23 CFR 1200.24 - Program income.

Science.gov (United States)

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Program income. 1200.24 Section 1200.24 Highways... Implementation and Management of the Highway Safety Program § 1200.24 Program income. (a) Inclusions. Program income includes income from fees for services performed, from the use or rental of real or personal...

34 CFR 1200.110 - Self-evaluation.

Science.gov (United States)

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Self-evaluation. 1200.110 Section 1200.110 Education Regulations of the Offices of the Department of Education (Continued) NATIONAL COUNCIL ON DISABILITY... COUNCIL ON DISABILITY § 1200.110 Self-evaluation. (a) The agency shall, by November 28, 1994, evaluate its...

23 CFR 1200.25 - Improvement plan.

Science.gov (United States)

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Improvement plan. 1200.25 Section 1200.25 Highways... Implementation and Management of the Highway Safety Program § 1200.25 Improvement plan. If a review of the Annual... improvement plan. This plan will detail strategies, program activities, and funding targets to meet the...

Further Development of HS Field Theory

Science.gov (United States)

Abdurrahman, Abdulmajeed; Faridani, Jacqueline; Gassem, Mahmoud

2006-04-01

We present a systematic treatment of the HS Field theory of the open bosonic string and discuss its relationship to other full string field theories of the open bosonic string such as Witten's theory and the CVS theory. In the development of the HS field theory we encounter infinite dimensional matrices arising from the change of representation between the two theories, i.e., the HS field theory and the full string field theory. We give a general procedure of how to invert these gigantic matrices. The inversion of these matrices involves the computation of many infinite sums. We give the values of these sums and state their generalizations arising from considering higher order vertices (i.e., more than three strings) in string field theory. Moreover, we give a general procedure, on how to evaluate the generalized sums, that can be extended to many generic sums of similar properties. We also discuss the conformal operator connecting the HS field theory to that of the CVS string field theory.

DFT study on oxidation of HS(CH2) m SH ( m = 1-8) in oxidative desulfurization

Science.gov (United States)

Song, Y. Z.; Song, J. J.; Zhao, T. T.; Chen, C. Y.; He, M.; Du, J.

2016-06-01

Density functional theory was employed for calculation of HS(CH2) m SH ( m = 1-8) and its derivatives at B3LYP method at 6-31++g ( d, p) level. Using eigenvalues of LUMO and HOMO for HS(CH2) m SH, the standard electrode potentials were estimated by a stepwise multiple regression techniques (MLR), and obtained as E° = 1.500 + 7.167 × 10-3 HOMO-0.229 LUMO with high correlation coefficients of 0.973 and F values of 43.973.

42 CFR 493.1200 - Introduction.

Science.gov (United States)

2010-10-01

... 42 Public Health 5 2010-10-01 2010-10-01 false Introduction. 493.1200 Section 493.1200 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... Introduction. (a) Each laboratory that performs nonwaived testing must establish and maintain written policies...

24 CFR 235.1200 - Authority.

Science.gov (United States)

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Authority. 235.1200 Section 235... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES MORTGAGE... § 235.1200 Authority. In accordance with the authority contained in section 235(r) of the National...

Determination of Sesquiterpenes in Wines by HS-SPME Coupled with GC-MS

OpenAIRE

Cincotta, Fabrizio; Verzera, Antonella; Tripodi, Gianluca; Condurso, Concetta

2015-01-01

The sesquiterpene compounds present in red wines were characterized and quantified by Headspace Solid-Phase Microextraction in combination with Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS). Sixteen sesquiterpenes were identified, mainly hydrocarbons but also derived oxygenated compounds. Sesquiterpenes were acyclic, monocyclic, byciclic and tryciclic. Sesquiterpenes were detected in SIM (selected ion monitoring) mode using their characteristics ions. All the sesquiterpenes were identi...

7 CFR 1200.18 - Ex parte communications.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Ex parte communications. 1200.18 Section 1200.18 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... Governing Proceedings To Formulate and Amend an Order § 1200.18 Ex parte communications. (a) At no stage of...

21 CFR 73.1200 - Synthetic iron oxide.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Synthetic iron oxide. 73.1200 Section 73.1200 Food... COLOR ADDITIVES EXEMPT FROM CERTIFICATION Drugs § 73.1200 Synthetic iron oxide. (a) Identity. (1) The color additive synthetic iron oxide consists of any one or any combination of synthetically prepared...

30 CFR 250.1200 - Question index table.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Question index table. 250.1200 Section 250.1200 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR... Security § 250.1200 Question index table. The table in this section lists questions concerning Oil and Gas...

25 CFR 1200.5 - What are the advantages and disadvantages of managing trust funds under the options in § 1200.4?

Science.gov (United States)

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false What are the advantages and disadvantages of managing trust funds under the options in § 1200.4? 1200.5 Section 1200.5 Indians OFFICE OF THE SPECIAL TRUSTEE... General Provisions § 1200.5 What are the advantages and disadvantages of managing trust funds under the...

SN 2011hs

DEFF Research Database (Denmark)

Bufano, F.; Pignata, G.; Bersten, M.

2014-01-01

Observations spanning a large wavelength range, from X-ray to radio, of the Type IIb supernova 2011hs are presented, covering its evolution during the first year after explosion. The optical light curve presents a narrower shape and a fainter luminosity at peak than previously observed for Type I...

Correlation of Progranulin, Granulin, Adiponectin and Vaspin with Metaflammation (hs-CRP in Indonesian Obese Men

Directory of Open Access Journals (Sweden)

Rosalia E Napitupulu

2013-08-01

Full Text Available BACKGROUND: Obesity is closely related to chronic, low grade systemic inflammation (metaflammation and it leads to further metabolic complications such as hypertension, atherosclerosis, and type 2 diabetes due to the adipocytokine imbalance. This study was carried out to assess the correlation between progranulin, granulin, adiponectin and visceral adipose tissue-derived serine protease inhibitor (Vaspin with metaflammation (high sensitivity C-reactive protein (hs-CRP in centrally obese men. METHODS: This study was observational with a cross sectional design involving 60 men aged 30-60 years, consisted of 43 obese men (waist circumference (WC ≥90 cm and 13 non obese men (WC 105 cm. CONCLUSIONS: We found metaflammation (hs-CRP was significantly correlated with Vaspin, but not with progranulin, granulin and adiponectin, in obese men. We suggest the possibility of a dynamic expression of adipokines related to WC that are subjected to adipocytes hypertrophy-hyperplasia phenomenon. KEYWORDS: progranulin, granulin, adiponectin, Vaspin, hs-CRP, metaflammation, central obesity.

34 CFR 1200.170 - Compliance procedures.

Science.gov (United States)

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Compliance procedures. 1200.170 Section 1200.170 Education Regulations of the Offices of the Department of Education (Continued) NATIONAL COUNCIL ON... agency shall notify the Architectural and Transportation Barriers Compliance Board upon receipt of any...

21 CFR 886.1200 - Optokinetic drum.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Optokinetic drum. 886.1200 Section 886.1200 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... optokinetic drum is a drum-like device covered with alternating white and dark stripes or pictures that can be...

10 CFR 2.1200 - Scope of subpart L.

Science.gov (United States)

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Scope of subpart L. 2.1200 Section 2.1200 Energy NUCLEAR... Hearing Procedures for NRC Adjudications § 2.1200 Scope of subpart L. The provisions of this subpart... and request the application of Subpart L procedures, and proceedings for the direct or indirect...

Fasting plasma chenodeoxycholic acid and cholic acid concentrations are inversely correlated with insulin sensitivity in adults

Directory of Open Access Journals (Sweden)

Laville Martine

2011-07-01

Full Text Available Abstract Background Accumulating data suggest a novel role for bile acids (BAs in modulating metabolic homeostasis. BA treatment has been shown to improve glucose tolerance and to increase energy expenditure in mice. Here, we investigated the relationship between fasting plasma BAs concentrations and metabolic parameters in humans. Findings Fasting plasma glucose, insulin and lipid profile were measured in 14 healthy volunteers, 20 patients with type 2 diabetes (T2D, and 22 non-diabetic abdominally obese subjects. Insulin sensitivity was also assessed by the determination of the glucose infusion rate (GIR during a hyperinsulinemic-euglycemic clamp in a subgroup of patients (9 healthy and 16 T2D subjects. Energy expenditure was measured by indirect calorimetry. Plasma cholic acid (CA, chenodeoxycholic acid (CDCA and deoxycholic acid (DCA concentrations were analyzed by gas chromatograph-mass spectrometry. In univariable analysis, a positive association was found between HOMA-IR and plasma CDCA (β = 0.09, p = 0.001, CA (β = 0.03, p = 0.09 and DCA concentrations (β = 0.07, p Conclusions Both plasma CDCA, CA and DCA concentrations were negatively associated with insulin sensitivity in a wide range of subjects.

Does cooperativity influence the lifetime of the photo-induced HS state?

International Nuclear Information System (INIS)

Letard, Jean-Francois; Costa, Jose Sanchez; Marcen, Silvia; Carbonera, Chiara; Desplanches, Cedric; Kobayashi, Atsushi; Daro, Nathalie; Guionneau, Philippe; Ader, Jean-Pierre

2005-01-01

We have first recalled the T(LIESST) procedure which consists to determine the temperature above which the photo-magnetic effect is erased. In addition we have selected to series of iron(II) spin crossover complexes, the [Fe(PM-L) 2 (NCS) 2 ] and [Fe(bpp) 2 ]X 2 ·nH 2 O families, to analyse the influence of the cooperativity on the stability of the photo-induced HS state. Some of these complexes exhibit gradual thermal spin crossover behaviours while some others undergo an abrupt thermal transition, with and without hysteresis. Interestingly, whatever the cooperativity effect on the thermal spin crossover transition, the lifetime of the metastable state of all these derivates remains governed by the T(LIESST) = T 0 - 0.31 T 1/2 relation. Finally, we have investigated the magnetic and the photomagnetic properties of a [Fe(bpp) 2 ]-Nafion film. Once more the role of the cooperativity on the stability of the photoinduced HS state appears minor. Conversely, the influence of the nature and the geometry of the inner coordination sphere appears preponderant

[hsCRP protein in children and adolescents with diabetes type 1].

Science.gov (United States)

Głowińska-Olszewska, Barbara; Urban, Mirosława; Peczyńska, Jadwiga; Koput, Alicja

2007-01-01

HsCRP protein is known as a novel marker of low grade inflammatory state, which characterises an atherosclerotic process in its early stages. Contrary to a large amount of data on inflammatory markers in diabetes type 2 and metabolic syndrome in adults, little is known so far about the inflammatory process in diabetes type 1, especially in children. The aim of the study was to estimate the level of hsCRP protein in children and adolescents with diabetes type 1 depending on coexisting additional risk factors for atherosclerosis and microvascular complications. 127 children and adolescents with diabetes duration 6.7+/-3.3 years, aged 14.9+/-3.1, were studied. The control group consisted of 52 healthy children aged 14.9+/-2.8 years, matched acc. to gender. HsCRP level was assessed with use of immunoturbidymetric, latex augmented method (Tina-quant CRP (Latex) HS, Roche). HsCRP in the whole study group was nearly significantly higher compared to control group: 0.17+/-0.2 vs. 0.078+/-0.1 mg/dl, p=0.072. In diabetic hypertensive children (n=38) we found significantly higher levels of hsCRP compared to controls (0.27+/-0.3 vs. 0.07 mg/dl, p=0.008) and compared to diabetic normotensive children (0.13+/-0.22 mg/dl; p=0.024). Diabetic obese patients (n=23) had significantly higer hsCRP compared to controls (0.24+/-0.3 vs. 0.07+/-0.1 mg/dl, p=0.04). In 14 studied diabetic children we found coexisting hypertension and obesity, and we found further increase in hsCRP level - 0.28+/-0.3 mg/dl. In diabetic children with microangiopathy hsCRP level was 0.22+/-0.2 mg/dl, and it was insignificantly higher compared to controls and to diabetic children without complications. Correlation analysis showed interrelations between hsCRP and systolic blood pressure (r=0.2; p=0.04) and HbA1c (r=0.25; p=0.015). In stepwise regression analysis hsCRP was related to systolic blood pressure, HbA1c and the triglycerides level (R=0.37; p=0.003). In children and adolescents with diabetes type 1 we

Tissue-Specific Methylation of Long Interspersed Nucleotide Element-1 of Homo Sapiens (L1Hs) During Human Embryogenesis and Roles in Neural Tube Defects.

Science.gov (United States)

Wang, L; Chang, S; Guan, J; Shangguan, S; Lu, X; Wang, Z; Wu, L; Zou, J; Zhao, H; Bao, Y; Qiu, Z; Niu, B; Zhang, T

2015-01-01

Epigenetic regulation of long interspersed nucleotide element-1 (LINE-1) retrotransposition events plays crucial roles during early development. Previously we showed that LINE-1 hypomethylation in neuronal tissues is associated with pathogenesis of neural tube defect (NTD). Herein, we further evaluated LINE-1 Homo sapiens (L1Hs) methylation in tissues derived from three germ layers of stillborn NTD fetuses, to define patterns of tissue specific methylation and site-specific hypomethylation at CpG sites within an L1Hs promoter region. Stable, tissue-specific L1Hs methylation patterns throughout three germ layer lineages of the fetus, placenta, and maternal peripheral blood were observed. Samples from maternal peripheral blood exhibited the highest level of L1Hs methylation (64.95%) and that from placenta showed the lowest (26.82%). Between samples from NTDs and controls, decrease in L1Hs methylation was only significant in NTD-affected brain tissue at 7.35%, especially in females (8.98%). L1Hs hypomethylation in NTDs was also associated with a significant increase in expression level of an L1Hs-encoded transcript in females (r = -0.846, p = 0.004). This could be due to genomic DNA instability and alternation in chromatins accessibility resulted from abnormal L1Hs hypomethylation, as showed in this study with HCT-15 cells treated with methylation inhibitor 5-Aza.

7 CFR 1200.1 - Words in the singular form.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Words in the singular form. 1200.1 Section 1200.1 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... Governing Proceedings To Formulate and Amend an Order § 1200.1 Words in the singular form. Words in this...

Intralesional triamcinolone for flares of hidradenitis suppurativa (HS)

DEFF Research Database (Denmark)

Riis, Peter Theut; Boer, Jurr; Prens, Errol P

2016-01-01

(triamcinolone acetonide 10 mg/mL) in the management of acute flares in HS. METHODS: This was a prospective case series evaluating the effect of intralesional corticosteroids for alleviation of acute flares in HS. Physician- and patient-reported outcomes were noted. RESULTS: Significant reductions in physician......-assessed erythema (median score from 2-1, P edema (median score from 2-1, P

HS06 Benchmark for an ARM Server

Science.gov (United States)

Kluth, Stefan

2014-06-01

We benchmarked an ARM cortex-A9 based server system with a four-core CPU running at 1.1 GHz. The system used Ubuntu 12.04 as operating system and the HEPSPEC 2006 (HS06) benchmarking suite was compiled natively with gcc-4.4 on the system. The benchmark was run for various settings of the relevant gcc compiler options. We did not find significant influence from the compiler options on the benchmark result. The final HS06 benchmark result is 10.4.

HS06 benchmark for an ARM server

International Nuclear Information System (INIS)

Kluth, Stefan

2014-01-01

We benchmarked an ARM cortex-A9 based server system with a four-core CPU running at 1.1 GHz. The system used Ubuntu 12.04 as operating system and the HEPSPEC 2006 (HS06) benchmarking suite was compiled natively with gcc-4.4 on the system. The benchmark was run for various settings of the relevant gcc compiler options. We did not find significant influence from the compiler options on the benchmark result. The final HS06 benchmark result is 10.4.

Genetic diversity and striatal gene networks: focus on the heterogeneous stock-collaborative cross (HS-CC mouse

Directory of Open Access Journals (Sweden)

Belknap John

2010-10-01

Full Text Available Abstract Background The current study focused on the extent genetic diversity within a species (Mus musculus affects gene co-expression network structure. To examine this issue, we have created a new mouse resource, a heterogeneous stock (HS formed from the same eight inbred strains that have been used to create the collaborative cross (CC. The eight inbred strains capture > 90% of the genetic diversity available within the species. For contrast with the HS-CC, a C57BL/6J (B6 × DBA/2J (D2 F2 intercross and the HS4, derived from crossing the B6, D2, BALB/cJ and LP/J strains, were used. Brain (striatum gene expression data were obtained using the Illumina Mouse WG 6.1 array, and the data sets were interrogated using a weighted gene co-expression network analysis (WGCNA. Results Genes reliably detected as expressed were similar in all three data sets as was the variability of expression. As measured by the WGCNA, the modular structure of the transcriptome networks was also preserved both on the basis of module assignment and from the perspective of the topological overlap maps. Details of the HS-CC gene modules are provided; essentially identical results were obtained for the HS4 and F2 modules. Gene ontology annotation of the modules revealed a significant overrepresentation in some modules for neuronal processes, e.g., central nervous system development. Integration with known protein-protein interactions data indicated significant enrichment among co-expressed genes. We also noted significant overlap with markers of central nervous system cell types (neurons, oligodendrocytes and astrocytes. Using the Allen Brain Atlas, we found evidence of spatial co-localization within the striatum for several modules. Finally, for some modules it was possible to detect an enrichment of transcription binding sites. The binding site for Wt1, which is associated with neurodegeneration, was the most significantly overrepresented. Conclusions Despite the marked

An Evaluation of the Performance Diagnostic Checklist-Human Services (PDC-HS) Across Domains.

Science.gov (United States)

Wilder, David A; Lipschultz, Joshua; Gehrman, Chana

2018-06-01

The Performance Diagnostic Checklist-Human Services (PDC-HS) is an informant-based tool designed to assess the environmental variables that contribute to poor employee performance in human service settings. Although the PDC-HS has been shown to effectively identify variables contributing to problematic performance, interventions based on only two of the four PDC-HS domains have been evaluated to date. In addition, the extent to which PDC-HS-indicated interventions are more effective than nonindicated interventions for two domains remains unclear. In the current study, we administered the PDC-HS to supervisors to assess the variables contributing to infrequent teaching of verbal operants and use of a timer by therapists at a center-based autism treatment program. Each of the four PDC-HS domains was identified as contributing to poor performance for at least one therapist. We then evaluated PDC-HS-indicated interventions for each domain. In addition, to assess the predictive validity of the tool, we evaluated various nonindicated interventions prior to implementing a PDC-HS-indicated intervention for two of the four domains. Results suggest that the PDC-HS-indicated interventions were effective across all four domains and were more effective than the nonindicated interventions for the two domains for which they were evaluated. Results are discussed in terms of the utility of the PDC-HS to identify appropriate interventions to manage therapist performance in human service settings.

Relationship of HS CRP and Sacroiliac Joint Inflammation in Undifferentiated Spondyloarthritis.

Science.gov (United States)

Liu, Te-Jung; Chang, Cheng-Chiang; Chen, Liang-Cheng; Chu, Heng-Yi; Hsu, Chun-Sheng; Chang, Shin-Tsu

2018-01-01

Elevation of serum high sensitivity C-reactive protein (hs-CRP) level has been demonstrated as a risk factor for varying diseases, as well as a biomarker for predicting recovery after operation of lumber disc herniation. Our objective was to investigate the relationship between serum hs-CRP and sacroiliac (SI) joint inflammation in patients with undifferentiated spondyloarthritis (uSpA). In this retrospective study, we enrolled patients with uSpA who underwent hs-CRP testing between January 2007 and September 2013. Serum hs-CRP was analyzed at our central laboratory. All enrolled patients underwent skeletal scintigraphic scan with quantitative sacroiliac measurement. A total of 29 patients were enrolled with mean age 32.27 years and female:male ratio of 6:23. Pearson's correlation coefficient showed a significant difference between hs-CRP in serum and SI/S ratio in uSpA, particularly the middle part of the sacroiliac joint, either right side or left side. The significantly high concentration of serum hs-CRP might indicate a systemic inflammatory response to flare-up of the SI joint and might be an indicator of SI inflammation in uSpA.

Relationship of HS CRP and Sacroiliac Joint Inflammation in Undifferentiated Spondyloarthritis

Science.gov (United States)

Liu, Te-Jung; Chang, Cheng-Chiang; Chen, Liang-Cheng; Chu, Heng-Yi; Hsu, Chun-Sheng; Chang, Shin-Tsu

2018-01-01

Abstract Objective Elevation of serum high sensitivity C-reactive protein (hs-CRP) level has been demonstrated as a risk factor for varying diseases, as well as a biomarker for predicting recovery after operation of lumber disc herniation. Our objective was to investigate the relationship between serum hs-CRP and sacroiliac (SI) joint inflammation in patients with undifferentiated spondyloarthritis (uSpA). Methods In this retrospective study, we enrolled patients with uSpA who underwent hs-CRP testing between January 2007 and September 2013. Serum hs-CRP was analyzed at our central laboratory. All enrolled patients underwent skeletal scintigraphic scan with quantitative sacroiliac measurement. Results A total of 29 patients were enrolled with mean age 32.27 years and female:male ratio of 6:23. Pearson’s correlation coefficient showed a significant difference between hs-CRP in serum and SI/S ratio in uSpA, particularly the middle part of the sacroiliac joint, either right side or left side. The significantly high concentration of serum hs-CRP might indicate a systemic inflammatory response to flare-up of the SI joint and might be an indicator of SI inflammation in uSpA. PMID:29785410

Novel chenodeoxycholic acid-sodium alginate matrix in the microencapsulation of the potential antidiabetic drug, probucol. An in vitro study.

Science.gov (United States)

Mooranian, Armin; Negrulj, Rebecca; Mikov, Momir; Golocorbin-Kon, Svetlana; Arfuso, Frank; Al-Salami, Hani

2015-01-01

We previously designed, developed and characterized a novel microencapsulated formulation as a platform for the targeted delivery of Probucol (PB) in an animal model of Type 2 Diabetes. The objective of this study is to optimize this platform by incorporating Chenodeoxycholic acid (CDCA), a bile acid with good permeation-enhancing properties, and examine its effect in vitro. Using sodium alginate (SA), we prepared PB-SA (control) and PB-CDCA-SA (test) microcapsules. CDCA resulted in better structural and surface characteristics, uniform morphology, and stable chemical and thermal profiles, while size and rheological parameters remained unchanged. PB-CDCA-SA microcapsules showed good excipients' compatibilities, as evidenced by differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy studies. CDCA reduced microcapsule swelling at pH 7.8 at both 37 °C and 25 °C and improved PB-release. CDCA improved the characteristics and release properties of PB-microcapsules and may have potential in the targeted oral delivery of PB.

46 CFR 154.1200 - Mechanical ventilation system: General.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Mechanical ventilation system: General. 154.1200 Section... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo Area: Mechanical Ventilation System § 154.1200 Mechanical ventilation system: General. (a...

21 CFR 892.1200 - Emission computed tomography system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Emission computed tomography system. 892.1200... (CONTINUED) MEDICAL DEVICES RADIOLOGY DEVICES Diagnostic Devices § 892.1200 Emission computed tomography system. (a) Identification. An emission computed tomography system is a device intended to detect the...

Normal human serum (HS) prevents oxidant-induced lysis of cultured endothelial cells (ECs)

International Nuclear Information System (INIS)

Callahan, K.S.; Harlan, J.M.

1986-01-01

Most studies demonstrating oxidant lysis of cultured ECs are performed in serum-free media or media containing low concentrations of bovine serum. The authors found that HS protects human and bovine ECs from lysis caused by reagent H 2 O 2 or glucose/glucose oxidase (GO)-generated H 2 O 2 . EC injury was assessed by 51 Cr release, cell detachment, or trypan blue dye exclusion. Protective HS activity was dose-dependent with concentrations greater than or equal to 25% preventing lethal injury. Cytotoxicity at 24 hrs, induced by 20 mU/ml GO, was 90.1 +/- 5.2% without HS vs 1.7 +/- 4.6% with 25% HS present (20 exp). Similar protection was observed with heparinized plasma. Of note, comparable concentrations of bovine serum were devoid of protective activity. Addition of fatty acid-free albumin to the media was also without protective effect. Preliminary characterization showed HS activity was stable to 60 0 C for 30 min, non-dialyzable at 25,000 MW cutoff, and retained in delipidated serum. The HS protection was not merely due to scavenging of exogenous H 2 O 2 as A23187-induced EC lysis was also prevented by HS. Protective activity was not reproduced by purified cerruloplasmin or transferrin. In conclusion, unidentified factor(s) present in HS protect cultured ECs from oxidant-induced lysis. Since endothelium is normally exposed to 100% plasma, the authors suggest that in vitro studies of oxidant-mediated injury be performed in the presence of HS. Factor(s) in HS may play an important role in modulating oxidant-induced vascular injury in vivo

25 CFR 1200.34 - Can a tribe withdraw redeposited funds?

Science.gov (United States)

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false Can a tribe withdraw redeposited funds? 1200.34 Section 1200.34 Indians OFFICE OF THE SPECIAL TRUSTEE FOR AMERICAN INDIANS, DEPARTMENT OF THE INTERIOR AMERICAN INDIAN TRUST FUND MANAGEMENT REFORM ACT Returning Tribal Funds to Trust § 1200.34 Can a tribe withdraw...

The correlation between hs C-reactive protein and left ventricular mass in obese women

Directory of Open Access Journals (Sweden)

Idrus Alwi

2006-06-01

Full Text Available Plasma C-reactive protein (CRP concentrations are increased in obese individuals. In this study, we examined the correlation between hsCRP and left ventricular mass (LV mass. Fourty five healthy obese women and fourty five healthy non obese women as the controls group were studied by echocardiography and hsCRP. There was no significant correlation between hsCRP and left ventricular mass in obese women (r = 0.29, p 0.06. There was a significant correlation between hs CRP and body mass index (r = 0.46, p 0,002, and also hsCRP and visceral fat (r= 0.33, p 0.03. (Med J Indones 2006; 15:100-4 Keywords: hs C-reactive protein, LV mass, obese women

Functional annotation of the genome unravels probiotic potential of Bacillus coagulans HS243.

Science.gov (United States)

Kapse, N G; Engineer, A S; Gowdaman, V; Wagh, S; Dhakephalkar, P K

2018-05-30

Spore forming Bacillus species are widely used as probiotics for human dietary supplements and in animal feeds. However, information on genetic basis of their probiotic action is obscure. Therefore, the present investigation was undertaken to elucidate probiotic traits of B. coagulans HS243 through its genome analysis. Genome mining revealed the presence of an arsenal of marker genes attributed to genuine probiotic traits. In silico analysis of HS243 genome revealed the presence of multi subunit ATPases, ADI pathway genes, chologlycine hydrolase, adhesion proteins for surviving and colonizing harsh gastric transit. HS243 genome harbored vitamin and essential amino acid biosynthetic genes, suggesting the use of HS243 as a nutrient supplement. Bacteriocin producing genes highlighted the disease preventing potential of HS243. Thus, this work established that HS243 possessed the genetic repertoire required for surviving harsh gastric transit and conferring health benefits to the host which were further validated by wet lab evidences. Copyright © 2018. Published by Elsevier Inc.

Vegetable and Fruit Intakes Are Associated with hs-CRP Levels in Pre-Pubertal Girls

Directory of Open Access Journals (Sweden)

Pilar Navarro

2017-03-01

Full Text Available The influence of diet on inflammation in children remains unclear. We aimed to analyze the influence of diet on high-sensitivity C-reactive protein (hs-CRP levels in a pre-pubertal population free of other influences that may affect hs-CRP levels. We determined hs-CRP levels in 571 six- to eight-year-old children using an hs-CRP ELISA kit. Information on food and nutrient intake was obtained through a food-frequency questionnaire. Overall dietary quality was assessed using the Healthy Eating Index (HEI. We found that girls in the highest tertile of hs-CRP levels had a higher intake of saturated fatty acid, and lower intakes of fiber and vitamin E and a lower HEI score when compared to those in tertiles 1 and 2. We also observed a significant decrease in fruit and vegetable intakes by hs-CRP tertile. Factor analysis showed that a dietary pattern that was loaded most strongly with vegetable, fruit, fiber and vitamin A and E intakes correlated negatively (−0.132, p < 0.05 with hs-CRP. No such association was found in boys. In conclusion, our data show that girls with a poorer quality diet show higher hs-CRP levels already at a pre-pubertal age.

RoHS Compliance - Is the Global Electronics Industry Ready?

OpenAIRE

Head, Marieke; Hróarsson, Hallur

2006-01-01

RoHS is an EU directive that was proposed along with the WEEE Directive in 2002 as a part of a plan to promote extended producer responsibility within the electronics industry. Together, these two directives seek to make electrical and electronic equipment easier to manage both in terms of environmental impacts and recycling. The RoHS Directive seeks to remove lead, cadmium, mercury, hexavalent chromium and two brominated flame retardants from all consumer electrical equipment. This paper dea...

23 CFR 1200.30 - Expiration of the right to incur costs.

Science.gov (United States)

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Expiration of the right to incur costs. 1200.30 Section... HIGHWAY SAFETY PROGRAMS Closeout § 1200.30 Expiration of the right to incur costs. Unless extended in accordance with the provisions of § 1200.31 of this part, the right to incur costs under Section 402 expires...

21 CFR 524.1200b - Kanamycin ophthalmic aqueous solution.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Kanamycin ophthalmic aqueous solution. 524.1200b... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS OPHTHALMIC AND TOPICAL DOSAGE FORM NEW ANIMAL DRUGS § 524.1200b Kanamycin ophthalmic aqueous solution. (a) Specifications. The drug, which is in an aqueous...

7 CFR 1200.50 - Words in the singular form.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Words in the singular form. 1200.50 Section 1200.50 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING....50 Words in the singular form. Words in this subpart in the singular form shall be deemed to import...

25 CFR 39.1200 - Definitions.

Science.gov (United States)

2010-04-01

... School Operation and Maintenance Fund § 39.1200 Definitions. Contract school operation and maintenance costs for fiscal year 1979 means the sum of costs for custodial salaries and fringe benefits, related supplies and equipment and equipment repair, insurance, and school operation utilities costs, where such...

Developing competitive marketing and sales strategy for HS-Eden

OpenAIRE

Dragusha, Cajup

2016-01-01

Start-ups are faced with variety of challenges and uncertainty therefore comprehensive marketing and sales strategy must be in place to make sure that limited resources are spend wisely in order to minimize uncertainty and pave a path that would lead to successful business. HS Eden is a new-start-up venture created in Lappeenranta University of Technology with an ambition to develop AMB systems for commercial use. The aim of this study was to build a marketing and sales strategy to help HS-Ed...

Solutol HS 15, nontoxic polyoxyethylene esters of 12-hydroxystearic acid, reverses multidrug resistance.

Science.gov (United States)

Coon, J S; Knudson, W; Clodfelter, K; Lu, B; Weinstein, R S

1991-02-01

A recently developed non-ionic surfactant called Solutol HS 15 (poly-oxyethylene esters of 12-hydroxystearic acid), with low toxicity in vivo, was shown to reverse completely the multidrug resistance of KB 8-5 and KB 8-5-11 human epidermoid carcinoma cells in vitro but did not potentiate drug toxicity in drug-sensitive KB 3-1 cells. At a concentration of 10% of its own IC50 (mean concentration of drug that causes 50% inhibition of cell growth compared to controls), Solutol HS 15 produced a 35-, 28-, and 42-fold reduction in the resistance of KB 8-5-11 cells to colchicine, vinblastine, and doxorubicin, respectively. Solutol HS 15 was relatively much more potent than the prototypic reversing agent, verapamil, for reversing colchicine resistance, compared to the ability of each agent to reverse colchicine resistance, compared to the ability of each agent to reverse vinblastine resistance. Like verapamil, Solutol HS 15 promoted a 50-fold accumulation of rhodamine 123 in KB 8-5-11 cells, as measured by flow cytometry. Also, Solutol HS 15 and verapamil reduced the efflux of rhodamine 123 from KB 8-5-11 cells previously loaded with rhodamine 123 to a similar low rate. Solutol HS 15 did not affect the transport of alanine or glucose into KB 8-5-11 cells, indicating that its effect upon membrane active transport is not entirely nonspecific. Considering their different structure and different relative potency for reversing colchicine resistance, Solutol HS 15 and verapamil probably reverse multidrug resistance by different mechanisms. Solutol HS 15 merits consideration as a potential therapeutic agent because of its effectiveness for reversing multidrug resistance in vitro and its low toxicity in vivo.

From Viking to Crusader. Scandinavia and Europe 800-1200

DEFF Research Database (Denmark)

1st edtion in Danish: Viking og Hvidekrist. Norden og Europa 800-1200, 1992; 2nd edition 1993. Swedish edition: Från Vikingar till Korsfarare, 1992. German edition: Wikinger Waräger Normannen. Die Skandinavier und Europa 800-1200, 1992. French edition with J.-P. Mohen & F.-X. Dillmann: Les Vikings...

Association of HS6ST3 gene polymorphisms with obesity and ...

Indian Academy of Sciences (India)

The heparan sulfate 6-O-sulfotransferase 3 (HS6ST3) gene is involved in heparan sulphate and heparin metabolism, and has been reported to be associated with diabetic retinopathy in type 2 diabetes. We hypothesized that HS6ST3 gene polymorphisms might play an important role in obesity and related phenotypes (such ...

The Antifungal Plant Defensin HsAFP1 Is a Phosphatidic Acid-Interacting Peptide Inducing Membrane Permeabilization

Directory of Open Access Journals (Sweden)

Tanne L. Cools

2017-11-01

Full Text Available HsAFP1, a plant defensin isolated from coral bells (Heuchera sanguinea, is characterized by broad-spectrum antifungal activity. Previous studies indicated that HsAFP1 binds to specific fungal membrane components, which had hitherto not been identified, and induces mitochondrial dysfunction and cell membrane permeabilization. In this study, we show that HsAFP1 reversibly interacts with the membrane phospholipid phosphatidic acid (PA, which is a precursor for the biosynthesis of other phospholipids, and to a lesser extent with various phosphatidyl inositol phosphates (PtdInsP’s. Moreover, via reverse ELISA assays we identified two basic amino acids in HsAFP1, namely histidine at position 32 and arginine at position 52, as well as the phosphate group in PA as important features enabling this interaction. Using a HsAFP1 variant, lacking both amino acids (HsAFP1[H32A][R52A], we showed that, as compared to the native peptide, the ability of this variant to bind to PA and PtdInsP’s is reduced (≥74% and the antifungal activity of the variant is reduced (≥2-fold, highlighting the link between PA/PtdInsP binding and antifungal activity. Using fluorescently labelled HsAFP1 in confocal microscopy and flow cytometry assays, we showed that HsAFP1 accumulates at the cell surface of yeast cells with intact membranes, most notably at the buds and septa. The resulting HsAFP1-induced membrane permeabilization is likely to occur after HsAFP1’s internalization. These data provide novel mechanistic insights in the mode of action of the HsAFP1 plant defensin.

Poly(lactic-co-glycolic) Acid/Solutol HS15-Based Nanoparticles for Docetaxel Delivery.

Science.gov (United States)

Cho, Hyun-Jong; Park, Ju-Hwan; Kim, Dae-Duk; Yoon, In-Soo

2016-02-01

Docetaxel (DCT) is one of anti-mitotic chemotherapeutic agents and has been used for the treatment of gastric cancer as well as head and neck cancer, breast cancer and prostate cancer. Poly(lactic- co-glycolic) acid (PLGA) is one of representative biocompatible and biodegradable polymers, and polyoxyl 15 hydroxystearate (Solutol HS15) is a nonionic solubilizer and emulsifying agent. In this investigation, PLGA/Solutol HS15-based nanoparticles (NPs) for DCT delivery were fabricated by a modified emulsification-solvent evaporation method. PLGA/Solutol HS15/DCT NPs with about 169 nm of mean diameter, narrow size distribution, negative zeta potential, and spherical morphology were prepared. The results of solid-state studies revealed the successful dispersion of DCT in PLGA matrix and its amorphization during the preparation process of NPs. According to the result of in vitro release test, emulsifying property of Solutol HS15 seemed to contribute to the enhanced drug release from NPs at physiological pH. All these findings imply that developed PLGA/Solutol HS15-based NP can be a promising local anticancer drug delivery system for cancer therapy.

7 CFR 1200.51 - Definitions.

Science.gov (United States)

2010-01-01

... Proceedings on Petitions to Modify or To Be Exempted from Research, Promotion and Information Programs § 1200... includes the judge's: (1) Findings of fact and conclusions with respect to all material issues of fact, law or discretion, as well as the reasons or basis thereof; (2) Order; and (3) Rulings on findings...

23 CFR 1200.20 - General.

Science.gov (United States)

2010-04-01

... cost principles govern the implementation and management of State highway safety programs carried out under 23 U.S.C. 402. Cost principles include those referenced in 49 CFR 18.22 and those set forth in... Implementation and Management of the Highway Safety Program § 1200.20 General. Except as otherwise provided in...

Humic derivatives as promising hormone-like materials

Science.gov (United States)

Koroleva, R. P.; Khudaibergenova, E. M.; Kydralieva, K. A.; Jorobekova, Sh. J.

2009-04-01

The aim of this research is to prepare novel bio-inoculants derived from coal humic substances (HS) using bio-solubilization technique. This approach can be considered to some extent as model for supply plants with available nutrients throw the mineralisation of organic matter in soils by bacteria and fungi. Screening for the stable and active microorganisms' strains possessing ability to degrade humic substances was performed. The following subjects were examined using different isolation methods: natural microbial population from city soil, wood rot of Ulmis Pamila and biohumus of vermiculture of Eisenia foetida. Approaches for monitoring the humics-solubilizing fungi growth under liquid surface conditions in the presence of HS, proper conditions of bio-solubilization technique were elaborated. Coal humic acids (HA) from oxidized brown coal (Kyrgyz deposits) were isolated and added to a Czapek nutrient broth which was used either in full strength or without nitrogen source. The individual flasks were inoculated with natural microbial populations of corresponding cultivated soil, biohumus and wood rot samples for 12 months. Evaluation of phyto-hormonal activity of the produced HS and their derivatives in respect to higher plants with auxine and gibberellic tests was performed. To characterize structure of the biopreparations obtained, an experimental approach was undertaken that implies application of different complementary techniques for the structural analysis of biopreparations. As those were used: elemental and functional analysis, FTIR and 1H, 13C NMR spectroscopy and size-exclusion chromatography. According to the elemental composition of HS recovered from microbial cultures, a decrease in carbon and a significant increase of nitrogen in HS reisolated from the full strength broth inoculated with wood-decay microorganisms has been found. If biohumus microorganisms were used as inoculum, only minor changes were detected in the elemental composition of HS. A

Effect of the administration time of HS6101 on hematopoietic recovery in ICR mice injured by cyclophosphamide

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Shuang XING

2015-06-01

Full Text Available Objective　To explore the effect of the administration time of HS6101 on hematopoietic recovery in ICR mice injured by cyclophosphamide (CTX. Methods　One hundred and three male ICR mice were divided into 4 groups: CTX control, HS6101 prevention, HS6101 treatment, and HS6101 prevention+treatment groups. CTX was intraperitoneally injected into the ICR mice at a dose of 100mg/(kg.d for three consecutive days to establish a chemotherapeutics-injured model. HS6101 at a dose of 27μg/mouse in 0.2ml was subcutaneously injected into the mice 1h before the first administration of CTX in HS6101-prevention group, 1h after the last administration of CTX in HS6101 treatment group, and both at 1h before the first administration and 1h after the last administration of CTX in HS6101 prevention + treatment group. Physiological saline was subcutaneously injected into the mice in CTX control group (0.2ml/mouse. 10μl peripheral blood was collected from the caudal vein for WBC, neutrophil lymphocyte, RBC and platelet counts on day -1, 3, 5, 7, 9, 11, 13, 15, 17 with the MEK-7222K cell analyzer, and the cell count was compared between HS6101 treatment mice and CTX control mice. Another 30 male ICR mice were used for bone marrow colony forming unit (CFU assay and bone marrow histopathological examination, and they were assigned into normal control, CTX control, HS6101 prevention, HS6101treatment and HS6101 prevention + treatment groups (each n=6. On the day 4 and day 9 after CTX injection, mice were sacrificed and bone marrow cells were collected from the left femur for mononuclear cell (MNC isolation. 1×104 MNCs were planted in 1.0ml mouse CFU culture medium M3434 and cultured in incubator with the temperature of 37℃, and 5% CO2 for 7 days. After that, granulocyte macrophage-colony-forming unit (GM-CFU, megakaryocyte colony forming unit (MK-CFU, mixture-colony-forming unit (Mix-CFU, burst-forming unit-erythroid (BFU-E and colony-forming unit-erythroid (CFU

Serum hsCRP: A Novel Marker for Prediction of Cerebrovascular Accidents (Stroke).

Science.gov (United States)

Patgiri, Dibyaratna; Pathak, Mauchumi Saikia; Sharma, Pradeep; Kutum, Tridip; Mattack, Nirmali

2014-12-01

Strokes are caused by disruption of the blood supply to the brain. This may result from either blockage or rupture of a blood vessel. Yearly 15 million people worldwide suffer a stroke. India ranks second worldwide in terms of deaths from stroke. The incidence of stroke increases with age affecting the economically productive middle aged population. Hypertension and male sex are other risk factors for stroke. C-Reactive Protein (CRP) is an acute phase protein whose concentration rises in blood following inflammation. Formerly, assays for CRP detected its rise only after significant inflammation. However, recently developed high sensitivity assays (hsCRP) enable the measurement of CRP in individuals who are apparently healthy. Several studies indicate that hsCRP is elevated in individuals who are at risk of developing Coronary Artery Disease or Cerebrovascular events, the elevation may be found years before the first detection of vascular problems. In the absence of other biochemical markers, the present study aimed to evaluate the predictive and diagnostic role of hsCRP in stroke. The study consisted of 50 patients of acute stroke admitted in Gauhati Medical College and Hospital. The control population consisted of two groups - 50 age and sex matched controls with hypertension (Hypertensive control group) and 50 age and sex matched controls with no obvious disease constituted the Normal control group. hsCRP levels were measured in all the groups and compared statistically. hsCRP is an acute phase reactant whose concentration rises in stroke as well as in those at risk. The rise may be identified even before the appearance of risk factors. Hence, hsCRP may be useful as a predictive and diagnostic marker in stroke.

Evaluation of Usefulness of hs-CRP and Ferritin Assays in Patients with Nasal Polyps

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Robert Partyka

2014-01-01

Full Text Available Background. Chronic nature of the nasal polyps, tendency to recurrence, and lack of satisfying treatment need the diagnostic’s parameters which show early inflammatory state as ferritin and hs-CRP. The Aim of Study. Assessment of hs-CRP and ferritin blood levels in nasal polyps patients in evaluation of treatment efficacy. Methods. All 38 patients were divided into 2 groups. Group I included 19 patients with anti-inflammatory therapy 2 weeks after surgery. Group II included 19 patients without anti-inflammatory therapy 2 weeks after surgery. The levels of hs-CRP and ferritin have been assessed before and 2 and 6 weeks after surgical treatment. Results. Research showed statistically significant difference of ferritin’s concentration between examined groups 6 weeks after surgery (P<0.05 and statistically significant difference of hs-CRP concentration 2 and 6 weeks after surgery (P<0.05. Conclusion. (1 The analysis of serum ferritin and hs-CRP concentrations can be useful in early postoperative detection of inflammatory state in patients with nasal polyps and for the effectiveness of therapy. (2 Lack of correlation between mean ferritin and hs-CRP serum levels, at each diagnostic and monitoring stage, shows that they are independent and cannot be determined interchangeably.

Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10.

Science.gov (United States)

Wang, Zhixin; Wang, Yunpeng; Zheng, Li; Yang, Xiaona; Liu, Hongxia; Guo, Jianhua

2014-11-07

Bacillus licheniformis HS10 is a good biocontrol agent against Pseudoperonospora cubensis which caused cucumber downy disease. To identify and characterize the antifungal proteins produced by B.licheniformis HS10, the proteins from HS10 were isolated by using 30-60% ammonium sulfate precipitation, and purified with column chromatography on DEAE Sepharose Fast Flow, RESOURCE Q and Sephadex G-75. And the SDS-PAGE and MALDI-TOF/TOF-MS analysis results demonstrated that the antifungal protein was a monomer with molecular weight of about 55 kDa, identified as carboxypeptidase. Our experiments also showed that the antifungal protein from B. licheniformis HS10 had significantly inhibition on eight different kinds of plant pathogenic fungi, and it was stable with good biological activity at as high as 100°C for 30 min and in pH value ranged from 6 to 10. The biological activity was negatively affected by protease K and 10mM metal cations except Ca(2+). Copyright © 2014 Elsevier Inc. All rights reserved.

47 CFR 64.1200 - Delivery restrictions.

Science.gov (United States)

2010-10-01

... Advertising § 64.1200 Delivery restrictions. (a) No person or entity may: (1) Initiate any telephone call... telephone service, specialized mobile radio service, or other radio common carrier service, or any service... expect them to be included given the identification of the caller and the product being advertised. (6...

30 CFR 77.1200 - Mine map.

Science.gov (United States)

2010-07-01

... SAFETY STANDARDS, SURFACE COAL MINES AND SURFACE WORK AREAS OF UNDERGROUND COAL MINES Maps § 77.1200 Mine...) The location of railroad tracks and public highways leading to the mine, and mine buildings of a permanent nature with identifying names shown; (k) Underground mine workings underlying and within 1,000...

DHS HS-STEM Final Report.

Energy Technology Data Exchange (ETDEWEB)

Russell, Anna Christine [Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

2014-09-01

Throughout my HS-STEM internship, I worked on two different projects with a systems analysis group at Sandia National Laboratories in Livermore, California (SNLCA). The first, and primary, project entailed building a conceptual model of health surveillance detection of a bioterror attack. The second project was much smaller in scope and looked at cost tradeoffs between volumetric and surface decontamination after the release of anthrax in a city. Both projects helped me to understand the challenges of planning for a bioterror attack and the importance of preparedness in the public health sector.

Association between prepulse inhibition of the startle response and latent inhibition of two-way avoidance acquisition: A study with heterogeneous NIH-HS rats.

Science.gov (United States)

Sánchez-González, Ana; Esnal, Aitor; Río-Álamos, Cristóbal; Oliveras, Ignasi; Cañete, Toni; Blázquez, Gloria; Tobeña, Adolf; Fernández-Teruel, Alberto

2016-03-01

This study presents the first evaluation of the associations between responses in two paradigms related to schizophrenia in the genetically heterogeneous NIH-HS rat stock. NIH-HS rats are a stock of genetically heterogeneous animals that have been derived from eight different inbred strains. A rotational breeding schedule has been followed for more than eighty generations, leading to a high level of genetic recombination that makes the NIH-HS rats a unique tool for studying the genetic basis of (biological, behavioral, disease-related) complex traits. Previous work has dealt with the characterization of coping styles, cognitive and anxiety/fear-related profiles of NIH-HS rats. In the present study we have completed their characterization in two behavioral models, prepulse inhibition (PPI) and latent inhibition (LI) of the two-way active avoidance response, that appear to be related to schizophrenia or to schizophrenia-relevant symptoms. We have found that these rats display PPI for each of the four prepulse intensities tested, allowing their stratification in high, medium and low PPI subgroups. When testing these three subgroups for LI of two-way active avoidance acquisition it has been observed that the LowPPI and MediumPPI subgroups present impaired LI, which, along with the fact that the HighPPI group presents significant LI, allows us to hypothesize that responses in these two paradigms are somehow related and that selection of NIH-HS rats for Low vs HighPPI could make a promising animal model for the study of clusters of schizophrenia-relevant symptoms and their underlying neurobiological mechanisms. Copyright © 2015 Elsevier Inc. All rights reserved.

High-normal levels of hs-CRP predict the development of non-alcoholic fatty liver in healthy men.

Directory of Open Access Journals (Sweden)

Jieun Lee

Full Text Available We performed a follow-up study to address whether high sensitivity C-reactive protein (hs-CRP levels within the normal range can predict the development of non-alcoholic fatty liver disease (NAFLD in healthy male subjects. Among15347 male workers between 30 and 59 years old who received annual health check-ups in 2002, a NAFLD-free cohort of 4,138 was followed through December 2009. Alcohol consumption was assessed with a questionnaire. At each visit, abdominal ultrasonography was performed to identify fatty liver disease. The COX proportional hazard model was used to evaluate the relationship between hs-CRP and incident NAFLD. During the follow-up period, 28.8% (1191 of 4138 of participants developed NAFLD. The hazard ratios of NAFLD were increased by hs-CRP categories within the normal range in the non-adjusted model and age-adjusted model. After adjusting for age, exercise, smoking, BMI, systolic BP, triglyceride, and fasting glucose, these incidences were only increased between the lowest and the highest hs-CRP categories. The risk for NAFLD increased as the hs-CRP level increased (p< 0.001. As the hs-CRP level increased within the healthy cohort, the risk of developing NAFLD increased. This trend remained true even if the hs-CRP level remained within the normal range. hs-CRP can be used as a predictor of NAFLD, as well as other obesity-associated diseases. Therefore, individuals with higher hs-CRP levels (even within the normal range may require appropriate follow-up and management to prevent NAFLD development.

Automatic magnetic susceptibility measurements between 4 K and 1200 K; Mesure automatique des susceptibilites magnetiques de 4 K a 1200 K

Energy Technology Data Exchange (ETDEWEB)

Raphael, G [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

1969-07-01

We give a detailed description of a Faraday magnetic susceptibility balance which operates from 4 K to 1200 K. Some preliminary results on platinum and tantalum illustrate the precision and the sensitivity of the measurements. The apparatus has been designed for measurements on the plutonium compounds which present severe health hazards. (author) [French] Nous decrivons en detail un appareil permettant la mesure des susceptibilites magnetiques de 4 K a 1200 K par la methode de FARADAY. Quelques resultats preliminaires sur le platine et le titane montrent la precision et la sensibilite des mesures, L'appareil a ete adapte aux mesures sur les composes particulierement dangereux du plutonium. (auteur)

PERFORMANCE EVALUATION OF OR1200 PROCESSOR WITH EVOLUTIONARY PARALLEL HPRC USING GEP

Directory of Open Access Journals (Sweden)

R. Maheswari

2012-04-01

Full Text Available In this fast computing era, most of the embedded system requires more computing power to complete the complex function/ task at the lesser amount of time. One way to achieve this is by boosting up the processor performance which allows processor core to run faster. This paper presents a novel technique of increasing the performance by parallel HPRC (High Performance Reconfigurable Computing in the CPU/DSP (Digital Signal Processor unit of OR1200 (Open Reduced Instruction Set Computer (RISC 1200 using Gene Expression Programming (GEP an evolutionary programming model. OR1200 is a soft-core RISC processor of the Intellectual Property cores that can efficiently run any modern operating system. In the manufacturing process of OR1200 a parallel HPRC is placed internally in the Integer Execution Pipeline unit of the CPU/DSP core to increase the performance. The GEP Parallel HPRC is activated /deactivated by triggering the signals i HPRC_Gene_Start ii HPRC_Gene_End. A Verilog HDL(Hardware Description language functional code for Gene Expression Programming parallel HPRC is developed and synthesised using XILINX ISE in the former part of the work and a CoreMark processor core benchmark is used to test the performance of the OR1200 soft core in the later part of the work. The result of the implementation ensures the overall speed-up increased to 20.59% by GEP based parallel HPRC in the execution unit of OR1200.

HSV-1 interaction to 3-O-sulfated heparan sulfate in mouse-derived DRG explant and profiles of inflammatory markers during virus infection.

Science.gov (United States)

Sharthiya, Harsh; Seng, Chanmoly; Van Kuppevelt, T H; Tiwari, Vaibhav; Fornaro, Michele

2017-06-01

The molecular mechanism of herpes simplex virus (HSV) entry and the associated inflammatory response in the nervous system remain poorly understood. Using mouse-derived ex vivo dorsal root ganglia (DRG) explant model and single cell neurons (SCNs), in this study, we provided a visual evidence for the expression of heparan sulfate (HS) and 3-O-sulfated heparan sulfate (3-OS HS) followed by their interactions with HSV-1 glycoprotein B (gB) and glycoprotein D (gD) during cell entry. Upon heparanase treatment of DRG-derived SCN, a significant inhibition of HSV-1 entry was observed suggesting the involvement of HS role during viral entry. Finally, a cytokine array profile generated during HSV-1 infection in DRG explant indicated an enhanced expression of chemokines (LIX, TIMP-2, and M-CSF)-known regulators of HS. Taken together, these results highlight the significance of HS during HSV-1 entry in DRG explant. Further investigation is needed to understand which isoforms of 3-O-sulfotransferase (3-OST)-generated HS contributed during HSV-1 infection and associated cell damage.

Research on infrared radiation characteristics of Pyromark1200 high-temperature coating

Science.gov (United States)

Song, Xuyao; Huan, Kewei; Dong, Wei; Wang, Jinghui; Zang, Yanzhe; Shi, Xiaoguang

2014-11-01

Pyromark 1200 (Tempil Co, USA), which is a type of high-temperature high-emissivity coating, is silicon-based with good thermal radiation performance. Its stably working condition is at the temperature range 589~922 K thus a wide range of applications in industrial, scientific research, aviation, aerospace and other fields. Infrared emissivity is one of the most important factors in infrared radiation characteristics. Data on infrared spectral emissivity of Pyromark 1200 is in shortage, as well as the reports on its infrared radiation characteristics affected by its spray painting process, microstructure and thermal process. The results of this research show that: (1) The coating film critical thickness on the metal base is 10μm according to comparison among different types of spray painting process, coating film thickness, microstructure, which would influence the infrared radiation characteristics of Pyromark 1200 coating. The infrared spectral emissivity will attenuate when the coating film thickness is lower or much higher than that. (2) Through measurements, the normal infrared radiation characteristics is analyzed within the range at the temperature range 573~873 K under normal atmospheric conditions, and the total infrared spectral emissivity of Pyromark 1200 coating is higher than 0.93 in the 3~14 μm wavelength range. (3) The result of 72-hour aging test at the temperature 673 K which studied the effect of thermal processes on the infrared radiation characteristics of the coating shows that the infrared spectral emissivity variation range is approximately 0.01 indicating that Pyromark 1200 coating is with good stability. Compared with Nextel Velvet Coating (N-V-C) which is widely used in optics field, Pyromark 1200 high-temperature coating has a higher applicable temperature and is more suitable for spraying on the material surface which is in long-term operation under high temperature work conditions and requires high infrared spectral emissivity.

23 CFR 1200.31 - Extension of the right to incur costs.

Science.gov (United States)

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Extension of the right to incur costs. 1200.31 Section... HIGHWAY SAFETY PROGRAMS Closeout § 1200.31 Extension of the right to incur costs. Upon written request by the State, specifying the reasons therefor, the Approving Official may extend the right to incur costs...

Ig synthesis and class switching do not require the presence of the hs4 enhancer in the 3' IgH regulatory region.

Science.gov (United States)

Vincent-Fabert, Christelle; Truffinet, Véronique; Fiancette, Remi; Cogné, Nadine; Cogné, Michel; Denizot, Yves

2009-06-01

Several studies have reported that regulatory elements located 3' of the IgH locus (namely hs3a, hs1,2, hs3b, and hs4) might play a role during class switch recombination (CSR) and Ig synthesis. While individual deletion of hs3a or hs1,2 had no effect, pairwise deletion of hs3b (an inverted copy of hs3a) and hs4 markedly affected CSR and Ig expression. Among these two elements, hs4 was tentatively presented with the master role due to its unique status within the 3' regulatory region: distal position outside repeated regions, early activation in pre-B cells, strong activity throughout B cell ontogeny. To clarify its role, we generated mice with a clean deletion of the hs4 after replacement with a floxed neo(R) cassette. Surprisingly, and as for previous deletion of hs3a or hs1,2, deletion of hs4 did not affect either in vivo CSR or the secretion level of any Ig isotype. In vitro CSR and Ig secretion in response to LPS and cytokines was not affected either. The only noticeable effects of the hs4 deletion were a decrease in the number of B splenocytes and a decreased membrane IgM expression. In conclusion, while dispensable for CSR and Ig transcription in plasma cells, hs4 mostly appears to contribute to Ig transcription in resting B lymphocytes.

Efficacy of Magnesium Trihydrate of Ursodeoxycholic Acid and Chenodeoxycholic Acid for Gallstone Dissolution: A Prospective Multicenter Trial.

Science.gov (United States)

Hyun, Jong Jin; Lee, Hong Sik; Kim, Chang Duck; Dong, Seok Ho; Lee, Seung-Ok; Ryu, Ji Kon; Lee, Don Haeng; Jeong, Seok; Kim, Tae Nyeun; Lee, Jin; Koh, Dong Hee; Park, Eun Taek; Lee, In-Seok; Yoo, Byung Moo; Kim, Jin Hong

2015-07-01

Cholecystectomy is necessary for the treatment of symptomatic or complicated gallbladder (GB) stones, but oral litholysis with bile acids is an attractive alternative therapeutic option for asymptomatic or mildly symptomatic patients. This study was conducted to evaluate the efficacy of magnesium trihydrate of ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) on gallstone dissolution and to investigate improvements in gallstone-related symptoms. A prospective, multicenter, phase 4 clinical study to determine the efficacy of orally administered magnesium trihydrate of UDCA and CDCA was performed from January 2011 to June 2013. The inclusion criteria were GB stone diameter ≤15 mm, GB ejection fraction ≥50%, radiolucency on plain X-ray, and asymptomatic/mildly symptomatic patients. The patients were prescribed one capsule of magnesium trihydrate of UDCA and CDCA at breakfast and two capsules at bedtime for 6 months. The dissolution rate, response rate, and change in symptom score were evaluated. A total of 237 subjects were enrolled, and 195 subjects completed the treatment. The dissolution rate was 45.1% and the response rate was 47.2% (92/195) after 6 months of administration of magnesium trihydrate of UDCA and CDCA. Only the stone diameter was significantly associated with the response rate. Both the symptom score and the number of patients with symptoms significantly decreased regardless of stone dissolution. Adverse events necessitating discontinuation of the drug, surgery, or endoscopic management occurred in 2.5% (6/237) of patients. Magnesium trihydrate of UDCA and CDCA is a well-tolerated bile acid that showed similar efficacy for gallstone dissolution and improvement of gallstone-related symptoms as that shown in previous studies.

Prediction of HS Soderberg plant PAH emissions from a laboratory evaluation of a pitch

Energy Technology Data Exchange (ETDEWEB)

Castonguay, L.; Mirtchi, A. A.; Proulx, A. L.; Savard, G.; Simard, E.; Steward, N.; Tremblay, C. [Alcan International Ltd., Arvida Research and Development Centre, Jonquiere, PQ (Canada)

1998-12-31

The presence of certain polycyclic aromatic hydrocarbons (PAHs) in coal tar pitch has been identified as a possible limit to the long-term viability of horizontal stud (HS) Soderberg technology, a technology of importance in the aluminum industry. This paper presents the results of a comparative study of pitch PAH content and HS Soderberg cell emissions. Laboratory results are compared with plant emissions for two regular and low PAH pitches with the same softening points. The results indicate the existence of a correlation between pitch PAH content and cell emission, which is valid for regular tar pitches, low tar pitches, as well as for hybrid pitches. These findings make it possible to predict the quantity and distribution of HS Soderberg cell PAH emissions from the analysis of PAHs in the pitch. The results also justify the conclusion that the emission of genotoxic compounds from pitch in the HS Soderberg technology can be decreased by using a pitch with low PAH content. 4 refs., 5 tabs., 5 figs.

A3-Coupling catalyzed by robust Au nanoparticles covalently bonded to HS-functionalized cellulose nanocrystalline films

Directory of Open Access Journals (Sweden)

Jian-Lin Huang

2013-07-01

Full Text Available We decorated HS-functionalized cellulose nanocrystallite (CNC films with monodisperse Au nanoparticles (AuNPs to form a novel nanocomposite catalyst AuNPs@HS-CNC. The uniform, fine AuNPs were made by the reduction of HAuCl4 solution with thiol (HS- group-functionalized CNC films. The AuNPs@HS-CNC nanocomposites were examined by X-ray photoelectron spectroscopy (XPS, TEM, ATR-IR and solid-state NMR. Characterizations suggested that the size of the AuNPs was about 2–3 nm and they were evenly distributed onto the surface of CNC films. Furthermore, the unique nanocomposite Au@HS-CNC catalyst displayed high catalytic efficiency in promoting three-component coupling of an aldehyde, an alkyne, and an amine (A3-coupling either in water or without solvent. Most importantly, the catalyst could be used repetitively more than 11 times without significant deactivation. Our strategy also promotes the use of naturally renewable cellulose to prepare reusable nanocomposite catalysts for organic synthesis.

Fatty acid methyl esters and Solutol HS 15 confer neuroprotection after focal and global cerebral ischemia.

Science.gov (United States)

Lin, Hung Wen; Saul, Isabel; Gresia, Victoria L; Neumann, Jake T; Dave, Kunjan R; Perez-Pinzon, Miguel A

2014-02-01

We previously showed that palmitic acid methyl ester (PAME) and stearic acid methyl ester (SAME) are simultaneously released from the sympathetic ganglion and PAME possesses potent vasodilatory properties which may be important in cerebral ischemia. Since PAME is a potent vasodilator simultaneously released with SAME, our hypothesis was that PAME/SAME confers neuroprotection in rat models of focal/global cerebral ischemia. We also examined the neuroprotective properties of Solutol HS15, a clinically approved excipient because it possesses similar fatty acid compositions as PAME/SAME. Asphyxial cardiac arrest (ACA, 6 min) was performed 30 min after PAME/SAME treatment (0.02 mg/kg, IV). Solutol HS15 (2 ml/kg, IP) was injected chronically for 14 days (once daily). Histopathology of hippocampal CA1 neurons was assessed 7 days after ACA. For focal ischemia experiments, PAME, SAME, or Solutol HS15 was administered following reperfusion after 2 h of middle cerebral artery occlusion (MCAO). 2,3,5-Triphenyltetrazolium staining of the brain was performed 24 h after MCAO and the infarct volume was quantified. Following ACA, the number of surviving hippocampal neurons was enhanced by PAME-treated (68%), SAME-treated (69%), and Solutol-treated HS15 (68%) rats as compared to ACA only-treated groups. Infarct volume was decreased by PAME (83%), SAME (68%), and Solutol HS15 (78%) as compared to saline (vehicle) in MCAO-treated animals. PAME, SAME, and Solutol HS15 provide robust neuroprotection in both paradigms of ischemia. This may prove therapeutically beneficial since Solutol HS15 is already administered as a solublizing agent to patients. With proper timing and dosage, administration of Solutol HS15 and PAME/SAME can be an effective therapy against cerebral ischemia.

Modified application of HS-SPME for quality evaluation of essential oil plant materials.

Science.gov (United States)

Dawidowicz, Andrzej L; Szewczyk, Joanna; Dybowski, Michal P

2016-01-01

The main limitation in the standard application of head space analysis employing solid phase microextraction (HS-SPME) for the evaluation of plants as sources of essential oils (EOs) are different quantitative relations of EO components from those obtained by direct analysis of EO which was got in the steam distillation (SD) process from the same plant (EO/SD). The results presented in the paper for thyme, mint, sage, basil, savory, and marjoram prove that the quantitative relations of EO components established by HS-SPME procedure and direct analysis of EO/SD are similar when the plant material in the HS-SPME process is replaced by its suspension in oil of the same physicochemical character as that of SPME fiber coating. The observed differences in the thyme EO composition estimated by both procedures are insignificant (F(exp)HS-SPME procedure proposed in this paper substantially shortens the evaluation time of plant material quality and thus may improve the efficiency of analytical laboratories. Copyright © 2015 Elsevier B.V. All rights reserved.

Serum Pentraxin 3 and hs-CRP Levels in Children with Severe Pulmonary Hypertension

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Cemşit Karakurt

2014-09-01

Full Text Available Background: Pulmonary arterial hypertension secondary to untreated left-to-right shunt defects leads to increased pulmonary blood flow, endothelial dysfunction, increased pulmonary vascular resistance, vascular remodelling, neointimal and plexiform lesions. Some recent studies have shown that inflammation has an important role in the pathophysiology of pulmonary arterial hypertension. Aims: The aim of this study is to evaluate serum pentraxin 3 and high sensitive (hs-C reactive protein (hs-CRP levels in children with severe pulmonary arterial hypertension (PAH secondary to untreated congenital heart defects and evaluate the role of inflammation in pulmonary hypertension. Study Design: Cross sectional study. Methods: After ethics committee approval and receiving consent from parents, there were 31 children were selected for the study with severe PAH, mostly with a left-to-right shunt, who had been assessed by cardiac catheterisation and were taking specific pulmonary vasodilators. The control group consisted of 39 age and gender matched healthy children. After recording data about all the patients including age, gender, weight, haemodynamic studies and vasodilator testing, a physical examination was done for all subjects. Blood was taken from patients and the control group using peripheral veins to analyse serum Pentraxin 3, N-terminal pro-Brain Natriuretic Peptide (NT-ProBNP and hs-CRP levels. Serum Pentraxin-3 levels were measured by enzyme linked immunosorbent assay (ELISA and expressed as ng/mL. Serum hs-CRP levels were measured with an immunonephelometric method and expressed as mg/dL. The serum concentration of NT-proBNP was determined by a chemiluminescent immunumetric assay and expressed as pg/mL. Results: Serum Pentraxin- 3 levels were determined to be 1.28±2.12 (0.12-11.43 in the PAH group (group 1 and 0.40±0.72 (0.07-3.45 in group 2. There was a statistically significant difference between the two groups (p<0.01. Serum hs-CRP levels

25 CFR 1200.44 - What action will the Department take on requests for technical assistance?

Science.gov (United States)

2010-04-01

... technical assistance? 1200.44 Section 1200.44 Indians OFFICE OF THE SPECIAL TRUSTEE FOR AMERICAN INDIANS, DEPARTMENT OF THE INTERIOR AMERICAN INDIAN TRUST FUND MANAGEMENT REFORM ACT Technical Assistance § 1200.44 What action will the Department take on requests for technical assistance? We will respond in writing...

Structural and functional characterization of the interaction between cyclophilin B and a heparin-derived oligosaccharide.

Science.gov (United States)

Hanoulle, Xavier; Melchior, Aurélie; Sibille, Nathalie; Parent, Benjamin; Denys, Agnès; Wieruszeski, Jean-Michel; Horvath, Dragos; Allain, Fabrice; Lippens, Guy; Landrieu, Isabelle

2007-11-23

The chemotaxis and integrin-mediated adhesion of T lymphocytes triggered by secreted cyclophilin B (CypB) depend on interactions with both cell surface heparan sulfate proteoglycans (HSPG) and the extracellular domain of the CD147 membrane receptor. Here, we use NMR spectroscopy to characterize the interaction of CypB with heparin-derived oligosaccharides. Chemical shift perturbation experiments allowed the precise definition of the heparan sulfate (HS) binding site of CypB. The N-terminal extremity of CypB, which contains a consensus sequence for heparin-binding proteins was modeled on the basis of our experimental NMR data. Because the HS binding site extends toward the CypB catalytic pocket, we measured its peptidyl-prolyl cis-trans isomerase (PPIase) activity in the absence or presence of a HS oligosaccharide toward a CD147-derived peptide. We report the first direct evidence that CypB is enzymatically active on CD147, as it is able to accelerate the cis/trans isomerization of the Asp(179)-Pro(180) bond in a CD147-derived peptide. However, HS binding has no significant influence on this PPIase activity. We thus conclude that the glycanic moiety of HSPG serves as anchor for CypB at the cell surface, and that the signal could be transduced by CypB via its PPIase activity toward CD147.

D-Alanine-Controlled Transient Intestinal Mono-Colonization with Non-Laboratory-Adapted Commensal E. coli Strain HS.

Science.gov (United States)

Cuenca, Miguelangel; Pfister, Simona P; Buschor, Stefanie; Bayramova, Firuza; Hernandez, Sara B; Cava, Felipe; Kuru, Erkin; Van Nieuwenhze, Michael S; Brun, Yves V; Coelho, Fernanda M; Hapfelmeier, Siegfried

2016-01-01

Soon after birth the mammalian gut microbiota forms a permanent and collectively highly resilient consortium. There is currently no robust method for re-deriving an already microbially colonized individual again-germ-free. We previously developed the in vivo growth-incompetent E. coli K-12 strain HA107 that is auxotrophic for the peptidoglycan components D-alanine (D-Ala) and meso-diaminopimelic acid (Dap) and can be used to transiently associate germ-free animals with live bacteria, without permanent loss of germ-free status. Here we describe the translation of this experimental model from the laboratory-adapted E. coli K-12 prototype to the better gut-adapted commensal strain E. coli HS. In this genetic background it was necessary to complete the D-Ala auxotrophy phenotype by additional knockout of the hypothetical third alanine racemase metC. Cells of the resulting fully auxotrophic strain assembled a peptidoglycan cell wall of normal composition, as long as provided with D-Ala and Dap in the medium, but could not proliferate a single time after D-Ala/Dap removal. Yet, unsupplemented bacteria remained active and were able to complete their cell cycle with fully sustained motility until immediately before autolytic death. Also in vivo, the transiently colonizing bacteria retained their ability to stimulate a live-bacteria-specific intestinal Immunoglobulin (Ig)A response. Full D-Ala auxotrophy enabled rapid recovery to again-germ-free status. E. coli HS has emerged from human studies and genomic analyses as a paradigm of benign intestinal commensal E. coli strains. Its reversibly colonizing derivative may provide a versatile research tool for mucosal bacterial conditioning or compound delivery without permanent colonization.

25 CFR 1200.15 - What is the approval process for management plans?

Science.gov (United States)

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false What is the approval process for management plans? 1200... INTERIOR AMERICAN INDIAN TRUST FUND MANAGEMENT REFORM ACT Withdrawing Tribal Funds From Trust § 1200.15 What is the approval process for management plans? The Secretary will approve or disapprove each...

Design of the injection channel magnets for the K1200 cyclotron

International Nuclear Information System (INIS)

Wu, X.Y.; Lawton, D.; Marti, F.; Zeller, A.F.

2001-01-01

The beam from the K500 cyclotron is injected radially into the median plane of the K1200 cyclotron where it is stripped inside a dee. Along the injection path, the injected beam traverses large magnetic gradients under the K1200 superconducting coil and on the edge of the hill. To match the injected beam to the eigen-ellipse for the accelerated beam, two magnetic elements are included in the injection channel in the yoke of the K1200 cyclotron. One of them is an active element and the other a conical focusing passive element. This last element must move in the horizontal plane to match the different beam paths. We describe in the paper the beam dynamics calculations as well as the mechanical design and construction of these magnets

400-MWe consolidated nuclear steam system (CNSS): 1200-MWt/conceptual design

International Nuclear Information System (INIS)

1977-06-01

A 1200-MWt consolidated nuclear steam system (CNSS) conceptual design is described. The concept, derived from nuclear merchant ship propulsion steam systems but distinctly different from those systems in detail, incorporates the steam generators within the reactor pressure vessel. This configuration eliminates primary coolant circulating piping external to the reactor pressure vessel since the primary coolant circulating pumps are mounted in the pressure vessel head. So arranged, the maximum piping break that must be assumed is that of the pressurizer surge line, which is substantially smaller than a primary coolant circulating line. A fracture of the pressurizer surge line would result in substantially lower mass and energy release rates of the primary coolant during the assumed loss-of-coolant accident. This in turn makes practical a pressure-suppression containment rather than the ''dry'' containment commonly used for pressurized water reactors

The Role of the Media in South African Construction Health and Safety (H&S

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John Smallwood

2012-11-01

Full Text Available A large number of fatalities and injuries occur in the South African construction industry. Traditionally, the print media have dedicated editorial, published news, articles and letters, and have exposed abusive or non-conforming conditions and practices in terms of H&S. Literature also indicates that the print media can influence and has an impact on H&S.Given the level of fatalities and injuries and the potential role of the print media, a postal survey was conducted among editors of construction and related magazines. Findings indicate that: the print media do contribute to and play a role in construction H&S; industry has the capacity and needs to promote H&S on a wider basis; there is a need to improve construction H&S; to a degree, editors are aware of what constitutes unsafe acts and unsafe conditions, and the print media can play an increased role through the review of articles, advertisements, advertorial, editorial and phototgraphs to prevent the depiction of unhealthy and unsafe practices and conditions

Mechanistic study of solubility enhancement of nifedipine using vitamin E TPGS or solutol HS-15.

Science.gov (United States)

Rajebahadur, Minal; Zia, Hossein; Nues, Anthony; Lee, Chong

2006-01-01

The objective of our study was to find mechanisms responsible for solubility enhancement of nifedipine in solid dispersions of vitamin E TPGS and/or solutol HS-15. Solid dispersions of nifedipine with selected polymers such as vitamin E TPGS, solutol HS-15, PEG(1,000), and lipocol C-10 of varying drug/polymer ratios were prepared by a fusion method. The solubility enhancement was found to be in the order of vitamin E TPGS > solutol HS-15 > lipocol C-10 > PEG(1,000). Lipocol C-10, with a similar hydrophilic-lipophilic value as vitamin E TPGS, showed a comparable retained solubility enhancement during saturation solubility studies but had lower dissolution profile. Overall, vitamin E TPGS showed the best solubility and dissolution performance, while solutol HS-15 and lipocol C-10 demonstrated moderate solubility enhancements. Solid dispersions of vitamin E TPGS as prepared by microfluidization technique initially showed slightly higher solubility compared with samples prepared by fusion method, but eventually it became the same as the study progressed. However, solid dispersion of solutol HS-15 as prepared by microfluidization demonstrated a significant, sustained increased in solubility over its sample when prepared by fusion method. Based on these results, we concluded that enhanced solubility using vitamin E TPGS and solutol HS-15 resulted from a partial conversion of crystalline drug to the amorphous form, increase in wettability of the drug by water soluble polymers, better separation of drug particles, micellar solubilization of drug by high concentrations of surfactant polymers, and interaction between polymer and drug at the molecular level.

Modification of the Armijo line search to satisfy the convergence properties of HS method

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Mohammed Belloufi

2013-07-01

Full Text Available The Hestenes-Stiefel (HS conjugate gradient algorithm is a useful tool of unconstrainednumerical optimization, which has good numerical performance but no global convergence result under traditional line searches. This paper proposes a line search technique that guarantee the globalconvergence of the Hestenes-Stiefel (HS conjugate gradient method. Numerical tests are presented tovalidate the different approaches.

Evaluation of the OSI LD1200 optical diskdrive

International Nuclear Information System (INIS)

Burger, J.M.

1986-01-01

This document describes the testing of a laser-diskdrive of Optical Storage International, model LD1200. The aim was to test the hardware and media reliability and to verify some of the vendor supplied specifications. (Auth.)

40 CFR 60.1200 - What are the operating practice requirements for my municipal waste combustion unit?

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2010-07-01

... requirements for my municipal waste combustion unit? 60.1200 Section 60.1200 Protection of Environment... SOURCES Standards of Performance for Small Municipal Waste Combustion Units for Which Construction is... Good Combustion Practices: Operating Requirements § 60.1200 What are the operating practice...

49 CFR 1200.2 - Adoption of generally accepted accounting principles issued by the Financial Accounting Standards...

Science.gov (United States)

2010-10-01

... principles issued by the Financial Accounting Standards Board (FASB). 1200.2 Section 1200.2 Transportation... COMMERCE ACT § 1200.2 Adoption of generally accepted accounting principles issued by the Financial... Financial Accounting Standards by the FASB, and provided that the Office of Economics, Environmental...

Application value of Serum Hs-CRP, IL-6 and plasma FIB joint detection in COPD

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Feng Ji

2016-11-01

Full Text Available Objective: To discuss the application value of High sensitivity C-reactive protein (Hs-CRP, interleukin-6 (IL-6 and fibrinogen (FIB joint detection in chronic obstructive pulmonary disease (COPD. Methods: A total of 181 COPD cases were divided to be COPD stable phase group (65 cases and COPD acute exacerbation phase group (116 cases per the course of disease. COPD acute exacerbation phase group was classified into grade I (39 cases, grade II (43 cases and grade III (34 cases based on pulmonary function. Then survival group (87 cases and death group (29 cases were divided based on illness transition. Meanwhile, 80 cases of healthy people at the same phase were set to be healthy group. Differences in levels of Serum hs-CRP, IL-6 and FIB in these groups were analyzed, and according to these indexes, prognostic potency of COPD acute exacerbation phase could be evaluated. Results: Difference in serum hs-CRP, IL-6 and FIB levels in COPD stable phase group, COPD acute exacerbation phase group and healthy group were statistical significant (P<0.05. both for healthy group hs-CRP, IL-6 and FIB levels in grade I, II, III of pulmonary function in the COPD acute exacerbation phase group were statistical significant (P<0.05 both for grade 1 < grade 2 < grade 3. Result of person analyzing showed significant positive correlation on grading of pulmonary function and serum hs-CRP, IL-6 and FIB levels, the correlation coefficient was 0.573. Differences of hs-CRP, IL-6 and FIB levels between survival group and death group were statistical significant. Serum hs-CRP, IL-6 and FIB levels were utilized respectively to evaluate area under curve of receiver operating characteristic in prognostic COPD acute exacerbation phase group, namely, 0.836, 0.815, 0.776. Sensitivities of “death”, which was evaluated by the various indexes, respectively showed as: 72.41%, 65.51% and 75

Effect of flurbiprofen aretilon on serum hs-CRP, IL-6 levels in patients undergoing esophageal cancer surgery

International Nuclear Information System (INIS)

Li Jiakai

2011-01-01

Objective: To investigate the effect of flurbiprofen axetil on serum high sensitivity C reactive protein (hs-CRP) and interleukin-6 (IL-6) in the patients undergoing esophageal cancer surgery. Methods: Thirty patients were divided into 2 groups with 15 cases each. The patients in groups A were given flurbiprofen axetil and those in group B were not as the controls. Serum hs-CRP (immuno-turbidity method) and IL-6 (RIA) levels were determined before anesthesia induction and after extubation. Results: The levels of serum hs-CRP, IL-6 were significantly higher in group B than those in group A (P<0.05). Conclusion: Flurbiprofen axetil could reduce serum hs-CRP, IL-6 levels in patients undergoing Esophageal cancer surgery. (authors)

Speed of change in biliary lipids and bile acids with chenodeoxycholic acid--is intermittent therapy feasible?

Science.gov (United States)

Iser, J H; Murphy, G M; Dowling, R H

1977-01-01

To see whehter intermittent chenodeoxycholic acid (CDCA) therapy is a potential alternative to continous treatment for gallstone dissolution, the speed of change in bile lipid composition was studied after starting and stopping CDCA therapy. In addition, the relationship between bile lipid composition and the proportions of the bile acids was examined. Bile-rich duodenal fluid was collected twice in the first week and then at approximately weekly intervals for four to six weeks, from six gallstone patients starting 13-15 mg CDCA.kg BW-1 day-1 and from another group of six patients whose treatment was stopped after gallstone dissolution. After starting treatment, the mean biliary cholesterol saturation index (based on criteria of Hegardt and Dam, 1971) decreased from 1-49 +/- SEM 0-17 to 0-92 +/- 0-13 at three weeks and 0-88 +/- 0-10 at four weeks, by which time bile lipid composition had become relatively constant. In patients whose treatment was stopped, bile reverted to its supersaturated state within one week, changing from an on-treatment mean saturation index of 0-74 +/- 0-10 to 1-15 +/- 0-15 in six to eight days after withdrawing CDCA. The proportion of conjugated CDCA in the biliary bile acids increased from 27-9 +/- 2-5% to 60-5 +/- 4-2% within four days and to 80-7 +/- 6-2% by four weeks after starting CDCA. When treatment was stopped, the proportion of CDCA reverted to pretreatment levels by two to three weeks. The saturation index was significantly related (P less than 0-001) to the percent of conjugated CDCA present, such that when the proportion of CDCA exceeded 70%, bile was almost invariably unsaturated. Since the mean time taken for bile to become unsaturated was not shorter than the time taken for bile to revert to its supersaturated state, it seems that intermittent treatment would not be adequate to maintain an unsaturated bile and is, therefore, unlikely to be as effective as continuous treatment in dissolving gallstones. PMID:838406

Mechanism of mucosal permeability enhancement of CriticalSorb® (Solutol® HS15) investigated in vitro in cell cultures.

Science.gov (United States)

Shubber, Saif; Vllasaliu, Driton; Rauch, Cyril; Jordan, Faron; Illum, Lisbeth; Stolnik, Snjezana

2015-02-01

CriticalSorb™, with the principal component Solutol® HS15, is a novel mucosal drug delivery system demonstrated to improve the bioavailability of selected biotherapeutics. The intention of this study is to elucidate mechanism(s) responsible for the enhancement of trans-mucosal absorption of biological drugs by Solutol® HS15. Micelle size and CMC of Solutol® HS15 were determined in biologically relevant media. Polarised airway Calu-3 cell layers were used to measure the permeability of a panel of biological drugs, and to assess changes in TEER, tight junction and F-actin morphology. The rate of cell endocytosis was measured in vitro in the presence of Solutol® HS15 using a membrane probe, FM 2-10. This work initially confirms surfactant-like behaviour of Solutol® HS15 in aqueous media, while subsequent experiments demonstrate that the effect of Solutol® HS15 on epithelial tight junctions is different from a 'classical' tight junction opening agent and illustrate the effect of Solutol® HS15 on the cell membrane (endocytosis rate) and F-actin cytoskeleton. Solutol® HS15 is the principle component of CriticalSorb™ that has shown an enhancement in permeability of medium sized biological drugs across epithelia. This study suggests that its mechanism of action arises primarily from effects on the cell membrane and consequent impacts on the cell cytoskeleton in terms of actin organisation and tight junction opening.

The Value of HbA1c and hs-CRP Combined Detection in Diagnosis of Diabetic Retinopathy%HbA1c、hs-CRP 联合检测诊断糖尿病视网膜病变的价值

Institute of Scientific and Technical Information of China (English)

李孝才; 孙学芬; 吴建霞; 马雪燕

2016-01-01

目的：探讨糖化血红蛋白（HbA1c）和高敏 C 反应蛋白（hs-CRP）联合检测诊断糖尿病视网膜病变的价值。方法：选择正常体检者(A 组）、糖尿病无视网膜病变者(B 组）、糖尿病视网膜病变者(C 组）各72例，采用离子交换液相层析法和胶乳增强免疫比浊法测定 HbA1c 及 hs-CRP。结果：无视网膜病变组和视网膜病变组的 HbA1c 和 hs-CRP（mg/L）含量均比健康对照组高，具有统计学意义（P0.05）；但是 HbA1c +Hs-CRP 诊断糖尿病视网膜病变的特异为90.3%，分别与 HbA1c 和 hs-CRP 存在明显差异（P 0.05); but specific to the HbA1c of hs CRP in the diagnosis of diabetic retinopathy was 90.3%, respectively and hs CRP and HbA1c exist significant difference (P< 0.05). Conclusion:The detection of HBA1c and Hs-CRPis helpful to early detection of diabetic retinopathy, which can be used asmonitoring indicators for progress and treatment effect in type 2 diabetes mellitus patients.

Development of digital subtraction system DAR-1200

International Nuclear Information System (INIS)

Kawai, Masumi; Shimizu, Yasumitsu; Ozaki, Takeshi; Sawada, Hiroshi; Uzuyama, Kazuhiro; Nishioka, Hiroyuki

1989-01-01

Digital subtraction angiography (DSA) has been of widespread use clinically, and it has attracted considerable attention in angiographic examination today. The merits of Shimadzu high resolution digital subtraction system DAR-1200 are reported in this paper. Furthermore, the principle and clinical usefullness of a new method of DSA called the Peak-Hold DSA are explained especially in details. (author)

New portable 1200 W X-ray detectors

International Nuclear Information System (INIS)

Liebram, A.; Niemann, W.; Nielsen, J.

2005-01-01

The contribution presents new detectors of up to 1200 W. The new detectors by YXLON have several of the following advantages, depending on the application: a) Reduced exposure time; b) Higher contrast; c) deeper penetration. The high efficiency, the duty cycle achieved, and the low price make the instruments interesting also for stationary applications in radiography and radioscopy [de

Social Exclusion Index-for Health Surveys (SEI-HS): a prospective nationwide study to extend and validate a multidimensional social exclusion questionnaire.

Science.gov (United States)

van Bergen, Addi P L; Hoff, Stella J M; Schreurs, Hanneke; van Loon, Annelies; van Hemert, Albert M

2017-03-14

Social exclusion (SE) refers to the inability of certain groups or individuals to fully participate in society. SE is associated with socioeconomic inequalities in health, and its measurement in routine public health monitoring is considered key to designing effective health policies. In an earlier retrospective analysis we demonstrated that in all four major Dutch cities, SE could largely be measured with existing local public health monitoring data. The current prospective study is aimed at constructing and validating an extended national measure for SE that optimally employs available items. In 2012, a stratified general population sample of 258,928 Dutch adults completed a version of the Netherlands Public Health Monitor (PHM) questionnaire in which 9 items were added covering aspects of SE that were found to be missing in our previous research. Items were derived from the SCP social exclusion index, a well-constructed 15-item instrument developed by the Netherlands Institute for Social Research (SCP). The dataset was randomly divided into a development sample (N =129,464) and a validation sample (N = 129,464). Canonical correlation analysis was conducted in the development sample. The psychometric properties were studied and compared with those of the original SCP index. All analyses were then replicated in the validation sample. The analysis yielded a four dimensional index, the Social Exclusion Index for Health Surveys (SEI-HS), containing 8 SCP items and 9 PHM items. The four dimensions: "lack of social participation", "material deprivation", "lack of normative integration" and "inadequate access to basic social rights", were each measured with 3 to 6 items. The SEI-HS showed adequate internal consistency for both the general index and for two of four dimension scales. The internal structure and construct validity of the SEI-HS were satisfactory and similar to the original SCP index. Replication of the SEI-HS in the validation sample confirmed its

In vitro investigation on the impact of Solutol HS 15 on the uptake of colchicine into rat hepatocytes.

Science.gov (United States)

Bravo González, Roberto Carlos; Boess, Franziska; Durr, Evelyne; Schaub, Nathalie; Bittner, Beate

2004-07-26

In the current investigation, the impact of the surface-active formulation ingredient Solutol HS 15 on the uptake of colchicine into freshly isolated rat hepatocytes was investigated using a centrifugal filtration technique through a silicone oil layer. Colchicine is taken up into the cells by an active transport mechanism. When conducting the experiment at 37 degrees C, it was found that at concentrations below its critical micellar concentration (CMC) of 0.021% (0.0003 and 0.003%, w/v), Solutol HS 15 did not impact the uptake of colchicine. By contrast, at a Solutol HS 15 concentration above its CMC (0.03%, w/v), the amount of colchicine taken up into the cells as well as its uptake velocity were significantly decreased. However, in control experiments performed at 4 degrees C, a temperature at which active transport processes should be significantly slowed down, Solutol HS 15 at 0.03% did not affect colchicine uptake and/or its association with the cells. The described findings might be rationalized by inhibition of colchicine transport either due to direct interaction at the transport site or due to alterations of membrane properties in the presence of Solutol HS 15 at concentrations above its CMC. Moreover, a strong molecular interaction between Solutol HS 15 and colchicine as well as an incorporation of colchicine into micelles formed by Solutol HS 15, this way resulting in a limited contact of colchicine with the cells, cannot be excluded as contributors to the observed effect.

The carriage of potentially pathogenic β-haemolytic streptococci (β-HS in healthy adult inhabitants of Wielkopolska, Poland

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Maciej Bura

2016-09-01

Full Text Available Background . Acute pharyngitis is a common disease in primary health care patients. Beta-hemolytic streptococci (β-HS are a frequent etiological agent, while swab culture from the throat and palatine tonsils is the gold standard for diagnosis. Objectives. The evaluation of the prevalence of potentially pathogenic β-HS carriage in young adult inhabitants of Wielkopolska. Material and methods. The study included 205 healthy young adult (18–44 years old (29.0 ± 6.4 inhabitants (M – 95, F – 110 of Wielkopolska (Poland. Two swabs were taken from the throat: the first one was used to perform a rapid antigenic diagnostic test (RAD T for group A streptococci (GAS and the second to grow a conventional culture. If β-HS grew, isolates were serotyped according to Lancefield. In addition, simple demographic and medical data were collected from the subjects. The study was financed from funds granted within the first edition of the “Give a hand” project (Karol Marcinkowski Poznan University of Medical Sciences. Results . Positive throat cultures were obtained in 15.6% out of 205 persons (n = 32. The distribution of β-HS serogroup results was as follows: group C (GCS – 17 cases (53.1% of cases where β-HS was positive, group F (GFS – 9 cases (28.1%, GAS – 3 people (9.4% and group G (GGS – 3 subjects (9.4%. The growth of the discussed bacteria in cultures was moderate or high in 53% of these cases. RAD T was positive in 5 cases (but only one person had GAS in throat culture. The positive culture result was significantly more frequent in men (62.5% out of 32 cases with β-HS growth, p = 0.0460. Conclusions . 1. The possibility of β-HS carriage must be taken into account when analysing the results of throat swab cultures. 2. GAS carriage is significantly lower than non-GAS carriage. 3. GCS were the most prevalent among non-GAS. 4. Being male may predispose individuals to β-HS carriage.

Clinical diagnostic value of determination of plasma ET, Hcy and serum hs-CRP contents in patients with coronary heart diseases

International Nuclear Information System (INIS)

Chu Jingying; Zhu Xueming

2008-01-01

Objective: To study the clinical diagnostic value of determination of plasma ET, Hcy and serum hs-CRP contents in patients with coronary heart disease (CHD). Methods: Plasma ET, Hcy(with RIA) and serum hs-CRP (with immune turbidimetry) contents were determined in 38 patients with CHD and 35 controls. Results: Plasma ET, Hcy and serum hs-CRP concentration were significantly higher in CHD patients (than those in controls P<0.01). Plasma ET levels were significantly positively correlated with those of plasma Hcy and serum hs-CRP (r=0.6122, 0.5842, P<0.01). Conclusion: Detection of changes of plasma ET, Hcy and serum hs-CRP levels might be of prognostic importance in patients with CHD. (authors)

Determination of Sesquiterpenes in Wines by HS-SPME Coupled with GC-MS

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Fabrizio Cincotta

2015-07-01

Full Text Available The sesquiterpene compounds present in red wines were characterized and quantified by Headspace Solid-Phase Microextraction in combination with Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS. Sixteen sesquiterpenes were identified, mainly hydrocarbons but also derived oxygenated compounds. Sesquiterpenes were acyclic, monocyclic, byciclic and tryciclic. Sesquiterpenes were detected in SIM (selected ion monitoring mode using their characteristics ions. All the sesquiterpenes were identified by mass spectral data, linear retention indices (LRI, literature data and injection of standards where available. Quantitative results were obtained using the method of standard additions. The method showed an average LOD = 0.05 µg L−1 and LOQ = 0.15 µg L−1. The monocyclic sesquiterpene with the germacrene skeleton, Germacrene D and the bicyclic sesquiterpene with the muurolane skeleton, α-muurolene were present in all the wine samples analysed. Syrah wines were the samples richest in sesquiterpenes in agreement with their typical spicy and woody notes. The results evidenced the possibility to use sesquiterpenes for wine authenticity and traceability.

Protective activity of a novel resveratrol analogue, HS-1793, against DNA damage in 137Cs-irradiated CHO-K1 cells

International Nuclear Information System (INIS)

Jeong, Min Ho; Jo, Young Rae; Yang, Kwang Mo; Jeong, Dong Hyeok; Lee, Chang Geun; Oh, Su Jung; Jeong, Soo Kyung; Jo, Wol Soon; Lee, Ki Won

2014-01-01

Resveratrol has received considerable attention as a polyphenol with anti-oxidant, anti-carcinogenic, and anti-inflammatory effects. Radiation is an important component of therapy for a wide range of malignant conditions. However, it causes damage to normal cells and, hence, can result in adverse side effects. This study was conducted to examine whether HS-1793, a novel resveratrol analogue free from the restriction of metabolic instability and the high dose requirement of resveratrol, induces a protective effect against radiation-induced DNA damage. HS-1793 effectively scavenged free radicals and inhibited radiation-induced plasmid DNA strand breaks in an in vitro assay. HS-1793 significantly decreased reactive oxygen species and cellular DNA damage in 2 Gy-irradiated Chinese hamster ovary (CHO)-K1 cells. In addition, HS-1793 dose-dependently reduced the levels of phosphorylated H2AX in irradiated CHO-K1 cells. These results indicate that HS-1793 has chemical radioprotective activity. Glutathione levels and superoxide dismutase activity in irradiated CHO-K1 cells increased significantly following HS-1793 treatment. The enhanced biological anti-oxidant activity and chemical radioprotective activity of HS-1793 maintained survival of irradiated CHO-K1 cells in a clonogenic assay. Therefore, HS-1793 may be of value as a radioprotector to protect healthy tissue surrounding tumor cells during radiotherapy to obtain better tumor control with a higher dose. (author)

Comparative analysis of the aroma chemicals of Melissa officinalis using hydrodistillation and HS-SPME techniques

Directory of Open Access Journals (Sweden)

Shakeel-u- Rehman

2017-05-01

Full Text Available Headspace solid-phase micro extraction (HS-SPME coupled with gas chromatography–mass spectrometry (GC–MS has been used for the chemical analysis of Melissa officinalis (leaves cultivated in Institute Germplasm. The HS-SPME analysis led to the identification of 22 components constituting 99.1% of the total volatile constituents present in the leaves whereas its hydrodistillate led to the identification of 24 volatile constituents constituting 98.1% of the volatile material. The chemical composition of the SPME and hydrodistilled extract of M. officinalis leaves comprised mainly of oxygenated monoterpenes (78.5% and 57.8% respectively and sesquiterpene hydrocarbons (14.9% and 29.7% respectively. The major components identified in the HS-SPME extract were citronellal (31.1%, citronellol (18.3%, β-caryophyllene (12.0%, (E-citral (11.9%, (Z-citral (9.6%, geraniol (3.6%, (Z-β-ocimene (3.1% and 1-octen-3-ol (2.0% whereas hydrodistilled essential oil was rich in (Z-citral (19.6%, β-caryophyllene (13.2%, (E-citral (11.2%, citronellal (10.2%, germacrene-d (8.3%, δ-3-carene (5.0%, 6-methyl-5-hepten-2-one (3.7% and citronellyl acetate (3.7%. The comparative analysis of volatile constituents of M. officinalis leaf extract using HS-SPME and hydrodistillation techniques shows both qualitative as well as quantitative differences. The current study is the first report involving rapid analysis of volatile components of M. officinalis by HS-SPME.

CriticalSorb: a novel efficient nasal delivery system for human growth hormone based on Solutol HS15.

Science.gov (United States)

Illum, Lisbeth; Jordan, Faron; Lewis, Andrew L

2012-08-20

The absorption enhancing efficiency of CriticalSorb for human growth hormone (MW 22 kDa) was investigated in the conscious rat model. The principle absorption enhancing component of CriticalSorb, Solutol HS15, comprises polyglycol mono- and di-esters of 12-hydroxystearic acid combined with free polyethylene glycol. When administering hGH nasally in rats with increasing concentrations of Solutol HS15, it was found that for a 10%w/v solution formulation a bioavailability of 49% was obtained in the first 2h after administration. Furthermore it was shown that the most effective ratio of Solutol HS15 to hGH was 4:1 on a mg to mg basis. Histopathology studies in rats after 5 days repeated nasal administration showed that Solutol HS15 had no toxic effect on the nasal mucosa. These results have been confirmed in a 6 month repeat nasal toxicity study in rats. It can be concluded that the principle absorption enhancing component of CriticalSorb - Solutol HS15 - is a potent and non- toxic nasal absorption enhancer that warrants further development. Copyright © 2012 Elsevier B.V. All rights reserved.

NCBI nr-aa BLAST: CBRC-DNOV-01-1200 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-DNOV-01-1200 ref|YP_784045.1| NADH dehydrogenase subunit 1 [Scutigerella cause...yae] gb|ABF93312.1| NADH dehydrogenase subunit 1 [Scutigerella causeyae] YP_784045.1 1.6 23% ...

PHOTOMETRIC ANALYSIS OF HS Aqr, EG Cep, VW LMi, AND DU Boo

Energy Technology Data Exchange (ETDEWEB)

Djurasevic, G.; Latkovic, O. [Astronomical Observatory, Volgina 7, 11000 Belgrade (Serbia); Bastuerk, Oe.; Y Latin-Small-Letter-Dotless-I lmaz, M. [Astronomy and Space Sciences Research and Application Center, Ankara University, TR-06837 Ahlatl Latin-Small-Letter-Dotless-I bel, Ankara (Turkey); Cal Latin-Small-Letter-Dotless-I skan, S.; Senavc Latin-Small-Letter-Dotless-I , H. V.; K Latin-Small-Letter-Dotless-I l Latin-Small-Letter-Dotless-I coglu, T.; Ekmekci, F. [Department of Astronomy and Space Sciences, Faculty of Science, Ankara University, TR-06100 Tandogan, Ankara (Turkey); Tanr Latin-Small-Letter-Dotless-I verdi, T., E-mail: olivia@aob.rs [Department of Physics, Faculty of Arts and Sciences, Nigde University, 51240 Nigde (Turkey)

2013-03-15

We analyze new multicolor light curves for four close late-type binaries: HS Aqr, EG Cep, VW LMi, and DU Boo, in order to determine the orbital and physical parameters of the systems and estimate the distances. The analysis is done using the modeling code of G. Djurasevic, and is based on up-to-date measurements of spectroscopic elements. All four systems have complex, asymmetric light curves that we model by including bright or dark spots on one or both components. Our findings indicate that HS Aqr and EG Cep are in semi-detached, while VW LMi and DU Boo are in overcontact configurations.

The HS6000/20 development system

International Nuclear Information System (INIS)

Schimmel, F.

1989-04-01

In the actual control system of MEA, NIKHEF-K three color displays are in use for graphical representation of the accelerator status information. At the moment the quality of the color displays is bad. The HS6000 development system of the English company Syntel seems to offer a possible solution to replace these displays. In the development system software is developed which finally may run on a target module. The target module, consisting of a processor card with memory and RS232 port and a graphical card, takes care for the control of a color display. The institute has disposed of an experimental set up during two weeks in order to investigate the possibilities and limitations of the development system. The HS6000 development system runs under the OS-9 multi-user and multi-tasking operating system. The operating system takes care for a part of the necessary software to render a computer valuable. The OS-9 operating system disposes of about 60 utility commands. A feature of OS-9 is the application of modular software technique which are based upon memory modules. The interface between the user and the internal functions of the operating system is formed from the shell, the OS-9 command-interpreter code. The software, which has to run on the target module, is written with the help of the Mmacs screen editor, the draw program and the medit editor. The draw program and the medit editor form part of the EGOS ( Enhanced Graphical Output Software) packet. The EGOS packet is a multi-tasking graphical interface for the OS-9 operating system. (author). 1 fig

HS.Register - An Audit-Trail Tool to Respond to the General Data Protection Regulation (GDPR).

Science.gov (United States)

Gonçalves-Ferreira, Duarte; Leite, Mariana; Santos-Pereira, Cátia; Correia, Manuel E; Antunes, Luis; Cruz-Correia, Ricardo

2018-01-01

Introduction The new General Data Protection Regulation (GDPR) compels health care institutions and their software providers to properly document all personal data processing and provide clear evidence that their systems are inline with the GDPR. All applications involved in personal data processing should therefore produce meaningful event logs that can later be used for the effective auditing of complex processes. Aim This paper aims to describe and evaluate HS.Register, a system created to collect and securely manage at scale audit logs and data produced by a large number of systems. Methods HS.Register creates a single audit log by collecting and aggregating all kinds of meaningful event logs and data (e.g. ActiveDirectory, syslog, log4j, web server logs, REST, SOAP and HL7 messages). It also includes specially built dashboards for easy auditing and monitoring of complex processes, crossing different systems in an integrated way, as well as providing tools for helping on the auditing and on the diagnostics of difficult problems, using a simple web application. HS.Register is currently installed at five large Portuguese Hospitals and is composed of the following open-source components: HAproxy, RabbitMQ, Elasticsearch, Logstash and Kibana. Results HS.Register currently collects and analyses an average of 93 million events per week and it is being used to document and audit HL7 communications. Discussion Auditing tools like HS.Register are likely to become mandatory in the near future to allow for traceability and detailed auditing for GDPR compliance.

Tumor vessel normalization by the PI3K inhibitor HS-173 enhances drug delivery.

Science.gov (United States)

Kim, Soo Jung; Jung, Kyung Hee; Son, Mi Kwon; Park, Jung Hee; Yan, Hong Hua; Fang, Zhenghuan; Kang, Yeo Wool; Han, Boreum; Lim, Joo Han; Hong, Soon-Sun

2017-09-10

Tumor vessels are leaky and immature, which causes poor oxygen and nutrient supply to tumor vessels and results in cancer cell metastasis to distant organs. This instability of tumor blood vessels also makes it difficult for anticancer drugs to penetrate and reach tumors. Numerous tumor vessel normalization approaches have been investigated for improving drug delivery into tumors. In this study, we investigated whether phosphoinositide 3-kinase (PI3K) inhibitors are able to improve vascular structure and function over the prolonged period necessary to achieve effective vessel normalization. The PI3K inhibitors, HS-173 and BEZ235 potently suppressed tumor growth and hypoxia, and increased tumor apoptosis in animal models. PI3K inhibitors also induced a regular, flat monolayer of endothelial cells (ECs) in vessels, improving stability of vessel structure, and normalized tumor vessels by increasing vascular maturity, pericyte coverage, basement membrane thickness, and tight-junctions. These effects resulted in a decrease in tumor vessel tortuosity and vessel thinning, and improved vessel function and blood flow. The tumor vessel stabilization effect of the PI3K inhibitor HS-173 also decreased the number of metastatic lung nodules in vivo metastasis model. Furthermore, HS-173 improved the delivery of doxorubicin into the tumor region, enhancing its anticancer effects. Mechanistic studies suggested that PI3K inhibitor HS-173-induced vessel normalization reflected changes in endothelial Notch signaling. Taken together, our findings indicate that vessel normalization by PI3K inhibitors restrained tumor growth and metastasis while improving chemotherapy by enhancing drug delivery into the tumor, suggesting that HS-173 may have a therapeutic value as an enhancer or an anticancer drug. Copyright © 2017 Elsevier B.V. All rights reserved.

HURRICANE AND SEVERE STORM SENTINEL (HS3) FLIGHT REPORTS V1

Data.gov (United States)

National Aeronautics and Space Administration — The Hurricane and Severe Storm Sentinel (HS3) Flight Reports provide information about flights flown by the WB-57 and Global Hawk aircrafts during the Hurricane and...

Free radical reactions of isoxazole and pyrazole derivatives of hispolon: kinetics correlated with molecular descriptors.

Science.gov (United States)

Shaikh, Shaukat Ali M; Barik, Atanu; Singh, Beena G; Modukuri, Ramani V; Balaji, Neduri V; Subbaraju, Gottumukkala V; Naik, Devidas B; Priyadarsini, K Indira

2016-12-01

Hispolon (HS), a natural polyphenol found in medicinal mushrooms, and its isoxazole (HI) and pyrazole (HP) derivatives have been examined for free radical reactions and in vitro antioxidant activity. Reaction of these compounds with one-electron oxidant, azide radicals ([Formula: see text]) and trichloromethyl peroxyl radicals ([Formula: see text]), model peroxyl radicals, studied by nanosecond pulse radiolysis technique, indicated formation of phenoxyl radicals absorbing at 420 nm with half life of few hundred microseconds (μs). The formation of phenoxyl radicals confirmed that the phenolic OH is the active centre for free radical reactions. Rate constant for the reaction of these radicals with these compounds were in the order k HI ≅ k HP  >   k HS . Further the compounds were examined for their ability to inhibit lipid peroxidation in model membranes and also for the scavenging of 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical and superoxide ([Formula: see text]) radicals. The results suggested that HP and HI are less efficient than HS towards these radical reactions. Quantum chemical calculations were performed on these compounds to understand the mechanism of reaction with different radicals. Lower values of adiabatic ionization potential (AIP) and elevated highest occupied molecular orbital (HOMO) for HI and HP compared with HS controlled their activity towards [Formula: see text] and [Formula: see text] radicals, whereas the contribution of overall anion concentration was responsible for higher activity of HS for DPPH, [Formula: see text], and lipid peroxyl radical. The results confirm the role of different structural moieties on the antioxidant activity of hispolon derivatives.

Clinical significance of determination of serum hypersensitive C reactive protein (HS-CRP) levels in patients with acute coronary syndrome (ACS)

International Nuclear Information System (INIS)

Zhai Chunxi; Zhang Fengju; Wang Kejun

2005-01-01

Objective: To study the relationship between changes in serum HS-CRP levels and the status of atherosclerotic plaques in patients with ACS. Methods: Serum HS-CRP levels were measured in 35 patients with ACS at admission, 1 week and 1 month later as well as in 30 controls without recent infection. Results: HS-CRP levels in patients with ACS were significantly higher than those in the controls (P<0.01). The levels were highest at admission and fell gradually. Conclusion: HS-CRP could be a marker reflecting the status of atherosclerotic plaques in patients with ACS. (authors)

Performance Analysis of an Enhanced PRMA-HS Protocol for LEO Satellite Communication

Institute of Scientific and Technical Information of China (English)

ZHUO Yong-ning; YAN Shao-hu; WU Shi-qi

2005-01-01

The packet reservation multiple access with hindering state (PRMA-HS) is a protocol suitable for LEO satellite mobile communication. Although working well with light system payload (amount of user terminals), the protocol imposes high channel congestion on system with heavy payload, thus degrades the system's quality of service. To controlling the channel congestion, a scheme of enhanced PRMA-HS protocol is proposed, which aims to reduce the collision of voice packets by adopting a mechanism of access control. Through theoretic analysis, the system's mathematic model is presented and the packet drop probability of the scheme is deduced. To testify the performance of the scheme, a simulation is performed and the results support our analysis.

Microanalysis of oligosaccharide HS203 in beagle dog plasma by postcolumn fluorescence derivatization method.

Science.gov (United States)

Sun, Shumeng; Zhao, Xia; Li, Guangsheng; Yu, Guangli; Xing, Xiaoxu; Zeng, Yangyang; Wu, Jian; Wang, Jianing

2012-06-20

A rapid and sensitive postcolumn fluorescence derivatization method was developed for microanalysis of antidiabetic oligosaccharide HS203 in beagle dog plasma. After plasma protein was removed by a simple and fast ultrafiltration method, chromatographic separation was performed on an Asahipak GS-320 HQ column with a mobile phase of 50 mmol/L phosphate buffer (pH 6.7) and acetonitrile (83/17, v/v). The column effluent was monitored by fluorescence detection at 249 nm (excitation) and 435 nm (emission) using guanidine hydrochloride as a postcolumn derivatizing reagent. A satisfactory resolution of the analyte was achieved and the limit of detection was found to be 4 ng (more sensitive than silver staining of HS203 in polyacrylamide gel electrophoresis). The method described above was successfully applied to a pharmacokinetic study of HS203 and to monitor blood glucose level simultaneously in beagle dog. It is also possible to be applied for microanalysis of other oligosaccharides in biological samples. Copyright © 2012 Elsevier Ltd. All rights reserved.

Increased hsCRP is associated with higher risk of aortic valve replacement in patients with aortic stenosis

DEFF Research Database (Denmark)

Blyme, Adam; Nielsen, Olav W.; Asferg, Camilla

2016-01-01

Objective To investigate relations between inflammation and aortic valve stenosis (AS) by measuring high-sensitivity C-reactive protein, at baseline (hsCRP0) and after 1 year (hsCRP1) and exploring associations with aortic valve replacement (AVR). Design We examined 1423 patients from...... the Simvastatin and Ezetimibe in Aortic Stenosis study. Results During first year of treatment, hsCRP was reduced both in patients later receiving AVR (2.3 [0.9–4.9] to 1.8 [0.8–5.4] mg/l, p CRP1...... predicted later AVR (HR = 1.17, p CRP0 (HR = 0.96, p = 0.33), aortic valve area (AVA) and other risk factors. A higher rate of AVR was observed in the group with high hsCRP0 and an increase during the first year (AVRhighCRP0CRP1inc=47.3% versus AVRhighCRP0CRP1dec=27.5%, p

Impact of Solutol HS 15 on the pharmacokinetic behaviour of colchicine upon intravenous administration to male Wistar rats.

Science.gov (United States)

Bittner, Beate; González, Roberto Carlos Bravo; Walter, Isabelle; Kapps, Martin; Huwyler, Jörg

2003-05-01

In the current investigation, the alkaloid colchicine was administered intravenously to male Wistar rats both as a solution in isotonic sodium chloride (NaCl 0.9%, control group) and in NaCl 0.9%:Solutol HS 15 (95:5) at 1.5 mg/kg. At predetermined time points, plasma and urine were collected from the animals and analysed for colchicine and its demethylated metabolites by LC/MS-MS. In the presence of Solutol HS 15, colchicine clearance (CI) was significantly decreased and its maximum plasma concentration (c(max)) was significantly increased as compared to the control group (CI: 15.6+/-7.0 ml/min/kg vs 34.3+/-2.3 ml/min/kg; c(max) 3055.1+/-587.4 h vs 1260.1+/-223.7 h; pSolutol HS 15 treated group (41.50+/-3.23 vs 1.17+/-0.41% of total dose; pSolutol HS 15 compared with surfactant-free incubations (overall means: 72.25+/-0.50% for FF, 0.80+/-0.02 for lambda, 0.46+/-0.04 for K(e)). In vitro, in rat hepatocytes, the clearance of colchicine was significantly reduced at 0.003% Solutol HS 15 present in the incubation medium (0.86+/-0.15 microl/min/10(-6) cells vs 1.46+/-0.06 microl/min/10(-6) cells). As colchicine exhibits a comparatively high aqueous solubility, an impact of Solutol HS 15 on the solubility of the alkaloid is very unlikely to be a reason for the observed effect. Therefore, our results indicate that the most likely reasons for the changed pharmacokinetic behaviour of colchicine in the presence of Solutol HS 15 are alterations of metabolism and/or transport as well as distribution and elimination processes. Copyright 2003 John Wiley & Sons, Ltd.

Study of High Sensitive C-Reactive Protein (HS-CRP) After Cardiac Rehabilitation Program in Patients Undergoing Isolated CABG.

Science.gov (United States)

Johari Moghadam, Adel; Azizinejad, Saied

2016-12-01

Although cardiac rehabilitation is known as a tool to reduce the overall risk of cardiovascular complications, its specific role in the reduction of hs-CRP as a marker of inflammation and a proven marker of cardiovascular risk needs further investigation. The present study aims at elucidating the effects of a full course of conventional cardiac rehabilitation program for the period of eight weeks, on the levels of hs-CRP in patients who underwent isolated coronary artery bypass surgery. In this case study, 30 consecutive patients who underwent isolated coronary artery bypass surgery (isolated CABGS), and a full 8-week cardiac rehabilitation program in Tehran Heart Center, were investigated. A group of 30 similar patients, who enrolled in the same period of rehabilitation program but did not participate in practice, was considered as a control group. Serum levels of hs-CRP in both groups were measured retrospectively and in similar days before the start of rehabilitation program and at the end of it (or 8 weeks after initial registration for the control group). Levels of hs-CRP in the rehabilitation group and control group were 5.9 7.7 and 6.3 6.9 respectively before start of the program which was not statistically meaningful ( P -Value = 0.833). However, after the program, level of hs-CRP in the two tested groups changed to 2.3 5.1 and 5.7 6.1 respectively which showed a meaningful correlation ( P -Value = 0.023). These results also showed that decrease in hs-CRP level in the rehabilitated group but not in the control group was statistically meaningful (with P -Value of 0.037 and 0.0723 respectively). In patients undergoing coronary bypass surgery, participating in a full course of cardiac rehabilitation for 8 weeks has resulted in a significant reduction in hs-CRP levels as a marker of cardiovascular risk.

NCBI nr-aa BLAST: CBRC-OGAR-01-1200 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-OGAR-01-1200 ref|YP_885208.1| hypothetical protein MSMEG_0804 [Mycobacterium sme...gmatis str. MC2 155] gb|ABK71723.1| hypothetical protein MSMEG_0804 [Mycobacterium smegmatis str. MC2 155] YP_885208.1 0.20 42% ...

Clinical significance of measurement of serum hs-CRP, TNF-α and M-CSF levels after treatment in patients with periodontitis

International Nuclear Information System (INIS)

Zhang Yunming

2007-01-01

Objective: To explore the significance of changes of serum hs-CRP, TNF-α and M-CSF levels after treatment in patients with periodontitis. Methods: Serum TNF-α, M-CSF (with RIA), hs-CRP (with immuneturbitity method) levels were determined in 38 patients with periodontitis both before and after treatment as well as in 35 controls. Results: Before treatment, the serum hs-CRP, TNF-α and M-CSF levels were significantly higher in the patients than those in controls (P 0.05). Conclusion: Detection of serum hs-CRP, TNF-α and M-CSF levels might reflect the progress of disease in patients with periodontitis. (authors)

Mechanism of mucosal permeability enhancement of CriticalSorb® (Solutol® HS15) investigated In Vitro in cell cultures.

OpenAIRE

Shubber, Saif; Vllasaliu, Driton; Rauch, Cyril; Jordan, Faron; Illum, Lisbeth; Stolnik, Snjezana

2015-01-01

Purpose CriticalSorb?, with the principal component Solutol? HS15, is a novel mucosal drug delivery system demonstrated to improve the bioavailability of selected biotherapeutics. The intention of this study is to elucidate mechanism(s) responsible for the enhancement of trans-mucosal absorption of biological drugs by Solutol? HS15. Methods Micelle size and CMC of Solutol? HS15 were determined in biologically relevant media. Polarised airway Calu-3 cell layers were used to measure the permeab...

BN-1200 Reactor Power Unit Design Development

International Nuclear Information System (INIS)

Vasilyev, B.A.; Shepelev, S.F.; Ashirmetov, M.R.; Poplavsky, V.M.

2013-01-01

Main goals of BN-1200 design: • Develop a reliable new generation reactor plant for the commercial power unit with fast reactor to implement the first-priority objectives in changing over to closed nuclear fuel cycle; • Improve technical and economic indices of BN reactor power unit to the level of those of Russian VVER of equal power; • Enhance the safety up to the level of the requirements for the 4th generation RP

Analysis of relationship between blood lipid metabolism levels and hs-CRP levels in patients with chronic obstructive pulmonary disease

International Nuclear Information System (INIS)

You Fengjian

2011-01-01

Objective: To study the relationship between blood lipid metabolism levels and hs-CRP levels in the patients with chronic obstructive pulmonary disease. Methods: The levels of plasma blood lipid (with biochemistry) and serum hs-CRP(with high-sensitive immuno turbidimetry) were determined in 96 patients with chronic obstructive pulmonary disease as well as 68 normal controls. Results: The plasma blood lipid levels in 96 patients with chronic obstructive pulmonary disease were significantly lower than those in 68 controls, plasma TC and LDL-C levels were not much difference (P>0.05), plasma HDL-C level was significantly difference (P<0.05), but TG and Lp (a) levels were very prominently difference (P<0.01). And the plasma hs-CRP level was significantly increased also (P<0.01). The close relationship was between blood lipid and hs-CRP levels. Conclusion: The study of relationship between blood lipid levels and hs-CRP levels in patients with COPD was helpful for understand the disease process as well as possible mechanisms. (authors)

49 CFR 1200.1 - Financial statements released by carriers.

Science.gov (United States)

2010-10-01

..., except in reports to this Board, based on generally accepted accounting principles for which there is... 49 Transportation 9 2010-10-01 2010-10-01 false Financial statements released by carriers. 1200.1... TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION (CONTINUED) ACCOUNTS, RECORDS AND REPORTS GENERAL ACCOUNTING...

Effect of the additives on clouding behavior and thermodynamics of coenzyme Q10-Kolliphor HS15 micelle aqueous solutions

Science.gov (United States)

Hu, Li; Zhang, Jing; Zhu, Chao; Pan, Hong-chun; Liu, Hong

2017-11-01

Herein we investigate the effect of different additives (electrolytes, amino acids, PEG, and sugars) on the cloud points (CP) of coenzyme Q10 (CoQ10) - Kolliphor HS15 (HS15) micelle aqueous solutions. The CP values were decreased with the increase of electrolytes and sugars, following: CPAl3+ reduced the CP. A depression of CP for CoQ10-HS15 micelle solution with PEG was molecular weight of PEG dependent. The significant thermodynamic parameters were also evaluated and discussed.

The Re-Fe-S system at 1200, 1100, 1000 and 900 degree C

DEFF Research Database (Denmark)

Karup-Møller, Sven; Makovicky, E.

1999-01-01

Phase relations in the dry condensed phase system Fe-Re-S from 1200oC down to 900oC involve S-poor Fe-S melt with up to 0.2 at.% dissolved Re, S-rich melt with 0.3-0.4 at.% dissolved Re at 1200oC, Fe1-xS with Re contents growing from nil for troilite to 0.2 at.% for S-richer compositions, gFe wit...

CoCoRaHS: A Community Science Program Providing Valuable Precipitation Data to Guide Decision Making

Science.gov (United States)

Robinson, D. A.; Doesken, N.

2017-12-01

CoCoRaHS is an acronym for the Community Collaborative Rain, Hail and Snow Network. It is long-running, community-based network of volunteers working together to measure and map precipitation (rain, hail and snow). Precipitation is an ideal element for public engagement because it affects everyone, it is so variable in time and space and it impacts so many things. By using a standard precipitation gauge, stressing training and education, utilizing an interactive website, and having observations undergo quality assurance, the CoCoRaHS program provides high-quality data for natural resource, education and research applications. The program currently operates in all states, Canada and the Bahamas. It originated with the Colorado Climate Center at Colorado State University in 1998 due in part to the Fort Collins flood a year prior. Upwards of 12,000 observers submit observations each day. Observations meet federal guidelines and are archived at the U.S. National Centers for Environmental Information. Because of excellent spatial coverage, data quality, practical relevance, and accessibility, CoCoRaHS observations are used by a wide variety of organizations and individuals. The U.S. National Weather Service, hydrologists, emergency managers, city utilities (water supply, storm water), insurance adjusters, the U.S. Department of Agriculture, engineers, mosquito control commissions, ranchers and farmers, outdoor and recreation interests, teachers and students are just some examples of those who use CoCoRaHS data in making well-informed, meaningful decisions. Some examples of community applications and the science utility of CoCoRaHS observations include storm warnings, water supply and demand forecasts, disaster declarations (drought, winter storm, etc.), drought and food production assessments, calibration/validation of remote sensing, infrastructure evaluation and potential redesign (ice and snow loading, bridge, storm and sewer design), recreation planning, and

Clinical Significance of Determination of the Serum Levels of NT-proBNP and hs-CRP in Patients with Acute Coronary Syndrome

International Nuclear Information System (INIS)

Zheng Zhaojun; Zheng Jing; Sun Weili; Yuan Yuan; Tao Jian; Li Weipeng

2010-01-01

To explore the clinical significance the serum levels of N-Terminal proB-Type natriuretic peptide (NT-proBNP) and high-sensitivity C-reactive protein (hs-CRP) in patients with acute coronary syndrome,the serum levels of NT-proBNP and hs-CRP in patients and normal controls were determined by ECi Immunity Analyzer and radioimmunoassay respectively. The results showed that the serum levels of NT-proBNP and hs-CRP in patients with acute coronary syndrome were significantly higher than that of controls (P<0.05). The diagnostic specificity for acute coronary syndrome was 100% by combined detection of NT-proBNP and hs-CRP. The results suggest that the combined detection of serum NT-proBNP and hs-CRP levels are very important to evaluate heart function in patients with acute coronary syndrome. (authors)

Correlation of Serum Levels of Vitronectin, Malondialdehyde and Hs-CRP With Disease Severity in Coronary Artery Disease

Directory of Open Access Journals (Sweden)

Alireza Yaghoubi

2015-09-01

Conclusion: The association and correlation between VN, MDA and hs-CRP indicate their involvement in the atherosclerosis process that may lead to progression of CAD. Also, these findings suggested that serum levels of VN, MDA and hs-CRP can help as diagnostic and monitoring markers in CAD patients and as markers of disease severity.

Clinical application of combined detection of serum hs-CRP, GMP-140 and cTnI in patients with coronary heart diseases

International Nuclear Information System (INIS)

Qin Jibao; Wu Zhaozeng

2010-01-01

Objective: To explore the clinical significance of changes of serum hs-CRP, GMP-140 and cTnI levels in patients with coronary heart diseases. Methods: Serum GMP-140 (with RIA), cTnI (with ELISA) and hs-CRP (with immuno turbidity method) levels were determined in 91 patients with coronary heart diseases (42 SAP, 34UAP, 15AMI) and 35 controls. Results: Serum hs-CRP, GMP-140, cTnI levels in patients with coronary heart diseases were significantly higher than those in controls (P <0.01). Among the patients with of coronary heart diseases, the magnitude of changes of the levels of serum hs-CRP, GMP-140 and cTnI levels in AMI and UAP groups were significantly larger than those in SAP group (P < 0.05). Serum hs-CRP levels were positively correlated with serum GMP-140 and cTnI levels (r = 0.6214, 0.6023, P < 0.01). Conclusion: Serum hs-CRP, GMP140 and cTnI levels were closely related to the diseases process of coronary heart diseases and were of great clinical importance for assessment of the disease and outcome prediction. (authors)

Clinical significance of changes of serum hs-CRP, IL-6 and TNF-α levels after treatment in patients with polycystic ovary syndrome

International Nuclear Information System (INIS)

Yang Wen; Wang Ying

2010-01-01

Objective: To explore the clinical significance of changes of serum hs-CRP, IL-6 and TNF-α levels in patients with polycystic ovary syndrome. Methods: Serum hs-CRP (with immuno turbidity method), IL-6, TNF-α (with RIA) levels were determined in 31 patients with polycystic ovary syndrome both before and after six, month's treatment as well as 35 controls. Results: Before treatment, the serum hs-CRP, IL-6 and TNF-α levels in the 31 patients with polycystic ovary syndrome were significantly higher than those in controls (P 0.05). Serum hs-CRP levels were positive correlate with serum IL-6, TNF-α levels (r=0.6014, 0.5982, P<0.01). Conclusion: Serum hs-CRP, IL-6 and TNF-α levels were correlated to the development of polycystic ovary syndrome (PCOS). (authors)

Optimal Tilt Angle and Orientation of Photovoltaic Modules Using HS Algorithm in Different Climates of China

Directory of Open Access Journals (Sweden)

Mian Guo

2017-10-01

Full Text Available Solar energy technologies play an important role in shaping a sustainable energy future, and generating clean, renewable, and widely distributed energy sources. This paper determines the optimum tilt angle and optimum azimuth angle of photovoltaic (PV panels, employing the harmony search (HS meta-heuristic algorithm. In this study, the ergodic method is first conducted to obtain the optimum tilt angle and the optimum azimuth angle in several cities of China based on the model of Julian dating. Next, the HS algorithm is applied to search for the optimum solution. The purpose of this research is to maximize the extraterrestrial radiation on the collector surface for a specific period. The sun’s position is predicted by the proposed model at different times, and then solar radiation is obtained on various inclined planes with different orientations in each city. The performance of the HS method is compared with that of the ergodic method and other optimization algorithms. The results demonstrate that the tilt angle should be changed once a month, and the best orientation is usually due south in the selected cities. In addition, the HS algorithm is a practical and reliable alternative for estimating the optimum tilt angle and optimum azimuth angle of PV panels.

Performance of a 1200 m long suspended Fabry-Perot cavity

CERN Document Server

Freise, A; Gossler, S; Grote, H; Heinzel, G; Lück, H B; Robertson, D I; Strain, K A; Ward, H; Willke, B; Hough, J; Danzmann, K

2002-01-01

Using one arm of the Michelson interferometer and the power recycling mirror of the interferometric gravitational wave detector GEO 600, we created a Fabry-Perot cavity with a length of 1200 m. The main purpose of this experiment was to gather first experience with the main optics, its suspensions and the corresponding control systems. The residual displacement of a main mirror is about 150 nm rms. By stabilizing the length of the 1200 m long cavity to the pre-stabilized laser beam, we achieved an error point frequency noise of 100 mu Hz Hz sup - sup 1 sup / sup 2 at 100 Hz Fourier frequency. In addition we demonstrated the reliable performance of all included subsystems by several 10-hour-periods of continuous stable operation. Thus the full frequency stabilization scheme for GEO 600 was successfully tested.

Elevated levels of Hs-CRP and IL-6 after delivery are associated with depression during the 6 months post partum.

Science.gov (United States)

Liu, Hao; Zhang, Yang; Gao, Yutao; Zhang, Zhenyu

2016-09-30

The objective of this study is to determine whether inflammatory markers (high-sensitivity C-reactive protein (Hs-CRP) and interleukin (IL)-6) early in the postpartum period contribute to the development of postpartum depression (PPD). From 4 May 2014 to 30 June 2014, all eligible women not on medication for depression giving birth at the Beijing Chao-Yang hospital were consecutively recruited and followed up for 6 months. Depression symptoms were measured with the Edinburgh Postnatal Depression Scale (EPDS), and inflammatory biomarkers (Hs-CRP and IL-6) were tested. During the study period, 296 women were enrolled and completed follow-up. In these women, 45 (15.2%) were considered as meeting the criteria for PPD. Serum levels of Hs-CRP and IL-6 in women with PPD were significantly higher than those without PPD (all P<0.0001). Receiver operating characteristics to predict PPD demonstrated areas under the curve of IL-6 of 0.861 (95% confidence interval (CI), 0.801-0.922), which was superior to Hs-CRP (0.837 (95% CI, 0.781-0.894), P<0.01). In multivariate logistic regression analysis, IL-6 and Hs-CRP were independent predictors of PPD. The present study demonstrates a strong relationship between elevated serum Hs-CRP and IL-6 levels at admission and the development of PPD within 6 months. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Exploring Dust Impacts on Tropical Systems from the NASA HS-3 Field Campaign

Science.gov (United States)

Nowottnick, Ed; Colarco, Pete; da Silva, Arlindo; Barahona, Donifan; Hlavka, Dennis

2015-01-01

One of the overall scientific goals of the NASA Hurricane and Severe Storm Sentinel (HS-3) field campaign is to better understand the role of the Saharan Air Layer (SAL) in tropical storm development. During the 2012 HS-3 deployment, the Cloud Physics Lidar (CPL) observed dust within SAL air in close proximity to a developing Nadine (September 11, 2012). Throughout the mission, the NASA GEOS-5 modeling system supported HS-3 by providing 0.25 degrees resolution 5-day global forecasts of aerosols, which were used to support mission planning. The aerosol module was radiatively interactive within the GEOS-5 model, but aerosols were not directly coupled to cloud and precipitation processes. In this study we revisit the aerosol forecasts with an updated version of the GEOS-5 model. For the duration of Hurricane Nadine, we run multiday climate simulations leading up to each respective Global Hawk flight with and without aerosol direct interaction. For each set of simulations, we compare simulated dust mass fluxes to identify differences in SAL entrainment related to the interaction between dust aerosols and the atmosphere. We find that the direct effects of dust induce a low level anticyclonic circulation that temporarily shields Nadine from the intrusion of dry air, leading to a more intense storm.

Osteoprotegerin improves risk detection by traditional cardiovascular risk factors and hsCRP

DEFF Research Database (Denmark)

Mogelvang, Rasmus; Haahr-Pedersen, Sune Ammentorp; Bjerre, Mette

2013-01-01

To evaluate the association of plasma osteoprotegerin (OPG) to hospitalisation for ischaemic heart disease (IHD), ischaemic stroke and all-cause mortality, and the effect of combining plasma OPG and high-sensitivity C-reactive protein (hsCRP)....

Clinical significance of determination of serum hypersensitive C-reactive protein (hs-CRP) and E-selectin levels in patients with coronary heart diseases

International Nuclear Information System (INIS)

Yang Chunxiu

2007-01-01

Objective: To investigate the significance of determination of serum contents of hs-CRP and E-Selectin in patients with coronary heart diseases (CHD). Methods: Serum hs-CRP Contents were determined with immuno-turbidity and E-Selectin contents were determined with ELISA in 58 patients with CHD (35SAP, 20UAP, 13AMI) and 35 controls. Results: Serum levels of hs-CRP and E-Selectin in CHD patients were significantly higher than those in controls (P 0.05). Conclusion: The serum levels of hs-CRP and E-Selectin were correlated to the development of CHD, but not to the coronary artery calibers. (authors)

Survivability and growth kinetics of methanogenic archaea at various pHs and pressures: Implications for deep subsurface life on Mars

Science.gov (United States)

Sinha, Navita; Nepal, Sudip; Kral, Timothy; Kumar, Pradeep

2017-02-01

Life as we know it requires liquid water and sufficient liquid water is highly unlikely on the surface of present-day Mars. However, according to thermal models there is a possibility of liquid water in the deep subsurface of Mars. Thus, the martian subsurface, where the pressure and temperature is higher, could potentially provide a hospitable environment for a biosphere. Also, methane has been detected in the Mars' atmosphere. Analogous to Earth's atmospheric methane, martian methane could also be biological in origin. The carbon and energy sources for methanogenesis in the subsurface of Mars could be available by downwelling of atmospheric CO2 into the regolith and water-rock reactions such as serpentinization, respectively. Corresponding analogs of the martian subsurface on Earth might be the active sites of serpentinization at depths where methanogenic thermophilic archaea are the dominant species. Methanogens residing in Earth's hydrothermal environments are usually exposed to a variety of physiological stresses including a wide range of pressures, temperatures, and pHs. Martian geochemical models imply that the pH of probable groundwater varies from 4.96 to 9.13. In this work, we used the thermophilic methanogen, Methanothermobacter wolfeii, which grows optimally at 55oC. Therefore, a temperature of 55oC was chosen for these experiments, possibly simulating Mars' subsurface temperature. A martian geophysical model suggests depth and pressure corresponding to a temperature of 55 °C would be between 1-30 km and 100-3,000 atm respectively. Here, we have simulated Mars deep subsurface pH, pressure, and temperature conditions and have investigated the survivability, growth rate, and morphology of M. wolfeii after exposure to a wide range of pH 5-9) and pressure (1-1200 atm) at a temperature of 55 °C. Interestingly, in this study we have found that M. wolfeii was able to survive at all the pressures and pHs tested at 55 °C. In order to understand the effect of

The Value of PCT, WBC and hs-CRP Tests in the Diagnosis and Prognosis of Children with Pneumonia%小儿肺炎中PCT、WBC、hs-CRP检测对疾病诊断及预后判断价值分析

Institute of Scientific and Technical Information of China (English)

夏兰兰; 李敬

2017-01-01

目的 分析小儿肺炎中PCT、WBC、hs-CRP检测对疾病诊断及预后的判断价值.方法 选取2015年5月至2016年4月在本院住院治疗的肺炎患儿120例作为观察组,健康儿童65例作为对照组.检测治疗前后患儿及健康儿童血清PCT、WBC、hs-CRP的变化.结果 治疗前,细菌感染组WBC数量(15.5±3.4)×109/L、hs-CRP水平(19.8±2.6) mg/L和PCT水平(21.9±2.1)ng/mL均明显高于非细菌感染组(6.8 ±2.3)×109/L、(4.5±2.3)mg/L、(4.7±1.8) ng/mL和健康对照组(8.1 ±1.4)×109/L、(3.5±1.2) mg/L、(1.8±0.5) ng/mL,且差异具有统计学意义(P＜0.05).而非细菌感染组和健康对照组的WBC、hs-CRP和PCT结果差异无统计学意义(P＞0.05);治疗后,细菌感染组WBC数量(7.3±1.4)×109/L、hs-CRP水平(5.1 ± 1.2) mg/L和PCT水平(2.3 ± 1.2) ng/mL均明显低于治疗前,差异具有统计学意义(P＜0.05);WBC、hs-CRP、PCT联合检测的灵敏性95.2％、特异性93.0％、准确度93.3％显著高于WBC、hs-CRP、PCT单独检测,差异具有统计学意义(P＜0.05).结论 在小儿肺炎治疗过程中,动态监测患儿血清的WBC、hs-CRP、PCT的变化,并结合临床,有助于诊疗和判断患儿疾病愈后的情况,具有显著的临床意义,值得推广应用.%Objective To estimate the value of PCT,WBC and hs-CRP tests in the diagnosis and prognosis of children with pneumonia.Methods 120 cases with pneumonia in children were selected from May 2015 to April 2016 in our hospital as the observation group,65 cases of healthy children as the control group.Changes of serum PCT,WBC and hs-CRP tests in children and healthy children before and after treatment were mearued.Results Before treatment,bacterial infection group have WBC count (15.5 ± 3.4) × 109/L,hs-CRP levels (19.8±2.6) mg/L and PCT level (21.9 ±2.1) ng/mL,which were significantly higher than those in non bacterial infection group (6.8 ±2.3) × 109/L,(4.5 ±2.3) mg/L,(4.7 ± 1.8) ng/mL and healthy control group (+ 8.1 �

High-sensitivity c-reactive protein (hs-CRP) value with 90 days mortality in patients with heart failure

Science.gov (United States)

Nursyamsiah; Hasan, R.

2018-03-01

Hospitalization in patients with chronic heart failure is associated with high rates of mortality and morbidity that during treatment and post-treatment. Despite the various therapies available today, mortality and re-hospitalization rates within 60 to 90 days post-hospitalization are still quite high. This period is known as the vulnerable phase. With the prognostic evaluation tools in patients with heart failure are expected to help identify high-risk individuals, then more rigorous monitoring and interventions can be undertaken. To determine whether hs-CRP have an impact on mortality within 90 days in hospitalized patients with heart failure, an observational cohort study was conducted in 39 patients with heart failure who were hospitalized due to worsening chronic heart failure. Patients were followed for up to 90 days after initial evaluation with the primary endpoint is death. Hs-CRP value >4.25 mg/L we found 70% was dead and hs-CRP value <4.25 mg/L only 6.9% was dead whereas the survival within 90 days. p:0.000.In conclusion, there were differences in hs-CRP values between in patients with heart failure who died and survival within 90 days.

HS-173, a Novel PI3K Inhibitor, Attenuates the Activation of Hepatic Stellate Cells in Liver Fibrosis

Science.gov (United States)

Son, Mi Kwon; Ryu, Ye-Lim; Jung, Kyung Hee; Lee, Hyunseung; Lee, Hee Seung; Yan, Hong Hua; Park, Heon Joo; Ryu, Ji-Kan; Suh, Jun–Kyu; Hong, Sungwoo; Hong, Soon-Sun

2013-01-01

Hepatic stellate cells (HSCs) are the primary source of matrix components in liver disease such as fibrosis. Phosphatidylinositol 3-kinase (PI3K) signaling in HSCs has been shown to induce fibrogenesis. In this study, we evaluated the anti-fibrotic activity of a novel imidazopyridine analogue (HS-173) in human HSCs as well as mouse liver fibrosis. HS-173 strongly suppressed the growth and proliferation of HSCs and induced the arrest at the G2/M phase and apoptosis in HSCs. Furthermore, it reduced the expression of extracellular matrix components such as collagen type I, which was confirmed by an in vivo study. We also observed that HS-173 blocked the PI3K/Akt signaling pathway in vitro and in vivo. Taken together, HS-173 suppressed fibrotic responses such as cell proliferation and collagen synthesis by blocking PI3K/Akt signaling. Therefore, we suggest that this compound may be an effective therapeutic agent for ameliorating liver fibrosis through the inhibition of PI3K signaling. PMID:24326778

Correlation of hs-CRP with environmental risk factors of nephropathy in type 2 diabetes

Directory of Open Access Journals (Sweden)

Jay Prakash Sah

2015-06-01

Full Text Available The objective of the present study was to investigate the association of hs-CRP levels with environmental risk factors of diabetic nephropathy like smoking, drinking alcohol, diet, age of diabetic patient, duration of diabetes, medication of diabetes, and blood pressure medication. A hospital-based quantitative study was conducted at the Department of Clinical Biochemistry of Manipal Teaching Hospital (MTH Pokhara, Nepal, with 89 patients suffering from type 2 diabetes. Blood samples (n=89 from the patients were collected and the serums were separated. On the other hand, data on environmental risk factors of nephropathy were collected by using standard questionnaire. In this study, serum hs-CRP level was not found to be correlated with smoking (p=0.111, alcohol consumption (p=0.722, diet (p=0.496, duration of diabetes (p=0.519, age of diabetic patient (p=0.369, medication of diabetes (p=0.734, and blood pressure medication (p=0.625. Hence, our study concludes that serum hs-CRP value in type 2 diabetic patients is insignificantly correlated with the risk factors especially smoking, drinking alcohol, diet, duration of diabetes, age of diabetic patient, medication of diabetes, and medication of blood pressure.

Lowering the quantification limit of the QubitTM RNA HS assay using RNA spike-in.

Science.gov (United States)

Li, Xin; Ben-Dov, Iddo Z; Mauro, Maurizio; Williams, Zev

2015-05-06

RNA quantification is often a prerequisite for most RNA analyses such as RNA sequencing. However, the relatively low sensitivity and large sample consumption of traditional RNA quantification methods such as UV spectrophotometry and even the much more sensitive fluorescence-based RNA quantification assays, such as the Qubit™ RNA HS Assay, are often inadequate for measuring minute levels of RNA isolated from limited cell and tissue samples and biofluids. Thus, there is a pressing need for a more sensitive method to reliably and robustly detect trace levels of RNA without interference from DNA. To improve the quantification limit of the Qubit™ RNA HS Assay, we spiked-in a known quantity of RNA to achieve the minimum reading required by the assay. Samples containing trace amounts of RNA were then added to the spike-in and measured as a reading increase over RNA spike-in baseline. We determined the accuracy and precision of reading increases between 1 and 20 pg/μL as well as RNA-specificity in this range, and compared to those of RiboGreen(®), another sensitive fluorescence-based RNA quantification assay. We then applied Qubit™ Assay with RNA spike-in to quantify plasma RNA samples. RNA spike-in improved the quantification limit of the Qubit™ RNA HS Assay 5-fold, from 25 pg/μL down to 5 pg/μL while maintaining high specificity to RNA. This enabled quantification of RNA with original concentration as low as 55.6 pg/μL compared to 250 pg/μL for the standard assay and decreased sample consumption from 5 to 1 ng. Plasma RNA samples that were not measurable by the Qubit™ RNA HS Assay were measurable by our modified method. The Qubit™ RNA HS Assay with RNA spike-in is able to quantify RNA with high specificity at 5-fold lower concentration and uses 5-fold less sample quantity than the standard Qubit™ Assay.

Three body abrasion of laser surface alloyed aluminium AA1200

CSIR Research Space (South Africa)

Mabhali, Luyolo AB

2012-06-01

Full Text Available Laser surface alloying of aluminium AA1200 was performed with a 4 kW Nd:YAG laser to improve the abrasion wear resistance. Aluminium surfaces reinforced with metal matrix composites and intermetallic phases were achieved. The phases present depended...

HURRICANE AND SEVERE STORM SENTINEL (HS3) HURRICANE IMAGING RADIOMETER (HIRAD) V1

Data.gov (United States)

National Aeronautics and Space Administration — The Hurricane and Severe Storm Sentinel (HS3) Hurricane Imaging Radiometer (HIRAD) was collected by the Hurricane Imaging Radiometer (HIRAD), which was a multi-band...

Association of HS6ST3 gene polymorphisms with obesity and ...

Indian Academy of Sciences (India)

Supplementary data: Association of HS6ST3 gene polymorphisms with obesity and triglycerides: gene × gender interaction. Ke-Sheng Wang, Liang Wang, Xuefeng Liu and Min Zeng. J. Genet. 92, 395–402. Table 1. Associations of 117 SNPs with obesity in the Health ABC and Marshfield samples. Minor. OR Health.

Development of a Direct Headspace Collection Method from Arabidopsis Seedlings Using HS-SPME-GC-TOF-MS Analysis

Directory of Open Access Journals (Sweden)

Kazuki Saito

2013-04-01

Full Text Available Plants produce various volatile organic compounds (VOCs, which are thought to be a crucial factor in their interactions with harmful insects, plants and animals. Composition of VOCs may differ when plants are grown under different nutrient conditions, i.e., macronutrient-deficient conditions. However, in plants, relationships between macronutrient assimilation and VOC composition remain unclear. In order to identify the kinds of VOCs that can be emitted when plants are grown under various environmental conditions, we established a conventional method for VOC profiling in Arabidopsis thaliana (Arabidopsis involving headspace-solid-phase microextraction-gas chromatography-time-of-flight-mass spectrometry (HS-SPME-GC-TOF-MS. We grew Arabidopsis seedlings in an HS vial to directly perform HS analysis. To maximize the analytical performance of VOCs, we optimized the extraction method and the analytical conditions of HP-SPME-GC-TOF-MS. Using the optimized method, we conducted VOC profiling of Arabidopsis seedlings, which were grown under two different nutrition conditions, nutrition-rich and nutrition-deficient conditions. The VOC profiles clearly showed a distinct pattern with respect to each condition. This study suggests that HS-SPME-GC-TOF-MS analysis has immense potential to detect changes in the levels of VOCs in not only Arabidopsis, but other plants grown under various environmental conditions.

Association of serum hs-CRP and lipids with obesity in school children in a 12-month follow-up study in Japan.

Science.gov (United States)

Nishide, Ritsuko; Ando, Michiko; Funabashi, Hidemitsu; Yoda, Yoshiko; Nakano, Masataka; Shima, Masayuki

2015-03-01

To investigate the association of serum lipids and high-sensitivity C-reactive protein (hs-CRP) with obesity in school children and to explore whether hs-CRP levels could be used to predict the presence or absence of obesity 12 months later. The subjects were school children (6-11 years old) in Japan. Blood sampling and physical measurements were performed in school (2001); low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides, and hs-CRP levels were measured. Data from children who could be followed 12 months later were analyzed. Subjects weighing 20% or more over his/her standard weight were regarded as obese, and the association of obesity with serum parameters was analyzed. Data from 612 subjects were analyzed (follow-up rate, 75.4%). The mean of each serum parameter was significantly higher (inverse for HDL-C; lower) in obese than that in non-obese children. Logistic regression analysis for obesity at baseline showed that the odds ratio (OR) of hs-CRP was the highest [OR, 2.15; 95% confidence interval (CI), 1.65-2.78 for an interquartile rage (IQR) increase]; the association with triglycerides and LDL-C/HDL-C was significant. At the 12-month follow-up, the OR of high hs-CRP remained the highest of all serum parameters (2.09; 95% CI, 1.63-2.69 for an IQR increase). High levels of triglycerides, LDL-C/HDL-C, and hs-CRP increased the risk of obesity in school children. Hs-CRP is considered to be a better predictor of obesity 12 months later than is LDL-C/HDL-C.

Fe(II)/Cu(II) interaction on goethite stimulated by an iron-reducing bacteria Aeromonas Hydrophila HS01 under anaerobic conditions.

Science.gov (United States)

Tao, Liang; Zhu, Zhen-Ke; Li, Fang-Bai; Wang, Shan-Li

2017-11-01

Copper is a trace element essential for living creatures, but copper content in soil should be controlled, as it is toxic. The physical-chemical-biological features of Cu in soil have a significant correlation with the Fe(II)/Cu(II) interaction in soil. Of significant interest to the current study is the effect of Fe(II)/Cu(II) interaction conducted on goethite under anaerobic conditions stimulated by HS01 (a dissimilatory iron reduction (DIR) microbial). The following four treatments were designed: HS01 with α-FeOOH and Cu(II) (T1), HS01 with α-FeOOH (T2), HS01 with Cu(II) (T3), and α-FeOOH with Cu(II) (T4). HS01 presents a negligible impact on copper species transformation (T3), whereas the presence of α-FeOOH significantly enhanced copper aging contributing to the DIR effect (T1). Moreover, the violent reaction between adsorbed Fe(II) and Cu(II) leads to the decreased concentration of the active Fe(II) species (T1), further inhibiting reactions between Fe(II) and iron (hydr)oxides and decelerating the phase transformation of iron (hydr)oxides (T1). From this study, the effects of the Fe(II)/Cu(II) interaction on goethite under anaerobic conditions by HS01 are presented in three aspects: (1) the accelerating effect of copper aging, (2) the reductive transformation of copper, and (3) the inhibition effect of the phase transformation of iron (hydr)oxides. Copyright © 2017 Elsevier Ltd. All rights reserved.

Transient analysis and leakage detection algorithm using GA and HS algorithm for a pipeline system

Energy Technology Data Exchange (ETDEWEB)

Kim, Sang Hyun; Yoo, Wan Suk; Oh, Kwang Jung; Hwang, In Sung; Oh, Jeong Eun [Pusan National University, Pusan (Korea, Republic of)

2006-03-15

The impact of leakage was incorporated into the transfer functions of the complex head and discharge. The impedance transfer functions for the various leaking pipeline systems were also derived. Hydraulic transients could be efficiently analyzed by the developed method. The simulation of normalized pressure variation using the method of characteristics and the impulse response method shows good agreement to the condition of turbulent flow. The leak calibration could be performed by incorporation of the impulse response method with Genetic Algorithm (GA) and Harmony Search (HS). The objective functions for the leakage detection can be made using the pressure-head response at the valve, or the pressure-head or the flow response at a certain point of the pipeline located upstream from the valve. The proposed method is not constrained by the Courant number to control the numerical dissipation of the method of characteristics. The limitations associated with the discreteness of the pipeline system in the inverse transient analysis can be neglected in the proposed method.

Transient analysis and leakage detection algorithm using GA and HS algorithm for a pipeline system

International Nuclear Information System (INIS)

Kim, Sang Hyun; Yoo, Wan Suk; Oh, Kwang Jung; Hwang, In Sung; Oh, Jeong Eun

2006-01-01

The impact of leakage was incorporated into the transfer functions of the complex head and discharge. The impedance transfer functions for the various leaking pipeline systems were also derived. Hydraulic transients could be efficiently analyzed by the developed method. The simulation of normalized pressure variation using the method of characteristics and the impulse response method shows good agreement to the condition of turbulent flow. The leak calibration could be performed by incorporation of the impulse response method with Genetic Algorithm (GA) and Harmony Search (HS). The objective functions for the leakage detection can be made using the pressure-head response at the valve, or the pressure-head or the flow response at a certain point of the pipeline located upstream from the valve. The proposed method is not constrained by the Courant number to control the numerical dissipation of the method of characteristics. The limitations associated with the discreteness of the pipeline system in the inverse transient analysis can be neglected in the proposed method

Collective dipole motion in highly excited (272)Hs (Z=108) nuclei

NARCIS (Netherlands)

Tveter, TS; Gaardhoje, JJ; Maj, A; Ramsay, T; Atac, A; Bacelar, J; Bracco, A; Buda, A; Camera, F; Herskind, B; Korten, W; Krolas, W; Menthe, A; Million, B; Nifenecker, H; Pignanelli, M; Pinston, JA; vanderPloeg, H; Schussler, F; Sletten, G

1996-01-01

The heavy nucleus (272)(108)Hs (Z = 108) and its evaporation daughters were produced using the reaction Th-232(Ar-40, gamma xn) with beam energies 10.5 and 15.0 MeV/A. The giant dipole resonance gamma radiation from the hot composite system prior to fission has been isolated using a differential

3-O-sulfated heparan sulfate recognized by the antibody HS4C3 contributes [corrected] to the differentiation of mouse embryonic stem cells via fas signaling.

Directory of Open Access Journals (Sweden)

Kazumi Hirano

Full Text Available Maintenance of self-renewal and pluripotency in mouse embryonic stem cells (mESCs is regulated by the balance between several extrinsic signaling pathways. Recently, we demonstrated that heparan sulfate (HS chains play important roles in the maintenance and differentiation of mESCs by regulating extrinsic signaling. Sulfated HS structures are modified by various sulfotransferases during development. However, the significance of specific HS structures during development remains unclear. Here, we show that 3-O-sulfated HS structures synthesized by HS 3-O-sulfotransferases (3OSTs and recognized by the antibody HS4C3 increase during differentiation of mESCs. Furthermore, expression of Fas on the cell surface of the differentiated cells also increased. Overexpression of the HS4C3-binding epitope in mESCs induced apoptosis and spontaneous differentiation even in the presence of LIF and serum. These data showed that the HS4C3-binding epitope was required for differentiation of mESCs. Up-regulation of the HS4C3-binding epitope resulted in the recruitment of Fas from the cytoplasm to lipid rafts on the cell surface followed by activation of Fas signaling. Indeed, the HS4C3-binding epitope interacted with a region that included the heparin-binding domain (KLRRRVH of Fas. Reduced self-renewal capability in cells overexpressing 3OST resulted from the degradation of Nanog by activated caspase-3, which is downstream of Fas signaling, and was rescued by the inhibition of Fas signaling. We also found that knockdown of 3OST and inhibition of Fas signaling reduced the potential for differentiation into the three germ layers during embryoid body formation. This is the first demonstration that activation of Fas signaling is mediated by an increase in the HS4C3-binding epitope and indicates a novel signaling pathway for differentiation in mESCs.

3-O-sulfated heparan sulfate recognized by the antibody HS4C3 contributes [corrected] to the differentiation of mouse embryonic stem cells via fas signaling.

Science.gov (United States)

Hirano, Kazumi; Sasaki, Norihiko; Ichimiya, Tomomi; Miura, Taichi; Van Kuppevelt, Toin H; Nishihara, Shoko

2012-01-01

Maintenance of self-renewal and pluripotency in mouse embryonic stem cells (mESCs) is regulated by the balance between several extrinsic signaling pathways. Recently, we demonstrated that heparan sulfate (HS) chains play important roles in the maintenance and differentiation of mESCs by regulating extrinsic signaling. Sulfated HS structures are modified by various sulfotransferases during development. However, the significance of specific HS structures during development remains unclear. Here, we show that 3-O-sulfated HS structures synthesized by HS 3-O-sulfotransferases (3OSTs) and recognized by the antibody HS4C3 increase during differentiation of mESCs. Furthermore, expression of Fas on the cell surface of the differentiated cells also increased. Overexpression of the HS4C3-binding epitope in mESCs induced apoptosis and spontaneous differentiation even in the presence of LIF and serum. These data showed that the HS4C3-binding epitope was required for differentiation of mESCs. Up-regulation of the HS4C3-binding epitope resulted in the recruitment of Fas from the cytoplasm to lipid rafts on the cell surface followed by activation of Fas signaling. Indeed, the HS4C3-binding epitope interacted with a region that included the heparin-binding domain (KLRRRVH) of Fas. Reduced self-renewal capability in cells overexpressing 3OST resulted from the degradation of Nanog by activated caspase-3, which is downstream of Fas signaling, and was rescued by the inhibition of Fas signaling. We also found that knockdown of 3OST and inhibition of Fas signaling reduced the potential for differentiation into the three germ layers during embryoid body formation. This is the first demonstration that activation of Fas signaling is mediated by an increase in the HS4C3-binding epitope and indicates a novel signaling pathway for differentiation in mESCs.

Detection significance of serum hs-CRP and ALB in premature infants with early infections%血清hs-CRP与ALB检测对早产儿早发感染的临床意义

Institute of Scientific and Technical Information of China (English)

王陈裕; 何海雅; 汤梦倩; 杜姗; 金燕芬; 洪艳; 胡荣全

2017-01-01

OBJECTIVE To explore the clinical diagnostic values of serum high sensitivity C-reactive protein (hs-CRP) and albumin (ALB) in premature infants with early infections .METHODS A total of 156 cases of premature infants hospitalized from Feb .2015 to Mar .2016 in our hospital for treatment were selected as the research ob-jects ,and were divided into infection group (74 cases) and non-infected group (82 cases) according to the infection status of premature infants .Blood samples of premature infants were collected for detection of white blood cell count (WBC) ,platelet count (PLT) and hs-CRP and ALB at 24h after the infants admitted into the neonatal in-tensive care unit .RESULTS There were 74 cases of premature infants had infections in the 156 cases of patients , and the infection rate was 47 .44％ ,which was mainly pneumonia .Totally 90 strains of pathogens were detected in infected premature infants ,including 18 strains of gram-negative bacteria accounting for 20 .00％ ,mainly Esche-richia coli ,and 65 strains of gram-positive bacteria accounting for 72 .22％ ,mainly Staphylococcus aurous and Staphylococcus epidermises .The white blood cell count ,platelet count and albumin of infection group were (13 . 28 ± 4 .20)× 109/L ,(238 .30 ± 41 .02)× 109/L and (26 .54 ± 10 .25)g/L ,which were lower than (17 .10 ± 5 .12) × 109/L ,(261 .05 ± 42 .06) × 109/L and (29 .64 ± 12 .24)g/L in non-infected group ,and hs-CRP and ALB in in-fection group at 24h and 48h were (2 .38 ± 0 .49)mg/L and (22 .03 ± 9 .36)mg/L ,which were significantly higher than (1 .02 ± 0 .21)mg/L and (17 .65 ± 8 .67) mg/L in non-infected group (P<0 .05) .There were 3 ca-ses died in infection group after 48h ,and the mortality rate was 4 .05％ .CONCLUSION Serum hs-CRP and ALB can be used as effective clinical diagnostic indexes of early infection in premature infants ,and hs-CRP and ALB of infants within 24 hours of admission to the NICU can greatly improve the diagnostic sensitivity .%�

30 CFR 75.1200-1 - Additional information on mine map.

Science.gov (United States)

2010-07-01

... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Maps § 75.1200-1 Additional... symbols; (g) The location of railroad tracks and public highways leading to the mine, and mine buildings... permanent base line points coordinated with the underground and surface mine traverses, and the location and...

Comparison of solutol HS 15, Cremophor EL and novel ethoxylated fatty acid surfactants as multidrug resistance modification agents.

Science.gov (United States)

Buckingham, L E; Balasubramanian, M; Emanuele, R M; Clodfelter, K E; Coon, J S

1995-08-09

Some well-known fatty acid ester surfactants, e.g., Cremophor EL and Solutol HS 15, are modulators of multidrug resistance in vitro and in vivo. Because they are polydisperse, and their active component(s) have not been identified, the therapeutic potential of such surfactants is unclear. To better define the active components of Solutol HS 15 and to make more potent surfactant multidrug resistance modulators, highly purified C-18 fatty acids were esterified with ethylene oxide at 5-200 molar ratios. Unexpectedly, ethylene oxide esters of pure 12-hydroxy stearic acid, the major components of Solutol HS 15, displayed negligible resistance modification activity compared with Solutol HS 15 itself or to stearic and oleic acid esters synthesized under identical conditions. Since oleic acid esters appeared to have good activity, a series of these compounds was prepared to determine the optimal ethylene oxide/fatty acid ratio. The optimal ratio was found to be 20 mole ethylene oxide: I mole fatty acid, with a steep decline in activity for products made with ratios above and below the optimum. The most active oleic acid ester, designated CRL 1337, was 8.4-fold as potent as Solutol HS 15 and over 19-fold as potent as Cremophor EL in promoting rhodamine 123 accumulation in multidrug-resistant KB 8-5-11 cells in vitro. Our results show that the structure of the hydrophobic domain (fatty acid) of surfactants as well as its hydrophile-lipophile balance are critical in determining the potency of surfactants as reversing agents.

Clinical significance of measurment of changes of serum IGF-I, hs-CRP levels after treatment in patients with hyperthyroidism and hypothyroidism

International Nuclear Information System (INIS)

Zhu Conggang

2009-01-01

Objective: To explore the clinical significance of changes of serum IGF-I,hs-CRP levels after treatment in patients with hyperthyroidism and hypothyroidism. Methods: Serum IGF-I, hs-CRP(with RIA), and serum hs-CRP with immuno-turbidity methods levels were determined both before and after treatment in 36 cases of hyperthyroidism 30 cases of hypothyroidism as well as 35 controls. Results: Before treatment, the serum IGF-I levels in patients with hyperthyroidism were significantly higher and the levels in patients with hypothyroidism were significantly lower than those in controls (both P<0.01). After treatment for 3 months,the levels were much corrected, but still remained significantly different from those in controls (P<0.05). Before treatment serum hs-CRP levels in both groups of patients were significantly higher than those in controls (P<0.01), but after treatment,the levels were not much different from those in controls. Conclusion: Serum levels of IGF-I and hs-CRP were closely related to the disease process in patients with hyperthyroidism as well as in patients with hypothyroidism, and were much corrected after trentment.(authors)

Exploring the smallest active fragment of HsQSOX1b and finding a highly efficient oxidative engine.

Directory of Open Access Journals (Sweden)

Wenyun Zheng

Full Text Available Human quiescin-sulfhydryl oxidase 1 isoform b (HsQSOX1b is a highly efficient, multiple-domain enzyme that directly inserts disulfide bonds into client protein. However, previous studies have focused mainly on the catalytic activity of the whole protein rather than its domain structure. In this research, we dissected the structure and function of HsQSOX1b and explored its mechanism as a highly efficient sulfhydryl oxidase by analyzing the truncated variants. The results showed that the first HsQSOX1b thioredoxin domain was essential for thiol oxidase activity. The smallest active fragment (SAQ was identified to consist of a helix-rich region (HRR and an essential for respiration and viability/augmenter of liver regeneration (ERV/ALR domain, which remained highly active to oxidize an artificial non-thiol substrate but not small molecular and protein thiols. Our study clearly demonstrated that SAQ is a highly efficient oxidative engine, which shows high efficiency in the de novo disulfide formation and oxygen reduction and that this more efficient oxidative engine is necessary for the highly efficient catalysis of QSOXs compared to Erv1 and Erv2. This study will help address the roles of different HsQSOX1b domains in de novo disulfide formation and encourage the engineering of more efficient QSOX variants for the in vitro folding of disulfide-containing proteins.

Clinical significance of determination of changes of serum hs-CRP, sICAM-1 and TNF-α levels in patients with gestational diabetes mellitus

International Nuclear Information System (INIS)

Yu Qiuyue

2009-01-01

Objective: To study the relationship between changes of serum sensitive C-reactive protein (hs-CRP), tumor necrosis factor-alpha (TNF-α) and soluble intercellular adhesion molecule-1 (sICAM-1) levels and development of the disease in patients with gestational diabetes mellitus(GDM). Methods: Serum levels of TNF-α and sICAM-1(with RIA) and hs-CRP(with immunoturbidimetry) were measured in 30 patients with GDM and 30 normal pregnant women as controls. Results: The serum hs-CRP, sICAM-1 and TNF-α contents in the patients with GDM were all significantly higher than those in normal pregnant women (all P<0.01).The serum hs-CRP levels were mutually positivety correlated with TNF-α and sICAM-1 levels (r=0.6097, 0.7213, all P<0.01). Conclusion: Determination of changes of serum hs-CRP, TNF-α and sICAM-1 levels in patients with gestational diabetes mellitus would be helpful for outcome prediction. (authors)

Storms in a lagoon: the flooding history of Schokland (Noordoostpolder, The Netherlands) during the last 1200 years derived from geological and historical archives

Science.gov (United States)

Van den Biggelaar, Don; Kluiving, Sjoerd; Van Balen, Ronald; Kasse, Kees

2013-04-01

Flevoland (central part of the Netherlands) is an area of long-term discontinuous deposition, which has been reclaimed from the Zuiderzee in the 20th century. The Zuiderzee was an inlet of the North Sea, which came into existence during Medieval times at the expense of vast peat swamps. The inhabitants of the Zuiderzee area have a long tradition of living and struggling with water. For Schokland, located in the northern part of Flevoland (The Netherlands) and its inhabitants, this tradition started at around AD 800 when peat formation came to an end in the former Flevo lagoon region. Since that time, a surficial clay cover was deposited on Schokland. The top of the peat underlying the clay is dated at ~1200 BP, while the top is dated up to ~70 BP, implying that the clay was deposited during the Medieval to Recent occupation of the former Flevo lagoon region. We have studied the clay sequence with the combined use of geological and historical archives in order to determine the most recent flooding history of Flevoland (last 1200 years). Field data, lab analyses (grainsize anlyses, TOC, pollen, microfossils) and a literature study show that storm events had a major impact on both the sedimentary and the anthropogenic history of Schokland. A comparison between geological data and documented historical storms is made and possible remnants of the eleven major storm events, mentioned in the historical sources, that eroded parts of Schokland are identified in the subsurface of the area in the form of spikes with coarser sediment relative to the background sedimentation.

The impact of diurnal fasting during Ramadan on the lipid profile, hs-CRP, and serum leptin in stable cardiac patients.

Science.gov (United States)

Khafaji, Hadi Abdul Ridha Hadi; Bener, Abdulbari; Osman, Mohammed; Al Merri, Ajayeb; Al Suwaidi, Jassim

2012-01-01

To study the effect of strict prolonged fasting on lipid profile, serum leptin, and high- sensitivity C-reactive protein (hs-CRP) in patients with different stable cardiac illnesses and look for associated new cardiac events and any correlation between entire variables. A total of 56 patients of different stable cardiac illnesses were followed in our cardiology outpatient for 3 months. Data concerning their ability to fast were collected: New York Heart Association class of congestive cardiac failure, angina class, previous myocardial infarction, previous coronary artery bypass graft, percutaneous coronary intervention, severity of valvular lesion, metallic prosthetic valve, and traditional risk factors (diabetes mellitus, insulin requirement, hypertension, hypercholesterolemia, smoking habit, and obesity). Detailed clinical examination and electrocardiography were performed in all patients in three consecutive visits before, during, and after Ramadan. Echocardiographic and angiographic findings and medication plans were collected from patient records. Lipid profile, serum leptin, and hs-CRP were assessed before, during, and after Ramadan. All patients fasted during Ramadan: 80.4% were male, 67.9% were aged >50 years, 71.4% had no change in their symptoms during fasting while 28.6% felt better. No patient has deteriorated. 91.1% of the patients were compliant with medicine during Ramadan, 73.2% after. 89.3% were compliant with diet during Ramadan with no significant change in body weight in the follow-up period. No cardiac or noncardiac morbidity or mortality was reported. High- density lipoprotein-cholesterol (HDL-C) decreased significantly during compared to before fasting (P = 0.012). Low-density lipoprotein-cholesterol (LDL-C) significantly increased during compared to before fasting (P = 0.022). No statistically significant changes were observed in total cholesterol (TC), triglycerides (TG), serum leptin, or hs-CRP. Significant correlation was observed between

Automatic magnetic susceptibility measurements between 4 K and 1200 K

International Nuclear Information System (INIS)

Raphael, G.

1969-01-01

We give a detailed description of a Faraday magnetic susceptibility balance which operates from 4 K to 1200 K. Some preliminary results on platinum and tantalum illustrate the precision and the sensitivity of the measurements. The apparatus has been designed for measurements on the plutonium compounds which present severe health hazards. (author) [fr

Clinical significance of measurement of changes of serum hs-CRP, IL-6, IL-8 M-CSF levels after treatment in patients with endometriosis

International Nuclear Information System (INIS)

Chen Xiaochao; Zhou Dongxia; Zhang Limin; Liu Hongshu

2008-01-01

Objective: To explore the significance of changes of serum hs-CRP, IL-6, IL-8 and M-CSF levels after treatment in patients with endometriosis. Methods: Serum IL-6, IL-8, M -CSF(with RIA), hs-CRP(with immuneturbidity method)levels were determined in 33 patients with endometriosis both before and after treatment as well as in 35 controls. Results: Before treatment, the serum hs-CRP, IL-6, IL-8 and M-CSF levels were significantly higher in the patients than those in controls (P 0.05). Conclusion: Detection of serum hs-CRP, IL-6, IL-8 and M-CSF levels might reflect the progress of diseases in patients with endometriosis. (authors)

Streptomyces flavogriseus HS1: isolation and characterization of extracellular proteases and their compatibility with laundry detergents.

Science.gov (United States)

Ghorbel, Sofiane; Kammoun, Maher; Soltana, Hala; Nasri, Moncef; Hmidet, Noomen

2014-01-01

The present study describes the isolation of a new protease producing Streptomyces strain HS1 and the biochemical characterization of the secreted proteases. By sequencing of its noted 16S rDNA, HS1 strain was found to have a 100% identity with Streptomyces flavogriseus. The highest protease production was found using FermII media. In these conditions maximum protease production (99 U/mL) was obtained after 96 h incubation at 30°C and 150 rpm. HS1 strain produced at least five proteases as revealed by zymogram technique. The enzyme preparation exhibited activity over a broad range of pH (5-11) and temperature (25-70°C). Optimum activity was observed at a pH of 7.0 and a temperature of 50°C. Proteolytic activity was significantly unaffected by Ca(2+) and Mg(2+). EDTA and PMSF highly decreased the original activity. The crude extracellular proteases showed high stability when used as a detergent additive. These properties offer an interesting potential for enzymatic hydrolysis at the industrial level.

2 CFR 1.200 - Purpose of chapters I and II.

Science.gov (United States)

2010-01-01

... (and thereby implement the Federal Financial Assistance Management Improvement Act of 1999, Pub. L. 106... Introduction toSubtitle A § 1.200 Purpose of chapters I and II. (a) Chapters I and II of subtitle A provide OMB... procedures for management of the agencies' grants and agreements. (b) There are two chapters for publication...

基于HS-Clustering的风电场机组分组功率预测%Wind Power Forecasting for Clustering Wind Turbines Based on HS-Clustering

Institute of Scientific and Technical Information of China (English)

高小力; 张智博; 田启明; 刘永前

2017-01-01

为了寻求风电场功率预测精度和计算效率二者的平衡,提出了一种基于霍普金斯统计量与聚类算法(HS-Clustering)的风电场机组分组功率预测方法,该方法将霍普金斯统计量与聚类算法的优势有效结合,采用霍普金斯统计量确定场内机组分组个数,通过聚类算法识别不同机组的相似性将风电场分成不同的机组群,然后对每组机群分别建立功率预测模型,从而叠加得到整场输出功率;另外以实测风速、实测功率及二者组合作为机组分组模型输入,分析其对预测精度的影响程度.实例分析表明基于HS-Clustering的分组预测方法可以显著提高预测精度,同时保证较高的计算效率;风速是影响分组效果的主要因素,对于某些分组模型,功率又可以作为风速的重要补充.%In order to balance the forecast accuracy and computational efficiency, a wind power forecasting method for clustering wind turbines is proposed based on effective combination of Hopkins statistics (HS) and clustering methods, in which Hopkins Statistics is used to determine the clustering number of a wind farm, and wind turbines in a wind farm are clustered into several groups according to the identifying of similar characteristics by clustering method.Then power forecasting model of each clustering group is built separately, whose power output is added to obtain whole power output of the wind farm.In addition, the real-time monitoring wind speed, power output and their combination are taken as the inputs for clustered group model, and their influences on the accuracy of clustering forecast model are analyzed.The case analysis shows that the HS-Clustering based forecasting method can effectively forecast the output power of the whole wind farm with better accuracy and higher computational efficiency, wind speed is the main factor affecting clustering results, and wind power can be regarded as an important additional factor as to certain

P-HS-SFM: a parallel harmony search algorithm for the reproduction of experimental data in the continuous microscopic crowd dynamic models

Science.gov (United States)

Jaber, Khalid Mohammad; Alia, Osama Moh'd.; Shuaib, Mohammed Mahmod

2018-03-01

Finding the optimal parameters that can reproduce experimental data (such as the velocity-density relation and the specific flow rate) is a very important component of the validation and calibration of microscopic crowd dynamic models. Heavy computational demand during parameter search is a known limitation that exists in a previously developed model known as the Harmony Search-Based Social Force Model (HS-SFM). In this paper, a parallel-based mechanism is proposed to reduce the computational time and memory resource utilisation required to find these parameters. More specifically, two MATLAB-based multicore techniques (parfor and create independent jobs) using shared memory are developed by taking advantage of the multithreading capabilities of parallel computing, resulting in a new framework called the Parallel Harmony Search-Based Social Force Model (P-HS-SFM). The experimental results show that the parfor-based P-HS-SFM achieved a better computational time of about 26 h, an efficiency improvement of ? 54% and a speedup factor of 2.196 times in comparison with the HS-SFM sequential processor. The performance of the P-HS-SFM using the create independent jobs approach is also comparable to parfor with a computational time of 26.8 h, an efficiency improvement of about 30% and a speedup of 2.137 times.

Benchmarking (Code2Code) of the 1Hs 3-Bladed Onshore VAWT

DEFF Research Database (Denmark)

Galinos, Christos; Schmidt Paulsen, Uwe

This study is part of the Inflow project. In this report the Nenuphar’s onshore 3-bladed Vertical Axis Wind Turbine (VAWT) prototype (1HS) is modelled in HAWC2 aeroelastic code. In the first part the model properties are summarized. Then the analysis is focused on the rotor performance and various...

HS and E Application Technology branch progress report, July 1982-July 1983

International Nuclear Information System (INIS)

Deitesfeld, C.A.

1984-01-01

This report summarizes the applied research activities of the Health, Safety and Environment (HS and E) Application Technology branch at Rocky Flats Plant from July 1982 to July 1983. In this report, major headings present studies in three fields: general safety, radiation safety and environment, and health and safety engineering and meteorological studies

Impact of Solutol HS 15 on the pharmacokinetic behavior of midazolam upon intravenous administration to male Wistar rats.

Science.gov (United States)

Bittner, Beate; González, Roberto Carlos Bravo; Isel, Hughes; Flament, Christophe

2003-07-01

The pharmacokinetic profile of midazolam (MDZ) and its major metabolites 1'-OH-midazolam (1'OH-MDZ) and 4-OH-midazolam (4OH-MDZ) was investigated in rats. MDZ was administered intravenously at 5 mg/kg either in the absence (NaCl 0.9%, control group) or in the presence of the surfactant Solutol HS 15, a weak inhibitor of cytochrome P450 3A (CYP3A) activity in vitro (Solutol HS 15-treated group). It was found that the pharmacokinetic profiles of MDZ, 1'OH-MDZ and 4OH MDZ did not differ significantly in the two dosing vehicles (P values above 0.2). MDZ exhibited a high plasma clearance (Cl) of 79 and 92 ml/min/kg (corresponding to a blood Cl of 64 and 75 ml/min/kg), a high volume of distribution (V(d)) of 4.0 and 3.6 l/kg, and an area under the plasma concentration-time curve (AUC(t0-tinf)) of 1062 and 932 h.ng/ml in the control group and in the Solutol HS 15-treated group, respectively. The amount of MDZ excreted unchanged into urine was below 0.01% with both dosing vehicles. AUC(t0-tinf) in the control group was 12.3 h.ng/ml for 1'OH-MDZ and 38.8 h.ng/ml 4OH-MDZ. In the Solutol HS 15-treated group, AUC(t0-tinf) was 14 h.ng/ml for 1'OH-MDZ and 35.4 h.ng/ml for 4OH-MDZ. The metabolite concentrations excreted into urine were below the limit of quantification. In the rat, MDZ has a high blood clearance that is limited by liver blood flow. Therefore, weak CYP3A inhibitors like Solutol HS 15 are not likely to affect the hepatic blood clearance of MDZ in vivo.

RHTF 2, a 1200 MWe high temperature reactor

International Nuclear Information System (INIS)

Brisbois, Jacques

1978-01-01

After having adapted to French conditions the 1160 MWe G.A.C. reactor, Commissariat a l'Energie Atomique and French Industry have decided to design an High Temperature Reactor 1200 MWe based on the G.A.C. technology and taking into account the point of view of Electricite de France and the experience of C.E.A. and industry on the gas cooled reactor technology. The main objective of this work is to produce a reactor design having a low technical risk, good operability, with an emphasis on the safety aspects easing the licensing problems

Conceptual core designs for a 1200 MWe sodium cooled fast reactor

International Nuclear Information System (INIS)

Joo, H. K.; Lee, K. B.; Yoo, J. W.; Kim, Y. I.

2008-01-01

The conceptual core design for a 1200 MWe sodium cooled fast reactor is being developed under the framework of the Gen-IV SFR development program. To this end, three core concepts have been tested during the development of a core concept: a core with an enrichment split fuel, a core with a single-enrichment fuel with a region-wise varying clad thickness, and a core with a single-enrichment fuel with non-fuel rods. In order to optimize a conceptual core configuration which satisfies the design targets, a sensitivity study of the core design parameters has been performed. Two core concepts, the core with an enrichment-split fuel and the core with a single-enrichment fuel with a region-wise varying clad thickness, have been proposed as the candidates of the conceptual core for a 1200 MWe sodium cooled fast reactor. The detailed core neutronic, fuel behavior, thermal, and safety analyses will be performed for the proposed candidate core concepts to finalize the core design concept. (authors)

Large Area Silicon Carbide Vertical JFETs for 1200 V Cascode Switch Operation

Directory of Open Access Journals (Sweden)

Victor Veliadis

2008-01-01

Full Text Available SiC VJFETs are excellent candidates for reliable high-power/temperature switching as they only use pn junctions in the active device area where the high-electric fields occur. VJFETs do not suffer from forward voltage degradation, exhibit excellent short-circuit performance, and operate at 300°C. 0.19 cm2 1200 V normally-on and 0.15 cm2 low-voltage normally-off VJFETs were fabricated. The 1200-V VJFET outputs 53 A with a forward drain voltage drop of 2 V and a specific onstate resistance of 5.4 mΩ cm2. The low-voltage VJFET outputs 28 A with a forward drain voltage drop of 3.3 V and a specific onstate resistance of 15 mΩ cm2. The 1200-V SiC VJFET was connected in the cascode configuration with two Si MOSFETs and with a low-voltage SiC VJFET to form normally-off power switches. At a forward drain voltage drop of 2.2 V, the SiC/MOSFETs cascode switch outputs 33 A. The all-SiC cascode switch outputs 24 A at a voltage drop of 4.7 V.

75 FR 47631 - Trinity Tank Car, Inc., a Subsidiary of Trinity Industries, Inc., Plants #19, 1200, 1017, 1110...

Science.gov (United States)

2010-08-06

... workers of Trinity Tank Car, Inc., Plant 19, Longview, Texas. The notice was published in the Federal... integrated with the production of four other affiliate facilities in Longview, Texas: Plants 1200, 1017, 1110...., Plants 19, 1200, 1017, 1110 and 1194, Longview, Texas who became totally or partially separated from...

Preparation and pharmacokinetic evaluation of curcumin solid dispersion using Solutol® HS15 as a carrier.

Science.gov (United States)

Seo, Sang-Wan; Han, Hyo-Kyung; Chun, Myung-Kwan; Choi, Hoo-Kyun

2012-03-15

Solubility of curcumin at physiological pH was significantly increased by forming solid dispersion (SD) with Solutol® HS15. Since curcumin undergoes hydrolytic degradation, chemical stability study was conducted in pH 1.2, 6.8 and 7.4 buffer media. Solutol® HS15 exhibited superior stabilizing effect to Cremophor® RH40 and Kollidon® 30. The physical state of the dispersed curcumin in the polymer matrix was characterized by differential scanning calorimetry and X-ray diffraction studies. SD preparation transformed curcumin into amorphous form and facilitated micellar incorporation, thereby preventing hydrolysis in aqueous medium. In vitro drug release in pH 6.8 buffer revealed that SD (1:10) improved the dissolution of curcumin with approximately 90% release of the drug within 1h. Pharmacokinetic study of the solid dispersion formulation in rat showed that bioavailability of the drug was significantly improved as compared to pure curcumin. SD containing 1:10 ratio of drug and Solutol® HS15 resulted in approximately 5 fold higher AUC(0-12h). SD formulation was physically stable over the study period of 3 months. Copyright © 2011 Elsevier B.V. All rights reserved.

Association of HS6ST3 gene polymorphisms with obesity and triglycerides: gene x gender interaction.

Science.gov (United States)

Wang, Ke-Sheng; Wang, Liang; Liu, Xuefeng; Zeng, Min

2013-12-01

The heparan sulfate 6-O-sulfotransferase 3 (HS6ST3) gene is involved in heparan sulphate and heparin metabolism, and has been reported to be associated with diabetic retinopathy in type 2 diabetes.We hypothesized that HS6ST3 gene polymorphisms might play an important role in obesity and related phenotypes (such as triglycerides). We examined genetic associations of 117 single-nucleotide polymorphisms (SNPs) within the HS6ST3 gene with obesity and triglycerides using two Caucasian samples: the Marshfield sample (1442 obesity cases and 2122 controls), and the Health aging and body composition (Health ABC) sample (305 cases and 1336 controls). Logistic regression analysis of obesity as a binary trait and linear regression analysis of triglycerides as a continuous trait, adjusted for age and sex, were performed using PLINK. Single marker analysis showed that six SNPs in the Marshfield sample and one SNP in the Health ABC sample were associated with obesity (P triglycerides in the Marshfield sample (P triglycerides in the Marshfield sample. These findings contribute new insights into the pathogenesis of obesity and triglycerides and demonstrate the importance of gender differences in the aetiology.

Development and validation of the International Hidradenitis Suppurativa Severity Score System (IHS4), a novel dynamic scoring system to assess HS severity

DEFF Research Database (Denmark)

Zouboulis, C C; Tzellos, T; Kyrgidis, A

2017-01-01

BACKGROUND: A validated tool for the dynamic severity assessment of hidradenitis suppurativa/acne inversa (HS) is lacking. OBJECTIVES: To develop and validate a novel dynamic scoring system to assess the severity of HS. METHODS: A Delphi voting procedure was conducted among the members......, as well as examination for correlation (Spearman's rho) and agreement (Cohen's kappa) with existing scores, were engaged to recognize the variables for a new International HS4 (IHS4) that was established by a second Delphi round. RESULTS: Consensus HS4 was based on number of skin lesions, number of skin....... Three candidate scores were presented to the second Delphi round. The resulting IHS4 score is arrived at by the number of nodules (multiplied by 1) plus the number of abscesses (multiplied by 2) plus the number of draining tunnels (multiplied by 4). A total score of 3 or less signifies mild, 4...

Towards commercial fast breeder reactors the first 1200 MWe unit

International Nuclear Information System (INIS)

Banal, M.; Carle, R.

The public probably thinks of these fast breeder reactors in terms of their rising unit capacity: RAPSODIE 20 MW (thermal), raised to 40 MW, PHENIX 25 MWe, and now 1200 MWe. However, the purposes of the project and the framework of construction have been fundamentally different in each case. Design parameters and the development program of the LMFBR are presented. (auth)

Phase diagram of the system Ca–Ti–O at 1200 K

Indian Academy of Sciences (India)

Phase relations in the system Ca–Ti–O have been established by equilibration of several samples at 1200 K for prolonged periods and identification of phases in quenched samples by optical and scanning electron microscopy, XRD and EDS. Samples representing 20 compositions in the ternary system were analyzed.

Transitioning high sensitivity cardiac troponin I (hs-cTnI) into routine diagnostic use: More than just a sensitivity issue

LENUS (Irish Health Repository)

Lee, Graham R

2016-04-01

High sensitivity cardiac troponin T and I (hs-cTnT and hs-cTnI) assays show analytical, diagnostic and prognostic improvement over contemporary sensitive cTn assays. However, given the importance of troponin in the diagnosis of myocardial infarction, implementing this test requires rigorous analytical and clinical verification across the total testing pathway. This was the aim of this study.

The influence of alkali fatty acids on the properties and the stability of parenteral O/W emulsions modified with solutol HS 15.

Science.gov (United States)

Buszello, K; Harnisch, S; Müller, R H; Müller, B W

2000-03-01

Arachis oil based parenteral O/W emulsions were prepared using soya bean phosphatidylcholine (SPC) and different combinations of co-emulsifiers containing polyethylene glycol fatty acid esters (Solutol HS 15) and alkali fatty acids (sodium laurate, sodium stearate). The parameters measured were droplet size (both by photon correlation spectroscopy and laser diffractometry), pH and zeta potential. All emulsions were subjected to autoclaving. The addition of polyethylene glycol 12-hydroxy stearate (Solutol HS 15) led to a significant decrease of mean oil droplet size. For long-term stability the amount added turned out to be the most important factor. With increased amounts of Solutol HS 15 the packing density of the emulsifier layer and the zeta potential decreased leading to instability. The optimum load of Solutol HS 15 was found to be 15 micromol/ml. Alkali fatty acids markedly improved the physical stability of the emulsions. Improved stability properties conferred to emulsions by alkali fatty acids could be attributed to the zeta potential increase even in the presence of Solutol HS 15. Consequently a mixed emulsifier film was established in which the ionized fatty acids determined the interface charge. In addition to this a strengthening of the molecular interactions occurring between phospholipid and Solutol HS 15 emulsifier in the presence of ionized fatty acids at the O/W interface can be assumed (L. Rydhag, The importance of the phase behaviour of phospholipids for emulsion stability, Fette Seifen Anstrichm. 81 (1979) 168-173). Different co-emulsifier mixtures were shown to have a pronounced impact on the plasma protein adsorption onto emulsion droplets.

Modal Identification in an Automotive Multi-Component System Using HS 3D-DIC

Directory of Open Access Journals (Sweden)

Ángel Jesús Molina-Viedma

2018-02-01

Full Text Available The modal characterization of automotive lighting systems becomes difficult using sensors due to the light weight of the elements which compose the component as well as the intricate access to allocate them. In experimental modal analysis, high speed 3D digital image correlation (HS 3D-DIC is attracting the attention since it provides full-field contactless measurements of 3D displacements as main advantage over other techniques. Different methodologies have been published that perform modal identification, i.e., natural frequencies, damping ratios, and mode shapes using the full-field information. In this work, experimental modal analysis has been performed in a multi-component automotive lighting system using HS 3D-DIC. Base motion excitation was applied to simulate operating conditions. A recently validated methodology has been employed for modal identification using transmissibility functions, i.e., the transfer functions from base motion tests. Results make it possible to identify local and global behavior of the different elements of injected polymeric and metallic materials.

Modal Identification in an Automotive Multi-Component System Using HS 3D-DIC.

Science.gov (United States)

Molina-Viedma, Ángel Jesús; López-Alba, Elías; Felipe-Sesé, Luis; Díaz, Francisco A

2018-02-05

The modal characterization of automotive lighting systems becomes difficult using sensors due to the light weight of the elements which compose the component as well as the intricate access to allocate them. In experimental modal analysis, high speed 3D digital image correlation (HS 3D-DIC) is attracting the attention since it provides full-field contactless measurements of 3D displacements as main advantage over other techniques. Different methodologies have been published that perform modal identification, i.e., natural frequencies, damping ratios, and mode shapes using the full-field information. In this work, experimental modal analysis has been performed in a multi-component automotive lighting system using HS 3D-DIC. Base motion excitation was applied to simulate operating conditions. A recently validated methodology has been employed for modal identification using transmissibility functions, i.e., the transfer functions from base motion tests. Results make it possible to identify local and global behavior of the different elements of injected polymeric and metallic materials.

Modal Identification in an Automotive Multi-Component System Using HS 3D-DIC

Science.gov (United States)

López-Alba, Elías; Felipe-Sesé, Luis; Díaz, Francisco A.

2018-01-01

The modal characterization of automotive lighting systems becomes difficult using sensors due to the light weight of the elements which compose the component as well as the intricate access to allocate them. In experimental modal analysis, high speed 3D digital image correlation (HS 3D-DIC) is attracting the attention since it provides full-field contactless measurements of 3D displacements as main advantage over other techniques. Different methodologies have been published that perform modal identification, i.e., natural frequencies, damping ratios, and mode shapes using the full-field information. In this work, experimental modal analysis has been performed in a multi-component automotive lighting system using HS 3D-DIC. Base motion excitation was applied to simulate operating conditions. A recently validated methodology has been employed for modal identification using transmissibility functions, i.e., the transfer functions from base motion tests. Results make it possible to identify local and global behavior of the different elements of injected polymeric and metallic materials. PMID:29401725

Reduction of Pu(V) and Np(V) by leonardite humic acids and their quinonoid-enriched derivatives

International Nuclear Information System (INIS)

Shcherbina, N.S.; Kalmykov, St.N.; Perminova, I.V.; Kovalenko, A.N.

2005-01-01

Full text of publication follows: Humic substances (HS) are natural poly-electrolytes ubiquitous in aquatic environment responsible for complexation of metal ions. The presence of phenolic and quinonoid moieties in the structure of HS provide for their ability to take part in redox interactions. Capability of HS to reduce plutonium from higher oxidation states (Pu(V) and Pu(VI)) to tetravalent state was reported in several studies. However, the disparate results were reported for Np(V). The contradicting results on the redox behavior of HS could originate from the structural differences of the humic materials tested, in particular, from varying content of the redox-active quinonoid moieties. To test this hypothesis, the goal of this research was to evaluate reducing performance of leonardite humic acids and of their quinonoid-enriched derivatives with respect to Pu(V) and Np(V). The quinonoid-enriched humic derivatives were obtained using the reaction of formaldehyde co-poly-condensation between parent humic material - leonardite humic acid - and model dihydroxybenzenes - hydroquinone, catechol and 1,4-benzoquinone. The humic material: quinonoid monomer ration of 1 g per 250 mg was used. The reduction of Np(V) was studied at micro- and macro-concentration level: 10 -7 M and 10 -4 M, respectively. In case of Pu(V) the concentration was about 10 -10 M. The HS concentrations varied from 1 to 100 ppm, while ionic strength was zero. All experiments were conducted in anaerobic conditions and in the darkness. The kinetics of Pu(V) reduction was studied using solvent extraction (TTA in toluene); to monitor Np(V) reduction two independent techniques were used: solvent extraction and VIS-NIR spectrophotometry. The latter allows measuring absorbance of NpO 2 + and Np-humate complexes at 981.3 and 987.4 nm, respectively. Fast reduction of Pu(V) by the parent humic material was observed and the reduction rate increased with a decrease in pH. In case of Np(V), there was no

Comprehensive evaluation of the Berthold LB1200 survey meter

International Nuclear Information System (INIS)

Davis, M.W.

1986-05-01

The performance of two Berthod LB1200 survey meters was evaluated under the influence of a range of beta and photon radiation energies and environmental conditions likely to be encountered in the field. The survey meters responded satisfactorily to the range of beta particle and photon radiation energies emitted by most radioisotopes commonly used in Canada. The survey meters performed acceptably under most of the environmental conditions tested here with the exception of radiofrequency interference and electrostatic charge

Effects of a single 1200-mg preoperative dose of gabapentin on anxiety and memory.

Science.gov (United States)

Adam, F; Bordenave, L; Sessler, D I; Chauvin, M

2012-10-01

Gabapentin has antihyperalgesic and potential anxiolytic effects. We therefore evaluated the effects of gabapentin premedication on anxiety, amnesia, and sedation. We tested the primary hypothesis that 1200mg of oral gabapentin 2 to 3h before surgery reduces preoperative anxiety. Our secondary hypothesis was that gabapentin administration is sedative without causing preoperative amnesia. Prospective, randomized and placebo-controlled study. Surgical patients having general anaesthesia were randomly assigned to either 1200mg oral gabapentin (n=32) or an identical-looking placebo (n=32) 2 to 3h before anaesthesia. Anxiety, sedation, and amnesia were quantified before premedication, 2h thereafter, and postoperatively. Preoperative anxiety was measured using the Spielberger state trait anxiety inventory (STAI state) and the visual analogue scale anxiety (VAS). Memory was assessed with the picture recall test of Snodgrass and Vanderwart. Results were compared with t, Mann-Whitney U, or Chi(2) tests as appropriate, Psedation scores. Gabapentin premedication, 1200mg, provided preoperative anxiolysis without causing sedation or impairing preoperative memory. Copyright © 2012 Société française d’anesthésie et de réanimation (Sfar). Published by Elsevier SAS. All rights reserved.

A comparison of osteoprotegerin with adiponectin and high-sensitivity C-reactive protein (hsCRP) as a marker for insulin resistance.

LENUS (Irish Health Repository)

O'Sullivan, Eoin P

2013-01-01

Insulin resistance (IR) is associated with low adiponectin and elevated high sensitivity C-reactive protein (hsCRP). Osteoprotegerin (OPG) has been shown to be elevated in type 2 diabetes, but whether it reflects underlying IR is unclear. We aimed to compare the ability of serum OPG with adiponectin and hsCRP to act as a marker for IR in individuals with normal and abnormal glucose tolerance.

Clinical significance of determination of changes of serum IGF-II, IL-6, IL-8 and hs-CRP levels after treatment in pediatric patients with bronchopneumonia

International Nuclear Information System (INIS)

Wang Guanghui; Chen Chuanbing; Wang Xianwu

2009-01-01

Objective: To explore the clinical significance of changes of serum IGF-II, IL-6, IL-8 and hs-CRP levels after treatment in pediatric patients with bronchopneumonia. Methods: Serum IGF-II, IL-6, IL-8 (with RIA) and hs-CRP (with immunoturbidity method) levels were determined in 36 pediatric patients with bronchopneumonia both before and after treatment as well as in 35 controls. Results: Before treatment, serum IGF-II, IL-6, IL-8 and hs-CRP levels in the patients were significantly higher than those in the controls (P 0.05). Conclusion: Determination of serum IGF-II, IL-6, IL-8 and hs-CRP levels in pediatric patients with bronchopneumonia was important for diagnosis and outcome prediction. (authors)

NMR, HS-SPME-GC/MS, and HPLC/MSn Analyses of Phytoconstituents and Aroma Profile of Rosmarinus eriocalyx.

Science.gov (United States)

Bendif, Hamdi; Miara, Mohamed Djamel; Peron, Gregorio; Sut, Stefania; Dall'Acqua, Stefano; Flamini, Guido; Maggi, Filippo

2017-10-01

In this work, a comprehensive study on the chemical constituents of the aerial parts of Rosmarinus eriocalyx (Lamiaceae), an aromatic shrub traditionally consumed as a food and herbal remedy in Algeria, is presented. The aroma profile was analysed by headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC/MS), whereas the crude extract constituents were analyzed by 1 H-NMR and by high performance liquid chromatography coupled with mass spectrometry (HPLC/MS n ). Thirty-nine volatile compounds, most of them being monoterpenes, have been identified, with camphor, camphene, and α-pinene as the most abundant constituents. 1 H-NMR analysis revealed the presence of phenolic compounds and betulinic acid while HPLC/MS n allowed the identification of glycosilated and aglyconic flavonoids as well as phenylpropanoid derivatives. Some of these constituents, namely as betulinic acid, rosmanol, and cirsimaritin were reported for the first time in R. eriocalyx. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

Lipid nanocapsules containing the non-ionic surfactant Solutol HS15 inhibit the transport of calcium through hyperforin-activated channels in neuronal cells.

Science.gov (United States)

Chauvet, Sylvain; Barras, Alexandre; Boukherroub, Rabah; Bouron, Alexandre

2015-12-01

Hyperforin is described as a natural antidepressant inhibiting the reuptake of neurotransmitters and also activating cation channels. However the blood-brain barrier limits the access to the brain of this biomolecule. To circumvent this problem it was envisaged to encapsulate hyperforin into biomimetic lipid nano-carriers like lipid nanocapsules (LNCs). When testing the safety of 25 nm LNCs it appeared that they strongly blocked hyperforin-activated Ca2+ channels of cultured cortical neurons. This inhibition was due to one of their main component: solutol HS15 (polyoxyethylene-660-12-hydroxy stearate), a non-ionic soluble surfactant. Solutol HS15 rapidly depresses in a concentration-dependent manner the entry of Ca2+ through hyperforin-activated channels without influencing store-operated channels. This effect is mimicked by Brij58 but not by PEG600, indicating that the lipid chain of Solutol HS15 is important in determining its effects on the channels. The inhibition of the Ca2+ fluxes depends on the cellular cholesterol content; it is stronger after depleting cholesterol with methyl-β-cyclodextrin and is nearly absent on cells cultured in a cholesterol-rich medium. When chronically applied for 24 h, Solutol HS15 slightly up-regulates the entry of Ca2+ through hyperforin-activated channels. Similar observations were made when testing 25 nm lipid nanocapsules containing the surfactant Solutol HS15. Altogether, this study shows that Solutol HS15 perturbs in a cholesterol-dependent manner the activity of some neuronal channels. This is the first demonstration that LNCs containing this surfactant can influence cellular calcium signaling in the brain, a finding that can have important clinical implications. Copyright © 2015 Elsevier Ltd. All rights reserved.

Utilizing IXP1200 hardware and software for packet filtering

OpenAIRE

Lindholm, Jeffery L.

2004-01-01

As network processors have advanced in speed and efficiency they have become more and more complex in both hardware and software configurations. Intel's IXP1200 is one of these new network processors that has been given to different universities worldwide to conduct research on. The goal of this thesis is to take the first step in starting that research by providing a stable system that can provide a reliable platform for further research. This thesis introduces the fundamental hardware of In...

Clinical significance of determination of changes of serum hs-CRP, TNF-α and E2 levels in female children with simple obesity

International Nuclear Information System (INIS)

Xue Jie; Xu Weiqin; Zhou Hong; Liu Hongshu

2008-01-01

Objective: To explore the clinical significance of changes of serum hs-CRP, TNF-α and E 2 levels in female children with simple obesity. Methods: Serum levels of TNF-α, E 2 (with RIA) and hs-CRP (with immunity turbidity) were detected in 34 female children with simple obesity and 35 controls. Results: In the children with simple obesity, the serum hs-CRP, TNF- α and E 2 levels were significantly higher than those in controls (P 2 levels were mutually positively correlated (r=0.6182, 0.5784, P 2 levels is of help for assessment of severity of obesity as well as outcome prediction in female children. (authors)

Occupational Accident Declaration Form (HS50)

CERN Multimedia

HR Department

2007-01-01

https://cern.ch/service-procedures/AdminMan/Forms/HS50E.doc •\tIt must be completed within 10 working days of the date on which the accident occurred (§ 29.2.1), unless the person concerned is materially unable to meet this deadline. • The completed formula must be accompanied by a medical certificate giving details of any bodily injuries resulting from the accident (Annex 1, § 5). The medical certificate must be obtained from the doctor who has been consulted for that purpose. Benefits resulting from illnesses and accidents Medical treatment will cease to be reimbursed under the occupational scheme in the event of cure (defined in § 15 as the certified end to the impairment of the patient’s state of health caused by the illness or accident), or consolidation (defined in § 14 as certification that no further improvement in the patient’s state of health can be expected from appropriate medical treatment). The right to such reimbursement shall resume only in the...

Proinsulin, adiponectin and hsCRP in reproductive age women with polycystic ovary syndrome (PCOS)--the effect of metformin treatment.

Science.gov (United States)

Kruszyńska, Aleksandra; Słowińska-Srzednicka, Jadwiga; Jeske, Wojciech; Zgliczyński, Wojciech

2014-01-01

Women with polycystic ovary syndrome (PCOS) often suffer from obesity and insulin resistance. The role of proinsulin, which is known to be an indicator of fertility outcomes in PCOS women, and that of adiponectin, in the pathogenesis of PCOS is not well elucidated. Our objective was to determine proinsulin, adiponectin, hsCRP and other hormonal and metabolic parameters in PCOS women before and after metformin treatment. Two PCOS groups of patients of reproductive age (90 lean and 88 obese or overweight) with two control groups, adjusted for body mass index (BMI), were compared at baseline. 32 PCOS women were studied at baseline, after three and six months of metformin (1,000 mg/day) treatment. Clinical, anthropometric, biochemical and hormonal parameters were assessed. Proinsulin and hsCRP levels were the highest in obese PCOS women and were statistically different than in lean PCOS women (proinsulin: 11.4 v. 6.9 pmol/L; hsCRP 2.46 v. 0.47 mg/L, p treatment only in obese PCOS women. PCOS, when accompanied by obesity, is associated with elevated proinsulin concentrations, which correlates with higher hsCRP and increased FAI. Proinsulin level decreases due to metformin treatment. Our results suggest that obese or overweight PCOS and lean PCOS are characterised by different hormonal and metabolic parameters and have a different response to metformin treatment.

Investigating the Effect of Inflammation on Atrial Fibrillation Occurrence by Measuring Highly Sensitive C-reactive Protein (hs-CRP

Directory of Open Access Journals (Sweden)

Mahdi Hassanzadeh Delui

2014-05-01

Full Text Available Introduction: Atrial fibrillation (AF is the most prevalent cardiac arrhythmias that cardiologists and internists encounter. The goal of this article is to clarify an overview of the evidence linking inflammation to AF existence, which may highlight the effect of some pharmacological agents that have genuine potential to reduce the clinical burden of AF by modulating inflammatory pathways. Materials and Methods: In a case-control study, 50 patients with atrial fibrillation (AF with different etiologies and 50 patients with sinus rhythm and similar bases were selected. Sampling for highly sensitive c-reactive (hs-CRP was done on the patients presenting with AF to the Ghaem hospital between October 2006 and June 2007. Results: Mean age of the patients was 62 years with maximum of 90 and minimum of 36 and standard deviation of 13.80. The most frequent age group was 71-80years. Fifty-four percent of patients were male and 46% were female. Mean serum hs-CRP levels in AF patients with hypertension (HTN ,Ischemic heart disease(IHD, Valvular heart disease (VHD, HTN+IHD and hyperthyroidism were 8.10, 9.40, 8.68, 10.16 and 5.98 mg/Lit; respectively. There was significant difference between hs-CRP levels in hypertensive patients in the two groups (P=0.010. Similar results were observed in IHD patients, VHD patients and HTN+IHD patients in two groups (P=0.015, P=0.037, P=0.000. Conclusion: In addition to some risk factors like baseline cardiac diseases, aging, thyrotoxicosis, pulmonary embolism, pneumonia and cardiac surgery, there also appears to be consistent links between hs-CRP, a marker of inflammation, and the pathogenesis of AF. Introduction: Atrial fibrillation (AF is the most prevalent cardiac arrhythmias that cardiologists and internists encounter. The goal of this article is to clarify an overview of the evidence linking inflammation to AF existence, which may highlight the effect of some pharmacological agents that have genuine potential to

77 FR 19033 - Effect of Adding References to HS 6104.32 To Correct the U.S.-Korea FTA Product-Specific Rules of...

Science.gov (United States)

2012-03-29

... INTERNATIONAL TRADE COMMISSION [Investigation No. Korea FTA-103-026] Effect of Adding References to HS 6104.32 To Correct the U.S.- Korea FTA Product-Specific Rules of Origin AGENCY: United States... investigation No. Korea FTA-103-026, Effect of Adding References to HS 6104.32 To Correct the U.S.-Korea FTA...

Clinical significance of determination of changes in serum hs-CRP, IL-6, IL-10, and IL-18 levels after treatment in patients with acute conjunctivitis

International Nuclear Information System (INIS)

Liu Jun

2007-01-01

Objective: To study the clinical significance of changes of serum hs-CRP, IL-6, IL-10 and IL-18 levels in patients with acute conjunctivitis after treatment. Methods: Serum IL-6, IL-10 (with RIA) hs-CRP (with Immuno-turbidity) and IL-18 (with ELISA) levels were measured in 38 patients with acute conjunctivitis both before and after treatment as well as in 35 controls. Results: The serum hs-CRP, IL-6, IL-10 and IL-18 levels in the patients before treatment were significantly higher than those in the controls (P 0.05). Conclusion: Measurement of the changes of serum hs-CRP, IL-6, IL-10 and IL-18 levels after treatment might be inportant for outcome prediction in patients with acute conjunctivitis. (authors)

SugE belongs to the small multidrug resistance (SMR) protein family involved in tributyltin (TBT) biodegradation and bioremediation by alkaliphilic Stenotrophomonas chelatiphaga HS2.

Science.gov (United States)

Hassan, Hamdy A

2018-03-01

Tributyltin (TBT) used in a variety of industrial processes, subsequent discharge into the environment, its fate, toxicity and human exposure are topics of current concern. TBT degradation by alkaliphilic bacteria may be a key factor in the remediation of TBT in high pH contaminated sites. In this study, Stenotrophomonas chelatiphaga HS2 were isolated and identified from TBT contaminated site in Mediterranean Sea. S. chelatiphaga HS2 has vigor capability to transform TBT into dibutyltin and monobutyltin (DBT and MBT) at pH 9 and 7% NaCl (w/v). A gene was amplified and characterized from strain HS2 as SugE protein belongs to SMR protein family, a reverse transcription polymerase chain reaction analysis confirmed that SugE protein involved in the TBT degradation by HS2 strain. TBT bioremediation was investigated in stimulated TBT contaminated sediment samples (pH 9) using S chelatiphaga HS2 in association with E. coli BL21 (DE3)-pET28a(+)-sugE instead of S chelatiphaga HS2 alone reduced significantly the TBT half-life from 12d to 5d, although no TBT degradation appeared using E. coli BL21 (DE3)-pET28a(+)-sugE alone. This finding indicated that SugE gene increased the rate and degraded amount of TBT and is necessary in enhancing TBT bioremediation. Copyright © 2017 Elsevier B.V. All rights reserved.

The effect of novel surfactants and Solutol HS 15 on paclitaxel aqueous solubility and permeability across a Caco-2 monolayer.

Science.gov (United States)

Alani, Adam W G; Rao, Deepa A; Seidel, Ron; Wang, Jian; Jiao, Jim; Kwon, Glen S

2010-08-01

The effect of novel surfactants on the aqueous solubility and the permeability of paclitaxel across a Caco-2 cell monolayer were examined in this work. The solubility and permeability of paclitaxel was evaluated in the presence of four soft surfactants (SS) KXN441, KXN424, KXN437, and KXN 337 and Solutol HS15. All surfactants increased the aqueous solubility of paclitaxel. Caco-2 cell membrane integrity in the presence of SS and Solutol HS15 was assessed by mannitol permeability and LDH release. All surfactants were tested at 0.5x CMC, 5x CMC and 1.5 mM concentrations. The effect of SSs on paclitaxel permeability was concentration dependent. At all concentrations tested, KXN 441 and Solutol HS 15 showed partially inhibition of drug efflux with no discernable change in mannitol permeability or cytotoxicity as observed with LDH release. At these concentrations, other SSs exhibited some partial efflux inhibition along with compromised membrane integrity and increasing mannitol permeability. In conclusion, all SSs were able to increase the aqueous solubility and permeability of paclitaxel across Caco-2 cells monolayer. However, KXN441 and Solutol HS15 were able to enhance paclitaxel permeability across Caco-2 monolayer without cytotoxicity. (c) 2010 Wiley-Liss, Inc. and the American Pharmacists Association

Essential oil from Rhaponticum acaule L. roots: Comparative study using HS-SPME/GC/GC–MS and hydrodistillation techniques

Directory of Open Access Journals (Sweden)

Batoul Benyelles

2014-12-01

Full Text Available The composition of essential oil extracted from Rhaponticum acaule L. roots growing wild in Algeria was studied by hydrodistillation (HD and by Head-Space Solid Phase Micro-Extraction (HS-SPME. Quantitative but not qualitative differences have been found in the chemical composition of both analysed samples depending on the extraction method. However, the oil obtained from R. acaule roots shows that aliphatic alcohols were found to be the major class (69.2%, followed by the terpenes (5.5%, alkenes (5.2% and alkynes (4.0%. In both cases the analysis were carried out using Gas Chromatography (GC and Gas Chromatography–Mass Spectrometry (GC–MS. Our study shows that HS-SPME extraction could be considered as an alternative technique for the isolation of volatiles from plant. 25 components were identified in oil vs. 39 in the HS-SPME. However the oil composition of roots was mainly represented by a variety of aliphatic hydrocarbons (alcohols, aldehydes and ketones and terpenes which are known for their antimicrobial activities.

ExoMol line lists - XXIX. The rotation-vibration spectrum of methyl chloride up to 1200 K

Science.gov (United States)

Owens, A.; Yachmenev, A.; Thiel, W.; Fateev, A.; Tennyson, J.; Yurchenko, S. N.

2018-06-01

Comprehensive rotation-vibration line lists are presented for the two main isotopologues of methyl chloride, 12CH335Cl and 12CH337Cl. The line lists, OYT-35 and OYT-37, are suitable for temperatures up to T = 1200 K and consider transitions with rotational excitation up to J = 85 in the wavenumber range 0-6400 cm-1 (wavelengths λ > 1.56 μm). Over 166 billion transitions between 10.2 million energy levels have been calculated variationally for each line list using a new empirically refined potential energy surface, determined by refining to 739 experimentally derived energy levels up to J = 5, and an established ab initio dipole moment surface. The OYT line lists show excellent agreement with newly measured high-temperature infrared absorption cross-sections, reproducing both strong and weak intensity features across the spectrum. The line lists are available from the ExoMol database and the CDS database.

Optimization of HS-SPME/GC-MS analysis and its use in the profiling of illicit ecstasy tablets (Part 1).

Science.gov (United States)

Bonadio, Federica; Margot, Pierre; Delémont, Olivier; Esseiva, Pierre

2009-05-30

A headspace solid-phase microextraction procedure (HS-SPME) was developed for the profiling of traces present in 3,4-methylenedioxymethylampethamine (MDMA). Traces were first extracted using HS-SPME and then analyzed by gas chromatography-mass spectroscopy (GC-MS). The HS-SPME conditions were optimized using varying conditions. Optimal results were obtained when 40 mg of crushed MDMA sample was heated at 80 degrees C for 15 min, followed by extraction at 80 degrees C for 15 min with a polydimethylsiloxane/divinylbenzene coated fibre. A total of 31 compounds were identified as traces related to MDMA synthesis, namely precursors, intermediates or by-products. In addition some fatty acids used as tabletting materials and caffeine used as adulterant, were also detected. The use of a restricted set of 10 target compounds was also proposed for developing a screening tool for clustering samples having close profile. 114 seizures were analyzed using an SPME auto-sampler (MultiPurpose Samples MPS2), purchased from Gerstel GMBH & Co. (Germany), and coupled to GC-MS. The data was handled using various pre-treatment methods, followed by the study of similarities between sample pairs based on the Pearson correlation. The results show that HS-SPME, coupled with the suitable statistical method is a powerful tool for distinguishing specimens coming from the same seizure and specimens coming from different seizures. This information can be used by law enforcement personnel to visualize the ecstasy distribution network as well as the clandestine tablet manufacturing.

Improved selectivity and cytotoxic effects of irinotecan via liposomal delivery: A comparative study on Hs68 and HeLa cells.

Science.gov (United States)

Casadó, Ana; Mora, Margarita; Sagristá, Maria Lluïsa; Rello-Varona, Santi; Acedo, Pilar; Stockert, Juan Carlos; Cañete, Magdalena; Villanueva, Angeles

2017-11-15

Irinotecan (CPT-11) is an effective chemotherapeutic agent widely used to treat different cancers. Otherwise, the liposomal delivery of anti-tumor agents has been shown to be a promising strategy. The aim of this study has been to analyze the effect of liposomal CPT-11 (CPT-11lip) on two human cell lines (Hs68 and HeLa) to establish the suitability of this CPT-11 nanocarrier. We have demonstrated the highest uptake of CPT-11lip in comparison with that of CPT-11sol, in lactate buffer, and that CPT-11lip was internalized in the cells through an endocytic process whereas CPT-11sol does so by passive diffusion. CPT-11lip was not cytotoxic to normal fibroblast Hs68 cells, but induced a massive apoptosis accompanied by cell senescence in HeLa cells. CPT-11lip treatment modified the morphology of HeLa cells, induced different cell cycle alterations and accumulated into lysosomes in both cell lines. In particular, CPT-11lip treatment showed that surviving HeLa cells remained in a state of senescence whereas only a temporal growth arrest was induced in Hs68 cells. Results of RT-PCR indicated that the different responses in Hs68 (survival) and HeLa cells (apoptotic death), seemed to be induced by a p53- and p53- independent mechanism, respectively. An analysis of DNA damage also determined that released CPT-11 from liposomes was able to reach the nucleus and exert a genotoxic effect in both cell lines, which was repaired in Hs68 but not in HeLa cells. All results indicate that phospholipid-cholesterol liposomes possess optimum properties for CPT-11 delivery, being biocompatible and selectively cytotoxic against HeLa tumorigenic cells. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Analysis of the optical emission of the young precataclysmic variables HS 1857+5144 and ABELL 65

Science.gov (United States)

Shimansky, V. V.; Pozdnyakova, S. A.; Borisov, N. V.; Bikmaev, I. F.; Vlasyuk, V. V.; Spiridonova, O. I.; Galeev, A. I.; Mel'Nikov, S. S.

2009-10-01

We analyze the physical state and the properties of the close binary systems HS 1857+5144 and Abell 65. We took the spectra of both systems over a wide range of orbital phases with the 6-m telescope of the Special Astrophysical Observatory of the Russian Academy of Sciences (SAO RAS) and obtained their multicolor light curves with the RTT150 and Zeiss-1000 telescopes of the SAO RAS. We demonstrate that both Abell 65 and HS 1857+5144 are young precataclysmic variables (PV) with orbital periods of P orb = 1. d 003729 and P orb = 0. d 26633331, respectively. The observed brightness and spectral variations during the orbital period are due to the radiation of the cold component, which absorbs the short-wave radiation of the hot component and reemits it in the visual part of the spectrum. A joint analysis of the brightness and radial velocity curves allowed us to find the possible and optimum sets of their fundamental parameters. We found the luminosity excesses of the secondary components of HS 1857+5144 and Abell 65 with respect to the corresponding Main Sequence stars to be typical for such objects. The excess luminosities of the secondary components of all young PVs are indicative of their faster relaxation rate towards the quiescent state compared to the rates estimated in earlier studies.

Restriction of the use of hazardous substances (RoHS in the personal computer segment: analysis of the strategic adoption by the manufacturers settled in Brazil

Directory of Open Access Journals (Sweden)

Ademir Brescansin

2015-09-01

Full Text Available The enactment of the RoHS Directive (Restriction of Hazardous Substances in 2003, limiting the use of certain hazardous substances in electronic equipment has forced companies to adjust their products to comply with this legislation. Even in the absence of similar legislation in Brazil, manufacturers of personal computers which are located in this country have been seen to adopt RoHS for products sold in the domestic market and abroad. The purpose of this study is to analyze whether these manufacturers have really adopted RoHS, focusing on their motivations, concerns, and benefits. This is an exploratory study based on literature review and interviews with HP, Dell, Sony, Lenovo, Samsung, LG, Itautec, and Positivo, using summative content analysis. The results showed that initially, global companies adopted RoHS to market products in Europe, and later expanded this practice to all products. Brazilian companies, however, adopted RoHS to participate in the government’s sustainable procurement bidding processes. It is expected that this study can assist manufacturers in developing strategies for reducing or eliminating hazardous substances in their products and processes, as well as help the government to formulate public policies on reducing risks of environmental contamination.

Innovative analysis of HS 6-5-2 drills edges quality

Directory of Open Access Journals (Sweden)

Daicu Raluca

2017-01-01

Full Text Available The paper is analyzing the quality of Ø8 drills edges from HS 6-5-2 steel, using an innovative technique, the measurement of electrical current at cutting. The method is useful to take the right decision about drills acquisition from different suppliers based on the quality/price ratio. Also, it can be made a fast quality comparison of drills that leads to a selection of the acquisition places. The analysis of a drill batch is done using the microscope and the measurement of the electrical current at cutting, showing the better accuracy of the last method.

High luminescent fibers with hybrid SiO2-coated CdTe nanocrystals fabricated by electrospinning technique

International Nuclear Information System (INIS)

Cao, Yongqiang; Liu, Ning; Yang, Ping; Shi, Ruixia; Ma, Qian; Zhang, Aiyu; Zhu, Yuanna; Wang, Junpeng; Wang, Jianrong

2015-01-01

The polyvinylpyrrolidone (PVP) hybrid luminescent micro-/nanofibers doped with the novel hybrid SiO 2 -coated CdTe nanocrystals (HS-CdTe NCs) have been fabricated for the first time via the electrospinning technique. The morphologies and photoluminescence (PL) emissions of HS-CdTe/PVP micro-/nanofibers prepared by doping the HS-CdTe NCs with the different PL peak wavelength (571, 616, and 643 nm) in PVP fibers were investigated by optical and PL microscope. The results revealed that all the HS-CdTe/PVP hybrid fibers showed an ultralong length for several hundreds of micrometers and a relatively uniform diameter of 1000 ∼ 1200 nm. The hybrid fibers displayed a wavelength-tunable PL emission, determining by the PL of doped HS-CdTe NCs. Moreover, similar to the original PL properties of HS-CdTe NCs before the electrospinning, the HS-CdTe/PVP fibers also showed a series of superior PL properties, such as narrow and symmetry PL spectrum, high, and uniform brightness. For comparison purpose, we also prepared three CdTe/PVP hybrid fibers by doping the 553 nm, 600 nm, and 633 nm PL-emitting CdTe NCs respectively in PVP electrospinning fibers. The characterization results showed that, the obtained three CdTe/PVP hybrid fibers had a basically satisfactory micro-/nanofiber morphology with a long length and relatively uniform diameter, but all the fibers exhibited very weak PL emissions. The enormous contrast in PL properties between HS-CdTe/PVP and CdTe/PVP fibers should mainly be ascribed to the different connection modes of ligands with the NCs and the passivation effect of inert hybrid silica shell on HS-CdTe. It is hopeful that the high luminescent HS-CdTe/PVP micro-/nanofibers with the tunable PL peak wavelength would be a good candidate in the optical sensor, light-emitting devices (LEDs), nanometer-scale waveguides, and the other related photonic materials. - Highlights: • The HS-CdTe/PVP electrospun hybrid fibers were fabricated for the first time. • The

Data management design: nucleon-nucleon data bank (0 to 1200 MeV)

International Nuclear Information System (INIS)

Signell, P.; Freiheit, F.

1981-01-01

This is a guide to the design of the on-line data records in this bank, covering all energies and particle combinations up to about 1200 MeV. The design of the bank's management system satisfies almost all of the conditions set forth in the proposed Design Principles for Physics Data Banks

Investigating the Effect of Inflammation on Atrial Fibrillation Occurrence by Measuring Highly Sensitive C-reactive Protein (hs-CRP

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Mahdi Hassanzadeh Delui

2014-05-01

Full Text Available Introduction: Atrial fibrillation (AF is the most prevalent cardiac arrhythmias that cardiologists and internists encounter. The goal of this article is to clarify an overview of the evidence linking inflammation to AF existence, which may highlight the effect of some pharmacological agents that have genuine potential to reduce the clinical burden of AF by modulating inflammatory pathways. Materials and Methods: In a case-control study, 50 patients with atrial fibrillation (AF with different etiologies and 50 patients with sinus rhythm and similar bases were selected. Sampling for highly sensitive c-reactive (hs-CRP was done on the patients presenting with AF to the Ghaem hospital between October 2006 and June 2007. Results: Mean age of the patients was 62 years with maximum of 90 and minimum of 36 and standard deviation of 13.80. The most frequent age group was 71-80years. Fifty-four percent of patients were male and 46% were female. Mean serum hs-CRP levels in AF patients with hypertension (HTN ,Ischemic heart disease(IHD, Valvular heart disease (VHD, HTN+IHD and hyperthyroidism were 8.10, 9.40, 8.68, 10.16 and 5.98 mg/Lit; respectively. There was significant difference between hs-CRP levels in hypertensive patients in the two groups (P=0.010. Similar results were observed in IHD patients, VHD patients and HTN+IHD patients in two groups (P=0.015, P=0.037, P=0.000. Conclusion: In addition to some risk factors like baseline cardiac diseases, aging, thyrotoxicosis, pulmonary embolism, pneumonia and cardiac surgery, there also appears to be consistent links between hs-CRP, a marker of inflammation, and the pathogenesis of AF.

A comparison study on a sulfonated graphene-polyaniline nanocomposite coated fiber for analysis of nicotine in solid samples through the traditional and vacuum-assisted HS-SPME.

Science.gov (United States)

Ghiasvand, Alireza; Koonani, Samira; Yazdankhah, Fatemeh; Farhadi, Saeid

2018-02-05

A simple, rapid, and reliable headspace solid-phase microextraction (HS-SPME) procedure, reinforced by applying vacuum in the extraction vial, was developed. It was applied for the extraction of nicotine in solid samples prior to determination by gas chromatography-flame ionization detection (GC-FID). First, the surface of a narrow stainless steel wire was made porous and adhesive by platinization to obtain a durable, higher surface area, and resistant fiber. Then, a thin film of sulfonated graphene/polyaniline (Sulf-G/PANI) nanocomposite was synthesized and simultaneously coated on the platinized fiber using the electrophoretic deposition (EPD) method. It was demonstrated that the extraction efficiency remarkably increased by applying the reduced-pressure condition in the extraction vial. To evaluate the conventional HS-SPME and vacuum-assisted HS-SPME (VA-HS-SPME) platforms, all experimental parameters affecting the extraction efficiency including desorption time and temperature, extraction time and temperature and moisture content of sample matrix were optimized. The highest extraction efficiency was obtained at 60°C, 10min (extraction temperature and time) and 280°C, 2min (desorption condition), for VA-HS-SPME strategy, while for conventional HS-SPME the extraction and desorption conditions found to be 100°C, 30min and 280°C, 2min, respectively. The Sulf-G/PANI coated fiber showed high thermal stability, good chemical/mechanical resistance, and long lifetime. For analysis of nicotine in solid samples using VA-HS-SPME-GC-FID, linear dynamic range (LDR) was 0.01-30μgg -1 (R 2 =0.996), the relative standard deviation (RSD%, n=6), for analyses of 1μgg -1 nicotine was calculated 3.4% and limit of detection (LOD) found to be 0.002μgg -1 . The VA-HS-SPME-GC-FID strategy was successfully carried out for quantitation of nicotine in hair and tobacco real samples. Copyright © 2017 Elsevier B.V. All rights reserved.

The human homolog of S. cerevisiae CDC27, CDC27 Hs, is encoded by a highly conserved intronless gene present in multiple copies in the human genome

Energy Technology Data Exchange (ETDEWEB)

Devor, E.J.; Dill-Devor, R.M. [Univ. of Iowa College of Medicine, Iowa City (United States)

1994-09-01

We have obtained a number of unique sequences via PCR amplification of human genomic DNA using degenerate primers under low stringency (42{degrees}C). One of these, an 853 bp product, has been identified as a partial genomic sequence of the human homolog of the S. cerevisiae CDC27 gene, CDC27Hs (GenBank No. U00001). This gene, reported by Turgendreich et al. is also designated EST00556 from Adams et al. We have undertaken a more detailed examination of our sequence, MCP34N, and have found that: 1. the genomic sequence is nearly identical to CDC27Hs over its entire 853 bp length; 2. an MCP34N-specific PCR assay of several non-human primate species reveals amplification products in chimpanzee and gorilla genomes having greater than 90% sequence identity with CDC27Hs; and 3. an MCP34N-specific PCR assay of the BIOS hybrid cell line panel gives a discordancy pattern suggesting multiple loci. Based upon these data, we present the following initial characterization: 1. the complete MCP34N sequence identity with CDC27Hs indicates that the latter is encoded by an intronless gene; 2. CDC27Hs is highly conserved among higher primates; and 3. CDC27Hs is present in multiple copies in the human genome. These characteristics, taken together with those initially reported for CDC27Hs, suggest that this is an old gene that carries out an important but, as yet, unknown function in the human brain.

Correlation between Interleukin-6 (IL-6, High Sensitivity C-Reactive Protein (hsCRP, Endothelin-1 (ET-1, Asymmetric Dimethylarginine (ADMA and Insulin Resistance (HOMA-IR in Central Obese Men

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Andri Hidayat

2011-04-01

Full Text Available BACKGROUND: Many studies have shown that obesity was closely related to insulin resistance via several pathways such as inflammation, oxidative stress, lipolysis, and endothelial dysfunction. This study was carried out to observe the correlation between inflammation (IL-6 and hsCRP, lipolysis process (ET-1, and endothelial dysfunction (ADMA and insulin resistance (HOMA-IR in centrally obese men. METHODS: This was a cross sectional study on 62 male subjects aged 30–60 years old with waist circumference (WC >90 cm. IL-6, ET-1 and ADMA levels were measured using ELISA method, while hsCRP and insulin were measured using chemiluminescence method. All blood testings were conducted in Prodia Clinical Laboratory. RESULTS: The results showed that WC was significantly correlated with hsCRP (r=0.294, p=0.022, ET-1 (r=0.257, p=0.047 and ADMA (r=0.338, p=0.009. We also found a significant correlation between hsCRP with HOMA-IR (r=0.324, p=0.021, ADMA with HOMA-IR (r=0.280, p=0.045 and IL-6 with hsCRP (r=0.437, p=0.003. CONCLUSIONS: hsCRP and ADMA have significant correlation with HOMA-IR in centrally obese men. HOMA-IR significantly increases in subjects with ADMA above median and either IL-6 or hsCRP above median, as compared to those in the other groups. Inflammation and endothelial dysfunction are important causal pathways of insulin resistance state in centrally obese men. KEYWORDS: obesity, IL-6, hsCRP, ET-1, ADMA, HOMA-IR.

HURRICANE AND SEVERE STORM SENTINEL (HS3) GLOBAL HAWK HIGH ALTITUDE MMIC SOUNDING RADIOMETER (HAMSR) V1

Data.gov (United States)

National Aeronautics and Space Administration — The Hurricane and Severe Storm Sentinel (HS3) Global Hawk High Altitude MMIC Sounding Radiometer (HAMSR) datasets include measurements gathered by the HAMSR...

HIGH-SENSITIVITY C-REACTIVE PROTEIN (hsCRP IN YOUNG ADULTS: RELATION TO AEROBIC CAPACITY, PHYSICAL ACTIVITY AND RISK FACTORS FOR CARDIOVASCULAR DISEASES

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K. Mazurek

2011-11-01

Full Text Available Atheromatosis develops as a result of a chronic inflammatory process of the arteries. Inflammatory biomarkers, particularly high-sensitivity C-reactive protein (hsCRP, positively correlate with atheromatosis risk factors and can be used to estimate and predict the risk of cardiovascular events. The purpose of this study was to evaluate the relationship between hsCRP concentration and BMI, body composition, classical risk factors for cardiovascular diseases, energy expenditure for physical activity (WEE and  ·VO2max. 166 volunteers (78 women and 88 men were included in the examinations. Their mean age was 20.2±0.9 years. Health condition was described by the following variables: smoking, WEE,  ·VO2max, body mass index (BMI, waist-to-hip ratio (WHR, fat mass (FM, fat-free mass (FFM, lipid profile, hsCRP, glucose and insulin concentration, and insulin resistance. Between the subgroups created on the basis of hsCRP concentration, in quartiles 1 to 3 and quartile 4, a comparative analysis was carried out. 79.5�0of women and 69.3�0of men had hsCRP values within the references ranges. Moderately high values were found in 14.1�0of women and 22.7�0of men and high in 6.4�0and 7.9�20respectively. Mean values of BMI, FFM, WHR, WEE,  ·VO2max, glucose and triglyceride concentration, and TC/HDL index were significantly lower, while FM and HDL were significantly higher, in women than in men. In the quartile 4 subgroup compared to the quartile 1-3 subgroup, we found significantly lower HDL concentration and a tendency for higher values of BMI (p=0.06 and TC (p=0.07 as well as higher percentages of smoking among men. In young, physically active, healthy persons, serum concentration of hsCRP is not related to physical activity or  ·VO2max.

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China.

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Junfeng Liu

Full Text Available Elevated levels of high sensitivity cardiac troponin T (hs-cTnT occur in a substantial proportion of patients with acute ischemic stroke (AIS and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT or prognosis in AIS patients with rheumatic heart disease (RHD remains unclear.Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within 1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels (≥14 ng/L and patients with normal hs-cTnT levels (<14 ng/L.The final analysis involved 84 patients (31 males; mean age, 61.6±12.2 years, of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028, and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029. After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels.Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies.

Effects of RoHs and REACH regulations on firm-level production and export, and the role of global value chains : the cases of Malaysia and Vietnam

OpenAIRE

Otsuki, Tsunehiro; Honda, Keiichiro; Michida, Etsuyo; Nabeshima, Kaoru; Ueki, Yasushi

2015-01-01

This paper uses firm-level data to examine the impact of foreign chemical safety regulations such as RoHS and REACH on the production costs and export performance of firms in Malaysia and Vietnam. This paper also investigates the role of global value chains in enhancing the likelihood that a firm complies with RoHS and REACH. We find that in addition to the initial setup costs for compliance, EU RoHS (REACH) implementation imposes on firms additional variable production costs by requiring add...

Development of a HS-SPME-GC/MS protocol assisted by chemometric tools to study herbivore-induced volatiles in Myrcia splendens.

Science.gov (United States)

Souza Silva, Érica A; Saboia, Giovanni; Jorge, Nina C; Hoffmann, Camila; Dos Santos Isaias, Rosy Mary; Soares, Geraldo L G; Zini, Claudia A

2017-12-01

A headspace solid phase microextraction (HS-SPME) method combined with gas chromatography-mass spectrometry (GC/MS) was developed and optimized for extraction and analysis of volatile organic compounds (VOC) of leaves and galls of Myrcia splendens. Through a process of optimization of main factors affecting HS-SPME efficiency, the coating divivnilbenzene-carboxen-polydimethylsiloxane (DVB/Car/PDMS) was chosen as the optimum extraction phase, not only in terms of extraction efficiency, but also for its broader analyte coverage. Optimum extraction temperature was 30°C, while an extraction time of 15min provided the best compromise between extraction efficiencies of lower and higher molecular weight compounds. The optimized protocol was demonstrated to be capable of sampling plant material with high reproducibility, considering that most classes of analytes met the 20% RSD FDA criterion. The optimized method was employed for the analysis of three classes of M. splendens samples, generating a final list of 65 tentatively identified VOC, including alcohols, aldehydes, esters, ketones, phenol derivatives, as well as mono and sesquiterpenes. Significant differences were evident amongst the volatile profiles obtained from non-galled leaves (NGL) and leaf-folding galls (LFG) of M. splendens. Several differences pertaining to amounts of alcohols and aldehydes were detected between samples, particularly regarding quantities of green leaf volatiles (GLV). Alcohols represented about 14% of compounds detected in gall samples, whereas in non-galled samples, alcohol content was below 5%. Phenolic derived compounds were virtually absent in reference samples, while in non-galled leaves and galls their content ranged around 0.2% and 0.4%, respectively. Likewise, methyl salicylate, a well-known signal of plant distress, amounted for 1.2% of the sample content of galls, whereas it was only present in trace levels in reference samples. Chemometric analysis based on Heatmap associated

Association between CD8 T-cell subsets and CD4/CD8 ratio with HS-CRP level in HIV-infected patients on antiretroviral therapy

Science.gov (United States)

Isabela, S.; Nugroho, A.; Harijanto, P. N.

2018-03-01

Due to improved access and adherence to antiretroviral therapy (ART), most HIV-infected persons worldwide are predicted to live longer. Nowadays the cause of death for most HIV-infected persons has changed to serious non-AIDS events (SNAEs) which is due to low-grade viremia. HIV patients with ART who had undergone CD4 cell count above 500/uL and there is an increase in hs-CRP despite an undetectable viral load. Some conditions CD8 cells count do not decrease with CD4 cells repairs. We researched in Prof Kandou General Hospital with a total sample of 35 HIV patients who had received ART with the level of CD4>350/uL. CD8 levels, CD4/CD8 ratio, and hs-CRP were assessed. This research is analytic descriptive with cross-sectional study design and analysis uses Spearman correlation. The mean CD8 during the study was 1291.8 (IQR 319-2610cells/uL), the mean ratio of CD4:CD8 was 0.57 (IQR 0.16-1.24) and median hs-CRP is 2.18 (IQR 0.3-6.6mg/dL). There was a significant positive correlation between CD8 and increased hs-CRP (r=0.369, pCD4/CD8 ratio and hs-CRP (r=-0.370, p<0.05).

High-sensitivity C-reactive protein (hs-CRP) levels and its relationship with components of polycystic ovary syndrome in Indian adolescent women with polycystic ovary syndrome (PCOS).

Science.gov (United States)

Ganie, Mohd Ashraf; Hassan, Saqib; Nisar, Sobia; Shamas, Nasir; Rashid, Aafia; Ahmed, Ishfaq; Douhat, Syed; Mudassar, Syed; Jan, Vicar M; Rashid, Fouzia

2014-11-01

C-reactive protein (CRP) is a risk marker for type 2 diabetes mellitus and cardiovascular diseases. In polycystic ovary syndrome (PCOS), limited data are available on high-sensitivity C-reactive protein (hs-CRP) levels and its relationship with components of PCOS especially in Indian women. The objective was to determine serum hs-CRP concentration in adolescent women with and without PCOS and to assess possible correlations of serum hs-CRP levels with components of PCOS in Indian women. One hundred and sixty women with PCOS and sixty non-PCOS women having normal menstrual cycles were included. Clinical assessment included anthropometry, Ferriman-Gallwey (FG) score and blood pressure (BP) measurement. Laboratory evaluation included estimation of T4, TSH, LH, FSH, total testosterone, prolactin, cortisol, 17OHP, hs-CRP, lipid profile, and insulin, and glucose after 2-h oral glucose tolerance test. Homeostasis Model Assessment Insulin resistance index (HOMA-IR) and Quantitative Insulin Sensitivity Check Index (QUICKI) and glucose intolerance was calculated. FG score, LH, FSH, total Testosterone, HOMA-IR and QUICKI were significantly different among women with or without PCOS (p women having PCOS, there was no significant difference between the groups (p > 0.05). A significant and positive correlation was found between hs-CRP and body mass index (BMI) (r = 0.308, p Indian adolescent women suggest that hs-CRP levels may not per se be associated with PCOS, rather can be related to fat mass in this subset of subjects.

Laser surface alloying of aluminum (AA1200) with Ni and SiC Powders

CSIR Research Space (South Africa)

Mabhali, Luyolo AB

2010-12-01

Full Text Available . The dissociated C reacted with Al to form Al4C3. The addition of Ni resulted in the formation of the Al3Ni phase. A hardness increase of approximately four times that of aluminum AA1200 was achieved in the alloyed layer....

HS-SPME AS AN EFFICIENT TOOL FOR DISCRIMINATING CHEMOTYPES OF Lippia alba (Mill. N. E. Brown

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Aiêrta C. C. da Silva

Full Text Available Lippia alba (Mill. N. E. Brown (Verbenaceae is a medicinal plant for which several biological activities are reported, such as sedative, anxiolytic, anti-ulcer, antifungal, antimicrobial, antioxidant, antispasmodic, anti-nociceptive and anti-inflammatory. It is characterized by the production of essential oils which have been used to classify the plant in different chemotypes. In the Northeast region of Brazil, the presence of three chemotypes are reported: myrcene-citral (chemotype I, limonene-citral (chemotype II and carvone-limonene (chemotype III. In this work, headspace-solid phase microextraction (HS-SPME was used on the analysis of the volatile organic compounds (VOCs of three chemotypes of L. alba from the Northeast region of Brazil, and compared to the essential oils of the plants extracted by hydrodistillation. Volatile compounds from each chemotype were more effectively differentiated when extracted by HS-SPME than by hydrodistillation.

Nucleolin is a nuclear target of heparan sulfate derived from glypican-1

International Nuclear Information System (INIS)

Cheng, Fang; Belting, Mattias; Fransson, Lars-Åke; Mani, Katrin

2017-01-01

The recycling, S-nitrosylated heparan sulfate (HS) proteoglycan glypican-1 releases anhydromannose (anMan)-containing HS chains by a nitrosothiol-catalyzed cleavage in endosomes that can be constitutive or induced by ascorbate. The HS-anMan chains are then transported to the nucleus. A specific nuclear target for HS-anMan has not been identified. We have monitored endosome-to-nucleus trafficking of HS-anMan by deconvolution and confocal immunofluorescence microscopy using an anMan-specific monoclonal antibody in non-growing, ascorbate-treated, and growing, untreated, wild-type mouse embryonic fibroblasts and hypoxia-exposed Alzheimer mouse Tg2576 fibroblasts and human U87 glioblastoma cells. In all cells, nuclear HS-anMan targeted a limited number of sites of variable size where it colocalized with DNA and nucleolin, an established marker for nucleoli. HS-anMan also colocalized with ethynyl uridine-tagged nascent RNA and two acetylated forms of histone H3. Acute hypoxia increased the formation of HS-anMan in both Tg2576 and U87 cells. A portion of HS-anMan colocalized with nucleolin at small discrete sites, while most of the nucleolin and nascent RNA was dispersed. In U87 cells, HS-anMan, nucleolin and nascent RNA reassembled after prolonged hypoxia. Nucleolar HS may modulate synthesis and/or release of rRNA.

Nucleolin is a nuclear target of heparan sulfate derived from glypican-1

Energy Technology Data Exchange (ETDEWEB)

Cheng, Fang [Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Biomedical Center A13, SE-221 84 Lund (Sweden); Belting, Mattias [Department of Clinical Sciences, Section of Oncology and Pathology, Lund University, Lund (Sweden); Fransson, Lars-Åke [Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Biomedical Center A13, SE-221 84 Lund (Sweden); Mani, Katrin, E-mail: katrin.mani@med.lu.se [Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Biomedical Center A13, SE-221 84 Lund (Sweden)

2017-05-01

The recycling, S-nitrosylated heparan sulfate (HS) proteoglycan glypican-1 releases anhydromannose (anMan)-containing HS chains by a nitrosothiol-catalyzed cleavage in endosomes that can be constitutive or induced by ascorbate. The HS-anMan chains are then transported to the nucleus. A specific nuclear target for HS-anMan has not been identified. We have monitored endosome-to-nucleus trafficking of HS-anMan by deconvolution and confocal immunofluorescence microscopy using an anMan-specific monoclonal antibody in non-growing, ascorbate-treated, and growing, untreated, wild-type mouse embryonic fibroblasts and hypoxia-exposed Alzheimer mouse Tg2576 fibroblasts and human U87 glioblastoma cells. In all cells, nuclear HS-anMan targeted a limited number of sites of variable size where it colocalized with DNA and nucleolin, an established marker for nucleoli. HS-anMan also colocalized with ethynyl uridine-tagged nascent RNA and two acetylated forms of histone H3. Acute hypoxia increased the formation of HS-anMan in both Tg2576 and U87 cells. A portion of HS-anMan colocalized with nucleolin at small discrete sites, while most of the nucleolin and nascent RNA was dispersed. In U87 cells, HS-anMan, nucleolin and nascent RNA reassembled after prolonged hypoxia. Nucleolar HS may modulate synthesis and/or release of rRNA.

Macrophage inflammatory protein-3α influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells

International Nuclear Information System (INIS)

Lee, Y.C.; Chiou, T.-J.; Tzeng, W.-F.; Chu, S.T.

2008-01-01

Stromal cell monolayers have been an important means of studying the regulation of hematopoiesis, because they produce cytokines. Cytosine arabinoside, vincristine, daunorubicin, and doxorubicin are common drugs for hematological cancer therapy, and they may have some effects on bone marrow stroma during chemotherapy. The aim of this study was to elucidate interactions between the bone marrow stromal microenvironment and leukemic cells after drug treatment. We tested the hypothesis that human HS-5 stromal cells, pretreated with anticancer drugs, affected the growth of leukemic K562 cells by changing the cytokines in the culture microenvironment. Thereafter, proliferation of K562 cells increased nearly 2.5-fold compared the co-cultivation with drugs-pretreated HS-5 stromal cells and drugs-untreated HS-5 stromal cells. The results indicated that co-cultivation with HS-5 stromal cells pretreated with drugs caused significant K562 cell proliferation. Cytokines in the microenvironment were detected via the RayBio Human Cytokine Antibody Array Membrane. The levels of the cytokines CKβ, IL-12, IL-13, IGFBP-2, MCP-1, MCP-3, MCP-4, MDC, MIP-1β and MIP-1δ were decreased, with a particularly marked decrease in MIP-3α. In co-culture medium, there was a 20-fold decrease in MIP-3α in daunorubicin-pretreated HS-5 cells and at least a 3-fold decrease in Ara-C-pretreated cells. This indicated a significant effect of anticancer drugs on the stromal cell line. Using phosphorylated Erk and pRb proteins as cell proliferation markers, we found that phosphorylation of these markers in K562 cells was inhibited during co-cultivation with drug-pretreated stromal cells in MIP-3α-supplemented medium and restored by MIP-3α antibody supplement. In conclusion, anticancer drug pretreatment suppresses the negative control exerted by HS-5 cells on leukemic cell proliferation, via modulation of cytokines in the microenvironment, especially at the level of MIP-3α

Niobium effect on the Pzt ceramic properties

International Nuclear Information System (INIS)

Gimenes, R.; Zaguete, M.A.; Varela, J.A.; Cilense, M.; Paiva-Santos, C.O.; Las, W.C.

1996-01-01

Lead zirconate titanate, PZT, was prepared with Zr/Ti ratio of 50/50. The powder was prepared by the Pechini method with addition of 0,3; 0,5 e 0,7 mol % of Nb +5 ions. The precursors obtained were calcined at 700 deg C for 3 hs and milled with zirconia balls in a medium of isopropilic alcohol for 6 hs. The powders were characterized by XRD. For the sintering studies powders were isostatically pressed (230 MPa) into pellets and which were put inside a box furnace at 1200 deg C for 2 hs and 1100 deg C for 4 hs in a closed system containing 5 % of atmospheric powder (Pb Zr O 3 + 5% Pb O) relative to all pellets'mass. It was verified that the best densification rate was obtained for PZT with addition of 0,5 mol % of Nb +5 ions and sintered at 1100 deg C for 4 hs. Sintered samples at 1100 deg C for 4 hs were characterized as to dielectric (k, P r and E c ) and piezoelectric properties. (author)

Accurate double many-body expansion potential energy surface of HS2A2A′) by scaling the external correlation

International Nuclear Information System (INIS)

Zhang Lu-Lu; Song Yu-Zhi; Gao Shou-Bao; Zhang Yuan; Meng Qing-Tian

2016-01-01

A globally accurate single-sheeted double many-body expansion potential energy surface is reported for the first excited state of HS 2 by fitting the accurate ab initio energies, which are calculated at the multireference configuration interaction level with the aug-cc-pV Q Z basis set. By using the double many-body expansion-scaled external correlation method, such calculated ab initio energies are then slightly corrected by scaling their dynamical correlation. A grid of 2767 ab initio energies is used in the least-square fitting procedure with the total root-mean square deviation being 1.406 kcal·mol −1 . The topographical features of the HS 2 (A 2 A′) global potential energy surface are examined in detail. The attributes of the stationary points are presented and compared with the corresponding ab initio results as well as experimental and other theoretical data, showing good agreement. The resulting potential energy surface of HS 2 (A 2 A′) can be used as a building block for constructing the global potential energy surfaces of larger S/H molecular systems and recommended for dynamic studies on the title molecular system. (paper)

Headspace solid-phase microextraction (HS-SPME) and liquid-liquid extraction (LLE): comparison of the performance in classification of ecstasy tablets. Part 2.

Science.gov (United States)

Bonadio, Federica; Margot, Pierre; Delémont, Olivier; Esseiva, Pierre

2008-11-20

Headspace solid-phase microextraction (HS-SPME) is assessed as an alternative to liquid-liquid extraction (LLE) currently used for 3,4-methylenedioxymethampethamine (MDMA) profiling. Both methods were compared evaluating their performance in discriminating and classifying samples. For this purpose 62 different seizures were analysed using both extraction techniques followed by gas chromatography-mass spectroscopy (GC-MS). A previously validated method provided data for HS-SPME, whereas LLE data were collected applying a harmonized methodology developed and used in the European project CHAMP. After suitable pre-treatment, similarities between sample pairs were studied using the Pearson correlation. Both methods enable to distinguish between samples coming from the same pre-tabletting batches and samples coming from different pre-tabletting batches. This finding emphasizes the use of HS-SPME as an effective alternative to LLE, with additional advantages such as sample preparation and a solvent-free process.

FINITE ELEMENT ANALYSIS OF HASTELLOY C-22HS IN END MILLING

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K. Kadirgama

2011-12-01

Full Text Available This paper presents a finite element analysis of the stress distribution in the end milling operation of nickel-based superalloy HASTELLOY C-2000. Commercially available finite element software was used to develop the model and analyze the distribution of stress components in the machined surface of HASTELLOY C-22HS following end milling with coated carbide tools. The friction interaction along the tool-chip interface was modeled using the Coulomb friction law. It was found that the stress had lower values under the cut surface and that it increased gradually near the cutting edge.

Highly sensitive detection of 2,4,6-trichlorophenol based on HS-β-cyclodextrin/gold nanoparticles composites modified indium tin oxide electrode

International Nuclear Information System (INIS)

Zheng, Xiangli; Liu, Shan; Hua, Xiaoxia; Xia, Fangquan; Tian, Dong; Zhou, Changli

2015-01-01

Graphical abstract: Display Omitted -- Highlights: •A novel electrochemical sensing platform by self-assembling of HS-β-cyclodextrin/gold nanoparticles onto indium tin oxide electrode (HS-β-CD/AuNPs/SAM/ITO electrode) surface was constructed. •The proposed electrochemical sensor exhibited high sensitivity for the determination 2,4,6-trichlorophenol which electrochemical activity is very weak. •The newly developed method was successfully applied to quantitatively determine 2,4,6-trichlorophenol in tap water samples. -- ABSTRACT: A new electrochemical sensor for determination of 2,4,6-trichlorophenol (2,4,6-TCP) was fabricated. The characterization of the sensor was studied by scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry techniques. The electrochemical behavior of 2,4,6-TCP was investigated using cyclic voltammetry and differential pulse voltammetry at the HS-β-cyclodextrin (HS-β-CD)/gold nanoparticles (AuNPs) composite modified indium tin oxide (ITO) electrode. The results showed that the current responses of 2,4,6-TCP greatly enhanced due to the high catalytic activity and enrichment capability of composites. The peak current of 2,4,6-TCP increases linearly with the increase of the 2,4,6-TCP concentration from 3.0 × 10 −9 to 2.8 × 10 −8 M, with the limit of detection of 1.0 × 10 −9 . Further more, the modified electrode was successfully applied to detect the level of 2,4,6-TCP in tap water samples with excellent sensitivity

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China.

Science.gov (United States)

Liu, Junfeng; Wang, Deren; Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within 1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels (≥14 ng/L) and patients with normal hs-cTnT levels (mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies.

Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms.

Science.gov (United States)

Barazandeh Tehrani, Maliheh; Namadchian, Melika; Fadaye Vatan, Sedigheh; Souri, Effat

2013-04-10

A derivative spectrophotometric method was proposed for the simultaneous determination of clindamycin and tretinoin in pharmaceutical dosage forms. The measurement was achieved using the first and second derivative signals of clindamycin at (1D) 251 nm and (2D) 239 nm and tretinoin at (1D) 364 nm and (2D) 387 nm.The proposed method showed excellent linearity at both first and second derivative order in the range of 60-1200 and 1.25-25 μg/ml for clindamycin phosphate and tretinoin respectively. The within-day and between-day precision and accuracy was in acceptable range (CVpharmaceutical dosage form.

Identification of novel low-dose bisphenol a targets in human foreskin fibroblast cells derived from hypospadias patients.

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Xian-Yang Qin

Full Text Available The effect of low-dose bisphenol A (BPA exposure on human reproductive health is still controversial. To better understand the molecular basis of the effect of BPA on human reproductive health, a genome-wide screen was performed using human foreskin fibroblast cells (hFFCs derived from child hypospadias (HS patients to identify novel targets of low-dose BPA exposure.Gene expression profiles of hFFCs were measured after exposure to 10 nM BPA, 0.01 nM 17β-estradiol (E2 or 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD for 24 h. Differentially expressed genes were identified using an unpaired Student's t test with P value cut off at 0.05 and fold change of more than 1.2. These genes were selected for network generation and pathway analysis using Ingenuity Pathways Analysis, Pathway Express and KegArray. Seventy-one genes (42 downregulated and 29 upregulated were identified as significantly differentially expressed in response to BPA, among which 43 genes were found to be affected exclusively by BPA compared with E2 and TCDD. Of particular interest, real-time PCR analysis revealed that the expression of matrix metallopeptidase 11 (MMP11, a well-known effector of development and normal physiology, was found to be inhibited by BPA (0.47-fold and 0.37-fold at 10 nM and 100 nM, respectively. Furthermore, study of hFFCs derived from HS and cryptorchidism (CO patients (n = 23 and 11, respectively indicated that MMP11 expression was significantly lower in the HS group than in the CO group (0.25-fold, P = 0.0027.This present study suggests that an involvement of BPA in the etiology of HS might be associated with the downregulation of MMP11. Further study to elucidate the function of the novel target genes identified in this study during genital tubercle development might increase our knowledge of the effects of low-dose BPA exposure on human reproductive health.

The efficacy of radiofrequency ablation in the treatment of pediatric arrhythmia and its effects on serum IL-6 and hs-CRP.

Science.gov (United States)

Li, Chunli; Jia, Libo; Wang, Zhenzhou; Niu, Ling; An, Xinjiang

2017-10-01

The aim of this study was to investigate the efficacy of radiofrequency ablation in the treatment of pediatric arrhythmia and to assess the changes in serum interleukin-6 (IL-6) and hs-CRP levels after treatment. Hundred and six children with tachyarrhythmia who were admitted to Xuzhou Children's Hospital from November, 2014 to December, 2015 were recruited for study. The efficacies of radiofrequency in the treatment of different types of arrhythmia were analyzed. Successful ablation was found in 104 cases (98.11%) and recurrence was found in 7 cases (6.73%). Among 62 cases of atrioventricular reentrant tachycardia (AVRT), successful ablation was found in 60 cases (96.77%) and recurrence was found in 3 cases (4.84%). Among 33 cases of atrioventricular nodal reentrant tachycardia (AVNRT), successful ablation was found in 33 cases (100%) and recurrence was found in 2 cases (6.06%). Among 5 cases of ventricular tachycardia (VT), successful ablation was found in 5 cases (100%) and no recurrence was found. Among 4 cases of atrial tachycardia (AT), successful ablation was found in 4 cases (100%) and recurrence was found in 1 case (25%). Among 2 cases of atrial flutter (AFL), successful ablation was found in both (100%) and recurrence was found in 1 case (50%). After operation, the levels of IL-6 and hs-CRP were increased and were continually increased within 6 h after operation. The levels of IL-6 and hs-CRP at 24 h after operation were reduced but still higher than preoperative levels. The duration of radiofrequency and ablation energy were positively correlated with the levels of IL-6 and hs-CRP, while the number of discharges was not significantly correlated with either. In conclusion, radiofrequency ablation is a safe and effective treatment for pediatric arrhythmia. Postoperative monitoring of IL-6 and hs-CRP levels is conducive to understanding postoperative myocardial injury and inflammatory response.

Optimization of Biochemical Screening Methods for Volatile and Unstable Sesquiterpenoids Using HS-SPME-GC-MS

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Trine Bundgaard Andersen

2015-06-01

Full Text Available HS-SPME-GC-MS has been suggested as a fast and robust analytical platform for the product characterization of sesquiterpene synthases. The choice of fiber and injection temperature can have a significant effect on the observed product profile, due to the chemical rearrangements that can occur on the fiber material. Here we present a systematic study on the effects of fiber choice and injection port temperature on the observed sesquiterpenoid profile of four sesquiterpene synthases expressed in Nicotiana benthamiana. We found that the absorbent material PDMS was much less likely to support acid-induced rearrangement of sesquiterpenoids when compared to the adsorbent materials PDMS/DVB, PDMS/CAR, and PDMS/CAR/DVB. Furthermore, utilizing an injection port temperature at 160 °C almost eliminated the inherent thermal instability of germacrene sesquiterpenoids. Thus, for fast screening of sesquiterpene synthases, the results suggest that PDMS fibers and an injection temperature of 160 °C provide a fast and reproducible HS-SPME GC-MS method when using H2 as carrier gas.

Metabolism of isoeugenol via isoeugenol-diol by a newly isolated strain of Bacillus subtilis HS8.

Science.gov (United States)

Zhang, Yongmei; Xu, Ping; Han, Shuai; Yan, Haiqin; Ma, Cuiqing

2006-12-01

A bacterium designated as HS8 was newly isolated from soil based on its ability to degrade isoeugenol. The strain was identified as Bacillus subtilis according to its 16S rDNA sequence analysis and biochemical characteristics. The metabolic pathway for the degradation of isoeugenol was examined. Isoeugenol-diol, for the first time, was detected as an intermediate from isoeugenol to vanillin by a bacterial strain. Isoeugenol was converted to vanillin via isoeugenol-diol, and vanillin was then metabolized via vanillic acid to guaiacol by strain HS8. These metabolites, vanillin, vanillic acid, and guaiacol, are all valuable aromatic compounds in flavor production. At the same time, the bipolymerization of isoeugenol was observed, which produced dehydrodiisoeugenol and decreased the vanillin yield. High level of vanillic acid decarboxylase activity was detected in cell-free extract. These findings provided a detailed profile of isoeugenol metabolism by a B. subtilis strain for the first time, which would improve the production of valuable aromatic compounds by biotechnology.

Simultaneous determination of kolliphor HS15 and miglyol 812 in microemulsion formulation by ultra-high performance liquid chromatography coupled with nano quantity analyte detector

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Honggen Zhang

2016-02-01

Full Text Available A novel method for simultaneous determination of kolliphor HS15 and miglyol 812 in microemulsion formulation was developed using ultra-high performance liquid chromatography coupled with a nano quantitation analytical detector (UHPLC–NQAD. All components in kolliphor HS15 and miglyol 812 were well separated on an Acquity BEH C18 column. Mobile phase A was 0.1% trifluoroacetic acid (TFA in water and mobile phase B was acetonitrile. A gradient elution sequence was programed initially with 60% organic solvent, slowly increased to 100% within 8 min. The flow rate was 0.7 mL/min. Good linearity (r>0.95 was obtained in the range of 27.6–1381.1 μg/mL for polyoxyl 15 hydroxystearate in kolliphor HS15, 0.8–202.0 μg/mL for caprylic acid triglyceride and 2.7–221.9 μg/mL for capric acid triglyceride in miglyol 812. The relative standard deviations (RSD ranged from 0.6% to 1.7% for intra-day precision and from 0.4% to 2.7% for inter-day precision. The overall recoveries (accuracy were 99.7%–101.4% for polyoxyl 15 hydroxystearate in kolliphor HS15, 96.7%–99.6% for caprylic acid triglyceride, and 94.1%–103.3% for capric acid triglyceride in miglyol 812. Quantification limits (QL were determined as 27.6 μg/mL for polyoxyl 15 hydroxystearate in kolliphor HS15, 0.8 μg/mL for caprylic acid triglyceride, and 2.7 μg/mL for capric acid triglyceride in miglyol 812. No interferences were observed in the retention time ranges of kolliphor HS15 and miglyol 812. The method was validated in terms of specificity, linearity, precision, accuracy, QL, and robustness. The proposed method has been applied to microemulsion formulation analyses with good recoveries (82.2%–103.4%.

Simultaneous determination of kolliphor HS15 and miglyol 812 in microemulsion formulation by ultra-high performance liquid chromatography coupled with nano quantity analyte detector.

Science.gov (United States)

Zhang, Honggen; Wang, Zhenyu; Liu, Oscar

2016-02-01

A novel method for simultaneous determination of kolliphor HS15 and miglyol 812 in microemulsion formulation was developed using ultra-high performance liquid chromatography coupled with a nano quantitation analytical detector (UHPLC-NQAD). All components in kolliphor HS15 and miglyol 812 were well separated on an Acquity BEH C 18 column. Mobile phase A was 0.1% trifluoroacetic acid (TFA) in water and mobile phase B was acetonitrile. A gradient elution sequence was programed initially with 60% organic solvent, slowly increased to 100% within 8 min. The flow rate was 0.7 mL/min. Good linearity ( r >0.95) was obtained in the range of 27.6-1381.1 μg/mL for polyoxyl 15 hydroxystearate in kolliphor HS15, 0.8-202.0 μg/mL for caprylic acid triglyceride and 2.7-221.9 μg/mL for capric acid triglyceride in miglyol 812. The relative standard deviations (RSD) ranged from 0.6% to 1.7% for intra-day precision and from 0.4% to 2.7% for inter-day precision. The overall recoveries (accuracy) were 99.7%-101.4% for polyoxyl 15 hydroxystearate in kolliphor HS15, 96.7%-99.6% for caprylic acid triglyceride, and 94.1%-103.3% for capric acid triglyceride in miglyol 812. Quantification limits (QL) were determined as 27.6 μg/mL for polyoxyl 15 hydroxystearate in kolliphor HS15, 0.8 μg/mL for caprylic acid triglyceride, and 2.7 μg/mL for capric acid triglyceride in miglyol 812. No interferences were observed in the retention time ranges of kolliphor HS15 and miglyol 812. The method was validated in terms of specificity, linearity, precision, accuracy, QL, and robustness. The proposed method has been applied to microemulsion formulation analyses with good recoveries (82.2%-103.4%).

Clinical significance of determination of changes of serum IL-6, hs-CRP and saliva secretory IgA levels after treatment in patients with periodontitis

International Nuclear Information System (INIS)

Wang Tongwu

2009-01-01

Objective: To explore the clinical significance of changes of serum IL-6, hs-CRP and saliva secretory IgA levels after treatment in patients with periodontitis. Methods: Serum IL-6, saliva secretory IgA (with RIA) and serum hs-CRP (with immuno-tarbility method) levels were measured in 42 patients with periodontitis both before and after treatment as well as in 35 controls. Results: Before treatment serum IL-6, hs-CRP and saliva secretory IgA levels in the patients wree significantly higher than those in controls (P 0.05). However, the saliva secreatory IgA levels were still significantly higher than those in controls (P<0.05). Conclusion: There was disturbance of immunomodulation in patients with periodontitis as expressed by the changes of cytokines levels in the course of the diseases. (authors)

Scoring correction for MMPI-2 Hs scale with patients experiencing a traumatic brain injury: a test of measurement invariance.

Science.gov (United States)

Alkemade, Nathan; Bowden, Stephen C; Salzman, Louis

2015-02-01

It has been suggested that MMPI-2 scoring requires removal of some items when assessing patients after a traumatic brain injury (TBI). Gass (1991. MMPI-2 interpretation and closed head injury: A correction factor. Psychological assessment, 3, 27-31) proposed a correction procedure in line with the hypothesis that MMPI-2 endorsement may be affected by symptoms of TBI. This study assessed the validity of the Gass correction procedure. A sample of patients with a TBI (n = 242), and a random subset of the MMPI-2 normative sample (n = 1,786). The correction procedure implies a failure of measurement invariance across populations. This study examined measurement invariance of one of the MMPI-2 scales (Hs) that includes TBI correction items. A four-factor model of the MMPI-2 Hs items was defined. The factor model was found to meet the criteria for partial measurement invariance. Analysis of the change in sensitivity and specificity values implied by partial measurement invariance failed to indicate significant practical impact of partial invariance. Overall, the results support continued use of all Hs items to assess psychological well-being in patients with TBI. © The Author 2014. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Clinical significance of determination of the levels of serum IL-6, TNF-α and hs-CRP in patients with DM2 complicated with coronary heart disease (CHD)

International Nuclear Information System (INIS)

Li Juan; Zhao Qian; Zhang Dajun

2007-01-01

Objective: To investigate the significance of changes of serum IL-6, TNF-α, hs-CRP levels in development of coronary heart disease in patients with type 2 diabetes mellitus. Methods: Serum IL-6, TNF-α (with RIA). hs-CRP (with CLIA) and FPG, TG, cholesterol (with biochemistry) contents were measured in (1) DM2 patients complicated with CHD, n=40 and (2) DlVI2 patients without CHD, n=48. Results: The BMI and FPG, TG levels were significantly higher in patients complicated with heart disease than those in patients without CHD (P<0.05) with the exception of cholesterol. The levels of IL-6, TNF-α and hs-CRP were also significantly higher in patients with heart disease than those in patients without heart disease (P<0.05). Conclusion: The high levels of IL-6, TNF-α and hs-CRP played important role in the development of coronary heart disease. Monitoring those levels might be helpful in assessment of treatment efficacy and outcome prediction. (authors)

Reflection/suppression coatings for 900 - 1200 A radiation

Science.gov (United States)

Edelstein, Jerry

1989-01-01

The design and performance of multiple-layer, selective-reflection, selective-suppression coatings for the 900 - 1200 A band are described. These coatings are designed to optimize both high reflectivity at a desirable wavelength and low reflectivity at an undesirable wavelength. The minimum structure for a selective coating consists of a thin metal or metal oxide layer (50 - 150 A thickness) over an aluminum substrate protected with a semi-transparent dielectric (100 - 1000 A thickness). Predicted coating performance is strongly effected by varying the layer combination and thickness. A graphical method of optimizing the coating layer structure is developed. Aluminum, silicon, their oxides, and gold have been investigated as coating layer materials. A very simple coating with a 1026 to 1216 A reflectivity ratio greater than 100 was fabricated. Such reflection/suppression coatings may be of great utility to spaceborne EUV spectrographs.

HURRICANE AND SEVERE STORM SENTINEL (HS3) GLOBAL HAWK ADVANCED VERTICAL ATMOSPHERIC PROFILING SYSTEM (AVAPS) DROPSONDE SYSTEM V2

Data.gov (United States)

National Aeronautics and Space Administration — The Hurricane and Severe Storm Sentinel (HS3) Global Hawk Advanced Vertical Atmospheric Profiling System (AVAPS) Dropsonde System dataset was collected by the...

An efficient approach for electric load forecasting using distributed ART (adaptive resonance theory) and HS-ARTMAP (Hyper-spherical ARTMAP network) neural network

International Nuclear Information System (INIS)

Cai, Yuan; Wang, Jian-zhou; Tang, Yun; Yang, Yu-chen

2011-01-01

This paper presents a neural network based on adaptive resonance theory, named distributed ART (adaptive resonance theory) and HS-ARTMAP (Hyper-spherical ARTMAP network), applied to the electric load forecasting problem. The distributed ART combines the stable fast learning capabilities of winner-take-all ART systems with the noise tolerance and code compression capabilities of multi-layer perceptions. The HS-ARTMAP, a hybrid of an RBF (Radial Basis Function)-network-like module which uses hyper-sphere basis function substitute the Gaussian basis function and an ART-like module, performs incremental learning capabilities in function approximation problem. The HS-ARTMAP only receives the compressed distributed coding processed by distributed ART to deal with the proliferation problem which ARTMAP (adaptive resonance theory map) architecture often encounters and still performs well in electric load forecasting. To demonstrate the performance of the methodology, data from New South Wales and Victoria in Australia are illustrated. Results show that the developed method is much better than the traditional BP and single HS-ARTMAP neural network. -- Research highlights: → The processing of the presented network is based on compressed distributed data. It's an innovation among the adaptive resonance theory architecture. → The presented network decreases the proliferation the Fuzzy ARTMAP architectures usually encounter. → The network on-line forecasts electrical load accurately, stably. → Both one-period and multi-period load forecasting are executed using data of different cities.

The influence of statin treatment on the inflammatory biomarkers YKL-40 and HsCRP in patients with stable coronary artery disease

DEFF Research Database (Denmark)

Mygind, Naja Dam; Harutyunyan, Marina J; Mathiasen, Anders Bruun

2011-01-01

OBJECTIVE: The inflammatory biomarker YKL-40 is elevated and associated with mortality in patients with stable coronary artery disease (CAD). The aim was to investigate the influence of statin treatment and lipid status on serum YKL-40 and Hs-CRP in patients with stable CAD. DESIGN: Serum YKL-40......, HsCRP, total cholesterol, HDL-c, LDL-c and triglycerides levels were measured in 404 statin treated and in 404 matched non-statin treated patients with stable CAD. RESULTS: YKL-40 was significantly higher in non-statin treated 110 µg/l (median) compared with 65 µg/l in statin treated (p ...

Two-photon laser-induced fluorescence studies of HS radicals, DS radicals, and I atoms

Energy Technology Data Exchange (ETDEWEB)

Tiee, J J; Ferris, M J; Loge, G W; Wampler, F B

1983-04-15

A two-photon laser-induced excitation and fluorescence technique has been used to study the A /sup 2/..sigma../sup +/ - X/sup 2/PI transition of HS and DS radicals and various high-lying /sup 4/P/sup 0/, /sup 2/D/sup 0/, and /sup 4/D/sup 0/ states of the I atom. The two-photon excitation cross sections and detection sensitivity are discussed. 13 references, 5 figures.

Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes.

Science.gov (United States)

Ko, Hyun-Sun; Jin, Rong-De; Krishnan, Hari B; Lee, Sang-Bog; Kim, Kil-Yong

2009-12-01

Several rhizobacteria play a vital role in plant protection, plant growth promotion and the improvement of soil health. In this study, we have isolated a strain of Lysobacter antibioticus HS124 from rhizosphere and demonstrate its antifungal activity against various pathogens including Phytophthora capsici, a destructive pathogen of pepper plants. L. antibioticus HS124 produced lytic enzymes such as chitinase, beta-1,3-glucanase, lipase, protease, and an antibiotic compound. This antibiotic compound was purified by diaion HP-20, silica gel, sephadex LH-20 column chromatography and high performance liquid chromatography. The purified compound was identified as 4-hydroxyphenylacetic acid by gas chromatography-electron ionization (GC-EI) and gas chromatography-chemical ionization (GC-CI) mass spectrometry. This antibiotic exhibited destructive activity toward P. capsici hyphae. In vivo experiments utilizing green house grown pepper plants demonstrated the protective effect of L. antibioticus HS124 against P. capsici. The growth of pepper plants treated with L. antibioticus culture was enhanced, resulting in greater protection from fungal disease. Optimum growth and protection was found when cultures were grown in presence of Fe(III). Additionally, the activities of pathogenesis-related proteins such as chitinase and beta-1,3-glucanase decreased in roots, but increased in leaves with time after treatment compared to controls. Our results demonstrate L. antibioticus HS124 as a promising candidate for biocontrol of P. capsici in pepper plants.

Pilates versus resistance exercise on the serum levels of hs-CRP, in the abdominal circumference and body mass index (BMI in elderly individuals

Directory of Open Access Journals (Sweden)

Maria Adesilda Silva Pestana

2016-06-01

Full Text Available Recent studies have shown that the elderly exhibit a subclinical state of inflammation associated with increased adipose tissue and several comorbidities. To compare the effects of mat Pilates based exercises and resistance exercise on the serum levels of reactive C protein of high sensitivity (hs-CRP, in the abdominal circumference (AC and the body mass index (BMI in the elderly. It is a randomised clinical trial with a sample of 78 elderly individuals (median age 69 years. The active independent variable investigated was nature of treatment intervention (Pilates mat based exercises vs resistance exercise, and the dependent variables were hs-CRP level, AC and BMI. The statistical analysis used Wilcoxon signed rank and Mann-Whitney tests. The correlation between the continuous variables was assessed using Spearman’s coefficient of correlation. The data were analysed using SPSS software version 17.0, and probability values lower than 5% (p< 0.05 were considered statistically significant. Mat Pilates based exercises exhibited reductions in serum hs-CPR level (Wilcoxon signed rank test; z = -2.466, p = 0.01, BMI (Wilcoxon signed rank test; z = -3.295, p = 0.001 and AC (Wilcoxon signed rank test; z = -3.398, p = 0.01. Mat Pilates based exercises promoted a significant reduction of the serum hs-CRP levels and anthropometric measurements in elderly individuals.

Phase change of iron ore reduction process using EFB as reducing agent at 900-1200°C

Science.gov (United States)

Purwanto, H.; Salleh, H. M.; Rozhan, A. N.; Mohamad, A. S.; Zakiyuddin, A.

2018-04-01

Treatment of low grade iron ore involved reduction of oxygen in iron oxide by using reductant such as carbon monoxide or hydrogen gas. Presently, carboneous materials such as coke/coal are widely used as a source to provide reducing gas, but some problem arises from this material as the gas can harm the environments. Therefore, empty fruit bunch biomass from oil palm becomes an alternative to replace the usage of coke/coal as their major composition is carbon and hydrogen. The idea of replacing coke with biomass will reduce the amount of carbon dioxide release as biomass is a carbon neutral and renewable source, and at the same time abundance of waste from oil palm industries can be overcome. Therefore, the aim of this research is to upgrade the low grade iron with reducibility more than 50% being used in iron and steel making. In this research, low grade iron ore are mixed together with EFB then is making into composite pellet before being reduced at certain parameter chosen. The variables involved in this research is composition EFB (10%, 30% and 50%), temperature (1000°C, 1100°C and 1200°C) and reduction time is fixed with 30 minutes. From the experiment conducted, the highest reducibility achieved is 76.37% at temperature 1200°C. While XRD analysis shows the existence of metallic iron phase started to form at 1000°C with composition of 30% of EFB. Meanwhile, from magnetization test show that at 1200°C the highest magnetic susceptibility is achieved as the dominance phase at 1200°C is metallic phase. Therefore it is an interesting alternative to replace coke with biomass for reducing agent in upgrading low grade iron into workable ores.

Alfinated coating structure on HS6-5-2 (SW7M high speed steel

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T. Szymczak

2010-10-01

Full Text Available The paper presents the results of immersion alfinated coating structure in AlSi5 silumin on HS6-5-2 (SW7M high speed steel. Alfinating bath temperature was 750 ± 5 ° C, time of sample immersion was τ = 180s. Thickness of obtained coating under specified conditions was g = 150μm. Manufactured coating consists of three layers of different construction phase. The first layer from the substrate „g1`” constructed with a AlFe phase consist of alloy additives constituents of HS6-5-2 (SW7M steel: W, Mo, V, Cr and Si. On it crystallizes the second layer „g1``” of AlFeWMoCr intermetallic phases also containing Si and small amount of V. Last, the outer layer „g2” of the coating is composed with silumin including AlFeWMoCrVSi intermetallic phases. Within all layers of the coating occurs carbides. Penetration of carbides to individual coating layers is mainly due to steel surface partial melting and crystallizing layers „g1`” and „g1``” by alfinating liquid and shifting into her of carbides as well as partial carbides rejection by crystallization front of intermetallic phases occurs in coating.

Prevention of Lysosomal Storage Diseases and Derivation of Mutant Stem Cell Lines by Preimplantation Genetic Diagnosis

Science.gov (United States)

Altarescu, Gheona; Beeri, Rachel; Eiges, Rachel; Epsztejn-Litman, Silvina; Eldar-Geva, Talia; Elstein, Deborah; Zimran, Ari; Margalioth, Ehud J.; Levy-Lahad, Ephrat; Renbaum, Paul

2012-01-01

Preimplantation genetic diagnosis (PGD) allows birth of unaffected children for couples at risk for a genetic disorder. We present the strategy and outcome of PGD for four lysosomal storage disorders (LSD): Tay-Sachs disease (TSD), Gaucher disease (GD), Fabry disease (FD), and Hunter syndrome (HS), and subsequent development of stem cell lines. For each disease, we developed a family-specific fluorescent multiplex single-cell PCR protocol that included the familial mutation and informative markers surrounding the mutation. Embryo biopsy and PGD analysis were performed on either oocytes (polar bodies one and two) or on single blastomeres from a six-cell embryo. We treated twenty families carrying mutations in these lysosomal storage disorders, including 3 couples requiring simultaneous analysis for two disorders (TSD/GD, TSD/balanced Robertsonian translocation 45XYder(21;14), and HS/oculocutaneus albinism). These analyses led to an overall pregnancy rate/embryo transfer of 38% and the birth of 20 unaffected children from 17 families. We have found that PGD for lysosomal disorders is a safe and effective method to prevent birth of affected children. In addition, by using mutant embryos for the derivation of stem cell lines, we have successfully established GD and HS hESC lines for use as valuable models in LSD research. PMID:23320174

Niobium effect on the Pzt ceramic properties; Efeito do niobio sobre as propriedades da ceramica PZT

Energy Technology Data Exchange (ETDEWEB)

Gimenes, R.; Zaguete, M.A.; Varela, J.A.; Cilense, M.; Paiva-Santos, C.O.; Las, W.C. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica

1996-12-31

Lead zirconate titanate, PZT, was prepared with Zr/Ti ratio of 50/50. The powder was prepared by the Pechini method with addition of 0,3; 0,5 e 0,7 mol % of Nb{sup +5} ions. The precursors obtained were calcined at 700 deg C for 3 hs and milled with zirconia balls in a medium of isopropilic alcohol for 6 hs. The powders were characterized by XRD. For the sintering studies powders were isostatically pressed (230 MPa) into pellets and which were put inside a box furnace at 1200 deg C for 2 hs and 1100 deg C for 4 hs in a closed system containing 5 % of atmospheric powder (Pb Zr O{sub 3} + 5% Pb O) relative to all pellets`mass. It was verified that the best densification rate was obtained for PZT with addition of 0,5 mol % of Nb{sup +5} ions and sintered at 1100 deg C for 4 hs. Sintered samples at 1100 deg C for 4 hs were characterized as to dielectric (k, P{sub r} and E{sub c}) and piezoelectric properties. (author) 5 refs., 2 figs., 3 tabs.

Measurement of Activity Coefficients at Infinite Dilution for Alcohols in [BMIM][CH3SO4] using HS-SPME/GC-FID

Directory of Open Access Journals (Sweden)

A. M. Elias

Full Text Available ABSTRACT The activity coefficient at infinite dilution (&IN1 and distribution ratios at infinite dilution (&IN2 were determined for alkanols (methanol, ethanol, 1-propanol, 1-butanol, 2-butanol, and 2-methyl-2-propanol in the ionic liquid (IL 1-butyl-3-methylimidazolium methyl sulfate ([BMIM][CH3SO4] by HS-SPME (Headspace - Solid Phase Micro Extraction at four temperatures (298.15, 313.15, 333.15, and 353.15K using headspace - solid phase microextraction (SPME-HS. The results showed significant agreement with literature data. In addition, partial molar excess enthalpies at infinite dilution (&IN3, excess Gibbs energies (&IN4, and excess entropies (&IN5 were calculated from the (&IN6 values.

A Headspace Solid Phase Microextraction (HS-SPME method for the chromatographic determination of alkylpyrazines in cocoa samples

Directory of Open Access Journals (Sweden)

Pini Gláucia F.

2004-01-01

Full Text Available A Headspace Solid Phase Microextraction (HS-SPME procedure for isolation and determination of alkylpyrazines in cocoa liquor, using Gas Chromatography with Flame Ionization Detection (GC-FID for the separation and detection of the analytes, is presented here. The HS-SPME operational conditions were optimized using extractions of samples spiked with known amounts of alkylpyrazines typically found on cocoa products. The maximum extraction efficiency was obtained using SPME fibers coated with 65 µm Carbowax/divinylbenzene. Additionally, the best results were achieved with extraction temperature of 60 ºC, 15 min of sample/headspace equilibration time and 45 min extraction time. It was also observed that suspending the samples in saturated aqueous NaCl solution during extractions resulted in a significant increment on the peak areas. This procedure was found to be effective to determine the so-called pyrazinic ratios (quotient between peak areas of alkylpyrazines, which are useful as quality parameters for cocoa liquor.

Simultaneous determination of kolliphor HS15 and miglyol 812 in microemulsion formulation by ultra-high performance liquid chromatography coupled with nano quantity analyte detector

OpenAIRE

Honggen Zhang; Zhenyu Wang; Oscar Liu

2016-01-01

A novel method for simultaneous determination of kolliphor HS15 and miglyol 812 in microemulsion formulation was developed using ultra-high performance liquid chromatography coupled with a nano quantitation analytical detector (UHPLC–NQAD). All components in kolliphor HS15 and miglyol 812 were well separated on an Acquity BEH C18 column. Mobile phase A was 0.1% trifluoroacetic acid (TFA) in water and mobile phase B was acetonitrile. A gradient elution sequence was programed initially with 60%...

Quantification of Polyfunctional Thiols in Wine by HS-SPME-GC-MS Following Extractive Alkylation.

Science.gov (United States)

Musumeci, Lauren E; Ryona, Imelda; Pan, Bruce S; Loscos, Natalia; Feng, Hui; Cleary, Michael T; Sacks, Gavin L

2015-07-06

Analyses of key odorous polyfunctional volatile thiols in wines (3-mercaptohexanol (3-MH), 3-mercaptohexylacetate (3-MHA), and 4-mercapto-4-methyl-2-pentanone (4-MMP)) are challenging due to their high reactivity and ultra-trace concentrations, especially when using conventional gas-chromatography electron impact mass spectrometry (GC-EI-MS). We describe a method in which thiols are converted to pentafluorobenzyl (PFB) derivatives by extractive alkylation and the organic layer is evaporated prior to headspace solid phase microextraction (HS-SPME) and GC-EI-MS analysis. Optimal parameters were determined by response surface area modeling. The addition of NaCl solution to the dried SPME vials prior to extraction resulted in up to less than fivefold improvement in detection limits. Using 40 mL wine samples, limits of detection for 4-MMP, 3-MH, and 3-MHA were 0.9 ng/L, 1 ng/L, and 17 ng/L, respectively. Good recovery (90%-109%) and precision (5%-11% RSD) were achieved in wine matrices. The new method was used to survey polyfunctional thiol concentrations in 61 commercial California and New York State wines produced from V. vinifera (Riesling, Gewürztraminer, Cabernet Sauvignon, Sauvignon blanc and non-varietal rosé wines), V. labruscana (Niagara), and Vitis spp. (Cayuga White). Mean 4-MMP concentrations in New York Niagara (17 ng/L) were not significantly different from concentrations in Sauvignon blanc, but were significantly higher than 4-MMP in other varietal wines.

Quantification of Polyfunctional Thiols in Wine by HS-SPME-GC-MS Following Extractive Alkylation

Directory of Open Access Journals (Sweden)

Lauren E. Musumeci

2015-07-01

Full Text Available Analyses of key odorous polyfunctional volatile thiols in wines (3-mercaptohexanol (3-MH, 3-mercaptohexylacetate (3-MHA, and 4-mercapto-4-methyl-2-pentanone (4-MMP are challenging due to their high reactivity and ultra-trace concentrations, especially when using conventional gas-chromatography electron impact mass spectrometry (GC-EI-MS. We describe a method in which thiols are converted to pentafluorobenzyl (PFB derivatives by extractive alkylation and the organic layer is evaporated prior to headspace solid phase microextraction (HS-SPME and GC-EI-MS analysis. Optimal parameters were determined by response surface area modeling. The addition of NaCl solution to the dried SPME vials prior to extraction resulted in up to less than fivefold improvement in detection limits. Using 40 mL wine samples, limits of detection for 4-MMP, 3-MH, and 3-MHA were 0.9 ng/L, 1 ng/L, and 17 ng/L, respectively. Good recovery (90%–109% and precision (5%–11% RSD were achieved in wine matrices. The new method was used to survey polyfunctional thiol concentrations in 61 commercial California and New York State wines produced from V. vinifera (Riesling, Gewürztraminer, Cabernet Sauvignon, Sauvignon blanc and non-varietal rosé wines, V. labruscana (Niagara, and Vitis spp. (Cayuga White. Mean 4-MMP concentrations in New York Niagara (17 ng/L were not significantly different from concentrations in Sauvignon blanc, but were significantly higher than 4-MMP in other varietal wines.

Effects of Atorvastatin calcium combined with Aspirin on serum levels of Hcy, NSE, UA, hs-CRP and inflammatory factors of patients with cerebral infarction

Directory of Open Access Journals (Sweden)

Shu-Qin Zhang

2017-03-01

Full Text Available Objective: To study the effects of Atorvastatin calcium combined with Aspirin on serum levels of homocysteine (Hcy, neuron-specific enolase (NSE, uric acid (UA, high sensitity C-reactive protein (hs-CRP and inflammatory factors of patients with cerebral infarction. Methods: 100 cases of patients with cerebral infarction from March 2014 to May 2016 were treated in the Department of Neurology of our hospital and affiliated to Huazhong University of Science and Technology of traditional Chinese medicine and Western Medicine. The subjects were divided into the control group (n=50 and the treatment group (n=50 randomly. The control group was treated with Aspirin, the treatment group were treated with Atorvastatin calcium combined with Aspirin. The two groups were treated for 28 d. The serum levels of Hcy, NSE, UA, hs- CRP, interleukin-6 (IL-6, interleukin-8 (IL-8 and tumor necrosis factor-α (TNF-α of the two groups before and after treatment were compared. Results: There were no significantly differences of the serum levels of the Hcy, NSE, UA and hs-CRP of the two groups before treatment (P>0.05. After treatment, the serum levels of the Hcy, NSE, UA and hs-CRP of the two groups were significantly lower than before treatment, and that of the treatment group were significantly lower than the control group (P0.05. After treatment, the serum levels of the IL-6, IL-8 and TNF-α of the two groups were significantly lower than before treatment, and that of the treatment group were significantly lower than the control group (P<0.05. Conclusions: Atorvastatin calcium combined with Aspirin can significantly reduce the serum levels of Hcy, NSE, UA, hs-CRP, IL-6, IL-8 and TNF-α of the patients with cerebral infarction.

Evaluation of cell count and classification capabilities in body fluids using a fully automated Sysmex XN equipped with high-sensitive Analysis (hsA) mode and DI-60 hematology analyzer system.

Science.gov (United States)

Takemura, Hiroyuki; Ai, Tomohiko; Kimura, Konobu; Nagasaka, Kaori; Takahashi, Toshihiro; Tsuchiya, Koji; Yang, Haeun; Konishi, Aya; Uchihashi, Kinya; Horii, Takashi; Tabe, Yoko; Ohsaka, Akimichi

2018-01-01

The XN series automated hematology analyzer has been equipped with a body fluid (BF) mode to count and differentiate leukocytes in BF samples including cerebrospinal fluid (CSF). However, its diagnostic accuracy is not reliable for CSF samples with low cell concentration at the border between normal and pathologic level. To overcome this limitation, a new flow cytometry-based technology, termed "high sensitive analysis (hsA) mode," has been developed. In addition, the XN series analyzer has been equipped with the automated digital cell imaging analyzer DI-60 to classify cell morphology including normal leukocytes differential and abnormal malignant cells detection. Using various BF samples, we evaluated the performance of the XN-hsA mode and DI-60 compared to manual microscopic examination. The reproducibility of the XN-hsA mode showed good results in samples with low cell densities (coefficient of variation; % CV: 7.8% for 6 cells/μL). The linearity of the XN-hsA mode was established up to 938 cells/μL. The cell number obtained using the XN-hsA mode correlated highly with the corresponding microscopic examination. Good correlation was also observed between the DI-60 analyses and manual microscopic classification for all leukocyte types, except monocytes. In conclusion, the combined use of cell counting with the XN-hsA mode and automated morphological analyses using the DI-60 mode is potentially useful for the automated analysis of BF cells.

Evaluating watershed protection programs in New York City's Cannonsville Reservoir source watershed using SWAT-HS

Science.gov (United States)

Hoang, L.; Mukundan, R.; Moore, K. E.; Owens, E. M.; Steenhuis, T. S.

2017-12-01

New York City (NYC)'s reservoirs supply over one billion gallons of drinking water each day to over nine million consumers in NYC and upstate communities. The City has invested more than $1.5 billion in watershed protection programs to maintain a waiver from filtration for the Catskill and Delaware Systems. In the last 25 years, the NYC Department of Environmental Protection (NYCDEP) has implemented programs in cooperation with upstate communities that include nutrient management, crop rotations, improvement of barnyards and manure storage, implementing tertiary treatment for Phosphorus (P) in wastewater treatment plants, and replacing failed septic systems in an effort to reduce P loads to water supply reservoirs. There have been several modeling studies evaluating the effect of agricultural Best Management Practices (BMPs) on P control in the Cannonsville watershed in the Delaware System. Although these studies showed that BMPs would reduce dissolved P losses, they were limited to farm-scale or watershed-scale estimates of reduction factors without consideration of the dynamic nature of overland flow and P losses from variable source areas. Recently, we developed the process-based SWAT-Hillslope (SWAT-HS) model, a modified version of the Soil and Water Assessment Tool (SWAT) that can realistically predict variable source runoff processes. The objective of this study is to use the SWAT-HS model to evaluate watershed protection programs addressing both point and non-point sources of P. SWAT-HS predicts streamflow very well for the Cannonsville watershed with a daily Nash Sutcliffe Efficiency (NSE) of 0.85 at the watershed outlet and NSE values ranging from 0.56 - 0.82 at five other locations within the watershed. Based on good hydrological prediction, we applied the model to predict P loads using detailed P inputs that change over time due to the implementation of watershed protection programs. Results from P model predictions provide improved projections of P

Evaluation of Hs-CRP levels and interleukin 18 (-137G/C promoter polymorphism in risk prediction of coronary artery disease in first degree relatives.

Directory of Open Access Journals (Sweden)

Rajesh Kumar G

Full Text Available Coronary Artery Disease (CAD is clearly a multifactorial disease that develops from childhood and ultimately leads to death. Several reports revealed having a First Degree Relatives (FDRS with premature CAD is a significant autonomous risk factor for CAD development. C - reactive protein (CRP is a member of the pentraxin family and is the most widely studied proinflammatory biomarker. IL-18 is a pleiotrophic and proinflammatory cytokine which is produced mainly by macrophages and plays an important role in the inflammatory cascade.Hs-CRP levels were estimated by ELISA and Genotyping of IL-18 gene variant located on promoter -137 (G/C by Allele specific PCR in blood samples of 300 CAD patients and 300 controls and 100 FDRS. Promoter Binding sites and Protein interacting partners were identified by Alibaba 2.1 and Genemania online tools respectively. Hs-CRP levels were significantly high in CAD patients followed by FDRS when compared to controls. In IL-18 -137 (G/C polymorphism homozygous GG is significantly associated with occurrence of CAD and Hs-CRP levels were significantly higher in GG genotype subjects when compared to GC and CC. IL-18 was found to be interacting with 100 protein interactants.Our results indicate that Hs-CRP levels and IL-18-137(G/C polymorphism may help to identify risk of future events of CAD in asymptomatic healthy FDRS.

Evaluation of Hs-CRP levels and interleukin 18 (-137G/C) promoter polymorphism in risk prediction of coronary artery disease in first degree relatives.

Science.gov (United States)

G, Rajesh Kumar; K, Mrudula Spurthi; G, Kishore Kumar; Kurapati, Mohanalatha; M, Saraswati; T, Mohini Aiyengar; P, Chiranjeevi; G, Srilatha Reddy; S, Nivas; P, Kaushik; K, Sanjib Sahu; H, Surekha Rani

2015-01-01

Coronary Artery Disease (CAD) is clearly a multifactorial disease that develops from childhood and ultimately leads to death. Several reports revealed having a First Degree Relatives (FDRS) with premature CAD is a significant autonomous risk factor for CAD development. C - reactive protein (CRP) is a member of the pentraxin family and is the most widely studied proinflammatory biomarker. IL-18 is a pleiotrophic and proinflammatory cytokine which is produced mainly by macrophages and plays an important role in the inflammatory cascade. Hs-CRP levels were estimated by ELISA and Genotyping of IL-18 gene variant located on promoter -137 (G/C) by Allele specific PCR in blood samples of 300 CAD patients and 300 controls and 100 FDRS. Promoter Binding sites and Protein interacting partners were identified by Alibaba 2.1 and Genemania online tools respectively. Hs-CRP levels were significantly high in CAD patients followed by FDRS when compared to controls. In IL-18 -137 (G/C) polymorphism homozygous GG is significantly associated with occurrence of CAD and Hs-CRP levels were significantly higher in GG genotype subjects when compared to GC and CC. IL-18 was found to be interacting with 100 protein interactants. Our results indicate that Hs-CRP levels and IL-18-137(G/C) polymorphism may help to identify risk of future events of CAD in asymptomatic healthy FDRS.

Prognostic impact of hs-CRP and IL-6 in patients with persistent atrial fibrillation treated with electrical cardioversion

DEFF Research Database (Denmark)

Henningsen, Kristoffer Mads Aaris; Therkelsen, Susette Krohn; Bruunsgaard, Helle

2009-01-01

OBJECTIVE: The aim of this study was to assess the role of inflammatory processes in the development of atrial fibrillation (AF) and the prognostic impact of inflammatory markers in predicting long-term risk of AF recurrence after electrical cardioversion (CV). METHODS: High-sensitivity C......-reactive protein (hs-CRP) and interleukin-6 (IL-6) were measured in 56 patients with persistent AF (lasting mean 128 days (range 14-960), mean age 65 years (34-84)), 19 healthy volunteers and 19 patients with permanent AF. Patients with persistent AF underwent CV. Blood samples were taken prior to CV and after 1......, 30 and 180 days. RESULTS: The immediate success rate of CV was 88%, while the total recurrence rate after 180 days was 68%. Patients with permanent AF had significantly higher levels of hs-CRP and IL-6 than patients with persistent AF (p = 0.0011, p

The new isotope 270110 and its decay products 266Hs and 262Sg

International Nuclear Information System (INIS)

Hofmann, S.; Hessberger, F.P.; Ackermann, D.

2000-11-01

The even-even nucleus 270 110 was synthesized using the reaction 64 Ni + 207 Pb. A total of eight α-decay chains was measured during an irradiation time of seven days. Decay data were obtained for the ground-state and a high spin K isomer. The new nuclei 266 Hs and 262 Sg were identified as daughter products after α decay. Spontaneous fission of 262 Sg terminates the decay chain. The measured data are in agreement with calculations using the macroscopic-microscopic model and with self-consistent HFB calculations with Skyrme-Sly4 interaction. (orig.)

[History of induced abortion in Denmark from 1200 to 1979].

Science.gov (United States)

Manniche, E

1982-10-01

History of induced abortion in Denmark from 1200 to 1979 is reviewed. The 1st Danish law of 1200 did not touch upon the question of induced abortion. From the beginning of the 13th century to Religious Reformation in 1536, Roman Catholic law influenced every aspect of Danish life including induced abortion. In 1683 in King Christian V's constitution called Dansk Lov induced abortion was discussed. Immoral women who aborted fetuses or killed newborn babies were decapitated. In Copenhagen in the years 1624-1632 and 1638-1663 17 women were executed because of induced abortion or murder of newborn babies. Although Dansk Lov was effective till 1866, Danish kings came to treat female criminals less severely since about 1780-1800. For example, between 1855 and 1866 42 women convicted of murder of newborn babies or abortion were given pardon (12 years of imprisonment instead of life sentence). In 1866, abortion and murder of babies were treated separately in the Danish criminal law. Induced abortion meant up to 8 years of imprisonment and labor. In 1930 life sentence was abolished; induced abortion called for only up to 2 years of imprisonment, while those who assisted for money were punished more severely (up to 8 years in prison). In 1937 the Danes legalized induced abortion for medical, ethical, (e.g. rape case) and eugenic reasons. By 1973 legalized abortion was available, free of charge, to every Danish female resident within 12 weeks of pregnancy. In 1980 abortion rate was about 41% of total births. It is estimated 2/3 of Danish women experience abortion. Lastly, illegitimate births and miscarriages are on the rise due to changes in women's social status and role.

Hybrid clone cells derived from human breast epithelial cells and human breast cancer cells exhibit properties of cancer stem/initiating cells.

Science.gov (United States)

Gauck, Daria; Keil, Silvia; Niggemann, Bernd; Zänker, Kurt S; Dittmar, Thomas

2017-08-02

The biological phenomenon of cell fusion has been associated with cancer progression since it was determined that normal cell × tumor cell fusion-derived hybrid cells could exhibit novel properties, such as enhanced metastatogenic capacity or increased drug resistance, and even as a mechanism that could give rise to cancer stem/initiating cells (CS/ICs). CS/ICs have been proposed as cancer cells that exhibit stem cell properties, including the ability to (re)initiate tumor growth. Five M13HS hybrid clone cells, which originated from spontaneous cell fusion events between M13SV1-EGFP-Neo human breast epithelial cells and HS578T-Hyg human breast cancer cells, and their parental cells were analyzed for expression of stemness and EMT-related marker proteins by Western blot analysis and confocal laser scanning microscopy. The frequency of ALDH1-positive cells was determined by flow cytometry using AldeRed fluorescent dye. Concurrently, the cells' colony forming capabilities as well as the cells' abilities to form mammospheres were investigated. The migratory activity of the cells was analyzed using a 3D collagen matrix migration assay. M13HS hybrid clone cells co-expressed SOX9, SLUG, CK8 and CK14, which were differently expressed in parental cells. A variation in the ALDH1-positive putative stem cell population was observed among the five hybrids ranging from 1.44% (M13HS-7) to 13.68% (M13HS-2). In comparison to the parental cells, all five hybrid clone cells possessed increased but also unique colony formation and mammosphere formation capabilities. M13HS-4 hybrid clone cells exhibited the highest colony formation capacity and second highest mammosphere formation capacity of all hybrids, whereby the mean diameter of the mammospheres was comparable to the parental cells. In contrast, the largest mammospheres originated from the M13HS-2 hybrid clone cells, whereas these cells' mammosphere formation capacity was comparable to the parental breast cancer cells. All M13HS

A large multi-centre European study validates high-sensitivity C-reactive protein (hsCRP) as a clinical biomarker for the diagnosis of diabetes subtypes

DEFF Research Database (Denmark)

Thanabalasingham, G.; Shah, N.; Vaxillaire, M.

2011-01-01

CRP) levels are lower in UK patients with hepatocyte nuclear factor 1 alpha (HNF1A)-MODY than in other diabetes subtypes. In this large multi-centre study we aimed to assess the clinical validity of hsCRP as a diagnostic biomarker, examine the genotype-phenotype relationship and compare different hsCRP assays....... High-sensitivity CRP levels were analysed in individuals with HNF1A-MODY (n = 457), glucokinase (GCK)-MODY (n = 404), hepatocyte nuclear factor 4 alpha (HNF4A)-MODY (n = 54) and type 2 diabetes (n = 582) from seven European centres. Three common assays for hsCRP analysis were evaluated. We excluded 121......) a parts per thousand yenaEuro parts per thousand 0.91, p a parts per thousand currency signaEuro parts per thousand 1 x 10(-5)). Across the seven centres, the C-statistic for distinguishing HNF1A-MODY from young adult-onset type 2 diabetes ranged from 0.79 to 0.97, indicating high discriminative accuracy...

Cytokines, brain-derived neurotrophic factor and C-reactive protein in bipolar I disorder

DEFF Research Database (Denmark)

Jacoby, Anne Sophie; Munkholm, Klaus; Vinberg, Maj

2016-01-01

BACKGROUND: Peripheral blood brain-derived neurotrophic factor (BDNF) and inflammatory markers may reflect key pathophysiological mechanisms in bipolar disorder in relation to disease activity and neuroprogression. AIMS: To investigate whether neutrophins and inflammatory marker vary with mood...... overall compared with healthy control subjects. However, in adjusted models, no statistically significant differences were found in any measure between patients and control individuals. Levels of hsCRP in depressive states were decreased with 40% (95% CI: 5-62%, p=0.029) compared with euthymia and with 48...

Plasticity and fracture modeling of three-layer steel composite Tribond® 1200 for crash simulation

NARCIS (Netherlands)

Eller, Tom; Ramaker, Kenny; Greve, Lars; Andres, M.T.; Hazrati Marangalou, Javad; van den Boogaard, Antonius H.

2017-01-01

A constitutive model is presented for the three-layer steel composite Tribond® 1200. Tribond® is a hot forming steel which consists of three layers: a high strength steel core between two outer layers of ductile low strength steel. The model is designed to provide an accurate prediction of the

Reproducibility of disc and macula optical coherence tomography using the Canon OCT-HS100 as compared with the Zeiss Cirrus HD-OCT.

Science.gov (United States)

Brautaset, Rune; Birkeldh, Ulrika; Rosén, Rebecka; Ramsay, Marika Wahlberg; Nilsson, Maria

2014-01-01

In a clinical setting, the usefulness of optical coherence tomography (OCT) is strongly dependent on reproducibility of the measurement. The aim of the present study was to evaluate macula and optic disc measurement reproducibility with the new spectral-domain OCT (SD-OCT) from Canon (Canon OCT-HS100) and to compare reproducibility and obtained measurements with the Zeiss Cirrus HD-OCT. Macula and optic disc parameters from the right eyes of 31 subjects were obtained twice with both instruments. Interoperator reproducibility was evaluated by use of the coefficient of repeatability (CR), and the obtained measurements were compared between the instruments. No difference in interoperator reproducibility could be found when comparing the 2 instruments and reproducibility ranged from 3.94% to 12.77% for optic disc parameters and from 1.19% to 3.54% for macula parameters. The lowest reproducibility was found for cup volume and vertical cup/disc ratio with both instruments. For all macula and retinal nerve fiber layer (RNFL) thickness measurements, there was a statistical difference when comparing the 2 instruments, except for RFNL measurements of the superior quadrant, with the Canon OCT-HS100 always evaluating the thickness to be thicker; however, the 2 instruments correlated well. The Canon OCT-HS100 is a reproducible instrument that matches the Zeiss Cirrus HD-OCT well. It remains to be evaluated how sensitive the Canon OCT-HS100 is to detect small subtle changes in optic disc parameters and macular nerve fiber layer thickness. Furthermore, due to the differences in thickness estimation, it is important to emphasize that SD-OCTs are not interchangeable.

FINAL REPORT SUMMARY OF DM 1200 OPERATION AT VSL VSL-06R6710-2 REV 0 9/7/06

Energy Technology Data Exchange (ETDEWEB)

KRUGER AA; MATLACK KS; DIENER G; BARDAKCI T; PEGG IL

2011-12-29

The principal objective of this report was to summarize the testing experience on the DuraMelter 1200 (DMI200), which is the High Level Waste (HLW) Pilot Melter located at the Vitreous State Laboratory (VSL). Further objectives were to provide descriptions of the history of all modifications and maintenance, methods of operation, problems and unit failures, and melter emissions and performance while processing a variety of simulated HL W and low activity waste (LAW) feeds for the Hanford Waste Treatment and Immobilization Plant (WTP) and employing a variety of operating methods. All of these objectives were met. The River Protection Project - Hanford Waste Treatment and Immobilization Plant (RPP-WTP) Project has undertaken a 'tiered' approach to vitrification development testing involving computer-based glass formulation, glass property-composition models, crucible melts, and continuous melter tests of increasing, more realistic scales. Melter systems ranging from 0.02 to 1.2 m{sup 2} installed at the Vitreous State Laboratory (VSL) have been used for this purpose, which, in combination with the 3.3 m{sup 2} low activity waste (LAW) Pilot Melter at Duratek, Inc., span more than two orders of magnitude in melt surface area. In this way, less-costly small-scale tests can be used to define the most appropriate tests to be conducted at the larger scales in order to extract maximum benefit from the large-scale tests. For high level waste (HLW) vitrification development, a key component in this approach is the one-third scale DuraMelter 1200 (DM 1200), which is the HLW Pilot Melter that has been installed at VSL with an integrated prototypical off-gas treatment system. That system replaced the DM1000 system that was used for HLW throughput testing during Part B1. Both melters have similar melt surface areas (1.2 m{sup 2}) but the DM1200 is prototypical of the present RPP-WTP HLW melter design whereas the DM1000 was not. In particular, the DM1200 provides for

Final Report Summary Of DM 1200 Operation At VSL VSL-06R6710-2, Rev. 0, 9/7/06

International Nuclear Information System (INIS)

Kruger, A.A.; Matlack, K.S.; Diener, G.; Bardakci, T.; Pegg, I.L.

2011-01-01

The principal objective of this report was to summarize the testing experience on the DuraMelter 1200 (DMI200), which is the High Level Waste (HLW) Pilot Melter located at the Vitreous State Laboratory (VSL). Further objectives were to provide descriptions of the history of all modifications and maintenance, methods of operation, problems and unit failures, and melter emissions and performance while processing a variety of simulated HL W and low activity waste (LAW) feeds for the Hanford Waste Treatment and Immobilization Plant (WTP) and employing a variety of operating methods. All of these objectives were met. The River Protection Project - Hanford Waste Treatment and Immobilization Plant (RPP-WTP) Project has undertaken a 'tiered' approach to vitrification development testing involving computer-based glass formulation, glass property-composition models, crucible melts, and continuous melter tests of increasing, more realistic scales. Melter systems ranging from 0.02 to 1.2 m 2 installed at the Vitreous State Laboratory (VSL) have been used for this purpose, which, in combination with the 3.3 m 2 low activity waste (LAW) Pilot Melter at Duratek, Inc., span more than two orders of magnitude in melt surface area. In this way, less-costly small-scale tests can be used to define the most appropriate tests to be conducted at the larger scales in order to extract maximum benefit from the large-scale tests. For high level waste (HLW) vitrification development, a key component in this approach is the one-third scale DuraMelter 1200 (DM 1200), which is the HLW Pilot Melter that has been installed at VSL with an integrated prototypical off-gas treatment system. That system replaced the DM1000 system that was used for HLW throughput testing during Part B1. Both melters have similar melt surface areas (1.2 m 2 ) but the DM1200 is prototypical of the present RPP-WTP HLW melter design whereas the DM1000 was not. In particular, the DM1200 provides for testing on a vitrification

HS5 of the human β-globin Locus Control Region: a developmental stage-specific border in erythroid cells.

NARCIS (Netherlands)

A. Wai (Albert); N. Gillemans (Nynke); S. Raguz-Bolognesi (Selina); S. Pruzina (Sara); G. Zafarana (Gaetano); D.N. Meijer (Dies); F.G. Grosveld (Frank); J.N.J. Philipsen (Sjaak)

2003-01-01

textabstractElements with insulator/border activity have been characterized most extensively in Drosophila melanogaster. In vertebrates, the first example of such an element was provided by a hypersensitive site of the chicken beta-globin locus, cHS4. It has been proposed that the homologous site in

25 CFR 1200.3 - What is the Department's policy on tribal management of trust funds?

Science.gov (United States)

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false What is the Department's policy on tribal management of..., DEPARTMENT OF THE INTERIOR AMERICAN INDIAN TRUST FUND MANAGEMENT REFORM ACT General Provisions § 1200.3 What is the Department's policy on tribal management of trust funds? (a) We will give tribes as much...

Factors influencing the antifolate activity of synthetic tea-derived catechins.

Science.gov (United States)

Sáez-Ayala, Magalí; Fernández-Pérez, María Piedad; Chazarra, Soledad; Mchedlishvili, Nani; Tárraga-Tomás, Alberto; Rodríguez-López, José Neptuno

2013-07-16

Novel tea catechin derivatives have been synthesized, and a structure-activity study, related to the capacity of these and other polyphenols to bind dihydrofolate reductase (DHFR), has been performed. The data showed an effective binding between all molecules and the free enzyme, and the dissociation constants of the synthetic compounds and of the natural analogues were on the same order. Polyphenols with a catechin configuration were better DHFR inhibitors than those with an epicatechin configuration. Antiproliferative activity was also studied in cultured tumour cells, and the data showed that the activity of the novel derivatives was higher in catechin isomers. Derivatives with a hydroxyl group para on the ester-bonded gallate moiety presented a high in vitro binding to DHFR, but exhibited transport problems in cell culture due to ionization at physiologic pHs. The impact of the binding of catechins to serum albumin on their biological activity was also evaluated. The information provided in this study could be important for the design of novel medicinal active compounds derived from tea catechins. The data suggest that changes in their structure to avoid serum albumin interactions and to facilitate plasmatic membrane transport are essential for the intracellular functions of catechins.

HS-SPME-GC-MS ANALYSIS OF VOLATILE AND SEMI-VOLATILE COMPOUNDS FROM DRIED LEAVES OF Mikania glomerata Sprengel

Directory of Open Access Journals (Sweden)

Esmeraldo A. Cappelaro

2015-03-01

Full Text Available This paper reports on the identification of volatile and semi-volatile compounds and a comparison of the chromatographic profiles obtained by Headspace Solid-Phase Microextraction/Gas Chromatography with Mass Spectrometry detection (HS-SPME-GC-MS of dried leaves of Mikania glomerata Sprengel (Asteraceae, also known as 'guaco.' Three different types of commercial SPME fibers were tested: polydimethylsiloxane (PDMS, polydimethylsiloxane/divinylbenzene (PDMS/DVB and polyacrylate (PA. Fifty-nine compounds were fully identified by HS-SPME-HRGC-MS, including coumarin, a marker for the quality control of guaco-based phytomedicines; most of the other identified compounds were mono- and sesquiterpenes. PA fibers performed better in the analysis of coumarin, while PDMS-DVB proved to be the best choice for a general and non-selective analysis of volatile and semi-volatile guaco-based compounds. The SPME method is faster and requires a smaller sample than conventional hydrodistillation of essential oils, providing a general overview of the volatile and semi-volatile compounds of M. glomerata.

Effects of microbial DNA on human DNA profiles generated using the PowerPlex® 16 HS system.

Science.gov (United States)

Dembinski, Gina M; Picard, Christine J

2017-11-01

Most crime scenes are not sterile and therefore may be contaminated with environmental DNA, especially if a decomposing body is found. Collecting biological evidence from this individual will yield DNA samples mixed with microbial DNA. This also becomes important if postmortem swabs are collected from sexually assaulted victims. Although genotyping kits undergo validation tests, including bacterial screens, they do not account for the diverse microbial load during decomposition. We investigated the effect of spiking human DNA samples with known concentrations of DNA from 17 microbe species associated with decomposition on DNA profiles produced using the Promega PowerPlex ® HS system. Two species, Bacillus subtilis and Mycobacterium smegmatis, produced an extraneous allele at the TPOX locus. When repeated with the PowerPlex ® Fusion kit, the extra allele no longer amplified with these two species. This experiment demonstrates that caution should be exhibited if microbial load is high and the PowerPlex ® 16HS system is used. Copyright © 2017 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

The role of the oximes HI-6 and HS-6 inside human acetylcholinesterase inhibited with nerve agents: a computational study.

Science.gov (United States)

Cuya, Teobaldo; Gonçalves, Arlan da Silva; da Silva, Jorge Alberto Valle; Ramalho, Teodorico C; Kuca, Kamil; C C França, Tanos

2017-10-27

The oximes 4-carbamoyl-1-[({2-[(E)-(hydroxyimino) methyl] pyridinium-1-yl} methoxy) methyl] pyridinium (known as HI-6) and 3-carbamoyl-1-[({2-[(E)-(hydroxyimino) methyl] pyridinium-1-yl} methoxy) methyl] pyridinium (known as HS-6) are isomers differing from each other only by the position of the carbamoyl group on the pyridine ring. However, this slight difference was verified to be responsible for big differences in the percentual of reactivation of acetylcholinesterase (AChE) inhibited by the nerve agents tabun, sarin, cyclosarin, and VX. In order to try to find out the reason for this, a computational study involving molecular docking, molecular dynamics, and binding energies calculations, was performed on the binding modes of HI-6 and HS-6 on human AChE (HssAChE) inhibited by those nerve agents.

RoHS regulated substances in mixed plastics from waste electrical and electronic equipment.

Science.gov (United States)

Wäger, Patrick A; Schluep, Mathias; Müller, Esther; Gloor, Rolf

2012-01-17

The disposal and recovery of plastics from waste electrical and electronic equipment (WEEE) are of considerable importance, both from an environmental and an economic perspective. This paper presents the results of a study investigating current concentrations of hazardous substances in mixed plastics from WEEE and their implications for an environmentally sound recovery. The study included 53 sampling campaigns for mixed plastics from WEEE. The samples were analyzed with regard to heavy metals (cadmium, chromium, mercury, and lead) and flame retardants (PentaBDE, OctaBDE, DecaBDE, DecaBB) regulated in the RoHS Directive. Besides these substances, other brominated flame retardants known to occur in electronics (HBCD, TBBPA) as well as the total bromine and phosphorus contents were considered. Results show that no mixed plastics fraction from WEEE is completely free from substances regulated in the RoHS Directive. The lowest number and average concentrations were found in flat screen monitors. The highest concentrations were found in mixed plastics from CRT monitors and TVs. Mixed plastics fractions with high average concentrations of heavy metals originate from the treatment of small household appliances (cadmium), ICT equipment (lead), and consumer equipment (lead). Mixed plastics fractions with high average concentrations of brominated flame retardants mainly originate from the treatment of small household appliances for high temperature applications (DecaBDE), CRT monitors (OctaBDE and DecaBDE) and consumer equipment (DecaBDE), in particular CRT TVs (DecaBDE). To avoid a dissipation of hazardous substances into plastics and the environment, it is recommended that mixed plastics from WEEE are subject to a strict quality management.

Collisions with 1200 proton bunches in each beam recorded by the CMS detector April 2018

CERN Multimedia

Mc Cauley, Thomas

2018-01-01

These images depict collisions recorded by the CMS detector on 28 April 2018 from 1200 proton bunches in each circulating beam. The yellow lines represents reconstructed particle trajectories in the tracker. The green and blue rectangles represent energy deposits in the electromagnetic and hadronic calorimeters, respectively. The long red lines represent reconstructed muon trajectories.

Assessment of elder mistreatment in two American Indian samples: psychometric characteristics of the HS-EAST and the Native Elder Life-Financial Exploitation and -Neglect measures.

Science.gov (United States)

Jervis, Lori L; Fickenscher, Alexandra; Beals, Janette

2014-04-01

Although elder mistreatment among ethnic minorities is increasingly gaining attention, our empirical knowledge of this phenomenon among American Indians remains quite limited, especially with respect to measurement. The Shielding American Indian Elders (SAIE) Project used a collaborative approach to explore culturally informed measurement of elder mistreatment in two American Indian elder samples (a Northern Plains reservation and a South Central metropolitan area). The project sought to investigate the performance characteristics of the commonly used Hwalek-Sengstock Elder Abuse Screening Test (HS-EAST), as well as to examine the psychometric properties of a new measure developed to capture culturally salient aspects of mistreatment in American Indian contexts--the Native Elder Life Scale (NELS). Using methods and samples comparable to those in the literature, the HS-EAST performed adequately in these Native samples. The NELS also shows promise for use with this population and assesses different aspects of elder mistreatment than does the HS-EAST.

Significant improvement of the osseointegration of zirconia dental implants by HS-LEIS analysis

International Nuclear Information System (INIS)

Beekmans, H.; Breitenstein, D.; Brongersma, H.H.; Ridder, M. de; Tromp, Th.J.

2010-01-01

The use of sintered yttria stabilized zirconia dental implants is a recent development. After initial successes with these new implants a pattern of erratic results emerged. Reliable osseointegration would not always occur. High-sensitivity low energy ion scattering (HS-LEIS) is used to investigate both virgin and rejected implants. The surfaces of the implant are found to be covered with both an organic and inorganic contamination layer. Sterilization does not remove this contamination. Using LEIS as analytic tool a new cleaning process has been developed. Since this cleaning process is in use, the failure rate has dropped to a very low value.

Aroma volatiles obtained at harvest by HS-SPME/GC-MS and INDEX/MS-E-nose fingerprint discriminate climacteric behaviour in melon fruit.

Science.gov (United States)

Chaparro-Torres, Libia A; Bueso, María C; Fernández-Trujillo, Juan P

2016-05-01

Melon aroma volatiles were extracted at harvest from juice of a climacteric near-isogenic line (NIL) SC3-5-1 with two quantitative trait loci (QTLs) introgressed which produced climacteric behaviour and its non-climacteric parental (PS) using two methodologies of analysis: static headspace solid phase micro-extraction (HS-SPME) by gas chromatography-mass spectrometry (GC-MS) and inside needle dynamic extraction (INDEX) by MS-based electronic nose (MS-E-nose). Of the 137 volatiles compounds identified, most were found at significantly higher concentrations in SC3-5-1 than in PS in both seasons. These volatiles were mostly esters, alcohols, sulfur-derived esters and even some aldehydes and others. The number of variables with high correlation values was reduced by using correlation network analysis. Partial least squares-discriminant analysis (PLS-DA) achieved the correct classification of PS and SC3-5-1. The ions m/z 74, 91, 104, 105, 106 and 108, mainly volatile derivatives precursor phenylalanine, were the most discriminant in SC3-5-1 and PS. As many as 104 QTLs were mapped in season 1 and at least 78 QTLs in each season with an effect above the PS mean. GC-MS gave better discrimination than E-nose. Most of the QTLs that mapped in both seasons enhanced aroma volatiles associated with climacteric behaviour. © 2015 Society of Chemical Industry.

RoHS directive: restriction of the use of lead in electronic equipment

CERN Multimedia

2007-01-01

The European Restriction of Hazardous Substances (RoHS) directive, which entered into force on 1st July 2006, restricts the use of lead in the manufacture of electronic equipment. This has many consequences for the manufacture of printed circuit boards and assemblies, from their design and repair to the purchase of components. If you have any questions on this subject, we invite you to attend a training session which will be held at CERN from 1.30 to 5.30 p.m. on 21st June. All the details, including the course contents and instructions on how to register for it, can be found here. Please note that the session will be held in French.

RoHS directive: restriction of the use of lead in electronic equipment

CERN Multimedia

2007-01-01

The European Restriction of Hazardous Substances (RoHS) directive, which entered into force on 1st July 2006, restricts the use of lead in the manufacture of electronic equipment. This has many consequences for the manufacture of printed circuit boards and assemblies, from their design and repair to the purchase of components. If you have any questions on this subject, we invite you to attend a training session which will be held at CERN from 1.30 to 5.30 to p.m. on 21st June. All the details, including the course contents and instructions on how to register for it, can be found here Please note that the session will be held in French.

Study on synthesizing Mg/Al layered double hydroxides at different pHs

Directory of Open Access Journals (Sweden)

E Otgonjargal

2014-12-01

Full Text Available Mg/Al layered double hydroxide (LDH was successfully synthesized at different pHs values. The Mg/AL LDH was well characterized by X-Ray diffraction and Fourier transform infrared analysis. The morphology of the LDH was observed using Scanning electron microscopy with energy dispersive X-ray spectroscopy. The influence of pH values on the morphology of the Mg/Al LDHs were studied. The result showed that the well-synthesized Mg/Al LDHs could be obtained when the pH value was about 10.0 at room temperature.DOI: http://doi.dx.org/10.5564/mjc.v15i0.319 Mongolian Journal of Chemistry 15 (41, 2014, p36-39

Volatile-Compound Fingerprinting by Headspace-Gas-Chromatography Ion-Mobility Spectrometry (HS-GC-IMS) as a Benchtop Alternative to 1H NMR Profiling for Assessment of the Authenticity of Honey.

Science.gov (United States)

Gerhardt, Natalie; Birkenmeier, Markus; Schwolow, Sebastian; Rohn, Sascha; Weller, Philipp

2018-02-06

This work describes a simple approach for the untargeted profiling of volatile compounds for the authentication of the botanical origins of honey based on resolution-optimized HS-GC-IMS combined with optimized chemometric techniques, namely PCA, LDA, and kNN. A direct comparison of the PCA-LDA models between the HS-GC-IMS and 1 H NMR data demonstrated that HS-GC-IMS profiling could be used as a complementary tool to NMR-based profiling of honey samples. Whereas NMR profiling still requires comparatively precise sample preparation, pH adjustment in particular, HS-GC-IMS fingerprinting may be considered an alternative approach for a truly fully automatable, cost-efficient, and in particular highly sensitive method. It was demonstrated that all tested honey samples could be distinguished on the basis of their botanical origins. Loading plots revealed the volatile compounds responsible for the differences among the monofloral honeys. The HS-GC-IMS-based PCA-LDA model was composed of two linear functions of discrimination and 10 selected PCs that discriminated canola, acacia, and honeydew honeys with a predictive accuracy of 98.6%. Application of the LDA model to an external test set of 10 authentic honeys clearly proved the high predictive ability of the model by correctly classifying them into three variety groups with 100% correct classifications. The constructed model presents a simple and efficient method of analysis and may serve as a basis for the authentication of other food types.

Vertical distribution and structure of copepod (Arthropoda: Copepoda assemblages in two different seasons down to 1,200 m in the tropical Southwestern Atlantic

Directory of Open Access Journals (Sweden)

Cristina de Oliveira Dias

2018-05-01

Full Text Available ABSTRACT The vertical distribution of copepod assemblages, ascertained from the surface down to 1,200 m, was investigated during two sampling periods (rainy and dry seasons, at four depths, in the oligotrophic waters of the southwestern Atlantic Ocean. Total density, diversity, and richness differed among sampling depths. Copepod density decreased with depth in the two sampling periods, with a maximum at 1 m and a slight decrease at 800 m. Higher diversities were observed at 250 m and 1,200 m during the rainy season and at 1 m and 1,200 m during the dry season. The maximum number of species was found at 1,200 m during the rainy season and at 1 m during the dry season. Various copepod assemblages were delimited in the water column in the two sampling periods. The deeper copepod assemblages occupied a wider range of depths. Salinity and temperature influenced the structure of copepod assemblages and reflected the hydrographic characteristics of the water masses in the region. Candacia pachydactyla (Dana, 1849, Scolecithrix danae (Lubbock, 1856, and Agetus limbatus (Brady, 1883 were the indicator species found at 1 m. The effects of different environmental factors on the copepod assemblages suggest that these consortia occupy distinct niches in the ocean.

Tensile properties of commercially pure vanadium from room temperature to 1200 degree C

International Nuclear Information System (INIS)

Henshall, G.A.; Torres, S.G.

1993-12-01

The tensile properties of vanadium are sensitive to interstitial impurity content, on grain size and strain rate. Thus, it is problematic to use published tensile data for materials potentially varying in these quantities. This investigation was undertaken to fully characterize the tensile properties of the commercially pure vanadium used at Lawrence Livermore. Both sheet and rod stock were tested in vacuum from ambient temperature to 1200C at strain rates 6.67 x 10 -5 to 6.67 x 10 -2 s -1 . The results of these experiments show that vanadium behaves in a manner typical of many bcc metals containing interstitial impurities. Local peaks in yield stress and ultimate tensile stress vs temperature curves are observed at intermediate temperatures. Serrated yielding also is observed in some temperature ranges. Changes in strain rate within the quasi-static regime have a relatively small, predictable effect. The rod and sheet stock have similar properties, except that the lower yield stress of the rod is less than that of the sheet over most of the temperature range studied. No plateau in yield strength vs temperature curve was observed for the rod. In both forms, and for all temperatures, vanadium is ductile. The elongation to failure reaches a minimum of approximately 35% at a temperature of 500C and a maximum of approximately 140% at 1200C

1200 nt rat liver mRNA identified by differential hybridization exhibits coordinate regulation with 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase

International Nuclear Information System (INIS)

Tanaka, R.D.; Clarke, C.F.; Fogelman, A.M.; Edwards, P.A.

1986-01-01

Differential hybridization has been used to identify genes in rat liver that encode transcripts which are increased by the drugs cholestyramine and mevinolin and are decreased by dietary cholesterol. This approach should prove useful in isolating and identifying coordinately regulated genes involved in the isoprene biosynthetic pathway. Rat liver poly (A) + RNA was isolated from animals fed diets supplemented with either cholestyramine and mevinolin or with cholesterol. Radiolabeled cDNAs generated from these two RNA preparations were used to screen a rat cDNAs library. A preliminary screen of 10,000 recombinants has led to the identification of a clone with an insert of 1200 bp that hybridizes to a mRNA species of about 1200 nt. The level of this RNA species in rat liver is elevated by the drugs cholestyramine and mevinolin and is decreased by cholesterol feeding. This RNA species is also decreased by mevalonate administration to rats. The regulation of this 1200 nt mRNA species mirrors that of HMG CoA reductase and HMG CoA synthase. It seems very likely that this 1200 nt mRNA encodes a polypeptide which is involved in the isoprene biosynthetic pathway

Association Between Free Fatty Acid (FFA and Insulin Resistance: The Role of Inflammation (Adiponectin and high sensivity C-reactive Protein/hs-CRP and Stress Oxidative (Superoxide Dismutase/SOD in Obese Non-Diabetic Individual

Directory of Open Access Journals (Sweden)

Indriyanti Rafi Sukmawati

2009-12-01

Full Text Available BACKGROUND: Obesity is highly related to insulin resistance, therefore, the increased number of obesity is followed by the increased prevalence of type 2 Diabetes Melitus. Obesity is associated with increased of reactive oxygen species (ROS in muscle, liver and endothelial cells. The increase of ROS would lead to insulin resistance (IR and increased pro-inflammatory protein. FFA plays an important role in IR by inhibiting muscle glucose transport and oxidation via effects on serine/threonine phosphorylation of IRS-1. The aim of this study was discover the existence of SOD, hs-CRP and and adiponectin levels towards the occurrence of insulin resistance which was caused by elevated level of FFA and to discover the interaction between SOD, hs-CRP and adiponectin in non diabetic obese adult male. METHODS: This was observational study with cross sectional design. There were 65 obese male non diabetic subjects and 45 non obese male non diabetic subjects who met the criteria. In this study, measurements were done on body mass index (BMI, fasting glucose, insulin, adiponectin, hs-CRP and SOD. Obese was defined as BMI >25 kg/m2, normal weight was defined as BMI 18.5-23 kh/m2 and Insulin Resistance was defined as HOMA-IR >1. RESULTS: This study showed that Hypoadiponectinemia condition, decreased SOD level and high level of hs-CRP is associated with insulin resistance in obese non diabetic subject. Adiponectin and SOD were correlated negatively with insulin resistance in obese non diabetic (Adiponectin, r=-0.455, p<0.001; SOD, r=-0.262, p=0.003, hs-CRP was positively correlated with insulin resistance in obese non diabetic (r=0.592, p<0.001. FFA levels was increased in obese insulin resistance compared with non obese non insulin resistance. The Odds Ratio of Adiponectin, hs-CRP and SOD in this study was analyzed by logistic binary. The OR for SOD 3.6 (p=0.001, hs-CRP 9.1 (p<0.001 and Adiponectin 7.2 (p<0.001. CONCLUSIONS: This study suggested that FFA

Photoreduction of Terrigenous Fe-Humic Substances Leads to Bioavailable Iron in Oceans.

Science.gov (United States)

Blazevic, Amir; Orlowska, Ewelina; Kandioller, Wolfgang; Jirsa, Franz; Keppler, Bernhard K; Tafili-Kryeziu, Myrvete; Linert, Wolfgang; Krachler, Rudolf F; Krachler, Regina; Rompel, Annette

2016-05-23

Humic substances (HS) are important iron chelators responsible for the transport of iron from freshwater systems to the open sea, where iron is essential for marine organisms. Evidence suggests that iron complexed to HS comprises the bulk of the iron ligand pool in near-coastal waters and shelf seas. River-derived HS have been investigated to study their transport to, and dwell in oceanic waters. A library of iron model compounds and river-derived Fe-HS samples were probed in a combined X-ray absorption spectroscopy (XAS) and valence-to-core X-ray emission spectroscopy (VtC-XES) study at the Fe K-edge. The analyses performed revealed that iron complexation in HS samples is only dependent on oxygen-containing HS functional groups, such as carboxyl and phenol. The photoreduction mechanism of Fe III -HS in oceanic conditions into bioavailable aquatic Fe II forms, highlights the importance of river-derived HS as an iron source for marine organisms. Consequently, such mechanisms are a vital component of the upper-ocean iron biogeochemistry cycle.

Metafore spaziali della conoscenza di sé nei dialoghi di H.S. Skovoroda. Fonti patristiche

Directory of Open Access Journals (Sweden)

Maria Grazia Bartolini

2009-01-01

Full Text Available Spatial Metaphors for Self-knowledge in the dialogues of H.S Skovoroda. Patristic Sources In this paper the Author analyzes some spatial metaphors informing H.S. Skovoroda’s representation of self-knowledge as a dynamic process. Knowledge of the true self – which is one of the recurring themes in the Ukrainian philosopher’s oeuvre – is often portrayed as tension between open (e.g. streets, markets, the sky, the planets… and closed spaces (e.g. the house, the room, with the ‘home’ being a symbol for the soul. As man seeking communion with God has to abandon what lies outside his inner self, the authentic gnoseological process is represented as an introverted movement through symbolic space. Broadly speaking, this very opposition harks back to Philo of Alexandria and Patristic thought, whose anti-physicist stance shaped their rejection of the phenomenological world as an ‘interior vs. exterior’ contrast. Furthermore, the broader biblical context in which those spatial terms appear is also taken into account, while demonstrating Skovoroda’s partial dependence on Patristic models. The fi nal part of the paper argues that the scheme underlying Neo-platonic emanation – which is itself to be considered as an oscillation between unity and dispersion – appears to be a symbolic model for Skovoroda’s spatial representation of self-knowledge.

Changes of serum tumor markers, immunoglobulins, TNF-α and hs-CRP levels in patients with breast cancer and its clinical significance

Institute of Scientific and Technical Information of China (English)

Jian-Gang Dai; Yong-Feng Wu; Mei Li

2017-01-01

Objective: To study the serum tumor markers, immunoglobulin, TNF-α and hs-CRP in breast cancer in different pathological stages of the concentration, and to analyze the clinical significance of early diagnosis of breast cancer. Methods: A total of 130 patients with breast cancer were divided into stage I, II, III and IV according to clinical pathology. In addition, 40 patients with benign breast disease and 35 healthy subjects were selected as benign breast disease group and control group. Serum tumor markers, immunoglobulins, TNF-αand hs-CRP concentrations were measured and compared of all subjects. Results: There were no significant difference in serum tumor markers, immunoglobulin and inflammatory factors between the control group and the benign breast cancer group. The level of serum tumor markers in breast cancer group was significantly higher than that in control group and benign breast cancer group. The levels of serum CA125, CA153 and CEA were gradually increased with the severity enhancing from stage I and IV of breast cancer, and he difference was statistically significant. The level of serum immunoglobulin in breast cancer group was significantly higher than that in control group and benign breast cancer group. The levels of serum IgG and IgM increased gradually severity enhancing from stage I and IV of breast cancer, and the difference was statistically significant. The level of serum TNF-α and hs-CRP in serum of breast cancer group was significantly higher than that of control group and benign breast cancer group. The serum levels of TNF-α and hs-CRP increased gradually with severity enhancing from stage I and IV of breast cancer, and the difference was statistically significant. Conclusion: The level of serum tumor markers in breast cancer patients is increasing. Humoral and inflammatory responses are activated to varying degrees and increase with the aggregation of disease. They may involve regulating the occurrence and metastasis of breast

Characterization of the Solutol® HS15/water phase diagram and the impact of the Δ9-tetrahydrocannabinol solubilization.

Science.gov (United States)

Murgia, Sergio; Fadda, Paolo; Colafemmina, Giuseppe; Angelico, Ruggero; Corrado, Luigi; Lazzari, Paolo; Monduzzi, Maura; Palazzo, Gerardo

2013-01-15

Here, the phase behavior of the commercial non-ionic surfactant Solutol® HS15 in water was investigated. The focus was on the evolution of the system nanostructure at low water content. Particularly, it was demonstrated that spherical micelles found in dilute surfactant solutions coalesce at a surfactant volume fraction close to 0.5. As consequence, a heterogeneous pseudo-binary mixture occurs. No liquid crystalline phases were detected even at the highest HS15 concentrations in water. Alteration of the micellar morphology induced by the addition of Δ(9)-tetrahydrocannabinol to the surfactant/water binary system was also investigated. It was found that the cannabinoid molecules become entrapped within the surfactant hydrophobic tails, thus increasing the surfactant effective packing parameter and inducing a radical change of the micelle shape. At sufficiently low water content (18-35 wt.%), such alteration of the interfacial packing results in a lamellar organization of the surfactant molecules. Copyright © 2012 Elsevier Inc. All rights reserved.

Design and study of some novel ibuprofen derivatives with potential nootropic and neuroprotective properties.

Science.gov (United States)

Siskou, Ioanna C; Rekka, Eleni A; Kourounakis, Angeliki P; Chrysselis, Michael C; Tsiakitzis, Kariofyllis; Kourounakis, Panos N

2007-01-15

Six novel ibuprofen derivatives and related structures, incorporating a proline moiety and designed for neurodegenerative disorders, are studied. They possess anti-inflammatory properties and three of them inhibited lipoxygenase. One compound was found to inhibit cyclooxygenase (COX)-2 production in spleenocytes from arthritic rats. The HS-containing compounds are potent antioxidants and one of them protected against glutathione loss after cerebral ischemia/reperfusion. They demonstrated lipid-lowering ability and seem to acquire low gastrointestinal toxicity. Acetylcholinesterase inhibitory activity, found in two of these compounds, may be an asset to their actions.

CoCoRaHS (The Community Collaborative Rain, Hail and Snow Network): Analysis of Participant Survey Data to Uncover Learning through Participation

Science.gov (United States)

Holzer, M. A.; Zimmerman, T.; Doesken, N. J.; Reges, H. W.; Newman, N.; Turner, J.; Schwalbe, Z.

2010-12-01

CoCoRaHS (The Community Collaborative Rain, Hail and Snow network) is based out of Fort Collins Colorado and is an extremely successful citizen science project with over 15,000 volunteers collecting valuable precipitation data. Forecasters and scientists use data from this dense network to illuminate and illustrate the high small-scale variability of precipitation across the nation. This presentation will discuss the results of a survey of CoCoRaHS participants as related to 1) citizen scientists’ motivation and learning; 2) the challenges of identifying how people learn science in citizen science projects; and 3) a potential research-based framework for how people learn through engaging in the data collection within in a citizen science project. A comprehensive survey of 14,500 CoCoRaHS observers was recently conducted to uncover participant perceptions of numerous aspects of the CoCoRaHS program, including its goal of increasing climate literacy. The survey yielded a response rate of over 50%, and included measures of motivation, engagement and learning. In relationship to motivation and learning, the survey revealed that most (57.1%) observers would make precipitation observations regardless of being a CoCoRaHS volunteer, therefore their motivation is related to their inherent level of interest in weather. Others are motivated by their desire to learn more about weather and climate, they want to contribute to a scientific project, they think its fun, and/or it provides a sense of community. Because so many respondents already had knowledge and interest in weather and climate, identifying how and what people learn through participating was a challenge. However, the narrow project focus of collecting and reporting of local precipitation assisted in identifying aspects of learning. For instance, most (46.4%) observers said they increased their knowledge about the local variability in precipitation even though they had been collecting precipitation data for many

Discussion on the 3D visualizing of 1:200 000 geological map

Science.gov (United States)

Wang, Xiaopeng

2018-01-01

Using United States National Aeronautics and Space Administration Shuttle Radar Topography Mission (SRTM) terrain data as digital elevation model (DEM), overlap scanned 1:200 000 scale geological map, program using Direct 3D of Microsoft with C# computer language, the author realized the three-dimensional visualization of the standard division geological map. User can inspect the regional geology content with arbitrary angle, rotating, roaming, and can examining the strata synthetical histogram, map section and legend at any moment. This will provide an intuitionistic analyzing tool for the geological practitioner to do structural analysis with the assistant of landform, dispose field exploration route etc.

Shaping of the axial power density distribution in the core to minimize the vapor volume fraction at the outlet of the VVER-1200 fuel assemblies

Energy Technology Data Exchange (ETDEWEB)

Savander, V. I.; Shumskiy, B. E., E-mail: borisshumskij@yandex.ru [National Research Nuclear University MEPhI (Russian Federation); Pinegin, A. A. [National Research Center Kurchatov Institute (Russian Federation)

2016-12-15

The possibility of decreasing the vapor fraction at the VVER-1200 fuel assembly outlet by shaping the axial power density field is considered. The power density field was shaped by axial redistribution of the concentration of the burnable gadolinium poison in the Gd-containing fuel rods. The mathematical modeling of the VVER-1200 core was performed using the NOSTRA computer code.

Modeling of corrosion product migration in the secondary circuit of nuclear power plants with WWER-1200

Science.gov (United States)

Kritskii, V. G.; Berezina, I. G.; Gavrilov, A. V.; Motkova, E. A.; Zelenina, E. V.; Prokhorov, N. A.; Gorbatenko, S. P.; Tsitser, A. A.

2016-04-01

Models of corrosion and mass transfer of corrosion products in the pipes of the condensate-feeding and steam paths of the secondary circuit of NPPs with WWER-1200 are presented. The mass transfer and distribution of corrosion products over the currents of the working medium of the secondary circuit were calculated using the physicochemical model of mass transfer of corrosion products in which the secondary circuit is regarded as a cyclic system consisting of a number of interrelated elements. The circuit was divided into calculated regions in which the change in the parameters (flow rate, temperature, and pressure) was traced and the rates of corrosion and corrosion products entrainment, high-temperature pH, and iron concentration were calculated. The models were verified according to the results of chemical analyses at Kalinin NPP and iron corrosion product concentrations in the feed water at different NPPs depending on pH at 25°C (pH25) for service times τ ≥ 5000 h. The calculated pH values at a coolant temperature t (pH t ) in the secondary circuit of NPPs with WWER-1200 were presented. The calculation of the distribution of pH t and ethanolamine and ammonia concentrations over the condensate feed (CFC) and steam circuits is given. The models are designed for developing the calculation codes. The project solutions of ATOMPROEKT satisfy the safety and reliability requirements for power plants with WWER-1200. The calculated corrosion and corrosion product mass transfer parameters showed that the model allows the designer to choose between the increase of the correcting reagent concentration, the use of steel with higher chromium contents, and intermittent washing of the steam generator from sediments as the best solution for definite regions of the circuit.

Study on safety analysis of VVER-1200/V491 in scenario of Loss of Coolant Accidents along with partly failure of ECCS using RELAP5 code

International Nuclear Information System (INIS)

Hoang Minh Giang; Ha Thi Anh Dao; Hoang Tan Hung; Bui Thi Hoa; Nguyen Thi Tu Oanh; Dinh Anh Tuan; Pham Tuan Nam

2017-01-01

The advanced VVER-1200/V491 reactor designed with passive safety systems to deal with design extension conditions is primarily selected as priority candidate for Ninh Thuan 1 nuclear power plant project. So that, in order to enhance competence of nuclear safety and toward participation on review Safety Analysis Report (SAR) of Ninh Thuan nuclear Power project the study on safety analysis of VVER-1200/V491 in scenario of Loss of Coolant Accidents along with partly failure of ECCS is implemented. As requirement of the study, the input deck file of VVER-1200/V491 for RELAP5 and analysis report for some special case of LOCAs along with partly failure of ECCS are issued. (author)

A Nampt inhibitor FK866 mimics vitamin B3 deficiency by causing senescence of human fibroblastic Hs68 cells via attenuation of NAD(+)-SIRT1 signaling.

Science.gov (United States)

Song, Tuzz-Ying; Yeh, Shu-Lan; Hu, Miao-Lin; Chen, Mei-Yau; Yang, Nae-Cherng

2015-12-01

Vitamin B3 (niacin) deficiency can cause pellagra with symptoms of dermatitis, diarrhea and dementia. However, it is unclear whether the vitamin B3 deficiency causes human aging. FK866 (a Nampt inhibitor) can reduce intracellular NAD(+) level and induce senescence of human Hs68 cells. However, the mechanisms underlying FK866-induced senescence of Hs68 cells are unclear. In this study, we used FK866 to mimic the effects of vitamin B3 deficiency to reduce the NAD(+) level and investigated the mechanisms of FK866-induced senescence of Hs68 cells. We hypothesized that FK866 induced the senescence of Hs68 cells via an attenuation of NAD(+)-silent information regulator T1 (SIRT1) signaling. We found that FK866 induced cell senescence and diminished cellular NAD(+) levels and SIRT1 activity (detected by acetylation of p53), and these effects were dramatically antagonized by co-treatment with nicotinic acid, nicotinamide, or NAD(+). In contrast, the protein expression of SIRT1, AMP-activated protein kinase, mammalian target of rapamycin, and nicotinamide phosphoribosyltransferase (Nampt) was not affected by FK866. In addition, the role of GSH in the FK866-induced cells senescence may be limited, as N-acetylcysteine did not antagonize FK866-induced cell senescence. These results suggest that FK866 induces cell senescence via attenuation of NAD(+)-SIRT1 signaling. The effects of vitamin B3 deficiency on human aging warrant further investigation.

Intake of traditional Inuit diet vary in parallel with inflammation as estimated from YKL-40 and hsCRP in Inuit and non-Inuit in Greenland.

Science.gov (United States)

Schæbel, L H; Vestergaard, H; Laurberg, P; Rathcke, C N; Andersen, S

2013-06-01

Chronic low-grade inflammation is involved in the initiation and progression of atherosclerosis and ischemic heart disease. This was rare in pre-western Inuit who lived on a diet that consisted mainly of marine mammals rich in n-3 fatty acids. To assess the association between biomarkers of inflammation and the intake of traditional Inuit diet in addition to Inuit ethnicity. YKL-40 and hsCRP were measured in serum from 535 Inuit and non-Inuit living in the capital city Nuuk in West Greenland or in the main town or a settlement in rural East Greenland. Dietary habits were assessed by an interview-based food frequency questionnaire. The participation rate was 95%. YKL-40 was higher in Inuit than in non-Inuit (p Inuit with a higher intake of traditional Inuit diet (p Inuit from rural compared to urban areas (p Inuit had higher hsCRP compared to non-Inuit (p = 0.003) and hsCRP increased in parallel with intake of traditional Inuit foods (p Inuit (p = 0.004). YKL-40 and hsCRP increased with higher intakes of traditional Inuit diet after adjusting for ethnicity, gender, age, smoking, alcohol intake and BMI. Biomarkers of inflammation vary in parallel with the intake of traditional Inuit diet. A diet based on marine mammals from the Arctic does not reduce inflammatory activity and it may be speculated that markers of inflammation reflect the disease rather than the cause of the disease. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Factors Influencing the Antifolate Activity of Synthetic Tea-Derived Catechins

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José Neptuno Rodríguez-López

2013-07-01

Full Text Available Novel tea catechin derivatives have been synthesized, and a structure-activity study, related to the capacity of these and other polyphenols to bind dihydrofolate reductase (DHFR, has been performed. The data showed an effective binding between all molecules and the free enzyme, and the dissociation constants of the synthetic compounds and of the natural analogues were on the same order. Polyphenols with a catechin configuration were better DHFR inhibitors than those with an epicatechin configuration. Antiproliferative activity was also studied in cultured tumour cells, and the data showed that the activity of the novel derivatives was higher in catechin isomers. Derivatives with a hydroxyl group para on the ester-bonded gallate moiety presented a high in vitro binding to DHFR, but exhibited transport problems in cell culture due to ionization at physiologic pHs. The impact of the binding of catechins to serum albumin on their biological activity was also evaluated. The information provided in this study could be important for the design of novel medicinal active compounds derived from tea catechins. The data suggest that changes in their structure to avoid serum albumin interactions and to facilitate plasmatic membrane transport are essential for the intracellular functions of catechins.

A critical evaluation of the Beckman Coulter Access hsTnI: Analytical performance, reference interval and concordance.

Science.gov (United States)

Pretorius, Carel J; Tate, Jillian R; Wilgen, Urs; Cullen, Louise; Ungerer, Jacobus P J

2018-05-01

We investigated the analytical performance, outlier rate, carryover and reference interval of the Beckman Coulter Access hsTnI in detail and compared it with historical and other commercial assays. We compared the imprecision, detection capability, analytical sensitivity, outlier rate and carryover against two previous Access AccuTnI assay versions. We established the reference interval with stored samples from a previous study and compared the concordances and variances with the Access AccuTnI+3 as well as with two commercial assays. The Access hsTnI had excellent analytical sensitivity with the calibration slope 5.6 times steeper than the Access AccuTnI+3. The detection capability was markedly improved with the SD of the blank 0.18-0.20 ng/L, LoB 0.29-0.33 ng/L and LoD 0.58-0.69 ng/L. All the reference interval samples had a result above the LoB value. At a mean concentration of 2.83 ng/L the SD was 0.28 ng/L (CV 9.8%). Carryover (0.005%) and outlier (0.046%) rates were similar to the Access AccuTnI+3. The combined male and female 99th percentile reference interval was 18.2 ng/L (90% CI 13.2-21.1 ng/L). Concordance amongst the assays was poor with only 16.7%, 19.6% and 15.2% of samples identified by all 4 assays as above the 99th, 97.5th and 95th percentiles. Analytical imprecision was a minor contributor to the observed variances between assays. The Beckman Coulter Access hsTnI assay has excellent analytical sensitivity and precision characteristics close to zero. This allows cTnI measurement in all healthy individuals and the capability to identify numerically small differences between serial samples as statistically significant. Concordance in healthy individuals remains poor amongst assays. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

Key features of MIR.1200 (AES-2006) design and current stage of Leningrad NPP-2 construction

International Nuclear Information System (INIS)

Ivkov, Igor

2010-01-01

MIR.1200/AES-2006 is an abbreviated name of the evolving NPP design developed on the basis of the VVER-1000 Russian design with gross operation life of 480 reactor-years. This design is being implemented in four Units of Leningrad NPP-2 (LNPP-2. The AES-91/99 was used as reference during development of the AES-2006 design for LNPP-2; this design was implemented in two Units of Tianwan NPP (China). The main technical features of the MIR.1200/AES-2006 design include a double containment, four trains of active safety systems (4x100%, 4x50%), and special engineering measures for BDBA management (core catcher, H2 PARs, PHRS) based mainly on passive principles. The containment is described in detail, the main features in comparison with the reference NPP are outlined, the design layout principles are highlighted, the safety system structure and parameters are described. Attention is paid to the BDBA management system, hydrogen removal system, core catcher, and PHRS-SG and C-PHRS. (P.A.)

The Predictive Role of Tooth Extractions, Oral Infections, and hs-C-Reactive Protein for Mortality in Individuals with and without Diabetes: A Prospective Cohort Study of a 12 1/2-Year Follow-Up

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Lise Lund Håheim

2017-01-01

Full Text Available The predictive role of high-sensitivity C-reactive protein (hs-CRP, number of tooth extractions, and oral infections for mortality in people with and without diabetes is unclear. This prospective cohort study is a 12 1/2-year follow-up of the Oslo II study, a health survey in 2000. In all, 12,764 men were invited. Health information was retrieved from 6434 elderly men through questionnaire information, serum measurements, and anthropometric and blood pressure measurements. Diabetes was reported by 425 men. Distinct differences were observed in baseline characteristics in individuals with and without diabetes. In the diabetes group, age and hs-CRP were statistically significant whereas in the nondiabetes group, age, hs-CRP, number of tooth extractions, tooth extractions for infections and oral infections combined, nonfasting glucose, systolic blood pressure, total cholesterol, regular alcohol drinking, daily smoking, and level of education were independent risk factors. The number of tooth extractions <5 was inversely related whereas more extractions increased the risk. Multivariate analyses showed that hs-CRP was a significant predictor in persons with diabetes and tooth extractions and oral infections combined; the number of teeth extracted and hs-CRP were for persons without diabetes. Infection and inflammation were associated with mortality in individuals both with and without diabetes.

Swertianlarin, an Herbal Agent Derived from Swertia mussotii Franch, Attenuates Liver Injury, Inflammation, and Cholestasis in Common Bile Duct-Ligated Rats

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Liangjun Zhang

2015-01-01

Full Text Available Swertianlarin is an herbal agent abundantly distributed in Swertia mussotii Franch, a Chinese traditional herb used for treatment of jaundice. To study the therapeutic effect of swertianlarin on cholestasis, liver injury, serum proinflammatory cytokines, and bile salt concentrations were measured by comparing rats treated with swertianlarin 100 mg/kg/d or saline for 3, 7, or 14 days after bile duct ligation (BDL. Serum alanine aminotransferase (ATL and aspartate aminotransferase (AST levels were significantly decreased in BDL rats treated with swertianlarin for 14 days (P<0.05. The reduced liver injury in BDL rats by swertianlarin treatment for 14 days was further confirmed by liver histopathology. Levels of serum tumor necrosis factor alpha (TNFα were decreased by swertianlarin in BDL rats for 3 and 7 days (P<0.05. Moreover, reductions in serum interleukins IL-1β and IL-6 levels were also observed in BDL rats treated with swertianlarin (P<0.05. In addition, most of serum toxic bile salt concentrations (e.g., chenodeoxycholic acid (CDCA and deoxycholic acid (DCA in cholestatic rats were decreased by swertianlarin (P<0.05. In conclusion, the data suggest that swertianlarin derived from Swertia mussotii Franch attenuates liver injury, inflammation, and cholestasis in bile duct-ligated rats.

40 CFR 180.1200 - Pseudomonas fluorescens strain PRA-25; temporary exemption from the requirement of a tolerance.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pseudomonas fluorescens strain PRA-25... RESIDUES IN FOOD Exemptions From Tolerances § 180.1200 Pseudomonas fluorescens strain PRA-25; temporary... established for residues of the microbial pesticide, pseudomonas fluorescens strain PRA-25 when used on peas...

ROCKY EXTRASOLAR PLANETARY COMPOSITIONS DERIVED FROM EXTERNALLY POLLUTED WHITE DWARFS

International Nuclear Information System (INIS)

Klein, B.; Jura, M.; Zuckerman, B.; Koester, D.

2011-01-01

We report Keck High Resolution Echelle Spectrometer data and model atmosphere analysis of two helium-dominated white dwarfs, PG1225–079 and HS2253+8023, whose heavy pollutions most likely derive from the accretion of terrestrial-type planet(esimal)s. For each system, the minimum accreted mass is ∼10 22 g, that of a large asteroid. In PG1225–079, Mg, Cr, Mn, Fe, and Ni have abundance ratios similar to bulk Earth values, while we measure four refractory elements, Ca, Sc, Ti, and V, all at a factor of ∼2-3 higher abundance than in the bulk Earth. For HS2253+8023 the swallowed material was compositionally similar to bulk Earth in being more than 85% by mass in the major element species, O, Mg, Si, and Fe, and with abundances in the distinctive proportions of mineral oxides—compelling evidence for an origin in a rocky parent body. Including previous studies we now know of four heavily polluted white dwarfs where the measured oxygen and hydrogen are consistent with the view that the parents' bodies formed with little ice, interior to any snow line in their nebular environments. The growing handful of polluted white dwarf systems with comprehensive abundance measurements form a baseline for characterizing rocky exoplanet compositions that can be compared with bulk Earth.

Correction to h-zr.27t: the H in Zirc Hydride S(α,β) Data at 1200K in the ENDF71SaB Library

Energy Technology Data Exchange (ETDEWEB)

Parsons, Donald Kent [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-01-31

A problem was reported in the S(α,β) data for H in Zirc Hydride at 1200 K. The particular S(α,β) data in question is denominated as h-zr.27t (or alternatively, h/zr.27t). Plots of the data and an explanation of the problem are given. A new S(α,β) file denominated as h-zr.28t is now the official data at 1200 K.

A Restructuring of the HS Package for the MIDAS Computer Code

International Nuclear Information System (INIS)

Park, S. H.; Kim, K. R.; Kim, D. H.; Cho, S. W.

2005-01-01

As one of the processes for a localized severe accident analysis code, KAERI is developing a severe accident code MIDAS, based on MELCOR. The existing data saving method uses pointer variables for a fix-sized storage management, and it deteriorates the readability, maintainability and portability of the code. But new features in FORTRAN90 such as a dynamic allocation have been used for the restructuring. The restructuring of the data saving and transferring method of the existing code makes it easy to understand the code. Before an entire restructuring of the code, a restructuring template for a simple package was developed and tested. The target for the restructuring was the HS package which is responsible for calculation the heat conduction within an intact, solid structure and energy transfer across its boundary surfaces into control volumes. The verification was done through comparing the results before and after the restructuring

HS-SPME-GC-MS Analysis of onion (Allium cepa L. and shallot (Allium ascalonicum L.

Directory of Open Access Journals (Sweden)

D’Auria, M.

2017-06-01

Full Text Available The volatile organic compounds of onion and shallot were determined via HS-SPME-GC-MS. The main components were dipropyldisulphide and allylpropyldisulphide. Thiopropanal S-oxide were detected only in onion volatiles. In shallot is interesting the presence of 2-methyl-2-pentenal, a compound with an intense fruity aroma, that can characterize the different aroma between onion and shallot. The SPME-GC-MS analysis of shallot after absorption on the SPME fiber at 50°C showed the presence of new compounds, whose structures have been discussed.

Administration of NaHS attenuates footshock-induced pathologies and emotional and cognitive dysfunction in triple transgenic Alzheimer’s mice

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Hei-Jen eHuang

2015-11-01

Full Text Available Alzheimer's disease (AD is characterized by progressive cognitive decline and neuropsychiatric symptoms. Increasing evidence indicates that environmental risk factors in young adults may accelerate cognitive loss in AD and that hydrogen sulfide (H2S may represent an innovative treatment to slow the progression of AD. Therefore, the aim of this study was to evaluate the effects of NaHS, an H2S donor, in a triple transgenic AD mouse model (3×Tg-AD under footshock with situational reminders (SRs. Inescapable footshock with SRs induced anxiety and cognitive dysfunction as well as a decrease in the levels of plasma H2S and GSH and an increase in IL-6 levels in 3×Tg-AD mice. Under footshock with SR stimulus, amyloid deposition, tau protein hyperphosphorylation, and microgliosis were highly increased in the stress-responsive brain structures, including the hippocampus and amygdala, of the AD mice. Oxidative stress, inflammatory response, and β-site APP cleaving enzyme 1 (BACE1 levels were also increased, and the level of inactivated glycogen synthase kinase-3β (GSK3β (pSer9 was decreased in the hippocampi of AD mice subjected to footshock with SRs. Furthermore, the numbers of cholinergic neurons in the medial septum/diagonal band of Broca (MS/DB and noradrenergic neurons in the locus coeruleus (LC were also decreased in the 3×Tg-AD mice under footshock with SRs. These biochemical hallmarks and pathological presentations were all alleviated by the semi-acute administration of NaHS in the AD mice. Together, these findings suggest that footshock with SRs induces the impairment of spatial cognition and emotion, which involve pathological changes in the peripheral and central systems, including the hippocampus, MS/DB, LC, and BLA, and that the administration of NaHS may be a candidate strategy to ameliorate the progression of neurodegeneration.

The effect of mud pack therapy on serum YKL-40 and hsCRP levels in patients with knee osteoarthritis.

Science.gov (United States)

Güngen, Gonca; Ardic, Fusun; Fındıkoğlu, Gülin; Rota, Simin

2012-05-01

The aim of this study was to evaluate the efficacy of treatment with mud pack in knee osteoarthritis (OA) and to determine whether mud pack effects serum levels of YKL-40 and high-sensitivity C-reactive protein (hsCRP) which are reported to be biological markers for articular damage or inflammation in patients with OA. Forty-four patients with the diagnosis of knee OA assigned into two groups were treated with local natural mineral-rich mud pack or hot pack. Treatments were applied for 6 days a week for 2 weeks as a total of 12 sessions. Patients were assessed at baseline, post-treatment, and 3 months after the treatment. VAS, range of motion, 15-m walking time, WOMAC index, Nottingham Health Profile, serum YKL-40, and hsCRP levels were the outcome measures. Pain intensity and joint stiffness decreased in both groups at all follow-ups. Physical activity status was found to persist for 3 months after treatment only in mud pack group. Serum mean YKL-40 and hsCRP levels of the patients were higher compared to healthy control group. Serum YKL-40 level increased significantly only in hot pack group 3 months after the treatment (P 0.05). Mud pack and hot pack therapy were both demonstrated to be effective in symptomatic treatment of knee OA until the end of the 2-week treatment period, whereas only mud pack therapy was shown to be effective in functional status over time. In the hot pack group, increased serum YKL-40 level 3 months after the treatment might indicate persistence of cartilage degradation. Maintenance of YKL-40 level in mud pack therapy seems to slow down the progression of knee OA.

The transition of mouse pluripotent stem cells from the naïve to the primed state requires Fas signaling through 3-O sulfated heparan sulfate structures recognized by the HS4C3 antibody

International Nuclear Information System (INIS)

Hirano, Kazumi; Van Kuppevelt, Toin H.; Nishihara, Shoko

2013-01-01

Highlights: ► Fas transcript increases during the transition from the naïve to the primed state. ► 3OST-5 transcript, the HS4C3 epitope synthesis gene, increases during the transition. ► Fas signaling regulates the transition from the naïve to the primed state. ► HS4C3-binding epitope regulates the transition from the naïve to the primed state. ► Fas signaling is regulated by the HS4C3 epitope during the transition. -- Abstract: The characteristics of pluripotent embryonic stem cells of human and mouse are different. The properties of human embryonic stem cells (hESCs) are similar to those of mouse epiblast stem cells (mEpiSCs), which are in a later developmental pluripotency state, the so-called “primed state” compared to mouse embryonic stem cells (mESCs) which are in a naïve state. As a result of the properties of the primed state, hESCs proliferate slowly, cannot survive as single cells, and can only be transfected with genes at low efficiency. Generating hESCs in the naïve state is necessary to overcome these problems and allow their application in regenerative medicine. Therefore, clarifying the mechanism of the transition between the naïve and primed states in pluripotent stem cells is important for the establishment of stable methods of generating naïve state hESCs. However, the signaling pathways which contribute to the transition between the naïve and primed states are still unclear. In this study, we carried out induction from mESCs to mEpiSC-like cells (mEpiSCLCs), and observed an increase in the activation of Fas signaling during the induction. The expression of Fgf5, an epiblast marker, was diminished by inhibition of Fas signaling using the caspase-8 and -3 blocking peptides, IETD and DEVD, respectively. Furthermore, during the induction, we observed increased expression of 3-O sulfated heparan sulfate (HS) structures synthesized by HS 3-O-sulfotransferase (3OST), which are recognized by the HS4C3 antibody (HS4C3-binding epitope

The transition of mouse pluripotent stem cells from the naïve to the primed state requires Fas signaling through 3-O sulfated heparan sulfate structures recognized by the HS4C3 antibody

Energy Technology Data Exchange (ETDEWEB)

Hirano, Kazumi [Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236 Tangi-cho, Hachioji, Tokyo 192-8577 (Japan); Van Kuppevelt, Toin H. [Department of Biochemistry, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, 280 P.O. Box 9101, 6500 HB Nijmegen (Netherlands); Nishihara, Shoko, E-mail: shoko@soka.ac.jp [Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236 Tangi-cho, Hachioji, Tokyo 192-8577 (Japan)

2013-01-18

Highlights: ► Fas transcript increases during the transition from the naïve to the primed state. ► 3OST-5 transcript, the HS4C3 epitope synthesis gene, increases during the transition. ► Fas signaling regulates the transition from the naïve to the primed state. ► HS4C3-binding epitope regulates the transition from the naïve to the primed state. ► Fas signaling is regulated by the HS4C3 epitope during the transition. -- Abstract: The characteristics of pluripotent embryonic stem cells of human and mouse are different. The properties of human embryonic stem cells (hESCs) are similar to those of mouse epiblast stem cells (mEpiSCs), which are in a later developmental pluripotency state, the so-called “primed state” compared to mouse embryonic stem cells (mESCs) which are in a naïve state. As a result of the properties of the primed state, hESCs proliferate slowly, cannot survive as single cells, and can only be transfected with genes at low efficiency. Generating hESCs in the naïve state is necessary to overcome these problems and allow their application in regenerative medicine. Therefore, clarifying the mechanism of the transition between the naïve and primed states in pluripotent stem cells is important for the establishment of stable methods of generating naïve state hESCs. However, the signaling pathways which contribute to the transition between the naïve and primed states are still unclear. In this study, we carried out induction from mESCs to mEpiSC-like cells (mEpiSCLCs), and observed an increase in the activation of Fas signaling during the induction. The expression of Fgf5, an epiblast marker, was diminished by inhibition of Fas signaling using the caspase-8 and -3 blocking peptides, IETD and DEVD, respectively. Furthermore, during the induction, we observed increased expression of 3-O sulfated heparan sulfate (HS) structures synthesized by HS 3-O-sulfotransferase (3OST), which are recognized by the HS4C3 antibody (HS4C3-binding epitope

Perbandingan Gabapentin 600 mg dengan 1.200 mg per Oral Preoperatif terhadap Nilai Visual Analogue Scale dan Pengurangan Kebutuhan Petidin Pascaoperasi pada Modifikasi Mastektomi Radikal

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Ardi Zulfariansyah

2013-12-01

Full Text Available Gabapentin is a GABA analog which has the effect of anti hyperalgesia, anti allodynia, and anti nociceptive. This research was conducted in order to assess the effect of 600mg and 1,200 mg gabapentin given preoperatively to assess visual analogue scale (VAS score and reduction of pethidine requirement. The study was done by conducting a double blind randomized controlled trial on 38 patients, aged 18–65 years, with ASA physical status I–II. Patients were divided into two groups: 600 mg gabapentin and 1,200 mg gabapentin group. The quality of pain was assessed using VAS score. The results were statistically analyzed using Mann-Whitney Test with 95% confidence interval and considered significant if p value <0.05. From the results, the VAS values obtained at rest and during mobilization were significantly different (p<0.05. The 1,200 mg gabapentin group received less additional pethidine (10.5% vs 15.8%, although no significant difference was shown (p=0.631. The conclusion of this study is that administration of 1,200 mg gabapentin per oral pre operatively is better when compared to 600 mg in reducing post operative visual analog scale score in modified radical mastectomy. However, it do not reduce the need for analgesic significantly.

Final Report DM1200 Tests With AZ 101 HLW Simulants VSL-03R3800-4, Rev. 0, 2/17/04

International Nuclear Information System (INIS)

Kruger, A.A.; Matlack, K.S.; Bardakci, T.; D'Angelo, N.A.; Gong, W.; Kot, W.K.; Pegg, I.L.

2011-01-01

This report documents melter and off-gas performance results obtained on the DM 1200 HLW Pilot Melter during processing of simulated HLW AZ-101 feed. The principal objectives of the DM1200 melter testing were to determine the achievable glass production rates for simulated HLW AZ-101 feed; determine the effect of bubbling rate and feed solids content on production rate; characterize melter off-gas emissions; characterize the performance of the prototypical off-gas system components as well as their integrated performance; characterize the feed, glass product, and off-gas effluents; and to perform pre- and post-test inspections of system components. The test objectives (including test success criteria), along with how they were met, are outlined in a table.

FINAL REPORT DM1200 TESTS WITH AZ 101 HLW SIMULANTS VSL-03R3800-4 REV 0 2/17/04

Energy Technology Data Exchange (ETDEWEB)

KRUGER AA; MATLACK KS; BARDAKCI T; D' ANGELO NA; GONG W; KOT WK; PEGG IL

2011-12-29

This report documents melter and off-gas performance results obtained on the DM 1200 HLW Pilot Melter during processing of simulated HLW AZ-101 feed. The principal objectives of the DM1200 melter testing were to determine the achievable glass production rates for simulated HLW AZ-101 feed; determine the effect of bubbling rate and feed solids content on production rate; characterize melter off-gas emissions; characterize the performance of the prototypical off-gas system components as well as their integrated performance; characterize the feed, glass product, and off-gas effluents; and to perform pre- and post-test inspections of system components. The test objectives (including test success criteria), along with how they were met, are outlined in a table.

Determination of different recreational drugs in sweat by headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME GC/MS): Application to drugged drivers.

Science.gov (United States)

Gentili, Stefano; Mortali, Claudia; Mastrobattista, Luisa; Berretta, Paolo; Zaami, Simona

2016-09-10

A procedure based on headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography/mass spectrometry (GC/MS) has been developed for the determination of most commonly used drugs of abuse in sweat of drivers stopped during roadside controls. DrugWipe 5A sweat screening device was used to collect sweat by a specific pad rubbed gently over forehead skin surface. The procedure involved an acid hydrolysis, a HS-SPME extraction for drugs of abuse but Δ(9)-tetrahydrocannabinol, which was directly extracted in alkaline medium HS-SPME conditions, a GC separation of analytes by a capillary column and MS detection by electron impact ionisation. The method was linear from the limit of quantification (LOQ) to 50ng drug per pad (r(2)≥0.99), with an intra- and inter-assay precision and accuracy always less than 15% and an analytical recovery between 95.1% and 102.8%, depending on the considered analyte. Using the validated method, sweat from 60 apparently intoxicated drivers were found positive to one or more drugs of abuse, showing sweat patches testing as a viable economic and simple alternative to conventional (blood and/or urine) and non conventional (oral fluid) testing of drugs of abuse in drugged drivers. Copyright © 2016 Elsevier B.V. All rights reserved.

Analysis of carbonyl compounds via headspace solid-phase microextraction with on-fiber derivatization and gas chromatographic-ion trap tandem mass spectrometric determination of their O-(2,3,4,5,6-pentafluorobenzyl)oxime derivatives

Energy Technology Data Exchange (ETDEWEB)

Schmarr, Hans-Georg [Dienstleistungszentrum Laendlicher Raum (DLR) Rheinpfalz, Breitenweg 71, D-67435 Neustadt an der Weinstrasse (Germany)], E-mail: hans-georg.schmarr@dlr.rlp.de; Potouridis, Theodoros; Ganss, Sebastian; Sang, Wei; Koepp, Benedikt; Bokuz, Ursula; Fischer, Ulrich [Dienstleistungszentrum Laendlicher Raum (DLR) Rheinpfalz, Breitenweg 71, D-67435 Neustadt an der Weinstrasse (Germany)

2008-06-09

An improved method for the analysis of carbonyls is described utilizing a headspace solid-phase microextraction (HS-SPME) step and on-fiber derivatization with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBHA) hydrochloride. Thermal desorption of the oxime derivatives formed on the fiber is followed by gas chromatographic separation coupled to an ion trap tandem mass spectrometer (GC-ITMS). Selecting specific fragment ions within the electron ionization (EI{sup +}) mass spectra of these oxime derivatives as precursor ions for MS-MS fragmentation provides a suitable method for the target analysis of individual carbonyl classes, such as alkanals, (E)-2-alkenals, (E,E)-2,4-alkadienals, and others. Retention indices on polar as well as on apolar stationary phases along with EI{sup +} mass spectra patterns are presented for a large set of oxime derivatives, giving valuable information needed for unambiguous assignment of substances in complex sample matrices. The fast sample preparation and derivatization step via HS-SPME can be automated and is applicable to a variety of biological samples and foodstuffs, allowing rapid and sensitive screening analyses of important aldehydic biomarkers and aroma active compounds.

On passive scalar derivative statistics in grid turbulence

International Nuclear Information System (INIS)

Tong, C.; Warhaft, Z.

1994-01-01

The probability density function, and related statistics, of scalar (temperature) derivative fluctuations in decaying grid turbulence with an imposed cross-stream, passive linear temperature profile, is studied for a turbulence Reynolds number range, Re l , varying from 50 to 1200, (corresponding to a Taylor Reynolds number range 30 λ θy has a value of 1.8±0.2 (twice the value observed in shear flows), and has no significant variation with Reynolds number. The ratio of the temperature derivative standard deviation along the gradient to that normal to it is approximately 1.2±0.1 also, with no variation with Re. The kurtosis of the derivatives increases approximately as Re 0.2 l . The results show that the rare, intense temperature deviations that produce the skewed scalar derivative, increase in frequency, but their area fraction (of the total field) becomes smaller as the Reynolds number increases. Thus, since S θy remains constant, they become sharper and more intense, occurring deeper in the tails of the probability density function. Measurements in a thermal mixing layer, which has a nonlinear mean temperature profile, are also presented, and these show a similar value of S θy to the linear profile case. The experiments broadly confirm the two-dimensional numerical simulations of Holzer and Siggia [Phys. Fluids (in press)], as well as other recent simulations, although there are some differences

Evaluation of Lumipulse® G1200 for the measurement of six tumor markers: Comparison with AIA® 2000.

Science.gov (United States)

de Rancher, Marie-Aude Robert; Oudart, Jean-Baptiste; Maquart, François-Xavier; Monboisse, Jean Claude; Ramont, Laurent

2016-11-01

Tumor marker assays are daily practiced, for screening and follow up of cancers. Interassay precision is an important parameter for the interpretation of the kinetics of the markers, in order to conclude to the efficiency or failure of treatment. The aim of this study was to compare two automated Immunoassay analyzers, Lumipulse® G1200 and AIA® 2000. Both analyzers used an immunoassay system but with different antibodies. Six tumor markers commonly used were studied: AFP, PSA, CA 19-9, CA 15-3, CA 125 and CEA. 253 samples have been collected over a period of one month and analyzed by both analyzers. Regression of Passing-Badblock and Bland-Altman diagram were used to analyze the results for AFP (n=36), PSA (n=39), CA-125 (n=40), CA 15-3 (n=40), CA 19-9 (n=46) and CEA (n=52) were performed. Analytical performances of Lumipulse® G1200 highlighted the good inter-run and intra-run precision of the analyzer. We obtained a good correlation coefficient between Lumipulse G1200® and AIA 2000®, >0.96 for most markers except CA 19-9 which provided a correlation coefficient significantly lower than that obtained with other markers. The concordance for all markers was >94% except for CA 19-9 (83.7%). This study showed a good correlation between the two analyzers and, therefore, a transfer from one analyzer to the other is possible for the different markers studied. However, we found here the classical difficulty to transfer this type of analysis, due to the absence of method standardization. This difficulty was particularly illustrated by CA19-9. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

The influence of amperage of electric arc on microhardness in the area single and overlapping remeltings of HS 6-5-2 steel

Directory of Open Access Journals (Sweden)

A. Dziedzic

2011-07-01

Full Text Available The present thesis depicts the microhardness of HV0,065 surface layer of high speed steel HS6-5-2 remelted with the electric arc. There were different surface layer variants of remelting used – the amperage was changed from 50 to 120A with the stable scanning speed of 300mm/min. There was also the influence of overlapping of the remeltings on the microhardness result. The highest average microhardness of the surface layer of high speed steel HS6-5-2 amounting 1100 HV0,065 was achieved by using the amperage of electric arc of 50 A. The overlapping of remeltings is connected with the possibility of occurence of the microhardness decrease in the area of overlapping of the heat influence zone of second remelting (another remelting on the first remelting (the previous one.

Development of a 1200 fine group nuclear data library for advanced nuclear systems

Institute of Scientific and Technical Information of China (English)

Jun Zou; Lei-Ming Shang; Fang Wang; Li-Juan Hao

2017-01-01

Accurate and reliable nuclear data libraries are essential for calculation and design of advanced nuclear systems.A 1200 fine group nuclear data library Hybrid Evaluated Nuclear Data Library/Fine Group (HENDL/FG) with neutrons of up to 150 MeV has been developed to improve the accuracy of neutronics calculations and analysis.Corrections of Doppler,resonance self-shielding,and thermal upscatter effects were done for HENDL/FG.Shielding and critical safety benchmarks were performed to test the accuracy and reliability of the library.The discrepancy between calculated and measured nuclear parameters fell into a reasonable range.

Liquid chromatography/tandem mass spectrometry method for quantitative estimation of solutol HS15 and its applications

OpenAIRE

Bhaskar, V. Vijaya; Middha, Anil; Srivastava, Pratima; Rajagopal, Sriram

2015-01-01

A rapid, sensitive and selective pseudoMRM (pMRM)-based method for the determination of solutol HS15 (SHS15) in rat plasma was developed using liquid chromatography/tandem mass spectrometry (LCâMS/MS). The most abundant ions corresponding to SHS15 free polyethyleneglycol (PEG) oligomers at m/z 481, 525, 569, 613, 657, 701, 745, 789, 833, 877, 921 and 965 were selected for pMRM in electrospray mode of ionization. Purity of the lipophilic and hydrophilic components of SHS15 was estimated using ...

Screening for Elder Abuse among Turkish Older People: Validity of the Hwalek-Sengstock Elder Abuse Screening Test (H-S "East")

Science.gov (United States)

Özmete, Emine; Megahead, Hamido A.

2017-01-01

Objective: This study aims to adapt "The Hwalek-Sengstock Elder Abuse Screening Test (H-S "East")" (Neale, Hwalek, Scott, Sengstock, & Stahl, 1991) to Turkish and to assess its validity and reliability while determining the factors that affect elder abuse. Method: The sample of the study is composed of 465 elderly women and…

Sistem Informasi Penjualan Pemasangan Kacafilm “HS Auto Film"

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Noer Tjahja Moekthi Pratjitana

2013-07-01

Full Text Available The growing market share in many business areas especially in service area is causing the use of conventional accounting system being ineffective. One of the companies which still using the conventional one is a window film set-up service company called HS Auto Film. The process of processing sales data is less accurate and slower in the contrary of the developing sales data.  Computer-based window film set-up Sales Information System is built in order to make the processing data more accurate and faster.  The information system is built using Visual Basic programming language along with .NET framework technology and SQL Server database that embedded in the application itself. The making and development process of the Sales Information System is using waterfall method.  Waterfall method consists of user requirement, analysis, design, implementation, and testing. Entity Relationship Diagram, Data Flow Diagram and Flowchart are also used in the Information System design. The output of this application design is a desktop application that can help user to control the set-up of window film, manage payment bill, and manage document in accounting in faster and more accurate way. The information system can be developed later using web-based system so that the development and maintenance can be done easier.

The 5HT(1A) receptor ligand, S15535, antagonises G-protein activation: a [35S]GTPgammaS and [3H]S15535 autoradiography study.

Science.gov (United States)

Newman-Tancredi, A; Rivet, J; Chaput, C; Touzard, M; Verrièle, L; Millan, M J

1999-11-19

4-(Benzodioxan-5-yl)1-(indan-2-yl)piperazine (S15535) is a highly selective ligand at 5-HT(1A) receptors. The present study compared its autoradiographic labelling of rat brain sections with its functional actions, visualised by guanylyl-5'-[gamma-thio]-triphosphate ([35S]GTPgammaS) autoradiography, which affords a measure of G-protein activation. [3H]S15535 binding was highest in hippocampus, frontal cortex, entorhinal cortex, lateral septum, interpeduncular nucleus and dorsal raphe, consistent with specific labelling of 5-HT(1A) receptors. In functional studies, S15535 (10 microM) did not markedly stimulate G-protein activation in any brain region, but abolished the activation induced by the selective 5-HT(1A) agonist, (+)-8-hydroxy-dipropyl-aminotetralin ((+)-8-OH-DPAT, 1 microM), in structures enriched in [3H]S15535 labelling. S15535 did not block 5-HT-stimulated activation in caudate nucleus or substantia nigra, regions where (+)-8-OH-DPAT was ineffective and [3H]S15535 binding was absent. Interestingly, S15535 attenuated (+)-8-OH-DPAT and 5-HT-stimulated G-protein activation in dorsal raphe, a region in which S15535 is known to exhibit agonist properties in vivo [Lejeune, F., Millan, M.J., 1998. Induction of burst firing in ventral tegmental area dopaminergic neurons by activation of serotonin (5-HT)(1A) receptors: WAY100,635-reversible actions of the highly selective ligands, flesinoxan and S15535. Synapse 30, 172-180.]. The present data show that (i) [3H]S15535 labels pre- and post-synaptic populations of 5-HT(1A) sites in rat brain sections, (ii) S15535 exhibits antagonist properties at post-synaptic 5-HT(1A) receptors in corticolimbic regions, and (iii) S15535 also attenuates agonist-stimulated G-protein activation at raphe-localised 5-HT(1A) receptors.

Neutron skyshine measurement at a K1200 superconducting heavy ion cyclotron using bubble dosimeters

Energy Technology Data Exchange (ETDEWEB)

Mukherjee, B. [Safety Div., Australian Nuclear Science and Technology Organisation, Menai (Australia); Ronningen, R.M. [Michigan State Univ., National Superconducting Cyclotron Lab., East Lansing, MI (United States); Rossi, P. [Michigan State Univ., Office of Radiation, Chemical and Biological Safety, East Lansing, MI (United States)

1999-07-01

Understanding the characteristics of the neutron skyshine radiation is necessary for an accurate assessment of the environmental dose in the vicinity of the containment of a high-energy particle accelerator. At the National Superconducting Cyclotron Laboratory (NSCL), neutron skyshine was measured, using beams of 140 MeV/nucleon {sup 4}He and 80 MeV/nucleon {sup 22}Ne ions from the K1200 superconducting cyclotron. After passing through a radioactive-beam production target, the ion beam stopped in a solid aluminium stopping bar inside of a dipole magnet, resulting in the production of high energy fragmentation as well as evaporation neutrons in the NSCL Analysis Hall. The neutron dose equivalent and energy spectrum at the 1.37 m thick concrete roof of the Analysis Hall, directly above the aluminium target bar (reference point), were estimated, using a spherical 'rem-counter' and a set of seven Bonner-spheres, respectively. The skyshine dose, from neutrons transmitted through 21.5-cm local iron 'shielding' of the dipole magnet and the concrete roof, were evaluated using superheated bubble dosimeters at 50 m, 75 m, 100 m and 115 m from the reference point. The neutron doses beyond the extremity of the NSCL facility were extrapolated from the results of this investigation and were used to predict the exposure to members of the public by considering the operation schedule of the K1200 cyclotron. (authors)

A brain-derived neurotrophic factor polymorphism Val66Met identifies fibromyalgia syndrome subgroup with higher body mass index and C-reactive protein.

Science.gov (United States)

Xiao, Yangming; Russell, I Jon; Liu, Ya-Guang

2012-08-01

A common single nucleotide polymorphism (SNP) in the gene of brain-derived neurotrophic factor (BDNF) results from a substitution at position 66 from valine (Val) to methionine (Met) and may predispose to human neuropsychiatric disorders. We proposed to determine whether these BDNF gene SNPs were associated with fibromyalgia syndrome (FMS) and/or any of its typical phenotypes. Patients with FMS (N = 95) and healthy normal controls (HNC, N = 58) were studied. Serum high-sensitivity C-reactive protein (hsCRP) levels were measured using an enzyme-linked immunosorbent assay (ELISA). The BDNF SNPs were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The BDNF SNP distribution was 65 (68%) Val/Val, 28 (30%) Val/Met, and 2 (2%) Met/Met for FMS and 40 (69%), 17(29%), and 1 (2%) for HNC, respectively. The serum high-sensitivity C-reactive protein (hsCRP)and body mass index (BMI) in FMS were higher than in HNC. The FMS with BDNF Val66Val had significantly higher mean BMI (P = 0.0001) and hsCRP (P = 0.02) than did FMS carrying the Val66Met genotype. This pattern was not found in HNC. Phenotypic measures of subjective pain, pain threshold, depression, or insomnia did not relate to either of the BDNF SNPs in FMS. The relative distribution BDNF SNPs did not differ between FMS and HNC. The BDNF Val66Met polymorphism is not selective for FMS. The BDNF Val66Val SNP identifies a subgroup of FMS with elevated hsCRP and higher BMI. This is the first study to associate a BDNF polymorphism with a FMS subgroup phenotype.

Getting to Know a Place: Built Environment Walkability and Children's Spatial Representation of Their Home-School (h-s) Route.

Science.gov (United States)

Moran, Mika R; Eizenberg, Efrat; Plaut, Pnina

2017-06-06

The literature on environmental walkability to date has mainly focused on walking and related health outcomes. While previous studies suggest associations between walking and spatial knowledge, the associations between environmental walkability and spatial knowledge is yet to be explored. The current study addresses this lacuna in research by exploring children's mental representations of their home-school (h-s) route, vis.

DAYA SAING PRODUK TURUNAN SUSU INDONESIA DI PASAR DUNIA

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Lina Yanti

2012-06-01

Full Text Available ABSTRACTThis research purposes were (1 to measures the competitiveness of Indonesian milk derivative product in international market based on its comparative advantage, (2 to analyze the dynamics of Indonesian milk and also to analyze the commodity’s competitive position in the international market through its dynamic export product performance, (3 to analyze dominant factors that influencing Indonesian dairy export growth based on constant market share analysis. The analysis methods used to measure the competitiveness of Indonesian milk in this research are based on revealed comparative advantage analysis, Export product dynamic analysis (EPD, and constant market share analysis. The result from RCA showed that the Indonesian milk derivative products which are Milk not concentrated nor sweetened 1–6% fat (HS 040120, Milk and cream, concentrated or sweetened (HS 0402 and Buttermilk, curdled milk, cream, kephir, etc (HS 040390 do not have comparative advantage but for Milk and cream powder unsweetened > 1,5%  fat (HS 040221, Milk and cream nes sweetened or concentrated (HS 040299 and Cereal, flour, starch, milk preparations and products (HS19 have comparative advantage. The EPD found that all Indonesian milk derivative products are included in the Rising Star position. The result test using CMS analysis concluded that the most significant factor which affect the export growth for Indonesian HS 040390 and HS 0402 derivative products are the import growth effect, HS 19 is mainly dominated by commodity composition effect, and the export growth for the rest derivative products are dominated with the competitiveness effect. Therefore, it needs to be implemented efforts to enhance the competitiveness of dairy derived products IndonesiaKeywords: milk, competitiveness, revealed comparative advantage (RCA, export product dynamic (EPD, constant market share analysis (CMSAABSTRAKPenelitian ini bertujuan (1 menganalisis daya saing komoditas susu

Predictive value of CHA2DS2-VASc and CHA2DS2-VASc-HS scores for failed reperfusion after thrombolytic therapy in patients with ST-elevation myocardial ınfarction.

Science.gov (United States)

Kilic, Salih; Kocabas, Umut; Can, Levent Hurkan; Yavuzgil, Oğuz; Çetin, Mustafa; Zoghi, Mehdi

2018-03-07

Thrombolytic therapy is recommended for patients with acute ST-segment elevation myocardial infarction (STEMI) who cannot undergo primary percutaneous coronary intervention within the first 120 min. The aim of this study wasz to demonstrate the value of CHA₂DS₂-VASc and CHA₂DS₂-VASc-HS scores in predicting failed reperfusion in STEMI patients treated with thrombolytic therapy. A total of 537 consecutive patients were enrolled in the study; 139 had failed thrombolysis while the remaining 398 fulfilled the criteria for successful thrombolysis. Thrombolysis failure was defined with the lack of symptom relief, < 50% ST resolution-related electrocardiography within 90 min from initiation of the thrombolytic therapy, presence of hemodynamic or electrical instability or in-hospital mortality. CHA₂DS₂-VASc and CHA₂DS₂-VASc-HS scores, which incorporate hyperlipidemia, smoking, switches between female and male gender, were previously shown to be markers of the severity of coronary artery disease (CAD). History of hypertension, diabetes mellitus, hyperlipidemia, heart failure, smoking, and CAD were significantly common in failed reperfusion patients (for all; p < 0.05). For prediction of failed reperfusion, the cut-off value of CHA₂DS₂-VASc score was ≥ 2 with a sensitivity of 80.90% and a specificity of 41.01% (area under curve [AUC] 0.660; 95% confidence interval [CI] 0.618-0.700; p < 0.001) and the cut-off value of CHA₂DS₂-VASc-HS score was ≥ 3 with a sensitivity of 76.13% and a specificity of 67.63% (AUC 0.764; 95% CI 0.725-0.799; p < 0.001). The CHA₂DS₂-VASc-HS score was found to be statistically and significantly better than CHA₂DS₂-VASc score to predict failed reperfusion (p < 0.001). The findings suggest that the CHA₂DS₂-VASc and especially CHA₂DS₂-VASc-HS scores could be considered as predictors of risk of failed reperfusion in STEMI patients.

Transcription Profiling Demonstrates Epigenetic Control of Non-retroviral RNA Virus-Derived Elements in the Human Genome

Directory of Open Access Journals (Sweden)

Kozue Sofuku

2015-09-01

Full Text Available Endogenous bornavirus-like nucleoprotein elements (EBLNs are DNA sequences in vertebrate genomes formed by the retrotransposon-mediated integration of ancient bornavirus sequence. Thus, EBLNs evidence a mechanism of retrotransposon-mediated RNA-to-DNA information flow from environment to animals. Although EBLNs are non-transposable, they share some features with retrotransposons. Here, to test whether hosts control the expression of EBLNs similarly to retrotransposons, we profiled the transcription of all Homo sapiens EBLNs (hsEBLN-1 to hsEBLN-7. We could detect transcription of all hsEBLNs in at least one tissue. Among them, hsEBLN-1 is transcribed almost exclusively in the testis. In most tissues, expression from the hsEBLN-1 locus is silenced epigenetically. Finally, we showed the possibility that hsEBLN-1 integration at this locus affects the expression of a neighboring gene. Our results suggest that hosts regulate the expression of endogenous non-retroviral virus elements similarly to how they regulate the expression of retrotransposons, possibly contributing to new transcripts and regulatory complexity to the human genome.

Comparison of LUMIPULSE(®) G1200 With Kryptor and Modular E170 for the Measurement of Seven Tumor Markers.

Science.gov (United States)

Marlet, Julien; Bernard, Maguy

2016-01-01

Tumor marker measurements are becoming essential for prognosis and follow-up of patients in oncology. In this context, we aimed to compare a new analyzer, Lumipulse(®) G1200 (Fujirebio group, distributed in Europe by the Innogenetics group) with Kryptor(®) (Thermo Fisher Scientific B.R.A.H.M.S, Asnières, France) and Modular(®) Elecsys E170 (Roche Diagnostics, Meylan, France) for the measurement of seven tumor markers: PSA, AFP, CEA, CA 15-3, CA 125, CA 19-9, and Cyfra 21-1. A total of 471 serum samples from patients with elevated tumor markers and 100 serum from healthy patients were analyzed with Lumipulse(®) G1200 and either Kryptor(®) (for AFP) or Modular(®) (for the six other markers). The good precision of Lumipulse(®) G1200 assays was confirmed with CVs Lumipulse results were well correlated with Modular or Kryptor results (r ≥ 0.94). Concordance of results interpretation was > 95% and tumor marker kinetics were all similar. We confirmed the analytical performances of Lumipulse(®) tumor marker assays except for the CYFRA 21-1 assay for which performances were poor in this study. We noticed a few discrepancies for the CEA assay. Besides, values obtained for CA 19-9 were higher with Lumipulse leading to a bias (slope = 1.5). But for the four other tumor markers assays (PSA, AFP, CA 125, CA 15-3), the results were directly transferable between Lumipulse and Kryptor or Modular, thus facilitating an eventual substitution of one system by another. © 2014 Wiley Periodicals, Inc.

[Establishment of Reference Value of Hs-cTnT in Sichuan Region and Its Diagnostic Value in Patients with Chest Pain].

Science.gov (United States)

Guo, Ying; Du, Xiang-Yang; Huang, Hua-Lan; Wang, Wei-Qing; Nie, Xin; Li, Gui-Xing

2017-11-01

To establish the reference value of high sensitive cardiac troponin T (hs-cTnT) and the efficiency of reference value in the diagnosis of chest pain. Volunteers from eight independent communities in Chengdu,Sichuan were selected with detailed records of physical examination,electrocardiogram,ultrasound examination. The level of hs-cTnT for healthy volunteers was tested to determine ninety-ninth percentile references according to sex and ages. 2 249 patients with chest pain in the emergency department of Western China Hospital from July 2009 to July 2014 were enrolled to measure the efficiency of reference value for diagnosing acute myocardial infarction (AMI). There were 1 305 volunteers included finally. Among them,the mean hs-cTnT level of male was 4.3 (3.2-5.9) ng/L,which was significantly higher than that of female 3.0 (3.0-3.1) ng/L ( P value was less than 0.01. The 99th percentile values of male were 10.8 ng/L,15.4 ng/L and 19.7 ng/L for values of female were 4.6 ng/L,8.9 ng/L,18.8 ng/L,respectively. There was no difference in sensitivity and specificity between the value we figured out and manufactures provided (14.0 ng/L) for thosevalue did not show diversity ( P >0.05) but the specificity and positive predictive value showed significant difference (male: 0.67 vs. 0.56 and 0.83 vs. 0.79, P value of patients<60 yr. should be 14.0 ng/L,while 20.0 ng/L for those patients≥60 yr.

Characterization of the volatile components in green tea by IRAE-HS-SPME/GC-MS combined with multivariate analysis.

Science.gov (United States)

Yang, Yan-Qin; Yin, Hong-Xu; Yuan, Hai-Bo; Jiang, Yong-Wen; Dong, Chun-Wang; Deng, Yu-Liang

2018-01-01

In the present work, a novel infrared-assisted extraction coupled to headspace solid-phase microextraction (IRAE-HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) was developed for rapid determination of the volatile components in green tea. The extraction parameters such as fiber type, sample amount, infrared power, extraction time, and infrared lamp distance were optimized by orthogonal experimental design. Under optimum conditions, a total of 82 volatile compounds in 21 green tea samples from different geographical origins were identified. Compared with classical water-bath heating, the proposed technique has remarkable advantages of considerably reducing the analytical time and high efficiency. In addition, an effective classification of green teas based on their volatile profiles was achieved by partial least square-discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). Furthermore, the application of a dual criterion based on the variable importance in the projection (VIP) values of the PLS-DA models and on the category from one-way univariate analysis (ANOVA) allowed the identification of 12 potential volatile markers, which were considered to make the most important contribution to the discrimination of the samples. The results suggest that IRAE-HS-SPME/GC-MS technique combined with multivariate analysis offers a valuable tool to assess geographical traceability of different tea varieties.

Characterization of the volatile components in green tea by IRAE-HS-SPME/GC-MS combined with multivariate analysis.

Directory of Open Access Journals (Sweden)

Yan-Qin Yang

Full Text Available In the present work, a novel infrared-assisted extraction coupled to headspace solid-phase microextraction (IRAE-HS-SPME followed by gas chromatography-mass spectrometry (GC-MS was developed for rapid determination of the volatile components in green tea. The extraction parameters such as fiber type, sample amount, infrared power, extraction time, and infrared lamp distance were optimized by orthogonal experimental design. Under optimum conditions, a total of 82 volatile compounds in 21 green tea samples from different geographical origins were identified. Compared with classical water-bath heating, the proposed technique has remarkable advantages of considerably reducing the analytical time and high efficiency. In addition, an effective classification of green teas based on their volatile profiles was achieved by partial least square-discriminant analysis (PLS-DA and hierarchical clustering analysis (HCA. Furthermore, the application of a dual criterion based on the variable importance in the projection (VIP values of the PLS-DA models and on the category from one-way univariate analysis (ANOVA allowed the identification of 12 potential volatile markers, which were considered to make the most important contribution to the discrimination of the samples. The results suggest that IRAE-HS-SPME/GC-MS technique combined with multivariate analysis offers a valuable tool to assess geographical traceability of different tea varieties.

hs-CRP is strongly associated with coronary heart disease (CHD): A data mining approach using decision tree algorithm.

Science.gov (United States)

Tayefi, Maryam; Tajfard, Mohammad; Saffar, Sara; Hanachi, Parichehr; Amirabadizadeh, Ali Reza; Esmaeily, Habibollah; Taghipour, Ali; Ferns, Gordon A; Moohebati, Mohsen; Ghayour-Mobarhan, Majid

2017-04-01

Coronary heart disease (CHD) is an important public health problem globally. Algorithms incorporating the assessment of clinical biomarkers together with several established traditional risk factors can help clinicians to predict CHD and support clinical decision making with respect to interventions. Decision tree (DT) is a data mining model for extracting hidden knowledge from large databases. We aimed to establish a predictive model for coronary heart disease using a decision tree algorithm. Here we used a dataset of 2346 individuals including 1159 healthy participants and 1187 participant who had undergone coronary angiography (405 participants with negative angiography and 782 participants with positive angiography). We entered 10 variables of a total 12 variables into the DT algorithm (including age, sex, FBG, TG, hs-CRP, TC, HDL, LDL, SBP and DBP). Our model could identify the associated risk factors of CHD with sensitivity, specificity, accuracy of 96%, 87%, 94% and respectively. Serum hs-CRP levels was at top of the tree in our model, following by FBG, gender and age. Our model appears to be an accurate, specific and sensitive model for identifying the presence of CHD, but will require validation in prospective studies. Copyright © 2017 Elsevier B.V. All rights reserved.

Comparison of the atmospheric- and reduced-pressure HS-SPME strategies for analysis of residual solvents in commercial antibiotics using a steel fiber coated with a multiwalled carbon nanotube/polyaniline nanocomposite.

Science.gov (United States)

Ghiasvand, Ali Reza; Nouriasl, Kolsoum; Yazdankhah, Fatemeh

2018-01-01

A low-cost, sensitive and reliable reduced-pressure headspace solid-phase microextraction (HS-SPME) setup was developed and evaluated for direct extraction of residual solvents in commercial antibiotics, followed by determination by gas chromatography with flame ionization detection (GC-FID). A stainless steel narrow wire was made porous and adhesive by platinization by a modified electrophoretic deposition method and coated with a polyaniline/multiwalled carbon nanotube nanocomposite. All experimental variables affecting the extraction efficiency were investigated for both atmospheric-pressure and reduced-pressure conditions. Comparison of the optimal experimental conditions and the results demonstrated that the reduced-pressure strategy leads to a remarkable increase in the extraction efficiency and reduction of the extraction time and temperature (10 min, 25 °Ϲ vs 20 min, 40 °Ϲ). Additionally, the reduced-pressure strategy showed better analytical performances compared with those obtained by the conventional HS-SPME-GC-FID method. Limit of detections, linear dynamic ranges, and relative standard deviations of the reduced-pressure HS-SPME procedure for benzene, toluene, ethylbenzene, and xylene (BTEX) in injectable solid drugs were obtained over the ranges of 20-100 pg g -1 , 0.02-40 μg g -1 , and 2.8-10.2%, respectively. The procedure developed was successful for the analysis of BTEX in commercial containers of penicillin, ampicillin, ceftriaxone, and cefazolin. Graphical abstract Schematic representation of the developed RP-HS-SPME setup.

A high area, porous and resistant platinized stainless steel fiber coated by nanostructured polypyrrole for direct HS-SPME of nicotine in biological samples prior to GC-FID quantification.

Science.gov (United States)

Abdolhosseini, Sana; Ghiasvand, Alireza; Heidari, Nahid

2017-09-01

The surface of a stainless steel fiber was made porous, resistant and cohesive using electrophoretic deposition and coated by the nanostructured polypyrrole using an amended in-situ electropolymerization method. The coated fiber was applied for direct extraction of nicotine in biological samples through a headspace solid-phase microextraction (HS-SPME) method followed by GC-FID determination. The effects of the important experimental variables on the efficiency of the developed HS-SPME-GC-FID method, including pH of sample solution, extraction temperature and time, stirring rate, and ionic strength were evaluated and optimized. Under the optimal experimental conditions, the calibration curve was linear over the range of 0.1-20μgmL -1 and the detection limit was obtained 20ngmL -1 . Relative standard deviation (RSD, n=6) was calculated 7.6%. The results demonstrated the superiority of the proposed fiber compared with the most used commercial types. The proposed HS-SPME-GC-FID method was successfully used for the analysis of nicotine in urine and human plasma samples. Copyright © 2017 Elsevier B.V. All rights reserved.

Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Endoscopic and histological outcomes.

Science.gov (United States)

Pérez-Merino, E M; Usón-Casaús, J M; Duque-Carrasco, J; Zaragoza-Bayle, C; Mariñas-Pardo, L; Hermida-Prieto, M; Vilafranca-Compte, M; Barrera-Chacón, R; Gualtieri, M

2015-12-01

Systemic administration of mesenchymal stem cells (MSCs) has been shown to be safe and efficacious in humans with Crohn's disease. The aim of this study was to evaluate the safety of an intravenous (IV) infusion of adipose tissue-derived mesenchymal stem cells (ASCs) and to assess macroscopic and histological effects in the digestive tract of dogs with inflammatory bowel disease (IBD). Eleven dogs with confirmed IBD received a single ASC infusion (2 × 10(6) cells/kg bodyweight). Full digestive endoscopic evaluation was performed pre-treatment and between 90 and 120 days post-treatment with mucosal changes being assessed using a fit-for-purpose endoscopic scale. Endoscopic biopsies from each digestive section were evaluated histologically according to the World Small Animal Veterinary Association (WSAVA) Gastrointestinal Standardization Group criteria. The pre- and post-treatment canine IBD endoscopic index (CIBDEI) and histological score (HS) were calculated and compared using the Wilcoxon test. Remission was defined as a reduction of >75% of the CIBDEI and HS compared with pre-treatment. No acute reactions to ASC infusion or side effects were reported in any dog. Significant differences between pre- and post-treatment were found in both the CIBDEI (P = 0.004) and HS (P = 0.004). Endoscopic remission occurred in 4/11 dogs with the remaining dogs showing decreased CIBDEI (44.8% to 73.3%). Histological remission was not achieved in any dog, with an average reduction of the pre-treatment HS of 27.2%. In conclusion, a single IV infusion of allogeneic ASCs improved gastrointestinal lesions as assessed macroscopically and slightly reduced gastrointestinal inflammation as evaluated by histopathology in dogs with IBD. Copyright © 2015 Elsevier Ltd. All rights reserved.

WTG Energy Systems' MP1-200 200 kilowatt wind turbine generator. [a fixed pitch rotor configuration driving a synchronous generator

Science.gov (United States)

Spaulding, A. P., Jr.

1979-01-01

The preliminary design criteria of the MP1-200 wind turbine are given along with a brief description of the wind turbine generator. Performance and operational experience and cost factors are included. Recommendations for additional research are listed.

Fine-structural effects of 1200-R abdominal x irradiation on rat intestinal epithelium

International Nuclear Information System (INIS)

Lieb, R.J.; McDonald, T.F.; McKenney, J.R.

1977-01-01

Male Charles River CD rats were shielded from the xiphoid process cranially with lead and were exposed to 1200-R abdominal x irradiation. Animals were sacrificed at 1 through 4 days following irradiation and tissues from both ileum and jejunum were prepared for electron microscopic examination. At the fine-structural level early changes were confined to a proliferation and dilation of smooth endoplasmic reticulum and to an increase in the number of lysosomes. At 4 days postirradiation, cells covering the villi were cuboidal rather than columnar and appeared to be immature crypt-type cells. The appearance of these cells was coincident with the onset of diarrhea in these animals

Enhancement of Solubility, Dissolution rate and Bioavailability of Efavirenz by Cyclodextrins and Solutol HS15 - A Factorial Study

OpenAIRE

R. Yogananda; K. P. R. Chowdary

2013-01-01

Efavirenz widely prescribed anti-retroviral drug belongs to class II BCS and exhibit low and variable oral bioavailability due to its poor aqueous solubility and it requires enhancement in solubility and dissolution rate for increasing its oral bioavailability. The objective of the present investigation is to enhance the solubility, dissolution rate and bioavailability of efavirenz by the use of cyclodextrins (%CD and HP%CD) and surfactant, Solutol HS15. The individual main effects and combin...

INTEGRATED DM 1200 MELTER TESTING OF HLW C-106/AY-102 COMPOSITION USING BUBBLERS VSL-03R3800-1 REV 0 9/15/03

Energy Technology Data Exchange (ETDEWEB)

KRUGER AA; MATLACK KS; GONG W; BARDAKCI T; D' ANGELO NA; KOT WK; PEGG IL

2011-12-29

This report documents melter and off-gas performance results obtained on the DM1200 HLW Pilot Melter during processing of simulated HLW C-106/AY-102 feed. The principal objectives of the DM1200 melter testing were to determine the achievable glass production rates for simulated HLW C-106/AY-102 feed; determine the effect of bubbling rate on production rate; characterize melter off-gas emissions; characterize the performance of the prototypical off-gas system components as well as their integrated performance; characterize the feed, glass product, and off-gas effluents; and to perform pre- and post test inspections of system components.

Integrated DM 1200 Melter Testing Of HLW C-106/AY-102 Composition Using Bubblers VSL-03R3800-1, Rev. 0, 9/15/03

International Nuclear Information System (INIS)

Kruger, A.A.; Matlack, K.S.; Kot, W.K.; Bardakci, T.; Gong, W.; D'Angelo, N.A.; Pegg, I.L.

2011-01-01

This report documents melter and off-gas performance results obtained on the DM1200 HLW Pilot Melter during processing of simulated HLW C-106/AY-102 feed. The principal objectives of the DM1200 melter testing were to determine the achievable glass production rates for simulated HLW C-106/AY-102 feed; determine the effect of bubbling rate on production rate; characterize melter off-gas emissions; characterize the performance of the prototypical off-gas system components as well as their integrated performance; characterize the feed, glass product, and off-gas effluents; and to perform pre- and post test inspections of system components.

La huaca Narihualá: un documento para la etnohistoria de la Costa Norte del Perú (1000 - 1200 D.-C.

Directory of Open Access Journals (Sweden)

1990-01-01

Full Text Available LA HUACA NARIHUALA: UN DOCUMENT POUR L’ETHNOHISTOIRE DE LA REGION COTIERE DU PEROU (1000 - 1200 AP. J. C.. Le site archéologique de Narihualá, situé sur la côte de l’extrème nord du Pérou, fut un centre politique et administratif important de l’ethnie Tallán qui occupait la basse vallée de Piura pendant la période Intermédiaire Tardive (1000-1200 ap. J.C.. Dans cet article, l’auteur procède à l’analyse de façon exhaustive un document ethnohistorique daté de l656, décrivant les fouilles effectueés localement par des indigènes ainsi que celles réalisées officiellement par la Couronne d’Espagne au moyen de contrats. Le document est ensuite confronté avec les résultats des recherches archéologiques du mois de mai l988 qui avaient permis d’identifier des fragments de céramique de style Tallán, Chimú Tardif et Inca Local, et de localiser des chemins pré-hispaniques. El sitio arqueológico Narihualá, localizado en la costa del extremo norte del Perú, fue un importante centro políticoadministrativo del área étnica de los Tallán del Bajo Piura y corresponde al Período Intermedio Tardío (1000-1200 d. C.. En este artículo, el autor analiza exhaustivamente un documento etnohistórico que data de l656, describiendo las excavaciones de indígenas y las oficializadas por la Corona española mediante contratos aprobados. Se confronta tal documento con los reconocimientos arqueológicos realizados en el mes de mayo de l988, que permitieron identificar fragmentos de cerámica del estilo Tallán, Chimú Tardío e Inca Local y ubicar antiguos caminos prehispánicos. THE HUACA NARIHUALA: A DOCUMENT FOR THE ETHNOHISTORY OF THE NORTH COAST OF PERU (1000-1200 A.D.. Narihuala, is an arqueological site located on the Northern coast of Peru. During the Late Intermediate period (1000-1200 A.D. it was an important political and administrative center of the Tallán ethnic group, of the Lower Piura valley. In this paper, the author

Comparison of fuel cycles characteristics for nuclear energy systems based on WWER-TOI and BN-1200 reactors

International Nuclear Information System (INIS)

Kagramanyan, V.S.; Kalashnikov, A.G.; Kapranova, Eh.N.; Puzakov, A.Yu.

2014-01-01

Authors determine the characteristics of the fuel cycle (FC) based on stationary nuclear power system based on WWER-TOI and BN-1200 reactors with fuel of different composition. Characteristics of reactor systems with partial or complete spent nuclear fuel reprocessing and recycling of plutonium are compared to those of the reference system consisting only of WWER-TOI with uranium oxide fuel, operating in an open FC [ru

Retention data of bile acids and their oxo derivatives in characterization of pharmacokinetic properties and in silico ADME modeling.

Science.gov (United States)

Trifunović, Jovana; Borčić, Vladan; Vukmirović, Saša; Kon, Svetlana Goločorbin; Mikov, Momir

2016-09-20

Information on ADME properties of examined bile acids and their oxo derivatives are scarce, although the interest for bile acids and their use in nanochemistry and macromolecular chemistry is increasing. The purpose of this research was to evaluate the lipophilicity, a crucial physicochemical parameter for describing ADME properties of selected bile acids and their oxo derivatives, and to compare two approaches: experimentally determined hydrophobicity parameters and calculated logP values. Commercially available bile acids - deoxycholic, chenodeoxycholic, hyodeoxycholic and ursodeoxycholic acid were used to synthesize oxo derivatives. Lipophilicity was evaluated in two solvent systems: toluene/ethanol and toluene/butanol. Retention parameters were acquired by normal-phase TLC. The correlations between calculated logP values obtained using five different software and experimentally determined hydrophobicity parameters (RM(0)(tol/eth), RM(0)(tol/but), b(tol/eth) and b(tol/but)) were examined. Correlation analysis confirmed significant dependence between experimental RM(0) values and software calculated parameters. Results suggest satisfactory intestinal absorption after oral administration for all of the examined compounds as well as low volumes of distribution, and high affinity for binding with plasma proteins. Penetration through blood-brain barrier and skin is not satisfactory. All of the examined compounds show high affinity for binding with G-protein coupled receptors and consequently inhibition of ionic channels. Results also suggest possible binding with nuclear receptors. Established lipophilicity testing model of studied compounds showed excellent predictive ability and might represent significant tool in development of relations between chromatographic behavior and ADME properties. Compounds 3α-hydroxy-7,12-dioxo-5β-cholanoic and 12α-hydroxy-3,7-dioxo-5β-cholanoic acid might be the most suitable candidates for further development studies (satisfactory

1200 FPD refuelling simulation of RUFIC fuel in a CANDU 6 reactor

Energy Technology Data Exchange (ETDEWEB)

Kim, Soon Young; Jeong, Chang Joon; Min, Byung Joo; Suk, Ho Chun

2001-07-01

The refuelling strategy of RUFIC (Recovered Uranium Fuel in CANDU) fuel as a high-burnup fuel for a CANDU 6 reactor is studied to determine the achievable operation characteristics of the fuel and reactor. In this study, three refuelling schemes of 4-, 2-, and 3-bundle shift for 0.92 w/o RUFIC fuel in an CANDU 6 reactor were individually evaluated through 1200 FPD(Full Power Day)refuelling simulaltions where the 0.92 w/o RUFIC is equivalent to CANFLEX 0.9 w/o SEU(Slightly Enriched Uranium) in reactivity and burnup respects. The computer code system used for this study is WIMS-AECL/DRAGON/RFSP. The results simulated for the case of 4-bundle shift refueling scheme shows that the peak maximum channel power and peak maximum CPPF(Channel Power Peaking Factor)of 7228 kW and 1.175, respectively, seems too high to maintain the available operating margins, because some data of the maximum channel power exceed the operating limit(7070 kW based on the Technical Specifications of Wolsong 3 and 4 Units). Whereas, the results simulated for the case of 2-bundle shift refuelling scheme shows that sufficient operating margin could be secured where the peak maximum channel power and peak maximum CPPF were 6889 kW and 1.094, respectively. However, the channel refuelling rate (channels/day) of the 2-bundle shift refuelling scheme is twice that of the 4-bundle shift refuelling scheme, and hence the 2-bundle shift refuelling would not be an economical refuelling scheme for the RUFIC fuel bundles. Therefore, a 3-bundle shift refuelling scheme for the RUFIC fuel in CANDU 6 reactor was also studied by the 1200 FPD refuelling simulation. As a result, it is found that all the operating parameters in the 3-bundle shift case are achivable for the CANDU 6 reactor operation, and the channel refuelling rate of 2.88 channels/day seems to be attractive compared to the refuelling rate of 4.32 channels/day in the 2-bundle shift case.

Blood hsCRP And PGE2 Content With Clinical Outcome Using Modified Fenestrat Restorative Spinoplasty Better Than Lamonectomy-Fusion In Lumbar Stenosis

Directory of Open Access Journals (Sweden)

T Mahadewa

2012-05-01

Full Text Available Objective: Modified Fenestration-Restorative Spinoplasty (MFRS technique is an alternative to lumbar stenosis treatment, providing the equal decompression comparing with laminectomy techniques, without the implant, less expensive and complication rates. The purpose of this study was to determine which technique gives better inflammation and clinical outcome based on high sensitive C-Reactive Protein biomarker (hsCRP and Prostaglandin E2 (PGE 2, Visual Analog Scale (VAS of the day 7th postsurgery and ODI scores 3rd month post surgery. Methods: This study design is an experimental pretest-posttest randomized control group design. Results: This study results showed that the mean levels of hsCRP day 7th postsurgery were differ significantly between MFRS (23,09 ± 15,3 mg/L compared to LF (39,53 ± 24,4 mg/L. Likewise for the mean levels of PGE2 day 7th postsurgery were differ significantly between MFRS (491,39 ± 528,5 pg/ml compared to LF (1103,7 ± 1033,6 pg/ml at the significance level of p <0.05. MFRS clinical outcomes better than LF (p <0.05, for means of VAS value day 7th postsurgery and ODI score 3rd month postsurgery. Perioperative variable analysis shows that MFRS was better than LF in: length of surgery, blood loss, postsurgery Hb and patient length of stay (p<0,05. Conclusions: MFRS technique is an alternative technique of lumbar stenosis treatment better than the LF, in terms of improved levels of hsCRP and PGE2, leading to faster clinical outcomes improvement, less complications and lower costs. MFRS technique should be used as a treatment of lumbar stenosis.

Stress evolution and associated microstructure during transient creep of olivine at 1000-1200 °C

Science.gov (United States)

Thieme, M.; Demouchy, S.; Mainprice, D.; Barou, F.; Cordier, P.

2018-05-01

We study the mechanical response and correlated microstructure of axial deformed fine-grained olivine aggregates as a function of incremental finite strains. Deformation experiments were conducted in uniaxial compression in an internally heated gas-medium deformation apparatus at temperatures of 1000 and 1200 °C, at strain rates of 10-6 s-1 to 10-5 s-1 and at confining pressure of 300 MPa. Sample volumes are around 1.2 cm3. Finite strains range from 0.1 to 8.6% and corresponding maximal (final) differential stresses range from 80 to 1073 MPa for deformation at 1000 °C and from 71 to 322 MPa for deformation at 1200 °C. At 1200 °C, samples approach steady state deformation after about 8% of strain. At 1000 °C, significant strain hardening leads to stresses exceeding the confining pressure by a factor of 3.5 with brittle deformation after 3% of strain. Deformed samples were characterized by electron backscatter diffraction (EBSD) and transmission electron microscopy (TEM). EBSD maps with step sizes as low as 50 nm were acquired without introducing analytical artifacts for the first time. The grain size of deformed samples ranges from 2.1 to 2.6 μm. Despite clear strain hardening, texture or microstructure do not change as a function of stress or finite strain. This observation is supported by a constant texture strength (J-index) and symmetry (BA-index), constant grain shape and aspect ratio, constant density of geometrically necessary dislocations, grain orientation spread, and constant subgrain boundary spacing and misorientation in between samples. TEM shows that all samples exhibit unambiguous dislocation activity but with a highly heterogeneous dislocation distribution. Olivine grains display evidence of [1 0 0] and [0 0 1] slip activity, but there is no evidence of interaction between the dislocations from the different slip systems. Several observations of grain boundaries acting as dislocation sources have been found. We find no confirmation of

25 CFR 1200.17 - What special criteria will be used to evaluate management plans for judgment or settlement funds?

Science.gov (United States)

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false What special criteria will be used to evaluate management... FOR AMERICAN INDIANS, DEPARTMENT OF THE INTERIOR AMERICAN INDIAN TRUST FUND MANAGEMENT REFORM ACT Withdrawing Tribal Funds From Trust § 1200.17 What special criteria will be used to evaluate management plans...

Evaluation of the volatile profile of Tuber liyuanum by HS-SPME with GC-MS.

Science.gov (United States)

Liu, Changjiao; Li, Yu

2017-04-01

The volatile components of Tuber liyuanum were determined by HS-SPME with GC-MS for the first time. The effects of different fibre coating, extraction time, extraction temperature and sample amount were studied to get optimal extraction conditions. The optimal conditions were SPME fibre of Carboxen/PDMS, extraction time of 40 min, extraction temperature of 80 °C, sample amount of 2 g. Under these conditions 57 compounds in volatile of T. liyuanum were detected with a resemblance percentage above 80%. Aldehydes and aromatics were the main chemical families identified. The contribution of 3-Octanone(11.67%), phenylethyl alcohol (10.60%), isopentana (9.29%) and methylbutana (8.06%) for the total volatile profile were more significant in T. liyuanum than other compounds.

Serum GGT activity and hsCRP level in patients with type 2 diabetes mellitus with good and poor glycemic control: An evidence linking oxidative stress, inflammation and glycemic control.

Science.gov (United States)

Gohel, Mukesh G; Chacko, Anusha N

2013-12-20

Diabetes is undoubtedly one of the most challenging health problems in 21st century. Understanding the pathogenesis and preventing long term complications have been major goals of research in diabetes mellitus (DM). Research in the past few years has linked oxidative stress and inflammation to beta cell dysfunction. Aim of this study is to evaluate serum gamma-glutamyl transferase (GGT) activity (marker of oxidative stress) and high sensitivity C reactive protein (hsCRP) level (an inflammatory marker) in type 2 DM subjects with good and poor glycemic control. Further, we investigated correlation between serum GGT and hsCRP level with glycemic control (FBS, PP2BS, HbA1c) in subjects. A cross sectional study consists of 150 patients out of them 50 patients having type 2 DM with good control (Group II), 50 patients with type 2 DM with poor control (Group III) and 50 normal healthy control (Group I) were selected. Serum GGT, serum hsCRP, FBS, PP2BS, HbA1c, and other biochemical investigations include serum liver enzymes and lipids were measured. Mean serum GGT and hsCRP concentration were statistically significantly higher in group III patients compared to group I and group II subjects as well as increased in group II compared to group I (p stress and inflammation appears to be a key component and also associated with poor glycemic control and further pathogenesis of diabetes and its complications. All our finding suggesting a link between oxidative stress, inflammation and glycemic control in patient with type 2 diabetes mellitus.

Compressive Creep Behavior of NEXTEL(TradeMark) 720/Alumina Ceramic Matrix Composite at 1200 Degrees C in Air and in Steam Environment

National Research Council Canada - National Science Library

Szymczak, Neil R

2006-01-01

...) 720/Alumina ceramic matrix composite at 1200 deg. C in air and 100% steam environments. The effects of creep loading history on the tensile and compressive material behavior will also be examined...

Caseinophosphopeptides released after tryptic hydrolysis versus simulated gastrointestinal digestion of a casein-derived by-product.

Science.gov (United States)

Cruz-Huerta, E; García-Nebot, M J; Miralles, B; Recio, I; Amigo, L

2015-02-01

The production of caseinophosphopeptides from a casein-derived by-product generated during the manufacture of a functional ingredient based on antihypertensive peptides was attempted. The casein by-product was submitted to tryptic hydrolysis for 30, 60 and 120min and further precipitated with calcium chloride and ethanol at pH 4.0, 6.0 and 8.0. Identification and semi quantification of the derived products by tandem mass spectrometry revealed some qualitative and quantitative changes in the released caseinophosphopeptides over time at the different precipitation pHs. The by-product was also subjected to simulated gastrointestinal digestion. Comparison of the resulting peptides showed large sequence homology in the phosphopeptides released by tryptic hydrolysis and simulated gastrointestinal digestion. Some regions, specifically αS1-CN 43-59, αS1-CN 60-74, β-CN 1-25 and β-CN 30-50 showed resistance to both tryptic hydrolysis and simulated digestion. The results of the present study suggest that this casein-derived by-product can be used as a source of CPPs. Copyright © 2014 Elsevier Ltd. All rights reserved.

Effects of Fire Suppression Agents and Weathering in the Analysis of Fire Debris by HS-MS eNose

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Barbara Falatová

2018-06-01

Full Text Available In arson attacks the detection of ignitable liquid residues (ILRs at fire scenes provides key evidence since ignitable liquids, such as gasoline, are commonly used to initiate the fire. In most forensic laboratories gas chromatography-mass spectrometry is employed for the analysis of ILRs. When a fire occurs, suppression agents are used to extinguish the fire and, before the scene is investigated, the samples at the scene are subjected to a variety of processes such as weathering, which can significantly modify the chemical composition and thus lead to erroneous conclusions. In order to avoid this possibility, the application of chemometric tools that help the analyst to extract useful information from data is very advantageous. The study described here concerned the application of a headspace-mass spectrometry electronic nose (HS-MS eNose combined with chemometric tools to determine the presence/absence of gasoline in weathered fire debris samples. The effect of applying two suppression agents (Cafoam Aquafoam AF-6 and Pyro-chem PK-80 Powder and delays in the sampling time (from 0 to 48 h were studied. It was found that, although the suppression systems affect the mass spectra, the HS-MS eNose in combination with suitable pattern recognition chemometric tools, such as linear discriminant analysis, is able to identify the presence of gasoline in any of the studied situations (100% correct classification.

Determination of organotin compounds by headspace solid-phase microextraction-gas chromatography-pulsed flame-photometric detection (HS-SPME-GC-PFPD)

Energy Technology Data Exchange (ETDEWEB)

Bravo, Manuel [Universite de Pau et des Pays de L' Adour, Laboratoire de Chimie Analytique, LCABIE, UMR CNRS 5034, Pau (France); Pontificia Universidad Catolica de Valparaiso, Laboratorio de Quimica Analitica y Ambiental, Instituto de Quimica, Valparaiso (Chile); Lespes, Gaetane; Gautier, Martine Potin [Pontificia Universidad Catolica de Valparaiso, Laboratorio de Quimica Analitica y Ambiental, Instituto de Quimica, Valparaiso (Chile); Gregori, Ida de; Pinochet, Hugo [Universite de Pau et des Pays de L' Adour, Laboratoire de Chimie Analytique, LCABIE, UMR CNRS 5034, Pau (France)

2005-12-01

A method based on Headspace solid-phase microextraction (HS-SPME, with a 100 {mu}m PDMS-fiber) in combination with gas-chromatography and pulsed flame-photometric detection (GC-PFPD) has been investigated for simultaneous determination of eight organotin compounds. Monobutyltin (MBT), dibutyltin (DBT), tributyltin (TBT), monophenyltin (MPhT), and the semi-volatile diphenyltin (DPhT), triphenyltin (TPhT), monooctyltin (MOcT), and dioctyltin (DOcT) were determined after derivatization with sodium tetraethylborate. The conditions used for the extraction and preconcentration step were optimised by experimental design methodology. Tripropyltin (TPrT) and diheptyltin (DHepT) were used as internal standards for quantification of volatile and semi-volatile organotin compounds, respectively. The analytical precision (RSD) for ten successive injections of a standard mixture containing all the organic tin compounds ranged between 2 and 11%. The limits of detection for all the organotin compounds were sub ng (Sn) L{sup -1} in water and close to ng (Sn) kg{sup -1} in sediments. The accuracy of the method was evaluated by analysis of two certified reference material (CRM) sediment samples. The HS-SPME-GC-PFPD was then applied to the analysis of three harbour sediment samples. The results showed that headspace SPME is an attractive tool for analysis of organotin compounds in solid environmental matrices. (orig.)

Human β-globin locus control region: Analysis of the 5' DNase I hypersensitive site HS 2 in transgenic mice

International Nuclear Information System (INIS)

Caterina, J.J.; Ryan, T.M.; Pawlik, K.M.; Townes, T.M.; Brinster, R.L.; Behringer, R.R.; Palmiter, R.D.

1991-01-01

The human β-globin locus control region (LCR) is essential for high-level expression of human var-epsilon-, γ-, and β-globin genes. Developmentally stable DNase I hypersensitive sites (designated HS) mark sequences within this region that are important for LCR activity. A 1.9-kilobase (kb) fragment containing the 5' HS 2 site enhances human β-globin gene expression 100-fold in transgenic mice and also confers position-independent expression. To further define important sequences within this region, deletion mutations of the 1.9-kb fragment were introduced upstream of the human β-globin gene, and the constructs were tested for activity in transgenic mice. Although enhancer activity was gradually lost with deletion of both 5' and 3' sequences, a 373-base-pair (BP) fragment retained the ability to confer relative position-independent expression. Three prominent DNase I footprints were observed in this region with extracts from the human erythroleukemia cell line K-562, one of which contained duplicated binding sites for transcription factor AP-1 (activator protein 1). When the 1.9-kb fragment containing an 19-bp deletion of the AP-1 binding sites was tested in transgenic mice, enhancer activity decreased 20-fold but position-independent expression was retained

Fibroblast Growth Factor 21 (FGF21, Free Fatty Acid (FFA, High Sensitivity C-reactive Protein (hsCRP and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR Among Indonesian Obese Non-Diabetic Males

Directory of Open Access Journals (Sweden)

Yani Lina

2009-12-01

Full Text Available BACKGROUND: Fibroblast growth factor-21 (FGF21 is known as an important endocrine and paracrine regulator of metabolic homeostasis. Recent studies have shown that FGF21 attenuates lipolysis in human adipocytes, which is suggested as a FGF21's mechanism as anti-hyperlipidemia, anti-hyperglycemia and anti-obesity. The aim of this study was to measure the correlation between FGF21, FFA, hsCRP and HOMA-IR among Indonesian obese non diabetic males. METHODS: The study was observational with cross sectional design. The analysis was done in 137 subjects aged 30-60 years with non diabetic abdominal obesity. We measured the biochemical markers FGF21, FFA, hsCRP, fasting insulin and fasting glucose. We also measured weight, height, waist circumrefence (WC, creatinine, serum glutamin oxaloacetic transaminase (SGOT, and serum glutamic pyruvic transaminase (SGPT, systolic blood pressure (SBP and diastolic blood pressure (DBP. Correlation between markers was measured using Pearson and Spearman's analysis. RESULTS: There were significant positive correlations between FGF21-HOMA-IR (r=0.314, p=0.000; FGF21-WC (r=0.173, p=0.043; FFA=hsCRP r=0.270, p=0.001; and WC-HOMA-IR (r=0.279, p=0.001. There was significant negative correlation between FGF21-FFA (r=-0.038, p=0.657 and FGF21-hsCRP (r=-0.061, p=0.482. CONCLUSIONS: In this study we found that although there was no significant correlation, FGF21 might act as an anti-lipolytic and anti-inflammation agent among Indonesian obese non-diabetic males. Our findings agree with results of previous studies that the positive correlation between FGF21-WC and FGF21-HOMA-IR might occur as a compensatory mechanism or resistance to FGF21 in obesity. KEYWORDS: obesity, FGF21, FFA, hsCRP, HOMA-IR.

Anti-tumor activity of a novel HS-mimetic-vascular endothelial growth factor binding small molecule.

Directory of Open Access Journals (Sweden)

Kazuyuki Sugahara

Full Text Available The angiogenic process is controlled by variety of factors of which the vascular endothelial growth factor (VEGF pathway plays a major role. A series of heparan sulfate mimetic small molecules targeting VEGF/VEGFR pathway has been synthesized. Among them, compound 8 (2-butyl-5-chloro-3-(4-nitro-benzyl-3H-imidazole-4-carbaldehyde was identified as a significant binding molecule for the heparin-binding domain of VEGF, determined by high-throughput-surface plasmon resonance assay. The data predicted strong binding of compound 8 with VEGF which may prevent the binding of VEGF to its receptor. We compared the structure of compound 8 with heparan sulfate (HS, which have in common the functional ionic groups such as sulfate, nitro and carbaldehyde that can be located in similar positions of the disaccharide structure of HS. Molecular docking studies predicted that compound 8 binds at the heparin binding domain of VEGF through strong hydrogen bonding with Lys-30 and Gln-20 amino acid residues, and consistent with the prediction, compound 8 inhibited binding of VEGF to immobilized heparin. In vitro studies showed that compound 8 inhibits the VEGF-induced proliferation migration and tube formation of mouse vascular endothelial cells, and finally the invasion of a murine osteosarcoma cell line (LM8G7 which secrets high levels of VEGF. In vivo, these effects produce significant decrease of tumor burden in an experimental model of liver metastasis. Collectively, these data indicate that compound 8 may prevent tumor growth through a direct effect on tumor cell proliferation and by inhibition of endothelial cell migration and angiogenesis mediated by VEGF. In conclusion, compound 8 may normalize the tumor vasculature and microenvironment in tumors probably by inhibiting the binding of VEGF to its receptor.

Characterization of the B-chain of human plasma α2HS-glycoprotein. The complete amino acid sequence and primary structure of its heteroglycan

NARCIS (Netherlands)

Vliegenthart, J.F.G.; Gejyo, F.; Chang, J.-L.; Bürgi, W.; Schmid, K.; Offner, G.D.; Troxler, R.F.; Halbeek, H. van

1983-01-01

α2HS-Glycoprotein, a normal human plasma protein, was recently shown to consist of two polypeptide chains. In the present study, we have separated these two chains from one another and have elucidated the complete primary structure of the B-chain. Employing automated Edman degradation, the

Maps of surface activity of 137Cs of Slovakia on scale 1:200 000

International Nuclear Information System (INIS)

Gluch, A.

2005-05-01

The present set of maps (13 maps) arose from the geological project 'Re-ambulation of 137 Cs radioactivity map of Slovakia at scales 1:200 000 and 1:500 000' in phase of indicative geological survey of environmental factors. Maps document the state of contamination of the territory of Slovakia by one of the radioisotopes cesium-137 at the reference date 01.01.2005. In solving of geological tasks were used all available relevant data on measurements of 137 Cs activity from the whole territory of the Slovak Republic for the period from 1990 to 2003 from results of air and ground gamma spectrometric measurements. (authors)

Reply to "On Vaporization of liquid Pb-Li eutectic alloy from 1000 K to 1200 K- A high temperature mass spectrometric study"

Science.gov (United States)

Jain, Uttam; Mukherjee, Abhishek

2018-03-01

This communication is in response to a letter to editor commenting on the authors' earlier paper "Vaporization of liquid Pb-Li eutectic alloy from 1000 K to 1200 K - A high temperature mass spectrometric study".

Celecoxib and tauro-ursodeoxycholic acid co-treatment inhibits cell growth in familial adenomatous polyposis derived LT97 colon adenoma cells

Energy Technology Data Exchange (ETDEWEB)

Heumen, Bjorn W.H. van, E-mail: b.vanheumen@mdl.umcn.nl [Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Roelofs, Hennie M.J.; Morsche, Rene H.M. te [Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Marian, Brigitte [Institute of Cancer Research, Wien University, Vienna (Austria); Nagengast, Fokko M.; Peters, Wilbert H.M. [Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands)

2012-04-15

Chemoprevention would be a desirable strategy to avoid duodenectomy in patients with familial adenomatous polyposis (FAP) suffering from duodenal adenomatosis. We investigated the in vitro effects on cell proliferation, apoptosis, and COX-2 expression of the potential chemopreventives celecoxib and tauro-ursodeoxycholic acid (UDCA). HT-29 colon cancer cells and LT97 colorectal micro-adenoma cells derived from a patient with FAP, were exposed to low dose celecoxib and UDCA alone or in combination with tauro-cholic acid (CA) and tauro-chenodeoxycholic acid (CDCA), mimicking bile of FAP patients treated with UDCA. In HT-29 cells, co-treatment with low dose celecoxib and UDCA resulted in a decreased cell growth (14-17%, p < 0.01). A more pronounced decrease (23-27%, p < 0.01) was observed in LT97 cells. Cell growth of HT-29 cells exposed to 'artificial bile' enriched with UDCA, was decreased (p < 0.001), either in the absence or presence of celecoxib. In LT97 cells incubated with 'artificial bile' enriched with UDCA, cell growth was decreased only in the presence of celecoxib (p < 0.05). No clear evidence was found for involvement of proliferating cell nuclear antigen, caspase-3, or COX-2 in the cellular processes leading to the observed changes in cell growth. In conclusion, co-treatment with low dose celecoxib and UDCA has growth inhibitory effects on colorectal adenoma cells derived from a patient with FAP, and further research on this combination as promising chemopreventive strategy is desired. -- Highlights: Black-Right-Pointing-Pointer Celecoxib and UDCA acid co-treatment decreases cell growth in colon tumor cells. Black-Right-Pointing-Pointer UDCA enriched 'artificial bile' decreases LT-97 cell growth only in presence of celecoxib. Black-Right-Pointing-Pointer PCNA, caspase-3, nor COX-2 seem to be involved in the observed changes in cell growth.

Celecoxib and tauro-ursodeoxycholic acid co-treatment inhibits cell growth in familial adenomatous polyposis derived LT97 colon adenoma cells

International Nuclear Information System (INIS)

Heumen, Bjorn W.H. van; Roelofs, Hennie M.J.; Morsche, René H.M. te; Marian, Brigitte; Nagengast, Fokko M.; Peters, Wilbert H.M.

2012-01-01

Chemoprevention would be a desirable strategy to avoid duodenectomy in patients with familial adenomatous polyposis (FAP) suffering from duodenal adenomatosis. We investigated the in vitro effects on cell proliferation, apoptosis, and COX-2 expression of the potential chemopreventives celecoxib and tauro-ursodeoxycholic acid (UDCA). HT-29 colon cancer cells and LT97 colorectal micro-adenoma cells derived from a patient with FAP, were exposed to low dose celecoxib and UDCA alone or in combination with tauro-cholic acid (CA) and tauro-chenodeoxycholic acid (CDCA), mimicking bile of FAP patients treated with UDCA. In HT-29 cells, co-treatment with low dose celecoxib and UDCA resulted in a decreased cell growth (14–17%, p < 0.01). A more pronounced decrease (23–27%, p < 0.01) was observed in LT97 cells. Cell growth of HT-29 cells exposed to ‘artificial bile’ enriched with UDCA, was decreased (p < 0.001), either in the absence or presence of celecoxib. In LT97 cells incubated with ‘artificial bile’ enriched with UDCA, cell growth was decreased only in the presence of celecoxib (p < 0.05). No clear evidence was found for involvement of proliferating cell nuclear antigen, caspase-3, or COX-2 in the cellular processes leading to the observed changes in cell growth. In conclusion, co-treatment with low dose celecoxib and UDCA has growth inhibitory effects on colorectal adenoma cells derived from a patient with FAP, and further research on this combination as promising chemopreventive strategy is desired. -- Highlights: ► Celecoxib and UDCA acid co-treatment decreases cell growth in colon tumor cells. ► UDCA enriched ‘artificial bile’ decreases LT-97 cell growth only in presence of celecoxib. ► PCNA, caspase-3, nor COX-2 seem to be involved in the observed changes in cell growth.

Absolute hypoxic exercise training enhances in vitro thrombin generation by increasing procoagulant platelet-derived microparticles under high shear stress in sedentary men.

Science.gov (United States)

Chen, Yu-Wen; Chen, Yi-Ching; Wang, Jong-Shyan

2013-05-01

HS (high shear) stress associated with artery stenosis facilitates TG (thrombin generation) by increasing the release of procoagulant PDMPs (platelet-derived microparticles). Physical exercise and hypoxia may paradoxically modulate vascular thrombotic risks. The aim of the present study was to investigate how exercise training with/without hypoxia affected TG mediated by PDMPs under physio-pathological shear flows. A total of 75 sedentary males were randomly divided into five groups (n=15 in each group): 21% O2 [NC (normoxic control)] or 15% O2 [HC (hypoxic control)] at rest or were trained at 50% of peak work rate under 21% O2 [NT (normoxic training)] or 15% O2 [HAT (hypoxic-absolute training)], or 50% of HR (heart rate) reserve under 15% O2 [HRT (hypoxic-relative training)] for 30 min/day, 5 days/week for 4 weeks. The PDMP characteristics and dynamic TG were measured by flow cytometry and thrombinography respectively. Before the intervention, strenuous exercise markedly increased the PDMP count (14.8%) and TG rate (19.5%) in PDMP-rich plasma at 100 dynes/cm2 of shear stress (Pexercise. Conversely, HAT notably promoted the PDMP count (37.3%) and TG rate (38.9%) induced by HS (Pexercise. We conclude that both HRT and NT depress similarly HS-mediated TG during exercise, but HAT accelerates the prothrombotic response to vigorous exercise. These findings provide new insights into how exercise training under a hypoxic condition influences the risk of thrombosis associated with stenotic arteries.

Solid-state phase relationships in the calcia-titania-zirconia system at 1200 C

International Nuclear Information System (INIS)

Swenson, D.; Nieh, T.G.; Fournelle, J.H.

1998-01-01

Phase relationships were investigated in the CaO-TiO 2 -ZrO 2 system at 1200 C for compositions containing 2 O 7 ) and calzirtite (Ca 2 Zr 5 Ti 2 O 16 ), was confirmed. Each of these phases exhibited a significant range of homogeneity between TiO 2 and ZrO 2 , while maintaining a nearly constant concentration of CaO. The ternary solubilities of the constituent binary phases were found to be small (typically 3 and CaZrO 3 ). These latter phases displayed mutual solubilities of at least 22 mol% but exhibited significant variations in composition from grain to grain. Thermodynamic equilibrium was clearly not established in several samples, although most of the phase relationship information obtained was self-consistent

A cluster-randomized controlled knowledge translation feasibility study in Alberta community pharmacies using the PARiHS framework: study protocol.

Science.gov (United States)

Rosenthal, Meagen M; Tsuyuki, Ross T; Houle, Sherilyn Kd

2015-01-01

Despite evidence of benefit for pharmacist involvement in chronic disease management, the provision of these services in community pharmacy has been suboptimal. The Promoting Action on Research Implementation in Health Services (PARiHS) framework suggests that for knowledge translation to be effective, there must be evidence of benefit, a context conducive to implementation, and facilitation to support uptake. We hypothesize that while the evidence and context components of this framework are satisfied, that uptake into practice has been insufficient because of a lack of facilitation. This protocol describes the rationale and methods of a feasibility study to test a facilitated pharmacy practice intervention based on the PARiHS framework, to assist community pharmacists in increasing the number of formal and documented medication management services completed for patients with diabetes, dyslipidemia, and hypertension. A cluster-randomized before-after design will compare ten pharmacies from within a single organization, with the unit of randomization being the pharmacy. Pharmacies will be randomized to facilitated intervention based on the PARiHS framework or usual practice. The Alberta Context Tool will be used to establish the context of practice in each pharmacy. Pharmacies randomized to the intervention will receive task-focused facilitation from an external facilitator, with the goal of developing alternative team processes to allow the greater provision of medication management services for patients with diabetes, hypertension, and dyslipidemia. The primary outcome will be a process evaluation of the needs of community pharmacies to provide more clinical services, the acceptability and uptake of modifications made, and the willingness of pharmacies to participate. Secondary outcomes will include the change in the number of formal and documented medication management services in the aforementioned chronic conditions provided 6 months before, versus after, the

Capmul MCM/Solutol HS15-Based Microemulsion for Enhanced Oral Bioavailability of Rebamipide.

Science.gov (United States)

Kim, Ki Taek; Lee, Jae-Young; Park, Ju-Hwan; Cho, Hyun-Jong; Yoon, In-Soo; Kim, Dae-Duk

2017-04-01

Rebamipide (RBP) is a potent anti-ulcer and anti-oxidative agent, which is a BCS class IV drug with a low oral bioavailability of less than 10%. Thus, the systemic absorption of RBP into the blood circulation is an essential prerequisite for exerting its pharmacological activities after oral dosing. Herein, we report on microemulsion (ME) systems for the enhancement of oral RBP bioavailability. In this study, MEs consisting of Capmul MCM (oil), Solutol HS15 (surfactant), and ethanol (co-surfactant) were prepared by the construction of pseudo-ternary phase diagram. The RBP-loaded MEs had spherical nano-sized droplets with narrow size distribution and neutral zeta potential. Moreover, the prepared MEs significantly enhanced the dissolution and oral bioavailability of RBP with no discernible intestinal toxicity. These results suggest that the present ME system could be further developed as an alternative oral formulation for RBP.

The Effect of 8 Weeks of Aerobic Training and Consumption of Hydro-alcoholic Extract of Nettle on Apelin and hs-CRP plasma Levels of Overweight and Obese Women

Directory of Open Access Journals (Sweden)

M Madadi Jaberi

2016-12-01

Full Text Available Background and aim: The use of exercise along with herbal supplements is one method proposed for controlling obesity and its complications. The aim of this study was to investigate the effect of 8 weeks aerobic training and use of hydro-alcoholic extract of nettle on levels apelin and hs-CRP plasma in overweight and obese women. Methods: The present quasi-experimental study was conducted with blind randomized clinical trial. 46 overweight and obese women (body mass index greater than 25 kilograms per square millimeter two, aged 25-45 years were selected purposefully and randomly divided into four groups of: aerobic training + hydro alcoholic extract of nettle, aerobic exercise + placebo extract of nettle and placebo. The intervention group and placebo received 8 mg of hydro alcoholic extract of nettle 8 ml of water-soluble daily for 8 weeks respectively. Aerobic exercise ergometer for 8 weeks, 3 sessions of 16 to 30 minutes with the intensity of 60-75% heart rate was reserved. In two pre and post-test after 14 hours of fasting at the same conditions, blood samples were collected. The ELISA method was use to assess levels of plasma apelin and hs-CRP d. Data obtained were analyzed using the Kolmogorov-Smirnov test, ANOVA, t-test and LSD test. Results: The results showed that the levels of hs-CRP were significantly different in comparison among the groups as well as in groups of aerobic exercise + hydro alcoholic extract of nettle, nettle and hydro-alcholic aerobic exercise + placebo significant reduction was observed (p>0.05. Conclusion: It seems that consumption of Nettle extract along with aerobic exercise through Weight loss, body fat percentage and BMI, play an effective role in control of obesity and reducing of inflammatory Apelin markers and hs-CRP in obese women

Structure-Based Drug Design of Small Molecule Peptide Deformylase Inhibitors to Treat Cancer

Directory of Open Access Journals (Sweden)

Jian Gao

2016-03-01

Full Text Available Human peptide deformylase (HsPDF is an important target for anticancer drug discovery. In view of the limited HsPDF, inhibitors were reported, and high-throughput virtual screening (HTVS studies based on HsPDF for developing new PDF inhibitors remain to be reported. We reported here on diverse small molecule inhibitors with excellent anticancer activities designed based on HTVS and molecular docking studies using the crystal structure of HsPDF. The compound M7594_0037 exhibited potent anticancer activities against HeLa, A549 and MCF-7 cell lines with IC50s of 35.26, 29.63 and 24.63 μM, respectively. Molecular docking studies suggested that M7594_0037 and its three derivatives could interact with HsPDF by several conserved hydrogen bonds. Moreover, the pharmacokinetic and toxicity properties of M7594_0037 and its derivatives were predicted using the OSIRIS property explorer. Thus, M7594_0037 and its derivatives might represent a promising scaffold for the further development of novel anticancer drugs.

Interactions of Solutol HS 15 and Cremophor EL with plasma lipoproteins.

Science.gov (United States)

Woodburn, K; Sykes, E; Kessel, D

1995-07-01

Two emulsifying agents, Solutol HS15 and Cremophor EL, were compared with regard to their effects on human plasma lipoproteins in vitro and on mouse plasma lipoproteins in vitro and in vivo. Both agents promoted binding of a hydrophobic photosensitizing agent (C8KC) to a circulating plasma species of low bouyant density. Persistence of this material was greater with Cremophor than with Solutol. Experiments carried out with labeled Solutol indicated that the vehicle itself is a component of this new species. High concentrations of either vehicle ( > or = 0.06%) led to decreased electrophoretic mobility of human LDL and HDL in vitro. In the mouse, a different effect was observed, resulting in complex changes in electrophoretic mobility of plasma lipoproteins. The plasma half-life of C8KC in the circulation of the mouse was correlated with the persistence of an altered electrophoretic lipoprotein pattern. Since Solutol and C8KC showed similar half-lives, this result suggests that the plasma half-life of the sensitizer is correlated with the persistence of the vehicle. While Solutol and Cremophor were designed to be vehicles for drug formulation, they also influence persistence of some drugs in the circulation.

O-Succinyl-L-homoserine-based C4-chemical production: succinic acid, homoserine lactone, γ-butyrolactone, γ-butyrolactone derivatives, and 1,4-butanediol.

Science.gov (United States)

Hong, Kuk-Ki; Kim, Jeong Hyun; Yoon, Jong Hyun; Park, Hye-Min; Choi, Su Jin; Song, Gyu Hyeon; Lee, Jea Chun; Yang, Young-Lyeol; Shin, Hyun Kwan; Kim, Ju Nam; Cho, Kyung Ho; Lee, Jung Ho

2014-10-01

There has been a significant global interest to produce bulk chemicals from renewable resources using engineered microorganisms. Large research programs have been launched by academia and industry towards this goal. Particularly, C4 chemicals such as succinic acid (SA) and 1,4-butanediol have been leading the path towards the commercialization of biobased technology with the effort of replacing chemical production. Here we present O-Succinyl-L-homoserine (SH) as a new, potentially important platform biochemical and demonstrate its central role as an intermediate in the production of SA, homoserine lactone (HSL), γ-butyrolactone (GBL) and its derivatives, and 1,4-butanediol (BDO). This technology encompasses (1) the genetic manipulation of Escherichia coli to produce SH with high productivity, (2) hydrolysis into SA and homoserine (HS) or homoserine lactone hydrochloride, and (3) chemical conversion of either HS or homoserine lactone HCL (HSL·HCl) into drop-in chemicals in polymer industry. This production strategy with environmental benefits is discussed in the perspective of targeting of fermented product and a process direction compared to petroleum-based chemical conversion, which may reduce the overall manufacturing cost.

Single-Event Effect Testing of the Cree C4D40120D Commercial 1200V Silicon Carbide Schottky Diode

Science.gov (United States)

Lauenstein, J.-M.; Casey, M. C.; Wilcox, E. P.; Kim, Hak; Topper, A. D.

2014-01-01

This study was undertaken to determine the single event effect (SEE) susceptibility of the commercial silicon carbide 1200V Schottky diode manufactured by Cree, Inc. Heavy-ion testing was conducted at the Texas A&M University Cyclotron Single Event Effects Test Facility (TAMU). Its purpose was to evaluate this device as a candidate for use in the Solar-Electric Propulsion flight project.

Examining the utility of satellite-based wind sheltering estimates for lake hydrodynamic modeling

Science.gov (United States)

Van Den Hoek, Jamon; Read, Jordan S.; Winslow, Luke A.; Montesano, Paul; Markfort, Corey D.

2015-01-01

Satellite-based measurements of vegetation canopy structure have been in common use for the last decade but have never been used to estimate canopy's impact on wind sheltering of individual lakes. Wind sheltering is caused by slower winds in the wake of topography and shoreline obstacles (e.g. forest canopy) and influences heat loss and the flux of wind-driven mixing energy into lakes, which control lake temperatures and indirectly structure lake ecosystem processes, including carbon cycling and thermal habitat partitioning. Lakeshore wind sheltering has often been parameterized by lake surface area but such empirical relationships are only based on forested lakeshores and overlook the contributions of local land cover and terrain to wind sheltering. This study is the first to examine the utility of satellite imagery-derived broad-scale estimates of wind sheltering across a diversity of land covers. Using 30 m spatial resolution ASTER GDEM2 elevation data, the mean sheltering height, hs, being the combination of local topographic rise and canopy height above the lake surface, is calculated within 100 m-wide buffers surrounding 76,000 lakes in the U.S. state of Wisconsin. Uncertainty of GDEM2-derived hs was compared to SRTM-, high-resolution G-LiHT lidar-, and ICESat-derived estimates of hs, respective influences of land cover type and buffer width on hsare examined; and the effect of including satellite-based hs on the accuracy of a statewide lake hydrodynamic model was discussed. Though GDEM2 hs uncertainty was comparable to or better than other satellite-based measures of hs, its higher spatial resolution and broader spatial coverage allowed more lakes to be included in modeling efforts. GDEM2 was shown to offer superior utility for estimating hs compared to other satellite-derived data, but was limited by its consistent underestimation of hs, inability to detect within-buffer hs variability, and differing accuracy across land cover types. Nonetheless

Urban Transformation and Property Value Variation. The Role of HS Stations

Directory of Open Access Journals (Sweden)

Carmela Gargiulo

2010-04-01

operation, partly in operation or not yet in operation. Besides, the description of each case is divided into: summary description of the characteristics of the HS railway line; description of the main features of cities that represent the nodes of the selected routes; identify the characteristics of the surrounding context of the railway station; measurement of the change in property values in the surrounding context of the railway station due to HS link. The third part provides a comparative outline of the effects of High Speed stations on property values and the urban typologies and characteristics that influence this relation. The first result of this article is a comparative framework between all study cases. In conclusion, the comparative reading shows that the effect from opening of the High Speed railway stations on property values is valuable in cases where it contributes significantly to raising the socio-economic and the quality conditions of spaces.

Potential of cancer cell-derived exosomes in clinical application: a review of recent research advances.

Science.gov (United States)

Sun, Yu; Liu, Jing

2014-06-01

Exosomes are 30- to 100-nm, membrane-bound vesicles that are released by most types of cells, including tumor cells. Exosomes contain a great variety of bioactive molecules, including signal peptides, microRNA, lipids, and DNA. In cancer, tumor cells aberrantly secrete large quantities of exosomes to transport paracrine signals or to contribute to tumor-environment interaction at a distance. The goal of this review was to discuss the recent advances on the mechanism of cancer-derived exosomes in tumor regulation. Pertinent articles and abstracts were identified through searches of PubMed for literature published from 1983 to December 2013. Search terms included exosome, tumor, cancer, diagnosis, and therapy. All of the exposed evidence points to communication between cancer cells and their surroundings, either mediated by cancer cell-derived exosomes or by stromal cell-derived exosomes. This communication probably supports tumor proliferation, motility, invasion, angiogenesis, and premetastatic niche preparation. In addition, recent research implies that cancer cell-derived exosomes play a suppressive role in cancer-directed immune response. The biomarkers detected in bodily fluid-derived exosomes imply a potential for exosomes in cancer diagnosis. Also, exosomes could be used as a vehicle to selectively deliver therapeutic nucleic-acid drugs or conventional drugs for tumor therapy. The tolerability and feasibility of cancer exosomes in diagnosis and therapy need to be further evaluated. Copyright © 2014 Elsevier HS Journals, Inc. All rights reserved.

A randomized controlled clinical trial investigating the effect of calcium supplement plus low-dose aspirin on hs-CRP, oxidative stress and insulin resistance in pregnant women at risk for pre-eclampsia.

Science.gov (United States)

Asemi, Z; Samimi, M; Heidarzadeh, Z; Khorrammian, H; Tabassi, Z

2012-05-15

Increased levels of pro-inflammatory factors, markers of oxidative stress and insulin resistance during pregnancy have been associated with the development of pre-eclampsia. There is some evidence to suggest that calcium supplement and aspirin can reduce the risk of the disorder. To our knowledge, no reports are available indicating the effects of consumed calcium supplement plus aspirin on high sensitivity C-reactive protein (hs-CRP), oxidative stress parameters and insulin resistance in pregnant women at risk for pre-eclampsia. This study was designed to investigate the effects of consumed calcium supplement plus low-dose aspirin on hs-CRP, oxidative stress parameters and insulin resistance among Iranian pregnant women at risk for pre-eclampsia. This randomized single-blind controlled clinical trial was carried out among 42 pregnant women at risk for pre-eclampsia, primigravida, aged 18-40 year old who were carrying singleton pregnancy at their third trimester. Subjects were randomly assigned to received either the placebo (n = 22) or calcium supplement plus low-dose aspirin (n = 20) for 9 weeks. Calcium supplement plus low-dose aspirin were containing 500 mg carbonate calcium plus 80 mg aspirin. Fasting blood samples were taken at baseline and after 9 weeks intervention to measure serum hs-CRP, oxidative stress parameters including plasma Total Antioxidant Capacity (TAC) and Total Glutathione (GSH), Fasting Plasma Glucose (FPG), serum insulin and HOMA-IR score. Consumption of calcium supplement plus low-dose aspirin resulted in a significant difference serum hs-CRP levels as compared to the placebo (102.87 vs. 3227.75 ng mL(-1), p = 0.01). Also, mean changes for plasma TAC (68.96 vs. -74.46 mmol L(-1), p = 0.04) and total GSH levels (304.33 vs. -39.33 micromol L(-1), p = 0.03) were significantly different between the two groups. No significant differences were found comparing calcium supplement plus low-dose aspirin and placebo in terms of their effects on FPG

Blood hsCRP And PGE2 Content With Clinical Outcome Using Modified Fenestrat Restorative Spinoplasty Better Than Lamonectomy-Fusion In Lumbar Stenosis

OpenAIRE

T Mahadewa; Sri Maliawan; A Raka-Sudewi; M Wiryana

2012-01-01

Objective: Modified Fenestration-Restorative Spinoplasty (MFRS) technique is an alternative to lumbar stenosis treatment, providing the equal decompression comparing with laminectomy techniques, without the implant, less expensive and complication rates. The purpose of this study was to determine which technique gives better inflammation and clinical outcome based on high sensitive C-Reactive Protein biomarker (hsCRP) and Prostaglandin E2 (PGE 2), Visua...

Chitosan and Its Derivatives for Application in Mucoadhesive Drug Delivery Systems

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Twana Mohammed M. Ways

2018-03-01

Full Text Available Mucoadhesive drug delivery systems are desirable as they can increase the residence time of drugs at the site of absorption/action, provide sustained drug release and minimize the degradation of drugs in various body sites. Chitosan is a cationic polysaccharide that exhibits mucoadhesive properties and it has been widely used in the design of mucoadhesive dosage forms. However, its limited mucoadhesive strength and limited water-solubility at neutral and basic pHs are considered as two major drawbacks of its use. Chemical modification of chitosan has been exploited to tackle these two issues. In this review, we highlight the up-to-date studies involving the synthetic approaches and description of mucoadhesive properties of chitosan and chitosan derivatives. These derivatives include trimethyl chitosan, carboxymethyl chitosan, thiolated chitosan, chitosan-enzyme inhibitors, chitosan-ethylenediaminetetraacetic acid (chitosan-EDTA, half-acetylated chitosan, acrylated chitosan, glycol chitosan, chitosan-catechol, methyl pyrrolidinone-chitosan, cyclodextrin-chitosan and oleoyl-quaternised chitosan. We have particularly focused on the effect of chemical derivatization on the mucoadhesive properties of chitosan. Additionally, other important properties including water-solubility, stability, controlled release, permeation enhancing effect, and in vivo performance are also described.

Development of Ion-Plasma Coatings for Protecting Intermetallic Refractory Alloys VKNA-1V and VKNA-25 in the Temperature Range of 1200 - 1250°C

Science.gov (United States)

Budinovskii, S. A.; Matveev, P. V.; Smirnov, A. A.

2017-05-01

Multilayer heat-resistant ion-plasma coatings for protecting the parts of the hot duct of gas-turbine engines produced from refractory nickel alloys based on VKNA intermetallics from high-temperature oxidation are considered. Coatings of the Ni - Cr - Al (Ta, Re, Hf, Y) + Al - Ni - Y systems are tested for high-temperature strength at 1200 and 1250°C. Metallographic and microscopic x-ray spectrum analyses of the structure and composition of the coatings in the initial condition and after the testing are performed. The effect of protective coatings of the Ni - Cr - Al - Hf + Al - Ni - Y systems on the long-term strength of alloys VKNA-1V and VKNA-25 at 1200°C is studied.

An improved harmony search algorithm for synchronization of discrete-time chaotic systems

International Nuclear Information System (INIS)

Santos Coelho, Leandro dos; Andrade Bernert, Diego Luis de

2009-01-01

The harmony search (HS) algorithm is a recently developed meta-heuristic algorithm, and has been very successful in a wide variety of optimization problems. HS was conceptualized using an analogy with music improvisation process where music players improvise the pitches of their instruments to obtain better harmony. The HS algorithm does not require initial values and uses a random search instead of a gradient search, so derivative information is unnecessary. Furthermore, the HS algorithm is simple in concept, few in parameters, easy in implementation, imposes fewer mathematical requirements, and does not require initial value settings of the decision variables. In recent years, the investigation of synchronization and control problem for discrete chaotic systems has attracted much attention, and many possible applications. The tuning of a proportional-integral-derivative (PID) controller based on an improved HS (IHS) algorithm for synchronization of two identical discrete chaotic systems subject the different initial conditions is investigated in this paper. Simulation results of the IHS to determine the PID parameters to synchronization of two Henon chaotic systems are compared with other HS approaches including classical HS and global-best HS. Numerical results reveal that the proposed IHS method is a powerful search and controller design optimization tool for synchronization of chaotic systems.

Experimental study of high-energy resolution lead/scintillating fiber calorimetry in the 600-1200 MeV energy region

International Nuclear Information System (INIS)

Bellini, V.; Bianco, S.; Capogni, M.; Casano, L.; D'Angelo, A.; Fabbri, F.L.; Ghio, F.; Giardoni, M.; Girolami, B.; Hu, L.; Levi Sandri, P.; Moricciani, D.; Nobili, G.; Passamonti, L.; Russo, V.; Sarwar, S.; Schaerf, C.

1997-01-01

An experimental investigation has been carried out on the properties of electromagnetic shower detectors, composed of a uniform array of plastic scintillating fibers and lead (50:35 by volume ratio) for photons in the energy range 600-1200 MeV. When the photon's incidence angle to the fiber axis is within ±2 circle an energy resolution of σ E /E(%)=5.12/√(E[GeV])+1.71 has been observed. (orig.)

Analysis of the polymerization initiation and activity of Pasteurella multocida heparosan synthase PmHS2, an enzyme with glycosyltransferase and UDP-sugar hydrolase activity

NARCIS (Netherlands)

Chavaroche, A.A.E.; Broek, van den L.A.M.; Springer, J.; Boeriu, C.; Eggink, G.

2011-01-01

Heparosan synthase catalyzes the polymerization of heparosan [-4GlcUAß1-4GlcNAca1-]n by transferring alternatively the monosaccharide units from UDP-GlcUA and UDP-GlcNAc to an acceptor molecule. Details on the heparosan chain initiation by Pasteurella multocida heparosan synthase PmHS2 and its

Caracterización de los compuestos del aroma en rones colombianos por HS-SPME-GC-MS-O

Directory of Open Access Journals (Sweden)

Jazmín Osorio Monsalve

2016-11-01

Full Text Available Se identificaron por primera vez 46 compuestos volátiles olfativamente activos con actividad aromática significativa presentes en rones comerciales de Colombia. Lo anterior, mediante microextracción en fase sólida en modo espacio de cabeza (HS-SPME, acoplada a cromatografía de gases con detectores de espectrometría de masas y olfatometría (GC-MS-O. Dentro de los compuestos identificados se encuentran ésteres, compuestos aromáticos, alcoholes, acetales, lactonas y furfural. De esos 46 compuestos, nueve están presentes en todos los rones estudiados y algunos otros son característicos solo de la marca.

Functional homology of gHs and gLs from EBV-related γ-herpesviruses for EBV-induced membrane fusion

International Nuclear Information System (INIS)

Omerovic, Jasmina; Longnecker, Richard

2007-01-01

Epstein-Barr virus (EBV) is a human γ-herpesvirus that primarily infects B lymphocytes and epithelial cells. Entry of EBV into B cells requires the viral glycoproteins gp42, gH/gL and gB, while gp42 is not necessary for infection of epithelial cells. In EBV, gH and gL form two distinct complexes, a bipartite complex that contains only gH and gL, used for infection of epithelial cells, and a tripartite complex that additionally includes gp42, used for infection of B cells. The gH/gL complex is conserved within the herpesvirus family, but its exact role in entry and mechanism of fusion is not yet known. To understand more about the functionality of EBVgH/gL, we investigated the functional homology of gHs and gLs from human herpesvirus 8 (HHV8) and two primate (rhesus and marmoset) γ-herpesviruses in EBV-mediated virus-free cell fusion assay. Overall, gHs and gLs from the more homologous primate herpesviruses were better at complementing EBV gH and gL in fusion than HHV8 gH and gL. Interestingly, marmoset gH was able to complement fusion with epithelial cells, but not B cells. Further investigation of this led to the discovery that EBVgH is the binding partner of gp42 in the tripartite complex and the absence of fusion with B cells in the presence of marmoset gH/gL is due to its inability to bind gp42

Intermittent fasting during Ramadan causes a transient increase in total, LDL, and HDL cholesterols and hs-CRP in ethnic obese adolescents.

Science.gov (United States)

Radhakishun, Nalini; Blokhuis, Charlotte; van Vliet, Mariska; von Rosenstiel, Ines; Weijer, Olivier; Heymans, Martijn; Beijnen, Jos; Brandjes, Dees; Diamant, Michaela

2014-08-01

The radical change of lifestyle during Ramadan fast has shown to affect cardiometabolic risk variables in adults. In youth, however, no studies are available. We aimed to evaluate the effect of Ramadan fast on Body Mass Index (BMI) and the cardiometabolic profile of obese adolescents. A prospective cohort study was conducted. We measured weight, height, body composition, blood pressure, heart rate, glucose, insulin, total cholesterol, low-density lipoprotein (LDL) cholesterol and high-density lipoprotein (HDL) cholesterol, triglycerides, and high sensitivity C-reactive protein (hs-CRP) levels before, during the last week of and at 6 weeks after Ramadan. Twenty-five obese adolescents were included. BMI and glucose metabolism did not change after Ramadan or at 6 week after cessation of Ramadan. At the end of Ramadan, a significant decrease in body fat percentage was observed, while significant increases in heart rate, total cholesterol, LDL cholesterol, HDL cholesterol, and hs-CRP were found (all P < 0.05). Six weeks after Ramadan, all parameters returned to baseline levels. In this sample of 25 ethnic obese adolescents transient cardiometabolic changes were observed during Ramadan fasting. Since most of these changes were reversible within 6 weeks, there seems no harm or benefit for obese adolescents to participate in Ramadan.

Performance evaluation of LUMIPULSE G1200 autoimmunoanalyzer for the detection of serum hepatitis B virus markers.

Science.gov (United States)

Choi, Seung Jun; Park, Yongjung; Lee, Eun Young; Kim, Sinyoung; Kim, Hyon-Suk

2013-05-01

We evaluated recently introduced automated immunoassay analyzer LUMIPULSE G1200 (Fujirebio, Inc., Tokyo, Japan) for detecting serologic hepatitis B virus (HBV) markers by comparison with the results by ARCHITECT i4000SR (Abbott, Abbott Park, IL). Precision performance was evaluated over 20 days. HBV surface antigen (HBsAg), HBV e antigen (HBeAg), antibodies to HBV core antigen (anti-HBc), antibodies to HBeAg (anti-HBe), and antibodies to HBsAg (anti-HBs) in a total of 1,000 serum samples were assessed by the two analyzers. Discrepant results were retested by COBAS e411 (Roche Diagnostics, Mannheim, Germany). LUMIPULSE showed excellent precision performance of total imprecision less than 3.5% coefficient of variation. The qualitative results between the two analyzers were agreed with each other in 92.0-99.8% of the specimens according to the different HBV markers. The degrees of reactions for HBeAg were moderately correlated between the two analyzers (r = 0.60), and those of other HBV markers were well correlated (r = 0.80 or greater). However, there were 183 discrepancies among 1,000 cases, and most of them showed degree of reaction around the cutoff values. LUMIPULSE G1200 showed well-concordant results with ARCITHECT for hepatitis B serologic tests. However, results near the cutoff values would need to be retested with other immunoassay or molecular methods, when the serological profiles of HBV markers are unusual or are not correlated to the clinical conditions of the patient, due to discrepancies between the immunoassay analyzers. © 2013 Wiley Periodicals, Inc.

State of the art and challenges in development of electrical contact materials in the light of the RoHS directive

Directory of Open Access Journals (Sweden)

Ćosović V.

2012-01-01

Full Text Available The article surveys current state of the art and challenges in the development of the electrical contact materials in the light of the EU Directive on Restriction of Hazardous Substances (RoHS. The focus was placed on widely used silver-cadmium alloys. According to this directive, as of July 1, 2006, use of six hazardous materials, including cadmium, are restricted for applicable electrical and electronic products intended for the EU market. In contrast, traditionally preferred material for production of electrical contacts is Ag-CdO, due to its outstanding functional properties. These conflicting interests result in present state where RoHS directive has not yet been implemented in its original form and has undergone numerous amendments and exceptions regarding the use of cadmium. Main reason for this seems to be the unrealistic time frame imposed by legislation. Although, significant effort has been put into research and development of alternative materials, there are still cases where adequate replacement materials had not been found. Therefore, importance of synchronicity between legislation and technological progress i.e. communication between legislative administration and industry and academia is brought to light as well as some common issues that may arise with an introduction of new replacement materials or product modification. [Projekat Ministarstva nauke Republike SRbije, br. OI 172037 and TR 34023

Parcels and Land Ownership, Parcels derived from legal descriptions and surveys. Tied to section monuments with coordinates derived from traditional survey and GPS., Published in 2013, 1:1200 (1in=100ft) scale, Portage County Government.

Data.gov (United States)

NSGIC Local Govt | GIS Inventory — Parcels and Land Ownership dataset current as of 2013. Parcels derived from legal descriptions and surveys. Tied to section monuments with coordinates derived from...

Influence of harvest maturity and fruit logistics on pineapple (Ananas comosus [L.] Merr.) volatiles assessed by headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC/MS).

Science.gov (United States)

Steingass, Christof B; Grauwet, Tara; Carle, Reinhold

2014-05-01

Profiling of volatiles from pineapple fruits was performed at four ripening stages using headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC/MS). In total, 142 volatiles were detected, of which 132 were identified. Multivariate data analysis was carried out to assess the effect of post-harvest storage on volatiles composition of green-ripe sea-freighted pineapple in comparison to air-freighted fruits harvested at full maturity. The latter fruits were characterised by volatiles described as potent odorants in pineapples, such as δ-octalactone, γ-lactones, 1-(E,Z)-3,5-undecatriene and 1,3,5,8-undecatetraene, as well as various methyl esters. In contrast, post-harvest storage of green-ripe sea-freighted fruits resulted in an increased formation of ethyl esters, acetates, acetoxy esters and alcohols, thus allowing the authentication of sea- and air-freighted pineapples, respectively. Particularly, compounds presumably derived from methyl-branched amino acid catabolism were identified in the fruits at later post-harvest stages. In addition, physicochemical traits were determined to characterise the fruit maturity stages. Copyright © 2013 Elsevier Ltd. All rights reserved.

Effects of the whole-body cryotherapy on NTproBNP, hsCRP and troponin I in athletes.

Science.gov (United States)

Banfi, Giuseppe; Melegati, Gianluca; Barassi, Alessandra; d'Eril, Gianlodovico Melzi

2009-11-01

Whole-body cryotherapy refers to brief exposure to very cold air for treating symptoms of various illnesses. In sports medicine, whole-body cryotherapy is administered to improve recovery from muscular trauma. As specific studies are lacking, we measured cardiac markers in 10 top-level rugby players of the Italian National team before and after a 1-week course of daily sessions of whole-body cryotherapy. All subjects continued with the same training workload as that of the previous weeks. N-terminal pro B-type natriuretic peptide (NTproBNP) levels increased but remained within the normal range, whilst troponin I (TnI) and high sensitivity C-reactive protein (hsCRP) were unchanged. Whole-body cryotherapy did not impair cardiac function in this sample of elite athletes.

Late Glacial temperature and precipitation changes in the lowland Neotropics by tandem measurement of δ 18O in biogenic carbonate and gypsum hydration water

Science.gov (United States)

Hodell, David A.; Turchyn, Alexandra V.; Wiseman, Camilla J.; Escobar, Jaime; Curtis, Jason H.; Brenner, Mark; Gilli, Adrian; Mueller, Andreas D.; Anselmetti, Flavio; Ariztegui, Daniel; Brown, Erik T.

2012-01-01

We applied a new method to reconstruct paleotemperature in the tropics during the last deglaciation by measuring oxygen isotopes of co-occurring gypsum hydration water and biogenic carbonate in sediment cores from two lakes on the Yucatan Peninsula. Oxygen and hydrogen isotope values of interstitial and gypsum hydration water indicate that the crystallization water preserves the isotopic signal of the lake water, and has not undergone post-depositional isotopic exchange with sediment pore water. The estimated lake water δ18O is combined with carbonate δ18O to calculate paleotemperature. Three paired measurements of 1200-yr-old gypsum and gastropod aragonite from Lake Chichancanab, Mexico, yielded a mean temperature of 26 °C (range 23-29.5 °C), which is consistent with the mean and range of mean annual temperatures (MAT) in the region today. Paired measurements of ostracods, gastropods, and gypsum hydration water samples were measured in cores from Lake Petén Itzá, Guatemala, spanning the Late Glacial and early Holocene period (18.5-10.4 ka). The lowest recorded temperatures occurred at the start of Heinrich Stadial (HS) 1 at 18.5 ka. Inferred temperatures from benthic ostracods ranged from 16 to 20 °C during HS 1, which is 6-10 °C cooler than MAT in the region today, whereas temperatures derived from shallow-water gastropods were generally warmer (20-25 °C), reflecting epilimnetic temperatures. The derived temperatures support previous findings of greater tropical cooling on land in Central America during the Late Glacial than indicated by nearby marine records. Temperature increased in two steps during the last deglaciation. The first occurred during the Bolling-Allerod (B-A; from 14.7 to 13 ka) when temperature rose to 20-24 °C towards the end of this period. The second step occurred at 10.4 ka near the beginning of the Holocene when ostracod-inferred temperature rose to 26 °C, reflecting modern hypolimnetic temperature set during winter, whereas

The Relationship of Fetuin-A, Adiponectin, Retinol Binding Protein-4 (RBP-4 and High Sensitivity C-Reactive Protein (hsCRP with Insulin Resistance (HOMA-IR in Obese Non Diabetic Men

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Imelda Novianti

2012-04-01

Full Text Available BACKGROUND: Central obesity is the accumulation of visceral (intra-abdominal fat and is strongly known to be associated with insulin resistance and type 2 diabetes mellitus (T2DM. Obesity can cause adipocyte hypertrophy that results in dysregulation of adipokine expression. The abnormal function of adipocytes may play an important role in the development of a chronic low-grade proinflammatory state associated with obesity. Adiponectin, retinol binding protein (RBP-4 and fetuin-A play a role in the pathophysiology of insulin resistance. Expression of fetuin-A is increased due to fat accumulation in the liver. Elevated concentration of fetuin-A in the circulation can impair insulin signaling in muscle and liver as well as suppress adiponectin secretion, although its molecular mechanism is still unclear. The aim of this study was to identify the relationship of fetuin-A, adiponectin, RBP-4 and hsCRP with insulin resistance in obese non diabetic men. METHODS: This was an observational study with a cross-sectional design. The study subjects were 64 men with non diabetic abdominal obesity, characterized by waist circumference of 98.47±5.88 cm and fasting blood glucose of 85.75±8.36 mg/dL. RESULTS: This study showed that fetuin-A was positively correlated with HOMA-IR in obese non diabetic men with insulin resistance (r=0.128; p=0.570, although not significant. Fetuin-A was found to be correlated with adiponectin, RBP-4 and hsCRP (r=0.150; p=0.233; r=0.050; p=0.711; r=-0.04; p=0.445, although not significant. CONCLUSIONS: The concentration of fetuin-A showed a tendency to be positively correlated with HOMA-IR and with RBP-4 in obese non diabetic men, although statistically not significant. The concentration of fetuin-A showed a tendency to be negatively correlated with adiponectin and hsCRP although statistically not significant. There was no interrelationship between fetuin-A, adiponectin, RBP-4, hsCRP and HOMA-IR. Elevated concentrations of fetuin

Volatile emerging contaminants in melon fruits, analysed by HS-SPME-GC-MS.

Science.gov (United States)

Cincotta, Fabrizio; Verzera, Antonella; Tripodi, Gianluca; Condurso, Concetta

2018-03-01

The aim of this research was to develop and validate a headspace-solid phase micro-extraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) method for the determination of volatile emerging contaminants in fruit. The method showed good precision (RSD ≤ 14%) and satisfactory recoveries (99.1-101.7%) and LOD and LOQ values ranging between 0.011-0.033 μg kg -1 and 0.037-0.098 μg kg -1 , respectively. The method was applied to investigate the content of volatile emerging contaminants in two varieties of melon fruit (Cucumis melo L.) cultivated adjoining high-risk areas. Glycol ethers, BHT, BHA and BTEX (benzene, toluene, ethylbenzene and xylene) were determined in melon fruit pulps for the first time, with different sensitivities depending on sample and variety. Although the amount of the volatile contaminants in the melon samples were in the order of µg kg -1 , the safety of vegetable crops cultivated near risk areas should be more widely considered. The results showed that this accurate and reproducible method can be useful for routine safety control of fruits and vegetables.

Implementation of the resonant vibratory feeders control algorithm on Simatic S7-1200 from MATLAB Simulink enviroment

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Mitrović Radomir B.

2016-01-01

Full Text Available Simulink is an important tool for modeling and simulation of process and control algorithms. It's expansion, PLC Coder, enables direct conversion of model subsystem into SCL, structured text code, which is then used by PLC IDE to create function blocks. This shortens developing time of algorithms for PLC controller. Also, this reduces possibility for a coding error. This paper describes Simulink PLC Coder and workflow for developing PID control algorithm for Siemens Simatic S7-1200 PLC. Control object used here is resonant vibratory feeder having electromagnetic drive.

Evaluation of plasma concentrations of homocysteine, IL-6, TNF-alpha, hs-CRP, and total antioxidant capacity in patients with end-stage renal failure.

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Mahin Babaei

2014-12-01

Full Text Available It has been proved that hyperhomocysteinemia has a high prevalence in patients with end-stage renal disease (ESRD, which may contribute to the high cardiovascular risk in these patients. Cardiovascular disease is the first cause of high mortality rate in ESRD patients. The aim of the present study was to assess five important factors in patients with ESRD (the amount of homocysteine, IL-6, TNF-alpha, hs-CRP, and Total Antioxidant Capacity. These factors were surveyed in ESRD patients to compare with healthy subjects. In a cross-sectional study, we enrolled 80 patients on maintenance hemodialysis and measured the inflammatory and oxidative stress indicators. The plasma samples were assayed for five above mentioned variables using standard protocols. Two-hour post hemodialysis plasma samples were also assayed for TAC. Plasma levels of inflammation markers, IL-6 and hs-CRP, homocysteine were significantly increased in ESRD group versus control group. This increase was also found in TNF-α levels as compared to the controls, but the differences were not statistically significant. Also, the post dialysis samples had significantly lower levels of TAC as compared to predialysis ones.

The lands west of the lakes; A history of the Ajattappareng kingdoms of South Sulawesi 1200 to 1600 CE

OpenAIRE

Druce, Stephen C.

2009-01-01

The period 1200-1600 CE saw a radical transformation from simple chiefdoms to kingdoms (in archaeological terminology, complex chiefdoms) across lowland South Sulawesi, a region that lay outside the ‘classical’ Indicized parts of Southeast Asia. The rise of these kingdoms was stimulated and economically supported by trade in prestige goods with other parts of island Southeast Asia, yet the development of these kingdoms was determined by indigenous, rather than imported, political and cultural...

Influence of pH on pesticide sorption by soil containing wheat residue-derived char

International Nuclear Information System (INIS)

Sheng Guangyao; Yang Yaning; Huang Minsheng; Yang Kai

2005-01-01

Field burning of crop residues incorporates resulting chars into soil and may thus influence the environmental fate of pesticides in the soil. This study evaluated the influence of pH on the sorption of diuron, bromoxynil, and ametryne by a soil in the presence and absence of a wheat residue-derived char. The sorption was measured at pHs ∼3.0 and ∼7.0. Wheat char was found to be a highly effective sorbent for the pesticides, and its presence (1% by weight) in soil contributed >70% to the pesticide sorption (with one exception). The sorption of diuron was not influenced by pH, due to its electroneutrality. Bromoxynil becomes dissociated at high pHs to form anionic species. Its sorption by soil and wheat char was lower at pH ∼7.0 than at pH ∼3.0, probably due to reduced partition of the anionic species of bromoxynil into soil organic matter and its weak interaction with the carbon surface of the char. Ametryne in its molecular form at pH ∼7.0 was sorbed by char-amended soil via partitioning into soil organic matter and interaction with the carbon surface of the char. Protonated ametryne at pH ∼3.0 was substantially sorbed by soil primarily via electrostatic forces. Sorption of protonated ametryne by wheat char was also significant, likely due not only to the interaction with the carbon surface but also to interactions with hydrated silica and surface functional groups of the char. Sorption of ametryne by char-amended soil at pH ∼3.0 was thus influenced by both the soil and the char. Environmental conditions may thus significantly influence the sorption and behavior of pesticides in agricultural soils containing crop residue-derived chars. - Wheat char was effective for adsorption of pesticides in soil, with efficacy varying with pH and particular pesticides

Influence of pH on pesticide sorption by soil containing wheat residue-derived char

Energy Technology Data Exchange (ETDEWEB)

Sheng Guangyao [Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701 (United States)]. E-mail: gsheng@uark.edu; Yang Yaning [Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701 (United States); Huang Minsheng [Department of Environmental Science and Technology, East China Normal University, Shanghai 200062 (China); Yang Kai [Department of Environmental Science and Technology, East China Normal University, Shanghai 200062 (China)

2005-04-01

Field burning of crop residues incorporates resulting chars into soil and may thus influence the environmental fate of pesticides in the soil. This study evaluated the influence of pH on the sorption of diuron, bromoxynil, and ametryne by a soil in the presence and absence of a wheat residue-derived char. The sorption was measured at pHs {approx}3.0 and {approx}7.0. Wheat char was found to be a highly effective sorbent for the pesticides, and its presence (1% by weight) in soil contributed >70% to the pesticide sorption (with one exception). The sorption of diuron was not influenced by pH, due to its electroneutrality. Bromoxynil becomes dissociated at high pHs to form anionic species. Its sorption by soil and wheat char was lower at pH {approx}7.0 than at pH {approx}3.0, probably due to reduced partition of the anionic species of bromoxynil into soil organic matter and its weak interaction with the carbon surface of the char. Ametryne in its molecular form at pH {approx}7.0 was sorbed by char-amended soil via partitioning into soil organic matter and interaction with the carbon surface of the char. Protonated ametryne at pH {approx}3.0 was substantially sorbed by soil primarily via electrostatic forces. Sorption of protonated ametryne by wheat char was also significant, likely due not only to the interaction with the carbon surface but also to interactions with hydrated silica and surface functional groups of the char. Sorption of ametryne by char-amended soil at pH {approx}3.0 was thus influenced by both the soil and the char. Environmental conditions may thus significantly influence the sorption and behavior of pesticides in agricultural soils containing crop residue-derived chars. - Wheat char was effective for adsorption of pesticides in soil, with efficacy varying with pH and particular pesticides.

Effect of rosuvastatin dose-loading on serum sLox-1, hs-CRP, and postoperative prognosis in diabetic patients with acute coronary syndromes undergoing selected percutaneous coronary intervention (PCI).

Science.gov (United States)

Jiao, Yungen; Hu, Feng; Zhang, Zhengang; Gong, Kaizheng; Sun, Xiaoning; Li, Aihua; Liu, Naifeng

2015-01-01

To investigate the effect of rosuvastatin dose-loading on serum levels of lectin-like oxidized low-density lipoprotein receptor-1 (Lox-1) and high-sensitivity c-reactive protein (hs-CRP) and postoperative prognosis in patients with diabetes and non-ST segment elevation acute coronary syndromes (NSTEACS) undergoing selected percutaneous coronary intervention (PCI). A total of 72 patients with diabetes and NSTEACS were randomized to either the group treated with 20 mg rosuvastatin 12 hours prior to PCI with a second dose administered just before PCI (n = 33), or a control group treated with standard method according guideline (n = 39). Serum levels of sLox-1, hs-CRP, CK-MB, and cTnI were measured prior to PCI, and at 24 hours and 30 days after PCI. The 30-day incidence of major adverse cardiac events (MACE) was recorded in both groups. Compared to pre-PCI, serum levels of sLox-1 and hs-CRP of the two groups were increased at 24 hours after PCI (P PCI (P PCI, the levels of TC and LDL-C were not changed at 24 hours after PCI (P > 0.05) until 30 days after PCI (P PCI; the 30-day incidence of MACE occurred in 6.06% of patients in the loading-dose rosuvastatin-treated group and in 23.08% of patients in the control-treated group (P PCI, and also reduce the occurrence of MACE 30 days after PCI.

Restrição ao uso de substâncias perigosas (RoHS) no segmento de computadores pessoais: análise da estratégia de adoção pelos fabricantes estabelecidos no Brasil

OpenAIRE

Brescansin, Ademir

2014-01-01

A publicação, em 2003, da diretiva europeia limitando o uso das substâncias perigosas (chumbo, mercúrio, cádmio, cromo hexavalente e retardantes de chama à base de bromo) RoHS (Restriction of Hazardous Substances) obrigou os fabricantes a adequarem seus produtos e processos para atendimento à legislação. Muitas empresas fabricantes de equipamentos eletroeletrônicos, a partir de então, passaram a adotar os requisitos da RoHS para todos os seus produtos. No Brasil mesmo não existindo uma legisl...

A comparison of sample preparation methods for extracting volatile organic compounds (VOCs) from equine faeces using HS-SPME.

Science.gov (United States)

Hough, Rachael; Archer, Debra; Probert, Christopher

2018-01-01

Disturbance to the hindgut microbiota can be detrimental to equine health. Metabolomics provides a robust approach to studying the functional aspect of hindgut microorganisms. Sample preparation is an important step towards achieving optimal results in the later stages of analysis. The preparation of samples is unique depending on the technique employed and the sample matrix to be analysed. Gas chromatography mass spectrometry (GCMS) is one of the most widely used platforms for the study of metabolomics and until now an optimised method has not been developed for equine faeces. To compare a sample preparation method for extracting volatile organic compounds (VOCs) from equine faeces. Volatile organic compounds were determined by headspace solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GCMS). Factors investigated were the mass of equine faeces, type of SPME fibre coating, vial volume and storage conditions. The resultant method was unique to those developed for other species. Aliquots of 1000 or 2000 mg in 10 ml or 20 ml SPME headspace were optimal. From those tested, the extraction of VOCs should ideally be performed using a divinylbenzene-carboxen-polydimethysiloxane (DVB-CAR-PDMS) SPME fibre. Storage of faeces for up to 12 months at - 80 °C shared a greater percentage of VOCs with a fresh sample than the equivalent stored at - 20 °C. An optimised method for extracting VOCs from equine faeces using HS-SPME-GCMS has been developed and will act as a standard to enable comparisons between studies. This work has also highlighted storage conditions as an important factor to consider in experimental design for faecal metabolomics studies.

Restrained shrinkage experiments on coated particle fuel compacts in the temperature range 600-1200 deg C

International Nuclear Information System (INIS)

Blackstone, R.; Veringa, H.J.; Loelgen, R.

1976-05-01

Information on irradiation induced creep in reactor graphite and in fuel compact material is an essential ingredient in the design of any reactor core layout, because the creep plasticity of these materials diminishes the stresses which are built up in the fuel element during reactor operation. The restrained shrinkage method in which the shrinkage of a dumbbell shaped creep specimen is restrained by a graphite material which shows less irradiation shrinkage, offers a good possibility of performing a large series of tensile creep experiments in a limited irradiation volume. The irradiations, evaluations and the results of a series of restrained shrinkage experiments in which six different materials were tested, of which five were dummy coated particle compacts and one pure matrix material are described and discussed. These materials were irradiated in the High Flux Reactor of the Euratom Joint Research Centre in Petten/Netherlands. The irradiations were performed in three successive capsules at irradiation temperatures of 600 deg C, 900 deg C, 1050 deg C and 1200 deg C up to a neutron fluence of maximum 3x10 21 n.cm 2 (DNE). The post-irradiation examinations yielded plastic strains up to 2,3%, and values for the radiation creep coefficient were calculated, ranging from 4 to 8.10 -12 at 600 deg C and 8 to 30.10 -12 at 1200 deg C always given per dyn.cm -2 tensile stresses and per 10 20 n.cm -2 fluence units. Generally it was found that the creep behavior of these materials and the temperature dependence of the creep process could be compared with those for normal reactor graphites

Combining Magnetic Resonance Imaging (MRI) and Medical Infrared Thermography (MIT) in the pre- and per-operating management of severe Hidradenitis Suppurativa (HS).

Science.gov (United States)

Derruau, Stéphane; Renard, Yohann; Pron, Hervé; Taiar, Redha; Abdi, Ellie; Polidori, Guillaume; Lorimier, Sandrine

2018-05-12

Hidradenitis suppurativa (HS) is a chronic, inflammatory, and recurrent skin disease. Surgical excision of wounds appears to be the only curative treatment for the prevention of recurrence of moderate to severe stages. Magnetic resonance imaging (MRI) is a standard reference examination for the detection of HS peri-anal inflammatory fistula. In this case study, the use of real-time medical infrared thermography, in combination with MRI as appropriate imaging, is proposed. The aim is to assist surgeons in the pre- and peri-surgical management of severe perianal hidradenitis suppurativa with the intent to ensure that all diseased lesions were removed during surgery and therefore to limit recurrence. The results show that medical infrared thermography (MIT), coupled with MRI, could be highly effective strategy to address thermally distinguished health tissues and inflammatory sites during excision, as characterised by differential increases in temperature. Medical infrared thermography could be used to check the total excision of inflammatory lesions as a noninvasive method that is not painful, not radiant, and is easily transportable during surgery. Ultimately, this method could be complementary with MRI in providing clinicians with objective data on the status of tissues below the perianal skin surface in the pre- and per-operating management of severe hidradenitis suppurativa. Copyright © 2018 Elsevier B.V. All rights reserved.

N-terminal pro-brain natriuretic peptide measurements in hemodynamically significant patent ductus arteriosus in preterm infants.

Science.gov (United States)

Alenazi, Shehab Ahmed

2016-01-01

Evaluate the role of NT-proBNP levels in Preterm neonates suffering from PDA and used as a screening tool for predicting HsPDA and guiding physicians to consider early echocardiographic evaluation. This is a monocentric prospective blind study which was conducted at Arar Central Hospital, Ar'ar, Saudi Arabia, during the period between Jan 2014 to June 2014. Thirty-three (33) preterm infants born at less than 31 weeks of gestation or weighing less than 1200 g at birth infants were initially enrolled during a 6-month period. Blood samples were collected along with routine blood tests on days 1, 2, 3, and 7 of life for NT-proBNP analysis. Two echocardiographies were systematically performed on day two of life to ascertain about the status of Ductus Arteriosus. The Plasma NT-proBNP levels were high on day one of life and decline from day three to day seven of life except in those infants with significant hsPDA. Plasma NT-proNBP levels on day 2 of infants in the HsPDA group were significantly higher (ventricular posterior wall thickness (pclosure or otherwise.

Oxidation of Zircaloy-4 in steam-nitrogen mixtures at 600-1200 °C

Science.gov (United States)

Steinbrueck, Martin; da Silva, Fabio Oliveira; Grosse, Mirco

2017-07-01

High-temperature oxidation of zirconium alloys in steam-nitrogen atmospheres may be relevant during various nuclear accident scenarios. Therefore, isothermal oxidation tests with Zircaloy-4 in steam-nitrogen mixtures have been performed at 600, 800, 1000, and 1200 °C using thermogravimetry. The gas compositions were varied between 0 and 100 vol% nitrogen including 0.1 and 90 vol%. The strong effect of nitrogen on the oxidation kinetics of zirconium alloys was confirmed in these tests in mixed steam-nitrogen atmospheres. Even very low concentrations of nitrogen (starting from less than 1 vol%) strongly increase reaction kinetics. Nitrogen reduces transition time from protective to non-protective oxide scale (breakaway). The formation of zirconium nitride, ZrN, and its re-oxidation is the main reason for the highly porous oxide scales after transition. The results of this study have shown the safety relevant role of nitrogen during severe accidents and, more generally, suggest the need of using well controlled gas atmospheres for experiments on oxidation of zirconium alloys.

Bile acid metabolism in cirrhosis. VIII. Quantitative evaluation of bile acid synthesis from [7 beta-3H]7 alpha-hydroxycholesterol and [G-3H]26-hydroxycholesterol

International Nuclear Information System (INIS)

Goldman, M.; Vlahcevic, Z.R.; Schwartz, C.C.; Gustafsson, J.; Swell, L.

1982-01-01

In order to evaluate more definitively the observed aberrations in the synthesis of cholic and chenodeoxycholic acids in patients with advanced cirrhosis, two bile acid biosynthesis pathways were examined by determining the efficiency of conversion of [ 3 H]7 alpha-hydroxycholesterol and [ 3 H] 26-hydroxycholesterol to primary bile acids. Bile acid kinetics were determined by administration of [ 14 C]cholic and [ 14 C]chenodeoxycholic acids. Cholic acid synthesis in cirrhotic patients was markedly depressed (170 vs 927 μmoles per day)( while chenodeoxycholic acid synthesis was reduced to a much lesser degree (227 vs 550 μmoles per day). The administration of [ 3 H]7 alpha-hydroxycholesterol allowed for an evaluation of the major pathway of bile acid synthesis via the 7 alpha-hydroxylation of cholesterol. This compound was efficiently incorporated into primary bile acids by the two normal subjects (88 and 100%) and two cirrhotic patients (77 and 91%). However, the recovery of the label in cholic acid was slightly less in cirrhotic patients than in normal subjects. [ 3 H]26-hydroxycholesterol was administered to ascertain the contribution of the 26-hydroxylation pathway to bile acid synthesis. All study subjects showed poor conversion (9 to 22%) of this intermediate into bile acids. The results of this study suggest that a major block in the bile acid synthesis pathway in cirrhosis is at the level of 7 alpha-hydroxylation of cholesterol (impairment of 7 alpha-hydroxylase) and/or in the feedback triggering mechanism regulating bile acid synthesis. The data also suggest that the 26-hydroxylation pathway in normal subjects and patients with cirrhosis is a minor contributor to synthesis of the primary bile acids. Therefore, the relative sparing of chenodeoxycholic acid synthesis observed in cirrhotic patients is not due to preferential synthesis of this bile acid via the 26-hydroxylation pathway

The influence of remelting parameters of the electric arc and conventional tempering on the tribological resistance of high speed steel HS 6-5-2

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A. Dziedzic

2011-07-01

Full Text Available The present thesis depicts the results of the research of tribological high speed steel HS 6-5-2 remelted with the electric arc. Steel was remelted with different parameters. The amperage of electric arc was changed, the scanning speed was changed and the single, overlapping remeltings were used. There was also the influence of conventional tempering defined, which was conducted after remelting on the tribological resistance of hardened steel. For the previously mentioned processing variants, the intensity of tribological wear was defined and the linear wear were presented, and the friction coefficients. The type of tribological wear was also given, present during the friction, technically dry, of the hardened steel. The lower intensity of tribological wear was received for the single remelting by electric arc of 50 and 70A. Using the overlapping remeltings for the strengening of the surface layer of the high speed steel HS 6-5-2 causes the increase of the intensity of tribological wear in comparison to the steel with the single remelting. The conventional tempering leads to the decrease of the intensity of tribological wear.

Effect of Human Serum and 2 Different Types of Platelet Concentrates on Human Meniscus Cell Migration, Proliferation, and Matrix Formation.

Science.gov (United States)

Freymann, Undine; Metzlaff, Sebastian; Krüger, Jan-Philipp; Hirsh, Glen; Endres, Michaela; Petersen, Wolf; Kaps, Christian

2016-06-01

To evaluate the effect of 10% human serum (HS), 5% platelet-rich plasma (PRP), and 5% autologous conditioned plasma (ACP) on migration, proliferation, and extracellular matrix (ECM) synthesis of human meniscus cells. Cell migration and proliferation on stimulation with HS, PRP, and ACP were assessed by chemotaxis assays and measurement of genomic DNA content. Meniscus cells were cultivated in pellets stimulated with 10% HS, 5% PRP, or 5% ACP. Meniscal ECM formation was evaluated by histochemical staining of collagen type I, type II, and proteoglycans and by analysis of fibrochondrocyte marker gene expression. Human meniscus cells were significantly attracted by all 3 blood-derived products (10% HS and 5% ACP: P = .0001, 5% PRP: P = .0002). Cell proliferation at day 9 was significantly increased on stimulation with 10% HS (P = .0001) and 5% PRP (P = .0002) compared with 5% ACP and controls. Meniscus cell pellet cultures showed the formation of a well-structured meniscal ECM with deposition of collagen type I, type II, and proteoglycans on stimulation with 10% HS, whereas 5% PRP or 5% ACP resulted in the formation of an inhomogeneous and more fibrous ECM. Stimulation with 10% HS and 5% ACP showed a significant induction of fibrochondrocyte marker genes such as aggrecan (HS: P = .0002, ACP: P = .0147), cartilage oligomeric matrix protein (HS: P = .0002, ACP: P = .0005), and biglycan (HS: P = .0002, ACP: P = .0003), whereas PRP showed no inducing effect. Among all tested blood-derived products, only stimulation with HS showed the formation of a meniscal ECM as well as positive cell proliferating and migrating effects in vitro. Regarding a potential biological repair of nonvascular meniscus lesions, our results may point toward the use of HS as a beneficial augment in regenerative meniscus repair approaches. Our findings may suggest that HS might be a beneficial augment for meniscus repair. Copyright © 2016 Arthroscopy Association of North America. Published

Comparison of Lumipulse® G1200 with Kryptor and Modular E170 for the measurement of 7 tumor markers

OpenAIRE

Marlet , Julien; Bernard , Maguy

2014-01-01

"This is the pre-peer reviewed version of the following article: "Comparison of LUMIPULSE® G1200 With Kryptor and Modular E170 for the Measurement of Seven Tumor Markers", which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jcla.21802/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."; Background: Tumor marker measurements are becoming essential for prognosis and follow-up of patien...

DESIGN AND EXPERIENCE WITH THE WS/HS ASSEMBLY MOVEMENT USING LABVIEW VIS, NATIONAL INSTRUMENT MOTION CONTROLLERS, AND COMPUMOTOR ELECTRONIC DRIVE UNITS AND MOTORS

International Nuclear Information System (INIS)

Barr, D.S.; Day, L.A.

2001-01-01

The Low-Energy Demonstration Accelerator (LEDA), designed and built at the Los Alamos National Laboratory, is part of the Accelerator Production of Tritium (APT) program and provides a platform for measuring high-power proton beam-halo formation. The technique used for measuring the beam halo employs nine combination Wire Scanner and Halo Scraper (WS/HS) devices. This paper will focus on the experience gained in the use of National Instrument (NI) LabVIEW VIs and motion controllers, and Compumotor electronic drive units and motors. The base configuration couples a Compumotor motor driven by a Parker-Hannifin Gemini GT Drive unit. The drive unit is controlled by a NI PXI-7344 controller card, which in turn is controlled by a PC running custom built NI LabVIEW VIs. The function of the control VI's is to interpret instructions from the main control system, the Experimental Physics and Industrial Control System (EPICS), and carry out the corresponding motion commands. The main control VI has to run all nineteen WS/HS motor axes used in the accelerator. A basic discussion of the main accelerator control system, EPICs which is hosted on a VXI platform, and its interface with the PC based LabVIEW motion control software will be included

Determination of infinite dilution activity coefficients using HS-SPME/GC/FID for hydrocarbons in furfural at temperatures of (298.15, 308.15, and 318.15) K

International Nuclear Information System (INIS)

Arantes Furtado, Filipe; Vieira Coelho, Gerson Luiz

2012-01-01

Highlights: ► Two approaches were proposed using SPME on determination of infinite dilution activity coefficients. ► Infinite dilution activity coefficients of nine solutes in solvent furfural at T = (298.15, 308.15, and 318.15) K. ► Fiber–gas partition coefficients of nine solutes on PDMS at T = (298.15, 308.15, and 318.15) K. ► Optical microscopy analysis and statistical tests to measure possible damages on fiber coating. ► Advantages and limitations of methodology proposed were discussed. - Abstract: A new methodology using the headspace solid phase microextraction (HS-SPME) technique has been used to evaluate the infinite dilution activity coefficient (γ 12 ∞ ) of nine hydrocarbons (alkanes, cycloalkanes, and aromatics) in furfural solvent. The main objective of this study was to validate a faster and lower cost methodology expanding the use of HS-SPME to determine infinite dilution activity of solutes in organic solvents. Two approaches were proposed for the determination of γ 12 ∞ in order to use this technique (HS-SPME). In addition, the fiber–gas partition coefficients (K fg ) for each analyte at each of the studied temperatures were determined. The activity and partition coefficients have been reported at temperatures of (298.15, 308.15, and 318.15) K. The data were compared with the literature infinite dilution data determined by other methods such as liquid–gas chromatography (GLC) and gas stripping. Partial molar excess enthalpies of mixing at infinite dilution for each solute have been determined. The fibers were tested before and after each experiment, using statistical methods to ensure that their properties do not change during the experiments. The fibers were also analyzed by optical microscopy to evaluate possible surface damage by comparing them with new fibers. The activity coefficient values correlated well with the data in the literature and showed average deviations less than 10%.

Simple method for the calculation and use of CVD phase diagrams with applications to the Ti-B-Cl-H system, 1200 to 8000K

International Nuclear Information System (INIS)

Randich, E.; Gerlach, T.M.

1980-03-01

A simple method for calculating multi-component gas-solid equilibrium phase diagrams for chemical vapor deposition (CVD) systems is presented. The method proceeds in three steps: dtermination of stable solid assemblages, evaluation of gas-solid stability relations, and calcuation of conventional phase diagrams using a new free energy minimization technique. The phase diagrams can be used to determine (1) bulk compositions and phase fields accessible by CVD techniques; (2) expected condensed phases for various starting gas mixtures; and (3) maximum equilibrium yields for specific CVD process variables. The three step thermodynamic method is used to calcuate phase diagrams for the example CVD system Ti-B-Cl-H at 1200 and 800 0 K. Examples of applications of the diagrams for yield optimization and experimental accessibility studies are presented and discussed. Experimental verification of the TiB 2 + Gas/Gas phase field boundary at 1200 0 K, H/Cl = 1 confirms the calculated boundary and indicates that equilibrium is nearly and rapidly approached under laboratory conditions

An Innovative Rapid Method for Analysis of 10 Organophosphorus Pesticide Residues in Wheat by HS-SPME-GC-FPD/MSD.

Science.gov (United States)

Du, Xin; Ren, YongLin; Beckett, Stephen J

2016-01-01

The rapid detection of pesticide residues in wheat has become a top food security priority. A solvent-free headspace solid-phase microextraction (HS-SPME) has been evaluated for rapid screening of organophosphorus pesticide (OPP) residues in wheat with high sensitivity. Individual wheat samples (1.7 g), spiked with 10 OPPs, were placed in a 4 mL sealed amber glass vial and heated at 60°C for 45 min. During this time, the OPP residues were extracted with a 50 μm/30 μm divinylbenzene (DVB)/carboxen (CAR)/plasma desorption mass spectroscopy polydimethylsiloxane (PDMS) fiber from the headspace above the sample. The fiber was then removed and injected into the GC injection port at 250°C for desorption of the extracted chemicals. The multiple residues were identified by a GC mass spectrometer detector (GC-MSD) and quantified with a GC flame photometric detector (GC-FPD). Seven spiked levels of 10 OPPs on wheat were analyzed. The GC responses for a 50 μm/30 μm DVB/CAR/PDMS fiber increased with increasing spiking levels, yielding significant (R(2) > 0.98) linear regressions. The lowest LODs of the multiple pesticide standards were evaluated under the conditions of the validation study in a range of levels from 0 (control) to 100 ng of pesticide residue per g of wheat that separated on a low-polar GC capillary column (Agilent DB-35UI). The results of the HS-SPME method were compared with the QuEChERS AOAC 2007.01 method and they showed several advantages over the latter. These included improved sensitivity, selectivity, and simplicity.

Canthaxanthin biosynthesis by Dietzia natronolimnaea HS-1: effects of inoculation and aeration rate

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Forouzan Rostami

2014-06-01

Full Text Available The interest in production of natural colorants by microbial fermentation has been currently increased. The effects of D-glucose concentration (3.18-36.82 g/L, inoculum size (12.5 x 10(9-49.5 x 10(9 cfu cells/mL and air-flow rate (1.95-12.05 L/L min on the biomass, total carotenoid and canthaxanthin (CTX accumulation of Dietzia natronolimnaea HS-1 in a batch bioreactor was scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD. Second-order polynomial models with high R² values ranging from 0.978 to 0.990 were developed for the studied responses using multiple linear regression analysis. The models showed the maximum cumulative amounts of biomass (7.85 g/L, total carotenoid (5.48 mg/L and CTX (4.99 mg/L could be achieved at 23.38 g/L of D-glucose, 31.2 x 10(9 cfu cells/mL of inoculation intensity and air-flow rate of 7.85 L/L min. The predicted values for optimum conditions were in good agreement with experimental data.

Tailoring acyclovir prodrugs with enhanced antiviral activity: rational design, synthesis, human plasma stability and in vitro evaluation.

Science.gov (United States)

Chayrov, Radoslav L; Stylos, Evgenios K; Chatziathanasiadou, Maria V; Chuchkov, Kiril N; Tencheva, Aleksandra I; Kostagianni, Androniki D; Milkova, Tsenka S; Angelova, Assia L; Galabov, Angel S; Shishkov, Stoyan A; Todorov, Daniel G; Tzakos, Andreas G; Stankova, Ivanka G

2018-05-19

Bile acid prodrugs have served as a viable strategy for refining the pharmaceutical profile of parent drugs through utilizing bile acid transporters. A series of three ester prodrugs of the antiherpetic drug acyclovir (ACV) with the bile acids cholic, chenodeoxycholic and deoxycholic were synthesized and evaluated along with valacyclovir for their in vitro antiviral activity against herpes simplex viruses type 1 and type 2 (HSV-1, HSV-2). The in vitro antiviral activity of the three bile acid prodrugs was also evaluated against Epstein-Barr virus (EBV). Plasma stability assays, utilizing ultra-high performance liquid chromatography coupled with tandem mass spectrometry, in vitro cytotoxicity and inhibitory experiments were conducted in order to establish the biological profile of ACV prodrugs. The antiviral assays demonstrated that ACV-cholate had slightly better antiviral activity than ACV against HSV-1, while it presented an eight-fold higher activity with respect to ACV against HSV-2. ACV-chenodeoxycholate presented a six-fold higher antiviral activity against HSV-2 with respect to ACV. Concerning EBV, the highest antiviral effect was demonstrated by ACV-chenodeoxycholate. Human plasma stability assays revealed that ACV-deoxycholate was more stable than the other two prodrugs. These results suggest that decorating the core structure of ACV with bile acids could deliver prodrugs with amplified antiviral activity.

An improved method to prepare an injectable microemulsion of the galanin-receptor 3 selective antagonist, SNAP 37889, using Kolliphor® HS 15

Science.gov (United States)

Scheller, Karlene J.; Williams, Spencer J.; Lawrence, Andrew J.; Jarrott, Bevyn; Djouma, Elvan

2014-01-01

Research into the galanin-3 (GAL3) receptor has many challenges, including the lack of commercially available selective ligands. While the identification of non-peptidergic GAL3 receptor-selective antagonists, 1-phenyl-3-[3-(trifluoromethyl)phenyl]iminoindol-2-one (SNAP 37889) and 1-[3-(2-pyrrolidin-1-ylethoxy)phenyl]-3-[3-(trifluoromethyl)phenyl]iminoindol-2-one (SNAP 398299) have implicated a role for GAL3 receptors in anxiety, depression and drug-seeking behaviour, a major limitation of their use is poor aqueous solubility. Previously we have used 5% dimethylsulfoxide (DMSO) with 1% hydroxypropylmethyl cellulose in saline to dissolve SNAP 37889 for intraperitoneal (i.p.) injections of rats; however this produced a micro-suspension that was not ideal. The injectable formulation of SNAP 37889 was improved as follows:•30% (w/v) Kolliphor® HS 15 (Solutol HS® 15) and sodium phosphate buffer (0.01 M, pH 7.4) were used as vehicles.•A smooth glass mortar and pestle was used to triturate the Kolliphor® HS 15 and SNAP 37889 into a paste before addition to the sodium phosphate buffer at room temperature (RT).•The resulting mixture was vortexed until the paste was fully dissolved and the microemulsion was allowed to sit for 20 min to allow air bubbles to coalesce. PMID:26150955

A Modified Constitutive Model for Tensile Flow Behaviors of BR1500HS Ultra-High-Strength Steel at Medium and Low Temperature Regions

Science.gov (United States)

Zhao, Jun; Quan, Guo-Zheng; Pan, Jia; Wang, Xuan; Wu, Dong-Sen; Xia, Yu-Feng

2018-01-01

Constitutive model of materials is one of the most requisite mathematical model in the finite element analysis, which describes the relationships of flow behaviors with strain, strain rate and temperature. In order to construct such constitutive relationships of ultra-high-strength BR1500HS steel at medium and low temperature regions, the true stress-strain data over a wide temperature range of 293-873 K and strain rate range of 0.01-10 s-1 were collected from a series of isothermal uniaxial tensile tests. The experimental results show that stress-strain relationships are highly non-linear and susceptible to three parameters involving temperature, strain and strain rate. By considering the impacts of strain rate and temperature on strain hardening, a modified constitutive model based on Johnson-Cook model was proposed to characterize flow behaviors in medium and low temperature ranges. The predictability of the improved model was also evaluated by the relative error (W(%)), correlation coefficient (R) and average absolute relative error (AARE). The R-value and AARE-value for modified constitutive model at medium and low temperature regions are 0.9915 & 1.56 % and 0.9570 & 5.39 %, respectively, which indicates that the modified constitutive model can precisely estimate the flow behaviors for BR1500HS steel in the medium and low temperature regions.

Preparation and evaluation of icariside II-loaded binary mixed micelles using Solutol HS15 and Pluronic F127 as carriers.

Science.gov (United States)

Hou, Jian; Wang, Jing; Sun, E; Yang, Lei; Yan, Hong-Mei; Jia, Xiao-Bin; Zhang, Zhen-Hai

2016-11-01

An effective anti-cancer drug, icariside II (IS), has been used to treat a variety of cancers in vitro. However, its poor aqueous solubility and permeability lead to low oral bioavailability. The aim of this work was to use Solutol®HS15 and Pluronic F127 as surfactants to develop novel mixed micelles to enhance the oral bioavailability of IS by improving permeability and inhibiting efflux. The IS-loaded mixed micelles were prepared using the method of ethanol thin-film hydration. The physicochemical properties, dissolution property, oral bioavailability of the male SD rats, permeability and efflux of Caco-2 transport models, and gastrointestinal safety of the mixed micelles were evaluated. The optimized IS-loaded mixed micelles showed that at 4:1 ratio of Solutol®HS15 and Pluronic F127, the particle size was 12.88 nm with an acceptable polydispersity index of 0.172. Entrapment efficiency (94.6%) and drug loading (9.7%) contributed to the high solubility (11.7 mg/mL in water) of IS, which increased about 900-fold. The SF-IS mixed micelle release profile showed a better sustained release property than that of IS. In Caco-2 cell monolayer models, the efflux ratio dramatically decreased by 83.5%, and the relative bioavailability of the mixed micelles (AUC 0-∞ ) compared with that of IS (AUC 0-∞ ) was 317%, indicating potential for clinical application. In addition, a gastrointestinal safety assay also provided reliable clinical evidence for the safe use of this micelle.

Optimization of an analytical methodology for the determination of alkyl- and methoxy-phenolic compounds by HS-SPME in biomass smoke

Energy Technology Data Exchange (ETDEWEB)

Conde, Francisco J.; Afonso, Ana M.; Gonzalez, Venerando; Ayala, Juan H. [University of La Laguna, Campus de Anchieta, Department of Analytical Chemistry, Nutrition and Food Science, La Laguna (Spain)

2006-08-15

A sampling and analysis method for the determination of 21 phenolic compounds in smoke samples from biomass combustion has been developed. The smoke is used to make smoked foods, following an artisanal procedure used in some parts of the Canary Islands. The sampling system consists of a Bravo H air sampler, two impingers, each one containing an aqueous solution of sodium hydroxide 0.1 mol L{sup -1}, followed by a silica gel trap. The variables optimized to reach the best sampling conditions were volume of absorbent solution and sampling flow. Under the optimum conditions, 100 mL of absorbent solution of NaOH 0.10 mol L{sup -1} and 2 L min{sup -1} for the sampling flow, sampling efficiencies are higher than 80%. Analysis of phenolic compounds was carried out by headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS). Five different fiber coatings were employed in this study. By means of a central composite design, extraction time, salt concentration, and pH of the solution were optimized: 65-{mu}m carbowax-divinylbenzene, extraction time 90 min, concentration in NaCl of 35% (m/v), and pH 2 yielded the highest response. Detection limits of phenol and their alkyl derivatives, guaiacol and eugenol, are between 1.13 and 4.60 ng mL{sup -1}. 3-Methoxyphenol, 2,6-dimethoxyphenol, and vanillin have detection limits considerably higher. Good linearity (R {sup 2}{>=}0.98) was observed for all calibration curves in the established ranges. The reproducibility of the method (RSD, relative standard deviation) was found to oscillate between 7 and 18% (generally close or lower than 10%). (orig.)

On Hubbard-Stratonovich transformations over hyperbolic domains

International Nuclear Information System (INIS)

Fyodorov, Yan V

2005-01-01

We discuss and prove the validity of the Hubbard-Stratonovich (HS) identities over hyperbolic domains which are used frequently in studies on disordered systems and random matrices. We also introduce a counterpart of the HS identity arising in disordered systems with 'chiral' symmetry. Apart from this we outline a way of deriving the nonlinear σ-model from the gauge-invariant Wegner k-orbital model avoiding the use of the HS transformations

Equivalence of a single dose (1200 mg) compared to a three-time a day dose (400 mg) of chondroitin 4&6 sulfate in patients with knee osteoarthritis. Results of a randomized double blind placebo controlled study.

Science.gov (United States)

Zegels, B; Crozes, P; Uebelhart, D; Bruyère, O; Reginster, J Y

2013-01-01

Evaluation of the efficacy and safety of a single oral dose of a 1200 mg sachet of chondroitin 4&6 sulfate (CS 1200) vs three daily capsules of chondroitin 4&6 sulfate 400 mg (CS 3*400) (equivalence study) and vs placebo (superiority study) during 3 months, in patients with knee osteoarthritis (OA). Comparative, double-blind, randomized, multicenter study, including 353 patients of both genders over 45 years with knee OA. Minimum inclusion criteria were a Lequesne index (LI) ≥ 7 and pain ≥ 40 mm on a visual analogue scale (VAS). LI and VAS were assessed at baseline and after 1-3 months. Equivalence between CS was tested using the per-protocol procedure and superiority of CS vs placebo was tested using an intent-to-treat procedure. After 3 months of follow-up, no significant difference was demonstrated between the oral daily single dose of CS 1200 formulation and the three daily capsules of CS 400. Patients treated with CS 1200 or CS 3*400 were significantly improved compared to placebo after 3 months of follow-up in terms of LI (security and tolerability was observed between the three groups. This study suggests that a daily administration of an oral sachet of 1200 mg of chondroitin 4&6 sulfate allows a significant clinical improvement compared to a placebo, and a similar improvement when compared to a regimen of three daily capsules of 400 mg of the same active ingredient. Copyright © 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

HS-SPME GC/MS characterization of volatiles in raw and dry-roasted almonds (Prunus dulcis).

Science.gov (United States)

Xiao, Lu; Lee, Jihyun; Zhang, Gong; Ebeler, Susan E; Wickramasinghe, Niramani; Seiber, James; Mitchell, Alyson E

2014-05-15

A robust HS-SPME and GC/MS method was developed for analyzing the composition of volatiles in raw and dry-roasted almonds. Almonds were analyzed directly as ground almonds extracted at room temperature. In total, 58 volatiles were identified in raw and roasted almonds. Straight chain aldehydes and alcohols demonstrated significant but minimal increases, while the levels of branch-chain aldehydes, alcohols, heterocyclic and sulfur containing compounds increased significantly (500-fold) in response to roasting (palmonds) to 315.8±70.0 ng/g (averaged across the roasting treatments evaluated i.e. 28, 33 and 38 min at 138 °C) after roasting. Pyrazines were detected in only the roasted almonds, with the exception of 2,5-dimethylpyrazine, which was also found in raw almonds. The concentration of most alcohols increased in the roasted samples with the exception of 2-methyl-1-propanol, 3-methyl-1-butanol and 2-phenylethyl alcohol, which decreased 68%, 80%, and 86%, respectively. Copyright © 2013 Elsevier Ltd. All rights reserved.

High-sensitivity modified Glasgow prognostic score (HS-mGPS) Is superior to the mGPS in esophageal cancer patients treated with chemoradiotherapy

OpenAIRE

Chen, Peng; Fang, Min; Wan, Qiuyan; Zhang, Xuebang; Song, Tao; Wu, Shixiu

2017-01-01

The present study compared the prognostic value of the modified Glasgow prognostic score (mGPS) and high-sensitivity mGPS (HS-mGPS) in unresectable locally advanced esophageal squamous cell carcimona (LAESCC) patients treated with concurrent chemoradiotherapy (CCRT). The baseline data of 163 eligible patients were retrospectively collected. Patients with a C-reactive protein (CRP) ≤ 10 mg/l and albumin ≥ 35 g/l were allocated to mGPS-0 group. Patients with only elevated CRP (> 10 mg/l) were a...

Factor H and Properdin Recognize Different Epitopes on Renal Tubular Epithelial Heparan Sulfate

NARCIS (Netherlands)

Zaferani, Azadeh; Vives, Romain R.; van der Pol, Pieter; Navis, Gerjan J.; Daha, Mohamed R.; van Kooten, Cees; Lortat-Jacob, Hugues; Seelen, Marc A.; van den Born, Jacob

2012-01-01

During proteinuria, renal tubular epithelial cells become exposed to ultrafiltrate-derived serum proteins, including complement factors. Recently, we showed that properdin binds to tubular heparan sulfates (HS). We now document that factor H also binds to tubular HS, although to a different epitope

El Psicodiagnóstico de Rorschach como instrumento evaluador de grupos: un estudio de 1,200 casos

Directory of Open Access Journals (Sweden)

Matilde Ráez de Ramírez

1986-06-01

Full Text Available El presente trabajo intenta demostrar la utilidad del Psicodiagnóstico de Rorschach como instrumento que permite evaluar cuantitativamente y cualitativamente aspectos internos de la personalidad de grupos específicos. Para tal efecto se administró la prueba colectivamente a 1,200 sujetos de ambos sexos entre los 19 y 38 años que se encontraban postulando a un instituto superior o una promoción laboral. El análisis de los contenidos permite ubicar semejanzas y diferencias entre los grupos dentro de la normalidad, de acuerdo al sexo, edad y grado de instrucción, siendo este último el que parece identificar mayores diferencias.

FINAL REPORT START-UP AND COMMISSIONING TESTS ON THE DURAMELTER 1200 HLW PILOT MELTER SYSTEM USING AZ-101 HLW SIMULANTS VSL-01R0100-2 REV 0 1/20/03

Energy Technology Data Exchange (ETDEWEB)

KRUGER AA; MATLACK KS; KOT WK; BRANDYS M; WILSON CN; SCHATZ TR; GONG W; PEGG IL

2011-12-29

This document provides the final report on data and results obtained from commissioning tests performed on the one-third scale DuraMelter{trademark} 1200 (DM 1200) HLW Pilot Melter system that has been installed at VSL with an integrated prototypical off-gas treatment system. That system has replaced the DM1000 system that was used for HLW throughput testing during Part BI [1]. Both melters have similar melt surface areas (1.2 m{sup 2}) but the DM1200 is prototypical of the present RPP-WTP HLW melter design whereas the DM1000 was not. These tests were performed under a corresponding RPP-WTP Test Specification and associated Test Plan. This report is a followup to the previously issued Preliminary Data Summary Report. The DM1200 system will be used for testing and confirmation of basic design, operability, flow sheet, and process control assumptions as well as for support of waste form qualification and permitting. This will include data on processing rates, off-gas treatment system performance, recycle stream compositions, as well as process operability and reliability. Consequently, this system is a key component of the overall HLW vitrification development strategy. The results presented in this report are from the initial series of short-duration tests that were conducted to support the start-up and commissioning of this system prior to conducting the main body of development tests that have been planned for this system. These tests were directed primarily at system 'debugging,' operator training, and procedure refinement. The AZ-101 waste simulant and glass composition that was used for previous testing was selected for these tests.

Final Report Start-Up And Commissioning Tests On The Duramelter 1200 HLW Pilot Melter System Using AZ-101 HLW Simulants VSL-01R0100-2, Rev. 0, 1/20/03

International Nuclear Information System (INIS)

Kruger, A.A.; Matlack, K.S.; Kot, W.K.; Brandys, M.; Wilson, C.N.; Schatz, T.R.; Gong, W.; Pegg, I.L.

2011-01-01

This document provides the final report on data and results obtained from commissioning tests performed on the one-third scale DuraMelter(trademark) 1200 (DM 1200) HLW Pilot Melter system that has been installed at VSL with an integrated prototypical off-gas treatment system. That system has replaced the DM1000 system that was used for HLW throughput testing during Part BI (1). Both melters have similar melt surface areas (1.2 m 2 ) but the DM1200 is prototypical of the present RPP-WTP HLW melter design whereas the DM1000 was not. These tests were performed under a corresponding RPP-WTP Test Specification and associated Test Plan. This report is a followup to the previously issued Preliminary Data Summary Report. The DM1200 system will be used for testing and confirmation of basic design, operability, flow sheet, and process control assumptions as well as for support of waste form qualification and permitting. This will include data on processing rates, off-gas treatment system performance, recycle stream compositions, as well as process operability and reliability. Consequently, this system is a key component of the overall HLW vitrification development strategy. The results presented in this report are from the initial series of short-duration tests that were conducted to support the start-up and commissioning of this system prior to conducting the main body of development tests that have been planned for this system. These tests were directed primarily at system 'debugging,' operator training, and procedure refinement. The AZ-101 waste simulant and glass composition that was used for previous testing was selected for these tests.

Proceedings of the Annual Army Environmental R and D Symposium (15th) Held in Williamsburg, Virginia on 25-27 June 1991

Science.gov (United States)

1991-06-01

volatile and in sufficient quantity for the instrument to detect them. There are a number of mass spectro- meters on the market ; what makes the MM-i unique...standard calibration for analv., is r,e HS/GCTPID 1/10 Stock 10-80 mL 25 m 1-200 ucg Stock 50-200 rnL 2 rnL 100-3000 ue’- PT/GC/MS 1/10 Stock 10 rnL 100...requirement set by SAAD (<$1000 per gun without heater), they were readily available on the commercial market , and they were representative of the

Measurement of the isotope ratio of acetic acid in vinegar by HS-SPME-GC-TC/C-IRMS.

Science.gov (United States)

Hattori, Ryota; Yamada, Keita; Shibata, Hiroki; Hirano, Satoshi; Tajima, Osamu; Yoshida, Naohiro

2010-06-23

Acetic acid is the main ingredient of vinegar, and the worth of vinegar often depends on the fermentation of raw materials. In this study, we have developed a simple and rapid method for discriminating the fermentation of the raw materials of vinegar by measuring the hydrogen and carbon isotope ratio of acetic acid using head space solid-phase microextraction (HS-SPME) combined with gas chromatography-high temperature conversion or combustion-isotope ratio mass spectrometry (GC-TC/C-IRMS). The measurement of acetic acid in vinegar by this method was possible with repeatabilities (1sigma) of +/-5.0 per thousand for hydrogen and +/-0.4 per thousand for carbon, which are sufficient to discriminate the origin of acetic acid. The fermentation of raw materials of several vinegars was evaluated by this method.

Necesidad de circuncisión o dilatación del prepucio: Estudio de 1.200 niños

OpenAIRE

Morales Concepción, Julio C.; González Fernández, Pedro; Morales Aránegui, Aymée; Guerra Rodríguez, Marlen; Mora Casacó, Bárbara

2008-01-01

Objetivos: Demostrar el incremento de la retractilidad del prepucio con la edad. Señalar la poca utilidad de la circuncisión y la dilatación forzada del prepucio en la infancia. Métodos: El desarrollo del prepucio y su retractilidad, a medida que avanza la edad, fueron evaluados en 1200 niños de 0 a 16 años. El prepucio fue catalogado en tipos I a V según fue de menor a mayor su retractilidad en todos los casos que habían sido o no dilatados previamente. Resultados: La retractilidad del prepu...

Production of Ag Nanocubes on a Scale of 0.1 g per Batch by Protecting the NaHS-Mediated Polyol Synthesis with Argon

OpenAIRE

Zhang, Qiang; Cobley, Claire; Au, Leslie; McKiernan, Maureen; Schwartz, Andrea; Wen, Long-Ping; Chen, Jingyi; Xia, Younan

2009-01-01

Gold nanocages synthesized from Ag nanocubes via the galvanic replacement reaction are finding widespread use in a range of applications due to their tunable optical properties. Most of these applications require the use of nanocages with a uniform size and in large quantities. This requirement translates into a demand for scaling up the production of Ag nanocubes with uniform, well-controlled sizes. Here we report such a method based on the modification of NaHS-mediated polyol synthesis with...

Take-off analysis of the Olympic ski jumping competition (HS-106m).

Science.gov (United States)

Virmavirta, Mikko; Isolehto, Juha; Komi, Paavo; Schwameder, Hermann; Pigozzi, Fabio; Massazza, Giuseppe

2009-05-29

The take-off phase (approximately 6m) of the jumps of all athletes participating in the individual HS-106m hill ski jumping competition at the Torino Olympics was filmed with two high-speed cameras. The high altitude of the Pragelato ski jumping venue (1600m) and slight tail wind in the final jumping round were expected to affect the results of this competition. The most significant correlation with the length of the jump was found in the in-run velocity (r=0.628, pski jumping, and suggests that good jumpers simply had smaller friction between their skis and the in-run tracks and/or the aerodynamic quality of their in-run position was better. Angular velocity of the hip joint of the best jumpers was also correlated with jumping distance (r=0.651, pjumped approximately the same distance. This certainly improves the interests in ski jumping among athletes and spectators. The comparison between the take-off techniques of the best jumpers showed that even though the more marked upper body movement creates higher air resistance, it does not necessarily result in shorter jumping distance if the exposure time to high air resistance is not too long. A comparison between the first and second round jumps of the same jumpers showed that the final results in this competition were at least partly affected by the wind conditions.

Alleviation of Boron Stress through Plant Derived Smoke Extracts in Sorghum bicolor

Directory of Open Access Journals (Sweden)

Pirzada Khan

2014-08-01

Full Text Available Boron is an essential micronutrient necessary for plant growth at optimum concentration. However, at high concentrations boron affects plant growth and is toxic to cells. Aqueous extract of plant-derived smoke has been used as a growth regulator for the last two decades to improve seed germination and seedling vigor. It has been established that plant-derived smoke possesses some compounds that act like plant growth hormones. The present research was the first comprehensive attempt to investigate the alleviation of boron stress with plant-derived smoke aqueous extract on Sorghum (Sorghum bicolor seed. Smoke extracts of five plants, i.e. Cymbopogon jwarancusa, Eucalyptus camaldulensis, Peganum harmala, Datura alba and Melia azedarach each with six dilutions (Concentrated, 1:100, 1:200, 1:300, 1:400 and 1:500 were used. While boron solutions at concentrations of 5, 10, 15, 20 and 25 ppm were used for stress. Among the dilutions of smoke, 1:500 of E. camaldulensis significantly increased germination percentage, root and shoot length, number of secondary roots and fresh weight of root and shoot while, boron stress reduced growth of Sorghum. It was observed that combined effect of boron solution and E. camaldulensis smoke extract overcome inhibition and significantly improved plant growth. Present research work investigated that the smoke solution has the potential to alleviate boron toxicity by reducing the uptake of boron by maintaining integrity of plant cell wall. The present investigation suggested that plant derived smoke has the potential to alleviate boron stress and can be used to overcome yield losses caused by boron stress to plants.

Plasma Levels of High Sensitivity Cardiac Troponin T in Adults with Repaired Tetralogy of Fallot

Science.gov (United States)

Lai, Clare T. M.; Wong, Sophia J.; Ip, Janice J. K.; Wong, Wai-keung; Tsang, Kwong-cheong; Lam, Wendy W. M.; Cheung, Yiu-fai

2015-01-01

Detectable low circulating level of cardiac troponin T (cTnT) may reflect subclinical myocardial injury. We tested the hypothesis that circulating levels of hs-cTnT are altered in adults with repaired tetralogy of Fallot (TOF) and associated with ventricular volume load and function. Eighty-eight TOF patients and 48 controls were studied. Plasma hs-cTnT levels were determined using a highly sensitive assay (hs-cTnT). The right (RV) and left ventricular (LV) volumes and ejection fraction (EF) were measured using 3D echocardiography and, in 52 patients, cardiac magnetic resonance (CMR). The median (interquartile range) for male and female patients were 4.87 (3.83–6.62) ng/L and 3.11 (1.00–3.87) ng/L, respectively. Thirty percent of female but none of the male patients had increased hs-cTnT levels. Female patients with elevated hs-cTnT levels, compared to those without, had greater RV end-diastolic and end-systolic volumes and LV systolic dyssynchrony index (all p < 0.05). For patient cohort only, hs-cTnT levels correlated positively with CMR-derived RV end-diastolic volume and negatively with echocardiography-derived LV and RV EF (all p < 0.05). Multiple linear regression identified sex and RV EF as significant correlates of log-transformed hs-cTnT levels. Increased hs-cTnT levels occur in 30% of female patients after TOF repair, and are associated with greater RV volumes and worse RV EF. PMID:26360613

Numerical investigation of passive heat removal system via steam generator in VVER 1200

International Nuclear Information System (INIS)

Dinh Anh Tuan; Duong Thanh Tung; Tran Chi Thanh; Nguyen Van Thai

2015-01-01

Passive heat removal system (PHRS) via Steam Generator is an important part in VVER design. In case of Design Basic Accidents such as blackout, failure of feed water supply to steam generator or coolant leakage with failure of emergency core cooling at high pressure. PHRS is designed to remove the residual heat from reactor core through steam generator to heat exchanger which is placed outside reactor vessel. In order to evaluate the passive system, a numerical investigation using a CFD code is performed. However, PHRS has complex geometry for using CFD simulation. Thus, RELAP5 is applied to provide the wall heat flux of tube in the heat exchanger tank. The natural convection in the heat exchanger tank is investigated in this report. Numerical results show temperature and velocity distribution in the heat exchanger tank are calculated with different wall heat flux corresponding to various transient conditions. The calculated results contribute to the capacity analysis of passive heat removal system and giving valuable information for safe operation of VVER 1200. (author)

Capital cost: high and low sulfur coal plants-1200 MWe. [High sulfur coal

Energy Technology Data Exchange (ETDEWEB)

1977-01-01

This Commercial Electric Power Cost Study for 1200 MWe (Nominal) high and low sulfur coal plants consists of three volumes. The high sulfur coal plant is described in Volumes I and II, while Volume III describes the low sulfur coal plant. The design basis and cost estimate for the 1232 MWe high sulfur coal plant is presented in Volume I, and the drawings, equipment list and site description are contained in Volume II. The reference design includes a lime flue gas desulfurization system. A regenerative sulfur dioxide removal system using magnesium oxide is also presented as an alternate in Section 7 Volume II. The design basis, drawings and summary cost estimate for a 1243 MWe low sulfur coal plant are presented in Volume III. This information was developed by redesigning the high sulfur coal plant for burning low sulfur sub-bituminous coal. These coal plants utilize a mechanical draft (wet) cooling tower system for condenser heat removal. Costs of alternate cooling systems are provided in Report No. 7 in this series of studies of costs of commercial electrical power plants.

Collisional-Radiative Modeling of Tungsten at Temperatures of 1200–2400 eV

Directory of Open Access Journals (Sweden)

James Colgan

2015-04-01

Full Text Available We discuss new collisional-radiative modeling calculations of tungsten at moderate temperatures of 1200 to 2400 eV. Such plasma conditions are relevant to ongoing experimental work at ASDEX Upgrade and are expected to be relevant for ITER. Our calculations are made using the Los Alamos National Laboratory (LANL collisional-radiative modeling ATOMIC code. These calculations formed part of a submission to the recent NLTE-8 workshop that was held in November 2013. This series of workshops provides a forum for detailed comparison of plasma and spectral quantities from NLTE collisional-radiative modeling codes. We focus on the LANL ATOMIC calculations for tungsten that were submitted to the NLTE-8 workshop and discuss different models that were constructed to predict the tungsten emission. In particular, we discuss comparisons between semi-relativistic configuration-average and fully relativistic configuration-average calculations. We also present semi-relativistic calculations that include fine-structure detail, and discuss the difficult problem of ensuring completeness with respect to the number of configurations included in a CR calculation.

Electrosynthesis of Clozapine Drug Derivative via an EC Electrochemical Mechanism

Directory of Open Access Journals (Sweden)

Esmail Tammari

2017-12-01

Full Text Available The fact that oxidation, as one of the main routes of phase I metabolism of drugs, follows by conjugation reactions, and also formation of nitrenium ion as one of the clozapine oxidation products, directed us to investigate the oxidation of clozapine (CLZ in the presence of nucleophile. The oxidation of clozapine (CLZ has been studied on a glassy carbon electrode in the absence and presence of 2-thiobarbituric acid (TBA as nucleophile in aqueous medium by means of cyclic voltammetry and on the graphite rods in controlled-potential coulometry. Cyclic voltammetry studies were realized for CLZ in the pHs 1.0 to 8.0. Results indicate that the electrochemical behavior of CLZ depends on the pH. Based on the obtained electrochemical results, an ECE mechanism was proposed to explain the electrochemical oxidation of CLZ. The results revealed that oxidized CLZ participates in Michael type addition reaction with TBA and via an EC mechanism converts to the corresponding new dibenzodiazepin derivatives. The product has been characterized by IR, 1H NMR, 13C NMR and MS.

Quantitative proteomics reveals altered expression of extracellular matrix related proteins of human primary dermal fibroblasts in response to sulfated hyaluronan and collagen applied as artificial extracellular matrix.

Science.gov (United States)

Müller, Stephan A; van der Smissen, Anja; von Feilitzsch, Margarete; Anderegg, Ulf; Kalkhof, Stefan; von Bergen, Martin

2012-12-01

Fibroblasts are the main matrix producing cells of the dermis and are also strongly regulated by their matrix environment which can be used to improve and guide skin wound healing processes. Here, we systematically investigated the molecular effects on primary dermal fibroblasts in response to high-sulfated hyaluronan [HA] (hsHA) by quantitative proteomics. The comparison of non- and high-sulfated HA revealed regulation of 84 of more than 1,200 quantified proteins. Based on gene enrichment we found that sulfation of HA alters extracellular matrix remodeling. The collagen degrading enzymes cathepsin K, matrix metalloproteinases-2 and -14 were found to be down-regulated on hsHA. Additionally protein expression of thrombospondin-1, decorin, collagen types I and XII were reduced, whereas the expression of trophoblast glycoprotein and collagen type VI were slightly increased. This study demonstrates that global proteomics provides a valuable tool for revealing proteins involved in molecular effects of growth substrates for further material optimization.

Table of charged particle energies versus magnetic field strength x orbit radius (B{rho}) for A = 1 to 7 (100< (B{rho}) < 1200 kG.cm); Table des energies des particules chargees en fonction de la rigidite magnetique (B{rho}) pour A = 1 a 7 (100< (B{rho}) < 1200 kG.cm)

Energy Technology Data Exchange (ETDEWEB)

Bianchi, L. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1969-07-01

A table of charged particle energies versus magnetic field strength x orbit radius (B{sub {rho}}) is presented. Particles p, d, t, {sup 3}He{sup ++}, {sup 4}He{sup +}, {sup 4}He{sup ++}, {sup 6}Li{sup +}, {sup 6}Li{sup ++}, {sup 6}Li{sup +++}, {sup 7}Li{sup +}, {sup 7}Li{sup ++}, {sup 7}Li{sup +++}. Values of B{sub {rho}}: 100 to 1200 kG.cm by steps of 0.5 kG.cm. Values of energies are given in keV. (author) [French] Nous presentons une table des energies de protons, deutons, tritons, {sup 3}He{sup ++}, {sup 4}He{sup +}, {sup 4}He{sup ++}, {sup 6}Li{sup +}, {sup 6}Li{sup ++}, {sup 6}Li{sup +++}, {sup 7}Li{sup +}, {sup 7}Li{sup ++}, {sup 7}Li{sup +++} en fonction de leur rigidite magnetique (B{sub {rho}}). Les valeurs de B{sub {rho}} sont comprises entre 100 et 1200 kG.cm par pas de 0.5 kG.cm. Les valeurs des energies sont donnees en keV. (auteur)

Oxidation of Zircaloy-4 in steam-nitrogen mixtures at 600–1200 °C

Energy Technology Data Exchange (ETDEWEB)

Steinbrueck, Martin, E-mail: martin.steinbrueck@kit.edu; Oliveira da Silva, Fabio; Grosse, Mirco

2017-07-15

High-temperature oxidation of zirconium alloys in steam-nitrogen atmospheres may be relevant during various nuclear accident scenarios. Therefore, isothermal oxidation tests with Zircaloy-4 in steam-nitrogen mixtures have been performed at 600, 800, 1000, and 1200 °C using thermogravimetry. The gas compositions were varied between 0 and 100 vol% nitrogen including 0.1 and 90 vol%. The strong effect of nitrogen on the oxidation kinetics of zirconium alloys was confirmed in these tests in mixed steam-nitrogen atmospheres. Even very low concentrations of nitrogen (starting from less than 1 vol%) strongly increase reaction kinetics. Nitrogen reduces transition time from protective to non-protective oxide scale (breakaway). The formation of zirconium nitride, ZrN, and its re-oxidation is the main reason for the highly porous oxide scales after transition. The results of this study have shown the safety relevant role of nitrogen during severe accidents and, more generally, suggest the need of using well controlled gas atmospheres for experiments on oxidation of zirconium alloys.

Physical startup tests for VVER-1200 of Novovoronezh NPP. Advanced technique and some results

Energy Technology Data Exchange (ETDEWEB)

Afanasiev, Dmitry A.; Kraynov, Yury A.; Pinegin, Anatoly A.; Tsyganov, Sergey V. [National Research Centre, Moscow (Russian Federation). Kurchatov Inst.

2017-09-15

The intention of the startup physics tests was to confirm design characteristics of the core loading and their compliance with safety analysis preconditions. The program of startup tests for the leading unit is usually composed in such a way that is is possible to study as much neutron-physical characteristics as possible in the safest condition of zero power. State-of-the-art safety analysis is including computer codes that use three dimensional neutron kinetics and thermohydraulics models. For the substantiation of such models, for its validation and verification there is a need in reactor experiments that implementing spatially distributed transients. We based on such statements when composing hot zero power physical startup program for the new VVER-1200 unit of Novovoronezh NPP. Several tests unconventional for VVER were developed for that program. It includes measuring the worth for each of control rod groups and measuring of single rod worth from the inserted groups - test that models rod ejection event in some sense.

Quantitative measurement of a candidate serum biomarker peptide derived from α2-HS-glycoprotein, and a preliminary trial of multidimensional peptide analysis in females with pregnancy-induced hypertension.

Science.gov (United States)

Hamamura, Kensuke; Yanagida, Mitsuaki; Ishikawa, Hitoshi; Banzai, Michio; Yoshitake, Hiroshi; Nonaka, Daisuke; Tanaka, Kenji; Sakuraba, Mayumi; Miyakuni, Yasuka; Takamori, Kenji; Nojima, Michio; Yoshida, Koyo; Fujiwara, Hiroshi; Takeda, Satoru; Araki, Yoshihiko

2018-03-01

Purpose We previously attempted to develop quantitative enzyme-linked immunosorbent assay (ELISA) systems for the PDA039/044/071 peptides, potential serum disease biomarkers (DBMs) of pregnancy-induced hypertension (PIH), primarily identified by a peptidomic approach (BLOTCHIP®-mass spectrometry (MS)). However, our methodology did not extend to PDA071 (cysteinyl α2-HS-glycoprotein 341-367 ), due to difficulty to produce a specific antibody against the peptide. The aim of the present study was to establish an alternative PDA071 quantitation system using liquid chromatography-multiple reaction monitoring (LC-MRM)/MS, to explore the potential utility of PDA071 as a DBM for PIH. Methods We tested heat/acid denaturation methods in efforts to purify serum PDA071 and developed an LC-MRM/MS method allowing for specific quantitation thereof. We measured serum PDA071 concentrations, and these results were validated including by three-dimensional (3D) plotting against PDA039 (kininogen-1 439-456 )/044 (kininogen-1 438-456 ) concentrations, followed by discriminant analysis. Results PDA071 was successfully extracted from serum using a heat denaturation method. Optimum conditions for quantitation via LC-MRM/MS were developed; the assayed serum PDA071 correlated well with the BLOTCHIP® assay values. Although the PDA071 alone did not significantly differ between patients and controls, 3D plotting of PDA039/044/071 peptide concentrations and construction of a Jackknife classification matrix were satisfactory in terms of PIH diagnostic precision. Conclusions Combination analysis using both PDA071 and PDA039/044 concentrations allowed PIH diagnostic accuracy to be attained, and our method will be valuable in future pathophysiological studies of hypertensive disorders of pregnancy.

Lemon detox diet reduced body fat, insulin resistance, and serum hs-CRP level without hematological changes in overweight Korean women.

Science.gov (United States)

Kim, Mi Joung; Hwang, Jung Hyun; Ko, Hyun Ji; Na, Hye Bock; Kim, Jung Hee

2015-05-01

The lemon detox program is a very low-calorie diet which consists of a mixture of organic maple and palm syrups, and lemon juice for abstinence period of 7 days. We hypothesized that the lemon detox program would reduce body weight, body fat mass, thus lowering insulin resistance and known risk factors of cardiovascular disease. We investigated anthropometric indices, insulin sensitivity, levels of serum adipokines, and inflammatory markers in overweight Korean women before and after clinical intervention trial. Eighty-four premenopausal women were randomly divided into 3 groups: a control group without diet restriction (Normal-C), a pair-fed placebo diet group (Positive-C), and a lemon detox diet group (Lemon-D). The intervention period was 11 days total: 7 days with the lemon detox juice or the placebo juice, and then 4 days with transitioning food. Changes in body weight, body mass index, percentage body fat, and waist-hip ratio were significantly greater in the Lemon-D and Positive-C groups compared to the Normal-C group. Serum insulin level, homeostasis model assessment insulin resistance scores, leptin, and adiponectin levels decreased in the Lemon-D and Positive-C groups. Serum high-sensitive C-reactive protein (hs-CRP) levels were also reduced only in the Lemon-D group. Hemoglobin and hematocrit levels remained stable in the Lemon-D group while they decreased in the Positive-C and Normal-C groups. Therefore, we suppose that the lemon detox program reduces body fat and insulin resistance through caloric restriction and might have a potential beneficial effect on risk factors for cardiovascular disease related to circulating hs-CRP reduction without hematological changes. Copyright © 2015 Elsevier Inc. All rights reserved.

Pilates versus resistance exercise on the serum levels of hs-CRP, in the abdominal circumference and body mass index (BMI) in elderly individuals

OpenAIRE

Pestana, Maria Dida Silva; Netto, Eduardo Martins; Pestana, Manuella Castro Silva; Pestana, Vitor Silva; Schinoni, Maria Isabel

2016-01-01

Recent studies have shown that the elderly exhibit a subclinical state of inflammation associated with increased adipose tissue and several comorbidities. To compare the effects of mat Pilates based exercises and resistance exercise on the serum levels of reactive C protein of high sensitivity (hs-CRP), in the abdominal circumference (AC) and the body mass index (BMI) in the elderly. It is a randomised clinical trial with a sample of 78 elderly individuals (median age 69 years). The active in...

HS-GC-MS-O analysis and sensory acceptance of passion fruit during maturation.

Science.gov (United States)

Janzantti, Natália S; Monteiro, Magali

2017-07-01

The odor-active compounds of the conventional yellow passion fruit influence the aroma during ripeness and the acceptance of the juice. HS-GC-MS and GC-OSME analysis and sensory acceptance of the conventional passion fruit from different stages of ripeness were studied to characterize the aroma of the fruit and, aroma and flavor of the juice. Ethyl butanoate, ethyl hexanoate and propyl acetate showed high odoriferous importance in the passion fruit from the 1/3 yellow skin color. C is -3-hexen-1-ol and diethyl carbonate plus the odor-active compounds from the 1/3 yellow skin color showed high odoriferous importance in the 2/3 yellow skin color, and butyl acetate and alpha-terpineol plus the same odor-active compounds from 2/3 were the most important for the 3/3 yellow skin color. There was difference in the aroma and flavor of the juices, with higher acceptance means for the passion fruit from the 3/3 yellow skin color. The passion fruit volatile compounds peak area, odoriferous intensity and sensory acceptance of the juices increased during ripeness, indicating that the conventional passion fruit characteristic aroma is completely expressed when the fruit reaches the whole maturation, at the 3/3 yellow skin color.

Mullite evolution in ceramics derived from clay, and sol-gel precursors

International Nuclear Information System (INIS)

Rezaie, H.R.; Naghizadeh, R.; Golestani-Fard, F.

2002-01-01

Mullite formation from sol-gel (behemoth and colloidal silica), and clay has been compared and the microstructural evolution examined using electron-optical techniques and XRD. The relationship between processing route and reaction sequence to form mullite is discussed. In sol-gel processing γAloof transforms to γ-Al 2 O 3 at 400-500 d eg w ithout an isothermal hold. δ-Al 2 O 3 was present after cooling directly from 1100 d eg i n air.(δ+θ)Al 2 O 3 were present above 1200 d eg . Reaction between (δ+θ)Al 2 O 3 and amorphous silica produced 3:2 mullite at∼ 1370 d eg . In kaolin, meta kaolin transforms to spinel and mullite at 980-1000 d eg w ithout an isothermal hold. Melanostatin to 3:2 mullite took place at 1200-1250 d eg . Cristobalite was observed after quenching from 1200 d eg , but could not be detected after cooling directly from 1590 d eg

Transport of heparan sulfate into the nuclei of hepatocytes

International Nuclear Information System (INIS)

Ishihara, M.; Fedarko, N.S.; Conrad, H.E.

1986-01-01

A rat hepatocyte cell line which accumulates free heparan sulfate (HS) chains enriched in GlcA-2-SO 4 residues in the nucleus was labeled with 35 SO 4 2- and the rate of appearance of [ 35 SO 4 ]HS in the nucleus was measured. [ 35 SO 4 ]HS began to accumulate in the nucleus 2 h after the addition of 35 SO 42- and reached a steady state level after 20 h. HS was lost from the nuclei of prelabeled cells with a t/sub 1/2/ of 8 h. Chloroquine did not inhibit the transport of HS into the nucleus, but increased the t/sub 1/2/ for the exit of HS from the nucleus to 20 h. At both 37 0 C and 16 0 C exogenous [ 35 SO 4 ]proteoHS was taken up by the cells and converted to free chains and about 10% of the internalized [ 35 SO 4 ]HS was transported into the nucleus. The [ 35 SO 4 ]HS isolated from the nucleus was enriched in GlcA-2-SO 4 residues, whereas the [ 35 SO 4 ]HS remaining in the rest of the intra-cellular pool showed a corresponding depletion in GlcA-2-SO 4 residues. The results show that nuclear HS is derived from the pool of a secreted proteoHS and that metabolism of exogenous HS by hepatocytes does not involve lysosomal processing of the internalized HS

The S-matrix for abstract scattering systems

International Nuclear Information System (INIS)

Amrein, W.O.; Pearson, D.B.

1979-01-01

Let S(lambda) be the S-matrix at energy lambda for an abstract scattering system. A bound is derived in terms of the interaction, on integrals of the form ∫ h(lambda)/S(lambda) - I/ 2 sub(HS) dlambda, where /./sub(HS) denotes the Hilbert-Schmidt norm. (Auth.)

Promoting effect of bile acids on neoplastic transformation of x-irradiated 10T1/2 cells

International Nuclear Information System (INIS)

Han, A.; Hill, C.K.

1984-01-01

Experimental studies have raised a concern about a role of bile acids in colo-rectal carcinogenesis. Studies in vivo suggest that bile acids may act as tumor promoters. Using 10T1/2 mouse cells as a model system, the authors explored the effects of cholic and cheno-deoxycholic acid on x-ray-induced neoplastic transformation in these cells. Addition of either cheno-deoxycholic acid or cholic acid to 10T1/2 cells, 24 hours after exposure to x-rays (50kv) increases significantly the frequencies of transformation. The compounds were present in the medium throughout the entire postirradiation refeeding period. At the concentrations used (0.5μg/ml), neither acid was cytotoxic and did not have any effect on cell survival. The enhancement of radiation-induced transformation seems to be greater in the presence of cholic acid, as compared to the effect of cheno-deoxycholic acid. Increase in transformation was relatively greater after low compared to high doses of radiation. The effect of bile acids on transformation of 10T1/2 cells is similar to that of a known tumor promoter TPA. The authors' observations support the conclusion that promotional effect of bile acids is not because of their specific effect on colonic epithelium, but rather due to their general properties as tumor promoters

Design Evaluation of UIS and In-vessel Fuel Transfer Machine for a 1200MWe SFR

Energy Technology Data Exchange (ETDEWEB)

Lee, Jae Han; Kim, Seok Hoon; Park, Chang Gyu; Lee, Su Yeon

2008-11-15

The report describes the structural applicability of the upper internal structure (UIS) and the in-vessel fuel transfer machine for a 1200MWe sodium cooled fast reactor (SFR) of a pool type. In the conceptual design, a two rotating plug type as a refueling system is considered. For the two rotating plug type, the diameters of large and small rotating plugs are determined by 7.2m and 5.6m, respectively. Through the use of an inner fuel transfer machine and the lift change machine with a fixed arm length of 1.10m installed on a small rotating plug, all the core assemblies are accessed within 7mm accuracy. The UIS diameter is determined by 4.7m, which includes the all control drive lines in upper part, the diameter of UIS lower part is restricted by 4.4 m to secure the rotation angle of a refueling machine.

Chenodeoxycholic acid stimulated fibroblast growth factor 19 response

DEFF Research Database (Denmark)

Borup, C; Wildt, S; Rumessen, J J

2017-01-01

BACKGROUND: Bile acid diarrhoea is underdiagnosed and better diagnostic tests are needed. Fasting serum fibroblast growth factor-19 (FGF19) has insufficient diagnostic value, but this may be improved by stimulation. AIM: To explore if an impaired FGF19 response identifies primary bile acid...

Comparison of rigid and swivelling rotor hubs for a MW wind power system, using the example of the WKA Autoflug A 1200; Vergleich von starrer und Pendel-Rotornabe fuer eine Windkraftanlage der Megawatt-Leistungsklasse am Beispiel der WKA Autoflug A 1200

Energy Technology Data Exchange (ETDEWEB)

Quell, P. [aerodyn Energiesysteme GmbH, Rendsburg (Germany)

1996-12-31

Developments in the MW range are a further illustration of the well-known problem that a linear increase of the rotor surface area will result in an overproportional increase of mass. One classical approach for reducing weight is the installation of a two-blade rotor with a swivelling hub. In the course of the development activities for the 1.2 MW A1200 wind power system of Autoflug Energietechnik GmbH, it was investigated whether a pendulum hub is a technically and economically feasible concept for a plant of this size. For this purpose, two otherwise identical plants were projected and compared, one with a rigid hub and the other with a swivelling hub. Both systems were simulated using the dynamic program GAROS. With the loads thus determined, both concepts are detailed enough to permit a comprehensive cost comparison in consideration of the masses, the resulting cost, performance and reliability. [Deutsch] Der Entwicklungsprozess der Megawatt-Anlagen verdeutlicht den aus frueheren Untersuchungen bekannten Zusammenhang eines ueberproportionalen Massenzuwachses bei linear steigender Rotorflaeche. Ein klassischer Ansatz zur Reduzierung des Anlagengewichtes ist der Einsatz eines Zweiblatt-Rotors in Verbindung mit einer Pendelnabe. Im Rahmen der Entwicklungstaetigkeit fuer die 1,2 MW-Windkraftanlage A 1200 der Fa. Autoflug Energietechnik GmbH wurde untersucht, inwieweit der Einsatz einer Pendelnabe fuer eine Anlage dieser Groessenordnung ein technisch und wirtschaftlich sinnvolles Konzept darstellen kann. Dazu wurden zwei grundsaetzlich gleiche Anlagen mit Zweiblatt-Rotor unter Verwendung einer Pendelnabe und einer starren Nabe konzipiert und gegenuebergestellt. Zur Bewertung des Anlagenverhaltens sowie zur Ermittlung realitaetsnaher Belastungen werden sowohl die Anlagenversion mit Pendelnabe als auch die Version mit starrer Nabe mit dem Dynamikprogramm GAROS simuliert. Basierend auf den ermittelten Lasten werden beide Konzepte so detailliert, dass ein umfassender

Spironolactone release from liquisolid formulations prepared with Capryol™ 90, Solutol® HS-15 and Kollicoat® SR 30 D as non-volatile liquid vehicles.

Science.gov (United States)

Elkordy, Amal Ali; Tan, Xin Ning; Essa, Ebtessam Ahmed

2013-02-01

The purpose of the study is to enhance dissolution of spironolactone as a model hydrophobic drug through application of liquisolid technology. Spironolactone is prepared as liquisolid formulations, and its dissolution property is evaluated and compared to that of conventional spironolactone tablets and pure spironolactone. Three non-volatile liquid vehicles were used in the design of spironolactone liquisolid formulations, Capryol™ 90, Synperonic® PE/L61 in combination with Solutol® HS-15 at a ratio of 1:1, and Kollicoat® SR 30 D. Spironolactone liquisolid formulations were tested according to British Pharmacopoeia (BP) quality control tests. Furthermore, the prepared liquisolid powder formulations were evaluated via differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) and scanning electron microscopy. Also, liquisolid formulations were subjected to testing of storage stability at high relative humidity. The results indicated that most of liquisolid tablets met the BP requirements. Dissolution results indicate that release of spironolactone was significantly increased (PSolutol® HS-15 showed highest dissolution. DSC thermograms from liquisolid formulations revealed that drug endothermic peak was disappeared after processing. Dissolution, DSC and FT-IR data after storage demonstrated that there were no significant changes in the formulations after storage. In conclusion, the liquid vehicles used within spironolactone liquisolid formulations enhanced drug dissolution rate. Copyright © 2012 Elsevier B.V. All rights reserved.

Electrochemical and spectroscopic studies of the complexed species of models of nitrohumic acids derived from phthalic acid

Directory of Open Access Journals (Sweden)

Mercê Ana Lucia Ramalho

1998-01-01

Full Text Available The study of model compounds is necessary in order to obtain information about complex organic substances as in the case of humic substances (HS. These substances are potential organic fertilizers and have other important functions in soils, natural waters and organic sediments. The main chemical properties of the complexes formed from 3-nitrophthalic and 4-nitrophthalic acids and the metal ions Fe(III and Zn(II were studied using potentiometric titrations, ultraviolet-visible spectroscopy (UV-Vis and cyclic voltammetry (CV. A trial potentiometric titration was done with a mixture of the models for nitrohumic acids and Cu(II. Equilibrium constants for the systems were calculated and UV-Vis and CV were employed to monitor the formation of the species. Comparative studies involving chelating centres of nitrosalicylic acids and nitrocatechols with Fe(III, Zn(II and Cu(II are presented. The initial studies involving the nitrohumic substances (NHS, a laboratory artifact of HS have been made and good evidence was found for the further use of NHS as a potential organic fertilizer as well as HS. In this present work one of the observed advantages of NHS over HS was that some aromatic nitro- centres can bind some metal ions at p[H] values of normal soils, near 7.0 to 7.5.

Effects of synbiotic food consumption on glycemic status and serum hs-CRP in pregnant women: a randomized controlled clinical trial.

Science.gov (United States)

Taghizadeh, Mohsen; Asemi, Zatolla

2014-01-01

The aim of this study was to determine the effects of synbiotic food consumption on glycemic status and serum high sensitivity C-reactive protein (hs-CRP) levels of Iranian pregnant women. This randomized placebo-controlled clinical trial was performed among 52 pregnant women, primigravida, aged 18-35 year old, in their third trimester. After a 2-wk run-in period, subjects were randomly assigned to consume either a synbiotic (n=26) or control food (n=26) for 9 weeks. The synbiotic food consisted of a probiotic Lactobacillus sporogenes (1×107 CFU), 0.04 g inulin as prebiotic with 0.38 g isomalt, 0.36 g sorbitol and 0.05 g stevia as sweetener per 1 g. Control food (the same substance without probiotic bacteria and inulin) was packed in identical 9-gram packages. Patients were asked to consume the synbiotic and control foods two times a day. Fasting blood samples were taken at baseline and after a 9-wk intervention for quantification of related factors. Consumption of a synbiotic food did not show any significant change regarding the impact of insulin actions in the synbiotic group; nonetheless, compared to the control food, it resulted in a significant decrease in serum insulin levels (-0.26 vs. 6.34 µIU/mL, P=0.014) and HOMA-IR (-0.13 vs. 1.13, P=0.033), a significant difference in HOMA-B (5.30 vs. 34.22, P=0.040) and a significant rise in QUICKI score (0.002 vs. -0.02, P=0.022). Consumption of a synbiotic food for 9 weeks by pregnant women had beneficial effects on insulin actions compared to the control food, but did not affect FPG and serum hs-CRP concentrations.

Effects of Silybum marianum (L.) Gaertn. (silymarin) extract supplementation on antioxidant status and hs-CRP in patients with type 2 diabetes mellitus: a randomized, triple-blind, placebo-controlled clinical trial.

Science.gov (United States)

Ebrahimpour Koujan, Soraiya; Gargari, Bahram Pourghassem; Mobasseri, Majid; Valizadeh, Hadi; Asghari-Jafarabadi, Mohammad

2015-02-15

Diabetes is a serious metabolic disorder and oxidative stress and inflammation contribute to its pathogenesis and complications. Since Silybum marianum (L.) Gaertn. (silymarin) extract is an antioxidant with anti-inflammatory properties, this randomized clinical trial was conducted to evaluate the effects of silymarin supplementation on oxidative stress indices and hs-CRP in type 2 diabetes mellitus patients. For the present paralleled, randomized, triple-blinded, placebo-controlled clinical trial, 40 type 2 diabetes patients aged 25-50 yr old and on stable medication were recruited from the Iranian Diabetes Society and endocrinology clinics in East Azarbayjan (Tabriz, Iran) and randomly assigned into two groups. Patients in the silymarin treatment group received 140 mg, thrice daily of dried extracts of Silybum marianum (n = 20) and those in the placebo group (n = 20) received identical placebos for 45 days. Data pertaining to height, weight, waist circumference and BMI, as well as food consumption, were collected at base line and at the conclusion of the study. Fasting blood samples were obtained and antioxidant indices and hs-CRP were assessed at baseline, as well as at the end of the trial. All 40 patients completed the study and did not report any adverse effects or symptoms with the silymarin supplementation. Silymarin supplementation significantly increased superoxide dismutase (SOD), glutathione peroxidase (GPX) activity and total antioxidant capacity (TAC) compared to patients taking the placebo, by 12.85%, 30.32% and 8.43%, respectively (p concentration significantly decreased by 12.01% (p antioxidant indices (SOD, GPX and TAC) and decrease hs-CRP levels in T2DM patients. Copyright © 2015. Published by Elsevier GmbH.

Peripheral brain-derived neurotrophic factor is related to cardiovascular risk factors in active and inactive elderly men

Directory of Open Access Journals (Sweden)

A. Zembron-Lacny

2016-01-01

Full Text Available Regular exercise plays an important preventive and therapeutic role in heart and vascular diseases, and beneficially affects brain function. In blood, the effects of exercise appear to be very complex and could include protection of vascular endothelial cells via neurotrophic factors and decreased oxidative stress. The purpose of this study was to identify the age-related changes in peripheral brain-derived neurotrophic factor (BDNF and its relationship to oxidative damage and conventional cardiovascular disease (CVD biomarkers, such as atherogenic index, C-reactive protein (hsCRP and oxidized LDL (oxLDL, in active and inactive men. Seventeen elderly males (61-80 years and 17 young males (20-24 years participated in this study. According to the 6-min Åstrand-Rhyming bike test, the subjects were classified into active and inactive groups. The young and elderly active men had a significantly better lipoprotein profile and antioxidant status, as well as reduced oxidative damage and inflammatory state. The active young and elderly men had significantly higher plasma BDNF levels compared to their inactive peers. BDNF was correlated with VO2max (r=0.765, P<0.001. In addition, we observed a significant inverse correlation of BDNF with atherogenic index (TC/HDL, hsCRP and oxLDL. The findings demonstrate that a high level of cardiorespiratory fitness reflected in VO2max was associated with a higher level of circulating BDNF, which in turn was related to common CVD risk factors and oxidative damage markers in young and elderly men.

Aqueous poly(amidoamine) dendrimer G3 and G4 generations with several interior cores at pHs 5 and 7: a molecular dynamics simulation study.

Science.gov (United States)

Kavyani, Sajjad; Amjad-Iranagh, Sepideh; Modarress, Hamid

2014-03-27

Poly(amidoamine) (PAMAM) dendrimers play an important role in drug delivery systems, because the dendrimers are susceptible to gain unique features with modification of their structure such as changing their terminals or improving their interior core. To investigate the core improvement and the effect of core nature on PAMAM dendrimers, we studied two generations G3 and G4 PAMAM dendrimers with the interior cores of commonly used ethylendiamine (EDA), 1,5-diaminohexane (DAH), and bis(3-aminopropyl) ether (BAPE) solvated in water, as an aqueous dendrimer system, by using molecular dynamics simulation and applying a coarse-grained (CG) dendrimer force field. To consider the electrostatic interactions, the simulations were performed at two protonation states, pHs 5 and 7. The results indicated that the core improvement of PAMAM dendrimers with DAH produces the largest size for G3 and G4 dendrimers at both pHs 5 and 7. The increase in the size was also observed for BAPE core but it was not so significant as that for DAH core. By considering the internal structure of dendrimers, it was found that PAMAM dendrimer shell with DAH core had more cavities than with BAPE core at both pHs 5 and 7. Also the moment of inertia calculations showed that the generation G3 is more open-shaped and has higher structural asymmetry than the generation G4. Possessing these properties by G3, specially due to its structural asymmetry, make penetration of water beads into the dendrimer feasible. But for higher generation G4 with its relatively structural symmetry, the encapsulation efficiency for water molecules can be enhanced by changing its core to DAH or BAPE. It is also observed that for the higher generation G4 the effect of core modification is more profound than G3 because the core modification promotes the structural asymmetry development of G4 more significantly. Comparing the number of water beads that penetrate into the PAMAM dendrimers for EDA, DAH, and BAPE cores indicates a

Table of charged particle energies versus magnetic field strength x orbit radius (Bρ) for A = 1 to 7 (100< (Bρ) < 1200 kG.cm)

International Nuclear Information System (INIS)

Bianchi, L.

1969-01-01

A table of charged particle energies versus magnetic field strength x orbit radius (B ρ ) is presented. Particles p, d, t, 3 He ++ , 4 He + , 4 He ++ , 6 Li + , 6 Li ++ , 6 Li +++ , 7 Li + , 7 Li ++ , 7 Li +++ . Values of B ρ : 100 to 1200 kG.cm by steps of 0.5 kG.cm. Values of energies are given in keV. (author) [fr

IUE observations of the hot components in two symbiotic stars. [R Agr and RW Hya, 1200 to 3200 A

Energy Technology Data Exchange (ETDEWEB)

Michalitsianos, A G [National Aeronautics and Space Administration, Greenbelt, MD (USA). Goddard Space Flight Center; Kafatos, M; Hobbs, R W; Maran, S P

1980-03-13

Ultraviolet measurements in the 1,200 - 3,200 A range are reported on two symbiotic stars, R Agr and RW Hya. The results indicate the presence of a hot component in each star, supporting the view that each is a binary system with a luminous red primary and a hot, sub-luminous companion. In the case of RW Hya the hot companion manifests itself by exciting a compact nebulosity while in the case of R Agr it is believed that the continuous spectrum of the hot star is directly detected, while the continuum of nebulosity excited by the hot star is detected at longer wavelengths.

Safety Design Criteria and Approaches to Safety Substantiation of the BN-1200

International Nuclear Information System (INIS)

Ashurko, I.

2013-01-01

Russian experience in SFR area: Activities on development of safety design criteria for SFRs of the 4th generation is carried out within the GIF framework. Although this reactor technology is considered as innovative that is relevant to the 4th generation, however, it has already a certain history. In this relation, it seems to be useful to analyze the corresponding experience that is available in various countries. 4 SFRs have been successfully operated in the USSR and in the Russian Federation: • Experimental reactor BR-5/10; • Research reactor BOR-60; • Prototype BN-350 power reactor; • Commercial BN-600 power unit at the Beloyarsk NPP. Thus, Russia gained a considerable experience of design, construction and operation of SFRs. In particular, a certain experience has been acquired on safety substantiation of reactors of this type and their licensing. Now BOR-60 and BN-600 continue their operation, BN-800 power unit is under construction, development of the commercial BN-1200 power unit, that is considered as the 4th generation reactor, has been started. Due to limited number of operating SFRs in the world, successful Russian experience in this area should be taken into account for further development and improvement of SFR SDC developed by the GIF Task Force. In particular, participation of SFR designers in this activities would be fruitful and useful

Evaluation of VVER-1200/V-491 reactor pressure vessel integrity during large break LOCA along with SBO using MELCOR 1.8.6

International Nuclear Information System (INIS)

Bui Thi Hoa; Tran Chi Thanh

2015-01-01

After Fukushima accident and stress test recommended by IAEA for existing reactors, higher safety requirements are enforced upon nuclear power plants during design extension and severe accident conditions. Based on those arguments, Vietnam Government requests a lot of effective safety solutions, in designs proposed for the nuclear power plants in Ninh Thuan province of Vietnam, which can prevent the accident progression toward severe accidents and mitigate severe accident consequences. One of safety requirements is related to delay time of core melt during design extension condition. Especially, if the worst case of accidents occurs, the reactor vessel integrity must be maintained at least 24 hours from the beginning of the accident. With the aim at investigation of Reactor Pressure Vessel (RPV) integrity, in this study, MELCOR 1.8.6 code is used to evaluate the integrity of RPV lower head for VVER-1200/V-491 reactor during a Large Break Loss of Coolant Accident (LBLOCA) in combination with Station Blackout (SBO) event. The study figures out several parameters related to melt down progress such as: rupture position and rupture timing, the amount of hydrogen generated. Availability of the second stage hydro-accumulators (HA2) in the VVER-1200/V-491 is assumed as an additional improvement to delay the timing of core melt as well as to maintain the vessel integrity for long-term. (author)

Analysis of Five Earthy-Musty Odorants in Environmental Water by HS-SPME/GC-MS

Directory of Open Access Journals (Sweden)

Zhen Ding

2014-01-01

Full Text Available The pressing issue of earthy and musty odor compounds in natural waters, which can affect the organoleptic properties of drinking water, makes it a public health concern. A simple and sensitive method for simultaneous analysis of five odorants in environmental water was developed by headspace solid-phase microextraction (HS-SPME coupled to chromatography-mass spectrometry (GC-MS, including geosmin (GSM and 2-methylisoborneol (2-MIB, as well as dimethyl trisulfide (DMTS, β-cyclocitral, and β-ionone. Based on the simple modification of original magnetic stirrer purchased from CORNING (USA, the five target compounds can be separated within 23 min, and the calibration curves show good linearity with a correlation coefficient above 0.999 (levels = 5. The limits of detection (LOD are all below 1.3 ng L−1, and the relative standard deviation (%RSD is between 4.4% and 9.9% (n = 7 and recoveries of the analytes from water samples are between 86.2% and 112.3%. In addition, the storage time experiment indicated that the concentrations did not change significantly for GSM and 2-MIB if they were stored in canonical environment. In conclusion, the method in this study could be applied for monitoring these five odorants in natural waters.

Packaging and transportation of derived enriched uranium for the ''megatons to megawatts'' USA/Russia agreement

International Nuclear Information System (INIS)

Darrough, E.; Ewing, L.; Ravenscroft, N.

1998-01-01

In January 1998 the United States Enrichment Corporation (USEC) and Techsnabexport Co., Ltd (TENEX) of Russia celebrated the fourth anniversary of the signing of the 20-year contract between these two executive agents. USEC and TENEX are responsible for implementing the Government to-Government agreement between the United States and the Russian Federation for the purchase of uranium derived from dismantled nuclear weapons from the former Soviet Union. This program, entitled 'Megatons to Megawatts', is the first time nuclear warheads have been turned into fuel as well as the first time a commercial contract has been used to implement such a program. As of the fourth anniversary, the equivalent of almost 1,200 nuclear warheads had been converted to fuel. USEC is responsible for making all of the arrangements to transport the Russian LEU derived from HEU--hence the term, derived enriched uranium (DEU)--from St Petersburg. Russia to the USEC plant near portsmouth, Ohio. Edlow International Company is working with USEC to implement the shipping campaign and is responsible for coordination of the port delivery within Russia, as well. The organization responsible for these shipments within Russia is IZOTOP. While the program has been a major new responsibility for USEC, the early years of the program prepared all parties for the future challenges such as increased numbers of shipments, additional originating sites in Russia and witnessing requirements in Russia. (authors)

Plant-Derived Tick Repellents Activate the Honey Bee Ectoparasitic Mite TRPA1

Directory of Open Access Journals (Sweden)

Guangda Peng

2015-07-01

Full Text Available We have identified and characterized the TRPA1 channel of Varroa destructor (VdTRPA1, a major ectoparasitic mite of honey bee. One of the two VdTRPA1 isoforms, VdTRPA1L, was activated by a variety of plant-derived compounds, including electrophilic compounds, suggesting that chemical activation profiles are mostly shared between arthropod TRPA1 channels. Nevertheless, carvacrol and α-terpineol activated VdTRPA1L but not a honey bee noxious-stimuli-sensitive TRPA, AmHsTRPA, and Drosophila melanogaster TRPA1. Activation of VdTRPA1L in D. melanogaster taste neurons by the above compounds was sufficient to modify the gustatory behaviors. Carvacrol and α-terpineol repelled V. destructor in a laboratory assay, and α-terpineol repressed V. destructor entry for reproduction into the brood cells in hives. Understanding the functions of parasite TRP channels not only gives clues about the evolving molecular and cellular mechanisms of parasitism but also helps in the development of control methods.

Protein model discrimination using mutational sensitivity derived from deep sequencing.

Science.gov (United States)

Adkar, Bharat V; Tripathi, Arti; Sahoo, Anusmita; Bajaj, Kanika; Goswami, Devrishi; Chakrabarti, Purbani; Swarnkar, Mohit K; Gokhale, Rajesh S; Varadarajan, Raghavan

2012-02-08

A major bottleneck in protein structure prediction is the selection of correct models from a pool of decoys. Relative activities of ∼1,200 individual single-site mutants in a saturation library of the bacterial toxin CcdB were estimated by determining their relative populations using deep sequencing. This phenotypic information was used to define an empirical score for each residue (RankScore), which correlated with the residue depth, and identify active-site residues. Using these correlations, ∼98% of correct models of CcdB (RMSD ≤ 4Å) were identified from a large set of decoys. The model-discrimination methodology was further validated on eleven different monomeric proteins using simulated RankScore values. The methodology is also a rapid, accurate way to obtain relative activities of each mutant in a large pool and derive sequence-structure-function relationships without protein isolation or characterization. It can be applied to any system in which mutational effects can be monitored by a phenotypic readout. Copyright © 2012 Elsevier Ltd. All rights reserved.

Determination of benzene in different food matrices by distillation and isotope dilution HS-GC/MS

International Nuclear Information System (INIS)

Medeiros Vinci, Raquel; Canfyn, Michael; De Meulenaer, Bruno; Schaetzen, Thibault de; Van Overmeire, Ilse; De Beer, Jacques; Van Loco, Joris

2010-01-01

Benzene is classified by the IARC as carcinogenic to humans. Several sources may contribute for the occurrence of benzene in foods, such as, environmental contamination and the reaction of benzoate salts with ascorbic acid (naturally present or added as food additives). Matrix effect on benzene recovery (e.g. in fatty foods) and artefactual benzene formation from benzoate during analysis in the presence of ascorbate are some of the challenges presented when determining benzene in a wide range of foodstuffs. Design of experiment (DOE) was used to determine the most important variables in benzene recovery from headspace GC/MS. Based on the results of the DOE, a versatile method for the extraction of benzene from all kind of food commodities was developed. The method which consisted of distillation and isotope dilution HS-GC/MS was in-house validated. Artefactual benzene was prevented by addition of a borate buffer solution (pH 11) under distillation conditions. The method presented in this study allows the use of a matrix-independent calibration with detection limits below the legal limit established by the European Council for benzene in drinking water (1 μg L -1 ).

Determination of benzene in different food matrices by distillation and isotope dilution HS-GC/MS

Energy Technology Data Exchange (ETDEWEB)

Medeiros Vinci, Raquel [Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent (Belgium); Canfyn, Michael [Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Rue Juliette Wytsmanstraat 14, 1050 Brussels (Belgium); De Meulenaer, Bruno [Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent (Belgium); Schaetzen, Thibault de; Van Overmeire, Ilse; De Beer, Jacques [Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Rue Juliette Wytsmanstraat 14, 1050 Brussels (Belgium); Van Loco, Joris, E-mail: Joris.VanLoco@iph.fgov.BE [Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Rue Juliette Wytsmanstraat 14, 1050 Brussels (Belgium)

2010-07-05

Benzene is classified by the IARC as carcinogenic to humans. Several sources may contribute for the occurrence of benzene in foods, such as, environmental contamination and the reaction of benzoate salts with ascorbic acid (naturally present or added as food additives). Matrix effect on benzene recovery (e.g. in fatty foods) and artefactual benzene formation from benzoate during analysis in the presence of ascorbate are some of the challenges presented when determining benzene in a wide range of foodstuffs. Design of experiment (DOE) was used to determine the most important variables in benzene recovery from headspace GC/MS. Based on the results of the DOE, a versatile method for the extraction of benzene from all kind of food commodities was developed. The method which consisted of distillation and isotope dilution HS-GC/MS was in-house validated. Artefactual benzene was prevented by addition of a borate buffer solution (pH 11) under distillation conditions. The method presented in this study allows the use of a matrix-independent calibration with detection limits below the legal limit established by the European Council for benzene in drinking water (1 {mu}g L{sup -1}).

Determination of fatty acids and volatile compounds in fruits of rosehip(Rosa L.) species by HS-SPME/GC-MS and Im-SPME/GC-MS techniques

OpenAIRE

MURATHAN, ZEHRA TUĞBA; ZARIFIKHOSROSHAHI, MOZGAN; KAFKAS, NESİBE EBRU

2016-01-01

In this study, we aimed to compare fatty acid and volatile compound compositions of four rosehip species, namely Rosa pimpinellifolia, R. Villosa, R. Canina, and R. Dumalis, by gas chromatography with flame ionization detector (GC/FID) and headspace and immersion solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS and Im-SPME/GC-MS) techniques. The total lipid contents in fruits of the rosehip species varied from 5.83% (R. Villosa) to 7.84% (R. Dumalis). A total of...

Modified HS-SPME for determination of quantitative relations between low-molecular oxygen compounds in various matrices.

Science.gov (United States)

Dawidowicz, Andrzej L; Szewczyk, Joanna; Dybowski, Michal P

2016-09-07

Similar quantitative relations between individual constituents of the liquid sample established by its direct injection can be obtained applying Polydimethylsiloxane (PDMS) fiber in the headspace solid phase microextraction (HS-SPME) system containing the examined sample suspended in methyl silica oil. This paper proves that the analogous system composed of sample suspension/emulsion in polyethylene glycol (PEG) and Carbowax fiber allows to get similar quantitative relations between components of the mixture as those established by its direct analysis, but only for polar constituents. It is demonstrated for essential oil (EO) components of savory, sage, mint and thyme, and of artificial liquid mixture of polar constituents. The observed differences in quantitative relations between polar constituents estimated by both applied procedures are insignificant (Fexp < Fcrit). The presented results indicates that wider applicability of the system composed of a sample suspended in the oil of the same physicochemical character as that of used SPME fiber coating strongly depends on the character of interactions between analytes-suspending liquid and analytes-fiber coating. Copyright © 2016 Elsevier B.V. All rights reserved.

Liquid chromatography/tandem mass spectrometry method for quantitative estimation of solutol HS15 and its applications.

Science.gov (United States)

Bhaskar, V Vijaya; Middha, Anil; Srivastava, Pratima; Rajagopal, Sriram

2015-04-01

A rapid, sensitive and selective pseudoMRM (pMRM)-based method for the determination of solutol HS15 (SHS15) in rat plasma was developed using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The most abundant ions corresponding to SHS15 free polyethyleneglycol (PEG) oligomers at m / z 481, 525, 569, 613, 657, 701, 745, 789, 833, 877, 921 and 965 were selected for pMRM in electrospray mode of ionization. Purity of the lipophilic and hydrophilic components of SHS15 was estimated using evaporative light scattering detector (ELSD). Plasma concentrations of SHS15 were measured after oral administration at 2.50 g/kg dose and intravenous administration at 1.00 g/kg dose in male Sprague Dawley rats. SHS15 has poor oral bioavailability of 13.74% in rats. Differences in pharmacokinetics of oligomers were studied. A novel proposal was conveyed to the scientific community, where formulation excipient could be analyzed as a qualifier in the analysis of new chemical entities (NCEs) to address the spiky plasma concentration profiles.

Multiple internal standard normalization for improving HS-SPME-GC-MS quantitation in virgin olive oil volatile organic compounds (VOO-VOCs) profile.

Science.gov (United States)

Fortini, Martina; Migliorini, Marzia; Cherubini, Chiara; Cecchi, Lorenzo; Calamai, Luca

2017-04-01

The commercial value of virgin olive oils (VOOs) strongly depends on their classification, also based on the aroma of the oils, usually evaluated by a panel test. Nowadays, a reliable analytical method is still needed to evaluate the volatile organic compounds (VOCs) and support the standard panel test method. To date, the use of HS-SPME sampling coupled to GC-MS is generally accepted for the analysis of VOCs in VOOs. However, VOO is a challenging matrix due to the simultaneous presence of: i) compounds at ppm and ppb concentrations; ii) molecules belonging to different chemical classes and iii) analytes with a wide range of molecular mass. Therefore, HS-SPME-GC-MS quantitation based upon the use of external standard method or of only a single internal standard (ISTD) for data normalization in an internal standard method, may be troublesome. In this work a multiple internal standard normalization is proposed to overcome these problems and improving quantitation of VOO-VOCs. As many as 11 ISTDs were used for quantitation of 71 VOCs. For each of them the most suitable ISTD was selected and a good linearity in a wide range of calibration was obtained. Except for E-2-hexenal, without ISTD or with an unsuitable ISTD, the linear range of calibration was narrower with respect to that obtained by a suitable ISTD, confirming the usefulness of multiple internal standard normalization for the correct quantitation of VOCs profile in VOOs. The method was validated for 71 VOCs, and then applied to a series of lampante virgin olive oils and extra virgin olive oils. In light of our results, we propose the application of this analytical approach for routine quantitative analyses and to support sensorial analysis for the evaluation of positive and negative VOOs attributes. Copyright © 2017 Elsevier B.V. All rights reserved.

Hurricane Imaging Radiometer (HIRAD) Observations of Brightness Temperatures and Ocean Surface Wind Speed and Rain Rate During NASA's GRIP and HS3 Campaigns

Science.gov (United States)

Miller, Timothy L.; James, M. W.; Roberts, J. B.; Jones, W. L.; Biswas, S.; Ruf, C. S.; Uhlhorn, E. W.; Atlas, R.; Black, P.; Albers, C.

2012-01-01

HIRAD flew on high-altitude aircraft over Earl and Karl during NASA s GRIP (Genesis and Rapid Intensification Processes) campaign in August - September of 2010, and plans to fly over Atlantic tropical cyclones in September of 2012 as part of the Hurricane and Severe Storm Sentinel (HS3) mission. HIRAD is a new C-band radiometer using a synthetic thinned array radiometer (STAR) technology to obtain spatial resolution of approximately 2 km, out to roughly 30 km each side of nadir. By obtaining measurements of emissions at 4, 5, 6, and 6.6 GHz, observations of ocean surface wind speed and rain rate can be retrieved. The physical retrieval technique has been used for many years by precursor instruments, including the Stepped Frequency Microwave Radiometer (SFMR), which has been flying on the NOAA and USAF hurricane reconnaissance aircraft for several years to obtain observations within a single footprint at nadir angle. Results from the flights during the GRIP and HS3 campaigns will be shown, including images of brightness temperatures, wind speed, and rain rate. Comparisons will be made with observations from other instruments on the campaigns, for which HIRAD observations are either directly comparable or are complementary. Features such as storm eye and eye-wall, location of storm wind and rain maxima, and indications of dynamical features such as the merging of a weaker outer wind/rain maximum with the main vortex may be seen in the data. Potential impacts on operational ocean surface wind analyses and on numerical weather forecasts will also be discussed.

Application of Hybrid HS and Tabu Search Algorithm for Optimal Location of FACTS Devices to Reduce Power Losses in Power Systems

Directory of Open Access Journals (Sweden)

Z. Masomi Zohrabad

2016-12-01

Full Text Available Power networks continue to grow following the annual growth of energy demand. As constructing new energy generation facilities bears a high cost, minimizing power grid losses becomes essential to permit low cost energy transmission in larger distances and additional areas. This study aims to model an optimization problem for an IEEE 30-bus power grid using a Tabu search algorithm based on an improved hybrid Harmony Search (HS method to reduce overall grid losses. The proposed algorithm is applied to find the best location for the installation of a Unified Power Flow Controller (UPFC. The results obtained from installation of the UPFC in the grid are presented by displaying outputs.

Notch Sensitivity of Fatigue Behavior of a Hi-Nicalon™/SiC-B4C Composite at 1,200 °C in Air and in Steam

Science.gov (United States)

Ruggles-Wrenn, M. B.; Kurtz, G.

2013-10-01

The effect of holes on the fatigue life of a non-oxide ceramic composite processed via chemical vapor infiltration (CVI) was examined at 1,200 °C in laboratory air and in steam. The effect of holes on tensile strength at 1,200 °C was also evaluated. The composite comprised laminated woven Hi-Nicalon™ fibers in an oxidation inhibited matrix, which consisted of alternating layers of silicon carbide and boron carbide. Fiber preforms had pyrolytic carbon fiber coating with boron carbon overlay applied. Unnotched specimens and specimens with a center hole having a radius to width ratio of 0.24 were tested in tension-tension fatigue at 0.1 Hz and at 1.0 Hz. The fatigue stresses ranged from 100 to 140 MPa in air and in steam. Fatigue run-out was defined as 105 cycles at 0.1 Hz and as 2 × 105 cycles at 1.0 Hz. The net-section strength was less than the unnotched ultimate tensile strength. Comparison of notched and unnotched data also revealed that the fatigue performance was notch insensitive in both air and steam environments. Composite microstructure, as well as damage and failure mechanisms were investigated.

Effect of external pH on the cytoplasmic and vacuolar pHs in Mung bean root-tip cells

International Nuclear Information System (INIS)

Torimitsu, Keiichi; Yazaki, Yoshiaki; Nagasuka, Kinuyo; Ohta, Eiji; Sakata, Makoto

1984-01-01

The effect of the external pH on the intracellular pH in mung bean (Vigna mungo (L.) Hepper) root-tip cells was investigated with the 31 P nuclear magnetic resonance (NMR) method. The 31 P NMR spectra showed three peaks caused by cytoplasmic G-6-P, cytoplasmic Psub(i) and vacuolar Psub(i). The cytoplasmic and vacuolar pHs could be determined by comparing the Psub(i) chemical shifts with the titration curve. When the external pH was changed over a range from pH 3 to 10, the cytoplasmic pH showed smaller changes than the vacuolar pH, suggesting that the former is regulated more strictly than the latter. The H + -ATPase inhibitor, DCCD, caused the breakdown of the mechanism that regulates the intracellular pH. H + -ATPase appears to have an important part in the regulation of the intracellular pH. (author)

Sistema de secado combinado para cerámica mediante tecnología microondas para el sector de la cerámica hasta formato 1200

Directory of Open Access Journals (Sweden)

TECNOLOGIA,SL, BULMA

2010-04-01

Full Text Available New team of passage by technology microwaves for dried laminar of materials of high absorption, and dried green porcelain format until 1200 mm and thicknesses until 25 mm. It allows the elimination of water with improvement of productivity, energetic efficiency and clean technology for processed of marble, natural stone, ceramic tiles, plasters and products in bulk, without broadcasts of CO₂, and respectful with the Nature, allows the dried with low degree. Project manufactured and designed totally by BULMA situated in L’Alcudia protected with national patent number P200931081.

Nuevo equipo de pasaje por tecnología microondas para secado laminar de materiales de alta absorción,y secado de bizcocho en verde formato hasta 1200 mm y espesores hasta 25 mm. Permite la eliminación de humedad con mejora de productividad, eficiencia energética y tecnológicamente limpios para procesado de mármol, piedra natural, cerámica, escayola y productos a granel, sin emisiones de CO₂, y respetuoso con el medio ambiente, permite el secado con bajos gradientes, fabricado y diseñado íntegramente por empresa valenciana BULMA situada en L’Alcudia protegido con patente nacional P200931081 y en tramite internacional.