ecological genomics steps: Topics by WorldWideScience.org

Involvement of the Matrix and Nucleocapsid Domains of the Bovine Leukemia Virus Gag Polyprotein Precursor in Viral RNA Packaging

BackgroundThe order Tetraodontiformes consists of approximately 429 species of fishes in nine families. Members of the order exhibit striking morphological diversity and radiated...Full Text Available

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Application of Landscape Ecology to Ecological Restoration

The RNA packaging process for retroviruses involves a recognition event of the genome-length viral RNA by the viral Gag polyprotein precursor (PrGag), an important step in particle morphogenesis. The...Full Text Available

2003-09-01

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Genomic analysis of the symbiotic marine crenarchaeon, Cenarchaeumsymbiosum

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2004-10-01

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Toward Establishing a Spratly Islands International Marine Peace Park: Ecological Importance and Supportive Collaborative Activities with an Emphasis on the Role of Taiwan

Crenarchaea are ubiquitous and abundant microbial constituents of soils, sediments, lakes and ocean waters, yet relatively little is known about their fundamental evolutionary, ecological, and physiological properties. To better describe the ubiquitous nonthermophilic Crenarchaea, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont, Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although synthetic, overlapping a- and b-type ribotypes harbored significant genetic variability. A single tiling path comprising the dominant a-type genotype was assembled, and used to explore the biological properties of C. symbiosum and its planktonic relatives. Out of a total of 2,066 predicted open reading frames, 36% were more highly conserved with other Archaea. The remainder ...

2006-06-24

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British Library Electronic Table of Contents (United Kingdom)

The Spratly Islands constitute one of the earth's most ecologically significant areas, hosting a high diversity of marine species, providing critical habitats for endangered species, and providing marine larvae to reestablish depleted stocks among the heavily overfished and degraded coastal ecosystems of the South China Sea. Territorial disputes have led to the establishment of environmentally destructive, socially and economically costly military outposts on many of the islands. Given the rapid proliferation of international peace parks around the world, it is time to take positive steps toward the establishment of a Spratly Islands Marine Peace Park. Its purpose would be to manage the area's natural resources and alleviate regional tensions via a freeze on claims and claim supportive act...

2010-01-01

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Ecologically Safe Luminescent Lamps

Genome Sequence of a Lancefield Group C Streptococcus zooepidemicus Strain Causing Epidemic Nephritis: New Information about an Old Disease

Development and Creation of Model Samples of Ecologically Safe Luminescent Lamps

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Fiscal 1998 industrial science and technology R and D project. Research report on R and D of genome informatics technology (Development of stable oil supply measures using complex biosystem); 1998 nendo genome informatics gijutsu kenkyu kaihtsu seika hokokusho. Fukugo seibutsukei riyo sekiyu antei kyokyu taisaku kaihatsu

Outbreaks of disease attributable to human error or natural causes can provide unique opportunities to gain new information about host-pathogen interactions and new leads for pathogenesis research. Poststreptococcal glomerulonephritis (PSGN), a sequela of infection with pathogenic streptococci, is a common cause of preventable kidney disease worldwide. Although PSGN usually occurs after infection with group A streptococci, organisms of Lancefield group C and G also can be responsible. Despite decades of study, the molecular pathogenesis of PSGN is poorly understood. As a first step toward gaining new information about PSGN pathogenesis, we sequenced the genome of Streptococcus equi subsp. zooepidemicus strain MGCS10565, a group C organism that caused a very large and unusually severe epidemic of nephritis in Brazil. The genome is a circular chromosome of 2,024,171 bp. The genome shares extensive gene ...

2008-08-21

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Life Under Your Feet: An End-to-End Soil Ecology Sensor Network, Database, Web Server, and Analysis Service

This report describes the fiscal 1998 result on development of genome informatics technology. As comparative analysis technique of genes, the combination of electrophoresis and PCR was used. For improvement of the throughput and reproducibility of the technique, module- shuffling primers were used, and the multi(96)-arrayed capillary fragment analyzer was devised. The system detecting SNPs rapidly was also developed successfully. As analysis technology of DNA sequence by use of triple- stranded DNA formation, study was made on construction of long cDNA libraries, selective subtraction of specific sequences from libraries, and the basic technology of homologous cloning. Study was also made on each reaction step of IGCR technique for fast analysis, and specifications of a fluorescence transfer monitor. As modeling technique of genetic sequence information, the simulation model was developed for gene expression regulatory networks during muscle ...

1999-03-01

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Landscape Ecological Changes of Large Dams and Reservoir in China

Wireless sensor networks can revolutionize soil ecology by providing measurements at temporal and spatial granularities previously impossible. This paper presents a soil monitoring system we developed and deployed at an urban forest in Baltimore as a first step towards realizing this vision. Motes in this network measure and save soil moisture and temperature in situ every minute. Raw measurements are periodically retrieved by a sensor gateway and stored in a central database where calibrated versions are derived and stored. The measurement database is published through Web Services interfaces. In addition, analysis tools let scientists analyze current and historical data and help manage the sensor network. The article describes the system design, what we learned from the deployment, and initial results obtained from the sensors. The system measures soil factors with unprecedented temporal precision. However, the deployment required ...

2007-01-01

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Development of the National Ecological Observatory Network (NEON)

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2004-02-01

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Applications of natural carbon isotope variations to nutrition and ecology

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2007-02-01

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Root Hairs Play a Key Role in the Endophytic Colonization of Olive Roots by Pseudomonas spp. with Biocontrol Activity

Abstract only.

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British Library Electronic Table of Contents (United Kingdom)

The use of indigenous bacterial root endophytes with biocontrol activity against soil-borne phytopathogens is an environmentally-friendly and ecologically-efficient action within an integrated disease management framework. The earliest steps of olive root colonization by Pseudomonas fluorescens PICF7 and Pseudomonas putida PICP2, effective biocontrol agents (BCAs) against Verticillium wilt of olive (Olea europaea L.) caused by the fungus Verticillium dahliae Kleb., are here described. A gnotobiotic study system using in vitro propagated olive plants, differential fluorescent-protein tagging of bacteria, and confocal laser scanning microscopy analysis have been successfully used to examine olive roots?Pseudomonas spp. interactions at the single-cell level. In vivo simultaneous visualization...

2011-01-01

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Hydrological Classification of Natural Flow Regimes to Support Environmental Flow Assessments in Intensively Regulated Mediterranean Rivers, Segura River Basin (Spain)

British Library Electronic Table of Contents (United Kingdom)

Hydrological classification constitutes the first step of a new holistic framework for developing regional environmental flow criteria: the ?Ecological Limits of Hydrologic Alteration (ELOHA)?. The aim of this study was to develop a classification for 390 stream sections of the Segura River Basin based on 73 hydrological indices that characterize their natural flow regimes. The hydrological indices were calculated with 25?years of natural monthly flows (1980/81?2005/06) derived from a rainfall-runoff model developed by the Spanish Ministry of Environment and Public Works. These indices included, at a monthly or annual basis, measures of duration of droughts and central tendency and dispersion of flow magnitude (average, low and high flow conditions). Principal Component Analysis (PCA) indi...

2011-01-01

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Production of Infectious Genotype 1b Virus Particles in Cell Culture and Impairment by Replication Enhancing Mutations

Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured human hepatoma cells, but save for the genotype 2a isolate JFH-1, efficient replication of these HCV RNAs requires replication enhancing mutations (REMs), previously also called cell culture adaptive mutations. These mutations cluster primarily in the central region of non-structural protein 5A (NS5A), but may also reside in the NS3 helicase domain or at a distinct position in NS4B. Most efficient replication has been achieved by combining REMs residing in NS3 with distinct REMs located in NS4B or NS5A. However, in spite of efficient replication of HCV genomes containing such mutations, they do not support production of infectious virus particles. By using the genotype 1b isolate Con1, in this study we show that REMs interfere with HCV ...

2009-06-12

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Structural and operational complexity of the Geobacter sulfurreducens genome

The Drosophila hairy gene encodes a basic helix- loop-helix protein that functions in at least two steps during Drosophila development: (1) during embryogenesis, when it partakes in the establishment of segments, and (2) during the larval stage, when it functions negatively in determining the pattern of sensory bristles on the adult fly. In the rat, a structurally homologous gene (RHL) behaves as an immediate-early gene in its response to growth factors and can, like that in Drosophila, suppress neuronal differentiation events. Here, the authors report the genomic cloning of the human hairy gene homolog (HRY). The coding region of the gene is contained within four exons. The predicted amino acid sequence reveals only four amino acid differences between the human and rat genes. Analysis of the DNA sequence 5[prime] to the coding region reveals a putatitve untranslated exon. To increase the value of the HRY gene as a genetic marker and to assess ...

1994-03-01

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Intrapopulation Genome Size Dynamics in Festuca pallens

Prokaryotic genomes can be annotated based on their structural, operational, and functional properties. These annotations provide the pivotal scaffold for understanding cellular functions on a genome-scale,...Full Text Available

2010-09-01

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Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta

Background and AimsIt is well known that genome size differs among species. However, information on the variation and dynamics of genome size in wild populations and on the early...Full Text Available

2008-10-01

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British Library Electronic Table of Contents (United Kingdom)

Abstract Plant metabolomics has been well established and applied across multiple fields including medicine, biotechnology, and environmental sciences in the post-genomic era. The Chenopodiaceae C3 halophyte Suaeda salsa is the most important plant species in the vegetation of saline soil and even intertidal zone in the Yellow River Delta, which is economically consumed as food, widely used as a bioindicator of environmental stresses (salinity, drought, and pollution) and typically applied for the phyto-remediation of degraded wetland. However, no global studies have been focused on the metabolic profile of this halophyte which is widely applied in environment related research areas. In metabolomics, the first crucial step is the preparation of plant samples. In this work, several strategi...

2011-01-01

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Genomic survey of prepulse inhibition in mouse chromosome substitution strains

British Library Electronic Table of Contents (United Kingdom)

Prepulse inhibition (PPI) is a measure of sensorimotor gating, a pre-attentional inhibitory brain mechanism that filters extraneous stimuli. Prepulse inhibition is correlated with measures of cognition and executive functioning, and is considered an endophenotype of schizophrenia and other psychiatric illnesses in which patients show PPI impairments. As a first step toward identifying genes that regulate PPI, we performed a quantitative trait locus (QTL) screen of PPI phenotypes in a panel of mouse chromosome substitution strains (CSSs). We identified five CSSs with altered PPI compared with the host C57BL/6J strain: CSS-4 exhibited decreased PPI, whereas CSS-10, -11, -16 and -Y exhibited higher PPI compared with C57BL/6J. These data indicate that A/J chromosomes 4, 10, 11, 16 and Y harbor...

2009-01-01

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DNA repair: As influenced by age, nutrition, and exposure to toxic substances

Whole-genome shotgun assembly and comparison of human genome assemblies

In evaluating the risk associated with low levels of exposure to toxicants, it is clear that DNA repair, one of the main defenses against agent damage, is not a constant. It can be modified by age, time of day, and physiological state. Nutrition, especially caloric restriction (CR), can modify almost every step in the process of protecting genomic integrity. And history of exposure can modify DNA repair. Thus, the conditions of exposure are almost as important to toxicity as the exposure itself, even at the level of DNA repair. Extrapolation from high to low dose, to be consistent with what is known, should be less a mathematical exercise than an exercise in toxicological judgement, which puts the exposure in proper perspective. This appears to be true at almost every level in the process including a response with a toxic stimulus, even those thought to be very basic, such as DNA repair.

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The complete mitochondrial genome of the stomatopod crustacean Squilla mantis.

We report a whole-genome shotgun assembly (called WGSA) of the human genome generated at Celera in 2001. The Celera-generated shotgun data set consisted of 27 million sequencing reads organized in pairs...Full Text Available

2004-02-17

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The complete mitochondrial genome of the stomatopod crustacean Squilla mantis

PMID:16091132

2005-08-09

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Molecular Imaging in the Age of Genomic Medicine

BackgroundAnimal mitochondrial genomes are physically separate from the much larger nuclear genomes and have proven useful both for phylogenetic studies and for understanding genome...Full Text Available

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Genomics - from Neanderthals to high-throughput sequencing

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2007-06-01

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Systems Biology Approach in Chlamydomonas Reveals Connections between Copper Nutrition and Multiple Metabolic Steps[C][W][OA

A report on 'The Biology of Genomes' meeting, Cold Spring Harbor, USA, 10-14 May 2006.

2006-01-01

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A microscale protein NMR sample screening pipeline

In this work, we query the Chlamydomonas reinhardtii copper regulon at a whole-genome level. Our RNA-Seq data simulation and analysis pipeline validated a 2-fold cutoff and 10 RPKM (reads per kilobase of mappable length per million mapped reads) (~1 mRNA per cell) to reveal 63 CRR1 targets plus another 86 copper-responsive genes. Proteomic and immunoblot analyses captured 25% of the corresponding proteins, whose abundance was also dependent on copper nutrition, validating transcriptional regulation as a major control mechanism for copper signaling in Chlamydomonas. The impact of copper deficiency on the expression of several O2-dependent enzymes included steps in lipid modification pathways. Quantitative lipid profiles indicated increased polyunsaturation of fatty acids on thylakoid membrane digalactosyldiglycerides, indicating a global impact of copper deficiency on the photosynthetic apparatus. Discovery of a putative plastid copper chaperone ...

2011-04-01

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The role of gene expression in ecological speciation

As part of efforts to develop improved methods for NMR protein sample preparation and structure determination, the Northeast Structural Genomics Consortium (NESG) has implemented an NMR screening pipeline for protein target selection, construct optimization, and buffer optimization, incorporating efficient microscale NMR screening of proteins using a micro-cryoprobe. The process is feasible because the newest generation probe requires only small amounts of protein, typically 30-200 ?g in 8-35 ?l volume. Extensive automation has been made possible by the combination of database tools, mechanization of key process steps, and the use of a micro-cryoprobe that gives excellent data while requiring little optimization and manual setup. In this perspective, we describe the overall process used by the NESG for screening NMR samples as part of a sample optimization process, assessing optimal construct design and solution conditions, as well as for ...

2010-01-01

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Predation and fragmentation portrayed in the statistical structure of prey time series

Ecological speciation is the process by which barriers to gene flow between populations evolve due to adaptive divergence via natural selection. A relatively unexplored area in ecological speciation...Full Text Available

2010-09-01

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An ecological analysis of knowing by wielding.

BackgroundStatistical autoregressive analyses of direct and delayed density dependence are widespread in ecological research. The models suggest that changes in ecological factors...Full Text Available

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Toxicological benchmarks for screening contaminants of potential concern for effects on freshwater biota

The ecological approach to perception, as developed by James Gibson, is described and applied to how one knows, by means of the haptic perceptual system, various properties of hand-held objects. Four...Full Text Available

1989-11-01

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Ongoing collapse of coral-reef shark populations.

An important early step in the assessment of ecological risks at contaminated sites is the screening of chemicals detected on the site to identify those that constitute a potential risk. Part of this screening process is the comparison of measured ambient concentrations to concentrations that are believed to be nonhazardous, termed benchmarks. This article discusses 13 methods by which benchmarks may be derived for aquatic biota and presents benchmarks for 105 chemicals. It then compares them with respect to their sensitivity, availability, magnitude relative to background concentrations, and conceptual bases. This compilation is limited to chemicals that have been detected on the US Department of Energy's Oak Ridge Reservation (ORR) and to benchmarks derived from studies of toxic effects on freshwater organisms. The list of chemicals includes 45 metals and 56 industrial organic chemicals but only four pesticides. Although some individual ...

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The ecology of software configuration management

Marine ecosystems are suffering severe depletion of apex predators worldwide; shark declines are principally due to conservative life-histories and fisheries overexploitation. On coral reefs, sharks are strongly interacting apex predators and play a key role in maintaining healthy reef ecosystems. Despite increasing fishing pressure, reef shark catches are rarely subject to specific limits, with management approaches typically depending upon no-take marine reserves to maintain populations. Here, we reveal that this approach is failing by documenting an ongoing collapse in two of the most abundant reef shark species on the Great Barrier Reef (Australia). We find an order of magnitude fewer sharks on fished reefs compared to no-entry management zones that encompass only 1% of reefs. No-take zones, which are more difficult to enforce than no-entry zones, offer almost no protection for shark populations. Population viability models of whitetip and gray reef sharks project ongoing steep ...

2006-12-01

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Studies on Vegetation for Ecological Restoration of Salt Marshes in Saemangeum Reclaimed Land - Germination Strategies and Character of Halophytes -

This paper discusses how to overcome failures and pitfalls in software configuration management. (LSP)

1988-01-01

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Simon A. Levin's Passion for Ecology

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2009-04-01

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Meat-Fat Tail Breed of Sheep

... prominent example today, perhaps, is the issue of stem cell research, but there is a whole list of issues ... ...

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Environmental Effects on Oil Pipelines

Creation of Meat and Fat Tail Breed Sheep and Production of Ecologically Pure Mutton and Lamb

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`Ecological forestry' and eucalypt forests managed for wood production in south-western Australia

Determination of the Ecology Factor for the Magistral Oil Pipelines, Working in Difficult Hilly and Geology Conditions

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British Library Electronic Table of Contents (United Kingdom)

The model of `ecological forestry' has evolved as a part of the development of the concept of ecosystem management. `Ecological forestry' emphasises that manipulation of a forest ecosystem should consider, and as far as practicable work within the limits of, natural disturbance patterns prior to extensive human alteration of the landscape. This paper evaluates the extent to which forest management practices in jarrah (Eucalyptus marginata) and karri (Eucalyptus diversicolor) forests of south-western Australia align with this view of the characteristics and appropriate silviculture of `ecological forestry'. Characteristics and appropriate silviculture of `ecological forestry' are evaluated in relation to (i) the stand level decisions of stand structure and harvest timing and (ii) the landsc...

2007-01-01

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The mode and tempo of genome size evolution in eukaryotes

Sequence space coverage, entropy of genomes and the potential to detect non-human DNA in human samples

Eukaryotic genome size varies over five orders of magnitude; however, the distribution is strongly skewed toward small values. Genome size is highly correlated to a number of phenotypic traits, suggesting...Full Text Available

2007-05-01

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Massive turnover of functional sequence in human and other mammalian genomes

BackgroundGenomes store information for building and maintaining organisms. Complete sequencing of many genomes provides the opportunity to study and compare global information properties...Full Text Available

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Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies

Despite the availability of dozens of animal genome sequences, two key questions remain unanswered: First, what fraction of any species' genome confers biological function, and second, are apparent...Full Text Available

2010-10-01

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Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum

The spliced alignment of expressed sequence data to genomic sequence has proven a key tool in the comprehensive annotation of genes in eukaryotic genomes. A novel algorithm was developed to assemble...Full Text Available

2003-10-01

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Full genome gene expression analysis of the heat stress response in Drosophila melanogaster

The genome sequence of the solvent-producing bacterium Clostridium acetobutylicum ATCC 824 has been determined by the shotgun approach. The genome consists of a 3.94-Mb chromosome and...Full Text Available

2001-08-01

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Extensive synteny conservation of holocentric chromosomes in Lepidoptera despite high rates of local genome rearrangements

The availability of full genome sequences has allowed the construction of microarrays, with which screening of the full genome for changes in gene expression is possible. This method can provide a wealth...Full Text Available

2005-10-01

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A DNA transposon-based approach to validate oncogenic mutations in the mouse

The recent assembly of the silkworm Bombyx mori genome with 432 Mb on 28 holocentric chromosomes has become a reference in the genomic analysis of the very diverse Order of Lepidoptera....Full Text Available

2010-04-27

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A Computational Framework Discovers New Copy Number Variants with Functional Importance

Large-scale cancer genome projects will soon be able to sequence many cancer genomes to comprehensively identify genetic changes in human cancer. Genome-wide association studies have also identified...Full Text Available

2008-12-16

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Widespread duplications in the genomes of laboratory stocks of Dictyostelium discoideum

Structural variants which cause changes in copy numbers constitute an important component of genomic variability. They account for 0.7% of genomic differences in two individual genomes, of which...Full Text Available

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Time-dependent ARMA modeling of genomic sequences

BackgroundDuplications of stretches of the genome are an important source of individual genetic variation, but their unrecognized presence in laboratory organisms would be a confounding...Full Text Available

2008-01-01

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Methods in DNA methylation profiling

BackgroundOver the past decade, many investigators have used sophisticated time series tools for the analysis of genomic sequences. Specifically, the correlation of the nucleotide...Full Text Available

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Genomics of human longevity

Metastable and somatically heritable patterns of DNA methylation provide an important level of genomic regulation. In this article, we review methods for analyzing these genome-wide epigenetic...Full Text Available

2009-12-01

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Genomic view of the evolution of the complement system

In animal models, single-gene mutations in genes involved in insulin/IGF and target of rapamycin signalling pathways extend lifespan to a considerable extent. The genetic, genomic and epigenetic influences...Full Text Available

2011-01-12

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Genomic imprinting and the social brain

The recent accumulation of genomic information of many representative animals has made it possible to trace the evolution of the complement system based on the presence or absence of each complement...Full Text Available

2006-09-01

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Genetics, Genomics, and Molecular Biology

Genomic imprinting refers to the parent-of-origin-specific epigenetic marking of a number of genes. This epigenetic mark leads to a bias in expression between maternally and paternally inherited imprinted...Full Text Available

2006-12-29

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Enzymatic engineering of the porcine genome with transposons and recombinases

Genetics, Genomics, and Molecular Biology USGS scientists develop and integrate new genetic and molecular techniques into systematic analyses to describe individuals and populations of fish .....

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Comparative and phylogenomic studies on the mitochondrial genomes of Pentatomomorpha (Insecta: Hemiptera: Heteroptera)

BackgroundSwine is an important agricultural commodity and biomedical model. Manipulation of the pig genome provides opportunity to improve production efficiency, enhance disease...Full Text Available

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Cancer gene discovery in mouse and man

BackgroundNucleotide sequences and the gene arrangements of mitochondrial genomes are effective tools for resolving phylogenetic problems. Hemipteroid insects are known to possess...Full Text Available

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Ecological footprint and major driving forces in West Jilin Province, Northeast China

AbstractThe elucidation of the human and mouse genome sequence and developments in high-throughput genome analysis, and in computational tools, have made it possible to profile entire...Full Text Available

2009-12-01

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British Library Electronic Table of Contents (United Kingdom)

The environmental impact caused by local people (ecological footprint of consumption, EFc) and the actual environmental impact that the ecosystem burdens (ecological footprint of production, EFp) in West Jilin Province, Northeast China from 1986 to 2006 were evaluated by using ecological footprint (EF) method. And the major driving forces of EFc and EFp were analyzed by STIRPAT model. Both EFc and EFp showed increasing trends in 1986?2006, accompanied by decreasing ecological deficits but expanding ecological overshoots. Population (P), GDP per capita (A 1), quadratic term of GDP per capita (A 2), urbanization (T a1), and quadratic term of urbanization (T a2) were important influencing factors of EFc, among which T a2 and T a1 were the most dominate driving forces of EFc. A 1, A 2 and T a2...

2010-01-01

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STATE-OF-THE-ART AND EMERGING TRUCK ENGINE TECHNOLOGIES FOR OPTIMIZED PERFORMANCE, EMISSIONS AND LIFE CYCLE COSTS

Expression and Characterization of a New Esterase Cloned Directly from Agrobacterium tumefaciens Genome

The challenge for truck engine product engineering is not only to fulfill increasingly stringent emission requirements, but also to improve the engine's economical viability in its role as the backbone of our global economy. While societal impact and therefore emission limit values are to be reduced in big steps, continuous improvement is not enough but technological quantum leaps are necessary. The introduction and refinement of electronic control of all major engine systems has already been a quantum leap forward. Maximizing the benefits of these technologies to customers and society requires full use of parameter optimization and other enabling technologies. The next big step forward will be widespread use of exhaust aftertreatment on all transportation related diesel engines. While exhaust gas aftertreatment has been successfully established on gasoline (Otto cycle) engines, the introduction of exhaust aftertreatment especially for ...

2003-08-24

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RNA:DNA Ratio and Other Nucleic Acid Derived Indices in Marine Ecology

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2006-01-01

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High genetic variability and low local diversity in a population of arbuscular mycorrhizal fungi

Some of most used indicators in marine ecology are nucleic acid-derived indices. They can be divided by target levels in three groups: 1) at the organism level as ecophysiologic indicators, indicators...Full Text Available

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SNIFFER - UKTAG partnership supporting the

Arbuscular mycorrhizal fungi (AMF) are ecologically important root symbionts of most terrestrial plants. Ecological studies of AMF have concentrated on differences between species; largely assuming...Full Text Available

2004-02-24

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Ecologically clean prophylactic food addition from eatable mushroom

to set in place regulations and processes to manage river basins based upon the ecological (and hydro-morphological) health

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Justice and the Human Genome Project

... Safety Institute, GRS, Cologne (Germany) INIS-UA--089 456 p. APPLIED LIFE

2001-04-18

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Interpreting Mammalian Evolution using Fugu Genome Comparisons

Most of the essays gathered in this volume were first presented at a conference, Justice and the Human Genome, in Chicago in early November, 1991. The goal of the, conference was to consider questions of justice as they are and will be raised by the Human Genome Project. To achieve its goal of identifying and elucidating the challenges of justice inherent in genomic research and its social applications the conference drew together in one forum members from academia, medicine, and industry with interests divergent as rate-setting for insurance, the care of newborns, and the history of ethics. The essays in this volume address a number of theoretical and practical concerns relative to the meaning of genomic research.

1992-01-01

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Industrial ecology at Lawrence Livermore National Laboratory summary statement

Comparative sequence analysis of the human and the pufferfish Fugu rubripes (fugu) genomes has revealed several novel functional coding and noncoding regions in the human genome. In particular, the fugu genome has been extremely valuable for identifying transcriptional regulatory elements in human loci harboring unusually high levels of evolutionary conservation to rodent genomes. In such regions, the large evolutionary distance between human and fishes provides an additional filter through which functional noncoding elements can be detected with high efficiency.

2004-04-02

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The use of molecular biology techniques for the diagnosis and epidemiological study of foot-and-mouth disease virus in Thailand

At Livermore our hope and our intention is to make important contributions to global sustainability by basing both our scientific and technological research and our business practices on the principles of industrial ecology. Current efforts in the following fields are documented: global security, global ecology, energy for transportation, fusion energy, materials sciences, environmental technology, and bioscience.

1996-06-04

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ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

The detection of foot-and-mouth disease (FMD) virus from various kinds of field samples (tissue extract and cell culture isolate) was studied using the polymerase chain reaction (PCR) technique. The gene selected for diagnosis was the polymerase gene and an amplification target product of 454 bp in length was produced using AP5/AP6 primer sets. The PCR product was further examined by NcoI endonuclease digestion. The presence of the internal restriction site was confirmed by demonstration of two small fragments of 330 bp and 124 bp in length. Forty-nine samples that gave positive and negative results by ELISA typing and were positive by the PCR test were tested by NcoI digestion to confirm the results. About 10% of PCR products could not be confirmed by the method. Furthermore the FMD RNA polymerase gene could be detected by the PCR method in samples negative in both ELISA typing and the virus isolation test. A total of 23 samples were examined and compared after each stage of the ...

2000-05-01

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The carbonate feedback system: interaction between stratigraphic accommodation, ecological succession and the carbonate factory; Le systeme de feed-back des carbonates: une interaction entre accomodation stratigraphique, succession ecologique et l`usine des carbonates

Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. We have created a database of evolutionary conserved regions (ECRs) in vertebrate genomes entitled ECRbase that is constructed from a collection of pairwise vertebrate genome alignments produced by the ECR Browser database. ECRbase features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a collection of promoters in all vertebrate genomes presented in the database. The database also contains a collection of annotated transcription factor binding sites (TFBS) in all ECRs and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and two pufferfish genomes. It is freely accessible at http://ECRbase.dcode.org.

2006-08-08

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Whole-genome cancer analysis as an approach to deeper understanding of tumour biology

In this paper, the analysis of carbonate depositional systems, using high resolution sequence stratigraphy, studies the ratio between change in accommodation space and skeletal carbonate production. This approach establishes the concurrent change in nature of facies belts (expansion, retraction and type) and fossil communities, within the detailed time frame in accommodation. Carbonate production is generally considered to be a fairly simple function of environmental conditions such as climate and water depth. However, data from several case studies also show a covariance between change in stratigraphic architecture of carbonate systems (the seaward or landward stepping stacking patterns of stratigraphic units) and charge in facies. Case studies include Carboniferous algal mound development in the Paradox Basin (USA), Devonian stromatoporoid reef development in Alberta (Canada), and Cenomanian rudistid shoal development in Oman. The hypotheses are tested on cases ...

1996-12-31

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Using Regulatory and Epistatic Networks to Extend the Findings of a Genome Scan: Identifying the Gene Drivers of Pigmentation in Merino Sheep

Recent advances in DNA sequencing technology are providing unprecedented opportunities for comprehensive analysis of cancer genomes, exomes, transcriptomes, as well as epigenomic components. The integration...Full Text Available

2010-01-19

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Transcriptional mapping of the 3' end of the bovine syncytial virus genome.

Extending genome wide association analysis by the inclusion of gene expression data may assist in the dissection of complex traits. We examined piebald, a pigmentation phenotype in both human and Merino...Full Text Available

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The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

The bovine syncytial virus, a member of the retroviral subfamily Spumavirinae, causes a persistent, asymptomatic infection in cattle. Nucleotide sequence analysis of the viral genome revealed two overlapping...Full Text Available

1994-02-01

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Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase

BackgroundFood supply from the ocean is constrained by the shortage of domesticated and selected fish. Development of genomic models of economically important fishes should assist...Full Text Available

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Synthesis and degradation of dinoflagellate plastid-encoded psbA proteins are light-regulated, not circadian-regulated

The development of new methods for gene addition to mammalian genomes is necessary to overcome the limitations of conventional genetic engineering strategies. Although a variety of DNA-modifying enzymes...Full Text Available

2011-09-01

77

Replication Stress Induces Genome-wide Copy Number Changes in Human Cells that Resemble Polymorphic and Pathogenic Variants

In many dinoflagellate species, the plastid genome has been proposed to exist as a limited number of single-gene minicircles, and many genes normally found in the plastid genome are nuclear-encoded....Full Text Available

2005-02-22

78

Reciprocal Silencing, Transcriptional Bias and Functional Divergence of Homeologs in Polyploid Cotton (Gossypium)

Copy number variants (CNVs) are an important component of genomic variation in humans and other mammals. Similar de novo deletions and duplications, or copy number changes (CNCs), are now known to be...Full Text Available

2009-03-13

79

Rai1 duplication causes physical and behavioral phenotypes in a mouse model of dup(17)(p11.2p11.2)

Polyploidy is an important force in the evolution of flowering plants. Genomic merger and doubling induce an extensive array of genomic effects, including immediate and long-term alterations in the...Full Text Available

2009-06-01

80

Rab protein evolution and the history of the eukaryotic endomembrane system

Genomic disorders are conditions that result from DNA rearrangements, such as deletions or duplications. The identification of the dosage-sensitive gene(s) within the rearranged genomic interval is...Full Text Available

2006-11-01

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81

Presence of two independent chromosomes in the Brucella melitensis 16M genome.

Spectacular increases in the quantity of sequence data genome have facilitated major advances in eukaryotic comparative genomics. By exploiting homology with classical model organisms, this makes possible...Full Text Available

2010-10-01

82

Parallel Loss of Plastid Introns and Their Maturase in the Genus Cuscuta

Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two...Full Text Available

1993-02-01

83

Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging

Plastid genome content and arrangement are highly conserved across most land plants and their closest relatives, streptophyte algae, with nearly all plastid introns having invaded the genome in their...Full Text Available

84

Natural mutagenesis of human genomes by endogenous retrotransposons

The nucleocapsid protein (NC) of retroviruses plays a major role in genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA binding. To further...Full Text Available

1998-03-01

85

Molecular characterization of a Chinese variant of the Flury-LEP strain

SUMMARYTwo abundant classes of mobile elements, namely Alu and L1 elements, continue to generate new retrotransposon insertions in human genomes. Estimates suggest that these...Full Text Available

2010-06-25

86

Mobilizing diversity: transposable element insertions in genetic variation and disease

The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was...Full Text Available

87

Large-scale cross-species oncogenomics identifies candidate oncogenes and tumor suppressor genes

Transposable elements (TEs) comprise a large fraction of mammalian genomes. A number of these elements are actively jumping in our genomes today. As a consequence, these insertions provide a source...Full Text Available

88

Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera

While genomic alterations identified in human tumors using techniques such as comparative genomic hybridisation (CGH) may be recurrent, they frequently encompass large regions, in some cases...Full Text Available

2010-02-01

89

Integration of metabolic databases for the reconstruction of genome-scale metabolic networks

Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing...Full Text Available

2011-01-01

90

Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence

BackgroundGenome-scale metabolic reconstructions have been recognised as a valuable tool for a variety of applications ranging from metabolic engineering to evolutionary studies....Full Text Available

91

Impact of genetic changes to the CRPV genome and their application to the study of pathogenesis in vivo

The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically...Full Text Available

2005-11-01

92

The cottontail rabbit papillomavirus (CRPV)/rabbit model has been used to study oncogenicity and immunogenicity of different antigens from the papillomavirus genome and has therefore served...Full Text Available

2007-02-20

93

Genomic cloning and characterization of a ricin gene from Ricinus communis.

BackgroundA large fraction of the human genome is attributable to L1 retrotransposon sequences. Not only do L1s themselves make up a significant portion of the genome, but L1-encoded...Full Text Available

2003-01-01

94

Genomes are covered with ubiquitous 11 bp periodic patterns, the "class A flexible patterns"

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor...Full Text Available

1985-11-25

95

Genome-wide detection and characterization of positive selection in human populations

BackgroundThe genomes of prokaryotes and lower eukaryotes display a very strong 11 bp periodic bias in the distribution of their nucleotides. This bias is present throughout a given...Full Text Available

96

Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.)

With the advent of dense maps of human genetic variation, it is now possible to detect positive natural selection across the human genome. Here we report an analysis of over 3 million polymorphisms...Full Text Available

2007-10-18

97

Genome-Wide Identification and Evolutionary Analysis of the Animal Specific ETS Transcription Factor Family

BackgroundCucumber, Cucumis sativus L. is an important vegetable crop worldwide. Until very recently, cucumber genetic and genomic resources, especially molecular...Full Text Available

98

Genome size and wing parameters in passerine birds

The ETS proteins are a family of transcription factors (TFs) that regulate a variety of biological processes. We made genome-wide analyses to explore the classification of the ETS gene family. We identified...Full Text Available

99

Genetic susceptibility to systemic lupus erythematosus in the genomic era

Despite their status as the most speciose group of terrestrial vertebrates, birds exhibit the smallest and least variable genome sizes among tetrapods. It has been suggested that this is because powered...Full Text Available

2009-01-07

100

Estimating Missing Heritability for Disease from Genome-wide Association Studies

Our understanding of the genetic basis of systemic lupus erythematosus (SLE) has been rapidly advanced using large-scale, case–control, candidate gene studies as well as genome-wide...Full Text Available

2010-12-01

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101

Epitope tagging of endogenous proteins for genome wide Chromatin immunoprecipitation analysis

Genome-wide association studies are designed to discover SNPs that are associated with a complex trait. Employing strict significance thresholds when testing individual SNPs avoids false positives at...Full Text Available

2011-03-11

102

Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean1[C][W

The development of chromatin immunoprecipitation methods coupled with DNA microarray (ChIP-chip) technology has enabled genome-wide identification of cis-DNA regulatory elements to which transcription...Full Text Available

2009-01-01

103

Completely phased genome sequencing through chromosome sorting

Soybean (Glycine max) is a paleopolyploid whose genome has gone through at least two rounds of polyploidy and subsequent diploidization events. Several studies have investigated the...Full Text Available

2009-11-01

104

Complete plastid genome sequences suggest strong selection for retention of photosynthetic genes in the parasitic plant genus Cuscuta

The two haploid genome sequences that a person inherits from the two parents represent the most fundamentally useful type of genetic information for the study of heritable diseases and the development...Full Text Available

2011-01-04

105

Comparative genomics of the bacterial genus Listeria: Genome evolution is characterized by limited gene acquisition and limited gene loss

BackgroundPlastid genome content and protein sequence are highly conserved across land plants and their closest algal relatives. Parasitic plants, which obtain some or all of their...Full Text Available

106

Bunyamwera virus can repair both insertions and deletions during RNA replication

BackgroundThe bacterial genus Listeria contains pathogenic and non-pathogenic species, including the pathogens L. monocytogenes and L. ivanovii,...Full Text Available

107

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

The genomic termini of RNA viruses contain essential cis-acting signals for such diverse functions as packaging, genome translation, mRNA transcription, and RNA replication, and thus...Full Text Available

2010-06-01

108

Behavioral genomics of honeybee foraging and nest defense

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have...Full Text Available

2010-10-01

109

Avian Nephritis Virus (ANV) as a New Member of the Family Astroviridae and Construction of Infectious ANV cDNA

The honeybee has been the most important insect species for study of social behavior. The recently released draft genomic sequence for the bee will accelerate honeybee behavioral genetics. Although...Full Text Available

2007-04-01

110

Analysis of illegitimate genomic integration mediated by zinc-finger nucleases: implications for specificity of targeted gene correction

The complete RNA genome of the avian nephritis virus (ANV) associated with acute nephritis in chickens has been molecularly cloned and sequenced. Excluding the poly(A) tail, the genome comprises 6,927...Full Text Available

2000-09-01

111

A proposal to sequence the genome of a garter snake (Thamnophis sirtalis)

BackgroundFormation of site specific genomic double strand breaks (DSBs), induced by the expression of a pair of engineered zinc-finger nucleases (ZFNs), dramatically increases the...Full Text Available

112

A haplotype map of the human genome

Here we develop an argument in support of sequencing a garter snake (Thamnophis sirtalis) genome, and outline a plan to accomplish this. This snake is a common, widespread, nonvenomous...Full Text Available

113

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish

Inherited genetic variation has a critical but as yet largely uncharacterized role in human disease. Here we report a public database of common variation in the human genome: more than one million...Full Text Available

2005-10-27

114

A Marker-Dense Physical Map of the Bradyrhizobium japonicum Genome

BackgroundThe genomic organisation of the Major Histocompatibility Complex (MHC) varies greatly between different vertebrates. In mammals, the classical MHC consists of a large number...Full Text Available

115

Genomic Careers: Interactive Videos

Bacterial artificial chromosome (BAC) clones are effective mapping and sequencing reagents for use with a wide variety of small and large genomes. This report describes the development of a physical...Full Text Available

2001-08-01

116

Medline Plus

... the nature of DNA testing. 07:56 - President / CEO of a Biotechnology / Pharmaceutical Company - Sherri Bale President ...

117

Exons, Introns and Talking Genes: The Sience Behind the Human Genome Project

Genome-Wide Association Study SNPs in the Human Genome Diversity Project Populations: Does Selection Affect Unlinked SNPs with Shared Trait Associations?

This book presents in simple terms the basis of molecular genetics and how it is used to obtain an understanding of the human genome. The author's central focus is the transistion of genetics from statistics to experimental manipulations, and he offers analogies that help readers visualize the genome, thereby avoiding conventional scientific presentations. He illustrates how genetics is used in scientific laboratories, in courtrooms, and in hospitals. Little is presented about the complex social and ethical issues raised by the Human Genome project.

1993-01-01

118

Genome structure of cotton revealed by a genome-wide SSR genetic map constructed from a BC₁population between gossypium hirsutum and G. barbadense

Genome-wide association studies (GWAS) have identified more than 2,000 trait-SNP associations, and the number continues to increase. GWAS have focused on traits with potential consequences for human...Full Text Available

2011-01-01

119

An ecologically relevant exposure assessment for a polluted river using an integrated multivariate PLS approach

BackgroundCotton, with a large genome, is an important crop throughout the world. A high-density genetic linkage map is the prerequisite for cotton genetics and breeding. A genetic...Full Text Available

120

Hanford Federal Facility Agreement and Consent Order quarterly progress report for the period ending June 30, 1991

A case study is presented where an integrated, ecologically relevant exposure assessment is presented for a polluted lowland river. Using partial least squares regression of latent structures (PLS), an analysis of the impact of two effluents on physico-chemical water quality measures, macroinvertebrate and diatom communities, and in situ bioassay responses with four different test species are combined into an integrative exposure assessment. Bioassays focussed on growth and condition related endpoints, because they are key functional processes of organisms and populations. Integrating these multiple lines of evidence, we were able to discriminate among the impact of both effluents, link changes in physico-chemical water quality with bioassay endpoints and ecological quality of the ecosystem, and address the importance of integrating all information into one exposure assessment framework. The bioassays under field conditions indicated that most ...

2004-11-01

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121

Structure, Function, and Evolution of Rice Centromeres

This is the ninth quarterly report as required by the Hanford Federal Facility Agreement and Consent Order (Ecology et al. 1990), also known as the Tri-Party Agreement, established between the US Department of Energy (DOE), the US Environmental Protection Agency (EPA), and the Washington State Department of Ecology (Ecology). The Tri-Party Agreement sets the plan and schedule for achieving regulatory compliance and cleanup of waste sites at the Hanford Site. This report covers progress for the quarter that ended June 30, 1991. A total of 87 milestones have been completed to date. 39 refs., 1 fig.

1991-08-01

122

Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase

The centromere is the most characteristic landmark of eukaryotic chromosomes. Centromeres function as the site for kinetochore assembly and spindle attachment, allowing for the faithful pairing and segregation of sister chromatids during cell division. Characterization of centromeric DNA is not only essential to understand the structure and organization of plant genomes, but it is also a critical step in the development of plant artificial chromosomes. The centromeres of most model eukaryotic species, consist predominantly of long arrays of satellite DNA. Determining the precise DNA boundary of a centromere has proven to be a difficult task in multicellular eukaryotes. We have successfully cloned and sequenced the centromere of rice chromosome 8 (Cen8), representing the first fully sequenced centromere from any multicellular eukaryotes. The functional core of Cen8 spans ~800 kb of DNA, which was determined by chromatin immunoprecipitation ...

2010-02-04

123

Comparing compressed sequences for faster nucleotide BLAST searches.

By catalyzing the rate-limiting step in adipose tissue lipolysis, hormone-sensitive lipase (HSL) is an important regulator of energy homeostasis. The role and importance of HSL in tissues other than adipose are poorly understood. We report here the cloning and expression of a testicular isoform, designated HSL{sub tes}. Due to an addition of amino acids at the NH{sub 2}-termini, rat and human HSL{sub tes} consist of 1068 and 1076 amino acids, respectively, compared to the 768 and 775 amino acids, respectively, of the adipocyte isoform (HSL{sub adi}). A novel exon of 1.2 kb, encoding the human testis-specific amino acids, was isolated and mapped to the HSL gene, 16 kb upstream of the exons encoding HSL{sub adi}. The transcribed mRNA of 3.9 kb was specifically expressed in testis. No significant similarity with other known proteins was found for the testis-specific sequence. The amino acid composition differs from the HSL{sub adi} sequence, with a notable hydrophilic ...

1996-08-01

124

A human breast cell model of pre-invasive to invasive transition

Molecular biologists, geneticists, and other life scientists use the BLAST homology search package as their first step for discovery of information about unknown or poorly annotated genomic sequences. There are two main variants of BLAST: BLASTP for searching protein collections and BLASTN for nucleotide collections. Surprisingly, BLASTN has had very little attention; for example, the algorithms it uses do not follow those described in the 1997 BLAST paper and no exact description has been published. It is important that BLASTN is state-of-the-art: Nucleotide collections such as GenBank dwarf the protein collections in size, they double in size almost yearly, and they take many minutes to search on modern general purpose workstations. This paper proposes significant improvements to the BLASTN algorithms. Each of our schemes is based on compressed bytepacked formats that allow queries and collection sequences to be compared four bases at a time, ...

125

Improvement of the parameters of shallow p"+-n-junctions in silicon by additional carbon implantation and step-by-step thermal treatments

A crucial step in human breast cancer progression is the acquisition of invasiveness. There is a distinct lack of human cell culture models to study the transition from pre-invasive to invasive phenotype as it may occur 'spontaneously' in vivo. To delineate molecular alterations important for this transition, we isolated human breast epithelial cell lines that showed partial loss of tissue polarity in three-dimensional reconstituted-basement membrane cultures. These cells remained non-invasive; however, unlike their non-malignant counterparts, they exhibited a high propensity to acquire invasiveness through basement membrane in culture. The genomic aberrations and gene expression profiles of the cells in this model showed a high degree of similarity to primary breast tumor profiles. The xenograft tumors formed by the cell lines in three different microenvironments in nude mice displayed metaplastic phenotypes, including ...

2008-03-10

126

Use of radionuclide X-ray fluorescence analysis by Institute of Industrial Landscape Ecology of Czechoslovak Academy of Sciences, Ostrava

In this article carbon co-implantation and step-by-step thermal treatments of shallow p"+-n-junctions formation were used with the purpose of extended defect suppression and reduction of boron transient enhanced diffusion. A substantial improvement of the structural and electrical parameters of shallow p"+-n-junctions has been achieved by using the additional carbon implantation and step-by-step thermal treatments. (authors)

127

Two sisters in the same dress: Heliconius cryptic species

Czech 1985. p. 48. Czechoslovakia Sikora, J. Ustav Ekologie Prumyslove

1985-06-10

128

Science and technology for industrial ecology

BackgroundSister species divergence and reproductive isolation commonly results from ecological adaptation. In mimetic Heliconius butterflies, shifts in colour pattern...Full Text Available

129

Overview of geology, hydrology, geomorphology, and sediment ...

This paper first discusses the challenge offered by natural and anthropogenic systems in all of their complexity and then indicates some areas of research in which specific scientific and technological needs are identifiable.

1996-07-10

130

Mangroves in the Gulf of California increase fishery yields

Jun 16, 2011 ... Description: Within the Deschutes River basin of central Oregon, the geology, hydrology, and physiography influence geomorphic and ecologic ...

131

Ecology of Vibrio mimicus in aquatic environments.

Mangroves are disappearing rapidly worldwide despite their well documented biodiversity and the ecosystem services they provide. Failure to link ecological processes and their societal benefits has...Full Text Available

2008-07-29

132

Ecological utilization of wastes. A manual for optimum waste management concepts. 3. rev. ed. Oekologische Muellverwertung. Handbuch fuer optimale Abfall-Konzepte

An environmental study was done to examine the prevalence of Vibrio mimicus in some aquatic environments of Dhaka, Bangladesh, and of Okayama, Japan. Water samples from Dhaka environments and water...Full Text Available

1989-08-01

133

Diagnostics of Radionuclides Effects Results

From out of the numerous technical alternatives the manual develops a waste management concept with emphasis on methods which can be coordinated for maximum ecological use and minimum economic costs. A clear-cut analysis of the present state of waste utilization and waste disposal in the Federal Republic of Germany (chapter 1) is followed by a detailed description of conventional and modern state-of-the-art waste utilization methods based on ecological evaluation criteria (chapter 3). An optimum waste utilization concept for defined quantities of wastes is derived from the ecological and economic comparison of waste utilization techniques given in chapter 3. Chapter 4 points out different variants of waste utilization and waste disposal methods and shows how to determine the optimum variant of the optimum concept for a particular area or community. (orig.).

1991-01-01

134

Development of an Adaptive Tsetse Population Management Scheme for the Luke Community, Ethiopia

Development of New Methods and Means of Assessing of Consequences of Radionuclide and Heavy Metal Salt Effect, Criteria of Forecasting Physiological State and Productivity of the Farm Animals under Conditions of Ecological Pollution of Environment

135

Chronic toxicity of environmental contaminants: sentinels and biomarkers.

... African agro-pastoral system: Management of tsetse and bovine trypanosomiasis. Ecological Economics 65:1, 125-135Online publication date: ... ...

136

2010 NASA Terrestrial Ecology Science Team Meeting - NASA Carbon ...

Due to the use of a limited number of species and subchronic exposures, current ecological hazard assessment processes can underestimate the chronic toxicity of environmental contaminants resulting...Full Text Available

1997-02-01

137

Survey and analysis of simple sequence repeats in the Laccaria bicolor genome, with development of microsatellite markers

The biomass maps were compared with the U.S. Forest Service biomass map for 2002 , LVIS height data, and estimates from high resolution imagery. ...

138

Utilization of plastic wastes in a blast furnace - a contribution to ecological and economical recycling of plastic wastes; Kunststoffverwertung im Hochofen - ein Beitrag zum oekologischen und oekonomischen Recycling von Altkunststoffen

It is becoming clear that simple sequence repeats (SSRs) play a significant role in fungal genome organization, and they are a large source of genetic markers for population genetics and meiotic maps. We identified SSRs in the Laccaria bicolor genome by in silico survey and analyzed their distribution in the different genomic regions. We also compared the abundance and distribution of SSRs in L. bicolor with those of the following fungal genomes: Phanerochaete chrysosporium, Coprinopsis cinerea, Ustilago maydis, Cryptococcus neoformans, Aspergillus nidulans, Magnaporthe grisea, Neurospora crassa and Saccharomyces cerevisiae. Using the MISA computer program, we detected 277,062 SSRs in the L. bicolor genome representing 8% of the assembled genomic sequence. Among the analyzed basidiomycetes, L. bicolor exhibited the highest SSR density although no correlation ...

2011-01-01

139

The ecological role of bacteriocins in bacterial competition.

This article describes the utilization of plastic wastes in a blast furnace. The plastic waste is a substitution for petroleum and coal as a raw material for synthesis gas production. The synthesis gas is the reducing agent in the blast furnace for the reduction of iron ores. You can find here an ecological and economical analysis of this process in comparison to the utilization of petroleum and coal. (SR)

1996-12-31

140

The application of ecosystems services criteria for green building assessment

Bacteriocins are an abundant class of antimicrobial molecules that appear to mediate population dynamics within species. The bacteriocins of Escherichia coli have served as a model for exploring the ecological role of these potent toxins. Studies suggest that colicins provide a competitive edge in nutrient-poor environments and that there might be a trade-off between the costs and benefits of colicin production. PMID:10203843

1999-03-01

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141

Technical-ecologic principles of efficient use of open pit mine motor transport

In the discussion of environmental architecture, we are conjoining two disciplines, the subject of architecture and that of ecology. At their best, green buildings are examples of applied ecology, where designers understand the constitution, organization, and structure of ecosystems, and the impacts of architecture are considered from an environmental perspective. By utilizing the concepts, methods, and language of ecology, designers can create architecture that intentionally engages the natural systems of a site. The establishment of assessment criteria implies the definition of building design criteria. If we establish criteria that are based on our best scientific understanding of environmental capacity, we will begin to develop a building stock that is sustainable. To do this we must quantify the link between the resulting environmental impacts and their cause in building production and use. This is not done in ...

2004-10-01

142

Power and ecological aspects of hydrogen and hydrogenous gas usage

The effect of open pit mine motor transport with ICE on the air basin is examined using the example of shale mines and open pits of the production association ''Estonslanets''. Efficient areas of use of different types of open pit mine motor transport, diesel-trolley tracks and electric cars are determined based on technical-economic and ecologic feasibility.

1981-01-01

143

Nutrient Pollution of Coastal Rivers, Bays, and Seas

General trends of developing hydrogen power engineering and technology in Ukraine are considered. Based on a general level of power consumption and real opportunities for hydrogen production, a conclusion is derived that there are possibilities in the near future to partially replace conventional hydrocarbon fuels by hydrogen. Besides, developed technologies for burning hydrogenous gases and hydrogen-fuel systems when applied to transport installations allow one to improve essentially their power and ecological characteristics. 5 refs.

144

Environmental Sciences Division annual progress report for period ending September 30, 1981

This 'Issues in Ecology' article from the Ecological Society of America provides information about the consequences of nutrient enrichment along the US coast. It describes problems such as harmful algal blooms (HABs), anoxia, hypoxia, and dead zones. It explains which nutrients are involved and describes implications of excess nutrients in regions such as the Gulf of Mexico/Mississippi River basin. The article features several color photographs, maps, and diagrams.

145

Hydrologic analysis for ecological risk assessment of watersheds with abandoned mine lands

Research programs from the following sections and programs are summarized: aquatic ecology, environmental resources, earth sciences, terrestrial ecology, advanced fossil energy program, toxic substances program, environmental impacts program, biomass, low-level waste research and development program, US DOE low-level waste management program, and waste isolation program.

1982-04-01

146

Hydro power plants: Ecology and economy in harmony; Wasserkraftwerke: Oekologie und Oekonomie im Einklang

As part of on-going study of acid mine drainage (AMD), a comprehensive ecological risk assessment was conducted in the Leading Creek Watershed in southeast Ohio. The watershed is influenced by agriculture and active and abandoned coal-mining operations. This work presents a broad overview of several quantitative measures of hydrology and hydraulic watershed properties available for in risk assessment and evaluates their relation to metrics of ecology. Data analysis included statistical comparisons of metrics of ecology, ecotoxicology, water quality, and physically based parameters describing land use, geomorphology, flow, velocity, and particle size. A multiple regression analysis indicated that abandoned mining operations dominated impacts upon aquatic ecology. It also indicated low flow velocity measurements and a ratio of maximum velocity to average velocity at low flow where helpful in describing ...

1999-07-25

147

Ecological response of a multi-purpose river development project using macro-invertebrates richness and fish habitat value

The application of fossil fuels and the simultaneous dependency on fuel imports can only be decreased through consequent utilisation of efficient hydro power which is the most efficient way to avoid CO{sub 2} emissions. Only some decades ago hydro power plants were buildings erected according to met technical demands. In the 1990s rethinking started and the new designs took into account ecology and architecture. Maintenance and/or improvement of ecological efficiency play a decisive role. Experts of ecology and environmental protection of different faculties are involved right from the beginning when planning starts in order to obtain a maximum of nature compatibility and to develop efficient ecological accompanying and compensating measures. Landscape is being recultivated sustainably during construction activities in order to offer biota optimum conditions of living. Devices supporting fish migration ...

2010-07-01

148

Engineering Relative Compression of Genomes

It has been acknowledged that river morphology and hydrology have been intensively altered due to the anthropic demands in floodplain land use and management, flood protection, promotion of navigability or energy production. Rivers were transformed in water highways, having lost contact with their surrounding floodplain as well as the plethora of ecological processes and occupants once thriving in these ecotonal zones. The identification of this emerging threat of morphological and hydrological alteration on ecological integrity adds further complexity in the exploitation of hydrosystem resources. These resources are heavily coveted and guarded by different lobbies each having strategic views on future project development. Stakeholders may want to promote hydro-electricity, ecologists a natural reserve, communes may wish to have an increased flood protection and leisure promoters a nautical center. As a result, the proposition of a river ...

2002-04-01

149

Ultraconserved Elements: Analyses of Dosage Sensitivity, Motifs and Boundaries

Technology progress in DNA sequencing boosts the genomic database growth at faster and faster rate. Compression, accompanied with random access capabilities, is the key to maintain those huge amounts of data. In this paper we present an LZ77-style compression scheme for relative compression of multiple genomes of the same species. While the solution bears similarity to known algorithms, it offers significantly higher compression ratios at compression speed over a order of magnitude greater. One of the new successful ideas is augmenting the reference sequence with phrases from the other sequences, making more LZ-matches available.

2011-01-01

150

The Importance of Mitochondrial DNA in Aging and Cancer

Ultraconserved elements (UCEs) are sequences that are identical between reference genomes of distantly related species. As they are under negative selection and enriched near or in specific classes...Full Text Available

2008-12-01

151

The COG database: an updated version includes eukaryotes

Mitochondrial dysfunction has been implicated in premature aging, age-related diseases, and tumor initiation and progression. Alterations of the mitochondrial genome accumulate both in aging tissue...Full Text Available

152

Systems medicine: the future of medical genomics and healthcare

BackgroundThe availability of multiple, essentially complete genome sequences of prokaryotes and eukaryotes spurred both the demand and the opportunity for the construction of an...Full Text Available

153

Proteinortho: Detection of (Co-)orthologs in large-scale analysis

High-throughput technologies for DNA sequencing and for analyses of transcriptomes, proteomes and metabolomes have provided the foundations for deciphering the structure, variation and function of the...Full Text Available

154

Polymer Genomics: An Insight into Pharmacology and Toxicology of Nanomedicines 1

BackgroundOrthology analysis is an important part of data analysis in many areas of bioinformatics such as comparative genomics and molecular phylogenetics. The ever-increasing flood...Full Text Available

155

Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin

Synthetic polymers and nanomaterials display selective phenotypic effects in cells and in the body that affect signal transduction mechanisms involved in inflammation, differentiation, proliferation,...Full Text Available

2006-12-30

156

Open-source software accelerates bioinformatics

Eukaryotic genomes are packaged in two basic forms, euchromatin and heterochromatin. We have examined the composition and organization of Drosophila melanogaster heterochromatin in...Full Text Available

2011-02-01

157

Nucleomorph Ribosomal DNA and Telomere Dynamics in Chlorarachniophyte Algae

A report on the Wellcome Trust/Cold Spring Harbor Genome Informatics meeting, Cold Spring Harbor, USA, 7-11 May 2003.

2003-01-01

158

British Library Electronic Table of Contents (United Kingdom)

ABSTRACT. Chlorarachniophytes are enigmatic marine unicellular algae that acquired photosynthesis by secondary endosymbiosis. Chlorarachniophytes are unusual in that the nucleus of the engulfed algal cell (a green alga) persists in a miniaturized form, termed a nucleomorph. The nucleomorph genome of the model chlorarachniophyte, Bigelowiella natans CCMP621, is 373 kilobase pairs (kbp) in size, the smallest nuclear genome characterized to date. The B. natans nucleomorph genome is composed of three chromosomes, each with canonical eukaryotic telomeres and sub telomeric ribosomal DNA (rDNA) operons transcribed away from the chromosome end. Here we present the complete rDNA operon and telomeric region from the nucleomorph genome of Lotharella oceanica CCMP622, a newly characterized chlorarachn...

2010-01-01

159

Manipulating the Xenopus genome with transposable elements

Identification of pork derivatives in food products by species-specific polymerase chain reaction (PCR) for halal verification

The study of amphibian embryogenesis has provided important insight into the mechanisms of vertebrate development. The frog Xenopus laevis has been an important model of vertebrate...Full Text Available

2007-01-01

160

British Library Electronic Table of Contents (United Kingdom)

Pork identification in four types of food products, which are sausages and the casings, bread and biscuits, using species-specific polymerase chain reaction (PCR) detection of a conserved region in the mitochondrial (mt) 12S ribosomal RNA (rRNA) gene was developed. Genomic DNA of the food products were successfully extracted except for the casing samples, where no genomic DNA was detected. The extracted genomic DNA was then subjected to PCR amplification targeting the specific regions of the 12S rRNA gene. The genomic DNA from the food products were found to be of good quality and produced clear PCR products on the amplification of 12S rRNA gene of 387 base pairs (bp) from pork species. The species-specific PCR identification yielded excellent results for identification of pork derivatives...

2007-01-01

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161

Human endogenous retroviruses: transposable elements with potential ?

Human cDNA mapping using fluorescence in situ hybridization. Final progress report, April 1, 1994--July 31, 1997

Human endogenous retroviruses (HERVs) are a significant component of a wider family of retroelements that constitute part of the human genome. These viruses, perhaps representative of previous exogenous...Full Text Available

2004-10-01

162

Genome analysis with inter-nucleotide distances

The ultimate goal of this research is to generate and apply novel technologies to speed completion and integration of the human genome map and sequence with biomedical problems. To do this, techniques were developed and genome-wide resources generated. This includes a genome-wide Mapped and Integrated BAC/PAC Resource that has been used for gene finding, map completion and anchoring, breakpoint definition and sequencing. In the last period of the grant, the Human Mapped BAC/PAC Resource was also applied to determine regions of human variation and to develop a novel paradigm of primate evolution through to humans. Further, in order to more rapidly evaluate animal models of human disease, a BAC Map of the mouse was generated in collaboration with the MTI Genome Center, Dr. Bruce Birren.

1997-12-31

163

Extracellular Signaling through the Microenvironment: A Hypothesis Relating Carcinogenesis, Bystander Effects, and ...

Motivation: DNA sequences can be represented by sequences of four symbols, but it is often useful to convert the symbols into real or complex numbers for further analysis. Several mapping...Full Text Available

2009-12-01

164

Evaluating Phylogenetic Congruence in the Post-Genomic Era

... Extracellular Signaling through the Microenvironment: A Hypothesis Relating Carcinogenesis, Bystander Effects, and Genomic InstabilityMary Helen Barcellos-Hoff1a ... ...

165

Developmentally programmed endoreduplication in animals

Congruence is a broadly applied notion in evolutionary biology used to justify multigene phylogeny or phylogenomics, as well as in studies of coevolution, lateral gene transfer, and as evidence for...Full Text Available

2011-01-01

166

Computational biology for ageing

Development of a fertilized egg into an adult human requires trillions of cell divisions, the vast majority of which duplicate their genome once and only once. Nevertheless, trophoblast giant...Full Text Available

2009-05-15

167

Comparing Genomes within the Species Mycobacterium tuberculosis

High-throughput genomic and proteomic technologies have generated a wealth of publicly available data on ageing. Easy access to these data, and their computational analysis, is of great importance in...Full Text Available

2011-01-12

168

Comparative genomics of insect juvenile hormone biosynthesis?

The study of genetic variability within natural populations of pathogens may provide insight into their evolution and pathogenesis. We used a Mycobacterium tuberculosis high-density...Full Text Available

2001-04-01

169

Ancestral Genomes, Sex, and the Population Structure of Trypanosoma cruzi

The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular...Full Text Available

2006-04-01

170

A framework for evolutionary systems biology

Acquisition of detailed knowledge of the structure and evolution of Trypanosoma cruzi populations is essential for control of Chagas disease....Full Text Available

2006-03-01

171

Infant stepping: a method to study the sensory control of human walking

BackgroundMany difficult problems in evolutionary genomics are related to mutations that have weak effects on fitness, as the consequences of mutations with large effects are often...Full Text Available

172

Using topographic wetness index in vegetation ecology: does the algorithm matter?

Stepping responses were studied in infants between the ages of 10 days and 10 months while they were supported to step on a slowly moving treadmill belt. Surface...Full Text Available

1998-03-15

173

British Library Electronic Table of Contents (United Kingdom)

Abstract Questions: How important is the choice of flow routing algorithm with respect to application of topographic wetness index (TWI) in vegetation ecology? Which flow routing algorithms are preferable for application in vegetation ecology? Location: Forests in three different regions of the Czech Republic. Methods: We used vegetation data from 521 georeferenced plots, recently sampled in a wide range of forest communities. From a digital elevation model, we calculated 11 variations of TWI for each plot with 11 different flow routing algorithms. We evaluated the performance of differently calculated TWI by (1) Spearman rank correlation with average Ellenberg indicator values for soil moisture, (2) Mantel correlation coefficient between dissimilarities of species composition and dissimil...

2010-01-01

174

Spatio-temporal variation of vegetation in an arid and vulnerable coal mining region

British Library Electronic Table of Contents (United Kingdom)

Environmental assessment in an arid coal mining area requires an understanding of the influences of coal mining, the arid climate and ecological remediation. To that end, we selected vegetation as the key environmental factor to observe. Remote sensing approaches to monitoring the spatio-temporal variation of vegetation caused by mining activities, the arid climate and ecological remediation in the Shengdong coal mining area are described. Over a large regional scale it was found that the vegetation was improved as a result of ecological remediation activities. At the local scale, however, the vegetation coverage and soil moisture in the mined areas were slightly lower than those in un-mined areas due to mining subsidence. These differences are partly attributed to ground fissures that inj...

2010-01-01

175

Social-ecological science in the humane metropolis

British Library Electronic Table of Contents (United Kingdom)

The Humane metropolis is a rubric to summarize and promote environmental and social quality in contemporary urban mosaics. Because cities, suburbs, and exurbs, as spatially extensive and connected socio-ecological systems, exhibit many negative features, the humane metropolis identifies a strategy to combat the ills and instill more positive and sustainable features and processes in urban systems. Because the humane metropolis as a program has arisen primarily from social motivations, there is the opportunity to articulate more explicitly the role that science can play in addressing the humane metropolis program and evaluating its success. A humane metropolis can be summarized as one that 1) protects and restores ecological services in cities and suburbs, 2) promotes physical and mental he...

2011-01-01

176

Science and technology for industrial ecology

Ecological risk assessment of water environment for Luanhe River Basin based on relative risk model

Scientific and technological communities have a significant role to play and responsibility for the evolution of global sustainability (continuously improving quality of life into the indefinite future). Sustainability is not possible without a substantially improved science and technology basis for industrial ecology. Society needs data and understanding of complex ecological issues to govern itself in a sustainable manner. We should: support and develop multi-disciplinary programs which create the scientific basis for understanding natural and anthropogenic complex systems and for developing environmentally and economically efficient technology; demonstrate a systems-based approach to science and technology issues which is life-cycle comprehensive, integrates environmental considerations, and promotes conservation of natural resources; and encourage development of responsible, technically and scientifically valid, cost-effective environmental ...

1996-07-10

177

British Library Electronic Table of Contents (United Kingdom)

The relative risk model (RRM) was applied in regional ecological risk assessments successfully. In this study, the RRM was developed through increasing the data of risk source and introducing the source?stressor?habitat exposure filter (SSH), the endpoint?habitat exposure filter (EH) and the stressor?endpoint effect filter (SE) to reflect the meaning of exposure and effect more explicit. Water environment which include water quality, water quantity and aquatic ecosystems was selected as the ecological risk assessment endpoints. The Luanhe River Basin located in the North China was selected as model case. The results showed that there were three low risk regions, one medium risk region and two high risk regions in the Luanhe River Basin. The results also indicated habitat destruction was th...

2010-01-01

178

Climatic change and river ice breakup

Targeted Capture and Next-Generation Sequencing Identifies C9orf75, Encoding Taperin, as the Mutated Gene in Nonsyndromic Deafness DFNB79

An overview of climatic factors and impact relative to river ice engineering and science is presented. An explanation of the fundamentals of climatic change is followed by a review of direct and indirect climatic influences that govern river ice breakup and related trends. Known responses of river ice to climatic change and potential future changes to ice breakup processes are described along with the probable ecological and socio-economic consequences of these changes. Changes in engineering approaches to accommodate the present ice regime and predicted changes in climatic variables that affect river ice processes and reduce the vulnerability of infrastructure and ecosystems to climatic change are examined. Future research on the links between river ice and stream ecology is suggested to identify ecological concerns that may result from changes in river ice regimes induced by climatic change. 60 refs., 3 figs.

2003-07-01

179

Genome-wide association studies of gastric adenocarcinoma and esophageal squamous cell carcinoma identify a shared susceptibility locus in PLCE1 at 10q23

Targeted genome capture combined with next-generation sequencing was used to analyze 2.9 Mb of the DFNB79 interval on chromosome 9q34.3, which includes 108 candidate genes. Genomic...Full Text Available

2010-03-12

180

Draft Genome Sequence of Shewanella sp. Strain HN-41, Which Produces Arsenic-Sulfide Nanotubes.

We conducted a genome-wide association study of gastric cancer (GC) and esophageal squamous cell carcinoma (ESCC) in ethnic Chinese subjects in which we genotyped 551,152 single nucleotide polymorphisms...Full Text Available

2010-09-01

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181

A unique horizontal gene transfer event has provided the octocoral mitochondrial genome with an active mismatch repair gene that has potential for an unusual self-contained function

The dissimilatory metal reducing bacterium Shewanella sp. strain HN-41 was first reported to produce novel photoactive As-S nanotubes via reduction of As(V) and S(2)O(3)(2-) under anaerobic conditions. Here we report the draft genome sequence and annotation of strain HN-41. PMID:21868804

2011-09-01

182

A genome-wide association study of cleft lip with and without cleft palate identifies risk variants near MAFB and ABCA4

BackgroundThe mitochondrial genome of the Octocorallia has several characteristics atypical for metazoans, including a novel gene suggested to function in DNA repair. This mtMutS...Full Text Available

183

Tolerance of Oncomelania hupensis quadrasi to varying concentrations of dissolved oxygen and organic pollution*

Case-parent trios were used in a genome wide association study of cleft lip with/without cleft palate (CL/P). SNPs near two genes not previously associated with CL/P [MAFB:...Full Text Available

2010-06-01

184

The ecology of the coastal marshes of western Lake Erie: A community profile

Ecological investigations were made of habitats containing natural populations of the snail Oncomelania hupensis quadrasi and of habitats free from the snail in the island of Leyte,...Full Text Available

1972-01-01

185

The Charles University in Prague Environment Centre - Environmental Kuznets curve

Lake Erie, the southernmost of the Laurentian Great Lakes, is narrow and relatively shallow in comparison to other Great Lakes. The lake experiences extreme water level fluctuations and storm energy restricts the development of wetlands to protected areas within embayments, lagoons, or behind barriers. However, coastal marshes of western Lake Erie fringe the shorelines of Michigan, Ohio, and Ontario and encompass an area of 268 km/sup 2/. This publication reviews the ecological data and information on the wetlands of Lake Erie, which are some of the more productive areas in the Great Lakes ecosystem. The geologic history of the Lake Erie leading to the development of wetlands, the present environment, and present wetland distribution are presented as background in the opening chapters. Biological information available for the Lake Erie wetlands is discussed in detail, and ecological processes contributing to the evolution of wetlands, ...

1987-02-01

186

Sustainable development in city districts: BaLaLuZ project - Building ecology; Schlussbericht 'Gebaeudeoekologie' - Phase 1

... The main goal was the analysis of the Environmental Kuznets Curve hypothesis for the Czech Republic. A paper by Bruha and Scasny, accepted to the European Society of Ecological Economics, ( Lisbon June 2005), analyses driving forces (including economic policy) on ...

187

Stable isotopes document the trophic structure of a deep-sea cephalopod assemblage including giant octopod and giant squid

This final report for the Swiss Federal Office of Energy (SFOE) is one of a series of reports concerning municipal development in various cities in Switzerland. The four city districts involved include Basel (Gundeldinger Feld), Lausanne (Bellevaux), Lucerne (Basel-/Bernstrasse) and Zurich (Werdwies). This paper takes a look at aspects of building ecology. In the four areas, the following building types and projects were examined with respect to their ecology: Basel: conversion of commercial premises to a community centre, Lausanne and Lucerne: Enhancement of residential areas, Zurich: a new residential building. Criteria examined include general building ecology, building materials, raw materials, toxic substances, recycling, maintenance and deconstruction, energy for heating and hot water, grey energy, electricity, ground usage, water, wastes and public infrastructure. Knowledge gained along with questions and problems ...

2004-07-01

188

Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica

Although deep-sea cephalopods are key marine organims, their feeding ecology remains essentially unknown. Here, we report for the first time the trophic structure of an assemblage of these animals (19...Full Text Available

2009-06-23

189

Scaling of offspring number and mass to plant and animal size: model and meta-analysis

A previous report of high levels of members of the domain Archaea in Antarctic coastal waters prompted us to investigate the ecology of Antarctic planktonic prokaryotes. rRNA hybridization...Full Text Available

1998-07-01

190

Road ecology from a road-side assemblage of forest birds in south-western Australia

The scaling of reproductive parameters to body size is important for understanding ecological and evolutionary patterns. Here, we derived allometric relationships for the number and mass of seeds, eggs...Full Text Available

2008-04-01

191

Red tides in the Gulf of Mexico: Where, when, and why?

... verges of a two-lane highway in continuous Jarrah Eucalyptus marginata forest of south-western Australia. Midway during this ... Birds were recorded from the beginning of continuous Jarrah Eucalyptus marg...

192

Rainforest Portal Rainforest Conservation Links: Information/Maps

[1] Independent data from the Gulf of Mexico are used to develop and test the hypothesis that the same sequence of physical and ecological events each year allows the toxic dinoflagellate Karenia...Full Text Available

2006-11-07

193

Mortality, Recruitment and Change of Desert Tree Populations in a Hyper-Arid Environment

... 12, 2007 | Rate It Distribution and Variety of Equatorial Rain Forest , The http://www.esd.ornl.gov/projects/qen/rainfo.html (3 votes) an in depth examination of ecological patterns in rainforests from the Oak Ridge National Laboratory Added: Mar. 17, 2001 ...

194

Molecular Ecology of Pyrethroid Knockdown Resistance in Culex pipiens pallens Mosquitoes

BackgroundLong-term vegetation changes in hyper-arid areas have long been neglected. Mortality, recruitment and change in populations of the ecologically and culturally important...Full Text Available

195

Lizards in the ecology of salmonellosis in Panama.

Pyrethroid insecticides have been extensively used in China and worldwide for public health pest control. Accurate resistance monitoring is essential to guide the rational use of insecticides and resistance...Full Text Available

196

Life on the edge: carnivore body size variation is all over the place

Enteropathogenic bacteria was isolated from 131 of 447 (29.4%) neotropical Panamanian lizards belonging to 34 species of seven families. Overall, 147 strains of bacteria were isolated comprising 26...Full Text Available

1981-05-01

197

Invasion and control of water hyacinth (Eichhornia crassipes) in China*

Evolutionary biologists have long been fascinated by both the ways in which species respond to ecological conditions at the edges of their geographic ranges and the way that species' body sizes evolve...Full Text Available

2009-04-22

198

Influence of a Salinity Gradient on the Vessel Characters of the Mangrove Species Rhizophora mucronata

By the time of primary 21st century, water hyacinth had become a serious environmental problem in China. Water hyacinth contributes to the major part of ecological hazards from the invasion of foreign...Full Text Available

2006-08-01

199

In vitro digestibility of fern and gymnosperm foliage: implications for sauropod feeding ecology and diet selection

• Background and Aims Although mangroves have been extensively studied, little is known about their ecological wood anatomy. This investigation examined the potential use of...Full Text Available

2006-12-01

200

Estimation of effective population sizes from data on genetic markers

Sauropod dinosaurs, the dominant herbivores throughout the Jurassic, challenge general rules of large vertebrate herbivory. With body weights surpassing those of any other megaherbivore, they relied...Full Text Available

2008-05-07

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201

Effect of Wastewater Treatment Plant Effluent on Microbial Function and Community Structure in the Sediment of a Freshwater Stream with Variable Seasonal Flow?

The effective population size (N_e) is an important parameter in ecology, evolutionary biology and conservation biology. It is, however, notoriously difficult to estimate,...Full Text Available

2005-07-29

202

Ecological relevance of air pollution abatement measures as a decision aid

We investigated the effects of wastewater treatment plant (WWTP) discharge on the ecology of bacterial communities in the sediment of a small, low-gradient stream in South Australia. The quantification...Full Text Available

2008-05-01

203

Dorsal cortex volume in male side-blotched lizards (Uta stansburiana) is associated with different space use strategies

Air pollution abatement measures are not an end in themselves, and undesirable side effects should be taken into account. The proposed emission index takes into account the total emissions caused by a technical project as well as the effects of the individual pollutants.

1983-01-01

204

Dissecting the Genetic Components of Adaptation of Escherichia coli to the Mouse Gut

Spatial abilities have been associated with many ecologically-relevant behaviors such as territoriality, mate choice, navigation and acquisition of food resources. Differential demands on spatial...Full Text Available

2009-07-01

205

Cytogenetic analysis of three sea catfish species (Teleostei, Siluriformes, Ariidae) with the first report of Ag-NOR in this fish family

While pleiotropic adaptive mutations are thought to be central for evolution, little is known on the downstream molecular effects allowing adaptation to complex ecologically relevant environments. Here...Full Text Available

2008-01-01

206

Collective trauma in northern Sri Lanka: a qualitative psychosocial-ecological study

Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been...Full Text Available

2010-04-01

207

Clouded leopards, the secretive top-carnivore of South-East Asian rainforests: their distribution, status and conservation needs in Sabah, Malaysia

BackgroundComplex situations that follow war and natural disasters have a psychosocial impact on not only the individual but also on the family, community and society. Just as the...Full Text Available

208

Analysis of Quantitative Interactions between Two Species of Arbuscular Mycorrhizal Fungi, Glomus mosseae and G. intraradices, by Real-Time PCR

BackgroundThe continued depletion of tropical rainforests and fragmentation of natural habitats has led to significant ecological changes which place most top carnivores under heavy...Full Text Available

209

Ammonium and Nitrate Uptake by the Floating Plant Landoltia punctata

Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs, known to play an important role in ecological processes. Conventional light microscopy is the most common method used to detect their presence...Full Text Available

2006-06-01

210

A spatial optimization of biodiversity and timber values in development of an integrated conservation area design in Southeast Alaska

Background and AimsPlants from the family Lemnaceae are widely used in ecological engineering projects to purify wastewater and eutrophic water bodies. However, the biology of nutrient...Full Text Available

2007-02-01

211

... relative suitability of areas for production of commercial timber was determined based on considerations of operability, proximity ... habitats, and an ecologically-based estimate of sustainable timber pr...

212

27na4

Complete genome sequence of Actinosynnema mirum type strain (101T)

by American scientists, ecosystems containing a greater number of plant species, produce more biomass.This result ...to meet the increasing demand for land for farmland planted with monocultures, buildings and roads.For ...have hypothesized that greater ecological diversity (diversity of plant and animal species) leads to a greater

213

Bisulfite genomic sequencing of DNA from dried blood spot microvolume samples.

Actinosynnema mirum Hasegawa et al. 1978 is the type species of the genus, and is of phylogenetic interest because of its central phylogenetic location in the Actino-synnemataceae, a rapidly growing family within the actinobacterial suborder Pseudo-nocardineae. A. mirum is characterized by its motile spores borne on synnemata and as a producer of nocardicin antibiotics. It is capable of growing aerobically and under a moderate CO2 atmosphere. The strain is a Gram-positive, aerial and substrate mycelium producing bacterium, originally isolated from a grass blade collected from the Raritan River, New Jersey. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first complete genome sequence of a member of the family Actinosynnemataceae, and only the second sequence from the actinobacterial suborder Pseudonocardineae. The 8,248,144 bp long single replicon genome ...

2009-05-20

214

Spatial heterogeneity and ecological models. [Predation

DNA methylation is an important event in epigenetic changes in cells, and a fundamental regulator of gene transcription. Bisulfite genomic sequencing is a powerful technique used in studies of DNA methylation. However, the established procedures often require relatively large amounts of DNA. In everyday practice, samples submitted for analysis might contain very small amounts of poor quality material, as is often the case with forensic stain samples. In this study, we assess a modified, more efficient method of bisulfite genomic sequencing. Genomic DNA extracted from 3-mm dried blood spots using QIAamp micro kit was treated with sodium bisulfite (using EpiTect kit). Subsequent methylation-specific PCR (MSP) followed by DNA sequencing displayed the differentially methylated region of imprinted gene SNRPN. Our results show that this new combination of efficient DNA extraction and bisulfite treatment provides high quality ...

2011-07-01

215

On the optimal taxation of common-pool resources

The role of natural enemies in the regulation of populations is one of the major questions facing population ecologists. Simplification have led to two theoretical ways of incorporating the role of natural enemies in single ecological models: diffusion models and patch-type models. The predictions of the models are different because of the way variability is incorporated. Three equations are presented for diffusion models and one for patch models. Since the two types of models apply at different combinations of spatial and temporal scales, the right model(s) to choose for a particular study requires careful assessment. A continuing dialogue between experimentalists and theoreticians will lead to a better understanding of natural systems such as those that occur in biological control.

1990-04-01

216

British Library Electronic Table of Contents (United Kingdom)

Recent research developments in common-pool resource models emphasize the importance of links with ecological systems and the presence of non-linearities, thresholds and multiple steady states. In a recent paper Kossioris et al. (2008) develop a methodology for deriving feedback Nash equilibria for non-linear differential games and apply this methodology to a common-pool resource model of a lake where pollution corresponds to benefits and at the same time affects the ecosystem services. This paper studies the structure of optimal state-dependent taxes that steer the combined economic-ecological system towards the trajectory of optimal management, and provides an algorithm for calculating such taxes.

2011-01-01

217

Ecosystem restoration versus reclamation: the value of managing for biodiversity

First-principles investigation of Co wires at Pt(111) step-edges

The importance of ecosystem restoration to land reclamation is discussed. If a plant community is to be self sustaining, natural diversity processes provide a useful model for restoration. Biodiversity is important for utilitarian, ecological, and ethical/aesthetic reasons. Issues that must be considered include sampling and management scales, the separate richness of the diverse array of species playing a role in ecosystem productivity and stability, and natural trends in diversity. Ecological restoration comprises two fundamental activities: management for some pre-disturbance level of biodiversity and the combination of species in a manner that is essentially experimental. Endeavours to restore biological diversity are a useful method of experimenting with the factors that control ecosystem structure and function. 21 refs.

1991-06-01

218

Kootenai River Floodplain Ecosystem Operational Loss Assessment, Protection, Mitigation and Rehabilitation, 2007-2008 Annual Report.

No abstract prepared.

2006-07-01

219

Nitrogen oxides decreasing combustion method

The overarching goals of the 'Kootenai River Floodplain Ecosystem Operational Loss Assessment, Protection, Mitigation and Rehabilitation' Project (BPA Project No.2002-011-00) are to: (1) assess abiotic and biotic factors (i.e., geomorphologic, hydrological, aquatic and riparian/floodplain communities) in determining a definitive composition of ecological integrity, (2) develop strategies to assess and mitigate losses of ecosystem functions, and (3) produce a regional operational loss assessment framework. To produce a scientifically defensible, repeatable, and complete assessment tool, KTOI assembled a team of top scientists in the fields of hydrology, hydraulics, ornithology, entomology, statistics, and river ecology, among other expertise. This advisory team is known as the Research Design and Review Team (RDRT). The RDRT scientists drive the review, selection, and adaptive management of the research designs to evaluate the ...

2009-02-18

220

Trans-generational radiation-induced chromosomal instability in the female enhances the action of chemical mutagens

This patent describes a nitrogen oxides decreasing combustion method which comprises: a first step of mixing a fuel and air with each other; a second step of bringing the mixture obtained in the first step into contact with a packed catalyst such that only catalytic combustion occurs; and a third step of adding a fresh supply of the fuel to a stream obtained from the second step to form a mixed gas and causing the mixed gas to undergo non-catalytic thermal combustion. The temperature of the packed catalyst is lower than the ignition temperature of the mixture and the adiabatic flame temperature of the mixed gas is lower than a temperature at which the nitrogen oxides occur.

1988-03-22

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221

Exploiting rice-sorghum synteny for targeted development of EST-SSRs to enrich the sorghum genetic linkage map.

Genomic instability can be produced by ionising radiation, so-called radiation-induced genomic instability, and chemical mutagens. Radiation-induced genomic instability occurs in both germinal and somatic cells and also in the offspring of irradiated individuals, and it is characterised by genetic changes including chromosomal rearrangements. The majority of studies of trans-generational, radiation-induced genomic instability have been described in the male germ line, whereas the authors who have chosen the female as a model are scarce. The aim of this work is to find out the radiation-induced effects in the foetal offspring of X-ray-treated female rats and, at the same time, the possible impact of this radiation-induced genomic instability on the action of a chemical mutagen. In order to achieve both goals, the quantity and quality of chromosomal damage were analysed. In order to ...

2008-04-02

222

SEPT9_i1 and genomic instability: Mechanistic insights and relevance to tumorigenesis

The sequencing and detailed comparative functional analysis of genomes of a number of select botanical models open new doors into comparative genomics among the angiosperms, with potential benefits for improvement of many orphan crops that feed large populations. In this study, a set of simple sequence repeat (SSR) markers was developed by mining the expressed sequence tag (EST) database of sorghum. Among the SSR-containing sequences, only those sharing considerable homology with rice genomic sequences across the lengths of the 12 rice chromosomes were selected. Thus, 600 SSR-containing sorghum EST sequences (50 homologous sequences on each of the 12 rice chromosomes) were selected, with the intention of providing coverage for corresponding homologous regions of the sorghum genome. Primer pairs were designed and polymorphism detection ability was assessed using parental pairs of two existing sorghum ...

2009-08-08

223

British Library Electronic Table of Contents (United Kingdom)

Abstract Septins are highly conserved cytoskeletal GTP-binding proteins implicated in numerous cellular processes from apoptosis to vesicle trafficking. Septins have been associated with leukemia and solid tumor malignancies, including breast, ovarian, and prostate. We previously reported that high SEPT9_i1 expression in human mammary epithelial cell lines (HMECs) led to malignant cellular phenotypes such as increased cell proliferation, invasiveness, motility, and genomic instability. Our goal here was to better understand how SEPT9_i1 expression might contribute to genomic instability and malignant progression. First, we confirmed that even transient expression of SEPT9_i1 was sufficient to increase aneuploidy in HMECs. We then analyzed SEPT9_i1 by immunoprecipitation and immunofluoresce...

2011-01-01

224

Federal technology transfer and the human genome project. Background paper

A genomic library-based amplification approach (GL-PCR) for the mapping of multiple IS6110 insertion sites and strain differentiation of Mycobacterium tuberculosis

As with other areas of biomedical research, the expectation is that the results of genome research will yield commercially valuable products of benefits to human health. The report, analyzes universities`, companies`, and researchers` experiences and perspectives since enactment of federal laws to enhance technology transfer--especially as it pertains to research funded by the National Institutes of Health and the Department of Energy, the agencies funding U.S. efforts in the Human Genome Project. OTA prepared this background paper with the assistance of a panel of advisors and reviewers selected for their expertise and diverse points of view. Additionally, hundreds of individuals cooperated with OTA staff through interviews or by providing written material. These authorities were drawn from government, academia, industry, and professional societies worldwide.

1995-09-01

225

British Library Electronic Table of Contents (United Kingdom)

Evidence suggests that insertion of the IS6110 element is not without consequence to the biology of Mycobacterium tuberculosis complex strains. Thus, mapping of multiple IS6110 insertion sites in the genome of biomedically relevant clinical isolates would result in a better understanding of the role of this mobile element, particularly with regard to transmission, adaptability and virulence. In the present paper, we describe a versatile strategy, referred to as GL-PCR, that amplifies IS6110-flanking sequences based on the construction of a genomic library. M. tuberculosis chromosomal DNA is fully digested with HincII and then ligated into a plasmid vector between T7 and T3 promoter sequences. The ligation reaction product is transformed into Escherichia coli and selective PCR amplification...

2006-01-01

226

solQTL: a tool for QTL analysis, visualization and linking to genomes at SGN database

piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells

BackgroundA common approach to understanding the genetic basis of complex traits is through identification of associated quantitative trait loci (QTL). Fine mapping QTLs requires...Full Text Available

227

chipD: a web tool to design oligonucleotide probes for high-density tiling arrays

A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration...Full Text Available

2006-10-10

228

Visual Genome-Wide RNAi Screening to Identify Human Host Factors Required for Trypanosoma cruzi Infection

chipD is a web server that facilitates design of DNA oligonucleotide probes for high-density tiling arrays, which can be used in a number of genomic applications such as ChIP-chip or gene-expression...Full Text Available

2010-07-01

229

Viruses with More Than 1,000 Genes: Mamavirus, a New Acanthamoeba polyphaga mimivirus Strain, and Reannotation of Mimivirus Genes

The protozoan parasite Trypanosoma cruzi is the etiologic agent of Chagas disease, a neglected tropical infection that affects millions of people in the Americas. Current chemotherapy...Full Text Available

230

Ubiquitin over-expression phenotypes and ubiquitin gene molecular misreading during aging in Drosophila melanogaster

The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus...Full Text Available

2011-01-01

231

Two separate domains within vesicular stomatitis virus phosphoprotein support transcription when added in trans.

Molecular Misreading (MM) is the inaccurate conversion of genomic information into aberrant proteins. For example, when RNA polymerase II transcribes a GAGAG motif it synthesizes at low frequency RNA...Full Text Available

232

Tumour suppressor ING1b maintains genomic stability upon replication stress

The structural phosphoprotein NS of vesicular stomatitis virus, in association with the virion-associated RNA polymerase L protein, transcribes the genome ribonucleoprotein template in vitro. It contains...Full Text Available

1987-12-01

233

Tumor-Endothelial Interaction Links the CD44⁺/CD24^- Phenotype with Poor Prognosis in Early-Stage Breast Cancer1

The lesion bypass pathway, which is regulated by monoubiquitination of proliferating cell nuclear antigen (PCNA), is essential for resolving replication stalling due to DNA lesions. This process is...Full Text Available

2011-05-01

234

Transposons as tools for enhancer trap screens in vertebrates

Materials and MethodsThe genomic effects of tumor-endothelial interactions in cancer are not yet well characterized. To study this interaction in breast...Full Text Available

2009-10-01

235

Transposon-based screens for cancer gene discovery in mouse models

DNA transposons are efficient tools in transgenesis and have therefore become popular in the analysis of the regulatory genome in vertebrates via enhancer trap screens. Here, I discuss recent progress...Full Text Available

2007-01-01

236

Transposable elements in fish functional genomics: technical challenges and perspectives

Significant emphasis has recently been placed on the characterization of the human cancer genome. This effort has been assisted by the development of new DNA sequencing technologies that allow...Full Text Available

2010-08-01

237

Transposable elements are enriched within or in close proximity to xenobiotic-metabolizing cytochrome P450 genes

The recent introduction of several transposable elements in zebrafish opens new frontiers for genetic manipulation in this important vertebrate model. This review discusses transposable elements as...Full Text Available

2007-01-01

238

Transcriptional Organization of the Avian Adenovirus CELO

BackgroundTransposons, i.e. transposable elements (TEs), are the major internal spontaneous mutation agents for the variability of eukaryotic genomes. To address the general issue...Full Text Available

239

Tissue Effect on Genetic Control of Transcript Isoform Variation

A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs)....Full Text Available

1998-11-01

240

The struggle for life of the genome's selfish architects

Current genome-wide association studies (GWAS) are moving towards the use of large cohorts of primary cell lines to study a disease of interest and to assign biological relevance to the genetic signals...Full Text Available

2009-08-01

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241

The polymorphism architecture of mouse genetic resources elucidated using genome-wide resequencing data: implications for QTL discovery and systems genetics

Transposable elements (TEs) were first discovered more than 50 years ago, but were totally ignored for a long time. Over the last few decades they have gradually attracted increasing interest from research...Full Text Available

242

The missing graphical user interface for genomics

Mouse genetic resources include inbred strains, recombinant inbred lines, chromosome substitution strains, heterogeneous stocks, and the Collaborative Cross (CC). These resources were generated through...Full Text Available

2007-07-01

243

The complete mitochondrial genome of Atelura formicaria (Hexapoda: Zygentoma) and the phylogenetic relationships of basal insects.

AbstractThe Galaxy package empowers regular users to perform rich DNA sequence analysis through a much-needed and user-friendly graphical web interface.See research article...Full Text Available

2010-01-01

244

The chromosomal association/dissociation of the chromatin insulator protein Cp190 of Drosophila melanogaster is mediated by the BTB/POZ domain and two acidic regions

In this study, the complete sequence of the mitochondrial DNA (mtDNA) of Atelura formicaria (Hexapoda: Zygentoma) is described. The molecule is 15.205 bp in length and it is the third complete mt genome sequenced from the Zygentoma. The genome organization conforms with the putative ancestral insect gene arrangement. All protein coding genes use standard initiation codons (methionine and isoleucine). The exception is nad4 that starts with GTG, a codon used for this purpose in other insect species. A peculiar strand skew bias is observed, given that the PCGs encoded on the J-strand contain more thymines than adenines and more cytosines than guanines. This trend in nucleotide composition has been observed also in the "firebrat" Thermobia domestica (Zygentoma, Lepismatidae), but differs from that of the majority of hexapod species, including Tricholepidion gertschi (Zygentoma, Lepidotrichidae), where adenines and cytosines outnumber thymines and ...

2009-03-11

245

The Pseudomonas aeruginosa PA01 Gene Collection

BackgroundChromatin insulators or boundary elements are a class of functional elements in the eukaryotic genome. They regulate gene transcription by interfering with promoter-enhancer...Full Text Available

246

The Human Ageing Genomic Resources: online databases and tools for biogerontologists

Pseudomonas aeruginosa, a common inhabitant of soil and water, is an opportunistic pathogen of growing clinical relevance. Its genome, one of the largest among bacteria [5570 open reading...Full Text Available

2004-10-01

247

The Exceptionally Large Genome of Hendra Virus: Support for Creation of a New Genus within the Family Paramyxoviridae

SummaryAgeing is a complex, challenging phenomenon that will require multiple, interdisciplinary approaches to unravel its puzzles. To assist basic research on ageing, we developed...Full Text Available

2009-02-01

248

The Anopheles gambiae glutathione transferase supergene family: annotation, phylogeny and expression profiles

An outbreak of acute respiratory disease in Hendra, a suburb of Brisbane, Australia, in September 1994 resulted in the deaths of 14 racing horses and a horse trainer. The causative agent was a new member...Full Text Available

2000-11-01

249

Ternary Complex Formation on the Adenovirus Packaging Sequence by the IVa2 and L4 22-Kilodalton Proteins?

BackgroundTwenty-eight genes putatively encoding cytosolic glutathione transferases have been identified in the Anopheles gambiae genome. We manually annotated these...Full Text Available

250

Studying bacterial transcriptomes using RNA-seq

Assembly of infectious adenovirus particles requires seven functionally redundant elements at the left end of the genome, termed A repeats, that direct packaging of the DNA. Previous studies revealed...Full Text Available

2007-11-01

251

Structural analysis of a hepatitis B virus genome integrated into chromosome 17p of a human hepatocellular carcinoma.

Genome-wide studies of bacterial gene expression are shifting from microarray technology to second generation sequencing platforms. RNA-seq has a number of advantages over hybridization-based techniques,...Full Text Available

2010-10-01

252

Structural Basis for Acetylated Histone H4 Recognition by the Human BRD2 Bromodomain*

Hepatitis B virus (HBV) is clearly a factor in the development of hepatocellular carcinoma, but its mechanism of action remains obscure. One possibility is that the HBV integration event alters the...Full Text Available

1988-11-01

253

Statistical properties of nucleotide clusters in DNA sequences*

Recognition of acetylated chromatin by the bromodomains and extra-terminal domain (BET) family proteins is a hallmark for transcriptional activation and anchoring viral genomes to mitotic chromosomes...Full Text Available

2010-03-05

254

Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus

Using the complete genome of Plasmodium falciparum 3D7 which has 14 chromosomes as an example, we have examined the distribution functions for the amount of C or G and A or T consecutively...Full Text Available

2005-05-01

255

Severe teratozoospermia and its influence on pronuclear morphology, embryonic cleavage and compaction

BackgroundRNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome...Full Text Available

2010-01-01

256

Restriction endonuclease analysis of porcine Pasteurella multocida isolates from Quebec.

BackgroundFertilization, cell division and embryo development depend on genomic contributions from male and female gametes. We hypothesize that teratozoospermic sperm influences...Full Text Available

257

Resolving Individuals Contributing Trace Amounts of DNA to Highly Complex Mixtures Using High-Density SNP Genotyping Microarrays

We have used restriction endonuclease analysis (REA) of genomic DNA to classify porcine Pasteurella multocida isolates with similar capsular and somatic serotypes, and to monitor the distribution of...Full Text Available

1990-10-01

258

ReplicationDomain: a visualization tool and comparative database for genome-wide replication timing data

We use high-density single nucleotide polymorphism (SNP) genotyping microarrays to demonstrate the ability to accurately and robustly determine whether individuals are in a complex genomic DNA mixture....Full Text Available

2008-08-01

259

Remobilization of Tol2 transposons in Xenopus tropicalis

BackgroundEukaryotic DNA replication is regulated at the level of large chromosomal domains (0.5–5 megabases in mammals) within which replicons are activated relatively synchronously....Full Text Available

260

Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups

BackgroundThe Class II DNA transposons are mobile genetic elements that move DNA sequence from one position in the genome to another. We have previously demonstrated that the naturally...Full Text Available

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261

Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication

The evolution of the human mitochondrial genome is characterized by the emergence of ethnically distinct lineages or haplogroups. Nine European, seven Asian (including Native American), and three African...Full Text Available

2002-05-01

262

Polymorphic Regions Affecting Human Height Also Control Stature in Cattle

Human cytomegalovirus (HCMV) in clinical material cannot replicate efficiently in vitro until it has adapted by mutation. Consequently, wild-type HCMV differ fundamentally from the passaged strains...Full Text Available

2010-09-01

263

Palindromic Sequence Plays a Critical Role in Human Foamy Virus Dimerization

Orthologous positions of 55 genes associated with height in four human populations were located on the bovine genome. Single nucleotide polymorphisms close to eight of these genes were significantly...Full Text Available

2011-03-01

264

Onset of Quiescence Following p53 Mediated Down-Regulation of H2AX in Normal Cells

The retroviral RNA genome is dimeric, consisting of two identical strands of RNA linked near their 5′ ends by a dimer linkage structure. Previously it was shown that human foamy virus (HFV)...Full Text Available

2001-04-01

265

New common variants affecting susceptibility to basal cell carcinoma

Normal cells, both in vivo and in vitro, become quiescent after serial cell proliferation. During this process, cells can develop immortality with genomic instability,...Full Text Available

266

New Pathogenicity Marker Found in the Plasticity Region of the Helicobacter pylori Genome

In a follow-up to our previously reported genome-wide association study of cutaneous basal cell carcinoma (BCC)1, we describe here several new susceptibility...Full Text Available

2009-08-01

267

Mutations in TPRN Cause a Progressive Form of Autosomal-Recessive Nonsyndromic Hearing Loss

Comparison of gastric carcinoma and gastritis isolates showed the presence of genes, probably carcinoma associated (JHP947 and JHP940), that are situated in a Helicobacter...Full Text Available

2003-04-01

268

Mutational Analysis of cis-Acting RNA Signals in Segment 7 of Influenza A Virus?

We performed genome-wide homozygosity mapping in a large consanguineous family from Morocco and mapped the autosomal-recessive nonsyndromic hearing loss (ARNSHL) in this family to the DFNB79...Full Text Available

2010-03-12

269

Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

The genomic viral RNA (vRNA) segments of influenza A virus contain specific packaging signals at their termini that overlap the coding regions. To further characterize cis-acting signals...Full Text Available

2008-12-01

270

Mapping cis-Regulatory Domains in the Human Genome UsingMulti-Species Conservation of Synteny

BackgroundFor more than a decade there has been increasing interest in the use of nanotechnology and microarray platforms for diagnostic applications. In this report, we describe...Full Text Available

271

Longitudinal assessment of lung cancer progression in the mouse using in vivo micro-CT imaging

Our inability to associate distant regulatory elements with the genes that they regulate has largely precluded their examination for sequence alterations contributing to human disease. One major obstacle is the large genomic space surrounding targeted genes in which such elements could potentially reside. In order to delineate gene regulatory boundaries we used whole-genome human-mouse-chicken (HMC) and human-mouse-frog (HMF) multiple alignments to compile conserved blocks of synteny (CBS), under the hypothesis that these blocks have been kept intact throughout evolution at least in part by the requirement of regulatory elements to stay linked to the genes that they regulate. A total of 2,116 and 1,942 CBS>200 kb were assembled for HMC and HMF respectively, encompassing 1.53 and 0.86 Gb of human sequence. To support the existence of complex long-range regulatory domains within these CBS we analyzed the prevalence and distribution of ...

2005-06-13

272

Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies

Purpose: Small animal micro-CT imaging is being used increasingly in preclinical biomedical research to provide phenotypic descriptions of genomic models. Most of this imaging is coincident...Full Text Available

2010-09-01

273

Kinetic Complexity of the Global Response to Glucocorticoid Receptor Action

Recently, genome-wide association studies (GWAS) have linked the human LIN28B locus to height and timing of menarche [1-Full Text Available

2010-07-01

274

Interpopulation hybridization results in widespread viability selection across the genome in Tigriopus californicus

We have characterized the kinetic response of gene targets throughout the murine genome to transcriptional modulation by the glucocorticoid receptor (GR). In contrast to a model in which multiple genes...Full Text Available

2009-04-01

275

Integrase-directed recovery of functional genes from genomic libraries

BackgroundGenetic interactions within hybrids influence their overall fitness. Understanding the details of these interactions can improve our understanding of speciation. One experimental...Full Text Available

276

Insights into the Genome of Large Sulfur Bacteria Revealed by Analysis of Single Filaments

Large population sizes, rapid growth and 3.8 billion years of evolution firmly establish microorganisms as a major source of the planet's biological and genetic diversity. However, up to 99% of the...Full Text Available

2009-09-01

277

Inferring transcription factor complexes from ChIP-seq data

Marine sediments are frequently covered by mats of the filamentous Beggiatoa and other large nitrate-storing bacteria that oxidize hydrogen sulfide using either oxygen or nitrate, which...Full Text Available

2007-09-01

278

In vitro dimerization of human immunodeficiency virus type 1 (HIV-1) spliced RNAs

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) allows researchers to determine the genome-wide binding locations of individual transcription factors (TFs) at high resolution....Full Text Available

2011-08-01

279

Impaired replication dynamics at the FRA3B common fragile site

The human immunodeficiency virus type 1 (HIV-1) packages its genomic RNA as a dimer of homologous RNA molecules that has to be selected among a multitude of cellular and viral RNAs. Interestingly, spliced...Full Text Available

2007-12-01

280

Identification of plasmid and Bacillus subtilis chromosomal recombination sites used for pE194 integration.

Chromosomal common fragile sites (CFSs) are genetically unstable regions of the genome that are induced by conditions that impair DNA replication. In this report, we show that treatment with the DNA...Full Text Available

2010-01-01

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281

Identification of genomic differences between Campylobacter jejuni subsp. jejuni and C. jejuni subsp. doylei at the nap locus leads to the development of a C. jejuni subspeciation multiplex PCR method

The plasmid pE194 (3.7 kilobases) is capable of integrating into the genome of the bacterial host Bacillus subtilis in the absence of the major homology-dependent RecE recombination system. Multiple...Full Text Available

1989-05-01

282

Identification of copy number variations and common deletion polymorphisms in cattle

BackgroundThe human bacterial pathogen Campylobacter jejuni contains two subspecies: C. jejuni subsp. jejuni (Cjj)...Full Text Available

283

Identification of a Central DNA Flap in Feline Immunodeficiency Virus

BackgroundRecently, the discovery of copy number variation (CNV) led researchers to think that there are more variations of genomic DNA than initially believed. Moreover, a certain...Full Text Available

284

Identification of Genes Affecting the Toxicity of Anti-Cancer Drug Bortezomib by Genome-Wide Screening in S. pombe

A duplication of the polypurine tract (PPT) at the center of the human immunodeficiency virus type 1 (HIV-1) genome (the cPPT) has been shown to prime a separate plus-strand initiation and to result...Full Text Available

2001-10-01

285

Identification and In Vivo Characterization of NvFP-7R, a Developmentally Regulated Red Fluorescent Protein of Nematostella vectensis

Bortezomib/PS-341/Velcade, a proteasome inhibitor, is widely used to treat multiple myeloma. While several mechanisms of the cytotoxicity of the drug were proposed, the actual mechanism remains elusive....Full Text Available

286

Human Immunodeficiency Virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells

BackgroundIn recent years, the sea anemone Nematostella vectensis has emerged as a critical model organism for comparative genomics and developmental biology. Although...Full Text Available

287

Genomic patterns of pathogen evolution revealed by comparison of Burkholderia pseudomallei, the causative agent of melioidosis, to avirulent Burkholderia thailandensis

BackgroundHIV-1 reverse transcriptase (RT) is a heterodimer composed of p66 and p51 subunits and is responsible for reverse transcription of the viral RNA genome into DNA. RT can...Full Text Available

288

Genomic but Not Antigenomic Hepatitis Delta Virus RNA Is Preferentially Exported from the Nucleus Immediately after Synthesis and Processing

BackgroundThe Gram-negative bacterium Burkholderia pseudomallei (Bp) is the causative agent of the human disease melioidosis. To understand the evolutionary mechanisms...Full Text Available

289

Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent

Hepatitis delta virus (HDV) contains a viroid-like circular RNA that replicates via a double rolling circle replication mechanism. It is generally assumed that HDV RNA is synthesized and remains exclusively...Full Text Available

2002-04-01

290

Genomic Expression Libraries for the Identification of Cross-Reactive Orthopoxvirus Antigens

Harmful algal blooms, caused by rapid growth and accumulation of certain microalgae in the ocean, pose considerable impacts on marine environments, aquatic industries and even public health. Here, we...Full Text Available

2005-01-01

291

Genomic Evidence for the Evolution of Streptococcus equi: Host Restriction, Increased Virulence, and Genetic Exchange with Human Pathogens

Increasing numbers of human cowpox virus infections that are being observed and that particularly affect young non-vaccinated persons have renewed interest in this zoonotic disease. Usually causing...Full Text Available

292

Genomic Diversity and Evolution of Mycobacterium ulcerans Revealed by Next-Generation Sequencing

The continued evolution of bacterial pathogens has major implications for both human and animal disease, but the exchange of genetic material between host-restricted pathogens is rarely considered....Full Text Available

2009-03-01

293

Genomic Content of Bordetella pertussis Clinical Isolates Circulating in Areas of Intensive Children Vaccination

Mycobacterium ulcerans is the causative agent of Buruli ulcer, the third most common mycobacterial disease after tuberculosis and leprosy. It is an emerging infectious disease that...Full Text Available

2009-09-01

294

Genome-wide profiling of forum domains in Drosophila melanogaster

BackgroundThe objective of the study was to analyse the evolution of Bordetella pertussis population and the influence of herd immunity in different areas of the...Full Text Available

295

Genome-wide linkage scan for contraction velocity characteristics of knee musculature in the Leuven Genes for Muscular Strength Study

Forum domains are stretches of chromosomal DNA that are excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Most forum domains are 50–200 kb in length....Full Text Available

2011-05-01

296

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

The torque-velocity relationship is known to be affected by ageing, decreasing its protective role in the prevention of falls. Interindividual variability in this torque-velocity relationship is partly...Full Text Available

2008-09-01

297

Genome-Wide Transcriptome Profiling of Region-Specific Vulnerability to Oxidative Stress in the Hippocampus

Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their in vivo counterparts in many aspects and exhibit a higher proportion of developmental...Full Text Available

2008-12-16

298

Genome-Wide Transcriptional Response of Chemostat-Cultured Escherichia coli to Zinc

Neurons in the hippocampal CA1 region are particularly sensitive to oxidative stress (OS), whereas those in CA3 are resistant. To uncover mechanisms for selective CA1 vulnerability to OS, we...Full Text Available

2007-08-01

299

Genome-Wide Analysis Reveals a Major Role in Cell Fate Maintenance and an Unexpected Role in Endoreduplication for the Drosophila FoxA Gene Fork Head

Zinc is an essential trace metal ion for growth, but an excess of Zn is toxic and microorganisms express diverse resistance mechanisms. To understand global bacterial responses to excess Zn, we conducted...Full Text Available

2005-02-01

300

Genome Sequence of Campylobacter jejuni strain 327, a strain isolated from a turkey slaughterhouse

Transcription factors drive organogenesis, from the initiation of cell fate decisions to the maintenance and implementation of these decisions. The Drosophila embryonic salivary gland...Full Text Available

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301

Genetic screening: The vista of genomic medicine

Campylobacter is one of the leading causes of food-borne gastroenteritis and has a high prevalence in poultry. Campylobacter jejuni subsp. jejuni 327 is a subspecies...Full Text Available

302

Genetic aspects of birth defects: new understandings of old problems

The accelerating development of biochemical and DNA-based diagnostic tests for human genetic conditions in the last decade has engendered a revolution in genetic diagnosis. Both genetic testing and...Full Text Available

2011-01-01

303

Generation of microsatellite repeat families by RTE retrotransposons in lepidopteran genomes

Over the past two decades, combined advances in genetics, developmental biology and biochemistry have transformed the study of human birth defects. This review describes the importance of genome architecture,...Full Text Available

2007-07-01

304

Generation of a BAC-based physical map of the melon genome

BackgroundDeveloping lepidopteran microsatellite DNA markers can be problematical, as markers often exhibit multiple banding patterns and high frequencies of non-amplifying "null"...Full Text Available

305

Gene-gene and gene-environment interactions: new insights into the prevention, detection and management of coronary artery disease

BackgroundCucumis melo (melon) belongs to the Cucurbitaceae family, whose economic importance among horticulture crops is second only to Solanaceae. Melon has high...Full Text Available

306

Gene expression patterns in four brain areas associate with quantitative measure of estrous behavior in dairy cows

Despite the recent success of genome-wide association studies (GWASs) in identifying loci consistently associated with coronary artery disease (CAD), a large proportion of the genetic components of...Full Text Available

307

Functional interaction between the Werner Syndrome protein and DNA polymerase ?

BackgroundThe decline noticed in several fertility traits of dairy cattle over the past few decades is of major concern. Understanding of the genomic factors underlying fertility,...Full Text Available

308

Exploring nervous system transcriptomes during embryogenesis and metamorphosis in Xenopus tropicalis using EST analysis

Werner Syndrome (WS) is an inherited disease characterized by premature onset of aging, increased cancer incidence, and genomic instability. The WS gene encodes a 1,432-amino acid polypeptide (WRN)...Full Text Available

2000-04-25

309

Evidence That Two Major Replicons Comprise the Genome of Staphylococcus Aureus.

BackgroundThe western African clawed frog Xenopus tropicalis is an anuran amphibian species now used as model in vertebrate comparative genomics. It provides the...Full Text Available

310

Distinct signatures of diversifying selection revealed by genome analysis of respiratory tract and invasive bacterial populations

In Staphylococcus aureus, a pronounced shift in position of the acriflavin resistance locus was observed when gene order was determined by marker frequency analysis of cells of various ages. In young cells (2-hour culture), acriflavin resistance was mappe...

1967-01-01

311

Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activity

Many pathogens colonize different anatomical sites, but the selective pressures contributing to survival in the diverse niches are poorly understood. Group A Streptococcus (GAS) is...Full Text Available

2011-03-22

312

Dietary factors related to body weight in adult Vietnamese in the rural area of Haiphong, Vietnam: the Korean Genome and Epidemiology Study (KoGES)

Genome endoreduplication during mammalian development is a rare event for which the mechanism is unknown. It first appears when fibroblast growth factor 4 (FGF4) deprivation induces differentiation...Full Text Available

2008-11-01

313

Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood

The objectives of this study were to examine the association between dietary factors and underweight and overweight adult Vietnamese living in the rural areas of Vietnam. A cross-sectional study of...Full Text Available

2010-06-01

314

DNA Damage during G2 Phase Does Not Affect Cell Cycle Progression of the Green Alga Scenedesmus quadricauda

Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization,...Full Text Available

2011-01-01

315

Comprehensive molecular cytogenetic analysis of sorghum genome architecture: distribution of euchromatin, ...

DNA damage is a threat to genomic integrity in all living organisms. Plants and green algae are particularly susceptible to DNA damage especially that caused by UV light, due to their light dependency...Full Text Available

316

Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae

Cyteogenetic maps of sorghum chromosomes 3-7, 9, and 10 were constructed on the basis of the fluorescence in situ hybridization (FISH) of ~ ... regions of heterchromatin were delimited for all 10 sorghum chrom...

317

Complete characterization of the edited transcriptome of the mitochondrion of Physarum polycephalum using deep sequencing of RNA

BackgroundOncidium spp. produce commercially important orchid cut flowers. However, they are amenable to intergeneric and inter-specific crossing making phylogenetic...Full Text Available

318

Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea, and their metagenomes

RNAs transcribed from the mitochondrial genome of Physarum polycephalum are heavily edited. The most prevalent editing event is the insertion of single Cs, with Us and dinucleotides...Full Text Available

2011-08-01

319

Comparative Transcriptional and Genomic Analysis of Plasmodium falciparum Field Isolates

BackgroundStructured noncoding RNAs perform many functions that are essential for protein synthesis, RNA processing, and gene regulation. Structured RNAs can be detected by comparative...Full Text Available

2010-01-01

320

Common genetic variation and susceptibility to partial epilepsies: a genome-wide association study

Mechanisms for differential regulation of gene expression may underlie much of the phenotypic variation and adaptability of malaria parasites. Here we describe transcriptional variation among culture-adapted...Full Text Available

2009-10-01

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321

Common Familial Colorectal Cancer Linked to Chromosome 7q31: a genome-wide analysis

Partial epilepsies have a substantial heritability. However, the actual genetic causes are largely unknown. In contrast to many other common diseases for which genetic association-studies have successfully...Full Text Available

2010-07-01

322

Chiropteran types I and II interferon genes inferred from genome sequencing traces by a statistical gene-family assembler

Present investigations suggest that approximately 30% of colorectal cancer (CRC) cases arise on the basis of inherited factors. We hypothesize that the majority of inherited factors are moderately...Full Text Available

2008-11-01

323

Capture of genomic DNA on glass microscope slides

BackgroundThe rate of emergence of human pathogens is steadily increasing; most of these novel agents originate in wildlife. Bats, remarkably, are the natural reservoirs of many...Full Text Available

324

Breaking the 1000-gene barrier for Mimivirus using ultra-deep genome and transcriptome sequencing

It is well known that DNA strands bind to silica surfaces in the presence of high concentrations of chaotropic salts. We developed simple methods to evaluate binding and recovery of DNA on flat...Full Text Available

2007-06-15

325

Blood leukocyte DNA hypomethylation and gastric cancer risk in a high-risk Polish population

BackgroundMimivirus, a giant dsDNA virus infecting Acanthamoeba, is the prototype of the mimiviridae family, the latest addition to the family of the nucleocytoplasmic...Full Text Available

326

Analysis of the genome-wide variations among multiple strains of the plant pathogenic bacterium Xylella fastidiosa

Global hypomethylation has been shown to increase genome instability potentially leading to increased cancer risk. We determined whether global methylation in blood leukocyte DNA was associated...Full Text Available

2010-10-15

327

Analysis of genomic differences among Clostridium botulinum type A1 strains

BackgroundThe Gram-negative, xylem-limited phytopathogenic bacterium Xylella fastidiosa is responsible for causing economically important diseases in grapevine,...Full Text Available

328

Analysis of European mtDNAs for Recombination

BackgroundType A1 Clostridium botulinum strains are a group of Gram-positive, spore-forming anaerobic bacteria that produce a genetically, biochemically, and biophysically...Full Text Available

329

An integrative multi-dimensional genetic and epigenetic strategy to identify aberrant genes and pathways in cancer

The standard paradigm postulates that the human mitochondrial genome (mtDNA) is strictly maternally inherited and that, consequently, mtDNA lineages are clonal. As a result of mtDNA clonality, phylogenetic...Full Text Available

2001-01-01

330

An initial comparative map of copy number variations in the goat (Capra hircus) genome

BackgroundGenomics has substantially changed our approach to cancer research. Gene expression profiling, for example, has been utilized to delineate subtypes of cancer, and facilitated...Full Text Available

331

An excess of rare genetic variation in ABCE1 among Yorubans and African-American individuals with HIV-1

BackgroundThe goat (Capra hircus) represents one of the most important farm animal species. It is reared in all continents with an estimated world population of...Full Text Available

332

An Integrated Genomics Approach to Define Niche Establishment by Rhodococcus fascians1[C][W][OA

Signatures of natural selection occur throughout the human genome and can be detected at the sequence level. We have re-sequenced ABCE1, a host candidate gene essential for...Full Text Available

2009-12-01

333

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Rhodococcus fascians is a Gram-positive phytopathogen that induces shooty hyperplasia on its hosts through the secretion of cytokinins. Global transcriptomics using microarrays combined...Full Text Available

2009-03-01

334

Actin-like sequences are present on human X and Y chromosomes.

BackgroundThe production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically...Full Text Available

335

A supervised approach for predicting patient survival with gene expression data

The human genome contains greater than 20 actin-related sequences, six of which at least are expressed as protein. We have shown by blot hybridization the presence of actin-like sequences on both the...Full Text Available

1984-08-01

336

A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells

Rapid development in genomics in recent years has allowed the simultaneous measurement of the expression levels of thousands of genes using DNA microarrays. This has offered tremendous potential...Full Text Available

2010-01-01

337

A novel role of the aryl hydrocarbon receptor (AhR) in centrosome amplification - implications for chemoprevention

Cultured mouse or human embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Chemical or insertional...Full Text Available

2009-04-01

338

A novel custom high density-comparative genomic hybridization array detects common rearrangements as well as deep intronic mutations in dystrophinopathies

BackgroundCentrosome aberrations can cause genomic instability and correlate with malignant progression in common human malignancies such as breast and prostate cancer. Deregulation...Full Text Available

339

A new evolutionary scenario for the Mycobacterium tuberculosis complex

BackgroundThe commonest pathogenic DMD changes are intragenic deletions/duplications which make up to 78% of all cases and point mutations (roughly 20%) detectable...Full Text Available

340

A microarray analysis of the rice transcriptome and its comparison to Arabidopsis

The distribution of 20 variable regions resulting from insertion-deletion events in the genomes of the tubercle bacilli has been evaluated in a total of 100 strains of Mycobacterium tuberculosis,...Full Text Available

2002-03-19

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341

A genomic and proteomic investigation of the impact of preimplantation factor on human decidual cells

Arabidopsis and rice are the only two model plants whose finished phase genome sequence has been completed. Here we report the construction of an oligomer microarray based on the presently...Full Text Available

2005-09-01

342

A genome-wide study of PDZ-domain interactions in C. elegans reveals a high frequency of non-canonical binding

OBJECTIVEPreimplantation factor (PIF) is a novel, 15 amino acid peptide, secreted by viable embryos. This study aims to elucidate PIF’s effects in human endometrial...Full Text Available

2010-05-01

343

A Response Regulator That Represses Transcription of Several Virulence Operons in the Group A Streptococcus

BackgroundProteins may evolve through the recruitment and modification of discrete domains, and in many cases, protein action can be dissected at the domain level. PDZ domains are...Full Text Available

344

A Proposal for a Coordinated Effort for the Determination of Brainwide Neuroanatomical Connectivity in Model Organisms at a Mesoscopic Scale

A search for homologs of the Bacillus subtilis PhoP response regulator in the group A streptococcus (GAS) genome revealed three good candidates. Inactivation of one of these, recently...Full Text Available

1999-06-01

345

A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence.

In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function....Full Text Available

2009-03-01

346

High-Resolution Computed Tomography with Single-Slice Computed Tomography and 16-Channel Multidetector Computed Tomography: A Comparison Regarding Visibility and Motion Artifacts

A DNA fragment located on the 3' side of the Coxiella burnetii htpAB operon was determined by Southern blotting to exist in approximately 19 copies in the Nine Mile I genome. The DNA sequences of this...Full Text Available

1992-09-01

347

Search for a Methanopyrus-proximal last universal common ancestor based on comparative-genomic analysis

Background: High-resolution computed tomography is the image procedure of choice in the evaluation of interstitial lung disease. Multidetector-row computed tomography provides the possibility of simultaneous reconstruction of thin and thick slices from the same raw data, acquired from one single series. Thus, it may be tempting to exclude the step-and-shoot series. Purpose: To compare high-resolution computed tomography (HRCT step-and-shoot) from single-slice CT (SSCT) and 16-channel multidetector CT (MDCT) in terms of visibility and motion artifacts, and to investigate whether thin images reconstructed from helical MDCT are equal to or better than conventional HRCT by SSCT in terms of visibility and motion artifacts. Material and Methods: 20 patients underwent HRCT step-and-shoot by SSCT (SSCT step-and-shoot) and MDCT (MDCT step-and-shoot), and a helical MDCT acquisition (MDCT ...

2007-11-01

348

British Library Electronic Table of Contents (United Kingdom)

The origin of life is a long-standing mystery puzzling many people. This mystery possesses not only philosophical but also important biological significance. To unveil this mystery, the searches for the root of life, or the last universal common ancestor (LUCA), based on comparative-genomic analysis have been intensively performed on rRNAs, tRNAs and proteins sequences. The current search pointed to a Methanopyrus-proximal LUCA, which opens up the reconstruction of Lucan biology and helps to delineate the evolutionary pathways.

2011-01-01

349

The two power limits conditioning step frequency in human running.

A simplified two step thermal analysis method for Wolsung (CANDU) spent fuel dry storage canister

1. At high running speeds, the step frequency becomes lower than the apparent natural frequency of the body's bouncing system. This is due to a relative increase of the vertical component of the muscular...Full Text Available

1991-06-01

350

A simplified two step thermal analysis method for Wolsung (CANDU) spent fuel dry storage canister

A simplified two step thermal analysis method has been develop to evaluate (1) the mean temperature of the CANDU fuel bundles within a fuel basket in a given spent fuel dry storage canister by HEATING5 code with additional input data and heat transfer correlations in the step-1 analysis and (2) the maximum fuel rod temperature within a CANDU 37-element fuel bundle by MAXROT code developed here for step-2 analysis. In addition, the results of sample analysis is performed to examine the parametric effects of the site-specific ambient conditions on the maximum fuel temperature within a canister are presented. The comparison between the results of step-1 analysis and the mock-up test, in particular, is quite satisfactory. In essence, the two-step thermal analysis method proposed here is a code package that used the HEATING5 and MAXROT codes, respectively, for step-1 ...

1993-03-01

351

Ecotoxicology of Explosives

A simplified two step thermal analysis method has been develop to evaluate (1) the mean temperature of the CANDU fuel bundles within a fuel basket in a given spent fuel dry storage canister by HEATING5 code with additional input data and heat transfer correlations in the step-1 analysis and (2) the maximum fuel rod temperature within a CANDU 37-element fuel bundle by MAXROT code developed here for step-2 analysis. In addition, the results of sample analysis is performed to examine the parametric effects of the site-specific ambient conditions on the maximum fuel temperature within a canister are presented. The comparison between the results of step-1 analysis and the mock-up test, in particular, is quite satisfactory. In essence, the two-step thermal analysis method proposed here is a code package that used the HEATING5 and MAXROT codes, respectively, for step-1 ...

1993-01-01

352

Two-step or finite-range effects in charge-exchange reactions

Managing sites contaminated with munitions constituents is an international challenge. Although the choice of approach and the use of Ecological Risk Assessment (ERA) tools may vary from country to country, the assurance of quality and the direction of ecotoxicological research are universally recognized as shared concerns. Drawing on a multidisciplinary team of contributors, 'Ecotoxicology of Explosives' provides comprehensive and critical reviews available to date on fate, transport, and effects of explosives. The book delineates the state of the science of the ecotoxicology of explosives, past, present, and recently developed. It reviews the accessible fate and ecotoxicological data for energetic materials (EMs) and the methods for their development. The chapters characterize the fate of explosives in the environment, then provide information on their ecological effects in key environmental media, including aquatic, ...

2009-04-01

353

The Value of Information The Metaphysics of Information The - NASA

... charge-exchange reactions dwba finite-range interactions helium 3 reactions

1975-04-07

354

The South Asia Water Analysis Network : introduction to the cooperative monitoring center, overview of the SAWAN project, expectations, and next steps.

Wikileaks was inevitable. The Dynamics of. Information. Take advantage of the eddy currents. You can't step in the same river ...

355

PRODUCTION ENGINEERING MEASURE RELIABILITY ...

No abstract prepared.

2004-06-01

356

... without the problem of purple plague. The Au-Ni-In evaporated ... surface and bonding degradation (purple plague) St step-stress ...

1963-09-30

357

NRL Fact Book 2010

... Tactical Electronic Warfare Division (Code 5700) Visualization Laboratory Transportable step frequency radar Vehicle development laboratory ...

2011-05-15

358

NRL Fact Book

Effect of Pass Schedule on the Microstructures and Mechanical Properties of Multi-step Cold Rolled High Carbon Steel Wires

... Tactical Electronic Warfare Division (Code 5700) Visualization Laboratory Transportable step frequency radar Vehicle development laboratory ...

2011-05-15

359

Contamination preventive method and decontaminating method for equipment

Full Text Available

2011-06-01

360

Circuit breaker lock out assembly

The method of the present invention comprises a step of previously coating a positive type resist on the surface of equipment, a step of solidifying the coated positive type resist and a step of bringing the coated equipment to circumstances for use and cleaning the positive type resist. With such procedures, the amount of radioactive materials deposited on the equipment can be reduced, and redeposition of nuclides on the surface to be cleaned can be prevented. (T.M.)

1998-12-04

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Vegetation Growth Monitoring Under Coal Exploitation Stress by Remote Sensing in the Bulianta Coal Mining Area

A lock out assembly for a circuit breaker which consists of a generally step-shaped unitary base with an aperture in the small portion of the step-shaped base and a roughly S shaped retaining pin which loops through the large portion of the step-shaped base. The lock out assembly is adapted to fit over a circuit breaker with the handle switch projecting through the aperture, and the retaining pin projecting into an opening of the handle switch, preventing removal.

1983-05-18

362

British Library Electronic Table of Contents (United Kingdom)

Coal exploitation inevitably damages the natural ecological environment through large scale underground exploitation which exhausts the surrounding areas and is the cause of surface subsidence and cracks. These types of damage seriously lower the underground water table. Deterioration of the environment has certainly an impact on and limits growth of vegetation, which is a very important indicator of a healthy ecological system. Dynamically monitoring vegetation growth under coal exploitation stress by remote sensing technology provides advantages such as large scale coverage, high accuracy and abundant information. A scatter plot was built by a TM (Thematic Mapper) infrared and red bands. A detailed analysis of the distributional characteristics of vegetation pixels has been carried out. ...

2007-01-01

363

The coexistence of fish species in streams: relationships between assemblage attributes and trophic and environmental variables

British Library Electronic Table of Contents (United Kingdom)

Diet overlap and niche breadth are well-known species traits from trophic ecology that can assist in explaining how species interact and coexist as well as the ecological mechanisms that influence biodiversity. In the present study, we analyzed the relationships between these trophic variables and indicators of resource availability with some attributes of fish assemblages (species richness, Shannon diversity index, evenness, density and individual body size). The physical and chemical characteristics of the biotopes (topography, water quality and conservation of slopes) were examined to identify possible patterns. Monthly sampling using electrofishing was conducted in 2003 along five streams located in the Cuiab? River watershed. The relationships between environmental variables and attri...

2011-01-01

364

System, economy and ecology viewpoints of the Krsko NPP lifetime extension

Environmental impact of a nuclear plant on Mississippi River biota in an ecological recovery zone near Red Wing, Minnesota. Final report

Krsko NPP plant life extension was analysed and evaluated with respect to system, economy and ecology viewpoints. From the system perspective it was established that also in the extended lifetime the plant will remain in operation as a base load electricity supplier. The systematic review was performed to determine its overall competitiveness against advanced coal, gas and new nuclear units. The analysis considered also hydro and renewable sources. Analysis and evaluations resulted in the conclusion that the Krsko NPP lifetime extension is the most effective alternative for base load production due to small additional capital investments, low fuel costs, no new siting requirements, lowest climate and environmental impact, and reliable and safe operation. (author)

2007-09-10

365

Environmental Science and Research Foundation annual technical report to DOE-ID, January , 1995--December 31, 1995

The biota of the Mississippi River in an ecological recovery zone near Red Wing, MN was studied before and after start-up of a nuclear plant to acquire baseline data and to estimate changes due to thermal addition. Thermal addition produced significant decreases in primary production in a plant region during summer month periods when water temperatures were near 36C. Extensive fisheries studies and sonar tagging/tracking of S. vitreum were performed. Comparison of fisheries results with other riverine, lake, and pond studies indicated a favorable environment for the success of the fish species studied. Qualitative macroinvertebrate surveys were conducted.

366

Differential facilitative and competitive effects of a dominant macrophyte in grazed subtropical wetlands

The foundation conducts an environmental monitoring and surveillance program over an area covering much of the upper Snake River Plain and provide environmental education and support services related to INEL natural resource issues. Also, the foundation, with its university affiliates, conducts ecological and radioecological research on the Idaho National Environmental Research Park. This research benefits major DOE-ID programs including waste management, environmental restoration, spent nuclear fuels, and land management issues. Major accomplishments during CY1995 can be divided into five categories: environmental surveillance program, environmental education, environmental services and support, ecological risk assessment, and research benefitting the DOE-ID mission.

367

British Library Electronic Table of Contents (United Kingdom)

Summary 1.-Plant-plant interactions fluctuate between competition and facilitation depending upon ecological conditions and species traits. Facilitative interactions are expected to increase in frequency via associational defences with increasing consumer pressure. The ability of species to cope with competition and/or ecological stressors may alter the outcome of plant-plant interactions. 2.-We conducted a transplant experiment to determine if native and non-native grasses and forbs respond similarly to interactions with Juncus effusus L., an unpalatable benefactor species, along a grazing intensity gradient in two contrasting pasture types: intensively managed and semi-natural. We expected competitive taller, erect species (grasses) and non-natives to obtain stronger facilitative effects...

2011-01-01

368

Assessing farm-level agricultural sustainability over a 60-year period in rural eastern India

British Library Electronic Table of Contents (United Kingdom)

Agricultural sustainability is a vital parameter to be ascertained locally and globally if food security is to be achieved and maintained. Agricultural sustainability is the combined product of social, economic and ecological sustainability. It is also a function of temporal and spatial variations, a fact which indicates that area-specific sustainability indices need to be designed. We present here an Agricultural Sustainability Index (ASI) for rural eastern India and use it to calculate the ASI for 150 farms for three decades over a 60-year period, viz., 1950?1960, 1980?1990 and 2000?2010 for a representative Indian village of Gangapur (25?83?N, 85?65?E). The ASI was calculated using 30 variables, 10 each of social, economic and ecological sustainability. An extensive questionnaire-based ...

2011-01-01

369

Wiley::Automotive Electricity: Electric Drive

Sustainable pest control - comparing tritrophic interactions in organic and conventional production systems

... Another solution, hybrid vehicles, combine two sources of energy (electric and chemical), reducing the global consumption of fossil fuels. Fuel cell vehicles are also one of the most promising technologies for the future, with the capacity to use any fuel - hydrogen being the ideal fuel ecologically, but constrained by infrastructure and storage issues. This book explores all these different solutions for moving our vehicles from fossil ...

370

Environmental Research Database

DescriptionThis cross-disciplinary project will combine chemical, ecological and modelling techniques to determine whether cabbages grown under an organic regime differ in terms of pest dynamics and plant chemistry. There is increasing pressure to de-intensify agricultural practice and organic approaches are becoming more popular and widely adopted. However, there are very few, if any, detailed scientific investigations into the claims made about improved pest control, reduced environmental impact and be [continued...

2009-01-31

371

Study on compressed biogas and its application to the compression-ignition dual-fuel engine. Technical report

Review of the 1996 Pacific Basin Conference and future outlook

This paper introduces in detail the compression performance of biogas, the application of compressed biogas to the compression-ignition engine and the possibility as well as the necessity of using compressed biogas. Moreover, the paper also goes further into some problems about the power increasing, efficiency raising and their social, economic and ecological beneficial results, when the compressed biogas is used in the compression ignition-dual fuel engine.

1987-01-01

372

Particulate air pollution and chronic ischemic heart disease in the eastern United States: a county level ecological study using satellite aerosol data

Highlights of the meeting are briefly summarized in this paper. Most of the papers presented at the meeting dealt with remediation and pollution prevention practices. A major focus of the technical sessions was on the identification of pollution sources. Identification of exposures to specific chemicals with disease outcomes was also discussed. Other papers focused on ecological exposures and their effects on wildlife to identify the presence of contaminants. 4 refs.

1996-12-31

373

BackgroundThere are several known factors that cause ischemic heart disease. However, the part played by air pollution still remains something of a mystery. Recent attention has...Full Text Available

374

NAME=\\

Industrial ecology at Lawrence Livermore National Laboratory summary statement

...Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain Assemblages of plants and invertebrate animals of tumulus ...organic mound) springs of the Swan Coastal Plain [Skip navigation links] About us | Contact us | Publications |...ecological communities Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain Advice to the Minister for the ...of the 'Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain' community sufficient to distinguish it from ...

375

INTRODUCTION OF ATRAZINE-DEGRADING PSEUDOMONAS SP. STRAIN ADP TO ENHANCE PHYTOREMEDIATION OF ATRAZINE

This statement summarizes Lawrence Livermore National Laboratory`s committment to making important scientific, technological, and business contributions to global sustainability. The quest has many aspects, some socio-political or economic and some technological, and some in which the soft and hard sciences become indistinguishable, as in visionary national strategies, like Holland`s, and futuristic regional and city development plans, like those of Kagoshima and Chattanooga.

1996-05-21

376

FY 1999 Laboratory Directed Research and Development annual report

Atrazine (ATR) has been widely applied in the US and Mid Western states. Recently, public health and ecological concerns have been raised about contamination of surface and ground water by ATR and its chlorinated metabolites, due to their toxicity and potential carcinogenic or endocrinology effects....

377

Climate Change and Agricultural Vulnerability

A short synopsis of each project is given covering the following main areas of research and development: Atmospheric sciences; Biotechnology; Chemical and instrumentation analysis; Computer and information science; Design and manufacture engineering; Ecological science; Electronics and sensors; Experimental technology; Health protection and dosimetry; Hydrologic and geologic science; Marine sciences; Materials science; Nuclear science and engineering; Process science and engineering; Sociotechnical systems analysis; Statistics and applied mathematics; and Thermal and energy systems.

2000-06-13

378

Environmental Research Database

After the introduction Chapter 2 presents details of the ecological-economic analysis based on the FAO/IIASA agro-ecological zones (AEZ) approach for evaluation of biophysical limitations and agricultural production potentials, and IIASA's Basic Linked System (BLS) for analyzing the world's food economy and trade system. The BLS is a global general equilibrium model system for analyzing agricultural policies and food system prospects in an international setting. BLS views national agricultural systems as embedded in national economies, which interact with each other through trade at the international level. The combination of AEZ and BLS provides an integrated ecological-economic framework for the assessment of the impact of climate change. We consider climate scenarios based on experiments with four General Circulation Models (GCM), and we assess the four basic socioeconomic development pathways and ...

2002-08-01

379

CCMAP Contract

DescriptionCEH - Quest CCMAP see http://quest.bris.ac.uk/research/themes/CCMAP.html CCMAP (Climate-carbon modelling, assimilation and prediction) Leader: Dr. Eleanor Blyth (CEH, Wallingford) Overview and Goals CCMAP is one of QUEST's Theme 1 projects and is about to be commissioned to the Centre for Ecology and Hydrology at Wallingford. The Principal Investigator will be Dr. Eleanor Blyth, with subcontracts to the Universities of Bristol (Dr. Wolfgang Knorr, Earth Sciences and QUEST; Andy Ridgw [continued...

380

Bibliography of prosopis

A case study in atmospheric lead pollution of Northern-German coastal regions

References covering the years 1904-80 are listed under the following headings: cultivation and occurrence (India and Pakistan, Africa, South America, Pacific, Middle East); Taxonomy, morphology, variation and selection; Reference works and reviews; Ecology of Prosopis (General effects on surrounding soil and vegetation): Physiology (General, Roots, Growth, Hydrology, Saline tolerance); Control of mesquite: Propagation (Germination and other nursery techniques, Vegetative propagation): and Utilization (General, Chemical analyses, Food and Ethnobiology, Fodder, Wood, Charcoal, Gum, Paper). 141 references.

1981-01-01

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381

The enhanced genomic instability was induced by alpha particle and low-energy ion irradiation in somatic cells of Arabidopsis thaliana

Transport and deposition of atmospheric lead over the coastal zone of Northern Germany are investigated. It is shown that marked differences in the impact of the ecologically valuable wadden sea areas can occur between summer and winter time. Due to the formation of sea breeze systems in summer the coastal zone is likely to be less stressed than in winter when the pollutant is confined to a shallow layer above ground. (orig.) 10 refs.

1998-12-31

382

Reproductive Ecology of Yakima River Hatchery and Wild Spring Chinook; Yakima/Klickitat Fisheries Project Monitoring and Evaluation, 2004-2005 Annual Report.

Although low-energy ion radiation has been proven to have a wide range of biological effects and led to fruitful achievements as a new mutagenic source for genetic modification, there still exist some disputes about its mutagenic mechanisms because of its short-penetrating property. In present research, Arabidopsis thaliana transgenic for GUS recombination substrate was used to evaluate the genomic instability induced by irradiations of alpha particle (3.3MeV) and Low-energy-Argon ion (30 KeV). A pronounced effects of alpha particle irradiation to Arabidopsis thaliana seedlings and Argon ion irradiation to seeds on the somatic homologous recombination frequency (sHRF) were reported. The sHRFs increased 1.88-fold and 2.42-fold, respectively, which indicated that the short-penetrating radiation could effectively induce the plant genomic instability in either dry seeds or seedlings with active metabolism. The local alpha particle irradiation of ...

2008-08-12

383

The mouse formin (Fmn) gene: Genomic structure, novel exons, and genetic mapping

This report is intended to satisfy two concurrent needs: (1) provide a contract deliverable from Oncorh Consulting to the Washington Department of Fish and Wildlife (WDFW), with emphasis on identification of salient results of value to ongoing Yakima/Klickitat Fisheries Project (YKFP) planning and (2) summarize results of research that have broader scientific relevance. This is the fourth in a series of reports that address reproductive ecological research and monitoring of spring chinook populations in the Yakima River basin. This annual report summarizes data collected between April 1, 2004 and March 31, 2005 and includes analyses of historical baseline data, as well. Supplementation success in the Yakima Klickitat Fishery Project's (YKFP) spring chinook (Oncorhynchus tshawytscha) program is defined as increasing natural production and harvest opportunities, while keeping adverse ecological interactions and genetic impacts within ...

2005-05-01

384

Genetic effects of introgression genomic components from Sea Island cotton (Gossypium barbadense L.) on fiber related traits in upland cotton (G. hirsutum L.)

Mutations in the mouse formin (Fmn) gene, formerly known as the limb deformity (ld) gene, give rise to recessively inherited limb deformities and renal malformations or aplasia. The Fmn gene encodes many differentially processed transcripts that are expressed in both adult and embryonic tissues. To study the genomic organization of the Fmn locus, we have used Fmn probes to isolate and characterize genomic clones spanning 500 kb. Our analysis of these clones shows that the Fmn gene is composed of at least 24 exons and spans 400 kb. We have identified two novel exons that are expressed in the developing embryonic limb bud as well as adult tissues such as brain and kidney. We have also used a microsatellite polymorphism from within the Fmn gene to map it genetically to a 2.2-cM interval between D2Mit58 and D2Mit103. 36 refs., 6 figs., 1 tab.

1997-02-01

385

British Library Electronic Table of Contents (United Kingdom)

The germplasm with exotic genomic components especially from Sea Island cotton (Gossypium barbadense L. Gb) is the dominant genetic resources to enhance fiber quality of upland cotton (G. hirsutum L., Gh). Due to low efficiency of phenotypic evaluation and selection on fiber quality, genetic dissection of favorable alleles using molecular markers is essential. Genetic dissection on putative Gb introgressions related to fiber traits were conducted by SSR markers with mapping populations derived from a cross between Luyuan343 (LY343), a superior fiber quality introgression line (IL) with genomic components from Gb, and an elite Upland cotton cv. Lumianyan#22 (LMY22). Among 82 polymorphic loci screened out from 4050 SSRs, 42 were identified as putative introgression alleles. A total of 29 fib...

2011-01-01

386

Gene discovery in the Acanthamoeba castellanii genome

Evolutionary dynamics of Newcastle disease virus

Acanthamoeba castellanii is a free-living amoeba found in soil, freshwater, and marine environments and an important predator of bacteria. Acanthamoeba castellanii is also an opportunistic pathogen of clinical interest, responsible for several distinct diseases in humans. In order to provide a genomic platform for the study of this ubiquitous and important protist, we generated a sequence survey of approximately 0.5 x coverage of the genome. The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A. castellanii to inhabit adversity of environments. Alginate lyase may provide access to bacteria within biofilms by breaking down the biofilm matrix, and polyhydroxybutyrate depolymerase may facilitate utilization of the bacterial storage compound polyhydroxybutyrate as a food source. Enzymes for the ...

2005-08-01

387

Complete genome sequence of Conexibacter woesei type strain (ID131577T)

A comprehensive dataset of NDV genome sequences was evaluated using bioinformatics to characterize the evolutionary forces affecting NDV genomes. Despite evidence of recombination in most genes, only one event in the fusion gene of genotype V viruses produced evolutionarily viable progenies. The codon-associated rate of change for the six NDV proteins revealed that the highest rate of change occurred at the fusion protein. All proteins were under strong purifying (negative) selection; the fusion protein displayed the highest number of amino acids under positive selection. Regardless of the phylogenetic grouping or the level of virulence, the cleavage site motif was highly conserved implying that mutations at this site that result in changes of virulence may not be favored. The coding sequence of the fusion gene and the genomes of viruses from wild birds displayed higher yearly rates of change in virulent viruses than in ...

2009-08-15

388

The genetic basis of salinity tolerance in Arctic charr (Salvelinus alpinus).

The genus Conexibacter (Monciardini et al. 2003) represents the type genus of the family Conexibacteraceae (Stackebrandt 2005, emend. Zhi et al. 2009) with Conexibacter woesei as the type species of the genus. C. woesei is a representative of a deep evolutionary line of des-cent within the class Actinobacteria. Strain ID131577T was originally isolated from temperate forest soil in Gerenzano (Italy). Cells are small, short rods that are motile by peritrichous fla-gella. They may form aggregates after a longer period of growth and, then as a typical charac-teristic, an undulate structure is formed by self-aggregation of flagella with entangled bacteri-al cells. Here we describe the features of the organism, together with the complete sequence and annotation. The 6,359,369 bp long genome of C. woesei contains 5,950 protein-coding and 48 RNA genes and is part of the Genomic Encyclopedia of Bacteria and Archaea project.

2010-01-01

389

ABSTRACT: BACKGROUND: The capacity to maintain internal ion homeostasis amidst changing conditions is particularly important for teleost fishes whose reproductive cycle is dependent upon movement from freshwater to seawater. Although the physiology of seawater osmoregulation in mitochondria-rich cells of fish gill epithelium is well understood, less is known about the underlying causes of inter- and intraspecific variation in salinity tolerance. We used a genome-scan approach in Arctic charr (Salvelinus alpinus) to map quantitative trait loci (QTL) correlated with variation in four salinity tolerance performance traits and six body size traits. Comparative genomics approaches allowed us to infer whether allelic variation at candidate gene loci (e.g., ATP1alpha1b, NKCC1, CFTR, and cldn10e) could have underlain observed variation. RESULTS: Combined parental analyses yielded genome-wide significant QTL on linkage groups 8, 14 ...

2011-09-21

390

Universal Similarity

Summary of the OA biomarkers workshop 2010 - genetics and genomics: new targets in OA

We survey a new area of parameter-free similarity distance measures useful in data-mining, pattern recognition, learning and automatic semantics extraction. Given a family of distances on a set of objects, a distance is universal up to a certain precision for that family if it minorizes every distance in the family between every two objects in the set, up to the stated precision (we do not require the universal distance to be an element of the family). We consider similarity distances for two types of objects: literal objects that as such contain all of their meaning, like genomes or books, and names for objects. meaning, like genomes or books, and names for objects. The latter may have literal embodyments like the first type, but may also be abstract like ``red'' or ``christianity.'' For the first type we consider a family of computable distance measures corresponding to parameters expressing similarity according to particular features between ...

2005-01-01

391

British Library Electronic Table of Contents (United Kingdom)

On November fourth and fifth 2010 a group of more than 100 international investigators gathered in Atlanta for the second Osteoarthritis (OA) Biomarkers Global Initiative workshop titled ''Genetics and Genomics: New Targets in OA''. The first workshop took place in April 2009 and focused on in vitro (soluble) biomarkers whilst the third and final workshop will take place in 2012 and will focus on imaging biomarkers. The OA Research Society International (OARSI) has organized the workshops. In addition to OARSI, the National Institute of Arthritis, Musculoskeletal and Skin Diseases, the Arthritis Foundation, Amgen, Genzyme, the American Orthopaedic Society for Sports Medicine and Pfizer sponsored the second meeting. It was clear from this meeting that experiments in the genetics, epigenetic...

2011-01-01

392

Selective changes of retroelement expression in human prostate cancer

British Library Electronic Table of Contents (United Kingdom)

Retroelements constitute a large part of the human genome. These sequences are mostly silenced in normal cells, but genome-wide DNA hypomethylation in cancers might lead to their re-expression. Whether this re-expression really occurs in human cancers is largely unkown. We therefore investigated expression and DNA methylation of several classes of retroelements in human prostate cancer tissues and cell lines by quantitative reverse transcription-polymerase chain reaction and pyrosequencing, respectively. The most striking finding was strong and generalized increased expression of the HERV-K_22q11.23 provirus in cancers, including de novo expression of a spliced accessory Np9 transcript in some tumors. In parallel, DNA methylation in the long terminal repeat (LTR) decreased. Conversely, HER...

2011-01-01

393

Introduction to the Special Issue: Human Linkage Studies for Behavioral Traits

British Library Electronic Table of Contents (United Kingdom)

In the post Genome era, the aim of behavior genetics has shifted from estimating the relative contributions of genes and environmental factors to (co-)variation in human complex traits, to localization of genes and identification of functional genetic variants. This special issue reflects this transition and presents fifteen papers that report on genome-wide linkage scans for complex traits in humans and on methodological tools and innovations. Six papers focus on cognition and report overlapping linkage peaks on chromosomes 6p and 14p. Papers on addictive behavior, i.e. smoking and alcohol dependence and its endophenotypes, find moderate LOD scores on chromosomes 6p, 5q, 4p and 7q, respectively. Three papers concentrate on emotionality, depression and loneliness and examine chromosomes 2q...

2006-01-01

394

Genomic architecture of aggression: Rare copy number variants in intermittent explosive disorder

British Library Electronic Table of Contents (United Kingdom)

Abstract Copy number variants (CNVs) are known to be associated with complex neuropsychiatric disorders (e.g., schizophrenia and autism) but have not been explored in the isolated features of aggressive behaviors such as intermittent explosive disorder (IED). IED is characterized by recurrent episodes of aggression in which individuals act impulsively and grossly out of proportion from the involved stressors. Previous studies have identified genetic variants in the serotonergic pathway that play a role in susceptibility to this behavior, but additional contributors have not been identified. Therefore, to further delineate possible genetic influences, we investigated CNVs in individuals diagnosed with IED and/or personality disorder (PD). We carried out array comparative genomic hybridizati...

2011-01-01

395

Genome-wide association study identifies variants in the CFH region associated with host susceptibility to meningococcal disease

British Library Electronic Table of Contents (United Kingdom)

Meningococcal disease is an infection caused by Neisseria meningitidis. Genetic factors contribute to host susceptibility and progression to disease, but the genes responsible for disease development are largely unknown. We report here a genome-wide association study for host susceptibility to meningococcal disease using 475 individuals with meningococcal disease (cases) and 4,703 population controls from the UK. We performed, in Western European and South European cohorts (consisting of 968 cases and 1,376 controls), two replication studies for the most significant SNPs. A cluster of complement factor SNPs replicated independently in both cohorts, including SNPs within complement factor H (CFH) (rs1065489 (p.936D

2010-01-01

396

Complete genome sequences of Brucella melitensis strains M28 and M5-90, with different virulence backgrounds.

An integrated genome research network for studying the genetics of alcohol addiction

Brucella melitensis is a Gram-negative coccobacillus bacteria belonging to the Alphaproteobacteria subclass. It is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. The B. melitensis strain M5-90, a live attenuated vaccine cultured from the B. melitensis virulent strain M28, has been an effective tool to control brucellosis in goats and sheep in China. Here we report the complete genome sequences of B. melitensis M28 and M5-90, strains with different virulence backgrounds, which will serve as a valuable reference for future studies. PMID:21478357

2011-04-08

397

British Library Electronic Table of Contents (United Kingdom)

Abstract Alcohol drinking is highly prevalent in many cultures and contributes to the global burden of disease. In fact, it was shown that alcohol constitutes 3.2% of all worldwide deaths in the year 2006 and is linked to more than 60 diseases, including cancers, cardiovascular diseases, liver cirrhosis, neuropsychiatric disorders, injuries and foetal alcohol syndrome. Alcoholism, which has been proven to have a high genetic load, is one potentially fatal consequence of chronic heavy alcohol consumption, and may be regarded as one of the most prevalent neuropsychiatric diseases afflicting our society today. The aim of the integrated genome research network -Genetics of Alcohol Addiction--which is a German inter-/trans-disciplinary life science consortium consisting of molecular biologists,...

2010-01-01

398

An optical fiber methane sensing system employing a two-step reference measuring method

British Library Electronic Table of Contents (United Kingdom)

An optical fiber methane sensing system utilizing a super-luminescent diode (SLD) in the 1.33-mm band and a fiber Fabry-Perot tunable filter (FFP-TF) is developed. A two-step reference measuring method, which is based on differential absorption technique, is employed; a reference channel is introduced to eliminate the fluctuation of the illuminant spectrum, and the reference wavelength acquired by tuning the FFP-TF is introduced to eliminate the environmental influence. The two-step reference measuring method has improved the reliability and stability of the system. A minimum detectable methane concentration of 0.3% has been experimentally achieved.

2008-01-01

399

Advantages and limitations of the SETS method. [PWR; BWR

US Army Self-Development: Enhancer or Barrier to Leader ...

The stability-enchancing two-step (SETS) method has been used successfully in the Transient Reactor Analysis Code (TRAC) for several years. The method consists of a basic semi-implicit step combined with a stabilizer step that, taken together, eliminate the material Courant stability limit associated with standard semi-implicit numerical methods. This approach toward stability requires significantly fewer computational operations than a fully implicit method, but currently maintains the first-order accuracy in space and time of its semi-implicit predecessors.

1983-01-01

400

Two-step biodiesel production from Calophyllum inophyllum oil: Optimization of modified b-zeolite catalyzed pre-treatment

... This must be taken a step further in counseling and other feedback and ... Record System Power Point Presentation ,www.armyppt.com/ncoer/6.htm ...

2003-05-01

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401

British Library Electronic Table of Contents (United Kingdom)

In this study, a two-step process was developed to produce biodiesel from Calophyllum inophyllum oil. Pre-treatment with phosphoric acid modified b-zeolite in acid catalyzed esterification process preceded by transesterification which was done using conventional alkali catalyst potassium hydroxide (KOH). The objective of this study is to investigate the relationship between the reaction temperatures, reaction time and methanol to oil molar ratio in the pre-treatment step. Central Composite Design (CCD) and Response Surface Methodology (RSM) were utilized to determine the best operating condition for the pre-treatment step. Biodiesel produced by this process was tested for its fuel properties.

2011-01-01

402

The stability of biological standards

The 60 Minute Network Security Guide (First Steps Towards a ...

The authors emphasize the importance of stability in biological standards and discuss the steps taken to ensure the stability of International Standards and the theoretical basis of the various stability...Full Text Available

1956-01-01

403

Repriming the actomyosin crossbridge cycle

... UNCLASSIFIED The 60 Minute Network Security Guide ... 15. SUBJECT TERMS IATAC Collection; information security; network security 16. ...

2001-10-16

404

N-Body Code with Adaptive Mesh Refinement

The central features of the mechanical cycle that drives the contraction of muscle are two translational steps: the working stroke, whereby an attached myosin crossbridge moves relative to the actin...Full Text Available

2004-08-31

405

How frugal is mother nature with haplotypes?

We have developed a simulation code with the techniques which enhance both spatial and time resolution of the PM method for which the spatial resolution is restricted by the spacing of structured mesh. The adaptive mesh refinement (AMR) technique subdivides the cells which satisfy the refinement criterion recursively. The hierarchical meshes are maintained by the special data structure and are modified in accordance with the change of particle distribution. In general, as the resolution of the simulation increases, its time step must be shortened and more computational time is required to complete the simulation. Since the AMR enhances the spatial resolution locally, we reduce the time step locally also, instead of shortening it globally. For this purpose we used a technique of hierarchical time steps (HTS) which changes the time step, from particle to particle, depending on the size of the cell in ...

2001-01-01

406

Habitat Selection by Mountain Plovers in Shortgrass Steppe

Motivation: Inference of haplotypes from genotype data is crucial and challenging for many vitally important studies. The first, and most critical step, is the ascertainment of a biologically...Full Text Available

2009-01-01

407

Flow field and heat transfer associated with laminar flow over a forward-facing step

... the area covered by the point-count grid, stopping approximately every 100 m, stepping away from the vehicle, and scanning with binoculars for mountain plovers. Once ... ...

408

Far-Infrared Spontaneous Intraband Emission from Laser ...

The flow and heat transfers associated with a plane laminar flow past a forward-facing step were analyzed using a power-law numerical scheme combined with a false vorticity-stream function approach. To improve the traditional wall-vorticity boundary condition, a novel method, based on an accurate description of the nonslip wall condition, was developed and utilized. The convergence for a 56 x 49 grid system was obtained in about 350 iterations. The computed reattachment distances in the upper separated region agree with the available experimental data for a blunt plate. The heat transfer augmentation is significant across the step; however, it is counterbalanced by the deterioration of heat transfer immediately upstream of the step.

1986-01-01

409

Bankart Repair to Correct Shoulder Instability

... The fact, that such a spontaneous radiation have been observed, may be considered as the first step of development of the FIR laser based on ...

1998-06-01

410

Medline Plus

... very difficult to help get better and to rehabilitate and sometimes even need surgery. 00:04:21 ... step of treatment, from our standpoint, is to rehabilitate, to strengthen the shoulder. And the real key ...

411

The normal developmental regulation of a cloned sgs3 'glue' gene chromosomally integrated in Drosophila melanogaster by P element transformation.

The Complete Genome Sequence of Yersinia pseudotuberculosis IP31758, the Causative Agent of Far East Scarlet-Like Fever

A 7-kb genomic segment containing the coding sequence for the Drosophila melanogaster Formosa variant of salivary gland secretion protein 3 (sgs3) has been inserted into the snw y, bw, st strain of...Full Text Available

1983-01-01

412

The Acidic Domain of Hepatitis C Virus NS4A Contributes to RNA Replication and Virus Particle Assembly?

The first reported Far East scarlet-like fever (FESLF) epidemic swept the Pacific coastal region of Russia in the late 1950s. Symptoms of the severe infection included erythematous skin rash and desquamation,...Full Text Available

2007-08-01

413

Structural Chemistry of Human SET Domain Protein Methyltransferases

Hepatitis C virus NS3-4A is a membrane-bound enzyme complex that exhibits serine protease, RNA helicase, and RNA-stimulated ATPase activities. This enzyme complex is essential for viral genome replication...Full Text Available

2011-02-01

414

Sequencing and Comparative Genomic Analysis of pK29, a 269-Kilobase Conjugative Plasmid Encoding CMY-8 and CTX-M-3 ?-Lactamases in Klebsiella pneumoniae?

There are about fifty SET domain protein methyltransferases (PMTs) in the human genome, that transfer a methyl group from S-adenosyl-L-methionine (SAM) to substrate lysines on histone tails or other peptides. A number of structures in complex with cofactor, substrate, or inhibitors revealed the mechanisms of substrate recognition, methylation state specificity, and chemical inhibition. Based on these structures, we review the structural chemistry of SET domain PMTs, and propose general concepts towards the development of selective inhibitors.

2011-08-22

415

Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)

A 269-kilobase conjugative plasmid, pK29, from a Klebsiella pneumoniae strain was sequenced. The plasmid harbors multiple antimicrobial resistance genes, including those encoding CMY-8...Full Text Available

2007-08-01

416

Environmental Research Database

BackgroundLand plant genomes contain multiple members of a eukaryote-specific gene family encoding proteins with pentatricopeptide repeat (PPR) motifs. Some PPR proteins were shown...Full Text Available

417

Plant Sulphur Nutrition

DescriptionThe recent sequencing of the complete genomes of several streptomycete species revealed the presence of a large number of cryptic' secondary metabolic gene clusters, and led to the realisation that these organisms have the ability to produce many more natural products than had previously been recognised. One of the aims of our work is to identify the physiological signals and regulatory mechanisms responsible for the activation of these 'cryptic' pathways, thus unleashing the full biosynthetic p [continued...

2010-01-31

418

Ordered Cloned DNA Map of the Genome of Vibrio cholerae 569B and Localization of Genetic Markers

Meta-Analysis for Genome-Wide Association Study Identifies Multiple Variants at the BIN1 Locus Associated with Late-Onset Alzheimer's Disease

By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol. 176:1105–1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic...Full Text Available

1998-02-01

419

Isolation, sequence, and expression in Escherichia coli of an unusual thioredoxin gene from the cyanobacterium Anabaena sp. strain PCC 7120.

Recent GWAS studies focused on uncovering novel genetic loci related to AD have revealed associations with variants near CLU, CR1, PICALM and BIN1....Full Text Available

420

Investigation of genomic instability by assay of DNA fingerprint from the offspring of male mice exposed to chronic low-level #gamma#-radiation

Two sequences with homology to a thioredoxin oligonucleotide probe were detected by Southern blot analysis of Anabaena sp. strain PCC 7120 genomic DNA. One of the sequences was shown to code for a protein...Full Text Available

1989-01-01

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421

Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713

By polymerase chain reaction with arbitrary primer (AP-PCR), the possibility of transmission of genome instability to somatic cells of the offspring (F_1 generation) from male parents of mice exposed to chronic low-dose #gamma#-radiation was studied. Male mice 15 days after exposure to 10-50 cGy were mated with unirradiated females. Biopsies were taken from tale tips of two month-old mice progeny for DNA separation. Primer in the AP-PCR was 20-mer oligonucleotide flanking the micro-satellite locus Atplb2 on chromosome 11 of the mouse. Comparative analysis of individual fingerprints of AP-PCR products on DNA-templates from the offspring of irradiated and unirradiated male mice revealed an increased variability of micro-satellite-associated sequences in the genome of the offspring of males exposed to 25 and 50 cGy. DNA-fingerprints of the offspring of male mice exposed to chronic irradiation doses 10 and 25 cGy. 15 days before fertilization (at ...

2000-11-20

422

Human thrombomodulin gene is intron depleted: nucleic acid sequences of the cDNA and gene predict protein structure and suggest sites of regulatory control.

Mycoreovirus 1 (MyRV1), a member of the Reoviridae family possessing a genome consisting of 11 dsRNA segments (S1–S11), and the prototype hypovirus (CHV1-EP713)...Full Text Available

2008-12-01

423

Haplotyping and copy number estimation of the highly polymorphic human beta-defensin locus on 8p23 by 454 amplicon sequencing

We have isolated a human thrombomodulin cDNA, and a human genomic clone containing the putative promoter domain, as well as the translated and untranslated regions of the endothelial cell receptor....Full Text Available

1987-09-01

424

Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation

BackgroundThe beta-defensin gene cluster (DEFB) at chromosome 8p23.1 is one of the most copy number (CN) variable regions of the human genome. Whereas individual DEFB CNs have been...Full Text Available

425

Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene.

BackgroundEstrogen receptors alpha (ERα) and beta (ERβ) are transcription factors (TFs) that mediate estrogen signaling and define the hormone-responsive phenotype...Full Text Available

426

Genome-wide analysis reveals rapid and dynamic changes in miRNA and siRNA sequence and expression during ovule and fiber development in allotetraploid cotton (Gossypium hirsutum L.)

We have isolated the human prointerleukin 1 (proIL-1) beta gene from leukocyte and fetal liver libraries. The nucleotide sequence and its gene organization reveals that the proIL-1 beta gene is composed...Full Text Available

1986-10-24

427

Genome-wide analysis of N¹-methyl-adenosine modification in human tRNAs

BackgroundCotton fiber development undergoes rapid and dynamic changes in a single cell type, from fiber initiation, elongation, primary and secondary wall biosynthesis, to fiber...Full Text Available

2009-01-01

428

Genome analysis of F. nucleatum sub spp vincentii and its comparison with the genome of F. nucleatum ATCC 25586.

The N¹-methyl-Adenosine (m¹A58) modification at the conserved nucleotide 58 in the TΨC loop is present in most eukaryotic tRNAs. In yeast, m¹A58 modification...Full Text Available

2010-07-01

429

Genome Sequence of a Lancefield Group C Streptococcus zooepidemicus Strain Causing Epidemic Nephritis: New Information about an Old Disease

We present the draft genome sequence and its analysis for Fusobacterium nucleatum sub spp. vincentii (FNV), and compare that genome with F. nucleatum ATCC 25586 (FN). A total of 441 FNV open reading frames (ORFs) with no orthologs in FN have been identified. Of these, 118 ORFs have no known function and are unique to FNV, whereas 323 ORFs have functional orthologs in other organisms. In addition to the excretion of butyrate, H2S and ammonia-like FN, FNV has the additional capability to excrete lactate and aminobutyrate. Unlike FN, FNV is likely to incorporate galactopyranose, galacturonate, and sialic acid into its O-antigen. It appears to transport ferrous iron by an anaerobic ferrous transporter. Genes for eukaryotic type serine/threonine kinase and phosphatase, transpeptidase E-transglycosylase Pbp1A are found in FNV but not in FN. Unique ABC transporters, cryptic phages, and three types of restriction-modification systems have been ...

2003-06-01

430

Explore - The Lab - Australian Broadcasting Corporation's Gateway to Science

Outbreaks of disease attributable to human error or natural causes can provide unique opportunities to gain new information about host-pathogen interactions and new leads for pathogenesis research....Full Text Available

431

Evolution of a cluster of innate immune genes (?-defensins) along the ancestral lines of chicken and zebra finch

...Explore - The Lab - Australian Broadcasting Corporation's Gateway to Science Radio TV Shop News Sport Local Children Science Environment more Topics help Science Home News in Science Features Explore TV & Radio Dr Karl Play Podcasts DNA - The discovery of the double helix structure of DNA completely changed biology and opened up the new field of genetics. The 'backbone of life' has given us the human genome, stem cell research, ...

432

Environmentally-Induced Malignancies: An In Vivo Model to Evaluate the Health Impact of Chemicals in Mixed Waste

BackgroundAvian β-defensins (AvBDs) represent a group of innate immune genes with broad antimicrobial activity. Within the chicken genome, previous work identified 14 AvBDs...Full Text Available

433

Dramatic Effects of 2-Bromo-5,6-Dichloro-1-?-d-Ribofuranosyl Benzimidazole Riboside on the Genome Structure, Packaging, and Egress of Guinea Pig Cytomegalovirus

Occupational and environmental exposure to organic ligands, solvents, fuel hydrocarbons, and polychlorinated biphenyls are linked with increased risk of hematologic malignancies. DOE facilities and waste sites in the U.S. are contaminated with mixtures of potentially hazardous chemicals such as metals, organic ligands, solvents, fuel hydrocarbons, polychlorinated biphenyls and radioactive isotopes. A major goal of this project was to establish linkage between chemical/radiation exposure and induction of genomic damage in target populations with the capability to undergo transformation.

2001-05-04

434

Differential decay of parent-of-origin-specific genomic sharing in cystic fibrosis-affected sib pairs maps a paternally imprinted locus to 7q34

The halogenated benzimidazoles BDCRB (2-bromo-5,6-dichloro-1-β-d-riborfuranosyl benzimidazole riboside) and TCRB (2,5,6-trichloro-1-β-d-riborfuranosyl benzimidazole...Full Text Available

2004-02-01

435

Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels

Cystic fibrosis (CF) is a monogenic disease characterized by a high variability of disease severity and outcome that points to the role of environmental factors and modulating genes that shape the course...Full Text Available

2010-05-01

436

DNA rearrangements from #gamma#-irradiated normal human fibroblasts preferentially occur in transcribed regions of the genome

Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived...Full Text Available

2009-01-01

437

Computational identification of developmental enhancers:conservation and function of transcription factor binding-site clustersin drosophila melanogaster and drosophila psedoobscura

Full text: DNA rearrangement events leading to chromosomal aberrations are central to ionizing radiation-induced cell death. Although DNA double-strand breaks are probably the lesion that initiates formation of chromosomal aberrations, little is understood about the molecular mechanisms that generate and modulate DNA rearrangement. Examination of the sequences that flank sites of DNA rearrangement may provide information regarding the processes and enzymes involved in rearrangement events. Accordingly, we developed a method using inverse PCR that allows the detection and sequencing of putative radiation-induced DNA rearrangements in defined regions of the human genome. The method can detect single copies of a rearrangement event that has occurred in a particular region of the genome and, therefore, DNA rearrangement detection does not require survival and continued multiplication of the affected cell. Ionizing radiation-induced DNA ...

2003-08-17

438

Comparative transcriptional pathway bioinformatic analysis of dietary restriction, Sir2, p53 and resveratrol life span extension in Drosophila

Background The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. Results We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated to those of neighboring genes; the ...

2004-08-06

439

A multiple comparison approach using whole genome transcriptional arrays was used to identify genes and pathways involved in calorie restriction/dietary restriction (DR) life span extension in Drosophila....Full Text Available

2011-03-15

440

Biochemical and Structural Characterization of the Secreted Chorismate Mutase (Rv1885c) from Mycobacterium tuberculosis H₃₇R_v: an *AroQ Enzyme Not Regulated by the Aromatic Amino Acids

The state of chromatin during the cell cycle was examined using synchronized cultures of CHO hamster cells. Results support Mazia's dynamic chromosome cycle model and indicate that DNA-interactive chemotherapeutic agents elicit different types of kinetic responses in treated cells, suggesting a degree of specificity of interaction between various alkylating and intercalating agents and the genome. Effects of sarkosyl crystals, heparin, and chemotherapeutic agents, neocarzinostatin and adriamycin, on chromation are discussed. (HLW)

1976-01-01

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441

Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.

The gene Rv1885c from the genome of Mycobacterium tuberculosis H₃₇R_v encodes a monofunctional and secreted chorismate mutase (*MtCM) with a 33-amino-acid...Full Text Available

2006-12-01

442

Analysis of the 10q11 Cancer Risk Locus Implicates MSMB and NCOA4 in Human Prostate Tumorigenesis

The Epstein-Barr virus nuclear antigen (EBNA I) present in latently infected cells is encoded in a 2-kilobase exon contained in the BamHI K viral genomic fragment. This exon is, however, found within...Full Text Available

1985-12-01

443

A Nonparametric Mean-Variance Smoothing Method to Assess Arabidopsis Cold Stress Transcriptional Regulator CBF2 Overexpression Microarray Data

Genome-wide association studies (GWAS) have established a variant, rs10993994, on chromosome 10q11 as being associated with prostate cancer risk. Since the variant is located outside of a protein-coding...Full Text Available

2010-11-01

444

Yakima River Species Interactions Studies, Annual Report 1999.

Microarray is a powerful tool for genome-wide gene expression analysis. In microarray expression data, often mean and variance have certain relationships. We present a non-parametric mean-variance smoothing...Full Text Available

445

Radiological and Environmental Research Division: ecology. Annual report, January-December 1982

Species interactions research and monitoring was initiated in 1989 to investigate ecological interactions among fish in response to proposed supplementation of salmon and steelhead in the upper Yakima River basin. This is the eighth of a series of progress reports that address species interactions research and pre-supplementation monitoring of fishes in the Yakima River basin. Data have been collected prior to supplementation to characterize the ecology and demographics of non-target taxa (NTT) and target taxon, and develop methods to monitor interactions and supplementation success. Major topics of this report are associated with implementing NTT monitoring prescriptions for detecting potential impacts of hatchery supplementation, hatchery fish interactions, and monitoring fish predation indices. This report is organized into four chapters, with a general introduction preceding the first chapter. This annual report summarizes data collected ...

2001-06-01

446

Methods and results of an evaluation of aquatic receptor risk at the Rocky Flats Environmental Technology Site, Golden, Colorado

This is the annual report of the Radiological and Environmental Division of the Argonne National Laboratory for 1982. Studies of the effects of ozone on crop growth and yield have been carried out by the Terrestrial Ecology Group for winter wheat and for sorghum. The Microcosms for Acid Rain Studies (MARS) facility was completed in the early summer. Controlled investigations of plant and soil responses in acid rain were initiated with crop plants grown in two different midwestern soil types. The Transuranics Group has found that the solubility and adsorptive behavior of plutonium previously observed at fallout concentrations in natural waters (approx. 10/sup -16/ to 10/sup -18/ M) is applicable at plutonium concentrations as high as 10/sup -8/ M. The Lake Michigan eutrophication model has been adapted to operation in a Monte Carlo mode. Simulations based on yearly phosphorus loadings and winter conditions were selected at random from prescribed probability ...

1983-09-01

447

An evaluation of the ecological consequences of partial-power operation of the K Reactor, SRS

The Rocky Flats Environmental Technology Site (RFETS) has historically released radionuclide chemicals of potential concern into the surrounding environment. The off-site environment was evaluated for Pu"2"3"9"/"2"4"0 and Am"2"4"1 occurrence. An evaluation of exposure and effects to the aquatic ecology within off-site areas including: Standley Lake, Great Western Reservoir, Mower Reservoir and portions of Big Dry Creek, Walnut Creek, and Woman Creek was performed for the completion of an Ecological Risk Assessment. Collocated sampling activities were performed for surface water, sediment, benthic macroinvertebrates and fish. Results of the analytical data were used to assess ongoing exposure and effects. Data collected to determine effects (chemical content of fish tissue, diversity and density of macroinvertebrate populations) provided some of the necessary information needed to evaluate risk. However, due to conditions of interfering stressor ...

1995-11-05

448

An Ecological Risk Model for Early Childhood Anxiety: The Importance of Early Child Symptoms and Temperament

The K Reactor at the Savannah River Site (SRS) shut-down in spring 1988 for maintenance and safety upgrades. Since that time the receiving stream for thermal effluent, Indian Grave Branch and Pen Branch, have undergone a pattern of post-thermal recovery that is typical of other SRS streams following removal of thermal stress. Divesity of fish and aquatic macroinvertebrate communities has increased and available habitats have been colonized by numerous species of herbaceous and woody plants. K Reactor is scheduled to resume operation in 1991 and operate through 1992 without a cooling tower to cool the discharge. It is likely that the reactor will operate at approximately one-third to one-half of full power (800--1200 MW thermal) during this period and effluent temperatures will be substantially lower than earlier operation at full power. Monthly average discharge temperatures at half-power operation will range from approximately 42 degrees C in winter to 49 degrees C in summer. The ...

449

A hierarchical framework for coupling surface fluxes to atompsheric general circulation models: The homogeneity test

Childhood anxiety is impairing and associated with later emotional disorders. Studying risk factors for child anxiety may allow earlier identification of at-risk children for prevention efforts. This study applied an ecological risk model to address how early childhood anxiety symptoms, child temperament, maternal anxiety and depression symptoms, violence exposure, and sociodemographic risk factors predict school-aged anxiety symptoms. This longitudinal, prospective study was conducted in a representative birth cohort (n = 1109). Structural equation modeling was used to examine hypothesized associations between risk factors measured in toddlerhood/preschool (age = 3.0 years) and anxiety symptoms measured in kindergarten (age = 6.0 years) and second grade (age = 8.0 years). Early child risk factors (anxiety symptoms and temperament) emerged as the most robust predictor for both parent-and child-reported anxiety outcomes and mediated the effects of maternal and ...

2011-05-01

450

A framework for assessing relative risks associated with multiple stressors in Port Valdez, Alaska

The atmosphere and the biosphere are inherently coupled to one another. Atmospheric surface state variables such as temperature, winds, water vapor, precipitation, and radiation control biophysical, biogeochemical, and ecological processes at the surface and subsurface. At the same time, surface fluxes of momentum, moisture, heat, and trace gases act as time-dependent boundary conditions providing feedback on atmospheric processes. To understand such phenomena, a coupled set of interactive models is required. Costs are still prohibitive for computing surface/subsurface fluxes directly for medium-resolution atmospheric general circulation models (AGCMs), but a technique has been developed for testing large-scale homogeneity and accessing surface parameterizations and models to reduce this computational cost and maintain accuracy. This modeling system potentially bridges the observed spatial and temporal ranges yet allows the incorporation of necessary details about ...

1993-12-31

451

A discussion of the development of sandy land from the viewpoint of ecology

The purpose of this assessment is to develop a versatile process that will provide a mechanism for evaluating both present and future risks to this environment. Much of the regulatory and environmental interest in the port has centered around a Ballast Water Treatment facility that treats and discharges up to 30 mgd of oily ballast water brought in by crude oil tankers. However, six point discharges and other potential sources of pollution exist in the area. The authors have delineated eleven subareas in the port in order to identify the potential anthropogenic stressors, as well as the receptors that could be exposed to these stressors. Potential effects were then characterized for each exposure. Each component is ranked and integrated, resulting in a relative risk estimate in each subarea. Both the discernible risks, based on available data, and the data gaps are presented. Uncertainty is expressed as a range of high and low risk associated with each component. Results of the ranking ...

1995-12-31

452

[Changes in ecological features of soils after controlled fires in forests defoliated by the Siberian moth in the southern taiga subzone of the Enise? Region, Siberia].

This article discusses the proper use of sandy land in China from the viewpoint of ecology. The many low-yield fields in every locality across China include sandy land that is unsuited to the cultivation of grains such as paddy rice, corn and wheat. Separate investigations of the northern plain and the southern coast between 1980 and 1982 demonstrated that sandy land in a warm climatic zone (e.g. Huang He) is suited to peanuts, soybeans and other oil-bearing crops; that forestation can be carried out on sandy land in Inner Mongolia and Xinjiang; and that coastal sandy land has much salinity and is best suited to growing horsetail beefwood. Moreover, the creation of windbreaks along the coasts of southern China has lessened the threat of wind-blown sand which had made rice not worth cultivating on sandy land. It is concluded that different crops can be grown on the sandy soil of China's temperate, warm, semitropical and tropical zones.

1983-01-01

453

Data on the postfire dynamics of soil properties in the foci of Siberian moth population outbreaks are considered. It has been shown that controlled fires set in pest-defoliated forests result in the loss of approximately 75% of carbon and 50% of nitrogen from the forest litter through their emission into the atmosphere and in the enrichment of the upper soil horizons with potassium and phosphorus (this concerns both total and movable forms). Microbiological processes in the organogenic horizon undergo significant transformation, the density of microarthropods decreases, and the abundance of mites becomes hundreds of times lower. PMID:15354965

454

Wetlands stewardship

The use of the isotope "3"2P in the study of some ecological aspects of a laboratory stream ecosystem

Wetlands have important ecological values and functions. It is estimated that 80 percent of the Nation's coastal fisheries are dependent on wetlands for spawning, nursery areas, and food sources. Both coastal and inland wetlands provide essential breeding, nesting, feeding, and predator escape habitats for millions of waterfowl, other birds, mammals, and reptiles. Well over one-third of the 564 plant and animal species listed as threatened or endangered in the United States utilize wetland habitats during some portion of their life cycle. Wetlands Stewardship is intended as a resource for everyone interested in wetlands protection.

1992-04-01

455

The river Elbe. A case study for the ecological and economical chain of sediments

A laboratory stream consisting of two stream sections interrupted by two pools was filled with a mixture of tap water and organically enriched water from rivers nearby. Bottom sediment material, Potamogeton pectinatus, macro-invertebrate organisms, as well as the mosquito fish, Aplocheilichthys johnstonii were collected from rivers around Johannesburg and introduced into the laboratory stream. After initial acclimatization, the distribution of the isotope "3"2P through this laboratory stream was followed. Absorption of this isotope by benthic algae, Potamogeton pectinatus, several macro-invertebrate organisms as well as Aplocheilichthys johnstonii was recorded.

456

Relationship between copper speciation and cyanobacterial distribution in harbors and other coastal environments. Final report, 1 July 1993--1 November 1998

Industrial activities in the river basin of the Elbe have a very long tradition, and have been resulting in the contamination of sediments for centuries. Contamination lasted until the fall of the iron curtain; since then, the situation has improved significantly. In the transition zone between freshwater systems and the marine environment, ports like Hamburg still have to bear this burden of history. An overall (contaminated) management strategy should be developed in the context of the European Water Framework Directive with the emphasis on source control. (orig.)

2002-07-01

457

Progress of the BT-EdF-CEA project. The lithium polymer battery; Avancees du projet BT-EdF-CEA. Batterie lithium polymere

The long term goal is to determine the ecological importance of specific toxic metals (copper and zinc) in harbors, through an understanding of their chemistry and biological effects at the base of marine food chains. This work provides information on potential problems with Zn and Cu, which may help to foresee problems in the future. Anthropogenic inputs of both metals are increasing; they are derived from diverse sources and are a simple function of population growth in coastal regions.

1998-11-01

458

Proceedings of the Canadian Society for Bioengineering CSBE/SCGAB 2009 annual general meeting and technical conference

The lithium-polymer energy storage technology requires the production of thin films of huge surface. The BT-EdF-CEA consortium has studied the various manufacturing techniques of these films and their assembly. The process was chosen according to its productivity, low expensiveness, ecological impact and energy performances with capacities reaching 40 Ah. This paper explains: the objectives and specifications of the project, the advantage of the consortium and the role of the different partners, the results (coating, dry extrusion and battery element manufacturing techniques), and the electrochemical performances of the elements. (J.S.)

1996-12-31

459

Land planarians (Platyhelminthes: Tricladida: Geoplanidae) from the Iberian Peninsula: new records and description of two new species, with a discussion on ecology

This conference was attended by experts on current issues in engineering of an urban ecosystem, composting, biofuels and green energy. The meeting provided a forum to discuss advances in the application of engineering principles and practices dealing with biological systems for the production of food, bioproducts and energy. The discussions focused on engineering practices in agriculture, forestry, bioresources, biochemistry and biosystems. The 7 technical sessions of the conference were entitled: aquaculture; safety and training; bioprocessing; energy production and biofuels; environment and ecology; soil and water; and development of technologies. The conference feature 58 presentations, of which 9 have been catalogued separately for inclusion in this database. refs., tabs., figs.

2009-07-01

460

British Library Electronic Table of Contents (United Kingdom)

Two new species of the genus Microplana are described from the Iberian Peninsula. The new taxa are compared with congeneric species. Distributional records for two other European species, Microplana monacensis (Heinzel, 1929) and Microplana groga Jones et al. 2008, are presented and the presence of Microplana terrestris (Muller, 1774) is confirmed on the Iberian Peninsula. A partial re-description of Microplana nana Mateos, Giribet and Carranza, 1998 is provided. The finding of a new and probably introduced, but unidentified, species of land planarian is reported. Land planarians were generally found in the vicinity of deciduous trees and rivers.

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Information on Hanford site cribs and septic systems

Genotoxic damage in polychaetes: A study of species and cell-type sensitivities

This document provides information on septic systems with a design capacity of greater than 14,500 gal/d and cribs submitted as requested by the Washington State Department of Ecology. Data for this submittal were taken from the Waste Information Database System (WIDS) and the Hanford Environmental Compliance Record (HECR) database. The current definition used in WIDS for an ''inactive facility'' is one that either no longer receives waste or plans to in the future. Information concerning the deactivation method for a facility is included when such information is available.

1988-05-01

462

British Library Electronic Table of Contents (United Kingdom)

The marine environment is becoming increasingly contaminated by environmental pollutants with the potential to damage DNA, with marine sediments acting as a sink for many of these contaminants. Understanding genotoxic responses in sediment-dwelling marine organisms, such as polychaetes, is therefore of increasing importance. This study is an exploration of species-specific and cell-specific differences in cell sensitivities to DNA-damaging agents in polychaete worms, aimed at increasing fundamental knowledge of their responses to genotoxic damage. The sensitivities of coelomocytes from three polychaetes species of high ecological relevance, i.e. the lugworm Arenicola marina, the harbour ragworm Nereis diversicolor and the king ragworm Nereis virens to genotoxic damage are compared, and dif...

2008-01-01

463

Forum: Science and Innovation for Sustainable Development - Opportunities

Environmental sciences and applications. Volume 4. Strategy for the ozone layer

...assistantship in Suburban Ecology Location: Bedford, NY Deadline: August 15, 2008 Postdoctoral Fellow in energy Location: University of Waterloo, Ontario, Canada Deadline: August 11, 2008 Junior Professional Fellowship Sustainable Development Governance Programme Location: UNU, Yokohama, Japan Deadline: August 1, 2008 Center for Ocean Solutions Early Career Fellowship Program Location: Stanford, California Deadline: July 15, 2008 Asian MetaCentre for Population and Sustainable Development Analysis Postdoctoral Research Fellow Location: Singapore Deadline: July 15, ...

464

Environmental restoration remedial action quality assurance requirements document

A synthesis of papers based on the United Nations Environment Programme meeting on the ozone layer, Washington DC, this book contains valuable information on ongoing and planned activities concerned with stratospheric ozone problems, and presents the recommendations for further action resulting from the meeting. Possible changes in the characteristics of the ozone layer are discussed, together with the environmental, ecological, climatic, economic, and health implications of stratospheric ozone depletion. A comprehensive survey of current research in five European countries, the USA, Canada, and Australia is included and the volume is concluded by the UNEP report of the meeting and a world plan of action.

1980-01-01

465

Electric cars: No-noise and low-pollution. Elektroautos. Laermfrei und schadstoffarm

The environmental Restoration Remedial Action Quality Assurance Requirements Document (DOE/RL 90-28) defines the quality assurance program requirements for the US Department of Energy-Richland Field Office Environmental Restoration Remedial Action Program at the Hanford Site, Richland, Washington. This paper describes the objectives outlined in DOE/RL 90-28. The Environmental Restoration Remedial Action Program implements significant commitments made by the US Department of Energy in the Hanford Federal Facility Agreement and Consent Order entered into with the Washington State Department of Ecology and the US Environmental Protection Agency.

1991-09-08

466

Comprehensive view of BMFT-funded research on forest decline. Gesamtdarstellung der oeffentlich gefoerderten Forschung ueber Waldschaeden

Growing ecological awareness in the car industry resulted in further developments of the otto and diesel engines, and of alternative fuels like cars with electric drive. This article gives an overview on a number of pertinent developments. The possible uses of electric cars are still limited by the battery in terms of storage capacity, output, service life and weight so that for the time being their use can be expected to be restricted to public and local-authority antreprises or to companies driving within city areas preferably. A break-through is expected by the industry in the field of high-temperature batteries although there are still a number of problems unsolved. (BWI).

1991-01-01

467

CSIRO PUBLISHING - Wildlife Research

Since 1983, about 600 research projects received a total of DM 250 million public funds for the following tasks: - Development of scientific fundamentals for monitoring and observation of forest decline and its ecological and economic consequences. - Investigation of the cause-effect relationship of forest damage, with particular regard to the involvement of the various compartments of the forest ecosystem and to the contribution of different pollutants. - Development of the scientific fundamentals required for forestry measures to accompany the air pollution abatement measures. The investigations comprised both laboratory and field tests. The findings and conclusion are summarized. (orig./MG).

1988-12-01

468

Atomic power of Germany and ecology

... Cameron; Lowell A. Miller; Miller, Lowell A. CSIRO PUBLISHING - Wildlife Research Books Journals Multimedia About Us Shopping Cart You are here: Journals Wildlife Research Ecology, Management and Conservation in Natural and Modified Habitats Search ... Cameron and Lowell A. Miller Abstract Context. Contraception is increasingly used as a management technique to reduce fertility in wildlife populations; however, the feasibility of contraceptive formulations has been limited until recently because they have required multiple treatments to achieve prolonged infertility. ...

469

An application of the analysis of variance of measures repeated in an experiment with heavy metals

The NPPs safety system in Germany is discussed. It is shown that there exists no threat for the German NPPs at the peace times. They release insignificant quantities of radioactive substances into the water and atmosphere. The average equivalent dose constitutes 0.0005 mSv annually. The annual equivalent dose for the personnel is equal to 4.4 mSv. At the same time, the NPPs contribute to a certain degree to the environmental medium improvement, preventing the ingress therein of the sulfur and carbon dioxide, dust and nitrogen oxides by application of fossil fuels. Attention is also paid to reprocessing facilities and also to the nuclear fuel wastes disposal. The advantages of the nuclear power engineering in comparison with the fossil fuel power engineering are enumerated

470

Local chromatin structure of heterochromatin regulates repeatedDNA stability, nucleolus structure, and genome integrity

A revision of some basic concepts related to the analysis of variance of repeated measures is presented within an ecological context topics such as the types of experiments in which the technique is applicable, the hypotheses of interest, and its preference over other traditional techniques such as regression and conventional analysis of variance, are discussed. As an example, the technique was successfully applied to an experiment carried out at Cienaga Grande de Santa Marta, Colombia, in which the concentration of cadmium #mu#g/g in leaves of the black mangrove Avicennia germinans was measured in several monitoring stations and throughout several sampling intervals representing seasons.

1997-11-01

471

The off-gas cleaning system for gases formed during the vitrification of high-activity wastes from the nuclear power plant A 1

Heterochromatin constitutes a significant portion of the genome in higher eukaryotes; approximately 30% in Drosophila and human. Heterochromatin contains a high repeat DNA content and a low density of protein-encoding genes. In contrast, euchromatin is composed mostly of unique sequences and contains the majority of single-copy genes. Genetic and cytological studies demonstrated that heterochromatin exhibits regulatory roles in chromosome organization, centromere function and telomere protection. As an epigenetically regulated structure, heterochromatin formation is not defined by any DNA sequence consensus. Heterochromatin is characterized by its association with nucleosomes containing methylated-lysine 9 of histone H3 (H3K9me), heterochromatin protein 1 (HP1) that binds H3K9me, and Su(var)3-9, which methylates H3K9 and binds HP1. Heterochromatin formation and functions are influenced by HP1, Su(var)3-9, and the RNA interference (RNAi) pathway. My thesis project ...

2007-05-05

472

Structure of an accelerator island with one step on double period of conserving area mapping

A multielement apparatus for the removal of aerosols formed in the vitrification of simulated high-activity accident wastes from the decommissioned nuclear power plant A-1 has been designed, manufactured and tested. The apparatus consists of two cooling and condensing steps connected in series, and of five filtration steps also connected in series. The five filtration steps allowed to achieve a decontamination factor of 1x10"5 in the case of removal of a NaCl("2"4Na) testing model aerosol. The decontamination factor for removal of the cesium aerosol by the whole apparatus was 3.3x10"6. In the first filtration step the aerosols are removed by the cartridge with a filtration fabric made of polyethylene fibers, in the second step by the cartridge with a granulated natural material, in the third step by the cartridge with glass-fiber filtration paper, in the fourth ...

473

Self installable: floating production facility requires only light support craft

The structure of the P-2, S-1 accelerator mode with one step, S-1, on double period, P-2 for systems, described by the Harper mapping as well as by standard mapping, is analyzed. Detailed analysis of stability criteria is given and comparison with the results of numerical analysis is performed. Three-period compression of the P-2, S-1 island is revealed in the standard mapping. Refs. 7, figs. 7.

474

PhET Teacher Ideas and Activities: Images from Convex Lenses

An integrated multiwell floating production, storage, and offloading (FPSO) facility was successfully installed at the Tazerka field offshore Tunisia in 1982. Because there is good reason to believe that the installation techniques developed for the Tazerka FPSO can be applied to even larger systems in deeper waters, this paper presents the design and step-by-step installation procedures developed at Tazerka.

1983-05-01

475

Conclusions from the last five years of experiments in the field of food irradiation in Hungary

This is an inquiry-based module created specifically for use with the PhET simulation Geometric Optics. It includes a lesson plan for teaching about convex lenses, step-by-step student directions, and a set of Power Point "clicker" questions for use as warm-up questions or informal assessment. This resource is part of a larger collection of interactive Java simulations for students of physics, developed by the Physics Education Technology project at the University of Colorado.

2009-05-20

476

Submission to the Georges Bank Review Panel

AGROSTER Co irradiates food packaging material and some types of spices for the meat industry. The step by step strategy of AGROSTER has been very successful. In the last year, its gamma facility was used as much as possible to irradiate food items. The government of Hungary has recognized the benefit of this technology and has given financial assistance to establish a large commercial gamma irradiator in Budapest.

1988-01-01

477

Ecological risks associated with the application of sewage sludge to non-agricultural ecosystems

The Georges Bank, off southwestern Nova Scotia, is one of the most biologically productive continental shelf ecosystems in the world. It supports a wide diversity of species, including many seabirds. In 1988, federal and provincial legislation placed a moratorium on petroleum exploration and drilling on the Georges Bank, directing the ministers of Natural Resources Canada, and of the Nova Scotia Department of Natural Resources to make a decision regarding the moratorium by January 1, 2000. This report by the Ecology Action Centre (EAC) asks the Georges Bank Review Panel to recommend extending the moratorium on Georges Bank and adjacent areas until 2012, in part to match the American moratorium. Other reasons why the panel made this recommendation were explored. The principal reason is the EAC concern that when the moratorium is lifted, several oil and gas developments will proceed in this ecologically significant offshore area that could result ...

1999-01-01

478

Screen-printed Emitter-Wrap-Through solar cell with single step side selective emitter with 18.8% efficiency

The Clean Water Act of 1977 directed EPA to establish standards for use and disposal of sewage sludge (biosolids). The application of biosolids to non-agricultural lands is becoming increasingly important as a method of waste disposal. Ecological endpoints at the population, community, and/or ecosystem level have not previously been emphasized in the development of regulatory standards for municipal sewage sludge. This risk assessment focuses on terrestrial endpoints in four ecosystem types to which substantial quantities of sludge have been applied or are expected to be applied in the future: northwest Douglas-fir forest, southeastern loblolly pine plantation, eastern deciduous forest, and semi-arid rangeland. Conceptual models suitable for all ecosystems were developed that depict the links among assessment endpoints. Estimates of risks to wildlife from contaminants and simulations of impacts of nitrogen in sewage sludge on the structure and function of forest ...

1995-12-31

479

British Library Electronic Table of Contents (United Kingdom)

Abstract A fabrication process for Emitter-Wrap-Through solar cells on monocrystalline material with high quality gap passivation by wet thermal silicon dioxide is investigated. Masking and structuring steps are performed by screen-printing technology. Via-holes are created by an industrially applicable high-speed laser drilling process. The cell structure features a selective emitter structure fabricated in a single high temperature step: a highly doped emitter at the via-holes and the rear side, allowing for a low via-hole resistivity as well as a low resistivity contact to screen-printed pastes, and a moderately doped front side emitter exhibiting high quantum efficiency in the low wavelength range. Therefore a novel approach is applied depositing either doped or undoped PECVD silicon d...

2011-01-01

480

Preparation of AgCl Nanoparticles@Ancient Textile with Antibacterial Activity under Ultrasound Irradiation

British Library Electronic Table of Contents (United Kingdom)

The growth of silver chloride nanoparticles on ancient textile was achieved by sequential dipping steps in alternating bath of potassium chloride and silver nitrate under ultrasound irradiation. The effect of ultrasound irradiation and sequential dipping steps in growth of the AgCl nanoparticles has been studied. These systems depicted a decrease in the particles size accompanying a decrease in the sequential dipping steps. The samples were characterized with powder X-ray diffraction (XRD) and scanning electron microscopy. XRD analyses indicated that the prepared AgCl nanoparticles on fiber were crystalline. The textile samples containing AgCl nanoparticles were tested for their antibacterial efficacy against Escherichia coli and were found to possess significant antibacterial activity.

2011-01-01

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481

Oxidative desulphurization study of gasoline and kerosene

British Library Electronic Table of Contents (United Kingdom)

Desulphurization of gasoline and kerosene was carried out using organic and inorganic oxidants. Among the organic oxidants used were hydrogen peroxide in combination with acetic acid, formic acid, benzoic acid and butyric acid, while inorganic oxidants used included potassium permanganate and sodium perchlorate. The oxidation of each petroleum oil was carried out in two steps; the first step consisted of oxidation of the feed at moderate temperature and atmospheric pressure while in the second step, the oxidized mixture was extracted with azeotropic mixture of acetonitrile-water. A maximum desulphurization has occurred with NaClO4 and hydrogen peroxide and acetic acid, which are 68% and 61%, respectively in case of gasoline and 66% and 63%, respectively in case of kerosene oil. The FTIR st...

2010-01-01

482

Mineral carbonation accelerated by dicarboxylic acids as a disposal process of carbon dioxide

British Library Electronic Table of Contents (United Kingdom)

Mineral carbonation is based on the reaction of carbon dioxide with metal-oxide bearing minerals, usually containing magnesium or calcium silicate, to form hardly soluble carbonates and other solid byproducts. The concept is based on acceleration of the naturally occurring rock weathering process. In the present work the calcium silicate is present in the mineral, wollastonite. To accelerate the process and make it potentially useful for practical applications, mineral carbonation is conducted here using an indirect two-step route in which the reactive component (Ca^2^+ ions in considered case) is first extracted from the mineral matrix and afterwards carbonated. Two solid byproducts are formed in this process: silica in the extraction step and calcium carbonate in the carbonation step. In...

2011-01-01

483

Measuring interesting rules in Characteristic rule

Heat loss from an open cavity

Finding interesting rule in the sixth strategy step about threshold control on generalized relations in attribute oriented induction, there is possibility to select candidate attribute for further generalization and merging of identical tuples until the number of tuples is no greater than the threshold value, as implemented in basic attribute oriented induction algorithm. At this strategy step there is possibility the number of tuples in final generalization result still greater than threshold value. In order to get the final generalization result which only small number of tuples and can be easy to transfer into simple logical formula, the seventh strategy step about rule transformation is evolved where there will be simplification by unioning or grouping the identical attribute. Our approach to measure interesting rule is opposite with heuristic measurement approach by Fudger and Hamilton where the more complex concept ...

2010-01-01

484

Gradiometry coexperiments to the gravity probe B and step missions

Cavity type receivers are used extensively in concentrating solar thermal energy collecting systems. The Solar Total Energy Project (STEP) in Shenandoah, Georgia is a large scale field test for the collection of solar thermal energy. The STEP experiment consists of a large field array of solar collectors used to supplement the process steam, cooling and other electrical power requirements of an adjacent knitwear manufacturing facility. The purpose of the tests, conducted for this study, was to isolate and quantify the radiative, conductive, and convective components of total heat loss, and to determine the effects of operating temperature, receiver angle, and aperture size on cavity heat loss. An analytical model for radiative heat loss was developed and compared with two other methods used to determine radiative heat loss. A proposed convective heat loss correlation, including effects of aperture size, receiver operating temperature, and ...

1995-12-01

485

Fuel injection control for diesel engine

The Gravity Probe-B (GP-B) spacecraft, designed to test predictions of general relativity, will fly in the mid 1990s. It will carry four electrostatically suspended gyroscopes in a cryogenic environment and will have a drag-free control system to minimize disturbances on the gyroscopes. The Stanford Test of Equivalence Principle (STEP) spacecraft, to fly later, will carry a set of test masses under very similar conditions. The possibility of using differential measurements of the GP-B gyroscopes suspension forces and the STEP tests mass displacement readout to form single-axis gravity gradiometers is explored. It is shown that the noise in the suspension systems is sufficiently small in the relevant frequency range, and that enough information is collected to compensate for the spacecrafts' attitude motion. Finally, using Breakwell's flat-earth approximation, these experiments are compared to other geodesy experiments and ...

1990-01-01

486

Formulation of multi-component explosives

A method is described for controlling fuel injection in a diesel engine having a fuel injection nozzle, comprising the steps of: detecting an idling state of the diesel engine and pressure in a combustion chamber; obtaining a pressure increment rate from the pressure detected by the detecting step; selecting a valve opening pressure of the fuel injection nozzle at a relatively low level when the idling state is detected by the detecting step, thereby setting a two peaks characteristics of a fuel injection rate; and controlling the fuel injection nozzle in response to the pressure increment rate so that the pressure increment rate in the combustion chamber is maintained within a predetermined value by changing a fuel injection quantity of a pilot injection from the fuel injection nozzle.

1987-11-10

487

Evaluation of chips quality by the analysis of two different harvesting methodologies

In a method for formulating a multi-component explosive composition, the steps are described comprising: (a) providing a defined body of unconsolidated particulate aluminum fuel granules having an average particle size within the range of 1/64-1/4 inch and an average bulk density within the range of 0.2-1.0 gm/cc; (b) providing a phase oxidizing liquid; and (c) flooding said body of particulate aluminum fuel of step (a) with the oxidizing liquid of step (b) to provide a bulk density of said mixture of oxidizing liquid and particulate aluminum fuel mass within the range of 1.2-1.7 gm/cc and provide an explosive formulation detonable at 20 C in a diameter of 4 inches by a one pound pentolite booster.

1993-07-13

488

Core-shell polymer nanorods by a two-step template wetting process

The Council for Research in Agriculture, Agricultural Engineering Research Unit (CRA-ING) in Rome, Italy has developed an innovative short-rotation forestry (SRF) harvesting system. The system involves a 2 step operation, notably the tree felling and inter-row windrowing performed by a feller windrower equipment; and subsequent chipping performed by a harvester equipped with a pick-up device. The low moisture content of the windrowed trees at harvesting time affects their physical qualities and mechanical strength throughout the chipping operation. The purpose of this study was to analyze the moisture losses of windrowed trees, in relation to the windrow location, on field storage and weather condition. In addition, the study characterized chips quality changes during on field storage by the 2 different harvesting systems. The innovative 2-step system was compared with the traditional 1-step harvesting system.

2010-07-01

489

A hybrid approach based on SERVQUAL and fuzzy TOPSIS for evaluating transportation service quality

One-dimensional core-shell polymer nanowires offer many advantages and great potential for many different applications. In this paper we introduce a highly versatile two-step template wetting process to fabricate two-component core-shell polymer nanowires with controllable shell thickness. PLLA and PMMA were chosen as model polymers to demonstrate the feasibility of this process. Solution wetting with different concentrations of polymer solutions was used to fabricate the shell layer and melt wetting was used to fill the shell with the core polymer. The shell thickness was analyzed as a function of the polymer solution concentration and viscosity, and the core-shell morphology was observed with TEM. This paper demonstrates the feasibility of fabricating polymer core-shell nanostructures using our two-step template wetting process and opens the arena for optimization and future experiments with polymers that are desirable for specific ...

2009-07-22

490

British Library Electronic Table of Contents (United Kingdom)

Managing service quality is vital to retain customer satisfaction and augment revenues for any business organization. Often it is difficult to assess service quality due to lack of quantifiable measures and limited data. In this paper, we present a hybrid approach based on SERVQUAL and fuzzy TOPSIS for evaluating service quality of urban transportation systems. The proposed approach consists of three steps. The first step involves development of a SERVQUAL based questionnaire to collect data for measuring transportation service quality. The participants provide linguistic assessments to rate the service quality criteria and the alternatives. In step 2, the linguistic ratings are combined through fuzzy TOPSIS to generate an overall performance score for each alternative. The alternative wit...

2011-01-01

491

Supercritical fluid extraction from dried banana peel (Musa spp., genomic group AAB): Extraction yield, mathematical modeling, economical analysis and phase equilibria

British Library Electronic Table of Contents (United Kingdom)

Supercritical fluid extraction from dried banana peel (Musa spp., subgroup Prata, genomic group AAB, popularly known in Brazil as Enxerto) was studied. The aspects investigated were: overall extraction curve (OEC), mass transfer modeling of the yield curves, economical analysis of the process and phase equilibrium data for the pseudo-ternary system of banana peel extract, carbon dioxide and ethanol. The extraction operating conditions evaluated were: pressure ranging from 100bar to 300bar, temperature from 40 to 50^oC and constant solvent flow rate of 5.0gCO2/min. Experimental extraction data were correlated using three kinetic models based on mass transfer equations (logistic, diffusion and Esquivel models). Phase equilibrium measurements were performed using pressure from 64.9bar to 239....

2010-01-01

492

Molecular cloning, genomic organization, and chromosomal localization of the human pancreatitis-associated protein (PAP) gene

Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32. 1

Pancreatitis-associated protein (PAP) is a secretory pancreatic protein present in small amounts in normal pancreas and overexpressed during the acute phase of pancreatitis. In this paper, the authors describe the cloning, characterization, and chromosomal mapping of the human PAP gene. The gene spans 2748 bp and contains six exons interrupted by five introns. The gene has a typical promoter containing the sequences TATAAA and CCAAT 28 and 52 bp upstream of the cap site, respectively. They found striking similarities in genomic organization as well as in the promoter sequences between the human and rat PAP genes. The human PAP gene was mapped to chromosome 2p12 using rodent-human hybrid cells and in situ chromosomal hybridization. This localization coincides with that of the reg/lithostathine gene, which encodes a pancreatic secretory protein structurally related to PAP, suggesting that both genes derived from the same ancestral gene by duplication. 35 refs., 4 ...

1994-01-01

493

Identification of three related human GRO genes encoding cytokine functions

Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.

1994-05-15

494

Human tissue factor pathway inhibitor (TFPI) gene: Complete genomic structure and localization on the genetic map of chromosome 2q

The product of the human GRO gene is a cytokine with inflammatory and growth-regulatory properties; GRO is also called MGSA for melanoma growth-stimulatory activity. The authors have identified two additional genes, GRO#beta# and GRO#gamma#, that share 90% and 86% identity at the deduced amino acid level with the original GRO#alpha# isolate. One amino acid substitution of proline in GRO#alpha# by leucine in GRO#beta# and GRO#gamma# leads to a large predicted change in protein conformation. Significant differences also exist in the 3' untranslated region, including different numbers of ATTTA repeats associated with mRNA instability. A 122-base-pair region in the 3' region is conserved among the three GRO genes, and a part of it is also conserved in the Chinese hamster genome, suggesting a role in regulation. DNA hybridization with oligonucleotide probes and partial sequence analysis of the genomic clones confirm that the three forms are derived ...

495

Genome lability in radiation-induced transformants of C3H 10T1/2 mouse fibroblasts

Tissue factor pathway inhibitor (TFPI), a protease inhibitor that circulates in association with plasma lipoproteins (VLDL, LDL and HDL), helps to regulate the extrinsic blood coagulation cascade. The authors have cloned a 125-kb genomic region containing the entire human TFPI gene on six overlapping cosmids and prepared a restriction map of this contig to clarify gene structure. More than half (45 kb) of the 85-kb gene is occupied with 5[prime] noncoding elements: coding begins at exon 3. A HindIII RFLP identified with one cosmid was genotyped in the CEPH panel of 559 reference families. Linkage analysis using markers on human chromosome 2 located the TFPI gene on 2q, 36 cM proximal to D2S43(pYNZ15) and 13 cM distal to the crystalline [gamma]-polypeptide locus CRYGP1(p5G1). 31 refs., 3 figs., 3 tabs.

1993-08-01

496

Construction of a human MluI YAC library

We have been investigating radiation-induced neoplastic transformants of C3H 10T1/2 mouse fibroblasts for evidence of heritable changes. C3H 10T1/2 cells were treated with 8 Gy X rays. After approximately 8 weeks of culture, type II/III foci were isolated from the monolayer using cloning rings. Cell lines developed from these foci, and clones established from these cell lines, were examined for DNA content. The isolated focus-derived populations and derived clones often display aneuploidy and/or polyploidization. In one instance a clone (derived from a single cell) displayed multiple polyploidies. During passage the ploidy of many of the anomalous populations gradually reverted to the ploidy of the non-neoplastically transformed state. The morphological features associated with the neoplastic transformation event were nevertheless retained. The results demonstrate that exposure to radiation can induce, in association with morphological neoplastic transformation, a heritable, ...

1994-04-01

497

A multi-marker model for detecting chromosomal segments displaying QTL activity

The authors describe a cloning strategy for the construction of a human genomic library in yeast artificial chromosomes (YACs) based on complete digestion of high-molecular-weight DNA with the infrequently cutting restriction enzyme MluI. Cloning of MluI fragments in the vector pYAC-RC and one subsequent size fractionation by preparative pulsed-field gel electrophoresis yielded a library with average insert sizes of 600 kb. Ninety-seven percent of the colonies were recombinant. An additional size fractionation of MluI fragments prior to ligation had no significant influence on the size of the YACs. The library currently consists of 5000 clones, which is the equivalent of one human genome. Nineteen percent of the YACs were larger than 1.2 Mb. Since smaller MluI fragments are lost during sizing, they also performed cloning without size fractionation. Only 20% of the colonies were recombinant, probably due to unligated vector fragments that were ...

1994-05-01

498

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

A statistical method is presented for detecting quantitative trait loci (QTLs), based on the linear model. Unlike methods able to detect a few well separated QTLs and to estimate their effects and positions, this method considers the genome as a whole and enables the detection of chromosomal segments involved in the differences between two homozygous lines, and their backcross, doubled haploid, or F[sub 2] progenies, for a quantitative trait. Genetic markers must be codominant, but missing markers are accepted, provided they are missing independently from the experiment. Asymptotic properties, which are of practical use, are developed. This method does not rely on strong genetic hypotheses, and thus does not permit any precise genetic analysis of the trait under study, but it does assess which regions of the genome are involved, whatever the complexity of the genetic determinism (number, effects and interactions among QTLs). Simultaneous use of ...

1993-08-01

499