Sample records for cicatricial retrolental fibroplasia

  1. Treatment of acute retrolental fibroplasia by cryopexy.

    Ben-Sira, I; Nissenkorn, I; Grunwald, E; YASSUR;, Y.


    Fifteen to 20% of premature infants weighing below 1500 g develop variable degrees of acute retrolental fibroplasia (RLF). Approximately 5% of those infants who develop RLF can be expected to become blind. A prospective study was carried out to evaluate if early cryopexy can prevent this blindness. The study showed that, among 533 infants admitted to the neonatal intensive care unit between July 1976 and December 1978, 55 had some degree of active RLF. In 18 eyes of 9 infants in whom the reti...

  2. Cicatricial Alopecia Overview

    ... Alopecia Find a Clinical Trial Journal Articles Cicatricial Alopecia Cicatricial Alopecia Overview PDF version of this document [PDF - 170 ... and hair is permanently lost. Who Gets Cicatricial Alopecia? Cicatricial alopecia is not contagious. It occurs worldwide ...

  3. Primary cicatricial alopecia

    Ali Karakuzu


    Cicatricial alopecia implies that follicular epithelium has been replaced by connective tissue. In other words cicatricial alopecia might include all forms of alopecia in which all hair follicles are permanently lost. In most of the cases, it is accepted that there is a permanent damage to follicular stem cell. While main target is hair follicle in primary cicatricial alopecia, in secondary cicatricial alopecia hair follicle damage is caused indirectly by a disease that is not in association ...

  4. Central centrifugal cicatricial alopecia

    Collin Blattner; Dennis C Polley; Frank Ferritto; Elston, Dirk M


    Central centrifugal cicatricial alopecia is a common cause of progressive permanent apical alopecia. This unique form of alopecia includes entities previously know as “hot comb alopecia,” “follicular degeneration syndrome,” “pseudopelade” in African Americans and “central elliptical pseudopelade” in Caucasians. The etiology appears to be multifactorial and the condition occurs in all races.

  5. Grade of Atypia in Dysplastic Nevi and Relationship with Dermal Fibroplasia

    Ahmet BABACAN; Banu LEBE


    Objective: Dysplastic nevus is described as an important factor in increasing the risk of melanoma. Many authors suggested dysplastic nevi should be graded according to architectural and cytological features. Dermal fibroplasia can be in concentric or lamellar fashion. In our study we aim to grade architectural and cytological atypia in dysplastic nevi and to assess the relationship between atypia and dermal fibroplasia.Material and Method: Ninety-three biopsies obtained from 71 patients were...

  6. Ocular cicatricial pemphigoid masquerading as chronic conjunctivitis: a case report

    DaCosta J


    Full Text Available Joanna DaCostaMoorfields Eye Hospital, London, UKAbstract: Conjunctivitis is often considered an innocuous condition which is self-limiting. This report describes misdiagnosis of ocular cicatricial pemphigoid as chronic conjunctivitis. Ocular cicatricial pemphigoid is a rare autoimmune condition. The clinical features which are useful to distinguish this condition from infective conjunctivitis are discussed. The investigation and treatment of ocular cicatricial pemphigoid is discussed. It is important to recognize nonocular symptoms and signs that may indicate the presence of a more serious underlying pathological condition necessitating specialized ophthalmic referral and subsequent investigation and treatment.Keywords: autoimmune, conjunctivitis, ocular cicatricial pemphigoid

  7. Etiology of cicatricial alopecias: a basic science point of view.

    McElwee, Kevin J


    This article presents a short summary of our current knowledge of cicatricial alopecia disease pathogenesis and the hypothetical disease mechanisms that may be involved in scarring alopecia development. Several forms of scarring alopecia likely involve targeted cytotoxic action against hair follicle cells mediated by a folliculocentric inflammation. However, the specific nature of the inflammatory interference in hair follicle growth is open to question. A popular hypothesis of lymphocyte-mediated scarring alopecia development involves autoimmune targeting of hair follicle-specific self-antigens, although there is no direct evidence in support of such a view. Alternative hypotheses focus on defects in sebaceous gland function, destruction of hair follicle stem cells, and interference in the communication between hair follicle mesenchyme and epithelium. Many questions arise from these hypotheses, and addressing them with a systematic research approach may enable significant advances in understanding cicatricial alopecia etiology. PMID:18715290

  8. Follicular unit transplantation for the treatment of secondary cicatricial alopecia

    SHAO, HUAWEI; Hang, Hu; Yunyun, Jin; Hongfei, Jiang; Chunmao, Han; Zhang, Jufang; Shen, Haiyan; Zhu, Fei; Jia, Ming; Wang, Yuyan; Guo, Xiaobo


    Cicatricial alopecia, secondary to burns, trauma, surgery or radiation, describes a diverse group of disorders characterized by follicular destruction and permanent hair loss. Although surgical treatments for the condition are available, they often result in unfavourable scarring or hair growth direction. Although not yet validated in large clinical trials, follicular unit transplantation has shown promise. The authors of this Chinese study describe their experience with the technique perform...

  9. Differentiating Central Centrifugal Cicatricial Alopecia and Androgenetic Alopecia in African American Men: Report of Three Cases

    Davis, Erica C.; Reid, Sophia D.; Callender, Valerie D.; Sperling, Leonard C.


    Central centrifugal cicatricial alopecia is a scarring alopecia that is predominantly seen in African American women, but occurs less frequently in men. The authors present three cases of African American men with biopsy-proven central centrifugal cicatricial alopecia and detail the clinical presentation, histological findings, and treatment regimens. Central centrifugal cicatricial alopecia should be considered in the differential diagnosis when evaluating male patients with vertex hair loss...

  10. Cicatricial Pemphigoid in Accompany with Rheumatoid Arthritis: a Case Report

    Di Wu; Li-ming Zhang; Ying Jiang


    @@ CICATRICIAL pemphigoid (CP, also known as benign mucous membrane pemphigoid) is a rare chronic autoimmune subepithelial blister-ing disease, with an incidence of 1 per million, characterized by erosive lesions of mucous membranes and skin that result in scarring.1,2 Rheumatoid arthritis (RA) is a symmetric inflammatory arthritis that mainly affects the small joints of hands and feet, with a prevalence of 0.3% in China. In this case report we described the diagnosis of and treatment for a patient developing CP 18 years after the onset of RA, a combination rarely en-countered or reported so far.

  11. Clinical features of primary cicatricial alopecia in Chinese patients

    Shiling Qi


    Full Text Available Background: There have been few reports on primary cicatricial alopecias (PCR especially from Asia (PCA. Aims: To study the clinical, pathological and dermoscopic characteristics of PCA among Chinese patients. Methods: A retrospective analysis of the clinical data of 59 patients with PCA was conducted and the dermoscopic, pathological, treatment and prognosis characteristics analyzed. Fisher′s Chi-square exact test, Kruskal-Wallis and Spearman rank correlation test were performed. Results: The ratio of neutrophilic to lymphocytic cicatricial alopecias was about 1.3:1 in this group. The most frequent disorder was folliculitis decalvans. Follicular openings were absent on dermoscopy in all cases except alopecia mucinosa. Patulous follicular openings were characterisitc of alopecia mucinosa. After treatment, an increase in short vellus hairs was the earliest feature, while telangiectasia, epidermal scale, follicular hyperkeratosis, pustules and hair diameter diversity gradually decreased or even disappeared. Improvement in the areas of hair loss after treatment was seen more often in discoid lupus erythematosus, folliculitis decalvans and dissecting cellulitis than in patients with classic pseudopelade of Brocq. Nine patients (13.6% relapsed after cessation of therapy. Female patients needed longer treatment times. Long duration, large areas of hair loss and shorter treatment courses were the major factors in relapses. Conclusions: Dermatoscopy provides a rapid, practical and useful aid for the diagnosis of PCA and also to assess disease activity. Patulous follicular openings are a specific dermoscopic sign of alopecia mucinosa. Lichen planopilaris is less common in China than in the West.

  12. Is seborrhoeic dermatitis associated with a diffuse, low-grade folliculitis and progressive cicatricial alopecia?

    Pitney, Lucy; Weedon, David; Pitney, Michael


    An association between adult scalp seborrhoeic dermatitis and cicatricial hair loss has not previously been convincingly established. This study seeks to demonstrate a unique relationship between a clinically identifiable chronic scalp dermatitis-folliculitis with the characteristic histological features of low-grade inflammatory fibrosing alopecia, resulting in a distinctive progressive cicatricial alopecia which we believe is prevalent and hitherto unrecognised, and befits the description of seborrhoeic folliculitis. The clinical, epidemiological and histopathological features of seborrhoeic folliculitis are demonstrated to establish its unique status among the disorders of adult diffuse cicatricial alopecia. PMID:25753934

  13. Clinical assessment of frequency and structure of subepithelial fibroplasias of a cornea after a photorefractive keratectomy in patients with refraction anomalies

    V. V. Egorov


    Full Text Available Purpose: To investigate the frequency and structure of a subepithelial fibroplasia of a cornea after photorefractive keratectomy concerning myopia correction.Methods: 170 patients (337 eyes with the following refraction anomalies: stationary myopia from 1.5 to 13.0 D with astigmatism from 0.75 to 4.25 D. Frequency of survey in the postoperative period — in 1, 3, 6, 12 months. To all patients Dexamethasone instillation according to the decreasing scheme was carried out. Existence of biomicroscopic signs of any degrees of subepithelial fibroplasias of a cornea, existence and extent of loss of uncorrected visual acuity in comparison with initial, regress of refractive effect of operation were taped. Subjective estimation of quality of vision was made by patients in 1 year after operations.Results: Subepithelial fibroplasias with intensity 1.0‑2.5 points stopped in whole or in part after additional treatment, were surveyed as clinically significant and made 4,75 % (16 eyes, 9 patients. Terms of their emergence: from 1 to 10 months after operation. At 3 patients (6 eyes intensity of fibroplasias made 2.0‑2.5 points before treatment and 1.0‑2.5 points after it. To the observation extremity 7 patients from this group had visual complaints, a discomfort in an eye. Indicators of Subjective estimation of quality of vision in patients with clinically significant fibroplasias of a cornea varied from 5 to 2.9 points.Conclusion: Frequency of clinically significant fibroplasias after photorefractive keratectomy made 4.75 %. After treatment theirintensity made 1.5‑2.5 points only in 4 eyes (2 patients.

  14. Central centrifugal cicatricial alopecia amalgamated with alopecia areata: immunologic findings

    Ana Maria Abreu Velez


    Full Text Available Introduction: Both scarring and non-scarring alopecias exist; however, rare cases demonstrate features of both classes. Case Report: We describe an interesting alopecia case with amalgamated clinical, histologic and immunopathologic features of scarring and non-scarring alopecia. Specifically, the case displays combined features of alopecia areata (AA and of central centrifugal cicatricial alopecia (CCCA. A 36 year old female presented with symmetric, round, patchy hair loss on her scalp. Methods: Biopsies for hematoxylin and eosin (H&E examination, as well as for special stains, direct immunofluorescence (DIF and immunohistochemistry (IHC were performed. Results: The H&E biopsy revealed focally diminished hair follicular units, and sebaceous gland damage. Perifollicular concentric fibrosis was confirmed by Verhoeff elastin special staining. Antibodies to micelles were noted. Positive IHC staining for CD4, CD8, CD45 and multiple proteases and protease inhibitors was noted around selected follicular unit remnants. Conclusion: We present a rare alopecia, combining histologic features of CCCA with additional, selected immunologic features of AA.

  15. Primary cicatricial alopecias: a review of histopathologic findings in 38 patients from a clinical University Hospital in Sao Paulo, Brazil

    Emanuella Rosyane Duarte Moure


    Full Text Available BACKGROUND: Scarring alopecias are classified into primary and secondary types according to the initial site of inflammation. In primary scarring alopecias, the hair follicle is the main target of destruction; the term secondary cicatricial alopecia implies that follicular destruction is not the primary pathologic event. AIMS: To review the histopathologic diagnoses of cases of cicatricial alopecia in order to classify them according to the North American Hair Research Society. PATIENTS AND METHODS: Patients with biopsy specimens diagnosed as cicatricial alopecia seen from 2000 to 2005 at the Dermatologic Department of Hospital das Clinicas, São Paulo University Medical School had hematoxylin and eosin, Periodic acid-Schiff and Weigert stained slides reevaluated and sub-typed into different primary cicatricial alopecias. RESULTS: Thirty-eight cases of primary cicatricial alopecias were reclassified as: chronic cutaneous lupus (17, lichen planus pilaris (4, pseudopelade of Brocq (12, folliculitis decalvans (3, dissecting folliculitis (1, and non-specific scarring alopecia (1. In our cases, the methods employed allowed an accurate diagnosis in 12 of 13 cases (92.3% previously classified as non-specific cicatricial alopecias. CONCLUSIONS: Even in the late, pauci or non-inflammatory phases, an approach with systematic evaluation of a constellation of criteria in routine hematoxylin and eosin stain, Periodic acid-Schiff and Weigert stain allowed for a more accurate diagnosis of cicatricial alopecias.

  16. Esophageal Cicatricial Pemphigoid as an Isolated Involvement Treated with Mycophenolate Mofetil

    Sandra Sánchez Prudencio


    Full Text Available Cicatricial pemphigoid (CP is a rare blistering autoimmune disease. Esophageal involvement occurs in widespread disease and rarely appears as the only affected organ. We report a 67-year-old Caucasian female with esophageal dysphagia and weight loss. Several oral panendoscopies showed multiple exudative ulcerations with fibrin and webs in mid- and proximal esophagus and a peeling mucosa. There were no lesions in other organs. We established the diagnosis performing a direct immunofluorescence (DIF, demonstrating IgG3 and complement deposition along the basement membrane. As initial treatment the patient received prednisone 60 mg and 1 gr twice daily of mycophenolate mofetil (MMF as a steroid-sparing agent due to its lower toxicity and its selective mechanism of action. Six months later there was a significant clinical improvement and the esophageal ulcerations had disappeared, developing cicatricial fibrous rings, although no stenosis was present. Four years later, the patient remains asymptomatic with a low maintenance dose of MMF.

  17. An epidemiological study of 97 cases of primary cicatricial alopecia in Iran

    Akram Beheshtiroy


    Full Text Available Cicatricial alopecia can produce major psychosocial disturbance. This study aimed to evaluate epidemiological features of primary cicatricial alopecia (PCA. Epidemiological and histological types of scarring alopecia were determined. The chi-squared test and t-test were performed to examine the relationship between different variables using SPSS 16. A total of 97 cases, 35 (36.1% men and 62 (63.9% women, with an average age of 37 (SD=12.7, were included in this study. The female/male (F/M ratio was 1.7:1 for total cases, 1.92:1 for the lymphocytic type, and 1:1.5 for the neutrophilic type. No relationship was found between type of job (indoor or outdoor, clinical findings and histological types. Discoid lupus erythematous (DLE was the most common histological type (63.9%. PCA was found to mainly affect middle-aged individuals. The high percentage of DLE in the present study is in agreement with some similar studies.

  18. TGF-β1 induces an age-dependent inflammation of nerve ganglia and fibroplasia in the prostate gland stroma of a novel transgenic mouse.

    David A Barron

    Full Text Available TGF-β1 is overexpressed in wound repair and in most proliferative disorders including benign prostatic hyperplasia and prostate cancer. The stromal microenvironment at these sites is reactive and typified by altered phenotype, matrix deposition, inflammatory responses, and alterations in nerve density and biology. TGF-β1 is known to modulate several stromal responses; however there are few transgenic models to study its integrated biology. To address the actions of TGF-β1 in prostate disorders, we targeted expression of an epitope tagged and constitutively active TGF-β1 via the enhanced probasin promoter to the murine prostate gland epithelium. Transgenic mice developed age-dependent lesions leading to severe, yet focal attenuation of epithelium, and a discontinuous basal lamina. These changes were associated with elevated fibroplasia and frequency of collagenous micronodules in collapsed acini, along with an induced inflammation in nerve ganglia and small vessels. Elevated recruitment of CD115+ myeloid cells but not mature macrophages was observed in nerve ganglia, also in an age-dependent manner. Similar phenotypic changes were observed using a human prostate epithelium tissue recombination xenograft model, where epithelial cells engineered to overexpress TGF-β1 induced fibrosis and altered matrix deposition concurrent with inflammation in the stromal compartment. Together, these data suggest that elevated TGF-β1 expression induces a fibroplasia stromal response associated with breach of epithelial wall structure and inflammatory involvement of nerve ganglia and vessels. The novel findings of ganglia and vessel inflammation associated with formation of collagenous micronodules in collapsed acini is important as each of these are observed in human prostate carcinoma and may play a role in disease progression.

  19. Cicatricial Pemphigoid

    ... several ways to treat pemphigoid, including tetracycline with niacinamide, cortisone-type drugs that suppress the immune reaction, ... Another alternative is the use of tetracycline and niacinamide, taking one capsule of each, four times daily. ...

  20. Association of DQw7 (DQB1*0301) with ocular cicatricial pemphigoid.

    Ahmed, A R; Foster, S; Zaltas, M; Notani, G; Awdeh, Z; Alper, C A; Yunis, E J


    Ocular cicatricial pemphigoid (OCP) is an autoimmune blistering disease that affects the conjunctiva and multiple mucous membranes. Class I and II and complement genetic markers of the major histocompatibility complex were studied in 20 Caucasian OCP patients and members of their families. Frequencies of individual alleles and common fixed or extended haplotypes in the patients were compared with those in normal family control haplotypes and with overall normal Caucasian haplotypes. The most striking increase compared with overall controls was noted in HLA-DQw3 (P = 0.006), unassociated with any extended haplotype. All but 1 of the 20 patients carried DQw3 in linkage with HLA-DR4 (increased significantly with P = 0.042 compared with overall normal genotype controls) or DR5. The DQw3, on analysis by restriction fragment length polymorphism in genomic DNA, was, in every instance, DQw7 (3.1, DQB1*0301). The frequency of DQB1*0301 in patient haplotypes compared with overall normal DR4 and DR5 DQw3-bearing haplotypes was statistically significantly increased (P less than 0.003, relative risk = 9.6). The distribution of homozygotes and heterozygotes for DQB1*0301 among the patients was consistent with dominant but not recessive inheritance of DQB1*0301 or a gene, probably a class II allele, in linkage disequilibrium with it as the major histocompatibility complex susceptibility gene for OCP. Images PMID:1763074

  1. Upper lid crease approach for margin rotation in trachomatous cicatricial entropion without external sutures

    Antonio Augusto Velasco e Cruz


    Full Text Available ABSTRACT Purpose: To describe the use of a lid crease incision for upper eyelid margin rotation in cicatricial entropion combining internal traction on the anterior lamella, tarsotomy, and tarsal overlap without external sutures. Methods: Surgical description: The main steps of the procedure consisted of exposure of the entire tarsal plate up to the eyelashes followed by tarsotomy through the conjunctiva. A double-armed 6.0 polyglactin suture was then passed through the distal tarsal fragment to the marginal section of the orbicularis oculi muscle. As the sutures were tied, the distal tarsus advanced over the marginal section, and traction was exerted on the marginal strip of the orbicularis muscle. There were no bolsters or external knots. The pretarsal skin-muscle flap was closed with a 6.0 plain gut suture. Results: We used this procedure at a tertiary hospital in Saudi Arabia from 2013 to 2014. Sixty upper lids of 40 patients (23 women and 17 men were operated on, with an age range of 44-99 years [mean ± standard deviation (SD = 70.9 ± 13.01 years]. Bilateral surgery was performed on 21 patients. Follow-up ranged from 1 to 12 months (mean 3.0 ± 2.71 months. Forty percent of the patients (24 lids had more than 3 months' follow-up. The postoperative lid margin position was good in all cases. Trichiasis (two lashes was observed in only one patient with unilateral entropion on the medial aspect of the operated lid. Conclusions: The upper lid margin can be effectively rotated through a lid crease incision with internal sutures. The technique combines the main mechanisms of the Wies and Trabut approaches and avoids the use of bolsters or external sutures, which require a second consultation to be removed. Some other lid problems, such as ptosis, retraction, or dermatochalasis, can be concomitantly addressed during the procedure.

  2. Imunofluorescência direta no diagnóstico de casos suspeitos de penfigóide cicatricial ocular

    Donato Waleska Belmino Chaves


    Full Text Available OBJETIVOS: O objetivo deste estudo é caracterizar por meio do exame oftalmológico os pacientes com suspeita clínica de penfigóide cicatricial ocular e apresentar os resultados obtidos com a técnica de imunofluorescência direta. MÉTODOS: Estudo prospectivo realizado no setor de Córnea e Doenças Externas da Universidade Federal de São Paulo. Foram examinados 18 pacientes com suspeita clínica de penfigóide cicatricial ocular e realizadas biópsias de conjuntiva para o processamento da imunofluorescência direta em 13 pacientes (26 olhos, que não estavam na vigência de imunossupressão sistêmica ou terapêutica antiglaucomatosa tópica. RESULTADOS: Segundo o estadiamento de Foster, a proporção de olhos classificados de I a IV foram: 3,8%, 3,8%, 77%, 15,4% respectivamente. Apenas 3 (23% pacientes apresentaram imunofluorescência direta positiva. CONCLUSÃO: A maioria dos pacientes encontravam-se em estágio avançado da doença, o que denota retardo no diagnóstico. A imunofluorescência direta apresentou baixa sensibilidade em detectar depósitos de imunocomplexos na membrana basal da conjuntiva, quando comparada aos dados da literatura.

  3. Cicatricial ectropion correction in a patient with pyoderma gangrenosum: case report Correção de ectrópio cicatricial em paciente com pioderma gangrenoso: relato de caso

    Fernando Procianoy


    Full Text Available A 19 year old female with pyoderma gangrenosum was referred to the oculoplastic clinic for evaluation of severe cicatricial ectropion of the right lower eyelid. Examination evidenced important scarring of lower eyelid and malar region with secondary ectropion. A full-thickness skin graft associated with a lateral tarsal strip procedure and scar tissue release was performed. The patient's corticosteroid dose was raised for the procedure, and after surgery there was no healing problem or ulcer formation in the eyelid or in the graft donor site. As pyoderma gangrenosum is associated with a pathergy phenomenon in up to 25% of the cases, the onset of new lesions is a relevant concern when performing surgery in these patients. In this case, the surgery was safely performed under corticosteroid immunosuppression.Paciente do sexo feminino de 19 anos com pioderma gangrenoso foi encaminhada ao setor de Plástica Ocular para avaliação de ectrópio cicatricial grave da pálpebra inferior direita. O exame evidenciou cicatrização importante na pálpebra inferior e região malar com um ectrópio secundário. Foi realizado liberação do tecido cicatricial e um enxerto de pele total associado a "tarsal strip". A dose de corticóide da paciente foi aumentada para a realização do procedimento e não houve problemas de cicatrização ou formação de úlceras na pálpebra ou no sítio doador após a cirurgia. Como o pioderma gangrenoso é associado ao fenômeno de patergia em até 25% dos casos, o surgimento de novas lesões é uma preocupação relevante ao indicar cirurgia nestes pacientes. Neste caso, a cirurgia foi realizada com segurança sob imunossupressão com corticóide.

  4. The Treatment experience of Cicatricial eyelid ectropion after severe burns%严重烧伤后瘢痕性睑外翻的治疗体会

    李丽; 牛惠彩; 李卫卫; 王超


    Objective:To investigate rational methods to repair different type of cicatricial ectropion .Methods:20 patients with different type and degree of cicatricial ectropion were repaired with the method of simple skin grafting,nasolabial groove skin flap pedicled with inner canthus vessels,posterior auricular refluxing axial skin flap or musculus dormitator skin flap.Results:After 3-6 months follow—up observation all patients got good repaired effects.Necrobiosis occurred in distal skin flap of one patient with posterior auricular refluxing axial skin flap method.Conclusion:Cicatricial ectropion was complicated.Appropriate method should be applied to different cicatricial ectropion to achieve good results.%目的:探讨面部严重烧伤后导致不同程度瘢痕性睑外翻的修复方法。方法:对20例烧伤后不同程度的瘢痕性睑外翻,采用内眦血管蒂鼻唇沟皮瓣、眼轮匝肌皮瓣修复及瘢痕松解植皮修复。结果:皮片局部皮瓣均一期成活,1例返流轴型耳后岛状皮瓣术后皮瓣远端出现表皮坏死,经换药后创面愈合。随访3~10月,眼睑外翻均得到充分矫正,效果良好。结论:瘢痕性睑外翻病情复杂多样,须根据具体情况采用恰当方法,可取得良好效果。

  5. Community-based transconjunctival marginal rotation for cicatricial trachoma in Indians from the Upper Rio Negro basin

    O.E. Soares


    Full Text Available The objective of the present study was to describe, for the first time in Brazil, the use by a non-ophthalmologist of a community-based marginal rotation procedure by a posterior approach in the indigenous population from the Upper Rio Negro basin. Seventy-three upper eyelids of 46 Indians (11 males and 35 females with cicatricial upper eyelid entropion and trichiasis were operated in the Indian communities using a marginal rotational procedure by a posterior approach by a non-ophthalmologist physician who had general surgery experience but only an extremely short period (one week of ophthalmic training. Subjects were reevaluated 6 months after surgery. Results were classified according to the presence and location of residual trichiasis and symptoms were assessed according to a three-level subjective scale (better, worse or no change. Fifty-six eyelids (76.7% were free from trichiasis, whereas residual trichiasis was observed in 17 eyelids (23.3% of 10 subjects. In these cases, trichiasis was either lateral or medial to the central portion of the lid. Of these 10 patients, only 4 reported that the surgery did not improve the irritative symptoms. We conclude that marginal rotation by a posterior approach is an effective and simple procedure with few complications, even when performed by non-specialists. Due to its simplicity the posterior approach is an excellent option for community-based upper eyelid entropion surgery.

  6. Atualização no tratamento das ceratoconjuntivites cicatriciais Update of the treatment of cicatricial keratoconjunctivitis

    José Alvaro Pereira Gomes


    Full Text Available As ceratoconjuntivites cicatriciais (CCC representam um grupo de doenças que induz seis tipos principais de alterações oculares: olho seco; alterações palpebrais; destruição do limbo e células germinativas corneais; destruição da membrana basal; processo inflamatório; alteração na integração neuroanatômica da superfície ocular. Essas alterações acabam causando instabilidade epitelial corneal, vascularização e inflamação crônica. O resultado final é a perda de transparência da córnea e diminuição da acuidade visual. O autor descreve os seis tipos de alterações e faz uma revisão atualizada do tratamento de cada um deles.Cicatricial keratoconjunctivitis is a group of diseases that induces six different types of ocular disorders: dry eye; eyelid blinking disturbances; destruction of limbal stem cells; destruction of basement membrane; inflammation; and neuroanatomic disintegration. These disorders cause corneal epithelial instability, neovascularization and chronic inflammation which result in loss of corneal transparency and decreased visual acuity. The author describes the six types of disorders and reviews the latest therapeutic approaches for each of them.

  7. Clinicopathological analysis of 53 cases of cicatricial alopecia%53例瘢痕性秃发的临床和病理特点分析

    戚世玲; 赵莹; 张小婷; 张斌; 杨建; 章星琪


    目的 探讨瘢痕性脱发的临床、组织病理与皮肤镜特点,以及治疗和预后的特征.方法 回顾性分析53例瘢痕性脱发患者的临床资料,分析其组织病理、皮肤镜征象、治疗和预后的特点.结果 瘢痕性脱发以脱发、毛囊开口消失、毛囊皮脂腺单位数目减少或消失为共同特征.基底细胞局灶液化变性、毛囊角栓、毛细血管分支状扩张、免疫荧光阳性提示盘状红斑狼疮;界面皮炎提示毛发扁平苔藓;炎症和弹力纤维轻微破坏提示假性斑秃;毛囊间黏蛋白沉积及毛囊口下陷为黏蛋白性脱发的特点;脓疱可见于脱发性毛囊炎和分割性蜂窝织炎或毛囊炎,前者多伴有簇状发,窦道形成则仅见于后者.治疗对淋巴细胞性患者使用免疫抑制剂,对中性粒细胞性患者使用抗生素和维A酸.结论 组织病理检查对诊断起决定性作用.瘢痕性脱发造成不可逆的毛囊损害,治疗周期长.早期诊断及早期合理用药可控制疾病发展,减少永久性脱发损害.%Objective To investigate the clinical,histopathological and dermoscopic features as well as treatment and prognosis of cicatricial alopecia.Methods Clinical data on 53 patients with cicatricial alopecia were retrospectively collected and studied.Pathological and dermoscopic characteristics,as well as treatment modality and prognosis of cicatricial alopecia were analyzed.Results Cicatricial alopecia was characterized by alopecia,disappearance of follicular ostia,and absence or decrease in the number of polisebaceous gland units.Pathologically,focal liquefactive degeneration of basal cells,follicular keratotic plugs,arborising telangiectasia together with a positive immunofluorescence test were usually suggestive of discoid lupus erythematosus,interface dermatitis suggestive of lichen planopilaris,inflammation and mild disruption of elastic fibers suggestive of classic pseudopelade of Brocq.Mucin deposition between hair

  8. Membrana amniótica nas cirurgias reconstrutivas da superfície ocular nas ceratoconjuntivites cicatriciais Amniotic membrane for ocular surface reconstruction in cicatricial keratoconjunctivitis

    José Alvaro Pereira Gomes; Ciro M. Komagome; Namir Santos; Alessandra P. Chaves; Marcelo C. Cunha; Denise de Freitas


    Objetivo: A membrana amniótica tem se consolidado como útil adjuvante no tratamento de afecções da superfície ocular. Sua utilização baseia-se na capacidade de beneficiar o processo de epitelização, além de reduzir os processos inflamatório, angiogênico e cicatricial. O objetivo deste trabalho foi investigar a utilização da membrana amniótica como adjuvante no tratamento das ceratoconjuntivites cicatriciais. Métodos: A membrana amniótica foi captada a partir de parto cesárea e conservada em m...

  9. Estudo morfométrico e morfológico da cicatrização após uso do laser erbium: YAG em tecidos cutâneos de ratos Morphometrical and morphological study of cicatricial effects caused by application of laser erbium: YAG on the skin of rats

    Lúcia de Noronha


    Full Text Available Este trabalho analisa o efeito da reparação cutânea do laser erbium:yttrium aluminunn garnet (YAG (Er:YAG em pele de ratos através de estudo histológico e morfométrico. Tatuaram-se duas figuras no dorso de cada animal (n = 35, denominadas controle e experimento. Na figura experimento, aplicaram-se três passadas de laser Er:YAG com parâmetros padronizados. Cinco animais foram eutanasiados nos dias 0, 7, 14, 28, 56, 84 e 112 de pós-operatório. O estudo histológico demonstrou no dia 0 dano de ablação médio de 215,24 micrômetros (mm, sendo homogêneo com vaporização da epiderme e porções superficiais da derme. O dano térmico residual foi pequeno (média de 41,45mm, observando-se áreas onde estava praticamente ausente. A fibroplasia cicatricial já estava presente no dia 7 (média de 55,58mm, aumentando rapidamente até o dia 28 (média de 209,49mm, quando atingiu um platô e se manteve até o dia 112. Não houve sinais de processo inflamatório agudo no dia 7 e a reepitelização já estava completa. Concluiu-se que a aplicação de laser na pele de ratos induz reparação cutânea ao longo de 112 dias nas condições do experimento.This study analyses the skin contracture effect of the erbium:YAG (Er:YAG laser on the skin of rats by light microscopic histological and morphometrical evaluations. Two squares of back skin were precision tattooed on each of the animals (n = 35, that were termed control and experimental skin. The experimental skin was treated with three passes of the Er:YAG laser with standardized parameters. Five animals were killed and skin specimens were analyzed at 00, 07, 14, 28, 56, 84 and 112 days. Histologic examination demonstrated a mean of 215.24 micrometers ablative damage that was homogeneous with complete vaporization of the epidermis and superficial dermis. The residual thermal damage was small (mean of 41.45 micrometers observing areas where it was absent. The cicatritial fibroplasy was present at

  10. Application of relay expansion for large cicatricial alopecia%接力扩张法在大面积瘢痕性秃发修复中的应用

    刘烨; 朱飞; 宁金龙; 汪垠


    To investigate the clinical effect of relay expansion for large cicatricial alopecia. A single surgery was divided into two stages: In stage I , several soft tissue expanders were inserted in subgaleal plane, which would gradually spread the normal scalp organization; In stage II , the expanders were removed, and advanced expansion and rotation flaps were used to repair larger cicatricial alopecia. The surgery was relayed to repair the remaining large cicatricial alopecia with satisfactory result.%探讨应用接力扩张法修复大面积瘢痕性秃发的临床效果.手术分期进行:Ⅰ期按设计在头皮帽状腱膜下埋置扩张器,缓慢扩张正常头皮组织;Ⅱ期取出扩张器,推进或旋转扩张皮瓣修复部分瘢痕性秃发区,同时于头皮瓣下接力置放扩张器,待切口愈合良好后注水扩张;扩张过程完成后再次手术修复残余瘢痕秃发区.以此方法重复进行,直至瘢痕完全去除,效果满意.

  11. 高密度毛发移植术在瘢痕性脱发治疗中的应用%Application of high-density hair transplantation in treatment of cicatricial alopecia

    张菊芳; 沈海燕; 韩蕾; 付丽; 郭晓波; 祝飞; 李金晟


    Objective To investigate the effect of high-density hair transplantation on the treatment of cicatricial alopecia and its features. Methods The units of transplanted hair follicle were counted according to the area of cicatricial alopecia and required hair density. Scalp strip from the occipital region ( donor site) was harvested, and then it was transplanted into the recipient sites ( cicatricial alopecia) after the taken scalp was made into hair follicle units with anatomic structure reservation by microscopic dissection. From January 2005 to December 2010 , totally 76 patients with cicatricial alopecia underwent the operation metioned above. Results After 6 ~ 18 months follow-up in 76 patients , excellent results ( complete cover) were achieved in 36 patients, good ( basic cover) in 24 patients, so-so (lower-density transplanted hair or part uncovered cicatricial alopecia ) in 16 patients. All cases were satisfied with the results except for 2 patients with epidermoid cysts which were healed with local treatments. Conclusion The high-density hair transplantation is a good method for treating cicatricial alopecia with such advantages as slight invasion, quick recovery , high transplanting density , high survival rate and satisfactory postoperative results. It is worth extending in clinic.%目的 探讨高密度毛发移植术在瘢痕性脱发修复中的疗效及其特点.方法 首先根据脱发区的面积及毛发的移植密度计算出移植毛囊的单位数;然后以患者的枕后部为供区,切取优势的头皮条,采用显微分割的方法将其制成保留正常解剖结构的毛囊单位后植入脱发区.自2005年1月至2010年12月,对76例瘢痕性脱发患者行高密度毛发移植术.结果 术后随访76例患者6~18个月,其中脱发区被完全覆盖者36例,为优;基本覆盖者24例,为良;毛发的密度较低,仍有部分脱发区外露者13例,为可;头皮瘢痕明显外露者3例,为差.移植后的

  12. Riesgo de daño renal cicatrizal después de infección del tracto urinario en recién nacidos Risk of cicatricial renal damage after urinary tract infection in newborns

    Manuel Díaz Alvarez


    Full Text Available El presente trabajo se realizó con el objetivo de determinar la prevalencia de daño renal cicatrizal e identificar los factores de riesgo a él contribuyentes en niños recién nacidos con la primera infección del tracto urinario. Se llevó a cabo un estudio analítico de factores de riesgo, caso-control, con regresión logística binominal, en recién nacidos con infección del tracto urinario de localización alta, adquirida en la comunidad, que fueron ingresados consecutivamente en el Hospital Pediátrico Universitario «Juan M. Márquez», entre febrero de 1992 y diciembre de 2004. Se realizó gammagrafía renal con DMSA para identificar cicatrices renales. Las pruebas de chi cuadrado y regresión logística se aplicaron para identificar factores de riesgo independientes. La prevalencia de daño renal cicatrizal fue de 25,4 %. En el modelo de regresión se incluyeron para análisis multivariado los factores de riesgo: ultrasonido prenatal con pielectasia, microorganismo diferente de Escherichia coli, ultrasonido renal posnatal con anomalías, presencia de reflujo vesicoureteral de cualquier grado, reinfección en los primeros 3 meses de vida, sexo masculino, retardo en inicio del tratamiento antibiótico ≥ 4 d, leucocituria ≥ 10 000/mL, respuesta desfavorable al tratamiento inicial y bacteriemia al mismo microorganismo de la infección urinaria. Finalmente solo resultaron significativos (p The present paper was aimed at determining the prevalence of cicatricial renal damage and to identify the risk factors contributing to it in newborns with urinary tract infection for the first time. An analytical case-control study of the risk factors was conducted by binominal logistic regression in newborns with an upper urinary tract infection acquired in the community that were consecutively admitted in “ Juan Manuel Márquez” University Children Hospital from February 1992 to December 2004. A renal scintigraphy with DMSA was performed to

  13. Morfometria de fibroblastos e fibrócitos durante o processo cicatricial na pele de coelhos da raça Nova Zelândia Branco tratados com calêndula Morphometry of fibroblasts and fibrocytes during wound healing in the skin of rabbits of the New Zeland White breed treated with marigold

    Leonardo de Oliveira Pagnano


    Full Text Available O objetivo deste estudo foi avaliar a capacidade cicatrizante da calêndula (Calendula officinalis L. sobre feridas cutâneas experimentais, em 15 coelhos, distribuídos em três grupos denominados: excipiente, calêndula e controle. Cada animal foi submetido à uma incisão cirúrgica de 6cm de comprimento, lateral à coluna vertebral e suturada no padrão U. Os produtos avaliados foram colocados sobre as incisões durante sete dias na quantidade de 0,1ml (loção cremosa não-iônica - grupo excipiente; tintura de calêndula a 5% - grupo calêndula e nos animais do grupo controle não se utilizou nenhum produto. A biópsia de pele foi realizada no 1°, 3°, 5° e 7° dia após a incisão cirúrgica para avaliação morfométrica do processo cicatricial, analisando-se o número de fibroblastos e fibrócitos. A morfometria foi realizada por meio de microscópio óptico adaptado a um sistema computadorizado de análise de imagens. De acordo com os resultados, a calêndula propiciou obtenção dos maiores valores médios das células envolvidas no processo cicatricial, os fibroblastos, deduzindo que a mesma, inferiu uma resposta mais satisfatória na cicatrização em relação aos demais tratamentos.The aim of this study was to evaluate the scarring capability of marigold (Calendula officinalis L. on experimental skin wounds in 15 rabbits, distributed in three groups: excipient, marigold and control. Each animal was subjected to a surgical incision measuring 6cm in length, laterally to the spinal column and sutured in U-shape. Products evaluated were placed on the incisions for 7 days, at a rate of 0.1ml (nonionic creamy lotion - excipient group; 5% marigold extract and no treatment was provided to control animals. Skin biopsy was performed on 1, 3, 5, and 7 days after wounding, for morphometric and cicatricial process evaluations. The morphometry was performed with an optical microscope adapted to a computadorized picture analysis system. The

  14. Avaliação macroscópica, microscópica e histomorfométrica do tecido cicatricial pós-cirúrgico de eqüinos submetidos a duas técnicas de neurectomia digital Macroscopic, microscopic, and histomorphometric evaluation of post-operative scar tissue in horses submitted to two techniques for digital neurectomy

    A. Escobar


    Full Text Available Avaliou-se macro e microscopicamente o tecido cicatricial pós-operatório de eqüinos submetidos a duas técnicas de neurectomia digital: guilhotina (TG e stripping (TS. Decorridos 14 meses das cirurgias, foram colhidas 32 amostras de tecido cicatricial em quatro éguas, que tiveram os membros submetidos a ambas as técnicas. À macroscopia, verificaram-se as dimensões da cicatriz do coto proximal e a distância entre os cotos proximal e distal. À microscopia, foi quantificada a proporção de tecido nervoso regenerado por meio de histomorfometria. Não houve diferença nas dimensões do tecido cicatricial, contudo a distância entre cotos foi 5,6 vezes maior na TS (PThe post-operative healed tissues in horses submitted to two digital neurectomy techniques, the guilhotine (GT and the stripping (ST, were evaluated by macroscopy and microscopy. Fourteen months after surgery, 32 samples of scar tissue were collected from four mares that had the members experimentally submitted to both surgical techniques. By macroscopy, the dimensions of the scar tissue of the proximal stump and the distance between nerve stumps were taken. By microscopy, the proportion of nervous tissues in the scar tissue was quantified by histomorphometry. There were no differences between the scar tissue dimensions, but the distance between stumps was 5.6-fold greater in ST subjects. Histologically, connective tissue, macrophages, and thin nervous fibers were observed in scar tissue present in animals of both groups. Nodular structures composed by nervous fascicules were visualized in 56.2% (9/16 of the samples collected from the ST group. The mean percentage of the nervous tissue in scar tissue was 0.31% in GT samples and 2.6% in ST samples (P<0.001. After ST, a longer time to the return of the sensibility may occur due to the greater distance between stumps. However, greater proportion of nervous tissue in the scar tissue suggests that the use of this technique favors

  15. 颈肩峰区跨区供血轴型扩张皮瓣修复颈部瘢痕挛缩%Expanded cervico-acromial skin flap of crossing supply axial pattern for the repair of cervical cicatricial contracture

    胡春梅; 李养群; 唐勇; 杨喆; 赵穆欣; 陈文; 刘媛媛; 许砾思


    Objective To investigate the blood supply of an expanded skin flap from cervico-acromial region and its application in the repair of cervical cicatricial contracture.Methods The expanded skin flap pedicled was prepared with arteries of lateral neck,blood supply crossing and abundant anastomotic branchea of the neck-should blood vessels,the frontal bound of which is clavicle,the underneath bound of which is spine of scapula,and the lateral bound of which is acromion,to repair the large soft tissue defects on neck.Results All the 19 flaps of 15 patients were survived.The maximum size was 23 cm × 16 cm.Postoperative follow-up showed that the cervico-mandibular function and the external appearance of the flaps were satisfactory.And the scar in donor sites seemed to be neglectable.Conclusions The blood supply of the cervico-acromial flap is abundant,the subcutaneous fat and skin is quite thin after expended.We can harvest a large expanded skin flap.Reparation of cervical cicatricial contracture with expanded cervico-acromial flap pedicled with arteries of lateral neck could be recommended.%目的 探讨颈肩峰区跨区供血轴型扩张皮瓣的解剖学特征及应用于修复颈部瘢痕挛缩的临床效果.方法 应用颈横动脉分支和胸肩峰动脉吻合支跨区供血,形成前界为锁骨、后界为肩胛岗、外界为肩峰的颈肩峰区的扩张皮瓣,以颈侧为蒂转移修复较大面积的颈部软组织缺损.结果 15例患者的19个颈肩峰扩张皮瓣(面积最大为23 cm× 16 cm)全部成活,颈部畸形矫正良好.结论 颈肩峰区跨区供血的吻合支丰富,于深筋膜浅层进行扩张后皮下脂肪层及皮肤较薄,可以形成较大面积的扩张皮瓣,以颈横动脉颈段皮支为轴型血管,转移修复颈部较严重的瘢痕挛缩畸形,是一个良好的手术方法.

  16. Retinoblastoma; Le retinoblastome

    Desjardins, L.; Doz, F.; Schlienger, P.; Validire, P.; Quintana, E.; Zucker, J.M. [Institut Curie, 75 - Paris (France)


    Early symptoms of retinoblastoma (leukocoria, strabismus) and the various steps of the diagnosis and differential diagnosis are reviewed. Retrolental fibroplasia, larva migrans, Coats disease, and above all uveitis are the main differential diagnoses. Pathologic features that allow the diagnosis and have a bearing on the prognosis are described. Genetic factors involved in the genesis of retinoblastoma are reviewed, including recent data provides by molecular biology studies of chromosome 13. Currently available treatments include enucleation, external beam radiation, iodine-125 disks, xenon photo-coagulation, cryo-application, chemotherapy, and carbo-platinum combined with diode laser hyperthermia. The indications of each of these methods in intra- and extra-ocular retinoblastomas are discussed, as well as results and complications. Emphasis is put on the high risk of a second cancer. 47 refs., 4 figs.

  17. Retinoblastoma

    Early symptoms of retinoblastoma (leukocoria, strabismus) and the various steps of the diagnosis and differential diagnosis are reviewed. Retrolental fibroplasia, larva migrans, Coats disease, and above all uveitis are the main differential diagnoses. Pathologic features that allow the diagnosis and have a bearing on the prognosis are described. Genetic factors involved in the genesis of retinoblastoma are reviewed, including recent data provides by molecular biology studies of chromosome 13. Currently available treatments include enucleation, external beam radiation, iodine-125 disks, xenon photo-coagulation, cryo-application, chemotherapy, and carbo-platinum combined with diode laser hyperthermia. The indications of each of these methods in intra- and extra-ocular retinoblastomas are discussed, as well as results and complications. Emphasis is put on the high risk of a second cancer. 47 refs., 4 figs

  18. Cicatrici: El secreto de sus ojos di Sacheri e Campanella

    Sandro Volpe


    Full Text Available In 2005 the Argentinian writer Eduardo Sacheri published The Question in Their Eyes (La pregunta de sus ojos.  The novel narrates on a double level, both fictional and temporal, the decision of a retired prosecutor to come to terms with a case of rape and murder which had taken place years earlier: his life had been shattered by the consequences of that tragedy, and writing about it would be a way to start living again.  The director Juan José Campanella - widely famous for having directed episodes of the television series Law & Order, but especially as the director of successful films such as El hijo de la novia - decided to adapt the novel and involved Sacheri in the project: the close collaboration between writer and director produced a bold screenplay that adds to the noir thriller a more romantic dimension.  Disproving the commonplace of the writer jealous of his work, they wrote together a new version which retains certain elements of the original text, but also introduces a number of relevant changes to the structure, characters and ending of the novel.  The Secret in Their Eyes  (El secreto de sus ojos, shot in 2008, was released the following year and in 2010 won the Academy Award for best foreign language film.

  19. Nonviable Staphylococcus aureus and its peptidoglycan stimulate macrophage recruitment, angiogenesis, fibroplasia, and collagen accumulation in wounded rats.

    Kilcullen, J K; Ly, Q P; Chang, T H; Levenson, S M; Steinberg, J J


    We have previously shown that local application at the time of operation of Staphylococcus aureus, nonviable S. aureus, its cell wall, or S. aureus peptidoglycan accelerates wound healing. We hypothesized that this effect is due to both direct and indirect mechanisms, among which is an increase in the inflammatory response to wounding, resulting in an increase in macrophages, angiogenesis, and fibroblasts. Twenty-seven Sprague-Dawley male rats were anesthetized, and two 7-cm paravertebral skin incisions were made. Four polyvinyl alcohol sponges, two on each side, containing either 100 microliter of isotonic saline or 0.5 mg of nonviable S. aureus or S. aureus peptidoglycan in 100-microliter saline were implanted subcutaneously. Nonviable S. aureus or S. aureus peptidoglycan (860 microgram/cm incision) in 200-microliter saline were inoculated into the incisions at closure. The rats ate a commercial rat chow and drank tap water ad libitum throughout. After days 3 and 7 postwounding, rats were euthanized, and tissues were examined for immunohistochemical features of reparative tissue using ED-1, Factor VIII, and vimentin antibodies, markers for monocyte/macrophages, endothelial cells, and mesenchymal cells (including fibroblasts), respectively. Incisions treated with nonviable S. aureus or S. aureus peptidoglycan showed more macrophages along and deep in the wound tract 7 days postoperatively. Nonviable S. aureus or S. aureus peptidoglycan-treated sponges were surrounded and penetrated by much larger capsules of reparative tissue than saline-treated sponges at both 3 and 7 days. Neutrophil influx was much greater in nonviable S. aureus or S. aureus peptidoglycan-treated sponges, especially in central regions, and there were many more ED-1-stained macrophages in distinct geographic locations, specifically, the more peripheral-cortical areas. Some clustering of macrophages occurred around areas of invasion by reparative tissue into the surrounding subcutaneous fat and within the interstices of the sponges at the interface between reparative tissue and acute inflammatory cells. In contrast, saline-treated sponge reparative tissue had significantly fewer macrophages, much thinner and flimsy reparative tissue, with proportionately fewer macrophages clustering centrally. There were many more mesenchymal cells (notably fibroblasts) and new blood vessels and much more reparative collagen in the nonviable S. aureus or S. aureus peptidoglycan-treated sponges. We conclude that local application of nonviable S. aureus or S. aureus peptidoglycan at wounding induces an increased number and alteration in location of macrophages, increased influx (or proliferation) of mesenchymal cells (notably fibroblasts), and increased angiogenesis and reparative collagen accumulation, as well as increasing the overall acute inflammatory response to wounding. PMID:9776858

  20. Computed tomography of orbital diseases in childhood

    We analyzed computed tomography (CT) of 29 cases of various orbital diseases in pediatric age group. Diagnosis were confirmed by either operation or direct ophthalmoscope. The patients were examined during the period of last 3 years. The results were as follows: 1. Of 29 cases, 21 were male and 8 were female, and the age ranged from 20 days to 15 years. 2. Intraorbital tumors were 13 cases consisting of 7 retinoblastoma, 3 pseudotumor, and 1 each of congenital teratoma, cavernous lymphangioma, and optic glioma. Of remaining 16 cases, 6 had orbital fracture, 5 persistent hyperplastic primary vitreous (PHPV), 3 vitreous opacity, and 2 primary glaucoma. 3. The CT findings of the retinoblastoma were a lobulated or oval soft tissue mass density (40-60 HU) extending into the vitreous without significant enhancement on postcontrast scan. Of 7 cases, 4 had calcifications within the tumors. The extraglobal extension of the tumor was shown in 2 patients demonstrating markedly thickened sclera with enhancement and dilated optic nerve. 4. There were 5 patients with PHPV having a history of white pupil since birth, and all were affected unilateraly. Four patients had vitreous opacity and 3 of those showed either liner or branching tree-like densities within the vitreous on the postcontrast scan. Another one had a lobulated increased density protruding into the vitreous which was difficult to differenciate from the similar finding of retinoblastoma. Remaining one case demonstrated a deformed and decreased density in the lesional lens without change in the density of the vitreous. This was confirmed to be caused by persistence of the embryonic hyaloid artery attached to the posterior capsule of he lens. 5. There were 3 patients with vitreous opacity and 2 of them were due to retinal detachment and 1 was caused by retrolental fibroplasia. The CT findings of retinal detachment were homogeneous or heterogenous opacities in the vitreous. In a case of retrolental fibroplasia

  1. Retinopathy of prematurity: Past, present and future

    Shah, Parag K; Prabhu, Vishma; Karandikar, Smita S; Ranjan, Ratnesh; Narendran, Venkatapathy; Kalpana, Narendran


    Retinopathy of prematurity (ROP) is a vasoproliferative disorder of the retina occurring principally in new born preterm infants. It is an avoidable cause of childhood blindness. With the increase in the survival of preterm babies, ROP has become the leading cause of preventable childhood blindness throughout the world. A simple screening test done within a few weeks after birth by an ophthalmologist can avoid this preventable blindness. Although screening guidelines and protocols are strictly followed in the developed nations, it lacks in developing economies like India and China, which have the highest number of preterm deliveries in the world. The burden of this blindness in these countries is set to increase tremendously in the future, if corrective steps are not taken immediately. ROP first emerged in 1940s and 1950s, when it was called retrolental fibroplasia. Several epidemics of this disease were and are still occurring in different regions of the world and since then a lot of research has been done on this disease. However, till date very few comprehensive review articles covering all the aspects of ROP are published. This review highlights the past, present and future strategies in managing this disease. It would help the pediatricians to update their current knowledge on ROP. PMID:26862500

  2. [Transurethral prostate resection prior to kidney transplantation leading to urethral cicatricial tissue].

    Schou-Jensen, Katrine; Mohammad, Wael


    In Denmark, kidney transplantations in patients above 50 years have increased during the last decade. Consequently, the number of patients with lower urinary tract symptoms due to prostate hypertrophy increases accordingly. We describe two patients, who both had a resection of the prostate while having anuria and waiting for a kidney transplantation from a deceased donor. In both cases it was impossible to place a urethral catheter during the following transplantation due to total urethral occlusion, so a suprapubic catheter was inserted until the scar tissue was dilated or resected by a later transurethral intervention. PMID:25612989

  3. Biliary tuberculosis causing cicatricial stenosis after oral anti-tuberculosis therapy

    Iwai, Tomohisa; Kida, Mitsuhiro; Kida, Yoshiki; Shikama, Nobuaki; Shibuya, Akitaka; Saigenji, Katsunori


    A 36-year-old Philippine woman presented with dark urine and yellow sclera. Endoscopic retrograde cholangiopancreatography (ERCP) confirmed dilatation of the intrahepatic bile ducts and also showed an irregular stricture of the common hepatic duct at the liver hilum. Histological examination of biopsies from the bile duct revealed epithelioid cell granulomas and caseous necrosis. Tubercle bacilli were then detected on polymerase chain reaction (PCR) testing of the bile, giving the diagnosis o...

  4. [Expanded scalp flap in cicatricial areas affected by burns sequelae - an observation].

    El Mazouz, S; Hafidi, J; Fejjal, N; Mejjati, H; Cherkab, L; Gharib, N; Abbassi, A


    Les séquelles esthétiques des alopécies cicatricielles sur séquelles de brûlures sont responsables de préjudices empêchant parfois la réinsertion sociale du patient, surtout chez les sujets de sexe féminin. Le cuir chevelu permet la réalisation de lambeaux permettant de couvrir ces alopécies. Les Auteurs décrivent le cas d'une jeune patiente victime d'une alopécie cicatricielle sur séquelles de brûlures chez qui ils ont réalisé un lambeau expansé du cuir chevelu et mettent le point sur la prise en charge de ces lésions à travers ce cas clinique et une revue de littérature. PMID:21991195

  5. Pharmacological consequences of oxidative stress in ocular tissues

    Ohia, Sunny E. [Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, 141 Science and Research Building 2, University of Houston, Houston, TX 77204 (United States)]. E-mail:; Opere, Catherine A. [Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University Medical Center, Omaha, NE 68178 (United States); LeDay, Angela M. [Professional and Scientific Relations, Procter and Gamble Pharmaceuticals Inc., Mason, OH 45040 (United States)


    The eye is a unique organ because of its constant exposure to radiation, atmospheric oxygen, environmental chemicals and physical abrasion. That oxidative stress mechanisms in ocular tissues have been hypothesized to play a role in diseases such as glaucoma, cataract, uveitis, retrolental fibroplasias, age-related macular degeneration and various forms of retinopathy provides an opportunity for new approaches to their prevention and treatment, In the anterior uvea, both H{sub 2}O{sub 2} and synthetic peroxides exert pharmacological/toxicological actions tissues of the anterior uvea especially on the sympathetic nerves and smooth muscles of the iris-ciliary bodies of several mammalian species. Effects produced by peroxides require the presence of trace amounts of extracellular calcium and the functional integrity of mitochondrial calcium stores. Arachidonic acid metabolites appear to be involved in both the excitatory action of peroxides on sympathetic neurotransmission and their inhibitory effect on contractility of the iris smooth muscle to muscarinic receptor activation. In addition to the peroxides, isoprostanes (products of free radical catalyzed peroxidation of arachidonic acid independent of the cyclo-oxygenase enzyme) can also alter sympathetic neurotransmission in anterior uveal tissues. In the retina, both H{sub 2}O{sub 2} and synthetic peroxides produced an inhibitory action on potassium depolarization induced release of [{sup 3}H] D-aspartate, in vitro and on the endogenous glutamate and glycine concentrations in vivo. Effects caused by peroxides in the retina are mediated, at least in part, by second messengers such as nitric oxide, prostaglandins and isoprostanes. The ability of H{sub 2}O{sub 2} to alter the integrity of neurotransmitter pools from sympathetic nerves in the anterior uvea and glutaminergic nerves in the retina could underlie its role in the etiology of glaucoma.

  6. Pharmacological consequences of oxidative stress in ocular tissues

    The eye is a unique organ because of its constant exposure to radiation, atmospheric oxygen, environmental chemicals and physical abrasion. That oxidative stress mechanisms in ocular tissues have been hypothesized to play a role in diseases such as glaucoma, cataract, uveitis, retrolental fibroplasias, age-related macular degeneration and various forms of retinopathy provides an opportunity for new approaches to their prevention and treatment, In the anterior uvea, both H2O2 and synthetic peroxides exert pharmacological/toxicological actions tissues of the anterior uvea especially on the sympathetic nerves and smooth muscles of the iris-ciliary bodies of several mammalian species. Effects produced by peroxides require the presence of trace amounts of extracellular calcium and the functional integrity of mitochondrial calcium stores. Arachidonic acid metabolites appear to be involved in both the excitatory action of peroxides on sympathetic neurotransmission and their inhibitory effect on contractility of the iris smooth muscle to muscarinic receptor activation. In addition to the peroxides, isoprostanes (products of free radical catalyzed peroxidation of arachidonic acid independent of the cyclo-oxygenase enzyme) can also alter sympathetic neurotransmission in anterior uveal tissues. In the retina, both H2O2 and synthetic peroxides produced an inhibitory action on potassium depolarization induced release of [3H] D-aspartate, in vitro and on the endogenous glutamate and glycine concentrations in vivo. Effects caused by peroxides in the retina are mediated, at least in part, by second messengers such as nitric oxide, prostaglandins and isoprostanes. The ability of H2O2 to alter the integrity of neurotransmitter pools from sympathetic nerves in the anterior uvea and glutaminergic nerves in the retina could underlie its role in the etiology of glaucoma

  7. Percutaneous transhepatic cholangiobiopsy to determine the pathological cause of anastomotic stenosis after cholangiojejunostomy for malignant obstructive jaundice

    Aim: To investigate the feasibility and advantages of cholangiobiopsy during percutaneous transhepatic cholangiography in the histopathological diagnosis of anastomotic stenosis after cholangiojejunostomy for malignant obstructive jaundice. Materials and methods: Using biopsy forceps, specimens were collected from the site of stenosis in patients with recurrent jaundice (n = 24) who had previously undergone cholangiojejunostomy for malignant obstructive jaundice. Results: Stenosis occurred in all patients at the biliary–enteric anastomosis based on percutaneous transhepatic cholangiography, and was the location of the biopsy. Satisfactory specimens were obtained from 22 out of 24 patients. The sensitivity was 91.7% (22/24). Tumour tissue was obtained in 18 cases, confirming disease recurrence. Histopathological changes in four patients were diagnosed as fibroplasia and/or inflammation. These were considered cicatricial stenoses based on histopathological, imaging, and laboratory findings. The remaining two histopathology-negative patients were proven to have recurrent tumour based on imaging, laboratory, and follow-up data. No complications occurred during biopsy, including gastrointestinal haemorrhage or perforation. Either cholangial drainage and/or an inner stent was used following biopsy, which resulted in a noticeable decrease in jaundice postoperatively (p < 0.05). Conclusion: Percutaneous transhepatic cholangiobiopsy using biopsy forceps for the diagnosis of anastomotic stenosis after cholangiojejunostomy for malignant biliary obstructive jaundice is easy to perform and safe, with a high level of sensitivity. Interventional therapies, such as percutaneous transhepatic cholangial drainage and stent placement, can be performed concurrently, markedly improving the symptoms of patients with obstructive jaundice

  8. Radio-induced superficial fibrosis: investigation of the activation mechanisms of the myo-fibroblast and characterization of the cicatricial epidermis

    Whereas radio-induced cutaneous fibrosis is one of the frequent after-effects of accidental and therapeutic irradiations, this research thesis addresses the mechanisms which govern the activation of the myo-fibroblast. After some results obtained on cells from a radio-induced fibrosis on swine cells, the author proposes a signalling alteration as a mechanism. In a model a reconstructed skin, the author shows that myo-fibroblasts are a direct target of Superoxide Dismutase (SOD), and respond to this anti-fibrosis agent by a phenotype reversion. She reports the molecular characterization of epidermis of fibro-necrosis human lesions induced by an accidental or therapeutic irradiation. This leads to a better understanding of the role of the myo-fibroblast during the development and regression of fibrosis. Besides, the author shows that an alteration of the epidermis adjacent to dermis is developing in parallel with the fibrosis process. This suggests an active contribution of keratinocytes during the development of this radio-induced after-effect

  9. Técnicas Utilizadas na Aferição de Feridas e Avaliação do Processo Cicatricial



    Este trabajo tiene como objetivo describir las técnicas de medición de lesiones y crónicas agudas de piel, pre-sentando también sus ventajas y desventajas. Los datos fueron descritos a partir de la experiencia de los mae-stros, enfermeros y alumnos en el Ambulatório de Cirurgia del Hospital Antonio Pedro con los portadores de heridas. Se destacan las facilidades y dificultades encontrados en el desarrollo de cada técnica. La observación sistematiza-da, detallada de las lesiones con la transfe...

  10. Estudio experimental del comportamiento y cicatrización de las anastomosis intestinales (colónicas) efectuadas en condiciones de isquemia con especial atención a la influencia de la pentoxifilina en el proceso cicatricial

    Parra Membrives, Pablo


    El principal problema causante de morbi-mortalidad al que se enfrenta el cirujano digestivo viene dado por las complicaciones relacionadas con las dehiscencias anastomóticas, y de modo particular las que afectan al colon y recto. Existen numerosos factores citados como causantes de la aparición de una fuga anastomótica. La mayoría de las causas señaladas, sin embargo, conducen finalmente a un cierto grado de isquemia o hipoperfusión, y, en el peor de los casos, a un evento de lesión por isque...

  11. Trichoscopy update 2011

    Rudnicka, Lidia; Olszewska, Małgorzata; Rakowska, Adriana; Slowinska, Monika


    Trichoscopy performed with a handheld dermoscope or a videodermoscope became an indispensable tool in differential diagnosis of hair and scalp diseases. Current research is focusing on trichoscopy of: 1) non-cicatricial alopecia, 2) cicatricial alopecia, 3) hair shaft disorders, and 4) inflammatory scalp diseases. This review summarizes current knowledge in these four fields of research. In all non-cicatricial alopecias presence of empty follicular openings is a common trichoscopy finding. In...

  12. Graham Little Piccardi Lasseur Syndrome: A Rare Case Report with Concomitant Hypertrophic Lichen Planus

    Brar, BK; Khanna, Era; Bharat B Mahajan


    Graham Little Piccardi Lasseur Syndrome (GLPLS) is a rare variant of lichen planopilaris comprising of a triad of multifocal and patchy cicatricial alopecia of scalp, non-cicatricial alopecia of axillae and pubic region and keratotic follicular papules over a body. Its exact etiology is not known until date, but primarily involves an immune mediated inflammatory reaction against the bulge region of hair follicles resulting in cicatricial alopecia. We report an extensive and diffuse presentati...

  13. Morfometria de fibroblastos e fibrócitos durante o processo cicatricial na pele de coelhos da raça Nova Zelândia Branco tratados com calêndula Morphometry of fibroblasts and fibrocytes during wound healing in the skin of rabbits of the New Zeland White breed treated with marigold

    Leonardo de Oliveira Pagnano; Silvana Martinez Baraldi-Artoni; Maria Rita Pacheco; Edanir dos Santos; Daniela Oliveira; Jeffrey Frederico Lui


    O objetivo deste estudo foi avaliar a capacidade cicatrizante da calêndula (Calendula officinalis L.) sobre feridas cutâneas experimentais, em 15 coelhos, distribuídos em três grupos denominados: excipiente, calêndula e controle. Cada animal foi submetido à uma incisão cirúrgica de 6cm de comprimento, lateral à coluna vertebral e suturada no padrão U. Os produtos avaliados foram colocados sobre as incisões durante sete dias na quantidade de 0,1ml (loção cremosa não-iônica - grupo excipiente; ...


    宋慧锋; 柴家科; 陈敏亮; 吴焱秋; 董巍; 张建林; 陈宝驹



  15. Retinopathy of prematurity in a controlled trial of prophylactic surfactant treatment.

    Pennefather, P M; Tin, W.; Clarke, M P; Fritz, S.; Strong, N P


    AIMS: To investigate the incidence of acute and cicatricial retinopathy of prematurity (ROP) in a cohort of premature neonates entered into a randomised, multicentre trial of prophylactic exogenous surfactant for respiratory distress syndrome (RDS) compared with controls receiving surfactant only if severe RDS developed. METHODS: The incidence of acute and cicatricial ROP was assessed in 304 neonates born at less than 30 weeks' gestation in a geographically defined population of approximately...


    Maria Bibiana Leroux


    Full Text Available The trichoscopy has been incorporated as a first hand method in patients consulting for scalp problems. Magnifying glass or digital microscope that permit the direct visualization of the hair shaft and the perifolicullar skin are utilized to diagnose cicatricial and non-cicatricial alopecia. A female patient with an alopecia plaque associated with a scalp dysesthesia in which trichoscopy was very useful in its diagnosis is presented.

  17. Keratosis follicularis spinulosa decalvans associated with acne keloidalis nuchae

    Ilteris Oguz Topal


    Full Text Available Keratosis follicularis spinulosa decalvans (KFSD is a keratinization disorder characterized by diffuse follicular hyperkeratosis, progressive cicatricial alopecia, corneal dystrophy, and photophobia. Acne keloidalis nuchae (AKN is a syndrome of chronic folliculitis that manifests as follicular-based pustules and papules on the occipital region of the scalp, which may eventually lead to cicatricial alopecia. Various diseases such as cutis laksa, deafness, aminoaciduria, mental retardation, and atopy have been reported to be associated with KFSD, but AKN is a rare cutaneous manifestation. Herein, we report the case of a patient with KFSD associated with AKN. He was presented to our clinic with follicular-based pustules and papules that had been progressively advancing for five years that were now manifesting as cicatricial alopecia.

  18. Fibromuscular dysplasia

    Jeunemaitre Xavier; Gimenez-Roqueplo Anne-Paule; Boutouyrie Pierre; Perdu Jérôme; La Batide-Alanore Agnès; Plouin Pierre-François


    Abstract Fibromuscular dysplasia (FMD), formerly called fibromuscular fibroplasia, is a group of nonatherosclerotic, noninflammatory arterial diseases that most commonly involve the renal and carotid arteries. The prevalence of symptomatic renal artery FMD is about 4/1000 and the prevalence of cervicocranial FMD is probably half that. Histological classification discriminates three main subtypes, intimal, medial and perimedial, which may be associated in a single patient. Angiographic classif...

  19. Treatment of hypertension in patients with renal artery stenosis due to fibromuscular dysplasia of the renal arteries

    Chrysant, Steven G; Chrysant, George S.


    Renal artery stenosis (RAS) from fibromuscular dysplasia (FMD) is an uncommon cause of hypertension that affects mostly women. FMD is a noninflammatory vascular disease that predominantly affects mainly the renal arteries, but can also affect arteries in other vascular territories. The most common type of FMD is the media fibroplasia with the characteristic “string of beads” appearance (80-90%), whereas the two other types, the “intimal” and “adventitial” FMD are much less common accounting f...

  20. A Novel Technique for Repositioning Lower Eyelid Fat via the Transoral Approach in Association with Midface Lift

    Mofid, M. Mark


    Background Orbital fat repositioning in association with subperiosteal midface elevation has been variably described via both the transconjunctival and skin muscle flap approaches. Poor visualization, middle and posterior lamellar cicatricial fibrosis, technical difficulty, and incomplete release are disadvantages commonly ascribed to the transconjunctival approach. Lower eyelid malposition and retraction also are commonly seen in association with skin muscle flap approaches. A simple techniq...

  1. Systemic sarcoidosis with necrobiosis lipoidica-like scalp lesions

    Andersen, Klaus Ejner


    A 78-year-old woman is described, having systemic sarcoidosis for 15 years, involving the lungs, lymph nodes and the skin. Over the last 6 years she developed a progressive cicatricial alopecia with histologic changes of the granulomatous type of necrobiosis lipoidica surrounded by sarcoid...

  2. [Humoral nonspecific immunity in patients with post-burn cicatrical strictures of the esophagus].

    Mumladze, R B; Babaian, S S; Bobkov, Iu I; Chernyshev, V S; Taratuta, O V


    The authors have studied the significance of factors of the humoral non-specific defense (HND) in 37 patients with cicatricial constrictions of the oesophagus and stomach. The data obtained were used for choosing the optimum terms for gastrostoma and oesophagoplasty. In 14 patients treatment with lysozyme was performed due to decreased indices of HND. PMID:6857955

  3. Sutureless limited vitrectomy for positive vitreous pressure in cataract surgery.

    Chalam, Kakarla V; Gupta, Shailesh K; Agarwal, Swati; Shah, Vinay A


    A sutureless transconjunctival pars plana vitrectomy with the 25-gauge transconjunctival vitrectomy system is used to facilitate phacoemulsification in eyes with positive posterior vitreous pressure and shallow anterior chamber. Peribulbar local anesthesia is administered. In eyes with shallow anterior chamber, if an injection of a viscoelastic substance through anterior chamber paracentesis fails to deepen the anterior chamber, a limited pars plana vitrectomy is performed to remove a small amount of retro-lental vitreous (approximately 0.2 to 0.3 cc) with a 25-gauge high-speed cutter. Phacoemulsification is subsequently performed. The limited pars plana vitrectomy reduces the chances of intraoperative vitreous loss and suprachoroidal hemorrhage. It also increases the anterior chamber depth, facilitates intraoperative steps such as pupil stretching and capsulorhexis, and results in a phacoemulsification procedure that is less complex and safer. PMID:16355960

  4. Alopecia: evaluation and treatment

    Gordon KA


    Full Text Available Katherine A Gordon, Antonella TostiDepartment of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USAAbstract: Hair loss is a very common complaint. Patients may describe increased shedding and diffuse or localized alopecia. The differential diagnosis of hair loss includes a number of disorders causing cicatricial or noncicatricial alopecias. This paper describes the clinical approaches and diagnostic tests that are useful in the evaluation of patients presenting with alopecia. It also reviews treatments for noncicatricial alopecias, including androgenetic alopecia, alopecia areata, and telogen effluvium, as well as cicatricial alopecias, including lichen planopilaris, its clinical variant frontal fibrosing alopecia, and discoid lupus erythematosus.Keywords: alopecia, evaluation, treatment

  5. The use of autogenous fascia lata to correct lid and orbital deformities.

    Flanagan, J C; Campbell, C. B.


    Autogenous and homologous fascia lata have been used to correct many problems in ophthalmic surgery. This paper has described the use of autogenous fascia late to correct lid retraction secondary to thyroid ophthalmopathy, cicatricial entropion and extruding orbital implants. The surgical procedures have been briefly discussed, as well as the presentation of one clinical example of each procedure the pathologic and immunologic aspects of fascia lata grafts will be reported at a later date.

  6. Abbreviations


    ACAME Association des centrales d’achat de médicaments essentiels (Association of central medical stores for essential generic drugs) AMPOT Association malienne pour la promotion des ophtalmologues traditionnels (Malian association for the promotion of traditional ophthalmologists) CT Cicatricial (scar) trachoma DALY Disability Adjusted Life-Years DDT Dichlorodiphenyltrichloroethane DMT Department of traditional medicine DOTS Directly Observed Therapy Short Course FT Follicular trachoma GIS G...

  7. Clinical And Morphological Androgenic Status Characteristics At Children Suffering From Hypospadias And Its Influence On Results Of Surgical Correction

    F.K. Napolnikov; N.Ju. Raigorodskaya; D. A. Zharkov; E.N. Tsmokalyuk


    The goal of this article is to estimate the androgenic status and analyze its influence on the surgical treatment of hypospadias. From 2000 till 2008 there were 209 children under treatment, whose age varied from 8 months till 15 years old (average age — 4,5+ 1,5). 49 patients were subjected to clinical, humoral and morphological study. Preoperational preparation was carried out by testosterone medications. The comparison group consisted of 10 boys with cicatricial phimosis. The patients with...

  8. Alopecia: evaluation and treatment

    Gordon KA; Tosti A


    Katherine A Gordon, Antonella TostiDepartment of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USAAbstract: Hair loss is a very common complaint. Patients may describe increased shedding and diffuse or localized alopecia. The differential diagnosis of hair loss includes a number of disorders causing cicatricial or noncicatricial alopecias. This paper describes the clinical approaches and diagnostic tests that are useful in the evaluation of patie...

  9. Molecular biological aspects of acquired bullous diseases

    Dabelsteen, Erik


    genes have been cloned. The antigens which react with autoantibodies from patients with bullous pemphigoid, cicatricial pemphigoid, acquired epidermolysis bullosa, and linear IgA disease are all proteins of the hemidesmosome basement membrane complex. Interestingly, most of the antigens also appear to...... be the target for mutations seen in patients with the inherited type of epidermolysis bullosa in which bullous lesions are a prominent clinical feature....

  10. Generalised recessive dystrophic epidermolysis bullosa in two sisters

    Grover Sanjiv


    Full Text Available Two female siblings of a family presented with a mechanobullous disorder since birth. Both had retarded physical development, flaccid bullac, extensive cutaneous erosions and scars, mucosal erosions, milia, corneal haziness, deformed and carious teeth, dystrophic nails, cicatricial alopecia of scalp and positive Nikolsky′s sign. Skin biopsy revealed subepidermal bulla. They were diagnosed as generalized recessive dystrophic epidermolysis bbllosa. They were given oral phcnytoin but failed to show significant response.

  11. Epidermolysis bullosa pruriginosa: A case with a novel mutation and co-existent lichen amyloidosus

    Qiping Chen; Joyce Siong-See Lee; Hong Liang Tey


    Epidermolysis bullosa pruriginosa is a rare variant of dystrophic epidermolysis bullosa characterized by severely pruritic and cicatricial lesions localized to the extensor extremities. We report a Singaporean Chinese male with epidermolysis bullosa pruriginosa with an underlying novel mutation in the COL7A1 gene. A heterozygous acceptor splice site mutation IVS67-1G>T probably led to in-frame skipping of exon 68 (36-basepairs), resulting in a loss of 12 amino acids. Among his three children,...

  12. Frontal Fibrosing Alopecia in a Male Presenting with Sideburn Loss

    AlGaadi, Salim; Miteva, Mariya; Tosti, Antonella


    Frontal fibrosing alopecia (FFA) is a cicatricial alopecia that affects mainly postmenopausal women. It is typically characterized by recession of frontotemporal hairline. Hair loss of eyebrows, eyelashes, axillae, limbs, and pubic area may be observed. Here, we describe a case of FFA in a male patient presenting with only localized loss of sideburns. Skin biopsy findings were consistent with lichen planopilaris. To our knowledge, this is the only reported case of FFA patient with only locali...

  13. Beard reconstruction using hair grafts in a free flap

    Levesque, Andre Y; Schalet, Benjamin J; Dolph, James L


    Hair transplantation using micrografts or minigrafts is a standard procedure used for hair restoration in androgenic, burn scar and cicatricial alopecia. These grafts have also been used to reconstruct the eyebrow, eyelash, mustache, beard and pubic escutcheon. A patient who underwent successful micrograft and minigraft hair transplantation into a free osteocutaneous mandibular flap reconstruction is presented. The patient was very satisfied with his reconstruction, and the hair transplants p...

  14. Frontal Fibrosing Alopecia in a Male Presenting with Sideburn Loss.

    AlGaadi, Salim; Miteva, Mariya; Tosti, Antonella


    Frontal fibrosing alopecia (FFA) is a cicatricial alopecia that affects mainly postmenopausal women. It is typically characterized by recession of frontotemporal hairline. Hair loss of eyebrows, eyelashes, axillae, limbs, and pubic area may be observed. Here, we describe a case of FFA in a male patient presenting with only localized loss of sideburns. Skin biopsy findings were consistent with lichen planopilaris. To our knowledge, this is the only reported case of FFA patient with only localized loss of sideburns. PMID:26180452

  15. Epidermolysis Bullosa Acquisita: Autoimmunity to Anchoring Fibril Collagen

    Chen, Mei; Kim, Gene H; Prakash, Lori; Woodley, David T.


    Epidermolysis bullosa acquisita (EBA) is a rare and acquired autoimmune subepidermal bullous disease of the skin and mucosa. EBA includes various distinct clinical manifestations resembling Bullous Pemphigus, Brunsting-Perry pemphigoid, or cicatricial pemphigoid. These patients have autoantibodies against type VII collagen, an integral component of anchoring fibrils, which are responsible for attaching the dermis to the epidermis. Destruction or perturbation of the normally functioning anchor...

  16. Ocular Graft Versus Host Disease Following Allogeneic Stem Cell Transplantation: A Review of Current Knowledge and Recommendations

    Nariman Nassiri; Medi Eslani; Nekoo Panahi; Shiva Mehravaran; Alireza Ziaei; Djalilian, Ali R.


    Graft versus host disease (GVHD) is a common complication of allogeneic stem cell transplantation (allo-SCT). Ocular GVHD develops in approximately 40-60% of patients following allo-SCT and its most common clinical manifestations include keratoconjunctivitis sicca and cicatricial conjunctivitis. Ocular GVHD may lead to severe ocular surface disease, which can significantly diminish quality of life and restrict daily activities. It is thus important to monitor the condition closely since with ...

  17. Метод лікування застарілого вивиху плеча репозиційною системою зовнішньої черезкісткової фіксації

    Shyika, Andrey; Klepach, Mykola; Bobyk, Vasil


    Neglected shoulder dislocation is a difficult orthopaedic pathology and makes 23.7-25.0 % of all dislocations of the shoulder. Closed reduction of shoulder dislocations without complications is actually impossible because of significant cicatricial changes in the shoulder joint capsule and adjacent anatomical formations. Therefore open reduction becomes necessary, but it often results in disruption of the shoulder joint function. The authors developed a method of closed reduction for neglecte...

  18. The ecology of trachoma: an epidemiological study in southern Mexico

    Taylor, Hugh R.; Velasco, Francisco M.; Sommer, Alfred


    A total of 1097 people in two communities in Chiapas, Mexico, were examined for trachoma, and information was obtained about personal and family hygiene. Trachoma was hyperendemic; approximately 25% of those under 10 years old were found to have significant inflammatory trachoma and almost 100% of those aged over 40 years had cicatricial trachoma, although the prevalence of trachoma differed significantly between the two communities. Risk factor analysis was performed by contingency table ana...

  19. Resection And Reconstructive-plastic Surgery In Treatment Of Extended And Multifocal Cicatrical Tracheal Stenosis

    Otabek Eshonkhodjaev; Shukrat Khudaybergenov; Ortikali Irisov; Nasretdin Tursunov


    Background. Main issues of treatment of patients with cicatricial stenosis of trachea (CST) are to increase its efficacy and safety, as well as to determine indications and contraindications for circular resection and reconstructive interventions on the trachea in patients with extended lesions of trachea, with stenoses involving subvocal cords part of larynx and well as multifocal narrowing of the trachea which could improve treatment results and quality of life of patients, to gain recovery...

  20. Thermal Imaging Assessment of Cicatrical Tissue Capabilities in Facioplasty Planning

    N.L. Korotkova; М.G. Volovik


    The aim of the investigation was to study the capabilities of thermal imaging to estimate the functional reserves of blood supply of facial cicatricial tissues used in skin grafting. Materials and Methods. Using an infrared thermal imager Thermo Tracer ТН-9100 we examined 37 patients with post-burn scars on the face and anterior surface of the neck (total: 126 scar areas planned for flap dissection) and 4 healthy volunteers. After recording a native (original) thermal picture, we carried ...

  1. Thermal Imaging Assessment of Cicatrical Tissue Capabilities in Facioplasty Planning

    N.L. Korotkova


    Full Text Available The aim of the investigation was to study the capabilities of thermal imaging to estimate the functional reserves of blood supply of facial cicatricial tissues used in skin grafting. Materials and Methods. Using an infrared thermal imager Thermo Tracer ТН-9100 we examined 37 patients with post-burn scars on the face and anterior surface of the neck (total: 126 scar areas planned for flap dissection and 4 healthy volunteers. After recording a native (original thermal picture, we carried out a standard local alcohol test with further monitoring of space-time temperature dynamics for 7 min. To develop a criterion of tissue suitability for skin grafting, we calculated ∆Т=Тinit–Тfin, where Тinit was the initial temperature before the test, and Тfin — the temperature on the 7th minute of test recovery. Results. The obtained data on the thermal reaction dynamics of cicatricial tissues enable to evaluate the capability to use these tissues as skin grafting material. The criteria are based on thermal imaging findings of the dynamics of initial temperature recovery of healthy and cicatrical tissues. If the temperature difference by the 7th minute of the examined tissues is below 0.9°С by the end of the alcohol test, the tissues can be used for skin grafting. The temperature recovery lag by more than 1.9°С indicates inapplicability of cicatricial tissues for skin grafting. The temperature difference in the range from 0.9 to 1.9°С should be considered risky. Conclusion. The developed thermal image technique to assess the functional state of facial cicatricial tissue blood supply enables to determine the criteria of cicatrical tissue availability for minor facioplasty, and differentiate suitable and unsuitable tissues.

  2. The Rationale for Treating the Nodule in Dupuytren’s Disease

    Lynn D. Ketchum, MD


    Conclusions: The most effective management of Dupuytren’s disease is early recognition and treatment of the nodule, before the development of a joint contracture, particularly of a proximal interphalangeal joint. As there is evidence of a significant inflammatory role in the development of the nodule, the process of fibroplasia can be minimized by altering the macrophage > fibroblast > collagen cascade by the intralesional injection of a potent anti-inflammatory agent such as triamcinolone, which also blocks tissue inhibitors of collagenase, thus enhancing the action of native collagenase, and reduces the size and firmness of nodules and, at least temporarily, arrests their progression.

  3. Efeito da suplementação nutricional com glicina e glutamina, por via oral, na cicatrização colônica em coelhos Effect of the nutritional supplementation with glicine and glutamine, oral feeding, in the colonic cicatrization in rabbits

    Juliano Alves Figueiredo; Andy Petroianu; Maria Isabel Toulson Davisson Correia; Hilário Antônio de Castro Júnior; Wagner Speschit; Raphael de Oliveira Pessoa Silveira; Cristiana Buzelin Nunes; Marcelo Militão Abrantes


    OBJETIVO: Avaliar o efeito de dois aminoácidos, glicina e glutamina, na epitelização da mucosa colônica, tamanho da área de cicatriz, fibroplasia e resistência tênsil da anastomose intestinal, após colectomia parcial e anastomose término-terminal. MÉTODOS: Quarenta e cinco coelhos adultos, com idade entre 24 e 28 semanas, do sexo masculino, com peso inicial médio de 2.362 g, foram distribuídos aleatoriamente, de forma dupla desconhecida, em cinco grupos, de acordo com o tipo de suplemento. Do...

  4. A large unilateral renal artery aneurysm in a young child

    The case of a 13-month-old boy with fibromuscular dysplasia (FMD) presenting with a large saccular aneurysm of the left renal artery and renovascular hypertension is reported. Renal and intrarenal arteries showed numerous small aneurysms alternating with stenoses. All arterial lesions were localized to the left kidney. After left nephrectomy, the patient's blood pressure normalized. Histopathologic examination of the arteries disclosed changes typical of medial fibroplasias, the most frequently described form of FMD in children. This diagnosis is rewarding as it represents a surgically curable cause of severe hypertension. (orig.)

  5. Informative value of MR tomography using gadolinium-DTPA in the diagnosis of the lumbar post-discotomy syndrome

    Beyer, H.K.; Hoetzinger, H.; Toedt, C.; Oppel, U.


    Our results have shown that MR tomography - especially if gadolinium-DTPA is used as a contrast medium - is an excellent method to differentiate between the various possible causes of the post-discotomy syndrome. In particular, it is definitely possible to differentiate between a relapsed hernia of an intervertebral dis and a postoperative development of cicatricial tissue which is practically inevitable. This differentiation is of vital importance to the clinician, because a second operation should be performed only in case of a relapse, whereas cicatricial tissue is best left alone, because no matter how carefully the operation is performed with preparation of the neural structures, even more extensive cicatricial plates are bound to develop. Instability of the vertebral column in the operated disc segment, or other causes of complaints, can be identified via MR equally well if not better than by other means. Hence, the method should be the method of choice in postdiscotomy syndrome, since it facilitates the difficult choice between conservative therapy and reoperation.

  6. The informative value of MR tomography using gadolinium-DTPA in the diagnosis of the lumbar post-discotomy syndrome

    Our results have shown that MR tomography - especially if gadolinium-DTPA is used as a contrast medium - is an excellent method to differentiate between the various possible causes of the post-discotomy syndrome. In particular, it is definitely possible to differentiate between a relapsed hernia of an intervertebral dis and a postoperative development of cicatricial tissue which is practically inevitable. This differentiation is of vital importance to the clinician, because a second operation should be performed only in case of a relapse, whereas cicatricial tissue is best left alone, because no matter how carefully the operation is performed with preparation of the neural structures, even more extensive cicatricial plates are bound to develop. Instability of the vertebral column in the operated disc segment, or other causes of complaints, can be identified via MR equally well if not better than by other means. Hence, the method should be the method of choice in postdiscotomy syndrome, since it facilitates the difficult choice between conservative therapy and reoperation. (orig.)

  7. Stevens-Johnson syndrome: The role of an ophthalmologist.

    Jain, Rajat; Sharma, Namrata; Basu, Sayan; Iyer, Geetha; Ueta, Mayumi; Sotozono, Chie; Kannabiran, Chitra; Rathi, Varsha M; Gupta, Nidhi; Kinoshita, Shigeru; Gomes, José A P; Chodosh, James; Sangwan, Virender S


    Stevens-Johnson syndrome (SJS) is an acute blistering disease of the skin and mucous membranes. Acute SJS leads to the acute inflammation of the ocular surface and chronic conjunctivitis. If not properly treated, it causes chronic cicatricial conjunctivitis and cicatricial lid margin abnormalities. Persistent inflammation and ulceration of the ocular surface with cicatricial complications of the lids leads to chronic ocular sequelae, ocular surface damage, and corneal scarring. The destruction of the glands that secrete the tear film leads to a severe form of dry eye that makes the management of chronic SJS difficult. The option that is routinely used for corneal visual rehabilitation, keratoplasty, is best avoided in such cases. We describe the management strategies that are most effective during the acute and chronic stages of SJS. Although treatments for acute SJS involve immunosuppressive and immunomodulatory therapies, amniotic membrane transplantation is also useful. The options for visual rehabilitation in patients with chronic SJS are undergoing radical change. We describe the existing literature regarding the management of SJS and highlight recent advances in the management of this disorder. PMID:26829569

  8. Manejo del enoftalmos como secuela de fracturas del complejo cigomático-orbitario con apoyo de estereolitografía Management of enophtalmos as sequelae of zygomatic complex orbital fractures with stereolitography model support

    H. Malagón Hidalgo; F. González Magaña; R.T. Rivera Estolano


    El enoftalmos postraumático se define como la discrepancia entre el volumen orbitario y su contenido; este balance puede verse alterado por múltiples factores, como el secuestro tardío del contenido orbitario por atrapamiento en el sitio de la fractura, herniación del contenido orbitario, necrosis del tejido adiposo orbitario, contracción cicatricial del tejido retrobulbar, pérdida del sistema de suspensión ligamentario del globo ocular y aumento de volumen de la cavidad orbitaria. El present...

  9. O autocuidado para o tratamento de úlcera de perna falciforme: orientações de enfermagem

    Amanda Martins; Debora Galvão Moreira; Emilia Matos do Nascimento; Enedina Soares


    O objetivo deste estudo foi verificar a eficácia do Programa do Autocuidado, pela progressão do processo cicatricial de úlcera da perna falciforme. Trata-se de um estudo longitudinal com abordagem quantitativa realizado com 40 clientes no setor de curativos de uma instituição de saúde pública, especializada no tratamento hematológico, situada no Rio de Janeiro. Para a coleta dos dados foi utilizado um instrumento aplicado no momento das consultas de enfermagem. Os dados foram analisados com o...

  10. Fixação esquelética externa tipo Ia (unilateral-uniplanar) para osteossíntese diafisária de úmero em pombos domésticos (Columba livia) Type Ia (unilateral-uniplanar) external Skeletal fixation for treatment of humeral diaphysis fracture in domestic pigeons (Columba livia)

    Alessandro Moraes Leotte; Alceu Gaspar Raiser; Fabricio Arygoni Braga; Luciene Krauspenhar; Sheyla Francheska Camargo; Fabiola Niederauer Flores; Kléber Gomes; Ricardo Simões Scherer


    Das várias patologias que podem acometer as aves, são mais freqüentes as fraturas de ossos longos das asas e dos membros pélvicos. Este experimento teve como objetivo observar, por meio de exames clínicos, histológicos e radiográficos, a resposta cicatricial óssea ao uso do fixador externo tipo Ia (unilateral-uniplanar) na redução aberta de fratura diafisária, de úmero em pombos domésticos (Columba livia). Foram estudados 14 pombos com peso variando entre 290 e 420 gramas. A fratura foi reali...

  11. Bronchial asthma: correlation of high resolution computerized tomography findings with clinical data

    In this work we did a sectional study of 31 asthmatic patients with several levels of disease severity, which were submitted to high resolution computed tomography of the thorax and spirometry, between the months of July, 1995 and August, 1997. The tomographic findings were correlated with the clinical classification of the patients and the most frequent tomographic findings were bronchial wall thickening, bronchial dilatation, air trapping, centrilobular opacities, cicatricial linear shadows, mucoid impaction, emphysema and atelectasis. In asthmatic patients of long duration we observed small airway disease and irreversible lesions as the predominant findings. In smoking patients there was no high frequency of emphysema. (author)

  12. Imposters of androgenetic alopecia: diagnostic pearls for the hair restoration surgeon.

    Rogers, Nicole


    It is crucial that hair restoration surgeons understand the basic clinical diagnosis and pathologic condition of other hair loss conditions that are not always amenable to successful hair transplantation. In this article nonscarring and scarring mimickers of androgenetic alopecia are discussed. Nonscarring conditions include alopecia areata, telogen effluvium, and tinea capitis. Some of the more common scarring alopecias include lichen planopilaris, frontal fibrosing alopecia, and central centrifugal cicatricial alopecia. Less common inflammatory conditions include pseudopelade of Brocq, discoid lupus erythematosus, and folliculitis decalvans. PMID:24017974

  13. Magnetic resobabce tomography in the diagnosis of vertebroneurologic abnormalities

    A total of 453 patients with various diseases of the spine and cord were examined with the TOMIKON BMT 1100 T tomographer with different pulsed sequences SPIN-echo and GRADIENT-echo, that helped differentiate between the lesions with similar clinical symptoms, such as vertebral disk hernias, extravedullary tumors, cicatricial deformations compressing the dural space and the cord, custous changes etc. Traumatic injuries of the spinal cord. MRT advantages are the possibility of imaging in many projections and visualisation of soft-tissue structures without resorting to invase methods

  14. Alopecia frontal fibrosante: relato de seis casos Frontal fibrosing alopecia: report of six cases

    Fabiane Mulinari-Brenner; Fernanda Manfron Rosas; Maurício Shigueru Sato; Betina Werner


    Alopecia frontal fibrosante é forma progressiva de alopecia cicatricial. Os casos iniciais foram relatados a partir 1994, na Austrália, em pacientes do sexo feminino pós-menopausa. Desde então inúmeros casos foram descritos na literatura sugerindo que ela é mais prevalente do que inicialmente se supunha. Seu curso progressivo se assemelha ao da alopecia androgenética; histologicamente, entretanto, o infiltrado liquenóide é evidente. O artigo relata seis casos brasileiros e discute a alopecia ...

  15. Crecimiento maxilar según severidad de hendidura labial, alveolar y palatina unilateral Maxillary growth according to the severity of unilateral cleft lip and palate

    M.C. Navas-Aparicio


    La inhibición del crecimiento y desarrollo resultante del tratamiento quirúrgico en pacientes con labio y paladar hendido es un tema ampliamente discutido en el mundo. De acuerdo a la literatura, la deficiencia de tejidos, probablemente debida al ancho y la posición del segmento alveolar en la hendidura, es una variable a considerar que afecta al crecimiento del maxilar, el cual también puede verse influido por la corrección quirúrgica del labio, la nariz y el paladar, por tejido cicatricial,...

  16. Erosive pustular dermatosis of the scalp successfully treated with oral prednisone and topical tacrolimus*

    Zahdi, Mariana Ribas; Seidel, Gabriela Bestani; Soares, Vanessa Cristina; de Freitas, Camila Fernanda Novak Pinheiro; Mulinari-Brenner, Fabiane Andrade


    Erosive pustular dermatosis of the scalp is a rare inflammatory disorder of the scalp, affecting elderly patients after local trauma and leading to scarring or cicatricial alopecia. Case Report: An elderly female patient complained of painful pustules on the parietal region bilaterally with progressive enlargement and ulceration. A biopsy suggested erosive pustular dermatosis of the scalp and the patient was treated with prednisone 40 mg/day and 0.1% topical tacrolimus. After 10 weeks complete closure of the eroded areas was observed and a stable scarring alopecia developed. PMID:24173187

  17. Bronchial asthma: correlation of high resolution computerized tomography findings with clinical data; Asma bronquica: correlacao de achados em tomografia computadorizada de alta resolucao com dados clinicos

    Mogami, Roberto [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Servico de Radiologia; Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Radiologia; Kirk, Kennedy; Capone, Domenico [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Servico de Pneumologia; Daltro, Pedro [Instituto Fernandes Figueira, Rio de Janeiro, RJ (Brazil). Servico de Radiologia


    In this work we did a sectional study of 31 asthmatic patients with several levels of disease severity, which were submitted to high resolution computed tomography of the thorax and spirometry, between the months of July, 1995 and August, 1997. The tomographic findings were correlated with the clinical classification of the patients and the most frequent tomographic findings were bronchial wall thickening, bronchial dilatation, air trapping, centrilobular opacities, cicatricial linear shadows, mucoid impaction, emphysema and atelectasis. In asthmatic patients of long duration we observed small airway disease and irreversible lesions as the predominant findings. In smoking patients there was no high frequency of emphysema. (author)

  18. Myopia in premature babies with and without retinopathy of prematurity.

    Nissenkorn, I; YASSUR;, Y.; Mashkowski, D; Sherf, I; Ben-Sira, I


    One hundred and fifty-five premature infants weighing 600-2000 g were followed up during 1974-80 for the presence of retinopathy of prematurity (ROP) and for the existence of myopia. 50% of the premature infants who had ROP were myopic, while only 16% myopic premature infants were found among those who did not have ROP. There was a positive correlation between the degree of myopia and the severity of cicatricial ROP. No difference existed in the frequency and degree of myopia between prematur...

  19. Controversies in intraocular lens implantation in pediatric uveitis.

    Phatak, Sumita; Lowder, Careen; Pavesio, Carlos


    Cataract is one of the most common and visually debilitating complications of pediatric uveitis. It develops as a consequence of chronic inflammation and steroid use and is seen most often in juvenile idiopathic arthritis (JIA)-associated uveitis. Cataract extraction with intraocular lens (IOL) insertion has been carried out with a measure of success in non-uveitic pediatric eyes, but in cases of uveitis, multiple factors affect the final outcome. Chronic inflammation and its sequelae such as band keratopathy, posterior synechiae, and cyclitic membranes make surgical intervention more challenging and outcome less certain. Postoperative complications like increased inflammation, glaucoma, posterior capsular opacification, retrolental membranes, and hypotony may compromise the visual outcome. Early refractive correction is imperative in pediatric eyes to prevent amblyopia. The use of contact lenses and intraocular lenses in pediatric uveitic eyes were fraught with complications in the past. Surgical interventions such as vitreo-lensectomy followed by contact lens fitting and small incision cataract surgery followed by different types of intraocular lenses have been utilized, and many reports have been published, albeit in small patient groups. This review analyzes and discusses the existing literature on intraocular lens implantation in cases of pediatric uveitic cataract surgery. PMID:27009616

  20. Ultrasonographic Feature of Persistent Hyperplastic Primary Vitreous

    Andina Hu; Miner Yuan; Fang Liu; Yu Yang; Xiaoyan Ding; Xiaoqing Pei


    Purpose:To observe the ultrasonographic features of patients with persistent hyperplastic primary vitreous (PHPV). Methods: Thirty-two subjects (34 eyes) diagnosed with PH-PV were evaluated by ultrasonography. Results:.The ultrasonography demonstrated a retrolental mass extending from the optic disc to the posterior lens capsule, manifested as band,.regular triangle,.or inverted triangle shapes..The band-shaped echo was characterized as a linear band extended from the optic disc to the posterior lens cap-sule..The regular triangle-shaped echo was manifested as a membranous septum with a wide base extended from the optic disc to the posterior lens capsule, and the anterior part became narrower..The inverted triangle echo was characterized as a membranous septum with a narrow base extended from the optic disc to the posterior lens capsule,.and the anterior part become wider. Conclusion:.Ultrasonography is noninvasive and safe,.and can offer real-time display of intraocular structures. This is es-pecially important in individuals who are uncooperative or un-suitable for fundus examination due to media opacity. Com-bined with clinical feature, ultrasonography provides vital evi-dence for the diagnosis of PHPV..Thought observing ultra-sonographic feature, clinicians could evaluate the size, posi-tion and severity of lesions in PHPV patients,.and which would be helpful to determine the surgical approach and clini-cal prognosis.

  1. Advances in diagnosis, clinical care, research, and treatment in retinopathy of prematurity

    Hartnett ME


    Full Text Available M Elizabeth Hartnett,1–3 Antonio Capone Jr4,5 1Vitreoretinal Surgery, John A. Moran Eye Center, 2Department of Ophthalmology, 3Department of Neurobiology and Anatomy, and Pediatrics, University of Utah, Salt Lake City, UT, 4Associated Retina Consultants, Royal Oak, 5Department of Ophthalmology, Oakland University William Beaumont Hospital School of Medicine, Auburn Hills, MI, USAThe appearance of retinopathy of prematurity (ROP has changed throughout the world and since the first description of “retrolental fibroplasia” in 1942. However, despite advances in neonatal care and the abilities to improve the survival of ever younger and smaller premature infants, ROP remains a leading cause of childhood blindness worldwide. We know that ROP is complex in that it is influenced by genetic predisposition, epigenetic regulation, and environmental risks. It is strongly associated with extreme degrees of prematurity, and the “phenotype” of ROP depends on resources available to support premature infants with adequate nutrition and regulation of oxygen, as examples. New studies also suggest that what is seen in the preterm infant retina may portend later neurodevelopmental outcomes. Therefore, we believe this is a needed time to revisit ROP and provide a thematic issue focused on ROP from several perspectives.



    930437 A study on the pathologic changes andmechanism of radiation interstitial pneumonitis.BAI Yunhong(白蕴红),et al.Instit RadiationMed,Milit Acad Med Sci,Beijing,100000.Med JChin PLA 1993;18(1):14—18.The right or whole thorax of Wistar rat wasirradiated with Co γ—ray including single expo-sure(10,30, 50,70Gy)and fractional exposures(30,60Gy).Animals from each group were sac-rificed at 0.5,1,2,3,6,9,12 months after irradia-tion.Sections of lung were examined with lightmicroscopy(LM),electron microscopy(EM),histochemistry and morphometry.The pul-monary changes could be divided into four phas-es;exudation,granulation,fibroplasia and colla-genation.The four phases can exactly show.the

  3. 7, 8-dihydroxycoumarin improves neurological function in a mouse model of sciatic nerve injury

    Jianshi Du; Qing Zhao; Yingli Zhang; Yu Wang; Ming Ma


    In the present study, a mouse model of sciatic nerve injury was treated with intraperitoneal injection of 7, 8-dihydroxycoumarin (10, 5, or 2.5 mg/kg per day). Western blot and real-time PCR results showed that growth associated protein 43 expression was significantly increased in the L4-6 segments of the spinal cord. The amplitude and velocity of motor nerve conduction in the sciatic nerve were significantly increased in model mice. In addition, the appearance of the myelin sheath in the injured sciatic nerve was regular, with an even thickness and clear outline, and the surrounding fibroplasia was not obvious. Our results indicate that 7, 8-dihydroxycoumarin can promote the repair of injured nerve by upregulating growth associated protein 43 expression in the corresponding spinal cord segments of mice with sciatic nerve injury.

  4. Skin barrier in rosacea.

    Addor, Flavia Alvim Sant'Anna


    Recent studies about the cutaneous barrier demonstrated consistent evidence that the stratum corneum is a metabolically active structure and also has adaptive functions, may play a regulatory role in the inflammatory response with activation of keratinocytes, angiogenesis and fibroplasia, whose intensity depends primarily on the intensity the stimulus. There are few studies investigating the abnormalities of the skin barrier in rosacea, but the existing data already show that there are changes resulting from inflammation, which can generate a vicious circle caused a prolongation of flare-ups and worsening of symptoms. This article aims to gather the most relevant literature data about the characteristics and effects of the state of the skin barrier in rosacea. PMID:26982780

  5. Splendore-Hoeppli phenomenon in a cat with osteomyelitis caused by Streptococcus species.

    França, Silvia de Araujo; Braga, Juliana Fortes Vilarinho; Moreira, Matheus Vilardo Loes; Silva, Vitor Cesar Martins; Souza, Erick Ferry; Pereira, Luiz Carlos; Rezende, Cleuza Maria de Faria; Ecco, Roselene


    A 9-month-old male neutered mixed-breed cat had a history of chronic lameness of the right hind limb, which was non-responsive to antibiotic treatment. Hematologic analysis revealed marked neutrophilia and mild monocytosis. Radiography revealed extensive loss of cortical bone, and replacement with irregular and disorganized bone. There was loss of the normal cortico-medullary distinction, and the medullary cavity had an irregular radiodensity suggestive of osteomyelitis. Surgical curettage and antibiotics did not improve the clinical condition, and amputation was performed. Grossly, the skin over the right tibia was ulcerated with a viscous and granular exudate. At histopathology, there was marked diffuse pyogranulomatous dermatitis, myositis, periostitis and osteomyelitis associated with Splendore-Hoeppli phenomenon. In addition, there was marrow osteoproliferation and multifocal cortical loss, reabsorption, fibroplasia and endosteal bone formation. Gram staining revealed myriad slightly elongated Gram-positive bacteria, arranged in pairs or single chains, confirmed by polymerase chain reaction as Streptococcus species. PMID:23906707

  6. Hypereosinophilic paraneoplastic syndrome in a cat with intestinal T cell lymphosarcoma.

    Barrs, V R; Beatty, J A; McCandlish, I A; Kipar, A


    A 10-year-old, neutered female, domestic shorthair cat was presented with a recent history of weight loss, polydipsia, diarrhoea and vomiting. On physical examination, intestinal thickening and mesenteric lymph node enlargement were apparent. Clinical investigations revealed peripheral blood eosinophilia, eosinophilic abdominal effusion and eosinophilic mesenteric lymphadenitis. There was a temporary response to treatment with glucocorticoids but signs progressed and the cat was euthanased. On histology, there was eosinophilic infiltration and fibroplasia of intestine and mesenteric lymph nodes. Large aggregates of neoplastic round cells in the intestine and lymph nodes were identified as T lymphocytes using immunohistochemistry. A diagnosis of intestinal T cell lymphosarcoma was made. This case demonstrates that hypereosinophilic paraneoplastic syndrome may occur in cats with lymphosarcoma. Eosinophil chemotaxis may be a response to the production of interleukin-5 by neoplastic lymphocytes. PMID:12238505

  7. Renal Angioplasty in Non-atheromatous Renal Artery Stenosis: Technical Results and Clinical Outcome in 43 Patients

    This study retrospectively reviewed the technical and clinical results of percutaneous transluminal renal artery angioplasty (PTRA) for non-atheromatous renal artery stenosis (RAS) in a Tertiary Renal Referral Centre. Forty-three patients (including 9 children) underwent 49 PTRA procedures for stenoses of 63 arteries over the period 1984-2001 (14 patients had bilateral stenosis treated during one procedure. There were 29 females and 14 males (age range 1-72 years, median 37 years). The etiology of the RAS was classical beaded FMD (medial fibroplasia) in 24, atypical or 'variant FMD' with a more focal stenosis (intimal fibroplasia) in 11, neurofibromatosis type 1 (NF) in 7 and Takayasu's Arteritis in 1. Five of the NF patients had angioplasty for stenoses following vascular repair procedures. A technically good result was obtained in 34/34 arteries with 'classical' RAS, 9/13 atypical arteries, 11/15 arteries of NF patients and in the one Takayasu's case. Clinical follow-up for a mean of 16 months revealed a cure rate of hypertension in classical FMD of 35% with improvement in a further 55%. In the atypical FMD cases, follow-up was obtained on 6 patients with 2 cures and the other 4 demonstrating benefit. There was a better chance of cure in younger patients. In native artery PTRA in children with NF, only 1 out of 3 patients was 'cured' post-PTRA, and 2 out of 3 failed. However, in postsurgical stenoses in NF patients 1 out of 4 patients was 'cured' and 3 out of 4 improved. In conclusion, classical FMD responds well to PTRA with better results in younger patients. Atypical FMD, especially in children and when associated with NF, is less predictable. Stenoses consequent to revascularization surgery respond well to PTRA

  8. Preparation of a small intestinal submucosa modified polypropylene hybrid mesh via a mussel-inspired polydopamine coating for pelvic reconstruction.

    Ge, Liangpeng; Liu, Lubin; Wei, Haoche; Du, Lei; Chen, Shixuan; Huang, Yong; Huang, Renshu


    Pelvic organ prolapse (POP) is a serious health issue that affects many adult women. Surgical treatments for POP patients comprise a common strategy in which scaffold materials are used to reconstruct the prolapsed pelvic. However, the existing materials for pelvic reconstruction cannot meet clinical requirements in terms of biocompatibility, mechanics and immunological rejection. To address these concerns, polypropylene (PP) mesh was selected because of its strong mechanical properties. Small intestinal submucosa (SIS) was used to modify the PP mesh via a mussel-inspired polydopamine coating to enhance its biocompatibility. The scanning electron microscopy (SEM) and atomic force microscopy (AFM) results demonstrated that SIS was successfully conjugated on the surface of the PP mesh. Moreover, the cytotoxicity results indicated that the PP mesh and SIS-modified PP mesh were safe to use. Furthermore, in vivo tests demonstrated that the fibroplasia around the implanted site in the SIS-modified PP mesh group was significantly less than the fibroplasia around the PP mesh group. In addition, the immunohistochemistry staining results indicated that the expression of pro-inflammatory macrophages (M1) was substantially lower and that the expression of pro-healing macrophages (M2) was higher in the SIS-modified PP mesh group. Furthermore, ELISA detection indicated that the expression of IL-1β and IL-6 in the SIS-modified PP mesh group was reduced compared with the PP mesh group. These findings suggest that a SIS-modified polypropylene hybrid mesh via a mussel-inspired polydopamine coating is a promising approach in pelvic reconstruction. PMID:26801474

  9. Preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesion of different types

    Xie-Lai Zhou; Shan-Wen Chen; Guo-Dong Liao; Zhou-Jun Shen; Zhi-Liang Zhang; Li Sun; Yi-Jun Yu; Qiao-Ling Hu; Xiao-Dong Jin


    .378 ±0.043 μg/mg pr, P = 0.387; 4 wk: 0.370 ± 0.032 vs 0.367± 0.041 μg/mg pr, P = 0.853); Pathological changes:In group B, the main pathological changes were fibroplasias in the treated serous membrane surface and in group D, the fibroplasia was shown in the whole layer of the vermiform processes. In group E, the main pathological changes were acute and chronic suppurative inflammatory reactions. These changes were lighter in the experimental subgroups than those in the control subgroups in the three groups. In group C, the main changes were foreign body giant cell and granuloma reactions and fibroplasias in different degrees, with no apparent differences between the experimental and control subgroups.CONCLUSION: The gelatinizedly-modified chitosan film is effective on preventing peritoneal adhesions induced by wound, ischemia and infection, but the effect is not apparent in foreign body-induced adhesion.

  10. The enigma of Serre's "Z-plasty" technique.

    Borges, A F


    In 1835, Serre performed an operation to correct a cicatricial displacement of the right mouth commissure, which he described in his 1842 book. The drawings shown in a plate in the book (Fig. 2) and the description of the technique in the legend depicted the technique he had planned to perform (excision of tissue within lines ABCDE) but was not the technique he actually did. The description of the technique in the text of the book (Fig. 4) is very poor and incomplete. Apparently, he raised a triangular flap, which contained the right commissure, and sutured it into the skin defect on the check that he had created. The Z-plasty technique attributed to him (Fig. 1) in many textbooks is not the technique he had planned nor the technique he performed. He did not describe a Z-plasty but only the transposition of a single flap. PMID:3898170

  11. Squamous cell carcinoma complicating an hereditary epidermo-lysis bullosa

    The dystrophic form of hereditary epidermo-lysis bullosa is associated with an increased frequency of squamous cell carcinoma. We report a new case. An 18-year-old patient, carrying a Hallopeau Siemens hereditary epidermo-lysis bullosa, presented a subcutaneous nodular lesion, for 1 year that ulcerated and budded with inguinal lymphadenopathy. The histological study ted to the conclusion of a well differentiated squamous cell carcinoma. The patient was treated surgically. Tumor and metastatic lymph nodes were excised. A radiotherapy was decided but the postoperative course was fatal due to an infection and to a deterioration of her general condition. Squamous cell carcinoma frequently occurs on the cicatricial lesion of hereditary epidermo-lysis bullosa and usually affects males with recessive hereditary epidermo-lysis bullosa. Metastases are frequent, precocious and multiple. The treatment may be surgical. The particularities of our observation are the young age of patient and the localization. (author)

  12. The Clinical Diagnosis of Autoimmune Bullous Diseases Sürekli Eğitim

    Rıfkiye Küçükoğlu


    Full Text Available The autoimmune bullous diseases were diagnosed on the clinical and histopathological basis, before the introduction of the immunological assays. However, not the clinical features, but the classification of the diseases has recently changed during the immunological development. According to this new classification, pemphigus group diseases include, pemphigus vulgaris, pemphigus vegetans, pemphigus foliaceus, pemphigus erythematosus, endemic pemphigus, IgA pemphigus, pemphigus herpetiformis, paraneoplastic pemphigus, and drug-induced pemphigus. The subepidermal bullous diseases are classified as pemphigoid group diseases, epidermolysis bullosa acquisita, dermatitis herpetiformis, and linear IgA bullous dermatosis. The pemphigoid-group diseases consist of bullous pemphigoid, childhood bullous pemphigoid, localised bullous pemphigoid, drug-induced pemphigoid, anti p200 pemphigoid, pemphigoid gestationes, pemphigoid nodularis, and cicatricial pemphigoid. In this review, the clinical features of the autoimmune bullous diseases are discussed according to the above mentioned classification. (Turkderm 2010; 45 Suppl 1: 16-25

  13. Clinical and morphological evaluation of snake venom derived fibrin glue on the tendon healing in dogs

    G. C. Ferraro


    Full Text Available The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the healing of the deep digital flexor tendon, during three periods. The tendon of the 2nd digit of 30 thoracic limbs of dogs was partially sectioned for glue application. Biopsies were performed 7, 15, and 30 days post surgery for the clinical and morphological study of tendons. Analysis of the results showed that 73.3% of the tendons showed stump retraction and 16.6% moderate to excessive adherence, which affected sliding. There was a significant difference in the number of inflammatory cells among the three studied periods, being the highest on day 15. The morphological analysis revealed a typical tendon healing process with a lower level of inflammation in the acute phase, facilitating the cicatricial maturation phase. Snake venom derived fibrin glue promotes the healing in dog flexor tendon.

  14. History of lumbar disc herniation with Gd-DPTA enhanced MRI

    Iwahara, Toshihito; Satou, Toshikazu [Wakkanai Municipal Hospital, Hokkaido (Japan)


    The pathological changes of primary lumbar disc herniation was monitored on T1-weighted and Gd-weighted MR imagings. The subjects were 11 patients whose symptoms improved solely by conservative therapy. Herniated area on T1-weighted images (whole herniated area), enhanced herniated area on Gd-MRI (enhanced area), and non-enhanced herniated area on Gd-MRI (non-enhanced area) were calculated, and each proportion to the vertebral canal was assessed. Changes in herniated area was correlated with changes of enhanced area. Gd-MRI was found to be useful in predicting the prognosis of primary herniation, as well as in differentiating recurrent herniation and cicatricial tissues. (S.Y.).

  15. Líquido amniótico tópico: uma potencial nova alternativa para doenças da superfície ocular Topical amniotic fluid: a potential new alternative for ocular surface diseases

    Guilherme Goulart Quinto; Suy Anne Rebouças Martins; Renata Tiemi; Mauro Campos; Ashley Behrens


    O líquido amniótico banha o feto durante a vida intra-uterina e está em contato permanente com a superfície ocular durante este importante período do desenvolvimento. Ele contém uma série de fatores de crescimento que podem ter diversos efeitos sobre o processo cicatricial. Estes fatores aceleram a recuperação da sensibilidade corneana e regeneração nervosa após procedimentos cerato-refrativos, além de controlar a formação de cicatriz e o equilíbrio da superfície ocular após sua aplicação tóp...

  16. Perda pilosa por líquen plano pilar após transplante capilar: relato de dois casos e revisão da literatura Hair loss due to lichen planopilaris after hair transplantation: a report of two cases and a literature review

    Márcio Rocha Crisóstomo; Manoela Campos Cavalcante Crisóstomo; Marília Gabriela Rocha Crisóstomo; Victor José Timbó Gondim; Mara Rocha Crisóstomo; André Nunes Benevides


    Alopecia androgenética é tratada com frequência por meio de microtransplante capilar, téc nica em que os fios transplantados geralmente não caem, pois mantêm características da área doadora, mais resistente. O líquen plano pilar é uma alopecia cicatricial com permanente destruição pilosa. Este artigo relata dois casos de lesões compatíveis com líquen plano pilar em áreas receptora e doadora póstransplante. A dominância da área doadora foi aparentemente sobrepujada pelo líquen plano pilar, que...

  17. A case of radiation hemorrhagic gastritis successfully treated by endoscopic argon plasma coagulation

    A 72-year-old woman underwent irradiation of 46 Grey in total dose, for lumbago of the advanced pancreatic cancer in August 2000. She was admitted to our hospital due to severe anemia in February 2001 with occasionally positive fecal occult blood. Endoscopy revealed erosive gastric mucosa diffuse in the lower body of the stomach, which resulted from radiation. We applied argon plasma coagulation (APC) in March 2001 and succeeded in hemostasis of the widely spreading radiation hemorrhagic gastritis. The progress of the severe anemia improved without cicatricial stenosis. As the coagulation of the APC is limited in the surface mucosa, APC is an easy and effective treatment for radiation hemorrhagic gastritis. (author)

  18. Expander Dermatension is a New Method of Surgical Replacement of Skin Defects

    Bogos’yan R.A.


    Full Text Available There have been shown the treatment possibilities of patients with post-burn and post-traumatic cicatricial deformity and defects of various localizations using the method of expander dermatension. The effectiveness of a new way of a dosage dermatension management in a constant mode by liquid pumping into a ballon-expander through infusomat (Patent No.2271157 has been studied. An optimal solution administration rate for different expanders has been selected. There has been described the way of objective control of soft tissue condition during distraction using thermovision studies (Patent No.2306847. The use of the techniques suggested enables to reduce the number of complication on dermatension stage and reduce the period of in-patient treatment by 1.5—2 times.

  19. [The use of a laser in the surgical treatment of an epithelial coccygeal cyst].

    Grubnik, V V; Bakhar, G A


    In acute inflammation of pilonidal sinus (PS), use of a laser scalpel permitted to reduce considerably the incidence of postoperative suppuration. Pain syndrome became less pronounced, or was absent, wound swelling disappeared at the early period. No differences in results of surgical treatment of uncomplicated PS at the stage of chronic inflammation in use of CO2-laser and conventional methods of treatment were noted. An original technique of operation for PS complicated by multiple fistulas, purulent leaks, pyodermia has been suggested. For plastic closure of a wound, the effect of laser photohydraulic preparation was used. Operated on was 31 patient, no disease recurrence was noted. Rough cicatricial deformity of the sacro-coccygeal region was absent. PMID:10912022

  20. Recent advancement on autoantigens, autoantibodies and inflammatory cells in subepidermal autoimmune bullous diseases

    Jianguo Huang; Qiying Gong; Guiming Li


    Subepidermal autoimmune bullous diseases (SABD) are some autoimmune skin diseases that can present in a variety of forms and can be a challenging disease to treat. An overview of the different forms of SABD are discussed including bullous pemphigoid (BP), epidermolysis bullosa acquisita (EBA), cicatricial pemphigoid (CP), bullous systemic lupus erythematosus (BSLE), and Anti-p200 pemphigoid. Emphasis on recent advancement is presented. In recent years, improved Knowledge of the mechanisms of intercellular and cell-matrix adhesion has led to better understanding of the blistering process in some SABD. Defects of such structures cause the subepidermal bullous diseases and have also led to the discovery of new diseases (e.g. anti-p200-pemphigoid). Recent studies have outlined the important role of autoantibodies, mast cell lymphocytes and their cytokines in pathogenesis of SABD.

  1. Perda pilosa por líquen plano pilar após transplante capilar: relato de dois casos e revisão da literatura Hair loss due to lichen planopilaris after hair transplantation: a report of two cases and a literature review

    Márcio Rocha Crisóstomo


    Full Text Available Alopecia androgenética é tratada com frequência por meio de microtransplante capilar, téc nica em que os fios transplantados geralmente não caem, pois mantêm características da área doadora, mais resistente. O líquen plano pilar é uma alopecia cicatricial com permanente destruição pilosa. Este artigo relata dois casos de lesões compatíveis com líquen plano pilar em áreas receptora e doadora póstransplante. A dominância da área doadora foi aparentemente sobrepujada pelo líquen plano pilar, que deve ter gerado a queda dos fios. Relatos semelhantes são raros. À suspeita de líquen plano pilar, devese biopsiar o couro cabeludo e evitar o transplante durante a atividade da doença.Androgenetic alopecia is often treated by follicular unit transplantation, a technique that involves minimal risk of hair loss because of the more resistant nature of the donor area. Lichen planopilaris is a cicatricial alopecia that causes permanent destruction of hair follicles. We report two cases of post-transplantation lesions compatible with lichen planopilaris in both recipient and donor areas. The quality of the hair follicles in the donor area was apparently compromised by lichen planopilaris, the probable cause of hair loss. Similar reports are rare. When lichen planopilaris is suspected, a biopsy of the scalp must be performed to avoid transplantation during disease activity.

  2. Efeitos da luz emitida por diodos (LED e dos compostos de quitosana na cicatrização de feridas - Revisão Sistemática

    Renata Oliveira Moura


    Full Text Available A reparação de feridas constitui um processo complexo, que envolve fatores externos e internos do tecido local, seguindo fases que vão desde a proliferação celular, passando pela angiogênese até o fechamento total da ferida com a reepitelização. A quitosana e a fototerapia por luz emitida por diodo (LED– Light Emiting Diode são duas terapias que estimulam o reparo tecidual. O presente estudo teve como objetivo realizar revisão bibliográfica acerca dos efeitos cicatricial do LED e da Quitosana de forma isolada e associada. Uma busca na literatura foi realizada utilizando uma variedade de fontes, incluindo base de dados on line. A busca em bancos de dados possibilitou a seleção de 15 artigos sobre o efeito do LED na cicatrização e 17 artigos sobre os efeitos cicatrizantes da quitosana. Não foram encontrados nesta revisão artigos que relatassem a associação dos dois procedimentos na cicatrização. Os artigos analisados mostram os efeitos positivos e isolados do LED e de compostos à base de quitosana na aceleração do processo cicatricial. Observou-se falta de padronização nos estudos quanto as variáveis analisadas. Sugerem-se novos estudos que avaliem a eficácia da associação dos dois procedimentos: LED e Quitosana.

  3. Hair follicle stem cells in disorders characterized by alopecia%毛囊干细胞在脱发性疾病中的研究进展

    管海宏; 李泓馨; 林麟


    Alopecia,as a common disease,is generally classified into two groups,cicatricial and noncicatricial alopecia,according to clinical presentations and regrowth capability of hairs,and each group includes various types of alopecia.The pathogenesis of alopecia remains poorly understood.Hair follicle stem cells are located in the bulge area,whose periodic proliferation and differentiation sustain the growth,shedding and regrowth of hairs.Recent research has indicated that the damage or deficiency of hair follicle stem cells may contribute to the pathogenesis of some types of alopecia,especially cicatricial alopecia.%脱发性疾病在临床比较常见,一般按临床表现和毛发是否可再生而将脱发分为瘢痕性脱发和非瘢痕性脱发,每种均包含了多种不同的脱发性疾病.目前脱发性疾病的发病机制仍不甚清楚.毛囊干细胞位于毛囊隆突区,其周期性地增殖和分化维持了毛发的正常生长、脱落与更替.研究表明,毛囊干细胞的损伤或缺失很可能参与了某些脱发性疾病尤其是瘢痕性脱发的发病过程.

  4. Graham-Little Piccardi Lassueur Syndrome: case report Síndrome de Graham-Little Piccardi Lassueur: relato de caso

    Raquel Bissacotti Steglich


    Full Text Available A 33-year-old woman presented with a 3-year history of progressive alopecia of the scalp. Past treatment with hydroxicloroquine did not show improvement. Physical examination revealed multiple areas of alopecia with atrophic aspect of the scalp, and axillary and pubic hypotrichosis. Dermoscopy showed hyperkeratosis and accentuation of follicular ostia. Anatomopathological examination revealed decrease in the number of hair follicles, upper perifollicular infiltrate and areas with fibrosis. The Piccardi-Lassueur-Graham-Little syndrome is a rare disorder, characterized by the triad of multifocal scarring alopecia of the scalp, keratotic follicular eruption and hypotrichosis of axillary and pubic regions. Management is a challenge and many medications tried have controversial results. We report a case of this rare syndrome which improved with corticoids.Mulher, 33 anos, apresenta quadro de alopecia progressiva do couro cabeludo há 3 anos. Tratamento com hidroxicloroquina há 12 meses, sem apresentar melhora. Ao exame físico exibe múltiplas áreas de alopecia cicatricial no couro cabeludo, além de hipotricose axilar e pubiana. A dermatoscopia evidencia hipercetose folicular e acentuação dos óstios foliculares. O exame anatomopatológico revela diminuição do número de folículos pilosos, infiltrado perifolicular e fibrose. A síndrome de Graham-Little Piccardi Lassueur é uma dermatose rara, caracterizada pela tríade de alopecia cicatricial multifocal do couro cabeludo, ceratose folicular disseminada e hipotricose das regiões axilares e pubianas. A terapêutica desta dermatose é um desafio, muitas medicações relatadas tem resultados controversos. Relatamos o caso desta síndrome rara que apresentou melhora com corticoterapia.

  5. Relationship of creatine kinase, aspartate aminotransferase, lactate dehydrogenase, and proteinuria to cardiomyopathy in the owl monkey (Aotus vociferans)

    Gozalo, Alfonso S.; Chavera, Alfonso; Montoya, Enrique J.; Takano, Juan; Weller, Richard E.


    The purpose of this study was to determine serum reference values for crea- tine kinase (CK), aspartate aminotransferase (AST), and lactate dehydroge- nase (LDH) in captive-born and wild-caught owl monkeys to assess their usefulness for diagnosing myocardial disease. Urine samples were also collected and semi-quantitative tests performed. There was no statistically significant difference between CK, AST, and LDH when comparing both groups. However, when comparing monkeys with proteinuria to those without proteinuria, a statistically significant difference in CK value was observed (P = 0.021). In addition, the CK/AST ratio revealed that 29% of the animals included in this study had values suggesting cardiac infarction. Grossly, cardiac concentric hypertrophy of the left ventricle and small, pitted kidneys were the most common findings. Microscopically, myocardial fibrosis, contraction band necrosis, hypertrophy and hyperplasia of coronary arteries, medium-sized renal arteries, and afferent glomerular arteriolae were the most significant lesions, along with increased mesangial matrix and hypercellularity of glomeruli, Bowman’s capsule, and peritubular space fibroplasia. These findings suggest that CK, AST, and LDH along with urinalysis provide a reliable method for diagnosing cardiomyopathies in the owl monkey. In addition, CK/AST ratio, proteinuria, and the observed histological and ultrastructural changes suggest that Aotus vociferans suffer from arterial hypertension and chronic myocardial infarction.

  6. Human umbilical mesenchymal stem cells conditioned medium promote primary wound healing regeneration

    Dwi Liliek Kusindarta


    Full Text Available Aim: This research was conducted to clarify the capability of human umbilical mesenchymal stem cells conditioned medium (HU-MSCM to promote regenerations of primary wound healing on the incision skin injury. Materials and Methods: In this study, two approaches in vitro and in vivo already done. On in vitro analysis, tube formation was performed using HU vein endothelial cells in the presence of HU-MSCM, in some experiments cells line was incubated prior the presence of lipopolysaccharide and HU-MSCM then apoptosis assay was performed. Furthermore, in vivo experiments 12 female rats (Rattus norvegicus were used after rats anesthetized, 7 mm wound was made by incision on the left side of the body. The wound was treated with HU-MSCM containing cream, povidone iodine was run as a control. Wound healing regenerations on the skin samples were visualized by hematoxylin-eosin staining. Results: In vitro models elucidate HU-MSCM may decreasing inflammation at the beginning of wound healing, promote cell migration and angiogenesis. In addition in vivo models show that the incision length on the skin is decreasing and more smaller, HE staining describe decreasing of inflammation phase, increasing of angiogenesis, accelerate fibroplasia, and maturation phase. Conclusions: Taken together our observation indicates that HU-MSCM could promote the acceleration of skin tissue regenerations in primary wound healing process.

  7. Elastin in alcoholic liver disease. An immunohistochemical and immunoelectron microscopic study.

    Porto, L C; Chevallier, M; Guerret, S; Hartmann, D J; Grimaud, J A


    Increased elastic stained material has been described in fibrotic and cirrhotic liver processes. The aim of this work was to follow the development and distribution of elastic fibers from 48 chronic alcoholic patients. Patients were scored for fibrosis as 0, without fibrosis or minimal (n = 5); 1, incipient or early fibrosis (n = 9); 2, fibrosis or incomplete cirrhosis (n = 12); and 3, cirrhosis (n = 22). Elastica staining was performed by orcein, resorcin-fuchsin and iron hematoxylin and confirmed by immunofluorescence staining with an anti-human elastin antibody (Institut Pasteur). Electron microscopy of representative cases of each group and electron microscopy of immunolabelled elastin (n = 5) were also performed. In early alcoholic fibrosis, oxytalan fibers were pointed out in terminal hepatic veins and in Disse space. In fibrous portal extensions and cirrhotic internodular septa, oxytalan and elaunin fibers represented the major elastin components in association with the alcoholic liver fibroplasia. Immunostaining with anti-elastin Ab exhibits the same distribution as with histochemical methods in portal and septal zones. Electron microscopy confirmed abundant microfibrillar bundles between collagen fibers that mesh and are in continuity with elaunin fibers. Immunoelectron microscopy confirmed elastin deposits in the amorphous material and in association with the microfibrillar material in the portal and septal zones and disclosed elastin even in the thin strands of fibrotic tissue. In conclusion, elastogenesis, mainly represented by oxytalan and elaunin fibers, develops in alcoholic disease and takes part, with collagen deposits, in the fibrotic process. PMID:2287592

  8. Ultrastructure of oval cells in children with chronic hepatitis B, with special emphasis on the stage of liver fibrosis: The first pediatric study

    Maria Elzbieta Sobaniec-Lotowska; Joanna Maria Lotowska; Dariusz Marek Lebensztejn


    AIM: To investigate the ultrastructure of oval ceils in children with chronic hepatitis B, with special emphasis on their location in areas of collagen fibroplasia.METHODS: Morphological investigations were conducted on biopsy material obtained from 40 children,aged 3-16 years with chronic hepatitis B. The stage of fibrosis was assessed histologically using the arbitrary semiquantitative numerical scoring system proposed by Ishak et al. The material for ultrastructural investigation was fixed in glutaraldehyde and paraformaldehyde and processed for transmission-electron microscopic analysis.RESULTS: Ultrastructural examination of biopsy specimens obtained from children with chronic hepatitis B showed the presence of two types of oval cells, the hepatic progenitor cells and intermediate hepatic-like cells. These cells were present in the parenchyma and were seen most commonly in areas of intense periportal fibrosis (at least stage 2 according to Ishak et al) and in the vicinity of the limiting plate of the lobule. The activated nonparenchymal hepatic cells, i.e. transformed hepatic stellate cells and Kupffer cells were seen in close proximity to the intermediate hepatic-like cells.CONCLUSION: We found a distinct relationship between the prevalence of oval cells (hepatic progenitor cells and intermediate hepatocyte-like cells) and fibrosis stage in pediatric patients with chronic hepatitis B.

  9. Diagnostic and clinical observation on the infectious bronchitis virus strain Q1 in Italy

    Anna Toffan


    Full Text Available This paper describes the diagnostic and clinical observations of an infectious bronchitis virus (IBV variant, referred to as Q1, in clinically ill chickens in Italy. This IBV variant was described for the first time in 1998 in China. In the autumn of 2011 it caused a small-scale epidemic in non-vaccinated meat chickens in farms located in Northern Italy. The disease was characterized by increased mortality, kidney lesions and proventriculitis. Histopatological observations confirmed the nephritis and described an unusual erosive/necrotic proventriculitis with infiltration of lymphocytes, plasma cells and heterophils, as well as fibroplasia in the lamina propria. Despite these findings and the isolation of the Q1 IB virus directly from proventricular tissue, further studies are necessary to confirm the role of this IBV strain in the development of proventricular lesions. Phylogenetic analysis revealed that all the IBV isolates were very similar and probably had a common origin. The IBV Q1 variant appears to be now endemic in the North of Italy and at times it is detected in vaccinated backyard and commercial broiler farms. The importance of continuous monitoring in controlling the spread of known or emerging IBV variants is underlined.

  10. CD4(+)CD25(hi)Foxp3(+) Cells Exacerbate Bleomycin-Induced Pulmonary Fibrosis.

    Birjandi, Shirin Z; Palchevskiy, Vyacheslav; Xue, Ying Ying; Nunez, Stefanie; Kern, Rita; Weigt, S Sam; Lynch, Joseph P; Chatila, Talal A; Belperio, John A


    Idiopathic pulmonary fibrosis is a fatal lung disease with a median survival of 2 to 5 years. A decade of studies has downplayed inflammation contributing to its pathogenesis. However, these studies preceded the discovery of regulatory T cells (Tregs) and all of their functions. On the basis of human studies demonstrating Tregs can decrease graft-versus-host disease and vasculitides, there is consideration of their use to treat idiopathic pulmonary fibrosis. We hypothesized that Treg therapy would attenuate the fibroplasia involved in a preclinical murine model of pulmonary fibrosis. IL-2 complex was used in vivo to expand CD4(+)CD25(hi)Foxp3(+) cells in the lung during intratracheal bleomycin challenge; however, this unexpectedly led to an increase in lung fibrosis. More important, this increase in fibrosis was a lymphocyte-dependent process. We corroborated these results using a CD4(+)CD25(hi)Foxp3(+) cellular-based therapy. Mechanistically, we demonstrated that CD4(+)CD25(hi)Foxp3(+) cells undergo alterations during bleomycin challenge and the IL-2 complex had no effect on profibrotic (eg, transforming growth factor-β) or type 17 immune response cytokines; however, there was a marked down-regulation of the type 1 and augmentation of the type 2 immune response cytokines from the lungs. Collectively, our animal studies show that a specific lung injury can induce Treg alterations, which can augment pulmonary fibrosis. PMID:27317904

  11. Avocado/soybean unsaponifiables: a novel regulator of cutaneous wound healing, modelling and remodelling.

    Oryan, Ahmad; Mohammadalipour, Adel; Moshiri, Ali; Tabandeh, Mohammad R


    We investigated the effects of avocado/soybean unsaponifiables (ASU) on the healing response of cutaneous wound defect in rats. Sixty male rats were randomly divided into three groups including control, vehicle and treatment (n = 20 in each group). A 2 × 2 cm(2) wound defect was made on the dorsum. The control, vehicle and treatment groups were treated daily with topical application of saline, cream and cream/ASU for 10 days, respectively. The wounds were monitored daily. The animals were euthanised at 10, 20 and 30 days post injury (D). The dry matter, hydroxyproline, collagen, n-acetyl glucosamine (NAGLA) and n-acetyl galactosamine (NAGAA) contents of the skin samples were measured and the histopathological and biomechanical characteristics of the samples were investigated. Statistics of P < 0·05 was considered significant. Treatment significantly increased tissue glycosaminoglycans and collagen contents at various stages of wound healing compared to controls. Treatment modulated inflammation, improved fibroplasia and produced high amounts of scar tissue at short term. At long term, treatment reduced the scar tissue size and increased the quality and rate of wound contraction and reepithelisation compared to controls. The treated lesions were more cosmetically pleasing and had significantly higher biomechanical characteristics than controls. ASU was effective in rat wound healing. PMID:24321012

  12. A influência da calcitonina sintética de salmão na cicatrização cutânea de ratos Influence of the synthetic salmon calcitonin in cutaneous wound healing of the rats

    José Neiva Eulálio


    Full Text Available OBJETIVO: O presente trabalho tem como objetivo investigar a influência da calcitonina sintética do salmão no processo cicatricial da pele da região dorsal em ratos, analisando parâmetros bioquímicos, biomecânicos e histológicos, e suas possíveis interrelações. MÉTODO: Setenta e dois (72 ratos machos adultos foram submetidos, sob anestesia geral inalatória a uma incisão linear na pele que foi, subseqüentemente, suturada. Metade dos ratos (grupo teste foi tratada no pós-operatório com calcitonina sintética do salmão, e a outra metade, sem tratamento, constituiu o grupo controle. Os animais, em pares de nove ratos cada (teste e controle, foram sacrificados no 5º, 10º, 15º e 20º dias pós-operatório para a remoção dos fragmentos de pele com cicatriz para as análises bioquímicas, biomecânicas e histológicas. Utilizou-se o teste "t" de Student para a comparação de médias de amostras independentes e o teste de correlação de Pearson para verificar o grau de associação entre as médias das variáveis. RESULTADOS: A calcitonina sintética de salmão acelerou o processo cicatricial da pele, mas não de maneira linear e constante. Em comparação com os animais não-tratados, houve aumento significativo tanto do conteúdo de hidroxiprolina nas fases de proliferação inicial e tardia da cicatrização, quanto da carga máxima de ruptura na fase de proliferação tardia. Os resultados histológicos corroboram os resultados bioquímicos e biomecânicos, sugerindo uma correlação entre conteúdo de colágeno, resistência à tração e histologia da cicatriz. CONCLUSÃO: A calcitonina sintética de salmão acelerou o processo cicatricial da pele, modificando significativamente o conteúdo de colágeno e a carga máxima de ruptura, mas não de maneira linear e constante.BACKGROUND: The aim of the present study was to investigate the influence of synthetic salmon calcitonin on wound healing in dorsal skin of rats

  13. Estudo histológico comparativo de implantes (ímãs em órbitas de coelhas Comparative histologic study of implants (magnets in orbits of female rabbits

    Elcio Roque Kleinpaul


    Full Text Available OBJETIVOS: Avaliação microscópica dos sinais da reação inflamatória causada pela fixação de implante orbitário (ímã, recoberto por ouro. MÉTODOS: Estudo experimental em 54 coelhas adultas, divididas em três grupos de 18, com 3 subgrupos de 6, avaliação com uma, três e seis semanas de pós-operatório. Fixação, no rebordo inferior da órbita, de implante (ímã e ouro por meio de cola, parafuso ou fio inabsorvível (Mersilene 5.0®. As órbitas contralaterais (sem implante compuseram o grupo-controle. RESULTADOS: Sinais histológicos de reação inflamatória à fixação orbitária do implante foram observados, com cianoacrilato, parafuso, ou sutura inabsorvível (Mersilene 5.0®, até a sexta semana pós-operatória, com predomínio do processo inflamatório crônico e fibrótico cicatricial, sem demonstrar diferenças estatisticamente significativas na maior parte dos casos. CONCLUSÃO: O exame histopatológico demonstrou, na maior parte das órbitas com e sem implante, presença de reação inflamatória aguda e crônica até a sexta semana pós-operatória, com predomínio de processo inflamatório crônico e fibrótico cicatricial, sem demonstrar, na maior parte dos casos, diferenças estatisticamente significante entre os grupos experimental e controle.PURPOSE: To evaluate microscopically the signs of inflammatory reaction due to the fixation of an orbitary implant (magnet, covered by gold. METHODS: An experimental study was performed in 54 adult female rabbits, divided into 3 groups of eighteen with 3 subgroups of 6; an evaluation at 1, 3 and 6 postoperative weeks was performed. An implant (magnet, covered by gold was fixed in the inferior orbital rim using biologic glue, screw or unabsorbable suture (Mersilene 5.0®. The opposite orbits (without implants represented the control group. RESULTS: Microscopic signs of inflammatory reaction due to orbitary fixation of the implant were observed with the use of the three

  14. Effects of preoperative pelvic irradiation on colonic anastomosis healing: an experimental study in rats Efeito da radioterapia pélvica pré-operatória na cicatrização de anastomoses colônicas: estudo experimental em ratos

    Alexandre Franca


    Full Text Available PURPOSE: Colorectal anastomosis is a constant worry-issue among surgeons because of high rates of complications, specially the dehiscence. The preoperative irradiation on cancer surgeries might interfere in the healing process, leading to an unfavorable outcome. METHODS: In the present study, two groups of rats were irradiated previously to a colorectal anastomosis surgery, with intervals of 4 and 8 weeks between the procedures. Seven days after the surgery, healing process was evaluated for dehiscence presence and histologic inflammatory characteristics. Also, levels of hydroxyproline, metalloproteinases and vascular endothelial growth factor were measured. RESULTS: Our results showed a higher incidence of dehiscences on the animals submitted to irradiation, compared to controls, with a reduced inflammatory activity in the healing tissue. DISCUSSION: Comparing both irradiated groups, those irradiated 8 weeks before surgery showed higher levels of hydroxyproline and metalloproteinases, indicating higher efficiency of the healing process. In conclusion, preoperative irradiation interferes with intestinal anastomosis healing and a larger time interval between both procedures is safer in terms of the healing quality.INTRODUÇÃO: As anastomoses colorretais são motivos constante de preocupação por parte dos cirurgiões, em virtude do alto índice de complicações, principalmente as deiscências. O uso da radioterapia previamente à cirurgia, nos casos de doença neoplásica, pode interferir no processo cicatricial das anastomoses, e levar a uma evolução desfavorável. MÉTODOS: Os autores estudaram dois grupos de ratos, submetidos a radioterapia e à confecção de uma anastomose no cólon, com intervalo de 04 e de 08 semanas entre os dois procedimentos, comparando com um grupo controle. Após 07 dias da cirurgia, estudaram-se vários aspectos do processo cicatricial: presença de deiscência, características inflamatórias do tecido, dosagem

  15. Uso da cola n-butil cianoacrilato na sulcoplastia de recessão em coelhos: avaliação histológica Use of n-butyl cyanoacrylate glue in the recession sulcoplasty in rabbits: histologic study

    Fernanda Carpi dos Santos


    Full Text Available O trabalho teve por objetivos analisar a cola composta por n-butil cianoacrilato, como um auxiliar na fixação da cunha óssea no procedimento da sulcoplastia de recessão e sua interferência no processo cicatricial. Foram utilizados nove coelhos da raça Norfolk, machos, com peso inicial entre 3,0 e 3,5 kg. Após anestesia epidural, realizou-se acesso parapatelar medial e seccionou-se uma cunha ósseo-cartilaginosa em formato de V no sulco da tróclea. No joelho direito, antes da colocação da cunha em seu leito, foram aplicadas duas gotas da cola, ao passo que, no joelho esquerdo, considerado controle, a cunha foi apenas recolocada. Para realização da técnica de exame anatomopatológico, foram submetidos à eutanásia três animais aos cinco, 15 e 30 dias de pós-operatório. Na avaliação macroscópica, notou-se, em ambos os joelhos, a estabilização da cunha ósseo-cartilaginosa em seu leito receptor nos diferentes momentos de observação. Pelo exame histológico foi possível concluir que a cola não induziu o aparecimento de processo inflamatório acentuado e reação do tipo corpo estranho, mas interferiu negativamente, retardando o processo cicatricial.The aim of this study was to analyze the glue composed by n-butyl cyanoacrylate, as an auxiliary in the fixation of the bone wedge at the recession sulcoplasty procedure and the interference in the healing. Nine male Norfolk rabbits, initially weighing from 3 to 3.5 kg were used. After epidural anesthesia, a medial parapatellar approach was used and a V-shaped osteochondral wedge was removed from the trochlea. In the right stifle joint, before the osteochondral wedge replacement two glue drops were used. In the left stifle joint, considered as control, the osteochondral wedge was only replaced. Three animals were submitted to euthanasia at five, 15 and 30 days of postoperative period. Macroscopic evaluation showed that in both stifles the osteochondral wedge was stabilized on the

  16. Control of the skin scarring response

    Lydia M. Ferreira


    Full Text Available There comes a time when the understanding of the cutaneous healing process becomes essential due to the need for a precocious tissue repair to reduce the physical, social, and psychological morbidity. Advances in the knowledge on the control of interaction among cells, matrix and growth factors will provide more information on the Regenerative Medicine, an emerging area of research in medical bioengineering. However, considering the dynamism and complexity of the cutaneous healing response, it is fundamental to understand the control mechanism exerted by the interaction and synergism of both systems, cutaneous nervous and central nervous, via hypothalamus hypophysis-adrenal axis, a relevant subject, but hardly ever explored. The present study reviews the neuro-immune-endocrine physiology of the skin responsible for its multiple functions and the extreme disturbances of the healing process, like the excess and deficiency of the extracellular matrix deposition.Aproxima-se uma época na qual é fundamental a compreensão do processo cicatricial cutâneo frente à necessidade da restauração tecidual precoce, visando a diminuição das morbidades física, social e psicológica. O avanço no conhecimento acerca do controle das interações entre as células, a matriz e os fatores de crescimento dará maiores informações à Medicina Regenerativa, área de pesquisa emergente da bioengenharia médica. Entretanto, diante do dinamismo e complexidade da resposta cicatricial cutânea torna-se indispensável o entendimento do mecanismo de controle exercido pela interação e sinergismo do sistema nervoso cutâneo e o sistema nervoso central, via eixo hipotálamo-hipófise-adrenal, tema relevante, porém, pouco abordado. O presente estudo revisa a fisiologia neuro-imuno-endócrina da pele, responsável por suas múltiplas funções, e os distúrbios extremos do processocicatricial, como o excesso e deficiência de deposição da matriz extracelular.

  17. Ultra-som terapêutico na cicatrização tecidual Therapeutic ultrasound in the tissue healing

    Débora Cristina Olsson


    Full Text Available Visando a minimizar o período de tratamento cicatricial em diversos tecidos, buscam-se alternativas à terapia convencional que colaborem com o processo reparativo criando um microambiente ideal para sua ocorrência. Dentre os inúmeros benefícios das técnicas aplicáveis à medicina regenerativa, o ultra-som terapêutico (UST é prática adjuvante cada vez mais freqüente. Os modelos experimentais de terapia sonora em animais têm propiciado informações relevantes para o tratamento de vários tipos de lesões; todavia, apesar de serem muito utilizados, ainda existem controvérsias em relação aos seus potenciaia biológicos de acordo com a modalidade e a dosimetria indicadas para cada caso. Os efeitos biofísicos do ultra-som (US sobre o reparo tecidual são pouco compreendidos, sendo seu uso muitas vezes negligenciado ou fundamentado na experiência prática, o que resulta em procedimentos errôneos. O objetivo desta revisão bibliográfica é apresentar informações sobre o UST, relatar a existência de riscos de danos celulares decorrentes da inadequada aplicação e revelar a importância de seus mecanismos de ação nos tecidos, tanto no sentido físico, como nos seus aspectos biológicos.Aiming to minimize the cicatricial treatment period, alternatives have been searched to conventional therapy, among which the therapeutic ultrasound is a practice more and more frequent. However, despite of being used in the treatment of various lesions types, there are still controversies related to its real therapeutical value and the indicated dosimetry for each case. The ultrasound effect on the tissue repair is little understood, and its use is seldom based on the practical experience, what sometimes results in wrong procedures. This review has the objective to show the therapeutic ultrasound information and to reveal the importance of its mechanisms in the tissues in the physical and biological aspects.

  18. Barreira cutânea na dermatite atópica Skin barrier in atopic dermatitis

    Flavia Alvim Sant'Anna Addor


    Full Text Available O estudo da barreira cutânea e de suas propriedades ganhou impulso a partir da década de 60, com estudos que apontaram sua resistência de forma isolada e suas propriedades com relação à permeação cutânea. Paralelamente, a descrição dos corpos de Odland auxiliou a compreensão da manutenção da estabilidade da camada córnea. O modelo brick & mortar, em que os corneócitos são os tijolos e o cimento são os lipídeos intercelulares, é o mais aceito, até o momento. Atualmente, há evidências consistentes de que o estrato córneo é uma estrutura metabolicamente ativa e exerce funções adaptativas. A barreira cutânea também tem um papel na resposta inflamatória, com ativação de melanócitos, angiogênese e fibroplasia, cuja intensidade depende, basicamente, da intensidade da agressão. As anormalidades da barreira cutânea da dermatite atópica são clinicamente observáveis pela presença de pele seca, achado muito frequente e significativo, que constitui parâmetro iagnóstico e de acompanhamento. O grau de hidratação da camada córnea, assim como a perda de água transepidérmica (transepidermal water loss - TEWL, estão relacionados com o grau de dano à barreira, constituindo parâmetros biofísicos que permitem acompanhar os pacientes de maneira não invasiva e com maior grau de sensibilidade.Research about the skin barrier and its properties has increased significantly since the 60s, with studies that indicated its resistance when isolated, as well as its particularities in relation to skin permeability. At the same time, description of Odland bodies helped to understand how stratum corneum stability is maintained. The “brick and mortarâ€� model is the most accepted so far. In this analogy, the corneocytes are the bricks and the intercellular lipids are the mortar. Currently, there is concrete evidence that the stratum corneum is an active metabolic structure that holds adaptive functions, interacting

  19. 枸杞多糖对小鼠移植性肝癌抑制作用的实验研究%Inhibition of Lycium barbarum polysaccharide on transplanted liver cancer in mice

    张鸣号; 王秀玉; 王秀梅; 刘青; 杨美玲


    Objective To explore the inhibition of Lycium barbarum polysaccharide (LBP) on transplanted liver cancer in mice. Methods The transplanted liver cancer model was established with hypodermic inoculation. Ninety-six mice were randomly divided into six groups: control group, model group, LBP high-, middle-, and low-dose (20, 10, and 5 mg/kg) groups, and cyclophosphamide (20 mg/kg) group. The effects of LBP on mortality, gross tumor volume, inhibitory rate of tumor growth, tumorous cellular density, karyokinesis, degree of tumor necrosis, lymphocyte infiltration, and fibroplasia in interstitial substance in tumor-bearing mice were observed. Results LBP with high, middle, and low doses could obviously depress the death rate of tumor-bearing mice (P < 0.05) and diminish gross tumor volume and weight (P < 0.05). The inhibitory rates of tumor growth were 42.23%, 25.10%, and 9.16%. LBP with high and middle doses could degrade the tumorous cellular density (P < 0.05), decrease the karyokinesis (P < 0.05), and increase the degree of tumor necrosis, lymphocyte infiltration, and fibroplasia in interstitial substance in mice (P < 0.05). Conclusion LBP could produce the inhibition on transplanted liver cancer in mice.%目的 探讨枸杞多糖对小鼠移植性肝癌的抑制作用.方法 采用皮下接种法制备小鼠移植性肝癌(H22肝癌细胞株)模型,随机将96只小鼠分成对照组、模型组、枸杞多糖高、中、低剂量(20、10、5 mg/kg)组、环磷酰胺(20 mg/kg)组.观察枸杞多糖对荷瘤小鼠死亡率、肿瘤体积、肿瘤质量及肿瘤细胞密度、核分裂像、肿瘤坏死程度及间质淋巴细胞浸润、纤维组织增生情况的影响.结果 20、10、5 mg/kg的枸杞多糖能明显降低移植性肝癌小鼠的死亡率(P<0.05),减小肿瘤细胞的体积和质量(P<0.05),抑瘤率分别为42.23%、25.10%和9.16%;同时20、10 mg/kg的枸杞多糖能不同程度地降低肿瘤细胞密度(P<0.05),减少肿瘤

  20. Wound healing in the jugal mucosa of rats with a cold blade scalpel and an ultrasonic harmonic scalpel Cicatrização de feridas realizadas na mucosa jugal de ratos com bisturi com lâmina fria e com bisturi harmônico ultrassônico

    Elen Carolina David João De Masi


    Full Text Available Ultrasound harmonic scalpel has been recently introduced in otorhinolaryngological procedures. AIM: to assess macro and microscopic evolution of the healing process of wounds created in the jugal mucosa of rats by the use of ultrasound scalpel. METHOD: we used 30 Wistar rats in which we made mucosal incisions on the right jugal mucosa with the ultrasound harmonic scalpel (USHS and on the left side with the cold blade scalpel (CBS. Macroscopic and microscopic evaluations were carried out on the third, seventh and fourteenth days of postoperative. For the microscopic evaluation we used HE to asses the inflammatory process and the Sirius Red approach for collagens type I and III. Anti-CD 3 antibodies and anti-factor VIII assessed the concentration of T-lymphocytes and neovessels. RESULTS: the USHS caused greater cell damage with reepitelization delay. Microscopy showed more intense inflammatory reactions and a loss in collagen build up, delay in scar maturation and a greater vessel neoformation. CONCLUSION: USHS brings about a greater lesion in the incision area; delayed regeneration; promotes greater inflammatory process and angiogenic activity; delays in fibroplasia and scar tissue maturation on the rats' jugal mucosa when compared to cold blade scalpel.O bisturi harmônico ultrassônico foi introduzido recentemente em operações otorrinolaringológicas. ObJETIVO: Avaliar a evolução macro e microscópica do processo de cicatrização de feridas realizadas na mucosa jugal de ratas com uso de bisturi ultrassônico. MÉTODO: Foram utilizadas 30 ratas Wistar com incisões na mucosa, no lado direito com bisturi harmônico ultrassônico (BHU e à esquerda com bisturi com lâmina fria (BLF. As avaliações macroscópicas e microscópicas foram realizadas no terceiro, sétimo e décimo quarto dias pós-operatório. Na análise microscópica utilizou-se coloração HE para avaliar o processo inflamatório e a técnica de Sirius red para os colágenos I

  1. Pengaruh Cairan Cultur Filtrate Fibroblast (CFF Terhadap Penyembuhan Luka; Penelitian eksperimental pada Rattus Norvegicus Galur Wistar

    Oky Masir


    Full Text Available AbstrakLatar belakang:Metode penyembuhan luka telah mengalami perkembangan, baik berupa suatu produk atau stimulan terhadap proses biologis tubuh dalam menkompensasi luka. Fibroblas merupakan salah satu komponen penyembuhan yang berperan penting dalam proses fibroplasia. Culture Filtrate Fibroblast (CFF merupakan hasil kultur fibroblas yang akan dibuktikan efeknya terhadap proses percepatan penyembuhan luka pada penelitian ini. Metode. Penelitian ini menggunakan desain eksperimental dengan metode post test only control group design dan rancangan acak kelompok (RAK dengan menggunakan tikus putih wistar. Hewan coba dibagi menjadi 4 kelompok, yaitu 2 kelompok perlakuan yang diberikan CFF ke area eksisi luka dan kelompok kontrol yang diberikan larutan NaCl 0,9% ke area eksisi luka. Data diolah dengan SPSS 16.0. Data Kategori dianalisa dengan Chi-squared dan data numerik dengan Independent T-test. Hasil. Dari tingkat penyembuhan tidak ditemukan perbedaan pada kedua kelompok, namun perubahan restriksi jaringan lebih besar pada kelompok perlakuan. Pada skor pembentukan kolagen, derajat epitelisasi serta jumlah pembentukan pembuluh darah baru pada hari ke-3 tidak ditemukan perbedaan antara kedua kelompok. Namun pada pengamatan hari ke-7 memperlihatkan pembentukan kolagen, derajat epitelisasi serta jumlah pembentukan pembuluh darah baru lebih banyak pada kelompok perlakuan. Pada fibrosis hari ke-3 dan hari ke-7 memperlihatkan terjadinya fibrosis lebih banyak pada kelompok perlakuan dibanding kontrol. Pada pengamatan terjadinya infeksi hari ke-3 memperlihatkan infeksi lebih sedikit pada kelompok perlakuan dan terjadinya infeksi sama pada hari ke-7. Kesimpulan. CFF memberikan tingkat penyembuhan luka yang lebih baik dibanding NaCl.Kata kunci: CFF, NaCl 0,9 %, tingkat penyembuhan luka.Abstract Background: Wound healing methods have been developed, either a product or a stimulant to the body's biological processes in wound compensation. Fibroblasts is one

  2. Componentes ricos em plaquetas na reparação de afecções tendo-ligamentosas e osteo-articulares em animais Components rich in platelets used in wound healing tendon, ligaments and osteo-articular diseases of animals

    Leandro Maia


    Full Text Available O plasma rico em plaquetas e o concentrado de plaquetas são fontes de diversos fatores de crescimento, com grande potencial terapêutico. Uma vez liberados dos grânulos alfa das plaquetas ativadas, esses fatores atuarão no sítio da lesão, estimulando a quimiotaxia, fibroplasia e angiogênese, melhorando assim a reparação tecidual. Embora esses componentes ricos em plaquetas sejam de fácil obtenção e de eficácia comprovada na medicina humana e odontologia, a utilização desses componentes na medicina veterinária é relativamente recente, necessitando ainda de estudos controlados. Neste artigo, os aspectos morfológicos das plaquetas, a ação dos fatores de crescimento e a utilização de componentes ricos em plaquetas na reparação tecidual de estruturas tendo-ligamentosas e osteo-articulares são revisados.Platelet-rich plasma and platelet concentrates are involved in growing factors. Both have great therapeutic potential. When the alpha-granules are released by the active platelet, they act in the lesion site stimulating the chemotaxis, the fiberplasia and the angiogenesis. They improve the regeneration of the tissue. Although these rich platelet components are easy to obtain and efficiently to prove by medicine and dentistry, their use on vet medicine is recent. So, much more control studies need to be done. This article reviews morphological aspects of platelets, action of growing factors and use of components rich in platelet in wound healing tendons, ligaments and osteo-articular structures.

  3. Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats.

    Ibrahim, Zein Shaban; Alkafafy, Mohamed Elsayed; Ahmed, Mohamed Mohamed; Soliman, Mohamed Mohamed


    The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male Wistar rats were divided into four groups of six rats each. DM was induced by a single intraperitoneal injection of streptozotocin 60 mg/kg body weight. DM rats were treated with or without NC in the absence or presence of curcumin for 8 weeks. As compared with the controls, DM rats exhibited reduced serum levels of high density lipoprotein, superoxide dismutase and glutathione peroxidase, and decreased renal mRNA expression levels of synaptopodin, connexin 43 and erythropoietin (EPO), which were further suppressed by NC and restored to normal levels by curcumin treatment. Additionally, DM rats exhibited increases in their lipid profiles (cholesterol, triacylglycerol and phospholipids), oxidative markers (malondialdehyde, γ‑glutamyltranspeptidase and nitric oxide), kidney function markers (urea and creatinine) and the mRNA expression levels of vimentin, desmin, SREBP‑1, iNOS and TGF‑β1. These effects were further enhanced by NC, but counteracted by curcumin treatment. Kidneys from DM rats displayed glomerular hypertrophy, sclerosis and tubulo‑interstitial changes represented by tubular lipid deposition, interstitial mononuclear cell infiltration and fibroplasia. Pancreatic islets exhibited cellular vacuolation, morphological irregularity and damaged or reduced in size β‑cells. These renal and pancreatic changes became more severe following NC treatment and were ameliorated by curcumin. Therefore, NC‑induced DN progression may predominantly operate by increasing oxidative stress, reducing the levels of antioxidants, suppressing EPO levels, and causing perturbations to gap junction and podocyte structure. Curcumin may ameliorate the damaging effects of DM and NC on the kidney through normalization

  4. Preparation of fucoidan-chitosan hydrogel and its application as burn healing accelerator on rabbits.

    Sezer, Ali Demir; Cevher, Erdal; Hatipoğlu, Fatih; Oğurtan, Zeki; Baş, Ahmet Levent; Akbuğa, Jülide


    Treatment of dermal wounds with macromolecular agents such as natural polymers is one of the research areas of the biomaterial science. Fucoidan is a sulphated polysaccharide which is commonly obtained from seaweeds. The great number of studies on the different pharmacological properties of fucoidan is present, but there is limited information about using of fucoidan in the treatment of dermal burns. The aim of this study was to prepare fucoidan-chitosan hydrogels and to investigate their treatment efficiency on dermal burns. Hydrogels were prepared by swelling the polymers in acidic solution and their swelling, mechanical (hardness, cohesiveness and adhesiveness) and bioadhesive properties were investigated. The viscosity and water absorption capacity of formulations increased with increase in the polymer concentration. In contrast to the cohesiveness results, the adhesiveness of hydrogels increased with the polymer concentration. The bioadhesion was changed between 0.012-0.142 mJ x cm(-2) and enhanced with addition of fucoidan into gel formulations. It was formed dermal burns on seven adult male New Zealand white rabbits and the optimum gel formulation applied on the wounds. Control and treatment group biopsy samples were taken on days 7, 14 and 21 and each burn wound site was evaluated histopathologically. No edema was seen in tested groups except control after 3 d treatment. After 7 d treatment, fibroplasia and scar were fixed on wounds treated with fucoidan-chitosan gel and fucoidan solution. The best regeneration on dermal papillary formation and the fastest closure of the wounds were observed in fucoidan-chitosan hydrogels after 14 d treatment. PMID:19043221

  5. In Vivo Evaluation of the Effects of Gravitational Force (+Gz) on Over-the-Wire Stainless Steel Greenfield Inferior VenaCava Filter in Swine

    This study was done to determine the effect of exposure to gravitational force (acceleration stress) on invivo over-the-wire stainless steel Greenfield inferior vena cavafilters. Fifteen pigs underwent venous cut down and placement of a stainless steel Greenfield filter. A 4-week observation period simulated realistic convalescence and allowed sufficient time for epithelialization. Ten pigs were exposed to acceleration stress in a centrifuge (3G run for 15 sec followed by rest until return to baseline heart rate, then a 9G run for 15 sec), with inertial loading in ahead-to-tail direction (+Gz). Fluoroscopy during acceleration stress allowed assessment for filter migration. Five pigs were not exposed to acceleration stress. AP and lateral abdominal radiographs were obtained at post-filter placement, convalescence, and centrifuge exposure to determine the position and integrity of the filter. All 15 IVCs were resected and evaluated for gross or histological injury to the vessel wall. IVC filter placement was technically successful in all 15 pigs.Radiographic measurements were limited secondary to differences in pig positioning. Fluoroscopy showed no filter migration. All filters were securely attached to the vena cava by the hooks without gross evidence of perforation or hemorrhage. There were varying degrees of fibroplasia involving the hooks and tip of the filters in both the control and experimental groups. Histologically, there was evidence of prior hemorrhage at the level of the hooks, which was similar between the control and experimental groups. It is concluded that Greenfield filter position and vena caval integrity at the implantation site is unaffected by high acceleration stress

  6. Morphological analysis of second-intention wound healing in rats submitted to 16 J/cm 2 λ 660-nm laser irradiation

    Gonzaga Ribeiro Maria


    Full Text Available Background and Objectives : Low-level laser therapy (LLLT has been extensively applied to improve wound healing due to some biostimulatory properties presented by laser arrays apparently able to accelerate the cicatricial repair of soft tissue injuries. However, many controversial results have been reported in the literature, probably as a result of the wide sort of different protocols of photobiomodulation employed in those experiments. The goal of this study was to investigate the effect of a low-dose protocol of LLT on the intensity of the inflammatory response and the pattern of collagen fibers′ deposition during second-intention wound healing in rodents. Materials and Methods : Standard-sized wounds were carried out in the back of 24 male rats. Half of them underwent LLLT treatment (16 J/cm 2 at 660 nm delivered for 7 days. Eight and 14 days after the wounds were performed, the repairing area was removed and stained in HE and Masson′s trichrome, and the inflammatory response, epithelization, and collagen fiber depositions were evaluated. Results : We found that LLLT was able to slightly reduce the intensity of the inflammatory reaction as well as to enhance substantially the epithelization process at both 8 th and 14 th days. In addition, it also appeared to stimulate the deposition of collagen fibers at the final stages of wound healing. Conclusions : The LLLT protocol tested in this study resulted in some improvements in second-intention wound healing in rodents.

  7. Clinical And Morphological Androgenic Status Characteristics At Children Suffering From Hypospadias And Its Influence On Results Of Surgical Correction

    F.K. Napolnikov


    Full Text Available The goal of this article is to estimate the androgenic status and analyze its influence on the surgical treatment of hypospadias. From 2000 till 2008 there were 209 children under treatment, whose age varied from 8 months till 15 years old (average age — 4,5+ 1,5. 49 patients were subjected to clinical, humoral and morphological study. Preoperational preparation was carried out by testosterone medications. The comparison group consisted of 10 boys with cicatricial phimosis. The patients with the medium and back forms of hypospadias suffered from the androgenic deficit characterized by proximal level of meatus ectopia, diminution of penis length and prostate volume, decrease of blood vessels in deep layers of penis skin. The testosterone pre-operational medication of patients makes possible to improve the results of surgical correction due to blood supply of plastic material. On the basis of recieved data logistically regressive model has been worked out and the prognosis of results has been estimated

  8. O autocuidado para o tratamento de úlcera de perna falciforme: orientações de enfermagem

    Amanda Martins


    Full Text Available O objetivo deste estudo foi verificar a eficácia do Programa do Autocuidado, pela progressão do processo cicatricial de úlcera da perna falciforme. Trata-se de um estudo longitudinal com abordagem quantitativa realizado com 40 clientes no setor de curativos de uma instituição de saúde pública, especializada no tratamento hematológico, situada no Rio de Janeiro. Para a coleta dos dados foi utilizado um instrumento aplicado no momento das consultas de enfermagem. Os dados foram analisados com o auxílio dos modelos de Cox, regressão logística e árvores de classificação. Verificou-se a eficácia do Programa do Autocuidado em função da cicatrização total das ulcerações de 23 clientes, e observou-se que úlceras agudas com um tempo de manifestação até 60 meses têm a probabilidade de cura de 95%. A partir da adesão dos clientes e dos resultados alcançados, o programa firmou-se como modalidade terapêutica, sendo incorporado como programa institucional.

  9. Experimental model for establishment of hypoxia in 75 cm² culture flasks Modelo experimental para o estabelecimento de hipóxia em balões de cultura de 75 cm²

    Sidney Mamoru Keira


    Full Text Available In Plastic Surgery, cell culture represents the perspective of studying cellular mechanisms that guide the healing process of several tissues. Some steps of the healing process depend on physical factors as the tissular partial pressure of O2. In cell culture, it is possible to submit cells to hypoxic enviroment. The present study reports an alternative method at low cost for the establishment of hypoxic environment in cell culture flasks.A cultura de células, na Cirurgia Plástica, representa uma perspective para o estudo dos mecanismos celulares que norteiam o processo cicatricial de diversos tecidos. Algumas etapas do processo de cicatrização dependem de fatores físicos como a pressão parcial de O2. Em uma cultura de células, é possível submeter células a um ambiente hipóxico. O presente estudo relata um método alternativo de baixo custo para o estabelecimento de um ambiente hipóxico em frascos de cultura de células.

  10. Scintillation Scanning in Studying Regional Arterial Flow of the Extremities in Vascular Disorders of Connective Tissue

    The authors present preliminary results of a study involving the injection of 131I-labelled macroaggregated albumin (MAA) into the arterial trunk of the extremity, followed by scanning of the limb in question, for the purpose of investigating variations in regional articular flow in the proximal portions of the extremities of patients suffering from vascular disorders of connective tissue. In patients suffering from acute, active polymyositis, scanning shows an extensive build up of radioactivity in the proximal muscular masses of the limb, due to their hyperaemia. In the case of chronic slightly active polymyositis it shows an appreciable reduction in the accumulation of radioactivity in the said regions, due to cicatricial factors and to fibrosis. In cases of morphoea, an extensive build up of radioactivity, due to hyperaemia, was observed in the areas underlying the sclerotic patch. In nodular polyarthritis, it was possible to identify the zones of vasculitis, which appeared in the scintigram as true accumulations of radioactivity. The authors have come to the conclusion that this method is rapid, simple and devoid of serious inconvenience to the patient. They believe that it will be possible to make use of it in future for the study of vascular, muscular and connective tissue disorders. However, its soundness from the practical point of view remains to be established. (author)

  11. Clinicoepidemiological Observational Study of Acquired Alopecias in Females Correlating with Anemia and Thyroid Function

    Kirti Deo


    Full Text Available Alopecia can either be inherited or acquired; the latter, more common, can be diffuse, patterned, and focal, each having cicatricial and noncicatricial forms. This observational study of 135 cases in a semiurban Indian population aimed to detect the prevalence of various forms of acquired alopecia in females and correlate the same with levels of hemoglobin, serum ferritin, triiodothyronine, thyroxin, and thyroid stimulating hormone. The majority (84, 62.2% of our cases of alopecia had telogen effluvium followed by female pattern alopecia (32, 23.7%. Stress (86, 63.7%, topical application of chemicals (72, 53.3%, systemic medications for concurrent illnesses (62, 5%, and pregnancy (14, 10.3% were the common exacerbating factors. Neither low hemoglobin (12 μg/L. Though lack of vitamin B12 testing was a limitation of our study, its deficiency could be the probable cause of iron deficiency as the majority (58, 64.4% of these cases, as indeed majority (89, 65.4% of our study population, were vegetarians. Thyroid disorders (23, 17%, including 9 newly diagnosed were not of significance statistically.

  12. Nasal meatus plasty: a contribution to plastic reconstruction of the nasal valve during midfacial degloving

    Lorenz, Kai J.


    Full Text Available Midfacial degloving is a proven method for easily accessing the midface, the nasal pyramid, the maxillary and ethmoidal sinuses, the orbits, as well as the anterior skull base. Indications for this method of access mainly include tumour resections in the area of the midface, the septum, the maxillary sinus, the paranasal to the sphenoidal sinus as well as the clivus. In addition, this method of access allows for the exposure of the bony structures of the midface in the event of extensive fractures.In general, this method of access combines an incision in the oral vestibule and circular incisions in the nasal vestibule area in order to release the nasal pyramid.After removing the facial wall of the maxillary sinus, extensive exposure of the surgical site is possible.One disadvantage of this method of access is the difficult reconstruction of the nasal valve area, which often leads to cicatricial stenoses and difficulties with breathing through the nose. Furthermore, wound healing problems and osteoradionecrosis in the area of the lateral margin of the anterior nasal aperture after replantation of the facial wall of the maxillary sinus have been described, because in this area sufficient soft tissue coverage cannot be ensured when a conventional technique is used.We describe a soft tissue flap pedicled in the cranial and caudal directions in the nasal valve area which makes both the reconstruction of the nasal vestibule and sufficient soft tissue coverage of the anterior nasal aperture possible.

  13. JAW CYSTS AND GUIDED BONE REGENERATION (a late complication after enucleation

    Hristina Lalabonova


    Full Text Available Maxillary jaw bone possesses a high regenerative capacity. Yet sometimes the defects enucleation of jaw cysts leaves may regenerate only partially or not at all. For this reason some researchers advise treatment of the residual cavities after cystectomy using bone regeneration stimulation methods. We report a case of an atypical complication after enucleation of a maxillary cyst manifesting itself eight years after the initial treatment. The symptoms the patient reported were at first periodic sweating on the left sides of face and head. This was followed by a piercing pain in the left palpebral fissure radiating to the middle of the palate and felt in the left cheekbone, left eye and left supraorbital ridge. The patient has a history of maxillary cysts recurring three times and of three operations she had 20, 12 and 8 years previously. The multiple recurrences of the cysts after their enucleation indicates poor regenerative capacity of the body which resulted in the formation of cicatricial tissue. It is most probably this tissue that was responsible for the disruption of the nerve conduction capacity which can account for the reported symptoms. We filled the cavity with bone graft material which boosted the bone structure regeneration. Although maxillary jaws possess high regenerative capacity we advise the use of guided bone regeneration in cases of large bone defects that usually occur after enucleation of jaw cysts.

  14. Short Term Outcome of Anterior Lamellar Reposition in Treating Trachomatous Trichiasis

    Rania A. Ahmed


    Full Text Available Purpose. To evaluate the outcome of anterior lamellar reposition (ALR in treating trachomatous trichiasis. Methods. Patients with trachomatous trichiasis or entropion with short tarsus were treated by ALR between February 2009 and November 2013. This included splitting of the lid margin behind the aberrant lash line to separate the lid lamellae. The anterior lamella was recessed and fixated using 4/0 silk sutures. The extra lashes and their routes were excised. Sutures were removed by the 3rd week and patients completed 6 months of follow-up. Recurrence of ≤5 lashes was treated by electrolysis. Results. The study included 752 eyelids (445 patients; 58.4% females, 41.6% males, mean age 53.2 ± 6.9 y. 179 (25.1% lids had entropion while 287 (64.5% patients had corneal affection. By the third week, 2.66% lid had trichiasis while 30.8% had no rubbing lashes. By the 6th month, 14.9% of lids showed recurrence while 66.1% were completely cured (CI = 0.63–0.69 and 19% had partial success (CI = 0.16–0.21. Abnormal lid appearance persisted in 2.66% and 12.9% required another surgery. Conclusion. ALR is a good option for treating trachomatous trichiasis especially without cicatricial entropion. Excision of dysplastic lashes is thought to augment the surgical outcome.

  15. Hyperbaric oxygen therapy in the surgical approach to an irradiated patient

    Antônio Figueiredo Caubi


    Full Text Available Cancer is the terminology applied to a set of more than one hundred diseases that have disorderly cell growth in common. The treatmentof cancer can be performed by surgery, radiotherapy, chemotherapy, or bone marrow transplant. In the region of the head and neck,radiotherapy results in a series of complications in the patient, such as hypovascularization of the irradiated tissues, with reduced demand of oxygen and cells, and there is risk of the occurrence of osteoradionecrosis after tooth extraction, leading to the need for a differentiated approach to these patients. Of the therapeutic conduct adopted in the approach to irradiated patients, hyperbaric oxygen therapy is a well established resource, as the therapeutic principle of this modality enables the restoration of an adequate process of tissue repair. In this study, a clinical case is related, of the use of hyperbaric oxygen therapy as an adjuvant procedure, before and after multiple tooth extractions, in a patient irradiated in the head and neck region, obtaining success with cicatricial repair without complications.

  16. Balloon sinuplasty: a new concept in the endoscopic nasal surgery

    Nogueira Júnior, João Flávio


    Full Text Available Introduction: Sinus diseases affect millions of people annually. Clinical treatment is effective in most patients, but in case of failure of this therapy the functional endoscopic surgery is currently the treatment choice for surgical treatment. The objective of the functional endoscopic surgery is to increase the aeration and drainage of the involved paranasal sinuses, which allows for the adequate functioning of the nasal mucosa mucociliary clearance. However, this method still has some limitations, mainly because it removes the nasal mucosa and bone tissue, and it may lead to physiologic alterations of the nasosinusal mucosa and cicatricial fibrosis. Many of these patients could be benefited from less invasive methods, with larger nasal mucosa preservation. Since 2006, an even less invasive procedure was remarked in our specialty: the balloon dilatation of the paranasal sinus ostia. Objective: The objective of this article is to define the concept of sinuplasty, its action mechanism, and present the necessary material for the procedures performance; to describe the techniques with the equipment in a nasosinusal endoscopic surgery simulator model and review the current literature about the indications, complications, results, and follow-up of patients submitted to this procedure. Balloon sinuplasty is safe and appears to be effective in the improvement of the quality of life of patients not responsive to conventional clinical therapy. New applications and indications for this equipment should be described and researched.

  17. Lichen planopilaris: update on pathogenesis and treatment.

    Baibergenova, Akerke; Donovan, Jeff


    Lichen planopilaris (LPP) is considered to be a follicular variant of lichen planus. Clinical variants include classic LPP, frontal fibrosing alopecia, and the Graham-Little-Piccardi-Lassueur syndrome. The pathogenesis of LPP remains to be fully elucidated, but like other cicatricial alopecias involves the irreversible destruction of hair follicle stem cells and loss of a hair follicle's capacity to regenerate itself In the early stages of LPP, patients may have scalp pruritus, burning, tenderness, and increased hair shedding. A scalp biopsy shows a lymphocytic infiltrate involving the isthmus and infundibulum. Apoptotic cells present in the external root sheath and concentric fibrosis surrounds the hair follicle. Treatment is prescribed with the goal to alleviate patient symptoms and to halt the progression of hair loss. Treatment involves use of potent topical corticosteroids and/or intralesional corticosteroids. Options for systemic treatment include anti-inflammatory agents such as hydroxychloroquine, tetracyclines, pioglitazones, and immunosuppressive medications such as cyclosporine, mycophenolate mofetil, or systemic corticosteroids. Hair transplantation may also be an option if the disease has been in clinical remission. The management of LPP can sometimes be challenging and additional research is needed to improve outcomes for patients. PMID:23930355

  18. Delineating immune-mediated mechanisms underlying hair follicle destruction in the mouse mutant defolliculated.

    Ruge, Fiona; Glavini, Aikaterini; Gallimore, Awen M; Richards, Hannah E; Thomas, Christopher P; O'Donnell, Valerie B; Philpott, Michael P; Porter, Rebecca M


    Defolliculated (Gsdma3(Dfl)/+) mice have a hair loss phenotype that involves an aberrant hair cycle, altered sebaceous gland differentiation with reduced sebum production, chronic inflammation, and ultimately the loss of the hair follicle. Hair loss in these mice is similar to that seen in primary cicatricial, or scarring alopecias in which immune targeting of hair follicle stem cells has been proposed as a key factor resulting in permanent hair follicle destruction. In this study we examine the mechanism of hair loss in GsdmA3(Dfl)/+ mice. Aberrant expression patterns of stem cell markers during the hair cycle, in addition to aberrant behavior of the melanocytes leading to ectopic pigmentation of the hair follicle and epidermis, indicated the stem cell niche was not maintained. An autoimmune mechanism was excluded by crossing the mice with rag1-/- mice. However, large numbers of macrophages and increased expression of ICAM-1 were still present and may be involved either directly or indirectly in the hair loss. Reverse transcriptase-PCR (RT-PCR) and immunohistochemistry of sebaceous gland differentiation markers revealed reduced peroxisome proliferator-activated receptor-γ (PPARγ), a potential cause of reduced sebum production, as well as the potential involvement of the innate immune system in the hair loss. As reduced PPARγ expression has recently been implicated as a cause for lichen planopilaris, these mice may be useful for testing therapies. PMID:21160494

  19. Nasal meatus plasty: a contribution to plastic reconstruction of the nasal valve during midfacial degloving.

    Lorenz, Kai J; Maier, Heinz; Wilde, Frank


    Midfacial degloving is a proven method for easily accessing the midface, the nasal pyramid, the maxillary and ethmoidal sinuses, the orbits, as well as the anterior skull base. Indications for this method of access mainly include tumour resections in the area of the midface, the septum, the maxillary sinus, the paranasal to the sphenoidal sinus as well as the clivus. In addition, this method of access allows for the exposure of the bony structures of the midface in the event of extensive fractures. In general, this method of access combines an incision in the oral vestibule and circular incisions in the nasal vestibule area in order to release the nasal pyramid. After removing the facial wall of the maxillary sinus, extensive exposure of the surgical site is possible. One disadvantage of this method of access is the difficult reconstruction of the nasal valve area, which often leads to cicatricial stenoses and difficulties with breathing through the nose. Furthermore, wound healing problems and osteoradionecrosis in the area of the lateral margin of the anterior nasal aperture after replantation of the facial wall of the maxillary sinus have been described, because in this area sufficient soft tissue coverage cannot be ensured when a conventional technique is used. We describe a soft tissue flap pedicled in the cranial and caudal directions in the nasal valve area which makes both the reconstruction of the nasal vestibule and sufficient soft tissue coverage of the anterior nasal aperture possible. PMID:26504717

  20. Pulmonary langerhans cell histiocytosis

    Suri Harpreet S


    Full Text Available Abstract Pulmonary Langerhans Cell Histiocytosis (PLCH is a relatively uncommon lung disease that generally, but not invariably, occurs in cigarette smokers. The pathologic hallmark of PLCH is the accumulation of Langerhans and other inflammatory cells in small airways, resulting in the formation of nodular inflammatory lesions. While the overwhelming majority of patients are smokers, mechanisms by which smoking induces this disease are not known, but likely involve a combination of events resulting in enhanced recruitment and activation of Langerhans cells in small airways. Bronchiolar inflammation may be accompanied by variable lung interstitial and vascular involvement. While cellular inflammation is prominent in early disease, more advanced stages are characterized by cystic lung destruction, cicatricial scarring of airways, and pulmonary vascular remodeling. Pulmonary function is frequently abnormal at presentation. Imaging of the chest with high resolution chest CT scanning may show characteristic nodular and cystic abnormalities. Lung biopsy is necessary for a definitive diagnosis, although may not be required in instances were imaging findings are highly characteristic. There is no general consensus regarding the role of immunosuppressive therapy in smokers with PLCH. All smokers must be counseled on the importance of smoking cessation, which may result in regression of disease and obviate the need for systemic immunosuppressive therapy. The prognosis for most patients is relatively good, particularly if longitudinal lung function testing shows stability. Complications like pneumothoraces and secondary pulmonary hypertension may shorten life expectancy. Patients with progressive disease may require lung transplantation.

  1. [Magnetic resonance tomography in late sequelae of spinal and spinal cord injuries].

    Kravtsov, A K; Akhadov, T A; Sachkova, I Iu; Belov, S A; Chernenko, O A; Panova, M M


    Magnetic-resonance tomography (MRT) helped obtain a high-resolution image characterized by high sensitivity in respect of soft tissue contrast visualization and providing direct imaging of the spinal cord and its radicles. This method is useful in the diagnosis of injuries to the spine and cord. A total of 64 patients of both sexes aged 6 to 67 were examined. The primary diagnosis of traumatic changes in the spine and cord was confirmed by MRT in only 62% of cases. Two groups of patients were singled out: with acute and chronic injuries, subdivided into subgroups with and without spinal cord dysfunction. The detected changes were divided into extramedullary (traumatic disk hernias, compression of the cord or radicles with a dislocated bone fragment, epidural hematoma) and intramedullary (edema, hemorrhages, spinal cord disruption); MRT diagnosis of intramedullary changes is particularly important, more so in the absence of bone injuries. In remote periods after the trauma the clinical picture was determined by spinal canal stenosis, cicatricial atrophic and adhesive changes eventually blocking the liquor space. Intramedullary changes presented as spinal cord cysts or syringomyelia. A classification of the detected changes by the types of injuries and their aftereffects is presented in the paper. The authors emphasize the desirability of MRT in spinal injuries with signs of cord dysfunction. PMID:7801568

  2. Mucous membrane pemphigoid with exclusive gingival involvement: Report of a case and review of literature

    Shamimul Hasan


    Full Text Available According to Sir William Osler, Mouth is the mirror of the body which reflects systemic -diseases. The oral mucosa may be affected by a variety of mucocutaneous diseases and oral lesions may occur first or very early in several mucocutaneous disorders. The erosive gingival lesions associated with vesiculobullous diseases such as lichen planus, cicatricial pemphigoid, and pemphigus vulgaris have been collectively referred to as "Desquamative gingivitis" (DG. Gingival desquamation is a clinical sign in which the gingiva appears reddish, painful, glazed and friable with destruction of the epithelium. This gingival desquamation is due to various disease processes in gingiva. The disease process may be a localized disease of gingiva or a systemic disease which manifests in the gingiva. It is important to be aware of this rare clinical entity so as to distinguish DG from plaque induced gingivitis which is an extremely common condition, easily recognized and treated daily by the dental surgeon. Accurate diagnosis and effective treatment of these lesions may greatly diminish or reverse disease progression. Here by, we present a case of mucous membrane pemphigoid presenting as gingival desquamation in a 45 year old female. Our patient presented with generalized erythematous gingiva and gingival desquamation involving the free, attached and marginal gingiva of left maxillary and mandibular dentition. However, other mucosal and skin involvement was not appreciated in the present case. Thorough history, clinical examination, histopathology and immunofluorescence studies helped us to arrive at the diagnosis of this rare sub-epithelial blistering disorder.

  3. Medical and surgical therapies for alopecias in black women.

    Callender, Valerie D; McMichael, Amy J; Cohen, George F


    Hair loss is a common problem that challenges the patient and clinician with a host of cosmetic, psychological and medical issues. Alopecia occurs in both men and women, and in all racial and ethnic populations, but the etiology varies considerably from group to group. In black women, many forms of alopecia are associated with hair-care practices (e.g., traction alopecia, trichorrhexis nodosa, and central centrifugal cicatricial alopecia). The use of thermal or chemical hair straightening, and hair braiding or weaving are examples of styling techniques that place African American women at high risk for various "traumatic" alopecias. Although the exact cause of these alopecias is unknown, a multifactorial etiology including both genetic and environmental factors is suspected. A careful history and physical examination, together with an acute sensitivity to the patient's perceptions (e.g., self-esteem and social problems), are critical in determining the best therapy course. Therapeutic options for these patients range from alteration of current hair grooming practices or products, to use of specific medical treatments, to hair replacement surgery. Since early intervention is often a key to preventing irreversible alopecia, the purpose of the present article is to educate the dermatologist on all aspects of therapy for hair loss in black women--including not only a discussion of the main medical and surgical therapies but also an overview of ethnic hair cosmetics, specific suggestions for alterations of hair-care practices, and recommendations for patient education and compliance. PMID:15113284

  4. Robot-assisted surgery of the shoulder girdle and brachial plexus.

    Facca, Sybille; Hendriks, Sarah; Mantovani, Gustavo; Selber, Jesse C; Liverneaux, Philippe


    New developments in the surgery of the brachial plexus include the use of less invasive surgical approaches and more precise techniques. The theoretical advantages of the use of robotics versus endoscopy are the disappearance of physiological tremor, three-dimensional vision, high definition, magnification, and superior ergonomics. On a fresh cadaver, a dissection space was created and maintained by insufflation of CO2. The supraclavicular brachial plexus was dissected using the da Vinci robot (Intuitive Surgical, Sunnyvale, CA). A segment of the C5 nerve root was grafted robotically. A series of eight clinical cases of nerve damage around the shoulder girdle were operated on using the da Vinci robot. The ability to perform successful microneural repair was confirmed in both the authors' clinical and experimental studies, but the entire potential of robotically assisted microneural surgery was not realized during these initial cases because an open incision was still required. Robotic-assisted surgery of the shoulder girdle and brachial plexus is still in its early stages. It would be ideal to have even finer and more suitable instruments to apply fibrin glue or electrostimulation in nerve surgery. Nevertheless, the prospects of minimally invasive techniques would allow acute and subacute surgical approach of traumatic brachial plexus palsy safely, without significant and cicatricial morbidity. PMID:24872778

  5. Clinicoepidemiological Observational Study of Acquired Alopecias in Females Correlating with Anemia and Thyroid Function.

    Deo, Kirti; Sharma, Yugal K; Wadhokar, Meenakshi; Tyagi, Neha


    Alopecia can either be inherited or acquired; the latter, more common, can be diffuse, patterned, and focal, each having cicatricial and noncicatricial forms. This observational study of 135 cases in a semiurban Indian population aimed to detect the prevalence of various forms of acquired alopecia in females and correlate the same with levels of hemoglobin, serum ferritin, triiodothyronine, thyroxin, and thyroid stimulating hormone. The majority (84, 62.2%) of our cases of alopecia had telogen effluvium followed by female pattern alopecia (32, 23.7%). Stress (86, 63.7%), topical application of chemicals (72, 53.3%), systemic medications for concurrent illnesses (62, 5%), and pregnancy (14, 10.3%) were the common exacerbating factors. Neither low hemoglobin (12 μg/L. Though lack of vitamin B12 testing was a limitation of our study, its deficiency could be the probable cause of iron deficiency as the majority (58, 64.4%) of these cases, as indeed majority (89, 65.4%) of our study population, were vegetarians. Thyroid disorders (23, 17%, including 9 newly diagnosed) were not of significance statistically. PMID:26904106

  6. [Tendinopathy of the patellar ligament secondary to transtendineal arthroscopy of the knee. Ultrasonographic evaluation].

    Martino, F; Ettorre, G C; Macarini, L; Tritto, D; Patella, V; Fracchiolla, M; Moretti, B; Cafaro, F


    During knee arthroscopy, the transtendinous approach best visualizes articular structures, even though it may cause patellar tendinitis, following the surgical procedures. Thirty patients (22 men and 8 women) who had undergone transtendinous arthroscopy of the knee were submitted to clinical and US follow-up at 3, 6, 12 months. This monitoring was aimed at correlating clinical and instrumental findings of the above iatrogenic condition. Painful symptoms were present in 30% of the cases in group I, in 10% of group II and in no patient in group III. US demonstrated thickening of the patellar tendon in all the three groups of patients, with reduced echogenicity and blurred margins at the arthroscopic portal, plus decreasing gravity over time. The US pattern of arthroscopic surgical gap was observed in all patients in group I, in 10% of patients in group II and in none of the patients in group III. In conclusion because of its clinical course and of its US findings, in our experience patellar tendinopathy following transtendinous arthroscopy of the knee is not to be considered as a iatrogenic inflammatory or degenerative sequela, but as a physiological cicatricial evolution of the surgical transtendinous breach. The US follow-up of the latter allows the correct assessment of the recovering process. PMID:8272543

  7. Physical and mathematical aspects of blood-glucose- and insulin-level kinetics in patients with coronary heart disease and high risk of its development

    Denisova, Tatyana P.; Malinova, Lidia I.; Malinov, Igor A.


    The intravenous glucose tolerance test was performed to estimate the kinetics of blood glucose and insulin levels. Glucose was injected in individual standardized dose (0.5 g. per 1 kg of body weight). Three groups of patients were checked up: 1) patients with coronary heart disease verified by cicatricial alterations in myocardium found by electrocardiographic and echocardiographic methods; 2) children of patients with transmural myocardial infarction practically healthy at the moment of study; 3) persons practically healthy at the moment of study without any indications on cardiovascular diseases and non-insulin dependent diabetes mellitus among all ancestors and relatives who frequently were long-livers. Last groups didn't differ by age and sex. Peripheral blood glucose level, immunoreactive and free insulin (tested by muscular tissue) were studied just before glucose injection (on an empty stomach) and 4 times after it. The received discrete data were approximated by high degree polynomials, the estimation of blood glucose and insulin time functions symmetric was performed. The deceleration of degradation of insulin circulating in peripheral blood and the time decrease of second phase of insulin secretion were analytically established. This fact proves the complicated mechanism of insulin alterations in atherosclerosis, consisting not only of insulin resistance of peripheral tissues but of decrease of plastic processes in insulin- generating cells.

  8. The influence of low-level laser therapy on biomodulation of collagen and elastic fibers A influência da terapia a laser de baixa densidade de energia na biomodulação das fibras colágenas e elásticas

    Lívia Souza Pugliese


    Full Text Available The study of low-level laser therapy upon extracellular matrix elements is important to understand the wound healing process under this agent. However, little is known about the interference of laser light in relation to collagen and elastic fibers. Cutaneous wounds were performed on the back of 72 Wistar rats and a Ga-Al-As low-level laser was punctually applied with different energy densities. The animals were killed after 24, 48, 72 hours and 5, 7 and 14 days. Tissues were stained with hematoxilin-eosin, sirius red fast green and orcein and then analyzed. It was observed that the treated group exhibited larger reduction of edema and inflammatory infiltrate. The treated animals presented a larger expression of collagen and elastic fibers, although without statistical significance (p > 0.05. Treatment with a dosage of 4 J/cm² exhibited more expressive results than that with 8 J/cm². In this study, the authors concluded that low-level laser therapy contributed to a larger expression of collagen and elastic fibers during the early phases of the wound healing process.O estudo da terapia a laser de baixa densidade de energia sobre os diversos constituintes da matriz extracelular é crucial para o entendimento do processo cicatricial sob esse agente. Todavia, pouco se sabe sobre a interferência do laser em relação às fibras colágenas e elásticas. Realizaram-se ferimentos cutâneos padronizados no dorso de setenta e dois ratos Wistar e, em seguida, aplicação pontual do raio laser de baixa potência do tipo Arseneto de Gálio-Alumínio (Ga-Al-As com diferentes densidades de energia. Os animais foram sacrificados com 24, 48 e 72 horas e aos 5, 7 e 14 dias. Procedeu-se à análise das secções teciduais coradas por hematoxilina-eosina, sírius vermelho e orceína. Observou-se, que nos grupos submetidos à terapia a laser, houve maior redução do edema e infiltrado inflamatório. Os animais tratados apresentaram uma maior expressão de fibras


    Weslay Souza de Oliveira


    Full Text Available The aim of this study was to evaluate the effect of transcutaneous electrical stimulation with alternate electrical current on gastrocnemius muscle tendon healing, in rabbits submitted to experimental tenotomy and tenorrhaphy. Twenty-four male rabbits, New Zealand breed, were divided into four groups of the same number, in order to evaluate the cicatricial tendon repair at 7, 15, 21 and 30 days post-surgery. The middle third of the tendon of the gastocnemius muscle was sectioned transversall and submitted to a synthesis with Kessler modified suture. After 24 hours, daily at the same schedule, were done near the cutaneous wound of the right pelvic member, applications of 2 mA of alternative current, during six minutes, at a frequence of 100 Hz. The material collected was examined by light microscopy. Significant difference wasn´t found between the treated member and the control. Transcutaneous electrotherapy was used in our experimental protocol, despite not leading to tissue damage and being less invasive in comparison to other methods, was incapable to promote a more efficient healing response on the gastrocnemius muscle tendon repair in rabbits.

    KEY WORDS: Achilles tendon, electrotherapy, surgery, tenotomy, tissue repair. Este trabalho teve como objetivo avaliar o efeito da estimulação elétrica transcutânea de corrente alternada na regeneração do tendão do músculo gastrocnêmio, em coelhos submetidos à tenotomia e tenorrafia experimental. Dividiram-se vinte e quatro coelhos adultos da raça Nova Zelândia em quatro grupos iguais para avaliação da cicatrização tendínea aos sete, quinze, vinte e um e trinta dias de pós-operatório. Após incisão cutânea de aproximadamente três centímetros, o terço médio do tendão do músculo gastrocnêmio foi individualizado, seccionado transversalmente e suturado com sutura de Kessler modificada. Decorridas 24 horas, diariamente em um mesmo horário aplicaram-se, próxima


    Celso Fernando Ribeiro Araújo


    Full Text Available Desde os mais remotos tempos pesquisam-se incessantemente drogas que acelerem o processo cicatricial normal. No Hospital de Clínicas da UFPr preconiza-se a limpeza de feridas limpas com solução salina de NaCl a 0,9%. Alguns serviços têm utilizado empiricamente o Agarol® e o Trigliceril® na confecção do curativo após este procedimento. Este estudo objetivou avaliar experimentalmente a evolução de feridas cutâneas, em ratos, produzidas por incisão cirúrgica tratadas com solução de NaCl a 0,9%, Agarol® e Trigliceril® . Utilizou-se 24 ratos, divididos em três grupos: C, controle, submetido à troca diária de curativo após limpeza com solução de NaCl a 0,9%; grupo A com aplicação de Agarol® e grupo T com aplicação de Trigliceril®. Verificou-se que no terceiro e décimo quarto dias de pós-operatório o Agarolâ e o Triglicerilâ não influenciaram o processo cicatricial. No sétimo dia de pós-operatório verificou-se houve aumento da quantidade de tecido de granulação nos grupos tratados com Agarolâ e diminuição da neovascularização no grupo Triglicerilâ .For centuries, mankind has been incessantly researching with drugs that could accelerate the normal wound healing process. In the Hospital de Clinicas da Universidade Federal do Paraná, it has been recommended that the cleansing of clean wounds with saline solution of 0.9% sodium chloride, followed by a cover of dry dressing. However, some services have empirically used Agarol and Trigliceril in the confection of dressings after cleansing. This study aimed to evaluate the evolution of cutaneous wounds treated with normal saline, Agarol and Trigliceril in rats. A group of twenty-four rats were submitted to three skin circular incisions of 1.2 cm in diameter on their backs which exposed the subcutaneous tissue. The wounds were cleaned daily with normal saline solution. This group was distributed at random within three groups: Group C, control, received a clean

  11. Avaliação da deposição de colágeno após implante de fáscia lata e de gordura na prega vocal de coelho: estudo histomorfométrico Assessment of collagen deposits after implant of fascia lata and fat in the vocal folds of rabbits: histomorphometric study

    Christiano de Giacomo Carneiro


    Full Text Available Vários materiais têm sido injetados ou inseridos em pregas vocais na tentativa de solucionar a incompetência glótica. Contudo, poucos são os estudos que avaliam o processo cicatricial decorrente da enxertia destes materiais. OBJETIVO: O objetivo desta pesquisa foi avaliar a concentração de colágeno após enxerto microcirúrgico de fáscia muscular e gordura na prega vocal de coelho FORMA DE ESTUDO: experimental MATERIAL E MÉTODO: Dezenove coelhos foram submetidos à inserção de enxerto na prega vocal direita, sendo nove de fáscia e dez de gordura. A prega vocal esquerda foi submetida ao mesmo procedimento, sem enxertia. Os coelhos foram sacrificados após 90 e 180 dias. O colágeno foi analisado morfometricamente através do método da Picrossírius-polarização com a utilização do software Image Pro Plus RESULTADOS: Houve aumento do colágeno em todos grupos enxertados quando comparados com o grupo controle. A concentração do colágeno encontrada nos coelhos submetidos a enxerto de gordura foi significativamente maior quando comparados à concentração do colágeno nos coelhos submetidos a enxerto de fáscia muscular, tanto com 90 quanto com 180 dias CONCLUSÃO: A enxertia de gordura e fáscia lata na prega vocal de coelho promoveu maior deposição de colágeno do que no grupo controle, sendo mais exuberante na inserção de gordura.Several materials have been injected or introduced in the vocal folds in attempt of solving the glottic insufficiency. However, few studies have evaluated the cicatricial process due to the implantation of these materials. AIM: The objective of this research was to evaluate the concentration of collagen after microsurgery graft of muscular fascia and fat in the vocal folds of rabbit STUDY DESIDN: experimental MATERIAL AND METHOD: Nineteen rabbits were submitted to the graft insert in the right vocal fold, being nine of fascia and ten of fat. The left vocal fold was submitted to the same process

  12. Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation Efeitos da aplicação tópica da ciclosporina A a 2% sobre a córnea de cães submetidos à ceratoplastia lamelar com implante de pericárdio de eqüino preservado em glicerina: avaliação clínica e morfológica

    Roseli Borges Teixeira


    Full Text Available The effects of topical 2% cyclosporine A on the cornea of dogs subjected to experimental lamellar keratoplasty with an equine pericardial graft were evaluated. Ten dogs were grouped to be evaluated 3, 7, 15, 30 and 60 days after surgery. Animals received bilateral grafts followed by the application of a 2% cyclosporine A ointment on the left eye (treated eye and the ointment base on the right eye (control eye twice a day. The ophthalmic evaluation showed profound bilateral blepharospasm, photophobia and a mucous secretion until the 7th day after surgery; corneal vascularization starting in the limbus was observed as early as the 3rd day in both eyes; opacification and vascularization were more intense in the treated cornea on days 15 and 30 after surgery. Vascularization was still evident on the 60th day, and looked similar in treated eyes and control eyes. The histologic evaluation showed a complete bilateral reepithelization and corneal vascularization three days after the surgery; intense vascularization in both eyes on days 15 and 30, that was much more pronounced in the treated cornea; and a bilateral predominance of polymorphonuclear cells until day 15, and mononuclear cells on day 30. Intact epithelium and stroma with new vessels, as well as graft absorption, in both eyes, were seen on the 60th day. The lamellar keratoplasty with equine pericardial graft was an effective model to study the inflammatory kinetics and corneal vascularization phenomenon. In this study, cyclosporine A did not inhibit corneal vascularization and it did not interfere in the corneal cicatricial process.Foram avaliados os efeitos da ciclosporina A a 2% sobre a córnea de cães submetidos à ceratoplastia lamelar experimental com implante de pericárdio de equino. Dez cães foram divididos em grupos para estudo aos três, sete, 15, 30 e 60 dias de pós-operatório, recebendo implantes bilaterais e em seguida aplicação da pomada com ciclosporina A a 2% no olho

  13. Efeitos da terapia física descongestiva na cicatrização de úlceras venosas Efectos de la terapia física descongestiva en la cicatrización de úlceras venosas Effects of the decongestive physiotherapy in the healing of venous ulcers

    Roberta Azoubel


    Full Text Available Objetivou-se neste estudo verificar os efeitos da terapia física descongestiva (TFD na cicatrização de úlceras venosas. Trata-se de um estudo intervencionista, quase experimental, do qual participaram 20 clientes, divididos em 2 grupos: o grupo controle (n=10 e o grupo de intervenção (n=10. Os clientes do primeiro grupo foram tratados apenas com curativo convencional e os do segundo grupo, com curativo convencional e terapia física descongestiva (associação de técnicas: drenagem linfática manual, enfaixamento compressivo, elevação dos membros inferiores, exercícios miolinfocinéticos e cuidados com a pele. Ambos os grupos foram tratados durante seis meses. Os clientes submetidos à TFD apresentaram significante redução de edema e da dor, além de melhora no processo cicatricial. Os resultados permitiram verificar que a terapia descongestiva estimula o processo de cicatrização de úlceras venosas, melhorando a qualidade de vida dos indivíduos.En este estudio se objetivó verificar los efectos de la terapia física descongestiva (TFD en la cicatrización de úlceras venosas. Se trató de un estudio intervencionista, casi experimental, del cual participaron veinte pacientes que constituyeron dos grupos: el grupo control (n=10 y el grupo de intervención (n=10. Los pacientes del primer grupo fueron tratados apenas con curaciones convencionales, mientras que los del segundo grupo recibieron curación convencional y terapia física descongestiva (asociación de técnicas: drenaje linfático manual, fajamiento compresivo, elevación de los miembros inferiores, ejercicios miolinfocinéticos y cuidados con la piel. Ambos grupos fueron tratados durante seis meses. Los pacientes sometidos a TFD presentaron significativa reducción de edema y dolor, y mejora en el proceso cicatricial. Los resultados permitieron verificar que la terapia descongestiva estimula el proceso de cicatrización de úlceras venosas, mejorando la calidad de vida de

  14. Histopathology of human American cutaneous leishmaniasis before and after treatment Histopatologia de leishmaniose tegumentar americana humana, antes e após o tratamento

    A.C.C. Botelho


    Full Text Available Chemical therapy for the treatment of leishmaniasis is still inadequate, and a number of drugs and therapeutic programs are being tested. Besides treatment, the ultimate goal is an effective cure, and histopathological analyses of the lesion cicatrices constitute an important measure of treatment success, or otherwise, in this respect. In this paper, we describe histopathological patterns in cases of American cutaneous leishmaniasis in 32 patients from the municipality of Caratinga, Minas Gerais, Brazil, before and after treatment with the following therapeutic methodos: l leishvacin + glucantime; 2 leishvacin + BCG associated with glucantime; 3 glucantime; 4 leishvacin + BCG. Lesion fragments were collected from all patients by biopsy prior to, and approximately 30 days after, each treatment which resulted in a clinical diagnosis of cure. Following the analysis of slides, the preparations were described from a histopathological point of view and grouped taking into account the prevalence or significance of the characteristic elements. This process resulted in the following classification: 1. exsudative reaction (ER; 2. exsudative giant cell reaction (EGCR; 3. exsudative productive reaction (EPR; 4. exsudative productive giant cell reaction (EPGCR; 5. exsudative productive necrotic reaction (EPNR; 6. necrotic exsudative reaction (NER; 7. productive exsudative reaction (PER, 8. productive giant cell reaction (PGCR; 9. productive exsudative giant cell reaction (PEGCR; 10. productive exsudative giant cell granulomatous reaction (PEGCGR; 11. productive reaction (PR and 12. productive cicatricial (cure reaction (PCR. After this analysis, it was noted that clinical cure did not always coincide with histopathological cure.A quimioterapia para a leishmaniose não é satisfatória e existem hoje, várias drogas e esquemas terapêuticos em teste. Além do tratamento ideal, busca-se um critério de cura efetivo, onde a análise da histopatologia da

  15. Regeneração hepática induzida por ressecção segmentar do fígado, em rato Hepatic regeneration induced by segmental liver resection, in rats

    Andy Petroianu


    Full Text Available OBJETIVO: Avaliar a regeneração progressiva do parênquima hepatocitário, nos seus aspectos macro e microscópicos, em pós-operatório imediato e tardio de ressecção segmentar do fígado. MÉTODO: Foram estudados 10 ratos machos albinos da raça Wistar, pesando entre 250 e 300 gramas, submetidos à hepatectomia parcial de lobo esquerdo, com retirada de cerca de 20% da massa total do órgão. Os animais foram divididos aleatoriamente em dois grupos (n=5 para estudo no 7º (Grupo 1 e 21º (Grupo 2 dias pós-operatórios. Decorrido o tempo de acompanhamento, avaliou-se o aspecto macroscópico e microscópico do fígado. RESULTADOS: No Grupo 1, após sete dias, os animais apresentavam a cavidade abdominal com poucas aderências. O fígado mostrava reação cicatricial no local da ressecção, porém seu tamanho já era próximo ao normal. O exame histopatológico mostrou freqüentes sinais de poliploidia dos hepatócitos, além de tecido de granulação frouxo e desordenado, acompanhado de escasso infiltrado de células inflamatórias. O Grupo 2, após 21 dias, mostrava poucas aderências na cavidade abdominal, e o fígado com aspecto e dimensões próximos ao normal. A histologia mostrou tecido cicatricial mais denso, ordenado, sem sinais inflamatórios. Observou-se apenas pequeno grau de poliploidia hepatocitária. CONCLUSÃO: Após remoção cirúrgica de 20% do parênquima hepático houve aumento temporário da renovação celular verificado por poliploidia hepatocitária.BACKGROUND: To assess the progression of liver regeneration after partial hepatic resection. METHODS: Ten Wistar adult rats, of both sexes were studied. After anesthesia with ether, the animals were submitted to a left lobe parcial hepatectomy . Rats were randomly divided into two groups (n=5 according to the day macro and microscopic studies were carried out, 7th (Group 1 or 21st postoperative days. RESULTS: All rats survived throughout the experimental protocol. The

  16. Reparación quirúrgica de fístula traqueocutánea y de cicatriz postraqueotomía mediante colgajo dermograso de rotación Reparação cirúrgica de fístula traqueocutânea e de cicatriz pós-traqueostomia com rotação de retalho dermoadiposo Surgical repair of tracheocutaneous fistulae and postracheotomy scar using a dermoadipose turn-over flap

    F. Passos da Rocha


    Full Text Available La fístula traqueocutánea y la retracción cicatricial son las complicaciones postraqueotomía más frecuentes. El objetivo del presente trabajo es el presentar una técnica quirúrgica de fácil ejecución para la reparación de los defectos estéticos y funcionales creados por estas patologías. Presentamos el caso de un varón de 61 años que había sufrido traqueotomía. Tras la decanulación, la cicatrización cursó con retracción, adherencia a planos profundos y aparición de fístula traqueocutánea. El cierre de la fístula se hizo reparando el defecto funcional mediante la técnica de rotación de colgajos dermograsos en "hoja de libro", logrando el cierre del área de retracción y reparando el defecto estético. La retracción cutáneo-hipodérmica es un defecto inestético, incómodo y estigmatizante. Las fístulas traqueocutáneas con escape de aire son desagradables tanto desde el punto de vista funcional como estético. El método que presentamos logró corregir la fístula y la depresión provocada por la traqueotomía mediante un procedimiento simple, rápido y efectivo. Este método evita las dificultades encontradas en otras técnicas y garantiza la corrección funcional y estética empleando un método simple con baja morbilidad.Tracheocutaneous fistulas and scar retraction are frequent complications of the tracheotomy. The objective of the present manuscript is to present an easy execution surgical technique to restore tracheocutaneous fistula and cutaneous retraction. We present the clinical case of a 61 years old man who had suffered tracheotomy. After decannulation, the scar became retracted and adhered to deep tissues, developing into a tracheocutaneous fistula. The fistula was closed repairing the functional defect and a turn-over hinge flap of dermoadipose tissue was used to fill the retracted area, correcting the aesthetic abnormality. Correction of cicatricial retraction and of tracheocutaneous fistula using the

  17. Aspectos clínicos e macroscópicos da palatoplastia imediata com implante de cartilagem da pina articular, conservada em glicerina a 98%, após indução experimental de fenda palatina em cães Clinic and macroscopic view of immediate palatoplasty with auricular pinna cartilage preserved in 98% glycerin in canine experimental palat cleft

    Emerson Antônio Contesini


    Full Text Available Os defeitos de espessura completa do palato que resultam em comunicação oro-nasal raramente cicatrizam espontaneamente. Eles requerem reparo cirúrgico, porém, pode ser difícil obter cicatrização satisfatória devido às condições próprias das cavidades nasal e oral. Neste experimento, foram utilizados 14 cães, reunidos em dois grupos de igual número. Todos animais foram submetidos à indução de fenda palatina experimental. O grupo G1 foi o controle, cuja palatoplastia foi realizada a partir da confecção de "flap" muco-periósteo. No grupo G2, foi utilizada cartilagem da pina auricular conservada em glicerina a 98% entre o osso palatino e o muco-periósteo. Os animais foram avaliados macroscopicamente quanto ao processo cicatricial, por radiografias para a regeneração óssea e por rinoscopia para observar a regeneração da mucosa nasal. Foi observada a cicatrização da mucosa oral a partir do 10º dia, com deiscência de sutura em dois animais do grupo G1. A radiografia demonstrou evolução na cicatrização óssea, num processo mais acelerado no grupo G2 quando comparado ao grupo G1. A rinoscopia revelou, aos 60 dias, completa regeneração da mucosa nasal em ambos os grupos. Ao final do período de avaliação foi observada macroscopicamente, completa fusão óssea nos animais do grupo G2 e incompleta nos do grupo G1, o que demonstrou a interferência benéfica do implante no processo cicatricial do grupo G2.The incomplete defects of hard palate result in oro-nasal fistula seldom cure themselves in a spontaneous way, due to limited condition in oral and nasal cavity it may be difficult to obtain satisfactory cicatrization, requiring surgery to interfere in the healing process. Fourteen dogs arranged in two groups were submitted to experimental hard palate cleft. The G1 group was the control group, in which palatoplasty was achieved with mucous-periosteum flap. In the G2 group, a preserved at 98% glycerin auricular pinna

  18. Cicatrização de feridas Wound healing

    Antonio Carlos Ligocki Campos


    Full Text Available INTRODUÇÃO: A cicatrização de feridas consiste em perfeita e coordenada cascata de eventos que culminam com a reconstituição tecidual. O processo cicatricial é comum a todas as feridas, independe do agente que a causou. O processo de cicatrização é dividido didaticamente em três fases: inflamatória, proliferação ou granulação e remodelamento ou maturação. O colágeno é a proteína mais abundante no corpo humano e também é o principal componente da matriz extracelular dos tecidos. Estrutura-se numa rede densa e dinâmica resultante da sua constante deposição e reabsorção. O tecido cicatricial é resultado da interação entre sua síntese, fixação e degradação. Existem várias maneiras de avaliar a cicatrização das feridas. Os métodos mais utilizados atualmente são a tensiometria, a densitometria e morfometria do colágeno, a imunoistoquímica e, mais recentemente, a dosagem de fatores de crescimento. MÉTODOS: Foram revisadas todas as dissertações de mestrado e teses de doutorado realizadas na linha de pesquisa "Cicatrização de órgãos e tecidos" do programa de Pós-Graduação em Clínica Cirúrgica da Universidade Federal do Paraná. Foi realizada, também, revisão atualizada da literatura internacional no Pubmed e nacional. CONCLUSÃO: A cicatrização é um processo complexo, que começou a ser entendido em maior amplitude nos últimos anos. Recentes pesquisas têm sido direcionadas para atuar na modulação da cicatrização a nível molecular, na tentativa de evitar completamente cicatrizes patológicas.BACKGROUND: The wound healing consists of a perfect and coordinated cascade of events that result in tissue reconstitution. The healing process is common to all wounds, independently of the agent that has caused it. It is divided didactically into three phases: inflammation, proliferation or granulation and remodelation or maturation. The collagen is the most abundant protein in the human body and is

  19. Membrana de PLDLA-TMC como protetor na regeneração do tendão calcâneo: Estudo in vivo em coelhos

    Julia Rezende Duek


    Full Text Available Um dos maiores problemas da cirurgia restauradora dos tendões flexores é evitar a formação de aderências, pois limita seu deslizamento. Assim, é muito desejável que a cicatrização ocorra o mais rápido possível, restaurando a capacidade de deslizamento dos tendões, sem limitações dos movimentos. O objetivo deste trabalho foi analisar a resistência do Tendão calcâneo, utilizando membrana de PLDLA-TMC como protetor da aderência dos tecidos adjacentes após implantes em coelhos New Zeland. Foram utilizados 20 coelhos, divididos em dois grupos, os quais foram submetidos à secção do tendão calcâneo e posterior sutura com técnica de Kessler modificada. No grupo A, logo após sutura o animal foi sacrificado e o tendão foi enviado para ensaio mecânico. Já no grupo B, após três semanas, os animais foram sacrificados e o material foi submetido à análise histológica. Os resultados obtidos a partir do ensaio mecânico mostraram melhora nos valores da resistência à tração dos implantes com a membrana, comparados aos apenas suturados. Na cicatrização da lesão, ocorreu melhor organização das fibras colágenas quando foi utilizado o polímero PLDLA-TMC, além de uma menor aderência cicatricial. Conclui-se que a membrana de PLDLA-TMC é promissora na reparação de lesões tendíneas.

  20. Uso de concentrados autólogos de plaquetas como terapia regenerativa de enfermedades crónicas del aparato musculoesquelético equino Use of autologous platelet concentrates as regenerative therapy for chronic diseases of the equine musculoskeletal system

    JU Carmona


    Full Text Available Las plaquetas son fundamentales para la reparación tisular de las heridas, ya que secretan factores de crecimiento, los cuales inducen quimiotaxis, proliferación y diferenciación celular, neovascularización y producción de la matriz extracelular. Se ha propuesto el uso de concentrados autólogos de plaquetas (APCs para acelerar la cicatrización de heridas, disminuir la inflamación, estimular la capacidad regenerativa de los tejidos lesionados, disminuir la actividad fibroblástica y la producción de tejido cicatricial no-funcional. Los APCs pueden ser preparados mediante diferentes métodos. Cada método produce APCs de diferente calidad celular y molecular. Recientemente, se ha generado información básica y clínica que justifica el uso de APCs en caballos con enfermedades degenerativas del aparato musculoesquelético equino como la osteoartritis, tendinopatías y desmopatías.Platelets are of pivotal importance for wound healing since they release growth factors that in turns produce chemotaxis, both cellular proliferation and differentiation, angiogenesis, and extracellular matrix deposition. The use of autologous platelet concentrates (APCs has been proposed for accelerating wound healing, decreasing inflammation, to stimulate the regenerative capability of the injured tissues, to decrease the fibroblastic activity and to avoid the production of non functional scarring tissue. APCs could be obtained by several methods. Each method produces APCs of different both cellular and molecular quality. Recently, some basic and clinical information that justifies the usage of APCs for the treatment of degenerative musculoskeletal diseases in horses, such as osteoarthritis, tendinopathies and desmopathies has been published.

  1. Collagen IV in normal skin and in pathological processes

    Ana Maria Abreu-Velez


    Full Text Available Context: Type IV collagen is a type of collagen found primarily in the skin within the basement membrane zone. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers are thus link head-to-head, rather than in a parallel fashion. Also, type IV collagen lacks a glycine in every third amino-acid residue necessary for the tight collagen helix. Thus, the overall collagen-IV conformation is structurally more pliable and kinked, relative to other collagen subtypes. These structural features allow collagen IV to form sheets, which is the primary structural form found in the cutaneous basal lamina. There are six human genes associated with collagen IV, specifically COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6. The aim of this review is to highlight the significance of this protein in normal skin, and in selected diseases. Results: The alpha 3 protein constituent of type IV collagen is thought to be the antigen implicated in Goodpasture′s syndrome, wherein the immune system attacks the basement membranes of the renal glomeruli and pulmonary alveoli. In addition, mutations to the genes coding for type IV collagen lead to the Alport syndrome. Furthermore, autoantibodies directed against denatured human type IV collagen have been described in rheumatoid arthritis, scleroderma, and SLE. Structural studies of collagen IV have been utilized to differentiate between subepidermal blistering diseases, including bullous pemphigoid, acquired epidermolysis bullosa, anti-epiligrin cicatricial pemphigoid, and bullous lupus erythematosus. Collagen IV is also of importance in wound healing and in embryogenesis. Conclusions: Pathological studies have demonstrated that minor structural differences in collagen IV can lead to distinct, clinically different diseases.

  2. Experiencia con el colgajo de Kirschbaum en secuelas de quemaduras cérvico-faciales

    P. Rossell-Perry


    Full Text Available Presentamos nuestra experiencia con el colgajo de Kirschbaum (colgajo en charretera en la corrección de retracciones cicatriciales de la región cervical anterior y del tercio inferior de la cara. Llevamos a cabo un estudio retrospectivo sobre una serie de 63 pacientes con retracciones cicatriciales de la región cervical anterior y del tercio inferior facial, operados por el primer autor entre los años 1996 y 2012 dentro del marco de las campañas quirúrgicas desarrolladas a lo largo del país (Perú en las áreas geográficas con mayor necesidad de atención especializada. En todos los casos empleamos el colgajo en charretera tomado de la región lateral del cuello y de la región deltoidea. Evaluamos a los pacientes mediante el examen físico y la documentación fotográfica obtenida en los periodos pre y postoperatorio. Obtuvimos mejoría en diferentes grados de la limitación funcional de la región cervical y facial en todos los casos operados, con viabilidad total del colgajo en 59 casos (93,65 % y parcial en 4 casos (6,34 % . No hubo ningún caso de pérdida total. Las complicaciones recogidas fueron pocas, principalmente hematomas, 6 casos (9,52 % e infección, 2 casos (3,17 % . Las zonas donantes evolucionaron con cicatriz hipertrófica en 22 de los casos (34,92 % . En conclusión, nuestra experiencia con el uso de esta técnica quirúrgica ha demostrado ser de utilidad en el tratamiento de las secuelas de quemadura con retracción cicatricial cervical y facial, por lo que consideramos que el colgajo de Kirschbaum es seguro y tiene pocas complicaciones.

  3. Sarcoidose cutânea sobre cicatrizes: relato de caso Sarcoidosis on skin scars: a case report

    Sônia Antunes de Oliveira Mantese


    Full Text Available A sarcoidose é uma doença inflamatória sistêmica, de etiologia desconhecida, em que granulomas não caseosos são encontrados nos órgãos acometidos. O envolvimento cutâneo ocorre em 25% dos casos, com grande polimorfismo lesional. O acometimento de cicatrizes é incomum, porém clinicamente característico de sarcoidose cutânea. A maioria dos pacientes com sarcoidose cicatricial tem doença sistêmica. Relata-se o caso de uma paciente de 65 anos, que apresentou nodulações sobre cicatrizes 20 anos, após a realização de procedimentos cirúrgicos, sem manifestações sistêmicas. Salienta-se a importância de se investigar sarcoidose em cicatrizes prévias, com alterações inflamatórias.Sarcoidosis is a systemic inflammatory disorder of unknown origin, in which non-caseating granulomas (small inflammatory nodules are found in the affected organs. Cutaneous involvement occurs in 25% of cases with a wide range of clinical presentation. The onset of scars is unsual although clinically characteristic of cutaneous sarcoidosis. Most patients with scar sarcoidosis have a systemic disease. It is reported the case of a 65 year-old woman that developed scar nodules 20 years after she had had surgical procedures without systemic manifestations. It is worth mentioning the importance of investigating sarcoidosis with inflammatory alterations in praevia scars.

  4. The practical usefulness of dermoscopy in general dermatology.

    Errichetti, E; Stinco, G


    vasculitis and common inflammatory cicatricial alopecia (discoid lupus erythematosus, lichen planopilaris and folliculitis decalvans). PMID:26086412

  5. Management of delayed intrathoracic esophageal perforation with modified intraluminal esophageal stent.

    Zhou, J-H; Gong, T-Q; Jiang, Y-G; Wang, R-W; Zhao, Y-P; Tan, Q-Y; Ma, Z; Lin, Y-D; Deng, B


    In this article, we reviewed our experience of treatment of the delayed intrathoracic nonmalignant esophageal perforation employing modified intraluminal esophageal stent. Between February 1990 and August 2006, eight patients were included in this study. Five patients experienced sepsis. The interval time between perforation and stent placement ranged from 36 h to 27 days (average, 8.6 days). Esophageal stenting and throracotomy for foreign body removal were performed in four patients. The remaining four patients underwent stent placement and thoracostomy. Nutrition was initiated through gastrostomy after 7 to 10 days after the stenting. The stent was removed after the patients resumed oral intake of food and the esophagogram showed that perforation was closed. There was no death in this group. Signs of sepsis remitted 1 week after stent placement. Complications included stress ulcer, stimulative cough, and pneumonia each. Stent removal ranged 32 to 120 days (average 66.7) after its placement. The stent was kept in place for 4 months to prevent formation of esophageal stricture in one patient with caustic esophageal burns. The follow-up was completed in all the patients. The mean follow-up period was 59 months (range 12-180). One patient with caustic esophageal burn underwent cicatricial esophagectomy and gastric transposition 3 years later due to the esophageal stricture. Barium swallow demonstrated that there was a diverticulum-like outpouching in one patient and slight esophageal stricture at T2 and T3 level in another. One patient developed reflux esophagitis 5 years after stent removal. All the patients finally had a normal intake of food. Modified esophageal stenting is an effective method to manage the delayed intrathoracic esophageal perforation. Prevention of stent migration and its convenient adjustment might be the major advantages of this method. PMID:19191858

  6. [Combined treatment of arteriovenous malformations of the head and neck].

    Galich, S P; Dabizha, A Iu; Gindich, O A; Ogorodnik, Ia P; Al'tman, I V; Gomoliako, I V; Guch, A A


    An arteriovenous malformation (AVM) is a vascular developmental abnormality conditioned by impaired embryonic morphogenesis and characterized by the development of an abnormal connection between arteries and veins. More than 50% of the total number of patients suffering from this pathology are those having the pathological foci localizing in the area of the head and neck. At present, a combined method is both a generally accepted and the most radical one used for treatment for AVM. However, in the majority of cases, excision of the malformation leaves an extensive and complicated defect of tissues, whose direct closure leads to coarse cicatricious deformities. Over the period from 2004 to 2012, we followed up a total of 37 patients presenting with arteriovenous malformations of the head and neck. At admission the patients underwent preoperative examination including clinical tests, ultrasound duplex scanning, arteriography, MRT, and computed tomography. 24-72 hours prior to the operative intervention the patients were subjected to embolisation of the main vessels supplying the vascular malformation. Excision of the AVM was in 8 cases followed by primary closure of the postoperative wound, in 17 patients the defect was closed by transposition of the axial flaps, and 12 subjects underwent free transplantation of composite complexes of tissues. Relapse of the disease was revealed in 17 patients. In the majority of cases, relapses developed during the first year after the operative intervention (10 cases). The control of the disease's course was obtained in 20 patients. In 8 of the 12 patients with free transplantation of flaps we managed to obtain long-term control over the disease's course (more than 5 years). Hence, free microsurgical transplantation of compound complexes of tissues may be considered as a method of choice for closing the defect after excising an AVM in the area of the head and neck. Replacement of the defect with a well-vascularized tissue complex

  7. Endoscopic management of esophageal stenosis in children: New and traditional treatments.

    Dall'Oglio, Luigi; Caldaro, Tamara; Foschia, Francesca; Faraci, Simona; Federici di Abriola, Giovanni; Rea, Francesca; Romeo, Erminia; Torroni, Filippo; Angelino, Giulia; De Angelis, Paola


    Post-esophageal atresia anastomotic strictures and post-corrosive esophagitis are the most frequent types of cicatricial esophageal stricture. Congenital esophageal stenosis has been reported to be a rare but typical disease in children; other pediatric conditions are peptic, eosinophilic esophagitis and dystrophic recessive epidermolysis bullosa strictures. The conservative treatment of esophageal stenosis and strictures (ES) rather than surgery is a well-known strategy for children. Before planning esophageal dilation, the esophageal morphology should be assessed in detail for its length, aspect, number and level, and different conservative strategies should be chosen accordingly. Endoscopic dilators and techniques that involve different adjuvant treatment strategies have been reported and depend on the stricture's etiology, the availability of different tools and the operator's experience and preferences. Balloon and semirigid dilators are the most frequently used tools. No high-quality studies have reported on the differences in the efficacies and rates of complications associated with these two types of dilators. There is no consensus in the literature regarding the frequency of dilations or the diameter that should be achieved. The use of adjuvant treatments has been reported in cases of recalcitrant stenosis or strictures with evidence of dysphagic symptoms. Corticosteroids (either systemically or locally injected), the local application of mitomycin C, diathermy and laser ES sectioning have been reported. Some authors have suggested that stenting can reduce both the number of dilations and the treatment length. In many cases, this strategy is effective when either metallic or plastic stents are utilized. Treatment complications, such esophageal perforations, can be conservatively managed, considering surgery only in cases with severe pleural cavity involvement. In cases of stricture relapse, even if such relapses occur following the execution of well

  8. Influência da vitamina C e da hidrocortisona sobre a tensão anastomótica jejunal em ratos

    Petroianu Andy


    Full Text Available O efeito da vitamina C e da hidrocortisona sobre os processos cicatriciais anastomóticos apresenta resultados conflitantes na literatura. Dessa forma, o presente trabalho teve o objetivo de comparar a resistência cicatricial de anastomoses jejunais em ratos, submetidos à administração de vitamina C e de hidrocortisona, em distintos períodos pós-operatórios. Foram estudados 40 ratos Wistar, submetidos a secção e subseqüente anastomose término-terminal de segmento jejunal, a 10 cm da flexura duodenojejunal. Os animais foram divididos em quatro grupos (n=10: Grupo I - controle; Grupo II - administração de vitamina C oral 100 mg/kg; Grupo III - administração de hidrocortisona intraperitoneal 10 mg/kg; Grupo IV - administração de vitamina C mais hidrocortisona nas doses e vias de administração acima. Avaliaram-se as pressões de ruptura anastomótica nos 5 º e 21º dias pós-operatórios. Os ratos que receberam vitamina C isolada ou associada a hidrocortisona tenderam a uma pressão de ruptura maior do que os demais grupos, tanto no 5º quanto no 21º dias pós-operatórios. Os resultados deste trabalho sugerem que a vitamina C contribui para aumentar a resistência das anastomoses jejunais dos ratos durante os primeiros 5 dias pós-operatórios. A resistência das anastomoses jejunais murinas foi pouco influenciada pela administração de corticóide intraperitoneal.

  9. Lichen planopilaris: Demographic, clinical and histopathological characteristics and treatment outcomes of 25 cases

    Deren Özcan


    Full Text Available Background and Design: Lichen planopilaris (LPP is a type of cicatricial alopecia characterized by autoreactive lymphocytic destruction of the hair follicle. We aimed to evaluate the demographic, clinical and histopathological features, and treatment outcomes of patients with LPP. Materials and Methods: Medical reports of 25 patients, who have been diagnosed with LPP according to the clinical and histopathological findings between January 2006 and June 2012, were retrospectively reviewed. The transverse and vertical sections of scalp biopsy specimens were re-evaluated by a pathologist, and the findings were noted. Results: Of the 25 patients, 18 were female and 7 were male, the mean age was 49.8±12.4 years. Eighteen patients had been diagnosed with classic LPP and 7 patients with frontal fibrosing alopecia (FFA. The alopecia has begun in postmenopausal period in 5 patients with FFA. Alopecia was associated with pruritus, pain and/or burning in 19 patients. Extra-scalp involvement was observed in 11 patients. The most common clinical findings were follicular hyperkeratosis (92%, perifollicular erythema (48%, perifollicular lichenoid papules, and positive hair-pull test (44%. Dermatoscopic examination was performed in 14 patients, and most commonly, absence of follicular openings (100%, perifollicular scales (92.9% and perifollicular erythema (50% were noted. The most common diagnostic histopathological findingsmwere follicular vacuolar and lichenoid degeneration (88% and vacuolar and lichenoid interface changes (56%. Twenty-three patients who were started on treatment received topical, intramuscular and intralesional corticosteroids, topical minoxidil, oral tetracycline, cyclosporine A, and hydroxychloroquine either alone or in combination. Progression of alopecia was prevented and the symptoms and/or signs were reduced in 12 (75% of 16 patients whose follow-up data were available. Conclusion: LPP can be diagnosed accurately through a detailed

  10. Temporal-parietal occipital hair transplantattion on therapy large scarring alopecia%颞顶枕部毛发移植治疗枕部大面积瘢痕性秃发

    王勇; 李兴东; 马文熙


    Objective To explore a treatment after occipital large cicatrial alopecia is more ideal method. Methods Using FUE technique to extract the occipital and planting to the scar at the top of the hair follicles. Results This group of 18 patients,scar tissue after an opera to achieve,postoperative implant hair growed well,scar was overwritten,extremely matural appearance,bothdoctors were satisfied. Conclusions The FUE technology treatment of occipital large cicatricial alopecia operation time,little pain,quick recovery,natural and beautiful appearance,in the caseof occipital resources can be extracted at the top of the hair follicles to grow,the method is worth promoting.%目的:探索一种治疗枕部大面积瘢痕性秃发的更为理想的方法.方法:采用单位毛囊提取术(Follicular Unit Extraction,FUE)提取枕部及颞顶部毛囊种植到瘢痕处.结果:本组18例患者,均经一次手术达到瘢痕修复目的,术后植入的毛发生长良好,瘢痕部位被覆盖,外观极其自然,医患双方均满意.结论:采用FUE技术提取颞、顶、枕部毛囊治疗枕部大面积瘢痕性秃发手术时间短、患者痛苦小、术后恢复快、外观自然美观,该方法值得推广.

  11. Bullous disorders in Konya: A study of 93 cases

    Munise Daye


    Full Text Available Background and Design: Bullous disorders are a group of disorders caused by specific antibodies against tissue adhesion molecules in skin and mucosa or genetic inheritance. There are limited detailed epidemiological studies about bullous disorders in Turkey. In this study we evaluated the demographic, clinical, immunopathologic, course and follow-up properties, cases in Konya. Most of cases were in pemphigus and bullous pemphigoid group, so we gave more information about them. Materials and Methods: Ninty three cases that are followed up during the period of 2006-2012 were included in our study. Results: Female cases were %53,8, male cases were %46,2. The mean age was 52,9. In all of cases; %37,6 cases were pemhigus vulgaris, % 34,4 bullous pemphigoid, %15 herediter epidermolysis bullosa, %4,3 were dermatitis herpetiformis, %4,3 were pemphigus foliaceus, %2,2 pemphigus vegetans, %1,1 Hailey- Hailey disease, %1,1 cicatricial pemphigoid. Mean age of Pemhigus vulgaris onset was 45,9 year and mostly it was observed in females. Pemhigus vulgaris has been started at oral mucosae and the time for diagnosis of pemhigus vulgaris, which began at oral mucosae, was longer than other geogrophic regions. Mean age bullous pemphigoid onset was 70 year and mostly it was observed in females. In % 12,5 of cases disease started at oral mucosae. None of the cases had additional autoimmune disorder. We have treated 25 cases with systemic corticosteriods, 41 cases with systemic corticosteriods and adjuvan agents, 11 cases with only one of adjuvan agents, 16 cases with only local streoids. Conclusions: We report the demographic, clinical, immunopathologic, course and follow-up properties of the bullous disorders in Konya.

  12. Emergency peripartum hysterectomy, physical and mental consequences: a 6-year study

    Denisa-Oana Bălălău


    Full Text Available Emergency peripartum hysterectomy (EPH is performed for massive postpartum hemorrhage following a cesarean delivery or vaginal delivery, in order to save the patient’s life. The current study was performed on a sample of 13.162 patients, which underwent cesarean or vaginal delivery during a period of 6 years, from 2010 to 2015, in Bucur Maternity Hospital. There were two subsequential groups consisting in: 6593 patients with cesarean operations and 6569 patients with vaginal delivery. In 12 cases occurred one or more of the risk factors that lead to EPH, divided equally across the two groups above. The main two types of surgery are a more frequent subtotal hysterectomy, which is the preferred type of EPH as it takes less time and is associated with fewer complications, and a total hysterectomy. The majority of procedures were performed at patients over 35 years old (9 of 12, with a median age of 31,16 (ranging from 21 to 44 years old. The most important risk factor present across the lot was multiparity (11 from 12, with cicatricial uterus being the second one (4 of 12. ICU median time was 4,5 days (ranging from 3 to 15 days, with a median blood transfusion necessity of around 2,4 I.U per patient. There were no mother or newborn reported deaths, neither PTSD following EPH.EPH is a procedure performed as last-resort, life-saving surgery, leaving no time for mental preparation of the patients. This may predispose to negative psychological outcomes, especially because they are not part of decision-making process due to the emergency character of hysterectomy.

  13. Interferón alfa-2b tópico como primera opción en las neoplasias intraepiteliales corneoconjuntivales Topical interferon alfa-2b for primary treatment of conjunctiva-cornea intraepithelial neoplasia

    M. Pérez de Arcelus


    Full Text Available Se describen dos casos de neoplasia intraepitlelial corneo-conjuntival (CIN tratados con interferón alfa-2b (IFN alfa-2b tópico como primera elección. El tratamiento clásico de los CIN ha sido tradicionalmente la resección completa con márgenes de seguridad seguida de crioterapia en el lecho quirúrgico. No obstante, y puesto que la tasa de recidivas puede alcanzar el 50% han sido propuestos coadyuvantes como la mitomicina C y el 5 fluoracilo, con el consiguiente riesgo de toxicidad corneal y límbica. El IFN alfa-2b presenta una eficacia similar a la cirugía en la erradicación completa de la masa tumoral como primera opción, con escasos efectos secundarios y nulo potencial carcinogénico, incluso en casos de recurrencia a terapia con mitomicina C, lesiones quirúrgicas residuales y formas difusas.We describe two cases of conjunctival-cornea intraepithelial neoplasia (CIN, treated with topical IFN alfa 2b. The traditional treatment for CIN is surgical excision usually with adjunctive cryotherapy. However, residual tumour may remain, which can lead to recurrence rates of more than 50%. 5-Fluorouracil, mitomicyn C and interferon alfa 2b are new pharmacological agents that have proved their efficacy in the treatment of CIN. As side effects are common, we present IFN alfa 2b as a single therapeutic agent as an effective and optimal treatment for presumed recurrent corneal and conjunctival intraepithelial neoplasia. It offers the benefits of topical therapy and avoids the risks of surgical or other interventions - specifically, ocular surface toxicity, cicatricial conjunctival changes, and limbal stem cell deficiency.

  14. Treatment of frontal fibrosing alopecia and lichen planopilaris: a systematic review.

    Rácz, E; Gho, C; Moorman, P W; Noordhoek Hegt, V; Neumann, H A M


    Frontal fibrosing alopecia (FFA) is a primary lymphocytic cicatricial alopecia with characteristic clinical pattern of progressive frontotemporal hairline recession, perifollicular erythema and hyperkeratosis and symptoms of itch and burning, occurring mainly in post-menopausal women. FFA is considered a subtype of lichen planopilaris (LPP), based on their identical histopathology. Currently, no evidence-based treatment is available for FFA. Our aim was to determine the effectiveness of available treatment options for FFA, and to identify promising treatment options for future studies. For this, literature search was conducted to find all primary studies on the treatment of FFA and LPP. From the primary studies, data were subtracted and analysed. No randomized controlled trials were found, and one controlled trial. Treatment of 114 patients is described in the literature. They received 10 different regimes, of which oral 5-alpha-reductase inhibitors were provided most often, resulting in good clinical response in 45% of them. Hydroxychloroquine resulted in good clinical response in 30% of the 29 treated patients. Topical corticosteroid preparations are ineffective in FFA. The remaining treatments were all reported in less than 10 patients. For the treatment of LPP, topical corticosteroid preparations are the first line of treatment, followed by oral cyclosporine and systemic corticosteroids, although they are characterized by a high relapse rate. Summarizing, there is currently no effective treatment of FFA, the most effective being oral 5-alpha-reductase inhibitors that possibly affect the accompanying androgenetic alopecia. We argue that oral cyclosporine A might be a good candidate for future studies on the treatment of FFA. PMID:23531029

  15. CT findings of mucoid impaction in 13 case: Speculation on their etiology

    Mucoid impaction presents a typical appearance on plain radiography and CT. If the lesion is multiple with manifestations of diffuse bronchopathy, it usually is a medical disease. On the other hand, if the lesion is single, an effort should be made to determine the underlying disease. The most frequent cause of the latter lesion is lung cancer, which generally can be depicted by CT. We retrospectively reviewed 13 cases of chest CT with obstructive mucoid impaction, in which CT and BFS(bronchofiberscopy) did not reveal a specific underlying cause. Women in the twenties and thirties were dominant and a past history of pulmonary tuberculosis was present in nine, BFS, done in nine, showed bronchial obstruction, and also luminal narrowing and cicatricial mucosal changes proximal to the obstruction in all them. Endobronchial tuberculosis was proven in only one case by bronchial wall biopsy. Lobectomy was performed in three. At the bronchial obstruction site, thickened bronchial walls were connected to proximal bronchi and there was no evidence of a clear underlying pathology except submucosal fibrosis in all three. The level of bronchial obstruction , determined by CT and BFS, was in the main bronchus in one, lobar bronchi in six, and divisional or segmental bronchi in seven, CT additionally showed calcific spots in their parenchyma connected to the bronchial obstruction in seven cases, and other changes suggestive of tuberculous origin in eight patients. In the context of the clinical history, BFS results, CT findings and pathologic findings of surgical specimen, most, if not all, of these 13 lesions are supported to be a sequela of endobronchial tuberculosis

  16. Uso tópico de ketanserina na cicatrização de feridas cutâneas induzidas em equinos Topical use of kentaserin on healing of experimentally-induced skin wounds in horses

    G. Ribeiro


    Full Text Available Estudou-se a eficácia do uso tópico de ketanserina como promotor da cicatrização de feridas cutâneas induzidas em equinos. As feridas foram produzidas em ambos os metâmeros na região da garupa de oito cavalos adultos, em formato quadrangular, medindo cinco centímetros de lado. Aleatoriamente um dos metâmeros foi escolhido como tratado, permanecendo o contralateral como controle. As lesões do grupocontrole foram lavadas somente com água e detergente neutro e no grupo tratado foi realizado o mesmo procedimento seguido de aplicação tópica de ketanserina. A evolução macroscópica e microscópica do processo cicatricial foi avaliada e a área de cada ferida determinada no decorrer do período experimental. Não foram verificadas diferenças significativas entre as feridas do grupo-controle e do grupo tratado com ketanserina durante 56 dias de avaliação.The efficiency of topical use of kentaserin on healing evolution of induced skin wounds in horses was studied. The experimental surgical wounds were made in both sides of the croup of eight adult horses. The wounds were square-shaped measuring 5cm of each side. One side was considered control and the opposite one treated. Both control and treated animals were rinsed with water and neutral soap. Additionally, the wound on the treated horses received the topical treatment with ketanserin. Macroscopic and microscopic healing evolution of the wounds was evaluated and their areas were determined during the experimental period. The use of topical ketanserin showed no difference between the control and the treated wounds during a period of 56 days.

  17. An advanced rectal carcinoma treated with radical radiotherapy using a proton beam

    Miyo, Yasushi; Koyama, Shohei; Kobayashi, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Clinical Medicine) (and others)


    The authors present the case of a 72-year-old man admitted to hospital because of anal bleeding. A barium enema and subsequent endoscopic studies revealed a Borrmann type 3, well-differentiated adenocarcinoma of the rectum, with marked narrowing at the Rb to Ra region. On the basis of CT scans, a metastasis to the regional lymph nodes and the softer fatty tissue in the pelvic space was suspected. As surgery was contraindicated since an abnormal antibody against the erythrocytes was detected in the patient's blood, radical radiotherapy was pursued to cure the rectal cancer. Thus, a 250 MeV proton beam was administered to the primary lesion, and a total dose of 90 Gy was delivered in 28 fractions of 3 to 4 Gy daily over 64 days. In addition, X-ray therapy, using a 12 MV linear accelerator and a total dose of 50.4 Gy in 28 fractions of 1.8 Gy, was used for the treatment of a regional lymph node metastasis in the pelvic space. Three years and five months later, the patient died of an occlusive ileus of the rectum. On autopsy, the rectum revealed organized cicatricial fibrosis with marked stenosis. Further, the histological findings of the irradiated tumor cells showed massive fibrosis and hyalinized connective tissue. Vital cancer cells only were found in small parts of the primary rectal lesion, and the biological significance of these small clusters of vital tumor cells is discussed. Finally, neither a regional lymph node metastasis nor a distant metastasis, i.e., to the liver and/or lung, were observed. In conclusion, proton beam therapy was seen to have a beneficial effect on this rectal cancer and on the local control of the disease. (author).

  18. The role of PPARγ-mediated signalling in skin biology and pathology: new targets and opportunities for clinical dermatology.

    Ramot, Yuval; Mastrofrancesco, Arianna; Camera, Emanuela; Desreumaux, Pierre; Paus, Ralf; Picardo, Mauro


    Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that modulate the expression of multiple different genes involved in the regulation of lipid, glucose and amino acid metabolism. PPARs and cognate ligands also regulate important cellular functions, including cell proliferation and differentiation, as well as inflammatory responses. This includes a role in mediating skin and pilosebaceous unit homoeostasis: PPARs appear to be essential for maintaining skin barrier permeability, inhibit keratinocyte cell growth, promote keratinocyte terminal differentiation and regulate skin inflammation. They also may have protective effects on human hair follicle (HFs) epithelial stem cells, while defects in PPARγ-mediated signalling may promote the death of these stem cells and thus facilitate the development of cicatricial alopecia (lichen planopilaris). Overall, however, selected PPARγ modulators appear to act as hair growth inhibitors that reduce the proliferation and promote apoptosis of hair matrix keratinocytes. The fact that commonly prescribed PPARγ-modulatory drugs of the thiazolidine-2,4-dione class can exhibit a battery of adverse cutaneous effects underscores the importance of distinguishing beneficial from clinically undesired cutaneous activities of PPARγ ligands and to better understand on the molecular level how PPARγ-regulated cutaneous lipid metabolism and PPARγ-mediated signalling impact on human skin physiology and pathology. Surely, the therapeutic potential that endogenous and exogenous PPARγ modulators may possess in selected skin diseases, ranging from chronic inflammatory hyperproliferative dermatoses like psoriasis and atopic dermatitis, via scarring alopecia and acne can only be harnessed if the complexities of PPARγ signalling in human skin and its appendages are systematically dissected. PMID:25644500

  19. Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: first clinical application.

    Lange-Consiglio, A; Spelta, C; Garlappi, R; Luini, M; Cremonesi, F


    Bovine udder infections induce a variety of changes in gene expression of different growth factors that may suggest their possible role in glandular tissue protection or repair processes. Growth factors and also chemokines and cytokines may act synergistically to increase the infiltration of neutrophils and macrophages to promote angiogenesis, fibroplasia, matrix deposition, and, ultimately, re-epithelialization. Considering the vast applications, typically in human medicine, of platelet concentrate (PC) and its ease of preparation, the aim of our study was to evaluate an alternative therapy to stimulate the regeneration of glandular tissue, administering a concentration in excess of the growth factors contained in the PC. In each one of the 3 farms examined in the trial, PC was prepared from donor cows in good health, free from infections, and with no records of medications administered during the previous 2 mo. The platelet produced in one farm was used only for treating the cows of the same farm in a heterologous way. A total of 229 mastitic quarters were divided in 3 groups: antibiotic group (treated with intramammary antibiotic), antibiotic and PC group (treated intramammarily with antibiotics in association with PC), and PC group (treated with intramammary PC alone). The diagnosis of mastitis was based on somatic cell count and bacteriological evaluation of the milk from the affected quarter. Platelet concentrate, alone or in association with antibiotic, was used for 3 consecutive days as an unconventional therapy in bovine acute and chronic mastitis. Our data show that the associated action of antibiotic and PC performed significantly better than the antibiotic alone, either for the recovery of the affected mammary quarters or for somatic cell count reduction. In the same way, the association antibiotic plus PC showed significantly fewer relapses compared with the antibiotic alone, either for acute or chronic mastitis. The treatment with only PC did not show

  20. Aflatoxicose em cães na região Sul do Rio Grande do Sul

    Angelita dos Reis Gomes


    Full Text Available Descrevem-se os aspectos clinicopatológicos de casos de aflatoxicose em cães no Sul do Rio Grande do Sul. Foi realizado um estudo retrospectivo dos casos diagnosticados como aflatoxicose em cães necropsiados no Laboratório Regional de Diagnóstico (LRD da Faculdade de Veterinária da Universidade Federal de Pelotas (UFPel no período de 1978 a 2012. Em quatro casos o diagnóstico foi confirmado pela detecção de níveis de 89 a 191 ppb de aflatoxinas B1 e G1 no alimento dos cães. De um total de 27 cães com cirrose hepática, em seis havia suspeita de aflatoxicose pelas lesões macro e microscópicas e pelo tipo de alimentação que os cães recebiam. Os sinais clínicos nos casos confirmados e nos suspeitos caracterizaram-se por apatia, diarreia, icterícia e ascite, com evolução para morte em 8 a 30 dias nos casos confirmados e em 15 a 60 dias nos casos suspeitos. A dieta era à base de derivados de milho ou arroz, farelo de amendoim e, em um caso suspeito, a dieta era ração comercial. As alterações macroscópicas caracterizaram-se por ascite, icterícia, fígado aumentado de tamanho, com ou sem nódulos, hemorragia nas serosas, conteúdo intestinal hemorrágico. Os casos foram classificados de acordo com o padrão histológico principal, caracterizado por vacuolização difusa no citoplasma de hepatócitos nos casos agudos, por proliferação de ductos biliares e discreta fibroplasia nos casos subagudos e por fibrose acentuada nos casos crônicos. Aparentemente, a enfermidade não é importante como causa de morte em cães na região, no entanto, alerta-se para a possibilidade de casos com diagnóstico de cirrose hepática sem causa determinada serem causados por aflatoxicose.

  1. Fibromuscular dysplasia

    Jeunemaitre Xavier


    Full Text Available Abstract Fibromuscular dysplasia (FMD, formerly called fibromuscular fibroplasia, is a group of nonatherosclerotic, noninflammatory arterial diseases that most commonly involve the renal and carotid arteries. The prevalence of symptomatic renal artery FMD is about 4/1000 and the prevalence of cervicocranial FMD is probably half that. Histological classification discriminates three main subtypes, intimal, medial and perimedial, which may be associated in a single patient. Angiographic classification includes the multifocal type, with multiple stenoses and the 'string-of-beads' appearance that is related to medial FMD, and tubular and focal types, which are not clearly related to specific histological lesions. Renovascular hypertension is the most common manifestation of renal artery FMD. Multifocal stenoses with the 'string-of-beads' appearance are observed at angiography in more than 80% of cases, mostly in women aged between 30 and 50 years; they generally involve the middle and distal two-thirds of the main renal artery and in some case also renal artery branches. Cervicocranial FMD can be complicated by dissection with headache, Horner's syndrome or stroke, or can be associated with intracerebral aneurysms with a risk of subarachnoid or intracerebral hemorrhage. The etiology of FMD is unknown, although various hormonal and mechanical factors have been suggested. Subclinical lesions are found at arterial sites distant from the stenotic arteries, and this suggests that FMD is a systemic arterial disease. It appears to be familial in 10% of cases. Noninvasive diagnostic tests include, in increasing order of accuracy, ultrasonography, magnetic resonance angiography and computed tomography angiography. The gold standard for diagnosing FMD is catheter angiography, but this invasive procedure is only used for patients in whom it is clinically pertinent to proceed with revascularization during the same procedure. Differential diagnosis include

  2. Intralesional polysulphated glycosaminoglycan as treatment of equine collagenase induced tendinitis: clinical, ultrasonographic and histopathologic evaluation Polissulfato de glicosaminoglicanas no tratamento intralesional de tendinite induzida em eqüinos: avaliação clínica, ultra-sonográfica e histopatológica

    S. Marxen


    Full Text Available The effect of intratendineous injections of polysulphated glycosaminoglycan (PSGAG as treatment of collagenase-induced tendonitis was studied. Two groups (GI and GII of five Arabian horses each, males and females, two to six year-old, were submitted to experimental tendinitis of the superficial digital flexor tendon of the left thoracic limb by intratendineous injection of 1.0ml of collagenase (2.5mg/ml. Seven days after the induced-lesions were created, the horses of GI received five intralesional injections of 1.0ml (125mg of PSGAG, every four days. Horses of GII received injections of saline in the same dose and rate. Clinical and ultrasonographic evaluations were performed periodically, during 150 days. All animals showed lameness, increased local pain, heat and swelling 24 hours after the injury was created. All signs, except to swelling, which remained visible by the end of the study, showed regression in all animals. Lesions of variable size, shape and position were evidenced by the ultrasonographic evaluation, reaching maximum severity between the seventh and the 23rd days. By the end of the study, the echogenicity grade ranged from 1 to 2, and the grade of fiber alignment from 0 to 2. The histopathologic analysis demonstrated repair areas with intense fibroplasia and neovascularization, collagen fibers poorly organized, and thickened hypercellular endotenon. The data of this study did not show significant differences between the treated and control groups, therefore leading to the conclusion that the intralesional injection of PSGAG did not have beneficial effects in the treatment of collagenase-induced tendinitis.Estudou-se o efeito de aplicações intratendíneas do polissulfato de glicosaminoglicanas (PSGAG no tratamento de tendinite induzida pela colagenase. Dois grupos (GI e GII de cinco eqüinos da raça Puro-Sangue Árabe, machos e fêmeas, com idades entre dois e seis anos, foram submetidos à tendinite do tendão flexor digital

  3. Healing activity of laser InGaAlP (660nm in rats Atividade cicatrizante do laser AlGaInP (660nm em ratos

    Kelly Cristina Borges Tacon


    Full Text Available PURPOSE: To evaluate the effect the healing activity of diode laser Indium Gallium Aluminum Phosphorus (InGaAlP ë660nm on healing of surgical wounds in rats. METHODS: Fifty-four female Wistar rats were used, divided into three groups (n=18 and subdivided into three subgroups (n=6 to be studied in 5, 10 and 15th days after surgical procedures. The wound was induced in the dorsal-cervical using punch. The lesions were irradiated on alternate days with InGaAlP laser, the energy densities of 3J/cm² (L3 or 6J/cm² (L6. The control group received no irradiation. At 5, 10 and 15th days after surgery the animals were euthanized and the repair area was removed and histological sections were stained with hematoxylin-eosin and picrossírius. We evaluated macroscopic and histological lesions in the times cited, as well as morphometric analysis of angiogenesis and collagen content. RESULTS: The wound healing activity InGaAlP laser was evidenced by increased angiogenesis group L3 and L6 in relation to control group (CG at the 5th day (p=0.0001 and decreased polymorphonuclear infiltrate and hemorrhage (p=0.045 and p=0.07 respectively in the groups L3 and L6 in relation to control group (GC. On the 10 and 15th days was also observed in groups treated with laser L3 and L6 stimulation was pronounced fibroplasia (p=0.0003 and p=0.034 respectively when compared with the control group (CG. CONCLUSION: The InGaAlP laser acted positively on the healing of skin wounds in rats.OBJETIVO: Avaliar a atividade cicatrizante do laser diodo Alumínio Gálio Índio Fósforo (AlGaInP, ë660nm em feridas cutâneas induzida em ratos. MÉTODOS: Foram utilizados 54 ratos Wistar, fêmeas, divididos em três grupos (n=18 e subdivididos em três subgrupos (n=6 animais para serem estudados no 5, 10 e 15º dias após o procedimento cirúrgico. A ferida foi induzida em região dorso-cervical utilizando punch. As lesões foram irradiadas em dias alternados com laser AlGaInP, nas

  4. Preventive effects of chitosan on peritoneal adhesion in rats

    Zhi-Liang Zhang; Si-Wei Xu; Xie-Lai Zhou


    AIM: To study the effects of chitosan gel and blending chiston/gelatin film on preventing peritoneal adhesion in rats.METHODS: SD rats were randomly divided into 2groups, group A treated with chitosan gel and group B with blending chiston/gelatin film. In group A, rats were randomly subdivided into 3 subgroups as groups A1,A2 and A3, and different methods were used to induce peritoneal adhesions at the dead end of vermiform process in each group as follows: Group A1 with trauma,A2 with talc powder and A3 with ligation of blood vessel.In each subgroup, rats were redivided into control group and experimental group whose treated vermiform processes were respectively coated with chitosan gel and normal saline immediately after the adhesioninduced treatments. In group B, all the rats received traumatic adhesion-induced treatments and then were randomly divided into 4 groups (groups B1, B2, B3, B4).Group B1 served as control group and were coated with normal saline in the vermiform processes immediately after the treatments, and groups B2, B3 and B4 with 100% chitosan film, chitosan film containing 10% gelatin and chiston film containing 50% gelatin, respectively.At 2 and 4 wk after the above treatments, half of the rats in each terminal group were belly opened, and the peritoneal adhesive situation was graded and histopathological changes were examined.RESULTS: (1) In group A, regarding peritoneal adhesion situation: At both 2 and 4 wk after the treatments, for groups A1 and A3, the adhesive grades of experimental groups were significantly lower than those of the control group (2 wk:H=4.305, P<0.05 for A1, H = 6.743, P< 0.01 for A3; 4 wk:H= 4.459, P < 0.05 for A1,H=4.493, P < 0.05 for A3). However, of group A2, there was no significant difference between the experimental and control groups (2 wk:H= 0.147, P > 0.05; 4 wk:H=1.240, P>0.05). Regarding pathological changes:In groups A1 and A3, the main pathological change was fibroplasia. In group A2, the main

  5. Carcinomas basocelulares: estudo clínico e anatomopatológico de 704 tumores Basal cell carcinomas: anatomopathological and clinical study of 704 tumors

    Aurilene Monteiro Bandeira


    Full Text Available FUNDAMENTOS: Este é um estudo retrospectivo e anatomopatológico de 704 carcinomas basocelulares de 623 pacientes, diagnosticados no período de 1991 a 1996, no setor de Dermatopatologia da Clínica Dermatológica do Hospital das Clínicas da UFPE e em um laboratório privado de dermatopatologia da cidade do Recife. OBJETIVOS: Caracterizar aspectos clínicos e anatomopatológicos dos carcinomas basocelulares diagnosticados nos dois serviços da região de Pernambuco. MÉTODOS: Para o estudo clínico, os dados foram retirados dos prontuários e para o estudo anatomopatológico, macro e microscópicos, foram feitas revisão dos preparados histológicos. Para o crescimento vertical empregaram-se métodos baseados nas técnicas histoprognósticas de Clark e Breslow, aplicadas ao melanoma maligno. RESULTADOS: Clínicos: a maior incidência foi no sexo feminino (55,7%, faixa etária de 55 a 72 anos, tempo de evolução com variabilidade elevadíssima, desde um mês a 40 anos, e a cabeça foi a região topográfica mais freqüente (73,8%, principalmente nasal (21,1% e zigomática (18,5%. A forma nodular pigmentada (47,4% foi a mais encontrada e o tamanho das lesões independeu do tempo evolutivo. Histologicamente os padrões considerados baseados apenas nos arranjos parenquimais, foram os adenóide, compacto, fibroepitelial de Pinkus plexiforme, pseudocístico, reticulado, superficial e tricoepitelial, predominando o adenóide (28,3%. A média de crescimento foi em 2/3 da derme reticular (32,4%, e os tumores que mais se aprofundaram mostraram fibroplasia intensa. Houve concomitância de vários tipos celulares em um mesmo tumor e o pigmento melânico esteve mais presente nos tricoepitelioides. CONCLUSÃO: A fundamental importância da caracterização clínica e anatomopatológica dos carcinomas basocelulares destes serviços, sem diferenças muito significativas para os grupos e atenção para definições comportamentais e proposições ao relat

  6. Fixação esquelética externa tipo Ia (unilateral-uniplanar para osteossíntese diafisária de úmero em pombos domésticos (Columba livia Type Ia (unilateral-uniplanar external Skeletal fixation for treatment of humeral diaphysis fracture in domestic pigeons (Columba livia

    Alessandro Moraes Leotte


    Full Text Available Das várias patologias que podem acometer as aves, são mais freqüentes as fraturas de ossos longos das asas e dos membros pélvicos. Este experimento teve como objetivo observar, por meio de exames clínicos, histológicos e radiográficos, a resposta cicatricial óssea ao uso do fixador externo tipo Ia (unilateral-uniplanar na redução aberta de fratura diafisária, de úmero em pombos domésticos (Columba livia. Foram estudados 14 pombos com peso variando entre 290 e 420 gramas. A fratura foi realizada na diáfise do úmero direito e, logo após, estabilizada com aparelho de fixação externa tipo Ia, utilizando-se, como barra de fixação, o acrílico autopolimerizável. O tempo médio para o completo desaparecimento da linha de fratura foi de 28±6,44 dias e para formação de calo ósseo, 17±3,26 dias. As aves, mesmo com o aparelho de fixação, retornaram ao vôo aos 28±1,2 dias, exceto uma que não recuperou a capacidade de vôo. A técnica de fixação externa demonstrou ser eficaz para o tratamento de fraturas diafisárias de úmero em pombos domésticos.Among the various pathologies that can inflict birds, the long bones fractures of the wings, and pelvic members are more commom. Fourteeen domestic pigeons (Columba livia weighing 290 to 420g, were submitted to open reduction of humeral diaphysis fracture with external fixator type Ia (unilateral-uniplanar in order to evaluate the clinical, histological and radiographic evolution of the bone healing. The external fixator device was stabilized with methilmetacrylate acrylic. The average time necessary to the complete disappearance of the fracture line by radiographic evaluation was 28±6.44 days. The bone callus was identified at 17±3.26 postoperative days. The birds returned to flying in 28±1.2 days yet with the fixation device and only one did not recovere the flying abillity. The external fixation technique is an efficient method to treat diaphiseal humeral fracture in domestic

  7. Avaliação dos fatores de risco no linfedema pós-tratamento de câncer de mama Risk factors for breast cancer related lymphedema

    Laura Ferreira de Rezende


    Full Text Available A principal complicação tardia no pós-operatório de câncer de mama é o desenvolvimento do linfedema, uma doença crônica, progressiva, geralmente incurável. O aumento do volume do membro pode desfigurar a imagem corporal, assim como aumentar a morbidade física e psicológica da paciente, além de promover significativo prejuízo para as funções. O presente estudo foi desenvolvido por meio de uma revisão sistemática a partir do cruzamento aleatório das palavras-chave: "linfedema", "compensações linfáticas", "sistema linfático", "dissecção axilar", "fatores de risco" e "câncer de mama". Foram selecionados 18 artigos entre os anos de 1979 e 2009, nos quais foram encontrados como principais fatores de risco para o desenvolvimento do linfedema a radioterapia, radioterapia axilar, infecção, dissecção axilar seguida de radioterapia, obesidade, número de linfonodos retirados e comprometidos e agressividade da cirurgia. As formas de compensação linfática após a dissecção axilar, como as anastomoses linfo-linfáticas, podem ser prejudicadas pela formação cicatricial, seroma pós-operatório, radioterapia e exercícios inadequados para reabilitação de ombro no câncer de mama.The main late complication after the surgery of breast cancer is the development of lymphedema, a chronic, progressive, usually incurable disease. The increase in the volume of the limb can disfigure the body image and develop the physical and psychological morbidity of the patient, promoting significant damage to the functions. This study was developed through a systematic review from the randomized crosschecking of the keywords "lymphedema", "lymphatic compensation", "lymphatic system", "axillary dissection", "risk factors" and "breast cancer". Eighteen articles were selected, between 1979 and 2009, in which radiotherapy, axillary radiation, infection, axillary dissection followed by radiotherapy, obesity, number of removed and impaired lymph

  8. [Ocular surface reconstruction by tissue engineering].

    Kinoshita, Shigeru


    Ocular surface reconstruction by tissue engineering using somatic stem cells is a second-generation modality. In order to treat bilaterally affected, severe ocular surface disorders, we investigated the transplantation of two types of cultivated mucosal epithelia: allogenic corneal epithelial stem cells, and autologous oral mucosal epithelial cells. For this, first, we summarized the clinical results of allogenic keratoepithelioplasty and limbal transplantation. In addition, we showed that the immunological shift from Th1 to Th2 by using keyhole limpet hemocyanin was effective in suppressing the incidence of immunological rejection. Second, we investigated the transplantation of cultivated human corneal epithelial stem cells onto amniotic membrane. The cultivated sheet was created by co-culture with 3T3 fibroblasts, using the air-lift method, in cultivating the corneal epithelial stem cell on the amniotic membrane. These cultivated cells demonstrated positive keratin 3 and 12 specific to in vivo corneal epithelium, tight junction related proteins, and telomerase activity. The transplanted allogenic human corneal epithelial sheet survived on the corneal surface in all cases, and was quite effective for achieving ocular surface stability in the acute phase of Stevens-Johnson syndrome, ocular cicatricial pemphigoid, or chemical injury. However, a few cases developed immunological rejection or opportunistic infection. Third, to establish the transplantation of the autologous cultivated oral mucosal epithelial sheet, we performed animal experiments using rabbits. In vitro oral mucosal epithelial sheet showed histology similar to that of in vivo corneal epithelial sheet. It expressed positive keratin 3 as well. Since the autologous transplantation of this sheet survived on the ocular surface with the recovery of corneal transparency, a cultivated oral mucosal epithelium may become a substitute for corneal epithelium. Fourth, we created a cultivated human corneal

  9. Prevalence of anti-gliadin antibody in patients with alopecia areata: a case-control study

    Hallaji Z


    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 st1":*{behavior:url(#ieooui } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Alopecia areata, a non-cicatricial form of hair loss, is believed to be an immunologic response that targets hair follicles. Genetic background is important in the pathogenesis of this disorder, although some evidence point to the role of melanocytic antigens. There are some reports on the relationship between alopecia areata and celiac disease. The aim of the present study was to identify antigliadin antibodies in patients with alopecia areata. "n"nMethods: Fifty patients, aged 2.5-50 years, with alopecia areata presenting to the dermatology clinic of Razi Educational Hospital in Tehran, Iran, and fifty healthy individuals, aged 5-48 were matched and enrolled in the study. After signing an informed consent form, blood samples (10 ml clotted blood were obtained from the participants and sent to referral laboratory for the presence of antigliadin IgA and IgG antibodies. Concentrations of antibodies were measured by ELISA through a full automatic ELISA reader. The data were analyzed statistically."n"nResults: The study included 29(58% male and 21(42% female patients with a mean age of 24.6 years. The control group included 29(58% male and 21(42% female individuals with a mean age

  10. Efeito cicatrizante e atividade antibacteriana da Calendula officinalis L. cultivada no Brasil Healing effect and antibacterial activity of Calendula officinalis L. cultivated in Brazil

    L.M.L. Parente


    Full Text Available Desde a antiguidade propriedades medicinais são atribuídas às flores da Calendula officinalis L. (Asteraceae destacando-se a atividade cicatrizante. Estudos sobre a atividade geral de plantas medicinais na cicatrização vêm sendo realizados, sem especificar sobre qual das fases da cicatrização a planta atua. Neste trabalho a atividade cicatrizante e antiinflamatória do extrato etanólico das flores da C. officinalis cultivada no Brasil foi avaliada em feridas cutâneas de ratos Wistar, por meio de avaliação macroscópica e histológica. A atividade antimicrobiana do extrato e das frações hexânica e diclorometano também foi avaliada. A atividade antiinflamatória do extrato etanólico da calêndula foi atribuída à diminuição da exsudação serosa, da hiperemia, da deposição de fibrina e da hiperplasia epidermal, além de resultar em crostas mais delgadas e umedecidas. Observaram-se também aumento de colágeno no tecido de granulação e efeito antibacteriano. Assim, o extrato etanólico da calêndula atuou de forma positiva sobre a atividade cicatricial em feridas cutâneas de ratos, bem como apresentou atividade antibacteriana in vitro.Since ancient history medicinal properties are attributed to flowers of Calendula officinalis L. (Asteraceae, mainly concerning its healing activity. Studies about the general activity of medicinal plants in healing wounds have been conducted without specifying in which healing phase the plant acts. In this work, the anti-inflammatory and healing activity of the ethanolic extract of C. officinalis flowers cultured in Brazil was evaluated in cutaneous wounds of Wistar rats through macroscopic and histological evaluation. The antibacterial activity of the extract and dichloromethane and hexane fractions was also evaluated. The anti-inflammatory activity of calendula ethanolic extract led to a reduction in plasmatic exudation, hyperemia, fibrin deposition and epidermal hyperplasia, besides

  11. Lichen planopilaris is characterized by immune privilege collapse of the hair follicle's epithelial stem cell niche.

    Harries, Matthew J; Meyer, Katja; Chaudhry, Iskander; E Kloepper, Jennifer; Poblet, Enrique; Griffiths, Christopher Em; Paus, Ralf


    this highly treatment-resistant cicatricial alopecia. PMID:23788005

  12. Técnicas de sutura do tubo digestivo em plano único com nós atados no lume, em cães: pontos simples totais versus pontos extramucosos

    Azevedo João Luiz Moreira Coutinho


    Full Text Available OBJETIVO: Comparar a anastomose do tubo digestivo em plano único com nós atados no lume por sutura com pontos totais versus pontos extramucosos. MÉTODOS: Foram operados seis cães, com realização de duas secções transversas do jejuno a 30 cm e a 70 cm da flexura duodenojejunal e sutura, na face posterior com pontos extramucosos atados sobre a submucosa, e na face anterior com pontos totais atados sobre a mucosa. No 7º PO foram avaliadas, na face posterior, as aderências na linha de sutura e feitos exames macroscópico e microscópico. RESULTADOS: As aderências peritoneais foram mais profusas nas suturas extramucosas com tecido aderencial sobre a linha de sutura, sem reconstituição da serosa, ou com a deformidade cicatricial das serosas dos cotos angulando a anastomose. A serosa teve boa reconstituição nas suturas totais. O epitélio mucoso reconstituiu-se perfeitamente nas extramucosas, mas não nas totais. Nas suturas totais houve focos residuais de inflamação aguda.O realinhamento, a reestruturação e a regeneração das camadas (exceto a serosa, cuja regeneração foi prejudicada por aderências foi melhor na sutura extramucosa que na total. A muscular da mucosa não se regenerou em nenhuma anastomose. Os polimorfonucleares, os macrófagos, os fibroblastos e as fibras colágenas foram mais numerosos (significância estatística na sutura total. CONCLUSÃO: As suturas totais da parede posterior da anastomose com nós atados no lume, sobre a mucosa, são seguras, apesar da inflamação maior. A sutura extramucosa da parede posterior, com nós atados no lume, sobre a submucosa, propicia a formação de aderências peritoneais, devendo ser evitada.

  13. Diagnostic and interventional radiology in the post-operative period and follow-up of patients after rectal resection with coloanal anastomosis

    was cured in 1 week. In 36 cases of cicatricial stenosis, 17 at the coloanal anastomosis and 19 at the pouch, radiological examination always detected the lesion, correctly defining its anatomical characteristics, nature and extension. Of the 19 cases of stenosis treated radiologically, 15 recovered an adequate intestinal calibre for normal evacuation. During follow-up of the 175 patients operated on, 21 cases of recurrence were detected. Radiological examination was requested as the first investigation in only one of these cases, for a patient with subocclusion. Radiological investigations in patients who have undergone coloanal anastomosis are of real diagnostic value in the immediate post-operative period, during closure of the protective colostomy and in the follow-up of symptomatic recanalized patients. (orig.)

  14. Expansión cutánea externa con endermología: un método alternativo complementario en reconstrucción mamaria diferida

    M. De la Cruz


    Full Text Available Con el objetivo de mejorar las técnicas de reconstrucción mamaria diferida, incorporamos la técnica de endermología como método de expansión cutánea externa para eliminar el plano cicatricial inextensible que se forma entre la fascia del pectoral y el tejido celular subcutáneo tras la cirugía radical. Se trata de un procedimiento sencillo, de bajo coste y de aplicación universal, que en estos casos enfocamos a evitar nuevas cicatrices, complicaciones de zonas donantes, ingresos hospitalarios prolongados y los riesgos quirúrgicos elevados propios de las técnicas de reconstrucción mamaria con colgajos libres o pediculados, así como los frecuentes resultados insatisfactorios de la reconstrucción con expansor y prótesis en pacientes radiadas. Cuando la piel presenta un grosor insuficiente o la paciente ha sido sometida a radioterapia previa, complementamos el tratamiento con lipotransferencia de grasa abdominal. Todos los tratamientos de expansión cutánea externa se realizan en consulta mediante un equipo Cellu m6 (LPG® endermologia, Francia. Tras la expansión, realizamos la reconstrucción como un aumento mamario simple con prótesis anatómica. Medimos la ganancia de elasticidad cutánea tras masaje y el grosor dermograso mediante test de pellizco. Realizamos la simetrización de la mama contralateral ptósica o hipertrófica en el momento de la colocación del implante o de la mastectomía subcutánea contralateral profiláctica en pacientes de riesgo. Incluimos en el estudio mujeres sin distinción de edad, tipo o tamaño de tumor, o si han recibido radio o quimioterapia. Con esta técnica, obtenemos resultados homogeneamente satisfactorios sin añadir nuevas cicatrices y mejorando la calidad de la piel y de la cicatriz de mastectomía.

  15. Experimental study on operative methods of pancreaticojejunostomy with reference to anastomotic patency and postoperative pancreatic exocrine function

    Ming-Dong Bai; Liang-Qun Rong; Lian-Chen Wang; Hai Xu; Rui-Fang Fan; Pei Wang; Xiao-Peng Chen; Liu-Bin Shi; Shu-You Peng


    AIM: To assess the patency of pancreaticoenterostomy and pancreatic exocrine function after three surgical methods. METHODS: A pig model of pancreatic ductal dilation was made by ligating the main pancreatic duct. After 4 wk ligation, a total of 36 piglets were divided randomly into four groups. The piglets in the control group underwent laparotomy only; the others were treated by three anastomoses: (1) end-to-end pancreaticojejunostomy invagination (EEPJ); (2) end-to-side duct-to- mucosa sutured anastomosis (ESPJ); or (3) binding pancreaticojejunostomy (BPJ). Anastomotic patency was assessed after 8 wk by body weight gain, intrapancreatic ductal pressure, pancreatic exocrine function secretin test, pancreatography, and macroscopic and histologic features of the anastomotic site. RESULTS: The EEPJ group had significantly slower weight gain than the ESPJ and BPJ groups on postoperative weeks 6 and 8 (P < 0.05). The animals in both the ESPJ and BPJ groups had a similar body weight gain. Intrapancreatic ductal pressure was similar in ESPJ and BPJ. However, pressure in EEPJ was significantly higher than that in ESPJ and BPJ (P < 0.05). All three functional parameters, the secretory volume, the flow rate of pancreatic juice, and bicarbonate concentration, were significantly higher in ESPJ and BPJ as compared to EEPJ (P < 0.05). However, the three parameters were similar in ESPJ and BPJ. Pancreatography performed after EEPJ revealed dilation and meandering of the main pancreatic duct, and the anastomotic site exhibited a variable degree of occlusion, and even blockage. Pancreatography of ESPJ and BPJ, however, showed normal ductal patency. Histopathology showed that the intestinal mucosa had fused with that of the pancreatic duct, with a gradual and continuous change from one to the other. For EEPJ, the portion of the pancreatic stump protruding into the jejunal lumen was largely replaced by cicatricial fibrous tissue. CONCLUSION: A mucosa-to-mucosa pancreatico

  16. 小切口保留黄韧带手术治疗腰椎间盘突出症的研究%Analysis on treatment of lumbar disc herniation by minimal incision surgery with preserving the ligamentum flavum

    杨永林; 胡泽元; 金霖峰; 高强


    Objective To evaluate the indications and effects of treatment of lumbar disc herniation by minimally incision fenestration laminectomy and lumbar discectomy surgery with preserving the ligamentum flavum.Methods Minimally incision fenestration laminectomy and lumbar discectomy surgery with preserving the ligamentum flavum were performed in 183 cases of lumbar disc herniation.The shallow ligamentum flavum was peeled and the deep ligamentum flavum was preserved.After lumbar discectomy, bring the ligamentum flavum back to its original location.Results 183 cases were followed up for 6 months to 3 years; the excellent rate was 90.2%.The patients were examined with CT and MRI after operation, the result showed that the ligamentum flavum was no deformation and dent, the scar tissue of spinal canal were stopped on ligamentum flavum.Conclusion Treatment of lumbar disc herniation by minimal incision fenestration lamineetomy and lumbar discectomy surgery with preserving the ligamentum flavum can effectively prevent cicatricial adhesion in canales spinalis.%目的 探讨小切口椎板间开窗保留黄韧带的椎间盘髓核摘除术治疗腰椎间盘突出症的疗效及适应证.方法 对183例腰椎间盘突出症患者行小切口椎板间开窗保留黄韧带的腰椎间盘髓核摘除术,剥离浅层黄韧带,保留深层黄韧带.摘除椎间盘髓核后将深层黄韧带恢复到原来的位置.结果 183例随访6个月~3年,优良率达90.2%,术后复查CT或MRI,可见相应黄韧带无变形或塌陷,椎管外瘢痕组织于黄韧带处中止.结论 小切口椎板间开窗保留黄韧带的腰椎间盘髓核摘除术能有效地防止术后椎管内瘢痕粘连.

  17. Estudo comparativo do uso tópico de própolis verde e vermelha na reparação de feridas em ratos

    Lara Lívia Valença Batista


    Full Text Available OBJETIVO: Avaliar a ação cicatrizante de própolis verde e vermelha, correlacionando ao teor de flavonoides MÉTODOS: Foi realizada a quantificação de flavonoides totais dos extratos etanólicos de própolis verde e vermelha para posterior incorporação em pomada base a 20%. Utilizou-se 20 ratos Wistar distribuídos em quatro grupos: solução salina a 0,9% (S, pomada base (B, pomada própolis verde (G, pomada própolis vermelha (R, todos submetidos à confecção de lesões excisivas na região mediana do dorso. Os ratos foram tratados diariamente durante 15 dias. Neste período foram observados peso; temperatura corporal; diâmetro das feridas. Para análise histológica, amostras das feridas foram coletadas. Ao fim do experimento foram realizadas coleta sanguínea e remoção do rim e fígado para análises bioquímica e histológica. RESULTADOS: Os teores de flavonoides totais das própolis verde (4,50 % e vermelha (5,92 % foram elevados (>2 %, mas, embora a segunda apresente teor maior que a primeira, a evolução da própolis verde, macro e histologicamente, foi melhor na reparação das feridas. Não foram observadas nefro ou hepatotoxicidade, resultado corroborado pelos ensaios bioquímicos (TGP e albumina. A própolis influiu na redução de colesterol total, triglicerídeos e glicemia. CONCLUSÃO: Não foi possível correlacionar o teor de flavonoides totais com a ação cicatrizante da própolis. O dado revela a necessidade da elucidação dos flavonoides encontrados em cada classe de própolis para desvendar qual (ou quais flavonoide(s seria(m representativo(s no processo cicatricial.

  18. Biopolímero produzido a partir da cana-de-áçucar para cicatrização cutânea Sugar cane biopolymer in cutaneous healing

    Maria Cristina de Oliveira Cardoso Coelho


    Full Text Available Um polissacarídeo extracelular foi produzido por via microbiológica, através da bactéria ZSP isolada no Laboratório de Microbiologia Industrial da Estação Experimental de Cana-de-Açúcar do Carpina/UFRPE, apresentando excepcional capacidade de processo. Os principais monossacarídeos presentes na fração solúvel foram glicose (87,6%, xilose (8,6%, manose (0,8%, ribose (1,7%, galactose (0,1%, arabinose (0,4% e o ácido glucurônico (0,8%. Devido ao alto índice de traumatismos que acomete os animais domésticos e a busca por alternativas simples, econômicas e capazes de proporcionar condições ideais para cicatrização, foram realizados os testes com o biopolímero produzido a partir da cana-de-açúcar em animais portadores de feridas cutâneas, a fim de avaliar a reepitelização. Observou-se o aumento do tecido de granulação, controle da infecção e diminuição do tempo de cicatrização, permitindo concluir que o biopolímero contribui para o processo cicatricial, podendo ser utilizado em feridas cutâneas.An extracelular polysaccharide was produced through microbiology, using the ZSP bacteria isolated in the Industrial Microbiological Laboratory of the Sugar cane Experimental Station at Carpina/UFRPE, Pernambuco, Brazil, presenting exceptional process capacity. The principal monosaccharides present in the ¹soluble fraction were glucose (87.6%, xylose (8.6%, mannose (0.8%, ribose (1.7%, galactose (0.1%, arabinose (0.4% and the glucuronic acid (0.8%. Due to the high rate of injuries that occur with domestic animals and the search for simple, economical alternatives that would be capable of giving ideal conditions for the healing process, extensive testing was done with the biopolymer produced by sugar cane in animals that had cutaneous wounds, so as to evaluate the reepitelization process. It was observed from the testing results that there was better skin granulation, better infection control, and less healing time, which

  19. Clinical Analysis of 30 Cases of Primary Incision plus HGH Soluble Hemostatic Gauze in Treating Perianal Abscess%一次切开术结合HGH溶解止血纱布治疗肛周脓肿30例



    目的探讨一次切开术结合HGH溶解止血纱布(以下称止血纱布)在治疗肛周脓肿中的疗效。方法自肛缘沿脓肿顶部两侧呈放射状梭形切开脓肿,寻找内口并切开,清理脓腔,止血纱布填塞脓腔,术后抗菌素应用,每日便后清洗换药,重新填塞止血纱布至痊愈。结果30例患者均治疗一次痊愈。结论一次切开术结合止血纱布治疗肛周脓肿具有疼痛轻,创面生长快,并发症少,病程短等特点;在本组病例中患者无明显疼痛、创面渗液少、脓腔生长快、无1例术后出血、无1例瘢痕挛缩、无1例肛门狭窄及失禁;术后随访1年,无1例复发。%Probe into the medical ef ect of single incision with HGH gauze in treating perianal abscess. Methods Start incision from the top of abscess, search for a inside access and cut, clean the abscess, pack HGH gauze, use antibiotic after operation, clean the incision and reuse of antibiotic after daily excretion, repack HGH gauze til completely cured. Results Al 30 patients were completely cured by only single treatment. Conclusion The single incision with HGH gauze in treating perianal abscess has following advantages: less pain, less liquid excretion and quick recovery of operation surface. There is no case showing cicatricial contracture, anus stenosis and incontinence of feces. And there is no case of relapse during postoperative fol ow-up for 1 year.

  20. Autologous serum for ocular surface diseases Soro autólogo para doenças da superfície ocular

    Guilherme Goulart Quinto


    Full Text Available Autologous serum has been used to treat dry eye syndrome for many years. It contains several growth factors, vitamins, fibronectin and other components that have been considered important for corneal and conjunctival integrity. Serum eye drops are usually prepared as an unpreserved blood solution. The serum is by nature well tolerated and its biochemical properties are somewhat similar to natural tears. Autologous serum eye drops have been reported to be effective for the treatment of severe dry eye-related ocular surface disorders (Sjögren's syndrome, and also other entities such as superior limbic keratoconjunctivitis, graft-versus-host disease, Stevens-Johnson syndrome, ocular cicatricial pemphigoid, recurrent or persistent corneal erosions, neurotrophic keratopathy, Mooren's ulcer, aniridic keratopathy, filtering blebs after trabeculectomy, and post-keratorefractive surgery. The purpose of this study is to review the recently published literature on ocular surface diseases treated with human autologous serum eye drops.O soro autólogo tem sido adotado como uma nova abordagem para tratar síndrome do olho seco porque contém vitaminas, alguns fatores de crecimento e fibronectina que são considerados importantes contribuintes para integridade corneana e conjuntival. Colírio de soro autólogo é produzido sem preservativo. O soro é não-alérgico e suas propriedades bioquímicas são similares à lágrima. O soro autólogo tópico tem sido relatado efetivo para o tratamento de olho seco grave relacionado a distúrbios da superfície ocular como na síndrome de Sjögren, ceratoconjuntivite límbica superior, doença do enxerto versus hospedeiro, síndrome de Stevens-Johnson, procedimentos cerato-refrativos, erosão corneana persistente ou recorrente, ceratopatia neurotrófica, úlcera de Mooren, ceratopatia associada à aniridia, e bolhas filtrantes após trabeculectomia. O objetivo do presente estudo é revisar a literatura recentemente

  1. Experimental strabismus surgery using triamcinolone: outcomes and effects on inflammatory response Utilização da triancinolona como agente modulador da resposta inflamatória na cirurgia de estrabismo em coelhos

    Luis Eduardo Morato Rebouças de Carvalho


    Full Text Available PURPOSE: To evaluate the efficiency of triamcinolone (TRI in limiting the postoperative inflammatory response and scarring after strabismus surgery. METHODS: A prospective, two-stage, masked, controlled trial was conducted. In the first stage, the inflammatory response at the extraocular muscle reattachment site was analyzed after superior rectus recession in ten rabbits. In the second stage, TRI (40 mg/ml was applied during surgery to the eyes of 16 rabbits with superior rectus recession. As a control, contralateral eyes were treated with physiological saline. Fifteen days later, exenteration was performed, and the sites of muscle reattachment were processed for histological examinations. The sums of the areas of the granulomas in the extraocular muscle reattachment sites of control and treated eyes were compared. RESULT: There was a preliminary inhibition effect of TRI on the inflammatory response of treated eyes compared with that of control eyes. CONCLUSIONS: In the conditions of conducting this study the introperative use of TRI was effective in controlling the postoperative inflammatory response in rabbit eyes after extraocular muscle surgery.OBJETIVO: Avaliar a eficiência da triancinolona (TRI como agente modulador da resposta inflamatória e cicatricial em coelhos submetidos à cirurgia de estrabismo. MÉTODOS: Foi realizado estudo prospectivo, mascarado, em dois estágios. No primeiro estágio 10 coelhos foram submetidos a retrocesso do músculo reto superior em ambos os olhos, aplicando-se triancinolona (40 mg/ml em um dos olhos e como controle, solução salina nos olhos contralaterais. Quinze dias e trinta dias após, os animais foram exenterados e o material do sítio de reinserção muscular foi submetido à análise qualitativa e quantitativa. No segundo estágio, com incrementação da agressão cirúrgica, 16 coelhos foram submetidos aos mesmos procedimentos com exenteração e análise dos tecidos após 15 dias. RESULTADO


    Ştefania Bejan


    Full Text Available Devenit loc comun în istoria contemporană a lumii, fenomenul crizei economice apare fără preaviz și lasă cicatrici adânci în memoria individuală și colectivă, în proiectele sociale (politice, militare à la long în relațiile interumane…Oricât nu s-ar recunoaște, nimeni nu e vaccinat definitiv împotriva „bacilului crizei”; fiecare om rămâne marcat (o vreme, pentru totdeauna, sensibil, fundamental: unii savurează cu disperarea ultimei zile de existență momente, întâmplări, prietenii, dispute, călătorii, iubiri ,bani, orgolii; alții se luptă cu înverșunare să supraviețuiască, să-și dovedească puterea de a fi răzbit în pofida tuturor opreliștilor; unii se consolează, lipsiți de energia tinereții, a unui nou început, a unui miracol gen „Ce-ar fi, dacă ?”. Profesioniștii comunicării, alături de inși poposiți din eroare în domeniu (ne referim cu precădere la politicienii fără cea mai firavă vocație în acest sens își adaptează (strategic ? intuitiv? discursul destinat spațiului public și oferă o „stagiune” pestriță, tocmai bună de studiat la „Constituție”, „Cultură civică”, „PR în O.N.G.” etc. Accentele descoperite de cercetător țin proaspătă agenda mass-media și dau de lucru cetățenilor cu ceva brumă de preocupare vizând normalitatea societății în care s-au „nimerit”…

  3. A bumblefoot outbreak and fatal septicemia in captive aquatic birds in Brazil Surto de esparavão e septicemia fatal em aves aquáticas cativas no Brasil

    Marcus Vinícius Romero Marques


    Full Text Available A bumblefoot outbreak with different prognosis according to host species was studied in captive aquatic avian species. Six wood ducks (Aix sponsa, three scarlet-ibis (Eudocimus ruber, two black-swans (Cygnus atratus, five white-faced ducks (Dendrocygna viduata and two roseate spoonbills (Platalea ajaja were kept in a common pen with abrasive pavement pond margin, predisposing to podal skin wear. Incoordination and mortality occurred in the two roseate spoonbils and one black swan. Coagulase-positive penicillin-resistant Staphylococcus aureus was isolated from the synovial fluid and from the liver. All birds sharing the pen presented active or cicatricial foot lesions, indicating a possible challenge to the environmental Staphylococci. However, except for the roseate spoonbill and the black swan, which had fatal disease, for all other species the case did not evolve to a clinically debilitating or fatal disease. The different susceptibility to a fatal Staphylococcus aureus coagulase positive infection is discussed.Este trabalho relata um surto de esparavão em aves aquáticas cativas com prognóstico variado conforme a espécie de ave. Foram examinados um marreco carolina (Aix sponsa, três guarás (íbis vermelha (Eudocimus ruber, dois cisnes negros (Cygnus atratus, cinco marrecas piadeiras (Dendrocygna viduata e dois colhereiros (Platalea ajaja mantidos em recinto comunitário, em cativeiro e em criatório. No recinto, a piscina é margeada por borda de cimento abrasivo que possibilita a abrasão da pele podal. Todas as aves apresentaram erosões da pele na face de apoio dos pés, indicando fator predisponente (erosão similar e possível semelhante desafio infeccioso. Entretanto, incoordenação e mortalidade ocorreram em colhereiros e no cisne negro, mas não nas outras espécies. Staphylococcus aureus coagulase positivo e resistente à penicilina foi isolado do líquido sinovial e do fígado das aves recém-mortas e conservadas em geladeira

  4. Diagnostic and interventional radiology in the post-operative period and follow-up of patients after rectal resection with coloanal anastomosis

    Severini, A.; Civelli, E.M.; Uslenghi, E.; Cozzi, G.; Salvetti, M.; Milella, M. [Department of Radiology, National Cancer Institute of Milan, via Venezian 1, I-23100 Milan (Italy); Gallino, G.; Bonfanti, G.; Belli, F.; Leo, E. [Department of Surgery, National Cancer Institute of Milan, via Venezian 1, I-23100 Milan (Italy)


    was cured in 1 week. In 36 cases of cicatricial stenosis, 17 at the coloanal anastomosis and 19 at the pouch, radiological examination always detected the lesion, correctly defining its anatomical characteristics, nature and extension. Of the 19 cases of stenosis treated radiologically, 15 recovered an adequate intestinal calibre for normal evacuation. During follow-up of the 175 patients operated on, 21 cases of recurrence were detected. Radiological examination was requested as the first investigation in only one of these cases, for a patient with subocclusion. Radiological investigations in patients who have undergone coloanal anastomosis are of real diagnostic value in the immediate post-operative period, during closure of the protective colostomy and in the follow-up of symptomatic recanalized patients. (orig.)

  5. Ação da l-arginina na evolução de retalhos cutâneos de ratos sob exposição à nicotina

    Marcus Vinicius Thomé Nora Guimarães


    Full Text Available OBJETIVO: Avaliar se o tratamento com L-arginina influencia a cicatrização de retalhos cutâneos em ratos expostos à nicotina. MÉTODOS: Foram utilizados 40 ratos Wistar pesando 142,4±10,1g separados em quatro grupos: GC- tratamento com solução tampão fosfatos pH 7,4, confecção de retalho cutâneo, observação por 10 dias; GN- exposição à nicotina por quatro semanas, confecção de retalho cutâneo, observação por dez dias; GA- tratamento com solução tampão fosfatos pH 7,4 por quatro semanas, confecção de retalho cutâneo, tratamento com arginina por dez dias; GAN- exposição à nicotina por quatro semanas, confecção de retalho cutâneo, tratamento com arginina por dez dias. Foram avaliadas as áreas de necrose, re-epitelização, reação inflamatória e formação de tecido de granulação, pela coloração HE, a área de deposição total e a diferenciação de colágenos I e III por histometria com a coloração de picrosirius, e, através da marcação imunoistoquímica com anticorpo monoclonal anti-CD34, a densidade vascular cicatricial. RESULTADOS: As porcentagens de áreas de necrose de GN e GNA foram maiores (p0,05 e maiores (p<0,001 do que em GA e em GNA e, nas densidades vasculares, GN e GAN foram menores (p<0,001 do que em GC e em GA. CONCLUSÃO: A exposição à nicotina inibiu os efeitos da arginina, e nos ratos não expostos, induziu melhora na angiogênese e na deposição de colágeno total nos retalhos cutâneos.

  6. Dacryocystorhinostomy ostium: parameters to evaluate and DCR ostium scoring

    Ali MJ


    Full Text Available Mohammad Javed Ali,1 Alkis James Psaltis,2 Peter John Wormald2 1Dacryology Service, L V Prasad Eye Institute, Hyderabad, Telangana, India; 2Department of Surgery–Otorhinolaryngology, Head and Neck Surgery, The University of Adelaide, Adelaide, SA, Australia Aim: This study aims to provide a systematic protocol for the evaluation of a dacryocystorhinostomy (DCR ostium and to propose a scoring system to standardize the assessment.Methods: Retrospective evaluation of 125 consecutive lacrimal ostia post-DCR was performed. Medical records were screened, and photographs and videos were assessed to note the details of various ostial parameters. The major time points in evaluation were 4 weeks, 6 weeks, 3 months, and 6 months post-DCR. The ostia were defined and parameters like shape, size, location, and evolution of ostium were noted. Evaluation parameters were defined for internal common opening (ICO, ostium stents, and ostium granulomas. Ostium cicatrix and synechiae were graded based on their significance. Surgical success rates were computed and ostium characteristics in failed cases were studied.Results: A total of 125 ostia were evaluated on the aforementioned ostium parameters. Because five ostia showed a complete cicatricial closure with no recognizable features, the remaining 120 ostia were studied. The ostium location was anterior to the axilla of middle turbinate in 85.8% (103/120 of the cases. Moreover, 76.6% (92/120 of the ostia were circular to oval in shape, with a shallow base. The ostium size was >8×5 mm in 78.3% (94/120 of the cases. The ICO was found to be located in the central or paracentral basal area in 75.8% (91/120. The anatomical and functional success rates achieved were 96% and 93.6%, respectively. All the five cases with anatomical failures showed a complete cicatrization and the ICO movements were poor in all the three cases of functional failure.Conclusion: The article attempts to standardize the postoperative

  7. Resection And Reconstructive-plastic Surgery In Treatment Of Extended And Multifocal Cicatrical Tracheal Stenosis

    Otabek Eshonkhodjaev


    Full Text Available Background. Main issues of treatment of patients with cicatricial stenosis of trachea (CST are to increase its efficacy and safety, as well as to determine indications and contraindications for circular resection and reconstructive interventions on the trachea in patients with extended lesions of trachea, with stenoses involving subvocal cords part of larynx and well as multifocal narrowing of the trachea which could improve treatment results and quality of life of patients, to gain recovery and reduce disability and mortality.Material and methods. One hundred two patients aged from 11 to 62 years with CST were surgically treated. Stenotic site length in patients varied from 0,3 to 7 cm. Most frequently (64,2%, CST length of more than 2 cm was observed. In critical and decompensated stenosis with diameter of CST up to 5 mm with the threat of asphyxiation by the first stage we used endoscopic laser-and electric destruction of constriction followed by restricted zone bouging. If long-term rehabilitation was necessary and in case of absence of the conditions to the implementation of circular tracheal resection, we used tracheal stents. Circular resection of the trachea was performed in 24 patients. In case of contraindications, 28 patients underwent reconstructive plastic surgery with dissection the stenosis, excision of scar tissue and formation of trachea lumen in T-tube. After removing T-tube plasty of anterior tracheal wall and of soft tissues of the neck defects was performed using local tissues and grafts with microsurgical techniques.Results. Long-term results of treatment evaluated and studied in a period of 6 months to 5 years in 89 (87.3% patients. 13 patients continues to one of the stages of complex treatment. Еhe patients are under dynamic endoscopic control. Good results were achieved in the treatment of 71 patients (79.8%, satisfactory - in 12 (13.4%, unsatisfactory results in 6 (6.7% patients.Conclusions. 1. Patients with CST

  8. Cistoplastia experimental em coelhos (Oryctolagus cuniculus com peritônio bovino conservado em glicerol a 98% Experimental cystoplasty in rabbits (Oryctolagus cuniculus with bovine peritoneum conserved in 98% glycerol

    Tatiana Catelan de Oliveira


    Full Text Available Neste estudo foi implantado um retalho de membrana peritoneal bovina em substituição a um fragmento da face ventral da bexiga de coelhos albinos, raça Nova Zelândia, adultos, machos não-castrados (n=12, com o intuito de avaliar o processo de reparação tecidual no que se refere à biocompatibilidade, à capacidade de reparação tecidual e a possíveis complicações. Aos sete, 14, 30 e 60 dias de pós-operatório, os animais foram eutanasiados, três em cada período, mediante o emprego de tiopental sódico (200mg kg-1, para posterior avaliação macroscópica e análise histopatológica da interface do implante com o tecido nativo. Macroscopicamente, foram observadas, em todos os períodos, aderências de estruturas adjacentes ao local do implante, presença de cálculos e ausência de sinais de rejeição. Sob microscopia de luz, aos sete, 14 e 30 dias de observação, o implante ainda estava presente, havia intensa reação inflamatória mista, neovascularização, fibroplasia e escassas fibras musculares, contudo, o epitélio e a lâmina própria não estavam reconstituídos. Aos 60 dias de avaliação, o implante não estava mais presente e todas as camadas vesicais encontravam-se reparadas. O implante foi biocompatível e forneceu arcabouço para orientação e desenvolvimento das camadas teciduais da bexiga, mediante processos de reparação, restabelecendo a estrutura do órgão.Bovine peritoneum was utilized to replace a section of ventral face of the bladder of New Zealand rabbits, adult and entire males (n=12 with the purpose of evaluating the tissue repair process in regard of its biocompatility, scar formation ability and possible complications. The animals were euthanized at 7, 14, 30 and 60 days post operative, three in each period, by the use of sodium thiopental (200mg kg-1, followed by macroscopic evaluation and histopathological analysis of the implant interface with the native tissue. Macroscopically it was observed

  9. Efeito da suplementação nutricional com glicina e glutamina, por via oral, na cicatrização colônica em coelhos Effect of the nutritional supplementation with glicine and glutamine, oral feeding, in the colonic cicatrization in rabbits

    Juliano Alves Figueiredo


    Full Text Available OBJETIVO: Avaliar o efeito de dois aminoácidos, glicina e glutamina, na epitelização da mucosa colônica, tamanho da área de cicatriz, fibroplasia e resistência tênsil da anastomose intestinal, após colectomia parcial e anastomose término-terminal. MÉTODOS: Quarenta e cinco coelhos adultos, com idade entre 24 e 28 semanas, do sexo masculino, com peso inicial médio de 2.362 g, foram distribuídos aleatoriamente, de forma dupla desconhecida, em cinco grupos, de acordo com o tipo de suplemento. Dois animais morreram em cada grupo por causas diversas. Os grupos de coelhos foram assim distribuídos: Grupo 1 - (n = 9 receberam suplementação alimentar com glutamina durante sete dias antes e cinco dias após a operação; Grupo 2 - (n = 9 receberam suplementação alimentar com glicina durante sete dias antes e cinco dias após a operação; Grupo 3 - (n = 9 receberam suplementação alimentar com glutamina durante cinco dias após a operação; Grupo 4 - (n = 9 receberam suplementação alimentar com glicina durante cinco dias após a operação; Grupo 5 - (n = 9 não receberam suplementação alimentar. A resistência da anastomose foi medida por meio de pressão de ruptura por insuflação de ar intraluminar. RESULTADOS: O Grupo 2 foi o único grupo que apresentou valores de ruptura superiores aos do Grupo 5 (p OBJECTIVE: To evaluate the effect of two amino-acids, glycine and glutamine, in the epithelialization of the colonic mucosa, size of the scar area, fibrous as well as the tensile strength of the intestinal anastomoses, after the removal of 17 cm of the colon and an end-to-end with interrupted suture. METHODS: Forty five male adult rabbits varying between 24 and 28 weeks of age with the starting average weight of 2,362 grams were randomly distributed into five groups, according to their respective type of feeding. Two animals died in each group due to different complications. The rabbits were divided into the following groups

  10. Application of biological dural graft made by meninges from porkers

    Peng Liu; Shengping Huang; Songtao Qi


    sections were observed. White blood cells in venous blood were counted before operation and execution, separately. The obtained data were statistically analyzed.MAIN OUTCOME MEASURES: ① Wound healing and recovery following implantation of dural graft.②The amount of white blood cells in venous blood from rabbits at each time point before operation and before execution. ③ Histological examination results of operative site.RESULTS: Sixteen experimental rabbits were involved in the final analysis. ① The experimental rabbits of each group had no local infection, effusion and abnormal appearance. They had good wound healing and were normal to access to food. ② There were no significant differences in amount of white blood cells in venous blood from experimental rabbits between at each time point after modeling and before operation (P >0.05). ③Pathological observation of operative site: At postoperative 3 days, local acute inflammation repair reaction appeared; At postoperative 2 weeks, chronic inflammatory reaction appeared, endodermis in artificial dural graft formed, and artificial dural graft and host dura mater healed; In postoperative 1 month,wound began to chronically recover; In postoperative 3 months, host blood capillary began to form in artificial dural graft based on chronic repair. In all the control sides, fibroplasia was found, and a few neutrophils were found at postoperative 2 weeks.CONCLUSION: Biological surgical patch has high stability and good histocompatibility. It can provide dural epithelial epithelium, effectively prevent against the conglutination of scalp tissue and brain tissue, and avoid the leakage of cerebrospinal fluid.

  11. Stimulation of mucoperiostal slice epithelization by small power laser after the primary plastic of oroantral communication

    Jovanović Goran


    Full Text Available Introduction The oroantral communication is a pathologic communication between the oral cavity and maxillary sinus. It originates with extraction of the upper lateral teeth. Primary plastics communication, which is more extensive than 5 mm has been unsuccessful in 16%. Small power lasers have positive reaction on wounds healing. The aim of this work was to determine the lasers effects on slice epithelization after the plastics more extensive than 5 mm. Material and methods The experimental research was done on dogs with extraction of the upper second premolars on both sides, and formed oroantral communication having diameter of 10 mm. After the plastics of sinus, the left side slices were exposed to radiation for 7 days, and the opposite slices healed spontaneously. Eight points (8x1J were treated for 100s by GaAlAs laser, power 10 mW and wavelength 670 nm. Biopses of the slices connections were taken on the 14th day to be laboratory treated and examined microscopially. The study included 36 examinees with communication diameter of 10 mm and performed plastics of sinuses. In half of the examinees wounds were exposed to radiation for 7 days, and in other examinees they healed spontaneously. The results were analyzed on the fourteenth day according to the scale: 1 - complete healing, without dehiscention; 2 - incomplete healing, with minimal dehiscention; 3 - communication did not heal, with partial dehiscention; 4 - open communication, with significant dehiscention. Results and discussion The microscopic analysis shows that no wounds exposed to radiation were overcast with mucous membrane which had smooth sub epithelia chronic inflammation and inflammable infiltrate, and strong fibroplasias and granulations. Wounds exposed to radiation had mucous membrane without any signals of inflammation. Laser radiation causes anti-inflammatory reaction, i.e. it provokes reduction of exudation, alteration and proliferation, it blocks cyclo- and lipo

  12. Primary entropion in persian cats Entrópio primário em gatos persas

    José Luiz Laus


    Full Text Available Entropion is defined as the inward rolling of the eyelid margin in which the eyelashes and eyelid hair (frequently the lower lateral lid rub the cornea. Etiologies may be congenital, spastic, or cicatricial. This condition usually causes epiphora, blepharospasm, photophobia, conjunctivitis, purulent discharge, corneal vascularization, pigmentation and ulceration, if not surgically treated. Congenital entropion commonly affects dogs and is frequently hereditary in some breeds, whereas cats are uncommonly affected. A predilection for the Persian breed to present primary entropion has been suggested. The authors report two cases of entropion in Persian cats referred to the Ophthalmology Section of Veterinary College of São Paulo State University - UNESP, Jaboticabal - SP / Brazil. First case: a male Persian cat, 2 years old, with a history of bilateral ocular irritation and purulent discharge for 8 months. Ophthalmic examination revealed epiphora, blepharospasm, photophobia, bilateral entropion affecting the whole length of the lower eyelids, conjunctivitis and purulent discharge. Second case: a male Persian cat, 1 year old, with a history of bilateral ocular irritation and purulent discharge for 3 weeks. At ophthalmic examination the animal was presenting epiphora, blepharospasm, photophobia, bilateral entropion affecting the whole length of the lower lids, conjunctivitis, purulent discharge, corneal vascularization, superficial ulceration and edema. The entropion persisted after topical anaestesia in both cats. Surgical treatment was similar in both cases, based on the modified Holtz-Celsus procedure. Grid keratotomy procedure was also performed in the second case. Both cats had a satisfactory clinic evolution which was confirmed few days after surgery.O entrópio é caracterizado por uma inversão da margem palpebral, na qual os cílios e os pêlos da pálpebra (freqüentemente a porção lateral da pálpebra inferior atritam a córnea. A

  13. Crecimiento maxilar según severidad de hendidura labial, alveolar y palatina unilateral Maxillary growth according to the severity of unilateral cleft lip and palate

    M.C. Navas-Aparicio


    Full Text Available La inhibición del crecimiento y desarrollo resultante del tratamiento quirúrgico en pacientes con labio y paladar hendido es un tema ampliamente discutido en el mundo. De acuerdo a la literatura, la deficiencia de tejidos, probablemente debida al ancho y la posición del segmento alveolar en la hendidura, es una variable a considerar que afecta al crecimiento del maxilar, el cual también puede verse influido por la corrección quirúrgica del labio, la nariz y el paladar, por tejido cicatricial, los diferentes tipos de tratamiento quirúrgico, el tiempo de cirugía, la habilidad del cirujano y la ortopedia prequirúrgica. El propósito de este estudio es determinar la posible asociación entre la severidad de la hendidura y el crecimiento maxilar en niños con labio y paladar hendido unilateral no sindrómico nacidos en el año 2001 y atendidos en el Hospital Nacional de Niños "Dr. Carlos Sáenz Herrera", Caja Costarricense de Seguro Social de San José, Costa Rica. El estudio fue de tipo retrospectivo y descriptivo, basado en datos recopilados de registros médicos, modelo inicial de escayola del maxilar del niño recién nacido, radiografía cefalométrica y modelo actual del maxilar del niño a la edad de 5 años. La muestra de estudio comprendió 13 pacientes. La dimensión transversal del arco fue asimétrica en 12 casos, lo que indica alteración del crecimiento en esta dirección. Existe una correlación positiva estadísticamente significativa entre la longitud del arco y el ángulo SNA, indicando que a mayor longitud de arco, el ángulo SNA es mayor. El valor promedio de ANB tiene una desviación importante superior a 2. No existe relación entre la posición y longitud del arco maxilar (SNA, BaNA y ANS-PNS y la severidad de la hendidura. Esto significa, que el tejido cicatricial, el tipo de tratamiento quirúrgico, el tiempo de cirugía, el cirujano, la habilidad del cirujano y la ortopedia prequirúrgica, no influyen en la direcci

  14. 都江堰友爱学校地震伤员康复状况调查分析%Rehabilitation needs of earthquake victims in Dujiangyan Youai primary school in Sichuan Province

    潘福琼; 黎浩然; 杨玉龙; 田金艳; 赵冠兰; 彭博; 陈启明; 罗尚蔚; 何锦华; 彭元宏


    Objective To investigate the status of dysfunction and rehabilitation needs of earthquake victims in Dujiangyan Youai primary school in Sichuan Province,to provide a guideline for further rehabilitation. Methods Live investigation was performed for 105 patients in the school. Amputation Body Image Scale (ABIS)was used as psychological assessment to investigate the psychological status of 40 amputees. Results Among the 105 victims,there were 44 cases of fracture (41.9%) ,40 cases of amputee ( 39% ),15 cases of crush syndrome ( 14. 2% ) and 6 cases of head injury (5. 7% ). Most of them had different levels of physical dysfunction,including 62 cases of decreased muscle strength ( 59. 1% ) ,32 cases of muscular atrophy ( 30. 2% ) ,49 cases of limited range of motion ( ROM ) ( 46. 7% ) , 70 cases of cicatricial adhesion ( 66. 7% ) , 14 cases of sensory disorder ( 13.3% ) , 32 cases of arthrogryposis ( 30. 5% ) ,23 cases of abnormal gait ( 21.9% ) and 26 cases of limitation in activities of daily living ( ADL ) ( 24. 8% ). Conclusions Sichuan earthquake victims in Youai primary school had different levels of rehabilitation needs. Further rehabilitation is needed and intensive attention should be paid to those wounded with psychological problems.%目的 分析全国首家无障碍学校--四川省都江堰友爱学校地震伤员的伤情、功能障碍、康复现状及需求,为下一步康复医疗提供依据.方法 对105例在校伤员进行现场功能评估,采用截肢身体意象评估量表(ABIS),对40名截肢伤员进行问卷调查.结果 105例伤员中,骨折44例(41.9%)、截肢40例(39%)、挤压伤15例(14.2%)、脑外伤6例(5.7%);大都存在的不同程度、多种功能障碍问题,其中肌力减退62例(59.1%),肌肉萎缩32例(30.2%),关节活动度(ROM)受限49例(46.7%),瘢痕粘连增生70例(66.7%),感觉障碍14例(13.3%),关节挛缩32例(30.5%),步态异常23例(21.9%),生活不能完全自理26例(24.8%).结论 105

  15. Complicações e condutas fisioterapêuticas após cirurgia por câncer de mama: estudo retrospectivo Complications and physical therapeutic treatment after breast cancer surgery: a retrospective study

    Simony Lira do Nascimento


    Full Text Available Após cirurgia por câncer de mama, as mulheres estão sujeitas a desenvolver algumas complicações físicas. Os objetivos deste trabalho foram: investigar o desfecho dessas mulheres, que, durante o primeiro mês pós-operatório, foram submetidas a um programa de reabilitação e identificar ao longo de dois anos as complicações mais frequentes e as condutas fisioterapêuticas mais adotadas. Foi um estudo descritivo, retrospectivo, com dados de 707 prontuários de mulheres operadas por câncer de mama no Hospital da Mulher Professor Doutor José Aristodemo Pinotti da Universidade Estadual de Campinas, entre janeiro de 2006 e dezembro de 2007, atendidas pelo Setor de Fisioterapia. A análise foi feita por meio de médias, desvio padrão e frequências absolutas e relativas. Ao final do programa, 55% das mulheres receberam alta, 17% necessitaram de atendimento adicional e 26% não aderiram a ele. As complicações mais frequentes foram: aderência pericicatricial (26%, restrição da amplitude de movimento (24% e deiscência cicatricial (17%. No primeiro ano após a cirurgia (n=460, foram relatados dor (28,5%, sensação de peso (21,5% e restrição da amplitude de movimento do ombro (16,7%; já no segundo (n=168, houve dor (48,2%, sensação de peso (42,8% e linfedema (23,2%. Concluiu-se que, ao final do programa, a maioria das mulheres recebeu alta. Ao longo dos anos, houve redução da frequência de restrição da amplitude de movimento do ombro com aumento de linfedema. Cuidados com o braço, exercícios domiciliares e autodrenagem foram as condutas mais adotadas.After breast cancer surgery, women may develop some physical complications. Thus, the aims of this study were to investigate the outcome of these women, who participated in a rehabilitation program for one month, as well to identify along two years the most frequent complications and adopted physical therapy conducts. It was a descriptive and retrospective study with 707 medical

  16. Viabilidade do baço de ratos após a ligadura dos vasos esplênicos: efeito do tratamento com oxigênio hiperbárico Viability of the spleen in rats after ligation of the splenic vessels: effects of hyperbaric oxygen therapy

    Danilo Nagib Salomão Paulo


    Full Text Available OBJETIVO: Verificar o efeito da ligadura dos vasos esplênicos principais no baço de ratos e a influência do tratamento com o oxigênio hiperbárico após a ligadura. MÉTODOS: Foram operados 69 ratos Wistar, machos, de 285g a 375 g. Os animais foram divididos aleatoriamente em três grupos: grupo 1: quatro ratos, simulação; grupo 2: 34 ratos, ligadura simultânea da artéria e veia esplênica; grupo 3: 31 ratos, ligadura da artéria e veia esplênica seguida de oxigenioterapia hiperbárica no pós-operatório por 11 dias, sendo mortos no 12º dia. O baço era retirado e incluído em parafina para estudo microscópico. RESULTADOS: O baço era normal em 8,82% e 45,16%, respectivamente, no grupo que sofreu a ligadura vascular sem ou com oxigenioterapia hiperbárica (p= 0,01. O percentual de massa viável do tecido esplênico nos baços que infartaram em relação ao percentual da massa corporal dos animais não diferiu entre os grupos 2 e 3. O aspecto histopatológico mostrou arquitetura preservada na porção não infartada nos dois grupos e neoformação conjuntivo-vascular cicatricial mais acentuada no grupo que recebeu oxigênio hiperbárico. CONCLUSÕES: O tratamento com oxigênio hiperbárico, a partir do pós-operatório imediato, após a ligadura simultânea da artéria e da veia esplênicas, reduziu significativamente a freqüência dos infartos, mas não alterou o percentual de massa viável dos baços, quando o infarto ocorreu, e acelerou o processo de cicatrização, com aumento da proliferação de fibroblastos e da neoformação vascular.OBJECTIVE: To investigate the effects of splenic artery and vein ligation and the influence of hyperbaric oxygen after the double vascular ligation on the viability of spleen tissue. Methods: Sixty nine adult male Wistar rats (285-375g were randomly separated in three groups: group 1, four rats, sham operated, group 2, 34 rats, submitted to simultaneous splenic artery and vein ligation and group

  17. In vivo assessment of intratumoral aspirin injection to treat hepatic tumors

    Rogério Saad-Hossne


    cicatricial aspect at 7 d after treatment. At all time points evaluated, all except one biochemical parameters tested within the reference range (P > 0.05; a significant increase was detected in the alkaline phosphatase level of the control group 3 on D11 (P < 0.05. At 24 h post-treatment, the aspirin-treated groups showed extensive coagulation necrosis accompanied by a remarkable absence of viable tumor foci; at 7 d after treatment, the tumors had completely disappeared in these animals and fibrous necrotic nodules had developed. In contrast, throughout the study course, the tumors of the control groups remained unchanged, showing tumor nodules without necrosis at the time point corresponding to 24 h post-treatment and increased amounts of tumor nodules at the time point corresponding to 7 d post-treatment. Quantitative analysis of the remaining tumor area revealed that the aspirin-treated groups had significantly smaller tumor foci at 24 h post-treatment (8.5% ± 0.7% and at 7 d after treatment (11.0% ± 4.2%, compared to those in the control groups (24 h: 98.5% ± 1.5% and 7 d: 94.0% ± 2.7%; both, P < 0.005. CONCLUSION: Intralesional injection of a 10% aspirin solution causes destruction of VX2 hepatic tumors in rabbits without evidence of relapse at 7 d after treatment administration.

  18. Efeito da mitomicina C tópica na cicatrização de prega vocal em modelo suíno Topical mitomycin C effect on swine vocal folds healing

    Paulo Antônio Monteiro Camargo


    Full Text Available Várias terapias adjuvantes à cirurgia vêm sendo usadas para modular o processo cicatricial nas pregas vocais, entre elas a Mitomicina tópica (MTC. OBJETIVOS: Avaliar os efeitos da MTC no processo de cicatrização de pregas vocais de suínos 30 dias após exérese de fragmento de mucosa com laser de CO2 mediante a mensuração da deposição de colágeno. FORMA DE ESTUDO: Experimental em suínos. MATERIAIS E MÉTODOS: Dois grupos de 6 suínos cada foram avaliados após exérese de fragmento de mucosa de prega vocal a laser de CO2 (grupo controle sem uso e grupo experimento com uso de MTC tópica. Após 30 dias os animais foram submetidos à eutanásia, sendo coletadas amostras das pregas vocais para análise histológica, a fim de quantificar a deposição de colágeno mediante coloração de Picrosirius Red. RESULTADOS: A média da área do colágeno total das pregas vocais do grupo controle foi de 2648,03 µm2, enquanto a média do colágeno total das pregas vocais do grupo experimento foi de 2200,30 µm2 (p = 0,0043. CONCLUSÃO: A MTC usada topicamente após a exérese de fragmento de mucosa da prega vocal a laser de CO2 em suínos, diminui, significativamente, a deposição do colágeno total.several adjuvant therapies to surgery have been used to modulate the healing process of vocal folds, including topic mitomycin (MTC. AIM: to evaluate the effect of topical MTC in the healing process of vocal folds 30 days after the exeresis of a mucosal fragment with CO2 laser in a swine model (control group without mitomycin and study group with topical MTC, with collagen deposition measurement. STUDY TYPE: prospective experimental in swine. METHODS: two groups of 6 swine each were subjected to exeresis of a mucosal fragment of the vocal fold with CO2 laser. Immediately after the procedure MTC was applied topically for three minutes on the study group. Thirty days later the animals were slaughtered and samples of the vocal folds were collected for

  19. Manejo del enoftalmos como secuela de fracturas del complejo cigomático-orbitario con apoyo de estereolitografía Management of enophtalmos as sequelae of zygomatic complex orbital fractures with stereolitography model support

    H. Malagón Hidalgo


    Full Text Available El enoftalmos postraumático se define como la discrepancia entre el volumen orbitario y su contenido; este balance puede verse alterado por múltiples factores, como el secuestro tardío del contenido orbitario por atrapamiento en el sitio de la fractura, herniación del contenido orbitario, necrosis del tejido adiposo orbitario, contracción cicatricial del tejido retrobulbar, pérdida del sistema de suspensión ligamentario del globo ocular y aumento de volumen de la cavidad orbitaria. El presente estudio aborda el problema del enoftalmos postraumático como una deformidad difícil de corregir. Están descritos abordajes cutáneos extensos como el coronal, el subciliar o el intraoral; la técnica descrita por Henry Kawamoto en 1998 describe un procedimiento de menor invasión mediante abordajes de mínima exposición que permiten la refracturación y el alineamiento del cuerpo zigomático, brindando así grandes ventajas sobre las técnicas convencionales. Presentamos el método aplicado en el servicio de Cirugía Reconstructiva del Centro Médico del Instituto de Seguridad Social del Estado de México y Municipios (ISSEMyM para el manejo de pacientes con enoftalmos como secuela de traumatismos del complejo cigomático-malar; para ello empleamos un método por asistencia con modelos estereolitográficos para la planificación quirúrgica del diseño de osteotomías para corregir la deformidad facial y para determinar las dimensiones del defecto a reparar, así como el tipo y cantidad de materiales aloplásticos o autólogos a emplear. Compartimos la experiencia de los casos tratados en nuestro Servicio entre 2006 y 2010.Post-traumatic enophthalmos is defined as the discrepancy between the orbital volume and its content; this balance can be altered by many factors, such as the entrapment or herniation of the orbital content at the fracture site, orbital adipose tissue necrosis, scar contraction of the retrobulbar tissue, loss of the eyeball

  20. Measurement of the flexing force of the fingers by a dynamic splint with a dynamometer Medida da força de flexão dos dedos da mão através de órtese dinâmica com dinamômetro

    Silmara Nicolau Pedro da Silva


    ça no tendão flexor diminui conforme aumenta o grau de amplitude articular. Observou uma relação entre o comprimento do dedo e a magnitude da força exercida no tendão durante a flexão do dedo, sendo que nos dedos mais compridos os tendões são submetidos a forças maiores. Quando comparou a estatura e envergadura com a magnitude da força aplicada no tendão flexor, observou uma relação positiva em todos os graus de flexão estudados, exceto a 30º. O sexo masculino apresentou maior força em todos os graus de amplitude articular. CONCLUSÕES: Conclui que é possível medir a força de flexão transmitida pelos tendões flexores através de uma órtese acoplada a um dinamômetro, que esta força é maior nos indivíduos do sexo masculino, com dedos mais longos, de maior altura e envergadura e que tais dados permitirão o desenvolvimento de futuros trabalhos no campo da reabilitação da mão, auxiliando pacientes portadores de lesões de tendões, retração cicatricial, deformidades e rigidez articular.

  1. Estudo experimental comparando o uso do 2-octil cianoAcrilato ao nylon 4-0 na sutura da pele Experimental study comparing 2-octyl cyanoacrylate to nylon for skin closure

    Ruffo de Freitas-Júnior


    Full Text Available OBJETIVO: Comparar o 2-octil cianoacrilato com o fio de "nylon" no fechamento da pele em ratos. MÉTODOS: Vinte e cinco ratos da linhagem Wistar, foram submetidos à incisão de 3 cm de cada lado do abdome. A síntese foi feita utilizando em um dos lados o "nylon" 4.0, pontos intradérmicos, e do outro o 2-octil cianoacrilato. Após sete dias, o fio foi removido e as incisões, analisadas quanto às complicações. Após 40 dias, o resultado da cicatriz foi avaliado. Os ratos foram sacrificados, as cicatrizes foram ressecadas, fixadas e enviadas ao patologista, sem informação sobre qual o método utilizado. RESULTADOS: Houve dois óbitos durante a anestesia e um tardio. O tempo de operação foi de 136 segundos com a cola e 176 segundos, com o "nylon" (P=0,003. Dentre as 50 operações realizadas, as complicações foram: um hematoma com cada método (P=0,80, quinze deiscências da cola contra 11 do "nylon" (P=0,20, sete cicatrizes de aspecto ruim ou razoável da cola contra quatro do "nylon" (P=0,30, três infecções na cola contra duas (P=0,40. Ao exame patológico, a mediana da largura da cicatriz foi de 1.119 micra com a cola e 1.800 com o "nylon" (P=0,40. A espessura foi de 1.795 contra 1.705 micra (P=0,40. CONCLUSÃO: O 2-octil cianoacrilato apresentou o mesmo aspecto cicatricial, a mesma resistência e as mesmas complicações que a sutura com o "nylon" 4.0, porém permitindo redução no tempo cirúrgico.BACKGROUND: Tissue adhesive 2-octyl cyanoacrylate was developed to have more strength, less inflammatory reaction, and less histotoxicity than its shorter-chain derivates. METHODS: To evaluate the use of 2-octil cyanoacrylate in comparison with "nylon" suture in skin closure of rats. Methods: Twenty-five female Wistar rats were submitted to a 3cm surgical incision in each side of the abdomen. The wound was closed with intradermic suture using "nylon" 4-0 in one side, and 2-octil cyanoacrylate on the other side. After 7 days the thread

  2. Estudo comparativo da cicatrização de enterorrafias em planos aposicional e invaginante no cólon descendente de eqüinos Comparative study between appositional and inverting pattern for closure of descending colon enterotomies in equines

    F.A. Lucas


    Full Text Available O estudo foi conduzido com o objetivo de comparar o processo cicatricial do cólon descendente de eqüinos, quando submetido a duas técnicas de sutura, uma em plano aposicional e outra em dois planos com invaginação. Foram utilizados 15 eqüinos sadios e a intervenção cirúrgica foi realizada com os animais em decúbito lateral direito, sob anestesia geral inalatória. O acesso à cavidade abdominal foi feito pelo flanco esquerdo, e após exteriorização do cólon descendente, realizaram-se duas enterotomias de 5cm de extensão cada, distanciadas 20cm uma da outra. Os animais foram aleatoriamente distribuídos em cinco grupos de três animais cada e sacrificados aos 3, 7, 14, 21 e 35 dias pós-operatório. O exame necroscópico revelou presença de aderências nos dois padrões de sutura. Em nove eqüinos foram observadas aderências no padrão de sutura aposicional (60,0% ± 12,6 e em quatro no padrão com invaginação (26,6 % ± 11,4. Não foi verificada interferência com o trânsito intestinal em nenhum paciente. O exame histopatológico revelou regeneração da camada mucosa a partir do sétimo dia de pós-operatório em ambos os tipos de sutura. O fio de poliglactina 910 empregado nas enterorrafias do cólon descendente se mostrou resistente, de fácil manuseio e com boa capacidade para manter os nós aplicados, causando moderada reação inflamatória do tipo granulomatosa.This trial was carried out in order to compare the healing process of the descending colon in horses, submitted to two distinctive suture techniques - appositional pattern and inverting pattern. Fifteen healthy horses were used and the surgical intervention was accomplished with the animals in right lateral recumbency, under inhalatory anesthesia. The abdominal cavity approach was done by the left flank, and after exteriorization of the descending colon, two enterotomies, 5cm long, and 20cm apart one from each other were done. The horses were randomly distributed

  3. Hernioplastia diafragmática em cão com pericárdio bovino conservado em solução supersaturada de açúcar Diaphragmatic hernioplasty in dogs with bovine pericardium preserved in supersaturated sugar solution

    A. Mazzanti


    Full Text Available Foram utilizados sete cães adultos, três machos e quatro fêmeas, sem raça definida, com pesos entre 10 e 22kg, para avaliação do processo cicatricial do músculo diafragma, na presença do implante de pericárdio bovino conservado em solução supersaturada de açúcar a 300%. Foi criado um defeito na porção muscular do hemidiafragma esquerdo de dimensões 8,0×5,0cm. Após a toracotomia no oitavo espaço intercostal esquerdo, o implante heterógeno foi fixado com fio poliamida nº 3-0 por meio de sutura simples contínua. Decorrido o período pré-estabelecido de pós-operatório, os animais foram submetidos a exames radiográficos simples e contrastado e a estudos macroscópico e histológico. Na avaliação radiográfica, foi verificada presença das silhuetas diafragmática e cardíaca, sem evidências de vísceras abdominais no interior do tórax. Macroscopicamente, notou-se a formação de tecido conjuntivo fibroso semi-transparente que ocluia o defeito diafragmático. O segmento de pericárdio bovino conservado em solução supersaturada de açúcar a 300%, em temperatura ambiente, é substituído por uma fina camada de tecido conjuntivo fibroso e promove a restauração do defeito no músculo diafragma de cão.Seven adult mongrel dogs, three males and four females, weighting between 10 and 22kg were used to evaluate the diaphragmatic muscle healing process. A defect of 8.0×5.5cm was created at the muscular portion of the left hemidiaphragm, through a thoracotomy at the eighth left intercostal space. A segment of bovine pericardium, preserved in 300% sugar solution, was implanted in the diaphragmatic defect. The heterologous implant was sutured with 3.0 polyamide using simple continuous suture. After the observation period, radiologic analysis and macroscopic and histopathologic evaluations were performed. Upon radiographic examination, there was no evidence of interruption of diaphragmatic outline or increased radiopacity at the

  4. Megaesófago en el perro. Revisión bibliográfica y proposición de una nueva clasificación



    Full Text Available El megaesófago puede definirse, desde un punto de vista anatómico, como una dilatación patológica del esófago. Esta dilatación puede ser parcial o total y constituye la patología más frecuentemente encontrada cuando se estudian alteraciones de tránsito esofágico, tanto en humanos como en animales. Clínicamente se caracteriza por un "síndrome de regurgitación" debido a un tránsito insuficiente a nivel del esófago, con alteraciones nutricionales y de crecimiento en los cachorros, presentación frecuente de "neumonía por aspiración". En animales adultos el cuadro clínico más frecuente es adelgazamiento y desnutrición crónica, acompañado también de signos neumónicos. Esta patología reconoce etiologías diversas, pero pueden agruparse según orígenes básicamente en dos grupos : a alteración de tránsito consecutivo a una alteración anatómica de la pared del esófago (estenosis, producida, por ej., por estenosis cicatricial, persistencia de un arco aórtico, tumores, etc. b alteración de tránsito consecutivo a una alteración funcional del sistema neurovegetativo. El presente trabajo, junto con efectuar una revisión bibliográfica del tema en cuestión, propone una nueva clasificación de los diferentes tipos de megaesófago en relación al área afectada y su etiología, lo que a juicio del autor clarifica la comprensión y manejo de la patología.When studying the esophagus in both human beings and animals, the most common pathological finding is a megaesophagus. From a pathological stand point megaesophagus can be defined as an abnormal dilatation which can be partial or total. The clinical characteristic of this disorder is the regurgitation syndrome due to insufficient esophageal transit, accompanied by nutritional and growth deficiencies, in puppies, with weight loss, nutritional deficiences as well as pulmonary complications due to pneumonia by aspiration in adult dogs. This pathology has diverse origins which

  5. The angiogram sign in pulmonary atelectasis studied with helical Computed Tomography. Incidence and value; Il segno dell'angiogramma nelle atelettasie polmonari studiate con Tomografia Computerizzata spirale. Incidenza e validita' semeiologica

    Sandomenico, F.; Catalano, O.; Cusati, B.; Esposito, M.; Siani, A. [Ospedale S. Maria delle Grazie, Pozzuoli, NA (Italy). Servizio di Radiologia


    In this issue it has been investigated the yield of Helical CT in the study of lung vessels, the incidence of the angiogram sign and its actual value. July 1997 to December 1998, were studied 30 cases of pulmonary collapse of different origin. It was found 15 cases of passive collapse (10 from pleural effusion, 2 from diaphragmatic compression, 2 from traumatic pneumothorax, and 1 from isthmic aortic aneurysm), 12 cases of obstructive collapse (9 from bronchogenic carcinoma, 1 from mucoid obstruction, 1 from ilar lymphadenopathy, and 1 from mediastinal cancer), 2 cases of adhesive collapse and 1 case of round atelectasis. All the examinations were performed with the Helical technique during nonionic iodinated contrast agent injection (bolus, 300-350 mg/mL); it was used an automatic injector set at 2-3 mL/s. It was studied the images for the angiogram sign, that is hyperdense bands, usually longer than 2 cm, through the collapsed lung, which correspond to normal pulmonary vascular branches. The angiogram sign was found in 95% of passive (14/15) and in 80% of obstructive (10/12) collapses. The angiogram sign was missing in 1 of 2 adhesive collapses because of vascular distortion from irradiation-related fibrosis. The comet-tail vascular pattern was seen in round atelectasis. It was not found cases of cicatricial collapse. Thanks to its fast acquisition capabilities. Helical CT well depicts peak vascular enhancement, which permitted identification of the angiogram sign in several pulmonary collapse cases. Thus, this sign becomes even less specific, and just one of the signs of pulmonary consolidation. [Italian] Obiettivo di questo articolo e' riportare la validita' della TC spirale nello studio della vascolarizzazione polmonare discutendo sull'incidenza del segno dell'angiogramma nelle atelettasie polmonari e sulla sua effettiva validita' semeiologica. Nel periodo luglio 1997-dicembre 1998 sono stati diagnosticati 30 casi consecutivi di

  6. Alterações inflamatórias provocadas pelo metronidazol em feridas: estudo experimental em ratos Inflammatory alterations provoked by metronidazole in wounds: an experimental study in rats

    Cláudia Paraguaçu Pupo Sampaio


    Full Text Available CONTEXTO: Cerca de 2,7% da população brasileira tem úlceras crônicas nos pés e nas pernas, porcentagem que chega a 10% nos diabéticos e que representa a segunda causa de afastamento do trabalho no Brasil. Isso demonstra a necessidade de se encontrar um produto de baixo custo que favoreça a cicatrização dessas feridas. OBJETIVO: Avaliar os efeitos do metronidazol na cicatrização de feridas por segunda intenção. MÉTODOS: Utilizaram-se 80 ratos machos, em cujos dorsos se produziu uma ferida, distribuindo-se, os animais, em dois grupos de 40. Os ratos do grupo-controle tiveram suas feridas tratadas com solução de NaCl 0,9%, e os pertencentes ao grupo-experimento, com metronidazol 4%. No terceiro, sétimo, 14º e 21º dias, avaliou-se o processo cicatricial por parâmetros macroscópicos, histológicos e imunoistoquímicos. RESULTADOS: A concentração de colágeno foi maior nas cicatrizes dos animais do grupo-experimento em todos os tempos examinados. A concentração de colágeno do tipo I também foi significante no sétimo dia (p = 0,020 e no 21º dia (p = 0,016. O colágeno tipo III mostrou concentração semelhante nos tempos iniciais e apresentou-se com maior concentração no 21º dia (p = 0,005. A angiogênese, avaliada pelo anti-CD34, demonstrou maior número de vasos, no grupo-experimento, com diferença significante no terceiro dia (p CONTEXT: Chronic feet and leg ulcers affect about 2.7% of the Brazilian population, 10% of diabetic patients. The condition represents the second most frequent cause of absence from work in Brazil. This shows the need for a product that promotes healing of these wounds at a low cost. OBJECTIVE: To evaluate the effects of metronidazole on ulcer healing by second intention. METHODS: Eighty male rats divided into two groups of 40 had a wound made on their dorsum. The control group was treated with a 0.9% NaCl solution and the experimental group was treated with 4% metronidazole. On the third

  7. Cytokine profile of rats fed a diet containing shrimp Perfil das citocinas de ratos alimentados com dieta de camarão

    Elizabeth Lage Borges


    Full Text Available OBJECTIVE: Studies have shown that shrimps reduced the tensile strength of scars in rat skin. The aim of the present study was to assess the cytokine profile of rats fed shrimp. METHODS: Group 1 (control received a regular diet and Group 2 (experimental received a diet containing 33% shrimp for nine days. The two diets contained the same amounts of proteins, fats and carbohydrates. Serum cytokine levels were determined by ELISA and a segment of the jejunum was taken to investigate its histological morphology and eosinophil infiltrate. RESULTS: The experimental group had lower serum levels of interleukin-4 (IL-4 (14.4±1.9 versus 18.11±2.6pg/mL; pOBJETIVO: Estudos mostraram que a dieta suplementada com camarão reduziu a resistência cicatricial na pele de ratos. Nesse contexto, o objetivo do presente estudo foi avaliar o perfil das citocinas de ratos que receberam dieta adicionada com camarão. MÉTODOS: Foram comparados um grupo controle e um grupo experimental, que receberam uma dieta enriquecida com camarão (33% durante nove dias. As duas dietas continham quantidades semelhantes de proteínas, lipídeos, e carboidratos. Os níveis séricos de citocinas foram avaliados por ELISA, assim como um segmento de jejuno foi obtido para exame histológico da morfologia e infiltrado de eosinófilos. RESULTADOS: A dieta adicionada com camarão diminuiu os níveis séricos de IL-4 (14,4±1,9 versus 18,11±2,6pg/mL, p<0,05 e IL-10 (5,0±0,98 versus 7,5±1,2pg/mL, p<0,05 e aumentou os níveis séricos de IL-6 (3,2±0,4 versus 17,8±2,3pg/mL, p<0,001 quando comparada com os animais controle. Morfologicamente, a dieta adicionada com camarão causou uma desorganização da arquitetura da mucosa intestinal, juntamente com uma abundância de eosinófilos nas vilosidades jejunais. CONCLUSÃO: Os dados sugerem que a ingestão de dieta adicionada com camarão leva a um aumento significativo da citocina IL-6, juntamente com uma diminuição da citocina

  8. Perfil de proteases de lesões cutâneas experimentais em camundongos tratadas com a lectina isolada das sementes de Canavalia brasiliensis Proteases profile of skin wounds treated with lectin from Canavalia brasiliensis seeds

    Flávio de Oliveira Silva


    Full Text Available O objetivo deste estudo foi determinar o perfil de proteases em lesões cutâneas experimentais tratadas com a lectina isolada das sementes da Canavalia brasiliensis (ConBr livre e conjugada com o seu açúcar específico. Lesões cirúrgicas foram produzidas assepticamente na região dorsal de camundongos (n=120, divididos de acordo com o tratamento empregado: Grupo NaCl (NaCl 150mM, Grupo manose (manose 100mM, Grupo ConBr (ConBr 100µg mL-1 e Grupo ConBr/manose (solução contendo ConBr 100µg mL-1 preparada em manose 100mM. Amostras da área lesada foram coletadas para determinação do perfil de proteases e atividade colagenolítica no 2°, no 7° e no 12° dia de pós-operatório. O perfil das proteínas realizado através de eletroforese SDS-PAGE demonstrou a presença de proteínas com massa molecular de 67kDa em todos os grupos. O Grupo ConBr/manose apresentou a maior atividade colagenolítica no 12° dia de pós-operatório. A lectina isolada das sementes da Canavalia brasiliensis influenciou a expressão de proteases com atividade colagenolítica podendo assim interferir no processo cicatricial das lesões cutâneas em camundongos.The objective of the present study was determining the proteases profile of cutaneous healings treated with free and conjugated lectin of Canavalia brasiliensis (ConBr and their specific sugar. An aseptic wound was produced in the thoracic area of the mice (n=120, divided according to the employed treatment: NaCl Group (150mM NaCl, manose Group (100mM manose, ConBr Group (100µg mL-1 ConBr and ConBr/manose Group (solution containing 100µg mL-1 ConBr prepared in 100mM manose. Samples of the injured area were collected for determination of proteases profile and collagenolytic activity on 2nd, 7th e 12th days after the surgery. Electrophoresis SDS-PAGE demonstrated proteins with molecular mass of 67kDa in all groups. Group IV presented the highest collagenolytic activity on the 12th day post surgery. Con

  9. Condição ocular dos indivíduos facectomizados na região centro-oeste do estado de São Paulo: estudo populacional Cataract-operated individuals at the Center-Western zone of São Paulo state: populational survey

    Olívia Matai


    % of the patients had visual acuity improved with refractive error correction. The main causes of poor vision were refractive error, posterior capsule opacification (19.4%, bullous keratophaty (8.3%, cicatricial chorioretinitis (8.3%, aphakic eyes (8.3%, age-related macular degeneration (5.5%, leukoma (5.5%, glaucoma (5.5%, optic atrophy (5.5%, retinal detachment (2.8%, retinal pigment epithelium atrophy (2.8% and high myopia (2.8%. CONCLUSION: Cataract projects are effective in reducing preventable blindness caused by lens opacity. However, long-term scheduled evaluation of operated patients is necessary avoiding consequent blindness resulting from neglected follow-up.

  10. Atividade antibacteriana e cicatrizante do óleo de buriti Mauritia flexuosa L. Antibacterial and healing activities of buriti oil Mauritia flexuosa L.

    Jael Soares Batista


    Full Text Available O objetivo deste estudo foi avaliar a atividade antibacteriana in vitro e cicatrizante do óleo de buriti (M. flexuosa em feridas realizadas em ratos (Rattus norvegicus albinus. Para a avaliação antibacteriana in vitro, foram utilizados cinco patógenos bacterianos incluindo espécies gram-positivas e espécies gram-negativas mediante o uso do método de difusão em ágar. Para a avaliação da atividade cicatrizante, foram utilizados 40 ratos da linhagem Wistar, divididos em dois grupos: o grupo I, composto por 20 ratos com feridas cutâneas, tratados com aplicação tópica do creme base com 10% de óleo de buriti, e o grupo II, controle, com o mesmo número de animais que receberam a aplicação tópica do creme base. A aplicação do produto foi realizada em feridas padronizadas, circulares de 1cm de diâmetro na região dorsolombar. As avaliações clínica, morfométrica e histopatológica das feridas foram realizadas no 3°, 7°, 14° e 21° dias. Em relação à avaliação da atividade antibacteriana, os resultados mostraram que houve inibição do crescimento bacteriano em quatro dos cinco patógenos testados. Em relação à área da ferida, foi observada redução significativa da área no 14o dia e maior percentual de contração das feridas do grupo tratado em relação ao controle. No décimo quarto dia, as feridas tratadas com o óleo do buriti apresentavam aumento significativo na contagem de fibroblastos e fibras colágenas, além de completo processo de reepitelização, enquanto o grupo controle necessitava de mais tempo para resolução do processo cicatricial.The aim of this study was to evaluate the in vitro antimicrobial activity and wound healing effect of buriti oil (M. flexuosa in rats. To evaluate the in vitro antimicrobial activity, five species of bacteria, including both gram-negative and gram-positive, were tested by the agar diffusion method. To assess the wound healing effect, 40 rats of Wistar lineage were