), a fast inactivating potassium current (I K?fas...

2011-01-01

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Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells

Regression of rat mammary tumors associated with suppressed growth hormone.

The purpose of the present study was to examine the in vitro effects of low-dose cadmium (Cd) on developing cortical cells. The cortical cells removed from fetuses (embryonic day 15) were treated with 10 nM of Cd for 24 h. The effects of Cd on dendritic and synaptic development were immunocytochemically observed with anti-microtubule associated protein-2 (MAP2) and anti-synapsin I antibodies, respectively. Administration of Cd suppressed dendritic as well as synaptic development at 10 nM. By two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometric (LC/MS/MS) analysis, we identified three proteins with different expression after Cd-treatment; dihydropyrimidinase-related protein 2 (DRP-2/CRMP-2), 14-3-3-epsillon and calmodulin (CaM). Though the number of identified proteins was small, these proteins are known to be involved in neuronal development. The present study demonstrated the morphological effects as well as ...

2008-11-20

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Cumulative effect of X-ray radiation and inflammatory reaction on the circadian rhythm of tyrosine aminotransferase in the liver of mice

Serum growth hormone (GH) was suppressed in female rats bearing mammary tumors induced by 7, 12, dimethylbenz(a)anthracene (DMBA) or N-nitrosomethylurea(NMU). Serum GH was suppressed due to treatment with a human GH analog produced by the plerocercoid stage of the tapeworm Spirometra mansonoides. Rats treated with plerocercoid growth factor (PGF) via plerocercoid infection had accelerated growth rates despite marked reductions in GH levels. Approximately two-thirds of the mammary tumors induced by either DMBA or NMU regressed during three weeks of exposure to PGF while most of the control tumors continued to grow. The data support an important regulatory role for GH in growth of mammary tumors in rats. PMID:3019224

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The EGFR antibody cetuximab induces autophagy in cancer cells by downregulating HIF-1? and Bcl-2 and activating the beclin-1/hVps34 complex

The activity of tyrosine aminotransferase (TAT) in the liver of mice subjected simultaneously to ionizing radiation and to local inflammatory process showed in the first 24 hours of the experiment statistically significant changes resulting from summation of the harmful effects of both factors. In the second 24-hour period an evident tendency was observed for return of a normal circadian rhythm of the enzyme. This points to presence of an endogenous mechanism of suppression of the manifestations of significant desynchronization of the circadian rhythm. The paper contains also a trial of mathematical analysis of changes in TAT activity in the circadian rhythm, a description and analysis or the suppression process, qualitative and quantitative determination of the phenomenon of cumulation of the effects of powerful stress factors acting on the animal organism. The obtained results were compared with the ...

1980-01-01

24

Diversion of the Immune Response to Neisseria gonorrhoeae from Th17 to Th1/Th2 by Treatment with Anti-Transforming Growth Factor ? Antibody Generates Immunological Memory and Protective Immunity

Autophagy is a regulated catabolic process triggered in cells deprived of nutrients or growth factors that govern nutrient uptake. Here we report that autophagy is induced by cetuximab, a therapeutic...Full Text Available

2010-07-15

25

Principles of antibody therapy.

ABSTRACTThe immune response to Neisseria gonorrhoeae is poorly understood, but its extensive antigenic variability and resistance to complement are thought to allow it...Full Text Available

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Radiochemistry of lead-203 for radiolabeling antibody conjugates

The success of monoclonal antibodies in clinical practice is dependent on good design. Finding a suitable target is the most important part as other properties of the antibody can be altered by genetic...Full Text Available

1992-12-05

27

Radiochemistry of lead-203 for radiolabeling antibody conjugates

Short note.

1991-01-01

28

Immunoglobulins in myasthenia gravis. Kinetic properties of the acetylcholine-receptor antibody studied during lymph drainage.

Brief item.

29

Development of sup 203 Pb as a new label for monoclonal antibodies

A specific immunoglobulin, the receptor antibody, can be found in most patients with myasthenia gravis. In order to study the kinetic properties of this antibody, serial determinations of receptor antibody,...Full Text Available

1978-10-01

30

Suppression of receptors for prolactin and estrogen in rat liver due to treatment with the growth hormone analogue produced by the tapeworm Spirometra mansonoides.

Brief item.

1989-01-01

31

Localization of an anti-tumour monoclonal antibody in human tumour xenografts: kinetic and quantitative studies with the 791T/36 antibody

Somatogenic hormones play an important role in regulation of receptors for prolactin (PRL) and estrogen. Plerocercoids of the tapeworm, S. mansonoides produce a factor which mimics some, but not all of the actions reported for GH. Intact female rats were subjected to a constant infusion of plerocercoid growth factor (PGF) via a subcutaneous infection for two weeks to determine if PGF influences receptors for PRL, GH or estradiol. The rate of weight gain in the PGF-treated rats was accelerated in spite of a marked reduction in serum GH. Partially-purified PGF specifically displaced [125I]hGH from rat liver receptors but microsomes prepared from rats treated with PGF specifically bound significantly less [125I]hGH than microsomes from control rats. The reduction in [125I]hGH binding was not due to occupancy or to a change in affinity but to a suppression in receptor concentration. Scatchard analysis of [3H]estradiol binding ...

1986-01-01

32

miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NF?B1

Studies have been performed with an anti-osteogenic sarcoma monoclonal antibody 791T/36 and human tumour xenografts to examine some of the quantitative and kinetic aspects of tumour localization. The influence of variations in tumour size and site and antibody dose on the extent and rate of specific antibody localization have been examined, together with the site of intratumoural deposition of the antibody. (UK).

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Suppression of the transverse scattering amplitude in /sup 207/Pb

The activation of nuclear factor-kappa B1 (NFκB1) in cancer cells may confer resistance to ionizing radiation (IR). To enhance the therapeutic efficiency of IR in lung cancer, we screened for...Full Text Available

2011-05-31

34

(?) Epigallocatechin gallate suppresses the differentiation of 3T3-L1 preadipocytes through transcription factors FoxO1 and SREBP1c

Transverse form factors have been extracted for the low-lying neutron hole (particle) states of /sup 207/Pb from inelastic-electron-scattering data. A systematic, multipolarity-and momentum-transfer-independent quenching of approx.55% in the transverse amplitude is observed when compared with single particle predictions for both electric and magnetic transitions. The magnitude of the observed effect is not readily explained by our present theoretical understanding of this nucleus.

1980-07-14

35

Novel method for differentiation between Trastuzumab and host adaptive response.

Tea catechin is one of the compounds that are closely related to obesity and insulin sensitivity. In order to determine the effect of catechin on adipocyte differentiation, we treated 3T3-L1 preadipocytes...Full Text Available

2010-06-01

36

Development of an assay for a biomarker of pregnancy and early fetal loss

Humoral immune response to human epidermal growth factor receptor 2 (HER-2/neu or ErbB-2) has been detected in sera of breast cancer patients and shown to be an appropriate prognostic marker (Taylor et al., 2007). However, since Trastuzumab (Herceptin) is a widely used monoclonal antibody as cancer therapy agent for tumors over-expressing HER-2, there is a need for an efficient way to detect host-generated antibodies against HER-2 without the confounding effect of Herceptin. Here we describe a screening method developed to decipher between host antibodies against HER-2 and that of Herceptin. By producing a series of truncation mutants within the epitope of Herceptin, we were able to inhibit this binding. We demonstrated also that by a three amino acid substitution (PPF?SSS) we were able to abrogate Herceptin binding while generating a highly conserved HER-2 extracellular domain (ECD). By producing a ...

2011-06-08

37

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation

Human chorionic gonadotropin (hCG) is a glycoprotein hormone, secreted by the syncytiotrophoblast cells of the fertilized ovum, that enters the maternal circulation at the time of endometrial implantation. It is composed of two nonidentical subunits; ..cap alpha.. and ..beta.., with molecular weights of 14 kD and 23 kD, respectively. Human chorionic gonadotropin binds to the same receptor as hLH and displays the same biological response, namely, to stimulate the declining function of the corpus luteum to produce progestins and estrogen late in the menstrual cycle. The differences in the structures of hCG and hLH have been exploited to develop antibodies that can measure hCG specifically in the presence of hLH. Two-site antibody binding assays have been developed, based on a surface immunological concept of hCG epitopes, that involve four distinct regions to which antibodies against hCG can bind simultaneously. ...

1987-10-01

38

British Library Electronic Table of Contents (United Kingdom)

Multicellular tumor spheroids (MCTS) are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 (HPV16). Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24?h of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western bl...

2008-01-01

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A novel small-molecule inhibitor of NF-#kappa#B signaling

Extracranial-intracranial bypass surgery utilizing homologous arterial grafts irradiated with high voltage cathode rays. Experimental study and clinical application

The inducible transcription factor NF-#kappa#B regulates divergent signaling pathways including inflammatory response and cancer development. Selective inhibitors for NF-#kappa#B signaling are potentially useful for treatment of inflammation and cancer. NF-#kappa#B is canonically activated by preferential disposal of its inhibitory protein; I#kappa#B, which suppresses the nuclear translocation of NF-#kappa#B. I#kappa#B#alpha# (a major member of I#kappa#B family proteins) is phosphorylated with an I#kappa#B kinase (IKK) and subsequently polyubiquitylated by SCF"#beta#"T"r"C"P"1 ubiquitin-ligase in the presence of E1 and E2 prior to proteasomal degradation. Here, we describe a novel inhibitor termed GS143, which suppressed I#kappa#B#alpha# ubiquitylation, but not I#kappa#B#alpha# phosphorylation, MDM2-directed p53 ubiquitylation, and proteasome activity in vitro. GS143 markedly suppressed the destruction ...

2008-04-18

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Extracranial-intracranial bypass surgery utilizing homologous arterial grafts irradiated with high voltage cathode rays

Homologous and heterologous arterial segments were implanted in Fisher rats subcutaneously for the purpose of examining the antibody titer of the recipients' serum after implantation by means of the immune-adherence hemagglutination method. The antibody titer after implantation both of homologous and heterologous grafts decreased to 1/8 by 2.0 million (M) rads irradiation of high voltage cathode rays. The results suggested that high voltage cathode ray irradiation was not enough for heterologous graft to suppress its tissue reaction. Homografts taken from dogs 3 or 6 hours after sacrifice were irradiated with 2.0 M rads and transplanted in canine carotid artery using the technic of end-to-end anastomosis. Angiograms 6 months after operation revealed excellent patency rate in all the grafts of 28 dogs. furthermore, findings of the grafts from 1 week to 5 years after operation on scanning and transmission electron ...

1982-06-01

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Monoclonal antibodies: new agents for cancer detection and targeted therapy

Homologous and heterologous arterial segments were implanted in Fisher rats subcutaneously for the purpose of examining the antibody titer of the recipients' serum after implantation by means of the immune-adherence hemagglutination method. The antibody titer after implantation both of homologous and heterologous grafts decreased to 1/8 by 2.0 million (M) rads irradiation of high voltage cathode rays. The results suggested that high voltage cathode ray irradiation was not enough for heterologous graft to suppress its tissue reaction. Homografts taken from dogs 3 or 6 hours after sacrifice were irradiated with 2.0 M rads and transplanted in canine carotid artery using the technic of end-to-end anastomosis. Angiograms 6 months after operation revealed excellent patency rate in all the grafts of 28 dogs. furthermore, findings of the grafts from 1 week to 5 years after operation on scanning and transmission electron ...

1982-01-01

42

Extracellular Protein Disulfide Isomerase Regulates Feedback Activation of Platelet Thrombin Generation Via Modulation of Coagulation Factor Binding.

Antibodies directed against markers on cancer cells are gaining in importance for the purpose of targeting diagnostic and therapeutic agents. In the past, this approach has had very limited success principally because the classical methods for producing antibodies from blood serum of animals immunized with cancer cells or extracts were unsatisfactory. The situation has changed dramatically since 1975 following the design of procedures for 'immortalizing' antibody-producing cells (lymphocytes) by fusing them with cultured myeloma cells to form hybridomas which continuously secrete antibodies. Since these hybridomas produce antibodies coded for by a single antibody-producing cell, the antibodies are called monoclonal. Building on these advances in biomedical research, it is now possible to reproducibly manufacture monoclonal ...

43

Anti-nuclear antibody positivity and the use of certolizumab in inflammatory bowel disease patients who have had arthralgias or lupus-like reactions from infliximab or adalimumab

Background: Protein disulfide isomerase (PDI) controls platelet integrin function, tissue-factor (TF) activation, and concentrates at fibrin and thrombus formation sites of vascular injury. Objective: We investigated involvement of surface thiol isomerases and especially PDI, in thrombin-mediated thrombin amplification on human platelets. Methods/Results: Using a new developed thrombin-dependent platelet thrombin generation assay we observed that the feedback activation of thrombin generation on the platelet surface does not depend on TF, as anti-TF antibodies inhibiting TF-induced thrombin formation in platelet-depleted plasma had no effect compared to vehicle-treated controls. Feedback activation of thrombin generation in the presence of platelets was significantly diminished by membrane impermeant thiol blockers or by the thiol isomerase-inhibitors bacitracin and anti-PDI antibody RL90, respectively. Platelet thrombin ...

2011-09-19

44

British Library Electronic Table of Contents (United Kingdom)

OBJECTIVE:- Tumor necrosis factor (TNF) inhibitors can be used to treat inflammatory bowel disease (IBD) but may lead to anti-nuclear antibody (ANA) positivity and lupus-like reactions. Because of its unique structure, certolizumab has lower rates of these complications. We sought to investigate whether patients who have had lupus-like reactions to infliximab or adalimumab would be able to tolerate certolizumab. METHODS:- We performed a retrospective analysis on the 23 patients at the Roberts Inflammatory Bowel Disease Center who received certolizumab for the treatment of Crohn's disease from March 2008 to June 2009. We identified 6 patients who were switched to this drug because of lupus-like reactions from prior anti-TNF therapy and had documented ANA after the reaction and prior to cert...

2011-01-01

45

Erythroid Differentiation Regulator 1, an Interleukin 18-Regulated Gene, Acts as a Metastasis Suppressor in Melanoma

British Library Electronic Table of Contents (United Kingdom)

Erythroid differentiation regulator (Erdr1) was first discovered in mouse leukemia cell lines and functions as a stress-related survival factor. This study investigated whether Erdr1 regulates murine melanoma progression, as well as the mechanism involved in Erdr1-regulated metastasis. The expression of Erdr1 is negatively correlated with IL-18 expression, which has a pro-cancer effect in melanoma. To study the role of Erdr1 as an anti-cancer factor, cell migration, invasion, and proliferation were measured. Erdr1 overexpression markedly inhibited the level of cell migration, invasion, and proliferation in B16F10 cells in vitro. In addition, Erdr1 overexpression significantly suppressed melanoma lung colonization, metastasis, and tumor growth in vivo. To identify the factors involved in Er...

2011-01-01

46

Solid-Phase Radioimmunoassay of Total and Influenza-Specific Immunoglobulin G

M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.

An antigen-antibody system of polystyrene tubes coated with immunoglobulin antibody was used for quantitating immunoglobulins. A similar radioimmunoassay method was adapted for a viral antigen-antibody...Full Text Available

1972-02-01

47

Comparative Inhibitory Effects of Antigen and Antibody in the Staphylococcal Enterotoxin Solid-Phase Radioimmunoassay System

A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three...Full Text Available

1989-09-01

48

Antibody avidity in swine lymphocyte antigen-defined miniature pigs.

A solid-phase radioimmunoassay employing ¹²⁵I-labeled enterotoxins and polystyrene tubes coated with specific antibody has been developed for assaying the relative concentrations of antibodies...Full Text Available

1975-06-01

49

Anti-albumin antibodies in chronic liver diseases: diagnostic significance of these antibodies in patients with conventional or immunosuppressive therapy.

Antibody avidity to hen egg white lysozyme (HEWL) was measured by thiocyanate ion elution enzyme-linked immunosorbent assay (ELISA) in swine lymphocyte antigen (SLA) defined miniature pigs. Serum antibody...Full Text Available

1992-10-01

50

Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma

The two categories of anti-albumin antibodies (AAA), namely precipitins (AA-P) and agglutinins (AA-Aggl), were investigated in 260 patients with morphologically diagnosed chronic liver diseases (CLD)....Full Text Available

1981-02-01

51

Amastigote stage-specific monoclonal antibodies against Leishmania major.

SummaryMonoclonal antibodies are standard therapeutics for several cancers including the anti-CD20 antibody rituximab for B cell non-Hodgkin lymphoma (NHL). Rituximab and other...Full Text Available

2010-09-03

52

Roles for Rat Hepatocyte Malignant Transforming Factor (HMTF) in Late Stage of Hepatocarcinogenesis.

Monoclonal antibodies were produced against gamma-irradiated amastigotes of Leishmania major. Five antibodies (T16 through T20) were selected which reacted in enzyme-linked immunoassays with the intracellular...Full Text Available

1989-12-01

53

Neutron diffraction study of 5f itinerant antiferromagnet UPtGa{sub 5} and UNiGa{sub 5}

In a previous study, to identify genes of importance for hepatocellular carcinogenesis, and especially for processes involved in malignant transformation, the authors investigated differences in gene expression between adenomas and carcinomas by DNA microarray. In the present study, the authors investigated AW434047, one of the sequences that was upregulated in carcinomas. The investigation led to the identification of a novel gene, which the authors named hepatocyte malignant transforming factor (HMTF), of unknown function whose expression was increased in hepatocellular carcinomas. Northern blot and in situ hybridization also demonstrated high levels of HMTF in rat hepatocellular carcinoma (HCC) cell lines, lymphocytes in the spleen, colon mucosal epithelia, spermatocytes, and granule cells of the hippocampus. Reduction of HMTF by RNA interference (RNAi) in N1 cells, an HCC cell line, caused suppression of cell proliferation, invasion, and ...

2011-09-20

54

Encapsulated magnetite particles for biomedical application

Magneto-striction and magnetic form factors in 5f itinerant antiferromagnets UNiGa{sub 5} and UPtGa{sub 5} are studied by means of neutron scattering. Remarkable magneto-striction was observed around T{sub N}, indicating large spin-orbit coupling in the itinerant system. The orbital magnetic moment is found to be strongly suppressed due to the hybridization of uranium 5f with Ga-4p electron.

2003-05-01

55

A model for the calculation of vent clearing transients in pressure suppression systems

The process of miniemulsification allows the generation of small, homogeneous, and stable droplets containing monomer or polymer precursors and magnetite which are then transferred by polymer reactions to the final polymer latexes, keeping their particular identity without serious exchange kinetics involved. It is shown that the miniemulsion process can excellently be used for the formulation of polymer-coated magnetic nanoparticles which can further be used for biomedical applications. The use of high shear, appropriate surfactants, and the addition of a hydrophobe in order to suppress the influence of Ostwald ripening are key factors for the formation of the small and stable droplets in miniemulsion and will be discussed. Two different approaches based on miniemulsion processes for the encapsulation of magnetite into polymer particles will be presented in detail.

2003-04-23

56

Susceptibility to invasive bacterial infections in children with sickle cell disease

For the layout of a pressure suppression system of a light water cooled reactor (boiling water reactor) it is important to know the time dependent behavior of the vent clearing transient after a loss-of-coolant accident for two main reasons: time of the end of the vent clearing transient influences strongly the pressure and temperature maxima in the drywell and wetwell. Time-dependent behavior of the vent clearing transient influences pressure loads in the condensation pool of the wetwell and therefore pressure induced stresses to the structure. The time-dependent behavior of the water masses in the vent pipes and wetwell are described by the basic equations for a nonstationary incompressible friction flow: momentum equation, continuity equation and a correlation for the variation of the state of the gas volume in the wetwell above the water level. After many algebraic operations and integrations along the flow path, a single ordinary nonlinear differential ...

1975-09-01

57

British Library Electronic Table of Contents (United Kingdom)

Individuals with sickle cell disease (SCD) demonstrate an increased susceptibility to invasive bacterial infections (IBI). The most common organisms causing IBI are Streptococcus pneumoniae, nontyphi Salmonella species and Haemophilus influenzae type b (Hib). IBI are the most common causes of death in children below 5 years of age with SCD. Increased susceptibility to IBI is because of several factors including dysfunctional antibody production and opsonophagocytosis as well as defective splenic clearance. Early diagnosis of Hib and pneumococcal infections combined with antibiotic prophylaxis and immunization programs, could lead to significant improvements in mortality, especially in Africa. Pediatr Blood Cancer. 2010;55:401-406. Copyright 2010 Wiley-Liss, Inc.

2010-01-01

58

Serological Reactivity to Borrelia burgdorferi Sensu Lato in Dogs and Horses from Distinct Areas in Romania

British Library Electronic Table of Contents (United Kingdom)

Abstract Lyme disease is a perfect model of the complex relationship between host, vector, and the vector-borne bacteria. Both dogs and horses in Romania are exposed to infection. The aim of the present study was to assess the seroreactivity against Borrelia burgdorferi sensu lato in dogs and horses from different regions of Romania. 276 samples from dogs and 260 samples from horses located in different regions of Romania were analyzed by ELISA and IFA, respectively. The effect of several factors potentially affecting seroreactivity (location, age, gender, occupation, and vector exposition risk) was evaluated using Fisher's exact test (R 2.12.0). The overall prevalence of anti-Borrelia antibodies was 6.52% (18/276) in dogs, with a significantly higher positivity (46.15%, 6/13, p=0.0005) re...

2011-01-01

59

Radioisotopes for radioimmunodetection (RAID) and radioimmunotherapy (RAIT)---current and new perspectives

Experimental parameters differentially affect the humoral response of the cholera-toxin-based murine model of food allergy

In this paper the availability and properties of radioisotopes for both radioimmunodiagnosis (RAID) and radioimmunotherapy (RAIT) are discussed. Examples are provided for radioisotopes available via direct production in nuclear reactors and accelerators or as daughters obtained from radionuclide generator systems whose parents are either reactor or accelerator produced. Important factors which must be considered for the use of a particular radioisotope include availability, the physical half-life and decay properties, and chemical versatility for protein attachment. Although both direct'' and indirect'' methods are available for attachment of radioisotopes to antibodies, this broad field of research is not reviewed in detail. Practical issues related to the availability and use of a variety of radionuclides are described. 47 refs., 5 tabs.

1991-01-01

60

Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans.

Background: Recent studies have developed a murine model of IgE-mediated food allergy based on oral coadministration of antigen and cholera toxin (CT) to establish a maximal response for studying immunopathogenic mechanisms and immunotherapeutic strategies. However, for studying subtle immunomodulating factors or factors effective during response initiation, this maximal response-based model is less suitable due to a lack of sensitivity. Therefore, in attempts to identify essential parameters to fine-tune the immune response towards a submaximal level, potentially more sensitive, we were interested in characterizing the individual effects of the parameters in the CT-based model: CT dose, antigen type and dose, and number of immunizations. Methods: BALB/c mice were orally sensitized weekly for 3 or 7 weeks with graded doses of CT and various food antigens (soy-trypsin inhibitor, ovalbumin or ovomucoid). Antigen-specific lgG1, IgG2a, IgA and IgE ...

2003-01-01

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61

Local and systemic antibody response to bovine respiratory syncytial virus infection and reinfection in calves with and without maternal antibodies.

Udgivelsesdato: 1985-Feb

1985-01-01

62

Human anti-rhesus D IgG1 antibody produced in transgenic plants.

Enzyme-linked immunosorbent assays for the detection of immunoglobulin M (IgM), IgA, IgG1, and IgG2 antibodies against bovine respiratory syncytial virus (BRSV) were used to measure antibody responses...Full Text Available

1987-06-01

63

Heterophyle antibodies causing false positive radio-immunoassay results a case report

Udgivelsesdato: 2002-Apr

2002-01-01

64

Distribution of Borrelia burgdorferi specific antibody among patients with juvenile rheumatoid arthritis in Korea.

A case report of falsely elevated serum hormone values measured by radio-immunoassay (RIA) is described. The radio-immunoassays concerned have a first antibody raised in rabbits and mostly a separation technique based on a second antibody-solid phase system. The presence of heterophyle (anti-rabbit) antibodies in patients' serum is proved.

65

An immunochemical approach to the study of DNA damage and repair

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1993-12-01

66

A Monoclonal Antibody That Specifically Binds Chitosan In Vitro and In Situ on Fungal Cell Walls

The overall objective of this project is to produce antibodies to unique modified DNA bases and develop immunochemical assays to quantitate these lesions in damaged DNA. During this past year we have developed an antibody and chemical test to quantitate a basic sites in DNA and produced antibodies to the 8-oxopurines. This report discusses the detection of a basic sites in DNA and the preparation of antibodies to 8-hydroxyadenine and 8-hydroxyguanine.

1990-11-14

67

Preparation of monoclonal antibodies labelled by astatine

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2010-08-01

68

Bevacizumab as a treatment option for radiation-induced cerebral necrosis

Astatine conversed into cationic form is shown to form stable complex with diethylenetriaminpentacetic acid. Due to this complex astatine joins RN_2 type monoclonal antibodies. More favorable conditions to prepare astatine labelled antibodies are found. Chromatographical analysis and electromigration experiments have shown that astatine is strongly retained in in-vitro biomolecule. Astatine did not escape from the labelled antibodies even in case of urea effect on them. Immune activity of astatine labelled antibodies remained similar in 20 h. 28 refs., 4 figs.

69

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation.

Radiation necrosis of normal CNS tissue represents one of the main risk factors of brain irradiation, occurring more frequently and earlier at higher total doses and higher doses per fraction. At present, it is believed that the necrosis results due to increasing capillary permeability caused by cytokine release leading to extracellular edema. This process is sustained by endothelial dysfunction, tissue hypoxia, and subsequent necrosis. Consequently, blocking the vascular endothelial growth factor (VEGF) at an early stage could be an option to reduce the development of radiation necrosis by decreasing the vascular permeability. This might help to reverse the pathological mechanisms, improve the symptoms and prevent further progression. A patient with radiation-induced necrosis was treated with an anti-VEGF antibody (bevacizumab), in whom neurologic signs and symptoms improved in accordance with a decrease in T1-weighted ...

2011-02-01

70

Inhibitor of DNA synthesis is present in normal chicken serum

Multicellular tumor spheroids (MCTS) are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 (HPV16). Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24 h of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western blot for E-cadherin detected upregulation of the authentic 120 kDa band from MCTS of CaSki cells as well as a shorter 100 kDa band. Addition of EGF, whose ...

2008-01-01

71

3D time-of-flight MR angiography of the intracranial vessels: optimization of the technique with water excitation, parallel acquisition, eight-channel phased-array head coil and low-dose contrast administration

The authors have found that heat-inactivated serum (57/sup 0/C for 1 hour) from normal chickens reduces the proliferation of mitogen-stimulated chicken and murine splenocytes as well as some transformed mammalian lymphoblastoid cell lines. Greater than a 50% reduction in /sup 3/H-thymidine incorporation was observed when concanavalin A (Con A)-activated chicken splenocytes that were cultured in the presence of 10% autologous or heterologous serum were compared to mitogen-stimulated cells cultured in the absence of serum. Normal chicken serum (10%) also caused greater than 95% suppression of /sup 3/H-thymidine incorporation by bovine (EBL-1 and BL-3) and gibbon ape (MLA 144) transformed lymphoblastoid cell lines. The only cell line tested that was not inhibited by chicken serum was an IL-2-dependent, murine cell line. Chicken serum also inhibited both /sup 3/H-thymidine incorporation and IL-2 synthesis by Con A-activated murine splenocytes. ...

1986-03-05

72

Western Blot Analysis of Cereal Grain Prolamins Using an Antibody to Carboxyl-Linked Indoleacetic Acid

The aim of this study is three folds: to compare the eight-channel phased-array and standard circularly polarized (CP) head coils in visualiazation of the intracranial vessels, to compare the three-dimentional (3D) time-of-flight (TOF) MR angiography (MRA) techniques, and to define the effects of parallel imaging in 3D TOF MRA. Fifteen healthy volunteers underwent 3D TOF MRA of the intracranial vessels using eight-channel phased-array and CP standard head coils. The following MRA techniques were obtained on each volunteer: (1) conventional 3D TOF MRA with magnetization transfer; (2) 3D TOF MRA with water excitation for background suppression; and (3) low-dose (0.5 ml) gadolinium-enhanced 3D TOF MRA with water excitation. Results are demonstrating that water excitation is a valuable background suppression technique, especially when applied with an eight-channel phased-array head coil. For central and proximal portions of the intracranial ...

2004-11-01

73

Targeting Prostate Cancer Cells In Vivo Using a Rapidly Internalizing Novel Human Single-Chain Antibody Fragment

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor,...Full Text Available

1991-08-01

74

Staphylococcal Enterotoxin B: Solid-Phase Radioimmunoassay

Human antibodies targeting prostate cancer cell surface epitopes may be useful for imaging and therapy. The objective of this study was to evaluate the tumor targeting of an internalizing human...Full Text Available

2010-03-01

75

Relationship between Legionella spp. and antibody titres at a therapeutic thermal spa in Portugal.

An immunoassay employing ¹²⁵I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated...Full Text Available

1971-11-01

76

Quantitation of Antibody to Non-Hemagglutinating Viruses by Single Radial Hemolysis: Serological Test for Human Coronaviruses

The presence of Legionella spp. in the water of a Portuguese spa was ascertained during the spa season, between May and November. Simultaneously the prevalence of anti-legionella antibodies in people...Full Text Available

1995-08-01

77

Proteome-wide Anti-HCV and Anti-HIV Antibody Profiling for Predicting and Monitoring Response to HCV Treatment in HIV Co-infected Patients

A single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. With the human coronaviruses as models, this test utilizes the binding properties...Full Text Available

1977-06-01

78

Observations on the use of solid-phase-coupled antibodies in the radioimmunoassay of human placental lactogen

We quantified antibody responses to the HCV proteome that are associated with sustained virologic response (SVR) in HIV/HCV co-infected patients treated with pegylated interferon and ribavirin....Full Text Available

2010-09-15

79

Novel Approach for Differential Diagnosis of HIV Infections in the Face of Vaccine-Generated Antibodies

A detailed comparative assessment was made of the use of solid-phase-coupled antibodies in radioimmunoassay, by using an assay for human placental lactogen as a model system. The major advantages of...Full Text Available

1974-03-01

80

Microglial Fc Receptors Mediate Physiological Changes Resulting From Antibody Cross-Linking of Myelin Oligodendrocyte Glycoprotein

SummaryBecause increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine-from virus-induced antibodies. Most of the currently...Full Text Available

2006-11-01

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81

In vitro antigen-induced antibody responses to hepatitis B surface antigen in man. Kinetic and cellular requirements.

Antibodies to myelin oligodendrocyte glycoprotein (MOG) have been implicated in Multiple Sclerosis demyelination through activation of complement and/or macrophage-effector processes. We presented...Full Text Available

2008-05-30

82

Evaluation of Multiplexed Fluorescent Microsphere Immunoassay for Detection of Autoantibodies to Nuclear Antigens

In this report we define the parameters of the human immune response after immunization with hepatitis B vaccine. 2 wk after booster immunization, there is significant spontaneous secretion of antibody...Full Text Available

1984-10-01

83

Dried blood spots as a source of anti-malarial antibodies for epidemiological studies

Antibodies to extractable nuclear antigens (ENA) are found in a variety of collagen vascular diseases. Determining the individual specificities of these antibodies is extremely useful in establishing...Full Text Available

2004-11-01

84

Development and Application of Pathovar-Specific Monoclonal Antibodies That Recognize the Lipopolysaccharide O Antigen and the Type IV Fimbriae of Xanthomonas hyacinthi

BackgroundBlood spots collected onto filter paper are an established and convenient source of antibodies for serological diagnosis and epidemiological surveys. Although recommendations...Full Text Available

85

Detection of rotavirus by serological trapping on antibody-coated electron microscope grids.

The objective of this study was to develop a specific immunological diagnostic assay for yellow disease in hyacinths, using monoclonal antibodies (MAbs). Mice were immunized with a crude cell wall preparation...Full Text Available

1999-09-01

86

Cryptosporidium parvum (Apicomplexa: Cryptosporidiidae) oocyst and sporozoite antigens recognized by bovine colostral antibodies.

A serological trapping technique for detecting rotaviruses is described which involves coating electron microscope grids with protein A and specific rotavirus antiserum. The presence of a layer of antibodies...Full Text Available

1980-07-01

87

Clinical Significance of the Detection of Antinuclear Antibodies in Patients with Acute Hepatitis A

Colostral whey from seven hyperimmunized and two control cows (hyperimmune bovine colostrum) was examined by Western immunoblotting for the presence of antibody against oocysts and sporozoites of Cryptosporidium...Full Text Available

1990-09-01

88

Circulating antibody to myelin basic protein in relapsing-remitting multiple sclerosis? A comparative group and sequential study by radioimmunoassay.

Background/AimsThe findings of several recent studies suggest that antinuclear antibodies (ANAs) are frequently detected in patients with acute hepatitis A (AHA). However, the clinical...Full Text Available

2011-09-01

89

Cerebellar cell surface antigens of mouse brain.

Sera from multiple sclerosis patients with relapsing-remitting disease and normal subjects were tested for antibody to myelin basic protein by a sensitive radioimmunoassay. The results showed a marginally...Full Text Available

1978-12-01

90

Antibody protects against lethal infection with the neurally spreading reovirus type 3 (Dearing).

Reaggregated cells from 6- to 8-day-old mouse cerebella have been used to raise antibodies in rabbits. The interaction of these antibodies with cerebellar cell surface components was assessed by cytotoxicity...Full Text Available

1975-10-01

91

Antibodies against gonadotropin-releasing hormone (GnRH) and destruction of enteric neurons in 3 patients suffering from gastrointestinal dysfunction

The mammalian reoviruses have provided a valuable model for studying the pathogenesis of viral infections of the central nervous system (CNS). We have used this model to study the effect of antibody...Full Text Available

1988-12-01

92

Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating

BackgroundAntibodies against gonadotropin-releasing hormone (GnRH) and gastrointestinal dysmotility have been found after treatment with GnRH analogues. The aim of this study was...Full Text Available

93

A naturally occurring human antibody to loops of Henle

Botulism is caused by the botulinum neurotoxins (BoNTs), the most poisonous substance known. Because of the high potency of BoNT, development of diagnostic and therapeutic antibodies for botulism requires...Full Text Available

2010-04-01

94

A double antibody solid phase assay for DNA autoantibodies for clinical use.

A hitherto unreported immunofluorescent staining pattern obtained from human sera is described. Serum from six patients was found to possess specific antibody activity against lining cells of loops...Full Text Available

1973-08-01

95

Fine mapping of a foot-and-mouth disease virus epitope recognized by serotype-independent monoclonal antibody 4B2.

DNA antoantibodies in serum will bind to antigen-coated polystyrene tubes and can be detected by radiolabelled anti-immunoglobulin. The method is quantitative, gives information on the antibody class...Full Text Available

1976-08-01

96

Effects of chelate rigidity and density on the in vivo performance of [sup 203]Pb and [sup 88]Y labeled monoclonal antibodies

Full Text Available

2011-02-01

97

Sensitive enzyme immunoassay for detecting immunoglobulin M antibodies to Sindbis virus and further evidence that Pogosta disease is caused by a western equine encephalitis complex virus.

Short communication.

1993-01-01

98

Reduced "9"9"mTc labelled NCA-95/CEA-antibody uptake in liver due to gentle antibody reconstitution

An antibody capture enzyme immunoassay (EIA) was adapted for the detection of immunoglobulin M (IgM) antibody to Sindbis (SIN) virus. Sera from humans with a febrile illness characterized by rash and...Full Text Available

1985-10-01

99

Preparation and Properties of Antibodies against Indoleacetic Acid (IAA)-C5-BSA, a Novel Ring-Coupled IAA Antigen, as Compared to Two Other Types of IAA-Specific Antibodies ¹

The influence of reconstituting a murine monoclonal IgG_1 antibody kit with pertechnetate Tc99m on antibody distribution in the liver, spleen and sternal bone marrow of patients was examined. The "9"9"mTc-labelled antibody used is directed against non-specific cross-reacting antigen (NCA-95) and carcinoembryonic antigen (CEA) and has been successfully applied for imaging tissue inflammation and bone marrow scanning. Radioactivity uptake was determined in the liver, spleen, bone marrow and a precordial background region in a consecutive series of 25 patients, examined with an antibody preparation, routinely radiolabelled according to the manufacturer's recommendations and in 14 patients, in whom the antibody was reconstituted with special care, avoiding bubble formation and dropping of buffer into the antibody-containing vial. Gentle compared with routine ...

100

Near-Infrared Fluorescence Labeled Anti-TAG-72 Monoclonal Antibodies for Tumor Imaging in Colorectal Cancer Xenograft Mice

Two types of indoleacetic acid (IAA) antigens have been described in the literature which show marked differences with respect to antibody specificity. In this communication an alternative antigen design...Full Text Available

1989-04-01

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101

Monoclonal antibody imaging in malignant and benign gastrointestinal diseases

Anti-TAG-72 monoclonal antibodies target the tumor-associated glycoprotein (TAG)-72 in various solid tumors. This study evaluated the use of anti-TAG-72 monoclonal antibodies, both murine CC49...Full Text Available

2009-01-01

102

Comparison of indirect immunofluorescent-antibody assay, enzyme-linked immunosorbent assay, and Western immunoblot for the diagnosis of Lyme disease in dogs.

The "1"3"1I-labelled monoclonal antibodies 791T/36 and 79IT/36 Fab fragments have been evaluated in the imaging of patients with colorectal cancer, with benign colorectal tumours, and with malignant tumours of other parts of the gastrointestinal tract. The results of clinical imaging have been carefully correlated with clinical findings and direct measurement of the preferential uptake of antibody by the tumours. (UK).

103

Comparative Study of ¹²⁵I- and [³H]Acetate-Labeled Antibodies in Detecting Iridescent Viruses

Enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescent-antibody assay (IFA), and Western immunoblot were used to test serum samples from 128 dogs for the presence of antibody to Borrelia...Full Text Available

1990-01-01

104

Role of the Yersinia pestis Ail Protein in Preventing a Protective Polymorphonuclear Leukocyte Response During Bubonic Plague.

Radioimmunoassays for detecting cell-associated or released virus are described using either ¹²⁵I- or [³H]acetate-labeled antibodies. In the first assay system, antigen-antibody...Full Text Available

1974-07-01

105

Platelet thrombosis in cardiac-valve prostheses

The ability of Yersinia pestis to forestall the mammalian innate immune response is a fundamental aspect of plague pathogenesis. In this study, we examined the effect of Ail, a 17-kDa outer membrane protein that protects Y. pestis against complement-mediated lysis, on bubonic plague pathogenesis in mice and rats. The Y. pestis ail mutant was attenuated for virulence in both rodent models. The attenuation was greater in rats than in mice, which correlates with the ability of normal rat serum, but not mouse serum, to kill ail-negative Y. pestis in vitro. Intradermal infection with the ail mutant resulted in an atypical, subacute form of bubonic plague associated with extensive recruitment of polymorphonuclear leukocytes (PMN or neutrophils) to the site of infection in the draining lymph node and the formation of large purulent abscesses that contained the bacteria. Systemic spread and mortality were greatly attenuated, however, and a productive adaptive immune response was generated ...

2011-10-01

106

The RADionuclide transport, removal, and dose (RADTRAD) code

The contribution of platelets and clotting factors in thrombosis on cardiovascular prostheses had been quantified with several tracers. Thrombus formation in vivo could be measured semiquantitatively in animal models and patients with indium-111, Technetium-99m labeled platelets, iodine-123, iodine-131 labeled fibrinogen, and In-111 and Tc-99m labeled antibody to the fibrinogen-receptor on the platelet- membrane, or fibrin. The early studies demonstrated that certain platelet-inhibitors, e.g. sulfinpyrazone, aspirin or aspirin- persantine increased platelet survival time with mechanical valves implanted in the baboon model and patients. Thrombus localization by imaging is possible for large thrombus on thrombogenic surface of prosthesis in the acute phase. The majority of thrombus was found in the sewing ring (Dacron) in the acute phase in both the mechanical and tissue valves. The amount of retained thrombus in both mechanical and tissue ...

1989-06-08

107

The RADionuclide Transport, Removal, and Dose (RADTRAD) code

The RADionuclide Transport, Removal, And Dose (RAD-TRAD) code is designed for U.S. Nuclear Regulatory Commission (USNRC) use to calculate the radiological consequences to the off-site population and to control room operators following a design-basis accident at light water reactor (LWR) power plants. This code utilizes updated reactor accident source terms published in draft NUREG-1465. The code will track the transport of radionuclides as they are released from the reactor pressure vessel, travel through the primary containment and other buildings, and are released to the environment. As the radioactive material is transported through the primary containment and other buildings, credit for several removal mechanisms may be taken, including sprays, suppression pools, overlying pools, filters, and natural deposition. Simple models are available for these different removal mechanisms that use, as input, information about the conditions in the plant and predict either ...

1993-11-14

108

$\\gamma$-ray bursts from internal shocks in a relativistic wind temporal and spectral properties

The RADionuclide Transport, Removal, And Dose (RADTRAD) code is designed for US Nuclear Regulatory Commission (USNRC) use to calculate the radiological consequences to the offsite population and to control room operators following a design-basis accident at Light Water Reactor (LWR) power plants. This code utilizes updated reactor accident source terms published in draft NUREG-1465, ``Accident Source Terms for Light-Water Nuclear Power Plants.`` The code will track the transport of radionuclides as they are released from the reactor pressure vessel, travel through the primary containment and other buildings, and are released to the environment. As the radioactive material is transported through the primary containment and other buildings, credit for several removal mechanisms may be taken including sprays, suppression pools, overlying pools, filters, and natural deposition. Simple models are available for these different removal mechanisms that use, as input, ...

1993-07-01

109

Suppression of Magnetization Ringing After Domain Wall Collision Studied by Micromagnetic Simulation

We construct models for gamma-ray bursts where the emission comes from internal shocks in a relativistic wind with a highly non uniform distribution of the Lorentz factor. We follow the evolution of the wind using a very simplified approach where a large number of layers interact by direct collisions but where all pressure waves have been suppressed. We suppose that the magnetic field and the electron Lorentz factor reach large equipartition values in the shocks. Synchrotron photons emitted by the relativistic electrons have a typical energy in the gamma-ray range in the observer frame. Synthetic bursts are constructed as the sum of the contributions from all the internal elementary shocks and their temporal and spectral properties are compared to the observations. We reproduce the diversity of burst profiles, the ``FRED'' shape of individual pulses and the short time scale variability. Synthetic bursts also satisfy the ...

1998-01-01

110

Specific Btk inhibition suppresses B cell? and myeloid cell?mediated arthritis

Full Text Available

2008-12-01

111

Some Medicinal Plants Suppressed Reproduction of Meloidogyne hapla on Codonopsis lanceolata Trautv

Abstract not provided

2011-08-29

112

Screening of Medicinal Plants to Suppress Population of Meloidogyne hapla in Codonopsis lanceolata Trautv

Full Text Available

2006-12-01

113

Non-model-based semi-active vibration suppression of stay cables using magneto-rheological fluid dampers

Full Text Available

2006-12-01

114

Construction and Verification of Useful Vectors for Ectopic Expression and Suppression of Plant Genes.

Not Available

2007-08-01

115

ADULT ACQUIRED TOLERANCE TO HOMOGRAFTS.

Full Text Available

2009-06-01

116

Rocky Mountain spotted fever in areas of high and low prevalence: survey for canine antibodies to spotted fever rickettsiae.

... The effectiveness of immune suppression with both 6-mercaptopurine and methotrexate was insufficient to permit induction of a clinically useful ...

1967-07-31

117

A comprehensive comparison of mixing, mass transfer, Chinese hamster ovary cell growth, and antibody production using Rushton turbine and marine impellers

Antibodies to Rickettsia rickettsii were detected by indirect immunofluorescence in sera from 149 of 467 dogs (32%) examined from 4 military installations located in Kentucky, North Carolina, Pennsylvania, and Virginia. The prevalence at individual installations ranged from 4.3% at Fort Knox, Ky, to 63.4% at Fort Bragg, NC. Most of the seropositive dogs were in the working and sporting groups of dogs. The difference in antibody prevalence between sexes was not significant. Serologic responses were related to R rickettsii infection, although antibodies to R montana also were detected in a few of the sera. Comparison of serodiagnostic methods indicated that the indirect fluorescent antibody test was more sensitive than was the indirect hemagglutination test for obtaining survey data on the prevalence of Rocky Mountain spotted fever in the area. PMID:6808878

1982-08-01

118

British Library Electronic Table of Contents (United Kingdom)

Large scale production of monoclonal antibodies has been accomplished using bioreactors with different length to diameter ratios, and diverse impeller and sparger designs. The differences in these physical attributes often result in dissimilar mass transfer, mechanical stresses due to turbulence and mixing inside the bioreactor that may lead to disparities in cell growth and antibody production. A rational analysis of impeller design parameters on cell growth, protein expression levels and subsequent antibody production is needed to understand such differences. The purpose of this study was to examine the impact of Rushton turbine and marine impeller designs on Chinese hamster ovary (CHO) cell growth and metabolism, and antibody production and quality. Experiments to evaluate mass transfer...

2011-01-01

119

The growth factor from plerocercoid larvae of the tapeworm, Spirometra mansonoides, stimulates growth but is not diabetogenic.

Characterization of monocyte differentiation-inducing (MDI) factors derived from human fetal membrane chorion cells undergoing apoptosis after influenza virus infection.

A factor produced by plerocercoids of the tapeworm Spirometra mansonoides is similar to human growth hormone (hGH) in that it stimulates body growth, binds to hGH receptors, cross-reacts with anti-hGH antibodies, and has lactogenic and insulin-like activities. The purpose of this study was to determine whether plerocercoid growth factor (PGF) is similar to hGH in expressing diabetogenic activity in the genetically obese (ob/ob) mouse. To determine an effective dose for use in the obese mice, the ability of daily injections of PGF to stimulate growth of phenotypically normal mice of the same strain was assessed in a 10-day weight gain assay. Injections of PGF stimulated a dose-dependent weight gain (r = 0.83) and 25 ng eq/day of PGF stimulated a response not significantly different from that produced by 100 micrograms of bovine growth hormone/day. Diabetogenicity was assessed using fasting blood glucose and glucose tolerance ...

1989-06-01

120

Estimation tests for effecting factor on decontamination property in crystallization process

Influenza virus infection during pregnancy has been implicated as one of cause of premature delivery, abortion and stillbirth. We have reported that cultured human fetal membrane chorion cells undergoing apoptosis by influenza virus infection secrete unidentified heat-stable monocyte differentiation-inducing (MDI) factors. In this study, cellular, biological and immunochemical characteristics of MDI factors were investigated using human monocytic leukemia THP-1 cells by nitroblue tetrazolium reduction and cell adhesion assays. The treatment of THP-1 cells with culture supernatants from the influenza virus-infected chorion cells induced the nitroblue tetrazolium reduction ability, which was inhibited by the addition of superoxide dismutase and diphenyleneiodonium chloride, an inhibitor for reduced nicotinamide adenine dinucleotide phosphate oxidase. The phenomenon was also observed in human peripheral blood monocytes and histiocytic leukemia ...

2006-06-06

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121

Pre-therapy 18F-FDG PET quantitative parameters help in predicting the response to radioimmunotherapy in non-Hodgkin lymphoma

Crystallization procedure is considered to have adaptability to new reprocessing process based on the PUREX process because it has an advantage in recovering rather pure uranium from contaminated uranium solution without reagent. NEXT (New Extraction System for TRU Recovery) process has been developed by JNC, and applying the crystallization process unit to NEXT process has a capability to contribute to an improvement of economical efficiency and reduction of liquid waste in NEXT process. Thus following studies were carried out. In crystallization process unit, UNH (Uranyl Nitrate Hydrate)-crystals are washed by a nitric acid solution to get high decontamination factor, but the data on UNH-crystals dissolution by washing procedure is insufficient to evaluate the effectiveness of crystallization process unit. So, in this study, the effect of a nitric acid concentration to UNH-crystals dissolution and decontamination factor was tested. As ...

122

Human Mammary Luminal Epithelial Cells Contain Progenitors to Myoepithelial Cells

Radioimmunotherapy (RIT) is a new treatment option for patients with non-Hodgkin lymphoma (NHL). Response to RIT currently remains difficult to predict using conventional prognostic factors and could be refined using functional imaging. The goal of this work is to evaluate the value of 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) in predicting response to Yttrium 90-labeled monoclonal antibodies for patients with NHL. Thirty-five patients with NHL who had undergone 18F-FDG PET prior to RIT with either 90Y-ibritumomab tiuxetan (group A; n=17) or 90Y-epratuzumab tetraxetan (group B; n=18) were included in this retrospective study. Four functional criteria were determined for each tumour lesion in a given patient: maximum and mean standard uptake values (SUVmax and SUVmean), functional lesion volume (LVol) and total lesion glycolysis (TLG, product of the volume and the SUVmean). For each patient, we determined highest SUVmax and ...

2010-03-01

123

Monoclonal antibodies to antigens on human neutrophils, activated T lymphocytes, and acute leukemia blast cells

The origin of the epithelial and myoepithelial cells in the human breast has not been delineated. In this study we have addressed whether luminal epithelial cells and myoepithelial cells are vertically connected, i.e., whether one is the precursor for the other. We used a primary culture assay allowing preservation of basic phenotypic traits of luminal epithelial and myoepithelial cells in culture. The two cell types were then separated immunomagnetically using antibodies directed against lineage-specific cell surface antigens into at best 100% purity. The cellular identity was ascertained by cytochemistry, immunoblotting, and 2-D gel electrophoresis. Luminal epithelial cells were identified by strong expression of cytokeratins 18 and 19 while myoepithelial cells were recognized by expression of vimentin and {alpha}-smooth muscle actin. We used a previously devised culture medium (CDM4) that allows vigorous expansion of proliferative myoepithelial cells and also ...

1999-02-01

124

Simulations of a Gas-Filled Helical Muon Beam Cooling Channel

The authors describe the production of two mouse hybridomas secreting monoclonal antibodies to antigenic determinants of the surface membranes of human neutrophils, activated T lymphocytes, and acute leukemic blast cells. The degree of lymphocyte stimulation was estimated from incorporation of /sup 3/H-thymidine with parallel microculture. Monoclonal antibodies of supernatants of hybridoma cultures shown here reacted in both immunofluorescence test and cytotoxicity test with surface membrane antigens on the majority of neutrophils and PHA-activated peripheral blood lymphocytes from healthy subjects, but did not give positive reactions with unactivated lymphocytes, adherent monocytes, erythrocytes, and alloantigen-stimulated lymphocytes.

1987-11-01

125

On the glueball spectrum in O(a)-improved lattice QCD

A helical cooling channel (HCC) has been proposed to quickly reduce the six-dimensional phase space of muon beams for muon colliders, neutrino factories, and intense muon sources. The HCC is composed of a series of RF cavities filled with dense hydrogen gas that acts as the energy absorber for ionization cooling and suppresses RF breakdown in the cavities. Magnetic solenoidal, helical dipole, and helical quadrupole coils outside of the RF cavities provide the focusing and dispersion needed for the emittance exchange for the beam as it follows a helical equilibrium orbit down the HCC. In the work presented here, two Monte Carlo programs have been developed to simulate a HCC to compare with the analytic predictions and to begin the process of optimizing practical designs that could be built in the near future. We discuss the programs, the comparisons with the analytical theory, and the prospects for a HCC design with the capability to reduce the six-dimensional phase ...

2005-05-16

126

Novel high-throughput screening system for identifying STAT3-SH2 antagonists

We calculate the light `glueball' mass spectrum in N_f=2 lattice QCD using a fermion action that is non--perturbatively O(a) improved. We work at lattice spacings a~0.1 fm and with quark masses that range down to about half the strange quark mass. We find the statistical errors to be moderate and under control on relatively small ensembles. We compare our mass spectrum to that of quenched QCD at the same value of a. Whilst the tensor mass is the same (within errors), the scalar mass is significantly lighter in the dynamical lattice theory, by a factor of ~0.84 +/- 0.03. We discuss what the observed m_q dependence of this suppression tells us about the dynamics of glueballs in QCD. We also calculate the masses of flux tubes that wind around the spatial torus, and extract the string tension from these. As we decrease the quark mass we see a small but growing vacuum expectation value for the corresponding flux tube operators. This provides clear ...

2002-01-01

127

Modeling of boiling- and tow-phase flow in offset-strip-fin heat-exchanger geometries

Constitutive activation of the oncogenic transcription factor STAT3 frequently occurs in various human malignancies. STAT3 activation involves dimerization via intermolecular pTyr-SH2 interaction. Thus, antagonizing this interaction is a feasible approach to inhibit STAT3 activation for cancer therapy. In order to identify selective STAT3 inhibitors, we developed a biochemical HTS system based on AlphaScreen technology, which measures the abilities of test compounds to antagonize pTyr-SH2 interactions. We screened our chemical libraries using this system and identified 5,15-diphenylporphyrin (5,15-DPP) as a selective STAT3-SH2 antagonist. Selective inhibition of STAT3 nuclear translocation and DNA biding activity was observed in cells treated with 5,15-DPP. IL-6-dependent dimerization of STAT3, c-myc promoter binding and c-myc protein expression were all suppressed by 5,15-DPP, whereas no decrement in either expression or phosphorylation level ...

2009-03-13

128

High-efficiency RF suppression circuit for fluorescent lamp inverters using charge pump and partial smoothinf capacitors; Chagi ponpu shiki korikiritsu bubun heikatsu hoshiki wo mochiita keikoto inbatayo kochoha teigen kairo

The two-phase heat-transfer and pressure-drop characteristics of offset-strip-fin heat-exchanger geometries are examined using both experimental and analytical modeling techniques. Newly-obtained boiling-flow heat-transfer data are presented for two large-scale offset fin geometries at low-to-moderate wall-superheat conditions. Data for which nucleate boiling appeared to be completely suppressed were analyzed together with similar results for other offset fin surfaces to study the effects of channel dimensions on the convective evaporation component of the local heat transfer coefficient. Although these data were found to be well correlated in terms of modified forms of the F and Martinelli parameters used in previous studies of round-tube flows, a single F-factor correlation valid for all combinations of fin and channel dimensions did not appear to exist. The two-phase pressure-drop characteristics of offset-fin surfaces were found to be much ...

1988-01-01

129

Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy

A highly efficiency partial smoothing method is proposed to be used for inverters for 40W class household fluorescent inverters. In this system, the input current from the commercial AC power source is accumulated in a capacitor with comparatively small capacity as the electric charge, and then the lamp circuit is driven by the inverter utilizing the charging voltage of the capacitor and the charging voltage of the separately prepared partial smoothing capacitor. By this method, the RF harmonic components in the supply current can be decreased to improve the power factor with no complicated feedback controlling device. In addition, comparatively small capacity coils and capacitors can be used. The structure of the circuit and the operation of this proposed method are described, and the operational characteristic of the prototype circuit and the result of analysis are shown. Furthermore, problems and improving method of the actual circuit are investigated by an ...

1997-06-20

130

Dynamic behaviors of sulfur evolved in the gas phase from pyrolysis of six Chinese coals

The Drosophila hairy gene encodes a basic helix- loop-helix protein that functions in at least two steps during Drosophila development: (1) during embryogenesis, when it partakes in the establishment of segments, and (2) during the larval stage, when it functions negatively in determining the pattern of sensory bristles on the adult fly. In the rat, a structurally homologous gene (RHL) behaves as an immediate-early gene in its response to growth factors and can, like that in Drosophila, suppress neuronal differentiation events. Here, the authors report the genomic cloning of the human hairy gene homolog (HRY). The coding region of the gene is contained within four exons. The predicted amino acid sequence reveals only four amino acid differences between the human and rat genes. Analysis of the DNA sequence 5[prime] to the coding region reveals a putatitve untranslated exon. To increase the value of the HRY gene as a genetic marker and to assess ...

1994-03-01

131

Dielectric properties of fluxed barium titanate ceramics with zirconia additions

Dynamic behaviors of gaseous sulfur-containing compounds evolved from pyrolysis of six Chinese coals were studied in the temperature range of up to 800{sup o}C under N{sub 2} and H{sub 2}. The released amount of total sulfur-containing gases was traced by an online flame photometric detector (FPD). Simultaneously, the changes of different sulfur forms, including H{sub 2}S, SO{sub 2}, COS and CS{sub 2}, also were investigated using an online mass spectrometer (MS). FPD results show that the effect of H{sub 2} on gaseous sulfur evolved is complex, which promoted certain peak and suppresses other peaks. Based on the data from MS, it is suggested that a series of competitive reactions between active sulfur and other active matters during pyrolysis may impact on the sulfur form in gas phase. Interactions between active sulfur-containing intermediates and the coal matrix are attributed to be the main factor determining the dynamic behavior.

2006-07-01

132

Dielectric properties of fluxed barium titanate ceramics with zirconia additions

BaTiO{sub 3} compacts, when fluxed with {lt} 2 vol% of a complex borate glass phase, were sintered to near theoretical density at temperatures {lt} 1175{degrees}C in 2 h. Microstructural analysis showed a uniform grain size {lt} 1.0 {mu}m with 0.75 wt% ZrO{sub 2} added to the flux phase as a grain growth inhibitor. TEM analysis revealed a microcrystalline grain-boundary phase with the ZrO{sub 2} resident along the grain boundaries. These samples displayed an essentially flat dielectric profile, low dissipation factors ({lt} 2%) over the range 25{degrees} to 125{degrees}C, a near linear dependence ({approx} {+-} 15%) between 25{degrees} and {minus} 55{degrees}C, and significantly increased voltage stability. X-ray diffraction analysis of these small-grained materials indicates a suppression of the tetragonal structure toward a more cubic modification.

1990-03-01

133

Data Compression on Zero Suppressed High Energy Physics Data

BaTiO_3 compacts, when fluxed with < 2 vol% of a complex borate glass phase, were sintered to near theoretical density at temperatures < 1175 degrees C in 2 h. Microstructural analysis showed a uniform grain size < 1.0 #mu#m with 0.75 wt% ZrO_2 added to the flux phase as a grain growth inhibitor. TEM analysis revealed a microcrystalline grain-boundary phase with the ZrO_2 resident along the grain boundaries. These samples displayed an essentially flat dielectric profile, low dissipation factors (< 2%) over the range 25 degrees to 125 degrees C, a near linear dependence (#approx# #+-# 15%) between 25 degrees and - 55 degrees C, and significantly increased voltage stability. X-ray diffraction analysis of these small-grained materials indicates a suppression of the tetragonal structure toward a more cubic modification.

134

Common colorectal cancer risk variants in SMAD7 are associated with survival among prediagnostic nonsteroidal anti-inflammatory drug users: A population-based study of postmenopausal women

Future High Energy Physics experiments will produce unprecedented data volumes (up to 1 GB/s [1]). In most cases it will be impossible to analyse these data in real time and they will have to be stored on durable mostly magnetic linear media (e.g. tapes) for later analysis. This threatens to become a major cost factor for the running of these experiments. Here we present some ideas developed together with the Institute of Computer Graphics, Department for Algorithms and Programming on how this volume and the related cost can be reduced significantly. The algorithms presented are not general ones but aimed in particular to physics experiments data. Taking advantage of the knowledge of the data they are highly superior to general ones (Huffman, LZW, arithmetic coding) both in compression rate but more importantly in speed as to keep up with the output rate to modern tape drives. Above standard algorithms are, however, used after the data have been transferred in a ...

1996-01-01

135

British Library Electronic Table of Contents (United Kingdom)

Abstract Common single nucleotide polymorphisms (SNPs) in SMAD7 (18q21) have been linked to colorectal cancer (CRC) risk in genome-wide association studies, but little is known about their effects on survival. SMAD7 regulates gastrointestinal inflammation by inhibiting transforming growth factor- (TGFB), which can act as both a tumor suppressor and a promoter of metastasis. Regular use of cyclooxygenase-2 (COX2) inhibitors, such as nonsteroidal anti-inflammatory drugs (NSAIDs), reduces the risk of developing CRC. Because COX2 overexpression reduces the growth suppressing effects of TGFB, we hypothesized that survival may depend on both SMAD7 genotype and prediagnostic NSAID use. Postmenopausal women, ages 50-74, diagnosed with incident invasive CRC from 1997 to 2002 were identified using t...

2011-01-01

136

A DC-81-indole conjugate agent suppresses melanoma A375 cell migration partially via interrupting VEGF production and stromal cell-derived factor-1a-mediated signaling

British Library Electronic Table of Contents (United Kingdom)

Pyrrolo[2,1-c][1,4]benzodiazepine (PBD) chemicals are antitumor antibiotics inhibiting nucleic acid synthesis. An indole carboxylate-PBD hybrid with six-carbon spacer structure (IN6CPBD) has been previously demonstrated to induce melanoma cell apoptosis and reduce metastasis in mouse lungs. This study aimed at investigating the efficacy of the other hybrid compound with four-carbon spacer (IN4CPBD) and elucidating its anti-metastatic mechanism. Human melanoma A375 cells with IN4CPBD treatment underwent cytotoxicity and apoptosis-associated assays. Transwell migration assay, Western blotting, and ELISA were used for mechanistic study. IN4CPBD exhibited potent melanoma cytotoxicity through interrupting G1/S cell cycle progression, increasing DNA fragmentation and hypodipoidic DNA contents, a...

2011-01-01

137

Visualization of Tumor Angiogenesis Using MR Imaging Contrast Agent Gd-DTPA-anti-VEGF Receptor 2 Antibody Conjugate in a Mouse Tumor Model.

The inhibition of the primary interaction between "1"2"5I-labeled human hemoglobin and rabbit anti-human hemoglobin sera: a sensitive radioimmunoassay technique

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2010-07-01

138

... ammonium compounds antigen-antibody reactions hemoglobin inhibition

139

Reversing B cell aging

Postpartum spontaneous colonic perforation due to antiphospholipid syndrome

Age-related alterations in the cellular composition of the B lineage are a major cause of the poor antibody response to vaccination and to infectious agents among the elderly population. The mechanisms...Full Text Available

140

Neurobrucellosis in Stranded Dolphins, Costa Rica

The antiphospholipid syndrome (APS) is a multi-systemic disease being characterized by the presence of antiphospholipid antibodies that involves both arterial and venous systems resulting in arterial...Full Text Available

2009-01-28

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141

Isolation of antibodies specific to sickle hemoglobin by affinity chromatography using a synthetic peptide

Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. Brucella...Full Text Available

2008-09-01

142

Incidence and specificity of antibodies to types I, II, III, IV, and V collagen in rheumatoid arthritis and other rheumatic diseases as measured by 125I-radioimmunoassay

Antibodies to hemoglobin have been studied with a radioimmunoassay which employs ["1"4C]carbamylated (= carbamoylated) hemoglobin S. An antiserum raised against hemoglobin S, which initially discriminated poorly between hemoglobins S and A, was fractionated by absorption to a column of Sepharose to which a synthetic peptide corresponding to the first 13 amino-acid residues of the #beta# chain of sickle hemoglobin had been covalently bound. A subpopulation of the antiserum was eluted from this column with 4 M guanidine . HCl. These antibodies showed binding to hemoglobin S but not to hemoglobin A and this interaction could be inhibited by the synthetic peptide. These antibodies, of demonstrated fine structural specificity, may be useful in the detection of sickle hemoglobin and in the study of its structure in solution.

143

Human heterophilic antibodies against equine immunoglobulins: assessment of their role in the early adverse reactions to antivenom administration

Antibodies to human native and denatured types I, II, III, IV, and V collagens were measured using 125I-radioimmunoassay. Mean levels of binding by sera from 30 rheumatoid arthritis patients were significantly higher than those from 20 normal subjects against all of the collagens tested. The relative antibody concentration was higher in synovial fluid than in simultaneously obtained serum. Many patients with gout or various other rheumatic diseases also had detectable anticollagen antibodies. With a few notable exceptions, the majority of the reactivity detected in all patient groups was directed against covalent structural determinants present on all of the denatured collagens, suggesting a secondary reaction to tissue injury.

144

British Library Electronic Table of Contents (United Kingdom)

Summary The presence of human heterophilic antibodies against horse immunoglobulins (HHA-HI) was determined by ELISA in sera from healthy volunteers and from patients who received equine antivenom for therapy of snake bite envenoming. These patients were selected from two independent clinical studies: one in Colombia in which patients received antivenom constituted by whole IgG (n=25); and the other in Brazil where an antivenom constituted by F(abprime)2 fragments was administered (n=31). Results show that healthy volunteers have antibodies, mainly of the IgG class, able to react with whole equine IgG. Additionally, patients have IgG antibodies that react both with whole equine IgG and F(abprime)2 fragments. In both clinical studies, no significant differences were observed in the HHA-HI t...

2008-01-01

145

First report on antibody response of Seriola dumerilii (Risso 1810) challenged with Listonella anguillarum

British Library Electronic Table of Contents (United Kingdom)

The greater amberjack, Seriola dumerilii (Risso 1810) is a semi-pelagic fish and a worldwide species; it is considered a promising candidate for the diversification of Mediterranean aquaculture. In this paper an experimental injection with Listonella (Vibrio) anguillarum was performed to study the immune response of S. dumerilii. Antibody titres to L. anguillarum O1 were determined with indirect-ELISA at different times over a period of 42 days. Results showed that the antibody levels against L. anguillarum were significantly higher in the challenged fish compared to the control. They started developing since the 5th day reaching the highest peak on day 20 after injection, indicating a fast response of the immune system. The observed antibody titre was very high versus L. anguillarum if co...

2008-01-01

146

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

British Library Electronic Table of Contents (United Kingdom)

Abstract The glycosylation profile of therapeutic antibodies is routinely analyzed throughout development to monitor the impact of process parameters and to ensure consistency, efficacy, and safety for clinical and commercial batches of therapeutic products. In this study, unusually high levels of the mannose-5 (Man5) glycoform were observed during the early development of a therapeutic antibody produced from a Chinese hamster ovary (CHO) cell line, model cell line A. Follow up studies indicated that the antibody Man5 level was increased throughout the course of cell culture production as a result of increasing cell culture medium osmolality levels and extending culture duration. With model cell line A, Man5 glycosylation increased more than twofold from 12% to 28% in the fed-batch process...

2011-01-01

147

Apparatus for use in radioimmunoassays

Antibodies from malaria-exposed pregnant women recognize trypsin resistant epitopes on the surface of Plasmodium falciparum-infected erythrocytes selected for adhesion to chondroitin sulphate A

Apparatus for solid-phase antibody separation techniques used in radioimmunoassays is described in this invention. It consists of a rectangular prism tray with multiple wells protruding into its interior from one side. Near the base of the tray is an orifice used for creating evacuated condition within the structure. At the base of each well there is an orifice of such size and shape as to retain an aqueous liquid under given pressure conditions but permit the evacuation of this liquid at reduced pressure. The outlet of these orifices is in the shape of an inverted conical frustrum. Each of the wells contains an antibody coated disc of porous cellulose paper surrounded by a plastic support. The porous nature of the cellulose paper ensures contact between the antibody coating and the antigen. The use of antibody coated porous cellulose paper in combination with the vacuum operated apparatus simplifies ...

1991-08-26

148

A search for the epidemic typhus agent in Ethiopian ticks*

Udgivelsesdato: 2004-Sep-6

2004-01-01

149

A COMPARISON OF FOUR FLUORESCENT ANTIBODY-BASED METHODS FOR PURIFYING, DETECTING, AND CONFIRMING CRYPTOSPORIDIUM PARVUM ...

The presence of antibodies to Rickettsia prowazeki in domestic animals from several parts of Africa, and the isolation of this rickettsia from the blood of goats and sheep and from...Full Text Available

1973-01-01

150

... r2 = 0.9) in the data was a power model where the number of filters was equal to ... ...

151

Oligomycin-induced Bioenergetic Adaptation in Cancer Cells with Heterogeneous Bioenergetic Organization

Enteropathogenic E. coli were sought routinely by the fluorescent antibody technique, using monovalent and polyvalent conjugates (rhodamine sulfonyl· chloride, fluorescein and rhodamine isothiocyanate)....Full Text Available

1962-11-03

152

Mechanism of Host Defense Suppression Induced by Viral Infection: Mode of Action of Inosiplex as an Antiviral Agent

Cancer cells constantly adapt to oxidative phosphorylation (OXPHOS) suppression resulting from hypoxia or mitochondria defects. Under the OXPHOS suppression, AMP-activated protein kinase (AMPK) regulates...Full Text Available

2010-04-23

153

Long-term impact of acyclovir suppressive therapy on genital and plasma HIV RNA in Tanzanian women: a randomised controlled trial

The mechanism of influenza virus (INFV)-induced immunosuppression and the mode of inosiplex action against INFV infection were studied. INFV suppressed both anti-lipopolysaccharide and anti-sheep erythrocyte...Full Text Available

1982-10-01

154

Increased Sensitivity to Light-Induced Melatonin Suppression in Premenstrual Dysphoric Disorder

BackgroundHerpes simplex virus (HSV) suppressive therapy reduces genital and plasma HIV-1 RNA over periods up to three months but the longer-term effect is unknown.Full Text Available

2010-05-01

155

Triptolide-induced suppression of phospholipase D expression inhibits proliferation of MDA-MB-231 breast cancer cells.

Increased sensitivity to light-induced melatonin suppression characterizes some, but not all, patients with bipolar illness or seasonal affective disorder. The aim of this study was to test...Full Text Available

2010-08-01

156

Sidelobe Suppression in an Acousto-Optic Filer with a Raised ...

Full Text Available

2009-09-01

157

Suppression of afterglow in CsI:Tl by co doping with Eu"2"+-II: Theoretical model

... Abstract : The acousto-optic filter (AOF) is ... for large switch networks. ... FOURIER TRANSFORMATION, OPTICAL SWITCHING, SURFACE ACOUSTIC ...

1992-04-01

158

Radiochemistry of lead-203 for radiolabelling antibody conjugates

The mechanism for afterglow suppression in codoped CsI:Tl,Eu reported in the preceding paper was investigated by combined radioluminescence and thermoluminescence experiments. Model rate equations informed by these experiments were employed to simulate afterglow. It was found that codoping with europium introduces deep electron traps, with room-temperature glow peaks, that effectively scavenge the electrons from shallow traps associated with thallium, thus suppressing afterglow in the time domain of tens of milliseconds.

2006-03-15

159

Production and characterization of monoclonal antibodies recognizing the alpha-chain subunits of human ia alloantigens.

Lead-203 [52.1 h, 279 (80.1%) KeV] has been recognized as a potentially useful tracer for tumor specific radiopharmaceuticals due to its favorable nuclear and chemical properties. This paper reports the cyclotron production of lead-203 and the labelling of monoclonal antibody B72.3, conjugated with 2-(p-isothiocyanatobenzyl)DOTA, with lead-203 in 30% yield. In vivo biodistribution and stability studies in mice are being conducted.

1990-06-24

160

No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls

Two monoclonal antibodies, TAL-1B5 and TAL-3C3, specific for human Ia alpha-chain subunits have been produced by fusing P3/NSI/1-Ag4-1 mouse myeloma cells with spleen cells from a BALB/c mouse immunized...Full Text Available

1983-12-01

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161

British Library Electronic Table of Contents (United Kingdom)

New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env or the XMRV-gag antigen.

2011-01-01

162

Monoclonal antibodies to ovine SBU-T8 and SBU-T6 bind analogous molecules on bovine lymphocytes.

Double-antibody solid-phase radioimmunoassay: a simplified phase-separation procedure applied to various ligands

Monoclonal antibodies (mAb) to ovine T-lymphocyte molecules SBU-T8 (Maddox, Mackay & Brandon, 1985), the cytotoxic T lymphocyte, human CD8 equivalent, and SBU-T6 (Mackay et al., 1985), the immature...Full Text Available

1989-06-01

163

Double-antibody solid-phase radioimmunoassay: a simplified phase-separation procedure applied to various ligands

The purpose was to develop a simplified and reliable method of separating free from antibody-bound ligand using a precipitating antibody linked to a cellulose derivative. Dose-response curves and control sera were set up in parallel for various pituitary and placental polypeptides, steroid hormones, insulin, glucagon, triiodothyronine, thyroxine, angiotensin I, calcitonin, gastrin, cyclic AMP, and digoxin. After first-antibody reactions had reached equilibrium, free and bound ligand were separated using a double-antibody solid-phase system in parallel with conventional methods, including dextran-coated charcoal, double-antibody precipitation, single-antibody solid phase, organic solvents, salt precipitation, and anion-exchange resins. The effect of variations in temperature, incubation time, protein content, pH, and amount of separating material added were ...

1980-06-01

164

Characterization of DTPA complexes and conjugated antibodies of astatine

The purpose was to develop a simplified and reliable method of separating free from antibody-bound ligand using a precipitating antibody linked to a cellulose derivative. Dose-response curves and control sera were set up in parallel for various pituitary and placental polypeptides, steroid hormones, insulin, glucagon, triiodothyronine, thyroxine, angiotensin I, calcitonin, gastrin, cyclic AMP, and digoxin. After first-antibody reactions had reached equilibrium, free and bound ligand were separated using a double-antibody solid-phase system in parallel with conventional methods, including dextran-coated charcoal, double-antibody precipitation, single-antibody solid phase, organic solvents, salt precipitation, and anion-exchange resins. The effect of variations in temperature, incubation time, protein content, pH, and amount of separating material added were ...

165

Antibodies against a novel nucleolar and cytoplasmic antigen (p105-p42) present in the sera of patients with a subset of rheumatoid arthritis (RA) with signs of scleroderma

The complex formation of astatine(I) cation with diethylene triamine pentaacetic acid (DTPA) and characterization of the complexes were investigated by electromigration in free electrolytes and by gel-chromatography on Sephadex G 25. The conjugation procedure for the production of At-DTPA conjugated polyclonal antibodies is described. (author) 15 refs.; 2 figs.

166

Ultrasound and airflow induced thermal instability suppression of DC corona discharge: an experimental study

We identified three patients (two of them relatives) with RA and signs of scleroderma whose sera contained a high titre of IgG class antibodies against the nucleoli and the nucleoplasm of cells of different...Full Text Available

1998-11-01

167

Plerocercoid growth factor (PGF), a human growth hormone (hGH) analogue produced by the tapeworm Spirometra mansonoides, has direct insulin-like action in adipose tissue of normal rats in vitro

The effect of ultrasound waves, airflow and combined ultrasound with the airflow on the thermal instability suppression of a hollow needle-to-plate electrical discharge was studied experimentally. To evaluate the thermal instability suppression we used the V-A characteristics of the discharge in stationary air, with ultrasound applied in stationary air, and finally when the airflow was supplied into the discharge through the needle without and with ultrasound application. To illustrate the effect of ultrasound, airflow and combined ultrasound with airflow on the discharge thermal instability suppression we also studied the discharge ozone production. We found that in stationary air the application of ultrasound only slightly suppresses thermal instability. A substantial increase of ozone generation was not detected. Application of the airflow through the needle suppressed ...

2006-02-01

168

Plerocercoid growth factor (PGF), a human growth hormone (hGH) analogue produced by the tapeworm Spirometra mansonoides, has direct insulin-like action in adipose tissue of normal rats in vitro

The metabolic actions of GH can be divided into acute (insulin-like) and chronic (lipolytic/anti-insulin). The insulin-like actions of GH are most readily elicited in GH-deficient animals as GH induces resistance to its own insulin-like action. Like GH, PGF stimulates growth and cross-reacts with anti-hGH antibodies. Independent experiments were conducted comparing the direct actions of PGF to insulin or hGH in vitro. Insulin-like effects were determined by the ability of PGF, insulin or hGH to stimulate (U-/sup 14/C)glucose metabolism in epidydimal fat pads from normal rats and by inhibition of epinephrine-stimulated lipolysis. Direct stimulation of lipolysis was used as anti-insulin activity. To determine if PGF competes for insulin or GH receptors, adipocytes (3 x 10/sup 5/ cells/ml) were incubated with either (/sup 125/I)insulin or (/sup 125/I)hGH +/- PGF, +/- insulin or +/- hGH. PGF stimulated glucose oxidation and /sup 14/C-incorporation into lipids. Insulin, ...

1986-03-01

169

Nod2 sensing of lysozyme-digested peptidoglycan promotes macrophage recruitment and clearance of S. pneumoniae colonization in mice.

The metabolic actions of GH can be divided into acute (insulin-like) and chronic (lipolytic/anti-insulin). The insulin-like actions of GH are most readily elicited in GH-deficient animals as GH induces resistance to its own insulin-like action. Like GH, PGF stimulates growth and cross-reacts with anti-hGH antibodies. Independent experiments were conducted comparing the direct actions of PGF to insulin or hGH in vitro. Insulin-like effects were determined by the ability of PGF, insulin or hGH to stimulate [U-"1"4C]glucose metabolism in epidydimal fat pads from normal rats and by inhibition of epinephrine-stimulated lipolysis. Direct stimulation of lipolysis was used as anti-insulin activity. To determine if PGF competes for insulin or GH receptors, adipocytes (3 x 10"5 cells/ml) were incubated with either ["1"2"5I]insulin or ["1"2"5I]hGH +/- PGF, +/- insulin or +/- hGH. PGF stimulated glucose oxidation and "1"4C-incorporation into lipids. Insulin, hGH and PGF ...

1986-04-13

170

Genome Sequence of a Lancefield Group C Streptococcus zooepidemicus Strain Causing Epidemic Nephritis: New Information about an Old Disease

Streptococcus pneumoniae colonizes the mucosal surface of the human upper respiratory tract. A colonization event is gradually cleared through phagocytosis by monocytes/macrophages that are recruited to the airway lumen. Here, we sought to define the bacterial and host factors that promote monocyte/macrophage influx and S. pneumoniae clearance using intranasal bacterial challenge in mice. We found that the recruitment of monocytes/macrophages required their expression of the chemokine receptor CCR2 and correlated with expression of the CCR2 ligand CCL2. Production of CCL2 and monocyte/macrophage recruitment were deficient in mice lacking digestion of peptidoglycan by lysozyme (LysM) and cytosolic sensing of the products of digestion by Nod2. Ex vivo macrophages produced CCL2 following bacterial uptake, digestion by LysM, and sensing of peptidoglycan by Nod2. Sensing of digested peptidoglycan by Nod2 also required the pore-forming toxin pneumolysin. The generation ...

2011-08-15

171

Cloning of Drosophila transcription factor Adf-1 reveals homology to Myb oncoproteins.

Outbreaks of disease attributable to human error or natural causes can provide unique opportunities to gain new information about host-pathogen interactions and new leads for pathogenesis research. Poststreptococcal glomerulonephritis (PSGN), a sequela of infection with pathogenic streptococci, is a common cause of preventable kidney disease worldwide. Although PSGN usually occurs after infection with group A streptococci, organisms of Lancefield group C and G also can be responsible. Despite decades of study, the molecular pathogenesis of PSGN is poorly understood. As a first step toward gaining new information about PSGN pathogenesis, we sequenced the genome of Streptococcus equi subsp. zooepidemicus strain MGCS10565, a group C organism that caused a very large and unusually severe epidemic of nephritis in Brazil. The genome is a circular chromosome of 2,024,171 bp. The genome shares extensive gene content, including many virulence factors, with genetically ...

2008-08-21

172

Ophidian envenomation strategies and the role of purines.

The Drosophila sequence-specific DNA binding protein, Adf-1, is capable of activating transcription of the alcohol dehydrogenase gene, Adh, and is implicated in the transcriptional control of other developmentally regulated genes. We have cloned the cDNA encoding Adf-1 by generating specific DNA probes deduced from partial amino acid sequence of the protein. Several cDNA clones encoding an extended open reading frame were isolated from a phage lambda library. The complete amino acid sequence of Adf-1 deduced from the longest cDNA reveals structural similarities to the putative helix-turn-helix DNA binding motif of Myb and Myb-related proteins. DNA sequence analysis of genomic clones and Northern blot analysis of mRNA suggest that Adf-1 is a single-copy gene encoding a 1.9-kb transcript. Purified recombinant Adf-1 expressed in Escherichia coli binds specifically to Adf-1 recognition sites and activates transcription of a synthetic Adh promoter in vitro in a manner indistinguishable from ...

1992-01-15

173

Interrelation between grain boundary characteristics and intergranular stress corrosion cracking of thermally treated alloy 690 and shot peened alloy 800 in high temperature acidic and alkaline solution

Snake envenomation employs three well integrated strategies: prey immobilization via hypotension, prey immobilization via paralysis, and prey digestion. Purines (adenosine, guanosine and inosine) evidently play a central role in the envenomation strategies of most advanced snakes. Purines constitute the perfect multifunctional toxins, participating simultaneously in all three envenomation strategies. Because they are endogenous regulatory compounds in all vertebrates, it is impossible for any prey organism to develop resistance to them. Purine generation from endogenous precursors in the prey explains the presence of many hitherto unexplained enzyme activities in snake venoms: 5'-nucleotidase, endonucleases (including ribonuclease), phosphodiesterase, ATPase, ADPase, phosphomonoesterase, and NADase. Phospholipases A(2), cytotoxins, myotoxins, and heparinase also participate in purine liberation, in addition to their better known functions. Adenosine contributes to prey immobilization ...

2002-04-01

174

Interrelation between grain boundary characteristics and intergranular stress corrosion cracking of thermally treated alloy 690 and shot peened alloy 800 in high temperature acidic and alkaline solution

To clarify the acidic and alkaline Intergranular Stress Corrosion Cracking (IGSCC) mechanism of thermally treated alloy 690 (alloy 690 TT) and shot peened alloy 800 (alloy 800 SP), C-ring tests were conducted in deaerated HCl solutions and in deaerated NaOH solutions at 350degC, compared with the acidic and the alkaline IGSCC susceptibilities of mill-annealed alloy 600 (alloy 600 MA), full-sensitized one (alloy 600 FS) and thermally treated one (alloy 600 TT). Grain boundary characteristics, such as chromium depleted zone and chromium carbide precipitation, were examined using modified Huey test and Transmission Electron Microscopy. Potential-pH diagram for Ni, Cr, Fe-H{sub 2}O system at 350degC was constructed and the solubilities of NiO, Cr{sub 2}O{sub 3} and Fe{sub 3}O{sub 4} were also calculated to evaluate the stability of oxide films which were formed on the surfaces of alloy 690, 800 and 600. Under the acidic condition, the IGSCC susceptibility of alloy 800 SP was high. The ...

2001-05-01

175

Neurite outgrowth effect of 4-O-methylhonokiol by induction of neurotrophic factors through ERK activation.

To clarify the acidic and alkaline Intergranular Stress Corrosion Cracking (IGSCC) mechanism of thermally treated alloy 690 (alloy 690 TT) and shot peened alloy 800 (alloy 800 SP), C-ring tests were conducted in deaerated HCl solutions and in deaerated NaOH solutions at 350degC, compared with the acidic and the alkaline IGSCC susceptibilities of mill-annealed alloy 600 (alloy 600 MA), full-sensitized one (alloy 600 FS) and thermally treated one (alloy 600 TT). Grain boundary characteristics, such as chromium depleted zone and chromium carbide precipitation, were examined using modified Huey test and Transmission Electron Microscopy. Potential-pH diagram for Ni, Cr, Fe-H_2O system at 350degC was constructed and the solubilities of NiO, Cr_2O_3 and Fe_3O_4 were also calculated to evaluate the stability of oxide films which were formed on the surfaces of alloy 690, 800 and 600. Under the acidic condition, the IGSCC susceptibility of alloy 800 SP was high. The selective dissolution of Fe ...

2001-05-01

176

Large orbital magnetic moment and its quenching in the itinerant uranium intermetallic compounds UTGa_5 (T=Ni, Pd, Pt)

Compounds isolated from Magnolia officinalis such as magnolol, honokiol and obovatol exhibit several pharmacological effects on CNS including depressant, anxiolytic and anticonvulsant effects, as well as neuroprotective effects against chemical and heat damages. Recently, honokiol was found to have a neurotrophic effect in fetal rat cortical neurons. In the present study, we show that 4-O-methylhonokiol, a novel compound from Magnolia officinalis, promotes neurite outgrowth in a concentration- dependent manner in rat embryonic neuronal cells. In parallel with the neurite outgrowth activity, the expression of neurite outgrowth marker proteins is also increased by treatment with 4-O-methylhonokiol. We also found that 4-O-methylhonokiol promotes the release of NGF and BDNF into cell culture medium. In addition, lower concentration of 4-O-methylhonokiol (1 and 2 lM) further enhanced neurite outgrowth and expression of neurite outgrowth marker proteins in the presence of NGF (50 ng/ml) or ...

2009-12-01

177

Insulin-like growth factor binding protein-7 (IGFBP7) functions as a potential tumor suppressor in hepatocellular carcinoma (HCC).

The crystal structure, lattice strain due to the antiferromagnetic ordering, and magnetic form factor in the itinerant 5f compounds UTGa_5 (T=Ni, Pd, Pt) have been studied by neutron scattering. High-resolution powder diffraction revealed that the tetragonality of the U-Ga layers increases down to the series of the transition metal element T. The integrated intensities of the antiferromagnetic reflections can be well explained with the Neel-type structure for UNiGa_5, whereas UPtGa_5 has the antiferromagnetic stacking of the ferromagnetically ordered uranium moments in the c plane. In both compounds the uranium moments orient along the c axis with moments of 0.75(5) and 0.32(5) #mu#_B for UNiGa_5 and UPtGa_5, respectively. No magnetic peak could be observed in the powder diffraction pattern of UPdGa_5 due to the small magnetic moment less than the experimental sensitivity. The orbital contributions in the magnetic form factor are reduced from ...

2003-12-01

178

Development of a new radiolabel (lead-203) and new chelating agents for labeling monoclonal anntibodies for imaging

PURPOSE: Hepatocellular carcinoma (HCC) is a highly virulent malignancy with no effective treatment thus requiring innovative and effective targeted therapies. The oncogene Astrocyte elevated gene-1 (AEG-1) plays a seminal role in hepatocarcinogenesis and profoundly downregulates insulin-like growth factor binding protein-7 (IGFBP7). The present study focuses on analyzing potential tumor suppressor functions of IGFBP7 in HCC and the relevance of IGFBP7 downregulation in mediating AEG-1 function.EXPERIMENTAL DESIGN: IGFBP7 expression was detected by immunohistochemistry in HCC tissue microarray and real-time PCR and ELISA in human HCC cell lines. Dual Fluorescence in situ hybridization was performed to detect loss of heterozygosity at IGFBP7 locus. Stable IGFBP7-overexpressing clones were established in the background of AEG-1-overexpressing human HCC cells and were analyzed for in vitro proliferation and senescence and in vivo tumorigenesis and ...

2011-09-16

179

Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells

High liver uptake and slow body clearance presently limit the usefulness of "1"1"1In labeled antibodies for tumor imaging. We have investigated "2"0"3Pb as an alternate and better antibody label. The DTPA and cyclohexyl EDTA (CDTA) conjugates of an anticolon carcinoma antibody, 17-1A were labeled (bicyclic anhydride method) with "2"0"3Pb and "1"1"1In with 60 and 90% labeling yields, respectively. The biodistribution of "2"0"3Pb-17-1A conjugates was compared with the corresponding "1"1"1In-labeled preparations and with "2"0"3Pb-DTPA, "2"0"3Pb-nitrate and nonrelevant antibody controls in normal and human tumor (SW948) xenografted nude mice at 24, and 96 hr. Lead-203-labeled CDTA and DTPA antibody conjugates gave similar in vivo distributions. Even though the lead bound to these chelate-antibody conjugates was more labile in serum and in vivo, compared to indium, ...

1988-05-22

180

British Library Electronic Table of Contents (United Kingdom)

In mammals, primordial germ cells (PGCs) are generated in the extra-embryonic epiblast, and thereafter migrate into the developing gonads. Following the development of the gonads to the testes or ovaries, germ cells mature into sperms or eggs. In the present study, we report production and characterization of monoclonal antibodies (MAb) that recognize PGCs. Extracts from E12.5 mouse embryonic gonads were immunized as an antigen, and hybridomas were generated using the rat medial iliac lymph node method. The hybridoma supernatants were screened by immunohistochemical analyses of E12.5 mouse embryonic sections. The antibody, referred to herein as MAb 5B5, provided strong signals on PGCs. Moreover, immunofluorescence analyses using a variety of the tissue sections of mouse embryos revealed th...

2010-01-01

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181

Limitation of immunohistochemical localization of estrogen receptor

Condition of research reactor spent nuclear fuel in wet storage

Although the radioreceptor method is widely used for estrogen receptor assay in human tissue, it has several limitations and a number of alternative methods are being explored. An immunohistochemical method of estrogen receptor assay using a specific antibody to estradiol has been proposed as a suitable alternative. The present study was designed to evaluate the validity of this method in detecting true estrogen receptors in human tumor tissue. Using radioiodinated antibody to estrogen, we have demonstrated that the estrogen antibody can detect the estrogen when it is bound to 4S type receptor but is unable to bind to estrogen when the hormone is bound to 8S type receptor. Our observations suggest that the immunohistochemical method of detection of intracellular cytosolic receptor for estrogen is not a suitable alternative to the currently used radioreceptor method.

1986-10-01

182

Effects of soil pH on rhizoctonia damping-off of sugar beet and disease suppression induced by soil amendment with crop residues

The condition of spent nuclear fuel (SNF) in wet storage at ten Soviet-designed research reactors has been assessed in the light of international experience in order to identify any associated safety issues. These reactors use Al-clad UO2-Al or U-Al alloy dispersion fuels of ?20% enrichment that were fabricated in Russia; the reactors have been in operation since 1955-70. Although originally sent for reprocessing, much of the SNF generated over the last 25-30 years has been stored in fuel storage pools (FSPs) of variable water quality. The external condition of wet-stored SNF assemblies from the reactors surveyed varied from significant failure due to galvanic corrosion that was driven by poor water quality, through gradual pitting caused by slightly impure water, to a stable condition of no observable change in the oxidized Al alloy surface of the irradiated fuel. SNF stability in wet storage seems to depend on three factors: Al being the sole metal in the FSP (to ...

2004-10-01

183

British Library Electronic Table of Contents (United Kingdom)

Effects of soil pH on damping-off of sugar beet by R. solani (AG2-2) and soil suppressiveness against the disease were studied by comparing disease incidences in pasteurized versus non-pasteurized, infested soils. Soil pH was correlated neither to disease incidence in five soils ranging from pH?4.5 to 7.2 nor to indigenous disease suppressiveness, the difference in disease incidences between non-treated soil and its pasteurized counterpart. When an alkaline soil was acidified with H2SO4, disease suppression markedly declined, increasing disease incidence in the non-pasteurized soil. Inversely, disease suppression was enhanced when an acidic soil was neutralized by adding Ca(OH)2. Soil amendment with dried peanut plant residue suppressed the disease in two pasteurized, near-neutral soils, l...

2011-01-01

184

{ital J}/{ital {psi}} Suppression in Pb-Pb Collisions: A Hint of Quark-Gluon Plasma Production?

The NA50 Collaboration has recently observed a strong suppression of {ital J}/{psi} production in Pb-Pb collisions at 158GeV/nucleon. We show that this recent observation finds a quantitative explanation in a model which relates the suppression mechanism to the local energy density, whose value is higher in Pb-Pb collisions than in any other system studied previously. The sensitivity of the phenomenon to small changes in the energy density could be suggestive of quark-gluon plasma formation. {copyright} {ital 1996 The American Physical Society.}

1996-08-01

185

Evidence for glueballs

Evaluation of efficacy of radiation-sterilized females for population suppression of Earias vittella (Fabricius)

This paper describes the observation and partial wave analysis of 1203 (22 GeV) #pi#"-p #-># phi phi n events. This is an OZI suppressed channel in which the OZI suppression is found to be absent. Assuming QCD and the OZI rule as Ansatzen, it is concluded that the breakdown of the OZI suppression is due to glueballs. The g/sub T/(2160) and the g/sub T/(2320) with I/sup G/J/sup PC/ = 0"+2"+"+ are two resonances determined from the partial wave analysis. It is concluded that one or two primary glueballs with the above quantum numbers are responsible for the observed two states. A brief discussion of other glueball candidates and some relevant phenomenology is also included.

1982-10-28

186

The vacuolar processing enzyme (VPE) mutation suppresses an HR-like cell death induced by the double knockout mutant of vacuolar Ca2+-ATPases in Arabidopsis

Feasibility of using sterile females for population suppression of Earias vittella (Fabricius) was evaluated by conducting studies on mating competitiveness of radiation sterilized females, both in individual pairs and in simulated large population conditions. Results showed that sterility induced by using a 10:1:1 ratio (sterile female: normal female: normal male) in single pair experiments and also in simulated large population conditions was more than 99.0 per cent. Release of radiation- sterilized females of E. vittella in natural populations has thus an excellent potential for population suppression. (author)

187

Structural alterations in the seminiferous tubules of rats treated with immunosuppressor tacrolimus

Full Text Available

2011-06-01

188

Jet Noise Modeling for Suppressed and Unsuppressed Aircraft in - NASA

BackgroundTacrolimus (FK-506) is an immunosuppressant that binds to a specific immunophilin, resulting in the suppression of the cellular immune response during transplant rejection....Full Text Available

189

Effect of minor alloying elements and their segregation on void suppression in modified 316 stainless steel

Mechanisms, AGARD Conference Proceedings No. 131, 1974, Paper No. 11. ... Olsen, W. and Karchmer, A., Lip Noise Generated by Flow Separation From Nozzle ...

190

Carnation Fusarium wilt suppression in four composts

The effects of modification with Ti and Nb, heat treatment and solute segregation on void suppression in 316 stainless steel were studied using HVEM in the temperature range of 623-873 K after solution treatment at 1323-1473 K. Post-irradiation compositional analysis was performed by an energy dispersive X-ray analyzer. In Ti-modified stainless steel, void swelling peaks appeared at 723 K and 823 K. On the other hand, when the stainless steels modified by both Ti and Nb were irradiated, the void swelling was effectively suppressed, particularly the growth rate of a void was remarkedly retarded. This void suppression effect was easily recognized with increasing Nb concentration. Compositions such as Mo, Cr, Ti and Ni changed during irradiation and Ni, Si and Ti enriched around voids, whereas Cr and Mo depleted. (orig.).

1983-09-01

191

British Library Electronic Table of Contents (United Kingdom)

Fusarium wilt is now a major disease of carnation crops worldwide. Methyl bromide, which is used to remedy it, is environmentally unsafe. An alternative approach integrated into biological control is to grow crops in suppressive media. Suppressiveness of seven plant growth media to Fusarium oxysporum f. sp. dianthi was evaluated in bioassays with carnation (Dianthus cariophyllus) cv. Medea. These media were: (1) grape marc compost, (2) cork compost, (3) olive oil husk + cotton gin trash composted and mixed with rice husk, (4) spent mushroom compost mixed with peat, (5) coir fibre, (6) light peat and (7) vermiculite. In order to look for carnation Fusarium wilt suppressiveness indicators, growth medium pH and ?-glucosidase activity were evaluated. Furthermore, F. oxysporum populations were ...

2009-01-01

192

A fuel treatment reduces fire severity and increases suppression efficiency in a mixed conifer forest

The first international standard for antibodies to double stranded DNA.

Fuel treatments are being implemented on public and private lands across the western United States. Although scientists and managers have an understanding ... ...

193

The effect of an ?-globulin preparation and of polyribonuclease complexes on humoral antibody formation

This paper announces the availability of the first international standard for anti-double-stranded DNA (anti-dsDNA). The material, coded Wo/80, was obtained after recalcification of plasma taken from...Full Text Available

1988-09-01

194

The Structure of the Dust Mite Allergen Der p 7 Reveals Similarities to Innate Immune Proteins

The effects of bovine α-globulin (BAG), bovine-ribonuclease serum albumin (BSA-RNase) and polyribonuclease (poly-RNase) complexes on the primary response of rodents to sheep erythrocytes have...Full Text Available

1968-06-01

195

The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination

BackgroundSensitization to house dust mite allergens is strongly correlated with asthma. Der p 7 elicits strong IgE antibody and T-cell responses in mite allergic...Full Text Available

2010-04-01

196

Tat-Neutralizing Antibodies in Vaccinated Macaques

Activation-induced deaminase (AID) initiates somatic hypermutation, gene conversion and class switch recombination by deaminating variable and switch region DNA cytidines to uridines. AID is predominantly...Full Text Available

197

Spontaneous coronary artery dissection in the postpartum period: association with antiphospholipid antibody

The human immunodeficiency virus Tat protein is essential for virus replication and is a candidate vaccine antigen. Macaques immunized with Tat or chemically modified Tat toxoid having the same clade...Full Text Available

2003-03-01

198

Serum Concentrations of Antibodies Against Vaccine Toxoids in Children Exposed Perinatally to Immunotoxicants

Spontaneous coronary artery dissection (SCAD) is an extremely uncommon cause of myocardial infarction, occurring predominantly in women during or after pregnancy. The exact aetiology...Full Text Available

2004-09-01

199

Scarce resources for nuclear... [Disaster Med Public Health Prep...

BackgroundPolychlorinated biphenyls (PCBs) may cause immunotoxic effects, but the detailed dose–response relationship and possible vulnerable time windows of exposure are...Full Text Available

2010-10-01

200

Safety and immunogenicity of a vaccine bait containing ERA strain of attenuated rabies virus.

and antibody responses of rhesus macaques exposed to the human gammaretrovirus XMRV. J Virol. 2011 May ;85(9):4547-57. Epub 2011 Feb 16 . PubMed Your browsing activity is...

2011-10-15

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201

Role of IgG and complement component C5 in the initial course of experimental cryptococcosis.

Ninety percent of foxes fed commercial ERA vaccine in a specially designed bait developed rabies serum neutralizing antibodies. The vaccine bait did not cause clinical signs of rabies when consumed...Full Text Available

1987-10-01

202

Passive immunity to feline leukemia: evaluation of immunity from dams naturally infected and experimentally vaccinated.

Although cellular immunity has a crucial role during cryptococcosis, several in vitro studies have pointed out the importance of IgG anti-Cryptococcus neoformans antibodies and complement components...Full Text Available

1989-12-01

203

Naturally occurring double-stranded RNA and immune responses. Effects on plaque-forming cells and antibody formation.

Antibodies against feline leukemia virus (FeLV) and the feline oncornavirus-associated cell membrane antigen (FOCMA) were transferred from pregnant cats to their suckling kittens. All of these kittens...Full Text Available

1977-04-01

204

Muscle-Specific Calpain is Localized in Regions Near Motor Endplates in Differentiating Lobster Claw Muscles

A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its...Full Text Available

1975-03-01

205

Lymphoma depletion during CD20 immunotherapy in mice is mediated by macrophage Fc?RI, Fc?RIII, and Fc?RIV

Calpains are Ca²⁺-dependent proteinases that mediate protein turnover in crustacean skeletal muscles. We used an antibody directed against lobster muscle-specific calpain (Ha-CalpM)...Full Text Available

2007-11-01

206

Low-frequency sound transmission through a g... [J Acoust Soc...

Despite the demonstrated clinical efficacy of CD20 monoclonal antibody (mAb) for lymphoma therapy, the in vivo mechanisms of tumor depletion remain controversial and variable. To identify the molecular...Full Text Available

2008-08-15

207

Location of an epitopic site on epiglycanin by molecular immunoelectron microscopy.

and antibody responses of rhesus macaques exposed to the human gammaretrovirus XMRV. J Virol. 2011 May ;85(9):4547-57. Epub 2011 Feb 16 . PubMed Surgical staging of early...

2011-10-15

208

Increased prevalence of antibodies to enteropathogenic Yersinia enterocolitica virulence proteins in relatives of patients with autoimmune thyroid disease

Antibodies of the IgM type present in rabbit anti-epiglycanin antiserum were purified by (NH4)2SO4 precipitation and by ion-exchange, affinity and gel-filtration chromatography. After papain treatment...Full Text Available

1985-04-01

209

Increased prevalence of Borrelia burgdorferi infections in Bernese Mountain Dogs: a possible breed predisposition

Infections have been implicated in the pathogenesis of a number of autoimmune diseases, and Yersinia enterocolitica (YE) might play a role in the development of autoimmune thyroid disease...Full Text Available

2003-05-01

210

In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.

BackgroundGlomerulonephritis in dogs has been associated with B. burgdorferi infections. In Bernese Mountain Dogs with glomerulonephritis antibodies against B....Full Text Available

211

Immunoreactive properties of anti-thyroglobulin autoantibodies isolated by affinity chromatography from human thyroiditis serum.

An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly...Full Text Available

1986-08-01

212

Immunochemical Analysis of the Temporal and Tissue-Specific Expression of an Avena sativa Plasma Membrane Determinant 1

A Sepharose-coupled 19S human thyroglobulin has been used as an immunoadsorbent to isolate anti-thyroglobulin autoantibodies and to evaluate the antigen-antibody interactions. With the system proposed...Full Text Available

1978-02-01

213

Immunization of foxes by the intestinal route using an inactivated rabies vaccine.

An immunoglobulin Mk monoclonal (F8IVE9) antibody raised against oat (Avena sativa cv Garry) root homogenate has been produced and characterized. The predominant target...Full Text Available

1992-01-01

214

IgE antibodies to D. pteronyssinus in atopic patients.

Approximately 30% of foxes given two doses of an inactivated rabies antigen delivered directly into the intestinal tract developed an immune response as measured by rabies serum neutralizing antibodies....Full Text Available

1989-01-01

215

Identification of the Neoplastically Transformed Cells in Marek's Disease Herpesvirus-Induced Lymphomas: Recognition by the Monoclonal Antibody AV37

An aqueous house dust mite extract was separated by SDS-PAGE and transferred to nitrocellulose membranes by Western Blotting. Two major allergens, Der pI and Der pII associated with the mite faeces...Full Text Available

1988-06-01

216

Hepatitis B antigen and antibody in the blood of prostitutes visiting an outpatient venereology department in Rotterdam.

Understanding the interactions between herpesviruses and their host cells and also the interactions between neoplastically transformed cells and the host immune system is fundamental to understanding...Full Text Available

2002-07-01

217

From antibody insult to fibrosis in neonatal lupus - the heart of the matter

We took blood samples from 128 prostitutes visiting the outpatient venereology department of the University Hospital, Rotterdam-Dijkzigt to test for the presence of hepatitis B surface antigen (HBsAg)...Full Text Available

1984-10-01

218

Followup study of possible HIV seropositivity among abusers of parenteral drugs in 1971-72.

Few diseases exemplify the integration of research from bench to bedside as well as neonatal lupus, often referred to as a model of passively acquired autoimmunity. In essence, this disease encompasses...Full Text Available

2003-01-01

219

Evidence of synthesis by Lumbricus terrestris of specific substances in response to an immunization with a synthetic hapten.

Serum specimens obtained from a nationwide sample of parenteral drug abusers (PDAs) during the period 1971-72 had previously been screened for human immunodeficiency virus (HIV) antibodies. Some specimens...Full Text Available

1991-07-01

220

Evaluation of solid phase system in radioimmunoassay of triiodothyronine (Preprint No. RA.24)

Lumbricus terrestris (Annelid, Oligocheta) is capable of cellular- and humoral-specific reactions against natural antigens. Is this earthworm able to elaborate a response of antibody type against a...Full Text Available

1985-12-01

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221

Establishment of an Immunoglobulin M Antibody-Forming Cell Response Model for Characterizing Immunotoxicity in Primary Human B Cells

This paper describes the evaluation of antibody coated polystyrene beads using "1"2"5I-labelled T_3 ("1"2"5I-T_3). (author).

222

Determination of antibody response to influenza virus surface glycoproteins by kinetic enzyme-linked immunosorbent assay.

Rodent models have been extensively utilized to identify putative human immunotoxicants; however, even when immunotoxicity is established, uncertainty remains whether the effects are predictive of human...Full Text Available

2009-12-01

223

Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay.

We modified an existing enzyme-linked immunosorbent assay (ELISA) to be able to use new spectrophotometers which can measure the rate of color development in microtiter wells. This new kinetic-based...Full Text Available

1988-10-01

224

Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies.

Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures...Full Text Available

1991-07-01

225

Comparative Medicine - National Center for Research Resources...

The authors have recently identified a new cytokeratin (CK) polypeptide, CK 20, whose expression is almost entirely confined to the gastric and intestinal epithelium, urothelium, and Merkel cells. Seven...Full Text Available

1992-02-01

226

Characterization of antibodies mediating protection and cure of Trypanosoma musculi infection in mice.

and Antibody Responses of Rhesus Macaques Exposed to the Human Gammaretrovirus XMRV external link, opens in new window J Virol. 2011 May;85(9):4547-57 Detection of CWD...

2011-10-15

227

Application of an indirect immunofluorescence test for detection of Mycoplasma pneumoniae in respiratory exudates.

Plasma samples were collected from mice infected with Trypanosoma musculi at different times postinfection and administered to naive recipient mice either before or during T. musculi infection. The...Full Text Available

1985-06-01

228

Antigenic analysis of the second extra-cellular loop of the human beta-adrenergic receptors.

We prepared polyclonal antibody specific to Mycoplasma pneumoniae and examined the conditions influencing the ability of an indirect immunofluorescence test to detect the specific antigen in respiratory...Full Text Available

1991-09-01

229

Antibodies to synthetic peptides from the tubulin regulatory domain interact with tubulin and microtubules.

Polyclonal antibodies were raised in rabbits by immunization with free peptides corresponding to positions 197-222 of the human beta 1-adrenergic receptor (beta 1 peptide) and the corresponding sequence...Full Text Available

1989-10-01

230

An Immunomagnetic Single-Platform Image Cytometer for Cell Enumeration Based on Antibody Specificity?

The carboxyl-terminal region of tubulin alpha and beta subunits plays a major role in regulating its assembly into microtubules and constitutes an essential domain for the selective interaction of microtubule-associated...Full Text Available

1988-09-01

231

A Serological Survey of Sera from Domestic Animals on Easter Island

Simplification of cell enumeration technologies is necessary, especially for resource-poor countries, where reliable and affordable enumeration systems are greatly needed. In this paper, an immunomagnetic...Full Text Available

2007-04-01

232

Status of bismuth and lead for radioimmunoimaging and therapy

Animals' sera collected on Easter Island from December 1964 to February 1965 were tested by appropriate methods for the presence of antibodies to various infections. These included, ornithosis, Q-fever,...Full Text Available

1968-04-01

233

Immunological detection and quantification of DNA components structurally modified by alkylating carcinogens, mutagens and chemotherapeutic agents

Of the radionuclides with physical properties most suited for use with monoclonal antibody for tumor imaging or therapy, few are more available or desirable than Pb-203, 212 and Bi-212. Yet no clinical studies have as yet been possible solely due to the lability in vivo of known complexes. We now report that bismuth complexes of the ligands p-SCN-Bz-DOTA and p-SCN-Bz-trans-cyclohexylDTPA are effective at protecting Bi-206 from release when linked to immunoproteins. Practicable, efficient ligand syntheses will be reported. Initial studies reveal that antibody is labeled efficiently with radiolead DOTA complexes. Preliminary studies indicate stability in vivo.

1990-08-26

234

Immunological correlates for protection against intranasal challenge of Bacillus anthracis spores conferred by a protective antigen-based vaccine in rabbits.

The detection and quantification of defined reaction products of chemical mutagens and carcinogens (and of many cancer chemotherapeutic agents) with DNA require highly sensitive analytical techniques. The exceptional capability of immunoglobulins to recognize subtle alterations of molecular structure (especially when monoclonal antibodies are used to maximize specificity), outstanding sensitivity of immunoanalysis by high-affinity antibodies, and the fact that radioactively-labelled agents are not required suggest the utility of a radioimmunoassay to recognize and quantitate alkylated DNA products. We have recently developed a set of high-affinity monoclonal antibodies (secreted by mouse x mouse as well as by rat x rat hybridomas; antibody affinity constants, 10"9 to > 10"1"0 lmol) specifically directed against several DNA alkylation products with possible relevance in relation to both mutagenesis ...

1983-09-01

235

Squamous cell carcinoma of the oropharynx: Ki-67 and p53 can identify patients at high risk for local recurrence after surgery and postoperative radiotherapy

Correlates between immunological parameters and protection against Bacillus anthracis infection in animals vaccinated with protective antigen (PA)-based vaccines could provide surrogate markers to evaluate the putative protective efficiency of immunization in humans. In previous studies we demonstrated that neutralizing antibody levels serve as correlates for protection in guinea pigs (S. Reuveny et al., Infect. Immun. 69:2888-2893, 2001; H. Marcus et al., Infect. Immun. 72:3471-3477, 2004). In this study we evaluated similar correlates for protection by active and passive immunization of New Zealand White rabbits. Full immunization and partial immunization were achieved by single and multiple injections of standard and diluted doses of a PA-based vaccine. Passive immunization was carried out by injection of immune sera from rabbits vaccinated with PA-based vaccine prior to challenge with B. anthracis spores. Immunized rabbits were challenged by intranasal spore ...

2006-01-01

236

Purpose: To assess the prognostic value of biologic (p53, Ki-67) and clinical factors in squamous cell carcinoma of the oropharynx after radical surgery and postoperative radiotherapy (RT). Methods and Materials: Between 1985 and 1995, a total of 102 patients with 104 tumor sites were entered onto the study. Fifty-five primary tumors (53%) involved the tonsils, 26 (25%) the soft palate, and 23 (22%) the base of the tongue. Median age was 53 years (range 36-80 years). The clinical T- and N-categories (UICC 1997) were: T1 (30), T2 (47), T3 (22), T4 (5), N0 (33), N1 (28), N2 (42), and N3 (1). Histologically-clear margins were achieved in all patients by initial surgery. Postoperative RT to the primary and regional lymphatics was given, to a total of 60 Gy in 6 weeks, and single daily fractions of 2 Gy. The expression of the nuclear p53- and Ki-67-labeling index (LI) was investigated by immunostaining using the monoclonal antibodies DO-7 and MIB 1. ...

2000-11-01

237

Social environment and steroid hormones affect species and sex differences in immune function among voles.

The IRG protein Irgm3 preserves cell survival during coxsackievirus B3 (CVB3) infection. However, the molecular mechanisms are not clear. Here, we examined the effect of Irgm3 expression on ER stress triggered by pharmacological agents or CVB3 infection. In Tet-On/Irgm3 HeLa cells, Irgm3 expression suppressed either chemical- or CVB3-induced upregulation of glucose-regulated protein78. Further, Irgm3 strongly inhibited the activation of both the PERK and ATF6 pathways of ER stress responses, which further led to the diminished phosphorylation of eIF2?, reduced cleavage/activation of transcription factor SREBP1 and attenuated induction of proapoptotic genes CHOP and GADD34. These data were further supported by experiments using Irgm3 knockout mouse embryonic fibroblasts, in which the ER stress induced by CVB3 was not relieved due to the lack of Irgm3 expression. In addition, the tunicamycin-triggered ER stress promoted the subsequent CVB3 ...

2011-10-10

238

Risk of malignant lymphoma in patients with inflammatory bowel diseases: A Dutch nationwide study.

Testosterone has bipotential effects on male fitness; that is, it both suppresses immune function and maintains characteristics important for reproductive success. Presumably, these effects of testosterone may be more pronounced among polygynous species because testosterone concentrations are generally higher among polygynous than monogamous males. The present study examined sex and species differences in cell-mediated immunity among four arvicoline rodents. The role of mating system and sex steroids in sex differences in immune function was examined in individually housed polygynous meadow (Microtus pennsylvanicus) and montane (M. montanus) voles and monogamous prairie (M. ochrogaster) and pine (M. pinetorum) voles in Experiment 1. No sex differences in splenocyte proliferation were observed among the four species and circulating testosterone concentrations did not correlate with immune function of individuals within each species. The contribution of social ...

1997-08-01

239

Olfactomedin 4 suppresses prostate cancer cell growth and metastasis via negative interaction with cathepsin D and SDF-1.

BACKGROUND: Immune suppressant medications such as thiopurines and anti-tumor necrosis factor agents are important for maintaining disease control in most patients with inflammatory bowel diseases (IBDs); however, their use has been associated with the development of malignant lymphoma. The purpose of this Dutch nationwide study was to estimate the relative risk of malignant lymphoma in IBD patients. METHODS: IBD patients who developed a lymphoma between 1997 and 2004 were identified using the Dutch National Database of PALGA. Data from confirmed cases were collected from individual hospitals, including data on Epstein-Barr virus (EBV). The age-adjusted 8-year incidence of malignant lymphoma in the Netherlands was retrieved from the Central Bureau of Statistics. RESULTS: Forty-two hospitals were visited and 285 matches evaluated in the total cohort of 17,834 IBD patients. Forty-four lymphomas were observed, resulting in a relative risk of 1.27 ...

2010-12-22

240

Kokanee Stock Status and Contribution of Cabinet Gorge Hatchery, Lake Pend Oreille, Idaho, 1986 Annual Progress Report.

The human olfactomedin 4 gene (OLFM4) encodes an olfactomedin-related glycoprotein. OLFM4 is normally expressed in a limited number of tissues, including the prostate, but its biological functions in prostate are largely unknown. In this study, we found that OLFM4 messenger RNA was reduced or undetectable in prostate cancer tissues and prostate cancer cell lines. To study the effects of OLFM4 on prostate cancer progression, we transfected PC-3 prostate cancer cells with OLFM4 to establish OLFM4-expressing PC-3 cell clones. The OLFM4-expressing PC-3 cell clones were found to have decreased proliferation and invasiveness compared with vector-transfected control PC-3 cells in vitro. In addition, nude mice injected with OLFM4-expressing PC-3 cells demonstrated reduced tumor growth and bone invasion and metastasis compared with mice injected with vector-transfected control cells. Mechanistic studies revealed that OLFM4 may exhibit its anticancer effects through regulating cell autophagy by ...

2011-04-05

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241

Measurements of Cabibbo Suppressed Hadronic Decay Fractions of Charmed D0 and D+ Mesons

Estimated kokanee (Oncorhynchus nerka) abundance in Lake Pend Oreille was 4.3 million during September 1986. This estimate was similar to 1985 and indicates continued suppression of the kokanee population since initial decline in the late 1960s. Atypically high survival of wild fry resulted in similar fry recruitment in 1986 as 1985, whereas hatchery-reared fry contributed only 8% to total fry recruitment as a result of low post-release survival (3%). Fry released into the Clark Fork River from Cabinet Gorge Hatchery had very low survival during emigration to Lake Pend Oreille, resulting from poor flow conditions and potentially high predation. Fry survival during emigration was twice as high during nighttime flows of 16,000 cfs than 7,800 cfs. Emigration also was faster during higher flows. Several marks were tested to differentially mark fry release groups to help determine impacts of flow and other factors on fry survival. Survival of fry ...

1987-02-01

242

Structural analysis of covalently labeled estrogen receptors by limited proteolysis and monoclonal antibody reactivity

Using data collected with the BESII detector at $e^{+}e^{-}$ storage ring Beijing Electron Positron Collider, the measurements of relative branching fractions for seven Cabibbo suppressed hadronic weak decays $D^0 \\to K^- K^+$, $\\pi^+ \\pi^-$, $K^- K^+ \\pi^+ \\pi^-$ and $\\pi^+ \\pi^+ \\pi^- \\pi^-$, $D^+ \\to \\bar{K^0} K^+$, $K^- K^+ \\pi^+$ and $\\pi^- \\pi^+ \\pi^+$ are presented.

2005-01-01

243

MHC-like molecules in some nonmammalian vertebrates can be detected by some cross-reactive xenoantisera.

The authors have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with ["3H]tamoxifen aziridine (["3H]TAZ) were treated with trypsin (T), #alpha#-chymotrypsin (C), or Staphylococcus aureus V8 protease prior to electrophoresis on sodium dodecyl sulfate gels. Fluorography revealed a distinctive ladder of ER fragments containing TAZ for each protease generated from the M/sub r/ 66,000 ER. Immunoblot detection with the primate-specific antibody D75P3#gamma# revealed that all immunoreactive fragments corresponded to TAZ-labeled fragments but that some small TAZ-labeled fragments were no longer immunoreactive. In contrast, use of the antibody H222SP#gamma# revealed a correspondence between TAZ-labeled ...

244

Dissociation of insulin receptor phosphorylation and stimulation of glucose transport in BC3H-1 myocytes

Rabbit antisera raised to human and chicken MHC molecules were used to immunoprecipitate cross-reactive molecules from biosynthetically and cell surface-labeled spleen and/or blood cells of representative vertebrate species. Five major points emerged: 1) There were many nonspecific cross-reactions using these techniques, so various criteria were developed to distinguish these from true MHC-like molecules. 2) Only very small subpopulations of immunogen-specific antibodies cross-reacted with MHC-like molecules in other nonmammalian species. These subpopulations were different for each species and even within a species, sometimes being so limited as to behave like alloantisera. This led to a very scattered pattern of true cross-reactions that sometimes failed to reflect the properties of the bulk antibody population. 3) Antisera containing antibodies to class II beta- and class I alpha-chains cross-reacted better and more ...

1990-01-01

245

Curious Results with Palladium- and Platinum-Carrying Polymers in Mass Cytometry Bioassays and an Unexpected Application as a Dead Cell Stain.

The authors have investigated insulin receptor phosphorylation in differentiated cultured BC3H-1 myocytes. As for other insulin-responsive cell systems in partially purified wheat germ agglutinin receptor preparations, insulin stimulates the phosphorylation of its own receptor (95K ..beta..-subunits) in a dose dependent manner (0-400 nM), as identified by immunoprecipitation with antiinsulin receptor antibodies and SDS-PAGE. In the same preparations they show that 12-0-tetradecanyl phorbol acetate (TPA), which in many respect ..beta..-subunits in the same dose dependent manner (0-5 ..mu..M). In addition, antiinsulin receptor antibodies (B-10) also induced phosphorylation of mimics insulin action, also induced phosphorylation of the insulin receptor and HPLC tryptic maps of the /sup 32/P-labeled ..beta..-subunit were identical to those for insulin-induced receptor phosphorylation. However, while insulin and TPA are potent stimulators of glucose ...

1986-05-01

246

Soybean Based Diets and CVD Risk Factors

We describe the synthesis of metal-chelating polymers (MCPs) with four different pendant polyaminocarboxylate ligands (EDTA, DTPA, TTHA, DOTA) and an orthogonal end-group, either a fluorescein molecule or a bismaleimide linker for antibody attachment. Polymer characterization by a combination of (1)H NMR, UV/vis absorption measurements, and thermal gravimetric analysis (TGA) indicated that each chain of the fluorescein-terminated polymers contained one dye molecule. These polymer samples were loaded with three different types of lanthanide ions as well as palladium and platinum ions. The numbers of metal atoms per chain were determined by a combination of UV/vis and conventional ICP-MS measurements. The experiments with lanthanide ions demonstrated that a net anionic charge on the polymer is important for water solubility. These experiments also showed that at least one type of lanthanide ion (La(3+)) is capable of forming a bimetallic complex with pendant DTPA ...

2011-09-28

247

Transient suppression of MLH1 allows effective single-nucleotide substitution by single-stranded DNA oligonucleotides

Hypercholesterolemia

2005-09-15

248

British Library Electronic Table of Contents (United Kingdom)

Short synthetic single-stranded oligodeoxyribonucleotides (ssODNs) can be used to introduce subtle modifications into the genome of mouse embryonic stem cells (ESCs). We have previously shown that effective application of ssODN-mediated gene targeting in ESC requires (transient) suppression of DNA mismatch repair (MMR). However, whereas transient down-regulation of the mismatch recognition protein MSH2 allowed substitution of 3 or 4 nucleotides, 1 or 2 nucleotide substitutions were still suppressed. We now demonstrate that single- or dinucleotide substitution can effectively be achieved by transient down-regulation of the downstream MMR protein MLH1. By exploiting highly specific real-time PCR, we demonstrate the feasibility of substituting a single basepair in a non-selectable gene. Howev...

2011-01-01

249

TUNABLE FIBER FABRY-PEROT FILTER FOR OPTICAL CARRIER-SUPPRESSION AND SINGLE-SIDEBAND MODULATION IN RADIO OVER FIBER LINKS

British Library Electronic Table of Contents (United Kingdom)

A novel method of simultaneous realization of optical carrier-suppression and single-sideband modulation using fiber Fabry-Perot tunable filter is presented. In order to enhance transmission performance of radio over fiber links, we use a fiber Fabry-Perot tunable filter to filter out one sideband as well as suppress the optical carrier power. The results demonstrate 20.5dB and 14.2dB improvement in the signal noise ratios when 18GHz and 10GHz microwave signals carrying 5Mbit/s quadrature-phase-shift-keyed (QPSK) format data is transmitted over 35 km single mode fiber, respectively.

2006-01-01

250

Plasma processing: a novel method to reduce the transient enhanced diffusion of boron implanted in silicon

Studies on metabolism of directly labeled {sup 99}MTc-antibody in mice

In this paper a novel method is presented, based on the use of plasma processing, to suppress the transient enhanced diffusion of boron implanted in silicon. We found for silicon samples processed with plasma and subsequently boron implanted that the anomalous diffusion of the dopant atoms at the beginning of the annealing process is almost completely suppressed. This phenomenon is interpreted in terms of capture of the ion beam generated interstitials by the dislocations induced by the plasma processing. At room temperature the dislocations are observed to grow in size after the boron implant, attesting their efficiency as trapping centres for interstitials. Moreover, varying the plasma process conditions we can establish a general relation between the presence of the trapping centres induced by the plasma processing and the suppression of the transient diffusion.

1999-01-01

251

Euglena light-harvesting chlorophyll A/B binding protein (LHCP) synthesized as an unusually large precursor

The elucidation on the metabolic products of the {sup 99}mTc-antibody conjugates may provide insights and approaches that would reduce the undesirable deposition of radioactive species in normal tissue. In this investigation, the radiolabeled species in blood, urine, bile and extracts of liver and kidney obtained at different times after the injection of a model antibody, {sup 99}mTc, into mice were analyzed with various chromatographic methods. Ninety-nine to 100% of the radioactivity in serum was associated with intact Mab 170. The radioactivity in liver homogenate extract was strictly protein-bound to either intact Mab or low molecular weight species (LMW). In kidney extracts, the majority of the radioactivity was protein bound {sup 99}mTc, with less than 8% of the activity being non- protein bound . Multiple {sup 99}mTc -containing protein and non-protein species were found in urine and bile. Evidence supporting the presence of {sup ...

1996-12-01

252

No evidence of over-reporting of SARS in mainland China

Light increased the rate of LHCP synthesis as measured by pulse-labeling with /sup 35/SO/sub 4/ and immunoprecipitation with antibody specific for Euglena LHCP. In addition to the mature LHCP, 26,000 daltons, the LHCP specific antibody immunoprecipitated large amounts of several proteins having molecular weights of approximately 100,000. On immunoblots of immunoprecipitated unlabeled protein, the antibody only detected the mature LHCP suggesting that the high molecular weight proteins are not LHCP aggregates produced during immunoprecipitation. After a 10 min pulse with /sup 35/SO/sub 4/, the 100,000 dalton proteins constituted over 80% of the immunoprecipitated material. In a subsequent chase, the radioactivity in the 100,000 dalton proteins decreased and the radioactivity in the mature LHCP increased suggesting a precursor-product relationship. After a 35 minute chase, the mature LHCP was the major radioactive protein ...

1987-04-01

253

British Library Electronic Table of Contents (United Kingdom)

Summary Objective To find out whether there was over-reporting of SARS patients in mainland China in view of the relatively low case fatality ratio in mainland China, in comparison with other affected countries and areas. Methods We searched PubMed for all SARS antibody detection papers (in English or Chinese language) using the keywords `SARS' and `antibody'. Then the resulting articles were further read through to select the SARS detection results using ELISA methods of serum samples collected at least 1 month after disease onset. A multi-level logistic regression was applied to test for possible differences in the proportions positive between locations of the study. Results A total of 48 studies were identified, including 39 from mainland China and nine from elsewhere (Hong Kong, Taiwan...

2009-01-01

254

Mesenchymal stem cell transplantation for diffuse alveolar hemorrhage in SLE

British Library Electronic Table of Contents (United Kingdom)

Background. A 19-year-old girl was diagnosed with systemic lupus erythematosus, based on findings of arthritis, malar rash, positive antinuclear antibody test and high levels of antibodies to double-stranded DNA. Two months after diagnosis, the patient presented with a sudden drop in blood hemoglobin level. Several days later, she developed bloody sputum, rapidly progressive dyspnea and hypoxemia. High-resolution CT showed diffuse alveolar infiltrates in both lung fields.Investigations. Physical examination, complete blood count, erythrocyte sedimentation rate, urinalysis, 24-h urine protein excretion, fecal occult blood test, d-dimer test, acid hemolysis test, activated partial thromboplastin time and prothrombin time, direct and indirect Coombs tests, bone marrow smear, arterial blood ga...

2010-01-01

255

In vivo chicken model for peripheral intravascular human fibrin clot detection

Follow-up of pregnant women exposed to chicken pox: an audit of relationship between level of antibody and development of chicken pox

A chicken model was prepared that provides a simple and economical method of evaluating the use of fibrin-specific monoclonal antibody 64C5 in the detection of peripheral vascular thrombi. Human fibrin was clotted in segments of a chicken's femoral artery and vein prior to intravenous injection of radioiodinated antibody 64C5. After a 3-hr perfusion time, the thrombosed and contralateral control segments of the vessels were excised and counted for radioactivity. The radiolabeled 64C5 uptake ratio of the thrombosed segment to the control segment was 5.4 +/- 1.2 (p less than 0.007) in the femoral artery, and 3.8 +/- 1.1 (p less than 0.02) in the femoral vein. This in vivo chicken model may also find application in studies of targeting agents for human fibrin.

1988-08-01

256

British Library Electronic Table of Contents (United Kingdom)

The purpose of this study was to validate through natural exposure a cut-off level of varicella zoster IgG as protective against infection with varicella zoster virus (VZV). Laboratory testing to determine VZV immune status of pregnant women exposed to varicella is recommended. Quantitative assays are now available which are sensitive and specific. More than 200 consecutive requests for screening in pregnant patients with recent varicella contacts were followed-up by questionnaire. DiaSorin LIAISON and VZV time resolved fluorescence immuno assay (VZV TRFIA) were used to measure VZV antibody level. One hundred fifty out of 209 (72%) questionnaires were returned; 14 patients developed varicella, 129 did not and seven were not known. Patients who had been given VZIG and developed varicella on...

2011-01-01

257

Antigenic characterization of phocine distemper virus causing mass mortality in 2002 and its relationship to other morbilliviruses

British Library Electronic Table of Contents (United Kingdom)

Summary The antigenic relationship between the phocine distemper virus (PDV) strain causing the epidemic in 2002 and the PDV strain of 1988, canine distemper virus from two dogs and one marten, and one measles virus strain was investigated in vivo and in vitro using monospecific polyclonal and monoclonal antibodies directed against five different proteins of canine or phocine distemper virus (N, P, M, F, H). Epitopic mapping revealed no difference between the PDV strains causing the epidemics in 1988 or 2002. However, the use of these antibodies allowed discrimination between different morbilliviruses including a vaccine strain of canine distemper virus. The major differences among the investigated morbilliviruses were found in the H protein.

2007-01-01

258

Adalimumab treatment in Crohn's disease does not induce early changes in regulatory T cells

British Library Electronic Table of Contents (United Kingdom)

Abstract Objective. Anti-TNF-a antibodies has been suggested to modulate regulatory T cell (Treg) percentages in rheumatoid arthritis, but results from studies of Crohn's disease (CD) are conflicting. We investigated dynamic changes of circulating Tregs in CD during treatment with the anti-TNF-a-antibody adalimumab (Humira, Abbott Laboratories A/S, Emdrupvej 28C, DK-2100 Copenhagen). Material and methods. Blood samples from 26 CD patients were analysed using flow cytometry before and 1 and 26 weeks after initiation of adalimumab treatment to determine the percentage of Tregs among CD4++ T cells. Results. In spite of a significant decline in disease activity scores and biochemical markers of inflammation, during the first week of treatment, we did not observe early modulating effects of ada...

2011-01-01

259

Launching proton-dominated jets from accreting Kerr black holes: the case of M87

Update on the Role of Substantia Nigra Pars Reticulata in the Regulation of Seizures

A general relativistic model for the formation and acceleration of lowmass-loaded jets from systems containing accreting black holes is presented. The model is based on previous numerical results and theoretical studies in the Newtonian regime, but modified to include the effects of space-time curvature in the vicinity of the event horizon of a spinning black hole. It is argued that the boundary layer between the Keplerian accretion disk and the event horizon is best suited for the formation and acceleration of the accretion-powered jets in active galactic nuclei and micro-quasars. The model presented here is based on matching the solutions of three different regions: i- a weakly magnetized Keplerian accretion disk in the outer part, where the transport of angular momentum is mediated through the magentorotational instability, ii- a strongly magnetized, advection-dominated and turbulent-free boundary layer (BL) between the outer cold accretion disk and the event horizon and where the ...

2011-07-01

260

The Detection of Pressure Fluctuations, Sonic Audition, Is the Dominant Mode of Dipole-Source Detection in Goldfish (Carassius auratus)

The substantia nigra pars reticulata (SNR) represents an endogenous seizure suppressing system, which may be targeted to develop treatments for generalized or multifocal epilepsies. This review summarizes...Full Text Available

2006-05-01

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261

Suppressive effect of azithromycin on Plasmodium berghei mosquito stage development and apicoplast replication

Behavioral detection of a low-frequency (40 Hz) vibratory dipole at source distances of 1.5–24 cm was measured by classically conditioned respiratory suppression in goldfish (Carassius...Full Text Available

2009-04-01

262

Suppression of Allene Oxide Cyclase in Hairy Roots of Medicago truncatula Reduces Jasmonate Levels and the Degree of Mycorrhization with Glomus intraradices1[w

BackgroundAzithromycin (AZM) is a macrolide antibiotic that displays an excellent safety profile even in children and pregnant women and has been shown to have anti-malarial activity...Full Text Available

263

Suppression by IgA of IgG-mediated phagocytosis by human polymorphonuclear leucocytes

During the symbiotic interaction between Medicago truncatula and the arbuscular mycorrhizal (AM) fungus Glomus intraradices, an endogenous increase in jasmonic acid...Full Text Available

2005-11-01

264

Studies on Inhibition of Intestinal Absorption of Radioactive Strontium

IgA from normal human serum, a myeloma IgA and human colostral IgA were found to inhibit the IgG-dependent phagocytosis of Candida albicans (CA) blastospores by polymorphonuclear leucocytes...Full Text Available

1978-12-01

265

Regulatory T Cells in Many Flavors Control Asthma

A method is reported which permits selective suppression of absorption of radioactive strontium from ingested food material, permitting the calcium to be available to the body. Studies were carried...Full Text Available

1964-08-08

266

Regulation of Mixed Lineage Kinase 3 is Required for Neurofibromatosis-2-Mediated Growth Suppression in Human Cancer

That regulatory T cells (Tregs) have a crucial role in controlling allergic diseases such as asthma is now undisputed. The cytokines most commonly implicated in Treg-mediated suppression of...Full Text Available

2010-05-01

267

RU486 did not exacerbate cytokine release in mice challenged with LPS nor in db/db mice

The Neurofibromatosis-2 (NF2) tumor suppressor merlin negatively regulates cell proliferation in numerous cell types. We have previously shown that the NF2...Full Text Available

2011-02-17

268

Noise suppression in scatter correction for cone-beam CT

BackgroundGlucocorticoids down-regulate cytokine synthesis and suppress inflammatory responses. The glucocorticoid receptor (GR) antagonist RU486 may exacerbate the inflammatory...Full Text Available

269

Interactions of Top-Down and Bottom-Up Mechanisms in Human Visual Cortex

Scatter correction is crucial to the quality of reconstructed images in x-ray cone-beam computed tomography (CBCT). Most of existing scatter correction methods assume smooth scatter distributions. The...Full Text Available

2009-03-01

270

Integrative Analysis of Epigenetic Modulation in Melanoma Cell Response to Decitabine: Clinical Implications

Multiple stimuli present in the visual field at the same time compete for neural representation by mutually suppressing their evoked activity throughout visual cortex, providing a neural correlate...Full Text Available

2011-01-12

271

Immune activation and IL-12 production during acute/early HIV infection in the absence and presence of highly active, antiretroviral therapy

Decitabine, an epigenetic modifier that reactivates genes otherwise suppressed by DNA promoter methylation, is effective for some, but not all cancer patients, especially those with solid tumors. It...Full Text Available

272

Expression of Fc receptors is suppressed in alveolar macrophages from patients with sarcoidosis.

Suppressed IL-12 production and maladaptive immune activation, both of which are ameliorated by successful highly active antiretroviral therapy (HAART), are thought to play important roles in the immunopathogenesis...Full Text Available

2008-12-01

273

Evaluation of a Superconducting Fault Current Limiter Model for Electric Power System

To study the expression of Fc receptors in human alveolar macrophages (AM), the cells were collected from 12 healthy controls and 22 patients with sarcoidosis and the activity involved in binding to...Full Text Available

1988-09-01

274

E2f binding-deficient Rb1 protein suppresses prostate tumor progression in vivo

Fault current limiters (FCLs) are devices for to suppress current in electric power system. The FCL is extensively expected to suppress fault current, particularly required for trunk power systems heavily connected high-voltage transmission lines, such as 500 kV class power system. For this reason, FCLs based on various principles of operation have been developed. Most of the FCLs under development which employ superconductors are based on the quenching phenomenon peculiar to the superconducting state, that is, making use of a rapid transition to normal conducting state, an electrical resistance appears, and this resistance is used to suppress fault currents. However, this elevation of resistance, although increasing the fault current suppression effect, also tend to raise the overvoltage occurring FCL, the ascertainment of the overvoltage is important as regards the protection of the FCL as well as ...

2003-01-01

275

Dynamic load in suppression pool during BWR main steam safety relief valve actuation

Mutational inactivation of the RB1 tumor suppressor gene initiates retinoblastoma and other human cancers. RB1 protein (pRb) restrains cell proliferation by binding...Full Text Available

2011-01-11

276

Association of Protein Phosphatase 1?₁ with Spinophilin Suppresses Phosphatase Activity in a Parkinson Disease Model*

BWRs are so designed that the exhaust steam from main steam safety relief valves is led to pressure suppression pools, and the steam is condensed in pool water, but at this time, dynamic load seems to arise in the pool water. In Tokai No. 2 Power Station, a Mark-2 containment vessel was adopted to improve the reliability as much as possible and to obtain the design with margin. In this report, the result of actual machine test in Tokai No. 2 Power Station and the method of reducing the load are described. When a relief valve works, the discharge of water in exhaust pipes into a suppression pool, the exhaust of air in exhaust pipes and repeated expansion and contraction of bubbles in pool water, and the exhaust of steam and condensation occur. As for the construction of the suppression pool in Tokai No. 2 Power Station, cross-shaped quencher and the structure with jet deflector were installed. The test plan and the test ...

1979-01-01

277

Antimalarial activities and subacute toxicity of RC-12, a 4-amino-substituted pyrocatechol.

Sustained nigrostriatal dopamine depletion increases the serine/threonine phosphorylation of multiple striatal proteins that play a role in corticostriatal synaptic plasticity, including Thr²⁸⁶...Full Text Available

2008-05-23

278

Amber suppression in Escherichia coli by unusual mitochondria-like?transfer?RNAs

RC-12 [1,2-dimethoxy-4-(bis-diethylaminoethyl)-amino-5-bromobenzene] was evaluated for prophylactic, radical curative, and suppressive activities against infections with Plasmodium cynomolgi and subacute...Full Text Available

1985-11-01

279

Aggregation Substance Promotes Adherence, Phagocytosis, and Intracellular Survival of Enterococcus faecalis within Human Macrophages and Suppresses Respiratory Burst

The “cloverleaf” base-pairing pattern was established as the structural paradigm of active tRNA species some 30 years ago. Nevertheless, this pattern does not accommodate the folding...Full Text Available

1998-02-17

280

Abscisic Acid Inhibition of Radicle Emergence But Not Seedling Growth Is Suppressed by Sugars1

The aggregation substance (AS) of Enterococcus faecalis, encoded on sex pheromone plasmids, is a surface-bound glycoprotein that mediates aggregation between bacteria thereby facilitating...Full Text Available

2000-09-01

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281

A fuel treatment reduces potential fire severity and increases suppression efficiency in a Sierran mixed conifer forest

Low concentrations of sugars altered the sensitivity of seed germination to inhibition by exogenous abscisic acid (ABA). Germination of wild-type and ABA-insensitive (abi) Arabidopsis...Full Text Available

2000-04-01

282

A cytochrome P450 terpenoid hydroxylase linked to the suppression of insect juvenile hormone synthesis

Fuel treatments are being widely implemented on public and private lands across the western U.S. While scientists and managers have an understanding of how ... ...

283

A Suppression Subtractive Hybridization Approach Reveals Niche-Specific Genes That May Be Involved in Predator Avoidance in Marine Synechococcus Isolates

A cDNA encoding a cytochrome P450 enzyme was isolated from a cDNA library of the corpora allata (CA) from reproductively active Diploptera punctata cockroaches. This P450 from the endocrine...Full Text Available

1998-10-27

284

Continuum background suppression using various selectors

Picocyanobacteria of the genus Synechococcus are important contributors to marine primary production and are ubiquitous in the world's oceans. This genus is genetically diverse, and...Full Text Available

2006-04-01

285

Extraction of Primary Factors Influencing Dam Operation Using Factor Analysis

Continuum events represent an eminent source of background in any e+e- experiment. As these have a higher branching ratio than BB-bar events (at BaBar this ratio is estimated to about 3.5) or ?+?- events, efficient continuum background suppression is essential in many analyses. Using Artificial Neural Networks and the Nearest Neighbor Method we developed several selectors which, based only on the global event shape variables, efficiently tag BB-bar events and ?+?- events against the continuum background. These selectors could then be combined with the channel specific information in various types of analyses. The study was done using a parametric Monte Carlo.

1999-10-04

286

Application of dose factors for decay chains

Full Text Available

2007-10-01

287

Development of radioimmunometric assays and kits for non-clinical applications. Proceedings of a final research coordination meeting

... dose rates inhalation iodine 134 krypton 88 nuclear decay quality factor

288

The incidence of spontaneously occurring antiliver antibodies in New Zealand Black mice

Immunoassays are versatile analytical techniques that had a leading role in various clinical applications, during the last four decades. The studies carried out by Rosalyn Yalow, Solomon Berson and Roger Ekins in the 1960s gave a breakthrough in the development of this novel analytical method. Sensitivity up to femtomolar concentrations, high specificity and universal application to different classes of molecular species made immunoassay a very useful tool in analytical investigation. The expertise acquired by immunochemists in producing antibodies against any antigen and the ability of radiochemists in labeling the antigens with "1"2"5I without affecting the active site are the two main factors responsible for the above development. There are concerns about the safety and health of humans due to the high-level contamination of environment by pesticides, industrial compounds and metals, anabolic steroids in milk and meat products, and presence ...

2004-12-06

289

Staphylococcus aureus Enterotoxin D Is Secreted in Milk and Stimulates Specific Antibody Responses in Cows in the Course of Experimental Intramammary Infection

The sera of NZB/BL mice have been found to be active in the complement fixation test against isogeneic liver antigens to a higher titre than the sera of CBA, C57BL and DBA/2 mice. Six sera from NZB...Full Text Available

1969-04-01

290

Presence of antibodies to a putatively immunosuppressive part of human immunodeficiency virus (HIV) envelope glycoprotein gp41 is strongly associated with health among HIV-positive subjects.

An enterotoxin D (SED)-producing strain of Staphylococcus aureus was used to infect one mammary gland of each of 17 lactating dairy cows. All glands became infected and shed bacteria...Full Text Available

2006-06-01

291

Murine antibody response to oral infection with live aroA recombinant Salmonella dublin vaccine strains expressing filamentous hemagglutinin antigen from Bordetella pertussis.

The IgG response to gp41 (envelope glycoprotein of Mr 41,000) of the human immunodeficiency virus (HIV) was studied with eight synthetic peptides derived from three different regions of the protein....Full Text Available

1988-07-01

292

Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice.

Two plasmids which express either nearly intact or truncated filamentous hemagglutinin (FHA) from Bordetella pertussis and which are marked with a tetracycline resistance (Tcr) gene were transformed into Salmonella dublin SL1438, an aroA deletion mutant intended for use as an attenuated oral vaccine against salmonellosis. These S. dublin recombinants, when fed to mice, induced serum immunoglobulin, immunoglobulin M (IgM), and sometimes IgA antibody responses to FHA and S. dublin. In addition, IgA antibodies against FHA were found in gut wash fluids. S. dublin carrying pDB2300, a multicopy plasmid encoding truncated FHA protein, induced a better antibody response than did S. dublin carrying pDB2000, a low-copy-number plasmid encoding full-sized FHA. Administration of tetracycline to mice enhanced the stability of recombinant plasmids, and tetracycline-treated mice developed higher anti-FHA titers. Although neither strain ...

1990-08-01

293

Monoclonal antibodies define genus-specific, species-specific, and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): specific immunodetection and purification of chlamydial hsp60.

We have recently shown that viable Borrelia burgdorferi organisms induce a chronic infection associated with arthritis and carditis in severe combined immunodeficiency (scid) mice but not in immunocompetent...Full Text Available

1990-05-01

294

Lyme myocarditis diagnosed by indium-111-antimyosin antibody scintigraphy

Ocular and urogenital tract infections with Chlamydia trachomatis can progress to chronic inflammatory diseases that produce blindness and tubal infertility. The pathophysiology of these chronic disease...Full Text Available

1992-06-01

295

Kidney-specific allo- and autoantibodies in the alloantibody response to rat kidney: the use of kidney homogenate as a target for serological analysis.

We report a new case of Lyme disease with cardiac manifestations, which has been possible to follow during the long period of 12 years. We have detected the usual ECG abnormalities, and concentric hypertrophic myocardiopathy, by echocardiography. The acute myocarditis was demonstrated by /sup 111/In-antimyosin scintigraphy, which showed global myocardial uptake of the tracer, constituting the first report, to our knowledge, of Lyme myocarditis diagnosed by this method.

1989-06-01

296

Induction of Antibody Responses to African Horse Sickness Virus (AHSV) in Ponies after Vaccination with Recombinant Modified Vaccinia Ankara (MVA)

LEW anti-DA kidney and DA anti-LEW kidney sera were assayed using an indirect 125I anti-immunoglobulin-binding assay with kidney homogenate as target. This allowed the full spectrum of antibodies to...Full Text Available

1980-04-01

297

Homocytotropic antibodies (IgE) to Neisseria gonorrhoeae in the rat and a cross-reactivity of heterologous gonococcal strains.

BackgroundAfrican horse sickness virus (AHSV) causes a non-contagious, infectious disease in equids, with mortality rates that can exceed 90% in susceptible horse populations....Full Text Available

298

Glutamine-linked and Non-consensus Asparagine-linked Oligosaccharides Present in Human Recombinant Antibodies Define Novel Protein Glycosylation Motifs

Outbred Wistar rats were immunised with a single intraperitoneal injection of a mixture of 30 mg of A1(OH)3 and 100microng of gonococcal zeolite antigen (ZA). Ten days after immunisation, ZA prepared...Full Text Available

1977-04-01

299

False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV

We report the presence of oligosaccharide structures on a glutamine residue present in the V_L domain sequence of a recombinant human IgG2 molecule. Residue Gln-106, present in the QGT sequence...Full Text Available

2010-05-21

300

Expression of a functional VEGFR-1 in tumor cells is a major determinant of anti-PlGF antibodies efficacy

To evaluate the reactivity of the recombinant proteins expressed in Escherichia coli strain BL21(DE3), a Western blot assay was performed by using a panel of 78 serum samples obtained,...Full Text Available

2006-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

Enzymic synthesis of 1-O-(indol-3-ylacetyl)-beta-D-glucose. Purification of the enzyme from Zea mays, and preparation of antibodies to the enzyme.

PlGF, one of the ligands for VEGFR-1, has been implicated in tumor angiogenesis. However, more recent studies indicate that genetic or pharmacological inhibition of PlGF signaling does not result in...Full Text Available

2011-07-12

302

Differential Specificity and Immunogenicity of Adenovirus Type 5 Neutralizing Antibodies Elicited by Natural Infection or Immunization?

The enzyme indol-3-ylacetylglucose synthase (UDP-glucose:indol-3-ylacetate beta-D-glucosyltransferase) catalyses the reaction: [formula: see text] This is the first step in the series of reactions leading...Full Text Available

1991-10-15

303

Developmentally regulated expression of a 78 kDa erythroblast membrane glycoprotein immunologically related to the platelet thrombospondin receptor.

A recent clinical trial of a T-cell-based AIDS vaccine delivered with recombinant adenovirus type 5 (rAd5) vectors showed no efficacy in lowering viral load and was associated with increased risk of...Full Text Available

2010-01-01

304

A Single Domain Llama Antibody Potently Inhibits the Enzymatic Activity of Botulinum Neurotoxin by Binding to the Non-Catalytic Alpha-Exosite Binding Region

We have previously described a monoclonal antibody (FA6-152), obtained by immunizing mice with fetal human erythrocytes [Edelman, Vinci, Villeval, Vainchenker, Henri, Miglierina, Rouger, Reviron, Breton-Gorius,...Full Text Available

1989-09-15

305

??⁺ T-Lymphocyte Cytotoxicity against Envelope-Expressing Target Cells Is Unique to the Alymphocytic State of Bovine Leukemia Virus Infection in the Natural Host

Ingestion or inhalation of botulinum neurotoxin (BoNT) results in botulism, a severe and frequently fatal disease. Current treatments rely on antitoxins, which while effective cannot reverse...Full Text Available

2010-04-09

306

Possible health effects of working with VDUs.

Bovine leukemia virus (BLV) is a complex B-lymphotrophic retrovirus of cattle and the causative agent of enzootic bovine leukosis. Serum antibody in infected animals does not correlate with protection...Full Text Available

2000-09-01

307

Human Factors in Network Security

A summary of the effects presented here is given in the table. The designation "factor present" implies that there is knowledge (from human or animal studies, or both) of a specific factor(s) present...Full Text Available

1989-04-01

308

Structure-activity relationships of anthraquinones on the suppression of DNA-binding activity of the aryl hydrocarbon receptor induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin.

... TITLE (Indlude Security Classifkcation) Human Factors in Network Security 12. ... FIELD GROUP SUBGROUP Human Factors, Network, Security 19. ...

1991-03-21

309

Neurologic factors in female sexual function and dysfunction.

Anthraquinones are widely present in plant kingdom, and clinically used as laxatives. Environmental contaminants, dioxins, develop various adverse effects through transformation of a cytosolic aryl hydrocarbon receptor (AhR). We investigated the effects of 18 anthraquinones and 7 of their structurally related compounds on transformation of the AhR estimated by its DNA-binding activity in the cell-free system. 1,4-Dihydroxyanthraquinone (quinizarin), 1,5-dihydroxyanthraquinone (anthrarufin), 1,8-dihydroxyanthraquinone (danthron), and 5-hydroxy-1,4-naphthoquinone (juglone) strongly suppressed DNA-binding activity of the AhR induced by 0.1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), with their IC(50) values around 1 muM. On the other hand, anthraquinone, 2,6-dihydroxyanthraquinone (anthraflavic acid), and 2-hydroxy-1,4-naphthalendione (lawsone) showed moderate effects. Quantitative structure-activity relationships analysis demonstrated that hydroxyl groups at C1 or ...

2009-03-01

310

Lack of association between polymorphisms in C4b-binding protein and atypical haemolytic uraemic syndrome in the Spanish population

Full Text Available

2010-07-01

311

Estrogen and brain-derived neurotrophic factor (BDNF) in hippocampus: complexity of steroid hormone-growth factor interactions in the adult CNS.

Dysregulation of the alternative pathway of complement activation, caused by mutations or polymorphisms in the genes encoding factor H, membrane co-factor protein, factor I or factor B, is associated...Full Text Available

2009-01-01

312

Disulfide Bonds and the Quaternary Structure of Factor VIII/von Willebrand Factor

In the CNS, there are widespread and diverse interactions between growth factors and estrogen. Here we examine the interactions of estrogen and brain-derived neurotrophic factor (BDNF), two...Full Text Available

2006-12-01

313

A Study on Success Factors for Entrepreneurs in IT Ventures

Human Factor VIII/von Willebrand factor, purified by calcium citrate-cellulose chromatography and 4% agarose gel filtration was subjected to sodium dodecyl sulfate gel electrophoresis on gels containing...Full Text Available

1978-09-01

314

Study of initial stage in coal liquefaction. Increase in oil yield with suppression of retrogressive reaction during initial stage; Ekika hanno no shoki katei ni kansuru kenkyu. 1.

Full Text Available

2010-07-01

315

Phosphorylation of proteins in Clostridium thermohydrosulfuricum

For the coal liquefaction, improvement of liquefaction conditions and increase of liquefied oil yield are expected by suppressing the recombination through rapid stabilization of pyrolytic radicals which are formed at the initial stage of liquefaction. Two-stage liquefaction combining prethermal treatment and liquefaction was performed under various conditions, to investigate the effects of reaction conditions on the yields and properties of products as well as to increase liquefied oil yield. Consequently, it was found that the catalyst contributes greatly to the hydrogen transfer to coal at the prethermal treatment. High yield of n-hexane soluble fraction with products having low condensation degree could be obtained by combining the prethermal treatment in the presence of hydrogen and catalyst with the concentration of slurry after the treatment. This was considered to be caused by the synergetic effect between the improvement of liquefaction by ...

1996-10-28

316

Morusin induces apoptosis and suppresses NF-#kappa#B activity in human colorectal cancer HT-29 cells

Cell extracts of the thermophile Clostridium thermohydrosulfuricum catalyzed the phosphorylation by (..gamma..-/sup 32/P)ATP of several endogenous proteins with M/sub r/s between 13,000 and 100,000. Serine and tyrosine were the main acceptors. Distinct substrate proteins were found in the soluble (e.g., proteins p66, p63, and p53 of M/sub r/s 66,000, 63,000, and 53,000, respectively) and particulate (p76 and p30) fractions, both of which contained protein kinase and phosphatase activity. The soluble fraction suppressed the phosphorylation of particulate proteins and contained a protein kinase inhibitor. Phosphorylation of p53 was promoted by 10..mu..M fructose 1,6-bisphosphate or glucose 1,6-bisphosphate and suppressed by hexose monophosphates, whereas p30 and p13 were suppressed by 5 ..mu..M brain (but not spinach) calmodulin. Polyamines, including the odd polyamines characteristic of thermophiles, modulated the labeling ...

1986-02-01

317

Modeling the suppression of boron transient enhanced diffusion in silicon by substitutional carbon incorporation

Morusin is a pure compound isolated from root bark of Morusaustralis (Moraceae). In this study, we demonstrated that morusin significantly inhibited the growth and clonogenicity of human colorectal cancer HT-29 cells. Apoptosis induced by morusin was characterized by accumulation of cells at the sub-G_1 phase, fragmentation of DNA, and condensation of chromatin. Morusin also inhibited the phosphorylation of IKK-#alpha#, IKK-#beta# and I#kappa#B-#alpha#, increased expression of I#kappa#B-#alpha#, and suppressed nuclear translocation of NF-#kappa#B and its DNA binding activity. Dephosphorylation of NF-#kappa#B upstream regulators PI3K, Akt and PDK1 was also displayed. In addition, activation of caspase-8, change of mitochondrial membrane potential, release of cytochrome c and Smac/DIABLO, and activation of caspase-9 and -3 were observed at the early time point. Downregulation in the expression of Ku70 and XIAP was exhibited afterward. Caspase-8 or wide-ranging ...

2008-07-18

318

Modeling the suppression of boron transient enhanced diffusion in silicon by substitutional carbon incorporation

Recent work has indicated that the suppression of boron transient enhanced diffusion (TED) in carbon-rich Si is caused by nonequilibrium Si point defect concentrations, specifically the undersaturation of Si self-interstitials, that result from the coupled out-diffusion of carbon interstitials via the kick-out and Frank--Turnbull reactions. This study of boron TED reduction in Si{sub 1-x-y}Ge{sub x}C{sub y} during 750{sup o}C inert anneals has revealed that the use of an additional reaction that further reduces the Si self-interstitial concentration is necessary to describe accurately the time evolved diffusion behavior of boron. In this article, we present a comprehensive model which includes {l_brace}311{r_brace} defects, boron-interstitial clusters, a carbon kick-out reaction, a carbon Frank--Turnbull reaction, and a carbon interstitial-carbon substitutional (C{sub i}C{sub s}) pairing reaction that successfully simulates carbon suppression ...

2001-08-15

319

Effect of Glycoletherdiaminetetraacetic acid on corrosion inhibition of stainless steel. Glycoleetherdiamin shisakusan ni yoru stainless ko no fushoku yokusei koka

Recent work has indicated that the suppression of boron transient enhanced diffusion (TED) in carbon-rich Si is caused by nonequilibrium Si point defect concentrations, specifically the undersaturation of Si self-interstitials, that result from the coupled out-diffusion of carbon interstitials via the kick-out and Frank--Turnbull reactions. This study of boron TED reduction in Si_1_-_x_-_yGe_xC_y during 750"oC inert anneals has revealed that the use of an additional reaction that further reduces the Si self-interstitial concentration is necessary to describe accurately the time evolved diffusion behavior of boron. In this article, we present a comprehensive model which includes #left brace#311#right brace# defects, boron-interstitial clusters, a carbon kick-out reaction, a carbon Frank--Turnbull reaction, and a carbon interstitial-carbon substitutional (C_iC_s) pairing reaction that successfully simulates carbon suppression of boron TED at ...

2001-08-15

320

Review of JT-60U experimental results from February to October, 1999

An analysis was made on corrosion suppression mechanism of glycoletherdiaminetetraacetic acid (GEDTA) film made from GEDTA on stainless steel, and its corrosion inhibition effect. Results of analysis using IR spectrum, IR-ATR spectrum, {sup 1} HNMR spectrum, and ESCA spectrum indicated the deposition of the GEDTA film is a chemical deposition. In polarization tests at pH = 5 and 7, both polarization curves shifted to the constant current density side, showing suppression effect in reactions at both pH. When the corrosion environment is made severer, such as to pH = 2 and 4, the corrosion suppression effect of GEDTA became more distinctive. A ferroxyl test identified very little difference between untreated and treated materials. In a chemical pitting test, because oxidant and corrosive anion coexist in the test solution, and GEDTA is stable against corrosive anion Cl {sup {minus}}, the film is unstable against the oxidant. ...

1990-12-20

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321

Comparison of thermochemically calculated and measured dioxin contents in the off-gas of a sinter plant

In 1999, the plasma parameters of reversed shear (RS) plasmas had been extended in 1) DT-equivalent fusion power gain Q_D_T"e"q - 0.5 (n_D(0)#tau#_ET_i(0) - 4x10"2"0 m"-"3#centre dot#keV#centre dot#s) for 0.8 s and 2) full non-inductive current drive with 80% of the bootstrap current fraction. Physics of the internal transport barriers (ITBs) in RS plasmas, including the energy transport and the formation of ITB, were extensively studied. A nearly full current drive (92% non-inductively) was obtained with negative ion based neutral beam (NNB) injection (360 keV, 3.4 MW) in a high #beta#_p H-mode plasma (I_p=1.5 MA, B_T=3.7 T, q_9_5=4.2) with high plasma performance (#beta#_N=2.4 and H_8_9=2.56). Rise in the central electron temperature (T_e - 9 keV) resulted in the current drive efficiency #eta#_C_D of NNB reached 1.3x10"1"9 A/W/m"2, the highest for the neutral beam current drive. As for the H-mode plasmas, decrease in the pedestal ion temperature due to strong gas was found cause ...

1994-06-01

322

Suppression of transient enhanced diffusion following {ital in} {ital situ} photoexcitation during boron ion implantation

Polychlorinated dibenzo-p-dioxins and dibenzo-furans form a family of more than 200 compounds which are relatively stable in the biosphere and tend to accumulate in the human body. The tetra- to hexa-chlorinated dioxins and furans are considered highly toxic. To facilitate the assessment of the total toxicity of dioxin and furan mixtures, the estimated toxic effects of the individual compounds relative to the 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) were introduced as Toxic Equivalent Factors which yield, when multiplied with the respective concentrations, the Toxic Equivalent (TE) of the mixture. Toxic dioxins and furans are unintentionally formed in a number of industrial combustion processes such as waste incineration and iron ore sintering, in the chemical industry and in household heating. To keep the emissions as low as possible, off-gas clearing systems for the collection of dioxins and furans are increasingly prescribed by the authorities. In addition, ...

1997-12-31

323

Suppression of transient enhanced diffusion following in situ photoexcitation during boron ion implantation

The effect of {ital in} {ital situ} photoexcitation during boron ion implantation on subsequent transient enhanced B diffusion in Si has been investigated. Photoexcitation using a mercury arc lamp was performed during B{sup +} implantation at 35 keV for a dose of 5{times}10{sup 14} cm{sup {minus}2} at 177 K. A reduction in the electrical activation dip, i.e., reverse annealing effect, in the temperature range 550--700 {degree}C was observed. Also, the transient enhanced diffusion of B, measured using SIMS following 800 {degree}C, 30 min annealing, was suppressed. Both effects demonstrate that the creation of self-interstitials during the implantation process is significantly reduced. {copyright} {ital 1995} {ital American} {ital Institute} {ital of} {ital Physics}.

1995-10-09

324

Superconductivity on the threshold of magnetism in CePd{sub 2}Si{sub 2} and CeIn{sub 3}

The effect of in situ photoexcitation during boron ion implantation on subsequent transient enhanced B diffusion in Si has been investigated. Photoexcitation using a mercury arc lamp was performed during B"+ implantation at 35 keV for a dose of 5x10"1"4 cm"-"2 at 177 K. A reduction in the electrical activation dip, i.e., reverse annealing effect, in the temperature range 550--700 degree C was observed. Also, the transient enhanced diffusion of B, measured using SIMS following 800 degree C, 30 min annealing, was suppressed. Both effects demonstrate that the creation of self-interstitials during the implantation process is significantly reduced. copyright 1995 American Institute of Physics.

325

Superconductivity on the threshold of magnetism in CePd_2Si_2 and CeIn_3

The magnetic ordering temperature of some rare-earth-based heavy-fermion compounds is strongly pressure dependent and can be completely suppressed at a critical pressure, p{sub c}, making way for novel correlated electron states close to this quantum critical point. We have studied the clean heavy-fermion antiferromagnets CePd{sub 2}Si{sub 2} and CeIn{sub 3} in a series of resistivity measurements at high pressures up to 3.2 GPa and down to temperatures in the mK region. In both materials, superconductivity appears in a small window of a few tenths of a GPa on either side of p{sub c}. We present detailed measurements of the superconducting and magnetic temperature-pressure phase diagram, which indicate that superconductivity in these materials is enhanced, rather than suppressed, by the closeness to magnetic order. (author)

2001-03-26

326

Status report on the fusion breeder

The magnetic ordering temperature of some rare-earth-based heavy-fermion compounds is strongly pressure dependent and can be completely suppressed at a critical pressure, p_c, making way for novel correlated electron states close to this quantum critical point. We have studied the clean heavy-fermion antiferromagnets CePd_2Si_2 and CeIn_3 in a series of resistivity measurements at high pressures up to 3.2 GPa and down to temperatures in the mK region. In both materials, superconductivity appears in a small window of a few tenths of a GPa on either side of p_c. We present detailed measurements of the superconducting and magnetic temperature-pressure phase diagram, which indicate that superconductivity in these materials is enhanced, rather than suppressed, by the closeness to magnetic order. (author)

2001-03-26

327

Quercetin-induced downregulation of phospholipase D1 inhibits proliferation and invasion in U87 glioma cells

The rationale for hybrid fusion-fission reactors is the production of fissile fuel for fission reactors. A new class of reactor, the fission-suppressed hybrid promises unusually good safety features as well as the ability to support 25 light-water reactors of the same nuclear power rating, or even more high-conversion-ratio reactors such as the heavy-water type. One 4000-MW nuclear hybrid can produce 7200 kg of /sup 233/U per year. To obtain good economics, injector efficiency times plasma gain (eta/sub i/Q) should be greater than 2, the wall load should be greater than 1 MW m/sup -2/, and the hybrid should cost less than 6 times the cost of a light-water reactor. Introduction rates for the fission-suppressed hybrid are unusually rapid.

1980-12-12

328

British Library Electronic Table of Contents (United Kingdom)

Phospholipase D (PLD) has been recognized as a regulator of cell proliferation and tumorigenesis, but little is known about the molecules regulating PLD expression. Thus, the identification of small molecules inhibiting PLD expression would be an important advance in PLD-mediated physiology. Quercetin, a ubiquitous bioactive flavonoid, is known to inhibit proliferation and induce apoptosis in a variety of cancer cells. In the present study, we examined the effect of quercetin on the expression of PLD in U87 glioma cells. Quercetin significantly suppressed the expression of PLD1 at the transcriptional level. Moreover, quercetin abolished the protein expression of PLD1 in a time and dose-dependent manner, as well as inhibited PLD activity. Quercetin suppressed NFkB-induced PLD1 expression vi...

2011-01-01

329

Optimized pulse sequences for the suppression of decoherence in quantum information

Method of feeding a coolant into a reactor

The dynamical decoupling (DD) aims at suppressing the decoherence by means of coherent control pulses. Even if devices exist where instantaneous pulses are an adequate approximation, experimentally a finite duration #tau#_p and a bounded amplitude are inevitable. They are the cause of additional errors which can be corrected by designing the pulse shape appropriately. The new pulse has the overall effect of an ideal, instantaneous pulse with the advantage of decoupling the spin (or qubit) from the bath up to the order O(#tau#_p"3). The limitation of the no-go theorem for #pi# pulses is avoided. Hence, the Uhrig sequence (UDD), originally thought for ideal #pi# pulses, works also for bounded control Hamiltonians. Numerical simulations show that concatenated sequences of real pulses are effective against general decoherence.

2010-03-21

330

Interference Mitigation Using Uplink Power Control for Two-Tier Femtocell Networks

Object: To suppress a quantity of impurities in a coolant fed into a reactor vessel. Structure: The concentration of oxygen in a coolant flowing from a condensation desalting instrument into a feed and condensation piping is measured by an oxygen-concentration detector to feed its signal to an adjusting instrument. A degree of opening of an oxygen flow control valve to maintain the concentration of oxygen in the cooling water flowing within the pipe in the range from about 10 to about 200 ppb. Also, the concentration of oxygen in the cooling water fed to the desalting instrument is maintained at a level less than 2 ppb. Thereby, the total amount of iron flown into the vessel can be suppressed to a fine amount such as less than about 1 ppb. (Kawakami, Y.).

331

High thermal load receiving heat plate

This paper proposes two interference mitigation strategies that adjust the maximum transmit power of femtocell users to suppress the cross-tier interference at a macrocell base station (BS). The open-loop and the closed-loop control suppress the cross-tier interference less than a fixed threshold and an adaptive threshold based on the noise and interference (NI) level at the macrocell BS, respectively. Simulation results show that both schemes effectively compensate the uplink throughput degradation of the macrocell BS due to the cross-tier interference and that the closed-loop control provides better femtocell throughput than the open-loop control at a minimal cost of macrocell throughput.

2009-01-01

332

First measurement of ADS parameters using B- -> D0K- decays in hadron collisions

The present invention concerns a high thermal load heat receiving plate such as a divertor plate of a thermonuclear device. The high thermal load heat receiving plate of the present invention has a cooling performance capable of suppressing the temperature of an armour tile to less than a threshold value of the material against high thermal loads applied from plasmas. Spiral polygonal pipes are inserted in cooling pipes at a portion receiving high thermal loads in the high temperature load heat receiving plate of the present invention. Both ends of the polygonal pipes are sealed by lids. An area of the flow channel in the cooling pipes is thus reduced. Heat conductivity on the cooling surface of the cooling pipes is increased in the high thermal load heat receiving plate having such a structure. Accordingly, temperature elevation of the armour tile can be suppressed. (I.S.).

1993-09-28

333

Emergency core cooling system

Measurements of branching fractions and CP-asymmetries of B^{-} \\rightarrow D^{0}K^{-} modes allow a theoretically-clean extraction of the CKM angle \\gamma. The method proposed by Atwood, Dunietz and Soni (ADS) makes use of a decay chain where color and Cabibbo suppression interfere, which produces large CP-violating asymmetries. The CDF experiment reports the first measurement at a hadron collider of branching fractions and CP-asymmetries of suppressed B^{-} \\rightarrow D^{0}h^{-} signals, where h is \\pi or K. Using 5.0 fb^{-1} of data we found a combined significance exceeding 5\\sigma and we determined the ADS parameters with accuracy comparable with B-factories.

2011-01-01

334

Design of the electromagnetic actuator for vibration control of a flexible rotor

Purpose: To obtain stabilized operation by preventing over heat in emergency cooling pumps upon accidents of flow regulators. Constitution: A pressure suppression chamber pool and a pressure vessel are communicated to each other with a pipeway and the water in the suppression pool is charged by a charging pump to the pipeway. The pipeway is interposed with an emergency cooling pump so as to feed water in the pipeway to the pressure vessel and a water source and the emergency cooling pumps are connected by way of a closed pipeway. Further, the closed pipeway and the pipeway interposed with the charging pump are communicated to each other by way of a connecting pipeway, to which are interposed an instrument for detecting the increase in the temperature of the emergency cooling pumps due to abnormality in the closed pipe (such as troubles in flow regulators) and outputting control signals and an electrically actuated valve controlled by a control ...

335

Bounding bubbles: the vertex representation of 3d Group Field Theory and the suppression of pseudo-manifolds

In order to suppress resonance vibrations of a rotor-shaft of rotating machinery, a rotor-shaft system elastically supported by ball bearings which are installed in electromagnetic actuators are proposed and the design method of some compensatory circuits in the controller to control the electromagnetic actuators in the system are explained. These actuators are controlled by the analog PID (proportional, integral, and derivative) controller. The compensation for nonlinearity of electromagnetic force, the negative stiffness of electromagnet, and the time lag of the coil current are discussed and the effective control circuits are proposed. The rotor-shaft system is replaced by the equivalent two-degree-of-freedom system and both impulse and frequency responses are numerically simulated for several parameters of PD-controller gain. By applying the principle of dynamic absorbers, optimum parameters are calculated. The gains of the control circuit are adjusted to give ...

1994-12-21

336

Boron enhanced diffusion due to high energy ion-implantation and its suppression by using RTA process

Based on recent work on simplicial diffeomorphisms in colored group field theories, we develop a representation of the colored Boulatov model, in which the GFT fields depend on variables associated to vertices of the associated simplicial complex, as opposed to edges. On top of simplifying the action of diffeomorphisms, the main advantage of this representation is that the GFT Feynman graphs have a different stranded structure, which allows a direct identification of subgraphs associated to bubbles, and their evaluation is simplified drastically. As a first important application of this formulation, we derive new scaling bounds for the regularized amplitudes, organized in terms of the genera of the bubbles, and show how the pseudo-manifolds configurations appearing in the perturbative expansion are suppressed as compared to manifolds. Moreover, these bounds are proved to be optimal.

2011-01-01

337

Boron enhanced diffusion due to high energy ion-implantation and its suppression by using RTA process

SIMS measurements revealed that high energy boron-implantation causes transient enhanced diffusion (TED) of a shallow dopant profile due to Si interstitials even for a relatively low dose of {approximately}2E13cm{sup {minus}2}. By systematic analysis, it is found that this anomalous diffusion is most significant in 700--800 C annealing, and it takes place in the initial stage (less than 30 sec for 800 C) of annealing. Moreover, this anomalous diffusion is more considerable than the enhanced diffusion during oxidation (OED) in practical device fabrication processes. It is found that rapid thermal annealing (RTA) at 1,000--1,100 C is effective for suppressing the transient enhanced diffusion and realizing a shallow channel profile for deep sub-micron devices.

1995-12-31

338

Antisense-mediated suppression of C-hordein biosynthesis in the barley grain results in correlated changes in the transcriptome, protein profile, and amino acid composition

SIMS measurements revealed that high energy boron-implantation causes transient enhanced diffusion (TED) of a shallow dopant profile due to Si interstitials even for a relatively low dose of #approx#2E13cm"-"2. By systematic analysis, it is found that this anomalous diffusion is most significant in 700--800 C annealing, and it takes place in the initial stage (less than 30 sec for 800 C) of annealing. Moreover, this anomalous diffusion is more considerable than the enhanced diffusion during oxidation (OED) in practical device fabrication processes. It is found that rapid thermal annealing (RTA) at 1,000--1,100 C is effective for suppressing the transient enhanced diffusion and realizing a shallow channel profile for deep sub-micron devices.

339

British Library Electronic Table of Contents (United Kingdom)

Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage protei...

2007-01-01

340

A new technique for surface modification in magnesium alloys by applying magnesium oxide coating

Subcellular distribution of ryanodine receptors in the cardiac muscle of carp (Cyprinus carpio).

A new technique was proposed in order to improve poor corrosion resistance in magnesium. That is, magnesium hydroxide film was formed on the surface of 3 N-Mg by artificial corrosion in 1% MgCl{sub 2} solution for 3.6 ks, which followed by oxidation at 673 K for 3.6 ks in air. Corrosion resistance was evaluated by salt immersion tests in 1% NaCl solution under in situ laser microscopic observations. Bubble evolution, which occurred immediately at the beginning of the immersion test in the case of non-treated specimen, was suppressed by the process. And also, formation of filiform corrosion was suppressed for about 3.6 ks, which occurred at 180 s in the non-treated specimen. (orig.)

2003-07-01

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341

Localization of sites of photoaffinity labeling of the large subunit of Escherichia coli ribosomes by arylazide derivative of puromycin

We examined the subcellular localization of ryanodine receptors (RyR) in the cardiac muscle of carp using biochemical, immunohistochemical, and electron microscopic methods and compared it with those of rats and guinea pigs. To achieve this goal, an anti-RyR antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced RyRs. Western blot analysis using this antibody detected a single RyR band following the SDS-PAGE of sarcoplasmic reticulum (SR) membranes from carp atrium and ventricle as well as from mammalian hearts and skeletal muscles. The carp heart band had slightly greater mobility than those of mammalian hearts. Although immunohistochemical staining showed evident striations corresponding to the Z lines in longitudinal sections of mammalian hearts, clusters of punctate staining, in contrast, were distributed ubiquitously throughout carp atrium and ventricle. Electron ...

2003-06-12

342

Localization of sites of photoaffinity labeling of the large subunit of Escherichia coli ribosomes by arylazide derivative of puromycin

Previous work showed that (TH)p-azidopuromycin photoaffinity labeled 70 S Escherichia coli ribosomes and that photoincorporation into 50 S subunit proteins was in the order L23 greater than L18/22 greater than L15. In the present work the authors report on immunoelectron microscopic studies of the complexes formed by p-azidopuromycin-modified 50 S subunits with antibodies to the N6,N6-dimethyladenosine moiety of the antibiotic. The p-azidopuromycin-modified 50 S subunits appear to be identical to unmodified control subunits in electron micrographs. Complexes of modified subunits with antibodies to the N6,N6-dimethyladenosine moiety of p-azidopuromycin were visualized in micrographs. Two regions of p-azidopuromycin photoincorporation were identified. The primary site, seen in about 75% of the complexes, is between the central protuberance and small projection, on the side away from the L7/L12 arm, in a region thought to contain the ...

1985-08-25

343

In vivo evaluation of a lead-labeled monoclonal antibody using the DOTA ligand

Previous work showed that ["3H]p-azidopuromycin photoaffinity labeled 70 S Escherichia coli ribosomes and that photoincorporation into 50 S subunit proteins was in the order L23 greater than L18/22 greater than L15. In the present work the authors report on immunoelectron microscopic studies of the complexes formed by p-azidopuromycin-modified 50 S subunits with antibodies to the N6,N6-dimethyladenosine moiety of the antibiotic. The p-azidopuromycin-modified 50 S subunits appear to be identical to unmodified control subunits in electron micrographs. Complexes of modified subunits with antibodies to the N6,N6-dimethyladenosine moiety of p-azidopuromycin were visualized in micrographs. Two regions of p-azidopuromycin photoincorporation were identified. The primary site, seen in about 75% of the complexes, is between the central protuberance and small projection, on the side away from the L7/L12 arm, in a region thought to contain the ...

344

Functional expression of anti-hepatitis B virus (HBV) preS2 antigen scFv by cspA promoter system in Escherichia coli and application as a recognition molecule for single-walled carbon nanotube (SWNT) field effect transistor (FET)

The aim of this study was to assess the utility of a radioimmunoconjugate containing a lead radionuclide for therapy and scintigraphy applications. The radioimmunoconjugate evaluated consisted of a bifunctional DOTA ligand and monoclonal antibody (MAb) B72.3 using athymic mice bearing LS-174T tumors, human colon carcinoma xenografts. In the studies reported here, the lead-203-DOTA complex itself was first demonstrated to have in vivo stability. MAb B72.3 was then conjugated with the DOTA ligand and labeled with "2"0"3Pb, and the immunoreactivity of B72.3 was maintained. The localization of the radioimmunoconjugate to tumor tissue and other select organs paralleled that of DOTA-"1"2"5I-B72.3, suggesting a similar metabolic pattern of the two radioimmunoconjugates. Thus, the DOTA-metal complex does not alter the behavior of the radioimmunoconjugate. Tumor localization of the "2"0"3Pb-DOTA-B72.3 conjugate was demonstrated with biodistribution studies as well as ...

1998-05-01

345

British Library Electronic Table of Contents (United Kingdom)

The preS2 antigens of hepatitis B virus (HBV), which causes a serious health problem in the world, have been implicated in hepatocyte cell binding and viral penetration. Therefore, the importance of antibody production against preS2 antigen for early diagnosis of HBV has been well established. In this study, the recombinant HBV preS2 single chain variable fragment (scFv) antibody was successfully expressed in E. coli with the novel cold shock vector (pCold) under the cspA promoter, and its expression level was compared with the pET vector under the T7 promoter. Additionally, a host with an oxidizing cytoplasm, E. coli trxB/gor double mutant, was used to improve the soluble expression. The anti-HBV preS2 scFv using pCold vector was successfully expressed in a soluble and functional form in ...

2010-01-01

346

Enzyme-linked immunosorbent assays for alpha-synuclein with species and multimeric state specificities.

Distribution of Ehrlichia canis among military workingn> dogs in the world and selected civilian dogs in the United States.

Abnormal intracellular deposition of aggregated ?-synuclein is the characteristic feature of a number of neurological disorders, including Parkinson's disease (PD). Although ?-synuclein is typically known as a cytosolic protein, a small amount is secreted by exocytosis in both monomeric and aggregated forms. The extracellular forms of ?-synuclein in human body fluids, such as cerebrospinal fluid (CSF) and blood plasma, might be a diagnostic target for PD and related diseases. Here, we characterized a new set of monoclonal antibodies against ?-synuclein, and using different combinations of antibodies, we established ELISA systems to specifically detect human ?-synuclein, mouse and human ?-synuclein together, and multimeric forms of ?-synuclein in biological samples. By employing the Tyramide signal amplification method, the sensitivity of the assay was significantly improved to detect a concentration as low as ?12.5 pg/ml. These assays might be ...

2011-05-31

347

An immunochemical approach to the study of DNA damage and repair. Technical progress report, May 1, 1989--April 30, 1992

Antibodies to Ehrlichia canis were detected by indirect immunofluorescence in sera from 233 of 2,077 (11%) military working dogs in various locations throughout the world and from 535 of 938 (57%) civilian dogs in the United States during a 1-year period of study. Overall, E canis infection rates ranged from 13% in the tropical and temperate zones below 45 degrees N to 8% in the cold zone north of 45 degrees N latitude. The highest antibody prevalence rate (24%) was found among a select population of dogs stationed between 40 degrees and 45 degrees north latitude (Japan and Okinawa). The seropositive military dogs did not have clinical signs of ehrlichiosis, thus indicating that the predominant form of infection was subclinical. On the other hand, 216 (23%) of the seropositive civilian dogs had various signs of the disease. The difference was attributed to the fact that the sera from civilian dogs were submitted by practitioners who suspected ...

1982-08-01

348

An immunochemical approach to the study of DNA damage and repair

The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits or to prepare monoclonal antibodies. We have been studying damages that are stable radiolysis products found in X-irradiated DNA and thus of potential biological consequence. Our aim is to build an in vitro and in vivo data base on the interactions between model DNA lesions and such cellular enzymes as DNA polymerases and repair endonucleases. Initial studies have focused on pyrimidine ring saturation products (thymine ...

1992-05-01

349

A CD33-specific single-chain immunotoxin mediates potent apoptosis of cultured human myeloid leukaemia cells.

The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits or to prepare monoclonal antibodies. We have been studying damages that are stable radiolysis products found in X-irradiated DNA and thus of potential biological consequence. Our aim is to build an in vitro and in vivo data base on the interactions between model DNA lesions and such cellular enzymes as DNA polymerases and repair endonucleases. Initial studies have focused on pyrimidine ring saturation products (thymine ...

1992-05-01

350

Radiolabelling of. cap alpha. -bungarotoxin to high specific radioactivity with complete retention of biological activity

A novel single-chain immunotoxin was constructed by combining a CD33-specific single chain Fv (scFv) antibody fragment with an engineered variant of Pseudomonas exotoxin A (ETA). The variant toxin carries the KDEL peptide at its C-terminus, a cellular peptide mediating improved retrograde transport to the endoplasmic reticulum. The purified recombinant fusion protein induced potent apoptosis of the human myeloid cell lines U937, HL-60 and THP-1. Up to 98% of U937 cells were eliminated after treatment for 72 h with a single dose of 500 ng/ml (c. 7 nmol/l). Killing was antigen-specific and occurred by apoptosis. A control protein, consisting of a CD19-specific scFv antibody fragment fused to the ETA-KDEL toxin, failed to induce death of the CD19-negative cell lines U937, HL-60 and THP-1. The CD33-ETA toxin also mediated apoptosis of fresh patient-derived acute myeloid leukaemia cells from bone marrow and peripheral blood. The pronounced ...

2006-04-01

351

Purification of cyclotron-produced {sup 203}Pb for labeling Herceptin

Radioiodinated ..cap alpha..-bungarotoxin, the venom of Bungarus multicinctus, is commonly used for the detection and quantification of nicotinic acetylcholine receptors (nAChR) and anti-nAChR antibodies. A radioiodination method is described which is simple and yields a fully active product which, after fractionation, has a specific activity tenfold higher (900 Ci/mmol) than those routinely used, resulting in enhanced sensitivity of assays and conservation of material, especially muscle extracts.

1984-10-01

352

Pretargeted radioimmunotherapy in tumored mice using an in vivo {sup 212}Pb/{sup 212}Bi generator

A simple and rapid procedure was developed for the purification of cyclotron-produced {sup 203}Pb via the {sup 203}Tl(d,2n) {sup 203}Pb reaction. A Pb(II) selective ion-exchange resin, with commercial name Pb Resin from Eichrom Technologies, Inc., was used to purify {sup 203}Pb from the cyclotron-irradiated Tl target with excellent recovery of the enriched Tl target material. The purified {sup 203}Pb was used to radiolabel the monoclonal antibody Herceptin. The in vitro and in vivo properties of the {sup 203}Pb radioimmunoconjugate were evaluated.

2005-04-01

353

Multicellular level dosimetry and low dose rate effects

Objective: Pretargeting is the concept that combines optimal delivery of the antibody and rapid capture and elimination of the radioactivity. In this study, we evaluated the potential of antibody pretargeting to enable the tumor-targeting {sup 212}Pb for in vivo generation of {sup 212}Bi for {alpha} particle radiotherapy. Methods: The {sup 212}Pb/{sup 212}Bi chelate of DOTA-biotin, as well as their {gamma}-emitting analogues, {sup 203}Pb and {sup 205}Bi, was prepared and characterized. The radiolabeled compounds were injected in animals for evaluation of tumor targeting and normal tissue uptake and retention. In the pretargeting protocol, injection of 400 {mu}g of NR-LU-10 antibody-streptavidin conjugate was given at t=0 h, then 100 {mu}g of N-acetyl-galatosamine-biotin clearing agent was injected at t=20-24 h; finally, 1 {mu}g of {sup 212}Pb/{sup 212}Bi-DOTA-biotin was injected 6 h later. Results: Both {sup 203}Pb and {sup ...

2005-10-01

354

Labeled cells as research, diagnostic and therapeutic tools

Building on the concepts of MIRD methods described by Webber, Watson and others at this Symposium on the Dosimetry of Administered Radionuclides, we will now consider how to apply these principles to several experimentally based open-quote macroclose quotes and multicellular models. Specifically, methods to calculate absorbed dose using different types of particulate emission (alpha, beta) with a variety of antibody carriers (IgG, F(ab')_2, Fab) for radioimmunotherapy (RIT) will be reviewed. Additionally, a discussion of direct measurement methods at the multicellular level will reveal the problems of tumor absorbed dose heterogeneity when applied to animal and clinical studies.

1989-09-21

355

Cutaneous leishmaniasis associated with jungle training.

Scientists at JPL have developed chemical and biological techniques using microspheres filled with drugs, electron-opaque metals, or radioactive, fluorescent, magnetic or electrically charged materials to label specific groups of cells. Synthetic polymeric microspheres are coupled with specific antibodies to form reagents called immunomicrospheres, which can seek out and attach themselves to any specific group of cells. These cell-labeling techniques, therefore, open new avenues not only to the basic study of cells but also to the diagnosis and treatment of many diseases, including cancer.

1983-09-01

356

Cutaneous gallium uptake in patients with AIDS with mycobacterium avium-intracellulare septicemia

In November 1977, 627 soldiers belonging primarily to the First Battalion, 82nd Airborne Division, stationed at Fort Bragg, were sent to the Canal Zone, Panama, for jungle warfare training. A medical surveillance program incorporating pre- and post-evaluations over a 6-month period with dermatologic examinations, questionnaires, and serologic tests was established. Ten cases of cutaneous leishmaniasis (1.6/100 men) were diagnosed by positive Leishmania culture. The demonstrated lack of sensitivity and specificity of the indirect fluorescent antibody test and the direct agglutination test render these serological methods useless as diagnostic screening methods in the early stages of this disease. PMID:7406101

1980-07-01

357

In vivo studies of the long-term "5"1Cr red cell survival of serologically incompatible red cell units

Gallium imaging is increasingly being used for the early detection of complications in patients with AIDS. A 26-year-old homosexual man who was HIV antibody positive underwent gallium imaging for investigation of possible Pneumocystis carinii pneumonia. Widespread cutaneous focal uptake was seen, which was subsequently shown to be due to mycobacterium avium-intracellulare (MAI) septicemia. This case demonstrates the importance of whole body imaging rather than imaging target areas only, the utility of gallium imaging in aiding the early detection of clinically unsuspected disease, and shows a new pattern of gallium uptake in disseminated MAI infection.

358

Autoradiographic analysis of radiolabeled anti-carcinoembryonic antigen monoclonal antibody CEA 102 in colorectal cancer using computed radiography

The long-term survival of serologically incompatible red cell units was measured in five patients with antibodies to high-frequency antigens. Initially, the survival of 1 ml of "5"1Cr-labeled incompatible red cells was measured over 1 hour. After demonstrating that the 1-hour survival times were successful (greater than 70%), each patient then received 5 ml of the same "5"1Cr-labeled red cells followed by the transfusion of the remainder of the red cell unit. The long-term T 1/2Cr survival for each case was patient 1 (anti-McCa), 15 days; patient 2 (anti-JMH), 12 days; patient 3 (anti-Kna), 31 days; patient 4 (anti-McCa), 12 days; and patient 5 (anti-Hya), 14 days. Each antibody tested in an in vitro homologous macrophage assay showed less than 5 percent phagocytosis. Anti-JMH was the only antibody to react with IgG subclass antisera and was determined to be IgG4. The macrophage assay, IgG subclass testing, and short-term ...

359

Autoradiographic analysis of radiolabeled anti-carcinoembryonic antigen monoclonal antibody CEA 102 in colorectal cancer using computed radiography

Anti-carcinoembryonic antigen monclonal antibody (MAb) CEA 102 was produced by immunization with purified CEA and the specific accumulation of radiolabeled CEA 102 in colorectal cancers was investigated by autoradiography of sugical specimens using Fuji Computed Radiography (FCR). Five patients with colorectal cancer were injected intravenously with {sup 131}I-labeled intact CEA 102 or its F(ab'){sub 2}. Primary tumor and liver metastases were successfully detected by external scanning with a gamma camera in 4 cases. Autoradiographic study of the surgical specimens using FCR showed predominant localization of {sup 131}I-labeled CEA 102 in primary tumors and liver metastases in all cases. Even a small liver metastasis (0.5 cm) was clearly visualized in the autoradiogram by FCR. The pixel distribution curves of the density of the respective tissues in the autoradiograms by FCR showed the heterogeneity of the distribution of administreted radiolabeled MAb in ...

1992-04-01

360

p16^INK4a Suppression by Glucose Restriction Contributes to Human Cellular Lifespan Extension through SIRT1-Mediated Epigenetic and Genetic Mechanisms

Anti-carcinoembryonic antigen monclonal antibody (MAb) CEA 102 was produced by immunization with purified CEA and the specific accumulation of radiolabeled CEA 102 in colorectal cancers was investigated by autoradiography of sugical specimens using Fuji Computed Radiography (FCR). Five patients with colorectal cancer were injected intravenously with "1"3"1I-labeled intact CEA 102 or its F(ab')_2. Primary tumor and liver metastases were successfully detected by external scanning with a gamma camera in 4 cases. Autoradiographic study of the surgical specimens using FCR showed predominant localization of "1"3"1I-labeled CEA 102 in primary tumors and liver metastases in all cases. Even a small liver metastasis (0.5 cm) was clearly visualized in the autoradiogram by FCR. The pixel distribution curves of the density of the respective tissues in the autoradiograms by FCR showed the heterogeneity of the distribution of administreted radiolabeled MAb in individual tumors, ...

1992-01-01

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361

beta. -Endorphin and related peptides suppress phorbol myristate acetate-induced respiratory burst in human polymorphonuclear leukocytes

Although caloric restriction (CR) has been shown to increase lifespan in various animal models, the mechanisms underlying this phenomenon have not yet been revealed. We developed an in vitro...Full Text Available

362

[Intraspecies antagonism of Sh. flexneri in an HEp-2 cell line model].

In the present study, the immunomodulatory effect of {beta}-endorphin ({beta}-E) and shorter pro-opiomelancortin (POMC) fragments was evaluated by assessing their influence on respiratory burst in human polymorphonuclear leukocytes (PMN). The effect of the peptides on phorbol myristate acetate (PMA)-stimulated production of reactive oxygen metabolites was measured in a lucigenin-enhanced chemiluminescence (CL) assay. Both POMC peptides with opiate-like activity and their non-opioid derivatives were tested. With the exception of {alpha}-E, PMA-stimulated respiratory burst was suppressed by all POMC fragments tested. A U-shaped dose-response relation was observed. Doses lower than 10{sup {minus}17}M and higher than 10{sup {minus}8}M were without effect. {beta}-E and dT{beta}E both suppressed PMA-induced oxidative burst in human PMN at physiological concentrations. {gamma}-E and dT{gamma}E proved to be less potent inhibitors, reaching maximal ...

1989-01-01

363

Viral suppression of multiple escape mutants by de novo CD8⁺T cell responses in a human immunodeficiency virus-1 Infected elite suppressor

The authors describe an effect of suppression of invasion of the guinea pig eye conjunctiva and the HEp-2 epithelial cells by virulent Sh. flexneri bacilli, with a simultaneous administration of the same dose of avirulent shigella mutants, genetically connected with them. The data of morphological study and experiments with 3H-glucose labeled shigellae carried out on the cell species model indicated that the bacterial competition for the specific sites for absorption on the epithelial cells underlay the observed phenomenon. PMID:331774

1977-06-01

364

The central tracking detectors for D/O/

Elite suppressors or controllers (ES) are HIV-1 infected patients who maintain undetectable viral loads without treatment. While HLA-B*57-positive ES are usually infected with virus that is unmutated...Full Text Available

365

The adiabatic engine:Global developments

Three types of drift chambers are being constructed for the Fermilab D/O/ experiment. The construction and readout of these chambers stress good spatial resolution, good two hit separation, and dE/dx. A 106 MHz FADC system with hardware zero suppression is being constructed to readout this system. 8 refs., 12 figs., 1 tab.

1988-01-01

366

Targeted suppression of Has2 mRNA in mouse cumulus cell-oocyte complexes by adenovirus-mediated short-hairpin RNA expression

This book presents papers on internal combustion engines. Topics considered include the influence of partial suppression of heat rejection on performance and emissions, duothermic combustion, turbochargers made of sintered silicon nitrides, heat flux, low heat rejection engines, exhaust energy recovery, combustion chamber insulation, computerized simulation, heat transfer, friction, hoop stress effects, and bonding ceramics and metals.

1986-01-01

367

Study of transient enhanced dopant diffusion in silicon and proposed limiting methods

RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This...Full Text Available

2009-06-01

368

Stabilization of synchrotron radiation beam at HASYLAB

The transient enhanced diffusion in crystalline silicon implanted with dopants ad followed by high temperature annealing to activate the dopants is introduced. The physical mechanisms of transient enhanced dopant diffusion are then reviewed together with a short introduction to the proposed suppressing methods. Finally, the perspectives with using high energy heavy ions in this field are briefly discussed

2001-09-01

369

Simvastatin suppresses LPS-induced MMP-1 expression in U937 mononuclear cells by inhibiting protein isoprenylation-mediated ERK activation

At DORIS II/HASYLAB a vertical beam position control system is in regular operation. It controls the position and angle of a photon beam simultaneously, and can stabilize beam movements up to frequencies of 0.1 Hz. To suppress beam vibrations up to 50 Hz and above, a prototype beam stabilization system for these frequencies was built and tested successfully. The present beam oscillations at the HASYLAB beamlines are explained, the quality of the beam position control system and the results of the prototype test are presented.

1989-07-01

370

SIV antigen immunization induces transient antigen-specific T cell responses and selectively activates viral replication in draining lymph nodes in retroviral suppressed rhesus macaques

Matrix metalloproteinase (MMP) plays a crucial role in periodontal disease and is up-regulated by oral Gram-negative, pathogen-derived LPS. In this study, we reported that simvastatin, a 3-hydroxyl-3-methylglutaryl-CoA...Full Text Available

2008-10-01

371

S179D prolactin diminishes the effects of UV light on epidermal gamma delta T cells

BackgroundHIV infection causes a qualitative and quantitative loss of CD4⁺T cell immunity. The institution of anti-retroviral therapy (ART) restores CD4⁺T...Full Text Available

372

Relativistic effects on chaos. Loss mechanism of runaway electrons in a tokamak

Epidermal gamma delta T cells (γδ T) and Langerhans cells (LC) are immune cells altered by exposure to ultraviolet radiation (UVB), a powerful stressor resulting in immune suppression....Full Text Available

2008-01-02

373

Quantum locking of mirrors in interferometric measurements

The relativistic motion of an electron is numerically analyzed in a tokamak having macroscopic magnetic turbulence. Stochasticity induced by the relativistic motion overwhelms the phase averaging effect, which provides a tokamak with an effective loss mechanism for the avoidance/suppression of runaway electron generation at a major disruption. On the other hand, electrons in the KAM (Kolmogorov-Arnold-Moser) region will be observed as a runaway snake. (author)

2001-12-01

374

Properties of A-15 Superconductors with Defects

We discuss the use of active control to reduce mirror position fluctuations at the quantum level. We have shown in a recent experiment that it is possible to reduce the thermal noise of a mirror by measuring and controlling its motion with an optomechanical sensor based on a high-finesse optical cavity. This approach can be extended to lock the mirror motion at the quantum level, and to suppress the quantum effects of radiation pressure in interferometric measurements such as gravitational-wave detectors. The sensitivity improvement is furthermore independent of losses in the interferometer.

2004-03-07

375

Preparation of Cluster States for Many Atoms in Cavity QED

It is suggested that the large reduction of the superconducting transition temperature Tc due to defects observed experimentally in some A-15 compounds is caused by smearing of a high peak in the density of states at the Fermi level. The influence of defects on other physical properties (the magnetic susceptibility ?, the elastic modulus Cs, the structural transformation temperature Tm and the electrical resistivity ?) is also discussed from the same point of view. We expect the anomalous temperature dependence of ?, Cs and ? will be suppressed by defects.

1978-05-01

376

Potent immune responses and in vitro pro-inflammatory cytokine suppression by a novel adenovirus vaccine vector based on rare human serotype 28

We propose a scheme for the generation of the cluster states for many atoms in cavity QED. In our scheme, the atoms are sent through nonresonant cavity fields in the vacuum states. The cavity fields are only virtually excited and no quantum information will be transferred from the atoms to the cavity fields. The advantage is that the cavities are suppressed during the procedure. The scheme can also be generalized to the ion trap system.

2007-07-15

377

Photodetachment of negative ion beams in the presence of a background gas

Adenovirus vaccine vectors derived from rare human serotypes have been shown to be less potent than serotype 5 (Ad5) at inducing immune responses to encoded antigens. To identify highly immunogenic...Full Text Available

2010-08-09

378

Instrument of millimetre wave radiation and its effect on malignant tumor in mice and its application in clinic

To suppress space charge blowup in an ion beam passing through a photoneutralizer, it is necessary to introduce some background gas. An analysis is presented of the neutralization of a high-energy, >200-keV negative deuterium ion beam, exposed to photodetachment while in the presence of deuterium. With a gas thickness of <0.01 Torr.cm, the neutral fraction in the output beam is found to be about the same as that gotten from the photoneutralizer operating in vacuum. Neutral atom beam injection for plasma heating is discussed.

1987-03-01

379

Inhibition of Melanoma Growth by Subcutaneous Administration of hTERTC27 Viral Cocktail in C57BL/6 Mice

An animated test is made for mice with malignant tumors irradiated by a self-made millimetre wave radiator for medical purpose. It is observed that S-180 sarcomas in mice after irradiation has been distinctly suppressed. And remarkable effects are shown through a lot of clinical practices on peptic ulcer, skin-deep ulcer, acute and chromic soft tissue injuries etc.

1995-12-31

380

Improvement of the parameters of shallow p"+-n-junctions in silicon by additional carbon implantation and step-by-step thermal treatments

BackgroundhTERTC27 is a 27 kDa C-terminal polypeptide of human telomerase reverse transcriptase that has previously been shown to reduce tumorigenicity of HeLa cells and suppress...Full Text Available

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381

Horizontal vibration suppression method suitable for super-high-speed elevators; Chokosoku elevator ni tekishita kago yokoshindo yokusei hoshiki

In this article carbon co-implantation and step-by-step thermal treatments of shallow p"+-n-junctions formation were used with the purpose of extended defect suppression and reduction of boron transient enhanced diffusion. A substantial improvement of the structural and electrical parameters of shallow p"+-n-junctions has been achieved by using the additional carbon implantation and step-by-step thermal treatments. (authors)

382

Homocysteine effects classical pathway of GPCR down regulation: G?_q/11, G?_12/13, G_i/o

Horizontal vibrations of elevator cars mainly occur because a car swings as roller guides installed at corners of a car frame move on a winding guide rail at high speeds. Rider comfort in high speed elevators is worsened by these vibrations. Conventional active dampers suppressing horizontal vibrations using ac servo motors make cars heavier so driving power becomes larger, and they are not easily applied to existing elevators. An active damping control method suited to super-high-speed elevators is which can solve these problems. The method suppresses vibrations by generating only enough magnetic force needed to suppress them only when vibrations of the car franc are produced. The vibrations are detected using acceleration detectors and magnets installed on left and right sides of the car frame. A computer simulator was made to analyze phenomena of car vibrations and to verify effects of the proposed magnetic damping ...

1998-03-01

383

High energy proton-proton scattering and multiple scattering model including inelastic intermediate states

G protein-coupled receptors (GPCRs) are known to modulate intracellular effectors involved in cardiac function. We recently reported homocysteine (Hcy)-induced ERK-phosphorylation was suppressed...Full Text Available

2009-01-01

384

Curcumin eliminates oxidized LDL roles in activating hepatic stellate cells by suppressing gene expression of lectin-like oxidized LDL receptor-1

The cross sections for proton-proton elastic scattering at Fermilab and ISR energies are investigated in the framework of the multiple constituent scattering formalism, including the contributions of the inelastic intermediate states. We find that the higher-order multiple scattering amplitudes are strongly suppressed due to the presence of the inelastic intermediate states. The calculated cross sections reproduce the high energy experimental data fairly well. (author).

385

Assessment of Glucagon-Like Peptide-1 Analogue and Renin Inhibitor on the Binding and Regulation of GLP-1 Receptor in Type 1 Diabetic Rat Hearts

Type II diabetes mellitus (T2DM) is often accompanied by non-alcoholic steatohepatitis (NASH) and associated with hypercholesterolemia, i.e. increased levels of plasma low-density...Full Text Available

2009-11-01

386

Apoptotic pathways as regulators of recombination

This study focuses on the effects of long-term renin-angiotensin system suppression and/or incretin mimetic therapies on the regulation and binding affinity of GLP-1 to its receptor in the coronary...Full Text Available

2011-01-01

387

An imaging. gamma. -ray detector with scatter rejection for beam position control in radiotherapy

Apoptosis, or programmed cell death (PCD), is a fundamental process that protects organismal integrity. In earlier work, we demonstrated that over-expression of either of two anti-apoptotic members of the BCL-2 family (BCL-2 or BCL-X L could elevate the frequency of radiation-induced mutations at the autosomal TK1 locus in human TK6 lymphoblasts that express wild-type TP53. Ectopic expression of BCL-X L also elevated the frequencies of double-strand break-induced gene conversion. The purpose of this study is to determine if BCL-2 family proteins promote radiation mutagenesis indirectly through their suppression of PCD, or whether the 'pro-mutagenic' function of these proteins can be separated from their anti-apoptotic function. We developed stable transfectants of TK6 cells that express a mutated form of BCL-X L with a single amino acid substitution in the BH1 domain that is known to interfere with the ability to suppress PCD (BCL-X L ...

2003-08-17

388

Active RF filter for high voltage transmission lines

An imaging detector for {gamma}-rays ({approx equal}1 MeV) based on minicell MWPCs with converters and a position resolution of {Delta}x=0.5 mm has been developed and tested. Very high rate capability (>10{sup 8}/cm{sup 2} s) and a special readout for suppression of scattered radiation are implemented allowing to enhance the measured contrast. The relevant physical processes are discussed and first pictures are presented. (orig.).

1991-12-01

389

Local Quark-Hadron Duality and Magnetic Form Factors of Bound Proton

A new technique is described in this paper for a general active radio frequency (RF) filter trap that can be used for suppressing noise or interference on high voltage (HV) transmission lines. The technique exploits the Miller effect of an RF amplifier in conjunction with a special sensing circuit, and is potentially far more economical to implement than conventional techniques that use passive HV filter components.

1993-07-01

390

Tumor necrosis factor-? serum levels in healthy smokers and nonsmokers

We discuss the consequence of local duality for elastic scattering, and derive a model-independent equation between structure functions at x ? 1 and elastic electromagnetic form factors. Then the electromagnetic form factors of proton are discussed using the quark-hadron duality theory. We also debate the form factor of proton in a bound state. It may be an effective approach to study the form factor of proton in media.

2005-08-01

391

Translation Elongation Factor 1A Facilitates the Assembly of the Tombusvirus Replicase and Stimulates Minus-Strand Synthesis

Background:Tobacco smoking is the most important risk factor for chronic obstructive pulmonary disease (COPD) development. Inhaled cigarette smoke can induce tumor necrosis factor-α...Full Text Available

2010-01-01

392

On-line real-time measurements of decontamination factor for a low-level waste incinerator

Replication of plus-strand RNA viruses depends on host factors that are recruited into viral replicase complexes. Previous studies showed that eukaryotic translation elongation factor (eEF1A) is one...Full Text Available

2010-11-01

393

On-line real-time measurements of decontamination factor for a low-level waste incinerator

The experiments at the incinerator for low-level wastes processing, (containing transuranium radionuclides) are described. Air decontamination factors and detector readings correction factors for efficiency and absorption are indicated.

1983-01-01

394

Immunoradiometric measurement of the factor VIII procoagulant antigen.

The experiments at the incinerator for low-level wastes processing, (containing transuranium radionuclides) are described. Air decontamination factors and detector readings correction factors for efficiency and absorption are indicated.

395

Elk-1 a Transcription Factor with Multiple Facets in the Brain

A fluid-phase immunoradiometric assay has been developed which identifies an antigen on the Factor VIII (antihemophilic factor) procoagulant protein. This sensitive and quantitative assay is not influenced...Full Text Available

1978-11-01

396

Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood

The ternary complex factor (TCF) Elk-1 is a transcription factor that regulates immediate early gene (IEG) expression via the serum response element (SRE) DNA consensus site. Elk-1 is associated with...Full Text Available

397

Demographic, Dietary, and Urinary Factors and 24-h Urinary Calcium Excretion

Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization,...Full Text Available

2011-01-01

398

Biochemical characterization of the molecular interaction between recombinant basic fibroblast growth factor and a recombinant soluble fibroblast growth factor receptor.

Background and objectives: Higher urinary calcium is a risk factor for nephrolithiasis. This study delineated associations between demographic, dietary, and urinary factors and 24-h urinary calcium.Design,...Full Text Available

2009-12-01

399

A biological standard for measurement of blood coagulation factor VIII activity

The extracellular domain of human fibroblast growth factor receptor (XC-FGF-R) was expressed in Escherichia coli. The protein was purified to homogeneity and the interaction with basic fibroblast growth...Full Text Available

1993-09-15

400

Radium concentration factors and their use in health and environmental risk assessment

Because of the instability of Factor VIII (antihaemophilic factor) in plasma in vitro, and since evidence has accumulated that the level of activity varies significantly between samples...Full Text Available

1971-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Radium concentration factors and their use in health and environmental risk assessment

Radium is known to be taken up by aquatic animals, and tends to accumulate in bone, shell and exoskeleton. The most common approach to estimating the uptake of a radionuclide by aquatic animals for use in health and environmental risk assessments is the concentration factor method. The concentration factor method relates the concentration of a contaminant in an organism to the concentration in the surrounding water. Site specific data are not usually available, and generic, default values are often used in risk assessment studies. This paper describes the concentration factor method, summarizes some of the variables which may influence the concentration factor for radium, reviews reported concentration factors measured in marine environments and presents concentration factors derived from data collected in a study in coastal Louisiana. The use of generic default ...

1991-01-01

402

Pacification: The Overall Strategy in South Vietnam

Radium is known to be taken up by aquatic animals, and tends to accumulate in bone, shell and exoskeleton. The most common approach to estimating the uptake of a radionuclide by aquatic animals for use in health and environmental risk assessments is the concentration factor method. The concentration factor method relates the concentration of a contaminant in an organism to the concentration in the surrounding water. Site specific data are not usually available, and generic, default values are often used in risk assessment studies. This paper describes the concentration factor method, summarizes some of the variables which may influence the concentration factor for radium, reviews reported concentration factors measured in marine environments and presents concentration factors derived from data collected in a study in coastal Louisiana. The use of generic default ...

1991-12-31

403

Occupational Asthma: Etiologies and Risk Factors

... implementation." 4 1 Factors Affecting Rural Construction. Two new factors profoundly modify today's pacification equa- tion. ...

1966-04-22

404

Nucleon Form Factor Measurements and Interpretation

The purpose of this article is to critically review the available evidence pertaining to occupational, environmental, and individual factors that can affect the development of occupational asthma (OA)....Full Text Available

2011-07-01

405

Immune aspects of sarcoidosis.

The data base for the form factors of the nucleon obtained from elastic ep scattering is discussed, as well as some recent developments in their calculation.

2007-08-22

406

Although the initiating factor(s) is unknown, it is now accepted that pulmonary sarcoidosis develops as a result of an over-stimulated local cellular immune response. Starting as a lymphocytic alveolitis,...Full Text Available

1988-07-01

407

Human factors

Factoring Potential Accelerator Transmutation of Waste Demonstrations into Accelerator Production of Tritium Design Planning

This is a presentation on Human Factors in reactor operations. It discusses issues that deal with power plant operations, training and design, operational effectiveness and safety, supporting people to achieve effective and error free performance.

2002-07-01

408

Deterministic Factors of Stock Networks based on Cross-correlation in Financial Market

Factoring Potential Accelerator Transmutation of Waste Demonstrations into Accelerator Production of Tritium Design Planning

1998-01-01

409

Clinical significance of unilateral sinusitis.

The stock market has been known to form homogeneous stock groups with a higher correlation among different stocks according to common economic factors that influence individual stocks. We investigate the role of common economic factors in the market in the formation of stock networks, using the arbitrage pricing model reflecting essential properties of common economic factors. We find that the degree of consistency between real and model stock networks increases as additional common economic factors are incorporated into our model. Furthermore, we find that individual stocks with a large number of links to other stocks in a network are more highly correlated with common economic factors than those with a small number of links. This suggests that common economic factors in the stock market can be understood in terms of deterministic factors.

2007-01-01

410

Multi-modality treatment of primary nonresectable intrahepatic cholangiocarcinoma with "1"3"1I anti-CEA--a Radiation Therapy Oncology Group Study

In general, the etiologic factors of chronic paranasal sinusitis are systemic conditions such as nutrition, predisposition, allergy, and local factors such as nasal anatomic conditions. Among these...Full Text Available

1986-09-01

411

Epitope mapping and functional analysis of sigma A and sigma NS proteins of avian reovirus

Thirty-seven patients with primary nonresectable intrahepatic cholangiocarcinoma (57% with prior treatment and/or metastasis) were prospectively treated with external radiation, chemotherapy, and "1"3"1I labelled anti-CEA. Therapy began in all trials with whole liver irradiation (21.0 Gy, 3.0 Gy/Fx, 4 days/week, 10 MV photons) with alternate treatment day chemotherapy (Adriamycin, 15 mg + 5-FU, 500 mg). One month after external beam therapy, chemotherapy was given (Adriamycin, 15 mg + 5-FU, 500 mg) followed the next day by the first administration of "1"3"1I anti-CEA. The treatment schedule used was 20 mCi day 0; 10 mCi day 5 as an outpatient. This schedule was derived from tumor dose estimates which indicated that 20 mCi (8-10 mCi/mg IgG) was sufficient to achieve tumor saturation with a tumor effective half-life of 3 to 5 days, depending upon the species of animal from which the antibody was obtained. The median tumor dose for the 20 mCi + 10 mCi regimen was 6.2 ...

412

The health impact of pemphigus vulgaris and pemphigus foliaceus assessed using the Medical Outcomes Study 36-item short form health survey questionnaire

We have previously shown that avian reovirus (ARV) #sigma#A and #sigma#NS proteins possess dsRNA and ssRNA binding activity and suggested that there are two epitopes on #sigma#A (I and II) and three epitopes (A, B, and C) on #sigma#NS. To further define the location of epitopes on #sigma#A and #sigma#NS proteins and to further elucidate the biological functions of these epitopes by using monoclonal antibodies (MAbs) 62, 1F9, H1E1, and 4A123 against the ARV S1133 strain, the full-length and deletion fragments of S2 and S4 genes of ARV generated by polymerase chain reaction (PCR) were cloned into pET32 expression vectors and the fusion proteins were overexpressed in Escherichia coli BL21 strain. Epitope mapping using MAbs and E. coli-expressed deletion fragments of #sigma#A and #sigma#NS of the ARV S1133 strain, synthetic peptides, and the cross reactivity of MAbs to heterologous ARV strains demonstrated that epitope II on #sigma#A was located at amino acid residues ...

2005-02-20

413

British Library Electronic Table of Contents (United Kingdom)

Summary Background Pemphigus vulgaris and pemphigus foliaceus are rare, potentially life-threatening, autoimmune disorders characterized by antibodies to epidermal adhesion molecules. Clinical characteristics are painful chronic erosions of mucous membranes and of the skin. There are only few published studies on the impact of the disease on the health status (HS) of patients with these conditions. Objectives To assess the impact of disease on the HS of patients with pemphigus. Methods Fifty-eight patients enrolled at the Bullous Skin Diseases Unit of IDI-IRCCS in the period January-June 2006 were assessed for their HS using the Medical Outcomes Study 36-item short form health survey (SF-36) questionnaire and for anxiety and depression using the Institute for Personality and Ability Testin...

2008-01-01

414

Serological testing-An alternative to boosters?

British Library Electronic Table of Contents (United Kingdom)

The issue of the duration of immunity, particularly for the modified live viral components of veterinary vaccines, has been a significant part of the recent vaccination debate. One manufacturer has increased the recommended booster interval for these components to 3 years give name and another now states `up to 4 years' immunity.There remain many unanswered questions regarding this duration of immunity (DOI). Studies suitable for data sheet claims are time consuming and costly and can only be performed in laboratory dogs under tightly controlled conditions. Evidence from rabies serology testing in the UK shows that the response of individual animals to routine vaccination is highly variable. Much of the published field evidence on the persistence of antibody titres originates from North Am...

2006-01-01

415

Radioimmunoassay data processing, ch. 2

Purification of cyclotron-produced "2"0"3Pb for labeling Herceptin

The problems of radioimmunoassay data processing with a fairly big computer are investigated in detail. The logit transformation, introduced by Rodbard et al. in 1968, and the related mathematical operations are presented. From logit-ln plots the confidence limits for the calculation of the antigen concentration can be predicted as well as the minimum detectable dose (i.e. the sensitivity of the assay). The Scatchard plot is used to calculate the equilibrium constant of the antigen-antibody coupling reaction and the binding capacity. A 4-parameter logistic curve fitting method is developed and combined with a method to carry out a multi-component Scatchard analysis. Both a weighted and an unweighted linear regression procedure are evaluated for use in calculating the plasma renin activity from the angiotensin I amounts determined by radioimmunoassay. Improvements for Rodbard's computer programmes are proposed.

1978-02-15

416

No detectable XMRV in subjects with chronic fatigue syndrome from Quebec

A simple and rapid procedure was developed for the purification of cyclotron-produced "2"0"3Pb via the "2"0"3Tl(d,2n) "2"0"3Pb reaction. A Pb(II) selective ion-exchange resin, with commercial name Pb Resin from Eichrom Technologies, Inc., was used to purify "2"0"3Pb from the cyclotron-irradiated Tl target with excellent recovery of the enriched Tl target material. The purified "2"0"3Pb was used to radiolabel the monoclonal antibody Herceptin. The in vitro and in vivo properties of the "2"0"3Pb radioimmunoconjugate were evaluated.

2005-04-01

417

British Library Electronic Table of Contents (United Kingdom)

We investigated the presence of XMRV in a cohort of Quebec patients with chronic fatigue syndrome (CFS). DNA was purified from activated peripheral blood mononuclear cells (PBMCs) and PCR was used to detect XMRV gag and env in 72 patients. Anti-XMRV antibodies were searched in sera of 62 patients by Western blot analysis. Attempts to detect XMRV antigens was made, using immunofluorescence with Gag anti-p30 antiserum on activated PBMC from 50 patients. Plasma viremia was measured by RT-PCR on 9 subjects. Finally, detection of infectious virus in 113 CFS subjects was made by co-culture of PHA+IL-2 activated PBMC with human LNCaP carcinoma cells, and by infecting the same susceptible cells with plasma, using a reverse transcriptase (RT) assay as a readout in both experiments. No detection of ...

2011-01-01

418

Mutation analysis of KRAS prior to targeted therapy in colorectal cancer: development and evaluation of quality by a European external quality assessment scheme

British Library Electronic Table of Contents (United Kingdom)

In Europe, the use of anti-EGFR monoclonal antibodies is restricted to Kirsten RAS (KRAS) wild-type colorectal tumors. Information on the KRAS status of the patients tumor is thus key for clinical practice; however, there is little guidance or definition on which KRAS mutations to assess and how to assess them. To ensure the consistency and the quality of KRAS test results in Europe, an interlaboratory control network needs to be set up. This pilot study aimed to identify the variables that need to be assessed in a quality control scheme and to provide a first assessment in a selected set of laboratories. Fourteen different tumor cases were circulated between 13 laboratories by a central laboratory acting as the referent for the mutation status determination. This study illustrated that of...

2011-01-01

419

Leishmania major ascorbate peroxidase overexpression protects cells against reactive oxygen species-mediated cardiolipin oxidation

British Library Electronic Table of Contents (United Kingdom)

Heme peroxidases are a class of multifunctional redox-active proteins found in all organisms. We recently cloned, expressed, and characterized an ascorbate peroxidase from Leishmania major (LmAPX) that was capable of detoxifying hydrogen peroxide. Localization studies using green fluorescent protein fusions revealed that LmAPX was localized within the mitochondria by its N-terminal signal sequence. Subcellular fractionation analysis of the cell homogenate by the Percoll density-gradient method and subsequent Western blot analysis with anti-LmAPX antibody further confirmed the mitochondrial localization of mature LmAPX. Submitochondrial fractionation analysis showed that the mature enzyme (?3.6?kDa shorter than the theoretical value of the whole gene) was present in the intermembrane space ...

2008-01-01

420

Involvement of Rh blood group polypeptides in the maintenance of aminophospholipid asymmetry

Insight into Thyroid-Stimulating Autoantibody Interaction with the Thyrotropin Receptor N-Terminus Based on Mutagenesis and Re-Evaluation of Ambiguity in This Region of the Receptor Crystal Structure

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, "1"2"5I-azido-PS, and with an inhibitor of PS transport, "1"2"5I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

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421

British Library Electronic Table of Contents (United Kingdom)

Background: Thyroid-stimulating autoantibodies (TSAb) bind to the thyrotropin receptor (TSHR) extracellular domain, or ectodomain (ECD), comprising a leucine-rich repeat domain (LRD) linked by a hinge region to the transmembrane domain (TMD). The LRD (residues 22???260; signal peptide 1???21) contains two disulfide-bonded loops at its N-terminus. In the crystal structure of the isolated LRD complexed with human TSAb monoclonal antibody (mAb) M22, N-terminal disulfide loop 1 (residues 22???30) could not be determined because of crystal disorder. Nevertheless, present crystal structure data are interpreted to exclude a role for the LRD N-terminal disulfide loops in the TSAb epitope(s), contradicting prior functional evidence of a role for these loops in TSAb function. Materials and Methods: ...

2011-01-01

422

Immunofluorescence analysis of the internal brain anatomy of Nereis diversicolor (Polychaeta, Annelida)

British Library Electronic Table of Contents (United Kingdom)

Comparative analyses of neuroanatomical characters can make valuable contributions to the inference of phylogenetic relationships. Whereas investigations in this field are numerous for arthropods, in-depth studies on other protostomes are sparse. Here, we provide a survey of the internal neuroarchitecture of the brain of the aciculate ragworm Nereis diversicolor (Polychaeta, Annelida). Descriptions are based on confocal laser scanning microscope analyses of brain sections labeled with the nuclear marker DAPI and antibodies raised against FMRF-amide, serotonin, and histamine. Autofluorescence of the nervous tissue has been utilized to further elucidate the anatomical structures of the brain. The architecture of two major brain compartments, i.e., the paired mushroom bodies and the central o...

2008-01-01

423

Immune responses induced by spirochetal outer membrane lipoproteins and glycolipids

British Library Electronic Table of Contents (United Kingdom)

The class of Spirochetes comprises a wide array of clinically important pathogens, including Treponema pallidum causing syphilis as well as Borrelia burgdorferi, the agent of Lyme disease (LD). Diseases caused by spirochetes are characterized by specific sequelae of host reactions, and also by characteristic antibody response patterns. Over the last decades, research on the interaction of spirochetes with the hosts immune system had a strong emphasis on outer membrane lipoproteins. In fact, these structures have been convincingly shown to activate immune cells via CD14 and Toll-like receptor (TLR)-2, and recent data also indicate an interaction with lipopolysaccharide (LPS)-binding protein (LBP). In particular, the interaction of B. burgdorferi with TLR-2 could not only be demonstrated in ...

2008-01-01

424

Homologous radioimmunoassay for human prolactin

Heparin binding sites on Ross River virus revealed by electron cryo-microscopy

Although thee are descriptions of a range of radioimmunoassays for human prolactin in various biological fluids, only one of these is an homologous assay using human prolactin as the reference standard and tracer as well and an anti-human prolactin antiserum (Sinha, Y.N., Selby, F.W.; Lewis, U.; and Vanderlaan, W.P., 1973, J. Clin. Endocr., Vol. 36, 509). A homologous radioimmunoassay using human putuitary prolactin has been developed. The separation method is based on the double antibody solid phase system. Cross reactivity with human growth hormone (GH), placental lactogen (HPL), the pituitary protein hormones and prolactins of various species were studied as were values found in normal subjects in basal conditions and after a TRH injection. (author).

425

Estimates of quantitative genetic parameters of immunological traits in the chicken.

Cell surface glycosaminoglycans play important roles in cell adhesion and viral entry. Laboratory strains of two alphaviruses, Sindbis and Semliki Forest virus, have been shown to utilize heparan sulfate as an attachment receptor, whereas Ross River virus (RRV) does not significantly interact with it. However, a single amino acid substitution at residue 218 in the RRV E2 glycoprotein adapts the virus to heparan sulfate binding and expands the host range of the virus into chicken embryo fibroblasts. Structures of the RRV mutant, E2 N218R, and its complex with heparin were determined through the use of electron cryo-microscopy and image reconstruction methods. Heparin was found to bind at the distal end of the RRV spikes, in a region of the E2 glycoprotein that has been previously implicated in cell-receptor recognition and antibody binding.

2005-02-20

426

Cytoplasmic p63 immunohistochemistry is a useful marker for muscle differentiation: an immunohistochemical and immunoelectron microscopic study

Three in vivo assays were used to measure the immunocompetence of chickens in two generations of a selection experiment. The obtained data were used to estimate the variance components for sire and dam for antibody production to Pasteurella multocida and Mycoplasma gallisepticum vaccines, for T-cell-mediated immunity evaluated by a phytohemagglutinin wing web assay, and for clearance of foreign particles from the circulatory system. Heritabilities of and genetic correlations among these immunological traits were calculated from the sire variance components. Heritability estimates of the immunological traits based on the sire component of variance ranged from .06 to .53, and genetic correlations among immunological traits were generally negative. PMID:1956847

1991-10-01

427

British Library Electronic Table of Contents (United Kingdom)

TP63, a member of the TP53 gene family, is a nuclear marker of myoepithelial cells. Antibody against p63 is frequently used to aid in the diagnosis of prostate carcinoma, as well as in the identification of myoepithelial cells in other tissues including the breast. p63 is also a marker for squamous cell carcinoma. Recently, it was found that all p53 family members are involved in regulating the process of muscle differentiation through the retinoblastoma (RB) protein. Ablation of these p53 family functions blocks the differentiation program and promotes malignant transformation by enabling cooperating oncogenes to transform myoblasts. We therefore studied p63 expression in a number of neoplasms with myogenic differentiation. Immunohistochemical staining for p63 was performed on paraffin se...

2011-01-01

428

Copper-64 Radiopharmaceuticals for PET Imaging of Cancer: Advances in Preclinical and Clinical Research

British Library Electronic Table of Contents (United Kingdom)

Summation Copper-64 (T1/2?=?12.7 hours; ?+, 0.653?MeV [17.8 %]; ??, 0.579?MeV [38.4 %]) has decay characteristics that allow for positron emission tomography (PET) imaging and targeted radiotherapy of cancer. The well-established coordination chemistry of copper allows for its reaction with a wide variety of chelator systems that can potentially be linked to peptides and other biologically relevant small molecules, antibodies, proteins, and nanoparticles. The 12.7-hours half-life of 64Cu provides the flexibility to image both smaller molecules and larger, slower clearing proteins and nanoparticles. In a practical sense, the radionuclide or the 64Cu-radiopharmaceuticals can be easily shipped for PET imaging studies at sites remote to the production facility. Due to the versatility of 64Cu, ...

2009-01-01

429

Comparative biochemistry of betaine biosynthesis and accumulation in diverse dicot families

Biomagnetic separation of Salmonella Typhimurium with high affine and specific ligand peptides isolated by phage display technique

Salt stress elicits betaine accumulation to high levels in species from several diverse dicot families (Chenopodiaceae, Amaranthaceae, Convolvulaceae, Solanaceae, and Asteraceae). FAM-MS studies with deuterated precursors showed that species from all these families synthesize betaine from choline. Enzyme assays and immunotitration data showed that all accumulating species contained betaine aldehyde dehydrogenase (BADH) enzyme activity recognized by antibodies raised against purified BADH isolated from Spinacia oleracea. Immunoblotting indicated that the BADH monomer was in all cases of Mr {approx} 63,000. The similarity of BADH in the different species is consistent with a single evolutionary origin for the betaine pathway. This was supported by the presence in immunoblots of a cross-reacting band at Mr {approx} 63,000 in Magnolia x Soulangiana, a primitive angiosperm.

1989-04-01

430

A new approach to the isolation and characterization of wheat flour allergens

Analyses of food-borne pathogens are of great importance in order to minimize the health risk for customers. Thus, very sensitive and rapid detection methods are required. Current conventional culture techniques are very time consuming. Modern immunoassays and biochemical analysis also require pre-enrichment steps resulting in a turnaround time of at least 24 h. Biomagnetic separation (BMS) is a promising more rapid method. In this study we describe the isolation of high affine and specific peptides from a phage-peptide library, which combined with BMS allows the detection of Salmonella spp. with a similar sensitivity as that of immunomagnetic separation using antibodies.

2007-04-15

431

British Library Electronic Table of Contents (United Kingdom)

Summary Background The incidence of food allergy to wheat is increasing. Its diagnosis depends on the purity of major allergens and their inclusion in tests. Isolation and characterization of wheat allergens are therefore of utmost importance. Objective To purify and identify wheat flour allergens most frequently recognized by patients' IgE antibodies and to study their allergenicity. Methods Water/salt-soluble extracts from wheat flour were prepared and separated using a combination of ultrafiltration, isoelectric focusing and liquid chromatography. Purified proteins were analysed by immunoblotting using pooled sera from patients with atopic dermatitis who possessed IgE specific to wheat. Wheat proteins found to bind IgE were subsequently identified by matrix-assisted laser desorption/ion...

2011-01-01

432

A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies

British Library Electronic Table of Contents (United Kingdom)

Abstract One of the most significant problems in industrial bioprocessing of recombinant proteins using engineered mammalian cells is the phenomenon of cell line instability, where a production cell line suffers a loss of specific productivity (qP). This phenomenon occurs with unpredictable kinetics and has been widely observed in Chinese hamster ovary (CHO) cell lines and with all commonly used gene expression systems. The underlying causes (both genetic and physiological) and the precise molecular mechanisms underpinning cell line instability have yet to be fully elucidated, although recombinant gene silencing and loss of recombinant gene copies have been shown to cause qP loss. In this work we have investigated the molecular mechanisms underpinning qP instability over long-term sub-cult...

2011-01-01

433

Area Factor Determinations for an Industrial Worker Exposed to a Concrete Slab End-State

Upgrading of brown coal by slurry-dewatering; Kattan no yuchu dassui ni yoru clean kotai nenryo no seizo

The U.S. Department of Energy's (DOE) Savannah River Site (SRS) is decommissioning many of its excess facilities through removal of the facility structures leaving only the concrete-slab foundations in place. Site-specific, risk-based derived concentration guideline levels (DCGLs) for radionuclides have been determined for a future industrial worker potentially exposed to residual contamination on these concrete slabs as described in Jannik. These risk-based DCGLs were estimated for an exposure area of 100 m2. During deactivation and decommissioning (D and D) operations at SRS, the need for area factors for larger and smaller contaminated areas arose. This paper compares the area factors determined for an industrial worker exposed to a concrete slab end-state for several radionuclides of concern at SRS with 1) the illustrative area factors provided in MARSSIM, 2) the area correction factors provided in ...

2008-01-01

434

The influence of gas on the structure of disk merger remnants

This paper describes an outline of solid fuel production process from brown coal and the investigation results of its elemental techniques. Dried coal is produced by this process which consists of a dewatering of crushed brown coal in oil-based solvent, a solid and liquid separation of slurry, and a remained oil recovery by heating. This process is characterized by the higher thermal efficiency compared with usual drying and the restraint of spontaneous combustion of product coal. It was revealed that solid fuel with low moisture, low ash, low sulfur, and suppressed spontaneous combustion property can be produced from Australian brown coal through this process. From the comparison between kerosene and fuel oil A, it was confirmed that the oil content during dewatering was smaller and the oil recovery by heating was easier by using a solvent with lower boiling point. It was also confirmed that the spontaneous combustion property can be ...

1996-10-28

435

Suppression of pitting corrosion with passive film modification on type 304 stainless steel by ultra-violet light irradiation; Shigaikoshosha ni yoru Type 304 stainless ko no fudotai himaku kaishitsu to koshoku yokusei

We present a large set of merger simulations of early-type disc galaxies with mass ratios of 1:1 and 3:1 and 10% of the total disc mass in gas. In contrast to the collisionless case equal-mass mergers with gas do not result in very boxy remnants which is caused by the suppression of box orbits and the change of the projected shape of minor-axis tube orbits in the more axisymmetric remnants. The isophotal shape of 3:1 remnants and the global kinematic properties of 1:1 and 3:1 remnants are only weakly affected by the presence of gas. 1:1 remnants are slowly rotating whereas 3:1 remnants are fast rotating and discy. The shape of the stellar LOSVD is strongly influenced by gas. The LOSVDs of collisionless remnants have broad leading wings while their gaseous counterparts show steep leading wings, more consistent with observations of elliptical galaxies. We show that this change is also caused by the suppressed populating of box orbits and it is ...

2006-01-01

436

Support effects on CO hydrogenation over Ru/zeolite catalysts

In this study, the effects of 325nm wavelength ultraviolet light irradiation on pitting corrosion behavior of type 340 stainless steel in a neutral chloride solution are studied. Further, the change of passive film with the light irradiation is analyzed using x-ray photoelectron spectroscopy. The mains results obtained therefrom are stated below. Pitting potential can be shifted in noble direction by the ultraviolet light irradiation. The effect of ultraviolet light irradiation is ore prominent in the pitting corrosion process than that in the passive film formation. The result of the analysis in terms of the birth and death stochastic probability process shows that pitting corrosion rate is decreased remarkably by the ultraviolet light irradiation at the formation of passive film, while the repassivation is slightly expedited by the ultraviolet light irradiation. On the other hand, the repassivation rate is increased a little by the ultraviolet light irradiation during the pitting ...

1998-06-20

437

Summary report, California Department of Forestry and Fire Protection evaluation of full-face air-purifying respirators for wildland fire fighting use

Hydrogenation of carbon monoxide at 101.3 kPa has been studied over a series of ion-exchanged Ru catalysts supported on NaX, NaY, KL, Na mordenite, and HY zeolites. The type of zeolite had pronounced effects on the activity and selectivity of the Ru. The specific activity would appear to be related to the dispersion of reduced ruthenium in the zeolite. Methane selectivity, however, seems to be strongly influenced by the type and concentration of alkali cations remaining in the ion-exchanged zeolite. These cations appear to promote chain growth much as traditional alkali promoters would, though perhaps more indirectly. Due to bifunctional properties of the zeolite-supported catalysts, a significant fraction of C/sub 4/ was in the form of isobutane. Formation of isobutane seems to be related to either the Si/Al ratio in the zeolites or the concentration of the remaining alkali cations, but not to the OH concentration. In addition, H/sub 2/ chemisorption at 25/sup 0/C was increasingly ...

1984-02-01

438

RF effects on current-driven plasma instabilities

Wildland fire suppression personnel employed by the CDF do not currently have the equipment to protect themselves from the short-term acute affects of smoke from wildland fires. In addition, no regulations exist that specify appropriate respiratory protection and the current air-purifying respirator technology and carbon monoxide monitoring has not been adapted to fit wildland fire suppression requirements. This three-year limited study evaluated the ability of wildland fire fighters to perform their normal job function while wearing full-face air-purifying respirators. In the first two years of this study we designed, developed and field tested a prototype ``smart`` air-purifying respirator which incorporated a real-time carbon monoxide monitor into a commercial full-face respirator.` Data on carbon monoxide exposure while fighting wildland fires was collected. During the third year of this study we evaluated eight different commercially ...

1996-02-01

439

Photocurrent Noise in Quantum Dot Infrared Photodetectors

The Versatile Toroidal Facility (VTF) is a large laboratory plasma machine of 1 meter major radius used to carry out investigations of ionospheric plasma turbulence. Spectral analysis has been performed on plasmas produced by the electron emitters. Interest has focused on the low frequencies below the lower hybrid resonance where ion acoustic and current-convective modes have been observed. Microwaves injected from a 3,000 watt magnetron produce dramatic changes to the low frequency spectrum. First, the parametric decay instability intensifies the ion acoustic modes in the region of plasma heated by the microwaves. Second, the normally dominant current-convective modes are greatly suppressed in the heated region due to the oscillating electric field of the pump wave. When the authors probe beyond the heated region, these two pump wave effects are no longer observed, presumably because the microwaves are denied access to beyond the heated region due to the high ...

1996-12-31

440

MRI with SPIR sequences of optic nerve lesions; Utilita' delle sequenze 'Selective Partial Inversion Recover' (SPIR) nelle mallattie del nervo ottico

Low-frequency current noise and current-voltage (I-V) characteristics have been studied in InAs/GaAs self-assembled Quantum Dot Infrared Photodetectors in dark conditions and under illumination, at T = 77K and T = 5K. The noise behavior is consistent with a generation-recombination fluctuation process mainly related to thermally excited charge carriers at T = 77K. At T = 5K the current noise is consistent with a mechanism of fluctuations driven by the electric field, related to tunneling rather than emission-capture of charge carriers from the Quantum Dots. A very effective noise suppression mechanism, related to the tunneling regime, determines a decrease of fluctuation intensity as a function of the voltage. At T = 5K, an interesting behavior is observed in the current-voltage and noise power spectra for some of nominally identical QDIP structures in the presence of irradiation. Some devices indeed exhibit (i) a very high photoresponse and (ii) a 1/f-shaped noise ...

2005-08-01

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441

Long-term survivability of riprap for armoring uranium-mill tailings and covers: a literature review. [203 references

To evaluate the yield of SPIR sequences with fat suppression in the diagnosis of optic nerve lesions. T1 and T2 weighted fat suppressed SPIR imaging of the optic nerve improves anatomical definition, lesion detection and characterization in optic nerve conditions. [Italian] L'articolo analizza i vantaggi ricavabili dall'utilizzazione delle sequenze SPIR nella documentazione del decorso del nervo ottico e delle sue alterazioni nei pazienti con sospetto clinico e strumentale di malattia retrobulbare. L'utilizzo delle sequenze SPIR consente ottimale visualizzazione del nervo ottico e delle sue eventuali alterazioni senza gli artefatti che limitavano la qualita' delle immagini nelle prime sequenze RM con soppressione del segnale del grasso.

1999-04-01

442

Flash pyrolysis of coal-solvent slurry prepared from the oxidized coal and the coal dissolved in solvent; Ichibu yokaishita sanka kaishitsutan slurry no jinsoku netsubunkai

Pacific Northwest Laboratory (PNL) is investigating the use of a rock armoring blanket (riprap) to mitigate wind and water erosion of an earthen radon suppression cover applied to uranium mill tailings. Because the radon suppression cover and the tailings must remain intact for up to 1000 years or longer, the riprap must withstand natural weathering forces. This report is a review of information on rock weathering and riprap durability. Chemical and physical weathering processes, rock characteristics related to durability, climatic conditions affecting the degree and rate of weathering, and testing procedures used to measure weathering susceptibilities have been reviewed. Sampling and testing techniques, as well as analyses of physical and chemical weathering susceptibilities, are necessary to evaluate rock durability. Many potential riprap materials may not be able to survive 1000 years of weathering. Available techniques for durability ...

1982-06-01

443

Design experience of the JRR-2 BNCT facility in JAERI

In order to develop a high-efficiency coal pyrolysis method, flash pyrolysis was experimented on slurry prepared by using liquid-phase oxidation reformed coal and a methanol-based solvent mixture. Australian Morwell coal was used for the experiment. The oxidized coal, into which carboxyl groups have been introduced, has the condensation structure relaxed largely, and becomes highly fluid slurry by means of the solvent. Char production can be suppressed by making the oxidation-pretreated coal into slurry, resulting in drastically improved pyrolytic conversion. The slurry was divided into dissolved solution, dried substance, extracted residue, and residual slurry, which were pyrolized independently. The dissolved solution showed very high conversion. Improvement in the conversion is contributed by separating the dissolved substances (coal macromolecules) at molecular levels, coagulating the molecules, suppressing cross-link formation, and ...

1996-10-28

444

Defect suppression of indium end-of-range during solid phase epitaxy annealing using Si{sub 1-y}C {sub y} in silicon

The thermal neutron column of the JRR-2 reactor was remodelled in order to install a medical irradiation facility. The design experience and the cource of this remodelling are presented. At first, the thermal neutron flux was lower than expected, and the expected radiation condition was achieved by the following improvements: (1) removal of graphite from the thermal neutron column to increase the neutron flux, (2) twice increase of bismuth shielding and the shift of its position to improve the shielding effect against gamma ray, (3) application of B{sub 4}C rubber on the both surfaces of the shielding door to suppress the secondary gamma ray generation from the structural materials, (4) LiF tiles are applied on the inner face of the neutron beam exit hole to suppress the secondary gamma ray from the beam exit, and (5) installment a cone-shape polyethylene collimator to relieve the decay of thermal neutron. After the improvements, medical ...

1994-06-01

445

Complete suppression of Pfirsch-Schlueter current in a toroidal l=3 stellarator

We report on the elimination of defect formation which is associated with high dose indium implantations under solid phase epitaxial regrowth (SPER) annealing conditions of 650-800 deg. C. This is achieved by incorporating a layer of epitaxially grown Si{sub 1-y}C {sub y} layer, strategically located at the end-of-range (EOR) of the implant profile. An indium implant of 115 keV at 1 x 10{sup 14} cm{sup -2} was performed followed by annealing at temperature ranges of 650-800 deg. C. Samples with the Si{sub 1-y}C {sub y} layer revealed the elimination of secondary EOR defects with effectively suppressed indium transient enhanced diffusion (TED), indicating the function of carbon as an efficient sink for silicon interstitials at reduced annealing temperatures, in the SPER dopant activation regime.

2006-05-10

446

Carvedilol targets human K2P3.1 (TASK1) K+ leak channels

Pfirsch-Schlueter (P-S) current is an inherent property of a finite pressure toroidal equilibrium of tokamak and stellarator. However, it was pointed out recently (V.D. Pustovitov, Nuclear Fusion 36 (1996) 583) that the P-S current would be suppressed completely if the external vertical field could be adjusted to satisfy the condition {omega}=<{omega}> in an l=3 stellarator. Here {omega}=<B tilde{sup 2}>/B{sub 0}{sup 2}-2{epsilon} cos{theta}, l is a pole number, |B tilde| the vacuum helical magnetic field, B{sub 0} the toroidal field, {epsilon} the inverse aspect ratio, {theta} the poloidal angle and <...> denotes the average over the toroidal angle. An example of such a stellarator equilibrium is presented in this paper. For this stellarator equilibrium, behavior of rotational transform and Boozer magnetic spectrum is clarified when the pressure is increased. Both formation of helical magnetic axis and reduction of ...

1999-10-01

447

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND AND PURPOSE Human K2P3.1 (TASK1) channels represent potential targets for pharmacological management of atrial fibrillation. K2P channels control excitability by stabilizing membrane potential and by expediting repolarization. In the heart, inhibition of K2P currents by class III antiarrhythmic drugs results in action potential prolongation and suppression of electrical automaticity. Carvedilol exerts antiarrhythmic activity and suppresses atrial fibrillation following cardiac surgery or cardioversion. The objective of this study was to investigate acute effects of carvedilol on human K2P3.1 (hK2P3.1) channels. EXPERIMENTAL APPROACH Two-electrode voltage clamp and whole-cell patch clamp electrophysiology was used to record hK2P3.1 currents from Xenopus oocytes, Chinese hamster o...

2011-01-01

448

Using PCAR to study Cu/Co bilayers

Unitizations of double-ring structure and Erbium-doped waveguide amplifier for stable and tunable fiber laser

For spintronic applications it is important to establish how efficiently spins can be injected from a magnetic material into a non-magnetic material and the distance over which those spins survive. It is thought that spin polarised transport current can be determined by using the suppression of the Andreev reflection between a superconductor and the spin polarised material (Science 282 (1998) 85). Cu/Co bilayers are potentially an ideal test system for such study. In this paper we assess the feasibility of using point contact Andreev reflection spectroscopy to address this problem using a superconducting niobium tip at 4.2 K.

2004-05-01

449

British Library Electronic Table of Contents (United Kingdom)

We propose and demonstrate experimentally a singlelongitudinal-mode (SLM) fiber double-ring laser using an Erbium-doped waveguide amplifier (EDWA), polarization controller (PC), and a fiber Fabry-Perot tunable filter (FFP-TF) into the ring cavity. In addition, the output power, side-mode suppression ratio (SMSR), and the stabilities of power and wavelength of the laser also are investigated. (Copyright 2007 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA)

2007-01-01

450

Simulation of non-linear and switching elements for transient analysis based on wave digital filters

Red mud as a first stage catalyst in a two-stage close-coupled thermal catalytic hydroconversion process

A previous paper introduced the use of wave digital filters as a basic building block for power system simulation, particularly suitable for real-time applications. This paper stresses the simulation of non-linear and switching elements, emphasizing the advantages of the wave filters implementation. The digital structure is maintained even when non-linear components change their characteristics or power electronic devices switch their states. As a very important by-product, the suppression of numerical oscillations related to the trapezoidal rule is achieved in a rather simple way, with no effects on simulation results.

1996-10-01

451

Radiolysis of neutron irradiated lithium solutions

A process for the production of transportation fuels from heavy hydrocarbonaceous feedstock is provided comprising a two-stage, close-coupled process, wherein the first stage comprises a hydrothermal zone into which is introduced a mixture comprising a feedstock and red mud having coke-suppressing and demetalizing activity, and hydrogen; and the second, close-coupled stage comprises a hydrocatalytic zone into which substantially all the effluent from the first stage is directly passed and processed under hydrocracking conditions.

1985-12-17

452

Properties of A-15 superconductors with defects

In the Aqueous Self-Cooled Blanket (ASCB) concept, an aqueous "6Li solution in a metallic structure is used as a shielding-breeding blanket for fusion reactors. The experimental results of this study indicate that the radiolytic gas production in an ASCB will be proportional to the absorbed radiation energy. The observed radiation chemical yields allow the preliminary estimation of the radiolysis effects for a specific ASCB design. Contrary to the theoretical predictions, the use of hydrogen as a cover gas at up to 1 MPa had no measurable effect on the radiolytic gas production. Probably it will thus not be possible to suppress the radiolytic decomposition of a low-pressure ASCB by the addition of hydrogen. Catalytic recombination will be required. (orig.).

453

Production of four-weak-bosons and heavy Higgs signals in TeV photon-photon collisions

It is suggested that the large reduction of the superconducting transition temperature T sub(c) due to defects observed experimentally in some A-15 compounds is caused by smearing of a high peak in the density of states at the Fermi level. The influence of defects on other physical properties (the magnetic susceptibility chi, the elastic modulus C sub(s), the structural transformation temperature T sub(m) and the electrical resistivity rho) is also discussed from the same point of view. We expect the anomalous temperature dependence of chi C sub(s) and rho will be suppressed by defects. (auth.).

454

Precise measurement of theta_13 at Daya Bay

We have studied the signals for a heavy Higgs boson in the processes {gamma}{gamma}{yields}WWWW, and {gamma}{gamma}{yields}WWZZ at a photon linear collider. The results are based on the first complete tree-level calculation for these reactions. We show that, with a forward ``spectator`` W tag, and a central ``spectator`` W veto to suppress backgrounds from transverse W, Z production, the invariant mass spectrum of central WW, ZZ pairs is sensitive to Higgs bosons with a mass up to 1 TeV in a 2-TeV linear collider. ((orig.)).

1995-02-01

455

Possible influence of vacuum polarization on Q/sub 1//sub //sub s/ in muon catalyzed D-T fusion

The Daya Bay Reactor Neutrino Experiment is designed to determine the yet unknown neutrino mixing angle theta_13 by measuring the disappearance of electron antineutrinos from several nuclear reactor cores, using multiple underground detectors at different baselines to minimize systematic errors and to suppress the cosmogenic background. The civil construction has begun since October 2007, enabling first commissioning data in 2009, and full data taking will begin in late 2010. The planned sensitivity in sin^2 (2theta_13) of better than 0.01 at 90% CL will be achieved in three years of data-taking. I will present an overview and current status of the experiment.

2008-01-01

456

Photon-assisted spin-polarized transport in carbon nanotubes with impurities

The vacuum polarization splitting of the M-shell states in muonic hydrogen can have a profound influence on the muonit de-excitation cascade in deuterium and tritium targets. The cascade also shows sensitive dependence on the precise rate of transfer processes between certain excited muonic deuterium and tritium atoms. Recent experimental data, where a much greater population of the (d..mu..) qs state (1/sub 1//sub //sub s/) was found than previously predicted, can be explained if the transfer rates from the (d..mu..) M-shell are assumed to be strongly suppressed.

1988-12-27

457

Method and apparatus for efficient photodetachment and purification of negative ion beams

Impurity effects on the photon-assisted spin-polarized transport through armchair carbon nanotubes connected with ferromagnetic leads are investigated theoretically. The impurity induces one resonant state whose position depends on the impurity strength, which can break the electron-hole symmetry. Whether the impurity suppresses or enhances the spin-coherent current depends on the nanotube length. When the microwave fields are applied on the nanotube, additional small side peaks caused by the photon-assisted tunneling are found. With increasing the impurity strength, one new current peak appears under the influence of both the microwave fields and the impurity.

2006-12-25

458

Metals-impreganted red mud as a first-stage catalyst in a two-stage, close-coupled thermal catalytic hydroconversion process

Methods and apparatus are described for efficient photodetachment and purification of negative ion beams. A method of purifying an ion beam includes: inputting the ion beam into a gas-filled multipole ion guide, the ion beam including a plurality of ions; increasing a laser-ion interaction time by collisional cooling the plurality of ions using the gas-filled multipole ion guide, the plurality of ions including at least one contaminant; and suppressing the at least one contaminant by selectively removing the at least one contaminant from the ion beam by electron photodetaching at least a portion of the at least one contaminant using a laser beam.

2008-02-26

459

Magnetic and electrical properties of single crystalline Formula Not Shown

A process for the production of transportation fuels from heavy hydrocarbonaceous feedstock is provided comprising a two-stage, close-coupled process, wherein the first stage comprises a hydrothermal zone into which is introduced a mixture comprising a feedstock and metals-impregnated red mud having coke-suppressing and demetalizing activity, and hydrogen; and the second, close-coupled stage comprises a hydrocatalytic zone into which substantially all the effluent from the first stage is directly passed and processed under hydrocatalytic conditions. The preferred metals for impregnation include transition metals, in particular, nickel and molybdenum.

1985-12-17

460

British Library Electronic Table of Contents (United Kingdom)

We have successfully grown single crystalline Formula Not Shown with the range of Formula Not Shown using the floating-zone method. All compounds show orthorhombic symmetry in this substitution range, but the difference between lattice constants a and b decreases with increasing Sr concentration and becomes almost zero at Formula Not Shown . Characteristic temperatures, which correspond to antiferromagnetic ordering and structural transition, decrease with increasing Sr concentration. The value of the magnetic susceptibility below 30K increases with increasing Sr concentration. The temperature dependence of the electrical resistivity revealed that Sr substitution significantly suppresses the highly anisotropic electric structure of Formula Not Shown .

2008-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Lithium isotopes: differential effects in animals

Inhibition of Two-Photon Absorption in a Four-Level Atomic System with Closed-Loop Configuration

Differences in the biological disposition and in the effects of the stable lithium isotopes, Li-6 and Li-7, in animals were characterized. A somewhat higher toxicity was associated with WLiCl compared to XLiCl with NLiCl intermediate. All lithium treatments increased water intake and resulted in weight gain. Peak plasma concentration was slightly high for 6Li than 7LiCl suppressed action potential amplitudes during tetanic stimulation more than NaCl when added to Renger solution bathing ganglia.

1984-04-01

462

Genes involved in immortalization of human mammary cells

We theoretically investigate the features of two-photon absorption in a coherently driven four-level atomic system with closed-loop configuration. It is found that two-photon absorption can be completely suppressed just by properly adjusting the relative phase of four coherent low-intensity driving fields and the atomic system becomes transparent against two-photon absorption. From a physical point of view, we explicitly explain these results in terms of quantum interference induced by two different two-photon excitation channels.

2007-05-15

463

Experimental study on fuzzy logic vibration control of a bridge using fail-safe magnetorheological fluid dampers

Breast cancer progression is characterized by inappropriate cell growth. Normal cells cease growth after a limited number of cell divisions--a process called cellular senescence-while tumor cells may acquire the ability to proliferate indefinitely (immortality). Inappropriate expression of specific oncogenes in a key cellular signaling pathway (Ras, Raf) can promote tumorigenicity in immortal cells, while causing finite lifespan cells to undergo a rapid senescence-like arrest. We have studied when in the course of transformation of cultured human mammary epithelial cells (HMEC), the response to overexpressed oncogenic Raf changes from being tumor-suppressive to tumor enhancing, and what are the molecular underpinnings of this response. Our data indicate: (1) HMEC acquire the ability to maintain growth in the presence of oncogenic Raf not simply as a consequence of overcoming senescence, but as a result of a newly discovered step in the process of immortal ...

2001-09-27

464

Effect of secondary circuit materials and water regime on steam generator reliability

This study presents a semi-active vibration control of a scaled two-span bridge structure. Magneto-rheological fluid dampers are utilized as the semi-active energy absorbing devices, and a bridge vibration control system is developed. Closed-loop control system based on fuzzy logic is used to suppress the bridge deck motion under random excitation. It is demonstrated that this fuzzy logic control system can significantly reduce the relative deck displacement using about 60% less power compared to passive on state, while the absolute deck acceleration remains practically unchanged.

2001-07-01

465

Effect of a diluent on the performance of a hydrogen-diesel dual-fuel engine

The mechanism of the salt concentration increase in pits and crevices formed in a steam generator due to its imperfect manufacture or to its design features is described. The probability of corrosion can be reduced by choosing a suitable steel and by securing low concentrations of salts (chlorides in particular) and corrosion products in the feedwater. Attention is paid to the distribution of salts in the water-steam circuit and to the conditions of erosion corrosion as the principal source of corrosion products in feedwater. Experience with the suppression of erosion corrosion at nuclear power plants abroad is described. (E.J.).

1989-05-01

466

Diagnosing hepatocellular carcinoma with the intensity and the lifetime of two-photon red autofluorescences

Earlier work carried out on the hydrogen-diesel dual-fuel engine indicates that knock sets in when the inducted hydrogen exceeds about 60% of input energy with a pilot quantity of 30% of full load diesel amount. Some of the findings of an investigation into knock-free performance of a hydrogen-diesel dual-fuel engine with the addition of a diluent-carbon dioxide are presented. The effects of carbon dioxide on the suppression of knocking in the hydrogen-diesel dual-fuel engine and on the improvement in the knock limited power output are described.

1986-11-01

467

Decaying into the Hidden Sector

We demonstrated that the intensity levels and lifetimes of two-photon autofluorescences (2PAF) in human liver tissues can be exploited to diagnose hepatocellular carcinoma (HCC). Excited by an infrared femtosecond laser, we suppressed the two-photon autofluorescences of most endogenous fluorophores and made red autofluorescences more specific to particular molecules in the cryo-sectioned human livers. Using such fluorescence contrast, we found HCC tissues have reduced levels of 2PAF and longer fluorescence lifetime.

2011-02-01

468

Computational analysis of the oscillatory dynamics in the processes of CO2 assimilation and photorespiration

The existence of light hidden sectors is an exciting possibility that may be tested in the near future. If DM is allowed to decay into such a hidden sector through GUT suppressed operators, it can accommodate the recent cosmic ray observations without over-producing antiprotons or interfering with the attractive features of the thermal WIMP. Models of this kind are simple to construct, generic and evade all astrophysical bounds. We provide tools for constructing such models and present several distinct examples. The light hidden spectrum and DM couplings can be probed in the near future, by measuring astrophysical photon and neutrino fluxes. These indirect signatures are complimentary to the direct production signals, such as lepton jets, predicted by these models.

2009-01-01

469

British Library Electronic Table of Contents (United Kingdom)

The computational analysis of the model system consisting of the processes of CO2 assimilation and photorespiration shows the appearance of sustained oscillations in the system which might reflect their presence in photosynthesizing cells. Concentrations of CO2 and O2 oscillate in opposite phases causing Rubisco switching continuously between the carboxylase (CO2 assimilation) and the oxygenase (photorespiration) reactions. The results of modeling are consistent with carbon isotopic and other observed data. They show that the oscillation period varies from about 1 s to 3 s depending on the values of parameters taken. Too high concentrations of O2 suppress the oscillations.

2011-01-01

470

Asymmetric fingered TFT structure: a new architecture for Kink effect and off-current suppression and improved stability

Application of high energy ion beam for the control of boron diffusion

The asymmetric fingered structure for polysilicon thin-film transistors (AF-TFTs) is analysed in detail by combining experimental characteristics and two-dimensional numerical simulations. This structure allows an effective reduction of the kink effect and off-current, without introducing any additional series resistance. In addition, a substantial improvement in the device stability is also observed when compared to conventional TFT. The AF-TFT characteristics have been explained by considering a two transistor model.

2006-01-01

471

Application of high energy ion beam for the control of boron diffusion

For the purpose of optimizing the process of co-implantation of MeV Si ions to reduce boron transient enhanced diffusion and boron-enhanced diffusion in Si, multiple MeV implantations and annealing at different temperatures have been performed. A slight improvement on the suppression of B diffusion is observed by adding a low temperature annealing step after the MeV implantation. No differences in B diffusion are observed when the Si doses are increased from 1 x 10{sup 15} to 1 x 10{sup 16} cm{sup -2}. This dose independent behavior is speculated to be a quasi-steady state of vacancy cluster evaporation.

2006-01-15

472

Analysis of the pressure variation phenomena during condensation oscillation and chugging

For the purpose of optimizing the process of co-implantation of MeV Si ions to reduce boron transient enhanced diffusion and boron-enhanced diffusion in Si, multiple MeV implantations and annealing at different temperatures have been performed. A slight improvement on the suppression of B diffusion is observed by adding a low temperature annealing step after the MeV implantation. No differences in B diffusion are observed when the Si doses are increased from 1 x 10"1"5 to 1 x 10"1"6 cm"-"2. This dose independent behavior is speculated to be a quasi-steady state of vacancy cluster evaporation.

2006-01-01

473

An overview of standards concerning unwanted electrostatic discharges

To get a better understanding of the condensation oscillation and chugging process during blowdown of a BWR after a LOCA a large number of large scale pressure suppression experiments have been performed in several countries. ECN has performed a detailed analysis of the results of one of these experiments, e.g. the DAS M10 experiment of GKSS. This analysis includes the following aspects: Power Spectral Density (PSD) analysis of the data, analysis of the acoustic frequencies of the downcomers and an analysis of the eigenfrequencies of the downcomers. The results of these analyses and an evaluation are given in this paper. It is shown that all dominant frequencies in the experimental results can be correlated with the vent-acoustic or eigenfrequencies. (orig.).

1985-08-01

474

British Library Electronic Table of Contents (United Kingdom)

For users and manufacturers affected by unwanted electrostatic discharges, the publication of standards concerning the control of such discharges is a great improvement and helps to prevent unwanted electrostatic discharges in potentially explosive atmospheres as well as around electrostatically sensitive devices. There are, for instance, handbook-like documents giving recommendations to help set up effective electrostatic suppressing measures and programs. Another group of standards focuses on certain products and processes and is, thus, more specific. A third group deals with test methods and specifications to help manufacturers develop products that are safe. It also helps in comparisons with other products and thus meets the customer's real needs. To assist manufacturers and customers ...

2009-01-01

475

Acquired cystic kidney disease

Acceleration Control in Nonlinear Vibrating Systems based on Damped Least Squares

Acquired cystic kidney disease (ACKD), also known as acquired renal cystic disease (ARCD,) occurs in patients who are on dialysis for end-stage renal disease. It is generally accepted that ACKD develops as a consequence of sustained uremia and can first manifest even before dialysis is initiated while the patient is still in chronic renal failure. The role of immune suppression, particularly in transplant recipients, in the development of ACKD, is still under investigation. The prevalence of ACKD is directly related to the duration of dialysis and the risk of cancer is directly related to the presence of cysts. Herein we review the current understanding of the pathophysiology and imaging implications of ACKD. (orig.)

2000-11-01

476

Use of evaporation for the treatment of liquids in the nuclear industry

A discrete time control algorithm using the damped least squares is introduced for acceleration and energy exchange controls in nonlinear vibrating systems. It is shown that the damping constant of least squares and sampling time step of the controller must be inversely related to insure that vanishing the time step has little effect on the results. The algorithm is illustrated on two linearly coupled Duffing oscillators near the 1:1 internal resonance. In particular, it is shown that varying the dissipation ratio of one of the two oscillators can significantly suppress the nonlinear beat phenomenon.

2011-01-01

477

The Relationship of Vegetation and Environmental Factors in Wangsuk Stream and Gwarim Reservoir: I. Water Environments

... The results show that a system decontamination factor of 10^ to 10* can be

1994-07-25

478

The Factors for the Formation of Carcinogenic N-Nitrosamine from Dried Marine Food Products

Full Text Available

2005-12-01

479

Study of the Form Factor of the Proton in the Timelike Region for Large Momentum Transfers

Full Text Available

1994-05-01

480

Prognostic factors for the success of laser iridotomy for acute primary angle closure glaucoma.

No abstract prepared.

2001-01-01

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481

Potentiation of Antitumor Effect of Radiotherapy by Recombinant Tumor Necrosis Factor-$\\alpha$

Full Text Available

2009-12-01

482

Optimizations of 3D MRI Techniques in Brain by Evaluating SENSE Factors

Full Text Available

1998-09-01

483

Is smoking a risk factor for decreased semen quality? A cross-sectional analysis.

Full Text Available

2009-12-01

484

Investigation of the Performance Based Structural Safety Factor of Elbows in Nuclear Power Plants

PMID:16966350

2006-09-11

485

Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary.

Full Text Available

2009-08-01

486

Germination and Seedling Emergence of Ammannia coccinea as Influenced by Environmental Factors

Udgivelsesdato: 2008-Jul-15

2008-01-01

487

Forecasting regional house price inflation: a comparison between dynamic factor models and vector autoregressive models

Full Text Available

2010-06-01

488

CSIR Research Space (South Africa)

Full Text Available

489

Factors of Cardiopulmonary Resuscitation Outcome for In-hospital Adult Patients

Evaluation of Ocular Risk Factors Related to Asymmetric Visual Field Defects in Normal Tension Glaucoma

Full Text Available

2007-12-01

490

Environmental Effects on Oil Pipelines

Full Text Available

2008-09-01

491

International Science & Technology Center (ISTC)

Determination of the Ecology Factor for the Magistral Oil Pipelines, Working in Difficult Hilly and Geology Conditions

492

Cholesterol Goal Attainment Rate and Its Associated Factors Among Dyslipidemic Patients on Lipid-lowering Drug Therapy in Korea

Analysis of the relationship between metabolic parameters and the low doses irradiation and non-raditaion factors in humans

Dyslipidemia

2011-09-06

493

An In-Hospital Family Member Cardiopulmonary Resuscitation (CPR) Education Program

Short communication.

1994-10-25

494

A prospective study on the incidence and predictive factors of relative adrenal insufficiency in Korean critically-ill patients.

Cardiac Arrest; Coronary Disease; Cardiovascular Risk Factors

2010-12-13

495

A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism.

Full Text Available

2009-08-01

496

A Numerical Study on Slip Factor Variations in Centrifugal Compressor Impellers

Transcription activation of sulfur metabolism in yeast is dependent on two DNA binding factors, the centromere binding factor 1 (Cbf1) and Met4. While the role of Met4 was clearly established by showing...Full Text Available

1996-05-15

497