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2008-01-01
In this article we present a practical focus for the differential diagnosis of hypophosphataemic disorders inherited join to the osteomalacia induced by tumor (an acquired form). Deepening about the x linked hypophosphataemic rickets and we present description of family with the diagnosis
1992-05-15
Inside this work a general balance is made on the Leak before break approach (LBB), like they are the history and licensing, those limitations, the acceptance criteria of the LBB, the current state of the regulatory development and the current state of it application in countries like E.U.A, Germany, Canada, Italy and France. Everything it with the purpose of evaluating the opportunities that offers the LBB criteria for it possible application in Mexico. (Author)
2007-07-01
It is shown in alive, the capacity of the methyl gallate to reduce the induced ruptures in the DNA for {gamma} radiation. As well as to capture free radicals in a system in vitro. This suggests that the methyl gallate can be a radioprotector that acts capturing free radicals. (Author)
2001-06-01
Closed loop obstruction of the bowel is an extremely grave condition that may lead to serious consequences and even be fatal. The authors describe a case of a patient with intestinal obstruction secondary to cancer of the sigmoid and highlight the importance of computed tomography in the analysis of the pathological changes in routine management of patients with acute abdomen. (author)
2001-01-01
Closed loop obstruction of the bowel is an extremely grave condition that may lead to serious consequences and even be fatal. The authors describe a case of a patient with intestinal obstruction secondary to cancer of the sigmoid and highlight the importance of computed tomography in the analysis of the pathological changes in routine management of patients with acute abdomen. (author)
2008-01-01
Knee injuries, both acute and chronic are a frequent cause of consultation in the general population and athletes. In addition to ligamentary and meniscus injuries, there is a large number of diseases that produce knee pain or functional limitation, which are treated differently or may modify the initial treatment. MRI is a very effective method for the study and diagnosis of these pathologies. We describe in this article some of these pathologies found on studies performed at Instituto de Alta Tecnologia Medica de Antioquia (IATM )
2003-07-01
TGN had a rupture in service on Gasoducto Troncal Norte. After initial evaluation of the causes of the rupture it was concluded that it had been caused by Stress Corrosion Cracking (SCC). Subsequent investigation in the area of the rupture revealed that colonies of cracks, typical of SCC were found in pipes located near the rupture. In order to put back in service the pipeline in a safety condition, SCC mitigation activities were performed. A decision was made to conduct a hydro test along approximately 30 kilometers of pipe. The stages of the works, the problems faced and the solutions found are dealt with, as well as the conclusions reached upon completion of the works which allowed a better understanding of SCC phenomenon. The methodology for the identification of those areas susceptible to SCC is also described. (author)
1992-01-01
The corrosion in a general way can be defined like a chemical or electrochemical reaction, which is carried out in the surface of the metallic materials exposed to a specific medium. Due to the operation conditions in the nuclear power plants are practically fixed and its modification in most of the cases is difficult or expensive, the natural tendency to prevent the corrosion has been generally directed toward the selection of materials. Numerous materials have been employees as substitute of the traditional steels, among other the stabilized stainless steels, those of extra low carbon and numerous nickel base alloys. The basic evaluation that establishes the approach Leak before break (LBB) it involves the analysis by means of 'fracture mechanics' of a postulated crevice that it crosses the thickness of the material, which causes a same flight, with a margin of safety, to the ...
1992-05-15
The corrosion in a general way can be defined like a chemical or electrochemical reaction, which is carried out in the surface of the metallic materials exposed to a specific medium. Due to the operation conditions in the nuclear power plants are practically fixed and its modification in most of the cases is difficult or expensive, the natural tendency to prevent the corrosion has been generally directed toward the selection of materials. Numerous materials have been employees as substitute of the traditional steels, among other the stabilized stainless steels, those of extra low carbon and numerous nickel base alloys. The basic evaluation that establishes the approach Leak before break (LBB) it involves the analysis by means of 'fracture mechanics' of a postulated crevice that it crosses the thickness of the material, which causes a same flight, with a margin of safety, to the detection limit that it has for a shutdown of the reactor. Due to the crevice size postulated, it cannot be established highly starting from the mechanical properties of the material since these its will be affected by the corrosion mechanisms that can settle down, it was determined that the implementation of the LBB criteria, it cannot be established for components or systems that its are susceptible of suffering corrosion. (Author)
ANOMALOUS DOPAMINE RELEASE ASSOCIATED WITH A HUMAN DOPAMINE TRANSPORTER CODING VARIANT
2008-07-09
Dopamine (DA) signaling at synapses is tightly coordinated through opposing mechanisms of vesicular fusion-mediated DA release and transporter-mediated DA clearance. Altered brain DA signaling...Full Text Available
2010-01-01
Central dopaminergic (DA) systems are affected during human immunodeficiency virus (HIV) infection. So far, it is believed that they degenerate with progression of HIV disease because deterioration of DA systems is evident in advanced stages of infection. In this manuscript we found that (a) DA levels are increased and DA turnover is decreased in CSF of therapy-nave HIV patients in asymptomatic infection, (b) DA increase does not modulate the availability of DA transporters and D2-receptors, (c) DA correlates inversely with CD4+ numbers in blood. These findings show activation of central DA systems without development of adaptive responses at DA synapses in asymptomatic HIV infection. It is probable that DA deterioration in advanced stages of HIV infection may derive from increased DA av...
32 CFR 635.23 - DA Form 4833 (Commander's Report of Disciplinary or Administrative Action).
...2009-07-01 2009-07-01 false DA Form 4833 (Commander's Report of Disciplinary...REPORTING Offense Reporting 635.23 DA Form 4833 (Commander's Report of Disciplinary...Administrative Action). (a) Use. DA Form 4833 is used with DA Form 3975...
2010-01-01
Micro-organisms that degrade plant biomass require a range of enzymes to effectively degrade such substrates into sugar monomers. Some organisms secrete a host of free, extracellular enzymes while others produce a multi-enzyme complex (MEC) consisting of a variety of these enzymes. This study examined the cellulolytic and hemi-cellulolytic system of Bacillus licheniformis SVD1 with respect to the presence of key enzymes within the MEC found in this organism. When cultured on birchwood xylan, the MEC in B. licheniformis SVD1 was found to contain two endoglucanases (at 25-27kDa, 30-32kDa), seven xylanases (at 20kDa, 38kDa, 42kDa, 45kDa, 48kDa, 54kDa and 70kDa), two mannanases (25kDa, 40-42kDa) and one pectinase (a pectate lyase at 70-72kDa). Identification of enzymes was based on zymogram an...
...implemented by the permittee as a requirement of a DA permit (i.e. , permittee-responsible...established, enhanced, or preserved. DA means Department of the Army. Days...compensatory mitigation requirements for DA permits. Similar to a mitigation...
33 CFR 332.1 - Purpose and general considerations.
...the issuance of Department of the Army (DA) permits pursuant to section 404...the general mitigation requirements for DA permits. In particular, it does not alter...section affects the requirement that all DA permits subject to section 404 of the...
33 CFR 325.1 - Applications for permits.
...part apply to any Department of the Army (DA) permit. Special procedures and additional...Engineers in processing applications for DA permits. (b) Pre-application consultation...of planning for work which may require a DA permit and which may involve...
...applications for Department of the Army (DA) permits to authorize certain structures...regulated under other authorities of the DA. These include discharges of dredged...S.C. 1413; see 33 CFR part 324). A DA permit will also be required under...
33 CFR 320.1 - Purpose and scope.
...applications for Department of the Army (DA) permits for controlling certain activities...various federal statutes which require that DA permits be issued before these activities... (c) Forms of authorization. DA permits for the above described...
14 CFR 91.175 - Takeoff and landing under IFR.
...United States military aircraft. (b) Authorized DA/DH or MDA. For the purpose of this section...procedure being used provides for and requires the use of a DA/DH or MDA, the authorized DA/DH or MDA is the highest of the following:...
alpha2-mediated effect of dopamine on the motility of the chicken esophagus
1990-01-01
Dopamine (DA), apomorphine and B-HT 933 produced dose related contractions on isolated longitudinal strips of chicken esophagus, whereas phenylephrine elicited no effect. DA induced contractions of myogenic origin, these contractions were insensitive to DA antagonists and were partially suppressed by yohimbine, which suggested an alpha2-adrenergic implication in this DA effect. This hypothesis was further investigated by performing binding experiments, in which B-HT 933 displaced the binding of [3H] DA to esophageal homogenates. The results suggest the participation of an alpha2 - adrenergic receptor in the contractile response elicited by DA in the isolated chicken esophagus
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
MO and DA on the SWIE Instrument on the Wind Spacecraft
The construction of the Faraday Cup portion of the SWIE instrument on the Wind spacecraft, participation in Mission Operations, and Data Analysis (MO and DA) of observations of the solar wind has been supported ...
2009-01-01
Hypoglycaemic coma and brain injury are potential complications of insulin therapy. Hippocampal neurons are particularly vulnerable to hypoglycaemic stress leading to memory impairment. In the present article, we have investigated the dopamine (DA) content, homovanillic acid (HVA)/DA turnover ratio, DA D1 and DA D2 receptors in the hippocampus of insulin-induced hypoglycaemic (IIH) and streptozotocin induced diabetic rats where brain functions are impaired. The DA content decreased significantly in hippocampus of diabetic, diabetic +IIH and control +IIH rats compared to control. The HVA/DA turnover ratio also increased significantly in diabetic, diabetic +IIH and control +IIH rats compared to control. Scatchard analysis using [3H] DA in the hippocampus showed a significant increase in DA r...
Apomorphine and the dopamine hypothesis of schizophrenia: a dilemma?
2001-05-01
The dopamine (DA) hypothesis of schizophrenia implicates an enhancement of DA function in the pathophysiology of the disorder, at least in the genesis of positive symptoms. Accordingly, apomorphine,...Full Text Available
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
1991 DA: An asteroid in a bizarre orbit
Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that ...
Histamine and histidine effects on the transport of dopamine in the rat brain synaptosomes
1992-12-31
The effects of histamine (HA) on dopamine (DA) transport in rat brain synaptosomes has been studied. The exogenous HA inhibited the veratridine-evoked DA release, but had no effect on the DA uptake. A drop of DA uptake to synaptosomes under the conditions favoring histamine synthesis from histidine was connected with histidine itself, but not with the formed histamine. (author). 20 refs, 5 figs.
2009-01-01
The expression `dental age' (DA) denotes the mean age at which a given stage of dental development is achieved in a population chosen as calibrator. The rhythm at which the maturation occurs is a characteristic of a given population in a given period. When DA is determined on subjects of a population different from the calibrator, the shape of the relationship of DA to chronological age (CA) must be empirically determined to trace reference charts for DA that properly apply to this population. The aim of this study is to show how the DA determined in a calibration set (Haavikkos DA) can be adapted to apply to another population (Italian children), by means of CA-dependent smooth functions of the location, scale and shape of DA distribution. A set of 492 good quality panoramic films taken o...
A PanDA backend for the ganga analysis interface
Ganga provides a uniform interface for running ATLAS user analyses on a number of local, batch, and grid backends. PanDA is a pilot-based production and distributed analysis system developed and used extensively by ATLAS. This work presents the implementation and usage experiences of a PanDA backend for Ganga. Built upon reusable application libraries from GangaAtlas and PanDA, the Ganga PanDA backend allows users to run their analyses on the worldwide PanDA resources, while providing the ability for users to develop simple or complex analysis workflows in Ganga. Further, the backend allows users to submit and manage "personal" PanDA pilots: these pilots run under the user's grid certificate and provide a secure alternative to shared pilot certificates while enabling the usage of local resource allocations
Two cortical rod proteins having molecular weights of 28.6 kDa and 30.5 kDa were isolated from the mature ovary of Marsupenaeus japonicus using gel filtration and reversed-phase HPLC. Analysis of the N-terminal amino acid sequence of the 28.6 kDa molecule revealed that amino acid residues 1-21 corresponded to residues 9-29 of the 30.5 kDa molecule. Examination of homology using BLAST showed that 21 amino acids out of 29 residues of the 28.6 kDa molecule, and 14 out of 29 residues of the 30.5 kDa molecule were identical to that of the ovarian cortical rod proteins of Penaeus semisulcatus. Positive immunohistochemical reaction to antiserum raised against the 28.6 kDa protein was observed on cortical rods forming around the periphery of oocytes at the maturation stages. Western blotting analysis revealed that both the 28.6 kDa and 30.5 kDa molecules stained with the anti-28.6 kDa antiserum. Furthermore, the 28.6 kDa and 30.5 kDa proteins were both glycosylated, as evidenced by positive carbohydrate staining using Concanavalin A and production of positive PAS reaction. These results indicate that the cortical rods are comprised of the 28.6 kDa and 30.5 kDa molecules. We subsequently cloned two full-length cDNAs based on the N-terminal sequences of the 28.6 kDa and 30.5 kDa molecules. The open reading frame of 28.6 kDa and 30.5 kDa encoded 276 amino acid residues. Comparison analysis of the two cDNAs revealed that the location of the processing site and sequence of signal peptides differed, indicating that the two cDNAs are products of two separate genes and encode the 28.6 kDa molecule and 30.5 kDa molecule, respectively. Both proteins possessed one potential N-linked glycosylation site. It is considered that both molecules are components of the cortical rods, forming a jelly layer after fertilization.
Dopamine receptor subtypes in renal brush border and basolateral membranes.
Dopamine (DA) modulates renal tubular sodium transport by actions at both brush border (BBM) and basolateral membranes (BLM). DA receptors have been demonstrated in proximal tubule but the subtype of DA receptor in either BBM or BLM has not been determined. DA-1 receptors were quantitated by the specific binding of 125I-SCH 23982, a DA-1 antagonist (defined by 20 microM SCH 23390, a DA-1 antagonist) and DA-2 receptors by the specific binding of 3H-methyl-spiroperidol or 3H-spiroperidol (defined by 30 microM trifluperidol, a predominantly DA-2 antagonist). The specific binding of 125I-SCH 23982 and 3H-methyl-spiroperidol or 3H-spiroperidol were saturable with time and ligand concentration and reversible. Analysis of Rosenthal plots by non-linear regression revealed a high affinity site and a very low affinity site for both BLM and BBM. Maximum receptor density was similar in BBM and BLM. Competition experiments with 125I-SCH 23982 revealed high and low affinity binding sites in both BBM and BLM. The high affinity site was characteristic of a DA-1 receptor. Competition experiments with 3H-spiroperidol were also suggestive of DA-2 receptors. DA-1 but not DA-2 drugs increased adenylate cyclase and phospholipase-C activities in both BBM and BLM. However, their effects were greater in BLM than BBM. We conclude that DA-1 and DA-2 receptors are present in both BBM and BLM in canine kidney. Renal DA-1 receptors are linked to stimulation of both adenylate cyclase and phospholipase-C activity.
Dopamine receptor subtypes in renal brush border and basolateral membranes
Dopamine (DA) modulates renal tubular sodium transport by actions at both brush border (BBM) and basolateral membranes (BLM). DA receptors have been demonstrated in proximal tubule but the subtype of DA receptor in either BBM or BLM has not been determined. DA-1 receptors were quantitated by the specific binding of 125I-SCH 23982, a DA-1 antagonist (defined by 20 microM SCH 23390, a DA-1 antagonist) and DA-2 receptors by the specific binding of 3H-methyl-spiroperidol or 3H-spiroperidol (defined by 30 microM trifluperidol, a predominantly DA-2 antagonist). The specific binding of 125I-SCH 23982 and 3H-methyl-spiroperidol or 3H-spiroperidol were saturable with time and ligand concentration and reversible. Analysis of Rosenthal plots by non-linear regression revealed a high affinity site and a very low affinity site for both BLM and BBM. Maximum receptor density was similar in BBM and BLM. Competition experiments with 125I-SCH 23982 revealed high and low affinity binding sites in both BBM and BLM. The high affinity site was characteristic of a DA-1 receptor. Competition experiments with 3H-spiroperidol were also suggestive of DA-2 receptors. DA-1 but not DA-2 drugs increased adenylate cyclase and phospholipase-C activities in both BBM and BLM. However, their effects were greater in BLM than BBM. We conclude that DA-1 and DA-2 receptors are present in both BBM and BLM in canine kidney. Renal DA-1 receptors are linked to stimulation of both adenylate cyclase and phospholipase-C activity.
Dopamine receptor subtypes in renal brush border and basolateral membranes
1989-08-01
Dopamine (DA) modulates renal tubular sodium transport by actions at both brush border (BBM) and basolateral membranes (BLM). DA receptors have been demonstrated in proximal tubule but the subtype of DA receptor in either BBM or BLM has not been determined. DA-1 receptors were quantitated by the specific binding of 125I-SCH 23982, a DA-1 antagonist (defined by 20 microM SCH 23390, a DA-1 antagonist) and DA-2 receptors by the specific binding of 3H-methyl-spiroperidol or 3H-spiroperidol (defined by 30 microM trifluperidol, a predominantly DA-2 antagonist). The specific binding of 125I-SCH 23982 and 3H-methyl-spiroperidol or 3H-spiroperidol were saturable with time and ligand concentration and reversible. Analysis of Rosenthal plots by non-linear regression revealed a high affinity site and a very low affinity site for both BLM and BBM. Maximum receptor density was similar in BBM and BLM. Competition experiments with 125I-SCH 23982 revealed high and low affinity binding sites in both BBM and BLM. The high affinity site was characteristic of a DA-1 receptor. Competition experiments with 3H-spiroperidol were also suggestive of DA-2 receptors. DA-1 but not DA-2 drugs increased adenylate cyclase and phospholipase-C activities in both BBM and BLM. However, their effects were greater in BLM than BBM. We conclude that DA-1 and DA-2 receptors are present in both BBM and BLM in canine kidney. Renal DA-1 receptors are linked to stimulation of both adenylate cyclase and phospholipase-C activity.
PI3K Signaling Supports Amphetamine-induced Dopamine Efflux
2008-08-08
Full Text Available.The dopamine (DA) transporter (DAT) is a major molecular target of the psychostimulant amphetamine (AMPH). AMPH, as a result of its ability to reverse DAT-mediated inward transport of DA, induces DA efflux thereby increasing extracellular DA levels. This increase is thought to underlie the behavioral effects of AMPH. We have demonstrated previously that insulin, through phosphatidylinositol 3-kinase (PI3K) signaling, regulates DA clearance by fine-tuning DAT plasma membrane expression. PI3K signaling may represent a novel mechanism for regulating DA efflux evoked by AMPH, since only active DAT at the plasma membrane can efflux DA. Here, we show in both a heterologous expression system and DA neurons that inhibition of PI3K decreases DAT cell surface expression and, as a consequence, AMPH-induced DA efflux.
alpha. sub 2 -mediated effect of dopamine on the motility of the chicken esophagus
Dopamine (DA), apomorphine and B-HT 933 produced dose related contractions on isolated longitudinal strips of chicken esophagus, whereas phenylephrine elicited no effect. DA induced contractions of myogenic origin, these contractions were insensitive to DA antagonists and were partially suppressed by yohimbine, which suggested an {alpha}{sub 2}-adrenergic implication in this DA effect. This hypothesis was further investigated by performing binding experiments, in which B-HT 933 displaced the binding of ({sup 3}H) DA to esophageal homogenates. The results suggest the participation of an {alpha}{sub 2} - adrenergic receptor in the contractile response elicited by DA in the isolated chicken esophagus.
alpha. sub 2 -mediated effect of dopamine on the motility of the chicken esophagus
1990-01-01
Dopamine (DA), apomorphine and B-HT 933 produced dose related contractions on isolated longitudinal strips of chicken esophagus, whereas phenylephrine elicited no effect. DA induced contractions of myogenic origin, these contractions were insensitive to DA antagonists and were partially suppressed by yohimbine, which suggested an {alpha}{sub 2}-adrenergic implication in this DA effect. This hypothesis was further investigated by performing binding experiments, in which B-HT 933 displaced the binding of ({sup 3}H) DA to esophageal homogenates. The results suggest the participation of an {alpha}{sub 2} - adrenergic receptor in the contractile response elicited by DA in the isolated chicken esophagus.
2010-01-01
This study describes the synthesis, the spectroscopic, and the thermal characterization of linear and crosslinked polymers as well as a number of corresponding model compounds, containing Diels-Alder adducts derived from furan and maleimide groups. The thermal reversibility (rDA, DA) of structurally varied model compounds, polymeric and network structures were studied by differential scanning calorimetry, where possible in combination with 1H NMR spectroscopy. It was established that the endo and exo DA stereoisomers show significantly different thermal responses: the rDA of the endo DA-adducts typically takes place at 20-40 K lower temperatures than that of the corresponding exo DA-adducts in all cases, with the exception of some aromatic maleimides. Although in situ isomerization was obs...
Spectroscopic analysis of DA white dwarfs from the McCook and Sion catalog
2009-01-01
For some years now, we have been gathering optical spectra of DA white dwarfs in an effort to study and define the empirical ZZ Ceti instability strip. However, we have recently expanded this survey to include all the DA white dwarfs in the McCook and Sion catalog down to a limiting visual magnitude of V = 17.5. We present here a spectroscopic analysis of over 1000 DA white dwarfs from this ongoing survey. We have several specific areas of interest most notably the hot DAO white dwarfs, the ZZ Ceti instability strip, and the DA+dM binary systems. Furthermore, we present a comparison of the ensemble properties of our sample with those of other large surveys of DA white dwarfs, paying particular attention to the distribution of mass as a function of effective temperature
2010-01-01
A11 diencephalospinal dopamine (DA) neurons provide the major source of DA innervation to the spinal cord. DA in the dorsal and ventral horns modulates sensory, motor, nociceptive, and sexual functions. Previous studies from our laboratory revealed a sex difference in the density of DA innervation in the lumbar spinal cord. The purpose of this study was to determine whether sex differences in spinal cord DA are androgen dependent, influenced by adult or perinatal androgens, and whether a sex difference in the number of lumbar-projecting A11 neurons exists. Adult male mice have significantly higher DA concentrations in the lumbar spinal cord than either females or males carrying the testicular feminization mutation (tfm) in the androgen receptor (AR) gene, suggesting an AR-dependent origin....
2010-01-01
Dopamine (DA) neurons in the ventral tegmental area (VTA) have been implicated in brain mechanisms related to motivation, reward, and drug addiction. Successful identification of these neurons in vitro has historically depended upon the expression of a hyperpolarization-activated current (Ih) and immunohistochemical demonstration of the presence of tyrosine hydroxylase (TH), the rate-limiting enzyme for DA synthesis. Recent findings suggest that electrophysiological criteria may be insufficient for distinguishing DA neurons from non-DA neurons in the VTA. In this study, we sought to determine factors that could potentially account for the apparent discrepancies in the literature regarding DA neuron identification in the rodent brain slice preparation. We found that confirmed DA neurons fro...
Imaging dopamine transmission in schizophrenia
1998-01-01
Over the last ten years, several positron emission tomography (PET) and single photon computerized tomography (SPECT) studies of the dopamine (DA) system in patients with schizophrenia were performed to test the hypothesis that DA hyperactivity is associated with this illness. In this paper are reviewed the results of fifteen brain imaging studies comparing indices of DA function in drug naive or drug free patients with schizophrenia and healthy controls: thirteen studies included measurements of Da D2 receptor density, two studies compared amphetamine-induced DA release, and two studies measured DOPA decarboxylase activity, an enzyme involved in DA synthesis. It was conducted a meta-analysis of the studies measuring D2 receptor density parameters, under the assumption that all tracers labeled the same population of D2 ...
Final report: Efficient and user friendly C++ library for differential algebra
1998-09-29
In Phase I we proposed the following tasks: Task 1: Identify the units of the Differential Algebra (DA) library, i.e. determine the abstract data types of the DA and the relations between them. Develop the interfaces (.h files) for the DA vectors. Task 2: Implement the DA vector class with garbage collection and expression templates for optimizing all overloaded operators by minimizing creation of temporaries and fusing loops. Task 3: Implement the prototype GUI for instantiating systems from files and invoking the DA methods. Task 4: Develop a suite of tests for the DA vector class and the needed utilities classes. Task 5: Write the final report on this work. This will include documentation on the use of the code. We have completed these tasks. In this section we discuss the results of our work.
2002-10-01
We investigated the effect of ginseng total saponin (GTS) on nicotine-induced dopamine (DA) release in the striatum and nucleus accumbens of freely moving rats using in vivo microdialysis technique. Systemic pretreatment with GTS decreased striatal DA release induced by local infusion of nicotine into the striatum. However, GTS had no effect on the resting levels of extracellular DA in the striatum. GTS also blocked nicotine-induced DA release in the nucleus accumbens. The results of the present study suggest that GTS acts on the DA terminals to prevent DA release induced by nicotine. This may reflect the blocking effect of GTS on behavioral hyperactivity induced by psychostimulants.
2002-01-01
We investigated the effect of ginseng total saponin (GTS) on nicotine-induced dopamine (DA) release in the striatum and nucleus accumbens of freely moving rats using in vivo microdialysis technique. Systemic pretreatment with GTS decreased striatal DA release induced by local infusion of nicotine into the striatum. However, GTS had no effect on the resting levels of extracellular DA in the striatum. GTS also blocked nicotine-induced DA release in the nucleus accumbens. The results of the present study suggest that GTS acts on the DA terminals to prevent DA release induced by nicotine. This may reflect the blocking effect of GTS on behavioral hyperactivity induced by psychostimulants
Crustacean dopamine receptors: localization and G protein coupling in the stomatogastric ganglion
2008-01-01
Abstract Neuromodulators, such as dopamine (DA), control motor activity in many systems. To begin to understand how DA modulates motor behaviors, we study a well-defined model: the crustacean stomatogastric nervous system (STNS). The spiny lobster STNS receives both neuromodulatory and neurohormonal dopaminergic input, and extensive background information exists on the cellular and network effects of DA. However, there is a void of information concerning the mechanisms of DA signal transduction in this system. In this study, we show that Gs, Gi, and Gq are activated in response to DA in STNS membrane preparations from five crustacean species representing distant clades in the order Decapoda. Three evolutionarily conserved DA receptors mediate this response in spiny lobsters: D1aPan, D1bPan...
Characterization of a sex pheromone in a simultaneous hermaphroditic shrimp, Lysmata wurdemanni
2010-01-01
Olfactory chemical cues have been described to play important roles in the control of mate recognition in many decapod crustaceans. However, we still know very little about the chemical characteristics of the cues that coordinate pre-copulative behaviour. In this study, we partially characterized a waterborne sex pheromone of a marine shrimp, Lysmata wurdemanni. Female moulting water was collected and ultrafiltered using 1,000 and 500-Da membranes, respectively, and analysed using HPLC with a Lichrosphere RP18 (C18) column. The sex pheromone is likely to be a molecule between 500 and 1,000Da in size because behavioural bioassays showed that males responded to the supernatant of 500-Da and to the 1,000-Da filtrate, but did not respond to the supernatant of 1,000-Da or to the 500-Da fil...
2010-01-01
This study investigated the changes in pathogenicity of relapsed diminazene aceturate (DA)-resistant Trypanosoma brucei brucei (TBB) as the trypanosomes are transmitted from DA-treated hosts to another set of animals. The Federe strain of T. brucei brucei which was known to be DA resistant was used to infect two groups of rats, one of which was treated at the peak of infection. There was temporary clearance of the trypanosomes from blood of the treated group and subsequent relapse of the infection. The relapsed trypanosomes were used to infect a group of rats (group A1), and the pathogenicity of the relapsed T. brucei brucei in this rat group was compared to that of a group infected with primary T. brucei brucei stock that was not treated with DA (group B1). Results showed that when compar...
2008-10-15
The rapid and reliable estimation of moment magnitude M{sub w}, location, and size of rupture area, and radiated energy E{sub s} of great Mexican subduction zone earthquakes is critical for a quick assessment of tsunami and/or damage potential of the event and for issuing an early tsunami alert. To accomplish this goal, the Mexican broadband seismic network needs to be supplemented by permanent GPS stations along the Pacific coast, spaced about 65 km apart or less. The data from the GPS network must be transmitted to a central location and processed in near-real time to track the position of the stations. Assuming that this can be implemented, we develop a procedure for near-real time estimation of the critical source parameters. We demonstrate the viability of the procedure by processing near-source GPS data and regional seismograms for the earthquakes of Colima-Jalisco in 1995 (M{sub w}=8.0) and Sumatra-Andaman in 2004 (M{sub w}=9.0-9.3). The procedure yields estimates of M{sub w} and E{sub s} in excellent agreement with those reported from earlier solutions. In the case of the Colima-Jalisco earthquake, the estimated location and size of rupture area agree with that mapped from aftershock locations. Presently, there are 13 permanent GPS stations along the Pacific coast of Mexico with an average spacing of {approx}200 km which operate in an autonomous mode. It is urgent to increase the number of stations to {>=}28 thus decreasing the spacing of stations to {<=}65 km. Data must be transmitted in near-real time to a central station to track the position of the stations, preferably every second. [Spanish] Para una estimacion oportuna del potencial de dano y tsunami asociado a los grandes temblores de subduccion en Mexico, resulta critica la determinacion rapida y confiable de parametros sismologicos como lo son la magnitud de momento (M{sub w}), la energia sismica radiada (E{sub s}) y la localizacion y el tamano de la ruptura. Para alcanzar este objetivo, la red sismologica de banda ancha mexicana necesita ser complementada con estaciones GPS permanentes localizadas a lo largo de la costa del Pacifico mexicano espaciadas, por lo menos, cada 65 km. Los datos de esta red GPS requieren ser transmitidos a una estacion central y procesados en tiempo real para monitorear la posicion de las estaciones. Asumiendo que lo anterior estuviera implementado, en este trabajo desarrollamos una metodologia para la estimacion rapida de parametros criticos de la fuente sismica y demostramos su viabilidad aplicandola al analisis del sismo de Colima-Jalisco de 1995 (M{sub w}=8.0) y de Sumatra-Andaman de 2004 (M{sub w}=9.0-9.3). Para este par de temblores, la metodologia propuesta arrojo valores de M{sub w} y E{sub s} que son muy similares a los reportados anteriormente, obtenidos a partir de analisis detallados. En el caso del sismo de Colima-Jalisco la localizacion estimada y el tamano del area de ruptura estan de acuerdo con los valores que se pueden inferir a partir de las localizaciones de las replicas. Actualmente existen 13 estaciones GPS permanentes a lo largo de la costa del Pacifico mexicano con un espaciamiento promedio de {approx}200 km, las cuales operan de manera autonoma. Es urgente incrementar este numero de estaciones a {>=}28 (logrando un espaciamiento de {<=}65 km), asi como transmitir los datos y determinar la posicion de las estaciones en tiempo real (preferiblemente cada segundo).
Extracellular single-unit recordings and iontophoresis were used to compare the effect of a single administration of pertussis toxin (PTX, 1 microg), into midbrain dopamine (DA) nuclei (A9 and A10 regions), on the muscarinic, NMDA and DA receptor responses of midbrain DA cells in the anesthetized rat. Iontophoretic applications of DA, or apomorphine (50 microg/kg, i.v.), markedly reduced the firing of DA cells in control rats. In PTX-treated animals, these inhibitory responses were totally abolished, indicating that, in both DA nuclei, the inhibitory DA receptors are coupled to Gi/o proteins. In parallel, there was a significant decrease in the number of active DA cells per track which returned to baseline 5 weeks after the treatment. Applications of the muscarinic agonist oxotremorine M (OXO M) or of NMDA produced a potent increase in the firing of DA cells in control rats. DA neurons treated with PTX were still responsive to OXO M, although their sensitivity to the agonist was significantly reduced by 40%. In contrast, NMDA-induced activation remained unchanged, indicating that PTX did not non-selectively dampen all excitatory responses. Applications of cell-permeable cAMP derivatives did not change the basal firing of DA neurons. On the other hand, the phospholipase C inhibitors neomycin and ET-18-OCH3 (200 microg, i.c.v.), reduced significantly the activation of DA cells induced by OXO M. These data suggest that muscarinic activation of DA cells involves an M1-like receptor, possibly coupled to Gq/11 proteins, but also the participation of a PTX substrate.
Extracellular truncated influenza virus nucleoprotein.
In the culture medium of MDCK cells infected with influenza A/Duck/Ukraine/1/63(H3N8) virus two kinds of virus nucleoprotein (NP) are detected: full-length 56 kDa NP and truncated 53 kDa NP. However, in infected cells 53 kDa NP may be detected only at short pulse and after 10 min chase it becomes nondetectable. The extracellular truncated 53 kDa NP is detected in free RNP, and not in the virions. Both extracellular free 53 and 56 kDa NP in the virions are completely oligomerized. Several data argue against the possibility of extracellular 53 kDa NP formation being a result of extracellular 56 kDa NP proteolytic degradation. Thus, the accumulation of extracellular 53 kDa NP takes place only in the course of infection, and the amount of 53 kDa NP is not increased during prolonged storage of cell-free culture medium at +37 degrees C. Moreover, all extracellular 56 kDa NP of A/Duck/Ukraine/1/63 influenza virus is present in the oligomeric form, and the latter, in contrast to the mononeric form, is highly resistant to proteases. The possibility is discussed that in the course of A/Duck/Ukraine/1/63 (H3N8) influenza virus infection a fraction of the synthesized 56 kDa monomeric NP undergoes the proteolytic cleavage in the infected cells before oligomerization and forms the 53 kDa NP. This 53 kDa NP is then oligomerized, enters the RNP and is quickly secreted from the cells.
RATIONALE: Acute systemic administration of salvinorin A, a naturally occurring kappa-opioid receptor (KOPr) agonist, decreases locomotion and striatal dopamine (DA) overflow. OBJECTIVES: Conventional and quantitative microdialysis techniques were used to determine whether salvinorin A infusion into the dorsal striatum (DSTR) decreases DA overflow by altering DA uptake or release. The influence of repeated salvinorin A administration on basal DA dynamics and cocaine-evoked alterations in DA overflow and locomotion was also assessed. MATERIALS AND METHODS: Salvinorin A was administered via the dialysis probe (0; 20-200 nM) or via intraperitoneal (i.p.) injection (1.0 or 3.2 mg/kg per day x 5 days). The effects of a challenge dose of cocaine were examined 48 h after repeated salvinorin treatment. RESULTS: Retrodialysis of salvinorin A produced a dose-related, KOPr antagonist reversible, decrease in DA levels. Extracellular DA levels were decreased whereas DA extraction fraction, which provides an estimate of DA uptake, was unaltered. In contrast to its acute administration, repeated salvinorin A administration did not modify dialysate DA levels. Similarly, neither basal extracellular DA levels nor DA uptake was altered. Unlike synthetic KOPr agonists, prior repeated administration of salvinorin A did not attenuate the locomotor activating effects of an acute cocaine (20 mg/kg, i.p.) challenge. However, cocaine-evoked DA overflow was enhanced. CONCLUSIONS: These data demonstrate that acute, but not repeated, salvinorin A administration decreases mesostriatal neurotransmission and that activation of DSTR KOPr is sufficient for this effect. Differences in the interaction of salvinorin and synthetic KOPr agonists with cocaine suggest that the pharmacology of these agents may differ.
Darbepoietin alfa (DA) is a long-acting analogue of erythropoietin that has reduced receptor affinity and enhanced biological activity. Experiments were done to test the hypothesis that correction of anemia in tumor-bearing mice by DA would increase tumor oxygenation and potentiate radiation-induced tumor cell killing. A SCC VII tumor model was used to study tumor responses to fractionated radiation therapy in mice with anemia induced by total body irradiation. Administration of DA reduced the extent and duration of anemia and associated tumor hypoxia, protected the bone marrow cells and prevented the body weight loss from the effect of irradiation, and facilitated the recovery in a time-dependent manner, with the administration of DA prior to total body irradiation having the greatest protective effect. When combined with fractionated radiation therapy, DA increased the tumor growth delay time from 2.7 days for irradiation alone to 7.3 to 10.6 days for combination of DA and irradiation. The effect of DA on tumor responses to fractionated radiation therapy was observed when DA was given 18 to 4 days before starting radiation therapy, but DA was also equally effective as a radiosensitizer when given only 2 hours before fractionated irradiation therapy. Weekly dosing of DA was as efficacious for the enhancement of radiation responses of tumors as biweekly dosing. Similar results were obtained in the RIF-1 fibrosarcoma tumor model. These studies show that DA can effectively correct anemia in tumor-bearing mice and sensitize tumor cells to fractionated radiation therapy. Importantly, DA was also able to sensitize tumors to radiation in mice with uncorrected anemia and hypoxia, suggesting that the effect of DA on radiosensitivity was independent of these factors and a different mechanism of action may be responsible for this effect.
The Role of Pseudo-Endoglucanases in the Evolution of Nematode Cell Wall-Modifying Proteins
2010-01-01
In this article, the characterization and evolution of pseudo-endoglucanases and a putative expansin-like gene in the migratory nematode Ditylenchus africanus are described. Four genes were cloned with a very high similarity to the endoglucanase Da-eng1, which, however, lack a part of the catalytic domain most probably due to homologous recombination. Owing to this deletion, at least one of the catalytic residues of the corresponding protein is missing, and hence these genes are possibly pseudogenes. In two of the pseudo-endoglucanase genes, the deletions cause a frameshift (Da-engdel2, Da-engdel4), while two others (Da-engdel1, Da-engdel3) code for protein sequences with an intact carbohydrate-binding module (CBM). Recombinant proteins for Da-ENG1, Da-ENGDEL1, and Da-ENGDEL3 were demonstr...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Temperature determinations of hot DA white dwarfs using IUE continuum fluxes
Effective temperatures of 15 DA white dwarfs hotter than 20,000 K were derived from low-dispersion far ultraviolet spectra obtained with IUE. The analysis was carried out by comparing the observed far ultraviolet ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
Self-consistent recalibration of IUE determination of hot DA white dwarf effective temperatures
Using low dispersion short and long wavelength camera observations, the spectra of over twenty stars identified as DA white dwarfs, with temperatures in the range 20,000 to 70,000 K were analyzed. In addition to the ...
2010-01-01
Full Text Available.Mesolimbic dopamine (DA) systems play a critical role in tobacco addiction driven by nicotine. Nicotine activates midbrain DA neurons and, consequently, elevates DA concentrations in targets, especially in the nucleus accumbens (NAc) of the ventral striatum. The route of drug administration influences the impact of addictive drugs. Here, we examine whether the nature of the administration alters DA neuron activity and DA concentrations in the NAc. Using unhabituated naïve freely moving rats, microdialysis measurements showed that nicotine administered via needle injection caused greater DA release in the NAc than the same dose administered via an implanted chronic cannula. After habituation to the needle injections, however, there was no significant difference in DA signaling between the needle and cannula routes of administration. Consistent with these microdialysis results after habituation, our in vivo tetrode unit recordings showed no significant difference in midbrain DA neuron activity in response to nicotine delivered by needle or cannula as long as predictive cues were avoided.
1991-10-01
USF is a cellular factor involved in the transcriptional regulation of several cellular and viral promoters. Purified USF HeLa cells (HeLA USF) consists of 43- and 44-kDa polypeptides which show independent binding to a specific DNA element. A cDNA encoding the 43-kDa species has been previously cloned. The authors here that the purified form of bacterially expressed 43-kDa USF (1) exists in solution as a dimer whose formation is greatly favored under reducing conditions, (2) binds to its cognate DNA sequence in a manner indistinguishable from that of HeLa USF, and (3) is as efficient as HeLa USF in stimulating transcription from target is immunologically unrelated to the 43-kDa polypeptide but is associated with it in HeLa cell extracts. These results suggests that the 43-kDa component possesses an intrinsic DNA binding the transcriptional activation potential and that the 44-kDa USF component of the natural USF complex may have some regulatory role.
Quantification of Translocator Protein (18 kDa) in the Human Brain with PET and a Novel Radioligand,
2009-07-01
Full Text Available.Translocator protein (TSPO) (18 kDa), formerly called the peripheral benzodiazepine receptor, is upregulated on activated microglia and macrophages and is, thus, a biomarker of inflammation. We previously reported that an
1999-10-01
Full Text Available.Cleavage of the Cry2Aa1 protoxin (molecular mass, 63 kDa) from Bacillus thuringiensis by midgut juice of gypsy moth (Lymantria dispar) larvae resulted in two major protein fragments: a 58-kDa fragment which was highly toxic to the insect and a 49-kDa fragment which was not toxic. In the midgut juice, the protoxin was processed into a 58-kDa toxin within 1 min, but after digestion for 1 h, the 58-kDa fragment was further cleaved within domain I, resulting in the protease-resistant 49-kDa fragment. Both the 58-kDa and nontoxic 49-kDa fragments were also found in vivo when
New light on Leonardo Da Vinci's faces
2010-07-15
How did Leonardo Da Vinci manage to paint such perfect faces? For the first time a quantitative chemical analysis has been done on seven paintings from the Louvre Museum without extracting any samples. This shows the ...
1992-12-15
Import of proteins into mitochondria involves the cooperation of protein translocation systems in the outer and inner membranes. We have identified a 45-kDa protein at the protein import site of the...Full Text Available
Functional enhancement and protection of dopaminergic terminals by RAB3B overexpression
2009-12-29
In Parkinson's disease (PD), dopaminergic (DA) neurons in the substantia nigra (SN, A9) are particularly vulnerable, compared to adjacent DA neurons within the ventral tegmental area (VTA, A10). Here,...Full Text Available
Equality of symmetrized tensors and the coordinate ring of the flag variety
2010-05-21
In this note we give a transparent proof of a result of da Cruz and Dias da Silva on the equality of symmetrized decomposable tensors. This will be done by explaining that their result follows from the fact that the coordinate ring of a flag variety is a unique factorization domain.
Endogenous dopamine (DA) modulates (3H)spiperone binding in vivo in rat brain
1991-01-01
(3H)spiperone (SPI) binding in vivo, biochemical parameters and behavior were measured after modulating DA levels by various drug treatments. DA releasers and uptake inhibitors increased SPI binding in rat striatum. In other brain areas, the effects were variable, but only the pituitary remained unaffected. Surprisingly, nomifensine decreased SPI binding in frontal cortex. The effects of these drugs were monitored by measuring DA, serotonin (5-HT) and their metabolites in the same rats. The increased SPI binding in striatum was parallel to the locomotor stimulation with the following rank order: amfonelic acid greater than nomifensine greater than D-amphetamine greater than or equal to methylphenidate greater than amineptine greater than bupropion. Decreasing DA levels with reserpine or alpha-methyl-para-tyrosine reduced SPI binding by 45% in striatum only when both drugs were combined. In contrast, reserpine enhanced SPI binding in pituitary. Thus, the amount of releasable DA seems to modulate SPI binding characteristics. It is suggested that in vivo, DA receptors are submitted to dynamic regulation in response to changes in intrasynaptic concentrations of DA.
Dopamine receptor subtypes in renal brush border and basolateral membranes
1989-01-01
Dopamine (DA) modulates renal tubular sodium transport by actions at both brush border (BBM) and basolateral membranes (BLM). DA receptors have been demonstrated in proximal tubule but the subtype of DA receptor in either BBM or BLM has not been determined. DA-1 receptors were quantitated by the specific binding of 125I-SCH 23982, a DA-1 antagonist (defined by 20 microM SCH 23390, a DA-1 antagonist) and DA-2 receptors by the specific binding of 3H-methyl-spiroperidol or 3H-spiroperidol (defined by 30 microM trifluperidol, a predominantly DA-2 antagonist). The specific binding of 125I-SCH 23982 and 3H-methyl-spiroperidol or 3H-spiroperidol were saturable with time and ligand concentration and reversible. Analysis of Rosenthal plots by non-linear regression revealed a high affinity site and a very low affinity site for both BLM and BBM. Maximum receptor density was ...
Comment on 'Braneworld remarks in Riemann-Cartan manifolds'
2009-01-01
In a recent paper (J M Hoff da Silva and da Rocha R 2009 Class. Quantum Grav. 26 055007) it is concluded that the Darmois-Israel junction conditions in the presence of torsion are not modified. We point out that this conclusion is invalid. (comments and replies)
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected X-ray ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Chandra Confirmation of a Pulsar Wind Nebula in DA 495
As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected ...
Cellulases of Penicillium verruculosum
2010-01-01
Nine major cellulolytic enzymes were isolated from a culture broth of a mutant strain of the fungus Penicillium verruculosum: five endo-1, 4-b-glucanases (EGs) having molecular masses 25, 33, 39, 52, and 70 kDa, and four cellobiohydrolases (CBHs: 50, 55, 60, and 66 kDa). Based on amino acid similarities of short sequenced fragments and peptide mass fingerprinting, the isolated enzymes were preliminary classified into different families of glycoside hydrolases: Cel5A (EG IIa, 39 kDa), Cel5B (EG IIb, 33 kDa), Cel6A (CBH II, two forms: 50 and 60 kDa), Cel7A (CBH I: 55 and 66 kDa), Cel7B (EG I: 52 and 70 kDa). The 25 kDa enzyme was identical to the previously isolated Cel12A (EG III). The family assignment was further confirmed by the studies of the substrate specificity of the purified enzyme...
2009-08-01
Traumatic brain injury (TBI) features deficits often ameliorated by dopamine (DA) agonists. We have previously shown deficits in striatal DA neurotransmission, using fast scan cyclic voltammetry...Full Text Available
Brain's DNA Repair Response to Neurotoxicants.
Parkinson's Disease (PD) is associated with death of dopaminergic (DA) neurons in the substantia nigra (SN) of the brain. Military personnel abroad are at a greater risk of exposure to pesticides and toxins which may selectively damage DA neurons in the S...
Beam-beam experience at DA{phi}NE
2001-07-01
This paper summarizes the results of experimental observations and measurements of beam-beam interactions in DA{phi}NE, the Frascati Phi-factory. The achieved results are reported with analysis of present limitations in both single and multibunch operation modes and compared with numerical simulations.
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
Asteroid 1986 DA: Radar evidence for a metallic composition
Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece ...
The effects of four major chlorotriazine metabolites on the constitutive synthesis of the catecholamines dopamine (DA) and norepinephrine (NE) were examined using undifferentiated PC12 cells. NE release and intracellular DA and NE concentrations were quantified following treatme...
1996-01-01
Raman spectroscopy was used to study vibrational relaxation of completely symmetrical stretching vibration nu1(CD) 2117 cm-1 of deuterated acetonitrile molecules (DA) in solutions of tetrabutylammonium iodide and bromide, tetraethylammonium chloride, lithium iodide and bromide and sodium iodide. Times and correlation functions of vibrational relaxation of non coordinated DA molecules were of tained, as well as of DA molecules, coordinated with anions, DA molecules, coordinated with anions, entering the composition of contact ion pairs. Influence of anion and cation nature on relaxation parameters was analyzed
Ultrasound detection of foreign body and gas contamination of a penetrating wound
2009-01-01
Sommario Descriviamo un caso di ferita penetrante superficiale contaminata da microbolle d'aria, studiato con esame ecografico in Pronto Soccorso. Le microbolle d'aria sono caratterizzate da piccole dimensioni, distribuzione lungo il tragitto della ferita e da peculiari movimenti "a bollicine di spumante". L'ecografia consente di documentarne la scomparsa nelle ore successive, confermandone la natura traumatica ed escludendo una potenziale complicanza infettiva da germi anaerobi.
1996-07-01
This work presents the authors experience on the viability of FURNAS partnership with the Nacional Energetica for completion of the Serra da Mesa power plant. The partnership refers to the transmission system use for distribution of 51.54 % of the partner power to various consumers in different points of the system. Special emphasis is placed on the evaluation of the transactions with LIGHT-R J and the Brasilia electric company.
In intestinal microvilli, the 110K-calmodulin complex is the major component of the cross-bridges which connect the core bundle of actin filaments to the membrane. The authors previous work showed that the 110-kDa polypeptide can be divided into three functional domains: a 78-dDa fragment that contains the ATPase activity and the ATP-reversible F-actin-binding site, a 12-kDa fragment required for binding calmodulin molecules, and a terminal 20-kDa domain of unkown function. By analysis of limited {alpha}-chymotryptic cleavage products, they now show that the molecular organization is very similar to that described for the S{sub 1} fragment of myosin. The catalytic site was identified by photoaffinity labeling with (5,6-{sup 3}H)UTP, and fragments binding F-actin were identified by cosedimentation assays. Cleavage of the 78-kDa fragment yielded major fragments of 32 and 45 kDa, followed by cleavage of the 45-kDa fragment to a 40-kDa fragment. Of these, only the 32-kDa fragment was labeled by (5,6-{sup 3}H)UTP. They conclude that the catalytic site is located in the 32-kDa fragment and the F-actin-binding site is present in the 45-kDa fragment; the ability to bind actin is lost upon further cleavage of the 45-kDa fragment to 40 kDa. Peptide sequence analysis revealed that the 45-kDa fragment lies within the molecule and suggests that the 32-kDa fragment is the amino terminus. These results suggest that the 12-kDa calmodulin-binding region confers a Ca{sup 2{plus}}-dependent accessibility to the F-actin-protectable cleavage site in the S{sub 1}-like domain. This is the first evidence for a Ca{sup 2{plus}}-regulated structural change in the S{sub 1}-like domain of the 110K-calmodulin complex.
1990-12-01
In intestinal microvilli, the 110K-calmodulin complex is the major component of the cross-bridges which connect the core bundle of actin filaments to the membrane. The authors previous work showed that the 110-kDa polypeptide can be divided into three functional domains: a 78-dDa fragment that contains the ATPase activity and the ATP-reversible F-actin-binding site, a 12-kDa fragment required for binding calmodulin molecules, and a terminal 20-kDa domain of unkown function. By analysis of limited {alpha}-chymotryptic cleavage products, they now show that the molecular organization is very similar to that described for the S{sub 1} fragment of myosin. The catalytic site was identified by photoaffinity labeling with (5,6-{sup 3}H)UTP, and fragments binding F-actin were identified by cosedimentation assays. Cleavage of the 78-kDa fragment yielded major fragments of 32 and 45 kDa, followed by cleavage of the 45-kDa fragment to a 40-kDa fragment. Of these, only the 32-kDa fragment was labeled by (5,6-{sup 3}H)UTP. They conclude that the catalytic site is located in the 32-kDa fragment and the F-actin-binding site is present in the 45-kDa fragment; the ability to bind actin is lost upon further cleavage of the 45-kDa fragment to 40 kDa. Peptide sequence analysis revealed that the 45-kDa fragment lies within the molecule and suggests that the 32-kDa fragment is the amino terminus. These results suggest that the 12-kDa calmodulin-binding region confers a Ca{sup 2{plus}}-dependent accessibility to the F-actin-protectable cleavage site in the S{sub 1}-like domain. This is the first evidence for a Ca{sup 2{plus}}-regulated structural change in the S{sub 1}-like domain of the 110K-calmodulin complex.
Long-term evolution of 1991 DA: A dynamically evolved extinct Halley-type comet
The long-term dynamical evolution of 21 variational orbits for the intermediate-period asteroid 1991 DA was followed for up to /-10(exp 5) years from the present. 1991 DA is close to the 2:7 resonance with ...
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