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Sample records for ecological studies hsf

  1. Lessons from the use of genetically modified Drosophila melanogaster in ecological studies: Hsf mutant lines show highly trait-specific performance in field and laboratory thermal assays

    Sørensen, Jesper Givskov; Loeschcke, Volker; Kristensen, Torsten Nygård

    2009-01-01

    . 2.  We have tested the importance of inducible heat shock proteins (Hsps) under different thermal conditions using two heat shock factor (Hsf) mutant lines (either able (Hsf+) or unable (Hsf0) to mount a heat stress response) and an outbred laboratory adapted wild-type line of Drosophila...... melanogaster under both laboratory and field conditions.3.  In the field, there was a tendency towards better performance of Hsf+ flies relative to Hsf0 flies, but as compared with wild-type the performance of both mutant lines was very low.4.  In the laboratory tests, Hsf+ flies had higher heat knock......-down resistance relative to Hsf0 flies but in other assays on heat, cold and desiccation resistance there was either no difference between the two mutant lines or the Hsf0 line had higher performance. Also, the superiority of the wild-type flies under field conditions was trait specific.5.  The results emphasize...

  2. HSF1 overexpression enhances oncolytic effect of replicative adenovirus

    Deng Youwen

    2010-05-01

    Full Text Available Abstract Background E1B55kD deleted oncolytic adenovirus was designed to achieve cancer-specific cytotoxicity, but showed limitations in clinical study. To find a method to increase its efficacy, we investigated the correlation between oncolytic effect of such oncolytic adenovirus Adel55 and intracellular heat shock transcription factor 1 (HSF1 activity. Methods In the present study, human breast cancer cell line Bcap37 was stably transfected with constitutively active HSF1 (cHSF1 or HSF1 specific siRNA (HSF1i to establish increased or decreased HSF1 expression levels. Cytotoxicity of Adel55 was analyzed in these cell lines in vitro and in vivo. Furthermore, Adel55 incorporated with cHSF1 (Adel55-cHSF1 was used to treat various tumor xenografts. Results Adel55 could achieve more efficient oncolysis in cHSF1 transfected Bcap37 cells, both in vitro and in vivo. However, inhibition of HSF1 expression by HSF1i could rescue Bcap37 cell line from oncolysis by Adel55. A time course study of viral replication established a correlation between higher replication of Adel55 and cytolysis or tumor growth inhibition. Then, we constructed Adel55-cHSF1 for tumor gene therapy and demonstrated that it is more potent than Adel55 itself in oncolysis and replication in both Bcap37 and SW620 xenografts. Conclusions cHSF1 enhances the Adel55 cell-killing potential through increasing the viral replication and is a potential therapeutic implication to augment the potential of E1B55kD deleted oncolytic adenovirus by increasing its burst.

  3. 热休克转录因子1在肝细胞癌中的表达及意义%The study of expression of HSF1 primary hepatic carcinoma

    李袆; 王葵

    2009-01-01

    higher than that in its adjacent liver tissues.Both were involved in the primary hepatic carcinoma tumorigenesis.The mRNA expression of HSF1 were not associated with the patient′s gender,age,tumor size,pathologic grade or pathologic type,as well as level of serum AFP.It may create a new diagnostic method and treatment of PHC to study HSF1 further in primary hepatic carcinoma tumorigenesis.

  4. Ecological baseline studies

    Vickers, D.

    1980-01-01

    Environmental studies of Merritt Island are discussed. Five areas of the island's ecology are examined. They include: a terrestrial community analyses, a plant community study, a small mammal population study, a rainfall study, and an ichthyological analyses.

  5. Riluzole increases the amount of latent HSF1 for an amplified heat shock response and cytoprotection.

    Jingxian Yang

    Full Text Available BACKGROUND: Induction of the heat shock response (HSR and increased expression of the heat shock proteins (HSPs provide mechanisms to ensure proper protein folding, trafficking, and disposition. The importance of HSPs is underscored by the understanding that protein mis-folding and aggregation contribute centrally to the pathogenesis of neurodegenerative diseases. METHODOLOGY/PRINCIPAL FINDINGS: We used a cell-based hsp70-luciferease reporter gene assay system to identify agents that modulate the HSR and show here that clinically relevant concentrations of the FDA-approved ALS drug riluzole significantly increased the heat shock induction of hsp70-luciferse reporter gene. Immuno-Western and -cytochemical analysis of HSF1 show that riluzole increased the amount of cytosolic HSF1 to afford a greater activation of HSF1 upon heat shock. The increased HSF1 contributed centrally to the cytoprotective activity of riluzole as hsf1 gene knockout negated the synergistic activity of riluzole and conditioning heat shock to confer cell survival under oxidative stress. Evidence of a post-transcriptional mechanism for the increase in HSF1 include: quantitation of mRNA(hsf1 by RT-PCR showed no effect of either heat shock or riluzole treatment; riluzole also increased the expression of HSF1 from a CMV-promoter; analysis of the turnover of HSF1 by pulse chase and immunoprecipitation show that riluzole slowed the decay of [(35S]labeled-HSF1. The effect of riluzole on HSF1 was qualitatively different from that of MG132 and chloroquine, inhibitors of the proteasome and lysosome, respectively, and appeared to involve the chaperone-mediated autophagy pathway as RNAi-mediated knockdown of CMA negated its effect. CONCLUSION/SIGNIFICANCE: We show that riluzole increased the amount of HSF1 to amplify the HSR for cytoprotection. Our study provides novel insight into the mechanism that regulates HSF1 turnover, and identifies the degradation of HSF1 as a target for

  6. 热应激预处理影响鼠背轴型皮瓣HSP70、HSF1表达的实验研究%Study of Heat Stress Pretreatment Effect on Expressions of HSP70,HSF1 in Axial Flap of Rats

    刘哲伟; 苏开新; 陈军; 姚建; 李辉; 王海华; 赵鑫

    2014-01-01

    目的探讨热应激预处理(HSP)对缺血皮瓣组织的保护作用及其与皮瓣中热休克蛋白70(HSP70)和热休克因子1(HSF1)的关系。方法将96只雄性Sprague Dawley 大鼠随机分为缺血组与实验组(HSP+缺血组)。缺血组:皮瓣模型制备后,阻断皮瓣蒂部第1h、2h、4h、6h、8h、10h共6个时间点,每时间点后观测皮瓣存活面积、HSP70、HSF1的变化,并进行统计学分析;实验组( HSP+缺血组):先予以加热预处理,之后与缺血组相同。结果单纯缺血组皮瓣成活率下降率较实验组(HSP+缺血组)明显,差异有显著性(P<0.05),热应激预处理各组皮瓣的HSP70、HSF1合成量与皮瓣存活率呈正相关(r=0.7577,P<0.01)。结论热应激预处理能够改善皮瓣组织的存活率,HSP70、HSF1在热应激预处理改善皮瓣存活率的保护作用中发挥着重要的作用。%Objective This study was to reveal the continuous changes of HSP70 and HSF1 in the delayed and pro-tection of heat stress pretreatment on ischemic preconditioning axial flap in rats. Methods Animals were divided into two groups randomly:HSP+IPC group,ischemic pretreatment group( IPC) . In IPC group,rats skin flaps received ischemic pretreatment for 1 h,2 h,4 h,6 h,8 h,10 h,and the survival rate of the skin flaps,the contents of HSP70 and HSF1 were examined at the 6 time points. HSP+IPC group was given heat stress pretreatment and then the same treatment as IPC group. Results The survival rates of IPC descends more significantly than that of HSR+IPC. The survival rates of skin flaps have positive correlation with the contents of HSP70 and HSF1(r=0. 7577,P<0. 01). Conclusions Heat stress pretreatment can improve the survival rate of ischemia flap and the mechanism of the protection can be related to the func-tion of HSP70 and HSF1.

  7. Prokaryotic Expression and Purification of Heat Shock Factor HSF1 in Arabidopsis thaliana%拟南芥热激因子HSF1的表达与纯化

    郭丽红; 王定康; 袁燕; 刘开庆; 陈雪; 陈善娜

    2009-01-01

    [Objective] This study was to express and purify Arabidopsis thaliana heat shock factor HSF1. [Method] Using Escherichia coli M15 harboring HSF1 (pQE32/His6-HSF1, pREP4) as experimental materials, HSF1 was induced to express with isopropy1-β-D-galactoside (IPTG); then the expression product was purified using Ni-NTA-agarose affinity chromatography and analyzed by SDS-PAGE. [Result] HSF1 of Arabidopsis thaliana was successfully expressed and purified. [Conclusion] This study provides materials for understanding the blinding site of HSF1 on Arabidopsis thaliana chromosome, further laying a good foundation for revealing the regulatory mechanism and physiological function of HSF1.

  8. Audubon Ecology Study Program.

    National Audubon Society, New York, NY.

    The materials in the set include a student reader "The Story of Ecology," a leaders' guide, and a large, pictorial wall chart. The student reader is divided into 10 units relating to a definition of ecology, the sun and life, air and the water cycle, major divisions of the earth, plants and food chains, distribution of plants and animals,…

  9. 小鼠MEF/Hsf1-/-/Hsf1细胞系的重建及Hsf1,SV40T-ag蛋白的表达%Reconstitute MEF/Hsf1-/-/Hsf1 mouse cell line and expression of Hsf1, SV40-Tag proteins

    蒋杞英; 张智; 胡延忠; 王明丽; 马远方

    2014-01-01

    目的:重建热休克转录因子1(Heat shock transcription factor 1,Hsf1)过表达的MEF/Hsf1-/-/Hsf1细胞系,为进一步研究Hsf1的功能提供实验模型.方法:将携带Hsf1全长基因的逆转录病毒载体pWZL-blast-flag-Hsf1,通过瞬时转染的方法,转染产生病毒的小鼠包装细胞293 Phoenix.用病毒上清直接感染MEF/Hsf1-/-细胞,建立Hsf1稳定表达的MEF/Hsf1-/-/Hsf1细胞系.通过Western blot实验,检测Hsf1和SV40T抗原(T-antigen,T-ag)蛋白的表达.结果:Hsf1蛋白在MEF/Hsf1-/-/Hsf1细胞中的表达比WT/MEF细胞强,而在MEF/Hsf1-/-细胞中没有明显表达.SV40T-ag蛋白在MEF/Hsf1-/-细胞中的表达明显比WT/MEF细胞和MEF/Hsf1-/-/Hsf1细胞强,而SV40T-ag蛋白在WT/MEF细胞中的表达强于MEF/Hsf1-/-/Hsf1细胞.结论:成功建立了MEF/Hsf1-/-/Hsf1细胞系;Hsf1参与了对SV40T-ag蛋白的表达调控.

  10. Analysis of the Effects of HSF1 Inhibition in Colon Cancer

    Williams, Linda Marie

    2014-01-01

    The deleterious effects o f physiological and environmental stresses which provoke cell damage is defended by a fundamental cellular mechanism, the heat shock response (HSR), mediated by heat shock factor 1 (HSF1). Evidence suggests th a t genetic depletion of HSF1 dramatically protects mice from tumor formation mediated by RAS over-activation or p53 inactivation both commonly mutated in colorectal cancer. Here we studied the effects of silencing HSF1 in HCT116 wt, HCT p53 - /- and HKE3 colon...

  11. BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon heat stress

    Bcl2-associated athoanogene (BAG) 3 is a member of the co-chaperone BAG family. It is induced by stressful stimuli such as heat shock and heavy metals, and it regulates cellular adaptive responses against stressful conditions. In this study, we identified a novel role for BAG3 in regulating the nuclear shuttling of HSF1 during heat stress. The expression level of BAG3 was induced by heat stress in HeLa cells. Interestingly, BAG3 rapidly translocalized to the nucleus upon heat stress. Immunoprecipitation assay showed that BAG3 interacts with HSF1 under normal and stressed conditions and co-translocalizes to the nucleus upon heat stress. We also demonstrated that BAG3 interacts with HSF1 via its BAG domain. Over-expression of BAG3 down-regulates the level of nuclear HSF1 by exporting it to the cytoplasm during the recovery period. Depletion of BAG3 using siRNA results in reduced nuclear HSF1 and decreased Hsp70 promoter activity. BAG3 in MEF(hsf1−/−) cells actively translocalizes to the nucleus upon heat stress suggesting that BAG3 plays a key role in the processing of the nucleocytoplasmic shuttling of HSF1 upon heat stress. - Highlights: • The expression level of BAG3 is induced by heat stress. • BAG3 translocates to the nucleus upon heat stress. • BAG3 interacts with HSF1 and co-localizes to the nucleus. • BAG3 is a key regulator for HSF1 nuclear shuttling

  12. BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon heat stress

    Jin, Young-Hee [Department of Biochemistry, Dongguk University College of Oriental Medicine, Gyeongju 780-714 (Korea, Republic of); Ahn, Sang-Gun [Department of Pathology, Chosun University College of Dentistry, Gwangju 501-759 (Korea, Republic of); Kim, Soo-A., E-mail: ksooa@dongguk.ac.kr [Department of Biochemistry, Dongguk University College of Oriental Medicine, Gyeongju 780-714 (Korea, Republic of)

    2015-08-21

    Bcl2-associated athoanogene (BAG) 3 is a member of the co-chaperone BAG family. It is induced by stressful stimuli such as heat shock and heavy metals, and it regulates cellular adaptive responses against stressful conditions. In this study, we identified a novel role for BAG3 in regulating the nuclear shuttling of HSF1 during heat stress. The expression level of BAG3 was induced by heat stress in HeLa cells. Interestingly, BAG3 rapidly translocalized to the nucleus upon heat stress. Immunoprecipitation assay showed that BAG3 interacts with HSF1 under normal and stressed conditions and co-translocalizes to the nucleus upon heat stress. We also demonstrated that BAG3 interacts with HSF1 via its BAG domain. Over-expression of BAG3 down-regulates the level of nuclear HSF1 by exporting it to the cytoplasm during the recovery period. Depletion of BAG3 using siRNA results in reduced nuclear HSF1 and decreased Hsp70 promoter activity. BAG3 in MEF(hsf1{sup −/−}) cells actively translocalizes to the nucleus upon heat stress suggesting that BAG3 plays a key role in the processing of the nucleocytoplasmic shuttling of HSF1 upon heat stress. - Highlights: • The expression level of BAG3 is induced by heat stress. • BAG3 translocates to the nucleus upon heat stress. • BAG3 interacts with HSF1 and co-localizes to the nucleus. • BAG3 is a key regulator for HSF1 nuclear shuttling.

  13. HSF1-deficiency affects gait coordination and cerebellar calbindin levels.

    Ingenwerth, Marc; Estrada, Veronica; Stahr, Anna; Müller, Hans Werner; von Gall, Charlotte

    2016-09-01

    Heat shock proteins (HSPs) play an important role in cell homeostasis and protect against cell damage. They were previously identified as key players in different ataxia models. HSF1 is the main transcription factor for HSP activation. HSF1-deficient mice (HSF1-/-) are known to have deficiencies in motor control test. However, little is known about effects of HSF1-deficiency on locomotor, especially gait, coordination. Therefore, we compared HSF-deficient (HSF1-/-) mice and wildtype littermates using an automated gait analysis system for objective assessment of gait coordination. We found significant changes in gait parameters of HSF1-/- mice reminiscent of cerebellar ataxia. Immunohistochemical analyses of a cerebellum revealed co-localization of HSF1 and calbindin in Purkinje cells. Therefore, we tested the hypothesis of a potential interconnection between HSF1 and calbindin in Purkinje cells. Calbindin levels were analyzed qualitatively and quantitatively by immunohistochemistry and immunoblotting, respectively. While quantitative PCR revealed no differences in calbindin mRNA levels between HSF1+/+ and HSF1-/- mice, calbindin protein levels, however, were significantly decreased in a cerebellum of HSF1-/- mice. A pathway analysis supports the hypothesis of an interconnection between HSF1 and calbindin. In summary, the targeted deletion of HSF1 results in changes of locomotor function associated with changes in cerebellar calbindin protein levels. These findings suggest a role of HSF1 in regular Purkinje cell calcium homeostasis. PMID:27173427

  14. Cloning and sequence analysis of hsf, an outer membrane protein gene of Pasteurella multocida serotype B:2

    A. Priyadarshini

    2014-12-01

    Full Text Available Aim: The present study was undertaken to clone, sequence and analyze the hsf, an outer membrane protein gene of Pasteurella multocida serotype B:2 Materials and Methods: hsf gene was amplified from genomic DNA of P. multocida. Polymerase chain reaction (PCR product was cloned in pET-32a vector and was characterized. hsf gene was sequenced, analyzed and phylogenetic tree was constructed taking sequences of other strains. Results: Amplicon size was found to be 785 bp. Recombinant got characterized through colony PCR and restriction enzyme analysis. Conclusion: hsf gene of P. multocida serotype B is similar to serotype A, but different from serotype D. Further work is needed to evaluate role of Hsf protein in protection studies and to study the antigenic properties of this recombinant protein as a candidate for vaccine.

  15. Targeting HSF1 in Cancer Therapy%靶向HSF1在肿瘤治疗中的作用

    吴谓; 刘飞飞; 刘颖; 张继虹

    2016-01-01

    Recently, many heat shock protein 90 inhibitors have been used against cancers in clinical trials. Discovery of these inhibitors is a milestone for cancer treatment, with more therapeu-tic programs for cancers developed. Cancer cells are more dependent on the function of highly con-served HSF1, which, as a transcript factor, can promote the transcription and expression of HSF. HSF1 is essential for the initial and development of cancers, which can regulate the abnormal sig-ning in cancer cells, inhibit the aneuploidy increased by cell mitosis and apoptosis of cancer cells and promote the metastasis and metabolism of cancer cells. With the development and application of screening methods for small molecule drugs, compounds targeting HSF1 have been reported, inclu-ding quercetin and triptolide. Here, we review the advances in the studies on HSF1-targeting drugs and elucidate the characteristics and mechanism of this type of drugs.%目前许多热休克蛋白90抑制剂已经用于抗癌的临床试验,这些抑制剂的产生是肿瘤治疗的里程碑,为癌症治疗探索出更多的新方法。高度保守的热休克转录因子1(heat shock factor 1, HSF1)作为转录因子促进热休克蛋白基因的转录和表达,肿瘤细胞比正常细胞更依赖其功能, HSF1对肿瘤的起始和维持是必需的:调控肿瘤细胞异常信号,抑制有丝分裂增加基因组非整倍性,抑制肿瘤细胞发生凋亡和促进肿瘤细胞转移和代谢等。随着很多小分子药物筛选方法不断的发现和运用,目前已有部分以HSF1为靶点的化合物研究报道,主要有槲皮素和雷公藤内酯抑制HSF1,同时减少热休克反应。文章综述了以HSF1为靶点的药物的研究前沿,并分别阐述了这类药物作用特点和机制。

  16. Deteriorated stress response in stationary-phase yeast: Sir2 and Yap1 are essential for Hsf1 activation by heat shock and oxidative stress, respectively.

    Inbal Nussbaum

    Full Text Available Stationary-phase cultures have been used as an important model of aging, a complex process involving multiple pathways and signaling networks. However, the molecular processes underlying stress response of non-dividing cells are poorly understood, although deteriorated stress response is one of the hallmarks of aging. The budding yeast Saccharomyces cerevisiae is a valuable model organism to study the genetics of aging, because yeast ages within days and are amenable to genetic manipulations. As a unicellular organism, yeast has evolved robust systems to respond to environmental challenges. This response is orchestrated largely by the conserved transcription factor Hsf1, which in S. cerevisiae regulates expression of multiple genes in response to diverse stresses. Here we demonstrate that Hsf1 response to heat shock and oxidative stress deteriorates during yeast transition from exponential growth to stationary-phase, whereas Hsf1 activation by glucose starvation is maintained. Overexpressing Hsf1 does not significantly improve heat shock response, indicating that Hsf1 dwindling is not the major cause for Hsf1 attenuated response in stationary-phase yeast. Rather, factors that participate in Hsf1 activation appear to be compromised. We uncover two factors, Yap1 and Sir2, which discretely function in Hsf1 activation by oxidative stress and heat shock. In Δyap1 mutant, Hsf1 does not respond to oxidative stress, while in Δsir2 mutant, Hsf1 does not respond to heat shock. Moreover, excess Sir2 mimics the heat shock response. This role of the NAD+-dependent Sir2 is supported by our finding that supplementing NAD+ precursors improves Hsf1 heat shock response in stationary-phase yeast, especially when combined with expression of excess Sir2. Finally, the combination of excess Hsf1, excess Sir2 and NAD+ precursors rejuvenates the heat shock response.

  17. Enhanced antitumoral efficacy and immune response following conditionally replicative adenovirus containing constitutive HSF1 delivery to rodent tumors

    Fan Rong

    2012-05-01

    Full Text Available Abstract Background Oncolytic adenoviruses are promising as anticancer agents but have limited clinical responses. Our previous study showed that heat shock transcription factor 1 (HSF1 overexpression could increase the anti-tumor efficacy of E1B55kD deleted oncolytic adenovirus through increasing the viral burst. Due to the important roles of heat shock proteins (HSPs in eliciting innate and adaptive immunity, we reasoned that besides increasing the viral burst, HSF1 may also play a role in increasing tumor specific immune response. Methods In the present study, intra-dermal murine models of melanoma (B16 and colorectal carcinoma (CT26 were treated with E1B55kD deleted oncolytic adenovirus Adel55 or Adel55 incorporated with cHSF1, HSF1i, HSP70, or HSP90 by intra-tumoral injection. Tumors were surgically excised 72 h post injection and animals were analyzed for tumor resistance and survival rate. Results Approximately 95% of animals in the Adel55-cHSF1 treated group showed sustained resistance upon re-challenge with autologous tumor cells, but not in PBS, Adel55, or Adel55-HSF1i treated groups. Only 50–65% animals in the Adel55-HSP70 and Adel55-HSP90 treated group showed tumor resistance. Tumor resistance was associated with development of tumor type specific cellular immune responses. Adel55-cHSF1 treatment also showed higher efficacy in diminishing progression of the secondary tumor focus than Adel55-HSP70 or Adel55-HSP90 treatment. Conclusions Besides by increasing its burst in tumor cells, cHSF1 could also augment the potential of E1B55kD deleted oncolytic adenovirus by increasing the tumor-specific immune response, which is beneficial to prevent tumor recurrence. cHSF1 is a better gene for neoadjuvant immunotherapy than other heat shock protein genes.

  18. Overexpression of Heat Shock Factor Gene HsfA3 Increases Galactinol Levels and Oxidative Stress Tolerance in Arabidopsis.

    Song, Chieun; Chung, Woo Sik; Lim, Chae Oh

    2016-06-30

    Heat shock factors (Hsfs) are central regulators of abiotic stress responses, especially heat stress responses, in plants. In the current study, we characterized the activity of the Hsf gene HsfA3 in Arabidopsis under oxidative stress conditions. HsfA3 transcription in seedlings was induced by reactive oxygen species (ROS), exogenous hydrogen peroxide (H2O2), and an endogenous H2O2 propagator, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB). HsfA3-overexpressing transgenic plants exhibited increased oxidative stress tolerance compared to untransformed wild-type plants (WT), as revealed by changes in fresh weight, chlorophyll fluorescence, and ion leakage under light conditions. The expression of several genes encoding galactinol synthase (GolS), a key enzyme in the biosynthesis of raffinose family oligosaccharides (RFOs), which function as antioxidants in plant cells, was induced in HsfA3 overexpressors. In addition, galactinol levels were higher in HsfA3 overexpressors than in WT under unstressed conditions. In transient transactivation assays using Arabidopsis leaf protoplasts, HsfA3 activated the transcription of a reporter gene driven by the GolS1 or GolS2 promoter. Electrophoretic mobility shift assays showed that GolS1 and GolS2 are directly regulated by HsfA3. Taken together, these findings provide evidence that GolS1 and GolS2 are directly regulated by HsfA3 and that GolS enzymes play an important role in improving oxidative stress tolerance by increasing galactinol biosynthesis in Arabidopsis. PMID:27109422

  19. Molecular cloning of hsf1 and hsbp1 cDNAs, and the expression of hsf1, hsbp1 and hsp70 under heat stress in the sea cucumber Apostichopus japonicus.

    Xu, Dongxue; Sun, Lina; Liu, Shilin; Zhang, Libin; Yang, Hongsheng

    2016-08-01

    The heat shock response (HSR) is known for the elevated synthesis of heat shock proteins (HSPs) under heat stress, which is mediated primarily by heat shock factor 1 (HSF1). Heat shock factor binding protein 1 (HSBP1) and feedback control of heat shock protein 70 (HSP70) are major regulators of the activity of HSF1. We obtained full-length cDNA of genes hsf1 and hsbp1 in the sea cucumber Apostichopus japonicus, which are the second available for echinoderm (after Strongylocentrotus purpuratus), and the first available for holothurian. The full-length cDNA of hsf1 was 2208bp, containing a 1326bp open reading frame encoding 441 amino acids. The full-length cDNA of hsbp1 was 2850bp, containing a 225bp open reading frame encoding 74 amino acids. The similarities of A. japonicus HSF1 with other species are low, and much higher similarity identities of A. japonicus HSBP1 were shared. Phylogenetic trees showed that A. japonicus HSF1 and HSBP1 were clustered with sequences from S. purpuratus, and fell into distinct clades with sequences from mollusca, arthropoda and vertebrata. Analysis by real-time PCR showed hsf1 and hsbp1 mRNA was expressed constitutively in all tissues examined. The expression of hsf1, hsbp1 and hsp70 in the intestine at 26°C was time-dependent. The results of this study might provide new insights into the regulation of heat shock response in this species. PMID:26952354

  20. Does the ecological study of managed habitats constitute "real" ecology?

    Gábor L. L(O)VEI

    2011-01-01

    Ecology is not a very "old" science,with about one hundred years of history.In the early period,the general attitude was that the study of undisturbed ecological systems will provide us with clues of how the world is organised.To understand this world,we should study the regions,ecosystems,habitats that are still in their undisturbed condition,far from settlements,are uncultivated,and unspoilt.From these,we can form a picture how things should be.We can then use this knowledge to wisely manage other habitats,more under the influence

  1. Analysis list: Hsf1 [Chip-atlas[Archive

    Full Text Available Hsf1 Gonad,Neural + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Hsf1....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Hsf1.5.tsv http://dbarchive.biosciencedbc.jp/kyu...shu-u/mm9/target/Hsf1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Hsf1.Gonad.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/colo/Hsf1.Neural.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/colo/Gonad.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Neural.gml ...

  2. Molecular evolution and expression divergence of the Populus euphratica Hsf genes provide insight into the stress acclimation of desert poplar

    Zhang, Jin; Jia, Huixia; Li, Jianbo; Li, Yu; Lu, Mengzhu; Hu, Jianjun

    2016-01-01

    Heat shock transcription factor (Hsf) family is one of the most important regulators in the plant kingdom. Hsf has been demonstrated to be involved in various processes associated with plant growth, development as well as in response to hormone and abiotic stresses. In this study, we carried out a comprehensive analysis of Hsf family in desert poplar, Populus euphratica. Total of 32 genes encoding Hsf were identified and they were classified into three main classes (A, B, and C). Gene structure and conserved motif analyses indicated that the members in each class were relatively conserved. Total of 10 paralogous pairs were identified in PeuHsf family, in which nine pairs were generated by whole genome duplication events. Ka/Ks analysis showed that PeuHsfs underwent purifying selection pressure. In addition, various cis-acting elements involved in hormone and stress responses located in the promoter regions of PeuHsfs. Gene expression analysis indicated that several PeuHsfs were tissue-specific expression. Compared to Arabidopsis, more PeuHsf genes were significantly induced by heat, drought, and salt stresses (21, 19, and 22 PeuHsfs, respectively). Our findings are helpful in understanding the distinguished adaptability of P. euphratica to extreme environment and providing a basis for functional analysis of PeuHsfs in the future. PMID:27425424

  3. Molecular evolution and expression divergence of the Populus euphratica Hsf genes provide insight into the stress acclimation of desert poplar.

    Zhang, Jin; Jia, Huixia; Li, Jianbo; Li, Yu; Lu, Mengzhu; Hu, Jianjun

    2016-01-01

    Heat shock transcription factor (Hsf) family is one of the most important regulators in the plant kingdom. Hsf has been demonstrated to be involved in various processes associated with plant growth, development as well as in response to hormone and abiotic stresses. In this study, we carried out a comprehensive analysis of Hsf family in desert poplar, Populus euphratica. Total of 32 genes encoding Hsf were identified and they were classified into three main classes (A, B, and C). Gene structure and conserved motif analyses indicated that the members in each class were relatively conserved. Total of 10 paralogous pairs were identified in PeuHsf family, in which nine pairs were generated by whole genome duplication events. Ka/Ks analysis showed that PeuHsfs underwent purifying selection pressure. In addition, various cis-acting elements involved in hormone and stress responses located in the promoter regions of PeuHsfs. Gene expression analysis indicated that several PeuHsfs were tissue-specific expression. Compared to Arabidopsis, more PeuHsf genes were significantly induced by heat, drought, and salt stresses (21, 19, and 22 PeuHsfs, respectively). Our findings are helpful in understanding the distinguished adaptability of P. euphratica to extreme environment and providing a basis for functional analysis of PeuHsfs in the future. PMID:27425424

  4. [Ecological studies in environmental health: Beyond epidemiology].

    Blanco-Becerra, Luis C; Pinzón-Flórez, Carlos E; Idrovo, Álvaro J

    2015-08-01

    Ecological studies provide important and frequent sources of evidence of environmental health, since their unit of analysis is populations. This review summarizes the foundations of ecological studies with the premise that they can be performed using quantitative, qualitative or mixed methods. It presents the logic behind their design, their role in exploring causality, the variables and categories of analysis and the design principles and techniques used to collect data. Examples of ecological studies performed in Latin America are then presented, as well as some common methodological problems and options to address them. Lastly, the relevance of quantitative and qualitative ecological studies to environmental health as a way to overcome the dominance of conceptual and methodological individualism is highlighted, though ecological studies alone do not suffice for studying population health. PMID:26535754

  5. Analysis of Genetic Polymorphisms of HSF1 and HSBP1 Gene in Holstein Ox%荷斯坦种公牛HSF1和HSBP1基因多态性分析

    王延久; 李建斌; 王长法; 鞠志花; 黄金明; 李荣岭; 仲跻峰; 何剑斌; 李秋玲

    2011-01-01

    [ Objective] The aim was to study the genetic polymorphisms of HSF1 and HSBP1 gene in Holstein ox. [ Method] DNA direct sequencing was used for SNPs loci scanning,and CRS-PCR and PCR-RFLP method were used for genotyping of four SNPs in 162 Holstein ox,so as to analyze the polymorphism of HSF1 and HSBP1 gene. [ Result] The scanning results showed that a novel SNP was discovered at 1451 (G/T)in HSF1 gene and three novel SNPs of 324(G/C) ,589(C/T) ,651(C/G) were discovered in the second intron of HSBP1 gene. The polymorphism of HSF1 and HSBP1 gene analysis results showed that,for HSF1 ,the frequency of the AA genotype was higher and A allele was the dominant allele. While for HSBF1 ,AB,AA and BB appeared more frequently,dominant allele at 589 (C/T) was A, and that at 324 (G/C) and 651 (C/G)was B. Chi-square test indicated that 1451(G/T) polymorphic site in the HSF1 gene and 589(C/T) polymorphic site in HSBP1 gene were met Hardy-Weinberg equilibrium in Holstein (P > 0.05 ), while 324 (G/C) and 651 (C/G) polymorphicsites in the HSBP1 gene were not meet Hardy-Weinberg equilibrium (P < 0. 05). [ Conclusion ] The study can provide trial basis for deeply researching the function of HSF1 and HSBP1 gene.%[目的]研究荷斯坦种公牛HSF1和HSBP1基因多态性.[方法]通过DNA测序技术对牛HSF1和HSBP1基因进行SNPs位点扫描,利用CRS-PCR和PCR-RFLP方法对4个单核苷酸多态性(SNPs)进行基因型分型,分析162头荷斯坦种公牛HSF1和HSBP1基因的多态性.[结果]扫描结果表明,在HSF1基因1451(G/T)处发现1个新SNP.在HSBP1基因第2内含子上发现3个新SNPs,分别为324(G/C)、589(C/T)和651(C/G).多态性分析结果表明,HSF1基因1451(G/T)住点的SNP的AA基因型频率最高,优势等住基因为A,而HSBP1基因3个SNP频率最高的基因型分别为AB、AA和BB,优势等位基因589(C/T)为A,而324(G/C)和651(C/G)均为B.χ适合性检验表明,HSF1基因1451(G/T)位点在荷斯坦种公牛群体中已

  6. Comparison of Copy Number of HSF Genes in Two Buffalo Genomes.

    Lal, Shardul Vikram; Mukherjee, Ayan; Brahma, Biswajit; Gohain, Moloya; Patra, Mahesh Chandra; Saini, Sushil Kumar; Mishra, Purushottam; Ahlawat, Sonika; Upadhyaya, Ramesh C; Datta, Tirtha K; De, Sachinandan

    2016-07-01

    The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations follow Mendelian pattern of inheritance, identification and distribution of these variants between populations can be used to infer the evolutionary history of the species. In this study, we have examined the absolute copy number of four Heat shock factor genes viz. HSF-1, 2, 4, and 5 in two different breeds of buffalo species using real-time PCR. Here, we report that the absolute copy number of HSF2 varies between the two breeds. In contrast no significant difference was observed in the copy number for HSF-1, 4, and 5 between the two breeds. Our results provide evidence for the presence of breed specific differences in HSF2 genomic copy number. This seems to be the first step in delineating the genetic factors underlying environmental adaptation between the two breeds. Nevertheless, a more detailed study is needed to characterize the functional consequence of this variation. PMID:26953680

  7. HSF1 transcriptional activity mediates alcohol induction of Vamp2 expression and GABA release

    Florence P. Varodayan

    2013-12-01

    Full Text Available Many central synapses are highly sensitive to alcohol, and it is now accepted that short-term alterations in synaptic function may lead to longer term changes in circuit function. The regulation of postsynaptic receptors by alcohol has been well studied, but the mechanisms underlying the effects of alcohol on the presynaptic terminal are relatively unexplored. To identify a pathway by which alcohol regulates neurotransmitter release, we recently investigated the mechanism by which ethanol induces the Vamp2 gene, but not Vamp1, in mouse primary cortical cultures. These two genes encode isoforms of synaptobrevin, a vesicular soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE protein required for synaptic vesicle fusion. We found that alcohol activates the transcription factor heat shock factor 1 (HSF1 to induce Vamp2 gene expression, while Vamp1 mRNA levels remain unaffected. As the Vamp2 gene encodes a SNARE protein, we then investigated whether ethanol exposure and HSF1 transcriptional activity alter neurotransmitter release using electrophysiology. We found that alcohol increased the frequency of γ-aminobutyric acid (GABA-mediated miniature IPSCs via HSF1, but had no effect on mEPSCs. Overall, these data indicate that alcohol induces HSF1 transcriptional activity to trigger a specific coordinated adaptation in GABAergic presynaptic terminals. This mechanism could explain some of the changes in synaptic function that occur soon after alcohol exposure, and may underlie some of the more enduring effects of chronic alcohol intake on local circuit function.

  8. Heat Shock Factors HsfB 1 and HsfB2b Are Involved in the Regulation of Pdfl.2 Expression and Pathogen Resistance in Arabidopsis

    Mukesh Kumar; Wolfgang Busch; Hannah Birke; Birgit Kemmerling; Thorsten N(U)rnberger; Friedrich Sch(o)ffl

    2009-01-01

    In order to assess the functional roles of heat stress-induced class B-heat shock factors in Arabidopsis, we investigated T-DNA knockout mutants of AtHsfB1 and AtHsfB2b. Micorarray analysis of double knockout hsfBl/hsfB2b plants revealed as strong an up-regulation of the basal mRNA-levels of the defensin genes Pdfl.2a/b in mutant plants.The Pdfexpression was further enhanced by jasmonic acid treatment or infection with the necrotrophic fungus Alternaria brassicicola. The single mutant hsfB2b and the double mutant hsfB1/B2b were significantly improved in disease resistance after A. brassicicola infection. There was no indication for a direct interaction of Hsf with the promoter of Pdfl.2, which is devoid of perfect HSE consensus Hsf-binding sequences. However, changes in the formation of late HsfA2-dependent HSE binding were detected in hsfB1/B2b plants. This suggests that HsfB1/B2b may interact with class A-Hsf in regulating the shut-off of the heat shock response. The identification of Pdfgenes as targets of Hsf-dependent negative regulation is the first evidence for an interconnection of Hsf in the regulation of biotic and abiotic responses.

  9. HSF1 is essential for the resistance of zebrafish eye and brain tissues to hypoxia/reperfusion injury.

    Nathan R Tucker

    Full Text Available Ischemia and subsequent reperfusion (IR produces injury to brain, eye and other tissues, contributing to the progression of important clinical pathologies. The response of cells to IR involves activation of several signaling pathways including those activating hypoxia and heat shock responsive transcription factors. However, specific roles of these responses in limiting cell damage and preventing cell death after IR have not been fully elucidated. Here, we have examined the role of heat shock factor 1 (HSF1 in the response of zebrafish embryos to hypoxia and subsequent return to normoxic conditions (HR as a model for IR. Heat shock preconditioning elevated heat shock protein expression and protected zebrafish embryo eye and brain tissues against HR-induced apoptosis. These effects were inhibited by translational suppression of HSF1 expression. Reduced expression of HSF1 also increased cell death in brain and eye tissues of embryos subjected to hypoxia and reperfusion without prior heat shock. Surprisingly, reduced expression of HSF1 had only a modest effect on hypoxia-induced expression of Hsp70 and no effect on hypoxia-induced expression of Hsp27. These results establish the zebrafish embryo as a model for the study of ischemic injury in the brain and eye and reveal a critical role for HSF1 in the response of these tissues to HR. Our results also uncouple the role of HSF1 expression from that of Hsp27, a well characterized heat shock protein considered essential for cell survival after hypoxia. Alternative roles for HSF1 are considered.

  10. Study on ecological migration in Xinjiang, China

    Abliz Yusup; Osman Niyaz

    2006-01-01

    This paper expounds on the meaning of migration and the status quo of studies at home and abroad on migration and ecological migration. It focuses on the discussion of the necessity of ecological migration in Xinjiang, and the problems faced by the ecological emigrants, such as the lack of water, the serious land salination and basification,sand storms, and the adaptation to the environment. Besides, it probes into the problems existing in the ecological migration, including the unscientific design of the moving project, the defects of the reclamation work, and the irrational treatment of the relation between ecological migration and regional development. Based on this, we put forward some suggestions about and countermeasures against those problems.

  11. 热休克因子1对FasL的调控作用%The transcription regulation of HSF1 on FasL

    鄂顺梅; 陈茶; 曾建明; 邹江英

    2012-01-01

    目的 研究人热休克因子1( HSF1)对Fas配体(FasL)启动子转录活性的影响.方法 用在线启动子分析软件分析FasL启动子区转录因子结合位点;凝胶阻滞实验(EMSA)研究内源性HSF1与FasL启动子区的热休克元件(HSE)结合能力;将组成型活化的HSF1突变体与FasL启动子表达载体FasL-luc共同转染HeLa细胞,采用双荧光素酶报告基因检测系统,检测荧光索酶活性.结果 在FasL启动子区发现HSE核心序列(nGAAnnTTCn),HSF1可以与该HSE体外结合;荧光素酶活性测定发现HSF1明显上调FasL-luc转录活性,突变该HSE,则转录激活作用消失.结论 HSF1对FasL具有转录调控作用.%Objective To explore the transcription regulation and molecular mechanism of heat shock factor 1(HSF1) on Fas ligand of human and mouse. Methods The conserved region within FasL promoter upstream of the translational start site that contains heat shock element (HSE) was analyzed. DNA binding activity of HSF1 on the HSE of FasL promoter was studied by electrophoretic mobility shift assay (EMSA).HeLa cells transiently transfected with pcDNA or pcDNA-HSFld203-315 and pGL3-FasL.Dual-luciferase reporter assay was used to study the transcriptional activity of HSF1 among different groups. Results HSE consists of one perfect nGAAn pentamers, housed within the proximal region of the FasL promoter. HSF1 can directly bind to this element and upregulated transcriptional activity of FasL promoter. The mutation of HSE can abrogate the ability of HSF1 to bind and abrogate the promoter activity. Conclusion HSF1 induce transactivation activity of FasL.

  12. Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice

    Ai-Ling Liu

    2013-01-01

    Full Text Available Heat shock proteins play an important role in plant stresstolerance and are mainly regulated by heat shock transcriptionfactors (Hsfs. In this study, we generated transgenic riceover-expressing OsHsfA7 and carried out morphologicalobservation and stress tolerance assays. Transgenic plantsexhibited less, shorter lateral roots and root hair. Under salttreatment, over-expressing OsHsfA7 rice showed alleviativeappearance of damage symptoms and higher survival rate, leafelectrical conductivity and malondialdehyde content of transgenicplants were lower than those of wild type plants. Meanwhile,transgenic rice seedlings restored normal growth but wild typeplants could not be rescued after drought and re-wateringtreatment. These findings indicate that over-expression ofOsHsfA7 gene can increase tolerance to salt and drought stressesin rice seedlings. [BMB Reports 2013; 46(1: 31-36

  13. Molecular cloning and expression of a human heat shock factor, HSF1

    Rabindran, S.K.; Giorgi, G.; Clos, J.; Wu, C. (National Institutes of Health, Bethesda, MD (United States))

    1991-08-15

    Human cells respond to heat stress by inducing the binding of a preexisting transcriptional activator (heat shock factor, HSF) to DNA. The authors isolated recombinant DNA clones for a human cDNA fragment. The human HSF1 probe was produced by the PCR with primers deduced from conserved amino acids in the Drosophila and yeast HSF sequences. The human HSF1 mRNA is constitutively expressed in HeLa cells under nonshock conditions and encodes a protein with four conserved leucine zipper motifs. Like its counterpart in Drosophila, human HSF1 produced in Escherichia coli in the absence of heat shock is active as a DNA binding transcription factor, suggesting that the intrinsic activity of HSF is under negative control in human cells. Surprisingly, an independently isolated human HSF clone, HSF2, is related to but significantly different from HSF.

  14. Synchronization of circadian Per2 rhythms and HSF1-BMAL1:CLOCK interaction in mouse fibroblasts after short-term heat shock pulse.

    Teruya Tamaru

    Full Text Available Circadian rhythms are the general physiological processes of adaptation to daily environmental changes, such as the temperature cycle. A change in temperature is a resetting cue for mammalian circadian oscillators, which are possibly regulated by the heat shock (HS pathway. The HS response (HSR is a universal process that provides protection against stressful conditions, which promote protein-denaturation. Heat shock factor 1 (HSF1 is essential for HSR. In the study presented here, we investigated whether a short-term HS pulse can reset circadian rhythms. Circadian Per2 rhythm and HSF1-mediated gene expression were monitored by a real-time bioluminescence assay for mPer2 promoter-driven luciferase and HS element (HSE; HSF1-binding site-driven luciferase activity, respectively. By an optimal duration HS pulse (43°C for approximately 30 minutes, circadian Per2 rhythm was observed in the whole mouse fibroblast culture, probably indicating the synchronization of the phases of each cell. This rhythm was preceded by an acute elevation in mPer2 and HSF1-mediated gene expression. Mutations in the two predicted HSE sites adjacent (one of them proximally to the E-box in the mPer2 promoter dramatically abolished circadian mPer2 rhythm. Circadian Per2 gene/protein expression was not observed in HSF1-deficient cells. These findings demonstrate that HSF1 is essential to the synchronization of circadian rhythms by the HS pulse. Importantly, the interaction between HSF1 and BMAL1:CLOCK heterodimer, a central circadian transcription factor, was observed after the HS pulse. These findings reveal that even a short-term HS pulse can reset circadian rhythms and cause the HSF1-BMAL1:CLOCK interaction, suggesting the pivotal role of crosstalk between the mammalian circadian and HSR systems.

  15. Heterotypic Signals from Neural HSF-1 Separate Thermotolerance from Longevity.

    Douglas, Peter M; Baird, Nathan A; Simic, Milos S; Uhlein, Sarah; McCormick, Mark A; Wolff, Suzanne C; Kennedy, Brian K; Dillin, Andrew

    2015-08-18

    Integrating stress responses across tissues is essential for the survival of multicellular organisms. The metazoan nervous system can sense protein-misfolding stress arising in different subcellular compartments and initiate cytoprotective transcriptional responses in the periphery. Several subcellular compartments possess a homotypic signal whereby the respective compartment relies on a single signaling mechanism to convey information within the affected cell to the same stress-responsive pathway in peripheral tissues. In contrast, we find that the heat shock transcription factor, HSF-1, specifies its mode of transcellular protection via two distinct signaling pathways. Upon thermal stress, neural HSF-1 primes peripheral tissues through the thermosensory neural circuit to mount a heat shock response. Independent of this thermosensory circuit, neural HSF-1 activates the FOXO transcription factor, DAF-16, in the periphery and prolongs lifespan. Thus a single transcription factor can coordinate different stress response pathways to specify its mode of protection against changing environmental conditions. PMID:26257177

  16. Heterotypic Signals from Neural HSF-1 Separate Thermotolerance from Longevity

    Peter M. Douglas

    2015-08-01

    Full Text Available Integrating stress responses across tissues is essential for the survival of multicellular organisms. The metazoan nervous system can sense protein-misfolding stress arising in different subcellular compartments and initiate cytoprotective transcriptional responses in the periphery. Several subcellular compartments possess a homotypic signal whereby the respective compartment relies on a single signaling mechanism to convey information within the affected cell to the same stress-responsive pathway in peripheral tissues. In contrast, we find that the heat shock transcription factor, HSF-1, specifies its mode of transcellular protection via two distinct signaling pathways. Upon thermal stress, neural HSF-1 primes peripheral tissues through the thermosensory neural circuit to mount a heat shock response. Independent of this thermosensory circuit, neural HSF-1 activates the FOXO transcription factor, DAF-16, in the periphery and prolongs lifespan. Thus a single transcription factor can coordinate different stress response pathways to specify its mode of protection against changing environmental conditions.

  17. Protein refolding in peroxisomes is dependent upon an HSF1-regulated function

    Heldens, Lonneke; van Genesen, Siebe T.; Hanssen, Lars L. P.; Hageman, Jurre; Kampinga, Harm H.; Lubsen, Nicolette H.

    2012-01-01

    Post-heat shock refolding of luciferase requires chaperones. Expression of a dominant negative HSF1 mutant (dnHSF1), which among other effects depletes cells of HSF1-regulated chaperones, blocked post-heat shock refolding of luciferase targeted to the cytoplasm, nucleus, or peroxisomes, while refold

  18. Thermal discharges and ecological studies - an overview

    Thermal ecological studies (TES) formulated and funded by BRNS are fine examples of coordinated research program in our country. There were at least eight different research laboratories with various backgrounds involved in these studies with a commitment to study the effect of thermal discharge into water bodies scientifically. BRNS has already underlined the concern of the people living within the vicinity of the power plants on their livelihood which depends on fish catch etc., and the stipulations laid down by statutory bodies for the temperature rise due to thermal discharge to be viewed in a global perspective

  19. Hsf1 activation inhibits rapamycin resistance and TOR signaling in yeast revealed by combined proteomic and genetic analysis.

    Sricharan Bandhakavi

    Full Text Available TOR kinases integrate environmental and nutritional signals to regulate cell growth in eukaryotic organisms. Here, we describe results from a study combining quantitative proteomics and comparative expression analysis in the budding yeast, S. cerevisiae, to gain insights into TOR function and regulation. We profiled protein abundance changes under conditions of TOR inhibition by rapamycin treatment, and compared this data to existing expression information for corresponding gene products measured under a variety of conditions in yeast. Among proteins showing abundance changes upon rapamycin treatment, almost 90% of them demonstrated homodirectional (i.e., in similar direction transcriptomic changes under conditions of heat/oxidative stress. Because the known downstream responses regulated by Tor1/2 did not fully explain the extent of overlap between these two conditions, we tested for novel connections between the major regulators of heat/oxidative stress response and the TOR pathway. Specifically, we hypothesized that activation of regulator(s of heat/oxidative stress responses phenocopied TOR inhibition and sought to identify these putative TOR inhibitor(s. Among the stress regulators tested, we found that cells (hsf1-R206S, F256S and ssa1-3 ssa2-2 constitutively activated for heat shock transcription factor 1, Hsf1, inhibited rapamycin resistance. Further analysis of the hsf1-R206S, F256S allele revealed that these cells also displayed multiple phenotypes consistent with reduced TOR signaling. Among the multiple Hsf1 targets elevated in hsf1-R206S, F256S cells, deletion of PIR3 and YRO2 suppressed the TOR-regulated phenotypes. In contrast to our observations in cells activated for Hsf1, constitutive activation of other regulators of heat/oxidative stress responses, such as Msn2/4 and Hyr1, did not inhibit TOR signaling. Thus, we propose that activated Hsf1 inhibits rapamycin resistance and TOR signaling via elevated expression of specific

  20. Study of Teacher Development Based on the Ecological Perspective

    Liu Bo; Xiao Qianyin; Xiong Feng

    2014-01-01

    Based on the understanding of the ecology background, the ecological system of Teacher Development (TD) is constructed and the influential factors, such as the natural, social environment, regulatory environment and the physiological and psychological environment, which have an effect on TD, are analyzed in the present study. Besides, with the combination of the ecological characteristics of TD, The sustainable model for TD is proposed. In accordance with the ecological principles of TD and w...

  1. Ecological studies on Prochlorococcus in China seas

    2002-01-01

    Prochlorococcus, a tiny oxygenic photosynthetic picoplankton with unique pigment composition, has been found to be ubiquitous and abundant in the world oceans, and has been recognized to be closely related to living resources and environmental issues. It has attracted the interest of marine biologists since its discovery, and field data on it over global oceans have accumulated rapidly in the past 10 years. In China, we have studied Prochlorococcus for 8 years, achieving a basic ecological understanding. The presence of Prochlorococcus in China seas, marginal seas of the west Pacific, was confirmed, and its distribution patterns were also brought to light. Prochlorococcus is very abundant in the South China Sea and the offshore regions of the East China Sea. It is seasonally present in the southeast part of the Yellow Sea and absent in the Bohai Sea. Temporal and spatial variations of the abundance of Prochlorococcus and their affecting factors, physiological and ecological characteristics of Prochlorococcus and their relationships to the other groups of picoplankton, and the importance of Prochlorococcus in total biomass and possible roles in living resources and environmental problems are discussed. In the future, isolation of different Prochlorococcus strains from the China seas and their physiological characteristics, genetic diversity, phylogenies and gene exploiture, etc. are important issues to be addressed.

  2. Molecular characterization and expression of HSP70, HSF and HSBP genes inOctopus vulgaris during thermal stress

    HONG Jingni; MAO Yong; NIU Sufang; SUN Tiantian; SU Yongquan

    2015-01-01

    Temperature is an important environmental factor that affects the growth and survival ofOctopus vulgaris, the common octopus. To understand the protective mechanism thatO.vulgaris exhibits under heat stress, we used rapid amplification of cDNA ends (RACE) to obtain full-length sequences of three heat stress response related genes: (1) the heat shock protein 70 (OvHSP70), (2) the heat shock transcription factor (OvHSF) , and (3) the heat shock factor-binding protein (OvHSBP) ofO.vulgaris. The OvHSP70, OvHSF, and OvHSBP proteins contained 2 222 bp, 2 264 bp, 841 bp that encoded for 635, 458 and 90 amino acids, respectively. The results of multiple sequence alignment showed that the amino acid sequences of OvHSP70 were highly conserved with respect to other species. Similarly, the DNA binding domain, the trimerization domain of OvHSF, and the coiled coil region of OvHSBP also had highly conserved regions. The real-time polymerase chain reaction (PCR) results indicated that OvHSP70 was temperature-dependent and time-dependent, showing a positive response to heat stress. On exposure to 28°C and to 30°C, the mRNA expression levels of OvHSF and OvHSBP were higher than those in the control group at 24°C. The mRNA expression of OvHSBP significantly increased with heat treatment at 26°C, while the mRNA expression of OvHSF decreased. The experimental results indicated that the expression of OvHSP70, OvHSF and OvHSBP were all sensitive to heat stress, which suggests that these three genes may play an important role forO.vulgaris in responding to environmental stress. Thus, this study sets a theoretical foundation for further in-depth studies on the molecular protective mechanisms of the heat response inO.vulgaris.

  3. Tomato HsfA1a plays a critical role in plant drought tolerance by activating ATG genes and inducing autophagy

    Wang, Yu; Cai, Shuyu; Yin, Lingling; Kai SHI; Xia, Xiaojian; Zhou, Yanhong; Yu, Jingquan; Zhou, Jie

    2015-01-01

    Autophagy plays critical roles in plant responses to stress. In contrast to the wealth of information concerning the core process of plant autophagosome assembly, our understanding of the regulation of autophagy is limited. In this study, we demonstrated that transcription factor HsfA1a played a critical role in tomato tolerance to drought stress, in part through its positive role in induction of autophagy under drought stress. HsfA1a expression was induced by drought stress. Virus-induced Hs...

  4. Downregulation of chloroplast RPS1 negatively modulates nuclear heat-responsive expression of HsfA2 and its target genes in Arabidopsis.

    Hai-Dong Yu

    Full Text Available Heat stress commonly leads to inhibition of photosynthesis in higher plants. The transcriptional induction of heat stress-responsive genes represents the first line of inducible defense against imbalances in cellular homeostasis. Although heat stress transcription factor HsfA2 and its downstream target genes are well studied, the regulatory mechanisms by which HsfA2 is activated in response to heat stress remain elusive. Here, we show that chloroplast ribosomal protein S1 (RPS1 is a heat-responsive protein and functions in protein biosynthesis in chloroplast. Knockdown of RPS1 expression in the rps1 mutant nearly eliminates the heat stress-activated expression of HsfA2 and its target genes, leading to a considerable loss of heat tolerance. We further confirm the relationship existed between the downregulation of RPS1 expression and the loss of heat tolerance by generating RNA interference-transgenic lines of RPS1. Consistent with the notion that the inhibited activation of HsfA2 in response to heat stress in the rps1 mutant causes heat-susceptibility, we further demonstrate that overexpression of HsfA2 with a viral promoter leads to constitutive expressions of its target genes in the rps1 mutant, which is sufficient to reestablish lost heat tolerance and recovers heat-susceptible thylakoid stability to wild-type levels. Our findings reveal a heat-responsive retrograde pathway in which chloroplast translation capacity is a critical factor in heat-responsive activation of HsfA2 and its target genes required for cellular homeostasis under heat stress. Thus, RPS1 is an essential yet previously unknown determinant involved in retrograde activation of heat stress responses in higher plants.

  5. TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets

    Xue, Gang-ping; Drenth, Janneke; McIntyre, C. Lynne

    2014-01-01

    Heat stress is a significant environmental factor adversely affecting crop yield. Crop adaptation to high-temperature environments requires transcriptional reprogramming of a suite of genes involved in heat stress protection. This study investigated the role of TaHsfA6f, a member of the A6 subclass of heat shock transcription factors, in the regulation of heat stress protection genes in Triticum aestivum (bread wheat), a poorly understood phenomenon in this crop species. Expression analysis s...

  6. Studies on rhizobial ecology using marker genes

    Not only traditional approaches such as serology, antibiotic resistance and plasmid profile analysis but also technologies based on molecular biology have been used for strain identification in studies on microbial ecology. Many methods suffer from certain limitations because they are often laborious and time consuming, requiring analysis of individual nodules, and therefore only a restricted amount of data can be collected. Use of marker genes has become popular, especially when they allow visual identification. The Escherichia coli gusA marker gene has proved to be a highly suitable tool for studying plant-microbe interactions. Nodules containing gusA marked rhizobia can be identified simply by their blue colour, which they produce after incubation in a phosphate buffer containing the substrate X-gluc, but marked bacteria can also be detected on plates or in liquid culture. Colorigenic markers have several advantages, since they are easy to use and it is possible to analyse a large sample size. They are suitable for competition studies of Rhizobium, detecting also double occupancy in nodules, and for studies on motility and survival. Marker genes greatly facilitate screening for successful strains in a specific environment and could be used in future for quality control of inoculants. (author). 30 refs, 3 figs, 1 tab

  7. Cloning, Localization and Expression Analysis of ZmHsf-like Gene in Zea mays

    LI Hui-cong; LI Guo-liang; LIU Zi-hui; ZHANG Hong-mei; ZHANG Yan-min; GUO Xiu-lin

    2014-01-01

    Using homology cloning method, a heat shock transcription factor (Hsf) like gene, ZmHsf-like, was cloned from maize (Zea mays) leaves. Sequence analyses showed that the open reading frame (ORF) of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we ifrstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H2O2 in different degrees, among which the heat shock and ABA worked more efifciently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H2O2-dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H2O2. These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.

  8. Inhibition of heat shock factor1 response enhances PS-341-mediated glioma cell apoptosis%抑制 HSF-1增强 PS-341诱导胶质瘤细胞凋亡

    王宏瑜; 毕云科; 刘耀华; 徐龙庆; 王瑞恒; 赵世光

    2014-01-01

    目的:探索HSF1是否能诱导HSPs的高表达,以及抑制HSF1是否能够增强PS-341诱导胶质瘤细胞凋亡。方法 Western检测HSP70、HSF1的表达,以及JNK的磷酸化。转染siRNA敲除HSF1,胎盘蓝染色及sub-G1检测细胞凋亡。结果胶质瘤细胞中HSP70及HSF1的表达明显高于正常脑组织。敲除HSF1能通过抑制HSP70明显增强PS-341诱导的胶质瘤细凋亡,并能增强及延长JNK通路的活化。在HSF1+/+细胞中, PS-341能够强烈诱导HSP70的表达;而在HSF1-/-细胞中,PS-341诱导并延长了JNK通路的激活。热休克预处理对两种细胞活性都没明显影响,但能明显增强HSF1+/+细胞对抗PS-341诱导凋亡的能力。结论胶质瘤细胞中,HSF1的激活能促进HSPs的表达,进而对抗PS-341诱导的细胞凋亡。抑制HSF-1能增强PS-341诱导胶质瘤细胞凋亡,这有望成为一种新的胶质瘤治疗途径。%Objective Proteasome inhibitor have profound effects on tumor growth and cause cells to undergo apoptosis .PS-341,as an extremely potent and selective proteasome inhibitor , which is the first proteasome inhibitor to be used in clinical practice with the approve of FDA , can induces cell death via JNK pathway in vitro in glioma .However,suppressing proteasome complex by PS-341 may induce activation of heat shock factor 1 ( HSF1 ) , which can induce the expression of heat shock proteins ( HSPs ) to against cellular stress .In this study , we explored whether HSF 1 could induce expression of HSPs and whether inhibition of HSF 1 could enhance cell damage induced by PS-341 in glioma cells.Methods Surgically excised specimens from 30 patients with glioma (gradeⅡ-Ⅳ) and 10 normal brain control specimens obtained at autopsy were collected from the Department of Neurosurgery ,The First Affiliated Hospital of Harbin Medical University , China.The histological characteristics of 30 patients with glioma were established based on World Health

  9. Lubei Ecological Industry Project:A Case Study in China

    Feng Jiutian

    2004-01-01

    Lubei General Company of Enterprise Group, Shangdong Province, set up joint enterprises industrial ecosystem by sharing resources, symbiosis industry and compact structure. As an ecological industry park, the industrial ecosystem has been operated successfully with rich experiment and has become one of ecological industry examples in the world. Based on the case study of Lubei ecological industry project, components of industrial chains,matter recycle and conversion, energy cycle and multilevel utilization, systematic structure and impact factor identification are analyzed and summarized in this paper. The possible extension and development of Lubei ecological industry project in future is brought forward as well.

  10. Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation

    Fuda, Nicholas J.; Mahat, Dig B.; Core, Leighton J.; Guertin, Michael J.

    2016-01-01

    The coordinated regulation of gene expression at the transcriptional level is fundamental to development and homeostasis. Inducible systems are invaluable when studying transcription because the regulatory process can be triggered instantaneously, allowing the tracking of ordered mechanistic events. Here, we use precision run-on sequencing (PRO-seq) to examine the genome-wide heat shock (HS) response in Drosophila and the function of two key transcription factors on the immediate transcription activation or repression of all genes regulated by HS. We identify the primary HS response genes and the rate-limiting steps in the transcription cycle that GAGA-associated factor (GAF) and HS factor (HSF) regulate. We demonstrate that GAF acts upstream of promoter-proximally paused RNA polymerase II (Pol II) formation (likely at the step of chromatin opening) and that GAF-facilitated Pol II pausing is critical for HS activation. In contrast, HSF is dispensable for establishing or maintaining Pol II pausing but is critical for the release of paused Pol II into the gene body at a subset of highly activated genes. Additionally, HSF has no detectable role in the rapid HS repression of thousands of genes. PMID:27492368

  11. Molecular ecology studies of marine Synechococcus

    MA Ying; JIAO Nianzhi

    2004-01-01

    Cyanobacteria of the genus Synechococcus is a dominant component of microbial community in the world's oceans, and is a major contributor to marine primary productivity and thus plays an important role in carbon cycling in the oceans. Besides the ecological importance, the cultivability also made Synechococcus a very special group of marine microorganisms, which has attracted great attention from oceanographers and biologists. Great progress in the physiology, biochemistry and phylogeny of Synechococcus has been made since its discovery. We here review the current status of molecular ecology of marine Synechococcus and give a perspective into the future based on our understanding of the literature and our own work.

  12. Ecological Benefits Evaluation in Ecological Migration Zone Based on Ecological Green Equivalent: A Case Study of Migration Zone in Yanchi County

    Yun; SHI; Muwen; HAN; Zhuzhou; ZHUANG; Chao; Ma; Jin; WU; Xue; MA

    2015-01-01

    With four ecological migration zones in Huamachi Town of Yanchi County in Ningxia Autonomous Region as the object of study,we carry out the evaluation of ecological benefits in ecological migration zone. Using the SPOT satellite remote sensing image in 2008 and UAV aerophotographic image in 2013,we first monitor and analyze the land use change over five years in the study area,and then adopt ecological green equivalent evaluation model for the evaluation of ecological benefits in the ecological migration zone. Studies have shown that:( i) from 2008 to 2013,the ecological green equivalent in the study area was increased and the ecological environment was improved;( ii) the ecological green equivalent in the study area was less than 1 in 2008 and 2013,and ecological environment was still fragile in the migration zone;( iii)the forest coverage rate of the study area was 20% less than the minimum forest coverage rate of the United Nations,but 15% higher than the forest coverage rate of the Ministry of Environmental Protection. There is a large gap between the forest coverage rate based on ecological green equivalent and optimal forest coverage rate,suggesting that the land use still needs to be adjusted in study area,and it is necessary to increase efforts to strengthen ecological restoration and continue to implement forest conservation,returning land for farming to forestry and other measures.

  13. The master regulator of the cellular stress response (HSF1 is critical for orthopoxvirus infection.

    Claire Marie Filone

    2014-02-01

    Full Text Available The genus Orthopoxviridae contains a diverse group of human pathogens including monkeypox, smallpox and vaccinia. These viruses are presumed to be less dependent on host functions than other DNA viruses because they have large genomes and replicate in the cytoplasm, but a detailed understanding of the host factors required by orthopoxviruses is lacking. To address this topic, we performed an unbiased, genome-wide pooled RNAi screen targeting over 17,000 human genes to identify the host factors that support orthopoxvirus infection. We used secondary and tertiary assays to validate our screen results. One of the strongest hits was heat shock factor 1 (HSF1, the ancient master regulator of the cytoprotective heat-shock response. In investigating the behavior of HSF1 during vaccinia infection, we found that HSF1 was phosphorylated, translocated to the nucleus, and increased transcription of HSF1 target genes. Activation of HSF1 was supportive for virus replication, as RNAi knockdown and HSF1 small molecule inhibition prevented orthopoxvirus infection. Consistent with its role as a transcriptional activator, inhibition of several HSF1 targets also blocked vaccinia virus replication. These data show that orthopoxviruses co-opt host transcriptional responses for their own benefit, thereby effectively extending their functional genome to include genes residing within the host DNA. The dependence on HSF1 and its chaperone network offers multiple opportunities for antiviral drug development.

  14. HSF1的抗炎症作用机制

    王玉玺; 敖杰男

    2009-01-01

    目前,已经发现4种热休克转录因子(Heat Shock Transcription Factor,HSF),除HSF3存在于鸟类基因外,其它三种(HSF1、HSF2、HSF4)可见于哺乳动物细胞,不同种类的HSF结构上相似,但功能上存在不同程度的差异:HSF1可以被热应激、氧化应激、化学应激和生理应激等激活,主要介导热休克蛋白(HSPs)合成,HSF2在发育和细胞分化的不同阶段调节热休克基因的表达,HSF4可能阻遏某些蛋白质的表达,其基因突变与白内障的早发形式密切相关。其中HSF1对机体炎症的保护作用除了通过诱导保护性基因表达上调来实现外,还可通过抑制损伤性基因表达下调来实现。

  15. Expression of HSF2 decreases in mitosis to enable stress-inducible transcription and cell survival

    Elsing, Alexandra N.; Aspelin, Camilla; Björk, Johanna K.; Bergman, Heidi A.; Himanen, Samu V.; Kallio, Marko J.; Roos-Mattjus, Pia

    2014-01-01

    Unless mitigated, external and physiological stresses are detrimental for cells, especially in mitosis, resulting in chromosomal missegregation, aneuploidy, or apoptosis. Heat shock proteins (Hsps) maintain protein homeostasis and promote cell survival. Hsps are transcriptionally regulated by heat shock factors (HSFs). Of these, HSF1 is the master regulator and HSF2 modulates Hsp expression by interacting with HSF1. Due to global inhibition of transcription in mitosis, including HSF1-mediated expression of Hsps, mitotic cells are highly vulnerable to stress. Here, we show that cells can counteract transcriptional silencing and protect themselves against proteotoxicity in mitosis. We found that the condensed chromatin of HSF2-deficient cells is accessible for HSF1 and RNA polymerase II, allowing stress-inducible Hsp expression. Consequently, HSF2-deficient cells exposed to acute stress display diminished mitotic errors and have a survival advantage. We also show that HSF2 expression declines during mitosis in several but not all human cell lines, which corresponds to the Hsp70 induction and protection against stress-induced mitotic abnormalities and apoptosis. PMID:25202032

  16. Role of HSF1 Knock-out in Protection of Heat Shock Response Against Endotoxemia%HSF1基因剔除对HSR抗内毒素血症的影响

    陈广文; 王慷慨; 刘瑛; 唐道林; 肖献忠

    2008-01-01

    利用内毒素(LPS)血症小鼠模型,观察HSF1基因剔除对热休克反应(HSR)保护作用的影响.采用腹腔注射LPS建立内毒素血症小鼠模型,HSR采用肛温42℃维持15 min,室温恢复24 h,利用RT-PCR、苏木素-伊红(HE)染色、丙二醛测定以及死亡率,计算和分析重要脏器组织中炎症介质基因的表达、脏器损伤程度及小鼠存活率.注射LPS 15mg/kg 72 h后HSR+LPS(HSF1+/+)组存活率(7/15)显著高于LPS(HSF1+/+)组(0/15)、LPS(HSF1-/-)组(0/14)和HSR+LPS(HSF1-/-)组(0/14),而注射LPS 14 mg/kg 72 h后,LPS(HSF1+/+)组存活率(5/15)显著高于LPS(HSF1-/-)组(0/13)和HSR+LPS(HSF1-/-)组(0/13).在注射LPS 12 h后LPS(HSF1+/+)组、LPS(HSF1-/-)组和HSR+LPS(HSF1-/-)组的心、肺组织丙二醛含量显著升高,但HSR+LPS(HSF1+/+)组不升高.肺组织炎症介质基因IL-IB、IL-6、TNF-α、CCL-2、SOCS3、MCSF、GCSF、IL-15在LPS(HSF1-/-)组和LPS(HSF1+/+)组表达上调,HSR+LPS(HSF1-/-)组除IL-15较低外其他上调更甚,HSR+LPS(HSF1+/+)组除IL-1β和TNF-α较高外其他显著下调.注射LPS后LPS(HSF1+/+)组和LPS(HSF1-/-)组的肺、肝、肾病理形态改变明显,HSR+LPS(HSF1+/+)组改变较轻,HSR+LPS(HSF1-/-)组改变更加严重.HSF1基因剔除能显著消减HSR对内毒素血症小鼠的保护作用.

  17. Ecology Sports Studies Carried Out in School Sports

    Shuyu, Xia

    In the 1970 s, people began to care for the environment and concern about the ecology thinking in sports, to study the modern sports facing the ecology problems, to explore "people, sports, environment" the harmonious development of the way. In the paper from the actual conditions of the school briefly discuss the advantages of carrying out 3.3 no complete teaching schemas and the main difficulties it faces, and then propose appropriate measures to provide a theoretical basis for the ecology sports better carried out in school sports.

  18. Structures of HSF2 Reveal Mechanisms for Differential Regulation of Human Heat Shock Factors

    Jaeger, Alex M.; Pemble, Charles W.; Sistonen, Lea; Thiele, Dennis J.

    2016-01-01

    Heat Shock Transcription Factor (HSF) family members function in stress protection and in human disease including proteopathies, neurodegeneration and cancer. The mechanisms that drive distinct post-translational modifications, co-factor recruitment and target gene activation for specific HSF paralogs are unknown. We present high-resolution crystal structures of the human HSF2 DNA-binding domain (DBD) bound to DNA, revealing an unprecedented view of HSFs that provides insights into their unique biology. The HSF2 DBD structures resolve a novel carboxyl-terminal helix that directs the coiled-coil domain to wrap around DNA, exposing paralog-specific sequences of the DBD surface, for differential post-translational modifications and co-factor interactions. We further demonstrate a direct interaction between HSF1 and HSF2 through their coiled-coil domains. Together, these features provide a new model for HSF structure as the basis for differential and combinatorial regulation to influence the transcriptional response to cellular stress. PMID:26727490

  19. Study of tourist motivation to Guangzhou urban ecological parks

    Li, Min; Wang, Fengtang

    2012-01-01

    Based on the push - pull theory, this article show the empirical studies of tourists' travel motivation to Guangzhou Ecological Park. We identified four push factors and three pull factors which are of the potential features of travel motivation, and on this basis, using analysis of variance to further test the significant differences of push and pull factors in the demographic characteristics of different groups. The conclusion has a certain reference value to the Ecological Park in Guangzhou to attract tourists.

  20. Cloning and expression of HSF1 cDNA from Hainan Eld's deer%海南坡鹿HSF1cDNA的克隆与表达

    成鹰; 林杰材; 满初日嘎; 杜丽; 王凤阳; 刘涛; 李治深; 许世英; 符运南; 林贤梅; 吴科榜

    2009-01-01

    采用RT-PCR和RACE方法扩增海南坡鹿热休克转录调节因子1(Heat shock transcription factor 1,HSF1)cDNA全长,将扩增产物与pMD20-T载体连接,重组质粒经PCR、酶切鉴定后测序并进行生物信息学分析;构建pET28a-hdHSF1表达载体,经IPTG诱导表达后,进行SDS-PAGE和Western blot分析.结果显示,海南坡鹿HSF1cDNA全长为2 036 bp,含有1个1 578 bp的开放阅读框,编码525个氨基酸.经生物信息学分析,HSF1是一个等电点为4.93的亲水性蛋白.经IPTG诱导表达后,得到一个带组氨酸标签的约62 kD的融合蛋白,用抗His单克隆抗体进行Western blot,得到一条约62 kD特异性抗体结合带,表明海南坡鹿HSF1原核表达载体成功构建并表达.

  1. Behavioral Characterization of HSF1 Knockout Male Mice%雄性HSF1基因缺陷小鼠的行为改变

    朱熊兆; 程明; 彭敏; 姚树桥

    2007-01-01

    为研究HSF1基因缺陷小鼠的行为特征,探索HSF1基因在小鼠行为表现中的作用.选取6~7个月大雄性HSF1基因缺陷小鼠39只及野生型小鼠36只进行情绪性评分、旷场实验、高架十字迷宫实验、简易迷津实验、T-CAT实验、独木桥实验和悬挂实验以观察其情绪性唤醒水平、焦虑水平、探索行为、工作记忆能力和运动能力.结果表明HSF1基因缺陷小鼠的情绪唤醒水平和焦虑水平较低、探索行为减少、T-CAT中转换率较低,提示小鼠的情绪、探索动机和工作记忆受HSF1基因的调控.

  2. 不同hsf1基因型对小鼠心肌组成型αBC表达的影响%EFFECTS OF hsf1 GENOTYPE ON THE CONSTITUTIVE EXPRESSION OF αB-CRYSTALLIN IN MICE MYOCARDIUM

    刘莉; 张红慧; 丁国宪; 程蕴琳; 晏良军; Benjamin I J

    2004-01-01

    目的:了解热休克转录因子1(heat shock transcription factor 1,HSF1)基因对小鼠心肌组成型(B晶体蛋白(αB-Crystallin,αBC)表达的影响.方法:用Western Blot和免疫组织化学方法,测定组成型αBC在HSF1基因野生型(hsf1+ / +)和HSF1基因敲除型(hsf1- / -)小鼠心肌中的表达.结果:αBC在hsf1- / - 和 hsf1+ / +小鼠心肌表达量分别为68.42%±4.16% 和100%±7.58%(心肌可溶性组分,P<0.05),20.53%±1.01%和37.55%±1.91%(心肌不可溶性组分,P<0.05);免疫组化显示αBC在hsf1- / -心肌细胞内的表达信号较hsf1+ / +明显减弱.结论:hsf1基因是介导组成型αBC基因表达重要的、但不是唯一的因子.

  3. The Study on the ecological education of community residents based on the viewpoint of ecological civilization

    田文明; 黄辉

    2015-01-01

    The essay focuses on the analysis of necessity and urgency of the mobilization to conduct a comprehensive ecological protection for community residents.The author emphasizes the significance of strengthen community residents ecological education.Based on the present situation of the ecological education of community residents,community residents' ecological education is carried out,has great significance and importance.

  4. Expression and Purification of Heat Shock Factor HSF1 in Arabidopsis thaliana%拟南芥热激因子HSF1的表达与纯化

    郭丽红; 王定康; 袁燕; 刘开庆; 张乐民

    2009-01-01

    [目的] 表达和纯化拟南芥热激因子HSF1.[方法] 以构建的能表达热激因子HSF1的大肠杆菌Escherichia coli M15(pQE32/ His6- HSF1,pREP4)为材料,用异丙基硫代-β-D-半乳糖苷(IPTG)诱导表达HSF1,再通过镍亲和层析纯化表达的HSF1,通过变性的聚丙酰胺(SDS-PAGE)电泳分析表达蛋白和纯化蛋白.[结果] 试验获得了表达的HSF1,并且进一步获得了纯化的HSF1.[结论]该研究为探讨拟南芥HSF1在基因组的结合位点提供了试验材料,为全面认识HSF1作用机理和生理功能奠定了基础.

  5. Stable isotope methods in biological and ecological studies of arthropods

    Hood-Nowotny, R.C.; Knols, B.G.J.

    2007-01-01

    This is an eclectic review and analysis of contemporary and promising stable isotope methodologies to study the biology and ecology of arthropods. It is augmented with literature from other disciplines, indicative of the potential for knowledge transfer. It is demonstrated that stable isotopes can b

  6. Investigations on biological functions of heat shock transcription factor 1 (HSF1) using a gene knock out mouse model

    2001-01-01

    HSF1 is the major heat shock transcription factor that binds heat shock element (HSE) in the promoter of heat shock proteins (HSPs) and controls rapid HSP induction in cells subjected to various stresses such as elevated temperature, chemicals, or exposure to toxins. Although at least four members of the vertebrate HSF have been cloned, details of their individual physiological roles remain relatively obscure. To clarify the exact in vivo functions of HSF1 and assess whether HSF1 exhibits redundant or unique roles, we have created homozygous Hsf1-/- mice using standard gene targeting techniques and isolated Hsf1-/- embryonic fibroblasts. Here we demonstrate that heat shock response (HSR) was not attainable in Hsf1-/- embryonic fibroblasts, and this response was required for thermotolerance and protection against heat-induced apoptosis, and that homozygous Hsf1-/- mice, which survived to adulthood according to genetic background, exhibited multiple phenotypes including: (1) placental defects that reduced embryonic viability after late midgestation (day 13.5); (2) growth retardation; (3) female infertility caused by preimplantation lethality, and (4) increased mortality (+/+ vs -/-, P<0.05) and exaggerated production of proinflammatory cytokine, TNF α (+/- vs -/-, P<0.05) after endotoxin challenge. Interestingly, although Hsf1-/- mice exhibited placental defects and embryonic death, basal HSP expression is not appreciably altered during embryonic development by the HSF1 null mutation, suggesting this factor might be involved in regulating some non-HSP genes or signaling pathways which may be important for development. Taken together, our results established direct causal effects for the HSF1 transactivator in regulating diverse physiological and pathophysiological conditions such as developnent, growth, reproduction, apoptosis and sepsis. The present work also provided a useful mammalian model for further investigating the implications of Hsf1 and its target

  7. Radiological Review Studies On Ismailia Canal Ecology

    The present work is a brief review of pr-studies carried out on Ismailia Canal, Egypt, water ecosystem. Ismailia Canal water body is a bicarbonate stream with slight seasonal variations in its water chemical constituents. The canal water pH in all the stream locations are below 8.3 with low suspended matter(SM) content (22-33 mg.l-1). The mineralogical analysis of the canal bottom sediments consist mainly of quartz, smectite and kaolinite minerals. The γ- spectroscopic identification showed traces of naturally occurring radio nuclides (238U, 232Th and 40K). The average activity level of the dry samples ranged from 12 to 89 Bq.Kg-1 for the detected natural radio nuclides. Some parameters affected the sorption behaviour of radio nuclides on suspended matters and bottom sediments; such as solution pH, SM concentration, sediment grain size, carrier concentration and competing ions were studied. The reaction rates were investigated for each radionuclide studied. The distribution of the studied radio nuclides, between the liquid phase and the sediments phase was investigated, for both flowing and static systems. For both flowing and non-flowing (static), the depth penetration of the studied radio nuclides within the bottom sediment layers were found to vary from one radionuclide to the other. The total capacities of bottom sediments and the suspended matter were found to be low. As Ismailia Canal is an important source of water for public domestic uses, irrigation animals and the aquatic species; these situations have led to state that it is not recommended to release any liquid radioactive wastes to this canal. Furthermore, periodical radiometric analysis for the canal water and its components should be carried out.

  8. Effect of democracy on health: ecological study

    Franco, Álvaro; Álvarez-Dardet, Carlos; Ruiz, Maria Teresa

    2004-01-01

    Can political regimes be singled out as a factor affecting health? Rating countries by the extent of their freedom is a useful proxy for measuring the effects of democracy on health related variables. Although the influence of democracy in preventing famines has been reported, there have been no empirical studies on the relation between the extent of freedom allowed by political regimes and the effect on a nation’s health. We explored the effect of democracy on life expectancy and...

  9. Science and ecological literacy in undergraduate field studies education

    Mapp, Kim J.

    There is an ever-increasing number of issues that face our world today; from climate change, water and food scarcity, to pollution and resource extraction. Science and ecology play fundamental roles in these problems, and yet the understanding of these fields is limited in our society (Miller, 2002; McBride, Brewer, Berkowitz, and Borrie, 2013). Across the nation students are finishing their undergraduate degrees and are expected to enter the workforce and society with the skills needed to succeed. The deficit of science and ecological literacy in these students has been recognized and a call for reform begun (D'Avanzo, 2003 and NRC, 2009). This mixed-methods study looked at how a field studies course could fill the gap of science and ecological literacy in undergraduates. Using grounded theory, five key themes were data-derived; definitions, systems thinking, human's role in the environment, impetus for change and transference. These themes where then triangulated for validity and reliability through qualitative and quantitative assessments. A sixth theme was also identified, the learning environment. Due to limited data to support this themes' development and reliability it is discussed in Chapter 5 to provide recommendations for further research. Key findings show that this field studies program influenced students' science and ecological literacy through educational theory and practice.

  10. HSF1基因表达升高与大肠癌%Induction of HSF1 expression and sporadic colorectal cancer

    方永明; 董琦; 岑辉; 唐小萍; 郑树

    2004-01-01

    目的:探讨散发性人结直肠癌发生发展中可能的信号转导通路.方法:应用8条信号通路基因芯片筛选结直肠癌组织与正常粘膜组织表达差异基因;提取35例结直肠癌患者配对的癌组织及正常粘膜组织(阴性切缘组织,距肿瘤10 cm以上)总RNA,以RT-PCR的方法对有差异的基因进行表达差异比较.结果:结直肠癌组织中hsf1、hsp27及inos的表达明显高于正常组织.经35例结直肠癌患者癌组织与正常大肠粘膜组织对比,癌组织中hsf1、hsp27、inos表达增高,其中hsf1为86%(30/35),inos为63%(22/35).结论:在结直肠癌组织中hsf1、hsp27及inos基因被激活,其中可能存在热刺激应激信号转导通路激活的通道.

  11. Characterizing HSF1 Binding and Post-Translational Modifications of hsp70 Promoter in Cultured Cortical Neurons: Implications in the Heat-Shock Response.

    Andrea V Gómez

    Full Text Available Causes of lower induction of Hsp70 in neurons during heat shock are still a matter of debate. To further inquire into the mechanisms regulating Hsp70 expression in neurons, we studied the activity of Heat Shock Factor 1 (HSF1 and histone posttranslational modifications (PTMs at the hsp70 promoter in rat cortical neurons. Heat shock induced a transient and efficient translocation of HSF1 to neuronal nuclei. However, no binding of HSF1 at the hsp70 promoter was detected while it bound to the hsp25 promoter in cortical neurons during heat shock. Histone PTMs analysis showed that the hsp70 promoter harbors lower levels of histone H3 and H4 acetylation in cortical neurons compared to PC12 cells under basal conditions. Transcriptomic profiling data analysis showed a predominant usage of cryptic transcriptional start sites at hsp70 gene in the rat cerebral cortex, compared with the whole brain. These data support a weaker activation of hsp70 canonical promoter. Heat shock increased H3Ac at the hsp70 promoter in PC12 cells, which correlated with increased Hsp70 expression while no modifications occurred at the hsp70 promoter in cortical neurons. Increased histone H3 acetylation by Trichostatin A led to hsp70 mRNA and protein induction in cortical neurons. In conclusion, we found that two independent mechanisms maintain a lower induction of Hsp70 in cortical neurons. First, HSF1 fails to bind specifically to the hsp70 promoter in cortical neurons during heat shock and, second, the hsp70 promoter is less accessible in neurons compared to non-neuronal cells due to histone deacetylases repression.

  12. Hsp90 orchestrates transcriptional regulation by Hsf1 and cell wall remodelling by MAPK signalling during thermal adaptation in a pathogenic yeast.

    Michelle D Leach

    2012-12-01

    Full Text Available Thermal adaptation is essential in all organisms. In yeasts, the heat shock response is commanded by the heat shock transcription factor Hsf1. Here we have integrated unbiased genetic screens with directed molecular dissection to demonstrate that multiple signalling cascades contribute to thermal adaptation in the pathogenic yeast Candida albicans. We show that the molecular chaperone heat shock protein 90 (Hsp90 interacts with and down-regulates Hsf1 thereby modulating short term thermal adaptation. In the longer term, thermal adaptation depends on key MAP kinase signalling pathways that are associated with cell wall remodelling: the Hog1, Mkc1 and Cek1 pathways. We demonstrate that these pathways are differentially activated and display cross talk during heat shock. As a result ambient temperature significantly affects the resistance of C. albicans cells to cell wall stresses (Calcofluor White and Congo Red, but not osmotic stress (NaCl. We also show that the inactivation of MAP kinase signalling disrupts this cross talk between thermal and cell wall adaptation. Critically, Hsp90 coordinates this cross talk. Genetic and pharmacological inhibition of Hsp90 disrupts the Hsf1-Hsp90 regulatory circuit thereby disturbing HSP gene regulation and reducing the resistance of C. albicans to proteotoxic stresses. Hsp90 depletion also affects cell wall biogenesis by impairing the activation of its client proteins Mkc1 and Hog1, as well as Cek1, which we implicate as a new Hsp90 client in this study. Therefore Hsp90 modulates the short term Hsf1-mediated activation of the classic heat shock response, coordinating this response with long term thermal adaptation via Mkc1- Hog1- and Cek1-mediated cell wall remodelling.

  13. Terrestrial ecology. Comprehensive study of the grassland biome

    Terrestrial ecology and grassland biome studies are designed to characterize the biota of the Hanford Reservation, elucidate seasonal dynamics of plant productivity, decomposition and mineral behavior patterns of important plant communities, and, to study the response of these communities to important natural environmental stresses, such as weather, wildfire and man-induced alterations of communities (influenced by grazing cattle and severe mechanical disturbance of the soil, such as affected by plowing or burial of waste materials or construction activities). A detailed account of the important findings of a 5-yr study is currently being prepared by the terrestrial ecology section staff for publication as a contribution to the International Biological Program Grassland Biome project

  14. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator.

    Kenji Ishida

    Full Text Available Schistosomiasis is a chronically debilitating disease caused by parasitic worms of the genus Schistosoma, and it is a global problem affecting over 240 million people. Little is known about the regulatory proteins and mechanisms that control schistosome host invasion, gene expression, and development. Schistosome larvae, cercariae, are transiently free-swimming organisms and infectious to man. Cercariae penetrate human host skin directly using proteases that degrade skin connective tissue. These proteases are secreted from anucleate acetabular glands that contain many proteins, including heat shock proteins. Heat shock transcription factors are strongly conserved activators that play crucial roles in the maintenance of cell homeostasis by transcriptionally regulating heat shock protein expression. In this study, we clone and characterize the schistosome Heat shock factor 1 gene (SmHSF1. We verify its ability to activate transcription using a modified yeast one-hybrid system, and we show that it can bind to the heat shock binding element (HSE consensus DNA sequence. Our quantitative RT-PCR analysis shows that SmHSF1 is expressed throughout several life-cycle stages from sporocyst to adult worm. Interestingly, using immunohistochemistry, a polyclonal antibody raised against an Hsf1-peptide demonstrates a novel localization for this conserved, stress-modulating activator. Our analysis suggests that schistosome Heat shock factor 1 may be localized to the acetabular glands of infective cercariae.

  15. Induction of HSF1 expression is associated with sporadic colorectal cancer

    Hui Cen; Shu Zheng; Yong-Ming Fang; Xiao-Ping Tang; Qi Dong

    2004-01-01

    AIM: To explore the activation of signal transduction pathways related with the carcinogenesis of sporadic colon cancers.METHODS: A gene array monitoring the activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissues. The differentially expressed genes were further analyzed by RT-PCR, using RNA derived from colorectal cancer and normal colon tissue of 35 patients.RESULTS: The expression of HSF1, HSF27, HSP90 and iNOS was increased in colon cancer tissues compared to normal colon tissue using PathwayFinder GEArray. The RT-PCR results showed that the expression of HSF1 was increased in 86% (30/35) patients and the expression of iNOS was increased in 63% (22/35) patients.CONCLUSION: The induction of HSF1 gene expression is associated with sporadic colon cancer. HSF1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer.

  16. Hsf1 Is Required for the Nuclear Translocation of p53 Tumor Suppressor

    Qiang Li

    2008-10-01

    Full Text Available Although the p53 tumor suppressor is most frequently inactivated by genetic mutations, exclusion from the nucleus is also seen in human tumors. We have begun to examine p53 nuclear importation by isolating a series of mutant cells in which the temperature-sensitive murine p53Val135 mutant is sequestered in the cytoplasm. We previously showed that that three of them (ALTR12, ALTR19, and ALTR25 constituted a single complementation group. Here, we found that ALTR12 cells are more sensitive to heat stress than either ALTR19 or ALTR25 and that there was a complete lack of induction of Hsp70 in response to heat shock. Western blot analysis showed no expression of the Hsf1 transcription factor, and neither heat shock nor azetidine could induce p53 nuclear localization in ALTR12 cells but did in parental A1–5 cells. Suppression of Hsf1 in A1–5 cells with quercetin or an Hsf1 siRNA reduced p53 nuclear importation and inhibited p53-mediated activation of a p21 reporter. Most convincingly, p53 nuclear importation could be restored in ALTR12 cells by introducing an exogenous Hsf1 gene. Collectively, our result suggests that Hsf1 is required for p53 nuclear importation and activation and implies that heat shock factors play a role in the regulation of p53.

  17. Temporal and Spatial Melanoma Trends in Austria: An Ecological Study

    Daniela Haluza; Stana Simic; Hanns Moshammer

    2014-01-01

    Annual solar ultraviolet radiation (UVR) is mostly determined by latitude and altitude. Over the last decades, increasing UVR ground levels have been observed. Exposure to UVR is associated with a life-time risk to develop melanoma, a malign skin cancer. Thus, we hypothesized that melanoma incidence in Austria is associated with altitude of place of living and time of diagnosis. We investigated this hypothesis in an ecological study by district and year for Austrian melanoma incidence ...

  18. 禁食通过 HSF1保护肝脏缺血再灌注损伤%Fasting protects mice against hepatic ischemia/reperfusion injury through HSF1

    崔小兰; 张常明; 李大伟; 张江; 夏强

    2015-01-01

    目的:探讨短期禁食对肝脏缺血再灌注损伤的影响及和 HSF1的关系。方法小鼠随机分为两大组8小组,第一大组为假手术组,再进一步分为4个小组:A 组 HSF1Alb-小鼠正常饮食,B 组 HSF1Alb-小鼠禁食16 h,C 组HSF1Alb+小鼠正常饮食,D 组 HSF1Alb+小鼠禁食16 h。对照组只进行开关腹和游离左外叶不进行阻断和开放左外叶血流;第二大组为缺血再灌注组,进一步分为4个小组:E 组 HSF1Alb-小鼠正常饮食,F 组 HSF1Alb-小鼠禁食16 h,G 组HSF1Alb+小鼠正常饮食,H 组 HSF1Alb+小鼠禁食16 h。缺血再灌注组进行小鼠左外叶30%的缺血60 min 再灌注6 h实验。检测各组小鼠血清 ALT、AST。通过比较各组血清转氨酶的水平以及评价肝组织病理损伤的程度,来判断短期禁食及 HSF1对小鼠缺血再灌注损伤的影响。结果假手术组禁食和不禁食对血清转氨酶没有明显差别,缺血再灌注组中, HSF1Alb-小鼠禁食组的转氨酶明显低于正常饮食组,但在 HSF1Alb+小鼠看不到这一明显的保护作用;HSF1Alb-小鼠禁食组的肝组织损伤明显减轻。结论禁食能明显减轻肝脏缺血再灌注损伤,禁食的肝脏缺血再灌注损伤的保护作用在HSF1-/-小鼠被消除。%Objective To investigate the effect of short-term fasting on hepatic ischemia/reperfusion (IR)injury and its relationship with heat shock factor 1 (HSF1 ).Methods A hepatic ischemia/reperfusion model was built.The left lateral liver was subjected to 60 minutes of ischemia followed by 6 hours of reperfusion,then the left liver and blood was collected.Male HSF1Alb-and HSF1Alb+ mice were randomly divided into 2 groups:control group (with false operation);IR group,which were subjected to ischemia for 60min and reperfusion for 6h.Furthermore,both groups were divided into 4 subgroups according to the fasting and HSF1Alb.Levels of alanine aminotransferase and aspartate

  19. Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis.

    Solís, Eric J; Pandey, Jai P; Zheng, Xu; Jin, Dexter X; Gupta, Piyush B; Airoldi, Edoardo M; Pincus, David; Denic, Vladimir

    2016-07-01

    Despite its eponymous association with the heat shock response, yeast heat shock factor 1 (Hsf1) is essential even at low temperatures. Here we show that engineered nuclear export of Hsf1 results in cytotoxicity associated with massive protein aggregation. Genome-wide analysis revealed that Hsf1 nuclear export immediately decreased basal transcription and mRNA expression of 18 genes, which predominately encode chaperones. Strikingly, rescuing basal expression of Hsp70 and Hsp90 chaperones enabled robust cell growth in the complete absence of Hsf1. With the exception of chaperone gene induction, the vast majority of the heat shock response was Hsf1 independent. By comparative analysis of mammalian cell lines, we found that only heat shock-induced but not basal expression of chaperones is dependent on the mammalian Hsf1 homolog (HSF1). Our work reveals that yeast chaperone gene expression is an essential housekeeping mechanism and provides a roadmap for defining the function of HSF1 as a driver of oncogenesis. PMID:27320198

  20. A Direct Regulatory Interaction between Chaperonin TRiC and Stress-Responsive Transcription Factor HSF1

    Daniel W. Neef

    2014-11-01

    Full Text Available Heat shock transcription factor 1 (HSF1 is an evolutionarily conserved transcription factor that protects cells from protein-misfolding-induced stress and apoptosis. The mechanisms by which cytosolic protein misfolding leads to HSF1 activation have not been elucidated. Here, we demonstrate that HSF1 is directly regulated by TRiC/CCT, a central ATP-dependent chaperonin complex that folds cytosolic proteins. A small-molecule activator of HSF1, HSF1A, protects cells from stress-induced apoptosis, binds TRiC subunits in vivo and in vitro, and inhibits TRiC activity without perturbation of ATP hydrolysis. Genetic inactivation or depletion of the TRiC complex results in human HSF1 activation, and HSF1A inhibits the direct interaction between purified TRiC and HSF1 in vitro. These results demonstrate a direct regulatory interaction between the cytosolic chaperone machine and a critical transcription factor that protects cells from proteotoxicity, providing a mechanistic basis for signaling perturbations in protein folding to a stress-protective transcription factor.

  1. Ecological interaction and phylogeny, studying functionality on composed networks

    Cruz, Claudia P. T.; Fonseca, Carlos Roberto; Corso, Gilberto

    2012-02-01

    We study a class of composed networks that are formed by two tree networks, TP and TA, whose end points touch each other through a bipartite network BPA. We explore this network using a functional approach. We are interested in how much the topology, or the structure, of TX (X=A or P) determines the links of BPA. This composed structure is a useful model in evolutionary biology, where TP and TA are the phylogenetic trees of plants and animals that interact in an ecological community. We make use of ecological networks of dispersion of fruits, which are formed by frugivorous animals and plants with fruits; the animals, usually birds, eat fruits and disperse their seeds. We analyse how the phylogeny of TX determines or is correlated with BPA using a Monte Carlo approach. We use the phylogenetic distance among elements that interact with a given species to construct an index κ that quantifies the influence of TX over BPA. The algorithm is based on the assumption that interaction matrices that follows a phylogeny of TX have a total phylogenetic distance smaller than the average distance of an ensemble of Monte Carlo realisations. We find that the effect of phylogeny of animal species is more pronounced in the ecological matrix than plant phylogeny.

  2. Study of ecological situation for the West Kazakhstan south districts

    In this paper the results of comprehensive research for ecological status of Karabota, Kaztal, Dzhangaly, Dzhanibek and Akzhaik districts of the West Kazakhstan districts adjoining to 'Kapustin Yar' and Azgir' test sites are presented. In the indicated districts the following examinations were conducted: analysis of soils samples; bottom sediments, flora and fauna samples for determination of radionuclide and heavy metals content in its; determination of absorption dose with population by the EPR dosimetry method by human teeth enamel; study of α-radiation accumulation in a diverse trees' annual rings

  3. Study on RS- and GIS-based ecological capital assessment in arid areas

    ZHOU Kefa; CHEN Xi; ZHOU Huarong; ZHANG Qing; ZUO Qiting; ZHANG Haibo; YAN Jinfeng; CHEN Chuan

    2006-01-01

    Ecological capital assessment is currently one of the important research contents in the interdisciplinary field of ecology, resources economics, environmental economics and ecological economics. To scientifically assess ecological capital redounds to decision-makers to consider the ecological cost in economic development, and is also necessary for sustainable economic development in arid areas. Based on the theory of landscape ecology,in this paper the per unit area ecological capital values in arid areas are researched by using the Landsat TM data, CBERS satellite data, meteorological data, MODIS satellite data and other ecological data,and the RS- and GIS-based models of assessing ecological capital values in arid areas are developed.Moreover, based on the field-measured data, a case study on ecological capital assessment in the Manas River basin, Xinjiang in 2003 is carried out. The basin is divided into 4 ecological capital areas so as to quantitatively calculate the ecological capital values of the ecosystems, analyze the spatial distribution of ecological capital, and chart the maps of spatial distribution of ecological capital. The results show that the total ecological capital value of the ecosystems in the Manas River basin in 2003 was 1.49454×1011yuan RMB. In spatial distribution, the ecological capital decreases from the alpine zones to the plains and from the oases to the deserts, which accords with the distribution of vegetation zonality in this arid area.The assessed results can objectively reflect the ecological capital and its spatial distribution in the arid basin, and can also provide reference for roundly carrying out the assessment of ecological capital in arid areas.

  4. Isolation of a cDNA for HSF 2: Evidence for two heat shock factor genes in humans

    Schuetz, T.J.; Gallo, G.J.; Sheldon, L.; Kingston, R.E. (Massachusetts General Hospital, Boston (United States) Harvard Medical School, Boston, MA (United States)); Tempst, P. (Harvard Medical School, Boston, MA (United States))

    1991-08-15

    The heat shock response is transcriptionally regulated by an evolutionarily conserved protein termed heat shock factor (HSF). The authors report the purification to homogeneity and the partial peptide sequence of HSF from HeLa cells. The peptide sequence was used to isolate a human cDNA with a predicted open reading frame that has homology to the DNA binding domains of both Saccharomyces cerevisiae and Drosophila HSFs. The cDNA directs the synthesis of a protein that binds to the heat shock element with specificity identical to HeLa HSF and stimulates transcription from a heat shock promoter. The expressed protein cross-reacts with anti-HSF antibodies. Surprisingly, however, this cDNA does not encode all of the peptides obtained from purified HeLa HSF. These peptides are encoded by a distinct human cDNA. HSF1. It therefore appears that there is a human heat shock factor gene family and that at least two separate but related HSF proteins regulate the stress response in humans.

  5. 大鼠再生肝中hsbp1、hsf1、hsf2、hsp70表达水平改变的分析%ANALYSIS OF CHANGES ABOUT hsbp1, hsf1, hsf2 AND hsp70'S EXPRESSION LEVELS IN RAT'S REGENERATING LIVER

    苏丽娟; 常翠芳; 韩鸿鹏; 马辉; 徐存拴

    2006-01-01

    在克隆了大鼠热休克因子结合蛋白1基因(hsbp1)全长cDNA基础上,进一步分析它在肝再生中作用.用SD纯系大鼠为材料,按Higgens等方法建立大鼠部分肝切除(PH)模型;用原位杂交等方法分析hsbp1在肝再生中表达变化;用基因表达谱芯片分析hsbp1、hsf1、hsf2和hsp70在肝再生中表达变化.原位杂交和基因表达谱芯片分析表明,PH后6h和66-144h,hsp1表达发生了有意义上调;8-16h,hsf1表达发生了有意义上调;2-16h,hsf2表达发生了有意义上调;0.5-24h,hsp70表达发生了有意义上调.假手术(只打开腹腔和翻动肝叶,但不进行部分肝切除)后0.5-2h,hsbp1表达发生了有意义下调;8-16h,hsf1表达发生了有意义上调;0-144h,hsf2未发生有意义表达变化;0.5-30h,hsp70表达发生了有意义上调.根据实验结果推测,PH后hsbp1表达上调可增加细胞内HSBP1量,促进生长、发育、分化相关基因表达和再生肝的组织结构功能重建;(假)手术后hsbp1表达下调可减少细胞内HSBP1量,有利于HSF1上调hsp70表达,提高机体和肝脏抗损伤能力.

  6. The translational study of apathy – an ecological approach

    Flurin eCathomas

    2015-09-01

    Full Text Available Apathy, a quantitative reduction in goal-directed behavior, is a prevalent symptom dimension with a negative impact on functional outcome in various neuropsychiatric disorders including schizophrenia and depression. The aim of this review is to show that interview-based assessment of apathy in humans and observation of spontaneous rodent behavior in an ecological setting can serve as an important complementary approach to already existing task-based assessment, to study and understand the neurobiological bases of apathy. We first discuss the paucity of current translational approaches regarding animal equivalents of psychopathological assessment of apathy. We then present the existing evaluation scales for the assessment of apathy in humans and propose five sub-domains of apathy, namely self-care, social interaction, exploration, work/education and recreation. Each of the items in apathy evaluation scales can be assigned to one of these sub-domains. We then show that corresponding, well-validated behavioral readouts exist for rodents and that, indeed, three of the five human apathy sub-domains have a rodent equivalent. In conclusion, the translational ecological study of apathy in humans and mice is possible and will constitute an important approach to increase the understanding of the neurobiological bases of apathy and the development of novel treatments.

  7. Unmanned Aircraft Systems complement biologging in spatial ecology studies.

    Mulero-Pázmány, Margarita; Barasona, Jose Ángel; Acevedo, Pelayo; Vicente, Joaquín; Negro, Juan José

    2015-11-01

    The knowledge about the spatial ecology and distribution of organisms is important for both basic and applied science. Biologging is one of the most popular methods for obtaining information about spatial distribution of animals, but requires capturing the animals and is often limited by costs and data retrieval. Unmanned Aircraft Systems (UAS) have proven their efficacy for wildlife surveillance and habitat monitoring, but their potential contribution to the prediction of animal distribution patterns and abundance has not been thoroughly evaluated. In this study, we assess the usefulness of UAS overflights to (1) get data to model the distribution of free-ranging cattle for a comparison with results obtained from biologged (GPS-GSM collared) cattle and (2) predict species densities for a comparison with actual density in a protected area. UAS and biologging derived data models provided similar distribution patterns. Predictions from the UAS model overestimated cattle densities, which may be associated with higher aggregated distributions of this species. Overall, while the particular researcher interests and species characteristics will influence the method of choice for each study, we demonstrate here that UAS constitute a noninvasive methodology able to provide accurate spatial data useful for ecological research, wildlife management and rangeland planning. PMID:26640661

  8. Ecological Compensation Mechanism of Agricultural Water Resources: An Empirical Study in Zhejiang, China

    Qichang Hu

    2015-01-01

    Ecological compensation is a new area in the field of resources environment. In this study, we set an empirical analysis model about ecological compensation mechanism and analyze influential factors based on agricultural perspective. The result shows that: first, loss of development opportunities is the most important factor that will affect ecological compensation, the regression coefficient is 0.071; sec, environmental protection cost is the most significant variable in ecological compensat...

  9. Ecological speciation in the tropics: insights from comparative genetic studies in Amazonia

    Beheregaray, Luciano B.; Cooke, Georgina M.; Chao, Ning L.; Landguth, Erin L.

    2015-01-01

    Evolution creates and sustains biodiversity via adaptive changes in ecologically relevant traits. Ecologically mediated selection contributes to genetic divergence both in the presence or absence of geographic isolation between populations, and is considered an important driver of speciation. Indeed, the genetics of ecological speciation is becoming increasingly studied across a variety of taxa and environments. In this paper we review the literature of ecological speciation in the tropics. W...

  10. A DNA sequence directed mutual transcription regulation of HSF1 and NFIX involves novel heat sensitive protein interactions.

    Umashankar Singh

    Full Text Available BACKGROUND: Though the Nuclear factor 1 family member NFIX has been strongly implicated in PDGFB-induced glioblastoma, its molecular mechanisms of action remain unknown. HSF1, a heat shock-related transcription factor is also a powerful modifier of carcinogenesis by several factors, including PDGFB. How HSF1 transcription is controlled has remained largely elusive. METHODOLOGY/PRINCIPAL FINDINGS: By combining microarray expression profiling and a yeast-two-hybrid screen, we identified that NFIX and its interactions with CGGBP1 and HMGN1 regulate expression of HSF1. We found that CGGBP1 organizes a bifunctional transcriptional complex at small CGG repeats in the HSF1 promoter. Under chronic heat shock, NFIX uses CGGBP1 and HMGN1 to get recruited to this promoter and in turn affects their binding to DNA. Results show that the interactions of NFIX with CGGBP1 and HMGN1 in the soluble fraction are heat shock sensitive due to preferential localization of CGGBP1 to heterochromatin after heat shock. HSF1 in turn was found to bind to the NFIX promoter and repress its expression in a heat shock sensitive manner. CONCLUSIONS/SIGNIFICANCE: NFIX and HSF1 exert a mutual transcriptional repressive effect on each other which requires CGG repeat in HSF1 promoter and HSF1 binding site in NFIX promoter. We unravel a unique mechanism of heat shock sensitive DNA sequence-directed reciprocal transcriptional regulation between NFIX and HSF1. Our findings provide new insights into mechanisms of transcription regulation under stress.

  11. New methodology for studying the structural ecology of occlusal caries

    Dige, Irene; Grønkjær, Lene; Nyvad, Bente

    Microbiological studies of occlusal dental biofilms have hitherto been hampered by inaccessibility to the sampling site and demolition of the original biofilm architecture. The aim of the present study was to explore the spatial distribution of bacterial taxa in vivo at various stages of occusal...... caries, applying a new methodology involving preparation of embedded hard dental tissue slices for fluorescence in situ hybridization (FISH) and confocal microscopy. 11 extracted teeth were included in the study and classified according to their occlusal caries status (active/inactive/sound; cavitated...... related to histological features of lesion penetration. The sites showed distinct differences in the bacterial composition and fluorescence intensity between different ecological niches in occlusal caries. Biofilm observed along the entrance of fissures showed an inner layer of microorganisms organized in...

  12. Study on ecological structures of coastal lakes in Antarctic continent

    2000-01-01

    Coastal region on the Antarctic continent, where it is under the influences both of ocean and ice sheet, as well as frequent human activities, could be considered as a fragile zone in Antarctic ecological environment. There are many lakes in coastal region, showing much differences from each other in physical-chemical features because of individual evolutionary history in their geographical environments, and suffering from different outside factors, such as climate changes and precipitation. Thus, it results in respective biological distribution and ecological structure in lakes. The present paper reports the results from the studies of chemical components, species distributions and community structures, which mainly consisted of planktons in lakes in the Vestfold Hills (68°38'S, 78°06'E), and the Larsemann Hills (69°30'S, 76°20'E), East Antarctica. It also treats the biological diversities and nutrient relationships of these different types of lakes. So as to provide more scientific basis for monitoring of climate changes and environmental protection in Antarctica.

  13. E2F, HSF2, and miR-26 in thyroid carcinoma: bioinformatic analysis of RNA-sequencing data.

    Lu, J C; Zhang, Y P

    2016-01-01

    In this study, we examined the molecular mechanism of thyroid carcinoma (THCA) using bioinformatics. RNA-sequencing data of THCA (N = 498) and normal thyroid tissue (N = 59) were downloaded from The Cancer Genome Atlas. Next, gene expression levels were calculated using the TCC package and differentially expressed genes (DEGs) were identified using the edgeR package. A co-expression network was constructed using the EBcoexpress package and visualized by Cytoscape, and functional and pathway enrichment of DEGs in the co-expression network was analyzed with DAVID and KOBAS 2.0. Moreover, modules in the co-expression network were identified and annotated using MCODE and BiNGO plugins. Small-molecule drugs were analyzed using the cMAP database, and miRNAs and transcription factors regulating DEGs were identified by WebGestalt. A total of 254 up-regulated and 59 down-regulated DEGs were identified between THCA samples and controls. DEGs enriched in biological process terms were related to cell adhesion, death, and growth and negatively correlated with various small-molecule drugs. The co-expression network of the DEGs consisted of hub genes (ITGA3, TIMP1, KRT19, and SERPINA1) and one module (JUN, FOSB, and EGR1). Furthermore, 5 miRNAs and 5 transcription factors were identified, including E2F, HSF2, and miR-26. miR-26 may participate in THCA by targeting CITED1 and PLA2R1; E2F may participate in THCA by regulating ITGA3, TIMP1, KRT19, EGR1, and JUN; HSF2 may be involved in THCA development by regulating SERPINA1 and FOSB; and small-molecule drugs may have anti-THCA effects. Our results provide novel directions for mechanistic studies and drug design of THCA. PMID:26985959

  14. Packaging and distributing ecological data from multisite studies

    Olson, R.J.; Voorhees, L.D.; Field, J.M.; Gentry, M.J.

    1996-10-01

    Studies of global change and other regional issues depend on ecological data collected at multiple study areas or sites. An information system model is proposed for compiling diverse data from dispersed sources so that the data are consistent, complete, and readily available. The model includes investigators who collect and analyze field measurements, science teams that synthesize data, a project information system that collates data, a data archive center that distributes data to secondary users, and a master data directory that provides broader searching opportunities. Special attention to format consistency is required, such as units of measure, spatial coordinates, dates, and notation for missing values. Often data may need to be enhanced by estimating missing values, aggregating to common temporal units, or adding other related data such as climatic and soils data. Full documentation, an efficient data distribution mechanism, and an equitable way to acknowledge the original source of data are also required.

  15. Ecological and sociological considerations of wind energy: A multidisciplinary study

    Bicknell, Lucas John

    Wind energy is quickly becoming a critical technology for providing Americans with renewable energy, and rapid construction of wind facilities may have impacts on both wildlife and human communities. Understanding both the social and ecological issues related to wind energy development could provide a framework for effectively meeting human energy needs while conserving species biodiversity. In this research I looked at two aspects of wind energy development: public attitudes toward wind energy development and wind facility impacts on local bat populations. These papers present aspects of wind energy development that have been the subject of increasing study. This preliminary research is intended to demonstrate the responsibility we have to making well-informed decisions as we continue to expand wind energy development. Additionally, I hope to generate interest in interdisciplinary study as a means to broaden the scope of research by making use of the diverse tools available within different disciplines.

  16. Study on Ecological Compensation System and Land Desertification Control

    Lu, Zhixiang; Gao, Bingtao

    2009-01-01

    The problems of desertification control in China are analyzed from the perspective of ecological compensation. Firstly, there is no guarantee of ecological compensation system in desertification control. On the basis of knowing land desertification connotation, the western region is taken as an example to analyze the serious consequence brought by land desertification, besides, the connotation and importance of ecological compensation are summarized, as well as the views related to resolve th...

  17. Ecological tax reform - an optimal solution?. Critical remarks on the DIW study ''Economic effects of an ecological tax reform''

    Through the latest expertise of the German institute for economic research (DIW) regarding an ecological tax reform, the discussion about a tax system considering the shortage of the ressource environment and a deficiency of demand regarding the ressource work is newly provoked. The focus of this article is a critical dealing with the methodical procedure of the German institute for economic reserarch when analyzing the national economic effect of a concretely formulated ecological tax law scenario. When assessing the overall economic consequences of a tax reform, it is recommended to use an analysis instrument, which is farly more consistent compared to the instruments by DIW, and which is more problem adequate through the increased resort to financial knowledge. Based on the obvious weakness of the DIW study, a more extensive comprehension for an ecological tax reform is pleaded for, standing out for an application of taxes based on division of labour and oriented to the objective. (orig./UA)

  18. Inequality in maternal mortality in Iran: An ecologic study

    Parvin Tajik

    2012-01-01

    Full Text Available Background : Maternal mortality (MM is an avoidable death and there is national, international and political commitment to reduce it. The objective of this study is to examine the relation of MM to socioeconomic factors and its inequality in Iran′s provinces at an ecologic level. Methods : The overall MM from each province was considered for 3 years from 2004 to 2006. The five independent variables whose relations were studied included the literacy rate among men and women in each province, mean annual household income per capita, Gini coefficients in each province, and Human Development Index (HDI. The correlation of Maternal Mortality Ratio (MMR to the above five variables was evaluated through Pearson′s correlation coefficient (simple and weighted for each province′s population and linear regression-by considering MMR as the dependent variable and the Gini coefficient, HDI, and difference in literacy rate among men and women as the independent variables. Results: The mean MMR in the years 2004-2006 was 24.7 in 100,000 live births. The correlation coefficients between MMR and literacy rate among women, literacy rate among men, the mean annual household income per capita, Gini coefficient and HDI were 0.82, 0.90, −0.61, 0.52 and −0.77, respectively. Based on multivariate regression, MMR was significantly associated with HDI (standardized B=−0.93 and difference in literacy rate among men and women (standardized B=−0.47. However, MMR was not significantly associated with the Gini coefficient. Conclusion: This study shows the association between socioeconomic variables and their inequalities with MMR in Iran′s provinces at an ecologic level. In addition to the other direct interventions performed to reduce MM, it seems essential to especially focus on more distal factors influencing MMR.

  19. HSF1 Induced High Expression of KLF4 Gene in Heat-shock Response%HSF1在热休克反应中对KLF4基因表达的影响

    刘瑛; 张华莉; 袁灿; 王秋鹏; 刘梅冬; 涂自智; 肖献忠

    2004-01-01

    [目的]观察热休克因子1(HSF1)在热休克反应中对Kruppel 样因子4(KLF4)基因表达的影响;采用生物信息学方法初步探讨KLF4在热休克反应中调控的下游基因.[方法]采用HSF1基因敲除小鼠热休克模型,抽提HSF1基因敲除小鼠(HSF1-/-)和野生型小鼠(HSF1+/+)心肌及肺组织的总RNA进行RT-PCR和Northern blot实验,观察KLF4 mRNA表达的情况.用热休克处理和HSF1过表达的小鼠RAW264.7巨噬细胞,抽提总RNA进行RT-PCR实验,观察KLF4 mRNA表达的情况;用TESS分析启动子含有KLF4结合位点的下游基因.[结果]热休克处理后,HSF1+/+小鼠组织中KLF4 mRNA的水平明显增加,HSF1-/-小鼠组织中KLF4 mRNA水平的增加明显低于HSF1+/+小鼠.小鼠RAW264.7巨噬细胞受热刺激后,KLF4 mRNA的水平明显增加;在HSF1过表达细胞中KLF4的表达也明显增高.经TESS软件分析发现6个启动子区含有KLF4结合位点的下游基因.[结论]HSF1诱导KLF4基因在热休克反应中呈现高表达.

  20. Dynamic Changes of Land Ecological Carrying Capacity Based on the Ecological Footprint——By the Case Study of Chengdu City

    Wenfu PENG; Xiaolan ZHONG; Qiuyang LI; Qin LI

    2012-01-01

    Chengdu City is in the period of rapid urbanization and industrialization, and the disturbance derived from human activities on environment is increasing remarkablely in recent 20 years. The pressure on environment, economy and population is also increasing and land use in Chengdu has changed enormously. As struc- ture and function of land ecological system change obviously, sustainable development of land productivity has been an important goal and strategic task from now on, and it is necessary to systematically research land ecological carrying capacity based on ecological footprint. The ecological footprint of Chengdu City in the past ten years was calculated and analyzed from the spatial and temporal aspects according to statistical data from 1998 to 2008, as per ecological footprint method, ecological carrying capacity and the GIS spatial analysis method, and regression analysis method. The ecological footprint and ecological carrying capacity values from 2009 to 2019 in Chengdu City were predicted through calculation results in the past ten years. The results show that the ecological footprint and ecological deficit of land use from 1998 to 2008 increased in Chengdu City. The ecological deficit of land use within the city center was in high levels in the past ten years, and the ecological footprint kept raising, especially in areas, such as Shuangliu, Chongzhou, Qingyang among 9 city areas, 4 counties and 6 districts in Chengdu City. There is fanlike distribution of ecological deficit of land use. Analysis shows that the social and natural ecological system is uneven distribution, which is not in sustainable de- velopment situation. The results of the study show that the economic, social and natural ecological system in Chengdu City is not sustainable, and the ecological foot- print is uneven distribution. The analysis of the dynamic change of land ecological carrying capacity in Chengdu City is very important for city government in the pro- cess of the

  1. Role of HSF1 in spatial learning task%HSF1基因在空间学习记忆能力中的作用研究

    彭敏; 姚树桥; 朱熊兆

    2010-01-01

    目的 探讨热休克因子1(heat shock factor 1,HSF1)在小鼠Morris水迷宫空间学习任务中的作用.方法 HSF1+/+小鼠12只,HSF1-/-小鼠12只,进入空间学习任务实验.小鼠在Morris水迷宫中训练7d后,处死,剥离前额叶,液氮冻存,Western blots检测磷酸化的细胞外调节蛋白激酶(phospho-Extracellular regulated protein kinases, P-Erk1/2)表达水平.结果 HSF1-/-小鼠在Morris水迷宫定向航行实验中,第1天至第7天逃避潜伏期均明显长于HSF1+/+小鼠(P<0.05).Western blots显示:HSF1-/-小鼠在Morris水迷宫学习任务中前额叶内P-Erk1/2水平明显低于HSF1+/+小鼠(P<0.05)相应的脑区.结论 HSF1在Morris水迷宫学习任务中能够维持前额叶Erk1/2激活,因而在Morris水迷宫空间学习任务中起着重要的作用.

  2. Expression of HSF1 and XAF1 in Gastro-intestinal Cancer%HSF1与XAF1基因在胃肠肿瘤中表达的研究

    李霆; 陈村龙; 王继德; 崔生达; 崔丹瑜; 郭文

    2007-01-01

    目的 已知X连锁凋亡抑制蛋白(XIAP)相关因子1(XAF1)在胃肠癌细胞中低表达,本研究的目的 在于探讨XAF1与热休克转录因子1(HSF1)在胃肠癌中的表达情况及其相互关系.方法 应用免疫印迹分析法检测胃癌、大肠癌组织以及胃肠道肿瘤细胞株中的XAF1及HSF1蛋白表达;用含有HSF1的真核表达载体转染胃肠道肿瘤细胞株或用RNA干扰的方法上调或下调HSF1表达,同步检测对XAF1表达的影响;用应激原刺激诱导HSFl表达.观察对XAF1表达的作用.结果 在胃肠癌组织中HSF1的表达高于正常组织;在胃肠癌细胞株中XAF1与HSF1的表达呈负相关,应激刺激上调HSF1的同时下调XAF1表达.结论 胃肠道肿瘤细胞高表达HSF1,其结果是抑制XAF1表达,这种机制应该是XAF1在(胃肠道)肿瘤细胞中低表达并导致肿瘤细胞凋亡缺失的原因之一.

  3. Proteasome activity or expression is not altered by activation of the heat shock transcription factor Hsf1 in cultured fibroblasts or myoblasts.

    Taylor, David M; Kabashi, Edor; Agar, Jeffrey N; Minotti, Sandra; Durham, Heather D

    2005-01-01

    Heat shock proteins (Hsps) with chaperoning function work together with the ubiquitin-proteasome pathway to prevent the accumulation of misfolded, potentially toxic proteins, as well as to control catabolism of the bulk of cytoplasmic, cellular protein. There is evidence for the involvement of both systems in neurodegenerative disease, and a therapeutic target is the heat shock transcription factor, Hsf1, which mediates upregulation of Hsps in response to cellular stress. The mechanisms regulating expression of proteasomal proteins in mammalian cells are less well defined. To assess any direct effect of Hsf1 on expression of proteasomal subunits and activity in mammalian cells, a plasmid encoding a constitutively active form of Hsf1 (Hsf1act) was expressed in mouse embryonic fibroblasts lacking Hsf1 and in cultured human myoblasts. Plasmid encoding an inactivatible form of Hsf1 (Hsf1inact) served as control. In cultures transfected with plasmid hsf1act, robust expression of the major stress-inducible Hsp, Hsp70, occurred but not in cultures transfected with hsf1inact. No significant changes in the level of expression of representative proteasomal proteins (structural [20Salpha], a nonpeptidase beta subunit [20Sbeta3], or 2 regulatory subunits [19S subunit 6b, 11 Salpha]) or in chymotrypsin-, trypsin-, and caspaselike activities of the proteasome were measured. Thus, stress-induced or pharmacological activation of Hsf1 in mammalian cells would upregulate Hsps but not directly affect expression or activity of proteasomes. PMID:16184768

  4. The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response.

    Rachel Raynes

    Full Text Available The heat shock response, the cellular response to protein damaging stress, is critical in maintaining proteostasis. The heat shock response is regulated by the transcription factor HSF1, which is activated upon heat shock and other stresses to induce the expression of molecular chaperones. SIRT1 has previously been shown to activate HSF1 by deacetylating it, leading to increased DNA binding ability. We have investigated how the heat shock response may be controlled by factors influencing SIRT1 activity. We found that heat shock results in an increase in the cellular NAD(+/NADH ratio and an increase in recruitment of SIRT1 to the hsp70 promoter. Furthermore, we found that the SIRT1 modulators AROS and DBC1 have an impact on hsp70 transcription, HSF1 acetylation status, and HSF1 recruitment to the hsp70 promoter. Therefore, AROS and DBC1 are now two new targets available for therapeutic regulation of the heat shock response.

  5. A study on the measurement for forest ecological benefit

    张杰; 李绪尧; 姜秋来; 李长胜; 刘鹏; 董丹峰; 林丽莎; 徐文婷

    2000-01-01

    The indexes of dependent variables of the measurement on the forest ecological benefits were defined according to the analysis of the multiple ecological benefits of forest. This indexes system includes water-reserving, soil and water conservation, wind and sand suppression, microclimate improvement, carbon dioxide assimilation, atmosphere purification, flood and drought mitigation, tourism resource and wild creature protection benefits. The main factors from the numerous factors that affect dependent variables were chosen as independent variables. At last, a multivariate linear model was established for measurement of forest ecological benefit. With this multivariate linear model the forest ecological benefit of China was calculated. The forest ecological benefit of China is 723816 million yuan per year, which equals to 23.07% of the gross domestic product of China.

  6. Hydrodynamic and Ecological Assessment of Nearshore Restoration: A Modeling Study

    Along the Pacific Northwest coast, much of the estuarine habitat has been diked over the last century for agricultural land use, residential and commercial development, and transportation corridors. As a result, many of the ecological processes and functions have been disrupted. To protect coastal habitats that are vital to aquatic species, many restoration projects are currently underway to restore the estuarine and coastal ecosystems through dike breaches, setbacks, and removals. Information on physical processes and hydrodynamic conditions are critical for the assessment of the success of restoration actions. Restoration of a 160- acre property at the mouth of the Stillaguamish River in Puget Sound has been proposed. The goal is to restore native tidal habitats and estuary-scale ecological processes by removing the dike. In this study, a three-dimensional hydrodynamic model was developed for the Stillaguamish River estuary to simulate estuarine processes. The model was calibrated to observed tide, current, and salinity data for existing conditions and applied to simulate the hydrodynamic responses to two restoration alternatives. Responses were evaluated at the scale of the restoration footprint. Model data was combined with biophysical data to predict habitat responses at the site. Results showed that the proposed dike removal would result in desired tidal flushing and conditions that would support four habitat types on the restoration footprint. At the estuary scale, restoration would substantially increase the proportion of area flushed with freshwater (< 5 ppt) at flood tide. Potential implications of predicted changes in salinity and flow dynamics are discussed relative to the distribution of tidal marsh habitat.

  7. Identification of MicroRNA SNPs of HSF1 Gene and Their Association with Heat Tolerance in Chinese Holstein%中国荷斯坦牛HSF1基因microRNA SNPs与耐热性能的相关性研究

    李秋玲; 鞠志花; 贾祥捷; 黄金明; 李建斌; 李荣岭; 李芳; 王长法; 仲跻峰

    2011-01-01

    [Objective] Heat shock transcription factorl (HSF1) is the key protein in regulating heat stress response. [Method] The polymorphisms of HSF1 gene and their association with heat tolerance in 638 Chinese Holstein cattle were investigated for the purpose of providing molecular marker information to facilitate the breeding efficiency ofthermo tolerant cows.Primers were designed to amplify HSF1 gene fragment. DNA sequencing, PCR-SSCP and PCR-RFLP methods were used to analyze the polymorphisms. MicroRNA SNPs of HSF1 gene were localized by blasting to miRBASE database. Linkage disequilibrium and haplotype were analyzed using SHEsis software. The association of these polymorphisms with heat tolerance index including potassium content in erythrocytes (PCE), decrease rate of milk production (R), rectal temperature (RT) and heat-tolerance coefficient (HTC) was analyzed by SAS software. The transcriptional level of HSF1 mRNA under heat stress conditions was studied by fluorescent quantitative RT-PCR technology. [Result] Two novel microRNA SNPs, T909C and G4693T, were found. The cows with CC and CT genotype showed higher heat tolerance than those with TT genotype at T909C locus (P< 0.05). The cows with TT genotype showed higher heat tolerance than those with GG genotype at G4693T locus (P<0.05). Four haplotype and 10 haplotype combinations were found. The cows with H2H4 haplotype combination had lower PCE, RT and higher HCT than those with H1H3 haplotype combination (P<0.05). The cows with H2H4 haplotype combination had lower R than those with H1H1 haplotype combination (P<0.05). H2H4 was the heat tolerant haplotype combination. The transcriptional level of HSF1 mRNA under heat stress conditions was different in different tissues. The highest is that in heart, which is 11.24-fold enrichment than that in muscle (P<0.05). [Conclusion] microRNA SNPs of HSF1 gene could be chosen as the molecular markers for choosing thermo tolerant dairy cattle in breeding program

  8. The wing in yeast heat shock transcription factor (HSF) DNA-binding domain is required for full activity

    Cicero, Marco P.; T. Hubl, Susan; Harrison, Celia J.; Littlefield, Otis; Hardy, Jeanne A.; Nelson, Hillary C. M.

    2001-01-01

    The yeast heat shock transcription factor (HSF) belongs to the winged helix family of proteins. HSF binds DNA as a trimer, and additional trimers can bind DNA co-operatively. Unlike other winged helix–turn–helix proteins, HSF’s wing does not appear to contact DNA, as based on a previously solved crystal structure. Instead, the structure implies that the wing is involved in protein–protein interactions, possibly within a trimer or between adjacent trimers. To unders...

  9. HSF1生理特征及调控HSP表达的研究进展%Progress in the Physical Character and Regulation of HSF1

    徐雪; 马中富; 黄帆

    2008-01-01

    热休克因子1(the heat shock factorl,HSF1)通过与热休克蛋白基因上游的热休克元件相结合而调控热休克蛋白的表达,保护机体免受应激因素损害.其活化受到理化因素、细胞因子等不同水平机制的调控,具有结构、功能、活化以及调控过程的自身特点.

  10. Hsf1对人肝癌细胞株PLC/PRF5的生长调控作用%Regulatory fe fect of Hsf1 on PLC/PRF5 hepa toma cells proliferation

    蒋杞英; 张智; 王明丽; 马远方

    2014-01-01

    目的:探讨热休克转录因子1(Heat shock transcription factor 1,Hsf1)对人肝癌细胞株PLC/PRF5生长的调控作用。方法:通过shRNA基因沉默技术,构建 Hsf1基因沉默的PLC/PRF5肝癌细胞株。采用 Western blot检测PLC/PRF5肝癌细胞Hsf 1、p53和Rb蛋白的表达。通过四甲基偶氮唑盐( Methylthiazolyl tetrazolium assay ,MTT)法、平板克隆实验和细胞周期的检测,观察PLC/PRF5细胞株的增殖情况。结果:shRNA-Hsf1能有效地沉默Hsf1在PLC /PRF5细胞中的表达;shRNA-Hsf1能有效阻滞细胞周期于G1期,抑制PLC/PRF5细胞的生长速度和细胞克隆形成率;Hsf1基因沉默可上调PLC/PRF5细胞p53和Rb蛋白的表达。结论:Hsf1基因沉默可通过上调p53和Rb蛋白的表达抑制肝癌细胞株PLC/PRF5的增殖。%Objective:To explore the regulatory effect of Hsf 1 on PLC/PRF5 hepatoma cells proliferation.Methods: By shRNA gene silencing technology ,constructed PLC/PRF5 hepatoma cell line of Hsf 1 gene silencing.To detect the expression of Hsf 1, p53 and Rb proteins in PLC/PRF5 hepatoma cells by Western blot.The proliferation of PLC/PRF5 cell line was observed by methylthiazolyl tetrazolium assay ( MTT ) , plate clone formation assay ( PCFA ) and cell cycle assay.Results: shRNA-Hsf1 could significantly inhibit the expression of Hsf 1 in PLC/PRF5 cells.It could induce PLC/PRF5 cells stopping at G1 phase of cell cycle , inhibit cell proliferation and colonal formation;silencing Hsf1 caused up-regulation of p53 and Rb proteins expression in PLC/PRF5 cells.Conclusion: Silencing Hsf1 is involved in up-regulation of p53 and Rb proteins expression , which results in inhibiting proliferation of PLC/PRF5 hepatoma cells.

  11. The progress in the study of Arctic pack ice ecology

    何剑锋; 王桂忠; 蔡明红; 李少菁

    2004-01-01

    The sea ice community plays an important role in the Arctic marine ecosystem. Because of the predicted environmental changes in the Arctic environment and specifically related to sea ice, the Arctic pack ice biota has received more attention in recent years using modern ice-breaking research vessels. Studies show that the Arctic pack ice contains a diverse biota and besides ice algae, the bacterial and protozoan biomasses can be high. Surprisingly high primary production values were observed in the pack ice of the central Arctic Ocean. Occasionally biomass maximum were discovered in the interior of the ice floes, a habitat that had been ignored in most Arctic studies. Many scientific questions, which deserve special attention, remained unsolved due to logistic limitations and the sea ice characteristics. Little is know about the pack ice community in the central Arctic Ocean. Almost no data exists from the pack ice zone for the winter season. Concerning the abundance of bacteria and protozoa, more studies are needed to understand the microbial network within the ice and its role in material and energy flows. The response of the sea ice biota to global change will impact the entire Arctic marine ecosystem and a long-term monitoring program is needed. The techniques, that are applied to study the sea ice biota and the sea ice ecology, should be improved.

  12. Identifying Ecological Red Lines: A Case Study of the Coast in Liaoning Province

    Chuansheng Wang

    2015-07-01

    Full Text Available The global decline in estuarine and coastal ecosystems is affecting critical ecosystem services. The spatial agglomeration of population, industries and resources has led to the emergence of regionally-specific ecological problems. Therefore, identifying “ecological red lines”, based on specific natural and environmental features, could help to differentiate the economic development and ecological protection directions or potentials of different regions in future. The aim of this case study is to define the ecological red line in the coastal zone of Liaoning Province, China, by evaluating the ecological importance and environmental stress in its marine and terrestrial ecosystems. For this purpose, the ecological importance of this area was first classified into four conservation indices (species, wetland, water and coast and islands and assigned values of 5, 3 and 1 for indications of high, moderate and minor importance. In the meantime, environmental stress was also classified into four indices (water environment, salinization, soil erosion and erosion of coasts and islands and assigned values of 5, 3 and 1 for indications of high, moderate and low stress, respectively. Then, based on an overlay analysis and evaluation of the above results, we defined two grades of ecological red line zones. Grade I ecological red line zones contain the areas with critical and diverse ecosystem services, areas of high importance for species conservation and nature reserves, as well as ecologically-vulnerable and sensitive areas. It is important in these areas to maintain the biological diversity and to improve the quality of the ecological environment, which should be strictly protected and explicitly controlled. Grade II ecological red line zones display areas with minimum requirements for maintaining the basic needs of a livable environment and human health, moderate to minor levels of ecological importance and high to moderate levels of environmental

  13. An Ecological Flood Control System in Phoenix Island of Huzhou, China: A Case Study

    Zhuowen Wang

    2013-09-01

    Full Text Available Traditional flood control systems always have a conflict with natural ones, i.e., rivers in cities are usually straight and smooth, whereas natural ones are according to ecological mechanisms. Social and economic developments in the modern world require a new system combining ecological needs and traditional flood control system. Ecological flood control systems were put forward and defined as flood control systems with full consideration of ecological demands for sustainable development. In such systems, four aspects are promoted: connectivity of water system, landscapes of river and lakes, mobility of water bodies, and safety of flood control. In Phoenix Island, Huzhou, needs for ecological flood controls were analyzed from the four aspects above. The Water system layout was adjusted with the water surface ratio, which is the ratio of water surface area (including rivers, lakes, and other water bodies to the total drainage area, and connectivity as controlling indicators. The designed water levels provided references for landscape plant selection. Mobility of the adjusted water system was analyzed, including flow direction and residence time. On the bases mentioned above, ecological flood control projects were planned with comprehensive consideration of the ecological requirements. The case study indicates that ecological needs can be integrated with flood control to develop ecological flood control systems that do not only prevent floods but also retain the ecological functions of water bodies.

  14. Identifying Ecological Red Lines: A Case Study of the Coast in Liaoning Province

    Chuansheng Wang; Guiyan Sun; Lijuan Dang

    2015-01-01

    The global decline in estuarine and coastal ecosystems is affecting critical ecosystem services. The spatial agglomeration of population, industries and resources has led to the emergence of regionally-specific ecological problems. Therefore, identifying “ecological red lines”, based on specific natural and environmental features, could help to differentiate the economic development and ecological protection directions or potentials of different regions in future. The aim of this case study i...

  15. MEDIA, ECOLOGY AND WILDLIFE CONSERVATION: AN ANALYTICAL RESEARCH STUDY OF DUDHWA NATIONAL PARK

    Anurag Swaroop; M. K. Padhy

    2015-01-01

    Ecology is a science of ecosystems or totality of reciprocal interactions between living organisms and their physical surroundings (Clark 1973). The word ecology signifies the study of environments: their structure, content, and impact on people. Ecology is theoretically an academic discipline, such as mathematics or physics. The word wildlife is usually associated with non-domesticated vertebrates, but has broadly related to all wild animals, plants and other organisms. People from diverse f...

  16. Temporal and spatial melanoma trends in Austria: an ecological study.

    Haluza, Daniela; Simic, Stana; Moshammer, Hanns

    2014-01-01

    Annual solar ultraviolet radiation (UVR) is mostly determined by latitude and altitude. Over the last decades, increasing UVR ground levels have been observed. Exposure to UVR is associated with a life-time risk to develop melanoma, a malign skin cancer. Thus, we hypothesized that melanoma incidence in Austria is associated with altitude of place of living and time of diagnosis. We investigated this hypothesis in an ecological study by district and year for Austrian melanoma incidence (1990-2010) and mortality (1970-2011) data. As expected, incidence rates increased with altitude (about 2% per 10 m) and year (about 2%). Additionally, melanoma incidence rates were about 50% higher in urban than in rural districts. In contrast, mortality rates decreased with altitude (for males: 0.4% per 10 m, for women: 0.7% per 10 m, respectively). The observed discrepancy between incidence and mortality data could partly be explained by melanoma diagnosis at earlier tumor stage in districts with higher altitude. Possible reasons for this finding include higher awareness of patients, better diagnostic performance of medical professionals working at higher altitudes, or slower tumor growth due to protective effects of sun light-associated vitamin D synthesis. PMID:24398911

  17. Temporal and Spatial Melanoma Trends in Austria: An Ecological Study

    Daniela Haluza

    2014-01-01

    Full Text Available Annual solar ultraviolet radiation (UVR is mostly determined by latitude and altitude. Over the last decades, increasing UVR ground levels have been observed. Exposure to UVR is associated with a life-time risk to develop melanoma, a malign skin cancer. Thus, we hypothesized that melanoma incidence in Austria is associated with altitude of place of living and time of diagnosis. We investigated this hypothesis in an ecological study by district and year for Austrian melanoma incidence (1990–2010 and mortality (1970–2011 data. As expected, incidence rates increased with altitude (about 2% per 10 m and year (about 2%. Additionally, melanoma incidence rates were about 50% higher in urban than in rural districts. In contrast, mortality rates decreased with altitude (for males: 0.4% per 10 m, for women: 0.7% per 10 m, respectively. The observed discrepancy between incidence and mortality data could partly be explained by melanoma diagnosis at earlier tumor stage in districts with higher altitude. Possible reasons for this finding include higher awareness of patients, better diagnostic performance of medical professionals working at higher altitudes, or slower tumor growth due to protective effects of sun light-associated vitamin D synthesis.

  18. Study on the Programming Standard for Ecological Rescue in General Land Use Planning

    Bai Wei; Hao Jinmin; Zhang Qiuping

    2006-01-01

    This paper aims at establishing the operation idea based on the analysis of the connotation, principles and reference indexes for programming standard for ecological rescue. The paper puts forward that the programming standard should take into account the natural, social and economic reference indexes, and modifies the scale and distribution of the ecological rescue according to the order of ecological safety, social safety and economic development. The paper suggests that the land planning department should strengthen the study and datum accumulation in order to establish the technology regulations of programming standard of the ecological rescue.

  19. mTOR is essential for the proteotoxic stress response, HSF1 activation and heat shock protein synthesis.

    Shiuh-Dih Chou

    Full Text Available The target of rapamycin (TOR is a high molecular weight protein kinase that regulates many processes in cells in response to mitogens and variations in nutrient availability. Here we have shown that mTOR in human tissue culture cells plays a key role in responses to proteotoxic stress and that reduction in mTOR levels by RNA interference leads to increase sensitivity to heat shock. This effect was accompanied by a drastic reduction in ability to synthesize heat shock proteins (HSP, including Hsp70, Hsp90 and Hsp110. As HSP transcription is regulated by heat shock transcription factor 1 (HSF1, we examined whether mTOR could directly phosphorylate this factor. Indeed, we determined that mTOR could directly phosphorylate HSF1 on serine 326, a key residue in transcriptional activation. HSF1 was phosphorylated on S326 immediately after heat shock and was triggered by other cell stressors including proteasome inhibitors and sodium arsenite. Null mutation of S326 to alanine led to loss of ability to activate an HSF1-regulated promoter-reporter construct, indicating a direct role for mTOR and S326 in transcriptional regulation of HSP genes during stress. As mTOR is known to exist in at least two intracellular complexes, mTORC1 and mTOR2 we examined which complex might interact with HSF1. Indeed mTORC1 inhibitor rapamycin prevented HSF1-S326 phosphorylation, suggesting that this complex is involved in HSF1 regulation in stress. Our experiments therefore suggest a key role for mTORC1 in transcriptional responses to proteotoxic stress.

  20. Cloning and Sequence Analysis of HSF1 cDNA Full Length of Hainan Yellow Cattle%海南黄牛HSF1 cDNA全长的克隆与序列分析

    成鹰; 林杰材; 满初日嘎; 米华存; 祁超; 李治深; 杜丽; 王凤阳; 刘涛; 申明霞; 张莉娜; 张巍; 吴科榜

    2008-01-01

    目的:根据人、小鼠HSF1cDNA保守区序列设计引物,通过PCR方法扩增海南黄牛HSF1cDNA,并进行序列分析.方法:利用RT-PER、半巢式PCR以及3'-RACE技术分段扩增得到了海南黄牛HSF1cDNA序列,测序正确后进行拼接.用DNANAN生物信息学软件分析海南黄牛HSF1cDNA与赫里福德牛、人、小鼠同源性和海南黄牛HSF1蛋白的氨基酸组成、等电点、亲/疏水区等蛋白质性质,并根据各种动物HSF1蛋白绘制进化树.结果:(1)海南黄牛的HSF1 cDNA序列全长为1 993bp,包括150bp的5'非翻译区,1 578bp的开放阅读框以及264bp(不含poly(A)尾)的3'非翻译区,编码524个氨基酸,分子量为56.42 kD,等电点(pI)为4.79.(2)海南黄牛的HSF1 cDNA与赫里福德牛、小鼠和人HSF1 cDNA的同源性分别为98.99%、81.78%、87.82%,相应编码蛋白氨基酸序列的同源性分别为98.86%、83.84%、89.06%,其中N-末端和C-末端高度保守,而中间区域存在缺失或替换.(3)根据氨基酸序列构建不同动物HSF1蛋白的进化树,与采用经典遗传分类法构建的进化树基本一致.结论:首次克隆了海南黄牛HSF1 cDNA全长,分析表明:海南黄牛HSF1蛋白是亲水性蛋白,在8种动物中,其同源性大于73%,高度保守.海南黄牛与赫里福德牛HSF1蛋白同源性高达98.86%,在三聚体化区域、转录调节域和激活域存在6个位点的单氨基酸突变,这些发现为进一步揭示海南黄牛抗热性状形成的分子机制提供了重要依据.

  1. Site-Specific ecological risk assessment. Case-study 2

    Jensen, John

    The decision supporting and integrating assessment tool, TRIAD, is used site-specific on PAH- and heavy metal contaminated sites in Denmark. The various aspects of the TRIAD approach are used on a set of chemistry-, ecotoxicology- and ecology related data collected among others in the EU project...... “Development of a decision support system for sustainable management of contaminated land by linking bioavailability, ecological risk and ground water pollution of organic pollutants”or in short “LIBERATION”. The presentation includes examples on how to scale and integrate the results from various scientific...

  2. 前列腺癌中HSF1蛋白的表达及与临床病理特征的相关性%Correlation Between Expression of HSF1 and Clinicopathological Features of Prostate Cancer

    张晓波; 齐琳; 唐正严; 阮景德

    2010-01-01

    目的 探讨HSF1在前列腺癌组织中的表达及与前列腺癌各项临床病理特征的关系.方法 应用免疫组织化学法和图像分析系统研究82例前列腺癌标本中HS目的 表达情况.结果 HSF1蛋白在前列腺癌癌细胞胞浆部化染色阳性,阳性表达率为89.2%.HSF1在前列腺癌组织中的表达与Gleason评分呈正相关(r=0.66,P<0.001).HSF1表达与PSA水平随PSA升高而升高,呈正相关(r=0.76,P<0.001).结论 HSF1蛋白在前列腺癌中表达增加,可能与调控热休克蛋白家族成员作用机制有关.

  3. PCR方法在HSF1基因敲除小鼠基因型分析中的应用%Genotype Analysis of HSF1 Gene Knockout Mice with Polymerase Chain Reaction

    陈广文; 刘喜玲; 肖献忠

    2002-01-01

    目的为HSF1基因敲除鼠探索快速、简单的基因型PCR检测方法.方法设计两对引物扩增野生型HSF1基因和HSF1缺陷突变基因的DNA片段,用PCR仪梯度方案测试最佳退火温度,并将所得基因型结果与经典的Southern blot方法比较.结果野生型仅在562 bp处有一条条带,突变纯合子仅在377 bp处有一条条带,杂合子则在377 bp和562 bp处出现两条条带. 用PCR方法获得的HSF1基因分析结果与经典的Southern blot方法获得的结果完全一致.结论用PCR方法分析HSF1基因敲除鼠的基因型具有快速、简单、廉价和适用的特点.

  4. 双荧光素酶报告质粒检测小鼠巨噬细胞热休克转录因子1调控核转录因子kappa B活性的研究%A Study of the Effects of HSF1 on NF-κB Activity in RAW264.7 Macrophages by Dual-luciferase Assay

    李雄; 罗成群; 周建大; 贺全勇; 徐阳成

    2010-01-01

    目的:观察烧伤血清刺激后小鼠巨噬细胞NF-κB活性的变化,以及HSF1对NF-κB可能的调控作用.方法:制作15%TBSA Ⅲ°烧伤小鼠模型,提取烧伤血清.通过表达质粒与报告质粒共转染,检测烧伤血清诱导下NF-κB活性的变化以及过表达HSFI后NF-κB活性的变化规律.结果:对比正常血清,烧伤血清刺激后相对荧光素酶活性早期即明显增加(P<0.05),这种变化在诱导后2 h即达到高峰,12 h后逐渐下降;过表达HSF1可以显著抑制烧伤血清引起这种活性变化(P<0.05).结论:烧伤后NF-κB早期即活化,热休克反应可能通过HSF1途径抑制NF-κB的活性.

  5. Estimating bacterial diversity for ecological studies: methods, metrics, and assumptions.

    Julia Birtel

    Full Text Available Methods to estimate microbial diversity have developed rapidly in an effort to understand the distribution and diversity of microorganisms in natural environments. For bacterial communities, the 16S rRNA gene is the phylogenetic marker gene of choice, but most studies select only a specific region of the 16S rRNA to estimate bacterial diversity. Whereas biases derived from from DNA extraction, primer choice and PCR amplification are well documented, we here address how the choice of variable region can influence a wide range of standard ecological metrics, such as species richness, phylogenetic diversity, β-diversity and rank-abundance distributions. We have used Illumina paired-end sequencing to estimate the bacterial diversity of 20 natural lakes across Switzerland derived from three trimmed variable 16S rRNA regions (V3, V4, V5. Species richness, phylogenetic diversity, community composition, β-diversity, and rank-abundance distributions differed significantly between 16S rRNA regions. Overall, patterns of diversity quantified by the V3 and V5 regions were more similar to one another than those assessed by the V4 region. Similar results were obtained when analyzing the datasets with different sequence similarity thresholds used during sequences clustering and when the same analysis was used on a reference dataset of sequences from the Greengenes database. In addition we also measured species richness from the same lake samples using ARISA Fingerprinting, but did not find a strong relationship between species richness estimated by Illumina and ARISA. We conclude that the selection of 16S rRNA region significantly influences the estimation of bacterial diversity and species distributions and that caution is warranted when comparing data from different variable regions as well as when using different sequencing techniques.

  6. Aggregation of polyQ proteins is increased upon yeast aging and affected by Sir2 and Hsf1: novel quantitative biochemical and microscopic assays.

    Aviv Cohen

    Full Text Available Aging-related neurodegenerative disorders, such as Parkinson's, Alzheimer's and Huntington's diseases, are characterized by accumulation of protein aggregates in distinct neuronal cells that eventually die. In Huntington's disease, the protein huntingtin forms aggregates, and the age of disease onset is inversely correlated to the length of the protein's poly-glutamine tract. Using quantitative assays to estimate microscopically and capture biochemically protein aggregates, here we study in Saccharomyces cerevisiae aging-related aggregation of GFP-tagged, huntingtin-derived proteins with different polyQ lengths. We find that the short 25Q protein never aggregates whereas the long 103Q version always aggregates. However, the mid-size 47Q protein is soluble in young logarithmically growing yeast but aggregates as the yeast cells enter the stationary phase and age, allowing us to plot an "aggregation timeline". This aging-dependent aggregation was associated with increased cytotoxicity. We also show that two aging-related genes, SIR2 and HSF1, affect aggregation of the polyQ proteins. In Δsir2 strain the aging-dependent aggregation of the 47Q protein is aggravated, while overexpression of the transcription factor Hsf1 attenuates aggregation. Thus, the mid-size 47Q protein and our quantitative aggregation assays provide valuable tools to unravel the roles of genes and environmental conditions that affect aging-related aggregation.

  7. Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades

    Masako Nakamura

    2012-02-01

    Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR. Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2 conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression.

  8. Ecological studies on rain forest in Northern Suriname

    Schulz, J.P.

    1960-01-01

    During the years 1955-1957 ecological data were collected in various types of mesophytic forest occurring in the northern half of central Suriname (fig. 1). Physiognomically as well as floristically these forests correspond with the type of vegetation which in the other parts of tropical America gen

  9. Study on remote sensing method for drawing up and utilizing ecological and natural map II; concentrated on drawing up a plant ecological classification map

    Jeon, Seong Woo; Chung, Hwui Chul [Korea Environment Institute, Seoul (Korea)

    1999-12-01

    Following with the flows of the environmental conservation, Korea has revised the law of natural environmental conservation. In this law, it has suggested to draw up an ecological nature figure for efficient preservation and utilization of a country. To draw up an ecological nature figure, it requires several evaluating factors. Among them, a plant ecological classification is a very important evaluating factor since it can evaluate a habitation area of natural organisms. This study investigated a drawing up method of plant ecological classification using satellite image data. However the limit of satellite image data and the quality of required plant ecological classification are not quite matched but if the satellite image data and the infrared color aerial photograph are mixed, it can be expected to have an excellent quality of plant ecological classification. 85 refs., 86 figs., 45 tabs.

  10. HSF1中的半胱氨酸残基氧化还原状态对其功能的调节%HSF1 may have Integrated Redox Dependent Regulation of Cysteine Residues into Its Function Response

    林正; 黄帆; 罗兰; 张式鸿; 马中富; 吴兴刚; 徐康

    2005-01-01

    人热休克转录因子1(heat shock transcription factor l,HSF1)的结构和功能与其半胱氨酸残基的氧化还原化学性能相关.为了鉴别在氧化还原状态改变时参与分子内双硫键交联的HSF1半胱氨酸残基,了解其氧化还原化学性改变在生物学调节中的重要性,通过建立和应用重组人HSF1中的5个半胱氨酸突变体和1个双重突变体,并用已知的巯基氧化介导剂联氨(diamide,H2N·NH2)和还原介导剂二硫苏糖醇(DTT)与在体外转录和翻译的HSF1突变体蛋白质预孵育,观察其构象和与DNA结合活性的改变.结果显示,与野生型一样,所有的HSF1半胱氨酸突变体都能被热激活并与DNA结合;联氨预处理能阻断这种作用,但对突变体C153S和双重突变体G373、378S无效.氧化还原状态对HSF1构象改变显示联氨能使HSF1野生型和突变体C36T和C103Y形成氧化型HSF1(ox-HSF1)构象,但对C153S和C373、378S双重突变体不起作用,而单一突变体C373S或C378S在联氨作用下分别形成二种分子量稍不同的ox-HSF1构象.结果提示,在氧化条件下HSF1中的半胱氨酸残基C153可能与C373或与G378形成分子内二硫键交联;在对抗氧化作用上5C153和C373、C378起着"关闭性"作用,预防了HSF1的激活.

  11. Political Cultural Ecology and the Study of Regions in Mexico

    Fábregas Puig, Andrés

    2009-01-01

    This paper shows why Cultural Ecology, understood as a method to explore the relationships between the environment and cultural processes, has deeply influenced a whole generation of Mexican anthropologists as a result of the teachings of Ángel Palerm and Eric Wolf. The practical application of Steward’s theories to the processes of Mexican regional formation, linked to the use of hydraulic technologies as part of both adaptative cultural strategies and the political control of water in the p...

  12. CASE STUDY: Community Based Ecological Mangrove Rehabilitation (CBEMR) in Indonesia

    Brown, Ben; Fadillah, Ratna; Nurdin, Yusran; Soulsby, Iona; Ahmad, Rio

    2014-01-01

    While successful examples of large-scale (5 000-10 000 ha) ecological wetland/mangrove rehabilitation projects exist worldwide, mangrove rehabilitation efforts in Indonesia, both large and small, have mainly failed. The majority of projects (both government programs and non-government initiatives) have oversimplified the technical processes of mangrove rehabilitation, favouring the direct planting of a restricted subset of mangrove species (from the family Rhizophoracea), commonly in the lowe...

  13. Ecological Studies On The Bottom Fauna Of Lake Manala, Egypt

    Khalil, Magdy T. [مجدي توفيق خليل

    1990-01-01

    An ecological survey has been conducted for the benthic Community in Lake Manzala during May and June, 1985. The diversity and distribution of organisms are largely determined by salinity. Mean abundance of benthic fauna ranged from 1494 to 2820 organisms/m2 for sampling transects. Pisidium and Melanoides mollusca dominate the low salinity southern regions, while Cerastoctenna, Abra and Alvania species dominate the saline sectors. Two other species of considerable importance in the lake ...

  14. Ecological niche transferability using invasive species as a case study.

    Miguel Fernández

    Full Text Available Species distribution modeling is widely applied to predict invasive species distributions and species range shifts under climate change. Accurate predictions depend upon meeting the assumption that ecological niches are conserved, i.e., spatially or temporally transferable. Here we present a multi-taxon comparative analysis of niche conservatism using biological invasion events well documented in natural history museum collections. Our goal is to assess spatial transferability of the climatic niche of a range of noxious terrestrial invasive species using two complementary approaches. First we compare species' native versus invasive ranges in environmental space using two distinct methods, Principal Components Analysis and Mahalanobis distance. Second we compare species' native versus invaded ranges in geographic space as estimated using the species distribution modeling technique Maxent and the comparative index Hellinger's I. We find that species exhibit a range of responses, from almost complete transferability, in which the invaded niches completely overlap with the native niches, to a complete dissociation between native and invaded ranges. Intermediate responses included expansion of dimension attributable to either temperature or precipitation derived variables, as well as niche expansion in multiple dimensions. We conclude that the ecological niche in the native range is generally a poor predictor of invaded range and, by analogy, the ecological niche may be a poor predictor of range shifts under climate change. We suggest that assessing dimensions of niche transferability prior to standard species distribution modeling may improve the understanding of species' dynamics in the invaded range.

  15. Studies on marine oil spills and their ecological damage

    Mei, Hong; Yin, Yanjie

    2009-09-01

    The sources of marine oil spills are mainly from accidents of marine oil tankers or freighters, marine oil-drilling platforms, marine oil pipelines, marine oilfields, terrestrial pollution, oil-bearing atmosphere, and offshore oil production equipment. It is concluded upon analysis that there are two main reasons for marine oil spills: (I) The motive for huge economic benefits of oil industry owners and oil shipping agents far surpasses their sense of ecological risks. (II) Marine ecological safety has not become the main concern of national security. Oil spills are disasters because humans spare no efforts to get economic benefits from oil. The present paper draws another conclusion that marine ecological damage caused by oil spills can be roughly divided into two categories: damage to marine resource value (direct value) and damage to marine ecosystem service value (indirect value). Marine oil spills cause damage to marine biological, fishery, seawater, tourism and mineral resources to various extents, which contributes to the lower quality and value of marine resources.

  16. Studies on Marine Oil Spills and Their Ecological Damage

    MEI Hong; YIN Yanjie

    2009-01-01

    The sources of marine oil spills are mainly from accidents of marine oil tankers or freighters, marine oil-drilling platforms, marine oil pipelines, marine oilfields, terrestrial pollution, oil-bearing atmosphere, and offshore oil production equipment. It is concluded upon analysis that there are two main reasons for marine oil spills: (Ⅰ) The motive for huge economic benefits of oil Industry owners and oil shipping agents far surpasses their sense of ecological risks. (Ⅱ) Marine ecological safety has not become the main concern of national security. Oil spills are disasters because humans spare no efforts to get economic benefits from oil. The present paper draws another conclusion that marine ecological damage caused by oil spills can be roughly divided into two categories: damage to marine resource value (direct value) and damage to marine ecosystem service value (indirect value). Marine oil spills cause damage to marine biological, fishery, seawater, tourism and mineral resources to various extents, which contributes to the lower quality and value of marine resources.

  17. Ecological niche transferability using invasive species as a case study.

    Fernández, Miguel; Hamilton, Healy

    2015-01-01

    Species distribution modeling is widely applied to predict invasive species distributions and species range shifts under climate change. Accurate predictions depend upon meeting the assumption that ecological niches are conserved, i.e., spatially or temporally transferable. Here we present a multi-taxon comparative analysis of niche conservatism using biological invasion events well documented in natural history museum collections. Our goal is to assess spatial transferability of the climatic niche of a range of noxious terrestrial invasive species using two complementary approaches. First we compare species' native versus invasive ranges in environmental space using two distinct methods, Principal Components Analysis and Mahalanobis distance. Second we compare species' native versus invaded ranges in geographic space as estimated using the species distribution modeling technique Maxent and the comparative index Hellinger's I. We find that species exhibit a range of responses, from almost complete transferability, in which the invaded niches completely overlap with the native niches, to a complete dissociation between native and invaded ranges. Intermediate responses included expansion of dimension attributable to either temperature or precipitation derived variables, as well as niche expansion in multiple dimensions. We conclude that the ecological niche in the native range is generally a poor predictor of invaded range and, by analogy, the ecological niche may be a poor predictor of range shifts under climate change. We suggest that assessing dimensions of niche transferability prior to standard species distribution modeling may improve the understanding of species' dynamics in the invaded range. PMID:25785858

  18. Assessing Landscape Ecological Risk in a Mining City: A Case Study in Liaoyuan City, China

    Jian Peng

    2015-06-01

    Full Text Available Landscape ecological risk assessment can effectively identify key elements for landscape sustainability, which directly improves human wellbeing. However, previous research has tended to apply risk probability, measured by overlaying landscape metrics to evaluate risk, generally lacking a quantitative assessment of loss and uncertainty of risk. This study, taking Liaoyuan City as a case area, explores landscape ecological risk assessment associated with mining cities, based on probability of risk and potential ecological loss. The assessment results show landscape ecological risk is lower in highly urbanized areas than those rural areas, suggesting that not only cities but also natural and semi-natural areas contribute to overall landscape-scale ecological risk. Our comparison of potential ecological risk in 58 watersheds in the region shows that ecological loss are moderate or high in the 10 high-risk watersheds. The 35 moderate-risk watersheds contain a large proportion of farmland, and the 13 low-risk watersheds are mainly distributed in flat terrain areas. Our uncertainty analyses result in a close range between simulated and calculated values, suggesting that our model is generally applicable. Our analysis has good potential in the fields of resource development, landscape planning and ecological restoration, and provides a quantitative method for achieving landscape sustainability in a mining city.

  19. Hsf1-潜在的睾丸支持细胞雄激素受体靶基因%Hsf1 as a potential target of androgen/AR in mouse sertoli cells

    王亚东; 叶炯贤; 牟丽莎; 来永庆; 李贤新; 桂耀庭

    2013-01-01

    目的 探讨雄激素受体(AR)对睾丸支持细胞中热休克转录因子1(Hsf1)基因表达的影响及其分子机制.方法 采用PCR法及Western blot法检测,AR特异性敲除(S-AR-/y)小鼠和野生型(WT)小鼠睾丸组织中Hsf1的表达,观察雄激素对TM4细胞系中Hsf1和热休克蛋白(HSPs)表达的影响.结果 与WT小鼠比较,S-AR-/y小鼠睾丸组织中Hsf1表达水平升高(P<0.05);雄激素显著降低TM4细胞中热休克转录因子-1(HSF1)表达(P<0.05),并且HSF1能够升高热休克蛋白105(HSP105)和HSP60水平.结论 Hsf1可能是睾丸支持细胞中AR调控的靶基因.%Objective To identify the heat shook transcription factor-l( Hsfl ) as a target gene of AR in sertoli cells and its molecular mechanism in male infertility. Methods PCR and Western blot was used to compare the expression level of Hsfl in sertoli cell-selective knockout AR mice ( S-AR-/y ) with wild type mice ( WT ) and TM4 cell line with or without testosterone. Results Hsfl expression level in S-AR-/y mice was significantly increased compared with WT mice. On condition of testosterone, Hsfl expression of TM4 cell line was significantly reduced in both mRNA and protein level. Moreover, our data suggested that HSF1 acted as a positive factor for target gene expression, in particular regulating heat shock protein 105 ( HSP105 ) and HSP60. Conclusion The Hsfl may plays as a novel target gene of AR in mice sertoli cells.

  20. Ecological responses to contamination: a meta-analysis of experimental marine studies.

    O'Brien, Allyson L; Keough, Michael J

    2014-12-01

    Identifying general response patterns to contamination in the environment is critical for reliable assessments of ecosystem health. However, it is not often clear if there are biases in the information used to understand general effects of contamination. To investigate this we conducted a review of 314 studies that experimentally manipulated contaminants and measured the effects on marine invertebrate taxa. The majority of studies investigated the effects of metals (54%) on individual taxa (mainly bivalves, amphipods, copepods). Ecologically relevant responses to contamination were measured in only 22% of the studies. A meta-analysis using studies that measured ecological responses to copper illustrated a general negative effect of copper and highlighted the bias towards field or laboratory experiments that measure community or individual-level responses. There is a need for diversification of studies that investigate the ecological effects of contamination as an important advancement in ecotoxicology and ecological research and environmentally relevant risk assessments. PMID:25247875

  1. HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation

    Andrea B. Zepeda

    2014-06-01

    Full Text Available Pichia pastoris is a methylotrophic yeast used as an efficient expression system for heterologous protein production as compared to other expression systems. Considering that every cell must respond to environmental changes to survive and differentiate, determination of endogenous protein related to heat stress responses and hypoxia, it would necessary to establish the temperature and methanol concentration conditions for optimal growth. The aim of this study is characterize the culture conditions through the putative biomarkers in different conditions of temperature and methanol concentration. Three yeast cultures were performed: 3X = 3% methanol -10 °C, 4X = 3% methanol -30 °C, and 5X = 1% methanol -10 °C. The expression level of HIF-1α, HSF-1, HSP-70 and HSP-90 biomarkers were measured by Western blot and in situ detection was performed by immunocytochemistry. The western blot results of HIF-1α and HSP-90 did not indicate statistically significant in the culture conditions studied. Respect to biomarkers location, HIF-1α and HSP-90 presented differences between cultures. In conclusion, the results suggest the cultures in a hypoxic condition produce a high density and yeast cells smaller. Beside the high density would not necessary related with a high production of recombinant proteins in modified-genetically P. pastoris.

  2. Amchitka aquatic ecology studies, third quarter, January through March 1968: Progress report

    US Fish and Wildlife Service, Department of the Interior — The objectives of the aquatic ecology study include an assessment of the fish and aquatic invertebrate populations in the streams and in the ponds throughout the...

  3. A holistic approach to studying social-ecological systems and its application to southern Transylvania

    Jan Hanspach; Tibor Hartel; Andra I. Milcu; Friederike Mikulcak; Ine Dorresteijn; Jacqueline Loos; Henrik von Wehrden; Tobias Kuemmerle; David Abson; Anikó Kovács-Hostyánszki; András Báldi; Joern Fischer

    2014-01-01

    Global change presents risks and opportunities for social-ecological systems worldwide. Key challenges for sustainability science are to identify plausible future changes in social-ecological systems and find ways to reach socially and environmentally desirable conditions. In this context, regional-scale studies are important, but to date, many such studies have focused on a narrow set of issues or applied a narrow set of tools. Here, we present a holistic approach to work through the complex...

  4. Applications of C and N stable isotopes to ecological and environmental studies in seagrass ecosystems

    Lepoint, Gilles; Dauby, Patrick; Gobert, Sylvie

    2004-01-01

    Stable isotopes of carbon and nitrogen are increasingly used in marine ecosystems, for ecological and environmental studies. Here, we examine some applications of stable isotopes as ecological integrators or tracers in seagrass ecosystem studies. We focus on both the use of natural isotope abundance as food web integrators or environmental tracers and on the use of stable isotopes as experimental tools. As ecosystem integrators, stable isotopes have helped to elucidate the general structure o...

  5. Ecological studies related to construction of the Defense Waste Processing Facility on the Savannah River Site

    The Savannah River Ecology Laboratory has completed 10 years of ecological studies related to the construction of the Defense Waste Processing Facility (DWPF) on the Savannah River Site. This progress report examines water quality studies on streams peripheral to the DWPF construction site and examines the effectiveness of ''refuge ponds'' in ameliorating the effects of construction on local amphibians. Individual papers on these topics are indexed separately. 93 refs., 15 figs., 15 tabs

  6. Use of residence time models in ecological studies of transuranics

    The paper discusses the applicability of different types of ecological models. An explanation of the various concepts and the basis for residence time models and how they are correlated is presented. Mean residence time and pseudo residence time are terms useful for more complicated systems. The term chromatography defines a conceptual model, but the processes involved are limited to diffusion, dispersion, convection, adsorption and desorption. The most sophisticated type of models are the simulation models. Regression equations are used to calculate the transfer between pools of simulation models. However, simulation models consider processes in great detail and processes are described by appropriate equations. Lack of useful parameter values in the main limitation for applying such models. Their main value is in promoting understanding of radioecological processes and in identifying missing information. (author)

  7. The Effects of Materialism and Consumer Ethics on Ecological Behavior: An Empirical Study

    Hülya Bakırtaş

    2014-10-01

    Full Text Available World populations have increased day by day. Thus, production volume of companies have increased depend on increasing population. Besides, fashion and/or technologyinfluence consumer’s goods or services purchasing decision. Consequently, the type and the number of product produced have increased and natural resources both more are being used and depleted. At this point has come to the fore the consumer’s environmental concern and ecological behavior and has gained importance. Studies about environmentalconcern in marketing began depending on living of some problems in the early 1970s. Environmental concern concept assesses in terms of both individual concern and socialconcern. Individual concern states to abuse of the environment of individual consumers while social concern states perceived need for social, political, and legal changes to protect the environment. There is a positive relationship between environmental concern and ecological behavior. This study was examined the relationship between materialism,consumer ethics, environmental concern and ecological consumer behavior. This study was carried out between dates of September and November 2013. To test thehypotheses of the study, regression analysis has been used. Results show that consumer ethics has positive impact on both environmental concern and ecological behaviour ofconsumer. Besides, materialism has negative impact on ecological behaviour.Keywords: Environmental concern, consumer ethics, materialism, ecological behavior.

  8. Assessing Landscape Ecological Risk in a Mining City: A Case Study in Liaoyuan City, China

    Jian Peng; Minli Zong; Yi'na Hu; Yanxu Liu; Jiansheng Wu

    2015-01-01

    Landscape ecological risk assessment can effectively identify key elements for landscape sustainability, which directly improves human wellbeing. However, previous research has tended to apply risk probability, measured by overlaying landscape metrics to evaluate risk, generally lacking a quantitative assessment of loss and uncertainty of risk. This study, taking Liaoyuan City as a case area, explores landscape ecological risk assessment associated with mining cities, based on probability of ...

  9. Ecological speciation in the tropics: insights from comparative genetic studies in Amazonia.

    Beheregaray, Luciano B; Cooke, Georgina M; Chao, Ning L; Landguth, Erin L

    2014-01-01

    Evolution creates and sustains biodiversity via adaptive changes in ecologically relevant traits. Ecologically mediated selection contributes to genetic divergence both in the presence or absence of geographic isolation between populations, and is considered an important driver of speciation. Indeed, the genetics of ecological speciation is becoming increasingly studied across a variety of taxa and environments. In this paper we review the literature of ecological speciation in the tropics. We report on low research productivity in tropical ecosystems and discuss reasons accounting for the rarity of studies. We argue for research programs that simultaneously address biogeographical and taxonomic questions in the tropics, while effectively assessing relationships between reproductive isolation and ecological divergence. To contribute toward this goal, we propose a new framework for ecological speciation that integrates information from phylogenetics, phylogeography, population genomics, and simulations in evolutionary landscape genetics (ELG). We introduce components of the framework, describe ELG simulations (a largely unexplored approach in ecological speciation), and discuss design and experimental feasibility within the context of tropical research. We then use published genetic datasets from populations of five codistributed Amazonian fish species to assess the performance of the framework in studies of tropical speciation. We suggest that these approaches can assist in distinguishing the relative contribution of natural selection from biogeographic history in the origin of biodiversity, even in complex ecosystems such as Amazonia. We also discuss on how to assess ecological speciation using ELG simulations that include selection. These integrative frameworks have considerable potential to enhance conservation management in biodiversity rich ecosystems and to complement historical biogeographic and evolutionary studies of tropical biotas. PMID:25653668

  10. Spatial variation in lake benthic macroinvertebrate ecological assessment: a synthesis of European case studies

    Sandin, Leif Leonard; Solimini, Angelo G.

    2012-01-01

    This paper summarizes eight case studies that were analysed as part of the research theme ``lake benthic macroinvertebrates'' forming part of the EU-funded WISER project ``Water bodies in Europe: Integrative Systems to assess Ecological status and Recovery''. The relationships between lake benthi...... our aim is to provide useful information for designing monitoring programs and invertebrate based ecological classification tools with the ultimate aim to improve a sound management of European lake ecosystems....

  11. Affect Regulation and Purging: An Ecological Momentary Assessment Study in Purging Disorder

    Haedt-Matt, Alissa A.; Keel, Pamela K.

    2015-01-01

    Research suggests that affect may play an important role in the propensity to purge among women with Purging Disorder (PD). However, prior work has been constrained to cross-sectional or laboratory designs which impact temporal interpretations and ecological validity. This study examined the role of negative affect (NA) and positive affect (PA) in triggering and maintaining purging in PD using ecological momentary assessment. Women with PD (N=24) made multiple daily ratings of affect and beha...

  12. Ecological Optimization and Parametric Study of an Irreversible Regenerative Modified Brayton Cycle with Isothermal Heat Addition

    Vivek Tiwari; Subhash Chandra Kaushik; Sudhir Kumar Tyagi

    2003-01-01

    Abstract: An ecological optimization along with a detailed parametric study of an irreversible regenerative Brayton heat engine with isothermal heat addition have been carried out with external as well as internal irreversibilities. The ecological function is defined as the power output minus the power loss (irreversibility) which is ambient temperature times the entropy generation rate. The external irreversibility is due to finite temperature difference between the heat engine and the exter...

  13. Toward a Network Perspective of the Study of Resilience in Social-Ecological Systems

    Ryan R. J. McAllister

    2006-06-01

    Full Text Available Formal models used to study the resilience of social-ecological systems have not explicitly included important structural characteristics of this type of system. In this paper, we propose a network perspective for social-ecological systems that enables us to better focus on the structure of interactions between identifiable components of the system. This network perspective might be useful for developing formal models and comparing case studies of social-ecological systems. Based on an analysis of the case studies in this special issue, we identify three types of social-ecological networks: (1 ecosystems that are connected by people through flows of information or materials, (2 ecosystem networks that are disconnected and fragmented by the actions of people, and (3 artificial ecological networks created by people, such as irrigation systems. Each of these three archytypal social-ecological networks faces different problems that influence its resilience as it responds to the addition or removal of connections that affect its coordination or the diffusion of system attributes such as information or disease.

  14. The transcriptional coactivator PGC1α protects against hyperthermic stress via cooperation with the heat shock factor HSF1.

    Xu, L; Ma, X; Bagattin, A; Mueller, E

    2016-01-01

    Heat shock proteins (HSPs) are required for the clearance of damaged and aggregated proteins and have important roles in protein homeostasis. It has been shown that the heat shock transcription factor, HSF1, orchestrates the transcriptional induction of these stress-regulated chaperones; however, the coregulatory factors responsible for the enhancement of HSF1 function on these target genes have not been fully elucidated. Here, we demonstrate that the cold-inducible coactivator, PGC1α, also known for its role as a regulator of mitochondrial and peroxisomal biogenesis, thermogenesis and cytoprotection from oxidative stress, regulates the expression of HSPs in vitro and in vivo and modulates heat tolerance. Mechanistically, we show that PGC1α physically interacts with HSF1 on HSP promoters and that cells and mice lacking PGC1α have decreased HSPs levels and are more sensitive to thermal challenges. Taken together, our findings suggest that PGC1α protects against hyperthermia by cooperating with HSF1 in the induction of a transcriptional program devoted to the cellular protection from thermal insults. PMID:26890141

  15. The ecology of early farming: A Mogollon case study

    Swanson, Steve

    The Mimbres region of southwest New Mexico is ideal for investigating the coevolutionary relationships among human impact, ecosystem processes, and settlement stability during the development of sedentism and agriculture. A resilience framework guides interpretations of nuanced relations among land use strategies and impacts through an examination of ecosystem processes around Mimbres pithouse sites. Analytical methods include multitemporal analysis of satellite imagery to measure vegetation response to precipitation variability and GIS-based spatial analyses to examine differences in ecosystem response for prehistoric sites and adjacent unoccupied locations in similar ecological settings. Palynological and architectural analyses provide independent means of assessing prehistoric impact and settlement choices (respectively). To evaluate the relative contribution of prehistoric selection and impact on occupation length, models are developed for "choice"---ecosystem characteristics that attracted initial settlement, and "impact"---modification to ecosystems resulting from prehistoric land use. Choices reflecting productivity maximization find support; prehistoric impact played a smaller role. Pollen data suggest even small prehistoric occupations shifted plant communities towards r-strategist taxa, but impacts were not long-lived, and legacy effects of Mimbres land use on modern ecosystem processes may be minor. Some Mimbres settlements had large, long occupations (villages), but most settlements were small and only briefly occupied (farmsteads). Greater architectural investment in early houses at villages than at farmsteads suggests occupation length was planned during initial construction. Satellite data indicate locations for each type of settlement were selected based on the way vegetation responds to precipitation variability. Farmsteads were constructed where pluvial effects last longer, but the effects of drought are more severe. Shorter use of these

  16. Ecological Footprint of Research University Students: A Pilot Case Study in Universiti Teknologi Malaysia

    See Tan Ang

    2016-01-01

    Full Text Available Ecological footprint (EF is potential to be applied in universities to assist building management units to coordinate in order to reduce environmental impact and to achieve sustainable resource consumption from its main activities including teaching-learning, research and operations. As many Malaysian universities declare to become sustainability campus, the adoption of ecological footprint in measuring campus sustainability will provide insight and better understanding about the performance of campus sustainability efforts. The main concept of ecological footprint which convert levels of consumption into the amount of land needed, will able to reveal the average student performance and impacts towards the campus. Further, a study is conducted to determine the average ecological footprint level of students in research universities Malaysia considering students formed the majority of the community in a campus. A pilot study has been conducted in Universiti Teknologi Malaysia (UTM among students staying in hostel together with staffs from UTM Office of Student Affairs and Alumni (HEMA and UTM Office of Asset and Development (PHB. Then, Redefining Progress (RP ecological footprint online calculator is used in computing the ecological footprint of UTM students.

  17. Ecological risk assessment guidance for preparation of remedial investigation/feasibility study work plans

    This guidance document (1) provides instructions on preparing the components of an ecological work plan to complement the overall site remedial assessment investigation/feasibility study (RI/FS) work plan and (2) directs the user on how to implement ecological tasks identified in the plan. Under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA), as amended by the Superfired Amendments and Reauthorization Act of 1986 (SARA), an RI/FS work plan win have to be developed as part of the site-remediation scoping the process. Specific guidance on the RI/FS process and the preparation of work plans has been developed by the US Environmental Protection Agency (EPA 1988a). This document provides guidance to US Department of Energy (DOE) staff and contractor personnel for incorporation of ecological information into environmental remediation planning and decision making at CERCLA sites. An overview analysis of early ecological risk assessment methods (i.e., in the 1980s) at Superfund sites was conducted by the EPA (1989a). That review provided a perspective of attention given to ecological issues in some of the first RI/FS studies. By itself, that reference is of somewhat limited value; it does, however, establish a basis for comparison of past practices in ecological risk with current, more refined methods

  18. Ecological risk assessment guidance for preparation of remedial investigation/feasibility study work plans

    Pentecost, E.D.; Vinikour, W.S. [Argonne National Lab., IL (United States)

    1993-08-01

    This guidance document (1) provides instructions on preparing the components of an ecological work plan to complement the overall site remedial assessment investigation/feasibility study (RI/FS) work plan and (2) directs the user on how to implement ecological tasks identified in the plan. Under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA), as amended by the Superfired Amendments and Reauthorization Act of 1986 (SARA), an RI/FS work plan win have to be developed as part of the site-remediation scoping the process. Specific guidance on the RI/FS process and the preparation of work plans has been developed by the US Environmental Protection Agency (EPA 1988a). This document provides guidance to US Department of Energy (DOE) staff and contractor personnel for incorporation of ecological information into environmental remediation planning and decision making at CERCLA sites. An overview analysis of early ecological risk assessment methods (i.e., in the 1980s) at Superfund sites was conducted by the EPA (1989a). That review provided a perspective of attention given to ecological issues in some of the first RI/FS studies. By itself, that reference is of somewhat limited value; it does, however, establish a basis for comparison of past practices in ecological risk with current, more refined methods.

  19. Ecological risk caused by land use change in the coastal zone: a case study in the Yellow River Delta High-Efficiency Ecological Economic Zone

    China's coastal zone plays an important role in ecological services production and social-economic development; however, extensive and intensive land resource utilization and land use change have lead to high ecological risk in this area during last decade. Regional ecological risk assessment can provide fundamental knowledge and scientific basis for better understanding of the relationship between regional landscape ecosystem and human activities or climate changes, facilitating the optimization strategy of land use structure and improving the ecological risk prevention capability. In this paper, the Yellow River Delta High-Efficiency Ecological Economic Zone is selected as the study site, which is undergoing a new round of coastal zone exploitation and has endured substantial land use change in the past decade. Land use maps of 2000, 2005 and 2010 were generated based on Landsat images by visual interpretation method, and the ecological risk index was then calculated. The index was 0.3314, 0.3461 and 0.3176 in 2000, 2005 and 2010 respectively, which showed a positive transition of regional ecological risk in 2005

  20. Investigating Cooperative Behavior in Ecological Settings: An EEG Hyperscanning Study

    Petti, Manuela; He, Eric J.; De Giusti, Vittorio; He, Bin; Astolfi, Laura; Babiloni, Fabio

    2016-01-01

    The coordinated interactions between individuals are fundamental for the success of the activities in some professional categories. We reported on brain-to-brain cooperative interactions between civil pilots during a simulated flight. We demonstrated for the first time how the combination of neuroelectrical hyperscanning and intersubject connectivity could provide indicators sensitive to the humans’ degree of synchronization under a highly demanding task performed in an ecological environment. Our results showed how intersubject connectivity was able to i) characterize the degree of cooperation between pilots in different phases of the flight, and ii) to highlight the role of specific brain macro areas in cooperative behavior. During the most cooperative flight phases pilots showed, in fact, dense patterns of interbrain connectivity, mainly linking frontal and parietal brain areas. On the contrary, the amount of interbrain connections went close to zero in the non-cooperative phase. The reliability of the interbrain connectivity patterns was verified by means of a baseline condition represented by formal couples, i.e. pilots paired offline for the connectivity analysis but not simultaneously recorded during the flight. Interbrain density was, in fact, significantly higher in real couples with respect to formal couples in the cooperative flight phases. All the achieved results demonstrated how the description of brain networks at the basis of cooperation could effectively benefit from a hyperscanning approach. Interbrain connectivity was, in fact, more informative in the investigation of cooperative behavior with respect to established EEG signal processing methodologies applied at a single subject level. PMID:27124558

  1. Case study of ecological risk assessment at an Alaska airport

    An ecological risk assessment was conducted for 10 sites at a remote location that has unique biological resources. Chemicals of concern included petroleum, metals, polychlorinated biphenyls, and dioxins and furans. Risks to 23 species of mammals and birds were evaluated by using toxicity reference values and a hazard quotient approach analogous to the US Environmental Protection Agency's (USEPA's) approach for evaluating noncarcinogenic human health effects. Risks to fish and aquatic invertebrates were evaluated using risk-based concentrations for water analogous to the USEPA's water quality criteria. Risks to plants were evaluated using risk-based concentrations for soil. Toxicity reference values and risk-based concentrations were developed by applying uncertainty factors to the highest quality toxicity data available in the literature. Intake rates for wildlife were obtained from the USEPA's wildlife exposure factors handbook, or were estimated using allometric equations. The sizes of wildlife home ranges were compared with the size of each site to determine species- and site-specific exposure frequencies. Indicator chemicals were selected to represent the chemical and toxicological characteristics of petroleum fractions. The species most often at risk were found to be fish and aquatic invertebrates, as well as small-bodied, ground-dwelling or ground-feeding wildlife

  2. Ecological study of the effects of nuclear power plants on benthic macroplant microcosms in subtropical and tropical estuaries. Annual progress report, 1974--1975

    Progress is reported on the following research projects: the dynamic ecology of Thalassia and studies of the Thalassia community; basic ecology of Thalassia growth and reproduction; red macroalgal ecology; green macroalgal ecology; transplantation of Thalassia; succession in a previously damaged Thalassia community at Turkey Point; and thermal ecology of the Thalassia community of Card Sound and Turkey Point. (U.S.)

  3. RhoA Activation Sensitizes Cells to Proteotoxic Stimuli by Abrogating the HSF1-Dependent Heat Shock Response.

    Roelien A M Meijering

    Full Text Available The heat shock response (HSR is an ancient and highly conserved program of stress-induced gene expression, aimed at reestablishing protein homeostasis to preserve cellular fitness. Cells that fail to activate or maintain this protective response are hypersensitive to proteotoxic stress. The HSR is mediated by the heat shock transcription factor 1 (HSF1, which binds to conserved heat shock elements (HSE in the promoter region of heat shock genes, resulting in the expression of heat shock proteins (HSP. Recently, we observed that hyperactivation of RhoA conditions cardiomyocytes for the cardiac arrhythmia atrial fibrillation. Also, the HSR is annihilated in atrial fibrillation, and induction of HSR mitigates sensitization of cells to this disease. Therefore, we hypothesized active RhoA to suppress the HSR resulting in sensitization of cells for proteotoxic stimuli.Stimulation of RhoA activity significantly suppressed the proteotoxic stress-induced HSR in HL-1 atrial cardiomyocytes as determined with a luciferase reporter construct driven by the HSF1 regulated human HSP70 (HSPA1A promoter and HSP protein expression by Western Blot analysis. Inversely, RhoA inhibition boosted the proteotoxic stress-induced HSR. While active RhoA did not preclude HSF1 nuclear accumulation, phosphorylation, acetylation, or sumoylation, it did impair binding of HSF1 to the hsp genes promoter element HSE. Impaired binding results in suppression of HSP expression and sensitized cells to proteotoxic stress.These results reveal that active RhoA negatively regulates the HSR via attenuation of the HSF1-HSE binding and thus may play a role in sensitizing cells to proteotoxic stimuli.

  4. 苯并[a]芘对内皮细胞HSF1与HSE结合特性分析

    徐增光; 文冠华; 杨晓波; 杨进波; 邬堂春

    2005-01-01

    目的研究苯并[a]芘(BaP)作用下热休克转录因子1(HSF1)与热休克元件(HSE)结合特性,分析BaP对热应激蛋白70(HSP70)的表达的影响。方法取对数生长期的猪主动脉内皮细胞传代培养,分别以不同浓度的BaP染毒(0、0.1、0.5、1、5、10μmol/L)24h,提取细胞核蛋白,用凝胶阻滞电泳实验(EMSA)检测结合率。结果低(0.1μM)、中剂量(0.5,1μM)BaP可使HSF1-HSE结合率上升,高剂量(5,10μM)BaP使HSF1-HSE结合率明显降低。结论BaP可改变细胞HSF1-HSE结合能力,低、中剂量的BaP使HSF1-HSE结合率升高,提示可促进HSP70表达,高浓度BaP可抑制HSP70的表达。

  5. Ecological optimization and performance study of irreversible Stirling and Ericsson heat engines

    The concept of finite time thermodynamics is used to determine the ecological function of irreversible Stirling and Ericsson heat engine cycles. The ecological function is defined as the power output minus power loss (irreversibility), which is the ambient temperature times, the entropy generation rate. The ecological function is maximized with respect to cycle temperature ratio and the expressions for the corresponding power output and thermal efficiency are derived at the optimal operating conditions. The effect of different operating parameters, the effectiveness on the hot, cold and the regenerative side heat exchangers, the cycle temperature ratio, heat capacitance ratio and the internal irreversibility parameter on the maximum ecological function are studied. It is found that the effect of regenerator effectiveness is more than the hot and cold side heat exchangers and the effect of the effectiveness on cold side heat exchanger is more than the effectiveness on the hot side heat exchanger on the maximum ecological function. It is also found that the effect of internal irreversibility parameter is more than the other parameters not only on the maximum ecological function but also on the corresponding power output and the thermal efficiency

  6. Ecological Compensation Mechanism of Agricultural Water Resources: An Empirical Study in Zhejiang, China

    Qichang Hu

    2015-06-01

    Full Text Available Ecological compensation is a new area in the field of resources environment. In this study, we set an empirical analysis model about ecological compensation mechanism and analyze influential factors based on agricultural perspective. The result shows that: first, loss of development opportunities is the most important factor that will affect ecological compensation, the regression coefficient is 0.071; sec, environmental protection cost is the most significant variable in ecological compensation, the sig value is 0.007 and the regression coefficient is positive; third, public awareness has high coefficient value as 0.048, means that public awareness plays a positive effect. Overall, the regression model of the empirical results with the above assumptions, under the 10% significant level, the loss of development opportunities; environmental protection cost; the compensation standard; public awareness of the value of ecological environment variable are significantly variables that affecting ecological compensation and the effects of other variables are not obvious. On this basis, we put forward relevant suggestions.

  7. EVOLVEMENT AND CONTROL OF VULNERABLE ECOLOGICAL REGION--A Case Study in Ongniud Banner and Aohan Banner, Inner Mongolia

    RAN Sheng-hong; JIN Jian-jun

    2004-01-01

    The evolvement of a vulnerable ecological region is a dynamic process, which is affected by various factors. During the evolvement process, human activities have a decisive effect. The purpose of studying vulnerable ecological region is to control human economic activities and to develop a negative feedback modulation mechanism.This paper established a model of vulnerable ecological region's evolvement by considering four synthetic variables.These synthetic variables are ecological carrying capacity, ecological resilience, economic development intensity, and economic development velocity. Finally, Ongniud Banner and Aohan Banner in North China were taken as study cases to simulate the evolvement processes of vulnerable ecological regions under different conditions of economic development. The results show that human activities have an important influence on the evolvement trend of vulnerable ecological region.

  8. Future ecological studies of Brazilian headwater streams under global-changes

    Marcos Callisto

    2012-09-01

    Full Text Available This paper results from discussions triggered during the "Stream Ecology Symposium" that took place at the XIII Congress of the Brazilian Society of Limnology in September of 2011 in Natal, Brazil. Based on our experiences, we have raised several questions regarding ecological studies of headwater streams facing threats under global-changes and proposed numerous subjects to be addressed in future studies in Brazil. These studies deal with the necessity of knowing species biology and the elaboration of models to assess changes (which implies the availability of time-series or large-scale data sets; the ecology of riparian zones and the interchange of materials and energy across the land-water boundaries; forest conversions and standardized sampling strategies and data treatment to assess global change.

  9. Ecological forecasting under climatic data uncertainty: a case study in phenological modeling

    Forecasting ecological responses to climate change represents a challenge to the ecological community because models are often site-specific and climate data are lacking at appropriate spatial and temporal resolutions. We use a case study approach to demonstrate uncertainties in ecological predictions related to the driving climatic input data. We use observational records, derived observational datasets (e.g. interpolated observations from local weather stations and gridded data products) and output from general circulation models (GCM) in conjunction with site based phenology models to estimate the first flowering date (FFD) for three woody flowering species. Using derived observations over the modern time period, we find that cold biases and temperature trends lead to biased FFD simulations for all three species. Observational datasets resolved at the daily time step result in better FFD predictions compared to simulations using monthly resolution. Simulations using output from an ensemble of GCM and regional climate models over modern and future time periods have large intra-ensemble spreads and tend to underestimate observed FFD trends for the modern period. These results indicate that certain forcing datasets may be missing key features needed to generate accurate hindcasts at the local scale (e.g. trends, temporal resolution), and that standard modeling techniques (e.g. downscaling, ensemble mean, etc) may not necessarily improve the prediction of the ecological response. Studies attempting to simulate local ecological processes under modern and future climate forcing therefore need to quantify and propagate the climate data uncertainties in their simulations.

  10. Theories of practice - new inspiration for ecological economic studies on consumption

    Røpke, Inge

    2009-01-01

    The dynamics behind ever-increasing consumption have long been a core issue of ecological economics. Studies on this topic have traditionally drawn not only on insights from economics, but also from such disciplines as sociology, anthropology and psychology. In recent years, a practice theory app...... consumption, and the need for collective efforts supported by research into the co-evolution of domestic practices, systems of provision, supply chains and production....... relation to studies of everyday life, domestic practices and consumption, and to argue that this approach can be fruitful for ecological economics and other fields interested in the environmental aspects of consumption. The paper emphasizes the immense challenge involved in promoting sustainable...

  11. Studying stress responses in the post-genomic era: its ecological and evolutionary role

    Jesper G Sørensen; Volker Loeschcke

    2007-04-01

    Most investigations on the effects of and responses to stress exposures have been performed on a limited number of model organisms in the laboratory. Here much progress has been made in terms of identifying and describing beneficial and detrimental effects of stress, responses to stress and the mechanisms behind stress tolerance. However, to gain further understanding of which genes are involved in stress resistance and how the responses are regulated from an ecological and evolutionary perspective there is a need to combine studies on multiple levels of biological organization from DNA to phenotypes. Furthermore, we emphasize the importance of studying ecologically relevant traits and natural or semi-natural conditions to verify whether the results obtained are representative of the ecological and evolutionary processes in the field. Here, we will review what we currently know about thermal adaptation and the role of different stress responses to thermal challenges in insects, particularly Drosophila. Furthermore, we address some key questions that require future attention.

  12. Estimating survival rates in ecological studies with small unbalanced sample sizes: an alternative Bayesian point estimator

    Christian Damgaard

    2011-12-01

    Full Text Available Increasingly, the survival rates in experimental ecology are presented using odds ratios or log response ratios, but the use of ratio metrics has a problem when all the individuals have either died or survived in only one replicate. In the empirical ecological literature, the problem often has been ignored or circumvented by different, more or less ad hoc approaches. Here, it is argued that the best summary statistic for communicating ecological results of frequency data in studies with small unbalanced samples may be the mean of the posterior distribution of the survival rate. The developed approach may be particularly useful when effect size indexes, such as odds ratios, are needed to compare frequency data between treatments, sites or studies.

  13. Influence of HSF1 Gene Knockout on Mouse Growth and Reproduction%热休克因子1基因剔除对小鼠生长繁殖的影响

    陈广文; 刘喜玲; 刘可

    2009-01-01

    目的 观察HSF1基因剔除对小鼠生长、繁殖的影响.方法 用HSF1基因剔除纯合子、杂合子和野生型小鼠建立交配对,HSF1基因正常繁殖组(简称HSF1正常组)30对、HSF1基因缺陷繁殖组(简称HSF1缺陷组)72对.观察母鼠产仔数、生产胎数、每胎产仔数、成年鼠体重.结果 HSF1缺陷组母鼠平均产仔数(13.00±11.50)较少,与HSF1正常组(26.46±16.02)比较差异有显著性(P0.05).HSF1缺陷组成年小鼠平均体重(20.53±4.62)较轻,与HSF1正常组(23.06±3.39)比较差异有显著性(P<0.01).结论 HSF1基因剔除小鼠被广泛应用于研究HSF1功能,但HSF1基因剔除对生殖、生长和健康状态的影响不容忽视.

  14. Ecological studies of small vertebrates in Pu-contaminated study areas of NTS and TTR

    Ecological studies of vertebrates in plutonium-contaminated areas of the Nevada Test Site (NTS) were initiated in March 1972, and have continued to date. In September 1973, standard census methods were also employed to derive a qualitative and quantitative inventory of vertebrate biota of four Nevada Applied Ecology Group (NAEG) study areas of the Tonopah Test Range (TTR). A checklist of vertebrates of NAEG study areas of NTS and TTR is presented. Data are presented on vertebrate composition, relative abundance, and seasonal status in the study areas. Concentrations of 239Pu and 241Am were determined in pelt or skin, GI tract, and carcass of 13 lizards and 16 mammals resident on Clean Slate 2, TTR, and Area 11, NTS. A total of 71 animals were collected for radioanalysis. However, the data were not available at the time this report was written. Pu tissue burdens were highest in lizards from Area 11 GZ. Maximum values obtained in nCi/g ash were 30.9, 42.2, and 0.43 for the pelt, GI tract, and carcass, respectively. Maximum 239Pu values in tissues of small rodents from Area 11 (not from GZ) were 11.4, 6.49, and 0.20 nCi/g ash for pelt, GI tract, and carcass, respectively. Pu/Am ratios were relatively consistent in tissue samples of lizards and small mammals from Area 11 (approximately 6:1, Pu/Am). Pu/Am ratios were not consistent in vertebrates of Clean Slate 2, TTR, and appeared to be lower in carcass (28:1, Pu/Am in mammals) than GI tract (9:1, Pu/Am in mammals). Although this trend was more conspicuous in mammals, it was also evident in reptiles. (auth)

  15. Air pollution and case fatality of SARS in the People's Republic of China: an ecologic study

    Yu Shun-Zhang

    2003-11-01

    Full Text Available Abstract Background Severe acute respiratory syndrome (SARS has claimed 349 lives with 5,327 probable cases reported in mainland China since November 2002. SARS case fatality has varied across geographical areas, which might be partially explained by air pollution level. Methods Publicly accessible data on SARS morbidity and mortality were utilized in the data analysis. Air pollution was evaluated by air pollution index (API derived from the concentrations of particulate matter, sulfur dioxide, nitrogen dioxide, carbon monoxide and ground-level ozone. Ecologic analysis was conducted to explore the association and correlation between air pollution and SARS case fatality via model fitting. Partially ecologic studies were performed to assess the effects of long-term and short-term exposures on the risk of dying from SARS. Results Ecologic analysis conducted among 5 regions with 100 or more SARS cases showed that case fatality rate increased with the increment of API (case fatality = - 0.063 + 0.001 * API. Partially ecologic study based on short-term exposure demonstrated that SARS patients from regions with moderate APIs had an 84% increased risk of dying from SARS compared to those from regions with low APIs (RR = 1.84, 95% CI: 1.41–2.40. Similarly, SARS patients from regions with high APIs were twice as likely to die from SARS compared to those from regions with low APIs. (RR = 2.18, 95% CI: 1.31–3.65. Partially ecologic analysis based on long-term exposure to ambient air pollution showed the similar association. Conclusion Our studies demonstrated a positive association between air pollution and SARS case fatality in Chinese population by utilizing publicly accessible data on SARS statistics and air pollution indices. Although ecologic fallacy and uncontrolled confounding effect might have biased the results, the possibility of a detrimental effect of air pollution on the prognosis of SARS patients deserves further investigation.

  16. A holistic approach to studying social-ecological systems and its application to southern Transylvania

    Jan Hanspach

    2014-12-01

    Full Text Available Global change presents risks and opportunities for social-ecological systems worldwide. Key challenges for sustainability science are to identify plausible future changes in social-ecological systems and find ways to reach socially and environmentally desirable conditions. In this context, regional-scale studies are important, but to date, many such studies have focused on a narrow set of issues or applied a narrow set of tools. Here, we present a holistic approach to work through the complexity posed by cross-scale interactions, spatial heterogeneity, and multiple uncertainties facing regional social-ecological systems. Our approach is spatially explicit and involves assessments of social conditions and natural capital bundles, social-ecological system dynamics, and current development trends. The resulting understanding is used in combination with scenario planning to map how current development trends might be amplified or dampened in the future. We illustrate this approach via a detailed case study in southern Transylvania, Romania, one of Europe's most significant biocultural refugia. Our goal was to understand current social-ecological dynamics and assess risks and opportunities for sustainable development. Our findings show that historical events have strongly shaped current conditions and current development trends in southern Transylvania. Moreover, although external drivers (including EU policies set the general direction of regional development trajectories, local factors, including education, leadership, and the presence of bridging organizations, can enhance or counteract their effects. Our holistic approach was useful for generating an in-depth understanding of a regional social-ecological system and could be transferred to other parts of the world.

  17. ECOLOGICAL PROBLEMS OF RESTORATED TRADITIONAL SETTLEMENTS: A CASE STUDY IN KALEİÇİ (ANTALYA

    Hacer MUTLU DANACI

    2011-12-01

    show that historical urban fabric of the city is faced with the danger of losing its ecological feature.In this study, Antalya-Kaleiçi historical urban settlement which was built in the context of the ecological design criteria and which has conventional houses that achieved to reach our day has been discussed. When houses with historical fabric which are experiencing a function shift to ensure sustainability in today's conditions are analyzed in terms of ecological design criteria, we will mention the sustainability of the criteria obtained in terms of availability and the problems faced with the restoration.

  18. Incorporating ecological risk assessment into remedial investigation/feasibility study work plans

    This guidance document (1) provides instructions on preparing the components of an ecological work plan to complement the overall site remedial investigation/feasibility study (RI/FS) work plan and (2) directs the user on how to implement ecological tasks identified in the plan. Under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA), as amended by the Superfund Amendments and Reauthorization Act of 1986 (SARA), and RI/FS work plan will have to be developed as part of the site-remediation scoping process. Specific guidance on the RI/FS process and the preparation of work plans has been developed by the US Environmental Protection Agency (EPA 1988a). This document provides guidance to US Department of Energy (DOE) staff and contractor personnel for incorporation of ecological information into environmental remediation planning and decision making at CERCLA sites

  19. Study on Ecological Remediation for Water and Soil Conservation of a Small Watershed

    Chunjuan; ZHANG; Xueying; HE

    2013-01-01

    Taking the waterhead area of the middle line project for diverting water from the south to the north,Hanjiang watershed in Shiquan, as an example,ecological remediation of the small watershed was studied from aspects of necessity,practicability,plans and aims. The ecological restoration for soil and water conservation in Hanjiang River basin can not only control soil erosion and effectively protect water resources to provide clean water for people living in the lower reaches of Hanjiang mainstream,but also increase farmers’ income and protect environment, which is both typical and exemplary.

  20. Ecological shortage

    The Meadows study (Limits to Growth) has made the environmental problem popular, but it has reduced the ecological problem to one of population and raw materials, leaving the conditions of social organisation and developmental policy out of consideration. This means that in spite of the repeated moral appeals, developing countries are left to their natural fate while fear and resignation are spread in the industrial nations. The present study tries to contradict this trend in consideration of interdependences in ecological development. (orig.)

  1. HSF1抑制热应激所致RAW264.7巨噬细胞凋亡%HSF1 inhibits heat stress-induced apoptosis in Raw264.7 macrophages

    鄂顺梅; 肖卫民; 王慷慨; 王秋鹏; 刘梅冬; 刘可; 肖献忠

    2006-01-01

    目的:探讨热休克因子1(heat shock factor 1, HSF1)对热应激所致Raw264.7巨噬细胞凋亡的影响.方法:采用热应激(42.5 ℃±0.5 ℃)处理稳定表达小鼠HSF1基因的Raw264.7巨噬细胞1 h,37 ℃分别恢复6,9,12,24 h,采用流式细胞术,hoechst33258染色和DNA琼脂糖凝胶电泳检测细胞凋亡.结果:流式细胞术结果显示,热应激后对照组(转空载体)细胞凋亡核百分率较热应激前明显升高,9 h达峰值(约为60%),此时荧光染色可见30%的细胞出现核固缩,凋亡小体等典型的凋亡形态学改变;并于热应激后6,9,12 h均能检测到清晰的DNA梯状条带.与转空载体对照组相比,HSF1过表达能显著降低热应激所致凋亡及明显抑制DNA的断裂.结论:HSF1可以抑制热应激所致的Raw264.7巨噬细胞凋亡.

  2. Studies on some ecological aspects of Balanus amphitrite (Cirripedia: Thoracica)

    Desai, D.V.

    The year round breeding capability of Balanus amphitrite indicates a potential for continuous recruitment. The recruitment pattern however indicated a lull during monsoon. The study site experiences increased land run off lowering the salinity...

  3. Chironomids (Diptera, Nematocera) of Temporary Pools - an Ecological Case Study

    Dettinger-Klemm, Paul-Martin Andreas

    2004-01-01

    The main aim of the present study was to determine how Chironomidae cope with the environmental changes to which temporary pools are exposed. Are the species specifically adapted to the habitat or opportunistic? The problem was approached by: (a) an emergence study done in the Lahnberge mountain range (Marburg, Hesse, Germany) on three pools that were subjected to different lengths of drought (two of which were really temporary an...

  4. AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis

    LI Chunguang; CHEN Qijun; GAO Xinqi; QI Bishu; CHEN Naizhi; XU Shouming; CHEN Jia; WANG Xuechen

    2005-01-01

    There is increasing evidence for considerable interlinking between the responses to heat stress and oxidative stress, and recent researches suggest heat shock transcription factors (Hsfs) play an important role in linking heat shock with oxidative stress signals. In this paper, we present evidence that AtHsfA2 modulated expression of stress responsive genes and enhanced tolerance to heat and oxidative stress in Arabidopsis. Using Northern blot and quantitative RT-PCR analysis, we demonstrated that the expression of AtHsfA2 was induced by not only HS but also oxidative stress. By functional analysis of AtHsfA2 knockout mutants and AtHsfA2 overexpressing transgenic plants, we also demonstrated that the mutants displayed reduced the basal and acquired thermotolerance as well as oxidative stress tolerance but the overexpression lines displayed increased tolerance to these stress. The phenotypes correlated with the expression of some Hsps and APX1, ion leakage, H2O2 level and degree of oxidative injuries. These results showed that, by modulated expression of stress responsive genes, AtHsfA2 enhanced tolerance to heat and oxidative stress in Arabidopsis. So we suggest that AtHsfA2 plays an important role in linking heat shock with oxidative stress signals.

  5. Road ecology and Neotropical amphibians: contributions for future studies

    Paula Eveline Ribeiro D'Anunciação

    2013-12-01

    Full Text Available Many species of amphibians have suffered serious population declines. Several factors contribute separately or jointly to these declines. However, the reduction of an available habitat due to human expansion is still the main cause, and roads are a major mean for this expansion. Both the construction phase and the subsequent use of roads have negative consequences for amphibians. We reviewed the literature on the subject within the Neotropical context. To this end, the paper begins with a summary of recent reviews and proceeds through an analysis of sampling methods used in roadkill studies, mitigation measures and the Neotropical scenario and concludes with several suggestions to guide future studies. More attention will be given to roadkills, which is one of the primary impacts on wildlife that is caused by roads. Even in the Neotropical zone most studies are foot-based, the richness and abundance of amphibians affected are higher in regions outside the Neotropics. One possible explanation is that in the other regions, the proportion of studies exclusively on amphibians is bigger. Regarding mitigation measures, most studies only indicates what should be used, but do not implement or evaluate their effectiveness.

  6. Mood disorders in everyday life : A systematic review of experience sampling and ecological momentary assessment studies

    Aan het Rot, M.; Hogenelst, Koen; Schoevers, R.A.

    2012-01-01

    In the past two decades, the study of mood disorder patients using experience sampling methods (ESM) and ecological momentary assessment (EMA) has yielded important findings. In patients with major depressive disorder (MDD), the dynamics of their everyday mood have been associated with various aspec

  7. Revitalizing Traditional Ecological Knowledge: A Study in an Alpine Rural Community

    Ianni, Elena; Geneletti, Davide; Ciolli, Marco

    2015-07-01

    This study aims to contribute to the debate on the value and the role of ecological knowledge in modern conservation strategies, with reference to the results of a case study conducted in the community of Montagne, located within a World Heritage site in the Italian Alps. This community is a paradigmatic example of the multiple transformations experienced by cultural landscapes in Alpine areas under the influence of global change. This study seeks to understand whether ecological knowledge is still in place in the community, and what the relationship is between the knowledge transmission and land use and social changes that have occurred in recent decades. To that end, the community is described by identifying the key variables (social, institutional, and ecological) that have historically shaped the landscape and the future priorities of the residents. Forest expansion, the most significant change in land use in the last 60 years, is analyzed using aerial photos; changes in biodiversity-related knowledge in the community are quantified by analyzing the inter-generational differences in plant species recognition. Results are discussed in the context of the current situation of the Montagne community, and the recommendation is made that policies and actions to promote traditional ecological knowledge protection or recovery in Europe be viewed as an important part of the recovery of community sovereignty and vitality. Lastly, concrete actions that can be implemented in our case study are proposed.

  8. Ecological bases for the forestry of the natural forest, Case study of the Catival (Prioretum copaiferae)

    A first development is presented for Colombia of the ecological bases for the forestry by means of the case study of the Catival consortium (prioretum copaiferae); recapturing the results of the investigations carried out for more of 16 years for the National Corporation of Investigation and Forestall Development-CONIF and the company Pizano adding the achievements of other investigations

  9. The socio-ecological impacts of small dams: A case study of Mushandike Sanctuary, Zimbabwe

    Gwazani, R.; Gandiwa, E.; Gandiwa, P.; Mhaka, V.; Hungwe, T.; Muza, M.

    2012-01-01

    Demand and supply of water over space and time is being influenced by changes in land use, population growth, industrial development and construction of dams. In this study, we focus on Mushandike dam located in Mushandike Sanctuary, Zimbabwe, and evaluate the socio-ecological impacts associated wit

  10. Daily Emotional Dynamics in Depressed Youth: A Cell Phone Ecological Momentary Assessment Study

    Silk, Jennifer S.; Forbes, Erika E.; Whalen, Diana J.; Jakubcak, Jennifer L.; Thompson, Wesley K.; Ryan, Neal D.; Axelson, David A.; Birmaher, Boris; Dahl, Ronald E.

    2011-01-01

    This study used a new cell phone ecological momentary assessment approach to investigate daily emotional dynamics in 47 youths with major depressive disorder (MDD) and 32 no-psychopathology controls (CON) (ages 7-17 years). Information about emotional experience in the natural environment was obtained using answer-only cell phones, while MDD…

  11. College of Science graduate student awarded National Institutes of Health fellowship to study disease ecology

    Doss, Catherine

    2009-01-01

    Camille Harris of Ridgeland, Miss., a graduate student in biological sciences, has been awarded a prestigious National Institutes of Health (NIH) Graduate Research Fellowship for her study of forest disturbance and its ecological impacts on LaCrosse Virus, a mosquito-borne disease that can cause seizures, coma, paralysis, and permanent brain damage in severe cases.

  12. An Ecological Study of Gray Goose Marsh, Alviso California

    US Fish and Wildlife Service, Department of the Interior — The area of our study is located in the heart of the 300m wide strip of land just north of Triangle Marsh, known as Gray Goose Marsh. This land used to be part of...

  13. Sex specific effects of heat induced hormesis in Hsf-deficient Drosophila melanogaster

    Sørensen, J G; Kristensen, Torsten Nygård; Kristensen, K V;

    2007-01-01

    In insects mild heat stress early in life has been reported to increase life span and heat resistance later in life, a phenomenon termed hormesis. Here, we test if the induction of the heat shock response by mild heat stress is mediating hormesis in longevity and heat resistance at older age. To...... test this hypothesis we used two heat shock transcription factor (Hsf) mutant stocks. One stock harbours a mutation giving rise to a heat sensitive Hsf which inactivates the heat shock response at high temperature and the other is a rescued mutant giving rise to a wild-type phenotype. We measured...... longevity, heat resistance and expression level of a heat shock protein, Hsp70, in controls and mildly heat treated flies. We found a marked difference between males and females with males showing a beneficial effect of the early heat treatment on longevity and heat resistance later in life in the rescued...

  14. Ecological study of some parasitic helminths of aquatic organisms

    Geets, A.; P. Van Damme; Hamerlynck, O.

    1988-01-01

    Except for Monogenea, most other helminth parasites (Digenea, Cestoda, Nematoda and Acanthocephala) of aquatic organisms have a rather complex life cycle, which includes one or more intermediate hosts. Studies have been carried out on the elucidation of helminth life cycles and on parasite-host relationships. Knowledge of the feeding behaviour of the host is a very useful starting-point for elucidation of the life cycles of its' parasites. Asymphylodora demeli, a trematode of two sympatric go...

  15. Project CHOICE: #100. A Career Unit for Grades 3 and 4. Ecology Workers. (Agriculture and Ecological Studies Occupations Career Cluster).

    Kern County Superintendent of Schools, Bakersfield, CA.

    This teaching unit, Ecology Workers, is one in a series of career guides developed by Project CHOICE (Children Have Options in Career Education) to provide the classroom teacher with a source of career-related activities linking third and fourth grade elementary classroom experiences with the world of work. Part of the Agriculture and Ecological…

  16. The ecological footprint method on a farm level – a case study on a UK organic farm with parallel cropping

    Schmutz, Dr Ulrich; Firth, Chris; Lewis, Kevin; Lillywhite, Mr Robert

    2008-01-01

    There is increasing interest in the farming community to understand and improve their ecological footprint and reduce CO2-carbon emissions. This case study compares the ecological footprint of organic and conventional cabbage, celeriac, sugar beet and winter wheat crops on a UK commercial, parallel cropping, farm. Results show lower ecological footprints and energy ratios in all organic crops. However, CO2-emissions per unit yield are only lower if the fertility building is not considered. In...

  17. The importance of neighborhood ecological assets in community dwelling old people aging outcomes: A study in Northern Portugal

    Bastos, Alice M.; Faria, Carla G.; Moreira, Emília; Morais, Diana; Melo-de-Carvalho, José M.; Paul, M. Constança

    2015-01-01

    Human development is a bidirectional, person-context relational process, but scarce evidence is available about the relation between the individual variability across the life-span and the neighborhood ecological assets. Therefore, it is important that research focus not only on personal characteristics but on ecological assets as well. This way this study aims to analyze the association between neighborhood ecological assets categorized into four dimensions: human, physical or institutional,...

  18. The importance of neighborhood ecological assets in community dwelling old people aging outcomes: A study in Northern Portugal

    Carla Gomes Faria; M. Constança Paúl

    2015-01-01

    Human development is a bidirectional, person-context relational process, but scarce evidence is available about the relation between the individual variability across the life-span and the neighborhood ecological assets. Therefore, it is important that research focus not only on personal characteristics but on ecological assets as well. This way this study aims to analyze the association between neighborhood ecological assets categorized into 4 dimensions: human, physical or institutional, so...

  19. Ecological study on mangrove forest in East Coast of North Sumatra

    ONRIZAL; CECEP KUSMANA

    2008-01-01

    Ecological studies on mangrove forest in East Coast of North Sumatra have been carried out with field work in transect method and laboratory analyses. This study would be covered on floristic composition, abrasion, green belt, soil properties, and water quality of mangroves. Land system map and landsat TM imagery (year 1996 coverage) as main material in this study were used and overlay to determine training area. Based on vegetation inventory found that 20 mangrove species and by vegetation a...

  20. Ecological studies in the middle reach of Chesapeake Bay

    Passage of estuarine water and phytoplankton through the cooling system of the Calvert Cliffs nuclear power plant in the summer months of 1977-1981 resulted in frequent reductions of phytoplankton densities particularly during periods when flagellated taxa dominated total cell numbers. Entrainment-induced reductions in chlorophyll were observed on only four occasions, however. Phytoplankton productivity as oxygen evolution or 14C-uptake was even more susceptible to the effects of passing through the cooling system. Inhibition of productivitiy was observed during periods dominated by flagellated cells and generally occurred with ambient water temperature ≥ 250C. Results from track autoradiography employed in 1981 indicated that carbon fixation in the commonly observed flagellate Cryptomonas acuta was significantly depressed during July, August and September. In contrast, the dinoflagellate Prorocentrum minimum and the diatoms Cyclotella caspia and Thalassionema nitzschioides were not affected by entrainment. Although entrainment affects Cryptomonas and flagellate-dominated assemblages in the summer there are no detectable effects of power plant operations on cell densities or productivity in flagellate-dominated waters in the vicinity of the power plant. Transitory exposure of estuarine zooplankton to the elevated temperatures of CCNPP entrainment and discharge plumes was generally non-lethal. In the species selected for study the survival rate after entrainment was 65 to 100%. The effects of temperature, salinity and dissolved oxygen levels upon entrained organisms were also analyzed. (orig.)

  1. Social determinants of tuberculosis in Europe: a prospective ecological study.

    Ploubidis, George B; Palmer, Melissa J; Blackmore, Charlotte; Lim, Tek-Ang; Manissero, Davide; Sandgren, Andreas; Semenza, Jan C

    2012-10-01

    Tuberculosis (TB) is considered to be a disease of poverty, since its incidence is exacerbated by socioeconomic factors, inconsistent or partial treatment practices, and immigration from endemic countries. A prospective country level study, using a comprehensive dataset of TB incidence and prevalence taken from countries within the World Health Organization (WHO) European region, was conducted. We employed quintile regression to investigate the prospective association between baseline (measured in 2000) and a nation's wealth, level of egalitarianism, migration rate, health-related lifestyle and social capital with TB incidence and prevalence over a 10-yr period (2000-2009). We found that ∼50% of TB variation is accounted for by a nation's wealth and level of egalitarianism. We observed a negative prospective association between logged gross domestic product and TB rates, and a positive prospective association between income inequality and TB. National income levels per capita and income inequality are important predictors for TB incidence and prevalence in the WHO European region. They account for 50% of country-level variation, indicating the importance of a combined absolute and relative socioeconomic disadvantage in the development of TB. These findings also provide a tool for forecasting potential fluctuations in the level of TB epidemics in the WHO European region, with respect to socioeconomic changes. PMID:22267772

  2. The genus Crataegus L.: an ecological and molecular study.

    MirAli, N; Al-Odat, M; Haider, N; Nabulsi, I

    2011-01-01

    This study aimed at the indentification of the species and genotypes of the genus Crataegus in Syria and determination of the genetic relationships among them based on the analysis of genomic and chloroplast DNA (cpDNA) using ISSRs and CAPS techniques. Morphological characterization carried out on 49 Crataegus samples collected from different geographical regions of Syria revealed four Crataegus species: C. monogyna, C. sinaica, C. aronia and C. azarolus. In the dendrogram constructed for those samples based on ISSRs (20 primers), all samples that belong to C. monogyna were clustered in one cluster. Samples of the other three species were overlaped in another cluster. Two samples of these were the most distant from all other samples in the dendrogram and were suggested to represent hybrid species or subspecies. When CAPS technique was applied on four Crataegus samples that represent the four suggested species using 22 cpDNA regions and 90 endonucleases, no polymorphism was detected neither in amplification products sizes nor in restriction profiles. The inability of detection of variation in cpDNA among species suggested can be attributed to the low level of evolution of the cpDNA in the genus, and to the possibility that some of these species are either subspecies or hybrids since the cpDNA is inherited through one parent only. PMID:21443158

  3. The Analysis of Social Ecology and Physical Development Process of Cities (Towns Case Study: Alvand City

    Heshmati Ahmad

    2013-10-01

    Full Text Available This study aims the analysis of immigration influence on Alvand city resident's economical and living situation and its subsequent effects on Alvand city's social structure and its ecology and even its adjacency to industrial Alborz city. This analysis focuses on Alvand city's physical development using the software techniques such as GIS and using population data resulted from immigration which effects its physical development. We have increasingly witnessed the job-looking based immigrations since 1966 to 2006 which appeared differently in social, physical and spatial structure. This alternation and newly different situation has been seen apparently. The president's economic situation and immigration issue which considered as the most essential factor resulting in the ecology movements concerning Alvand city's circumstances. Such a factor could be seen in cultural varieties in Alvand city. This study also analyzes the ecological factors such as the immigration, economical situation, ecological movements and its influence on physical development using the descriptive and correlational methods to prove whether there is a logical relation among varieties providing that this statement is confirmed, this relation will be able to describe and explain the correlations in the different forms. The results show that immigration has influenced the spatial distribution of social groups and physical development of Alvand city.

  4. Comprehensive Evaluation of Urban Sprawl on Ecological Environment Using Multi-Source Data: a Case Study of Beijing

    Wang, Hao; Ning, Xiaogang; Zhu, Weiwei; Li, Fei

    2016-06-01

    With urban population growing and urban sprawling, urban ecological environment problems appear. Study on spatiotemporal characteristics of urban sprawl and its impact on ecological environment is useful for ecological civilization construction. Although a lot of work has been conducted on urban sprawl and its impact on ecological environment, resolution of images to extract urban boundary was relatively coarse and most studies only focused on certain indicators of ecological environment, rather than comprehensive evaluation of urban ecological environmental impact. In this study, high-resolution remote sensing images of Beijing from aerial photography in 2002 and 2013 respectively are employed to extract urban boundary with manual interpretation. Fractional Vegetation Coverage (FVC), Water Density (WD), Impervious Surfaces Coverage (ISC), Net Primary Production (NPP), and Land Surface Temperature (LST) are adopted to represent ecological environment. The ecological environment indicators are measured with some general algorithms by combining Landsat images, GIS data and metrological data of 243 day, 2001 and 244 day, 2013. In order to evaluate the impact of urban sprawl on ecological environment, pseudo changes due to metrological variation and other noise in this time period are removed after images calibration. The impact of urban sprawl on ecological environment is evaluated at different scales of urban extent, Beijing ring road and watershed. Results show that Beijing had been undergoing a rapid urbanization from 2002 to 2013, with urban area increase from 600 square kilometres to 987 square kilometres. All ecological environment indicators except LST became terrible in urban sprawl region, with carbon reduction of approximate 40508 tons. The Beiyun River watershed of Beijing degraded seriously since ISC increased to 0.59. Gratifyingly, ecological environment indicators including NDVI, NPP, and LST inside of 4th Ring Road became well.

  5. Ecological study on littoral and infralittoral isopods from Ubatuba, Brazil

    Ana Maria Setubal Pires

    1981-06-01

    Full Text Available The isopod species of rocky shores and shallow infralittoral zone from Enseada do Flamengo, Ubatuba, were studied. The sampling was done during Autumn (1965 and during Autumn and Springtime (1975 at six stations classified according to wave exposure. The intensity of the waves and the type of substratum showed to be important factors influencing distribution, abundance and diversity of the fauna. The highest density of isopods occurred in Dictyota ciliolata. A relation between species diversity and the degree of wave action was disclosed. Species diversity rised from exposed to moderately exposed sites and decreased with the increase in shelter.No presente trabalho, foram estudados os padrões de distribuição vertical e horizontal da fauna de Isopoda de costões rochosos, bem como sua abundância relativa nos vários substratos investigados, visando ao conhecimento de alguns aspectos de sua ecologia. As coletas foram realizadas nas zonas litoral e infralitoral da Enseada do Flamengo, Ubatuba, Estado de São Paulo, Brasil. As estações de coleta variaram quanto ao grau de exposição às ondas e profundidade. As amostras do litoral foram coletadas em abril e setembro de 1975 e janeiro de 1976, enquanto que as do infralitoral referem-se a abril e maio de 1965. Verificou-se que a exposição às ondas é um fator muito importante na distribuição das espécies, havendo animais que só foram encontrados nas condições ecológicas oferecidas por locais expostos e semi-expostos, e outros que só o foram em locais abrigados. Entretanto, existem espécies que ocorreram nas três situações, sendo geralmente mais abundantes em uma delas em particular. O tipo de substrato mostrou ser outro fator que exerce grande influência na distribuição das espécies. Substratos diferentes, coletados em um mesmo local e submetidos às mesmas condições ambientais, apresentaram variação na composição e abundância relativa da fauna de Isopoda. Esta varia

  6. Applications of C and N stable isotopes to ecological and environmental studies in seagrass ecosystems.

    Lepoint, Gilles; Dauby, Patrick; Gobert, Sylvie

    2004-12-01

    Stable isotopes of carbon and nitrogen are increasingly used in marine ecosystems, for ecological and environmental studies. Here, we examine some applications of stable isotopes as ecological integrators or tracers in seagrass ecosystem studies. We focus on both the use of natural isotope abundance as food web integrators or environmental tracers and on the use of stable isotopes as experimental tools. As ecosystem integrators, stable isotopes have helped to elucidate the general structure of trophic webs in temperate, Mediterranean and tropical seagrass ecosystems. As environmental tracers, stable isotopes have proven their utility in sewage impact measuring and mapping. However, to make such environmental studies more comprehensible, future works on understanding of basic reasons for variations of N and C stable isotopes in seagrasses should be encouraged. At least, as experimental tracers, stable isotopes allow the study of many aspects of N and C cycles at the scale of a plant or at the scale of the seagrass ecosystem. PMID:15556172

  7. Applications of C and N stable isotopes to ecological and environmental studies in seagrass ecosystems

    Lepoint, Gilles [Centre MARE, Laboratoire d' Oceanologie, Institut de Chimie, B6, Universite de Liege, B-4000 Liege (Belgium)]. E-mail: g.lepoint@ulg.ac.be; Dauby, Patrick [Centre MARE, Laboratoire d' Oceanologie, Institut de Chimie, B6, Universite de Liege, B-4000 Liege (Belgium); Institut Royal des Sciences Naturelles de Belgique, rue Vautier, B1000 Brussels (Belgium); Gobert, Sylvie [Centre MARE, Laboratoire d' Oceanologie, Institut de Chimie, B6, Universite de Liege, B-4000 Liege (Belgium)

    2004-12-01

    Stable isotopes of carbon and nitrogen are increasingly used in marine ecosystems, for ecological and environmental studies. Here, we examine some applications of stable isotopes as ecological integrators or tracers in seagrass ecosystem studies. We focus on both the use of natural isotope abundance as food web integrators or environmental tracers and on the use of stable isotopes as experimental tools. As ecosystem integrators, stable isotopes have helped to elucidate the general structure of trophic webs in temperate, Mediterranean and tropical seagrass ecosystems. As environmental tracers, stable isotopes have proven their utility in sewage impact measuring and mapping. However, to make such environmental studies more comprehensible, future works on understanding of basic reasons for variations of N and C stable isotopes in seagrasses should be encouraged. At least, as experimental tracers, stable isotopes allow the study of many aspects of N and C cycles at the scale of a plant or at the scale of the seagrass ecosystem.

  8. Studying the complexity of change: toward an analytical framework for understanding deliberate social-ecological transformations

    Michele-Lee Moore

    2014-12-01

    Full Text Available Faced with numerous seemingly intractable social and environmental challenges, many scholars and practitioners are increasingly interested in understanding how to actively engage and transform the existing systems holding such problems in place. Although a variety of analytical models have emerged in recent years, most emphasize either the social or ecological elements of such transformations rather than their coupled nature. To address this, first we have presented a definition of the core elements of a social-ecological system (SES that could potentially be altered in a transformation. Second, we drew on insights about transformation from three branches of literature focused on radical change, i.e., social movements, socio-technical transitions, and social innovation, and gave consideration to the similarities and differences with the current studies by resilience scholars. Drawing on these findings, we have proposed a framework that outlines the process and phases of transformative change in an SES. Future research will be able to utilize the framework as a tool for analyzing the alteration of social-ecological feedbacks, identifying critical barriers and leverage points and assessing the outcome of social-ecological transformations.

  9. Designing principles of an ecological water storage basin on coastal saline: a case study

    LIU Ping-ping; YIN Cheng-qing; QU Jiu-hui; ZHANG Guang-yun; FENG Wen-qing; LIU Jun-xin; ZHONG Zhi

    2005-01-01

    The degradation of water source environment becomes serious problems accompanying with rapid urbanization in China.Ecological engineering provides ecologically sound and cost-effective solution to solving this problem. As a case study, a 15 hm2 ecological water storage basin for a water plant was designed and constructed on the TEDA area in Tianjin City. Located on saline, the construction of this project has to face serious difficulties, such as high salinity, scarce seed banks of macrophytes, and strong winds. Freshwater replacement, soil emendation and macrophytes planting at the basinshore, wooden water breaker and plastic membrane installation and other measures were conducted for the assistance of plant community establishment. The result showed that the chloride concentration in the basin water decreased from 11600 mg/L to less than 100 mg/L, and the chloride content in the basin sediment decreased from 2.1% to0.35 % after freshwater soaking. The introduced macrophytes of 8 species all survived and 11 other macrophytes species were occurred in the basin. A new ecosystem was created with increased biological diversity in the original saline, and the water quality was improved. This ecological water storage basin also provided a pleasing landscape for local people.

  10. Study on the Characteristics of Ecological Water Requirement in Maijishan Scenic Spot

    Jinyong PU; Xiaowei LI

    2012-01-01

    [Objective] This study aimed to explore the characteristics of ecological water requirement in Maijishan Scenic Spot. [Methed] The characteristics of ecologi- cal water requirement in Maijishan Scenic Spot were analyzed based on,the data of soil humidity and meteorology of the spot. [Result] The result showed that the actual annual ecological water requirement in the spot was 678×10^6 m^3, and the proportion of soil water and evapotranspiration were 21% and 79%, respectively; the minimum annual ecological water requirement quota in the spot was 480.27×10^6 m^3, and the proportion of soil water and evapotranspiration were 16% and 84%, respectively; the minimum annual suitable ecological water requirement quota in the spot was 624.22×10^6 m^3, and the proportion of soil water and evapotranspiration were 18% and 82%, respectively. The precipitation was 614×10^6 m^3, and consumptive water surplus reached up to 78×10^6 m^3. The years when the precipitation was higher than the evapotranspiration accounted for 76%. Since 1980s, the evapotranspiration showed a linearly increasing trend. The precipitation was higher than the evapotranspiration from Jun. to Oct. and less than the evapotranspiration from Nov. to Dec. and Jan. to May. Evapotranspiration water requirement was regulated by soil water. The dis- parity between precipitation and evapotranspiration was huge in spring, thus having certain influence on waterfalls and streams in the spot. [Conclusion] The results of this study provided a basis for the rational use and long-term planning of the water sources in Maijishan Scenic Spot.

  11. Correlations between cutaneous malignant melanoma and other cancers: An ecological study in forty European countries

    Pablo Fernandez-Crehuet Serrano; Jose Luis Fernandez-Crehuet Serrano; Mohamed Farouk Allam; Rafael Fernandez-Crehuet Navajas

    2016-01-01

    Background: The presence of noncutaneous neoplasms does not seem to increase the risk of cutaneous malignant melanoma; however, it seems to be associated with the development of other hematological, brain, breast, uterine, and prostatic neoplasms. An ecological transversal study was conducted to study the geographic association between cutaneous malignant melanoma and 24 localizations of cancer in forty European countries. Methods: Cancer incidence rates were extracted from GLOBOCAN datab...

  12. Heterozygote Advantage Probably Maintains Rhesus Factor Blood Group Polymorphism: Ecological Regression Study

    Flegr, Jaroslav

    2016-01-01

    Rhesus factor polymorphism has been an evolutionary enigma since its discovery in 1939. Carriers of the rarer allele should be eliminated by selection against Rhesus positive children born to Rhesus negative mothers. Here I used an ecologic regression study to test the hypothesis that Rhesus factor polymorphism is stabilized by heterozygote advantage. The study was performed in 65 countries for which the frequencies of RhD phenotypes and specific disease burden data were available. I performe...

  13. Relationships between black tea consumption and key health indicators in the world: an ecological study

    Beresniak, Ariel; Duru, Gerard; Berger, Genevieve; Bremond-Gignac, Dominique

    2012-01-01

    Objectives The aim of this study was to investigate potential statistical relationships between black tea consumption and key health indicators in the world. The research question is: Does tea consumption is correlated with one or more epidemiological indicators? Design Ecological study using a systematic data-mining approach in which the unit of the analysis is a population of one country. Setting Six variables, black tea consumption data and prevalence data of respiratory diseases, infectio...

  14. Can Social Theory Adequately Address Nature-Society Issues? Do political ecology and science studies in Geography incorporate ecological change?

    Nightingale, Andrea J.

    2006-01-01

    There has been an expansion of interest in nature-society issues within human geography spurred by the rich, sophisticated analyses of environment-development issues within the Third World. This latter work emerged out of the fusion of cultural ecology and the political economy of resource use, but scholars are increasingly turning towards post-structuralism to engage with the complex, mutual constitution of symbolic and material struggles over land and resources. Yet to some e...

  15. A novel HSF4 gene mutation (p.R405X causing autosomal recessive congenital cataracts in a large consanguineous family from Pakistan

    Cheema Abdul

    2008-11-01

    Full Text Available Abstract Background Hereditary cataracts are most frequently inherited as autosomal dominant traits, but can also be inherited in an autosomal recessive or X-linked fashion. To date, 12 loci for autosomal recessive cataracts have been mapped including a locus on chromosome 16q22 containing the disease-causing gene HSF4 (Genbank accession number NM_001040667. Here, we describe a family from Pakistan with the first nonsense mutation in HSF4 thus expanding the mutational spectrum of this heat shock transcription factor gene. Methods A large consanguineous Pakistani family with autosomal recessive cataracts was collected from Quetta. Genetic linkage analysis was performed for the common known autosomal recessive cataracts loci and linkage to a locus containing HSF4 (OMIM 602438 was found. All exons and adjacent splice sites of the heat shock transcription factor 4 gene (HSF4 were sequenced. A mutation-specific restriction enzyme digest (HphI was performed for all family members and unrelated controls. Results The disease phenotype perfectly co-segregated with markers flanking the known cataract gene HSF4, whereas other autosomal recessive loci were excluded. A maximum two-point LOD score with a Zmax = 5.6 at θ = 0 was obtained for D16S421. Direct sequencing of HSF4 revealed the nucleotide exchange c.1213C > T in this family predicting an arginine to stop codon exchange (p.R405X. Conclusion We identified the first nonsense mutation (p.R405X in exon 11 of HSF4 in a large consanguineous Pakistani family with autosomal recessive cataract.

  16. [Ecological compensation based on farmers' willingness: A case study of Jingsan County in Hubei Province, China].

    Yu, Liang-liang; Cai, Yin-ying

    2015-01-01

    Farmland protection is a pressing issue in China' s major agricultural regions because of the strategic importance of these regions for national food security. This study quantified the appropriate ecological compensation criteria for farmland protection by way of estimating farmers' opportunity cost and willingness to adopt environment-friendly farming practices. Based on survey data collected from Jingsan County, Hubei Province, a Tobit model was constructed to identify factors affecting farmers' willingness to accept (WTA). The result showed that with appropriate economic compensation for farmland protection, 77.1% and 64.7% of the surveyed households were willing to reduce usage of fertilizers and pesticides. When the reduced rates of fertilizer and pesticide increased from 50%, farmers' opportunity costs of production respectively increased from 1198 and 5850 yuan to 9698 and 9750 yuan per hectare per year, and their WTA increased from 4750 and 7313 yuan to 9781 and 12393 yuan per hectare per year. Farmers' opportunity cost and WTA in reducing pesticide inputs were larger than those in reducing the same rate of fertilizer inputs, and in each case farmers' WTA was greater than their opportunity cost. A farm' s distance from township, farmers' knowledge about the ecology of farmland, and their expectation to improve the ecological environment of farmland had positive, significant effect on the farmers' WTA to reducing fertilization, while farmers' education level and the overall economic condition of the village had significantly negative effect. The proportion of agriculture income, farmers' knowledge about the ecology of farmland, and their expectation to improve the ecological environment of farmland had positive, significant effect on the farmers' WTA to reducing pesticide, while household' cash income and the overall economic condition of the village had significantly negative effect. PMID:25985673

  17. Role of green structure and ecological services: a case study of bahawalpur city, pakistan

    Cities can make broader contribution to achieve the goals of sustainable development as they are considered major consumers of resources and ecological services. Ecological services provide a range of benefits at local, regional and global levels. Terrestrial ecosystem has different components in urban environment that provides ecological services to its inhabitants. Cities not only benefit from the internal urban ecosystem but also depend upon other ecosystems beyond the city limit. Green structure is an important component in terms of making city more sustainable and habitable. Green structure in urban environment means green infrastructure that is planned and supports sustainable that is planned and supports sustainable urban development. From planning perspective, spatial structure of green space provides a basis for sustainable urban development. In sustainable perspective, green structure more than the sum of green spaces. It is considered as spatial network of open spaces, public and private gardens and parks, sports fields, allotment gardens, woodlands and recreational grounds. Therefore, it is considered as a significant part of built-up environment and major source of ecological services. To structure urban areas of sustainable development, it is necessary to develop a proportion between grey and green cities. Keeping in view, research has been conducted to investigate spatial network of green structure in planned areas of Bahawalpur City of Pakistan. This study analyzes the ecological services generated from the investigated green structures, and helped develop an approach of inter-relation between green environment and urban society. Moreover, strategies for better land-use planning in green and sustainable perspective have been proposed. (author)

  18. Site study plan for ecology, Deaf Smith County Site, Texas: Environmental Field Program: Preliminary Draft

    1987-06-01

    The Ecology Site Study Plan describes a field program consisting of studies which include surveys for endangered, threatened, and candidate species; vegetation characterization, including mapping and cover typing, plant succession, wetlands description, and preexisting stresses; and wildlife community characterization, including availability and quality of habitats and descriptions of mammal, bird, reptile, amphibian, and invertebrate populations. The plan for each study describes the need for the study, study design, data management and use, schedule and personnel requirements, and quality assurance. These studies will provide data needed to satisfy requirements contained in, or derived from, the Salt Repository Project Requirements Document (SRP-RD). 83 refs., 3 tabs.

  19. Site study plan for ecology, Deaf Smith County Site, Texas: Environmental Field Program: Preliminary Draft

    The Ecology Site Study Plan describes a field program consisting of studies which include surveys for endangered, threatened, and candidate species; vegetation characterization, including mapping and cover typing, plant succession, wetlands description, and preexisting stresses; and wildlife community characterization, including availability and quality of habitats and descriptions of mammal, bird, reptile, amphibian, and invertebrate populations. The plan for each study describes the need for the study, study design, data management and use, schedule and personnel requirements, and quality assurance. These studies will provide data needed to satisfy requirements contained in, or derived from, the Salt Repository Project Requirements Document (SRP-RD). 83 refs., 3 tabs

  20. Observations on Introduced Species Maintenance and Reproduction Characteristic of BALB/c-HSF1 Knock-out Mice%BALB/c-HSF1基因剔除小鼠的引种、保种与繁殖特性的观察

    丁志刚; 陈广文; 俞远京; 肖献忠

    2002-01-01

    对引入的BALB/c-HSF1基因剔除小鼠近一年的繁殖、生长发育情况进行了系统观察,并就三种繁殖方式的繁殖鼠交配率、受胎率、分娩率、胎间隔及哺乳期仔鼠成活率进行了比较.结果表明,与野生型(HSF1+/+)动物比较,HSF1的缺乏对动物的繁殖有一定影响;胎间隔与动物的胎次无明显的相关性,而生长曲线与BALB/c的生长曲线类似,这种现象可能与HSF1基因剔除小鼠有BALB/c的血缘有关.

  1. The Effects of Materialism and Consumer Ethics on Ecological Behavior: An Empirical Study

    Hülya Bakırtaş; G Canberk Buluşa; İbrahim Bakırtaş

    2014-01-01

    World populations have increased day by day. Thus, production volume of companies have increased depend on increasing population. Besides, fashion and/or technologyinfluence consumer’s goods or services purchasing decision. Consequently, the type and the number of product produced have increased and natural resources both more are being used and depleted. At this point has come to the fore the consumer’s environmental concern and ecological behavior and has gained importance. Studies about en...

  2. A system-wide approach to explaining variation in potentially avoidable emergency admissions: national ecological study

    O'Cathain, Alicia; Knowles, Emma; Maheswaran, Ravi; Pearson, Tim; Turner, Janette; Hirst, Enid; Goodacre, Steve; Nicholl, Jon

    2013-01-01

    Background Some emergency admissions can be avoided if acute exacerbations of health problems are managed by the range of health services providing emergency and urgent care. Aim To identify system-wide factors explaining variation in age sex adjusted admission rates for conditions rich in avoidable admissions. Design National ecological study. Setting 152 emergency and urgent care systems in England. Methods Hospital Episode Statistics data on emergency admissions were used to calculate an a...

  3. A guide to studying the socio-ecological transition in european agriculture

    González de Molina, Manuel

    2010-01-01

    This paper shows the potential of the Social Metabolism approach to study the industrialization of the agriculture. It provides information about the physical functioning of agrarian systems over time and their spatial differences. It also sheds light on how the industrialisation of agriculture occurred; in other words, how the Socio-Ecological Transition (SET) took place in agriculture. The paper begins defining the characteristic features of the Organic Agrarian Metabolism (OAM), the starti...

  4. Using Ecological Propensity Score to Adjust for Missing Confounders in Small Area Studies

    Wang, Yingbo; Richardson, Sylvia; Hansell, Anna; Blangiardo, Marta

    2016-01-01

    Small area ecological studies are commonly used in epidemiology to assess the impact of area level risk factors on health outcomes when data are only available in an aggregated form. However the estimates are often biased due to unmeasured confounders which cannot be account for. Extra confounding information can be provided through external datasets like surveys / cohorts, where the data are available at the individual level rather than at the area level; however they typically lack the geog...

  5. “Real time” genetic manipulation: a new tool for ecological field studies

    Schäfer, Martin; Brütting, Christoph; Gase, Klaus; Reichelt, Michael; Baldwin, Ian; Meldau, Stefan

    2013-01-01

    Field experiments with transgenic plants often reveal the functional significance of genetic traits important for plant performance in their natural environments. Until now, only constitutive overexpression, ectopic expression and gene silencing methods have been used to analyze gene-related phenotypes in natural habitats. These methods do not allow sufficient control over gene expression to study ecological interactions in real-time, genetic traits playing essential roles in development, or ...

  6. Chemical ecology of the carrot fly, Psila rosae (F.): laboratory and field studies

    Selby, Martin James

    2004-01-01

    The carrot fly (Psila rosae F.) is an important pest of the cultivated carrot (Daucus carota) and other crop species in the family Apiaceae, since the larvae burrow into and feed on the developing roots. Current P. rosae control relies heavily upon the use of chemical insecticides, but these are inadequate. The aims of this study were to investigate the chemical ecology of P. rosae, particularly with regard to long range attractant and repellent semiochemicals suitable for incorporation into ...

  7. Identifying Distinct Quitting Trajectories after an Unassisted Smoking Cessation Attempt: An Ecological Momentary Assessment Study

    Bachmann, Monica S.; Znoj, Hansjörg; Brodbeck, Jeannette

    2012-01-01

    Objectives: This study aimed at identifying distinct quitting trajectories over 29 days after an unassisted smoking ces- sation attempt by ecological momentary assessment (EMA). In order to validate these trajectories we tested if they predict smoking frequency up to six months later. Methods: EMA via mobile phones was used to collect real time data on smoking (yes/no) after an unassisted quit attempt over 29 days. Smoking frequency one, three and six months after the quit attempt was assesse...

  8. Association of UV radiation with Parkinson's disease incidence: A nationwide French ecologic study

    Elbaz, A; Sofiane, K; Wald, Lucien; Dugravot, A.; Singh-Manoux, A.; Moisan, F.; Kravietz, A

    2016-01-01

    International audience Meeting: 20th International Congress Abstract Number: 467 Objective: Using ultraviolet B (UV-B) as a surrogate for vitamin D levels, we conducted a nationwide ecologic study in France in order to examine the association of UV-B exposure with Parkinson's disease (PD) incidence. Background: In addition to regulating calcium homeostasis and bone metabolism, vitamin D is involved in multiple biological pathways. Lower vitamin D is associated with increased mortality, in ...

  9. STS and Political Ecology in dialogue: future directions for a social study of environmental controversies

    Cherlet, Jan

    2010-01-01

    This article argues that the two young and burgeoning academic disciplines Science and Technology Studies (sts) and Political Ecology (pe) are unusually apt for a common, interdisciplinary research programme to analyse the interrelation of environmental problems with environmental science and environmental policies. Sts and pe are disciplines that have arisen from two completely different backgrounds, but nowadays they share more than one would simply deduce from their historical development....

  10. 过表达HSF1及ASK1基因对H2O2刺激后心肌细胞内ROS水平的影响%The influences of overexpression of HSF1 and ASK1 on ROS levels in H2O2 -treated cardiac myocytes

    张磊; 梁艳艳; 牛玉宏; 姜红; 邹云增; 葛均波

    2007-01-01

    目的 研究热休克因子1(HSF1)及凋亡信号调节激酶1(ASK1)对过氧化氢(H2O2)刺激后心肌细胞内活性氧簇水平(ROS)变化的影响.方法 对不同组培养心肌细胞分别单独转染质粒HSF1,ASK1,及共转染HSF1+ASK1,48h待其充分表达后用1 mmol/LH2O2刺激心肌细胞30m in,检测细胞内ROS水平,并与相应转染后未刺激组及未转染的对照组比较,观察ROS水平的变化.结果 (1)所有H2O2刺激组心肌细胞内ROS水平均高于相同转染条件下的非刺激组(P<0.05);(2)相同H2O2刺激条件下,各组ROS水平:HSF1组低于对照组(P<0.05),ASK1组与对照组无显著差异,HSF1+ASK1组与单转HSF1组相比有升高的趋势;(3)相同H2O2刺激条件下,各组刺激后比刺激前ROS水平增高的幅度:HSF1组低于对照组(P<0.05),ASK1组与对照组无显著差别,HSF1+ASK1组与单转HSF1组相比有升高的趋势.结论 在H2O2刺激条件下,HSF1可通过降低心肌细胞内的ROS水平来发挥细胞保护作用,而ASK1对细胞内ROS水平无影响,但其可干扰HSF1对ROS的抑制作用.

  11. Case study of building of conservation coalitions to conserve ecological interactions.

    Chen, Gao; Luo, Shihong; Mei, Nianshu; Shen, Dingfang; Sun, Weibang

    2015-12-01

    We engaged experts in various fields of study (pollination ecology, chemical ecology, and ethnobotany), invited community participation, and provided environmental education in an effort to conserve an endangered birthwort (Aristolochia delavayi) and a vulnerable pipevine swallowtail (Byasa daemonius). Scientists studied the uptake and sequestration of the secondary metabolites aristolochic acids from A. delavayi leaves by different stages of pipevine swallowtail as a defense mechanism; low fruit set of the myophilous A. delavayi due to pollinator limitation; and the emission of chemical signals that attract parasitic wasps by the prepupae of B. daemonius. The results of these studies were part of an education program delivered by personnel of non-governmental organizations. The program was devised to deliver information to the public about the health risks of consuming A. delavayi individuals (aristolochic-acid-associated cancers) and to establish a bridge between the public and scientific research. Following delivery of the program, the behavior of residents changed considerably. Community residents were involved in management activities, including participation in a program to promote understanding of ecological interactions between A. delavayi and B. daemonius; designing an in situ conservation site; monitoring A. delavayi and B. daemonius individuals; and promoting the natural fruit set of A. delavayi by scattering animal excrement to attract fly pollinators. The integration of scientific information and community participation appears to have resulted in an increase in abundance of threatened A. delavayi and B. daemonius populations. We believe the involvement of local people in conservation is necessary for successful species conservation. PMID:26372410

  12. In vitro research on the molecular mechanism of the functions of HSF1, HMGB1 and IL-10%HSF1与HMGB1和IL-10作用分子机制的体外研究及意义

    艾云智; 陈丹; 罗文福; 张先安; 罗成群

    2012-01-01

    目的:本研究以RAW264.7细胞系为研究对象,烧伤血清刺激建立烧伤细胞模型,在体外研究HSF1的抗炎症机制.方法:Western blot法检测烧伤血清刺激前后RAW264.7细胞中HSF1与NF-κB表达,凝胶阻滞法(EMSA)检测HSF1与炎症因子HMGB1和抗炎症因子IL-10基因启动子区相互作用,同时检测NF-κB是否参与上述的相互作用.结果:正常培养条件下的RAW264.7细胞几乎不表达HSF1,少量表达NF-κB,加入烧伤血清刺激后大量表达HSF1和NF-κB.HSF1与HMGB1和IL-10基因的启动子区HSE有相互作用.NF-κB不参与HSF1与HMGB1和IL-10基因启动子区HSE的结合.结论:HSF1与HMGB1和IL-10的启动子区HSE结合,抑制HMGB1的表达,促进IL-10的表达.在炎症反应中HMGB1和IL-10的表达不直接受NF-κB的调节.

  13. Ecological stability of landscape - ecological infrastructure - ecological management

    The Field Workshop 'Ecological Stability of Landscape - Ecological Infrastructure - Ecological Management' was held within a State Environmental Programme financed by the Federal Committee for the Environment. The objectives of the workshop were to present Czech and Slovak approaches to the ecological stability of the landscape by means of examples of some case studies in the field, and to exchange ideas, theoretical knowledge and practical experience on implementing the concept of ecological infrastructure in landscape management. Out of 19 papers contained in the proceedings, 3 items were inputted to the INIS system. (Z.S.)

  14. Comprehensive evaluation of regional ecological security for land use: a case study of Yanchi County, Ningxia Province

    2008-01-01

    As an Abstract study conception,most researches of ecological security generally are macro-scale theoretical study with a regional,national or global perspective.Micro-scale research mainly concentrates on ecological risk,ecosystem health and ecosystem safety.In order to assess regional ecological security and to accomplish the transition from micro-scale to macro-scale research,a Driving-Function-State-Output-Respond model framework (DFSOR model) is put forward in this paper according to the analyses on regional population,society,economy,resources,environment and ecological risks.In empirical research an index .system is set out to evaluate ecological security of land use.The DFSOR model comprises five group of indicators:(1) driving indicators,which include social population pressure and economic pressure;(2) Junction indicators,which include human function,hydrodynamic function,wind function and gravity function;(3) state indicators,which include soil resources,water resources and land use/land cover;(4) output indicators,which include production output and ecological risk output;(5) response indicators,which are composed of all sorts of policies and measures for improving production of ecosystem.The method and procedure for ecological security evaluation is put forward based on DFSOR model as well.In the case study,Yanchi,the ecotone between agriculture and animal husbandry,is chosen for the study.An index system built to evaluate ecological security based on the evaluation of regional ecological risks which including sandification,water erosion,soil salinization,soil pollution and shortage of water.The comprehens'ive evaluation result shows the regional ecological security index of land use in the research area increase obviously.But it is still in the state of low-graded danger.

  15. Thermal ecology of montane Atelopus (Anura: Bufonidae): A study of intrageneric diversity.

    Rueda Solano, Luis Alberto; Navas, Carlos A; Carvajalino-Fernández, Juan Manuel; Amézquita, Adolfo

    2016-05-01

    Harlequin frogs (Bufonidae: Atelopus) are among the most threatened frog genus in the world and reach very high elevations in the tropical Andes and the Sierra Nevada de Santa Marta (SNSM). Learning about their thermal ecology is essential to infer sensitivity to environmental changes, particularly climate warming. We report on the activity temperature and thermoregulatory behavior of three high-elevation species of harlequin frogs, Atelopus nahumae, Atelopus laetissimus and Atelopus carrikeri. The first two mentioned live in streams in Andean rain forests, whereas A. carrikeri inhabits paramo streams in the SNSM. We studied the thermal ecology of these species in tree localities differing in altitude, and focused on activity body, operative, substrate and air temperature. A main trend was lower body temperature as elevation increased, so that differences among species were largely explained by differences in substrate temperature. However, this temperature variation was much lower in forest species than paramo species. The Atelopus species included in this work proved to be thermoconformers, a trend that not extended to all congenerics at high elevation. This diversity in thermal ecology poses important questions when discussing the impact of climate warming for high-elevation harlequin frogs. For example, forest species show narrow thermal ranges and, if highly specialized, may be more susceptible to temperature change. Paramo species such as A. carrikeri, in contrast, may be more resilient to temperature change. PMID:27157338

  16. 过表达热休克因子1突变体对RAW264.7细胞的影响%The effects of HSF1 mutant over-expression on RAW264.7 cells

    梁秋娟; 张华莉; 张玲俐; 涂自智

    2009-01-01

    目的 建立稳定转染热休克因子1(HSF1)显性正性和显性负性突变体的细胞株,并探讨HSF1突变体过表达对细胞生长的影响.方法 用脂质体将真核表达质粒pcDNA3.1(+)-HSF1+和pcDNA3.1(+)-HSF1-分别转染RAW264.7细胞株,G418筛选阳性单克隆,Western blot鉴定高表达的克隆,流式细胞术检测稳定转染HSF1突变体的细胞与转空载体细胞的生长及凋亡情况.结果 建立了稳定表达HSF1显性正性或显性负性突变体的细胞株,并发现转染HSF1突变体细胞能影响正常细胞增殖,但不引起细胞凋亡.结论 HSF1突变体能影响RAW264.7细胞正常生长周期.

  17. ECOLOGICAL EDUCATION

    GABRIELA GYONGY MIHUT

    2011-04-01

    Full Text Available While in most emerging and developing countries, the population has a lower ecological footprint in the developed countries have a larger footprint.There is also an alarming contrast between a person perception of her liability for damages to its environment and its actual size. These misconceptions may have their source in the absence of awareness of risks from climate change, culture or religion.The purpose of this study is to analyze the situation at the international and Romanian level and to draw attention on the necessity of un ecological education.

  18. Immunology in wild nonmodel rodents: an ecological context for studies of health and disease.

    Jackson, J A

    2015-05-01

    Transcriptomic methods are set to revolutionize the study of the immune system in naturally occurring nonmodel organisms. With this in mind, the present article focuses on ways in which the use of 'nonmodel' rodents (not the familiar laboratory species) can advance studies into the classical, but ever relevant, epidemiologic triad of immune defence, infectious disease and environment. For example, naturally occurring rodents are an interesting system in which to study the environmental stimuli that drive the development and homeostasis of the immune system and, by extension, to identify where these stimuli are altered in anthropogenic environments leading to the formation of immunopathological phenotypes. Measurement of immune expression may help define individual heterogeneity in infectious disease susceptibility and transmission and facilitate our understanding of infection dynamics and risk in the natural environment; furthermore, it may provide a means of surveillance that can filter individuals carrying previously unknown acute infections of potential ecological or zoonotic importance. Finally, the study of immunology in wild animals may reveal interactions within the immune system and between immunity and other organismal traits that are not observable under restricted laboratory conditions. Potentiating much of this is the possibility of combining gene expression profiles with analytical tools derived from ecology and systems biology to reverse engineer interaction networks between immune responses, other organismal traits and the environment (including symbiont exposures), revealing regulatory architecture. Such holistic studies promise to link ecology, epidemiology and immunology in natural systems in a unified approach that can illuminate important problems relevant to human health and animal welfare and production. PMID:25689683

  19. AFSC/NMML: Bowhead Whale Feeding Ecology Study (BOWFEST): Aerial Survey in Chukchi and Beaufort Seas, 2007-2011

    National Oceanic and Atmospheric Administration, Department of Commerce — The Bowhead Whale Feeding Ecology Study (BOWFEST) was initiated in May 2007 through an Interagency Agreement between the Bureau of Ocean Energy Management (BOEM)...

  20. Progress Report: Integrated Ecological Studies at Lisbon Bottom Unit, Big Muddy Fish and Wildlife Refuge, Fiscal Year 1999

    US Fish and Wildlife Service, Department of the Interior — The U.S. Geological Survey has been carrying out integrated ecological studies at the Lisbon Bottom Unit of the Big Muddy Fish and Wildlife Refuge since 1996. This...

  1. Studies of the breeding and population ecology of seabirds at Semidi Islands, Alaska: A summary of work completed in 1978

    US Fish and Wildlife Service, Department of the Interior — Species mentioned include Fulmar, petrel, gull, kittiwake, murre, auklet, and puffin. Continued study of the reproductive ecology of Northern Fulmar.

  2. Global approach to ecological qualification of industrial products: Leather industry case-study; La qualificazione ecologica dei prodotti industriali: il case-study della produzione conciaria

    Grasso, G. [Ministero degli Affari Esteri, Rome (Italy); Bufalo, G. [Potenza Univ. (Italy). Facolta di Agraria; Manzo, G. [Salerno Univ. (Italy). Facolta di Economia e Commercio

    1994-09-01

    This paper deals with both economic and technical points of view of the arising question of ecological normalization of industrial products, for instance via informative quality marks. This is accomplished introducing adequate definitions of terms and specifications of objectives, based on such ecological criteria as global life-cycle of the product (`from cradle to grave`) and energy and matter complete process balance. In order to explain a concrete analysis of similar labelling and computing procedures, the specific case-study of environmental impact of leather goods was faced up. Regulation and deregulation measures of public policy concerning the environmental questions are also discussed, some emphasis doing on the normalization tools as ecolabel and ecoaudits. Finally economic analysis of ecological products is performed, involving concepts as ecological value, net added value, utilization value and ecological premium-price. Environmental factory costs for pollution control are so firstly changed in ecological quality characteristics and next in competitive market opportunities.

  3. An Ecological Flood Control System in Phoenix Island of Huzhou, China: A Case Study

    Zhuowen Wang; Jun Liu; Cheng Gao

    2013-01-01

    Traditional flood control systems always have a conflict with natural ones, i.e., rivers in cities are usually straight and smooth, whereas natural ones are according to ecological mechanisms. Social and economic developments in the modern world require a new system combining ecological needs and traditional flood control system. Ecological flood control systems were put forward and defined as flood control systems with full consideration of ecological demands for sustainable development. In ...

  4. ECOLOGICAL STUDIES ON ZOOPLANKTON OF THE WEST LAKE AND THE INFLOWS

    Li, Gouguo; Wei, Chongde; Zhou, Hong; Pei, Hongping

    1999-01-01

    Zooplankton ecology of the West Lake and the streams that flow into the lake were investigated during January-December 1995. Protozoans were the predominant group among 252 species of zooplankton, accounting for 50.4% of the total number. Due to the seasonal variations in density and biomass of zooplankton, were studied. Changqiao stream recorded the highest density of zooplankton among seven sampling points, with an average of 10,710 inds./L. The lowest density found was Jinsha stream (886 i...

  5. Study on City Environment, Safety and Health from the Viewpoint of Ecological Risks

    YIN Qian-liang

    2011-01-01

    Urban ecological risk analysis is a relatively new study field.Rapid industrial moderni ation and urbanization have significantly improved the living standards of the city.However, as environmental, safety and health issues are causing widespread concern, these problems have potential serious threat on ecosystems and human health.So how to solve many problems arising from city has become a key to sustainable development of human civilization.The present article analyzed the major problems that the city confronts and pointed out the main measures from the aspects of urban environment, safety and health.

  6. Students' exposure and career aspirations in ecology: A study using semi-structured interviews to gain knowledge of public school students

    Miller, Heather C.

    Ecology as a field is dominated by white males, McCarter (2003) has noted that women and minorities are underrepresented in the discipline of ecology across the United States. The contribution of this research is to assess and quantify, in a scientific manner, students' exposure, and career aspirations towards ecology; 226 student responses were coded from semi-structured interviews. The main objectives of this study, using student interviews, were the following: (1) assess the importance of exposure to ecology and ecological related topics to: gender, ethnicity, region, grades in science, grades in non-science, grade level, and interest in ecology career. (2) determine if early exposure to ecology (i.e. gained in high school) and ecological related topics is related to an increased interest of students continuing in an ecologically related field and (3) assess if high school students who have been involved in more outdoor related activities such as camping, hiking, hunting, and/or fishing, will be more likely to be interested in an ecological career. Overall, the results indicated that students interviewed for this study generally responded in a positive manner, and were generally interested in ecology. Some students were even interested in pursuing a career in ecology. The study revealed significant differences in the exposure of ecology between school locations, girls and boys, and whites and non-whites. The results of this research and avenues for future research are discussed.

  7. Converting redox signaling to apoptotic activities by stress-responsive regulators HSF1 and NRF2 in fenretinide treated cancer cells.

    Kankan Wang

    Full Text Available BACKGROUND: Pharmacological intervention of redox balance in cancer cells often results in oxidative stress-mediated apoptosis, attracting much attention for the development of a new generation of targeted therapy in cancer. However, little is known about mechanisms underlying the conversion from oxidative signaling to downstream activities leading cells to death. METHODOLOGY/PRINCIPAL FINDINGS: We here report a systematic detection of transcriptome changes in response to oxidative signals generated in leukemia cells upon fenretinide treatment, implicating the occurrence of numerous stress-responsive events during the fenretinide induced apoptosis, such as redox response, endoplasmic reticulum stress/unfolded protein response, translational repression and proteasome activation. Moreover, the configuration of these relevant events is primarily orchestrated by stress responsive transcription factors, as typically highlighted by NF-E2-related factor-2 (NRF2 and heat shock factor 1 (HSF1. Several lines of evidence suggest that the coordinated regulation of these transcription factors and thus their downstream genes are involved in converting oxidative signaling into downstream stress-responsive events regulating pro-apoptotic and apoptotic activities at the temporal and spatial levels, typifying oxidative stress-mediated programmed death rather than survival in cancer cells. CONCLUSIONS/SIGNIFICANCE: This study provides a roadmap for understanding oxidative stress-mediated apoptosis in cancer cells, which may be further developed into more sophisticated therapeutic protocols, as implicated by synergistic induction of cell apoptosis using proteasome inhibitors with fenretinide.

  8. Bioremediation in marine ecosystems: a computational study combining ecological modelling and flux balance analysis

    Marianna eTaffi

    2014-09-01

    Full Text Available The pressure to search effective bioremediation methodologies for contaminated ecosystems has led to the large-scale identification of microbial species and metabolic degradation pathways. However, minor attention has been paid to the study of bioremediation in marine food webs and to the definition of integrated strategies for reducing bioaccumulation in species. We propose a novel computational framework for analysing the multiscale effects of bioremediation at the ecosystem level, based on coupling food web bioaccumulation models and metabolic models of degrading bacteria. The combination of techniques from synthetic biology and ecological network analysis allows the specification of arbitrary scenarios of contaminant removal and the evaluation of strategies based on natural or synthetic microbial strains.In this study, we derive a bioaccumulation model of polychlorinated biphenyls (PCBs in the Adriatic food web, and we extend a metabolic reconstruction of Pseudomonas putida KT2440 (iJN746 with the aerobic pathway of PCBs degradation. We assess the effectiveness of different bioremediation scenarios in reducing PCBs concentration in species and we study indices of species centrality to measure their importance in the contaminant diffusion via feeding links.The analysis of the Adriatic sea case study suggests that our framework could represent a practical tool in the design of effective remediation strategies, providing at the same time insights into the ecological role of microbial communities within food webs.

  9. Ecological agriculture in South-India : an agro-economic comparison and study of transition

    Jager, de, A.E.J.; Werf, van der, F.

    1992-01-01

    This report describes two research programmes carried out on ecological agriculture in South-India. Experiences of twelve farmers in transition towards ecological agriculture are described and analysed. The comparative performance of seven farmer pairs, consisting of one ecological and one conventional reference farm, is analysed in relation to agronomic and economic performance

  10. Simulation of Regionally Ecological Land Based on a Cellular Automation Model: A Case Study of Beijing, China

    Xiubin Li

    2012-08-01

    Full Text Available Ecological land is like the “liver” of a city and is very useful to public health. Ecological land change is a spatially dynamic non-linear process under the interaction between natural and anthropogenic factors at different scales. In this study, by setting up natural development scenario, object orientation scenario and ecosystem priority scenario, a Cellular Automation (CA model has been established to simulate the evolution pattern of ecological land in Beijing in the year 2020. Under the natural development scenario, most of ecological land will be replaced by construction land and crop land. But under the scenarios of object orientation and ecosystem priority, the ecological land area will increase, especially under the scenario of ecosystem priority. When considering the factors such as total area of ecological land, loss of key ecological land and spatial patterns of land use, the scenarios from priority to inferiority are ecosystem priority, object orientation and natural development, so future land management policies in Beijing should be focused on conversion of cropland to forest, wetland protection and prohibition of exploitation of natural protection zones, water source areas and forest parks to maintain the safety of the regional ecosystem.