e4 cloning sites: Topics by WorldWideScience.org

Branch morphology in young poplar clones on floodplain sites in ...

DNA from 48 hr germinated rice embryos was cut with restriction endonuclease Bam H1 and cloned to the Bam H1 site on plasmid pBR 322. The clones containing recombinant DNA were selected by their sensitivity to tetracycline and resistance to ampicillin. Using /sup 32/P-labelled rice embryos tRNA as a probe two clones were identified to contain tRNA genes by colony hybridization.

1981-10-20

2

Isolation and characterization of cDNA clones for human skeletal muscle alpha actin.

Sep 28, 2011 ... Description: Four Populus clones were grown in central Missouri for 2 years at 1 x 1 m spacing to study total biomass production on floodplain ...

3

Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

Two cDNA libraries corresponding to polyA+ RNA from human adult skeletal muscle have been constructed by cloning in the PstI site of pBR322. Skeletal alpha actin cDNA clones have been isolated and characterized....Full Text Available

1983-06-11

4

Growth dynamics and biomass production in short-rotation poplar plantations: 6-year results for three clones at four spacings

The authors have cloned a full-length putative rat pancreatic lysophospholipase cDNA by an improved mRNA isolation method and cDNA cloning strategy using (/sup 32/P)-labelled nucleotides. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long and relatively rare cDNA clone containing the entire sequence from the cap site at the 5' end to the poly(A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid composition, and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase.

1987-03-24

5

Characterization of mal recombination plasmids cloned in Streptococcus pneumoniae

Growth dynamics and biomass production in short-rotation poplar plantations were evaluated at Hanyuan Forestry Farm, Baoying County, P.R.C. Experimental treatments applied in a split-plot design included: four planting densities (1111, 833, 625 and 500 stems ha{sup -1}); three poplar clones (NL-80351, I-69 and I-72) and three rotation lengths. Survival of all three clones was excellent at the experimental site under the imposed management regime. Differences in DBH, height, LAI and above-ground biomass production, however, were manifested among the treatments. Overall the clonal ranking was in the order NL-80351 {>=}I-69 > I-72 by age 6. LAI values ranged from 0.6 to 4.4 m{sup 2} m{sup -2} and differences among four planting densities and three clones were significant throughout the first six years. Above-ground biomass productivity was affected considerably by planting density, ...

1999-07-01

6

[Cloning of the gene for thermostable Thermus aquaticus YT1 DNA polymerase and its expression in Escherichia coli].

The malM locus of Streptococcus pneumoniae was cloned into one of the two PstI sites of the multicopy S. pneumoniae plasmid pMV158. To eliminate chromosomal transformants in the simultaneous selection for tetracycline resistance (coded by pMV158) and maltose utilization, the host cells contained a chromosomal deletion of the mal gene cluster. Two clones were isolated; one with a 3.3 kb insert (pLS70) which behaved like wild type with respect to maltose utilization, and another with a 2.9 kb insert (pLS69) which behaved as though it contained a down promoter mutation. Preliminary mapping of these clones by restriction analysis placed the 0.4kb deletion on a HindIII fragment in the interior of the chromosomal insert. The recombinant plasmids were able to transform over 50% of a recipient population to Mal/sup +/. Enzyme measurements of the clones indicated an overproduction of ...

1981-01-01

7

Human thrombomodulin gene is intron depleted: nucleic acid sequences of the cDNA and gene predict protein structure and suggest sites of regulatory control.

Using the phasmid vector pSL5, the genomic DNA fragment of T. aquaticus YT1 which contained the thermostable DNA polymerase (Taq-polymerase) gene was cloned. The BglII fragment of this genome locus was subcloned in the BamHI site of the pUC19 plasmid. To optimize the Taq-polymerase gene expression in E. coli cells, the gene was cloned in the correct reading frame regarding the initiation ATG codon of the pPR-TGATG-1 expression vector. The gene expression in this vector was controlled by the phage lambda PR promoter and the temperature-sensitive phage lambda repressor. We used PCR to amplify the short 5'-end fragment of the Taq-polymerase gene coding for the part into which an artificial SacI site was introduced. This site has been used for cloning the PCR product into the pPR-TGATG-1 vector, and the missing gene part was cloned into the KpnI ...

8

Sequences of versatile, broad-host-range vectors of the RK2 family.

We have isolated a human thrombomodulin cDNA, and a human genomic clone containing the putative promoter domain, as well as the translated and untranslated regions of the endothelial cell receptor....Full Text Available

1987-09-01

9

Expression of bacterial luciferase in eukaryotic cells

Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available.

2003-07-01

10

On the parameterization of the roughness length for the air-sea interface in free convection for the coastal site Tarapur, India

Expression of Bacterial luciferase enzyme (lux) in mammalian cells would be a powerful bioreporter protein system for in vivo imaging because eukaryotic luciferases need expensive substrates. However, only a few efforts have been made to express bacterial luciferase enzyme in mammalian cells. As the result of this, we attempted to construct bicistronic vector including two bacterial luciferase genes (LuxA and LuxB) for assessing the potential to be visualized in vitro or in vivo by optical imaging system after transfection to mammalian cells. We designed and synthesized luxA and luxB genes from Photorhabdus Luminescens. To co-express both luxA and luxB genes from a single promoter, we cloned as a bicistronic transcript fused with an internal ribosomal entry site (IRES). This bicistronic transcript was transfected by Superfect to HEK 293T cell line. We also transfected lux A and lux B vector to HEK 293T cells separately. To evaluate gene ...

2005-11-18

11

The roughness length at air-sea interface during free convection (Z0fc) is mainly related to the convective velocity (w) rather than the friction velocity (u). The parameterization of Z0fc with (w)2/g as proposed by Abdella and D'Alessio (2003) is evaluated. It is shown that the field measurements at MM Lab, Tarapur Maharashtra Site (TMS) coastal site using Metek GmbH, Ultra sonic anemometers are consistent with the proposed formula. In order to avoid self-correlation by using u, a new parameterization of w with ?u and ?v and gustiness parameter as given by Fairall et al. (1996) is used. The mean values of w and Z0fc estimated using new parameterization were observed to be 0.97 m/s and 2.3E-4 m respectively for the year 2009 at TMS. (author)

2010-05-13

12

Human Cloning

Vegetative propagation of hybrid aspen by bud grafting

... is known as "reproductive cloning." The Link to Stem Cell Research Stem cell research and research cloning are closely linked. Scientists in ... to arrive at a position on cloning and stem cell research. Many nations, including the UK, China, and South ...

13

Cloning of Drosophila transcription factor Adf-1 reveals homology to Myb oncoproteins.

Results are presented of trials in W. Germany involving Populus tremula X P. tremuloides. In a nursery in late August - early September 1971, some 1300 buds from 38 selected 3-yr-old hybrid aspen seedlings were grafted into T-slits at nodes 10-20 cm above root' collars of 1 and a half yr-old hybrid aspen stock (products of controlled crossings, and in some cases of similar parentage to the transplanted buds). In spring 1972, each stock (from which its own buds were removed) was cut back to a 'peg' 15-20 cm above the level of the graft; the 'peg' was later used to support the new shoot developed from the transplanted bud. By Sept. 1972, 33% of grafts were successful, the success rate being significantly different (3-67%) within clones; average height of scion shoots ranged from 79 to 375 cm (no significant difference between clones). In spring 1973, some 190 grafts from 35 clones ...

1982-01-01

14

cDNA cloning, sequence analysis and organ distribution of horse preproendothelin-2.

The Drosophila sequence-specific DNA binding protein, Adf-1, is capable of activating transcription of the alcohol dehydrogenase gene, Adh, and is implicated in the transcriptional control of other developmentally regulated genes. We have cloned the cDNA encoding Adf-1 by generating specific DNA probes deduced from partial amino acid sequence of the protein. Several cDNA clones encoding an extended open reading frame were isolated from a phage lambda library. The complete amino acid sequence of Adf-1 deduced from the longest cDNA reveals structural similarities to the putative helix-turn-helix DNA binding motif of Myb and Myb-related proteins. DNA sequence analysis of genomic clones and Northern blot analysis of mRNA suggest that Adf-1 is a single-copy gene encoding a 1.9-kb transcript. Purified recombinant Adf-1 expressed in Escherichia coli binds specifically to Adf-1 recognition sites and activates ...

1992-01-15

15

Molecular resemblance of an AIDS-associated lymphoma and endemic Burkitt lymphomas: Implications for their pathogenesis

We cloned and characterized horse preproendothelin-2 (PPET-2) cDNA from intestinal tissue. The cDNA encoded 178 amino acids of the PPET-2 polypeptide, in which a 21-amino-acid mature endothelin-2 peptide and a 16-amino acid endothelin-2-like peptide were found. For the open reading frame the correspondence of horse PPET-2 cDNA with those of the ferret, human, dog, mouse and rat was 85.1%, 84.9%, 82.1%, 77.8% and 77.2%, respectively. Analysis of the organ distribution of PPET-2 mRNA by reverse transcription-polymerase chain reaction demonstrated that the kidney, stomach and small intestine are major sites of expression of the PPET-2 gene. Surprisingly, the mRNA is not detected in the large intestine, where high expression is demonstrated in the mouse and rat. This difference may result from the underlying functional differences of the large intestine between a herbivore (horse) and an omnivore (mouse and rat). PMID:15838341

2004-11-01

16

Molecular cloning, genomic organization, and chromosomal localization of the human pancreatitis-associated protein (PAP) gene

Non-Hodgkin lymphoma is a common feature of AIDS. Approximately 30-40% of these tumors exhibit clinical features suggestive of endemic Burkitt lymphoma: they are aggressive malignancies that occur in association with Epstein-Barr virus infection, they arise in the setting of immunosuppression, and they carry t(8;14) translocations without detectable rearrangement of the MYC oncogene. To understand the molecular basis of these parallels, the authors analyzed a case of Epstein-Barr-positive AIDS-associated undifferentiated lymphoma. Southern blots show that the tumor exhibits immunoglobulin joining segment rearrangement but no rearrangement of the MYC oncogene. Cloning of the rearranged joining segment allowed the isolation of recombinant clones encompassing the translocation breakpoint, and sequencing of the translocation junction disclosed that the breakpoint is situated 7 base pairs from the chromosome 14 site involved in ...

17

Should we clone human beings? Cloning as a source of tissue for transplantation.

Pancreatitis-associated protein (PAP) is a secretory pancreatic protein present in small amounts in normal pancreas and overexpressed during the acute phase of pancreatitis. In this paper, the authors describe the cloning, characterization, and chromosomal mapping of the human PAP gene. The gene spans 2748 bp and contains six exons interrupted by five introns. The gene has a typical promoter containing the sequences TATAAA and CCAAT 28 and 52 bp upstream of the cap site, respectively. They found striking similarities in genomic organization as well as in the promoter sequences between the human and rat PAP genes. The human PAP gene was mapped to chromosome 2p12 using rodent-human hybrid cells and in situ chromosomal hybridization. This localization coincides with that of the reg/lithostathine gene, which encodes a pancreatic secretory protein structurally related to PAP, suggesting that both genes derived from the same ancestral gene by ...

1994-01-01

18

Pathogenicity of molecularly cloned bovine leukemia virus.

The most publicly justifiable application of human cloning, if there is one at all, is to provide self-compatible cells or tissues for medical use, especially transplantation. Some have argued that...Full Text Available

1999-04-01

19

An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites

To delineate the mechanisms of bovine leukemia virus (BLV) pathogenesis, four full-length BLV clones, 1, 8, 9, and 13, derived from the transformed cell line FLK-BLV and a clone construct, pBLV913,...Full Text Available

1993-12-01

20

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit gene: Cloning, mapping, structure, and targeting in mouse

BackgroundObtaining single parasite clones is required for many techniques in malaria research. Cloning by limiting dilution using microscopy-based assessment for parasite growth...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

21

Construction of a genome-wide human BAC-Unigene resource. Final progress report, 1989--1996

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit is a member of a family of ligand-gated ion channels, and is the only subunit know to bind {alpha}-bungarotoxin in mammalian brain. {alpha}-Bungarotoxin binding sites are known to be more abundant in the hippocampus of mouse strains that are particularly sensitive to nicotine-induced seizures. The {alpha}7 receptor is highly permeable to calcium, which could suggest a role in synaptic plasticity in the nervous system. Auditory gating deficiency, an abnormal response to a second auditory stimulus, is characteristic of schizophrenia. Mouse strains that exhibit a similar gating deficit have reduced hippocampal expression of the {alpha}7 subunit. We have cloned and sequenced the full length cDNA for the mouse {alpha}7 gene (Acra-7) and characterized its gene structure. The murine {alpha}7 shares amino acid identity of 99% and 93% with the rat and human {alpha}7 subunits, respectively. ...

1994-09-01

22

Therapeutic cloning in the mouse

Currently, over 30,000 mapped STSs and 27,000 mapped Unigenes (non-redundant, unigene sets of cDNA representing EST clusters) are available for human alone. A total of 44,000 Unigene cDNA clones have been supplied by Research Genetics. Unigenes, or cDNAs are excellent resource for map building for two reasons. Firstly, they exist in two alternative forms -- as both sequence information for PCR primer pairs, and cDNA clones -- thus making library screening by colony hybridization as well as pooled library PCR possible. The authors have developed an efficient and robust procedure to screen genomic libraries with large number of DNA probes. Secondly, the linkage and order of expressed sequences, or genes are highly conserved among human, mouse and other mammalian species. Therefore, mapping with cDNA markers rather than random anonymous STSs will greatly facilitate comparative, evolutionary studies as well as physical map building. They have ...

1996-12-31

23

Molecular cloning and analysis of lymphokines. Volume 13

Nuclear transfer technology can be applied to produce autologous differentiated cells for therapeutic purposes, a concept termed therapeutic cloning. Countless articles have been published on the ethics...Full Text Available

2003-09-30

24

Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male ...

These proceedings collect papers on the subject of lymphokines. Topics include: DNA-cloning of mouse and human lymphokine genes, inteferons, interleukins, gene expression, tumor necrosis factors, and recombinant DNA.

1987-01-01

25

Generation and Characterization of Monoclonal Antibodies to Human Keratinocyte Growth Factor Receptor

... 041889 Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male Reproducti...

26

British Library Electronic Table of Contents (United Kingdom)

Keratinocyte growth factor receptor (KGFR) and fibroblast growth factor receptor (FGFR) 2c share identical amino acid sequences, except for a 46-amino acid domain in the extracellular region. Monoclonal antibodies (MAbs) specific to KGFR have not been reported nor are commercially available. In this study, we generated murine MAbs specific to KGFR in non-obese diabetic (NOD) mice using a modified Repeated Immunizations at Multiple Sites (RIMMS) technology. Stable cell lines expressing the full-length human KGFR or FGFR2c were produced to facilitate the identification of KGFR-specific MAbs. Following the initial screening of hybridoma clones with a fluorescence-based, confocal cell detection method and ELISA, KGFR-specific MAbs were selected and confirmed by flow cytometry and Western blot ...

2006-01-01

27

Regulation of cloned, Ca2+-activated K+ channels by cell volume changes.

DEFF Research Database (Denmark)

Udgivelsesdato: 2002-May

2002-01-01

28

Molecular Cloning and Characterization of the Yew Gene Encoding Squalene Synthase from Taxus cuspidata

Expression and Characterization of a New Esterase Cloned Directly from Agrobacterium tumefaciens Genome

Full Text Available

2007-09-01

29

Responses of hybrid poplar clones and red maple seedlings to ambient O_3 under differing light within a mixed hardwood forest

Full Text Available

2006-01-01

30

Nuclear reprogramming in mammalian somatic cell nuclear cloning

The responses of ramets of hybrid poplar (Populus spp.) (HP) clones NE388 and NE359, and seedlings of red maple (Acer rubrum, L.) to ambient ozone (O_3) were studied during May-September of 2000 and 2001 under natural forest conditions and differing natural sunlight exposures (sun, partial shade and full shade). Ambient O_3 concentrations at the study site reached hourly peaks of 109 and 98 ppb in 2000 and 2001, respectively. Monthly 12-h average O_3 concentrations ranged from 32.3 to 52.9 ppb. Weekly 12-h average photosynthetically active radiation (PAR) within the sun, partial shade and full shade plots ranged from 200 to 750, 50 to 180, and 25 to 75 #mu#mol m"-"2 s"-"1, respectively. Ambient O_3 exposure induced visible foliar symptoms on HP NE388 and NE359 in both growing seasons, with more severe injury observed on NE388 than on NE359. Slight foliar symptoms were observed on red maple seedlings during the 2001growing season. Percentage of ...

2004-07-01

31

Molecular cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.

Nuclear cloning is still a developing technique used to create genetically identical animals by somatic cell nuclear transfer into unfertilized eggs. Despite an intensive effort in a number...Full Text Available

2004-01-01

32

Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera

This article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of Actinobacillus pleuropneumoniae. The hemolysin was a thermolabile protein with an apparent...Full Text Available

1989-11-01

33

Induced pluripotent stem cells, new tools for drug discovery and new hope for stem cell therapies

Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing...Full Text Available

2011-01-01

34

Human cytoplasmic actin proteins are encoded by a multigene family

Somatic cell nuclear transfer or therapeutic cloning has provided great hope for stem cell-based therapies. However therapeutic cloning has been experiencing both ethical and technical difficulties....Full Text Available

2009-01-01

35

Genomic cloning and characterization of a ricin gene from Ricinus communis.

The authors characterized nine human actin genes that they isolated from a library of cloned human DNA. Measurements of the thermal stability of hybrids formed between each cloned actin gene and ..cap alpha..-, ..beta..-, and ..gamma..-actin mRNA demonstrated that only one of the clones is most homologous to sarcomeric actin mRNA, whereas the remaining eight clones are most homologous to cytoplasmic actin mRNA. By the following criteria they show that these nine clones represent nine different actin gene loci rather than different alleles or different parts of a single gene: (i) the restriction enzyme maps of the coding regions are dissimilar; (ii) each clone contains sufficient coding region to encode all or most of an entire actin gene; and (iii) each clone contains sequences homologous to both the 5' and 3' ends of the ...

1982-06-01

36

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor...Full Text Available

1985-11-25

37

Cloning and expression of recombinant, functional ricin B chain.

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

38

Short-term regulation of muscarinic acetylcholine receptors: An assessment utilizing mouse brain and mouse neuroblastoma cells

The cDNA encoding the B chain of the plant toxin ricin has been cloned and expressed in monkey kidney COS-M6 cells. The recombinant B chain was detected by labeling the transfected cells with [35S]methionine...Full Text Available

1987-08-01

39

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

The effects of muscarinic agonists and diisopropylfluorophosphate (DFP) on muscarinic receptor density and muscarinic receptor-mediated responses was assessed in mouse brain and mouse neuroblastoma cells (clone N1E-115). Utilizing the antagonist ({sup 3}H)quinuclidinyl benzilate (({sup 3}H)QNB), there was no difference in the maximal binding capacity (B{sub max}) or equilibrium dissociation constant (K{sub d}) between untreated and 24 hour DFP-treated mice. However, one administration of DFP produced a 24% and 33% decrease in B{sub max} measured by ({sup 3}H)N-methylscopolamine (({sup 3}H)NMS) after 18 and 24 hours which was rapidly reversible within 36 hours after DFP treatment. The loss of ({sup 3}H)NMS binding sites following acute DFP treatment was not accompanied by a change in a particular muscarinic receptor binding conformation. Furthermore, the magnitude of muscarinic receptor-mediated phosphoinositide hydrolysis was unchanged ...

1988-01-01

40

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to ...

1988-10-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

41

RFLP for a DNA clone which maps to 19q13. 2-19qter (D19S63)

We have isolated a genomic clone containing 'Lupinus luteus' 5S ribosomal RNA genes by screening with 5S rDNA probe clones that were hybridized previously with the initiator methionine tRNA preparation (contaminated) with traces of rRNA or its degradation products). The clone isolated contains ten repeat units of 342 bp with 119 bp fragment showing 100% homology to the 5S rRNA from yellow lupine. Sequence analysis indicates only point heterogeneities among the flanking regions of the genes. (author). 6 refs, 3 figs.

1993-01-01

42

Chromosomal localization and cDNA sequence of human BTEB, a GC box binding protein

pD10 is a 0.85 kb fragment with BamHI ends cloned in pSP64. It was sub-cloned from {lambda}D10 which was isolated from a genomic library constructed in {lambda}EMBL3 from a rodent human somatic cell hybrid. The probe was localized to 19q13.2-19qter on a panel of rodent human somatic cell hybrids. Co-dominant segregation was shown in 32 families of 280 individuals.

1990-02-25

43

Seasonal and clonal variations in technological and thermal properties of raw Hevea natural rubber

Human BTEB cDNA clones have been isolated, sequenced, and the corresponding gene has been assigned to human chromosome 9, region q13, by fluorescent in situ hybridization and DNA blot analysis using DNAs from hybrid cell clones containing a single human chromosome. The cDNA clone encodes a polypeptide of 244 amino acids whose sequence shows a high sequence similarity with the rat BTEB (98% amino acid identity).

1993-09-01

44

British Library Electronic Table of Contents (United Kingdom)

Abstract This study was undertaken over a 10-month period under environmental conditions within the state of Mato Grosso, Brazil, to evaluate the causes of variation in the technological and thermal properties of raw natural rubber (NR) from different clones of Hevea brasiliensis (GT 1, PR 255, FX 3864, and RRIM 600). These clones were chosen to represent good clones available in Brazil. The technological properties of raw NR were evaluated in terms of their dry rubber content (DRC), Wallace plasticity, plasticity retention index (PRI), and Mooney viscosity. The thermal performance was evaluated with the thermogravimetry (TG)/differential thermogravimetry (DTG) technique. There were significant variations (p < 0.01 and p < 0.05) between clones and tappings for all technological properties,...

2011-01-01

45

Cloning of the neurodegeneration gene drop-dead and characterization of additional phenotypes of its mutation

Cloning of the cDNA and gene for a human D sub 2 dopamine receptor

Mutations in the Drosophila gene drop-dead (drd) result in early adult lethality and neurodegeneration, but the molecular identity of the drd...Full Text Available

2008-01-01

46

A DNA recombinant database management system.

A clone encoding a human D{sub 2} dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D{sub 2} receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

1989-12-01

47

Closure of Off-Site Telnet

A set of computer programs is described which constitutes a clone database management system. Maintenance of the database and the stocks of material is designed to be under the control of one person...Full Text Available

1983-07-11

48

Aerial view of CERN with the LHC sites.

Closure of Off-Site Telnet

2002-01-01

49

The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.

Aerial view of CERN with the LHC sites.

1998-01-01

50

The Differentially Expressed Genes by Radiotherapy in the Patients with Uterine Cervix Cancer

We have isolated and sequenced cDNA clones encoding the human U1-70K snRNP protein, and have mapped this locus (U1AP1) to human chromosome 19. The gene produces two size classes of RNA, a major 1.7-kb...Full Text Available

1987-12-23

51

Synthesis and cloning of the genes of antisense peptides of human calcitonin and miniproinsulin

Purpose : To detect differentially expressed genes in the patients with uterine cervical cancer during the radiation therapy. Materials and Methods : In patients with biopsy proven uterine cervical cancer, we took a tumor tissue just before radiation therapy and at 40 minutes after external irradiation of 1.8 Gy. Total RNAs isolated from non-irradiated and irradiated tumor tissue samples were analyzed using the differential-display reverse transcription-polymerase chain reaction (DDRT-PCR). Complementary DNA (cDNA) fragments corresponding to differentially expressed messenger RNAs(mRNAs) were eluted, and cloned. The differential expression of the corresponding mRNAs was confirmed by reverse northern blot. Differentially expressed cDNA bands were sequenced. Nucleotide sequence data were analyzed in the Gene Bank and EMBL databases via the BLAST network server to identify homologies to known genes or cDNA fragments. Expression pattern of down-regulated ...

2001-12-15

52

Plasmids for the cloning and expression of full-length double-stranded cDNAs under control of the SV40 early or late gene promoter.

With the aim of an experimental check on the validity of the theory of molecular recognition, the authors have carried out the chemical-enzymatic synthesis and cloning of the gene of human calcitonin and also of the genes of antisense polypeptides to human calcitonin and miniproinsulin. It has been shown that recombinant plasmids obtained on the basis of these synthetic genes are capable of ensuring the biosynthesis of the given polypeptides in E. coli cells as hybrid proteins with the IgG-binding domain of staphylococcal protein A.

1994-07-20

53

Ordered Cloned DNA Map of the Genome of Vibrio cholerae 569B and Localization of Genetic Markers

Okayama and Berg (1) have recently described a technique for the high efficiency cloning of full-length dscDNAs. We have constructed eukaryotic expression vectors compatible both with this technique...Full Text Available

1983-10-25

54

Molecular Cloning and Evidence for Osmoregulation of the ?¹-Pyrroline-5-Carboxylate Reductase (proC) Gene in Pea (Pisum sativum L.) ¹²

By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol. 176:1105–1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic...Full Text Available

1998-02-01

55

Elimination of L-A double-stranded RNA virus of Saccharomyces cerevisiae by expression of gag and gag-pol from an L-A cDNA clone.

Several cDNA clones encoding Δ¹-pyrroline-5-carboxylate reductase (P5CR, l-proline:NAD[P]⁺ 5-oxidoreductase, EC 1.5.1.2), which catalyzes the terminal step in...Full Text Available

1992-11-01

56

Decay-accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno-focal cloning method.

We report that expression of a nearly full-length cDNA clone of the L-A double-stranded RNA virus causes virus loss in a wild-type strain of Saccharomyces cerevisiae. We show that in this system exclusion...Full Text Available

1993-05-01

57

Cloning and characterization of a 77-kDa oestrogen receptor isolated from a human breast cancer cell line.

Using an anti-receptor mAb that blocks the attachment of echovirus 7 and related viruses (echoviruses 13, 21, 29 and 33), we have isolated a complementary DNA clone that encodes the human decay-accelerating...Full Text Available

1994-11-01

58

A detailed comparison of the 5'-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA.

We have cloned and characterized a 77-kDa oestrogen receptor (ER) from an oestrogen-independent subclone of the MCF-7 human breast cancer cell line. This receptor contains an in-frame, tandem duplication...Full Text Available

1997-01-01

59

Tissue culture and micropropagation for forest biomass production

We have examined homologous fragments of DNA cloned from two different tissues for changes in the dNA sequence which might be related to tissue specific gene expression. The 5' end of the chicken ovalbumin...Full Text Available

1980-10-10

60

Structure, Function, and Evolution of Rice Centromeres

An increase in forest production will be necessary in the future when wood becomes a major renewable source of energy and chemicals along with its traditional role of fibre source. This increase could eventually by achieved be proper selection and breeding of trees. Clonal forestry by vegetative propagation of cuttings is becoming a viable alternative to a seedling-based forestry with many advantages, and cutting could be used to quickly propagate large numbers of clones of control-pollinated seedlings. Most forest trees are propagated sexually and seed orchards were started in the US and Canada in the last 40-50 years for breeding purposes. Forests could ultimately be established with improved seedlings instead of from seed with unknown genetic potential, or by natural regeneration. Micropropagation is the term used to refer to the propagation of plants raised by tissue culture methods rather than from seeds or cuttings. Many clonal plantlets could be regenerated ...

1984-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

61

Genetic and physical mapping of the Chediak-Higashi syndrome on chromosome 1q42-43

The centromere is the most characteristic landmark of eukaryotic chromosomes. Centromeres function as the site for kinetochore assembly and spindle attachment, allowing for the faithful pairing and segregation of sister chromatids during cell division. Characterization of centromeric DNA is not only essential to understand the structure and organization of plant genomes, but it is also a critical step in the development of plant artificial chromosomes. The centromeres of most model eukaryotic species, consist predominantly of long arrays of satellite DNA. Determining the precise DNA boundary of a centromere has proven to be a difficult task in multicellular eukaryotes. We have successfully cloned and sequenced the centromere of rice chromosome 8 (Cen8), representing the first fully sequenced centromere from any multicellular eukaryotes. The functional core of Cen8 spans ~800 kb of DNA, which was determined by chromatin immunoprecipitation ...

2010-02-04

62

Assignment of the human aggrecan gene (AGC1) to 15q26 using fluorescence in situ hybridization analysis

The Chediak-Higashi syndrome (CHS) is a severe autosomal recessive condition, features of which are partial oculocutaneous albinism, increased susceptibility to infections, deficient natural killer cell activity, and the presence of large intracytoplasmic granulations in various cell types. Similar genetic disorders have been described in other species, including the beige mouse. On the basis of the hypothesis that the murine chromosome 13 region containing the beige locus was homologous to human chromosome 1, we have mapped the CHS locus to a 5-cM interval in chromosome segment 1q42.1-q42.2. The highest LOD score was obtained with the marker D1S235 (Z{sub max} = 5.38; {theta} = 0). Haplotype analysis enabled us to establish D1S2680 and D1S163, respectively, as the telomeric and the centromeric flanking markers. Multipoint linkage analysis confirms the localization of the CHS locus in this interval. Three YAC clones were found to cover the entire region in a contig ...

1996-09-01

63

Alterations in heart looping induced by overexpression of the tight junction protein Claudin-1 are dependent on its C-terminal cytoplasmic tail.

The large aggregating proteoglycan aggrecan is a major structural component of the extracellular matrix of articular cartilage. Recent cDNA cloning of the human aggrecan gene (AGC1) reveals a core protein of at least 2316 amino acids characterized by several distinct structural domains. Two globular domains, termed G1 and G2, are present at the amino terminus of the molecule and a third, termed G3, is present at the carboxy terminus. The G1 domain is homologous in structure to the cartilage link protein and accounts for the aggregating potential of aggrecan through its ability to interact with hyaluronic acid. The aggrecan gene is known to consist of 15 exons, with each exon encoding a distinct functional region of the mature protein. However, while the link protein gene is known to reside on chromosome 5 in the human, the location of the aggrecan gene is currently undetermined in any species. The probe (pAGG2) for the aggrecan gene was mapped on chromosome band ...

1993-05-01

64

Membrane alteration is necessary but not sufficient for effective glutamate secretion in Corynebacterium glutamicum.

In vertebrates, the positioning of the internal organs relative to the midline is asymmetric and evolutionarily conserved. A number of molecules have been shown to play critical roles in left-right patterning. Using representational difference analysis to identify genes that are differentially expressed on the left and right sides of the chick embryo, we cloned chick Claudin-1, an integral component of epithelial tight junctions. Here, we demonstrate that retroviral overexpression of Claudin-1, but not Claudin-3, on the right side of the chick embryo between HH stages 4 and 7 randomizes the direction of heart looping. This effect was not observed when Claudin-1 was overexpressed on the left side of the embryo. A small, but reproducible, induction of Nodal expression in the perinodal region on the right side of the embryo was noted in embryos that were injected with Claudin-1 retroviral particles on their right sides. However, no changes in Lefty,Pitx2 or cSnR ...

2006-02-24

65

Effects of Environmental Factors on Microbial Populations in Brackish Waters off the Southern Coast of Finland

We showed recently that secretion of glutamate in biotin-limited cells of Corynebacterium glutamicum is mediated by carrier systems in the plasma membrane (C. Hoischen and R. Kr�e4;mer, Arch. Microbiol....Full Text Available

1990-06-01

66

Amyloid Load in Nondemented Brains Correlates with APOE e4

The roles played by environmental factors in seasonal changes in microbial populations were investigated in the Tv�e4;rminne area, off the southern coast of Finland. Surface-layer samples were...Full Text Available

1980-07-01

67

Activity-Local Symbolic State Graph Generation for High-Level ...

179 cognitively healthy adults enrolled in the Sun Health Brain Donation program between 7/91 and 12/07 were at least 60 years old and nondemented at the time of death (21 had developed mild...Full Text Available

2010-04-12

68

var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2

... ie on all paths from the root to a terminal node we ... (A) A stochastic Petri net ... Tandem Queueing Network [HMKS99] 63 26 19 173 2.297e4 7.148e ...

2005-09-01

69

[Molecular cloning and expression of an isotoxin gene, alpha-bungarotoxin, from Bungarus multicinctus].

BackgroundThe pathogenicity of Plasmodium falciparum is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell...Full Text Available

70

Yeast artificial chromosome libraries containing large inserts from mouse and human DNA

Abstract: Snake venom contains a number of small proteins,enzymes and other components,which displays a broad spectrum of biological activities. With the ability of specifically binding on acetylcholine acceptor, alpha-bungarotoxins are not only useful molecular probes in investigating the mechanism of neural signal transmission, but also potential pharmic preparations for neural disease treatment. In current research,cDNAs of Bungarus multicinutus venom gland were synthesized using SMART cDNA amplification kit and then, alpha-bungarotoxin genes were cloned and sequenced. Total of 20 clones were sequenced representing 14 isotoxin mRNAs of alpha-bungarotoxins. Among those clones, a novel isotoxin gene was subcloned into two expression plasmids, alpha-BgTX/pQE30a and alpha-BgTX/pGEX-4T-1, and transformed into E. coli. After inducing with IPTG, fused protein of GST-alpha-BgTX was successfully expressed at level of 30% gross ...

2005-07-01

71

Vitamin E Is Essential for Seed Longevity and for Preventing Lipid Peroxidation during Germination

Yeast artificial chromosome (YAC) libraries have been difficult to construct with average insert sizes >400 kilobase pairs when DNA is size-fractionated in low-melting-point agarose. By using yeast chromosomes in mock cloning experiments, the authors found that polyamines should be present whenever agarose containing high molecular weight DNA is melted to protect DNA from degradation. By incorporating polyamines during the cloning procedure, they constructed YAC libraries from mouse and human DNA with average insert sizes of 700 and 620 kilobase pairs, respectively. Several genome equivalents of these YAC libraries were replicated onto the surface of many duplicate agar plates using a 40,000 multipin transfer device. High-density filter replicas were screened by hybridization, and 70 mouse YAC clones from 31 loci and 132 human YAC clones from 49 loci were isolated.

1991-05-15

72

Survival of Genetically Modified and Self-Cloned Strains of Commercial Baker's Yeast in Simulated Natural Environments: Environmental Risk Assessment

Tocopherols (vitamin E) are lipophilic antioxidants synthesized by all plants and are particularly abundant in seeds. Despite cloning of the complete suite of tocopherol biosynthetic enzymes and successful...Full Text Available

2004-06-01

73

Streptococcus pneumoniae Isolates with Reduced Susceptibility to Ciprofloxacin in Spain: Clonal Diversity and Appearance of Ciprofloxacin-Resistant Epidemic Clones

Although genetic engineering techniques for baker's yeast might improve the yeast's fermentation characteristics, the lack of scientific data on the survival of such strains in natural environments...Full Text Available

2005-11-01

74

Spread of a highly mucoid Streptococcus pyogenes emm3/ST15 clone

Analysis of the pulsed-field gel electrophoretic profiles of 82 pneumococcal isolates with reduced susceptibility to ciprofloxacin (RSC) and of 90 co-occurring susceptible isolates indicates a considerable...Full Text Available

2001-10-01

75

Spread of a Salmonella typhimurium Clone Resistant to Expanded-Spectrum Cephalosporins in Three European Countries

BackgroundHyaluronic acid capsule plays a key role in Streptococcus pyogenes virulence. Circulation of mucoid or highly encapsulated strains has been related to...Full Text Available

76

Specific genetic modifications of domestic animals by gene targeting and animal cloning

Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 to 1998 in Russia, Hungary, and Greece. Resistance...Full Text Available

1999-11-01

77

Rooting of hybrid clones of Populus tremula L. x P. tremuloides Michx. by stem cuttings derived from micropropagated plants

The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large...Full Text Available

78

Retinoic acid X receptor in the diploblast, Tripedalia cystophora

Propagation costs could be cut by replacing part of the micropropagation process with steps involving more traditional techniques. This study explored possibilities for improving existing vegetative propagation techniques for aspen using stem cuttings obtained from micropropagated plants. Vegetative propagation through stem cuttings was studied in 10 micropropagated hybrid aspen clones (Populus tremula L. x P. tremuloides Michx). Cuttings containing one axillary bud were harvested from the same donor plants twice during the growing season: the first harvest in May and the second harvest in July. Rooting percentage was correlated positively with root length, number of roots and height of cutting plant but negatively with length of rooting. The average rooting percentage was 53% in the first harvest and 27% in second harvest. Indole-3-butyric acid treatments (1.2 mM) significantly improved rooting in the second harvest, but not in the first harvest, suggesting ...

2001-07-01

79

Recovery of Pathogenic Measles Virus from Cloned cDNA

Nuclear hormone receptors comprise a characteristic family of transcription factors found in vertebrates, insects and nematodes. Here we show by cDNA and gene cloning that a Cnidarian, Tripedalia...Full Text Available

1998-11-10

80

Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5_Hc Gene by Allelic Replacement

Reverse genetics technology so far established for measles virus (MeV) is based on the Edmonston strain, which was isolated several decades ago, has been passaged in nonlymphoid cell lines, and is no...Full Text Available

2000-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

81

Probiotic Lactobacillus spp. Diminish Helicobacter hepaticus-Induced Inflammatory Bowel Disease in Interleukin-10-Deficient Mice

URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5...Full Text Available

1998-10-01

82

Osteopontin is elevated during neointima formation in rat arteries and is a novel component of human atherosclerotic plaques.

Clinical and experimental evidence has demonstrated the potential role of probiotics in the prevention or treatment of inflammatory bowel disease. Probiotic clones with direct immunomodulatory activity...Full Text Available

2005-02-01

83

Mosquitocidal activity of the CryIC delta-endotoxin from Bacillus thuringiensis subsp. aizawai.

In an earlier report, we used differential cloning to identify genes that might be critical in controlling arterial neointima formation (Giachelli, C., N. Bae, D. Lombardi, M. Majesky, and S. Schwartz....Full Text Available

1993-10-01

84

Molecular cloning and nucleotide sequence of the beta-lytic protease gene from Achromobacter lyticus.

The cloned 135-kDa CryIC delta-endotoxin from Bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against Spodoptera litoralis and Spodoptera frugiperda larvae and...Full Text Available

1996-02-01

85

Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohlii Briq

Two bacteriolytic enzymes secreted by Achromobacter lyticus M497-1 were purified and identified as being very similar (considering their amino acid composition and N-terminal sequence) to alpha- and...Full Text Available

1990-11-01

86

Methanogen Diversity Evidenced by Molecular Characterization of Methyl Coenzyme M Reductase A (mcrA) Genes in Hydrothermal Sediments of the Guaymas Basin

BackgroundIsopentenyl diphosphate (IPP), a common biosynthetic precursor to the labdane diterpene forskolin, has been biosynthesised via a non-mevalonate pathway. Geranylgeranyl...Full Text Available

87

Genome lability in radiation-induced transformants of C3H 10T1/2 mouse fibroblasts

The methanogenic community in hydrothermally active sediments of Guaymas Basin (Gulf of California, Mexico) was analyzed by PCR amplification, cloning, and sequencing of methyl coenzyme M reductase...Full Text Available

2005-08-01

88

Factors Influencing the Tissue Culture and the Agrobacterium tumefaciens-Mediated Transformation of Hybrid Aspen and Poplar Clones

We have been investigating radiation-induced neoplastic transformants of C3H 10T1/2 mouse fibroblasts for evidence of heritable changes. C3H 10T1/2 cells were treated with 8 Gy X rays. After approximately 8 weeks of culture, type II/III foci were isolated from the monolayer using cloning rings. Cell lines developed from these foci, and clones established from these cell lines, were examined for DNA content. The isolated focus-derived populations and derived clones often display aneuploidy and/or polyploidization. In one instance a clone (derived from a single cell) displayed multiple polyploidies. During passage the ploidy of many of the anomalous populations gradually reverted to the ploidy of the non-neoplastically transformed state. The morphological features associated with the neoplastic transformation event were nevertheless retained. The results demonstrate that exposure to radiation can induce, ...

1994-04-01

89

Expression and Characterization of the Mycobacterium tuberculosis Serine/Threonine Protein Kinase PknB

Tissue culture conditions and transformation have been established for both aspen and poplar. The use of previously described culture conditions resulted in shoot tip necrosis in the shoot cultures...Full Text Available

1990-07-01

90

Development of an extrachromosomal cloning vector system for use in Borrelia burgdorferi

PknB is a member of the newly discovered eukaryotic-like protein serine/threonine kinase (PSTK) family of proteins. The pknB gene was cloned and expressed in Escherichia coli....Full Text Available

1999-11-01

91

Construction of a human MluI YAC library

Molecular genetic analysis of Borrelia burgdorferi, the cause of Lyme disease, has been hampered by the absence of any means of efficient generation, identification, and complementation...Full Text Available

2000-04-25

92

Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV.

The authors describe a cloning strategy for the construction of a human genomic library in yeast artificial chromosomes (YACs) based on complete digestion of high-molecular-weight DNA with the infrequently cutting restriction enzyme MluI. Cloning of MluI fragments in the vector pYAC-RC and one subsequent size fractionation by preparative pulsed-field gel electrophoresis yielded a library with average insert sizes of 600 kb. Ninety-seven percent of the colonies were recombinant. An additional size fractionation of MluI fragments prior to ligation had no significant influence on the size of the YACs. The library currently consists of 5000 clones, which is the equivalent of one human genome. Nineteen percent of the YACs were larger than 1.2 Mb. Since smaller MluI fragments are lost during sizing, they also performed cloning without size fractionation. Only 20% of the colonies were recombinant, probably due ...

1994-05-01

93

Cloning and sequencing of a dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis.

The cellular receptor for murine coronavirus mouse hepatitis virus (MHV)-A59 is a member of the carcinoembryonic antigen (CEA) family of glycoproteins in the immunoglobulin superfamily. We isolated...Full Text Available

1991-12-01

94

Cloning and expression of the gene for the Avi-3 antigen of Mycobacterium avium and mapping of its epitopes.

In Pseudomonas paucimobilis UT26, gamma-hexachlorocyclohexane (gamma-HCH) is converted by two steps of dehydrochlorination to a chemically unstable intermediate, 1,3,4,6-tetrachloro-1,4-cyclohexadiene...Full Text Available

1993-10-01

95

Cloning and expression of portions of the 34-kilodalton-protein gene of Mycobacterium paratuberculosis: its application to serological analysis of Johne's disease.

The Avi-3 antigen, which is found only in Mycobacterium avium culture sonic extracts, is species specific and results in strong skin test activity in guinea pigs sensitized with heat-killed M. avium....Full Text Available

1992-03-01

96

Cloning and Sequencing of a Novel meta-Cleavage Dioxygenase Gene Whose Product Is Involved in Degradation of ?-Hexachlorocyclohexane in Sphingomonas paucimobilis

Paratuberculosis (Johne's disease), an endemic mycobacteriosis of cattle that is caused by Mycobacterium paratuberculosis, is characterized by incoercible diarrhea and fecal shedding of bacteria. The...Full Text Available

1993-04-01

97

Cloning and Sequencing of a 2,5-Dichlorohydroquinone Reductive Dehalogenase Gene Whose Product Is Involved in Degradation of ?-Hexachlorocyclohexane by Sphingomonas paucimobilis

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide,...Full Text Available

1999-11-01

98

Cloning and Nucleotide Sequence Determination of the Entire mec DNA of Pre-Methicillin-Resistant Staphylococcus aureus N315

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide,...Full Text Available

1998-03-01

99

Cloning and Characterization of lin Genes Responsible for the Degradation of Hexachlorocyclohexane Isomers by Sphingomonas paucimobilis Strain B90

In methicillin-resistant Staphylococcus aureus, the methicillin resistance gene mecA is localized within a large chromosomal region which is absent in the methicillin-susceptible...Full Text Available

1999-06-01

100

Cloning and Analysis of a Candida albicans Gene That Affects Cell Surface Hydrophobicity

Hexachlorocyclohexane (HCH) has been used extensively against agricultural pests and in public health programs for the control of mosquitoes. Commercial formulations of HCH consist of a mixture of four...Full Text Available

2002-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

101

Characterization of the H(+)-pumping F1F0 ATPase of Vibrio alginolyticus.

The opportunistic pathogenic yeast Candida albicans exhibits growth phase-dependent changes in cell surface hydrophobicity, which has been correlated with adhesion to host tissues....Full Text Available

2001-06-01

102

Characterization of a Chromosomally Encoded Extended-Spectrum Class A ?-Lactamase from Kluyvera cryocrescens

The F1F0 ATPase of Vibrio alginolyticus was cloned from a chromosomal lambda library. The unc operon, which contains the structural genes for the ATPase, was sequenced and shown to have a gene organization...Full Text Available

1990-12-01

103

Characterization of Two Divergent Lineages of Macaque Rhadinoviruses Related to Kaposi's Sarcoma-Associated Herpesvirus

A chromosomally located β-lactamase gene, cloned and expressed in Escherichia coli from a reference strain of the enterobacterial species Kluyvera cryocrescens,...Full Text Available

2001-12-01

104

Bacterial Artificial Chromosome Mutagenesis Using Recombineering

We have cloned and characterized the entire DNA polymerase gene and flanking regions from Kaposi's sarcoma-associated herpesvirus (KSHV) and two closely related macaque homologs of KSHV, retroperitoneal...Full Text Available

2000-05-01

105

Avian Nephritis Virus (ANV) as a New Member of the Family Astroviridae and Construction of Infectious ANV cDNA

Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. BACs are large enough...Full Text Available

2011-01-01

106

Applications and Experience with PCR-Based Assays to Predict Blood Group Antigens

The complete RNA genome of the avian nephritis virus (ANV) associated with acute nephritis in chickens has been molecularly cloned and sequenced. Excluding the poly(A) tail, the genome comprises 6,927...Full Text Available

2000-09-01

107

A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.

SummaryDNA-based tests are increasingly being used to predict a blood group phenotype. This is possible because genes encoding 29 of the 30 blood group systems have been cloned and sequenced,...Full Text Available

2009-01-01

108

A novel and simple method for construction of recombinant adenoviruses

The single-stranded viral DNA of an M13 phage recombinant containing the early promoter region of SV40 was hybridized with linear, double-stranded replicative form DNA of a related M13 phage containing...Full Text Available

1982-01-01

109

A comparative analysis of phenylpropanoid metabolism, N utilization, and carbon partitioning in fast- and slow-growing Populus hybrid clones

Recombinant adenoviruses have been widely used for various applications, including protein expression and gene therapy. We herein report a new and simple cloning approach to an efficient and robust...Full Text Available

2006-01-01

110

A Marker-Dense Physical Map of the Bradyrhizobium japonicum Genome

The biosynthetic costs of phenylpropanoid-derived condensed tannins (CTs) and phenolic glycosides (PGs) are substantial. However, despite reports of negative correlations between leaf phenolic content...Full Text Available

2009-08-01

111

(+)-Larreatricin hydroxylase, an enantio-specific polyphenol oxidase from the creosote bush (Larrea tridentata)

Bacterial artificial chromosome (BAC) clones are effective mapping and sequencing reagents for use with a wide variety of small and large genomes. This report describes the development of a physical...Full Text Available

2001-08-01

112

Molecular cloning of chicken metallothionein. Deduction of the complete amino acid sequence and analysis of expression using cloned cDNA

An enantio-specific polyphenol oxidase (PPO) was purified ≈1,700-fold to apparent homogeneity from the creosote bush (Larrea tridentata), and its encoding gene was cloned. The...Full Text Available

2003-09-16

113

LHC poster: aerial view of CERN with the LHC sites.

A cDNA library was constructed using RNA isolated from the livers of chickens which had been treated with zinc. This library was screened with a RNA probe complementary to mouse metallothionein-I (MT), and eight chicken MT cDNA clones were obtained. All of the cDNA clones contained nucleotide sequences homologous to regions of the longest (375 bp) cDNA clone. The latter contained an open reading frame of 189 bp, and the deduced amino acid sequence indicates a protein of 63 amino acids of which 20 are cysteine residues. Amino acid composition and partial amino acid sequence analyses of purified chicken MT protein agreed with the amino acid composition and sequence deduced from the cloned cDNA. Amino acid sequence comparison establish that chicken MT shares extensive homology with mammalian MTs. Southern blot analysis of chicken DNA indicates that the chicken MT gene is not a part of a large family of ...

1988-01-25

114

Telomerase-immortalized non-malignant human prostate epithelial cells retain the properties of multipotent stem cells

LHC poster: aerial view of CERN with the LHC sites.

1998-01-01

115

Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21

Understanding prostate stem cells may provide insight into the origin of prostate cancer. Primary cells have been cultured from human prostate tissue but they usually survive only 15-20 population doublings before undergoing senescence. We report here that RC-170N/h/clone 7 cells, a clonal cell line from hTERT-immortalized primary non-malignant tissue-derived human prostate epithelial cell line (RC170N/h), retain multipotent stem cell properties. The RC-170N/h/clone 7 cells expressed a human embryonic stem cell marker, Oct-4, and potential prostate epithelial stem cell markers, CD133, integrin #alpha#2#beta#1"h"i and CD44. The RC-170N/h/clone 7 cells proliferated in KGM and Dulbecco's Modified Eagle Medium with 10% fetal bovine serum and 5 #mu#g/ml insulin (DMEM + 10% FBS + Ins.) medium, and differentiated into epithelial stem cells that expressed epithelial cell markers, including CK5/14, CD44, p63 and cytokeratin 18 ...

2008-01-01

116

Cultivation and utilization of specific wood biomass for synthesis of cellulose based bioethanol

A gene designated BRCA1, implicated in the susceptibility to early-onset familial breast cancer, has recently been localized to chromosome 17q12-q21. To date, the order of DNA markers mapped within this region has been based on genetic linkage analysis. The authors report the use of multicolor fluorescence in situ hybridization to establish a physically based map of five polymorphic DNA markers and 10 cloned genes spanning this region. Three cosmid clones and Alu-PCR-Generated products derived from 12 yeast artificial chromosome clones representing each of these markers were used in two-color mapping experiments to determine an initial proximity of markers relative to each other on metaphase chromosomes. Interphase mapping was then employed to determine the order and orientation of closely spaced loci by direct visualization of fluorescent signals following hybridization of three probes, each detected in a different color. ...

1993-09-01

117

Transformation and radiosensitivity of human diploid skin fibroblasts transfected with activated RAS oncogene and SV40 T-antigen

The energetic characteristics of 6 types of poplar clones cultivated for different pedoclimatic conditions in Romania were determined. Four clones were developed in Italy and 2 in Romania. Five experimental cultures were used to analyze the plant survival rate and biomass production rate. After 2 years of study, the Italian clones were found to have very good adaptability to the pedoclimatic conditions in Romania in comparison with local clones. The Italian clones Monviso and AF-6 registered the most substantial growths and the highest resistance to disease. Bioethanol was synthesized by acidic hydrolysis of the cellulose using 2 approaches. In the first approach the lignocellulosic raw material was hydrolyzed with diluted sulfuric acid at 50 degrees C for 24 hours. After filtration, the solid residue was treated with 30 per cent H{sub 2}SO{sub 4} at 100 degrees C for 6 hours. The ...

2010-07-01

118

Molecular cloning, cDNA sequence, and chromosomal assignment of the human radixin gene and two dispersed pseudogenes

Three normal human diploid cell strains were transfected with an activated Ha-ras oncogene (EJ ras) or SV40 T-antigen. Multiple clones were examined for morphological alterations, growth requirements, ability to grow under anchorage independent conditions, immortality and tumorigenicity in nude mice. Clones expressing SV40 T-antigen alone or in combination with ras protein p21 were significantly radioresistant as compared with their parent cells or clones transfected with the neo gene only. This radioresistant phenotype persisted in post-crisis, immortalized cell lines. These data suggest that expression of the SV40 T-antigen but not activated Ha-ras plays an important role in the radiosensitivity of human diploid cells. The radioresistant phenotype in SV40 T transfected cells was not related to the enhanced level of genetic instability seen in pre-crisis and newly immortalized cells, nor to the process of immortalization ...

1992-08-01

119

From a set of parts to an indivisible whole. Part II: Operations in an open comparative mode

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin. The authors have cloned and sequenced the human radixin cDNA and found the predicted amino acid sequence for the human protein to be nearly identical to those predicted for radixin in the two other species. By Southern analyses of Chinese hamster x human somatic cell hybrid DNA and of PCR products derived from hybrids, the coding gene (RDX) was mapped to 11q. Fluorescence chromosomal in situ hybridization with a cDNA plasmid further localized this gene to band 11q23. However, PCR amplification with [open quotes]radixin-specific[close quotes] primers on the hybrid DNA panel yielded an additional, very similar DNA sequence that was further characterized by direct sequencing of PCR products. This sequence represents a truncated version and ...

1993-04-01

120

Cloning and linkage mapping of three polymorphic tetranucleotide (TAAA)[sub n] repeats on human chromosome 21

This paper describes a new method, HGV2C, for pattern analysis. The HGV2C method involves the construction of a computer ego (CE) based on an individual object that can be either a part of the system under analysis or a newly created object based on a certain hypothesis. The CE provides a capability to analyze data from a specific standpoint, e.g. from a viewpoint of a certain object. The CE is constructed from two identical copies of a query object, and its functioning mechanism involves: a hypothesis-parameter (HP) and infothyristor (IT). HP is a parameter that is introduced into an existing set of parameters. The HP value for one of the clones of a query object is set to equal 1, whereas for another clone it is greater than 1. The IT is based on the previously described algorithm of iterative averaging and performs three functions: 1) computation of a similarity matrix for the group of three objects including two clones ...

2008-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

121

Cloning and expression of a human kidney cDNA for an /alpha//sub 2/-adrenergic receptor subtype

The authors report the cloning, sequencing, and mapping of three short sequence repeat polymorphisms due to tetranucleotide (TAAA) repeats from human chromosome 21. These DNA markers (D21S221, D21S225, D21S226) have been cloned from the chromosome 21-specific plasmid library of J. C. Fuscoe, C. C. Collins, D. Pinkel, and J. W. Gray and were shown to be polymorphic by polymerase chain reaction amplification and polyacrylamide gel electrophoresis. Genotypes were determined in informative CEPH pedigrees and used in linkage analysis relative to other mapped markers on human chromosome 21. One of these markers, D21S221, is closely linked to the amyloid precursor protein gene (APP), which has been implicated in the etiology of familial Alzheimer disease in some families. 18 refs., 3 figs., 2 tabs.

1992-12-01

122

Effect of Apolipoprotein E ?4 on the association between health behaviors and cognitive function in late midlife

An /alpha//sub 2/-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet /alpha//sub 2/-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet /alpha//sub 2/-adrenergic receptor and is consistent with the structure of other members of he family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the /alpha//sub 2/-adrenergic ligand (/sup 3/H)rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the /alpha//sub 2/B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet /alpha//sub 2/-adrenergic receptor (/alpha//sub 2/A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help ...

1988-09-01

123

Use of a trap garden to find additional genetically distinct isolates of the rust fungus Phragmidium violaceum to enhance biological control of European blackberry in Australia

The extent to which the effect of risk factors on cognitive ageing is dependent on APOE ε4 remains unclear. The objective of this study is to examine whether APOE ε4 allele modifies...Full Text Available

124

British Library Electronic Table of Contents (United Kingdom)

Biological control agents can be more effective if their populations are genetically diverse, particularly when the target invasive plant comprises a range of genotypes with different susceptibilities and occurs across various microclimates. We report on the use of an efficient approach to find, in the native range, diverse isolates of a rust fungus for biological control. An outdoor trap garden containing various clones of invasive European blackberry (Rubus fruticosus agg.) collected in Australia, each with a different DNA phenotype, was established in France. Within 4?weeks of establishment, the leaf-rust fungus Phragmidium violaceum was recovered from all clones in the garden. Molecular analyses of eight recovered and purified isolates of the fungus from the garden revealed that they w...

2011-01-01

125

T cell receptor beta chain from sea bream (Sparus aurata): Molecular cloning, expression and modelling of the complexes with MHC class I

British Library Electronic Table of Contents (United Kingdom)

The T cell receptor is a fundamental mediator of the adaptive immune responses, since TR ab on T cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (MHC) on APC cells. In the present study, we report the cloning of six TRB chains cDNA sequences from gilthead sea bream (Sparus aurata), a fish of high economical impact in South Mediterranean aquaculture. The V-BETA domains have the canonical features of known teleost and mammalian TR V-BETA domains and have been divided in four different subgroups. A multiple alignment of the six sea bream TRB chains with other known TRB sequences was assembled and showed the conservation of the four cysteine residues involved in disulphide bonds and of some amino acids with an importan...

2008-01-01

126

Identification of prostate cancer antigens by automated high-throughput filter immunoscreening

British Library Electronic Table of Contents (United Kingdom)

There is a need for earlier and more accurate cancer diagnostics as well as new targets for cancer immunotherapy. To this end, it is important to identify sets of tumour antigens specific for different cancer forms. Several methods that identify potential tumour antigens in an arrayed and high-throughput format have been developed during the last years of SEREX (serological identification of antigens by recombinant expression cloning) related research. Such techniques may hold the potential to describe the complete immunogenic part of the cancer proteome, also called the cancer immunoproteome.We have developed a powerful platform for automated serological high-throughput filter screening of tumour cDNA libraries. The screening format of this method is 18,000 single cDNAs clones, which is s...

2008-01-01

127

Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

British Library Electronic Table of Contents (United Kingdom)

A 4.1?kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen Diaporthe perjuncta. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different Diaporthe spp. The transfected species included three D. ambigua isolates and an unidentified Phomopsis asexual state of a Diaporthe sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DR...

2011-01-01

128

Consensus sequence L/PKSSLL mimics crucial epitope on Loop III of Taiwan cobra cardiotoxin

British Library Electronic Table of Contents (United Kingdom)

Phage display is effective in screening peptides that mimic venom's neutralizing epitopes. A phage display cyclized heptapeptide library (C7C library) was panned with purified divalent antivenin IgG, which neutralizes Naja naja atra venom (NAV) and Bungarus multicinctus venom (BMV). The selected heptapeptide sequences were aligned with known protein sequences of NAV and BMV in GenBank. One of the four consensus sequences, L/PKSSLL, mimicked the crucial epitope on Loop III of Taiwan cobra cardiotoxin that is associated with the venom's lethal potency. In dot blot analysis, several clones showed varying reactivities for NAV monovalent antivenin and lesser cross-reactions with BMV monovalent antivenin. The KSSLLRN-carrying phage occurred four times in selected clones and showed the strongest ...

2009-01-01

129

Cloning and characteristics of Brn1 gene in Curvularia lunata causing leaf spot in maize

British Library Electronic Table of Contents (United Kingdom)

The full length cDNA of the Brn1 was first cloned, and then expression of the Brn1 was analyzed and the function was identified by silencing technology. Results show that the full length cDNA of the C. lunata Brn1 gene contains 1001 base pairs and an 801?bp open reading frame encoding 267 amino acids. Semi-quantitative PCR analysis shows that the expression of Brn1 at 96?h is significantly higher than at 24 and 72?h (p?Brn1-silenced transformants were light brown in culture filtrate, and have significantly reduced toxin production relative to the wild-type. These results imply that Brn1 gene in C. lunata is not only involved in 1,8-dihydroxynaphthalene melanin synthesis, but is also relatively associated with toxin biosynthesis of the pathogen.

2011-01-01

130

A New Stem Taper Function for Short-rotation poplar

Mixed waste disposal facility at the Nevada Test Site

A new stem taper function was established for individual trees of two poplar hybrid clones grown on a short-rotation coppice. The model could be easily fitted and required three parameters to be estimated. It can be used to estimate both diameter at a given height and height for a given top diameter. Two of the three parameters controlled the conical and the neiloid parts of the stem. Significant differences in these parameters were observed between the two clones even if no differences were observed for diameter at breast height or total height of the stem. The model could not be integrated to calculate volumes (total volume, merchantable volume), which were estimated by numerical integration. However, use of this new model allows the optimal length of billets to be determined and thus maximizes the merchantable biomass of poplar in short-rotation coppice by minimizing the biomass of residues.

2003-07-01

131

Site requirements for ITER

This report provides a brief overview of the history and planning for a proposed mixed waste disposal site on the Nevada Test Site. 8 figs.

1987-01-01

132

Site requirements for ITER

In this paper, the compulsory site requirements and the site design assumptions which are being used for the present ITER plant are briefly described. A set of different parameters from the site design assumptions is also described, which was adopted as an additional exercise for cost sensitivity analysis of the ITER plant for these parameters. (orig.) 1 refs.

1998-09-01

133

Cookie same origin policy

In this paper, the compulsory site requirements and the site design assumptions which are being used for the present ITER plant are briefly described. A set of different parameters from the site design assumptions is also described, which was adopted as an additional exercise for cost sensitivity analysis of the ITER plant for these parameters. (orig.)

1998-09-01

134

Archaeoseismological studies on historical heritage sites in the Gyeongju area, SE Korea

Basic cross site scripting attacks

2009-02-03

135

cDNA Cloning of the Basement Membrane Chondroitin Sulfate Proteoglycan Core Protein, Bamacan: A Five Domain Structure Including Coiled-Coil Motifs

Not Available

2011-10-01

136

Tissue culture process for the clonal production of loblolly pine plantlets

Basement membranes contain several proteoglycans, and those bearing heparan sulfate glycosaminoglycans such as perlecan and agrin usually predominate. Most mammalian basement membranes also contain...Full Text Available

1997-01-27

137

The normal developmental regulation of a cloned sgs3 'glue' gene chromosomally integrated in Drosophila melanogaster by P element transformation.

A tissue culture process, organogenesis, is described which involves the stepwise production of a shoot apex, which grows to have leaves and stem and finally roots, culminating in the production of a complete plant. The production and subsequent development of numerous adventitious shoots, preferably from adult tree tissues offers the greatest potential for commercial cloned plantlet production. The process describes these steps for Pinus taeda, the loblolly pine. (Refs. 25).

1981-02-01

138

The development of in vitro mutagenicity testing systems using T-lymphocytes

A 7-kb genomic segment containing the coding sequence for the Drosophila melanogaster Formosa variant of salivary gland secretion protein 3 (sgs3) has been inserted into the snw y, bw, st strain of...Full Text Available

1983-01-01

139

The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species.

This annual report describes progress in studies on hprt mutations induced by radon or Indium 111 along with the corresponding mutation frequency, cloning and molecular spectra in human T-lymphocytes. Parallel studies on the mutation susceptibility between individuals is being investigated by hprt mutation studies on ataxia telangiectasia and xeroderma pigmentosum.

1993-05-01

140

Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-?-bisabolene synthase from grand fir (Abies grandis)

A recombinant M13 clone (O42) containing a 65 b.p. cDNA fragment from human fetal liver mRNA coding for glyceraldehyde-3-phosphate dehydrogenase has been identified and it has been used to isolate from...Full Text Available

1984-12-11

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

141

Sequence and Organization of pXO1, the Large Bacillus anthracis Plasmid Harboring the Anthrax Toxin Genes

(E)-α-Bisabolene synthase is one of two wound-inducible sesquiterpene synthases of grand fir (Abies grandis), and the olefin product of this cyclization reaction...Full Text Available

1998-06-09

142

Quox-1, a quail homeobox gene expressed in the embryonic central nervous system, including the forebrain.

The Bacillus anthracis Sterne plasmid pXO1 was sequenced by random, “shotgun” cloning. A circular sequence of 181,654 bp was generated. One hundred forty-three open...Full Text Available

1999-10-01

143

Quantum Cloning for Absolute Radiometry

This paper reports the cloning and sequencing of a quail homeobox-containing gene, Quox-1, and its expression pattern in embryos from 3 to 6 days (E3 to E6) of development as determined by in situ hybridization....Full Text Available

1991-03-15

144

Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes

In the quantum regime information can be copied with only a finite fidelity. This fidelity gradually increases to 1 as the system becomes classical. In this Letter we show how this fact can be used to directly measure the amount of radiated power. We demonstrate how these principles can be used to build a practical primary standard.

2010-08-20

145

In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their...Full Text Available

2009-12-01

146

Molecular methods

Molecular cloning of the N-terminus of GTBP

Chapter 5, describes some of the most important molecular methods used in the study of chromosome structure and function. The methods discussed include fragmentation of DNA, cloning, flow cytometry and chromosome sorting, is situ hybridization, polymerase chain reaction (PCR), and yeast artificial chromosomes (YACs). 18 refs., 3 figs., 1 tab.

1993-12-31

147

Microsomal Electron Transfer in Higher Plants: Cloning and Heterologous Expression of NADH-Cytochrome b₅ Reductase from Arabidopsis

Defects in mismatch repair genes cause the genetic instability characteristic of hereditary nonpolyposis colorectal cancer and a subset of sporadic colon tumors. The newest member of the mismatch repair gene family, GTBP, has recently been identified as a partial cDNA. Here, we describe the isolation of its 5{prime} terminus, allowing definition of the entire coding region. Several polymorphisms within the 5{prime} end were identified and are presented. 13 refs., 1 fig.

1996-02-01

148

Microbial dynamics in upflow anaerobic sludge blanket (UASB) bioreactor granules in response to short-term changes in substrate feed

AtCBR, a cDNA encoding NADH-cytochrome (Cyt) b₅ reductase, and AtB5-A and AtB5-B, two cDNAs encoding Cyt b₅, were isolated from Arabidopsis....Full Text Available

1999-01-01

149

In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sludges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I

The complexity and diversity of the microbial communities in biogranules from an upflow anaerobic sludge blanket (UASB) bioreactor were determined in response to short-term changes in substrate feeds. The reactor was fed simulated brewery wastewater (SBWW) (70% ethanol, 15% acetate, 15% propionate) for 1.5 months (phase 1), acetate / sulfate for 2 months (phase 2), acetate-alone for 3 months (phase 3), and then a return to SBWW for 2 months (phase 4). Performance of the reactor remained relatively stable throughout the experiment as shown by COD removal and gas production. 16S rDNA, methanogen-associated mcrA and sulfate reducer-associated dsrAB genes were PCR amplified, then cloned and sequenced. Sequence analysis of 16S clone libraries showed a relatively simple community composed mainly of the methanogenic Archaea (Methanobacterium and Methanosaeta), members of the Green Non-Sulfur (Chloroflexi) group of Bacteria, followed by fewer numbers ...

2010-08-01

150

Hybridization with synthetic oligonucleotides

We previously showed that very thin filamentous bacteria affiliated with the division green non-sulfur bacteria were abundant in the outermost layer of thermophilic methanogenic sludge granules fed...Full Text Available

2001-12-01

151

High Levels of Tryptamine Accumulation in Transgenic Tobacco Expressing Tryptophan Decarboxylase ¹

Procedures are described for the use of synthetic oligonucleotides for Southern blot experiments and gene bank screening, and the effect of various mismatches on the efficiency of hybridization is demonstrated. The following topics are discussed: sensitivity vs. specificity, hybridization of a 12-mer to the lambda endolysin gene; hybridization of oligonucleotide probes to the E. coli lac operator; hybridization of synthetic probes to the CYC1 gene of yeast; and cloning eucaryotic genes. (HLW)

1978-01-01

152

Features of two hepatitis B virus (HBV) DNA integrations suggest mechanisms of HBV integration.

A full-length complementary DNA clone encoding tryptophan decarboxylase (TDC; EC 4.1.1.28) from Catharanthus roseus (De Luca V, Marineau C, Brisson N [1989] Proc Natl Acad Sci USA 86:...Full Text Available

1990-11-01

153

Two integrated hepatitis B virus (HBV) DNA molecules were cloned from two primary hepatocellular carcinomas each containing only a single integration. One integration (C3) contained a single linear...Full Text Available

1989-06-01

154

DNA repair genes

Cloning, sequence analysis and over-expression of the gene for the class II fructose 1,6-bisphosphate aldolase of Escherichia coli.

Fission yeast S. pombe is assumed to be a good model for cloning of human DNA repair genes, because human gene is normally expressed in S. pombe and has a very similar protein sequence to yeast protein. We have tried to elucidate the DNA repair mechanisms of S. pombe as a model system for those of mammals. (J.P.N.)

1995-12-01

155

Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase gene involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis.

Nucleotide sequence analysis of the Escherichia coli chromosomal DNA inserted in the plasmid pLC33-5 of the Clarke and Carbon library [Clarke & Carbon (1976) Cell 9, 91-99] revealed the existence...Full Text Available

1989-01-15

156

Chromosome-Encoded Ambler Class A ?-Lactamase of Kluyvera georgiana, a Probable Progenitor of a Subgroup of CTX-M Extended-Spectrum ?-Lactamases

In Pseudomonas paucimobilis UT26, gamma-hexachlorocyclohexane (gamma-HCH) is converted to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-DDOL), which is then metabolized to 2,5-dichlorohydroquinone....Full Text Available

1994-06-01

157

Analysis of the murine All-1 gene reveals conserved domains with human ALL-1 and identifies a motif shared with DNA methyltransferases.

A chromosome-encoded β-lactamase gene, cloned and expressed in Escherichia coli from Kluyvera georgiana reference strain CUETM 4246-74 (DSM 9408), encoded the...Full Text Available

2002-12-01

158

Aerial picture of BA4 at SPS (LHC T18 building site).

A series of translocation break points found in a subset of human acute leukemias have one of the breaks on human chromosome 11q23. This region has recently been cloned and a large gene, ALL-1, with...Full Text Available

1993-07-01

159

Sequence of Potentials Lying Between the U(5) and X(5) Symmetries

Aerial picture of BA4 at SPS (LHC T18 building site).

1999-01-01

160

Ozone exposure triggers the emission of herbivore-induced plant volatiles, but does not disturb tritrophic signalling

Starting from the original collective Hamiltonian of Bohr and separating the beta and gamma variables as in the X(5) model of Iachello, an exactly soluble model corresponding to a harmonic oscillator potential in the beta-variable (to be called X(5)-$\\beta^2$) is constructed. Furthermore, it is proved that the potentials of the form $\\beta^{2n}$ (with n being integer) provide a ``bridge'' between this new X(5)-$\\beta^2$ model (occuring for n=1) and the X(5) model (corresponding to an infinite well potential in the beta-variable, materialized for n going to infinity. Parameter-free (up to overall scale factors) predictions for spectra and B(E2) transition rates are given for the potentials $\\beta^2$, $\\beta^4$, $\\beta^6$, $\\beta^8$, corresponding to E(4)/E(2) ratios of 2.646, 2.769, 2.824, and 2.852 respectively, compared to the E(4)/E(2) ratios of 2.000 for U(5) and 2.904 for X(5). Hints about ...

2004-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

161

cDNA cloning and sequence analysis of preproendothelin-1 (PPET-1) from salmon, Oncorhynchus keta.

We evaluated the similarities between ozone-induced and mite-induced emission of volatile organic compounds (VOCs) from lima beans, and tested the response of the natural enemies of herbivores to these emissions using trophic system of two-spotted spider mites and predatory mites. The acute ozone-exposure and spider mite-infestation induced the emission of two homoterpenes, (E)-4,8-dimethyl-1,3,7-nonatriene and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene, and (Z)-3-hexenyl acetate. Only plants with spider mite-infestation emitted the monoterpene (E)-{beta}-ocimene. Predatory mites were equally attracted to ozone-exposed and unexposed plants, but discriminated between spider mite-infested and uninfested plants, when both were exposed to ozone. The similarities between ozone and herbivore-induced VOCs suggest that plant defence against phytotoxic ozone and the production of VOCs for attraction of the ...

2004-09-01

162

cDNA Cloning and mRNA analysis of PGC-1 in epitrochlearis muscle in swimming-exercised rats.

The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 (26%, 29%, 24%, and 39%, respectively). PMID:16740985

2006-06-01

163

The mouse formin (Fmn) gene: Genomic structure, novel exons, and genetic mapping

Peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1), a cold-inducible coactivator of nuclear receptors, stimulates mitochondrial biogenesis and respiration in muscle cells. In the present study, we first cloned a rat PGC-1 gene from a brown adipose tissue cDNA library which encodes a predicted 796-amino-acid protein and exhibits respectively 98% and 95% identity with the mouse and human homologues. Next, we examined the effect of swimming exercise training on the level of expression of the PGC-1 gene in rat epitrochlearis (Epi) muscle. PGC-1 mRNA level in Epi muscle in rats that swam 2 h a day for 3 and 7 days increased dramatically by 154% and 163%, respectively, compared to the non-exercised control group. PGC-1 mRNA up-regulation was not observed in an immersion group treated at 35 degrees C during the training program but without swimming exercise. These results demonstrate that expression of the PGC-1 gene in Epi muscle is induced not only ...

2000-08-01

164

Smooth muscle myosin heavy chain locus (MYH11) maps to 16p13. 13-p13. 12 and establishes a new region of conserved synteny between human 16p and mouse 16

Mutations in the mouse formin (Fmn) gene, formerly known as the limb deformity (ld) gene, give rise to recessively inherited limb deformities and renal malformations or aplasia. The Fmn gene encodes many differentially processed transcripts that are expressed in both adult and embryonic tissues. To study the genomic organization of the Fmn locus, we have used Fmn probes to isolate and characterize genomic clones spanning 500 kb. Our analysis of these clones shows that the Fmn gene is composed of at least 24 exons and spans 400 kb. We have identified two novel exons that are expressed in the developing embryonic limb bud as well as adult tissues such as brain and kidney. We have also used a microsatellite polymorphism from within the Fmn gene to map it genetically to a 2.2-cM interval between D2Mit58 and D2Mit103. 36 refs., 6 figs., 1 tab.

1997-02-01

165

Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32. 1

The human smooth muscle myosin heavy chain locus (MYH11) was mapped by fluorescence in situ hybridization to the middle of the p arm of chromosome 16 using a genomic cosmid clone containing coding sequences of the gene as probe. Probe from coding sequence, when applied to Southern blots of a panel of hybrids containing different portions of human chromosome 16, localized the gene to 16p13.13-13.12. Coding sequence PCR primers, when used on the DNA from a CHO-mouse hybrid clone mapping panel informative for mouse chromosomes, showed that the gene was located on mouse chromosome 16. These results correct a recent assignment of MYH11 from 16q12.2 to the region of the 16p-arm inversion breakpoint seen in acute myelomonocytic leukemia (AMML) M4Eo and demonstrate that the conflicting data do not result from the presence of additional MYH genes on the q arm of the chromosome. Also, a new region of conserved synteny between human 16p and mouse 16 is ...

1993-10-01

166

Mapping of quantitative trait loci (QTL) for production, resistance and tolerance traits in Salix. Final Report

Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.

1994-05-15

167

Identification of three related human GRO genes encoding cytokine functions

Quantitative trait loci (QTL) for growth traits, water use efficiency and tolerance/resistance against metals and herbivores have been identified. A hybrid F2 population originating from a cross between a Salix dasyclados-clone (SW901290) and a S. viminalis-clone ('Jorunn') was used for the different studies in this project. The growth response was analyzed in a greenhouse experiment with two water treatments, normal and drought. In addition, three field experiments with contrasting soils and climates were established. QTL specific for each treatment or field environment but also QTL stable over the treatments or field environments were detected. Each QTL explained from 8 to 29 % of the phenotypic variation depending on trait, treatment or field environment. Clusters of QTL for different traits were mapped indicating a common genetic base or tightly-linked QTL. Stable QTL identified for dryweight can be useful tools for early ...

2004-05-01

168

Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy

The product of the human GRO gene is a cytokine with inflammatory and growth-regulatory properties; GRO is also called MGSA for melanoma growth-stimulatory activity. The authors have identified two additional genes, GRO#beta# and GRO#gamma#, that share 90% and 86% identity at the deduced amino acid level with the original GRO#alpha# isolate. One amino acid substitution of proline in GRO#alpha# by leucine in GRO#beta# and GRO#gamma# leads to a large predicted change in protein conformation. Significant differences also exist in the 3' untranslated region, including different numbers of ATTTA repeats associated with mRNA instability. A 122-base-pair region in the 3' region is conserved among the three GRO genes, and a part of it is also conserved in the Chinese hamster genome, suggesting a role in regulation. DNA hybridization with oligonucleotide probes and partial sequence analysis of the genomic clones confirm that the three forms are derived from related but ...

169

First report of the complete sequence of Sida golden yellow vein virus from Jamaica.

The Drosophila hairy gene encodes a basic helix- loop-helix protein that functions in at least two steps during Drosophila development: (1) during embryogenesis, when it partakes in the establishment of segments, and (2) during the larval stage, when it functions negatively in determining the pattern of sensory bristles on the adult fly. In the rat, a structurally homologous gene (RHL) behaves as an immediate-early gene in its response to growth factors and can, like that in Drosophila, suppress neuronal differentiation events. Here, the authors report the genomic cloning of the human hairy gene homolog (HRY). The coding region of the gene is contained within four exons. The predicted amino acid sequence reveals only four amino acid differences between the human and rat genes. Analysis of the DNA sequence 5[prime] to the coding region reveals a putatitve untranslated exon. To increase the value of the HRY gene as a genetic marker and to assess its potential ...

1994-03-01

170

Evidence of minimal methanogenic numbers and activity in sediments collected from the JAPEX/JNOC/GSC et al. Mallik 5L-38 gas hydrate production research well

Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, Cuba and Florida in ...

2011-05-29

171

Cloning and mapping of the mouse {alpha}7-neuronal nicotinic acetylcholine receptor

Gene analysis was used to determined the presence, abundance and phylogenetic affiliation of methanogens that exist in gas-hydrate-bearing sediment samples obtained from 23 drill cores from the JAPEX/JNOC/GSC et al. Mallik 5L-38 gas hydrate research well. Rates of methane production were examined using sediment-inoculated enrichments containing {sup 14}C-labeled carbon substrates, carbon dioxide and acetate. Archaeal 16S rDNA was only detected in 6 of the samples, resulting in 8 sequences with relationships to the Miscellaneous Crenarchaeotic Group (7 clones) and the Subsurface Euryarchaeotic Group (1 clone). The single Euryarchaeota sequence did not appear to be related to methanogens. Subsamples from the cores showed variable results upon DNA extraction and amplification. Methanogenic Coenzyme M (CoM) was detected in 13 of the 20 cores, but methanogenic methyl CoM reductase genes were not amplified from the samples when using a sensitive ...

2005-07-01

172

Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID.

We report the isolation of cDNA clones for the mouse {alpha}7 neuronal nicotinic acetylcholine receptor subunit (gene symbol Acra7), the only nicotinic receptor subunit known to bind a-bungarotoxin in mammalian brain. This gene may have relevance to nicotine sensitivity and to some electrophysiologic findings in schizophrenia. The mouse {alpha}7 subunit gene encodes a protein of 502 amino acids with substantial identity to the rat (99.6%), human (92.8%), and chicken (87.5%) amino acid sequences. The {alpha}7 gene was mapped to mouse chromosome 7 near the p locus with the following gene order from proximal to distal: Myod1-3.5 {+-}1.7 cM-Gas2-0.9 cM {+-} 0.9 cM-D7Mit70-1.8 {+-} 1.2 cM- Acra7-4.4 {+-}1.0 cM-Hras1-ps11/Igf1r/Snrp2a. The human gene was confirmed to map to the homologous region of human chromosome 15q13-q14. 26 refs., 3 figs.

1995-03-20

173

A catalase from the freshwater mussel Cristaria plicata with cloning, identification and protein characterization

Regulation of transcription initiation by RNA polymerase II requires TFIID, a multisubunit complex composed of the TATA binding protein (TBP) and at least seven tightly associated factors (TAFs). Some TAFs act as direct targets or coactivators for promoter-specific activators while others serve as interfaces for TAF-TAF interactions. Here, we report the molecular cloning, expression and characterization of Drosophila dTAFII60 and its human homolog, hTAFII70. Recombinant TAFII60/70 binds weakly to TBP and tightly to the largest subunit of TFIID, TAFII250. In the presence of TAFII60/70, TBP and TAFII250, a stable ternary complex is formed. Both the human and Drosophila proteins directly interact with another TFIID subunit, dTAFII40. Our findings reveal that Drosophila TAFII60 and human TAFII70 share a high degree of structural similarity and that their interactions with other subunits of TFIID are conserved.Images

1993-12-15

174

British Library Electronic Table of Contents (United Kingdom)

Catalase is an important antioxidant protein which can protect organisms against various oxidative stresses by eliminating hydrogen peroxide. The catalase cDNA of Cristaria plicata@?cpCAT@? was cloned from the haemocytes using degenerate primers by the method of 3' and 5' rapid amplification of cDNA ends PCR. The gene is 4863 bp long and has a total of two introns and three exons. The precise size and location of the introns and exons have been determined. In addition the full-length cDNA of cpCAT contained 2618 bp, The cDNA contained a 5' untranslated region (UTR) of 136 nucleotides, the 3' UTR of 979 bp with a canonical polyadenylation signal sequence AATAAA and a polyA tail, and an open reading frame (ORF) of 1503 bp, encoding 501 amino acid residues with 56.86 kDa predicted molecular w...

2011-01-01

175

Propagation of jarrah (Eucalyptus marginata) by organ and tissue culture

Methods to improve the fertility of poinsettia

Methods are described for the production of callus and subsequent shoot and root induction and multiplication using stamen filaments, nodes of young stems of mature trees or cotyledon petioles. A high frequency of contamination occurred using nodal sections from mature trees, whereas up to 100% sterility was obtained with cotyledons and stamens. The rooting % of shoot cultures from nodes or stamen callus was low compared with that of shoots from seedling callus. Considerable variation was observed between clones from different trees in their ability to produce shoots and roots. 27 references

1982-01-01

176

British Library Electronic Table of Contents (United Kingdom)

`Success' or `Red Splendor' plants propagated by shoot tip culture or embryogenesis had higher pollen germination than those propagated by cutting. All clones of `Pepride', `Peter Star' and `Nobel Star' propagated by cutting or by embryogenesis or shoot tip culture did not have viable pollen. `Peter Star' mutants through mutagenesis could recover pollen viability. When both parents were propagated by tissue culture, fruit set increased compared to when one or both parents were propagated by cuttings. Furthermore, the number of the harvested seeds doubled per pollination set, when the tissue culture parents were used instead of cutting-propagation plants.

2008-01-01

177

Clones to replace forest seedlings

CLEO: a knowledge-based refueling assistant at FFTF

A considerable time can elapse between initial selection of candidate plus trees and harvest of improved seed. The technique showing the greatest promise of shortening this interval is vegetative propagation. Stock plants are grown for two years from seed before the first propagation cycle begins and each plant can be bulked-up 300-500 times over two cycles. An initial stock of 2500 superior Sitka Spruce plants can be multiplied to yield between 300,000 and 750,000 rooted cuttings for forest use within four years from the start of propagation.

1985-01-01

178

Selected Web Resources | Natural Hazards Center

A computer software system, CLEO, is used to assist in the planning and performance of the reactor refueling operations at the Fast Flux Test Facility (FFTF). It is a recently developed application of artificial intelligence software with both expert systems and automated reasoning aspects. CLEO, an acronym for Cloned LEO, is a logic-based computer program written in Pascal. It imitates the processes that the refueling expert for FFTF performs in organizing the refueling of FFTF. The computer assistant seeks to organize the sequence of core component movements according to the rules and logic used by the expert. In this form, CLEO has aspects that tie it to both the expert systems and automated reasoning areas within the artificial intelligence field.

1985-11-10

179

Remedial action and site design for stabilization of the inactive uranium mill tailings sites at Slick Rock, Colorado. Attachment 2, Geology report

of Colorado at Boulder Search This Site Natural Hazards Observer Disaster Research CU Web Site CU People go Search This Site Natural Hazards Observer Disaster Research CU Web...

2011-10-08

180

Receptor Binding Sites and Antigenic Epitopes on the Fiber Knob of Human Adenovirus Serotype 3

This report presents geologic considerations that are pertinent to the Remedial Action Plan for Slick Rock mill tailings. Topics covered include regional geology, site geology, geologic stability, and geologic suitability.

1993-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

181

Hanford Site environmental report for calendar year 1989

The adenovirus fiber knob causes the first step in the interaction of adenovirus with cell membrane receptors. To obtain information on the receptor binding site(s), the interaction of labeled cell...Full Text Available

1998-11-01

182

Correct implementation of the Argonne QuickSite{sup SM} process for preremedial site investigations

This report is a summary of the environmental status of the Hanford Site in 1989. It includes descriptions of the Site and its mission, the status of compliance with environmental regulations, planning and activities to accomplish compliance, environmental protection and restoration activities, and environmental monitoring. 97 refs., 67 figs., 14 tabs.

1990-05-01

183

Molecular cloning, characterization, and expression of human ADP-ribosylation factors: Two guanine nucleotide-dependent activators of cholera toxin

Expedited site characterization (ESC), developed by Argonne National Laboratory, is an interactive, integrated process emphasizing the use of existing data of sufficient quality, multiple complementary characterization methods, and on-site decision making to optimize environmental site investigations. The Argonne ESC is the basis for the provisional ESC standard guide of the ASTM (American Society for Testing and Materials). QuickSite{sup SM} is the implementation package developed by Argonne to facilitate ESC of sites contaminated with hazardous wastes. At various sites, Argonne has successfully implemented QuickSite{sup SM} and demonstrated the technical superiority of the ESC process over traditional methodologies guided by statistics and random-sampling approaches. A key feature in the success of QuickSite{sup SM} ...

1997-10-01

184

Cloning of the cDNA for the human. beta. /sub 1/-adrenergic receptor

ADP-ribosylation factors (ARFs) are small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin. Two ARF cDNAs, ARF1 and ARF3, were cloned from a human cerebellum library. Based on deduced amino acid sequences and patterns of hybridization of cDNA and oligonucleotide probes with mammalian brain poly(A)"+ RNA, human ARF1 is the homologue of bovine ARF1. Human ARF3, which differs from bovine ARF1 and bovine ARF2, appears to represent a newly identified third type of ARF. Hybridization patterns of human ARF cDNA and clone-specific oligonucleotides with poly(A)"+ RNA are consistent with the presence of at least two, and perhaps four, separate ARF messages in human brain. In vitro translation of ARF1, ARF2, and ARF3 produced proteins that behaved, by SDS/PAGE, similar to a purified soluble brain ARF. Deduced amino acid sequences of human ARF1 and ARF3 contain regions, similar to those in other G proteins, that ...

1989-01-01

185

Waste generation and pollution prevention progress fact sheet: Savannah River Site

Screening of a human placenta lambdagt11 library has led to the isolation of the cDNA for the human ..beta../sub 1/-adrenergic receptor (..beta../sub 1/AR). Used as the probe was the human genomic clone termed G-21. This clone, which contains an intronless gene for a putative receptor, was previously isolated by virtue of its cross hybridization with the human ..beta../sub 2/-adrenergic receptor (..beta../sub 2/AR). The 2.4-kilobase cDNA for the human ..beta../sub 1/AR encodes a protein of 477 amino acid residues that is 69% homologous with the avian ..beta..AR but only 54% homologous with the human ..beta../sub 2/AR. This suggests that the avian gene encoding ..beta..AR and the human gene encoding ..beta../sub 1/AR evolved from a common ancestral gene. RNA blot analysis indicates a message of 2.5 kilobases in rat tissues, with a pattern of tissue distribution consistent with ..beta../sub 1/AR binding. This pattern is quite distinct from the ...

1987-11-01

186

The sites and topology of mitochondrial superoxide production

This Fact Sheet provides the mission of the Savannah River Site and information on 1994 pollution prevention and recycling accomplishments.

1994-12-31

187

Studies on the acidity of mordenite and ZSM-5. 2. Loss of Broensted acidity by dehydroxylation and dealumination

Mitochondrial superoxide production is an important source of reactive oxygen species in cells, and may cause or contribute to ageing and the diseases of ageing. Seven major sites of superoxide...Full Text Available

2010-08-01

188

State Cancer Profiles Home Page

Quantitative determination of Broensted acid site concentrations in calcined H-mordenite and H-ZSM-5 by means of conductometric titration and infrared spectroscopy reveals that the concentration of Broensted acid sites present is lower than would be expected on the basis of the aluminum content. This discrepancy is attributed to dealumination and dehydroxylation processes occurring during calcination. A model is proposed according to which the degree of these processes strongly depends on the concentration of vicinal aluminum T sites in the zeolite: Broensted acid sites associated with these T sites are lost, and the remaining Broensted acidity is related to the concentration of the (remaining) isolated aluminum T sites. This model quantitatively correlates the experimentally determined concentration of Broensted acid sites with the ...

1993-01-14

189

Public Health, Commissioned Corps

Washington West Virginia Wisconsin Wyoming Cancer --- Choose a Cancer Site --- All Cancer Sites Bladder Brain & ONS Breast (Female) Breast (Female in situ) Cervix Childhood...

2011-10-15

190

Geologic setting of the New Production Reactor within the Savannah River Site

may experience difficulty opening the Facebook page due to current HHS policy and network security that blocks access to the site. Site Map - Contact Us - Linking to USPHS.gov -...

2011-10-16

191

The number and metallicities of the most metal-poor stars

The geology and hydrology of the reference New Production Reactor (NPR) site at Savannah River Site (SRS) have been summarized using the available information from the NPR site and areas adjacent to the site, particularly the away from reactor spent fuel storage site (AFR site). Lithologic and geophysical logs from wells drilled near the NPR site do not indicate any faults in the upper several hundred feet of the Coastal Plain sediments. However, the Pen Branch Fault is located about 1 mile south of the site and extends into the upper 100 ft of the Coastal Plain sequence. Subsurface voids, resulting from the dissolution of calcareous portions of the sediments, may be present within 200 ft of the surface at the NPR site. The water table is located within 30 to 70 ft of the surface. The NPR ...

1991-12-31

192

Chemical resistance, void content and tensile properties of oil palm/jute fibre reinforced polymer hybrid composites

Simple, one-zone models for inhomogeneous chemical evolution of the Galactic halo are used to predict the number fraction of zero-metallicity, Population III stars, which currently is empirically estimated at < 4e-4. These analytic models minimize the number of free parameters, highlighting the most fundamental constraints on halo evolution. There are disagreements of at least an order of magnitude between observations and predictions in limiting cases for both homogeneous Simple Model and Simple Inhomogeneous Model (SIM). Hence, this demonstrates a quantitative, unambiguous discrepancy in the observed and expected fraction of Population III stars. We explore how the metallicity distribution of the parent enrichment events f(z_0) drives the SIM and predictions for the Population III fraction. The SIM shows that the previously-identified "high halo" and "low halo" populations are consistent with a continuous evolutionary progression, and ...

2003-01-01

193

British Library Electronic Table of Contents (United Kingdom)

Tri layer hybrid composites of oil palm empty fruit bunches (EFB) and jute fibres was prepared by keeping oil palm EFB as skin material and jute as the core material and vice versa. The chemical resistance, void content and tensile properties of oil palm EFB/Jute composites was investigated with reference to the relative weight of oil palm EFB/Jute, i.e. 4:1, the fibre loading was optimized and different layering pattern were investigated. It is found from the chemical resistance test that all the composites are resistant to various chemicals. It was observed that marked reduction in void content of hybrid composites in different layering pattern. From the different layering pattern, the tensile properties were slightly higher for the composite having jute as skin and oil palm EFB as core ...

2011-01-01

194

A polarized neutron reflectometry study of the spin glass freezing in a 29 nm thick AuFe film

Studies on Flora of Wetlands on Jeju City for Application as Nature Exploration Sites

We performed polarized neutron reflectometry (PNR) experiments on a 29 nm thick Au{sub 93}Fe{sub 7} film in a temperature range from 295 K down to 2 K in a vertical magnetic field up to 6 T. These high-field experiments were performed on the C5 spectrometer in Chalk River, Canada, using a split-pair cryomagnet. The magnetization as determined by PNR can be described with a Brillouin function from 295 K down to 50 K assuming the magnetic moment of isolated Fe atoms, i.e. 4{mu}{sub B} per Fe atom. Below 50 K the onset of the spin-glass freezing is observed as a strong deviation from this Brillouin type behavior of isolated atoms.

2007-07-15

195

Radionuclides Migration Prevention

Full Text Available

2009-04-01

196

International Science & Technology Center (ISTC)

Prevention of Radionuclide Migration from the Soil of Semipalatinsk Nuclear Test Site by Using of Interpolymer Complexes

197

Radiation assessment of contaminated sites of peat mining and peat bricks used as fuel by the population of Zhitomir area of the Ukraine

Quality Assurance of Intensity Modulated Radiation Therapy: Site-Specific Results of Eulji University Hospital

Short communication.

198

Full Text Available

2011-06-01

199

Pseudogibbula duponti

Potential Hazards from Neutrino Radiation at Muon Colliders

... 1970s from two different sites on the River Matadi. It is likely to be restricted to a ... ...

200

On-site burning, remote camp

High energy muon colliders, such as the TeV-scale conceptual designs now being considered, are found to produce enough high energy neutrinos to constitute a potentially serious off-site radiation hazard in the neighbourhood of the accelerator site. A general characterization of this radiation hazard is given, followed by an order-of-magnitude calculation for the off-site annual radiation dose and a discussion of accelerator design and site selection strategies to minimize the radiation hazard.

1999-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

201

Ocean disposal feasibility study: candidate DOE (FUSRAP) soil characterization. [Formerly Utilized Sites Remedial Action Program

... wood, kitchen wastes, and human faeces. The ash from such waste burning shall be deposited and ...

202

NASA Technical Reports Server - Magnetic forming coil design and ...

The Formerly Utilized Sites Remedial Action Program (FUSRAP) is to evaluate the radiological conditions at former MED-US AEC sites. Purpose of the Ocean FUSRAP program is to assess the feasibility of ocean disposal of FUSRAP waste which contains trace natural radioactive materials. This paper presents soil characterization information on the Middlesex, NJ, Sampling Plant site, and reports preliminary ocean dispersion calculations. (DLC)

1982-01-01

203

Implementation of Raman lidar for profiling of atmospheric water vapor and aerosols at the SGP CART site

Site Error There's a problem with your browser or settings. ...

204

Short communication.

1995-05-01

205

ISR site

European web sites to watch the European Organization for Nuclear Research, CERN

A last look at the green field, in the triangular wedge of land stray radiation near the CERN accelerators.

1965-01-01

206

CERN Multimedia

Review of CERN's homepage/website (1 page).

2002-01-01

207

Electronic measures of surgical site infection: implications for estimating risks and costs.

Effects of Treatment According to Tattoo Color, Site and Duration with the Q-Switched Alexandrite Laser

PMID:21768762

2011-08-01

208

ENHANCED CONTROL OF MERCURY BY WET FLUE GAS DESULFURIZATION SYSTEMS - SITE 1 RESULTS

Full Text Available

209

Development of a site-specific following accident dose assessment system

No abstract prepared.

1999-05-01

210

Creating communities in cyberspace: pro-anorexia web sites and social capital.

The objectives of this project to interface the site-specific real-time radiological dose assessment system FADAS(Following Accident Dose Assessment System) to CARE. In this study, the results of the field tracer experiments conducted on the Younggwang site have been analysed. And the experimental procedure on Ulchin site has been introduced. The environmental characteristics on Ulchin and Wolsung has been investigated.

1997-12-15

211

Conduct of Operations Applicability Matrix for CMELS Low Hazard Facilities at LLNL Site 200

PMID:18387153

2008-05-01

212

Conduct of Operations Applicability Matrix for CMELS Light Science and Industry and General Industry Facilities at LLNL Site 200

No abstract prepared.

2008-04-22

213

Characterization of polluted sites. Assessment of pollutant behaviour and transfer in mediums; Caracterisation des sites pollues. L`evaluation du comportement et du transfert des polluants dans les milieux

No abstract prepared.

2008-04-22

214

Analysis of Site Amplification of Seismic Stations using Odesan Earthquake

After a presentation of methods and tools for the basic and extensive characterization of polluted sites and the study of evolution and transfer of current organic pollutants in the ground, the example of the rehabilitation of an old Rhone-Poulenc site (at Chauny, France) polluted with metals and arsenic, is described: soil and aquifer diagnosis, risk analysis and determination of migration schemes

1996-12-31

215

Site occupancies in ternary C15 ordered Laves phases

Full Text Available

2009-02-01

216

Predicting soil erodibility in northern Iraq using natural runoff plot data

Site occupancies in three C15-structured AB{sub 2}(X) Laves phases have been determined by Atom Location by CHanneling Enhanced MIcroanalysis (ALCHEMI). In NbCr{sub 2}(V), the results were consistent with exclusive site occupancies of Nb for the A sublattice and Cr and V for the B sublattice. The B-site occupancy of V is not expected from atom size effects alone. In NbCr{sub 2}(Ti), the results were consistent with Ti partitioning mostly to the A sites with some anti-site defects likely. In HfV{sub 2}(Nb), the results were consistent with Nb partitioning between the A and B sites. The results of the ALCHEMI analyses of these ternary C15 Laves phase materials will be discussed with respect to previously determined phase diagrams and first-principles total energy and electronic structure calculations.

1996-12-31

217

British Library Electronic Table of Contents (United Kingdom)

Data from natural runoff plots at two experimental sites in northern Iraq were used to predict soil erodibility in this semiarid region. The first site has a mean seasonal rainfall of about 400mm with the plots situated on 40% uniform slope; the soil at the site has a loam texture and belongs to the Xerorthent suborder; it has 46% of gravel by weight in its surface layer. The second site has a mean seasonal rainfall of more than 900mm with the plot situated on 17% uniform slope; the soil at the site is silty clay and belongs to the Calcixeroll suborder. At both sites, soil loss and runoff measurements were collected for two rainfall seasons. Results from both experimental sites showed that storm-to-storm variation in soil erodibility is high reflecting the importance of long term measureme...

2007-01-01

218

Salt repository project closeout status report

Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Durango, Colorado: Attachment 2, Geology report. Revised final report

This report provides an overview of the scope and status of the US Department of Energy (DOE`s) Salt Repository Project (SRP) at the time when the project was terminated by the Nuclear Waste Policy Amendments Act of 1987. The report reviews the 10-year program of siting a geologic repository for high-level nuclear waste in rock salt formations. Its purpose is to aid persons interested in the information developed during the course of this effort. Each area is briefly described and the major items of information are noted. This report, the three salt Environmental Assessments, and the Site Characterization Plan are the suggested starting points for any search of the literature and information developed by the program participants. Prior to termination, DOE was preparing to characterize three candidate sites for the first mined geologic repository for the permanent disposal of high-level nuclear waste. The ...

1988-06-01

219

Environmental data for sites in the National Solar Data Network

Detailed investigations of geologic, geomorphic, and seismic conditions at the Bodo Canyon disposal site were conducted. The purpose of these investigations was basic site characterization and identification of potential geologic hazards that could affect long-term site stability. Subsequent engineering studies, such as analyses of hydrologic and liquefaction hazards, used the data developed in these studies. The geomorphic analysis was employed in the design of effective erosion protection. Studies of the regional and local seismotectonic setting, which included a detailed search for possible capable faults within a 65 kilometer radius of the site, provided the basis for seismic design parameters. The scope of work performed included the following: Compilation and analysis of previous published and unpublished geologic literature and maps; Review of historical and instrumental earthquake data; Review ...

1991-12-01

220

Demonstration of innovative monitoring technologies at the Savannah River Integrated Demonstration Site

The environmental data for the NSDN are presented in the form of tables for each solar site. The solar sites are grouped into 12 zones, each of which consists of several adjacent states. The solar energy sites are in alphabetical sequence within each zone. The tables provide available meteorological data for reporting sites in the NSDN as follows: Insolation - the insolation table presents the total, diffuse, direct, maximum, and extra-terrestrial radiation for the solar site. It also shows the ratio of total extra-terrestrial radiation, as a percent. Temperature - the temperature table gives the average, daytime, nightime, maximum, minimum and inlet-water temperatures for the solar site. Additional tables are presented for some of these NSDN sites, supplying either wind or relative humidity data, or both. Wind - all of the passive and some ...

1980-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

221

[Molecular analysis of cytoplasmic male sterility]. Progress report, June 1, 1989--June 15, 1991

The Department of Energy`s Office of Technology Development initiated an Integrated Demonstration Program at the Savannah River Site in 1989. The objective of this program is to develop, demonstrate, and evaluate innovative technologies that can improve present-day environmental restoration methods. The Integrated Demonstration Program at SRS is entitled ``Cleanup of Organics in Soils and Groundwater at Non-Arid Sites.`` New technologies in the areas of drilling, characterization, monitoring, and remediation are being demonstrated and evaluated for their technical performance and cost effectiveness in comparison with baseline technologies. Present site characterization and monitoring methods are costly, time-consuming, overly invasive, and often imprecise. Better technologies are required to accurately describe the subsurface geophysical and geochemical features of a site and the nature and extent of ...

1993-12-31

222

Tungsten Effects on Phosphate-Dependent Biochemical Pathways are Species and Liver Cell Line Dependent

We have determined the actual RNA sequence of four mitochondrial genes: pcf, nad3, rps12, and coxII. Because plant mitochondrial proteins cannot be predicted from DNA sequences, the actual primary structures of the encoded proteins were unknown. We have gained information concerning the process of RNA editing. Editing can occur before splicing. Our data is consistent with the hypothesis that RNA editing is not simultaneous with transcription. Unlike other systems, the process of editing in plant does not exhibit a discernable direction. As a result of our RNA editing studies, we have produced a number of clones of the proper coding regions tube used for incorporating these mitochondrial genes into the nucleus. 6 refs., 4 figs.

1991-12-31

223

British Library Electronic Table of Contents (United Kingdom)

Tungsten, in the form of tungstate, polymerizes with phosphate, and as extensive polymerization occurs, cellular phosphorylation and dephosphorylation reactions may be disrupted, resulting in negative effects on cellular functions. A series of studies were conducted to evaluate the effect of tungsten on several phosphate-dependent intracellular functions, including energy cycling (ATP), regulation of enzyme activity (cytosolic protein tyrosine kinase [cytPTK] and tyrosine phosphatase), and intracellular secondary messengers (cyclic adenosine monophosphate [cAMP]). Rat noncancerous hepatocyte (Clone-9), rat cancerous hepatocyte (H4IIE), and human cancerous hepatocyte (HepG2) cells were exposed to 1-1000 mg/l tungsten (in the form of sodium tungstate) for 24 h, lysed, and analyzed for the ab...

2010-01-01

224

Targeted integration of baboon endogenous virus in the BEVI locus on human chromosome 6.

Strategies to optimize the outcome of children given T-cell depleted HLA-haploidentical hematopoietic stem cell transplantation

The infection of cultured human cells with baboon endogenous virus (BEV) frequently leads to an association of viral DNA with a specific genetic locus (termed BEVI, for baboon endogenous virus infection) on chromosome 6. Restriction endonuclease digestion of DNA from BEV-infected human cells and their derived somatic cell clones frequently revealed a common cellular DNA sequence in the proximity of one of the junctions between cellular DNA and the integrated virus. We propose that a short cellular DNA sequence, repeated on chromosome 6 and separated by unique DNA sequences, presents a high-affinity target for the integration of BEV in human cells. PMID:6401843

1983-01-13

225

British Library Electronic Table of Contents (United Kingdom)

The most advanced frontier of allogeneic hematopoietic stem cell transplantation (allo-HSCT) is represented by the use of an HLA-partially matched relative as donor. In this type of transplantation, donor-derived natural killer (NK) cells, which are alloreactive towardtoward recipient cells, significantly contribute to the eradication of leukemia blasts. Alloreactive NK cells may also kill host dendritic cells and T lymphocytes, thus preventing graft-versus-host disease and graft rejection, respectively. Sophisticated strategies of adoptive infusion of T-cell lines/clones specific for the most life-threatening pathogens (namely cytomegalovirus, Epstein-Barr virus, Aspergillus and Adenovirus) have been envisaged, and successfully tested in a few pilot trials, to protect the recipient in the...

2011-01-01

226

Searching for immunomodulatory sequences in sea bass (Dicentrarchus labrax L.): Transcripts analysis from thymus

British Library Electronic Table of Contents (United Kingdom)

The thymus is a key organ of the immune system in most vertebrates and, for this reason, it has been used in this paper for the generation of a normalized cDNA library from sea bass (Dicentrarchus labrax), one of the most extensively cultured species in South Mediterranean aquaculture. A total of 1632 ESTs from this library were initially analysed for sequence quality and vector sequences and, after this control, 1264 (77% of total clones sequenced) high-quality ESTs were further processed. The total collection of D. labrax thymus ESTs has been deposited in the EBI-GenBank-DBJ database (GenBank accession numbers from FN565576 to FN566839). The functional classification of ESTs was performed by Gene Ontology and KEGG annotation and, successively, the sequences were analysed using the Immuno...

2010-01-01

227

Purpurin is a key molecule for cell differentiation during the early development of zebrafish retina

British Library Electronic Table of Contents (United Kingdom)

Recently, we cloned purpurin cDNA as an upregulated gene in the axotomized fish retina. The retina-specific protein was secreted from photoreceptors to ganglion cell layer during an early stage of optic nerve regeneration in zebrafish retina. The purpurin worked as a trigger molecule for axonal regrowth in adult injured fish retina. During zebrafish development, purpurin mRNA first appeared in ventral retina at 2 days post-fertilization (dpf) and spread out to the outer nuclear layer at 3 dpf. Here, we investigated the role of purpurin for zebrafish retinal development using morpholino gene knockdown technique. Injection of purpurin morpholino into the 1-2 cell stage of embryos significantly inhibited the transcriptional and translational expression of purpurin at 3 dpf. In the purpurin mo...

2009-01-01

228

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase

British Library Electronic Table of Contents (United Kingdom)

We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step PCR pathogen detection using established real-time detection methods. We demonstrate that this Taq polymerase mutant (Taq M1) has similar PCR sensitivity and nuclease activity as the respective Taq wild-type DNA polymerase. In addition, and in marked contrast to the wild-type, Taq M1 exhibits a significantly increased reverse transcriptase activity especially at high temperatures (>60degreeC). RNA generally hosts highly stable secondary structure motifs, such as hairpins and G-quadruplexes, which complicate, or in the worst case obviate, reverse transcription (RT). Thus, RT at hi...

2010-01-01

229

Leishmania major ascorbate peroxidase overexpression protects cells against reactive oxygen species-mediated cardiolipin oxidation

British Library Electronic Table of Contents (United Kingdom)

Heme peroxidases are a class of multifunctional redox-active proteins found in all organisms. We recently cloned, expressed, and characterized an ascorbate peroxidase from Leishmania major (LmAPX) that was capable of detoxifying hydrogen peroxide. Localization studies using green fluorescent protein fusions revealed that LmAPX was localized within the mitochondria by its N-terminal signal sequence. Subcellular fractionation analysis of the cell homogenate by the Percoll density-gradient method and subsequent Western blot analysis with anti-LmAPX antibody further confirmed the mitochondrial localization of mature LmAPX. Submitochondrial fractionation analysis showed that the mature enzyme (?3.6?kDa shorter than the theoretical value of the whole gene) was present in the intermembrane space ...

2008-01-01

230

Isolation and characterization of an ornithine aminotransferase-related sequence (OATL3) mapping to 10q26

Identification of a functional nuclear export signal in the green fluorescent protein asFP499

The authors used a near full-length human ornithine [delta]-aminotransferase cDNA, huOAT6, as a probe under low stringency hybridization conditions to identify a new autosomal ornithine [delta]-aminotransferase-related sequence (OATL3). Cloning and characterization of this sequence reveal it to be a partial nonprocessed pseudogene corresponding to exon 3 and flanking intronic sequences of the ornithine [delta]-aminotransferase structural gene. Using somatic cell hybrids and fluorescence in situ hybridization, they mapped OATL3 to 10q26, adjacent to the ornithine [delta]-aminotransferase structural gene locus. 13 refs., 3 figs.

1993-08-01

231

Human and rat mast cell high-affinity immunoglobulin E receptors: Characterization of putative. alpha. -chain gene products

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

232

Genetic and environmental interactions determine plant defences against herbivores

The authors have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative {alpha} subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs encode an NH{sub 2}-terminal signal peptide, two immunoglobulin-like extracellular domains (encoded by discrete exons), a hydrophobic transmembrane region, and a positively charged cytoplasmic tail. The human and rat {alpha} subunits share an overall homology with one another and the immunoglobulin gene family, suggesting that they arose from a common ancestral gene and continue to share structural homology with their ligands. In addition, the rat gene is transcribed into at least three distinct forms, each of which yields a somewhat different coding sequence.

1988-03-01

233

British Library Electronic Table of Contents (United Kingdom)

Summary 1. Plants express multiple defensive traits, but little is known about the genetic stability and phenotypic plasticity of these traits in nature. To investigate sources of variation and their potential ecological consequences for herbivores, we combined field observations of cyanogenic lima bean with laboratory experiments. 2. Field studies in South Mexico revealed a distinct variability of cyanogenic traits within and among wild lima bean populations. To differentiate among genetic variation and the impact of ambient conditions on plant phenotypes, we used seed grown plants as well as clones propagated from high (HC) and low cyanogenic (LC) wild type plants. 3. In growth chamber experiments, we cultivated plants under three intensities each of drought and salt stress, nutrient sup...

2011-01-01

234

Food-grade gene expression in lactic acid bacteria

British Library Electronic Table of Contents (United Kingdom)

Abstract In the 1990s, significant efforts were invested in the research and development of food-grade expression systems in lactic acid bacteria (LAB). At this time, Lactococcus lactis in particular was demonstrated to be an ideal cell factory for the food-grade production of recombinant proteins. Steady progress has since been made in research on LAB, including Lactococcus, Lactobacillus and Streptococcus, in the areas of recombinant enzyme production, industrial food fermentation, and gene and metabolic pathway regulation. Over the past decade, this work has also led to new approaches on chromosomal integration vectors and host/vector systems. These newly constructed food-grade gene expression systems were designed with specific attention to self-cloning strategies, food-grade selection...

2011-01-01

235

Cytogenetic polyclonality of breast carcinomas: Association with Clinico-Pathological Characteristics and Outcome

British Library Electronic Table of Contents (United Kingdom)

Abstract Routinely used prognostic factors fail to predict clinical outcome in a significant proportion of breast cancer patients, implying that they can not detect some important biological characteristics. Chromosomal changes have been described in breast carcinomas for many years but their significance is not clear. We compared chromosomal changes with clinico-pathological characteristics and clinical outcome in 203 breast cancer patients with a follow-up of 9-18 years. Combining data from classical cytogenetics and flow cytometry revealed chromosomal abnormalities in 142 cases (70%). Of these, 51 (35.9%) contained two or more cytogenetically abnormal clones. Polyclonality was significantly associated with poor breast-cancer-specific survival (P = 0.03) within 5 years, independent of tu...

2011-01-01

236

Comparative study on the technological properties of latex and natural rubber from Hancornia speciosa Gomes and Hevea brasiliensis

British Library Electronic Table of Contents (United Kingdom)

This work reports a systematic comparative study of the technological properties of natural lattices and rubbers extracted from Hancornia speciosa Gomes and Hevea brasiliensis [(Willd. ex Adr. de Juss.) Muell.-Arg.] (clone RRIM 600) trees from 11 collections in Brazil throughout 2004. Natural rubber latex particle sizes and distributions were quite similar with an average diameter around 1 mm. Molecular weight, Wallace plasticity, and Mooney viscosity values were approximately the same for both rubbers. Fourier transform infrared spectroscopy peaks characteristic of natural rubber were observed for both Hancornia and Hevea. The measured differences in technological properties included lower values for Hancornia dry rubber content, % ash, % nitrogen, and plasticity retention index but highe...

2009-01-01

237

Chromosomal localization and structure of the human type II IMP dehydrogenase gene

Characteristics of radiation-induced neoplastic transformation in vitro

We determined the chromosomal localization and structure of the gene encoding human type II inosine 5{prime}-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.1{yields}p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH were isolated and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.

1994-05-01

238

Bioinformatic analysis of BBTV satellite DNA in Hainan

Data are presented to support the hypothesis that the initial step in the morphologic transformation of irradiated rodent (BALB/3T3) cells is a frequent cellular event involving a large fraction of the irradiated population. This process appears to involve DNA damage, but not to represent a targeted mutation in specific structural gene(s). Morphologic transformation and immortalization appear to be distinct steps in the overall process of transformation. In contradistinction to rodent cells, immortalization is a very rare event in human diploid cells which is induced at extremely low frequencies. The hypothesis is presented that immortality develops among clones of cells bearing stable chromosomal rearrangements which emerge during the proliferation of a population of radiation damaged cells.

1986-01-01

239

British Library Electronic Table of Contents (United Kingdom)

Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiatio...

2011-01-01

240

Antioxidant phytochemicals and gingerol content in diploid and tetraploid clones of ginger (Zingiber officinale Roscoe)

British Library Electronic Table of Contents (United Kingdom)

Fresh rhizomes of 18 diploid and tetraploid genotypes of Indian ginger, including commercial cultivars and experimental genotypes, were assayed for gingerol content and antioxidant activity. The tetraploid ginger type was derived from the respective diploid ginger by shoot tip culture. [6]-Gingerol was identified as the major pungent phenolic compound in all samples (mean 1354.78+/-470mg/g), while [8]- (mean 180.94+/-45mg/g) and [10]-gingerol (mean 234+/-79mg/g) occurred in lower concentrations. The total gingerol content of the tetraploid type was much higher than that of the respective diploid type and especially the [10]-gingerol. Both diploid and tetraploid types of the cv. Meghalaya Local contained the highest level of gingerol. At both ploidy levels, the genotypes showed significant ...

2010-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

ALK, the Key Gene for Gelatinization Temperature, is a Modifier Gene for Gel Consistency in Rice

British Library Electronic Table of Contents (United Kingdom)

Abstract Gelatinization temperature (GT) is an important parameter in evaluating the cooking and eating quality of rice. Indeed, the phenotype, biochemistry and inheritance of GT have been widely studied in recent times. Previous map-based cloning revealed that GT was controlled by ALK gene, which encodes a putative soluble starch synthase II-3. Complementation vector and RNAi vector were constructed and transformed into Nipponbare mediated by Agrobacterium. Phenotypic and molecular analyses of transgenic lines provided direct evidence for ALK as a key gene for GT. Meanwhile, amylose content, gel consistency and pasting properties were also affected in transgenic lines. Two of four nonsynonymous single nucleotide polymorphisms in coding sequence of ALK were identified as essential for GT. ...

2011-01-01

242

Health assessment for Davis GSR Landfill, Glocester, Rhode Island, Region 1. CERCLIS No. RID980731459. Preliminary report

Do Thinning and Burning Sites Revegetated after Bauxite Mining Improve Habitat for Terrestrial Vertebrates?

The Davis GSR Landfill (GSR) is listed by the U.S. Environmental Protection Agency on the National Priorities List. Preliminary on-site sampling results have demonstrated volatile organic compounds in ground water and surface water. The contaminants present in groundwater at this site are trichloroethylene, ethylbenzene, toluene, chloromethane, tetrahydrofuran, 1,1,1-trichloroethane, and 1,2 dichloroethane. GSR represents a potential public health concern to area residents. However, information available on this site is not currently adequate to determine if a public health concern exists to these residents. At a minimum, future investigations of this site should include a characterization of the site and site contaminants, and a characterization of the hydrogeology of the area.

1989-04-10

243

British Library Electronic Table of Contents (United Kingdom)

Thinning and burning forests established on revegetated mine pits in jarrah (Eucalyptus marginata) forests of south-west Australia is being considered as a management option to accelerate succession in sites with excessive tree densities. To assess the impact of thinning and burning on reptiles and small mammals, we installed trapping grids in eight thinned and burned sites, each paired with untreated controls. Of the eight pairs, four were in rehabilitated sites (planted with nonlocal species) and four were in restored sites (seeded with local species). Thinning and burning had no significant impact on the small mammal community, although Cercatetus concinnus was more abundant in rehabilitated sites. In contrast, thinning and burning significantly increased reptile abundance and species r...

2010-01-01

244

Current status of siting a new near surface repository in Romania

Visual and refractive outcome of one-site phacotrabeculectomy compared with temporal approach phacoemulsification

Full text: The site selection process for a near surface repository dedicated for the radioactive waste resulted from the Cernavoda NPP operation and decommissioning started early in 90's. Each site selection stage included the collection of data from specific field and laboratory works as well as the appropriate safety performance evaluation. In order to assess/confirm the performance of the natural barrier of the Saligny site, the radionuclide concentration in the disposal system compartments has been evaluated, as complementary safety indicator of repository. The siting process was made in accordance with national and international regulations and standards and using a conceptual design similar to those used at L'Aube (France), ElCabril (Spain) or Mohovce (Slovak Republic). ANDRAD, the Romanian waste management organization that has continued the siting process in the last three ...

2009-05-27

245

Uptake of injected 125I-ricin by rat liver in vivo. Subcellular distribution and characterization of the internalized ligand.

BackgroundWe aimed to compare visual and refractive outcome following phacoemulsification and intraocular lens implant (IOL) and combined one-site phacotrabeculectomy.MethodWe...Full Text Available

2008-09-01

246

Thermodynamic and kinetic aspects of surface acidity

Subcellular-fractionation techniques were used to characterize the endocytic pathway followed by ricin in rat liver in vivo and tentatively identify the site(s) at which the ricin interchain disulphide...Full Text Available

1992-05-15

247

The autoradiographic localization of paraquat in the lung

Our research in the general area of acid catalysis involves the characterization of solid acidity and the corresponding assessment of catalytic performance of acidic materials. Acid characterization studies are required to provide essential information about the type of acid site (i.e., Lewis versus Bronsted), the strength of the sites, and the mobility of molecules adsorbed on the acid sites. An accurate measure of acid strength is given by the heat of adsorption of a basic probe molecule on the acid site. A thermodynamic representation of the mobility of adsorbed species on these sites is given by the entropy of adsorption. Important techniques used in these acid site characterization studies include microcalorimetry, thermogravimetric measurements, temperature programmed desorption, infrared spectroscopy and solid state nuclear magnetic resonance. The ...

1992-01-01

248

Study on quality assurance for high-level radioactive waste disposal project

Paraquat poisoning in mammals results in a characteristic lung lesion manifested principally as progressive pulmonary fibrosis. Paraquat is actively concentrated into the lung but the site of uptake remains undefined. A method is described for the autoradiographic localization of paraquat in rats. Preliminary evidence for the site of uptake implicates the bronchiol. (author).

249

Sorption phenomena of methanol on heat treated coal; Netsushori wo hodokoshita sekitan no methanol kyuchaku tokusei

The U.S. Department of Energy (DOE) has developed comparatively detailed quality assurance requirements for the high-level radioactive waste disposal systems. Quality assurance is recognized as a key issue for confidence building and smooth implementation of the HLW program in Japan, and Japan is at an initial phase of repository development. Then the quality assurance requirements at site research and site selection, site characterization, and site suitability analysis used in the Yucca Mountain project were examined in detail and comprehensive descriptions were developed using flow charts. Additionally, the applicability to the Japan high-level radioactive waste disposal project was studied. The examination and study were performed for the following QA requirements: The requirements that have the relative importance at site research and site selection, ...

2005-03-01

250

Secondary structure formation and DNA instability at fragile site FRA16B

Experiments were carried out to learn methanol sorption characteristics of heat-treated coal. When Taiheiyo coal is heat-treated at 125{degree}C, performed with a first methanol adsorption at 25{degree}C, and then desorption at 25{degree}C, a site with strong interaction with methanol and a site with relatively weak interaction are generated in test samples. A small amount of methanol remains in both sites. Then, when the methanol is desorbed at as low temperature as 70{degree}C, the methanol in the site with strong interaction remains as it has existed therein, but the methanol in the site with relatively weak interaction desorbs partially, hence the adsorption amount in a second adsorption at 25{degree}C increases. However, when desorption is performed at as high temperature as 125{degree}C, the methanol in the site with strong interaction also desorbs, ...

1996-10-28

251

Oak Ridge National Laboratory Technology Logic Diagram. Volume 2, Technology Logic Diagram: Part C, Waste Management

Human chromosomal fragile sites are specific loci that are especially susceptible to DNA breakage following conditions of partial replication stress. They often are found in genes involved in tumorigenesis...Full Text Available

2010-05-01

252

Multiple integration sites for Moloney murine leukemia virus in productively infected mouse fibroblasts.

This report documents site remediation at ORNL, including ORNL site characterization technologies, waste management and robotics and automation of the laboratory for waste processing and analysis.

1993-09-01

253

Impaired replication dynamics at the FRA3B common fragile site

The integration sites for viral DNA in cells infected with Moloney murine leukemia virus (M-MuLV) were studied by restriction endonuclease cleavage of cellular DNA followed by electrophoresis in agarose...Full Text Available

1979-06-01

254

COST ESTIMATING TOOLS AND RESOURCES FOR ADDRESSING SITES UNDER THE BROWNFIELDS INITIATIVE

Chromosomal common fragile sites (CFSs) are genetically unstable regions of the genome that are induced by conditions that impair DNA replication. In this report, we show that treatment with the DNA...Full Text Available

2010-01-01

255

British waste plans face fierce local opposition

Brownfields redevelopment contributes to the revitalization of communities across the U.S. Reuse of these abandoned, contaminated sites spurs economic growth, builds community pride, protects public health, and helps maintain our nation's "greenfields," often at a relatively low ...

256

Being a binding site: Characterizing residue composition of binding sites on proteins

UK Nirex has announced four sites which it wishes to assess for the disposal of low-level and short-lived intermediate-level radioactive wastes. There is local opposition at all four of the potential sites. (U.K.).

1986-05-01

257

Albumin binding of photobilirubin II.

The Protein Data Bank contains the description of more than 45,000 three-dimensional protein and nucleic-acid structures today. Started to exist as the computer-readable depository of crystallographic...Full Text Available

258

A global optimization algorithm for protein surface alignment

Photobilirubin II, a stereoisomer of bilirubin, binds to human serum albumin at a single binding site (K = 2.2 x 10(6)M-1), presumably the high-affinity bilirubin-binding site. Binding in the secondary...Full Text Available

1983-07-01

259

Olfactomedin 4 suppresses prostate cancer cell growth and metastasis via negative interaction with cathepsin D and SDF-1.

BackgroundA relevant problem in drug design is the comparison and recognition of protein binding sites. Binding sites recognition is generally based on geometry often combined with...Full Text Available

260

Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase

The human olfactomedin 4 gene (OLFM4) encodes an olfactomedin-related glycoprotein. OLFM4 is normally expressed in a limited number of tissues, including the prostate, but its biological functions in prostate are largely unknown. In this study, we found that OLFM4 messenger RNA was reduced or undetectable in prostate cancer tissues and prostate cancer cell lines. To study the effects of OLFM4 on prostate cancer progression, we transfected PC-3 prostate cancer cells with OLFM4 to establish OLFM4-expressing PC-3 cell clones. The OLFM4-expressing PC-3 cell clones were found to have decreased proliferation and invasiveness compared with vector-transfected control PC-3 cells in vitro. In addition, nude mice injected with OLFM4-expressing PC-3 cells demonstrated reduced tumor growth and bone invasion and metastasis compared with mice injected with vector-transfected control cells. Mechanistic studies revealed that OLFM4 may exhibit its anticancer ...

2011-04-05

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

Cloning, Expression, Crystallization and Preliminary Crystallographic Analysis of a Pentapeptide-repeat Protein (Rfr23) from the Bacterium Cyanothece 51142l

By catalyzing the rate-limiting step in adipose tissue lipolysis, hormone-sensitive lipase (HSL) is an important regulator of energy homeostasis. The role and importance of HSL in tissues other than adipose are poorly understood. We report here the cloning and expression of a testicular isoform, designated HSL{sub tes}. Due to an addition of amino acids at the NH{sub 2}-termini, rat and human HSL{sub tes} consist of 1068 and 1076 amino acids, respectively, compared to the 768 and 775 amino acids, respectively, of the adipocyte isoform (HSL{sub adi}). A novel exon of 1.2 kb, encoding the human testis-specific amino acids, was isolated and mapped to the HSL gene, 16 kb upstream of the exons encoding HSL{sub adi}. The transcribed mRNA of 3.9 kb was specifically expressed in testis. No significant similarity with other known proteins was found for the testis-specific sequence. The amino acid composition differs from the HSL{sub adi} sequence, with a notable hydrophilic ...

1996-08-01

262

Clofibrate-induced cytochrome P450-lauric acid omega hydroxylase(P450LA omega):purification, cDNA cloning, sequence and regulation

A unique feature of cyanobacteria genomes is the abundance of genes that code for hypothetical proteins containing tandem pentapeptide repeats approximately described by the consensus motif A(N/D)LXX. To date, the structures of two pentapeptide-repeat proteins (PRPs) have been determined, with the tandem pentapeptide-repeat sequences observed to adopt a novel type of right-handed quadrilateral ?-helix, or Rfr-fold, in both structures. One structure, Mycobacterium tuberculosis MfpA, is a 183-residue protein that contains 30 consecutive pentapeptide repeats and appears to offer antibiotic resistance by acting as a DNA mimic. The other structure, Cyanothece 51142 Rfr32, is a 167-residue protein that contains 21 consecutive pentapeptide repeats. The function of Rfr32, like the other 35 hypothetical PRPs identified in the genome of Cyanothece, is unknown. In an effort to understand the role of PRPs in cyanobacteria and to better characterize the structural properties of Rfr-folds with ...

2006-01-01

263

Vegetation re-establishment on a hardwood forest site denuded by brine

A cytochrome P450 that hydroxylates lauric acid at the 12 position (P450LA omega) was isolated from liver microsomes of clofibrate treated rats. P450LA omega was immunologically distinct from P450s a,b,c,d,e,f,g,h,j,PB1, and PCN1. Polyclonal antibody against P450LA omega was utilized to screen a gt11 cDNA library. A clone (pP450LA omega), was isolated and its sequence determined. The P450LA omega mRNA is a minimum 2387 nts in length and codes for a P450 of Mr.58,222 daltons. This protein shares less than 35% amino acid similarity with P450s b,c,d,e,f,PB1, and PCN1; however, it does contain a hydrophobic amino terminal peptide and a conserved sequence surrounding the Cys residue at position 456, which is similar to other microsomal P450s. P450LA omega is present at high levels in untreated rat kidney and is induced by clofibrate in both kidney and liver. This induction is the result of an accumulation of mRNA through a rapid transcriptional activation of the P450LA ...

1986-05-01

264

The in-situ gas migration test (GMT) at the Grimsel test site: re-saturation phase of silo type repository

Brine from active oil wells seeped through the soil of a forested site in north-western Pennsylvania and killed all vegetation in its path, leaving the affected area unproductive and unsightly. After the brine source was eliminated, herbaceous plants, shrubs and forest tree seedlings became established and developed rapidly. Establishment began in the first year and by Year 4 the site had developed into a healthy young forest. Results show that Allegheny hardwood forest sites damaged by brine water will rapidly revegetate once the brine water discharge is stopped if there is a seed supply and if the area is fenced to exclude deer. 3 figs., 12 refs.

1989-04-01

265

Surface Temperature - My NASA Data

No abstract prepared.

2002-07-01

266

Spatial Variability of Reactive Mineral and Radionuclide Kd Distributions in the Tuff Confining Unit: Yucca Flat, Nevada Test Site

Surface temperature is measured with an infrared thermometer. Measuring Surface Temperature. Nine sample points are selected with a site of uniform land ...

267

Site characterization progress report: Yucca Mountain, Nevada, October 1, 1990--March 31, 1991; Number 4

Abstract not provided

2008-03-03

268

SRIPP EIS April 21, 2009 Scoping Meeting Minutes

In accordance with the requirements of Section 113 (b) (3) of the Nuclear Waste Policy Act of 1982, as amended (NWPA), the US Department of Energy (DOE) has prepared this report on the progress of site characterization activities at Yucca Mountain, Nevada, for the period October 1, 1990, through March 31, 1991. This report is the fourth in a series of reports that are issued at intervals of approximately six months during site characterization. The report covers a number of initiatives to improve the effectiveness of the site characterization program, and covers continued efforts related to preparatory activities, Study Plans, and performance assessment.

1991-10-01

269

Robotics and Automation Activities at the Savannah River Site: A Site Report for SUBWOG 39F

clam and oyster aquaculture permit sites, clam and oyster aquaculture suitability models, and Phragmites coverage. ...

270

Roadmap to the SRS computing architecture

The Savannah River Site has successfully used robots, teleoperators, and remote video to reduce exposure to ionizing radiation, improve worker safety, and improve the quality of operations. Previous reports have described the use of mobile teleoperators in coping with a high level liquid waste spill, the removal of highly contaminated equipment, and the inspection of nuclear reactor vessels. This report will cover recent applications at the Savannah River, as well as systems which SRS has delivered to other DOE site customers.

1995-09-28

271

Mechanism of dehydroxylation of naturally occurring high-silica zeolites involving the formation of Lewis acid sites

This document outlines the major steps that must be taken by the Savannah River Site (SRS) to migrate the SRS information technology (IT) environment to the new architecture described in the Savannah River Site Computing Architecture. This document proposes an IT environment that is {open_quotes}...standards-based, data-driven, and workstation-oriented, with larger systems being utilized for the delivery of needed information to users in a client-server relationship.{close_quotes} Achieving this vision will require many substantial changes in the computing applications, systems, and supporting infrastructure at the site. This document consists of a set of roadmaps which provide explanations of the necessary changes for IT at the site and describes the milestones that must be completed to finish the migration.

1994-07-05

272

Indian Academy of Sciences (India)

Using low-temperature adsorbed dihydrogen and carbon monoxide as molecular probes, the dehydroxylation of the hydrogen forms of the zeolites Y, and ZSM-5 has been studied. The high stability of the high-silica zeolites to dealumination and their difference from faujasites has been established as being due not only to the strength of their Broensted acid sites but also to the nature of their Lewis acid sites. The chemical properties of the Lewis acid sites and their possible role in catalytic reactions are discussed.

1987-11-01

273

Logistics (contd.)

start within 6-12 months with small number of journals test and quality check with these journals already having full text sites ...

274

Interaction Of Human La Protein With The Internal Ribosome Entry Site Of Hepatitis C Virus : Functional Role In Mediating Internal Initiation Of Translation

Indian Institute of Science Theses & Dissertations (India)

Full Text Available

2004-07-01

275

Inland penetration of sea breeze around Wolsung NPP site

Inland penetration of sea breeze around Wolsung NPP site

A three dimensional sea-land breeze model has been employed for the study on the inland penetration of sea breeze around Wolsung NPP site. In this study, the sea breeze simulation was carried out under the weak northwesterly geostrophic wind (3.2 m/s, 339 .deg.) at 850 hPa in Spring. The results showed that sea breezes developed near Wolsung site penetrated into about 20 km inland under the weak northwesterly geostrophic wind in Spring. This result agreed with observation data around Wolsung site on May 1996.

1997-07-01

276

Hazardous waste operational plan for site 300

A three dimensional sea-land breeze model has been employed for the study on the inland penetration of sea breeze around Wolsung NPP site. In this study, the sea breeze simulation was carried out under the weak northwesterly geostrophic wind (3.2 m/s, 339 .deg.) at 850 hPa in Spring. The results showed that sea breezes developed near Wolsung site penetrated into about 20 km inland under the weak northwesterly geostrophic wind in Spring. This result agreed with observation data around Wolsung site on May 1996.

1997-11-06

277

Gases in insulating oil - analysis of dissolved gases using a portable chromatograph

This plan outlines the procedures and operations used at LLNL's Site 300 for the management of the hazardous waste generated. This waste consists primarily of depleted uranium (a by-product of U-235 enrichment), beryllium, small quantities of analytical chemicals, industrial type waste such as solvents, cleaning acids, photographic chemicals, etc., and explosives. This plan details the operations generating this waste, the proper handling of this material and the procedures used to treat or dispose of the hazardous waste. A considerable amount of information found in this plan was extracted from the Site 300 Safety and Operational Manual written by Site 300 Facility personnel and the Hazards Control Department.

1982-02-12

278

EPR power pattern analysis for cubic sites of Fe"3"+ in MgO

New technological advances make it possible to perform on site gas-in-oil analysis quickly and efficiently. Gas-in-oil analysis is crucial in assessing the status of oil-filled electrical equipment. The new technique is used for extracting and measuring gases dissolved in electrical insulating oil. On-site sampling requires the use of a specially designed 100 ml capacity syringe. Extraction of the dissolved gases is made with a precise ratio of oil to air. A comparison was made between the analysis of the on-site and the laboratory techniques. Results showed that the detection limits of the on-site technique were comparable to those obtained by laboratory methods.

1996-07-01

279

Cholera toxin binding sites in yeast triggers biochemical pathway

A complete electron paramagnetic resonance power pattern characterization of Fe"3"+ in cubic sites in presented. A one-to-one correspondence among the peaks appearing in the powder pattern and the outer fine-structure transitions (Mnot = 1/2 ) observed in the single crystal along the , , and directions is shown. It is shown that the process of mechanically grinding the single crystal to a powder (particle size approx.1--10 #mu#) does not remove the cubic symmetry sites. No axial or lower symmetry sites which may be induced by lattice distortion of the crystallites due to strain have been observed.

1984-01-01

280

1998 Annual report on waste generation and waste minimization progress as required by DOE order 5400.1 Hanford Site

... e Biologia Molecular (SBBq), Sao Paulo, SP (Brazil) 217 p. APPLIED LIFE

1998-05-23

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

281

No abstract prepared.

1999-02-25

282

165.37 22,_

Uranium Mill Tailings Remedial Action Project (UMTRAP), Slick Rock, Colorado, Revision 1, Volume 4. Calculations, Final design for construction

Aluminum electrolytic capacitors have a long history of reliable operation in unattended field sites. However, their use in the expected space applications ...

283

The implications of episodic nonequilibrium fracture-matrix flow on site suitability and total system performance

Volume four contains calculations for: Borrow areas--site evaluation; temporary facilities--material quantities; embankment quantities--excavation and cover materials; Burro Canyon site excavation quantities--rippable and unrippable materials; site restoration--earthwork quantities and seeding; and bid schedule quantities and material balance.

1995-09-01

284

The fatty acid analogue 11-(dansylamino)undecanoic acid is a fluorescent probe for the bilirubin-binding sites of albumin and not for the high-affinity fatty acid-binding sites.

We apply our work on fracture- and matrix-dominated flow to develop a conceptual model of hydrological flow processes in the unsaturated zone at Yucca Mountain. The possibility of fracture-dominated flow is discussed, and various deductions are made on its impact on natural and total system performance, site characterization activities, and site suitability determination.

1992-04-12

285

The (--)(/sup 3/H)dihydroalprenolol binding to rat adipocyte membranes: an explanation of curvilinear Scatchard plots and implications for quantitation of beta-adrenergic sites

1. The fluorescent fatty acid probe 11-(dansylamino)undecanoic acid (DAUDA) binds with high affinity to bovine and human serum albumin (BSA and HSA) at three sites. 2. The Kd of the primary binding...Full Text Available

1990-08-15

286

TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene.

In rat adipocyte membranes, both beta-adrenergic agonists and beta-adrenergic antagonists competed with (--)(/sup 3/H)dihydroalprenolol for high affinity (KD 2-4 nM) and low capacity binding sites. The antagonists but not the agonists competed with (--)(/sup 3/H)dihydroalprenolol for lower affinity and higher capacity sites. The present studies were performed in order to characterize the adipocyte beta-adrenergic receptor and distinguish it from low affinity, higher capacity sites which were heat-labile and not stereoselective. When isoproterenol was used to define the nonspecific binding, saturation studies showed a single binding site with a capacity of approximately 100 fmol/mg membrane protein (corresponding to approximately 50,000 sites/adipocyte). Binding was saturated by 10 nM (--)(/sup 3/H)dihydroalprenolol. Approximate KD's of 204 nM were observed. Kinetic analysis ...

1982-09-01

287

Structural basis of binding of fluorescent, site-specific dansylated amino acids to human serum albumin

We report the existence of a sixth replication arrest site, TerF, that is located within the coding sequences of the rcsC gene, a negative regulator of capsule biosynthesis. The TerF site is oriented...Full Text Available

1992-12-01

288

Remedial action plan and site design for stabilization of the inactive uranium mill tailings sites at Rifle, Colorado. Volume 5, Addenda D6--D8 to Appendix D: Final report

Human serum albumin (HSA) has two primary binding sites for drug molecules. These sites selectively bind different dansylated amino acid compounds, which—due to their intrinsic fluorescence—have...Full Text Available

2011-04-01

289

Interim remedial measures proposed plan for the 200-ZP-1 Operable Unit, Hanford Site, Washington

This volume contains appendices D6 through D8 containing laboratory test data: from MK-F investigation, 1987, Old Rifle and New Rifle sites; on bentonite amended radon barrier material; and from MK-F investigation, 1987, riprap tests.

1990-02-01

290

Evaluation of indoor and outdoor climate on sites polluted with volatile organic chemicals

The purpose of this interim remedial measures (IRM) proposed plan is to present and solicit public comments on the IRM planned for the 200-ZP-1 Operable Unit at the Hanford Site in Washington state. The 200-ZP-1 is one of two operable units that envelop the groundwater beneath the 200 West Area of the Hanford Site.

1993-12-01

291

Environmental data for sites in the national solar data network

Papers presented at a meeting on indoor and outdoor climates on sites polluted with volatile organic chemicals. The papers deal with the subject of evaporation of organic chemicals on the polluted sites in relation to the influence on indoor and outdoor climates. Themes dealt with are diffusion through soils and transport of pollutants from the soil into buildings. (AB).

1993-11-04

292

Charts estimate gas-turbine site performance

The Department of Energy's National Solar Data Program established solar energy systems in residential and commercial buildings across the United States. These solar sites are linked to Vitro Laboratories Division's computer in the National Solar Data Network (NSDN). Vitro collects and analyzes data from this network to determine the thermal performance of each of the solar systems. The network consists of: (1) sensors which measure key performance parameters at a selected site; (2) a Site Data Acquisition Subsystem (SDAS); (3) telephone transmission circuits; and (4) a Central Data Processing System (CDPS). For the majority of parameters, raw data is collected approximately every five minutes. Solar insolation and certain other parameters, which are subject to rapid variance, are sampled every 32 seconds. Environmental information collected at the sites for the reporting month are ...

1980-07-01

293

COST ESTIMATING TOOLS AND RESOURCES FOR ADDRESSING SITES UNDER THE BROWNFIELDS INITIATIVE (SYSTEMS ANALYSIS BRANCH, SUSTAINABLE TECHNOLOGY DIVISION, NRMRL)

Nomographs have been developed to simplify site performance estimates for various types of gas turbine engines used for industrial applications. The nomographs can provide valuable data for engineers to use for an initial appraisal of projects where gas turbines are to be considered. General guidelines for the selection of gas turbines are also discussed. In particular, site conditions that influence the performance of gas turbines are described.

1988-05-09

294

Breckinridge Project, initial effort. Report VII, Volume III. Cultural resource assessment socioeconomic background data

EPA's Brownfields Economic Redevelopment Initiative was established to assist states, site planners and other community stakeholders to work together to assess, cleanup and sustainably reuse brownfields sites. EPA has developed a series of guides to help these parties effectively...

295

Analysis of algorithms for ultra high speed relays for the protection of high voltage transmission lines

This report has been prepared in conjunction with an environmental baseline study for a commercial coal conversion facility being conducted by Ashland Synthetic Fuels, Inc. (ASFI) and Airco Energy Company (AECO). This report represents a cultural resource assessment for the proposed plant site and two potential solid waste disposal areas. This assessment presents data collected by Dames and Moore during a recent archaeological reconnaissance of the unsurveyed southeastern portion of the proposed plant site and two potential solid waste disposal areas. Also, results of two previous surveys on the northern and southwestern portion of the plant site for American Smelting and Refining Company (ASARCO) and Kentucky Utilities are included. The Dames and Moore survey of the southeastern portion of the plant site identified one archaeological site, three standing structures and one historic ...

296

Surveillance and maintenance activities of waste area groupings at Oak Ridge National Laboratory. Annual summary report for period ending September 30, 1991: Environmental Restoration Program

The object of this research is the evaluation of the performance of ultra high speed relays (UHSR's) used for protection of a-c transmission lines. For purposes of this report, these are relays whose response time is less than a quarter of a cycle of the 60 Hz wave (i.e. 4.167 ms.). To identify relaying schemes that may comply with this definition, a literature survey was undertaken. The selected relays were studied in detail and modeled on a digital computer. A theoretical description of these relays is presented. Records of real transient data as well as of simulated data were used as input to the digital models of relays. The real data were recorded by means of monitoring stations connected to the Florida Power and Light Company transmission lines. The simulated data were obtained by modeling the relevant parts of the utility's transmission system using a University of British Columbia simplified version of the well known ...

1984-01-01

297

Surveillance and maintenance activities of waste area groupings at Oak Ridge National Laboratory

Surveillance and maintenance (S & M) of 75 sites were conductd by the Remedial Action Section for the Environmental Restoration Program for surplus facilities and sites contaminated with radioactive materials and/or hazardous chemicals. S & M activities on these sites were conducted from the end of their operating life until final facility disposal or site stabilization. The objectives of the Waste Area Grouping S & M Program are met by maintaining a program of routine S & M as well as by implementing interim corrective maintenance when deemed necessary as a result of site surveillance. This report briefly presents this program`s activities and includes tables indicating tank levels and dry well data for FY 1991.

1991-12-01

298

Restauration of the Clinton site; Restauration du site Clinton

Surveillance and maintenance (S M) of 75 sites were conductd by the Remedial Action Section for the Environmental Restoration Program for surplus facilities and sites contaminated with radioactive materials and/or hazardous chemicals. S M activities on these sites were conducted from the end of their operating life until final facility disposal or site stabilization. The objectives of the Waste Area Grouping S M Program are met by maintaining a program of routine S M as well as by implementing interim corrective maintenance when deemed necessary as a result of site surveillance. This report briefly presents this program's activities and includes tables indicating tank levels and dry well data for FY 1991.

1991-12-01

299

Remedial investigation/feasibility study for the David Witherspoon, Inc., 901 Site, Knoxville, Tennessee: Volume 1

Mining of copper and zinc took place at the Clinton Mine located in the Estrie region of Quebec between 1973 and 1975, during which time 115,000 tons of mineral were treated at the Solbec plant. In this poster presentation, the authors provided the background information pertaining to this mine and described the state in which it was in 1994. The pit was covered with good quality water, with a pH of 7,1 and copper and zinc content ranging between 0,1 and 0,6 mg/l, and iron ranging between 0,2 and 1,8 mg/l. The tailings basin drainage was found to have a pH of between 3,6 and 6,1. A program for the characterization of the site was implemented and the concept for the restauration of the Clinton site was developed and implemented. The work carried out at the site is discussed during this presentation as well as the monitoring taking place. 27 figs.

2000-07-01

300

An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes

This remedial investigation (RI)/feasibility study (FS) supports the selection of remedial actions for the David Witherspoon, Inc. 901 Maryville Pike Site in Knoxville, Tennessee. Operations at the site, used as a recycling center, have resulted in past, present, and potential future releases of hazardous substances in to the environment. This Site is a Tennessee Superfund site. A phased approach was planned to (1) gather existing data from previous investigations managed by the Tenn. Dept. of Environment and Conservation; (2) perform a preliminary RI, including risk assessments, and an FS with existing data to identify areas where remedial action may be necessary; (3) gather additional field data to adequately define the nature and extent of risk-based contaminants that present identifiable threats to human and/or ecological receptors; and (4) develop remedial action alternatives to reduce risks to ...

1996-10-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

Active catalytic sites in the ammoxidation of propane and propene over V-Sb-O catalysts

The authors have identified a protein present only in erythroid cells that binds to two adjacent sites within an enhancer region of the chicken {beta}-globin locus. Mutation of the sites, so that binding by the factor can no longer be detected in vitro, leads to a loss of enhancing ability, assayed by transient expression in primary erythrocytes. Binding sites for the erythroid-specific factor (Eryf1) are found within regulatory regions for all chicken globin genes. A strong Eryf1 binding site is also present within the enhancer of at least one human globin gene, and proteins from human erythroid cells (but not HeLa cells) bind to both the chicken and the human sites.

1988-08-01

302

A study of binary mixture boiling: boiling site density and subcooled heat transfer

The ammoxidation of propane over VSb{sub y}O{sub x} catalysts (y=1, 2, 5) was investigated with respect to the role of different oxygen species in the selective and non selective reaction steps using transient experiments in the Temporal Analysis of Products (TAP) reactor. Only lattice oxygen is involved in the oxidation reactions. Using isotopic labelled oxygen it is shown that two different active sites exist on the surface. On site A, which can be reoxidized faster by gas phase oxygen compared to site B, mainly CO is formed. On site B CO{sub 2} and acrolein as well as NO and N{sub 2}O in the presence of ammonia in the feed gas are formed and reoxidation mainly occurs with bulk lattice oxygen. (orig.)

1998-12-31

303

A quantum theoretical study of reactions of methyldiazonium ion with DNA base pairs

(Boiling site densities and heat-transfer coefficients have been measured for ethanol-water and ethanol-benzene mixtures at 1.01 bar for a heated vertical brass disk. A strong effect of composition on the boiling site density was observed, which was attributed to the nature of the activation of the boiling surface and mass diffusion effects. The boiling heat-transfer coefficient was found to decrease with increasin subcooling, but for the mixtures at a given level of subcooling the decrease was less than that for the single components and azeotropic mixtures.) The heat-transfer coefficient at a given heat flux was seen to be quite insensitive to the very large increase in boiling site density in comparing the pure water and the ethanol-water azeotrope results, leading one to question pool boiling models that predict heat-transfer rates on the basis of boiling site density.

1985-05-01

304

British Library Electronic Table of Contents (United Kingdom)

Methylation of the DNA bases in the Watson-Crick GC and AT base pairs by the methyldiazonium ion was investigated employing density functional and second order Moller-Plesset (MP2) perturbation theories. Methylation at the N3, N7 and O6 sites of guanine, N1, N3 and N7 sites of adenine, O2 and N3 sites of cytosine and the O2 and O4 sites of thymine were considered. The computed reactivities for methylation follow the order N7(guanine)>N3(adenine)>O6(guanine) which is in agreement with experiment. The base pairing in DNA is found to play a significant role with regard to reactivities of the different sites.

2011-01-01

305

Environmental data for sites in the National Solar Data Network

Derivation of guidelines for uranium residual radioactive material in soil at the B&T Metals Company site, Columbus, Ohio

The Detartment of Energy's National Solar Data Program established solar energy systems in residential and commercial buildings across the United States. These solar sites are linked to Vitro Laboratories Division's computer in the National Solar Data Network (NSDN). Vitro collects and analyzes data from this network to determine the thermal performance of each of the solar systems. Environmental information collected at the sites for the month of August 1980 is presented. The environmental data for the NSDN are presented in the form of tables for each solar site. The solar sites are grouped into 12 zones, each of which consists of several adjacent states. The solar energy sites are in alphabetical sequence within each zone. The tables provide available meteorological data for reporting sites in the NSDN as follows: (1) Insolation: the ...

1980-08-01

306

Working paper on candidate mining sites for surface mining of western oil shales

Guidelines for uranium residual radioactive material in soil were derived for the B&T Metals Company site in Columbus, Ohio. This site has been identified for remedial action under the US Department of Energy`s (DOE`s) Formerly Utilized Sites Remedial Action Program (FUSRAP). Single-nuclide and total-uranium guidelines were derived on the basis of the requirement that following remedial action, the 50-year committed effective dose equivalent to a hypothetical individual living or working in the immediate vicinity of the site should not exceed a dose constraint of 30 mrem/yr for the current use and likely future use scenarios or a dose limit of 100 n-mrem/yr for less likely future use scenarios. The DOE residual radioactive material guideline computer code, RESRAD, was used in this evaluation. RESRAD implements the methodology described in the DOE manual for establishing residual radioactive material ...

1996-01-01

307

Superfund Record of Decision (EPA Region 1): Baird and McGuire, MA. (Third remedial action), September 1989

This report describes the selection of candidate surface mining sites for the development of oil shale resources in the western United States. The purpose of this study is to input the various scenarios into an economic mine model developed for underground oil shale mines in an earlier project. Site selection was a three-phase effort. Phase I consisted of a thorough review of available resource data, which resulted in a definition of surface mineable areas. Phase II was a detailed investigation of the regions identified in the initial phase. The end result of this task was a list of potential sites having favorable overburden, grade, shale thickness, and topography values. Phase III identified those sites from which a wide range of site specific parameters can be obtained for mine design and model testing purposes. This report describes the procedures used to obtain the candidate ...

1981-09-02

308

Site characterization and petroleum hydrocarbon plume mapping

The Baird and McGuire site is a former chemical manufacturing facility in northwest Holbrook, Massachusetts, approximately 14 miles south of Boston. From 1912 to 1983 the company operated a chemical manufacturing and batching facility on the property. Manufactured products included herbicides, pesticides, disinfectants, soaps, floor waxes and solvents. Waste disposal methods at the site included direct discharge into the soil, nearby brook and wetlands, and a former gravel pit (now covered) in the eastern portion of the site. EPA conducted a removal action at the site in 1983 after a waste lagoon overflowed spreading contaminants into the Cochato River. The company ceased operating shortly thereafter. A second removal action was conducted in 1985, following the discovery of dioxin in site soils. EPA also conducted an Initial Remedial Measure at the site from ...

1989-09-14

309

Should high-level nuclear waste be disposed of at geographically dispersed sites?

This paper presents a case study of site characterization and hydrocarbon contamination plume mapping/delineation in a gas processing plant in southern Mexico. The paper describes innovative and cost-effective use of passive (non-intrusive) and active (intrusive) techniques, including the use of compound-specific analytical methods for site characterization. The techniques used, on a demonstrative basis, include geophysical, geochemical, and borehole drilling. Geochemical techniques used to delineate the horizontal extent of hydrocarbon contamination at the site include soil gas surveys. The borehole drilling technique used to assess the vertical extent of contamination and confirm geophysical and geochemical data combines conventional hollow-stem auguring with direct push-probe using Geoprobe. Compound-specific analytical methods, such as hydrocarbon fingerprinting and a modified method for gasoline range organics, ...

1996-12-31

310

Public involvement in the siting of contentious facilities; Lessons from the radioactive waste repository siting programmes in Canada and the United States, with special reference to the Swedish repository siting process

Consideration of the technical feasibility of Yucca Mountain in Nevada as the site for a high-level nuclear waste repository has led to an intense debate regarding the economic, social, and political impacts of the repository. Impediments to the siting process mean that the nuclear waste problem is being resolved by adhering to the status quo, in which nuclear waste is stored at scattered sites near major population centers. To assess the merits of alternative siting strategies--including both the permanent repository and the status quo- we consider the variables that would be included in a model designed to select (1) the optimal number of disposal facilities, (2) the types of facilities (e.g., permanent repository or monitored retrievable facility), and (3) the geographic location of storage sites. The objective function in the model is an all-inclusive measure of social cost. The ...

1992-07-01

311

Mechanism of adenylate kinase. Dose adenosine 5'-triphosphate bind to the adenosine 5'-monophosphate site

This report describes the conclusion of a two-part programme, begun in 1994 with the overall aim of assisting in the development of an acceptable public participation strategy for use in Sweden for the siting of contentious facilities, with particular reference to the ongoing siting programme for a deep repository for spent nuclear fuel. The first part of the work programme, a global review of siting practice, was reported as SSI Rapport 94-15, in November 1994. This recommended further detailed studies of at least two individual programmes, which have now been carried out in Canada and the United States, and are reported on here. They involved face to face meetings with many of the main stake holders in the two programmes and enabled valuable insight to be gained into the potential problems associated with increased public participation, as well as identifying good practice where it exists. The lessons learned have then ...

1997-08-01

312

Fluxnet Synthesis Dataset Collaboration Infrastructure

Although the subtrate binding properties of adenylate kinase (AK) have been studied extensively by various biochemical and biophysical techniques, it remains controversial whether uncomplexed adenosine 5'-triphosphate (ATP) binds to the adenosine 5'-monophosphate (AMP) site of AK. The authors present two sets of experiments which argue against binding of ATP to the AMP site. (a) /sup 31/P nuclear magnetic resonance titration of ATP with AK indicated a 1:1 stoichiometry on the basis of changes in coupling constants and line widths. This ruled out binding of ATP to both sites. (b) ATP and MgATP were found to behave similarly by protecting AK from spontaneous inactivation while AMP showed only a small degree of protection. Such inactivation could also be protected or reversed by dithioerythritol and is most likely due to oxidation of sulfhydryl groups, one of which (cysteine-25) is located near the MgATP ...

1987-10-06

313

Remedial action selection report Maybell, Colorado, site. Final report

The Fluxnet synthesis dataset originally compiled for the La Thuile workshop contained approximately 600 site years. Since the workshop, several additional site years have been added and the dataset now contains over 920 site years from over 240 sites. A data refresh update is expected to increase those numbers in the next few months. The ancillary data describing the sites continues to evolve as well. There are on the order of 120 site contacts and 60proposals have been approved to use thedata. These proposals involve around 120 researchers. The size and complexity of the dataset and collaboration has led to a new approach to providing access to the data and collaboration support and the support team attended the workshop and worked closely with the attendees and the Fluxnet project office to define the requirements for the support infrastructure. As a result ...

2008-02-06

314

Evaluation of 1-site and 5-site models of methane on its adsorption on graphite and in graphitic slit pores.

The Maybell uranium mill tailings site is 25 miles (mi) (40 kilometers [km]) west of the town of Craig, Colorado, in Moffat County, in the northwestern part of the state. The unincorporated town of Maybell is 5 road mi (8 km) southwest of the site. The site is 2.5 mi (4 km) northeast of the Yampa River on relatively flat terrain broken by low, flat-topped mesas. U.S. Highway 40 runs east-west 2 mi (3.2 km) south of the site. The designated site covers approximately 110 acres (ac) (45 hectares [ha]) and consists of a concave-shaped tailings pile and rubble from the demolition of the mill buildings buried in the former mill area. The site is situated between Johnson Wash to the east and Rob Pit Mine to the west. Numerous reclaimed and unreclaimed mines are in the immediate vicinity. Aerial photographs (included at the end of this executive summary) show evidence ...

1996-12-01

315

Elevated standard metabolic rate in a freshwater shrimp (Palaeomonetes paludosus) exposed to trace element-rich coal combustion waste

In this paper, we evaluate the performance of the 1- and 5-site models of methane on the description of adsorption on graphite surfaces and in graphitic slit pores. These models have been known to perform well in the description of the fluid-phase behavior and vapor-liquid equilibria. Their performance in adsorption is evaluated in this work for nonporous graphitized thermal carbon black, and simulation results are compared with the experimental data of Avgul and Kiselev (Chemistry and Physics of Carbon; Dekker: New York, 1970; Vol. 6, p 1). On this nonporous surface, it is found that these models perform as well on isotherms at various temperatures as they do on the experimental isosteric heat for adsorption on a graphite surface. They are then tested for their performance in predicting the adsorption isotherms in graphitic slit pores, in which we would like to explore the effect of confinement on the molecule packing. Pore widths of 10 and 20 A are chosen in this ...

2005-10-20

316

Sequential outbreaks of multidrug-resistant Acinetobacter baumannii in intensive care units of a tertiary referral hospital in Italy: combined molecular approach for epidemiological investigation

A transplant experiment was conducted to determine whether standard metabolic rate (SMR) of a freshwater shrimp (Palaeomonetes paludosus) would be affected by exposure to trace element-enriched coal combustion waste (coal ash). Shrimp were transplanted into replicate cages in a coal ash-polluted site and a reference site for 8 months. The coal ash-polluted site was characterized by elevated sediment concentrations of As, Cd, Cr, Cu, Ni, Pb and Se compared to sediments in the reference site. After 8 months in the study sites, shrimp in the polluted site appeared to have accumulated As, Cd and Se from the habitat, but there were on differences in survival between the study sites. However, mean SMR of shrimp (measured as O{sub 2} consumption at rest) held in the polluted site was 51% higher than mean SMR of shrimp held in ...

1998-12-01

317

British Library Electronic Table of Contents (United Kingdom)

SummaryA laboratory-based surveillance study was conducted from January 2007 to May 2010 in San Martino Tertiary Referral Hospital in Genoa, Italy in which the molecular epidemiology of multidrug-resistant Acinetobacter baumannii was investigated in the five intensive care units (ICUs). A total of 53 A. baumannii strains were isolated from patients admitted to ICUs (69.8%) and to other epidemiologically linked hospital wards (30.2%) and were genotyped by repetitive extragenic palindromic polymerase chain reaction (REP-PCR), multilocus sequence typing (MLST) and adeB sequence typing. REP-PCR fingerprinting analysis, MLST and adeB typing results were well correlated and allowed us to classify strains causing epidemic events into three major epidemic clones: A (REP-I/ST4, adeB-STII genotype) ...

2011-01-01

318

Regeneration of filaments (colonies) from Anabaena variabilis spheroplasts

Plant protection i willow plantations - Insects. Final report; Vaextskydd i Salixodlingar - Insekter. Slutrapport

A simple method for regeneration of filaments (clones) from spheroplasts of the cyanobacterium (blue-green alga), Anabaena variabilis, was developed and used to quantify cell growth in the presence of two antibiotics. Cells from exponential phase cultures of ATCC 29413 and M3 were harvested and incubated with lysozyme (0.12% in 0.03M K-phosphate, pH 6.8, 0.55M sorbitol; 37 C) to produce spheroplasts. The spheroplasts were washed with buffer, plated onto soft agar and incubated (18 h light: 6 hr dark, 27 C). Colonies became visible at 7 - 9 days and were monitored for times up to 21 days. The concentration of chloramphenicol which inhibited cell growth by 50% was approximately 1.8 mg ml/sup -1/ medium and the concentrations of ampicillin which inhibited cell growth by 50% were approximately 4 and 15 pg ml/sup -1/ medium for ATCC 29413 and M3, respectively. This method may be useful for genetic manipulation of cells from these and other filamentous, N/sub 2/-fixing ...

1986-04-01

319

Physical mapping of human chromosome 16. Annual progress report

Attacks by herbivorous insects reduce biomass production in willow plantations. The long-term goal of our research is to develop sustainable plant protection against these insect pests. Two lines of research are pursued. The first concentrates on identifying factors involved in the regulation of leaf-eating beetles. These insects vary in density among plantations, sometimes reaching outbreak levels resulting in complete defoliation. Mortality factors of eggs and larvae in plantations with low and high beetle densities have been investigated. The importance of plant resistance has also been investigated. The second line of research considers the mechanisms behind an earlier demonstrated resistance in certain willow clones against a gall midge. By using molecular techniques we have searched for markers of resistance. Promising results have been found concerning so called PR proteins; in particular chitinase seems to be present in high activities in plants expressing ...

2000-04-01

320

Multistep process of neoplastic transformation of normal human fibroblasts by 60Co gamma rays and Harvey sarcoma viruses

We aim to isolate cDNAs mapping to human chromosome 16 and localise such cDNAs on the high resolution physical map. In collaboration with LANL, PCR primers will be synthesised from cDNA sequences mapped to chromosome 16 and used as ESTs in the generation of mega-YAC contigs for this chromosome. Probing of high density cosmid grids will enable integration of the ESTs into cosmid contigs and location of the cosmid contigs on the YAC contig. A hn-cDNA library has been constructed from the hybrid CY18 which contains chromosome 16 as the only human chromosome. A modified screening protocol has been successfully developed and 15 hn-cDNA clones have been sequenced and localised on the hybrid map. Sequence analysis of four of these revealed that they were known cDNAs, which are now mapped to chromosome 16. Development of techniques to allow the isolation of longer cDNAs from the identified exons is in progress. This will depend on PCR amplification of cDNAs from a total ...

1993-08-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

Microaerophilic growth and induction of the photosynthetic reaction center in Rhodopseudomonas viridis

As reported previously (Namba et al., 1985), normal human fibroblasts were transformed by 60Co gamma-ray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST-6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha-MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha-MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha-MSV-transformed KMST-6 cells showed an enhanced expression of the ras oncogene, but normal and 60Co gamma-ray-transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha-MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one ...

1986-03-15

322

Mapping of the gene encoding the melanocortin-1 ([alpha]-melanocyte stimulating hormone) receptor (MC1R) to human chromosome 16q24. 3 by fluorescence in situ hybridization

Rhodopseudomonas viridis was grown in liquid culture at 30 degrees C anaerobically in light (generation time, 13 h) and under microaerophilic growth conditions in the dark (generation time, 24 h). The bacterium could be cloned at the same temperature anaerobically in light (1 week) and aerobically in the dark (3 to 4 weeks) if oxygen was limited to 0.1%. Oxygen could not be replaced by dimethyl sulfoxide, potassium nitrate, or sodium nitrite as a terminal electron acceptor. No growth was observed anaerobically in darkness or in the light when air was present. A variety of additional carbon sources were used to supplement the standard succinate medium, but enhanced stationary-phase cell density was observed only with glucose. Conditions for induction of the photosynthetic reaction center upon the change from microaerophilic to phototrophic growth conditions were investigated and optimized for a mutant functionally defective in phototrophic growth. R. viridis ...

1989-05-01

323

Increased transcription of the c-myc oncogene in two methylcholanthrene-induced quail fibroblastic cell lines

[alpha]-Melanocyte stimulating hormone ([alpha]-MSH), a hormone originally named for its ability to regulate pigmentation of melanocytes, is a 13-amino-acid post-translational product of the pro-opiomelanocortin (POMC) gene. [alpha]-MSH and the other products of POMC processing, which share the core heptapeptide amino acid sequence Met-Glu (Gly)-His-Phe-Arg-Trp-Gly (Asp), the adrenocorticotropic hormone (ACTH), [beta]-MSH, and [gamma]-MSH, are collectively referred to as melanocortins. While best known for their effects on the melanocyte (pigmentation) and adrenal cortical cells (steroidogenesis), melanocortins have been postulated to function in diverse activities, including enhancement of learning and memory, control of the cardiovascular system, analgesia, thermoregulation, immunomodulation, parturition, and neurotrophism. To identify the chromosomal band encoding the human melanocortin-1 receptor gene, 1 [mu]g of an EMBL clone coding region of the human MC1R ...

1994-01-15

324

Increased ethanol resistance in Ethanolic Escherichia coli by Insertion of heat-shock genes BEM1 and SOD2 from Saccharomyces cerevisiae

The expression of three c-onc genes (c-erb, c-myc, c-myb) was investigated in five cell lines established from fibrosarcomas induced with 20-methylcholanthrene (MCA) of Japanese quails. These cell lines showed low levels of the three c-onc genes, with the exception of two cell lines that accumulated moderate (MCAQ 1-4) and large amounts (MCAQ 3-5) of c-myc RNA. Molecular cloning and restriction endonuclease analyses indicated that expression of c-myc in these two cell lines were not associated with detectable rearrangements in the c-myc locus, that the size of the c-myc transcript (2.7 kb) in MCAQ 3-5 was similar to that of the normal c-myc messenger RNAs (mRNA) and that the transcriptional activatin observed in MCAQ 3-5 was not mediated by the LTR (long terminal repeat) of a proximate ALV (avian leukosis virus) provirus. Finally, when analyzed with the restriction enzymes Msp I and Hpa II, the c-myc locus of MCAQ 3-5 and MCAQ 1-4 was found hypomethylated as ...

1984-12-01

325

British Library Electronic Table of Contents (United Kingdom)

Ethanol is generally toxic to microorganisms, and intracellular and extracellular accumulation of ethanol inhibits cell growth and metabolism. In this study, pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) were cloned into pET-32a vector and then introduced into E. coli BL21 to produce ethanol. Heat shock genes (BEM1 and SOD2) from Saccharomyces cerevisiae were inserted into recombinant ethanolic E. coli using pET28_a vector to improve ethanol shock resistance. Three different strains were constructed: Ethanolic E. coli (adhB and pdc genes inserted using pET32_a vector), BEM1 gene-inserted E. coli (BEM1 inserted using pET_28a), and SOD2-inserted E. coli (SOD2 inserted using pET28_a). Construction of these three different strains allowed comparison of the functions of these he...

2010-01-01

326

Human tissue factor pathway inhibitor (TFPI) gene: Complete genomic structure and localization on the genetic map of chromosome 2q

Heat shock factor binds to heat shock elements upstream of heat shock protein 70a and Samui genes to confer transcriptional activity in Bombyx mori diapause eggs exposed to 5^oC

Tissue factor pathway inhibitor (TFPI), a protease inhibitor that circulates in association with plasma lipoproteins (VLDL, LDL and HDL), helps to regulate the extrinsic blood coagulation cascade. The authors have cloned a 125-kb genomic region containing the entire human TFPI gene on six overlapping cosmids and prepared a restriction map of this contig to clarify gene structure. More than half (45 kb) of the 85-kb gene is occupied with 5[prime] noncoding elements: coding begins at exon 3. A HindIII RFLP identified with one cosmid was genotyped in the CEPH panel of 559 reference families. Linkage analysis using markers on human chromosome 2 located the TFPI gene on 2q, 36 cM proximal to D2S43(pYNZ15) and 13 cM distal to the crystalline [gamma]-polypeptide locus CRYGP1(p5G1). 31 refs., 3 figs., 3 tabs.

1993-08-01

327

British Library Electronic Table of Contents (United Kingdom)

To understand the molecular mechanisms of how 5^oC-incubation activates mRNA expression of Hsp70a and Samui genes in Bombyx mori diapause eggs, we first searched the 5'-upstream regions of the Hsp70a and Samui genes for heat shock elements (HSEs) and found two regions [Hsp70aHSE-1 (-95 to -58) and -2 (-145 to -121), and SamuiHSE-1 (-84 to -55) and -2 (-304 to -290)] corresponding to HSEs (repeats of nGAAn and/or nTTCn). We cloned four cDNAs encoding heat shock factor (HSF)-a2 (627 amino acids), -b (685 aa), -c (682 aa) and -d (705 aa), which were produced by alternative splicing. When we exposed diapause eggs to 5^oC beginning at 2 day post-oviposition to break diapause, HSFd mRNA only increased after chilling for 6-8 days, a pattern very similar to those of Hsp70a and Samui mRNAs. To exam...

2011-01-01

328

Gap-junctional communication of bone marrow stromal cells is resistant to irradiation in vitro

Gap-junctional communication of bone marrow stromal cells is resistant to irradiation in vitro

Bone marrow is one of the most radiosensitive organs. Irradiation causes a marked decrease in the total number of hematopoietic cells in the bone marrow. The reticular meshwork structure of marrow stromal cells, however, is relatively resistant to irradiation. Unimpaired stromal cell structure has been thought to be a prerequisite for the repopulation of hematopoietic cells during recovery from the effects of irradiation. The reticular framework is maintained by cell adhesion apparatuses such as gap junctions. The in vitro radiobiologic survival values of a cloned stromal cell line, H-1/A, were studied (n = 1.8, D0 = 138 cGy). Radiation doses of up to 4000 cGy had no detectable effects on the production of colony-stimulating factor 1. H-1/A cells communicate with each other via gap junctions as determined by the sensitive dye-transfer method. Gap-junctional communication between H-1/A cells was resistant to different levels of irradiation (500 to 10,000 cGy), but ...

1990-10-01

329

Fiscal 1998 industrial science and technology R and D project. Research report on R and D of genome informatics technology (Development of stable oil supply measures using complex biosystem); 1998 nendo genome informatics gijutsu kenkyu kaihtsu seika hokokusho. Fukugo seibutsukei riyo sekiyu antei kyokyu taisaku kaihatsu

Bone marrow is one of the most radiosensitive organs. Irradiation causes a marked decrease in the total number of hematopoietic cells in the bone marrow. The reticular meshwork structure of marrow stromal cells, however, is relatively resistant to irradiation. Unimpaired stromal cell structure has been thought to be a prerequisite for the repopulation of hematopoietic cells during recovery from the effects of irradiation. The reticular framework is maintained by cell adhesion apparatuses such as gap junctions. The in vitro radiobiologic survival values of a cloned stromal cell line, H-1/A, were studied (n = 1.8, D0 = 138 cGy). Radiation doses of up to 4000 cGy had no detectable effects on the production of colony-stimulating factor 1. H-1/A cells communicate with each other via gap junctions as determined by the sensitive dye-transfer method. Gap-junctional communication between H-1/A cells was resistant to different levels of irradiation (500 to 10,000 cGy), but ...

330

Biotechnology developments in the livestock sector in developing countries.

This report describes the fiscal 1998 result on development of genome informatics technology. As comparative analysis technique of genes, the combination of electrophoresis and PCR was used. For improvement of the throughput and reproducibility of the technique, module- shuffling primers were used, and the multi(96)-arrayed capillary fragment analyzer was devised. The system detecting SNPs rapidly was also developed successfully. As analysis technology of DNA sequence by use of triple- stranded DNA formation, study was made on construction of long cDNA libraries, selective subtraction of specific sequences from libraries, and the basic technology of homologous cloning. Study was also made on each reaction step of IGCR technique for fast analysis, and specifications of a fluorescence transfer monitor. As modeling technique of genetic sequence information, the simulation model was developed for gene expression regulatory networks during muscle differentiation, and ...

1999-03-01

331

Analysis of the 5{prime} region of PMS2 reveals heterogeneous transcripts and a novel overlapping gene

Global meat and milk consumption is exponentially increasing due to population growth, urbanization and changes in lifestyle in the developing world. This is an excellent opportunity for developing countries to improve the livestock sector by using technological advances. Biotechnology is one of the avenues for improved production in the "Livestock revolution". Biotechnology developments applied to livestock health, nutrition, breeding and reproduction are improving with a reasonable pace in developing countries. Simple bio-techniques such as artificial insemination have been well implemented in many parts of the developing world. However, advanced technologies including transgenic plant vaccines, marker assisted selection, solid state fermentation for the production of fibrolytic enzymes, transgenic fodders, embryo transfer and animal cloning are confined largely to research organizations. Some developing countries such as Taiwan, China and Brazil have considered ...

2010-01-01

332

Analysis of forward mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in the bacteriophage P22 mnt repressor gene

The PMS2 gene encodes a protein that is involved in DNA mismatch repair and is mutated in a subset of patients with hereditary nonpolyposis colon cancer (HNPCC). The previously published PMS2 cDNA sequence lack an upstream in-frame stop codon preceding the presumptive initiating methionine. To evaluate the 5` terminus of the PMS2 coding region further, we isolated additional cDNA clones, RT-PCR products, and the corresponding 5` genomic segment of the PMS2 locus. The PMS2 gene transcripts were found to have heterogeneous but colinear 5` termini, one of which contained an in-frame termination codon preceding the initiating methionine. In addition, a novel gene encoding a 34.5-kDa polypeptide was found to initiate transcriptionally within PMS2 from the opposite strand. 23 refs., 5 figs., 2 tabs.

1995-09-20

333

A YAC contig encompassing the recessive Stargardt disease gene (STGD) on chromosome 1p

We describe the isolation and genetic characterization of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutations in the phage P22 mnt repressor gene cloned in plasmid pBR322. Mutations in the mnt repressor gene or its operator on this plasmid, pPY98, confer a tetracycline resistance phenotype, whereas the wild-type plasmid confers tetracycline sensitivity. Cells carrying pPY98 were briefly exposed to MNNG to give 20 to 40% survival and a 50- to 100-fold increase in tetracycline-resistant cells. DNA sequence analysis showed that 29 to 30 MNNG-induced mutations were GC-to-AT transitions and one was an AT-to-GC transition. About 80% of the mutations are in three hotspots. This mutation spectrum is consistent with the proposed mechanism of mutagenic action of MNNG, which involves mispairing of an alkylated base, O/sup 6/-methylguanine. The mnt gene may be a useful target for determining mutagenic specificity at the nucleotide level because (i) forward ...

1986-04-01

334

Structure and magnetic properties of the Al1-xGaxFeO3 family of oxides: A combined experimental and theoretical study

Stargardt disease (STGD) and fundus flavimaculatus are infrequent autosomal recessive conditions characterized by a juvenile macular dystrophy and variable degrees of peripheral retinal changes. Linkage analysis performed in 47 STGD/fundus flavimaculatus families demonstrated significant linkage to 13 polymorphic DNA markers on chromosome 1p. The maximum combined two-point lod score was 32.7 (maximum recombination fraction [{theta}{sub max}] = .006) with the polymorphic marker D1S188. Our data demonstrate that STGD and fundus flavimaculatus are the same disorder clinically and genetically and provide further evidence for genetic homogeneity of this phenotype. Analysis of recombination on disease chromosomes placed the STGD gene within a 4-cM interval between markers D1S435 and D1S236. A physical map was constructed of a YAC contig flanking STGD, from markers D1S500 to D1S495, and includes the critical interval delineated by historical recombinants. This contig spans {approximately}31 ...

1995-12-01

335

Photocatalytic probing of DNA sequence by using TiO{sub 2}/dopamine-DNA triads.

Magnetic properties of the Al1-xGaxFeO3 family of oxides crystallizing in a non-centrosymmetric space group have been investigated in detail along with structural aspects by employing X-ray and neutron diffraction, Moessbauer spectroscopy and other techniques. The study has revealed the occurrence of several interesting features related to unit cell parameters, site disorder and ionic size. Using first-principles density functional theory based calculations, we have attempted to understand how magnetic ordering and related properties in these oxides depend sensitively on disorder at the cation site. The origin and tendency of cations to disorder and the associated properties are traced to the local structure and ionic sizes. -- Graphical abstract: We have studied both experimentally and theoretically the important role of disorder at the cation site on magnetic and related properties of the Al1-xGaxFeO3 family of oxides ...

2011-03-01

336

Overview of the programme forseen for the potential site Oberbauenstock (Community Bauen UR)

A method to control charge transfer reaction in DNA using hybrid nanometer-sized TiO{sub 2} nanoparticles was developed. In this system extended charge separation reflects the sequence of DNA and was measured using metallic silver deposition or by photocurrent response. Light-induced extended charge separation in these systems was found to be dependent on the DNA-bridge length and sequence. The yield of photocatalytic deposition of silver was studied in systems having GG accepting sites imbedded in AT runs at varying distances from the TiO{sub 2} nanoparticle surface. Weak distance dependence of charge separation indicative of a hole hopping through mediating adenine (A) sites was found. The quantum yield of silver deposition in the system having a GG accepting site placed 8.5 {angstrom} from the nanoparticle surface was found to be {Phi} = 0.70 (70%) and {Phi} = 0.56 (56%) for (A){sub n} and (AT){sub n/2} bridge, ...

2007-10-15

337

Obsidian from volcanic sequences and rent alluvial deposits, Erzurum district, north-eastern Anatolia: Chemical characterisation and archaeological implications

On 30th September 1985, the Federal Government licensed site suitability investigations in three areas with a view to disposal of low-and intermediate-level radioactive waste. This report gives a summary overview of the corresponding research programme at the potential site Oberbauenstock. The first part of the report begins with some comments on the concept of radioactive waste disposal and on some basic aspects of site-specific exploration and investigation. The governmental decision of 30th September and Nagra's two-phase investigation concept are then briefly considered. To close, the objectives of investigation phase I are outlined and possible results are briefly discussed. The research programme developed for investigation of the potential site at Oberbauenstock is presented in the second part of the report. Besides geological and hydrogeological investigations at the surface, the programme ...

1985-09-01

338

Identification of protein phosphorylation sites within Ser/Thr-rich cluster domains using site-directed mutagenesis and hybrid linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry

Full text: Recent geoarchaeological research in the Erzurum district, north-eastern Anatolia, has revealed an abundance of obsidian at numerous neolithic and Bronze Age sites. Geochemical characterisation using neutron activation analysis indicates that the obsidian was obtained from several sources that are chemically distinct from the major sources already known from Central Anatolia an the Lake Van area. Multiple sources are represented in the samples collected from at least two of the sites, namely the sites of Sos and Pulur. The primary source of some of the obsidian utilised at the site of Sos has been located in the volcanic sequence outcropping tot he north-west of Pasinler. Field survey however has shown that the alluvial deposits along the main rivers and some of their tributaries were the main sources of the obsidian utilised at the sites near Erzurum. Trade or exchange ...

1997-12-31

339

British Library Electronic Table of Contents (United Kingdom)

We describe a method for the analysis of multi-site phosphorylation in serine/threonine (Ser/Thr)-rich protein sequences. Site-specific mutagenesis was used to introduce tryptic cleavage sites in the serine glutamine/threonine glutamine cluster domain (SCD) of the human checkpoint protein kinase (Chk2). The mutant proteins were shown to autophosphorylate on residues that are inducibly phosphorylated when mammalian cells are exposed to ionizing radiation (serine 33/35, serine 516, threonine 68 and threonine 432). Five Ser/Thr clusters within the SCD were flanked by arginine or lysine residues to produce tryptic peptides for nanospray liquid chromatography (nanoLC)/linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Phosphorylation sites were assigned usin...

2007-01-01

340

Behaviour of the de-inked tailings soil cover at the Clinton mining site; Comportement de la couverture de residus de desencrage au site minier Clinton

[Experimental and kinetic modeling of acid/base and redox reactions over oxide catalysts

The production of de-inked tailings in Quebec has significantly increased during the last decade. From less than 100,000 wet tons in 1990, the production of de-inked tailings reached 500,000 wet tons in 1995, and 510,000 wet tons in 1999. The geotechnical, hydraulic, and physico-chemical properties of de-inked tailings are favorable for its use as soil cover for the prevention of acid mine drainage at mining sites. In this presentation, the authors discussed the three-year monitoring of the behaviour of a membrane covering an old mining site with the potential to produce acid mine drainage. The site is located at the Clinton Mine, near Woburn, Quebec. The mine shaft was filled with the tailings and waste rock scattered around the site and covered with a protective membrane which included de-inked tailings. The profile of the membrane was discussed, as well as construction details. The results confirmed ...

2000-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

Using Multiple Unmanned Systems for a Site Security Task

The research has involved the characterization of catalyst acidity, [sup 2]D NMR studies of Bronsted acid sites, and kinetic, calorimetric, and spectroscopic studies of methylamine synthesis and related reactions over acid catalysts. Approach of this work was to explore quantitative correlations between factors that control the generation, type, strength, and catalytic properties of acid sites on zeolite catalysts. Microcalorimetry, thermogravimetric analysis, IR spectroscopy, and NMR spectroscopy have provided information about the nature and strength of acid sites in zeolites. This was vital in understanding the catalytic cycles involved in methylamine synthesis and related reactions over zeolite catalysts.

1993-01-01

342

Trends and Controls on Summer Surface-Water Temperatures in Salmonid-Bearing Headwater Streams in Two Common Geomorphic Settings, Kenai Peninsula, Alaska

Unmanned systems are often used to augment the ability of humans to perform challenging tasks. While the value of individual unmanned vehicles have been proven for a variety of tasks, it is less understood how multiple unmanned systems should be used together to accomplish larger missions such as site security. The purpose of this paper is to discuss efforts by researchers at the Idaho National Laboratory (INL) to explore the utility and practicality of operating multiple unmanned systems for a site security mission. This paper reviews the technology developed for a multi-agent mission and summarizes the lessons-learned from a technology demonstration.

2009-04-01

343

Signatures of testing: On-site inspection technologies

Stream temperature is an important physical characteristic of headwater streams that plays a critical role in the presence and health of juvenile salmonids. Headwater stream temperature was documented in two geomorphic settings on the Kenai Peninsula, Alaska, focusing on the variation in temperature induced by diffuse groundwater discharge and variable air temperature. Eighteen headwater stream reaches were studied in four watersheds, with 11 drainageway sites and seven discharge-slope sites. In drainageway sites, low-gradient streams flow through broad valleys with groundwater-fed fen wetlands; in discharge-slope sites, high-gradient streams flow through narrow valleys with groundwater-fed slope wetlands. At all 18 sites, hourly stream temperatures were measured for one year. At one drainageway and one discharge-slope site, groundwater temperatures, stream ...

2010-12-01

344

Pre-waste-emplacement ground-water travel time sensitivity and uncertainty analyses for Yucca Mountain, Nevada; Yucca Mountain Site Characterization Project

This paper describes the phenomenology of nuclear explosions and technologies for their detection as relevant to On-Site Inspection (OSI) for a comprehensive test-ban (CTB). Our experience with the US nuclear test program which has been primarily carried out at the Nevada Test Site (NTS) and in the Pacific Ocean. The goals of OSI are to resolve ambiguous events, reduce uncertainty, deter attempts at evasion, and provide responsive and technically competent means of confirming the occurrence of a nuclear explosion should deterrence fail. These goals would include finding evidence of an evasive nuclear explosion or evidence that the event was non-nuclear, such as an earthquake or large chemical explosion.

1995-01-01

345

Ocean FUSRAP: Feasibility of ocean disposal of materials from the Formerly Utilized Sites Remedial Action Program (FUSRAP)

Yucca Mountain, Nevada is a potential site for a high-level radioactive-waste repository. Uncertainty and sensitivity analyses were performed to estimate critical factors in the performance of the site with respect to a criterion in terms of pre-waste-emplacement ground-water travel time. The degree of failure in the analytical model to meet the criterion is sensitive to the estimate of fracture porosity in the upper welded unit of the problem domain. Fracture porosity is derived from a number of more fundamental measurements including fracture frequency, fracture orientation, and the moisture-retention characteristic inferred for the fracture domain.

1993-01-01

346

Numerical simulation of trace tests in atmosphere in Daya Bay nuclear power site

The purpose of the Ocean FUSRAP Program, which began in March 1981, is to assess the technical, environmental, and institutional feasibility of disposing, in the ocean and on the ocean floor, of FUSRAP waste which contains traces of natural radioactive materials. The initial planning has focused on the Middlesex, New Jersey, Sampling Plant site and surrounding properties, which contain on the order of 100,000 metric tons of material. The Belgian Congo uranium ore and other uranium ores used by the United States were handled at the sampling plant site and have since been removed.

347

Implantation of single-impurity Fe and its magnetic coupling in Er studied by TDPAD

The validation of the forecast model for early emergency response to nuclear accidents is evaluated by trace tests in atmosphere in Daya Bay nuclear power site. The simulation experiment of the Daya Bay nuclear power site shows that the particle spreading image and the time-integrated concentration distribution given by plume concentration prediction model can perform the variation of pathway of the pollutant transport, as well as the effects of topography on transport and diffusion of pollutants. The simulation of five trace tests in field shows that 59.1% of ratios between predicted results and observed results are within the range of 10, and 41% of ratios are within the range of 5 approximately. (authors)

2005-09-01

348

Comparison and analysis of results of ? radiation level investigation around Daya Bay

Single Fe impurities were implanted in an Er single crystal and found to occupy both substitutional and interstitial sites, below a temperature of 200 K. The local susceptibility of Fe on both sites follows a Curie-Weiss law and exhibits a positive local Curie constant, indicating an antiferromagnetic coupling between the Fe and the surrounding Er moments. The corresponding nuclear spin relaxation rates follow a Korringa law as a function of temperature, confirming the dominance of local magnetism and the formation of local moments on each of the sites occupied by Fe.

2004-05-01

349

Beginning PHP, Apache, MySQL web development

Investigations were carried out of radiation level around Daya Bay for three times since the construction and operation Daya bay Nuclear Power Station and Lingao Nuclear Power Station in Guangdong province in the period of 1988 to 2000. As shown by the investigation, the radiation levels both in the field and indoors remains unchanged by and large but slightly higher than before on the roads. Also this paper gives the analysis of variations in radiation levels form site to site and from time to time. It was shown that, for environmental ? radiation levels measurements, typical measuring sites have significant impact on the meaning results. (authors)

2003-08-01

350

Bechtel Hanford Inc. Network Security Plan for the EnvironmentalRestoration Contract

An ideal introduction to the entire process of setting up a Web site using PHP (a scripting language), MySQL (a database management system), and Apache (a Web server)* Programmers will be up and running in no time, whether they're using Linux or Windows servers* Shows readers step by step how to create several Web sites that share common themes, enabling readers to use these examples in real-world projects* Invaluable reading for even the experienced programmer whose current site has outgrown the traditional static structure and who is looking for a way to upgrade to a more efficient, user-f

2004-01-01

351

An approach to rehabilitation objectives. Example of hydrocarbon polluted sites; Approche des objectifs de rehabilitation. Exemple des sites pollues par des hydrocarbures

'As part of the Computer Protection Plan, this Network Security Plan identifies the specific security measures used to protect Bechtel Hanford, Inc.'s (BHI's) enterprise network. The network consists of the communication infrastructure and information systems used by BHI to perform work related to the Environmental Restoration Contract (ERC) at the Hanford Site. It provides electronic communication between the ERC-leased facilities in Richland, Washington, and facilities located on the Hanford Site. Network gateways to other site and offsite networks provide electronic communication with the remainder of the Hanford community.'

1999-06-30

352

Work plan for the remedial investigation/feasibility study-environmental assessment for the Colonie site, Colonie, New York

The UFIP (French Petroleum Industry Association) studies concerning polluted sites and soils and their rehabilitation are presented: characterization of the admissible maximum fuel or gas oil content in the ground, the issue of time effects, and the risks it may induce for public health; study on the various pollutant impacts on the environment (underground waters, biotope...); approach to the development of a dynamical model describing these phenomena and their influence on man, in order to derive consistent and reasonable decontamination objectives

1996-12-31

353

Work plan addendum for the remedial investigation and feasibility study of the Salmon Site

This work plan has been prepared to document the scoping and planning process performed by the US Department of Energy (DOE) to support remedial action activities at the Colonie site. The site is located in eastern New York State in the town of Colonie near the city of Albany. Remedial action of the Colonie site is being planned as part of DOE's Formerly Utilized Sites Remedial Action Program. The DOE is responsible for controlling the release of all radioactive and chemical contaminants from the site. Under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), a remedial investigation/feasibility study (RI/FS) must be prepared to support the decision-making process for evaluating remedial action alternatives. This work plan contains a summary of information known about the site as of January 1988, presents a conceptual ...

1990-06-01

354

This document is intended as an addendum to the Remedial Investigation and Feasibility Study (RI/FS) Work Plan for the Salmon Site (SS) (formerly the Tatum Dome Test Site) Lamar County, Mississippi. The original work plan - Remedial Investigation and Feasibility Study of the Tatum Dome Test Site, Lamar County, Mississippi (herein after called the Work Plan) was approved by the state of Mississippi in 1992 and was intended as the operative document for investigative activities at the Tatum Dome Test Site. Subsequent to the approval of the document a series of activities were undertaken under the auspices of the work plan. This document is organized in the same manner as the original work plan: (1) Introduction; (2) Site Background and History; (3) Initial Evaluation; (4) Data Quality Objectives; (5) RI/FS Tasks; (6) Project Schedule; (7) Project Management; and (8) Reference. This ...

1995-11-01

355

Weather - Hanford Site

Waste reduction at the Savannah River Site

to Cool Spots Hanford Fun Facts Classroom Projects Famous People of Hanford Counterintelligence Home Employee Responsibilities CI Information CI Resources Points of Contact...

2011-10-01

356

Waste Food Storage at Fortress Rocks

The Savannah River Site (SRS) is a key installation for the production and research of nuclear materials for national defense and peace time applications and has been operating a full nuclear fuel cycle since the early 1950s. Wastes generated include high level radioactive, transuranic, low level radioactive, hazardous, mixed, sanitary, and aqueous wastes. Much progress has been made during the last several years to reduce these wastes including management systems, characterization, and technology programs. The reduction of wastes generated and the proper handling of the wastes have always been a part of the Site`s operation. This paper summarizes the current status and future plans with respect to waste reduction to waste reduction and reviews some specific examples of successful activities.

1990-12-31

357

Vulnerability of biomarkers in the indigenous mollusk Anodonta cygnea to spontaneous pollution in a transition country

... of waste food generated at McMurdo Station. Some of the difficulties of disposing of waste food ... change waste generation or management at the site? Yes. This will be a temporary waste management ...

358

British Library Electronic Table of Contents (United Kingdom)

The aim of this study was to estimate the sensitivity of biomarkers of stress and exposure in the bivalve mollusk Anodonta cygnea to spontaneous anthropogenic activities typical for the Western Ukraine. Three sites were examined during spring, summer and autumn: an agricultural site (A); the cooling pond of nuclear power plant (N) and a forestry close to the municipal water inlet (F). Common temporal changes of a battery of biochemical markers in the gills and hemolymph and morphological characteristics were shown by discriminant functional analysis. Classification trees built on the basis of the screened biomarkers demonstrated persistent peculiarities at each site: genotoxicity (nuclear abnormalities) at site A and endocrine disruption (high levels of vitellogenin-like proteins (Vtg-LP) ...

2010-01-01

359

Visitor Attitudes Towards Charging fees at Selected Sites within the Colorado Canyons National Conservation Area - ...

The hepcidin-binding site on ferroportin is evolutionarily conserved

... the Park Services Colorado Canyons National Conservation Area (CCNA)....

360

The Mitoscriptome in Aging and Disease

SummaryMammalian iron homeostasis is regulated by the interaction of the liver-produced peptide hepcidin and its receptor, the iron transporter ferroportin. Hepcidin binds to...Full Text Available

2008-08-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Summary of the engineering assessment of inactive uranium mill tailings, Spook Site, Converse County, Wyoming

Mitochondria are the major sites where energy is produced in the cell. Functions of organs such as the heart which has high energy demand are seriously affected by dysfunction of mitochondria....Full Text Available

2011-04-19

362

Subaqueous deposition of reactive mine tailings in an open trench; Deposition subaquatique de residus miniers reactifs dans une fosse a ciel ouvert

Ford, Bacon, Davis Utah Inc. has reevaluated the Spook site in order to revise the December 1977 engineering assessment of the problems resulting from the existence of radioactive uranium mill tailings 48 mi northeast of Casper, in Converse County, Wyoming. This engineering assessment has included the preparation of topographic maps, the performance of core drillings and radiometric measurements sufficient to determine areas and volumes of tailings and radiation exposures of individuals and nearby populations, the investigations of site hydrology and meteorology, and the evaluation and costing of alternative corrective actions. Radon gas released from the 187,000 tons of tailings at the Spook site constitutes the most significant environmental impact, although windblown tailings and external gamma radiation also are factors.

1981-10-01

363

Structure and Characterization of Nicotinic Acetylcholine ...

This poster presentation gave an overview of the subaqueous deposition of reactive mine tailings in the open trench Don Rouyn, located near Rouyn-Noranda, Quebec. The author began by providing a brief background of subaqueous mine tailing storage, and discussed the interest of the Don Rouyn trench. The site was described according to geomorphological and geological aspects. The hydrology conditions existing at the site were mentioned, and the results of the hydrology analysis performed were indicated. Close monitoring of the situation was maintained, and the chemical and physical stability of the tailings were evaluated. The conditions that prevailed both before and after the flooding of the site were explained. Water quality is being monitored, and the results obtained at this site might be used at other locations. 40 figs.

2000-07-01

364

Specialization and Omnivory in Diverse Mammalian Assemblages

... the ct-neurotoxin from Dendroaspis viridis venom (a-DTX) , which binds to four sites on the AcChR molecule (Conti-Tronconi & Raftery 1986). ...

1991-11-07

365

Spatial Relationships between Drug Binding Sites on the ...

... sur un site. Ces espèces présentent un vaste spectre de régimes alimentaires couvrant plusieurs niveaux trophiques et ... ...

366

Smoking and reproduction: The oviduct as a target of cigarette smoke

... determination of the molar extinction coefficients, e, were measured by the Lowry method with native a-toxin as a standard. Binding Kinetics. ...

1987-10-15

367

Site-Specific Methylation of the Promoter Alters Deoxyribonucleic Acid-Protein Interactions and Prevents ...

The oviduct is an exquisitely designed organ that functions in picking-up ovulated oocytes, transporting gametes in opposite directions to the site of fertilization, providing a suitable environment...Full Text Available

368

Remedial Action Plan and Site Design for Stabilization of the Inactive Uranium Mill Tailings Site, Maybell, Colorado. Remedial action selection report: Attachment 2, Geology report, Final

... and R. L. Momparler. DNA methylation of retinoic acid receptor beta in breast cancer and possible therapeutic role of ... ...

369

RADIONUCLIDE DATA PACKAGE FOR PERFORMANCE ASSESSMENT CALCULATIONS RELATED TO THE E-AREA LOW-LEVEL WASTE FACILITY AT THE SAVANNAH RIVER SITE.

The Maybell uranium mill tailings site is 25 miles (mi) (40 kilometers [km]) west of the town of Craig, Colorado, in Moffat County, in the northwestern part of the state. The unincorporated town of Maybell is 5 road mi (8 km) southwest of the site. The designated site covers approximately 110 acres (ac) (45 hectares [ha]) and consists of a concave-shaped tailings pile and rubble from the demolition of the mill buildings buried in the former mill area. Contaminated materials at the Maybell processing site include the tailings pile, which has an average depth of 20 feet (ft) (6 meters [m]) and contains 2.8 million cubic yards (yd{sup 3}) (2.1 million cubic meters [m{sup 3}]) of tailings. The former mill processing area is on the north side of the site and contains 20,000 yd{sup 3} (15,000 m{sup 3}) of contaminated demolition debris. Off-pile contamination is present and includes areas ...

1994-06-01

370

Postremediation dose assessment for the former Alba Craft Laboratory site, Oxford, Ohio

The Savannah River Site disposes of low-level radioactive waste within on-site engineered disposal facilities. The Savannah River Site must demonstrate that these disposals meet the requirements of DOE Order 435 . 1 through a process known as performance assessment (PA). The objective of this document is to provide the radionuclide -specific data needed for the PA calculations . This work is part of an on-going program to periodically review and update existing PA work as new data becomes available. Revision of the E -Area Low-Level Waste Facility PA is currently underway. The number of radionuclides selected to undergo detailed analysis in the PA is determined by a screening process. The basis of this process is described. Radionuclide-specific data for half-lives, decay modes, daughters, dose conversion factors and groundwater concentration limits are presented with source references and methodologies.

2007-03-20

371

Plan for addressing issues relating to oil shale plant siting

Potential maximum radiation dose rates were calculated for the former Alba Craft Laboratory site in Oxford, Ohio, which was involved in machining of uranium metal in the 1950s for the U.S. atomic energy program. The site is not currently being used. The residual radioactive material guidelines (RESRAD) computer code, which implements the methodology described in the US Department of Energy`s (DOE`s) manual for establishing residual radioactive material guidelines, was sued in this evaluation. Three potential land use scenarios were considered for the former Alba Craft site; the scenarios vary with regard to the type of site use, time spent at the site by the exposed individual, and sources of food consumed. Scenario A (a possible land use scenario) assumed industrial use of the site; Scenario B (a likely future land use scenario) assumed residential use of the ...

1996-04-01

372

PHOSIDA 2011: the posttranslational modification database

The Western Research Institute plan for addressing oil shale plant siting methodology calls for identifying the available resources such as oil shale, water, topography and transportation, and human resources. Restrictions on development are addressed: land ownership, land use, water rights, environment, socioeconomics, culture, health and safety, and other institutional restrictions. Descriptions of the technologies for development of oil shale resources are included. The impacts of oil shale development on the environment, socioeconomic structure, water availability, and other conditions are discussed. Finally, the Western Research Institute plan proposes to integrate these topics to develop a flow chart for oil shale plant siting. Western Research Institute has (1) identified relative topics for shale oil plant siting, (2) surveyed both published and unpublished information, and (3) identified data gaps and research ...

1987-09-01

373

The primary purpose of PHOSIDA (http://www.phosida.com) is to manage posttranslational modification sites of various species...Full Text Available

2011-01-01

374

Ocean Water: Density

Nuclear power plant personnel training in France

This site explains how temperature, pressure, and salinity work together to determine the density of ocean water. The three density layers of the ocean are described by means of text description and a graphic illustration.

375

National remediation program of radioactively contaminated sites in the Republic of Croatia

Data on French nuclear electricity generation sites, nuclear power plant operations personnel, operation simulators, nuclear training centers and training statistics are presented.

1994-03-21

376

National Interagency Fire Center

The national remediation program in Croatia consists of three stages: (1) identification and characterization of radioactively contaminated sites; (2) preparing activities for restoration; and (3) physical restoration at selected (high-prioritized) sites. The program is coordinated by the APO and, in the first stage, performed by both national research institutes of Ruder Boskovi and Institute for Medical Research and Occupational Health. The program supervisors are Ministry of Health, Ministry of Economy (Department of Energy) and State Administration for Environmental Protection. There were more than thirty sites suspected to be radioactively contaminated in Croatia. They were classified into four contamination type groups: (1) dumps containing contaminated coal slag and ash; (2) dumps containing contaminated phosphates and phospho-gypsum resulting from fertilizers industry; (3) geothermal springs and gas/oil wells; and ...

1995-12-31

377

Molecules | Special Issue: Neuroactive Compounds

and the Wildland Firefighter Foundation. To request a marker click here. Engraved bricks may also be purchased from the Foundation to become part of the site. To request a...

2011-07-22

378

Wastenet

... Here we will summarize the synthesis, structure activity relationships , and molecular sites of action of mGluR5 PAMs. We will also review preclinical studies ...

379

Microstructure and atomic effects on the electroluminescent efficiency of SrS:Ce thin film devices

Metallointercalators and Metalloinsertors

Transmission electron microscopy and x-ray diffraction data show that rapid thermal anneals of SrS:Ce thin films enhance grain size and reduce crystalline defects. Electron paramagnetic resonance results suggest that these anneals lead to less variance in the crystal field environments at the nearly cubic Ce"3"+ sites along with the formation of another type of Ce"3"+ site believed to involve a nearby Sr vacancy. We suggest that the association of Ce"3"+ sites with V_S_r shifts the electroluminescence towards larger wavelengths as the symmetry of the activator site is lowered. copyright 1997 American Institute of Physics.

380

Management approaches for improving environmental restoration at the Savannah River Site: Projectization, performance, and communications; Savannah River Site, Aiken, South Carolina

Since the elucidation of the structure of double helical DNA, the construction of small molecules that recognize and react at specific DNA sites has been an area of considerable interest. In...Full Text Available

2007-11-28

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Is It Time To Give The US Engineer Battalion (Combat) (Heavy ...

The purpose of this paper is to communicate how new and established management techniques are applied to environmental restoration projects at the Savannah River Site. Specifically, the paper discusses application of four (4) management approaches: Total Quality Principles; Task Team Structure; Cost Time Management; SAFER (Streamlined Approach for Environmental Restoration). The objective is to share Savannah River Site experience and document case studies where certain approaches have enhanced projects at hand. Each management approach is demonstrated by its project application and impact on performance. The visibility given the project is discussed to emphasize communications as avenues for public information, technical exchange, and employee motivation.

1993-02-01

382

Implementation of an Intravenous/Phlebotomy Team at Walter ...

... revetment construction, water well drilling, mobile facility assets construction using ... five days, and establish site layout for the follow-on RH ... modules. ...

2001-02-01

383

Identification of optimum sites for power system stabilizer applications

... dressing changes and site care, mid-line placement, placement of peripherally inserted central catheters, heparin locks, and patient education. ...

1993-08-01

384

INVESTIGATION OF THE CORROSION RESISTANCE OF BS ...

Many existing methods of identifying the optimum sites for installing power system stabilizers (PSSs) in multi-machine systems are restricted to the sequential PSS application, which considers the enhancement of damping of just one critical electromechanical mode at a time; the eigenstructure analysis of the open-loop system, which does not take the control matrix (i.e., the B matrix in the linearized model x = Ax + Bu for power systems) into consideration. This paper presents a method of identifying the optimum sites for installing power system stabilizers. The advantages of this method are: it can identify the optimum sites for installing PSSs so that several electrochemical modes are damped out simultaneously; it takes both the eigenstructure of the open-loop system and the control matrix into consideration.

1990-11-01

385

... 1. IE1O and IM'15 exposed to field corrosion tests at five sites for periods of 12 and 24 months confirm previous findings. ...

1953-12-01

386

Hydrobia schoutedeni

... River at Ango Ango (about 4 km from Matadi (probably upstream)), and the second at Chaundron de ... proposal were to be adopted the site at Matadi would be lost. No conservation measures in ...

387

Hydrobia plena

Hanford Waste Management Plan, 1987

... River at Ango Ango (4 km south of Matadi (upstream)), and the second on a small tributary ... proposal were to be adopted the site at Matadi would be lost. No conservation measures in place ...

388

Hanford Speakers Bureau - Hanford Site

The purpose of the Hanford Waste Management Plan (HWMP) is to provide an integrated plan for the safe storage, interim management, and disposal of existing waste sites and current and future waste streams at the Hanford Site. The emphasis of this plan is, however, on the disposal of Hanford Site waste. The plans presented in the HWMP are consistent with the preferred alternative which is based on consideration of comments received from the public and agencies on the draft Hanford Defense Waste Environmental Impact Statement (HDW-EIS). Low-level waste was not included in the draft HDW-EIS whereas it is included in this plan. The preferred alternative includes disposal of double-shell tank waste, retrievably stored and newly generated TRU waste, one pre-1970 TRU solid waste site near the Columbia River and encapsulated cesium and strontium waste.

1987-01-01

389

Gravity_on_Spinning_Cylinders - NASA Quest

to Cool Spots Hanford Fun Facts Classroom Projects Famous People of Hanford Counterintelligence Home Employee Responsibilities CI Information CI Resources Points of Contact...

2011-10-02

390

Golden coast on the Black Sea; Goldkueste am Schwarzen Meer

You can find a discussion of time travel and Tipler's cylinder at this site: http://www.star-names.freeserve.co.uk/travel.htm ...

391

Geothermal: Related Links

The most profitable terrain in Romania is already sold. Investors are now looking for new wind power sites. The contribution presents details. (orig.)

2007-06-15

392

Geothermal: Hot Documents Search

GEOTHERMAL TECHNOLOGIES LEGACY COLLECTION - Related Links Geothermal Technologies Legacy Collection Help/FAQ | Site Map | Contact Us | Admin Log On Home/Basic Search About...

2011-08-19

393

GEOTHERMAL TECHNOLOGIES LEGACY COLLECTION - Hot Documents Search Geothermal Technologies Legacy Collection Help/FAQ | Site Map | Contact Us | Admin Log On Home/Basic Search About...

2011-08-19

394

Geothermal: Contact Us

Foodstuff Concentrations and Relocation Considerations Following a Tritium Oxide Release from SRS Tritium Facilities

GEOTHERMAL TECHNOLOGIES LEGACY COLLECTION - Contact Us Geothermal Technologies Legacy Collection Help/FAQ | Site Map | Contact Us | Admin Log On Home/Basic Search About...

2011-08-19

395

Engineering assessment of inactive uranium mill tailings, Tuba City site, Tuba City, Arizona

The ingestion pathway consequences following an accidental tritium release from the Savannah River Site Tritium Facilities are evaluated.

1999-05-18

396

Energy Engineering Analysis Program (EEAP), limited energy study, Watervliet Arsenal, Watervliet, New York, site survey forms-ancillary facilities. Volume IIIA. Final report

Ford, Bacon and Davis Utah Inc. has reevaluated the Tuba City site in order to revise the March 1977 engineering assessment of the problems resulting from the existence of radioactive uranium mill tailings at Tuba City, Arizona. This engineering assessment has included the preparation of topographic maps, the performance of core drillings and radiometric measurements sufficient to determine areas and volumes of tailings and radiation exposures of individuals and nearby populations, the investigations of site hydrology and meteorology, and the evaluation and costing of alternative corrective actions. Radon gas released from the 0.8 million tons of tailings at the Tuba City site constitutes the most significant environmental impact, although windblown tailings and external gamma radiation also are factors.

1981-09-01

397

EPA - Stormwater Discharges from Construction Activities

No abstract available.

1992-04-01

398

Detecting hot spots at hazardous-waste sites

discharges. Proposed Stormwater ELG from Construction Sites - EPA has proposed effluent limitations guidelines (ELGs) and new source performance standards (NSPS) to control...

2011-10-14

399

Construction of the Savannah River Ecology Laboratory Conference Center

Evaluating the need for remedial cleanup at a waste site involves both finding the average contaminant concentration and identifying highly contaminated areas, or hot spots. A nomographic procedure to determine the sample configuration needed to locate a hot spot is presented. The technique can be used to develop a waste-site sampling plant - to determine either the grid spacing required to detect a hot spot at a given level of confidence, or the probability of finding a hot spot of a certain size, given a particular grid spacing. The method and computer program (ELIPGRID) were developed for locating geologic deposits, but the basic procedure can also be used to detect hot spots at chemical- or nuclear-waste disposal sites. Nomographs based on the original program are presented for three sampling-grid configurations - square, rectangular and triangular.

1984-07-09

400

Coastal Food Storage Locations- Kenai Fjords National Park

This Environmental Assessment (EA) reviews the environmental consequences associated with the proposed action of granting a site use permit to construct and operate a conference center on an approximately 70-acre tract of land on the Savannah River Site (SRS). While the proposed action requires an administrative decision by DOE, this EA reviews the linked action of physically constructing and operating a conference center. The SRS is a DOE-owned nuclear production facility encompassing approximately 200,000 acres in southwestern South Carolina. The proposed conference center would have an area of approximately 4,000 square feet, and would infrequently accommodate as many as 150 people, with the average being about 20 people per day. In addition to the No-Action alternative, under which the Research Foundation would not require the 70-acre tract of SRS land for a conference center, this EA considers site preservation. Under ...

2006-05-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

CIK (Cook Inlet/Kenai Peninsula) Marine Mammal Haul-Out Areas

This layer represents the different food storage facilities available at backcountry campsites along the coast of Kenai Fjords National Park. Site locations ... ...

402

Bioremediation of oil-contaminated soils: A recipe for success

... lion haul-out sites for the Cook Inlet Kenai Peninsula (CIK) region. This coverage was updated from ... ...

403

BAMONA's blog | Butterflies and Moths of North America

Bioremediation of land crude oil and lube oil spills is an effective and economical option. Other options include road spreading (where permitted), thermal desorption, and off-site disposal. The challenge for environment and operations managers is to select the best approach for each remediation site. Costs and liability for off-site disposal are ever increasing. Kerr-McGee`s extensive field research in eastern and western Texas provides the data to support bioremediation as a legitimate and valid option. Both practical and economical bioremediation as a legitimate and valid option. Both practical and economical, bioremediation also offers a lower risk of, for example, Superfund clean-up exposure than off-site disposal.

1995-12-31

404

Amchitka Mud Pit Sites 2006 Post-Closure Monitoring and Inspection Report, Amchitka Island, Alaska, Rev. No.: 0

site for reference information. Other recipients include Google's Translator, WikiLeaks, The ICUN Red List of Threatened Species, and the Public Library of Science (PLOS)....

2011-09-17

405

Aircraft Survivability: Protecting and Integrating Air and Space ...

In 2001, the U.S. Department of Energy (DOE), National Nuclear Security Administration (NNSA/NSO) remediated six areas associated with Amchitka mud pit release sites located on Amchitka Island, Alaska. This included the construction of seven closure caps. To ensure the integrity and effectiveness of remedial action, the mud pit sites are to be inspected every five years as part of DOE's long-term monitoring and surveillance program. In August of 2006, the closure caps were inspected in accordance with the ''Post-Closure Monitoring and Inspection Plan for Amchitka Island Mud Pit Release Sites'' (Rev. 0, November 2005). This post-closure monitoring report provides the 2006 cap inspection results.

2006-09-01

406

Addt'l. Earth Fill-McMurdo

... reliability of fiber optics in preference over space-based systems. ... STC web site at www.aiaa.org/tc ... and improve lethalities of US weapon systems 3 ...

2011-05-15

407

Adaptive Management of Liver Cancer Radiotherapy

... of this material is, however, outside the currently approved earth fill materials collection area ... fill material suitable for its intended use. The currently approved site contains material that is ...

408

A teleconferencing system.

Adaptive radiation therapy for liver cancer has the potential to reduce normal tissue complications and enable dose escalation, allowing the potential for tumor control in this challenging site....Full Text Available

2010-04-01

409

A randomised controlled trial of intervention site radiotherapy in malignant pleural mesothelioma

Training of health professionals commonly involves multiple sites in order to best utilize the available clinical material. However, this makes it difficult to achieve continuity in the presentation...Full Text Available

1993-01-01

410

Biosphere analyses for the safety assessment SR-Site - synthesis and summary of results

Background and purpose: To assess the effectiveness of radiotherapy in preventing tumour seeding after chest drain or pleural biopsy in patients with malignant mesothelioma and to determine, if tract metastases appear, whether they are tender or troublesome to patients. Patients and methods: Patients with a histological diagnosis of pleural mesothelioma and an invasive procedure within the preceding 21 days were stratified by age, performance status and treatment centre. Randomisation was performed between immediate drain site radiotherapy 21 Gy in three fractions (XRT arm) or best supportive care (BSC) with follow-up to 12 months. Patients were asked to complete questionnaires on treatment toxicity and on symptoms from any tract metastases detected. Results: Sixty-one patients were recruited from two centres between 1998 and 2004; 56 men, 5 women, median age 70. 31 were allocated to drain site radiotherapy. Seven patients developed tract ...

2007-07-01

411

The radioecological risk of decommissioning of nuclear submarines. Possible accidents and normal conditions

This report summarises nearly 20 biosphere reports and gives a synthesis of the work performed within the SR-Site Biosphere project, i.e. the biosphere part of SR-Site. SR-Site Biosphere provides the main project with dose conversion factors (LDFs), given a unit release rate, for calculation of human doses under different release scenarios, and assesses if a potential release from the repository would have detrimental effects on the environment. The intention of this report is to give sufficient details for an overview of methods, results and major conclusions, with references to the biosphere reports where methods, data and results are presented and discussed in detail. The philosophy of the biosphere assessment was to make estimations of the radiological risk for humans and the environment as realistic as possible, based on the knowledge of present-day conditions at Forsmark and the past and expected future development of ...

2010-12-15

412

In the report the results of the estimations of radiological risk of various stages of decommissioning of nuclear submarines are presented. At occurrence on nuclear submarine the heavy failure, relating to the class hypotetical volume of acting of radionuclides in atmosphere can reach 1.6E(15) Bq. Results of estimations probable doses on an axis of a trace of a radioactive loop show, that at distribution of radionuclides during atmospheric carry to 'agreed' settlement (500-1000 m) the maximum doses on its territory can make: about 6.0E(-3) Sv (for the whole body); 3.0E(-3) Gy for the leather (basal layer); 6.3E(-2) Gy for the lungs (acute exposure) and up to 1.8 Gy for the thyroid gland. Hypotetical failure for the estimation of the greatest possible radioecological consequences for hydrobiocenosis is considering, connected with single discharge of liquid radioactive waste (LRW) in water area. At navigating failure of the tanker with LRW in water area can arrive 300 M3 of LRW with the ...

2000-05-01

413

Volcano Field Trip

Uranium Mill Tailings Remedial Action Project (UMTRAP), Slick Rock, Colorado, Revision 1. Volume 1, Calculations, Final design for construction

This site focuses on the subject of volcanoes, including how volcanoes form, the destruction they can cause, effects of volcanoes on the environment, where active volcanoes are found, volcanoes of other planets, and the different volcano types. Designed as an electronic tour, it guides the user through different web sites and outlines teaching objectives, concepts covered, and new vocabulary. There is a teacher resources section with links for more information, and questions for students to answer along the way.

414

USE OF ENVIRONMENTAL ISOTOPES TO DIFFERENTIATE WATER SOURCES AND CONSTRAIN THE WATER BUDGET AT THE SULPHUR BANK MERCURY MINE, CLEAR LAKE, CALIFORNIA

Volume one contains calculations for: embankment design--embankment material properties; Union Carbide site--bedrock contours; vicinity properties--origin of contamination; North Continent and Union Carbide sites contaminated materials--excavation quantities; and demolition debris--quantity estimate.

1995-09-01

415

The Savannah River Site's Groundwater Monitoring Program - Fourth Quarter 1999 (October through December 1999)

The Sulphur Bank Mercury Mine (SBMM) is a 65 ha site located on the eastern shore of the Oaks Arm of Clear Lake, Lake County, California. Between 1864 and 1957, SBMM was the site of underground and open pit mining operations for S and Hg, coinciding with past and present hot spr...

416

Structural constraints for proposed Fort Hancock low-level radioactive waste disposal site (NTP-S34), southern Hudspeth County, Texas

This report summarizes the Groundwater Monitoring Program conducted by the Savannah River site during fourth quarter 1999. It includes the analytical data, field data, data review, quality control, and other documentation for this program; provides a record of the program's activities; and serves as an official records of the analytical results.

2000-10-12

417

Sandia National Laboratories/California site environmental report for 1997

Structural complexities reduce the homogeneity necessary for a site characterization model to an unacceptable level for performance assessment for radioactive waste disposal sites. The proposed site lies between the northern, stable Diablo platform and the southern, mobile Mesozoic Chihuahua tectonic belt. Structural movement along this interface has been active for the past 14,000 years. In addition, the area lies along the northern margin of the Permian Marfa basin and the northeastern margin of the deeply faulted Hueco bolson segment of the late Cenozoic Rio Grande rift system. Recent seismic activity with extensive surface rupture in Quitman Canyon (30 mi southeast of the site) is also documented from the 1931 Valentine, Texas, earthquake (6.4 Richter scale). The site is underlain by either a thrust fault or the complex terminus of a Mesozoic thrust fault. This fault is a ...

1989-03-01

418

Revegetation of inactive U-tailing sites

Sandia National Laboratories (SNL) is committed to conducting its operations in an environmentally safe and sound manner. It is mandatory that activities at SNL/California comply with all applicable environmental statutes, regulations, and standards. Moreover, SNL/California continuously strives to reduce risks to employees, the public, and the environment to the lowest levels reasonably possible. To help verify effective protection of public safety and preservation of the environment, SNL/California maintains an extensive, ongoing environmental monitoring program. This program monitors all significant effluents and the environment at the SNL/California site perimeter. Lawrence Livermore National Laboratory (LLNL) performs off-site external radiation monitoring for both sites. These monitoring efforts ensure that emission controls are effective in preventing contamination of the environment. As part of SNL/California`s ...

1998-06-01

419

Radisson community heating systems: technical and economic comparison of options. Final report

Soil placed over any sealant/barrier system can provide a protective mantle if the soil is not lost by erosion. Vegetation is an attractive choice for controlling erosion because it can provide an economical self-renewing cover that serves to reduce erosion by both wind and water. The objective of this research and development effort is to select and test vegetation strategies, including the choice of species and methods for revegetation that are compatible with sealant/barrier systems and are suited to soils and climates at inactive uranium mill tailings sites.

1981-02-01

420

Radionuclide air emissions report for the Hanford Site -- calendar year 1997

The study reported was conducted to evaluate water source community heat pump systems for possible installation to serve a number of homes which are to be constructed in the northeast section of Radisson, New York. Although specific sites and facilities have been evaluated, it is the intent of this study to be site specific only to the extent of climatic conditions. In other words, the results of this study should apply to any area with similar climate.

1981-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Polychlorinated Biphenyl Presence in the Columbia River Corridor

This report documents radionuclide air emission from the Hanford Site in 1997, and the resulting effective dose equivalent to the maximally exposed member of the public, referred to as the MEI. The report has been prepared in accordance with reporting requirements in the Code of Federal Regulations, Title 40, Protection of the Environment, Part 61, National Emissions Standards for Hazardous Air Pollutants, Subpart H, National Emission Standards for Emissions of Radionuclides Other than Radon from Department of Energy Facilities. This report has also been prepared in accordance with the reporting requirements of the Washington Administrative Code Chapter 246-247, Radiation Protection-Air Emissions. The effective dose equivalent to the MEI from the Hanford Site`s 1997 point source emissions was 1.2 E-03 mrem (1.2 E-05 mSv), which is well below the 40 CFR 61 Subpart H regulatory limit of 10 mrem/yr. Radon and thoron emissions, exempted from 40 CFR ...

1998-06-17

422

Localization of an anti-tumour monoclonal antibody in human tumour xenografts: kinetic and quantitative studies with the 791T/36 antibody

The U. S. Department of Energy (DOE) is required by Comprehensive Environmental Response, Compensation, and Liability Act of 1980 regulations to develop a conceptual understanding of potential contaminant releases from the Hanford Site based on an evaluation of existing data and known historical practices. Polychlorinated biphenyls (PCBs) are one environmental contaminant potentially released through leaks, spills, or disposal. This document presents a summary of selected relevant existing information, including environmental studies and Hanford Site analytical data.

2007-09-06

423

NAME=\\

Wastenet

...Project objectives to restore/create network of secure lapwing breeding sites to provide conservation management advice to planners, developers and land managers to raise awareness of lapwing conservation issues in the local areaKey dates so far First pair of lapwing breeding at Parc Bryn Bach, mitigation site for loss of a nearby large lapwing breeding colony, in May 2008Work planned or underway Continued working with developers and landowners to ...

424

Learn More About Networking

Studies have been performed with an anti-osteogenic sarcoma monoclonal antibody 791T/36 and human tumour xenografts to examine some of the quantitative and kinetic aspects of tumour localization. The influence of variations in tumour size and site and antibody dose on the extent and rate of specific antibody localization have been examined, together with the site of intratumoural deposition of the antibody. (UK).

425

LANL environmental restoration site ranking system: System description. Final report

These are some interesting sites that will help you to understand networking and how it can benefit you. These sites contain sound so you may want to wear headphones if you are in a classroom. Learn how the internet began and the basics of the www. Learn why a network is useful. Jans network contains the important concepts of networking, Work through section 7 to learn about different types of connections, transmissions, media, and configurations. Then take the quiz at the end to see how ...

2007-10-12

426

Hanford Site pollution prevention progress report

The basic structure of the LANL Environmental Restoration (ER) Site Ranking System and its use are described in this document. A related document, Instructions for Generating Inputs for the LANL ER Site Ranking System, contains detailed descriptions of the methods by which necessary inputs for the system will be generated. LANL has long recognized the need to provide a consistent basis for comparing the risks and other adverse consequences associated with the various waste problems at the Lab. The LANL ER Site Ranking System is being developed to help address this need. The specific purpose of the system is to help improve, defend, and explain prioritization decisions at the Potential Release Site (PRS) and Operable Unit (OU) level. The precise relationship of the Site Ranking System to the planning and overall budget processes is yet to be determined, as the system is still ...

1992-10-13

427

Hanford Internal Dosimetry Program Manual, PNL-MA-552

The Richland Operations Office (RL) and Office of River Protection (ORP) are pleased to issue the attached Pollution Prevention Progress Report. We have just met the most aggressive waste reduction and A recycling goals to date and are publishing this report to recognize A the site's progress, and to ensure it will sustain success beyond 1 Fiscal Year 2000. This report was designed to inform the been made by RL and ORP in Waste Minimization (WMin) and Pollution Prevention (P2). RL, ORP and their contractors are committed to protecting the environment, and we reiterate pollution prevention should continue to be at the forefront of the environmental cleanup and research efforts. As you read the attached report, we believe you will see a clear demonstration of RL and ORP's outstanding performance as it has been responsible and accountable to the nation, its employees, and the community in which we live and work. commitment that all employees have for ...

1999-10-05

428

Geothermal progress monitor. Progress report No. 7

This manual is a guide to the services provided by the Hanford Internal Dosimetry Program (IDP), which is operated by the Pacific Northwest National Laboratory.( ) for the U.S. Department of Energy Richland Operations Office, Office of River Protection and their Hanford Site contractors. The manual describes the roles of and relationships between the IDP and the radiation protection programs of the Hanford Site contractors. Recommendations and guidance are also provided for consideration in implementing bioassay monitoring and internal dosimetry elements of radiation protection programs.

2009-09-24

429

Federal Facilities Compliance Act, Draft Site Treatment Plan: Compliance Plan Volume. Part 2, Volume 2

A state-by-state review of major geothermal-development activities during 1982 is presented. It also inlcudes a summary of recent drilling and exploration efforts and the results of the 1982 leasing program. Two complementary sections feature an update of geothermal direct-use applications and a site-by-site summary of US geothermal electric-power development.

1983-04-01

430

Event Details - CECS - ANU

This document presents the details of the implementation of the Site Treatment Plan developed by Ames Laboratory in compliance with the Federal Facilities Compliance Act. Topics discussed in this document include: implementation of the plan; milestones; annual updates to the plan; inclusion of new waste streams; modifications of the plan; funding considerations; low-level mixed waste treatment plan and schedules; and TRU mixed waste streams.

1994-08-31

431

Wastenet

... Bob, who also teaches the Computer Networks and Security course in the Department, has some concerns when it comes to these large monolithic social-networking sites, such as Facebook and MySpace . He would prefer to see people using a more distributed (or federated) approach to avoid single sites being able to gather lots of personal information, including relationships between people . However, most ...

432

Estimation of air tritium concenration around Wolsung NPP site using a Lagrangian atmopsheric dispersion model

EFFECTS OF PH AND PHOSPHATE ON METAL DISTRIBUTION WITH EMPHASIS ON AS SPECIATION AND MOBILIZATION IN SOILS FROM A LEAD SMELTING SITE

A Lagrangian atmospheric dispersion model(K-LADM) combining a three dimensional sea-land breeze model has been developed and applied to the estimation of the quaterly and the annual averaged air tritium concentration around Wolsung NPP site. The estimated concentrations were compared with the observed concentration data. The results showed that the present Lagrangian Atmospheric dispersion model(K-LADM) provided very good agreement with the observations.

1998-10-01

433

Derivation of guidelines for uranium residual radioactive material in soil at the Colonie Site, Colonie, New York

Arsenic in soils from the Asarco Lead Smelter in East Helena, Montana was characterized by X-ray absorption spectroscopy (XAS). As oxidation state and geochemical speciation were analyzed as a function of depth (two sampling sites) and surface distribution. These results were c...

434

Residual radioactive material guidelines for uranium in soil were derived for the Colonie site located in Colonie, New York. This site has been designated for remedial action under the Formerly Utilized Sites Remedial Action Program (FUSRAP) of the U.S. Department of Energy (DOE). The site became contaminated with radioactive material as a result of operations conducted by National Lead (NL) Industries from 1958 to 1984; these activities included brass foundry operations, electroplating of metal products, machining of various components using depleted uranium, and limited work with small amounts of enriched uranium and thorium. The Colonie site comprises the former NL Industries property, now designated the Colonie Interim Storage Site (CISS), and 56 vicinity properties contaminated by fallout from airborne emissions; 53 of the vicinity properties were ...

1996-05-01

435

Closure of Off-Site FTP

CERN Multimedia

To reduce the number of regular break-ins on CERN machines due to passwords exposed on the network in clear text, OFF-SITE FTP ACCESS TO CERN WILL BE BLOCKED in the CERN firewall from: Tuesday 20th January 2004 If you use ftp to access CERN computers from outside CERN then please see the link below for alternative access means and further advice: http://cern.ch/security/ftp Denise Heagerty, CERN Computer Security officer, Computer.Security@cern.ch

2004-01-01

436

Closure of Off-Site FTP

Bone scintigraphy in Ewing's sarcoma during and after treatment - prognostic information from the primary tumor site

To reduce the number of regular break-ins on CERN machines due to passwords exposed on the network in clear text, OFF-SITE FTP ACCESS TO CERN WILL BE BLOCKED in the CERN firewall from: Tuesday 20th January 2004 If you use ftp to access CERN computers from outside CERN then please see the link below for alternative access means and further advice: http://cern.ch/security/ftp Denise Heagerty, CERN Computer Security officer, Computer.Security@cern.ch

2003-01-01

437

An immunochemical approach to the study of DNA damage and repair

A bone scan can be negative in Ewing's sarcoma. The bone scan during and after treatment can give prognostic information on the primary tumor site: A persisting hot spot strongly suggests the presence of local malignancy, while a hot spot becomming negative points to local cure of Ewing's sarcoma. (orig.).

438

Addendum to the 1996 Gunnison Monitoring Report for the Gunnison, Colorado Wetlands Mitigation Plan

The overall objective of this project is to produce antibodies to unique modified DNA bases and develop immunochemical assays to quantitate these lesions in damaged DNA. During this past year we have developed an antibody and chemical test to quantitate a basic sites in DNA and produced antibodies to the 8-oxopurines. This report discusses the detection of a basic sites in DNA and the preparation of antibodies to 8-hydroxyadenine and 8-hydroxyguanine.

1990-11-14

439

A New Metal-Binding Site for Yeast Phosphoglycerate Kinase as Determined by the Use of a Metal-ATP Analog

This document is an addendum to the 1996 Gunnison Monitoring Report for the Gunnison, Colorado, Wetlands Mitigation Report, dated July 1997. The purpose of this addendum is to: (1) modify how information on plant height and plant species criteria are presented; and (2) provide more detailed information regarding the evaluation of the bare ground criteria at the Camp Ketle site. The information in this addendum is provided at the request of the Bureau of Land Management to aid in future monitoring and evaluation of the wetland mitigation sites.

1997-10-01

440

Visualization of growth factor receptor sites in rat forebrain

Suicide substrate β, γ-bidentate Rh(III)ATP (RhATP) was used to map the metal ion-binding site in yeast phosphoglycerate kinase (PGK). Cleavage of the RhATP-inactivated enzyme with...Full Text Available

1997-02-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

Vascular plants of the Hanford Site

It is now known that various growth factors may also act in the central nervous system. Among them, it has recently been shown that epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) may possess trophic effects in the mammalian brain. We report here on the respective autoradiographic distribution of ["1"2"5I]EGF and ["1"2"5I]IGF-I receptor binding sites in the rat brain, both during ontogeny and in adulthood. It appears that ["1"2"5I]EGF sites are mostly found in the rat forebrain during brain development. On the other hand, ["1"2"5I]IGF-I sites are more widely distributed both during ontogeny and in adulthood. These results reveal the plasticity of the expression of EGF and IGF-I receptor sites in the mammalian brain. This could be relevant for the respective role of these two growth factors in the development and maintenance of neuronal function.

442

Transient diffusion, desorption, and reaction studies of cyclopropane and propylene with NaX and Eu/NaX zeolites

An updated listing of the vascular plants of the Hanford Site is provided, along with discussions of how humans may interact with local plants and have influenced the regional flora. Based on examinations of herbarium collections at the Westinghouse Hanford Company, Pacific Northwest Laboratory, Washington State University, and Brigham Young University, 590 vascular plant species have been identified on or near the Hanford Site. This is more than twice the number of species on previously published lists of Hanford Site vascular plants. A review of the plant species that are currently listed as endangered, threatened, sensitive, or otherwise of concern to the Washington State Natural Heritage Program and the Federal Fish and Wildlife Service is included. Color photographs of selected species are included to aid identification. Lists are provided of the Hanford Site plant species that could be used as ...

1992-07-01

443

Secondary reactions during CO hydrogenation on zeolite-supported metal catalysts: influence of alkali cations

The exchange of Eu[sup 3+] for Na[sup +] cations into the sodalite cages of X zeolite (Eu[sub 25]Na[sub 11]X) leads selectively to the isomerization reaction of cyclopropane to propylene. The latter reaction is catalyzed by Broensted acid sites with an apparent activation energy of 10.6 kcal/mol. Sorption measurements of cyclopropane and propylene with Eu/NaX and NaX zeolites at 40 C support the view that Na[sup +] cations might be considered as sites for sorption of these molecules. Force fields created by Eu[sub 4]O[sup 10+] present in Eu/NaX zeolite may affect sorption. On the other hand, Broensted acid sites in Eu/NaX enhance sorption of cyclopropane and propylene at 40 C. Chemisorption of propylene on the Broensted acid sites of Eu/NaX is reversible and may occur via a propylene carbenium cation intermediate. Small amounts of hexene are formed during this sorption. The amount of Broensted acid ...

1992-05-01

444

Reactions of 3-methylpentane and 2,3-dimethylbutane on aluminosilicate catalysts

The effect of neutralizing cations on the secondary reactions of the primary products from CO hydrogenation over ion-exchanged zeolite-supported Ru catalysts was investigated using zeolites with different alkali cations (Li/sup +/, Na/sup +/, K/sup +/, Rb/sup +/, Cs/sup +/). The transformation of olefins (propylene and butene) on the zeolites without the metal, under conditions similar to those used for CO hydrogenation, was also studied in order to understand the effect of the various constituents of the support, i.e., the Broensted acid sites generated during catalyst preparation and the alkali cations, on possible secondary reactions of the primary olefinic products. It was established that secondary acid-catalyzed reactions of these primary products can play a major role in shaping product selectivity during CO hydrogenation over zeolite-supported catalysts. Depending on the concentration and the strength of the acid sites, various ...

1987-10-01

445

Predicting Knot and Catenane Type of Products of Site-specific Recombination on Twist Knot Substrates

Catalytic reactions of 3-methylpentane and 2,3-dimethylbutane on HY, amorphous silica-alumina, and HZSM-5 have been studied at 500{degree}C. Both kinetic phenomena and product selectivities have been reported. Cracking reactions an HZSM-5 can be attributed to initiation through protonation occurring at Bronsted sites. Bimolecular processes leading to chain reaction via hydride transfer are restricted within the narrow pore pentasil zeolite. On HY and amorphous silica-alumina, initiation of cracking also occurs at Bronsted sited. No direct evidence was found for participation of Lewis acid sites on the catalyst framework itself. Following initiation, reactions on these catalysts are accelerated through a chain process occurring at Lewis sites generated by adsorption of product olefins at Bronsted sites. The resulting change in the dominant cracking mechanism is reflected in the ...

1990-12-01

446

Nevada Nuclear Waste Storage Investigations: Exploratory Shaft Facility fluids and materials evaluation

Site-specific recombination is an important cellular process that yields a variety of knotted and catenated DNA products on supercoiled circular DNA. Twist knots are some of the most common conformations of these products. They are also one of the simplest families of knots and catenanes. Yet, our systematic understanding of their implication in DNA and important cellular processes like site-specific recombination is very limited. Here we present a topological model of site-specific recombination characterising all possible products of site-specific recombination on twist knot substrates, extending previous work of Buck and Flapan. We illustrate how to use our model to examine previously uncharacterized experimental data. We show how our model can help determine the sequence of products in multiple rounds of processive recombination and distinguish between products of processive and distributive ...

2010-01-01

447

Near-surface velocity modeling at Yucca Mountain using borehole and surface records from underground nuclear explosions

The objective of this study was to determine if any fluids or materials used in the Exploratory Shaft Facility (ESF) of Yucca Mountain will make the mountain unsuitable for future construction of a nuclear waste repository. Yucca Mountain, an area on and adjacent to the Nevada Test Site in southern Nevada, USA, is a candidate site for permanent disposal of high-level radioactive waste from commercial nuclear power and defense nuclear activities. To properly characterize Yucca Mountain, it will be necessary to construct an underground test facility, in which in situ site characterization tests can be conducted. The candidate repository horizon at Yucca Mountain, however, could potentially be compromised by fluids and materials used in the site characterization tests. To minimize this possibility, Los Alamos National Laboratory was directed to evaluate the kinds of fluids and materials that will be used ...

1988-11-01

448

Methods and results of an evaluation of aquatic receptor risk at the Rocky Flats Environmental Technology Site, Golden, Colorado

The Department of Energy is investigating Yucca Mountain, Nevada as a potential site for commercial radioactive waste disposal in a mined geologic repository. One critical aspect of site suitability is the tectonic stability of the repository site. The levels of risk from both actual fault displacements in the repository block and ground shaking from nearby earthquakes are being examined. In particular, it is necessary to determine the expected level of ground shaking at the repository depth for large seismic sources such as nearby large earthquakes or underground nuclear explosions (UNEs). Earthquakes are expected to cause the largest ground motions at the site, however, only underground nuclear explosion data have been obtained at the repository depth level (about 350m below the ground level) to date. In this study we investigate ground motion from Nevada Test Site underground ...

2004-02-01

449

Localization of sites of photoaffinity labeling of the large subunit of Escherichia coli ribosomes by arylazide derivative of puromycin

The Rocky Flats Environmental Technology Site (RFETS) has historically released radionuclide chemicals of potential concern into the surrounding environment. The off-site environment was evaluated for Pu"2"3"9"/"2"4"0 and Am"2"4"1 occurrence. An evaluation of exposure and effects to the aquatic ecology within off-site areas including: Standley Lake, Great Western Reservoir, Mower Reservoir and portions of Big Dry Creek, Walnut Creek, and Woman Creek was performed for the completion of an Ecological Risk Assessment. Collocated sampling activities were performed for surface water, sediment, benthic macroinvertebrates and fish. Results of the analytical data were used to assess ongoing exposure and effects. Data collected to determine effects (chemical content of fish tissue, diversity and density of macroinvertebrate populations) provided some of the necessary information needed to evaluate risk. However, due to conditions of ...

1995-11-05

450

Localization of sites of photoaffinity labeling of the large subunit of Escherichia coli ribosomes by arylazide derivative of puromycin

Previous work showed that (TH)p-azidopuromycin photoaffinity labeled 70 S Escherichia coli ribosomes and that photoincorporation into 50 S subunit proteins was in the order L23 greater than L18/22 greater than L15. In the present work the authors report on immunoelectron microscopic studies of the complexes formed by p-azidopuromycin-modified 50 S subunits with antibodies to the N6,N6-dimethyladenosine moiety of the antibiotic. The p-azidopuromycin-modified 50 S subunits appear to be identical to unmodified control subunits in electron micrographs. Complexes of modified subunits with antibodies to the N6,N6-dimethyladenosine moiety of p-azidopuromycin were visualized in micrographs. Two regions of p-azidopuromycin photoincorporation were identified. The primary site, seen in about 75% of the complexes, is between the central protuberance and small projection, on the side away from the L7/L12 arm, in a region thought to contain the peptidyltransferase center. The ...

1985-08-25

451

Demonstration of Eastman Christensen horizontal drilling system -- Integrated Demonstration Site, Savannah River Site

Previous work showed that ["3H]p-azidopuromycin photoaffinity labeled 70 S Escherichia coli ribosomes and that photoincorporation into 50 S subunit proteins was in the order L23 greater than L18/22 greater than L15. In the present work the authors report on immunoelectron microscopic studies of the complexes formed by p-azidopuromycin-modified 50 S subunits with antibodies to the N6,N6-dimethyladenosine moiety of the antibiotic. The p-azidopuromycin-modified 50 S subunits appear to be identical to unmodified control subunits in electron micrographs. Complexes of modified subunits with antibodies to the N6,N6-dimethyladenosine moiety of p-azidopuromycin were visualized in micrographs. Two regions of p-azidopuromycin photoincorporation were identified. The primary site, seen in about 75% of the complexes, is between the central protuberance and small projection, on the side away from the L7/L12 arm, in a region thought to contain the peptidyltransferase center. The ...

452

Demonstration of Eastman Christensen horizontal drilling system -- Integrated Demonstration Site, Savannah River Site

An innovative horizontal drilling system was used to install two horizontal wells as part of an integrated demonstration project at the Savannah River Site (SRS), Aiken, South Carolina. The SRS is located in south-central South Carolina in the upper Coastal Plain physiographic province. The demonstration site is located near the A/M Area, and is currently known as the Integated Demonstration Site. The Department of Energy's Office of Technology Development initiated an integrated demonstration of innovative technologies for cleanup of volatile organic compounds (VOCS) in soils and groundwater at the SRS in 1989. The overall goal of the program is to demonstrate, at a single location, multiple technologies in the fields of drilling, characterization, monitoring, and remediation. Innovative technologies are compared to one another and to baseline technologies in terms of technical performance and cost effectiveness. ...

1992-12-01

453

Decision document for performing a long-term pumping test at the S-3 Site, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

An innovative horizontal drilling system was used to install two horizontal wells as part of an integrated demonstration project at the Savannah River Site (SRS), Aiken, South Carolina. The SRS is located in south-central South Carolina in the upper Coastal Plain physiographic province. The demonstration site is located near the A/M Area, and is currently known as the Integated Demonstration Site. The Department of Energy`s Office of Technology Development initiated an integrated demonstration of innovative technologies for cleanup of volatile organic compounds (VOCS) in soils and groundwater at the SRS in 1989. The overall goal of the program is to demonstrate, at a single location, multiple technologies in the fields of drilling, characterization, monitoring, and remediation. Innovative technologies are compared to one another and to baseline technologies in terms of technical performance and cost effectiveness. Transfer ...

1992-12-01

454

Constructing Folksonomies from User-specified Relations on Flickr

One of the principal problems confronting the remediation of Bear Creek Valley is the cleanup of contaminated groundwater. The S-3 Site is one of the locations in the valley where groundwater is most contaminated, and contamination from the S-3 Site has also caused extensive contamination of downgradient groundwater. This groundwater plume, therefore, has a high priority in the Bear Creek Valley remedial process. Pumping and treating groundwater was identified early in the feasibility study as a likely remedial alternative for the S-3 Site groundwater plume. The hydrology and geochemistry of the plume are extremely complex. There is a high degree of uncertainty in the current understanding of how the aquifer will react physically and chemically to pumping, making evaluation of a pump-and-treat alternative impractical at the present time. Before a pump-and-treat alternative can be evaluated, its technical practicability, ...

1995-02-01

455

Concentrations of radionuclides in terrestrial vegetation on the Hanford site of potential interest to Native Americans

Many social Web sites allow users to publish content and annotate with descriptive metadata. In addition to flat tags, some social Web sites have recently began to allow users to organize their content and metadata hierarchically. The social photosharing site Flickr, for example, allows users to group related photos in sets, and related sets in collections. The social bookmarking site Del.icio.us similarly lets users group related tags into bundles. Although the sites themselves don't impose any constraints on how these hierarchies are used, individuals generally use them to capture relationships between concepts, most commonly the broader/narrower relations. Collective annotation of content with hierarchical relations may lead to an emergent classification system, called a folksonomy. While some researchers have explored using tags as evidence for learning folksonomies, we believe ...

2008-01-01

456

Characterization and remediation of highly radioactive contaminated soil at Hanford

Concentrations of {sup 90}Sr and {sup 137}Cs in Carey`s balsamroot (Balsamorhiza careyana) and Gray`s desert parsley (Lomatium grayi) were similar to concentrations observed in other plants collected on the Hanford Site and from offsite locations surrounding the Site as part of annual Hanford Site surveillance. Observed concentrations may be attributed to historic fallout more than to Hanford Site emissions, although the observation that 200 Area plants had slightly higher concentrations of {sup 137}Cs than 100 Area plants is consistent with other monitoring data of radioactivity in soil and vegetation collected onsite. The present concentrations of {sup 90}Sr and {sup 137}Cs in balsamroot and parsley fluctuate around background levels with some of the higher observed concentrations of {sup 90}Sr found on the Fitzner/Eberhardt Arid Lands Ecology (ALE) Reserve. Analytical results and summary statistics ...

1995-03-01

457

Analysis of active site distribution in ZSM-5 crystals by infrared microscopy

The Hanford Site, Richland, Washington, contains over 1,500 identified waste sites and numerous groundwater plumes that will be characterized and remediated over the next 30 years. As a result of the Hanford Federal Facility Agreement and Consent Order, the US Department of Energy (DOE) has initiated a remedial investigation/feasibility study (RI/FS) at the 200-BP-1 operable unit. The 200-BP-1 RI/FS is the first Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) investigation on the Hanford Site that involves highly radioactive and chemically contaminated soils. The initial phase of site characterization was designed to assess the nature and extent of contamination associated with the source waste sites within the 200-BP-1 operable unit. Characterization activities consisted of drilling and sampling, chemical and physical analysis of samples, and ...

1993-09-01

458

An aerial radiological survey of the Hanford Site and surrounding area, Richland, Washington

The acid site distribution over large ZSM-5 crystals was analyzed using FTIR microscopy. Results of the analysis of the OH-stretching vibrations and of vibrations of pyridine adsorbed on Bronsted and Lewis acid sites were found to be in good agreement. The resolution which can be obtained using this novel technique is about 10-20 [mu]m. This limits the application to large crystals as analyzed in this study. Comparison of the results of IR microscopy with aluminum distributions obtained by electron microprobe analysis revealed that IR microscopy cannot resolve inhomogeneities on a scale smaller than the above-state limits. The results of both methods on a larger scale, however, are in very good agreement. Using a combination of both techniques, it is possible to obtain a clearer picture of the distribution of acid sites in large zeolite crystals, where electron microprobing gives the higher resolution, and IR microscopy ...

1993-10-01

459

Probing the Early Stages of Low-Mass Star Formation in LDN 1689N: Dust and Water in IRAS 16293-2422A, B, and E

An aerial radiological survey was conducted over the Department of Energy's Hanford Site near Richland, Washington, during the period 5 July through 26 August 1988. The survey was expanded, and additional flights were conducted to the east of the site and along the banks of the Columbia River down to McNary Dam near Umatilla. The survey was flown at altitude of 61 meters (200 feet) by a helicopter containing 17 liters (eight 2 in. x 4 in. x 16 in.) of sodium iodide detectors. Gamma ray data were collected over the survey area by flying north-south lines spaced 122 meters (400 feet) apart. The processed data indicated that detected radioisotopes and their associated gamma ray exposure rates were generally consistent with those expected from normal background emitters and man-made fission/activation products resulting from activities at the site. External exposure rates were generally 10 microroentgens per hour (#mu#R/h) with ...

1990-09-16

460

Preparation and characteristics of rice-straw-based porous carbons with high adsorption capacity

We present deep images of dust continuum emission at 450, 800, and 850 micron of the dark cloud LDN 1689N which harbors the low-mass young stellar objects (YSOs) IRAS 16293-2422A and B (I16293A and I16293B) and the cold prestellar object I16293E. Toward the positions of I16293A and E we also obtained spectra of CO-isotopomers and deep submillimeter observations of chemically related molecules with high critical densities. To I16293A we report the detection of the HDO 1_01 - 0_00 and H2O 1_10 - 1_01 ground-state transitions as broad self-reversed emission profiles with narrow absorption, and a tentative detection of H2D+ 1_10 - 1_11. To I16293E we detect weak emission of subthermally excited HDO 1_01 - 0_00. Based on this set of submillimeter continuum and line data we model the envelopes around I16293A and E. The density and velocity structure of I16293A is fit by an inside-out collapse model, yielding a sound speed of a=0.7 km/s, an age of ...

2004-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Luminous HC3N line emission in NGC4418 - buried AGN or nascent starburst?

To prepare porous carbons with high adsorption capacity from rice straws, two different kinds of precursors, i.e. one as the raw rice straws (one-stage process) and the other as pre-carbonized rice straws (two-stage process), were active with KOH of various impregnation ratios. The two-stage process was found very effective for manufacturing porous carbons with high surface area and adsorption capacities for MB and I{sub 2}. For example, the porous carbon that was carbonized at 700{sup o}C and subsequently activated at 900{sup o}C exhibited the surface area of 2410 m{sup 2}/g, the adsorption capacities of 800 and 1720 mg/g for MB and I{sub 2}, respectively, and the total pore volume of 1.4 ml/g. In the two-stage method, there was a preferential optimum impregnation ratio of KOH to a precursor carbon, i.e. 4:1, with which high surface area of porous carbons could be achieved. The formation of uni- and bidentate carboxylic salt structure, induced ...

2002-02-01

462

Isothermal heat measurements of TBP-nitric acid solutions

IRAM 30m observations reveal that the deeply obscured IR-luminous galaxy NGC4418 has a rich molecular chemistry - including unusually luminous HC3N line emission. We furthermore detect: ortho-H2CO 2-1, 3-2; CN 1-0, 2-1; HCO+, 1-0. 3-2, HCN 3-2, HNC 1-0, 3-2 (and tentatively OCS 12-11). The HCN, HCO+, H2CO and CN line emission can be fitted to densities of n=5 x 10E4 - 10E5 cm-3 and gas temperatures Tk=80-150 K. Both HNC and HC3N are, however, significantly more excited than the other species which requires higher gas densities - or radiative excitation through e.g. mid-IR pumping. The HCN line intensity is fainter than that of HCO+ and HNC for the 3-2 transition, in contrast to previous findings for the 1-0 lines where the HCN emission is the most luminous. We tentatively suggest that the observed molecular line emission is consistent with a young starburst, where the emission can be understood as emerging from dense, warm gas with an ...

2007-01-01

463

Vacancy ordering and oxygen dynamics in oxide ion conducting La1-xSrxGa1-xMgxO3-x ceramics: 71Ga, 25Mg and 17O NMR

Net heats of reaction were measured in an isothermal calorimeter for both single phase (organic) and two phase (organic and aqueous) TBP/HNO{sub 3} reacting solutions at temperatures above 100 C. The oxidation rate constant was determined to be 5.4E-4 min{sup {minus}1} at 110 C for an open ``vented`` system as compared to 1.33 E-3 min{sup {minus}1} in the closed system. The heat released per unit material oxidized was also reduced. The oxidation in both phases was found to be first order in nitric acid and pseudo-zero order in butylnitrate and water. The hydrolysis (esterification) rate constant determined by Nichols` (1.33E-3 min{sup {minus}1}) fit the experimental data from this work well. Forced evaporation of the volatile components by the product gases from oxidation resulted in a cooling mechanism which more than balanced the heat from the oxidation reaction in the two-phased systems. Rate expressions were derived and rate constants ...

1994-12-16

464

Spent Fuel Background Report Volume I

The oxygen vacancies distribution in the rigid lattice and the thermally activated motion of oxygen atoms are studied in La1-xSrxGa1-xMgxO3-x (x=0.00; 0.05; 0.10; 0.15 and 0.20) compounds. For that 71Ga, 25Mg and 17O NMR was performed from 100 K up to 670 K, and ion conductivity measurements were carried out up to 1273 K. The comparison of the electric field gradients at the Ga- and Mg-sites evidences that oxygen vacancies appear exclusively near gallium cations as a species trapped below room temperature in local clusters, GaO5/2-#square#-GaO5/2. These clusters decay at higher temperature into mobile constituents of the structural octahedra Ga(O5/6#square#1/6)6/2. At the same time, the nearest octahedral oxygen environment of magnesium cations persists at different doping levels. The case of two adjacent vacant anion sites is found highly unlikely within the studied doping range. The thermally activated oxygen motion starts to develop above ...

2011-01-01

465

Responses to comments on the remedial investigation/feasibility study-environmental impact statement for remedial action at the Chemical Plant area of the Weldon Spring site (November 1992)

This report is an overview of current spent nuclear fuel management in the DOE complex. Sources of information include published literature, internal DOE documents, interviews with site personnel, and information provided by individual sites. Much of the specific information on facilities and fuels was provided by the DOE sites in response to the questionnaire for data for spent fuels and facilities data bases. This information is as accurate as is currently available, but is subject to revision pending results of further data calls. Spent fuel is broadly classified into three categories: (a) production fuels, (b) special fuels, and (c) naval fuels. Production fuels, comprising about 80% of the total inventory, are those used at Hanford and Savannah River to produce nuclear materials for defense. Special fuels are those used in a wide variety of research, development, and testing activities. Special fuels include fuel from ...

1994-03-01

466

Ocean FUSRAP: feasibility of ocean disposal of materials from the Formerly Utilized Sites Remedial Action Progam (FUSRAP)

The US Department of Energy (DOE) is responsible for cleanup activities at the Weldon Spring site in St. Charles County, Missouri. The site consists of a chemical plant area and a noncontiguous limestone quarry; both areas are radioactively and chemically contaminated as a result of past processing and disposal activities. Explosives were produced by the US Army at the chemical plant in the 1940s, and uranium and thorium materials were processed by DOE`s predecessor agency in the 1950s and 1960s. During that time, various wastes were disposed of at both areas of the site. The DOE is conducting cleanup activities at the site under its Environmental Restoration and Waste Management Program. The integrated remedial investigation/feasibility study-environmental impact statement (RI/FS-EIS) documents for the chemical plant area were issued to the public in November 1992 as the draft RI/FS-EIS. (The CERCLA ...

1993-06-01

467

Ocean FUSRAP: feasibility of ocean disposal of materials from the Formerly Utilized Sites Remedial Action Progam (FUSRAP)

The Formerly Utilized Sites Remedial Action Program (FUSRAP) of the Department of Energy is designed to identify and evaluate the radiological conditions at sites formerly used by the Corps of Engineers Manhattan Engineer District and the US Atomic Energy Commission. Where required, remedial action will be instituted to remove potential restrictions on the use of the sites due to residual low-level radioactive contamination. A total of 31 sites that may require remedial action has been identified. The purpose of the Ocean FUSRAP Program, which began in March 1981, is to assess the technical, environmental, and institutional feasibility of disposing, in the ocean and on the ocean floor, of FUSRAP soil and rubble which contains traces of natural radioactive materials. The initial focus has been on the Middlesex, New Jersey, Sampling Plant site and surrounding properties, which contain ...

1982-01-01

468

Korea-Japan Joint Research on Development of Seismic Capacity Evaluation and Enhancement Technology Considering Near-Fault Effect

The Formerly Utilized Sites Remedial Action Program (FUSRAP) of the Department of Energy is designed to identify and evaluate the radiological conditions at sites formerly used by the Corps of Engineers Manhattan Engineer District and the US Atomic Energy Commission. Where required, remedial action will be instituted to remove potential restrictions on the use of the sites due to residual low-level radioactive contamination. A total of 31 sites that may require remedial action has been identified. The purpose of the Ocean FUSRAP Program, which began in March 1981, is to assess the technical, environmental, and institutional feasibility of disposing, in the ocean and on the ocean floor, of FUSRAP soil and rubble which contains traces of natural radioactive materials. The initial focus has been on the Middlesex, New Jersey, Sampling Plant site and surrounding properties, which contain ...

469

Final report for SNL/NM environmental drilling project

Several recent improved methods for the EGFM are introduced in order to avoid artificial holes seen in the synthetic acceleration spectrum. Furthermore evaluation of input ground motions at Wolsung NPP are performed by varying the source parameters that may control the high-frequency wave radiation and the deviation of the synthetic motions are revealed. The PSHA case studies for four NPP sites (Wolsung, Kori, Uljin, Younggwang) are performed. In the analysis, site-specific attenuation equations developed for Korean NPP sites are employed, and the seismic hazards for the target sites are evaluated in the case where the four kind of seismic source models are considered. Moreover, the PSHA for Wolsung and Younggwang are conducted by using the site-specific attenuation equation with the index of response spectra and the uniform hazard spectra are evaluated for the two ...

2005-12-15

470

Evaluation of the suitability of the WIPP site

Concern for the environment and cost reduction are driving forces for a broad effort in government and the private sector to develop new, more cost-effective technologies for characterizing, monitoring and remediating environmental sites. Secondary goals of the characterization, monitoring and remediation (CMR) activity are: minimize secondary waste generation, minimize site impact, protect water tables, and develop methods/strategies to apply new technologies. The Sandia National Laboratories (SNL) project in directional boring for CMR of waste sites with enhanced machinery from the underground utility installation industry was initiated in 1990. The project has tested a variety of prototype machinery and hardware built by the industrial partner, Charles Machine Works (CMW), and SNL at several sites (Savannah River Site (SRS), Hanford, SNL, Kirtland AFB (KAFB), CMW), successfully ...

1994-11-01

471

Computational identification of developmental enhancers:conservation and function of transcription factor binding-site clustersin drosophila melanogaster and drosophila psedoobscura

Determination of the suitability of the site for WIPP is only the first major phase in the evaluation of the radiological impact of the repository on the public health and safety. The Environmental Evaluation Group (EEG) will continue to independently review the design of the facility, the operational procedures, the criteria for packaging and shipment of the waste, the plans, procedures and results of the WIPP experiments, emergency preparedness, adherence to EPA and pertinent NRC regulations, and other important features of the project. EEG has concluded from existing evidence that the Los Medanos site for the WIPP project has been characterized in sufficient detail to warrant confidence in the validation of the site for the permanent emplacement of approximately 6 million cubic feet of defense transuranic waste. This conclusion is based on the assumption that the maximum surface dose rate for the unshielded ...

1983-05-01

472

Comparison of angiotensin II and SAR1, Ile8-AII binding to solubilized receptors in the bovine adrenal

Background The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. Results We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated to those of neighboring genes; the ...

2004-08-06

473

Arid sites stakeholder participation in evaluating innovative technologies: VOC-Arid Site Integrated Demonstration

Derivatives of angiotensin with a sarcosine substitution at the carboxy terminus and an aliphatic amino acid substitution at the amino terminus have been shown to be competitive ligands for the angiotensin receptor. Scatchard plots derived from saturation isotherm data in bovine adrenal particulates consistently demonstrate three to five times the number of binding sites for "1"2"5I-Sarl,Ile8-AII ("1"2"5I-SI-AII) than for "1"2"5I-AII in experiments in which the two ligands are run side-by-side. A significant difference between "1"2"5I-AII and "1"2"5I-SI-AII binding to detergent solubilized angiotensin receptors was observed. Of the "1"2"5I-AII binding sites, about 85-90% were recovered upon solubilization. In contrast, the recovery of "1"2"5I-SI-AII binding sites was only 4-5%. To determine whether binding sites labeled in the soluble state represent biologically significant AII receptors, they examined ...

1986-04-13

474

A simulation of the transport and fate of radon-222 derived from thorium-230 low-level waste in the near-surface zone of the Radioactive Waste Management Site in Area 5 of the Nevada Test Site

Developing and deploying innovative environmental cleanup technologies is an important goal for the U.S. Department of Energy (DOE), which faces challenging remediation problems at contaminated sites throughout the United States. Achieving meaningful, constructive stakeholder involvement in cleanup programs, with the aim of ultimate acceptance of remediation decisions, is critical to meeting those challenges. DOE`s Office of Technology Development sponsors research and demonstration of new technologies, including, in the past, the Volatile Organic Compounds Arid Site Integrated Demonstration (VOC-Arid ID), hosted at the Hanford Site in Washington State. The purpose of the VOC-Arid ID has been to develop and demonstrate new technologies for remediating carbon tetrachloride and other VOC contamination in soils and ground water. In October 1994 the VOC-Arid ID became a part of the Contaminant Plume Containment and Remediation ...

1995-05-01

475

2003 Data Report: Groundwater Monitoring Program, Area 5 Radioactive Waste Management Site, Nevada Test Site

US Department of Energy (DOE) Order 5820.2A (DOE, 1988) requires performance assessments on all new and existing low-level radioactive waste (LLW) disposal sites. An integral part of performance assessment is estimating the fluxes of radioactive gases such as radon-220 and radon-222. Data needs pointed out by mathematical models drive site characterization. They provide a logical means of performing the required flux estimations. Thorium-230 waste, consisting largely of thorium hydroxide and thorium oxides, has been approved for disposal in shallow trenches and pits at the LLW Radioactive Waste Management Site in Area 5 of the Nevada Test Site. A sophisticated gas transport model, CASCADR8 (Lindstrom et al., 1992b), was used to simulate the transport and fate of radon-222 from its source of origin, nine feet below a closure cap of native soil, through the dry alluvial earth, to its point of release into ...

1993-12-01

476

1993 Annual report on waste generation and waste minimization progress as required by DOE Order 5400.1, Hanford Site

This report is a compilation of the calendar year 2003 groundwater sampling results from the Area 5 Radioactive Waste Management Site, Nevada Test Site. Wells Ue5PW-1, Ue5PW-2, and Ue5PW-3 were sampled semi-annually for the required analytes: pH, specific conductance, total organic carbon (TOC), total organic halides (TOX), tritium, and major cations/anions. Results from all samples collected in 2003 were within established criteria. These data indicate that there has been no measurable impact to the uppermost aquifer from the Resource Conservation and Recovery Act (RCRA) regulated unit within the Area 5 Radioactive Waste Management Site and confirm that any previous detections of TOC and TOX were false positives. Contamination indicator data are presented in control chart and tabular form with investigation levels indicated. Gross water chemistry data are presented in graphical and tabular form. There were no major changes ...

2004-02-01

477

Hyperspectral and Multispectral Remote Sensing at Uranium Processing Facilities

More important than waste generation numbers, the pollution prevention and waste minimization successes achieved at Hanford in 1993 have reduced waste and improved operations at the Site. Just a few of these projects are: A small research nuclear reactor, unused and destined for disposal as low level radioactive waste, was provided to a Texas University for their nuclear research program, avoiding 25 cubic meters of waste and saving $116,000. By changing the slope on a asphalt lot in front of a waste storage pad, run-off rainwater was prevented from becoming mixed low level waste water, preventing 40 cubic meters of waste and saving $750,000. Through more efficient electrostatic paint spraying equipment and a solvent recovery system, a paint shop reduced hazardous waste by 3,500 kilograms, saving $90,800. During the demolition of a large decommissioned building, more than 90% of the building's material was recycled by crushing the concrete for use ...

478

Corrective Action Investigation Plan for Corrective Action Unit 409: Other Waste Sites, Tonopah Test Range, Nevada (Rev. 0)

Many mines and processing facilities are in remote, difficult to access areas, or are in areas where access is limited or restricted for national reasons. In a joint project with Canada Centre for Remote Sensing, the Canadian Safeguards Support Program is investigating utility of multi- and hyperspectral remote sensing for remotely inspecting such site. With a view to eventually using the hyperspectral satellite data now coming available, airborne data over uranium and copper mine tailings and uranium processing facilities in northern Canada were acquired. The objective of this work is to demonstrate that multispectral and hyperspectral data can provide complementary and supplementary information to high-resolution panchromatic imagery for the following safeguards applications: Inspection aids (up-to-date maps of remote locations), change detection, evaluation of member state declarations, monitoring of reactor, mining and processing facility operations, and ...

2003-05-01

479

Waste reduction at the Savannah River Site

This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 409 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 409 consists of three Corrective Action Sites (CASs): TA-53-001-TAB2, Septic Sludge Disposal Pit No.1; TA-53-002-TAB2, Septic Sludge Disposal Pit No.2; and RG-24-001-RGCR, Battery Dump Site. The Septic Sludge Disposal Pits are located near Bunker Two, close to Area 3, on the Tonopah Test Range. The Battery Dump Site is located at the abandoned Cactus Repeater Station on Cactus Peak. The Cactus Repeater Station was a remote, battery-powered, signal repeater station. The two Septic Sludge Disposal Pits were suspected to be used through the late 1980s as disposal sites ...

2000-10-05

480

Technical evaluation report for the demonstration of radio frequency soil decontamination at Site S-1

The Savannah River Site (SRS) is a key installation for the production and research of nuclear materials for national defense and peace time applications and has been operating a full nuclear fuel cycle since the early 1950s. Wastes generated include high level radioactive, transuranic, low level radioactive, hazardous, mixed, sanitary, and aqueous wastes. Much progress has been made during the last several years to reduce these wastes including management systems, characterization, and technology programs. The reduction of wastes generated and the proper handling of the wastes have always been a part of the Site's operation. This paper summarizes the current status and future plans with respect to waste reduction to waste reduction and reviews some specific examples of successful activities.

1990-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

The Air Force`s Armstrong Laboratory at Tyndall Air Force Base, Florida, has supported the research and development of Radio Frequency Soil Decontamination. Radio frequency soil decontamination is essentially a heat-assisted soil vapor extraction process. Site S-1 at Kelly Air Force Base, San Antonio, Texas, was selected for the demonstration of two patented techniques. The site is a former sump that collected spills and surface run-off from a waste petroleum, oils, and lubricants and solvent storage and transfer area. In 1993, a technique developed by the IIT Research Institute using an array of electrodes placed in the soil was demonstrated. In 1994, a technique developed by KAI Technologies, Inc. using a single applicator placed in a vertical borehole was demonstrated. Approximately 120 tons of soil were heated during each demonstration to a temperature of about 150 degrees Celsius.

1995-04-01

482

Site Operator Program

Collectively, the organizations participating in the Site Operator Program have over forty years of EV experience and have operated electric vehicles (EVs) for over 600,000 miles, providing the most extensive EV operating and knowledge base in the country. The Site Operator Program is intended to provide financial and technical support and organizational resources to organizations active in the advancement of electric vehicles. Support is provided for the demonstration of vehicles and the test and evaluation of vehicles, components, and batteries. Support is also provided for the management and support of the program for the participating organizations. The Program provides a forum for participants to exchange information among the group, as well as with vehicle and equipment manufacturers and suppliers, and the public. A central data base at the Idaho National Engineering Laboratory provides a repository for-data on the vehicles being operated ...

1991-01-01

483

Site Operator Program

Relationship between the lability of sediment-bound metals (Cd, Cu, Zn) and their bioaccumulation in benthic invertebrates

Collectively, the organizations participating in the Site Operator Program have over forty years of EV experience and have operated electric vehicles (EVs) for over 600,000 miles, providing the most extensive EV operating and knowledge base in the country. The Site Operator Program is intended to provide financial and technical support and organizational resources to organizations active in the advancement of electric vehicles. Support is provided for the demonstration of vehicles and the test and evaluation of vehicles, components, and batteries. Support is also provided for the management and support of the program for the participating organizations. The Program provides a forum for participants to exchange information among the group, as well as with vehicle and equipment manufacturers and suppliers, and the public. A central data base at the Idaho National Engineering Laboratory provides a repository for-data on the vehicles being operated ...

1991-12-31

484

British Library Electronic Table of Contents (United Kingdom)

The present study has investigated metal contamination at nine sites (10 sampling stations) from the English Channel to the Mediterranean Sea, including low level and highly contaminated sediments. Both total and labile concentrations of metals were determined in superficial sediments. The influence of different pHs was tested and metal lability at pHs encountered in the gut of invertebrates (the ragworm Nereis diversicolor, the blue mussel Mytilus edulis, the Japanese oyster Crassostrea gigas) was compared with the distribution of metals in various operationally defined geochemical fractions. Cd showed the highest lability and Cu the lowest, whereas Zn lability was intermediate. Metal concentrations were determined in bivalves at six sites and in worms at three sites. Cd in living organis...

2007-01-01

485

Presence of pathogenic microorganisms in power-plant cooling waters. Report for October 1, 1979-September 30, 1981

Preliminary seismic safety evaluation of the Uljin nuclear power plant site regarding the offshore Uljin earthquake on the 29 May 2004 as an empirical Green's function

Cooling waters from eleven geographically disparate power plants were tested for the presence of Naegleria fowleri and Legionella pneumophila (LDB). Control source waters for each plant were also tested for these pathogens. Water from two of the eleven plants contained pathogenic Naegleria, and infectious Legionella were found in seven of the test sites. Pathogenic Naegleria were not found in control waters, but infectious Legionella were found in five of the eleven control source water sites. Concentrations of nitrite, sulfate, and total organic carbon correlated with the concentrations of LDB. A new species of Legionella was isolated from one of the test sites. In laboratory tests, both Acanthamoeba and Naegleria were capable of supporting the growth of Legionella pneumophila.

1982-10-01

486

Pinellas Plant Environmental Baseline Report

The moderate earthquake of magnitude 5.2 was occurred at the offshore Uljin on the 29 May 2004. The magnitude of the event is the largest one which is equal to that of the Sokrisan earthquake on the 16 September 1978 since the beginning of the instrumental recording by the Korean Metrological Administration (KMA) in 1978. The magnitude of the event was large enough to be felt in a wide area of the southern Korea. It did not affect the safety of the Uljin nuclear power plant (NPP) site which is about 80 km away from the epicenter. In this article, we estimate source parameters of the event and evaluate preliminary seismic safety of the Uljin NPP site regarding the event as an empirical Green's function (EGF)

2010-10-01

487

Metallointercalators and Metalloinsertors

The Pinellas Plant has been part of the Department of Energy`s (DOE) nuclear weapons complex since the plant opened in 1957. In March 1995, the DOE sold the Pinellas Plant to the Pinellas County Industry Council (PCIC). DOE has leased back a large portion of the plant site to facilitate transition to alternate use and safe shutdown. The current mission is to achieve a safe transition of the facility from defense production and prepare the site for alternative uses as a community resource for economic development. Toward that effort, the Pinellas Plant Environmental Baseline Report (EBR) discusses the current and past environmental conditions of the plant site. Information for the EBR is obtained from plant records. Historical process and chemical usage information for each area is reviewed during area characterizations.

1997-06-01

488

Metallo-intercalators and metallo-insertors.

Since the elucidation of the structure of double helical DNA, the construction of small molecules that recognize and react at specific DNA sites has been an area of considerable interest. In particular, the study of transition metal complexes that bind DNA with specificity has been a burgeoning field. This growth has been due in large part to the useful properties of metal complexes, which possess a wide array of photophysical properties and allow for the modular assembly of an ensemble of recognition elements. Here we review recent experiments in our laboratory aimed at the design and study of octahedral metal complexes that bind DNA non-covalently and target reactions to specific sites. Emphasis is placed both on the variety of methods employed to confer site-specificity and upon the many applications for these complexes. Particular attention is given to the family of complexes recently designed that target single base ...

2007-09-20

489

Management of petroleum underground storage tanks at the Hanford Site

Since the elucidation of the structure of double helical DNA, the construction of small molecules that recognize and react at specific DNA sites has been an area of considerable interest. In particular, the study of transition metal complexes that bind DNA with specificity has been a burgeoning field. This growth has been due in large part to the useful properties of metal complexes, which possess a wide array of photophysical attributes and allow for the modular assembly of an ensemble of recognition elements. Here we review recent experiments in our laboratory aimed at the design and study of octahedral metal complexes that bind DNA non-covalently and target reactions to specific sites. Emphasis is placed both on the variety of methods employed to confer site-specificity and upon the many applications for these complexes. Particular attention is given to the family of complexes recently designed that target single base ...

2007-09-20

490

Hystersisters Online: Social Support and Social Comparison Among Hysterectomy Patients on the Internet

This report represents the timetables, responsible organizations, and methods required to comply with the newly promulgated Washington Administrative Code (WAC) 173-360 Underground Storage Tank (UST) Regulations which became effective December 29, 1990. This report only addresses UST systems that contain nonradioactive material. A total of 84 tanks at the Hanford Site are currently regulated under WAC 173-360. In addition, 32 regulated tanks have been removed as a result of the federally mandated program and the newly implemented state regulations. The majority of the USTs at the Hanford Site are operated by Westinghouse Hanford; however, one is operated by Kaiser Engineers Hanford (KEH) and one by Pacific Northwest Laboratory (PNL).

1991-09-08

491

British Library Electronic Table of Contents (United Kingdom)

Background: The Internet has become a popular source of health information for patients with a variety of medical concerns; however, research examining patient interactions on the Internet has been limited. Purpose: Four questions were examined in a survey study of hysterectomy patients who visited http://www.hystersisters.com: (a) Do hysterectomy patients use the support Web site because they perceive their proximal sources of social support to be inadequate? (b) What kinds of support do patients receive from the Web site? (c) What attributes characterize the "Hystersisters" that are perceived to be most helpful? (d) How do informational sources vary depending on the nature of the patient concern? Methods: Women (N = 137) responded to questions about social support, Web site use, and perc...

2006-01-01

492

Geology, physical properties, and surface effects at Discus Thrower Site, Yucca Flat, Nevada Test Site

Final ROI Report - Technology Transfer of Waste-Reducing Groundwater Sampling Systems

Geologic studies in connection with Project Discus Thrower have furnished detailed stratigraphic and structural information about northwestern Yucca Flat, Nevada Test Site. The Paleozoic rocks consist of a lower carbonate sequence, argillite of the Eleana Formation, and an upper carbonate sequence. The distribution of these rocks suggests that both top and bottom of the Eleana are structural contacts, probably thrusts or reverse faults. The overlying tuff includes several units recognized in the subsurface, such as the Fraction Tuff and tuff of Redrock Valley. Other units recognized include bedded tuff associated with the Grouse Canyon Member of Belted Range Tuff, and the Rainier Mesa and Ammonia Tanks Members of the Timber Mountain Tuff. The Timber Mountain and Grouse Canyon are extensively altered to montmorillonite (a swelling clay), possibly as a result of ponding of alkaline water. The overlying alluvium locally contains at the base a clayey, tuffaceous ...

493

Environmental Survey preliminary report, National Institute for Petroleum and Energy Research, Bartlesville, Oklahoma

This report presents the findings of a U.S. DOE Environmental Management technology transfer initiative of waste-reducing ground water sampling systems between Savannah River Site (SRS) and Lawrence Livermore National Laboratory (LLNL) which occurred during fiscal years 2001 and 2002. The report describes the collaboration between the two sites, the deployment of the Savannah River Site Purge Water Management System at LLNL, the changes made to that system for use at LLNL, and documents the return-on-investment derived from the system's use at LLNL as well as other benefits generated through this inter-laboratory collaboration. An evaluation of the deployment of the LLNL EasyPump sampling technology at SRS will be covered in a separate report from SRS.

2002-09-30

494

Energy threat to valuable land

This report presents the preliminary findings of the first phase of the Environmental Survey of the US Department of Energy's (DOE) National Institute for Petroleum and Energy Research (NIPER), conducted February 29 through March 4, 1988. The Survey is being conducted by an interdisciplinary team of environmental specialists, led and managed by the Office of Environment, Safety and Health's Office of Environmental Audit. Team members are being provided by private contractors. The objective of the Survey is to identify environmental problems and areas of environmental risk associated with NIPER. The Survey covers all environmental media and all areas of environmental regulation. It is being performed in accordance with the DOE Environmental Survey Manual. The on-site phase of the Survey involves the review of existing site environmental data, observations of the operations carried on at NIPER and interviews with ...

1989-01-01

495

Development of anion-exchange resins for separations of actinides

A negative public reaction is expected to oppose plans of the British energy industry to take valuable sites for industrial and commercial projects on the ground that government demand forecasts are too high and that they downplay conservation. The United Kingdom (UK) Department of Energy points out that public inquiry always accompanies energy installations and defends the projections made by producers even though each study emphasizes the demand for its own form of energy. At issue are plans to open 150 opencast coal mines a year to compensate for diminishing oil and gas supplies, onshore drilling by oil and gas exploration teams on nearly 50,000 km/sup 2/, and sites required for onshore pipelines, synthetic natural gas facilities, pumped storage plants, and nuclear power stations and waste management. The sites under discussion raise aesthetic and ecological concerns. (DCK)

1982-03-11

496

Combining innovative technology demonstrations with dense nonaqueous phase liquids cleanup

The evolution of these new ion-exchange resins hinges upon an understanding of the chemistry of actinide metal complexes as they exist in solution and when interacting with the cationic sites. Molecular modeling of electrostatic interactions between the metal complex and the cationic substrate is used to help visualize uptake mechanisms and, eventually, to predict optimized coordination sites. Thus, these new materials integrate the fields of ion-specific chelation and ion-exchange technology. Synthesized resins contain cationic sites designed to facilitate the uptake of plutonium nitrato complexes from high-nitrate solutions, and they exhibit distribution coefficients that are up to 10 times higher than those observed for commercial resins. Additionally, some new resins show surprisingly large Kd`s for anionic complexes of Am(III) and U(VI).

1997-12-31

497

Characterization of acid sites in pentasil zeolites by x-ray photolectron spectroscopy

Radioactively contaminated acidic aqueous wastes and organic liquids were discharged to the soil column at three disposal sites within the 200 West Area of the Hanford Site, Washington. As a result, a portion of the underlying groundwater is contaminated with carbon tetrachloride several orders of magnitude above the maximum contaminant level accepted for a drinking water supply. Treatability testing and cleanup actions have been initiated to remove the contamination from both the unsaturated soils to minimize further groundwater contamination and the groundwater itself. To expedite cleanup, innovative technologies for (1) drilling, (2) site characterization, (3) monitoring, (4) well field development, and (5) contaminant treatment are being demonstrated and subsequently used where possible to improve the rates and cost savings associated with the removal of carbon tetrachloride from the soils and groundwater.

1993-05-01

498