Atomistic insight into the catalytic mechanism of glycosyltransferases by combined quantum mechanics/molecular mechanics (QM/MM) methods.

Science.gov (United States)

Tvaroška, Igor

2015-02-11

Glycosyltransferases catalyze the formation of glycosidic bonds by assisting the transfer of a sugar residue from donors to specific acceptor molecules. Although structural and kinetic data have provided insight into mechanistic strategies employed by these enzymes, molecular modeling studies are essential for the understanding of glycosyltransferase catalyzed reactions at the atomistic level. For such modeling, combined quantum mechanics/molecular mechanics (QM/MM) methods have emerged as crucial. These methods allow the modeling of enzymatic reactions by using quantum mechanical methods for the calculation of the electronic structure of the active site models and treating the remaining enzyme environment by faster molecular mechanics methods. Herein, the application of QM/MM methods to glycosyltransferase catalyzed reactions is reviewed, and the insight from modeling of glycosyl transfer into the mechanisms and transition states structures of both inverting and retaining glycosyltransferases are discussed. Copyright © 2014 Elsevier Ltd. All rights reserved.

Surviving historical Patagonian landscapes and climate: molecular insights from Galaxias maculatus

Directory of Open Access Journals (Sweden)

Carrea Cecilia

2010-03-01

Full Text Available Abstract Background The dynamic geological and climatic histories of temperate South America have played important roles in shaping the contemporary distributions and genetic diversity of endemic freshwater species. We use mitochondria and nuclear sequence variation to investigate the consequences of mountain barriers and Quaternary glacial cycles for patterns of genetic diversity in the diadromous fish Galaxias maculatus in Patagonia (~300 individuals from 36 locations. Results Contemporary populations of G. maculatus, east and west of the Andes in Patagonia, represent a single monophyletic lineage comprising several well supported groups. Mantel tests using control region data revealed a strong positive relationship when geographic distance was modeled according to a scenario of marine dispersal. (r = 0.69, P = 0.055. By contrast, direct distance between regions was poorly correlated with genetic distance (r = -0.05, P = 0.463. Hierarchical AMOVAs using mtDNA revealed that pooling samples according to historical (pre-LGM oceanic drainage (Pacific vs. Atlantic explained approximately four times more variance than pooling them into present-day drainage (15.6% vs. 3.7%. Further post-hoc AMOVA tests revealed additional genetic structure between populations east and west of the Chilean Coastal Cordillera (coastal vs. interior. Overall female effective population size appears to have remained relatively constant until roughly 0.5 Ma when population size rapidly increased several orders of magnitude [100× (60×-190×] to reach contemporary levels. Maximum likelihood analysis of nuclear alleles revealed a poorly supported gene tree which was paraphyletic with respect to mitochondrial-defined haplogroups. Conclusions First diversifying in the central/north-west region of Patagonia, G. maculatus extended its range into Argentina via the southern coastal regions that join the Pacific and Atlantic oceans. More recent gene flow between northern

Molecular basis of Bcl-X(L-p53 interaction: insights from molecular dynamics simulations.

Directory of Open Access Journals (Sweden)

Nagakumar Bharatham

Full Text Available Bcl-X(L, an antiapoptotic Bcl-2 family protein, plays a central role in the regulation of the apoptotic pathway. Heterodimerization of the antiapoptotic Bcl-2 family proteins with the proapoptotic family members such as Bad, Bak, Bim and Bid is a crucial step in the apoptotic regulation. In addition to these conventional binding partners, recent evidences reveal that the Bcl-2 family proteins also interact with noncanonical binding partners such as p53. Our previous NMR studies showed that Bcl-X(L: BH3 peptide and Bcl-X(L: SN15 peptide (a peptide derived from residues S15-N29 of p53 complex structures share similar modes of bindings. To further elucidate the molecular basis of the interactions, here we have employed molecular dynamics simulations coupled with MM/PBSA approach. Bcl-X(L and other Bcl-2 family proteins have 4 hydrophobic pockets (p1-p4, which are occupied by four systematically spaced hydrophobic residues (h1-h4 of the proapoptotic Bad and Bak BH3 peptides. We observed that three conserved hydrophobic residues (F19, W23 and L26 of p53 (SN15 peptide anchor into three hydrophobic pockets (p2-p4 of Bcl-X(L in a similar manner as BH3 peptide. Our results provide insights into the novel molecular recognition by Bcl-X(L with p53.

Paleo-dust insights onto dust-climate interactions

Science.gov (United States)

Albani, S.; Mahowald, N. M.

2017-12-01

Mineral dust emissions are affected by changing climate conditions, and in turn dust impacts the atmospheric radiation budget, clouds and biogeochemical cycles. Climate and public health dust-related issues call for attention on the fate of the dust cycle in the future, and the representation of the dust cycle is now part of the strategy of the Paleoclimate Modelling Intercomparison Project phase 4 and the Coupled Model Intercomparison Project phase 6 (PMIP4-CMIP6). Since mineral aerosols are one of the most important natural aerosols, understanding past dust responses to climate in the paleoclimate will allow us to better understand mineral aerosol feedbacks with climate and biogeochemistry in the Anthropocene. Modern observations and paleoclimate records offer the possibility of multiple, complementary views on the global dust cycle, and allow to validate and/or constrain the numerical representation of dust in climate and Earth system models. We present our results from a set of simulations with the Community Earth System Model for different climate states, including present and past climates such as the pre-industrial, the mid-Holocene and the Last Glacial Maximum. A set of simulations including a prognostic dust cycle was thoroughly compared with a wide set of present day observations from different platforms and regions, in order to realistically constrain the magnitude of dust load, surface concentration, deposition, optical properties, and particle size distributions. The magnitude of emissions for past climate regimes was constrained based on compilations of paleodust mass accumulation rates and size distributions, as well as based on information on dust provenance. The comparison with a parallel set of simulations without dust allows estimating the impacts of dust on surface climate. We analyze impacts of dust on the mean and variability of surface temperature and precipitation in each climate state, as well as the impacts that changing dust emissions had

Molecular-level insights into aging processes of skin elastin

DEFF Research Database (Denmark)

Mora Huertas, Angela C; Schmelzer, Christian E H; Hoehenwarter, Wolfgang

2016-01-01

Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating...... the influence of intrinsic (chronological aging) and extrinsic factors (sun exposure) on the morphology and susceptibility of elastin towards enzymatic degradation. Elastin was isolated from biopsies derived from sun-protected or sun-exposed skin of differently aged individuals. The morphology of the elastin...... pronounced in sun-exposed tissue. Marker peptides were identified, which showed an age-related increase or decrease in their abundances and provide insights into the progression of the aging process of elastin fibers. Strong age-related cleavage occurs in hydrophobic tropoelastin domains 18, 20, 24 and 26...

Absolute Molecular Orientation of Isopropanol at Ceria (100) Surfaces: Insight into Catalytic Selectivity from the Interfacial Structure

Energy Technology Data Exchange (ETDEWEB)

Doughty, Benjamin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Goverapet Srinivasan, Sriram [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Indian Inst. of Technology (IIT), Rajasthan (India); Bryantsev, Vyacheslav S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Dongkyu [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Ho Nyung [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ma, Ying-Zhong [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lutterman, Daniel A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

2017-06-12

The initial mechanistic steps underlying heterogeneous chemical catalysis can be described in a framework where the composition, structure, and orientation of molecules adsorbed to reactive interfaces are known. However, extracting this vital information is the limiting step in most cases due in part to challenges in probing the interfacial monolayer with enough chemical specificity to characterize the surface molecular constituents. These challenges are exacerbated at complex or spatially heterogeneous interfaces where competing processes and a distribution of local environments can uniquely drive chemistry. To address these limitations, this work presents a distinctive combination of materials synthesis, surface specific optical experiments, and theory to probe and understand molecular structure at catalytic interfaces. Specifically, isopropanol was adsorbed to surfaces of the model CeO₂ catalyst that were synthesized with only the (100) facet exposed. Vibrational sum-frequency generation was used to probe the molecular monolayer, and with the guidance of density functional theory calculations, was used to extract the structure and absolute molecular orientation of isopropanol at the CeO₂ (100) surface. Our results show that isopropanol is readily deprotonated at the surface, and through the measured absolute molecular orientation of isopropanol, we obtain new insight into the selectivity of the (100) surface to form propylene. Our findings reveal key insight into the chemical and physical phenomena taking place at pristine interfaces thereby pointing to intuitive structural arguments to describe catalytic selectivity in more complex systems.

Life on thin ice: Insights from Uummannaq, Greenland for connecting climate science with Arctic communities

Science.gov (United States)

Baztan, Juan; Cordier, Mateo; Huctin, Jean-Michel; Zhu, Zhiwei; Vanderlinden, Jean-Paul

2017-09-01

What are the links between mainstream climate science and local community knowledge? This study takes the example of Greenland, considered one of the regions most impacted by climate change, and Inuit people, characterized as being highly adaptive to environmental change, to explore this question. The study is based on 10 years of anthropological participatory research in Uummannaq, Northwest Greenland, along with two fieldwork periods in October 2014 and April 2015, and a quantitative bibliometric analysis of the international literature on sea ice - a central subject of concern identified by Uummannaq community members during the fieldwork periods. Community members' perceptions of currently available scientific climate knowledge were also collected during the fieldwork. This was done to determine if community members consider available scientific knowledge salient and if it covers issues they consider relevant. The bibliometric analysis of the sea ice literature provided additional insight into the degree to which scientific knowledge about climate change provides information relevant for the community. Our results contribute to the ongoing debate on the missing connections between community worldviews, cultural values, livelihood needs, interests and climate science. Our results show that more scientific research efforts should consider local-level needs in order to produce local-scale knowledge that is more salient, credible and legitimate for communities experiencing climate change. In Uummannaq, as in many Inuit communities with similar conditions, more research should be done on sea ice thickness in winter and in areas through which local populations travel. This paper supports the growing evidence that whenever possible, climate change research should focus on environmental features that matter to communities, at temporal and spatial scales relevant to them, in order to foster community adaptations to change. We recommend such research be connected to and

Adaptation Insights: Lessons from participatory research in Africa ...

International Development Research Centre (IDRC) Digital Library (Canada)

21 avr. 2016 ... The Adaptation Insights series consists of nine case studies from seven projects supported by the Climate Change Adaptation in Africaprogram. Each brief presents insights from research carried out with the active involvement of communities at risk from climate change. The series includes case studies ...

Insights into Penultimate Interglacial-Glacial Climate Change on Vegetation History at Lake Van, Turkey

Science.gov (United States)

Pickarski, N.; Litt, T.

2017-12-01

-resolution record presents an improved insight into regional vegetation dynamics and climate variability in the eastern Mediterranean region.

Molecular records of climate variability and vegetation response since the Late Pleistocene in the Lake Victoria basin, East Africa

NARCIS (Netherlands)

Berke, M.A.; Johnson, T.C.; Werne, J.P.; Grice, K.; Schouten, S.; Sinninghe Damsté, J.S.

2012-01-01

New molecular proxies of temperature and hydrology are helping to constrain tropical climate change and elucidate possible forcing mechanisms during the Holocene. Here, we examine a similar to 14,000 year record of climate variability from Lake Victoria, East Africa, the world's second largest

Milestone in the NTB phase investigation and beyond: direct insight into molecular self-assembly.

Science.gov (United States)

Ivšić, Trpimir; Vinković, Marijana; Baumeister, Ute; Mikleušević, Ana; Lesac, Andreja

2014-12-14

Although liquid-crystalline materials are most widely exploited for flat-panel displays, their ability to self-organize into periodically ordered nanostructures gives rise to a broad variety of additional applications. The recently discovered low-temperature nematic phase (N(TB)) with unusual characteristics generated considerable attention within the scientific community: despite the fact that the molecules from which the phase is composed are not chiral, the helicoidal structure of the phase is strongly implicated. Here we report on combined experimental, computational and spectroscopic studies of the structural aspects influencing formation of the N(TB) phase as well as on the molecular organization within the phase. In an extensive DFT study, the structure-property prerequisite was traced to a "bent-propeller" shape of the molecule. We also demonstrate the first utilization of liquid state NMR for direct analysis of intermolecular interactions within thermotropic liquid-crystalline phases, providing new insight into molecular packing that can lead towards design of novel chiral functional materials. The synergy of experimental, computational and NMR studies suggests a syn-parallel helical molecular organization within the N(TB) phase.

Molecular proxies for climate maladaptation in a long-lived tree (Pinus pinaster Aiton, Pinaceae).

Science.gov (United States)

Jaramillo-Correa, Juan-Pablo; Rodríguez-Quilón, Isabel; Grivet, Delphine; Lepoittevin, Camille; Sebastiani, Federico; Heuertz, Myriam; Garnier-Géré, Pauline H; Alía, Ricardo; Plomion, Christophe; Vendramin, Giovanni G; González-Martínez, Santiago C

2015-03-01

Understanding adaptive genetic responses to climate change is a main challenge for preserving biological diversity. Successful predictive models for climate-driven range shifts of species depend on the integration of information on adaptation, including that derived from genomic studies. Long-lived forest trees can experience substantial environmental change across generations, which results in a much more prominent adaptation lag than in annual species. Here, we show that candidate-gene SNPs (single nucleotide polymorphisms) can be used as predictors of maladaptation to climate in maritime pine (Pinus pinaster Aiton), an outcrossing long-lived keystone tree. A set of 18 SNPs potentially associated with climate, 5 of them involving amino acid-changing variants, were retained after performing logistic regression, latent factor mixed models, and Bayesian analyses of SNP-climate correlations. These relationships identified temperature as an important adaptive driver in maritime pine and highlighted that selective forces are operating differentially in geographically discrete gene pools. The frequency of the locally advantageous alleles at these selected loci was strongly correlated with survival in a common garden under extreme (hot and dry) climate conditions, which suggests that candidate-gene SNPs can be used to forecast the likely destiny of natural forest ecosystems under climate change scenarios. Differential levels of forest decline are anticipated for distinct maritime pine gene pools. Geographically defined molecular proxies for climate adaptation will thus critically enhance the predictive power of range-shift models and help establish mitigation measures for long-lived keystone forest trees in the face of impending climate change. Copyright © 2015 by the Genetics Society of America.

Molecular Sociology: Further Insights from Biological and Environmental Aspects

Directory of Open Access Journals (Sweden)

Ahed Jumah Mahmoud Al-Khatib

2015-11-01

Full Text Available The present study expanded our previous study in which features of molecular sociology were mentioned. In this study, we added the microbial dimensions in which it is thought that religiosity may be impacted by microbes that manipulate brains to create better conditions for their existence. This hypothesis is called “biomeme hypothesis”. We talked about other environmental impacts on human behaviors through three studies in which exposure to lead caused violent behaviors ending with arresting in prisons. By conclusion, the present study has expanded our horizon about interferences on various levels including biological and environmental impacts with our behaviors. Although we are convinced that behavior is a very diverse and complex phenomenon and cannot be understood within certain frame as either biologically or environmentally, but further new insights are possible to participate in better understanding of human behaviors. Many behaviors have their roots in religion, and we showed how religious rituals may be affected by some microbes that make to form a microenvironment within the host for microbial benefits.

Can Payments for Ecosystem Services Contribute to Adaptation to Climate Change? Insights from a Watershed in Kenya

Directory of Open Access Journals (Sweden)

Isabel van de Sand

2014-03-01

Full Text Available Climate change presents new challenges for the management of social-ecological systems and the ecosystem services they provide. Although the instrument of payments for ecosystem services (PES has emerged as a promising tool to safeguard or enhance the provision of ecosystem services (ES, little attention has been paid to the potential role of PES in climate change adaptation. As an external stressor climate change has an impact on the social-ecological system in which PES takes place, including the various actors taking part in the PES scheme. Following a short description of the conceptual link between PES and adaptation to climate change, we provide practical insights into the relationship between PES and adaptation to climate change by presenting results from a case study of a rural watershed in Kenya. Drawing upon the results of a participatory vulnerability assessment among potential ecosystem service providers in Sasumua watershed north of Nairobi, we show that PES can play a role in enhancing adaptation to climate change by influencing certain elements of adaptive capacity and incentivizing adaptation measures. In addition, trade-offs and synergies between proposed measures under PES and adaptation to climate change are identified. Results show that although it may not be possible to establish PES schemes based on water utilities as the sole source of financing, embedding PES in a wider adaptation framework creates an opportunity for the development of watershed PES schemes in Africa and ensures their sustainability. We conclude that there is a need to embed PES in a wider institutional framework and that extra financial resources are needed to foster greater integration between PES and adaptation to climate change. This can be achieved through scaling up PES by bringing in other buyers and additional ecosystem services. PES can achieve important coadaptation benefits, but for more effective adaptation outcomes it needs to be combined

Insights into carbon nanotube and graphene formation mechanisms from molecular simulations: a review

International Nuclear Information System (INIS)

Page, A J; Ding, F; Irle, S; Morokuma, K

2015-01-01

The discovery of carbon nanotubes (CNTs) and graphene over the last two decades has heralded a new era in physics, chemistry and nanotechnology. During this time, intense efforts have been made towards understanding the atomic-scale mechanisms by which these remarkable nanostructures grow. Molecular simulations have made significant contributions in this regard; indeed, they are responsible for many of the key discoveries and advancements towards this goal. Here we review molecular simulations of CNT and graphene growth, and in doing so we highlight the many invaluable insights gained from molecular simulations into these complex nanoscale self-assembly processes. This review highlights an often-overlooked aspect of CNT and graphene formation—that the two processes, although seldom discussed in the same terms, are in fact remarkably similar. Both can be viewed as a 0D → 1D → 2D transformation, which converts carbon atoms (0D) to polyyne chains (1D) to a complete sp 2 -carbon network (2D). The difference in the final structure (CNT or graphene) is determined only by the curvature of the catalyst and the strength of the carbon–metal interaction. We conclude our review by summarizing the present shortcomings of CNT/graphene growth simulations, and future challenges to this important area. (review article)

A multi-layered governance framework for incorporating social science insights into adapting to the health impacts of climate change.

Science.gov (United States)

Bowen, Kathryn J; Ebi, Kristie; Friel, Sharon; McMichael, Anthony J

2013-09-10

Addressing climate change and its associated effects is a multi-dimensional and ongoing challenge. This includes recognizing that climate change will affect the health and wellbeing of all populations over short and longer terms, albeit in varied ways and intensities. That recognition has drawn attention to the need to take adaptive actions to lessen adverse impacts over the next few decades from unavoidable climate change, particularly in developing country settings. A range of sectors is responsible for appropriate adaptive policies and measures to address the health risks of climate change, including health services, water and sanitation, trade, agriculture, disaster management, and development. To broaden the framing of governance and decision-making processes by using innovative methods and assessments to illustrate the multi-sectoral nature of health-related adaptation to climate change. This is a shift from sector-specific to multi-level systems encompassing sectors and actors, across temporal and spatial scales. A review and synthesis of the current knowledge in the areas of health and climate change adaptation governance and decision-making processes. A novel framework is presented that incorporates social science insights into the formulation and implementation of adaptation activities and policies to lessen the health risks posed by climate change. Clarification of the roles that different sectors, organizations, and individuals occupy in relation to the development of health-related adaptation strategies will facilitate the inclusion of health and wellbeing within multi-sector adaptation policies, thereby strengthening the overall set of responses to minimize the adverse health effects of climate change.

A multi-layered governance framework for incorporating social science insights into adapting to the health impacts of climate change

Directory of Open Access Journals (Sweden)

Kathryn J. Bowen

2013-09-01

Full Text Available Background: Addressing climate change and its associated effects is a multi-dimensional and ongoing challenge. This includes recognizing that climate change will affect the health and wellbeing of all populations over short and longer terms, albeit in varied ways and intensities. That recognition has drawn attention to the need to take adaptive actions to lessen adverse impacts over the next few decades from unavoidable climate change, particularly in developing country settings. A range of sectors is responsible for appropriate adaptive policies and measures to address the health risks of climate change, including health services, water and sanitation, trade, agriculture, disaster management, and development. Objectives: To broaden the framing of governance and decision-making processes by using innovative methods and assessments to illustrate the multi-sectoral nature of health-related adaptation to climate change. This is a shift from sector-specific to multi-level systems encompassing sectors and actors, across temporal and spatial scales. Design: A review and synthesis of the current knowledge in the areas of health and climate change adaptation governance and decision-making processes. Results: A novel framework is presented that incorporates social science insights into the formulation and implementation of adaptation activities and policies to lessen the health risks posed by climate change. Conclusion: Clarification of the roles that different sectors, organizations, and individuals occupy in relation to the development of health-related adaptation strategies will facilitate the inclusion of health and wellbeing within multi-sector adaptation policies, thereby strengthening the overall set of responses to minimize the adverse health effects of climate change.

A Best Practices Notebook for Disaster Risk Reduction and Climate Change Adaptation: Guidance and Insights for Policy and Practice from the CATALYST Project

NARCIS (Netherlands)

Hare, M.; Bers, van C.; Mysiak, J.; Calliari, E.; Haque, A.; Warner, K.; Yuzva, K.; Zissener, M.; Jaspers, A.M.J.; Timmerman, J.G.

2014-01-01

This publication, A Best Practices Notebook for Disaster Risk Reduction and Climate Change Adaptation: Guidance and Insights for Policy and Practice from the CATALYST Project is one of two main CATALYST knowledge products that focus on the transformative approaches and measures that can support

Factoring the car-climate challenge: Insights and implications

International Nuclear Information System (INIS)

DeCicco, John M.

2013-01-01

Three approaches commonly are identified for controlling automobile greenhouse gas (GHG) emissions: reducing travel demand, improving vehicle efficiency and using alternatively (non-petroleum) fueled vehicles (AFVs). Similarly, sector emissions are factored by travel distance, vehicle fuel intensity and fuel GHG (“carbon”) intensity. Analyzing these factors using US and China data reveals that for a broad range of conditions, stringent GHG emissions limits for the auto sector imply limits of comparable stringency for fuel carbon intensity. However, carbon intensity is a modeled representation of complex energy supply and use systems rather than a measurable property of fuels themselves. Carefully examining the locations and magnitudes of fuel-related emissions indicates that the proper policy focus is on the sectors that supply fuel rather than the choice of fuels in the auto sector. Therefore, beyond fundamental R and D, policies to commercialize AFVs are not necessarily required for climate protection at present. In addition to managing travel demand and improving vehicle efficiency, the implied policy priority is limiting net GHG impacts in the energy and other natural resource sectors that supply fuels. Future work is needed to develop GHG management protocols for liquid fuel supply systems involving fungible commodities and dynamic global supply chains. - Highlights: • Factor analysis offers insights about ways to reduce automotive GHG emissions. • Improving vehicle efficiency and reducing travel demand remain important. • Reducing net GHG impacts in energy sectors that supply motor fuels is also a priority. • Commercialization of alternatively fueled vehicles is not necessarily required at present

Structural insights into the molecular mechanisms of myasthenia gravis and their therapeutic implications

Energy Technology Data Exchange (ETDEWEB)

Noridomi, Kaori; Watanabe, Go; Hansen, Melissa N.; Han, Gye Won; Chen, Lin (USC)

2017-04-25

The nicotinic acetylcholine receptor (nAChR) is a major target of autoantibodies in myasthenia gravis (MG), an autoimmune disease that causes neuromuscular transmission dysfunction. Despite decades of research, the molecular mechanisms underlying MG have not been fully elucidated. Here, we present the crystal structure of the nAChR α1 subunit bound by the Fab fragment of mAb35, a reference monoclonal antibody that causes experimental MG and competes with ~65% of antibodies from MG patients. Our structures reveal for the first time the detailed molecular interactions between MG antibodies and a core region on nAChR α1. These structures suggest a major nAChR-binding mechanism shared by a large number of MG antibodies and the possibility to treat MG by blocking this binding mechanism. Structure-based modeling also provides insights into antibody-mediated nAChR cross-linking known to cause receptor degradation. Our studies establish a structural basis for further mechanistic studies and therapeutic development of MG.

Climate variability and sustainable food production: Insights from ...

African Journals Online (AJOL)

The past two decades have seen invigorated debates on the causal link between climate variability and food crop production. This study[1] extends the debate further by investigating how climate variability has affected the production of four specific food crops: maize, millet, rice, and groundnuts in north-eastern Ghana.

Assessing surface water flood risk and management strategies under future climate change: Insights from an Agent-Based Model.

Science.gov (United States)

Jenkins, K; Surminski, S; Hall, J; Crick, F

2017-10-01

Climate change and increasing urbanization are projected to result in an increase in surface water flooding and consequential damages in the future. In this paper, we present insights from a novel Agent Based Model (ABM), applied to a London case study of surface water flood risk, designed to assess the interplay between different adaptation options; how risk reduction could be achieved by homeowners and government; and the role of flood insurance and the new flood insurance pool, Flood Re, in the context of climate change. The analysis highlights that while combined investment in property-level flood protection and sustainable urban drainage systems reduce surface water flood risk, the benefits can be outweighed by continued development in high risk areas and the effects of climate change. In our simulations, Flood Re is beneficial in its function to provide affordable insurance, even under climate change. However, the scheme does face increasing financial pressure due to rising surface water flood damages. If the intended transition to risk-based pricing is to take place then a determined and coordinated strategy will be needed to manage flood risk, which utilises insurance incentives, limits new development, and supports resilience measures. Our modelling approach and findings are highly relevant for the ongoing regulatory and political approval process for Flood Re as well as for wider discussions on the potential of insurance schemes to incentivise flood risk management and climate adaptation in the UK and internationally. Copyright © 2017 Elsevier B.V. All rights reserved.

Molecular insight into conformational transmission of human P-glycoprotein

International Nuclear Information System (INIS)

Chang, Shan-Yan; Liu, Fu-Feng; Dong, Xiao-Yan; Sun, Yan

2013-01-01

P-glycoprotein (P-gp), a kind of ATP-binding cassette transporter, can export candidates through a channel at the two transmembrane domains (TMDs) across the cell membranes using the energy released from ATP hydrolysis at the two nucleotide-binding domains (NBDs). Considerable evidence has indicated that human P-gp undergoes large-scale conformational changes to export a wide variety of anti-cancer drugs out of the cancer cells. However, molecular mechanism of the conformational transmission of human P-gp from the NBDs to the TMDs is still unclear. Herein, targeted molecular dynamics simulations were performed to explore the atomic detail of the conformational transmission of human P-gp. It is confirmed that the conformational transition from the inward- to outward-facing is initiated by the movement of the NBDs. It is found that the two NBDs move both on the two directions (x and y). The movement on the x direction leads to the closure of the NBDs, while the movement on the y direction adjusts the conformations of the NBDs to form the correct ATP binding pockets. Six key segments (KSs) protruding from the TMDs to interact with the NBDs are identified. The relative movement of the KSs along the y axis driven by the NBDs can be transmitted through α-helices to the rest of the TMDs, rendering the TMDs to open towards periplasm in the outward-facing conformation. Twenty eight key residue pairs are identified to participate in the interaction network that contributes to the conformational transmission from the NBDs to the TMDs of human P-gp. In addition, 9 key residues in each NBD are also identified. The studies have thus provided clear insight into the conformational transmission from the NBDs to the TMDs in human P-gp

Macroscale patterns in body size of intertidal crustaceans provide insights on climate change effects

Science.gov (United States)

Dugan, Jenifer E.; Hubbard, David M.; Contreras, Heraldo; Duarte, Cristian; Acuña, Emilio; Schoeman, David S.

2017-01-01

Predicting responses of coastal ecosystems to altered sea surface temperatures (SST) associated with global climate change, requires knowledge of demographic responses of individual species. Body size is an excellent metric because it scales strongly with growth and fecundity for many ectotherms. These attributes can underpin demographic as well as community and ecosystem level processes, providing valuable insights for responses of vulnerable coastal ecosystems to changing climate. We investigated contemporary macroscale patterns in body size among widely distributed crustaceans that comprise the majority of intertidal abundance and biomass of sandy beach ecosystems of the eastern Pacific coasts of Chile and California, USA. We focused on ecologically important species representing different tidal zones, trophic guilds and developmental modes, including a high-shore macroalga-consuming talitrid amphipod (Orchestoidea tuberculata), two mid-shore scavenging cirolanid isopods (Excirolana braziliensis and E. hirsuticauda), and a low-shore suspension-feeding hippid crab (Emerita analoga) with an amphitropical distribution. Significant latitudinal patterns in body sizes were observed for all species in Chile (21° - 42°S), with similar but steeper patterns in Emerita analoga, in California (32°- 41°N). Sea surface temperature was a strong predictor of body size (-4% to -35% °C-1) in all species. Beach characteristics were subsidiary predictors of body size. Alterations in ocean temperatures of even a few degrees associated with global climate change are likely to affect body sizes of important intertidal ectotherms, with consequences for population demography, life history, community structure, trophic interactions, food-webs, and indirect effects such as ecosystem function. The consistency of results for body size and temperature across species with different life histories, feeding modes, ecological roles, and microhabitats inhabiting a single widespread coastal

When climate science became climate politics: British media representations of climate change in 1988.

Science.gov (United States)

Jaspal, Rusi; Nerlich, Brigitte

2014-02-01

Climate change has become a pressing environmental concern for scientists, social commentators and politicians. Previous social science research has explored media representations of climate change in various temporal and geographical contexts. Through the lens of Social Representations Theory, this article provides a detailed qualitative thematic analysis of media representations of climate change in the 1988 British broadsheet press, given that this year constitutes an important juncture in this transition of climate change from the domain of science to that of the socio-political sphere. The following themes are outlined: (i) "Climate change: a multi-faceted threat"; (ii) "Collectivisation of threat"; (iii) "Climate change and the attribution of blame"; and (iv) "Speculative solutions to a complex socio-environmental problem." The article provides detailed empirical insights into the "starting-point" for present-day disputes concerning climate change and lays the theoretical foundations for tracking the continuities and discontinuities characterising social representations of climate change in the future.

Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis

KAUST Repository

Casas, Laura

2016-10-17

Sequential hermaphroditism is a unique reproductive strategy among teleosts that is displayed mainly in fish species living in the coral reef environment. The reproductive biology of hermaphrodites has long been intriguing; however, very little is known about the molecular pathways underlying their sex change. Here, we provide the first de novo transcriptome analyses of a hermaphrodite teleost´s undergoing sex change in its natural environment. Our study has examined relative gene expression across multiple groups—rather than just two contrasting conditions— and has allowed us to explore the differential expression patterns throughout the whole process. Our analysis has highlighted the rapid and complex genomic response of the brain associated with sex change, which is subsequently transmitted to the gonads, identifying a large number of candidate genes, some well-known and some novel, involved in the process. The present study provides strong evidence of the importance of the sex steroidogenic machinery during sex change in clownfish, with the aromatase gene playing a central role, both in the brain and the gonad. This work constitutes the first genome-wide study in a social sex-changing species and provides insights into the genetic mechanism governing social sex change and gonadal restructuring in protandrous hermaphrodites.

Insight into the binding interactions of CYP450 aromatase inhibitors with their target enzyme: a combined molecular docking and molecular dynamics study.

Science.gov (United States)

Galeazzi, Roberta; Massaccesi, Luca

2012-03-01

CYP450 aromatase catalyzes the terminal and rate-determining step in estrogen synthesis, the aromatization of androgens, and its inhibition is an efficient approach to treating estrogen-dependent breast cancer. Insight into the molecular basis of the interaction at the catalytic site between CYP450 aromatase inhibitors and the enzyme itself is required in order to design new and more active compounds. Hence, a combined molecular docking-molecular dynamics study was carried out to obtain the structure of the lowest energy association complexes of aromatase with some third-generation aromatase inhibitors (AIs) and with other novel synthesized letrozole-derived compounds which showed high in vitro activity. The results obtained clearly demonstrate the role of the pharmacophore groups present in the azaheterocyclic inhibitors (NSAIs)-namely the triazolic ring and highly functionalized aromatic moieties carrying H-bond donor or acceptor groups. In particular, it was pointed out that all of them can contribute to inhibition activity by interacting with residues of the catalytic cleft, but the amino acids involved are different for each compound, even if they belong to the same class. Furthermore, the azaheterocyclic group strongly coordinates with the Fe(II) of heme cysteinate in the most active NSAI complexes, while it prefers to adopt another orientation in less active ones.

Methods of teaching the physics of climate change in undergraduate physics courses

Science.gov (United States)

Sadler, Michael

2015-04-01

Although anthropogenic climate change is generally accepted in the scientific community, there is considerable skepticism among the general population and, therefore, in undergraduate students of all majors. Students are often asked by their peers, family members, and others, whether they ``believe'' climate change is occurring and what should be done about it (if anything). I will present my experiences and recommendations for teaching the physics of climate change to both physics and non-science majors. For non-science majors, the basic approach is to try to develop an appreciation for the scientific method (particularly peer-reviewed research) in a course on energy and the environment. For physics majors, the pertinent material is normally covered in their undergraduate courses in modern physics and thermodynamics. Nevertheless, it helps to review the basics, e.g. introductory quantum mechanics (discrete energy levels of atomic systems), molecular spectroscopy, and blackbody radiation. I have done this in a separate elective topics course, titled ``Physics of Climate Change,'' to help the students see how their knowledge gives them insight into a topic that is very volatile (socially and politically).

Teaching Climate Social Science and Its Practices: A Two-Pronged Approach to Climate Literacy

Science.gov (United States)

Shwom, R.; Isenhour, C.; McCright, A.; Robinson, J.; Jordan, R.

2014-12-01

The Essential Principles of Climate Science Literacy states that a climate-literate individual can: "understand the essential principles of Earth's climate system, assess scientifically credible information about climate change, communicate about climate and climate change in a meaningful way, and make informed and responsible decisions with regard to actions that may affect climate." We argue that further integration of the social science dimensions of climate change will advance the climate literacy goals of communication and responsible actions. The underlying rationale for this argues: 1) teaching the habits of mind and scientific practices that have synergies across the social and natural sciences can strengthen students ability to understand and assess science in general and that 2) understanding the empirical research on the social, political, and economic processes (including climate science itself) that are part of the climate system is an important step for enabling effective action and communication. For example, while climate literacy has often identified the public's faulty mental models of climate processes as a partial explanation of complacency, emerging research suggests that the public's mental models of the social world are equally or more important in leading to informed and responsible climate decisions. Building student's ability to think across the social and natural sciences by understanding "how we know what we know" through the sciences and a scientific understanding of the social world allows us to achieve climate literacy goals more systematically and completely. To enable this integration we first identify the robust social science insights for the climate science literacy principles that involve social systems. We then briefly identify significant social science contributions to climate science literacy that do not clearly fit within the seven climate literacy principles but arguably could advance climate literacy goals. We conclude

Adaptation to climate change in urban areas: climate-greening London, Rotterdam, and Toronto

NARCIS (Netherlands)

Mees, H.L.P.; Driessen, P.P.J.

2011-01-01

This article aims to gain insight into the governance capacity of cities to adapt to climate change through urban green planning, which we will refer to as climate-greening. The use of green space is considered a no-regrets adaptation strategy, since it not only absorbs rainfall and

The Psychology of Climate Change Communication - Insights from the Center for Research on Environmental Decisions (CRED) (Invited)

Science.gov (United States)

Marx, S.

2010-12-01

social goals in favor or self interest; early involvement of stakeholders through participatory processes can help identify key concerns and information needs which can then be addressed in a tailored approach; taking advantage of default effects can make it easier for people to choose environmentally and socially beneficial options. Using research into the reactions of groups as disparate as African farmers and conservative U.S. voters, we offer insights on how scientists, educators, journalists and others can effectively connect with wider audiences. The communication principles presented in this talk can be applied beyond climate change and to science communication in general.

Flood risk and climate change in the Rotterdam area, The Netherlands : enhancing citizen's climate risk perceptions and prevention responses despite skepticism

NARCIS (Netherlands)

de Boer, Joop; Botzen, W. J Wouter; Terpstra, Teun

2016-01-01

Effective communication about climate change and related risks is complicated by the polarization between “climate alarmists” and “skeptics.” This paper provides insights for the design of climate risk communication strategies by examining how the interplay between climate change and flood risk

Flood risk and climate change in the Rotterdam area, The Netherlands: Enhancing citizen's climate risk perceptions and prevention responses despite skepticism

NARCIS (Netherlands)

de Boer, J.; Botzen, W.J.W.; Terpstra, T.

2016-01-01

Effective communication about climate change and related risks is complicated by the polarization between “climate alarmists” and “skeptics.” This paper provides insights for the design of climate risk communication strategies by examining how the interplay between climate change and flood risk

High-throughput molecular analysis in lung cancer: insights into biology and potential clinical applications.

Science.gov (United States)

Ocak, S; Sos, M L; Thomas, R K; Massion, P P

2009-08-01

During the last decade, high-throughput technologies including genomic, epigenomic, transcriptomic and proteomic have been applied to further our understanding of the molecular pathogenesis of this heterogeneous disease, and to develop strategies that aim to improve the management of patients with lung cancer. Ultimately, these approaches should lead to sensitive, specific and noninvasive methods for early diagnosis, and facilitate the prediction of response to therapy and outcome, as well as the identification of potential novel therapeutic targets. Genomic studies were the first to move this field forward by providing novel insights into the molecular biology of lung cancer and by generating candidate biomarkers of disease progression. Lung carcinogenesis is driven by genetic and epigenetic alterations that cause aberrant gene function; however, the challenge remains to pinpoint the key regulatory control mechanisms and to distinguish driver from passenger alterations that may have a small but additive effect on cancer development. Epigenetic regulation by DNA methylation and histone modifications modulate chromatin structure and, in turn, either activate or silence gene expression. Proteomic approaches critically complement these molecular studies, as the phenotype of a cancer cell is determined by proteins and cannot be predicted by genomics or transcriptomics alone. The present article focuses on the technological platforms available and some proposed clinical applications. We illustrate herein how the "-omics" have revolutionised our approach to lung cancer biology and hold promise for personalised management of lung cancer.

A Social Identity Analysis of Climate Change and Environmental Attitudes and Behaviors: Insights and Opportunities.

Science.gov (United States)

Fielding, Kelly S; Hornsey, Matthew J

2016-01-01

Environmental challenges are often marked by an intergroup dimension. Political conservatives and progressives are divided on their beliefs about climate change, farmers come into conflict with scientists and environmentalists over water allocation or species protection, and communities oppose big business and mining companies that threaten their local environment. These intergroup tensions are reminders of the powerful influence social contexts and group memberships can have on attitudes, beliefs, and actions relating to climate change and the environment more broadly. In this paper, we use social identity theory to help describe and explain these processes. We review literature showing, how conceiving of oneself in terms of a particular social identity influences our environmental attitudes and behaviors, how relations between groups can impact on environmental outcomes, and how the content of social identities can direct group members to act in more or less pro-environmental ways. We discuss the similarities and differences between the social identity approach to these phenomena and related theories, such as cultural cognition theory, the theory of planned behavior, and value-belief-norm theory. Importantly, we also advance social-identity based strategies to foster more sustainable environmental attitudes and behaviors. Although this theoretical approach can provide important insights and potential solutions, more research is needed to build the empirical base, especially in relation to testing social identity solutions.

A Social Identity Analysis of Climate Change and Environmental Attitudes and Behaviors: Insights and Opportunities

Science.gov (United States)

Fielding, Kelly S.; Hornsey, Matthew J.

2016-01-01

Environmental challenges are often marked by an intergroup dimension. Political conservatives and progressives are divided on their beliefs about climate change, farmers come into conflict with scientists and environmentalists over water allocation or species protection, and communities oppose big business and mining companies that threaten their local environment. These intergroup tensions are reminders of the powerful influence social contexts and group memberships can have on attitudes, beliefs, and actions relating to climate change and the environment more broadly. In this paper, we use social identity theory to help describe and explain these processes. We review literature showing, how conceiving of oneself in terms of a particular social identity influences our environmental attitudes and behaviors, how relations between groups can impact on environmental outcomes, and how the content of social identities can direct group members to act in more or less pro-environmental ways. We discuss the similarities and differences between the social identity approach to these phenomena and related theories, such as cultural cognition theory, the theory of planned behavior, and value-belief-norm theory. Importantly, we also advance social-identity based strategies to foster more sustainable environmental attitudes and behaviors. Although this theoretical approach can provide important insights and potential solutions, more research is needed to build the empirical base, especially in relation to testing social identity solutions. PMID:26903924

A social identity analysis of climate change and environmental attitudes and behaviors: Insights and opportunities

Directory of Open Access Journals (Sweden)

Kelly Shanene Fielding

2016-02-01

Full Text Available Environmental challenges are often marked by an intergroup dimension. Political conservatives and progressives are divided on their beliefs about climate change, farmers come into conflict with scientists and environmentalists over water allocation or species protection, and communities oppose big business and mining companies that threaten their local environment. These intergroup tensions are reminders of the powerful influence social contexts and group memberships can have on attitudes, beliefs, and actions relating to climate change and the environment more broadly. In this paper we use social identity theory to help describe and explain these processes. We review literature showing how conceiving of oneself in terms of a particular social identity influences our environmental attitudes and behaviors, how relations between groups can impact on environmental outcomes, and how the content of social identities can direct group members to act in more or less pro-environmental ways. We discuss the similarities and differences between the social identity approach to these phenomena and related theories such as cultural cognition theory, the theory of planned behavior and value-belief-norm theory. Importantly, we also advance social-identity based strategies to foster more sustainable environmental attitudes and behaviors. Although this theoretical approach can provide important insights and potential solutions, more research is needed to build the empirical base, especially in relation to testing social identity solutions.

Calcium-Activated Cl- Channel: Insights on the Molecular Identity in Epithelial Tissues.

Science.gov (United States)

Rottgen, Trey S; Nickerson, Andrew J; Rajendran, Vazhaikkurichi M

2018-05-10

Calcium-activated chloride secretion in epithelial tissues has been described for many years. However, the molecular identity of the channel responsible for the Ca 2+ -activated Cl − secretion in epithelial tissues has remained a mystery. More recently, TMEM16A has been identified as a new putative Ca 2+ -activated Cl − channel (CaCC). The primary goal of this article will be to review the characterization of TMEM16A, as it relates to the physical structure of the channel, as well as important residues that confer voltage and Ca 2+ -sensitivity of the channel. This review will also discuss the role of TMEM16A in epithelial physiology and potential associated-pathophysiology. This will include discussion of developed knockout models that have provided much needed insight on the functional localization of TMEM16A in several epithelial tissues. Finally, this review will examine the implications of the identification of TMEM16A as it pertains to potential novel therapies in several pathologies.

Natural and molecular history of prolactinoma: insights from a Prlr-/- mouse model.

Science.gov (United States)

Bernard, Valérie; Villa, Chiara; Auguste, Aurélie; Lamothe, Sophie; Guillou, Anne; Martin, Agnès; Caburet, Sandrine; Young, Jacques; Veitia, Reiner A; Binart, Nadine

2018-01-19

Lactotroph adenoma, also called prolactinoma, is the most common pituitary tumor but little is known about its pathogenesis. Mouse models of prolactinoma can be useful to better understand molecular mechanisms involved in abnormal lactotroph cell proliferation and secretion. We have previously developed a prolactin receptor deficient ( Prlr -/- ) mouse, which develops prolactinoma. The present study aims to explore the natural history of prolactinoma formation in Prlr -/- mice, using hormonal, radiological, histological and molecular analyses to uncover mechanisms involved in lactotroph adenoma development. Prlr -/- females develop large secreting prolactinomas from 12 months of age, with a penetrance of 100%, mimicking human aggressive densely granulated macroprolactinoma, which is a highly secreting subtype. Mean blood PRL measurements reach 14 902 ng/mL at 24 months in Prlr -/- females while PRL levels were below 15 ng/mL in control mice ( p model in ACI rats, we pinpointed 218 concordantly differentially expressed (DE) genes involved in cell cycle, mitosis, cell adhesion molecules, dopaminergic synapse and estrogen signaling. Pathway/gene-set enrichment analyses suggest that the transcriptomic dysregulation in both models of prolactinoma might be mediated by a limited set of transcription factors (i.e., STAT5, STAT3, AhR, ESR1, BRD4, CEBPD, YAP, FOXO1) and kinases (i.e., JAK2, AKT1, BRAF, BMPR1A, CDK8, HUNK, ALK, FGFR1, ILK). Our experimental results and their bioinformatic analysis provide insights into early genomic changes in murine models of the most frequent human pituitary tumor.

The economics of climate change and the change of climate in economics

International Nuclear Information System (INIS)

Marechal, Kevin

2007-01-01

Economics is an unavoidable decision-making tool in the field of climate policy. At the same time, traditional economics is being challenged both empirically and theoretically by scholars in different fields. Its non-neutrality in dealing with climate-related issues-which is illustrated by the controversy over the 'no-regret potential'-would thus call for an opening of economics to insights from other disciplines. Within that context, we show that an evolutionary-inspired line of thought coupled with a systemic and historical perspective of technological change provides a very insightful alternative to traditional economics. More particularly, it follows from that framework that the picture of the climate challenge ahead looks very different from what traditional economic analyses would suggest. For instance, the lock-in process makes it unlikely that traditional cost-efficient measures (such as carbon taxation or tradable emission rights) will be sufficient to bring about the required radical changes in the field of energy as they fail to address structural barriers highlighted in our approach

Hydroclimate variability in Scandinavia over the last millennium - insights from a climate model-proxy data comparison

Science.gov (United States)

Seftigen, Kristina; Goosse, Hugues; Klein, Francois; Chen, Deliang

2017-12-01

The integration of climate proxy information with general circulation model (GCM) results offers considerable potential for deriving greater understanding of the mechanisms underlying climate variability, as well as unique opportunities for out-of-sample evaluations of model performance. In this study, we combine insights from a new tree-ring hydroclimate reconstruction from Scandinavia with projections from a suite of forced transient simulations of the last millennium and historical intervals from the CMIP5 and PMIP3 archives. Model simulations and proxy reconstruction data are found to broadly agree on the modes of atmospheric variability that produce droughts-pluvials in the region. Despite these dynamical similarities, large differences between simulated and reconstructed hydroclimate time series remain. We find that the GCM-simulated multi-decadal and/or longer hydroclimate variability is systematically smaller than the proxy-based estimates, whereas the dominance of GCM-simulated high-frequency components of variability is not reflected in the proxy record. Furthermore, the paleoclimate evidence indicates in-phase coherencies between regional hydroclimate and temperature on decadal timescales, i.e., sustained wet periods have often been concurrent with warm periods and vice versa. The CMIP5-PMIP3 archive suggests, however, out-of-phase coherencies between the two variables in the last millennium. The lack of adequate understanding of mechanisms linking temperature and moisture supply on longer timescales has serious implications for attribution and prediction of regional hydroclimate changes. Our findings stress the need for further paleoclimate data-model intercomparison efforts to expand our understanding of the dynamics of hydroclimate variability and change, to enhance our ability to evaluate climate models, and to provide a more comprehensive view of future drought and pluvial risks.

Trichoderma for climate resilient agriculture.

Science.gov (United States)

Kashyap, Prem Lal; Rai, Pallavi; Srivastava, Alok Kumar; Kumar, Sudheer

2017-08-01

Climate change is one of the biggest challenges of the twenty-first century for sustainable agricultural production. Several reports highlighted the need for better agricultural practices and use of eco-friendly methods for sustainable crop production under such situations. In this context, Trichoderma species could be a model fungus to sustain crop productivity. Currently, these are widely used as inoculants for biocontrol, biofertilization, and phytostimulation. They are reported to improve photosynthetic efficiency, enhance nutrient uptake and increase nitrogen use efficiency in crops. Moreover, they can be used to produce bio-energy, facilitate plants for adaptation and mitigate adverse effect of climate change. The technological advancement in high throughput DNA sequencing and biotechnology provided deep insight into the complex and diverse biotic interactions established in nature by Trichoderma spp. and efforts are being made to translate this knowledge to enhance crop growth, resistance to disease and tolerance to abiotic stresses under field conditions. The discovery of several traits and genes that are involved in the beneficial effects of Trichoderma spp. has resulted in better understanding of the performance of bioinoculants in the field, and will lead to more efficient use of these strains and possibly to their improvement by genetic modification. The present mini-review is an effort to elucidate the molecular basis of plant growth promotion and defence activation by Trichoderma spp. to garner broad perspectives regarding their functioning and applicability for climate resilient agriculture.

Insight to forcing of late Quaternary climate change from aeolian dust archives in eastern Australia

Science.gov (United States)

McGowan, H. A.; Marx, S.; Soderholm, J.; Denholm, J.; Petherick, L.

2010-12-01

The Australian continent is the largest source of dust in the Southern Hemisphere. Historical dust emissions records display inter-annual variability in response to the El Niño Southern Oscillation (ENSO) phenomenon and inter-decadal variability which has been linked to the Pacific Decadal Oscillation (PDO). These reflect change in hydrometeorology of the continents two major dust source regions, the Murray-Darling Basin and the Lake Eyre Basin. The historical records do not allow longer term variability of ENSO and the PDO and their influence on Australia to be quantified. Importantly, sub-Milankovitch centennial to multi-millennial scale climate cycles and their impacts are not represented in the historical records. In this paper we present summary results from the analysis of two aeolain dust records spanning 7 ka and 45 ka. These were developed from ombrotrophic mire and lacustrine sediment cores collected from the Australian Alps and southeast Queensland. Both sites are located in the southeast Australian dust transport pathway and provide rare insight to forcings of climate variability and its impacts on eastern Australia through the late Quaternary. Age controls for the cores were established using 14C and 210Pb dating [McGowan et al. 2008, 2010]. The cores were sliced into 2 to 5 mm segments with a sub-sample of each segment combusted at 450°C for 12 hrs to destroy organic material and allow recovery of mineral dust. Geochemical fingerprinting of the global climate variability and the impact of forcings originating from the North Hemisphere. These results highlight the potential for adverse impacts on the climate of Australia by disturbance to North Atlantic Ocean circulation. References Marx, S. K., et al. 2005: Provenance of long travelled dust determined with ultra trace element composition: A pilot study with samples from New Zealand glaciers. Earth Surf. Processes Landforms, 30, 699-716. McGowan, H.A., et al. 2008: An ultra-high resolution record of

Insights into the immuno-molecular biology of Angiostrongylus vasorum through transcriptomics--prospects for new interventions.

Science.gov (United States)

Ansell, Brendan R E; Schnyder, Manuela; Deplazes, Peter; Korhonen, Pasi K; Young, Neil D; Hall, Ross S; Mangiola, Stefano; Boag, Peter R; Hofmann, Andreas; Sternberg, Paul W; Jex, Aaron R; Gasser, Robin B

2013-12-01

Angiostrongylus vasorum is a metastrongyloid nematode of dogs and other canids of major clinical importance in many countries. In order to gain first insights into the molecular biology of this worm, we conducted the first large-scale exploration of its transcriptome, and predicted essential molecules linked to metabolic and biological processes as well as host immune responses. We also predicted and prioritized drug targets and drug candidates. Following Illumina sequencing (RNA-seq), 52.3 million sequence reads representing adult A. vasorum were assembled and annotated. The assembly yielded 20,033 contigs, which encoded proteins with 11,505 homologues in Caenorhabditis elegans, and additional 2252 homologues in various other parasitic helminths for which curated data sets were publicly available. Functional annotation was achieved for 11,752 (58.6%) proteins predicted for A. vasorum, including peptidases (4.5%) and peptidase inhibitors (1.6%), protein kinases (1.7%), G protein-coupled receptors (GPCRs) (1.5%) and phosphatases (1.2%). Contigs encoding excretory/secretory and immuno-modulatory proteins represented some of the most highly transcribed molecules, and encoded enzymes that digest haemoglobin were conserved between A. vasorum and other blood-feeding nematodes. Using an essentiality-based approach, drug targets, including neurotransmitter receptors, an important chemosensory ion channel and cysteine proteinase-3 were predicted in A. vasorum, as were associated small molecular inhibitors/activators. Future transcriptomic analyses of all developmental stages of A. vasorum should facilitate deep explorations of the molecular biology of this important parasitic nematode and support the sequencing of its genome. These advances will provide a foundation for exploring immuno-molecular aspects of angiostrongylosis and have the potential to underpin the discovery of new methods of intervention. © 2013.

The terrestrial hydro-climate of the Early Eocene: insights from the oxygen and clumped isotope composition of pedogenic siderite

Science.gov (United States)

van Dijk, J.; Fernandez, A.; Müller, I.; White, T. S.; Bernasconi, S. M.

2016-12-01

The Early Eocene (56 Ma) is the youngest period of Earth's history when CO2 concentrations in the atmosphere (600-1500 ppm) reached levels close to those predicted for future emission scenarios. Proxy-based climate reconstructions from this interval can therefore be used to gain insights on effects that anthropogenic emissions might have on the climate system. So far, Early Eocene climatic data is limited to the oceans, where proxies for temperature are abundant and relatively well understood. However, in order to get a complete picture of the Early Eocene climate, temperature and rainfall reconstructions on the continental paleo-surface are needed. Here, we present clumped and stable oxygen isotope measurements of siderite samples collected along a North-South transect in the North American Continent. These siderites formed in kaolinitic soils that developed globally under the extremely wet and warm conditions of the Early Eocene. They provide a record of both soil temperature and the δ18O composition of meteoric water, which can be used to unravel the regional paleo-precipitation rate. Both parameters were estimated using an elaborate in-house calibration constructed with synthetic siderite precipitated in the presence or absence of iron reducing bacteria. Measurements of δD on plant-derived N-alkanes present within the same soils align well with our δ18Owater data, confirming an Early Eocene meteoric water line similar to the present day. We provide an estimate of the meridional temperature gradient during the Early Eocene and offer constraints on the boundary conditions of the Earth's hydrologic cycle under high pCO2.

Insight derived from molecular dynamics simulations into molecular motions, thermodynamics and kinetics of HIV-1 gp120.

Directory of Open Access Journals (Sweden)

Peng Sang

Full Text Available Although the crystal structures of the HIV-1 gp120 core bound and pre-bound by CD4 are known, the details of dynamics involved in conformational equilibrium and transition in relation to gp120 function have remained elusive. The homology models of gp120 comprising the N- and C-termini and loops V3 and V4 in the CD4-bound and CD4-unbound states were built and subjected to molecular dynamics (MD simulations to investigate the differences in dynamic properties and molecular motions between them. The results indicate that the CD4-bound gp120 adopted a more compact and stable conformation than the unbound form during simulations. For both the unbound and bound gp120, the large concerted motions derived from essential dynamics (ED analyses can influence the size/shape of the ligand-binding channel/cavity of gp120 and, therefore, were related to its functional properties. The differences in motion direction between certain structural components of these two forms of gp120 were related to the conformational interconversion between them. The free energy calculations based on the metadynamics simulations reveal a more rugged and complex free energy landscape (FEL for the unbound than for the bound gp120, implying that gp120 has a richer conformational diversity in the unbound form. The estimated free energy difference of ∼-6.0 kJ/mol between the global minimum free energy states of the unbound and bound gp120 indicates that gp120 can transform spontaneously from the unbound to bound states, revealing that the bound state represents a high-probability "ground state" for gp120 and explaining why the unbound state resists crystallization. Our results provide insight into the dynamics-and-function relationship of gp120, and facilitate understandings of the thermodynamics, kinetics and conformational control mechanism of HIV-1 gp120.

Climate for change

International Nuclear Information System (INIS)

Newell, P.

2000-01-01

Climate for Change: Non-State Actors and the Global Politics of the Greenhouse provides a challenging explanation of the forces that have shaped the international global warming debate. Unlike existing books on the politics of climate change, this book concentrates on how non-stage actors, such as scientific, environmental and industry groups, as opposed to governmental organisations, affect political outcomes in global fora on climate change. It also provides insights in to the role of the media in influencing the agenda. The book draws on a range of analytical approaches to assess and explain the influence of these non-governmental organisations in the course of global climate change politics. The book will be of interest to all researchers and policy-makers associated with climate change, and will be used on university courses in international relations, politics and environmental studies. (Author)

Climate protection in practice: how municipalities master their ambitious climate protection goals; Klimaschutz konkret. Wie meistern Kommunen ihre anspruchsvollen Klimaschutzziele?

Energy Technology Data Exchange (ETDEWEB)

Moser, Peter [dE Institut dezentrale Energietechnologien gGmbH Europaeische Geschaeftsstelle, Kassel (Germany)

2014-07-01

This paper offers insights into the multifarious challenges facing municipalities in the field of municipal climate protection. It outlines typical local situations and explains the most important features of municipal climate protection: its specific goals and concepts and climate protection management and networking. It pinpoints success criteria and challenges and closes with some critical reflection.

An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food

Directory of Open Access Journals (Sweden)

Jodi Woan-Fei Law

2015-11-01

Full Text Available Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria Enrichment Broth (BLEB, Fraser broth and University of Vermont Medium (UVM Listeria enrichment broth are recommended by regulatory agencies such as FDA-BAM, USDA-FSIS and ISO. Many plating media are available for the isolation of L. monocytogenes, for instance, PALCAM, Oxford and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. MPN technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction (PCR, multiplex polymerase chain reaction (mPCR, real-time/quantitative polymerase chain reaction (qPCR, nucleic acid sequence-based amplification (NASBA, loop-mediated isothermal amplification (LAMP, DNA microarray and Next Generation Sequencing (NGS technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labour-saving. In future, there are chances for the development of new techniques for the detection and identification of foodborne with improved features.

Assessing Climate Risk on Agricultural Production: Insights Using Retrospective Analysis of Crop Insurance and Climatic Trends

Science.gov (United States)

Reyes, J. J.; Elias, E.; Eischens, A.; Shilts, M.; Rango, A.; Steele, R.

2017-12-01

The collaborative synthesis of existing datasets, such as long-term climate observations and farmers' crop insurance payments, can increase their overall collective value and societal application. The U.S. Department of Agriculture (USDA) Climate Hubs were created to develop and deliver science-based information and technologies to agricultural and natural resource managers to enable climate-informed decision-making. As part of this mission, Hubs work across USDA and other climate service agencies to synthesize existing information. The USDA Risk Management Agency (RMA) is responsible for overseeing the Federal crop insurance program which currently insures over $100 billion in crops annually. RMA hosts data describing the cause for loss (e.g. drought, wind, irrigation failure) and indemnity amount (i.e. total cost of loss) at multiple spatio-temporal scales (i.e. state, county, year, month). The objective of this paper is to link climate information with indemnities, and their associated cause of loss, to assess climate risk on agricultural production and provide regionally-relevant information to stakeholders to promote resilient working landscapes. We performed a retrospective trend analysis at the state-level for the American Southwest (SW). First, we assessed indemnity-only trends by cause of loss and crop type at varying temporal scales. Historical monthly weather data (i.e. precipitation and temperature) and long-term drought indices (e.g. Palmer Drought Severity Index) were then linked with indemnities and grouped by different causes of loss. Climatological ranks were used to integrate historical comparative intensity of acute and long-term climatic events. Heat and drought as causes of loss were most correlated with temperature and drought indicators, respectively. Across all SW states increasing indemnities were correlated with warmer conditions. Multiple statistical trend analyses suggest a framework is necessary to appropriately measure the biophysical

Characterization of hairless (Hr) and FGF5 genes provides insights into the molecular basis of hair loss in cetaceans.

Science.gov (United States)

Chen, Zhuo; Wang, Zhengfei; Xu, Shixia; Zhou, Kaiya; Yang, Guang

2013-02-09

Hair is one of the main distinguishing characteristics of mammals and it has many important biological functions. Cetaceans originated from terrestrial mammals and they have evolved a series of adaptations to aquatic environments, which are of evolutionary significance. However, the molecular mechanisms underlying their aquatic adaptations have not been well explored. This study provided insights into the evolution of hair loss during the transition from land to water by investigating and comparing two essential regulators of hair follicle development and hair follicle cycling, i.e., the Hairless (Hr) and FGF5 genes, in representative cetaceans and their terrestrial relatives. The full open reading frame sequences of the Hr and FGF5 genes were characterized in seven cetaceans. The sequence characteristics and evolutionary analyses suggested the functional loss of the Hr gene in cetaceans, which supports the loss of hair during their full adaptation to aquatic habitats. By contrast, positive selection for the FGF5 gene was found in cetaceans where a series of positively selected amino acid residues were identified. This is the first study to investigate the molecular basis of the hair loss in cetaceans. Our investigation of Hr and FGF5, two indispensable regulators of the hair cycle, provide some new insights into the molecular basis of hair loss in cetaceans. The results suggest that positive selection for the FGF5 gene might have promoted the termination of hair growth and early entry into the catagen stage of hair follicle cycling. Consequently, the hair follicle cycle was disrupted and the hair was lost completely due to the loss of the Hr gene function in cetaceans. This suggests that cetaceans have evolved an effective and complex mechanism for hair loss.

The fluid dynamics of climate

CERN Document Server

Palazzi, Elisa; Fraedrich, Klaus

2016-01-01

This volume provides an overview of the fluid aspects of the climate system, focusing on basic aspects as well as recent research developments. It will bring together contributions from diverse fields of the physical, mathematical and engineering sciences. The volume will be useful to doctorate students, postdocs and researchers working on different aspects of atmospheric, oceanic and environmental fluid dynamics. It will also be of interest to researchers interested in quantitatively understanding how fluid dynamics can be applied to the climate system, and to climate scientists willing to gain a deeper insight into the fluid mechanics underlying climate processes.

New Insights into Molecular Organization of Human Neuraminidase-1: Transmembrane Topology and Dimerization Ability

Science.gov (United States)

Maurice, Pascal; Baud, Stéphanie; Bocharova, Olga V.; Bocharov, Eduard V.; Kuznetsov, Andrey S.; Kawecki, Charlotte; Bocquet, Olivier; Romier, Beatrice; Gorisse, Laetitia; Ghirardi, Maxime; Duca, Laurent; Blaise, Sébastien; Martiny, Laurent; Dauchez, Manuel; Efremov, Roman G.; Debelle, Laurent

2016-12-01

Neuraminidase 1 (NEU1) is a lysosomal sialidase catalyzing the removal of terminal sialic acids from sialyloconjugates. A plasma membrane-bound NEU1 modulating a plethora of receptors by desialylation, has been consistently documented from the last ten years. Despite a growing interest of the scientific community to NEU1, its membrane organization is not understood and current structural and biochemical data cannot account for such membrane localization. By combining molecular biology and biochemical analyses with structural biophysics and computational approaches, we identified here two regions in human NEU1 - segments 139-159 (TM1) and 316-333 (TM2) - as potential transmembrane (TM) domains. In membrane mimicking environments, the corresponding peptides form stable α-helices and TM2 is suited for self-association. This was confirmed with full-size NEU1 by co-immunoprecipitations from membrane preparations and split-ubiquitin yeast two hybrids. The TM2 region was shown to be critical for dimerization since introduction of point mutations within TM2 leads to disruption of NEU1 dimerization and decrease of sialidase activity in membrane. In conclusion, these results bring new insights in the molecular organization of membrane-bound NEU1 and demonstrate, for the first time, the presence of two potential TM domains that may anchor NEU1 in the membrane, control its dimerization and sialidase activity.

Limitations of climatic data for inferring species boundaries: insights from speckled rattlesnakes.

Directory of Open Access Journals (Sweden)

Jesse M Meik

Full Text Available Phenotypes, DNA, and measures of ecological differences are widely used in species delimitation. Although rarely defined in such studies, ecological divergence is almost always approximated using multivariate climatic data associated with sets of specimens (i.e., the "climatic niche"; the justification for this approach is that species-specific climatic envelopes act as surrogates for physiological tolerances. Using identical statistical procedures, we evaluated the usefulness and validity of the climate-as-proxy assumption by comparing performance of genetic (nDNA SNPs and mitochondrial DNA, phenotypic, and climatic data for objective species delimitation in the speckled rattlesnake (Crotalus mitchellii complex. Ordination and clustering patterns were largely congruent among intrinsic (heritable traits (nDNA, mtDNA, phenotype, and discordance is explained by biological processes (e.g., ontogeny, hybridization. In contrast, climatic data did not produce biologically meaningful clusters that were congruent with any intrinsic dataset, but rather corresponded to regional differences in atmospheric circulation and climate, indicating an absence of inherent taxonomic signal in these data. Surrogating climate for physiological tolerances adds artificial weight to evidence of species boundaries, as these data are irrelevant for that purpose. Based on the evidence from congruent clustering of intrinsic datasets, we recommend that three subspecies of C. mitchellii be recognized as species: C. angelensis, C. mitchellii, and C. Pyrrhus.

Gene expression profiles give insight into the molecular pathology of bone in primary hyperparathyroidism

DEFF Research Database (Denmark)

Reppe, Sjur; Stilgren, Lis; Olstad, Ole K

2006-01-01

to bone and extra-cellular matrix, showed altered expression. Of these were 85 up- and 14 down-regulated before operation. The majority of regulated genes represented structural and adhesion proteins, but included also proteases and protease regulators which promote resorption. Increased expressions...... of collagen type 1 and osteocalcin mRNAs in disease reflecting the PTH anabolic action were paralleled by increased concentrations of these proteins in serum. In addition, genes encoding transcriptional factors and their regulators as well as cellular signal molecules were up-regulated during disease....... The identified genetic signature represents the first extensive description of the ensemble of bone and matrix related mRNAs, which are regulated by chronic PTH action. These results identify the molecular basis for this skeletal disease, and provide new insight into this clinical condition with potential...

Molecular Electronics: Insight from First-Principles Transport Simulations

DEFF Research Database (Denmark)

Paulsson, Magnus; Frederiksen, Thomas; Brandbyge, Mads

2010-01-01

Conduction properties of nanoscale contacts can be studied using first-principles simulations. Such calculations give insight into details behind the conductance that is not readily available in experiments. For example, we may learn how the bonding conditions of a molecule to the electrodes affect...

Using Morphological, Molecular and Climatic Data to Delimitate Yews along the Hindu Kush-Himalaya and Adjacent Regions

Science.gov (United States)

Poudel, Ram C.; Möller, Michael; Gao, Lian-Ming; Ahrends, Antje; Baral, Sushim R.; Liu, Jie; Thomas, Philip; Li, De-Zhu

2012-01-01

Background Despite the availability of several studies to clarify taxonomic problems on the highly threatened yews of the Hindu Kush-Himalaya (HKH) and adjacent regions, the total number of species and their exact distribution ranges remains controversial. We explored the use of comprehensive sets of morphological, molecular and climatic data to clarify taxonomy and distributions of yews in this region. Methodology/Principal Findings A total of 743 samples from 46 populations of wild yew and 47 representative herbarium specimens were analyzed. Principle component analyses on 27 morphological characters and 15 bioclimatic variables plus altitude and maximum parsimony analysis on molecular ITS and trnL-F sequences indicated the existence of three distinct species occurring in different ecological (climatic) and altitudinal gradients along the HKH and adjacent regions Taxus contorta from eastern Afghanistan to the eastern end of Central Nepal, T. wallichiana from the western end of Central Nepal to Northwest China, and the first report of the South China low to mid-elevation species T. mairei in Nepal, Bhutan, Northeast India, Myanmar and South Vietnam. Conclusion/Significance The detailed sampling and combination of different data sets allowed us to identify three clearly delineated species and their precise distribution ranges in the HKH and adjacent regions, which showed no overlap or no distinct hybrid zone. This might be due to differences in the ecological (climatic) requirements of the species. The analyses further provided the selection of diagnostic morphological characters for the identification of yews occurring in the HKH and adjacent regions. Our work demonstrates that extensive sampling combined with the analysis of diverse data sets can reliably address the taxonomy of morphologically challenging plant taxa. PMID:23056501

Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

KAUST Repository

Dineshram, Ramadoss

2016-03-19

The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. © 2016 John Wiley & Sons Ltd.

Molecular-level insights into aging processes of skin elastin.

Science.gov (United States)

Mora Huertas, Angela C; Schmelzer, Christian E H; Hoehenwarter, Wolfgang; Heyroth, Frank; Heinz, Andrea

2016-01-01

Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating the influence of intrinsic (chronological aging) and extrinsic factors (sun exposure) on the morphology and susceptibility of elastin towards enzymatic degradation. Elastin was isolated from biopsies derived from sun-protected or sun-exposed skin of differently aged individuals. The morphology of the elastin fibers was characterized by scanning electron microscopy. Mass spectrometric analysis and label-free quantification allowed identifying differences in the cleavage patterns of the elastin samples after enzymatic digestion. Principal component analysis and hierarchical cluster analysis were used to visualize differences between the samples and to determine the contribution of extrinsic and intrinsic aging to the proteolytic susceptibility of elastin. Moreover, the release of potentially bioactive peptides was studied. Skin aging is associated with the decomposition of elastin fibers, which is more pronounced in sun-exposed tissue. Marker peptides were identified, which showed an age-related increase or decrease in their abundances and provide insights into the progression of the aging process of elastin fibers. Strong age-related cleavage occurs in hydrophobic tropoelastin domains 18, 20, 24 and 26. Photoaging makes the N-terminal and central parts of the tropoelastin molecules more susceptible towards enzymatic cleavage and, hence, accelerates the age-related degradation of elastin. Copyright © 2016 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food

Science.gov (United States)

Law, Jodi Woan-Fei; Ab Mutalib, Nurul-Syakima; Chan, Kok-Gan; Lee, Learn-Han

2015-01-01

Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration, and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria enrichment broth, Fraser broth, and University of Vermont Medium (UVM) Listeria enrichment broth are recommended by regulatory agencies such as Food and Drug Administration-bacteriological and analytical method (FDA-BAM), US Department of Agriculture-Food and Safety (USDA-FSIS), and International Organization for Standardization (ISO). Many plating media are available for the isolation of L. monocytogenes, for instance, polymyxin acriflavin lithium-chloride ceftazidime aesculin mannitol, Oxford, and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method, and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. most probable number technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction, multiplex polymerase chain reaction, real-time/quantitative polymerase chain reaction, nucleic acid sequence-based amplification, loop-mediated isothermal amplification, DNA microarray, and next generation sequencing technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labor-saving. In future, there are

Global Climate Change as Environmental Megacrisis

OpenAIRE

Endter-Wada, Joanna; Ingram, Helen

2012-01-01

The authors analyze global climate change utilizing insights from the governance and crisis management literatures that seek to understand the prospects, nature, characteristics and the effects of cataclysmic events. They argue that global climate change is a mega-crisis hiding in plain sight yet there has been no proportionate mega-crisis response. People are still grappling with how to make sense of climate change, how to bridge multiple ways of knowing it, and how to negotiate collective c...

Climate variability and sustainable food production: Insights from ...

African Journals Online (AJOL)

They are integrated and balance the ... implement resilient agricultural practices that increase productivity and production; that help maintain ecosystems ... other forms of life, the manner in which human beings respond to climate variability is critical not ..... work for longer hours and at the same time its effect on their health.

Flood risk and climate change in the Rotterdam area, The Netherlands: Enhancing citizen's climate risk perceptions and prevention responses despite skepticism

OpenAIRE

de Boer, J.; Botzen, W.J.W.; Terpstra, T.

2016-01-01

Effective communication about climate change and related risks is complicated by the polarization between “climate alarmists” and “skeptics.” This paper provides insights for the design of climate risk communication strategies by examining how the interplay between climate change and flood risk communication affects citizens’ risk perceptions and responses. The study is situated in a delta area with substantial geographic variations in the occurrence and potential impact of flood risk, which ...

Interactive Correlation Analysis and Visualization of Climate Data

Energy Technology Data Exchange (ETDEWEB)

Ma, Kwan-Liu [Univ. of California, Davis, CA (United States)

2016-09-21

The relationship between our ability to analyze and extract insights from visualization of climate model output and the capability of the available resources to make those visualizations has reached a crisis point. The large volume of data currently produced by climate models is overwhelming the current, decades-old visualization workflow. The traditional methods for visualizing climate output also have not kept pace with changes in the types of grids used, the number of variables involved, and the number of different simulations performed with a climate model or the feature-richness of high-resolution simulations. This project has developed new and faster methods for visualization in order to get the most knowledge out of the new generation of high-resolution climate models. While traditional climate images will continue to be useful, there is need for new approaches to visualization and analysis of climate data if we are to gain all the insights available in ultra-large data sets produced by high-resolution model output and ensemble integrations of climate models such as those produced for the Coupled Model Intercomparison Project. Towards that end, we have developed new visualization techniques for performing correlation analysis. We have also introduced highly scalable, parallel rendering methods for visualizing large-scale 3D data. This project was done jointly with climate scientists and visualization researchers at Argonne National Laboratory and NCAR.

Insights into regioselective metabolism of mefenamic acid by cytochrome P450 BM3 mutants through crystallography, docking, molecular dynamics, and free energy calculations

DEFF Research Database (Denmark)

Capoferri, Luigi; Leth, Rasmus; Ter Haar, Ernst

2016-01-01

of the protein mutant M11 was expressed, purified, and crystallized, and its X-ray structure was used as template for modeling. A multistep approach was used that combines molecular docking, molecular dynamics (MD) simulation, and binding free-energy calculations to address protein flexibility. In this way...... active-site mutations such as V87I were reported to invert regioselectivity in NSAID hydroxylation. In this work, we combine crystallography and molecular simulation to study the effect of single mutations on binding and regioselective metabolism of mefenamic acid by M11 mutants. The heme domain...... of mefenamic acid by M11 and its mutants by including protein flexibility and dynamics in free-energy computation. In addition, we could obtain structural insights into the change in regioselectivity of mefenamic acid hydroxylation due to single active-site mutations. Our findings confirm that use of MD...

Molecular Insights into Crimean-Congo Hemorrhagic Fever Virus

Directory of Open Access Journals (Sweden)

Marko Zivcec

2016-04-01

Full Text Available Crimean-Congo hemorrhagic fever virus (CCHFV is a tick-borne pathogen that causes high morbidity and mortality. Efficacy of vaccines and antivirals to treat human CCHFV infections remains limited and controversial. Research into pathology and underlying molecular mechanisms of CCHFV and other nairoviruses is limited. Significant progress has been made in our understanding of CCHFV replication and pathogenesis in the past decade. Here we review the most recent molecular advances in CCHFV-related research, and provide perspectives on future research.

Crystal Nucleation in Liquids: Open Questions and Future Challenges in Molecular Dynamics Simulations.

Science.gov (United States)

Sosso, Gabriele C; Chen, Ji; Cox, Stephen J; Fitzner, Martin; Pedevilla, Philipp; Zen, Andrea; Michaelides, Angelos

2016-06-22

The nucleation of crystals in liquids is one of nature's most ubiquitous phenomena, playing an important role in areas such as climate change and the production of drugs. As the early stages of nucleation involve exceedingly small time and length scales, atomistic computer simulations can provide unique insights into the microscopic aspects of crystallization. In this review, we take stock of the numerous molecular dynamics simulations that, in the past few decades, have unraveled crucial aspects of crystal nucleation in liquids. We put into context the theoretical framework of classical nucleation theory and the state-of-the-art computational methods by reviewing simulations of such processes as ice nucleation and the crystallization of molecules in solutions. We shall see that molecular dynamics simulations have provided key insights into diverse nucleation scenarios, ranging from colloidal particles to natural gas hydrates, and that, as a result, the general applicability of classical nucleation theory has been repeatedly called into question. We have attempted to identify the most pressing open questions in the field. We believe that, by improving (i) existing interatomic potentials and (ii) currently available enhanced sampling methods, the community can move toward accurate investigations of realistic systems of practical interest, thus bringing simulations a step closer to experiments.

Climate adaptation

Science.gov (United States)

Kinzig, Ann P.

2015-03-01

This paper is intended as a brief introduction to climate adaptation in a conference devoted otherwise to the physics of sustainable energy. Whereas mitigation involves measures to reduce the probability of a potential event, such as climate change, adaptation refers to actions that lessen the impact of climate change. Mitigation and adaptation differ in other ways as well. Adaptation does not necessarily have to be implemented immediately to be effective; it only needs to be in place before the threat arrives. Also, adaptation does not necessarily require global, coordinated action; many effective adaptation actions can be local. Some urban communities, because of land-use change and the urban heat-island effect, currently face changes similar to some expected under climate change, such as changes in water availability, heat-related morbidity, or changes in disease patterns. Concern over those impacts might motivate the implementation of measures that would also help in climate adaptation, despite skepticism among some policy makers about anthropogenic global warming. Studies of ancient civilizations in the southwestern US lends some insight into factors that may or may not be important to successful adaptation.

Inspiring your audience to action: insights from theory and practice

Science.gov (United States)

Wong, W.; Hekkers, J.; Mott, B.

2011-12-01

Findings from market research sponsored by The Ocean Project, along with many other recent studies, have revealed two troubling facts: 1. Despite increased efforts to grow climate and ocean literacy among the general public, American adult's knowledge of climate and ocean health has remained stagnant in the past decade; and 2. Knowledge and level of concern about climate change show little correlation, i.e. the people who are most concerned about climate change are not the ones who know most about the science of climate change, and vice versa. If knowledge does not lead to action among the general public, what implications does this have for those of us working for conservation? How can we motivate people to act for conservation? The Ocean Project's large-scale survey of American attitudes and values vis-à-vis ocean, climate change, and related conservation issues provides answers to many such burning questions. Our research findings reveal critical insights about what, who, and how we can communicate for maximum efficacy. In particular, youth and minorities emerged as important constituencies: not only are they more environmentally aware and/or socially conscious, they are important influencers who demonstrate greater propensity to modify their behaviors and/or engage in conservation advocacy. Our presentation will discuss the implications of these findings for strategic communication for conservation action as well as present case studies from the Monterey Bay Aquarium that support these research findings and provide insights from evaluation of two significantly different interpretive approaches to communicate about climate change-a live animal exhibit and a video-based, live-narrated auditorium program.

Molecular photoionization dynamics

International Nuclear Information System (INIS)

Dehmer, J.L.

1982-01-01

This program seeks to develop both physical insight and quantitative characterization of molecular photoionization processes. Progress is briefly described, and some publications resulting from the research are listed

The Social Impact of Climate

Science.gov (United States)

Hsiang, S. M.

2013-12-01

Managing climate change requires that we understand the social value of climate-related decisions. Rational decision-making demands that we weigh the potential benefits of climate-related investments against their costs. To date, it has been challenging to quantify the relative social benefit of living under different climatic conditions, so policy debates tend to focus on investment costs without considering their benefits. Here I will discuss challenges and advances in the measurement of climate's impact on society. By linking data and methods across physical and social sciences, we are beginning to understand when, where, and how climatic conditions have a causal impact on human wellbeing. I will present examples from this burgeoning interdisciplinary field that quantify the effect of temperature on macroeconomic performance, the effects of climate on human conflict, and the long-term health and economic impact of tropical cyclones. Each of these examples provide new insight into previously unknown benefits of various climate management strategies. I conclude by describing new efforts to systematically gather and compare findings from across the research community to support informed and rational climate management decisions.

Evaluating climate change mitigation potential of hydrochars: compounding insights from three different indicators

DEFF Research Database (Denmark)

Owsianiak, Mikołaj; Brooks, Jennifer; Renz, Michael

2017-01-01

beet, fava bean, onion and lucerne) and two different countries (Spain and Germany), and used three different indicators of climate change: global warming potential (GWP), global temperature change potential (GTP), and climate tipping potential (CTP). We found that although climate change benefits (GWP......) from just sequestration and temporary storage of carbon are sufficient to outweigh impacts stemming from hydrochar production and transportation to the field, even greater benefits stem from replacing climate-inefficient biowaste management treatment options, like composting in Spain. By contrast...

Making Sustainable Energy Choices: Insights on the Energy/Water/Land Nexus

Energy Technology Data Exchange (ETDEWEB)

2014-10-01

This periodic publication summarizes insights from the body of NREL analysis work. In this issue of Analysis Insights, we examine the implications of our energy choices on water, land use, climate, developmental goals, and other factors. Collectively, NREL's work helps policymakers and investors understand and evaluate energy choices within the complex web of connections, or nexus, between energy, water, and land.

Climate program "stone soup": Assessing climate change vulnerabilities in the Aleutian and Bering Sea Islands of Alaska

Science.gov (United States)

Littell, J. S.; Poe, A.; van Pelt, T.

2015-12-01

Climate change is already affecting the Bering Sea and Aleutian Island region of Alaska. Past and present marine research across a broad spectrum of disciplines is shedding light on what sectors of the ecosystem and the human dimension will be most impacted. In a grassroots approach to extend existing research efforts, leveraging recently completed downscaled climate projections for the Bering Sea and Aleutian Islands region, we convened a team of 30 researchers-- with expertise ranging from anthropology to zooplankton to marine mammals-- to assess climate projections in the context of their expertise. This Aleutian-Bering Climate Vulnerability Assessment (ABCVA) began with researchers working in five teams to evaluate the vulnerabilities of key species and ecosystem services relative to projected changes in climate. Each team identified initial vulnerabilities for their focal species or services, and made recommendations for further research and information needs that would help managers and communities better understand the implications of the changing climate in this region. Those draft recommendations were shared during two focused, public sessions held within two hub communities for the Bering and Aleutian region: Unalaska and St. Paul. Qualitative insights about local concerns and observations relative to climate change were collected during these sessions, to be compared to the recommendations being made by the ABCVA team of researchers. Finally, we used a Structured Decision Making process to prioritize the recommendations of participating scientists, and integrate the insights shared during our community sessions. This work brought together residents, stakeholders, scientists, and natural resource managers to collaboratively identify priorities for addressing current and expected future impacts of climate change. Recommendations from this project will be incorporated into future research efforts of the Aleutian and Bering Sea Islands Landscape Conservation

Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis

KAUST Repository

Casas, Laura; Saborido-Rey, Fran; Ryu, Tae Woo; Michell, Craig; Ravasi, Timothy; Irigoien, Xabier

2016-01-01

steroidogenic machinery during sex change in clownfish, with the aromatase gene playing a central role, both in the brain and the gonad. This work constitutes the first genome-wide study in a social sex-changing species and provides insights into the genetic

Assessing customer satisfaction for improving NOAA's climate products and services

Science.gov (United States)

Meyers, J. C.; Hawkins, M. D.; Timofeyeva, M. M.

2009-12-01

NOAA's National Weather Service (NWS) Climate Services Division (CSD) is developing a comprehensive climate user requirements process with the ultimate goal of producing climate services that meet the needs of NWS climate information users. An important part of this effort includes engaging users through periodical surveys conducted by the Claes Fornell International (CFI) Group using the American Customer Satisfaction Index (ACSI). The CFI Group conducted a Climate Services Satisfaction (CSS) Survey in May of 2009 to measure customer satisfaction with current products and services and to gain insight on areas for improvement. The CSS Survey rates customer satisfaction on a range of NWS climate services data and products, including Climate Prediction Center (CPC) outlooks, drought monitoring, and ENSO monitoring and forecasts, as well as NWS local climate data services. In addition, the survey assesses the users of the products to give the NWS insight into its climate customer base. The survey also addresses specific topics such as NWS forecast category names, probabilistic nature of climate products, and interpretation issues. The survey results identify user requirements for improving existing NWS climate services and introducing new ones. CSD will merge the survey recommendations with available scientific methodologies and operational capabilities to develop requirements for improved climate products and services. An overview of the 2009 survey results will be presented, such as users' satisfaction with the accuracy, reliability, display and functionality of products and services.

Insights into Meteoric 10Be Dynamics and Climate Stability along the Hawaiian Kohala Climosequence

Science.gov (United States)

Dixon, J. L.; Chadwick, O.

2017-12-01

We measure meteoric 10Be in soils across a well-studied climate gradient spanning Kohala, Hawaii to provide new understanding of the isotope behavior in soils and constraints on nuclide delivery rates to Earth's surface. Annual rainfall across the Kohala climogradient varies from 16 - 300 cm, with Hawaiian soils reflecting evolution over the past 150 ka, the nominal age of the volcanic parent material. We analyzed a sequence of nine soil profiles for meteoric 10Be and compared with previously measured data on soil chemistry and dust fluxes. In the Kohala system, soil inventories of 10Be span 40-300 x 109 atom/cm2 and generally increase linearly with rainfall, consistent with precipitation-driven fluxes and the high retention of 10Be in clay-rich soil horizons. However, nuclide inventories dramatically decrease for soils at rainfall >140 cm/y. The observed decrease corresponds with other strong changes in weathering intensity across the climate gradient, associated with previously studied and recognized pedogenic thresholds. These thresholds represent abrupt transitions in soil chemistry related to increased throughflow of soil solutions, decreases in base saturation and pH, and the destruction of phyllosilicates and replacement with amorphous oxyhydroxides. Meteoric-derived ages, based on 10Be-flux estimates and measured inventories are uniform for dry soils ( 60ka), but far less than the known substrate age (150ka), indicating that actual delivery rates are lower than predicted from current models in this region. Despite the offset in predicted and substrate ages, the consistency in pattern suggests that the rainfall gradient over the 150 thousand years of soil development has not deviated significantly from its present structure. Furthermore, based on clear 10Be losses in soils with high moisture availability, our results indicate meteoric 10Be may not be a robust tracer of soil age and movement in systems with high rainfall and weathering intensity and low soil

Molecular Insights into the Transmembrane Domain of the Thyrotropin Receptor.

Directory of Open Access Journals (Sweden)

Vanessa Chantreau

Full Text Available The thyrotropin receptor (TSHR is a G protein-coupled receptor (GPCR that is member of the leucine-rich repeat subfamily (LGR. In the absence of crystal structure, the success of rational design of ligands targeting the receptor internal cavity depends on the quality of the TSHR models built. In this subfamily, transmembrane helices (TM 2 and 5 are characterized by the absence of proline compared to most receptors, raising the question of the structural conformation of these helices. To gain insight into the structural properties of these helices, we carried out bioinformatics and experimental studies. Evolutionary analysis of the LGR family revealed a deletion in TM5 but provided no information on TM2. Wild type residues at positions 2.58, 2.59 or 2.60 in TM2 and/or at position 5.50 in TM5 were substituted to proline. Depending on the position of the proline substitution, different effects were observed on membrane expression, glycosylation, constitutive cAMP activity and responses to thyrotropin. Only proline substitution at position 2.59 maintained complex glycosylation and high membrane expression, supporting occurrence of a bulged TM2. The TSHR transmembrane domain was modeled by homology with the orexin 2 receptor, using a protocol that forced the deletion of one residue in the TM5 bulge of the template. The stability of the model was assessed by molecular dynamics simulations. TM5 straightened during the equilibration phase and was stable for the remainder of the simulations. Our data support a structural model of the TSHR transmembrane domain with a bulged TM2 and a straight TM5 that is specific of glycoprotein hormone receptors.

Molecular fossils in modern genomes provide physiological and geochemical insights to the ancient earth (Invited)

Science.gov (United States)

Dupont, C.; Caetano-Anolles, G.

2010-12-01

The genomes of extant organisms are ultimately derived from ancient life, thus theoretically contain insight to ancient physiology, ecology, and environments. In particular, metalloenzymes may be particularly insightful. The fundamental chemistry of trace elements dictates the molecular speciation and reactivity both within cells and the environment at large. Using protein structure and comparative genomics, we elucidate several major influences this chemistry has had upon biology. All of life exhibits the same proteome size-dependent scaling for the number of metal-binding proteins within a proteome. This fundamental evolutionary constant shows that the selection of one element occurs at the exclusion of another, with the eschewal of Fe for Zn and Ca being a defining feature of eukaryotic pro- teomes. Early life lacked both the structures required to control intracellular metal concentrations and the metal-binding proteins that catalyze electron transport and redox transformations. The development of protein structures for metal homeostasis coincided with the emergence of metal-specific structures, which predomi- nantly bound metals abundant in the Archean ocean. Potentially, this promoted the diversification of emerging lineages of Archaea and Bacteria through the establishment of biogeochemical cycles. In contrast, structures binding Cu and Zn evolved much later, pro- viding further evidence that environmental availability influenced the selection of the elements. The late evolving Zn-binding proteins are fundamental to eukaryotic cellular biology, and Zn bioavailabil- ity may have been a limiting factor in eukaryotic evolution. The results presented here provide an evolutionary timeline based on genomic characteristics, and key hypotheses can be tested by alternative geochemical methods.

Translating Scientific Judgment, Technological Insight and Economic Theory Into Practical Policy Lessons: The Case of Climate Regulation in the United States

Science.gov (United States)

Mignone, B. K.

2008-12-01

Effective solutions to the climate change problem will require unprecedented cooperation across space, continuity across time and coordination between disciplines. One well-known methodology for synthesizing the lessons of physical science, energy engineering and economics is integrated assessment. Typically, integrated assessment models use scientific and technological relationships as physical constraints in a larger macroeconomic optimization that is designed to either balance the costs and benefits of climate change mitigation or find the least-cost path to an exogenously prescribed endpoint (e.g. atmospheric CO2 stabilization). The usefulness of these models depends to a large extent on the quality of the assumptions and the relevance of the outcome metrics chosen by the user. In this study, I show how a scientifically-based emissions reduction scenario can be combined with engineering-based assumptions about the energy system (e.g. estimates of the marginal cost premium of carbon-free technology) to yield insights about the price path of CO2 under a future regulatory regime. I then show how this outcome metric (carbon price) relates to key decisions about the design of a future cap-and-trade system and the way in which future carbon markets may be regulated.

1.2 million years of climate change, globally and in the Mediterranean

NARCIS (Netherlands)

Konijnendijk, T.Y.M.

2015-01-01

In this thesis we make a detailed reconstruction of climate changes based on materials from the Mediterranean Sea. Not only does this provide new insights in climate changes in the Mediterranean region, the aim is to improve our understanding of global climate changes as well. We created a single

Using Data from Climate Science to Teach Introductory Statistics

Science.gov (United States)

Witt, Gary

2013-01-01

This paper shows how the application of simple statistical methods can reveal to students important insights from climate data. While the popular press is filled with contradictory opinions about climate science, teachers can encourage students to use introductory-level statistics to analyze data for themselves on this important issue in public…

What the Framework Convention on Climate Change Teaches Us About Cooperation on Climate Change

Directory of Open Access Journals (Sweden)

David G. Victor

2016-09-01

Full Text Available Arild Underdal has been at the center of an important community of scholars studying global environmental governance. Since the 1990s that community, along with many other scholars globally, has offered important insights into the design and management of international institutions that can lead to more effective management of environmental problems. At the same time, diplomats have made multiple attempts to create institutions to manage the dangers of climate change. This essay looks at what has been learned by both communities—scholars and practitioners—as their efforts co-evolved. It appears that despite a wealth of possible insights into making cooperation effective very few of the lessons offered by scholars had much impact during the first two decades of climate change diplomacy. Indeed, basic concepts from cooperation theory and evidence from case studies—many developed in Arild’s orbit—can explain why those two decades achieved very little real cooperation. The new Paris agreement may be changing all that and much better reflects insights from scholars about how to build effective international institutions. Success in the Paris process is far from assured and scholars can contribute a lot more with a more strategic view of when and how they have an impact.

Integrated assessment of farm level adaptation to climate change in agriculture

NARCIS (Netherlands)

Mandryk, M.

2016-01-01

The findings of the thesis allowed assessing plausible futures of agriculture in Flevoland around 2050 with insights in effective adaptation to climate change at different levels. Besides empirical findings, this thesis contributed methodologically to the portfolio of climate change impact and

The new socio-economic scenarios for climate change research

International Nuclear Information System (INIS)

Guivarch, C.; Rozenberg, J.

2013-01-01

The scientific community is developing a new generation of scenarios to inform the choices we have to make when it comes to responding to climate change. This new generation of scenarios integrates more fully the mechanisms that regulate climate and provides insights to spatial and temporal resolutions unexplored in previous exercises. In addition, it gives a framework for integrating explicit climate policies for mitigation and adaptation, which allows assessing the benefits and costs of climate policies in different socio-economic scenarios. Finally, it introduces a new way of working that strengthens the collaboration between different research communities on climate change. (authors)

The Data Platform for Climate Research and Action: Introducing Climate Watch

Science.gov (United States)

Hennig, R. J.; Ge, M.; Friedrich, J.; Lebling, K.; Carlock, G.; Arcipowska, A.; Mangan, E.; Biru, H.; Tankou, A.; Chaudhury, M.

2017-12-01

The Paris Agreement, adopted through Decision 1/CP.21, brings all nations together to take on ambitious efforts to combat climate change. Open access to climate data supporting climate research, advancing knowledge, and informing decision making is key to encourage and strengthen efforts of stakeholders at all levels to address and respond to effects of climate change. Climate Watch is a robust online data platform developed in response to the urgent needs of knowledge and tools to empower climate research and action, including those of researchers, policy makers, the private sector, civil society, and all other non-state actors. Building on the rapid growing technology of open data and information sharing, Climate Watch is equipped with extensive amount of climate data, informative visualizations, concise yet efficient user interface, and connection to resources users need to gather insightful information on national and global progress towards delivering on the objective of the Convention and the Paris Agreement. Climate Watch brings together hundreds of quantitative and qualitative indicators for easy explore, visualize, compare, download at global, national, and sectoral levels: Greenhouse gas (GHG) emissions for more than 190 countries over the1850-2014 time period, covering all seven Kyoto Gases following IPCC source/sink categories; Structured information on over 150 NDCs facilitating the clarity, understanding and transparency of countries' contributions to address climate change; Over 6500 identified linkages between climate actions in NDCs across the 169 targets of the sustainable development goals (SDG); Over 200 indicators describing low carbon pathways from models and scenarios by integrated assessment models (IAMs) and national sources; and Data on vulnerability and risk, policies, finance, and many more. Climate Watch platform is developed as part of the broader efforts within the World Resources Institute, the NDC Partnership, and in collaboration

Understanding and managing trust at the climate science-policy interface

Science.gov (United States)

Lacey, Justine; Howden, Mark; Cvitanovic, Christopher; Colvin, R. M.

2018-01-01

Climate change effects are accelerating, making the need for appropriate actions informed by sound climate knowledge ever more pressing. A strong climate science-policy relationship facilitates the effective integration of climate knowledge into local, national and global policy processes, increases society's responsiveness to a changing climate, and aligns research activity to policy needs. This complex science-policy relationship requires trust between climate science `producers' and `users', but our understanding of trust at this interface remains largely uncritical. To assist climate scientists and policymakers, this Perspective provides insights into how trust develops and operates at the interface of climate science and policy, and examines the extent to which trust can manage — or even create — risk at this interface.

Economics, ethics, and climate policy

Energy Technology Data Exchange (ETDEWEB)

Howarth, R.B.; Monahan, P.A.

1992-11-01

Are the costs of greenhouse gas emissions abatement justified by the perceived benefits of sustained climate stability Do people of the present generation have a moral right to impose climate risks on their descendants in generations to come This report examines these questions in light of the emergent facts of climate science and their socioeconomic implications. We consider alternative normative criteria for social decision-making with particular emphasis on cost-benefit analysis and the principle of sustainable development. While each framework yields important insights, we argue that the gross uncertainties associated with climate change and the distribution of impacts between present and future generations constrain the usefulness of cost-benefit criteria in evaluating climate policy. If one accepts the ethical proposition that it is morally wrong to impose catastrophic risks on unborn generations when reducing those risks would not noticeably diminish the quality of life of existing persons, a case can be made for concerted policy action to reduce greenhouse gas emissions.

Economics, ethics, and climate policy

Energy Technology Data Exchange (ETDEWEB)

Howarth, R.B.; Monahan, P.A.

1992-11-01

Are the costs of greenhouse gas emissions abatement justified by the perceived benefits of sustained climate stability? Do people of the present generation have a moral right to impose climate risks on their descendants in generations to come? This report examines these questions in light of the emergent facts of climate science and their socioeconomic implications. We consider alternative normative criteria for social decision-making with particular emphasis on cost-benefit analysis and the principle of sustainable development. While each framework yields important insights, we argue that the gross uncertainties associated with climate change and the distribution of impacts between present and future generations constrain the usefulness of cost-benefit criteria in evaluating climate policy. If one accepts the ethical proposition that it is morally wrong to impose catastrophic risks on unborn generations when reducing those risks would not noticeably diminish the quality of life of existing persons, a case can be made for concerted policy action to reduce greenhouse gas emissions.

Country, climate change adaptation and colonisation: insights from an Indigenous adaptation planning process, Australia.

Science.gov (United States)

Nursey-Bray, Melissa; Palmer, Robert

2018-03-01

Indigenous peoples are going to be disproportionately affected by climate change. Developing tailored, place based, and culturally appropriate solutions will be necessary. Yet finding cultural and institutional 'fit' within and between competing values-based climate and environmental management governance regimes remains an ongoing challenge. This paper reports on a collaborative research project with the Arabana people of central Australia, that resulted in the production of the first Indigenous community-based climate change adaptation strategy in Australia. We aimed to try and understand what conditions are needed to support Indigenous driven adaptation initiatives, if there are any cultural differences that need accounting for and how, once developed they be integrated into existing governance arrangements. Our analysis found that climate change adaptation is based on the centrality of the connection to 'country' (traditional land), it needs to be aligned with cultural values, and focus on the building of adaptive capacity. We find that the development of climate change adaptation initiatives cannot be divorced from the historical context of how the Arabana experienced and collectively remember colonisation. We argue that in developing culturally responsive climate governance for and with Indigenous peoples, that that the history of colonisation and the ongoing dominance of entrenched Western governance regimes needs acknowledging and redressing into contemporary environmental/climate management.

Phenology and growth of European trees in relation to climate change

NARCIS (Netherlands)

Kramer, K.

1996-01-01

Research topics

The relationships between climate and both phenology and growth of some important European tree species were studied to evaluate the potential impacts of climate change on trees and forests in Europe. In order to make such assessments, insight is

Climate Change and Regulation in International and Regional Level, Especially the Built Environment

Directory of Open Access Journals (Sweden)

Putnoki Zsuzsanna

2015-12-01

Full Text Available The article starts with a brief insight into the history of climate change, with a scope on the international and legal aspects of ever-changing regulations. The regional level is in the article is The European Union, as the only regional economic integration organization under the Kyoto Protocol. It deals with the United Nation’s international agreements like UNFCCC its Kyoto’s Protocol and the Post-Kyoto era. It also analyses the EU’s system in the climate change law with correspondence the international rules. Comparison between international and regional legislation in the climate change is used as a tool of analysis. Finally an insight is given into a special field in the climate change, the build environment, reflecting on the related United Nation’s recommendation and the EU’s regulation.

Strengthening Climate Services Capabilities and Regional Engagement at NOAA's National Climatic Data Center

Science.gov (United States)

Shea, E.

2008-12-01

The demand for sector-based climate information is rapidly expanding. In order to support this demand, it is crucial that climate information is managed in an effective, efficient, and user-conscious manner. NOAA's National Climatic Data Center is working closely with numerous partners to develop a comprehensive interface that is authoritative, accessible, and responsive to a variety of sectors, stakeholders, and other users. This talk will explore these dynamics and activities, with additional perspectives on climate services derived from the regional and global experiences of the NOAA Integrated Data and Environmental Applications (IDEA) Center in the Pacific. The author will explore the importance of engaging partners and customers in the development, implementation and emergence of a national climate service program. The presentation will draw on the author's experience in climate science and risk management programs in the Pacific, development of regional and national climate services programs and insights emerging from climate services development efforts in NCDC. In this context, the author will briefly discuss some of guiding principles for effective climate services and applications including: - Early and continuous dialogue, partnership and collaboration with users/customers; - Establishing and sustaining trust and credibility through a program of shared learning and joint problem- solving; - Understanding the societal context for climate risk management and using a problem-focused approach to the development of products and services; - Addressing information needs along a continuum of timescales from extreme events to long-term change; and - Embedding education, outreach and communications activities as critical program elements in effective climate services. By way of examples, the author will reference lessons learned from: early Pacific Island climate forecast applications and climate assessment activities; the implementation of the Pacific Climate

Facing future climate change: is the past relevant?

Science.gov (United States)

Skinner, Luke

2008-12-28

From a socio-economic perspective, the 'sharp end' of climate research is very much about looking forward in time. As far as possible, we need to know what to expect and approximately when to expect it. However, it is argued here that our approach to climate change (including its scientific basis and its policy implications) is firmly linked to our understanding of the past. This is mainly due to the role played by palaeoclimate reconstructions in shaping our expectations of the climate system, in particular via their ability to test the accuracy of our climate models. Importantly, this includes the intuitive models that each of us carries around in our mind, as well as the more complex numerical models hiding inside supercomputers. It is through such models that palaeoclimate insights may affect the scientific and political judgements that we must make in the face of persistent and ultimately irreducible predictive uncertainty. Already we can demonstrate a great deal of confidence in our current understanding of the global climate system based specifically on insights from the geological record. If further advances are to be made effectively, climate models should take advantage of both past and present constraints on their behaviour, and should be given added credence to the extent that they are compatible with an increasingly rich tapestry of past climatic phenomena. Furthermore, palaeoclimate data should be accompanied by clearly defined uncertainties, and organized in arrays that are capable of speaking directly to numerical models, and their limitations in particular.

Role of ELA region in auto-activation of mutant KIT receptor: a molecular dynamics simulation insight.

Science.gov (United States)

Purohit, Rituraj

2014-01-01

KIT receptor is the prime target in gastrointestinal stromal tumor (GISTs) therapy. Second generation inhibitor, Sunitinib, binds to an inactivated conformation of KIT receptor and stabilizes it in order to prevent tumor formation. Here, we investigated the dynamic behavior of wild type and mutant D816H KIT receptor, and emphasized the extended A-loop (EAL) region (805-850) by conducting molecular dynamics simulation (∼100 ns). We analyzed different properties such as root mean square cutoff or deviation, root mean square fluctuation, radius of gyration, solvent-accessible surface area, hydrogen bonding network analysis, and essential dynamics. Apart from this, clustering and cross-correlation matrix approach was used to explore the conformational space of the wild type and mutant EAL region of KIT receptor. Molecular dynamics analysis indicated that mutation (D816H) was able to alter intramolecular hydrogen bonding pattern and affected the structural flexibility of EAL region. Moreover, flexible secondary elements, specially, coil and turns were dominated in EAL region of mutant KIT receptor during simulation. This phenomenon increased the movement of EAL region which in turn helped in shifting the equilibrium towards the active kinase conformation. Our atomic investigation of mutant KIT receptor which emphasized on EAL region provided a better insight into the understanding of Sunitinib resistance mechanism of KIT receptor and would help to discover new therapeutics for KIT-based resistant tumor cells in GIST therapy.

New insights into the use of stable water isotopes at the northern Antarctic Peninsula as a tool for regional climate studies

Science.gov (United States)

Fernandoy, Francisco; Tetzner, Dieter; Meyer, Hanno; Gacitúa, Guisella; Hoffmann, Kirstin; Falk, Ulrike; Lambert, Fabrice; MacDonell, Shelley

2018-03-01

Due to recent atmospheric and oceanic warming, the Antarctic Peninsula is one of the most challenging regions of Antarctica to understand in terms of both local- and regional-scale climate signals. Steep topography and a lack of long-term and in situ meteorological observations complicate the extrapolation of existing climate models to the sub-regional scale. Therefore, new techniques must be developed to better understand processes operating in the region. Isotope signals are traditionally related mainly to atmospheric conditions, but a detailed analysis of individual components can give new insight into oceanic and atmospheric processes. This paper aims to use new isotopic records collected from snow and firn cores in conjunction with existing meteorological and oceanic datasets to determine changes at the climatic scale in the northern extent of the Antarctic Peninsula. In particular, a discernible effect of sea ice cover on local temperatures and the expression of climatic modes, especially the Southern Annular Mode (SAM), is demonstrated. In years with a large sea ice extension in winter (negative SAM anomaly), an inversion layer in the lower troposphere develops at the coastal zone. Therefore, an isotope-temperature relationship (δ-T) valid for all periods cannot be obtained, and instead the δ-T depends on the seasonal variability of oceanic conditions. Comparatively, transitional seasons (autumn and spring) have a consistent isotope-temperature gradient of +0.69 ‰ °C-1. As shown by firn core analysis, the near-surface temperature in the northern-most portion of the Antarctic Peninsula shows a decreasing trend (-0.33 °C year-1) between 2008 and 2014. In addition, the deuterium excess (dexcess) is demonstrated to be a reliable indicator of seasonal oceanic conditions, and therefore suitable to improve a firn age model based on seasonal dexcess variability. The annual accumulation rate in this region is highly variable, ranging between 1060 and 2470 kg m

Molecular characteristics of Streptococcus agalactiae in a mother-baby prospective cohort study: Implication for vaccine development and insights into vertical transmission.

Science.gov (United States)

Li, Shunming; Wen, Guoming; Cao, Xuelian; Guo, Dan; Yao, Zhenjiang; Wu, Chuan'an; Ye, Xiaohua

2018-04-05

Streptococcus agalactiae (GBS) is a leading cause of neonatal sepsis and meningitis in many countries. This study aimed to determine the molecular characteristics of GBS colonized in mothers and their infants so as to provide implication for vaccine strategies and confirm vertical transmission. A prospective cohort study was conducted to recruit 1815 mother-neonate pairs. All GBS isolates from pregnant women and her infants were tested for serotypes, multilocus sequence types and virulence genes. The relationship between multiple molecular characteristics of GBS isolates was tested by the correspondence analysis, and the agreement between mother-neonate paired data in molecular characteristics was analyzed using Kappa tests. The predominant serotypes were III, Ia and V, and the most prevalent sequence types (STs) were ST19, ST17, ST10, and ST12. All isolates carried at least one pilus island (PI). The most common combination of PIs was PI-2b alone, followed by PI-1+PI-2a and PI-2a alone, and the most prevalent alpha-like protein (alp) genes were rib, epsilon and alphaC. Moreover, a strong relationship was noted between STs, serotypes, alp genes and PIs, including ST17 associated with serotype-III/rib/PI-2b, ST19 with serotype-III/rib/PI-1+PI-2a, and ST485 with serotype-Ia/epsilon/PI-2b. The rate of GBS vertical transmission was 14.1%, and the kappa test revealed good agreement in multiple molecular characteristics among GBS-positive mother-neonate pairs. Notably, the switching of molecular characteristics was found during vertical transmission. Our findings underscore the value of monitoring multiple molecular characteristics so as to provide implication for multivalent strategies and gain insights into GBS vertical transmission and vertical characteristic switching. Copyright © 2018 Elsevier Ltd. All rights reserved.

Postglacial Human resilience and susceptibility to abrupt climate change new insights from Star Carr

Science.gov (United States)

Blockley, Simon; Abrook, Ashley; Bayliss, Alex; Candy, Ian; Conneller, Chantal; Darvill, Chris; Deeprose, Laura; Kearney, Rebecca; Langdon, Pete; Langdon Langdon, Cath; Lincoln, Paul; Macleod, Alison; Matthews, Ian; Palmer, Adrian; Schreve, Danielle; Taylor, Barry; Milner, Nicky

2017-04-01

We know little about the lives of the early humans who lived during the early Postglacial period (the Lateglacial and Early Holocene), a time characterised by abrupt climate change after 16,000, which includes a series of abrupt climatic transitions linked to the reorganisation of the global environment after the glacial maximum and the last major global warming event at the onset of the Holocene. The hunter-gatherers who lived during the early Postglacial have been characterised as highly mobile, dispersed and living within small groups, and there is much debate as to how they adapted to climatic and environmental change: did they move in response to climatic transitions (and if so what was the climatic threshold), or instead adapt their lifeways to the new environmental conditions? A key area for examining these ideas is the British Isles as it sits on the Atlantic fringe of Northwest Europe with a climate that is highly responsive to the wider climate forcing experienced in the northern Hemisphere. Furthermore, in this period, Britain is directly linked to continental Europe due to lowered global sea levels allowing for the ease of human migration in and out of this region. In general the British record has been seen as being dominated by abandonment and reoccupation in the Postglacial during periods of climatic transition with hunter-gatherer mobility being closely linked to the prevailing environment. Recent discoveries at the Early Mesolithic site of Star Carr and surrounding area, linked to local and regional climate records, based on isotopic, chironomid and pollen proxy data and dated at high chronological resolution, offer a new picture. Postglacial human occupation of the area commences at the Pleistocene/Holocene transition but is short lived and appears to end close to the Pre-Boreal Oscillation, However, this is followed by a period where hunter-gatherers occupy Star Carr and settle and invest time and effort into building huts and large scale wooden

Molecular mechanics calculations on cobalt phthalocyanine dimers

NARCIS (Netherlands)

Heuts, J.P.A.; Schipper, E.T.W.M.; Piet, P.; German, A.L.

1995-01-01

In order to obtain insight into the structure of cobalt phthalocyanine dimers, molecular mechanics calculations were performed on dimeric cobalt phthalocyanine species. Molecular mechanics calculations are first presented on monomeric cobalt(II) phthalocyanine. Using the Tripos force field for the

Doliroside A from Dolichos falcata Klein suppressing amyloid β-protein 42 fibrillogenesis: An insight at molecular level.

Directory of Open Access Journals (Sweden)

Dongpu Li

Full Text Available A bioactive chemical constituent, doliroside A, from Chinese traditional herbal medicine Dolichos falcata Klein was isolated, purified and identified by 60% ethanol extraction, thin layer chromatography (TLC, high performance liquid chromatography (HPLC and nuclear magnetic resonance (NMR spectroscopy. Molecular interaction mechanism between doliroside and amyloid β42 protein was evaluated by thioflavin T fluorescence (ThT, circular dichroism (CD, atomic force microscope (AFM, and differential scanning calorimeter (DSC from the aspects of kinetics, secondary structure, morphology, and thermodynamics, respectively. Results show that the purity of doliroside A is 99.9% by HPLC, and its chemical structure is identified by 1H- and 13C-NMR. Doliroside A is observed to be concentration-dependent inhibiting the fibrillation of Aβ42 with the IC50 value of 26.57 ± 1.6 μM. CD and DSC results imply that doliroside A can bind to the nuclei and oligomers of Aβ42 to form a stable complex and suppress Aβ42 fibrillation. AFM images show that doliroside A, after bound to the nuclei and oligomers, redirect Aβ42 into off-pathway, amorphous oligomers. These findings not only provide a full insight into the molecular interaction mechanisms between Aβ42 and doliroside A, but also facilitate the development of new native anti-AD drug of doliroside A compound.

Insights into structural and dynamical features of water at halloysite interfaces probed by DFT and classical molecular dynamics simulations.

Science.gov (United States)

Presti, Davide; Pedone, Alfonso; Mancini, Giordano; Duce, Celia; Tiné, Maria Rosaria; Barone, Vincenzo

2016-01-21

Density functional theory calculations and classical molecular dynamics simulations have been used to investigate the structure and dynamics of water molecules on kaolinite surfaces and confined in the interlayer of a halloysite model of nanometric dimension. The first technique allowed us to accurately describe the structure of the tetrahedral-octahedral slab of kaolinite in vacuum and in interaction with water molecules and to assess the performance of two widely employed empirical force fields to model water/clay interfaces. Classical molecular dynamics simulations were used to study the hydrogen bond network structure and dynamics of water adsorbed on kaolinite surfaces and confined in the halloysite interlayer. The results are in nice agreement with the few experimental data available in the literature, showing a pronounced ordering and reduced mobility of water molecules at the hydrophilic octahedral surfaces of kaolinite and confined in the halloysite interlayer, with respect to water interacting with the hydrophobic tetrahedral surfaces and in the bulk. Finally, this investigation provides new atomistic insights into the structural and dynamical properties of water-clay interfaces, which are of fundamental importance for both natural processes and industrial applications.

Modeling Uncertainty in Climate Change: A Multi-Model Comparison

Energy Technology Data Exchange (ETDEWEB)

Gillingham, Kenneth; Nordhaus, William; Anthoff, David; Blanford, Geoffrey J.; Bosetti, Valentina; Christensen, Peter; McJeon, Haewon C.; Reilly, J. M.; Sztorc, Paul

2015-10-01

The economics of climate change involves a vast array of uncertainties, complicating both the analysis and development of climate policy. This study presents the results of the first comprehensive study of uncertainty in climate change using multiple integrated assessment models. The study looks at model and parametric uncertainties for population, total factor productivity, and climate sensitivity and estimates the pdfs of key output variables, including CO₂ concentrations, temperature, damages, and the social cost of carbon (SCC). One key finding is that parametric uncertainty is more important than uncertainty in model structure. Our resulting pdfs also provide insight on tail events.

Improvement with insight. Healthier indoor climate and lower energy consumption with wireless Sensor networks; Verbeteren met inzicht. Gezonder binnenklimaat en lager energiegebruik met draadloze Sensor netwerken

Energy Technology Data Exchange (ETDEWEB)

Peekstok, E.J. [MakeMoreSense, Sommelsdijk (Netherlands)

2010-11-15

Wireless Sensor Networks connected to the internet will dramatically influence the future of Indoor Climate and Energy use. Munisense, a Dutch company, has developed a real-time and web-based monitoring tool called InSightNow. With Insight it is possible to improve the environment, often with very simple measures. The majority of things going wrong are caused by human interventions. For example engineers when programming installations and users adjusting settings. Giving people insight in their behaviour via a 'dashboard for buildings', creates awareness and makes it possible to improve. Installers are able to limit failure cost, improve quality of service and contribute to the customers success by limiting sick-leave, improve work performance, reduce energy cost and unused floor space. [Dutch] Het is noodzakelijk om in schoolgebouwen een blijvend gezond binnenklimaat te creeren, want binnenklimaat, ziekteverzuim en leerprestaties hebben volgens wetenschappelijk onderzoek een direct verband met elkaar. Netwerken met draadloze sensors kunnen daar een rol bij spelen.

A Regional Observatory for Producers' Climate Change Adaptation ...

International Development Research Centre (IDRC) Digital Library (Canada)

2016-04-22

Apr 22, 2016 ... A Regional Observatory for Producers' Climate Change Adaptation in Thies, Senegal ... The Adaptation Insights series is a joint publication of the International Development Research Centre and the Centre for ... Innovation.

Molecular mechanisms involved in convergent crop domestication.

Science.gov (United States)

Lenser, Teresa; Theißen, Günter

2013-12-01

Domestication has helped to understand evolution. We argue that, vice versa, novel insights into evolutionary principles could provide deeper insights into domestication. Molecular analyses have demonstrated that convergent phenotypic evolution is often based on molecular changes in orthologous genes or pathways. Recent studies have revealed that during plant domestication the causal mutations for convergent changes in key traits are likely to be located in particular genes. These insights may contribute to defining candidate genes for genetic improvement during the domestication of new plant species. Such efforts may help to increase the range of arable crops available, thus increasing crop biodiversity and food security to help meet the predicted demands of the continually growing global population under rapidly changing environmental conditions. Copyright © 2013 Elsevier Ltd. All rights reserved.

Addressing the trade-climate change-energy nexus: China's explorations in a global governance landscape

Directory of Open Access Journals (Sweden)

Joachim Monkelbaan

2014-12-01

As a way forward, different approaches towards the governance of trade and climate change will be highlighted. Besides discussing the specific aspects of Chinese participation in global trade and climate change governance, this paper aims at offering broader insights into the nexus between trade, energy and climate governance in China.

Climate Change: From Science to Practice.

Science.gov (United States)

Wheeler, Nicola; Watts, Nick

2018-03-01

Climate change poses a significant threat to human health. Understanding how climate science can be translated into public health practice is an essential first step in enabling robust adaptation and improving resiliency to climate change. Recent research highlights the importance of iterative approaches to public health adaptation to climate change, enabling uncertainties of health impacts and barriers to adaptation to be accounted for. There are still significant barriers to adaptation, which are context-specific and thus present unique challenges to public health practice. The implementation of flexible adaptation approaches, using frameworks targeted for public health, is key to ensuring robust adaptation to climate change in public health practice. The BRACE framework provides an excellent approach for health adaptation to climate change. Combining this with the insights provided and by the adaptation pathways approach allows for more deliberate accounting of long-term uncertainties. The mainstreaming of climate change adaptation into public health practice and planning is important in facilitating this approach and overcoming the significant barriers to effective adaptation. Yet, the immediate and future limits to adaptation provide clear justification for urgent and accelerated efforts to mitigate climate change.

Climate change: Recent findings

International Nuclear Information System (INIS)

Hesselmans, G.H.F.M.

1993-08-01

In the late eighties several reports have been published on climate change and sea level rise. In the meantime insights may have changed due to the availability of better and more observations and/or more advanced climate models. The aim of this report is to present the most recent findings with respect to climate change, in particular of sea level rise, storm surges and river peak flows. These climate factors are important for the safety of low-lying areas with respect to coastal erosion and flooding. In the first chapters a short review is presented of a few of the eighties reports. Furthermore, the predictions by state of the art climate models at that time are given. The reports from the eighties should be considered as 'old' information, whereas the IPCC supplement and work, for example, by Wigley should be considered as new information. To assess the latest findings two experts in this field were interviewed: dr J. Oerlemans and dr C.J.E. Schuurmans, a climate expert from the Royal Netherlands Meteorological Institute (KNMI). Their views are presented together with results published in recent papers on the subject. On the basis of this assessment, the report presents current knowledge regarding predictions of climate change (including sea-level rise) over the next century, together with an assessment of the uncertainties associated with these predictions. 14 figs., 11 tabs., 24 refs

Coastal Risk Management in a Changing Climate

DEFF Research Database (Denmark)

Existing coastal management and defense approaches are not well suited to meet the challenges of climate change and related uncertanities. Professionals in this field need a more dynamic, systematic and multidisciplinary approach. Written by an international group of experts, "Coastal Risk...... Management in a Changing Climate" provides innovative, multidisciplinary best practices for mitigating the effects of climate change on coastal structures. Based on the Theseus program, the book includes eight study sites across Europe, with specific attention to the most vulnerable coastal environments...... such as deltas, estuaries and wetlands, where many large cities and industrial areas are located. Integrated risk assessment tools for considering the effects of climate change and related uncertainties. Presents latest insights on coastal engineering defenses. Provides integrated guidelines for setting up...

Climate smartness in agro-food standards

NARCIS (Netherlands)

Ingram, V.J.; Bezlepkina, I.; Verburg, R.W.

2013-01-01

This report contributes to the BO-10-020-003 project 'Aligning good agricultural practices and climate smart agriculture' commissioned by the Dutch Ministry of Economic Affairs and led by Plant Research International. The aim of this memorandum is to provide insight into the question who determines

Coupled Ethical-Epistemic Analysis of Climate Change

Science.gov (United States)

Vezer, M.

2015-12-01

Are there inherent limitations to what we can know about how the climate will change in the years ahead? How can we use what is known about the future climate in a way that promotes ethical decision-making? These questions call for urgent attention because important policy decisions need to be made in order to prepare for climate change in North America and around the world. While the science of climate change is central to this line of inquiry, the fields of epistemology, moral, political and environmental philosophy may provide insights on how these issues should be addressed. Detailing the relationship between evidential and ethical dimensions of climate change, this research aims to improve our understanding of the interconnections among several lines of inquiry and to develop solutions to problems of decision-making under conditions of scientific uncertainty.

The Political Economy of Health Co-Benefits: Embedding Health in the Climate Change Agenda

Science.gov (United States)

Workman, Annabelle; Blashki, Grant; Bowen, Kathryn J.; Karoly, David J.; Wiseman, John

2018-01-01

A complex, whole-of-economy issue such as climate change demands an interdisciplinary, multi-sectoral response. However, evidence suggests that human health has remained elusive in its influence on the development of ambitious climate change mitigation policies for many national governments, despite a recognition that the combustion of fossil fuels results in pervasive short- and long-term health consequences. We use insights from literature on the political economy of health and climate change, the science–policy interface and power in policy-making, to identify additional barriers to the meaningful incorporation of health co-benefits into climate change mitigation policy development. Specifically, we identify four key interrelated areas where barriers may exist in relation to health co-benefits: discourse, efficiency, vested interests and structural challenges. With these insights in mind, we argue that the current politico-economic paradigm in which climate change is situated and the processes used to develop climate change mitigation policies do not adequately support accounting for health co-benefits. We present approaches for enhancing the role of health co-benefits in the development of climate change mitigation policies to ensure that health is embedded in the broader climate change agenda. PMID:29617317

The Political Economy of Health Co-Benefits: Embedding Health in the Climate Change Agenda.

Science.gov (United States)

Workman, Annabelle; Blashki, Grant; Bowen, Kathryn J; Karoly, David J; Wiseman, John

2018-04-04

A complex, whole-of-economy issue such as climate change demands an interdisciplinary, multi-sectoral response. However, evidence suggests that human health has remained elusive in its influence on the development of ambitious climate change mitigation policies for many national governments, despite a recognition that the combustion of fossil fuels results in pervasive short- and long-term health consequences. We use insights from literature on the political economy of health and climate change, the science–policy interface and power in policy-making, to identify additional barriers to the meaningful incorporation of health co-benefits into climate change mitigation policy development. Specifically, we identify four key interrelated areas where barriers may exist in relation to health co-benefits: discourse, efficiency, vested interests and structural challenges. With these insights in mind, we argue that the current politico-economic paradigm in which climate change is situated and the processes used to develop climate change mitigation policies do not adequately support accounting for health co-benefits. We present approaches for enhancing the role of health co-benefits in the development of climate change mitigation policies to ensure that health is embedded in the broader climate change agenda.

Business, Climate Change and Emissions Trading. Taking Stock and Looking Ahead (Editorial)

Energy Technology Data Exchange (ETDEWEB)

Kolk, A. [Business School, University of Amsterdam, Amsterdam (Netherlands); Hoffmann, V. [Swiss Federal Institute of Technology, Zurich (Switzerland)

2007-12-15

This editorial summarizes the articles presented in a special issue of this journal on the title subject. Taken as a whole, the articles in this special issue clearly show that companies have dramatically transformed their attitudes and reactions towards climate change compared to 1997. At the same time, they seem to struggle finding appropriate responses, with some of them questioning whether the changes under way are sufficient and adequate. The complexities, uncertainties, and fragmentation of current climate policy seem to be major factors that impede more pro-active business responses. While policymakers should work towards unifying and simplifying climate policy in order to improve its impact, managers should expect the need for their companies to adapt to a range of climate-related changes in their business environment, present and future. The theoretical and empirical insights offered in this special issue provide a good insight into the current state of knowledge on business and climate change, and the (potential) implications for research and practice. In view of the topical nature of the issue, there is a wide range of areas that deserve further investigation.

Paris after Trump: An Inconvenient Insight

OpenAIRE

Böhringer, Christoph; Rutherford, Thomas F.

2017-01-01

With his announcement to pull the US out of the Paris Agreement US President Donald Trump has snubbed the international climate policy community. Key remaining parties to the Agreement such as Europe and China might call for carbon tariffs on US imports as a sanctioning instrument to coerce US compliance. Our analysis, however, reveals an inconvenient insight for advocates of carbon tariffs: given the possibility of retaliatory tariffs across all imported goods, carbon tariffs do not constitu...

Economics, ethics and climate policy

International Nuclear Information System (INIS)

Howarth, R.B.; Monahan, P.A.

1993-01-01

Are the costs of greenhouse gas emissions abatement justified by the perceived benefits of sustained climate stability? Do people of the present generation have a moral right to impose climate risks on their descendants in generations to come? This report examines these questions in light of the emergent facts of climate science and their socioeconomic implications. We consider alternative normative criteria for social decision-making with particular emphasis on cost-benefit analysis and the principle of sustainable development. While each framework yields important insights, we argue that the gross uncertainties associated with climate change and the distribution of impacts between present and future generations constrain the usefulness of cost-benefit criteria in evaluating climate policy. If one accepts the ethical proposition that it is morally wrong to impose catastrophic risks on unborn generations when reducing those risks would not noticeably diminish the quality of life of existing persons, a case can be made for concerted policy action to reduce greenhouse gas emissions. (118 refs., 3 figs., 4 tabs.)

Molecular Insight into Human Lysozyme and Its Ability to Form Amyloid Fibrils in High Concentrations of Sodium Dodecyl Sulfate: A View from Molecular Dynamics Simulations.

Directory of Open Access Journals (Sweden)

Majid Jafari

Full Text Available Changes in the tertiary structure of proteins and the resultant fibrillary aggregation could result in fatal heredity diseases, such as lysozyme systemic amyloidosis. Human lysozyme is a globular protein with antimicrobial properties with tendencies to fibrillate and hence is known as a fibril-forming protein. Therefore, its behavior under different ambient conditions is of great importance. In this study, we conducted two 500000 ps molecular dynamics (MD simulations of human lysozyme in sodium dodecyl sulfate (SDS at two ambient temperatures. To achieve comparative results, we also performed two 500000 ps human lysozyme MD simulations in pure water as controls. The aim of this study was to provide further molecular insight into all interactions in the lysozyme-SDS complexes and to provide a perspective on the ability of human lysozyme to form amyloid fibrils in the presence of SDS surfactant molecules. SDS, which is an anionic detergent, contains a hydrophobic tail with 12 carbon atoms and a negatively charged head group. The SDS surfactant is known to be a stabilizer for helical structures above the critical micelle concentration (CMC [1]. During the 500000 ps MD simulations, the helical structures were maintained by the SDS surfactant above its CMC at 300 K, while at 370 K, human lysozyme lost most of its helices and gained β-sheets. Therefore, we suggest that future studies investigate the β-amyloid formation of human lysozyme at SDS concentrations above the CMC and at high temperatures.

Distributed Generation to Support Development-Focused Climate Action

Energy Technology Data Exchange (ETDEWEB)

Cox, Sadie; Gagnon, Pieter; Stout, Sherry; Zinaman, Owen; Watson, Andrea; Hotchkiss, Eliza

2016-09-01

This paper explores the role of distributed generation, with a high renewable energy contribution, in supporting low emission climate-resilient development. The paper presents potential impacts on development (via energy access), greenhouse gas emission mitigation, and climate resilience directly associated with distributed generation, as well as specific actions that may enhance or increase the likelihood of climate and development benefits. This paper also seeks to provide practical and timely insights to support distributed generation policymaking and planning within the context of common climate and development goals as the distributed generation landscape rapidly evolves globally. Country-specific distributed generation policy and program examples, as well as analytical tools that can inform efforts internationally, are also highlighted throughout the paper.

Climate change studies and the human sciences

Science.gov (United States)

Holm, Poul; Winiwarter, Verena

2017-09-01

Policy makers have made repeated calls for integration of human and natural sciences in the field of climate change. Serious multidisciplinary attempts began already in the 1950s. Progress has certainly been made in understanding the role of humans in the planetary system. New perspectives have clarified policy advice, and three insights are singled out in the paper: the critique of historicism, the distinction between benign and wicked problems, and the cultural critique of the 'myths of nature'. Nevertheless, analysis of the IPCC Assessment Reports indicates that integration is skewed towards a particular dimension of human sciences (economics) and major insights from cultural theory and historical analysis have not made it into climate science. A number of relevant disciplines are almost absent in the composition of authorship. Nevertheless, selective assumptions and arguments are made about e.g. historical findings in key documents. In conclusion, we suggest to seek remedies for the lack of historical scholarship in the IPCC reports. More effort at science-policy exchange is needed, and an Integrated Platform to channel humanities and social science expertise for climate change research might be one promising way.

The CCPP-ARM Parameterization Testbed (CAPT): Where Climate Simulation Meets Weather Prediction

Energy Technology Data Exchange (ETDEWEB)

Phillips, T J; Potter, G L; Williamson, D L; Cederwall, R T; Boyle, J S; Fiorino, M; Hnilo, J J; Olson, J G; Xie, S; Yio, J J

2003-11-21

To significantly improve the simulation of climate by general circulation models (GCMs), systematic errors in representations of relevant processes must first be identified, and then reduced. This endeavor demands, in particular, that the GCM parameterizations of unresolved processes should be tested over a wide range of time scales, not just in climate simulations. Thus, a numerical weather prediction (NWP) methodology for evaluating model parameterizations and gaining insights into their behavior may prove useful, provied that suitable adaptations are made for implementation in climate GCMs. This method entails the generation of short-range weather forecasts by realistically initialized climate GCM, and the application of six-hourly NWP analyses and observations of parameterized variables to evaluate these forecasts. The behavior of the parameterizations in such a weather-forecasting framework can provide insights on how these schemes might be improved, and modified parameterizations then can be similarly tested. In order to further this method for evaluating and analyzing parameterizations in climate GCMs, the USDOE is funding a joint venture of its Climate Change Prediction Program (CCPP) and Atmospheric Radiation Measurement (ARM) Program: the CCPP-ARM Parameterization Testbed (CAPT). This article elaborates the scientific rationale for CAPT, discusses technical aspects of its methodology, and presents examples of its implementation in a representative climate GCM. Numerical weather prediction methods show promise for improving parameterizations in climate GCMs.

Human T-cell lymphotropic virus type 1 subtype C molecular variants among indigenous australians: new insights into the molecular epidemiology of HTLV-1 in Australo-Melanesia.

Directory of Open Access Journals (Sweden)

Olivier Cassar

Full Text Available BACKGROUND: HTLV-1 infection is endemic among people of Melanesian descent in Papua New Guinea, the Solomon Islands and Vanuatu. Molecular studies reveal that these Melanesian strains belong to the highly divergent HTLV-1c subtype. In Australia, HTLV-1 is also endemic among the Indigenous people of central Australia; however, the molecular epidemiology of HTLV-1 infection in this population remains poorly documented. FINDINGS: Studying a series of 23 HTLV-1 strains from Indigenous residents of central Australia, we analyzed coding (gag, pol, env, tax and non-coding (LTR genomic proviral regions. Four complete HTLV-1 proviral sequences were also characterized. Phylogenetic analyses implemented with both Neighbor-Joining and Maximum Likelihood methods revealed that all proviral strains belong to the HTLV-1c subtype with a high genetic diversity, which varied with the geographic origin of the infected individuals. Two distinct Australians clades were found, the first including strains derived from most patients whose origins are in the North, and the second comprising a majority of those from the South of central Australia. Time divergence estimation suggests that the speciation of these two Australian clades probably occurred 9,120 years ago (38,000-4,500. CONCLUSIONS: The HTLV-1c subtype is endemic to central Australia where the Indigenous population is infected with diverse subtype c variants. At least two Australian clades exist, which cluster according to the geographic origin of the human hosts. These molecular variants are probably of very ancient origin. Further studies could provide new insights into the evolution and modes of dissemination of these retrovirus variants and the associated ancient migration events through which early human settlement of Australia and Melanesia was achieved.

Insights into the catalytic mechanism of dehydrogenase BphB: A quantum mechanics/molecular mechanics study.

Science.gov (United States)

Zhang, Ruiming; Shi, Xiangli; Sun, Yanhui; Zhang, Qingzhu; Wang, Wenxing

2018-05-17

The present study delineated the dehydrogenation mechanism of cis-2,3-dihydro-2,3-dihydroxybiphenyl (2,3-DDBPH) and cis-2,3-dihydro-2,3-dihydroxy-4,4'-dichlorobiphenyl (2,3-DD-4,4'-DBPH) by Pandoraea pnomenusa strain B-356 cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase (BphB) in atomistic detail. The enzymatic process was investigated by a combined quantum mechanics/molecular mechanics (QM/MM) approach. Five different snapshots were extracted and calculated, which revealed that the Boltzmann-weighted average barriers of 2,3-DDBPH and 2,3-DD-4,4'-DBPH dehydrogenation processes are 10.7 and 11.5 kcal mol -1 , respectively. The established dehydrogenation mechanism provides new insight into the degradation processes of other chlorinated 2,3-DDBPH. In addition to Asn115, Ser142, and Lys149, the importance of Ile 89, Asn143, Pro184, Met 187, Thr189, and Lue 191 during the dehydrogenation process of 2,3-DDBPH and 2,3-DD-4,4'-DBPH were also highlighted to search for promising mutation targets for improving the catalytic efficiency of BphB. Copyright © 2018. Published by Elsevier Ltd.

Risk communication, public engagement, and climate change: a role for emotions.

Science.gov (United States)

Roeser, Sabine

2012-06-01

This article discusses the potential role that emotions might play in enticing a lifestyle that diminishes climate change. Climate change is an important challenge for society. There is a growing consensus that climate change is due to our behavior, but few people are willing to significantly adapt their lifestyle. Empirical studies show that people lack a sense of urgency: they experience climate change as a problem that affects people in distant places and in a far future. Several scholars have claimed that emotions might be a necessary tool in communication about climate change. This article sketches a theoretical framework that supports this hypothesis, drawing on insights from the ethics of risk and the philosophy of emotions. It has been shown by various scholars that emotions are important determinants in risk perception. However, emotions are generally considered to be irrational states and are hence excluded from communication and political decision making about risky technologies and climate change, or they are used instrumentally to create support for a position. However, the literature on the ethics of risk shows that the dominant, technocratic approach to risk misses the normative-ethical dimension that is inherent to decisions about acceptable risk. Emotion research shows that emotions are necessary for practical and moral decision making. These insights can be applied to communication about climate change. Emotions are necessary for understanding the moral impact of the risks of climate change, and they also paradigmatically provide for motivation. Emotions might be the missing link in effective communication about climate change. © 2012 Society for Risk Analysis.

Molecular details of secretory phospholipase A2 from flax (Linum usitatissimum L.) provide insight into its structure and function.

Science.gov (United States)

Gupta, Payal; Dash, Prasanta K

2017-09-11

Secretory phospholipase A 2 (sPLA 2 ) are low molecular weight proteins (12-18 kDa) involved in a suite of plant cellular processes imparting growth and development. With myriad roles in physiological and biochemical processes in plants, detailed analysis of sPLA 2 in flax/linseed is meagre. The present work, first in flax, embodies cloning, expression, purification and molecular characterisation of two distinct sPLA 2 s (I and II) from flax. PLA 2 activity of the cloned sPLA 2 s were biochemically assayed authenticating them as bona fide phospholipase A 2 . Physiochemical properties of both the sPLA 2 s revealed they are thermostable proteins requiring di-valent cations for optimum activity.While, structural analysis of both the proteins revealed deviations in the amino acid sequence at C- & N-terminal regions; hydropathic study revealed LusPLA 2 I as a hydrophobic protein and LusPLA 2 II as a hydrophilic protein. Structural analysis of flax sPLA 2 s revealed that secondary structure of both the proteins are dominated by α-helix followed by random coils. Modular superimposition of LusPLA 2 isoforms with rice sPLA 2 confirmed monomeric structural preservation among plant phospholipase A 2 and provided insight into structure of folded flax sPLA 2 s.

Molecular insights into the evolutionary pathway of Vibrio cholerae O1 atypical El Tor variants.

Science.gov (United States)

Kim, Eun Jin; Lee, Dokyung; Moon, Se Hoon; Lee, Chan Hee; Kim, Sang Jun; Lee, Jae Hyun; Kim, Jae Ouk; Song, Manki; Das, Bhabatosh; Clemens, John D; Pape, Jean William; Nair, G Balakrish; Kim, Dong Wook

2014-09-01

Pandemic V. cholerae strains in the O1 serogroup have 2 biotypes: classical and El Tor. The classical biotype strains of the sixth pandemic, which encode the classical type cholera toxin (CT), have been replaced by El Tor biotype strains of the seventh pandemic. The prototype El Tor strains that produce biotype-specific cholera toxin are being replaced by atypical El Tor variants that harbor classical cholera toxin. Atypical El Tor strains are categorized into 2 groups, Wave 2 and Wave 3 strains, based on genomic variations and the CTX phage that they harbor. Whole-genome analysis of V. cholerae strains in the seventh cholera pandemic has demonstrated gradual changes in the genome of prototype and atypical El Tor strains, indicating that atypical strains arose from the prototype strains by replacing the CTX phages. We examined the molecular mechanisms that effected the emergence of El Tor strains with classical cholera toxin-carrying phage. We isolated an intermediary V. cholerae strain that carried two different CTX phages that encode El Tor and classical cholera toxin, respectively. We show here that the intermediary strain can be converted into various Wave 2 strains and can act as the source of the novel mosaic CTX phages. These results imply that the Wave 2 and Wave 3 strains may have been generated from such intermediary strains in nature. Prototype El Tor strains can become Wave 3 strains by excision of CTX-1 and re-equipping with the new CTX phages. Our data suggest that inter-chromosomal recombination between 2 types of CTX phages is possible when a host bacterial cell is infected by multiple CTX phages. Our study also provides molecular insights into population changes in V. cholerae in the absence of significant changes to the genome but by replacement of the CTX prophage that they harbor.

The structure of biodiversity – insights from molecular phylogeography

Directory of Open Access Journals (Sweden)

Hewitt Godfrey M

2004-10-01

Full Text Available Abstract DNA techniques, analytical methods and palaeoclimatic studies are greatly advancing our knowledge of the global distribution of genetic diversity, and how it evolved. Such phylogeographic studies are reviewed from Arctic, Temperate and Tropical regions, seeking commonalities of cause in the resulting genetic patterns. The genetic diversity is differently patterned within and among regions and biomes, and is related to their histories of climatic changes. This has major implications for conservation science.

Energy, the Environment and Behaviour Change: A survey of insights from behavioural economics

OpenAIRE

Baddeley, M.

2011-01-01

Evidence of climate change is largely undisputed but moderating the impacts not only of climate change but also of resource depletion is a complex, multi-faceted problem. Technical solutions will have a large role to play but engineering behaviour change within households and firms is essential to harnessing the potential for energy efficient consumption, production and investment. To inform debates about behavior change, this paper explores some insights from behavioural economics including ...

Molecular Insights into Variable Electron Transfer in Amphibian Cryptochrome

DEFF Research Database (Denmark)

Sjulstok, Emil; Lüdemann, Gesa; Kubař, Tomáš

2018-01-01

are mutated, radical-pair formation is still observed. In this study, we computationally investigate electron-transfer pathways in the X. laevis cryptochrome DASH by extensively equilibrating a previously established homology model using molecular dynamics simulations and then mutating key amino acids......Cryptochrome proteins are activated by the absorption of blue light, leading to the formation of radical pairs through electron transfer in the active site. Recent experimental studies have shown that once some of the amino acid residues in the active site of Xenopus laevis cryptochrome DASH...... involved in the electron transfer. The electron-transfer pathways are then probed by using tight-binding density-functional theory. We report the alternative electron-transfer pathways resolved at the molecular level and, through comparison of amino acid sequences for cryptochromes from different species...

Probability for Weather and Climate

Science.gov (United States)

Smith, L. A.

2013-12-01

Over the last 60 years, the availability of large-scale electronic computers has stimulated rapid and significant advances both in meteorology and in our understanding of the Earth System as a whole. The speed of these advances was due, in large part, to the sudden ability to explore nonlinear systems of equations. The computer allows the meteorologist to carry a physical argument to its conclusion; the time scales of weather phenomena then allow the refinement of physical theory, numerical approximation or both in light of new observations. Prior to this extension, as Charney noted, the practicing meteorologist could ignore the results of theory with good conscience. Today, neither the practicing meteorologist nor the practicing climatologist can do so, but to what extent, and in what contexts, should they place the insights of theory above quantitative simulation? And in what circumstances can one confidently estimate the probability of events in the world from model-based simulations? Despite solid advances of theory and insight made possible by the computer, the fidelity of our models of climate differs in kind from the fidelity of models of weather. While all prediction is extrapolation in time, weather resembles interpolation in state space, while climate change is fundamentally an extrapolation. The trichotomy of simulation, observation and theory which has proven essential in meteorology will remain incomplete in climate science. Operationally, the roles of probability, indeed the kinds of probability one has access too, are different in operational weather forecasting and climate services. Significant barriers to forming probability forecasts (which can be used rationally as probabilities) are identified. Monte Carlo ensembles can explore sensitivity, diversity, and (sometimes) the likely impact of measurement uncertainty and structural model error. The aims of different ensemble strategies, and fundamental differences in ensemble design to support of

The essential interactions between understanding climate variability and climate change

Science.gov (United States)

Neelin, J. D.

2017-12-01

Global change is sometimes perceived as a field separate from other aspects of atmospheric and oceanic sciences. Despite the long history of communication between the scientific communities studying global change and those studying interannual variability and weather, increasing specialization and conflicting societal demands on the fields can put these interactions at risk. At the same time, current trajectories for greenhouse gas emissions imply substantial adaptation to climate change will be necessary. Instead of simply projecting effects to be avoided, the field is increasingly being asked to provide regional-level information for specific adaptation strategies—with associated requirements for increased precision on projections. For extreme events, challenges include validating models for rare events, especially for events that are unprecedented in the historical record. These factors will be illustrated with examples of information transfer to climate change from work on fundamental climate processes aimed originally at timescales from hours to interannual. Work to understand the effects that control probability distributions of moisture, temperature and precipitation in historical weather can yield new factors to examine for the changes in the extremes of these distributions under climate change. Surprisingly simple process models can give insights into the behavior of vastly more complex climate models. Observation systems and model ensembles aimed at weather and interannual variations prove valuable for global change and vice versa. Work on teleconnections in the climate system, such as the remote impacts of El Niño, is informing analysis of projected regional rainfall change over California. Young scientists need to prepare to work across the full spectrum of climate variability and change, and to communicate their findings, as they and our society head for future that is more interesting than optimal.

The relationship between team climate and interprofessional collaboration: preliminary results of a mixed methods study

OpenAIRE

Bailey, Christopher; Agreli, Heloise F.; Peduzzi, Marina

2016-01-01

Relational and organisational factors are key elements of interprofessional collaboration (IPC) and team climate. Few studies have explored the relationship between IPC and team climate. This article presents a study that 10 aimed to explore IPC in primary healthcare teams and understand how the assessment of team climate may provide insights into IPC. A mixed methods study design was adopted. In Stage 1 of the study, team climate was assessed using the Team Climate Inventory with 159 profess...

Market Strategies for Climate Change

Energy Technology Data Exchange (ETDEWEB)

Kolk, A.; Pinkse, J. [Business School, University of Amsterdam, Amsterdam (Netherlands)

2004-06-01

The issue of climate change has attracted increasing business attention in the past decade. Whereas companies initially aimed primarily at influencing the policy debate, corporate strategies increasingly include economic responses. Existing classifications for climate change strategies however still reflect the political, non-market components. Using empirical information from the largest multinational companies worldwide, this article examines current market responses, focusing on the drivers (threats and opportunities) and the actions being taken by companies to address climate change. It also develops a typology of climate strategies that addresses the market dimensions, covering both the aim (strategic intent) and the degree of cooperation (form of organisation). The aim turns out to be either innovation or compensation, while the organisational arrangements to reach this objective can be oriented at the company level (internal), at companies' own supply chain (vertical) or at cooperation with other companies (competitors or companies in other sectors - horizontal). The typology can assist managers in deciding about the strategic option(s) they want to choose regarding climate change, also based on the insights offered by the paper about the current state of activities of other companies worldwide.

Is a Breakthrough on Climate Change Governance on the Horizon?

DEFF Research Database (Denmark)

Figueroa, Maria Josefina

The recently released Fifth Assessment report of the IPCC has highlighted again with unprecedented scope and insight the urgency of addressing climate change. The international community has pledged to devise the next international agreement on climate change by 2015, while the EU and in particular...... the Scandinavian countries have forged ahead advancing a variety of policies to respond to climate change. Similarly, regions, municipalities, and private actors across the world are also contributing to climate governance. This paper asks whether the world is reaching a tipping point where a breakthrough...... on climate change governance is near?. The answer is approached by contrasting the governance model within which the IPCC operates and the conditions of policy and governance interaction toward the more scientific foundations laid out by IPCC, with the range of multilateral climate governance...

Local Perceptions of Climate Variability and Change in Tropical Forests of Papua, Indonesia

Directory of Open Access Journals (Sweden)

Manuel Boissière

2013-12-01

Full Text Available People everywhere experience changes and events that impact their lives. Knowing how they perceive, react, and adapt to climatic changes and events is helpful in developing strategies to support adaptation to climate change. Mamberamo in Papua, Indonesia, is a sparsely populated watershed of 7.8 million hectares possessing rich tropical forests. Our study compares scientific and traditional ecological knowledge (TEK on climate, and analyzes how local people in Mamberamo perceive and react to climatic variations. We compared meteorological data for the region with local views gathered through focus group discussions and interviews in six villages. We explored the local significance of seasonality, climate variability, and climate change. Mamberamo is subject to strikingly low levels of climatic variation; nonetheless local people highlighted certain problematic climate-related events such as floods and droughts. As our results illustrate, the implications vary markedly among villages. People currently consider climate variation to have little impact on their livelihoods when contrasted with other factors, e.g., logging, mining, infrastructure development, and political decentralization. Nonetheless, increased salinity of water supplies, crop loss due to floods, and reduced hunting success are concerns in specific villages. To gain local engagement, adaptation strategies should initially focus on factors that local people already judge important. Based on our results we demonstrate that TEK, and an assessment of local needs and concerns, provide practical insights for the development and promotion of locally relevant adaptation strategies. These insights offer a foundation for further engagement.

Sharing the cost of river basin adaptation portfolios to climate change: Insights from social justice and cooperative game theory

Science.gov (United States)

Girard, Corentin; Rinaudo, Jean-Daniel; Pulido-Velazquez, Manuel

2016-10-01

The adaptation of water resource systems to the potential impacts of climate change requires mixed portfolios of supply and demand adaptation measures. The issue is not only to select efficient, robust, and flexible adaptation portfolios but also to find equitable strategies of cost allocation among the stakeholders. Our work addresses such cost allocation problems by applying two different theoretical approaches: social justice and cooperative game theory in a real case study. First of all, a cost-effective portfolio of adaptation measures at the basin scale is selected using a least-cost optimization model. Cost allocation solutions are then defined based on economic rationality concepts from cooperative game theory (the Core). Second, interviews are conducted to characterize stakeholders' perceptions of social justice principles associated with the definition of alternatives cost allocation rules. The comparison of the cost allocation scenarios leads to contrasted insights in order to inform the decision-making process at the river basin scale and potentially reap the efficiency gains from cooperation in the design of river basin adaptation portfolios.

Climate change and outdoor recreation participation in the Southern United States

Science.gov (United States)

J.M. Bowker; Ashley E. Askew; Neelam Poudyal; Stanley J. Zarnoch; Lynne Seymour; H. Ken Cordell

2014-01-01

In this chapter we begin to assess the potential effects of climate change on future outdoor recreation in the South, a region spanning 13 states from Virginia to Texas (Chapter 1). Our goal is to provide some useful insights about future natural resource-based recreation-an important nontimber product derived from southern forests-in the face of climate change. We...

Impacts of climate change on hydrological regime and water resources management of the Koshi River Basin, Nepal

Directory of Open Access Journals (Sweden)

Laxmi Prasad Devkota

2015-09-01

New hydrological insights for the region: The study found that climate change does not pose major threat on average water availability. However, temporal flow variations are expected to increase in the future. The magnitude of projected flow for given return periods, however, strongly depends on the climate model run considered. The ECHAM05 results show higher flow changes than those estimated from the HADCM3 outputs. A relation was derived to estimate projected flood flow as a function of return period and flow estimated from historical series. Amidst the uncertainties, these predictions provide reasonable insight for re-consideration of design standards or design values of hydraulic structures under climate change.

Resilient Rotterdam. Climate change as a challenge. Report of a workshop

International Nuclear Information System (INIS)

Wardekker, J.A.; De Jong, A.; Van der Sluijs, J.P.

2008-12-01

The objective of the study of the resilience of Rotterdam (the Netherlands) with regard to climate change is twofold: 1) to obtain insight in the concepts resilience and uncertainty; to gain insight in how a resilience oriented approach deals with uncertainties about the future; and (2) putting the resilience oriented approach into operation in a case: the area outside the dike of Rotterdam, the Netherlands, which is designated for new buildings. The objective of the workshop was manifold: Making a small inventory of problems that could arise in the area outside the dike of Rotterdam caused by climate change; thinking about working out the 'resilience approach' in concrete options for climate change adaptation in this area; making an inventory of knowledge questions that come from the people that are involved in the design of the area; applying, testing and assessing a number of 'frame-based methods' for structural thinking about such issues [nl

Nanopatterning by molecular polygons.

Science.gov (United States)

Jester, Stefan-S; Sigmund, Eva; Höger, Sigurd

2011-07-27

Molecular polygons with three to six sides and binary mixtures thereof form long-range ordered patterns at the TCB/HOPG interface. This includes also the 2D crystallization of pentagons. The results provide an insight into how the symmetry of molecules is translated into periodic structures.

Molecular insights into the biology of Greater Sage-Grouse

Science.gov (United States)

Oyler-McCance, Sara J.; Quinn, Thomas W.

2011-01-01

Recent research on Greater Sage-Grouse (Centrocercus urophasianus) genetics has revealed some important findings. First, multiple paternity in broods is more prevalent than previously thought, and leks do not comprise kin groups. Second, the Greater Sage-Grouse is genetically distinct from the congeneric Gunnison sage-grouse (C. minimus). Third, the Lyon-Mono population in the Mono Basin, spanning the border between Nevada and California, has unique genetic characteristics. Fourth, the previous delineation of western (C. u. phaios) and eastern Greater Sage-Grouse (C. u. urophasianus) is not supported genetically. Fifth, two isolated populations in Washington show indications that genetic diversity has been lost due to population declines and isolation. This chapter examines the use of molecular genetics to understand the biology of Greater Sage-Grouse for the conservation and management of this species and put it into the context of avian ecology based on selected molecular studies.

Recent advances in understanding secondary organic aerosol: Implications for global climate forcing: Advances in Secondary Organic Aerosol

Energy Technology Data Exchange (ETDEWEB)

Shrivastava, Manish [Pacific Northwest National Laboratory, Richland Washington USA; Cappa, Christopher D. [Department of Civil and Environmental Engineering, University of California, Davis California USA; Fan, Jiwen [Pacific Northwest National Laboratory, Richland Washington USA; Goldstein, Allen H. [Department of Environmental Science, Policy and Management and Department of Civil and Environmental Engineering, University of California, Berkeley California USA; Guenther, Alex B. [Department of Earth System Science, University of California, Irvine California USA; Jimenez, Jose L. [Cooperative Institute for Research in Environmental Sciences and Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder Colorado USA; Kuang, Chongai [Brookhaven National Laboratory, Upton New York USA; Laskin, Alexander [Pacific Northwest National Laboratory, Richland Washington USA; Martin, Scot T. [School of Engineering and Applied Sciences and Department of Earth and Planetary Sciences, Harvard University, Cambridge Massachusetts USA; Ng, Nga Lee [School of Chemical and Biomolecular Engineering and School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta Georgia USA; Petaja, Tuukka [Department of Physics, University of Helsinki, Helsinki Finland; Pierce, Jeffrey R. [Department of Atmospheric Science, Colorado State University, Fort Collins Colorado USA; Rasch, Philip J. [Pacific Northwest National Laboratory, Richland Washington USA; Roldin, Pontus [Department of Physics, Lund University, Lund Sweden; Seinfeld, John H. [Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena California USA; Shilling, John [Pacific Northwest National Laboratory, Richland Washington USA; Smith, James N. [Department of Earth System Science, University of California, Irvine California USA; Thornton, Joel A. [Department of Atmospheric Sciences, University of Washington, Seattle Washington USA; Volkamer, Rainer [Cooperative Institute for Research in Environmental Sciences and Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder Colorado USA; Wang, Jian [Brookhaven National Laboratory, Upton New York USA; Worsnop, Douglas R. [Aerodyne Research, Inc., Billerica Massachusetts USA; Zaveri, Rahul A. [Pacific Northwest National Laboratory, Richland Washington USA; Zelenyuk, Alla [Pacific Northwest National Laboratory, Richland Washington USA; Zhang, Qi [Department of Environmental Toxicology, University of California, Davis California USA

2017-06-01

mass and number with knowledge of particle properties such as volatility and viscosity are crucial for improving understanding of non-linear SOA-related processes. For example, useful insights can be attained by combining bottom-up information related to the molecular speciation of gas- and particle-phase precursors with top-down insights on size evolution dynamics of SOA. Continuing efforts are also needed to rank the most influential processes affecting SOA lifecycle, so that these processes can be accurately represented in atmospheric chemistry-climate models.

Climate. The earth and its atmosphere in the changing times. 3. upd. ed.

International Nuclear Information System (INIS)

Buchal, Christoph; Schoenwiese, Christian-Dietrich

2016-01-01

The Climate Change Challenge. Throughout the world, great efforts are being made to better understanding the development of the global climate and to model future trends. What characterizes the weather what the climate? How did the climate history of the Earth? What factors are affecting the climate? In the third, updated edition 2016 of the widespread attractive and scientifically-based four-volume nonfiction series ENERGY, AIR, POWER and MOBILITY which clearly explained basic knowledge of the climate system is expanded to include the latest information about the IPCC and keep up-to-date insight into modern research, especially the REKLIM project of the Helmholtz Association of German research Centres. [de

Interacting mechanism of ID3 HLH domain towards E2A/E12 transcription factor – An Insight through molecular dynamics and docking approach

Directory of Open Access Journals (Sweden)

Nishith Saurav Topno

2016-03-01

Full Text Available Inhibitor of DNA binding protein 3 (ID3 has long been characterized as an oncogene that implicates its functional role through its Helix–Loop–Helix (HLH domain upon protein–protein interaction. An insight into the dimerization brought by this domain helps in identifying the key residues that favor the mechanism behind it. Molecular dynamics (MD simulations were performed for the HLH proteins ID3 and Transcription factor E2-alpha (E2A/E12 and their ensemble complex (ID3-E2A/E12 to gather information about the HLH domain region and its role in the interaction process. Further evaluation of the results by Principal Component Analysis (PCA and Free Energy Landscape (FEL helped in revealing residues of E2A/E12: Lys570, Ala595, Val598, and Ile599 and ID3: Glu53, Gln63, and Gln66 buried in their HLH motifs imparting key roles in dimerization process. Furthermore the T-pad analysis results helped in identifying the key fluctuations and conformational transitions using the intrinsic properties of the residues present in the domain region of the proteins thus specifying their crucial role towards molecular recognition. The study provides an insight into the interacting mechanism of the ID3-E2A/E12 complex and maps the structural transitions arising in the essential conformational space indicating the key structural changes within the helical regions of the motif. It thereby describes how the internal dynamics of the proteins might regulate their intrinsic structural features and its subsequent functionality.

The relationship between team climate and interprofessional collaboration: Preliminary results of a mixed methods study.

Science.gov (United States)

Agreli, Heloise F; Peduzzi, Marina; Bailey, Christopher

2017-03-01

Relational and organisational factors are key elements of interprofessional collaboration (IPC) and team climate. Few studies have explored the relationship between IPC and team climate. This article presents a study that aimed to explore IPC in primary healthcare teams and understand how the assessment of team climate may provide insights into IPC. A mixed methods study design was adopted. In Stage 1 of the study, team climate was assessed using the Team Climate Inventory with 159 professionals in 18 interprofessional teams based in São Paulo, Brazil. In Stage 2, data were collected through in-depth interviews with a sample of team members who participated in the first stage of the study. Results from Stage 1 provided an overview of factors relevant to teamwork, which in turn informed our exploration of the relationship between team climate and IPC. Preliminary findings from Stage 2 indicated that teams with a more positive team climate (in particular, greater participative safety) also reported more effective communication and mutual support. In conclusion, team climate provided insights into IPC, especially regarding aspects of communication and interaction in teams. Further research will provide a better understanding of differences and areas of overlap between team climate and IPC. It will potentially contribute for an innovative theoretical approach to explore interprofessional work in primary care settings.

Stratum corneum molecular mobility in the presence of natural moisturizers.

Science.gov (United States)

Björklund, Sebastian; Andersson, Jenny Marie; Pham, Quoc Dat; Nowacka, Agnieszka; Topgaard, Daniel; Sparr, Emma

2014-07-07

The outermost layer of the skin, the stratum corneum (SC), is a lipid-protein membrane that experiences considerable osmotic stress from a dry and cold climate. The natural moisturizing factor (NMF) comprises small and polar substances, which like osmolytes can protect living systems from osmotic stress. NMF is commonly claimed to increase the water content in the SC and thereby protect the skin from dryness. In this work we challenge this proposed mechanism, and explore the influence of NMF on the lipid and protein components in the SC. We employ natural-abundance (13)C solid-state NMR methods to investigate how the SC molecular components are influenced by urea, glycerol, pyrrolidone carboxylic acid (PCA), and urocanic acid (UCA), all of which are naturally present in the SC as NMF compounds. Experiments are performed with intact SC, isolated corneocytes and model lipids. The combination of NMR experiments provides molecularly resolved qualitative information on the dynamics of different SC lipid and protein components. We obtain completely novel molecular information on the interaction of these NMF compounds with the SC lipids and proteins. We show that urea and glycerol, which are also common ingredients in skin care products, increase the molecular mobility of both SC lipids and proteins at moderate relative humidity where the SC components are considerably more rigid in the absence of these compounds. This effect cannot be attributed to increased SC water content. PCA has no detectable effect on SC molecular mobility under the conditions investigated. It is finally shown that the more apolar compound, UCA, specifically influences the mobility of the SC lipid regions. The present results show that the NMF components act to retain the fluidity of the SC molecular components under dehydrating conditions in such a way that the SC properties remain largely unchanged as compared to more hydrated SC. These findings provide a new molecular insight into how small

Insight of Transmembrane Processes of Self-Assembling Nanotubes Based on a Cyclic Peptide Using Coarse Grained Molecular Dynamics Simulation.

Science.gov (United States)

Fu, Yankai; Yan, Tingxuan; Xu, Xia

2017-09-28

Transmembrane self-assembling cyclic peptide (SCP) nanotubes are promising candidates for delivering specific molecules through cell membranes. The detailed mechanisms behind the transmembrane processes, as well as stabilization factors of transmembrane structures, are difficult to elucidate through experiments. In this study, the effects of peptide sequence and oligomeric state on the transmembrane capabilities of SCP nanotubes and the perturbation of embedded SCP nanotubes acting on the membrane were investigated based on coarse grained molecular dynamics simulation. The simulation results reveal that hydrophilic SCP oligomers result in the elevation of the energy barrier while the oligomerization of hydrophobic SCPs causes the reduction of the energy barrier, further leading to membrane insertion. Once SCP nanotubes are embedded, membrane properties such as density, thickness, ordering state and lateral mobility are adjusted along the radial direction. This study provides insight into the transmembrane strategy of SCP nanotubes and sheds light on designing novel transport systems.

Crop epigenetics and the molecular hardware of genotype x environment interactions

Directory of Open Access Journals (Sweden)

Graham John King

2015-11-01

Full Text Available Crop plants encounter thermal environments which fluctuate on a diurnal and seasonal basis. Future climate resilient cultivars will need to respond to thermal profiles reflecting more variable conditions, and harness plasticity that involves regulation of epigenetic processes and complex genomic regulatory networks. Compartmentalisation within plant cells insulates the genomic central processing unit within the interphase nucleus. This review addresses the properties of the chromatin hardware in which the genome is embedded, focusing on the biophysical and thermodynamic properties of DNA, histones and nucleosomes. It explores the consequences of thermal and ionic variation on the biophysical behaviour of epigenetic marks such as DNA cytosine methylation (5mC, and histone variants such as H2A.Z, and how these contribute to maintenance of chromatin integrity in the nucleus, while enabling specific subsets of genes to be regulated. Information is drawn from theoretical molecular in vitro studies as well as model and crop plants and incorporates recent insights into the role epigenetic processes play in mediating between environmental signals and genomic regulation. A preliminary speculative framework is outlined, based on the evidence of what appears a cohesive set of interactions at molecular, biophysical and electrostatic level between the various components contributing to chromatin conformation and dynamics. It proposes that within plant nuclei, general and localised ionic homeostasis plays an important role in maintaining chromatin conformation, whilst maintaining complex genomic regulation that involve specific patterns of epigenetic marks. More generally, reversible changes in DNA methylation appear to be consistent with the ability of nuclear chromatin to manage variation in external ionic and temperature environment. Whilst tentative, this framework provides scope to develop experimental approaches to understand in greater detail the

Molecular interaction of PCB153 to human serum albumin: Insights from spectroscopic and molecular modeling studies

Energy Technology Data Exchange (ETDEWEB)

Han, Chao; Fang, Senbiao; Cao, Huiming; Lu, Yan; Ma, Yaqiong [School of Pharmacy, Lanzhou University, Lanzhou 730000 (China); Wei, Dongfeng [Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700 (China); Xie, Xiaoyun [College of Earth and Environmental Science, Lanzhou University, Lanzhou 730000 (China); Liu, Xiaohua [School of Pharmacy, Lanzhou University, Lanzhou 730000 (China); Li, Xin [College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003 (China); Fei, Dongqing [School of Pharmacy, Lanzhou University, Lanzhou 730000 (China); Zhao, Chunyan, E-mail: zhaochy07@lzu.edu.cn [School of Pharmacy, Lanzhou University, Lanzhou 730000 (China)

2013-03-15

Highlights: ► We identify the binding mode of PCB153 to human serum albumin (HSA). ► Spectroscopic and molecular modeling results reveal that PCB153 binds at the site II. ► The interaction is mainly governed by hydrophobic and hydrogen bond forces. ► The work helps to probe transporting, distribution and toxicity effect of PCBs. -- Abstract: Polychlorinated biphenyls (PCBs) possessed much potential hazard to environment because of its chemical stability and biological toxicity. Here, we identified the binding mode of a representative compound, PCB153, to human serum albumin (HSA) using fluorescence and molecular dynamics simulation methods. The fluorescence study showed that the intrinsic fluorescence of HSA was quenched by addition of PCB153 through a static quenching mechanism. The thermodynamic analysis proved the binding behavior was mainly governed by hydrophobic force. Furthermore, as evidenced by site marker displacement experiments using two probe compounds, it revealed that PCB153 acted exactly on subdomain IIIA (site II) of HSA. On the other hand, the molecular dynamics studies as well as free energy calculations made another important contribution to understand the conformational changes of HSA and the stability of HSA-PCB153 system. Molecular docking revealed PCB153 can bind in a large hydrophobic activity of subdomain IIIA by the hydrophobic interaction and hydrogen bond interactions between chlorine atoms and residue ASN391. The present work provided reasonable models helping us further understand the transporting, distribution and toxicity effect of PCBs when it spread into human blood serum.

The Molecular Era of Surfactant Biology

OpenAIRE

Whitsett, Jeffrey A.

2014-01-01

Advances in the physiology, biochemistry, molecular and cell biology of the pulmonary surfactant system transformed the clinical care and outcome of preterm infants with respiratory distress syndrome. The molecular era of surfactant biology provided genetic insights into the pathogenesis of pulmonary disorders, previously termed “idiopathic” that affect newborn infants, children and adults. Knowledge related to the structure and function of the surfactant proteins and their roles in alveolar ...

Supply chain management in view of climate change: an overview of possible impacts and the road ahead

Directory of Open Access Journals (Sweden)

Thomas K. Dasaklis

2013-09-01

Full Text Available Purpose: The paper aims to provide a general overview of the impacts of climate change upon supply chains and to analyze the implications of climate change for supply chain management in terms of strategic and operational planning. A roadmap of fruitful research approaches is also presented. Design/methodology/approach: The paper makes use of a general review of the relevant literature and, based on a systematic categorization of the findings, looks for useful insights towards the issues of climate change and supply chain management. A framework is drawn for systematically assessing the impacts of climate change upon supply chains and their management, while making suggestions for future research. Findings and Originality/value: Supply chain networks run physical, operational and reputational risks attributed to climate change. Escalation in regulations, market forces and stakeholders’ pressures are paving the way for the decarbonization of supply chains with obvious implications for supply chain management. Supply chain managers should pay special attention to the impacts of climate change on supply chains and academics should further explore the interrelationships between climate change and supply chain design and operations. Research limitations/implications: Additional qualitative research based on grounded theory is suggested for validating and interconnecting the findings with empirical data. Practical implications: The paper provides several insights towards the issues of supply chain management in view of climate change and may serve as an initial basis for exploring future research directions by academics. Practitioners, especially those drafting value-creating supply chain agendas, may also find these insights useful for improving their managerial practices. Originality/value: By providing an original structured overview of the impacts of climate change upon supply chain design and operations management the paper substantiates the

For Me It Was When I Saw a Simple Chart: Former Climate Contrarians Recount What Changed Their Minds

Science.gov (United States)

Kirk, K. B.

2017-12-01

Efforts to advance climate policy in the US have been hindered by a sector of the public that is reluctant to accept the science of anthropogenic climate change. Climate educators, advocates, and policymakers seek to resolve this roadblock through educational efforts and strategic messaging, while social science research strives to understand the causes of resistance on climate change. A discussion on the social media platform, AskReddit, offered a surprising source of insight when a tantalizing question was posed, "Former climate deniers, what changed your mind?" Responses to the query offered a rare glimpse into the process of how people switched camps, outgrew their parents' values, had transformative experiences, or were worn down by mounting scientific evidence. The posts contained 66 examples of people who were initially uncertain or dismissive of climate change, but came to accept the mainstream science. The commenters provided insightful narratives describing the origins of their skeptical beliefs, the rationales for their changing opinions, and the events that caused them to reverse course. Analysis of the comments revealed the primary reasons that influenced people to change their minds. Those were: science and evidence (cited as a factor in 47% of the comments); stewardship for the Earth and concerns about pollution (29%); unusual weather events (21%); and the untrustworthiness of the messengers who claim that climate change is false (17%). Note that several commenters pointed to more than one factor that contributed to their evolving views. While neither the setting nor the sample size allow a robust scientific analysis, these anecdotal accounts offer useful insights on a vexing problem. Learning about the circumstances that lead people to update their thinking can help us improve efforts to communicate the science and policy around climate change. This work is the topic of an article at Yale Climate Connections, https

Molecular Dynamic Modeling and Simulation for Polymers

National Research Council Canada - National Science Library

Harrell, Anthony

2003-01-01

... the mechanical properties of polymers. In particular, the goal was to develop insights as to how a molecular level structure is connected to the bulk properties of materials assuming homogeneity...

Evolution under changing climates: climatic niche stasis despite rapid evolution in a non-native plant.

Science.gov (United States)

Alexander, Jake M

2013-09-22

A topic of great current interest is the capacity of populations to adapt genetically to rapidly changing climates, for example by evolving the timing of life-history events, but this is challenging to address experimentally. I use a plant invasion as a model system to tackle this question by combining molecular markers, a common garden experiment and climatic niche modelling. This approach reveals that non-native Lactuca serriola originates primarily from Europe, a climatic subset of its native range, with low rates of admixture from Asia. It has rapidly refilled its climatic niche in the new range, associated with the evolution of flowering phenology to produce clines along climate gradients that mirror those across the native range. Consequently, some non-native plants have evolved development times and grow under climates more extreme than those found in Europe, but not among populations from the native range as a whole. This suggests that many plant populations can adapt rapidly to changed climatic conditions that are already within the climatic niche space occupied by the species elsewhere in its range, but that evolution to conditions outside of this range is more difficult. These findings can also help to explain the prevalence of niche conservatism among non-native species.

Contributions to advances in blend pellet products (BPP) research on molecular structure and molecular nutrition interaction by advanced synchrotron and globar molecular (Micro)spectroscopy.

Science.gov (United States)

Guevara-Oquendo, Víctor H; Zhang, Huihua; Yu, Peiqiang

2018-04-13

To date, advanced synchrotron-based and globar-sourced techniques are almost unknown to food and feed scientists. There has been little application of these advanced techniques to study blend pellet products at a molecular level. This article aims to provide recent research on advanced synchrotron and globar vibrational molecular spectroscopy contributions to advances in blend pellet products research on molecular structure and molecular nutrition interaction. How processing induced molecular structure changes in relation to nutrient availability and utilization of the blend pellet products. The study reviews Utilization of co-product components for blend pellet product in North America; Utilization and benefits of inclusion of pulse screenings; Utilization of additives in blend pellet products; Application of pellet processing in blend pellet products; Conventional evaluation techniques and methods for blend pellet products. The study focus on recent applications of cutting-edge vibrational molecular spectroscopy for molecular structure and molecular structure association with nutrient utilization in blend pellet products. The information described in this article gives better insight on how advanced molecular (micro)spectroscopy contributions to advances in blend pellet products research on molecular structure and molecular nutrition interaction.

Biophysical and molecular docking insight into the interaction of cytosine β-D arabinofuranoside with human serum albumin

International Nuclear Information System (INIS)

Alam, Parvez; Chaturvedi, Sumit Kumar; Anwar, Tamanna; Siddiqi, Mohammad Khursheed; Ajmal, Mohd Rehan; Badr, Gamal; Mahmoud, Mohamed H.; Hasan Khan, Rizwan

2015-01-01

Interaction of pharmacologically important anticancer drug cytosine β-D arabinofuranoside with human serum albumin (HSA) at physiological pH 7.4 has been studied by utilizing various spectroscopic and molecular docking strategies. Fluorescence results revealed that cytosine β-D arabinofuranoside interacts with HSA through static quenching mechanism with binding affinity of 2.4×10 3 M −1 . The average binding distance between drug and Trp 214 of HSA was found to be 2.23 nm on the basis of the theory of Förster's energy transfer. Synchronous fluorescence data indicated that interaction of drug with HSA changed the microenvironment around the tryptophan residue. UV–visible spectroscopy and circular dichroism results deciphered the complex formation and conformational alterations in the HSA respectively. Dynamic light scattering was utilized to understand the topology of protein in absence and presence of drug. Thermodynamic parameters obtained from isothermal titration calorimetry (ΔH=−26.01 kJ mol −1 and TΔS=6.5 kJ mol −1 ) suggested the involvement of van der Waal interaction and hydrogen bonding. Molecular docking and displacement study with site specific markers suggested that cytosine β-D arabinofuranoside binds to subdomain IB of HSA which is also known as the hemin binding site. This study will be helpful to understand the binding mechanism of cytosine β-D arabinofuranoside with HSA and associated alterations. - Highlights: • Comprehensive insight into the interaction of CBDA with HSA. • The interaction process is spontaneous and exothermic. • The main governing forces for stabilizing HSA–CBDA complex are van der Waal interaction and hydrogen bonding. • CBDA binds at subdomain IB on HSA

Expansion Under Climate Change: The Genetic Consequences.

Science.gov (United States)

Garnier, Jimmy; Lewis, Mark A

2016-11-01

Range expansion and range shifts are crucial population responses to climate change. Genetic consequences are not well understood but are clearly coupled to ecological dynamics that, in turn, are driven by shifting climate conditions. We model a population with a deterministic reaction-diffusion model coupled to a heterogeneous environment that develops in time due to climate change. We decompose the resulting travelling wave solution into neutral genetic components to analyse the spatio-temporal dynamics of its genetic structure. Our analysis shows that range expansions and range shifts under slow climate change preserve genetic diversity. This is because slow climate change creates range boundaries that promote spatial mixing of genetic components. Mathematically, the mixing leads to so-called pushed travelling wave solutions. This mixing phenomenon is not seen in spatially homogeneous environments, where range expansion reduces genetic diversity through gene surfing arising from pulled travelling wave solutions. However, the preservation of diversity is diminished when climate change occurs too quickly. Using diversity indices, we show that fast expansions and range shifts erode genetic diversity more than slow range expansions and range shifts. Our study provides analytical insight into the dynamics of travelling wave solutions in heterogeneous environments.

Predicting phenology by integrating ecology, evolution and climate science

Science.gov (United States)

Pau, Stephanie; Wolkovich, Elizabeth M.; Cook, Benjamin I.; Davies, T. Jonathan; Kraft, Nathan J.B.; Bolmgren, Kjell; Betancourt, Julio L.; Cleland, Elsa E.

2011-01-01

Forecasting how species and ecosystems will respond to climate change has been a major aim of ecology in recent years. Much of this research has focused on phenology — the timing of life-history events. Phenology has well-demonstrated links to climate, from genetic to landscape scales; yet our ability to explain and predict variation in phenology across species, habitats and time remains poor. Here, we outline how merging approaches from ecology, climate science and evolutionary biology can advance research on phenological responses to climate variability. Using insight into seasonal and interannual climate variability combined with niche theory and community phylogenetics, we develop a predictive approach for species' reponses to changing climate. Our approach predicts that species occupying higher latitudes or the early growing season should be most sensitive to climate and have the most phylogenetically conserved phenologies. We further predict that temperate species will respond to climate change by shifting in time, while tropical species will respond by shifting space, or by evolving. Although we focus here on plant phenology, our approach is broadly applicable to ecological research of plant responses to climate variability.

Increasing the usability of climate science in political decision-making

Directory of Open Access Journals (Sweden)

Emily R. Newsom

2016-09-01

Full Text Available Abstract As climate-science graduate students at the University of Washington, we had the opportunity to engage in a political process focused on implementing legislation to reduce greenhouse gas emissions in Washington State. Our insights gained from this rare, first-hand, experience may be particularly relevant to other climate scientists. We argue that inflexible research goals within the United States climate-science community limit the relevance of the knowledge our community creates. The mismatch between climate-science research and the information needs of policy makers, while widely acknowledged in certain domains, has yet to be fully appreciated within many earth science disciplines. Broadening the climate-science training of graduate students to include education on the uses of climate information outside of academic settings would both inform and motivate new research directions, and engender validation of non-traditional research within disciplinary cultures.

The Political Economy of Health Co-Benefits: Embedding Health in the Climate Change Agenda

OpenAIRE

Annabelle Workman; Grant Blashki; Kathryn J. Bowen; David J. Karoly; John Wiseman

2018-01-01

A complex, whole-of-economy issue such as climate change demands an interdisciplinary, multi-sectoral response. However, evidence suggests that human health has remained elusive in its influence on the development of ambitious climate change mitigation policies for many national governments, despite a recognition that the combustion of fossil fuels results in pervasive short- and long-term health consequences. We use insights from literature on the political economy of health and climate chan...

Centennial-scale vegetation and climate changes in the Middle Atlas, Morocco: new insights from multi-proxy investigations at Lake Sidi Ali

Science.gov (United States)

Fletcher, William; Campbell, Jennifer; Joannin, Sebastien; Mischke, Steffen; Zielhofer, Christoph; de Batist, Marc; Mikdad, Abdes

2016-04-01

The karstic lakes of the Middle Atlas, Morocco, represent a valuable archive of environmental and climatic change for Northwest Africa. Here we present the results of centennial-scale palynological and charcoal analyses as part of a multiproxy palaeolimnological study of sediment cores from Lake Sidi Ali in the Middle Atlas, Morocco (33° 03 N, 05° 00 W; 2,080 m a.s.l.). Supported by absolute dating including 23 more than twenty AMS 14C dates on pollen concentrates, the record covers the entire Holocene and offers insights into vegetation and climate change at a regionally unprecedented centennial-scale. Pollen assemblages are dominated by steppic herbs, evergreen oaks (Quercus), junipers (Cupressaceae) and Atlantic cedar (Cedrus atlantica). A long-term evolution of the montane vegetation is recorded, reflecting progressive changes in the dominant arboreal taxa and leading to the full establishment of the emblematic cedar forests of the area during the mid-Holocene by 6000 cal BP. Orbital-scale changes in seasonality and growing season moisture availability linked to declining summer insolation are implicated, with a transition from (a) warm, dry summers associated with summer drought tolerant taxa especially evergreen Quercus, high algal productivity in the lake, and high background levels of microcharcoal reflecting distant fire activity during the early Holocene, to (b) cool, relatively humid summers with dominance of montane conifers, declining algal productivity in the lake, and episodic local fire activity during the mid- to late Holocene. Superimposed on the long-term environmental changes are recurrent centennial-scale fluctuations in vegetation composition, reflecting competitive dynamics between the major taxa, initially between steppic and arboreal elements, and later between the major tree taxa. Parallels with hydrological proxies including stable O and C isotopes suggest common responses to climatic drivers (fluctuations in moisture sources and

Connecting Organic Aerosol Climate-Relevant Properties to Chemical Mechanisms of Sources and Processing

Energy Technology Data Exchange (ETDEWEB)

Thornton, Joel [Univ. of Washington, Seattle, WA (United States)

2015-01-26

The research conducted on this project aimed to improve our understanding of secondary organic aerosol (SOA) formation in the atmosphere, and how the properties of the SOA impact climate through its size, phase state, and optical properties. The goal of this project was to demonstrate that the use of molecular composition information to mechanistically connect source apportionment and climate properties can improve the physical basis for simulation of SOA formation and properties in climate models. The research involved developing and improving methods to provide online measurements of the molecular composition of SOA under atmospherically relevant conditions and to apply this technology to controlled simulation chamber experiments and field measurements. The science we have completed with the methodology will impact the simulation of aerosol particles in climate models.

An Insight towards Conceptual Understanding: Looking into the Molecular Structures of Compounds

Science.gov (United States)

Uyulgan, Melis Arzu; Akkuzu, Nalan

2016-01-01

The subject of molecular structures is one of the most important and complex subject in chemistry which a majority of the undergraduate students have difficulties to understand its concepts and characteristics correctly. To comprehend the molecular structures and their characteristics the students need to understand related subjects such as Lewis…

Contemplating the Future: Building Student Resilience in Climate Change Education

Science.gov (United States)

Allison, E.

2015-12-01

Climate change research has largely focused on the biophysical, economic, and political aspects of the phenomenon, its projected impacts, and the possibilities for adaptation (Carey et al. 2014; Castree et al. 2014). In the classroom, too, climate change is generally presented as a scientific, technological, political, and economic challenge. However, defining climate change as physical challenge, divorced from its cultural causes and responses, forecloses some pathways of inquiry and limits the possibilities for adaptation (Adger et al. 2013). Recent perspectives by the environmental historian Mark Carey and colleagues (2014) and by the geographer Noel Castree and colleagues (2014) contend that ethnographic, narrative, social scientific, and humanistic insights are necessary additions to the climate change policy process and can contribute to deliberate, resilient responses to climate change. Among the humanistic insights needed are strategies and practices to maintain fortitude and persistence in the midst of dispiriting ecological trends. Students facing the "gloom and doom" of climate change data in environmental studies courses can experience negative states of mind such as denial, despair, burnout, and grief. Emerging research, however, demonstrates how contemplative practice can shift consciousness and promote resilience. Contemplative practices are those that consciously direct calm, focused attention. Such practices can build internal resilience, by promoting a greater sense of calm and well-being, decreasing stress, and sharpening focus and concentration. In addition, contemplative practices improve relationships with other people, through increasing compassion and flexibility in thinking. They also strengthen relationships with the surrounding world by increasing our ability to question, explore, and cope with rapid change and complexity. This presentation provides a context for incorporating contemplative practices, including mindfulness exercises

Effect of Heat Stress on Reproduction in Dairy Cows: Insights into the Cellular and Molecular Responses of the Oocyte.

Science.gov (United States)

Roth, Zvi

2017-02-08

Among the components of the female reproductive tract, the ovarian pool of follicles and their enclosed oocytes are highly sensitive to hyperthermia. Heat-induced alterations in small antral follicles can be expressed later as compromised maturation and developmental capacity of the ovulating oocyte. This review summarizes the most up-to-date information on the effects of heat stress on the oocyte with an emphasis on unclear points and open questions, some of which might involve new research directions, for instance, whether preantral follicles are heat resistant. The review focuses on the follicle-enclosed oocytes, provides new insights into the cellular and molecular responses of the oocyte to elevated temperature, points out the role of the follicle microenvironment, and discusses some mechanisms that might underlie oocyte impairment. Mechanisms include nuclear and cytoplasmic maturation, mitochondrial function, apoptotic pathways, and oxidative stress. Understanding the mechanism by which heat stress compromises fertility might enable development of new strategies to mitigate its effects.

Evolution in Australia's mesic biome under past and future climates: Insights from a phylogenetic study of the Australian Rock Orchids (Dendrobium speciosum complex, Orchidaceae).

Science.gov (United States)

Simpson, Lalita; Clements, Mark A; Crayn, Darren M; Nargar, Katharina

2018-01-01

The Australian mesic biome spans c. 33° of latitude along Australia's east coast and ranges and is dissected by historical and contemporary biogeographical barriers. To investigate the impact of these barriers on evolutionary diversification and to predict the impact of future climate change on the distribution of species and genetic diversity within this biome, we inferred phylogenetic relationships within the Dendrobium speciosum complex (Orchidaceae) across its distribution and undertook environmental niche modelling (ENM) under past, contemporary and projected future climates. Neighbor Joining tree inference, NeighborNet and Structure analyses of Amplified Fragment Length Polymorphism (AFLP) profiles for D. speciosum sampled from across its distribution showed that the complex consists of two highly supported main groups that are geographically separated by the St. Lawrence gap, an area of dry sclerophyll forest and woodland. The presence of several highly admixed individuals identified by the Structure analysis provided evidence of genetic exchange between the two groups across this gap. Whereas previous treatments have recognised between one to eleven species, the molecular results support the taxonomic treatment of the complex as a single species with two subspecies. The ENM analysis supported the hypothesis that lineage divergence within the complex was driven by past climatic changes. The St. Lawrence gap represented a stronger biogeographic barrier for the D. speciosum complex during the cool and dry glacial climatic conditions of the Pleistocene than under today's interglacial conditions. Shallow genetic divergence was found within the two lineages, which mainly corresponded to three other biogeographic barriers: the Black Mountain Corridor, Glass House Mountains and the Hunter Valley. Our ENM analyses provide further support for the hypothesis that biogeographic barriers along Australia's east coast were somewhat permeable to genetic exchange due to

Molecular modeling of human neutral sphingomyelinase provides insight into its molecular interactions.

Science.gov (United States)

Dinesh; Goswami, Angshumala; Suresh, Panneer Selvam; Thirunavukkarasu, Chinnasamy; Weiergräber, Oliver H; Kumar, Muthuvel Suresh

2011-01-01

The neutral sphingomyelinase (N-SMase) is considered a major candidate for mediating the stress-induced production of ceramide, and it plays an important role in cell-cycle arrest, apoptosis, inflammation, and eukaryotic stress responses. Recent studies have identified a small region at the very N-terminus of the 55 kDa tumour necrosis factor receptor (TNF-R55), designated the neutral sphingomyelinase activating domain (NSD) that is responsible for the TNF-induced activation of N-SMase. There is no direct association between TNF-R55 NSD and N-SMase; instead, a protein named factor associated with N-SMase activation (FAN) has been reported to couple the TNF-R55 NSD to N-SMase. Since the three-dimensional fold of N-SMase is still unknown, we have modeled the structure using the protein fold recognition and threading method. Moreover, we propose models for the TNF-R55 NSD as well as the FAN protein in order to study the structural basis of N-SMase activation and regulation. Protein-protein interaction studies suggest that FAN is crucially involved in mediating TNF-induced activation of the N-SMase pathway, which in turn regulates mitogenic and proinflammatory responses. Inhibition of N-SMase may lead to reduction of ceramide levels and hence may provide a novel therapeutic strategy for inflammation and autoimmune diseases. Molecular dynamics (MD) simulations were performed to check the stability of the predicted model and protein-protein complex; indeed, stable RMS deviations were obtained throughout the simulation. Furthermore, in silico docking of low molecular mass ligands into the active site of N-SMase suggests that His135, Glu48, Asp177, and Asn179 residues play crucial roles in this interaction. Based on our results, these ligands are proposed to be potent and selective N-SMase inhibitors, which may ultimately prove useful as lead compounds for drug development.

Molecular and cellular insights into Zika virus-related neuropathies.

Science.gov (United States)

Zhou, Kai; Wang, Long; Yu, Di; Huang, Hesuyuan; Ji, Hong; Mo, Xuming

2017-06-01

Zika virus (ZIKV), a relatively elusive Aedes mosquito-transmitted flavivirus, had been brought into spotlight until recent widespread outbreaks accompanied by unexpectedly severe clinical neuropathies, including fetal microcephaly and Guillain-Barré syndrome (GBS) in the adult. In this review, we focus on the underlying cellular and molecular mechanisms by which vertically transmitted microorganisms reach the fetus and trigger neuropathies.

New insights on molecular mechanisms of renal aging.

Science.gov (United States)

Schmitt, R; Melk, A

2012-11-01

Long-term transplant outcome is importantly influenced by the age of the organ donor. The mechanisms how age carries out its pathophysiological impact on graft survival are still not understood. One major contributing factor for the observed poor performance of old donor kidneys seems in particular the age-related loss in renal regenerative capacity. In this review, we will summarize recent findings about the molecular basis of renal aging with specific focus on the potential role of somatic cellular senescence and mitochondrial aging in renal transplant outcome. © Copyright 2012 The American Society of Transplantation and the American Society of Transplant Surgeons.

Reduction of α,β-Unsaturated Ketones by Old Yellow Enzymes: Mechanistic Insights from Quantum Mechanics/Molecular Mechanics Calculations.

Science.gov (United States)

Lonsdale, Richard; Reetz, Manfred T

2015-11-25

Enoate reductases catalyze the reduction of activated C═C bonds with high enantioselectivity. The oxidative half-reaction, which involves the addition of a hydride and a proton to opposite faces of the C═C bond, has been studied for the first time by hybrid quantum mechanics/molecular mechanics (QM/MM). The reduction of 2-cyclohexen-1-one by YqjM from Bacillus subtilis was selected as the model system. Two-dimensional QM/MM (B3LYP-D/OPLS2005) reaction pathways suggest that the hydride and proton are added as distinct steps, with the former step preceding the latter. Furthermore, we present interesting insights into the reactivity of this enzyme, including the weak binding of the substrate in the active site, the role of the two active site histidine residues for polarization of the substrate C═O bond, structural details of the transition states to hydride and proton transfer, and the role of Tyr196 as proton donor. The information presented here will be useful for the future design of enantioselective YqjM mutants for other substrates.

A climate for collaboration. Analysis of US and EU lessons and opportunities in energy and climate policy

International Nuclear Information System (INIS)

De Vita, A.; McLaren, J.; De Coninck, H.C.; Cochran, J.

2009-11-01

This paper aims to improve mutual understanding between the EU and US with regard to climate change and energy policy, suggesting specific opportunities for transatlantic cooperation in this area. A background on the environmental, legislative, and economic contexts of the EU and US as they relate to climate policy sets the context. This is followed by an overview of how cap and trade, renewable energy, and sustainable transportation policies have taken shape in the EU and the US. Some observations and lessons learnt within each of these areas are highlighted. Building on these insights, recommendations are made regarding the carbon market, possibilities for new technologies to bridge the valley of death, and best practices and standards.

Increasing the value of climate finance in an uncertain environment: Diaspora financial resources as a source of climate finance for Sub-Saharan Africa

Directory of Open Access Journals (Sweden)

Dumisani Chirambo

2017-11-01

Full Text Available Climate change may create and increase world inequality and social conflicts. Moreover, regardless of the various developments in science and technology, there are still uncertainties regarding the precise extent and consequences of climate change. Additionally, financial resources to support climate change mitigation, adaptation and capacity building programmes in Africa may be considered to be far from satisfactory in terms of their size, source and distribution. Global policymakers are therefore keen to discover how climate finance resources can be mobilised from a wide variety of sources, instruments and channels to complement public sources. To address this knowledge gap, this paper undertook an analysis of project reports, policy reviews, policy briefs, and academic literature reviews on the barriers and opportunities for private investments in climate change programmes. The paper highlights that Sub-Saharan Africa has the potential to mobilise over US $100 billion annually from its diaspora population, and these funds can be channelled towards climate change investments and programmes or leveraged with other conventional climate finance modalities. This paper therefore provides insights into the measures that can be undertaken in order to enable Africa’s diaspora to become important financiers of climate change programmes.

Uncovering molecular processes in crystal nucleation and growth by using molecular simulation.

Science.gov (United States)

Anwar, Jamshed; Zahn, Dirk

2011-02-25

Exploring nucleation processes by molecular simulation provides a mechanistic understanding at the atomic level and also enables kinetic and thermodynamic quantities to be estimated. However, whilst the potential for modeling crystal nucleation and growth processes is immense, there are specific technical challenges to modeling. In general, rare events, such as nucleation cannot be simulated using a direct "brute force" molecular dynamics approach. The limited time and length scales that are accessible by conventional molecular dynamics simulations have inspired a number of advances to tackle problems that were considered outside the scope of molecular simulation. While general insights and features could be explored from efficient generic models, new methods paved the way to realistic crystal nucleation scenarios. The association of single ions in solvent environments, the mechanisms of motif formation, ripening reactions, and the self-organization of nanocrystals can now be investigated at the molecular level. The analysis of interactions with growth-controlling additives gives a new understanding of functionalized nanocrystals and the precipitation of composite materials. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Evolutionary insights into scleractinian corals using comparative genomic hybridizations.

KAUST Repository

Aranda, Manuel

2012-09-21

Coral reefs belong to the most ecologically and economically important ecosystems on our planet. Yet, they are under steady decline worldwide due to rising sea surface temperatures, disease, and pollution. Understanding the molecular impact of these stressors on different coral species is imperative in order to predict how coral populations will respond to this continued disturbance. The use of molecular tools such as microarrays has provided deep insight into the molecular stress response of corals. Here, we have performed comparative genomic hybridizations (CGH) with different coral species to an Acropora palmata microarray platform containing 13,546 cDNA clones in order to identify potentially rapidly evolving genes and to determine the suitability of existing microarray platforms for use in gene expression studies (via heterologous hybridization).

CRISPR-Cas adaptation: insights into the mechanism of action.

Science.gov (United States)

Amitai, Gil; Sorek, Rotem

2016-02-01

Since the first demonstration that CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against phages and plasmids, numerous studies have yielded key insights into the molecular mechanisms governing how these systems attack and degrade foreign DNA. However, the molecular mechanisms underlying the adaptation stage, in which new immunological memory is formed, have until recently represented a major unresolved question. In this Progress article, we discuss recent discoveries that have shown both how foreign DNA is identified by the CRISPR-Cas adaptation machinery and the molecular basis for its integration into the chromosome to form an immunological memory. Furthermore, we describe the roles of each of the specific CRISPR-Cas components that are involved in memory formation, and consider current models for their evolutionary origin.

Evolutionary insights into scleractinian corals using comparative genomic hybridizations.

KAUST Repository

Aranda, Manuel; DeSalvo, Michael K; Bayer, Till; Medina, Monica; Voolstra, Christian R.

2012-01-01

Coral reefs belong to the most ecologically and economically important ecosystems on our planet. Yet, they are under steady decline worldwide due to rising sea surface temperatures, disease, and pollution. Understanding the molecular impact of these stressors on different coral species is imperative in order to predict how coral populations will respond to this continued disturbance. The use of molecular tools such as microarrays has provided deep insight into the molecular stress response of corals. Here, we have performed comparative genomic hybridizations (CGH) with different coral species to an Acropora palmata microarray platform containing 13,546 cDNA clones in order to identify potentially rapidly evolving genes and to determine the suitability of existing microarray platforms for use in gene expression studies (via heterologous hybridization).

Molecular Diagnostics of Fusion and Laboratory Plasmas

Science.gov (United States)

Fantz, U.

2005-05-01

The presence of molecules in the cold scrape-off layer of fusion experiments and industrial plasmas requires an understanding of the molecular dynamics in these low temperature plasmas. Suitable diagnostic methods can provide an insight in molecular processes in the plasma volume as well as for plasma surface interactions. A very simple but powerful technique is the molecular emission spectroscopy. Spectra are obtained easily, whereas interpretation might be very complex and relies on the availability of atomic and molecular data. Examples are given for hydrogen plasmas and plasmas with hydrocarbons which both are of importance in industrial applications as well as in fusion experiments.

Molecular Diagnostics of Fusion and Laboratory Plasmas

International Nuclear Information System (INIS)

Fantz, U.

2005-01-01

The presence of molecules in the cold scrape-off layer of fusion experiments and industrial plasmas requires an understanding of the molecular dynamics in these low temperature plasmas. Suitable diagnostic methods can provide an insight in molecular processes in the plasma volume as well as for plasma surface interactions. A very simple but powerful technique is the molecular emission spectroscopy. Spectra are obtained easily, whereas interpretation might be very complex and relies on the availability of atomic and molecular data. Examples are given for hydrogen plasmas and plasmas with hydrocarbons which both are of importance in industrial applications as well as in fusion experiments

Biophysical and molecular docking insight into the interaction of cytosine β-D arabinofuranoside with human serum albumin

Energy Technology Data Exchange (ETDEWEB)

Alam, Parvez; Chaturvedi, Sumit Kumar [Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, UP (India); Anwar, Tamanna [Center of Bioinformatics Research and Technology, Aligarh 202002 (India); Siddiqi, Mohammad Khursheed; Ajmal, Mohd Rehan [Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, UP (India); Badr, Gamal [Laboratory of Immunology & Molecular Physiology, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut (Egypt); Mahmoud, Mohamed H. [Food Science and Nutrition Department, National Research Center, Dokki, Cairo (Egypt); Deanship of Scientific Research, King Saud University, Riyadh (Saudi Arabia); Hasan Khan, Rizwan, E-mail: rizwanhkhan@hotmail.com [Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, UP (India)

2015-08-15

Interaction of pharmacologically important anticancer drug cytosine β-D arabinofuranoside with human serum albumin (HSA) at physiological pH 7.4 has been studied by utilizing various spectroscopic and molecular docking strategies. Fluorescence results revealed that cytosine β-D arabinofuranoside interacts with HSA through static quenching mechanism with binding affinity of 2.4×10{sup 3} M{sup −1}. The average binding distance between drug and Trp{sup 214} of HSA was found to be 2.23 nm on the basis of the theory of Förster's energy transfer. Synchronous fluorescence data indicated that interaction of drug with HSA changed the microenvironment around the tryptophan residue. UV–visible spectroscopy and circular dichroism results deciphered the complex formation and conformational alterations in the HSA respectively. Dynamic light scattering was utilized to understand the topology of protein in absence and presence of drug. Thermodynamic parameters obtained from isothermal titration calorimetry (ΔH=−26.01 kJ mol{sup −1} and TΔS=6.5 kJ mol{sup −1}) suggested the involvement of van der Waal interaction and hydrogen bonding. Molecular docking and displacement study with site specific markers suggested that cytosine β-D arabinofuranoside binds to subdomain IB of HSA which is also known as the hemin binding site. This study will be helpful to understand the binding mechanism of cytosine β-D arabinofuranoside with HSA and associated alterations. - Highlights: • Comprehensive insight into the interaction of CBDA with HSA. • The interaction process is spontaneous and exothermic. • The main governing forces for stabilizing HSA–CBDA complex are van der Waal interaction and hydrogen bonding. • CBDA binds at subdomain IB on HSA.

Computational Modelling of Dapsone Interaction With Dihydropteroate Synthase in Mycobacterium leprae; Insights Into Molecular Basis of Dapsone Resistance in Leprosy.

Science.gov (United States)

Chaitanya V, Sundeep; Das, Madhusmita; Bhat, Pritesh; Ebenezer, Mannam

2015-10-01

The molecular basis for determination of resistance to anti-leprosy drugs is the presence of point mutations within the genes of Mycobacterium leprae (M. leprae) that encode active drug targets. The downstream structural and functional implications of these point mutations on drug targets were scarcely studied. In this study, we utilized computational tools to develop native and mutant protein models for 5 point mutations at codon positions 53 and 55 in 6-hydroxymethyl-7, 8-dihydropteroate synthase (DHPS) of M. leprae, an active target for dapsone encoded by folp1 gene, that confer resistance to dapsone. Molecular docking was performed to identify variations in dapsone interaction with mutant DHPS in terms of hydrogen bonding, hydrophobic interactions, and energy changes. Schrodinger Suite 2014-3 was used to build homology models and in performing molecular docking. An increase in volume of the binding cavities of mutant structures was noted when compared to native form indicating a weakening in interaction (60.7 Å(3) in native vs. 233.6 Å(3) in Thr53Ala, 659.9 Å(3) in Thr53Ile, 400 Å(3) for Thr53Val, 385 Å(3) for Pro55Arg, and 210 Å(3) for Pro55Leu). This was also reflected by changes in hydrogen bonds and decrease in hydrophobic interactions in the mutant models. The total binding energy (ΔG) decreased significantly in mutant forms when compared to the native form (-51.92 Kcal/mol for native vs. -35.64, -35.24, -46.47, -47.69, and -41.36 Kcal/mol for mutations Thr53Ala, Thr53Ile, Thr53Val, Pro55Arg, and Pro55Leu, respectively. In brief, this analysis provided structural and mechanistic insights to the degree of dapsone resistance contributed by each of these DHPS mutants in leprosy. © 2015 Wiley Periodicals, Inc.

The transcriptome of Trichuris suis--first molecular insights into a parasite with curative properties for key immune diseases of humans.

Directory of Open Access Journals (Sweden)

Cinzia Cantacessi

Full Text Available Iatrogenic infection of humans with Trichuris suis (a parasitic nematode of swine is being evaluated or promoted as a biological, curative treatment of immune diseases, such as inflammatory bowel disease (IBD and ulcerative colitis, in humans. Although it is understood that short-term T. suis infection in people with such diseases usually induces a modified Th2-immune response, nothing is known about the molecules in the parasite that induce this response.As a first step toward filling the gaps in our knowledge of the molecular biology of T. suis, we characterised the transcriptome of the adult stage of this nematode employing next-generation sequencing and bioinformatic techniques. A total of ∼65,000,000 reads were generated and assembled into ∼20,000 contiguous sequences ( = contigs; ∼17,000 peptides were predicted and classified based on homology searches, protein motifs and gene ontology and biological pathway mapping.These analyses provided interesting insights into a number of molecular groups, particularly predicted excreted/secreted molecules (n = 1,288, likely to be involved in the parasite-host interactions, and also various molecules (n = 120 linked to chemokine, T-cell receptor and TGF-β signalling as well as leukocyte transendothelial migration and natural killer cell-mediated cytotoxicity, which are likely to be immuno-regulatory or -modulatory in the infected host. This information provides a conceptual framework within which to test the immunobiological basis for the curative effect of T. suis infection in humans against some immune diseases. Importantly, the T. suis transcriptome characterised herein provides a curated resource for detailed studies of the immuno-molecular biology of this parasite, and will underpin future genomic and proteomic explorations.

Molecular Features of Humic Acids and Fulvic Acids from Contrasting Environments

NARCIS (Netherlands)

Schellekens, Judith; Buurman, Peter; Kalbitz, Karsten; Zomeren, van Andre; Vidal-Torrado, Pablo; Cerli, Chiara; Comans, Rob N.J.

2017-01-01

Insight in the molecular structure of humic acid (HA) and fulvic acid (FA) can contribute to identify relationships between their molecular properties, and further our quantitative abilities to model important organic matter functions such as metal complexation and association with mineral

Precambrian Surface Temperatures and Molecular Phylogeny

Science.gov (United States)

Schwartzman, David; Lineweaver, Charles H.

2004-06-01

The timing of emergence of major organismal groups is consistent with the climatic temperature being equal to their upper temperature limit of growth (T_{max}), implying a temperature constraint on the evolution of each group, with the climatic temperature inferred from the oxygen isotope record of marine cherts. Support for this constraint comes from the correlation of T_{max} with the rRNA molecular phylogenetic distance from the last common ancestor (LCA) for both thermophilic Archaea and Bacteria. In particular, this correlation for hyperthermophilic Archaea suggests a climatic temperature of about 120°C at the time of the LCA, likely in the Hadean.

Computer-Based Molecular Modelling: Finnish School Teachers' Experiences and Views

Science.gov (United States)

Aksela, Maija; Lundell, Jan

2008-01-01

Modern computer-based molecular modelling opens up new possibilities for chemistry teaching at different levels. This article presents a case study seeking insight into Finnish school teachers' use of computer-based molecular modelling in teaching chemistry, into the different working and teaching methods used, and their opinions about necessary…

Multidisciplinary approaches to climate change questions

Science.gov (United States)

Middleton, Beth A.; LePage, Ben A.

2011-01-01

Multidisciplinary approaches are required to address the complex environmental problems of our time. Solutions to climate change problems are good examples of situations requiring complex syntheses of ideas from a vast set of disciplines including science, engineering, social science, and the humanities. Unfortunately, most ecologists have narrow training, and are not equipped to bring their environmental skills to the table with interdisciplinary teams to help solve multidisciplinary problems. To address this problem, new graduate training programs and workshops sponsored by various organizations are providing opportunities for scientists and others to learn to work together in multidisciplinary teams. Two examples of training in multidisciplinary thinking include those organized by the Santa Fe Institute and Dahlem Workshops. In addition, many interdisciplinary programs have had successes in providing insight into climate change problems including the International Panel on Climate Change, the Joint North American Carbon Program, the National Academy of Science Research Grand Challenges Initiatives, and the National Academy of Science. These programs and initiatives have had some notable success in outlining some of the problems and solutions to climate change. Scientists who can offer their specialized expertise to interdisciplinary teams will be more successful in helping to solve the complex problems related to climate change.

Molecular Population Genetics.

Science.gov (United States)

Casillas, Sònia; Barbadilla, Antonio

2017-03-01

Molecular population genetics aims to explain genetic variation and molecular evolution from population genetics principles. The field was born 50 years ago with the first measures of genetic variation in allozyme loci, continued with the nucleotide sequencing era, and is currently in the era of population genomics. During this period, molecular population genetics has been revolutionized by progress in data acquisition and theoretical developments. The conceptual elegance of the neutral theory of molecular evolution or the footprint carved by natural selection on the patterns of genetic variation are two examples of the vast number of inspiring findings of population genetics research. Since the inception of the field, Drosophila has been the prominent model species: molecular variation in populations was first described in Drosophila and most of the population genetics hypotheses were tested in Drosophila species. In this review, we describe the main concepts, methods, and landmarks of molecular population genetics, using the Drosophila model as a reference. We describe the different genetic data sets made available by advances in molecular technologies, and the theoretical developments fostered by these data. Finally, we review the results and new insights provided by the population genomics approach, and conclude by enumerating challenges and new lines of inquiry posed by increasingly large population scale sequence data. Copyright © 2017 Casillas and Barbadilla.

Economic development, climate and values: making policy.

Science.gov (United States)

Stern, Nicholas

2015-08-07

The two defining challenges of this century are overcoming poverty and managing the risks of climate change. Over the past 10 years, we have learned much about how to tackle them together from ideas on economic development and public policy. My own work in these areas over four decades as an academic and as a policy adviser in universities and international financial institutions has focused on how the investment environment and the empowerment of people can change lives and livelihoods. The application of insights from economic development and public policy to climate change requires rigorous analysis of issues such as discounting, modelling the risks of unmanaged climate change, climate policy targets and estimates of the costs of mitigation. The latest research and results show that the case for avoiding the risks of dangerous climate change through the transition to low-carbon economic development and growth is still stronger than when the Stern Review was published. This is partly because of evidence that some of the impacts of climate change are happening more quickly than originally expected, and because of remarkable advances in technologies, such as solar power. Nevertheless, significant hurdles remain in securing the international cooperation required to avoid dangerous climate change, not least because of disagreements and misunderstandings about key issues, such as ethics and equity. © 2015 The Author(s).

Do it well and do it right: The impact of service climate and ethical climate on business performance and the boundary conditions.

Science.gov (United States)

Jiang, Kaifeng; Hu, Jia; Hong, Ying; Liao, Hui; Liu, Songbo

2016-11-01

Prior research has demonstrated that service climate can enhance unit performance by guiding employees' service behavior to satisfy customers. Extending this literature, we identified ethical climate toward customers as another indispensable organizational climate in service contexts and examined how and when service climate operates in conjunction with ethical climate to enhance business performance of service units. Based on data collected in 2 phases over 6 months from multiple sources of 196 movie theaters, we found that service climate and ethical climate had disparate impacts on business performance, operationalized as an index of customer attendance rate and operating income per labor hour, by enhancing service behavior and reducing unethical behavior, respectively. Furthermore, we found that service behavior and unethical behavior interacted to affect business performance, in such a way that service behavior was more positively related to business performance when unethical behavior was low than when it was high. This interactive effect between service and unethical behaviors was further strengthened by high market turbulence and competitive intensity. These findings provide new insight into theoretical development of service management and offer practical implications about how to maximize business performance of service units by managing organizational climates and employee behaviors synergistically. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

New insights into the molecular mechanism of intestinal fatty acid absorption.

Science.gov (United States)

Wang, Tony Y; Liu, Min; Portincasa, Piero; Wang, David Q-H

2013-11-01

Dietary fat is one of the most important energy sources of all the nutrients. Fatty acids, stored as triacylglycerols (also called triglycerides) in the body, are an important reservoir of stored energy and derived primarily from animal fats and vegetable oils. Although the molecular mechanisms for the transport of water-insoluble amphipathic fatty acids across cell membranes have been debated for many years, it is now believed that the dominant means for intestinal fatty acid uptake is via membrane-associated fatty acid-binding proteins, that is, fatty acid transporters on the apical membrane of enterocytes. These findings indicate that intestinal fatty acid absorption is a multistep process that is regulated by multiple genes at the enterocyte level, and intestinal fatty acid absorption efficiency could be determined by factors influencing intraluminal fatty acid molecules across the brush border membrane of enterocytes. To facilitate research on intestinal, hepatic and plasma triacylglycerol metabolism, it is imperative to establish standard protocols for precisely and accurately measuring the efficiency of intestinal fatty acid absorption in humans and animal models. In this review, we will discuss the chemical structure and nomenclature of fatty acids and summarize recent progress in investigating the molecular mechanisms underlying the intestinal absorption of fatty acids, with a particular emphasis on the physical chemistry of intestinal lipids and the molecular physiology of intestinal fatty acid transporters. A better understanding of the molecular mechanism of intestinal fatty acid absorption should lead to novel approaches to the treatment and the prevention of fatty acid-related metabolic diseases that are prevalent worldwide. © 2013 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd.

An Analysis of the Climate Data Initiative's Data Collection

Science.gov (United States)

Ramachandran, R.; Bugbee, K.

2015-12-01

The Climate Data Initiative (CDI) is a broad multi-agency effort of the U.S. government that seeks to leverage the extensive existing federal climate-relevant data to stimulate innovation and private-sector entrepreneurship to support national climate-change preparedness. The CDI project is a systematic effort to manually curate and share openly available climate data from various federal agencies. To date, the CDI has curated seven themes, or topics, relevant to climate change resiliency. These themes include Coastal Flooding, Food Resilience, Water, Ecosystem Vulnerability, Human Health, Energy Infrastructure, and Transportation. Each theme was curated by subject matter experts who selected datasets relevant to the topic at hand. An analysis of the entire Climate Data Initiative data collection and the data curated for each theme offers insights into which datasets are considered most relevant in addressing climate resiliency. Other aspects of the data collection will be examined including which datasets were the most visited or popular and which datasets were the most sought after for curation by the theme teams. Results from the analysis of the CDI collection will be presented in this talk.

Getting Smart? Climate Change and the Electric Grid

Directory of Open Access Journals (Sweden)

James Meadowcroft

2013-09-01

Full Text Available Interest in the potential of smart grid to transform the way societies generate, distribute, and use electricity has increased dramatically over the past decade. A smarter grid could contribute to both climate change mitigation and adaptation by increasing low-carbon electricity production and enhancing system reliability and resilience. However, climate goals are not necessarily essential for smart grid. Climate change is only one of many considerations motivating innovation in electricity systems, and depending on the path of grid modernization, a future smart grid might do little to reduce, or could even exacerbate, risks associated with climate change. This paper identifies tensions within a shared smart grid vision and illustrates how competing societal priorities are influencing electricity system innovation. Co-existing but divergent priorities among key actors’ are mapped across two critical dimensions: centralized versus decentralized energy systems and radical versus incremental change. Understanding these tensions provides insights on how climate change objectives can be integrated to shape smart grid development. Electricity system change is context-specific and path-dependent, so specific strategies linking smart grid and climate change need to be developed at local, regional, and national levels. And while incremental improvements may bring short term gains, a radical transformation is needed to realize climate objectives.

Modelling interactions of carbon dioxide, forests, and climate

International Nuclear Information System (INIS)

Luxmoore, R.J.; Baldocchi, D.D.

1994-01-01

Atmospheric carbon dioxide is rising and forests and climate is changing exclamation point This combination of fact and premise may be evaluated at a range of temporal and spatial scales with the aid of computer simulators describing the interrelationships between forest vegetation, litter and soil characteristics, and appropriate meteorological variables. Some insights on the effects of climate on the transfers of carbon and the converse effect of carbon transfer on climate are discussed as a basis for assessing the significance of feedbacks between vegetation and climate under conditions of rising atmospheric carbon dioxide. Three main classes of forest models are reviewed. These are physiologically-based models, forest succession simulators based on the JABOWA model, and ecosystem-carbon budget models that use compartment transfer rates with empirically estimated coefficients. Some regression modeling approaches are also outlined. Energy budget models applied to forests and grasslands are also reviewed. This review presents examples of forest models; a comprehensive discussion of all available models is not undertaken

Climate change. Managing the risks

International Nuclear Information System (INIS)

Swart, R.J.

1994-01-01

In order to address the key question if a targeted approach to climate change response is feasible, different aspects of this question are analyzed. First, the scientific and political aspects of different options to determine specific long-term objectives for climate change are evaluated on the basis of the current scientific insights and the experiences over the last 5 years to develop climate objectives. Preliminary directions for such objectives are given. Next, important analytical tools are discussed that can be applied to analyze the different options and their implications in detail. In order to evaluate the implications of mitigation options, strategies that are consistent with the preliminary climate goals are analyzed in the third part. In chapter 2, the concept of long-term environmental goals, derived from critical levels of climate change, is discussed. Also a historical perspective is provided. A new, systematic regionalized and risk-based approach to elaborate the ultimate objective of the Framework Convention on Climate Change is proposed. In chapter 3 scenarios and integrated models are discussed. Central is the description of scenarios that were developed with RlVM's Integrated Model to Assess the Greenhouse Effect (IMAGE) and the US-EPA's Atmospheric Stabilization Framework (ASF). In chapter 4 potential long-term international emissions control strategies for the different sources and sinks of the most important greenhouse gases are analyzed. Carbon dioxide from energy, carbon dioxide from deforestation, and non-CO 2 greenhouse gases are dealt with subsequently. The dissertation ends with general conclusions and recommendations for the further design of a targeted approach to climate change response, the development of analytical tools to support policy development in the area of climate change, and strategies that are consistent with preliminary long-term environmental goals. 66 figs., 8 tabs., 417 refs., 1 appendix

Molecularly targeted therapeutic radiopharmaceuticals

International Nuclear Information System (INIS)

Saw, M.M.

2007-01-01

Full text: It is generally agreed that current focus of nuclear medicine development should be on molecular imaging and therapy. Though, the widespread use of the terminology 'molecular imaging' is quite recent, nuclear medicine has used molecular imaging techniques for more than 20 years ago. A variety of radiopharmaceuticals have been introduced for the internal therapy of malignant and inflammatory lesions in nuclear medicine. In the field of bio/medical imaging, nuclear medicine is one of the disciplines which has the privilege of organized and well developed chemistry/ pharmacy section; radio-chemistry/radiopharmacy. Fundamental principles have been developed more than 40 years ago and advanced research is going well into postgenomic era. The genomic revolution and dramatically increased insight in the molecular mechanisms underlying pathology have led to paradigm shift in drug development. Likewise does in the nuclear medicine. Here, the author will present current clinical and pre-clinical therapeutic radiopharmaceuticals based on molecular targets such as membrane-bound receptors, enzymes, nucleic acids, sodium iodide symporter, etc, in correlation with fundamentals of radiopharmacy. (author)

Climate change, water stress, conflict and migration : Taking stock of current insights through a vulnerability lens

NARCIS (Netherlands)

Hermans, L.M.

2011-01-01

There is a growing awareness in international policy circles that climate change may be a driver of increased migration flows. In addition to political refugees and economic migrants, climate change-induced migration and environmental migrants are increasingly recognized as categories in human

New insights into the metastatic behavior after breast cancer surgery, according to well-established clinicopathological variables and molecular subtypes.

Directory of Open Access Journals (Sweden)

Oreste Claudio Buonomo

Full Text Available Despite advances in treatment, up to 30% of patients with early breast cancer (BC experience distant disease relapse. However, a comprehensive understanding of tumor spread and site-specific recurrence patterns remains lacking. This retrospective case-control study included 103 consecutive patients with metastatic BC admitted to our institution (2000-2013. Cases were matched according to age, tumor biology, and clinicopathological features to 221 patients with non-metastatic BC (control group. The median follow-up period among the 324 eligible patients was 7.3 years. While relatively low values for sensitivity (71% and specificity (56% were found for axillary lymph node (ALN involvement as an indicator of risk and pattern of distant relapse, nodal status remained the most powerful predictor of metastases (OR: 3.294; CL: 1.9-5.5. Rates of dissemination and metastatic efficiency differed according to molecular subtype. HER2-positive subtypes showed a stronger association with systemic spread (OR: 2.127; CL: 1.2-3.8 than other subgroups. Classification as Luminal or Non-Luminal showed an increased risk of lung and distant nodal recurrence, and a decreased risk in bone metastases in the Non-Luminal group (OR: 2.9, 3.345, and 0.2, respectively. Tumors with HER2 overexpression had a significantly high risk for distant relapse (OR: 2.127 compared with HER2-negative tumors and also showed higher central nervous system (CNS and lung metastatic potential (OR: 5.6 and 2.65, respectively and low risk of bone disease progression (OR: 0.294. Furthermore, we found significant associations between biological profiles and sites of recurrence. A new process of clinical/diagnostic staging, including molecular subtypes, could better predict the likelihood of distant relapses and their anatomical location. Recognition and appreciation of clinically distinct molecular subtypes may assist in evaluation of the probability of distant relapses and their sites. Our

Development and nationwide scale-up of Climate Matters, a localized climate change education program delivered by TV weathercasters.

Science.gov (United States)

Cullen, H. M.; Maibach, E.

2016-12-01

its development and national scale-up, and conclude with insights for how to develop climate communication initiatives for other professional communities of practice in the U.S. and other countries.

Structural and molecular insights into novel surface-exposed mucus adhesins from Lactobacillus reuteri human strains.

Science.gov (United States)

Etzold, Sabrina; MacKenzie, Donald A; Jeffers, Faye; Walshaw, John; Roos, Stefan; Hemmings, Andrew M; Juge, Nathalie

2014-05-01

The mucus layer covering the gastrointestinal tract is the first point of contact of the intestinal microbiota with the host. Cell surface macromolecules are critical for adherence of commensal bacteria to mucus but structural information is scarce. Here we report the first molecular and structural characterization of a novel cell-surface protein, Lar_0958 from Lactobacillus reuteri JCM 1112(T) , mediating adhesion of L. reuteri human strains to mucus. Lar_0958 is a modular protein of 133 kDa containing six repeat domains, an N-terminal signal sequence and a C-terminal anchoring motif (LPXTG). Lar_0958 homologues are expressed on the cell-surface of L. reuteri human strains, as shown by flow-cytometry and immunogold microscopy. Adhesion of human L. reuteri strains to mucus in vitro was significantly reduced in the presence of an anti-Lar_0958 antibody and Lar_0958 contribution to adhesion was further confirmed using a L. reuteri ATCC PTA 6475 lar_0958 KO mutant (6475-KO). The X-ray crystal structure of a single Lar_0958 repeat, determined at 1.5 Å resolution, revealed a divergent immunoglobulin (Ig)-like β-sandwich fold, sharing structural homology with the Ig-like inter-repeat domain of internalins of the food borne pathogen Listeria monocytogenes. These findings provide unique structural insights into cell-surface protein repeats involved in adhesion of Gram-positive bacteria to the intestine. © 2014 John Wiley & Sons Ltd.

Climate adaption and post-fire restoration of a foundational perennial in cold desert: Insights from intraspecific variation in response to weather

Science.gov (United States)

Brabec, Martha M.; Germino, Matthew; Richardson, Bryce A.

2017-01-01

responses among subspecies/cytotypes were not as strong and did not relate to survival patterns. 5.Synthesis and applications. Low temperature responses are a key axis defining climate adaptation in young sagebrush seedlings and vary more with cytotype than with subspecies, which contrasts with the traditional emphases on (i) water limitations to explain establishment in these deserts, and (ii) subspecies in selecting restoration seedings. These important and novel insights on climate adaptation are critical for seed selection and parameterizing seed transfer zones, and were made possible by incorporating weather data with survival statistics. The survival/weather statistics used here could be applied to any restoration planting or seeding to help elucidate factors contributing to success and enable adaptive management.

Smallholder agriculture in India and adaptation to current and future climate variability and climate change

Science.gov (United States)

Murari, K. K.; Jayaraman, T.

2014-12-01

of climate variability on smallholder agriculture in the present can therefore provide important insights into the nature of its vulnerability to future climate change.

Holocene Glacier Fluctuations in the Peruvian Andes Indicate Northern Climate Linkages

Science.gov (United States)

Licciardi, Joseph M.; Schaefer, Joerg M.; Taggart, Jean R.; Lund, David C.

2009-09-01

The role of the tropics in triggering, transmitting, and amplifying interhemispheric climate signals remains a key debate in paleoclimatology. Tropical glacier fluctuations provide important insight on regional paleoclimatic trends and forcings, but robust chronologies are scarce. Here, we report precise moraine ages from the Cordillera Vilcabamba (13°20‧S) of southern Peru that indicate prominent glacial events and associated climatic shifts in the outer tropics during the early Holocene and late in the “Little Ice Age” period. Our glacier chronologies differ from the New Zealand record but are broadly correlative with well-dated glacial records in Europe, suggesting climate linkages between the tropics and the North Atlantic region.

Molecular docking and molecular dynamics simulation study of inositol phosphorylceramide synthase – inhibitor complex in leishmaniasis: Insight into the structure based drug design [version 2; referees: 2 approved

Directory of Open Access Journals (Sweden)

Vineetha Mandlik

2016-09-01

Full Text Available Inositol phosphorylceramide synthase (IPCS has emerged as an important, interesting and attractive target in the sphingolipid metabolism of Leishmania. IPCS catalyzes the conversion of ceramide to IPC which forms the most predominant sphingolipid in Leishmania. IPCS has no mammalian equivalent and also plays an important role in maintaining the infectivity and viability of the parasite. The present study explores the possibility of targeting IPCS; development of suitable inhibitors for the same would serve as a treatment strategy for the infectious disease leishmaniasis. Five coumarin derivatives were developed as inhibitors of IPCS protein. Molecular dynamics simulations of the complexes of IPCS with these inhibitors were performed which provided insights into the binding modes of the inhibitors. In vitro screening of the top three compounds has resulted in the identification of one of the compounds (compound 3 which shows little cytotoxic effects. This compound therefore represents a good starting point for further in vivo experimentation and could possibly serve as an important drug candidate for the treatment of leishmaniasis.

Climate Change Impacts and Responses: Societal Indicators for the National Climate Assessment

Science.gov (United States)

Kenney, Melissa A.; Chen, Robert S.; Maldonado, Julie; Quattrochi, Dale

2011-01-01

The Climate Change Impacts and Responses: Societal Indicators for the National Climate Assessment workshop, sponsored by the National Aeronautics and Space Administration (NASA) for the National Climate Assessment (NCA), was held on April 28-29, 2011 at The Madison Hotel in Washington, DC. A group of 56 experts (see list in Appendix B) convened to share their experiences. Participants brought to bear a wide range of disciplinary expertise in the social and natural sciences, sector experience, and knowledge about developing and implementing indicators for a range of purposes. Participants included representatives from federal and state government, non-governmental organizations, tribes, universities, and communities. The purpose of the workshop was to assist the NCA in developing a strategic framework for climate-related physical, ecological, and socioeconomic indicators that can be easily communicated with the U.S. population and that will support monitoring, assessment, prediction, evaluation, and decision-making. The NCA indicators are envisioned as a relatively small number of policy-relevant integrated indicators designed to provide a consistent, objective, and transparent overview of major variations in climate impacts, vulnerabilities, adaptation, and mitigation activities across sectors, regions, and timeframes. The workshop participants were asked to provide input on a number of topics, including: (1) categories of societal indicators for the NCA; (2) alternative approaches to constructing indicators and the better approaches for NCA to consider; (3) specific requirements and criteria for implementing the indicators; and (4) sources of data for and creators of such indicators. Socioeconomic indicators could include demographic, cultural, behavioral, economic, public health, and policy components relevant to impacts, vulnerabilities, and adaptation to climate change as well as both proactive and reactive responses to climate change. Participants provided

Introducing an integrated climate change perspective in POPs modelling, monitoring and regulation

International Nuclear Information System (INIS)

Lamon, L.; Dalla Valle, M.; Critto, A.; Marcomini, A.

2009-01-01

This paper presents a review on the implications of climate change on the monitoring, modelling and regulation of persistent organic pollutants (POPs). Current research gaps are also identified and discussed. Long-term data sets are essential to identify relationships between climate fluctuations and changes in chemical species distribution. Reconstructing the influence of climatic changes on POPs environmental behaviour is very challenging in some local studies, and some insights can be obtained by the few available dated sediment cores or by studying POPs response to inter-annual climate fluctuations. Knowledge gaps and future projections can be studied by developing and applying various modelling tools, identifying compounds susceptibility to climate change, local and global effects, orienting international policies. Long-term monitoring strategies and modelling exercises taking into account climate change should be considered when devising new regulatory plans in chemicals management. - Climate change implications on POPs are addressed here with special attention to monitoring, modelling and regulation issues.

Molecular diagnostics of neurodegenerative disorders

Directory of Open Access Journals (Sweden)

Megha eAgrawal

2015-09-01

Full Text Available Molecular diagnostics provide a powerful method to detect and diagnose various neurological diseases such as Alzheimer’s and Parkinson’s disease. The confirmation of such diagnosis allows early detection and subsequent medical counseling that help specific patients to undergo clinically important drug trials. This provides a medical pathway to have better insight of neurogenesis and eventual cure of the neurodegenerative diseases. In this short review, we present recent advances in molecular diagnostics especially biomarkers and imaging spectroscopy for neurological diseases. We describe advances made in Alzheimer’s disease, Parkinson’s disease, Amyotrophic lateral sclerosis and Huntington’s disease, and finally present a perspective on the future directions to provide a framework for further developments and refinements of molecular diagnostics to combat neurodegenerative disorders.

Molecular diagnostics of neurodegenerative disorders.

Science.gov (United States)

Agrawal, Megha; Biswas, Abhijit

2015-01-01

Molecular diagnostics provide a powerful method to detect and diagnose various neurological diseases such as Alzheimer's and Parkinson's disease. The confirmation of such diagnosis allows early detection and subsequent medical counseling that help specific patients to undergo clinically important drug trials. This provides a medical pathway to have better insight of neurogenesis and eventual cure of the neurodegenerative diseases. In this short review, we present recent advances in molecular diagnostics especially biomarkers and imaging spectroscopy for neurological diseases. We describe advances made in Alzheimer's disease (AD), Parkinson's disease (PD), Amyotrophic lateral sclerosis (ALS) and Huntington's disease (HD), and finally present a perspective on the future directions to provide a framework for further developments and refinements of molecular diagnostics to combat neurodegenerative disorders.

Molecular and genetic insights into an infantile epileptic encephalopathy-CDKL5 disorder

Institute of Scientific and Technical Information of China (English)

Ailing Zhou; Song Han; Zhaolan Joe Zhou

2017-01-01

BACKGROUND:The discovery that mutations in cyclin-dependent kinase-like 5 (CDKL5) gene are associated with infantile epileptic encephalopathy has stimulated world-wide research effort to understand the molecular and genetic basis of CDKL5 disorder.Given the large number of literature published thus far,this review aims to summarize current genetic studies,draw a consensus on proposed molecular functions,and point to gaps of knowledge in CDKL5 research.METHODS:A systematic review process was conducted using the PubMed search engine focusing on CDKL5 studies in the recent ten years.We analyzed these publications and summarized the findings into four sections:genetic studies,CDKL5 expression pattems,molecular functions,and animal models.We also discussed challenges and future directions in each section.RESULTS:On the clinical side,CDKL5 disorder is characterized by early onset epileptic seizures,intellectual disability,and stereotypical behaviors.On the research side,a series of molecular and genetic studies in human patients,cell cultures and animal models have established the causality of CDKL5 to the infantile epileptic encephalopathy,and pointed to a key role for CDKL5 in regulating neuronal function in the brain.Mouse models of CDKL5 disorder have also been developed,and notably,manifest behavioral phenotypes,mimicking numerous clinical symptoms of CDKL5 disorder and advancing CDKL5 research to the preclinical stage.CONCLUSIONS:Given what we have leamed thus far,future identification of robust,quantitative,and sensitive outcome measures would be the key in animal model studies,particularly in heterozygous females.In the meantime,molecular and cellular studies of CDKL5 should focus on mechanism-based investigation and aim to uncover druggable targets that offer the potential to rescue or ameliorate CDKL5 disorder-related phenotypes.

Molecular and genetic insights into an infantile epileptic encephalopathy - CDKL5 disorder.

Science.gov (United States)

Zhou, Ailing; Han, Song; Zhou, Zhaolan Joe

2017-02-01

The discovery that mutations in cyclin-dependent kinase-like 5 ( CDKL5 ) gene are associated with infantile epileptic encephalopathy has stimulated world-wide research effort to understand the molecular and genetic basis of CDKL5 disorder. Given the large number of literature published thus far, this review aims to summarize current genetic studies, draw a consensus on proposed molecular functions, and point to gaps of knowledge in CDKL5 research. A systematic review process was conducted using the PubMed search engine focusing on CDKL5 studies in the recent ten years. We analyzed these publications and summarized the findings into four sections: genetic studies, CDKL5 expression patterns, molecular functions, and animal models. We also discussed challenges and future directions in each section. On the clinical side, CDKL5 disorder is characterized by early onset epileptic seizures, intellectual disability, and stereotypical behaviors. On the research side, a series of molecular and genetic studies in human patients, cell cultures and animal models have established the causality of CDKL5 to the infantile epileptic encephalopathy, and pointed to a key role for CDKL5 in regulating neuronal function in the brain. Mouse models of CDKL5 disorder have also been developed, and notably, manifest behavioral phenotypes, mimicking numerous clinical symptoms of CDKL5 disorder and advancing CDKL5 research to the preclinical stage. Given what we have learned thus far, future identification of robust, quantitative, and sensitive outcome measures would be the key in animal model studies, particularly in heterozygous females. In the meantime, molecular and cellular studies of CDKL5 should focus on mechanism-based investigation and aim to uncover druggable targets that offer the potential to rescue or ameliorate CDKL5 disorder-related phenotypes.

Molecular taxonomy provides new insights into anopheles species of the neotropical arribalzagia series.

Directory of Open Access Journals (Sweden)

Giovan F Gómez

Full Text Available Phylogenetic analysis of partial mitochondrial cytochrome oxidase c subunit I (COI and nuclear internal transcribed spacer 2 (ITS2 sequences were used to evaluate initial identification and to investigate phylogenetic relationships of seven Anopheles morphospecies of the Arribalzagia Series from Colombia. Phylogenetic trees recovered highly supported clades for An. punctimaculas.s., An. calderoni, An. malefactor s.l., An. neomaculipalpus, An. apicimacula s.l., An. mattogrossensis and An. peryassui. This study provides the first molecular confirmation of An. malefactorfrom Colombia and discovered conflicting patterns of divergence for the molecular markers among specimens from northeast and northern Colombia suggesting the presence of two previously unrecognized Molecular Operational Taxonomic Units (MOTUs. Furthermore, two highly differentiated An. apicimacula MOTUs previously found in Panama were detected. Overall, the combined molecular dataset facilitated the detection of known and new Colombian evolutionary lineages, and constitutes the baseline for future research on their bionomics, ecology and potential role as malaria vectors.

Improving the effectiveness of communication about climate science: Insights from the "Global Warming's Six Americas" audience segmentation research project

Science.gov (United States)

Maibach, E.; Roser-Renouf, C.

2011-12-01

That the climate science community has not been entirely effective in sharing what it knows about climate change with the broader public - and with policy makers and organizations that should be considering climate change when making decisions - is obvious. Our research shows that a large majority of the American public trusts scientists (76%) and science-based agencies (e.g., 76% trust NOAA) as sources of information about climate change. Yet, despite the widespread agreement in the climate science community that the climate is changing as a result of human activity, only 64% of the public understand that the world's average temperature has been increasing (and only about half of them are sure), less than half (47%) understand that the warming is caused mostly by human activity, and only 39% understand that most scientists think global warming is happening (in fact, only 13% understand that the large majority of climate scientists think global warming is happening). Less obvious is what the climate science community should do to become more effective in sharing what it knows. In this paper, we will use evidence from our "Global Warming's Six Americas" audience segmentation research project to suggest ways that individual climate scientists -- and perhaps more importantly, ways in which climate science agencies and professional societies -- can enhance the effectiveness of their communication efforts. We will conclude by challenging members of the climate science community to identify and convey "simple, clear messages, repeated often, by a variety of trusted sources" - an approach to communication repeatedly shown to be effective by the public health community.

Deep time evidence for climate sensitivity increase with warming

DEFF Research Database (Denmark)

Shaffer, Gary; Huber, Matthew; Rondanelli, Roberto

2016-01-01

warming analogue. We obtain constrained estimates of CO2 and climate sensitivity before and during the PETM and of the PETM carbon input amount and nature. Sensitivity increased from 3.3-5.6 to 3.7-6.5K (Kelvin) into the PETM. When taken together with Last Glacial Maximum and modern estimates, this result...... world, but past warming events may provide insight. Here we employ paleoreconstructions and new climate-carbon model simulations in a novel framework to explore a wide scenario range for the Paleocene-Eocene Thermal Maximum (PETM) carbon release and global warming event 55.8Ma ago, a possible future...

Insights into the sequence parameters for halophilic adaptation.

Science.gov (United States)

Nath, Abhigyan

2016-03-01

The sequence parameters for halophilic adaptation are still not fully understood. To understand the molecular basis of protein hypersaline adaptation, a detailed analysis is carried out, and investigated the likely association of protein sequence attributes to halophilic adaptation. A two-stage strategy is implemented, where in the first stage a supervised machine learning classifier is build, giving an overall accuracy of 86 % on stratified tenfold cross validation and 90 % on blind testing set, which are better than the previously reported results. The second stage consists of statistical analysis of sequence features and possible extraction of halophilic molecular signatures. The results of this study showed that, halophilic proteins are characterized by lower average charge, lower K content, and lower S content. A statistically significant preference/avoidance list of sequence parameters is also reported giving insights into the molecular basis of halophilic adaptation. D, Q, E, H, P, T, V are significantly preferred while N, C, I, K, M, F, S are significantly avoided. Among amino acid physicochemical groups, small, polar, charged, acidic and hydrophilic groups are preferred over other groups. The halophilic proteins also showed a preference for higher average flexibility, higher average polarity and avoidance for higher average positive charge, average bulkiness and average hydrophobicity. Some interesting trends observed in dipeptide counts are also reported. Further a systematic statistical comparison is undertaken for gaining insights into the sequence feature distribution in different residue structural states. The current analysis may facilitate the understanding of the mechanism of halophilic adaptation clearer, which can be further used for rational design of halophilic proteins.

SOYBEAN - MOLECULAR ASPECTS OF BREEDING

Directory of Open Access Journals (Sweden)

Aleksandra Sudarić

2012-12-01

Full Text Available The book Soybean: Molecular Aspects of Breeding focuses recent progress in our understanding of the genetics and molecular biology of soybean. This book is divided into four parts and contains 22 chapters. Part I, Molecular Biology and Biotechnology focuses advances in molecular biology and laboratory procedures that have been developed recently to manipulate DNA. Part II, Breeding for abiotic stress covers proteomics approaches form as a powerful tool for investigating the molecular mechanisms of the plant responses to various types of abiotic stresses. Part III, Breeding for biotic stress addresses issues related to application of molecular based strategies in order to increase soybean resistance to various biotic factors. Part IV, Recent Technology reviews recent technologies into the realm of soybean monitoring, processing and product use. While the information accumulated in this book is of primary interest for plant breeders, valuable insights are also offered to agronomists, molecular biologists, physiologists, plant pathologists, food scientists and students. The book is a result of efforts made by many experts from different countries (USA, Japan, Croatia, Serbia, China, Canada, Malawi, Iran, Hong Kong, Brasil, Mexico.

Feasible Electricity Infrastructure Pathways in the Context of Climate-Water Change Constraints

Science.gov (United States)

Miara, A.; Vorosmarty, C. J.; Macknick, J.; Cohen, S. M.; Tidwell, V. C.; Newmark, R. L.; Fekete, B. M.; Corsi, F.; Sun, Y.; Proussevitch, A. A.; Glidden, S.

2017-12-01

The carbon and water intensity of US electricity generation has recently decreased due to the natural gas revolution and deployment of renewable technologies. Yet, power plants that require water for cooling still provide 80% of electricity generation and projected climate-water conditions may limit their power output and affect reliability. Understanding the connections and tradeoffs across water, electricity and climate systems is timely, as the nation tries to mitigate and adapt to a changing climate. Electricity expansion models are used to provide insight on power sector pathways given certain policy goals and economic conditions, but do not typically account for productivity limitations due to physical climate-water constraints. Here, we account for such constraints by coupling an electricity expansion model (Regional Energy Deployment System - ReEDS) with the combined Water Balance and Thermoelectric Power and Thermal Pollution Models (WBM-TP2M), which calculate the available capacity at power plants as a function of hydrologic flows, climate conditions, power plant technology and environmental regulations. To fully capture and incorporate climate-water impacts into ReEDS, a specific rule-set was designed for the temporal and spatial downscaling and up-scaling of ReEDS results into WBM-TP2M inputs and visa versa - required to achieve a modeling `loop' that will enable convergence on a feasible solution in the context of economic and geophysical constraints and opportunities. This novel modeling approach is the next phase of research for understanding electricity system vulnerabilities and adaptation measures using energy-water-climate modeling, which to-date has been limited by a focus on individual generators without analyzing power generation as a collective regional system. This study considers four energy policy/economic pathways under future climate-water resource conditions, designed under the National Energy Water System assessment framework. Results

Response of the mean global vegetation distribution to interannual climate variability

Energy Technology Data Exchange (ETDEWEB)

Notaro, Michael [University of Wisconsin-Madison, Center for Climatic Research, Madison, WI (United States)

2008-06-15

The impact of interannual variability in temperature and precipitation on global terrestrial ecosystems is investigated using a dynamic global vegetation model driven by gridded climate observations for the twentieth century. Contrasting simulations are driven either by repeated mean climatology or raw climate data with interannual variability included. Interannual climate variability reduces net global vegetation cover, particularly over semi-arid regions, and favors the expansion of grass cover at the expense of tree cover, due to differences in growth rates, fire impacts, and interception. The area burnt by global fires is substantially enhanced by interannual precipitation variability. The current position of the central United States' ecotone, with forests to the east and grasslands to the west, is largely attributed to climate variability. Among woody vegetation, climate variability supports expanded deciduous forest growth and diminished evergreen forest growth, due to difference in bioclimatic limits, leaf longevity, interception rates, and rooting depth. These results offer insight into future ecosystem distributions since climate models generally predict an increase in climate variability and extremes. (orig.)

Changes of the climate induced by trace gases. Current state of research

Energy Technology Data Exchange (ETDEWEB)

Grassl, H

1987-02-01

Any attempt of predicting climatic changes is a very different task, because it requires good insight into and knowledge of a complex system whose components respond very differently in terms of time. Hence there remain many uncertainties in efforts towards 'predicting' climatic changes considering the enhancing greenhouse effect. As with all environmental problems, the best reaction is to conserve resources, and systematically explore the interactions. Reducing the CO/sub 2/, for instance, will be highly awarded. (orig./HSCH).

Storm track response to climate change: Insights from simulations using an idealized dry GCM.

Science.gov (United States)

Mbengue, Cheikh; Schneider, Tapio

2013-04-01

The midlatitude storm tracks, where the most intense extratropical cyclones are found, are an important fixture in the general circulation. They are instrumental in balancing the Earth's heat, momentum, and moisture budgets and are responsible for the weather and climatic patterns over large regions of the Earth's surface. As a result, the midlatitude storm tracks are the subject of a considerable amount of scientific research to understand their response to global warming. This has produced the robust result showing that the storm tracks migrate poleward with global warming. However, the dynamical mechanisms responsible for this migration remain unclear. Our work seeks to broaden understanding of the dynamical mechanisms responsible for storm track migration. Competing mechanisms present in the comprehensive climate models often used to study storm track dynamics make it difficult to determine the primary mechanisms responsible for storm track migration. We are thus prompted to study storm track dynamics from a simplified and idealized framework, which enables the decoupling of mean temperature effects from the effects of static stability and of tropical from extratropical effects. Using a statistically zonally symmetric, dry general circulation model (GCM), we conduct a series of numerical simulations to help understand the storm track response to global mean temperatures and to the tropical convective static stability, which we can vary independently. We define storm tracks as regions of zonally and temporally averaged maxima of barotropic eddy kinetic energy (EKE). This storm track definition also allows us to use previously found scalings between the magnitude of bulk measures of mean available potential energy (MAPE) and EKE, to decompose MAPE, and to obtain some mechanistic understanding of the storm track response in our simulations. These simulations provide several insights, which enable us to extend upon existing theories on the mechanisms driving the

Climate Sensitivity, Sea Level, and Atmospheric Carbon Dioxide

Science.gov (United States)

Hansen, James; Sato, Makiko; Russell, Gary; Kharecha, Pushker

2013-01-01

Cenozoic temperature, sea level and CO2 covariations provide insights into climate sensitivity to external forcings and sea-level sensitivity to climate change. Climate sensitivity depends on the initial climate state, but potentially can be accurately inferred from precise palaeoclimate data. Pleistocene climate oscillations yield a fast-feedback climate sensitivity of 3+/-1deg C for a 4 W/sq m CO2 forcing if Holocene warming relative to the Last Glacial Maximum (LGM) is used as calibration, but the error (uncertainty) is substantial and partly subjective because of poorly defined LGM global temperature and possible human influences in the Holocene. Glacial-to-interglacial climate change leading to the prior (Eemian) interglacial is less ambiguous and implies a sensitivity in the upper part of the above range, i.e. 3-4deg C for a 4 W/sq m CO2 forcing. Slow feedbacks, especially change of ice sheet size and atmospheric CO2, amplify the total Earth system sensitivity by an amount that depends on the time scale considered. Ice sheet response time is poorly defined, but we show that the slow response and hysteresis in prevailing ice sheet models are exaggerated. We use a global model, simplified to essential processes, to investigate state dependence of climate sensitivity, finding an increased sensitivity towards warmer climates, as low cloud cover is diminished and increased water vapour elevates the tropopause. Burning all fossil fuels, we conclude, would make most of the planet uninhabitable by humans, thus calling into question strategies that emphasize adaptation to climate change.

Global Deliberative Democracy and Climate Change: Insights from World Wide Views on Global Warming in Australia

Directory of Open Access Journals (Sweden)

Chris Riedy

2011-12-01

Full Text Available On 26 September 2009, approximately 4,000 citizens in 38 countries participated in World Wide Views on Global Warming (WWViews. WWViews was an ambitious first attempt to convene a deliberative mini-public at a global scale, giving people from around the world an opportunity to deliberate on international climate policy and to make recommendations to the decision-makers meeting at the United Nations Climate Change Conference in Copenhagen (COP-15 in December 2009. In this paper, we examine the role that deliberative mini-publics can play in facilitating the emergence of a global deliberative system for climate change response. We pursue this intent through a reflective evaluation of the Australian component of the World Wide Views on Global Warming project (WWViews. Our evaluation of WWViews is mixed. The Australian event was delivered with integrity and feedback from Australian participants was almost universally positive. Globally, WWViews demonstrated that it is feasible to convene a global mini-public to deliberate on issues of global relevance, such as climate change. On the other hand, the contribution of WWViews towards the emergence of a global deliberative system for climate change response was limited and it achieved little influence on global climate change policy. We identify lessons for future global mini-publics, including the need to prioritise the quality of deliberation and provide flexibility to respond to cultural and political contexts in different parts of the world. Future global mini-publics may be more influential if they seek to represent discourse diversity in addition to demographic profiles, use designs that maximise the potential for transmission from public to empowered space, run over longer time periods to build momentum for change and experiment with ways of bringing global citizens together in a single process instead of discrete national events.

Molecular mechanism of Endosulfan action in mammals

Indian Academy of Sciences (India)

Keywords. DNA damage; double-strand break; genomic instability; infertility; MMEJ; NHEJ; pesticides. Abstract. Endosulfan is a broad-spectrum organochlorine pesticide, speculated to be detrimental to human health in areas ofactive exposure. However, the molecular insights to its mechanism of action remain poorly ...

Carbon dioxide-dependent regulation of NF-κB family members RelB and p100 gives molecular insight into CO2-dependent immune regulation.

Science.gov (United States)

Keogh, Ciara E; Scholz, Carsten C; Rodriguez, Javier; Selfridge, Andrew C; von Kriegsheim, Alexander; Cummins, Eoin P

2017-07-07

CO 2 is a physiological gas normally produced in the body during aerobic respiration. Hypercapnia (elevated blood pCO 2 >≈50 mm Hg) is a feature of several lung pathologies, e.g. chronic obstructive pulmonary disease. Hypercapnia is associated with increased susceptibility to bacterial infections and suppression of inflammatory signaling. The NF-κB pathway has been implicated in these effects; however, the molecular mechanisms underpinning cellular sensitivity of the NF-κB pathway to CO 2 are not fully elucidated. Here, we identify several novel CO 2 -dependent changes in the NF-κB pathway. NF-κB family members p100 and RelB translocate to the nucleus in response to CO 2 A cohort of RelB protein-protein interactions ( e.g. with Raf-1 and IκBα) are altered by CO 2 exposure, although others are maintained ( e.g. with p100). RelB is processed by CO 2 in a manner dependent on a key C-terminal domain located in its transactivation domain. Loss of the RelB transactivation domain alters NF-κB-dependent transcriptional activity, and loss of p100 alters sensitivity of RelB to CO 2 Thus, we provide molecular insight into the CO 2 sensitivity of the NF-κB pathway and implicate altered RelB/p100-dependent signaling in the CO 2 -dependent regulation of inflammatory signaling. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Thermodynamics and kinetics of molecular motors.

Science.gov (United States)

Astumian, R Dean

2010-06-02

Molecular motors are first and foremost molecules, governed by the laws of chemistry rather than of mechanics. The dynamical behavior of motors based on chemical principles can be described as a random walk on a network of states. A key insight is that any molecular motor in solution explores all possible motions and configurations at thermodynamic equilibrium. By using input energy and chemical design to prevent motion that is not wanted, what is left behind is the motion that is desired. This review is focused on two-headed motors such as kinesin and Myosin V that move on a polymeric track. By use of microscopic reversibility, it is shown that the ratio between the number of forward steps and the number of backward steps in any sufficiently long time period does not directly depend on the mechanical properties of the linker between the two heads. Instead, this ratio is governed by the relative chemical specificity of the heads in the front-versus-rear position for the fuel, adenosine triphosphate and its products, adenosine diphosphate and inorganic phosphate. These insights have been key factors in the design of biologically inspired synthetic molecular walkers constructed out of DNA or out of small organic molecules. Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Biotic and Biogeochemical Feedbacks to Climate Change

Science.gov (United States)

Torn, M. S.; Harte, J.

2002-12-01

, observational, or modeling, can adequately capture the complex factors that govern species distributions over relevant spatial and temporal scales, careful integration of these methods can yield needed insights. The potential for large, rapid, or unexpected feedbacks of biogeochemistry and energy balance to climate change make this a worthwhile challenge.

Perceptions of climate-related risk among water sector professionals in Africa-Insights from the 2016 African Water Association Congress.

Science.gov (United States)

Connolly, Katherine; Mbutu, Mwaura; Bartram, Jamie; Fuente, David

2018-04-23

The ability of water and wastewater utilities to provide safe and reliable water and sanitation services now and in the future will be determined, in part, by their resilience to climate change. Investment in infrastructure, planning, and operational practices that increase resilience are affected, in turn, by how water sector professionals perceive the risks posed to utilities by climate change and its related impacts. We surveyed water sector professionals at the 2016 African Water Association's Congress in Nairobi, Kenya to assess their perceptions of climate-specific and general risks that may disrupt utility service. We find that water sector professionals are most concerned about climate-specific and general risks that affect utility water supplies (quantity), followed by adequacy of utility infrastructure. We also find that professionals tend to rank climate-specific risks as less concerning than general risks facing utilities. Furthermore, non-utility professionals are more concerned about climate-specific risks and climate change in general than utility professionals. These findings highlight the multiple, competing risks utilities face and the need for adaptation strategies that simultaneously address climate-specific and general concerns of utilities. Copyright © 2018 Elsevier GmbH. All rights reserved.

Climate change trends and environmental impacts in the Makonde Communal Lands, Zimbabwe

Directory of Open Access Journals (Sweden)

Ishumael Sango

2015-07-01

Full Text Available During the last century, climate has increasingly become variable and changeable, with significant deviations from the observed normal averages, which often leads to disruptive consequences to ecosystems and livelihoods. Climate change induced environmental challenges are viewed to be particularly severe to economically challenged tropical societies including the Zimbabwean rural communities. We sought to determine local level climate change trends and associated biophysical implications in the Makonde Communal Lands of Zimbabwe. Our findings suggest that there has been significant climate change in the Makonde Communal Lands since 1962. The climate change observed has induced the deterioration of ecosystem productivity, diversity and services, to the detriment of human livelihoods. We provide insights into how to better understand local level dynamics between climate change and local ecosystem goods and services as the basis of livelihood in marginalised rural communities. Among the key reasons for concern about impacts of anthropogenic activities on climate is the fact that changing climate has direct impacts on the biophysical world, which in turn is a vital asset for human livelihoods, economies and general well-being.

New insight in the microscopic mechanism of the catalytic synthesis of ammonia

DEFF Research Database (Denmark)

Hansen, Flemming Yssing; Henriksen, Niels Engholm; Billing, Gert D.

1995-01-01

Theoretical quantum calculations and molecular beam experiments of the dissociative chemisorption of N-2 molecules on catalytic active metal surfaces have given new insight in the fundamental process of the ammonia synthesis. This new approach to the study of catalytic process supplements the con...

Interannual to multidecadal climate forcings on groundwater resources of the U.S. West Coast

Science.gov (United States)

Velasco, Elzie M.; Gurdak, Jason J.; Dickinson, Jesse; Ferré, T.P.A.; Corona, Claudia

2017-01-01

Study regionThe U.S. West Coast, including the Pacific Northwest and California Coastal Basins aquifer systems.Study focusGroundwater response to interannual to multidecadal climate variability has important implications for security within the water–energy–food nexus. Here we use Singular Spectrum Analysis to quantify the teleconnections between AMO, PDO, ENSO, and PNA and precipitation and groundwater level fluctuations. The computer program DAMP was used to provide insight on the influence of soil texture, depth to water, and mean and period of a surface infiltration flux on the damping of climate signals in the vadose zone.New hydrological insights for the regionWe find that PDO, ENSO, and PNA have significant influence on precipitation and groundwater fluctuations across a north-south gradient of the West Coast, but the lower frequency climate modes (PDO) have a greater influence on hydrologic patterns than higher frequency climate modes (ENSO and PNA). Low frequency signals tend to be preserved better in groundwater fluctuations than high frequency signals, which is a function of the degree of damping of surface variable fluxes related to soil texture, depth to water, mean and period of the infiltration flux. The teleconnection patterns that exist in surface hydrologic processes are not necessarily the same as those preserved in subsurface processes, which are affected by damping of some climate variability signals within infiltrating water.

Molecular dynamics insight to phase transition in n-alkanes with carbon nanofillers

Directory of Open Access Journals (Sweden)

Monisha Rastogi

2015-05-01

Full Text Available The present work aims to investigate the phase transition, dispersion and diffusion behavior of nanocomposites of carbon nanotube (CNT and straight chain alkanes. These materials are potential candidates for organic phase change materials(PCMs and have attracted flurry of research recently. Accurate experimental evaluation of the mass, thermal and transport properties of such composites is both difficult as well as economically taxing. Additionally it is crucial to understand the factors that results in modification or enhancement of their characteristic at atomic or molecular level. Classical molecular dynamics approach has been extended to elucidate the same. Bulk atomistic models have been generated and subjected to rigorous multistage equilibration. To reaffirm the approach, both canonical and constant-temperature, constant- pressure ensembles were employed to simulate the models under consideration. Explicit determination of kinetic, potential, non-bond and total energy assisted in understanding the enhanced thermal and transport property of the nanocomposites from molecular point of view. Crucial parameters including mean square displacement and simulated self diffusion coefficient precisely define the balance of the thermodynamic and hydrodynamic interactions. Radial distribution function also reflected the density variation, strength and mobility of the nanocomposites. It is expected that CNT functionalization could improve the dispersion within n-alkane matrix. This would further ameliorate the mass and thermal properties of the composite. Additionally, the determined density was in good agreement with experimental data. Thus, molecular dynamics can be utilized as a high throughput technique for theoretical investigation of nanocomposites PCMs.

Molecular dynamics insight to phase transition in n-alkanes with carbon nanofillers

Energy Technology Data Exchange (ETDEWEB)

Rastogi, Monisha [School of Engineering, Indian Institute of Technology Mandi, Himachal Pradesh 175 001 (India); Vaish, Rahul, E-mail: rahul@iitmandi.ac.in [School of Engineering, Indian Institute of Technology Mandi, Himachal Pradesh 175 001 (India); Materials Research Centre, Indian Institute of Science, Bangalore 560 012 (India)

2015-05-15

The present work aims to investigate the phase transition, dispersion and diffusion behavior of nanocomposites of carbon nanotube (CNT) and straight chain alkanes. These materials are potential candidates for organic phase change materials(PCMs) and have attracted flurry of research recently. Accurate experimental evaluation of the mass, thermal and transport properties of such composites is both difficult as well as economically taxing. Additionally it is crucial to understand the factors that results in modification or enhancement of their characteristic at atomic or molecular level. Classical molecular dynamics approach has been extended to elucidate the same. Bulk atomistic models have been generated and subjected to rigorous multistage equilibration. To reaffirm the approach, both canonical and constant-temperature, constant- pressure ensembles were employed to simulate the models under consideration. Explicit determination of kinetic, potential, non-bond and total energy assisted in understanding the enhanced thermal and transport property of the nanocomposites from molecular point of view. Crucial parameters including mean square displacement and simulated self diffusion coefficient precisely define the balance of the thermodynamic and hydrodynamic interactions. Radial distribution function also reflected the density variation, strength and mobility of the nanocomposites. It is expected that CNT functionalization could improve the dispersion within n-alkane matrix. This would further ameliorate the mass and thermal properties of the composite. Additionally, the determined density was in good agreement with experimental data. Thus, molecular dynamics can be utilized as a high throughput technique for theoretical investigation of nanocomposites PCMs.

Teaching About Climate Change in Medical Education: An Opportunity.

Science.gov (United States)

Maxwell, Janie; Blashki, Grant

2016-04-26

Climate change threatens many of the gains in development and health over the last century. However, it could also be a catalyst for a necessary societal transformation to a sustainable and healthy future. Doctors have a crucial role in climate change mitigation and health system adaptation to prepare for emergent health threats and a carbon-constrained future. This paper argues that climate change should be integrated into medical education for three reasons: first, to prepare students for clinical practice in a climate-changing world; secondly, to promote public health and eco-health literacy; and finally, to deepen existing learning and strengthen graduate attributes. This paper builds on existing literature and the authors' experience to outline potential learning objectives, teaching methods and assessment tasks. In the wake of recent progress at the United Nations climate change conference, COP-21, it is hoped that this paper will assist universities to integrate teaching about climate change into medical education. Significance for public healthThere is a strong case for teaching about climate change in medical education. Anthropogenic climate change is accepted by scientists, governments and health authorities internationally. Given the dire implications for human health, climate change is of fundamental relevance to future doctors. Integrating climate change into medical education offers an opportunity for future doctors to develop skills and insights essential for clinical practice and a public health role in a climate-changing world. This echoes a broader call for improved public health literacy among medical graduates. This paper provides medical schools with a rationale and an outline for teaching on climate change.

Next steps towards a Climate Innovation Centre in Ghana. Discussion Paper

International Nuclear Information System (INIS)

De Coninck, H.C.; Wuertenberger, L.; Akon-Yamga, G.

2011-03-01

A climate innovation centre is an institution aimed at enabling development through catalysing climate technology research, development, market creation and policy. This discussion paper discusses the possibilities, considerations and next steps for a Climate Innovation Centre (CIC) in Ghana based on new insights within the government of Ghana, a mapping of climate innovation in Ghana, new developments in the international climate negotiations and other multilateral processes, and analysis conducted by ECN. The conclusion is that a Climate Innovation Centre can fill significant gaps in climate resilient development in Ghana. It seems important that a Ghana CIC does not become yet another institution that focuses on basic research in the area of climate change. Rather, it should connect the dots of the Ghanaian climate innovation system and act as a knowledge facilitator, also for the private sector. Various models of climate innovation centres exist. Before choices are made for a model or a combination of models for Ghana, it is recommended to allow for a broad stakeholder process. The World Bank's infoDev programme could be a model for such a process. Such stakeholder engagement should be aligned with other policymaking processes on adaptation and low-carbon development, as well as related policy arenas, such as agriculture, transport, waste and energy.

Next steps towards a Climate Innovation Centre in Ghana. Discussion Paper

Energy Technology Data Exchange (ETDEWEB)

De Coninck, H.C.; Wuertenberger, L. [ECN Policy Studies, Petten (Netherlands); Akon-Yamga, G. [Science and Technology Policy Research Institute STEPRI, Accra (Guinea)

2011-03-15

A climate innovation centre is an institution aimed at enabling development through catalysing climate technology research, development, market creation and policy. This discussion paper discusses the possibilities, considerations and next steps for a Climate Innovation Centre (CIC) in Ghana based on new insights within the government of Ghana, a mapping of climate innovation in Ghana, new developments in the international climate negotiations and other multilateral processes, and analysis conducted by ECN. The conclusion is that a Climate Innovation Centre can fill significant gaps in climate resilient development in Ghana. It seems important that a Ghana CIC does not become yet another institution that focuses on basic research in the area of climate change. Rather, it should connect the dots of the Ghanaian climate innovation system and act as a knowledge facilitator, also for the private sector. Various models of climate innovation centres exist. Before choices are made for a model or a combination of models for Ghana, it is recommended to allow for a broad stakeholder process. The World Bank's infoDev programme could be a model for such a process. Such stakeholder engagement should be aligned with other policymaking processes on adaptation and low-carbon development, as well as related policy arenas, such as agriculture, transport, waste and energy.

Structural insight into RNA recognition motifs: versatile molecular Lego building blocks for biological systems.

Science.gov (United States)

Muto, Yutaka; Yokoyama, Shigeyuki

2012-01-01

'RNA recognition motifs (RRMs)' are common domain-folds composed of 80-90 amino-acid residues in eukaryotes, and have been identified in many cellular proteins. At first they were known as RNA binding domains. Through discoveries over the past 20 years, however, the RRMs have been shown to exhibit versatile molecular recognition activities and to behave as molecular Lego building blocks to construct biological systems. Novel RNA/protein recognition modes by RRMs are being identified, and more information about the molecular recognition by RRMs is becoming available. These RNA/protein recognition modes are strongly correlated with their biological significance. In this review, we would like to survey the recent progress on these versatile molecular recognition modules. Copyright © 2012 John Wiley & Sons, Ltd.

Norwegian climate research. An evaluation

Energy Technology Data Exchange (ETDEWEB)

NONE

2012-06-15

scales with reduced uncertainty and increased spatial detail. Advances will provide climate information for national and international decision making. The focus is on physical, chemical and biological processes in the atmospheric, oceanic, terrestrial, and cryospheric systems that are relevant to the climate system. 2. The impacts of, and adaptation to, climate change and variability: insights into the impacts of climate change and variability on the natural environment and on society; i.e. research on how species and ecosystems will be affected and on how society will be affected through changes in food production, water availability, health, etc. 3. Institutions and instruments for response to climate change: research on national and international climate policy, institutions (norms, principles, organisations, strategies, measures and instruments) for reducing greenhouse gases and adapting to climate change. Analysis of how societal relations at multiple levels of governance need to change in order to deal with climate change. This includes issues related to economic growth and poverty reduction, migration, changes in the attitudes and behaviour of the population etc.(auth)

Impact of biogenic emissions on feedbacks in the climate system

Science.gov (United States)

Krüger, Olaf

2017-04-01

Impact of biogenic emissions on feedbacks in the climate system Bio-geophysical feedback between marine or continental ecosystems and the atmosphere potentially can alter climate change. A prominent feedback loop which is under discussion since 1983 bases on the emission of biologically produced gases - molecular oxygen, sulphur containing compounds and possibly isoprene, supersaturated in oceanic waters - into the marine troposphere. These by-products of phytoplankton metabolism lead to aerosol production and procure sustained influence on climate via modulation of cloud optical properties. In this contribution some findings related to the above mentioned climate processes are presented with special emphasis on marine ecosystems. A comparison of marine and continental ecosystems is made and different processes with major impact on feedbacks in the climate system are discussed.

Climate change impacts on food system

Science.gov (United States)

Zhang, X.; Cai, X.; Zhu, T.

2014-12-01

Food system includes biophysical factors (climate, land and water), human environments (production technologies and food consumption, distribution and marketing), as well as the dynamic interactions within them. Climate change affects agriculture and food systems in various ways. Agricultural production can be influenced directly by climatic factors such as mean temperature rising, change in rainfall patterns, and more frequent extreme events. Eventually, climate change could cause shift of arable land, alteration of water availability, abnormal fluctuation of food prices, and increase of people at risk of malnutrition. This work aims to evaluate how climate change would affect agricultural production biophysically and how these effects would propagate to social factors at the global level. In order to model the complex interactions between the natural and social components, a Global Optimization model of Agricultural Land and Water resources (GOALW) is applied to the analysis. GOALW includes various demands of human society (food, feed, other), explicit production module, and irrigation water availability constraint. The objective of GOALW is to maximize global social welfare (consumers' surplus and producers' surplus).Crop-wise irrigation water use in different regions around the world are determined by the model; marginal value of water (MVW) can be obtained from the model, which implies how much additional welfare benefit could be gained with one unit increase in local water availability. Using GOALW, we will analyze two questions in this presentation: 1) how climate change will alter irrigation requirements and how the social system would buffer that by price/demand adjustment; 2) how will the MVW be affected by climate change and what are the controlling factors. These results facilitate meaningful insights for investment and adaptation strategies in sustaining world's food security under climate change.

Climate conscious citizenship in a digital urban setting

DEFF Research Database (Denmark)

Andersen, Sophie Esmann; Nielsen, Anne Ellerup

2011-01-01

Climate change has challenged urban life, and as an omnipresent force, Nature sets the agenda for urban living. Using stakeholder theory to conceptualise urban life, we approach Nature as both an omnipresent stakeholder and an issue to be continuously addressed and related to. Adapting the stakeh......Climate change has challenged urban life, and as an omnipresent force, Nature sets the agenda for urban living. Using stakeholder theory to conceptualise urban life, we approach Nature as both an omnipresent stakeholder and an issue to be continuously addressed and related to. Adapting...... explore how the citizen is framed and invited to enact his/her responsibilities to the natural environment in an urban setting and how the digital mediation facilitates various forms of relations and climate conscious positions, incorporating both narcissistic desires, universal anxiety, moral obligations......, ethical virtue and image performance. Statements from the actual confessors/committers exemplify this. Thus, the paper provides insight into understanding the complexity of climate-conscious citizenship as a complex configuration of paradoxical, co-existing ethics and arguments....

Communicating climate change and health in the media.

Science.gov (United States)

Depoux, Anneliese; Hémono, Mathieu; Puig-Malet, Sophie; Pédron, Romain; Flahault, Antoine

2017-01-01

The translation of science from research to real-world change is a central goal of public health. Communication has an essential role to play in provoking a response to climate change. It must first raise awareness, make people feel involved and ultimately motivate them to take action. The goal of this research is to understand how the information related to this issue is being addressed and disseminated to different audiences-public citizens, politicians and key climate change stakeholders. Initial results show that the scientific voice struggles to globally highlight this issue to a general audience and that messages that address the topic do not meet the challenges, going from a dramatic framing to a basic adaptation framing. Communication experts can help inform scientists and policy makers on how to best share information about climate change in an engaging and motivating way. This study gives an insight about the key role of the media and communications in addressing themes relating to climate change and transmitting information to the public in order to take action.

Planning for climate change in small islands: insights from national hurricane preparedness in the Cayman Islands

International Nuclear Information System (INIS)

Tompkins, E.L.

2005-01-01

This paper examines contemporary national scale responses to tropical storm risk in a small island in the Caribbean to derive lessons for adapting to climate change. There is little empirical evidence to guide national planners on how to adapt to climate change, and less still on how to build on past adaptation experiences. The paper investigates the construction of institutional resilience and the process of adaptation to tropical storm risk by the Cayman Islands' Government from 1988 to 2002. It explains the roles of persuasion, exposure and collective action as key components in developing the ability to buffer external disturbance using models of institutional economics and social resilience concepts. The study finds that self-efficacy, strong local and international support networks, combined with a willingness to act collectively and to learn from mistakes appear to have increased the resilience of the Cayman Islands' Government to tropical storm risk. The lessons learned from building resilience to storm risk can contribute to the creation of national level adaptive capacity to climate change, but climate change has to be prioritised before these lessons can be transferred. (author)

Planning for climate change in small islands: insights from national hurricane preparedness in the Cayman Islands

Energy Technology Data Exchange (ETDEWEB)

Tompkins, E.L. [University of East Anglia, Norwich (United Kingdom). School of Environmental Sciences

2005-07-01

This paper examines contemporary national scale responses to tropical storm risk in a small island in the Caribbean to derive lessons for adapting to climate change. There is little empirical evidence to guide national planners on how to adapt to climate change, and less still on how to build on past adaptation experiences. The paper investigates the construction of institutional resilience and the process of adaptation to tropical storm risk by the Cayman Islands' Government from 1988 to 2002. It explains the roles of persuasion, exposure and collective action as key components in developing the ability to buffer external disturbance using models of institutional economics and social resilience concepts. The study finds that self-efficacy, strong local and international support networks, combined with a willingness to act collectively and to learn from mistakes appear to have increased the resilience of the Cayman Islands' Government to tropical storm risk. The lessons learned from building resilience to storm risk can contribute to the creation of national level adaptive capacity to climate change, but climate change has to be prioritised before these lessons can be transferred. (author)

Early Eocene climatic optimum: Environmental impact on the North Iberian continental margin

NARCIS (Netherlands)

Payros, A.; Ortiz, S.; Millán, I.; Arostegi, J.; Orue-Etxebarria, X.; Apellaniz, E.

2015-01-01

The early Eocene climatic optimum, which constituted the peak of the long-term early Cenozoic global warming, had a significant impact on the environmental evolution of terrestrial and oceanic areas. Surprisingly, however, its influence on continental margins is poorly known. New insights are

Efficient and Flexible Climate Analysis with Python in a Cloud-Based Distributed Computing Framework

Science.gov (United States)

Gannon, C.

2017-12-01

As climate models become progressively more advanced, and spatial resolution further improved through various downscaling projects, climate projections at a local level are increasingly insightful and valuable. However, the raw size of climate datasets presents numerous hurdles for analysts wishing to develop customized climate risk metrics or perform site-specific statistical analysis. Four Twenty Seven, a climate risk consultancy, has implemented a Python-based distributed framework to analyze large climate datasets in the cloud. With the freedom afforded by efficiently processing these datasets, we are able to customize and continually develop new climate risk metrics using the most up-to-date data. Here we outline our process for using Python packages such as XArray and Dask to evaluate netCDF files in a distributed framework, StarCluster to operate in a cluster-computing environment, cloud computing services to access publicly hosted datasets, and how this setup is particularly valuable for generating climate change indicators and performing localized statistical analysis.

New insights into molecular diagnostic pathology of primary liver cancer: Advances and challenges.

Science.gov (United States)

Cong, Wen-Ming; Wu, Meng-Chao

2015-11-01

Primary liver cancer (PLC) is one of the most common malignancies worldwide with increasing incidence and accounts for the third leading cause of cancer-related mortality. Traditional morphopathology primarily emphasizes qualitative diagnosis of PLC, which is not sufficient to resolve the major concern of increasing the long-term treatment efficacy of PLC in clinical management for the modern era. Since the beginning of the 21st century, molecular pathology has played an active role in the investigation of the evaluation of the metastatic potential of PLC, detection of drug targets, prediction of recurrence risks, analysis of clonal origins, evaluation of the malignancy trend of precancerous lesions, and determination of clinical prognosis. As a result, many new progresses have been obtained, and new strategies of molecular-pathological diagnosis have been formed. Moreover, the new types of pathobiological diagnosis indicator systems for PLC have been preliminarily established. These achievements provide valuable molecular pathology-based guide for clinical formulation of individualized therapy programs for PLC. This review article briefly summarizes some relevant progresses of molecular-pathological diagnosis of PLC from the perspective of clinical translational application other than basic experimental studies. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Climate change and the UK house building sector: perceptions, impacts and adaptive capacity

International Nuclear Information System (INIS)

Hertin, J.; Berkhout, F.; Gann, D.; Barlow, J.

2003-01-01

This paper explores how climate change could affect the UK house-building sector, focusing on the question of how companies can adapt to changing climatic conditions. It presents the results of in-depth interviews in five house-building companies in the UK. We start from the assumption that climate change is only one driver among many, including technological innovation, shifting consumer expectations and changing regulation, that the industry faces. This approach draws on insights that are well established in the management and innovation literatures, but have often been neglected in studies of climate change. We report research about the perceptions of house builders about future impacts of climate change, potential adaptation measures that may be open to them and their ability to carry out these measures. The paper draws conclusions about the challenges that climate change presents to the UK house building industry. (author)

Implementation and new insights in molecular diagnostics for HIV infection.

Science.gov (United States)

Tsang, Hin-Fung; Chan, Lawrence Wing-Chi; Tong, Jennifer Chiu-Hung; Wong, Heong-Ting; Lai, Christopher Koon-Chi; Au, Thomas Chi-Chuen; Chan, Amanda Kit-Ching; Ng, Lawrence Po-Wah; Cho, William Chi-Shing; Wong, Sze-Chuen Cesar

2018-05-01

Acquired immunodeficiency syndrome (AIDS) is a kind of acquired disease that breaks down the immune system. Human immunodeficiency virus (HIV) is the causative agent of AIDS. By the end of 2016, there were 36.7 million people living with HIV worldwide. Early diagnosis can alert infected individuals to risk behaviors in order to control HIV transmission. Infected individuals are also benefited from proper treatment and management upon early diagnosis. Thanks to the public awareness of the disease, the annual increase of new HIV infections has been slowly declining over the past decades. The advent of molecular diagnostics has allowed early detection and better management of HIV infected patients. Areas covered: In this review, the authors summarized and discussed the current and future technologies in molecular diagnosis as well as the biomarkers developed for HIV infection. Expert Commentary: A simple and rapid detection of viral load is important for patients and doctors to monitor HIV progression and antiretroviral treatment efficiency. In the near future, it is expected that new technologies such as digital PCR and CRISPR-based technology will play more important role in HIV detection and patient management.

Nuclear molecular halo: the ubiquitous occurrence of van der Waals molecular states near threshold in molecular, nuclear and particle physics

International Nuclear Information System (INIS)

Gai, Moshe

1999-01-01

The observation of large E1 strength near threshold in the electromagnetic dissociation of 11 Li poses a fundamental question: Is the large E1 strength due to the threshold or is it due to a low lying E1 state? Such molecular cluster states were observed in 18 O and in several nuclei near the drip line. We discuss the nature of the threshold effect as well as review the situation in Molecular (and Particle Physics) where such Molecular States are observed near the dissociation limit. We suggest that the situation in 11 Li is reminiscent of the argon-benzene molecule where the argon atom is loosely bound by a polarization (van der Waals) mechanism and thus leads to a very extended object lying near the dissociation limit. Such states are also suggested to dominate the structure of mesons [α 0 (980), f 0 (975)] and baryons [λ(1405)] with proposed Kaon molecular structure (Dalitz) near threshold. The inspection of such states throughout Physics allows us to gain insight into this phenomenon and suggest that a new collective Molecular Dipole Degree of Freedom plays a major role in the structure of hadrons (halo nuclei, mesons and baryons), and that quantitative tools such as the E1 Molecular Sum Rule are useful for elucidating the nature of the observed low lying E1 strength in halo nuclei. (author)

Communicating Climate Change through ICT-Based Visualization: Towards an Analytical Framework

Directory of Open Access Journals (Sweden)

Björn-Ola Linnér

2013-11-01

Full Text Available The difficulties in communicating climate change science to the general public are often highlighted as one of the hurdles for support of enhanced climate action. The advances of interactive visualization using information and communication technology (ICT are claimed to be a game-changer in our ability to communicate complex issues. However, new analytical frameworks are warranted to analyse the role of such technologies. This paper develops a novel framework for analyzing the content, form, context and relevance of ICT-based visualization of climate change, based on insights from literature on climate change communication. Thereafter, we exemplify the analytical framework by applying it to a pilot case of ICT-based climate visualization in a GeoDome. Possibilities to use affordable advanced ICT-based visualization devices in science and policy communication are rapidly expanding. We thus see wider implications and applications of the analytical framework not only for other ICT environments but also other issue areas in sustainability communication.

Extreme Weather Events and Climate Change Attribution

Energy Technology Data Exchange (ETDEWEB)

Thomas, Katherine [National Academy of Sciences, Washington, DC (United States)

2016-03-31

A report from the National Academies of Sciences, Engineering, and Medicine concludes it is now possible to estimate the influence of climate change on some types of extreme events. The science of extreme event attribution has advanced rapidly in recent years, giving new insight to the ways that human-caused climate change can influence the magnitude or frequency of some extreme weather events. This report examines the current state of science of extreme weather attribution, and identifies ways to move the science forward to improve attribution capabilities. Confidence is strongest in attributing types of extreme events that are influenced by climate change through a well-understood physical mechanism, such as, the more frequent heat waves that are closely connected to human-caused global temperature increases, the report finds. Confidence is lower for other types of events, such as hurricanes, whose relationship to climate change is more complex and less understood at present. For any extreme event, the results of attribution studies hinge on how questions about the event's causes are posed, and on the data, modeling approaches, and statistical tools chosen for the analysis.

Structural insights of Staphylococcus aureus FtsZ inhibitors through molecular docking, 3D-QSAR and molecular dynamics simulations.

Science.gov (United States)

Ballu, Srilata; Itteboina, Ramesh; Sivan, Sree Kanth; Manga, Vijjulatha

2018-02-01

Filamentous temperature-sensitive protein Z (FtsZ) is a protein encoded by the FtsZ gene that assembles into a Z-ring at the future site of the septum of bacterial cell division. Structurally, FtsZ is a homolog of eukaryotic tubulin but has low sequence similarity; this makes it possible to obtain FtsZ inhibitors without affecting the eukaryotic cell division. Computational studies were performed on a series of substituted 3-arylalkoxybenzamide derivatives reported as inhibitors of FtsZ activity in Staphylococcus aureus. Quantitative structure-activity relationship models (QSAR) models generated showed good statistical reliability, which is evident from r 2 ncv and r 2 loo values. The predictive ability of these models was determined and an acceptable predictive correlation (r 2 Pred ) values were obtained. Finally, we performed molecular dynamics simulations in order to examine the stability of protein-ligand interactions. This facilitated us to compare free binding energies of cocrystal ligand and newly designed molecule B1. The good concordance between the docking results and comparative molecular field analysis (CoMFA)/comparative molecular similarity indices analysis (CoMSIA) contour maps afforded obliging clues for the rational modification of molecules to design more potent FtsZ inhibitors.

Climate and demography in early prehistory: using calibrated (14)C dates as population proxies.

Science.gov (United States)

Riede, Felix

2009-04-01

Although difficult to estimate for prehistoric hunter-gatherer populations, demographic variables-population size, density, and the connectedness of demes-are critical for a better understanding of the processes of material culture change, especially in deep prehistory. Demography is the middle-range link between climatic changes and both biological and cultural evolutionary trajectories of human populations. Much of human material culture functions as a buffer against climatic changes, and the study of prehistoric population dynamics, estimated through changing frequencies of calibrated radiocarbon dates, therefore affords insights into how effectively such buffers operated and when they failed. In reviewing a number of case studies (Mesolithic Ireland, the origin of the Bromme culture, and the earliest late glacial human recolonization of southern Scandinavia), I suggest that a greater awareness of demographic processes, and in particular of demographic declines, provides many fresh insights into what structured the archaeological record. I argue that we cannot sideline climatic and environmental factors or extreme geophysical events in our reconstructions of prehistoric culture change. The implications of accepting demographic variability as a departure point for evaluating the archaeological record are discussed.

Climate change: The 2015 Paris Agreement thresholds and Mediterranean basin ecosystems.

Science.gov (United States)

Guiot, Joel; Cramer, Wolfgang

2016-10-28

The United Nations Framework Convention on Climate Change Paris Agreement of December 2015 aims to maintain the global average warming well below 2°C above the preindustrial level. In the Mediterranean basin, recent pollen-based reconstructions of climate and ecosystem variability over the past 10,000 years provide insights regarding the implications of warming thresholds for biodiversity and land-use potential. We compare scenarios of climate-driven future change in land ecosystems with reconstructed ecosystem dynamics during the past 10,000 years. Only a 1.5°C warming scenario permits ecosystems to remain within the Holocene variability. At or above 2°C of warming, climatic change will generate Mediterranean land ecosystem changes that are unmatched in the Holocene, a period characterized by recurring precipitation deficits rather than temperature anomalies. Copyright © 2016, American Association for the Advancement of Science.

Molecular-level Insight into the Spectral Tuning Mechanism of the DsRed Chromophore

DEFF Research Database (Denmark)

List, Nanna Holmgaard; Olsen, Jógvan Magnus Haugaard; Jensen, Hans Jørgen Aagaard

2012-01-01

the protein. Our results indicate that this mainly is attributable to counter-directional contributions stemming from Lys163 and the conserved Arg95 with the former additionally identified as a key residue in the color tuning mechanism. The results provide new insights into the tuning mechanism of Ds...

Influences of subtropical jet and Tibetan Plateau on precipitation pattern in Asia : Insights from regional climate modeling

NARCIS (Netherlands)

Sato, Tomonori

2009-01-01

Large topographic features, like the Tibetan Plateau (TP) and the Rocky Mountains, have significant impacts on Earth's climate. Numerical experiments were carried out using a regional climate model in order to study the sensitivity of rainfall distribution to the TP's thermal/dynamic effects and

Climate change, nuclear power, and the adaptation-mitigation dilemma

International Nuclear Information System (INIS)

Kopytko, Natalie; Perkins, John

2011-01-01

Many policy-makers view nuclear power as a mitigation for climate change. Efforts to mitigate and adapt to climate change, however, interact with existing and new nuclear power plants, and these installations must contend with dilemmas between adaptation and mitigation. This paper develops five criteria to assess the adaptation-mitigation dilemma on two major points: (1) the ability of nuclear power to adapt to climate change and (2) the potential for nuclear power operation to hinder climate change adaptation. Sea level rise models for nine coastal sites in the United States, a review of US Nuclear Regulatory Commission documents, and reports from France's nuclear regulatory agency provided insights into issues that have arisen from sea level rise, shoreline erosion, coastal storms, floods, and heat waves. Applying the criteria to inland and coastal nuclear power plants reveals several weaknesses. Safety stands out as the primary concern at coastal locations, while inland locations encounter greater problems with interrupted operation. Adapting nuclear power to climate change entails either increased expenses for construction and operation or incurs significant costs to the environment and public health and welfare. Mere absence of greenhouse gas emissions is not sufficient to assess nuclear power as a mitigation for climate change. - Research Highlights: → The adaptation-mitigation criteria reveal nuclear power's vulnerabilities. → Climate change adaptation could become too costly at many sites. → Nuclear power operation jeopardizes climate change adaptation. → Extreme climate events pose a safety challenge.

Microsecond atomic-scale molecular dynamics simulations of polyimides

NARCIS (Netherlands)

Lyulin, S.V.; Gurtovenko, A.A.; Larin, S.V.; Nazarychev, V.M.; Lyulin, A.V.

2013-01-01

We employ microsecond atomic-scale molecular dynamics simulations to get insight into the structural and thermal properties of heat-resistant bulk polyimides. As electrostatic interactions are essential for the polyimides considered, we propose a two-step equilibration protocol that includes long

A linear projection for the timing of unprecedented climate in Korea

Science.gov (United States)

Shin, Ho-Jeong; Jang, Chan Joo; Chung, Il-Ung

2017-11-01

Recently we have had abnormal weather events worldwide that are attributed by climate scientists to the global warming induced by human activities. If the global warming continues in the future and such events occur more frequently and someday become normal, we will have an unprecedented climate. This study intends to answer when we will have an unprecedented warm climate, focusing more on the regional characteristics of the timing of unprecedented climate. Using an in-situ observational data from weather stations of annual-mean surface air temperature in Korea from 1973 to 2015, we estimate a timing of unprecedented climate with a linear regression method. Based on the in-situ data with statistically significant warming trends at 95% confidence level, an unprecedented climate in Korea is projected to occur first in Cheongju by 2043 and last in Haenam by 2168. This 125-year gap in the timing indicates that a regional difference in timing of unprecedented climate is considerably large in Korea. Despite the high sensitivity of linear estimation to the data period and resolution, our findings on the large regional difference in timing of unprecedented climate can give an insight into making policies for climate change mitigation and adaptation, not only for the central government but for provincial governments.

Library of molecular associations: curating the complex molecular basis of liver diseases

Directory of Open Access Journals (Sweden)

Maass Thorsten

2010-03-01

Full Text Available Abstract Background Systems biology approaches offer novel insights into the development of chronic liver diseases. Current genomic databases supporting systems biology analyses are mostly based on microarray data. Although these data often cover genome wide expression, the validity of single microarray experiments remains questionable. However, for systems biology approaches addressing the interactions of molecular networks comprehensive but also highly validated data are necessary. Results We have therefore generated the first comprehensive database for published molecular associations in human liver diseases. It is based on PubMed published abstracts and aimed to close the gap between genome wide coverage of low validity from microarray data and individual highly validated data from PubMed. After an initial text mining process, the extracted abstracts were all manually validated to confirm content and potential genetic associations and may therefore be highly trusted. All data were stored in a publicly available database, Library of Molecular Associations http://www.medicalgenomics.org/databases/loma/news, currently holding approximately 1260 confirmed molecular associations for chronic liver diseases such as HCC, CCC, liver fibrosis, NASH/fatty liver disease, AIH, PBC, and PSC. We furthermore transformed these data into a powerful resource for molecular liver research by connecting them to multiple biomedical information resources. Conclusion Together, this database is the first available database providing a comprehensive view and analysis options for published molecular associations on multiple liver diseases.

Advances in molecular vibrations and collision dynamics molecular clusters

CERN Document Server

Bacic, Zatko

1998-01-01

This volume focuses on molecular clusters, bound by van der Waals interactions and hydrogen bonds. Twelve chapters review a wide range of recent theoretical and experimental advances in the areas of cluster vibrations, spectroscopy, and reaction dynamics. The authors are leading experts, who have made significant contributions to these topics.The first chapter describes exciting results and new insights in the solvent effects on the short-time photo fragmentation dynamics of small molecules, obtained by combining heteroclusters with femtosecond laser excitation. The second is on theoretical work on effects of single solvent (argon) atom on the photodissociation dynamics of the solute H2O molecule. The next two chapters cover experimental and theoretical aspects of the energetics and vibrations of small clusters. Chapter 5 describes diffusion quantum Monte Carlo calculations and non additive three-body potential terms in molecular clusters. The next six chapters deal with hydrogen-bonded clusters, refle...

Climate change, humidity, and mortality in the United States

Science.gov (United States)

Barreca, Alan I.

2014-01-01

This paper estimates the effects of humidity and temperature on mortality rates in the United States (c. 1973–2002) in order to provide an insight into the potential health impacts of climate change. I find that humidity, like temperature, is an important determinant of mortality. Coupled with Hadley CM3 climate-change predictions, I project that mortality rates are likely to change little on the aggregate for the United States. However, distributional impacts matter: mortality rates are likely to decline in cold and dry areas, but increase in hot and humid areas. Further, accounting for humidity has important implications for evaluating these distributional effects. PMID:25328254

Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068

KAUST Repository

Dineshram, R

2016-01-01

The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.

Prolactin and teleost ionocytes: new insights into cellular and molecular targets of prolactin in vertebrate epithelia

Science.gov (United States)

Breves, Jason P.; McCormick, Stephen D.; Karlstrom, Rolf O.

2014-01-01

The peptide hormone prolactin is a functionally versatile hormone produced by the vertebrate pituitary. Comparative studies over the last six decades have revealed that a conserved function for prolactin across vertebrates is the regulation of ion and water transport in a variety of tissues including those responsible for whole-organism ion homeostasis. In teleost fishes, prolactin was identified as the “freshwater-adapting hormone”, promoting ion-conserving and water-secreting processes by acting on the gill, kidney, gut and urinary bladder. In mammals, prolactin is known to regulate renal, intestinal, mammary and amniotic epithelia, with dysfunction linked to hypogonadism, infertility, and metabolic disorders. Until recently, our understanding of the cellular mechanisms of prolactin action in fishes has been hampered by a paucity of molecular tools to define and study ionocytes, specialized cells that control active ion transport across branchial and epidermal epithelia. Here we review work in teleost models indicating that prolactin regulates ion balance through action on ion transporters, tight-junction proteins, and water channels in ionocytes, and discuss recent advances in our understanding of ionocyte function in the genetically and embryonically accessible zebrafish (Danio rerio). Given the high degree of evolutionary conservation in endocrine and osmoregulatory systems, these studies in teleost models are contributing novel mechanistic insight into how prolactin participates in the development, function, and dysfunction of osmoregulatory systems across the vertebrate lineage.

Multi-omics analysis provides insight to the Ignicoccus hospitalis-Nanoarchaeum equitans association.

Science.gov (United States)

Rawle, Rachel A; Hamerly, Timothy; Tripet, Brian P; Giannone, Richard J; Wurch, Louie; Hettich, Robert L; Podar, Mircea; Copié, Valerie; Bothner, Brian

2017-09-01

Studies of interspecies interactions are inherently difficult due to the complex mechanisms which enable these relationships. A model system for studying interspecies interactions is the marine hyperthermophiles Ignicoccus hospitalis and Nanoarchaeum equitans. Recent independently-conducted 'omics' analyses have generated insights into the molecular factors modulating this association. However, significant questions remain about the nature of the interactions between these archaea. We jointly analyzed multiple levels of omics datasets obtained from published, independent transcriptomics, proteomics, and metabolomics analyses. DAVID identified functionally-related groups enriched when I. hospitalis is grown alone or in co-culture with N. equitans. Enriched molecular pathways were subsequently visualized using interaction maps generated using STRING. Key findings of our multi-level omics analysis indicated that I. hospitalis provides precursors to N. equitans for energy metabolism. Analysis indicated an overall reduction in diversity of metabolic precursors in the I. hospitalis-N. equitans co-culture, which has been connected to the differential use of ribosomal subunits and was previously unnoticed. We also identified differences in precursors linked to amino acid metabolism, NADH metabolism, and carbon fixation, providing new insights into the metabolic adaptions of I. hospitalis enabling the growth of N. equitans. This multi-omics analysis builds upon previously identified cellular patterns while offering new insights into mechanisms that enable the I. hospitalis-N. equitans association. Our study applies statistical and visualization techniques to a mixed-source omics dataset to yield a more global insight into a complex system, that was not readily discernable from separate omics studies. Copyright © 2017 Elsevier B.V. All rights reserved.

New insights into thermal growing conditions of Portuguese grapevine varieties under changing climates

Science.gov (United States)

Santos, João A.; Costa, Ricardo; Fraga, Helder

2018-03-01

New decision support tools for Portuguese viticulture are urging under a climate change context. In the present study, heat and chilling accumulation conditions of a collection of 44 grapevine cultivars currently grown in Portugal are assessed at very high spatial resolution ( 1 km) and for 1981-2015. Two bioclimatic indices that incorporate non-linear plant-temperature relationships are selected for this purpose: growing degree hours—GDH (February-October) and chilling portions—CP (October-February). The current thermal growing conditions of each variety are examined and three clusters of grapevine cultivars are identified based on their GDH medians, thus assembling varieties with close heat accumulation requirements and providing more physiologically consistent information when compared to previous studies, as non-linear plant-temperature relationships are herein taken into account. These new clusters are also a complement to previous bioclimatic zoning. Ensemble mean projections under two anthropogenic-driven scenarios (RCP4.5 and RCP8.5, 2041-2070), from four EURO-CORDEX simulations, reveal a widespread increase of GDH and decrease of CP, but with spatial heterogeneities. The spatial variability of these indices throughout Portugal is projected to decrease (strongest increases of GDH in the coolest regions of the northeast) and to increase (strongest decreases of CP in the warmest regions of the south and west), respectively. The typical heat accumulation conditions of each cluster are projected to gradually shift north-eastwards and to higher-elevation areas, whereas insufficient chilling may represent a new challenge in warmer future climates. An unprecedented level of detail for a large collection of grapevine varieties in Portugal is provided, thus promoting a better planning of climate change adaptation measures.

Towards a climate-proof Netherlands. Summary routeplanner

International Nuclear Information System (INIS)

Van Drunen, M.

2007-03-01

There is no doubt about it: the climate is changing and the effects are now tangible and predictable. Scientific research has shown that even if we make significant reductions in greenhouse gas emissions (mitigation), climate change cannot be prevented. Which is why we have to adapt to make the effects of the changing climate acceptable: the Netherlands must be made climate-proof. To stimulate climate-proofing, four ministries and the Climate changes Spatial Planning (CcSP), Living with Water (LmW) and Habiforum research programmes have established a National Programme on Adapting Spatial Planning to Climate Change (ARK). The core research questions examined by ARK are: What is the nature and scale of the observable and expected impacts of climate change for various themes and economic sectors?; What spatial issues do they raise?; How can we tackle these spatial issues?; What dilemmas (technical, administrative, economic, social) will we face when trying to resolve these issues? The Routeplanner is the scientific arm of ARK: the three research programmes, assisted by other research institutes, supply ARK with scientific information and insights on climate-proofing the spatial development of the Netherlands. This brochure summarises the outcome of phase 2 of Routeplanner, which took place in 2006. It consisted of a Climate-proofing Baseline Assessment, a review (Quickscan) of knowledge gaps, formulation of adaptation strategies, a qualitative assessment of adaptation options, a quantitative assessment of adaptation options and identification of case studies. This brochure answers the following questions, as far as current scientific understanding permits: How will climate change affect the Netherlands?; What are the consequences of climate change for the Netherlands?; What must be done?; When must we act?; What examples are there of climate-proof strategies?; What next? The main conclusion of the Routeplanner is that climate change can cause considerable damage in

Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation

OpenAIRE

Joo, Seongjoon; Cho, In Jin; Seo, Hogyun; Son, Hyeoncheol Francis; Sagong, Hye-Young; Shin, Tae Joo; Choi, So Young; Lee, Sang Yup; Kim, Kyung-Jin

2018-01-01

Plastics, including poly(ethylene terephthalate) (PET), possess many desirable characteristics and thus are widely used in daily life. However, non-biodegradability, once thought to be an advantage offered by plastics, is causing major environmental problem. Recently, a PET-degrading bacterium, Ideonella sakaiensis, was identified and suggested for possible use in degradation and/or recycling of PET. However, the molecular mechanism of PET degradation is not known. Here we report the crystal ...

Mars Climate History: Insights From Impact Crater Wall Slope Statistics

Science.gov (United States)

Kreslavsky, Mikhail A.; Head, James W.

2018-02-01

We use the global distribution of the steepest slopes on crater walls derived from Mars Orbiter Laser Altimeter profile data to assess the magnitudes of degradational processes with latitude, altitude, and time. We independently confirm that Amazonian polar/high-latitude crater slope modification is substantial, but that craters in the low latitudes have essentially escaped significant slope modification since the Early Hesperian. We find that the total amount of crater wall degradation in the Late Noachian is very small in comparison to the circumpolar regions in the Late Amazonian, an observation that we interpret to mean that the Late Noachian climate was not characterized by persistent and continuous warm and wet conditions. A confirmed elevational zonality in degradation in the Early Hesperian is interpreted to mean that the atmosphere was denser than today.

Molecular Insights into the Genetic Diversity of Garcinia cambogia Germplasm Accessions

Directory of Open Access Journals (Sweden)

C Tharachand

2015-10-01

Full Text Available ABSTRACTIn this work, the genetic relationship among twelveGarcinia cambogia (Gaertn. Desr. accessions were evaluated using Random Amplified Polymorphic DNA markers. The samples were part of the germplasm collected and maintained at NBPGR Regional station, Thrissur, India. Out of thirty RAPD primers used for screening, seven primers produced a total of 128 polymorphic markers in twelve accessions. The Polymorphic Information Content (PIC ranged from 0.28 (OPA18 to 0.37 (OPA9 and Marker Index (MI ranged between 3.61 (OPA12 and 5.93 (OPA3 among the primers used. Jaccard's coefficient of genetic similarity ranged between 0.07 and 0.64. The dendrogram constructed based on the similarity matrix generated from the molecular and morphological data showed the genetic relationship among the sampled accessions. Mantel matrix test showed a positive correlation (r = 0.49 between the cluster analysis of RAPD data and morphological data. The clustering pattern in the molecular dendrogram and Principle Coordinate Analysis (PCoA showed that the genotypes were diverse, which was in congruence with the similarity index values and morphological dendrogram. High frequency of similarity values in the range of 0.11 to 0.17 suggested the existence of high genetic diversity among the accessions. The high level of genetic diversity among the studied accessions ofG.cambogia was also supported by the large variation in the morphological characters observed in the flowers, leaves, fruits and seeds of these sampled accessions. This is the first report for the molecular based genetic diversity studies for these accessions.

Insights into structural features of HDAC1 and its selectivity inhibition elucidated by Molecular dynamic simulation and Molecular Docking.

Science.gov (United States)

Sixto-López, Yudibeth; Bello, Martiniano; Correa-Basurto, José

2018-03-06

Histone deacetylases (HDACs) are a family of proteins whose main function is the removal of acetyl groups from lysine residues located on histone and non-histone substrates, which regulates gene transcription and other activities in cells. HDAC1 dysfunction has been implicated in cancer development and progression; thus, its inhibition has emerged as a new therapeutic strategy. Two additional metal binding sites (Site 1 and Site 2) in HDACs have been described that are primarily occupied by potassium ions, suggesting a possible structural role that affects HDAC activity. In this work, we explored the structural role of potassium ions in Site 1 and Site 2 and how they affect the interactions of compounds with high affinities for HDAC1 (AC1OCG0B, Chlamydocin, Dacinostat and Quisinostat) and SAHA (a pan-inhibitor) using molecular docking and molecular dynamics (MD) simulations in concert with a Molecular-Mechanics-Generalized-Born-Surface-Area (MMGBSA) approach. Four models were generated: one with a potassium ion (K + ) in both sites (HDAC1 k ), a second with K + only at site 1 (HDAC1 ks1 ), a third with K + only at site 2 (HDAC1 ks2 ) and a fourth with no K + (HDAC1 wk ). We found that the presence or absence of K + not only impacted the structural flexibility of HDAC1, but also its molecular recognition, consistent with experimental findings. These results could therefore be useful for further structure-based drug design studies addressing new HDAC1 inhibitors.

Country-Specific Effects of Climate Variability on Human Migration

Science.gov (United States)

Gray, Clark; Wise, Erika

2016-01-01

Involuntary human migration is among the social outcomes of greatest concern in the current era of global climate change. Responding to this concern, a growing number of studies have investigated the consequences of short to medium-term climate variability for human migration using demographic and econometric approaches. These studies have provided important insights, but at the same time have been significantly limited by lack of expertise in the use of climate data, access to cross-national data on migration, and attention to model specification. To address these limitations, we link data on internal and international migration over a 6-year period from 9,812 origin households in Kenya, Uganda, Nigeria, Burkina Faso and Senegal to high-resolution gridded climate data from both station and satellite sources. Analyses of these data using several plausible specifications reveal that climate variability has country-specific effects on migration: Migration tends to increase with temperature anomalies in Uganda, tends to decrease with temperature anomalies in Kenya and Burkina Faso, and shows no consistent relationship with temperature in Nigeria and Senegal. Consistent with previous studies, precipitation shows weak and inconsistent relationships with migration across countries. These results challenge generalizing narratives that foresee a consistent migratory response to climate change across the globe. PMID:27092012

Country-Specific Effects of Climate Variability on Human Migration.

Science.gov (United States)

Gray, Clark; Wise, Erika

2016-04-01

Involuntary human migration is among the social outcomes of greatest concern in the current era of global climate change. Responding to this concern, a growing number of studies have investigated the consequences of short to medium-term climate variability for human migration using demographic and econometric approaches. These studies have provided important insights, but at the same time have been significantly limited by lack of expertise in the use of climate data, access to cross-national data on migration, and attention to model specification. To address these limitations, we link data on internal and international migration over a 6-year period from 9,812 origin households in Kenya, Uganda, Nigeria, Burkina Faso and Senegal to high-resolution gridded climate data from both station and satellite sources. Analyses of these data using several plausible specifications reveal that climate variability has country-specific effects on migration: Migration tends to increase with temperature anomalies in Uganda, tends to decrease with temperature anomalies in Kenya and Burkina Faso, and shows no consistent relationship with temperature in Nigeria and Senegal. Consistent with previous studies, precipitation shows weak and inconsistent relationships with migration across countries. These results challenge generalizing narratives that foresee a consistent migratory response to climate change across the globe.

Methods for extracting climate indicator data from social media.

Science.gov (United States)

Fuka, M. Z.; Fuka, D. R.

2011-12-01

This paper shows how we've used the R software suite to extract climate indicator data from Twitter. In the course of this research we've collected extensive data sets of unsolicited observations ("tweets") for hundreds of climate-related phenological, biological, epidemiological and meteorological effects. R has proved itself in our work as a useful tool for manipulating those large data sets. Our experience from this effort has yielded a variety of insights on using R to extract geophysics-specific information from publicly accessible social media sources. We illustrate our methodology by mapping tweeted US armadillo sightings to explore the impact of climate variability on the extent of the animal's range. This example usefully demonstrates R's technical capabilities in collecting, time-stamping, geolocating, analyzing, visualizing and otherwise processing climate-related data derived from unsolicited social media postings. We also "mash-up" the data sets with those acquired by more traditional means, for example, temperature and precipitation data across the armadillo's US range. Our data-handling practice is extendable to social sharing services other than Twitter, providing the environmental modeling community an opportunity to access largely untapped resources of non-traditional climate indicator data to better understand the effects of climate change at local, regional and global scales.

Extended impacts of climate change on health and wellbeing

International Nuclear Information System (INIS)

Thomas, Felicity; Sabel, Clive E.; Morton, Katherine; Hiscock, Rosemary; Depledge, Michael H.

2014-01-01

Highlights: • Incorporates wellbeing into understandings of climate change impacts on health. • Considers a range of secondary impacts of climate change on health and wellbeing. • Examines co-benefits and dis-benefits of climate change adaptation and mitigation strategies for health and wellbeing. • Emphasises the spatially and socially differentiated repercussions of adaptation and mitigation measures. - Abstract: Anthropogenic climate change is progressively transforming the environment despite political and technological attempts to reduce greenhouse gas emissions to tackle global warming. Here we propose that greater insight and understanding of the health-related impacts of climate change can be gained by integrating the positivist approaches used in public health and epidemiology, with holistic social science perspectives on health in which the concept of ‘wellbeing’ is more explicitly recognised. Such an approach enables us to acknowledge and explore a wide range of more subtle, yet important health-related outcomes of climate change. At the same time, incorporating notions of wellbeing enables recognition of both the health co-benefits and dis-benefits of climate change adaptation and mitigation strategies across different population groups and geographical contexts. The paper recommends that future adaptation and mitigation policies seek to ensure that benefits are available for all since current evidence suggests that they are spatially and socially differentiated, and their accessibility is dependent on a range of contextually specific socio-cultural factors

Quantifying impacts of historical climate change in American River basin

Science.gov (United States)

Sultana, R.

2017-12-01

There is a near consensus among scientists that climate has been changing for the last few decades in different parts of the world. Some regions are already experiencing the impacts of these changes. Warmer climate can alter the hydrology and water resources around the globe. Historical data shows the temperature has been rising in California and affecting California's water resource by reducing snowfall and snowmelt runoff during spring season. In this study, Soil and Water Assessment Tool (SWAT) model is used to simulate the historical climate in American River basin, a mountainous watershed in California. The results show that warmer climate in the recent decades (1995-2014) have already have affected streamflow characteristics of the watershed. Compared to the 1965-1974, the mean annual streamflow has decreased more than 6% and the peak streamflow has shifted from May to April. Understanding the changes will assist the water resource managers with valuable insight on the effectiveness of mitigation strategies considered as of now.

Assessing confidence in management adaptation approaches for climate-sensitive ecosystems

International Nuclear Information System (INIS)

West, J M; Julius, S H; Weaver, C P

2012-01-01

A number of options are available for adapting ecosystem management to improve resilience in the face of climatic changes. However, uncertainty exists as to the effectiveness of these options. A report prepared for the US Climate Change Science Program reviewed adaptation options for a range of federally managed systems in the United States. The report included a qualitative uncertainty analysis of conceptual approaches to adaptation derived from the review. The approaches included reducing anthropogenic stressors, protecting key ecosystem features, maintaining representation, replicating, restoring, identifying refugia and relocating organisms. The results showed that the expert teams had the greatest scientific confidence in adaptation options that reduce anthropogenic stresses. Confidence in other approaches was lower because of gaps in understanding of ecosystem function, climate change impacts on ecosystems, and management effectiveness. This letter discusses insights gained from the confidence exercise and proposes strategies for improving future assessments of confidence for management adaptations to climate change. (letter)

First-principles insights on electron transport in V{sub 2}O{sub 5} nanostructures

Energy Technology Data Exchange (ETDEWEB)

Srivastava, Anurag [Advanced Materials Research Group, Computational Nanoscience and Technology Laboratory, Atal Bihari Vajpayee-Indian Institute of Information Technology and Management, Gwalior, Madhya Pradesh 474015 (India); Chandiramouli, R., E-mail: rcmoulii@gmail.com [School of Electrical and Electronics Engineering, Shanmugha Arts Science Technology and Research Academy (SASTRA) University, Tirumalaisamudram, Thanjavur, Tamil Nadu 613 401 (India)

2015-11-15

Graphical abstract: - Highlights: • Band structure and electron transport in V{sub 2}O{sub 5} nanostructure are investigated using density functional theory. • V{sub 2}O{sub 5} nanostructure exhibits semiconducting behavior. • The electron density is observed to be more in oxygen sites than in vanadium sites. • The electron transport in V{sub 2}O{sub 5} molecular device can be tuned with the applied bias voltage. - Abstract: The present report is on the electron transport properties of V{sub 2}O{sub 5} nanostructures, investigated using density functional theory. As the band structure of V{sub 2}O{sub 5} exhibits semiconducting nature, the V{sub 2}O{sub 5} nanostructures are designed as molecular device and the transport properties are studied. The density of electrons is found to be more in the oxygen sites than in vanadium sites. The device density of states shows that the density of electrons in the energy intervals depends on the applied bias voltage. The transmission spectrum gives the insight on the transport property of V{sub 2}O{sub 5} molecular device. The bias voltage drives the electrons across V{sub 2}O{sub 5} scattering region, where the transmission along V{sub 2}O{sub 5} molecular device mainly depends on the bias voltage. The findings of the present work give insights to fine-tune the transport property of V{sub 2}O{sub 5} molecular device upon varying the bias voltage.

Modeling European ruminant production systems: facing the challenges of climate change

DEFF Research Database (Denmark)

Kipling, Richard Philip; Bannink, Andre; Bellocchi, Gianni

2016-01-01

Ruminant production systems are important producers of food, support rural communities and culture, and help to maintain a range of ecosystem services including the sequestering of carbon in grassland soils. However, these systems also contribute significantly to climate change through greenhouse...... gas (GHG) emissions, while intensification of production has driven biodiversity and nutrient loss, and soil degradation. Modeling can offer insights into the complexity underlying the relationships between climate change, management and policy choices, food production, and the maintenance...... of ecosystem services. This paper 1) provides an overview of how ruminant systems modeling supports the efforts of stakeholders and policymakers to predict, mitigate and adapt to climate change and 2) provides ideas for enhancing modeling to fulfil this role. Many grassland models can predict plant growth...

Molecular-Scale Electronics: From Concept to Function.

Science.gov (United States)

Xiang, Dong; Wang, Xiaolong; Jia, Chuancheng; Lee, Takhee; Guo, Xuefeng

2016-04-13

Creating functional electrical circuits using individual or ensemble molecules, often termed as "molecular-scale electronics", not only meets the increasing technical demands of the miniaturization of traditional Si-based electronic devices, but also provides an ideal window of exploring the intrinsic properties of materials at the molecular level. This Review covers the major advances with the most general applicability and emphasizes new insights into the development of efficient platform methodologies for building reliable molecular electronic devices with desired functionalities through the combination of programmed bottom-up self-assembly and sophisticated top-down device fabrication. First, we summarize a number of different approaches of forming molecular-scale junctions and discuss various experimental techniques for examining these nanoscale circuits in details. We then give a full introduction of characterization techniques and theoretical simulations for molecular electronics. Third, we highlight the major contributions and new concepts of integrating molecular functionalities into electrical circuits. Finally, we provide a critical discussion of limitations and main challenges that still exist for the development of molecular electronics. These analyses should be valuable for deeply understanding charge transport through molecular junctions, the device fabrication process, and the roadmap for future practical molecular electronics.

Molecular characterization of PCN populations from Serbia

Directory of Open Access Journals (Sweden)

Oro Violeta

2012-01-01

Full Text Available The morphology of potato cyst nematodes (PCN was until recently almost the only way to identify these quarantine organisms. In the last two decades, molecular analyses contributed to faster and more efficient identification of two Globodera species (Globodera pallida and G. rostochiensis and allowed insight into the genetic structure of those parts that were practically inaccessible by morphological studies. Molecular characterization was performed in ITS1-5.8S-ITS2 region. The comparison was made with sequences of different foreign PCN populations via NCBI GenBank database. The results of molecular studies showed similarities and differences between local and foreign PCN populations in the part of genome that was studied.

Phylogeographic insights into cryptic glacial refugia.

Science.gov (United States)

Provan, Jim; Bennett, K D

2008-10-01

The glacial episodes of the Quaternary (2.6 million years ago-present) were a major factor in shaping the present-day distributions of extant flora and fauna, with expansions and contractions of the ice sheets rendering large areas uninhabitable for most species. Fossil records suggest that many species survived glacial maxima by retreating to refugia, usually at lower latitudes. Recently, phylogeographic studies have given support to the existence of previously unknown, or cryptic, refugia. Here we summarise many of these insights into the glacial histories of species in cryptic refugia gained through phylogeographic approaches. Understanding such refugia might be important as the Earth heads into another period of climate change, in terms of predicting the effects on species distribution and survival.

Advancements in the use of speleothems as climate archives

Science.gov (United States)

Wong, Corinne I.; Breecker, Daniel O.

2015-11-01

Speleothems have become a cornerstone of the approach to better understanding Earth's climatic teleconnections due to their precise absolute chronologies, their continuous or semicontinuous deposition and their global terrestrial distribution. We review the last decade of speleothem-related research, building off a similar review by McDermott (2004), in three themes - i) investigation of global teleconnections using speleothem-based climate reconstructions, ii) refinement of climate interpretations from speleothem proxies through cave monitoring, and iii) novel, technical methods of speleothem-based climate reconstructions. Speleothem records have enabled critical insight into the response of global hydroclimate to large climate changes. This includes the relevant forcings and sequence of climatic responses involved in glacial terminations and recognition of a global monsoon response to climate changes on orbital and millennial time scales. We review advancements in understanding of the processes that control speleothem δ13C values and introduce the idea of a direct atmospheric pCO2 influence. We discuss progress in understanding kinetic isotope fractionation, which, with further advances, may help quantify paleoclimate changes despite non-equilibrium formation of speleothems. This feeds into the potential of proxy system modeling to consider climatic, hydrological and biogeochemical processes with the objective of quantitatively interpreting speleothem proxies. Finally, we provide an overview of emerging speleothem proxies and novel approaches using existing proxies. Most recently, technical advancements made in the measurement of fluid inclusions are now yielding reliable determinations of paleotemperatures.

Molecular and genetic insights into an infantile epileptic encephalopathy – CDKL5 disorder

Science.gov (United States)

Zhou, Ailing; Han, Song

2017-01-01

Background The discovery that mutations in cyclin-dependent kinase-like 5 (CDKL5) gene are associated with infantile epileptic encephalopathy has stimulated world-wide research effort to understand the molecular and genetic basis of CDKL5 disorder. Given the large number of literature published thus far, this review aims to summarize current genetic studies, draw a consensus on proposed molecular functions, and point to gaps of knowledge in CDKL5 research. Methods A systematic review process was conducted using the PubMed search engine focusing on CDKL5 studies in the recent ten years. We analyzed these publications and summarized the findings into four sections: genetic studies, CDKL5 expression patterns, molecular functions, and animal models. We also discussed challenges and future directions in each section. Results On the clinical side, CDKL5 disorder is characterized by early onset epileptic seizures, intellectual disability, and stereotypical behaviors. On the research side, a series of molecular and genetic studies in human patients, cell cultures and animal models have established the causality of CDKL5 to the infantile epileptic encephalopathy, and pointed to a key role for CDKL5 in regulating neuronal function in the brain. Mouse models of CDKL5 disorder have also been developed, and notably, manifest behavioral phenotypes, mimicking numerous clinical symptoms of CDKL5 disorder and advancing CDKL5 research to the preclinical stage. Conclusions Given what we have learned thus far, future identification of robust, quantitative, and sensitive outcome measures would be the key in animal model studies, particularly in heterozygous females. In the meantime, molecular and cellular studies of CDKL5 should focus on mechanism-based investigation and aim to uncover druggable targets that offer the potential to rescue or ameliorate CDKL5 disorder-related phenotypes. PMID:28580010

Isotopic and molecular fractionation in combustion; three routes to molecular marker validation, including direct molecular 'dating' (GC/AMS)

Science.gov (United States)

Currie, L. A.; Klouda, G. A.; Benner, B. A.; Garrity, K.; Eglinton, T. I.

The identification of unique isotopic, elemental, and molecular markers for sources of combustion aerosol has growing practical importance because of the potential effects of fine particle aerosol on health, visibility and global climate. It is urgent, therefore, that substantial efforts be directed toward the validation of assumptions involving the use of such tracers for source apportionment. We describe here three independent routes toward carbonaceous aerosol molecular marker identification and validation: (1) tracer regression and multivariate statistical techniques applied to field measurements of mixed source, carbonaceous aerosols; (2) a new development in aerosol 14C metrology: direct, pure compound accelerator mass spectrometry (AMS) by off-line GC/AMS ('molecular dating'); and (3) direct observation of isotopic and molecular source emissions during controlled laboratory combustion of specific fuels. Findings from the combined studies include: independent support for benzo( ghi)perylene as a motor vehicle tracer from the first (statistical) and second (direct 'dating') studies; a new indication, from the third (controlled combustion) study, of a relation between 13C isotopic fractionation and PAH molecular fractionation, also linked with fuel and stage of combustion; and quantitative data showing the influence of both fuel type and combustion conditions on the yields of such species as elemental carbon and PAH, reinforcing the importance of exercising caution when applying presumed conservative elemental or organic tracers to fossil or biomass burning field data as in the first study.

Elements of a new climate agreement by 2015

Energy Technology Data Exchange (ETDEWEB)

Holm Olsen, K.; Fenhann, J.; Luetken, S.

2013-06-15

This year's Perspectives from UNEP and UNEP Risoe Centre in collaboration with the Global Green Growth Institute (GGGI) focuses on the elements of a new climate agreement by 2015 that will contribute to achieve the 2 deg. C limit for global warming. The first paper in this publication frames the global mitigation challenge based on the UNEP Emissions Gap Report 2012. The five other articles address key elements of a new climate agreement; emissions from international aviation, a vision for carbon markets up to 2020 and beyond, how green growth strategies can address the emissions gap, redesign of a REDD+ mechanism in response to the crisis of global deforestation and how NAMAs in Southern Africa can reconcile the gap between local and global objectives for development and climate change mitigation. The Perspectives series seeks to inspire policy- and decision makers by communicating the diverse insights and visions of leading actors in the arena of low carbon development in developing countries. (Author)

The Costs of Climate Change: A Study of Cholera in Tanzania

DEFF Research Database (Denmark)

Trærup, Sara Lærke Meltofte; Ortiz, Ramon A.; Markandya, Anil

2011-01-01

Increased temperatures and changes in rainfall patterns as a result of climate change are widely recognized to entail potentially serious consequences for human health, including an increased risk of diarrheal diseases. This study integrates historical data on temperature and rainfall...... to climate change are shown to be in the range of 0.32 to 1.4 percent of GDP in Tanzania 2030. The results provide useful insights into national-level estimates of the implications of climate change on the health sector and offer information which can feed into both national and international debates...... risk of cholera increases by 15 to 29 percent. Based on the modeling results, we project the number and costs of additional cases of cholera that can be attributed to climate change by 2030 in Tanzania for a 1 and 2 degree increase in temperatures, respectively. The total costs of cholera attributable...

Engaging informal audiences in learning about and responding to climate change through a portfolio of innovative approaches

Science.gov (United States)

Pfirman, S. L.; Brunacini, J.; Orlove, B. S.; Bachrach, E.; Hamilton, L.

2017-12-01

Informal learners have many different backgrounds, experiences, and perspectives. How can informal educators effectively reach such diverse audiences, meeting people where they are with regard to climate change? The Polar Learning and Responding: PoLAR Climate Change Education Partnership, supported by NSF, employs surveys, resource development, and research to develop innovative, evidence-based approaches that engage lifelong learners. General-public surveys on climate change yield insights on the knowledge and perceptions that informal learners bring to the table. That helps guide the creation of new tools for effective communication. For example, many people are unsure what causes sea level to rise. The Polar Explorer: Sea Level app uses a data and question-based approach guiding people through interactive maps to learn about melting land ice. In addition, people also tend to believe that climate impacts will happen in the future. Polar Voices podcasts feature Arctic Indigenous communities sharing first-hand experiences with climate change. Prior knowledge can be harnessed to enhance learning. Arctic SMARTIC engages people in role-playing negotiations with others to create a marine management plan. Climate game jams provide collaborative, creative spaces where participants learn as they interact with others. In each case participants, with all their knowledge and experience, are brought into group problem-solving. Understanding whom people trust for climate-change information offers insights that help them become climate communicators. Even those who are concerned about climate often do not discuss it with family and friends (Maibach et al. 2016), yet our research shows that family and friends are second only to scientists as trusted sources of climate information (Hamilton 2016). Fun and novel educational tools such as the EcoChains card game and the EcoKoin social networking app serve as conversation starters.

Structural insights into the recognition of phosphopeptide by the FHA domain of kanadaptin

DEFF Research Database (Denmark)

Xu, Qingping; Deller, Marc C; Nielsen, Tine K

2014-01-01

with a phosphopeptide mimic derived from a peptide segment from the N-terminus of a symmetry-related molecule as well as a sulfate bound to the structurally conserved phosphothreonine recognition cleft. This structure provides insights into the molecular recognition features utilized by this family of proteins...

Rethinking Social Contracts: Building Resilience in a Changing Climate

Directory of Open Access Journals (Sweden)

Karen O'Brien

2009-12-01

Full Text Available Social contracts play an important role in defining the reciprocal rights, obligations, and responsibilities between states and citizens. Climate change is creating new challenges for both states and citizens, inevitably forcing a rethinking of existing and evolving social contracts. In particular, the social arrangements that enhance the well-being and security of both present and future generations are likely to undergo dramatic transformations in response to ecosystem changes, more extreme weather events, and the consequences of social-ecological changes in distant locations. The types of social contracts that evolve in the face of a changing climate will have considerable implications for adaptation policies and processes. We consider how a resilience approach can contribute to new social contracts in the face of uncertainty and change. Examples from Norway, New Zealand, and Canada show how resilience thinking provides a new way of looking at social contracts, emphasizing the dynamics, links, and complexity of coupled social-ecological systems. Resilience thinking provides valuable insights on the characteristics of a new social contract, and social contract theory provides some insights on creating resilience and human security in a warming world.

Mechanistic and quantitative insight into cell surface targeted molecular imaging agent design.

Science.gov (United States)

Zhang, Liang; Bhatnagar, Sumit; Deschenes, Emily; Thurber, Greg M

2016-05-05

Molecular imaging agent design involves simultaneously optimizing multiple probe properties. While several desired characteristics are straightforward, including high affinity and low non-specific background signal, in practice there are quantitative trade-offs between these properties. These include plasma clearance, where fast clearance lowers background signal but can reduce target uptake, and binding, where high affinity compounds sometimes suffer from lower stability or increased non-specific interactions. Further complicating probe development, many of the optimal parameters vary depending on both target tissue and imaging agent properties, making empirical approaches or previous experience difficult to translate. Here, we focus on low molecular weight compounds targeting extracellular receptors, which have some of the highest contrast values for imaging agents. We use a mechanistic approach to provide a quantitative framework for weighing trade-offs between molecules. Our results show that specific target uptake is well-described by quantitative simulations for a variety of targeting agents, whereas non-specific background signal is more difficult to predict. Two in vitro experimental methods for estimating background signal in vivo are compared - non-specific cellular uptake and plasma protein binding. Together, these data provide a quantitative method to guide probe design and focus animal work for more cost-effective and time-efficient development of molecular imaging agents.

Raman spectroscopy as an advanced structural nanoprobe for conjugated molecular semiconductors

International Nuclear Information System (INIS)

Wood, Sebastian; Hollis, Joseph Razzell; Kim, Ji-Seon

2017-01-01

Raman spectroscopy has emerged as a powerful and important characterisation tool for probing molecular semiconducting materials. The useful optoelectronic properties of these materials arise from the delocalised π -electron density in the conjugated core of the molecule, which also results in large Raman scattering cross-sections and a strong coupling between its electronic states and vibrational modes. For this reason, Raman spectroscopy offers a unique insight into the properties of molecular semiconductors, including: chemical structure, molecular conformation, molecular orientation, and fundamental photo- and electro-chemical processes—all of which are critically important to the performance of a wide range of optical and electronic organic semiconductor devices. Experimentally, Raman spectroscopy is non-intrusive, non-destructive, and requires no special sample preparation, and so is suitable for a wide range of in situ measurements, which are particularly relevant to issues of thermal and photochemical stability. Here we review the development of the family of Raman spectroscopic techniques, which have been applied to the study of conjugated molecular semiconductors. We consider the suitability of each technique for particular circumstances, and the unique insights it can offer, with a particular focus on the significance of these measurements for the continuing development of stable, high performance organic electronic devices. (topical review)

Towards a climate-neutral water cycle; Op weg naar een klimaatneutrale waterketen

Energy Technology Data Exchange (ETDEWEB)

Frijns, J. [KWR Watercycle Research Institute, Nieuwegein (Netherlands); Mulder, M.; Roorda, J. [Grontmij, De Bilt (Netherlands)

2008-07-01

Insight is offered in the climate footprint of the domestic water cycle. Moreover options are provided with which the water sector can reduce its own negative impact on the climate. To realize this, the sectors drinking water, sewage and waste water can separately take a large number of (energy) measures [Dutch] Inzicht wordt gegeven in de klimaatvoetafdruk van de huishoudelijke waterketen. Tevens worden mogelijkheden aangedragen om als watersector de eigen negatieve impact op het klimaat te verkleinen. Daarvoor kunnen de sectoren drinkwater, riolering en afvalwater separaat een groot aantal (energie)maatregelen nemen.

Toward a Theoretical Framework for Studying Climate Change Policies: Insights from the Case Study of Singapore

OpenAIRE

Ai Sian Ng; May O. Lwin; Augustine Pang

2017-01-01

The world decided in December 2015 to take actions to reduce global warming. To contribute toward this goal, this research examines possible policy levers for inclusion in the climate change ratification plan. A case study of the measures taken by the Republic of Singapore, a low-lying 719.2 km2 island without natural resources in Asia, is conducted. Being vulnerable to climate change impact and yet having to balance her people’s needs and economic progress with limited resources, the measure...

Model confirmation in climate economics

Science.gov (United States)

Millner, Antony; McDermott, Thomas K. J.

2016-01-01

Benefit–cost integrated assessment models (BC-IAMs) inform climate policy debates by quantifying the trade-offs between alternative greenhouse gas abatement options. They achieve this by coupling simplified models of the climate system to models of the global economy and the costs and benefits of climate policy. Although these models have provided valuable qualitative insights into the sensitivity of policy trade-offs to different ethical and empirical assumptions, they are increasingly being used to inform the selection of policies in the real world. To the extent that BC-IAMs are used as inputs to policy selection, our confidence in their quantitative outputs must depend on the empirical validity of their modeling assumptions. We have a degree of confidence in climate models both because they have been tested on historical data in hindcasting experiments and because the physical principles they are based on have been empirically confirmed in closely related applications. By contrast, the economic components of BC-IAMs often rely on untestable scenarios, or on structural models that are comparatively untested on relevant time scales. Where possible, an approach to model confirmation similar to that used in climate science could help to build confidence in the economic components of BC-IAMs, or focus attention on which components might need refinement for policy applications. We illustrate the potential benefits of model confirmation exercises by performing a long-run hindcasting experiment with one of the leading BC-IAMs. We show that its model of long-run economic growth—one of its most important economic components—had questionable predictive power over the 20th century. PMID:27432964

Medulloblastoma: Molecular Genetics and Animal Models

Directory of Open Access Journals (Sweden)

Corey Raffel

2004-07-01

Full Text Available Medulloblastoma is a primary brain tumor found in the cerebellum of children. The tumor occurs in association with two inherited cancer syndromes: Turcot syndrome and Gorlin syndrome. Insights into the molecular biology of the tumor have come from looking at alterations in the genes altered in these syndromes, PTC and APC, respectively. Murine models of medulloblastoma have been constructed based on these alterations. Additional murine models that, while mimicking the appearance of the human tumor, seem unrelated to the human tumor's molecular alterations have been made. In this review, the clinical picture, origin, molecular biology, murine models of medulloblastoma are discussed. Although a great deal has been discovered about this tumor, the genetic alterations responsible for tumor development in a majority of patients have yet to be described.

Projections of future extreme weather losses under changes in climate and exposure

NARCIS (Netherlands)

Bouwer, L.M.

2013-01-01

Many attempts are made to assess future changes in extreme weather events due to anthropogenic climate change, but few studies have estimated the potential change in economic losses from such events. Projecting losses is more complex as it requires insight into the change in the weather hazard but

Insights on different participation schemes to meet climate goals

International Nuclear Information System (INIS)

Russ, Peter; Ierland, Tom van

2009-01-01

Models and scenarios to assess greenhouse gas mitigation action have become more diversified and detailed, allowing the simulation of more realistic global climate policy set-ups. In this paper, different participation schemes to meet different levels of radiative forcing are analysed. The focus is on scenarios that are in line with the 2 deg. C target. Typical stylised participation schemes are based either on a perfect global carbon market or delayed participation with targets only for developed countries, no actions by developing countries and no access to credits from offsetting mechanisms in developing countries. This paper adds an intermediate policy scenario assuming a gradual incorporation of all countries, including a gradually developing carbon market, and taking into account the ability to contribute of different parties. Perfect participation by all parties would be optimal, but it is shown that participation schemes involving a gradual and differentiated participation by all parties can substantially decrease global costs and still meet the 2 deg. C target. Carbon markets can compensate in part for those costs incurred by developing countries' own, autonomous mitigation actions that do not generate tradable emission credits.

Molecular discrimination and genetic relationships between some ...

African Journals Online (AJOL)

Cucurbita pepo ssp. pepo; zucchini group is a widely grown and economically important group belonging to genus Cucurbita, and being one of the easiest groups to cultivate in temperate climate with overwhelming production. Since, RAPD analysis provides a fast and reliable method for molecular characterization and ...

Mandate for the Nursing Profession to Address Climate Change Through Nursing Education.

Science.gov (United States)

Leffers, Jeanne; Levy, Ruth McDermott; Nicholas, Patrice K; Sweeney, Casey F

2017-11-01

The adverse health effects from climate change demand action from the nursing profession. This article examines the calls to action, the status of climate change in nursing education, and challenges and recommendations for nursing education related to climate change and human health. Discussion paper. The integration of climate change into nursing education is essential so that knowledge, skills, and insights critical for clinical practice in our climate-changing world are incorporated in curricula, practice, research, and policy. Our Ecological Planetary Health Model offers a framework for nursing to integrate relevant climate change education into nursing curricula and professional nursing education. Nursing education can offer a leadership role to address the mitigation, adaptation, and resilience strategies for climate change. An ecological framework is valuable for nursing education regarding climate change through its consideration of political, cultural, economic, and environmental interrelationships on human health and the health of the planet. Knowledge of climate change is important for integration into basic and advanced nursing education, as well as professional education for nurses to address adverse health impacts, climate change responses policy, and advocacy roles. For current and future nurses to provide care within a climate-changing environment, nursing education has a mandate to integrate knowledge about climate change issues across all levels of nursing education. Competence in nursing practice follows from knowledge and skill acquisition gained from integration of climate change content into nursing education. © 2017 Sigma Theta Tau International.

Environmental Forensics: Molecular Insight into Oil Spill Weathering Helps Advance High Magnetic Field FT-ICR Mass Spectrometry

Science.gov (United States)

McKenna, Amy

2013-03-01

events in the FT-ICR experiment. For example, the high density of peaks at each nominal mass unit provides unprecedented insight into how excitation conditions affect ion motion during detection. Aggregated oil (i.e., tar balls, tar mats) that reached the surface exhibits a more than two-fold increase in the total number of detected species, with an increased number of oxygenated species. Principal component analysis (PCA) applied to two possible source oils (contained within the same ship) and weathered samples provide the first application of FT-ICR MS for source identification. Molecular formulae from parent and weathered oil indicate that the lightest petroleum fractions (saturated hydrocarbons) are the most readily oxidized components, and can serve as a template to determine chemical transformations that occur throughout the water column. The ability to differentiate and catalogue compositional changes that occur to oil after its release into the environment relies heavily on gains achieved in nearly all steps in the FT-ICR mass spectral experiment required to accommodate larger ion populations inherent to heavily weathered crude oil. Here, we present the requirement for FT-ICR MS for comprehensive oil spill characterization, and highlight advances made to FT-ICR MS experimental conditions developed from petroleum characterization. Work supported by DMR-06-54118, NSF CHE-10-49753 (RAPID), BP/The Gulf of Mexico Research Initiative, and the State of Florida

Targeting molecular networks for drug research

Directory of Open Access Journals (Sweden)

José Pedro Pinto

2014-06-01

Full Text Available The study of molecular networks has recently moved into the limelight of biomedical research. While it has certainly provided us with plenty of new insights into cellular mechanisms, the challenge now is how to modify or even restructure these networks. This is especially true for human diseases, which can be regarded as manifestations of distorted states of molecular networks. Of the possible interventions for altering networks, the use of drugs is presently the most feasible. In this mini-review, we present and discuss some exemplary approaches of how analysis of molecular interaction networks can contribute to pharmacology (e.g., by identifying new drug targets or prediction of drug side effects, as well as listing pointers to relevant resources and software to guide future research. We also outline recent progress in the use of drugs for in vitro reprogramming of cells, which constitutes an example par excellence for altering molecular interaction networks with drugs.

Booklet for sub-national stakeholders heading towards the COP 21. Agricultural and forestry focus. Key concepts on the impacts of climate change, climate policies and economic tools: insight from French territories

International Nuclear Information System (INIS)

Depoues, Vivian; Leseur, Alexia; Bordier, Cecile; Foucherot, Claudine; Lacan, Lea; Planton, Serge; Blanchard, Michele; Lemonsu, Aude; Martin, Eric; Masson, Valery; Soubeyroux, Jean-Michel; Ouzeau, Gaelle; Dandin, Philippe; Pigeon, Gregoire; Duvernoy, Jerome; Mondon, Sylvain; Bodiguel, Aude; Gouthiere, Laurence; Martin-Phipps, Cecile; Archambault, Sabrina; Joubert, Marion; Madariaga, Nicole; Coste, Marie-Alexandra; Viguie, Vincent; Avner, Paolo; Cassen, Christophe; Guivarch, Celine; Salagnac, Jean-Luc; Carrega, Marie; Menager, Yann

2015-11-01

This report, released in the year of the Paris Climate 2015 summit (COP21), reviews concepts vital for understanding and acting to address climate change at a regional level. Based on the experience of French territories it presents 37 fact-sheets aimed at local players, providing concise and informative access to the most up-to-date knowledge. It also offers feedback on the impacts of climate change, climate policies at a global, European and French level, and economic tools supporting climate change mitigation and adaptation

Analyzing the Response of Climate Perturbations to (Tropical) Cyclones using the WRF Model

Science.gov (United States)

Tewari, M.; Mittal, R.; Radhakrishnan, C.; Cipriani, J.; Watson, C.

2015-12-01

An analysis of global climate models shows considerable changes in the intensity and characteristics of future, warm climate cyclones. At regional scales, deviations in cyclone characteristics are often derived using idealized perturbations in the humidity, temperature and surface conditions. In this work, a more realistic approach is adopted by applying climate perturbations from the Community Climate System Model (CCSM4) to ERA-interim data to generate the initial and boundary conditions for future climate simulations. The climate signal perturbations are generated from the differences in 21 years of mean data from CCSM4 with representative concentration pathways (RCP8.5) for the periods: (a) 2070-2090 (future climate), (b) 2025-2045 (near-future climate) and (c) 1985-2005 (current climate). Four individual cyclone cases are simulated with and without climate perturbations using the Weather Research and Forecasting model with a nested configuration. Each cyclone is characterized by variations in intensity, landfall location, precipitation and societal damage. To calculate societal damage, we use the recently introduced Cyclone Damage Potential (CDP) index evolved from the Willis Hurricane Index (WHI). As CDP has been developed for general societal applications, this work should provide useful insights for resilience analyses and industry (e.g., re-insurance).

Cluster-based analysis of multi-model climate ensembles

Science.gov (United States)

Hyde, Richard; Hossaini, Ryan; Leeson, Amber A.

2018-06-01

Clustering - the automated grouping of similar data - can provide powerful and unique insight into large and complex data sets, in a fast and computationally efficient manner. While clustering has been used in a variety of fields (from medical image processing to economics), its application within atmospheric science has been fairly limited to date, and the potential benefits of the application of advanced clustering techniques to climate data (both model output and observations) has yet to be fully realised. In this paper, we explore the specific application of clustering to a multi-model climate ensemble. We hypothesise that clustering techniques can provide (a) a flexible, data-driven method of testing model-observation agreement and (b) a mechanism with which to identify model development priorities. We focus our analysis on chemistry-climate model (CCM) output of tropospheric ozone - an important greenhouse gas - from the recent Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP). Tropospheric column ozone from the ACCMIP ensemble was clustered using the Data Density based Clustering (DDC) algorithm. We find that a multi-model mean (MMM) calculated using members of the most-populous cluster identified at each location offers a reduction of up to ˜ 20 % in the global absolute mean bias between the MMM and an observed satellite-based tropospheric ozone climatology, with respect to a simple, all-model MMM. On a spatial basis, the bias is reduced at ˜ 62 % of all locations, with the largest bias reductions occurring in the Northern Hemisphere - where ozone concentrations are relatively large. However, the bias is unchanged at 9 % of all locations and increases at 29 %, particularly in the Southern Hemisphere. The latter demonstrates that although cluster-based subsampling acts to remove outlier model data, such data may in fact be closer to observed values in some locations. We further demonstrate that clustering can provide a viable and

Climate change and energy policies in Shanghai: A multilevel governance perspective

International Nuclear Information System (INIS)

Francesch-Huidobro, Maria

2016-01-01

Highlights: • Multilevel governance is a useful framework to understand how resources, tasks and power are distributed for decision making. • Shifts in national climate and energy policy mandate local governments to develop climate change and energy policies. • Local governments have greater autonomy for incorporating climate and energy issues into development goals. • Climate mitigation and energy policy is dominated by hierarchical governance. - Abstract: Despite growing interest in China’s response to climate change and energy security, studies undertaken at the subnational level are rare. In the context of the multilevel governance paradigm, this article examines the governance of climate change and energy policy in Shanghai, a rapidly growing Chinese megacity highly vulnerable to the effects of climate change. Although the energy and carbon intensity of Shanghai’s economy have fallen significantly since China launched its economic reforms, overall carbon emissions in the municipality continue to rise. Through examining the Shanghai case, this article argues that Chinese subnational climate mitigation policy is dominated by hierarchical governance arrangements. Nevertheless, shifts in national climate and energy policy since 2007 have mandated provincial-level governments, including Shanghai, to develop their own climate and energy policies while offering greater local autonomy for incorporating climate and energy issues into development goals: is this attributable to a decentred form of multilevel governance? The article concludes that Shanghai’s climate mitigation and energy policy is dominated by hierarchical governance whereby policies are ‘downloaded’ from the central government. Perspectives for other cities and insights for policymakers are discussed.

Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model.

Science.gov (United States)

Boutrand, Laetitia-Barbollat; Thépot, Amélie; Muther, Charlotte; Boher, Aurélie; Robic, Julie; Guéré, Christelle; Vié, Katell; Damour, Odile; Lamartine, Jérôme

2017-01-01

Human skin is subject to frequent changes in ambient temperature and humidity and needs to cope with these environmental modifications. To decipher the molecular response of human skin to repeated climatic change, a versatile model of skin equivalent subject to "hot-wet" (40°C, 80% relative humidity [RH]) or "cold-dry" (10°C, 40% RH) climatic stress repeated daily was used. To obtain an exhaustive view of the molecular mechanisms elicited by climatic change, large-scale gene expression DNA microarray analysis was performed and modulated function was determined by bioinformatic annotation. This analysis revealed several functions, including epidermal differentiation and extracellular matrix, impacted by repeated variations in climatic conditions. Some of these molecular changes were confirmed by histological examination and protein expression. Both treatments (hot-wet and cold-dry) reduced the expression of genes encoding collagens, laminin, and proteoglycans, suggesting a profound remodeling of the extracellular matrix. Strong induction of the entire family of late cornified envelope genes after cold-dry exposure, confirmed at protein level, was also observed. These changes correlated with an increase in epidermal differentiation markers such as corneodesmosin and a thickening of the stratum corneum, indicating possible implementation of defense mechanisms against dehydration. This study for the first time reveals the complex pattern of molecular response allowing adaption of human skin to repeated change in its climatic environment.

The predictive validity of safety climate.

Science.gov (United States)

Johnson, Stephen E

2007-01-01

Safety professionals have increasingly turned their attention to social science for insight into the causation of industrial accidents. One social construct, safety climate, has been examined by several researchers [Cooper, M. D., & Phillips, R. A. (2004). Exploratory analysis of the safety climate and safety behavior relationship. Journal of Safety Research, 35(5), 497-512; Gillen, M., Baltz, D., Gassel, M., Kirsch, L., & Vacarro, D. (2002). Perceived safety climate, job Demands, and coworker support among union and nonunion injured construction workers. Journal of Safety Research, 33(1), 33-51; Neal, A., & Griffin, M. A. (2002). Safety climate and safety behaviour. Australian Journal of Management, 27, 66-76; Zohar, D. (2000). A group-level model of safety climate: Testing the effect of group climate on microaccidents in manufacturing jobs. Journal of Applied Psychology, 85(4), 587-596; Zohar, D., & Luria, G. (2005). A multilevel model of safety climate: Cross-level relationships between organization and group-level climates. Journal of Applied Psychology, 90(4), 616-628] who have documented its importance as a factor explaining the variation of safety-related outcomes (e.g., behavior, accidents). Researchers have developed instruments for measuring safety climate and have established some degree of psychometric reliability and validity. The problem, however, is that predictive validity has not been firmly established, which reduces the credibility of safety climate as a meaningful social construct. The research described in this article addresses this problem and provides additional support for safety climate as a viable construct and as a predictive indicator of safety-related outcomes. This study used 292 employees at three locations of a heavy manufacturing organization to complete the 16 item Zohar Safety Climate Questionnaire (ZSCQ) [Zohar, D., & Luria, G. (2005). A multilevel model of safety climate: Cross-level relationships between organization and group

New insights into the structural organization of eukaryotic and prokaryotic cytoskeletons using cryo-electron tomography

International Nuclear Information System (INIS)

Kuerner, Julia; Medalia, Ohad; Linaroudis, Alexandros A.; Baumeister, Wolfgang

2004-01-01

Cryo-electron tomography (cryo-ET) is an emerging imaging technology that combines the potential of three-dimensional (3-D) imaging at molecular resolution (<5 nm) with a close-to-life preservation of the specimen. In conjunction with pattern recognition techniques, it enables us to map the molecular landscape inside cells. The application of cryo-ET to intact cells provides novel insights into the structure and the spatial organization of the cytoskeleton in prokaryotic and eukaryotic cells

Enhancing ethical climates in nursing work environments.

Science.gov (United States)

Storch, Janet; Rodney, Patricia; Pauly, Bernadette; Fulton, Thomas Reilly; Stevenson, Lynn; Newton, Lorelei; Makaroff, Kara Schick

2009-03-01

In the current era of providing health care under pressure, considerable strain has been placed on nurses workplaces. Underneath the economic and organizational challenges prevalent in health-care delivery today are important values that shape the ethical climate in workplaces and affect the well-being of nurses, managers, patients and families. In this article, the authors report on the outcomes of Leadership for Ethical Policy and Practice, a three-year participatory action research study involving nurses, managers and other health-care team members in organizations throughout British Columbia. By using an ethics lens to look at problems, participants brought ethical concerns out into the open and were able to gain new insights and identify strategies for action to improve the ethical climate. Nurse leader support was essential for initiating and sustaining projects at six practice sites.

The Green Sahara: Climate Change, Hydrologic History and Human Occupation

Science.gov (United States)

Blom, Ronald G.; Farr, Tom G.; Feynmann, Joan; Ruzmaikin, Alexander; Paillou, Philippe

2009-01-01

Archaeology can provide insight into interactions of climate change and human activities in sensitive areas such as the Sahara, to the benefit of both disciplines. Such analyses can help set bounds on climate change projections, perhaps identify elements of tipping points, and provide constraints on models. The opportunity exists to more precisely constrain the relationship of natural solar and climate interactions, improving understanding of present and future anthropogenic forcing. We are beginning to explore the relationship of human occupation of the Sahara and long-term solar irradiance variations synergetic with changes in atmospheric-ocean circulation patterns. Archaeological and climate records for the last 12 K years are gaining adequate precision to make such comparisons possible. We employ a range of climate records taken over the globe (e.g. Antarctica, Greenland, Cariaco Basin, West African Ocean cores, records from caves) to identify the timing and spatial patterns affecting Saharan climate to compare with archaeological records. We see correlation in changing ocean temperature patterns approx. contemporaneous with drying of the Sahara approx. 6K years BP. The role of radar images and other remote sensing in this work includes providing a geographically comprehensive geomorphic overview of this key area. Such coverage is becoming available from the Japanese PALSAR radar system, which can guide field work to collect archaeological and climatic data to further constrain the climate change chronology and link to models. Our initial remote sensing efforts concentrate on the Gilf Kebir area of Egypt.

A complex relationship between calving glaciers and climate

Science.gov (United States)

Post, A.; O'Neel, S.; Motyka, R.J.; Streveler, G.

2011-01-01

Many terrestrial glaciers are sensitive indicators of past and present climate change as atmospheric temperature and snowfall modulate glacier volume. However, climate interpretations based on glacier behavior require careful selection of representative glaciers, as was recently pointed out for surging and debris-covered glaciers, whose behavior often defies regional glacier response to climate [Yde and Paasche, 2010]. Tidewater calving glaciers (TWGs)mountain glaciers whose termini reach the sea and are generally grounded on the seaflooralso fall into the category of non-representative glaciers because the regional-scale asynchronous behavior of these glaciers clouds their complex relationship with climate. TWGs span the globe; they can be found both fringing ice sheets and in high-latitude regions of each hemisphere. TWGs are known to exhibit cyclic behavior, characterized by slow advance and rapid, unstable retreat, largely independent of short-term climate forcing. This so-called TWG cycle, first described by Post [1975], provides a solid foundation upon which modern investigations of TWG stability are built. Scientific understanding has developed rapidly as a result of the initial recognition of their asynchronous cyclicity, rendering greater insight into the hierarchy of processes controlling regional behavior. This has improved the descriptions of the strong dynamic feedbacks present during retreat, the role of the ocean in TWG dynamics, and the similarities and differences between TWG and ice sheet outlet glaciers that can often support floating tongues.

Molecular breeding for drought tolerance in plants: wheat perspective

International Nuclear Information System (INIS)

Hussain, S.S.; Rivandi, A.; Rivandi, A.

2007-01-01

Wheat (Triticum aestivum L.em Thell.) is the first important and strategic cereal crop for the majority of world,s populations. It is the most important staple food of about two billion people (36% of the world population). Due to industrialization, erosion, urbanization, compaction, and the increase in acidity as a result of fertilization, there is a decrease in the available space for agriculture. Environmental conditions such as increased salinity, drought, and freezing cause adverse effects on the growth and productivity of cereal crops such as wheat (Triticum aestivum L.). Though grown under a wide range of climates and soils, wheat is best adapted to temperate regions. Whether the cropping occurs in the temperate areas or the tropics, both types of environments are affected by global warming and the destabilizing effects that it causes, none more serious than the attendant increased variability in rainfall and temperature. Due to the limited insight into the physiological basis of drought tolerance in wheat, a better understanding of some of the mechanisms that enable the plants to adapt to stress and maintain growth during stress periods would help in breeding for drought tolerance. On the other hand, understanding the genetic and genome organization using molecular markers is of great value for plant breeding purposes. (author)

Public Engagement on Climate Change

Science.gov (United States)

Curry, J.

2011-12-01

Climate change communication is complicated by complexity of the scientific problem, multiple perspectives on the magnitude of the risk from climate change, often acrimonious disputes between scientists, high stakes policy options, and overall politicization of the issue. Efforts to increase science literacy as a route towards persuasion around the need for a policy like cap and trade have failed, because the difficulty that a scientist has in attempting to make sense of the social and political complexity is very similar to the complexity facing the general public as they try to make sense of climate science itself. In this talk I argue for a shift from scientists and their institutions as information disseminators to that of public engagement and enablers of public participation. The goal of engagement is not just to inform, but to enable, motivate and educate the public regarding the technical, political, and social dimensions of climate change. Engagement is a two-way process where experts and decision-makers seek input and learn from the public about preferences, needs, insights, and ideas relative to climate change impacts, vulnerabilities, solutions and policy options. Effective public engagement requires that scientists detach themselves from trying to control what the public does with the acquired knowledge and motivation. The goal should not be to "sell" the public on particular climate change solutions, since such advocacy threatens public trust in scientists and their institutions. Conduits for public engagement include the civic engagement approach in the context of community meetings, and perhaps more significantly, the blogosphere. Since 2006, I have been an active participant in the climate blogosphere, focused on engaging with people that are skeptical of AGW. A year ago, I started my own blog Climate Etc. at judithcurry.com. The demographic that I have focused my communication/engagement activities are the technically educated and scientifically

Insights of asphaltene aggregation mechanism from molecular dynamics simulation

Directory of Open Access Journals (Sweden)

Jennifer De León

2015-01-01

Full Text Available Se estudió el proceso de agregación de asfaltenos utilizando té cnicas de dinámica molecular. Se utilizaron cuatro estructuras diferentes. Las primeras tres moléculas tienen una estructura continental, con núcleos aromáticos condensador, mientras que la cuarta pose e una estructura tipo archipiélago, con pequeños grupos de anillos ar omáticos conectados con cadenas saturadas. Las moléculas fueron construidas de manera atomística, en la cual cada átomo se desc ribe individualmente. Se calcula ron las fuerzas de interacción a 300 K y 200 atm; las fuerzas de Van der W aals y las interacciones elect rostáticas fueron evaluadas separadamente. Se calculó el paráme tro de solubilidad para las cuatro molécu las. Se encontró que las inte racciones de Van der Waals asoc iadas a los anillos aromáticos y las fuerzas electrostáticas ocasionadas princ ipalmente por la presencia de heteroátomos como oxígeno, azufr e y nitrógeno, son igualmente r elevantes en la agregación de moléculas de asfalteno. Para todas las molé culas se encontró que los sistemas de asfaltenos tienen menor e nergía en estado de agregación que en estado monomérico. Para las estruct uras continentales, la presencia de largas cadenas obstruye el proceso de formación de agregados. Para las estructuras tipo archipiélago, la flexibilidad de las moléculas facilita la agregación con ot ras estructuras. La presencia de heteroátomos ocasiona una fuerza repulsiva que dificulta la agregación. El volumen molecular y la energía de c ohesión también son sensibles a la confi guración geométrica y la compos ición de las especies, lo cual afecta el parámetro de solubilidad.

Collaboration pathway(s) using new tools for optimizing `operational' climate monitoring from space

Science.gov (United States)

Helmuth, Douglas B.; Selva, Daniel; Dwyer, Morgan M.

2015-09-01

Consistently collecting the earth's climate signatures remains a priority for world governments and international scientific organizations. Architecting a long term solution requires transforming scientific missions into an optimized robust `operational' constellation that addresses the collective needs of policy makers, scientific communities and global academic users for trusted data. The application of new tools offers pathways for global architecture collaboration. Recent rule-based expert system (RBES) optimization modeling of the intended NPOESS architecture becomes a surrogate for global operational climate monitoring architecture(s). These rulebased systems tools provide valuable insight for global climate architectures, by comparison/evaluation of alternatives and the sheer range of trade space explored. Optimization of climate monitoring architecture(s) for a partial list of ECV (essential climate variables) is explored and described in detail with dialogue on appropriate rule-based valuations. These optimization tool(s) suggest global collaboration advantages and elicit responses from the audience and climate science community. This paper will focus on recent research exploring joint requirement implications of the high profile NPOESS architecture and extends the research and tools to optimization for a climate centric case study. This reflects work from SPIE RS Conferences 2013 and 2014, abridged for simplification30, 32. First, the heavily securitized NPOESS architecture; inspired the recent research question - was Complexity (as a cost/risk factor) overlooked when considering the benefits of aggregating different missions into a single platform. Now years later a complete reversal; should agencies considering Disaggregation as the answer. We'll discuss what some academic research suggests. Second, using the GCOS requirements of earth climate observations via ECV (essential climate variables) many collected from space-based sensors; and accepting their

The molecular basis of disease resistance in higher plants

African Journals Online (AJOL)

xxxxxx

Therefore, manipulating a single transcription factor could have the same effect as manipulating a set of specific genes within the plant. As highlighted above, transgenic plants allow the targeted ... including molecular techniques and genetics will provide insights into pathogen-defense mechanism and subsequent disease ...

Using decision pathway surveys to inform climate engineering policy choices.

Science.gov (United States)

Gregory, Robin; Satterfield, Terre; Hasell, Ariel

2016-01-19

Over the coming decades citizens living in North America and Europe will be asked about a variety of new technological and behavioral initiatives intended to mitigate the worst impacts of climate change. A common approach to public input has been surveys whereby respondents' attitudes about climate change are explained by individuals' demographic background, values, and beliefs. In parallel, recent deliberative research seeks to more fully address the complex value tradeoffs linked to novel technologies and difficult ethical questions that characterize leading climate mitigation alternatives. New methods such as decision pathway surveys may offer important insights for policy makers by capturing much of the depth and reasoning of small-group deliberations while meeting standard survey goals including large-sample stakeholder engagement. Pathway surveys also can help participants to deepen their factual knowledge base and arrive at a more complete understanding of their own values as they apply to proposed policy alternatives. The pathway results indicate more fully the conditional and context-specific nature of support for several "upstream" climate interventions, including solar radiation management techniques and carbon dioxide removal technologies.

NATO Advanced Study Institute on Low Temperature Molecular Spectroscopy

CERN Document Server

1996-01-01

Molecular spectroscopy has achieved rapid and significant progress in recent years, the low temperature techniques in particular having proved very useful for the study of reactive species, phase transitions, molecular clusters and crystals, superconductors and semiconductors, biochemical systems, astrophysical problems, etc. The widening range of applications has been accompanied by significant improvements in experimental methods, and low temperature molecular spectroscopy has been revealed as the best technique, in many cases, to establish the connection between experiment and theoretical calculations. This, in turn, has led to a rapidly increasing ability to predict molecular spectroscopic properties. The combination of an advanced tutorial standpoint with an emphasis on recent advances and new perspectives in both experimental and theoretical molecular spectroscopy contained in this book offers the reader insight into a wide range of techniques, particular emphasis being given to supersonic jet and matri...

Insight into the molecular regulation of the epithelial magnesium channel TRPM6.

NARCIS (Netherlands)

Cao, G.; Hoenderop, J.G.J.; Bindels, R.J.M.

2008-01-01

PURPOSE OF REVIEW: Recent studies have greatly increased our knowledge concerning the molecular mechanisms of renal magnesium handling. This review highlights the functional features of the newly identified transient receptor potential channel melastatin subtype 6 (TRPM6), which forms the gatekeeper

The rise of angiosperm-dominated herbaceous floras: Insights from Ranunculaceae

OpenAIRE

Wei Wang; Li Lin; Xiao-Guo Xiang; Rosa del C. Ortiz; Yang Liu; Kun-Li Xiang; Sheng-Xiang Yu; Yao-Wu Xing; Zhi-Duan Chen

2016-01-01

The rise of angiosperms has been regarded as a trigger for the Cretaceous revolution of terrestrial ecosystems. However, the timeframe of the rise angiosperm-dominated herbaceous floras (ADHFs) is lacking. Here, we used the buttercup family (Ranunculaceae) as a proxy to provide insights into the rise of ADHFs. An integration of phylogenetic, molecular dating, ancestral state inferring, and diversification analytical methods was used to infer the early evolutionary history of Ranunculaceae. We...

Molecular motions that shape the cardiac action potential: Insights from voltage clamp fluorometry.

Science.gov (United States)

Zhu, Wandi; Varga, Zoltan; Silva, Jonathan R

2016-01-01

Very recently, voltage-clamp fluorometry (VCF) protocols have been developed to observe the membrane proteins responsible for carrying the ventricular ionic currents that form the action potential (AP), including those carried by the cardiac Na(+) channel, NaV1.5, the L-type Ca(2+) channel, CaV1.2, the Na(+)/K(+) ATPase, and the rapid and slow components of the delayed rectifier, KV11.1 and KV7.1. This development is significant, because VCF enables simultaneous observation of ionic current kinetics with conformational changes occurring within specific channel domains. The ability gained from VCF, to connect nanoscale molecular movement to ion channel function has revealed how the voltage-sensing domains (VSDs) control ion flux through channel pores, mechanisms of post-translational regulation and the molecular pathology of inherited mutations. In the future, we expect that this data will be of great use for the creation of multi-scale computational AP models that explicitly represent ion channel conformations, connecting molecular, cell and tissue electrophysiology. Here, we review the VCF protocol, recent results, and discuss potential future developments, including potential use of these experimental findings to create novel computational models. Copyright © 2015 Elsevier Ltd. All rights reserved.

Molecular stratigraphy: a new tool for climatic assessment

Science.gov (United States)

Brassell, S. C.; Eglinton, G.; Marlowe, I. T.; Pflaumann, U.; Sarnthein, M.

1986-03-01

Variations in sea-surface temperatures over the past 500,000 years are inferred from the relative abundance behaviour of two organic compounds, C37 alkenones over the upper 8 metres of a sediment core from the eastern equatorial Atlantic. This molecular record, ascribed to contributions from prymnesiophyte algae, correlates well with the variations in the δ18 signal for the calcareous skeletons of certain planktonic foraminifera, thus providing the first demonstration of a new stratigraphical technique, which may be especially valuable where methods based on carbonate δ18 fail.

New insight of Arctic cloud parameterization from regional climate model simulations, satellite-based, and drifting station data

Science.gov (United States)

Klaus, D.; Dethloff, K.; Dorn, W.; Rinke, A.; Wu, D. L.

2016-05-01

Cloud observations from the CloudSat and CALIPSO satellites helped to explain the reduced total cloud cover (Ctot) in the atmospheric regional climate model HIRHAM5 with modified cloud physics. Arctic climate conditions are found to be better reproduced with (1) a more efficient Bergeron-Findeisen process and (2) a more generalized subgrid-scale variability of total water content. As a result, the annual cycle of Ctot is improved over sea ice, associated with an almost 14% smaller area average than in the control simulation. The modified cloud scheme reduces the Ctot bias with respect to the satellite observations. Except for autumn, the cloud reduction over sea ice improves low-level temperature profiles compared to drifting station data. The HIRHAM5 sensitivity study highlights the need for improving accuracy of low-level (<700 m) cloud observations, as these clouds exert a strong impact on the near-surface climate.

Sustainable occupational responses to climate change through lifestyle choices.

Science.gov (United States)

Hocking, Clare; Kroksmark, Ulla

2013-03-01

Abstract Occupational therapists and occupational scientists are increasingly aware of the relationship between occupation and global climate change, with some working to raise awareness of the issues and others proposing that an occupational perspective can make a valuable contribution to understanding and addressing the issues. In this discussion paper the United Nations Global Survey on Sustainable Lifestyles ( 1 ), which reports young adults' beliefs about everyday occupations that have a substantial impact on the environment (food, housekeeping, and transportation) is introduced. The authors argue that the survey findings are a valuable resource for occupational therapists who are concerned about global climate change and work with young adults (age 18-35), providing valuable insights into their concerns and preferences in relation to sustainability. To illustrate the insights contained in the reports, findings from four countries are presented: New Zealand and Sweden, the authors' countries of origin, and the Philippines and Lebanon which have people living in New Zealand and Sweden. Application to individual and community-based interventions to promote more sustainable lifestyles is suggested, along with studies to examine the perspectives of young adults with a disability, as their concerns and sustainability preferences might differ due to the barriers that limit their participation in educational and vocational occupations.

Climate-conscious citizenship in a digital urban setting

Directory of Open Access Journals (Sweden)

Sophie Esmann Andersen

2010-12-01

Full Text Available Climate change has challenged urban life, and as an omnipresent force, Nature sets the agenda for urban living. Using stakeholder theory to conceptualise urban life, we approach Nature as both an omnipresent stakeholder and an issue to be continuously addressed and related to. Adapting the stakeholder focus to relations, stakes and values, we conceptually analyse the digital installation entitled CO2mmitment/CO2nfessions, which was a prominent part of the Aarhus CO2030 exhibition launching the vision of the Danish city of Aarhus to become carbon neutral by the year 2030. In the analysis, we explore how the citizen is framed and invited to enact his/her responsibilities to the natural environment in an urban setting and how the digital mediation facilitates various forms of relations and climate conscious positions, incorporating both narcissistic desires, universal anxiety, moral obligations, ethical virtue and image performance. Statements from the actual confessors/committers exemplify this. Thus, the paper provides insight into understanding the complexity of climate-conscious citizenship as a complex configuration of paradoxical, co-existing ethics and arguments.

Climate-conscious citizenship in a digital urban setting

Directory of Open Access Journals (Sweden)

Sophie Esmann Andersen

2011-06-01

Full Text Available Climate change has challenged urban life, and as an omnipresent force, Nature sets the agenda for urban living. Using stakeholder theory to conceptualise urban life, we approach Nature as both an omnipresent stakeholder and an issue to be continuously addressed and related to. Adapting the stakeholder focus to relations, stakes and values, we conceptually analyse the digital installation entitled CO2mmitment/CO2nfessions, which was a prominent part of the Aarhus CO2030 exhibition launching the vision of the Danish city of Aarhus to become carbon neutral by the year 2030. In the analysis, we explore how the citizen is framed and invited to enact his/her responsibilities to the natural environment in an urban setting and how the digital mediation facilitates various forms of relations and climate conscious positions, incorporating both narcissistic desires, universal anxiety, moral obligations, ethical virtue and image performance. Statements from the actual confessors/committers exemplify this. Thus, the paper provides insight into understanding the complexity of climate-conscious citizenship as a complex configuration of paradoxical, co-existing ethics and arguments.

Structures of Life: The Role of Molecular Structures in Scientists' Work

NARCIS (Netherlands)

Vyas, Dhaval; Kulyk, Olga Anatoliyivna; van der Vet, P.E.; Nijholt, Antinus; van der Veer, Gerrit C.; Jorge, J

2008-01-01

The visual and multidimensional representations like images and graphical structures related to biology provide great insights into understanding the complexities of different organisms. Especially, life scientists use different representations of molecular structures to answer biological questions

Nanosecond and femtosecond mass spectroscopic analysis of a molecular beam produced by the spray-jet technique

International Nuclear Information System (INIS)

Yamada, Toshiki; Shinohara, Hidenori; Kamikado, Toshiya; Okuno, Yoshishige; Suzuki, Hitoshi; Mashiko, Shinro; Yokoyama, Shiyoshi

2008-01-01

The spray-jet molecular beam apparatus enabled us to produce a molecular beam of non-volatile molecules under high vacuum from a sprayed mist of sample solutions. The apparatus has been used in spectroscopic studies and as a means of molecular beam deposition. We analyzed the molecular beam, consisting of non-volatile, solvent, and carrier-gas molecules, by using femtosecond- and nanosecond- laser mass spectroscopy. The information thus obtained provided insight into the molecular beam produced by the spray-jet technique

Diverging effects of isotopic fractionation upon molecular diffusion of noble gases in water: mechanistic insights through ab initio molecular dynamics simulations.

Science.gov (United States)

Pinto de Magalhães, Halua; Brennwald, Matthias S; Kipfer, Rolf

2017-03-22

Atmospheric noble gases are routinely used as natural tracers to analyze gas transfer processes in aquatic systems. Their isotopic ratios can be employed to discriminate between different physical transport mechanisms by comparison to the unfractionated atmospheric isotope composition. In many applications of aquatic systems molecular diffusion was thought to cause a mass dependent fractionation of noble gases and their isotopes according to the square root ratio of their masses. However, recent experiments focusing on isotopic fractionation within a single element challenged this broadly accepted assumption. The determined fractionation factors of Ne, Ar, Kr and Xe isotopes revealed that only Ar follows the prediction of the so-called square root relation, whereas within the Ne, Kr and Xe elements no mass-dependence was found. The reason for this unexpected divergence of Ar is not yet understood. The aim of our computational exercise is to establish the molecular-resolved mechanisms behind molecular diffusion of noble gases in water. We make the hypothesis that weak intermolecular interactions are relevant for the dynamical properties of noble gases dissolved in water. Therefore, we used ab initio molecular dynamics to explicitly account for the electronic degrees of freedom. Depending on the size and polarizability of the hydrophobic particles such as noble gases, their motion in dense and polar liquids like water is subject to different diffusive regimes: the inter-cavity hopping mechanism of small particles (He, Ne) breaks down if a critical particle size achieved. For the case of large particles (Kr, Xe), the motion through the water solvent is governed by mass-independent viscous friction leading to hydrodynamical diffusion. Finally, Ar falls in between the two diffusive regimes, where particle dispersion is propagated at the molecular collision time scale of the surrounding water molecules.

Evolutionary ARMS Race: Antimalarial Resistance Molecular Surveillance.

Science.gov (United States)

Prosser, Christiane; Meyer, Wieland; Ellis, John; Lee, Rogan

2018-04-01

Molecular surveillance of antimalarial drug resistance markers has become an important part of resistance detection and containment. In the current climate of multidrug resistance, including resistance to the global front-line drug artemisinin, there is a consensus to upscale molecular surveillance. The most salient limitation to current surveillance efforts is that skill and infrastructure requirements preclude many regions. This includes sub-Saharan Africa, where Plasmodium falciparum is responsible for most of the global malaria disease burden. New molecular and data technologies have emerged with an emphasis on accessibility. These may allow surveillance to be conducted in broad settings where it is most needed, including at the primary healthcare level in endemic countries, and extending to the village health worker. Copyright © 2018 Elsevier Ltd. All rights reserved.

Future Projections of Fire Occurrence in Brazil Using EC-Earth Climate Model

Directory of Open Access Journals (Sweden)

Patrícia Silva

Full Text Available Abstract Fire has a fundamental role in the Earth system as it influences global and local ecosystem patterns and processes, such as vegetation distribution and structure, the carbon cycle and climate. Since, in the global context, Brazil is one of the regions with higher fire activity, an assessment is here performed of the sensitivity of the wildfire regime in Brazilian savanna and shrubland areas to changes in regional climate during the 21st Century, for an intermediate scenario (RCP4.5 of climate change. The assessment is based on a spatial and temporal analysis of a meteorological fire danger index specifically developed for Brazilian biomes, which was evaluated based on regional climate simulations of temperature, relative humidity and precipitation using the Rossby Centre Regional Climate Model (RCA4 forced by the EC-Earth earth system model. Results show a systematic increase in the extreme levels of fire danger throughout the 21st Century that mainly results from the increase in maximum daily temperature, which rises by about 2 °C between 2005 and 2100. This study provides new insights about projected fire activity in Brazilian woody savannas associated to climate change and is expected to benefit the user community, from governmental policies to land management and climate researches.

Catchment Classification: Connecting Climate, Structure and Function

Science.gov (United States)

Sawicz, K. A.; Wagener, T.; Sivapalan, M.; Troch, P. A.; Carrillo, G. A.

2010-12-01

Hydrology does not yet possess a generally accepted catchment classification framework. Such a classification framework needs to: [1] give names to things, i.e. the main classification step, [2] permit transfer of information, i.e. regionalization of information, [3] permit development of generalizations, i.e. to develop new theory, and [4] provide a first order environmental change impact assessment, i.e., the hydrologic implications of climate, land use and land cover change. One strategy is to create a catchment classification framework based on the notion of catchment functions (partitioning, storage, and release). Results of an empirical study presented here connects climate and structure to catchment function (in the form of select hydrologic signatures), based on analyzing over 300 US catchments. Initial results indicate a wide assortment of signature relationships with properties of climate, geology, and vegetation. The uncertainty in the different regionalized signatures varies widely, and therefore there is variability in the robustness of classifying ungauged basins. This research provides insight into the controls of hydrologic behavior of a catchment, and enables a classification framework applicable to gauged and ungauged across the study domain. This study sheds light on what we can expect to achieve in mapping climate, structure and function in a top-down manner. Results of this study complement work done using a bottom-up physically-based modeling framework to generalize this approach (Carrillo et al., this session).

Culture, climate change and farm-level groundwater management: An Australian case study

Science.gov (United States)

Sanderson, Matthew R.; Curtis, Allen L.

2016-05-01

Cultural factors - values, beliefs, and norms - provide important insights into the environmental attitudes, risk perceptions, and behaviors of the general population. Little is known, however, about the ostensibly complex relationships linking those elements of culture to climate change risk perceptions, especially in the context of farm level decision in the ground water context. This paper addresses that gap through an analysis of survey data provided by irrigators in the Namoi catchment of Australia's Murray-Darling Basin. We use Values-Beliefs-Norms theory to construct multivariate models of the relationship between ground water irrigators' interpretations of climate change risks and their implementation of adaptive water conservation practices. Results indicate that these cultural factors are important explanations of irrigators' climate change risk perceptions, and these risk perceptions are related to adaptive ground water management strategies at the farm level. The implications of the findings are discussed for research on the culture-environment nexus and for outreach designed to encourage agricultural adaptations to climate change.

Far-infrared spectroscopy of lanthanide-based molecular magnetic materials

Energy Technology Data Exchange (ETDEWEB)

Haas, Sabrina

2015-05-13

This thesis demonstrates the applicability of far-infrared spectroscopy for the study of the crystal-field splitting of lanthanides in single-molecular magnetic materials. The far-infrared studies of three different kinds of single-molecular-magnetic materials, a single-ion magnet, a single-chain magnet and an exchange-coupled cluster, yielded a deeper understanding of the crystal-field splitting of the lanthanides in these materials. In addition, our results offered the opportunity to gain a deeper insight into the relaxation processes of these materials.

"Days of future passed" - climate change and carbon cycle history (Jean Baptiste Lamarck Medal Lecture)

Science.gov (United States)

Weissert, Helmut

2013-04-01

With the beginning of the fossil fuel age in the 19th century mankind has become an important geological agent on a global scale. For the first time in human history action of man has an impact on global biogeochemical cycles. Increasing CO2 concentrations will result in a perturbation of global carbon cycling coupled with climate change. Investigations of past changes in carbon cycling and in climate will improve our predictions of future climate. Increasing atmospheric CO2 concentrations will drive climate into a mode of operation, which may resemble climate conditions in the deep geological past. Pliocene climate will give insight into 400ppm world with higher global sea level than today. Doubling of pre-industrial atmospheric CO2 levels will shift the climate system into a state resembling greenhouse climate in the Early Cenozoic or even in the Cretaceous. Carbon isotope geochemistry serves as tool for tracing the pathway of the carbon cycle through geological time. Globally registered negative C-isotope anomalies in the C-isotope record are interpreted as signatures of rapid addition (103 to a few 104 years) of CO2 to the ocean-atmosphere system. Positive C-isotope excursions following negative spikes record the slow post-perturbation recovery of the biosphere at time scales of 105 to 106 years. Duration of C-cycle perturbations in earth history cannot be directly compared with rapid perturbation characterizing the Anthropocene. However, the investigation of greenhouse pulses in the geological past provides insight into different climate states, it allows to identify tipping points in past climate systems and it offers the opportunity to learn about response reactions of the biosphere to rapid changes in global carbon cycling. Sudden injection of massive amounts of carbon dioxide into the atmosphere is recorded in C-isotope record of the Early Cretaceous. The Aptian carbon cycle perturbation triggered changes in temperature and in global hydrological cycling

Avoiding maladaptation to climate change: towards guiding principles

International Nuclear Information System (INIS)

Magnan, Alexandre

2013-08-01

The recent publication of the Physical Science Basis volume of IPCC's Fifth Assessment Report reaffirms an already known conclusion: even drastic reductions of global greenhouse gas emissions will be insufficient to avoid some of the impacts of climate change, and is becoming increasingly clear that the temperature increase by the end of the century is likely to exceed the official target of +2 deg. C. Urgent efforts are thus more than ever needed to support socio-ecological systems threatened by climate change, but how to make adaptation happen on the ground remains vague. Consequently, there is a real risk that climate funding may support initiatives that are actually harmful for the socio-ecological systems, i.e. that foster adaptation in the short-term but insidiously affect systems' long-term vulnerability and/or adaptive capacity to climate change. This generally defines 'mal-adaptation', and this paper affirms that avoiding mal-adaptation is a first key concrete step towards adaptation in a broader sense. Focusing on coastal areas at a local scale and with the aim of providing insights to help avoiding mal-adaptation to climate change on the ground, this paper develops eleven practice-oriented guidelines that address the environmental, socio-cultural and economic dimensions of adaptation initiatives (policies, plans, projects). Based upon this, it affirms that the more guidelines an initiative addresses, the lower will be the risk of mal-adaptation. Together, these guidelines and this assumption constitute the 'Assessment framework' for approaching mal-adaptation to climate change at a local level. (author)

Molecular Diagnostics for Soilborne Fungal Pathogens

Directory of Open Access Journals (Sweden)

E.J. Paplomatas

2004-08-01

Full Text Available Several classical approaches have been developed to detect and identify soil fungal inhabitants through the years. Selective media have been devised to exclude the large number of soil organisms and allow growth of target fungi. However the advent of molecular biology has offered a number of revolutionary insights into the detection and enumeration of soilborne fungal pathogens and also has started to provide information on the identification of unknown species from DNA sequences. This review paper focuses on the application of various molecular techniques in the detection, identification, characterization and quantification of soilborne fungal plant pathogens. This is based on information from the literature and is combined with personal research findings of the author.

Molecular medicine: a path towards a personalized medicine.

Science.gov (United States)

Miranda, Debora Marques de; Mamede, Marcelo; Souza, Bruno Rezende de; Almeida Barros, Alexandre Guimarães de; Magno, Luiz Alexandre; Alvim-Soares, Antônio; Rosa, Daniela Valadão; Castro, Célio José de; Malloy-Diniz, Leandro; Gomez, Marcus Vinícius; Marco, Luiz Armando De; Correa, Humberto; Romano-Silva, Marco Aurélio

2012-03-01

Psychiatric disorders are among the most common human illnesses; still, the molecular and cellular mechanisms underlying their complex pathophysiology remain to be fully elucidated. Over the past 10 years, our group has been investigating the molecular abnormalities in major signaling pathways involved in psychiatric disorders. Recent evidences obtained by the Instituto Nacional de Ciência e Tecnologia de Medicina Molecular (National Institute of Science and Technology - Molecular Medicine, INCT-MM) and others using behavioral analysis of animal models provided valuable insights into the underlying molecular alterations responsible for many complex neuropsychiatric disorders, suggesting that "defects" in critical intracellular signaling pathways have an important role in regulating neurodevelopment, as well as in pathophysiology and treatment efficacy. Resources from the INCT have allowed us to start doing research in the field of molecular imaging. Molecular imaging is a research discipline that visualizes, characterizes, and quantifies the biologic processes taking place at cellular and molecular levels in humans and other living systems through the results of image within the reality of the physiological environment. In order to recognize targets, molecular imaging applies specific instruments (e.g., PET) that enable visualization and quantification in space and in real-time of signals from molecular imaging agents. The objective of molecular medicine is to individualize treatment and improve patient care. Thus, molecular imaging is an additional tool to achieve our ultimate goal.

Multi-level molecular modelling for plasma medicine

International Nuclear Information System (INIS)

Bogaerts, Annemie; Khosravian, Narjes; Van der Paal, Jonas; Verlackt, Christof C W; Yusupov, Maksudbek; Kamaraj, Balu; Neyts, Erik C

2016-01-01

Modelling at the molecular or atomic scale can be very useful for obtaining a better insight in plasma medicine. This paper gives an overview of different atomic/molecular scale modelling approaches that can be used to study the direct interaction of plasma species with biomolecules or the consequences of these interactions for the biomolecules on a somewhat longer time-scale. These approaches include density functional theory (DFT), density functional based tight binding (DFTB), classical reactive and non-reactive molecular dynamics (MD) and united-atom or coarse-grained MD, as well as hybrid quantum mechanics/molecular mechanics (QM/MM) methods. Specific examples will be given for three important types of biomolecules, present in human cells, i.e. proteins, DNA and phospholipids found in the cell membrane. The results show that each of these modelling approaches has its specific strengths and limitations, and is particularly useful for certain applications. A multi-level approach is therefore most suitable for obtaining a global picture of the plasma–biomolecule interactions. (paper)

Applications of molecular replacement to G protein-coupled receptors

International Nuclear Information System (INIS)

Kruse, Andrew C.; Manglik, Aashish; Kobilka, Brian K.; Weis, William I.

2013-01-01

The use of molecular replacement in solving the structures of G protein-coupled receptors is discussed, with specific examples being described in detail. G protein-coupled receptors (GPCRs) are a large class of integral membrane proteins involved in regulating virtually every aspect of human physiology. Despite their profound importance in human health and disease, structural information regarding GPCRs has been extremely limited until recently. With the advent of a variety of new biochemical and crystallographic techniques, the structural biology of GPCRs has advanced rapidly, offering key molecular insights into GPCR activation and signal transduction. To date, almost all GPCR structures have been solved using molecular-replacement techniques. Here, the unique aspects of molecular replacement as applied to individual GPCRs and to signaling complexes of these important proteins are discussed

Congenital heart disease and genetic syndromes: new insights into molecular mechanisms.

Science.gov (United States)

Calcagni, Giulio; Unolt, Marta; Digilio, Maria Cristina; Baban, Anwar; Versacci, Paolo; Tartaglia, Marco; Baldini, Antonio; Marino, Bruno

2017-09-01

Advances in genetics allowed a better definition of the role of specific genetic background in the etiology of syndromic congenital heart defects (CHDs). The identification of a number of disease genes responsible for different syndromes have led to the identification of several transcriptional regulators and signaling transducers and modulators that are critical for heart morphogenesis. Understanding the genetic background of syndromic CHDs allowed a better characterization of the genetic basis of non-syndromic CHDs. In this sense, the well-known association of typical CHDs in Down syndrome, 22q11.2 microdeletion and Noonan syndrome represent paradigms as chromosomal aneuploidy, chromosomal microdeletion and intragenic mutation, respectively. Area covered: For each syndrome the anatomical features, distinctive cardiac phenotype and molecular mechanisms are discussed. Moreover, the authors include recent genetic findings that may shed light on some aspects of still unclear molecular mechanisms of these syndromes. Expert commentary: Further investigations are needed to enhance the translational approach in the field of genetics of CHDs. When there is a well-established definition of genotype-phenotype (reverse medicine) and genotype-prognosis (predictive and personalized medicine) correlations, hopefully preventive medicine will make its way in this field. Subsequently a reduction will be achieved in the morbidity and mortality of children with CHDs.

Climate-Driven Risk of Large Fire Occurrence in the Western United States, 1500 to 2003

Science.gov (United States)

Crockett, J.; Westerling, A. L.

2017-12-01

Spatially comprehensive fire climatology has provided managers with tools to understand thecauses and consequences of large forest wildfires, but a paleoclimate context is necessary foranticipating the trajectory of future climate-fire relationships. Although accumulated charcoalrecords and tree scars have been utilized in high resolution, regional fire reconstructions, there isuncertainty as to how current climate-fire relationships of the western United States (WUS) fitwithin the natural long-term variability. While contemporary PDSI falls within the naturalvariability of the past, contemporary temperatures skew higher. Here, we develop a WUSfire reconstruction by applying climate-fire-topography model built on the 1972 to 2003 periodto the past 500 years, validated by recently updated fire-scar histories from WUS forests. Theresultant narrative provides insight into changing climate-fire relationships during extendedperiods of high aridity and temperature, providing land managers with historical precedent toeffectively anticipate disturbances during future climate change.

The sociological imagination in a time of climate change

Science.gov (United States)

Norgaard, Kari Marie

2018-04-01

Despite rising calls for social science knowledge in the face of climate change, too few sociologists have been engaged in the conversations about how we have arrived at such perilous climatic circumstances, or how society can change course. With its attention to the interactive dimensions of social order between individuals, social norms, cultural systems and political economy, the discipline of sociology is uniquely positioned to be an important leader in this conversation. In this paper I suggest that in order to understand and respond to climate change we need two kinds of imagination: 1) to see the relationships between human actions and their impacts on earth's biophysical system (ecological imagination) and 2) to see the relationships within society that make up this environmentally damaging social structure (sociological imagination). The scientific community has made good progress in developing our ecological imagination but still need to develop a sociological imagination. The application of a sociological imagination allows for a powerfully reframing of four key problems in the current interdisciplinary conversation on climate change: why climate change is happening, how we are being impacted, why we have failed to successfully respond so far, and how we might be able to effectively do so. I visit each of these four questions describing the current understanding and show the importance of the sociological imagination and other insights from the field of sociology. I close with reflections on current limitations in sociology's potential to engage climate change and the Anthropocene.

Molecular Basis of Clay Mineral Structure and Dynamics in Subsurface Engineering Applications

Science.gov (United States)

Cygan, R. T.

2015-12-01

Clay minerals and their interfaces play an essential role in many geochemical, environmental, and subsurface engineering applications. Adsorption, dissolution, precipitation, nucleation, and growth mechanisms, in particular, are controlled by the interplay of structure, thermodynamics, kinetics, and transport at clay mineral-water interfaces. Molecular details of these processes are typically beyond the sensitivity of experimental and analytical methods, and therefore require accurate models and simulations. Also, basal surfaces and interlayers of clay minerals provide constrained interfacial environments to facilitate the evaluation of these complex processes. We have developed and used classical molecular and quantum methods to examine the complex behavior of clay mineral-water interfaces and dynamics of interlayer species. Bulk structures, swelling behavior, diffusion, and adsorption processes are evaluated and compared to experimental and spectroscopic findings. Analysis of adsorption mechanisms of radionuclides on clay minerals provides a scientific basis for predicting the suitability of engineered barriers associated with nuclear waste repositories and the fate of contaminants in the environment. Similarly, the injection of supercritical carbon dioxide into geological reservoirs—to mitigate the impact of climate change—is evaluated by molecular models of multi-fluid interactions with clay minerals. Molecular dynamics simulations provide insights into the wettability of different fluids—water, electrolyte solutions, and supercritical carbon dioxide—on clay surfaces, and which ultimately affects capillary fluid flow and the integrity of shale caprocks. This work is supported as part of Center for Frontiers of Subsurface Energy Security, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science and by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Geosciences Research Program

A Social Science Guide for Communication on Climate Change

Science.gov (United States)

St John, C.; Marx, S.; Markowitz, E.

2014-12-01

Researchers from the Center for Research on Environmental Decisions (CRED) published "The Psychology of Climate Change Communication: A Guide for Scientists, Journalists, Educators, Political Aides, and the Interested Public" in 2009. This landmark guide provided climate change communicators a synthesis of the social science research that was pertinent to understanding how people think about climate change and how the practice could be improved. In the fall of 2014 this guide will be rereleased, with a new title, and in a partnership between CRED and ecoAmerica. The updated guide addresses how and why Americans respond in certain ways to climate change and explains how communicators can apply best practices to their own work. The guide, which includes research from a range of social science fields including psychology, anthropology, communications, and behavioral economics, is designed to be useful for experienced and novice communicators alike. Included in the guide are strategies to boost engagement, common mistakes to avoid, and best practices that organizations around the world have used to meaningfully engage individuals and groups on climate change. The proposed presentation will provide an overview of the main findings and tips from the 2014 climate change communication guide. It will provide a deeper look at a few of the key points that are crucial for increasing audience engagement with climate change including understanding how identity shapes climate change, how to lead with solutions, and how to bring the impacts of climate change close to home. It will highlight tips for motivating positive behavior change that will lead people down the path toward solutions. Finally, it will address the benefits and challenges associated with producing a communication guide and insight into synthesizing social science research findings into a usable format for a variety of audiences.

Molecular electronics with single molecules in solid-state devices

DEFF Research Database (Denmark)

Moth-Poulsen, Kasper; Bjørnholm, Thomas

2009-01-01

The ultimate aim of molecular electronics is to understand and master single-molecule devices. Based on the latest results on electron transport in single molecules in solid-state devices, we focus here on new insights into the influence of metal electrodes on the energy spectrum of the molecule...

An empirical perspective for understanding climate change impacts in Switzerland

Science.gov (United States)

Henne, Paul; Bigalke, Moritz; Büntgen, Ulf; Colombaroli, Daniele; Conedera, Marco; Feller, Urs; Frank, David; Fuhrer, Jürg; Grosjean, Martin; Heiri, Oliver; Luterbacher, Jürg; Mestrot, Adrien; Rigling, Andreas; Rössler, Ole; Rohr, Christian; Rutishauser, This; Schwikowski, Margit; Stampfli, Andreas; Szidat, Sönke; Theurillat, Jean-Paul; Weingartner, Rolf; Wilcke, Wolfgan; Tinner, Willy

2018-01-01

Planning for the future requires a detailed understanding of how climate change affects a wide range of systems at spatial scales that are relevant to humans. Understanding of climate change impacts can be gained from observational and reconstruction approaches and from numerical models that apply existing knowledge to climate change scenarios. Although modeling approaches are prominent in climate change assessments, observations and reconstructions provide insights that cannot be derived from simulations alone, especially at local to regional scales where climate adaptation policies are implemented. Here, we review the wealth of understanding that emerged from observations and reconstructions of ongoing and past climate change impacts in Switzerland, with wider applicability in Europe. We draw examples from hydrological, alpine, forest, and agricultural systems, which are of paramount societal importance, and are projected to undergo important changes by the end of this century. For each system, we review existing model-based projections, present what is known from observations, and discuss how empirical evidence may help improve future projections. A particular focus is given to better understanding thresholds, tipping points and feedbacks that may operate on different time scales. Observational approaches provide the grounding in evidence that is needed to develop local to regional climate adaptation strategies. Our review demonstrates that observational approaches should ideally have a synergistic relationship with modeling in identifying inconsistencies in projections as well as avenues for improvement. They are critical for uncovering unexpected relationships between climate and agricultural, natural, and hydrological systems that will be important to society in the future.

Perception of Transfer Climate Factors in the Macro and Micro Organizational Work Environment

Science.gov (United States)

Diggs, Byron Kenneth

2011-01-01

This qualitative study was designed to provide insight on the perceived transfer climate factors in the macro and micro organizational work environment that may influence an employee's willingness to transfer what was learned in a training program to the job. More specifically, the purpose of the study was to delineate descriptive patterns and…

Changing climate, changing forests: the impacts of climate change on forests of the northeastern United States and eastern Canada

Science.gov (United States)

Rustad, Lindsey; Campbell, John; Dukes, Jeffrey S.; Huntington, Thomas; Lambert, Kathy Fallon; Mohan, Jacqueline; Rodenhouse, Nicholas

2012-01-01

Decades of study on climatic change and its direct and indirect effects on forest ecosystems provide important insights for forest science, management, and policy. A synthesis of recent research from the northeastern United States and eastern Canada shows that the climate of the region has become warmer and wetter over the past 100 years and that there are more extreme precipitation events. Greater change is projected in the future. The amount of projected future change depends on the emissions scenarios used. Tree species composition of northeast forests has shifted slowly in response to climate for thousands of years. However, current human-accelerated climate change is much more rapid and it is unclear how forests will respond to large changes in suitable habitat. Projections indicate significant declines in suitable habitat for spruce-fir forests and expansion of suitable habitat for oak-dominated forests. Productivity gains that might result from extended growing seasons and carbon dioxide and nitrogen fertilization may be offset by productivity losses associated with the disruption of species assemblages and concurrent stresses associated with potential increases in atmospheric deposition of pollutants, forest fragmentation, and nuisance species. Investigations of links to water and nutrient cycling suggest that changes in evapotranspiration, soil respiration, and mineralization rates could result in significant alterations of key ecosystem processes. Climate change affects the distribution and abundance of many wildlife species in the region through changes in habitat, food availability, thermal tolerances, species interactions such as competition, and susceptibility to parasites and disease. Birds are the most studied northeastern taxa. Twenty-seven of the 38 bird species for which we have adequate long-term records have expanded their ranges predominantly in a northward direction. There is some evidence to suggest that novel species, including pests and

How to manage uncertainty in future Life Cycle Assessment (LCA) scenarios addressing the effect of climate change in crop production

DEFF Research Database (Denmark)

Niero, Monia; Ingvordsen, Cathrine Heinz; Bagger Jørgensen, Rikke

2015-01-01

When Life Cycle Assessment (LCA) is used to provide insights on how to pursue future food demand, it faces the challenge to describe scenarios of the future in which the environmental impacts occur. In the case of future crop production, the effects of climate change should be considered. In this......When Life Cycle Assessment (LCA) is used to provide insights on how to pursue future food demand, it faces the challenge to describe scenarios of the future in which the environmental impacts occur. In the case of future crop production, the effects of climate change should be considered....... In this context, the objectives of this paper are two-fold: (i) to recommend an approach to deal with uncertainty in scenario analysis for LCA of crop production in a changed climate, when the goal of the study is to suggest strategies for adaptation of crop cultivation practices towards low environmental impacts...... climate, soil, water loss and production parameters. Secondly, the handling of these factors in the inventory modeling is discussed and finally implemented in the case study. Our approach follows a 3-step procedure consisting of: (1) definition of a baseline scenario at the Life Cycle Inventory (LCI...

Analysis of Swedish Forest Owners' Information and Knowledge-Sharing Networks for Decision-Making: Insights for Climate Change Communication and Adaptation

Science.gov (United States)

André, Karin; Baird, Julia; Gerger Swartling, Åsa; Vulturius, Gregor; Plummer, Ryan

2017-06-01

To further the understanding of climate change adaptation processes, more attention needs to be paid to the various contextual factors that shape whether and how climate-related knowledge and information is received and acted upon by actors involved. This study sets out to examine the characteristics of forest owners' in Sweden, the information and knowledge-sharing networks they draw upon for decision-making, and their perceptions of climate risks, their forests' resilience, the need for adaptation, and perceived adaptive capacity. By applying the concept of ego-network analysis, the empirical data was generated by a quantitative survey distributed to 3000 private forest owners' in Sweden in 2014 with a response rate of 31%. The results show that there is a positive correlation, even though it is generally weak, between forest owner climate perceptions and (i) network features, i.e. network size and heterogeneity, and (ii) presence of certain alter groups (i.e. network members or actors). Results indicate that forest owners' social networks currently serve only a minimal function of sharing knowledge of climate change and adaptation. Moreover, considering the fairly infrequent contact between respondents and alter groups, the timing of knowledge sharing is important. In conclusion we suggest those actors that forest owners' most frequently communicate with, especially forestry experts providing advisory services (e.g. forest owner associations, companies, and authorities) have a clear role to communicate both the risks of climate change and opportunities for adaptation. Peers are valuable in connecting information about climate risks and adaptation to the actual forest property.

First insight into dead wood protistan diversity: a molecular sampling of bright-spored Myxomycetes (Amoebozoa, slime-moulds) in decaying beech logs.

Science.gov (United States)

Clissmann, Fionn; Fiore-Donno, Anna Maria; Hoppe, Björn; Krüger, Dirk; Kahl, Tiemo; Unterseher, Martin; Schnittler, Martin

2015-06-01

Decaying wood hosts a large diversity of seldom investigated protists. Environmental sequencing offers novel insights into communities, but has rarely been applied to saproxylic protists. We investigated the diversity of bright-spored wood-inhabiting Myxomycetes by environmental sequencing. Myxomycetes have a complex life cycle culminating in the formation of mainly macroscopic fruiting bodies, highly variable in shape and colour that are often found on decaying logs. Our hypothesis was that diversity of bright-spored Myxomycetes would increase with decay. DNA was extracted from wood chips collected from 17 beech logs of varying decay stages from the Hainich-Dün region in Central Germany. We obtained 260 partial small subunit ribosomal RNA gene sequences of bright-spored Myxomycetes that were assembled into 29 OTUs, of which 65% were less than 98% similar to those in the existing database. The OTU richness revealed by molecular analysis surpassed that of a parallel inventory of fruiting bodies. We tested several environmental variables and identified pH, rather than decay stage, as the main structuring factor of myxomycete distribution. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Micro RNAs as molecular markers of glioblastoma multiform

Energy Technology Data Exchange (ETDEWEB)

Farace, M G [Department Experimental Medicine and Biochemical Sciences, University of Tor Vergata, Rome (Italy); Finocchiaro, G [Istituto Neurologico Besta, Milan (Italy); Ricci Vitiani, L [Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome (Italy)

2009-07-01

The aim of this project was to unravel the role that miR-221 and miR-222, of which we had already demonstrated the specific differential expression in glioblastoma multiforme compared to normal brain, play in the control of cell proliferation, with the ultimate goal to provide new insights in the molecular basis of cancer. The results of our research allowed to identify an important molecular target for miRNA-221 and miR-222, highly expressed in glioblastoma multiforme tissues and cell lines, and to precisely recognize the mRNA regions responsible for this regulation.

Micro RNAs as molecular markers of glioblastoma multiform

International Nuclear Information System (INIS)

Farace, M.G.; Finocchiaro, G.; Ricci Vitiani, L.

2009-01-01

The aim of this project was to unravel the role that miR-221 and miR-222, of which we had already demonstrated the specific differential expression in glioblastoma multiforme compared to normal brain, play in the control of cell proliferation, with the ultimate goal to provide new insights in the molecular basis of cancer. The results of our research allowed to identify an important molecular target for miRNA-221 and miR-222, highly expressed in glioblastoma multiforme tissues and cell lines, and to precisely recognize the mRNA regions responsible for this regulation

Insights into soil carbon dynamics across climatic and geologic gradients from temporally-resolved radiocarbon measurements

Science.gov (United States)

van der Voort, T. S.; Hagedorn, F.; Mannu, U.; Walthert, L.; McIntyre, C.; Eglinton, T. I.

2016-12-01

Soil carbon constitutes the largest terrestrial reservoir of organic carbon, and therefore quantifying soil organic matter dynamics (carbon turnover, stocks and fluxes) across spatial gradients is essential for an understanding of the carbon cycle and the impacts of global change. In particular, links between soil carbon dynamics and different climatic and compositional factors remains poorly understood. Radiocarbon constitutes a powerful tool for unraveling soil carbon dynamics. Temporally-resolved radiocarbon measurements, which take advantage of "bomb-radiocarbon"-driven changes in atmospheric 14C, enable further constraints to be placed on C turnover times. These in turn can yield more precise flux estimates for both upper and deeper soil horizons. This project combines bulk radiocarbon measurements on a suite of soil profiles spanning strong climatic (MAT 1.3-9.2°C, MAP 600 to 2100 mm m-2y-1) and geologic gradients with a more in-depth approach for a subset of locations. For this subset, temporal and carbon-fraction specific radiocarbon data has been acquired for both topsoil and deeper soils. These well-studied sites are part of the Long-Term Forest Ecosystem Research (LWF) program of the Swiss Federal Institute for Forest, Snow and Landscape research (WSL). Resulting temporally-resolved turnover estimates are coupled to carbon stocks, fluxes across this wide range of forest ecosystems and are examined in the context of environmental drivers (temperature, precipitation, primary production and soil moisture) as well as composition (sand, silt and clay content). Statistical analysis on the region-scale - correlating radiocarbon signature with climatic variables such as temperature, precipitation, primary production and elevation - indicates that composition rather than climate is a key driver of ­­Δ14C signatures. Estimates of carbon turnover, stocks and fluxes derived from temporally-resolved measurements highlight the pivotal role of soil moisture as a

Evidence of Ice Age humans in Beringia: new insights on the peopling of the Americas

Science.gov (United States)

Vachula, R. S.; Longo, W. M.; Daniels, W.; Dee, S.; Russell, J. M.; Huang, Y.

2017-12-01

The American continents were the final frontier of the global proliferation of Homo sapiens. The Bering Land Bridge that once connected Eurasia and North America, is widely accepted as the corridor for this human migration. However, the timeline, pathway, and environmental impact of human arrival to these previously unpeopled lands remain contentious in part due to a sparsity of physical archaeological or paleoenvironmental evidence. Recent conflicting genetic evidence suggests that humans lived in Beringia for an extended period of time (ca. 15,000 years) and did not diverge from Asian populations prior to 23,000 years before present (BP). The genetic evidence lacks supporting physical data, is prone to uncertainties inherent to molecular clocks, and thus further occludes our understanding of this segment of human history. Using a multi-disciplinary approach incorporating paleoecological and organic geochemical analyses of a lacustrine sediment record, and climate modelling methodologies, we present evidence of prolonged human-caused environmental disturbance in modern-day northern Alaska and show that humans were present in this area as early as 32,000 years ago. We measured fecal biomarkers preserved in the Lake E5 sediment archive to confirm prolonged human presence in the watershed, and find evidence of contemporaneous burning, as inferred from macroscopic charcoal and polycyclic aromatic hydrocarbons. Climate simulations show that natural lightning ignitions were suppressed during the period of greatest fire frequency, and so we infer that humans likely played an important role in fire ignitions. We thus present new physical evidence of sustained human presence in eastern Beringia throughout the last Glacial, which reorients our understanding of the human migration to the Americas. Furthermore, our research offers new insight into the ecology of the mammoth steppe and the role of humans in the extinction of the Pleistocene megafauna.

Big climate data analysis

Science.gov (United States)

Mudelsee, Manfred

2015-04-01

The Big Data era has begun also in the climate sciences, not only in economics or molecular biology. We measure climate at increasing spatial resolution by means of satellites and look farther back in time at increasing temporal resolution by means of natural archives and proxy data. We use powerful supercomputers to run climate models. The model output of the calculations made for the IPCC's Fifth Assessment Report amounts to ~650 TB. The 'scientific evolution' of grid computing has started, and the 'scientific revolution' of quantum computing is being prepared. This will increase computing power, and data amount, by several orders of magnitude in the future. However, more data does not automatically mean more knowledge. We need statisticians, who are at the core of transforming data into knowledge. Statisticians notably also explore the limits of our knowledge (uncertainties, that is, confidence intervals and P-values). Mudelsee (2014 Climate Time Series Analysis: Classical Statistical and Bootstrap Methods. Second edition. Springer, Cham, xxxii + 454 pp.) coined the term 'optimal estimation'. Consider the hyperspace of climate estimation. It has many, but not infinite, dimensions. It consists of the three subspaces Monte Carlo design, method and measure. The Monte Carlo design describes the data generating process. The method subspace describes the estimation and confidence interval construction. The measure subspace describes how to detect the optimal estimation method for the Monte Carlo experiment. The envisaged large increase in computing power may bring the following idea of optimal climate estimation into existence. Given a data sample, some prior information (e.g. measurement standard errors) and a set of questions (parameters to be estimated), the first task is simple: perform an initial estimation on basis of existing knowledge and experience with such types of estimation problems. The second task requires the computing power: explore the hyperspace to

MOLECULAR DYNAMICS COMPUTER SIMULATIONS OF MULTIDRUG RND EFFLUX PUMPS

Directory of Open Access Journals (Sweden)

Paolo Ruggerone

2013-02-01

Full Text Available Over-expression of multidrug efflux pumps of the Resistance Nodulation Division (RND protein super family counts among the main causes for microbial resistance against pharmaceuticals. Understanding the molecular basis of this process is one of the major challenges of modern biomedical research, involving a broad range of experimental and computational techniques. Here we review the current state of RND transporter investigation employing molecular dynamics simulations providing conformational samples of transporter components to obtain insights into the functional mechanism underlying efflux pump-mediated antibiotics resistance in Escherichia coli and Pseudomonas aeruginosa.

Molecular Dynamics Computer Simulations of Multidrug RND Efflux Pumps

Directory of Open Access Journals (Sweden)

Paolo Ruggerone

2013-02-01

Full Text Available Over-expression of multidrug efflux pumps of the Resistance Nodulation Division (RND protein super family counts among the main causes for microbial resistance against pharmaceuticals. Understanding the molecular basis of this process is one of the major challenges of modern biomedical research, involving a broad range of experimental and computational techniques. Here we review the current state of RND transporter investigation employing molecular dynamics simulations providing conformational samples of transporter components to obtain insights into the functional mechanism underlying efflux pump-mediated antibiotics resistance in Escherichia coli and Pseudomonas aeruginosa.

A decade of molecular cell biology: achievements and challenges.

Science.gov (United States)

Akhtar, Asifa; Fuchs, Elaine; Mitchison, Tim; Shaw, Reuben J; St Johnston, Daniel; Strasser, Andreas; Taylor, Susan; Walczak, Claire; Zerial, Marino

2011-09-23

Nature Reviews Molecular Cell Biology celebrated its 10-year anniversary during this past year with a series of specially commissioned articles. To complement this, here we have asked researchers from across the field for their insights into how molecular cell biology research has evolved during this past decade, the key concepts that have emerged and the most promising interfaces that have developed. Their comments highlight the broad impact that particular advances have had, some of the basic understanding that we still require, and the collaborative approaches that will be essential for driving the field forward.

Lattice dynamics and molecular dynamics simulation of complex materials

International Nuclear Information System (INIS)

Chaplot, S.L.

1997-01-01

In this article we briefly review the lattice dynamics and molecular dynamics simulation techniques, as used for complex ionic and molecular solids, and demonstrate a number of applications through examples of our work. These computational studies, along with experiments, have provided microscopic insight into the structure and dynamics, phase transitions and thermodynamical properties of a variety of materials including fullerene, high temperature superconducting oxides and geological minerals as a function of pressure and temperature. The computational techniques also allow the study of the structures and dynamics associated with disorder, defects, surfaces, interfaces etc. (author)

Reconciling collaborative action research with existing institutions: insights from Dutch and German climate knowledge programmes

NARCIS (Netherlands)

Termeer, C.J.A.M.; Buuren, van A.; Knieling, J.; Gottschick, M.

2015-01-01

Researchers and policymakers increasingly aim to set up collaborative research programmes to address the challenges of adaptation to climate change. This does not only apply for technical knowledge, but for governance knowledge also. Both the Netherlands and Germany have set up large scale

A Big Data Guide to Understanding Climate Change: The Case for Theory-Guided Data Science.

Science.gov (United States)

Faghmous, James H; Kumar, Vipin

2014-09-01

Global climate change and its impact on human life has become one of our era's greatest challenges. Despite the urgency, data science has had little impact on furthering our understanding of our planet in spite of the abundance of climate data. This is a stark contrast from other fields such as advertising or electronic commerce where big data has been a great success story. This discrepancy stems from the complex nature of climate data as well as the scientific questions climate science brings forth. This article introduces a data science audience to the challenges and opportunities to mine large climate datasets, with an emphasis on the nuanced difference between mining climate data and traditional big data approaches. We focus on data, methods, and application challenges that must be addressed in order for big data to fulfill their promise with regard to climate science applications. More importantly, we highlight research showing that solely relying on traditional big data techniques results in dubious findings, and we instead propose a theory-guided data science paradigm that uses scientific theory to constrain both the big data techniques as well as the results-interpretation process to extract accurate insight from large climate data .

Insights into the Molecular Mechanisms of Alzheimer’s and Parkinson’s Diseases with Molecular Simulations: Understanding the Roles of Artificial and Pathological Missense Mutations in Intrinsically Disordered Proteins Related to Pathology

Directory of Open Access Journals (Sweden)

Orkid Coskuner-Weber

2018-01-01

Full Text Available Amyloid-β and α-synuclein are intrinsically disordered proteins (IDPs, which are at the center of Alzheimer’s and Parkinson’s disease pathologies, respectively. These IDPs are extremely flexible and do not adopt stable structures. Furthermore, both amyloid-β and α-synuclein can form toxic oligomers, amyloid fibrils and other type of aggregates in Alzheimer’s and Parkinson’s diseases. Experimentalists face challenges in investigating the structures and thermodynamic properties of these IDPs in their monomeric and oligomeric forms due to the rapid conformational changes, fast aggregation processes and strong solvent effects. Classical molecular dynamics simulations complement experiments and provide structural information at the atomic level with dynamics without facing the same experimental limitations. Artificial missense mutations are employed experimentally and computationally for providing insights into the structure-function relationships of amyloid-β and α-synuclein in relation to the pathologies of Alzheimer’s and Parkinson’s diseases. Furthermore, there are several natural genetic variations that play a role in the pathogenesis of familial cases of Alzheimer’s and Parkinson’s diseases, which are related to specific genetic defects inherited in dominant or recessive patterns. The present review summarizes the current understanding of monomeric and oligomeric forms of amyloid-β and α-synuclein, as well as the impacts of artificial and pathological missense mutations on the structural ensembles of these IDPs using molecular dynamics simulations. We also emphasize the recent investigations on residual secondary structure formation in dynamic conformational ensembles of amyloid-β and α-synuclein, such as β-structure linked to the oligomerization and fibrillation mechanisms related to the pathologies of Alzheimer’s and Parkinson’s diseases. This information represents an important foundation for the successful and

Human Responses to Climate Variability: The Case of South Africa

Science.gov (United States)

Oppenheimer, M.; Licker, R.; Mastrorillo, M.; Bohra-Mishra, P.; Estes, L. D.; Cai, R.

2014-12-01

Climate variability has been associated with a range of societal and individual outcomes including migration, violent conflict, changes in labor productivity, and health impacts. Some of these may be direct responses to changes in mean temperature or precipitation or extreme events, such as displacement of human populations by tropical cyclones. Others may be mediated by a variety of biological, social, or ecological factors such as migration in response to long-term changes in crops yields. Research is beginning to elucidate and distinguish the many channels through which climate variability may influence human behavior (ranging from the individual to the collective, societal level) in order to better understand how to improve resilience in the face of current variability as well as future climate change. Using a variety of data sets from South Africa, we show how climate variability has influenced internal (within country) migration in recent history. We focus on South Africa as it is a country with high levels of internal migration and dramatic temperature and precipitation changes projected for the 21st century. High poverty rates and significant levels of rain-fed, smallholder agriculture leave large portions of South Africa's population base vulnerable to future climate change. In this study, we utilize two complementary statistical models - one micro-level model, driven by individual and household level survey data, and one macro-level model, driven by national census statistics. In both models, we consider the effect of climate on migration both directly (with gridded climate reanalysis data) and indirectly (with agricultural production statistics). With our historical analyses of climate variability, we gain insights into how the migration decisions of South Africans may be influenced by future climate change. We also offer perspective on the utility of micro and macro level approaches in the study of climate change and human migration.

Characteristic molecular vibrations of adenosine receptor ligands.

Science.gov (United States)

Chee, Hyun Keun; Yang, Jin-San; Joung, Je-Gun; Zhang, Byoung-Tak; Oh, S June

2015-02-13

Although the regulation of membrane receptor activation is known to be crucial for molecular signal transduction, the molecular mechanism underlying receptor activation is not fully elucidated. Here we study the physicochemical nature of membrane receptor behavior by investigating the characteristic molecular vibrations of receptor ligands using computational chemistry and informatics methods. By using information gain, t-tests, and support vector machines, we have identified highly informative features of adenosine receptor (AdoR) ligand and corresponding functional amino acid residues such as Asn (6.55) of AdoR that has informative significance and is indispensable for ligand recognition of AdoRs. These findings may provide new perspectives and insights into the fundamental mechanism of class A G protein-coupled receptor activation. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Insight into the substrate specificity change caused by the Y227H mutation of α-glucosidase III from the European honeybee (Apis mellifera through molecular dynamics simulations.

Directory of Open Access Journals (Sweden)

Pratchaya Pramoj Na Ayutthaya

Full Text Available Honey from the European honeybee, Apis mellifera, is produced by α-glucosidases (HBGases and is widely used in food, pharmaceutical, and cosmetic industries. Categorized by their substrate specificities, HBGases have three isoforms: HBGase I, II and III. Previous experimental investigations showed that wild-type HBGase III from Apis mellifera (WT preferred sucrose to maltose as a substrate, while the Y227H mutant (MT preferred maltose to sucrose. This mutant can potentially be used for malt hydrolysis because it can efficiently hydrolyze maltose. In this work, to elucidate important factors contributing to substrate specificity of this enzyme and gain insight into how the Y227H mutation causes substrate specificity change, WT and MT homology models were constructed, and sucrose/maltose was docked into active sites of the WT and MT. AMBER14 was employed to perform three independent molecular dynamics runs for these four complexes. Based on the relative binding free energies calculated by the MM-GBSA method, sucrose is better than maltose for WT binding, while maltose is better than sucrose for MT binding. These rankings support the experimentally observed substrate specificity that WT preferred sucrose to maltose as a substrate, while MT preferred maltose to sucrose, suggesting the importance of binding affinity for substrate specificity. We also found that the Y227H mutation caused changes in the proximities between the atoms necessary for sucrose/maltose hydrolysis that may affect enzyme efficiency in the hydrolysis of sucrose/maltose. Moreover, the per-residue binding free energy decomposition results show that Y227/H227 may be a key residue for preference binding of sucrose/maltose in the WT/MT active site. Our study provides important and novel insight into the binding of sucrose/maltose in the active site of Apis mellifera HBGase III and into how the Y227H mutation leads to the substrate specificity change at the molecular level. This

Practice and progress in integrated assessments of climate change

International Nuclear Information System (INIS)

Toth, F.L.

1995-01-01

This paper is intended to provide an overview of the state of the art integrated socioeconomic-biophysical assessments of climate change as presented at the IIASA workshop in October 1993. The paper seeks to tally the major improvements facilitated by integrated assessments in understanding the global warming problem and the crucial unresolved problems they currently face. The basic conclusion is that, as a result of a healthy diversity in practice, integrated assessments show significant progress in structuring the economic issues of climate change and providing the first broad insights into policy options. But, as some of the simple and traditional cases seem to be solved, more complex and difficult contingencies come to the fore. This suggests a long way to go to develop skills that will be required to address the numerous open issues. (author)

Application of genomics-assisted breeding for generation of climate resilient crops: Progress and prospects

Directory of Open Access Journals (Sweden)

Chittaranjan eKole

2015-08-01

Full Text Available Climate change affects agricultural productivity worldwide. Increased prices of food commodities are the initial indication of drastic edible yield loss, which is expected to surge further due to global warming. This situation has compelled plant scientists to develop climate change-resilient crops, which can withstand broad-spectrum stresses such as drought, heat, cold, salinity, flood and submergence, and pests along with increased productivity. Genomics appears to be a promising tool for deciphering the stress responsiveness of crop species with adaptation traits or in wild relatives towards identifying underlying genes, alleles or quantitative trait loci. Molecular breeding approaches have been proven helpful in enhancing the stress adaptation of crop plants, and recent advancement in next-generation sequencing along with high-throughput sequencing and phenotyping platforms have transformed molecular breeding to genomics-assisted breeding (GAB. In view of this, the present review elaborates the progress and prospects of GAB in improving climate change resilience in crop plants towards circumventing global food insecurity.

Denudational slope processes and slope response to global climate changes and other disturbances: insights from the Nepal Himalayas.

Science.gov (United States)

Fort, Monique

2016-04-01

Hillslope geomorphology results from a large range of denudational processes mainly controlled by relief, structure, lithology, climate, land-cover and land use. In most areas of the world, the "critical zone" concept is a good integrator of denudation that operates on a long-term scale. However, in large and high mountain areas, short-time scale factors often play a significant role in the denudational pattern, accelerating and/or delaying the transfer of denudation products and fluxes, and creating specific, spatially limited disturbances. We focus on the Nepal Himalayas, where the wide altitudinal range of bio-climatic zones and the intense geodynamic activity create a complex mosaic of landforms, as expressed by the present geomorphology of mountain slopes. On the basis of examples selected in the different Himalayan mountain belts (Siwaliks hills, middle mountains, High Himalaya), we illustrate different types of slopes and disturbances induced by active tectonics, climate extremes, and climate warming trends. Special attention is paid to recent events, such as landslide damming, triggered by either intense rainfalls (Kali Gandaki and Sun Kosi valleys) or the last April-May 2015 Gorkha seismic sequence (southern Khumbu). Lastly, references to older, larger events show that despite the highly dynamic environment, landforms caused by large magnitude disturbances may persist in the landscape in the long term.

Theoretical study of molecular vibrations in electron momentum spectroscopy experiments on furan: An analytical versus a molecular dynamical approach

International Nuclear Information System (INIS)

Morini, Filippo; Deleuze, Michael S.; Watanabe, Noboru; Takahashi, Masahiko

2015-01-01

The influence of thermally induced nuclear dynamics (molecular vibrations) in the initial electronic ground state on the valence orbital momentum profiles of furan has been theoretically investigated using two different approaches. The first of these approaches employs the principles of Born-Oppenheimer molecular dynamics, whereas the so-called harmonic analytical quantum mechanical approach resorts to an analytical decomposition of contributions arising from quantized harmonic vibrational eigenstates. In spite of their intrinsic differences, the two approaches enable consistent insights into the electron momentum distributions inferred from new measurements employing electron momentum spectroscopy and an electron impact energy of 1.2 keV. Both approaches point out in particular an appreciable influence of a few specific molecular vibrations of A 1 symmetry on the 9a 1 momentum profile, which can be unravelled from considerations on the symmetry characteristics of orbitals and their energy spacing

Polymorphic phase transitions: Macroscopic theory and molecular simulation.

Science.gov (United States)

Anwar, Jamshed; Zahn, Dirk

2017-08-01

Transformations in the solid state are of considerable interest, both for fundamental reasons and because they underpin important technological applications. The interest spans a wide spectrum of disciplines and application domains. For pharmaceuticals, a common issue is unexpected polymorphic transformation of the drug or excipient during processing or on storage, which can result in product failure. A more ambitious goal is that of exploiting the advantages of metastable polymorphs (e.g. higher solubility and dissolution rate) while ensuring their stability with respect to solid state transformation. To address these issues and to advance technology, there is an urgent need for significant insights that can only come from a detailed molecular level understanding of the involved processes. Whilst experimental approaches at best yield time- and space-averaged structural information, molecular simulation offers unprecedented, time-resolved molecular-level resolution of the processes taking place. This review aims to provide a comprehensive and critical account of state-of-the-art methods for modelling polymorph stability and transitions between solid phases. This is flanked by revisiting the associated macroscopic theoretical framework for phase transitions, including their classification, proposed molecular mechanisms, and kinetics. The simulation methods are presented in tutorial form, focusing on their application to phase transition phenomena. We describe molecular simulation studies for crystal structure prediction and polymorph screening, phase coexistence and phase diagrams, simulations of crystal-crystal transitions of various types (displacive/martensitic, reconstructive and diffusive), effects of defects, and phase stability and transitions at the nanoscale. Our selection of literature is intended to illustrate significant insights, concepts and understanding, as well as the current scope of using molecular simulations for understanding polymorphic

Molecular Dynamic Simulation Insights into the Normal State and Restoration of p53 Function

Directory of Open Access Journals (Sweden)

Jianzhong Chen

2012-08-01

Full Text Available As a tumor suppressor protein, p53 plays a crucial role in the cell cycle and in cancer prevention. Almost 50 percent of all human malignant tumors are closely related to a deletion or mutation in p53. The activity of p53 is inhibited by over-active celluar antagonists, especially by the over-expression of the negative regulators MDM2 and MDMX. Protein-protein interactions, or post-translational modifications of the C-terminal negative regulatory domain of p53, also regulate its tumor suppressor activity. Restoration of p53 function through peptide and small molecular inhibitors has become a promising strategy for novel anti-cancer drug design and development. Molecular dynamics simulations have been extensively applied to investigate the conformation changes of p53 induced by protein-protein interactions and protein-ligand interactions, including peptide and small molecular inhibitors. This review focuses on the latest MD simulation research, to provide an overview of the current understanding of interactions between p53 and its partners at an atomic level.

Molecular recognition of malachite green by hemoglobin and their specific interactions: insights from in silico docking and molecular spectroscopy.

Science.gov (United States)

Peng, Wei; Ding, Fei; Peng, Yu-Kui; Sun, Ying

2014-01-01

Malachite green is an organic compound that can be widely used as a dyestuff for various materials; it has also emerged as a controversial agent in aquaculture. Since malachite green is proven to be carcinogenic and mutagenic, it may become a hazard to public health. For this reason, it is urgently required to analyze this controversial dye in more detail. In our current research, the interaction between malachite green and hemoglobin under physiological conditions was investigated by the methods of molecular modeling, fluorescence spectroscopy, circular dichroism (CD) as well as hydrophobic ANS displacement experiments. From the molecular docking, the central cavity of hemoglobin was assigned to possess high-affinity for malachite green, this result was corroborated by time-resolved fluorescence and hydrophobic ANS probe results. The recognition mechanism was found to be of static type, or rather the hemoglobin-malachite green complex formation occurred via noncovalent interactions such as π-π interactions, hydrogen bonds and hydrophobic interactions with an association constant of 10(4) M(-1). Moreover, the results also show that the spatial structure of the biopolymer was changed in the presence of malachite green with a decrease of the α-helix and increase of the β-sheet, turn and random coil suggesting protein damage, as derived from far-UV CD and three-dimensional fluorescence. Results of this work will help to further comprehend the molecular recognition of malachite green by the receptor protein and the possible toxicological profiles of other compounds, which are the metabolites and ramifications of malachite green.

Combining analytical frameworks to assess livelihood vulnerability to climate change and analyse adaptation options.

Science.gov (United States)

Reed, M S; Podesta, G; Fazey, I; Geeson, N; Hessel, R; Hubacek, K; Letson, D; Nainggolan, D; Prell, C; Rickenbach, M G; Ritsema, C; Schwilch, G; Stringer, L C; Thomas, A D

2013-10-01

Experts working on behalf of international development organisations need better tools to assist land managers in developing countries maintain their livelihoods, as climate change puts pressure on the ecosystem services that they depend upon. However, current understanding of livelihood vulnerability to climate change is based on a fractured and disparate set of theories and methods. This review therefore combines theoretical insights from sustainable livelihoods analysis with other analytical frameworks (including the ecosystem services framework, diffusion theory, social learning, adaptive management and transitions management) to assess the vulnerability of rural livelihoods to climate change. This integrated analytical framework helps diagnose vulnerability to climate change, whilst identifying and comparing adaptation options that could reduce vulnerability, following four broad steps: i) determine likely level of exposure to climate change, and how climate change might interact with existing stresses and other future drivers of change; ii) determine the sensitivity of stocks of capital assets and flows of ecosystem services to climate change; iii) identify factors influencing decisions to develop and/or adopt different adaptation strategies, based on innovation or the use/substitution of existing assets; and iv) identify and evaluate potential trade-offs between adaptation options. The paper concludes by identifying interdisciplinary research needs for assessing the vulnerability of livelihoods to climate change.

Adaptation Insights

International Development Research Centre (IDRC) Digital Library (Canada)

research approach with smallholder farmers in Kitui,. Mwingi and Mutomo Districts of Kenya to develop location-specific, simplified climate information for their use. Linking Local and Exogenous Knowledge in. Constructing Climate Forecasts. The first step of engagement with farmers was to analyze climatic variability using ...

Functional adjustments of xylem anatomy to climatic variability: insights from long-term Ilex aquifolium tree-ring series.

Science.gov (United States)

Rita, Angelo; Cherubini, Paolo; Leonardi, Stefano; Todaro, Luigi; Borghetti, Marco

2015-08-01

The present study assessed the effects of climatic conditions on radial growth and functional anatomical traits, including ring width, vessel size, vessel frequency and derived variables, i.e., potential hydraulic conductivity and xylem vulnerability to cavitation in Ilex aquifolium L. trees using long-term tree-ring time series obtained at two climatically contrasting sites, one mesic site in Switzerland (CH) and one drought-prone site in Italy (ITA). Relationships were explored by examining different xylem traits, and point pattern analysis was applied to investigate vessel clustering. We also used generalized additive models and bootstrap correlation functions to describe temperature and precipitation effects. Results indicated modified radial growth and xylem anatomy in trees over the last century; in particular, vessel frequency increased markedly at both sites in recent years, and all xylem traits examined, with the exception of xylem cavitation vulnerability, were higher at the CH mesic compared with the ITA drought site. A significant vessel clustering was observed at the ITA site, which could contribute to an enhanced tolerance to drought-induced embolism. Flat and negative relationships between vessel size and ring width were observed, suggesting carbon was not allocated to radial growth under conditions which favored stem water conduction. Finally, in most cases results indicated that climatic conditions influenced functional anatomical traits more substantially than tree radial growth, suggesting a crucial role of functional xylem anatomy in plant acclimation to future climatic conditions. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Molecular and Microbial Mechanisms Increasing Soil C Storage Under Future Rates of Anthropogenic N Deposition

Energy Technology Data Exchange (ETDEWEB)

Zak, Donald R. [Univ. of Michigan, Ann Arbor, MI (United States)

2017-11-17

A growing body of evidence reveals that anthropogenic N deposition can reduce the microbial decay of plant detritus and increase soil C storage across a wide range of terrestrial ecosystems. This aspect of global change has the potential to constrain the accumulation of anthropogenic CO₂ in the Earth’s atmosphere, and hence slow the pace of climate warming. The molecular and microbial mechanisms underlying this biogeochemical response are not understood, and they are not a component of any coupled climate-biogeochemical model estimating ecosystem C storage, and hence, the future climate of an N-enriched Earth. Here, we report the use of genomic-enabled approaches to identify the molecular underpinnings of the microbial mechanisms leading to greater soil C storage in response to anthropogenic N deposition, thereby enabling us to better anticipate changes in soil C storage.

The Future of Climate Science (Invited)

Science.gov (United States)

Bishop, R.

2010-12-01

High Performance Computing is currently deployed in several centers for climate research, but not at the levels needed to achieve substantial success on a global basis, given the complexity of the problem. A quantum leap in capabilities will be necessary to handle next-generation climate models that integrate newly emerging sciences, high-resolution grids, and voluminous observational data from satellites and sophisticated ground devices. Dr. Bishop will discuss efforts to build an International Centre for Earth Simulation (ICES) based in Switzerland that takes an holistic systems approach, and that has the competence and resources to achieve new insights in this new decade, and is capable to globally influence public policy with respect to weather, climate, environment, disaster risk reduction and socio-economic development. On this progressively crowded and fragile planet, such a capability will be invaluable, Bishop believes, if not imperative, for our long-term survival. ICES could serve as a test-bed for large scale public and private development planning. Decision makers could ask ‘what if’ questions for major construction projects (such as China’s Three Gorges Dam), and then interactively evaluate alternative scenarios. Likewise, ICES could help uncover the possible unintended consequences of climate remediation and adaptation strategies, geo-engineering ideas, CO2 sequestration, deep sea drilling, etc. ICES would be a resource for building more resilient societies in an era of rapid climate change and frequent natural disasters (such as flooding, extreme weather events and volcanic ash clouds), and therefore of great consequence to our future well-being. It would ultimately play a major role in the education and training of policy-makers, the public, and future Earth Scientists - in conjunction with the current national and regional centers.

The 2008 California climate change assessment

Science.gov (United States)

Franco, G.

2008-12-01

In 2005, Governor Arnold Schwarzenegger signed Executive Order S-03-05, which laid the foundation for California's ambitious greenhouse gas mitigation reduction efforts. The 2020 goal is now codified in state law requiring bringing 2020 emissions to the 1990 levels. The Executive Order also mandates the preparation of biennial updates on the latest climate change science, potential impacts, and assessment of the state's efforts to manage its climate change risks through various adaptation options. In 2006, the first of these mandated scientific assessments (The Governor's Scenarios Report) was released. Based on new scientific studies conducted in the interim, the next assessment, the '2008 Governor's Scenarios Report' is currently in preparation. It has three principal goals: (1) to improve the assessment of climate changes for California and associated impacts on key physical and biological indicators; (2) to begin to translate these physical and biological impacts into sectoral economic impacts; and (3) to begin to develop and evaluate strategies for key sectors or regions for adapting to climate changes already underway. Contributors to this session will present some of this new research to the scientific community. Among the most exciting new insights are impacts assessments for the all-important water and agricultural sectors, coastal areas, public health and related air quality and environmental justice issues, the forestry and energy sectors. This presentation will give an overview of the overall effort which will result in about 35 scientific papers from different research institutions in California. All of the studies are interlinked in such a way as to produce a consistent overall assessment.

Fabrication of tunnel junction-based molecular electronics and spintronics devices

International Nuclear Information System (INIS)

Tyagi, Pawan

2012-01-01

Tunnel junction-based molecular devices (TJMDs) are highly promising for realizing futuristic electronics and spintronics devices for advanced logic and memory operations. Under this approach, ∼2.5 nm molecular device elements bridge across the ∼2-nm thick insulator of a tunnel junction along the exposed side edge(s). This paper details the efforts and insights for producing a variety of TJMDs by resolving multiple device fabrication and characterization issues. This study specifically discusses (i) compatibility between tunnel junction test bed and molecular solutions, (ii) optimization of the exposed side edge profile and insulator thickness for enhancing the probability of molecular bridging, (iii) effect of fabrication process-induced mechanical stresses, and (iv) minimizing electrical bias-induced instability after the device fabrication. This research will benefit other researchers interested in producing TJMDs efficiently. TJMD approach offers an open platform to test virtually any combination of magnetic and nonmagnetic electrodes, and promising molecules such as single molecular magnets, porphyrin, DNA, and molecular complexes.

Structure–performance characterization for carbon molecular sieve membranes using molecular scale gas probes

KAUST Repository

Rungta, Meha

2015-04-01

© 2015 Elsevier Ltd. All rights reserved. Understanding the relationship between carbon molecular sieve (CMS) pore structure and corresponding gas separation performance enables optimization for a given gas separation application. The final pyrolysis temperature and starting polymer precursor are the two critical parameters in controlling CMS performance. This study considers structure and performance changes of CMS derived from a commercially available polymer precursor at different pyrolysis temperatures. As reviewed in this paper, most traditional characterization methods based on microscopy, X-ray diffraction, spectroscopy, sorption-based pore size distribution measurements etc. provide limited information for relating separation performance to the CMS morphology and structural changes. A useful alternative approach based on different sized gases as molecular scale probes of the CMS pore structure was successfully used here in conjunction with separation data to provide critical insights into the structure-performance relationships of the engineered CMS.

Gathering storm. The human cost of climate change

International Nuclear Information System (INIS)

Cowell, O.; Chang, I.

2000-09-01

Friends of the Earth International demonstrates the urgency of action needed to halt climate change (Part 1). Personal testimonies from survivors of Hurricane Mitch, the Mozambique floods and other events give a chilling insight of what may lie ahead for more of us in the future (Part 2). Extreme high-temperature events, droughts, floods, cyclones and storm surges with knock-on effects for ecosystems, fires, pest outbreaks, human health, our settlements and food security (Part 3). Part 4 looks at which countries are blocking action on climate change and proposes solutions for the way forward. Much deeper cuts in greenhouse gas emissions, based on an equitable sharing of the atmosphere, are needed if dangerous climate change is to be averted. Global protection will only happen when all parties at the climate summit acknowledge the real risks of climate change and their own responsibilities in improving the situation through emissions reductions. Industrialised countries must make much deeper cuts in their greenhouse gas emissions of as much as 80-90% to keep climate change within acceptable limits while allowing developing countries space to develop, Friends of the Earth International calls on governments to ensure that decisions taken at the CoP-6 in The Hague, Netherlands (1) Ensure that the Kyoto Protocol results in real and permanent emissions reductions through the development of renewable energy sources and energy efficiency measures; (2) Commit industrialised countries to achieving 80% of their Kyoto objective through emissions reductions at home; and (3) Enshrine principles of equity in the framework for emission reductions in the next and future commitment periods based on an equal per capita approach and ecological limits [nl

Molecular diagnosis and immunotherapy.

Science.gov (United States)

Sastre, Joaquín; Sastre-Ibañez, Marina

2016-12-01

To describe recent insights into how molecular diagnosis can improve indication and selection of suitable allergens for specific immunotherapy and increase the safety of this therapy. As specific allergen immunotherapy targets specific allergens, identification of the disease-eliciting allergen is a prerequisite for accurate prescription of treatment. In areas of complex sensitization to aeroallergens or in cases of hymenoptera venom allergy, the use of molecular diagnosis has demonstrated that it may lead to a change in indication and selection of allergens for immunotherapy in a large proportion of patients when compared with diagnosis based on skin prick testing and/or specific IgE determination with commercial extracts. These changes in immunotherapy prescription aided by molecular diagnosis have been demonstrated to be cost-effective in some scenarios. Certain patterns of sensitization to grass or olive pollen and bee allergens may identify patients with higher risk of adverse reaction during immunotherapy. Molecular diagnosis, when used with other tools and patients' clinical records, can help clinicians better to select the most appropriate patients and allergens for specific immunotherapy and, in some cases, predict the risk of adverse reactions. The pattern of sensitization to allergens could potentially predict the efficacy of allergen immunotherapy provided that these immunotherapy products contain a sufficient amount of these allergens. Nevertheless, multiplex assay remains a third-level approach, not to be used as screening method in current practice.

Storm warning : gambling with the climate of our planet

Energy Technology Data Exchange (ETDEWEB)

Dotto, L.

1999-06-01

This book gives a clear explanation of climate phenomena such as El Nino and the greenhouse effect, and provides a background and insight into the climate conferences held in recent years in Rio and Kyoto. It documents the extreme weather events of recent years, including the ice storm of 1998, the 1997 Manitoba floods, and the 1998 Atlantic hurricane season. Such events should prompt people to give serious thought to the implications of global warming and consider the strong evidence that our climate is changing due to human interference. The book warns that global warming will increase the frequency and severity of extreme weather events such as floods, droughts, and storm. Climate change is expected to affect agriculture, water supplies, food production, and the spread of human diseases. The book provides thoughtful answers to the major questions about global warming, detailed descriptions of the multiple effects on human health and safety, and discusses the steps that need to be taken to avoid and adapt to the oncoming storm. In answer to the non-believers, the author points out the futility of demanding more and more proof. In her view, we have all the proof needed, it is time to concentrate not on proof but on risk. refs.

Satellite-derived SIF and CO2 Observations Show Coherent Responses to Interannual Climate Variations

Science.gov (United States)

Butterfield, Z.; Hogikyan, A.; Kulawik, S. S.; Keppel-Aleks, G.

2017-12-01

Gross primary production (GPP) is the single largest carbon flux in the Earth system, but its sensitivity to changes in climate is subject to significant uncertainty. Satellite measurements of solar-induced chlorophyll fluorescence (SIF) offer insight into spatial and temporal patterns in GPP at a global scale and, combined with other satellite-derived datasets, provide unprecedented opportunity to explore interactions between atmospheric CO2, GPP, and climate variability. To explore potential drivers of GPP in the Northern Hemisphere (NH), we compare monthly-averaged SIF data from the Global Ozone Monitoring Experiment 2 (GOME-2) with observed anomalies in temperature (T; CRU-TS), liquid water equivalent (LWE) from the Gravity Recovery and Climate Experiment (GRACE), and photosynthetically active radiation (PAR; CERES SYN1deg). Using observations from 2007 through 2015 for several NH regions, we calculate month-specific sensitivities of SIF to variability in T, LWE, and PAR. These sensitivities provide insight into the seasonal progression of how productivity is affected by climate variability and can be used to effectively model the observed SIF signal. In general, we find that high temperatures are beneficial to productivity in the spring, but detrimental in the summer. The influences of PAR and LWE are more heterogeneous between regions; for example, higher LWE in North American temperate forest leads to decreased springtime productivity, while exhibiting a contrasting effect in water-limited regions. Lastly, we assess the influence of variations in terrestrial productivity on atmospheric carbon using a new lower tropospheric CO2 product derived from the Greenhouse Gases Observing Satellite (GOSAT). Together, these data shed light on the drivers of interannual variability in the annual cycle of NH atmospheric CO2, and may provide improved constraints on projections of long-term carbon cycle responses to climate change.

Molecular Imaging of Inflammation in Atherosclerosis

Science.gov (United States)

Wildgruber, Moritz; Swirski, Filip K.; Zernecke, Alma

2013-01-01

Acute rupture of vulnerable plaques frequently leads to myocardial infarction and stroke. Within the last decades, several cellular and molecular players have been identified that promote atherosclerotic lesion formation, maturation and plaque rupture. It is now widely recognized that inflammation of the vessel wall and distinct leukocyte subsets are involved throughout all phases of atherosclerotic lesion development. The mechanisms that render a stable plaque unstable and prone to rupture, however, remain unknown and the identification of the vulnerable plaque remains a major challenge in cardiovascular medicine. Imaging technologies used in the clinic offer minimal information about the underlying biology and potential risk for rupture. New imaging technologies are therefore being developed, and in the preclinical setting have enabled new and dynamic insights into the vessel wall for a better understanding of this complex disease. Molecular imaging has the potential to track biological processes, such as the activity of cellular and molecular biomarkers in vivo and over time. Similarly, novel imaging technologies specifically detect effects of therapies that aim to stabilize vulnerable plaques and silence vascular inflammation. Here we will review the potential of established and new molecular imaging technologies in the setting of atherosclerosis, and discuss the cumbersome steps required for translating molecular imaging approaches into the clinic. PMID:24312156

Earth System Grid II, Turning Climate Datasets into Community Resources

Energy Technology Data Exchange (ETDEWEB)

Middleton, Don

2006-08-01

The Earth System Grid (ESG) II project, funded by the Department of Energy’s Scientific Discovery through Advanced Computing program, has transformed climate data into community resources. ESG II has accomplished this goal by creating a virtual collaborative environment that links climate centers and users around the world to models and data via a computing Grid, which is based on the Department of Energy’s supercomputing resources and the Internet. Our project’s success stems from partnerships between climate researchers and computer scientists to advance basic and applied research in the terrestrial, atmospheric, and oceanic sciences. By interfacing with other climate science projects, we have learned that commonly used methods to manage and remotely distribute data among related groups lack infrastructure and under-utilize existing technologies. Knowledge and expertise gained from ESG II have helped the climate community plan strategies to manage a rapidly growing data environment more effectively. Moreover, approaches and technologies developed under the ESG project have impacted datasimulation integration in other disciplines, such as astrophysics, molecular biology and materials science.

Micro-/nanostructured multicomponent molecular materials: design, assembly, and functionality.

Science.gov (United States)

Yan, Dongpeng

2015-03-23

Molecule-based micro-/nanomaterials have attracted considerable attention because their properties can vary greatly from the corresponding macro-sized bulk systems. Recently, the construction of multicomponent molecular solids based on crystal engineering principles has emerged as a promising alternative way to develop micro-/nanomaterials. Unlike single-component materials, the resulting multicomponent systems offer the advantages of tunable composition, and adjustable molecular arrangement, and intermolecular interactions within their solid states. The study of these materials also supplies insight into how the crystal structure, molecular components, and micro-/nanoscale effects can influence the performance of molecular materials. In this review, we describe recent advances and current directions in the assembly and applications of crystalline multicomponent micro-/nanostructures. Firstly, the design strategies for multicomponent systems based on molecular recognition and crystal engineering principles are introduced. Attention is then focused on the methods of fabrication of low-dimensional multicomponent micro-/nanostructures. Their new applications are also outlined. Finally, we briefly discuss perspectives for the further development of these molecular crystalline micro-/nanomaterials. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Interactive Molecular Graphics for Augmented Reality Using HoloLens.

Science.gov (United States)

Müller, Christoph; Krone, Michael; Huber, Markus; Biener, Verena; Herr, Dominik; Koch, Steffen; Reina, Guido; Weiskopf, Daniel; Ertl, Thomas

2018-06-13

Immersive technologies like stereo rendering, virtual reality, or augmented reality (AR) are often used in the field of molecular visualisation. Modern, comparably lightweight and affordable AR headsets like Microsoft's HoloLens open up new possibilities for immersive analytics in molecular visualisation. A crucial factor for a comprehensive analysis of molecular data in AR is the rendering speed. HoloLens, however, has limited hardware capabilities due to requirements like battery life, fanless cooling and weight. Consequently, insights from best practises for powerful desktop hardware may not be transferable. Therefore, we evaluate the capabilities of the HoloLens hardware for modern, GPU-enabled, high-quality rendering methods for the space-filling model commonly used in molecular visualisation. We also assess the scalability for large molecular data sets. Based on the results, we discuss ideas and possibilities for immersive molecular analytics. Besides more obvious benefits like the stereoscopic rendering offered by the device, this specifically includes natural user interfaces that use physical navigation instead of the traditional virtual one. Furthermore, we consider different scenarios for such an immersive system, ranging from educational use to collaborative scenarios.

Classroom climate in Serbia: The perspective of primary school teachers

Directory of Open Access Journals (Sweden)

Ševkušić Slavica

2014-01-01

Full Text Available The goal of this research is to gain insight into the classroom climate in schools in Serbia from the perspective of teachers. To realize this goal, we set up two research questions: (1 How do teachers assess the importance of certain aspects of the classroom climate and their own engagement in creating favourable climate, and (2 which factors determine the quality of classroom climate. We considered four dimensions of classroom climate: equality in communication, social relationships between students, respect for students’ feelings and the organizing group work. The sample consisted of primary school teachers in Serbia (N=1441, who completed a questionnaire made for our research needs. The results of factor analysis confirmed the initial assumption that the selected dimensions are related in terms of their belonging to the same construct and sufficiently different to be considered as selfcontained. The obtained results show that teachers in Serbia highly value the importance of all researched aspects of the classroom climate and believe that they are engaged to a large extent in creating a positive classroom climate. Also, it was shown that teachers’ gender and the teaching level are the most important determinants of classroom climate quality. Bearing in mind the limitations of the applied instrument it is concluded that the results should be considered with caution and that future research should include students’ perspective, direct class observation and qualitative methods to gain a more objective and more comprehensive understanding of the classroom climate. [Projekat Ministarstva nauke Republike Srbije, br. 179034: From encouraging initiative, cooperation and creativity in education to new roles and identities in society i br. 47008: Improving the quality and accessibility of education in modernization processes in Serbia

Molecular Concordance Between Primary Breast Cancer and Matched Metastases

DEFF Research Database (Denmark)

Krøigård, Anne Bruun; Larsen, Martin Jakob; Thomassen, Mads

2016-01-01

Clinical management of breast cancer is increasingly personalized and based on molecular profiling. Often, primary tumors are used as proxies for systemic disease at the time of recurrence. However, recent studies have revealed substantial discordances between primary tumors and metastases, both....... The purpose of this review is to illuminate the extent of cancer genome evolution through disease progression and the degree of molecular concordance between primary breast cancers and matched metastases. We present an overview of the most prominent studies investigating the expression of endocrine receptors......, transcriptomics, and genome aberrations in primary tumors and metastases. In conclusion, biopsy of metastatic lesions at recurrence of breast cancer is encouraged to provide optimal treatment of the disease. Furthermore, molecular profiling of metastatic tissue provides invaluable mechanistic insight...

Molecular and radiological diagnosis of sclerosing bone dysplasias

International Nuclear Information System (INIS)

Hul, Wim van; Vanhoenacker, Filip; Balemans, Wendy; Janssens, Katrien; Schepper, A.M. de

2001-01-01

Bone mineral density (BMD) is a quantitative trait for which the heritability of the variance is estimated to be up to 80%, based on epidemiological and twin studies. Further illustration of the involvement of genetic factors in bone homeostasis, is the existence of an extended group of genetic conditions associated with an abnormal bone density. The group of conditions with increased bone density has long been poorly studied and understood at the molecular genetic level but recently, thanks to recent developments in molecular genetics and genomics, for some of them major breakthroughs have been made. These findings will make the molecular analysis of such patients an additional tool in diagnostics and in genetic counseling. However, the initial identification of affected patients is still largely dependent upon recognition of clinical and radiological stigmata of the disease. Therefore, in this overview of sclerosing bone dysplasias, the classical clinical and radiological signs of this group of disorders will be discussed along with the new molecular insights

Molecular and radiological diagnosis of sclerosing bone dysplasias

Energy Technology Data Exchange (ETDEWEB)

Hul, Wim van E-mail: vhul@uia.ac.be; Vanhoenacker, Filip; Balemans, Wendy; Janssens, Katrien; Schepper, A.M. de

2001-12-01

Bone mineral density (BMD) is a quantitative trait for which the heritability of the variance is estimated to be up to 80%, based on epidemiological and twin studies. Further illustration of the involvement of genetic factors in bone homeostasis, is the existence of an extended group of genetic conditions associated with an abnormal bone density. The group of conditions with increased bone density has long been poorly studied and understood at the molecular genetic level but recently, thanks to recent developments in molecular genetics and genomics, for some of them major breakthroughs have been made. These findings will make the molecular analysis of such patients an additional tool in diagnostics and in genetic counseling. However, the initial identification of affected patients is still largely dependent upon recognition of clinical and radiological stigmata of the disease. Therefore, in this overview of sclerosing bone dysplasias, the classical clinical and radiological signs of this group of disorders will be discussed along with the new molecular insights.

Molecular simulation of the thermophysical properties of N-functionalized alkylimidazoles.

Science.gov (United States)

Turner, C Heath; Cooper, Alex; Zhang, Zhongtao; Shannon, Matthew S; Bara, Jason E

2012-06-07

Molecular simulations are used to probe the thermophysical properties of a series of N-functionalized alkylimidazoles, ranging from N-methylimidazole to N-heptylimidazole. These compounds have been previously synthesized, and their solvation properties have been shown to be potentially useful for CO(2) capture from industrial sources. In this work, we use first-principles calculations to fit electrostatic charges to the molecular models, which are then used to perform a series of molecular dynamics simulations. Over a range of different temperatures, we benchmark the simulated densities and heat capacities against experimental measurements. Also, we predict the Henry's constants for CO(2) absorption and probe the solvents' structures using molecular simulation techniques, such as fractional free volume analysis and void distributions. We find that our simulations are able to closely reproduce the experimental benchmarks and add additional insight into the molecular structure of these fluids, with respect to their observed solvent properties.

Implications of climate change on hydrological extremes in the Blue Nile basin: A review

Directory of Open Access Journals (Sweden)

Meron Teferi Taye

2015-09-01

New hydrological insights: The review illustrates some discrepancy among research outputs. For the historical context, this is partially related to the period and length of data analyzed and the failure to consider the influence of multi-decadal oscillations. Consequently, we show that annual cycle of Blue Nile flow has not changed in the past five decades. For the future context, discrepancy is partially attributable to the various and differing climate and hydrological models included and the downscaling techniques applied. The need to prudently consider sources of uncertainty and potential causes of bias in historical trend and climate change impact research is highlighted.

Molecular markers for genetic diversity and phylogeny research of ...

African Journals Online (AJOL)

Brazilian sheep descended from several breeds brought to the New World by Portuguese and Spanish colonists, and they have evolved and adapted to local climatic variations and acquired tolerance or resistance to many diseases. Molecular markers are widely used in analyzing genetic variability, and markers such as ...

Molecular events basic to cellular radiation response

International Nuclear Information System (INIS)

Kolodny, G.M.

The initiation and control of the division process in normal cells is studied to gain insight into changes in these regards caused by x-irradiation and neoplasia. The Primer Hypothesis for eukaryotic gene regulation proposes that small molecular weight RNA acts as primer for new RNA synthesis by hybridizing with DNA and there initiating the transcription of a new RNA chain. The experiments reported here indicate that small molecular weight RNA will induce the production of new proteins. These results are consistent with the Primer Hypothesis, and demonstrate that RNA can be taken up from the media by cells in culture and can induce in vitro the production of differentiated cell products

Molecular markers in transitional cell carcinoma of the bladder: New insights into mechanisms and prognosis

Directory of Open Access Journals (Sweden)

Behfar Ehdaie

2008-01-01

Full Text Available Urothelial carcinoma is potentially life-threatening and expensive to treat since for many patients, the diagnosis entails a lifetime of surveillance to detect recurrent disease. Advancements in technology have provided an understanding of the molecular mechanisms of carcinogenesis and defined distinct pathways in tumorigenesis and progression. At the molecular level, urothelial carcinoma is being seen as a disease with distinct pathways of carcinogenesis and progression and thus markers of these processes should be used as both diagnostics and predictors of progression and patient outcome. Herein we present a selective overview of the molecular underpinning of urothelial carcinogenesis and progression and discuss the potential for proteins involved in these processes to serve as biomarkers. The discovery of biomarkers has enabled the elucidation of targets for novel therapeutic agents to disrupt the deregulation underlying the development and progression of urothelial carcinogenesis.

Large resistance change on magnetic tunnel junction based molecular spintronics devices

Science.gov (United States)

Tyagi, Pawan; Friebe, Edward

2018-05-01

Molecular bridges covalently bonded to two ferromagnetic electrodes can transform ferromagnetic materials and produce intriguing spin transport characteristics. This paper discusses the impact of molecule induced strong coupling on the spin transport. To study molecular coupling effect the octametallic molecular cluster (OMC) was bridged between two ferromagnetic electrodes of a magnetic tunnel junction (Ta/Co/NiFe/AlOx/NiFe/Ta) along the exposed side edges. OMCs induced strong inter-ferromagnetic electrode coupling to yield drastic changes in transport properties of the magnetic tunnel junction testbed at the room temperature. These OMCs also transformed the magnetic properties of magnetic tunnel junctions. SQUID and ferromagnetic resonance studies provided insightful data to explain transport studies on the magnetic tunnel junction based molecular spintronics devices.

Insights into Monascus biology at the genetic level.

Science.gov (United States)

Shao, Yanchun; Lei, Ming; Mao, Zejing; Zhou, Youxiang; Chen, Fusheng

2014-05-01

The genus of Monascus was nominated by van Tieghem in 1884, but its fermented product-red mold rice (RMR), namely red yeast rice, has been used as folk medicines, food colorants, and fermentation starters for more than thousands of years in oriental countries. Nowadays, RMR is widely developed as food supplements around the world due to its functional compounds such as monacolin K (MK, also called lovastatin) and γ-aminobutyric acid. But the usage of RMR also incurs controversy resulting from contamination of citrinin (a kind of mycotoxin) produced by some Monascus strains. In the past decade, it has made great progress to Monascus spp. at the genetic level with the application of molecular biology techniques to restrain the citrinin production and increase the yields of MK and pigment in RMR, as well as aid Monascus classification and phylogenesis. Up to now, hundreds of papers about Monascus molecular biology (MMB) have been published in the international primary journals. However, to our knowledge, there is no MMB review issued until now. In this review, current understanding of Monascus spp. from the view of molecular biology will be covered and insights into research areas that need to be further investigated will also be discussed.

NGSS-Aligned, K-12 Climate Science Curricula, taught with citizen science and teacher-led inquiry methods

Science.gov (United States)

Zainfeld, S.

2017-12-01

Teacher-led inquiry into student learning is a promising method of formative assessment to gain insight into student achievement. NGSS-aligned K-12 Climate Science curricula taught with citizen science and teacher-led inquiry methods are described, along with results from a scientist-teacher collaboration survey.

Radiative and mechanical feedback into the molecular gas of NGC 253

NARCIS (Netherlands)

Rosenberg, M. J. F.; Kazandjian, M. V.; van der Werf, P. P.; Israel, F. P.; Meijerink, R.; Weiß, A.; Requena-Torres, M. A.; Güsten, R.

Starburst galaxies are galaxies or regions of galaxies undergoing intense periods of star formation. Understanding the heating and cooling mechanisms in these galaxies can give us insight to the driving mechanisms that fuel the starburst. Molecular emission lines play a crucial role in the cooling

A climate robust integrated modelling framework for regional impact assessment of climate change

Science.gov (United States)

Janssen, Gijs; Bakker, Alexander; van Ek, Remco; Groot, Annemarie; Kroes, Joop; Kuiper, Marijn; Schipper, Peter; van Walsum, Paul; Wamelink, Wieger; Mol, Janet

2013-04-01

Decision making towards climate proofing the water management of regional catchments can benefit greatly from the availability of a climate robust integrated modelling framework, capable of a consistent assessment of climate change impacts on the various interests present in the catchments. In the Netherlands, much effort has been devoted to developing state-of-the-art regional dynamic groundwater models with a very high spatial resolution (25x25 m2). Still, these models are not completely satisfactory to decision makers because the modelling concepts do not take into account feedbacks between meteorology, vegetation/crop growth, and hydrology. This introduces uncertainties in forecasting the effects of climate change on groundwater, surface water, agricultural yields, and development of groundwater dependent terrestrial ecosystems. These uncertainties add to the uncertainties about the predictions on climate change itself. In order to create an integrated, climate robust modelling framework, we coupled existing model codes on hydrology, agriculture and nature that are currently in use at the different research institutes in the Netherlands. The modelling framework consists of the model codes MODFLOW (groundwater flow), MetaSWAP (vadose zone), WOFOST (crop growth), SMART2-SUMO2 (soil-vegetation) and NTM3 (nature valuation). MODFLOW, MetaSWAP and WOFOST are coupled online (i.e. exchange information on time step basis). Thus, changes in meteorology and CO2-concentrations affect crop growth and feedbacks between crop growth, vadose zone water movement and groundwater recharge are accounted for. The model chain WOFOST-MetaSWAP-MODFLOW generates hydrological input for the ecological prediction model combination SMART2-SUMO2-NTM3. The modelling framework was used to support the regional water management decision making process in the 267 km2 Baakse Beek-Veengoot catchment in the east of the Netherlands. Computations were performed for regionalized 30-year climate change

Molecular environmental geochemistry

Science.gov (United States)

O'Day, Peggy A.

1999-05-01

The chemistry, mobility, and bioavailability of contaminant species in the natural environment are controlled by reactions that occur in and among solid, aqueous, and gas phases. These reactions are varied and complex, involving changes in chemical form and mass transfer among inorganic, organic, and biochemical species. The field of molecular environmental geochemistry seeks to apply spectroscopic and microscopic probes to the mechanistic understanding of environmentally relevant chemical processes, particularly those involving contaminants and Earth materials. In general, empirical geochemical models have been shown to lack uniqueness and adequate predictive capability, even in relatively simple systems. Molecular geochemical tools, when coupled with macroscopic measurements, can provide the level of chemical detail required for the credible extrapolation of contaminant reactivity and bioavailability over ranges of temperature, pressure, and composition. This review focuses on recent advances in the understanding of molecular chemistry and reaction mechanisms at mineral surfaces and mineral-fluid interfaces spurred by the application of new spectroscopies and microscopies. These methods, such as synchrotron X-ray absorption and scattering techniques, vibrational and resonance spectroscopies, and scanning probe microscopies, provide direct chemical information that can elucidate molecular mechanisms, including element speciation, ligand coordination and oxidation state, structural arrangement and crystallinity on different scales, and physical morphology and topography of surfaces. Nonvacuum techniques that allow examination of reactions in situ (i.e., with water or fluids present) and in real time provide direct links between molecular structure and reactivity and measurements of kinetic rates or thermodynamic properties. Applications of these diverse probes to laboratory model systems have provided fundamental insight into inorganic and organic reactions at

Decoding Pure Rotational Molecular Spectra for Asymmetric Molecules

Directory of Open Access Journals (Sweden)

S. A. Cooke

2013-01-01

Full Text Available Rotational spectroscopy can provide insights of unparalleled precision with respect to the wavefunctions of molecular systems that have relevance in fields as diverse as astronomy and biology. In this paper, we demonstrate how asymmetric molecular pure rotational spectra may be analyzed “pictorially” and with simple formulae. It is shown that the interpretation of such spectra relies heavily upon pattern recognition. The presentation of some common spectral line positions in near-prolate asymmetric rotational spectra provides a means by which spectral assignment, and approximate rotational constant determination, may be usefully explored. To aid in this endeavor we have created a supporting, free, web page and mobile web page.

The impact of computer science in molecular medicine: enabling high-throughput research.

Science.gov (United States)

de la Iglesia, Diana; García-Remesal, Miguel; de la Calle, Guillermo; Kulikowski, Casimir; Sanz, Ferran; Maojo, Víctor

2013-01-01

The Human Genome Project and the explosion of high-throughput data have transformed the areas of molecular and personalized medicine, which are producing a wide range of studies and experimental results and providing new insights for developing medical applications. Research in many interdisciplinary fields is resulting in data repositories and computational tools that support a wide diversity of tasks: genome sequencing, genome-wide association studies, analysis of genotype-phenotype interactions, drug toxicity and side effects assessment, prediction of protein interactions and diseases, development of computational models, biomarker discovery, and many others. The authors of the present paper have developed several inventories covering tools, initiatives and studies in different computational fields related to molecular medicine: medical informatics, bioinformatics, clinical informatics and nanoinformatics. With these inventories, created by mining the scientific literature, we have carried out several reviews of these fields, providing researchers with a useful framework to locate, discover, search and integrate resources. In this paper we present an analysis of the state-of-the-art as it relates to computational resources for molecular medicine, based on results compiled in our inventories, as well as results extracted from a systematic review of the literature and other scientific media. The present review is based on the impact of their related publications and the available data and software resources for molecular medicine. It aims to provide information that can be useful to support ongoing research and work to improve diagnostics and therapeutics based on molecular-level insights.

Biological response to climate change in the Arctic Ocean: The view from the past

Science.gov (United States)

Cronin, Thomas M.; Cronin, Matthew A.

2017-01-01

The Arctic Ocean is undergoing rapid climatic changes including higher ocean temperatures, reduced sea ice, glacier and Greenland Ice Sheet melting, greater marine productivity, and altered carbon cycling. Until recently, the relationship between climate and Arctic biological systems was poorly known, but this has changed substantially as advances in paleoclimatology, micropaleontology, vertebrate paleontology, and molecular genetics show that Arctic ecosystem history reflects global and regional climatic changes over all timescales and climate states (103–107 years). Arctic climatic extremes include 25°C hyperthermal periods during the Paleocene-Eocene (56–46 million years ago, Ma), Quaternary glacial periods when thick ice shelves and sea ice cover rendered the Arctic Ocean nearly uninhabitable, seasonally sea-ice-free interglacials and abrupt climate reversals. Climate-driven biological impacts included large changes in species diversity, primary productivity, species’ geographic range shifts into and out of the Arctic, community restructuring, and possible hybridization, but evidence is not sufficient to determine whether or when major episodes of extinction occurred.

Structural insights into microtubule doublet interactions inaxonemes

Energy Technology Data Exchange (ETDEWEB)

Downing, Kenneth H.; Sui, Haixin

2007-06-06

Coordinated sliding of microtubule doublets, driven by dynein motors, produces periodic beating of the axoneme. Recent structural studies of the axoneme have used cryo-electron tomography to reveal new details of the interactions among some of the multitude of proteins that form the axoneme and regulate its movement. Connections among the several sets of dyneins, in particular, suggest ways in which their actions may be coordinated. Study of the molecular architecture of isolated doublets has provided a structural basis for understanding the doublet's mechanical properties that are related to the bending of the axoneme, and has also offered insight into its potential role in the mechanism of dynein activity regulation.

America's Water in the 20th Century: Measures to address climate induced risk

Science.gov (United States)

Devineni, N.

2017-12-01

This work develops an understanding of water risk for USA considering linkages between water supply and competing demands. It explores how climate variability and changing water demands manifest as water deficits and how public-private management decisions determine regional water availability and drought resilience. We develop insights on regional water risks, infrastructure investments, sectoral allocation and policy modifications for America's future water sustainability. In this talk, I will focus on demonstrating how the variations in climate over the last century influenced changes in water use across the continent USA. A peak into our interactive modeling environment for future evolution of water use and supply will also be provided.

Impact modelling of water resources development and climate scenarios on Zambezi River discharge

Directory of Open Access Journals (Sweden)

Harald Kling

2014-07-01

New hydrological insights for the region: Comparisons between historical and future scenarios show that the biggest changes have already occurred. Construction of Kariba and CahoraBassa dams in the mid 1900s altered the seasonality and flow duration curves. Future irrigation development will cause decreases of a similar magnitude to those caused by current reservoir evaporation losses. The discharge is highly sensitive to small precipitation changes and the two climate models used give different signs for future precipitation change, suggestive of large uncertainty. The river basin model and database are available as anopen-online Decision Support System to facilitate impact assessments of additional climate or development scenarios.

Weather uncertainty versus climate change uncertainty in a short television weather broadcast

Science.gov (United States)

Witte, J.; Ward, B.; Maibach, E.

2011-12-01

For TV meteorologists talking about uncertainty in a two-minute forecast can be a real challenge. It can quickly open the way to viewer confusion. TV meteorologists understand the uncertainties of short term weather models and have different methods to convey the degrees of confidence to the viewing public. Visual examples are seen in the 7-day forecasts and the hurricane track forecasts. But does the public really understand a 60 percent chance of rain or the hurricane cone? Communication of climate model uncertainty is even more daunting. The viewing public can quickly switch to denial of solid science. A short review of the latest national survey of TV meteorologists by George Mason University and lessons learned from a series of climate change workshops with TV broadcasters provide valuable insights into effectively using visualizations and invoking multimedia-learning theories in weather forecasts to improve public understanding of climate change.

Systematic impact of institutional pressures on safety climate in the construction industry.

Science.gov (United States)

He, Qinghua; Dong, Shuang; Rose, Timothy; Li, Heng; Yin, Qin; Cao, Dongping

2016-08-01

This paper explores how three types of institutional pressure (i.e., coercive, mimetic and normative pressures) systematically impact on the safety climate of construction projects. These impacts are empirically tested by survey data collected from 186 questionnaires of construction companies operating in Shanghai, China. The results, obtained by partial least squares analysis, show that organizational management commitment to safety and employee involvement is positively related to all three institutional pressures, while the perception of responsibility for safety and health is significantly influenced by coercive and mimetic pressure. However, coercive and normative pressures have no significant effect on the applicability of safety rules and work practices, revealing the importance of external organizational pressures in improving project safety climate from a systematic view. The findings also provide insights into the use of institutional forces to facilitate the improvement of safety climate in the construction industry. Copyright © 2015 Elsevier Ltd. All rights reserved.

Past climate reconstruction: a tool for assessing site suitability

International Nuclear Information System (INIS)

Potter, G.

1978-01-01

Reconstructing past climatic variations can lead to a better understanding of possible future precipitation and groundwater recharge patterns. Work so far has led to several new insights into past climate variability and will provide input into the hydrologic modeling effort in progress for the Waste Management Program. Short-term reconstructions (0 to 350 y) suggest that the basin and range of the southwestern United States have the driest, least variable precipitation record. The Pacific Northwest shows higher variability and several trends lasting for more then 25 y. The Southern High Plains have even more variability, but the upper Midwest and Southwest vary most and have the highest precipitation amounts. Pollen and lake level data from the literature suggest that the Southwest was wetter during at least part of the last glacial maximum than it is today

Cellular and Molecular Pathways Leading to External Root Resorption

Science.gov (United States)

Iglesias-Linares, A.; Hartsfield, J.K.

2016-01-01

External apical root resorption during orthodontic treatment implicates specific molecular pathways that orchestrate nonphysiologic cellular activation. To date, a substantial number of in vitro and in vivo molecular, genomic, and proteomic studies have supplied data that provide new insights into root resorption. Recent mechanisms and developments reviewed here include the role of the cellular component—specifically, the balance of CD68+, iNOS+ M1- and CD68+, CD163+ M2-like macrophages associated with root resorption and root surface repair processes linked to the expression of the M1-associated proinflammatory cytokine tumor necrosis factor, inducible nitric oxide synthase, the M1 activator interferon γ, the M2 activator interleukin 4, and M2-associated anti-inflammatory interleukin 10 and arginase I. Insights into the role of mesenchymal dental pulp cells in attenuating dentin resorption in homeostasis are also reviewed. Data on recently deciphered molecular pathways are reviewed at the level of (1) clastic cell adhesion in the external apical root resorption process and the specific role of α/β integrins, osteopontin, and related extracellular matrix proteins; (2) clastic cell fusion and activation by the RANKL/RANK/OPG and ATP-P2RX7-IL1 pathways; and (3) regulatory mechanisms of root resorption repair by cementum at the proteomic and transcriptomic levels. PMID:27811065

Molecular evolution of lepidopteran silk proteins: Insights from the ghost moth, Hepialus californicus

Czech Academy of Sciences Publication Activity Database

Collin, M. A.; Mita, K.; Sehnal, František; Hayashi, C. Y.

2010-01-01

Roč. 70, č. 5 (2010), s. 519-529 ISSN 0022-2844 Grant - others:NSF(BE) DEB-0515868 Institutional research plan: CEZ:AV0Z50070508 Keywords : Lepidoptera * Trichoptera * silk Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.311, year: 2010

Molecular characterization of Fagaceae species using inter-primer binding site (iPBS) markers.

Science.gov (United States)

Coutinho, João Paulo; Carvalho, Ana; Martín, Antonio; Lima-Brito, José

2018-04-01

Retrotransposons (RTNs) contribute for genome evolution, influencing its size and structure. We investigated the utility of the RTN-based markers inter-primer binding site (iPBS) for the molecular characterization of 25 Fagaceae species from genera Castanea, Fagus and Quercus. The assessment of genetic diversity, relationships and structure, as well as taxonomic classification of Fagaceae based on molecular data is important for definition of conservation, forestry management strategies and discrimination among natural hybrids and their parents since natural hybridization may increase with the climate changes. Here, iPBS primers designed by other authors were tested alone and combined. Some of them were discriminative, revealed polymorphism within and among taxa allowing the production of a total of 150 iPBS markers. In addition, several monomorphic iPBS markers were also amplified in each taxon. The UPGMA dendrogram based on the pooled iPBS data revealed 27% of genetic similarity among species. The individuals were clustered per genus and most of the oaks per infrageneric group corroborating the adopted taxonomy. Globally, the iPBS markers demonstrated suitability for DNA fingerprinting, determination of phylogenies and taxonomic discrimination in Fagaceae, and could constitute a useful and alternative tool for germplasm characterization, and for definition of conservation strategies and forestry management. Moreover, these markers would be useful for fingerprinting natural hybrids that share morphological similarities with their parents. Since iPBS markers could also enable insights about RTNs evolution, an eventual correlation among iPBS polymorphism, variability of RTN insertions and/or genome size in Fagaceae is discussed.

Toward an integrative molecular approach to wildlife disease.

Science.gov (United States)

DeCandia, Alexandra L; Dobson, Andrew P; vonHoldt, Bridgett M

2018-01-29

Pathogens pose serious threats to human health, agricultural investment, and biodiversity conservation through the emergence of zoonoses, spillover to domestic livestock, and epizootic outbreaks. As such, wildlife managers are often tasked with mitigating the negative effects of disease. Yet, parasites form a major component of biodiversity that often persist. This is due to logistical challenges of implementing management strategies and to insufficient understanding of host-parasite dynamics. We advocate for an inclusive understanding of molecular diversity in driving parasite infection and variable host disease states in wildlife systems. More specifically, we examine the roles of genetic, epigenetic, and commensal microbial variation in disease pathogenesis. These include mechanisms underlying parasite virulence and host resistance and tolerance, and the development, regulation, and parasite subversion of immune pathways, among other processes. Case studies of devil facial tumor disease in Tasmanian devils (Sarcophilus harrisii) and chytridiomycosis in globally distributed amphibians exemplify the broad range of questions that can be addressed by examining different facets of molecular diversity. For particularly complex systems, integrative molecular analyses present a promising frontier that can provide critical insights necessary to elucidate disease dynamics operating across scales. These insights enable more accurate risk assessment, reconstruction of transmission pathways, discernment of optimal intervention strategies, and development of more effective and ecologically sound treatments that minimize damage to the host population and environment. Such measures are crucial when mitigating threats posed by wildlife disease to humans, domestic animals, and species of conservation concern. © 2018 Society for Conservation Biology.

From monsoon to marine productivity in the Arabian Sea: insights from glacial and interglacial climates

Science.gov (United States)

Le Mézo, Priscilla; Beaufort, Luc; Bopp, Laurent; Braconnot, Pascale; Kageyama, Masa

2017-07-01

The current-climate Indian monsoon is known to boost biological productivity in the Arabian Sea. This paradigm has been extensively used to reconstruct past monsoon variability from palaeo-proxies indicative of changes in surface productivity. Here, we test this paradigm by simulating changes in marine primary productivity for eight contrasted climates from the last glacial-interglacial cycle. We show that there is no straightforward correlation between boreal summer productivity of the Arabian Sea and summer monsoon strength across the different simulated climates. Locally, productivity is fuelled by nutrient supply driven by Ekman dynamics. Upward transport of nutrients is modulated by a combination of alongshore wind stress intensity, which drives coastal upwelling, and by a positive wind stress curl to the west of the jet axis resulting in upward Ekman pumping. To the east of the jet axis there is however a strong downward Ekman pumping due to a negative wind stress curl. Consequently, changes in coastal alongshore stress and/or curl depend on both the jet intensity and position. The jet position is constrained by the Indian summer monsoon pattern, which in turn is influenced by the astronomical parameters and the ice sheet cover. The astronomical parameters are indeed shown to impact wind stress intensity in the Arabian Sea through large-scale changes in the meridional gradient of upper-tropospheric temperature. However, both the astronomical parameters and the ice sheets affect the pattern of wind stress curl through the position of the sea level depression barycentre over the monsoon region (20-150° W, 30° S-60° N). The combined changes in monsoon intensity and pattern lead to some higher glacial productivity during the summer season, in agreement with some palaeo-productivity reconstructions.

Can "Ozzie" the Ostrich Prepare the Public for Better Learning about Climate Change?

Science.gov (United States)

Chen, R. F.; Lustick, D. S.; Lohmeier, J.; Lockwood, L.

2016-02-01

Climate change is one of the most pressing societal issues today, and educators are struggling with how to inform people of all ages and backgrounds about the reality and relevance of climate change. ScienceToGo.org has designed 12 posters that were placed on the Boston subways over the course of 15 months. Surveys of T-riders suggest that Ozzie the Ostrich is highly recognizable, is non-threatening, and is connecting Boston with climate change impacts and solutions. We hypothesize that our advertising campaign not only raises awareness about climate change in Boston and engages the public in thinking and talking about climate change, but also prepares them for learning more about climate change in the future. By exposing students to the 12 posters, we think that fears associated with climate change are lessened, and that students are more willing to explore a variety of media (newspaper articles, internet postings, peer-reviewed journal articles, data, and graphs) compared to students that are not exposed to the posters. Students will complete an initial survey, be exposed (or not exposed) to Ozzie posters, asked to explore a variety of media related to climate change, then surveyed again. Finally, focus groups will be conducted to gain insights on how students interact about climate change with or without exposure to Ozzie. We are interested in learning if exposure to brief, engaging, and humorous advertising messages will change the way students learn about climate change. This presentation will present initial results of this study.

Molecular Insights into the Potential Toxicological Interaction of 2-Mercaptothiazoline with the Antioxidant Enzyme—Catalase

Science.gov (United States)

Huang, Zhenxing; Huang, Ming; Mi, Chenyu; Wang, Tao; Chen, Dong; Teng, Yue

2016-01-01

2-mercaptothiazoline (2-MT) is widely used in many industrial fields, but its residue is potentially harmful to the environment. In this study, to evaluate the biological toxicity of 2-MT at protein level, the interaction between 2-MT and the pivotal antioxidant enzyme—catalase (CAT) was investigated using multiple spectroscopic techniques and molecular modeling. The results indicated that the CAT fluorescence quenching caused by 2-MT should be dominated by a static quenching mechanism through formation of a 2-MT/CAT complex. Furthermore, the identifications of the binding constant, binding forces, and the number of binding sites demonstrated that 2-MT could spontaneously interact with CAT at one binding site mainly via Van der Waals’ forces and hydrogen bonding. Based on the molecular docking simulation and conformation dynamic characterization, it was found that 2-MT could bind into the junctional region of CAT subdomains and that the binding site was close to enzyme active sites, which induced secondary structural and micro-environmental changes in CAT. The experiments on 2-MT toxicity verified that 2-MT significantly inhibited CAT activity via its molecular interaction, where 2-MT concentration and exposure time both affected the inhibitory action. Therefore, the present investigation provides useful information for understanding the toxicological mechanism of 2-MT at the molecular level. PMID:27537873

Terraforming the Planets and Climate Change Mitigation on Earth

Science.gov (United States)

Toon, O. B.

2008-12-01

Hopefully, purposeful geo-engineering of the Earth will remain a theoretical concept. Of course, we have already inadvertently changed the Earth, and over geologic history life has left an indelible imprint on our planet. We can learn about geo-engineering schemes by reference to Earth history, for example climate changes after volcanic eruptions provide important clues to using sulfates to modify the climate. The terrestrial planets and Titan offer additional insights. For instance, Mars and Venus both have carbon dioxide dominated greenhouses. Both have more than 10 times as much carbon dioxide in their atmospheres as Earth, and both absorb less sunlight than Earth, yet one is much colder than Earth and one is much hotter. These facts provide important insights into carbon dioxide greenhouses that I will review. Mars cools dramatically following planet wide dust storms, and Titan has what is referred to as an anti- greenhouse climate driven by aerosols. These data can be used to reassure us that we can model aerosol caused changes to the climate of a planet, and also provide examples of aerosols offsetting a gas-driven greenhouse effect. People have long considered whether we might make the other planets habitable. While most of the schemes considered belong in the realm of science fiction, it is possible that some schemes might be practical. Terraforming brings to mind a number of issues that are thought provoking, but not so politically charged as geo-engineering. For example: What criteria define habitability, is it enough for people to live in isolated glass enclosures, or do we need to walk freely on the planet? Different creatures have different needs. Is a planet habitable if plants can thrive in the open, or do animals also need to be free? Are the raw materials present on any planet to make it habitable? If not, can we make the materials, or do we have to import them? Is it ethical to change a planetary climate? What if there are already primitive

Numerical Modeling of Rocky Mountain Paleoglaciers - Insights into the Climate of the Last Glacial Maximum and the Subsequent Deglaciation

Science.gov (United States)

Leonard, E. M.; Laabs, B. J. C.; Plummer, M. A.

2014-12-01

Numerical modeling of paleoglaciers can yield information on the climatic conditions necessary to sustain those glaciers. In this study we apply a coupled 2-d mass/energy balance and flow model (Plummer and Phillips, 2003) to reconstruct local last glacial maximum (LLGM) glaciers and paleoclimate in ten study areas along the crest of the U.S. Rocky Mountains between 33°N and 49°N. In some of the areas, where timing of post-LLGM ice recession is constrained by surface exposure ages on either polished bedrock upvalley from the LLGM moraines or post-LLGM recessional moraines, we use the model to assess magnitudes and rates of climate change during deglaciation. The modeling reveals a complex pattern of LLGM climate. The magnitude of LLGM-to-modern climate change (temperature and/or precipitation change) was greater in both the northern (Montana) Rocky Mountains and southern (New Mexico) Rocky Mountains than in the middle (Wyoming and Colorado) Rocky Mountains. We use temperature depression estimates from global and regional climate models to infer LLGM precipitation from our glacier model results. Our results suggest a reduction of precipitation coupled with strongly depressed temperatures in the north, contrasted with strongly enhanced precipitation and much more modest temperature depression in the south. The middle Rocky Mountains of Colorado and Wyoming appear to have experienced a reduction in precipitation at the LLGM without the strong temperature depression of the northern Rocky Mountains. Preliminary work on modeling of deglaciation in the Sangre de Cristo Range in southern Colorado suggests that approximately half of the LLGM-to-modern climate change took place during the initial ~2400 years of deglaciation. If increasing temperature and changing solar insolation were the sole drivers of this initial deglaciation, then temperature would need to have risen by slightly more than 1°C/ky through this interval to account for the observed rate of ice recession.

New Biogeographic insight into Bauhinia s.l. (Leguminosae): integration from fossil records and molecular analyses.

Science.gov (United States)

Meng, Hong-Hu; Jacques, Frédéric Mb; Su, Tao; Huang, Yong-Jiang; Zhang, Shi-Tao; Ma, Hong-Jie; Zhou, Zhe-Kun

2014-08-10

Given that most species that have ever existed on earth are extinct, it stands to reason that the evolutionary history can be better understood with fossil taxa. Bauhinia is a typical genus of pantropical intercontinental disjunction among the Asian, African, and American continents. Geographic distribution patterns are better recognized when fossil records and molecular sequences are combined in the analyses. Here, we describe a new macrofossil species of Bauhinia from the Upper Miocene Xiaolongtan Formation in Wenshan County, Southeast Yunnan, China, and elucidate the biogeographic significance through the analyses of molecules and fossils. Morphometric analysis demonstrates that the leaf shapes of B. acuminata, B. championii, B. chalcophylla, B. purpurea, and B. podopetala closely resemble the leaf shapes of the new finding fossil. Phylogenetic relationships among the Bauhinia species were reconstructed using maximum parsimony and Bayesian inference, which inferred that species in Bauhinia species are well-resolved into three main groups. Divergence times were estimated by the Bayesian Markov chain Monte Carlo (MCMC) method under a relaxed clock, and inferred that the stem diversification time of Bauhinia was ca. 62.7 Ma. The Asian lineage first diverged at ca. 59.8 Ma, followed by divergence of the Africa lineage starting during the late Eocene, whereas that of the neotropical lineage starting during the middle Miocene. Hypotheses relying on vicariance or continental history to explain pantropical disjunct distributions are dismissed because they require mostly Palaeogene and older tectonic events. We suggest that Bauhinia originated in the middle Paleocene in Laurasia, probably in Asia, implying a possible Tethys Seaway origin or an "Out of Tropical Asia", and dispersal of legumes. Its present pantropical disjunction resulted from disruption of the boreotropical flora by climatic cooling after the Paleocene-Eocene Thermal Maximum (PETM). North Atlantic land

Service employees give as they get: internal service as a moderator of the service climate-service outcomes link.

Science.gov (United States)

Ehrhart, Karen Holcombe; Witt, L A; Schneider, Benjamin; Perry, Sara Jansen

2011-03-01

We lend theoretical insight to the service climate literature by exploring the joint effects of branch service climate and the internal service provided to the branch (the service received from corporate units to support external service delivery) on customer-rated service quality. We hypothesized that service climate is related to service quality most strongly when the internal service quality received is high, providing front-line employees with the capability to deliver what the service climate motivates them to do. We studied 619 employees and 1,973 customers in 36 retail branches of a bank. We aggregated employee perceptions of the internal service quality received from corporate units and the local service climate and external customer perceptions of service quality to the branch level of analysis. Findings were consistent with the hypothesis that high-quality internal service is necessary for branch service climate to yield superior external customer service quality. PsycINFO Database Record (c) 2011 APA, all rights reserved.

A comprehensive comparison of four species of Onchidiidae provides insights on the morphological and molecular adaptations of invertebrates from shallow seas to wetlands

Science.gov (United States)

Wang, Dongfeng; Li, Jie; Liu, Xin; Wu, Xin

2018-01-01

The Onchidiidae family is ideal for studying the evolution of marine invertebrate species from sea to wetland environments. However, comparative studies of Onchidiidae species are rare. A total of 40 samples were collected from four species (10 specimens per onchidiid), and their histological and molecular differences were systematically evaluated to elucidate the morphological foundations underlying the adaptations of these species. A histological analysis was performed to compare the structures of respiratory organs (gill, lung sac, dorsal skin) among onchidiids, and transcriptome sequencing of four representative onchidiids was performed to investigate the molecular mechanisms associated with their respective habitats. Twenty-six SNP markers of Onchidium reevesii revealed some DNA polymorphisms determining visible traits. Non-muscle myosin heavy chain II (NMHC II) and myosin heavy chain (MyHC), which play essential roles in amphibian developmental processes, were found to be differentially expressed in different onchidiids and tissues. The species with higher terrestrial ability and increased integrated expression of Os-MHC (NMHC II gene) and the MyHC gene, illustrating that the expression levels of these genes were associated with the evolutionary degree. This study provides a comprehensive analysis of the adaptions of a diverse and widespread group of invertebrates, the Onchidiidae. Some onchidiids can breathe well through gills and skin when under seawater, and some can breathe well through lung sacs and skin when in wetlands. A histological comparison of respiratory organs and the relative expression levels of two genes provided insights into the adaptions of onchidiids that allowed their transition from shallow seas to wetlands. This work provides a valuable reference and might encourage further study. PMID:29698429

Solid surface vs. liquid surface: nanoarchitectonics, molecular machines, and DNA origami.

Science.gov (United States)

Ariga, Katsuhiko; Mori, Taizo; Nakanishi, Waka; Hill, Jonathan P

2017-09-13

The investigation of molecules and materials at interfaces is critical for the accumulation of new scientific insights and technological advances in the chemical and physical sciences. Immobilization on solid surfaces permits the investigation of different properties of functional molecules or materials with high sensitivity and high spatial resolution. Liquid surfaces also present important media for physicochemical innovation and insight based on their great flexibility and dynamicity, rapid diffusion of molecular components for mixing and rearrangements, as well as drastic spatial variation in the prevailing dielectric environment. Therefore, a comparative discussion of the relative merits of the properties of materials when positioned at solid or liquid surfaces would be informative regarding present-to-future developments of surface-based technologies. In this perspective article, recent research examples of nanoarchitectonics, molecular machines, DNA nanotechnology, and DNA origami are compared with respect to the type of surface used, i.e. solid surfaces vs. liquid surfaces, for future perspectives of interfacial physics and chemistry.

Molecular characterization of Babesia peircei and Babesia ugwidiensis provides insight into the evolution and host specificity of avian piroplasmids

Directory of Open Access Journals (Sweden)

Michael J. Yabsley

2017-12-01

Full Text Available There are 16 recognized species of avian-infecting Babesia spp. (Piroplasmida: Babesiidae. While the classification of piroplasmids has been historically based on morphological differences, geographic isolation and presumed host and/or vector specificities, recent studies employing gene sequence analysis have provided insight into their phylogenetic relationships and host distribution and specificity. In this study, we analyzed the sequences of the 18S rRNA gene and ITS-1 and ITS-2 regions of two Babesia species from South African seabirds: Babesia peircei from African penguins (Spheniscus demersus and Babesia ugwidiensis from Bank and Cape cormorants (Phalacrocorax neglectus and P. capensis, respectively. Our results show that avian Babesia spp. are not monophyletic, with at least three distinct phylogenetic groups. B. peircei and B. ugwidiensis are closely related, and fall within the same phylogenetic group as B. ardeae (from herons Ardea cinerea, B. poelea (from boobies Sula spp. and B. uriae (from murres Uria aalge. The validity of B. peircei and B. ugwidiensis as separate species is corroborated by both morphological and genetic evidence. On the other hand, our results indicate that B. poelea might be a synonym of B. peircei, which in turn would be a host generalist that infects seabirds from multiple orders. Further studies combining morphological and molecular methods are warranted to clarify the taxonomy, phylogeny and host distribution of avian piroplasmids. Keywords: Africa, Babesia, Piroplasmida, Phalacrocoracidae, Spheniscidae, Tick-borne pathogen

Molecular Genetic Insights on Cheetah (Acinonyx jubatus) Ecology and Conservation in Namibia

OpenAIRE

Marker, Laurie L.; Wilkerson, Alison J. Pearks; Sarno, Ronald J.; Martenson, Janice; Breitenmoser-Würsten, Christian; O'Brien, Stephen J.; Johnson, Warren E.

2017-01-01

The extent and geographic patterns of molecular genetic diversity of the largest remaining free-ranging cheetah population were described in a survey of 313 individuals from throughout Namibia. Levels of relatedness, including paternity/maternity (parentage), were assessed across all individuals using 19 polymorphic microsatellite loci, and unrelated cheetahs (n = 89) from 7 regions were genotyped at 38 loci to document broad geographical patterns. There was limited differentiation among regi...

Molecular modeling of fentanyl analogs

Directory of Open Access Journals (Sweden)

LJILJANA DOSEN-MICOVIC

2004-11-01

Full Text Available Fentanyl is a highly potent and clinically widely used narcotic analgesic. A large number of its analogs have been synthesized, some of which (sufentanil and alfentanyl are also in clinical use. Theoretical studies, in recent years, afforded a better understanding of the structure-activity relationships of this class of opiates and allowed insight into the molecular mechanism of the interactions of fentanyl analogs with their receptors. An overview of the current computational techniques for modeling fentanyl analogs, their receptors and ligand-receptor interactions is presented in this paper.

The Climate Potentials and Side-Effects of Large-Scale terrestrial CO2 Removal - Insights from Quantitative Model Assessments

Science.gov (United States)

Boysen, L.; Heck, V.; Lucht, W.; Gerten, D.

2015-12-01

Terrestrial carbon dioxide removal (tCDR) through dedicated biomass plantations is considered as one climate engineering (CE) option if implemented at large-scale. While the risks and costs are supposed to be small, the effectiveness depends strongly on spatial and temporal scales of implementation. Based on simulations with a dynamic global vegetation model (LPJmL) we comprehensively assess the effectiveness, biogeochemical side-effects and tradeoffs from an earth system-analytic perspective. We analyzed systematic land-use scenarios in which all, 25%, or 10% of natural and/or agricultural areas are converted to tCDR plantations including the assumption that biomass plantations are established once the 2°C target is crossed in a business-as-usual climate change trajectory. The resulting tCDR potentials in year 2100 include the net accumulated annual biomass harvests and changes in all land carbon pools. We find that only the most spatially excessive, and thus undesirable, scenario would be capable to restore the 2° target by 2100 under continuing high emissions (with a cooling of 3.02°C). Large-scale biomass plantations covering areas between 1.1 - 4.2 Gha would produce a climate reduction potential of 0.8 - 1.4°C. tCDR plantations at smaller scales do not build up enough biomass over this considered period and the potentials to achieve global warming reductions are substantially lowered to no more than 0.5-0.6°C. Finally, we demonstrate that the (non-economic) costs for the Earth system include negative impacts on the water cycle and on ecosystems, which are already under pressure due to both land use change and climate change. Overall, tCDR may lead to a further transgression of land- and water-related planetary boundaries while not being able to set back the crossing of the planetary boundary for climate change. tCDR could still be considered in the near-future mitigation portfolio if implemented on small scales on wisely chosen areas.

Insights using the molecular model of Lipoxygenase from Finger millet (Eleusine coracana (L.)).

Science.gov (United States)

Tiwari, Apoorv; Avashthi, Himanshu; Jha, Richa; Srivastava, Ambuj; Kumar Garg, Vijay; Wasudev Ramteke, Pramod; Kumar, Anil

2016-01-01

Lipoxygenase-1 (LOX-1) protein provides defense against pests and pathogens and its presence have been positively correlated with plant resistance against pathogens. Linoleate is a known substrate of lipoxygenase and it induces necrosis leading to the accumulation of isoflavonoid phytoalexins in plant leaves. Therefore, it is of interest to study the structural features of LOX-1 from Finger millet. However, the structure ofLOX-1 from Finger millet is not yet known. A homology model of LOX-1 from Finger millet is described. Domain architecture study suggested the presence of two domains namely PLAT (Phospho Lipid Acyl Transferase) and lipoxygenase. Molecular docking models of linoleate with lipoxygenase from finger millet, rice and sorghum are reported. The features of docked models showed that finger millet have higher pathogen resistance in comparison to other cereal crops. This data is useful for the molecular cloning of fulllength LOX-1 gene for validating its role in improving plant defense against pathogen infection and for various other biological processes.

Molecular insights into the microbial formation of marine dissolved organic matter: recalcitrant or labile?

Science.gov (United States)

Koch, B. P.; Kattner, G.; Witt, M.; Passow, U.

2014-08-01

The degradation of marine dissolved organic matter (DOM) is an important control variable in the global carbon cycle. For our understanding of the kinetics of organic matter cycling in the ocean, it is crucial to achieve a mechanistic and molecular understanding of its transformation processes. A long-term microbial experiment was performed to follow the production of non-labile DOM by marine bacteria. Two different glucose concentrations and dissolved algal exudates were used as substrates. We monitored the bacterial abundance, concentrations of dissolved and particulate organic carbon (DOC, POC), nutrients, amino acids and transparent exopolymer particles (TEP) for 2 years. The molecular characterization of extracted DOM was performed by ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) after 70 days and after ∼2 years of incubation. Although glucose quickly degraded, a non-labile DOC background (5-9% of the initial DOC) was generated in the glucose incubations. Only 20% of the organic carbon from the algal exudate degraded within the 2 years of incubation. The degradation rates for the non-labile DOC background in the different treatments varied between 1 and 11 μmol DOC L-1 year-1. Transparent exopolymer particles, which are released by microorganisms, were produced during glucose degradation but decreased back to half of the maximum concentration within less than 3 weeks (degradation rate: 25 μg xanthan gum equivalents L-1 d-1) and were below detection in all treatments after 2 years. Additional glucose was added after 2 years to test whether labile substrate can promote the degradation of background DOC (co-metabolism; priming effect). A priming effect was not observed but the glucose addition led to a slight increase of background DOC. The molecular analysis demonstrated that DOM generated during glucose degradation differed appreciably from DOM transformed during the degradation of the algal exudates. Our

Theory of mind correlates with clinical insight but not cognitive insight in patients with schizophrenia.

Science.gov (United States)

Zhang, Qi; Li, Xu; Parker, Giverny J; Hong, Xiao-Hong; Wang, Yi; Lui, Simon S Y; Neumann, David L; Cheung, Eric F C; Shum, David H K; Chan, Raymond C K

2016-03-30

Research on the relationship between insight and social cognition, in particular Theory of Mind (ToM), in schizophrenia has yielded mixed findings to date. Very few studies, however, have assessed both clinical insight and cognitive insight when examining their relationships with ToM in schizophrenia. The current study thus investigated the relationship between clinical insight, cognitive insight, and ToM in a sample of 56 patients with schizophrenia and 30 healthy controls. Twenty-seven patients were classified as low in clinical insight according to their scores on the 'insight' item (G12) of the Positive and Negative Syndrome Scale (PANSS). Moreover, cognitive insight and ToM were assessed with the Beck Cognitive Insight Scale (BCIS) and the Yoni task, respectively. The results indicated that patients with poor clinical insight performed worse on tasks of second-order cognitive and affective ToM, while the ToM performance of patients with high clinical insight was equivalent to that of healthy controls. Furthermore, while clinical insight was correlated with ToM and clinical symptoms, cognitive insight did not correlate with clinical insight, ToM, or clinical symptoms. Clinical insight thus appears to be an important factor related to ToM in schizophrenia. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Comparison of Climate Preferences for Domestic and International Beach Holidays: A Case Study of Canadian Travelers

Directory of Open Access Journals (Sweden)

Michelle Rutty

2016-02-01

Full Text Available Coastal tourism is the largest segment of global leisure tourism and it is firmly linked to the destination’s natural resources—with climatic resources chief among them. Through observations and survey responses of beach users, studies have evaluated climatic resources for coastal tourism by quantifying optimal and unacceptable conditions. However, these studies have not taken into consideration that different forms of holidays (e.g., daytrips, short trips, main annual holiday, “once-in-a-lifetime” trip may have varying degrees of resilience to climatic conditions. This is the first study to explore whether ideal and unacceptable climatic conditions vary between domestic and international tourists. Using an in situ survey, Canadian beach users traveling domestically (n = 359 and internationally (n = 120 were examined. Key findings include statistically significant differences (p ≤ 0.05 between the two sample groups for every climate variable, with the international sample more resilient to a broader range of weather conditions, including a greater acceptance for warm temperatures, longer rainfall durations, higher wind speeds, and greater cloud cover. This study adds further insight into the complexities of evaluating climate for tourism, with implications for the demand response of tourists to climate change.

Hydrologic response and watershed sensitivity to climate warming in California's Sierra Nevada.

Directory of Open Access Journals (Sweden)

Sarah E Null

Full Text Available This study focuses on the differential hydrologic response of individual watersheds to climate warming within the Sierra Nevada mountain region of California. We describe climate warming models for 15 west-slope Sierra Nevada watersheds in California under unimpaired conditions using WEAP21, a weekly one-dimensional rainfall-runoff model. Incremental climate warming alternatives increase air temperature uniformly by 2 degrees, 4 degrees, and 6 degrees C, but leave other climatic variables unchanged from observed values. Results are analyzed for changes in mean annual flow, peak runoff timing, and duration of low flow conditions to highlight which watersheds are most resilient to climate warming within a region, and how individual watersheds may be affected by changes to runoff quantity and timing. Results are compared with current water resources development and ecosystem services in each watershed to gain insight into how regional climate warming may affect water supply, hydropower generation, and montane ecosystems. Overall, watersheds in the northern Sierra Nevada are most vulnerable to decreased mean annual flow, southern-central watersheds are most susceptible to runoff timing changes, and the central portion of the range is most affected by longer periods with low flow conditions. Modeling results suggest the American and Mokelumne Rivers are most vulnerable to all three metrics, and the Kern River is the most resilient, in part from the high elevations of the watershed. Our research seeks to bridge information gaps between climate change modeling and regional management planning, helping to incorporate climate change into the development of regional adaptation strategies for Sierra Nevada watersheds.

Climate change and public health. Effects and a dialogue with interested people

International Nuclear Information System (INIS)

Martens, P.; Huynen, M.

2001-01-01

In recent decades, it has become increasingly clear that the global climate is becoming warmer and that regional climates are changing. This report summarizes the results of an integrated assessment of vulnerability to climate change and adaptation options in the Netherlands carried out between July 2000 and July 2001 within the framework of the Dutch National Research Program on Global Air Pollution and Climate Change (NRP-2). The project's main aims were: - to provide an overview of scientific insights, expert judgements and stakeholders' perceptions of current and future impacts (positive and negative) of climate change for several economic sectors, human health, and natural systems in the Netherlands, considering various cross-sectoral interactions, - to develop a set of adaptation options for these sectors through a participatory process with the main stakeholders, - to perform an integrated assessment of cross-sectoral interactions of climate change impacts and adaptation options. Climate change impacts and adaptation options have been investigated for several important economic sectors (including agriculture, forestry, fisheries, industry, energy, transport, insurance and recreation and tourism), human health and natural systems (including soils, water and biodiversity issues).The results of this study are based on literature survey, a dialogue with experts and stakeholders. We are convinced that the report represents the most essential and relevant aspects of the impacts and adaptation options for climate change in the Netherlands, given the scenario setting of this study, the state of the art of current scientific knowledge, and today's expert and stakeholders' perceptions of the issues at stake. 3 refs [nl

Characterization of 1577 primary prostate cancers reveals novel biological and clinicopathologic insights into molecular subtypes.