Cell-type-specific predictive network yields novel insights into mouse embryonic stem cell self-renewal and cell fate.

Directory of Open Access Journals (Sweden)

Karen G Dowell

Full Text Available Self-renewal, the ability of a stem cell to divide repeatedly while maintaining an undifferentiated state, is a defining characteristic of all stem cells. Here, we clarify the molecular foundations of mouse embryonic stem cell (mESC self-renewal by applying a proven Bayesian network machine learning approach to integrate high-throughput data for protein function discovery. By focusing on a single stem-cell system, at a specific developmental stage, within the context of well-defined biological processes known to be active in that cell type, we produce a consensus predictive network that reflects biological reality more closely than those made by prior efforts using more generalized, context-independent methods. In addition, we show how machine learning efforts may be misled if the tissue specific role of mammalian proteins is not defined in the training set and circumscribed in the evidential data. For this study, we assembled an extensive compendium of mESC data: ∼2.2 million data points, collected from 60 different studies, under 992 conditions. We then integrated these data into a consensus mESC functional relationship network focused on biological processes associated with embryonic stem cell self-renewal and cell fate determination. Computational evaluations, literature validation, and analyses of predicted functional linkages show that our results are highly accurate and biologically relevant. Our mESC network predicts many novel players involved in self-renewal and serves as the foundation for future pluripotent stem cell studies. This network can be used by stem cell researchers (at http://StemSight.org to explore hypotheses about gene function in the context of self-renewal and to prioritize genes of interest for experimental validation.

Muscle satellite cell heterogeneity and self-renewal

Science.gov (United States)

Motohashi, Norio; Asakura, Atsushi

2014-01-01

Adult skeletal muscle possesses extraordinary regeneration capacities. After muscle injury or exercise, large numbers of newly formed muscle fibers are generated within a week as a result of expansion and differentiation of a self-renewing pool of muscle stem cells termed muscle satellite cells. Normally, satellite cells are mitotically quiescent and reside beneath the basal lamina of muscle fibers. Upon regeneration, satellite cells are activated, and give rise to daughter myogenic precursor cells. After several rounds of proliferation, these myogenic precursor cells contribute to the formation of new muscle fibers. During cell division, a minor population of myogenic precursor cells returns to quiescent satellite cells as a self-renewal process. Currently, accumulating evidence has revealed the essential roles of satellite cells in muscle regeneration and the regulatory mechanisms, while it still remains to be elucidated how satellite cell self-renewal is molecularly regulated and how satellite cells are important in aging and diseased muscle. The number of satellite cells is decreased due to the changing niche during ageing, resulting in attenuation of muscle regeneration capacity. Additionally, in Duchenne muscular dystrophy (DMD) patients, the loss of satellite cell regenerative capacity and decreased satellite cell number due to continuous needs for satellite cells lead to progressive muscle weakness with chronic degeneration. Thus, it is necessary to replenish muscle satellite cells continuously. This review outlines recent findings regarding satellite cell heterogeneity, asymmetric division and molecular mechanisms in satellite cell self-renewal which is crucial for maintenance of satellite cells as a muscle stem cell pool throughout life. In addition, we discuss roles in the stem cell niche for satellite cell maintenance, as well as related cell therapies for approaching treatment of DMD. PMID:25364710

Muscle Satellite Cell Heterogeneity and Self-Renewal

Directory of Open Access Journals (Sweden)

Norio eMotohashi

2014-01-01

Full Text Available Adult skeletal muscle possesses extraordinary regeneration capacities. After muscle injury or exercise, large numbers of newly formed muscle fibers are generated within a week as a result of expansion and differentiation of a self-renewing pool of muscle stem cells termed muscle satellite cells. Normally, satellite cells are mitotically quiescent and reside beneath the basal lamina of muscle fibers. Upon regeneration, satellite cells are activated, and give rise to daughter myogenic precursor cells. After several rounds of proliferation, these myogenic precursor cells contribute to the formation of new muscle fibers. During cell division, a minor population of myogenic precursor cells returns to quiescent satellite cells as a self-renewal process. Currently, accumulating evidence has revealed the essential roles of satellite cells in muscle regeneration and the regulatory mechanisms, while it still remains to be elucidated how satellite cell self-renewal is molecularly regulated and how satellite cells are important in aging and diseased muscle. The number of satellite cells is decreased due to the changing niche during ageing, resulting in attenuation of muscle regeneration capacity. Additionally, in Duchenne muscular dystrophy (DMD patients, the loss of satellite cell regenerative capacity and decreased satellite cell number due to continuous needs for satellite cells lead to progressive muscle weakness with chronic degeneration. Thus, it is necessary to replenish muscle satellite cells continuously. This review outlines recent findings regarding satellite cell heterogeneity, asymmetric division and molecular mechanisms in satellite cell self-renewal which is crucial for maintenance of satellite cells as a muscle stem cell pool throughout life. In addition, we discuss roles in the stem cell niche for satellite cell maintenance, as well as related cell therapies for approaching treatment of DMD.

Renewable energy systems a smart energy systems approach to the choice and modeling of 100% renewable solutions

CERN Document Server

Lund, Henrik

2014-01-01

In this new edition of Renewable Energy Systems, globally recognized renewable energy researcher and professor, Henrik Lund, sets forth a straightforward, comprehensive methodology for comparing different energy systems' abilities to integrate fluctuating and intermittent renewable energy sources. The book does this by presenting an energy system analysis methodology and offering a freely available accompanying software tool, EnergyPLAN, which automates and simplifies the calculations supporting such a detailed comparative analysis. The book provides the results of more than fifteen comprehensive energy system analysis studies, examines the large-scale integration of renewable energy into the present system, and presents concrete design examples derived from a dozen renewable energy systems around the globe. Renewable Energy Systems, Second Edition also undertakes the socio-political realities governing the implementation of renewable energy systems by introducing a theoretical framework approach aimed at ...

Response of Non-Linear Systems to Renewal Impulses by Path Integration

DEFF Research Database (Denmark)

Nielsen, Søren R.K.; Iwankiewicz, R.

The cell-to-cell mapping (path integration) technique has been devised for MDOF non-linear and non-hysteretic systems subjected to random trains of impulses driven by an ordinary renewal point process with gamma-distributed integer parameter interarrival times (an Erlang process). Since the renewal...... point process has not independent increments the state vector of the system, consisting of the generalized displacements and velocities, is not a Markov process. Initially it is shown how the indicated systems can be converted to an equivalent Poisson driven system at the expense of introducing...... additional discrete-valued state variables for which the stochastic equations are also formulated....

Adhesion-mediated self-renewal abilities of Ph+ blastoma cells

International Nuclear Information System (INIS)

Funayama, Keiji; Saito-Kurimoto, Yumi; Ebihara, Yasuhiro; Shimane, Miyuki; Nomura, Hitoshi; Tsuji, Ko-ichiro; Asano, Shigetaka

2010-01-01

The Philadelphia chromosome-positive blastoma, maintained by serial subcutaneous transplantation in nude mice, is a highly proliferating biological mass consisting of homogenous CD34 + CD38 - myeloblastoid cells. These cells newly evolved from pluripotent leukemia stem cells of chronic myeloid leukemia in the chronic phase. Therefore, this mass may provide a unique tool for better understanding cellular and molecular mechanisms of self-renewal of leukemia stem cells. In this paper, we demonstrated that intravenously injected blastoma cells can cause Ph+ blastic leukemia with multiple invasive foci in NOD/SCID mice but not in nude mice. In addition, using an in vitro culture system, we clearly showed that blastoma cell adhesion to OP9 stromal cells accelerates blastoma cell proliferation that is associated with up-regulation of BMI1 gene expression; increased levels of β-catenin and the Notch1 intra-cellular domain; and changed the expression pattern of variant CD44 forms, which are constitutively expressed in these blastoma cells. These findings strongly suggest that adhesion of leukemic stem cells to stromal cells via CD44 might be indispensable for their cellular defense against attack by immune cells and for maintenance of their self-renewal ability.

Design, Operation, Control, and Economics of a Photovoltaic/Fuel Cell/Battery Hybrid Renewable Energy System for Automotive Applications

Directory of Open Access Journals (Sweden)

Zachary S. Whiteman

2015-06-01

Full Text Available Meeting rapidly growing global energy demand—without producing greenhouse gases or further diminishing the availability of non-renewable resources—requires the development of affordable low-emission renewable energy systems. Here, we develop a hybrid renewable energy system (HRES for automotive applications—specifically, a roof-installed photovoltaic (PV array combined with a PEM fuel cell/NiCd battery bus currently operating shuttle routes on the University of Delaware campus. The system’s overall operating objectives—meeting the total power demand of the bus and maintaining the desired state of charge (SOC of the NiCd battery—are achieved with appropriately designed controllers: a logic-based “algebraic controller” and a standard PI controller. The design, implementation, and performance of the hybrid system are demonstrated via simulation of real shuttle runs under various operating conditions. The results show that both control strategies perform equally well in enabling the HRES to meet its objectives under typical operating conditions, and under sudden cloud cover conditions; however, at consistently high bus speeds, battery SOC maintenance is better, and the system consumes less hydrogen, with PI control. An economic analysis of the PV investment necessary to realize the HRES design objectives indicates a return on investment of approximately 30% (a slight, but nonetheless positive, ~$550 profit over the bus lifetime in Newark, DE, establishing the economic viability of the proposed addition of a PV array to the existing University of Delaware fuel cell/battery bus.

Fuel cells as renewable energy sources

International Nuclear Information System (INIS)

Cacciola, G.; Passalacqua, E.

2001-01-01

The technology level achieved in fuel cell (FC) systems in the last years has significantly increased the interest of various manufacturing industries engaged in energy production and distribution even under the perspectives that this technology could provide. Today, the fuel cells (FCs) can supply both electrical and thermal energy without using moving parts and with a high level of affordability with respect to the conventional systems. FCs can utilise every kind of fuel such as hydrocarbons, hydrogen available from the water through renewable sources (wind, solar energy), alcohol etc. Thus, they may find application in many field ranging from energy production in large or small plants to the cogeneration systems for specific needs such as for residential applications, hospitals, industries, electric vehicles and portable power sources. Low temperature polymer electrolyte fuel cells (PEFC, DMFC) are preferred for application in the field of transportation and portable systems. The CNR-ITAE research activity in this field concerns the development of technologies, materials and components for the entire system: electrocatalysts, conducting supports, electrolytes, manufacturing technologies for the electrodes-electrolyte assemblies and the attainment of fuel cells with high power densities. Furthermore, some activities have been devoted to the design and realisation of PEFC fuel cell prototypes with rated power lower than I kW for stationary and mobile applications [it

Adaptive control paradigm for photovoltaic and solid oxide fuel cell in a grid-integrated hybrid renewable energy system.

Science.gov (United States)

Mumtaz, Sidra; Khan, Laiq

2017-01-01

The hybrid power system (HPS) is an emerging power generation scheme due to the plentiful availability of renewable energy sources. Renewable energy sources are characterized as highly intermittent in nature due to meteorological conditions, while the domestic load also behaves in a quite uncertain manner. In this scenario, to maintain the balance between generation and load, the development of an intelligent and adaptive control algorithm has preoccupied power engineers and researchers. This paper proposes a Hermite wavelet embedded NeuroFuzzy indirect adaptive MPPT (maximum power point tracking) control of photovoltaic (PV) systems to extract maximum power and a Hermite wavelet incorporated NeuroFuzzy indirect adaptive control of Solid Oxide Fuel Cells (SOFC) to obtain a swift response in a grid-connected hybrid power system. A comprehensive simulation testbed for a grid-connected hybrid power system (wind turbine, PV cells, SOFC, electrolyzer, battery storage system, supercapacitor (SC), micro-turbine (MT) and domestic load) is developed in Matlab/Simulink. The robustness and superiority of the proposed indirect adaptive control paradigm are evaluated through simulation results in a grid-connected hybrid power system testbed by comparison with a conventional PI (proportional and integral) control system. The simulation results verify the effectiveness of the proposed control paradigm.

Adaptive control paradigm for photovoltaic and solid oxide fuel cell in a grid-integrated hybrid renewable energy system

Science.gov (United States)

Khan, Laiq

2017-01-01

The hybrid power system (HPS) is an emerging power generation scheme due to the plentiful availability of renewable energy sources. Renewable energy sources are characterized as highly intermittent in nature due to meteorological conditions, while the domestic load also behaves in a quite uncertain manner. In this scenario, to maintain the balance between generation and load, the development of an intelligent and adaptive control algorithm has preoccupied power engineers and researchers. This paper proposes a Hermite wavelet embedded NeuroFuzzy indirect adaptive MPPT (maximum power point tracking) control of photovoltaic (PV) systems to extract maximum power and a Hermite wavelet incorporated NeuroFuzzy indirect adaptive control of Solid Oxide Fuel Cells (SOFC) to obtain a swift response in a grid-connected hybrid power system. A comprehensive simulation testbed for a grid-connected hybrid power system (wind turbine, PV cells, SOFC, electrolyzer, battery storage system, supercapacitor (SC), micro-turbine (MT) and domestic load) is developed in Matlab/Simulink. The robustness and superiority of the proposed indirect adaptive control paradigm are evaluated through simulation results in a grid-connected hybrid power system testbed by comparison with a conventional PI (proportional and integral) control system. The simulation results verify the effectiveness of the proposed control paradigm. PMID:28329015

Haemopoietic cell renewal in radiation fields

Science.gov (United States)

Fliedner, T. M.; Nothdurft, W.; Tibken, B.; Hofer, E.; Weiss, M.; Kindler, H.

1994-10-01

Space flight activities are inevitably associated with a chronic exposure of astronauts to a complex mixture of ionising radiation. Although no acute radiation consequences are to be expected as a rule, the possibility of Solar Particle Events (SPE) associated with relatively high doses of radiation (1 or more Gray) cannot be excluded. It is the responsibility of physicians in charge of the health of astronauts to evaluate before, during and after space flight activities the functional status of haemopoietic cell renewal. Chronic low level exposure of dogs indicate that daily gamma-exposure doses below about 2 cGy are tolerated for several years as far as blood cell concentrations are concerned. However, the stem cell pool may be severely affected. The maintenance of sufficient blood cell counts is possible only through increased cell production to compensate for the radiation inflicted excess cell loss. This behaviour of haemopoietic cell renewal during chronic low level exposure can be simulated by bioengineering models of granulocytopoiesis. It is possible to define a ``turbulence region'' for cell loss rates, below which an prolonged adaptation to increased radiation fields can be expected to be tolerated. On the basis of these experimental results, it is recommended to develop new biological indicators to monitor haemopoietic cell renewal at the level of the stem cell pool using blood stem cells in addition to the determination of cytokine concentrations in the serum (and other novel approaches). To prepare for unexpected haemopoietic effects during prolonged space missions, research should be increased to modify the radiation sensitivity of haemopoietic stem cells (for instance by the application of certain regulatory molecules). In addition, a ``blood stem cell bank'' might be established for the autologous storage of stem cells and for use in space activities keeping them in a radiation protected container.

Porcine spermatogonial stem cells self-renew effectively in a three dimensional culture microenvironment.

Science.gov (United States)

Park, Ji Eun; Park, Min Hee; Kim, Min Seong; Park, Yeo Reum; Yun, Jung Im; Cheong, Hee Tae; Kim, Minseok; Choi, Jung Hoon; Lee, Eunsong; Lee, Seung Tae

2017-12-01

Generally, self-renewal of spermatogonial stem cells (SSCs) is maintained in vivo in a three-dimensional (3D) microenvironment consisting of the seminiferous tubule basement membrane, indicating the importance of the 3D microenvironment for in vitro culture of SSCs. Here, we report a 3D culture microenvironment that effectively maintains porcine SSC self-renewal during culture. Porcine SSCs were cultured in an agarose-based 3D hydrogel and in 2D culture plates either with or without feeder cells. Subsequently, the effects of 3D culture on the maintenance of undifferentiated SSCs were identified by analyzing cell colony formation and morphology, AP activity, and transcriptional and translational regulation of self-renewal-related genes and the effects on proliferation by analyzing cell viability and single cell-derived colony number. The 3D culture microenvironment constructed using a 0.2% (w/v) agarose-based 3D hydrogel showed the strongest maintenance of porcine SSC self-renewal and induced significant improvements in proliferation compared with 2D culture microenvironments. These results demonstrate that self-renewal of porcine SSCs can be maintained more effectively in a 3D than in a 2D culture microenvironment. Moreover, this will play a significant role in developing novel culture systems for SSCs derived from diverse species in the future, which will contribute to SSC-related research. © 2017 International Federation for Cell Biology.

Uhrf1 controls the self-renewal versus differentiation of hematopoietic stem cells by epigenetically regulating the cell-division modes.

Science.gov (United States)

Zhao, Jingyao; Chen, Xufeng; Song, Guangrong; Zhang, Jiali; Liu, Haifeng; Liu, Xiaolong

2017-01-10

Hematopoietic stem cells (HSCs) are able to both self-renew and differentiate. However, how individual HSC makes the decision between self-renewal and differentiation remains largely unknown. Here we report that ablation of the key epigenetic regulator Uhrf1 in the hematopoietic system depletes the HSC pool, leading to hematopoietic failure and lethality. Uhrf1-deficient HSCs display normal survival and proliferation, yet undergo erythroid-biased differentiation at the expense of self-renewal capacity. Notably, Uhrf1 is required for the establishment of DNA methylation patterns of erythroid-specific genes during HSC division. The expression of these genes is enhanced in the absence of Uhrf1, which disrupts the HSC-division modes by promoting the symmetric differentiation and suppressing the symmetric self-renewal. Moreover, overexpression of one of the up-regulated genes, Gata1, in HSCs is sufficient to phenocopy Uhrf1-deficient HSCs, which show impaired HSC symmetric self-renewal and increased differentiation commitment. Taken together, our findings suggest that Uhrf1 controls the self-renewal versus differentiation of HSC through epigenetically regulating the cell-division modes, thus providing unique insights into the relationship among Uhrf1-mediated DNA methylation, cell-division mode, and HSC fate decision.

Novel insights into embryonic stem cell self-renewal revealed through comparative human and mouse systems biology networks.

Science.gov (United States)

Dowell, Karen G; Simons, Allen K; Bai, Hao; Kell, Braden; Wang, Zack Z; Yun, Kyuson; Hibbs, Matthew A

2014-05-01

Embryonic stem cells (ESCs), characterized by their ability to both self-renew and differentiate into multiple cell lineages, are a powerful model for biomedical research and developmental biology. Human and mouse ESCs share many features, yet have distinctive aspects, including fundamental differences in the signaling pathways and cell cycle controls that support self-renewal. Here, we explore the molecular basis of human ESC self-renewal using Bayesian network machine learning to integrate cell-type-specific, high-throughput data for gene function discovery. We integrated high-throughput ESC data from 83 human studies (~1.8 million data points collected under 1,100 conditions) and 62 mouse studies (~2.4 million data points collected under 1,085 conditions) into separate human and mouse predictive networks focused on ESC self-renewal to analyze shared and distinct functional relationships among protein-coding gene orthologs. Computational evaluations show that these networks are highly accurate, literature validation confirms their biological relevance, and reverse transcriptase polymerase chain reaction (RT-PCR) validation supports our predictions. Our results reflect the importance of key regulatory genes known to be strongly associated with self-renewal and pluripotency in both species (e.g., POU5F1, SOX2, and NANOG), identify metabolic differences between species (e.g., threonine metabolism), clarify differences between human and mouse ESC developmental signaling pathways (e.g., leukemia inhibitory factor (LIF)-activated JAK/STAT in mouse; NODAL/ACTIVIN-A-activated fibroblast growth factor in human), and reveal many novel genes and pathways predicted to be functionally associated with self-renewal in each species. These interactive networks are available online at www.StemSight.org for stem cell researchers to develop new hypotheses, discover potential mechanisms involving sparsely annotated genes, and prioritize genes of interest for experimental validation

ROS Are Required for Mouse Spermatogonial Stem Cell Self-Renewal

OpenAIRE

Morimoto, Hiroko; Iwata, Kazumi; Ogonuki, Narumi; Inoue, Kimiko; Ogura, Atsuo; Kanatsu-Shinohara, Mito; Morimoto, Takeshi; Yabe-Nishimura, Chihiro; Shinohara, Takashi

2013-01-01

Reactive oxygen species (ROS) generation is implicated in stem cell self-renewal in several tissues but is thought to be detrimental for spermatogenesis as well as spermatogonial stem cells (SSCs). Using cultured SSCs, we show that ROS are generated via the AKT and MEK signaling pathways under conditions where the growth factors glial cell line-derived neurotrophic factor and fibroblast growth factor 2 drive SSC self-renewal and, instead, stimulate self-renewal at physiological levels. SSCs d...

Power electronics for renewable and distributed energy systems a sourcebook of topologies, control and integration

CERN Document Server

Chakraborty, Sudipta; Kramer, William E

2013-01-01

While most books approach power electronics and renewable energy as two separate subjects, Power Electronics for Renewable and Distributed Energy Systems takes an integrative approach; discussing power electronic converters topologies, controls and integration that are specific to the renewable and distributed energy system applications. An overview of power electronic technologies is followed by the introduction of various renewable and distributed energy resources that includes photovoltaics, wind, small hydroelectric, fuel cells, microturbines and variable speed generation. Energy storage s

Renewable Energy Systems

DEFF Research Database (Denmark)

Lund, Henrik; Mathiesen, Brian Vad; Connolly, David

2014-01-01

on the electricity sector, smart energy systems include the entire energy system in its approach to identifying suitable energy infrastructure designs and operation strategies. The typical smart grid sole focus on the electricity sector often leads to the conclusion that transmission lines, flexible electricity......This paper presents the learning of a series of studies that analyse the problems and perspectives of converting the present energy system into a 100 % renewable energy system using a smart energy systems approach. As opposed to, for instance, the smart grid concept, which takes a sole focus...... are to be found when the electricity sector is combined with the heating and cooling sectors and/or the transportation sector. Moreover, the combination of electricity and gas infrastructures may play an important role in the design of future renewable energy systems. The paper illustrates why electricity smart...

Renewable and efficient electric power systems

CERN Document Server

Masters, Gilbert M

2013-01-01

A solid, quantitative, practical introduction to a wide range of renewable energy systems-in a completely updated, new edition The second edition of Renewable and Efficient Electric Power Systems provides a solid, quantitative, practical introduction to a wide range of renewable energy systems. For each topic, essential theoretical background is introduced, practical engineering considerations associated with designing systems and predicting their performance are provided, and methods for evaluating the economics of these systems are presented. While the book focuses on

Metabolic rate determines haematopoietic stem cell self-renewal.

Science.gov (United States)

Sastry, P S R K

2004-01-01

The number of haematopoietic stem cells (HSCs) per animal is conserved across species. This means the HSCs need to maintain hematopoiesis over a longer period in larger animals. This would result in the requirement of stem cell self-renewal. At present the three existing models are the stochastic model, instructive model and the third more recently proposed is the chiaro-scuro model. It is a well known allometric law that metabolic rate scales to the three quarter power. Larger animals have a lower metabolic rate, compared to smaller animals. Here it is being hypothesized that metabolic rate determines haematopoietic stem cell self-renewal. At lower metabolic rate the stem cells commit for self-renewal, where as at higher metabolic rate they become committed to different lineages. The present hypothesis can explain the salient features of the different models. Recent findings regarding stem cell self-renewal suggest an important role for Wnt proteins and their receptors known as frizzleds, which are an important component of cell signaling pathway. The role of cGMP in the Wnts action provides further justification for the present hypothesis as cGMP is intricately linked to metabolic rate. One can also explain the telomere homeostasis by the present hypothesis. One prediction of the present hypothesis is with reference to the limit of cell divisions known as Hayflick limit, here it is being suggested that this is the result of metabolic rate in laboratory conditions and there can be higher number of cell divisions in vivo if the metabolic rate is lower. Copyright 2004 Elsevier Ltd.

Myostatin signals through Pax7 to regulate satellite cell self-renewal

International Nuclear Information System (INIS)

McFarlane, Craig; Hennebry, Alex; Thomas, Mark; Plummer, Erin; Ling, Nicholas; Sharma, Mridula; Kambadur, Ravi

2008-01-01

Myostatin, a Transforming Growth Factor-beta (TGF-β) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-dependent mechanism to regulate satellite cell self-renewal. While excess Myostatin inhibited Pax7 expression via ERK1/2 signaling, an increase in Pax7 expression was observed following both genetic inactivation and functional antagonism of Myostatin. As a result, we show that either blocking or inactivating Myostatin enhances the partitioning of the fusion-incompetent self-renewed satellite cell lineage (high Pax7 expression, low MyoD expression) from the pool of actively proliferating myogenic precursor cells. Consistent with this result, over-expression of Pax7 in C2C12 myogenic cells resulted in increased self-renewal through a mechanism which slowed both myogenic proliferation and differentiation. Taken together, these results suggest that increased expression of Pax7 promotes satellite cell self-renewal, and furthermore Myostatin may control the process of satellite cell self-renewal through regulation of Pax7. Thus we speculate that, in addition to the intrinsic factors (such as Pax7), extrinsic factors both positive and negative in nature, will play a major role in determining the stemness of skeletal muscle satellite cells

Potential of renewable energy systems in China

DEFF Research Database (Denmark)

Liu, Wen; Lund, Henrik; Mathiesen, Brian Vad

2011-01-01

Along with high-speed economic development and increasing energy consumption, the Chinese Government faces a growing pressure to maintain the balance between energy supply and demand. In 2009, China has become both the largest energy consumer and CO2 emitting country in the world. In this case...... system has demonstrated the possibility of converting into a 100% renewable energy system. This paper discusses the perspective of renewable energy in China firstly, and then analyses whether it is suitable to adopt similar methodologies applied in other countries as China approaches a renewable energy...... system. The conclusion is that China’s domestic renewable energy sources are abundant and show the possibility to cover future energy demand; the methodologies used to analyse a 100% renewable energy system are applicable in China. Therefore, proposing an analysis of a 100% renewable energy system...

Potential of renewable energy systems in China

International Nuclear Information System (INIS)

Liu, Wen; Lund, Henrik; Mathiesen, Brian Vad; Zhang, Xiliang

2011-01-01

Along with high-speed economic development and increasing energy consumption, the Chinese Government faces a growing pressure to maintain the balance between energy supply and demand. In 2009, China has become both the largest energy consumer and CO 2 emitting country in the world. In this case, the inappropriate energy consumption structure should be changed. As an alternative, a suitable infrastructure for the implementation of renewable energy may serve as a long-term sustainable solution. The perspective of a 100% renewable energy system has been analyzed and discussed in some countries previously. In this process, assessment of domestic renewable energy sources is the first step. Then appropriate methodologies are needed to perform energy system analyses involving the integration of more sustainable strategies. Denmark may serve as an example of how sustainable strategies can be implemented. The Danish system has demonstrated the possibility of converting into a 100% renewable energy system. This paper discusses the perspective of renewable energy in China firstly, and then analyses whether it is suitable to adopt similar methodologies applied in other countries as China approaches a renewable energy system. The conclusion is that China's domestic renewable energy sources are abundant and show the possibility to cover future energy demand; the methodologies used to analyse a 100% renewable energy system are applicable in China. Therefore, proposing an analysis of a 100% renewable energy system in China is not unreasonable. (author)

Renewable energy delivery systems and methods

Science.gov (United States)

Walker, Howard Andrew

2013-12-10

A system, method and/or apparatus for the delivery of energy at a site, at least a portion of the energy being delivered by at least one or more of a plurality of renewable energy technologies, the system and method including calculating the load required by the site for the period; calculating the amount of renewable energy for the period, including obtaining a capacity and a percentage of the period for the renewable energy to be delivered; comparing the total load to the renewable energy available; and, implementing one or both of additional and alternative renewable energy sources for delivery of energy to the site.

Understanding renewable energy systems

Energy Technology Data Exchange (ETDEWEB)

Quaschning, Volker

2005-01-15

Beginning with an overview of renewable energy sources including biomass, hydroelectricity, geothermal, tidal, wind and solar power, this book explores the fundamentals of different renewable energy systems. The main focus is on technologies with high development potential such as solar thermal systems, photovoltaics and wind power. This text not only describes technological aspects, but also deals consciously with problems of the energy industry. In this way, the topics are treated in a holistic manner, bringing together maths, engineering, climate studies and economics, and enabling readers to gain a broad understanding of renewable energy technologies and their potential. The book also contains a free CD-ROM resource, which includes a variety of specialist simulation software and detailed figures from the book. (Author)

Hematopoietic stem cells : Self-renewing or aging?

NARCIS (Netherlands)

de Haan, G

2002-01-01

Stem cells are defined by their extensive self-renewal properties, and yet there is abundant evidence of erosion of stem cell functioning during aging. Whereas intracellular repair and protection mechanisms determine the lifespan of an individual cell, here an argument is made that somatic stem

Power Electronics for Renewable Energy Systems

DEFF Research Database (Denmark)

Choi, U. M.; Lee, K. B.; Blaabjerg, Frede

2012-01-01

The use of renewable energy sources are increased because of the depletion of natural resources and the increasing pollution level from energy production. The wind energy and the solar energy are most widely used among the renewable energy sources. Power electronics is needed in almost all kinds...... of renewable energy system. It controls the renewable source and interfaces with the load effectively, which can be grid-connected or van work in stand-alone mode. In this presentation, overview of wind and photovoltaic energy systems are introduced. Next, the power electronic circuits behind the most common...

Probabilistic generation assessment system of renewable energy in Korea

Directory of Open Access Journals (Sweden)

Yeonchan Lee

2016-01-01

Full Text Available This paper proposes probabilistic generation assessment system introduction of renewable energy generators. This paper is focused on wind turbine generator and solar cell generator. The proposed method uses an assessment model based on probabilistic model considering uncertainty of resources (wind speed and solar radiation. Equivalent generation function of the wind and solar farms are evaluated. The equivalent generation curves of wind farms and solar farms are assessed using regression analysis method using typical least square method from last actual generation data for wind farms. The proposed model is applied to Korea Renewable Generation System of 8 grouped 41 wind farms and 9 grouped around 600 solar farms in South Korea.

Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.

Science.gov (United States)

Kubota, Hiroshi; Wu, Xin; Goodyear, Shaun M; Avarbock, Mary R; Brinster, Ralph L

2011-08-01

Previous studies suggest that exogenous factors crucial for spermatogonial stem cell (SSC) self-renewal are conserved among several mammalian species. Since glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are critical for rodent SSC self-renewal, we hypothesized that they might promote self-renewal of nonrodent SSCs. Therefore, we cultured testicular germ cells from prepubertal rabbits in the presence of GDNF and FGF2 and found they proliferated indefinitely as cellular clumps that displayed characteristics previously identified for rodent SSCs. The rabbit germ cells could not be maintained on mouse embryonic fibroblast (STO) feeders that support rodent SSC self-renewal in vitro but were rather supported on mouse yolk sac-derived endothelial cell (C166) feeder layers. Proliferation of rabbit germ cells was dependent on GDNF. Of critical importance was that clump-forming rabbit germ cells colonized seminiferous tubules of immunodeficient mice, proliferated for at least 6 mo, while retaining an SSC phenotype in the testes of recipient mice, indicating that they were rabbit SSCs. This study demonstrates that GDNF is a mitogenic factor promoting self-renewal that is conserved between rodent and rabbit SSCs; with an evolutionary separation of ∼ 60 million years. These findings provide a foundation to study the mechanisms governing SSC self-renewal in nonrodent species.

Energy Systems With Renewable Hydrogen Compared to Direct Use of Renewable Energy in Austria

International Nuclear Information System (INIS)

Gerfried Jungmeier; Kurt Konighofer; Josef Spitzer; R Haas; A Ajanovic

2006-01-01

The current Austrian energy system has a renewable energy share of 20% - 11% hydropower and 9 % biomass - of total primary energy consumption. Whereas a possible future introduction of renewable hydrogen must be seen in the context of current energy policies in Austria e.g. increase of energy efficiency and use of renewable energy, reduction of greenhouse gas emissions. The aim of the research project is a life cycle based comparison of energy systems with renewable hydrogen from hydropower, wind, photovoltaic and biomass compared to the direct use of renewable energy for combined heat and power applications and transportation services. In particular this paper focuses on the main question, if renewable energy should be used directly or indirectly via renewable hydrogen. The assessment is based on a life cycle approach to analyse the energy efficiency, the material demand, the greenhouse gas emissions and economic aspects e.g. energy costs and some qualitative aspects e.g. energy service. The overall comparison of the considered energy systems for transportation service and combined heat and electricity application shows, that renewable hydrogen might be beneficial mainly for transportation services, if the electric vehicle will not be further developed to a feasibly wide-spread application for transportation service in future. For combined heat and electricity production there is no advantage of renewable hydrogen versus the direct use of renewable energy. Conclusions for Austria are therefore: 1) renewable hydrogen is an interesting energy carrier and might play an important role in a future sustainable Austrian energy system; 2) renewable hydrogen applications look most promising in the transportation sector; 3) renewable hydrogen applications will be of low importance for combined heat and electricity applications, as existing technologies for direct use of renewable energy for heat and electricity are well developed and very efficient; 4) In a future '100

Resveratrol Enhances Self-Renewal of Mouse Embryonic Stem Cells.

Science.gov (United States)

Li, Na; Du, Zhaoyu; Shen, Qiaoyan; Lei, Qijing; Zhang, Ying; Zhang, Mengfei; Hua, Jinlian

2017-07-01

Resveratrol (RSV) has been shown to affect the differentiation of several types of stem cells, while the detailed mechanism is elusive. Here, we aim to investigate the function of RSV in self-renewal of mouse embryonic stem cells (ESCs) and the related mechanisms. In contrast with its reported roles, we found unexpectedly that differentiated ESCs or iPSCs treated by RSV would not show further differentiation, but regained a naïve pluripotency state with higher expressions of core transcriptional factors and with the ability to differentiate into all three germ layers when transplanted in vivo. In accordance with these findings, RSV also enhanced cell cycle progression of ESCs via regulating cell cycle-related proteins. Finally, enhanced activation of JAK/STAT3 signaling pathway and suppressed activation of mTOR were found essential in enhancing the self-renewal of ESCs by RSV. Our finding discovered a novel function of RSV in enhancing the self-renewal of ESCs, and suggested that the timing of treatment and concentration of RSV determined the final effect of it. Our work may contribute to understanding of RSV in the self-renewal maintenance of pluripotent stem cells, and may also provide help to the generation and maintenance of iPSCs in vitro. J. Cell. Biochem. 118: 1928-1935, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

The geopolitics of renewables; exploring the political implications of renewable energy systems

NARCIS (Netherlands)

Scholten, D.J.; Bosman, Rick

2016-01-01

This paper explores the potential political implications of the geographic and technical characteristics of renewable energy systems. This is done through a thought experiment that imagines a purely renewable based energy system, keeping all else equal. We start by noting that all countries have

Renewable sustainable biocatalyzed electricity production in a photosynthetic algal microbial fuel cell (PAMFC).

Science.gov (United States)

Strik, David P B T B; Terlouw, Hilde; Hamelers, Hubertus V M; Buisman, Cees J N

2008-12-01

Electricity production via solar energy capturing by living higher plants and microalgae in combination with microbial fuel cells are attractive because these systems promise to generate useful energy in a renewable, sustainable, and efficient manner. This study describes the proof of principle of a photosynthetic algal microbial fuel cell (PAMFC) based on naturally selected algae and electrochemically active microorganisms in an open system and without addition of instable or toxic mediators. The developed solar-powered PAMFC produced continuously over 100 days renewable biocatalyzed electricity. The sustainable performance of the PAMFC resulted in a maximum current density of 539 mA/m2 projected anode surface area and a maximum power production of 110 mW/m2 surface area photobioreactor. The energy recovery of the PAMFC can be increased by optimization of the photobioreactor, by reducing the competition from non-electrochemically active microorganisms, by increasing the electrode surface and establishment of a further-enriched biofilm. Since the objective is to produce net renewable energy with algae, future research should also focus on the development of low energy input PAMFCs. This is because current algae production systems have energy inputs similar to the energy present in the outcoming valuable products.

Renewable Energy Tracking Systems

Science.gov (United States)

Renewable energy generation ownership can be accounted through tracking systems. Tracking systems are highly automated, contain specific information about each MWh, and are accessible over the internet to market participants.

wnt3a but not wnt11 supports self-renewal of embryonic stem cells

International Nuclear Information System (INIS)

Singla, Dinender K.; Schneider, David J.; LeWinter, Martin M.; Sobel, Burton E.

2006-01-01

wnt proteins (wnts) promote both differentiation of midbrain dopaminergic cells and self-renewal of haematopoietic stem cells. Mouse embryonic stem (ES) cells can be maintained and self-renew on mouse feeder cell layers or in media containing leukemia inhibitory factor (LIF). However, the effects of wnts on ES cells self-renewal and differentiation are not clearly understood. In the present study, we found that conditioned medium prepared from L cells expressing wnt3a can replace feeder cell layers and medium containing LIF in maintaining ES cells in the proliferation without differentiation (self-renewal) state. By contrast, conditioned medium from NIH3T3 cells expressing wnt11 did not. Alkaline phosphatase staining and compact colony formation were used as criteria of cells being in the undifferentiated state. ES cells maintained in medium conditioned by Wnt3a expressing cells underwent freezing and thawing while maintaining properties seen with LIF maintained ES cells. Purified wnt3a did not maintain self-renewal of ES cells for prolonged intervals. Thus, other factors in the medium conditioned by wnt3a expressing cells may have contributed to maintenance of ES cells in a self-renewal state. Pluripotency of ES cells was determined with the use of embryoid bodies in vitro. PD98059, a MEK specific inhibitor, promoted the growth of undifferentiated ES cells maintained in conditioned medium from wnt3a expressing cells. By contrast, the P38 MAPK inhibitor SB230580 did not, suggesting a role for the MEK pathway in self-renewal and differentiation of ES cells maintained in the wnt3a cell conditioned medium. Thus, our results show that conditioned medium from wnt3a but not wnt11 expressing cells can maintain ES cells in self-renewal and in a pluripotent state

Comparison between hybrid renewable energy systems in Saudi Arabia

Directory of Open Access Journals (Sweden)

Hisham El Khashab

2015-05-01

This paper investigates RE sources applications at Yanbu, Saudi Arabia, besides a simulation using HOMER software to three proposed systems newly erected in Yanbu Industrial College Renewable Energy (RE lab. The lab represents a hybrid system, composed of PV, wind turbine, and Fuel cell systems. The cost of energy is compared in the three systems to have an actual estimation for RE in developing countries. The climatic variations at Yanbu that is located on the west coast of Saudi Arabia are considered.

The self-renewal signaling pathways utilized by gastric cancer stem cells.

Science.gov (United States)

Fu, Ying; Li, Hui; Hao, Xishan

2017-04-01

Gastric cancer is a leading cause of cancer-related mortality worldwide. Cancer stem cells are the source of tumor recurrence and metastasis. Self-renewal is a marker of cancer stem cells and also the basis of long-lasting survival and tumor progression. Although the mechanism of gastric cancer stem cell self-renewal is not clear, there are several signaling pathways and environmental factors known to be involved. This mini review describes recent developments in the self-renewal signaling pathway of gastric cancer stem cell research. Advancements made in this field of research will likely support the development of novel therapeutic strategies for gastric cancer.

Special issue on advancing grid-connected renewable generation systems

DEFF Research Database (Denmark)

Blaabjerg, Frede; Yang, Yongheng

2017-01-01

Renewables are heavily involved in power generation, as an essential component for today’s energy paradigm. Energy structure—both national and international—has been undergoing significant changes over the past few decades. For instance, in Denmark, power generation is shifting from fossil......-fuel-based to renewable-based in terms of energy sources, from centralized to decentralized in terms of architectures, and from sole to miscellaneous in terms of energy varieties [1]. In this energy evolution, the power electronic technology plays an enabling role in the integration and advancements of renewables......—such as wind turbine, photovoltaics, fuel cells, and other emerging energy systems. At the same time, various control strategies are necessary to guide the energy integration (i.e., to enhance the energy transition), and on the other hand, to flexibly, reliably, and efficiently utilize the energy. Tremendous...

STAT5-mediated self-renewal of normal hematopoietic and leukemic stem cells

NARCIS (Netherlands)

Schepers, Hein; Wierenga, Albertus T. J.; Vellenga, Edo; Schuringa, Jan Jacob

2012-01-01

The level of transcription factor activity critically regulates cell fate decisions such as hematopoietic stem cell self-renewal and differentiation. The balance between hematopoietic stem cell self-renewal and differentiation needs to be tightly controlled, as a shift toward differentiation might

Self-renewing Monolayer of Primary Colonic or Rectal Epithelial CellsSummary

Directory of Open Access Journals (Sweden)

Yuli Wang

2017-07-01

Full Text Available Background & Aims: Three-dimensional organoid culture has fundamentally changed the in vitro study of intestinal biology enabling novel assays; however, its use is limited because of an inaccessible luminal compartment and challenges to data gathering in a three-dimensional hydrogel matrix. Long-lived, self-renewing 2-dimensional (2-D tissue cultured from primary colon cells has not been accomplished. Methods: The surface matrix and chemical factors that sustain 2-D mouse colonic and human rectal epithelial cell monolayers with cell repertoires comparable to that in vivo were identified. Results: The monolayers formed organoids or colonoids when placed in standard Matrigel culture. As with the colonoids, the monolayers exhibited compartmentalization of proliferative and differentiated cells, with proliferative cells located near the peripheral edges of growing monolayers and differentiated cells predominated in the central regions. Screening of 77 dietary compounds and metabolites revealed altered proliferation or differentiation of the murine colonic epithelium. When exposed to a subset of the compound library, murine organoids exhibited similar responses to that of the monolayer but with differences that were likely attributable to the inaccessible organoid lumen. The response of the human primary epithelium to a compound subset was distinct from that of both the murine primary epithelium and human tumor cells. Conclusions: This study demonstrates that a self-renewing 2-D murine and human monolayer derived from primary cells can serve as a physiologically relevant assay system for study of stem cell renewal and differentiation and for compound screening. The platform holds transformative potential for personalized and precision medicine and can be applied to emerging areas of disease modeling and microbiome studies. Keywords: Colonic Epithelial Cells, Monolayer, Organoids, Compound Screening

Battery storage for supplementing renewable energy systems

Energy Technology Data Exchange (ETDEWEB)

None, None

2009-01-18

The battery storage for renewable energy systems section of the Renewable Energy Technology Characterizations describes structures and models to support the technical and economic status of emerging renewable energy options for electricity supply.

Albumin-associated lipids regulate human embryonic stem cell self-renewal.

Directory of Open Access Journals (Sweden)

Francesc R Garcia-Gonzalo

Full Text Available BACKGROUND: Although human embryonic stem cells (hESCs hold great promise as a source of differentiated cells to treat several human diseases, many obstacles still need to be surmounted before this can become a reality. First among these, a robust chemically-defined system to expand hESCs in culture is still unavailable despite recent advances in the understanding of factors controlling hESC self-renewal. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we attempted to find new molecules that stimulate long term hESC self-renewal. In order to do this, we started from the observation that a commercially available serum replacement product has a strong positive effect on the expansion of undifferentiated hESCs when added to a previously reported chemically-defined medium. Subsequent experiments demonstrated that the active ingredient within the serum replacement is lipid-rich albumin. Furthermore, we show that this activity is trypsin-resistant, strongly suggesting that lipids and not albumin are responsible for the effect. Consistent with this, lipid-poor albumin shows no detectable activity. Finally, we identified the major lipids bound to the lipid-rich albumin and tested several lipid candidates for the effect. CONCLUSIONS/SIGNIFICANCE: Our discovery of the role played by albumin-associated lipids in stimulating hESC self-renewal constitutes a significant advance in the knowledge of how hESC pluripotency is maintained by extracellular factors and has important applications in the development of increasingly chemically defined hESC culture systems.

Integration of Renewable Generation in Power System Defence Plans

DEFF Research Database (Denmark)

Das, Kaushik

Increasing levels of penetration of wind power and other renewable generations in European power systems pose challenges to power system security. The power system operators are continuously challenged especially when generations from renewables are high thereby reducing online capacity of conven......Increasing levels of penetration of wind power and other renewable generations in European power systems pose challenges to power system security. The power system operators are continuously challenged especially when generations from renewables are high thereby reducing online capacity......, one of them being the North East area with high share of wind power generation.The aim of this study is to investigate how renewable generations like wind power can contribute to the power system defence plans. This PhD project “Integration of Renewable Generation in Power System Defence Plans...

The C. elegans engrailed homolog ceh-16 regulates the self-renewal expansion division of stem cell-like seam cells.

Science.gov (United States)

Huang, Xinxin; Tian, E; Xu, Yanhua; Zhang, Hong

2009-09-15

Stem cells undergo symmetric and asymmetric division to maintain the dynamic equilibrium of the stem cell pool and also to generate a variety of differentiated cells. The homeostatic mechanism controlling the choice between self-renewal and differentiation of stem cells is poorly understood. We show here that ceh-16, encoding the C. elegans ortholog of the transcription factor Engrailed, controls symmetric and asymmetric division of stem cell-like seam cells. Loss of function of ceh-16 causes certain seam cells, which normally undergo symmetric self-renewal expansion division with both daughters adopting the seam cell fate, to divide asymmetrically with only one daughter retaining the seam cell fate. The human engrailed homolog En2 functionally substitutes the role of ceh-16 in promoting self-renewal expansion division of seam cells. Loss of function of apr-1, encoding the C. elegans homolog of the Wnt signaling component APC, results in transformation of self-renewal maintenance seam cell division to self-renewal expansion division, leading to seam cell hyperplasia. The apr-1 mutation suppresses the seam cell division defect in ceh-16 mutants. Our study reveals that ceh-16 interacts with the Wnt signaling pathway to control the choice between self-renewal expansion and maintenance division and also demonstrates an evolutionarily conserved function of engrailed in promoting cell proliferation.

Soft computing in green and renewable energy systems

Energy Technology Data Exchange (ETDEWEB)

Gopalakrishnan, Kasthurirangan [Iowa State Univ., Ames, IA (United States). Iowa Bioeconomy Inst.; US Department of Energy, Ames, IA (United States). Ames Lab; Kalogirou, Soteris [Cyprus Univ. of Technology, Limassol (Cyprus). Dept. of Mechanical Engineering and Materials Sciences and Engineering; Khaitan, Siddhartha Kumar (eds.) [Iowa State Univ. of Science and Technology, Ames, IA (United States). Dept. of Electrical Engineering and Computer Engineering

2011-07-01

Soft Computing in Green and Renewable Energy Systems provides a practical introduction to the application of soft computing techniques and hybrid intelligent systems for designing, modeling, characterizing, optimizing, forecasting, and performance prediction of green and renewable energy systems. Research is proceeding at jet speed on renewable energy (energy derived from natural resources such as sunlight, wind, tides, rain, geothermal heat, biomass, hydrogen, etc.) as policy makers, researchers, economists, and world agencies have joined forces in finding alternative sustainable energy solutions to current critical environmental, economic, and social issues. The innovative models, environmentally benign processes, data analytics, etc. employed in renewable energy systems are computationally-intensive, non-linear and complex as well as involve a high degree of uncertainty. Soft computing technologies, such as fuzzy sets and systems, neural science and systems, evolutionary algorithms and genetic programming, and machine learning, are ideal in handling the noise, imprecision, and uncertainty in the data, and yet achieve robust, low-cost solutions. As a result, intelligent and soft computing paradigms are finding increasing applications in the study of renewable energy systems. Researchers, practitioners, undergraduate and graduate students engaged in the study of renewable energy systems will find this book very useful. (orig.)

Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells.

Science.gov (United States)

Soucie, Erinn L; Weng, Ziming; Geirsdóttir, Laufey; Molawi, Kaaweh; Maurizio, Julien; Fenouil, Romain; Mossadegh-Keller, Noushine; Gimenez, Gregory; VanHille, Laurent; Beniazza, Meryam; Favret, Jeremy; Berruyer, Carole; Perrin, Pierre; Hacohen, Nir; Andrau, J-C; Ferrier, Pierre; Dubreuil, Patrice; Sidow, Arend; Sieweke, Michael H

2016-02-12

Differentiated macrophages can self-renew in tissues and expand long term in culture, but the gene regulatory mechanisms that accomplish self-renewal in the differentiated state have remained unknown. Here we show that in mice, the transcription factors MafB and c-Maf repress a macrophage-specific enhancer repertoire associated with a gene network that controls self-renewal. Single-cell analysis revealed that, in vivo, proliferating resident macrophages can access this network by transient down-regulation of Maf transcription factors. The network also controls embryonic stem cell self-renewal but is associated with distinct embryonic stem cell-specific enhancers. This indicates that distinct lineage-specific enhancer platforms regulate a shared network of genes that control self-renewal potential in both stem and mature cells. Copyright © 2016, American Association for the Advancement of Science.

Stem cell signaling. An integral program for tissue renewal and regeneration : Wnt signaling and stem cell control

NARCIS (Netherlands)

Clevers, Hans; Loh, Kyle M; Nusse, Roel

2014-01-01

Stem cells fuel tissue development, renewal, and regeneration, and these activities are controlled by the local stem cell microenvironment, the "niche." Wnt signals emanating from the niche can act as self-renewal factors for stem cells in multiple mammalian tissues. Wnt proteins are lipid-modified,

Endogenous production of fibronectin is required for self-renewal of cultured mouse embryonic stem cells.

Science.gov (United States)

Hunt, Geoffrey C; Singh, Purva; Schwarzbauer, Jean E

2012-09-10

Pluripotent cells are attached to the extracellular matrix (ECM) as they make cell fate decisions within the stem cell niche. Here we show that the ubiquitous ECM protein fibronectin is required for self-renewal decisions by cultured mouse embryonic stem (mES) cells. Undifferentiated mES cells produce fibronectin and assemble a fibrillar matrix. Increasing the level of substrate fibronectin increased cell spreading and integrin receptor signaling through focal adhesion kinase, while concomitantly inducing the loss of Nanog and Oct4 self-renewal markers. Conversely, reducing fibronectin production by mES cells growing on a feeder-free gelatin substrate caused loss of cell adhesion, decreased integrin signaling, and decreased expression of self-renewal markers. These effects were reversed by providing the cells with exogenous fibronectin, thereby restoring adhesion to the gelatin substrate. Interestingly, mES cells do not adhere directly to the gelatin substrate, but rather adhere indirectly through gelatin-bound fibronectin, which facilitates self-renewal via its effects on cell adhesion. These results provide new insights into the mechanism of regulation of self-renewal by growth on a gelatin-coated surface. The effects of increasing or decreasing fibronectin levels show that self-renewal depends on an intermediate level of cell-fibronectin interactions. By providing cell adhesive signals that can act with other self-renewal factors to maintain mES cell pluripotency, fibronectin is therefore a necessary component of the self-renewal signaling pathway in culture. Copyright © 2012 Elsevier Inc. All rights reserved.

Renewable sustainable biocatalyzed electricity production in a photosynthetic algal microbial fuel cell (PAMFC)

Energy Technology Data Exchange (ETDEWEB)

Strik, David P.B.T.B.; Terlouw, Hilde; Hamelers, Hubertus V.M.; Buisman, Cees J.N. [Wageningen Univ. (Netherlands). Sub-Dept. of Environmental Technology

2008-12-15

Electricity production via solar energy capturing by living higher plants and microalgae in combination with microbial fuel cells are attractive because these systems promise to generate useful energy in a renewable, sustainable, and efficient manner. This study describes the proof of principle of a photosynthetic algal microbial fuel cell (PAMFC) based on naturally selected algae and electrochemically active microorganisms in an open system and without addition of instable or toxic mediators. The developed solar-powered PAMFC produced continuously over 100 days renewable biocatalyzed electricity. The sustainable performance of the PAMFC resulted in a maximum current density of 539 mA/m{sup 2} projected anode surface area and a maximum power production of 110 mW/m{sup 2} surface area photobioreactor. The energy recovery of the PAMFC can be increased by optimization of the photobioreactor, by reducing the competition from non-electrochemically active microorganisms, by increasing the electrode surface and establishment of a further-enriched biofilm. Since the objective is to produce net renewable energy with algae, future research should also focus on the development of low energy input PAMFCs. This is because current algae production systems have energy inputs similar to the energy present in the outcoming valuable products. (orig.)

Input-output analysis for installing renewable energy systems

International Nuclear Information System (INIS)

Itoh, Y.; Nakata, T.

2004-01-01

Renewable energy facilities have been installed in many regions, because of their possibility to be an alternative to fossil fuels for mitigating global warming. Besides the profitability of renewable energy businesses, indirect economic effects of installing renewable energy facilities should be clarified. This study examines the possibility that the renewable energy facilities give renewed impetus to regional economic progress. The economic effects are analysed with input-output techniques in a rural area in Japan. As a consequence, both positive and negative effects on the rural economy are derived. In addition, we will focus on the changes in sectors such as construction, business services, banking, etc. as a result of economic activities for renewable systems. The business benefits of renewable energy system are discussed. (author)

Smart power systems and renewable energy system integration

CERN Document Server

2016-01-01

This monograph presents a wider spectrum of researches, developments, and case specific studies in the area of smart power systems and integration of renewable energy systems. The book will be for the benefit of a wider audience including researchers, postgraduate students, practicing engineers, academics, and regulatory policy makers. It covers a wide range of topics from fundamentals, and modelling and simulation aspects of traditional and smart power systems to grid integration of renewables; Micro Grids; challenges in planning and operation of a smart power system; risks, security, and stability in smart operation of a power system; and applied research in energy storage. .

Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal.

Science.gov (United States)

Kanatsu-Shinohara, Mito; Tanaka, Takashi; Ogonuki, Narumi; Ogura, Atsuo; Morimoto, Hiroko; Cheng, Pei Feng; Eisenman, Robert N; Trumpp, Andreas; Shinohara, Takashi

2016-12-01

Myc plays critical roles in the self-renewal division of various stem cell types. In spermatogonial stem cells (SSCs), Myc controls SSC fate decisions because Myc overexpression induces enhanced self-renewal division, while depletion of Max, a Myc-binding partner, leads to meiotic induction. However, the mechanism by which Myc acts on SSC fate is unclear. Here we demonstrate a critical link between Myc/Mycn gene activity and glycolysis in SSC self-renewal. In SSCs, Myc/Mycn are regulated by Foxo1, whose deficiency impairs SSC self-renewal. Myc/Mycn-deficient SSCs not only undergo limited self-renewal division but also display diminished glycolytic activity. While inhibition of glycolysis decreased SSC activity, chemical stimulation of glycolysis or transfection of active Akt1 or Pdpk1 (phosphoinositide-dependent protein kinase 1 ) augmented self-renewal division, and long-term SSC cultures were derived from a nonpermissive strain that showed limited self-renewal division. These results suggested that Myc-mediated glycolysis is an important factor that increases the frequency of SSC self-renewal division. © 2016 Kanatsu-Shinohara et al.; Published by Cold Spring Harbor Laboratory Press.

The Palm Desert renewable [hydrogen] transportation system

Energy Technology Data Exchange (ETDEWEB)

Chamberlin, C.E.; Lehman, P. [Humboldt State Univ., Arcata, CA (United States). Schatz Energy Research Center

1998-08-01

This paper describes the Schatz Energy Research Center (SERC) progress on the Palm Desert Renewable Hydrogen Transportation System Project for the period June 1997 through May 1998. The project began in March 1996. The goal of the Palm Desert Project is to develop a clean and sustainable transportation system for a community. The project demonstrates the practical utility of hydrogen as a transportation fuel and the proton exchange membrane (PEM) fuel cell as a vehicle power system. The project includes designing and building 4 fuel cell powered vehicles, a solar hydrogen generating and refueling station, and a fuel cell vehicle diagnostic center. Over this last year, SERC has built a fuel cell powered neighborhood electric vehicle and delivered it to the City of Palm Desert. The design of the hydrogen refueling station is near completion and it is anticipated that construction will be complete in the fall of 1998. The vehicles are currently being refueled at a temporary refueling station. The diagnostic center is being designed and maintenance procedures as well as computer diagnostic programs for the fuel cell vehicles are being developed. City employees are driving the vehicles daily and monitoring data are being collected. The drivers are pleased with the performance of the vehicles.

Integrated Nuclear-Renewable Energy Systems: Foundational Workshop Report

International Nuclear Information System (INIS)

2014-01-01

The U.S. Department of Energy (DOE) recognizes the need to transform the energy infrastructure of the U.S. and elsewhere to systems that can drastically reduce environmental impacts in an efficient and economically viable manner while utilizing both hydrocarbon resources and clean energy generation sources. Thus, DOE is supporting research and development that could lead to more efficient utilization of clean energy generation sources, including renewable and nuclear options. A concept being advanced by the DOE Offices of Nuclear Energy (NE) and Energy Efficiency and Renewable Energy (EERE) is tighter coupling of nuclear and renewable energy sources in a manner that produces new energy currency for the combined electricity grid, industrial manufacturing, and the transportation energy sectors. This integration concept has been referred to as a ''hybrid system'' that is capable of providing the right type of energy, at the right time, in the right place. At the direction of DOE-NE and DOE-EERE leadership, project leads at Idaho National Laboratory (INL), National Renewable Energy Laboratory (NREL) and Massachusetts Institute of Technology (MIT) have identified and engaged stakeholders in discussing integrated energy systems that would optimize renewable and nuclear energy integration on a region-by-region basis. Subsequent work will entail conduct of technical, economic, environmental and socio-political evaluations of the leading integrated system options based on a set of criteria established with stakeholder input. The Foundational Workshop for Integrated Nuclear - Renewable Energy Systems was organized around the following objectives: 1. Identify and refine priority region-specific opportunities for integrated nuclear-renewable energy systems in the U.S.; 2. Select Figures of Merit (FOM) to rank and prioritize candidate systems; 3. Discuss enabling technology development needs; 4. Identify analysis requirements, capabilities and gaps to

Renewable energy systems the choice and modeling of 100% renewable solutions

CERN Document Server

Lund, Henrik

2009-01-01

How can society quickly convert to renewable energy? Can worldwide energy needs ever be met through 100% renewable sources? The answers to these questions rest largely on the perception of choice in the energy arena. It is of pivotal importance that engineers, researchers and policymakers understand what choices are available, and reasonable, when considering the design and deployment of new energy systems. The mission of this new book, written by one of the world's foremost experts in renewable power, is to arm these professionals with the tools and methodologies necessary to make smart choic

Hydrogen, fuel cells and renewable energy integration in islands

International Nuclear Information System (INIS)

Bauen, A.; Hart, D.; Foradini, F.; Hart, D.

2002-01-01

Remote areas such as islands rely on costly and highly polluting diesel and heavy fuel oil for their electricity supply. This paper explored the opportunities for exploiting economically and environmentally viable renewable energy sources, in particular hydrogen storage, on such islands. In particular, this study focused on addressing the challenge of matching energy supply with demand and with technical issues regarding weak grids that are hindered with high steady state voltage levels and voltage fluctuations. The main technical characteristics of integrated renewable energy and hydrogen systems were determined by modelling a case study for the island of El Hierro (Canary Islands). The paper referred to the challenges regarding the technical and economic viability of such systems and their contribution to the economic development of remote communities. It was noted that energy storage plays an important role in addressing supply and demand issues by offering a way to control voltage and using surplus electricity at times of low load. Electrical energy can be stored in the form of potential or chemical energy. New decentralized generation technologies have also played a role in improving the energy efficiency of renewable energy sources. The feasibility of using hydrogen for energy storage was examined with particular reference to fuel-cell based energy supply in isolated island communities. 4 refs., 5 figs

REST controls self-renewal and tumorigenic competence of human glioblastoma cells.

Directory of Open Access Journals (Sweden)

Luciano Conti

Full Text Available The Repressor Element 1 Silencing Transcription factor (REST/NRSF is a master repressor of neuronal programs in non-neuronal lineages shown to function as a central regulator of developmental programs and stem cell physiology. Aberrant REST function has been associated with a number of pathological conditions. In cancer biology, REST has been shown to play a tumor suppressor activity in epithelial cancers but an oncogenic role in brain childhood malignancies such as neuroblastoma and medulloblastoma. Here we examined REST expression in human glioblastoma multiforme (GBM specimens and its role in GBM cells carrying self-renewal and tumorigenic competence. We found REST to be expressed in GBM specimens, its presence being particularly enriched in tumor cells in the perivascular compartment. Significantly, REST is highly expressed in self-renewing tumorigenic-competent GBM cells and its knock down strongly reduces their self-renewal in vitro and tumor-initiating capacity in vivo and affects levels of miR-124 and its downstream targets. These results indicate that REST contributes to GBM maintenance by affecting its self-renewing and tumorigenic cellular component and that, hence, a better understanding of these circuitries in these cells might lead to new exploitable therapeutic targets.

Introduction to Renewable Energy Systems

DEFF Research Database (Denmark)

Ma, Ke; Yang, Yongheng; Blaabjerg, Frede

2014-01-01

. It is concluded that as the quick development of renewable energy, wind power and PV power both show great potential to be largely integrated into the power grid. Power electronics is playing essential role in both of the systems to achieve more controllable, efficient, and reliable energy production......In this chapter, the state-of-the-arts developments of renewable energy are reviewed in respect to the installed power and market share, where wind power and photovoltaic power generation are the main focuses due to the fast growing speed and large share of installed capacity. Some basic principles...... of operation, mission profiles, as well as power electronics solutions and corresponding controls are discussed respectively in the case of wind power and photovoltaic power systems. Finally a few development trends for renewable energy conversions are also given from a power electronics point of view...

[Tricostantin A inhibits self-renewal of breast cancer stem cells in vitro].

Science.gov (United States)

Peng, Li; Li, Fu-Xi; Shao, Wen-Feng; Xiong, Jing-Bo

2013-10-01

To investigate the effect of tricostantin A (TSA) on self-renewal of breast cancer stem cells and explore the mechanisms. Breast cancer cell lines MDA-MB-468, MDA-MB-231, MCF-7 and SKBR3 were cultured in suspension and treated with different concentrations of TSA for 7 days, using 0.1% DMSO as the control. Secondary mammosphere formation efficiency and percentage of CD44(+)/CD24(-) sub-population in the primary mammospheres were used to evaluate the effects of TSA on self-renewal of breast cancer stem cells. The breast cancer stem cell surface marker CD44(+)/CD24(-) and the percentage of apoptosis in the primary mammospheres were assayed using flow cytometry. The mRNA expressions of Nanog, Sox2 and Oct4 in the primary mammospheres were assayed with quantitative PCR. TSA at both 100 and 500 nmol/L, but not at 10 nmol/L, partially inhibited the self-renewal of breast cancer stem cells from the 4 cell lines. TSA at 500 nmol/L induced cell apoptosis in the primary mammospheres. TSA down-regulated the mRNA expression of Nanog and Sox2 in the primary mammospheres. TSA can partially inhibit the self-renewal of breast cancer stem cells through a mechanism involving the down-regulation of Nanog and Sox2 expression, indicating the value of combined treatments with low-dose TSA and other anticancer drugs to achieve maximum inhibition of breast cancer stem cell self-renewal. The core transcriptional factor of embryonic stem cells Nanog and Sox2 can be potential targets of anticancer therapy.

Life-cycle analysis of renewable energy systems

DEFF Research Database (Denmark)

Sørensen, Bent

1994-01-01

An imlementation of life-cycle analysis (LCA) for energy systems is presented and applied to two renewable energy systems (wind turbines and building-integrated photovoltaic modules) and compared with coal plants......An imlementation of life-cycle analysis (LCA) for energy systems is presented and applied to two renewable energy systems (wind turbines and building-integrated photovoltaic modules) and compared with coal plants...

Renewable energy integration into the Spanish power system

International Nuclear Information System (INIS)

Duvison Garcia, Miguel R.; Rivas Cuenca, Ana

2013-01-01

The increase in renewable energy sources in the Spanish peninsular system, along with the installation of other technologies represents a challenge due to the particularities of this type of technologies. Innovative solutions and new operation paradigms may be needed in order to cope with these challenges. Grid codes must incorporate new specifications for these technologies and demand management strategies must be incorporated in control centers in order to balance the system, maximize renewable production and maintain system security. In real time, the most significant improvements that ease integration of renewable resources are the introduction of observability and controllability, which is especially important in dealing with the problem of system balancing and the impact of renewable energy on matching generation and demand. In this regard the commissioning of a control center specifically for management of these technologies have been taken in the Spanish electrical system in order to integrate the maximum amount of renewable energy

Integrated Nuclear-Renewable Energy Systems: Foundational Workshop Report

Energy Technology Data Exchange (ETDEWEB)

Bragg-Sitton, Shannon [Idaho National Lab. (INL), Idaho Falls, ID (United States); Boardman, Richard [Idaho National Lab. (INL), Idaho Falls, ID (United States); Ruth, Mark [National Renewable Energy Lab. (NREL), Golden, CO (United States); Zinaman, Owen [National Renewable Energy Lab. (NREL), Golden, CO (United States); Forsberg, Charles [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Collins, John [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2014-08-01

The U.S. Department of Energy (DOE) recognizes the need to transform the energy infrastructure of the U.S. and elsewhere to systems that can drastically reduce environmental impacts in an efficient and economically viable manner while utilizing both hydrocarbon resources and clean energy generation sources. Thus, DOE is supporting research and development that could lead to more efficient utilization of clean energy generation sources, including renewable and nuclear options. A concept being advanced by the DOE Offices of Nuclear Energy (NE) and Energy Efficiency and Renewable Energy (EERE) is tighter coupling of nuclear and renewable energy sources in a manner that produces new energy currency for the combined electricity grid, industrial manufacturing, and the transportation energy sectors. This integration concept has been referred to as a “hybrid system” that is capable of providing the right type of energy, at the right time, in the right place. At the direction of DOE-NE and DOE-EERE leadership, project leads at Idaho National Laboratory (INL), National Renewable Energy Laboratory (NREL) and Massachusetts Institute of Technology (MIT) have identified and engaged stakeholders in discussing integrated energy systems that would optimize renewable and nuclear energy integration on a region-by-region basis. Subsequent work will entail conduct of technical, economic, environmental and socio-political evaluations of the leading integrated system options based on a set of criteria established with stakeholder input. The Foundational Workshop for Integrated Nuclear – Renewable Energy Systems was organized around the following objectives: 1. Identify and refine priority region-specific opportunities for integrated nuclear-renewable energy systems in the U.S.; 2. Select Figures of Merit (FOM) to rank and prioritize candidate systems; 3. Discuss enabling technology development needs; 4. Identify analysis requirements, capabilities and gaps to estimate FOM for

Ferritin nanoparticles for improved self-renewal and differentiation of human neural stem cells.

Science.gov (United States)

Lee, Jung Seung; Yang, Kisuk; Cho, Ann-Na; Cho, Seung-Woo

2018-01-01

Biomaterials that promote the self-renewal ability and differentiation capacity of neural stem cells (NSCs) are desirable for improving stem cell therapy to treat neurodegenerative diseases. Incorporation of micro- and nanoparticles into stem cell culture has gained great attention for the control of stem cell behaviors, including proliferation and differentiation. In this study, ferritin, an iron-containing natural protein nanoparticle, was applied as a biomaterial to improve the self-renewal and differentiation of NSCs and neural progenitor cells (NPCs). Ferritin nanoparticles were added to NSC or NPC culture during cell growth, allowing for incorporation of ferritin nanoparticles during neurosphere formation. Compared to neurospheres without ferritin treatment, neurospheres with ferritin nanoparticles showed significantly promoted self-renewal and cell-cell interactions. When spontaneous differentiation of neurospheres was induced during culture without mitogenic factors, neuronal differentiation was enhanced in the ferritin-treated neurospheres. In conclusion, we found that natural nanoparticles can be used to improve the self-renewal ability and differentiation potential of NSCs and NPCs, which can be applied in neural tissue engineering and cell therapy for neurodegenerative diseases.

Lhx2 expression promotes self-renewal of a distinct multipotential hematopoietic progenitor cell in embryonic stem cell-derived embryoid bodies.

Directory of Open Access Journals (Sweden)

Lina Dahl

Full Text Available The molecular mechanisms regulating the expansion of the hematopoietic system including hematopoietic stem cells (HSCs in the fetal liver during embryonic development are largely unknown. The LIM-homeobox gene Lhx2 is a candidate regulator of fetal hematopoiesis since it is expressed in the fetal liver and Lhx2(-/- mice die in utero due to severe anemia. Moreover, expression of Lhx2 in embryonic stem (ES cell-derived embryoid bodies (EBs can lead to the generation of HSC-like cell lines. To further define the role of this transcription factor in hematopoietic regulation, we generated ES cell lines that enabled tet-inducible expression of Lhx2. Using this approach we observed that Lhx2 expression synergises with specific signalling pathways, resulting in increased frequency of colony forming cells in developing EB cells. The increase in growth factor-responsive progenitor cells directly correlates to the efficiency in generating HSC-like cell lines, suggesting that Lhx2 expression induce self-renewal of a distinct multipotential hematopoietic progenitor cell in EBs. Signalling via the c-kit tyrosine kinase receptor and the gp130 signal transducer by IL-6 is necessary and sufficient for the Lhx2 induced self-renewal. While inducing self-renewal of multipotential progenitor cells, expression of Lhx2 inhibited proliferation of primitive erythroid precursor cells and interfered with early ES cell commitment, indicating striking lineage specificity of this effect.

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.

Directory of Open Access Journals (Sweden)

Stephen N Sansom

2009-06-01

Full Text Available Neural stem cell self-renewal, neurogenesis, and cell fate determination are processes that control the generation of specific classes of neurons at the correct place and time. The transcription factor Pax6 is essential for neural stem cell proliferation, multipotency, and neurogenesis in many regions of the central nervous system, including the cerebral cortex. We used Pax6 as an entry point to define the cellular networks controlling neural stem cell self-renewal and neurogenesis in stem cells of the developing mouse cerebral cortex. We identified the genomic binding locations of Pax6 in neocortical stem cells during normal development and ascertained the functional significance of genes that we found to be regulated by Pax6, finding that Pax6 positively and directly regulates cohorts of genes that promote neural stem cell self-renewal, basal progenitor cell genesis, and neurogenesis. Notably, we defined a core network regulating neocortical stem cell decision-making in which Pax6 interacts with three other regulators of neurogenesis, Neurog2, Ascl1, and Hes1. Analyses of the biological function of Pax6 in neural stem cells through phenotypic analyses of Pax6 gain- and loss-of-function mutant cortices demonstrated that the Pax6-regulated networks operating in neural stem cells are highly dosage sensitive. Increasing Pax6 levels drives the system towards neurogenesis and basal progenitor cell genesis by increasing expression of a cohort of basal progenitor cell determinants, including the key transcription factor Eomes/Tbr2, and thus towards neurogenesis at the expense of self-renewal. Removing Pax6 reduces cortical stem cell self-renewal by decreasing expression of key cell cycle regulators, resulting in excess early neurogenesis. We find that the relative levels of Pax6, Hes1, and Neurog2 are key determinants of a dynamic network that controls whether neural stem cells self-renew, generate cortical neurons, or generate basal progenitor cells

Renewable sustainable biocatalyzed electricity production in a photosynthetic algal microbial fuel cell (PAMFC)

NARCIS (Netherlands)

Strik, D.P.B.T.B.; Terlouw, H.; Hamelers, H.V.M.; Buisman, C.J.N.

2008-01-01

Electricity production via solar energy capturing by living higher plants and microalgae in combination with microbial fuel cells are attractive because these systems promise to generate useful energy in a renewable, sustainable, and efficient manner. This study describes the proof of principle of a

Renewable energy systems advanced conversion technologies and applications

CERN Document Server

Luo, Fang Lin

2012-01-01

Energy conversion techniques are key in power electronics and even more so in renewable energy source systems, which require a large number of converters. Renewable Energy Systems: Advanced Conversion Technologies and Applications describes advanced conversion technologies and provides design examples of converters and inverters for renewable energy systems-including wind turbine and solar panel energy systems. Learn Cutting-Edge Techniques for Converters and Inverters Setting the scene, the book begins with a review of the basics of astronomy and Earth physics. It then systematically introduc

Proceedings of World Renewable Energy Congress '99

International Nuclear Information System (INIS)

Kamaruzzaman Sopian; Mohd Yusof Othman; Baharuddin Yatim

2000-01-01

The congress discussed the following subjects, 1. The role of renewable energy in the next millenium; 2. Challenges in the commercialization of renewable energy; 3. The role and agenda for renewable energy towards sustainable development. Topics covered in the technical session were biomass conversion; solar thermal technologies and systems; solar photovoltaic s; renewable energy economics, financing and policy; renewable energy education; climate and the environment; energy and architecture; energy management; wind and hydro technologies and systems; hydrogen and fuel cell

Two distinct mechanisms silence chinmo in Drosophila neuroblasts and neuroepithelial cells to limit their self-renewal.

Science.gov (United States)

Dillard, Caroline; Narbonne-Reveau, Karine; Foppolo, Sophie; Lanet, Elodie; Maurange, Cédric

2018-01-25

Whether common principles regulate the self-renewing potential of neural stem cells (NSCs) throughout the developing central nervous system is still unclear. In the Drosophila ventral nerve cord and central brain, asymmetrically dividing NSCs, called neuroblasts (NBs), progress through a series of sequentially expressed transcription factors that limits self-renewal by silencing a genetic module involving the transcription factor Chinmo. Here, we find that Chinmo also promotes neuroepithelium growth in the optic lobe during early larval stages by boosting symmetric self-renewing divisions while preventing differentiation. Neuroepithelium differentiation in late larvae requires the transcriptional silencing of chinmo by ecdysone, the main steroid hormone, therefore allowing coordination of neural stem cell self-renewal with organismal growth. In contrast, chinmo silencing in NBs is post-transcriptional and does not require ecdysone. Thus, during Drosophila development, humoral cues or tissue-intrinsic temporal specification programs respectively limit self-renewal in different types of neural progenitors through the transcriptional and post-transcriptional regulation of the same transcription factor. © 2018. Published by The Company of Biologists Ltd.

Fibroblast growth factors as regulators of stem cell self-renewal and aging

NARCIS (Netherlands)

Yeoh, Joyce S. G.; de Haan, Gerald

Organ and tissue dysfunction which is readily observable during aging results from a loss of cellular homeostasis and reduced stem cell self-renewal. Over the past 10 years, studies have been aimed at delineating growth factors that will sustain and promote the self-renewal potential of stem cells

The feasibility of synthetic fuels in renewable energy systems

DEFF Research Database (Denmark)

Ridjan, Iva; Mathiesen, Brian Vad; Connolly, D.

2013-01-01

, and other impacts on the environment and biosphere. Hence, it is essential to make a detailed analysis of this sector in order to match the demand and to meet the criteria of a 100% renewable energy system in 2050. The purpose of this article is to identify potential pathways for producing synthetic fuels......, with a specific focus on solid oxide electrolyser cells (SOEC) combined with the recycling of CO2....

The feasibility of synthetic fuels in renewable energy systems

DEFF Research Database (Denmark)

Ridjan, Iva; Mathiesen, Brian Vad; Connolly, David

2012-01-01

supplies, and other impacts on environment and biosphere. Hence, it is essential to make a detailed analysis of this sector in order to match the demand and to meet the criteria of a 100% renewable energy system in 2050. The purpose of this article is to identify potential pathways for producing synthetic...... fuels, with a specific focus on solid oxide electrolyser cells combined with the recycling of CO2....

Future UK markets for stand-alone renewable energy systems

International Nuclear Information System (INIS)

Paish, O.

1999-01-01

A study to identify and quantify the market for stand-alone renewable energy supplies of power (photovoltaics, wind and micro-hydro electricity systems) was described. The study focused on small systems, generally less than a few kW installed capacity. It was suggested that in the UK, the emphasis on grid-connected renewable energy technologies (RETs) has blurred the fact that it is 'off-grid' renewable systems that can offer more immediate real commercial markets for the renewables business. With the likelihood of a significant increase in demand for renewables world wide over the next ten years, the UK needs to make a special effort to become involved

Nrf2 is required to maintain the self-renewal of glioma stem cells

International Nuclear Information System (INIS)

Zhu, Jianhong; Wang, Handong; Sun, Qing; Ji, Xiangjun; Zhu, Lin; Cong, Zixiang; Zhou, Yuan; Liu, Huandong; Zhou, Mengliang

2013-01-01

Glioblastomas are deadly cancers that display a functional cellular hierarchy maintained by self-renewing glioma stem cells (GSCs). Self-renewal is a complex biological process necessary for maintaining the glioma stem cells. Nuclear factor rythroid 2-related factor 2(Nrf2) plays a significant role in protecting cells from endogenous and exogenous stresses. Nrf2 is a key nuclear transcription factor that regulates antioxidant response element (ARE)-containing genes. Previous studies have demonstrated the significant role of Nrf2 in the proliferation of glioblastoma, and in their resistance to radioactive therapies. We examined the effect of knocking down Nrf2 in GSCs. Nrf2 expression was down-regulated by shRNA transinfected with lentivirus. Expression levels of Nestin, Nrf2, BMI-1, Sox2 and Cyclin E were assessed by western blotting, quantitative polymerase chain reaction (qPCR) and immunohistochemistry analysis. The capacity for self-renewal in vitro was assessed by genesis of colonies. The capacity for self-renewal in vivo was analyzed by tumor genesis of xenografts in nude mice. Knockdown of Nrf2 inhibited the proliferation of GSCs, and significantly reduced the expression of BMI-1, Sox2 and CyclinE. Knocking down of Nrf2 changed the cell cycle distribution of GSCs by causing an uncharacteristic increase in the proportion of cells in the G2 phase and a decrease in the proportion of cells in the S phase of the cell cycle. Nrf2 is required to maintain the self-renewal of GSCs, and its down-regulation can attenuate the self-renewal of GSCs significantly

Human mesenchymal stem cells self-renew and differentiate according to a deterministic hierarchy.

Directory of Open Access Journals (Sweden)

Rahul Sarugaser

Full Text Available BACKGROUND: Mesenchymal progenitor cells (MPCs have been isolated from a variety of connective tissues, and are commonly called "mesenchymal stem cells" (MSCs. A stem cell is defined as having robust clonal self-renewal and multilineage differentiation potential. Accordingly, the term "MSC" has been criticised, as there is little data demonstrating self-renewal of definitive single-cell-derived (SCD clonal populations from a mesenchymal cell source. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that a tractable MPC population, human umbilical cord perivascular cells (HUCPVCs, was capable of multilineage differentiation in vitro and, more importantly, contributed to rapid connective tissue healing in vivo by producing bone, cartilage and fibrous stroma. Furthermore, HUCPVCs exhibit a high clonogenic frequency, allowing us to isolate definitive SCD parent and daughter clones from mixed gender suspensions as determined by Y-chromosome fluorescent in situ hybridization. CONCLUSIONS/SIGNIFICANCE: Analysis of the multilineage differentiation capacity of SCD parent clones and daughter clones enabled us to formulate a new hierarchical schema for MSC self-renewal and differentiation in which a self-renewing multipotent MSC gives rise to more restricted self-renewing progenitors that gradually lose differentiation potential until a state of complete restriction to the fibroblast is reached.

Design, analysis, operation, and advanced control of hybrid renewable energy systems

Science.gov (United States)

Whiteman, Zachary S.

Because using non-renewable energy systems (e.g., coal-powered co-generation power plants) to generate electricity is an unsustainable, environmentally hazardous practice, it is important to develop cost-effective and reliable renewable energy systems, such as photovoltaics (PVs), wind turbines (WTs), and fuel cells (FCs). Non-renewable energy systems, however, are currently less expensive than individual renewable energy systems (IRESs). Furthermore, IRESs based on intermittent natural resources (e.g., solar irradiance and wind) are incapable of meeting continuous energy demands. Such shortcomings can be mitigated by judiciously combining two or more complementary IRESs to form a hybrid renewable energy system (HRES). Although previous research efforts focused on the design, operation, and control of HRESs has proven useful, no prior HRES research endeavor has taken a systematic and comprehensive approach towards establishing guidelines by which HRESs should be designed, operated, and controlled. The overall goal of this dissertation, therefore, is to establish the principles governing the design, operation, and control of HRESs resulting in cost-effective and reliable energy solutions for stationary and mobile applications. To achieve this goal, we developed and demonstrated four separate HRES principles. Rational selection of HRES type: HRES components and their sizes should be rationally selected using knowledge of component costs, availability of renewable energy resources, and expected power demands of the application. HRES design: by default, the components of a HRES should be arranged in parallel for increased efficiency and reliability. However, a series HRES design may be preferred depending on the operational considerations of the HRES components. HRES control strategy selection: the choice of HRES control strategy depends on the dynamics of HRES components, their operational considerations, and the practical limitations of the HRES end-use. HRES data

Identifying The Most Applicable Renewable Energy Systems Of Iran

Directory of Open Access Journals (Sweden)

Nasibeh Mousavi

2017-03-01

Full Text Available These years because of energy crisis all of country try to find a new way to reduce energy consumptions and obtain maximum use of renewable energy. Iran also is not an exception of this progress. Renewable energy is energy that is provided by renewable sources such as the sun or wind. In general renewable energies are not adaptable to every single community. Because of location and special climate conditions of Iran most applicable renewable energy systems in Iran are solar and wind energy. Main purpose of this paper is to review and identify most applicable renewable energy systems of Iran and also review on traditional and current methods that utilized to obtain maximum use of these renewable energies.

Transient inhibition of cell proliferation does not compromise self-renewal of mouse embryonic stem cells

Energy Technology Data Exchange (ETDEWEB)

Wang, Ruoxing [Department of Biological Sciences, The University of Southern Mississippi, 118 College Drive 5018, Hattiesburg, MS 39406 (United States); Guo, Yan-Lin, E-mail: yanlin.guo@usm.edu [Department of Biological Sciences, The University of Southern Mississippi, 118 College Drive 5018, Hattiesburg, MS 39406 (United States)

2012-10-01

Embryonic stem cells (ESCs) have unlimited capacity for self-renewal and can differentiate into various cell types when induced. They also have an unusual cell cycle control mechanism driven by constitutively active cyclin dependent kinases (Cdks). In mouse ESCs (mESCs). It is proposed that the rapid cell proliferation could be a necessary part of mechanisms that maintain mESC self-renewal and pluripotency, but this hypothesis is not in line with the finding in human ESCs (hESCs) that the length of the cell cycle is similar to differentiated cells. Therefore, whether rapid cell proliferation is essential for the maintenance of mESC state remains unclear. We provide insight into this uncertainty through chemical intervention of mESC cell cycle. We report here that inhibition of Cdks with olomoucine II can dramatically slow down cell proliferation of mESCs with concurrent down-regulation of cyclin A, B and E, and the activation of the Rb pathway. However, mESCs display can recover upon the removal of olomoucine II and are able to resume normal cell proliferation without losing self-renewal and pluripotency, as demonstrated by the expression of ESC markers, colony formation, embryoid body formation, and induced differentiation. We provide a mechanistic explanation for these observations by demonstrating that Oct4 and Nanog, two major transcription factors that play critical roles in the maintenance of ESC properties, are up-regulated via de novo protein synthesis when the cells are exposed to olomoucine II. Together, our data suggest that short-term inhibition of cell proliferation does not compromise the basic properties of mESCs. -- Highlights: Black-Right-Pointing-Pointer Inhibition of Cdks slows down mESCs proliferation. Black-Right-Pointing-Pointer mESCs display remarkable recovery capacity from short-term cell cycle interruption. Black-Right-Pointing-Pointer Short-term cell cycle interruption does not compromise mESC self-renewal. Black

Transient inhibition of cell proliferation does not compromise self-renewal of mouse embryonic stem cells

International Nuclear Information System (INIS)

Wang, Ruoxing; Guo, Yan-Lin

2012-01-01

Embryonic stem cells (ESCs) have unlimited capacity for self-renewal and can differentiate into various cell types when induced. They also have an unusual cell cycle control mechanism driven by constitutively active cyclin dependent kinases (Cdks). In mouse ESCs (mESCs). It is proposed that the rapid cell proliferation could be a necessary part of mechanisms that maintain mESC self-renewal and pluripotency, but this hypothesis is not in line with the finding in human ESCs (hESCs) that the length of the cell cycle is similar to differentiated cells. Therefore, whether rapid cell proliferation is essential for the maintenance of mESC state remains unclear. We provide insight into this uncertainty through chemical intervention of mESC cell cycle. We report here that inhibition of Cdks with olomoucine II can dramatically slow down cell proliferation of mESCs with concurrent down-regulation of cyclin A, B and E, and the activation of the Rb pathway. However, mESCs display can recover upon the removal of olomoucine II and are able to resume normal cell proliferation without losing self-renewal and pluripotency, as demonstrated by the expression of ESC markers, colony formation, embryoid body formation, and induced differentiation. We provide a mechanistic explanation for these observations by demonstrating that Oct4 and Nanog, two major transcription factors that play critical roles in the maintenance of ESC properties, are up-regulated via de novo protein synthesis when the cells are exposed to olomoucine II. Together, our data suggest that short-term inhibition of cell proliferation does not compromise the basic properties of mESCs. -- Highlights: ► Inhibition of Cdks slows down mESCs proliferation. ► mESCs display remarkable recovery capacity from short-term cell cycle interruption. ► Short-term cell cycle interruption does not compromise mESC self-renewal. ► Oct4 and Nanog are up-regulated via de novo synthesis by cell cycle interruption.

New insights into redox regulation of stem cell self-renewal and differentiation.

Science.gov (United States)

Ren, Fenglian; Wang, Kui; Zhang, Tao; Jiang, Jingwen; Nice, Edouard Collins; Huang, Canhua

2015-08-01

Reactive oxygen species (ROS), the natural byproducts of aerobic metabolism, are precisely orchestrated to evoke diverse signaling pathways. To date, studies have focused mainly on the detrimental effects of ROS in stem cells. Recently, accumulating evidence has suggested that ROS also function as second messengers that modulate stem cell self-renewal and differentiation by regulating intricate signaling networks. Although many efforts have been made to clarify the general effects of ROS on signal transduction in stem cells, less is known about the initial and direct executors of ROS signaling, which are known as 'redox sensors'. Modifications of cysteine residues in redox sensors are of significant importance in the modulation of protein function in response to different redox conditions. Intriguingly, most key molecules in ROS signaling and cell cycle regulation (including transcriptional factors and kinases) that are crucial in the regulation of stem cell self-renewal and differentiation have the potential to be redox sensors. We highlight herein the importance of redox regulation of these key regulators in stem cell self-renewal and differentiation. Understanding the mechanisms of redox regulation in stem cell self-renewal and differentiation will open exciting new perspectives for stem cell biology. This article is part of a Special Issue entitled Redox regulation of differentiation and de-differentiation. Copyright © 2015 Elsevier B.V. All rights reserved.

SCL, LMO1 and Notch1 Reprogram Thymocytes into Self-Renewing Cells

Science.gov (United States)

Rojas-Sutterlin, Shanti; Herblot, Sabine; Hébert, Josée; Sauvageau, Guy; Lemieux, Sébastien; Lécuyer, Eric; Veiga, Diogo F. T.; Hoang, Trang

2014-01-01

The molecular determinants that render specific populations of normal cells susceptible to oncogenic reprogramming into self-renewing cancer stem cells are poorly understood. Here, we exploit T-cell acute lymphoblastic leukemia (T-ALL) as a model to define the critical initiating events in this disease. First, thymocytes that are reprogrammed by the SCL and LMO1 oncogenic transcription factors into self-renewing pre-leukemic stem cells (pre-LSCs) remain non-malignant, as evidenced by their capacities to generate functional T cells. Second, we provide strong genetic evidence that SCL directly interacts with LMO1 to activate the transcription of a self-renewal program coordinated by LYL1. Moreover, LYL1 can substitute for SCL to reprogram thymocytes in concert with LMO1. In contrast, inhibition of E2A was not sufficient to substitute for SCL, indicating that thymocyte reprogramming requires transcription activation by SCL-LMO1. Third, only a specific subset of normal thymic cells, known as DN3 thymocytes, is susceptible to reprogramming. This is because physiological NOTCH1 signals are highest in DN3 cells compared to other thymocyte subsets. Consistent with this, overexpression of a ligand-independent hyperactive NOTCH1 allele in all immature thymocytes is sufficient to sensitize them to SCL-LMO1, thereby increasing the pool of self-renewing cells. Surprisingly, hyperactive NOTCH1 cannot reprogram thymocytes on its own, despite the fact that NOTCH1 is activated by gain of function mutations in more than 55% of T-ALL cases. Rather, elevating NOTCH1 triggers a parallel pathway involving Hes1 and Myc that dramatically enhances the activity of SCL-LMO1 We conclude that the acquisition of self-renewal and the genesis of pre-LSCs from thymocytes with a finite lifespan represent a critical first event in T-ALL. Finally, LYL1 and LMO1 or LMO2 are co-expressed in most human T-ALL samples, except the cortical T subtype. We therefore anticipate that the self-renewal network

Polycomb Cbx family members mediate the balance between haematopoietic stem cell self-renewal and differentiation

DEFF Research Database (Denmark)

Klauke, Karin; Radulović, Višnja; Broekhuis, Mathilde

2013-01-01

The balance between self-renewal and differentiation of adult stem cells is essential for tissue homeostasis. Here we show that in the haematopoietic system this process is governed by polycomb chromobox (Cbx) proteins. Cbx7 is specifically expressed in haematopoietic stem cells (HSCs), and its...... overexpression enhances self-renewal and induces leukaemia. This effect is dependent on integration into polycomb repressive complex-1 (PRC1) and requires H3K27me3 binding. In contrast, overexpression of Cbx2, Cbx4 or Cbx8 results in differentiation and exhaustion of HSCs. ChIP-sequencing analysis shows that Cbx......7 and Cbx8 share most of their targets; we identified approximately 200 differential targets. Whereas genes targeted by Cbx8 are highly expressed in HSCs and become repressed in progenitors, Cbx7 targets show the opposite expression pattern. Thus, Cbx7 preserves HSC self-renewal by repressing...

Tissue-resident adult stem cell populations of rapidly self-renewing organs

NARCIS (Netherlands)

Barker, N.; Bartfeld, S.; Clevers, H.

2010-01-01

The epithelial lining of the intestine, stomach, and skin is continuously exposed to environmental assault, imposing a requirement for regular self-renewal. Resident adult stem cell populations drive this renewal, and much effort has been invested in revealing their identity. Reliable adult stem

Study of renewable energy, fuel cell and demotics integration for stationary energy production

Energy Technology Data Exchange (ETDEWEB)

Andaloro, L.; Ferraro, M.; Sergi, F.; Brunaccini, G.; Antonucci, V. [National Research Inst., Messina (Italy)

2009-07-01

This paper described a study in which a small house equipped with various renewable technologies was modelled. The aim of the study was to evaluated the integration of fuel cells with various other energy sources. Technologies installed in the house included a photovoltaic (PV) system; a hydrogen system; fuel cells; a battery-storage system; and a thermal solar panel. Maximum energy savings were evaluated for different configurations and combinations of the installed energy sources. A domotic system was also used to automatically control the use of electrical appliances and improve safety and comfort. An energy side management system was designed and compared with a demand side management system. Various scenarios were simulated in order to test the energy management systems in relation to the automated domotic system.

Identification of key factors regulating self-renewal and differentiation in EML hematopoietic precursor cells by RNA-sequencing analysis.

Science.gov (United States)

Zong, Shan; Deng, Shuyun; Chen, Kenian; Wu, Jia Qian

2014-11-11

Hematopoietic stem cells (HSCs) are used clinically for transplantation treatment to rebuild a patient's hematopoietic system in many diseases such as leukemia and lymphoma. Elucidating the mechanisms controlling HSCs self-renewal and differentiation is important for application of HSCs for research and clinical uses. However, it is not possible to obtain large quantity of HSCs due to their inability to proliferate in vitro. To overcome this hurdle, we used a mouse bone marrow derived cell line, the EML (Erythroid, Myeloid, and Lymphocytic) cell line, as a model system for this study. RNA-sequencing (RNA-Seq) has been increasingly used to replace microarray for gene expression studies. We report here a detailed method of using RNA-Seq technology to investigate the potential key factors in regulation of EML cell self-renewal and differentiation. The protocol provided in this paper is divided into three parts. The first part explains how to culture EML cells and separate Lin-CD34+ and Lin-CD34- cells. The second part of the protocol offers detailed procedures for total RNA preparation and the subsequent library construction for high-throughput sequencing. The last part describes the method for RNA-Seq data analysis and explains how to use the data to identify differentially expressed transcription factors between Lin-CD34+ and Lin-CD34- cells. The most significantly differentially expressed transcription factors were identified to be the potential key regulators controlling EML cell self-renewal and differentiation. In the discussion section of this paper, we highlight the key steps for successful performance of this experiment. In summary, this paper offers a method of using RNA-Seq technology to identify potential regulators of self-renewal and differentiation in EML cells. The key factors identified are subjected to downstream functional analysis in vitro and in vivo.

Solid Oxide Fuel Cells Operating on Alternative and Renewable Fuels

Energy Technology Data Exchange (ETDEWEB)

Wang, Xiaoxing; Quan, Wenying; Xiao, Jing; Peduzzi, Emanuela; Fujii, Mamoru; Sun, Funxia; Shalaby, Cigdem; Li, Yan; Xie, Chao; Ma, Xiaoliang; Johnson, David; Lee, Jeong; Fedkin, Mark; LaBarbera, Mark; Das, Debanjan; Thompson, David; Lvov, Serguei; Song, Chunshan

2014-09-30

This DOE project at the Pennsylvania State University (Penn State) initially involved Siemens Energy, Inc. to (1) develop new fuel processing approaches for using selected alternative and renewable fuels – anaerobic digester gas (ADG) and commercial diesel fuel (with 15 ppm sulfur) – in solid oxide fuel cell (SOFC) power generation systems; and (2) conduct integrated fuel processor – SOFC system tests to evaluate the performance of the fuel processors and overall systems. Siemens Energy Inc. was to provide SOFC system to Penn State for testing. The Siemens work was carried out at Siemens Energy Inc. in Pittsburgh, PA. The unexpected restructuring in Siemens organization, however, led to the elimination of the Siemens Stationary Fuel Cell Division within the company. Unfortunately, this led to the Siemens subcontract with Penn State ending on September 23rd, 2010. SOFC system was never delivered to Penn State. With the assistance of NETL project manager, the Penn State team has since developed a collaborative research with Delphi as the new subcontractor and this work involved the testing of a stack of planar solid oxide fuel cells from Delphi.

CD47 regulates renal tubular epithelial cell self-renewal and proliferation following renal ischemia reperfusion.

Science.gov (United States)

Rogers, Natasha M; Zhang, Zheng J; Wang, Jiao-Jing; Thomson, Angus W; Isenberg, Jeffrey S

2016-08-01

Defects in renal tubular epithelial cell repair contribute to renal ischemia reperfusion injury, cause acute kidney damage, and promote chronic renal disease. The matricellular protein thrombospondin-1 and its receptor CD47 are involved in experimental renal ischemia reperfusion injury, although the role of this interaction in renal recovery is unknown. We found upregulation of self-renewal genes (transcription factors Oct4, Sox2, Klf4 and cMyc) in the kidney of CD47(-/-) mice after ischemia reperfusion injury. Wild-type animals had minimal self-renewal gene expression, both before and after injury. Suggestive of cell autonomy, CD47(-/-) renal tubular epithelial cells were found to increase expression of the self-renewal genes. This correlated with enhanced proliferative capacity compared with cells from wild-type mice. Exogenous thrombospondin-1 inhibited self-renewal gene expression in renal tubular epithelial cells from wild-type but not CD47(-/-) mice, and this was associated with decreased proliferation. Treatment of renal tubular epithelial cells with a CD47 blocking antibody or CD47-targeting small interfering RNA increased expression of some self-renewal transcription factors and promoted cell proliferation. In a syngeneic kidney transplant model, treatment with a CD47 blocking antibody increased self-renewal transcription factor expression, decreased tissue damage, and improved renal function compared with that in control mice. Thus, thrombospondin-1 via CD47 inhibits renal tubular epithelial cell recovery after ischemia reperfusion injury through inhibition of proliferation/self-renewal. Copyright © 2016 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.

Spermatogonial stem cell renewal following irradiation

International Nuclear Information System (INIS)

Fabrikant, J.I.

1979-05-01

The spermatogonial cell renewal system can maintain function and a steady level of cell population for relatively long periods of continuous low-level irradiation indicating that there does not appear to be a serious accumulation, over many generations, of damage affecting proliferation. Provided the dose-rate is quite low, there is an effective selective removal of damaged cells with almost complete repair of cellular nonlethal damage. At dose-rates greater than 2 rad/day, spermatogonia are very sensitive to radiation death, and the main reason for the low tolerance to continuous stress could, in part, be the limited extent of compensatory mechanisms regulating spermatogonial cell production. However, there is some capacity to change the patterns of cellular proliferation while still remaining under homeostatic control, and this capacity appears to reside in the relatively radioresistant A/sub s/ stem-cell population. Little is known about the extent to which the spermatogonial cell population can repair nonlethal cellular radiation damage accumulated under continuous stress affecting the regenerative capacity of the tissue. After acute exposure, a minimum number of surviving type A/sub s/ stem-cells are required to repopulate the functional seminiferous epithelium, regeneration proceeds along an ordered cell stage sequence, and is dependent on the time required for all stages from type A/sub s/ spermatogonia to mature spermatozoa. Under continuous irradiation, provided the dose-rate is not too high, the repopulating ability of the seminiferous epithelium is maintained, in the presence of injury, due to initial repair and long-term repair of cellular radiation damage. There is evidence for initial repair, since a dose-rate effect exists in type A survival, at low doses. Long-term repair occurs due to differential radiosensitivities of spermatogonia

Nuclear Energy and Renewables interaction: System Effects in Low-carbon Electricity Systems

International Nuclear Information System (INIS)

Keppler, Jan Horst; Cometto, Marco

2013-01-01

This report presents a synthesis of the OECD/NEA study 'Nuclear Energy and Renewables: System Effects in Low-carbon Electricity Systems'. It addresses the increasingly important interactions of variable renewables and dispatchable energy technologies, such as nuclear power, in terms of their effects on electricity systems. These effects add costs to the production of electricity, which are not usually transparent. The report recommends that decision-makers should take into account such system costs and internalise them according to a 'generator pays' principle, which is currently not the case. Analysing data from six OECD/NEA countries, the study finds that including the system costs of variable renewables at the level of the electricity grid increases the total costs of electricity supply by up to one-third, depending on technology, country and penetration levels. In addition, it concludes that, unless the current market subsidies for renewables are altered, dispatchable technologies will increasingly not be replaced as they reach their end of life and consequently security of supply will suffer. This implies that significant changes in management and cost allocation will be needed to generate the flexibility required for an economically viable coexistence of nuclear energy and renewables in increasingly de-carbonised electricity systems

Backup flexibility classes in emerging large-scale renewable electricity systems

International Nuclear Information System (INIS)

Schlachtberger, D.P.; Becker, S.; Schramm, S.; Greiner, M.

2016-01-01

Highlights: • Flexible backup demand in a European wind and solar based power system is modelled. • Three flexibility classes are defined based on production and consumption timescales. • Seasonal backup capacities are shown to be only used below 50% renewable penetration. • Large-scale transmission between countries can reduce fast flexible capacities. - Abstract: High shares of intermittent renewable power generation in a European electricity system will require flexible backup power generation on the dominant diurnal, synoptic, and seasonal weather timescales. The same three timescales are already covered by today’s dispatchable electricity generation facilities, which are able to follow the typical load variations on the intra-day, intra-week, and seasonal timescales. This work aims to quantify the changing demand for those three backup flexibility classes in emerging large-scale electricity systems, as they transform from low to high shares of variable renewable power generation. A weather-driven modelling is used, which aggregates eight years of wind and solar power generation data as well as load data over Germany and Europe, and splits the backup system required to cover the residual load into three flexibility classes distinguished by their respective maximum rates of change of power output. This modelling shows that the slowly flexible backup system is dominant at low renewable shares, but its optimized capacity decreases and drops close to zero once the average renewable power generation exceeds 50% of the mean load. The medium flexible backup capacities increase for modest renewable shares, peak at around a 40% renewable share, and then continuously decrease to almost zero once the average renewable power generation becomes larger than 100% of the mean load. The dispatch capacity of the highly flexible backup system becomes dominant for renewable shares beyond 50%, and reach their maximum around a 70% renewable share. For renewable shares

Nanog regulates self-renewal of cancer stem cells through the insulin-like growth factor pathway in human hepatocellular carcinoma.

Science.gov (United States)

Shan, Juanjuan; Shen, Junjie; Liu, Limei; Xia, Feng; Xu, Chuan; Duan, Guangjie; Xu, Yanmin; Ma, Qinghua; Yang, Zhi; Zhang, Qianzhen; Ma, Leina; Liu, Jia; Xu, Senlin; Yan, Xiaochu; Bie, Ping; Cui, Youhong; Bian, Xiu-wu; Qian, Cheng

2012-09-01

Hepatocellular carcinoma (HCC) exhibits cellular heterogeneity and embryonic stem-cell-related genes are preferentially overexpressed in a fraction of cancer cells of poorly differentiated tumors. However, it is not known whether or how these cancer cells contribute to tumor initiation and progression. Here, our data showed that increased expression of pluripotency transcription factor Nanog in cancer cells correlates with a worse clinical outcome in HCC. Using the Nanog promoter as a reporter system, we could successfully isolate a small subpopulation of Nanog-positive cells. We demonstrate that Nanog-positive cells exhibited enhanced ability of self-renewal, clonogenicity, and initiation of tumors, which are consistent with crucial hallmarks in the definition of cancer stem cells (CSCs). Nanog(Pos) CSCs could differentiate into mature cancer cells in in vitro and in vivo conditions. In addition, we found that Nanog(Pos) CSCs exhibited resistance to therapeutic agents (e.g., sorafenib and cisplatin) and have a high capacity for tumor invasion and metastasis. Knock-down expression of Nanog in Nanog(Pos) CSCs could decrease self-renewal accompanied with decreased expression of stem-cell-related genes and increased expression of mature hepatocyte-related genes. Overexpression of Nanog in Nanog(Neg) cells could restore self-renewal. Furthermore, we found that insulin-like growth factor (IGF)2 and IGF receptor (IGF1R) were up-regulated in Nanog(Pos) CSCs. Knock-down expression of Nanog in Nanog(Pos) CSCs inhibited the expression of IGF1R, and overexpression of Nanog in Nanog(Neg) cells increased the expression of IGF1R. A specific inhibitor of IGF1R signaling could significantly inhibit self-renewal and Nanog expression, indicating that IGF1R signaling participated in Nanog-mediated self-renewal. These data indicate that Nanog could be a novel biomarker for CSCs in HCC, and that Nanog could play a crucial role in maintaining the self-renewal of CSCs through the IGF1R

Protein kinase C regulates human pluripotent stem cell self-renewal.

Directory of Open Access Journals (Sweden)

Masaki Kinehara

Full Text Available The self-renewal of human pluripotent stem (hPS cells including embryonic stem and induced pluripotent stem cells have been reported to be supported by various signal pathways. Among them, fibroblast growth factor-2 (FGF-2 appears indispensable to maintain self-renewal of hPS cells. However, downstream signaling of FGF-2 has not yet been clearly understood in hPS cells.In this study, we screened a kinase inhibitor library using a high-throughput alkaline phosphatase (ALP activity-based assay in a minimal growth factor-defined medium to understand FGF-2-related molecular mechanisms regulating self-renewal of hPS cells. We found that in the presence of FGF-2, an inhibitor of protein kinase C (PKC, GF109203X (GFX, increased ALP activity. GFX inhibited FGF-2-induced phosphorylation of glycogen synthase kinase-3β (GSK-3β, suggesting that FGF-2 induced PKC and then PKC inhibited the activity of GSK-3β. Addition of activin A increased phosphorylation of GSK-3β and extracellular signal-regulated kinase-1/2 (ERK-1/2 synergistically with FGF-2 whereas activin A alone did not. GFX negated differentiation of hPS cells induced by the PKC activator, phorbol 12-myristate 13-acetate whereas Gö6976, a selective inhibitor of PKCα, β, and γ isoforms could not counteract the effect of PMA. Intriguingly, functional gene analysis by RNA interference revealed that the phosphorylation of GSK-3β was reduced by siRNA of PKCδ, PKCε, and ζ, the phosphorylation of ERK-1/2 was reduced by siRNA of PKCε and ζ, and the phosphorylation of AKT was reduced by PKCε in hPS cells.Our study suggested complicated cross-talk in hPS cells that FGF-2 induced the phosphorylation of phosphatidylinositol-3 kinase (PI3K/AKT, mitogen-activated protein kinase/ERK-1/2 kinase (MEK, PKC/ERK-1/2 kinase, and PKC/GSK-3β. Addition of GFX with a MEK inhibitor, U0126, in the presence of FGF-2 and activin A provided a long-term stable undifferentiated state of hPS cells even though h

Protein Kinase C Regulates Human Pluripotent Stem Cell Self-Renewal

Science.gov (United States)

Kinehara, Masaki; Kawamura, Suguru; Tateyama, Daiki; Suga, Mika; Matsumura, Hiroko; Mimura, Sumiyo; Hirayama, Noriko; Hirata, Mitsuhi; Uchio-Yamada, Kozue; Kohara, Arihiro; Yanagihara, Kana; Furue, Miho K.

2013-01-01

Background The self-renewal of human pluripotent stem (hPS) cells including embryonic stem and induced pluripotent stem cells have been reported to be supported by various signal pathways. Among them, fibroblast growth factor-2 (FGF-2) appears indispensable to maintain self-renewal of hPS cells. However, downstream signaling of FGF-2 has not yet been clearly understood in hPS cells. Methodology/Principal Findings In this study, we screened a kinase inhibitor library using a high-throughput alkaline phosphatase (ALP) activity-based assay in a minimal growth factor-defined medium to understand FGF-2-related molecular mechanisms regulating self-renewal of hPS cells. We found that in the presence of FGF-2, an inhibitor of protein kinase C (PKC), GF109203X (GFX), increased ALP activity. GFX inhibited FGF-2-induced phosphorylation of glycogen synthase kinase-3β (GSK-3β), suggesting that FGF-2 induced PKC and then PKC inhibited the activity of GSK-3β. Addition of activin A increased phosphorylation of GSK-3β and extracellular signal-regulated kinase-1/2 (ERK-1/2) synergistically with FGF-2 whereas activin A alone did not. GFX negated differentiation of hPS cells induced by the PKC activator, phorbol 12-myristate 13-acetate whereas Gö6976, a selective inhibitor of PKCα, β, and γ isoforms could not counteract the effect of PMA. Intriguingly, functional gene analysis by RNA interference revealed that the phosphorylation of GSK-3β was reduced by siRNA of PKCδ, PKCε, and ζ, the phosphorylation of ERK-1/2 was reduced by siRNA of PKCε and ζ, and the phosphorylation of AKT was reduced by PKCε in hPS cells. Conclusions/Significance Our study suggested complicated cross-talk in hPS cells that FGF-2 induced the phosphorylation of phosphatidylinositol-3 kinase (PI3K)/AKT, mitogen-activated protein kinase/ERK-1/2 kinase (MEK), PKC/ERK-1/2 kinase, and PKC/GSK-3β. Addition of GFX with a MEK inhibitor, U0126, in the presence of FGF-2 and activin A provided a long

Involvement of extracellular factors in maintaining self-renewal of neural stem cell by nestin.

Science.gov (United States)

Di, Chun Guang; Xiang, Andy Peng; Jia, Lei; Liu, Jun Feng; Lahn, Bruce T; Ma, Bao Feng

2014-07-09

Nestin knockout leads to embryonic lethality and self-renewal deficiency in neural stem cells (NSCs). However, how nestin maintains self-renewal remains uncertain. Here, we used the dosage effect of nestin in heterozygous mice (Nes+/-) to study self-renewal of NSCs. With existing extracellular signaling in vivo or in vitro, nestin levels do not affect proliferation ability or apoptosis when compared between Nes+/- and Nes+/+ NSCs. However, self-renewal ability of Nes+/- NSCs is impaired when plated at a low cell density and completely lost at a clonal density. This deficiency in self-renewal at a clonal density is rescued using a medium conditioned by Nes+/+ NSCs. In addition, the Akt signaling pathway is altered at low density and reversed by conditioned medium. Our data show that secreted factors contribute toward maintaining self-renewal of NSCs by nestin, potentially through Akt signaling.

The first step towards a 100% renewable energy-system for Ireland

International Nuclear Information System (INIS)

Connolly, D.; Leahy, M.; Lund, H.; Mathiesen, B.V.

2011-01-01

In 2007 Ireland supplied 96% of the total energy demand with fossil fuels (7% domestic and 89% imported) and 3% with renewable energy, even though there are enough renewable resources to supply all the energy required. As energy prices increase and the effects of global warming worsen, it is essential that Ireland begins to utilise its renewable resources more effectively. Therefore, this study presents the first step towards a 100% renewable energy-system for Ireland. The energy-system analysis tool used was EnergyPLAN, as it accounts for all sectors of the energy-system that need to be considered when integrating large penetrations of renewable energy: the electricity, heat, and transport sectors. Initially, a reference model of the existing Irish energy-system was constructed, and subsequently three different 100% renewable energy-systems were created with each focusing on a different resource: biomass, hydrogen, and electricity. These energy-systems were compared so that the benefits from each could be used to create an 'optimum' scenario called combination. Although the results illustrate a potential 100% renewable energy-system for Ireland, they have been obtained based on numerous assumptions. Therefore, these will need to be improved in the future before a serious roadmap can be defined for Ireland's renewable energy transition. (author)

The role of district heating in future renewable energy systems

DEFF Research Database (Denmark)

Lund, Henrik; Möller, Bernd; Mathiesen, Brian Vad

2010-01-01

Based on the case of Denmark, this paper analyses the role of district heating in future Renewable Energy Systems. At present, the share of renewable energy is coming close to 20 per cent. From such point of departure, the paper defines a scenario framework in which the Danish system is converted...... to 100 per cent Renewable Energy Sources (RES) in the year 2060 including reductions in space heating demands by 75 per cent. By use of a detailed energy system analysis of the complete national energy system, the consequences in relation to fuel demand, CO2 emissions and cost are calculated for various...... as in a potential future system based 100 per cent on renewable energy....

Transient inhibition of cell proliferation does not compromise self-renewal of mouse embryonic stem cells.

Science.gov (United States)

Wang, Ruoxing; Guo, Yan-Lin

2012-10-01

Embryonic stem cells (ESCs) have unlimited capacity for self-renewal and can differentiate into various cell types when induced. They also have an unusual cell cycle control mechanism driven by constitutively active cyclin dependent kinases (Cdks). In mouse ESCs (mESCs). It is proposed that the rapid cell proliferation could be a necessary part of mechanisms that maintain mESC self-renewal and pluripotency, but this hypothesis is not in line with the finding in human ESCs (hESCs) that the length of the cell cycle is similar to differentiated cells. Therefore, whether rapid cell proliferation is essential for the maintenance of mESC state remains unclear. We provide insight into this uncertainty through chemical intervention of mESC cell cycle. We report here that inhibition of Cdks with olomoucine II can dramatically slow down cell proliferation of mESCs with concurrent down-regulation of cyclin A, B and E, and the activation of the Rb pathway. However, mESCs display can recover upon the removal of olomoucine II and are able to resume normal cell proliferation without losing self-renewal and pluripotency, as demonstrated by the expression of ESC markers, colony formation, embryoid body formation, and induced differentiation. We provide a mechanistic explanation for these observations by demonstrating that Oct4 and Nanog, two major transcription factors that play critical roles in the maintenance of ESC properties, are up-regulated via de novo protein synthesis when the cells are exposed to olomoucine II. Together, our data suggest that short-term inhibition of cell proliferation does not compromise the basic properties of mESCs. Copyright © 2012 Elsevier Inc. All rights reserved.

Implementation of Renewable Energy Systems in Denmark

DEFF Research Database (Denmark)

Meyer, Niels I

1997-01-01

Denmark has been one of the first countries in the world to commit itself to a sustainable energy development. This has been substantiated by two official action plans from 1990 and 1996 with emphasis on energy efficiency and supply systems based on renewable energy. In year 2005, renewable energy...... sources are planned to cover 12-14% and in year 2030 about 35% of total Danish energy demand. This paper reviews the experiences with implementation of renewable energy in Denmark with a focus on wind power and biomass....

Heating technologies for limiting biomass consumption in 100% renewable energy systems

DEFF Research Database (Denmark)

Mathiesen, Brian Vad; Lund, Henrik; Connolly, David

2011-01-01

district heating enables the use of combined heat and power production (CPH) and other renewable resources than biomass such as large-scale solar thermal, large-heat pumps, geothermal heat, industrial surplus heat etc. which is important for reducing the biomass consumption. Where the energy density......The utilisation of biomass poses large challenges in renewable energy systems and buildings account for a substantial part of the energy supply also in 100% renewable energy systems. The analyses of heating technologies show that district heating systems are especially important in limiting...... the dependence on biomass resources and to create cost effective systems. District heating systems are especially important in renewable energy systems with large amounts of fluctuating renewable energy sources as it enables fuel efficient and lower cost energy systems with thermal heat storages. And also...

Economic dispatch optimization for system integrating renewable energy sources

Science.gov (United States)

Jihane, Kartite; Mohamed, Cherkaoui

2018-05-01

Nowadays, the use of energy is growing especially in transportation and electricity industries. However this energy is based on conventional sources which pollute the environment. Multi-source system is seen as the best solution to sustainable development. This paper proposes the Economic Dispatch (ED) of hybrid renewable power system. The hybrid system is composed of ten thermal generators, photovoltaic (PV) generator and wind turbine generator. To show the importance of renewable energy sources (RES) in the energy mix we have ran the simulation for system integrated PV only and PV plus wind. The result shows that the system with renewable energy sources (RES) is more compromising than the system without RES in terms of fuel cost.

p38 MAPK pathway is essential for self-renewal of mouse male germline stem cells (mGSCs).

Science.gov (United States)

Niu, Zhiwei; Mu, Hailong; Zhu, Haijing; Wu, Jiang; Hua, Jinlian

2017-02-01

Male germline stem cells (mGSCs), also called spermatogonial stem cells (SSCs), constantly generate spermatozoa in male animals. A number of preliminary studies on mechanisms of mGSC self-renewal have previously been conducted, revealing that several factors are involved in this regulated process. The p38 MAPK pathway is widely conserved in multiple cell types in vivo, and plays an important role in cell proliferation, differentiation, inflammation and apoptosis. However, its role in self-renewal of mGSCs has not hitherto been determined. Here, the mouse mGSCs were cultured and their identity was verified by semi-RT-PCR, alkaline phosphatase (AP) staining and immunofluorescence staining. Then, the p38 MAPK pathway was blocked by p38 MAPK-specific inhibitor SB202190. mGSC self-renewal ability was then analysed by observation of morphology, cell number, cell growth analysis, TUNEL incorporation assay and cell cycle analysis. Results showed that mouse mGSC self-renewal ability was significantly inhibited by SB202190. This study showed for the first time that the p38 MAPK pathway plays a key role in maintaining self-renewal capacity of mouse mGSCs, which offers a new self-renewal pathway for these cells and contributes to overall knowledge of the mechanisms of mGSC self-renewal. © 2016 John Wiley & Sons Ltd.

Power electronics for renewable energy systems

DEFF Research Database (Denmark)

Iov, Florin; Blaabjerg, Frede

2009-01-01

sources from the conventional, fossil (and short term) based energy sources to renewable energy resources. Another is to use high efficient power electronics in power generation, power transmission/distribution and end-user application. This paper discuss some of the most emerging renewable energy sources......, wind energy and photovoltaics, which by means of power electronics are changing from being minor energy sources to be acting as important power sources in the energy system....

Sustainability index approach as a selection criteria for energy storage system of an intermittent renewable energy source

International Nuclear Information System (INIS)

Raza, Syed Shabbar; Janajreh, Isam; Ghenai, Chaouki

2014-01-01

Highlights: • Three renewable energy storage options considered: lead acid and lithium polymer batteries and fuel cell. • Hydrogen fuel cell system is the most feasible energy storage option for the long term energy storage. • Sustainability index approach is a novel method used to quantify the qualitative properties of the system. - Abstract: The sustainability index is an adaptive, multicriteria and novel technique that is used to compare different energy storage systems for their sustainability. This innovative concept utilizes both qualitative and quantitative results to measure sustainability through an index based approach. This report aims to compare three different energy storage options for an intermittent renewable energy source. The three energy storage options are lead acid batteries, lithium polymer batteries and fuel cell systems, that are selected due to their availability and the geographical constrain of using other energy storage options. The renewable energy source used is solar photovoltaic (PV). Several technical, economic and environmental factors have been discussed elaborately which would help us to evaluate the merits of the energy storage system for long term storage. Finally, a novel sustainability index has been proposed which quantifies the qualitative and quantitative aspects of the factors discussed, and thus helps us choose the ideal energy storage system for our scenario. A weighted sum approach is used to quantify each factor according to their importance. After a detailed analysis of the three energy storage systems through the sustainability index approach, the most feasible energy storage option was found to be fuel cell systems which can provide a long term energy storage option and also environmental friendly

Cross-border versus cross-sector interconnectivity in renewable energy systems

DEFF Research Database (Denmark)

Thellufsen, Jakob Zinck; Lund, Henrik

2017-01-01

. The results show that while both measures increase the system utilisation of renewable energy and the system efficiency, the cross-sector interconnection gives the best system performance. To analyse the possible interaction between cross-sector and cross-border interconnectivity, two main aspects have......In the transition to renewable energy systems, fluctuating renewable energy, such as wind and solar power, plays a large and important role. This creates a challenge in terms of meeting demands, as the energy production fluctuates based on weather patterns. To utilise high amounts of fluctuating...... renewable energy, the energy system has to be more flexible in terms of decoupling demand and production. This paper investigates two potential ways to increase flexibility. The first is the interconnection between energy systems, for instance between two countries, labelled as cross-border interconnection...

Depletion of Tcf3 and Lef1 maintains mouse embryonic stem cell self-renewal

OpenAIRE

Ye, Shoudong; Zhang, Tao; Tong, Chang; Zhou, Xingliang; He, Kan; Ban, Qian; Liu, Dahai; Ying, Qi-Long

2017-01-01

ABSTRACT Mouse and rat embryonic stem cell (ESC) self-renewal can be maintained by dual inhibition of glycogen synthase kinase 3 (GSK3) and mitogen-activated protein kinase kinase (MEK). Inhibition of GSK3 promotes ESC self-renewal by abrogating T-cell factor 3 (TCF3)-mediated repression of the pluripotency network. How inhibition of MEK mediates ESC self-renewal, however, remains largely unknown. Here, we show that inhibition of MEK can significantly suppress lymphoid enhancer factor 1 (LEF1...

Limiting biomass consumption for heating in 100% renewable energy systems

DEFF Research Database (Denmark)

Mathiesen, Brian Vad; Lund, Henrik; Connolly, David

2012-01-01

-scale solar thermal, large heat pumps, geothermal heat, industrial surplus heat, and waste incineration. Where the energy density in the building stock is not high enough for DH to be economical, geothermal heat pumps can be recommended for individual heating systems, even though biomass consumption is higher......The utilisation of biomass poses large challenges in renewable energy systems while buildings account for a substantial part of the energy supply even in 100% renewable energy systems. In this paper the focus is on how the heating sector can reduce its consumption of biomass, thus leaving biomass...... for other sectors, but while still enabling a 100% renewable energy system. The analyses of heating technologies shows that district heating (DH) systems are important in limiting the dependence on biomass and create cost effective solutions. DH systems are especially important in renewable energy systems...

Integrating renewables into energy systems

International Nuclear Information System (INIS)

1999-03-01

An analysis of renewable energy schemes was undertaken via case studies in China, India, Indonesia, Kenya, South Africa, Thailand and Zimbabwe, that provided an insight into the application of best practice for overcoming market, technical and financial barriers to the establishment of the sustainable markets required for the large-scale deployment of renewable energy technologies. The project showed clearly the need to select and target interventions according to the context. Lessons were extracted against a number of themes, as well as against the various technologies analysed and simple guides to the principles of best practice were derived under the following headings:- experience of gaining access to (micro) finance; the technical and non-technical issues raised when small, typically independent, generators seek access to central electricity grid systems; how to best undertake awareness raising and dissemination activities; promoting, building and operating biogas systems; promoting, building and operating solar (photovoltaic) home systems; promoting, building and operating grid connected wind power; promoting, building and operating solar hot water systems; promoting agricultural cogeneration using crop residues. (author)

Aubergine Controls Germline Stem Cell Self-Renewal and Progeny Differentiation via Distinct Mechanisms.

Science.gov (United States)

Ma, Xing; Zhu, Xiujuan; Han, Yingying; Story, Benjamin; Do, Trieu; Song, Xiaoqing; Wang, Su; Zhang, Ying; Blanchette, Marco; Gogol, Madelaine; Hall, Kate; Peak, Allison; Anoja, Perera; Xie, Ting

2017-04-24

Piwi family protein Aubergine (Aub) maintains genome integrity in late germ cells of the Drosophila ovary through Piwi-associated RNA-mediated repression of transposon activities. Although it is highly expressed in germline stem cells (GSCs) and early progeny, it remains unclear whether it plays any roles in early GSC lineage development. Here we report that Aub promotes GSC self-renewal and GSC progeny differentiation. RNA-iCLIP results show that Aub binds the mRNAs encoding self-renewal and differentiation factors in cultured GSCs. Aub controls GSC self-renewal by preventing DNA-damage-induced Chk2 activation and by translationally controlling the expression of self-renewal factors. It promotes GSC progeny differentiation by translationally controlling the expression of differentiation factors, including Bam. Therefore, this study reveals a function of Aub in GSCs and their progeny, which promotes translation of self-renewal and differentiation factors by directly binding to its target mRNAs and interacting with translational initiation factors. Copyright © 2017 Elsevier Inc. All rights reserved.

Cellular automata and integrodifferential equation models for cell renewal in mosaic tissues

Science.gov (United States)

Bloomfield, J. M.; Sherratt, J. A.; Painter, K. J.; Landini, G.

2010-01-01

Mosaic tissues are composed of two or more genetically distinct cell types. They occur naturally, and are also a useful experimental method for exploring tissue growth and maintenance. By marking the different cell types, one can study the patterns formed by proliferation, renewal and migration. Here, we present mathematical modelling suggesting that small changes in the type of interaction that cells have with their local cellular environment can lead to very different outcomes for the composition of mosaics. In cell renewal, proliferation of each cell type may depend linearly or nonlinearly on the local proportion of cells of that type, and these two possibilities produce very different patterns. We study two variations of a cellular automaton model based on simple rules for renewal. We then propose an integrodifferential equation model, and again consider two different forms of cellular interaction. The results of the continuous and cellular automata models are qualitatively the same, and we observe that changes in local environment interaction affect the dynamics for both. Furthermore, we demonstrate that the models reproduce some of the patterns seen in actual mosaic tissues. In particular, our results suggest that the differing patterns seen in organ parenchymas may be driven purely by the process of cell replacement under different interaction scenarios. PMID:20375040

Model Scaling of Hydrokinetic Ocean Renewable Energy Systems

Science.gov (United States)

von Ellenrieder, Karl; Valentine, William

2013-11-01

Numerical simulations are performed to validate a non-dimensional dynamic scaling procedure that can be applied to subsurface and deeply moored systems, such as hydrokinetic ocean renewable energy devices. The prototype systems are moored in water 400 m deep and include: subsurface spherical buoys moored in a shear current and excited by waves; an ocean current turbine excited by waves; and a deeply submerged spherical buoy in a shear current excited by strong current fluctuations. The corresponding model systems, which are scaled based on relative water depths of 10 m and 40 m, are also studied. For each case examined, the response of the model system closely matches the scaled response of the corresponding full-sized prototype system. The results suggest that laboratory-scale testing of complete ocean current renewable energy systems moored in a current is possible. This work was supported by the U.S. Southeast National Marine Renewable Energy Center (SNMREC).

Optimizing the U.S. Electric System with a High Penetration of Renewables

Science.gov (United States)

Corcoran, B. A.; Jacobson, M. Z.

2013-12-01

As renewable energy generators are increasingly being installed throughout the U.S., there is growing interest in interconnecting diverse renewable generators (primarily wind and solar) across large geographic areas through an enhanced transmission system. This reduces variability in the aggregate power output, increases system reliability, and allows for the development of the best overall group of renewable technologies and sites to meet the load. Studies are therefore needed to determine the most efficient and economical plan to achieve large area interconnections in a future electric system with a high penetration of renewables. This research quantifies the effects of aggregating electric load together with diverse renewable generation throughout the ten Federal Energy Regulatory Commission (FERC) regions in the contiguous U.S. A deterministic linear program has been built in AMPL (A Mathematical Programming Language) to solve for the least-cost organizational structure and system (generators, transmission, and storage) for a highly renewable electric grid. The analysis will 1) examine a highly renewable 2006 electric system, including various sensitivity cases and additional system components such as additional load from electric vehicles, and 2) create a 'roadmap' from the existing 2006 system to a highly renewable system in 2030, accounting for projected price and demand changes and generator retirements based on age and environmental regulations. Ideally, results from this study will offer insight for a federal renewable energy policy (such as a renewable portfolio standard) and how to best organize U.S. regions for transmission planning.

Renewable energy systems in Mexico: Installation of a hybrid system

Science.gov (United States)

Pate, Ronald C.

1993-05-01

Sandia has been providing technical leadership on behalf of DOE and CORECT on a working level cooperative program with Mexico on renewable energy (PROCER). As part of this effort, the Sandia Design Assistance Center (DAC) and the solar energy program staff at Instituto de Investigaciones Electricas (IIE) in Cuernavaca, Mexico, recently reached agreement on a framework for mutually beneficial technical collaboration on the monitoring and field evaluation of renewable energy systems in Mexico, particularly village-scale hybrid systems. This trip was made for the purpose of planning the details for the joint installation of a data acquisition system (DAS) on a recently completed PV/Wind/Diesel hybrid system in the village of Xcalac on the Southeast coast of the state of Quintana Roo, Mexico. The DAS installation will be made during the week of March 15, 1993. While in Mexico, discussions were also held with personnel from.the National Autonomous University of Mexico (UNAM) Solar Energy Laboratory and several private sector companies with regard to renewable energy project activities and technical and educational support needs in Mexico.

Renewable energy systems: A societal and technological platform

Energy Technology Data Exchange (ETDEWEB)

Polatidis, Heracles; Haralambopoulos, Dias A. [University of the Aegean, Mytilene (Greece). Department of Environment

2007-02-15

Today, the analysis of renewable energy places the emphasis on the technological and economic attributes with social and environmental impact assessment providing for a rather static, narrow frame of analysis. The participation and response of social actors and other stakeholders is usually of a traditional type, with consultation documents and public meetings, collection of complaints and suggestion schemes. This often encourages parochialism and an over-concentration on relatively trivial issues. It is, therefore, imperative to establish a new participatory planning platform to incorporate the wider socio-economic aspects of renewable energy systems and to provide for an operational analytical decomposition of them. In this work the issue of decomposition analysis is clarified, and a new agenda for the societal and technological decomposition analysis of renewable energy systems is developed. A case study is disclosed to present the relevance of the established platform for integrated (renewable) energy systems planning. Innovative aspects comprise of the simultaneous inclusion of decision analysis and social acceptance methods and tools in concert with the related public participation techniques. (author)

EGFR/Src/Akt signaling modulates Sox2 expression and self-renewal of stem-like side-population cells in non-small cell lung cancer.

Science.gov (United States)

Singh, Sandeep; Trevino, Jose; Bora-Singhal, Namrata; Coppola, Domenico; Haura, Eric; Altiok, Soner; Chellappan, Srikumar P

2012-09-25

Cancer stem cells are thought to be responsible for the initiation and progression of cancers. In non-small cell lung cancers (NSCLCs), Hoechst 33342 dye effluxing side population (SP) cells are shown to have stem cell like properties. The oncogenic capacity of cancer stem-like cells is in part due to their ability to self-renew; however the mechanistic correlation between oncogenic pathways and self-renewal of cancer stem-like cells has remained elusive. Here we characterized the SP cells at the molecular level and evaluated its ability to generate tumors at the orthotopic site in the lung microenvironment. Further, we investigated if the self-renewal of SP cells is dependent on EGFR mediated signaling. SP cells were detected and isolated from multiple NSCLC cell lines (H1650, H1975, A549), as well as primary human tumor explants grown in nude mice. SP cells demonstrated stem-like properties including ability to self-renew and grow as spheres; they were able to generate primary and metastatic tumors upon orthotopic implantation into the lung of SCID mice. In vitro study revealed elevated expression of stem cell associated markers like Oct4, Sox2 and Nanog as well as demonstrated intrinsic epithelial to mesenchymal transition features in SP cells. Further, we show that abrogation of EGFR, Src and Akt signaling through pharmacological or genetic inhibitors suppresses the self-renewal growth and expansion of SP-cells and resulted in specific downregulation of Sox2 protein expression. siRNA mediated depletion of Sox2 significantly blocked the SP phenotype as well as its self-renewal capacity; whereas other transcription factors like Oct4 and Nanog played a relatively lesser role in regulating self-renewal. Interestingly, Sox2 was elevated in metastatic foci of human NSCLC samples. Our findings suggest that Sox2 is a novel target of EGFR-Src-Akt signaling in NSCLCs that modulates self-renewal and expansion of stem-like cells from NSCLC. Therefore, the outcome of the

EGFR/Src/Akt signaling modulates Sox2 expression and self-renewal of stem-like side-population cells in non-small cell lung cancer

Directory of Open Access Journals (Sweden)

Singh Sandeep

2012-09-01

Full Text Available Abstract Background Cancer stem cells are thought to be responsible for the initiation and progression of cancers. In non-small cell lung cancers (NSCLCs, Hoechst 33342 dye effluxing side population (SP cells are shown to have stem cell like properties. The oncogenic capacity of cancer stem-like cells is in part due to their ability to self-renew; however the mechanistic correlation between oncogenic pathways and self-renewal of cancer stem-like cells has remained elusive. Here we characterized the SP cells at the molecular level and evaluated its ability to generate tumors at the orthotopic site in the lung microenvironment. Further, we investigated if the self-renewal of SP cells is dependent on EGFR mediated signaling. Results SP cells were detected and isolated from multiple NSCLC cell lines (H1650, H1975, A549, as well as primary human tumor explants grown in nude mice. SP cells demonstrated stem-like properties including ability to self-renew and grow as spheres; they were able to generate primary and metastatic tumors upon orthotopic implantation into the lung of SCID mice. In vitro study revealed elevated expression of stem cell associated markers like Oct4, Sox2 and Nanog as well as demonstrated intrinsic epithelial to mesenchymal transition features in SP cells. Further, we show that abrogation of EGFR, Src and Akt signaling through pharmacological or genetic inhibitors suppresses the self-renewal growth and expansion of SP-cells and resulted in specific downregulation of Sox2 protein expression. siRNA mediated depletion of Sox2 significantly blocked the SP phenotype as well as its self-renewal capacity; whereas other transcription factors like Oct4 and Nanog played a relatively lesser role in regulating self-renewal. Interestingly, Sox2 was elevated in metastatic foci of human NSCLC samples. Conclusions Our findings suggest that Sox2 is a novel target of EGFR-Src-Akt signaling in NSCLCs that modulates self-renewal and expansion of

Career Directions--Renewable Energy Systems Integrator

Science.gov (United States)

Fleeman, Stephen R.

2012-01-01

Renewable energy systems are beginning to appear everywhere. Solar modules are creating "blue roofs" that convert the energy from the sun into household electricity. Solar thermal systems on roofs can generate hot water. Wind turbines catch breezes to provide even more electricity. Recommendations for saving energy, specifying systems for…

Argonaute-2-null embryonic stem cells are retarded in self-renewal ...

Indian Academy of Sciences (India)

Present address: Institute of Stem Cells and Regenerative Medicine, Bangalore, India ... [Chandra Shekar P, Naim A, Partha Sarathi D and Kumar S 2011 Argonaute-2-null embryonic stem cells are retarded in self-renewal ..... Research, India.

ER stress inducer tunicamycin suppresses the self-renewal of glioma-initiating cell partly through inhibiting Sox2 translation.

Science.gov (United States)

Xing, Yang; Ge, Yuqing; Liu, Chanjuan; Zhang, Xiaobiao; Jiang, Jianhai; Wei, Yuanyan

2016-06-14

Glioma-initiating cells possess tumor-initiating potential and are relatively resistant to conventional chemotherapy and irradiation. Therefore, their elimination is an essential factor for the development of efficient therapy. Here, we report that endoplasmic reticulum (ER) stress inducer tunicamycin inhibits glioma-initiating cell self-renewal as determined by neurosphere formation assay. Moreover, tunicamycin decreases the efficiency of glioma-initiating cell to initiate tumor formation. Although tunicamycin induces glioma-initiating cell apoptosis, apoptosis inhibitor z-VAD-fmk only partly abrogates the reduction in glioma-initiating cell self-renewal induced by tunicamycin. Indeed, tunicamycin reduces the expression of self-renewal regulator Sox2 at translation level. Overexpression of Sox2 obviously abrogates the reduction in glioma-initiating cell self-renewal induced by tunicamycin. Taken together, tunicamycin suppresses the self-renewal and tumorigenic potential of glioma-initiating cell partly through reducing Sox2 translation. This finding provides a cue to potential effective treatment of glioblastoma through controlling stem cells.

Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells

Science.gov (United States)

Butler, Jason M.; Nolan, Daniel J.; L.Vertes, Eva; Varnum-Finney, Barbara; Kobayashi, Hideki; Hooper, Andrea T.; Seandel, Marco; Shido, Koji; White, Ian A.; Kobayashi, Mariko; Witte, Larry; May, Chad; Shawber, Carrie; Kimura, Yuki; Kitajewski, Jan; Rosenwaks, Zev; Bernstein, Irwin D.; Rafii, Shahin

2010-01-01

Bone marrow endothelial cells (ECs) are essential for reconstitution of hematopoiesis, but their role in self-renewal of long term-hematopoietic stem cells (LT-HSCs) is unknown. We have developed angiogenic models to demonstrate that EC-derived angiocrine growth factors support in vitro self-renewal and in vivo repopulation of authentic LT-HSCs. In serum/cytokine-free co-cultures, ECs through direct cellular contact, stimulated incremental expansion of repopulating CD34−Flt3−cKit+Lineage−Sca1+ LT-HSCs, which retained their self-renewal ability, as determined by single cell and serial transplantation assays. Angiocrine expression of Notch-ligands by ECs promoted proliferation and prevented exhaustion of LT-HSCs derived from wild-type, but not Notch1/Notch2 deficient mice. In transgenic notch-reporter (TNR.Gfp) mice, regenerating TNR.Gfp+ LT-HSCs were detected in cellular contact with sinusoidal ECs and interfering with angiocrine, but not perfusion function, of SECs impaired repopulation of TNR.Gfp+ LT-HSCs. ECs establish an instructive vascular niche for clinical scale expansion of LT-HSCs and a cellular platform to identify stem cell-active trophogens. PMID:20207228

Achieving a 100% Renewable Grid: Operating Electric Power Systems with Extremely High Levels of Variable Renewable Energy

Energy Technology Data Exchange (ETDEWEB)

Kroposki, Benjamin; Johnson, Brian; Zhang, Yingchen; Gevorgian, Vahan; Denholm, Paul; Hodge, Bri-Mathias; Hannegan, Bryan

2017-03-01

What does it mean to achieve a 100% renewable grid? Several countries already meet or come close to achieving this goal. Iceland, for example, supplies 100% of its electricity needs with either geothermal or hydropower. Other countries that have electric grids with high fractions of renewables based on hydropower include Norway (97%), Costa Rica (93%), Brazil (76%), and Canada (62%). Hydropower plants have been used for decades to create a relatively inexpensive, renewable form of energy, but these systems are limited by natural rainfall and geographic topology. Around the world, most good sites for large hydropower resources have already been developed. So how do other areas achieve 100% renewable grids? Variable renewable energy (VRE), such as wind and solar photovoltaic (PV) systems, will be a major contributor, and with the reduction in costs for these technologies during the last five years, large-scale deployments are happening around the world.

Somatic ACE regulates self-renewal of mouse spermatogonial stem cells via the MAPK signaling pathway.

Science.gov (United States)

Gao, Tingting; Zhao, Xin; Liu, Chenchen; Shao, Binbin; Zhang, Xi; Li, Kai; Cai, Jinyang; Wang, Su; Huang, Xiaoyan

2018-05-24

Spermatogonial stem cell (SSC) self-renewal is an indispensable part of spermatogenesis. Angiotensin I-converting enzyme (ACE) is a zinc dipeptidyl carboxypeptidase that plays a critical role in regulation of the renin-angiotensin system. Here, we used RT-PCR and Western blot analysis to confirm that somatic ACE (sACE) but not testicular ACE (tACE) is highly expressed in mouse testis before postpartum day 7 and in cultured SSCs. Our results revealed that sACE is located on the membrane of SSCs. Treating cultured SSCs with the ACE competitive inhibitor captopril was found to inhibit sACE activity, and significantly reduced the proliferation rate of SSCs. Microarray analysis identified 651 genes with significant differential expression. KEGG pathway analysis showed that these differentially expressed genes are mainly involved in the mitogen-activated protein kinase (MAPK) signaling pathway and cell cycle. sACE was found to play an important role in SSC self-renewal via the regulation of MAPK-dependent cell proliferation.

Metering systems and demand-side management models applied to hybrid renewable energy systems in micro-grid configuration

International Nuclear Information System (INIS)

Blasques, L.C.M.; Pinho, J.T.

2012-01-01

This paper proposes a demand-side management model integrated to a metering system for hybrid renewable energy systems in micro-grid configuration. The proposal is based on the management problems verified in most of this kind of renewable hybrid systems installed in Brazil. The main idea is the implementation of a pre-paid metering system with some control functions that directly act on the consumer demand, restricting the consumption proportionally to the monthly availability of renewable energy. The result is a better distribution of the electricity consumption by month and by consumer, preventing that only one user, with larger purchasing power, consumes all the renewable energy available at some time period. The proportionality between the consumption and the renewable energy's availability has the objective to prevent a lack of energy stored and a high use of the diesel generator-set on months of low renewable potential. This paper also aims to contribute to the Brazilian regulation of renewable energy systems supplying micro-grids. - Highlights: ► Review of the Brazilian electricity regulation for small-scale isolated systems. ► Renewable systems are the most feasible option in several isolated communities. ► One proposal is to guarantee government subsidies for renewable energy systems. ► Smart electronic meters to create electricity restrictions for the consumers.

Activin pathway enhances colorectal cancer stem cell self-renew and tumor progression.

Science.gov (United States)

Liu, Rui; Wang, Jun-Hua; Xu, Chengxiong; Sun, Bo; Kang, Sa-Ouk

2016-10-28

Activin belongs to transforming growth factor (TGF)-β super family of growth and differentiation factors and activin pathway participated in broad range of cell process. Studies elaborated activin pathway maintain pluripotency in human stem cells and suggest that the function of activin/nodal signaling in self-renew would be conserved across embryonic and adult stem cells. In this study, we tried to determine the effect of activin signaling pathway in regulation of cancer stem cells as a potential target for cancer therapy in clinical trials. A population of colorectal cancer cells was selected by the treatment of activin A. This population of cell possessed stem cell character with sphere formation ability. We demonstrated activin pathway enhanced the colorectal cancer stem cells self-renew and contribute to colorectal cancer progression in vivo. Targeting activin pathway potentially provide effective strategy for colorectal cancer therapy. Copyright © 2016 Elsevier Inc. All rights reserved.

The effects of electrospun substrate-mediated cell colony morphology on the self-renewal of human induced pluripotent stem cells.

Science.gov (United States)

Maldonado, Maricela; Wong, Lauren Y; Echeverria, Cristina; Ico, Gerardo; Low, Karen; Fujimoto, Taylor; Johnson, Jed K; Nam, Jin

2015-05-01

The development of xeno-free, chemically defined stem cell culture systems has been a primary focus in the field of regenerative medicine to enhance the clinical application of pluripotent stem cells (PSCs). In this regard, various electrospun substrates with diverse physiochemical properties were synthesized utilizing various polymer precursors and surface treatments. Human induced pluripotent stem cells (IPSCs) cultured on these substrates were characterized by their gene and protein expression to determine the effects of the substrate physiochemical properties on the cells' self-renewal, i.e., proliferation and the maintenance of pluripotency. The results showed that surface chemistry significantly affected cell colony formation via governing the colony edge propagation. More importantly, when surface chemistry of the substrates was uniformly controlled by collagen conjugation, the stiffness of substrate was inversely related to the sphericity, a degree of three dimensionality in colony morphology. The differences in sphericity subsequently affected spontaneous differentiation of IPSCs during a long-term culture, implicating that the colony morphology is a deciding factor in the lineage commitment of PSCs. Overall, we show that the capability of controlling IPSC colony morphology by electrospun substrates provides a means to modulate IPSC self-renewal. Copyright © 2015 Elsevier Ltd. All rights reserved.

A theoretical study of rotatable renewable energy system for stratospheric airship

International Nuclear Information System (INIS)

Lv, Mingyun; Li, Jun; Zhu, Weiyu; Du, Huafei; Meng, Junhui; Sun, Kangwen

2017-01-01

Highlights: • A new rotatable renewable energy system is designed for stratospheric airship. • A theoretical model of optimal rotation angle and required area are studied. • The effects of latitude and date on output energy per day are investigated. • The advantages of the rotatable renewable energy system are studied. - Abstract: Renewable energy system is very critical for solving the energy problem of a long endurance stratospheric airship. Output performance of the traditional solar array fixed on the upper surface of the airship remains to be improved to reduce the area and weight of renewable energy system. Inspired by the solar tracking system and kirigami, a rotatable renewable energy system (mainly including solar array) is designed to improve the current status of the energy system. The advantages of the rotatable solar array are studied using a MATLAB computer program based on the theoretical model established in this paper. The improvements in output energy and required area of the solar array were compared between the traditional airship and improved one. Studies had shown that the rotatable renewable energy system made the total weight of energy system decreased by 1000 kg when the maximum design speed of the airship was greater than 22 m/s. The results demonstrate that the rotatable renewable energy system for the airship can be a good way to improve the output performance of solar array, and the conceptual design and theoretical model suggest a pathway towards solving the energy problem of a stratospheric airship.

Fip1 regulates mRNA alternative polyadenylation to promote stem cell self-renewal

Science.gov (United States)

Lackford, Brad; Yao, Chengguo; Charles, Georgette M; Weng, Lingjie; Zheng, Xiaofeng; Choi, Eun-A; Xie, Xiaohui; Wan, Ji; Xing, Yi; Freudenberg, Johannes M; Yang, Pengyi; Jothi, Raja; Hu, Guang; Shi, Yongsheng

2014-01-01

mRNA alternative polyadenylation (APA) plays a critical role in post-transcriptional gene control and is highly regulated during development and disease. However, the regulatory mechanisms and functional consequences of APA remain poorly understood. Here, we show that an mRNA 3′ processing factor, Fip1, is essential for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Fip1 promotes stem cell maintenance, in part, by activating the ESC-specific APA profiles to ensure the optimal expression of a specific set of genes, including critical self-renewal factors. Fip1 expression and the Fip1-dependent APA program change during ESC differentiation and are restored to an ESC-like state during somatic reprogramming. Mechanistically, we provide evidence that the specificity of Fip1-mediated APA regulation depends on multiple factors, including Fip1-RNA interactions and the distance between APA sites. Together, our data highlight the role for post-transcriptional control in stem cell self-renewal, provide mechanistic insight on APA regulation in development, and establish an important function for APA in cell fate specification. PMID:24596251

Wave power integration with a renewable hydrogen energy system. Paper no. IGEC-1-085

International Nuclear Information System (INIS)

St. Germain, L.; Wild, P.; Rowe, A.

2005-01-01

In British Columbia, approximately 90% of the electricity generated comes from hydroelectric facilities while another abundant and renewable resource, ocean wave energy, is not being utilized at all. Technologies exist that can capture and convert wave energy but there are few studies examining systemic integration of wave energy devices. This work examines the potential to use wave energy as an input into a hydrogen-based renewable energy system. A model of an oscillating water column (OWC) was developed as a module within TRNSYS where it can be coupled to other existing hydrogen-specific components such as an electrolyser, storage device, and fuel cell. The OWC model accounts for device geometry, dynamics, and generator efficiency. For this particular study, wave profiles generated from hourly average data for a location on the west coast of Vancouver Island are used as a resource input. An analysis of the potential to utilise wave energy is carried out with an emphasis on overall system efficiency and resulting device scaling. The results of the integration of wave energy with other renewable energy inputs into a hydrogen-based system are used to make recommendations regarding technical feasibility of wave power projects on Vancouver Island. (author)

Self-renewal and chemotherapy resistance of p75NTR positive cells in esophageal squamous cell carcinomas

International Nuclear Information System (INIS)

Huang, Sheng-Dong; Yuan, Yang; Liu, Xiao-Hong; Gong, De-Jun; Bai, Chen-Guang; Wang, Feng; Luo, Jun-Hui; Xu, Zhi-Yun

2009-01-01

p75 NTR has been used to isolate esophageal and corneal epithelial stem cells. In the present study, we investigated the expression of p75 NTR in esophageal squamous cell carcinoma (ESCC) and explored the biological properties of p75 NTR+ cells. p75 NTR expression in ESCC was assessed by immunohistochemistry. p75 NTR+ and p75 NTR- cells of 4 ESCC cell lines were separated by fluorescence-activated cell sorting. Differentially expressed genes between p75 NTR+ and p75 NTR- cells were determined by real-time quantitative reverse transcription-PCR. Sphere formation assay, DDP sensitivity assay, 64 copper accumulation assay and tumorigenicity analysis were performed to determine the capacity of self-renewal, chemotherapy resistance and tumorigenicity of p75 NTR+ cells. In ESCC specimens, p75 NTR was found mainly confined to immature cells and absent in cells undergoing terminal differentiation. The percentage of p75 NTR+ cells was 1.6%–3.7% in Eca109 and 3 newly established ESCC cell lines. The expression of Bmi-1, which is associated with self-renewal of stem cells, was significantly higher in p75 NTR+ cells. p63, a marker identified in keratinocyte stem cells, was confined mainly to p75 NTR+ cells. The expression of CTR1, which is associated with cisplatin (DDP)-resistance, was significantly decreased in p75 NTR+ cells. Expression levels of differentiation markers, such as involucrin, cytokeratin 13, β1-integrin and β4-integrin, were lower in p75 NTR+ cells. In addition, p75 NTR+ cells generated both p75 NTR+ and p75 NTR- cells, and formed nonadherent spherical clusters in serum-free medium supplemented with growth factors. Furthermore, p75 NTR+ cells were found to be more resistant to DDP and exhibited lower 64 copper accumulation than p75 NTR- cells. Our results demonstrated that p75 NTR+ cells possess some characteristics of CSCs, namely, self-renewal and chemotherapy resistance. Chemotherapy resistance of p75 NTR+ cells may probably be attributable to

Simulation of hybrid renewable microgeneration systems for variable electricity prices

International Nuclear Information System (INIS)

Brandoni, C.; Renzi, M.; Caresana, F.; Polonara, F.

2014-01-01

This paper addresses a hybrid renewable system that consists of a micro-Combined Cooling Heat and Power (CCHP) unit and a solar energy conversion device. In addition to a traditional PV system, a High Concentrator Photovoltaic (HCPV) device, the design of which is suitable for building integration application, was also modelled and embedded in the hybrid system. The work identifies the optimal management strategies for the hybrid renewable system in an effort to minimise the primary energy usage, the carbon dioxide emissions and the operational costs for variable electricity prices that result from the day-ahead electricity market. An “ad hoc” model describes the performance of the HCPV module, PV and Internal Combustion Engine, whilst the other units were simulated based on their main characteristic parameters. The developed algorithm was applied to three different building typologies. The results indicate that the best configuration is the hybrid renewable system with PV, which can provide a yearly primary energy reduction of between 20% and 30% compared to separate production. The hybrid renewable system with HCPV becomes competitive with the PV technology when the level of solar radiation is high. - Highlights: • The paper addresses a hybrid renewable system that consists of a micro-CCHP unit and a solar energy conversion device. • Both PV and High Concentrator Photovoltaic (HCPV) systems have been modelled and embedded in the hybrid system. • The work identifies the optimal management strategies for variable electricity prices. • Hybrid renewable systems provide a yearly primary energy reduction of between 20% and 30% compared to separate production. • When the level of solar radiation is high, HCPV becomes competitive with the PV technology

Cytokinetic Analysis of Slowly Renewing Bone-Marrow Cells after Administration of Nitrogen Mustard

Energy Technology Data Exchange (ETDEWEB)

Haas, R.; Fliedner, T. M.; Stehle, H. [Abteilung fuer Klinische Physiologie der Universitaet Ulm, Ulm/Donau, Federal Republic of Germany (Germany)

1968-08-15

The continuous or repeated administration of tritiated thymidine into pregnant rats during organogenesis provides a method for the complete labelling of newborn rats. If these are continuously injected with tritiated thymidine for the first four weeks after birth, the fraction of labelled cells of all organs and cell-renewal systems is still 100% If completely labelled animals are sacrificed at regular intervals after the discontinuance of thymidine administration, one can distinguish two groups of cells with distinct differences in their cell renewal. While the reticular cells A and B, the endothelial cells and the bone-marrow lymphocytes belong to a slowly proliferating group of cells, the differentiated myelopoietic and erythropoietic cells of the bone marrow proliferate rapidly. That labelled erythropoietic or myelopoietic cells are not found later than 6-10 days after discontinuance of tritated thymidine injection in these animals argues strongly against the hypothesis that under normal steady-state conditions a G{sub 0} fraction exists in the bone-marrow, from which stem cells are deviated into the differentiated cell pools by adequate stimuli. The administration of nitrogen mustard in a dose sufficient to cause bone-marrow aplasia neither destroys nor stimulates the reticular cells and endothelial cells of the bone-marrow matrix. These cells retain their label and remain present in normal numbers throughout the period of observation after nitrogen mustard treatment: The only cell type in the marrow that changes its labelling intensity after nitrogen mustard administration is the marrow lymphocyte. The decrease in the fraction and intensity of labelled bone-marrow lymphocytes precedes the rapid regeneration of nitrogen mustard aplastic bone-marrow. This cell type, in our opinion, would be the only cell to qualify as a stem cell, although positive evidence is still lacking. (author)

Energy System Analysis of 100 Per cent Renewable Energy Systems

DEFF Research Database (Denmark)

Lund, Henrik; Mathiesen, Brian Vad

2007-01-01

This paper presents the methodology and results of the overall energy system analysis of a 100 per cent renewable energy system. The input for the systems is the result of a project of the Danish Association of Engineers, in which 1600 participants during more than 40 seminars discussed...... and designed a model for the future energy system of Denmark, putting emphasis on energy efficiency, CO2 reduction, and industrial development. The energy system analysis methodology includes hour by hour computer simulations leading to the design of flexible energy systems with the ability to balance...... the electricity supply and demand and to exchange electricity productions on the international electricity markets. The results are detailed system designs and energy balances for two energy target years: year 2050 with 100 per cent renewable energy from biomass and combinations of wind, wave and solar power...

SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating cells.

Science.gov (United States)

Santini, R; Pietrobono, S; Pandolfi, S; Montagnani, V; D'Amico, M; Penachioni, J Y; Vinci, M C; Borgognoni, L; Stecca, B

2014-09-18

Melanoma is one of the most aggressive types of human cancer, characterized by enhanced heterogeneity and resistance to conventional therapy at advanced stages. We and others have previously shown that HEDGEHOG-GLI (HH-GLI) signaling is required for melanoma growth and for survival and expansion of melanoma-initiating cells (MICs). Recent reports indicate that HH-GLI signaling regulates a set of genes typically expressed in embryonic stem cells, including SOX2 (sex-determining region Y (SRY)-Box2). Here we address the function of SOX2 in human melanomas and MICs and its interaction with HH-GLI signaling. We find that SOX2 is highly expressed in melanoma stem cells. Knockdown of SOX2 sharply decreases self-renewal in melanoma spheres and in putative melanoma stem cells with high aldehyde dehydrogenase activity (ALDH(high)). Conversely, ectopic expression of SOX2 in melanoma cells enhances their self-renewal in vitro. SOX2 silencing also inhibits cell growth and induces apoptosis in melanoma cells. In addition, depletion of SOX2 progressively abrogates tumor growth and leads to a significant decrease in tumor-initiating capability of ALDH(high) MICs upon xenotransplantation, suggesting that SOX2 is required for tumor initiation and for continuous tumor growth. We show that SOX2 is regulated by HH signaling and that the transcription factors GLI1 and GLI2, the downstream effectors of HH-GLI signaling, bind to the proximal promoter region of SOX2 in primary melanoma cells. In functional studies, we find that SOX2 function is required for HH-induced melanoma cell growth and MIC self-renewal in vitro. Thus SOX2 is a critical factor for self-renewal and tumorigenicity of MICs and an important mediator of HH-GLI signaling in melanoma. These findings could provide the basis for novel therapeutic strategies based on the inhibition of SOX2 for the treatment of a subset of human melanomas.

Stochastic hybrid systems with renewal transitions

NARCIS (Netherlands)

Guerreiro Tome Antunes, D.J.; Hespanha, J.P.; Silvestre, C.J.

2010-01-01

We consider Stochastic Hybrid Systems (SHSs) for which the lengths of times that the system stays in each mode are independent random variables with given distributions. We propose an analysis framework based on a set of Volterra renewal-type equations, which allows us to compute any statistical

Web based system for renewable energy sources assessment

International Nuclear Information System (INIS)

Shtrakov, Stanko; Ivanov, Lachezar South

2011-01-01

The RES A ssess software is developed to assist in the preliminary assessment of potential renewable energy projects. The first release includes water pumping solar systems, passive solar systems, wind, and geothermal energy and biomass. The program guides the users in the design of their systems, by providing initial estimates. By changing the system’s parameters, users are able to quickly screen an effective technology and system size depending on load, climatic conditions, and season of use. This paper describes scope of models (radiation, wind, geothermal, heat transfer) used to predict energy production from energy resource systems, climatic variables and system parameters and software technology for realising the project. Keywords: Energy projects, Renewable energy, Web system

Integrated transport and renewable energy systems

DEFF Research Database (Denmark)

Mathiesen, B.V.; Lund, H.; Nørgård, Per Bromand

2007-01-01

Governments worldwide aim at reducing CO2 emissions and expanding renewable energy. A key element in achieving such a goal is to use renewable energy in transport such as biofuels. However, efforts to promote single transport technologies and single fuels only represent a partial solution...... transport. It is concluded that a 100 per cent renewable energy transport system is possible but is connected to significant challenges in the path towards it. Biomass is a limited resource and it is important to avoid effecting the production. The integration of the transport with the remaining energy....... No single technology can solve the problem of ever increasing CO2 emissions from transport. Transport must be integrated into energy planning, as electricity and heating. In this paper, a coherent effort to integrate transport into energy planning is proposed, using multiple means promoting sustainable...

Design for Reliability in Renewable Energy Systems

DEFF Research Database (Denmark)

Blaabjerg, Frede; Zhou, Dao; Sangwongwanich, Ariya

2017-01-01

Power electronics are widely used in renewable energy systems to achieve lower cost of energy, higher efficiency and high power density. At the same time, the high reliability of the power electronics products is demanded, in order to reduce the failure rates and ensure cost-effective operation...... of the renewable energy systems. This paper thus describes the basic concepts used in reliability engineering, and presents the status and future trends of Design for Reliability (DfR) in power electronics, which is currently undergoing a paradigm shift to a physics-of-failure approach. Two case studies of a 2 MW...

Power Electronics and Reliability in Renewable Energy Systems

DEFF Research Database (Denmark)

Blaabjerg, Frede; Ma, Ke; Zhou, Dao

2012-01-01

Power Electronics are needed in almost all kind of renewable energy systems. It is used both for controlling the renewable source and also for interfacing to the load, which can be grid-connected or working in stand-alone mode. More and more efforts are put into making renewable energy systems...... better in terms of reliability in order to ensure a high availability of the power sources, in this case the knowledge of mission profile of a certain application is crucial for the reliability evaluation/design of power electronics. In this paper an overview on the power electronic circuits behind...... the most common converter configurations for wind turbine and photovoltaic is done. Next different aspects of improving the system reliability are mapped. Further on examples of how to control the chip temperature in different power electronic configurations as well as operation modes for wind power...

Aubergine and piRNAs promote germline stem cell self-renewal by repressing the proto-oncogene Cbl.

Science.gov (United States)

Rojas-Ríos, Patricia; Chartier, Aymeric; Pierson, Stéphanie; Simonelig, Martine

2017-11-02

PIWI proteins play essential roles in germ cells and stem cell lineages. In Drosophila , Piwi is required in somatic niche cells and germline stem cells (GSCs) to support GSC self-renewal and differentiation. Whether and how other PIWI proteins are involved in GSC biology remains unknown. Here, we show that Aubergine (Aub), another PIWI protein, is intrinsically required in GSCs for their self-renewal and differentiation. Aub needs to be loaded with piRNAs to control GSC self-renewal and acts through direct mRNA regulation. We identify the Cbl proto-oncogene, a regulator of mammalian hematopoietic stem cells, as a novel GSC differentiation factor. Aub stimulates GSC self-renewal by repressing Cbl mRNA translation and does so in part through recruitment of the CCR4-NOT complex. This study reveals the role of piRNAs and PIWI proteins in controlling stem cell homeostasis via translational repression and highlights piRNAs as major post-transcriptional regulators in key developmental decisions. © 2017 The Authors. Published under the terms of the CC BY 4.0 license.

Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells

OpenAIRE

Soucie, E.L.; Weng, Z.; Geirsdottir, L.; Molawi, K.; Maurizio, J.; Fenouil, R.; Mossadegh-Keller, N.; Gimenez, G.; VanHille, L.; Beniazza, M.; Favret, J.; Berruyer, C.; Perrin, P.; Hacohen, N.; Andrau, J.C.

2016-01-01

Differentiated macrophages can self-renew in tissues and expand long-term in culture, but the gene regulatory mechanisms that accomplish self-renewal in the differentiated state have remained unknown. Here we show that in mice, the transcription factors MafB and c-Maf repress a macrophage-specific enhancer repertoire associated with a gene network controlling self-renewal. Single cell analysis revealed that, in vivo, proliferating resident macrophages can access this network by transient down...

Cell renewal of glomerular cell types in normal rats. An autoradiographic analysis

International Nuclear Information System (INIS)

Pabst, R.; Sterzel, R.B.

1983-01-01

Normal adult Sprague-Dawley rats received either a single or repetitive injection of the DNA precursor 3 H-thymidine ( 3 H-TdR). For autoradiography semi-thin sections were prepared 2 hr to 14 days after labeling. The majority of labeled cells noted in glomerular tufts were endothelial cells. Mesangial cells had a lower production rate. Podocytes revealed no evidence of proliferation. Bowman's capsule cells showed a higher labeling index than tuft cells at all times. Neither the urinary nor the vascular pole was found to be a proliferative zone for Bowman's capsule cells. The flash and repetitive labeling experiments demonstrated a constant rate of cell renewal of about 1% per day, resulting in a long life span for endothelial and mesangial cells as well as Bowman's capsule cells. These data provide a basis for cell kinetic studies in models of glomerular diseases

Foundations for Protecting Renewable-Rich Distribution Systems.

Energy Technology Data Exchange (ETDEWEB)

Ellis, Abraham [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Brahma, Sukumar [New Mexico State Univ., Las Cruces, NM (United States); Ranade, Satish [New Mexico State Univ., Las Cruces, NM (United States)

2016-11-01

High proliferation of Inverter Interfaced Distributed Energy Resources (IIDERs) into the electric distribution grid introduces new challenges to protection of such systems. This is because the existing protection systems are designed with two assumptions: 1) system is single-sourced, resulting in unidirectional fault current, and (2) fault currents are easily detectable due to much higher magnitudes compared to load currents. Due to the fact that most renewables interface with the grid though inverters, and inverters restrict their current output to levels close to the full load currents, both these assumptions are no longer valid - the system becomes multi-sourced, and overcurrent-based protection does not work. The primary scope of this study is to analyze the response of a grid-tied inverter to different faults in the grid, leading to new guidelines on protecting renewable-rich distribution systems.

A 100% renewable energy system in the year 2050: The case of Macedonia

International Nuclear Information System (INIS)

Ćosić, Boris; Krajačić, Goran; Duić, Neven

2012-01-01

The most important problems the energy sector faces in Macedonia are an unfavourable energy mix with a high prevalence of lignite, a strong dependence on energy import, poor condition of the energy system and inefficiency in energy production and use. This paper investigates the prospects for realization of the 100% renewable energy system in Macedonia by making use of the EnergyPLAN model. Analysis was conducted for two renewable scenarios designed for the years 2030 and 2050. First scenario, the 50% renewable energy system, has been created for the year 2030 and represents the first step towards the 100% renewable energy future of Macedonia. The second scenario has been designed for the 100% renewable energy system based only on the renewable energy sources (RES) in the year 2050. Special attention in the design of these systems has been given to intermittent RES and to storage technologies. The analysis reveals that at the moment the 50% renewable energy system seems much more likely than the 100% renewable energy system, but with additional energy efficiency measures, which will lead to a decrease of consumption and with installation of new generation capacities this goal can be easily achieved.

Methamphetamine decreases dentate gyrus stem cell self-renewal and shifts the differentiation towards neuronal fate

Directory of Open Access Journals (Sweden)

Sofia Baptista

2014-09-01

Full Text Available Methamphetamine (METH is a highly addictive psychostimulant drug of abuse that negatively interferes with neurogenesis. In fact, we have previously shown that METH triggers stem/progenitor cell death and decreases neuronal differentiation in the dentate gyrus (DG. Still, little is known regarding its effect on DG stem cell properties. Herein, we investigate the impact of METH on mice DG stem/progenitor cell self-renewal functions. METH (10 nM decreased DG stem cell self-renewal, while 1 nM delayed cell cycle in the G0/G1-to-S phase transition and increased the number of quiescent cells (G0 phase, which correlated with a decrease in cyclin E, pEGFR and pERK1/2 protein levels. Importantly, both drug concentrations (1 or 10 nM did not induce cell death. In accordance with the impairment of self-renewal capacity, METH (10 nM decreased Sox2+/Sox2+ while increased Sox2−/Sox2− pairs of daughter cells. This effect relied on N-methyl-d-aspartate (NMDA signaling, which was prevented by the NMDA receptor antagonist, MK-801 (10 μM. Moreover, METH (10 nM increased doublecortin (DCX protein levels consistent with neuronal differentiation. In conclusion, METH alters DG stem cell properties by delaying cell cycle and decreasing self-renewal capacities, mechanisms that may contribute to DG neurogenesis impairment followed by cognitive deficits verified in METH consumers.

Hedgehog-GLI signaling drives self-renewal and tumorigenicity of human melanoma-initiating cells.

Science.gov (United States)

Santini, Roberta; Vinci, Maria C; Pandolfi, Silvia; Penachioni, Junia Y; Montagnani, Valentina; Olivito, Biagio; Gattai, Riccardo; Pimpinelli, Nicola; Gerlini, Gianni; Borgognoni, Lorenzo; Stecca, Barbara

2012-09-01

The question of whether cancer stem/tumor-initiating cells (CSC/TIC) exist in human melanomas has arisen in the last few years. Here, we have used nonadherent spheres and the aldehyde dehydrogenase (ALDH) enzymatic activity to enrich for CSC/TIC in a collection of human melanomas obtained from a broad spectrum of sites and stages. We find that melanomaspheres display extensive in vitro self-renewal ability and sustain tumor growth in vivo, generating human melanoma xenografts that recapitulate the phenotypic composition of the parental tumor. Melanomaspheres express high levels of Hedgehog (HH) pathway components and of embryonic pluripotent stem cell factors SOX2, NANOG, OCT4, and KLF4. We show that human melanomas contain a subset of cells expressing high ALDH activity (ALDH(high)), which is endowed with higher self-renewal and tumorigenic abilities than the ALDH(low) population. A good correlation between the number of ALDH(high) cells and sphere formation efficiency was observed. Notably, both pharmacological inhibition of HH signaling by the SMOOTHENED (SMO) antagonist cyclopamine and GLI antagonist GANT61 and stable expression of shRNA targeting either SMO or GLI1 result in a significant decrease in melanoma stem cell self-renewal in vitro and a reduction in the number of ALDH(high) melanoma stem cells. Finally, we show that interference with the HH-GLI pathway through lentiviral-mediated silencing of SMO and GLI1 drastically diminishes tumor initiation of ALDH(high) melanoma stem cells. In conclusion, our data indicate an essential role of the HH-GLI1 signaling in controlling self-renewal and tumor initiation of melanoma CSC/TIC. Targeting HH-GLI1 is thus predicted to reduce the melanoma stem cell compartment. Copyright © 2012 AlphaMed Press.

Impacts of intermittent renewable generation on electricity system costs

International Nuclear Information System (INIS)

Batalla-Bejerano, Joan; Trujillo-Baute, Elisa

2016-01-01

A successful deployment of power generation coming from variable renewable sources, such as wind and solar photovoltaic, strongly depends on the economic cost of system integration. This paper, in seeking to look beyond the impact of renewable generation on the evolution of the total economic costs associated with the operation of the electricity system, aims to estimate the sensitivity of balancing market requirements and costs to the variable and non-fully predictable nature of intermittent renewable generation. The estimations reported in this paper for the Spanish electricity system stress the importance of both attributes as well as power system flexibility when accounting for the cost of balancing services. - Highlights: •A successful deployment of VRES-E strongly depends on the economic cost of its integration. •We estimate the sensitivity of balancing market requirements and costs to VRES-E. •Integration costs depend on variability, predictability and system flexibility.

Optimal Design Of Renewable Energy Systemusing Genetic Algorithm Case Study In Parangtritis

Directory of Open Access Journals (Sweden)

SRI UTAMI

2018-03-01

Tourism has a massive contribution to regional development and shares environmental issues. Reducing reliances on fossil fuel, it is not still adequating energy consumption yet to cause development of renewable energy in crucial position for tourism desicition. An optimal configuration of renewable energy system was planned by Genetic Algorithm in this work. This research conducted to optimize renewable  energy system in Parangtritis, Kretek, Bantul Central Java. The system consisted of solar cells and wind turbines, and the batteries and fuel cells were as storage system. The algorithm minimize objective function of total cost consisted of investment, replacement as well as operating and maintenance costs. A reability evaluation of the system was given by Equivalent Loss Factor (. This index inform an insufficient energy in the systems. The simulation showed an optimum size of the system, achieved by size of PV, battery, wind turbine, electrolizer, hidrogen tank and fuel cell were  3, 48, 36, 3, 2, 8 respectively. The ELF used in this simulation was 0.01. Keywords: fossil fuel,  renewable energy, tourism, Equivalent Lost Factor

Nuclear Energy and Renewables. System Effects in Low-carbon Electricity Systems - Executive Summary

International Nuclear Information System (INIS)

2012-01-01

This report addresses the increasingly important interactions of variable renewables and dispatchable energy technologies, such as nuclear power, in terms of their effects on electricity systems. These effects add costs to the production of electricity, which are not usually transparent. The report recommends that decision-makers should take into account such system costs and internalise them according to a 'generator pays' principle, which is currently not the case. Analysing data from six OECD/NEA countries, the study finds that including the system costs of variable renewables at the level of the electricity grid increases the total costs of electricity supply by up to one-third, depending on technology, country and penetration levels. In addition, it concludes that, unless the current market subsidies for renewables are altered, dispatchable technologies will increasingly not be replaced as they reach their end of life and consequently security of supply will suffer. This implies that significant changes in management and cost allocation will be needed to generate the flexibility required for an economically viable coexistence of nuclear energy and renewables in increasingly de-carbonised electricity systems. (authors)

100% Renewable energy systems, climate mitigation and economic growth

DEFF Research Database (Denmark)

Vad Mathiesen, Brian; Lund, Henrik; Karlsson, Kenneth Bernard

2011-01-01

that implementing energy savings, renewable energy and more efficient conversion technologies can have positive socio-economic effects, create employment and potentially lead to large earnings on exports. If externalities such as health effects are included, even more benefits can be expected. 100% Renewable energy......Greenhouse gas mitigation strategies are generally considered costly with world leaders often engaging in debate concerning the costs of mitigation and the distribution of these costs between different countries. In this paper, the analyses and results of the design of a 100% renewable energy...... system by the year 2050 are presented for a complete energy system including transport. Two short-term transition target years in the process towards this goal are analysed for 2015 and 2030. The energy systems are analysed and designed with hour-by-hour energy system analyses. The analyses reveal...

Puerarin Suppresses the Self-Renewal of Murine Embryonic Stem Cells by Inhibition of REST-MiR-21 Regulatory Pathway.

Science.gov (United States)

Yin, Mengmeng; Yuan, Yin; Cui, Yurong; Hong, Xian; Luo, Hongyan; Hu, Xinwu; Tang, Ming; Hescheler, Jurgen; Xi, Jiaoya

2015-01-01

Puerarin shows a wide range of biological activities, including affecting the cardiac differentiation from murine embryonic stem (mES) cells. However, little is known about its effect and mechanism of action on the self-renewal of mES cells. This study aimed to determine the effect of puerarin on the self-renewal and pluripotency of mES cells and its underlying mechanisms. RT-PCR and real-time PCR were used to detect the transcripts of core transcription factors, specific markers for multiple lineages, REST and microRNA-21 (miR-21). Colony-forming assay was performed to estimate the self-renewal capacity of mES cells. Western blotting and wortmannin were employed to explore the role of PI3K/Akt signaling pathway in the inhibitory action of puerarin on REST transcript. Transfected mES cells with antagomir21 were used to confirm the role of miR-21 in the action of puerarin on cell self-renewal. Puerarin significantly decreased the percentage of the self-renewal colonies, and suppressed the transcripts of Oct4, Nanog, Sox2, c-Myc and REST. Besides, PECAM, NCAM and miR-21 were up-regulated both under the self-renewal conditions and at day 4 of differentiation. The PI3K inhibitor wortmannin successfully reversed the mRNA expression changes of REST, Nanog and Sox2. Transfection of antagomir21 efficiently reversed the effects of puerarin on mES cells self-renewal. Inhibition of REST-miR-21 regulatory pathway may be the key mechanism of puerarin-induced suppression of mES cells self-renewal.

Fuel cells and electrolysers in future energy systems

DEFF Research Database (Denmark)

Mathiesen, Brian Vad

be considered which fuels such technologies can utilise and how these fuels can be distributed. Natural gas is not an option in future renewable energy systems and the de‐ mand for gaseous fuels, such as biogas or syngas, will increase significantly. Hence, fuel cell CHP plants represent a more fuel...... of transport, battery electric vehicles are more suitable than hydrogen fuel cell vehicles in future energy system. Battery electric ve‐ hicles may, for a part of the transport demand, have limitations in their range. Hybrid tech‐ nologies may provide a good option, which can combine the high fuel efficiency......Efficient fuel cells and electrolysers are still at the development stage. In this dissertation, future developed fuel cells and electrolysers are analysed in future renewable energy sys‐ tems. Today, most electricity, heat and transport demands are met by combustion tech‐ nologies. Compared...

Photoactivated Fuel Cells (PhotoFuelCells. An alternative source of renewable energy with environmental benefits

Directory of Open Access Journals (Sweden)

Stavroula Sfaelou

2016-03-01

Full Text Available This work is a short review of Photoactivated Fuel Cells, that is, photoelectrochemical cells which consume an organic or inorganic fuel to produce renewable electricity or hydrogen. The work presents the basic features of photoactivated fuel cells, their modes of operation, the materials, which are frequently used for their construction and some ideas of cell design both for electricity and solar hydrogen production. Water splitting is treated as a special case of photoactivated fuel cell operation.

Seeding of single hemopoietic stem cells and self renewal of committed stem cells

International Nuclear Information System (INIS)

Brecher, G.

1986-01-01

Single cells and two to five proliferating cells were transfused into mice whose own stem cells had been killed by irradiation. When a small inoculum of 50,000 AB marrow cells was given only 4 of 20 recipients survived, but all 4 had only PGK A enzyme in their peripheral blood cells. The results indicate that the survivors received a single pluripotential stem cell capable of proliferating. Survivors showed no deterioration in their blood picture after many months. It was concluded that there is no clonal succession in the marrow cells. Further studies with transfusions of 100,000 and 10,000,000 marrow cells after lethal irradiation suggest that there is production of committed stem cells with significant self-renewal

Risk management of non-renewable energy systems

CERN Document Server

Verma, Ajit Kumar; Muruva, Hari Prasad

2015-01-01

This book describes the basic concepts of risk and reliability with detailed descriptions of the different levels of probabilistic safety assessment of nuclear power plants (both internal and external). The book also maximizes readers insights into time dependent risk analysis through several case studies, whilst risk management with respect to non renewable energy sources is also explained. With several advanced reactors utilizing the concept of passive systems, the reliability estimation of these systems are explained in detail with the book providing a reliability estimation of components through mechanistic model approach. This book is useful for advanced undergraduate and post graduate students in nuclear engineering, aerospace engineering, industrial engineering, reliability and safety engineering, systems engineering and applied probability and statistics. This book is also suitable for one-semester graduate courses on risk management of non renewable energy systems in all conventional engineering bran...

Leptin and Adiponectin Modulate the Self-renewal of Normal Human Breast Epithelial Stem Cells.

Science.gov (United States)

Esper, Raymond M; Dame, Michael; McClintock, Shannon; Holt, Peter R; Dannenberg, Andrew J; Wicha, Max S; Brenner, Dean E

2015-12-01

Multiple mechanisms are likely to account for the link between obesity and increased risk of postmenopausal breast cancer. Two adipokines, leptin and adiponectin, are of particular interest due to their opposing biologic functions and associations with breast cancer risk. In the current study, we investigated the effects of leptin and adiponectin on normal breast epithelial stem cells. Levels of leptin in human adipose explant-derived conditioned media positively correlated with the size of the normal breast stem cell pool. In contrast, an inverse relationship was found for adiponectin. Moreover, a strong linear relationship was observed between the leptin/adiponectin ratio in adipose conditioned media and breast stem cell self-renewal. Consistent with these findings, exogenous leptin stimulated whereas adiponectin suppressed breast stem cell self-renewal. In addition to local in-breast effects, circulating factors, including leptin and adiponectin, may contribute to the link between obesity and breast cancer. Increased levels of leptin and reduced amounts of adiponectin were found in serum from obese compared with age-matched lean postmenopausal women. Interestingly, serum from obese women increased stem cell self-renewal by 30% compared with only 7% for lean control serum. Taken together, these data suggest a plausible explanation for the obesity-driven increase in postmenopausal breast cancer risk. Leptin and adiponectin may function as both endocrine and paracrine/juxtacrine factors to modulate the size of the normal stem cell pool. Interventions that disrupt this axis and thereby normalize breast stem cell self-renewal could reduce the risk of breast cancer. ©2015 American Association for Cancer Research.

On-Site Renewable Energy and Green Buildings: A System-Level Analysis.

Science.gov (United States)

Al-Ghamdi, Sami G; Bilec, Melissa M

2016-05-03

Adopting a green building rating system (GBRSs) that strongly considers use of renewable energy can have important environmental consequences, particularly in developing countries. In this paper, we studied on-site renewable energy and GBRSs at the system level to explore potential benefits and challenges. While we have focused on GBRSs, the findings can offer additional insight for renewable incentives across sectors. An energy model was built for 25 sites to compute the potential solar and wind power production on-site and available within the building footprint and regional climate. A life-cycle approach and cost analysis were then completed to analyze the environmental and economic impacts. Environmental impacts of renewable energy varied dramatically between sites, in some cases, the environmental benefits were limited despite the significant economic burden of those renewable systems on-site and vice versa. Our recommendation for GBRSs, and broader policies and regulations, is to require buildings with higher environmental impacts to achieve higher levels of energy performance and on-site renewable energy utilization, instead of fixed percentages.

Is there dependence and systemic risk between oil and renewable energy stock prices?

International Nuclear Information System (INIS)

Reboredo, Juan C.

2015-01-01

We study systemic risk and dependence between oil and renewable energy markets using copulas to characterize the dependence structure and to compute the conditional value-at-risk as a measure of systemic risk. We found significant time-varying average and symmetric tail dependence between oil returns and several global and sectoral renewable energy indices. Our evidence on systemic risk indicates that oil price dynamics significantly contributes around 30% to downside and upside risk of renewable energy companies. These results have important implications for risk management and renewable energy policies. - Highlights: • We study systemic risk and dependence between oil and renewable energy markets. • Dependence and conditional value-at-risk is obtained through copulas. • Oil and renewable energy displayed time-varying average and symmetric tail dependence. • Oil price contribution to the downside and upside risks of renewable energy companies was around 30%

Management of a power system based on renewable energy

Directory of Open Access Journals (Sweden)

Ronay Karoly

2012-06-01

Full Text Available This article main purpose is to highlight the main advantage of the hardware and software implementation for an energy management system based on renewable energy sources. By using implemented and dedicated hardware and software the evolution of energy production and consumption can be monitored. The advantages of such system are highlighted by the results obtained from experimental simulations. An experimental model for the power system based on renewable energy sources was implemented, where the actual status of the system in different situations when the equipments change their own statuses can be shown.

Cancer Modeling: From Optimal Cell Renewal to Immunotherapy

Science.gov (United States)

Alvarado Alvarado, Cesar Leonardo

Cancer is a disease caused by mutations in normal cells. According to the National Cancer Institute, in 2016, an estimated 1.6 million people were diagnosed and approximately 0.5 million people died from the disease in the United States. There are many factors that shape cancer at the cellular and organismal level, including genetic, immunological, and environmental components. In this thesis, we show how mathematical modeling can be used to provide insight into some of the key mechanisms underlying cancer dynamics. First, we use mathematical modeling to investigate optimal homeostatic cell renewal in tissues such as the small intestine with an emphasis on division patterns and tissue architecture. We find that the division patterns that delay the accumulation of mutations are strictly associated with the population sizes of the tissue. In particular, patterns with long chains of differentiation delay the time to observe a second-hit mutant, which is important given that for many cancers two mutations are enough to initiate a tumor. We also investigated homeostatic cell renewal under a selective pressure and find that hierarchically organized tissues act as suppressors of selection; we find that an architecture with a small number of stem cells and larger pools of transit amplifying cells and mature differentiated cells, together with long chains of differentiation, form a robust evolutionary strategy to delay the time to observe a second-hit mutant when mutations acquire a fitness advantage or disadvantage. We also formulate a model of the immune response to cancer in the presence of costimulatory and inhibitory signals. We demonstrate that the coordination of such signals is crucial to initiate an effective immune response, and while immunotherapy has become a promising cancer treatment over the past decade, these results offer some explanations for why it can fail.

Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells.

Science.gov (United States)

Zhang, Xiaoli; Shi, Kun; Li, Yi; Zhang, Haiyu; Hao, Jing

2014-06-01

Lipopolysaccharide (LPS) can reduce sperm count and sperm quality. The molecular mechanisms underlying this process are not fully understood. In this report, we investigated the effects of LPS-treated Sertoli cells on self-renewal and differentiation of spermatogoinial stem cells (SSCs). Sertoli cell cultures were established and incubated with LPS (10μg/ml) for 1, 2 or 3 days, respectively. The culture media were collected and used as conditioned media (CM) to culture SSCs. The expression of glial cell-derived neurotrophic factor (GDNF), stem cell factor (SCF) and bone morphogenetic protein 4 (BMP4) in Sertoli cells treated with LPS was analyzed by RT-PCR and Western blotting. The results showed that the expression of SSC differentiation markers, c-kit and Sohlh2, was increased, while the expression of SSC self-renewal markers, plzf, oct4, and PCNA, was repressed when cultured in CM from LPS-treated Sertoli cells. GDNF levels in Sertoli cells and CM reduced dramatically after LPS treatments, while SCF and BMP4 levels did not show any significant changes. Moreover, correlated with the GDNF levels in CM, GDNF target genes, Bcl6b and Etv5, were reduced markedly in SSCs. Our results suggest that LPS inhibits the expression of GDNF in Sertoli cells, and might prevent the SSC self-renewal via down-regulation of GDNF target genes. Copyright © 2014 Elsevier Inc. All rights reserved.

Effects of the Endocrine-Disrupting Chemical DDT on Self-Renewal and Differentiation of Human Mesenchymal Stem Cells

Science.gov (United States)

Strong, Amy L.; Shi, Zhenzhen; Strong, Michael J.; Miller, David F.B.; Rusch, Douglas B.; Buechlein, Aaron M.; Flemington, Erik K.; McLachlan, John A.; Nephew, Kenneth P.

2014-01-01

Background: Although the global use of the endocrine-disrupting chemical DDT has decreased, its persistence in the environment has resulted in continued human exposure. Accumulating evidence suggests that DDT exposure has long-term adverse effects on development, yet the impact on growth and differentiation of adult stem cells remains unclear. Objectives: Human mesenchymal stem cells (MSCs) exposed to DDT were used to evaluate the impact on stem cell biology. Methods: We assessed DDT-treated MSCs for self-renewal, proliferation, and differentiation potential. Whole genome RNA sequencing was performed to assess gene expression in DDT-treated MSCs. Results: MSCs exposed to DDT formed fewer colonies, suggesting a reduction in self-renewal potential. DDT enhanced both adipogenic and osteogenic differentiation, which was confirmed by increased mRNA expression of glucose transporter type 4 (GLUT4), lipoprotein lipase (LpL), peroxisome proliferator-activated receptor gamma (PPARγ), leptin, osteonectin, core binding factor 1 (CBFA1), and FBJ murine osteosarcoma viral oncogene homolog (c-Fos). Expression of factors in DDT-treated cells was similar to that in estrogen-treated MSCs, suggesting that DDT may function via the estrogen receptor (ER)-mediated pathway. The coadministration of ICI 182,780 blocked the effects of DDT. RNA sequencing revealed 121 genes and noncoding RNAs to be differentially expressed in DDT-treated MSCs compared with controls cells. Conclusion: Human MSCs provide a powerful biological system to investigate and identify the molecular mechanisms underlying the effects of environmental agents on stem cells and human health. MSCs exposed to DDT demonstrated profound alterations in self-renewal, proliferation, differentiation, and gene expression, which may partially explain the homeostatic imbalance and increased cancer incidence among those exposed to long-term EDCs. Citation: Strong AL, Shi Z, Strong MJ, Miller DF, Rusch DB, Buechlein AM, Flemington EK

The first step towards a 100% renewable energy-system for Ireland

DEFF Research Database (Denmark)

Connolly, D.; Lund, Henrik; Mathiesen, Brian Vad

2011-01-01

, it is essential that Ireland begins to utilise its renewable resources more effectively. Therefore, this study presents the first step towards a 100% renewable energy-system for Ireland. The energy-system analysis tool used was EnergyPLAN, as it accounts for all sectors of the energy-system that need...

Feasibility of hydrogen from renewable energy in the Arctic

International Nuclear Information System (INIS)

Chauhan, B.

2004-01-01

'Full text:' There is an abundance of renewable resources in the Canadian Arctic. Despite that diesel is still the conventional source used by homes and businesses for their electrical and space heating needs. Electrolysis of water to produce hydrogen using renewable resources is under investigation. A techno-economic feasibility has been conducted for hybrid systems including wind turbine, photovoltaic system, electrolyser and fuel cells. Different scenarios have been considered for meeting the needs of a small, remote community in the Arctic. Results will be presented indicating the most cost-effective Wind-PV-Electrolyser-Fuel Cell system for combined heat and power. (author)

Hybrid PV/Wind Power Systems Incorporating Battery Storage and Considering the Stochastic Nature of Renewable Resources

Science.gov (United States)

Barnawi, Abdulwasa Bakr

Hybrid power generation system and distributed generation technology are attracting more investments due to the growing demand for energy nowadays and the increasing awareness regarding emissions and their environmental impacts such as global warming and pollution. The price fluctuation of crude oil is an additional reason for the leading oil producing countries to consider renewable resources as an alternative. Saudi Arabia as the top oil exporter country in the word announced the "Saudi Arabia Vision 2030" which is targeting to generate 9.5 GW of electricity from renewable resources. Two of the most promising renewable technologies are wind turbines (WT) and photovoltaic cells (PV). The integration or hybridization of photovoltaics and wind turbines with battery storage leads to higher adequacy and redundancy for both autonomous and grid connected systems. This study presents a method for optimal generation unit planning by installing a proper number of solar cells, wind turbines, and batteries in such a way that the net present value (NPV) is minimized while the overall system redundancy and adequacy is maximized. A new renewable fraction technique (RFT) is used to perform the generation unit planning. RFT was tested and validated with particle swarm optimization and HOMER Pro under the same conditions and environment. Renewable resources and load randomness and uncertainties are considered. Both autonomous and grid-connected system designs were adopted in the optimal generation units planning process. An uncertainty factor was designed and incorporated in both autonomous and grid connected system designs. In the autonomous hybrid system design model, the strategy including an additional amount of operation reserve as a percent of the hourly load was considered to deal with resource uncertainty since the battery storage system is the only backup. While in the grid-connected hybrid system design model, demand response was incorporated to overcome the impact of

Overview of U.S. programs for hydrogen from renewables

International Nuclear Information System (INIS)

Lewis, M.

2007-01-01

This paper discusses US program for hydrogen from renewable energy sources. Renewable energy sources include biomass, wind, solar, hydropower, geothermal and ocean waves. Although nuclear power is not considered renewable, a case can be made that it is, but requires recycling of spent fuel. The paper also discusses hydrogen production, storage and delivery. It discusses fuel cells, safety codes and standards and system analysis

Battery energy storage systems: Assessment for small-scale renewable energy integration

Energy Technology Data Exchange (ETDEWEB)

Nair, Nirmal-Kumar C.; Garimella, Niraj [Power Systems Group, Department of Electrical and Computer Engineering, The University of Auckland, 38 Princes Street, Science Centre, Auckland 1142 (New Zealand)

2010-11-15

Concerns arising due to the variability and intermittency of renewable energy sources while integrating with the power grid can be mitigated to an extent by incorporating a storage element within the renewable energy harnessing system. Thus, battery energy storage systems (BESS) are likely to have a significant impact in the small-scale integration of renewable energy sources into commercial building and residential dwelling. These storage technologies not only enable improvements in consumption levels from renewable energy sources but also provide a range of technical and monetary benefits. This paper provides a modelling framework to be able to quantify the associated benefits of renewable resource integration followed by an overview of various small-scale energy storage technologies. A simple, practical and comprehensive assessment of battery energy storage technologies for small-scale renewable applications based on their technical merit and economic feasibility is presented. Software such as Simulink and HOMER provides the platforms for technical and economic assessments of the battery technologies respectively. (author)

The effects of silver nanoparticles on mouse embryonic stem cell self-renewal and proliferation

Directory of Open Access Journals (Sweden)

Pavan Rajanahalli

2015-01-01

Full Text Available Silver nanoparticles (AgNPs are gaining rapid popularity in many commonly used medical and commercial products for their unique anti-bacterial properties. The molecular mechanisms of effects of AgNPs on stem cell self-renewal and proliferation have not yet been well understood. The aim of the work is to use mouse embryonic stem cells (mESCs as a cellular model to evaluate the toxicity of AgNPs. mESC is a very special cell type which has self-renewal and differentiation properties. The objective of this project is to determine the effects of AgNPs with different surface chemical compositions on the self-renewal and cell cycle of mESCs. Two different surface chemical compositions of AgNPs, polysaccharide-coated and hydrocarbon-coated, were used to test their toxic effects on self-renewal and proliferation of mESCs. The results indicated that both polysaccharide-coated and hydrocarbon-coated AgNPs changed the cell morphology of mESCs. Cell cycle analysis indicated that AgNPs induced mESCs cell cycle arrest at G1 and S phases through inhibition of the hyperphosphorylation of Retinoblastoma (Rb protein. Furthermore, AgNPs exposure reduced Oct4A isoform expression which is responsible for the pluripotency of mESCs, and induced the expression of several isoforms OCT4B-265, OCT4B-190, OCT4B-164 which were suggested involved in stem cell stresses responses. In addition, the evidence of reactive oxygen species (ROS production with two different surface chemical compositions of AgNPs supported our hypothesis that the toxic effect AgNPs exposure is due to overproduction of ROS which altered the gene expression and protein modifications. Polysaccharide coating reduced ROS production, and thus reduced the AgNPs toxicity.

Integrating renewable energy sources in the Portuguese power system

International Nuclear Information System (INIS)

Martins, Nuno; Cabral, Pedro; Azevedo, Helena

2012-01-01

The integration of large amounts of renewable energy is an important challenge for the future management of electric systems, since it affects the operation of the electric power system and the design of the transmission and distribution network infrastructure. This is specially due to the connection requirements of the renewable energy technologies, to the extension and adjustment of the grid infrastructure and to the identification of new solutions for operational reserve, in order to maintain the overall system flexibility and security. In this paper, the impact of high penetration of intermittent energy sources, expected in long term in the Portuguese Power System, is analysed and the operational reserve requirements to accomplish a reliable and reasonable electrical energy supply are identified. It was concluded that pumped storage power plants, special power plants with regulating capabilities, will have an important task to provide the operational reserve requirements of the Portuguese Power System. This technology assumes a fundamental role not only to ensure the adequate levels of security of supply but also to allow the maximum exploitation of the installed capacity in renewable energy sources. (authors)

The histone demethylase Jarid1b is required for hematopoietic stem cell self-renewal

DEFF Research Database (Denmark)

Stewart, Morag H; Albert, Mareike; Sroczynska, Patrycja

2015-01-01

Jarid1b/KDM5b is a histone demethylase that regulates self-renewal and differentiation in stem cells and cancer, however its function in hematopoiesis is unclear. Here, we find that Jarid1b is highly expressed in primitive hematopoietic compartments and is overexpressed in acute myeloid leukemias...... compromises hematopoietic stem cell (HSC) self-renewal capacity and suggest that Jarid1b is a positive regulator of HSC potential.......Jarid1b/KDM5b is a histone demethylase that regulates self-renewal and differentiation in stem cells and cancer, however its function in hematopoiesis is unclear. Here, we find that Jarid1b is highly expressed in primitive hematopoietic compartments and is overexpressed in acute myeloid leukemias....... Constitutive genetic deletion of Jarid1b did not impact steady-state hematopoiesis. In contrast, acute deletion of Jarid1b from bone marrow increased peripheral blood T cells and, following secondary transplantation, resulted in loss of bone marrow reconstitution. Our results reveal that deletion of Jarid1b...

The role of PV electricity generation in fully renewable energy supply systems

International Nuclear Information System (INIS)

Lehmann, H.; Peter, S.

2004-01-01

A sustainable energy supply will be based on renewable energies and it must use available resources efficiently. Earlier or later the energy supply will rely completely on renewable sources. A solar energy system that provides a reliable energy supply throughout the year includes the consistent use of local renewable energy sources (e.g. PV) wherever possible. Using Japan as a example it was shown that the vision of a full renewable energy supply, even with high shares of domestic sources is possible. Detailed simulations of such a system show that the PV systems play an important role delivering electricity at peak demand times. (authors)

A CREB-MPP7-AMOT Regulatory Axis Controls Muscle Stem Cell Expansion and Self-Renewal Competence

Directory of Open Access Journals (Sweden)

Lydia Li

2017-10-01

Full Text Available Summary: Skeletal muscle regeneration requires resident muscle stem cells, termed satellite cells (SCs. SCs are largely quiescent during homeostasis yet become activated upon injury to supply myonuclei and self-renewed SCs. Molecular mechanisms underlying the competence of SCs to proliferate and self-renew in response to injury remain unclear. Here, we show that CREB activity establishes proliferative potential during SC quiescence. SCs with inhibited CREB activity remain quiescent and positioned in their niche, but upon injury, they cannot enter or maintain a proliferative state for expansion and self-renewal. We demonstrate mechanistically that Mpp7 is a CREB target and its functional mediator. MPP7 loss affects the level and sub-cellular localization of AMOT and YAP1 in quiescent SCs. Furthermore, MPP7 and AMOT are required for YAP1 nuclear accumulation, and the three are individually required for a proliferative state in myoblasts. We propose that the CREB-MPP7-AMOT-YAP1 axis establishes the competence of quiescent SCs to expand and self-renew, thereby preserving stem cell function. : Satellite cells are quiescent muscle stem cells that have the ability to regenerate muscles after injury. Li and Fan reveal an MPP7-AMOT-YAP1 regulatory axis that acts downstream of CREB to instill satellite cell competence. They also show how this regulatory axis prepares satellite cells for robust muscle regeneration after injury.

Renewal of cooling system of JMTR

International Nuclear Information System (INIS)

Onoue, Ryuji; Kawamata, Takanori; Otsuka, Kaoru; Koike, Sumio; Nishiyama, Yutaka; Fukasaku, Akitomi

2011-06-01

The Japan Materials Testing Reactor (JMTR) is a light water moderated and cooled tank-type reactor, and its thermal power is 50 MW. The JMTR is categorized as high flux testing reactors in the world. The JMTR has been utilized for irradiation experiments of nuclear fuels and materials, as well as for radioisotope productions since the first criticality in March 1968 until August 2006. JAEA decided to refurbish the JMTR as an important fundamental infrastructure to promote the nuclear research and development. The refurbishment work was started from 2007, and restart is planned in 2011. Renewal facilities were selected from evaluation on their damage and wear in terms of aging. Facilities whose replacement parts are no longer manufactured or not likely to be manufactured continuously in near future, are selected as renewal ones. Replacement priority was decided with special attention to safety concerns. A monitoring of aging condition by the regular maintenance activity is an important factor in selection of continuous using after the restart. In this report, renewal of the cooling system within refurbishment facilities in the JMTR is summarized. (author)

Electrical drives for direct drive renewable energy systems

CERN Document Server

Mueller, Markus

2013-01-01

Wind turbine gearboxes present major reliability issues, leading to great interest in the current development of gearless direct-drive wind energy systems. Offering high reliability, high efficiency and low maintenance, developments in these direct-drive systems point the way to the next generation of wind power, and Electrical drives for direct drive renewable energy systems is an authoritative guide to their design, development and operation. Part one outlines electrical drive technology, beginning with an overview of electrical generators for direct drive systems. Principles of electrical design for permanent magnet generators are discussed, followed by electrical, thermal and structural generator design and systems integration. A review of power electronic converter technology and power electronic converter systems for direct drive renewable energy applications is then conducted. Part two then focuses on wind and marine applications, beginning with a commercial overview of wind turbine drive systems and a...

The integration of renewable energies into the electricity systems of North Africa

International Nuclear Information System (INIS)

Brand, Bernhard

2015-01-01

How can renewable energy sources be efficiently integrated into the North African electricity systems? By using techno-economic modeling methods, this book explores optimized electricity system expansion pathways until the year 2030 for the five North African countries - Morocco, Algeria, Tunisia, Libya and Egypt. The results indicate that renewable energy integration is actually a viable business case for the entire region, if wind and solar capacities are properly planned in conjunction with the conventional generation system and under consideration of the country-specific electricity supply-/demand patterns. Further aspects featured in this publication are the impact of renewable power on the transnational electricity transmission system and the question how decision making processes about renewable energy strategies can be improved in the North African context. The book is a contribution to the scientific literature about energy issues in the Middle East and North Africa (MENA), but also seeks to address political and industrial practitioners concerned with the development of the region's renewable energy future.

The integration of renewable energies into the electricity systems of North Africa

Energy Technology Data Exchange (ETDEWEB)

Brand, Bernhard

2015-11-01

How can renewable energy sources be efficiently integrated into the North African electricity systems? By using techno-economic modeling methods, this book explores optimized electricity system expansion pathways until the year 2030 for the five North African countries - Morocco, Algeria, Tunisia, Libya and Egypt. The results indicate that renewable energy integration is actually a viable business case for the entire region, if wind and solar capacities are properly planned in conjunction with the conventional generation system and under consideration of the country-specific electricity supply-/demand patterns. Further aspects featured in this publication are the impact of renewable power on the transnational electricity transmission system and the question how decision making processes about renewable energy strategies can be improved in the North African context. The book is a contribution to the scientific literature about energy issues in the Middle East and North Africa (MENA), but also seeks to address political and industrial practitioners concerned with the development of the region's renewable energy future.

Establishing long-term cultures with self-renewing acute myeloid leukemia stem/progenitor cells

NARCIS (Netherlands)

van Gosliga, Djoke; Schepers, Hein; Rizo, Aleksandra; van der Kolk, Dorina; Vellenga, Edo; Schuringa, Jan Jacob

2007-01-01

Objective. With the emergence of the concept of the leukemia stem cell, assays to study them remain pivotal in understanding (leukemic) stem cell biology. Methods. We have cultured acute myeloid leukemia CD34(+) cells on bone marrow stroma. Long-term expansion was monitored and self-renewal was

Autonomous Renewable Energy Systems | Van Voorden | Nigerian ...

African Journals Online (AJOL)

The problems of having many renewable sources such as wind and solar generating units in a power system are uncontrollable fluctuations in power generation and the difficulty in forecasting the power generation capability of these sources due to their stochastic nature. Therefore, autonomous electricity systems with a ...

Orphan nuclear receptor TLX activates Wnt/β-catenin signalling to stimulate neural stem cell proliferation and self-renewal

Science.gov (United States)

Qu, Qiuhao; Sun, Guoqiang; Li, Wenwu; Yang, Su; Ye, Peng; Zhao, Chunnian; Yu, Ruth T.; Gage, Fred H.; Evans, Ronald M.; Shi, Yanhong

2010-01-01

The nuclear receptor TLX (also known as NR2E1) is essential for adult neural stem cell self-renewal; however, the molecular mechanisms involved remain elusive. Here we show that TLX activates the canonical Wnt/β-catenin pathway in adult mouse neural stem cells. Furthermore, we demonstrate that Wnt/β-catenin signalling is important in the proliferation and self-renewal of adult neural stem cells in the presence of epidermal growth factor and fibroblast growth factor. Wnt7a and active β-catenin promote neural stem cell self-renewal, whereas the deletion of Wnt7a or the lentiviral transduction of axin, a β-catenin inhibitor, led to decreased cell proliferation in adult neurogenic areas. Lentiviral transduction of active β-catenin led to increased numbers of type B neural stem cells in the subventricular zone of adult brains, whereas deletion of Wnt7a or TLX resulted in decreased numbers of neural stem cells retaining bromodeoxyuridine label in the adult brain. Both Wnt7a and active β-catenin significantly rescued a TLX (also known as Nr2e1) short interfering RNA-induced deficiency in neural stem cell proliferation. Lentiviral transduction of an active β-catenin increased cell proliferation in neurogenic areas of TLX-null adult brains markedly. These results strongly support the hypothesis that TLX acts through the Wnt/β-catenin pathway to regulate neural stem cell proliferation and self-renewal. Moreover, this study suggests that neural stem cells can promote their own self-renewal by secreting signalling molecules that act in an autocrine/paracrine mode. PMID:20010817

Orphan nuclear receptor TLX activates Wnt/beta-catenin signalling to stimulate neural stem cell proliferation and self-renewal.

Science.gov (United States)

Qu, Qiuhao; Sun, Guoqiang; Li, Wenwu; Yang, Su; Ye, Peng; Zhao, Chunnian; Yu, Ruth T; Gage, Fred H; Evans, Ronald M; Shi, Yanhong

2010-01-01

The nuclear receptor TLX (also known as NR2E1) is essential for adult neural stem cell self-renewal; however, the molecular mechanisms involved remain elusive. Here we show that TLX activates the canonical Wnt/beta-catenin pathway in adult mouse neural stem cells. Furthermore, we demonstrate that Wnt/beta-catenin signalling is important in the proliferation and self-renewal of adult neural stem cells in the presence of epidermal growth factor and fibroblast growth factor. Wnt7a and active beta-catenin promote neural stem cell self-renewal, whereas the deletion of Wnt7a or the lentiviral transduction of axin, a beta-catenin inhibitor, led to decreased cell proliferation in adult neurogenic areas. Lentiviral transduction of active beta-catenin led to increased numbers of type B neural stem cells in the subventricular zone of adult brains, whereas deletion of Wnt7a or TLX resulted in decreased numbers of neural stem cells retaining bromodeoxyuridine label in the adult brain. Both Wnt7a and active beta-catenin significantly rescued a TLX (also known as Nr2e1) short interfering RNA-induced deficiency in neural stem cell proliferation. Lentiviral transduction of an active beta-catenin increased cell proliferation in neurogenic areas of TLX-null adult brains markedly. These results strongly support the hypothesis that TLX acts through the Wnt/beta-catenin pathway to regulate neural stem cell proliferation and self-renewal. Moreover, this study suggests that neural stem cells can promote their own self-renewal by secreting signalling molecules that act in an autocrine/paracrine mode.

Chaordic systems for holonic organizational renewal

NARCIS (Netherlands)

Eijnatten, van F.M.; Pasmore, W.A.; Woodman, R.W.

2001-01-01

Socio-Technical Systems Design has a long-standing reputation as an integral approach to organization design. One of its local brands is known as the Dutch approach to Integral Organizational Renewal of the firm (IOR). Central in IOR is an attempt to integrate both business process design,

The cell cycle inhibitor p27Kip¹ controls self-renewal and pluripotency of human embryonic stem cells by regulating the cell cycle, Brachyury and Twist.

Science.gov (United States)

Menchón, Cristina; Edel, Michael J; Izpisua Belmonte, Juan Carlos

2011-05-01

The continued turn over of human embryonic stem cells (hESC) while maintaining an undifferentiated state is dependent on the regulation of the cell cycle. Here we asked the question if a single cell cycle gene could regulate the self-renewal or pluripotency properties of hESC. We identified that the protein expression of the p27(Kip)¹ cell cycle inhibitor is low in hESC cells and increased with differentiation. By adopting a gain and loss of function strategy we forced or reduced its expression in undifferentiating conditions to define its functional role in self-renewal and pluripotency. Using undifferentiation conditions, overexpression of p27(Kip)¹ in hESC lead to a G₁phase arrest with an enlarged and flattened hESC morphology and consequent loss of self-renewal ability. Loss of p27(Kip)¹ caused an elongated/scatter cell-like phenotype involving up-regulation of Brachyury and Twist gene expression. We demonstrate the novel finding that p27(Kip)¹ protein occupies the Twist1 gene promoter and manipulation of p27(Kip)¹ by gain and loss of function is associated with Twist gene expression changes. These results define p27(Kip)¹ expression levels as critical for self-renewal and pluripotency in hESC and suggest a role for p27(Kip)¹ in controlling an epithelial to mesenchymal transition (EMT) in hESC.

A hybrid renewable energy system for a North American off-grid community

International Nuclear Information System (INIS)

Rahman, Md. Mustafizur; Khan, Md. Mohib-Ul-Haque; Ullah, Mohammad Ahsan; Zhang, Xiaolei; Kumar, Amit

2016-01-01

Canada has many isolated communities that are not connected to the electrical grid. Most of these communities meet their electricity demand through stand-alone diesel generators. Diesel generators have economic and environmental concerns that can be minimized by using hybrid renewable energy technologies. This study aims to assess the implementation of a hybrid energy system for an off-grid community in Canada and to propose the best hybrid energy combination to reliably satisfy electricity demand. Seven scenarios were developed: 1) 100% renewable resources, 2) 80% renewable resources, 3) 65% renewable resources, 4) 50% renewable resources, 5) 35% renewable resources, 6) 21% renewable resources, and 7) battery-diesel generators (0% renewable resources). A case study for the remote community of Sandy Lake, Ontario, was conducted. Hybrid systems were chosen to meet the requirements of a 4.4 MWh/day primary load with a 772 kW peak load. Sensitivity analyses were carried out to assess the impact of solar radiation, wind speed, diesel price, CO 2 penalty cost, and project interest rate on optimum results. A GHG (greenhouse gas) abatement cost was assessed for each scenario. Considering GHG emission penalty cost, the costs of electricity for the seven scenarios are $1.48/kWh, $0.62/kWh, $0.54/kWh, $0.42/kWh, $0.39/kWh, $0.37/kWh, and $0.36/kWh. - Highlights: • Modeling of hybrid renewable energy systems for an off-grid community. • Seven scenarios were developed based on various renewable energy fractions. • Cost of electricity is the highest for 100% renewable fraction scenario. • CO 2 emissions are reduced by 1232 tonnes/yr by switching from diesel to renewables. • The electricity cost is most sensitive to diesel price based on sensitivity analysis.

Immortal DNA strand cosegregation requires p53/IMPDH-dependent asymmetric self-renewal associated with adult stem cells.

Science.gov (United States)

Rambhatla, Lakshmi; Ram-Mohan, Sumati; Cheng, Jennifer J; Sherley, James L

2005-04-15

Because they are long-lived and cycle continuously, adult stem cells (ASCs) are predicted as the most common precursor for cancers in adult mammalian tissues. Two unique attributes have been proposed to restrict the carcinogenic potential of ASCs. These are asymmetric self-renewal that limits their number and immortal DNA strand cosegregation that limits their accumulation of mutations due to DNA replication errors. Until recently, the molecular basis and regulation of these important ASC-specific functions were unknown. We developed engineered cultured cells that exhibit asymmetric self-renewal and immortal DNA strand cosegregation. These model cells were used to show that both ASC-specific functions are regulated by the p53 cancer gene. Previously, we proposed that IMP dehydrogenase (IMPDH) was an essential factor for p53-dependent asymmetric self-renewal. We now confirm this proposal and provide quantitative evidence that asymmetric self-renewal is acutely sensitive to even modest changes in IMPDH expression. These analyses reveal that immortal DNA strand cosegregation is also regulated by IMPDH and confirm the original implicit precept that immortal DNA strand cosegregation is specific to cells undergoing asymmetric self-renewal (i.e., ASCs). With IMPDH being the rate-determining enzyme for guanine ribonucleotide (rGNP) biosynthesis, its requirement implicates rGNPs as important regulators of ASC asymmetric self-renewal and immortal DNA strand cosegregation. An in silico analysis of global gene expression data from human cancer cell lines underscored the importance of p53-IMPDH-rGNP regulation for normal tissue cell kinetics, providing further support for the concept that ASCs are key targets for adult tissue carcinogenesis.

Intermittent Stem Cell Cycling Balances Self-Renewal and Senescence of the C. elegans Germ Line.

Directory of Open Access Journals (Sweden)

Amanda Cinquin

2016-04-01

Full Text Available Self-renewing organs often experience a decline in function in the course of aging. It is unclear whether chronological age or external factors control this decline, or whether it is driven by stem cell self-renewal-for example, because cycling cells exhaust their replicative capacity and become senescent. Here we assay the relationship between stem cell cycling and senescence in the Caenorhabditis elegans reproductive system, defining this senescence as the progressive decline in "reproductive capacity," i.e. in the number of progeny that can be produced until cessation of reproduction. We show that stem cell cycling diminishes remaining reproductive capacity, at least in part through the DNA damage response. Paradoxically, gonads kept under conditions that preclude reproduction keep cycling and producing cells that undergo apoptosis or are laid as unfertilized gametes, thus squandering reproductive capacity. We show that continued activity is in fact beneficial inasmuch as gonads that are active when reproduction is initiated have more sustained early progeny production. Intriguingly, continued cycling is intermittent-gonads switch between active and dormant states-and in all likelihood stochastic. Other organs face tradeoffs whereby stem cell cycling has the beneficial effect of providing freshly-differentiated cells and the detrimental effect of increasing the likelihood of cancer or senescence; stochastic stem cell cycling may allow for a subset of cells to preserve proliferative potential in old age, which may implement a strategy to deal with uncertainty as to the total amount of proliferation to be undergone over an organism's lifespan.

Inhibition of CXCL12/CXCR4 autocrine/paracrine loop reduces viability of human glioblastoma stem-like cells affecting self-renewal activity

International Nuclear Information System (INIS)

Gatti, Monica; Pattarozzi, Alessandra; Bajetto, Adriana; Würth, Roberto; Daga, Antonio; Fiaschi, Pietro; Zona, Gianluigi; Florio, Tullio; Barbieri, Federica

2013-01-01

Cancer stem cells (CSCs) or tumor initiating cells (TICs) drive glioblastoma (GBM) development, invasiveness and drug resistance. Distinct molecular pathways might regulate CSC biology as compared to cells in the bulk tumor mass, representing potential therapeutic targets. Chemokine CXCL12 and its receptor CXCR4 control proliferation, invasion and angiogenesis in GBM cell lines and primary cultures, but little is known about their activity in GBM CSCs. We demonstrate that CSCs, isolated from five human GBMs, express CXCR4 and release CXCL12 in vitro, although different levels of expression and secretion were observed in individual cultures, as expected for the heterogeneity of GBMs. CXCL12 treatment induced Akt-mediated significant pro-survival and self-renewal activities, while proliferation was induced at low extent. The role of CXCR4 signaling in CSC survival and self-renewal was further demonstrated using the CXCR4 antagonist AMD3100 that reduced self-renewal and survival with greater efficacy in the cultures that released higher CXCL12 amounts. The specificity of CXCL12 in sustaining CSC survival was demonstrated by the lack of AMD3100-dependent inhibition of viability in differentiated cells derived from the same GBMs. These findings, although performed on a limited number of tumor samples, suggest that the CXCL12/CXCR4 interaction mediates survival and self-renewal in GBM CSCs with high selectivity, thus emerging as a candidate system responsible for maintenance of cancer progenitors, and providing survival benefits to the tumor

Renewables within the German Electricity System - Experiences and Needs

Directory of Open Access Journals (Sweden)

Martin Kaltschmitt

2017-06-01

Full Text Available During the last two decades renewable sources of energy as an environmentally friendly alternative to fossil fuel energy have gained more and more importance within the German electricity system. Their share has increased from less than 4 % to roughly one third of the gross electricity production in the last 25 years. Against this background, the goal of this paper is to present briefly the current status of the use of renewables within the German electricity system, to assess selected developments taking place during this development process as well as to identify given challenges and needs as well as necessary actions to pave the road for a further use of renewable sources of energy within the German electricity provision system. The political driver for the latter is the overarching goal to reduce Greenhouse Gas (GHG emissions which has been confirmed within the Paris agreement signed by the end of 2015.

The Integration of Sustainable Transport into Future Renewable Energy Systems in China

DEFF Research Database (Denmark)

Liu, Wen

use are largely lost in the current fossil fuel dominated energy systems. Sustainable transport development requires solutions from an overall renewable energy system in which integration of large-scale intermittent renewable energy needs assistance. Technologies of alternative vehicle fuels...... in transport may play a role in furthering such integration. The objective of this research is to make a contribution to the development of methodologies to identify and develop future sustainable transport systems as well as to apply such methodologies to the case of China. In particular, the methodological...... development focuses on 1) identifying suitable transport technologies and strategies based on renewable energy and 2) evaluating such technologies from the perspective of overall renewable energy system integration. For this purpose, a methodological framework involving the research fields of both...

Renewable Energy Systems in the Power Electronics Curriculum

DEFF Research Database (Denmark)

Blaabjerg, Frede; Chen, Zhe; Teodorescu, Remus

2005-01-01

of the most important area is renewable energy systems. This paper will discuss the basic courses for the power electronics curriculum. It will also discuss how to teach power electronic systems efficiently through a projectoriented and problem-based learning approach with Aalborg University in Denmark...... as a full-scale example. Different project examples will be given as well as important laboratories for adjustable speed drives and renewable energy systems which are used at the university are described.......Power Electronics is still an emerging technology and its applications are increasing. The primary function is to convert electrical energy from one stage to another and it is used in many different applications. The power electronics curriculum is multidisciplinary covering fields like devices...

An examination of isolated, stationary, hydrogen power systems supplied by renewables: component and system issues and criteria necessary for successful worldwide deployment

Energy Technology Data Exchange (ETDEWEB)

Rambach, G. D. [Energy and Environmental Engineering Center, Desert Research Institute, Reno, NV (United States)

1999-12-01

The premise of this paper is that remote, stationary power systems, based on indigenous renewable energy sources, are an ideal market entry opportunity for hydrogen, but that the deployment of isolated power systems relying on hydrogen as the energy storage medium requires complex and comprehensive planning and design considerations to provide for successful market entry strategies and appropriate systems engineering. Accordingly, this paper sets out to discuss the criteria and the framework necessary to determine how to successfully deploy any specific system or to plan a global marketing strategy. Details of the indigenous intermittent energy sources (wind turbines, solar photovoltaic, micro-hydroelectric, etc), primary power-to-hydrogen conversion systems, hydrogen storage methods, and hydrogen-to-electricity conversion systems (hydrogen-internal combustion engine generator set, hydrogen fuel cells) are described, along with the criteria for technically and commercially successful deployment of any renewable utility power system that employs energy storage.2 refs., 4 figs.

Distinct Stromal Cell Factor Combinations Can Separately Control Hematopoietic Stem Cell Survival, Proliferation, and Self-Renewal

Directory of Open Access Journals (Sweden)

Stefan Wohrer

2014-06-01

Full Text Available Hematopoietic stem cells (HSCs are identified by their ability to sustain prolonged blood cell production in vivo, although recent evidence suggests that durable self-renewal (DSR is shared by HSC subtypes with distinct self-perpetuating differentiation programs. Net expansions of DSR-HSCs occur in vivo, but molecularly defined conditions that support similar responses in vitro are lacking. We hypothesized that this might require a combination of factors that differentially promote HSC viability, proliferation, and self-renewal. We now demonstrate that HSC survival and maintenance of DSR potential are variably supported by different Steel factor (SF-containing cocktails with similar HSC-mitogenic activities. In addition, stromal cells produce other factors, including nerve growth factor and collagen 1, that can antagonize the apoptosis of initially quiescent adult HSCs and, in combination with SF and interleukin-11, produce >15-fold net expansions of DSR-HSCs ex vivo within 7 days. These findings point to the molecular basis of HSC control and expansion.

Analysis of a hybrid renewable energy system on the Mures valley using Homer

Directory of Open Access Journals (Sweden)

Dumitru Cristian Dragoş

2011-12-01

Full Text Available Renewable energy technologies offer the promise of clean, abundant energy gathered from self-renewing resources such as the sun, wind, earth, and plants. Virtually all regions of the world have renewable resources of one type or another. This paper deals with the modeling and analysis of a hybrid system based on renewable energy resources, located on the Mureş valley, using a dedicated software named HOMER. Different types and topologies of renewable resources for the energy supply are analyzed; a small consumer situated on the Mureş Valley is modeled based on a load curve. Finally, the energy flows between the renewable energy system and the local supplying network are analyzed.

Mid-term report on Renewable Energy Forecasting System

International Nuclear Information System (INIS)

Brand, A.J.; Hegberg, T.; Van der Borg, N.J.C.M.; Kok, J.K.; Van Selow, E.R.; Kamphuis, I.G.; De Noord, M.; Van Sambeek, E.J.W.

2001-04-01

The most important conclusions on the economical and technical feasibility of renewable energy forecasting systems are presented, next to recommendations to be followed in order to introduce such a system in the Dutch electricity market. 11 refs

Electric vehicles and renewable energy in the transport sector - energy system consequences. Main focus: Battery electric vehicles and hydrogen based fuel cell vehicles

DEFF Research Database (Denmark)

Nielsen, L.H.; Jørgensen K.

2000-01-01

The aim of the project is to analyse energy, environmental and economic aspects of integrating electric vehicles in the future Danish energy system. Consequences of large-scale utilisation of electric vehicles are analysed. The aim is furthermore toillustrate the potential synergistic interplay...... between the utilisation of electric vehicles and large-scale utilisation of fluctuating renewable energy resources, such as wind power. Economic aspects for electric vehicles interacting with a liberalisedelectricity market are analysed. The project focuses on battery electric vehicles and fuel cell...... vehicles based on hydrogen. Based on assumptions on the future technical development for battery electric vehicles, fuel cell vehicles on hydrogen, and forthe conventional internal combustion engine vehicles, scenarios are set up to reflect expected options for the long-term development of road transport...

Discovery of Power-Law Growth in the Self-Renewal of Heterogeneous Glioma Stem Cell Populations.

Directory of Open Access Journals (Sweden)

Michiya Sugimori

Full Text Available Accumulating evidence indicates that cancer stem cells (CSCs drive tumorigenesis. This suggests that CSCs should make ideal therapeutic targets. However, because CSC populations in tumors appear heterogeneous, it remains unclear how CSCs might be effectively targeted. To investigate the mechanisms by which CSC populations maintain heterogeneity during self-renewal, we established a glioma sphere (GS forming model, to generate a population in which glioma stem cells (GSCs become enriched. We hypothesized, based on the clonal evolution concept, that with each passage in culture, heterogeneous clonal sublines of GSs are generated that progressively show increased proliferative ability.To test this hypothesis, we determined whether, with each passage, glioma neurosphere culture generated from four different glioma cell lines become progressively proliferative (i.e., enriched in large spheres. Rather than monitoring self-renewal, we measured heterogeneity based on neurosphere clone sizes (#cells/clone. Log-log plots of distributions of clone sizes yielded a good fit (r>0.90 to a straight line (log(% total clones = k*log(#cells/clone indicating that the system follows a power-law (y = xk with a specific degree exponent (k = -1.42. Repeated passaging of the total GS population showed that the same power-law was maintained over six passages (CV = -1.01 to -1.17. Surprisingly, passage of either isolated small or large subclones generated fully heterogeneous populations that retained the original power-law-dependent heterogeneity. The anti-GSC agent Temozolomide, which is well known as a standard therapy for glioblastoma multiforme (GBM, suppressed the self-renewal of clones, but it never disrupted the power-law behavior of a GS population.Although the data above did not support the stated hypothesis, they did strongly suggest a novel mechanism that underlies CSC heterogeneity. They indicate that power-law growth governs the self-renewal of heterogeneous

Embryonic stem cell self-renewal pathways converge on the transcription factor Tfcp2l1

Science.gov (United States)

Ye, Shoudong; Li, Ping; Tong, Chang; Ying, Qi-Long

2013-01-01

Mouse embryonic stem cell (mESC) self-renewal can be maintained by activation of the leukaemia inhibitory factor (LIF)/signal transducer and activator of transcription 3 (Stat3) signalling pathway or dual inhibition (2i) of glycogen synthase kinase 3 (Gsk3) and mitogen-activated protein kinase kinase (MEK). Several downstream targets of the pathways involved have been identified that when individually overexpressed can partially support self-renewal. However, none of these targets is shared among the involved pathways. Here, we show that the CP2 family transcription factor Tfcp2l1 is a common target in LIF/Stat3- and 2i-mediated self-renewal, and forced expression of Tfcp2l1 can recapitulate the self-renewal-promoting effect of LIF or either of the 2i components. In addition, Tfcp2l1 can reprogram post-implantation epiblast stem cells to naïve pluripotent ESCs. Tfcp2l1 upregulates Nanog expression and promotes self-renewal in a Nanog-dependent manner. We conclude that Tfcp2l1 is at the intersection of LIF- and 2i-mediated self-renewal pathways and plays a critical role in maintaining ESC identity. Our study provides an expanded understanding of the current model of ground-state pluripotency. PMID:23942238

Energy system analysis of 100% renewable energy systems-The case of Denmark in years 2030 and 2050

DEFF Research Database (Denmark)

Lund, Henrik; Mathiesen, Brian Vad

2009-01-01

for two energy target years: year 2050 with 100% renewable energy from biomass and combinations of wind, wave and solar power; and year 2030 with 50% renewable energy, emphasising the first important steps on the way. The conclusion is that a 100% renewable energy supply based on domestic resources......This paper presents the methodology and results of the overall energy system analysis of a 100% renewable energy system. The input for the systems is the result of a project of the Danish Association of Engineers, in which 1600 participants during more than 40 seminars discussed and designed...... a model for the future energy system of Denmark. The energy system analysis methodology includes hour by hour computer simulations leading to the design of flexible energy systems with the ability to balance the electricity supply and demand. The results are detailed system designs and energy balances...

Large Scale Integration of Renewable Power Sources into the Vietnamese Power System

Science.gov (United States)

Kies, Alexander; Schyska, Bruno; Thanh Viet, Dinh; von Bremen, Lueder; Heinemann, Detlev; Schramm, Stefan

2017-04-01

The Vietnamese Power system is expected to expand considerably in upcoming decades. Power capacities installed are projected to grow from 39 GW in 2015 to 129.5 GW by 2030. Installed wind power capacities are expected to grow to 6 GW (0.8 GW 2015) and solar power capacities to 12 GW (0.85 GW 2015). This goes hand in hand with an increase of the renewable penetration in the power mix from 1.3% from wind and photovoltaics (PV) in 2015 to 5.4% by 2030. The overall potential for wind power in Vietnam is estimated to be around 24 GW. Moreover, the up-scaling of renewable energy sources was formulated as one of the priorized targets of the Vietnamese government in the National Power Development Plan VII. In this work, we investigate the transition of the Vietnamese power system towards high shares of renewables. For this purpose, we jointly optimise the expansion of renewable generation facilities for wind and PV, and the transmission grid within renewable build-up pathways until 2030 and beyond. To simulate the Vietnamese power system and its generation from renewable sources, we use highly spatially and temporally resolved historical weather and load data and the open source modelling toolbox Python for Power System Analysis (PyPSA). We show that the highest potential of renewable generation for wind and PV is observed in southern Vietnam and discuss the resulting need for transmission grid extensions in dependency of the optimal pathway. Furthermore, we show that the smoothing effect of wind power has several considerable beneficial effects and that the Vietnamese hydro power potential can be efficiently used to provide balancing opportunities. This work is part of the R&D Project "Analysis of the Large Scale Integration of Renewable Power into the Future Vietnamese Power System" (GIZ, 2016-2018).

Nuclear-Renewable Hybrid System Economic Basis for Electricity, Fuel, and Hydrogen

Energy Technology Data Exchange (ETDEWEB)

Charles Forsberg; Steven Aumeier

2014-04-01

Concerns about climate change and altering the ocean chemistry are likely to limit the use of fossil fuels. That implies a transition to a low-carbon nuclear-renewable electricity grid. Historically variable electricity demand was met using fossil plants with low capital costs, high operating costs, and substantial greenhouse gas emissions. However, the most easily scalable very-low-emissions generating options, nuclear and non-dispatchable renewables (solar and wind), are capital-intensive technologies with low operating costs that should operate at full capacities to minimize costs. No combination of fully-utilized nuclear and renewables can meet the variable electricity demand. This implies large quantities of expensive excess generating capacity much of the time. In a free market this results in near-zero electricity prices at times of high nuclear renewables output and low electricity demand with electricity revenue collapse. Capital deployment efficiency—the economic benefit derived from energy systems capital investment at a societal level—strongly favors high utilization of these capital-intensive systems, especially if low-carbon nuclear renewables are to replace fossil fuels. Hybrid energy systems are one option for better utilization of these systems that consumes excess energy at times of low prices to make some useful product.The economic basis for development of hybrid energy systems is described for a low-carbon nuclear renewable world where much of the time there are massivequantities of excess energy available from the electric sector.Examples include (1) high-temperature electrolysis to generate hydrogen for non-fossil liquid fuels, direct use as a transport fuel, metal reduction, etc. and (2) biorefineries.Nuclear energy with its concentrated constant heat output may become the enabling technology for economically-viable low-carbon electricity grids because hybrid nuclear systems may provide an economic way to produce dispatachable variable

Control of germline stem cell self-renewal and differentiation in the Drosophila ovary: concerted actions of niche signals and intrinsic factors.

Science.gov (United States)

Xie, Ting

2013-01-01

In the Drosophila ovary, germline stem cells (GSCs) physically interact with their niche composed of terminal filament cells, cap cells, and possibly GSC-contacting escort cells (ECs). A GSC divides to generate a self-renewing stem cell that remains in the niche and a differentiating daughter that moves away from the niche. The GSC niche provides a bone morphogenetic protein (BMP) signal that maintains GSC self-renewal by preventing stem cell differentiation via repression of the differentiation-promoting gene bag of marbles (bam). In addition, it expresses E-cadherin, which mediates cell adhesion for anchoring GSCs in the niche, enabling continuous self-renewal. GSCs themselves also express different classes of intrinsic factors, including signal transducers, transcription factors, chromatin remodeling factors, translation regulators, and miRNAs, which control self-renewal by strengthening interactions with the niche and repressing various differentiation pathways. Differentiated GSC daughters, known as cystoblasts (CBs), also express distinct classes of intrinsic factors to inhibit self-renewal and promote germ cell differentiation. Surprisingly, GSC progeny are also dependent on their surrounding ECs for proper differentiation at least partly by preventing BMP from diffusing to the differentiated germ cell zone and by repressing ectopic BMP expression. Therefore, both GSC self-renewal and CB differentiation are controlled by collaborative actions of extrinsic signals and intrinsic factors. Copyright © 2012 Wiley Periodicals, Inc.

Cross-border versus cross-sector interconnectivity in renewable energy systems

International Nuclear Information System (INIS)

Thellufsen, Jakob Zinck; Lund, Henrik

2017-01-01

In the transition to renewable energy systems, fluctuating renewable energy, such as wind and solar power, plays a large and important role. This creates a challenge in terms of meeting demands, as the energy production fluctuates based on weather patterns. To utilise high amounts of fluctuating renewable energy, the energy system has to be more flexible in terms of decoupling demand and production. This paper investigates two potential ways to increase flexibility. The first is the interconnection between energy systems, for instance between two countries, labelled as cross-border interconnection, and the second is cross-sector interconnection, i.e., the integration between different parts of an energy system, for instance heat and electricity. This paper seeks to compare the types of interconnectivity and discuss to which extent they are mutually beneficial. To do this, the study investigates two energy systems that represent Northern and Southern Europe. Both systems go through three developmental steps that increase the cross-sector interconnectivity. At each developmental step an increasing level of transmission capacities is examined to identify the benefits of cross-border interconnectivity. The results show that while both measures increase the system utilisation of renewable energy and the system efficiency, the cross-sector interconnection gives the best system performance. To analyse the possible interaction between cross-sector and cross-border interconnectivity, two main aspects have to be clarified. The first part defines the approach and the second is the construction of the two archetypes. - Highlights: • A method to investigate system integration and system interconnection is suggested. • The implementation is investigated across a Northern and Southern energy system. • The study identifies benefits of system integration and system interconnection. • The performance of the energy system benefits most from system integration.

Nuclear-Renewable Hybrid Energy Systems: 2016 Technology Development Program Plan

International Nuclear Information System (INIS)

Bragg-Sitton, Shannon M.; Boardman, Richard; Rabiti, Cristian; Suk Kim, Jong; McKellar, Michael; Sabharwall, Piyush; Chen, Jun; Cetiner, M. Sacit; Harrison, T. Jay; Qualls, A. Lou

2016-01-01

The United States is in the midst of an energy revolution, spurred by advancement of technology to produce unprecedented supplies of oil and natural gas. Simultaneously, there is an increasing concern for climate change attributed to greenhouse gas (GHG) emissions that, in large part, result from burning fossil fuels. An international consensus has concluded that the U.S. and other developed nations have an imperative to reduce GHG emissions to address these climate change concerns. The global desire to reduce GHG emissions has led to the development and deployment of clean energy resources and technologies, particularly renewable energy technologies, at a rapid rate. At the same time, each of the major energy sectors-the electric grid, industrial manufacturing, transportation, and the residential/commercial consumers- is increasingly becoming linked through information and communications technologies, advanced modeling and simulation, and controls. Coordination of clean energy generation technologies through integrated hybrid energy systems, as defined below, has the potential to further revolutionize energy services at the system level by coordinating the exchange of energy currency among the energy sectors in a manner that optimizes financial efficiency (including capital investments), maximizes thermodynamic efficiency (through best use of exergy, which is the potential to use the available energy in producing energy services), reduces environmental impacts when clean energy inputs are maximized, and provides resources for grid management. Rapid buildout of renewable technologies has been largely driven by local, state, and federal policies, such as renewable portfolio standards and production tax credits that incentivize investment in these generation sources. A foundational assumption within this program plan is that renewable technologies will continue to be major contributors to the future U.S. energy infrastructure. While increased use of clean renewable

Nuclear-Renewable Hybrid Energy Systems: 2016 Technology Development Program Plan

Energy Technology Data Exchange (ETDEWEB)

Bragg-Sitton, Shannon M. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Boardman, Richard [Idaho National Lab. (INL), Idaho Falls, ID (United States); Rabiti, Cristian [Idaho National Lab. (INL), Idaho Falls, ID (United States); Suk Kim, Jong [Idaho National Lab. (INL), Idaho Falls, ID (United States); McKellar, Michael [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sabharwall, Piyush [Idaho National Lab. (INL), Idaho Falls, ID (United States); Chen, Jun [Idaho National Lab. (INL), Idaho Falls, ID (United States); Cetiner, M. Sacit [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Harrison, T. Jay [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Qualls, A. Lou [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

2016-03-01

The United States is in the midst of an energy revolution, spurred by advancement of technology to produce unprecedented supplies of oil and natural gas. Simultaneously, there is an increasing concern for climate change attributed to greenhouse gas (GHG) emissions that, in large part, result from burning fossil fuels. An international consensus has concluded that the U.S. and other developed nations have an imperative to reduce GHG emissions to address these climate change concerns. The global desire to reduce GHG emissions has led to the development and deployment of clean energy resources and technologies, particularly renewable energy technologies, at a rapid rate. At the same time, each of the major energy sectors—the electric grid, industrial manufacturing, transportation, and the residential/commercial consumers— is increasingly becoming linked through information and communications technologies, advanced modeling and simulation, and controls. Coordination of clean energy generation technologies through integrated hybrid energy systems, as defined below, has the potential to further revolutionize energy services at the system level by coordinating the exchange of energy currency among the energy sectors in a manner that optimizes financial efficiency (including capital investments), maximizes thermodynamic efficiency (through best use of exergy, which is the potential to use the available energy in producing energy services), reduces environmental impacts when clean energy inputs are maximized, and provides resources for grid management. Rapid buildout of renewable technologies has been largely driven by local, state, and federal policies, such as renewable portfolio standards and production tax credits that incentivize investment in these generation sources. A foundational assumption within this program plan is that renewable technologies will continue to be major contributors to the future U.S. energy infrastructure. While increased use of clean

Quantitation and renewal of alveolar and bronchiolar cell populations of rat lungs. Changes during some pathological processes

International Nuclear Information System (INIS)

Fritsch, Paul.

1979-02-01

The various cells of alveolar and bronchiolar tissues of rat lungs were studied qualitatively and quantitatively. In physiological conditions, the renewal rate of the cell populations is low and the frequency of the various cell types is constant. This stability, especially at the level of the alveolar tissue, was also found during the latency period and the development of radiation-induced lung cancers. A particular cellular population was demonstrated: marginated leukocyte pool at the level of the pulmonary circulation. This pool was different both qualitatively and quantitatively from the leukocytes of the systemic circulation and, in physiological conditions, behaved as a cellular reservoir of monocytes chiefly re-distributed according to the body needs. In pathological conditions, its fast migration contributed to the defence of the alveolar medium. A quantitative study of the renewal of alveolar macrophages showed that under 1 p. cent of the marginated leukocyte pool is used daily to keep up this population. This fraction undergoes a maturation stage by cellular division within the endoalveolar medium. In some pathological conditions, this division can be completely inhibited [fr

The Drosophila BCL6 homolog Ken and Barbie promotes somatic stem cell self-renewal in the testis niche.

Science.gov (United States)

Issigonis, Melanie; Matunis, Erika

2012-08-15

Stem cells sustain tissue regeneration by their remarkable ability to replenish the stem cell pool and to generate differentiating progeny. Signals from local microenvironments, or niches, control stem cell behavior. In the Drosophila testis, a group of somatic support cells called the hub creates a stem cell niche by locally activating the Janus Kinase-Signal Transducer and Activator of Transcription (JAK-STAT) pathway in two adjacent types of stem cells: germline stem cells (GSCs) and somatic cyst stem cells (CySCs). Here, we find that ken and barbie (ken) is autonomously required for the self-renewal of CySCs but not GSCs. Furthermore, Ken misexpression in the CySC lineage induces the cell-autonomous self-renewal of somatic cells as well as the nonautonomous self-renewal of germ cells outside the niche. Thus, Ken, like Stat92E and its targets ZFH1 (Leatherman and Dinardo, 2008) and Chinmo (Flaherty et al., 2010), is necessary and sufficient for CySC renewal. However, ken is not a JAK-STAT target in the testis, but instead acts in parallel to Stat92E to ensure CySC self-renewal. Ken represses a subset of Stat92E targets in the embryo (Arbouzova et al., 2006) suggesting that Ken maintains CySCs by repressing differentiation factors. In support of this hypothesis, we find that the global JAK-STAT inhibitor Protein tyrosine phosphatase 61F (Ptp61F) is a JAK-STAT target in the testis that is repressed by Ken. Together, our work demonstrates that Ken has an important role in the inhibition of CySC differentiation. Studies of ken may inform our understanding of its vertebrate orthologue B-Cell Lymphoma 6 (BCL6) and how misregulation of this oncogene leads to human lymphomas. Copyright © 2012 Elsevier Inc. All rights reserved.

Fine-tuning Hematopoiesis: Microenvironmental factors regulating self-renewal and differentiation of hematopoietic stem cells

NARCIS (Netherlands)

T.C. Luis (Tiago)

2010-01-01

markdownabstract__Abstract__ Hematopoietic stem cells (HSCs) have the ability to self renew and generate all lineages of blood cells. Although it is currently well established that hematopoietic stem cells (HSCs) regenerate the blood compartment, it was only in the 1960s that was introduced the

Metabolism and the Control of Cell Fate Decisions and Stem Cell Renewal

Science.gov (United States)

Ito, Kyoko; Ito, Keisuke

2016-01-01

Although the stem cells of various tissues remain in the quiescent state to maintain their undifferentiated state, they also undergo cell divisions as required, and if necessary, even a single stem cell is able to provide for lifelong tissue homeostasis. Stem cell populations are precisely controlled by the balance between their symmetric and asymmetric divisions, with their division patterns determined by whether the daughter cells involved retain their self-renewal capacities. Recent studies have reported that metabolic pathways and the distribution of mitochondria are regulators of the division balance of stem cells and that metabolic defects can shift division balance toward symmetric commitment, which leads to stem cell exhaustion. It has also been observed that in asymmetric division, old mitochondria, which are central metabolic organelles, are segregated to the daughter cell fated to cell differentiation, whereas in symmetric division, young and old mitochondria are equally distributed between both daughter cells. Thus, metabolism and mitochondrial biology play important roles in stem cell fate decisions. As these decisions directly affect tissue homeostasis, understanding their regulatory mechanisms in the context of cellular metabolism is critical. PMID:27482603

Autonomous renewable energy conversion system

Energy Technology Data Exchange (ETDEWEB)

Valtchev, V. [Technical University of Varna (Bulgaria). Dept. of Electronics; Bossche, A. van den; Ghijselen, J.; Melkebeek, J. [University of Gent (Belgium). Dept. of Electrical Power Engineering

2000-02-01

This paper briefly reviews the need for renewable power generation and describes a medium-power Autonomous Renewable Energy Conversion System (ARECS), integrating conversion of wind and solar energy sources. The objectives of the paper are to extract maximum power from the proposed wind energy conversion scheme and to transfer this power and the power derived by the photovoltaic system in a high efficiency way to a local isolated load. The wind energy conversion operates at variable shaft speed yielding an improved annual energy production over constant speed systems. An induction generator (IG) has been used because of its reduced cost, robustness, absence of separate DC source for excitation, easier dismounting and maintenance. The maximum energy transfer of the wind energy is assured by a simple and reliable control strategy adjusting the stator frequency of the IG so that the power drawn is equal to the peak power production of the wind turbine at any wind speed. The presented control strategy also provides an optimal efficiency operation of the IG by applying a quadratic dependence between the IG terminal voltage and frequency V {approx} f{sup 2}. For improving the total system efficiency, high efficiency converters have been designed and implemented. The modular principle of the proposed DC/DC conversion provides the possibility for modifying the system structure depending on different conditions. The configuration of the presented ARECS and the implementation of the proposed control algorithm for optimal power transfer are fully discussed. The stability and dynamic performance as well as the different operation modes of the proposed control and the operation of the converters are illustrated and verified on an experimental prototype. (author)

The Concept of Autonomous Power Supply System Fed with Renewable Energy Sources

Directory of Open Access Journals (Sweden)

Waldemar Fedak

2017-12-01

Full Text Available Sustainable economic development requires the use of renewable energy sources in a rational and thoughtful way. In Polish conditions the use of several types of renewable energy sources on a single setup is a new issue. In particular, hybrid devices in conjunction with intelligent energy systems, such as lighting systems are generally not used. Therefore, the Polish energy production still relies on the burning of coal. Despite their advantages, renewable energy sources are characterized by seasonality and considerable instability. Access to renewable energy varies daily and seasonally, hence activities promoting the use of autonomous, hybrid power systems must be intensified. The presented research aims at the development of the Autonomous Power Supply (APS system based on the so-called energy mix. Such a system works in an isolated arrangement and serves to reliably supply electricity from renewable sources for small residential or public utility devices in an urban area. Systems with up to 3 kW power consist of modules, whose modular design allows the combination of various power configurations and types of renewable energy used. The basic system consists of a primary power source, additional power source, emergency power source, energy storage device, weather station and controller. The energy mix depends on the geographical location of the system. The emergency source can be implemented as an on-grid connector or fuel power generator with the participation of 100% until the primary or accessory power source failure is removed. The energy storage system consists of batteries or supercapacitors. The proposed system can be combined to create a local network that automatically responds to energy shortages in various network nodes by adjusting the supply of electricity within the network depending on its needs. For Poland realistic solutions in this article are the new and modern answer to these requirements.

Technological Decision to Renewable Energy Usage Biogas for Off-grid Systems Consumption

Directory of Open Access Journals (Sweden)

Zubkova Marina

2016-01-01

Full Text Available This paper presents the results of the energy experiments based on electrochemical researches and the thermodynamic calculations, which are carried out on the hydrogenous fuel with the residual content of methane obtained from biogas selected organic waste. Energy indicators are examined in comparison to electrolysis hydrogen. The use of technical and electro physical indicators together with parameters of the fuel operating allowed assessing energy efficiency the module reformer - fuel cell running on a non-standard hydrogenous fuel. Numerical characteristic the efficiency of workflows fuel system reformer – fuel cell is about 39%. To operate the power installation with a predetermined capacity amount used of hydrogenous fuel is comparable to required electrolysis hydrogen amount. Shown the possibility of creation the systems of power supply based on new hydrogen technologies using renewable energy resources local waste. Confirms the relatively high efficiency the usage of hydrogenous fuel for the tasks of off-grid systems consumption.

Power conversion and control methods for renewable energy sources

Science.gov (United States)

Yu, Dachuan

2005-07-01

In recent years, there has been an increase in the use of renewable energy due to the growing concern over the pollution caused by fossil-fuel-based energy. Renewable energy sources, such as photovoltaic (PV) and fuel cell, can be used to enhance the safety, reliability, sustainability, and transmission efficiency of a power system. This dissertation focuses on the power conversion and control for two major renewable-energy sources: PV and fuel cell. Firstly, a current-based, maximum power-point tracking (MPPT) algorithm is proposed for PV energy. An economical converter system using the above scheme for converting the output from PV panels into 60 Hz AC voltage is developed and built. Secondly, a novel circuit model for the Proton Exchange Membrane (PEM) fuel-cell stack that is useful in the design and analysis of fuel-cell-based power systems is proposed. This Pspice-based model uses elements available in the Pspice library with some modifications to represent both the static and dynamic responses of a PEM fuel-cell module. The accuracy of the model is verified by comparing the simulation and experimental results. Thirdly, a DSP-controlled three-phase induction-motor drive using constant voltage over frequency is built and can be used in a fuel-cell automobile. A hydrogen sensor is used in the drive to both sound an alarm and shut down the inverter trigger pulses through the DSP. Finally, a hybrid power system consisting of PV panels and fuel cell is proposed and built. In the proposed system, PV panels can supply most of the power when the sunlight is available, and the excess power required by the load is supplied by a fuel cell. Load sharing between a fuel cell (FC) and the PV panel is investigated by both simulation and experiments.

Protein Kinase-A Inhibition Is Sufficient to Support Human Neural Stem Cells Self-Renewal.

Science.gov (United States)

Georges, Pauline; Boissart, Claire; Poulet, Aurélie; Peschanski, Marc; Benchoua, Alexandra

2015-12-01

Human pluripotent stem cell-derived neural stem cells offer unprecedented opportunities for producing specific types of neurons for several biomedical applications. However, to achieve it, protocols of production and amplification of human neural stem cells need to be standardized, cost effective, and safe. This means that small molecules should progressively replace the use of media containing cocktails of protein-based growth factors. Here we have conducted a phenotypical screening to identify pathways involved in the regulation of hNSC self-renewal. We analyzed 80 small molecules acting as kinase inhibitors and identified compounds of the 5-isoquinolinesulfonamide family, described as protein kinase A (PKA) and protein kinase G inhibitors, as candidates to support hNSC self-renewal. Investigating the mode of action of these compounds, we found that modulation of PKA activity was central in controlling the choice between self-renewal or terminal neuronal differentiation of hNSC. We finally demonstrated that the pharmacological inhibition of PKA using the small molecule HA1004 was sufficient to support the full derivation, propagation, and long-term maintenance of stable hNSC in absence of any other extrinsic signals. Our results indicated that tuning of PKA activity is a core mechanism regulating hNSC self-renewal and differentiation and delineate the minimal culture media requirement to maintain undifferentiated hNSC in vitro. © 2015 AlphaMed Press.

System effects of nuclear energy and renewables in low-carbon electricity Systems

International Nuclear Information System (INIS)

Keppler, J.H.; Gameron, R.; Cometto, M.

2012-01-01

Electricity produced by variable renewable energies significantly affects the economics of dispatchable power generators, in particular those of nuclear power, both in the short run and the long run; the outcome of these competing factors will depend on the amount of variable renewables being introduced, local conditions and the level of carbon prices. An assessment of grid-level system costs (including the costs for grid connection, extension and reinforcement, as well as the added costs for balancing and back-up, but excluding the financial costs of intermittency and the impacts on security of supply, the environment, siting and safety), reveals a considerable difference between those of dispatchable technologies and those of variable renewables. Using a common methodology and a broad array of country-specific data, the grid-level system costs for Finland, France, Germany, the Republic of Korea, the United Kingdom and the United States were calculated for nuclear, coal, gas, onshore wind, offshore wind and solar PV both at 10 pc and 30 pc penetration levels. Variable renewables are creating a market environment in which dispatchable technologies can no longer finance themselves through revenues in 'energy only' wholesale markets; this has serious implications for the security of electricity supplies. Four main policy recommendations are proposed

Layered double hydroxide nanoparticles promote self-renewal of mouse embryonic stem cells through the PI3K signaling pathway

Science.gov (United States)

Wu, Youjun; Zhu, Rongrong; Zhou, Yang; Zhang, Jun; Wang, Wenrui; Sun, Xiaoyu; Wu, Xianzheng; Cheng, Liming; Zhang, Jing; Wang, Shilong

2015-06-01

Embryonic stem cells (ESCs) hold great potential for regenerative medicine due to their two unique characteristics: self-renewal and pluripotency. Several groups of nanoparticles have shown promising applications in directing the stem cell fate. Herein, we investigated the cellular effects of layered double hydroxide nanoparticles (LDH NPs) on mouse ESCs (mESCs) and the associated molecular mechanisms. Mg-Al-LDH NPs with an average diameter of ~100 nm were prepared by hydrothermal methods. To determine the influences of LDH NPs on mESCs, cellular cytotoxicity, self-renewal, differentiation potential, and the possible signaling pathways were explored. Evaluation of cell viability, lactate dehydrogenase release, ROS generation and apoptosis demonstrated the low cytotoxicity of LDH NPs. The alkaline phosphatase activity and the expression of pluripotency genes in mESCs were examined, which indicated that exposure to LDH NPs could support self-renewal and inhibit spontaneous differentiation of mESCs under feeder-free culture conditions. The self-renewal promotion was further proved to be independent of the leukemia inhibitory factor (LIF). Furthermore, cells treated with LDH NPs maintained the potential to differentiate into all three germ layers both in vitro and in vivo through formation of embryoid bodies and teratomas. In addition, we observed that LDH NPs initiated the activation of the PI3K/Akt pathway, while treatment with the PI3K inhibitor LY294002 could block the effects of LDH NPs on mESCs. The results confirmed that the promotion of self-renewal by LDH NPs was associated with activation of the PI3K/Akt signaling pathway. Altogether, our studies identified a new role of LDH NPs in maintaining self-renewal of mouse ES cells which could potentially be applied in stem cell research.Embryonic stem cells (ESCs) hold great potential for regenerative medicine due to their two unique characteristics: self-renewal and pluripotency. Several groups of nanoparticles

SirT1—A Sensor for Monitoring Self-Renewal and Aging Process in Retinal Stem Cells

Directory of Open Access Journals (Sweden)

Chi-Hsien Peng

2010-06-01

Full Text Available Retinal stem cells bear potency of proliferation, self-renewal, and differentiation into many retinal cells. Utilizing appropriate sensors one can effectively detect the self-renewal and aging process abilities. Silencing information regulator (SirT1, a member of the sirtuin family, is a NAD-dependent histone deacetylase and an essential mediator for longevity in normal cells by calorie restriction. We firstly investigate the SirT1 mRNA expression in retinal stem cells from rats and 19 human eyes of different ages. Results revealed that SirT1 expression was significantly decreased in in vivo aged eyes, associated with poor self-renewal abilities. Additionally, SirT1 mRNA levels were dose-dependently increased in resveratrol- treated retinal stem cells. The expression of SirT1 on oxidative stress-induced damage was significantly decreased, negatively correlated with the level of intracellular reactive oxygen species production. Treatment with resveratrol could effectively further reduce oxidative stress induced by H2O2 treatment in retinal stem cells. Importantly, the anti-oxidant effects of resveratrol in H2O2-treated retinal stem cells were significantly abolished by knockdown of SirT1 expression (sh-SirT1. SirT1 expression provides a feasible sensor in assessing self-renewal and aging process in retinal stem cells. Resveratrol can prevent reactive oxygen species-induced damages via increased retinal SirT1 expression.

Forecasting US renewables in the national energy modelling system

International Nuclear Information System (INIS)

Diedrich, R.; Petersik, T.W.

2001-01-01

The Energy information Administration (EIA) of the US Department of Energy (DOE) forecasts US renewable energy supply and demand in the context of overall energy markets using the National Energy Modelling System (NEMS). Renewables compete with other supply and demand options within the residential, commercial, industrial, transportation, and electricity sectors of the US economy. NEMS forecasts renewable energy for grid-connected electricity production within the Electricity Market Module (EM), and characterizes central station biomass, geothermal, conventional hydroelectric, municipal solid waste, solar thermal, solar photovoltaic, and wind-powered electricity generating technologies. EIA's Annual Energy Outlook 1998, projecting US energy markets, forecasts marketed renewables to remain a minor part of US energy production and consumption through to 2020. The USA is expected to remain primarily a fossil energy producer and consumer throughout the period. An alternative case indicates that biomass, wind, and to some extent geothermal power would likely increase most rapidly if the US were to require greater use of renewables for power supply, though electricity prices would increase somewhat. (author)

Power fluctuation reduction methodology for the grid-connected renewable power systems

Science.gov (United States)

Aula, Fadhil T.; Lee, Samuel C.

2013-04-01

This paper presents a new methodology for eliminating the influence of the power fluctuations of the renewable power systems. The renewable energy, which is to be considered an uncertain and uncontrollable resource, can only provide irregular electrical power to the power grid. This irregularity creates fluctuations of the generated power from the renewable power systems. These fluctuations cause instability to the power system and influence the operation of conventional power plants. Overall, the power system is vulnerable to collapse if necessary actions are not taken to reduce the impact of these fluctuations. This methodology aims at reducing these fluctuations and makes the generated power capability for covering the power consumption. This requires a prediction tool for estimating the generated power in advance to provide the range and the time of occurrence of the fluctuations. Since most of the renewable energies are weather based, as a result a weather forecast technique will be used for predicting the generated power. The reduction of the fluctuation also requires stabilizing facilities to maintain the output power at a desired level. In this study, a wind farm and a photovoltaic array as renewable power systems and a pumped-storage and batteries as stabilizing facilities are used, since they are best suitable for compensating the fluctuations of these types of power suppliers. As an illustrative example, a model of wind and photovoltaic power systems with battery energy and pumped hydro storage facilities for power fluctuation reduction is included, and its power fluctuation reduction is verified through simulation.

The B-MYB transcriptional network guides cell cycle progression and fate decisions to sustain self-renewal and the identity of pluripotent stem cells.

Science.gov (United States)

Zhan, Ming; Riordon, Daniel R; Yan, Bin; Tarasova, Yelena S; Bruweleit, Sarah; Tarasov, Kirill V; Li, Ronald A; Wersto, Robert P; Boheler, Kenneth R

2012-01-01

Embryonic stem cells (ESCs) are pluripotent and have unlimited self-renewal capacity. Although pluripotency and differentiation have been examined extensively, the mechanisms responsible for self-renewal are poorly understood and are believed to involve an unusual cell cycle, epigenetic regulators and pluripotency-promoting transcription factors. Here we show that B-MYB, a cell cycle regulated phosphoprotein and transcription factor critical to the formation of inner cell mass, is central to the transcriptional and co-regulatory networks that sustain normal cell cycle progression and self-renewal properties of ESCs. Phenotypically, B-MYB is robustly expressed in ESCs and induced pluripotent stem cells (iPSCs), and it is present predominantly in a hypo-phosphorylated state. Knockdown of B-MYB results in functional cell cycle abnormalities that involve S, G2 and M phases, and reduced expression of critical cell cycle regulators like ccnb1 and plk1. By conducting gene expression profiling on control and B-MYB deficient cells, ChIP-chip experiments, and integrative computational analyses, we unraveled a highly complex B-MYB-mediated transcriptional network that guides ESC self-renewal. The network encompasses critical regulators of all cell cycle phases and epigenetic regulators, pluripotency transcription factors, and differentiation determinants. B-MYB along with E2F1 and c-MYC preferentially co-regulate cell cycle target genes. B-MYB also co-targets genes regulated by OCT4, SOX2 and NANOG that are significantly associated with stem cell differentiation, embryonic development, and epigenetic control. Moreover, loss of B-MYB leads to a breakdown of the transcriptional hierarchy present in ESCs. These results coupled with functional studies demonstrate that B-MYB not only controls and accelerates cell cycle progression in ESCs it contributes to fate decisions and maintenance of pluripotent stem cell identity.

Integrated electrofuels and renewable energy systems

DEFF Research Database (Denmark)

Ridjan, Iva

energy into chemical energy by means of electrolysers, thus connecting fluctuating renewable energy to the vast amount of fuel storage already available in today’s energy systems. The conducted research indicates that electrofuels for heavy-duty transportation are technically and economically viable...... in energy systems and could play an important role in future energy systems. The cross-sector approach in the fuel production, by redirecting the excess electricity to the transport sector, is creating the flexibility and storage buffer for fluctuating electricity. The key concern in the short term should...

The p53 inhibitor, pifithrin-α, suppresses self-renewal of embryonic stem cells

International Nuclear Information System (INIS)

Abdelalim, Essam Mohamed; Tooyama, Ikuo

2012-01-01

Highlights: ► We determine the role of p53 in ES cells under unstressful conditions. ► PFT-α suppresses ES cell proliferation. ► PFT-α induces ES cell cycle arrest. ► PFT-α downregulates Nanog and cyclin D1. -- Abstract: Recent studies have reported the role of p53 in suppressing the pluripotency of embryonic stem (ES) cells after DNA damage and blocking the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. However, to date no evidence has been presented to support the function of p53 in unstressed ES cells. In this study, we investigated the effect of pifithrin (PFT)-α, an inhibitor of p53-dependent transcriptional activation, on self-renewal of ES cells. Our results revealed that treatment of ES cells with PFT-α resulted in the inhibition of ES cell propagation in a dose-dependent manner, as indicated by a marked reduction in the cell number and colony size. Also, PFT-α caused a cell cycle arrest and significant reduction in DNA synthesis. In addition, inhibition of p53 activity reduced the expression levels of cyclin D1 and Nanog. These findings indicate that p53 pathway in ES cells rather than acting as an inactive gene, is required for ES cell proliferation and self-renewal under unstressful conditions.

The p53 inhibitor, pifithrin-{alpha}, suppresses self-renewal of embryonic stem cells

Energy Technology Data Exchange (ETDEWEB)

Abdelalim, Essam Mohamed, E-mail: essam_abdelalim@yahoo.com [Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192 (Japan); Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522 (Egypt); Tooyama, Ikuo [Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192 (Japan)

2012-04-13

Highlights: Black-Right-Pointing-Pointer We determine the role of p53 in ES cells under unstressful conditions. Black-Right-Pointing-Pointer PFT-{alpha} suppresses ES cell proliferation. Black-Right-Pointing-Pointer PFT-{alpha} induces ES cell cycle arrest. Black-Right-Pointing-Pointer PFT-{alpha} downregulates Nanog and cyclin D1. -- Abstract: Recent studies have reported the role of p53 in suppressing the pluripotency of embryonic stem (ES) cells after DNA damage and blocking the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. However, to date no evidence has been presented to support the function of p53 in unstressed ES cells. In this study, we investigated the effect of pifithrin (PFT)-{alpha}, an inhibitor of p53-dependent transcriptional activation, on self-renewal of ES cells. Our results revealed that treatment of ES cells with PFT-{alpha} resulted in the inhibition of ES cell propagation in a dose-dependent manner, as indicated by a marked reduction in the cell number and colony size. Also, PFT-{alpha} caused a cell cycle arrest and significant reduction in DNA synthesis. In addition, inhibition of p53 activity reduced the expression levels of cyclin D1 and Nanog. These findings indicate that p53 pathway in ES cells rather than acting as an inactive gene, is required for ES cell proliferation and self-renewal under unstressful conditions.

Impacts of Renewable Energy Quota System on China's Future Power Sector

DEFF Research Database (Denmark)

Xiong, Weiming; Zhang, Da; Mischke, Peggy

2014-01-01

As the biggest carbon emitting sector which produces 44% of current national carbon emission in China, the coal-dominated power sector has a tremendous potential for CO2 mitigation in the next two decades. Renewable energy quota system is currently discussed as a potential future policy instrument...... for the power sector, which requires certain fraction of renewable energy in total power generation for each province and grid zone. The quantitative studies on renewable energy quota for China are still very limited. Based on a least-cost and technology-rich power generation and transmission expansion model...... for China, this study examines the impacts of renewable energy quota system and carbon cap policy instruments on the future Chinese power sector. Various scenarios are examined toward 2030 and their future power generation mix, capacity installations and carbon emission are discussed. This study concludes...

Smart Energy Systems for coherent 100% renewable energy and transport solutions

DEFF Research Database (Denmark)

Mathiesen, Brian Vad; Lund, Henrik; Connolly, David

2015-01-01

The hypothesis of this paper is that in order to identify least cost solutions of the integration of fluctuating renewable energy sources into current or future 100% renewable energy supplies one has to take a Smart Energy Systems approach. This paper outline why and how to do so. Traditionally......, significant focus is put on the electricity sector alone to solve the renewable energy integration puzzle. Smart grid research traditionally focuses on ICT, smart meters, electricity storage technologies, and local (electric) smart grids. In contrast, the Smart Energy System focuses on merging the electricity......, heating and transport sectors, in combination with various intra-hour, hourly, daily, seasonal and biannual storage options, to create the flexibility necessary to integrate large penetrations of fluctuating renewable energy. However, in this paper we present the development and design of coherent Smart...

Characterization of Hybrid Systems for Rural Electrification with Renewable Energies Using Geographic Information Systems (GIS)

Energy Technology Data Exchange (ETDEWEB)

Borda Angel, J. P.; Dominguez, J.; Amador, J.; Arribas, L.; Pinedo Pascua, I.

2011-07-01

The objective of this project is to redefine the algorithm of wind-diesel hybrid system implemented in IntiGIS. This methodology was developed by CIEMAT for the evaluation of rural electrification projects, comparing different renewable and conventional technologies based on their LEC or equivalent electrification cost. The analysis considers the social and geographical particularities of the study area. The core of the new model is the definition of renewable fraction in the wind-diesel hybrid system. To this end, it was assumed that the fraction of renewable will depend, first of all, of the wind speed. In this case, the objectives were to find a relationship between the renewable fraction and wind speed, expressed as a function, and also trying to demonstrate the influence of other parameters such as fuel price and consumption. The methodology used to achieve these objectives was to use HOMER to simulate technology and size of system components in order to obtain the optimal fraction renewable scenarios. Next, we examined how it varied with wind speed; we assessed the influence of other parameters and, finally, it is represented as a function of wind speed. After the redefinition of the algorithm, the changes were planned for inclusion in IntiGIS and tests were performed to validate the new model. (Author)

Hmga2 regulates self-renewal of retinal progenitors.

Science.gov (United States)

Parameswaran, Sowmya; Xia, Xiaohuan; Hegde, Ganapati; Ahmad, Iqbal

2014-11-01

In vertebrate retina, histogenesis occurs over an extended period. To sustain the temporal generation of diverse cell types, retinal progenitor cells (RPCs) must self-renew. However, self-renewal and regulation of RPCs remain poorly understood. Here, we demonstrate that cell-extrinsic factors coordinate with the epigenetic regulator high-mobility group AT-hook 2 (Hmga2) to regulate self-renewal of late retinal progenitor cells (RPCs). We observed that a small subset of RPCs was capable of clonal propagation and retained multipotentiality of parents in the presence of endothelial cells (ECs), known self-renewal regulators in various stem cell niches. The self-renewing effects, also observed in vivo, involve multiple intercellular signaling pathways, engaging Hmga2. As progenitors exhaust during retinal development, expression of Hmga2 progressively decreases. Analyses of Hmga2-expression perturbation, in vitro and in vivo, revealed that Hmga2 functionally helps to mediate cell-extrinsic influences on late-retinal progenitor self-renewal. Our results provide a framework for integrating the diverse intercellular influences elicited by epigenetic regulators for self-renewal in a dynamic stem cell niche: the developing vertebrate retina. © 2014. Published by The Company of Biologists Ltd.

Post-disaster resilience of a 100% renewable energy system in Japan

International Nuclear Information System (INIS)

Esteban, Miguel; Portugal-Pereira, Joana

2014-01-01

Following the 2011 Fukushima nuclear disaster, Japan is having to re-design its energy policy. With the danger of nuclear power in an earthquake-prone country exposed, renewable energies are being seen as a potential alternative. An assessment of the feasibility of a 100% renewable energy electricity system in Japan by the year 2030 was shown to be able to achieve a higher level of electricity resilience. The assessment is based on a simulation of the hourly future electricity production based on wind and solar meteorological data, that can cope with the estimated future hourly electricity demand in Japan for the year 2030. Such as system would use pump-up storage and electric batteries to balance the daily fluctuations in supply and demand, though the most important challenge of the system would be providing sufficient electricity to meet the summer demand peak. These findings have import implications at the policy making level, as it shows that the Japanese electricity generation system is technically able to increase the share of renewables up to 100%, guaranteeing a stable and reliable supply. - Highlights: • Hourly simulation of a 100% renewable energy system in Japan. • Feasibility analysis of system reliability and risk of failure. • Use of car electric batteries to store electricity during night. • Analysis of policy implications to implement decentralized smart grid system

Earmuff restricts progenitor cell potential by attenuating the competence to respond to self-renewal factors.

Science.gov (United States)

Janssens, Derek H; Komori, Hideyuki; Grbac, Daniel; Chen, Keng; Koe, Chwee Tat; Wang, Hongyan; Lee, Cheng-Yu

2014-03-01

Despite expressing stem cell self-renewal factors, intermediate progenitor cells possess restricted developmental potential, which allows them to give rise exclusively to differentiated progeny rather than stem cell progeny. Failure to restrict the developmental potential can allow intermediate progenitor cells to revert into aberrant stem cells that might contribute to tumorigenesis. Insight into stable restriction of the developmental potential in intermediate progenitor cells could improve our understanding of the development and growth of tumors, but the mechanisms involved remain largely unknown. Intermediate neural progenitors (INPs), generated by type II neural stem cells (neuroblasts) in fly larval brains, provide an in vivo model for investigating the mechanisms that stably restrict the developmental potential of intermediate progenitor cells. Here, we report that the transcriptional repressor protein Earmuff (Erm) functions temporally after Brain tumor (Brat) and Numb to restrict the developmental potential of uncommitted (immature) INPs. Consistently, endogenous Erm is detected in immature INPs but undetectable in INPs. Erm-dependent restriction of the developmental potential in immature INPs leads to attenuated competence to respond to all known neuroblast self-renewal factors in INPs. We also identified that the BAP chromatin-remodeling complex probably functions cooperatively with Erm to restrict the developmental potential of immature INPs. Together, these data led us to conclude that the Erm-BAP-dependent mechanism stably restricts the developmental potential of immature INPs by attenuating their genomic responses to stem cell self-renewal factors. We propose that restriction of developmental potential by the Erm-BAP-dependent mechanism functionally distinguishes intermediate progenitor cells from stem cells, ensuring the generation of differentiated cells and preventing the formation of progenitor cell-derived tumor-initiating stem cells.

Yap1 is dispensable for self-renewal but required for proper differentiation of mouse embryonic stem (ES) cells.

Science.gov (United States)

Chung, HaeWon; Lee, Bum-Kyu; Uprety, Nadima; Shen, Wenwen; Lee, Jiwoon; Kim, Jonghwan

2016-04-01

Yap1 is a transcriptional co-activator of the Hippo pathway. The importance of Yap1 in early cell fate decision during embryogenesis has been well established, though its role in embryonic stem (ES) cells remains elusive. Here, we report that Yap1 plays crucial roles in normal differentiation rather than self-renewal of ES cells. Yap1-depleted ES cells maintain undifferentiated state with a typical colony morphology as well as robust alkaline phosphatase activity. These cells also retain comparable levels of the core pluripotent factors, such as Pou5f1 and Sox2, to the levels in wild-type ES cells without significant alteration of lineage-specific marker genes. Conversely, overexpression of Yap1 in ES cells promotes nuclear translocation of Yap1, resulting in disruption of self-renewal and triggering differentiation by up-regulating lineage-specific genes. Moreover, Yap1-deficient ES cells show impaired induction of lineage markers during differentiation. Collectively, our data demonstrate that Yap1 is a required factor for proper differentiation of mouse ES cells, while remaining dispensable for self-renewal. © 2016 The Authors.

Power quality enhancement of renewable energy source power network using SMES system

International Nuclear Information System (INIS)

Seo, H.R.; Kim, A.R.; Park, M.; Yu, I.K.

2011-01-01

Power quality enhancement of a renewable energy source power network is performed by a real-toroidal-type SMES coil. SMES unit charges and discharges the HTS coil to mitigate the fluctuation of PV system output power. The grid connected PV and SMES system has been modeled and simulated using power-hard-in-the-loop simulation. The PHILS results demonstrated the effectiveness of the SMES system for enhancing power quality. This paper deals with power quality enhancement of renewable energy source power network using SMES system and describes the operation characteristics of HTS SMES system using real-toroidal-type SMES coil for smoothening the fluctuation of large-scale renewable energy source such as photovoltaic (PV) power generation system. It generates maximum power of PV array under various weather conditions. SMES unit charges and discharges the HTS coil to mitigate the fluctuation of PV system output power. The SMES unit is controlled according to the PV array output and the utility power quality conditions. The grid connected PV and SMES system has been modeled and simulated using power-hard-in-the-loop simulation (PHILS). The PHILS results demonstrated the effectiveness of the SMES system for enhancing power quality in power network including large-scale renewable energy source, especially PV power generation system.

Energy–exergy and economic analyses of a hybrid solar–hydrogen renewable energy system in Ankara, Turkey

International Nuclear Information System (INIS)

Ozden, Ender; Tari, Ilker

2016-01-01

Highlights: • Uninterrupted energy in an emergency blackout situation. • System modeling of a solar–hydrogen based hybrid renewable energy system. • A comprehensive thermodynamical analysis. • Levelized cost of electricity analysis for a project lifetime of 25 years. - Abstract: A hybrid (Solar–Hydrogen) stand-alone renewable energy system that consists of photovoltaic panels (PV), Proton Exchange Membrane (PEM) fuel cells, PEM based electrolyzers and hydrogen storage is investigated by developing a complete model of the system using TRNSYS. The PV panels are mounted on a tiltable platform to improve the performance of the system by monthly adjustments of the tilt angle. The total area of the PV panels is 300 m 2 , the PEM fuel cell capacity is 5 kW, and the hydrogen storage is at 55 bars pressure and with 45 m 3 capacity. The main goal of this study is to verify that the system meets the electrical power demand of the emergency room without experiencing a shortage for a complete year in an emergency blackout situation. For this purpose, after modeling the system, energy and exergy analyses for the hydrogen cycle of the system for a complete year are performed, and the energy and exergy efficiencies are found as 4.06% and 4.25%, respectively. Furthermore, an economic analysis is performed for a project lifetime of 25 years based on Levelized Cost of Electricity (LCE), and the LCE is calculated as 0.626 $/kWh.

Policies for 100% Renewable Energy Systems

DEFF Research Database (Denmark)

Hvelplund, Frede

2014-01-01

The official Danish energy policy goal is both to increase the wind power share of electricity consumption from 33% in 2014 to 50% by 2020 and to have a 100% renewable energy based energy system by 2050. This is a huge technological change from stored, scarce and polluting fossil fuels...... to fluctuating, abundant and clean energy sources. “Stored” fossil fuels can be used when needed; fluctuating energy sources must be captured when available and transformed to meet the energy needs of society in the right amounts and at the right time. We are amidst this change. Renewable energy has come of age...... and is no longer a minor technology experimenting in the corner of the energy scene, but has become a large new technology taking away considerable market shares from the old fossil fuel technologies....

An Optimization Framework for Investment Evaluation of Complex Renewable Energy Systems

Directory of Open Access Journals (Sweden)

David Olave-Rojas

2017-07-01

Full Text Available Enhancing the role of renewable energies in existing power systems is one of the most crucial challenges that society faces today. However, the high variability of their generation potential and the temporal disparity between the demand and the generation potential represent technological and operational gaps that burden the massive incorporation of renewable sources into power systems. Energy storage technologies are an alternative to tackle this gap; nonetheless, their incorporation within large-scale power grids calls for decision-making tools that ensure an appropriate design and sizing of power systems that exploit the benefits of incorporating storage facilities along with renewable generation power. In this paper, we present an optimization framework for aiding the evaluation of the strategic design of complex renewable power systems. The developed tool relies on an optimization problem, the generation, transmission, storage energy location and sizing problem, which allows one to compute economically-attractive investment plans given by the location and sizing of generation and storage energy systems, along with the corresponding layout of transmission lines. Results on a real case study (located in the central region of Chile, characterized by carefully-curated data, show the potential of the developed tool for aiding long-term investment planning.

Erk signaling suppresses embryonic stem cell self-renewal to specify endoderm

DEFF Research Database (Denmark)

Hamilton, William B; Brickman, Joshua M

2014-01-01

Fgf signaling via Erk activation has been associated with both neural induction and the generation of a primed state for the differentiation of embryonic stem cells (ESCs) to all somatic lineages. To dissect the role of Erk in both ESC self-renewal and lineage specification, we explored...

Energy Production System Management - Renewable energy power supply integration with Building Automation System

International Nuclear Information System (INIS)

Figueiredo, Joao; Martins, Joao

2010-01-01

Intelligent buildings, historically and technologically, refers to the integration of four distinctive systems: Building Automation Systems (BAS), Telecommunication Systems, Office Automation Systems and Computer Building Management Systems. The increasing sophisticated BAS has become the 'heart and soul' of modern intelligent buildings. Integrating energy supply and demand elements - often known as Demand-Side Management (DSM) - has became an important energy efficiency policy concept. Nowadays, European countries have diversified their power supplies, reducing the dependence on OPEC, and developing a broader mix of energy sources maximizing the use of renewable energy domestic sources. In this way it makes sense to include a fifth system into the intelligent building group: Energy Production System Management (EPSM). This paper presents a Building Automation System where the Demand-Side Management is fully integrated with the building's Energy Production System, which incorporates a complete set of renewable energy production and storage systems.

Optimal Integration of Intermittent Renewables: A System LCOE Stochastic Approach

Directory of Open Access Journals (Sweden)

Carlo Lucheroni

2018-03-01

Full Text Available We propose a system level approach to value the impact on costs of the integration of intermittent renewable generation in a power system, based on expected breakeven cost and breakeven cost risk. To do this, we carefully reconsider the definition of Levelized Cost of Electricity (LCOE when extended to non-dispatchable generation, by examining extra costs and gains originated by the costly management of random power injections. We are thus lead to define a ‘system LCOE’ as a system dependent LCOE that takes properly into account intermittent generation. In order to include breakeven cost risk we further extend this deterministic approach to a stochastic setting, by introducing a ‘stochastic system LCOE’. This extension allows us to discuss the optimal integration of intermittent renewables from a broad, system level point of view. This paper thus aims to provide power producers and policy makers with a new methodological scheme, still based on the LCOE but which updates this valuation technique to current energy system configurations characterized by a large share of non-dispatchable production. Quantifying and optimizing the impact of intermittent renewables integration on power system costs, risk and CO 2 emissions, the proposed methodology can be used as powerful tool of analysis for assessing environmental and energy policies.

miR-544 Regulates Dairy Goat Male Germline Stem Cell Self-Renewal via Targeting PLZF.

Science.gov (United States)

Song, Wencong; Mu, Hailong; Wu, Jiang; Liao, Mingzhi; Zhu, Haijing; Zheng, Liming; He, Xin; Niu, Bowen; Zhai, Yuanxin; Bai, Chunling; Lei, Anmin; Li, Guangpeng; Hua, Jinlian

2015-10-01

The balance between the self-renewal and differentiation of male germline stem cells (mGSCs) is critical for the initiation and maintenance of mammalian spermatogenesis. The promyelocytic leukemia zinc finger (PLZF), a zinc finger protein, is a critical factor for maintaining the self-renewal of mGSCs, so, evaluation of the PLZF pathway in mGSCs may provide a deeper insight into mammalian spermatogenesis. miRNA was also an important regulating factor for the self-renewal and differentiation of mGSCs; however, there is currently no data indicating that which miRNA regulate the self-renewal and differentiation of mGSCs via PLZF. Here, we predicted the prospective miRNA targeting to PLZF using the online Bioinformatics database-Targetscan, and performed an analysis of the dual-luciferase recombinant vector, psiCHCEKTM-2-PLZF-3'UTR. miR-544 mimics (miR-544m), miR-544 inhibitors (miR-544i), Control (NC, scrambled oligonucleotides transfection), pPLZF-IRES2-EGFP or PLZF siRNA were transfected into mGSCs; the cells proliferation was evaluated by BRDU incorporation assay and flow cytometry, and the mGSC marker, GFRa1, PLZF, KIT, DAZL, and VASA expression were analyzed by RT-qPCR, immunofluorescence and Western blot. The results showed that miR-544 regulates dairy goat male germline stem cell self-renewal via targeting PLZF. Our study identifies a new regulatory pathway for PLZF and expands upon the PLZF regulatory network in mGSCs. © 2015 Wiley Periodicals, Inc.

RE-Europe, a large-scale dataset for modeling a highly renewable European electricity system

Science.gov (United States)

Jensen, Tue V.; Pinson, Pierre

2017-11-01

Future highly renewable energy systems will couple to complex weather and climate dynamics. This coupling is generally not captured in detail by the open models developed in the power and energy system communities, where such open models exist. To enable modeling such a future energy system, we describe a dedicated large-scale dataset for a renewable electric power system. The dataset combines a transmission network model, as well as information for generation and demand. Generation includes conventional generators with their technical and economic characteristics, as well as weather-driven forecasts and corresponding realizations for renewable energy generation for a period of 3 years. These may be scaled according to the envisioned degrees of renewable penetration in a future European energy system. The spatial coverage, completeness and resolution of this dataset, open the door to the evaluation, scaling analysis and replicability check of a wealth of proposals in, e.g., market design, network actor coordination and forecasting of renewable power generation.

RE-Europe, a large-scale dataset for modeling a highly renewable European electricity system.

Science.gov (United States)

Jensen, Tue V; Pinson, Pierre

2017-11-28

Future highly renewable energy systems will couple to complex weather and climate dynamics. This coupling is generally not captured in detail by the open models developed in the power and energy system communities, where such open models exist. To enable modeling such a future energy system, we describe a dedicated large-scale dataset for a renewable electric power system. The dataset combines a transmission network model, as well as information for generation and demand. Generation includes conventional generators with their technical and economic characteristics, as well as weather-driven forecasts and corresponding realizations for renewable energy generation for a period of 3 years. These may be scaled according to the envisioned degrees of renewable penetration in a future European energy system. The spatial coverage, completeness and resolution of this dataset, open the door to the evaluation, scaling analysis and replicability check of a wealth of proposals in, e.g., market design, network actor coordination and forecasting of renewable power generation.

Dual role of BMP signaling in the regulation of Drosophila intestinal stem cell self-renewal.

Science.gov (United States)

Tian, Aiguo; Jiang, Jin

2017-10-02

Many adult organs including Drosophila adult midguts rely on resident stem cells to replenish damaged cells during tissue homeostasis and regeneration. Previous studies have shown that, upon injury, intestinal stem cells (ISCs) in the midguts can increase proliferation and lineage differentiation to meet the demand for tissue repair. Our recent study has demonstrated that, in response to certain injury, midguts can expand ISC population size as an additional regenerative mechanism. We found that injury elicited by bleomycin feeding or bacterial infection increased the production of two BMP ligands (Dpp and Gbb) in enterocytes (ECs), leading to elevated BMP signaling in progenitor cells that drove an expansion of ISCs by promoting their symmetric self-renewing division. Interestingly, we also found that BMP signaling in ECs inhibits the production of Dpp and Gbb, and that this negative feedback mechanism is required to reset ISC pool size to the homeostatic state. Our findings suggest that BMP signaling exerts two opposing influences on stem cell activity depending on where it acts: BMP signaling in progenitor cells promotes ISC self-renewal while BMP signaling in ECs restricts ISC self-renewal by preventing excessive production of BMP ligands. Our results further suggest that transient expansion of ISC population in conjunction with increasing ISC proliferation provides a more effective strategy for tissue regeneration.

Experimental study of mini SCADA renewable energy management system on microgrid using Raspberry Pi

Science.gov (United States)

Tridianto, E.; Permatasari, P. D.; Ali, I. R.

2018-03-01

Renewable Energy Management System (REMS) is a device that can be able to monitor power through a microgrid. The purpose of this system is to optimize power usage that produced from renewable energy with the result that reduces power demand from the grid. To reach the goal this device manage the load power needs fully supplied by renewable energy when the power produced from renewable energy is higher than load demand, besides power surplus will be stored in battery in this way energy stored in battery can be used when it needed. When the power produced from renewable energy can not satisfy the power demand, power will supply by renewable energy and grid. This device uses power meters for record any power flow through microgrid. In order to manage power flow in microgrid this system use relay module. The user can find out energy consumption (consumed by the load) and production (produced by renewable energy) in a period of time so that the user can switch on the load in right time.

Ink4a and Arf differentially affect cell proliferation and neural stem cell self-renewal in Bmi1-deficient mice

NARCIS (Netherlands)

Bruggeman, SWM; Valk-Lingbeek, ME; van der Stoop, PPM; Jacobs, JJL; Kieboom, K; Tanger, E; Hulsman, D; Leung, C; Arsenijevic, Y; Marino, S; van Lohuizen, M

2005-01-01

The Polycomb group (PcG) gene Bmi1 promotes cell proliferation and stem cell self-renewal by repressing the Ink4a/Arf locus. We used a genetic approach to investigate whether Ink4a or Arf is more critical for relaying Bmi1 function in lymphoid cells, neural progenitors, and neural stem cells. We

SIMULATION OF THE SYSTEMS WITH RENEWABLE ENERGY SOURCES USING HOMER SOFTWARE

Directory of Open Access Journals (Sweden)

FIRINCĂ S.D.

2015-12-01

Full Text Available This paper simulates by using the Homer software, distributed energy systems with capacity below 1 MW. Among the renewable energy sources are used wind and solar energy. For photovoltaic panels, we are considering two situations: fixed panels, oriented at 45 ° and panels with tracking system with two axis. Simulation results contain information regarding operation hours of the system throughout the year, energy produced from the renewable energy sources, energy consumption for the load, and excess of electrical energy. The Homer software also allows an economic analysis of these systems.

NAPS renewable energy systems R and D

International Nuclear Information System (INIS)

Spiers, D.J.

1990-01-01

Neste Advanced Power Systems (NAPS) is a renewable energy systems company supplying complete power systems based on photovoltaics, wind turbine generator, diesel generators, or hybrid systems combining two or more of these. Except for a few demonstration systems linked to the electricity grid, these are stand-alone power supplies which include storage batteries. Our present market areas are: Domestic systems for remote houses, largely in the Nordic countries. Systems for developing countries, mostly for lighting, health care and water supply and industrial and professional systems for use anywhere in the world, mainly for telecommunications and navigation aids

ERK2 suppresses self-renewal capacity of embryonic stem cells, but is not required for multi-lineage commitment.

Directory of Open Access Journals (Sweden)

William B Hamilton

Full Text Available Activation of the FGF-ERK pathway is necessary for naïve mouse embryonic stem (ES cells to exit self-renewal and commit to early differentiated lineages. Here we show that genetic ablation of Erk2, the predominant ERK isozyme expressed in ES cells, results in hyper-phosphorylation of ERK1, but an overall decrease in total ERK activity as judged by substrate phosphorylation and immediate-early gene (IEG induction. Normal induction of this subset of canonical ERK targets, as well as p90RSK phosphorylation, was rescued by transgenic expression of either ERK1 or ERK2 indicating a degree of functional redundancy. In contrast to previously published work, Erk2-null ES cells exhibited no detectable defect in lineage specification to any of the three germ layers when induced to differentiate in either embryoid bodies or in defined neural induction conditions. However, under self-renewing conditions Erk2-null ES cells express increased levels of the pluripotency-associated transcripts, Nanog and Tbx3, a decrease in Nanog-GFP heterogeneity, and exhibit enhanced self-renewal in colony forming assays. Transgenic add-back of ERK2 is capable of restoring normal pluripotent gene expression and self-renewal capacity. We show that ERK2 contributes to the destabilization of ES cell self-renewal by reducing expression of pluripotency genes, such as Nanog, but is not specifically required for the early stages of germ layer specification.

Reliability assessment of distribution system with the integration of renewable distributed generation

International Nuclear Information System (INIS)

Adefarati, T.; Bansal, R.C.

2017-01-01

Highlights: • Addresses impacts of renewable DG on the reliability of the distribution system. • Multi-objective formulation for maximizing the cost saving with integration of DG. • Uses Markov model to study the stochastic characteristics of the major components. • The investigation is done using modified RBTS bus test distribution system. • Proposed approach is useful for electric utilities to enhance the reliability. - Abstract: Recent studies have shown that renewable energy resources will contribute substantially to future energy generation owing to the rapid depletion of fossil fuels. Wind and solar energy resources are major sources of renewable energy that have the ability to reduce the energy crisis and the greenhouse gases emitted by the conventional power plants. Reliability assessment is one of the key indicators to measure the impact of the renewable distributed generation (DG) units in the distribution networks and to minimize the cost that is associated with power outage. This paper presents a comprehensive reliability assessment of the distribution system that satisfies the consumer load requirements with the penetration of wind turbine generator (WTG), electric storage system (ESS) and photovoltaic (PV). A Markov model is proposed to access the stochastic characteristics of the major components of the renewable DG resources as well as their influence on the reliability of a conventional distribution system. The results obtained from the case studies have demonstrated the effectiveness of using WTG, ESS and PV to enhance the reliability of the conventional distribution system.

miR-99 regulates normal and malignant hematopoietic stem cell self-renewal.

Science.gov (United States)

Khalaj, Mona; Woolthuis, Carolien M; Hu, Wenhuo; Durham, Benjamin H; Chu, S Haihua; Qamar, Sarah; Armstrong, Scott A; Park, Christopher Y

2017-07-21

The microRNA-99 ( miR-99 ) family comprises a group of broadly conserved microRNAs that are highly expressed in hematopoietic stem cells (HSCs) and acute myeloid leukemia stem cells (LSCs) compared with their differentiated progeny. Herein, we show that miR-99 regulates self-renewal in both HSCs and LSCs. miR-99 maintains HSC long-term reconstitution activity by inhibiting differentiation and cell cycle entry. Moreover, miR-99 inhibition induced LSC differentiation and depletion in an MLL-AF9-driven mouse model of AML, leading to reduction in leukemia-initiating activity and improved survival in secondary transplants. Confirming miR-99 's role in established AML, miR-99 inhibition induced primary AML patient blasts to undergo differentiation. A forward genetic shRNA library screen revealed Hoxa1 as a critical mediator of miR-99 function in HSC maintenance, and this observation was independently confirmed in both HSCs and LSCs. Together, these studies demonstrate the importance of noncoding RNAs in the regulation of HSC and LSC function and identify miR-99 as a critical regulator of stem cell self-renewal. © 2017 Khalaj et al.

Renewal Processes and Repairable Systems

NARCIS (Netherlands)

2003-01-01

In this thesis we discuss the following topics: 1. Renewal reward processes The marginal distributions of renewal reward processes and its version, which we call in this thesis instantaneous reward processes, are derived. Our approach is based on the theory of point processes, especially Poisson

Impacts of Renewable Energy Quota System on China's Future Power Sector

OpenAIRE

Xiong, Weiming; Zhang, Da; Mischke, Peggy; Zhang, Xiliang

2014-01-01

As the biggest carbon emitting sector which produces 44% of current national carbon emission in China, the coal-dominated power sector has a tremendous potential for CO2 mitigation in the next two decades. Renewable energy quota system is currently discussed as a potential future policy instrument for the power sector, which requires certain fraction of renewable energy in total power generation for each province and grid zone. The quantitative studies on renewable energy quota for China are ...

Asymmetric segregation and self-renewal of hematopoietic stem and progenitor cells with endocytic Ap2a2.

Science.gov (United States)

Ting, Stephen B; Deneault, Eric; Hope, Kristin; Cellot, Sonia; Chagraoui, Jalila; Mayotte, Nadine; Dorn, Jonas F; Laverdure, Jean-Philippe; Harvey, Michael; Hawkins, Edwin D; Russell, Sarah M; Maddox, Paul S; Iscove, Norman N; Sauvageau, Guy

2012-03-15

The stem cell-intrinsic model of self-renewal via asymmetric cell division (ACD) posits that fate determinants be partitioned unequally between daughter cells to either activate or suppress the stemness state. ACD is a purported mechanism by which hematopoietic stem cells (HSCs) self-renew, but definitive evidence for this cellular process remains open to conjecture. To address this issue, we chose 73 candidate genes that function within the cell polarity network to identify potential determinants that may concomitantly alter HSC fate while also exhibiting asymmetric segregation at cell division. Initial gene-expression profiles of polarity candidates showed high and differential expression in both HSCs and leukemia stem cells. Altered HSC fate was assessed by our established in vitro to in vivo screen on a subcohort of candidate polarity genes, which revealed 6 novel positive regulators of HSC function: Ap2a2, Gpsm2, Tmod1, Kif3a, Racgap1, and Ccnb1. Interestingly, live-cell videomicroscopy of the endocytic protein AP2A2 shows instances of asymmetric segregation during HSC/progenitor cell cytokinesis. These results contribute further evidence that ACD is functional in HSC self-renewal, suggest a role for Ap2a2 in HSC activity, and provide a unique opportunity to prospectively analyze progeny from HSC asymmetric divisions.

Pleiotropy of Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Self-Renewal of Embryonic Stem Cells from Refractory Mouse Strains

Science.gov (United States)

Ye, Shoudong; Tan, Li; Yang, Rongqing; Fang, Bo; Qu, Su; Schulze, Eric N.; Song, Houyan; Ying, Qilong; Li, Ping

2012-01-01

Background Inhibition of glycogen synthase kinase-3 (GSK-3) improves the efficiency of embryonic stem (ES) cell derivation from various strains of mice and rats, as well as dramatically promotes ES cell self-renewal potential. β-catenin has been reported to be involved in the maintenance of self-renewal of ES cells through TCF dependent and independent pathway. But the intrinsic difference between ES cell lines from different species and strains has not been characterized. Here, we dissect the mechanism of GSK-3 inhibition by CHIR99021 in mouse ES cells from refractory mouse strains. Methodology/Principal Findings We found that CHIR99021, a GSK-3 specific inhibitor, promotes self-renewal of ES cells from recalcitrant C57BL/6 (B6) and BALB/c mouse strains through stabilization of β-catenin and c-Myc protein levels. Stabilized β-catenin promoted ES self-renewal through two mechanisms. First, β-catenin translocated into the nucleus to maintain stem cell pluripotency in a lymphoid-enhancing factor/T-cell factor–independent manner. Second, β-catenin binds plasma membrane-localized E-cadherin, which ensures a compact, spherical morphology, a hallmark of ES cells. Further, elevated c-Myc protein levels did not contribute significantly to CH-mediated ES cell self-renewal. Instead, the role of c-Myc is dependent on its transformation activity and can be replaced by N-Myc but not L-Myc. β-catenin and c-Myc have similar effects on ES cells derived from both B6 and BALB/c mice. Conclusions/Significance Our data demonstrated that GSK-3 inhibition by CH promotes self-renewal of mouse ES cells with non-permissive genetic backgrounds by regulation of multiple signaling pathways. These findings would be useful to improve the availability of normally non-permissive mouse strains as research tools. PMID:22540008

Pretreated Landfill Gas Conversion Process via a Catalytic Membrane Reactor for Renewable Combined Fuel Cell-Power Generation

Directory of Open Access Journals (Sweden)

Zoe Ziaka

2013-01-01

Full Text Available A new landfill gas-based reforming catalytic processing system for the conversion of gaseous hydrocarbons, such as incoming methane to hydrogen and carbon oxide mixtures, is described and analyzed. The exit synthesis gas (syn-gas is fed to power effectively high-temperature fuel cells such as SOFC types for combined efficient electricity generation. The current research work is also referred on the description and design aspects of permreactors (permeable reformers carrying the same type of landfill gas-reforming reactions. Membrane reactors is a new technology that can be applied efficiently in such systems. Membrane reactors seem to perform better than the nonmembrane traditional reactors. The aim of this research includes turnkey system and process development for the landfill-based power generation and fuel cell industries. Also, a discussion of the efficient utilization of landfill and waste type resources for combined green-type/renewable power generation with increased processing capacity and efficiency via fuel cell systems is taking place. Moreover, pollution reduction is an additional design consideration in the current catalytic processors fuel cell cycles.

Design, engineering, and construction of photosynthetic microbial cell factories for renewable solar fuel production.

Science.gov (United States)

Lindblad, Peter; Lindberg, Pia; Oliveira, Paulo; Stensjö, Karin; Heidorn, Thorsten

2012-01-01

There is an urgent need to develop sustainable solutions to convert solar energy into energy carriers used in the society. In addition to solar cells generating electricity, there are several options to generate solar fuels. This paper outlines and discusses the design and engineering of photosynthetic microbial systems for the generation of renewable solar fuels, with a focus on cyanobacteria. Cyanobacteria are prokaryotic microorganisms with the same type of photosynthesis as higher plants. Native and engineered cyanobacteria have been used by us and others as model systems to examine, demonstrate, and develop photobiological H(2) production. More recently, the production of carbon-containing solar fuels like ethanol, butanol, and isoprene have been demonstrated. We are using a synthetic biology approach to develop efficient photosynthetic microbial cell factories for direct generation of biofuels from solar energy. Present progress and advances in the design, engineering, and construction of such cyanobacterial cells for the generation of a portfolio of solar fuels, e.g., hydrogen, alcohols, and isoprene, are presented and discussed. Possibilities and challenges when introducing and using synthetic biology are highlighted.

Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production

Energy Technology Data Exchange (ETDEWEB)

Lindblad, Peter; Lindberg, Pia; Stensjoe, Karin (Photochemistry and Molecular Science, Dept. of Chemistry-Aangstroem Laboratory, Uppsala Univ., Uppsala (Sweden)), E-mail: Peter.Lindblad@kemi.uu.se; Oliveira, Paulo (Instituto de Biologia Molecular e Celular, Porto (Portugal)); Heidorn, Thorsten (Bioforsk-Norwegian Inst. for Agricultural and Environmental Research, Aas Oslo, (Norway))

2012-03-15

There is an urgent need to develop sustainable solutions to convert solar energy into energy carriers used in the society. In addition to solar cells generating electricity, there are several options to generate solar fuels. This paper outlines and discusses the design and engineering of photosynthetic microbial systems for the generation of renewable solar fuels, with a focus on cyanobacteria. Cyanobacteria are prokaryotic microorganisms with the same type of photosynthesis as higher plants. Native and engineered cyanobacteria have been used by us and others as model systems to examine, demonstrate, and develop photobiological H{sub 2} production. More recently, the production of carbon-containing solar fuels like ethanol, butanol, and isoprene have been demonstrated. We are using a synthetic biology approach to develop efficient photosynthetic microbial cell factories for direct generation of biofuels from solar energy. Present progress and advances in the design, engineering, and construction of such cyanobacterial cells for the generation of a portfolio of solar fuels, e.g., hydrogen, alcohols, and isoprene, are presented and discussed. Possibilities and challenges when introducing and using synthetic biology are highlighted

New perspectives on renewable energy systems based on hydrogen

International Nuclear Information System (INIS)

Bose, T. K.; Agbossou, K.; Benard, P.; St-Arnaud, J-M.

1999-01-01

Current hydrocarbon-based energy systems, current energy consumption and the push towards the utilization of renewable energy sources, fuelled by global warming and the need to reduce atmospheric pollution are discussed. The consequences of climatic change and the obligation of Annex B countries to reduce their greenhouse gas emissions in terms of the Kyoto Protocols are reviewed. The role that renewable energy sources such as hydrogen, solar and wind energy could play in avoiding the most catastrophic consequences of rapidly growing energy consumption and atmospheric pollution in the face of diminishing conventional fossil fuel resources are examined. The focus is on hydrogen energy as a means of storing and transporting primary energy. Some favorable characteristics of hydrogen is its abundance, the fact that it can be produced utilizing renewable or non-renewable sources, and the further fact that its combustion produces three times more energy per unit of mass than oil, and six times more than coal. The technology of converting hydrogen into energy, storing energy in the form of hydrogen, and its utilization, for example in the stabilization of wind energy by way of electrolytic conversion to hydrogen, are described. Development at Hydro-Quebec's Institute of Research of a hydrogen-based autonomous wind energy system to produce electricity is also discussed. 2 tabs., 11 refs

FOXP3 inhibits cancer stem cell self-renewal via transcriptional repression of COX2 in colorectal cancer cells.

Science.gov (United States)

Liu, Shuo; Zhang, Cun; Zhang, Kuo; Gao, Yuan; Wang, Zhaowei; Li, Xiaoju; Cheng, Guang; Wang, Shuning; Xue, Xiaochang; Li, Weina; Zhang, Wei; Zhang, Yingqi; Xing, Xianghui; Li, Meng; Hao, Qiang

2017-07-04

Colon cancer stem cell (cCSC) is considered as the seed cell of colon cancer initiation and metastasis. Cyclooxygenase-2 (COX2), a downstream target of NFκB, is found to be essential in promoting cancer stem cell renewal. However, how COX2 is dysregulated in cCSCs is largely unknown. In this study, we found that the expression of transcription factor FOXP3 was much lower in the spheroids than that in the parental tumor cells. Overexpression of FOXP3 significantly decreased the numbers of spheres, reduced the side population. Accordingly, FOXP3 expression decreased the tumor size and weight in the xenograft model. The tumor inhibitory effects of FOXP3 were rarely seen when COX2 was additionally knocked down. Mechanically, FOXP3 transcriptionally repressed COX2 expression via interacting with and thus inhibiting p65 activity on the putative NFκB response elements in COX2 promoter. Taken together, we here revealed possible involvement of FOXP3 in regulating cCSC self-renewal via tuning COX2 expression, and thus providing a new target for the eradication of colon cancer stem cells.

Adult hematopoietic stem cells lacking Hif-1α self-renew normally

Science.gov (United States)

Vukovic, Milica; Sepulveda, Catarina; Subramani, Chithra; Guitart, Amélie V.; Mohr, Jasmine; Allen, Lewis; Panagopoulou, Theano I.; Paris, Jasmin; Lawson, Hannah; Villacreces, Arnaud; Armesilla-Diaz, Alejandro; Gezer, Deniz; Holyoake, Tessa L.; Ratcliffe, Peter J.

2016-01-01

The hematopoietic stem cell (HSC) pool is maintained under hypoxic conditions within the bone marrow microenvironment. Cellular responses to hypoxia are largely mediated by the hypoxia-inducible factors, Hif-1 and Hif-2. The oxygen-regulated α subunits of Hif-1 and Hif-2 (namely, Hif-1α and Hif-2α) form dimers with their stably expressed β subunits and control the transcription of downstream hypoxia-responsive genes to facilitate adaptation to low oxygen tension. An initial study concluded that Hif-1α is essential for HSC maintenance, whereby Hif-1α–deficient HSCs lost their ability to self-renew in serial transplantation assays. In another study, we demonstrated that Hif-2α is dispensable for cell-autonomous HSC maintenance, both under steady-state conditions and following transplantation. Given these unexpected findings, we set out to revisit the role of Hif-1α in cell-autonomous HSC functions. Here we demonstrate that inducible acute deletion of Hif-1α has no impact on HSC survival. Notably, unstressed HSCs lacking Hif-1α efficiently self-renew and sustain long-term multilineage hematopoiesis upon serial transplantation. Finally, Hif-1α–deficient HSCs recover normally after hematopoietic injury induced by serial administration of 5-fluorouracil. We therefore conclude that despite the hypoxic nature of the bone marrow microenvironment, Hif-1α is dispensable for cell-autonomous HSC maintenance. PMID:27060169

IGF-1R Promotes Symmetric Self-Renewal and Migration of Alkaline Phosphatase+ Germ Stem Cells through HIF-2α-OCT4/CXCR4 Loop under Hypoxia

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Yung-Che Kuo

2018-02-01

Full Text Available Summary: Hypoxia cooperates with endocrine signaling to maintain the symmetric self-renewal proliferation and migration of embryonic germline stem cells (GSCs. However, the lack of an appropriate in vitro cell model has dramatically hindered the understanding of the mechanism underlying this cooperation. Here, using a serum-free system, we demonstrated that hypoxia significantly induced the GSC mesenchymal transition, increased the expression levels of the pluripotent transcription factor OCT4 and migration-associated proteins (SDF-1, CXCR4, IGF-1, and IGF-1R, and activated the cellular expression and translocalization of the CXCR4-downstream proteins ARP3/pFAK. The underlying mechanism involved significant IGF-1/IGF-1R activation of OCT4/CXCR4 expression through HIF-2α regulation. Picropodophyllin-induced inhibition of IGF-1R phosphorylation significantly suppressed hypoxia-induced SDF-1/CXCR4 expression and cell migration. Furthermore, transactivation between IGF-1R and CXCR4 was involved. In summary, we demonstrated that niche hypoxia synergistically cooperates with its associated IGF-1R signaling to regulate the symmetric division (self-renewal proliferation and cell migration of alkaline phosphatase-positive GSCs through HIF-2α-OCT4/CXCR4 during embryogenesis. : In this article, Huang and colleagues demonstrate that niche hypoxia promotes symmetric self-renewal proliferation and migration of PGC-like CD49f+AP+GSCs through IGF-IR regulation. Using a serum-free culture system, the crosstalk between IGF-1R and CXCR4 signaling was discovered. This work demonstrated that embryonic hypoxia synergistically cooperated with IGF-1R signaling to regulate the symmetric self-renewal and migration of PGC-like GSCs through a HIF-2α–OCT4/CXCR4 loop. Keywords: hypoxia, niche, germline stem cells, self-renewal, migration, IGF-1R, HIF-2α, OCT4, SDF-1, CXCR4

Pressure retarded osmosis as a controlling system for traditional renewables

Science.gov (United States)

Carravetta, Armando; Fecarotta, Oreste; La Rocca, Michele; Martino, Riccardo

2015-04-01

Pressure retarded osmosis (PRO) is a viable but still not diffused form of renewable energy (see Maisonneuve et al., 2015 for a recent literature review). In PRO, water from a low salinity feed solution permeates through a membrane into a pressurized, high salinity draw solution, giving rise to a positive pressure drop; then energy is obtained by depressurizing the permeate through a hydro-turbine and brackish water is discharged. Many technological, environmental and economical aspects are obstacles in the diffusion of PRO, like the vulnerability of the membranes to fouling, the impact of the brackish water on the local marine environment, the high cost of membranes, etc. We are interested in the use of PRO as a combined form of energy with other renewable energy source like solar, wind or mini hydro in water supply networks (WSN). For the wide diffusion of renewables one of the major concerns of commercial power companies is to obtain very stable form of energy to comply with prescriptions of electricity grid operators and with the instant energy demand curve. Renewables are generally very variable form of energy, for the influence of climatic conditions on available power, and of the fluctuation in water demand in WSN. PRO is a very flexible technology where with appropriate turbines and control system power can be varied continuously to compensate for variation of other source of energy. Therefore, PRO is suitable to be used as a balancing system for commercial power system. We will present a simulation of the performance of a PRO used in combination with three different renewables. In the first two scenarios PRO compensate the difference between energy demand and energy production of a solar power plant and hydro power plant in a WSN. In the third scenario PRO is used to compensate daily variation of energy production in a wind power plant. Standard curves of energy production and energy demand for southern Italy are used. In order to control PRO production an

Curtailment in a Highly Renewable Power System and Its Effect on Capacity Factors

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Alexander Kies

2016-06-01

Full Text Available The capacity factor of a power plant is the ratio of generation over its potential generation. It is an important measure to describe wind and solar resources. However, the fluctuating nature of renewable power generation makes it difficult to integrate all generation at times. Whenever generation exceeds the load, curtailment or storage of energy is required. With increasing renewable shares in the power system, the level of curtailment will further increase. In this work, the influence of the curtailment on the capacity factors for a highly renewable German power system is studied. An effective capacity factor is introduced, and the implications for the distribution of renewable power plants are discussed. Three years of highly-resolved weather data were used to model wind and solar power generation. Together with historical load data and a transmission model, a possible future German power system was simulated. It is shown that effective capacity factors for unlimited transmission are strongly reduced by up to 60% (wind and 70% (photovoltaics and therefore of limited value in a highly renewable power system. Furthermore, the results demonstrate that wind power benefits more strongly from a reinforced transmission grid than photovoltaics (PV does.

Power Electronics for Renewable Energy Systems - Status and Trends

DEFF Research Database (Denmark)

Blaabjerg, Frede; Ma, Ke; Yang, Yongheng

2014-01-01

electronics in generation, transmission/distribution and end-user application, together with advanced controls, can pave the way for renewable energy resources. In view of this, some of the most promising renewable candidates like wind power and photovoltaic, which are becoming a significant part...... in the electricity production, are explored in this paper. Issues like technology demands, power converter topologies, and control structures are addressed. Some special focuses are also paid on the emerging trends in power electronics development for those systems....

International Conference on Power Electronics and Renewable Energy Systems

CERN Document Server

Suresh, L; Dash, Subhransu; Panigrahi, Bijaya

2015-01-01

The book is a collection of high-quality peer-reviewed research papers presented in Proceedings of International Conference on Power Electronics and Renewable Energy Systems (ICPERES 2014) held at Rajalakshmi Engineering College, Chennai, India. These research papers provide the latest developments in the broad area of Power Electronics and Renewable Energy. The book discusses wide variety of industrial, engineering and scientific applications of the emerging techniques. It presents invited papers from the inventors/originators of new applications and advanced technologies.

Simplified Method of Optimal Sizing of a Renewable Energy Hybrid System for Schools

Directory of Open Access Journals (Sweden)

Jiyeon Kim

2016-11-01

Full Text Available Schools are a suitable public building for renewable energy systems. Renewable energy hybrid systems (REHSs have recently been introduced in schools following a new national regulation that mandates renewable energy utilization. An REHS combines the common renewable-energy sources such as geothermal heat pumps, solar collectors for water heating, and photovoltaic systems with conventional energy systems (i.e., boilers and air-source heat pumps. Optimal design of an REHS by adequate sizing is not a trivial task because it usually requires intensive work including detailed simulation and demand/supply analysis. This type of simulation-based approach for optimization is difficult to implement in practice. To address this, this paper proposes simplified sizing equations for renewable-energy systems of REHSs. A conventional optimization process is used to calculate the optimal combinations of an REHS for cases of different numbers of classrooms and budgets. On the basis of the results, simplified sizing equations that use only the number of classrooms as the input are proposed by regression analysis. A verification test was carried out using an initial conventional optimization process. The results show that the simplified sizing equations predict similar sizing results to the initial process, consequently showing similar capital costs within a 2% error.

The Ganil computer control system renewal

International Nuclear Information System (INIS)

David, L.; Lecorche, E.; Luong, T.T.; Ulrich, M.

1990-01-01

Since 1982 the GANIL heavy ion accelerator has been under the control of 16-bit minicomputers MITRA, programmable logic controllers and microprocessorized Camac controllers, structured into a partially centralized system. This control system has to be renewed to meet the increasing demands of the accelerator operation which aims to provide higher quality ion beams under more reliable conditions. This paper gives a brief description of the existing control system and then discusses the main issues of the design and the implementation of the future control system: distributed powerful processors federated through Ethernet and flexible network-wide database access, VME standard and front-end microprocessors, enhanced color graphic tools and workstation based operator interface

Nuclear-Renewable Hybrid Energy System Market Analysis Plans

Energy Technology Data Exchange (ETDEWEB)

Ruth, Mark

2016-06-09

This presentation describes nuclear-renewable hybrid energy systems (N-R HESs), states their potential benefits, provides figures for the four tightly coupled N-R HESs that NREL is currently analyzing, and outlines the analysis process that is underway.

Renewable technologies for generation systems in islands and their application to Cozumel Island, Mexico

OpenAIRE

Mendoza Vizcaino, Javier; Sumper, Andreas; Sudrià Andreu, Antoni; Ramirez, J.M.

2016-01-01

The electric generation systems on islands are based generally on fossil fuel. This fact and its supply make the electricity cost higher than in systems used in the continent. In this article, we present a review of the renewable energy generation systems on islands. To do it we analysed 77 islands from 45 different countries. This work will allow us to know how the implementation of renewable energy sources could help these islands in developing a renewable and sustainable energy sector, inc...

Renewable energy systems - the environmental impact approach. Paper no. IGEC-1-008

International Nuclear Information System (INIS)

Koroneos, C.

2005-01-01

High energy consumption and the world population increase will lead to a shrinking use of fossil fuels. The combustion of Fossil fuel leads to the increase of carbon dioxide concentration in the atmosphere which leads to the probable increase of global warming. Therefore, concerns about carbon dioxide emissions may discourage widespread dependence on fossil fuels and encourage the development and use of renewable energy systems employing a variety of technologies Renewable energy systems have themselves an environmental impact. Land use and material employed are two areas that may have an adverse impact to the positive environmental picture of the renewable energy systems. The objective of this paper is to analyze these impacts with the use of a very powerful tool, the Life Cycle Assessment. (author)

Enhanced Hematopoietic Stem Cell Self-Renewal-Promoting Ability of Clonal Primary Mesenchymal Stromal/Stem cells Versus Their Osteogenic Progeny.

Science.gov (United States)

He, Qiling; Scott Swindle, Claude; Wan, Chao; Flynn, Robert J; Oster, Robert A; Chen, Dongquan; Zhang, Fengjie; Shu, Yinglan; Klug, Christopher A

2017-02-01

Long-term self-renewing hematopoietic stem cell (LT-HSC) homeostasis within the bone marrow (BM) of adult mammals is regulated by complex interactions between LT-HSC and a number of niche-associated cell types including mesenchymal stromal/stem cells (MSC), osteoblasts (OB), macrophage, and neuronal cells in close proximity with the vasculature. Here, we cloned and functionally characterized a murine BM MSC subpopulation that was uniformly Nestin + Lepr + Sca-1 + CD146 + and could be stably propagated with high colony-forming unit fibroblast re-cloning efficiency. MSC synergized with SCF and IL-11 to support a 20-fold expansion in true LT-HSC after 10-days of in vitro coculture. Optimal stimulation of LT-HSC expansion was minimally dependent on Notch signaling but was significantly enhanced by global inhibition of Wnt signaling. The self-renewal-promoting activity of MSC was progressively lost when MSC clones were differentiated into mature OB. This suggests that the stage of osteoblast development may significantly impact the ability of osteolineage cells to support LT-HSC homeostasis in vivo. Stem Cells 2017;35:473-484. © 2016 AlphaMed Press.

A smart monitoring infrastructure design for distributed renewable energy systems

International Nuclear Information System (INIS)

Kabalci, Ersan

2015-01-01

Highlights: • A smart grid model for renewable energy sources is proposed in this study. • The renewable energy conversion system is constituted with solar plants and full bridge inverter. • The power line communication is performed with QPSK modulation. • The transmission line that has 25 km length is modelled with real-time parameters. • The efficiency of system is analysed by comparing transmitted and received data. - Abstract: The automatic meter reading is essentially required in renewable grids as in conventional grids. It is intended to propose a reliable measurement system that is validated in a photovoltaic power system to meet the requirement of a renewable grid. In the presented study, the photovoltaic plants are controlled by using a widely known maximum power point tracking algorithm that is named as “Perturb and Observe”. The distribution line at the output of inverter is modelled according to realistic parameters of 25 km line. Besides carrying the generated line voltage, the grid is used as a transmission medium for the generated power measurements of photovoltaic plants and power consumptions of load plants separately. The modem constituting the power line communication manages the dual-channel transfer and transmits the consumed energy ratios of the load plants. One of the modems is located at the output of voltage source inverter and the other one of the load plants. The power consumption values of each load plants are individually measured and successfully transmitted to monitoring section in the modelled system. The obtained data that is only used for monitoring in this application can also be evaluated for automatic meter reading applications

Economic Justification of Concentrating Solar Power in High Renewable Energy Penetrated Power Systems

Energy Technology Data Exchange (ETDEWEB)

Hodge, Brian S [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Kroposki, Benjamin D [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Du, Ershun [Tsinghua University; Zhang, Ning [Tsinghua University; Kang, Chongqing [Tsinghua University; Xia, Qing [Tsinghua University

2018-04-24

Concentrating solar power (CSP) plants are able to provide both renewable energy and operational flexibility at the same time due to its thermal energy storage (TES). It is ideal generation to power systems lacking in flexibility to accommodate variable renewable energy (VRE) generation such as wind power and photovoltaics. However, its investment cost currently is too high to justify its benefit in terms of providing renewable energy only. In this paper we evaluate the economic benefit of CSP in high renewable energy penetrated power systems from two aspects: generating renewable energy and providing operational flexibility to help accommodating VRE. In order to keep the same renewable energy penetration level during evaluation, we compare the economic costs between the system with a high share of VRE and another in which some part of the VRE generation is replaced by CSP generation. The generation cost of a power system is analyzed through chronological operation simulation over a whole year. The benefit of CSP is quantified into two parts: (1) energy benefit - the saving investment of substituted VRE generation and (2) flexibility benefit - the reduction in operating cost due to substituting VRE with CSP. The break-even investment cost of CSP is further discussed. The methodology is tested on a modified IEEE RTS-79 system. The economic justifications of CSP are demonstrated in two practical provincial power systems with high penetration of renewable energy in northwestern China, Qinghai and Gansu, where the former province has massive inflexible thermal power plants but later one has high share of flexible hydro power. The results suggest that the CSP is more beneficial in Gansu system than in Qinghai. The levelized benefit of CSP, including both energy benefit and flexibility benefit, is about 0.177-0.191 $/kWh in Qinghai and about 0.238-0.300 $/kWh in Gansu, when replacing 5-20% VRE generation with CSP generation.

Renewable energy for sustainable electrical energy system in India

International Nuclear Information System (INIS)

Mallah, Subhash; Bansal, N.K.

2010-01-01

Present trends of electrical energy supply and demand are not sustainable because of the huge gap between demand and supply in foreseeable future in India. The path towards sustainability is exploitation of energy conservation and aggressive use of renewable energy systems. Potential of renewable energy technologies that can be effectively harnessed would depend on future technology developments and breakthrough in cost reduction. This requires adequate policy guidelines and interventions in the Indian power sector. Detailed MARKAL simulations, for power sector in India, show that full exploitation of energy conservation potential and an aggressive implementation of renewable energy technologies lead to sustainable development. Coal and other fossil fuel (gas and oil) allocations stagnated after the year 2015 and remain constant up to 2040. After the year 2040, the requirement for coal and gas goes down and carbon emissions decrease steeply. By the year 2045, 25% electrical energy can be supplied by renewable energy and the CO 2 emissions can be reduced by 72% as compared to the base case scenario. (author)

Stability Enhancement of a Power System Containing High-Penetration Intermittent Renewable Generation

Directory of Open Access Journals (Sweden)

Jorge Morel

2015-06-01

Full Text Available This paper considers the transient stability enhancement of a power system containing large amounts of solar and wind generation in Japan. Following the Fukushima Daiichi nuclear disaster there has been an increasing awareness on the importance of a distributed architecture, based mainly on renewable generation, for the Japanese power system. Also, the targets of CO2 emissions can now be approached without heavily depending on nuclear generation. Large amounts of renewable generation leads to a reduction in the total inertia of the system because renewable generators are connected to the grid by power converters, and transient stability becomes a significant issue. Simulation results show that sodium-sulfur batteries can keep the system in operation and stable after strong transient disturbances, especially for an isolated system. The results also show how the reduction of the inertia in the system can be mitigated by exploiting the kinetic energy of wind turbines.

CXCR6, a newly defined biomarker of tissue-specific stem cell asymmetric self-renewal, identifies more aggressive human melanoma cancer stem cells.

Directory of Open Access Journals (Sweden)

Rouzbeh Taghizadeh

2010-12-01

Full Text Available A fundamental problem in cancer research is identifying the cell type that is capable of sustaining neoplastic growth and its origin from normal tissue cells. Recent investigations of a variety of tumor types have shown that phenotypically identifiable and isolable subfractions of cells possess the tumor-forming ability. In the present paper, using two lineage-related human melanoma cell lines, primary melanoma line IGR39 and its metastatic derivative line IGR37, two main observations are reported. The first one is the first phenotypic evidence to support the origin of melanoma cancer stem cells (CSCs from mutated tissue-specific stem cells; and the second one is the identification of a more aggressive subpopulation of CSCs in melanoma that are CXCR6+.We defined CXCR6 as a new biomarker for tissue-specific stem cell asymmetric self-renewal. Thus, the relationship between melanoma formation and ABCG2 and CXCR6 expression was investigated. Consistent with their non-metastatic character, unsorted IGR39 cells formed significantly smaller tumors than unsorted IGR37 cells. In addition, ABCG2+ cells produced tumors that had a 2-fold greater mass than tumors produced by unsorted cells or ABCG2- cells. CXCR6+ cells produced more aggressive tumors. CXCR6 identifies a more discrete subpopulation of cultured human melanoma cells with a more aggressive MCSC phenotype than cells selected on the basis of the ABCG2+ phenotype alone.The association of a more aggressive tumor phenotype with asymmetric self-renewal phenotype reveals a previously unrecognized aspect of tumor cell physiology. Namely, the retention of some tissue-specific stem cell attributes, like the ability to asymmetrically self-renew, impacts the natural history of human tumor development. Knowledge of this new aspect of tumor development and progression may provide new targets for cancer prevention and treatment.

Life cycle emissions from renewable energy technologies

International Nuclear Information System (INIS)

Bates, J.; Watkiss, P.; Thorpe, T.

1997-01-01

This paper presents the methodology used in the ETSU review, together with the detailed results for three of the technologies studied: wind turbines, photovoltaic systems and small, stand-alone solar thermal systems. These emissions are then compared with those calculated for both other renewables and fossil fuel technology on a similar life cycle basis. The life cycle emissions associated with renewable energy technology vary considerably. They are lowest for those technologies where the renewable resource has been concentrated in some way (e.g. over distance in the case of wind and hydro, or over time in the case of energy crops). Wind turbines have amongst the lowest emissions of all renewables and are lower than those for fossil fuel generation, often by over an order of magnitude. Photovoltaics and solar thermal systems have the highest life cycle emissions of all the renewable energy technologies under review. However, their emissions of most pollutants are also much lower than those associated with fossil fuel technologies. In addition, the emissions associated with PV are likely to fall further in the future as the conversion efficiency of PV cells increases and manufacturing technology switches to thin film technologies, which are less energy intensive. Combining the assessments of life cycle emissions of renewables with predictions made by the World Energy Council (WEC) of their future deployment has allowed estimates to be made of amount by which renewables could reduce the future global emissions of carbon dioxide, sulphur dioxide and nitrogen oxides. It estimated that under the WEC's 'Ecologically Driven' scenario, renewables might lead to significant reductions of between 3650 and 8375 Mt in annual CO 2 emissions depending on the fossil fuel technology they are assumed to displace. (author)

The Evolution of Power System Planning with High Levels of Variable Renewable Generation

Energy Technology Data Exchange (ETDEWEB)

Katz, Jessica [National Renewable Energy Lab. (NREL), Golden, CO (United States); Milligan, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States)

2016-09-01

Greening the Grid provides technical assistance to energy system planners, regulators, and grid operators to overcome challenges associated with integrating variable renewable energy into the grid. This document, part of the Greening the Grid introduces the evolution of power system planning with high levels of variable renewable generation.

Stability of impulsive systems driven by renewal processes

NARCIS (Netherlands)

Guerreiro Tome Antunes, D.J.; Hespanha, J.P.; Silvestre, C.J.

2009-01-01

Necessary and sufficient conditions are provided for stochastic stability and mean exponential stability of impulsive systems with jumps triggered by a renewal process, that is, the intervals between jumps are independent and identically distributed. The conditions for stochastic stability can be

Optimum transmission system expansion offshore considering renewable energy sources

NARCIS (Netherlands)

Torbaghan, Shahab S.; Gibescu, Madeleine

2017-01-01

This chapter provides an overview of transmission expansion planning (TEP) methods and their practical application. First, it discusses the strategic importance of the transmission system. Next, it describes the reasons why TEP remains a challenge for systems with a large share of renewable energy.

SVM classifier to predict genes important for self-renewal and pluripotency of mouse embryonic stem cells

Directory of Open Access Journals (Sweden)

Xu Huilei

2010-12-01

Full Text Available Abstract Background Mouse embryonic stem cells (mESCs are derived from the inner cell mass of a developing blastocyst and can be cultured indefinitely in-vitro. Their distinct features are their ability to self-renew and to differentiate to all adult cell types. Genes that maintain mESCs self-renewal and pluripotency identity are of interest to stem cell biologists. Although significant steps have been made toward the identification and characterization of such genes, the list is still incomplete and controversial. For example, the overlap among candidate self-renewal and pluripotency genes across different RNAi screens is surprisingly small. Meanwhile, machine learning approaches have been used to analyze multi-dimensional experimental data and integrate results from many studies, yet they have not been applied to specifically tackle the task of predicting and classifying self-renewal and pluripotency gene membership. Results For this study we developed a classifier, a supervised machine learning framework for predicting self-renewal and pluripotency mESCs stemness membership genes (MSMG using support vector machines (SVM. The data used to train the classifier was derived from mESCs-related studies using mRNA microarrays, measuring gene expression in various stages of early differentiation, as well as ChIP-seq studies applied to mESCs profiling genome-wide binding of key transcription factors, such as Nanog, Oct4, and Sox2, to the regulatory regions of other genes. Comparison to other classification methods using the leave-one-out cross-validation method was employed to evaluate the accuracy and generality of the classification. Finally, two sets of candidate genes from genome-wide RNA interference screens are used to test the generality and potential application of the classifier. Conclusions Our results reveal that an SVM approach can be useful for prioritizing genes for functional validation experiments and complement the analyses of high

Comprehensive analysis of miRNAs expression profiles revealed potential key miRNA/mRNAs regulating colorectal cancer stem cell self-renewal.

Science.gov (United States)

Xu, Peng; Wang, Junhua; Sun, Bo; Xiao, Zhongdang

2018-05-20

Self-renewal is essential for the malignant biological behaviors of colorectal cancer stem cells. While the self-renewal molecular mechanisms of colorectal cancer stem cells are not yet fully understood. Recently, miRNAs are reported to be relevant to the self-renewal ability of cancer stem cells. In this study, we first isolated colorectal cancer stem cell from colorectal cancer cell line HCT-116 by 1% low serum culture. Then we conducted a comprehensive analysis based on the miRNAs profiles data of both colorectal cancer stem cells and normal cultured colorectal cancer cells. Pathway analysis revealed multiple pathways including Jak-STAT, TGF-beta, PI3K-Akt and MAPK signaling pathway that are correlated to colorectal cancer. Further, we constructed a miRNA-mRNA network, based on which, several miRNA/mRNA pairs were ranked according to their impact index to the self-renewal of colorectal cancer stem cells. Further biological experiment showed that up-regulation of miR-92a-3p led to cell cycle arrest and reduced colony formation. This work provides clues to find the new potential biomarkers for colorectal cancer stem cell diagnosis and select effective miRNAs for targeted therapy. Copyright © 2018 Elsevier B.V. All rights reserved.

Design and Realization of Online Monitoring System of Distributed New Energy and Renewable Energy

Science.gov (United States)

Tang, Yanfen; Zhou, Tao; Li, Mengwen; Zheng, Guotai; Li, Hao

2018-01-01

Aimed at difficult centralized monitoring and management of current distributed new energy and renewable energy generation projects due to great varieties, different communication protocols and large-scale difference, this paper designs a online monitoring system of new energy and renewable energy characterized by distributed deployment, tailorable functions, extendible applications and fault self-healing performance. This system is designed based on international general standard for grid information data model, formulates unified data acquisition and transmission standard for different types of new energy and renewable energy generation projects, and can realize unified data acquisition and real-time monitoring of new energy and renewable energy generation projects, such as solar energy, wind power, biomass energy, etc. within its jurisdiction. This system has applied in Beijing. At present, 576 projects are connected to the system. Good effect is achieved and stability and reliability of the system have been validated.

Power control based on particle swarm optimization of grid-connected inverter for hybrid renewable energy system

International Nuclear Information System (INIS)

García-Triviño, Pablo; Gil-Mena, Antonio José; Llorens-Iborra, Francisco; García-Vázquez, Carlos Andrés; Fernández-Ramírez, Luis M.; Jurado, Francisco

2015-01-01

Highlights: • Three PSO-based PI controllers for a grid-connected inverter were presented. • Two online PSO-based PI controllers were compared with an offline PSO-tuned PI. • The HRES and the inverter were evaluated under power changes and grid voltage sags. • Online ITAE-based PSO reduced ITAE (current THD) by 15.24% (5.32%) versus offline one. - Abstract: This paper is focused on the study of particle swarm optimization (PSO)-based PI controllers for the power control of a grid-connected inverter supplied from a hybrid renewable energy system. It is composed of two renewable energy sources (wind turbine and photovoltaic – PV – solar panels) and two energy storage systems (battery and hydrogen system, integrated by fuel cell and electrolyzer). Three PSO-based PI controllers are implemented: (1) conventional PI controller with offline tuning by PSO algorithm based on the integral time absolute error (ITAE) index; (2) PI controllers with online self-tuning by PSO algorithm based on the error; and (3) PI controllers with online self-tuning by PSO algorithm based on the ITAE index. To evaluate and compare the three controllers, the hybrid renewable energy system and the grid-connected inverter are simulated under changes in the active and reactive power values, as well as under a grid voltage sag. The results show that the online PSO-based PI controllers that optimize the ITAE index achieves the best response

Renewal processes

CERN Document Server

Mitov, Kosto V

2014-01-01

This monograph serves as an introductory text to classical renewal theory and some of its applications for graduate students and researchers in mathematics and probability theory. Renewal processes play an important part in modeling many phenomena in insurance, finance, queuing systems, inventory control and other areas. In this book, an overview of univariate renewal theory is given and renewal processes in the non-lattice and lattice case are discussed. A pre-requisite is a basic knowledge of probability theory.

Integrated transport and renewable energy systems

DEFF Research Database (Denmark)

Mathiesen, Brian Vad; Lund, Henrik; Nørgaard, P.

2008-01-01

No single technology can solve the problem of ever increasing CO2 emissions from transport. Here, a coherent effort to integrate transport into energyplanning is proposed, using multiple means promoting sustainable transport. It is concluded that a 100 per cent renewable energy transport system...... is possible but is connected to significant challenges in the path towards it. Biomass is a limited resource and it is important to avoid effecting the production of food. The integration of the transport with the energy system is crucial as is a multi-pronged strategy. Short term solutions have to consider...

Role of renewable energy policies in energy dependency in Finland: System dynamics approach

International Nuclear Information System (INIS)

Aslani, Alireza; Helo, Petri; Naaranoja, Marja

2014-01-01

Highlights: • A system dynamics model for evaluating renewable energy policies on dependency is proposed. • The model considers the role of diversification on dependency and security of energy supply in Finland. • Dependency on imported sources will decrease depends on the defined scenarios in Finland. - Abstract: Objective: We discuss the role of diversification on dependency and security of energy supply. A system dynamics model with especial focus on the role of renewable energy resources (as a portfolio) on Finland’s energy dependency is developed. The purpose is also to cover a part of research gap exists in the system dynamics modeling of energy security investigations. Methods: A causal loops diagram and a system dynamics model evaluate Finnish scenarios of renewable energy policies. The analysis describes the relationship between dynamic factors such as RE encouragement packages, dependency, and energy demand. Results: A causal loops diagram and a system dynamics model evaluate three different Finnish scenarios of renewable energy policies by 2020. Conclusion: Analysis shows that despite 7% electricity/heat consumption growth by 2020 in Finland, dependency on imported sources will decrease between 1% and 7% depend on the defined scenarios. Practice Implications: The proposed model not only helps decision makers to test their scenarios related to renewable energy polices, it can be implemented by other countries

Integrated operation of electric vehicles and renewable generation in a smart distribution system

International Nuclear Information System (INIS)

Zakariazadeh, Alireza; Jadid, Shahram; Siano, Pierluigi

2015-01-01

Highlights: • The contribution of electric vehicles to provide the reserve capacity is analyzed. • Decentralized energy and reserve scheduling in a distribution system is presented. • The integrated operation of renewable generation and electric vehicles is proposed. - Abstract: Distribution system complexity is increasing mainly due to technological innovation, renewable Distributed Generation (DG) and responsive loads. This complexity makes difficult the monitoring, control and operation of distribution networks for Distribution System Operators (DSOs). In order to cope with this complexity, a novel method for the integrated operational planning of a distribution system is presented in this paper. The method introduces the figure of the aggregator, conceived as an intermediate agent between end-users and DSOs. In the proposed method, energy and reserve scheduling is carried out by both aggregators and DSO. Moreover, Electric Vehicles (EVs) are considered as responsive loads that can participate in ancillary service programs by providing reserve to the system. The efficiency of the proposed method is evaluated on an 84-bus distribution test system. Simulation results show that the integrated scheduling of EVs and renewable generators can mitigate the negative effects related to the uncertainty of renewable generation

The sonic hedgehog signaling pathway maintains the cancer stem cell self-renewal of anaplastic thyroid cancer by inducing snail expression.

Science.gov (United States)

Heiden, Katherine B; Williamson, Ashley J; Doscas, Michelle E; Ye, Jin; Wang, Yimin; Liu, Dingxie; Xing, Mingzhao; Prinz, Richard A; Xu, Xiulong

2014-11-01

Cancer stem cells (CSCs) have been recently identified in thyroid neoplasm. Anaplastic thyroid cancer (ATC) contains a higher percentage of CSCs than well-differentiated thyroid cancer. The signaling pathways and the transcription factors that regulate thyroid CSC self-renewal remain poorly understood. The objective of this study is to use two ATC cell lines (KAT-18 and SW1736) as a model to study the role of the sonic hedgehog (Shh) pathway in maintaining thyroid CSC self-renewal and to understand its underlying molecular mechanisms. The expression and activity of aldehyde dehydrogenase (ALDH), a marker for thyroid CSCs, was analyzed by Western blot and ALDEFLUOR assay, respectively. The effect of three Shh pathway inhibitors (cyclopamine, HhAntag, GANT61), Shh, Gli1, Snail knockdown, and Gli1 overexpression on thyroid CSC self-renewal was analyzed by ALDEFLUOR assay and thyrosphere formation. The sensitivity of transfected KAT-18 cells to radiation was evaluated by a colony survival assay. Western blot analysis revealed that ALDH protein levels in five thyroid cancer cell lines (WRO82, a follicular thyroid cancer cell line; BCPAP and TPC1, two papillary thyroid cancer cell lines; KAT-18 and SW1736, two ATC cell lines) correlated with the percentage of the ALDH(High) cells as well as Gli1 and Snail expression. The Shh pathway inhibitors, Shh and Gli1 knockdown, in KAT-18 cells decreased thyroid CSC self-renewal and increased radiation sensitivity. In contrast, Gli1 overexpression led to increased thyrosphere formation, an increased percentage of ALDH(High) cells, and increased radiation resistance in KAT-18 cells. Inhibition of the Shh pathway by three specific inhibitors led to decreased Snail expression and a decreased number of ALDH(High) cells in KAT-18 and SW1736. Snail gene knockdown decreased the number of ALDH(High) cells in KAT-18 and SW1736 cells. The Shh pathway promotes the CSC self-renewal in ATC cell lines by Gli1-induced Snail expression.

Integrated transcriptome and binding sites analysis implicates E2F in the regulation of self-renewal in human pluripotent stem cells.

Directory of Open Access Journals (Sweden)

Hock Chuan Yeo

Full Text Available Rapid cellular growth and multiplication, limited replicative senescence, calibrated sensitivity to apoptosis, and a capacity to differentiate into almost any cell type are major properties that underline the self-renewal capabilities of human pluripotent stem cells (hPSCs. We developed an integrated bioinformatics pipeline to understand the gene regulation and functions involved in maintaining such self-renewal properties of hPSCs compared to matched fibroblasts. An initial genome-wide screening of transcription factor activity using in silico binding-site and gene expression microarray data newly identified E2F as one of major candidate factors, revealing their significant regulation of the transcriptome. This is underscored by an elevated level of its transcription factor activity and expression in all tested pluripotent stem cell lines. Subsequent analysis of functional gene groups demonstrated the importance of the TFs to self-renewal in the pluripotency-coupled context; E2F directly targets the global signaling (e.g. self-renewal associated WNT and FGF pathways and metabolic network (e.g. energy generation pathways, molecular transports and fatty acid metabolism to promote its canonical functions that are driving the self-renewal of hPSCs. In addition, we proposed a core self-renewal module of regulatory interplay between E2F and, WNT and FGF pathways in these cells. Thus, we conclude that E2F plays a significant role in influencing the self-renewal capabilities of hPSCs.

IEA-R1 renewed primary coolant piping system stress analysis

International Nuclear Information System (INIS)

Fainer, Gerson; Faloppa, Altair A.; Oliveira, Carlos A. de; Mattar Neto, Miguel

2015-01-01

A partial replacement of the IEA-R1 piping system was conducted in 2014. The aim of this work is to perform the stress analysis of the renewed primary piping system of the IEA-R1, taking into account the as built conditions and the pipe modifications. The nuclear research reactor IEA-R1 is a pool type reactor designed by Babcox-Willcox, which is operated by IPEN since 1957. The primary coolant system is responsible for removing the residual heat of the Reactor core. As a part of the life management, a regular inspection detected some degradation in the primary piping system. In consequence, part of the piping system was replaced. The partial renewing of the primary piping system did not imply in major piping layout modifications. However, the stress condition of the piping systems had to be reanalyzed. The structural stress analysis of the primary piping systems is now presented and the final results are discussed. (author)

Expert Opinion Analysis on Renewable Hydrogen Storage Systems Potential in Europe

Directory of Open Access Journals (Sweden)

Davide Astiaso Garcia

2016-11-01

Full Text Available Among the several typologies of storage technologies, mainly on different physical principles (mechanical, electrical and chemical, hydrogen produced by power to gas (P2G from renewable energy sources complies with chemical storage principle and is based on the conversion of electrical energy into chemical energy by means of the electrolysis of water which does not produce any toxic or climate-relevant emission. This paper aims to pinpoint the potential uses of renewable hydrogen storage systems in Europe, analysing current and potential locations, regulatory framework, governments’ outlooks, economic issues, and available renewable energy amounts. The expert opinion survey, already used in many research articles on different topics including energy, has been selected as an effective method to produce realistic results. The obtained results highlight strategies and actions to optimize the storage of hydrogen produced by renewables to face varying electricity demand and generation-driven fluctuations reducing the negative effects of the increasing share of renewables in the energy mix of European Countries.

Nuclear Energy and Renewables: System Effects in Low-carbon Electricity Systems : Method comments to a NEA report

OpenAIRE

Söder, Lennart

2012-01-01

OECD Nuclear Energy Agency (NEA) released a new report on 29 November 2012. The study recommends that decision-makers should take full electricity system costs into account in energy choices and that such costs should be internalised according to a “generator pays” principle. The study, entitled Nuclear Energy and Renewables: System Effects in Low-carbon Electricity Systems, addresses the increasingly important interactions of variable renewables and dispatchable energy technologies, such as ...

GIS-based site selection methodology for hybrid renewable energy systems: A case study from western Turkey

International Nuclear Information System (INIS)

Aydin, Nazli Yonca; Kentel, Elcin; Sebnem Duzgun, H.

2013-01-01

Highlights: ► We proposed a site selection methodology for renewable energy systems. ► Fuzzy logic and geographic information system tools are used. ► Alternative locations are evaluated in terms of economic and environmental criteria. - Abstract: Renewable energy sources are presently being considered as alternatives to fossil fuels, because they are perpetual, environmentally friendly, and release negligible amounts of greenhouse gases to the atmosphere while producing energy. A disadvantage of renewable energy systems, however, is that continuous energy generation is not possible by using only one type of renewable energy system, since renewable energy resources depend on climate and weather conditions. Two or more renewable energy systems can be integrated into a hybrid system to overcome this problem so that when one resource is not available, the other can continue producing energy. Another disadvantage of renewable energy sources is that they are not available at every geographic location. Their use is mostly advantageous at remote locations that often are of high ecological value. Thus, identification of preferable locations for renewable energy systems is a decision-making problem that requires evaluation of the potential of the resource together with economic and environmental limitations. This paper introduces a methodology for site selection of hybrid wind solar–PV renewable energy systems. First, environmental acceptability and economic feasibility objectives are identified through a comprehensive review of the literature, current Turkish laws and legislations, and interviews with the General Directorate of Electrical Power Resources Survey and Development Administration of Turkey. Second, viable locations in terms of environmental acceptability and economic feasibility are determined through a fuzzy decision-making procedure that uses ordered weighted averaging algorithm for aggregating multiple objectives. Then, priority sites are identified

Renewable Electricity-to-Grid Integration | Energy Systems Integration

Science.gov (United States)

Facility | NREL Renewable Electricity-to-Grid Integration Renewable Electricity-to-Grid Integration NREL works with industry partners to optimize strategies for effectively interconnecting renewable renewable electric grid integration work includes research and development (R&D) on advanced inverters

Feasibility study and energy conversion analysis of stand-alone hybrid renewable energy system

International Nuclear Information System (INIS)

Baghdadi, Fazia; Mohammedi, Kamal; Diaf, Said; Behar, Omar

2015-01-01

Highlights: • Hybrid stand-alone wind–solar–fossil power system is analyzed. • Measurement data are used to evaluate system performance. • The proposed system can generate about 70% from renewables. • Such a hybrid plant is very promising for remote regions in Algeria. - Abstract: There is a great interest in the development of renewable power technologies in Algeria, and more particularly hybrid concept. The present paper has investigated the performance of hybrid PV–Wind–Diesel–Battery configuration based on hourly measurements of Adrar climate (southern Algeria). Data of global solar radiation, ambient temperature and wind speed for a period of one year have been used. Firstly, the proposed hybrid system has been optimized by means of HOMER software. The optimization process has been carried out taking into account renewable resources potential and energy demand; while maximizing renewable electricity use and fuel saving are the purpose. In the second step, a mathematical model has been developed to ensure efficient energy management on the basis of various operation strategies. The analysis has shown that renewable energy system (PV–Wind) is able to supply about 70% of the demand. Wind power has ranked first with 43% of the annual total electricity production followed by diesel generator (with 31%) while the remaining fraction is being to PV panels. In this context, 69% of the fossil fuel can be saved when using the proposed hybrid configuration instead of the diesel generators that are currently installed in most remote regions in Algeria. Such a concept is very promising to meet the focus of renewable energy program announced in 2011.

Roles of Retinoids and Retinoic Acid Receptors in the Regulation of Hematopoietic Stem Cell Self-Renewal and Differentiation

Directory of Open Access Journals (Sweden)

Louise E. Purton

2007-01-01

Full Text Available Multipotent hematopoietic stem cells (HSCs sustain blood cell production throughout an individual's lifespan through complex processes ultimately leading to fates of self-renewal, differentiation or cell death decisions. A fine balance between these decisions in vivo allows for the size of the HSC pool to be maintained. While many key factors involved in regulating HSC/progenitor cell differentiation and cell death are known, the critical regulators of HSC self-renewal are largely unknown. In recent years, however, a number of studies describing methods of increasing or decreasing the numbers of HSCs in a given population have emerged. Of major interest here are the emerging roles of retinoids in the regulation of HSCs.

Endogenous production of fibronectin is required for self-renewal of cultured mouse embryonic stem cells

OpenAIRE

Hunt, Geoffrey C.; Singh, Purva; Schwarzbauer, Jean E.

2012-01-01

Pluripotent cells are attached to the extracellular matrix (ECM) as they make cell fate decisions within the stem cell niche. Here we show that the ubiquitous ECM protein fibronectin is required for self-renewal decisions by cultured mouse embryonic stem (mES) cells. Undifferentiated mES cells produce fibronectin and assemble a fibrillar matrix. Increasing the level of substrate fibronectin increased cell spreading and integrin receptor signaling through focal adhesion kinase, while concomita...

Self-renewal of CD133(hi) cells by IL6/Notch3 signalling regulates endocrine resistance in metastatic breast cancer.

Science.gov (United States)

Sansone, Pasquale; Ceccarelli, Claudio; Berishaj, Marjan; Chang, Qing; Rajasekhar, Vinagolu K; Perna, Fabiana; Bowman, Robert L; Vidone, Michele; Daly, Laura; Nnoli, Jennifer; Santini, Donatella; Taffurelli, Mario; Shih, Natalie N C; Feldman, Michael; Mao, Jun J; Colameco, Christopher; Chen, Jinbo; DeMichele, Angela; Fabbri, Nicola; Healey, John H; Cricca, Monica; Gasparre, Giuseppe; Lyden, David; Bonafé, Massimiliano; Bromberg, Jacqueline

2016-02-09

The mechanisms of metastatic progression from hormonal therapy (HT) are largely unknown in luminal breast cancer. Here we demonstrate the enrichment of CD133(hi)/ER(lo) cancer cells in clinical specimens following neoadjuvant endocrine therapy and in HT refractory metastatic disease. We develop experimental models of metastatic luminal breast cancer and demonstrate that HT can promote the generation of HT-resistant, self-renewing CD133(hi)/ER(lo)/IL6(hi) cancer stem cells (CSCs). HT initially abrogates oxidative phosphorylation (OXPHOS) generating self-renewal-deficient cancer cells, CD133(hi)/ER(lo)/OXPHOS(lo). These cells exit metabolic dormancy via an IL6-driven feed-forward ER(lo)-IL6(hi)-Notch(hi) loop, activating OXPHOS, in the absence of ER activity. The inhibition of IL6R/IL6-Notch pathways switches the self-renewal of CD133(hi) CSCs, from an IL6/Notch-dependent one to an ER-dependent one, through the re-expression of ER. Thus, HT induces an OXPHOS metabolic editing of luminal breast cancers, paradoxically establishing HT-driven self-renewal of dormant CD133(hi)/ER(lo) cells mediating metastatic progression, which is sensitive to dual targeted therapy.

The HPV16 E7 oncoprotein increases the expression of Oct3/4 and stemness-related genes and augments cell self-renewal

Energy Technology Data Exchange (ETDEWEB)

Organista-Nava, Jorge; Gómez-Gómez, Yazmín [Programa de Doctorado en Ciencias Biomédicas, Instituto de Fisiología Celular (IFC), Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, México (Mexico); Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México 07360, México (Mexico); Ocadiz-Delgado, Rodolfo; García-Villa, Enrique [Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México 07360, México (Mexico); Bonilla-Delgado, José [Unidad de Investigación, Hospital Juárez de México, Ciudad de México 07760, México (Mexico); Lagunas-Martínez, Alfredo [División de Biología Molecular de Patógenos, CISEI, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México (Mexico); and others

2016-12-15

Oct3/4 is a transcription factor involved in maintenance of the pluripotency and self-renewal of stem cells. The E7 oncoprotein and 17β-estradiol (E{sub 2}) are key factors in cervical carcinogenesis. In the present study, we aimed to investigate the effect of the HPV16 E7 oncoprotein and E{sub 2} on the expression pattern of Oct3/4, Sox2, Nanog and Fgf4. We also determined whether the E7 oncoprotein is associated with cell self-renewal. The results showed that Oct3/4, Sox2, Nanog and Fgf4 were upregulated by the E7 oncoprotein in vivo and in vitro and implicate E{sub 2} in the upregulation of these factors in vivo. We also demonstrated that E7 is involved in cell self-renewal, suggesting that the HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal capacity of cancer stem cells. -- Graphical abstract: The HPV16 E7 oncoprotein and 17β-estradiol are involved in the upregulation of Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal ability of cancer stem cells in cervical cancer. - Highlights: •The HPV16 E7 oncoprotein enhances cellular proliferation and dedifferentiation. •The E7 oncoprotein induces stemness-related genes expression in vivo and in vitro. •The 17β-estradiol induces stemness-related genes expression in vivo. •The HPV16 E7 oncoprotein is involved in the cell self-renewal of cancer cells.

The HPV16 E7 oncoprotein increases the expression of Oct3/4 and stemness-related genes and augments cell self-renewal

International Nuclear Information System (INIS)

Organista-Nava, Jorge; Gómez-Gómez, Yazmín; Ocadiz-Delgado, Rodolfo; García-Villa, Enrique; Bonilla-Delgado, José; Lagunas-Martínez, Alfredo

2016-01-01

Oct3/4 is a transcription factor involved in maintenance of the pluripotency and self-renewal of stem cells. The E7 oncoprotein and 17β-estradiol (E 2 ) are key factors in cervical carcinogenesis. In the present study, we aimed to investigate the effect of the HPV16 E7 oncoprotein and E 2 on the expression pattern of Oct3/4, Sox2, Nanog and Fgf4. We also determined whether the E7 oncoprotein is associated with cell self-renewal. The results showed that Oct3/4, Sox2, Nanog and Fgf4 were upregulated by the E7 oncoprotein in vivo and in vitro and implicate E 2 in the upregulation of these factors in vivo. We also demonstrated that E7 is involved in cell self-renewal, suggesting that the HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal capacity of cancer stem cells. -- Graphical abstract: The HPV16 E7 oncoprotein and 17β-estradiol are involved in the upregulation of Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal ability of cancer stem cells in cervical cancer. - Highlights: •The HPV16 E7 oncoprotein enhances cellular proliferation and dedifferentiation. •The E7 oncoprotein induces stemness-related genes expression in vivo and in vitro. •The 17β-estradiol induces stemness-related genes expression in vivo. •The HPV16 E7 oncoprotein is involved in the cell self-renewal of cancer cells.

Development and commercialization of renewable energy technologies in Canada: An innovation system perspective

International Nuclear Information System (INIS)

Jagoda, Kalinga; Lonseth, Robert; Lonseth, Adam; Jackman, Tom

2011-01-01

The increased environmental awareness coupled with the recent changes in the oil prices triggered the necessity of focusing on effective management of energy systems. Global climate change has caused many people to consider ways of reducing greenhouse gases Renewable energy has become an essential feature in curtailing emission of Green House Gases, while meeting the demand for energy. This paper presents an innovation system framework for development and diffusion of renewable energy technologies. The framework is used to identify opportunities for small and medium enterprises in the renewable energy sector. A case study on a successful development, installation and implementation of solar thermal systems households in Calgary, Alberta, by an entrepreneurial firm, is also presented. (author)

Development and commercialization of renewable energy technologies in Canada: An innovation system perspective

Energy Technology Data Exchange (ETDEWEB)

Jagoda, Kalinga; Lonseth, Robert; Lonseth, Adam [Bissett School of Business, Mount Royal University, 4825 Mount Royal Gate SW, Calgary AB T3E 6K6 (Canada); Jackman, Tom [Simple Solar Heating Limited, P.O. Box 988, Okotoks AB T1S 1B1 (Canada)

2011-04-15

The increased environmental awareness coupled with the recent changes in the oil prices triggered the necessity of focusing on effective management of energy systems. Global climate change has caused many people to consider ways of reducing greenhouse gases Renewable energy has become an essential feature in curtailing emission of Green House Gases, while meeting the demand for energy. This paper presents an innovation system framework for development and diffusion of renewable energy technologies. The framework is used to identify opportunities for small and medium enterprises in the renewable energy sector. A case study on a successful development, installation and implementation of solar thermal systems households in Calgary, Alberta, by an entrepreneurial firm, is also presented. (author)

Review of the Optimal Design on a Hybrid Renewable Energy System

Directory of Open Access Journals (Sweden)

Wu Yuan-Kang

2016-01-01

Full Text Available Hybrid renewable energy systems, combining various kinds of technologies, have shown relatively high capabilities to solve reliability problems and have reduced cost challenges. The use of hybrid electricity generation/storage technologies is reasonable to overcome related shortcomings. While the hybrid renewable energy system is attractive, its design, specifically the determination of the size of PV, wind, and diesel power generators and the size of energy storage system in each power station, is very challenging. Therefore, this paper will focus on the system planning and operation of hybrid generation systems, and several corresponding topics and papers by using intelligent computing methods will be reviewed. They include typical case studies, modeling and system simulation, control and management, reliability and economic studies, and optimal design on a reliable hybrid generation system.

Neutral dynamics and cell renewal of colonic crypts in homeostatic regime

Science.gov (United States)

Fendrik, A. J.; Romanelli, L.; Rotondo, E.

2018-05-01

The self renewal process in colonic crypts is the object of several studies. We present here a new compartment model with the following characteristics: (a) we distinguish different classes of cells: stem cells, six generations of transit amplifying cells and the differentiated cells; (b) in order to take into account the monoclonal character of crypts in homeostatic regimes we include symmetric divisions of the stem cells. We first consider the dynamic differential equations that describe the evolution of the mean values of the populations, but the small observed value of the total number of cells involved plus the huge dispersion of experimental data found in the literature leads us to study the stochastic discrete process. This analysis allows us to study fluctuations, the neutral drift that leads to monoclonality, and the effects of the fixation of mutant clones.

RNA-Binding Protein L1TD1 Interacts with LIN28 via RNA and is Required for Human Embryonic Stem Cell Self-Renewal and Cancer Cell Proliferation

OpenAIRE

Närvä, Elisa; Rahkonen, Nelly; Emani, Maheswara Reddy; Lund, Riikka; Pursiheimo, Huha-Pekka; Nästi, Juuso; Autio, Reija; Rasool, Omid; Denessiouk, Konstantin; Lähdesmäki, Harri; Rao, Anjana; Lahesmaa, Ritta

2012-01-01

Human embryonic stem cells (hESC) have a unique capacity to self-renew and differentiate into all the cell types found in human body. Although the transcriptional regulators of pluripotency are well studied, the role of cytoplasmic regulators is still poorly characterized. Here, we report a new stem cell-specific RNA-binding protein L1TD1 (ECAT11, FLJ10884) required for hESC self-renewal and cancer cell proliferation. Depletion of L1TD1 results in immediate downregulation of OCT4 and NANOG. F...

Transient Stability Assessment of Power Systems With Uncertain Renewable Generation: Preprint

Energy Technology Data Exchange (ETDEWEB)

Villegas Pico, Hugo Nestor [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Aliprantis, Dionysios C. [Purdue University; Lin, Xiaojun [Purdue University

2017-08-09

The transient stability of a power system depends heavily on its operational state at the moment of a fault. In systems where the penetration of renewable generation is significant, the dispatch of the conventional fleet of synchronous generators is uncertain at the time of dynamic security analysis. Hence, the assessment of transient stability requires the solution of a system of nonlinear ordinary differential equations with unknown initial conditions and inputs. To this end, we set forth a computational framework that relies on Taylor polynomials, where variables are associated with the level of renewable generation. This paper describes the details of the method and illustrates its application on a nine-bus test system.

A High-Efficiency Voltage Equalization Scheme for Supercapacitor Energy Storage System in Renewable Generation Applications

Directory of Open Access Journals (Sweden)

Liran Li

2016-06-01

Full Text Available Due to its fast charge and discharge rate, a supercapacitor-based energy storage system is especially suitable for power smoothing in renewable energy generation applications. Voltage equalization is essential for series-connected supercapacitors in an energy storage system, because it supports the system’s sustainability and maximizes the available cell energy. In this paper, we present a high-efficiency voltage equalization scheme for supercapacitor energy storage systems in renewable generation applications. We propose an improved isolated converter topology that uses a multi-winding transformer. An improved push-pull forward circuit is applied on the primary side of the transformer. A coupling inductor is added on the primary side to allow the switches to operate under the zero-voltage switching (ZVS condition, which reduces switching losses. The diodes in the rectifier are replaced with metal-oxide-semiconductor field-effect transistors (MOSFETs to reduce the power dissipation of the secondary side. In order to simplify the control, we designed a controllable rectifying circuit to achieve synchronous rectifying on the secondary side of the transformer. The experimental results verified the effectiveness of the proposed design.

Dclk1+ small intestinal epithelial tuft cells display the hallmarks of quiescence and self-renewal

Science.gov (United States)

Chandrakesan, Parthasarathy; May, Randal; Qu, Dongfeng; Weygant, Nathaniel; Taylor, Vivian E.; Li, James D.; Ali, Naushad; Sureban, Sripathi M.; Qante, Michael; Wang, Timothy C.; Bronze, Michael S.; Houchen, Courtney W.

2015-01-01

To date, no discrete genetic signature has been defined for isolated Dclk1+ tuft cells within the small intestine. Furthermore, recent reports on the functional significance of Dclk1+ cells in the small intestine have been inconsistent. These cells have been proposed to be fully differentiated cells, reserve stem cells, and tumor stem cells. In order to elucidate the potential function of Dclk1+ cells, we FACS-sorted Dclk1+ cells from mouse small intestinal epithelium using transgenic mice expressing YFP under the control of the Dclk1 promoter (Dclk1-CreER;Rosa26-YFP). Analysis of sorted YFP+ cells demonstrated marked enrichment (~6000 fold) for Dclk1 mRNA compared with YFP− cells. Dclk1+ population display ~6 fold enrichment for the putative quiescent stem cell marker Bmi1. We observed significantly greater expression of pluripotency genes, pro-survival genes, and quiescence markers in the Dclk1+ population. A significant increase in self-renewal capability (14-fold) was observed in in vitro isolated Dclk1+ cells. The unique genetic report presented in this manuscript suggests that Dclk1+ cells may maintain quiescence, pluripotency, and metabolic activity for survival/longevity. Functionally, these reserve characteristics manifest in vitro, with Dclk1+ cells exhibiting greater ability to self-renew. These findings indicate that quiescent stem-like functionality is a feature of Dclk1-expressing tuft cells. PMID:26362399

Histone deacetylase inhibition enhances self renewal and cardioprotection by human cord blood-derived CD34 cells.

Directory of Open Access Journals (Sweden)

Ilaria Burba

Full Text Available BACKGROUND: Use of peripheral blood- or bone marrow-derived progenitors for ischemic heart repair is a feasible option to induce neo-vascularization in ischemic tissues. These cells, named Endothelial Progenitors Cells (EPCs, have been extensively characterized phenotypically and functionally. The clinical efficacy of cardiac repair by EPCs cells remains, however, limited, due to cell autonomous defects as a consequence of risk factors. The devise of "enhancement" strategies has been therefore sought to improve repair ability of these cells and increase the clinical benefit. PRINCIPAL FINDINGS: Pharmacologic inhibition of histone deacetylases (HDACs is known to enhance hematopoietic stem cells engraftment by improvement of self renewal and inhibition of differentiation in the presence of mitogenic stimuli in vitro. In the present study cord blood-derived CD34(+ were pre-conditioned with the HDAC inhibitor Valproic Acid. This treatment affected stem cell growth and gene expression, and improved ischemic myocardium protection in an immunodeficient mouse model of myocardial infarction. CONCLUSIONS: Our results show that HDAC blockade leads to phenotype changes in CD34(+ cells with enhanced self renewal and cardioprotection.

Internet Renewable energy Information System (IRIS)

DEFF Research Database (Denmark)

Bäurle, Britta; Nielsen, Vilhjalmur; Ménard, Lionel

1999-01-01

Even though the Internet is now a widely accessible data source, the unorganised flood of information makes a specific request e.g. for renewable energy products inefficient. In addition, existing databases on renewable energies are often old and incomplete. The objective of IRIS has been...... to organise and retrieve renewable energy product information on the Internet instead of collecting it manually. Updating coincides with the self interestself-interest of manufacturers to present their latest renewable energy products on their own HTML documents. IRIS is based on a set of powerful tools...... and intends to find, extract, collect and index HTML documents with standardised META tags that are widely spread across web servers on the Internet. This paper presents the structure of IRIS, the software tools, and gives one example of how to categorise and prepare web-sites with product information...

A 100% renewable power system for Europe - Let the weather and physics decide!

DEFF Research Database (Denmark)

Greiner, Martin; Heide, Dominik; Rasmussen, Morten Grud

The design of sustainable energy systems is no longer only the domain of politics, economics and engineering. Mathematical physics is able to contribute with its generic understanding of everything. A new modeling approach is presented and applied to design a fully renewable European power system....... This approach is based on weather data with good spatio-temporal resolution, which is first converted into wind and solar power generation and then used to derive estimates on the optimal mix between the renewable resources and the storage needs.......The design of sustainable energy systems is no longer only the domain of politics, economics and engineering. Mathematical physics is able to contribute with its generic understanding of everything. A new modeling approach is presented and applied to design a fully renewable European power system...

Renewable vs. fossil electricity systems. A cost comparison. Power world 2050. Analysis of renewable, coal and gas-based electricity systems; Erneuerbare vs. fossile Stromsysteme. Ein Kostenvergleich. Stromwelten 2050. Analyse von Erneuerbaren, kohle- und gasbasierten Elektrizitaetssystemen

Energy Technology Data Exchange (ETDEWEB)

Graichen, Patrick; Kleiner, Mara Martha [Agora Energiewende, Berlin (Germany); Matthes, Felix Christian; Heinemann, Christoph [Oeko-Institut e.V. - Institut fuer Angewandte Oekologie, Berlin (Germany)

2017-01-15

The decarbonisation of the energy and, above all, the power system is the core component of any consistent climate protection strategy. For the electricity sector, this means, in the final analysis, the transition from a power supply based on lignite, hard coal, natural gas and other fossil fuels to one (almost) completely based on renewable energies by 2050. The fundamental technical feasibility of such a system, more than 90 percent of which would generate electricity from renewable energies, is no longer disputable today. The explanation for this is the partly rapid technological advances made in recent years, particularly those involving wind (on- and offshore) and solar energy, as well as the foreseeable further developments of central flexibility options (including flexible demand, battery storage and power-to-gas technologies). However, the question of the costs of this new electricity system has not yet been fully resolved. These cost calculations need to take into account, on the one hand, the total costs of an electricity system based on renewable energies and, on the other hand, the comparison to a power system that remains based on fossil fuels. Against this background, the present study provides a numerical analysis of the following questions: What are the technical and cost structures for a power system when 90 percent or more of the electricity is generated from renewable energies in 2050? How do the costs for different storage strategies (batteries vs. power-to-gas) differ? What technical, cost and emission structures result for a hypothetical fossil-based power system in 2050 if the further construction of electricity production plants based on wind and solar energy is immediately abandoned? How do the costs for various fossil-based power systems differ (conventional mix of lignite/hard coal/natural gas power plants vs. an electricity system based purely on natural gas)? For this purpose, a large number of model calculations with different

Renewable Substitutability Index: Maximizing Renewable Resource Use in Buildings

Directory of Open Access Journals (Sweden)

Ravi S. Srinivasan

2015-05-01

Full Text Available In order to achieve a material and energy balance in buildings that is sustainable in the long run, there is an urgent need to assess the renewable and non-renewable resources used in the manufacturing process and to progressively replace non-renewable resources with renewables. Such progressive disinvestment in the non-renewable resources that may be substituted with renewable resources is referred to as “Renewable Substitutability” and if implemented, this process will lead to a paradigm shift in the way building materials are manufactured. This paper discusses the development of a Renewable Substitutability Index (RSI that is designed to maximize the use of renewable resources in a building and quantifies the substitution process using solar emergy (i.e., the solar equivalent joules required for any item. The RSI of a building or a building component, i.e., floor or wall systems, etc., is the ratio of the renewable resources used during construction, including replacement and maintenance, to the building’s maximum renewable emergy potential. RSI values range between 0 and 1.0. A higher RSI achieves a low-energy building strategy promoting a higher order of sustainability by optimizing the use of renewables over a building’s lifetime from formation-extraction-manufacturing to maintenance, operation, demolition, and recycle.

Stability Enhancement of a Power System Containing High-Penetration Intermittent Renewable Generation

OpenAIRE

Morel, Jorge; Obara, Shin’ya; Morizane, Yuta

2015-01-01

This paper considers the transient stability enhancement of a power system containing large amounts of solar and wind generation in Japan. Following the Fukushima Daiichi nuclear disaster there has been an increasing awareness on the importance of a distributed architecture, based mainly on renewable generation, for the Japanese power system. Also, the targets of CO2 emissions can now be approached without heavily depending on nuclear generation. Large amounts of renewable generation leads to...

Optimal Design Of Renewable Energy Systemusing Genetic Algorithm Case Study In Parangtritis

Directory of Open Access Journals (Sweden)

SRI UTAMI

2016-08-01

Full Text Available Abstrak Andil pariwisata terhadap perkembangan regional sangat besar begitu juga andilnya terhadap permasalahan lingkungan.Untuk mengurangi aspek negatif terhadap lingkungan serta meningkatkan penghematan, sistem energi terbarukan menempati prioritas penting dalam bidang pariwisata. Konfigurasi optimal sistem energi terbarukan direncanakan menggunakan Algoritma Genetika. Penelitian ini dilakukan untuk mengoptimasi sistem energi terbarukan di Parangtritis, Kretek, Bantul, Jawa Tengah. Sistem yang dirancang terdiri dari sel surya dan turbin angin, sedangkan sistem penyimpanannya menggunakan baterai dan fuel cell. Algoritma ini meminimisasi fungsi objektif biaya total yang terdiri dari biaya investasi, biaya penggantian serta biaya operasi dan perawatan. Kehandalan sistem dievaluasi menggunakan indeks Equivalent Loss Factor (. Indeks ini memberikan informasi jumlah energi yang tidak dapat dipasok oleh sistem energi terbarukan. Hasil simulasi memperlihatkan jumlah optimal sistem energi terbarukan dicapai dengan jumlah sel surya sebanyak 3, baterai 48,turbin angin sebanyak 36, elektroliser sebanyak 3, tangki hidrogen 2 dan fuel cell sebanyak 8. Nilai ELF yang digunakan dalam penelitian ini adalah 0.01.  Abstract  Tourism has a massive contribution to regional development and shares environmental issues. Reducing reliances on fossil fuel, it is not still adequating energy consumption yet to cause development of renewable energy in crucial position for tourism desicition. An optimal configuration of renewable energy system was planned by Genetic Algorithm in this work. This research conducted to optimize renewable  energy system in Parangtritis, Kretek, Bantul Central Java. The system consisted of solar cells and wind turbines, andthe batteries and fuel cells were as storage system. The algorithm minimize objective function of total cost consisted of investment, replacement as well as operating and maintenance costs. A reability evaluation of the

Energy Storage Applications in Power Systems with Renewable Energy Generation

Science.gov (United States)

Ghofrani, Mahmoud

In this dissertation, we propose new operational and planning methodologies for power systems with renewable energy sources. A probabilistic optimal power flow (POPF) is developed to model wind power variations and evaluate the power system operation with intermittent renewable energy generation. The methodology is used to calculate the operating and ramping reserves that are required to compensate for power system uncertainties. Distributed wind generation is introduced as an operational scheme to take advantage of the spatial diversity of renewable energy resources and reduce wind power fluctuations using low or uncorrelated wind farms. The POPF is demonstrated using the IEEE 24-bus system where the proposed operational scheme reduces the operating and ramping reserve requirements and operation and congestion cost of the system as compared to operational practices available in the literature. A stochastic operational-planning framework is also proposed to adequately size, optimally place and schedule storage units within power systems with high wind penetrations. The method is used for different applications of energy storage systems for renewable energy integration. These applications include market-based opportunities such as renewable energy time-shift, renewable capacity firming, and transmission and distribution upgrade deferral in the form of revenue or reduced cost and storage-related societal benefits such as integration of more renewables, reduced emissions and improved utilization of grid assets. A power-pool model which incorporates the one-sided auction market into POPF is developed. The model considers storage units as market participants submitting hourly price bids in the form of marginal costs. This provides an accurate market-clearing process as compared to the 'price-taker' analysis available in the literature where the effects of large-scale storage units on the market-clearing prices are neglected. Different case studies are provided to

Loss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche.

Science.gov (United States)

Vanegas, Natalia-Del Pilar; Vernot, Jean-Paul

2017-01-01

Leukemic and mesenchymal stem cells interact in the leukemic microenvironment and affect each other differently. This interplay has also important implications for the hematopoietic stem cell (HSC) biology and function. This study evaluated human HSC self-renewal potential and quiescence in an in vitro leukemic niche without leukemic cells. A leukemic niche was established by co-culturing mesenchymal stem cells with a fresh conditioned medium obtained from a leukemic (REH) cell line. After 3 days, the REH-conditioned medium was removed and freshly isolated CD34+ at a density of up to 100,000 cells/ml were added to the leukemic niche. CD34+ cell evaluations (cell cycle, self-renewal gene expression and migration capacity) were performed after 3 further days of co-culture. Additionally, we preliminary investigated the soluble factors present in the leukemic niche and their effect on the mesenchymal stem cells. Statistical significance was assessed by Student's t test or the nonparametric test Kolmogorov-Smirnov. By co-culturing normal mesenchymal stem cells with the REH-conditioned medium we showed that hematopoietic stem cells, normally in a quiescent state, enter cell cycle and proliferate. This loss of quiescence was accompanied by an increased expression of Ki-67 and c-Myc, two well-known cell proliferation-associated markers. Two central regulators of quiescence GATA2 and p53 were also down regulated. Importantly, two genes involved in HSC self-renewal, Klf4 and the histone-lysine N -methyltransferase enzyme Ezh2, were severely affected. On the contrary, c-Kit expression, the stem cell factor receptor, was upregulated in hematopoietic stem cells when compared to the normal niche. Interestingly, mesenchymal stem cells incubated with the REH-conditioned medium stopped growing, showed a flattened morphology with the appearance of small vacuoles, and importantly, became positive for the senescence-associated beta-galactosidase activity. Evaluation of the leukemic

Regulated proteolysis of Trop2 drives epithelial hyperplasia and stem cell self-renewal via β-catenin signaling.

Science.gov (United States)

Stoyanova, Tanya; Goldstein, Andrew S; Cai, Houjian; Drake, Justin M; Huang, Jiaoti; Witte, Owen N

2012-10-15

The cell surface protein Trop2 is expressed on immature stem/progenitor-like cells and is overexpressed in many epithelial cancers. However the biological function of Trop2 in tissue maintenance and tumorigenesis remains unclear. In this study, we demonstrate that Trop2 is a regulator of self-renewal, proliferation, and transformation. Trop2 controls these processes through a mechanism of regulated intramembrane proteolysis that leads to cleavage of Trop2, creating two products: the extracellular domain and the intracellular domain. The intracellular domain of Trop2 is released from the membrane and accumulates in the nucleus. Heightened expression of the Trop2 intracellular domain promotes stem/progenitor self-renewal through signaling via β-catenin and is sufficient to initiate precursor lesions to prostate cancer in vivo. Importantly, we demonstrate that loss of β-catenin or Trop2 loss-of-function cleavage mutants abrogates Trop2-driven self-renewal and hyperplasia in the prostate. These findings suggest that heightened expression of Trop2 is selected for in epithelial cancers to enhance the stem-like properties of self-renewal and proliferation. Defining the mechanism of Trop2 function in self-renewal and transformation is essential to identify new therapeutic strategies to block Trop2 activation in cancer.

Power Electronics as Efficient Interface of Renewable Energy Sources

DEFF Research Database (Denmark)

Blaabjerg, Frede; Chen, Zhe; Kjær, Søren Bækhøj

2004-01-01

The global electrical energy consumption is steadily rising and consequently there is a demand to increase the power generation capacity. A significant percentage of the required capacity increase can be based on renewable energy sources. Wind turbine technology, as the most cost effective...... renewable energy conversion system, will play an important part in our future energy supply. But other sources like microturbines, photovoltaics and fuel cell systems may also be serious contributor to the power supply. Characteristically, power electronics will be an efficient and important interface...... to the grid and this paper will first briefly discuss three different alternative/ renewable energy sources. Next, various configurations of the wind turbine technology are presented, as this technology seems to be most developed and cost-effective. Finally, the developments and requirements from the grid...

Expression dynamics of self-renewal factors for spermatogonial stem cells in the mouse testis.

Science.gov (United States)

Sakai, Mizuki; Masaki, Kaito; Aiba, Shota; Tone, Masaaki; Takashima, Seiji

2018-04-16

Glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells (SSCs). Although GDNF is indispensable for the maintenance of SSCs, the role of FGF2 in the testis remains to be elucidated. To clarify this, the expression dynamics and regulatory mechanisms of Fgf2 and Gdnf in the mouse testes were analyzed. It is well known that Sertoli cells express Gdnf, and its receptor is expressed in a subset of undifferentiated spermatogonia, including SSCs. However, we found that Fgf2 was mainly expressed in the germ cells and its receptors were expressed not only in the cultured spermatogonial cell line, but also in testicular somatic cells. Aging, hypophysectomy, retinoic acid treatment, and testicular injury induced distinct Fgf2 and Gdnf expression dynamics, suggesting a difference in the expression mechanism of Fgf2 and Gdnf in the testis. Such differences might cause a dynamic fluctuation of Gdnf/Fgf2 ratio depending on the intrinsic/extrinsic cues. Considering that FGF2-cultured spermatogonia exhibit more differentiated phenotype than those cultured with GDNF, FGF2 might play a role distinct from that of GDNF in the testis, despite the fact that both factors are self-renewal factor for SSC in vitro.

Risoe energy report 5. Renewable energy for power and transport

Energy Technology Data Exchange (ETDEWEB)

Larsen, Hans; Soenderberg Petersen, L. (eds.)

2006-11-15

The global energy policy scene today is dominated by three concerns, namely security of supply, climate change and energy for development and poverty alleviation. This is the starting point for Risoe Energy Report 5 that addresses status and trends in renewable energy, and gives an overview of global driving forces for transformation of the energy systems in the light of security of supply, climate change and economic growth. More specifically status and trends in renewable energy technologies, for broader applications in off grid power production (and heat) will be discussed. Furthermore the report will address wider introduction of renewable energy in the transport sector, for example renewable based fuels, hybrid vehicles, electric vehicles and fuel cell driven vehicles. (au)

Risoe energy report 5. Renewable energy for power and transport

International Nuclear Information System (INIS)

Larsen, Hans; Soenderberg Petersen, L.

2006-11-01

The global energy policy scene today is dominated by three concerns, namely security of supply, climate change and energy for development and poverty alleviation. This is the starting point for Risoe Energy Report 5 that addresses status and trends in renewable energy, and gives an overview of global driving forces for transformation of the energy systems in the light of security of supply, climate change and economic growth. More specifically status and trends in renewable energy technologies, for broader applications in off grid power production (and heat) will be discussed. Furthermore the report will address wider introduction of renewable energy in the transport sector, for example renewable based fuels, hybrid vehicles, electric vehicles and fuel cell driven vehicles. (au)

Sp5 induces the expression of Nanog to maintain mouse embryonic stem cell self-renewal.

Science.gov (United States)

Tang, Ling; Wang, Manman; Liu, Dahai; Gong, Mengting; Ying, Qi-Long; Ye, Shoudong

2017-01-01

Activation of signal transducer and activator of transcription 3 (STAT3) by leukemia inhibitory factor (LIF) maintains mouse embryonic stem cell (mESC) self-renewal. Our previous study showed that trans-acting transcription factor 5 (Sp5), an LIF/STAT3 downstream target, supports mESC self-renewal. However, the mechanism by which Sp5 exerts these effects remains elusive. Here, we found that Nanog is a direct target of Sp5 and mediates the self-renewal-promoting effect of Sp5 in mESCs. Overexpression of Sp5 induced Nanog expression, while knockdown or knockout of Sp5 decreased the Nanog level. Moreover, chromatin immunoprecipitation (ChIP) assays showed that Sp5 directly bound to the Nanog promoter. Functional studies revealed that knockdown of Nanog eliminated the mESC self-renewal-promoting ability of Sp5. Finally, we demonstrated that the self-renewal-promoting function of Sp5 was largely dependent on its zinc finger domains. Taken together, our study provides unrecognized functions of Sp5 in mESCs and will expand our current understanding of the regulation of mESC pluripotency.

Loss of Asxl1 Alters Self-Renewal and Cell Fate of Bone Marrow Stromal Cell, Leading to Bohring-Opitz-like Syndrome in Mice

Directory of Open Access Journals (Sweden)

Peng Zhang

2016-06-01

Full Text Available De novo ASXL1 mutations are found in patients with Bohring-Opitz syndrome, a disease with severe developmental defects and early childhood mortality. The underlying pathologic mechanisms remain largely unknown. Using Asxl1-targeted murine models, we found that Asxl1 global loss as well as conditional deletion in osteoblasts and their progenitors led to significant bone loss and a markedly decreased number of bone marrow stromal cells (BMSCs compared with wild-type littermates. Asxl1−/− BMSCs displayed impaired self-renewal and skewed differentiation, away from osteoblasts and favoring adipocytes. RNA-sequencing analysis revealed altered expression of genes involved in cell proliferation, skeletal development, and morphogenesis. Furthermore, gene set enrichment analysis showed decreased expression of stem cell self-renewal gene signature, suggesting a role of Asxl1 in regulating the stemness of BMSCs. Importantly, re-introduction of Asxl1 normalized NANOG and OCT4 expression and restored the self-renewal capacity of Asxl1−/− BMSCs. Our study unveils a pivotal role of ASXL1 in the maintenance of BMSC functions and skeletal development.

Definition of a remuneration system for heat from renewable resources; Ausgestaltung einer Einspeiseverguetung fuer erneuerbare Waerme

Energy Technology Data Exchange (ETDEWEB)

Dettli, R.; Ott, W.; Philippen, D.; Umbricht, A.

2009-06-15

This report for the Swiss Federal Office of Energy (SFOE) deals with proposals for a remuneration system for heat obtained from renewable resources. Local and regional district heating systems cover around three percent of Swiss heating needs. The authors estimate that, if these systems were to be operated completely using renewable resources such as biomass, ambient heat and the renewable portion of heat from waste incineration, around seven per cent of needs could be met. Further, around 10,000 heating systems with a power of more than 350 kW could be operated with renewables. A further potential for the use of renewable heating resources can be found in wastewater treatment plants and industrial waste heat. Various obstacles and restraints on the use of renewable resources in the heating area are discussed. The idea of providing a cost-covering remuneration system for heat is discussed and compared with that for renewable electricity. The proposed system is discussed, which would provide investment subsidies, risk-coverage and project development subsidies. The report discusses the results of a market analysis and the differences to be found between the markets for electricity and heat. Existing promotional programs are noted and the aims of a possible remuneration system are discussed. A concept for a promotion program for renewable heat generation and the use of waste heat is introduced. The installations to be promoted and the amount of remuneration to be paid out are discussed. Finally, the costs and the effects of the proposed promotion scheme are discussed. A comprehensive appendix provides details on the proposed system and provides information on market volume, energy resources, networks and infrastructure, providers of heat energy, heat consumers and general conditions as far as factors such as pricing and legislation are concerned. Finally, the 'Climate Cent' foundation is commented on.

Targeting proapoptotic protein BAD inhibits survival and self-renewal of cancer stem cells.

Science.gov (United States)

Sastry, K S R; Al-Muftah, M A; Li, Pu; Al-Kowari, M K; Wang, E; Ismail Chouchane, A; Kizhakayil, D; Kulik, G; Marincola, F M; Haoudi, A; Chouchane, L

2014-12-01

Emerging evidence suggests that the resistance of cancer stem cells (CSC) to many conventional therapies is one of the major limiting factors of cancer therapy efficacy. Identification of mechanisms responsible for survival and self-renewal of CSC will help design new therapeutic strategies that target and eliminate both differentiated cancer cells and CSC. Here we demonstrated the potential role of proapoptotic protein BAD in the biology of CSC in melanoma, prostate and breast cancers. We enriched CD44(+)/CD24(-) cells (CSC) by tumorosphere formation and purified this population by FACS. Both spheres and CSC exhibited increased potential for proliferation, migration, invasion, sphere formation, anchorage-independent growth, as well as upregulation of several stem cell-associated markers. We showed that the phosphorylation of BAD is essential for the survival of CSC. Conversely, ectopic expression of a phosphorylation-deficient mutant BAD induced apoptosis in CSC. This effect was enhanced by treatment with a BH3-mimetic, ABT-737. Both pharmacological agents that inhibit survival kinases and growth factors that are involved in drug resistance delivered their respective cytotoxic and protective effects by modulating the BAD phosphorylation in CSC. Furthermore, the frequency and self-renewal capacity of CSC was significantly reduced by knocking down the BAD expression. Consistent with our in vitro results, significant phosphorylation of BAD was found in CD44(+) CSC of 83% breast tumor specimens. In addition, we also identified a positive correlation between BAD expression and disease stage in prostate cancer, suggesting a role of BAD in tumor advancement. Our studies unveil the role of BAD in the survival and self-renewal of CSC and propose BAD not only as an attractive target for cancer therapy but also as a marker of tumor progression.

Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-κB signaling pathways.

Science.gov (United States)

Ogura, Yuji; Mishra, Vivek; Hindi, Sajedah M; Kuang, Shihuan; Kumar, Ashok

2013-12-06

Satellite cell self-renewal is an essential process to maintaining the robustness of skeletal muscle regenerative capacity. However, extrinsic factors that regulate self-renewal of satellite cells are not well understood. Here, we demonstrate that TWEAK cytokine reduces the proportion of Pax7(+)/MyoD(-) cells (an index of self-renewal) on myofiber explants and represses multiple components of Notch signaling in satellite cell cultures. The number of Pax7(+) cells is significantly increased in skeletal muscle of TWEAK knock-out (KO) mice compared with wild-type in response to injury. Furthermore, Notch signaling is significantly elevated in cultured satellite cells and in regenerating myofibers of TWEAK-KO mice. Forced activation of Notch signaling through overexpression of the Notch1 intracellular domain (N1ICD) rescued the TWEAK-mediated inhibition of satellite cell self-renewal. TWEAK also activates the NF-κB transcription factor in satellite cells and inhibition of NF-κB significantly improved the number of Pax7(+) cells in TWEAK-treated cultures. Furthermore, our results demonstrate that a reciprocal interaction between NF-κB and Notch signaling governs the inhibitory effect of TWEAK on satellite cell self-renewal. Collectively, our study demonstrates that TWEAK suppresses satellite cell self-renewal through activating NF-κB and repressing Notch signaling.

Ireland's pathway towards a 100% renewable energy-system

DEFF Research Database (Denmark)

Connolly, David; Leahy, Martin; Lund, Henrik

2009-01-01

Ireland has an abundant supply of renewable energy, a dangerous reliance on imported fossil fuels, as well as very demanding energy and CO2 targets to reach. All of these indicate that a major alternation to the current energy system is necessary, especially an increase in the utilisation of rene...

Sizing Hydrogen Energy Storage in Consideration of Demand Response in Highly Renewable Generation Power Systems

Directory of Open Access Journals (Sweden)

Mubbashir Ali

2018-05-01

Full Text Available From an environment perspective, the increased penetration of wind and solar generation in power systems is remarkable. However, as the intermittent renewable generation briskly grows, electrical grids are experiencing significant discrepancies between supply and demand as a result of limited system flexibility. This paper investigates the optimal sizing and control of the hydrogen energy storage system for increased utilization of renewable generation. Using a Finnish case study, a mathematical model is presented to investigate the optimal storage capacity in a renewable power system. In addition, the impact of demand response for domestic storage space heating in terms of the optimal sizing of energy storage is discussed. Finally, sensitivity analyses are conducted to observe the impact of a small share of controllable baseload production as well as the oversizing of renewable generation in terms of required hydrogen storage size.

Human Capacity Building in Energy Efficiency and Renewable Energy System Maintenance for the Yurok Tribe

Energy Technology Data Exchange (ETDEWEB)

Engel, R. A.' Zoellick, J J.

2007-07-31

From July 2005 to July 2007, the Schatz Energy Research Center (SERC) assisted the Yurok Tribe in the implementation of a program designed to build the Tribe’s own capacity to improve energy efficiency and maintain and repair renewable energy systems in Tribal homes on the Yurok Reservation. Funding for this effort was provided by the U.S. Department of Energy’s Tribal Program under First Steps grant award #DE-FG36-05GO15166. The program’s centerpiece was a house-by-house needs assessment, in which Tribal staff visited and conducted energy audits at over fifty homes. The visits included assessment of household energy efficiency and condition of existing renewable energy systems. Staff also provided energy education to residents, evaluated potential sites for new household renewable energy systems, and performed minor repairs as needed on renewable energy systems.

Integration of supercapacitive storage in renewable energy system to compare the response of two level and five level inverter with RL type load

Science.gov (United States)

Jana, Suman; Biswas, Pabitra Kumar; Das, Upama

2018-04-01

The analytical and simulation-based study in this presented paper shows a comparative report between two level inverter and five-level inverter with the integration of Supercapacitive storage in Renewable Energy system. Sometime dependent numerical models are used to measure the voltage and current response of two level and five level inverter in MATLAB Simulink based environment. In this study supercapacitive sources, which are fed by solar cells are used as input sources to experiment the response of multilevel inverter with integration of su-percapacitor as a storage device of Renewable Energy System. The RL load is used to compute the time response in MATLABSimulink based environment. With the simulation results a comparative study has been made of two different level types of inverters. Two basic types of inverter are discussed in the study with reference to their electrical behavior. It is also simulated that multilevel inverter can convert stored energy within supercapacitor which is extracted from Renewable Energy System.

Hydro Solar 21- A building energetic demand providing system based on renewable energies and hydrogen; Hydro Solar 21- Energias renovables e hidrogeno para el abastecimiento energetico de un edificio

Energy Technology Data Exchange (ETDEWEB)

Renilla Collado, R.; Ortega Izquierdo, M.

2008-07-01

Hydro Solar 21 is an energy innovation Project carried out in Burgos City to develop an energy production system based on renewable energies to satisfy light and air condition requirements of a restored building. Nocturnal light demand is satisfied with hydrogen consumption in fuel cells. This hydrogen is produced with an energy renewable system made up of two wind turbine generators and a photovoltaic system. The air conditioning demand is satisfied with an adsorption solar system which produces cold water using thermal solar energy. (Author) 8 refs.

Electrochemical Systems for Renewable Energy Conversion from Salinity and Proton Gradients

OpenAIRE

Morais, William G.; Lima, Gilberto; Gomes, Wellington J. A. S.; Huguenin, Fritz

2018-01-01

Ever-rising energy demand, fossil fuel dependence, and climate issues have harmful consequences to the society. Exploring clean and renewable energy to diversify the world energy matrix has become an urgent matter. Less explored or unexplored renewable energy sources like the salinity and proton gradient energy are an attractive alternative with great energy potential. This paper discusses important electrochemical systems for energy conversion from natural and artificial concentration gradie...

Trends in Power Electronics and Control of Renewable Energy Systems

DEFF Research Database (Denmark)

Blaabjerg, Frede; Iov, Florin; Kerekes, Tamas

2010-01-01

term) based energy sources to renewable energy sources. Another is to use high efficient power electronics in power generation, power transmission/distribution and end-user application. This paper discuss trends of the most emerging renewable energy sources, wind energy and photovoltaics, which...... by means of power electronics are changing the future electrical infrastructure but also contributes steadily more to non-carbon based electricity production. Most focus is on the power electronics technologies used. In the case of photovoltaics transformer-less systems are discussed as they have...

A Transition Strategy from Fossil Fuels to Renewable Energy Sources in the Mexican Electricity System

Directory of Open Access Journals (Sweden)

Juan J. Vidal-Amaro

2018-03-01

Full Text Available Renewable energy sources exploitation acquires special importance for creating low-carbon energy systems. In Mexico a national regulation limits the fossil fuel-based electricity generation to 65%, 60% and 50% by years 2024, 2030 and 2050 respectively. This study evaluates several scenarios of renewables incorporation into the Mexican electricity system to attend those targets as well as a 75% renewables-based electricity share target towards a 100% renewable system. By its size, the Mexican electricity system, with a generation of 260.4 TWh/year (85% based on fossil fuels, can be regarded as an illustrating reference. The impact of increasing amounts of wind, photovoltaic solar, biomass, biogas, geothermal, hydro and concentrating solar power on the system’s capacity to attend demand on a one-hour timescale resolution is investigated utilizing the EnergyPLAN model and the minimum total mix capacity method. Possible excess of electricity production is also assessed. For every target year, a solution is obtained corresponding to the combination resulting in the minimum total generation capacity for the electricity system. A transition strategy to a system with a high share of renewables-based electricity is designed where every transition step corresponds to the optimal energy mix for each of the target years.

Financial incentives to promote renewable energy systems in European electricity markets: a survey

International Nuclear Information System (INIS)

Haas, R.; Huber, C.; Wohlgemuth, N.

2001-01-01

Renewable energy systems may contribute to sustainable development. Therefore, one of the challenges for energy policy is to ensure that renewable energy options have a fair opportunity to compete with other supply resources. This paper presents a survey on promotion mechanisms to enhance the market penetration of renewable energies in European electricity markets. Strategies include rebates and tax incentives, regulated rates, system benefit charges, bidding-oriented mechanisms and various types of green pricing programs. The paper concludes that efficient promotion mechanisms should focus on incentives per kWh generated rather than on rebates on the investment in generating capacity (kW), and that there is no one single program type which has the best application to the promotion of all renewable technologies. For example, enhanced buy-back rates work as a dissemination strategy for wind energy but they do not work for photovoltaics. (author)