facilitates chromatin transcription: Topics by WorldWideScience.org

Epitope tagging of endogenous proteins for genome wide Chromatin immunoprecipitation analysis

BackgroundChromatin insulators or boundary elements are a class of functional elements in the eukaryotic genome. They regulate gene transcription by interfering with promoter-enhancer...Full Text Available

2

Acetylation of Histones and Transcription-Related Factors

The development of chromatin immunoprecipitation methods coupled with DNA microarray (ChIP-chip) technology has enabled genome-wide identification of cis-DNA regulatory elements to which transcription...Full Text Available

2009-01-01

3

c-Myc Mediates a Hypoxia-Induced Decrease in Acetylated Histone H4

The state of chromatin (the packaging of DNA in eukaryotes) has long been recognized to have major effects on levels of gene expression, and numerous chromatin-altering strategies—including...Full Text Available

2000-06-01

4

The dimerization domain of SOX9 is required for transcription activation of a chondrocyte-specific chromatin DNA template

Global acetylation of histone H4 is a mark of gene transcriptional activation. The c-Myc transcription factor binds to specific DNA sites in cellular chromatin and induces the acetylation of...Full Text Available

2009-10-01

5

Replication timing and transcriptional control: beyond cause and effect. Part II

Mutations in SOX9, a gene essential for chondrocyte differentiation cause the human disease campomelic dysplasia (CD). To understand how SOX9 activates transcription, we characterized...Full Text Available

2010-10-01

6

Inferring transcription factor complexes from ChIP-seq data

SUMMARYReplication timing is frequently discussed superficially in terms of its relationship to transcriptional activity via chromatin structure. However, so little is known about...Full Text Available

2009-04-01

7

Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging-Related Transcriptional Networks

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) allows researchers to determine the genome-wide binding locations of individual transcription factors (TFs) at high resolution....Full Text Available

2011-08-01

8

Structural Basis for Acetylated Histone H4 Recognition by the Human BRD2 Bromodomain*

The sirtuin Sirt6 is a NAD-dependent histone deacetylase that is implicated in gene regulation and lifespan control. Sirt6 can interact with the stress-responsive transcription factor NF-κB...Full Text Available

2011-06-01

9

Monovalent and unpoised status of most genes in undifferentiated cell-enriched Drosophila testis

Recognition of acetylated chromatin by the bromodomains and extra-terminal domain (BET) family proteins is a hallmark for transcriptional activation and anchoring viral genomes to mitotic chromosomes...Full Text Available

2010-03-05

10

The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin.

BackgroundIncreasing evidence demonstrates that stem cells maintain their identities by a unique transcription network and chromatin structure. Opposing epigenetic modifications...Full Text Available

2010-01-01

11

Influence of chromatin molecular changes on RNA synthesis during embryonic development

The products of trithorax group (trxG) genes maintain active transcription of many important developmental regulatory genes, including homeotic genes. Several trxG proteins have been shown to act in...Full Text Available

2004-07-01

12

Quantitation of transcribing native simian virus 40 minichromosomes extracted from CV1 cells late in infection.

Two aspects of the chromatin repeat length (r_l) are discussed: (i) Why is r_l longer for slowly dividing cells than in rapidly dividing cells?, and (ii) Why is the temporal evolution of r_l a decreasing function of time (t) in mammalian cortical neurons whereas it is an increasing function of t for granule cells around the time of birth? These questions are discussed in terms of a hypothesis which assumes a correlation between deoxyribonucleic acid (DNA) packaging, transcription, and replication. (author). 27 refs.

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A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors

Simian virus 40 transcriptional complexes could be extracted from CV1 cells late in infection and separated from the bulk of inactive viral chromatin. Sucrose gradient sedimentation, cesium sulfate...Full Text Available

1981-04-01

14

Transcriptional regulation in Drosophila: the post-genome challenge.

Transcriptional silencing in Saccharomyces cerevisiae occurs at several genetic loci, including the ribosomal DNA (rDNA). Silencing at telomeres (telomere position effect [TPE])...Full Text Available

1999-04-01

15

Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography.

Drosophila melanogaster has long been at the forefront of studies of transcriptional regulation in animals. Many fundamental ideas--such as cis control elements that act over long distances, the regulation of development by hierarchical cascades of transcription factors, dosage compensation, and position effect variegation--originated from studies of the fruit fly. The recent completion of the euchromatic DNA sequence of Drosophila is another breakthrough. The sequence data highlight important unanswered questions. For example, only one-fifth of the 124 Mb of Drosophila euchromatic DNA codes for protein. The function of the remaining 100 Mb of mostly unique DNA is largely unknown. Some proportion of this non-reading frame DNA must encode the functional recognition sites targeted by the approximately 700 sequence-specific DNA binding proteins that regulate transcription in Drosophila, but what proportion? Most or very ...

2001-03-01

16

Transcription Factor IIA t Is Associated with Undifferentiated Cells and Its Gene Expression Is Repressed in Primary Neurons at the Chromatin Level In Vivo

We recently found that many RNA polymerase II transcription factors are modified with N-acetylglucosamine residues. These sugar moieties confer upon transcription factors an ability to bind the lectin wheat germ agglutinin. We have taken advantage of this interaction to devise a purification procedure for the "GC-box" binding transcription factor Sp1. Crude nuclear extracts are first subjected to wheat germ agglutinin affinity chromatography and then subjected to sequence-specific DNA affinity chromatography. The Sp1 protein purified by this procedure is at least 95% pure, and the overall recovery is greater than 80%. In addition to yielding larger quantities of Sp1 than conventional schemes, the new purification procedure is also simpler and more rapid. We show that wheat germ agglutinin affinity chromatography can also be used to purify the glycosylated forms of the CCAAT-binding transcription factor. ...

1989-03-01

17

British Library Electronic Table of Contents (United Kingdom)

The levels of General Transcription Factor (TF) IIA were examined during mammalian brain development and in rat embryo fibroblasts and transformed cell lines. The large TFIIA subunit paralogues ab and t are largely produced in unsynchronized cell lines, yet only TFIIA ab is observed in a number of differentiated tissue extracts. Steady-state protein levels of the TFIIA t, ab, and g subunits were significantly reduced when human embryonal (ec) and hepatic carcinoma cell lines were stimulated to differentiate with either all-trans-retinoic acid (ATRA) or sodium butyrate. ATRA-treated NT2-ec cells required replating to induce a neuronal phenotype and loss of detectable TFIIA t and g proteins. High levels of TFIIA t, ab, and g and Sp factors were identified in extracts from human fetal and rat...

2006-01-01

18

Butyrate Induces Expression of 17?-Hydroxysteroid Dehydrogenase Type 1 in HT29 and SW707 Colorectal Cancer Cells

British Library Electronic Table of Contents (United Kingdom)

Epidemiological studies have revealed that butyrate and 17?-estradiol (E2) may decrease the incidence of colorectal cancer (CRC). In peripheral tissue, E2 can be produced locally by 17?-hydroxysteroid dehydrogenase 1 (HSD17B1) estrone (E1) reduction. Using quantitative real-time polymerase chain reaction and western blotting analysis, we found that sodium butyrate significantly upregulates HSD17B1 long and short transcripts and protein levels in HT29 and SW707 CRC cells. Chromatin immunoprecipitation analysis showed that upregulation of these transcript levels correlated with an increase in binding of Polymerase II to proximal and distal promoters of HSD17B1. Moreover, we observed that upregulation of HSD17B1 protein levels was associated with increased conversion of E1 to E2 in HT29 and S...

2011-01-01

19

Gene Repressive Activity of RIP140 Through Direct Interaction with CDK8.

Structure of plant nuclear and ribosomal DNA containing chromatin.

Receptor interacting protein 140 (RIP140) is a coregulator for numerous nuclear receptors and transcription factors and primarily exerts gene-repressive activities on various target genes. We previously identified a spectrum of posttranslational modifications on RIP140 that augment its property and biological activity. In T(3)-triggered biphasic regulation of cellular retinoic acid binding protein 1 (Crabp1) gene along the course of fibroblast-adipocyte differentiation, we found TRAP220(MED1) critical for T(3)-activated chromatin remodeling whereas RIP140 essential for T(3)-repressive chromatin remodeling of this gene promoter. In this current study, we aim to examine whether and how RIP140 replaces TRAP220(MED1) on the CrabpI promoter in differentiating adipocyte cultures. We find increasing recruitment of RIP140 to this promoter, with corresponding reduction in TRAP220(MED1) recruitment during the T(3)-repressive phase. ...

2011-08-25

20

Centromere-encoded RNAs are integral components of the maize kinetochore

Digestion of plant chromatin from Brassica pekinensis and Matthiola incana with staphylococcus nuclease leads to a DNA repeat of 175 plus or minus 8 and a core size of 140 base pairs. DNase I digestion...Full Text Available

1979-11-10

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A statistical framework for modeling gene expression using chromatin features and application to modENCODE datasets

RNA is involved in a variety of chromatin modification events, ranging from large-scale structural rearrangements to subtle local affects. Here, we extend the evidence for RNA–chromatin interactions...Full Text Available

2004-11-09

22

The influence of treatment with thiotepa, thyroxine and D3 vitamin and the effect of fast neutron radiolysis on walker tumor chromatin

We develop a statistical framework to study the relationship between chromatin features and gene expression. This can be used to predict gene expression of protein coding genes, as well as microRNAs....Full Text Available

2011-01-01

23

TMPRSS6 rs855791 modulates hepcidin transcription in vitro and serum hepcidin levels in normal individuals.

Anticancer drug thyotepa (10 mg/ kg) hormonal compound thyroxine (40 mg / kg) and D3 vitamin (30,000 IU / kg) have been administrated simply or associated to Wistar rats bearing Walker carcinosarcoma. The chromatin (the complex of DNA and proteins from nuclei) has been extracted from Walker tumor and submitted to fast neutron beams produced by deuterons (13 MeV) on thick Be target at an IPNE U-120 Cyclotron, in doses of 5-100 Gy. Thermal transition of chromatin fluorescence intensity of chromatin-ethidium bromide complexes and intrinsic fluorescence of chromatin have been analysed. Association of thiotepa with thyroxine and D3 vitamin produced a diminution of chromatin lesions induced by the cytostatic. Thus in the effects of fast neutrons radiolysis in chromatin significant differences occurred. These results could help to improve the methodology of associated ...

1995-12-02

24

Molecular studies of the uncoupling protein

The iron hormone hepcidin is inhibited by matriptase-2, a liver serine-protease encoded by TMPRSS6 gene. Cleaving the BMP-coreceptor hemojuvelin, matriptase-2 impairs the BMP/SMAD signaling pathway, downregulates hepcidin and facilitates iron absorption. TMPRSS6 inactivation causes iron-deficiency-anemia refractory to iron administration both in humans and mice. Genome wide association studies have shown that the SNP rs855791, which causes the matriptase-2 V736A amino acid substitution, is associated with variations of serum iron, transferrin saturation, hemoglobin and erythrocyte traits. Here we show that in vitro matriptase-2 736(A) inhibits hepcidin more efficiently than 736(V). Moreover, in a genotyped population, after exclusion of samples with iron deficiency and inflammation, hepcidin, hepcidin/transferrin saturation and hepcidin/ferritin ratios were significantly lower and iron parameters were consistently higher in homozygotes 736(A) than in 736(V). Our ...

2011-08-26

25

Facilitator control as automatic behavior: A verbal behavior analysis

The uncoupling protein (UCP) is a proton/anion transporter found in the inner mitochondrial membrane of brown adipocyte. Although UCP has nor been detected in mitochondria from any other tissue, it shares structural and catalytic properties with several other mitochondrial carrier proteins. Although UCP was discovered only recently it is one of the most extensively studied mitochondrial carrier proteins.More recently, the mouse, rat, and human genes encoding for UCP have been isolated and sequenced. The availability of these various tools has led to several significant observations. UCP gene expression is strongly controlled at the level of transcription by signals that are activated after the stimulation of brown adipocytes by norepinephrine. The comparison of UCP gene with the genes encoding the adenine nucleotide translocator revealed the existence of structural and evolutionary homologies. Moreover, in humans the UCP gene and one form of adenine nucleotide ...

1991-06-01

26

UHRF1, a modular multi-domain protein, regulates replication-coupled crosstalk between DNA methylation and histone modifications

Several studies of facilitated communication have demonstrated that the facilitators were controlling and directing the typing, although they appeared to be unaware of doing so. Such results shift...Full Text Available

1993-01-01

27

SirT1 brings stemness closer to cancer and aging

Cytosine methylation in DNA is a major epigenetic signal, and plays a central role in propagating chromatin status during cell division. However the mechanistic links between DNA methylation...Full Text Available

2009-01-01

28

Identification of a Novel Inhibitor of Coactivator-associated Arginine Methyltransferase 1 (CARM1)-mediated Methylation of Histone H3 Arg-17*

Sirtuin 1 acts in various cell processes, deacetylating both chromatin and non-histone proteins, and its role in cancer and aging has long been studied and debated. Here we discuss another aspect of...Full Text Available

29

Arabidopsis thaliana Chromosome 4 Replicates in Two Phases That Correlate with Chromatin State

Methylation of the arginine residues of histones by methyltransferases has important consequences for chromatin structure and gene regulation; however, the molecular mechanism(s) of methyltransferase...Full Text Available

2010-03-05

30

A Novel Function of the DNA Repair Gene rhp6 in Mating-Type Silencing by Chromatin Remodeling in Fission Yeast

DNA replication programs have been studied extensively in yeast and animal systems, where they have been shown to correlate with gene expression and certain epigenetic modifications. Despite the conservation...Full Text Available

2010-06-01

31

Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression of forkhead transcription factor and atrophy gene transcription

Recent studies have indicated that the DNA replication machinery is coupled to silencing of mating-type loci in the budding yeast Saccharomyces cerevisiae, and a similar silencing mechanism...Full Text Available

1998-09-01

32

Direct Evidence for Postmeiotic Transcription During Drosophila melanogaster Spermatogenesis

Cellular hypertrophy is regulated by coordinated pro- and antigrowth machineries. Foxo transcription factors initiate an atrophy-related gene program to counter hypertrophic growth. This study was designed...Full Text Available

2008-09-01

33

The IKK complex contributes to the induction of autophagy

Extensive gene expression during meiosis is a hallmark of spermatogenesis. Although it was generally accepted that RNA transcription ceases during meiosis, recent observations suggest that some transcription...Full Text Available

2010-09-01

34

Global Transcriptional Responses of Fission Yeast to Environmental Stress

In response to stress, cells start transcriptional and transcription-independent programs that can lead to adaptation or death. Here, we show that multiple inducers of autophagy, including nutrient...Full Text Available

2010-02-03

35

Specific in vitro initiation of transcription on the adenovirus type 2 early and late EII transcription units.

We explored transcriptional responses of the fission yeast Schizosaccharomyces pombe to various environmental stresses. DNA microarrays were used to characterize changes in expression...Full Text Available

2003-01-01

36

Protein-protein interactions and structural analysis of citrus proteins involved in transcriptional control during citrus canker disease

Three transcription units are present in the adenovirus type 2 region EII. Transcription units EIIaE and EIIaL encode the mRNA for the 72,000-dalton DNA binding protein, early and late in the lytic...Full Text Available

1981-12-01

37

Post-transcriptional and post-translational regulation during mouse oocyte maturation

No abstract prepared.

2010-07-01

38

KoreaScience (Korea)

Full Text Available

2011-03-01

39

Acclimation of a terrestrial plant to submergence facilitates gas exchange under water

DEFF Research Database (Denmark)

Udgivelsesdato: October 2004

2004-01-01

40

Transcriptional profile of isoproterenol-induced cardiomyopathy and comparison to exercise-induced cardiac hypertrophy and human cardiac failure

Transcriptional Organization of the Avian Adenovirus CELO

BackgroundIsoproterenol-induced cardiac hypertrophy in mice has been used in a number of studies to model human cardiac disease. In this study, we compared the transcriptional response...Full Text Available

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41

Transcription induces strand-specific mutations at the 5? end of human genes

A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs)....Full Text Available

1998-11-01

42

Time-Dependent Expression of Arc and Zif268 after Acquisition of Fear Conditioning

A regional analysis of nucleotide substitution rates along human genes and their flanking regions allows us to quantify the effect of mutational mechanisms associated with transcription in germ line...Full Text Available

2008-08-01

43

TFIIH Operates through an Expanded Proximal Promoter To Fine-Tune c-myc Expression

Memory consolidation requires transcription and translation of new protein. Arc, an effector immediate early gene, and zif268, a regulatory transcription factor, have been implicated in synaptic plasticity...Full Text Available

2010-01-01

44

Regulation of human ribosomal RNA transcription.

A continuous stream of activating and repressing signals is processed by the transcription complex paused at the promoter of the c-myc proto-oncogene. The general transcription factor...Full Text Available

2005-01-01

45

RNA polymerase II trigger loop residues stabilize and position the incoming nucleotide triphosphate in transcription

We have used a cell-free polymerase I transcription system derived from HeLa cells to study the regulation of human rRNA synthesis. Analysis of deletion mutants spanning the start site of transcription at nucleotide +1 indicates that the control region affecting initiation of human rRNA synthesis is contained within sequences from nucleotides -158 to +18. This promoter region can be subdivided into (i) a central segment of approximately 40 base pair that is required for transcription and (ii) flanking sequences that influence the efficiency of transcription in vitro. We have examined the in vitro transcriptional activity of the human extract under various conditions that are thought to modulate rRNA synthesis in vivo. Cell-free extracts prepared from HeLa cells infected with adenovirus 2 synthesize human rRNA at levels greatly decreased relative to uninfected cell extracts. By ...

1983-06-01

46

Nuclear Receptor Rev-erb Alpha (Nr1d1) Functions in Concert with Nr2e3 to Regulate Transcriptional Networks in the Retina

A structurally conserved element, the trigger loop, has been suggested to play a key role in substrate selection and catalysis of RNA polymerase II (pol II) transcription elongation. Recently resolved...Full Text Available

2010-09-07

47

Molecular basis of FIR-mediated c-myc transcriptional control

The majority of diseases in the retina are caused by genetic mutations affecting the development and function of photoreceptor cells. The transcriptional networks directing these processes are regulated...Full Text Available

48

Modeling RNA polymerase competition: the effect of ?-subunit knockout and heat shock on gene transcription level

The Far UpStream Element (FUSE) regulatory system promotes a peak in the concentration of c-Myc during cell cycle. First, the FBP transcriptional activator binds to the FUSE DNA element upstream...Full Text Available

2010-09-01

49

Minigenome-Based Reporter System Suitable for High-Throughput Screening of Compounds Able To Inhibit Ebolavirus Replication and/or Transcription ?

BackgroundModeling of a complex biological process can explain the results of experimental studies and help predict its characteristics. Among such processes is transcription in...Full Text Available

50

MAPPING THE INITIATOR BINDING TAF2 SUBUNIT IN THE STRUCTURE OF HYDRATED YEAST TFIID

We describe an Ebolavirus minigenome-based system that is suitable for high-throughput screening of compounds able to impair Ebolavirus virus replication and/or transcription....Full Text Available

2010-07-01

51

Localizing potentially active post-transcriptional regulations in the Ewing's sarcoma gene regulatory network

SummaryThe general transcription factor TFIID is a large multi-subunit complex required for the transcription of most protein-encoding genes by RNA polymerase II. Taking advantage...Full Text Available

2009-03-11

52

Identification of inhibitors of auxin transcriptional activation by means of chemical genetics in Arabidopsis

BackgroundA wide range of techniques is now available for analyzing regulatory networks. Nonetheless, most of these techniques fail to interpret large-scale transcriptional data...Full Text Available

53

Heat Stress Enhances the Accumulation of Polyadenylated Mitochondrial Transcripts in Arabidopsis thaliana

Auxin modulates diverse plant developmental pathways through direct transcriptional regulation and cooperative signaling with other plant hormones. Genetic and biochemical approaches have clarified...Full Text Available

2004-10-12

54

Genome-Wide Identification and Evolutionary Analysis of the Animal Specific ETS Transcription Factor Family

BackgroundPolyadenylation of RNA has a decisive influence on RNA stability. Depending on the organisms or subcellular compartment, it either enhances transcript stability or targets...Full Text Available

55

FoxO Transcription Factors in Brain: Regulation and Behavioral Manifestation

The ETS proteins are a family of transcription factors (TFs) that regulate a variety of biological processes. We made genome-wide analyses to explore the classification of the ETS gene family. We identified...Full Text Available

56

Elk-1 a Transcription Factor with Multiple Facets in the Brain

BackgroundThe mammalian FoxO transcription factors function to regulate diverse physiological processes. Emerging evidence that both BDNF and lithium suppress FoxO...Full Text Available

2009-01-15

57

Dual Roles of Nrf2 in Cancer

The ternary complex factor (TCF) Elk-1 is a transcription factor that regulates immediate early gene (IEG) expression via the serum response element (SRE) DNA consensus site. Elk-1 is associated with...Full Text Available

58

Defective hepatitis B virus particles are generated by packaging and reverse transcription of spliced viral RNAs in vivo.

In response to oxidative stress, the transcription factor NF-E2-related factor 2 (Nrf2) controls the fate of cells through transcriptional upregulation of antioxidant response element (ARE)-bearing...Full Text Available

2008-01-01

59

Comparative Transcriptional and Genomic Analysis of Plasmodium falciparum Field Isolates

Generation of replicative defective viruses is frequently observed during viral infections. We now report that encapsidation and reverse transcription of spliced viral RNA is an additional mechanism...Full Text Available

1991-10-01

60

Combinatorial Gene Regulation Using Auto-Regulation

Mechanisms for differential regulation of gene expression may underlie much of the phenotypic variation and adaptability of malaria parasites. Here we describe transcriptional variation among culture-adapted...Full Text Available

2009-10-01

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61

Chronic Cocaine-Induced H3 Acetylation and Transcriptional Activation of CaMKII? in the Nucleus Accumbens Is Critical for Motivation for Drug Reinforcement

As many as 59% of the transcription factors in Escherichia coli regulate the transcription rate of their own genes. This suggests that auto-regulation has one or more important...Full Text Available

2010-06-01

62

A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

The regulation of gene expression in the brain reward regions is known to contribute to the pathogenesis and persistence of drug addiction. Increasing evidence suggests that the regulation of gene transcription...Full Text Available

2010-03-01

63

A Novel Form of Transcriptional Silencing by Sum1-1 Requires Hst1 and the Origin Recognition Complex

We have previously shown that a T to G transversion at the second T of the conalbumin "TATA" box drastically decreases specific initiation of transcription by RNA polymerase B (1). We now report that...Full Text Available

1981-04-24

64

Specific requirement of the chromatin modifier mSin3B in cell cycle exit and cellular differentiation

In the yeast Saccharomyces cerevisiae, a and α mating-type information is stored in transcriptionally silenced cassettes called HML and HMR....Full Text Available

2001-05-01

65

SWI/SNF and Asf1 Independently Promote Derepression of the DNA Damage Response Genes under Conditions of Replication Stress

The Sin3-histone deacetylase (HDAC) corepressor complex is conserved from yeast to humans. Mammals possess two highly related Sin3 proteins, mSin3A and mSin3B, which serve as scaffolds tethering HDAC...Full Text Available

2008-03-18

66

The histone chaperone Asf1 and the chromatin remodeler SWI/SNF have been separately implicated in derepression of the DNA damage response (DDR) genes in yeast cells treated with genotoxins that cause...Full Text Available

67

Taking Issue - Volume 4, Issue 6

The state of chromatin during the cell cycle was examined using synchronized cultures of CHO hamster cells. Results support Mazia's dynamic chromosome cycle model and indicate that DNA-interactive chemotherapeutic agents elicit different types of kinetic responses in treated cells, suggesting a degree of specificity of interaction between various alkylating and intercalating agents and the genome. Effects of sarkosyl crystals, heparin, and chemotherapeutic agents, neocarzinostatin and adriamycin, on chromation are discussed. (HLW)

1976-01-01

68

Shuttle Data Center File-Processing Tool in Java

governance through land use planning and mechanisms to deal with informal urban settlements in a manner that will facilitate improvements in

69

Process for retorting oil shale and the like

A Java-language computer program has been written to facilitate mining of data in files in the

2006-01-01

70

Motivation in vigilance - A test of the goal-setting hypothesis of the effectiveness of knowledge of results.

The production of oil by retorting shale and other hydrocarbonaceous and lignocellulosic solid materials is facilitated by retorting in the presence of steam and acetic acid.

1983-08-02

71

Long non-coding RNAs in nervous system function and disease

This study tested the prediction, derived from the goal-setting hypothesis, that the facilitating

1973-01-01

72

British Library Electronic Table of Contents (United Kingdom)

Central nervous system (CNS) development, homeostasis, stress responses, and plasticity are all mediated by epigenetic mechanisms that modulate gene expression and promote selective deployment of functional gene networks in response to complex profiles of interoceptive and environmental signals. Thus, not surprisingly, disruptions of these epigenetic processes are implicated in the pathogenesis of a spectrum of neurological and psychiatric diseases. Epigenetic mechanisms involve chromatin remodeling by relatively generic complexes that catalyze DNA methylation and various types of histone modifications. There is increasing evidence that these complexes are directed to their sites of action by long non-protein-coding RNAs (lncRNAs), of which there are tens if not hundreds of thousands speci...

2010-01-01

73

Local chromatin structure of heterochromatin regulates repeatedDNA stability, nucleolus structure, and genome integrity

Convoluted cells as a marker for maternal cell contamination in CVS cultures

Heterochromatin constitutes a significant portion of the genome in higher eukaryotes; approximately 30% in Drosophila and human. Heterochromatin contains a high repeat DNA content and a low density of protein-encoding genes. In contrast, euchromatin is composed mostly of unique sequences and contains the majority of single-copy genes. Genetic and cytological studies demonstrated that heterochromatin exhibits regulatory roles in chromosome organization, centromere function and telomere protection. As an epigenetically regulated structure, heterochromatin formation is not defined by any DNA sequence consensus. Heterochromatin is characterized by its association with nucleosomes containing methylated-lysine 9 of histone H3 (H3K9me), heterochromatin protein 1 (HP1) that binds H3K9me, and Su(var)3-9, which methylates H3K9 and binds HP1. Heterochromatin formation and functions are influenced by HP1, Su(var)3-9, and the RNA interference (RNAi) pathway. My thesis project investigates how ...

2007-05-05

74

DEFF Research Database (Denmark)

In order to identify cells of maternal origin in CVS cultures, tissue from 1st trimester abortions were cultivated and the cultures stained in situ for X-chromatin. Convoluted cells and maternal fibroblasts were found to be positive. By chromosome analysis of cultures from 105 diagnostic placenta biopsies, obtained by the transabdominal route, metaphases of maternal origin were found in nine cases. In eight of these cases colonies of convoluted cells were observed. We conclude that convoluted cells are of maternal origin and are a reliable marker for maternal cell contamination in CVS cultures.

1987-01-01

75

takeout, a Novel Drosophila Gene under Circadian Clock Transcriptional Regulation

View Transcript (111 Kb PDF) - NASA

We report the identification and characterization of a new Drosophila clock-regulated gene, takeout (to). to is a member of a novel...Full Text Available

2000-09-01

76

Transcriptional Regulation of Membrane Lipid Homeostasis in Escherichia coli*

Sep 8, 2005 ... one of the headquarters buildings here at Stennis, took off .... Also, they have a joint task force, that the ...

77

Stochastic gene expression and its consequences

The biophysical properties of membrane phospholipids are controlled by the composition of their constituent fatty acids and are tightly regulated in Escherichia...Full Text Available

2009-12-11

78

Induced Expression of Doublesex-and mab-3-related Transcription Factor-1 (DMRT-1) mRNA by Testosterone in the Olive Flounder, Paralichthys olivaceus ovary

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

79

KoreaScience (Korea)

Full Text Available

2007-08-01

80

INTERVIEW TRANSCRIPT - Johnson Space Center - NASA

Functional domain analysis of glass, a zinc-finger-containing transcription factor in Drosophila.

Nov 13, 2000 ... built to support the Milstar Program. That was pretty interesting, and I'll tell you, that was about the easiest job I ever had. ...

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Control of Host Cell Phosphorylation by Legionella Pneumophila

The glass gene is required for proper photo-receptor differentiation during development of the Drosophila eye glass codes for a DNA-binding protein containing five zinc fingers that we show is a transcriptional activator. A comparison of the sequences of the glass genes from two species of Drosophila and a detailed functional domain analysis of the Drosophila melanogaster glass gene reveal that both the DNA-binding domain and the transcriptional-activation domain are highly conserved between the two species. Analysis of the DNA-binding domain of glass indicates that the three carboxyl-terminal zinc fingers alone are necessary and sufficient for DNA binding. We also show that a deletion mutant of glass containing only the DNA-binding domain can behave in a dominant-negative manner both in vivo and in a cell culture assay that measures transcriptional activation. PMID:7604032

1995-07-01

82

Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter.

Phosphorylation is one of the most frequent modifications in intracellular signaling and is implicated in many processes ranging from transcriptional control to signal transduction in innate immunity....Full Text Available

83

Transcription from the SV40 early-early and late-early overlapping promoters in the absence of DNA replication.

The cell cycle-regulatory transcription factor E2F-1 is regulated by interactions with proteins such as the retinoblastoma gene product and by cell cycle-dependent alterations in E2F-1 mRNA abundance....Full Text Available

1994-10-01

84

Termination of transcription by bacteriophage T3 RNA polymerase: homogeneous 3'-terminal oligonucleotide sequence of in vitro T3 RNA polymerase transcripts.

Transcription for a hybrid SV40 promoter-beta globin coding sequence recombinant initiates from both early-early (EE) and late-early (LE) SV40 start sites (EES and LES) in the absence of DNA replication....Full Text Available

1983-01-01

85

Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA.

RNA was synthesized in vitro from a T3 DNA template by T3 RNA polymerase and subsequently separated into seven discrete size classes (molecular weights ranging between 0.21 x 10(6) and 6.2 x 10(6))...Full Text Available

1979-10-01

86

Comparative transcriptional pathway bioinformatic analysis of dietary restriction, Sir2, p53 and resveratrol life span extension in Drosophila

The nucleotide sequence of the 5'-terminal oligonucleotides produced by pancreatic RNase digestion of bacteriophage T3 RNA polymerase (EC 2.7.7.6) transcripts of T3 DNA has been determined. The sequence...Full Text Available

1980-07-01

87

Arginine aminoacylation identity is context-dependent and ensured by alternate recognition sets in the anticodon loop of accepting tRNA transcripts.

A multiple comparison approach using whole genome transcriptional arrays was used to identify genes and pathways involved in calorie restriction/dietary restriction (DR) life span extension in Drosophila....Full Text Available

2011-03-15

88

Alternative mRNA Splicing Produces a Novel Biologically Active Short Isoform of PGC-1?*

Yeast arginyl-tRNA synthetase recognizes the non-modified wild-type transcripts derived from both yeast tRNA(Arg) and tRNA(Asp) with equal efficiency. It discriminates its cognate natural substrate,...Full Text Available

1996-09-16

89

A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism.

The transcriptional co-activator PGC-1α regulates functional plasticity in adipose tissue by linking sympathetic input to the transcriptional program of adaptive thermogenesis. We report here...Full Text Available

2009-11-20

90

?₁-Fetoprotein Transcription Factor (FTF)/Liver Receptor Homolog-1 (LRH-1) Is an Essential Lipogenic Regulator

Transcription activation of sulfur metabolism in yeast is dependent on two DNA binding factors, the centromere binding factor 1 (Cbf1) and Met4. While the role of Met4 was clearly established by showing...Full Text Available

1996-05-15

91

Light-dependent regulation of DEL1 is determined by the antagonistic action of E2Fb and E2Fc.

α₁-Fetoprotein transcription factor (FTF), also known as liver receptor homolog 1 (LRH-1) is highly expressed in liver and intestine, where it is implicated in the regulation...Full Text Available

2010-04-01

92

Urine alkalization facilitates uric acid excretion

Endoreduplication represents a cell cycle variant during which multiple rounds of DNA replication occur without subsequent chromosome separation and cytokinesis, resulting into a cellular increase of the DNA content. Although the DNA ploidy level of cells is controlled by external stimuli such as light, currently limited knowledge is available on how environmental signals regulate the endoreduplication cycle at the molecular level. Previously, we have demonstrated that the conversion from the mitotic cell cycle into an endoreduplication cycle is mediated by the atypical E2F transcription factor DEL1 that operates as a repressor of endocycle onset. Here, we identified DEL1 as a transcriptional target of the classical E2Fb and E2Fc transcription factors that antagonistically control DEL1 transcript levels through competition for a single E2F cis-acting binding site. Correspondingly with the reported ...

2011-09-01

93

Uncoupler-resistant mutants of bacteria.

BackgroundIncrease in the incidence of hyperuricemia associated with gout as well as hypertension, renal diseases and cardiovascular diseases has been a public health concern. We...Full Text Available

94

The structure of receptor-associated protein (RAP)

The chemiosmotic model of energy transduction offers a satisfying and widely confirmed understanding of the action of uncouplers on such processes as oxidative phosphorylation; the uncoupler, by facilitating...Full Text Available

1990-03-01

95

Salivary cotinine concentrations in daily smokers in Barcelona, Spain: a cross-sectional study

The receptor-associated protein (RAP) is a molecular chaperone that binds tightly to certain newly synthesized LDL receptor family members in the endoplasmic reticulum (ER) and facilitates their delivery...Full Text Available

2007-08-01

96

Recent Reviews in Carcinogenesis 1981.

BackgroundCharacterizing and comparing the determinant of cotinine concentrations in different populations should facilitate a better understanding of smoking patterns and addiction....Full Text Available

97

Nail-Gun Injuries to the Hand

Recent Reviews in Carcinogenesis is a service of the International Cancer Research Data Bank (ICRDB) Program of the National Cancer Institute, intended to facilitate and promote the exchange of information between cancer scientists. It contains abstracts ...

1982-01-01

98

Korea Joins International Computing Infrastructure

Background: The nail gun is a commonly utilized tool in carpentry and construction. When used properly with appropriate safety precautions, it can facilitate production and boost efficiency;...Full Text Available

99

Differential facilitative and competitive effects of a dominant macrophyte in grazed subtropical wetlands

... Society Physics Press Release 05-175Korea Joins International Computing Infrastructure ... connectivity with Korea in a ceremony last month. Facilitated by the Korea Institute for Science and ...

100

British Library Electronic Table of Contents (United Kingdom)

Summary 1.-Plant-plant interactions fluctuate between competition and facilitation depending upon ecological conditions and species traits. Facilitative interactions are expected to increase in frequency via associational defences with increasing consumer pressure. The ability of species to cope with competition and/or ecological stressors may alter the outcome of plant-plant interactions. 2.-We conducted a transplant experiment to determine if native and non-native grasses and forbs respond similarly to interactions with Juncus effusus L., an unpalatable benefactor species, along a grazing intensity gradient in two contrasting pasture types: intensively managed and semi-natural. We expected competitive taller, erect species (grasses) and non-natives to obtain stronger facilitative effects...

2011-01-01

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101

Comparative genomics of insect juvenile hormone biosynthesis?

Effect of mutations in HNF-1#alpha# and HNF-1#beta# on the transcriptional regulation of human sucrase-isomaltase in Caco-2 cells

The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular...Full Text Available

2006-04-01

102

Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomography

Mutations in transcription factors hepatocyte nuclear factors (HNF)-1#alpha# and HNF-1#beta# cause maturity-onset diabetes of the young (MODY) types 3 and 5, respectively. HNF-1#alpha# and HNF-1#beta# mutations are well studied in some tissues, but the mechanism by which HNF-1#alpha# and HNF-1#beta# mutations affect sucrase-isomaltase (SI) transcription in the small intestine is unclear. We studied the effects of 13 HNF-1#alpha# mutants and 2 HNF-1#beta# mutants on human SI gene transcription, which were identified in subjects with MODY3 and MODY5, respectively. Transactivation activity of 11 HNF-1#alpha# and 2 HNF-1#beta# mutants was significantly lower than that of wild (wt)-HNF-1#alpha# and wt-HNF-1#beta#. Furthermore, in co-expression studies with mutant (mu)-HNF-1#alpha#/ wt-HNF-1#beta# and wt-HNF-1#alpha#/mu-HNF-1#beta#, the combination of mu-HNF-1#alpha# (P379fsdelCT and T539fsdelC)/wt-HNF-1#beta# impaired SI ...

2004-12-03

103

The Notch ligand Delta-like 1 integrates inputs from TGFbeta/Activin and Wnt pathways

We present a novel preparation method for studies of in vitro reconstituted mitotic chromosomes from Xenopus laevis egg extracts. This method involves a gentle adsorption of chromosomes from the extracts using surface affinity enrichment, followed by plunge freezing, freeze-substitution and cryo-embedding before examination by EM tomography. For comparison, chromosomes were also prepared by a conventional method, which included immobilization of chromosomes in agarose and a room-temperature dehydration (embedding) protocol. Three-dimensional reconstructions showed that samples prepared with the new method have a greater interconnectivity of sub-structural features and a higher compaction ratio together with an apparently less perturbed chromatin structure than those prepared using the conventional approach. The implications of the new method for the preparation of other difficult samples and additional application possibilities are discussed.

2005-07-15

104

British Library Electronic Table of Contents (United Kingdom)

Unlike the well-characterized nuclear function of the Notch intracellular domain, it has been difficult to identify a nuclear role for the ligands of Notch. Here we provide evidence for the nuclear function of the Notch ligand Delta-like 1 in colon cancer (CC) cells exposed to butyrate. We demonstrate that the intracellular domain of Delta-like 1 (Dll1icd) augments the activity of Wnt signaling-dependent reporters and that of the promoter of the connective tissue growth factor (CTGF) gene. Data suggest that Dll1icd upregulates CTGF promoter activity through both direct and indirect mechanisms. The direct mechanism is supported by co-immunoprecipitation of endogenous Smad2/3 proteins and Dll1 and by chromatin immunoprecipitation analyses that revealed the occupancy of Dll1icd on CTGF promot...

2011-01-01

105

Resveratrol causes COX-2- and p53-dependent apoptosis in head and neck squamous cell cancer cells

British Library Electronic Table of Contents (United Kingdom)

Cyclooxygenase-2 (COX-2) content is increased in many types of tumor cells. We have investigated the mechanism by which resveratrol, a stilbene that is pro-apoptotic in many tumor cell lines, causes apoptosis in human head and neck squamous cell carcinoma UMSCC-22B cells by a mechanism involving cellular COX-2. UMSCC-22B cells treated with resveratrol for 24 h, with or without selected inhibitors, were examined: (1) for the presence of nuclear activated ERK1/2, p53 and COX-2, (2) for evidence of apoptosis, and (3) by chromatin immunoprecipitation to demonstrate p53 binding to the p21 promoter. Stilbene-induced apoptosis was concentration-dependent, and associated with ERK1/2 activation, serine-15 p53 phosphorylation and nuclear accumulation of these proteins. These effects were blocked by ...

2008-01-01

106

Ionizing radiation is a potent inducer of mitotic recombination in mouse embryonic stem cells

British Library Electronic Table of Contents (United Kingdom)

Maintenance of genomic integrity in embryonic cells is pivotal to proper embryogenesis, organogenesis and to the continuity of species. Cultured mouse embryonic stem cells (mESCs), a model for early embryonic cells, differ from cultured somatic cells in their capacity to remodel chromatin, in their repertoire of DNA repair enzymes, and in the regulation of cell cycle checkpoints. Using 129XC3HF1 mESCs heterozygous for Aprt, we characterized loss of Aprt heterozygosity after exposure to ionizing radiation. We report here that the frequency of loss of heterozygosity mutants in mESCs can be induced several hundred-fold by exposure to 5-10Gy of X-rays. This induction is 50-100-fold higher than the induction reported for mouse adult or embryonic fibroblasts. The primary mechanism underlying the...

2011-01-01

107

Histone deacetylase inhibitors and transforming growth factor-b induce 15-hydroxyprostaglandin dehydrogenase expression in human lung adenocarcinoma cells

British Library Electronic Table of Contents (United Kingdom)

Histone deacetylase (HDAC) inhibitors have been actively exploited as potential anticancer agents. To identify gene targets of HDAC inhibitors, we found that HDAC inhibitors such as sodium butyrate, scriptaid, apicidin and oxamflatin induced the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), a potential cyclooxygenase-2 (COX-2) antagonist and tumor suppressor, in a time and concentration dependent manner in A549 and H1435 lung adenocarcinoma cells. Detailed analyses indicated that HDAC inhibitors activated the 15-PGDH promoter-luciferase reporter construct in transfected A549 cells. A representative HDAC inhibitor, scriptaid, and its negative structural analog control, nullscript, were further evaluated at the chromatin level. Scriptaid but not nullscript induced a signific...

2006-01-01

108

Preliminary crystallographic analysis of a possible transcription factor encoded by the mimivirus L544 gene

British Library Electronic Table of Contents (United Kingdom)

Mimivirus is the prototype of a new family (the Mimiviridae) of nucleocytoplasmic large DNA viruses (NCLDVs), which already include the Poxviridae, Iridoviridae, Phycodnaviridae and Asfarviridae. Mimivirus specifically replicates in cells from the genus Acanthamoeba. Proteomic analysis of purified mimivirus particles revealed the presence of many subunits of the DNA-directed RNA polymerase II complex. A fully functional pre-transcriptional complex appears to be loaded in the virions, allowing mimivirus to initiate transcription within the host cytoplasm immediately upon infection independently of the host nuclear apparatus. To fully understand this process, a systematic study of mimivirus proteins that are predicted (by bioinformatics) or suspected (by proteomic analysis) to be involved in...

2011-01-01

109

Effect of an Ostertagia ostertagi infection on the transcriptional stability of housekeeping genes in the bovine abomasum

British Library Electronic Table of Contents (United Kingdom)

Quantitative Real-Time PCR (qRT-PCR) is a widely used tool to study host responses against parasites. A crucial step in the gene quantification process is the normalization of the expression data against stable housekeeping genes (HKGs). However, in recent years, several reports have showed that the transcriptional levels of such HKGs can change dramatically, especially when cellular changes appear in the tissues investigated. The aim of the current study was to assess the variability of 11 putative HKGs in bovine abomasal tissue during an infection with the parasitic nematode Ostertagia ostertagi. Gene transcription levels of selected potential HKGs were measured by qRT-PCR and the expression stabilities evaluated using geNorm, NormFinder, and The Mann-Whitney-U test. The analysis showed ...

2011-01-01

110

Cell culture and gene transcription effects of copper sulfate on Chinese hamster ovary cells

British Library Electronic Table of Contents (United Kingdom)

Abstract This study reports the effects of varying concentrations of copper sulfate on the metabolic and gene transcriptional profile of a recombinant Chinese hamster ovary (CHO) cell line producing an immunoglobulin G (IgG)-fusion protein (B0). Addition of 50 M copper sulfate significantly decreased lactate accumulation in the cultures while increasing viable cell density and protein titer. These changes could be seen from day 6 and became increasingly evident with culture duration. Reducing the copper sulfate concentration to 5 M retained all the above beneficial effects, but with the added benefit of reduced levels of the aggregated form of the B0 protein. To profile the cellular changes due to copper sulfate addition at the transcriptional level, Affymetrix CHO microarrays were used to...

2011-01-01

111

var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2

Unbiased Transcriptional Comparisons of Generalist and Specialist Herbivores Feeding on Progressively Defenseless Nicotiana attenuata Plants

BackgroundThe pathogenicity of Plasmodium falciparum is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell...Full Text Available

112

Two separate domains within vesicular stomatitis virus phosphoprotein support transcription when added in trans.

BackgroundHerbivore feeding elicits dramatic increases in defenses, most of which require jasmonate (JA) signaling, and against which specialist herbivores are thought to be better...Full Text Available

113

Two Proline-Rich Nuclear Localization Signals in the Amino- and Carboxyl-Terminal Regions of the Borna Disease Virus Phosphoprotein

The structural phosphoprotein NS of vesicular stomatitis virus, in association with the virion-associated RNA polymerase L protein, transcribes the genome ribonucleoprotein template in vitro. It contains...Full Text Available

1987-12-01

114

Twist-1 is a PPAR ?-inducible, negative feedback regulator of PGC-1 ? in brown fat metabolism

Borna disease virus (BDV) uses a unique strategy of replication and transcription which takes place in the nucleus, unlike other known, nonsegmented, negative-stranded RNA viruses of animal origin....Full Text Available

1998-12-01

115

Transdifferentiation of myoblasts by the adipogenic transcription factors PPAR gamma and C/EBP alpha.

SummaryBrown fat is specialized in energy expenditure, a process that is principally controlled by the transcriptional co-activator PGC-1α. Here we describe a molecular...Full Text Available

2009-04-03

116

Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1

Skeletal muscle and adipose tissue development often has a reciprocal relationship in vivo, particularly in myodystrophic states. We have investigated whether determined myoblasts with no inherent adipogenic...Full Text Available

1995-10-10

117

Transcriptional mapping of the 3' end of the bovine syncytial virus genome.

Known vertebrate GATA proteins contain two zinc fingers and are required in development, whereas invertebrates express a class of essential proteins containing one GATA-type zinc finger. We isolated...Full Text Available

2001-04-02

118

Transcriptional coactivator PGC-1? promotes peroxisomal remodeling and biogenesis

The bovine syncytial virus, a member of the retroviral subfamily Spumavirinae, causes a persistent, asymptomatic infection in cattle. Nucleotide sequence analysis of the viral genome revealed two overlapping...Full Text Available

1994-02-01

119

Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1

Mitochondria and peroxisomes execute some analogous, nonredundant functions including fatty acid oxidation and detoxification of reactive oxygen species, and, in response to select metabolic cues, undergo...Full Text Available

2010-11-23

120

Tissue Effect on Genetic Control of Transcript Isoform Variation

Earlier we have shown that exogenous expression of HIPPI, a molecular partner of Huntingtin interacting protein HIP-1, induces apoptosis and increases expression of caspases-1, -8 and -10 in HeLa and...Full Text Available

2010-01-01

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121

The role of the antioxidant and longevity-promoting Nrf2 pathway in metabolic regulation

Current genome-wide association studies (GWAS) are moving towards the use of large cohorts of primary cell lines to study a disease of interest and to assign biological relevance to the genetic signals...Full Text Available

2009-08-01

122

The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription

Purpose of ReviewThe vertebrate cap’n’collar family transcription factor Nrf2 and its invertebrate homologs SKN-1 (in worms) and CncC (in flies) function...Full Text Available

2011-01-01

123

The protozoan parasite Theileria annulata alters the differentiation state of the infected macrophage and suppresses musculoaponeurotic fibrosarcoma oncogene (MAF) transcription factors

The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex...Full Text Available

2005-01-01

124

The crystal structure of unmodified tRNA^Phe from Escherichia coli

AbstractThe tick-borne protozoan parasite Theileria annulata causes a debilitating disease of cattle called Tropical Theileriosis. The parasite predominantly invades...Full Text Available

2009-08-01

125

The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.

Post-transcriptional nucleoside modifications fine-tune the biophysical and biochemical properties of transfer RNA (tRNA) so that it is optimized for participation in cellular processes. Here we report...Full Text Available

2010-07-01

126

The Specificity of Innate Immune Responses Is Enforced by Repression of Interferon Response Elements by NF-?B p50

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection...Full Text Available

1983-10-25

127

The Regulation of Aging and Longevity

The specific binding of transcription factors to cognate sequence elements is thought to be critical for the generation of specific gene expression programs. Members of the nuclear factor κB...Full Text Available

128

The Induction of APC with a Distinct Tolerogenic Phenotype via Contact-Dependent STAT3 Activation

p53 plays a critical role in tumor suppression. As a transcription factor, in response to stress signals, p53 regulates its target genes and initiates stress responses, including cell cycle arrest,...Full Text Available

2011-04-01

129

The Anti-Inflammatory Drug Leflunomide Is an Agonist of the Aryl Hydrocarbon Receptor

BackgroundActivation of the signal transducer and activator of transcription 3 (STAT3) within antigen presenting cells (APCs) is linked to abnormal APCs differentiation and function....Full Text Available

130

Synergistic Operation of the CAR2 (Ornithine Transaminase) Promoter Elements in Saccharomyces cerevisiae

BackgroundThe aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the toxicity and biological activity of dioxins and related chemicals. The...Full Text Available

131

Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica

Dal82p binds to the UIS_ALL sites of allophanate-induced genes of the allantoin-degradative pathway and functions synergistically with the GATA family Gln3p and Gat1p transcriptional...Full Text Available

1999-11-01

132

Structure of the Taz2 domain of p300: insights into ligand binding

BackgroundThe common liver fluke Fasciola hepatica is the agent of a zoonosis with significant economic consequences in livestock production worldwide, and increasing...Full Text Available

133

Structural and Functional Insights into Saccharomyces cerevisiae Tpa1, a Putative Prolylhydroxylase Influencing Translation Termination and Transcription*

CBP and its paralog p300 are histone acetyl transferases that regulate gene expression by interacting with multiple transcription factors via specialized domains. The structure...Full Text Available

2009-12-01

134

Strand displacement synthesis capability of Moloney murine leukemia virus reverse transcriptase.

Efficiency of translation termination relies on the specific recognition of the three stop codons by the eukaryotic translation termination factor eRF1. To date only a few proteins are known to be involved...Full Text Available

2010-10-01

135

Short Stat5-Interacting Peptide Derived from Phospholipase C-?3 Inhibits Hematopoietic Cell Proliferation and Myeloid Differentiation

The accepted model of retroviral reverse transcription includes a circular DNA intermediate which requires strand displacement synthesis for linearization and creation of an integration-competent, long...Full Text Available

1994-08-01

136

Shielding of Sleeping Beauty DNA Transposon-delivered Transgene Cassettes by Heterologous Insulators in Early Embryonal Cells

Constitutive activation of the transcription factor Stat5 in hematopoietic stem/progenitor cells leads to various hematopoietic malignancies including myeloproliferative neoplasm (MPN). Our recent study...Full Text Available

137

Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts

The Sleeping Beauty (SB) transposon system represents an important alternative to viral integrating vector systems but may, as its viral counterparts, be subject to transcriptional silencing. To investigate...Full Text Available

2009-01-01

138

Sequence features that drive human promoter function and tissue specificity

Although genetically identical for autosomal Chrs (Chr), male and female preimplantation embryos could display sex-specific transcriptional regulation. To illustrate sex-specific differences at the...Full Text Available

2010-02-23

139

Selective Degradation of Newly Synthesized Nonmessenger Simian Virus 40 Transcripts

Promoters are important regulatory elements that contain the necessary sequence features for cells to initiate transcription. To functionally characterize a large set of human promoters, we measured...Full Text Available

2010-07-01

140

Scintillation proximity assay for measurement of RNA methylation

By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [³H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have...Full Text Available

1978-11-01

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141

Role of histone methylation and demethylation in adipogenesis and obesity

Methylation of RNA by methyltransferases is a phylogenetically ubiquitous post-transcriptional modification that occurs most extensively in transfer RNA (tRNA) and ribosomal RNA (rRNA). Biochemical...Full Text Available

2009-03-01

142

Retinoic acid X receptor in the diploblast, Tripedalia cystophora

Adipocyte differentiation is a complex developmental process that involves the coordinated interplay of numerous transcription factors. PPARγ has emerged as a master regulator of adipogenesis...Full Text Available

2010-01-01

143

Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex

Nuclear hormone receptors comprise a characteristic family of transcription factors found in vertebrates, insects and nematodes. Here we show by cDNA and gene cloning that a Cnidarian, Tripedalia...Full Text Available

1998-11-10

144

Regulation of apoptosis by the circadian clock through NF-?B signaling

Brown fat is a specialized tissue that can dissipate energy and counteract obesity through a pattern of gene expression that greatly increases mitochondrial content and uncoupled respiration. PRDM16...Full Text Available

2008-05-15

145

Reciprocal Silencing, Transcriptional Bias and Functional Divergence of Homeologs in Polyploid Cotton (Gossypium)

In mice and humans the circadian rhythm of many biochemical reactions, physiology, and behavior is generated by a transcriptional-translation feedback loop (TTFL) made up of the so-called core clock...Full Text Available

2011-07-19

146

Prediction and diagnosis of bladder cancer recurrence based on urinary content of hTERT, SENP1, PPP1CA, and MCM5 transcripts

Polyploidy is an important force in the evolution of flowering plants. Genomic merger and doubling induce an extensive array of genomic effects, including immediate and long-term alterations in the...Full Text Available

2009-06-01

147

Precise temporal control of the eye regulatory gene Pax6 via enhancer-binding site affinity

BackgroundIdentification of urinary biomarkers for detection of bladder cancer recurrence would be beneficial to minimize the frequency of cystoscopy. Our objective was to determine...Full Text Available

148

Osterix Overexpression in Mesenchymal Stem cells Stimulates Healing of Critical-Sized Defects in Murine Calvarial Bone

How transcription factors interpret the cis-regulatory logic encoded within enhancers to mediate quantitative changes in spatiotemporally restricted expression patterns during animal...Full Text Available

2010-05-15

149

Nuclear-Cytoplasmic Shuttling of Menin Regulates Nuclear Translocation of ?-Catenin?

Osterix (Osx) is a zinc-finger-containing transcription factor that is expressed in osteoblasts of all endochondral and membranous bones. In Osx null ...Full Text Available

2007-10-01

150

NKX2-3 Transcriptional Regulation of Endothelin-1 and VEGF Signaling in Human Intestinal Microvascular Endothelial Cells

Menin, which is encoded by the multiple endocrine neoplasia type 1 (MEN1) gene, is a tumor suppressor and transcriptional regulator. Menin controls proliferation and apoptosis of cells, especially pancreatic...Full Text Available

2009-10-01

151

NF-?B and cancer: how intimate is this relationship

BackgroundNKX2-3 is associated with inflammatory bowel disease (IBD). NKX2-3 is expressed in microvascular endothelial cells and the muscularis...Full Text Available

152

Molecular dynamics of a ?B DNA element: base flipping via cross-strand intercalative stacking in a microsecond-scale simulation

NF-κB, a transcription factor first discovered in 1986, is now known to be closely connected to the process of tumorogenesis based on a multiplicity of evidence. (1)...Full Text Available

2010-03-01

153

Mapping of the transcription start site (TSS) and identification of SNPs in the bovine neuropeptide Y (NPY) gene

The sequence-dependent structural variability and conformational dynamics of DNA play pivotal roles in many biological milieus, such as in the site-specific binding of transcription factors to target...Full Text Available

2008-09-01

154

Malignant Catarrhal Fever of Cattle Is Associated with Low Abundance of IL-2 Transcript and a Predominantly Latent Profile of Ovine Herpesvirus 2 Gene Expression

BackgroundNeuropeptide Y is a key neurotransmitter of the central nervous system which plays a vital role in the feed energy homeostasis in mammals. Mutations in the regulatory and...Full Text Available

155

Kinetic Complexity of the Global Response to Glucocorticoid Receptor Action

BackgroundMalignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes...Full Text Available

156

Ixodes scapularis tick serine proteinase inhibitor (serpin) gene family; annotation and transcriptional analysis

We have characterized the kinetic response of gene targets throughout the murine genome to transcriptional modulation by the glucocorticoid receptor (GR). In contrast to a model in which multiple genes...Full Text Available

2009-04-01

157

Interleukin-7 mediates glucose utilization in lymphocytes through transcriptional regulation of the hexokinase II gene

BackgroundSerine proteinase inhibitors (Serpins) are a large superfamily of structurally related, but functionally diverse proteins that control essential proteolytic pathways in...Full Text Available

158

Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators

The cytokine interleukin-7 (IL-7) has essential growth activities that maintain the homeostatic balance of the immune system. Little is known of the mechanism by which IL-7 signaling regulates metabolic...Full Text Available

2010-06-01

159

Increased transcription of the c-myc oncogene in two methylcholanthrene-induced quail fibroblastic cell lines

Juvenile hormone analog (JHA) insecticides are relatively nontoxic to vertebrates and offer effective control of certain insect pests. Recent reports of resistance in whiteflies and mosquitoes demonstrate...Full Text Available

1998-03-17

160

Increased SRF transcriptional activity in human and mouse skeletal muscle is a signature of insulin resistance

The expression of three c-onc genes (c-erb, c-myc, c-myb) was investigated in five cell lines established from fibrosarcomas induced with 20-methylcholanthrene (MCA) of Japanese quails. These cell lines showed low levels of the three c-onc genes, with the exception of two cell lines that accumulated moderate (MCAQ 1-4) and large amounts (MCAQ 3-5) of c-myc RNA. Molecular cloning and restriction endonuclease analyses indicated that expression of c-myc in these two cell lines were not associated with detectable rearrangements in the c-myc locus, that the size of the c-myc transcript (2.7 kb) in MCAQ 3-5 was similar to that of the normal c-myc messenger RNAs (mRNA) and that the transcriptional activatin observed in MCAQ 3-5 was not mediated by the LTR (long terminal repeat) of a proximate ALV (avian leukosis virus) provirus. Finally, when analyzed with the restriction enzymes Msp I and Hpa II, the c-myc locus of MCAQ 3-5 and MCAQ 1-4 was found ...

1984-12-01

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161

Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies

Insulin resistance in skeletal muscle is a key phenotype associated with type 2 diabetes (T2D) for which the molecular mediators remain unclear. We therefore conducted an expression analysis of human...Full Text Available

2011-03-01

162

Identification of novel monosodium urate crystal regulated mRNAs by transcript profiling of dissected murine air pouch membranes

The spliced alignment of expressed sequence data to genomic sequence has proven a key tool in the comprehensive annotation of genes in eukaryotic genomes. A novel algorithm was developed to assemble...Full Text Available

2003-10-01

163

Hyperoxia enhances VEGF release from A549 cells via post-transcriptional processes

IntroductionThe murine air pouch is a bursa-like space that resembles the human synovial membrane. Injection of monosodium urate (MSU) crystals into the pouch elicits an acute inflammatory...Full Text Available

2008-01-01

164

Human mitochondrial transcription factor A reduction and mitochondrial dysfunction in Hashimoto's hypothyroid myopathy.

Exposure of animals to hyperoxia decreases lung VEGF mRNA expression concomitant with an acute increase in VEGF protein within the epithelial lining fluid (ELF). The VEGF concentration in ELF...Full Text Available

2007-09-01

165

Human intronic enhancers control distinct sub-domains of Gli3 expression during mouse CNS and limb development

BACKGROUND: Mitochondrial changes have been described in muscle tissue in acquired hypothyroidism. Among the molecular mechanisms by which thyroid hormones regulate expression of nuclear genes encoding...Full Text Available

2002-06-01

166

Human Pol II promoter recognition based on primary sequences and free energy of dinucleotides

BackgroundThe zinc-finger transcription factor GLI3 is an important mediator of Sonic hedgehog signaling and crucial for patterning of many aspects of the vertebrate body plan. In...Full Text Available

167

Human Immunodeficiency Virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells

BackgroundPromoter region plays an important role in determining where the transcription of a particular gene should be initiated. Computational prediction of eukaryotic Pol II promoter...Full Text Available

168

HPV16 E2 could act as down-regulator in cellular genes implicated in apoptosis, proliferation and cell differentiation

BackgroundHIV-1 reverse transcriptase (RT) is a heterodimer composed of p66 and p51 subunits and is responsible for reverse transcription of the viral RNA genome into DNA. RT can...Full Text Available

169

Global Analysis of the General Stress Response of Bacillus subtilis

BackgroundHuman Papillomavirus (HPV) E2 plays several important roles in the viral cycle, including the transcriptional regulation of the oncogenes E6 and E7, the regulation of the...Full Text Available

170

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Gene arrays containing all currently known open reading frames of Bacillus subtilis were used to examine the general stress response of Bacillus. By proteomics, transcriptional...Full Text Available

2001-10-01

171

Genome-Wide Transcriptional Response of Chemostat-Cultured Escherichia coli to Zinc

Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their in vivo counterparts in many aspects and exhibit a higher proportion of developmental...Full Text Available

2008-12-16

172

Genome-Wide Analysis Reveals a Major Role in Cell Fate Maintenance and an Unexpected Role in Endoreduplication for the Drosophila FoxA Gene Fork Head

Zinc is an essential trace metal ion for growth, but an excess of Zn is toxic and microorganisms express diverse resistance mechanisms. To understand global bacterial responses to excess Zn, we conducted...Full Text Available

2005-02-01

173

Evidence for Diversity in Transcriptional Profiles of Single Hematopoietic Stem Cells

Transcription factors drive organogenesis, from the initiation of cell fate decisions to the maintenance and implementation of these decisions. The Drosophila embryonic salivary gland...Full Text Available

174

Efficient discovery of ASCL1 regulatory sequences through transgene pooling

Hematopoietic stem cells replenish all the cells of the blood throughout the lifetime of an animal. Although thousands of stem cells reside in the bone marrow, only a few contribute to blood production...Full Text Available

2006-09-01

175

Effects of acute dieldrin exposure on neurotransmitters and global gene transcription in largemouth bass (Micropterus salmoides) hypothalamus

Zebrafish transgenesis is a powerful and increasingly common strategy to assay vertebrate transcriptional regulatory control. Several challenges remain, however, to the broader application of...Full Text Available

2010-06-01

176

Effects of ATRA combined with citrus and ginger-derived compounds in human SCC xenografts

Exposure to dieldrin induces neurotoxic effects in the vertebrate CNS and disrupts reproductive processes in teleost fish. Reproductive impairment observed in fish by dieldrin is likely the...Full Text Available

2010-08-01

177

Effect of pH and Temperature on Denitrification Gene Expression and Activity in Pseudomonas mandelii?

BackgroundNF-κB is a survival signaling transcription factor complex involved in the malignant phenotype of many cancers, including squamous cell carcinomas (SCC). The citrus...Full Text Available

178

Drosophila melanogaster Methoprene-tolerant (Met) gene homologs from three mosquito species: members of PAS transcriptional factor family

Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR....Full Text Available

2009-06-01

179

Downstream genes of Pax6 revealed by comprehensive transcriptome profiling in the developing rat hindbrain

The Methoprene-tolerant (Met) gene in Drosophila melanogaster has been shown to function in juvenile hormone (JH) action. Met...Full Text Available

2007-03-01

180

DosT and DevS are oxygen-switched kinases in Mycobacterium tuberculosis

BackgroundThe transcription factor Pax6 is essential for the development of the central nervous system and it exerts its multiple functions by regulating the expression of downstream...Full Text Available

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181

Djinn Lite: a tool for customised gene transcript modelling, annotation-data enrichment and exploration

Exposure of Mycobacterium tuberculosis to hypoxia is known to alter the expression of many genes, including ones thought to be involved in latency, via the transcription factor DevR...Full Text Available

2007-08-01

182

Does My Child Have ADHD?

Medline Plus

September 14, 2010 ADHD: Signs, Symptoms, Research NIMH researchers talk about the symptoms of ADHD as well as the latest research. Download this video. Watch on YouTube. Transcript Announcer: ADHD- Attention Deficit Hyperactivity Disorder- is one of the ...

183

Differential interleukin-6/Stat3 signaling as a function of cellular context mediates Ras-induced transformation

BackgroundThere is an ever increasing rate of data made available on genetic variation, transcriptomes and proteomes. Similarly, a growing variety of bioinformatic programs are becoming...Full Text Available

184

Differential Gene Expression in Primary Human Skin Keratinocytes and Fibroblasts in Response to Ionizing Radiation

IntroductionTyrosine phosphorylated signal transducer and activator of transcription 3 (pStat3) is expressed in numerous cancers and is required for mediating tumorigenesis. Autocrine...Full Text Available

2010-01-01

185

Development of a Selective Modulator of Aryl Hydrocarbon (Ah) Receptor Activity that Exhibits Anti-Inflammatory Properties

Although skin is usually exposed during human exposures to ionizing radiation, there have been no thorough examinations of the transcriptional response of skin fibroblasts and keratinocytes...Full Text Available

2009-07-01

186

Deficiency of circadian protein CLOCK reduces lifespan and increases age-related cataract development in mice

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin. However, the role of the AHR...Full Text Available

2010-05-17

187

Copy Number Variation and Transposable Elements Feature in Recent, Ongoing Adaptation at the Cyp6g1 Locus

Circadian clock is implicated in the regulation of aging. The transcription factor CLOCK, a core component of the circadian system, operates in complex with another circadian clock protein BMAL1. Recently...Full Text Available

188

Comparative transcripts profiling reveals new insight into molecular processes regulating lycopene accumulation in a sweet orange (Citrus sinensis) red-flesh mutant

The increased transcription of the Cyp6g1 gene of Drosophila melanogaster, and consequent resistance to insecticides such as DDT, is a widely cited example of adaptation...Full Text Available

2010-06-01

189

Cocaine induces cell death and activates the transcription nuclear factor kappa-b in pc12 cells

BackgroundInterest in lycopene metabolism and regulation is growing rapidly because accumulative studies have suggested an important role for lycopene in human health promotion....Full Text Available

190

Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis.

Cocaine is a worldwide used drug and its abuse is associated with physical, psychiatric and social problems. The mechanism by which cocaine causes neurological damage is very complex and involves several...Full Text Available

191

Chemical complementation: A reaction-independent genetic assay for enzyme catalysis

Cellular cholesterol homeostasis is controlled by sterol-regulated proteolysis of membrane-bound transcription factors called sterol-regulatory element binding proteins (SREBPs). CPP32, a cysteine protease,...Full Text Available

1996-03-01

192

Cbk1 regulation of the RNA binding protein Ssd1 integrates cell fate with translational control

A high-throughput assay for enzyme activity has been developed that is reaction independent. In this assay, a small-molecule yeast three-hybrid system is used to link enzyme catalysis to transcription...Full Text Available

2002-12-24

193

Bunyamwera virus can repair both insertions and deletions during RNA replication

SummarySpatial control of gene expression, at the level of both transcription and translation, is critical for cellular differentiation [1-Full Text Available

2009-12-29

194

Brg1 Is Required for Cdx2-Mediated Repression of Oct4 Expression in Mouse Blastocysts

The genomic termini of RNA viruses contain essential cis-acting signals for such diverse functions as packaging, genome translation, mRNA transcription, and RNA replication, and thus...Full Text Available

2010-06-01

195

Adrenaline modulates the global transcriptional profile of Salmonella revealing a role in the antimicrobial peptide and oxidative stress resistance responses

During blastocyst formation the segregation of the inner cell mass (ICM) and trophectoderm is governed by the mutually antagonistic effects of the transcription factors Oct4 and Cdx2. Evidence indicates...Full Text Available

196

Activated PPAR? Targets Surface and Intracellular Signals That Inhibit the Proliferation of Lung Carcinoma Cells

BackgroundThe successful interaction of bacterial pathogens with host tissues requires the sensing of specific chemical and physical cues. The human gut contains a huge number of...Full Text Available

197

Abnormalities of GATA-1 in Megakaryocytes from Patients with Idiopathic Myelofibrosis

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the nuclear hormone receptor superfamily. Their discovery in the 1990s provided insights...Full Text Available

2008-01-01

198

ATM-Dependent Phosphorylation of ATF2 Is Required for the DNA Damage Response

The abnormal megakaryocytopoiesis associated with idiopathic myelofibrosis (IM) plays a role in its pathogenesis. Because mice with defective expression of transcription factor GATA-1 (GATA-1^low...Full Text Available

2005-09-01

199

A small RNA promotes siderophore production through transcriptional and metabolic remodeling

SummaryActivating transcription factor 2 (ATF2) is regulated by JNK/p38 in response to stress. Here, we demonstrate that the protein kinase ATM phosphorylates ATF2 on serines...Full Text Available

2005-05-27

200

A highly conserved regulatory element controls hematopoietic expression of GATA-2 in zebrafish

Siderophores are essential factors for iron (Fe) acquisition in bacteria during colonization and infection of eukaryotic hosts, which restrain iron access through iron-binding protein, such as lactoferrin...Full Text Available

2010-08-24

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201

A global role for KLF1 in erythropoiesis revealed by ChIP-seq in primary erythroid cells

BackgroundGATA-2 is a transcription factor required for hematopoietic stem cell survival as well as for neuronal development in vertebrates. It has been shown that specific expression...Full Text Available

202

A classification-based framework for predicting and analyzing gene regulatory response

KLF1 regulates a diverse suite of genes to direct erythroid cell differentiation from bipotent progenitors. To determine the local cis-regulatory contexts and transcription factor networks...Full Text Available

2010-08-01

203

A Turquoise Mutant Genetically Separates Expression of Genes Encoding Phycoerythrin and Its Associated Linker Peptides

BackgroundWe have recently introduced a predictive framework for studying gene transcriptional regulation in simpler organisms using a novel supervised learning algorithm called...Full Text Available

204

A Response Regulator That Represses Transcription of Several Virulence Operons in the Group A Streptococcus

During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes...Full Text Available

2002-02-01

205

A Curated Database of miRNA Mediated Feed-Forward Loops Involving MYC as Master Regulator

A search for homologs of the Bacillus subtilis PhoP response regulator in the group A streptococcus (GAS) genome revealed three good candidates. Inactivation of one of these, recently...Full Text Available

1999-06-01

206

?Activation of the mitogen-activated protein kinase pathways by heat shock

BackgroundThe MYC transcription factors are known to be involved in the biology of many human cancer types. But little is known about the Myc/microRNAs cooperation in the regulation...Full Text Available

207

1 Transcript of Presidential Meeting in the ... - NASA History Office

In addition to inducing new transcriptional activities that lead within a few hours to the accumulation of heat shock proteins (Hsps), heat shock activates within minutes the major signaling transduction...Full Text Available

2002-04-01

208

Structure, Function, and Evolution of Rice Centromeres

increased knowledge as result of about a year's work, has led us first to ...... James Webb: And the advanced technology on which military power is going to be ..... on the road and I've been with the real big dogs and I know the deal ...

209

Nutrition and Physical Activity in Aging, Obesity, and Cancer

The centromere is the most characteristic landmark of eukaryotic chromosomes. Centromeres function as the site for kinetochore assembly and spindle attachment, allowing for the faithful pairing and segregation of sister chromatids during cell division. Characterization of centromeric DNA is not only essential to understand the structure and organization of plant genomes, but it is also a critical step in the development of plant artificial chromosomes. The centromeres of most model eukaryotic species, consist predominantly of long arrays of satellite DNA. Determining the precise DNA boundary of a centromere has proven to be a difficult task in multicellular eukaryotes. We have successfully cloned and sequenced the centromere of rice chromosome 8 (Cen8), representing the first fully sequenced centromere from any multicellular eukaryotes. The functional core of Cen8 spans ~800 kb of DNA, which was determined by chromatin immunoprecipitation (ChIP) using an antibody ...

2010-02-04

210

British Library Electronic Table of Contents (United Kingdom)

The liver is a primary target of growth hormone (GH). GH signals are mediated by the transcription factor signal transducer and activator of transcription 5 (STAT5). Here, we focus on recent discoveries about the role of GH-STAT5 signaling in hepatic physiology and pathophysiology. We discuss roles of the GH-STAT5 axis in body growth, lipid metabolism, and the cell cycle pertaining to hepatosteatosis, fibrosis, and hepatocellular carcinoma. Finally, we discuss recent discoveries about the role of GH-STAT5 in sex-specific gene expression and bile acid, steroid, and drug metabolism.

2011-01-01

211

The C'-terminal interaction domain of the thyroid hormone receptor confers the ability of the DNA site to dictate positive or negative transcriptional activity

Facilitated Proton Transfer by a Novel 2-Aminothiazole Derivative Across the Water/1,2-Dichloroethane Interface

To investigate mechanisms responsible for positive and negative transcriptional control, the authors have utilized two types of promoters that are diffferentially regulated by thyroid hormone (T{sub 3}) receptors. Promoters containing the palindromic T{sub 3} response element TCAGGTCA TGACCTGA are positively regulated by the T{sub 3} receptor after the administration of T{sub 3}, whereas otherwise identical promoters containing the estrogen response element TCAGGTCA CTG TGACCTGA can be regulated negatively; converse effects are observed with the estrogen receptor. They describe evidence that the transcriptional inhibitory effects of the T{sub 3} or estrogen receptors on the estrogen or T{sub 3} response elements, respectively, are imposed by amino acid sequences in the C'-terminal region that colocalize with dimerization and hormone-binding domains and that these sequences can transfer inhibitory functions to other classes of ...

1990-10-01

212

British Library Electronic Table of Contents (United Kingdom)

The behavior of proton transfer facilitated by a novel thiazole derivative, N-methyl-4-(4-phenoxyphenyl)thiazol-2-amine (MPPT), across the water/1,2-dichloroethane (1,2-DCE) interface was investigated electrochemically. The ionic partition diagram for MPPT was obtained from interpretation of the cyclic voltammograms. The apparent partition coefficient of MPPT was evaluated by the shaking-flask method under experimental conditions, while that for the protonated form of MPPT was calculated from its transfer potential obtained from the ionic partition diagram. It was suggested that the mechanism for transfer of MPPT across the water/1,2-DCE) interface depends on the pH of the aqueous phase. The parameters of the facilitated proton transfer across the water/1,2-DCE interface were evaluated as ...

2011-01-01

213

chipD: a web tool to design oligonucleotide probes for high-density tiling arrays

Using knowledge brokers to facilitate the uptake of pediatric measurement tools into clinical practice: a before-after intervention study

chipD is a web server that facilitates design of DNA oligonucleotide probes for high-density tiling arrays, which can be used in a number of genomic applications such as ChIP-chip or gene-expression...Full Text Available

2010-07-01

214

Translation Elongation Factor 1A Facilitates the Assembly of the Tombusvirus Replicase and Stimulates Minus-Strand Synthesis

BackgroundThe use of measurement tools is an essential part of good evidence-based practice; however, physiotherapists (PTs) are not always confident when selecting, administering,...Full Text Available

215

Top-down and bottom-up modulation in processing bimodal face/voice stimuli

Replication of plus-strand RNA viruses depends on host factors that are recruited into viral replicase complexes. Previous studies showed that eukaryotic translation elongation factor (eEF1A) is one...Full Text Available

2010-11-01

216

The human peripheral subunit-binding domain folds rapidly while overcoming repulsive Coulomb forces

BackgroundProcessing of multimodal information is a critical capacity of the human brain, with classic studies showing bimodal stimulation either facilitating or interfering in perceptual...Full Text Available

217

Surfactant protein A, an innate immune factor, is expressed in the vaginal mucosa and is present in vaginal lavage fluid

Peripheral subunit binding domains (PSBDs) are integral parts of large multienzyme complexes involved in carbohydrate metabolism. PSBDs facilitate shuttling of prosthetic groups between different catalytic...Full Text Available

2010-09-01

218

Steam turbines for cogeneration plants; Dampfturbinen fuer die Kraft-Waerme-Kopplung

Surfactant protein A (SP-A), first identified as a component of the lung surfactant system, is now recognized to be an important contributor to host defence mechanisms. SP-A can facilitate phagocytosis...Full Text Available

2004-01-01

219

Separation of the bacterial species, Escherichia coli, from mixed-species microbial communities for transcriptome analysis

The demand for cogeneration plants is growing worldwide. For the combination of electricity generation and heat extraction in power stations facilitates an excellent fuel exploitation and makes the operator of the plant independent from external energy suppliers. (orig.)

2006-07-01

220

Selective Enrichment of Azide-Containing Peptides from Complex Mixtures

BackgroundThe study of bacterial species interactions in a mixed-species community can be facilitated by transcriptome analysis of one species in the community using cDNA microarray...Full Text Available

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221

Rab protein evolution and the history of the eukaryotic endomembrane system

A general method is described to sequester peptides containing azides from complex peptide mixtures, aimed at facilitating mass spectrometric analysis to study different aspects of proteome...Full Text Available

2009-07-01

222

Prosocial effects of nicotine and ethanol in adolescent rats through partially dissociable neurobehavioral mechanisms

Spectacular increases in the quantity of sequence data genome have facilitated major advances in eukaryotic comparative genomics. By exploiting homology with classical model organisms, this makes possible...Full Text Available

2010-10-01

223

Near Infrared-Fluorescent and Magnetic Resonance Imaging Molecular Probe with High T1 Relaxivity for In Vivo Multimodal Imaging

The widespread use of tobacco and alcohol among adolescents might be related to the ability of nicotine and ethanol to facilitate social interactions. To investigate the neurobehavioral mechanisms...Full Text Available

2009-11-01

224

Metal Ions-Stimulated Iron Oxidation in Hydroxylases Facilitates Stabilization of HIF-1? Protein

A new gadolinium chelating NIR fluorescent molecular probe increases T₁ relaxivity of water protons, facilitating combined optical and magnetic resonance imaging.

2010-06-07

225

Medical language processing with SGML display.

The exposure of cells to several metal ions stabilizes HIF-1α protein. However, the molecular mechanisms are not completely understood. They may involve inhibition of hydroxylation by either...Full Text Available

2009-02-01

226

Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera

The paper demonstrates several ways that medical language processing can be combined with emerging display technologies to facilitate the extraction of data from free-text patient documents. The techniques...Full Text Available

1996-01-01

227

Impact of informed-choice invitations on diabetes screening knowledge, attitude and intentions: an analogue study

Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing...Full Text Available

2011-01-01

228

Image Engine: an object-oriented multimedia database for storing, retrieving and sharing medical images and text.

BackgroundDespite concerns that facilitating informed choice would decrease diabetes screening uptake, 'informed choice' invitations that increased knowledge did not affect attendance...Full Text Available

229

Exogenous Melatonin Administration Affects Self-grooming and Conspecific Odor Preferences in Long-Photoperiod Meadow Voles (Microtus pennsylvanicus)

This paper describes Image Engine, an object-oriented, microcomputer-based, multimedia database designed to facilitate the storage and retrieval of digitized biomedical still images, video, and text...Full Text Available

1993-01-01

230

Evaluation of Tumor Micro-Environment in an Animal Model using a Nanoparticle Contrast Agent in Computed Tomography Imaging

For meadow voles, Microtus pennsylvanicus, seasonal differences exist in self-grooming and in odor preferences for conspecifics, two behaviors which facilitate sexual interactions...Full Text Available

2007-06-08

231

Entropic effects in channel-facilitated transport: Inter-particle interactions break the flux symmetry

RATIONALE AND OBJECTIVESNon-invasive longitudinal imaging of tumor vasculature could provide new insights into the development of solid tumors, facilitating efficient...Full Text Available

2011-01-01

232

Dissolved Hydrocarbons and Related Microflora in a Fjordal Seaport: Sources, Sinks, Concentrations, and Kinetics

We analyze transport through conical channels due to the difference in particle concentration on the two sides of the membrane. Because of the detailed balance, fluxes of non-interacting particles...Full Text Available

2009-08-01

233

Design of 240,000 orthogonal 25mer DNA barcode probes

The continuous addition of toluene as a solute of treated ballast water from oil tankers into a well-defined estuary facilitated the study of the dynamics of dissolved hydrocarbon metabolism in seawater....Full Text Available

1981-10-01

234

Defining the Specificity of Cotranslationally Acting Chaperones by Systematic Analysis of mRNAs Associated with Ribosome-Nascent Chain Complexes

DNA barcodes linked to genetic features greatly facilitate screening these features in pooled formats using microarray hybridization, and new tools are needed to design large sets of barcodes to allow...Full Text Available

2009-02-17

235

Clinical Digital Libraries Project: design approach and exploratory assessment of timely use in clinical environments*

Polypeptides exiting the ribosome must fold and assemble in the crowded environment of the cell. Chaperones and other protein homeostasis factors interact with newly translated polypeptides to facilitate...Full Text Available

2011-07-01

236

Clarifying the Factors that Undermine Behavioral Inhibition System Functioning in Psychopathy

Objective: The paper describes and evaluates the use of Clinical Digital Libraries Project (CDLP) digital library collections in terms of their facilitation of timely clinical information...Full Text Available

2006-04-01

237

Cathepsin B facilitates Autophagy mediated apoptosis in SPARC Overexpressed Primitive Neuroectodermal Tumor cells

Psychopathic individuals are generally unresponsive to motivational and emotional cues that facilitate behavioral regulation. A putative mechanism for this deficiency is Gray’s (1971)...Full Text Available

2010-10-01

238

Bacterial Artificial Chromosome Mutagenesis Using Recombineering

Medulloblastoma and neuroblastoma belong to a group of neoplasms designated as primitive neuroectodermal tumors (PNETs). Secreted Protein, Acidic and Rich in Cysteine (SPARC) is a matrix-associated...Full Text Available

2010-10-01

239

BUBL LINK: Library organisations

Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. BACs are large enough...Full Text Available

2011-01-01

240

An unsupervised classification method for inferring original case locations from low-resolution disease maps

...6 Resource type: documents MmIT: Multimedia Information and Technology Group Group aiming to facilitate communication between CILIP members interested in multimedia developments in the library and information science field. Visits to places of interest like museums and archives are frequently organised, and an email discussion list ...

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241

An integrative multi-dimensional genetic and epigenetic strategy to identify aberrant genes and pathways in cancer

BackgroundWidespread availability of geographic information systems software has facilitated the use of disease mapping in academia, government and private sector. Maps that display...Full Text Available

242

Aggregation Substance Promotes Adherence, Phagocytosis, and Intracellular Survival of Enterococcus faecalis within Human Macrophages and Suppresses Respiratory Burst

BackgroundGenomics has substantially changed our approach to cancer research. Gene expression profiling, for example, has been utilized to delineate subtypes of cancer, and facilitated...Full Text Available

243

Ab binding alters gene expression in Cryptococcus neoformans and directly modulates fungal metabolism

The aggregation substance (AS) of Enterococcus faecalis, encoded on sex pheromone plasmids, is a surface-bound glycoprotein that mediates aggregation between bacteria thereby facilitating...Full Text Available

2000-09-01

244

17?-estradiol regulation of T-type calcium channels in gonadotropin-releasing hormone neurons

Abs facilitate humoral immunity via the classical mechanisms of opsonization, complement activation, Ab-dependent cellular cytotoxicity, and toxin/viral neutralization. There is also evidence that some...Full Text Available

2010-04-01

245

Cloning of Drosophila transcription factor Adf-1 reveals homology to Myb oncoproteins.

T-type calcium channels are responsible for generating low-threshold spikes that facilitate burst firing and neurotransmitter release in neurons. GnRH neurons exhibit burst firing, but the underlying...Full Text Available

2009-08-26

246

UHRF1, a modular multi-domain protein, regulates replication-coupled crosstalk between DNA methylation and histone modifications

The Drosophila sequence-specific DNA binding protein, Adf-1, is capable of activating transcription of the alcohol dehydrogenase gene, Adh, and is implicated in the transcriptional control of other developmentally regulated genes. We have cloned the cDNA encoding Adf-1 by generating specific DNA probes deduced from partial amino acid sequence of the protein. Several cDNA clones encoding an extended open reading frame were isolated from a phage lambda library. The complete amino acid sequence of Adf-1 deduced from the longest cDNA reveals structural similarities to the putative helix-turn-helix DNA binding motif of Myb and Myb-related proteins. DNA sequence analysis of genomic clones and Northern blot analysis of mRNA suggest that Adf-1 is a single-copy gene encoding a 1.9-kb transcript. Purified recombinant Adf-1 expressed in Escherichia coli binds specifically to Adf-1 recognition sites and activates ...

1992-01-15

247

Radioadapted chicken embryo cells: challenge specificity and alterations in higher-order DNA structure

Cytosine methylation in DNA is a major epigenetic signal, and plays a central role in propagating chromatin status during cell division. However the mechanistic links between DNA methylation and histone methylation are poorly understood. A multi-domain protein UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) is required for DNA CpG maintenance methylation at replication forks, and mouse UHRF1-null cells show enhanced susceptibility to DNA replication arrest and DNA damaging agents. Recent data demonstrated that the SET and RING associated (SRA) domain of UHRF1 binds hemimethylated CpG and flips 5-methylcytosine out of the DNA helix, whereas its tandom tudor domain and PHD domain bind the tail of histone H3 in a highly methylation sensitive manner. We hypothesize that UHRF1 brings the two components (histones and DNA) carrying appropriate markers (on the tails of H3 and hemimethylated CpG sites) ready to be assembled into a nucleosome after ...

2009-01-01

248

Morusin induces apoptosis and suppresses NF-#kappa#B activity in human colorectal cancer HT-29 cells

Radioadapted chicken embryo cells (X-irradiation in ovo with 10 cGy at the 14th day of development with priming periods of 24 h) were treated in vitro by challenge doses of 14 different DNA- and/or chromatin-interactive agents, including X-rays. A decrease in the cellular damage, as measured by scheduled DNA synthesis, was only observed with X-irradiation. Sedimentation of nucleoids as well as viscosity of alkaline lysates from ethidium bromide (0.35-400 {mu}g/ml)-, vovobiocin (125-1800 {mu}g/ml)-, and hyperthermia (30 min at 43 and 45 )-treated cells suggest a higher tendency of radioadapted cells to undergo positive DNA supercoiling. When DNA from adapted and non-adapted chicken embryo cells was used as substrate, neither its digestion by DNase I nor the inhibition of DNase I activity by various DNA-interactive agents was changed in primed cells. From the previous investigations as well as from the present results it is concluded that an increase of tightening of ...

1996-03-01

249

Application of the histone deacetylase inhibitors for the treatment of endometriosis: histone modifications as pathogenesis and novel therapeutic target.

Morusin is a pure compound isolated from root bark of Morusaustralis (Moraceae). In this study, we demonstrated that morusin significantly inhibited the growth and clonogenicity of human colorectal cancer HT-29 cells. Apoptosis induced by morusin was characterized by accumulation of cells at the sub-G_1 phase, fragmentation of DNA, and condensation of chromatin. Morusin also inhibited the phosphorylation of IKK-#alpha#, IKK-#beta# and I#kappa#B-#alpha#, increased expression of I#kappa#B-#alpha#, and suppressed nuclear translocation of NF-#kappa#B and its DNA binding activity. Dephosphorylation of NF-#kappa#B upstream regulators PI3K, Akt and PDK1 was also displayed. In addition, activation of caspase-8, change of mitochondrial membrane potential, release of cytochrome c and Smac/DIABLO, and activation of caspase-9 and -3 were observed at the early time point. Downregulation in the expression of Ku70 and XIAP was exhibited afterward. Caspase-8 or wide-ranging ...

2008-07-18

250

Poncirin promotes osteoblast differentiation but inhibits adipocyte differentiation in mesenchymal stem cells.

BACKGROUND Accumulating evidence suggests that various epigenetic aberrations play definite roles in the pathogenesis of endometriosis. We investigated the histone acetylation status in endometriosis and the application of the histone deacetylase inhibitors (HDACIs) for the treatment of endometriosis. METHODS The levels of acetylated histones in the endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs) were evaluated. The effects of the HDACIs on cell proliferation, the cell cycle, apoptosis of ECSCs and NESCs, and the expression of genes related to these cellular events were investigated. The effects of HDACIs on histone acetylation in chromatin of the promoter region of the cell cycle regulatory genes in ECSCs were also investigated. RESULTS The acetylated histone levels were significantly lower in ECSCs than in NESCs (P endometriosis and that HDACIs reactivated epigenetically silenced genes, resulting in the suppression of cell ...

2011-06-29

251

NADP Regulates the Yeast GAL Induction System

Poncirin, flavanone glycoside, isolated from the fruit of Poncirus trifoliata, has anti-bacterial and anti-inflammatory activities. In this study, the effects of poncirin on the differentiation of mesenchymal stem cells were investigated. The C3H10T1/2 mesenchymal stem cells and primary bone marrow mesenchymal stem cells were studied. In the C3H10T1/2 cells, poncirin prevented adipocyte differentiation, as demonstrated by inhibition of cytoplasm lipid droplet accumulation and peroxisome proliferator-activating receptor-? (PPAR-?) and CCAAT-enhancer-binding protein-? (C/EBP-?) mRNA expression. By contrast, poncirin enhanced the expression of the key osteogenic transcription factors, runt-related transcription factor 2 (Runx2) and transcriptional coactivator with PDZ-binding motif (TAZ). Poncirin also enhanced expression of the osteogenic marker genes including alkaline phosphatase (ALP) and osteocalcin (OC). Poncirin ...

2011-05-01

252

Expression profiles of PtrLOS2 encoding an enolase required for cold-responsive gene transcription from trifoliate orange

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial ...

2008-01-01

253

British Library Electronic Table of Contents (United Kingdom)

Low expression of osmotically responsive genes 2 (LOS2) encodes an enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) that converts 2-phospho-D-glycerate (PGA) to phosphoenolpyruvate (PEP) in the glycolytic pathway in Arabidopsis. Meanwhile, it is a transcriptional activator of cold-responsive gene, negatively controlling the expression of STZ/ZAT10, a zinc finger transcriptional repressor of cold-responsive gene from Arabidopsis. A novel LOS2 gene, designated PtrLOS2 (GenBank accession number GQ144341), was isolated from trifoliate orange [Poncirus trifoliata (L.) Raf.]. The PtrLOS2 cDNA is 1 662 bp in length with a 1 338 bp open reading frame (ORF), encoding a deduced 445 amino acid residue protein with a predicted molecular mass of 47.79 kDa and an isoelectric point of 5.54. The ded...

2011-01-01

254

Ectopic expression of wild-type or a dominant-negative mutant of transcription factor NTF-1 disrupts normal Drosophila development.

Analysis of the 5{prime} region of PMS2 reveals heterogeneous transcripts and a novel overlapping gene

The Drosophila melanogaster tissue-specific transcription factor NTF-1 was originally identified in vitro as a protein that could bind to and activate transcription from the Dopa decarboxylase (Ddc) gene. A structure-function analysis of NTF-1 led to the identification of a discrete amino-terminal activation domain. Here, we report that an NTF-1 mutant lacking the activation domain acts as a trans-dominant inhibitor of NTF-1 activation in tissue culture cells by forming inactive heterodimers with the full-length protein. Ectopically expressing this dominant-negative protein or the full-length protein in developing Drosophila embryos leads to dire developmental consequences. Overexpressing the trans-dominant NTF-1 leads to lethality, while overexpressing full-length NTF-1 results in both lethality and morphogenetic defects. Our results suggest that both the activity and the regulation of NTF-1 are critical for viability and proper development of ...

1993-11-15

255

Wnt/b-catenin signal pathway stabilizes APP intracellular domain (AICD) and promotes its transcriptional activity

The PMS2 gene encodes a protein that is involved in DNA mismatch repair and is mutated in a subset of patients with hereditary nonpolyposis colon cancer (HNPCC). The previously published PMS2 cDNA sequence lack an upstream in-frame stop codon preceding the presumptive initiating methionine. To evaluate the 5` terminus of the PMS2 coding region further, we isolated additional cDNA clones, RT-PCR products, and the corresponding 5` genomic segment of the PMS2 locus. The PMS2 gene transcripts were found to have heterogeneous but colinear 5` termini, one of which contained an in-frame termination codon preceding the initiating methionine. In addition, a novel gene encoding a 34.5-kDa polypeptide was found to initiate transcriptionally within PMS2 from the opposite strand. 23 refs., 5 figs., 2 tabs.

1995-09-20

256

British Library Electronic Table of Contents (United Kingdom)

Amyloid precursor protein (APP), a key protein in pathogenesis of Alzheimer's disease (AD), is a type I transmembrane protein which can be cleaved by b- and g-secretase to release the amyloidogenic b-amyloid peptides (Ab) and the APP intracellular domain (AICD). While Ab has been widely believed to initiate pathogenic cascades culminating AD, the physiological functions and regulations of AICD remain elusive. In present study, endogenous AICD was demonstrated to be increased by canonical Wnt signal. Instead of due to g-secretase activity, enhanced AICD expression was found due to the increased protein stability by Wnt/b-catenin. b-Catenin was demonstrated to be an associating partner of AICD, capable of promoting AICD mediated transcriptional activity. Investigation by AICD mutants proved ...

2011-01-01

257

The hippo pathway in biological control and cancer development

British Library Electronic Table of Contents (United Kingdom)

Abstract The Hippo pathway is an evolutionally conserved protein kinase cascade involved in regulating organ size in vivo and cell contact inhibition in vitro by governing cell proliferation and apoptosis. Deregulation of the Hippo pathway is linked to cancer development. Its first core kinase Warts was identified in Drosophila more than 15 years ago, but it gained much attention when other core components of the pathway were identified 8 years later. Major discoveries of the pathway were made during past several years. The core kinase components Hippo, Salvador, Warts, and Mats in the fly and Mst1/2, WW45, Lats1/2, and Mob1 in mammals phosphorylate and inactivate downstream transcriptional co-activators Yorkie in the fly, Yes-associated protein (YAP) and transcriptional co-activator with ...

2011-01-01

258

The Significance of PITX2 Overexpression in Human Colorectal Cancer

British Library Electronic Table of Contents (United Kingdom)

Purpose The paired-like homeodomain transcription factor 2 (PITX2) gene encodes a transcription factor controlled by the WNT/Dvl/CTNNB1 and Hedgehog/TGFB pathways in the pathogenesis of colorectal cancer (CRC). Although PITX2 is reportedly involved in various functions, including tissue development by controlling cell growth, its significance in CRC remains unclear. We report our findings regarding abnormal PITX2 expression in human CRC. Methods PITX2 expression was evaluated in 5 human CRC cell lines and 92 primary CRC samples. Cell growth was evaluated after inhibition of PITX2 expression or after exogenous introduction of PITX2. Results PITX2 expression was seen in all the five CRC cell lines. The study of tissue samples indicated that PITX2 expression was significantly higher in cancer...

2011-01-01

259

Selective changes of retroelement expression in human prostate cancer

British Library Electronic Table of Contents (United Kingdom)

Retroelements constitute a large part of the human genome. These sequences are mostly silenced in normal cells, but genome-wide DNA hypomethylation in cancers might lead to their re-expression. Whether this re-expression really occurs in human cancers is largely unkown. We therefore investigated expression and DNA methylation of several classes of retroelements in human prostate cancer tissues and cell lines by quantitative reverse transcription-polymerase chain reaction and pyrosequencing, respectively. The most striking finding was strong and generalized increased expression of the HERV-K_22q11.23 provirus in cancers, including de novo expression of a spliced accessory Np9 transcript in some tumors. In parallel, DNA methylation in the long terminal repeat (LTR) decreased. Conversely, HER...

2011-01-01

260

Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma

Hypoxia decreases sclerostin expression and increases Wnt signaling in osteoblasts

Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells. These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes. This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of cervical carcinoma.

1989-01-01

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261

British Library Electronic Table of Contents (United Kingdom)

Mutations in sclerostin function or expression cause sclerosing bone dysplasias, involving decreased antagonism of Wnt/Lrp5 signaling. Conversely, deletion of the VHL tumor suppressor in osteoblasts, which stabilize HIF-a isoforms and thereby enables HIF-a/b-driven gene transcription, increases bone mineral content and cross-sectional area compared to wild-type controls. We examined the influence of cellular hypoxia (1% oxygen) upon sclerostin expression and canonical Wnt signaling. Osteoblasts and osteocytes cultured under hypoxia revealed decreased sclerostin transcript and protein, and increased expression and nuclear localization of activated b-catenin. Similarly, both hypoxia and the hypoxia mimetic DFO increased b-catenin gene reporter activity. Hypoxia and its mimetics increased exp...

2010-01-01

262

Forkhead box protein P1 as a downstream target of transforming growth factor-b induces collagen synthesis and correlates with a more stable plaque phenotype

British Library Electronic Table of Contents (United Kingdom)

Objective: Atherosclerosis is an inflammatory disease, modulated by plaque stabilizing and de-stabilizing cell populations such as infiltrating monocytes and vascular smooth muscle cells (vSMCs). Transcription factors regulating proliferation and differentiation of atherosclerosis relevant cell types are of interest in this context. The forkhead box transcription factor FoxP1 modulates monocyte differentiation. We studied FoxP1 expression in atherosclerotic tissue, correlated FoxP1 expression with plaque characteristics and identified associations between FoxP1 and plaque proteins. Methods: 116 Atherosclerotic plaques from carotid endarterectomy samples were histologically classified (fibrous, fibroatheromatous, atheromatous) and subjected to semi-quantitative protein analysis. Macrophage,...

2011-01-01

263

Effects of ozone on gene expression and lipid peroxidation in adults and larvae of the red flour beetle (Tribolium castaneum)

British Library Electronic Table of Contents (United Kingdom)

Ozone has in recent years been increasingly investigated for its potential use in the control of insect pests of stored cereals. Ozone is a powerful oxidizing agent that can react directly, or via production of reactive oxygen species, with proteins, DNA and double bonds of polyunsaturated fatty acids (PUFA). The aim of the present study was to investigate the mode of action in ozone toxicity using the red flour beetle, Tribolium castaneum (Herbst), as a relevant model. Transcription of focal genes thought to be involved in protection against ozone, and repair of cellular damage caused by ozone exposure, was studied together with the composition of storage lipid fatty acids and membrane phospholipid fatty acids in order to detect lipid peroxidation. Contrary to expectations, transcription ...

2011-01-01

264

Effect of postharvest water deficit stress on gene expression in heads of broccoli (Brassica oleracea var. italica)

British Library Electronic Table of Contents (United Kingdom)

Harvested plant organs such as heads of broccoli (Brassica oleracea L. var. italica) experience a range of stresses that can lead to premature reduction in quality and eventual senescence. Understanding plant responses to stress may open up novel opportunities to extend postharvest life. One of the first stresses experienced by harvested organs is likely to be water deficit stress since severance of the vascular system halts the normal flux of water into the tissue. For broccoli branchlets with their cut ends held in water, transcriptome analysis based on hybridization of broccoli floret mRNA to a heterologous Arabidopsis microarray revealed that the transcript abundance of 431 genes reliably changed within 48h of harvest. Of these, transcripts of 146 genes increased and 34 genes decreased...

2011-01-01

265

ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

BzpF is a CREB-like transcription factor that regulates spore maturation and stability in Dictyostelium

Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. We have created a database of evolutionary conserved regions (ECRs) in vertebrate genomes entitled ECRbase that is constructed from a collection of pairwise vertebrate genome alignments produced by the ECR Browser database. ECRbase features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a collection of promoters in all vertebrate genomes presented in the database. The database also contains a collection of annotated transcription factor binding sites (TFBS) in all ECRs and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and two pufferfish genomes. It is freely accessible at http://ECRbase.dcode.org.

2006-08-08

266

British Library Electronic Table of Contents (United Kingdom)

The cAMP response element-binding protein (CREB) is a highly conserved transcription factor that integrates signaling through the cAMP-dependent protein kinase A (PKA) in many eukaryotes. PKA plays a critical role in Dictyostelium development but no CREB homologue has been identified in this system. Here we show that Dictyostelium utilizes a CREB-like protein, BzpF, to integrate PKA signaling during late development. bzpF^- mutants produce compromised spores, which are extremely unstable and germination defective. Previously, we have found that BzpF binds the canonical CRE motif in vitro. In this paper, we determined the DNA binding specificity of BzpF using protein binding microarray (PBM) and showed that the motif with the highest specificity is a CRE-like sequence. BzpF is necessary to ...

2011-01-01

267

Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root

British Library Electronic Table of Contents (United Kingdom)

Glycyrrhiza glabra root is one of the common traditional Chinese medicines and used as flavoring and sweetening agents for tobaccos, chewing gums, candies, toothpaste and beverages. While glycyrrhizin is one of the main components in the extract of G. glabra root and has been characterized, the other components have not been well characterized. The mechanism of growth activation of breast cancer MCF-7 cells, including the activation of Erk1/2 and Akt, and the transcriptional regulation of estrogen-responsive genes, was examined by means of sulforhodamine B, luciferase reporter gene, real-time RT-PCR and Western blotting assays after the induction of the cells with the extract of G. glabra root. The extract has similar activity to that induced by 17b-estradiol (E2), although glycyrrhizin di...

2007-01-01

268

c-Jun N-terminal Kinase 2 Regulates Multiple Receptor Tyrosine Kinase Pathways in Mouse Mammary Tumor Growth and Metastasis

bHLH-PAS family transcription factor methoprene-tolerant plays a key role in JH action in preventing the premature development of adult structures during larval-pupal metamorphosis

c-Jun N-terminal kinase 2 (JNK2) isoforms are transcribed from the jnk2 gene and are highly homologous with jnk1 and jnk3 transcriptional products....Full Text Available

2011-01-01

269

Wake-up-call, a lin-52 paralogue, and Always early, a lin-9 homologue physically interact, but have opposing functions in regulating testis-specific gene expression

The biological actions of juvenile hormones are well studied; they regulate almost all aspects of an insect’s life. However, the molecular actions of these hormones are not well understood....Full Text Available

2008-07-01

270

Unusual heterogeneity of the 5'-termini of human adenovirus type 2 early region E2 mRNA.

A conserved multi-subunit complex (MybMuvB, MMB), regulates transcriptional activity of many different target genes in Drosophila somatic cells. A paralogous complex, tMAC, controls...Full Text Available

2011-07-15

271

The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors

The 5'-terminal structures of human adenovirus type 2 (Ad2) early region 2 (E2) mRNA were investigated. The E2 transcription unit has several interesting properties, including the presence of a TATA-like...Full Text Available

1984-12-11

272

The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth

In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3′- and 5′-ends of 261 yeast genes by...Full Text Available

2010-08-01

273

Polyunsaturated Fatty Acids Selectively Suppress Sterol Regulatory Element-binding Protein-1 through Proteolytic Processing and Autoloop Regulatory Circuit*

The Myc oncoprotein is a potent inducer of cell growth, cell cycle progression, and apoptosis. While many direct Myc target genes have been identified, the molecular determinants of Myc’s transcriptional...Full Text Available

2007-03-01

274

Polyadenylated H3 histone transcripts and H3 histone variants in alfalfa.

Sterol regulatory element-binding protein (SREBP)-1 is a key transcription factor for the regulation of lipogenic enzyme genes in the liver. Polyunsaturated fatty acids (PUFA) selectively suppress hepatic...Full Text Available

2010-04-09

275

PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1

Histone H3 mRNAs were found in polyA(+) fractions of total RNA prepared from alfalfa plants, calli and somatic embryos. The sequence analysis of cDNAs revealed the presence of a polyA tail on independent...Full Text Available

1989-04-25

276

Nuclear receptor co-repressor SMRT regulates mitochondrial oxidative metabolism and mediates aging related metabolic deterioration

LIMD1 is a tumour suppressor gene (TSG) down regulated in ∼80% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG...Full Text Available

2011-04-06

277

NPM-ALK and the JunB transcription factor regulate the expression of cytotoxic molecules in ALK-positive, anaplastic large cell lymphoma

SummaryThe transcriptional co-repressor SMRT utilizes two major receptor interacting domains (RID1 and RID2) to mediate nuclear receptor (NR) signaling through epigenetic modification....Full Text Available

2010-12-01

278

Anaplastic lymphoma kinase-positive, anaplastic large cell lymphoma (ALK+ ALCL) is an aggressive non-Hodgkin lymphoma of T/null immunophenotype that is most prevalent in children and young adults. The...Full Text Available

2011-02-15

279

Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli

BackgroundUsing a transcriptional profiling approach, we recently identified myeloid-related protein-8/14 (MRP-8/14) to be expressed by platelets during acute MI....Full Text Available

2008-01-01

280

MicroRNAs Regulate Human Hepatocyte Nuclear Factor 4?, Modulating the Expression of Metabolic Enzymes and Cell Cycle*

BackgroundThe large sensitivity, high reproducibility and essentially unlimited dynamic range of real-time PCR to measure gene expression in complex samples provides the opportunity...Full Text Available

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281

Mice With a Deletion in the Gene for CCAAT/Enhancer-Binding Protein ? are Protected Against Diet-Induced Obesity

Hepatocyte nuclear factor (HNF) 4α is a key transcription factor regulating endo/xenobiotic-metabolizing enzymes and transporters. We investigated whether microRNAs are involved in the regulation...Full Text Available

2010-02-12

282

Lytic HSV-1 infection induces the multifunctional transcription factor Early Growth Response-1 (EGR-1) in rabbit corneal cells

The CCAAT/enhancer-binding protein β (C/EBPβ) is required for adipocyte differentiation and maturation. We have studied the role of the transcription factor, C/EBPβ,...Full Text Available

2007-01-01

283

Insect-Induced Conifer Defense. White Pine Weevil and Methyl Jasmonate Induce Traumatic Resinosis, de Novo Formed Volatile Emissions, and Accumulation of Terpenoid Synthase and Putative Octadecanoid Pathway Transcripts in Sitka Spruce1[w

BackgroundHerpes simplex virus type-1 (HSV-1) infections can cause a number of diseases ranging from simple cold sores to dangerous keratitis and lethal encephalitis. The interaction...Full Text Available

284

Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.

Stem-boring insects and methyl jasmonate (MeJA) are thought to induce similar complex chemical and anatomical defenses in conifers. To compare insect- and MeJA-induced terpenoid responses, we analyzed...Full Text Available

2005-01-01

285

Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids...Full Text Available

1984-07-01

286

Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation

We have identified another Drosophila GTP-binding protein (G protein) alpha subunit, dGq alpha-3. Transcripts encoding dGq alpha-3 are derived from alternative splicing of the dGq alpha locus previously...Full Text Available

1995-12-05

287

Gene rearrangement and radiation carcinogenesis

BackgroundEstrogen receptors alpha (ERα) and beta (ERβ) are transcription factors (TFs) that mediate estrogen signaling and define the hormone-responsive phenotype...Full Text Available

288

Forkhead Box Transcription Factor FOXO3a Regulates Estrogen Receptor Alpha Expression and Is Repressed by the Her-2/neu/Phosphatidylinositol 3-Kinase/Akt Signaling Pathway

The development of leukemia and thyroid cancer is characterized by activation of the abl oncogene and ret oncogene, respectively. In order to clarify the relationship between these gene aberrations and radiation, the pro-myelogenous leukemia-derived cell line HL60 and the thyroid cancer-derived cell line 8505C, were irradiated in vitro with 100Gy of X-rays. RNA was then extracted from 10"8 cells of the respective cell lines and examined by the reverse transcription PCR method for rearrangements of abl and ret genes. Five kinds of positive bands were observed in the HL-60 cells irradiated with 100Gy of X-ray. Similarly, six positive bands were also observed in the 8505C cells irradiated with 100Gy. In vitro X-irradiation activation of oncogenes found in radiation induced cancers imply that gene rearrangement by X-rays is involved in the development of malignant tumors. Furthermore, in an experiment to detect radiation effects in A-bomb survivors, RNA was extracted ...

1993-11-01

289

Evolutionary Constraints Acting on DDX3X Protein Potentially Interferes with Rev-Mediated Nuclear Export of HIV-1 RNA

The expression status of the estrogen receptor alpha (ERα) and that of the epidermal growth factor receptor Her-2/neu frequently correlate inversely in breast cancers. While ERα-dependent...Full Text Available

2004-10-01

290

Evolution of Hox Post-Transcriptional Regulation by Alternative Polyadenylation and MicroRNA Modulation Within 12 Drosophila Genomes.

Differential host-pathogen interactions direct viral replication in infected cells. In HIV-1 infected cells, nuclear export of viral RNA transcripts into cellular cytoplasm is governed by interaction...Full Text Available

291

Different genetic requirements for anterior RNA localization revealed by the distribution of Adducin-like transcripts during Drosophila oogenesis.

Hox genes encode a family of transcriptional regulators that operate differential developmental programs along the anteroposterior axis of bilateral animals. Regulatory changes affecting Hox gene expression are believed to have been crucial for the evolution of animal body plans. In Drosophila melanogaster, Hox expression is post-transcriptionally regulated by microRNAs (miRNAs) acting on target sites located in the 3' untranslated regions (3'UTRs) of Hox mRNAs. Notably, recent work has shown that during D. melanogaster development Hox genes produce mRNAs with variable 3'UTRs (short and long forms) in different sets of tissues as a result of alternative polyadenylation; importantly, Hox short and long 3'UTRs contain very different target sites for miRNAs. Here, we use a computational approach to explore the evolution of Hox 3'UTRs treated with especial regard to miRNA regulation. Our work is focused on the 12 Drosophila species for which ...

2011-03-24

292

Deriving four functional anti-HIV siRNAs from a single Pol III-generated transcript comprising two adjacent long hairpin RNA precursors

The proteins encoded by polar-localized mRNAs play an important role in cell fate specification along the anteroposterior axis of the Drosophila embryo. The only maternally synthesized mRNA known previously...Full Text Available

1993-03-15

293

Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

Several different approaches exist to generate expressed RNA interference (RNAi) precursors for multiple target inhibition, a strategy referred to as combinatorial (co)RNAi. One such approach makes...Full Text Available

2010-10-01

294

Computational identification of developmental enhancers:conservation and function of transcription factor binding-site clustersin drosophila melanogaster and drosophila psedoobscura

The aim of this study was to investigate cumulus expansion, nuclear maturation and expression of connexin 43, cyclooxygenase-2 and FSH receptor transcripts in equine cumuli oophori during in vivo and...Full Text Available

295

Cleavage of tRNA within the mature tRNA sequence by the catalytic RNA of RNase P: implication for the formation of the primer tRNA fragment for reverse transcription in copia retrovirus-like particles.

Background The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. Results We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated to those of ...

2004-08-06

296

Characterization of the human lipoprotein lipase (LPL) promoter: Evidence of two cis-regulatory regions, LP-[alpha] and LP-[beta] of importance for the differentation-linked induction of the LPL gene during adipogenesis

The retrovirus-like particles of Drosophila are intermediates of retrotransposition of the transposable element copia. In these particles, a 39-nucleotide-long fragment from the 5' region of Drosophila...Full Text Available

1990-10-01

297

BosR (BB0647) Controls the RpoN-RpoS Regulatory Pathway and Virulence Expression in Borrelia burgdorferi by a Novel DNA-Binding Mechanism

When preadipocytes differentiate into adipocytes, several differentiation-linked genes are activated. Lipo-protein lipase (LPL) is one of the first genes induced during this process. To investigate early events in adipocyte development, we have focused on the transcriptional activation of the LPL gene. For this purpose, we have cloned and fused different parts of intragenic and flanking sequences with a chloramphenicol acetyltransferase reporter gene. Transient transfection experiments and DNase I hypersensitivity assays indicate that several positive as well as negative elements contribute to transcriptional regulation of the LPL gene. When reporter gene constructs were stably introduced into preadipocytes, we were able to monitor and compare the activation patterns of different promoter deletion mutants at selected time points representing the process of adipocyte development. We could delimit two cis-regulatory elements important for gradual ...

1992-10-01

298

Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.

In Borrelia burgdorferi (Bb), the Lyme disease spirochete, the alternative σ factor σ⁵⁴ (RpoN) directly activates transcription of another alternative σ...Full Text Available

2011-02-01

299

Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root.

The Epstein-Barr virus nuclear antigen (EBNA I) present in latently infected cells is encoded in a 2-kilobase exon contained in the BamHI K viral genomic fragment. This exon is, however, found within...Full Text Available

1985-12-01

300

A divergent Tbx6-related gene and Tbx6 are both required for neural crest and intermediate mesoderm development in Xenopus

Glycyrrhiza glabra root is one of the common traditional Chinese medicines and used as flavoring and sweetening agents for tobaccos, chewing gums, candies, toothpaste and beverages. While glycyrrhizin is one of the main components in the extract of G. glabra root and has been characterized, the other components have not been well characterized. The mechanism of growth activation of breast cancer MCF-7 cells, including the activation of Erk1/2 and Akt, and the transcriptional regulation of estrogen-responsive genes, was examined by means of sulforhodamine B, luciferase reporter gene, real-time RT-PCR and Western blotting assays after the induction of the cells with the extract of G. glabra root. The extract has similar activity to that induced by 17beta-estradiol (E(2)), although glycyrrhizin did not show such an activity. Moreover, the estrogen receptor alpha-dependent neurite outgrowth induced by the extract was similar to that by E(2), whereas glycyrrhizin had no ...

2007-06-24

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301

A Role for Set1/MLL-Related Components in Epigenetic Regulation of the Caenorhabditis elegans Germ Line

AbstractT-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus....Full Text Available

2010-04-01

302

A Nonparametric Mean-Variance Smoothing Method to Assess Arabidopsis Cold Stress Transcriptional Regulator CBF2 Overexpression Microarray Data

The methylation of lysine 4 of Histone H3 (H3K4me) is an important component of epigenetic regulation. H3K4 methylation is a consequence of transcriptional activity, but also has been shown to contribute...Full Text Available

2011-03-01

303

3-Methyl-3-deazaadenine, a stable isostere of N3-methyl-adenine, is efficiently bypassed by replication in vivo and by transcription in vitro.

Microarray is a powerful tool for genome-wide gene expression analysis. In microarray expression data, often mean and variance have certain relationships. We present a non-parametric mean-variance smoothing...Full Text Available

304

(?) Epigallocatechin gallate suppresses the differentiation of 3T3-L1 preadipocytes through transcription factors FoxO1 and SREBP1c

The goal of the present work was to determine the impact of N3-methyladenine (3-mA), an important lesion generated by many environmental agents and anticancer drugs, on in vivo DNA replication and in vitro RNA transcription. Due to 3-mA chemical instability, the stable isostere 3-methyl-3-deazaadenine (3-m-c(3)A) was site specifically positioned into an oligodeoxynucleotide. The oligomer was, then incorporated into a vector system that is rapidly converted to ssDNA inside yeast cells and requires DNA replication opposite the lesion for plasmid clonal selection. For control purposes, an adenine or a stable apurinic/apyrimidinic (AP)-lesion was placed at the same site. The presence of each lesion in the oligonucleotide was confirmed by MALDI-TOF analysis. Plasmids were then transfected into yeast cells. While the AP-site dramatically reduced plasmid replication in all strains, the 3-m-c(3)A had a slight effect in the rad30 background which significantly increased ...

2011-06-14

305

Scaffolding collaborative technical writing with procedural facilitation and synchronous discussion

Tea catechin is one of the compounds that are closely related to obesity and insulin sensitivity. In order to determine the effect of catechin on adipocyte differentiation, we treated 3T3-L1 preadipocytes...Full Text Available

2010-06-01

306

British Library Electronic Table of Contents (United Kingdom)

With the advent of computer technology, researchers and instructors are attempting to devise computer support for effective collaborative technical writing. In this study, a computer-supported environment for collaborative technical writing was developed. This system (Process-Writing Wizard) provides process-oriented scaffolds and a synchronous online chat room to facilitate real-time collaborative writing practice. It allows multiple students to work synchronously on collaborative writing tasks via the Internet. It also helps develop collaborative writing strategies, such as creating team agendas, brainstorming, creating team outlines, and generating team articles. An experiment was conducted to examine the effect of the system on EFL (English as a Foreign Language) students? collaborativ...

2011-01-01

307

Ion processing element with composite media

Change in number and activation of androgen receptor-immunoreactive cells in the medial amygdala in response to chemosensory input

An ion processing element employing composite media disposed in a porous substrate, for facilitating removal of selected chemical species from a fluid stream. The ion processing element includes a porous fibrous glass substrate impregnated by composite media having one or more active components supported by a matrix material of polyacrylonitrile. The active components are effective in removing, by various mechanisms, one or more constituents from a fluid stream passing through the ion processing element. Due to the porosity and large surface area of both the composite medium and the substrate in which it is disposed, a high degree of contact is achieved between the active component and the fluid stream being processed. Further, the porosity of the matrix material and the substrate facilitates use of the ion processing element in high volume applications where it is desired to effectively process a high volume flows.

2009-03-24

308

British Library Electronic Table of Contents (United Kingdom)

In many species social behaviors are dependent on integration of chemosensory and hormonal cues. Many chemosensory stimuli are detected by the vomeronasal organ, which projects to many regions that contain steroid receptors, including the medial amygdala. In male hamsters, testosterone is known to acutely increase in response to chemosensory stimulation, and can facilitate sexual behavior by direct action within the medial amygdala. Conspecific stimuli activate the anterior (MeA) and posterior (MeP) medial amygdala, while heterospecific stimuli activate only MeA. Chemosensory stimuli with different social significance differentially activate the dorsal and ventral subdivisions of MeA and MeP. Therefore, it is likely that steroids differentially facilitate stimulation of the medial amygdala...

2011-01-01

309

Automatic calculation of bathymetry for coastal hydrodynamic models

British Library Electronic Table of Contents (United Kingdom)

A methodology is proposed to facilitate the construction of gridded bathymetry data for the use of hydrodynamic models on the continental shelf. It relies on the carrying out of three successive tasks: (1) automatic selection of records of better quality among multiple sets of overlapping data; (2) elimination of data points located on land; and (3) taking into account the shoreline as bathymetric data. Algorithms are proposed to perform sorting of the records according to their quality as well as masking by the coastline. The suggested method facilitates the updating of bathymetry data and optimizes their use. It enables automatic execution of all the tasks and building of new digital bathymetry models in a few hours, without action from the operator. The method has been tested many times...

2011-01-01

310

Status Report on the Creation of a Preliminary Data Model and Dictionary for a New Petrologic Database

Scyl1 Facilitates Nuclear tRNA Export in Mammalian Cells by Acting at the Nuclear Pore Complex

A preliminary database has been developed that will allow mineralogy and bulk-rock geochemical information to be managed under configuration control and facilitate electronic querying. The database is currently developed in Microsoft Access as a collection of tables, views, and entry forms. Each field and table has been described in a data dictionary.

2008-06-30

311

Role of Intimin-Tir Interactions and the Tir-Cytoskeleton Coupling Protein in the Colonization of Calves and Lambs by Escherichia coli O157:H7

Scyl1 is an evolutionarily conserved N-terminal protein kinase-like domain protein that plays a role in COP1-mediated retrograde protein trafficking in mammalian cells. Furthermore, loss of Scyl1 function...Full Text Available

2010-07-15

312

Intimin facilitates intestinal colonization by enterohemorrhagic Escherichia coli O157:H7; however, the importance of intimin binding to its translocated receptor (Tir) as opposed to...Full Text Available

2006-01-01

313

Pumped storage plants

Proton beam therapy control system

Pumping is one of the means for storing vast quantities of energy. This article analyses its principles and ways of implementation with emphasis on its double aspect, e.g., on the one hand, off-peak power is transferred to the periods of heaviest load and is thus valorized, even when taking into account losses due to poor efficiency of operations in the pumping and turbine modes, and on the other hand, plants have great flexibility in operation, faciliting the permanent fitting of generation to demand.

1982-09-01

314

Plugging indicator for controlling the sodium quality. Indicateur de bouchage pour le controle de la qualite du sodium

A tiered communications architecture for managing network traffic in a distributed system. Communication between client or control computers and a plurality of hardware devices is administered by agent and monitor devices whose activities are coordinated to reduce the number of open channels or sockets. The communications architecture also improves the transparency and scalability of the distributed system by reducing network mapping dependence. The architecture is desirably implemented in a proton beam therapy system to provide flexible security policies which improve patent safety and facilitate system maintenance and development.

2010-09-21

315

Oil cable pumping plant. Pumpestasjon

This device has and head-race pipe with an external part and a coaxial inner part. A measuring head is mounted movable between these 2 parts and has at least a thermocouple and a mobile grid for varying the section and the sodium flow to facilitate the impurities dissolution. This ensemble is completed by a longitudinal body containing the main parts of the indicator and surrounded by a thermal exchanger to cool the sodium.

1991-09-20

316

Mapping of Ethiopian higher education institutions on clean energy

The invention deals with a pumping plant for oil filled power cables. An air driven piston type pump is used as primary pump. A PLS (Programmable Logic control System) is used to control the oil flow to the cable(s). Improvements of the pump includes means for ensuring that the pump piston is operated also at low pressure and flow, and means for sealing off the piston rod to ensure maximum life of seals, to facilitate detection of possible leakages and to avoid contamination of the cable oil. 3 figs.

1988-12-27

317

Learning Online Social Support: An Investigation of Network Information Technology Based on UTAUT

Norad commissioned Econ Poeyry to map teaching and research activities and capacity related to clean energy in selected Ethiopian universities. The mapping identified challenges and opportunities with the aim of facilitating future intervention by the Ethiopian Government and donors to help improve the energy sector development of the country. The report covered the government-owned universities of Bahir Dar, Mekelle, Jimma, Arba Minch and Addis Ababa. The mapping was based on a questionnaire and on interviews at each university. (Author)

2011-04-15

318

British Library Electronic Table of Contents (United Kingdom)

ABSTRACT Extending the unified theory of acceptance and use of technology (UTAUT) model, this study postulates a model of online social support. The model is empirically tested using data from undergraduates in Taiwan regarding their usage of instant messaging (IM). The test results indicate that all model paths are significant, except that the path between online social support and facilitating conditions is insignificant. This study offers limitations and implications.

2008-01-01

319

LIQUID NITROGEN CRYOSTAT TO FACILITATE ISOTHERMAL ANNEALING STUDIES ON METALS AFTER HEAVY-PARTICLE BOMBARDMENT

Knowledge-based society, peer production and the common good

A cryostat is described that was developed for irradiating a number of small metal specimens with a high beamcurrent of heavy particles at liquid nitrogen temperature. The specimens, which are mounted on a block, are taken out of the cryostat after irradiation and subsequently annealed in a temperature bath. The progress of annealing is followed by measuring the change in electrical resistivity of the specimens in liquid helium. (auth)

1964-01-01

320

DEFF Research Database (Denmark)

This article investigates the societal conditions that might help the establishment of peer-to-peer modes of production. First, the context within which such a new model is emerging - the neoliberal knowledge-based-societies - is described, and its shortcomings unveiled; and second, a robust argument is provided for the moral legitimation of an alternative societal vision, including two structural policies that are likely to facilitate the establishment and further development of peer-to-peer practices.

2009-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

Inspection, a practised art?; Revisionen, eine geuebte Praxis?

Enzyme discovery in hybrid aspen for fibre engineering

Revisions at steam turbines should present a problem-free procedure due to the long use of these machines. Changes in the market result in difficulties during the inspection work. The shortage of qualified personnel and manufacturing capacities are the cause for these difficulties. Important procedures of revisions are described in detail in the VGB regulations. Direct contacts between the customers and the suppliers facilitate the expirations substantially. In the case of good planning and under consideration of the well-known regulations, a revision can be accomplished to the satisfaction of customers and suppliers.

2008-07-01

322

Environmental Research Database

DescriptionEnzymes are natural catalysts that are used increasingly to replace environmentally harmful chemicals and to achieve better catalytic selectivity during pulp and paper processing. The main objective of this project is to identify novel plant enzymes for deeper understanding of the process of fibre formation and for future improvement of the quality parameters of wood fibres. Specific modification of the fibre characteristics will facilitate their use as raw materials for the current pulp and pap [continued...

2005-01-31

323

Education in Indonesia: Coping with Challenges in the Third Millennium.

EcoEarth.Info Environment Links: Ocean/Information

Describes the Indonesian system of education of Islamic schooling, secular education, and out-of-school education. The provision of 9-year universal basic education is planned by 2004. The national plan challenges the education system to facilitate the change in Indonesia's economic structure from an agriculturally based system to one more dependent on manufacturing and service industries. (SLD)

1999-12-01

324

Dynamic performance testing of control valves

... 01, 2009 | Rate It Marine Technology Society https://www.mtsociety.org/home.aspx emphasizes the importance of marine technology as it applies to global issues, encourages marine technology education and facilitates the use of marine technology in managing marine resources Added: Mar. 11, 2010 |...

325

Development of a new secondary beam separator and a new gas-jet target at Kyushu University

Response of control valves plays an important role in the dynamics of the flow system as a whole. Knowledge of its transfer function would facilitate analysis of the behaviour of the system. This paper presents the application of Levy's complex curve fitting method for determination of the transfer function of control valves used in Liquid Zone Control System of 540 MWe PHWR. (author)

2005-02-01

326

Determination of progesterone for reproduction control in cows using a /sup 3/H radioimmunoassay. 1. Methods and choice of testing samples

In order to facilitate observations of low energy nuclear reactions, a new type recoil mass-separator together with a new gas-jet target system is being developed at the tandem accelerator facility in Kyushu University. The expected mass-resolving power of the separator is 220 for a solid angle of 10 msr and the practical thickness of the gas-jet target will exceed 0.1 atm#centre dot#cm for the light elements of H and He. (author).

1994-06-01

327

Computer tomography used in tumour diagnosis in children

For verification of cow fertility a /sup 3/H radioimmunoassay of progesterone in milk and blood plasma was developed. It is of high specificity and accuracy as well. Extraction of progesterone from milk was facilitated by application of alcohol. Suggested differences in milk and plasma progesterone levels between pregnant and nonpregnant cows could be revealed.

1984-01-01

328

Computed tomography of the chest: A teaching file

Computer tomography used in the diagnosis of tumours is described in the light of particularly great diagnostic difficulties in children. Computer tomography was applied in 12 cases. A high diagnostic value of this method was demonstrated, which makes possible a more accurate diagnosis of the position of the tumour, its size, and expanding tendency. The possibility of estimating healthy tissues and organs, and those with pathological changes on the basis of differences in their density with a detection threshold at 0.5% density difference facilitates not infrequently preoperative diagnosis raising the value of the method.

1981-01-01

329

As a self-teaching tool, this illustrated guide facilitates home study of the complexities of thoracic CT's. Over 400 illustrations and accompanying copy are used to provide a basis for understanding the CT appearances of disease processes and a comparison of CT lookalikes. It updates new and interesting developments in the format of case presentations.

1987-01-01

330

Annual report 1993-1994

S1 nuclease analysis of #alpha#-globin gene expression in preleukemic patients with acquired hemoglobin H disease after transfer to mouse erythroleukemia cells

The Atlantic Wind Test Site was established in 1980 as a laboratory to facilitate the evaluation and demonstration of wind energy systems and equipment. This annual report describes its organization and management, facilities, major projects, and the outlook for the future. Major projects include wind diesel; AWTS-IREQ collaboration; remote community data acquisition; lagerwey test program; Alaska Energy Authority Project; public education; Atlantic Orient Corporation 15/50 Project; and the soft VAWT project.

1994-12-31

331

Nuclear factor of activated T cell (NFAT) transcription proteins regulate genes involved in adipocyte metabolism and lipolysis

The loss of #alpha#-globin gene transcriptional activity rarely occurs as an acquired abnormality during the evolution of myeloproliferative disease or preleukemia. To test whether the mutation responsible for the loss of #alpha#-globin gene expression (hemoglobin H disease) in these patients is linked with the #alpha#-globin genes on chromosome 16, the authors transferred chromosome 16 from preleukemic patients with acquired hemoglobin H disease to mouse erythroleukemia cells and measured the transcriptional activity of the human #alpha#-globin genes. After transfer to mouse erythroleukemia cells, the expression of human #alpha#-globin genes from the peripheral blood or marrow cells of preleukemic patients with acquired hemoglobin H disease was similar to that of human #alpha#-globin genes transferred to mouse erythroleukemia cells from normal donors. These data showed that factor(s) in the mouse erythroleukemia cell can genetically complement ...

332

Genetic engineering of group 2 sigma factor SigE widely activates expressions of sugar catabolic genes in Synechocystis species PCC 6803.

NFAT involvement in adipocyte physiological processes was examined by treatment with CsA and/or GSK3{beta} inhibitors (Li{sup +} or TZDZ-8), which prevent or increase NFAT nuclear translocation, respectively. CsA treatment reduced basal and TNF{alpha}-induced rates of lipolysis by 50%. Adipocytes preincubated with Li{sup +} or TZDZ-8 prior to CsA and/or TNF{alpha}, exhibited enhanced basal rates of lipolysis and complete inhibition of CsA-mediated decreased rates of lipolysis. CsA treatment dramatically reduced the mRNA levels of adipocyte-specific genes (aP2, HSL, PPAR{gamma}, ACS and Adn), compared with control or TNF{alpha}-treatment, whereas Li{sup +} pretreatment blocked the inhibitory effects of CsA, and mRNA levels of aP2, HSL, PPAR{gamma}, and ACS were found at or above control levels. NFAT nuclear localization, assessed by EMSA, confirmed that CsA or Li{sup +} treatments inhibited or increased NFAT nuclear translocation, respectively. These results show that NFAT proteins in ...

2007-09-21

333

Boron-Dependent Degradation of NIP5;1 mRNA for Acclimation to Excess Boron Conditions in Arabidopsis.

Metabolic engineering of photosynthetic organisms is required for utilization of light energy and for reducing carbon emissions.Control of transcriptional regulators is a powerful approach for changing cellular dynamics, because a set of genes is concomitantly regulated. Here, we show that overexpression of a group 2 ? factor, SigE, enhances the expressions of sugar catabolic genes in the unicellular cyanobacterium, Synechocystis sp. PCC 6803. Transcriptome analysis revealed that genes for the oxidative pentose phosphate pathway and glycogen catabolism are induced by overproduction of SigE. Immunoblotting showed that protein levels of sugar catabolic enzymes, such as glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glycogen phosphorylase, and isoamylase, are increased. Glycogen levels are reduced in the SigE-overexpressing strain grown under light. Metabolome analysis revealed that metabolite levels of the TCA cycle and acetyl-CoA are ...

2011-07-11

334

Affinity purification of sequence-specific DNA binding proteins.

Boron (B) is an essential plant micronutrient that is toxic at higher levels. NIP5;1 is a boric acid channel required for B uptake and growth under B deficiency. Accumulation of the NIP5;1 transcript is upregulated under B deficiency in Arabidopsis thaliana roots. To elucidate the mechanism of regulation, the 5' untranslated region (UTR) of NIP5;1 was tested for its ability to confer B-dependent regulation using ?-glucuronidase and green fluorescent protein as reporters. This analysis showed that the 5' UTR was involved in NIP5;1 transcript accumulation in response to B conditions. We also found that high-B conditions trigger NIP5;1 mRNA degradation and that the sequence from +182 to +200 bp in the 5' UTR is required for this mRNA destabilization. In the nip5;1-1 mutant background, a NIP5;1 complementation construct without the 5' UTR produced high levels of mRNA accumulation, increased B concentrations in tissues, and reduced growth under ...

2011-09-01

335

Dispersion of Metals from Abandoned Mines and their Effect on Biota in the Methow River, Okanogan County, Washington: Final Report 2002-2003.

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This DNA is then covalently coupled to Sepharose CL-2B with cyanogen bromide to yield the affinity resin. A partially purified protein fraction is combined with competitor DNA and subsequently passed through the DNA-Sepharose resin. The desired sequence-specific DNA binding protein is purified because it preferentially binds to the recognition sites in the affinity resin rather than to the nonspecific competitor DNA in solution. For example, a protein fraction that is enriched for transcription factor Sp1 can be further purified 500- to 1000-fold by two sequential affinity chromatography steps to give Sp1 of an estimated 90% homogeneity with 30% yield. In addition, the use ...

1986-08-01

336

ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome

A study of mine-waste contamination effects on Methow River habitat on the eastern slopes of the north Cascade Mountains in Washington state, U.S.A., revealed impacts at ecosystem, community, population, individual, tissue, and cellular levels. Ore deposits in the area were mined for gold, silver, copper and zinc until the early 1950's, but the mines are now inactive. An above-and-below-mine approach was used to compare potentially impacted to control sites. The concentrations of eleven trace elements (i.e., Al, As, B, Ba, Cd, Cr, Cu, Mn, Pb, Se, and Zn) in Methow River sediments downstream from the abandoned mine sites were higher than background levels. Exposed trout and caddisfly larvae in the Methow River showed reduced growth compared to controls. Samples of liver from juvenile trout and small intestine from exposed caddisfly larvae were examined for evidence of metal accumulation, cytopathological change, and chemical toxicity. Morphological changes that are ...

2003-05-15

337

The arts of indigenous online dissent: Negotiating technology, indigeneity, and activism in the Cordillera

ATR-X (alpha-thalassemia/mental retardation, X-linked) syndrome is a human congenital disorder that causes severe intellectual disabilities. Mutations in the ATRX gene, which encodes an ATP-dependent chromatin-remodeler, are responsible for the syndrome. Approximately 50% of the missense mutations in affected persons are clustered in a cysteine-rich domain termed ADD (ATRX-DNMT3-DNMT3L, ADD{sub ATRX}), whose function has remained elusive. Here we identify ADD{sub ATRX} as a previously unknown histone H3-binding module, whose binding is promoted by lysine 9 trimethylation (H3K9me3) but inhibited by lysine 4 trimethylation (H3K4me3). The cocrystal structure of ADD{sub ATRX} bound to H3{sub 1-15}K9me3 peptide reveals an atypical composite H3K9me3-binding pocket, which is distinct from the conventional trimethyllysine-binding aromatic cage. Notably, H3K9me3-pocket mutants and ATR-X syndrome mutants are defective in both H3K9me3 binding and localization at ...

2011-07-19

338

British Library Electronic Table of Contents (United Kingdom)

The online communicative environment is expected to revolutionize political discourse as it expands to cover underrepresented groups and ideas. In this platform, marginalized groups such as indigenous communities from the developing world can articulate claims, strategically mobilize and participate in the forms of meaning-making that constitute them. However, there is skepticism on the actual value of online spaces in effecting agency in an internet-mediated environment. Using James Scott's notion of 'hidden transcripts' and Andrew Feenberg's 'democratic rationalization of technology', the paper explores strategic approaches and historical, social, and political conditions embedded in the construction, negotiation, and transformation of indigenous online activist media. In-depth interview...

2012-01-01

339

Regulation of Redd1 Expression by Hypoxia

Reciprocal regulation of the neural and innate immune systems

Redd1, a recently discovered stress-response gene, is regulated by hypoxia via hypoxia-inducible factor 1 (HIF-1) and by DNA damage via p53/p63; however, the signaling pathway by which its expression is induced by hypoxia has not been elucidated. We demonstrated that the up-regulation of Redd1 transcription by hypoxia and high cell density (HCD) depends on cooperation between Sp1 and HIF-1#alpha# downstream of the PI3K/Akt pathway.

2006-05-25

340

British Library Electronic Table of Contents (United Kingdom)

Innate immune responses are regulated by microorganisms and cell death, as well as by a third class of stress signal from the nervous and endocrine systems. The innate immune system also feeds back, through the production of cytokines, to regulate the function of the central nervous system (CNS), and this has effects on behaviour. These signals provide an extrinsic regulatory circuit that links physiological, social and environmental conditions, as perceived by the CNS, with transcriptional 'decision-making' in leukocytes. CNS-mediated regulation of innate immune responses optimizes total organism fitness and provides new opportunities for therapeutic control of chronic infectious, inflammatory and neuropsychiatric diseases.

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

Moving toward personalized cell-based interventions for adrenal cortical disorders: Part 1 - Adrenal development and function, and roles of transcription factors and signaling proteins

British Library Electronic Table of Contents (United Kingdom)

Transdifferentiation of an individual's own cells into functional differentiated cells to replace an organ's lost function would be a personalized approach to therapeutics. In this two part series, we will describe the progress toward establishing functional transdifferentiated adrenal cortical cells. In this article (Part 1), we describe adrenal development and function, and discuss genes involved in these processess and selected for use in our pilot studies of transdifferentiation that are presented in the second article (Part 2).

2011-01-01

342

Interpreting Mammalian Evolution using Fugu Genome Comparisons

In vitro evaluation of herpes simplex virus type 1 thymidine kinase reporter system in dynamic studies of transcriptional gene regulation

Comparative sequence analysis of the human and the pufferfish Fugu rubripes (fugu) genomes has revealed several novel functional coding and noncoding regions in the human genome. In particular, the fugu genome has been extremely valuable for identifying transcriptional regulatory elements in human loci harboring unusually high levels of evolutionary conservation to rodent genomes. In such regions, the large evolutionary distance between human and fishes provides an additional filter through which functional noncoding elements can be detected with high efficiency.

2004-04-02

343

Transcriptional Profiling Analysis of the Global Regulator NorG, a GntR-Like Protein of Staphylococcus aureus.

The herpes simplex virus type 1 thymidine kinase (HSV1-TK) reporter system is being used to directly and indirectly monitor therapeutic gene expression, immune cell trafficking and protein-protein interactions in various living animals. However, the issues of HSV1-TK enzyme stability in living cells and whether this reporter system is optimal for dynamic studies of gene expression events in genetic imaging have not be addressed. The purpose of the present study was to evaluate the application of this reporter system in dynamic studies of transcriptional gene regulation. To achieve this purpose, we established two tetracycline-inducible murine sarcoma cell lines, tetracycline-turn-off HSV1-tk-expressing cell line (NG4TL4/tet-off-HSV1-tk) and tetracycline-turn-off Luc-expressing cell line (NG4TL4/tet-off-Luc), to create an artificially regulated gene expression model in vitro. The dynamic transcriptional events mediating a series of doxycycline ...

2006-07-15

344

Transcription regulation of the vegf gene by the BMP/Smad pathway in the angioblast of zebrafish embryos

The GntR-like protein NorG has been shown to affect Staphylococcus aureus genes involved in the resistance to quinolones and ?-lactams, such as those encoding the NorB and AbcA transporters. To identify the target genes regulated by NorG, we carried out transcriptional profiling assays using S. aureus RN6390 and its isogenic norG::cat mutant. Our data showed that NorG positively affected the transcription of global regulators mgrA, arlS, and sarZ. The three putative drug efflux pump genes most positively affected by NorG were the NorB efflux pump (5.1-fold), the MmpL-like protein SACOL2566 (5.2-fold), and the BcrA-like drug transporter SACOL2525 (5.7-fold). The S. aureus predicted MmpL protein showed 53% homology with the MmpL lipid transporter of Mycobacterium tuberculosis, and the putative SACOL2525 protein showed 87% homology with the bacitracin drug transporter BcrA of Staphylococcus hominis. Two pump genes most negatively affected by NorG ...

2011-09-01

345

Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-B1

Vascular endothelial growth factor (VEGF) is a mitogen that is critically involved in vasculogenesis, angiogenesis, and hematopoiesis. However, what and how transcription factors participate in the regulation of vegf gene expression are not fully understood. Here we report the cloning and sequencing of the zebrafish vegf promoter which revealed that the promoter contains a number of bone morphogenetic protein (BMP)-activated Smad binding elements (SBE), implicating Smad1 and Smad5 in the regulation of BMP-induced expression of vegf. Electrophoretic mobility shift assays of adding recombinant Smad proteins to the SBE-containing DNA oligonucleotides that represent portions of zebrafish vegf promoter resulted in mobility shift of the oligonucleotides. These changes demonstrate potential interactions between Smad1/5 and the vegf promoter. Reporter activity assays using the wild-type or SBE-deleted vegf promoters to drive the luciferase reporter gene expression revealed ...

2005-04-01

346

Friend Spleen Focus-Forming Virus Activates the Tyrosine Kinase sf-Stk and the Transcription Factor PU.1 to Cause a Multi-Stage Erythroleukemia in Mice.

Mesenchymal stem cells (MSCs) are a potential source of smooth muscle cells (SMCs) for constructing tissue-engineered vascular grafts. However, the details of how specific combinations of vascular microenvironmental factors regulate MSCs are not well understood. Previous studies have suggested that both mechanical stimulation with uniaxial cyclic strain and chemical stimulation with transforming growth factor {beta}1 (TGF-{beta}1) can induce smooth muscle markers in MSCs. In this study, we investigated the combined effects of uniaxial cyclic strain and TGF-{beta}1 stimulation on MSCs. By using a proteomic analysis, we found differential regulation of several proteins and genes, such as the up-regulation of TGF-{beta}1-induced protein ig-h3 (BGH3) protein levels by TGF-{beta}1 and up-regulation of calponin 3 protein level by cyclic strain. At the gene expression level, BGH3 was induced by TGF-{beta}1, but calponin 3 was not significantly regulated by mechanical strain or TGF-{beta}1, ...

2009-10-12

347

Deficiency of the housekeeping gene hypoxanthine-guanine phosphoribosyltransferase (HPRT) dysregulates neurogenesis.

HEMATOLOGICAL MALIGNANCIES IN HUMANS TYPICALLY INVOLVE TWO TYPES OF GENETIC CHANGES: those that promote hematopoietic cell proliferation and survival (often the result of activation of tyrosine kinases) and those that impair hematopoietic cell differentiation (often the result of changes in transcription factors). The multi-stage erythroleukemia induced in mice by Friend spleen focus-forming virus (SFFV) is an excellent animal model for studying the molecular basis for both of these changes. Significant progress has been made in understanding the molecular basis for the multi-stage erythroleukemia induced by Friend SFFV. In the first stage of leukemia, the envelope protein encoded by SFFV interacts with and activates the erythropoietin (Epo) receptor and the receptor tyrosine kinase sf-Stk in erythroid cells, causing their Epo-independent proliferation, differentiation and survival. In the second stage, SFFV integration into the Sfpi1 locus activates the myeloid ...

2010-10-11

348

Nuclear Reactor Sharing Program

Neuronal transcription factors play vital roles in the specification and development of neurons, including dopaminergic (DA) neurons. Mutations in the gene encoding the purine biosynthetic enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT) cause the resulting intractable and largely untreatable neurological impairment of Lesch-Nyhan disease (LND). The disorder is associated with a defect in basal ganglia DA pathways. The mechanisms connecting the purine metabolic defect and the central nervous system (CNS) phenotype are poorly understood but have been presumed to reflect a developmental defect of DA neurons. We have examined the effect of HPRT deficiency on the differentiation of neurons in the well-established human (NT2) embryonic carcinoma neurogenesis model. We have used a retrovirus expressing a small hairpin RNA (shRNA) to knock down HPRT gene expression and have examined the expression of a number of transcription factors ...

2009-08-11

349

Keynote address. Division 1. Energy and economic development

The Ohio State University Research Reactor (OSURR) is licensed to operate at a maximum power level of 500 kW. A pool-type reactor using flat-plate, low enriched fuel elements, the OSURR provides several experimental facilities including two 6-inch i.d. beam ports, a graphite thermal column, several graphite-isotope-irradiation elements, a pneumatic transfer system (Rabbit), various dry tubes, and a Central Irradiation Facility (CIF). The core arrangement and accessibility facilitates research programs involving material activation or core parameter studies. The OSURR control room is large enough to accommodate laboratory groups which can use control instrumentation for monitoring of experiments. The control instrumentation is relatively simple, without a large amount of duplication. This facilitates opportunities for hands-on experience in reactor operation by nuclear engineering students making reactor parameter measurements. For neutron ...

1994-09-01

350

Effects of intersegmental transfers on target location by proteins

This paper was presented at the opening plenary session. As the end of the century approaches, environmental protection is one of the most significant global issues which will shape our energy path and economic development pattern. In face of environmental challenges, we will have to take into account environmental factors in the economic development process itself. This requires the pursuit of sustainable economic development, a process which may involve strengthened energy conservation, improved efficiency and switching to less carbon intensive energy sources. Energy inefficiency, technological scarcity and financial vulnerability in developing countries may best be resolved by changing their economic and market structures to facilitate relevant investments. However, effective reform of the energy industry in developing economies and economies in transition will also require international cooperation in terms of transfer of technology, capital, know-how and ...

1995-12-31

351

CERN Document Server

We study a model for a protein searching for a target, using facilitated diffusion, on a DNA molecule confined in a finite volume. The model includes three distinct pathways for facilitated diffusion: (a) sliding - in which the protein diffuses along the contour of the DNA (b) jumping - where the protein travels between two sites along the DNA by three-dimensional diffusion, and finally (c) intersegmental transfer - which allows the protein to move from one site to another by transiently binding both at the same time. The typical search time is calculated using scaling arguments which are verified numerically. Our results suggest that the inclusion of intersegmental transfer (i) decreases the search time considerably (ii) makes the search time much more robust to variations in the parameters of the model and (iii) that the optimal search time occurs in a regime very different than that found for models which ignore intersegmental transfers. The ...

2008-01-01

352

Zephyr: A secure Internet process to streamline engineering

Triplet superconductors as the basis for solid-state quantum computing

Lawrence Livermore National Laboratory (LLNL) is implementing an Internet-based process pilot called `Zephyr` to streamline engineering and commerce using the Internet. Major benefits have accrued by using Zephyr in facilitating industrial collaboration, speeding the engineering development cycle, reducing procurement time, and lowering overall costs. Programs at LLNL are potentializing the efficiencies introduced since implementing Zephyr. Zephyr`s pilot functionality is undergoing full integration with Business Systems, Finance, and Vendors to support major programs at the Laboratory.

1998-05-12

353

Tolerance analysis of a phase space beam analyzer

We propose triplet superconductors, such as ruthenates, as prospective materials for qubit construction. The vectorial nature of the order parameter in triplet superconductors makes it conceptually easy to estimate the performance of the qubits. The Cooper condensate of pairs in triplet superconductors has all the attributes of Bose-Einstein condensates and should facilitate long decoherence times for these qubits, relative to other vectorial schemes for qubits, such as small ferromagnets. There are other benefits, which the superconducting state provides for requirements such as entanglement between qubits via the proximity effect, etc. We consider these benefits in detail, although our consideration is only preliminary and further experimental and theoretical research will undoubtedly introduce correctives.

2003-12-01

354

The inventor?s role: was Schumpeter right?

The phase space beam analyzer is a measurement instrument that is applied in laser technology to perform analyses of the spatial and angular distribution of rays. We are interested in this instrument as a means to characterize non-coherent light sources. In this context, a closer look at the tolerances of this optical instrument was considered useful. Having a so-called quadrupole lens as a key element, the phase space beam analyzer is a device that features anamorphic optical properties. To describe these anamorphic properties, recurrence was made to a description by extended ray-transfer matrices. This formalism allows for an analysis of the alignment tolerances of the phase space beam analyzer and facilitates a study of the sensitivities of the instrument. The analysis is complemented using numerical ray tracing.

2007-09-01

355

British Library Electronic Table of Contents (United Kingdom)

According to Schumpeter, the creative process of economic development can be divided into the stages of invention, innovation (commercialization) and imitation. Each stage is associated with specific skills. This paper examines whether Schumpeter?s assertion was correct, i.e. whether the invention and innovation stages should be undertaken by different agents. In addition, we examine whether there is a rationale for the Schumpeterian entrepreneur to include the inventor in the commercialization process. Combining the abilities of the entrepreneur and the inventor may serve to facilitate customer adaptation, strengthen knowledge transfers and reduce uncertainty, thereby expanding market opportunities. Based on a unique database covering Swedish patents granted to individuals and small firms...

2010-01-01

356

The green power option to gain market advantage

Surface doping of conjugated polymers by graphene oxide and its application for organic electronic devices

Deregulation of the electric power industry in Alberta holds the prospect of providing greater choice for customers. One such option will be `green power` which is provided by renewable energy technologies. The energy produced would be without environmental impacts such as air emissions, water pollution, flooding, thermal emissions or waste by-products. Wind power, small scale hydro power, photovoltaics and biomass technologies have been termed as `green power`. The choices that customers make will not be focused on price alone. Market forces are emerging to demand reduction in emissions which will result in a market for `green energy` products and facilitate achieving goals for a sustainable future.

1997-03-01

357

Satellite power systems (SPS) concept definition study (exhibit D). Volume 5: systems engineering/integration research and technology. Final report

Surface doping of conjugated polymers is realized by depositing a thin layer of graphene oxide (GO) on top of the polymers. The high proton density and the unique 2D structure of GO facilitate the protonic surface doping of conjugated polymers to achieve high conductivities. This finding represents a new strategy for improving charge transport across the metal/conjugated polymer interface to achieve much improved performance in organic solar cells. (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

2011-04-26

358

Recycling of PTFE by means of ionizing rays

Guidelines and ground rules followed in the development of requirements for the SPS are presented. Development planning objectives are specified in each of these areas, and evolutionary SPS program scenarios are described for the various concepts studied during the past one year contract. Program descriptions are presented as planning packages of technical tasks, and schedule phasing. Each package identifies the ground based technology effort that will facilitate SPS definitions, designs, development, and operations.

1981-03-01

359

Palliation of radiation-related mucositis

By treatment with beta or gamma rays, PTFE waste is converted to high-grade PTFE fine powder. High-molecular weight PTFE is degraded to low-molecular weight PTFE. Due to this the density, the degree of crystallinity, and the melt flow index are increased, which enhances the brittleness of the material. Irradiation of PTFE thus facilitates milling and makes the material pourable and free-flowing. Milled PTFE fine powder, lie PTFE plastic, combines temperature resistance, weather fastness, and chemical stability with good slip behaviour and antiadhesive properties. Areas of application for fine powder include additives for plastics, printing inks, nonstick coatings, and antifriction lacquers. (orig.).

1994-01-01

360

Optimized hydrogen piston engines

Oral mucositis associated with head and neck radiotherapy can substantially hinder completion of cancer therapy. Alleviation of this often severe stomatitis can provide enhanced patient comfort and facilitate appropriate care. A double-blind format was used in a pilot project to measure, against a control rinse, the effectiveness of an oral rinse consisting of hydrocortisone, nystatin, tetracycline, and diphenhydramine in controlling radiation-related mucositis. A combination of clinical evaluation and patient responses to a questionnaire was used to judge the results of the topical medications. Patients using the experimental medication developed less mucositis than did patients in the control group.

1990-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Online tax payment systems as an emergent aspect of governmental transformation

Hydrogen piston engines can be simultaneously optimized for improved thermal efficiency and for extremely low emissions. Using these engines in constant-speed, constant-load systems such as series hybrid-electric automobiles or home cogeneration systems can result in significantly improved energy efficiency. For the same electrical energy produced, the emissions from such engines can be comparable to those from natural gas-fired steam power plants. These hydrogen-fueled high-efficiency, low-emission (HELE) engines are a mechanical equivalent of hydrogen fuel cells. HELE engines could facilitate the transition to a hydrogen fuel cell economy using near-term technology.

1994-05-10

362

British Library Electronic Table of Contents (United Kingdom)

The achievement of governmental transformation through the use of electronically delivered services is a worthy goal that requires significant planning and research to achieve. In order to reach transformational paradigm shifts in governmental operation, it will first be necessary to understand and optimize present governmental e-Service provisions. Of these, the revenue function of taxation is paramount. This paper describes factors related to the use and acceptance by accounting professionals of information technology intended to facilitate electronic tax filing systems. Though tested in the context of governmental tax management systems in Turkey, our findings on the use and acceptance of e-Tax systems are relevant and applicable to a great number of nations and contexts as the ongoing ...

2011-01-01

363

Microbiological Transformations of Radionuclides in the Subsurface

Meier associates and Pacific Northwest Laboratory staff exchange: Transfer of corrosion monitoring expertise to assess and develop in-line inspection tools for corrosion control

Microorganisms are ubiquitous in subsurface environments although their populations sizes and metabolic activities can vary considerably depending on energy and nutrient inputs. As a result of their metabolic activities and the chemical properties of their cell surfaces and the exopolymers they produce, microorganisms can directly or indirectly facilitate the biotransformation of radionuclides, thus altering their solubility and overall fate and transport in the environment. Although biosorption to cell surfaces and exopolymers can be an important factor modifying the solubility of some radionuclides under specific conditions, oxidation state is often considered the single most important factor controlling their speciation and, therefore, environmental behavior.

2010-01-04

364

Magnetic resonance imaging of the pediatric brain

Staff exchanges, such as the one described in this report, are intended to facilitate communication and collaboration among scientists and engineers at DOE laboratories, in US industry, and academia. During the past 5 years, PNL has developed prototype instrumentation to automate the data collection required for electrochemical determination of corrosion rates and behavior of materials in various electrically conductive environments. The last version is labeled the Sentry 100 prototype corrosion data scanner. Applications include these in the pulp and paper industry and at hazardous waste sites.

1995-04-01

365

International cooperation - Information Centre - Research & Innovation - European Commission

The atlas presents sequences of MRI sections parallel to the orbito-meatal plane in children from birth through the age of sixteen years. Each child was studied horizontally and sagitally and three-dimensional brain images were reconstructed to facilitate accurate identification of sulci and gyri. The images show crucial aspects of brain development such as the constancy of the brain stem and primitive brain from birth onward; the development of the telencephalon, characterized by deepening of sulci and growth of the cerebral cortex surface; and the different stages of white matter myelinization.

366

Interference phenomena at the elastic collision of atoms with formation of the Feshbach resonance in the presence of laser radiation field

... Removal of this layer facilitates the unreeling of cocoons into long strands of silk comparable to those derived from the domesticated mulberry silkworm (Bombyx mori, B. mori). The research was funded in part by the SABIP ('Silks as biomimetic ideals for polymers) project, which has clinched a European Research Council (ERC) grant worth almost EUR 2.3 million under the EU's Seventh Framework Programme (FP7). Presented in the journal Biomacromolecules, the findings could lead to the development of new silk industries in ...

367

British Library Electronic Table of Contents (United Kingdom)

Resonant scattering of atoms with formation of the Feshbach resonance in the presence of a laser radiation coupling the levels of two bound atoms (a molecule) is considered. The laser field leads to a second resonance in scattering and broadening of resonances, which facilitates the possibility of experimental observation of asymmetry of the total scattering cross-section arising because of interference between resonant and potential scatterings. The effects associated with interference of the two channels of decay of a bound system of two atoms (a molecule) in the laser field are studied. An expression is obtained for the scattering length in collision of two cold atoms in the field of laser radiation.

2011-01-01

368

Intelligent Interaction with Knowledge Associates

Information compendium of the Mexican Energy Sector

The Knowledge Associates for Novel Intelligence (KANI) system provides a collection of automated 'associates' to actively support and participate in the information analysis task. In this paper, we describe the Information Integration Associate (IIA), which facilitates analyst interaction with underlying KANI reasoning and extraction associates and provides an interactive representation of the analytic process. We focus on features of the IIA that guide communication between the user and the KANI system in a way that ensures mutual understanding of the analytic task being performed, the capabilities of the system to assist the user, and the status of any reasoning and extraction conducted on behalf of the user by the KANI system.

2006-01-29

369

In-situ polymerization of vinyl pyrrolidinone on nylon 66 by gamma radiation

This is the first report about a relevant and compiled information on the Mexican Energy Sector. It was realized in one volume by the University Energy Program of the National Autonomous University of Mexico and it comprises information for the period 1996-1997. In the chapter 1 there are presented the numbers of hydrocarbon reserves. Chapter 2 treats about production, events and its results. Likewise chapter 3 shows information about the typical Installations for petroleum, gas and electricity including through of the nuclear energy. In the chapter 4, named Energy Consumption, is presented a National energy balance for 1996 and, finally there are a sections about the prices, the rates and a glossary to facilitate its understanding. (Author)

370

Improvement of muscle strenght independently of analgesic effect following spinal cord stimulation. A case report.

The polymerization of vinyl pyrrolidinone on nylon 66, with the assistance of aqueous phenol and formic acid, was investigated by mutual and post irradiation procedures. Both solvents were found to greatly facilitate the polymerization with the post irradiation procedure, whereas neither presented any advantage with the mutual irradiation procedure. Tensile properties of the modified fibers were affected in similar ways by both the irradiation procedures and solvents in that the only notable changes were the somewhat higher extensions at yield and at break. The moisture regain values of the treated yarns and fabrics were increased up to three-fold. Surface morphology of the modified fibers was revealed by SEM.

1984-10-01

371

Human dietary intakes of trace elements: A global literature survey mainly for the period 1970-1991: 1. Data listings and sources of information

Spinal cord stimulation (SCS) is frequently used for relief of chronic benign pain resistant to conservative therapies. Clinical practice suggests, at least in patients with failed back surgery syndrome (FBSS), the possibility that SCS significantly improves motor performances. We present here the case of a 41-years-old female patient with FBSS, who showed a clear improvement in muscle strength after SCS, persisting at 6-months follow-up. We speculate that the electrical stimulation of posterior columns could potentiate the caudal, segmental spinal reflexes resulting in a facilitation of motoneurons activation. PMID:16175150

2004-12-01

372

Functionalized luminescent oxide nanoparticles for sodium channel imaging at the single molecule level

A database has been compiled on human dietary intakes of trace elements by populations groups living in various countries. The main sources of information used were (1) publications in the open scientific literature, and (2) data submitted to the IAEA in response to a questionnaire. The database contains 1,758 intake values covering 35 elements and 47 countries. In this report the data are listed in various ways to facilitate identification of intake values for any specified country, element and population group. 4 refs, 5 tabs.

373

Financing `Orbital Power and Light Inc`

Lanthanide-ion doped oxide nanoparticles were functionalized for use as fluorescent biological labels. These nanoparticles are synthesized directly in water which facilitates their functionalization, and are very photostable without emission intermittency. Nanoparticles functionalized with guanidinium groups act as artificial toxins and specifically target sodium channels. They are individually detectable in cardiac myocytes, revealing a heterogeneous distribution of sodium channels. Functionalized oxide nanoparticles appear as a novel tool particularly well adapted to long-term single-molecule tracking.

2005-04-01

374

Enhancing employability in the "ME generation"

Financing space solar power generation programmes has several inherent difficulties. Such large-scale projects are usually easily defined with an expected economic return, construction time, useful service life and defined management structure. As yet, solar power satellites for power generation possess none of these as the idea exists conceptually and the technology still needs to be developed. Methods for overcoming these uncertainties and securing financial backing are described. Venture capital could finance a research/marketing firm. US Government support through a cost plus short-term monopoly to facilitate private debt financing could provide an answer. (UK)

1998-06-01

375

British Library Electronic Table of Contents (United Kingdom)

Purpose - The purpose of this paper is to place all of the contributions to this special issue into a theoretical framework and to highlight the role that the so-called "information age mindset" has in the facilitation of employability skills. Design/methodology/approach - The paper discusses the major themes of this special issue. Findings - Undergraduate students do see the importance of technological innovation in the classroom but they see the development of experiential or work-based skills to be more important. Practical implications - Future curriculum design should consider the expectations and attitudes of the modern day undergraduate student to ensure that potential employability is maximised. Originality/value - The findings are placed into the wider context of the emerging fiel...

2010-01-01

376

Engineering guides for estimating cover material thickness and volume for uranium mill tailings

Developmental facial paralysis: A review

Five nomographs have been prepared that facilitate the estimation of cover thickness and cover material volume for the Uranium Mill Tailing Remedial Action Program. Key parameters determined include the cover thickness with either a surface radon flux or a boundary radon air concentration criterion and the total volume of cover material required for two different treatments of the edge slopes. Also included in the engineering guide are descriptions and representative values for the radon source term, the diffusion coefficients and the key meteorological parameters. 16 refs., 7 figs., 2 tabs.

1982-09-01

377

British Library Electronic Table of Contents (United Kingdom)

The purpose of this study is to clarify the confusing nomenclature and pathogenesis of Developmental Facial Paralysis, and how it can be differentiated from other causes of facial paralysis present at birth. Differentiating developmental from traumatic facial paralysis noted at birth is important for determining prognosis, but also for medicolegal reasons. Given the dramatic presentation of this condition, accurate and reliable guidelines are necessary in order to facilitate early diagnosis and initiate appropriate therapy, while providing support and counselling to the family. The 30 years experience of our center in the management of developmental facial paralysis is dependent upon a thorough understanding of facial nerve embryology, anatomy, nerve physiology, and an appreciation of well...

2011-01-01

378

Contribution to the compensation of extra high voltage transmission lines

Computational Chemistry Study of Solvents for Carbon Dioxide Absorption

An improved method to design suitable compensating schemes for extra high voltage transmission lines is presented. The technique is general and effective and will greatly facilitate the design of the optimal compensation scheme for extra high voltage transmission lines. The technique is illustrated for a typical 500 kV system. The equations developed here for voltage maxima and minima along the transmission line eliminate the need for point-to-point computation of the voltage profile. The controversy existing in the literature over the problem of making a choice between pi and tee configurations has been removed and it is established with a mathematical proof that the tee configuration requires compensating elements of lower ratings.

1987-04-01

379

Common envelope evolution

Absorption with aqueous amine solvents is at present the most viable technology for CO{sub 2} capture. While this is a proven technology, efforts are ongoing to improve it in order to make it a more attractive technology for large scale use to reduce CO{sub 2} emissions. Finding solvents with better properties is one approach to improving the technology. In this thesis methods in computational chemistry are used to improve the understanding of the chemistry of CO{sub 2} absorption in amine-water systems. The work is also intended to provide models that can be used to predict the performance of new solvents. Such predictive models are intended to facilitate the screening for new solvents

2005-08-15

380

British Library Electronic Table of Contents (United Kingdom)

The common envelope phase of binary star evolution plays a central role in many evolutionary pathways leading to the formation of compact objects in short period systems. Using three dimensional hydrodynamical computations, we review the major features of this evolutionary phase, focusing on the conditions that lead to the successful ejection of the envelope and, hence, survival of the system as a post common envelope binary. Future hydrodynamical calculations at high spatial resolution are required to delineate the regime in parameter space for which systems survive as compact binary systems from those for which the two components of the system merge into a single rapidly rotating star. Recent algorithmic developments will facilitate the attainment of this goal.

2010-01-01

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381

Coal Corporation of Victoria. Annual report 1989-90. [Australia - Victoria

Application aspects of the static frequency converter system

The Coal Corporation of Victoria's mission is to initiate, facilitate and co-ordinate brown coal-based developments, other than for electricity generation, to provide maximum long-term benefits to Victoria including new jobs, new technology, new investment and new products, within sound environmental parameters. The marketing of brown coal products is vigorously undertaken to provide the financial and business base for the Corporation. This report contains a review of the Corporation's activities and achievements during 1989/90.

1990-01-01

382

Advances in molecular biology: impact on rotavirus vaccine development.

The use of reversible pump/turbine sets in pumped storage has created a requirement for rapid reversibility. Softstart technology, based on static frequency converters (SFC) has evolved to such an extent that it is now the best choice for serving this purpose, both from a technical and from an economic point of view. The objective of our paper is to give the owners of pumped storage plants and those persons responsible for the selection, the operation or the maintenance of the electrical equipment the necessary insight into the SFC functions, features and all other application related aspects, to facilitate their decision making process. Case studies are included.

1995-12-31

383

Addition of methyl cellulose enema to double-contrast barium imaging of sigmoid diverticulosis

The first candidate rotavirus vaccine was a live attenuated oral vaccine made by the classical empirical method of serial passage of virus in tissue culture cells. Current tetravalent vaccine candidates that are in the final stages of efficacy testing in the United States were made by genetic reassortment. This article briefly highlights how advances in the basic understanding of the molecular biology of rotaviruses have facilitated vaccine development. New approaches for second-generation vaccines and improvements in vaccine efficacy based on further exploitation of the tools and knowledge of rotavirus molecular biology and pathogenesis are discussed. PMID:8752289

1996-09-01

384

A solution to add digital signatures to medical images according to the DICOM standard: embedded systems

Double-contrast barium enema has a reduced sensitivity in patients with severe sigmoid diverticulosis. Therefore a carboxy methyl cellulose enema was employed after the conventional double-contrast examination in 15 patients with sigmoid diverticulosis. A significant increase in lumen diameter and a superior removal of barium residue from the diverticulas facilitated the interpretation of the sigmoid loops. Conclusion: The addition of methyl cellulose enema to double-contrast barium imaging improves diagnostic imaging in diverticulosis by expanding the lumen and emptying the diverticulas. (orig.).

1997-01-01

385

A research agenda to examine the efficacy and relevance of the Transtheoretical Model for physical activity behavior

Radiology departments often underestimate the importance of protecting medical data during transmission, including the precautions taken to ensure data protection. In teleradiology, transmitted as well as stored patient data have to be signed digitally according to the currently valid regulation (Roentgenverordnung, RoeV). The DICOM standard facilitates a digital signature. So far, medical image manufacturers only announced to support this security feature. We introduce a solution that extends the feature of digital signing to older modalities. (orig.)

2005-01-01

386

British Library Electronic Table of Contents (United Kingdom)

Regular physical activity (PA) decreases the risk of several chronic diseases including some cancers, type II diabetes, obesity, and cardiovascular disease; however, the majority of US adults are not meeting the recommended levels to experience these benefits. To address this public health concern, the underlying mechanisms for behavior change need to be understood, translated and disseminated into appropriately tailored interventions. The Transtheoretical Model (TTM) provides a framework for both the conceptualization and the measurement of behavior change, as well as facilitating promotion strategies that are individualized and easily adapted. The purpose of this manuscript is to present the constructs of the TTM as they relate to PA behavior change. We begin with a brief synopsis of rec...

2011-01-01

387

A PDA-Based ECG Beat Detector for Home Cardiac Care.

Tissue structure, nuclear organization and gene expression in normal and malignant breast

Recent rapid growth in mobile computing technologies enables telemedicine applications to operate on mobile devices. Our focus is on the design of an integrated electrocardiogram (ECG) beat detector on a Personal Digital Assistant (PDA) platform for the health screening process. The ECG beat detector module will be supported by the PDA version of Personal Health Information Management System (PHIMS) and Facilitated Accurate Referral Management System (FARMS) through wireless network infrastructure as a home-based mobile cardiac monitoring solution. PMID:17282192

2005-01-01

388

Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39.

Because every cell within the body has the same genetic information, a significant problem in biology is to understand how cells within a tissue express genes selectively. A sophisticated network of physical and biochemical signals converge in a highly orchestrated manner to bring about the exquisite regulation that governs gene expression in diverse tissues. Thus, the ultimate decision of a cell to proliferate, express tissue-specific genes, or apoptose must be a coordinated response to its adhesive, growth factor, and hormonal milieu. The unifying hypothesis examined in this overview is that the unit of function in higher organisms is neither the genome nor the cell alone but the complex, three-dimensional tissue. This is because there are bidirectional connections between the components of the cellular microenvironment (growth factors, hormones, and extracellular matrix) and the nucl2048 These connections are made via membrane-bound receptors and transmitted to the nucleus, where ...

2000-01-27

389

[Molecular analysis of cytoplasmic male sterility]. Progress report, June 1, 1989--June 15, 1991

We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expression of nine new and one previously reported (CcnA) sRNAs in strain D39. CcnA is one of five redundant sRNAs reported by Halfmann et al. (A. Halfmann, M. Kovacs, R. Hakenbeck, and R. Bruckner, Mol. Microbiol. 66:110-126, 2007) that are positively controlled by the CiaR response regulator. We characterized 3 of these 14 sRNAs: Spd-sr17 (144 nucleotides [nt]; decreased in stationary phase), Spd-sr37 (80 nt; strongly expressed in all growth phases), and CcnA (93 nt; induced by competence stimulatory peptide). Spd-sr17 and CcnA likely fold into structures containing single-stranded regions between hairpin structures, whereas Spd-sr37 forms a base-paired structure. Primer ...

2010-01-01

390

Vascular ATP-sensitive potassium channels are over-expressed and partially regulated by nitric oxide in experimental septic shock

We have determined the actual RNA sequence of four mitochondrial genes: pcf, nad3, rps12, and coxII. Because plant mitochondrial proteins cannot be predicted from DNA sequences, the actual primary structures of the encoded proteins were unknown. We have gained information concerning the process of RNA editing. Editing can occur before splicing. Our data is consistent with the hypothesis that RNA editing is not simultaneous with transcription. Unlike other systems, the process of editing in plant does not exhibit a discernable direction. As a result of our RNA editing studies, we have produced a number of clones of the proper coding regions tube used for incorporating these mitochondrial genes into the nucleus. 6 refs., 4 figs.

1991-12-31

391

British Library Electronic Table of Contents (United Kingdom)

Purpose To study the activation and expression of vascular (aorta and small mesenteric arteries) potassium channels during septic shock with or without modulation of the NO pathway. Methods Septic shock was induced in rats by peritonitis. Selective inhibitors of vascular KATP (PNU-37883A) or BKCa [iberiotoxin (IbTX)] channels were used to demonstrate their involvement in vascular hyporeactivity. Vascular response to phenylephrine was measured on aorta and small mesenteric arteries mounted on a wire myograph. Vascular expression of potassium channels was studied by PCR and Western blot, in the presence or absence of 1400W, an inducible NO synthase (iNOS) inhibitor. Aortic activation of the transcriptional factor nuclear factor-kappaB (NF-?B) was assessed by electrophoretic mobility shift as...

2011-01-01

392

The expression pattern of scygonadin during the ontogenesis of Scylla paramamosain predicting its potential role in reproductive immunity

British Library Electronic Table of Contents (United Kingdom)

The antimicrobial peptide scygonadin (Scy) was first isolated from the gonad of Scylla serrata and its gene is predominantly expressed in the ejaculatory duct of adult males. Thus, its function was predicted to be associated with reproductive immunity, but this is still unclear and needs further investigation. In our study, the expression pattern of Scy at different developmental stages of both male and female S. paramamosain was investigated, so that the potential function of this peptide could be examined. Using real-time quantitative PCR, Scy mRNA transcripts were demonstrated obviously in the vulnerable embryos and larvae-zoea I but very weakly detected in the larvae-zoea III, megalops and juveniles. The gene expression pattern showed a decreasing trend during the early developmental s...

2011-01-01

393

The DFNA5 gene, responsible for hearing loss and involved in cancer, encodes a novel apoptosis-inducing protein

British Library Electronic Table of Contents (United Kingdom)

DFNA5 was first identified as a gene causing autosomal dominant hearing loss (HL). Different mutations have been found, all exerting a highly specific gain-of-function effect, in which skipping of exon 8 causes the HL. Later reports revealed the involvement of the gene in different types of cancer. Epigenetic silencing of DFNA5 in a large percentage of gastric, colorectal and breast tumors and p53-dependent transcriptional activity have been reported, concluding that DFNA5 acts as a tumor suppressor gene in different frequent types of cancer. Despite these data, the molecular function of DFNA5 has not been investigated properly. Previous transfection studies with mutant DFNA5 in yeast and in mammalian cells showed a toxic effect of the mutant protein, which was not seen after transfection ...

2011-01-01

394

Taxa-specific heat shock proteins are over-expressed with crowding in the Australian plague locust

British Library Electronic Table of Contents (United Kingdom)

Most heat shock proteins (Hsps) function as molecular chaperones that help organisms to cope with stress. Although the best empirical evidence is related to heat shock, there is evidence that Hsps and their encoding genes are involved in resistance to other ecologically relevant types of stresses such as those imposed by high population density. We quantified density-dependent gene expression of large (i.e. Hsp40, Hsc70 and Hsp90) and small (Hsp20.5, Hsp20.6 and Hsp20.7) heat shock genes in neural tissue of fifth-instar nymphs of the Australian plague locust, Chortoicetes terminifera, using reverse transcription-quantitative PCR. Locusts are of particular interest when studying the influence of stress induced by high population density since they show an extreme form of phenotypic plastici...

2011-01-01

395

Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in crohn's disease

British Library Electronic Table of Contents (United Kingdom)

AbstractBackground: A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD). Methods: The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25). Results: Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) with...

2011-01-01

396

Searching for immunomodulatory sequences in sea bass (Dicentrarchus labrax L.): Transcripts analysis from thymus

British Library Electronic Table of Contents (United Kingdom)

The thymus is a key organ of the immune system in most vertebrates and, for this reason, it has been used in this paper for the generation of a normalized cDNA library from sea bass (Dicentrarchus labrax), one of the most extensively cultured species in South Mediterranean aquaculture. A total of 1632 ESTs from this library were initially analysed for sequence quality and vector sequences and, after this control, 1264 (77% of total clones sequenced) high-quality ESTs were further processed. The total collection of D. labrax thymus ESTs has been deposited in the EBI-GenBank-DBJ database (GenBank accession numbers from FN565576 to FN566839). The functional classification of ESTs was performed by Gene Ontology and KEGG annotation and, successively, the sequences were analysed using the Immuno...

2010-01-01

397

Regulation and secretion of Xanthomonas virulence factors

British Library Electronic Table of Contents (United Kingdom)

Abstract Plant pathogenic bacteria of the genus Xanthomonas cause a variety of diseases in economically important monocotyledonous and dicotyledonous crop plants worldwide. Successful infection and bacterial multiplication in the host tissue often depend on the virulence factors secreted including adhesins, polysaccharides, LPS and degradative enzymes. One of the key pathogenicity factors is the type III secretion system, which injects effector proteins into the host cell cytosol to manipulate plant cellular processes such as basal defense to the benefit of the pathogen. The coordinated expression of bacterial virulence factors is orchestrated by quorum-sensing pathways, multiple two-component systems and transcriptional regulators such as Clp, Zur, FhrR, HrpX and HpaR. Furthermore, virule...

2010-01-01

398

Referential Failures and Affective Reactivity of Language in Schizophrenia and Unipolar Depression

British Library Electronic Table of Contents (United Kingdom)

Reference failures, and their increase in affectively negative conditions (known as affective reactivity of speech), are more frequently observed in schizophrenia patients than in normal controls, but no information is available comparing schizophrenia with depression, ie, a mental disorder closely linked to the concept of affective reactivity. To address this gap in the literature, the present study compared 24 schizophrenia inpatients, 21 unipolar depression inpatients and 21 normal controls. Two 10-minute conversational speech samples (1 on negative and 1 on positive memories) were collected from each patient. The transcripts of the audiotaped interviews were analyzed blindly for frequencies of 6 types of referential failures, employing the Communication Disturbances Index, adapted for ...

2011-01-01

399

Quercetin-induced downregulation of phospholipase D1 inhibits proliferation and invasion in U87 glioma cells

British Library Electronic Table of Contents (United Kingdom)

Phospholipase D (PLD) has been recognized as a regulator of cell proliferation and tumorigenesis, but little is known about the molecules regulating PLD expression. Thus, the identification of small molecules inhibiting PLD expression would be an important advance in PLD-mediated physiology. Quercetin, a ubiquitous bioactive flavonoid, is known to inhibit proliferation and induce apoptosis in a variety of cancer cells. In the present study, we examined the effect of quercetin on the expression of PLD in U87 glioma cells. Quercetin significantly suppressed the expression of PLD1 at the transcriptional level. Moreover, quercetin abolished the protein expression of PLD1 in a time and dose-dependent manner, as well as inhibited PLD activity. Quercetin suppressed NFkB-induced PLD1 expression vi...

2011-01-01

400

Purpurin is a key molecule for cell differentiation during the early development of zebrafish retina

British Library Electronic Table of Contents (United Kingdom)

Recently, we cloned purpurin cDNA as an upregulated gene in the axotomized fish retina. The retina-specific protein was secreted from photoreceptors to ganglion cell layer during an early stage of optic nerve regeneration in zebrafish retina. The purpurin worked as a trigger molecule for axonal regrowth in adult injured fish retina. During zebrafish development, purpurin mRNA first appeared in ventral retina at 2 days post-fertilization (dpf) and spread out to the outer nuclear layer at 3 dpf. Here, we investigated the role of purpurin for zebrafish retinal development using morpholino gene knockdown technique. Injection of purpurin morpholino into the 1-2 cell stage of embryos significantly inhibited the transcriptional and translational expression of purpurin at 3 dpf. In the purpurin mo...

2009-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Protein expression following heat shock in the nervous system of Locusta migratoria

British Library Electronic Table of Contents (United Kingdom)

There is a thermal range for the operation of neural circuits beyond which nervous system function is compromised. Locusta migratoria is native to the semiarid regions of the world and provides an excellent model for studying neural phenomena. In this organism previous exposure to sublethal high temperatures (heat shock, HS) can protect neuronal function against future hyperthermia but, unlike many organisms, the profound physiological adaptations are not accompanied by a robust increase of Hsp70 transcript or protein in the nervous system. We compared Hsp70 increase following HS in the tissues of isolated and gregarious locusts to investigate the effect of population density. We also localized Hsp70 in the metathoracic ganglion (MTG) of gregarious locusts to determine if HS affects Hsp70 ...

2011-01-01

402

Probiotic-Induced Priming of Innate Immunity to Protect Against Rotaviral Infection

British Library Electronic Table of Contents (United Kingdom)

Understanding of probiotic-induced regulatory gene expression and networking is critical to further explore their roles in controlling infection. Transcriptional profile of selected innate immune genes in primary bovine intestinal epithelial cells was assessed over a time course of incubation with the probiotic Lactobacillus plantarum 299v. Based on gene expression results, a time point was chosen to prime epithelial cells with the probiotic prior to infection with rotavirus. Plaque assays and genomic analysis provided the basis for establishing the efficacy of probiotics in preventing a rotaviral infection. Plaque assays revealed that the probiotic is capable of decreasing (at least by 100-fold) the levels of live virus when the cells were primed with the probiotic. Results from gene expr...

2010-01-01

403

Overexpression of MAD2 predicts clinical outcome in primary lung cancer patients

British Library Electronic Table of Contents (United Kingdom)

High-level expression of mitotic arrest defective protein 2 (MAD2), a central component of the spindle assembly checkpoint, has been observed in a variety of human malignancies. Aim of the present study was to observe the expression of MAD2 in human non-small-cell lung cancer (NSCLC) and explore its clinicopathologic significance and evaluate MAD2 expression as a prognostic marker. MAD2 transcript was found to be overexpressed in the great majority of lung cancers by semi-quantitative RT-PCR. A total of 358 NSCLCs were analyzed immunohistochemically on tissue microarrays. High-level MAD2 expression was observed in 26.3% (94 of 358 cases), and correlated with male sex (P=0.0002), tumor progression (pT status) (P=0.0009), visceral or parietal pleural invasion (P=0.0151), non-adenocarcinoma, ...

2011-01-01

404

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase

British Library Electronic Table of Contents (United Kingdom)

We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step PCR pathogen detection using established real-time detection methods. We demonstrate that this Taq polymerase mutant (Taq M1) has similar PCR sensitivity and nuclease activity as the respective Taq wild-type DNA polymerase. In addition, and in marked contrast to the wild-type, Taq M1 exhibits a significantly increased reverse transcriptase activity especially at high temperatures (>60degreeC). RNA generally hosts highly stable secondary structure motifs, such as hairpins and G-quadruplexes, which complicate, or in the worst case obviate, reverse transcription (RT). Thus, RT at hi...

2010-01-01

405

Morphologie des cellules de levure et la reproduction sexuelle - Apercu general et quelques considerations

British Library Electronic Table of Contents (United Kingdom)

Over the decades, basic research in life sciences has profited greatly from the study of the small unicellular fungal species Saccharomyces cerevisiae. This yeast turned out to be key for the identification and understanding of molecular mechanisms that underlay the basic functions of all eukaryotic cells. These include, but are not limited to, the regulatory mechanisms behind cellular reproduction (cell cycle control), cellular morphogenesis (cell polarity, cytoskeleton and membrane trafficking) and the management of cellular information (chromosome biology, transcription and translation). Rapid access to genomic information of many yeast species, combined with bioinformatics analyses, provide information on the evolutionary history of yeasts and the molecular ancestry of their constituen...

2011-01-01

406

Molecular environment of the IIId subdomain of the IRES element of hepatitits C virus RNA on the human 40S ribosomal subunit

British Library Electronic Table of Contents (United Kingdom)

The molecular environment of the key subdomain IIId of the internal ribosome entry site (IRES) element of hepatitis C virus (HCV) RNA in the binary complex with the human 40S ribosomal subunit was studied. To this end, HCV IRES derivatives bearing perfluorophenylazido groups activatable by mild UV at nucleotides G263 or A275 in the subdomain IIId stem were used. They were prepared by the complementary addressed modification of the corresponding RNA transcript with alkylating oligodeoxyribonucleotide derivatives. None of the RNA derivatives were shown to be crosslinked to the 18S rRNA. It was found that the photoreactive groups of the IRES G263 and A275 nucleotides are crosslinked to ribosomal proteins S3a, S14, and S16. For the IRES derivative with the photoreactive group in nucleotide G26...

2009-01-01

407

Long-distance transport, vacuolar sequestration, tolerance, and transcriptional responses induced by cadmium and arsenic

British Library Electronic Table of Contents (United Kingdom)

Iron, zinc, copper and manganese are essential metals for cellular enzyme functions while cadmium, mercury and the metalloid arsenic lack any biological function. Both, essential metals, at high concentrations, and non-essential metals and metalloids are extremely reactive and toxic. Therefore, plants have acquired specialized mechanisms to sense, transport and maintain essential metals within physiological concentrations and to detoxify non-essential metals and metalloids. This review focuses on the recent identification of transporters that sequester cadmium and arsenic in vacuoles and the mechanisms mediating the partitioning of these metal(loid)s between roots and shoots. We further discuss recent models of phloem-mediated long-distance transport, seed accumulation of Cd and As and rec...

2011-01-01

408

Epigenetic regulation of autosomal gene expression by sex chromosomes

British Library Electronic Table of Contents (United Kingdom)

Males and females display differences in physiology, behaviour and susceptibility to many diseases. Genome-wide transcription profiling studies have uncovered large-scale sex differences in autosomal gene expression in somatic tissues that are thought to underlie such 'sexual dimorphisms'. Because males and females differ genetically mainly in their sex chromosome complement, most sex differences can be traced back to the X and Y chromosomes. Although sex hormones are usually considered the main architects of sexual dimorphisms, recent studies have demonstrated that sex chromosomes can also induce sex differences in somatic gene expression in the absence of hormonal differences. The recent discovery of epigenetic sex differences that are not hormone-induced brings us closer to understandin...

2011-01-01

409

Distinctive Responses of Metabolically Active Microbiota to Acidification in a Thermophilic Anaerobic Digester

British Library Electronic Table of Contents (United Kingdom)

Acidification is one of the most common and serious problems inducing process failure in anaerobic digesters. The production of volatile fatty acids (VFAs) mainly triggers acidic shock. However, little is known about the bacteria involved in the processes of acidogenic metabolism, such as fermentation and reductive acetogenesis. Here, the metabolic responses of a methanogenic community to the acidification and resulting process deterioration were investigated using transcriptional profiling of both the 16S rRNA and formyltetrahydrofolate synthetase (FTHFS) genes. The 16S rRNA-based analyses demonstrated that the dynamic shift of bacterial populations was closely correlated with reactor performance, especially with VFA accumulation levels. The pH drop accompanied by an increase in VFAs stim...

2011-01-01

410

Crop diversification and trade liberalization: Linking global trade and local management through a regional case study

British Library Electronic Table of Contents (United Kingdom)

Some models anticipate that liberalized agricultural trade will lead to increased crop diversity, while other models make the opposite claim. These positions were explored in southwestern British Columbia, Canada where, between 1992 and 1998, government subsidies and other measures designed to protect horticultural farmers were lifted, exposing these farmers to foreign competition. Public hearings on the future of agriculture provided an opportunity to tap the knowledge and experience of people affected by this transition. Analysis of transcripts from these hearings, which was confirmed by industry data, shows that trade liberalization has led to the loss of the local fruit and vegetable processing industry. Stakeholders saw the loss as a major factor affecting the choice of crops grown lo...

2006-01-01

411

Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing

British Library Electronic Table of Contents (United Kingdom)

To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-...

2011-01-01

412

Cell fate regulation by coupling mechanical cycles to biochemical signaling pathways

British Library Electronic Table of Contents (United Kingdom)

Many aspects of cellular motility and mechanics are cyclic in nature such as the extension and retraction of lamellipodia or filopodia. Inherent to the cycles of extension and retraction that test the environment is the production of mechano-chemical signals that can alter long-term cell behavior, transcription patterns, and cell fate. We are just starting to define such cycles in several aspects of cell motility, including periodic contractions, integrin cycles of binding and release as well as the normal oscillations in motile activity. Cycles of local cell contraction and release are directly coupled to cycles of stressing and releasing extracellular contacts (matrix or cells) as well as cytoplasmic mechanotransducers. Stretching can alter external physical properties or sites exposed b...

2009-01-01

413

Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells

Potent, selective and cell penetrant inhibitors of SF-1 by functional ultra-high-throughput screening.

Two candidate small interfering RNAs (siRNAs) corresponding to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike gene were designed and in vitro transcribed to explore the possibility of silencing SARS-CoV S gene. The plasmid pEGFP-optS, which contains the codon-optimized SARS-CoV S gene and expresses spike-EGFP fusion protein (S-EGFP) as silencing target and expressing reporter, was transfected with siRNAs into HEK 293T cells. At various time points of posttransfection, the levels of S-EGFP expression and amounts of spike mRNA transcript were detected by fluorescence microscopy, flow cytometry, Western blot, and real-time quantitative PCR, respectively. The results showed that the cells transfected with pEGFP-optS expressed S-EGFP fusion protein at a higher level compared with those transfected with pEGFP-S, which contains wildtype SARS-CoV spike gene sequence. The green fluorescence, mean fluorescence intensity, and SARS-CoV S RNA ...

2004-11-26

414

Increased expression after x-irradiation of MUC1 in cultured human colon carcinoma HT-29 cells

The steroidogenic factor 1 (SF-1, also known as NR5A1) is a transcription factor belonging to the nuclear receptor superfamily. Whereas most of the members of this family have been extensively characterized, the therapeutic potential and pharmacology of SF-1 still remains elusive. Described here is the identification and characterization of selective inhibitory chemical probes of SF-1 by a rational ultra-high-throughput screening (uHTS) strategy. A set of 64,908 compounds from the National Institute of Health's Molecular Libraries Small Molecule Repository was screened in a transactivation cell-based assay employing a chimeric SF-1 construct. Two analogous isoquinolinones, ethyl 2-[2-[2-(2,3-dihydro-1,4-benzodioxin-7-ylamino)-2-oxoethyl]-1-oxoisoquinolin-5-yl]oxypropanoate (SID7969543) and ethyl 2-[2-[2-(1,3-benzodioxol-5-ylmethylamino)-2-oxoethyl]-1-oxoisoquinolin-5-yl]oxypropanoate and (SID7970631), were identified as potent submicromolar inhibitors, yielding ...

2008-03-11

415

Expression of bacterial luciferase in eukaryotic cells

The effect of X-irradiation on production of MUC1 was studied with human colon carcinoma HT-29 cells. As evaluated by immunocytochemical staining, the percentages or MUC1-positive cells in cells at 4 days after 6 Gy irradiation and in unirradiated control cells were 52#+-#3.5% (n=6) and 26#+-#2.8% (n=6), respectively. Flow-cytometric analysis of living cells showed that MUC1 began to rise from day 1, reaching a plateau by day 4 after 6 Gy irradiation. Western blot analysis with monoclonal antibody MY.1E12 against glycosylated MUC1 (mature form) showed dose-dependent increases of two bands (500 and 390 kDa) corresponding to two polymorphic MUC1 alleles. Premature forms of MUC1 (350 and 240 kDa) were detectable with monoclonal antibody HMFG-2 only in irradiated cells, suggesting that new core protein synthesis had been induced. The transcriptional activity of the MUC1 gene was analyzed in terms of transient expression of MUC1-CAT reporter plasmids containing ...

2000-03-01

416

Chemopreventive effects of Furan-2-yl-3-pyridin-2-yl-propenone against 7,12-dimethylbenz[a]anthracene-inducible genotoxicity

Expression of Bacterial luciferase enzyme (lux) in mammalian cells would be a powerful bioreporter protein system for in vivo imaging because eukaryotic luciferases need expensive substrates. However, only a few efforts have been made to express bacterial luciferase enzyme in mammalian cells. As the result of this, we attempted to construct bicistronic vector including two bacterial luciferase genes (LuxA and LuxB) for assessing the potential to be visualized in vitro or in vivo by optical imaging system after transfection to mammalian cells. We designed and synthesized luxA and luxB genes from Photorhabdus Luminescens. To co-express both luxA and luxB genes from a single promoter, we cloned as a bicistronic transcript fused with an internal ribosomal entry site (IRES). This bicistronic transcript was transfected by Superfect to HEK 293T cell line. We also transfected lux A and lux B vector to HEK 293T cells separately. To evaluate gene ...

2005-11-18

417

Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution

1-Furan-2-yl-3-pyridin-2-yl-propenone (FPP-3) is an anti-inflammatory agent with a propenone moiety and chemically synthesized recently. In this study, we examined the chemopreventive effect of FPP-3 on 7,12-dimethylbenz[a]anthracene (DMBA)-induced genotoxicity in MCF-7 cells. FPP-3 reduced the formation of the DMBA-DNA adduct. DMBA-induced CYP1A1 and CYP1B1 gene expression and enzyme activity were inhibited by FPP-3. It inhibited DMBA-induced aryl hydrocarbon receptor (AhR) transactivation and DMBA-inducible nuclear localization of the AhR. Induction of detoxifying phase II genes by chemopreventive agents represents a coordinated protective response against oxidative stress and neoplastic effects of carcinogens. Transcription factor NF-E2 related factor 2 (Nrf2) regulates antioxidant response element (ARE) of phase II detoxifying and antioxidant enzymes, such as glutathione S-transferase (GST) and NAD(P)H:quinone oxidoreductase (QR). FPP-3 increased the expression ...

2008-05-01

418

Well-test data from geothermal reservoirs

The DNA sequence motif ATTTGCAT (octamer) or its inverse complement has been identified as an evolutionarily conserved element in the promoter region of immunoglobulin genes. Two major DNA-binding proteins that bind in a sequence-specific manner to the octamer DNA sequence have been identified in mammalian species--a ubiquitously expressed protein (Oct-1) and a lymphoid-specific protein (Oct-2). During characterization of the promoter region of the chicken immunoglobulin light chain gene, the authors identified two homologous octamer-binding proteins in chicken B cells. when the cloning of the human gene for Oct-2 revealed it to be a member of a distinct family of homeobox genes, they sought to determine if the human Oct-2 cDNA could be used to identify homologous chicken homeobox genes. Using a human Oct-2 homeobox-specific DNA probe, they were able to identify 6-10 homeobox-containing genes in the chicken genome, demonstrating that the Oct-2-related subfamily of homeobox genes exists ...

1990-02-01

419

Using value engineering to facilitate PWAs

Extensive well testing in geothermal resources has been carried out throughout the western United States and in northern Mexico since 1975. Each resource tested and each well test conducted by LBL during the eight-year period are covered in brief. The information, collected from published reports and memoranda, includes test particulars, special instrumentation, data interpretation when available, and plots of actual data. Brief geologic and hydrologic descriptions of the geothermal resources are also presented. The format is such that well test descriptions are grouped, in the order performed, into major sections according to resource, each section containing a short resource description followed by individual test details. Additional information regarding instrumentation is provided. Source documentation is provided throughout to facilitate access to further information and raw data.

1982-09-01

420

Unravelling the complexity of collective mental models: A method for developing and analysing scenarios in multi-organisational contexts

Value Engineering (VE) is a problem solving methodology that has been used in manufacturing and construction industries for fifty years to improve products, systems and projects while reducing unnecessary cost. A Process Waste Assessment (PWA) is a newly developed methodology designed to characterize waste streams and identify opportunities to reduce or eliminate waste generation. The VE and PWA methodologies are compared to show their general similarities and specific differences, and to suggest how VE can be woven into the PWA methodology. Further, the roles of the VE and PWA team leaders and their training are compared; suggestions are made to help enable the PWA team leader to more effectively lead a group-centered creative process. Examples of how VE has been used in hazardous and radioactive waste minimization and pollution prevention projects are presented, also.

1993-03-19

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421

British Library Electronic Table of Contents (United Kingdom)

In multi-organisational contexts, scenario building has been used to engage stakeholders in a critical discussion on issues of mutual importance, and to gain their support with regards to possible future responses. A review of existing literature suggests that much has been written regarding the process of scenario development and the benefits of the process, but the detailed analysis of scenario building outcomes, which encompass a large number of issues and their complex interconnections, has not been made explicit for studying and enhancing understanding of a complex societal problem. This paper presents a systematic method for analysing such complex outcomes in order to facilitate reflective thinking on important issues within the wider context for policy development. The method was em...

2011-01-01

422

Trials and tribulations of an African-led research and capacity development programme: the case for EDCTP investments

British Library Electronic Table of Contents (United Kingdom)

Summary We describe the initiation and establishment of The University of Zambia - University College London Medical School (UNZA-UCLMS) Research and Training Project, an entirely African scientist-led, south-north partnership. In its 16 year existence, the project, by successfully obtaining competitive grant funding, has transformed itself into one of Africa's most productive African-led R&D programmes with training and visible research outputs. The project serves as a role model and now networks R&D and training activities with six southern African (10 institutions) and six European countries. This project case study illustrates that deep commitment is essential for success and that the factors which facilitate success in R&D in Africa need to be evaluated. The long-term prospects for su...

2010-01-01

423

Training And Education Needs In Radiological Protection - First Results Of The ENETRAP Survey

The mechanisms of ethene and propene formation from methanol over high silica H-ZSM-5 and H-beta

Recent studies have shown that there is a wide variety of approaches to education and training of the Qualified Expert across the European Union. National education and training programmes show often large differences in content, duration, level, the introduction of practical work, etc. As they stand, such differences are a barrier to the mutual recognition of the Qualified Expert status and, in part, are contributing to a perceived shortage in expertise in radiation protection and safety. The overall aim of ENETRAP is to determine mechanisms that in the longer term will facilitate better integration of education and training activities (with a view to mutual recognition across the European Union) and to ensure the ongoing provision of the necessary competence and expertise at the level of the Qualified Expert. The ENETRAP project is a 6FP coordination action. It started in April 2005 and runs over a period of 24 months. (authors)

2006-07-01

424

British Library Electronic Table of Contents (United Kingdom)

Selectivity control is a key issue in the zeolite facilitated conversion of methanol to hydrocarbons. This work addresses: (a) How the zeolite topology (MFI versus BEA) determines the type of intermediate participating in the catalytic cycle thereby controlling the ethene/propene product selectivity in the methanol to alkenes reaction, and (b) to what extent light alkene formation occurs via aromatic and alkene reaction intermediates for H-ZSM-5. Three catalyst samples have been studied in experiments relying on isotopic labeling. For H-beta, penta- and hexamethylbenzene are involved in an aromatics based hydrocarbon pool type mechanism leading predominantly to propene and the higher alkenes (a very low yield of ethene is observed), whereas for H-ZSM-5, the lower methylbenzenes are the aro...

2009-01-01

425

The geometry of lie algebras and broken SO(6) symmetries

CERN Document Server

Non-linear realisations of the groups SU(2), SO(1,4) and SO(2,4) are analysed, described by the coset spaces SU(2)/U(1), SO(1,4)/SO(1,3) and SO(2,4)/SO(1,3) x SO(1,1). The Lie algebras of certain special unitary and special orthogonal groups are studied and their projection operators are determined in order to facilitate the above analyses, in particular that of SO(2,4)/SO(l,3) x SO(1,1). The analysis consists of determining the transformation properties of the Goldstone bosons, constructing the most general possible Lagrangian for the realisations and finding the metric of the coset space.

2001-01-01

426

The effect of temperature on the grain growth of nanocrystalline metals and its simulation by molecular dynamics method

British Library Electronic Table of Contents (United Kingdom)

In this study, the thermodynamic stability of the grain boundaries and the grain growth of nanocrystalline Palladium (Pd) at various temperatures were investigated. For this purpose, the Gibbs free energy curves of grain boundaries were plotted in terms of the excess volume by the use of the equation of state (EOS) and Song's thermodynamic models. The results showed that, according to the prediction of these models, the nanocrystalline growth in metals was stopped at the grain sizes less than the critical grain size. Also, the results of the temperature variations and its effect on the Gibbs free energy curves showed that by the increase of the temperature, the possibility for the stoppage of grain growth is facilitated and the critical grain size is increased. To investigate the validity ...

2012-01-01

427

The disruption of l-carnitine metabolism by aluminum toxicity and oxidative stress promotes dyslipidemia in human astrocytic and hepatic cells

British Library Electronic Table of Contents (United Kingdom)

l-Carnitine is a critical metabolite indispensable for the metabolism of lipids as it facilitates fatty acid transport into the mitochondrion where b-oxidation occurs. Human astrocytes (CCF-STTG1 cells) and hepatocytes (HepG2 cells) exposed to aluminum (Al) and hydrogen peroxide (H2O2), were characterized with lower levels of l-carnitine, diminished b-oxidation, and increased lipid accumulation compared to the controls. g-Butyrobetainealdehyde dehydrogenase (BADH) and butyrobetaine dioxygenase (BBDOX), two key enzymes mediating the biogenesis of l-carnitine, were sharply reduced during Al and H2O2 challenge. Exposure of the Al and H2O2-treated cells to a-ketoglutarate (KG), led to the recovery of l-carnitine production with the concomitant reduction in ROS levels. It appears that the chann...

2011-01-01

428

The composition-explicit distillation curve technique: Relating chemical analysis and physical properties of complex fluids

British Library Electronic Table of Contents (United Kingdom)

The analysis of complex fluids such as crude oils, fuels, vegetable oils and mixed waste streams poses significant challenges arising primarily from the multiplicity of components, the different properties of the components (polarity, polarizability, etc.) and matrix properties. We have recently introduced an analytical strategy that simplifies many of these analyses, and provides the added potential of linking compositional information with physical property information. This aspect can be used to facilitate equation of state development for the complex fluids. In addition to chemical characterization, the approach provides the ability to calculate thermodynamic properties for such complex heterogeneous streams. The technique is based on the advanced distillation curve (ADC) metrology, wh...

2010-01-01

429

The Lithium future-resources, recycling, and the environment

British Library Electronic Table of Contents (United Kingdom)

Abstract:- The demand for Lithium-ion batteries as a major power source in portable electronic devices and vehicles is rapidly increasing. I use cumulative data of vehicle, mobile phone, laptop, and digital camera production to show that demand will overshoot the available global Lithium resources before 2025. Even if 100% of all Lithium-ion batteries were recycled today, recycling could not prevent this resource depletion in time. As the increasing Lithium scarcity will increase the price, it will be feasible to mine diluted resources with a strong environmental impact. I highlight these impacts in Lithium-rich Bolivia, the potential new -Saudi Arabia of Lithium.- Lithium extraction is likely to cause substantial water pollution, and-through impacts on native diversity-facilitate human he...

2011-01-01

430

Synthesis of C-11 iodoantipyrine for positron emission tomography

Synthesis and study of the antifungal activity of new mono- and disubstituted derivatives of a genetically engineered polyene antibiotic 28,29-didehydronystatin A1 (S44HP)

We have developed a method for the synthesis of C-11 iodoantipyrine. Carbon-11-labeled methyl iodide, prepared from /sup 11/CO2, was used to methylate 3-methyl-1-phenyl-2-pyrazolin-5-one to form C-11 antipyrine. Following silica-gel column chromatography and iodination, radiochemical purity of the C-11 iodoantipyrine was more than 99.5%, with a 10% yield and a specific activity of 30 mCi/mumol. Preliminary animal studies showed complete cerebral extraction and local cerebral blood-flow values that were within 4.6% of those obtained using C-14 iodoantipyrine. The C-11 analog, with positron emission tomography, will facilitate local cerebral blood-flow studies in human subjects.

1981-06-01

431

British Library Electronic Table of Contents (United Kingdom)

Mono- and disubstituted novel derivatives of the heptaene nystatin analog 28,29-didehydronystatin A1 (S44HP, 1) were obtained by chemical modification of the exocyclic C-16 carboxyl and/or an amino group of mycosamine moiety. The strategy of preparation of mono- and double-modified polyene macrolides was based on the use of intermediate hydrophobic N-Fmoc (9-fluorenylmethoxycarbonyl) derivatives that facilitated the procedures of isolation and purification of new compounds. The antifungal activity of the new derivatives was first tested in vitro against yeasts and filamentous fungi, allowing the selection of the most active compounds that were subsequently tested for acute toxicity in mice. 2-(N,N-dimethylamino)ethylamide of 1 (2) and 2-(N,N-dimethylamino)ethylamide of N-fructopyranosyl-28...

2010-01-01

432

Study of coastal water zone ecosystem health in Zhejiang Province based on remote sensing data and GIS

British Library Electronic Table of Contents (United Kingdom)

The coastal ecosystem health assessment is a field of increasing importance. In this paper, a preliminary assessment of ecosystem health in Zhejiang coastal water zone was made, mainly based on remote sensing data and GIS technique. Its spatial and quantitative evaluation was facilitated by the progress of remote sensing and GIS technique development. Firstly, human activities, hydrology and ecosystem problems in the study area were discussed and analyzed. Secondly, from 4 aspects of human stress, physical, chemical and biological responses to anthropogenic activities and natural stress, several indicators such as water transparency (Secchi Disk Depth, SDD), suspended substance concentration, dissolved inorganic nitrogen, active phosphate, chlorophyll, harmful algae bloom, as well as distr...

2010-01-01

433

Studies of the reduction mechanism of selenium dioxide and its impact on the microstructure of manganese electrodeposit

British Library Electronic Table of Contents (United Kingdom)

The influence of selenium dioxide (SeO2) on the microstructure and electrodeposition of manganese coatings obtained from a sulfate based neutral solution was investigated by material characterization methods and electrochemical techniques. The crystal structure and surface morphology of these coatings were studied by scanning electron microscopy (SEM) and powder X-ray diffraction spectroscopy (XRD), respectively. The SEM and XRD data showed that SeO2 could effectively accelerate phase transformation, and facilitate leveled and fine grain growth. The electrochemical results indicated that SeO2 could inhibit hydrogen evolution reaction and promote manganese deposition. The action of selenium dioxide in manganese deposition was found to be a reduction and adsorption mechanism. The process cou...

2011-01-01

434

Strategies and computational tools for improving randomized protein libraries.

Spatio-temporal overlap between Yellowstone bison and elk - implications of wolf restoration and other factors for brucellosis transmission risk

In the last decade, directed evolution has become a routine approach for engineering proteins with novel or altered properties. Concurrently, a trend away from purely 'blind' randomization strategies and towards more 'semi-rational' approaches has also become apparent. In this review, we discuss ways in which structural information and predictive computational tools are playing an increasingly important role in guiding the design of randomized libraries: web servers such as ConSurf-HSSP and SCHEMA allow the prediction of sites to target for producing functional variants, while algorithms such as GLUE, PEDEL and DRIVeR are useful for estimating library completeness and diversity. In addition, we review recent methodological developments that facilitate the construction of unbiased libraries, which are inherently more diverse than biased libraries and therefore more likely to yield improved variants. PMID:16095966

2005-10-01

435

British Library Electronic Table of Contents (United Kingdom)

Summary 1. In the Greater Yellowstone Ecosystem, bison Bison bison and elk Cervus canadensis nelsoni act as hosts for Brucella abortus. The presence of B. abortus within wildlife populations is an important conservation issue because of the risk of brucellosis transmission from wildlife to cattle. 2. We investigated conditions facilitating contact between bison (40-60% seroprevalence) and elk on a shared winter range in the Madison headwaters area of Yellowstone National Park. We evaluated the effects of snow pack, season, elk and bison population sizes, and wolf Canis lupus predation risk on the degree of spatial overlap between bison and elk from 1991 to 2006. 3. Sixty-eight per cent of 10 093 independent elk observations occurred within the known bison wintering range, 29% occurred with...

2010-01-01

436

Some nuclear data needs in astrophysics

Some geotechnical aspects of the Hyogo-ken Nanbu (Kobe) earthquake of January 17, 1995

In this paper we discuss a number of astrophysical environments and how improved nuclear data could facilitate a better understanding of them. One area of interest includes proton and alpha-particle reactions with unstable nuclei which are necessary for understanding the nucleosynthesis and energy generation in hot hydrogen-burning environments. Efforts underway at LLNL and elsewhere to develop the technology for the measurement of these reaction rates are discussed. Heavy-element nucleosynthesis in the late stages of red-giant stars and supernovae requires a complete network of neutron capture rates and beta-decay rates for nuclei near and far from stability. Experimental and theoretical efforts at LLNL to supply the input data and to model the nucleosynthetic environments will be outlined. Suggestions are made as to which nuclear data are most critical for the various scenarios. 42 refs., 11 figs., 1 tab.

1985-05-01

437

Solar energy conversion system

A geological and geotechnical framework was provided in order to facilitate understanding of the damage to structures resulting from soil conditions during the earthquake of January 17, 1995 at Hyogo-ken Nanbu (Kobe), Japan. Special attention was paid to the large scale failures of the quay walls in the port of Kobe, resulting from liquefaction. The performance of structures in improved and unimproved ground were compared. It was observed that locations with some previous site improvements through densification behaved satisfactorily, despite unanticipated high levels of ground motion. Soil-structure interaction problems, such as pile foundations, bridge piers, lifelines, and an underground subway station were also described. Reference was made to the significance of these failures for the seismic design in the Fraser Delta in British Columbia in view of the area`s proneness to liquefaction during a major earthquake. 31 refs., 5 tabs., 45 figs.

1996-06-01

438

Software development for a switch-based data acquisition system

A solar energy collection and conversion system is disclosed in which a cassegrain mirror system is rotated about a diurnal axis, which axis is adjusted for seasonal variations in the incidence of the sun's rays on the earth's surface. A black body absorption cavity filled with translucent or transparent fluid material is used for direct absorption of the sun's rays. The incident solar energy is absorbed directly by the fluid medium. The fluid within the cavity may be maintained under extremely high pressures and temperatures in order to utilize this fluid as a heat storage medium. Such heat storage is facilitated by apparatus which permits a change in the volume of the reflective cavity in response to the temperature or pressure of the fluid within the cavity.

1981-09-01

439

Software development for a switch-based data acquisition system

We report on the software aspects of the development of a switch-based data acquisition system at Fermilab. This paper describes how, with the goal of providing an integrated systems engineering'' environment, several powerful software tools were put in place to facilitate extensive exploration of all aspects of the design. These tools include a simulation package, graphics package and an Expert System shell which have been integrated to provide an environment which encourages the close interaction of hardware and software engineers. This paper includes a description of the simulation, user interface, embedded software, remote procedure calls, and diagnostic software which together have enabled us to provide real-time control and monitoring of a working prototype switch-based data acquisition (DAQ) system.

1991-12-01

440

Servicing a world elsewhere? Examining everyday work practices in the emerging economic spaces of Malaysia's business process outsourcing industry

We report on the software aspects of the development of a switch-based data acquisition system at Fermilab. This paper describes how, with the goal of providing an ``integrated systems engineering`` environment, several powerful software tools were put in place to facilitate extensive exploration of all aspects of the design. These tools include a simulation package, graphics package and an Expert System shell which have been integrated to provide an environment which encourages the close interaction of hardware and software engineers. This paper includes a description of the simulation, user interface, embedded software, remote procedure calls, and diagnostic software which together have enabled us to provide real-time control and monitoring of a working prototype switch-based data acquisition (DAQ) system.

1991-12-01

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441

British Library Electronic Table of Contents (United Kingdom)

The paper seeks to illuminate everyday work practices in the economic spaces of Malaysia's business process outsourcing (BPO) industry. It draws upon empirical evidence from call centre staff and promotes analytical sensitivity to the experience of labour and spatiotemporal relations within the offshore back offices of multinational corporations located in Cyberjaya, Malaysia. The analysis provides an empirical snapshot of how IT-facilitated work practices in the global information economy are embedded and socially reproduced in everyday settings. To this end, the paper engages anthropologically with BPO labour, and critiques -globalist- representations of economic and social transformation frequently articulated through an abstract, dehumanized space of networks and flows often seen to wa...

2011-01-01

442

Sequences of versatile, broad-host-range vectors of the RK2 family.

Sea-level rise and coastal zone management

Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available.

2003-07-01

443

Removal of short range order minerals prior to grain size analysis of volcanic ash soils

Greenhouse-effect induced climate change repercussions will, via the catchment areas of big rivers, eventually arrive in amplified form at the far downstream coastal zones of the world. In the coastal zone these imported problems will add to the locally exerted climate change effects, while the climate change induced sea-level rise will attack the coastal zone from the other side, both by flooding and by saline groundwater intrusion. These multiple problems will hit the coastal zone, which is already under increasing stress. The combined threats require fast and massive support for national Coastal Zone Management capabilities, which could help to limit the damage and to support planning for sustainable development. Recommendations are presented to facilitate implementation of Coastal Zone Management units in all coastal countries by the year 2000.

1992-09-01

444

British Library Electronic Table of Contents (United Kingdom)

Abstract Incomplete dispersion of aggregates is a well known difficulty when analyzing soil texture of Andosols, particularly if no fresh soil material is available. To facilitate such investigations, several dispersion procedures were carried out on air dried samples rich in short range order minerals, originating from selected Andosol profiles (S Mexico). As a result, we propose an improved method based on a sequential chemical treatment with K oxalate, NH4 oxalate, and oxalic acid that reliably removes cementing agents and allows for a stable dispersion of soil particles after addition of Na pyrophosphate. This method was proved to be well reproducible and highly reliable for routine grain size determinations. The potential error caused by the inherent risk of dissolving primary mineral...

2010-01-01

445

Pinellas Plant Environmental Baseline Report

Phenotypic plasticity in sex allocation for a simultaneously hermaphroditic coral reef fish

The Pinellas Plant has been part of the Department of Energy`s (DOE) nuclear weapons complex since the plant opened in 1957. In March 1995, the DOE sold the Pinellas Plant to the Pinellas County Industry Council (PCIC). DOE has leased back a large portion of the plant site to facilitate transition to alternate use and safe shutdown. The current mission is to achieve a safe transition of the facility from defense production and prepare the site for alternative uses as a community resource for economic development. Toward that effort, the Pinellas Plant Environmental Baseline Report (EBR) discusses the current and past environmental conditions of the plant site. Information for the EBR is obtained from plant records. Historical process and chemical usage information for each area is reviewed during area characterizations.

1997-06-01

446

British Library Electronic Table of Contents (United Kingdom)

Phenotypic plasticity can facilitate reproductive strategies that maximize mating success in variable environments and lead to differences in sex allocation among populations. For simultaneous hermaphrodites with sperm competition, including Serranus tortugarum a small coral reef fish, proportional male allocation (testis in total gonad) is often greater where local density or mating group size is higher. We tested whether S. tortugarum reduced male allocation when transplanted from a higher density site to a lower density site. After 4?months, transplants mirrored the sex-allocation patterns of the resident population on their new reef. Transplants had significantly lower male allocation than representatives from their source population, largely as a result of reduced testis mass relative...

2011-01-01

447

Performance-Aware Power Management in Embedded Controllers with Multiple-Voltage Processors

CERN Document Server

The goal of this work is to minimize the energy dissipation of embedded controllers without jeopardizing the quality of control (QoC). Taking advantage of the dynamic voltage scaling (DVS) technology, this paper develops a performance-aware power management scheme for embedded controllers with processors that allow multiple voltage levels. The periods of control tasks are adapted online with respect to the current QoC, thus facilitating additional energy reduction over standard DVS. To avoid the waste of CPU resources as a result of the discrete voltage levels, a resource reclaiming mechanism is employed to maximize the CPU utilization and also to improve the QoC. Simulations are conducted to evaluate the performance of the proposed scheme. Compared with the optimal standard DVS scheme, the proposed scheme is shown to be able to save remarkably more energy while maintaining comparable QoC.

2008-01-01

448

PIC Simulations Of Ion Acceleration By Linearly And Circularly Polarized Laser Pulses

PET and MRI in cardiac imaging: from validation studies to integrated applications

Linearly polarized laser radiation accelerates electrons to very high velocities and these electron form a sheath layer on the rear side of thin targets where preferentially protons are accelerated. When mass-limited targets are used, the lateral transport of the absorbed laser energy is reduced and the accelerating field is enhanced. For targets consisting of two ion species, heavier ions facilitate formation of quasi-monoenergetic bunch of lighter ions. For circularly polarized light, fast electron production is suppressed by the absence of the oscillatory component of the ponderomotive force. Ions are accelerated on the front side by the separation field and very thin foil can be accelerated as one massive quasi-neutral block. As all ion species acquire the same velocity, this acceleration mechanism is preferred for heavier ions.

2008-06-24

449

PDBpaint, a visualization webservice to tag protein structures with sequence annotations

Positron emission tomography (PET) is the gold standard for non-invasive assessment of myocardial viability and allows accurate detection of coronary artery disease by assessment of myocardial perfusion. Magnetic resonance imaging (MRI) provides high resolution anatomical images that allow accurate evaluation of ventricular structure and function together with detection of myocardial infarction. Potential hybrid PET/MR tomography may potentially facilitate the combination of information from these imaging modalities in cardiology. Furthermore, the combination of anatomical MRI images with the high sensitivity of PET for detecting molecular targets may extent the application of these modalities to the characterization of atherosclerotic plaques and to the evaluation of angiogenetic or stem cell therapies, for example. This article reviews studies using MRI and PET in parallel to compare their performance in cardiac applications together with the potential benefits ...

2009-03-01

450

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

2011-01-01

451

Negotiating equity for management of DOE wastes

Needs, opportunities, and options for large scale systems research

One important factor frustrating optimal management of Department of Energy (DOE)-complex wastes is the inability to use licensed and permitted facilities systematically. Achieving the goal of optimal use of DOE`s waste management facilities is politically problematic for two reasons. First, no locale wants to bear a disproportionate burden from DOE wastes. Second, the burden imposed by additional wastes transported from one site to another is difficult to characterize. To develop a viable framework for equitably distributing these burdens while achieving efficient use of all DOE waste management facilities, several implementation and equity issues must be addressed and resolved. This paper discusses stakeholder and equity issues and proposes a framework for joint research and action that could facilitate equity negotiations among stakeholder and move toward a more optimal use of DOE`s waste management capabilities.

1994-09-01

452

Multimodal MRI assessment of damage and plasticity caused by status epilepticus in the rat brain

The Office of Energy Research was recently asked to perform a study of Large Scale Systems in order to facilitate the development of a true large systems theory. It was decided to ask experts in the fields of electrical engineering, chemical engineering and manufacturing/operations research for their ideas concerning large scale systems research. The author was asked to distribute a questionnaire among these experts to find out their opinions concerning recent accomplishments and future research directions in large scale systems research. He was also requested to convene a conference which included three experts in each area as panel members to discuss the general area of large scale systems research. The conference was held on March 26--27, 1984 in Pittsburgh with nine panel members, and 15 other attendees. The present report is a summary of the ideas presented and the recommendations proposed by the attendees.

1984-10-01

453

British Library Electronic Table of Contents (United Kingdom)

Summary Status epilepticus or other brain-damaging insults launch a cascade of events that may lead to the development of epilepsy. MRI techniques available today, including T2- and T1-weighted imaging, functional MRI, manganese enhanced MRI (MEMRI), arterial spin labeling (ASL), diffusion tensor imaging (DTI), and phase imaging, can detect not only damage caused by status epilepticus but also plastic changes in the brain that occur in response to damage. Optimal balance between damage and recovery processes is a key for planning possible treatments, and noninvasive imaging has the potential to greatly facilitate this process and to make personalized treatment plans possible.

2011-01-01

454

Magnetic layer formation on plasma nitrided CoCrMo alloy

British Library Electronic Table of Contents (United Kingdom)

In this study structural and magnetic character of the expanded austenite phase (gN) layer formed on a medical grade CoCrMo alloy by a low-pressure Radio-Frequency plasma nitriding process was investigated. The formation of the expanded austenite phase is facilitated at a substrate temperature near 400^oC for 1, 2, 4, 6 and 20h under a gas mixture of 60% N2-40% H2. The magnetic state of the gN layers was determined by a surface sensitive technique, magneto-optic Kerr effect (MOKE), and with a scanning probe microscope in magnetic force mode (MFM). Strong evidence for the ferromagnetic nature of the gN-(Co,Cr,Mo) phase is provided by the observation of stripe domain structures and the hysteresis loops. The ferromagnetic state for the gN phase observed here is mainly linked to large lattice ...

2011-01-01

455

Low-level Waste Forum meeting report. Winter meeting, January 26--28, 1994

Landslides on ancient embankments in the Kinki district (Japan): Strong motion seismoscope of the 1596 Keichou-Fushimi earthquake

The Low-Level Radioactive Waste Forum is an association of representatives of states and compacts established to facilitate state and compact commission implementation of the Low-Level Radioactive Waste Policy Act of 1980 and the Low-Level Radioactive Waste Policy Amendments Act of 1985 and to promote the objectives of low-level radioactive waste regional compacts. The Forum provides an opportunity for states and compacts to share information with one another and to exchange views with officials of federal agencies. The Forum participants include representatives from regional compacts, designated host states, unaffiliated states, and states with currently-operating low-level radioactive waste facilities. This report contains information synthesizing the accomplishments of the Forum, as well as any new advances that have been made in the management of low-level radioactive wastes.

1994-12-31

456

British Library Electronic Table of Contents (United Kingdom)

Landslides on ancient embankments, burial mounds of Imashiro-zuka and Nishimotome-zuka, induced by historical earthquakes related to the tectonic movement of active fault systems located between northern Osaka and Kobe, are discussed. The geological conditions of the foundation of the mounds, the lithology, stiffness, and the position of the mound relative to the fault systems, contributed to the collapse of the mounds. Obvious interior structures of landslides revealed the inherent deformation process, and provided significant insight into landslide mechanisms. Rapid increasing pore water pressure was necessary to facilitate sliding along the almost horizontal slip surfaces developed in the main part of the landslide bodies on the Imashiro-zuka mound. Liquefaction analysis using both the ...

2011-01-01

457

JMassBalance: mass-balanced randomization and analysis of metabolic networks

British Library Electronic Table of Contents (United Kingdom)

Summary: Analysis of biological networks requires assessing the statistical significance of network-based predictions by using a realistic null model. However, the existing network null model, switch randomization, is unsuitable for metabolic networks, as it does not include physical constraints and generates unrealistic reactions. We present JMassBalance, a tool for mass-balanced randomization and analysis of metabolic networks. The tool allows efficient generation of large sets of randomized networks under the physical constraint of mass balance. In addition, various structural properties of the original and randomized networks can be calculated, facilitating the identification of the salient properties of metabolic networks with a biologically meaningful null model. Availability and Imp...

2011-01-01

458

Intravaginal immunization using the recombinant HIV-1 clade-C trimeric envelope glycoprotein CN54gp140 formulated within lyophilized solid dosage forms

British Library Electronic Table of Contents (United Kingdom)

Vaccine-mediated prevention of primary HIV-1 infection at the heterosexual mucosal portal of entry may be facilitated by highly optimised formulations or drug delivery devices for intravaginal (i.vag) immunization. Previously we described hydroxyethylcellulose (HEC)-based rheologically structured gel vehicles (RSVs) for vaginal immunization of an HIV-1 vaccine candidate, a soluble recombinant trimeric HIV-1 clade-C envelope glycoprotein designated CN54gp140. Here we investigated the efficacy of lyophilized solid dosage formulations (LSDFs) for prolonging antigen stability and as i.vag delivery modalities. LSDFs were designed and developed that upon i.vag administration they would reconstitute with the imbibing of vaginal fluid to mucoadhesive, site-retentive semi-solids. Mice were immunize...

2011-01-01

459

Information security in networked supply chains: impact of network vulnerability and supply chain integration on incentives to invest

British Library Electronic Table of Contents (United Kingdom)

Recent supply chain reengineering efforts have focused on integrating firms? production, inventory and replenishment activities with the help of communication networks. While communication networks and supply chain integration facilitate optimization of traditional supply chain functions, they also exacerbate the information security risk: communication networks propagate security breaches from one firm to another, and supply chain integration causes breach on one firm to affect other firms in the supply chain. We study the impact of network security vulnerability and supply chain integration on firms? incentives to invest in information security. We find that even though an increase in either the degree of network vulnerability or the degree of supply chain integration increases the secur...

2010-01-01

460

Influence of sulfur dioxide content of 20% and 92% sulfuric acid on anodic behavior of stainless steels

Incorporation of organizational failures in PSA

This paper investigates steels 12Kh18N10T, 10Kh17N13M2T, 08Kh22N6T, and 08Kh21N6M2T. In corrosion of stainless steels in sulfuric acid solutions, dissolved sulfur dioxide acts as a cathodic depolarizer, capable of being reduced to elemental sulfur or forming sulfides with the metal. Depending on the conditions, this is associated with some increase in potential and greater or lesser increase in corrosion or facilitation of passivation of active steel. After passivation the influence of SO/sub 2/ is negligible. The results of this investigation were used as initial data for developing systems of anodic protection for shell-and-tube heat exchangers for sulfuric acid.

1986-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R): Localization to chromosome band 7p14 and integration into the cytogenetic, physical, and genetic map of chromosome 7

A large portion of the work performed at nuclear power plants follows standardized flow paths. For example, although components on which the maintenance crew works differ from one assignment to the next, all assignments basically follow the same process: requesting, reviewing, planning, scheduling, executing, testing, and documenting the work. In general, the term open-quotes work processclose quotes is used to refer to a standardized sequence of tasks designed within the operational environment of an organization to achieve a specific goal. The predictable nature of work processes suggests that a systematic analysis can be conducted to identify the desirable design of the process and to develop performance measures with respect to the strengths and weaknesses in the process. Furthermore, because of the close relationship of the work process to plant performance and plant safety, it is believed that such an analysis will facilitate the inclusion of organizational ...

1993-06-20

462

British Library Electronic Table of Contents (United Kingdom)

The gene encoding the human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R1) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human/rodent somatic cell hybrid mapping panel. This assignment was confirmed and the gene localized to chromosome band 7p14 by fluorescence in situ hybridization. A yeast artificial chromosome containing ADCYAP1R1 was identified in the CEPH {open_quotes}B{close_quotes} Mega-YAC library. This YAC includes two highly polymorphic dinucleotide repeat sequences that will facilitate genetic studies of the contribution of ADCYAP1R1 in disease states of the central nervous and neuroendocrine systems. 13 refs., 1 fig.

1994-10-01

463

How an IPO Helps in M&A

An initial public offering (IPO) can often provide a powerful stimulus to private companies seeking to pursue an acquisition-driven growth strategy. Based on a comprehensive analysis of U.S. IPOs, the authors show that newly public companies are prolific acquirers. Over 30% of companies conducting an IPO make at least one acquisition in their IPO year, and the typical IPO firm makes about four acquisitions during its first five years as a public company. IPOs facilitate M&A not only by providing infusions of capital but also by creating ongoing access to equity and debt markets for cash-financed deals. In addition, IPOs create an acquisition currency that can prove valuable in stock-financed deals when the shares are attractively priced. The authors also argue that IPOs improve the ability...

2010-01-01

464

Harnessing competence and confidence: Dimensions in education and development for advanced and consultant practice

Global challenges in energy

Consultant and advanced practice are relatively new concepts in radiography. There is evidence to show that as the career progression framework is being adopted the numbers of consultant and advanced practitioners in radiography are growing with the latter growing at a faster rate. The article considers the concept of advanced and consultant practice and the education requirements to support development. Preparation for an advanced practice role begins at the practitioner stage. Masters' level programmes are available to support the development of advanced practice. Education needs to be flexible as new advanced practice roles emerge. It is necessary to take practitioners beyond a defined modality to include leadership and people skills. These are essential for those aspiring to become consultants. Consultants require a high level of clinical knowledge for expert practice but also strategic vision and interpersonal intelligence to facilitate leadership and ...

2008-12-15

465

Generation and Characterization of Monoclonal Antibodies to Human Keratinocyte Growth Factor Receptor

Environmental and security concerns are stimulating global interest in hydrogen power, renewable energy, and advanced transportation technologies, but no significant movement away from oil and a carbon-based world economy is expected soon. Over the longer-term, however, a transition from fossil fuels to a non-carbon-based economy will likely occur, affecting the type of environment future generations may encounter. Key challenges will face the world's energy industry over the next few decades to ensure a smooth transition-challenges which will require government and industry solutions beginning as early as today. This paper identifies four critical challenges in energy and the choices which will have to be made on how best to confront growing pollution caused by fossil fuels and how to facilitate an eventual revolutionary-like transition to a non-carbon-based global economy.

2006-10-01

466

British Library Electronic Table of Contents (United Kingdom)

Keratinocyte growth factor receptor (KGFR) and fibroblast growth factor receptor (FGFR) 2c share identical amino acid sequences, except for a 46-amino acid domain in the extracellular region. Monoclonal antibodies (MAbs) specific to KGFR have not been reported nor are commercially available. In this study, we generated murine MAbs specific to KGFR in non-obese diabetic (NOD) mice using a modified Repeated Immunizations at Multiple Sites (RIMMS) technology. Stable cell lines expressing the full-length human KGFR or FGFR2c were produced to facilitate the identification of KGFR-specific MAbs. Following the initial screening of hybridoma clones with a fluorescence-based, confocal cell detection method and ELISA, KGFR-specific MAbs were selected and confirmed by flow cytometry and Western blot ...

2006-01-01

467

Electrospun porous SnO2 nanotubes as high capacity anode materials for lithium ion batteries

British Library Electronic Table of Contents (United Kingdom)

Porous SnO2 nanotubes were prepared via electrospinning followed by calcination in air. As anode materials for lithium ion batteries, the porous nanotubes delivered a high discharge capacity of 807mAhg^-^1 after 50cycles. Even after cycled at high rates, the electrode still retained a high fraction of its theoretical capacity. Such excellent performances of porous SnO2 nanotubes could be attributed to the porous and hollow structure which facilitated liquid electrolyte diffusion into the bulk materials and buffered large volume changes during lithium ions insertion/extraction. Furthermore, the nanoparticles of nanotubes provided the shorter diffusion length for lithium ions insertion which benefited in retaining the structural stability and good rate performance. Our results demonstrated t...

2010-01-01

468

Efficient methodologies for sensitive HIV-1 RNA quantitation from plasma and vaginal secretions

British Library Electronic Table of Contents (United Kingdom)

Background: Quantifying HIV levels in mucosal secretions is essential to study compartmentalized expression of HIV and facilitate development of intervention strategies to prevent disease progression and transmission. Objectives: To develop a sensitive, reliable, and cost-effective technique to quantify HIV from blood and vaginal secretions that is compatible with efficient implementation in clinical research environments. Study design: A sensitive, reliable, internally-controlled real-time reverse transcriptase (RT) PCR assay, which uses the HIV-1 pol gene as a target (Hpol assay) was developed to quantify HIV levels in plasma and genital secretions, and compared to the widely used Roche Amplicor(TM) HIV-1 Monitor assay. In addition, a simplified method of sample collection and processing...

2009-01-01

469

Direct observation of polymerization in the oleic acid-ozone heterogeneous reaction system by photoelectron resonance capture ionization aerosol mass spectrometry

British Library Electronic Table of Contents (United Kingdom)

High molecular weight products of the ozonolysis reaction of particle-phase 9-octadecenoic acid (oleic acid) have been studied by photoelectron resonance capture ionization (PERCI) mass spectrometry (MS). Oleic acid particles ( Formula Not Shown , Formula Not Shown ) were reacted with ozone (1.8x10-4atm) in a flow reactor at reaction times of 8 and 23s. Particles were sampled on-line with a differentially pumped particle inlet and chemically analyzed by PERCI-MS. PERCI is a soft ionization method that permits the direct measurement of relatively high molecular weight compounds, facilitating molecular identification. In addition to cyclic oxygenates, such as secondary ozonides and geminal diperoxides that were reported previously, we demonstrate the formation of polymers at the particle sur...

2006-01-01

470

Diagnostic and Prognostic Utility of t(14;18) in Follicular Lymphoma

British Library Electronic Table of Contents (United Kingdom)

Abstract Background: Follicular lymphoma (FL) is one of the most common non-Hodgkin's lymphomas of B cells, being closely associated with a t(14;18) translocation. Detection of t(14;18), which is present in 70-95% of FL, might aid in FL diagnosis. Objective: To compare the efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples of FL patients at different stages. Combined with other immunophenotypic biological determinants, detection of t(14;18) might help to determine patients at increased risk according to the FL International Prognostic Index (FLIPI) and therefore facilitate appropriate treatment. Design and Methods: This study was mainly based on a retrospective examination of...

2007-01-01

471

Development of anion-exchange resins for separations of actinides

Development and operational experience of traveling in core probe drive for flux scan of 540 MWe PHWR of Tarapur

The evolution of these new ion-exchange resins hinges upon an understanding of the chemistry of actinide metal complexes as they exist in solution and when interacting with the cationic sites. Molecular modeling of electrostatic interactions between the metal complex and the cationic substrate is used to help visualize uptake mechanisms and, eventually, to predict optimized coordination sites. Thus, these new materials integrate the fields of ion-specific chelation and ion-exchange technology. Synthesized resins contain cationic sites designed to facilitate the uptake of plutonium nitrato complexes from high-nitrate solutions, and they exhibit distribution coefficients that are up to 10 times higher than those observed for commercial resins. Additionally, some new resins show surprisingly large Kd`s for anionic complexes of Am(III) and U(VI).

1997-12-31

472

Design of sediment-control measures for small areas in surface coal mining. Draft report

TAPP-3 and 4 reactors use large number of Self Powered Neutron Detectors (SPNDs) for Neutronic lower measurement and control. To perform in-situ calibration of these detectors in select locations and to validate the reactor physics codes which predict flux at various points in the core, traveling in-core probes (TIP) are required. The TIP assembly consists of a miniature neutron sensitive detector. The detector is driven in and out of core using a mechanism which facilitates positioning of the detector anywhere inside a vertical tube (Central carrier tube of any of the six select Vertical Flux Units) in the core. TIP is driven through retractable feed mechanism for a stroke of 13 m. This paper describes the developmental efforts and the operational feedback of the retractable feed mechanism for the stroke of 13 m used at TAPP 3 and 4 reactor. (author)

2006-11-13

473

Design of sediment-control measures for small areas in surface coal mining

The manual provides design information for four groups of sediment control structures and treatments which are suitable for use on small areas in surface coal mining. A detailed design procedure is given for each group which will estimate the total amount of settleable solids from a small area with a sediment control structure or treatment for a specific design storm. A procedure is also given for estimating the mean annual sediment yield from a small area. Information on design, installation, maintenance and cost is given for each sediment control measure. Methods for determining the maximum allowable area for each sediment control measure and for design of combined sediment control systems are presented. The design procedures contain numerous nomographs, graphs, and worksheets to facilitate the use of the design procedure. The sediment control measures, design procedures, and materials in the manual are applicable to coal-bearing regions of the continental United ...

1982-04-01

474

Design of a magnetic braking system

The manual provides design information for four groups of sediment control structures and treatments which are suitable for use on small areas in surface coal mining. A detailed design procedure is given for each group which will estimate the total amount of settleable solids from a small area with a sediment control structure or treatment for a specific design storm. A procedure is also given for estimating the mean annual sediment yield from a small area. Information on design, installation, maintenance and cost is given for each sediment control measure. Methods for determining the maximum allowable area for each sediment control measure and for design of combined sediment control systems are presented. The design procedures contain numerous nomographs, graphs, and worksheets to facilitate the use of the design procedure. The sediment control measures, design procedures, and materials in the manual are applicable to coal bearing regions of the continental Unted ...

1982-08-01

475

Deposition of amino-rich coatings by RF magnetron sputtering of Nylon: Investigation of their properties related to biomedical applications

A non-contact method, using magnetic drag force principle, was proposed to design the braking systems to improve the shortcomings of the conventional braking systems. The extensive literature detailing all aspects of the magnetic braking is briefly reviewed, however little of this refers specifically to upright magnetic braking system, which is useful for industries. One of the major issues to design upright magnetic system is to find out the magnetic flux. The changing magnetic flux induces eddy currents in the conductor. These currents dissipate energy in the conductor and generate drag force to slow down the motion. Therefore, a finite element model is developed to analyze the phenomena of magnetic flux density when air gap and materials of track are varied. The verification shows the predicted magnetic flux is within acceptable range with the measured value. The results will facilitate the design of magnetic braking systems.

2006-09-01

476

British Library Electronic Table of Contents (United Kingdom)

Amino-rich polymeric coatings are widely used in biomedical applications, since they promote adsorption of diverse biomolecules or facilitate cell growth. As a consequence, there is a growing interest in fabrication of such coatings that is focused predominantly on the optimization of the deposition process in terms of high density of primary amino groups. In addition, the nature of biomedical applications requires also sufficient stability of the films in aqueous environments. This aspect is investigated in this contribution. In particular, the effect of water and phosphate buffer saline on the coatings prepared by RF magnetron sputtering of Nylon 6,6 in Ar/N2 and N2/H2 gas mixtures is evaluated. The samples exposed to liquids are characterized by various diagnostic methods and their prop...

2011-01-01

477

Degradation Improves Tissue Formation in (Un)Loaded Chondrocyte-laden Hydrogels

British Library Electronic Table of Contents (United Kingdom)

Background Photopolymerizable poly(ethylene glycol) (PEG) hydrogels offer a platform to deliver cells in vivo and support three-dimensional cell culture but should be designed to degrade in sync with neotissue development and endure the physiologic environment. Questions/purposes We asked whether (1) incorporation of degradation into PEG hydrogels facilitates tissue development comprised of essential cartilage macromolecules; (2) with early loading before pericellular matrix formation, the duration of load affects matrix production; and (3) dynamic loading in general influences macroscopic tissue development. Methods Primary bovine chondrocytes were encapsulated in hydrogels (n?=?3 for each condition). The independent variables were hydrogel degradation (nondegrading PEG and degrading olig...

2011-01-01

478

Cost and sensitivity analysis for uranium in situ leach mining. Open file report Oct 79-Mar 81

Cost Estimation for Decommissioning. An International Overview of Cost Elements, Estimation Practices and Reporting Requirements

This report presents the results of an assessment of uranium in situ leach mining costs through the application of process engineering and discounted cash flow analysis procedures. A computerized costing technique was developed to facilitate rapid cost analyses. Applications of the cost model will generate mine life capital and operating costs as well as solve for economic production cost per pound U/sub 3/O/sub 8/. Conversely, rate of return may be determined subject to a known selling price. The data bases of the cost model were designed to reflect variations in Texas versus Wyoming site applications. The results of applying the model under numerous ore deposit, operating, well field, and extraction plant conditions for Texas and Wyoming are summarized in the report. Sensitivity analysis of changes in key project parameters have also been tested and are included.

1981-03-01

479

British Library Electronic Table of Contents (United Kingdom)

This report is based on a study carried out by the NEA Decommissioning Cost Estimation Group (DCEG) on decommissioning cost elements, estimation practices and reporting requirements. Its findings indicate that cost methodologies need to be updated continuously using cost data from actual decommissioning projects and hence, systematic approaches need to be implemented to collect these data. The study also concludes that changes in project scope may have the greatest impact on project costs. Such changes must therefore be identified immediately and incorporated into the estimate. Finally, the report notes that more needs to be done to facilitate the comparison of estimates, for example by providing a reporting template for national estimates. (authors)

480

A red basil cell line (T2b) rich in rosmarinic acid (RA) was selected for the stable production of anthocyanins (ACs) in the dark. Cell suspension cultures were subjected to mechanical stress through increased agitation (switch from 90 to 150rpm) to determine the relationship between AC and RA accumulation. Cell extracts were analyzed by HPLC and LC-MS, and the resulting data were processed with multivariate statistical analysis. MS and MS/MS spectra facilitated the putative annotation of several complex cyanidin-based ACs, which were esterified with coumaric acid and, in some cases, also with malonic acid. It was also possible to identify various RA-related molecules, some caffeic and coumaric acid derivatives and some flavanones. Mechanical stress increased the total AC and RA contents, ...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Coordinated control of multiple manipulator systems

Conformational analysis of ethyl azidoformate

A scheme for controlling multimanipulator systems is presented. The control objective is to coordinate the manipulators to perform parts-matching tasks such as screwing a nut onto a bolt. The task of moving a rigid object can be treated as a special case. Two secondary control objectives internal force control and load distribution can be accomplished within the structure of the control law. The internal force control mechanism keeps the internal forces on the object being manipulated at a desirable level. The load distribution mechanism distributes control effort to each manipulator according to a weighting factor. It is also shown that the control algorithm has a modular structure which facilitates its implementation on a multiprocessor computer. The scheme was tested on a planar scara type dual-manipulator system. A series of experimental results is included to demonstrate the system performance under various conditions.

1993-08-01

482

Computational and experimental studies on the forming of KSTAR superconducting magnet coil

A conformational analysis of ethyl azidoformate (EAF) has been carried out by the MINDO/3 quantum-chemical method. It has been shown that EAF exists in the form of two conformers differing with respect to rotation around the C-N bond. Complete optimization of the geometry has been carried out for both conformers. It has been found that the transoid conformation is planar and that the cisoid conformation is nonplanar. The height of the rotation barrier is 15.4 kcal/mole. The optimal geometry of the transition state has been calculated. It has been noted that a significant role in the mechanism of the conformational transition is played by the inversion of a nitrogen, which facilitates the transition. The results of the calculation have been confirmed by IR-spectroscopic data.

483

Computational and experimental studies on the forming of KSTAR superconducting magnet coil

The plastic deformation behavior of formed CICC for the superconducting Tokamac fusion device was examined and appropriate manufacturing information was provided. A relation between travel of the bending roller and spring back displacement was obtained via virtual manufacturing. The radius of CICC after forming was expressed as a function of the bend-roll travel. The maximum von Mises stress after spring back was also monitored for the SAGBO prediction. Next, the variation of the CICC cross-sectional area was examined during the first turn and during conduit bending with the largest curvature. Finally, the coil radius was measured and compared with the data generated from the virtual manufacturing. The measured data showed similar pattern as predicted one. Using the mapping function found to match with the real data, the data from the virtual manufacturing may facilitate accurate manufacturing.

2001-07-01

484

Characteristics of batch rotor-stator mixer performance elucidated by shaft torque and angle resolved PIV measurements

The plastic deformation behavior of formed CICC for the superconducting Tokamac fusion device was examined and appropriate manufacturing information was provided. A relation between travel of the bending roller and spring back displacement was obtained via virtual manufacturing. The radius of CICC after forming was expressed as a function of the bend-roll travel. The maximum von Mises stress after spring back was also monitored for the SAGBO prediction. Next, the variation of the CICC cross-sectional area was examined during the first turn and during conduit bending with the largest curvature. Finally, the coil radius was measured and compared with the data generated from the virtual manufacturing. The measured data showed similar pattern as predicted one. Using the mapping function found to match with the real data, the data from the virtual manufacturing may facilitate accurate manufacturing.

2001-06-27

485

British Library Electronic Table of Contents (United Kingdom)

Abstract Characteristics of batch rotor-stator mixer performance are elucidated by shaft torque and angle resolved 2D PIV measurements obtained in a full-scale, custom build, bottom-mounted, rotor-stator mixer unit operating in the turbulent regime with water as working fluid. Measurements have been acquired at various rotor speeds corresponding to impeller based Reynolds numbers between 2.0--105 and 8.5--105. The use of a transparent Plexiglas stator facilitated PIV measurements inside and outside the stator as well as into the stator slots themselves. The governing mechanisms controlling the complex flow structures, flow rates, power dissipation, velocity fields, strain rate fields and turbulence intensity fields are explained, highlighting the influence on rotor-stator mixer performance...

2011-01-01

486

Business-Government Relations and Industrial Change: The Politics of Upgrading in Morocco and Tunisia

British Library Electronic Table of Contents (United Kingdom)

SummaryProcesses of upgrading and the promotion of industrial "clusters," which encompass multiple policies, institutions, and actors, necessarily vary from place to place. Based on the evidence from Morocco and Tunisia, this article contends that different patterns of business-government relations facilitate different elements of industrial upgrading and cluster promotion. State-dominated political economies may be more amenable to implementing macroeconomic and infrastructure-related measures, which are critical in the initial stages of upgrading. Political economies with more organized business communities may be better equipped to pursue the kinds of inter-firm linkages traditionally associated with later phases of upgrading, when the focus is on boosting innovation.

2007-01-01

487

Biomass production and relative competitiveness of a C3 legume and a C4 grass co-dominant in the semiarid Loess Plateau of China

British Library Electronic Table of Contents (United Kingdom)

Bothriochloa ischaemum L. and Lespedeza davurica (Laxm.) Schindl. are two co-dominant species of great importance in reducing soil and water loss and maintaining the distinctive natural scenery of the semiarid Loess Plateau of China. Our aim was to determine the growth and interspecific competition between these species under water stress to facilitate the prediction of community succession and guide the selection of appropriate methods of conservation and use in the area. A pot experiment was designed to investigate the effects of water stress and competition on biomass production and allocation, relative competitive ability and water use efficiency of the two species. Bothriochloa ischaemum (a C4 perennial herbaceous grass) was planted in the same pot with L. davurica (a C3 perennial leg...

2011-01-01

488

Biological scarcity: looking beyond regulatory exteriors in Taiwan

British Library Electronic Table of Contents (United Kingdom)

Research and bioethical policies are increasingly seen to facilitate, rather than to impede, scientific progress. Bioethical structures, nonetheless, often fail to account for or to shape the specific social surrounds in which scientific research is conducted. In Taiwan, a perceived shortage in human biological donations threatens to hamper stem cell research progress. This paper considers the stalled national biobank project and the lack of a reliable embryo supply for stem cell research, and suggests that one must look beyond regulatory structures in seeking explanations for such shortages. Specific aspects of Taiwan's democratization and social changes combine with problematic relations between patients and physicians to shape the terrain upon which human biologicals are given or withhe...

2011-01-01

489

Arterial stiffness is associated with low thigh muscle mass in middle-aged to elderly men

British Library Electronic Table of Contents (United Kingdom)

Objective: Sarcopenia of legs is an important cause of physical dysfunctions, frailty and dependence. Many predisposing and underlying mechanisms of sarcopenia, including age, sedentary life style, oxidative stress, insulin resistance, and low testosterone levels, are also known to be related to atherosclerosis, which is another leading cause of morbidity and mortality in elderly subjects. In this study, we investigated our hypothesis that sarcopenia and atherosclerosis are associated with each other to facilitate mutual abnormalities. Methods: Study was performed in apparently healthy 496 middle-aged to elderly persons recruited consecutively among the visitors to the medical check-up program, Anti-Aging Doc, in a University hospital, from March 2006 to December 2007. Mid-thigh muscle cro...

2010-01-01

490

Application of microvascular free osteocutaneous flaps in the management of post-radiation recurrent oral cancer

Adventure based counselling, individual counselling and object relations: a critical evaluation of a qualitative study

Fifty-nine patients underwent free flap osteocutaneous reconstruction that consisted of flaps of the dorsum of the foot in 26 patients and iliac crest flaps in 33 with a success rate of 92 percent and a mortality rate of 1.6 percent. These flaps, which require the expertise of microvascular surgeons, are time-consuming and complicate operating room and time management, but they represent a remarkable advance in reconstruction that can facilitate cosmetic and functional recovery of the patient. In particular, they promote healing in radiation-recurrent oral cancer and represent a definitive form of management for established radionecrosis of the mandible. The large volume of tissue available with iliac crest osteocutaneous grafts permits the management of patients with extensive cancer involving the skin, mucosa, and bone, but cancer control may still be disappointing and there is a need for improved adjuvant chemotherapy protocols. This technique appears to be a ...

1985-10-01

491

British Library Electronic Table of Contents (United Kingdom)

Adventure based counselling is a short-term experiential psychotherapeutic approach, which utilises adventurous activities and being in natural environmental in order to facilitate therapeutic change in clients. The present paper critically appraises the results of a qualitative study that investigated how clients with self-reported anxiety and depression experienced participating in an innovative counselling intervention with combined individual counselling with such an adventurous outdoor transaction. Interpretative Phenomenological Analysis, as described by Smith and Osborn (2008), was used in order record and analyse the experiences of four male and six female students who were treated at the Teesside's University Counselling Service. Interviewees perceived the counselling sessions as ...

2010-01-01

492

Adaptive power-system stabilizer based on turbine governor control

A novel adaptive stabilizer, called an Adaptive Governor Power System Stabilizer (AGPSS), which operated on the governor turbine system, was described. There has been extensive research on adaptive excitation control of generators in power systems with the goal of achieving better dynamic performance. AGPSS tracks the frequency and damping of oscillations in a power system, and adjusts to ensure that there is always sufficient damping in the system. There is no interaction between stabilizers on different generating units, and each one acts independently, consuming oscillation energy locally. These stabilizers are easy to design and this facilitates their use in multimachine systems. Results obtained by nonlinear simulation of a single-machine and a three-machine power system were discussed, illustrating the effectiveness of the stabilizers in supplying damping and improving stability. It was pointed out that the operation of AGPSS does not conflict with that of ...

1994-12-31

493

Achieving success abroad

Abscisic Acid in Soil Facilitates Community Succession in Three Forests in China

Department of Foreign Affairs and International Trade services designed to assist businesses to effectively operate in global markets, were described. The goal of the Department is to give strategic targeted information that extends beyond export opportunities to include market access, investment, strategic alliances, technology transfer and competitiveness issues. These services constitute the Department`s Trade Commissioner Service, a network of 600 officers, deployed in 128 embassies and consulates worldwide to support Canadian business development initiatives across 16 sectors of the government`s International Trade Business Plan (ITBP). The Trade Commissioner Service programs include the WIN exporters database, a trade inquiries sourcing team, International Financial Institutions (IFI) projects, and the Program for Export Market Development (PEMD). A series of Sectoral Advisory Groups on International Trade (SAGIT) have been established to facilitate the needs ...

1995-12-31

494

British Library Electronic Table of Contents (United Kingdom)

Plants release secondary metabolites into the soil that change the chemical environment around them. Exogenous abscisic acid (ABA) is an important allelochemical whose role in successional trajectories has not been examined. We hypothesized that ABA can accumulate in the soil through successional processes and have an influence on forest dynamics. To this end, we investigated the distribution of ABA in forest communities from early to late successional stages and the response of dominant species to the gradient of ABA concentrations in three types of forests from northern to southern China. Concentrations of ABA in the soils of three forest types increased from early to late successional stages. Pioneer species? litters had the lowest ABA content, and their seed germination and seedling ea...

2011-01-01

495

A signature of six genes highlights defects on cell growth and specific metabolic pathways in murine and human hepatocellular carcinoma

British Library Electronic Table of Contents (United Kingdom)

Hepatocellular carcinoma (HCC) represents a major health problem as it afflicts an increasing number of patients worldwide. Albeit most of the risk factors for HCC are known, this is a deadly syndrome with a life expectancy at the time of diagnosis of less than 1?year. Definition of the molecular principles governing the neoplastic transformation of the liver is an urgent need to facilitate the clinical management of patients, based on innovative methods to detect the disease in its early stages and on more efficient therapies. In the present study, we have combined the analysis of a murine model and human samples of HCC to identify genes differentially expressed early in the process of hepatocarcinogenesis, using a microarray-based approach. Expression of 190 genes was impaired in murine ...

2011-01-01

496

A partnership approach to service needs assessment with family caregivers of an aging relative living at home: A qualitative analysis of the experiences of caregivers and practitioners

British Library Electronic Table of Contents (United Kingdom)

Background: As the global population ages, support for family caregivers who provide the bulk of care to community-dwelling older people is becoming ever more important. However, in many countries, homecare-service practitioners currently do not follow a systematic approach to assessing and responding to caregiver needs. Objectives: The aim of this study was to explore the experiences of caregivers and practitioners who took part in a field test of the Family Caregivers Support Agreement (FCSA) tool, a modified version of the Carers Outcome Agreement Tool (COAT) initially developed as the result of an Anglo-Swedish study. Both the COAT and the FCSA are designed to facilitate partnerships between caregivers and practitioners so that needs assessment and subsequent support services are negot...

2010-01-01

497

A non-uniformly sampled 4D HCC(CO)NH-TOCSY experiment processed using maximum entropy for rapid protein sidechain assignment

A Multi-Layered Image Cache for Scientific Visualization

One of the stiffest challenges in structural studies of proteins using NMR is the assignment of sidechain resonances. Typically, a panel of lengthy 3D experiments are acquired in order to establish connectivities and resolve ambiguities due to overlap. We demonstrate that these experiments can be replaced by a single 4D experiment that is time-efficient, yields excellent resolution, and captures unique carbon-proton connectivity information. The approach is made practical by the use of non-uniform sampling in the three indirect time dimensions and maximum entropy reconstruction of the corresponding 3D frequency spectrum. This 4D method will facilitate automated resonance assignment procedures and it should be particularly beneficial for increasing throughput in NMR-based structural genomics initiatives.

2010-05-01

498