UK PubMed Central (United Kingdom)

Recent research has implicated the nucleus accumbens (NAc) in consolidating recently acquired goal-directed appetitive memories, including spatial learning and other instrumental processes. However,...Full Text Available

UK PubMed Central (United Kingdom)

Acute inflammation is important for tissue repair; however, chronic inflammation contributes to neurodegeneration in Alzheimer’s disease (AD) and occurs when glial cells undergo prolonged...Full Text Available

UK PubMed Central (United Kingdom)

Antibodies to myelin oligodendrocyte glycoprotein (MOG) have been implicated in Multiple Sclerosis demyelination through activation of complement and/or macrophage-effector processes. We presented...Full Text Available

UK PubMed Central (United Kingdom)

Gastrin-releasing peptide receptor (GRPR), a member of the G protein-coupled receptor superfamily, has been utilized for receptor-mediated targeting of imaging and therapeutic agents; here we extend...Full Text Available

UK PubMed Central (United Kingdom)

BACKGROUND: H2-histamine receptors mediate a wide range of physiological functions extending from stimulation of gastric acid secretion to induction of human promyelocyte differentiation. We have previously...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundCannabinoids represent unique compounds for treating tumors, including astrocytomas. Whether CB₁ and CB₂ receptors mediate this therapeutic effect...Full Text Available

UK PubMed Central (United Kingdom)

Bovine leukemia virus (BLV), a retrovirus related to human T-cell leukemia virus types 1 and 2, can induce persistent nonneoplastic expansion of the CD5⁺ B-cell population, termed...Full Text Available

UK PubMed Central (United Kingdom)

Gonadotropin–releasing hormone (GnRH) neurons are the central regulators of reproduction. GABAergic transmission plays a critical role in pubertal activation of pulsatile GnRH secretion....Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The effects of muscarinic agonists and diisopropylfluorophosphate (DFP) on muscarinic receptor density and muscarinic receptor-mediated responses was assessed in mouse brain and mouse neuroblastoma cells (clone N1E-115). Utilizing the antagonist ({sup 3}H)quinuclidinyl benzilate (({sup 3}H)QNB), there was no difference in the maximal binding capacity (B{sub max}) or equilibrium dissociation constant (K{sub d}) between untreated and 24 hour DFP-treated mice. However, one administration of DFP produced a 24% and 33% decrease in B{sub max} measured by ({sup 3}H)N-methylscopolamine (({sup 3}H)NMS) after 18 and 24 hours which was rapidly reversible within 36 hours after DFP treatment. The loss of ({sup 3}H)NMS binding sites following acute DFP treatment was not accompanied by a change in a particular muscarinic receptor binding conformation. Furthermore, the magnitude of muscarinic receptor-mediated phosphoinositide hydrolysis was unchanged ...

UK PubMed Central (United Kingdom)

Understanding the mechanisms whereby information encoded within patterns of action potentials is deciphered by neurons is central to cognitive psychology. The multiprotein complexes formed by...Full Text Available

UK PubMed Central (United Kingdom)

Hypoxia–ischemia (H/I) in the premature infant leads to white matter injury termed periventricular leukomalacia (PVL), the leading cause of subsequent neurological deficits. Glutamatergic...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe N-methyl-D-aspartate (NMDA)-type glutamate receptor expressed at excitatory glutamatergic synapses is required for learning and memory and is critical for normal brain...Full Text Available

UK PubMed Central (United Kingdom)

Experimental studies have demonstrated that not only dopaminergic signaling but also glutamatergic/NMDA receptor signaling play indispensable roles in the development of methamphetamine psychosis. Our...Full Text Available

International Nuclear Information System (INIS)

Aim: There has been a great demand for developments of the radioligands to visualize the N-methyl-D-aspartate (NMDA) receptors by PET/SPECT. We have recently synthesized two C-11 labeled antagonists for the glycine-binding site on NMDA receptors. The aim of this work is to examine for their in vitro and in vivo binding characteristics, and to evaluate their potentials as PET radioligands for the NMDA receptors. Materials and methods: Two C-11 labeled 4-hydroxy-2-quinolones (1 and 2) were synthesized by conventional methylation of the corresponding phenols with ["1"1C]methyl iodide. In vitro and ex vivo quantitative autoradiographs with imaging plate, as well as animal PET, were employed in order to evaluate their in vitro and in vivo binding to the NMDA receptors. Results: The compound 1 showed the specific binding to rat brain slices with higher localization in the hippocampus and cerebral cortex than ...

Energy Technology Data Exchange (ETDEWEB)

Stargardt`s disease is inherited as an autosomal recessive condition characterized by a juvenile macular dystrophy. Genetic linkage analysis recently assigned the disease locus to chromosome 1p21-p13 with the best estimate for location of the gene near the locus D1S435. We performed linkage analysis in 34 North American families and 2 inbred families from the Kingdom of Saudi Arabia with 12 highly polymorphic markers on chromosome 1p flanking D1S435 between D1S207 and D1S223 and report significant linkage for all 12 markers with no evidence for genetic heterogeneity. Two-point linkage analysis demonstrated the Stargardt`s disease locus and D1S435 are linked with a maximum lod score of 17.17 at a recombination fraction of 1%. The markers UT851, ...

International Nuclear Information System (INIS)

Full text: The maturation of chicken forebrain is protracted and occurs well after synapse formation providing a good model for studying mechanisms of brain maturation. Using microslices from immature (10 day) and adult chicken forebrain prepared after decapitation, we have examined functional properties of NMDA and AMPA receptors by measuring agonist-induced uptake of "4"5Ca"2"+ . The rate and extent of NMDA induced "4"5Ca"2"+ accumulation decreased during maturation with no change in EC_5_0. The rate and extent of the AMPA induced response also decreased with a 60-fold increase in EC_5_0. However, the total NMDA receptor content did not change as indicated by 3 H-MK801 binding and NR1 immunoreactivity in P2 fractions. Similarly, there was no change in the B_m_a_x of "3H-AMPA, though there was a two-fold increase in K_D, and little or no change in the immunoreactivity in GluR1, 2, 2/3 or 4. These results suggest that it is ...

Science.gov (United States)

Elution profiles of total lipoproteins, apolipoprotein B (apoB) concentrations in lipoproteins, and plasma triglyceride (TG) levels were examined in early-, late-, and non-lactating cows. Additionally, arteriovenous (A-V) differences were also measured to elucidate the uptake of TG and apoB-containing lipoproteins in mammary gland. Non-lactating cows showed three major peaks corresponding to triglyceride-rich lipoprotein (TRL), low density lipoprotein (LDL), and high density lipoprotein (HDL) fraction, whereas both early- and late-lactating cows revealed two peaks corresponding to TRL and HDL. The peak area of TRL in early- and late-lactating cows were significantly (p < 0.05) smaller than that in non-lactating cows. The plasma TG levels and apoB-48 concentrations of TRL in early- and late-lactating cows were also significantly (p < 0.01) lower. Furthermore, early lactating cows showed significantly (p < 0.05) larger A-V differences in both plasma TG and apoB-48 concentration ...

Energy Technology Data Exchange (ETDEWEB)

The murine 3T3-L1 fibroblast under appropriate incubation conditions differentiates into an adipocyte phenotype. This 3T3-L1 adipocyte exhibits many of the morphologic, biochemical, and insulin-responsive features of the normal rodent adipocyte. Using quantitative electron microscopic (EM) autoradiography we find that, when /sup 125/I-insulin is incubated with 3T3-L1 adipocytes, the ligand at early times of incubation localizes to the plasma membrane of the cell preferentially to microvilli and coated pits. When the incubation is continued at 37 degrees C, /sup 125/I-insulin is internalized by the cells and preferential binding to the villous surface is lost. With the internalization of the ligand, two intracellular structures become labeled, as determined by the method of hypothetical grain analysis. These include large clear, presumably endocytotic, vesicles and multivesicular bodies. Over the first hour of incubation the labeling of these structures increases in parallel, but in the ...

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During the past year, further studies on mAChR were conducted. These studies included verification of the difference in pituitary distribution based on ligand charge. The pituitary localization of TRB. A neutral mAChR ligand, was verified. The lack of QNB blockade of TRB uptake was tested by blockage with scopolamine, another mAChR antagonist and by testing the effect in a different strain of rat. Neither scopolamine or change of rat strain had any effect. We concluded that TRB uptake in pituitary is not a receptor-mediated process. Further studies were conducted with an additional quaternized mAChR ligand: MQNB. Pituitary localization of MQNB, like MTRB, could be blocked by pretreatment with QNB. We have tentatively concluded that permanent charge on a mAChR antagonist changes the mechanism of uptake in the pituitary. Time course studies and the effects of DES on myocardial uptake are reported. A brief report on preliminary results of evaluation of quaternized ...

International Nuclear Information System (INIS)

Asialoorosomucoid (ASOR) is internalized and degraded by HepG2 cells after binding to the asialoglycoprotein (ASGP) receptor, internalization through the coated pit/coated vesicle pathway, and trafficking to lysosomes. Primaquine, an 8-aminoquinoline antimalarial compound, inhibits ASOR degradation at concentrations greater than 0.2 mM by neutralizing intracellular acid compartments. This leads to alterations in surface receptor number, receptor-ligand dissociation, and receptor recycling. We have investigated the effects of primaquine on 125I-ASOR uptake and degradation as a function of primaquine concentration and duration of exposure. Concentrations below those required for neutralization of acidic compartments block 125I-ASOR degradation in HepG2 cells and lead to intracellular ligand accumulation. This effect is maximal at 80 microM primaquine. The intracellular 125I-ASOR is undegraded, dissociated from the ASGP receptor, and contained within vesicular compartments distinct from ...

British Library Electronic Table of Contents (United Kingdom)

PP2B is a Ca2+/calmodulin-dependent protein phosphatase that is ubiquitously expressed in mammals. Among other actions, it is an effector mechanism in NMDA-mediated glutamate neurotransmission as well as a regulator of GSK3? and MAPK signaling cascades. Because all of these mechanisms have demonstrable roles in the control of circadian rhythyms, we hypothesized that PP2B would be a key regulator of rhythm generation and entrainment, and that through inhibition of its phosphatase activity, the circadian system would be affected by immunosuppressant drug therapy. We report here that immunosuppressant drugs (cyclosporin A, FK506) (1) block the circadian responses to light that underlie photic entrainment; (2) produce circadian phase shifts with a characteristic nonphotic profile; and (3) disr...

CERN Document Server

Tensor network states are used to approximate ground states of local Hamiltonians on a lattice in D spatial dimensions. Different types of tensor network states can be seen to generate different geometries. Matrix product states (MPS) in D=1 dimensions, as well as projected entangled pair states (PEPS) in D>1 dimensions, reproduce the D-dimensional physical geometry of the lattice model; in contrast, the multi-scale entanglement renormalization ansatz (MERA) generates a (D+1)-dimensional holographic geometry. Here we focus on homogeneous tensor networks, where all the tensors in the network are copies of the same tensor, and argue that certain structural properties of the resulting many-body states are preconditioned by the geometry of the tensor network and are therefore largely independent of the choice of variational parameters. Indeed, the asymptotic ...

Energy Technology Data Exchange (ETDEWEB)

Stargardt disease (STGD) and fundus flavimaculatus are infrequent autosomal recessive conditions characterized by a juvenile macular dystrophy and variable degrees of peripheral retinal changes. Linkage analysis performed in 47 STGD/fundus flavimaculatus families demonstrated significant linkage to 13 polymorphic DNA markers on chromosome 1p. The maximum combined two-point lod score was 32.7 (maximum recombination fraction [{theta}{sub max}] = .006) with the polymorphic marker D1S188. Our data demonstrate that STGD and fundus flavimaculatus are the same disorder clinically and genetically and provide further evidence for genetic homogeneity of this phenotype. Analysis of recombination on disease chromosomes placed the STGD gene within a 4-cM interval between markers D1S435 and D1S236. A physical map was constructed of a YAC contig flanking STGD, from markers ...

Science.gov (United States)

Several examples of agonist-directed trafficking of receptor signalling at 5-HT2A and 5-HT2C receptors have been reported that involve independent downstream transduction pathways. We now report the functional selectivity of a series of chemically diverse agonists at human (h)5-HT2A, h5-HT2B and h5-HT2C-VSV by examining two related responses, the upstream activation of Gq/11 proteins in comparison with its associated cascade of calcium mobilisation. At the h5-HT2A receptor, d-lysergic acid diethylamide (LSD) and the antiparkinsonian agents lisuride, bromocriptine and pergolide exhibit a higher potency for Gq/11 activation than calcium release in contrast with all the other tested ligands such as 5-HT, mCPP and BW723C86, that show an opposite preference of signalling pathway. Comparable observations are made at h5-HT2B and h5-HT2C-VSV receptors, suggesting a similar mechanism of functional selectivity for the three serotonin receptors. Interestingly, the non-hallucinogenic compound ...

Science.gov (United States)

The wrms of postfit residuals is 23.003 ps and chi-sq/ndg = 1.089. .... 1555-140 1556-245 1600+43A 1600+43B 1600-489 1606-398 \\ 1608+243 1611-710 1628+216 ...

UK PubMed Central (United Kingdom)

The majority of diseases in the retina are caused by genetic mutations affecting the development and function of photoreceptor cells. The transcriptional networks directing these processes are regulated...Full Text Available

UK PubMed Central (United Kingdom)

Several investigations suggested abnormalities in circadian rhythms are related to the pathophysiology of psychiatric disorders, including drug addiction. Recently, orphan nuclear receptor rev-erb alpha...Full Text Available

International Nuclear Information System (INIS)

The upper range of the requirement for methionine plus cystine in healthy adults was proposed in 1985 by FAO/WHO/UNU to be 13 mg.kg body wt-1.d-1. To explore the validity of this estimate, five healthy, young adult men were given for 7 d a diet based on an L-amino acid mixture supplying 13 mg methionine.kg-1.d-1 (87 mumol.kg-1.d-1) without cystine. Constant intravenous infusions of L-[2H3-methyl-1-13C]methionine were given on days 5 and 7 while subjects were in the fed and postabsorptive states, respectively. Estimates were made of methionine oxidation, and daily methionine balance was derived from the intake-oxidation data. For the five subjects, methionine balances were -0.9, +0.7, +3.5, -3.1, and -3.8 mg kg-1.d-1, or -6, +5, +23, -21, and -26 mumol.kg-1.d-1. These findings ...

Energy Technology Data Exchange (ETDEWEB)

The authors would like to determine |V{sub cb}| from the exclusive semi-leptonic decay B{yields}D*lv. The differential decay rate is d{Lambda}/dw = G{sub F}{sup 2}/4{pi}{sup 3}(w{sup 2}-1){sup 1/2}m{sub D*}{sup 3} (m{sub B}-m{sub D*}){sup 2}G(w)|V{sub cb}|{sup 2}|F{sub B{yields}D*}(w)|{sup 2}, where w = v {center_dot} v{prime} and G(1) = 1. At zero recoil (w = 1) heavy-quark symmetry requires F{sub B{yields}D*}(1) to be close to 1. So, |V{sub cb}| is determined by dividing measurements of d{Lambda}/dw by the phase space and well-known factors, and extrapolating to w {yields} 1. This yields |V{sub cb}|F{sub B{yields}D*}(1), and F{sub B{yields}D*}(1) is taken from ''theory''. To date models [1] or a combination of a rigorous inequality plus judgement [2] have been used to estimate F{sub ...

Energy Technology Data Exchange (ETDEWEB)

Inclusive double spin asymmetries obtained by scattering polarized electrons off polarized protons and deuterons have been analyzed to address the issue of quark-hadron duality in the polarized spin structure functions g^p_1 and g^d_1. A polarized electron beam, solid polarized NH_3 and ND_3 targets and the CEBAF Large Acceptance Spectrometer (CLAS) in Hall B were used to collect the data. The resulting g^p_1 and g^d_1 were averaged over the nucleon resonance energy region (M < W < 2.00 GeV), and three lowest lying resonances individually for tests of global and local duality.

UK PubMed Central (United Kingdom)

The initiation and progression of several forms of retinal degenerations involve excessive, repetitive, and/or sustained oxidative stress that, in turn, mediate photoreceptor cell damage and...Full Text Available

UK PubMed Central (United Kingdom)

Previous independent investigations of the serotyping of Haemophilus parasuis strains have led to the designation of serovars A to D, 1 to 7, Jena 6 to Jena 12, and ND1 to ND5. Heat-stable antigen preparations...Full Text Available

International Nuclear Information System (INIS)

The decay channels of the Ar 2s"-"1 and 2p"-"1 and Kr 3p"-"1 and 3d"-"1 electronic hole states have been investigated by means of photoelectron-photoion coincidence measurements following innershell ionization using synchrotron radiation. With the method of final ion-charge resolving electron spectroscopy it has become possible to disentangle different contributions to the electron spectrum and to determine the decay probabilities P(nl"-"1#->#n+) of the above-mentioned hole states (nl"-"1) to the final ionic charge states n+. A high correlation with threefold or even fourfold charged ions has been found in all cases. Possible decay routes, via cascade or direct double Auger processes, are discussed on the basis of energy-level schemes calculated with the Hartree-Fock method. Special emphasis is laid on the examination of the Kr 3p"-"1 decay process, where the two fine-structure components (j=1/2,3/2) exhibit noticeably different decay ...

Energy Technology Data Exchange (ETDEWEB)

The Chediak-Higashi syndrome (CHS) is a severe autosomal recessive condition, features of which are partial oculocutaneous albinism, increased susceptibility to infections, deficient natural killer cell activity, and the presence of large intracytoplasmic granulations in various cell types. Similar genetic disorders have been described in other species, including the beige mouse. On the basis of the hypothesis that the murine chromosome 13 region containing the beige locus was homologous to human chromosome 1, we have mapped the CHS locus to a 5-cM interval in chromosome segment 1q42.1-q42.2. The highest LOD score was obtained with the marker D1S235 (Z{sub max} = 5.38; {theta} = 0). Haplotype analysis enabled us to establish D1S2680 and D1S163, respectively, as the telomeric and the centromeric flanking markers. Multipoint linkage analysis confirms the ...

Science.gov (United States)

This report updates the status of 32 large projects having EPRI authorizations of $5 million or more. It also describes 86 smaller projects that have EPRI authorizations of less than $5 million and that have a high likelihood of producing a utility-useful new near-term accomplishment within the next two years. Finally, there are 45 examples of analytical, experimental and bench-scale projects that were influential in confirming or redirecting EPRI's research program. These examples cover the entire spectrum of electric R and D: (1) fuel processing, (2) electric power generation, (3) transmission and distribution, (4) energy storage and management, (5) energy analysis, and (6) environmental assessment and control.

International Nuclear Information System (INIS)

This paper describes the heat transfer augmentation and the flow situation around a single spacer (a cylindrical rod) on the heated surface of a parallel plate duct in order to examine basically the effects of the spacer in the fuel elements of a high temperature gas-cooled reactor. The ends of the cylindrical rod contact the upper and lower planes. A thermosensitive liquid crystal film is used to indicate the effective area for the heat transfer. The mean Nusselt number, which is estimated within the optional distance from the spacer to the downstream direction, peaks at a dimensionless distance of X/D = 1-3, and after that decreases gradually with the flow direction. The manner in which heat transfer corresponds to the flow situation is also examined. The horseshoe vortex, produced around the spacer, affects the wake and contributes to the increase of the local heat transfer. (author).

CERN Document Server

Standard micro-economics concentrate on the description of markets but is seldom interested in production. Several economists discussed the concept of a firm, as opposed to an open labour market where entrepreneurs would recrute workers on the occasion of each business opportunity. Coase \\cite{Coase} is one of them, who explains the existence of firms as institution because they reduce the transaction costs with respect to an open labour market. Whatever the rationale proposed by economists to account for the existence of firms, their perspective is based on efficiency and cost analysis. Little attention is paid to the dynamics of emergence and evolution of firms. The aim of the present manuscript is to check the global dynamical properties of a very simple model based on bounded rationality and reinforcement learning. Workers and managers are localised on a lattice and they choose collaborators on the basis of the success of previous work relations. The choice algorithm is largely ...

British Library Electronic Table of Contents (United Kingdom)

Annual wormwood interference on soybean crop growth and yield may result from competition and allelopathy, which are modulated by crop management. Allelochemicals released by annual wormwood (e.g. artemisinin) may affect the crop directly or indirectly through the effect on the nitrogen fixing symbiont, Bradyrhizobium japonicum. The objectives were (i) to quantify the crop response (i.e. biomass production, nodulation and yield) to weed interference and (ii) to determinate the relative change of competition and allelopathy interferences, when a sublethal dose of herbicide is applied. Two split plot field experiments with three replications were used. The experiment involved a factorial combination of five weed-crop density (soybean/annual wormwood, plantsm^-^2) levels: D1, pure soybean, 40...

British Library Electronic Table of Contents (United Kingdom)

In this work, long-term operation of a pilot scale mixed anaerobic reactor processing crude glycerol and rapeseed meal is discussed. These materials are generated as by-products of biodiesel production. Mixed reactor was operated under mesophilic conditions for the period of 654 days. Total cumulative production of biogas reached 379 m3 (at atmospheric pressure and ambient temperature). Maximum volumetric loading achieved during the operation was 2.17 kg m?3 d?1 for the crude glycerol dose of 2 L. When dosing crude glycerol as a single substrate, average specific production of biogas of 0.76 m3 per L of the g-phase was achieved. The lack of nutrients in the g-phase had to be compensated by an addition of ammonium nitrogen in the form of urea into the reactor. Long term processing of crude ...

International Nuclear Information System (INIS)

Environmental factors, phytoplankton biomass (Chl a) and primary production of two water areas in Daya Bay (Dapeng'ao Bay and Aotou Bay) were investigated during the transition period from spring to summer. Chl a ranged from 3.20 to 13.62 and 13.43 to 26.49 mg m"-"3 in Dapeng'ao Bay and Aotou Bay respectively, if data obtained during red tides are excluded. Primary production varied between 239.7 and 1001.4 mgC m"-"2 d"-"1 in Dapeng'ao Bay. The regional distribution of Chl a and primary production were mostly consistent from spring to summer in both bays. Seasonal transition characters have been found in Daya Bay from spring to summer, including high values of DO, nitrate and silicate. Size structures of phytoplankton and its primary production do not change very much from spring to summer, with micro-phytoplankton dominating and contributing about 50% of the whole. In Daya Bay, phytoplankton is limited by nitrogen in spring, and by phosphate ...

International Nuclear Information System (INIS)

The radiation decimal reduction dose (D_1_0) of toxigenic Vibrio cholerae O1 El Tor, Inaba was determined in vitro (0.13 kGy) and in inoculated fresh fillets of saurel (Trachurus picturatus murphyi) (0.12 kGy) and another Pacific fish species known in Peru as ''lisa'', Mugil cephalus (0.13 kGy), both of which are frequently consumed raw in ''ceviche''. The D_1_0 value was similarly determined in tails of the shrimp species Penaeus vannamei (0.13 kGy). In a second phase of the study, radiation doses in the range 1.0-4.0 kGy were evaluated for use in microbiological shelf-life extension of the selected seafood, and for adverse effects on various sensory attributes (appearance, odor, flavor, and texture). A dose of 1.0 kGy doubled the microbiological shelf-life of fish fillets during post-irradiation storage at 0-1 deg. C to approximately 30 days. This dose was deemed optimal also for preserving all ...

International Nuclear Information System (INIS)

The convolution/superposition algorithm for computing dose from photon beams in radiation therapy planning requires knowledge of the energy spectrum. The algorithm can compute the dose for a polyenergetic beam as the weighted sum of the individual dose contributions from monoenergetic beams. In this study we exploit interface effects apparent in the dose distributions to discriminate among spectra of high energy photon beams. We have studied the sensitivity of the depth dose distribution to the energy components using a hypothetical beam for various field sizes and depths in water and water-lung-water media. Six theoretical spectra were simulated. We compared depth dose data from these spectra using three quantitative measures which are inherently free of normalization ambiguities: for homogeneous water, the ratio D_2_0/D_1_0 and a logarithmic derivative in the buildup region LD_b_u_i_l_d_-_u_p and for inhomogeneous lung/water, the lung ...

Energy Technology Data Exchange (ETDEWEB)

Previous karyotypic studies have indicated a possible series of non-random chromosomal events involved in the progression of melanoma. We sought to define a model of melanocyte tumorigenesis by studying allelic deletions of polymorphic simple tandem repeat markers mapping to chromosome 1, 6q, 7, 9p, 10, 11, 17, and 21 in thirty matched pairs of melanoma and constitutional DNAs. The most frequent and earliest deletions were found on 9p (57%) and 10q (32%) and with the exception of one case, no sample has loss of markers on another chromosome without concomitant loss of markers on 9p and/or 10q. Losses on 6q were also a frequent (32%) event that sometimes occurred in primary melanomas, whereas losses of loci on distal 1p (26%) or 11q (26%) occurred only in metastic melanomas. A background rate (0-17%) of allele loss was seen on chromosomes 7, 17, and 21. Homozygous deletions in a panel of 31 melanoma cell lines were only detected for markers on 9p (4 cases). These data strongly support ...

International Nuclear Information System (INIS)

In this work, new LaSi3N5:Ce3+ phosphors have been synthesized by solid-state reaction. Rietveld refinement of the crystal structure of La1-xCexSi3N5 reveals that Ce atoms substituted for La atoms occupy 4a crystallographic positions. Broad emission and excitation bands observed were attributed to the transitions between the doublet ground state of the 4f1 configuration and the crystal field components of the 5d1 excited state. At 77 K, the centroid and crystal field splitting ?cfs of the 5d levels of Ce3+ in LaSi3N5:Ce3+ compounds were valuated at 33.4x103 and 11.3x103 cm-1, respectively. The zero-phonon line and the Stokes shift were measured to be 26.0x103 and 5.0x103 cm-1, respectively.

International Nuclear Information System (INIS)

The energy bands and the nature of the conduction electrons have been studied for two intermetallic compounds of dysprosium, i.e, DyZn and DyRh, following the self-consistent augmented-plane-wave (APW) method. The convergence of energy was found to be rapid for DyZn because the shell of 3d"1"0 electrons for Zn lies below the conduction band. The Fermi energies (E/sub F/) have been calculated to be 0.421 and 0.477 Ry for DyZn and DyRh, respectively. The densities and the numbers of conduction electrons inside and outside the APW spheres in each of these compounds are calculated, and it is shown that the character of conduction electrons inside the APW sphere of Dy is predominantly of d type. In order to see the accuracy of these results, self-consistent calculations were done for DyZn for different choices of the exchange potentials, and the calculated number of conduction electrons did not change significantly. The consequence of the ...

Science.gov (United States)

In a previous study, to identify genes of importance for hepatocellular carcinogenesis, and especially for processes involved in malignant transformation, the authors investigated differences in gene expression between adenomas and carcinomas by DNA microarray. In the present study, the authors investigated AW434047, one of the sequences that was upregulated in carcinomas. The investigation led to the identification of a novel gene, which the authors named hepatocyte malignant transforming factor (HMTF), of unknown function whose expression was increased in hepatocellular carcinomas. Northern blot and in situ hybridization also demonstrated high levels of HMTF in rat hepatocellular carcinoma (HCC) cell lines, lymphocytes in the spleen, colon mucosal epithelia, spermatocytes, and granule cells of the hippocampus. Reduction of HMTF by RNA interference (RNAi) in N1 cells, an HCC cell line, caused suppression of cell proliferation, invasion, and migration. Suppression of proliferation ...

British Library Electronic Table of Contents (United Kingdom)

High surface ozone concentration is increasingly being recognized as a factor that negatively affects crop yields in Asia. However, little progress has been made in developing ozone-tolerant genotypes of rice-Asias major staple crop. This study aimed to identify possible tolerance mechanisms by characterizing two quantitative trait loci (QTLs) that were previously shown to influence visible leaf symptoms under ozone exposure (120 nl l-1, 7 h d-1, 13 d). Two chromosome segment substitution lines (SL15 and SL41) that carried introgressions of the QTLs OzT3 and OzT9, respectively, were exposed to ozone at 120 nl l-1 along with their parent Nipponbare. In accordance with the expected QTL effect, SL15 showed stronger visible symptoms of ozone damage than Nipponbare, whereas SL41 had fewer sympt...

International Nuclear Information System (INIS)

The rates of hydrogen release from simulated high-level liquid waste (SHLLW) during irradiation of "6"0Co #gamma#-rays have been studied using a laboratory-scale apparatus. The SHLLW used was made up of 28 different metal elements, the concentrations of which were determined from a computed data (ORIGEN-2) for a Purex spent fuel (burn-up of 4.5 GWd/t, cooling time of 4 years) except for those of corrosion products (Fe, Cr and Ni) and of chemical process additive (P). The nitrate ion concentration of the SHLLW was 4.97 M. The G-value of hydrogen release was 0.0164 for stirred conditions, but for nonstirred conditions with dose rate : 2.8 kGy/h and with the SHLLW solution height more than 8 cm, the following relation was found between the G-value and the solution height (d cm); G(H_2) = 0.100 d"-"1"."6. The G-values of oxygen and nitrogen releases were also determined. (author).

British Library Electronic Table of Contents (United Kingdom)

Forty synthetic food colors were determined in drinks and candies by reversed-phase high-performance liquid chromatography with photodiode array detection. The following food colors were analyzed within 19min using a short analytical column (50mmx4.6mm i.d., 1.8mm) at 50degreeC with gradient elution: Ponceau 6R, Tartrazine, Fast yellow AB, Amaranth, Indigotine, Naphthol yellow S, Chrysoine, Ponceau 4R, Sunset yellow FCF, Red 10B, Orange G, Acid violet 7, Brilliant black PN, Allura red AC, Yellow 2G, Red 2G, Uranine, Fast red E, Green S, Ponceau 2R, Azorubine, Orange I, Quinoline yellow, Martius yellow, Ponceau SX, Ponceau 3R, Fast green FCF, Eosine, Brilliant blue FCF, Orange II, Orange RN, Acid blue 1, Erythrosine, Amido black 10B, Acid red 52, Patent blue V, Acid green 9, Phloxine B, Ben...

International Nuclear Information System (INIS)

A lot of recent experimental results show that cladding failure limits to the RCCA ejection accident will be changed because of the impact of the high irradiation on the fuel rod behavior in the reactor. The maximal assembly discharge burnup in Daya Bay unit 1 and 2 will reach up to 52 GMd/tU with 18 month fuel cycle. It is necessary to perform the specific RCCA ejection accident analysis for the high burnup fuel assembly in order to evaluate the maximal enthalpy in the fuel rods. There is no definite design limit of maximal enthalpy for high burnup assembly during the RCCA ejection accident. One could perform the rod ejection accident analysis for the high burnup assemblies and compare the analytical results with the specific experimental results. The RCCA ejection accident analysis for the high burnup assemblies for Daya Bay NPS has been performed based on the conventional methodology (2D-1D). For each anticipated cycle in Daya Bay unit 1 and ...

International Nuclear Information System (INIS)

In 620 patients quantitative bone scintigraphy with "9"9"mTc pyrophosphate of the sacroiliac joints and of the spine was performed: 365 patients with confirmed ankylosing spondylitis, 125 patients with clinical radiological suspicion for ankylosing spondylitis (probable Bechterev) and control group of 130 healthy individuals. By comparison of the activity in zones of interest of the sacroiliac joints, the spine and sacrum, the following indices were determined: sacroiliac (separately for the left and right sacroiliac joints), index D_1_0/sacrum, index L_4/sacrum and index C_7/sacrum. The scintigraphic finding was compared to the X-ray one and to the radiological stage of the disease. A long-term follow-up of the patients was carried out during 1-6 years with scintigraphic, X-ray and clinical investigations. Emphasis was laid upon the posibilities for quantitative scintigraphy for early diagnostics of ankylosing spondylitis. The combination of ...

Energy Technology Data Exchange (ETDEWEB)

Two series of diffusion tests were performed to examine the degradation of dichloromethane (DCM) as it diffuses through clay. The first series showed the use of a synthetic leachate with no significant initial bacterial population diffusing through a plug of intact clay; there was an induction period of 95--135 d, during which diffusion was as expected in the absence of degradation, followed by a second stage, where degradation occurred with an apparent half-life of less than 55 d at a temperature of 24 C. The second series of tests examined the diffusion of an actual leachate from the Keele Valley Landfill (KVL) (which provided both nutrients and a source of bacteria), through a compacted clay. In these tests, the induction period was reduced to 40--60 d, after which the apparent half-life was 20 d or less at 27 C. The diffusion coefficient for DCM was approximately 8 {times} 10{sup {minus}10} m{sup 2}/s, with partitioning coefficient K{sub d} = ...

British Library Electronic Table of Contents (United Kingdom)

Background The objective of this study was to confirm provisional quantitative trait loci (QTL) for cocaine-induced locomotor activation, on chromosomes 1, 5, 6, 9, 12, 15, 16, 17, and 18, previously identified in the AXB/BXA recombinant inbred (RI) and AcB/BcA recombinant congenic (RC) strains of mice derived from A/J (A) and C57BL/6J (B6) progenitors. This was accomplished through a genetic analysis of cocaine-induced activity in an AxB6 F2 cross and a phenotypic survey across a panel of B6.A chromosome substitution strains (CSS) mice. Mice were tested for cocaine-induced activity, following administration of saline and cocaine (20?mg/kg), utilizing an open-field procedure. Results Among AxB6 F2 mice, differences in cocaine-induced activity were associated with loci on chromosome 1 (D1Mi...

Energy Technology Data Exchange (ETDEWEB)

In this work a study of simulation of the gamma graphic studies that are carried out in human esophagus in the Dept. of Nuclear Medicine of the 'Celestino Hernandez Robau Hospital of Santa Clara is presented. For the investigation tubular reactors were used and sting-response techniques with radioactive tracer of Technetium 99 metastable to a concentration of 1 mCi and several flows were applied. The distribution curves of residences times were obtained, those that respond to an equation of the type: Y = A + B exp (- exp((x-C)/D)) - ((x-C/D)+1). They were also carried out, optimizations studies of the doses of the radioactive to give to the patients from 1 mCi (that is the one used in studies) up to 0,5 mCi, and the influences on the obtained distributions of residence time were analyzed. It was confirmed the possibility to lower the doses with clear information of the signal. It was also carried out a simulation of the attenuation of ...

International Nuclear Information System (INIS)

The accurate staging of rectal carcinoma is very important for treatment planning. The histological data obtained from surgical specimens of 22 patients with rectal carcinoma were compared with pre-and postoperative endorectal US findings and with preoperative CT results. According to an adapted version of Astler and Coller classification, the different degrees of tumor spread into the rectal wall were represented as follows: stage A: 1 patient; stage B1: 5 patients; stage B2: 6 patients; stage C1: 1 patient; stage C2: 8 patients and stage D: 1 patient. Preoperative staging, based on the overall results of CT and US, was in agreement with histology in 19 of 22 cases. Individual analysis of US and CT results, in comparison with histological data, showed US staging accuracy to be 77.3% (17/22 patients). US accuracy in demonstrating tumor spread into the rectal wall (stages A, B1, C1) was 100% (7/7 patients); US was 70% accurate in lymph node ...

International Nuclear Information System (INIS)

We have applied photofragment ion imaging to investigate the dissociation dynamics of low-lying, doubly excited states of molecular hydrogen. A doubly excited electronic state is one in which both of the hydrogen electrons reside in excited molecular orbitals. Two-step, two-color multiphoton excitation of H_2, first via 201.8 nm, two-photon excitation into the E, F "1#SIGMA#"+_g(v_E=0, J=1) state, followed by #approx#563 nm, 1+m (m=1, 2) excitation through the B double-prime "1#SIGMA#"+_u(v=0, J=0, 2), D "1#PI#_u(v=2, J=1, 2), and B' "1#SIGMA#"+_u(v=4, J=0, 2) states provides a ready means of populating several low-lying doubly excited states of H_2 at increasing internuclear separations. From these doubly excited repulsive states, both dissociation and autoionization processes are possible. Because the excitation energy remains relatively constant as each intermediate state is accessed, differences in the photodissociation dynamics via each ...

Energy Technology Data Exchange (ETDEWEB)

This document is the third annual revision of the plans and schedules for implementing the Federal Facility Agreement (FFA) compliance program, originally submitted in 1992 as ES/ER-17&D1, Federal Facility Agreement Plans and Schedules for Liquid Low-Level Radioactive Waste Tank Systems at Oak Ridge National Laboratory, Oak Ridge, Tennessee. This document summarizes the progress that has been made to date in implementing the plans and schedules for meeting the FFA commitments for the Liquid Low-Level Waste (LLLW) System at Oak Ridge National Laboratory (ORNL). Information presented in this document provides a comprehensive summary to facilitate understanding of the FFA compliance program for LLLW tank systems and to present plans and schedules associated with remediation, through the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) process, of LLLW tank systems that have been removed from service. ORNL has a ...

International Nuclear Information System (INIS)

The autors examined the hypothesis that current recommendations on dietary energy requirements may underestimate the total energy needs of young adult men, by measuring total energy expenditure (TEE) and resting energy expenditure (REE) in 14 weight-maintaining healthy subjects leading unrestricted lives. TEE and body composition were measured by using 2H(2)18O, and REE was measured by using indirect calorimetry. All subjects had sedentary full-time occupations and participated in strenuous leisure activities for 34 #+-# 6 (SE) min/d. TEE and REE were 14.61 #+-# 0.76 and 7.39 #+-# 0.26 MJ/d, respectively, and 202 #+-# 2 and 122 #+-# 2 kJ.kg-1.d-1. There were significant relationships between TEE and both body fat-free mass (r = 0.732, P less than 0.005) and measured REE (r = 0.568, P less than 0.05). Measured TEE:REE values were significantly higher than the recommended energy requirement (1.98 #+-# 0.09, compared with 1.55 or 1.67, P less than ...

International Nuclear Information System (INIS)

It has been previously stated that the minimum physiological recommendations for the indispensable amino acids in health adults, as proposed by FAO/WHO/UNU in 1985, are far too low, except for the methionine. An amino acid stable isotopic kinetic study was conducted to seek further experimental support to this hypothesis. Twenty healthy young men received an l-amino acid based diet, supplying 140 mg N.kg"-"1.d"-"1, patterned on egg protein for 1 week, then for 3 weeks either i) a pattern based on current international recommendations (FAO diet, n=7), ii) a the tentative Laboratory of Human Nutrition of the Massachusetts Institute of Technology, new amino acid recommendation pattern (MIT diet, n=7) or iii) again the egg hen pattern (EGG diet, n=6). All subjects were again studied for one final, consecutive week of the egg diet. At the end of the initial week, at the first and third week with the three experimental diets,and after three days ...

International Nuclear Information System (INIS)

This paper provides information on heat transfer enhancement due to jet mixing inside a cylindrical enclosure. The work addresses conservative heat transfer assumptions regarding mixing and condensation that have typically been incorporated into passive containment design analyses. The current research presents an interesting possibility for increasing decay heat removal of passive containment systems under combined natural and forced convection. Eliminating these conservative assumptions could provide the basis for a change of containment design and reduce the construction cost. It is found that the ratio of forced- and free convection Nusselt numbers can be predicted as a function of the Archimedes number and a correlated factor accounting for jet orientation and enclosure geometry. To use the small-scale tests for large containment design, scale-up methods and criteria are important for matching the key governing parameters and fluid properties. In the present experiment, a ...