PUDRICIÓN BASAL CAUSADA POR Phytophthora capsici EN PLANTAS DE CHILE TRATADAS CON VERMICOMPOST

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Lidieth Uribe-Lor\\u00EDo

2014-01-01

Full Text Available Pudrición basal causada por Phytophthora capcisi en plantas de chile tratadas con vermicompost. El objetivo de este trabajo fue evaluar a nivel de invernadero el efecto de vermicompost sobre la incidencia y severidad de Phytophthora capcisi en plantas de chile (Capsicum annuum. Esta investigación se realizó entre febrero y marzo del 2012 en el Centro de Investigaciones Agronómicas, Sabanilla, San José, Costa Rica. Se utilizó un diseño completamente al azar con cuatro repeticiones y ocho plantas por unidad experimental en un arreglo factorial con los factores de dosis (0, 25% y 50% v/v vermicompost:suelo e inoculación (0 y 500 zoosporas por gramo de suelo. Se trasplantaron plántulas de 35 días, que fueron inoculadas dos semanas después del trasplante. La aplicación de vermicompost provocó un aumento significativo del peso fresco foliar y del peso seco foliar y radical; a mayor dosis, mayor fue el incremento. Las plantas de todos los tratamientos que incluyeron inoculación con P. capsici presentaron síntomas de la enfermedad en la raíz. Los valores de incidencia y severidad fueron mayores para el tratamiento con 50% de abono. En este tratamiento se presentaron síntomas de marchitez. La ausencia de diferencias en las variables de peso fresco y seco entre los tratamientos inoculados y sin inocular, a los que se adicionó abono al 25%, sugiere que esta dosis podría compensar el daño causado por el patógeno. Se observó una menor concentración de nutrimentos en los tejidos de las plantas inoculadas lo que indica que el daño causado a la raíz pudo haber afectado la adquisición de nutrimentos.

Estado de la moniliasis del cacao causada por Moniliophthora roreri en Colombia

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Javier Correa Álvarez

2014-12-01

Full Text Available La moniliasis es una enfermedad fúngica que ataca el cultivo de cacao, causada por el basidiomycete Moniliophthora roreri. Está presente en la mayoría de los países latinoamericanos y se adapta a diversidad de ambientes. En Colombia la enfermedad es devastadora y las estrategias de control tradicional han generado resultados colaterales como el fortalecimiento de la resistencia genética de cepas del hongo en algunas regiones. En esta revisión, se recopilan los aspectos biológicos y agronómicos más relevantes del hongo, entre ellos, origen y distribución, ciclo de vida, formas de control y trabajos de investigación realizados con el fin de entender la genética y evolución de este patógeno. Finalmente, se propone incrementar el número de estudios en investigación básica, con miras a entender cómo ha sido la evolución de su genoma en hábitats que favorecen la variación genética. Con este conocimiento se podría avanzar en programas biotecnológicos de control y prevención de la enfermedad.

Infecções intramamárias causadas por Staphylococcus aureus e suas implicações em paúde pública

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Fagundes,Helena; Oliveira,Carlos Augusto Fernandes

2004-01-01

Neste trabalho, são apresentados os principais problemas decorrentes das infecções intramamárias (mastites) causadas por Staphylococcus aureus e as conseqüências para a saúde humana da veiculação de suas toxinas através do leite. o S. aureus destaca-se como um dos microorganismos mais importantes que podem ser transmitidos através dos alimentos. Assim, discute-se a possibilidade de veiculação de gastroenterite estafilocócica, não somente através do consumo de leite cru contaminado, mas também...

Ptose palpebral causada por Paquidermoperiostose

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Patricia Regina de Pinho Tavares

2014-08-01

Full Text Available A paquidermoperiostose é uma síndrome caracterizada por acometimento cutâneo e ósseo, e em alguns casos ocorre comprometimento palpebral leve. É uma síndrome rara, idiopática ou hereditária, com provável herança autossômica dominante de penetrância variável. Descreve-se o caso de um paciente com ptose grave por paquidermoperiostose elucidando sua fisiopatologia e conduta cirúrgica aplicada.

Relato de Caso: Dermatite de Contato Causada por Arruda (Ruta graveolens L.)

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Cortez, Lúcia Elaine Ranieri; Cortez, Diógenes Aparício Garcia

2008-01-01

Um homem de 26 anos desenvolveu uma reação fototóxica em suas pernas, após utilizar as partes aéreas frescas da arruda (Ruta graveolens L.), para repelir pernilongos, depois de se expor ao sol. A hipersensibilidade foi causada devido às furanocumarinas (psoralenos) presentes no óleo essencial da arruda.

A FERRUGEM ASIÁTICA DA SOJA CAUSADA POR Phakopsora pachyrhizi Sydow e Sydow

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Felipe Rafael Garcés Fiallos

2012-01-01

Full Text Available Os Estados Unidos são o principal produtor mundial de soja, seguido pelo Brasil. A cultura da soja é afetada por 47 doenças já relatadas, entre elas a ferrugem asiática causada pelo fungo biotrófico Phakopsora pachyrhizi Sydow e Sydow. Esta doença pode atingir até 100% de dano e causa perdas no Brasil estimadas em 737,453,718 dólares ao ano. Em função de sua importância, faz-se oportuno uma revisão bibliográfica sobre esta doença, que abordará a sua ocorrência a nível mundial, a sintomatologia, os hospedeiros, a taxonomia e a etiologia do agente causal, efeitos dos fatores climáticos sobre a doença, ciclo biológico do patógeno, progresso e análise temporal, redução na área foliar e nos componentes de rendimento da cultura da soja e as estratégias de manejo integrado da enfermidade. Foi objetivo desta revisão recopilar as informações mais relevantes sobre a ferrugem asiática de modo o mais explicativo e entendível possível para o leitor.

Primeira ocorrência de ferrugem em capim-limão causada por Puccinia cymbopogonis no Brasil First report of the lemongrass rust fungi caused by Puccinia cymbopogonis in Brazil

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João Batista Vida

2006-03-01

Full Text Available È registrada a primeira ocorrência de ferrugem em capim-limão (Cymbopogon citratus (DC. Stapf, causada por Puccinia cymbopogonis Mass., no Brasil, Estado do Paraná.It is reported the first occurrence of the lemongrass (Cymbopogon citratus (DC. Stapf rust fungi caused by Puccinia cymbopogonis Mass. in Brazil, State of Paraná.

Podridão de raízes causada por Pythium aphanidermatum, em cultivares de alface produzidas em sistema hidropônico

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Zayame Vegette Pinto

2011-12-01

Full Text Available A podridão de raízes, causada por Pythium aphanidermatum e outras espécies de Pythium, é a principal doença da alface cultivada em sistemas hidropônicos no Brasil. O presente trabalho teve como objetivo avaliar quatro cultivares comerciais de alface em relação à sensibilidade a podridão de raízes, causada por P. aphanidermatum. Os estudos foram realizados em placas de Petri contendo ágar-água com plântulas de alface das cultivares crespa (Vera e Verônica e lisa (Regina e Elisa, infestadas ou não com o patógeno. Com as mesmas cultivares foram realizados quatro experimentos em sistemas hidropônicos (Nutrient Film Technique, sendo dois em estufa coberta com plástico e sombrite e dois em estufa coberta apenas com plástico. As plântulas, infectadas ou não com P. aphanidermatum, foram transplantadas para os sistemas infestados ou não. Foi avaliada a severidade da doença e o desenvolvimento das plantas. Todas as cultivares foram suscetíveis à podridão de raízes nos experimentos realizados in vitro e in vivo. A presença do sombrite não reduziu a podridão de raízes em cultivares de alface produzidas no sistema hidropônico. A cultivar Regina apresentou maior massa de matéria seca da parte aérea e das raízes, na presença ou ausência do patógeno, sendo a mais indicada para o cultivo hidropônico na época mais quente do ano.

Infección pulmonar por Mycobacterium avium en paciente VIH/SIDA: Primer reporte en Perú

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Juan Carrasco

Full Text Available El complejo Mycobacterium avium (MAC es un patógeno que se encuentra en el medioambiente y causa infecciones tanto en pacientes inmunocompetentes como inmunocomprometidos. Se presenta el caso de un paciente VIH positivo varón de 38 años infectado por P. jirovecii y aparentemente infectado por Mycobacterium tuberculosis desde el año 2009, el cual fue tratado con antibioticoterapia para pneumocistosis y terapia antituberculosis (TB logrando mejoría parcial. En el año 2012 se le realizó nuevamente examen de cultivo y un nuevo tratamiento anti TB, frente a la sospecha de estar en presencia de una cepa de TB multidrogorresistente se recomienda realizar la identificación micobacteriana. El examen de cultivo fue positivo y el resultado genotípico resultó positivo para MAC. Se reporta el primer caso de un paciente VIH/SIDA con infección pulmonar por MAC en el Perú, así como una breve revisión de los aspectos epidemiológicos, clínicos y de tratamiento

Parinaud syndrome caused by Bartonella henselae: case report Síndrome de Parinaud causada por Bartonella henselae: relato de caso

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Carlos Alberto Yamashita

1996-12-01

Full Text Available The authors report a case of Parinaud syndrome (conjunctivitis with pre-auricular satellite adenitis caused by Bartonella henselae, the etiologic agent of Cat Scratch Disease. The etiologic assessment of this case was performed by serum indirect immunofluorescence reaction and allowed for a better therapeutics and follow up, avoiding ineffective antibiotics and surgical interventions.Os autores relatam um caso do Síndrome de Parinaud (conjuntivite com adenite satélite pré-auricular causada por Bartonella henselae, o agente etiológico da Doença da Arranhadura do Gato. O esclarecimento etiológico deste caso foi feito através da reação de imunofluorescência indireta o que permitiu um adequado enfoque terapêutico e acompanhamento do paciente, evitando o uso de antibióticos inadequados e intervenções cirúrgicas.

Constrição campimétrica causada por vigabatrin Visual field constriction caused by vigabatrin

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Mário Luiz Ribeiro Monteiro

2000-10-01

Full Text Available Objetivo: Descrever dois pacientes que apresentaram constrição campimétrica importante e determinar a origem desta alteração visual causada pelo uso de vigabatrin, um anticonvulsivante que atua aumentando a concentração do ácido gama-aminobutírico no sistema nervoso central. Métodos: Os pacientes foram submetidos a exame e neuroftalmológico completo, avaliação cuidadosa da retina, campo visual com perímetro de Goldmann, tomografia computadorizada de crânio e órbitas, eletrorretinograma (ERG, imagem por ressonância magnética (1 paciente e potencial visual evocado (1 paciente. Resultados: A acuidade visual se mostrou preservada; o primeiro paciente apresentava campo visual restrito à área central de 20 a 30 graus centrais e o segundo uma constrição leve a moderada. Os exames de neuroimagem foram normais assim como o potencial visual evocado. Observou-se palidez discreta de papila em 3 olhos estudados e estreitamento arteriolar muito discreto dos vasos retinianos. O ERG revelou alterações importantes caracterizada por redução da onda b (caso 1 e dos potenciais oscilatórios (caso 2. Conclusões: As alterações visuais causadas pelo vigabatrin são devidas a uma alteração retiniana, envolvendo provavelmente as células amácrimas, bipolares e ganglionares mas não se acompanham de uma alteração significativa à fundoscopia. Pacientes recebendo esta medicação devem ser seguidos de perto especialmente através da avaliação do campo visual central e periférico.Purpose: To describe two patients who presented with severe campimetric constriction and to determine the source of visual symptoms caused by vigabatrin, an anticonvulsant medication that increases brain gamma-aminobutyric acid. Methods: Patients were submitted to a complete neuroophthalmologic evaluation as well as a careful retina examination, Goldmann perimetry, computerized tomography of the brain and orbits, electroretinograms (ERG, magnetic ressonance

Estudio retrospectivo de la prevalencia de Fasciola hepática y análisis de pérdidas causadas por decomisos de hígados a nivel de centro de faenamiento

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Buestán Sarmiento, Priscila Estefanía

2017-01-01

Este estudio determinó la prevalencia de Distomatosis hepática en bovinos y porcinos causada por el parásito trematodo Fasciola hepática, y estableció las pérdidas económicas ocasionadas por decomisos de hígados post faenamiento; mediante un análisis económico de pérdidas y un estudio retrospectivo de los años 2015 y 2016 a través de la información de los Registros Mensuales de Inspección Veterinaria que maneja el Centro de faenamiento. This study determined the prevalence of hepatic disto...

La necrosis bacteriana de la vid, causada por xylophilus ampelinus. Detección serológica, distribución en Aragón y sensibilidad varietal.

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CAMBRA ALVAREZ, MIGUEL

2011-01-01

La necrosis bacteriana de la vid, causada por la bacteria de cuarentena Xylophilus ampelinus, es una enfermedad de difícil control químico y que ha causado en España importantes pérdidas económicas. Actualmente, es endémica en distintas zonas de Aragón y Galicia y se han identificado focos aislados en La Rioja. Ante la carencia de métodos sensibles y específicos de detección de esta bacteria que se puedan utilizar para el análisis rutinario, se ha puesto a punto un método de ex...

ANTRACNOSE DO CAQUIZEIRO CAUSADA POR Colletotrichum horii: INCIDÊNCIA EM RAMOS, FOLHAS, FLORES E FRUTOS EM CAMPO

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RENATO REZENDE YOUNG BLOOD

2015-06-01

Full Text Available O objetivo deste trabalho foi avaliar a incidência em várias partes da planta e o desenvolvimento da antracnose do caquizeiro causada por Colletotrichum horiinas cultivares Fuyu e Kakimel produzidas sob os sistemas orgânico e convencional, respectivamente, nas safras de 2010/2011 e 2011/2012, em Campina Grande do Sul, Paraná, Brasil. Em cada pomar, 10 plantas foram selecionadas aleatoriamente, e 10 ramos do ano por planta, distribuídos ao redor dacopa, foram marcados para as avaliações de incidência de antracnose em ramos e folhas, a cada 15 dias. A quantificação de frutos caídos com ou sem sintomas da doença foi a cada 15 dias, durante o período de crescimento de frutos até a colheita, relacionada com a produção total. C. horii em flores foi avaliada em campo e também após incubação no laboratório. Infecção latente foi observada em frutos verdes coletados aos 90; 120 e 150 dias após a floração. Em ambas as safras, observou-se a queda de frutos com sintomas de antracnose entre os meses de janeiro e abril. C. horii é capaz de infectar flores, permanecer latente e provocar sintomas de antracnose em frutos imaturos. Frutos colhidos sadios apresentaram sintomas em pós-colheita após a incubação.

Perdas causadas por Coccus viridis (Hemiptera: Coccidae em mudas de Coffea arabica L.

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Flávio Lemes Fernandes

2009-08-01

Full Text Available Coccus viridis (Green danifica plantas jovens e adultas de Coffea arabica Linnaeu. No entanto, nada se sabe sobre a magnitude dos danos causados por esta praga. Assim, este trabalho teve por objetivo estudar as relações entre o ataque de C. viridis e as perdas causadas por este inseto a C. arabica. Este trabalho foi conduzido em casa de vegetação na Universidade Federal de Viçosa. Foram utilizadas sementes da linhagem IAC 15 da variedade “Catuaí vermelho” de café (C. arabica. Para a confecção dos tratamentos esta praga foi criada em casa de vegetação separada do experimento. Os tratamentos foram: plantas infestadas e não infestadas por adultos e ninfas da cochonilha verde. As plantas foram nutridas com solução nutritiva. Durante 110 dias foram avaliados: números de adultos e de ninfas de primeiro, segundo e terceiro ínstares, área foliar, diâmetro do caule, altura das plantas em todas repetições. No final do experimento avaliou-se o peso das raízes, caule, folhas e total. Os pesos das raízes, matéria seca total, área foliar e diâmetro do caule de plantas não atacadas por C. viridis superaram em 1,31; 1,41; 1,50 e 8,93 vezes, respectivamente o peso de plantas atacadas. As variáveis selecionadas foram: diâmetro do caule (cm, área foliar (cm², peso de raízes (g, ninfas, adultos e total das cochonilhas. Concluindo que a planta de C. arabica é afetada de forma diferente entre seus órgãos e que a ninfa de terceiro ínstar e adultos são as fases que mais causam danos a C. arabica.Losses Caused by Coccus viridis (Green (Hemiptera: Coccidae on Seedlings of Coffea arabica L.Abstract. Coccus viridis (Green cause losses on seedling and old plants of Coffea arabica (Green. However, nothing is known about of the damages caused by this pest. Thus, the objective of this work was to evaluate the relations between atack of C. viridis and the losses caused by this insect. on C. arabica. This work was conduced in greenhouse at

Injúrias causadas por mosca-das-frutas sul-americana em cultivares de videira

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Marcelo Zart

2011-01-01

Full Text Available A mosca-das-frutas sul-americana, Anastrepha fraterculus (Wiedemann (Diptera: Tephritidae, é uma das principais pragas da fruticultura no Brasil. O objetivo deste estudo foi identificar a suscetibilidade quanto à queda e deformação de bagas de diferentes cultivares/espécies de uva pela injúria causada pela oviposição de A. fraterculus. O trabalho foi realizado em vinhedos das cultivares 'Cabernet Sauvignon' (Vitis vinifera, 'Moscato Embrapa' (75% de V. vinifera e 'Isabel' (Vitis labrusca, localizados em Bento Gonçalves (RS, na safra 2006/2007. Dois casais de A. fraterculus foram confinados por cacho, em gaiolas de tecido voile, durante os estádios fenológicos de grão ervilha, início da compactação, início da maturação e maturação plena dos cachos. Queda significativa de bagas devido ao ataque de A. fraterculus foi registrada na cultivar 'Cabernet Sauvignon' quando a infestação foi realizada nos estádios fenológicos de grão ervilha e início da compactação de cacho; estádios de grão ervilha em 'Moscato Embrapa'; e nos estádios de grão ervilha, início da compactação e início da maturação do cacho na cultivar 'Isabel'. Foi registrada deformação de bagas quando as infestações foram realizadas nos estádios de grão ervilha e início da compactação de cacho em 'Cabernet Sauvignon', 'Moscato Embrapa' e 'Isabel'. O desenvolvimento larval até a fase de pupa foi observado somente na cultivar 'Moscato Embrapa'.

Granuloma macular por tuberculose sem manifestação pulmonar

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Albert Costa Rebello

2015-08-01

Full Text Available RESUMO Tuberculose é uma doença infecciosa causada pelo Mycobacterium tuberculosis, também conhecido como bacilo de Koch. O principal sítio de acometimento é o pulmonar, porém o bacilo pode disseminar-se por via linfo-hematogênica para outros órgãos, dentre eles o olho. A incidência de tuberculose ocular é de 1 a 2% dos casos extrapulmonares. Os autores apresentam um caso clínico de um paciente do sexo feminino de 28 anos que procura atendimento médico devido à redução da acuidade visual em olho esquerdo há 7 dias. Apresentava a melhor acuidade visual corrigida no olho acometido de 20/200 e no olho contralateral de 20/20. Na fundoscopia era evidenciado um granuloma em área macular do olho esquerdo, com edema e hemorragia intrarretiniana adjacente. Após investigação diagnóstica, a paciente foi tratada com esquema antibiótico para tuberculose durante 6 meses, obtendo regressão do granuloma e melhora da acuidade visual deste olho para 20/50.

[Cutaneous infection by Mycobacterium fortuitum]  Infeccion cutanea por Mycobacterium fortuitum

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Verónica Rotela

2017-10-01

Full Text Available Mycobacteria are aerobic, non-spore forming, gram positive, acid-fast bacilli, which affect skin, subcutaneous tissue, and other organs and systems. Mycobacterium fortuitum produces cellulitis, abscesses, papules-pustules, nodules and ulcers with serosanguinolent, purulent material, and subcutaneous necrosis. A 61-year-old woman, presents a case of two months of evolution that begins with reddish grain from an insect sting. After immersion in the Mexican Sea, it worsens, increases in quantity, is blistered and has brownish secretion; Physical examination shows erythematous plaque, with punctate orifices with hematic and meliceric crusts; Pustules and satellite papules, on the anterior aspect of the right leg. Histopathology: Suppurative dermal granulomas, centered by acute leukocyte infiltrate, with liquefactive tissue necrosis, surrounded by chronic inflammation with macrophages, plasma cells, lymphocytes, multinucleated giant cells. The first skin culture returns negative; in the second skin culture, fast-growing, non-pigmented atypical mycobacteria. Molecular detection is performed by Polymerase Chain Reaction: Mycobacterium fortuitum. Treatment with Ciprofloxacin 500 mg every 12 hours, with resolution of the table to the eighth month. A case of cutaneous infection by Mycobacterium fortuitum, related to the immersion in the sea and corals, whose diagnostic process has been difficult and was achieved by techniques of advanced molecular biology.

Mesa en cuello por Mycobacterium tuberculosis en un paciente infectado por HIV A case of cervical mass due to mycobacterium tuberculosis in an HIV infected patient

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Mónica Soto

1995-02-01

Full Text Available Se presenta el caso de un paciente de sexo masculino con diagnóstico de infección por HIV quien presentó una masa en cuello de 7 x 10 cm, dolorosa, de consistencia dura, adherida, no pulsátil, con edema de la piel adyacente y sin otros signos asociados. La evolución fue de 6 meses. Los estudios de la masa y el esputo dieron como resultado el aislamiento de M. tuberculosis, lo que permitió un adecuado enfoque terapéutico y por consiguiente una respuesta clínica satisfactoria

Eficácia do moxalactam no tratamento de meningites purulentas causadas por Haemophilus infuenzae e Neisseria meningitidis Efficacy of moxalactam in the treatment of purulent meningitis caused by Haemophilus influenzae and Neisseria meningitidis

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Hagamenon R. da Silva

1984-03-01

Full Text Available Foi avaliada a eficácia do moxalactam no tratamento de meningites em crianças, causadas por H. influenzae (27 casos e N.meningitidis (6 casos. Dos 33 doentes tratados na dose de 100mg/Kg de peso (dose de ataque e 50mg de 12/12 horas por via venosa, 32 curaram-se. A tolerância ao produto foi muito boa, havendo alterações transitórias de transaminases e fosfatase alcalina; em um caso, houve hematoma posapendectomia, provavelmente relacionado ao uso deste antibiotico. Os níveis séricos e liquóricos do produto foram elevados; as concentrações no liquor excederam de muito a concentração bactericida mínima dos germes infectantes. O moxalactam se mostrou seguro e eficaz como terapia primária da meningite causada por H. influenzae e N.meningitidis em crianças.The clinical efficacy and safety of Moxalactam in purulent bacterial meningitis in children caused by H. influenzae (27 patients and N. meningitidis (6 patients was tested in a randon uncontrolled study. Clinical response was considered excelent, with cure of 32 of 33 patients. High levels of Moxalactam were achieved in the blood and cerebro-spinal fluid, with concentrations largely exceeding the minimum bacterial concentration (MIC for the infecting organisms. Tolerance was considered good, with only transient increases of transaminases and alkaline phosphatase in some patients; also, one patient developed a wound hematoma possibly related to Moxalactam therapy.

Enfermedad por Mycobacterium simiae y "Mycobacterium sherrisii" en la Argentina Disease due to Mycobacterium simiae and "Mycobacterium sherrisii" in Argentina

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Lucía Barrera

2010-08-01

Full Text Available Se presenta información reunida retrospectivamente sobre casos de micobacteriosis originados por Mycobacterium simiae (n = 4 y "M. sherrisii" (n = 6. Los casos ocurrieron entre pacientes con sida (n = 6, historia de silicosis (n = 2 o tuberculosis previa (n = 1. Un caso se perdió luego de diagnosticado y nueve fueron tratados con esquemas terapéuticos basados en claritromicina, etambutol y quinolonas. La respuesta fue muy pobre: cinco pacientes fallecieron (cuatro eran HIV positivos, tres permanecieron crónicos y sólo uno curó. Estas micobacterias originaron 2.1% de los casos de micobacteriosis registrados en un período de ocho años. La distinción de estas micobacterias raras de otras más frecuentes por métodos moleculares rápidos, parece ser clínicamente útil para advertir sobre la dificultad que puede presentar el tratamiento. Sin embargo, la diferenciación genotípica entre M. simiae y "M. sherrisii" parecería no ser clínicamente relevante, dado que no quedaron expuestas características que distingan a los pacientes afectados por los dos microorganismos tan estrechamente relacionados.A revision of mycobacterial disease due to M simiae (n = 4 and "M. sherrisii" (n = 6 identified during an eight-year period is presented. Cases occurred among patients with AIDS (n = 6, previous history of silicosis (n = 2 or tuberculosis (n = 2. One case was lost to follow-up and the remaining nine responded poorly to chemotherapy based on clarithromycin, ethambutol and fluoroquinolones. Five patients died of whom four were HIV-positive, three remained chronic and one was cured. These microorganisms originated 2.1% of mycobacterioses cases detected in an eight-year period. Timely identification of this group of uncommon mycobacteria by molecular methods seems to be clinically relevant in order to warn of difficulties inherent to the treatment. However, the distinction between both closely related microorganisms might not be crucial for case

Outbreak of persistent cutaneous abscesses due to Mycobacterium chelonae after mesotherapy sessions, Lima, Peru Surto de abscessos cutâneos persistentes por Mycobacterium chelonae pós-mesoterapia, Lima, Peru

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César V Munayco

2008-02-01

Full Text Available Outbreaks of rapidly growing mycobacteria have been occasionally described. The article reports an outbreak of cutaneous abscesses due to Mycobacterium chelonae following mesotherapy in Lima, Peru. From December 2004 through January 2005, 35 subjects who had participated in mesotherapy training sessions presented with persistent cutaneous abscesses. Thirteen (37% of these suspected cases consented to underwent clinical examination. Skin punch-biopsies were collected from suspicious lesions and substances injected during mesotherapy were analyzed. Suspected cases were mainly young women and lesions included subcutaneous nodules, abscesses and ulcers. Mycobacterium chelonae was isolated from four patients and from a procaine vial. In conclusion, it is important to consider mesotherapy as a potential source of rapidly growing mycobacteria infections.Surtos de micobactérias de crescimento rápido têm sido relatados ocasionalmente. O estudo relata um surto de abscessos cutâneos por Mycobacterium chelonae após sessões de mesoterapia em Lima, Peru. De dezembro de 2004 a janeiro de 2005, 35 pessoas que haviam passado por sessões de mesoterapia apresentaram esses abscessos cutâneos. Treze (37% desses casos suspeitos concordaram em realizar exames clínicos. Foram realizadas biópsias de punção de pele de lesões suspeitas e examinadas substâncias injetadas durante a mesoterapia. Os casos suspeitos eram predominantemente mulheres jovens e as lesões incluíram nódulos subcutâneos, abscessos e úlceras. Mycobacterium chelonae foi isolada de quatro pacientes e de um frasco de procaína. Em conclusão, é importante considerar a mesoterapia como fonte potencial de infecções de micobactérias de crescimento rápido.

Lesão aguda esôfago - gástrica causada por agente químico Chemically induced esophagogastric acute injury

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P. R. Corsi

2000-06-01

Full Text Available O tratamento da ingestão de agentes químicos corrosivos continua controverso. A incidência desses episódios tem aumentado nas últimas décadas por várias razões. OBJETIVO: Analisar a ocorrência, as complicações e os resultados do tratamento da lesão esôfago - gástrica causada por agentes químicos. MÉTODOS: Foram estudados retrospectivamente 21 pacientes adultos com lesão esôfago-gástrica, causada por ingestão de substância química, admitidos até 23 dias após o episódio, no Serviço de Emergência da Santa Casa de Misericórdia de São Paulo num período de 12 anos. A média de idade foi 32,1 anos e 11 doentes pertenciam ao sexo feminino, as quais mais freqüentemente tinham intenções suicidas. A soda cáustica foi o produto mais ingerido (76,2%, ingestão de ácido muriático ocorreu em três casos (14,3%, amoníaco e ácido sulfúrico em um caso (4,8% cada. RESULTADOS: As lesões faríngeas e laríngeas estiveram freqüentemente associadas às lesões de esôfago, presentes em 18 casos (85,7%. As lesões esofágicas, gástricas e duodenais foram avaliadas e classificadas por endoscopia. Lesões graves esofágicas ou gástricas estiveram presentes em cinco casos cada. CONCLUSÃO: O tratamento e os resultados foram variados, mas sugeriram que a sondagem esofágica foi prejudicial. A mortalidade global foi 28,6%, mais elevada na lesão esofágica grau 3.Treatment of chemical agent ingestion remain controversial. The incidence of these episodes has increased over the several last decades due to a variety reasons. PURPOSE: To analise the occurance, complications and results of the treatment of chemically induced esophagogastric injury. METHODS: Twenty-one adult patients with chemically induced esophagogastric injury were retrospectively studied. The patients were admitted up to 23 days after ingestion of a chemical agent to the Emergency Department of Santa Casa of Sao Paulo University Hospital from August, in a 12-year

Efectividad de Cuatro Fungicidas en el Control de "La Quemazón de las Hojas" en Cebolla (Allium Cepa, Causada por Altemaria Porri

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Quintero Serpa Lumar

1960-06-01

Full Text Available En el presente trabajo, se considera la efectividad de cuatro fungicidas comerciales, bajo condiciones de campo, en el control de "la quemazón de las hojas" en cebolla, enfermedad causada por el Alternaria porri. Considerando como base para comprar la efectividad de los fungicidas el peso de los bulbos cosechados en cada tratamiento, se concluyó que el empleo de fungicidas aumentaba el rendimiento de las cosechas. El orden de efectividad de los cuatro fungicidas en el presente trabajo fue: Manzate, Parzate, Dithane Z-78 y Oxicob. Las conclusiones del presente trabajo deben considerarse como una contribución a este tema y solo a manera de trabajo preliminar; pero el hecho de haberse presentado diferencias considerables entre la producción de las parcelas tratadas y la testigo, sugiere la necesidad de experimentar más al respecto, con el fin de reforzar estos primeros resultados y averiguar sobre la efectividad de otros productos.

Micetoma por Nocardia brasiliensis: reporte de caso

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Miriam Guevara R

2003-07-01

Full Text Available Se presenta el caso de un paciente peruano, agricultor, con una infección cutánea de origen traumático causada por Nocardia brasiliensis, que evolucionó hacia la amputación del miembro inferior afectado. El diagnóstico se realizó por examen directo y cultivo del espécimen.

Mastite micótica em ruminantes causada por leveduras Mycotic mastitis in ruminants caused by yeasts

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Andréia Spanamberg

2009-02-01

Full Text Available A ocorrência de casos esporádicos de mastite causados por microrganismos de origem ambiental pode ser considerada como emergente e as leveduras, os fungos leveduriformes e os filamentosos são alguns dos principais agentes envolvidos. Em relação à mastite micótica, as leveduras são freqüentemente causas de infecções da glândula mamária em animais produtores de leite. A maior parte dos casos ocorre sob a forma de surtos localizados e/ou após tratamento com antimicrobianos. Os principais gêneros envolvidos são Candida e Cryptococcus, além de outros como Geotrichum, Pichia e Trichosporon. O objetivo do trabalho é revisar os aspectos gerais sobre a mastite micótica causada por leveduras e fungos leveduriformes, tais como etiologia, causas predisponentes, patogenicidade, diagnóstico, tratamento e profilaxia por meio de uma abordagem cronológica dos primeiros relatos, mas com enfoque principal nos resultados descritos recentemente na literatura veterinária nacional e internacional. O controle da mastite micótica é fundamentado em métodos preventivos, como adequado manejo da ordenha, correta higienização dos equipamentos e das instalações, com a finalidade de reduzir o número de animais acometidos, além de garantir a qualidade e a inocuidade dos produtos lácteos.The veterinary literature registers sporadic cases of infections caused by environmental microorganisms in which the main agents involved are yeasts, yeasts-like and filamentous fungi. In relation to dairy animals, the yeasts are more frequently incriminated as the aetiological agents of mycotic mastitis. Most cases occur under the form of localized outbreaks and / or after treatment with antimicrobial agents. The main genera involved in mastitis are Candida and Cryptococcus, besides others such as Geotrichum, Pichia and Trichosporon. The purpose of this review is to point out the main aspects of mastitis caused by yeasts and yeast-like fungi, such as etiology

Mastitis bovina causada por Staphylococcus coagulasa negativos

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Bonetto, César C.

2014-01-01

El presente Trabajo de Tesis se desprende como una línea de investigación del grupo de la UNRC dedicado al estudio de la problemática de la mastitis bovina tanto en aspectos básicos como aplicados con estudios genéticos, epidemiológicos y de virulencia de los principales microorganismos involucrados en la mastitis bovina. Staphylococccus coagulasa negativo (SCN) es un grupo bacteriano formado por varias especies que por lo general eran considerados flora oportunista de la piel y age...

Cytomegalovirus and other herpesviruses infections in heart and bone marrow transplant recipients Infecções causadas por citomegalovírus e outros vírus do grupo herpes em transplantados cardíacos e de medula óssea

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Adriana Weinberg

1990-10-01

Full Text Available From January 1988 to January 1989 all the heart transplant and bone marrow recipients at the Instituto do Coração of the Hospital das Clínicas of the University of São Paulo Medical School were studied for the incidence and morbidity associated with herpesviruses infections after transplantation. Five bone marrow and 5 heart transplant recipients were followed for a mean of 4.2 months post-transplantation. All the patients were seropositive for cytomegalovirus (CMV before admission and 80% experienced one or more recurrences during the observation period. Of the 12 episodes of CMV infection, that were identified in this study, 83% were accompanied by clinical or laboratory abnormalities. However, there was only one case of severe disease. The overall incidence of infection for herpes simplex (HSV was 50%. Although most of HSV reactivations were oral or genital, one case of HSV hepatitis occurred. One of the 6 episodes of HSV infections that were treated with acyclovir showed an unsatisfactory response and was successfully managed with ganciclovir. All the individuals had anti-varicella zoster virus antibodies, but none of them developed infection. The study emphasizes the importance of active diagnostic surveillance of herpesvirus infections in transplant patients. Both CMV and HSV reactivations showed high incidence and important morbidity and thus, deserve prophylactic therapy.De janeiro de 1988 a janeiro de 1989 todos os pacientes submetidos a transplante de coração ou de medula óssea no Instituto do Coração do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo foram estudados quanto à incidência e morbidade das infecções pós-transplante causadas por vírus do grupo herpes. Cinco recipientes de medula óssea e 5 transplantados cardíacos foram observados por um período médio de 4.2 meses após o transplante. Todos os pacientes tinham sorologia positiva para citomegalovírus (CMV antes do transplante

ALTERAÇÕES MICROMETEOROLÓGICAS CAUSADAS POR TÚNEIS BAIXOS DE TELA PLÁSTICA PRETA CULTIVADOS COM ALFACE

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Galileo Adeli Buriot

1994-01-01

Full Text Available RESUMO Foram determinadas alterações micrometeorológicas causadas por tela plástica preta com 18, 30 e 50% de atenuação da radiação solar incidente, durante os meses de verão, em Santa Maria, RS. O experimento foi conduzido no Campo Experimental do Departamento de Fitotecnia da UFSM, onde foram cultivados quatro canteiros com alface, três deles cobertos com túneis de tela plástica. O estudo foi realizado em 3 períodos: de 30/11/90 a 17/01/91, de 06/02/91 a 25/03/91 e de 10/12/91 a 01/03/92. Mediu-se a radiação solar global, radiação solar na faixa de comprimento de onda do infravermelho e a luminosidade, temperatura diurna do solo, temperatura mínima e diurna do ar e umidade do solo e do ar. Os resultados mostraram que a temperatura do solo e do ar e a perda de água do solo são menores sob os túneis do que no exterior. As diferenças cresceram com o aumento da atenuação e a temperatura mínima do ar e a umidade relativa do ar são semelhantes no interior e exterior dos túneis e entre os túneis com diferentes percentagens de atenuação.

Higiene alimentaria para la prevención de trastornos digestivos infecciosos y por toxinas

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G. Manuel Moreno, Dr.

2010-09-01

Full Text Available El principal factor que interviene en el origen y prevención de las enfermedades trasmitidas por los alimentos es la higiene alimentaria. Dichas enfermedades son causadas por la ingestión de alimentos o agua contaminados con microorganismos patógenos ocasionando una infección o por la ingestión de alimentos contaminados con toxinas. Los principales agentes involucrados son Escherichia Coli, Campylobacter, Salmonella, Shigella, Listeria Monocytogenes, Norovirus, virus Hepatitis A, Astrovirus, Rotavirus, y Virus Coxsackie. Toxinas producidas por hongos o por microflora marina y los contaminantes orgánicos persistentes pueden también causar serios problemas de salud. La inocuidad alimentaría ha tomado relevancia debido a una mayor exigencia por consumidores cada día más informados y por las demandas del comercio exterior. Medidas que aseguren una adecuada higiene alimentaría nos permitirá prevenir enfermedades, principalmente digestivas, causadas por variados agentes en los alimentos. Esto se logra por la implementación de las medidas propuestas por la Comisión Internacional conocida como Codex Alimentarius.

Meningite tuberculosa: avaliação da reação em cadeia da polimerase (PCR como ferramenta diagnóstica – um estudo piloto

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Guilherme Geib

2008-09-01

Full Text Available Meningite é uma forma grave e potencialmente fatal de tuberculose. O diagnóstico envolve a detecção de bacilos álcool-ácido resistentes no líquido cefalorraquidiano por microscopia ou cultura. Entretanto, a dificuldade de detectar o organismo representa um desafio ao diagnóstico. O uso da reação em cadeia da polimerase (PCR na abordagem diagnóstica de meningite causada por Mycobacterium tuberculosis (MTB tem sido relatado como um método rápido e preciso, com diversos kits comerciais disponíveis. Como alternativa, algumas instituições vêm desenvolvendo testes in house com baixo custo. Em nossa instituição, usamos PCR in house para tuberculose. O desempenho de nossa PCR para o diagnóstico de meningite causada por MTB foi analisado em 148 pacientes consecutivos, usando a cultura do MBT como padrão-ouro. A sensibilidade da PCR no líquido cefalorraquidiano para o diagnóstico de meningite causada por MTB foi de 50%, especificidade de 98,6% e concordância coma cultura de 96% (kappa = 0,52. O desempenho de nossa PCR é semelhante ao obtido com os kits comerciais disponíveis.

Interação das lesões causadas pela sarna-da-macieira (Venturia inaequalis na capacidade de infestação dos frutos por lagartas de Grapholita molesta (Lepidoptera: Tortricidae

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Oscar Arnaldo Batista Neto e Silva

2011-12-01

Full Text Available Em monitoramentos de pragas realizados na cultura da macieira foram observadas maiores infestações da mariposa oriental (Grapholita molesta em frutos provenientes de pomares com maior incidência de lesões causadas pela sarna-da-macieira (Venturia inaequalis. Para validar esta observação, conduziu-se um experimento em laboratório com o objetivo de verificar a influência de lesões da sarna da macieira em frutos, na capacidade de infestação por G. molesta. Foram utilizados frutos (n=200 de macieira da variedade Gala com sintomas da sarna (n=100 e frutos sadios (n=100. Uma lagarta recém-eclodida foi inoculada em cada fruto e a avaliação foi realizada 10 dias após a infestação, determinando-se o número de lagartas que conseguiram penetrar nos frutos. Houve diferença significativa na capacidade de penetração das lagartas associado a presença de lesões da sarna (87% quando comparado com frutos sadios (61%. Conclui-se que frutos de maçã da cv. Gala atacados por Venturia inaequalis são mais infestados por lagartas de primeiro ínstar de Grapholita molesta.

Compactação do solo causada por tratores florestais na colheita de Pinus taeda L. na região sudoeste do Paraná

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Denise Andréia Szymczak

2014-08-01

Full Text Available A compactação vem-se tornando um dos principais impactos causados ao solo pelas atividades de colheita florestal mecanizada. O peso e movimentação das máquinas no momento do corte e extração da madeira, aliados à condição de umidade do solo imprópria para tal, são as principais causas da degradação estrutural do solo, verificada, principalmente, por alterações em suas propriedades físicas. Nesse sentido, este trabalho visou identificar a compactação causada ao solo devido às operações de colheita florestal de Pinus taeda L. realizada em três diferentes níveis de umidade do terreno. O estudo foi realizado em área de uma empresa florestal localizada no Paraná sobre Latossolo Vermelho. Os tratamentos foram compostos pela interação de três fatores, sendo eles: umidade (colheita em dia chuva, três e sete dias após a chuva, operações de colheita (com passagem de máquinas, sem passagem/eventual queda de árvores e estaleiro, e a ocasião de coleta (antes e depois da colheita. A biomassa residual da colheita florestal foi essencial para minimizar a compactação do solo causada pelo tráfego das máquinas que foi próxima da máxima. A gradação de umidade representada pela colheita em diferentes números de dias após uma chuva não influenciou, de maneira diferenciada, a compactação do solo. Entretanto, as operações de colheita impactaram o solo até a profundidade de 10 cm, causando a compactação nas linhas de tráfego das máquinas, enquanto as demais operações não provocaram alterações na densidade, macroporosidade e resistência à penetração.

Indicadores de riesgo de morbilidad prevenible causada por medicamentos Risk indicators of preventable morbidity related to drug utilization

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Ana Dago Martínez

2007-02-01

Full Text Available Objetivos: Seleccionar y estudiar la aceptabilidad, en términos de relevancia y pertinencia, de ciertas situaciones clínicas que puedan usarse como indicadores de riesgo de morbilidad prevenible causada por medicamentos y que sean utilizables en las farmacias comunitarias. Método: Método Delphi, en 2 rondas, con un panel de 14 expertos médicos y farmacéuticos que valoraron la relevancia y pertinencia de 68 tipos de situaciones clínicas como indicadores de morbilidad potencial relacionada con medicamentos, detectable por el profesional en su medio, con evidencia científica de resultado adverso previsible, frecuentes en el medio ambulatorio y con causa y resultado controlables. Resultados: Se consideraron utilizables y pertinentes 43 de los 68 indicadores estudiados, que se referían a 3 ámbitos: tipo de medicamento (medicamentos de estrecho margen terapéutico, con dosis individualizada y con reacciones adversas frecuentes y graves, problema de salud (problemas crónicos, especialmente asma, enfermedad cardíaca, tiroidea, prostática y dolor y tipo de paciente (ancianos y/o polimedicados. Los farmacéuticos sobrevaloraron sistemáticamente ciertos indicadores en relación con los médicos, aunque las diferencias no fueron significativas. Conclusiones: Se identificaron 43 indicadores de morbilidad potencial relacionada con los medicamentos e identificables por los profesionales en su entorno.Objective: To select clinical situations that can be used as risk indicators of preventable morbidity caused by drugs at the community pharmacies, and to study their acceptability, in terms of pertinence and relevance. Methods: We used the Delphi technique, in 2 rounds, by a panel of 14 medical doctors and pharmacists experts, to study the relevance of 68 types of clinical situations as risk indicators of preventable morbidity related to drug utilization used by health professionals in community pharmacies, with scientific evidence of foreseeable

Úlceras por presión causadas por dispositivos clínicos

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Iglesias Ruisánchez, Sandra

2017-01-01

Las úlceras por presión (UPP) son lesiones comunes en los pacientes hospitalizados, especialmente en las unidades de cuidados intensivos (UCI). Los enfermos críticos tienen un riesgo elevado de desarrollar UPP, debido principalmente a la limitación de la movilidad, disminuyendo su capacidad para cambiar activamente su posición en la cama o asiento. Además, el efecto de fármacos anestésicos y sedantes, puede causar una pérdida de la percepción sensorial cutánea. Los diferentes dispositivos clí...

HLA in Brazilian Ashkenazic Jews with chronic dermatophytosis caused by Trichophyton rubrum Antígenos Leucocitários Humanos (HLA em Judeus Ashkenazitas Brasileiros portadores de dermatofitose crônica causada por Trichophyton rubrum

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Aya Sadahiro

2004-06-01

Full Text Available The frequency of HLA (Human Leucocyte Antigens was analyzed in 25 non-consanguineous Brazilian Ashkenazic Jews, resident in the city of São Paulo, Brazil, suffering from chronic dermatophytosis caused by T. rubrum, and in 25 non-infected individuals belonging to the same ethnic group. Statistically significant values (pA freqüência dos HLA foi analisada em 25 Judeus Ashkenazitas, não consangüíneos, residentes em São Paulo, Brasil, com dermatofitose crônica causada por T. rubrum e em 25 indivíduos sadios, pertencentes ao mesmo grupo étnico dos pacientes. Observou-se valor estatisticamente significante (p<0,05 para HLA-B14 associado a resistência à dermatofitose crônica enquanto HLA-DQB1*06 (p=0,05 possivelmente relacionado a susceptibilidade. Estes achados indicam que o desenvolvimento da dermatofitose crônica pode ser influenciado por genes localizados no cromossomo 6, na região do complexo principal de histocompatibilidade.

Rotura neumática de colon por aire comprimido

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VITÓN H,REBECA; QUADROS B,PEDRO; RODRÍGUEZ M,JOSÉ ANTONIO

2010-01-01

La ruptura del colon causada por aire comprimido a alta presión es rara. A propósito de un caso se revisan los mecanismos de producción, tipos de roturas, clínica, diagnóstico y tratamiento de estos traumatismos.

Papel dos medicamentos nas intoxicações causadas por agentes químicos em município da Bahia, no período de 2007 a 2010

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Amanda dos Santos Teles

2013-04-01

Full Text Available A intoxicação medicamentosa é um problema de grande relevância para a saúde pública. No Brasil, os medicamentos são os principais agentes responsáveis por intoxicações humanas. Este artigo objetivou descrever o perfil e a evolução das intoxicações no município de Feira de Santana, Bahia, nos anos 2007 a 2010, e analisar a morbimortalidade causada por medicamentos. Neste estudo transversal, com caráter descritivo, foram analisados 631 casos registrados no SINAN. As variáveis utilizadas foram: sexo; faixa etária; raça; escolaridade; ocupação; evolução do caso notificado; zona de ocorrência da intoxicação; circunstância determinante para a ocorrência da intoxicação; tipo de exposição; frequência deste agravo; e tipo de unidade notificadora. Constatou-se que a intoxicação por medicamentos representou 33% das intoxicações, seguida de “raticidas” (18%, sendo a “tentativa de suicídio”, circunstância responsável, em 2010, por 81% das notificações. O sexo ‘feminino’ apresentou o maior número de casos registrados, bem como as crianças de 1 a 4 anos e os adultos jovens. A letalidade observada em Feira de Santana foi muito maior do que a média regional e nacional, excetuando-se o ano de 2009. A Vigilância em Saúde deve priorizar ações educativas, preventivas e intersetoriais, que assegurem o uso correto de medicamentos e melhorem seu impacto na saúde, bem como investir na sensibilização continuada dos profissionais da Atenção Básica para melhorar a notificação deste importante agravo.

In vitro susceptibility of Mycobacterium tuberculosis, Mycobacterium africanum, Mycobacterium bovis, Mycobacterium avium, Mycobacterium fortuitum, and Mycobacterium chelonae to ticarcillin in combination with clavulanic acid.

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Casal, M J; Rodriguez, F C; Luna, M D; Benavente, M C

1987-01-01

The in vitro susceptibility of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium avium, Mycobacterium fortuitum, and Mycobacterium chelonae (M. chelonei) to ticarcillin in combination with calvulanic acid (CA) was studied by the agar dilution method. All the M. tuberculosis, M. bovis, and M. africanum strains were inhibited at a ticarcillin concentration of 32 micrograms/ml or lower in combination with 5 micrograms of CA. M. chelonae and M. avium strains ...

El estado de las lesiones causadas por el tránsito en México: evidencias para fortalecer la estrategia mexicana de seguridad vial

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Ricardo Pérez-Núñez

2014-05-01

Full Text Available Mediante la revisión de información secundaria oficial, publicaciones científicas y el análisis de la ENSANut-2012, este trabajo busca proveer una visión integral de la magnitud actual de las lesiones causadas por el tránsito (LCT en México y analizar críticamente la respuesta social organizada. Las altas tasas de choques, lesiones, muertes y discapacidad atribuidas a este problema de salud pública, representan un elevado costo para la sociedad mexicana, en especial para los hogares. Se discute cómo la respuesta mexicana se ha enfocado a los automovilistas, dejando fuera a usuarios vulnerables de la vía pública y se ha privilegiado la implementación de estrategias poco efectivas. A pesar de que se dispone de legislación para los principales factores de riesgo, se ha descuidado su correcta aplicación. La estrategia mexicana podría fortalecerse implementando estrategias de movilidad seguras, equitativas, saludables y sostenibles para los diferentes actores de la vía pública. A pesar de los avances que se han dado en términos de prevención de LCT, existen aún retos y oportunidades para el país.

Osteomalacia inducida por tumor: hemangiopericitoma rinosinusal

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Enriqueta M. Serafini

2013-02-01

Full Text Available La osteomalacia inducida por tumor es una rara enfermedad del metabolismo óseo caracterizada por el aumento en la excreción de fosfato a nivel renal seguido de hipofosfatemia. Es causada por agentes fosfatúricos producidos por determinados tumores. La resección total del tumor resulta en la completa reversión de las anormalidades bioquímicas, la desaparición de las manifestaciones clínicas y los hallazgos en los estudios por imágenes. Presentamos el caso de un varón de 61 años con cuadro clínico y laboratorio compatibles con osteomalacia oncogénica inducida por tumor mesenquimático de localización rinosinusal. En nuestro caso el diagnóstico histológico correspondió a una neoplasia de tipo vascular: hemangiopericitoma.

Fatores de risco associados à mastite bovina causada por Prototheca zopfii Risk factors associated with bovine mastitis caused by Prototheca zopfii

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Aline Artioli Machado Yamamura

2008-06-01

Full Text Available Este trabalho teve como objetivo o estudo de fatores de risco associados à mastite bovina causada por Prototheca zopfii. Foram analisadas 13 propriedades leiteiras dos Estados do Paraná e de São Paulo, segundo os seguintes critérios de seleção: confirmação prévia de casos de mastite por Prototheca spp., triagem pela pesquisa de Prototheca spp. em tanques de expansão e latões e rebanhos com contagem de células somáticas acima de 5x105cel mL-1. As amostras coletadas consistiram de: leite, água, solo, fezes e swab de teteiras. Prototheca spp. foi isolada de amostras de leite dos quartos mamários com mastite clínica ou subclínica em uma propriedade e de amostras de leite e do ambiente em quatro propriedades, nas quais foi isolada em amostras de: água de bebedouro, abastecimento, esgoto, empoçada no piso de estábulo e sala de ordenha, solo de piquete e pasto, teteiras, fezes de bezerros e suínos. Do total de 383 vacas examinadas, Prototheca spp. foi isolada em 20 (5,2% vacas, sendo caracterizada como P. zopfii em 18. Os fatores de risco associados à mastite causada por P. zopfii foram: criação das vacas a pasto, alimentação dos animais com pasto e silagem, realização de ordenha mecânica em estábulo, permanência das vacas após ordenha em piquete sem alimento, criação de suínos próxima às instalações dos bovinos, existência de cães, gatos e roedores, falta de higienização dos tetos com água, pré-imersão dos tetos em aplicador com retorno e sem a troca do anti-séptico, alimentação dos bezerros com leite de vacas com mastite clínica e serem as vacas da raça holandesa.This research had as objective the study of risk factors associated with bovine mastitis caused by Prototheca zopfii. Thirteen dairy herds in Paraná and São Paulo states were analyzed and selected according to the following criteria: previous confirmation of Prototheca spp. mastitis cases, screening of Prototheca spp. in bulk tanks and

Soft tissue abscess and lymphadenitis due to Mycobacterium avium Complex as an expression of immune reconstitution inflammatory syndrome after a second scheme of highly active antiretroviral therapy Linfadenitis y absceso subcutáneo por Complejo Mycobacterium avium como manifestación de síndrome inflamatorio de reconstitución inmune luego de un segundo esquema de terapia antirretroviral de gran actividad

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Marcelo Corti

2007-08-01

Full Text Available Immune reconstitution inflammatory syndrome (IRIS is an atypical and unexpected reaction related to highly active antiretroviral therapy (HAART in human immunodeficiency virus (HIV infected patients. IRIS includes an atypical response to an opportunistic pathogen (generally Mycobacterium tuberculosis, Mycobacterium avium complex, cytomegalovirus and herpes varicella-zoster, in patients responding to HAART with a reduction of plasma viral load and evidence of immune restoration based on increase of CD4+ T-cell count. We reported a case of a patient with AIDS which, after a first failure of HAART, developed a subcutaneous abscess and supraclavicular lymphadenitis as an expression of IRIS due to Mycobacterium avium complex after starting a second scheme of HAART.El síndrome inflamatorio de reconstitución inmune (SIRI es una reacción atípica e inesperada relacionada con el tratamiento antirretroviral de gran actividad (TARGA en pacientes infectados por el virus de la inmunodeficiencia humana (VIH. El SIRI representa una respuesta inflamatoria frente a un patógeno oportunista (generalmente Mycobacterium tuberculosis, Complejo Mycobacterium avium, citomegalovirus y herpes varicela-zóster en pacientes que responden a la TARGA con una marcada reducción de la carga viral en plasma y evidencia de una recuperación inmunológica expresada por el incremento de los niveles de linfocitos T CD4+. Presentamos el caso de un paciente con síndrome de inmunodeficiencia adquirida que desarrolló un absceso subcutáneo en muslo derecho y una adenitis supraclavicular izquierda como manifestación de SIRI por Complejo Mycobacterium avium luego del inicio de un segundo esquema de TARGA.

Conidiobolomicose causada por Conidiobolus lamprauges em ovinos no Estado de Santa Catarina Conidiobolomycosis caused by Conidiobolus lamprauges in sheep in the state of Santa Catarina, Brazil

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Fernando Henrique Furlan

2010-07-01

Full Text Available Descreve-se um surto de conidiobolomicose em ovinos no Estado de Santa Catarina. O surto ocorreu entre os meses de dezembro e março de 2006, no município de Santo Amaro da Imperatriz, região litorânea do Estado. A propriedade possuía 75 ovinos da raça Santa Inês e seis desses animais adoeceram. Clinicamente os animais doentes apresentavam dificuldade respiratória, corrimento nasal seroso a mucossanguinolento e, por vezes exolftalmia. Na necropsia verificou-se uma massa amarelada na região etmoidal e adjacências que, às vezes, atingia os linfonodos regionais, cérebro, globo ocular e pleura. Microscopicamente a massa caracterizava-se por infiltrado inflamatório granulomatoso com áreas necróticas associadas a hifas largas pouco ramificadas. Através de exame molecular detectou-se DNA de Conidiobolus lamprauges. Os aspectos clínicos, epidemiológicos, macroscópicos, microscópicos e moleculares caracterizam a conidiobolomicose causada por Conidiobolus lamprauges em ovinos.An outbreak of conidiobolomycosis affecting sheep in the State of Santa Catarina, Southern Brazil is reported. The disease occurred in six Santa Inês breed sheep from a flock of 75 during the rainy season. Common clinical signs were noisy respiration and dyspnea, serous to mucosanguineous nasal discharge and exophthalmus. At necropsy there was a dense yellow mass in the nasopharyngeal area affecting the ethmoidal region, turbinate bones and occasionally limph nodes, central nervous system and pleura. Histopathologycally there was multifocal granulomas whith an eosinophilic necrotic reaction containing ribbon type hyphae similar to zygomycetous fungi. At molecular examination Conidiobolus lamprauges DNA was detected. The clinical, epidemiological, macroscopical, microscopical and molecular aspects characterize conidiobolomycosis caused by Conidiobolus lamprauges in sheep.

Aspectos epidemiológicos de pacientes com lesões ungueais e cutâneas causadas por Scytalidium spp Epidemiological aspects of patients with ungual and cutaneous lesions caused by Scytalidium spp

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Ana Paula Martins Xavier

2010-12-01

Full Text Available FUNDAMENTO: As dermatomicoses causadas por fungos filamentosos não dermatófitos são infecções raras, exceto as onicomicoses, cuja prevalência vem crescendo nos últimos anos. Dentre esses agentes etiológicos destacam-se o Scytalidium dimidiatum e o S. hyalinum, fungos emergentes responsáveis por micoses em unhas e pele. OBJETIVO: Investigar as características epidemiológicas das onicomicoses e micoses de outras localizações causadas pelos fungos do gênero Scytalidium, utilizando-se como parâmetros sexo, idade e localizações das lesões. MÉTODOS: Avaliaram-se 81 amostras com cultura positiva para o gênero em estudo, oriundas de 74 pacientes encaminhados ao Laboratório de Investigação em Dermatologia (ID situado na cidade do Rio de Janeiro (RJ, no período de 1997 a 2006. As amostras foram submetidas a confirmação diagnóstica por exame direto e cultura. RESULTADOS: A prevalência de onicomicoses por Scytalidium spp. foi de 0,87%, entre as idades de 41 e 60 anos (48,64%. Em relação à localização das lesões, os pés foram mais acometidos (91,36%, com predomínio do hálux esquerdo. No exame direto, as estruturas mais encontradas foram hifas hialinas; na cultura, a espécie S. dimidiatum foi a mais frequente. CONCLUSÃO: As onicomicoses por Scytalidium spp. são raras e o S. dimidiatum foi a espécie mais isolada neste laboratório no período em estudo.BACKGROUND: Dermatomycoses caused by non-dermatophyte filamentous fungi are rare infections, except for onychomycosis, whose prevalence has increased over the past few years. Among these etiologic agents, we highlight Scytalidium dimidiatum and S. hyalinum, emergent fungi that cause mycoses that affect the nails and skin. OBJECTIVE: To investigate the characteristics of onychomycosis and other mycoses caused by the fungi Scytalidium spp, using sex, age and site of infection as parameters. METHODS: Eighty-one samples were evaluated showing positive culture for Scytalidium

Lesiones en el hombro causadas por electrocución

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Herrero Barcos, L.; Martínez Martín, Angel Antonio; Herrera Rodríguez, Antonio; Cuenca Espiérrez, Jorge; Panisello Sebastiá, Juan José

2001-01-01

Las lesiones en el hombro tras sufrir una descarga eléctrica son raras. Presentamos siete pacientes con luxaciones y fracturas en el hombro debidas a una electrocución. Un alto porcentaje de estas lesiones se diagnostican tarde, empeorando de esta manera el pronóstico. Por ello, ante una electrocución, debemos pensar en una lesión de hombro. Shoulder injuries after an electrical shock are rare. We report seven cases with dislocation and fractures of the shoulder due to electrocution. A hig...

Aislamiento y caracterización de proteínas de Mycobacterium tuberculosis H37Rv con capacidad de modular la apoptosis de los macrófagos

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Blanca Ortiz

2001-04-01

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En los últimos años se ha descrito que la inducción de apoptosis puede ser un mecanismo de defensa contra las infecciones intracelulares, ya que se altera el microambiente intracelular, perdiéndose la permisividad para el crecimiento de microorganismos invasores. En el modelo murino la infección causada por la Mycobacterium tuberculosis induce apoptosis dependiendo de un delicado balance entre factores anti vs pro-apoptóticos, tanto del macrófago hospedero como de la
micobacteria. La apoptosis depende de productos derivados de la micobacteria viable como el PPD, mientras que antígenos estructurales como el ManLAM pueden inhibirla. Una mayor caracterización de los antígenos micobacterianos que modulan la apoptosis es importante para entender la fisiopatología de la enfermedad y desarrollar estrategias novedosas para su control. En este trabajo se tiene como objetivo purificar antígenos
micobacterianos y evaluar su papel en la modulación de la apoptosis de macrófagos murinos.

Mycobacterium chelonae y Mycobacterium abscessus: patógenos emergentes

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Mónica M. Ortegón

1996-09-01

Full Text Available Mycobacterium chelonae es el nombre correcto para la micobacteria aislada en 1903 de los pulmones enfermos de una tortuga marina. En una especie distinta de Mycobacterium fo/tuitum, aislado de ranas en 1905, y de Mycobacterium abscessus, considerado actualmente como una subespecie de M chelonae. Estas tres especies son las únicas patógenas para el hombre dentro del grupo de micobacterias ambientales o atipicas, de crecimiento rápido, las cuales se caracterizan por formar colonias en cultivo en menos de siete días. Son agentes etiológicos de nódulos y abscesos cutáneos, localizados y diseminados, de lesiones postoperatorias, usualmente en la cicatriz quirúrgica, de lesiones pulmonares y de linfadenitis granulomatosa, de osteomielitis y de queratitis, entre otras. Las lesiones cutáneas y de los tejidos blandos son las más frecuentes y resultan generalmente de la inoculación traumática de esta micobacteria. Histopatológicamente, los nódulos y abscesos muestran un proceso inflamatorio, supurativo y granulomatoso, mixto, en el que en la cuarta parte de los casos pueden demostrarse conglomerados de bacilos ácido alcohol resistentes, que tienden a estar situados en una vacuola en el centro del absceso. En Colombia, se han descrito tres brotes de abscesos subcutáneos producidos por bacterias ambientales, secundarios a la aplicación de inyecciones contaminadas con el germen causal: en 1981, en Bucaramanga, luego de la aplicación de la vacuna contra la fiebre amarilla, en 50 personas, la mayoría niños; en 1989, en Medellin, por la inyección subcutánea de alergenos, en 13 personas; y, en 1993, en varias ciudades de la costa atlántica, luego de aplicaciones subcutáneas de xilocaína, como tratamiento bionergético, en 297 pacientes. Existen otros informes aislados de casos posttraumáticos.La enfermedad diseminada por micobacterias de rápido crecimiento, se presenta en pacientes inmunosuprimidos. En la biopsia, predominan los

Mastite micótica em ruminantes causada por leveduras

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Spanamberg,Andréia; Sanches,Edna Maria Cavallini; Santurio,Janio Morais; Ferreiro,Laerte

2009-01-01

A ocorrência de casos esporádicos de mastite causados por microrganismos de origem ambiental pode ser considerada como emergente e as leveduras, os fungos leveduriformes e os filamentosos são alguns dos principais agentes envolvidos. Em relação à mastite micótica, as leveduras são freqüentemente causas de infecções da glândula mamária em animais produtores de leite. A maior parte dos casos ocorre sob a forma de surtos localizados e/ou após tratamento com antimicrobianos. Os principais gêneros...

Síndrome hemolítico-urêmica causada por gencitabina em uma paciente jovem com colangiocarcinoma

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John Fredy Nieto-Ríos

2016-06-01

Full Text Available Resumo A gencitabina é um fármaco utilizado no tratamento de vários tipos de neoplasias malignas. Há poucas descrições de associação entre a droga e a síndrome hemolítico-urêmica (SHU, apesar de os pacientes em questão terem ido a óbito em pelo menos 50% dos casos. O presente artigo relata o caso de uma paciente com 25 anos de idade em remissão diagnosticada com colangiocarcinoma que apresentou anemia hemolítica microangiopática acompanhada de insuficiência renal aguda anúrica após cinco ciclos de quimioterapia com gencitabina; as manifestações eram condizentes com SHU causada pelos efeitos colaterais do medicamento. A administração de gencitabina foi interrompida, e a paciente foi tratada com hemodiálise, transfusões de sangue, trocas de plasma, corticosteroides, doxiciclina e rituximabe. Foi atingido um desfecho favorável; mais especificamente, a hemólise foi controlada e a função renal foi plenamente restabelecida.

Efecto del ácido acetil salicílico y Bacillus subtilis en la infección causada por Cucumber mosaic virus en calabacita

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E. Maldonado-Cruz

2008-01-01

Full Text Available Dos conocidos agentes inductores de resistencia en las plantas, ácido acetil salicílico y Bacillus subtilis, fueron evaluados con la finalidad de conocer su efecto en la infección causada por el Cucumber mosaic virus (CMV en Cucurbita pepo var. Zucchini grey.Se estableció un diseño experimental completamente al azar en invernadero con cinco tratamientos y ocho repeticiones: Bacillus subtilis aplicado al suelo, B. subtilis aplicado al follaje; B. subtilis aplicado al suelo y al follaje, ácido acetil salicílico aplicado al follaje y testigo (sin aplicación de ninguno de los productos antes indicados. Plantas de 20 días de edad fueron inoculadas mecánicamente con Cucumber mosaic virus y 15 días después de la inoculación se evaluaron las variables peso de biomasa fresca y concentración viral mediante la técnica de DAS-ELISA. Los resultados muestran que las plantas inoculadas con B. subtilis al suelo y ácido acetil salicílico tuvieron significativamente mayor peso de biomasa fresca comparados con el tratamiento Testigo (P¿0.05. Las plantas de todos los tratamientos donde se aplicó B. subtilis (al suelo, al follaje o al suelo y al follaje tuvieron una concentración viral significativamente menor comparadas con el testigo (P¿0.05 en la prueba de ELISA.

Mastite subclínica causada por Staphylococcus aureus: custo-benefício da antibioticoterapia de vacas em lactação Subclinical mastitis caused by Staphylococcus aureus: cost benefit analysis of antibiotic therapy in lactating cows

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L.F. Zafalon

2007-06-01

Full Text Available Avaliou-se a relação custo-benefício do tratamento da mastite subclínica bovina causada por Staphylococcus aureus. Foram selecionados 270 quartos mamários com mastite subclínica e sadios, divididos em quatro grupos de acordo com o estádio de lactação e o tratamento. O grupo 1 foi formado por animais entre 10 e 60 dias da lactação e tratados contra mastites; o grupo 2 incluiu animais entre 61 dias da lactação e dois meses antes da secagem e tratados contra mastite; o grupo 3 foi formado por animais entre 10 e 60 dias da lactação, não tratados contra mastite; e o grupo 4 foi formado por animais entre 61 dias em lactação e dois meses antes da secagem, não tratados. O tratamento foi realizado pela infusão intramamária de 150mg de gentamicina, uma vez ao dia. A reavaliação foi efetuada após 30 dias. Para os cálculos dos custos com o tratamento, foram considerados uma prevalência de S. aureus de 5% e os gastos com medicamento, descarte do leite, antibiograma e mão-de-obra. Observou-se redução de 2% e 14% das receitas nos grupos 1 e 2, respectivamente, quando comparada com as receitas obtidas antes do tratamento, demonstrando ser economicamente inviável o tratamento da mastite subclínica bovina causada por S. aureus, durante a lactação.Economic evaluation of the treatment bovine subclinical mastitis caused by S. aureus was evaluated. Two hundred and seventy udder quarters with or without subclinical mastitis were distributed into four groups, in conformity to lactational stage and treatments. Group 1 included animals treated between 10 and 60 days of lactation; group 2 included animals treated from 61 days of lactation to two months before drying; group 3 included animals no treated between 10 and 60 days of lactation; group 4 included animals no treated from 61 days of lactation to two months before drying. Treatment with gentamicin (150mg was accomplished by intramammary doses, once a day, after performing sensitivity

EN EL CENTENARIO DEL DESCUBRIMIENTO DEL MYCOBACTERIUM TUBERCULOSIS POR ROBERTO KOCH. 24 DE MARZO DE 1982

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Gilberto Rueda Pérez

1982-12-01

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Hace exactamente 100 años, señores académicos, Roberto Koch presentó en concisa, lacónica y podríamos decir perfecta comunicación, a la Sociedad de Fisiología de Berlín, la demostración de que la TBC tenía como causa única la proliferación en el organismo vivo, del germen denominado por él Mycobacterium Tuberculosis.

Esta comunicación que hoy conmemoramos, cambió el curso de la microbiología, y sentó las bases para el desarrollo posterior del tratamiento etiológico de las enfermedades infecciosas. Años más tarde, Koch recibiría el máximo galardón otorgado por Nobel a los grandes benefactores de la humanidad, por este descubrimiento. Sería glorificado por sus
contemporáneos y por quienes posteriormente hemos tenido el privilegio de ejercer la medicina en el mundo civilizado, así como por quienes padecieron y padecen aún el terrible mal, al ver cercana su curación gracias a los descubrimientos recientes que han permitido derrotar al más temible enemigo de la patología humana: el Bacilo puesto en evidencia
por este retraído sabio alemán.

Pero no todo fue gloria y renombre en la vida de Roberto Koch. Vale la pena rememorar a grandes rasgos en este Centenario y ante esta Academia de Medicina Colombiana, algunos de los hechos sobresalientes de la vida de este hombre excepcional, que le dio luz en su época y le sigue dando a la ciencia en general, y que sentó premisas que siguen vigentes en nuestros días a través del postulado que lleva su nombre, ineludibles para quienes quieren demostrar científicamente hechos bacteriológicos.

Nace el 11 de diciembre de 1843 en la pequeña población de Klausthal en Hanover, y allí transcurre su primera juventud provinciana y sencilla para luego viajar a Gotinga, en cuya universidad estudia medicina, destacándose siempre por su dedicación y método en las asignaturas, recibiendo su doctorado en 1886, a los 23 años, bajo la tutela del Profesor

Jejunal perforation caused by abdominal angiostrongyliasis Perfuração jejunal causada por angiostrongilíase abdominal

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Jaques WAISBERG

1999-09-01

Full Text Available The authors describe a case of abdominal angiostrongyliasis in an adult patient presenting acute abdominal pain caused by jejunal perforation. The case was unusual, as this affliction habitually involves the terminal ileum, appendix, cecum or ascending colon. The disease is caused by the nematode Angiostrongylus costaricensis, whose definitive hosts are forest rodents while snails and slugs are its intermediate hosts. Infection in humans is accidental and occurs via the ingestion of snail or slug mucoid secretions found on vegetables, or by direct contact with the mucus. Abdominal angiostrongyliasis is clinically characterized by prolonged fever, anorexia, abdominal pain in the right-lower quadrant, and peripheral blood eosinophilia. Although usually of a benign nature, its course may evolve to more complicated forms such as intestinal obstruction or perforation likely to require a surgical approach. Currently, no efficient medication for the treatment of abdominal angiostrongyliasis is known to be available. In this study, the authors provide a review on the subject, considering its etiopathogeny, clinical picture, diagnosis and treatment.Os autores descrevem caso de angiostrongilíase abdominal em doente adulto que se manifestou como abdômen agudo devido à perfuração de alça jejunal, evento raro, uma vez que esta afecção geralmente envolve o íleo terminal, apêndice, ceco ou cólon ascendente. A doença é causada pelo nematódeo Angiostrongylus costaricensis cujos hospedeiros definitivos são roedores silvestres e os hospedeiros intermediários são caracóis e caramujos. A infecção em humanos é acidental e ocorre pela ingestão de secreção mucóide destes invertebrados presentes em vegetais ou por contato direto com o muco. A angiostrongilíase abdominal é clinicamente caracterizada pela presença de febre prolongada, anorexia, dor no quadrante inferior direito do abdômen e eosinofilia periférica. Embora a doença seja de

Infección por agallas y producción de inflorescencias en el arbusto Schinus patagonicus

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Barrancos, María L; Moncaglieri, Romina; Farji-Brener, Alejandro G

2008-01-01

El daño foliar generalmente afecta negativamente la reproducción de las plantas. Sin embargo, se conoce poco sobre cómo la infección por agallas influye en dicho proceso. Analizamos la relación entre la cantidad de agallas foliares causadas por insectos y la producción de inflorescencias en el arbusto Schinus patagonicus. En 63 arbustos localizados en bosques del norte de la Patagonia estimamos el promedio de agallas por hoja y de inflorescencias asociadas por rama. Un incremento en el número...

Incidência de viroses e enfezamentos e estimativa de perdas causadas por molicutes em milho no Paraná Occurrence of viruses and stunting diseases and estimative of yield losses by mollicutes in corn in Paraná State, Brazil

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Elizabeth de Oliveira

2003-01-01

Full Text Available O objetivo deste trabalho foi avaliar a incidência de viroses e enfezamentos e estimar as perdas causadas por enfezamentos na cultura do milho safrinha. Os diagnósticos baseados em sintomas foram confirmados por PCR ou RTPCR. Em todas as lavouras, foram identificadas plantas com sintomas de enfezamentos, em incidência de 6,2% a 49,9% (média de 20,7%. Na identificação de insetos vetores desses patógenos, a cigarrinha Dalbulus maidis foi detectada em 20 lavouras das 24 amostradas, constituindo 66,6% do total de espécimens de cigarrinhas coletadas. A perda potencial causada pelos enfezamentos no período foi estimada em cerca de 16,5 milhões de dólares. A ocorrência de plantas com sintomas de "Maize rayado fino virus" e "Maize dwarf mosaic virus" foi baixa e o diagnóstico confirmado por RTPCR. A análise de 441 amostras suspeitas de infecção por "Mal de Río Cuarto virus", por DASELISA, mostrou ausência desse vírus. Resultados de PCR indicaram a presença de um possível fitoplasma distinto de "Maize bushy stunt phytoplasma" em duas plantas apresentando nanismo acentuado, folhas estreitas, enrijecidas, com deformações, e grãos na inflorescência, havendo necessidade de mais estudos para a confirmação da identidade desse possível novo fitoplasma.The objective of this work was to evaluate the occurence and yield losses by corn stunting diseases and maize viruses in "safrinha" season. Disease diagnostics based on plant symptoms were confirmed by PCR or RTPCR assays. Insect samples were collected in 24 fields for identification of vectors of the pathogens. Corn stunting diseases symptoms were observed in all crops evaluated, with incidence levels ranging from 6.2% to 49.9% (average 20.7% and the presence of the leafhopper Dalbulus maidis, was detected in 20 of the 24 areas evaluated. This insect species was prevalent, representing 66,6% of total leafhoppers specimens collected. The potential yield losses caused by mollicutes was

Restrições causadas pela incontinência urinária à vida da mulher Restricciones ocasionadas por la incontinencia urinaria en la vida de la mujer Urinary incontinence restrictions in women's life

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Maria Helena Baena de Moraes Lopes

2006-03-01

Full Text Available O objetivo deste estudo foi identificar as restrições causadas pela incontinência urinária (IU à vida da mulher, considerando-se o tipo de incontinência, e verificar como manejam este problema. Trata-se de uma análise secundária de dados obtidos em estudo anterior, coletados por meio de entrevista com perguntas abertas e fechadas. A amostra foi composta por 164 mulheres com queixa de IU, internadas em clínicas de ginecologia e urologia de dois hospitais-escola da cidade de Campinas, SP. Apresentavam idade entre 25 e 85 anos e 104 (64% referiram uma ou mais restrições. Citaram alterações nas atividades sexuais (40,9%, sociais (33,5%, domésticas (18,9% e ocupacionais (15,2%. A incontinência urinária mista e a de urgência forem as que mais afetavam a vida das mulheres. A estratégia mais utilizada para minimizar a IU foi o uso de forro e/ou absorvente higiênico. Concluiu-se que a IU tem implicações negativas no cotidiano dessas mulheres.En este estudio se tuvo como objetivo identificar las restricciones causadas por la incontinencia urinaria (IU en la vida de la mujer, considerándose el tipo de incontinencia, y verificar cómo manejan este problema. Se trata de un análisis secundario de datos obtenidos en un estudio anterior, recolectados por medio de entrevista con preguntas abiertas y cerradas. La muestra estuvo compuesta por 164 mujeres con queja de IU, internadas en clínicas de ginecología y urología de dos hospitales docentes de la ciudad de Campinas, SP. Las mujeres tenían entre 25 y 85 años de edad, habiendo relatado 104 (64% de ellas una o más restricciones. Citaron alteraciones en las actividades sexuales (40,9%, sociales (33,5%, domésticas (18,9% y ocupacionales (15,2%. La incontinencia urinaria mixta y la de urgencia fueron las que más afectaban la vida de las mujeres. La estrategia más utilizada para minimizar la IU fue el uso de paños y/o toallas higiénicas. Se concluyó que la IU tiene implicancias

Avaliação lectino-histoquímica de fígado e rim de ovinos com fotossensibilização causada por Brachiaria decumbens

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Samay Z.R. Costa

2013-08-01

Full Text Available Embora sejam as forrageiras mais importantes para a pecuária de corte (bovinocultura de corte no Brasil, em certas épocas ou condições, Brachiaria spp. podem ser tóxicas e causar surtos de fotossensibilização hepatógena que determinam significativas perdas econômicas. Animais que se alimentam em pastos de Brachiaria spp. comumente apresentam macrófagos espumosos isolados ou agrupados, além de cristais no interior de ductos biliares. Saponinas esteroidais têm sido identificadas nestes cristais e são responsabilizadas por lesar o fígado levando ao acúmulo de filoeritrina. Por vezes, imagens negativas desses cristais podem estar presentes no citoplasma de macrófagos espumosos. A patogênese de formação e o tipo de material armazenado nas células espumosas ainda são desconhecidos. A técnica de lectino-histoquímica visa auxiliar na detecção desses macrófagos e, consequentemente, no diagnóstico, além de identificar quais os resíduos de açúcares específicos que estão presentes no citoplasma das células espumosas. Assim, este trabalho teve por objetivo identificar quais lectinas são mais indicadas na detecção de saponinas esteroidais no fígado e rim de ovinos com fotossensibilização causada por Brachiaria decumbens. Fragmentos de fígado e rim de quinze ovinos, de ambos os sexos e idade variável, oriundos de Conceição do Mato Dentro, Minas Gerais, e um ovino mantido em pastagem livre de Brachiaria spp. foram avaliados pela técnica de lectino-histoquímica. Quatorze lectinas foram utilizadas (Con-A, DBA, SBA, PNA, SJA, RCA-I, UEA-I, WGA, SWGA, GSL, PSA, PHA-L, PHA-E e LCA. Verificou-se que, no fígado de ovinos com fotossensibilização provocada pela ingestão de Brachiaria decumbens, a lectina PNA apresentou especificidade e acentuada reatividade aos macrófagos espumosos, bem como especificidade e leve reatividade aos hepatócitos; a lectina WGA teve especificidade e moderada reatividade aos macr

Ações do enfermeiro na identificação precoce de alterações sistêmicas causadas pela sepse grave

Directory of Open Access Journals (Sweden)

Felipe Garrido

2017-04-01

Full Text Available Introdução: As unidades de terapia intensiva (UTIs têm sido organizadas como setores estratégicos para o suporte especializado de assistência ao paciente grave. Objetivo: Verificar as ações do enfermeiro para a identificação precoce das alterações sistêmicas causadas pela sepse grave relacionadas às alterações hemodinâmicas, neurológicas, respiratórias, renais e nutricionais dos pacientes internados em UTIs adulto. Métodos: Estudo descritivo com 24 enfermeiros. Os dados foram coletados por meio de formulário composto de questões estruturadas. Resultados: Apenas 36% dos enfermeiros possuem especialização em UTI adulto; verificou-se que os profissionais identificam parcialmente os sinais e sintomas apresentados pelo paciente séptico. Conclusão: Os enfermeiros encontram dificuldade na identificação precoce das alterações sistêmicas causadas pela sepse grave relacionada às alterações hemodinâmicas, neurológicas, respiratórias, renais e nutricionais dos pacientes internados em UTI adulto, o que pode estar relacionado com a falta de treinamento e de protocolos estabelecidos pelas instituições.

Maize bush stunt and corn stunt: Diseases of corn caused by molicutes/ Enfezamentos vermelho e pálido: Doenças em milho causadas por molicutes

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Nelson Sidnei Massola Júnior

2001-05-01

Full Text Available Maize Bushy Stunt and Corn Stunt are diseases of corn caused respectively by a phytoplasma (maize bushy stunt phytoplasma and by Spiroplasma kunkelii. Both agents are restricted to the floem vessels of diseased plants. The leafhopper Dalbulus maidis is the vector of the diseases. The diseases are very harmfull to corn crops and can cause severe losses. Their importance increased very much in the last years mainly due to the continuous crops, which allow the perpetuation of corn, pathogens and vector over the year. Diseased plants show shortening, redening or yellowing and excessive proliferation of ears, among others symptoms. However, symptoms are variable according to the causal agent, environmental conditions and corn genotype. Correct diagnosis of the diseases has been reached with ELISA (“enzyme-linked immunosorbent assay” and PCR (“polymerase chain reaction”. The use of resistant genotypes of corn is the main strategy of control of the diseases.Os enfezamentos vermelho e pálido são doenças do milho causadas, respectivamente, por um fitoplasma (“maize bushy stunt phytoplasma” e pelo Spiroplasma kunkelii, organismos restritos ao floema das plantas infectadas. Ambas são transmitidas pela cigarrinha Dalbulus maidis. São doenças bastante destrutivas, podendo causar sérios prejuízos aos agricultores. A importância dessas doenças aumentou muito nos últimos anos, devido principalmente aos cultivos “safrinha”, que perpetuam o milho, os patógenos e o vetor no campo durante o ano todo. As plantas doentes exibem redução de crescimento, avermelhamento ou amarelecimento, proliferação excessiva de espigas pequenas e improdutivas, além de outros sintomas. No entanto, esses sintomas dependem do agente causal, condições climáticas e genótipo do milho. A diagnose correta tem sido feita por testes de ELISA (“enzyme-linked immunosorbent assay” e por PCR (“polymerase chain reaction”, devido à complexidade da

Prevalencia y factores de riesgo para infecciones del tracto urinario de inicio en la comunidad causadas por Escherichia coli productor de betalactamasas de espectro extendido en Colombia

Science.gov (United States)

Blanco, Victor M.; Maya, Juan J.; Correa, Adriana; Perenguez, Marcela; Muñoz, Juan S.; Motoa, Gabriel; Pallares, Christian J.; Rosso, Fernando; Matta, Lorena; Celis, Yamile; Garzon, Martha; Villegas, y María V.

2016-01-01

RESUMEN Introducción Las infecciones del tracto urinario (ITU) son frecuentes en la comunidad. Sin embargo, la información de aislamientos resistentes en este contexto es limitada en Latinoamérica. Este estudio tiene como objetivo determinar la prevalencia y los factores de riesgo asociados con ITU de inicio en la comunidad (ITU-IC) causadas por Escherichia coli productor de betalactamasas de espectro extendido (BLEE) en Colombia. Materiales y métodos Entre agosto y diciembre de 2011 se realizó un estudio de casos y controles en 3 instituciones de salud de tercer nivel en Colombia. Se invitó a participar a todos los pacientes admitidos a urgencias con diagnóstico probable de ITU-IC, y se les pidió una muestra de orina. En los aislamien-tos de E. coli se realizaron pruebas confirmatorias para BLEE, susceptibilidad antibiótica, caracterización molecular (PCR en tiempo real para genes bla, repetitive element palindromic PCR [rep-PCR], multilocus sequence typing [MLST] y factores de virulencia por PCR). Se obtuvo información clínica y epidemiológica, y posteriormente se realizó el análisis estadístico. Resultados De los 2.124 pacientes seleccionados, 629 tuvieron un urocultivo positivo, en 431 de estos se aisló E. coli, 54 fueron positivos para BLEE y 29 correspondieron a CTX-M-15. La mayoría de los aislamientos de E. coli productor de BLEE fueron sensibles a ertapenem, fosfomicina y amikacina. La ITU complicada se asoció fuertemente con infecciones por E. coli productor de BLEE (OR = 3,89; IC 95%: 1,10–13,89; p = 0,03). E. coli productor de CTX-M-15 mostró 10 electroferotipos diferentes; de estos, el 65% correspondieron al ST131. La mayoría de estos aislamientos tuvieron 8 de los 9 factores de virulencia analizados. Discusión E. coli portador del gen blaCTX-M-15 asociado al ST131 sigue siendo frecuente en Colombia. La presencia de ITU-IC complicada aumenta el riesgo de tener E. coli productor de BLEE, lo cual debe tenerse en cuenta para ofrecer

“Tipificación molecular y diversidad genética de Mycobacterium spp”

OpenAIRE

Marín Hernández, David

2012-01-01

La Tuberculosis sigue siendo la enfermedad infecciosa con mayor prevalencia en el mundo con un tercio de la población infectada por algún miembro del complejo Mycobacterium tuberculosis. La enfermedad se ha expandido en los países en desarrollo y para el 2005 en México la incidencia fue de 15,249 nuevos casos pulmonares confirmados en el laboratorio. Aunque Mycobacterium tuberculosis es el agente causal principal de la tuberculosis humana, se han reportado continuamente caso...

Utilização da vacina Escherichia coli J5 na imunização de vacas leiteiras contra mastites causadas por E. coli

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Lívio R. Molina

2013-03-01

Full Text Available Avaliou-se a utilização da vacina Escherichia coli J5, na imunização de vacas leiteiras, para prevenção e controle da mastite causada por E. coli através da análise da prevalência das infecções intramamárias (IMM no pós-parto, ocorrência e intensidade dos casos clínicos de mastite nos primeiros 100 dias de lactação, influência na contagem de células somáticas (CCS e produção de leite. O grupo experimental foi composto de 187 animais, divididos em 2 grupos, vacas vacinadas e não vacinadas. As imunizações ocorreram 60 dias antes do parto, 30 dias antes do parto e na primeira semana pós-parto. No dia da secagem e sete dias após o parto foram coletadas amostras para diagnóstico microbiológico dos patógenos causadores de mastite. A ocorrência de casos clínicos foi verificada pelo teste da caneca durante as ordenhas sendo registrados os dados relacionados à intensidade. Amostras foram coletadas mensalmente, a partir do décimo dia de lactação, para avaliação da CCS. A produção de leite foi registrada mensalmente nos primeiros 100 dias de lactação. Verificou-se no grupo vacinado, redução na prevalência de E. coli no pós-parto, na ocorrência de casos clínicos por E.coli nos primeiros 100 dias de lactação bem como na intensidade destes casos clínicos. Não foram observadas diferenças estatísticas significativas na CCS, entretanto vacas vacinadas apresentaram maior produção de leite, comparadas às vacas não vacinadas. A vacinação com E. coli J5 foi eficaz em reduzir a prevalência de infecções intramamárias (IMM ao parto, ocorrência e intensidade dos casos clínicos e aumento na produção de leite nos primeiros 100 dias de lactação.

Fagoterapia frente a infecciones por Staphylococcus aureus meticilino resistente en ratones

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Jesús H Tamariz

Full Text Available Objetivos: Evaluar la actividad de los bacteriófagos frente a infecciones localizadas y sistémicas producidas por Staphylococcus aureus resistente a meticilina (MRSA Materiales y métodos. Se realizó un estudio de tipo experimental en 45 ratones de la cepa Balb/c divididos en nueve grupos de cinco individuos. Se aislaron diez bacteriófagos nativos a partir de muestras clínicas y efluentes hospitalarios, se evaluó su capacidad lítica y su espectro de actividad, en base a lo cual se seleccionaron seis fagos para los ensayos de fagoterapia. Adicionalmente, se empleó un bacteriófago de origen comercial. La fagoterapia fue evaluada mediante profilaxis y terapia de infecciones localizadas y sistémicas causadas por la inoculación de MRSA por vía subcutánea y endovenosa respectivamente. Se probó la efectividad de tres esquemas terapéuticos: monoterapia, cóctel de fagos en múltiples dosis y de cóctel de fagos en una sola dosis. También se comparó la actividad terapéutica de los fagos frente a vancomicina y clindamicina. Resultados. El cóctel de fagos y la terapia a diversas dosis fueron efectivos para prevenir y controlar infecciones localizadas por MRSA, su actividad fue similar a la de vancomicina y clindamicina. La dosis única del cóctel de fagos no logró controlar la infección localizada; asimismo, la fagoterapia no resultó efectiva en infecciones sistémicas. Conclusiones. La fagoterapia se proyecta como una alternativa viable frente a infecciones causadas por MRSA. Se requieren estudios que evalúen aspectos relacionados con la inocuidad de los fagos frente al paciente

Paratuberculosis in ruminants in Brasil: a review

OpenAIRE

Yamasaki, Elise M.; Brito, Marilene F.; Mota, Rinaldo A.; McIntosh, Douglas; Tokarnia, Carlos H.

2013-01-01

A paratuberculose ou doença de Johne é uma enterite granulomatosa causada por Mycobacterium avium subsp. paratuberculosis (Map) e comumente afeta ruminantes domésticos, no entanto, pode infectar várias espécies de mamíferos. Está presente nos cinco continentes e é considerada endêmica em algumas regiões pela Organização Internacional de Epizootias (OIE). Pertence à lista de enfermidades notificáveis, que compreende as doenças transmissíveis de importância sócio-econômica e/ou em saúde-pública...

Paratuberculose em ruminantes no Brasil

OpenAIRE

Yamasaki,Elise M.; Brito,Marilene F.; Mota,Rinaldo A.; McIntosh,Douglas; Tokarnia,Carlos H.

2013-01-01

A paratuberculose ou doença de Johne é uma enterite granulomatosa causada por Mycobacterium avium subsp. paratuberculosis (Map) e comumente afeta ruminantes domésticos, no entanto, pode infectar várias espécies de mamíferos. Está presente nos cinco continentes e é considerada endêmica em algumas regiões pela Organização Internacional de Epizootias (OIE). Pertence à lista de enfermidades notificáveis, que compreende as doenças transmissíveis de importância sócio-econômica e/ou em saúde-pública...

Podridão em cravo causada por Erwinia carotovora subsp. carotovora no Brasil.

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Irene M. G. Almeida

2000-05-01

Full Text Available De fevereiro a abril de 1999, coletaram-se estacas e mudas de cravo (Dianthus caryophyllus em propriedades dos municípios paulistas de Atibaia e Santo Antônio de Posse. Esse material apresentava sintomas caracterizados por não-emissão de raízes ou por podridão de raízes, colo e folhas basais, diferindo daqueles da doença denominada "slow wilt" e dos de escurecimento de vasos e necrose na região do colo, haste e folhas, já relatados em cravo. A partir de material com tais sintomas, isolaram-se bactérias, caracterizadas, mediante testes bioquímicos, culturais, fisiológicos e de patogenicidade, como Erwinia carotovora subsp. carotovora. Trata-se do primeiro relato desse patógeno em cravo no Brasil.

Compactação causada pelo manejo de plantas invasoras em latossolo vermelho-amarelo cultivado com cafeeiros

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Paula Sant'Anna Moreira Pais

2011-12-01

Full Text Available O manejo de plantas invasoras é considerado uma das principais atividades que promovem degradação da estrutura do solo em lavouras cafeeiras, devido à compactação do solo causada pelas operações de controle de plantas invasoras. O objetivo deste estudo foi determinar o modelo de capacidade de suporte de carga para o manejo de plantas invasoras sem capina, bem como, utilizando esse modelo, qual manejo de plantas invasoras causa menor ou maior compactação do solo. Este estudo foi realizado em um Latossolo Vermelho-Amarelo (LVA cultivado com cafeeiros da cutivar Topázio MG 1190 desde 2006, localizado na Fazenda Experimental da EPAMIG, na comunidade Farias, em Lavras-MG. Os manejos de plantas invasoras avaliados foram: na linha de tráfego da entrelinha - grade de discos, herbicida de pós-emergência, herbicida de pré-emergência, roçadora e trincha; e no centro da entrelinha, onde não houve tráfego - amendoim forrageiro (Arachis pintoi, braquiária (Brachiaria decumbens, capina manual, crotalária (Crotalaria juncea e soja (Glicine max L. A amostragem consistiu de duas etapas: uma para determinar o modelo de capacidade de suporte de carga para o manejo de plantas invasoras sem capina; e outra para avaliar a compactação promovida pelos outros manejos de plantas invasoras. A fim de determinar o modelo de capacidade de suporte de carga para o manejo sem capina, foram coletadas no centro da entrelinha 20 amostras com estrutura indeformada nas profundidades de 0-3, 10-13 e 25-28 cm, totalizando 60 amostras. Essas amostras foram submetidas ao ensaio de compressão uniaxial para obter as pressões de pré-consolidação e as umidades volumétricas, que foram usadas para determinar o modelo de capacidade de suporte de carga. Para determinar a compactação causada pelos manejos de plantas invasoras, realizados por meio do controle mecânico, foram coletadas em janeiro de 2010, nas linhas de tráfego das entrelinhas, 180 amostras com

Perfil clínico y parasitológico de la malaria por Plasmodium falciparum y Plasmodium vivax no complicada en Córdoba, Colombia.

OpenAIRE

Angélica Knudson Ospina; Ricardo Sánchez Pedraza; Manuel Alberto Pérez Mazorra; Liliana Jazmín Cortés Cortés; Ángela Patricia Guerra Vega; Rubén Santiago Nicholls Orejuela

2015-01-01

Antecedentes. En Colombia existen pocos estudios que buscan encontrar diferencias clínicas y parasitológicas en la malaria causada por Plasmodium falciparum y Plasmodium vivax.  Objetivo. Describir el perfil clínico y parasitológico de las malarias por Plasmodium falciparum y Plasmodium vivax no complicadas en Tierralta, Córdoba, Colombia. Materiales y métodos. Se evaluaron pacientes con paludismo no complicado por Plasmodium falciparum y Plasmodium vivax según los protocolos estandariz...

Comparison of the pain severity, drug leakage and ecchymosis rates caused by the application on tramadol intramuscular injection in Z-track and Air-lock techniques Comparación de la severidad del dolor y las tasas de escape de líquido y equimosis causadas por la aplicación intramuscular de tramadol usando las técnicas de cámara de aire y Z-track Comparação da severidade da dor e as taxas de fuga de líquidas equimoses causadas pela aplicação intramuscular de tramadol usando as técnicas de câmaras de ar e Z-track

Directory of Open Access Journals (Sweden)

Shahla Najafidolatabad

2010-07-01

Full Text Available Objective. To compare the pain severity caused by the application of the Z-track and Air-lock intramuscular injections. Methodology. Unblinded clinical trial, where 90 female subjects aged between 18 and 60 years old were randomly assigned to two groups; the first group received the tramadol intramuscular injection using the Z-track technique (ZT and the second group received it through the air- lock method (AL. A 10 centimeter linear visual scale was used to evaluate the injection pain. The scale length was considered as the pain severity. Data were analyzed using the SPSS version 13. Groups' age, Body Mass index (BMI, and pain intensity were compared using unpaired student's t test. Results. The study showed that the pain severity of patients in the AL method group was lower than in the ZT technique group (pObjetivo. Comparar la severidad del dolor y las tasas de escape de líquido y equimosis causadas por la aplicación de las inyecciones intramusculares usando la técnica Z-track (ZT y la técnica de la cámara de aire (CA. Metodología. Este es un ensayo clínico no ciego donde un grupo de 90 mujeres entre los 18 y 60 años fueron aleatoriamente asignadas a dos grupos; El primer grupo recibió la inyección por medio de la técnica ZT y el segundo por medio de la técnica de CA. Se uso una escala visual lineal de 10 centímetros para medir la intensidad del dolor causado por la inyección. La longitud de la escala fue considerada como la severidad del dolor. Los datos fueron analizados utilizando el programa SPSS versión 13. La edad, el índice de Masa Corporal (IMC, y la intensidad del dolor de los grupos fueron comparados usando el test de la t para muestras independientes. Resultados. El estudio mostró que la severidad del dolor y las tasas de escape de líquido y equimosis en las pacientes evaluadas usando el método CA era menor que el dolor causado por la técnica ZT (p Objetivo. Comparar a severidade da dor e as taxas de escape de

Polymorphisms of twenty regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis

Science.gov (United States)

Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans or animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and the other members o...

The Mycobacterium tuberculosis homologue of the Mycobacterium ...

African Journals Online (AJOL)

With the completion of genome sequencing of Mycobacterium tuberculosis and upsurge in the incidence of M. tuberculosis infection worldwide partly as a result of HIV pandemic, there is need for rationale approach to vaccine and chemotherapy discoveries for M. tuberculosis. The homologue of mig gene of. Mycobacterium ...

Efectos ambientales por el cambio de uso de suelo en la etapa la arboleda, en la urbanizaciòn ciudad celeste, canton samborondon

OpenAIRE

Espol; Jordan Rubio, Joann Eduardo; Piedra Soto, Dimitri

2017-01-01

Este trabajo se enfocó en las consecuencias causadas por el cambio de uso de suelo sobre el ambiente, por la actividad del movimiento de tierra que se realiza para el proceso de construcción de viviendas en la etapa “la arboleda” de la urbanización “ciudad celeste” en el cantón samborondón, provincia del guayas. Guayaquil MAGISTER EN CAMBIO CLIMATICO

Evasión molecular de la activación del macrófago bovino por Mycobacterium avium subespecie paratuberculosis

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René Ramírez G.

2013-11-01

Full Text Available El Mycobacterium avium subespecie paratuberculosis (MAP es el agente causal de una enfermedad granulomatosica crónica, que afecta el tracto gastrointestinal de rumiantes domesticos y salvajes, conocida como la enfermedad de Johne o paratuberculosis. MAP es un microorganismo de crecimiento lento en cultivo, no obstante sobrevive in vivo en células fagocíticas mononucleares de los rumiantes, bajo condiciones de susceptibilidad individual, virulencia de la cepa infectante y estado inmune del individuo afectado. Una vez MAP es fagocitado por el macrófago bovino, tanto el macrófago como MAP activan: el uno para tratar de destruir a MAP y luego sufrir apoptosis y el otro para evadir su destrucción dentro del fagolisosoma del macrófago. El balance de dicha confrontación molecular determina el curso inicial de la infección hacia la eliminación eficiente del microorganismo o hacia el establecimiento de la infección, que culminará en los estadios III (clínico intermitente y IV (clínica terminal de la enfermedad de Johne. En la presente revisión se discuten los diferentes mecanismos moleculares por los cuales MAP evade la respuesta inmune, con énfasis en su comportamiento dentro de la vacuola fagocítica y como el agente establece mecanismos de sobrevivencia intracelular y altera la activación de los macrófagos del hospedero y de la respuesta inmune específica.

Endoftalmitis poscirugía de catarata por Sphingomonas paucimobilis

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Omar Mauri Garrido

Full Text Available Se presenta la caracterización y manejo terapéutico de un caso de endoftalmitis bacteriana posoperatoria causada por el germen Sphingomonas paucimobilis. La endoftalmitis es la inflamación de los tejidos intraoculares, considerada como la más devastadora de las complicaciones posoperatorias; posee pronóstico visual muy reservado y un elevado riesgo de secuela. Las Sphingomonas paucimobilis son bacterias gramnegativas con forma de bacilo, quimioheterótrofa y estrictamente aerobias que causan enfermedades en los seres humanos, principalmente infecciones hospitalarias que típicamente son tratadas fácilmente con antibióticos. Por sus capacidades biodegradantes y biosintéticas, son pocos los reportes hallados de infección intraocular por este germen. El pronóstico visual es favorable con un diagnóstico precoz y la aplicación del tratamiento adecuado. En este artículo se presentan un caso de endoftalmitis poscirugía de catarata por Sphingomonas paucimobilis reportado en Cuba en el mes de septiembre de 2009.

Cambiando los paradigmas de la infección por M. pneumoniae en pediatría

OpenAIRE

Jocelin Merida-Vieyra; Alejandra Aquino-Andrade; Rosa María Ribas-Aparicio; Agustín de Colsa-Ranero

2017-01-01

La neumonía atípica es un término que originalmente se utilizó en pacientes adultos que presentaban neumonías de curso atípico, generalmente leves y de evolución benigna, que eran causadas por agentes como: Mycoplasma pneumoniae, Chlamydophila pneumoniae y Legionella pneumophila. En población pediátrica la presentación, tanto clínica como epidemiológica, de las neumonías por M. pneumoniae merece una especial atención ya que no siempre son cursos benignos y pueden existir numerosos cuadros ext...

Mycobacterium saopaulense sp. nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae--Mycobacterium abscessus group.

Science.gov (United States)

Nogueira, Christiane Lourenço; Whipps, Christopher M; Matsumoto, Cristianne Kayoko; Chimara, Erica; Droz, Sara; Tortoli, Enrico; de Freitas, Denise; Cnockaert, Margo; Palomino, Juan Carlos; Martin, Anandi; Vandamme, Peter; Leão, Sylvia Cardoso

2015-12-01

Five isolates of non-pigmented, rapidly growing mycobacteria were isolated from three patients and,in an earlier study, from zebrafish. Phenotypic and molecular tests confirmed that these isolates belong to the Mycobacterium chelonae-Mycobacterium abscessus group, but they could not be confidently assigned to any known species of this group. Phenotypic analysis and biochemical tests were not helpful for distinguishing these isolates from other members of the M. chelonae–M.abscessus group. The isolates presented higher drug resistance in comparison with other members of the group, showing susceptibility only to clarithromycin. The five isolates showed a unique PCR restriction analysis pattern of the hsp65 gene, 100 % similarity in 16S rRNA gene and hsp65 sequences and 1-2 nt differences in rpoB and internal transcribed spacer (ITS) sequences.Phylogenetic analysis of a concatenated dataset including 16S rRNA gene, hsp65, and rpoB sequences from type strains of more closely related species placed the five isolates together, as a distinct lineage from previously described species, suggesting a sister relationship to a group consisting of M. chelonae, Mycobacterium salmoniphilum, Mycobacterium franklinii and Mycobacterium immunogenum. DNA–DNA hybridization values .70 % confirmed that the five isolates belong to the same species, while values ,70 % between one of the isolates and the type strains of M. chelonae and M. abscessus confirmed that the isolates belong to a distinct species. The polyphasic characterization of these isolates, supported by DNA–DNA hybridization results,demonstrated that they share characteristics with M. chelonae–M. abscessus members, butconstitute a different species, for which the name Mycobacterium saopaulense sp. nov. is proposed. The type strain is EPM10906T (5CCUG 66554T5LMG 28586T5INCQS 0733T).

Evitar o efeito da vacinação BCG na detecção de infecção por Mycobacterium tuberculosis com um teste sanguíneo Avoiding the effect of BCG vaccination in detecting Mycobacterium tuberculosis infection with a blood test

OpenAIRE

R. Diel; M Ernst; G Doscher; L Visuri-Karbe; U Greinert; S Niemann; A Nienhaus; C Lange

2007-01-01

O risco de progressão da infecção por Mycobacterium tuberculosis para tuberculose-doença é sobretudo elevado nos primeiros anos após a infecção, estimando-se que 50% dos casos de tuberculose-infecção desenvolvem doença nos dois anos seguintes. A investigação precoce dos contactos baseia-se neste facto, e o teste cutâneo de tuberculina tem sido largamente utilizado como a prova de rastreio, apesar da sua limitação na população vacinada com BCG, nas reacções cruzadas com micobactérias não tuber...

Pansinusitis y afectación intracraneal por implante dental Pansinusitis and intracranial impact of a dental implant

Directory of Open Access Journals (Sweden)

Josep Rubio-Palau

2012-03-01

Full Text Available Las sinusitis odontógenas son una patología relativamente frecuente causada por infecciones dentales, quistes periapicales así como tras procedimientos bucodentales como una endodoncia, una elevación sinusal o la colocación de un implante. A continuación se presenta un caso extremo de una pansinusitis derecha con fistulización a espacio epidural causada por un implante osteointegrado. Ante la sospecha de una sinusitis maxilar de origen odontogénico se debe iniciar rápidamente un tratamiento antibiótico correcto y un seguimiento estrecho ya que pueden tener consecuencias fatales como la pérdida de un ojo, abscesos cerebrales o incluso la muerte.Odontogenic sinusitis is a relatively common disease caused by dental infections, periapical cysts and oral procedures such as root canal, sinus lift or implant placement. We report an extreme case of a right pansinusitis with an epidural space fistula caused by osseointegrated implants. When maxillary sinusitis of odontogenic origin is suspected, we should quickly start effective antibiotic treatment and monitor the patient closely because odontogenic sinusitis can have serious consequences, such as the loss of an eye, brain abscess or death.

Intoxicações por plantas em ruminantes no Brasil e no Uruguai: importância econômica, controle e riscos para a saúde pública

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Riet-Correa Franklin

2001-01-01

Full Text Available As intoxicações por plantas em animais de produção, no Brasil e no Uruguai são conhecidas desde que os pioneiros Espanhóis e Portugueses introduziram as primeiras cabeças de gado em pastagens naturais da região. As perdas econômicas ocasionadas pelas intoxicações por plantas podem ser definidas como diretas ou indiretas. As perdas diretas são causadas pelas mortes de animais, diminuição dos índices reprodutivos (abortos, infertilidade, malformações, redução da produtividade nos animais sobreviventes e outras alterações devidas a doenças transitórias, enfermidades subclínicas com diminuição da produção de leite, carne ou lã, e aumento à susceptibilidade a outras doenças devido a depressão imunológica. As perdas indiretas incluem os custos de controlar as plantas tóxicas nas pastagens, as medidas de manejo para evitar as intoxicações como a utilização de cercas e o pastoreio alternativo, a redução do valor da forragem devido ao atraso na sua utilização, a redução do valor da terra, a compra de gado para substituir os animais mortos, e os gastos associados ao diagnóstico das intoxicações e ao tratamento dos animais afetados (Riet-Correa et al. 1993, James 1994. As perdas econômicas causadas pelas intoxicações por plantas são difíceis de se estimar por que não existem dados confiáveis sobre todos esses componentes, no entanto, as perdas causadas por mortes são fáceis de determinar quando dispomos de dados elaborados por laboratórios de diagnóstico, sobre a freqüência das causas de mortes dos animais numa determinada região.

"O corpo que eu fui e o corpo que eu sou": concepções de mulheres com alterações causadas pela hanseníase

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Iací Proença Palmeira

2012-01-01

Full Text Available Se objetivó comprender las concepciones de las mujeres sobre el cuerpo femenino con alteraciones provocadas por la lepra. Estudio de naturaleza cualitativa y descriptiva, que aplicó los conceptos de la teoría de las representaciones. Participaron cuarenta y tres mujeres con alteraciones corporales causadas por la lepra, frecuentadoras de una unidad de referencia especializada en dermatología sanitaria. Se realizó entrevista semi-estructurada, individual, y análisis temático de contenido. Los resultados muestran concepciones del cuerpo relacionadas a la salud (pasado y a la enfermedad (presente, mostrando la fuerza de la estética y la funcionalidad del cuerpo, influenciando en la participación e inserción social de estas mujeres. Se concluye que las concepciones de las mujeres sobre su cuerpo explican sus prácticas de cuidado de sí, y conocerlas contribuye para que enfermería mejore la atención de las necesidades de estas mujeres.

Desestructuración familiar ocasionada por emigración y su impacto en el rendimiento escolar

OpenAIRE

Pasato Quito, Celia Cristina

2010-01-01

La emigración como proceso de movilización afecta notablemente a nuestra sociedad, la misma que ha dejado huella en todos los miembros de la familia, por esta razón el presente trabajo centra su objetivo en verificar si la desestructuración familiar (ausencia de la presencia física y carencia afectiva) causada por emigración repercute en el rendimiento escolar, en niños de 6 a 12 años del Centro Educativo San Martín , a la vez aborda la situación, la dinámica familiar y las consecuencias ...

Clasificación del reflejo óculo-cardiaco por la desviación centesimal de viola

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Luis Rubén Paz B.

1947-09-01

Full Text Available Desde mucho tiempo antes, habían notado los observadores, las modificaciones reflejas de las funciones vegetativas causadas por la excitación de algún nervio sensitivo y aun habían deducido de esta observación, aplicaciones prácticas, como la cura operatoria de las lesiones nasales en el tratamiento de algunos casas de asma (W. Hack a explicaciones patogénicas, como la atribución del sincope del principio de la anestesia por inhalación a la brusca irritación de las terminaciones sensitivas de la pituitaria (Knoll y Kratschmer.

Photodynamic inactivation of the models Mycobacterium phlei and Mycobacterium smegmatis in vitro

Science.gov (United States)

Bruce-Micah, R.; Gamm, U.; Hüttenberger, D.; Cullum, J.; Foth, H.-J.

2009-07-01

Photodynamic inactivation (PDI) of bacterial strains presents an attractive potential alternative to antibiotic therapies. Success is dependent on the effective accumulation in bacterial cells of photochemical substances called photosensitizers, which are usually porphyrins or their derivatives. The kinetics of porphyrin synthesis after treatment with the precursor ALA and the accumulation of the Chlorin e6 and the following illumination were studied. The goal was to estimate effectivity of the destructive power of these PS in vitro in respect of the physiological states of Mycobacteria. So the present results examine the cell destruction by PDI using ALA-induced Porphyrins and Chlorin e6 accumulated in Mycobacterium phlei and Mycobacterium smegmatis, which serve as models for the important pathogens Mycobacterium tuberculosis, Mycobacterium leprae and Mycobacterium bovis. We could show that both Mycobacterium after ALA and Chlorin e6 application were killed by illumination with light of about 662 nm. A reduction of about 97% could be reached by using a lightdose of 70 mW/cm2.

In vitro Inhibition of Mycobacterium smegmatis and Mycobacterium ...

African Journals Online (AJOL)

Some Nigerian plants used in traditional medicine to treat tuberculosis and/or some of its symptoms were screened for in vitro activity against Mycobacterium smegmatis and a clinical isolate of Mycobacterium tuberculosis. Only 3 of the 6 crude methanolic extracts of the 6 plant species exhibited inhibitory activities against ...

Whole-genome sequence analysis of the Mycobacterium avium complex and proposal of the transfer of Mycobacterium yongonense to Mycobacterium intracellulare subsp. yongonense subsp. nov.

Science.gov (United States)

Castejon, Maria; Menéndez, Maria Carmen; Comas, Iñaki; Vicente, Ana; Garcia, Maria J

2018-06-01

Bacterial whole-genome sequences contain informative features of their evolutionary pathways. Comparison of whole-genome sequences have become the method of choice for classification of prokaryotes, thus allowing the identification of bacteria from an evolutionary perspective, and providing data to resolve some current controversies. Currently, controversy exists about the assignment of members of the Mycobacterium avium complex, as is for the cases of Mycobacterium yongonense and 'Mycobacterium indicus pranii'. These two mycobacteria, closely related to Mycobacterium intracellulare on the basis of standard phenotypic and single gene-sequences comparisons, were not considered a member of such species on the basis on some particular differences displayed by a single strain. Whole-genome sequence comparison procedures, namely the average nucleotide identity and the genome distance, showed that those two mycobacteria should be considered members of the species M. intracellulare. The results were confirmed with other whole-genome comparison supplementary methods. According to the data provided, Mycobacterium yongonense and 'Mycobacterium indicus pranii' should be considered and renamed and included as members of M. intracellulare. This study highlights the problems caused when a novel species is accepted on the basis of a single strain, as was the case for M. yongonense. Based mainly on whole-genome sequence analysis, we conclude that M. yongonense should be reclassified as a subspecies of Mycobacterium intracellulareas Mycobacterium intracellularesubsp. yongonense and 'Mycobacterium indicus pranii' classified in the same subspecies as the type strain of Mycobacterium intracellulare and classified as Mycobacterium intracellularesubsp. intracellulare.

Algunos aspectos relacionados con las enfermedades tropicales transmitidas por moluscos

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A. Mijail Pérez

1999-12-01

Full Text Available Muchas especies de moluscos son hospedantes de diversos parásitos que provocan infecciones muy serias e incluso mortales en seres humanos y ganado. La esquistosomiasis extendida por 74 países, infecta a unos 200 millones de personas y está en segundo lugar, después de la malaria, como causa de la mortalidad humana originada por parásitos. La infección es causada por un platelminto que se desarrolla en caracoles de agua dulce y que desde el agua puede penetrar en la piel de un ser humano y desarrollarse dentro de sus órganos, produciendo distintos grados de infección, hasta provocar la muerte. Distintas especies de esos caracoles están extendidas por Centro América. El cólera, otra enfermedad que también es mortal, se ha encontrado en moluscos bivalvos comestibles colectados en el Golfo de Nicoya, que bordea Nicaragua y Costa Rica Estas epidemias se pueden combatir con diversos métodos de control de los caracoles hospedantes de parásitos.

Imunidade conferida por vacinas anti-meningocócicas

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Milagres Lucimar Gonçalves

1993-01-01

Full Text Available Em razão da recente epidemia de doença meningocócica causada por N. meningitidis B na Grande São Paulo, Brasil, foi feita revisão das epidemias dessa doença ocorridas no Brasil desde o início do século e uma análise das vacinas atuais contra N. meningitidis A, C, Y e W135. Também são discutidos os mais recentes avanços no desenvolvimento e aplicação de vacina contra M meningitidis B, um desafio constante para os maiores centros de pesquisa de todo o mundo.

Mycobacterium persicum sp. nov., a novel species closely related to Mycobacterium kansasii and Mycobacterium gastri.

Science.gov (United States)

Shahraki, Abdolrazagh Hashemi; Trovato, Alberto; Mirsaeidi, Mehdi; Borroni, Emanuele; Heidarieh, Parvin; Hashemzadeh, Mohamad; Shahbazi, Narges; Cirillo, Daniela M; Tortoli, Enrico

2017-06-01

Four strains isolated in Iran from pulmonary specimens of unrelated patients are proposed as representative of a novel Mycobacterium species. Similarity, at the phenotypic level, with Mycobacterium kansasii is remarkable with the photochromogenic yellow pigmentation of the colonies being the salient feature. They differ, however, genotypically from this species and present unique sequences in 16S rRNA, hsp65 and rpoB genes. The average nucleotide identity and the genome-to-genome distance fully support the status of an independent species. The name proposed for this species is Mycobacterium persicum sp. nov. with AFPC-000227T (=DSM 104278T=CIP 111197T) as the type strain.

Agnosia integrativa causada por una epilepsia focal occipital izquierda: estudio de caso

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Óscar Mauricio Aguilar Mejía

2011-01-01

Full Text Available La agnosia integrativa es un tipo de agnosia visual asociada a lesiones occipitales bilaterales que se caracteriza por la incapacidad para identificar estímulos en fondos complejos, especialmente, cuando se trata de formas superpuestas o entrelazadas. Existen dificultades para codificar y agrupar el todo a partir de las partes del estímulo. Se presenta un caso de una paciente de 14 años, con una agnosia integrativa como consecuencia de una epilepsia focal sintomática refractaria, con un foco epileptogénico occipital izquierdo. Se plantean las características clínicas de la paciente y se hace un análisis de sus procesos de reconocimiento visual discutidos a partir de los diferentes modelos teóricos que tratan de explicar la percepción visual.

Terapêutica interdisciplinar para fratura cominutiva de côndilo por projétil de arma de fogo: enfoque miofuncional

OpenAIRE

Bianchini,Esther Mandelbaum Gonçalves; Moraes,Rogério Bonfante; Nazario,Daniella; Luz,João Gualberto de Cerqueira

2010-01-01

TEMA: ferimentos causados por projéteis de arma de fogo apresentam alta incidência na região da cabeça e face. A articulação temporomandibular pode estar envolvida, além de estruturas anatômicas importantes como o nervo facial, necessitando de equipe multidisciplinar para efetuar tratamento adequado. PROCEDIMENTOS: apresentação de caso clínico de fratura condilar cominutiva causada por projétil de arma de fogo tratado de forma não-cirúrgica associado à terapia miofuncional orofacial. Paciente...

Miasis orbital severa causada por Cochliomyia hominivorax en la región andina de Ecuador

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Juan Dominguez Enríquez

2016-01-01

Full Text Available Presentamos el caso de una indígena kichwa de 91 años con miasis orbital destructiva que compromete globo ocular derecho con destrucción de tejido óseo orbital, nasal, etmoidal y del seno frontal, confirmado por tomografía axial computarizada y con reconstrucción ósea tridimensional. Infectada en una zona templada a 2,418 m de altitud en la provincia andina de Imbabura, Ecuador. La paciente se presentó en el Hospital de Cotacachi con una tumoración cavitaria en el globo ocular derecho acompañada de secreción maloliente, supurativa, observando y extrayéndose más de 100 larvas de moscas desde su interior. Estas fueron identificadas como Cochliomyia hominivorax, por la disposición de sus espiráculos respiratorios.

Mycobacterium franklinii sp. nov., a species closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group.

Science.gov (United States)

Lourenço Nogueira, Christiane; Simmon, Keith E; Chimara, Erica; Cnockaert, Margo; Carlos Palomino, Juan; Martin, Anandi; Vandamme, Peter; Brown-Elliott, Barbara A; Wallace, Richard; Cardoso Leão, Sylvia

2015-07-01

Two isolates from water, D16Q19 and D16R27, were shown to be highly similar in their 16S rRNA, 16S-23S internal transcribed spacer (ITS), hsp65 and rpoB gene sequences to 'Mycobacterium franklinii' DSM 45524, described in 2011 but with the name not validly published. They are all nonpigmented rapid growers and are related phenotypically and genetically to the Mycobacterium chelonae-Mycobacterium abscessus group. Extensive characterization by phenotypic analysis, biochemical tests, drug susceptibility testing, PCR restriction enzyme analysis of the hsp65 gene and ITS, DNA sequencing of housekeeping genes and DNA-DNA hybridization demonstrated that 'M. franklinii' DSM 45524, D16Q19 and D16R27 belong to a single species that is separated from other members of the M. chelonae-M. abscessus group. On the basis of these results we propose the formal recognition of Mycobacterium franklinii sp. nov. Strain DSM 45524(T) ( = ATCC BAA-2149(T)) is the type strain.

Queratopatía cristalina: diagnóstico clínico y microbiológico de una infección corneal infrecuente causada por el grupo Streptococcus mitis

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Gustavo J Galperín

2011-09-01

Full Text Available El objetivo del presente informe es describir un caso de queratopatía cristalina causada por microorganismos pertenecientes al grupo Streptococcus mitis en una paciente que concurrió a la consulta oftalmológica por molestias en su ojo derecho. Al examen oftalmológico presentó un punto de sutura interrumpida de nylon 10-0 sin tensión y con secreciones mucosas adheridas. El punto flojo fue retirado bajo normas de asepsia. Se indicó colirio de moxifloxacina al 0,5 %; el ojo tuvo una evolución adecuada, con una correcta epitelización. Sin embargo, luego de 15 días desarrolló un infiltrado blanquecino arboriforme. Se tomó una muestra en el quirófano, enhebrando el trayecto intraestromal de la sutura retirada con sutura de vicryl 7-0. Se indicaron colirios de vancomicina con 50 mg/ml. El infiltrado se mantuvo estable durante 45 días, luego se incrementó el tamaño y se produjo necrosis tisular con peligro de perforación corneal. Se realizó un recubrimiento conjuntival bipediculado. La paciente evolucionó favorablemente y luego de la retracción espontánea del recubrimiento, se observó leucoma cicatrizal y neovasos corneales.Crystalline keratopathy: an infrequent corneal infection produced by the Streptococcus mitis group. The objective of this report is to describe a case of crystalline keratopathy caused by the Streptococcus mitis group corresponding to a patient who attended hospital for discomfort in his right eye. The ophthalmological examination showed an interrupted stitch of 10-0 nylon suture without tension and with attached mucus secretions. The loose suture was removed under aseptic conditions. Moxifloxacin 0.5 % eye drops were topically indicated. The treated eye successfully epithelialized and evolved favorably. However, after 15 days, a white tree-shaped infiltrate developed. A corneal sample was taken in the operating room, threading the intrastromal path of the removed stitch with a 7-0 vicryl suture. Vancomycin 50

Facoemulsificación e implante de lente intraocular en cataratas causadas por uveitis Phacoemulsification and intraocular lens implantation in patients with cataract caused by uveitis

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Dayamí Pérez Gómez

2008-06-01

Full Text Available OBJETIVO: Evaluar los resultados de la facoemulsificación y el implante de lente intraocular en cataratas causadas por uveítis. MÉTODOS: Se estudiaron 37 ojos con 6 meses de inactividad inflamatoria, agudeza visual menor de 0,6 y edad media de 47 años. Se excluyeron otras enfermedades que disminuyeran la visión. En el preoperatorio se prescribió antiinflamatorios. Se realizó la facoemulsificación con implante de lente intarocular (PMMA, óptica 6 mm; durante el seguimiento desde 24 h/6 meses se indicó antiinflamatorios esteroideos, no esteroideos e inmunosupresores hasta 6 semanas. RESULTADOS: Se observaron numerosas alteraciones del segmento anterior secundarias a las uveítis padecidas (posquirúrgicas, inespecíficas, ciclítis heterocrómica de Fuchs, postraumáticas, síndrome de Reiter, por toxoplasmosis y otras coroiditis infecciosas y por artritis reumatoide juvenil. No hubo recurrencia posquirúrgica de la uveítis en 9 ojos (24,3 %. Las complicaciones estuvieron relacionadas con la uveítis, la cirugía, la actividad inflamatoria, y la reacción del epitelio capsular. La opacidad capsular posterior apareció en 11 pacientes (29,7 % asociada a edad joven. La evolución individual se consideró satisfactoria en 30 (81,1 % pacientes con mejoría de la visión hasta 0,8-1,0 en 28 (75,6 % y 0,5-0,6 en 2 (5,4 %. CONCLUSIONES: La facoemulsificación con implante de lente intarocular es de gran utilidad en las cataratas posuveítis al condicionar poca agresión quirúrgica, ser controlable la inflamación y proporcionar buenos resultados visuales además del reordenamiento anatomofisiológico del segmento anterior.OBJECTIVE: To assess the results of phacoemulsification and intraocular lens implantation in cataracts caused by uveitis. METHODS: Thirty seven eyes with no inflammation for 6 months, visual acuity below 0.6 and average age of 47 years were studied. Other diseases affecting the vision were excluded. The preoperative

Mycobacterium bovis and Other Uncommon Members of the Mycobacterium tuberculosis Complex.

Science.gov (United States)

Esteban, Jaime; Muñoz-Egea, Maria-Carmen

2016-12-01

Since its discovery by Theobald Smith, Mycobacterium bovis has been a human pathogen closely related to animal disease. At present, M. bovis tuberculosis is still a problem of importance in many countries and is considered the main cause of zoonotic tuberculosis throughout the world. Recent development of molecular epidemiological tools has helped us to improve our knowledge about transmission patterns of this organism, which causes a disease indistinguishable from that caused by Mycobacterium tuberculosis. Diagnosis and treatment of this mycobacterium are similar to those for conventional tuberculosis, with the important exceptions of constitutive resistance to pyrazinamide and the fact that multidrug-resistant and extremely drug-resistant M. bovis strains have been described. Among other members of this complex, Mycobacterium africanum is the cause of many cases of tuberculosis in West Africa and can be found in other areas mainly in association with immigration. M. bovis BCG is the currently available vaccine for tuberculosis, but it can cause disease in some patients. Other members of the M. tuberculosis complex are mainly animal pathogens with only exceptional cases of human disease, and there are even some strains, like "Mycobacterium canettii," which is a rare human pathogen that could have an important role in the knowledge of the evolution of tuberculosis in the history.

The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

KAUST Repository

Phelan, Jody; Maitra, Arundhati; McNerney, Ruth; Nair, Mridul; Gupta, Antima; Coll, Francesc; Pain, Arnab; Bhakta, Sanjib; Clark, Taane G.

2015-01-01

Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.

The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

KAUST Repository

Phelan, Jody

2015-06-04

Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.

The draft genome of Mycobacterium aurum , a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

Directory of Open Access Journals (Sweden)

Jody Phelan

2015-01-01

Full Text Available Mycobacterium aurum (M. aurum is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related to 96.2% for rrs (streptomycin, capreomycin. We observed two homologous genes encoding the catalase-peroxidase enzyme (katG that is associated with resistance to isoniazid. Similarly, two emb B homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum , this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.

Fratura de órbita por queda de cavalo e correção de estrabismo Orbit fracture by horse fall and strabismus correction

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Abelardo de Souza Couto Junior

2010-06-01

Full Text Available Descreve-se uma paciente com fratura orbitária grave causada por queda de cavalo. Relato do seu tratamento cirúrgico com correção do estrabismo e tentativa de correção da enoftamia.Description of a patient with orbital fracture cause by a horse fall. Follow-up the surgical treatment of the strabismus and the enophtlamos.

MycoCAP - Mycobacterium Comparative Analysis Platform.

Science.gov (United States)

Choo, Siew Woh; Ang, Mia Yang; Dutta, Avirup; Tan, Shi Yang; Siow, Cheuk Chuen; Heydari, Hamed; Mutha, Naresh V R; Wee, Wei Yee; Wong, Guat Jah

2015-12-15

Mycobacterium spp. are renowned for being the causative agent of diseases like leprosy, Buruli ulcer and tuberculosis in human beings. With more and more mycobacterial genomes being sequenced, any knowledge generated from comparative genomic analysis would provide better insights into the biology, evolution, phylogeny and pathogenicity of this genus, thus helping in better management of diseases caused by Mycobacterium spp.With this motivation, we constructed MycoCAP, a new comparative analysis platform dedicated to the important genus Mycobacterium. This platform currently provides information of 2108 genome sequences of at least 55 Mycobacterium spp. A number of intuitive web-based tools have been integrated in MycoCAP particularly for comparative analysis including the PGC tool for comparison between two genomes, PathoProT for comparing the virulence genes among the Mycobacterium strains and the SuperClassification tool for the phylogenic classification of the Mycobacterium strains and a specialized classification system for strains of Mycobacterium abscessus. We hope the broad range of functions and easy-to-use tools provided in MycoCAP makes it an invaluable analysis platform to speed up the research discovery on mycobacteria for researchers. Database URL: http://mycobacterium.um.edu.my.

Rickettsiosis transmitidas por garrapatas en las Américas: avances clínicos y epidemiológicos, y retos en el diagnóstico

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Marylin Hidalgo

2013-08-01

Full Text Available Las rickettsiosis son entidades clínicas de tipo zoonótico, causadas por bacterias intracelulares estrictas de los géneros Rickettsia y Orientia, pertenecientes a la familia Rickettsiaceae. Su ecología está determinada por factores ambientales y la presencia de vectores específicos que condicionan el establecimiento y la epidemiología en diferentes regiones del mundo. En las Américas, durante el siglo XX, únicamente eran reconocidas tres de estas enfermedades: la fiebre manchada de las Montañas Rocosas, el tifus epidémico y el tifus endémico. Sin embargo, apartir del año 2000 se han descrito mas de 10 especies diferentes previamente desconocidas en este continente, tanto en artrópodos como en casos clínicos, hecho que permite clasificarlas como entidades clínicas emergentes y reemergentes. Dadas las manifestaciones clínicas de las enfermedades causadas por rickettsias, siendo la gran mayoría inespecíficas y, por lo mismo, compartidas con otras enfermedades infecciosas, especialmente virales y bacterianas, han sido enmarcadas entre los diagnósticos diferenciales del síndrome febril agudo, tanto en áreas urbanas como tropicales. En la actualidad, se cuenta con métodos diagnóstico sdirectos e indirectos, que son útiles en la identificación del agente infeccioso, en este caso, causante de rickettsiosis.   doi: http://dx.doi.org/10.7705/biomedica.v33i0.1466

Mastite bovina causada por Prototheca zopfii: relato de um caso Clinical bovine mastitis caused by Prototheca zopfii: a case study

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Maria Aparecida Vasconcelos Paiva Brito

1997-12-01

Full Text Available Um caso de mastite clínica causada por Prototheca zopfii em uma vaca de um rebanho leiteiro localizado na Zona da Mata do Estado de Minas Gerais foi estudado. O animal apresentou sinais clínicos nos quartos mamárias anteriores e queda acentuada na produção de leite. Após o diagnóstico inicial, o animal foi observado durante onze meses, do início da manifestação da doença até 12 dias após o parto. Exames microbiológicos do leite foram realizadas aos 7, 30, 39, 49, 65, 326 e 331 dias após o isolamento inicial, sendo os dois últimos exames realizados 7 e 12 dias após o parto. As amostras de P. zopfii isoladas apresentaram resistência in vitro a: ampicilina, canamicina, cefatoridina, enrofloxacina, estreptomicina, gentamicina, neomicina, oxacilina, penicilina, sulfonamidas e trimetoprim x sulfametoxazol. Foi avaliada a susceptibilidade in vitro das amostras de P. zopfii a um produto natural constituído de extrato de sementes de frutas cítricas, obtido comercialmente. A menor concentração que inibiu totalmente a alga foi 1:500. Recomendou-se um tratamento com a diluição a 1:200 do extrato em solução fisiológica estéril contendo timerosal a 1:30.000 como conservante, em doses diárias de 20ml, por via intramamária. O tratamento foi realizado inicialmente durante sete dias. Após este período houve redução, mas não a completa eliminação dos organismos do leite. Foi, então, recomendado mais um período de 15 dias de tratamento, de modo semelhante ao primeiro. Trinta e nove dias após o diagnóstico inicial não foi mais isolado P. zopfii do leite dos quartos afetados e a produção retornou a níveis semelhantes de antes da infecção. Amostras de leite naturalmente infectadas foram congelados a -20°C. Células viáveis de P. zopfii foram recuperadas de amostras mantidas até 38 dias nestas condições.A clinical case of mastitis caused by Prototheca zopfii in a cow from a dairy herd located in the region of Zona da

Fiebre Tifoidea Diagnóstico por pruebas inmunoenzimáticas: Elisa

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Miguel Guzmán

1981-06-01

Full Text Available Este trabajo describe el desarrollo y normalización de una técnica inmunoenzimática para el diagnóstico indirecto de la Fiebre Tifoidea. El método permite un análisis simple y objetivo de los resultados. La reacción enzimática es proporcional a la concentración de anticuerpos en el suero contra el antígeno somático-0, por tanto, el método es cuantitativo. Por lo demás, la técnica tiene un alto grado de especificidad para Salmonella typhi, ya que los sueros de pacientes con Salmonelosis causada por Salmonella enteritidis serotipos paratyphi A, B y typhimurium dieron resultados negativos, en forma similar a los presentados por el grupo control antes de la vacunación específica. Los resultados obtenidos con esta técnica permitieron definir el nivel de anticuerpos que puede presentar una población control supuestamente sana frente a los niveles inducidos por la enfermedad. Los resultados postvacunales en el grupo control mostraron títulos sorprendentemente bajos; un análisis de este fenómeno se presentó en forma amplia. Igualmente se proponen futuras investigaciones sobre este campo.

Estudo do papel do gene Rv1358 no estabelecimento e maturação dos biofilmes formados por bactérias do complexo Mycobacterium Tuberculosis

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Vaz, Marta Helena Melo de Campos e Cunha

2016-01-01

Tese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2016 A tuberculose é uma doença infeciosa de etiologia bacteriana, com uma incidência anual média superior a 9 milhões de casos e tendo sido associada à morte de 1.5 milhões de indivíduos só em 2014. Dentro do género Mycobacterium, o complexo Mycobacterium tuberculosis (MTC) engloba várias espécies patogénicas, fenotipica e genotipicamente relacionadas, nomeadamente Mycobacterium tuberculosis, o...

The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae.

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Phelan, Jody; Maitra, Arundhati; McNerney, Ruth; Nair, Mridul; Gupta, Antima; Coll, Francesc; Pain, Arnab; Bhakta, Sanjib; Clark, Taane G

2015-09-01

Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae. Copyright © 2015 Asian-African Society for Mycobacteriology. Published by Elsevier Ltd. All rights reserved.

Análisis Ultraestructural del Efecto Neuroprotector de la Melatonina sobre las lesiones del Cortex Cerebeloso del Embrión de Pollo inducidas por Glutamato Monosódico.

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García de la Oliva, Alejandro

2017-01-01

En el presente estudio analizamos el efecto neuroprotector que la administración exógena de la melatonina ejerce sobre las lesiones neurodegenerativas de la corteza cerebelosa del embrión de pollo causadas por excitotoxicidad glutamatérgica. Para ello hemos diseñado un modelo experimental que nos permite: por un lado, evaluar y seguir la evolución de las lesiones que sobre el desarrollo de la corteza cerebelosa produce la administración de gl...

Osteomalacia inducida por tumor: hemangiopericitoma rinosinusal Tumor-induced osteomalacia: rhinosinusal hemangiopericytoma

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Enriqueta M. Serafini

2013-02-01

Full Text Available La osteomalacia inducida por tumor es una rara enfermedad del metabolismo óseo caracterizada por el aumento en la excreción de fosfato a nivel renal seguido de hipofosfatemia. Es causada por agentes fosfatúricos producidos por determinados tumores. La resección total del tumor resulta en la completa reversión de las anormalidades bioquímicas, la desaparición de las manifestaciones clínicas y los hallazgos en los estudios por imágenes. Presentamos el caso de un varón de 61 años con cuadro clínico y laboratorio compatibles con osteomalacia oncogénica inducida por tumor mesenquimático de localización rinosinusal. En nuestro caso el diagnóstico histológico correspondió a una neoplasia de tipo vascular: hemangiopericitoma.Tumor-induced osteomalacia is a rare disease of bone metabolism. The characteristic of this disease is an increase in phosphate excretion followed by hypophosphatemia, due to phosphaturic agents produced by different types of tumors. Tumor resection results in complete resolution of clinical, biochemical and radiological abnormalities. We present the case of a 61 year old man with signs, symptoms and laboratory findings consistent with oncogenic osteomalacia due to a rhino-sinusal mesenchymal tumor. The histological diagnosis showed a vascular neoplasm: hemangiopericytoma.

[Identification and drug susceptibility testing of Mycobacterium thermoresistibile and Mycobacterium elephantis isolated from a cow with mastitis].

Science.gov (United States)

Li, W B; Ji, L Y; Xu, D L; Liu, H C; Zhao, X Q; Wu, Y M; Wan, K L

2018-05-10

Objective: To understand the etiological characteristics and drug susceptibility of Mycobacterium thermoresistibile and Mycobacterium elephantis isolated from a cow with mastitis and provide evidence for the prevention and control of infectious mastitis in cows. Methods: The milk sample was collected from a cow with mastitis, which was pretreated with 4 % NaOH and inoculated with L-J medium for Mycobacterium isolation. The positive cultures were initially identified by acid-fast staining and multi-loci PCR, then Mycobacterium species was identified by the multiple loci sequence analysis (MLSA) with 16S rRNA , hsp65 , ITS and SodA genes. The drug sensitivity of the isolates to 27 antibiotics was tested by alamar blue assay. Results: Two anti-acid stain positive strains were isolated from the milk of a cow with mastitis, which were identified as non- tuberculosis mycobacterium by multi-loci PCR, and multi-loci nucleic acid sequence analysis indicated that one strain was Mycobacterium thermoresistibile and another one was Mycobacterium elephantis . The results of the drug susceptibility test showed that the two strains were resistant to most antibiotics, including rifampicin and isoniazid, but they were sensitive to amikacin, moxifloxacin, levofloxacin, ethambutol, streptomycin, tobramycin, ciprofloxacin and linezolid. Conclusions: Mycobacterium thermoresistibile and Mycobacterium elephantis were isolated in a cow with mastitis and the drug susceptibility spectrum of the pathogens were unique. The results of the study can be used as reference for the prevention and control the infection in cows.

Sea urchin granuloma Granulomas por ouriços-do-mar

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André Luiz Rossetto

2006-10-01

Full Text Available Injuries caused by venomous and poisonous aquatic animals may provoke important morbidity in humans. The phylum Echinoderma include more than 6000 species of starfish, sea urchins, sand dollars, and sea cucumbers some of which have been found responsible for injuries to humans. Initial injuries by sea urchins are associated with trauma and envenomation, but later effects can be observed. Sea urchin granuloma is a chronic granulomatous skin disease caused by frequent and successive penetration of sea urchin spines which have not been removed from wounds. The authors report a typical case of sea urchin granuloma in a fisherman and its therapeutic implications.Os acidentes por animais aquáticos traumatizantes e venenosos podem provocar morbidez importante em humanos. Equinodermos marinhos incluem mais de 6000 espécies de estrelas-do-mar, ouriços-do-mar, "bolachas-de-praia" e pepinos-do-mar. Vários equinodermos têm sido responsabilizados por acidentes em humanos. Granulomas por ouriço-do-mar são lesões de caráter granulomatoso, crônicas, causada por acidentes com espículas de ouriço-do-mar. Os autores relatam um caso típico de granulomas por ouriço-do-mar ocorrido em um pescador e enfatizam as implicações terapêuticas aplicadas.

Diagnostico por PCR del complejo Sigatoka en Colombia.

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Romero Magally

1999-06-01

Full Text Available Las Sigatokas negra y amarilla son enfermedades causadas por Mycosphaerella fijiensis y Mycosphaerella musicola respectivamente. Estas dos especies de hongos estrechamente relacionadas y morfológicamente similares, causan necrosis severa en las hojas de banano y plátano disminuyendo el área de tejido fotosintético y acelerando la maduración del fruto. Las enfermedades producidas por estos hongos afectan la mayoría de las áreas cultivadas de banano y plátano a nivel mundial, generando un gran problema económico y ambiental. Los síntomas que produce cada uno de estos patógenos en el cultivo son similares, por lo que su correcta identificación en campo se hace difícil. Con el fin de hacer una identificación precisa de cada uno de ellos decidimos estandarizar una prueba diagnóstica basada en la reacción en cadena de la polimerasa (PCR utilizando dos oligonucleótidos de 21 bases (MF 137 y MM 137 específicos para cada una de las especies M. fijiensis y M. musicola respectivamente. MF 137 y MM 137 codifican regiones variables identificadas en las secuencias interespaciadoras (ITS del DNA ribosomal.

Mycobacterium talmoniae sp. nov., a slowly growing mycobacterium isolated from human respiratory samples.

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Davidson, Rebecca M; DeGroote, Mary Ann; Marola, Jamie L; Buss, Sarah; Jones, Victoria; McNeil, Michael R; Freifeld, Alison G; Elaine Epperson, L; Hasan, Nabeeh A; Jackson, Mary; Iwen, Peter C; Salfinger, Max; Strong, Michael

2017-08-01

A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812T (=ATCC BAA-2683T=DSM 46873T).

Surtos de dermatite ulcerativa causados por espinhos de Mimosa setosa, M. debilis e M. pudica (Fabaceae em equinos

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Saulo A. Caldas

Full Text Available RESUMO: Descrevem-se, na pele de equídeos, lesões de natureza mecânico-traumática causadas por espinhos de Mimosa spp. Dentre as três espécies da planta identificadas como responsáveis pelas lesões, M. setosa estava presente em maior quantidade (80% e M. debilis e M. pudica encontravam-se em menor proporção na pastagem. Ocorreram três surtos de dermatite ulcerativa em períodos chuvosos de abril a maio de 2013, dezembro de 2013 a fevereiro de 2014 e abril a maio deste mesmo ano. Vinte e cinco equinos do Setor de Reprodução Animal da Universidade Federal Rural do Rio de Janeiro apresentaram, na pele, lesões ulcerativas com contornos irregulares, exsudato hemorrágico, recobertas por crostas. As lesões localizavam-se sobretudo nas regiões de quartela, boleto, articulação escapulo-umeral, lábios superior e inferior, focinho, narinas, bochechas e chanfro. Sete animais foram biopsiados e o exame histopatológico revelou ulceração da epiderme e infiltrado inflamatório constituído por macrófagos e neutrófilos, delimitado por tecido de granulação subjacente. Em alguns casos, foram observados microespículos das referidas plantas (tricomas hirsutos em meio à reação inflamatória. O diagnóstico de dermatite cutânea causada pela ação traumática da planta baseou-se na presença de Mimosa spp. na pastagem, nas características e localização das lesões na pele dos equinos, nos achados histopatológicos e na recuperação após a retirada dos animais do pasto.

Aspectos anatomopatológicos em cães naturalmente infectados por Hepatozoon canis

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Lima, Pâmela A.; Barçante, Joziana M.P.; Boeloni, Jankerle N.; Bezerra Júnior, Pedro S.; Wouters, Flademir; Wouters, Angélica T.; Varaschin, Mary S.; Seixas, Josilene N.

2017-01-01

RESUMO: A hepatozoonose canina é causada principalmente pelos protozoários Hepatozoon canis e H. americanum, transmitida por ingestão de carrapatos parasitados. Os sinais clínicos podem ser inespecíficos ou de difícil reconhecimento, pois geralmente ocorre associada a outras doenças. No Brasil, o parasito, e a doença, já foram identificados em vários Estados, no entanto pouco se sabe sobre as alterações clínicas e anátomo-patológicas decorrentes da infecção. O presente trabalho relata cinco c...

Surto de abscessos cutâneos persistentes por Mycobacterium chelonae pós-mesoterapia, Lima, Peru

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Munayco, César V; Grijalva, Carlos G; Culqui, Dante R; Bolarte, José L; Suárez-Ognio, Luis A; Quispe, Neyda; Calderon, Roger; Ascencios, Luis; Del Solar, Manuel; Salomón, Martín; Bravo, Francisco; Gotuzzo, Eduardo

2008-01-01

Outbreaks of rapidly growing mycobacteria have been occasionally described. The article reports an outbreak of cutaneous abscesses due to Mycobacterium chelonae following mesotherapy in Lima, Peru. From December 2004 through January 2005, 35 subjects who had participated in mesotherapy training sessions presented with persistent cutaneous abscesses. Thirteen (37%) of these suspected cases consented to underwent clinical examination. Skin punch-biopsies were collected from suspicious lesions a...

Controle biológico da podridão radicular (Pythium aphanidermatum e promoção de crescimento por Pseudomonas chlororaphis 63-28 e Bacillus subtilis GB03 em alface hidropônica

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Élida Barbosa Corrêa

2010-12-01

Full Text Available Podridões radiculares causadas por espécies de Pythium são um importante problema em cultivos hidropônicos. Sintomas de subdesenvolvimento são observados nas plantas parasitadas pelo patógeno, sendo muitas vezes não diagnosticados pelo produtor. O objetivo do trabalho foi avaliar o controle biológico da podridão radicular causada por Pythium aphanidermatum e a promoção de crescimento por Pseudomonas chlororaphis 63-28 e Bacillus subtilis GB03, reconhecidos agentes de controle biológico de doenças de plantas. A inoculação das plantas com P. aphanidermatum ocasionou o subdesenvolvimento, sendo essa diminuição de 20%. A adição dos agentes de biocontrole na solução nutritiva teve um efeito positivo no aumento da massa (6% a 13%, no número de folhas (4% a 7% e no teor de clorofila (3% das plantas de alface. Entretanto, maiores estudos devem ser realizados para melhorar a capacidade de controle da doença e de promoção de crescimento pelos agentes de biocontrole estudados no cultivo de alface hidropônica.

Caracterización de las alteraciones de los monocitos en las diferentes formas clínicas de la tuberculosis

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Mauricio Rojas

2003-01-01

Full Text Available En el ámbito mundial, la tuberculosis (TB es la enfermedad
infecciosa asociada con la mayor mortalidad; es causada por
Mycobacterium tuberculosis (MTB. Los monocitos/macrófagos juegan un papel fundamental en la respuesta inmune antimicobacteriana: ellos pueden controlar la infección inicial mediante la respuesta innata, desencadenan la respuesta inmune adquirida por medio de la presentación de antígenos micobacterianos a las células T, y adquieren capacidades microbicidas como consecuencia de la activación mediada por las células T. Se sabe que una minoría de individuos infectados (5-10% desarrolla TB y que los pacientes con TB presentan alteraciones en su respuesta inmune específica, las cuales pueden variar dependiendo de la extensión de la infección, y que pueden incluir tanto las funciones de los monocito/macrófagos  presentación antigénica y apoptosis y de las células T (producción de citoquinas proinflamatorias o antiinflamatorias (1.

Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex.

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Bouam, Amar; Heidarieh, Parvin; Shahraki, Abodolrazagh Hashemi; Pourahmad, Fazel; Mirsaeidi, Mehdi; Hashemzadeh, Mohamad; Baptiste, Emeline; Armstrong, Nicholas; Levasseur, Anthony; Robert, Catherine; Drancourt, Michel

2018-03-07

Four slowly growing mycobacteria isolates were isolated from the respiratory tract and soft tissue biopsies collected in four unrelated patients in Iran. Conventional phenotypic tests indicated that these four isolates were identical to Mycobacterium lentiflavum while 16S rRNA gene sequencing yielded a unique sequence separated from that of M. lentiflavum. One representative strain AFP-003 T was characterized as comprising a 6,121,237-bp chromosome (66.24% guanosine-cytosine content) encoding for 5,758 protein-coding genes, 50 tRNA and one complete rRNA operon. A total of 2,876 proteins were found to be associated with the mobilome, including 195 phage proteins. A total of 1,235 proteins were found to be associated with virulence and 96 with toxin/antitoxin systems. The genome of AFP-003 T has the genetic potential to produce secondary metabolites, with 39 genes found to be associated with polyketide synthases and non-ribosomal peptide syntases and 11 genes encoding for bacteriocins. Two regions encoding putative prophages and three OriC regions separated by the dnaA gene were predicted. Strain AFP-003 T genome exhibits 86% average nucleotide identity with Mycobacterium genavense genome. Genetic and genomic data indicate that strain AFP-003 T is representative of a novel Mycobacterium species that we named Mycobacterium ahvazicum, the nineteenth species of the expanding Mycobacterium simiae complex.

Mycobacterium arupense, Mycobacterium heraklionense, and a Newly Proposed Species, "Mycobacterium virginiense" sp. nov., but Not Mycobacterium nonchromogenicum, as Species of the Mycobacterium terrae Complex Causing Tenosynovitis and Osteomyelitis.

Science.gov (United States)

Vasireddy, Ravikiran; Vasireddy, Sruthi; Brown-Elliott, Barbara A; Wengenack, Nancy L; Eke, Uzoamaka A; Benwill, Jeana L; Turenne, Christine; Wallace, Richard J

2016-05-01

Mycobacterium terrae complex has been recognized as a cause of tenosynovitis, with M. terrae and Mycobacterium nonchromogenicum reported as the primary etiologic pathogens. The molecular taxonomy of the M. terrae complex causing tenosynovitis has not been established despite approximately 50 previously reported cases. We evaluated 26 isolates of the M. terrae complex associated with tenosynovitis or osteomyelitis recovered between 1984 and 2014 from 13 states, including 5 isolates reported in 1991 as M. nonchromogenicum by nonmolecular methods. The isolates belonged to three validated species, one new proposed species, and two novel related strains. The majority of isolates (20/26, or 77%) belonged to two recently described species: Mycobacterium arupense (10 isolates, or 38%) and Mycobacterium heraklionense (10 isolates, or 38%). Three isolates (12%) had 100% sequence identity to each other by 16S rRNA and 99.3 to 100% identity by rpoB gene region V sequencing and represent a previously undescribed species within the M. terrae complex. There were no isolates of M. terrae or M. nonchromogenicum, including among the five isolates reported in 1991. The 26 isolates were susceptible to clarithromycin (100%), rifabutin (100%), ethambutol (92%), and sulfamethoxazole or trimethoprim-sulfamethoxazole (70%). The current study suggests that M. arupense, M. heraklionense, and a newly proposed species ("M. virginiense" sp. nov.; proposed type strain MO-233 [DSM 100883, CIP 110918]) within the M. terrae complex are the major causes of tenosynovitis and osteomyelitis in the United States, with little change over 20 years. Species identification within this complex requires sequencing methods. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Intoxicação por Portulaca elatior (Portulacaceae em caprinos

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Glauco J.N. Galiza

2011-06-01

Full Text Available Um surto de intoxicação espontânea por Portulaca elatior foi diagnosticado no município de Aroeiras, Paraíba, na época das chuvas. Entre os meses de fevereiro e abril, em um rebanho de 316 caprinos, 16 adoeceram, sendo 15 de um lote de 16 caprinos que tinham sido introduzidos recentemente na fazenda e um animal do rebanho já existente na fazenda. Os caprinos apresentaram intensa salivação, relutância em se movimentar, tremores de lábios, marcha incoordenada, diarreia, timpanismo, dor abdominal, gemidos, desidratação e berros constantes. A evolução da enfermidade variou de 2 a 48 horas; 13 caprinos morreram e três se recuperaram. As lesões macroscópicas caracterizavam-se por mucosa ruminal e do retículo avermelhadas, serosa do rúmen hemorrágica, mucosa abomasal avermelhada com áreas ulceradas e mucosa do intestino delgado com equimoses. No exame histopatológico observou-se degeneração e necrose das células epiteliais dos pré-estômagos com formação de vesículas e infiltrado inflamatório neutrofílico. Suspeita de ter causado o surto, Portulaca elatior foi administrada a três caprinos nas doses de 40, 20 e 10 gramas de planta fresca por kg de peso animal (g/kg. Esses caprinos apresentaram sinais semelhantes aos casos espontâneos. Os animais que ingeriram 20 e 40g/kg morreram e o que ingeriu 10g/kg se recuperou. Microscopicamente foram observadas lesões do sistema digestivo semelhantes às dos casos espontâneos. A reprodução experimental da intoxicação, com sinais clínicos e lesões histológicas semelhantes às dos casos espontâneos, comprova que a doença foi causada por Portulaca elatior. P. elatior apresentou níveis de oxalatos de 6,37% e outra planta, identificada como Blutaparon vermiculare e que ocorria em grande quantidade nas pastagens, apresentou níveis de oxalatos de 5,29%. B. vermiculare foi administrada experimentalmente a um caprino na dose de 40g/kg, sem que o animal apresentasse sinais cl

Avaliação sorológica e de fatores de risco para a infecção por Mycobacterium avium subsp. paratuberculosis em rebanhos leiteiros da Microrregião de Garanhuns, Pernambuco Serological evaluation and risk factors for Mycobacterium avium subsp. paratuberculosis infection in dairy herds of Microregion Garanhuns, Pernambuco

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Luenda de M. e Sá

2013-03-01

Full Text Available Objetivou-se com esse trabalho realizar um inquérito epidemiológico da infecção por Mycobacterium avium subsp. paratuberculosis (MAP em bovinos leiteiros da microrregião de Garanhuns, Pernambuco, Brasil. Para este estudo foram coletadas amostras sanguíneas de 408 animais, provenientes de 19 rebanhos localizados em 15 municípios. O exame sorológico foi realizado por Ensaio Imunoenzimático (ELISA indireto para detecção de anticorpos frente ao MAP. Em todas as propriedades, foi aplicado um questionário investigativo para análise dos fatores de risco, e as coordenadas geográficas coletadas por um aparelho de Global Position System (GPS para realização da distribuição espacial. A prevalência da infecção por MAP foi de 2,7% (11/408; I.C. 1,4-4,9. O número de focos foi 47,4% (9/19. Na análise de regressão logística foi identificado como fator de risco a taxa anual de nascimentos superior a 51 bezerros/ano (OR 3,8; I.C. 1,1-13,1. Desta forma, conclui-se que a infecção por MAP encontra-se presente nos rebanhos bovinos leiteiros da microrregião estudada e que medidas de controle baseadas nos fatores de risco identificados devem ser implementadas com o objetivo de reduzir o número de focos da infecção.The present study aimed to conduct an epidemiological investigation of Mycobacterium avium subsp. paratuberculosis (MAP infection in dairy cattle of the Garanhuns microregion, in Pernambuco, Brazil. Blood samples were collected from 408 animals from 19 herds located in 15 cities. Serological tests were performed by indirect immunoenzymatic assay (ELISA for antibodies against MAP. In all farms, a questionnaire to investigate risk factors was used, and Global Position System (GPS receivers were used to collect geographic coordinates to show the spatial distribution of the animals. The prevalence of MAP infected cattle was 2.7% (11/408; I.C. 1.4-4.9. The rate of infection was 47.4% (9/19. An annual birth rate over 51 calves

LIMITAÇÕES E CONSEQUÊNCIAS NA VIDA DO TRABALHADOR OCASIONADAS POR DOENÇAS RELACIONADAS AO TRABALHO

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Bruna Caroline Rodrigues

2013-01-01

Full Text Available El objetivo fue comprobar en la literatura el impacto de las enfermedades profesionales en la vida de los trabajadores y analizar las contribuciones de los estudios para la ciencia de enfermería, en especial para enfermería del trabajo. Revisión integradora con la siguiente pregunta: cuáles son las limitaciones y consecuencias causadas por trastornos de trauma acumulativo en la vida de los trabajadores, en la literatura científica de enfermería en los últimos cinco años (2006 a 2010. Se utilizaron como descriptores: Salud Ocupacional y Salud Ocupacional del Trabajador. Fueron seleccionados 14 trabajos que se agruparon según objetivos comunes, principales limitaciones y consecuencias causadas e informaciones que se pueden traducir en contribuciones para la Enfermería del Trabajo. La producción científica sobre el tema trae pocas contribuciones efectivas, y que se necesitan más estudios para subsidiar a las estrategias de atención dirigidas a promover la salud y la calidad de vida de estos trabajadores.

DETECÇÃO DO COMPLEXO Mycobacterium tuberculosis NO LEITE PELA REAÇÃO EM CADEIA DA POLIMERASE SEGUIDA DE ANÁLISE DE RESTRIÇÃO DO FRAGMENTO AMPLIFICADO (PRA DETECTION OF Mycobacterium tuberculosis COMPLEX BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORFISM ANALYSIS OF THE HSP65 GENE

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Joab Trajano Silva

2008-12-01

Full Text Available Mycobacterium bovis é membro do complexo Mycobacterium tuberculosis (MTBC, grupo este composto por espécies com grande homologia genética. É o agente etiológico da tuberculose bovina, importante zoonose transmissível ao homem, principalmente através da inalação do bacilo e/ou pelo consumo de leite e derivados não-pasteurizados provenientes de vacas tuberculosas. O objetivo deste estudo foi padronizar a identificação de micobactérias do complexo M. tuberculosis presentes no leite, por metodologia molecular. Fez-se a extração de DNA diretamente do leite contaminado e realizou-se a identificação molecular pela reação em cadeia da polimerase seguida de análise de restrição do fragmento amplificado (PRA. Utilizaram-se inhagens de referência e leite cru artificialmente contaminado com M. bovis IP. Um fragmento de 441pb do gene hsp65 foi amplificado, tratado com BstEII e HaeIII e empregou-se o perfil de restrição enzimática obtido para identificar o complexo M. tuberculosis no leite. Com a PRA foi possível detectar com especificidade e sensibilidade a presença de M. bovis em até 10 UFC/mL de leite. A metodologia padronizada poderá auxiliar os métodos microbiológicos e bioquímicos tradicionalmente usados na identificação do bacilo em alimentos suspeitos de contaminação, como, por exemplo, o leite proveniente de animais suspeitos de infecção por M. bovis.

Palavras-chaves: Análise de perfil de restrição enzimática (PRA, complexo Mycobacterium tuberculosis, leite, Mycobacterium bovis, limite de detecção (PCR. Mycobacterium bovis is a member of the M. tuberculosis complex, a group composed by species with high genetic homology. The pathogen is the etiological agent of bovine tuberculosis, an important zoonosis that is mainly transmitted by inhalation of infectious droplet nuclei or by ingestion of milk and crude milk derivative products from tuberculosis cows. The definitive identification of M. bovis

Mycobacterium arupense, Mycobacterium heraklionense, and a Newly Proposed Species, “Mycobacterium virginiense” sp. nov., but Not Mycobacterium nonchromogenicum, as Species of the Mycobacterium terrae Complex Causing Tenosynovitis and Osteomyelitis

Science.gov (United States)

Vasireddy, Sruthi; Brown-Elliott, Barbara A.; Wengenack, Nancy L.; Eke, Uzoamaka A.; Benwill, Jeana L.; Turenne, Christine; Wallace, Richard J.

2016-01-01

Mycobacterium terrae complex has been recognized as a cause of tenosynovitis, with M. terrae and Mycobacterium nonchromogenicum reported as the primary etiologic pathogens. The molecular taxonomy of the M. terrae complex causing tenosynovitis has not been established despite approximately 50 previously reported cases. We evaluated 26 isolates of the M. terrae complex associated with tenosynovitis or osteomyelitis recovered between 1984 and 2014 from 13 states, including 5 isolates reported in 1991 as M. nonchromogenicum by nonmolecular methods. The isolates belonged to three validated species, one new proposed species, and two novel related strains. The majority of isolates (20/26, or 77%) belonged to two recently described species: Mycobacterium arupense (10 isolates, or 38%) and Mycobacterium heraklionense (10 isolates, or 38%). Three isolates (12%) had 100% sequence identity to each other by 16S rRNA and 99.3 to 100% identity by rpoB gene region V sequencing and represent a previously undescribed species within the M. terrae complex. There were no isolates of M. terrae or M. nonchromogenicum, including among the five isolates reported in 1991. The 26 isolates were susceptible to clarithromycin (100%), rifabutin (100%), ethambutol (92%), and sulfamethoxazole or trimethoprim-sulfamethoxazole (70%). The current study suggests that M. arupense, M. heraklionense, and a newly proposed species (“M. virginiense” sp. nov.; proposed type strain MO-233 [DSM 100883, CIP 110918]) within the M. terrae complex are the major causes of tenosynovitis and osteomyelitis in the United States, with little change over 20 years. Species identification within this complex requires sequencing methods. PMID:26962085

Inactivation of Mycobacterium paratuberculosis and Mycobacterium tuberculosis in fresh soft cheese by gamma radiation

International Nuclear Information System (INIS)

Badr, Hesham M.

2011-01-01

The effectiveness of gamma irradiation on the inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese that prepared from artificially inoculated milk samples was studied. Irradiation at dose of 2 kGy was sufficient for the complete inactivation of these mycobacteria as they were not detected in the treated samples during storage at 4±1 o C for 15 days. Moreover, irradiation of cheese samples, that were prepared from un-inoculated milk, at this effective dose had no significant effects on their gross composition and contents from riboflavin, niacin and pantothenic acid, while significant decreases in vitamin A and thiamin were observed. In addition, irradiation of cheese samples had no significant effects on their pH and nitrogen fractions contents, except for the contents of ammonia, which showed a slight, but significant, increases due to irradiation. The analysis of cheese fats indicated that irradiation treatment induced significant increase in their oxidation parameters and contents from free fatty acids; however, the observed increases were relatively low. On the other hand, irradiation of cheese samples induced no significant alterations on their sensory properties. Thus, irradiation dose of 2 kGy can be effectively applied to ensure the safety of soft cheese with regards to these harmful mycobacteria. - Highlights: → We examined the effectiveness of gamma irradiation on inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese. → Irradiation at dose of 2 kGy was sufficient for complete inactivation of these mycobacteria. → Irradiation of cheese samples induced no significant alterations on their sensory properties.

Inactivation of Mycobacterium paratuberculosis and Mycobacterium tuberculosis in fresh soft cheese by gamma radiation

Energy Technology Data Exchange (ETDEWEB)

Badr, Hesham M., E-mail: heshambadr_aea@yahoo.co.uk [Atomic Energy Authority, Nuclear Research Center, Abou Zaabal, P.O. Box 13759 Cairo (Egypt)

2011-11-15

The effectiveness of gamma irradiation on the inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese that prepared from artificially inoculated milk samples was studied. Irradiation at dose of 2 kGy was sufficient for the complete inactivation of these mycobacteria as they were not detected in the treated samples during storage at 4{+-}1 {sup o}C for 15 days. Moreover, irradiation of cheese samples, that were prepared from un-inoculated milk, at this effective dose had no significant effects on their gross composition and contents from riboflavin, niacin and pantothenic acid, while significant decreases in vitamin A and thiamin were observed. In addition, irradiation of cheese samples had no significant effects on their pH and nitrogen fractions contents, except for the contents of ammonia, which showed a slight, but significant, increases due to irradiation. The analysis of cheese fats indicated that irradiation treatment induced significant increase in their oxidation parameters and contents from free fatty acids; however, the observed increases were relatively low. On the other hand, irradiation of cheese samples induced no significant alterations on their sensory properties. Thus, irradiation dose of 2 kGy can be effectively applied to ensure the safety of soft cheese with regards to these harmful mycobacteria. - Highlights: > We examined the effectiveness of gamma irradiation on inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese. > Irradiation at dose of 2 kGy was sufficient for complete inactivation of these mycobacteria. > Irradiation of cheese samples induced no significant alterations on their sensory properties.

Microaerobic growth and anaerobic survival of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum.

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Lewis, Amy Herndon; Falkinham, Joseph O

2015-03-01

Representative strains of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum (MAIS) grew at equal rates in laboratory medium at 21% (air) and 12% oxygen. Growth in 6% oxygen proceeded at a 1.4-1.8-fold lower rate. Colony formation was the same at 21% (air) and 6% oxygen. The MAIS strains survived rapid shifts from aerobic to anaerobic conditions as measured by two experimental approaches (Falkinham (1996) [1]). MAIS cells grown aerobically to log phase in broth were diluted, spread on agar medium, and incubated anaerobically for up to 20 days at 37°C. Although no colonies formed anaerobically, upon transfer to aerobic conditions, greater than 25% of the colony forming units (CFU) survived after 20 days of anaerobic incubation (Prince et al. (1989) [2]). MAIS cells grown in broth aerobically to log phase were sealed and vigorous agitation led to oxygen depletion (Wayne model). After 12 days anaerobic incubation, M. avium and M. scrofulaceum survival were high (>50%), while M. intracellulare survival was lower (22%). M. avium cells shifted to anaerobiosis in broth had increased levels of glycine dehydrogenase and isocitrate lyase. Growth of MAIS strains at low oxygen levels and their survival following a rapid shift to anaerobiosis is consistent with their presence in environments with fluctuating oxygen levels. Copyright © 2015 Asian African Society for Mycobacteriology. Published by Elsevier Ltd. All rights reserved.

Microaerobic growth and anaerobic survival of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum

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Amy Herndon Lewis

2015-01-01

Full Text Available Representative strains of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum (MAIS grew at equal rates in laboratory medium at 21% (air and 12% oxygen. Growth in 6% oxygen proceeded at a 1.4–1.8-fold lower rate. Colony formation was the same at 21% (air and 6% oxygen. The MAIS strains survived rapid shifts from aerobic to anaerobic conditions as measured by two experimental approaches (Falkinham (1996 [1]. MAIS cells grown aerobically to log phase in broth were diluted, spread on agar medium, and incubated anaerobically for up to 20 days at 37 °C. Although no colonies formed anaerobically, upon transfer to aerobic conditions, greater than 25% of the colony forming units (CFU survived after 20 days of anaerobic incubation (Prince et al. (1989 [2]. MAIS cells grown in broth aerobically to log phase were sealed and vigorous agitation led to oxygen depletion (Wayne model. After 12 days anaerobic incubation, M. avium and M. scrofulaceum survival were high (>50%, while M. intracellulare survival was lower (22%. M. avium cells shifted to anaerobiosis in broth had increased levels of glycine dehydrogenase and isocitrate lyase. Growth of MAIS strains at low oxygen levels and their survival following a rapid shift to anaerobiosis is consistent with their presence in environments with fluctuating oxygen levels.

Characterization of a Mycobacterium leprae antigen related to the secreted Mycobacterium tuberculosis protein MPT32

NARCIS (Netherlands)

Wieles, B.; van Agterveld, M.; Janson, A.; Clark-Curtiss, J.; Rinke de Wit, T.; Harboe, M.; Thole, J.

1994-01-01

Secreted proteins may serve as major targets in the immune response to mycobacteria. To identify potentially secreted Mycobacterium leprae antigens, antisera specific for culture filtrate proteins of Mycobacterium tuberculosis were used to screen a panel of recombinant antigens selected previously

Distribución parcial de Paragonimus (Digenea: Troglotrematidae) en Antioquia, por presencia de metacercarias en cangrejos dulciacuícolas

OpenAIRE

Luz Elena Velásquez; Mónica Uruburu; Mabel Granada

2008-01-01

Introducción. La paragonimosis, o distomatosis pulmonar, es una enfermedad con sintomatología similar a la observada en la tuberculosis. Es causada por parásitos del género Paragonimus (Digenea: Troglotrematidae). Las personas se infectan al consumir cangrejos crudos o mal cocidos, con metacercarias del parásito. El primer foco de paragonimosis humana en Colombia se registró durante 1995 en Urrao, Antioquia, donde se hallaron dos especies de cangrejos que hospedaban el parásito. En el 2005 se...

Coexistência de infeções oportunistas pulmonares num doente com infeção por vírus da imunodeficiência humana e uma forma persistente de pneumonia por Pneumocystis jirovecii: caso clínico

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D. Ponces Bento

2013-05-01

Full Text Available Resumo: Como é sabido, nos doentes com infeção por vírus da imunodeficiência humana (VIH existe um alto risco de ocorrência de infeções oportunistas (IO, tais como as infeções por Pneumocystis jirovecii, um agente patogénico com distribuição mundial, que provoca pneumonia intersticial (PPc. Apresentamos um caso de um doente recém-diagnosticado com infeção por VIH-1 e múltiplas IO pulmonares, incluindo uma forma persistente de PPc, aspergilose invasiva (AI, e infeções por citomegalovírus e por Mycobacterium xenopi. Descrevemos a combinação de fatores cruciais para a recuperação do doente, que incluíram a obtenção de dados laboratoriais, intervenção cirúrgica e múltipla terapêutica antimicrobiana. Abstract: It is well established that HIV patients are at high risk of opportunistic infections (OI, like the ones caused by Pneumocystis jirovecii, a worldwide pathogen implicated in interstitial pneumonia (PcP. We present a case of a newly diagnosed HIV-1 patient with multiple OI, including a persistent form of PcP, an invasive aspergillosis (IA, cytomegalovirus and Mycobacterium xenopi lung infection. We describe the combination of laboratorial screening, surgery and antimicrobial therapy that were crucial for patient recovery. Palavras-chave: Vírus da imunodeficiência humana, Pneumonia por Pneumocystis jirovecii, Infeções oportunistas, Keywords: Human immunodeficiency virus, Pneumocystis jirovecii pneumonia, Opportunistic lung infections

Efeitos da rottlerin na esquizogonia eritrocitária de Plasmodium falciparum e implementação e avaliação de teste in vitro por fluorescência de atividade antiplasmodial

OpenAIRE

Cordeiro, Thuany de Moura

2014-01-01

A malária é uma doença infecciosa causada por protozoários Plasmodium spp. O P. falciparum é considerado o mais severo por ser o responsável pela maioria dos casos de morte causados pela doença. Devido ao rápido surgimento de cepas de P. falciparum resistentes às drogas antimalariais dá-se a importância de realizar um screening de compostos da biodiversidade, além de elucidar os mecanismos de ação de substâncias com comprovada ação antiplasmodial, como por exemplo, a rottlerin, um inibidor da...

Efectos de la administración de un inhibidor de la recaptación de serotonina, Sertralina, sobre los cambios inducidos por el estrés prenatal

OpenAIRE

Figueiredo, Inês Pereira de

2016-01-01

[ES] En las postrimerías del siglo pasado, se constató una vez más que las alteraciones causadas por estrés y ansiedad en la sociedad moderna, se encuentran entre los problemas de salud más comunes. Según la Organización Mundial de Salud (OMS), al menos el 25% de los adultos sufrirá alguna forma de trastornos por ansiedad en algún momento de su vida (Gordon y Hen, 2004; Anacker y cols., 2010), siendo las mujeres más susceptibles a este tipo de trastornos (Olff y cols., 2007). ...

Comparación de las técnicas Kato-Katz, TSET y TSR en el diagnóstico de infección por Fasciola hepatica en humanos

OpenAIRE

Nelson Uribe Delgado; Raúl Fernando Sierra Balcárcel; Cindy Tatiana Espinosa González

2012-01-01

Introducción: La Fasciolosis es una enfermedad causada por el parásito Fasciola hepatica, que afecta primordialmente a bovinos, ovinos, caprinos y en algunas zonas de forma endémica a los humanos. En la investigación clínica y epidemiológica de fasciolosis humana es importante contar con técnicas coprológicas de diagnóstico de alta sensibilidad y especificidad. Objetivo: realizar la comparación de tres técnicas coprológicas para el diagnóstico de infección por Fasciola hepatica en humanos. Me...

Bacteriological diagnosis and molecular strain typing of Mycobacterium bovis and Mycobacterium caprae.

Science.gov (United States)

Gormley, E; Corner, L A L; Costello, E; Rodriguez-Campos, S

2014-10-01

The primary isolation of a Mycobacterium sp. of the Mycobacterium tuberculosis complex from an infected animal provides a definitive diagnosis of tuberculosis. However, as Mycobacterium bovis and Mycobacterium caprae are difficult to isolate, particularly for animals in the early stages of disease, success is dependent on the optimal performance of all aspects of the bacteriological process, from the initial choice of tissue samples at post-mortem examination or clinical samples, to the type of media and conditions used to cultivate the microorganism. Each step has its own performance characteristics, which can contribute to sensitivity and specificity of the procedure, and may need to be optimized in order to achieve the gold standard diagnosis. Having isolated the slow-growing mycobacteria, species identification and fine resolution strain typing are keys to understanding the epidemiology of the disease and to devise strategies to limit transmission of infection. New technologies have emerged that can now even discriminate different isolates from the same animal. In this review we highlight the key factors that contribute to the accuracy of bacteriological diagnosis of M. bovis and M. caprae, and describe the development of advanced genotyping techniques that are increasingly used in diagnostic laboratories for the purpose of supporting detailed epidemiological investigations. Copyright © 2014 Elsevier Ltd. All rights reserved.

La marea roja causada por el dinoflagelado Alexandrium tamarense en la costa Pacífica colombiana (2001

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Ingrid García-Hansen

2004-09-01

Full Text Available El 26 de marzo de 2001 se registró por primera vez en las aguas del Pacífico colombiano, en el área de la Ensenada de Tumaco, la aparición de una marea roja producida por la especie Alexandrium tamarense con valores que superaron las 7.5 x 10(6 céls l-1 , la marea se mantuvo hacia la región oceánica, derivando en sentido sur-norte por efecto de las corrientes, hasta ser vista por ultima vez cerca a la Isla Gorgona, casi tres semanas después. Un año mas tarde, en marzo de 2002, una segunda proliferación, conformada por la misma especie, se reportó cerca de Cabo Corrientes con una concentración de 1.6 x 10(6 céls l -1 . Ambos casos estuvieron relacionados con la presencia de bajas temperaturas en la superficie del mar y no estuvieron asociadas con fenómenos de intoxicación y mortalidadFrom April 26th to May 15th 2001, a large algae bloom was observed off Tumaco Bay on the Pacific coast of Colombia. This was the first harmful algae bloom (HAB reported in the region, and reached Gorgona Island, about 120 km north. Ayear later, starting March 2002, an offshore HAB developed from Cabo Corrientes North to Solano Bay. The typical abundance during the blooms reached 7.5 x 10(6 cells l-1 for the 2001 event and 1.6 x 10(6 cells l-1 for the 2002 event. During both events, low temperature and high salinity were recorded. Typical measurements in the area are 27-27.5°C and 30-31.5 psu. Values observed during the two events were 24-24.6°C and 33-34 psu; 3°C below normal and more than 2.5 psu above average values. These conditions are indicative of local upwelling processes at the time of the events. On both occasions, cells corresponding to the Alexandrium catenella/fundeyense/tamarense complex represented 99-100% of the biomass. It was difficult to differentiate the cells from A. catenella, but the presence of short chains of only 4 cells (single cells represented most of the biomass was suggestive of A. tamarense. Shape, dimensions, and

Tratamiento con caspofungina de endocarditis por Candida tropicalis resistente a fluconazol Treatment with caspofungin of Candida tropicalis endocarditis resistant to fluconazol

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Marcelo del Castillo

2004-04-01

Full Text Available Las endocarditis causadas por hongos, (Candida en particular, requieren tratamiento médico-quirúrgico, siendo la anfotericina B la droga de elección. Caspofungina es una equinocandina con gran actividad sobre Candida y Aspergillus. Se presenta un paciente con una endocarditis por Candida tropicalis resistente a fluconazol tratado con caspofungina bajo un esquema de salvataje, luego de haber presentado efectos adversos por anfotericina B. El paciente tuvo respuesta microbiológica.Fungal endocarditis, in particular due to Candida species, requires medical and surgical treatment and amphotericin B is the drug of choice. Caspofungin is an echinocandin very effective against Candida and Aspergillus. We present a patient with Candida tropicalis endocarditis, fluconazol resistant, treated with caspofungin, on a compassional basis as a result of adverse effects with amphotericin B. The patient had a microbiological response.

Lesões traumáticas de pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos

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Alessandra S. Belo Reis

2011-09-01

Full Text Available Foram realizadas pesquisas sobre a natureza e causa de lesões de pele em equídeos em uma propriedade no município de Castanhal, região Nordeste do Estado do Pará. Foram realizadas visitas técnicas, estudos epidemiológicos, coletas de sangue, biópsias de pele afetada e a inspeção da pastagem. O estudo incluiu 25 equídeos, dos quais 14 machos e 11 fêmeas, de seis meses e oito anos de idade. Os animais apresentaram lesões ulcerativas, de bordos irregulares, na cabeça (narinas, focinho, lábios superiores e inferiores e chanfro, na cavidade oral (vestíbulo bucal e gengiva e nos membros (boletos, metacarpos e metatarsos e articulação escápulo-umeral. No exame histopatológico foram observados focos de erosões cutâneas, caracterizados por perda e necrose da epiderme, com espongiose, degeneração vesicular da epiderme remanescente e leve infiltrado inflamatório na derme subjacente, constituído predominantemente por macrófagos e, em menor grau, eosinófilos. Na inspeção da pastagem, constituída de Brachiaria humidicola, foi constatada grande invasão de duas plantas providas de espinhos, Mimosa pudica e Mimosa debilis, ambas da família Leg. Mimosoideae. Concluiu-se, que as lesões de pele foram causadas pela ação traumática dos espinhos de Mimosa pudica e Mimosa debilis.

Phenotypic and genomic comparison of Mycobacterium aurum and surrogate model species to Mycobacterium tuberculosis: implications for drug discovery.

Science.gov (United States)

Namouchi, Amine; Cimino, Mena; Favre-Rochex, Sandrine; Charles, Patricia; Gicquel, Brigitte

2017-07-13

Tuberculosis (TB) is caused by Mycobacterium tuberculosis and represents one of the major challenges facing drug discovery initiatives worldwide. The considerable rise in bacterial drug resistance in recent years has led to the need of new drugs and drug regimens. Model systems are regularly used to speed-up the drug discovery process and circumvent biosafety issues associated with manipulating M. tuberculosis. These include the use of strains such as Mycobacterium smegmatis and Mycobacterium marinum that can be handled in biosafety level 2 facilities, making high-throughput screening feasible. However, each of these model species have their own limitations. We report and describe the first complete genome sequence of Mycobacterium aurum ATCC23366, an environmental mycobacterium that can also grow in the gut of humans and animals as part of the microbiota. This species shows a comparable resistance profile to that of M. tuberculosis for several anti-TB drugs. The aims of this study were to (i) determine the drug resistance profile of a recently proposed model species, Mycobacterium aurum, strain ATCC23366, for anti-TB drug discovery as well as Mycobacterium smegmatis and Mycobacterium marinum (ii) sequence and annotate the complete genome sequence of this species obtained using Pacific Bioscience technology (iii) perform comparative genomics analyses of the various surrogate strains with M. tuberculosis (iv) discuss how the choice of the surrogate model used for drug screening can affect the drug discovery process. We describe the complete genome sequence of M. aurum, a surrogate model for anti-tuberculosis drug discovery. Most of the genes already reported to be associated with drug resistance are shared between all the surrogate strains and M. tuberculosis. We consider that M. aurum might be used in high-throughput screening for tuberculosis drug discovery. We also highly recommend the use of different model species during the drug discovery screening process.

Mycobacterium fortuitum causing surgical site wound infection

International Nuclear Information System (INIS)

Kaleem, F.; Usman, J.; Omair, M.; Din, R.U.; Hassan, A.

2010-01-01

Mycobacterium fortuitum, a rapidly growing mycobacterium, is ubiquitous in nature. The organism was considered to be a harmless saprophyte but now there have been several reports from different parts of the world wherein it has been incriminated in a variety of human infections. We report a culture positive case of surgical site infection caused by Mycobacterium fortuitum, who responded well to the treatment. (author)

Terapia fotodinámica in vitro con Hipericina en hongos causantes de micosis cutáneas

OpenAIRE

Paz Cristóbal, Manuel Pablo de la; Pardo Jimeno, Julián; Gilaberte Calzada, Yolanda; Rezusta López, Antonio

2013-01-01

Las infecciones cutáneas o micosis son muy frecuentes, especialmente las causadas por bacterias y hongos. Dentro de las del segundo tipo, denominadas micosis, se encuentran las producidas por hongos dermatofitos o las causadas por levaduras, especialmente por Candida spp. (15). Un uso abusivo de las sustancias antibióticas clásicas, junto con la inapropiada prescripción médica promueven la generación de resistencias de estos microorganismos frente a los agentes antifúngicos. Además, en los úl...

Aspectos relevantes del uso de Mycobacterium´habana´ como candidato vacunal contra la tuberculosis

Directory of Open Access Journals (Sweden)

Iliana Valdés

2011-12-01

Full Text Available La eficacia protectora de la actual vacuna (BCG contra la tuberculosis, para contrarrestar las formas pulmonares de esta enfermedad y su reactivación, resulta variable o poco eficiente, lo cual impone la búsqueda urgente de nuevas alternativas profilácticas contra esta enfermedad. Basados en las ventajas inmunogénicas que ofrece el uso de vacunas vivas, se han encaminado diferentes estrategias de este tipo empleando mutantes auxotróficos de Mycobacterium tuberculosis , BCG recombinante o micobacterias no tuberculosas. Existen evidencias experimentales acerca de la protección conferida tras la vacunación con cepas vivas, inactivadas o fracciones proteicas de Mycobacterium'habana' TMC-5135 contra la infección por Mycobacterium tuberculosis. Esta respuesta protectora parece ir aparejada de escasos signos de virulencia en los modelos animales ensayados, lo cual coloca a M.´habana´ dentro de los posibles candidatos vacunales contra la tuberculosis al ajustarse a la condición que impone una vacuna clásica de reproducir la infección y los eventos inmunes que le suceden lo más fielmente posible a como ocurren de manera natural, sin causar extensos daños en el receptor.

Infecções intramamárias causadas por Staphylococcus aureus e suas implicações em paúde pública Staphylococcus aureus intramammary infections and its implications in public health

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Helena Fagundes

2004-08-01

Full Text Available Neste trabalho, são apresentados os principais problemas decorrentes das infecções intramamárias (mastites causadas por Staphylococcus aureus e as conseqüências para a saúde humana da veiculação de suas toxinas através do leite. o S. aureus destaca-se como um dos microorganismos mais importantes que podem ser transmitidos através dos alimentos. Assim, discute-se a possibilidade de veiculação de gastroenterite estafilocócica, não somente através do consumo de leite cru contaminado, mas também de leite tratado termicamente ou de derivados lácteos contendo enterotoxinas termoestáveis. São apresentados alguns aspectos relacionados ao potencial toxigênico das cepas de S. aureus, bem como as principais características das enterotoxinas estafilocócicas. Considerando que o S. aureus é um dos agentes de mastite mais freqüentemente observados, apresentam-se as principais medidas de controle de infecções estafilocócicas em rebanhos leiteiros, com vistas à prevenção da ocorrência de toxinas no leite de consumo.This article presents the main problems derived from the mammary infections (mastitis caused by Staphylococcus aureus, and the consequences of the presence of its toxins in milk for human health. S. aureus is one of the most important microorganisms that can be transmitted through the food products. Hence, the possibility of transmission of stafilococal gastroenteritis by consumption of raw milk and heat-treated milk, containing heat-resistant enterotoxins, is discussed. Some aspects regarding the toxigenic potential of S. aureus strains and the main characteristics of stafilococal entorotoxins are presented. Taking into account that S. aureus is also one of the most prevalent agents of mastitis, considerations are made on the methods for the controlling of stafilococal infections in dairy cattle, in order to prevent the occurrence of toxins in milk and milk products.

Detection of Mycobacterium bovis and Mycobacterium tuberculosis from Cattle: Possible Public Health Relevance

DEFF Research Database (Denmark)

Thakur, Aneesh; Sharma, Mandeep; Katoch, Vipin C.

2012-01-01

Mycobacterium bovis and Mycobacterium tuberculosis infect both animals and humans. The disease epidemiology by these agents differs in developed and developing countries due to the differences in the implementation of the prevention and control strategies. The present study describes the detectio...

Mycobacterium komaniense sp. nov., a rapidly growing non-tuberculous Mycobacterium species detected in South Africa.

Science.gov (United States)

Gcebe, Nomakorinte; Rutten, Victor P M G; van Pittius, Nicolaas Gey; Naicker, Brendon; Michel, Anita L

2018-05-01

Some species of non-tuberculous mycobacteria (NTM) have been reported to be opportunistic pathogens of animals and humans. Recently there has been an upsurge in the number of cases of NTM infections, such that some NTM species are now recognized as pathogens of humans and animals. From a veterinary point of view, the major significance of NTM is the cross-reactive immune response they elicit against Mycobacterium bovis antigens, leading to misdiagnosis of bovine tuberculosis. Four NTM isolates were detected from a bovine nasal swab, soil and water, during an NTM survey in South Africa. These were all found using 16S rRNA gene sequence analysis to be closely related to Mycobacterium moriokaense. The isolates were further characterised by sequence analysis of the partial fragments of hsp65, rpoB and sodA. The genome of the type strain was also elucidated. Gene (16S rRNA, hsp65, rpoB and sodA) and protein sequence data analysis of 6 kDa early secretory antigenic target (ESAT 6) and 10 kDa culture filtrate protein (CFP-10) revealed that these isolates belong to a unique Mycobacterium species. Differences in phenotypic and biochemical traits between the isolates and closely related species further supported that these isolates belong to novel Mycobacterium species. We proposed the name Mycobacterium komaniense sp. nov. for this new species. The type strain is GPK 1020 T (=CIP 110823T=ATCC BAA-2758).

Alterações anatômicas induzidas por Meloidogyne enterolobii (=M. mayaguensis e Meloidogyne javanica em tomateiros resistentes a meloidoginose

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Juliana Nogueira Westerich

2012-09-01

Full Text Available A resistência de tomateiros (Solanum lycopersicum L. a M. incognita, M. javanica e M. arenaria, conferida pela presença do gene Mi, não contempla a espécie M. enterolobii (=M. mayaguensis. O objetivo da pesquisa foi verificar as alterações anatômicas causadas por M. enterolobii no sistema radicular de porta-enxertos de tomateiro com o gene de resistência Mi ('Magnet' e Helper M' e compará-las com as causadas por M. javanica. As observações anatômicas das raízes foram feitas com auxílio de microscópio de luz e os aspectos mais relevantes foram fotografados. Com base em contagens e mensurações do tamanho dos sítios de alimentação e das células gigantes, foram efetuadas analises utilizando o método estatístico de Análise de Agrupamento. O aparecimento de células nutridoras incitadas por M. enterolobii foi verificado em ambos os porta-enxertos de tomateiro, entre 10 e 17 dias após a inoculação (DAI. O número e a área de sítios de alimentação e de células gigantes foram menores aos 17 DAI do que aos 24 DAI. Nesta época (24 DAI, foram observados sítios de alimentação constituídos pela presença de várias células nutridoras multinucleadas, com parede celular espessa, citoplasma denso e granuloso. Os tecidos vasculares apresentaram-se comprimidos e desorganizados, foi observada, também, hipertrofia de células do parênquima cortical. As raízes inoculadas com M. javanica não apresentaram alterações anatômicas.

Mortalidad por envenenamiento en niños

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Híjar Martha

1998-01-01

Full Text Available Objetivo. Conocer el panorama de las muertes por envenenamiento en niños de 0-14 años ocurridas en la República mexicana, entre 1979 y 1994. Material y métodos. Se utilizaron fuentes secundarias. Las variables analizadas fueron: edad, sexo, año, causa externa de traumatismos y envenenamientos, de la IX Clasificación Internacional de Enfermedades: E850-E858, E860-E869 y E905. Mediante un modelo de regresión Poisson se analizaron tendencias por causa específica y se obtuvieron riesgos relativos según edad, sexo y entidad federativa. Resultados. Hubo un total de 11 272 defunciones en menores de 15 años; las principales causas fueron el envenenamiento y las reacciones tóxicas causadas por plantas y animales venenosos (E905, el envenenamiento accidental por gas de uso doméstico y por monóxido de carbono (E868 y el envenenamiento accidental por otras drogas (E858. El grupo de edad que presentó los mayores riesgos, para las causas mencionadas, fue el de menores de un año con un riesgo relativo (RR de 29.6, IC95% 29.2-33.4; RR 3.47, IC95% 2.86-4.22, y RR 31.86, IC95% 24.8-40.9. El riesgo fue similar en ambos sexos, salvo para la causa E905. El estado de Aguascalientes se situó sistemáticamente entre los de mayor riesgo para todas las causas analizadas, mientras que Nuevo León siempre se ubicó entre los de riesgo más bajo. Conclusiones. El envenenamiento constituye una importante causa de muerte en los niños; el riesgo se incrementa al disminuir la edad. Considerando que esas muertes son potencialmente evitables y que la mayor parte de los envenenamientos ocurren en el hogar, para prevenirlos, se recomienda a los familiares vigilar y mantener fuera de peligro al niño. Por otra parte, la multicausalidad del fenómeno requiere que su prevención se realice desde una perspectiva multidisciplinaria que genere una cultura y un ambiente de seguridad en la sociedad.

Puccinia kuehnii (KRÜGER) BUTLER Y Puccinia melanocephala H. SYDOW Y P. SYDOW. EN EL CULTIVO DE LA CAÑA DE AZÚCAR

OpenAIRE

Infante, Danay; Martínez, B; González, E; González, Noyma

2009-01-01

La caña de azúcar es atacada por dos especies de Puccinia: la roya carmelita causada por P. melanocephala y la anaranjada por P. kuehnii. La primera ha prevalecido en el continente Americano, mientras que la segunda en Asia y Oceanía. Estas royas se diferencian respecto a las condiciones ambientales, la forma de presentarse el síntoma y el lugar de desarrollo del mismo. La mayor severidad causada por la roya carmelita se manifiesta durante los periodos fríos, en plantaciones de tres a seis me...

Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization

CSIR Research Space (South Africa)

Gcebe, N

2017-04-01

Full Text Available Journal of Systematic and Evolutionary Microbiology: DOI 10.1099/ijsem.0.001678 Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization Gcebe N Rutten V Gey...

Mycobacterium avium Infection after Acupoint Embedding Therapy

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Jiao Zhang, MD

2017-09-01

Full Text Available Summary:. Nontuberculous mycobacterium is a ubiquitous environmental organism that is unusual to cause a true infection, but it can cause severe cutaneous infections. In this case report, we present a successful treatment for a Chinese patient with Mycobacterium avium cutaneous infection after acupoint embedding therapy. We managed to conduct pathogenic detection, drug sensitive test, and multidisciplinary consultation. Finally, a systematic treatment strategy of nontuberculous mycobacterium was performed. Twenty-two-month follow-up revealed excellent outcome without any recurrence.

Caracterización de la resistencia a la antracnosis causada por Glomerella cingulata y productividad de cinco genotipos de mora (Rubus glaucus Benth.

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Juan Manuel López-Vásquez

2013-04-01

Full Text Available En la vereda Betania, municipio de Guática, Risaralda (Colombia a 2160 m.s.n.m., se evaluaron cinco materiales promisorios de mora (Rubus glaucus Benth. por su resistencia a la antracnosis (Colletotrichum gloeosporioides y por su alta productividad, los cuales fueron previamente seleccionados por el grupo de Investigación en Biodiversidad y Biotecnología de la Universidad Tecnológica de Pereira. Diez meses después del establecimiento las plantas fueron inoculadas con diferentes cepas del hongo a una concentración de 1.2 x 10(6 conidios/ml de agua. Las variables evaluadas fueron incidencia (presencia o ausencia de lesiones en tallo, severidad (según escala de 1 a 9 y rendimiento (kg/planta. Los genotipos se clasificaron en tres grupos según su resistencia al patógeno y por su rendimiento: el primero, lo conformaron UTP-8 y UTP-7, los cuales produjeron 21 y 17.6 t/ha, respectivamente; el segundo grupo estuvo formado por el genotipo UTP-1 el cual produjo 14.3 t/ha, y el tercero por UTP-2 y UTP-4 con 9.8 y 7.9 t/ha, respectivamente.

REPRESENTAÇÕES SOCIAIS SOBRE AS CAUSAS DOS ACIDENTES COM MATERIAIS PERFUROCORTANTES POR TÉCNICOS DE ENFERMAGEM

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Juliana Almeida Marques Lubenow

2012-01-01

Full Text Available Los accidentes con material corto-punzante son serios debido a las enfermedades que puedan ser causadas. El estudio es descriptivo, con abordaje cualitativa e fue desarrollado en hospital privado en Piauí, entre marzo y junio de 2011. El objetivo es analizar las causas atribuidas por técnicos de enfermería al su proprio accidente, utilizando la Teoría de las Representaciones Sociales. Fueran hechas entrevistas en profundidad con 16 profesionales que sufrirán ese tipo de accidente. Los dados fueran procesados a través del programa ALCESTE 4.8 que evidenció falta de cuidado como la principal causa identificada por elles. También fueran apuntadas negligencia del colega de trabajo e agitación del paciente. La representación social predominante es que el accidente solamente ocurre pelo comportamiento irresponsable del profesional. Por lo tanto, cada exposición percutánea debe ser analizada individualmente en la búsqueda de causas reales que non culpen solamente el trabajador.

Aspectos patológicos da intoxicação por aceturato de diminazeno em camelídeos sul-americanos

OpenAIRE

Seixas, Josilene N.; Orlando, Débora R.; Wouters, Flademir; Wouters, Angelica T.B.; Varaschin, Mary S.; Raymundo, Djeison L.

2017-01-01

RESUMO: Aceturato de diminazeno é um fármaco quimioterápico sintético comumente usado na medicina veterinária para o tratamento de doenças causadas por parasitos hematozoários. Entretanto, seu uso pode levar a efeitos colaterais, como alterações neurológicas graves e morte. A criação de camelídeos é uma atividade recente no Brasil, fazendo-se necessário conhecer mais sobre as doenças que acometem essas espécies. De dez camelídeos (seis lhamas e quatro alpacas) da propriedade, seis tiveram sin...

Aspectos anatomopatológicos em cães naturalmente infectados por Hepatozoon canis

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Pâmela A. Lima

Full Text Available RESUMO: A hepatozoonose canina é causada principalmente pelos protozoários Hepatozoon canis e H. americanum, transmitida por ingestão de carrapatos parasitados. Os sinais clínicos podem ser inespecíficos ou de difícil reconhecimento, pois geralmente ocorre associada a outras doenças. No Brasil, o parasito, e a doença, já foram identificados em vários Estados, no entanto pouco se sabe sobre as alterações clínicas e anátomo-patológicas decorrentes da infecção. O presente trabalho relata cinco casos de infecções naturais por Hepatozoon canis em cães do Estado de Minas Gerais e descreve pela primeira vez no Brasil os achados de necropsias e histopatológicos relacionados à infecção. Merontes de Hepatozoon sp., submetidos a avaliação morfométrica, foram observados em cortes histológicos de fígado, baço, medula óssea e rim.

Comparison of the UDP-N-Acetylmuramate:l-Alanine Ligase Enzymes from Mycobacterium tuberculosis and Mycobacterium leprae

OpenAIRE

Mahapatra, Sebabrata; Crick, Dean C.; Brennan, Patrick J.

2000-01-01

In the peptidoglycan of Mycobacterium leprae, l-alanine of the side chain is replaced by glycine. When expressed in Escherichia coli, MurC (UDP-N-acetyl-muramate:l-alanine ligase) of M. leprae showed Km and Vmax for l-alanine and glycine similar to those of Mycobacterium tuberculosis MurC, suggesting that another explanation should be sought for the presence of glycine.

Avaliação do estado de ativação e da produção de moleculas citotoxicas por linfocitos (CD4+ e CD8+) do sangue periferico de pacientes com paracoccidioidomicose

OpenAIRE

Lanny Cristina Burlandy Soares

2008-01-01

Resumo: Em doenças causadas por microorganismos intracelulares como a tuberculose, criptococose e listeriose foi demonstrado que as células T CD8+ contribuem de forma relevante para o controle da infecção. Em trabalho anterior verificamos um aumento do número de linfócitos T CD8+ no lavado broncoalveolar de pacientes com paracoccidioidomicose (PCM) pulmonar, sugerindo um papel para estas células, cuja ação efetora se dá por meio da produção de citocinas como o IFN-y e atividade citotóxica. O ...

Seca dos ponteiros da goiabeira causada por Erwinia psidii: níveis de incidência e aspectos epidemiológicos Guava bacterial blight due to Erwinia psidii: incidence levels and epidemiological aspects

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Abi Soares Anjos Marques

2007-01-01

Full Text Available Um dos fatores limitantes ao cultivo da goiabeira no Brasil é a 'seca dos ponteiros', causada por Erwinia psidii, presente nas regiões Sudeste e Centro-Oeste, onde se concentram grandes áreas produtoras. Considerando a pequena disponibilidade de informações sobre a epidemiologia e níveis de incidência dessa bacteriose, este estudo teve como objetivos: confirmar a distribuição e verificar a dispersão da seca dos ponteiros da goiabeira no Distrito Federal; investigar o efeito da temperatura sobre a multiplicação in vitro de E. psidii; desenvolver um teste de patogenicidade prático e eficiente e avaliar a sobrevivência in vitro da bactéria em diferentes substratos. A doença foi identificada em 56% das propriedades produtoras avaliadas no DF, com 81,9% de correlação entre a presença de sintomas e o diagnóstico laboratorial. A melhor faixa de temperatura para multiplicação de E. psidii foi de 24 a 33 ºC, e a bactéria permaneceu viável por até 120 dias em suspensão em água. A inoculação da bactéria em folhas ou hastes destacadas levou ao aparecimento de sintomas a partir do sétimo dia e mostrou-se eficiente como um teste rápido para se avaliar a patogenicidade de isolados.A major disease that affects guava is 'bacterial blight', caused by Erwinia psidii, which has been reported in Southeastern and Central Regions of Brazil where the major producing areas are located. Considering the lack of information on epidemiology and incidence levels of this disease, the objectives of this study were to confirm the presence and to verify the spread of the disease in Distrito Federal (DF; to determine optimal temperature for in vitro multiplication of E. psidii; to develop a simple and effective method for pathogenicity testing and to evaluate in vitro bacterial survival on different substrates. The disease was detected in 56% of producing orchards evaluated in DF, with a correlation of 81, 9% between presence of symptoms and

Whole genome sequence analysis of Mycobacterium suricattae

KAUST Repository

Dippenaar, Anzaan; Parsons, Sven David Charles; Sampson, Samantha Leigh; Van Der Merwe, Ruben Gerhard; Drewe, Julian Ashley; Abdallah, Abdallah; Siame, Kabengele Keith; Gey Van Pittius, Nicolaas Claudius; Van Helden, Paul David; Pain, Arnab; Warren, Robin Mark

2015-01-01

Tuberculosis occurs in various mammalian hosts and is caused by a range of different lineages of the Mycobacterium tuberculosis complex (MTBC). A recently described member, Mycobacterium suricattae, causes tuberculosis in meerkats (Suricata suricatta) in Southern Africa and preliminary genetic analysis showed this organism to be closely related to an MTBC pathogen of rock hyraxes (Procavia capensis), the dassie bacillus. Here we make use of whole genome sequencing to describe the evolution of the genome of M. suricattae, including known and novel regions of difference, SNPs and IS6110 insertion sites. We used genome-wide phylogenetic analysis to show that M. suricattae clusters with the chimpanzee bacillus, previously isolated from a chimpanzee (Pan troglodytes) in West Africa. We propose an evolutionary scenario for the Mycobacterium africanum lineage 6 complex, showing the evolutionary relationship of M. africanum and chimpanzee bacillus, and the closely related members M. suricattae, dassie bacillus and Mycobacterium mungi.

Whole genome sequence analysis of Mycobacterium suricattae

KAUST Repository

Dippenaar, Anzaan

2015-10-21

Tuberculosis occurs in various mammalian hosts and is caused by a range of different lineages of the Mycobacterium tuberculosis complex (MTBC). A recently described member, Mycobacterium suricattae, causes tuberculosis in meerkats (Suricata suricatta) in Southern Africa and preliminary genetic analysis showed this organism to be closely related to an MTBC pathogen of rock hyraxes (Procavia capensis), the dassie bacillus. Here we make use of whole genome sequencing to describe the evolution of the genome of M. suricattae, including known and novel regions of difference, SNPs and IS6110 insertion sites. We used genome-wide phylogenetic analysis to show that M. suricattae clusters with the chimpanzee bacillus, previously isolated from a chimpanzee (Pan troglodytes) in West Africa. We propose an evolutionary scenario for the Mycobacterium africanum lineage 6 complex, showing the evolutionary relationship of M. africanum and chimpanzee bacillus, and the closely related members M. suricattae, dassie bacillus and Mycobacterium mungi.

Comparison of the UDP-N-Acetylmuramate:l-Alanine Ligase Enzymes from Mycobacterium tuberculosis and Mycobacterium leprae

Science.gov (United States)

Mahapatra, Sebabrata; Crick, Dean C.; Brennan, Patrick J.

2000-01-01

In the peptidoglycan of Mycobacterium leprae, l-alanine of the side chain is replaced by glycine. When expressed in Escherichia coli, MurC (UDP-N-acetyl-muramate:l-alanine ligase) of M. leprae showed Km and Vmax for l-alanine and glycine similar to those of Mycobacterium tuberculosis MurC, suggesting that another explanation should be sought for the presence of glycine. PMID:11073931

Compromiso pulmonar en pacientes hospitalizados con infección por el virus de inmunodeficiencia humana en el Hospital Pablo Tobón Uribe (Medellín, Colombia

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Franco E. Montúfar-Andrade

Full Text Available Resumen Más de la mitad de los pacientes con infección por virus de inmunodeficiencia humana (VIH pueden tener compromiso pulmonar en el transcurso de su vida. Este puede ser multicausal y las infecciones oportunistas son las principales, sin embargo, las causas no infecciosas no son menos importantes. Objetivo: Describir las características del compromiso pulmonar en pacientes hospitalizados con infección por VIH. Metodología: Estudio descriptivo observacional. Resultados: Se incluyó a 63 pacientes, el 85,7% fueron hombres. La edad promedio fue 40,6 ± 12 años. El 21,4% tuvieron antecedente de promiscuidad sexual y 28,6% fueron hombres con sexo con hombres. Se identificó tabaquismo en 60,3%, alcoholismo en 52,4% y consumo de drogas en 20,6%. El 23,8% tuvo historia de tuberculosis. Al ingreso, el 79,4% tenía sida, el recuento promedio de CD4 fue 138,5 ± 17,7 células/mm3 y el 49,2% tenía CD4 < 100 células/mm3. Las principales comorbilidades fueron: enfermedad neoplásica 20,6%, diarrea crónica 19%, EPOC 6,3% y diabetes mellitus 3,2%. Los principales síntomas al ingreso fueron: respiratorios 66,7%, gastrointestinales 47,6% y neurológicos 34,9%. Los principales oportunistas documentados fueron Mycobacterium spp, H. capsulatum y P. jirovecii. El sarcoma de Kaposi, la enfermedad lifoproliferativa y la EPOC fueron causas de compromiso no infeccioso. Los principales hallazgos radiográficos fueron: infiltrados intersticiales (42,9%, compromiso pleural (23,8% y lesiones cavitarias (7,9%. El compromiso multilobar fue evidente en el 15,9%. Las principales complicaciones fueron falla respiratoria, disfunción orgánica múltiple y falla renal aguda. El 19% requirió ingreso a UCI y, de estos, el 83,3% necesitó ventilación mecánica. La estancia hospitalariapromedio fue de 18 ± 20 días y la mortalidad del 17,5%. Discusión: Las infecciones por oportunistas son las principales causas de compromiso pulmonar y, dentro de estas, las causadas

Drug Resistance of Mycobacterium tuberculosis Complex among ...

African Journals Online (AJOL)

BACKGROUND: In Burkina Faso, there is no recent data about the level of drug resistance in Mycobacterium tuberculosis strains among newly diagnosed tuberculosis cases. OBJECTIVE: To provide an update of the primary drug resistance of mycobacterium tuberculosis among patients in Burkina faso. METHODS: ...

Molecular Characterization of the Resistance of Mycobacterium ...

African Journals Online (AJOL)

Purpose: To characterize the resistance of Mycobacterium tuberculosis to second line drugs using a line probe assay. Methods: Multi-drug resistant strains of Mycobacterium tuberculosis isolated between December 2008 and December 2009 were tested for resistance to fluoroquinolones and second-line injectable drugs ...

Tuberculosis Multidrogoresistente

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German A Acevedo

2013-12-01

Full Text Available La tuberculosis es una enfermedad infecciosa causada por el Mycobacterium tuberculosis. En el año 2010 se registraron 8.8 millones de casos incidentes en el mundo y en los últimos años han aparecido poblaciones bacterianas de micobacterias con resistencia a los fármacos de primera línea. Se ha definido la presencia de resistencia a rifampicina e isoniacida como multidrogoresistencia, estimándose una incidencia mundial aproximada de 3.6%. Esta revisión de tema se centrará en la situación de la tuberculosis multidrogoresistente en el mundo, incluyendo un análisis regional de la casuística Colombiana. Se comentarán los principales mecanismos de resistencia del microorganismo, los genes implicados en la misma y los factores de riesgo asociados a la generación de resistencia en algunas comunidades.

Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.

Science.gov (United States)

Gupta, Radhey S; Lo, Brian; Son, Jeen

2018-01-01

The genus Mycobacterium contains 188 species including several major human pathogens as well as numerous other environmental species. We report here comprehensive phylogenomics and comparative genomic analyses on 150 genomes of Mycobacterium species to understand their interrelationships. Phylogenetic trees were constructed for the 150 species based on 1941 core proteins for the genus Mycobacterium , 136 core proteins for the phylum Actinobacteria and 8 other conserved proteins. Additionally, the overall genome similarity amongst the Mycobacterium species was determined based on average amino acid identity of the conserved protein families. The results from these analyses consistently support the existence of five distinct monophyletic groups within the genus Mycobacterium at the highest level, which are designated as the " Tuberculosis-Simiae ," " Terrae," " Triviale ," " Fortuitum-Vaccae ," and " Abscessus-Chelonae " clades. Some of these clades have also been observed in earlier phylogenetic studies. Of these clades, the " Abscessus-Chelonae" clade forms the deepest branching lineage and does not form a monophyletic grouping with the " Fortuitum-Vaccae " clade of fast-growing species. In parallel, our comparative analyses of proteins from mycobacterial genomes have identified 172 molecular signatures in the form of conserved signature indels and conserved signature proteins, which are uniquely shared by either all Mycobacterium species or by members of the five identified clades. The identified molecular signatures (or synapomorphies) provide strong independent evidence for the monophyly of the genus Mycobacterium and the five described clades and they provide reliable means for the demarcation of these clades and for their diagnostics. Based on the results of our comprehensive phylogenomic analyses and numerous identified molecular signatures, which consistently and strongly support the division of known mycobacterial species into the five described clades, we

Phylogenetic analysis of vitamin B12-related metabolism in Mycobacterium tuberculosis

OpenAIRE

Young, Douglas B.; Comas, I?aki; de Carvalho, Luiz P. S.

2015-01-01

Comparison of genome sequences from clinical isolates of Mycobacterium tuberculosis with phylogenetically-related pathogens Mycobacterium marinum, Mycobacterium kansasii, and Mycobacterium leprae reveals diversity amongst genes associated with vitamin B12-related metabolism. Diversity is generated by gene deletion events, differential acquisition of genes by horizontal transfer, and single nucleotide polymorphisms (SNPs) with predicted impact on protein function and transcriptional regulation...

Cura espontânea da leishmaniose causada por Leishmania Viannia Braziliensis em lesões cutâneas

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Jackson Maurício Lopes Costa

1990-12-01

Full Text Available Os autores relatam que durante 14 anos de trabalho clínico em campo, realizado nas comunidades de Três Braços e Corte de Pedra, Bahia, acompanharam 1.416 pacientes portadores de Leishmaniose Tegumentar Americana, cuja espécie envolvida na transmissão, é predominantemente a Leishmania Viannia brasilienses. A terapêutica utilizada rotineiramente nos casos é o antimoniato-N-metilglucamina (Glucantime. Contudo, 16 pacientes do sexo masculino recusaram-se a utilizar a medicação e 6 do sexo feminino encontravam-se em período gestacional, portanto não utilizaram o medicamento. Estes pacientes foram acompanhados por um período entre 4 a 12 anos, a partir do diagnóstico. Observou-se que em 9 pacientes (40,9% desta casuística, o tempo de cicatrizaçâo após o aparecimento da lesão, pode ser calculado em 6 meses de evolução. Quando se eleva a observação para 12 meses, temos que 19 pacientes (86,3% cicatrizaram suas lesões neste período. Em 3 casos (13,6% as lesões permaneceram ativas por mais de 12 meses. Conclui-se que os determinantes da cicatrizaçâo natural das lesões produzidas por Leishmania Viannia Braziliensis permanecem desconhecidos, dificultando para nós entendermos e compararmos aos efeitos das drogas utilizadas no tratamento da leishmaniose tegumentar.In field clinics in the comunities of Três Braços and Corte de Pedra, Bahia, we have attended 1.416 patients with tegumentary leishmaniasis in fourteen years, the predomi nant species in transmission is Leishmania Viannia brasiliensis (LVB. Because of the danger of metastasis with this infection treatment was routinely recomended with Glucantime. However sixteen patients refused injection therapy and six women were pregnant when seen and not treated. All patients were followed up in our clinic. All these patients closed their skin ulcers although one subsequently relapsed. Patients were followed up for variable periods (four to twelve years, after the diagnosis. In

COMPORTAMIENTO ESPACIAL DE TRIPS (INSECTA: THYSANOPTERA), EN PLANTACIONES COMERCIALES DE AGUACATE (PERSEA AMERICANA MILL.) EN ZITÁCUARO, MICHOACÁN, MÉXICO

OpenAIRE

José Francisco RAMÍREZ DÁVILA

2013-01-01

México es el principal productor y exportador de aguacate en el mundo. Los trips son considerados como una de las principales plagas del aguacate en México, debido a su asociación con la enfermedad denominada roña del fruto, causada por el hongo Elsinoe perseae. Esta enfermedad representa una fuerte limitante a la producción del cultivo; el hongo penetra la fruta por las heridas causadas por el complejo de varias especies de trips. El análisis del comportamiento espacial de esta plaga proporc...

Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., isolated from the pitcher plant Sarracenia purpurea.

Science.gov (United States)

Tran, Phuong M; Dahl, John L

2016-11-01

Several fast- to intermediate-growing, acid-fast, scotochromogenic bacteria were isolated from Sarracenia purpurea pitcher waters in Minnesota sphagnum peat bogs. Two strains (DL734T and DL739T) were among these isolates. On the basis of 16S rRNA gene sequences, the phylogenetic positions of both strains is in the genus Mycobacterium with no obvious relation to any characterized type strains of mycobacteria. Phenotypic characterization revealed that neither strain was similar to the type strains of known species of the genus Mycobacterium in the collective properties of growth, pigmentation or fatty acid composition. Strain DL734T grew at temperatures between 28 and 32 °C, was positive for 3-day arylsulfatase production, and was negative for Tween 80 hydrolysis, urease and nitrate reduction. Strain DL739T grew at temperatures between 28 and 37 °C, and was positive for Tween 80 hydrolysis, urea, nitrate reduction and 3-day arylsulfatase production. Both strains were catalase-negative while only DL739T grew with 5 % NaCl. Fatty acid methyl ester profiles were unique for each strain. DL739T showed an ability to survive at 8 °C with little to no cellular replication and is thus considered to be psychrotolerant. Therefore, strains DL734T and DL739T represent two novel species of the genus Mycobacterium with the proposed names Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., respectively. The type strains are DL734T (=JCM 30395T=NCCB 100519T) and DL739T (=JCM 30396T=NCCB 100520T), respectively.

A single or multistage mycobacterium avium subsp. paratuberculosis subunit vaccine

DEFF Research Database (Denmark)

2014-01-01

The present invention provides one or more immunogenic polypeptides for use in a preventive or therapeutic vaccine against latent or active infection in a human or animal caused by a Mycobacterium species, e.g. Mycobacterium avium subsp. paratuberculosis. Furthermore a single or multi-phase vaccine...... comprising the one or more immunogenic polypeptides is provided for administration for the prevention or treatment of infection with a Mycobacterium species, e.g. Mycobacterium avium subsp. paratuberculosis. Additionally, nucleic acid vaccines, capable of in vivo expression of the multi-phase vaccine...

La elasticidad de la demanda por electricidad y la política energética

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Alexander Galetovic

2010-01-01

Full Text Available En el análisis y la práctica de la política energética se suele suponer que la elasticidad de la demanda por energía eléctrica es irrelevante. Este trabajo muestra que durante episodios de escasez de energía una “pequeña” elasticidad basta para generar caídas “grandes” de la probabilidad de déficit y del costo del abastecimiento eléctrico. Esto se debe a que en la vecindad de la capacidad del sistema, la oferta de energía de corto plazo es cercana a vertical. Ilustramos nuestro punto cuantitativamente simulando la operación esperada del sistema eléctrico chileno durante los años de ajuste a la crisis causada por los cortes de gas argentinos, el periodo 2006-2010. Mostramos que el aumento de los precios causado por el retraso de las inversiones y los cortes de gas argentino, combinado con una “pequeña” elasticidad de la demanda mensual por energía (0.0548 en valor absoluto eran suficientes para reducir mucho la probabilidad de déficit mensual y retornarla a niveles normales. Más aún, si se soslaya el efecto de los mayores precios en el consumo, el costo marginal se sobreestima en 32% y el costo de operación esperado en 41 por ciento.

Encefalitis del tallo cerebral y mielitis por Listeria monocytogenes

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Aracelly Castro

2013-09-01

Full Text Available La romboencefalitis por Listeria monocytogenes es una presentación poco común de la listeriosis del sistema nervioso central; sin embargo, es la presentación más común en personas inmunocompetentes. Aun más rara es la combinación de romboencefalitis con mielitis causada por L. monocytogenes; no obstante, en este artículo se reporta un caso de encefalitis del tallo y mielitis grave en un paciente sin compromiso del sistema inmunitario. Se presenta un paciente de 21 años de edad, sin deficiencias del sistema inmunitario, que consumió productos lácteos no pasteurizados y, posteriormente, presentó un cuadro de cefalea, vómito, deterioro de su estado general y, finalmente, alteración del estado de conciencia y muerte. Consultó al Instituto Neurológico de Colombia y se hizo diagnóstico de encefalitis del tallo y mielitis por L. monocytogenes. Se discuten las diferencias entre el caso presentado y los reportados en la literatura científica. Ante un paciente con signos de compromiso del tallo cerebral, de posible origen infeccioso, es prudente iniciar tratamiento antibiótico para L. monocytogenes y, en caso de poca respuesta, escalar rápidamente en dicho tratamiento. También lo es extender el estudio radiológico hacia la columna vertebral, con el fin de descartar compromiso de la médula espinal.   doi: http://dx.doi.org/10.7705/biomedica.v33i3.1482

Disseminated Mycobacterium avium infection in a cat

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Barry, Maureen; Taylor, Judith; Woods, Paul

2002-01-01

A domestic shorthair cat was presented for lethargy and ataxia. Clinical findings included an abdominal mass, lumbosacral pain, ataxia. Aspirates from the liver and lymph nodes revealed intracellular, negative-staining rods. Treatment for presumptive mycobacterium infection was unsuccessful and the cat was euthanized. Disseminated Mycobacterium avium was confirmed on culture.

Disseminated Mycobacterium avium infection in a cat.

Science.gov (United States)

Barry, Maureen; Taylor, Judith; Woods, J Paul

2002-05-01

A domestic shorthair cat was presented for lethargy and ataxia. Clinical findings included an abdominal mass, lumbosacral pain, ataxia. Aspirates from the liver and lymph nodes revealed intracellular, negative-staining rods. Treatment for presumptive mycobacterium infection was unsuccessful and the cat was euthanized. Disseminated Mycobacterium avium was confirmed on culture.

Surto de mastite bovina causada por Arcanobacterium pyogenes

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R.G. Motta

2011-06-01

Full Text Available An uncommon outbreak of mastitis caused by Arcanobacterium pyogenes in 26 cows is reported. The epidemiological findings, clinical signs, microbiological exams, somatic cell count, in vitro susceptibility profile of strains, efficacy of intramammary treatment and control measures were discussed. Florfenicol (96.2%, cefoperazona (92.3%, cefaloxin (84.6% and ceftiofur (84.6% were the most effective antimicrobials, and neomicin (27.0% and enrofloxacin (17.4% the least effective antimicrobials.

Resíduos orgânicos para o controle das doenças do feijoeiro causadas por Sclerotium rolfsii / Organic residues for the control of bean diseases caused by Sclerotium rolfsii

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Vanessa Nataline Tomazeli

2011-04-01

Full Text Available A incorporação de matéria orgânica ao solo tem se mostrado eficiente para o controle de alguns fitopatógenos habitantes do solo. O efeito dessa prática agrícola, principalmente sobre patógenos formadores de escleródios, como Sclerotium rolfsii, ainda não é completamente conhecido. O experimento, em dois anos de cultivo, foi conduzido no campo experimental da Universidade Tecnológica Federal do Paraná (UTFPR, em parcelas de um metro quadrado, contendo solo infestado com 100 g por parcela do substrato (arroz em casca contendo o patógeno. Os tratamentos foram chorume de suínos, cama de aviário, repolho triturado e testemunha, apenas patógeno, com quatro repetições. A semeadura de 80 sementes de feijão por parcela foi efetuada após uma semana da aplicação dos respectivos tratamentos. A intensidade da doença foi avaliada pela emergência, tombamento, incidência e severidade da doença. Os fatores como atividade microbiana, níveis de fertilidade do solo, número e sobrevivência de escleródios e massa das plantas fresca foram avaliados no fim do cultivo. O composto orgânico, cama de aviário, apresentou uma maior redução da incidência e a severidade da doença e, consequentemente, o tombamento de plântulas. Porém, apesar de reduzir a intensidade da doença, não houve aumento da emergência nos dois primeiros cultivos. Esse efeito benéfico da cama-de-aviário (CA pode estar associado ao aumento da matéria orgânica do solo e, consequentemente, da atividade microbiana, principal responsável pela redução da patogenicidade do fungo. Como consequência ao impacto provocado pela incorporação de CA ao solo, houve aumento da massa fresca da planta e redução do número de escleródios. Assim, com base nesses resultados, pode-se concluir que a CA apresenta efeito supressivo sobre as doenças causadas por S. rolfsii.AbstractOrganic matter incorporation into the soil has proved effective for the control of some soil

Clonación, sobreexpresión y determinación de la especificidad iónica de Pma1, una posible Na+/K+ ATPasa de Mycobacterium smegmatis

OpenAIRE

Ayala Torres, Carlos Mario

2014-01-01

La tuberculosis (TB) es uno de los retos más importantes para la salud pública en el mundo. Actualmente existe una prevalencia de 2 billones de personas infectadas con Mycobacterium tuberculosis, lo que representa el 30% de la población mundial; en este contexto la TB junto con el VIH/SIDA (Virus de Inmunodeficiencia Humana/Síndrome de Inmunodeficiencia Adquirida) son las enfermedades que causan el mayor número de muertes ocasionadas por agentes infecciosos en el mundo, por lo ...

Comparative Genomics and Proteomic Analysis of Four Non-tuberculous Mycobacterium Species and Mycobacterium tuberculosis Complex : Occurrence of Shared Immunogenic Proteins

NARCIS (Netherlands)

Gcebe, Nomakorinte; Michel, Anita; Gey van Pittius, Nicolaas C; Rutten, Victor

2016-01-01

The Esx and PE/PPE families of proteins are among the most immunodominant mycobacterial antigens and have thus been the focus of research to develop vaccines and immunological tests for diagnosis of bovine and human tuberculosis, mainly caused by Mycobacterium bovis and Mycobacterium tuberculosis,

Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

Science.gov (United States)

Gupta, Radhey S.; Lo, Brian; Son, Jeen

2018-01-01

The genus Mycobacterium contains 188 species including several major human pathogens as well as numerous other environmental species. We report here comprehensive phylogenomics and comparative genomic analyses on 150 genomes of Mycobacterium species to understand their interrelationships. Phylogenetic trees were constructed for the 150 species based on 1941 core proteins for the genus Mycobacterium, 136 core proteins for the phylum Actinobacteria and 8 other conserved proteins. Additionally, the overall genome similarity amongst the Mycobacterium species was determined based on average amino acid identity of the conserved protein families. The results from these analyses consistently support the existence of five distinct monophyletic groups within the genus Mycobacterium at the highest level, which are designated as the “Tuberculosis-Simiae,” “Terrae,” “Triviale,” “Fortuitum-Vaccae,” and “Abscessus-Chelonae” clades. Some of these clades have also been observed in earlier phylogenetic studies. Of these clades, the “Abscessus-Chelonae” clade forms the deepest branching lineage and does not form a monophyletic grouping with the “Fortuitum-Vaccae” clade of fast-growing species. In parallel, our comparative analyses of proteins from mycobacterial genomes have identified 172 molecular signatures in the form of conserved signature indels and conserved signature proteins, which are uniquely shared by either all Mycobacterium species or by members of the five identified clades. The identified molecular signatures (or synapomorphies) provide strong independent evidence for the monophyly of the genus Mycobacterium and the five described clades and they provide reliable means for the demarcation of these clades and for their diagnostics. Based on the results of our comprehensive phylogenomic analyses and numerous identified molecular signatures, which consistently and strongly support the division of known mycobacterial species into the five

Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

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Radhey S. Gupta

2018-02-01

Full Text Available The genus Mycobacterium contains 188 species including several major human pathogens as well as numerous other environmental species. We report here comprehensive phylogenomics and comparative genomic analyses on 150 genomes of Mycobacterium species to understand their interrelationships. Phylogenetic trees were constructed for the 150 species based on 1941 core proteins for the genus Mycobacterium, 136 core proteins for the phylum Actinobacteria and 8 other conserved proteins. Additionally, the overall genome similarity amongst the Mycobacterium species was determined based on average amino acid identity of the conserved protein families. The results from these analyses consistently support the existence of five distinct monophyletic groups within the genus Mycobacterium at the highest level, which are designated as the “Tuberculosis-Simiae,” “Terrae,” “Triviale,” “Fortuitum-Vaccae,” and “Abscessus-Chelonae” clades. Some of these clades have also been observed in earlier phylogenetic studies. Of these clades, the “Abscessus-Chelonae” clade forms the deepest branching lineage and does not form a monophyletic grouping with the “Fortuitum-Vaccae” clade of fast-growing species. In parallel, our comparative analyses of proteins from mycobacterial genomes have identified 172 molecular signatures in the form of conserved signature indels and conserved signature proteins, which are uniquely shared by either all Mycobacterium species or by members of the five identified clades. The identified molecular signatures (or synapomorphies provide strong independent evidence for the monophyly of the genus Mycobacterium and the five described clades and they provide reliable means for the demarcation of these clades and for their diagnostics. Based on the results of our comprehensive phylogenomic analyses and numerous identified molecular signatures, which consistently and strongly support the division of known mycobacterial species

Mycobacterium chelonae infections associated with bee venom acupuncture.

Science.gov (United States)

Cho, Sun Young; Peck, Kyong Ran; Kim, Jungok; Ha, Young Eun; Kang, Cheol-In; Chung, Doo Ryeon; Lee, Nam Yong; Song, Jae-Hoon

2014-03-01

We report 3 cases of Mycobacterium chelonae infections after bee venom acupuncture. All were treated with antibiotics and surgery. Mycobacterium chelonae infections should be included in the differential diagnosis of chronic skin and soft tissue infections following bee venom acupuncture.

Draft Genome Sequence of Mycobacterium chimaera Type ...

Science.gov (United States)

We report the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169T, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, though Fl-0169T possesses unique virulence genes. Evidence suggests that M. avium, M. intracellulare, and M. chimaera are differently virulent and a comparative genomic analysis is critically needed to identify diagnostic targets that reliably differentiate species of MAC. With treatment costs for Mycobacterium infections estimated to be >$1.8 B annually in the U.S., correct species identification will result in improved treatment selection, lower costs, and improved patient outcomes.

Complete Genome Sequence of the Frog Pathogen Mycobacterium ulcerans Ecovar Liflandii

NARCIS (Netherlands)

Tobias, Nicholas J.; Doig, Kenneth D.; Medema, Marnix H.; Chen, Honglei; Haring, Volker; Moore, Robert; Seemann, Torsten; Stinear, Timothy P.

In 2004, a previously undiscovered mycobacterium resembling Mycobacterium ulcerans (the agent of Buruli ulcer) was reported in an outbreak of a lethal mycobacteriosis in a laboratory colony of the African clawed frog Xenopus tropicalis. This mycobacterium makes mycolactone and is one of several

Formulacio?n de mezclas de aceites esenciales como inhibidores de Listeria monocytogenes

OpenAIRE

Cazar R., Mari?a-Elena

2013-01-01

Las enfermedades causadas por el consumo de alimentos contaminados tienen un alto impacto econo?mico y en la salud pu?blica. Las contaminaciones de alimentos causadas por pato?genos como Campylobacter y Salmonella son las ma?s comu?nmente reportadas. No obstante, pato?genos como Listeria monocytogenes pueden adaptarse, sobrevivir y crecer en un amplio rango de condiciones ambientales, causando patologi?as con elevadas tasas de hospitalizacio?n y mortalidad (Gandhi y Chikindas,2007). Las in...

Cambiando los paradigmas de la infección por M. pneumoniae en pediatría

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Jocelin Merida-Vieyra

2017-11-01

Full Text Available La neumonía atípica es un término que originalmente se utilizó en pacientes adultos que presentaban neumonías de curso atípico, generalmente leves y de evolución benigna, que eran causadas por agentes como: Mycoplasma pneumoniae, Chlamydophila pneumoniae y Legionella pneumophila. En población pediátrica la presentación, tanto clínica como epidemiológica, de las neumonías por M. pneumoniae merece una especial atención ya que no siempre son cursos benignos y pueden existir numerosos cuadros extrapulmonares asociados; el proceso diagnóstico es complejo y poco diferencial de otros agentes etiológicos. Recientemente ha llamado la atención la emergencia de la resistencia a macrólidos en algunos países del mundo, por lo que existen controversias respecto al tratamiento. Estudios numerosos realizados a nivel mundial, particularmente derivados de países asiáticos, han generado nueva información en relación al comportamiento biológico, clínico, epidemiológico, radiológico y respuesta terapéutica de M. pneumoniae en pacientes pediátricos.

Mycobacterium angelicum sp. nov., a non-chromogenic, slow-growing species isolated from fish and related to Mycobacterium szulgai.

Science.gov (United States)

Pourahmad, Fazel; Pate, Mateja; Ocepek, Matjaž; Borroni, Emanuele; Cabibbe, Andrea M; Capitolo, Eleonora; Cittaro, Davide; Frizzera, Eliana; Jenčič, Vlasta; Mariottini, Alessandro; Marumo, Kenji; Vaggelli, Guendalina; Cirillo, Daniela M; Tortoli, Enrico

2015-12-01

The name 'Mycobacterium angelicum' dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. This name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. The four strains presented 100 % 16S rRNA gene sequence similarity with Mycobacterium szulgai but clearly differed from M. szulgai for the milky white aspect of the colonies. The sequence similarity with the type strain of M. szulgai ranged, in eight additionally investigated genetic targets, from 78.9 to 94.3 %, an evident contrast with the close relatedness that emerged at the level of 16S rRNA gene. The average nucleotide identity between the genomes of M. szulgai DSM 44166T and strain 126/5/03T (type strain of the novel species) was 92.92 %, and supported the status of independent species. The confirmation of the name Mycobacterium angelicum sp. nov. is proposed, with strain 126/5/03T ( = CIP 109313T = DSM 45057T) as the type strain.

PREVALÊNCIA DA Mycobacterium tuberculosis NO COMPLEXO PRISIONAL DO MUNICÍPIO DE ITAPERUNA, RJ

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Francislene FERREIRA SILVA

2015-12-01

Full Text Available A presença da tuberculose em sistemas prisionais é descrito como uma ameaça a saúde pública. O presente estudo objetivou a determinar a prevalência da Mycobacterium tuberculosis no sistema prisional Diomedes Vinhosa Muniz em Itaperuna, Rio de Janeiro. O estudo foi submetido e aprovado pelo comitê de ética em pesquisa da Faculdade Redentor em Itaperuna e autorizado pela Secretaria de Estado de Administração Penitenciária (SEAP do estado do Rio de Janeiro. Participaram da pesquisa 10% do total de detentos condenados, estes assinaram um termo de consentimento livre e esclarecido, preencheram um questionário sócio econômico e epidemiológico em seguida foi coletado o material biológico para realização da baciloscopia. Os resultados indicaram que 69% dos detentos analisados não completaram o ensino fundamental, 30% é originário de comunidades faveladas, 55% possuem uma renda mensal de até 2 salários mínimos, 79% consumiam tabaco, 64% relataram que tiveram tosse por mais de 3 semanas, 65% declaram a maconha como a droga ilícita preferencial e 51% usavam cocaína, 3% declararam se portador do HIV, 42% tiveram contato com indivíduos com a tuberculose e 3% dos detentos haviam a presença da Mycobacterium tuberculosis. Logo, conclui-se que no sistema prisional Diomedes Vinhosa Muniz houve uma baixa prevalência da M.ycobacterium tuberculosis.

Intoxicação por Enterolobium contortisiliquum em bovinos na região Nordeste do Brasil

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Roberio G. Olinda

2015-01-01

Full Text Available Descreve-se um surto de fotossensibilização causada por favas de Enterolobium contortisiliquum em bovinos no Estado de Pernambuco, Nordeste do Brasil. Os sinais clínicos observados foram hiporexia, prostração, perda de peso, edema generalizado, dermatite e icterícia. Havia anemia e atividades séricas de AST, LDH e GGT elevadas. A concentração sérica de ureia encontrava-se levemente aumentada e creatinina, proteínas séricas e albumina estavam em níveis normais. Na necropsia, o fígado estava aumentado de tamanho e difusamente alaranjado e os rins estavam aumentados de volume e apresentavam estriações esbranquiçadas irregulares entremeadas por áreas escuras na superfície subcapsular. Histologicamente, observou-se na pele, dermatite ulcerativa. No fígado havia vacuolização e necrose de hepatócitos da região centrolobular e ocasionalmente necrose individual de hepatócitos em outras regiões. O rim apresentava nefrose tubular tóxica. O diagnóstico diferencial da intoxicação por E. contortisiliquum na região semiárida deve incluir as intoxicações por Brachiaria spp, Panicum dichotomiflorum e Froelichia humboldtiana, que também causam fotossensibilização.

Atenção ao Suicídio

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Berenice Rheinreimer

2015-11-01

Full Text Available De acordo com a OMS a cada ano ocorrem 1 milhão de mortes causadas por suicídio, número maior que a soma das mortes causadas por guerras e homicídios (1. Até 2030, as mortes por suicídio podem chegar a 1 milhão e quinhentos mil, caso sejam mantidos os índices de crescimento atuais (2, 3. Para cada morte, ocorrem de 10 a 40 tentativas de suicídio, ou seja, 10 a 40 milhões de tentativas de suicídio ao ano (4.

Role of genotype® mycobacterium common mycobacteria/additional species assay for rapid differentiation between Mycobacterium tuberculosis complex and different species of non-tuberculous mycobacteria

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Amresh Kumar Singh

2013-01-01

Full Text Available Background: Mycobacterium tuberculosis complex (MTBC and non-tuberculous mycobacteria (NTM may or may not have same clinical presentations, but the treatment regimens are always different. Laboratory differentiation between MTBC and NTM by routine methods are time consuming and cumbersome to perform. We have evaluated the role of GenoType® Mycobacterium common mycobacteria/additional species (CM/AS assay for differentiation between MTBC and different species of NTM in clinical isolates from tuberculosis (TB cases. Materials and Methods: A total of 1080 clinical specimens were collected from January 2010 to June 2012. Diagnosis was performed by Ziehl-Neelsen staining followed by culture in BacT/ALERT 3D system (bioMerieux, France. A total of 219 culture positive clinical isolates (BacT/ALERT® MP cultures were selected for differentiation by p-nitrobenzoic acid (PNB sensitivity test as and BIO-LINE SD Ag MPT64 TB test considering as the gold standard test. Final identification and differentiation between MTBC and different species of NTM were further confirmed by GenoType® Mycobacterium CM/AS assay (Hain Lifescience, Nehren, Germany. Results: Out of 219 BacT/ALERT® MP culture positive isolates tested by PNB as 153 MTBC (69.9% and by GenoType® Mycobacterium CM/AS assay as 159 (72.6% MTBC and remaining 60 (27.4% were considered as NTM species. The GenoType® Mycobacterium CM/AS assay was proved 99.3% sensitive and 98.3% specific for rapid differentiation of MTBC and NTM. The most common NTM species were; Mycobacterium fortuitum 20 (33.3% among rapid growing mycobacteria and Mycobacterium intracellulare 11 (18.3% among slow growing mycobacteria. Conclusion: The GenoType® Mycobacterium assay makes rapid and accurate identification of NTM species as compared with different phenotypic and molecular diagnostic tool and helps in management of infections caused by different mycobacteria.

Determinación y prevalencia de Mycobacterium spp., en tilapia nilótica (Oreochromis niloticus cultivada en Campeche, México

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Maurilio Lara-Flores

2013-03-01

Full Text Available Objetivo. Determinar la presencia y prevalencia de Mycobacterium spp., en granjas de tilapia nilótica (Oreochromis niloticus en el Municipio de Champotón, Campeche, México. Materiales y métodos. La colecta de organismos se realizó en tres granjas de cultivo de tilapia nilótica del municipio de Champotón, Campeche, México. Los organismos se examinaron externa e internamente y se tomó una muestra de riñón la cual fue sembrada en forma de estría en medios de cultivo: Löwesntein-Jensen, TCBS, KF y en TSA; las placas fueron incubadas a 35°C de 24 a 48 horas, los órganos fueron fijados en formalina tamponada al 10% para ser procesados para histología de rutina para análisis posteriores. Asimismo, muestras de cultivo bacteriológico y de tejido fueron teñidas con la técnica de Ziel-Neelsen con el fin de observar la presencia de bacilos ácido-alcohol resistentes. Resultados. Los resultados obtenidos sugieren que la presencia de Mycobacterium spp., es constante y en alta prevalencia y puede ser un factor que este mermando la rentabilidad del cultivo. Conclusiones. La presencia de Mycobacterium spp., representa un riesgo para el cultivo de tilapia en el municipio de Champotón, por ser una enfermedad muy persistente y difícil de erradicar una vez ocurrido e brote de infección, por lo cual es importante llevar estudios más detallados de la presencia de este género bacteriano, así como, medidas de prevención y dispersión de este patógeno en los cultivos adyacentes.

Cytochemical and biological properties of Mycobacterium bovis BCG.

Science.gov (United States)

Slosárek, M

1977-01-01

It was the aim of the present communication to find a simple test for a reliable discrimination of Mycobacterium bovis BCG from Mycobacterium tuberculosis. A total of 26 BCG strains, out of them 10 Czechoslovak strains (2 lyophilized cultures of BCG of different batch, 6 strains isolated from abscesses of children after BCG-vaccination and 2 strains from fatal cases after BCG-vaccination) and 16 strains obtained from foreign laboratories, were used. Of the tested characteristics a combination of 3 tests, sensitivity to 1 microgram of 2-thiophene carbonylhydrazide (TCH), activity of 3 acylamidases (urease, nicotinamidase and pyrazinamidase) and a quantitative nitrate test, was found to be most advantageous. The Czechoslovak strains of Mycobacterium bovis BCG were fully sensitive to TCH, of the 3 acylamidases mentioned above only urease was positive and nitrate was reduced only little or not at all. On the other hand, strains of Mycobacterium tuberculosis were always resistant to TCH, had positive urease, nicotinamidase and pyrazinamidase and reduced nitrate very intensively.

Real-Time Measurement of Host Bioenergetics During Mycobacterium Tuberculosis Infection

Science.gov (United States)

2015-05-01

AWARD NUMBER: W81XWH-13-1-0149 TITLE: “Real-Time Measurement of Host Bioenergetics During Mycobacterium Tuberculosis Infection...successfully adapted metabolic flux analysis using a Seahorse XF96 metabolic flux analyzer to study Mycobacterium tuberculosis energy metabolism in an...Mycobacterium tuberculosis function. In: Systems Biology of Tuberculosis . Editors: J McFadden, D Beste and A Kierzek. 2013. Springer, New York, NY. 2

Treatment of radiostrontium uptake in man; Traitement de la fixation de radiostrontium chez l'homme; Lechenie posledstvij pogloshcheniya radioaktivnogo strontsiya u cheloveka; Tratamiento de la radiotoxemla humana causada por absorcion de radioestroncio

Energy Technology Data Exchange (ETDEWEB)

Volf, V. [Department of Ionizing Radiation Institute of Industrial Hygiene and Occupational Disease, Prague, CSSR (Czech Republic)

1963-02-15

'autres possibilites de traitement de premiere urgence de la contamination interne aigue par le radiostrontium chez l'homme. (author) [Spanish] En experimentos de corta duracion realizados con ratas, se ha modificado la absorcion del radioestroncio a traves de la pared intestinal por tratamiento oral con sulfato barico y fosfato calcico normal. Por el contrario, ha permanecido inalterada la absorcion del ''9''0Sr administrado con el agua de bebida a ratas sometidas a un regimen que comprendia sulfato barico. De ello se deduce que esta sustancia solo puede ser de alguna utilidad en un tratamiento de primeros auxilios. En dos casos de ligera contaminacion interna consecutiva a la inhalacion accidental de nitrato de {sup 90}Sr se ha administrado suliato barico (Skiabaryum Spoia) por via oral a los 10 y 30 minutos de ocurrir la inhalacion. La eficacia de este tratamiento se ha verificado en experimentos realizados con ratas en las mismas condiciones en que se produjeron los accidentes. En fecha mas reciente, se han efectuado experimentos analogos en varios enfermos de cancer para estudiar otras posibilidades que ofrece el tratamiento de primeros auxilios, administrado a raiz de una contaminacion interna aguda causada por el radioestroncio en el organismo humano. (author) [Russian] V neprodolzhitel'nykh ehksperimentakh na krysakh izuchalos' izmenenie prokhozhdeniya radiostrontsiya cherez kishechnuyu stenku pod vliyaniem skarmlivaniya sernokislogo bariya i normal'nogo fosfornokislogo kal'tsiya. Odnako u krys, soderzhashchikhsya na diete, vklyuchayushchej sernokislyj barij, ne obnaruzheno izmenenij vsasyvaniya strontsiya-90, kotoryj zhivotnye poluchali s pit'evoj vodoj. Sdelan vyvod, chto ehto veshchestvo mozhet primenyat'sya lish' dlya okazaniya pervoj pomoshchi. V dvukh sluchayakh legkogo vnutrennego zarazheniya posle sluchajnogo vdykhaniya nitrata strontsiya-90 sernokislyj barij (Skiabarij Spofa) davalsya vnutr' cherez 10 i 30 minut posle vdykhaniya strontsiya-90. Ehivnoot' ehtogo

Granulomatous lobular mastitis secondary to Mycobacterium fortuitum.

Science.gov (United States)

Kamyab, Armin

2016-12-16

Granulomatous lobular mastitis is a rare inflammatory disease of the breast of unknown etiology. Most present as breast masses in women of child-bearing age. A 29-year-old female presented with a swollen, firm and tender right breast, initially misdiagnosed as mastitis. Core needle biopsy revealed findings consistent with granulomatous lobular mastitis, and cultures were all negative for an infectious etiology. She was started on steroid therapy to which she initially responded well. A few weeks later she deteriorated and was found to have multiple breast abscesses. She underwent operative drainage and cultures grew Mycobacterium fortuitum . Granulomatous lobular mastitis is a rare inflammatory disease of the breast. The definitive diagnose entails a biopsy. Other causes of chronic or granulomatous mastitis should be ruled out, including atypical or rare bacteria such as Mycobacterium fortuitum . This is the first reported case of granulomatous mastitis secondary to Mycobacterium fortuitum . With pathologic confirmation of granulomatous mastitis, an infectious etiology must be ruled out. Atypical bacteria such as Mycobacterium fortuitum may not readily grow on cultures, as with our case. Medical management is appropriate, with surgical excision reserved for refractory cases or for drainage of abscesses.

Comparative molecular study of Mycobacterium tuberculosis strains, in times of antimicrobial drug resistance

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G. Varela

2005-03-01

Full Text Available Strains of Mycobacterium tuberculosis were compared using two DNA fingerprinting techniques: Restriction Fragment Length Polymorphism (RFLP and Double-Repetitive-Element-PCR (DRE-PCR. Two of these strains: IH1 (susceptible to isoniazid and IH2 (resistant to isoniazid were recovered from cases of pulmonary tuberculosis which occurred in two brothers who lived together. The first one was recognized on July 1999, and the second was diagnosed one year later. IH1 and IH2 showed the same pattern of bands with both molecular tests. These results suggest that single drug chemoprophylaxis may occasionally select resistant strains for that drug, which can eventually cause disease and be recognized through these tests. Strains IH3, IH4 and IH5 were obtained from sputum samples of 3 different patients, and intra-laboratory cross-contamination was suspected when it was realized that the 3 positive materials had been consecutively processed the same day by the same worker in the same biological safety cabinet. Again, the 3 strains revealed identical band patterns with RFLP and DRE-PCR, confirming the posed suspicion. The results with DRE-PCR were obtained after only 8 hours of work, without the need for subcultures. This procedure allows quick correction of treatment conducts, avoiding unnecessary exposure of people and bacteria to antimicrobial drugs.Se compararon cepas de Mycobacterium tuberculosis utilizando 2 procedimientos de ADN fingerprinting: polimorfismo de los fragmentos de restricción (RFLP y Double-Repetitive-Element-PCR (DRE-PCR. Dos de las cepas: IH1 (susceptible a isoniazida e IH2 (resistente a isoniazida se recuperaron a partir de casos de tuberculosis pulmonar que ocurrieron en dos hermanos convivientes. La primera fue aislada en julio de 1999 y la segunda un año después. IH1 e IH2 mostraron el mismo patrón de bandas por ambos procedimientos. Estos resultados sugieren que la quimioprofilaxis con una sola droga puede ocasionalmente

Evitar o efeito da vacinação BCG na detecção de infecção por Mycobacterium tuberculosis com um teste sanguíneo Avoiding the effect of BCG vaccination in detecting Mycobacterium tuberculosis infection with a blood test

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R. Diel

2007-01-01

Full Text Available O risco de progressão da infecção por Mycobacterium tuberculosis para tuberculose-doença é sobretudo elevado nos primeiros anos após a infecção, estimando-se que 50% dos casos de tuberculose-infecção desenvolvem doença nos dois anos seguintes. A investigação precoce dos contactos baseia-se neste facto, e o teste cutâneo de tuberculina tem sido largamente utilizado como a prova de rastreio, apesar da sua limitação na população vacinada com BCG, nas reacções cruzadas com micobactérias não tuberculosas e na anergia cutânea em grupos específicos. Nos presentes estudos avalia-se a maior especificidade (no primeiro e a maior sensibilidade (no segundo de um novo teste sanguíneo na detecção de tuberculose- infecção. Este teste laboratorial feito em uma única amostra de sangue do doente baseia-se na libertação de gama-interferão das células T CD4 do indivíduo infectado quando expostas a antigenes micobacterianos. A libertação de interferão é fortemente estimulada por antigenes do Mycobacterium tuberculosis ausentes no M. bovis e na grande maioria das restantes micobactérias - conferindo especificidade ao teste. Estes antigenes ESAT-6/ CFP10 (early secretory antigenic target 6/ culture filtrated protein 10 reagem com o sangue do indivíduo infectado, levando à libertação de gama-interferão medido pelos métodos ELISA ou ELISPOT, dependendo do fabricante, respectivamente - Quantiferon Gold; Cellestis Limited, Austrália; e T-Spot.TB, Oxford Immunotec, UK. No primeiro estudo referenciado, 369 pessoas da academia de Polícia alemã foram avaliadas para o risco de tuberculose-infecção após o contacto com militar a quem foi diagnosticada tuberculose pulmonar com presença de bacilos ácido-alcool resistentes 3+ em exames de expectoração, com posterior cultura positiva para Mycobacterium tuberculosis. Foi efectuado teste de Mantoux a todos os contactos, e aos que apresentavam o teste positivo (induração = 5mm

Mycobacterium intracellulare Infection Mimicking Progression of Scleroderma

DEFF Research Database (Denmark)

Krabbe, Simon; Engelhart, Merete; Thybo, Sören

2017-01-01

This case report describes a patient with scleroderma who developed Mycobacterium intracellulare infection, which for more than a year mimicked worsening of her connective tissue disorder. The patient was diagnosed with scleroderma based on puffy fingers that developed into sclerodactyly, abnormal......, unfortunately with significant scarring. Immunodeficiency testing was unremarkable. In summary, an infection with Mycobacterium intracellulare was mistaken for an unusually severe progression of scleroderma....

A PULMONARY INFECTION CAUSED BY MYCOBACTERIUM PEREGRINUM– A CASE REPORT.

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Tatina T. Todorova

2015-12-01

Full Text Available Mycobacterium peregrinum is a member of the group of rapidly growing Nontuberculous Mycobacteria (NTM. It can be found in high frequency in natural and laboratory environments and is considered to be uncommonrare pathogen for both immunocompetent and immunosuppressed individuals. Currently, pulmonary infections caused by Mycobacterium peregrinum are unusual and diagnosed only in limited number of cases. Here, we present a clinical case of elderly man (72 years with 1 month history of non-specific respiratory symptomatic. The patient was without underlying immunosuppressive condition or lung disease. Chest X-ray demonstrated persistent pleural effusion, opacities and cavitations in the right lobe. One of the sputum culturesgrewa rapidly growing mycobacterium and the isolated strain was found to be Mycobacterium peregrinumas identified by molecular genetic detection (PCR and DNA strip technology. To our knowledge, this is the third case in the world to report Mycobacterium peregrinumas a possible causative agent of pulmonary infection.

Revival and emended description of 'Mycobacterium paraffinicum' Davis, Chase and Raymond 1956 as Mycobacterium paraffinicum sp. nov., nom. rev.

Science.gov (United States)

Toney, Nadege; Adekambi, Toidi; Toney, Sean; Yakrus, Mitchell; Butler, W Ray

2010-10-01

The omission of the name 'Mycobacterium paraffinicum' from the Approved Lists of Bacterial Names was due to phenotypic confusion surrounding a close relationship with Mycobacterium scrofulaceum. Correspondingly, 'M. paraffinicum' strains grew slowly in > 7 days, stained acid-alcohol-fast and produced yellow-pigmented, smooth, waxy colonies in the dark at an optimal temperature of 35°C. However, 'M. paraffinicum' strains demonstrated no activity for urease, nicotinamidase or pyrazinamidase and lacked growth at 42°C, unlike M. scrofulaceum. The mycolic acid pattern, as determined by HPLC, clustered 'M. paraffinicum' with M. scrofulaceum, Mycobacterium avium and Mycobacterium parascrofulaceum. Strains were fully susceptible to linezolid, rifabutin, clarithromycin and amikacin. Examination of the historical reference strain of 'M. paraffinicum', ATCC 12670, and five additional isolates using comparative studies with 16S rRNA, hsp65 and rpoB gene and concatenated sequences showed that they formed a tight taxonomic group that was distinct from similar non-tuberculous mycobacteria. Multilocus enzyme electrophoresis (MEE) analysis confirmed a close association of the five additional isolates with the reference strain of 'M. paraffinicum' with a genetic distance of 0.12 and showed that all six strains were distinct from other closely related species. These genetic results provided unambiguous evidence of the uniqueness of this slowly growing, scotochromogenic species and supported the revival of the name as Mycobacterium paraffinicum (ex Davis, Chase and Raymond 1956) sp. nov., nom. rev. We propose the previously deposited reference strain ATCC 12670(T) =DSM 44181(T) =NCIMB 10420(T), located in collections worldwide, as the type strain.

Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul

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Daniela de O. Cazola

2015-02-01

Full Text Available Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping. Tecidos de 13 bovinos positivos, oriundos de diferentes municípios do estado, foram cultivados em meio de Stonebrink. As colônias resultantes foram submetidas à coloração de Ziehl-Neelsen e todos os isolados apresentaram características tintoriais de BAAR. Os 13 isolados de BAAR foram identificados por PCR multiplex (mPCR. O gene hsp65 foi alvo para identificação de Mycobacterium spp, a sequência de inserção IS6110 foi alvo para identificação de complexo Mycobacterium tuberculosis (CMT e a região rvd1rv2031c foi explorada para detecção de M. bovis. Os isolados micobacterianos foram genotipados pela técnica de spoligotyping. Dos 13 bovinos, sete tinham pelo menos uma lesão sugestiva de tuberculose em linfonodos retrofaríngeos, parotídeos e pulmonares ou no pulmão, e em seis não foram encontradas lesões visíveis sugestivas da doença. Na mPCR, 11/13 (84,6% isolados foram positivos para Mycobacterium spp; 8/13 (61,5% positivos para CMT e 7/13 (53,8% positivos para M. bovis. Com base no spoligotyping, oito isolados de BAAR foram agrupados dentro de três diferentes agrupamentos de genótipos e uma amostra remanescente apresentou perfil único, sendo quatro isolados com padrão de espoligotipo SB0121, dois SB1145, dois SB0881 e um SB0140. A técnica de spoligotyping demonstrou que há diversidade genética entre os espoligotipos presentes no estado de Mato Grosso do Sul, embora predomine o perfil SB0121

Mycobacterium avium ssp. paratuberculosis (MAP): Identificação água e fatores de risco para a presença em amostras de biópsias intestinais

OpenAIRE

Braga, Isis de Freitas Espeschit

2015-01-01

Mycobacterium avium ssp. paratuberculosis (MAP) é o agente etiológico da doença de Johne ou paratuberculose, enterite granulomatosa crônica caracterizada por diarreia persistente e perda de peso progressiva que acomete ruminantes. Pode também ser isolado a partir de amostras intestinais de pacientes humanos, com doenças intestinais, principalmente portadores da doença de Crohn. Essa é uma doença de etiologia desconhecida, que se caracteriza por inflamação crônica, focal, assimétrica transmura...

Mycobacterium lutetiense sp. nov., Mycobacterium montmartrense sp. nov. and Mycobacterium arcueilense sp. nov., members of a novel group of non-pigmented rapidly growing mycobacteria recovered from a water distribution system.

Science.gov (United States)

Konjek, Julie; Souded, Sabiha; Guerardel, Yann; Trivelli, Xavier; Bernut, Audrey; Kremer, Laurent; Welte, Benedicte; Joyeux, Michel; Dubrou, Sylvie; Euzeby, Jean-Paul; Gaillard, Jean-Louis; Sapriel, Guillaume; Heym, Beate

2016-09-01

From our recent survey of non-pigmented rapidly growing mycobacteria in the Parisian water system, three groups of isolates (taxons 1-3) corresponding to possible novel species were selected for taxonomic study. The three taxa each formed creamy white, rough colonies, had an optimal growth temperature of 30 °C, hydrolyzed Tween 80, were catalase-positive at 22 °C and expressed arylsulfatase activity. All three were susceptible to amikacin, ciprofloxacin and tigecycline. The three taxa produced specific sets of mycolic acids, including one family that has never previously been described, as determined by thin layer chromatography and nuclear magnetic resonance. The partial rpoB sequences (723 bp) showed 4-6 % divergence from each other and more than 5 % differences from the most similar species. Partial 16S rRNA gene sequences showed 99 % identity within each species. The most similar sequences for 16S rRNA genes (98-99 % identity over 1444-1461 bp) were found in the Mycobacterium fortuitum group, Mycobacterium septicum and Mycobacterium farcinogenes. The three taxa formed a new clade (bootstrap value, 99 %) on trees reconstructed from concatenated partial 16S rRNA, hsp65 and rpoB sequences. The above results led us to propose three novel species for the three groups of isolates, namely Mycobacterium lutetiense sp. nov. [type strain 071T=ParisRGMnew_1T (CIP 110656T=DSM 46713T)], Mycobacterium montmartrense sp. nov. [type strain 196T=ParisRGMnew_2T (CIP 110655T=DSM 46714T)] and Mycobacteriu marcueilense sp. nov. [type strain of 269T=ParisRGMnew_3T (CIP 110654T=DSM 46715T)].

Bone marrow infection with mycobacterium fortuitum in a diabetic patient

International Nuclear Information System (INIS)

Satti, L.; Abbasi, S.; Sattar, A.; Ikram, A.; Manzar, M.A.; Khalid, M.M.

2011-01-01

Incidence and prevalence of Mycobacterium fortuitum infection vary greatly by location and death is very rare except in disseminated disease in immunocompromised individuals. We present what we believe is the first case of bone marrow infection with Mycobacterium fortuitum in an HIV negative patient. Bone marrow examination revealed presence of numerous acid fast bacilli which were confirmed as Mycobacterium fortuitum on culture and by molecular analysis. Patient was managed successfully with amikacin and ciprofloxacin. (author)

Identificación de Mycobacterium sp., en una población de tortugas morrocoy (Geochelone carbonaria en cautiverio y en su entorno, en un zoológico en la Sabana de Bogotá

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Ángela Natalia Agudelo

2008-06-01

Full Text Available En un Zoológico de la Sabana de Bogotá, se presentó alta mortalidad de aves por tuberculosis aviar, en un encierro en el cual habitaban dos clases de animales diferentes: reptiles y aves. Se buscó establecer la presencia del Mycobacterium sp, por medio de la identificación molecular (PCR-PRA, en una población de 19 tortugas Morrocoy en cautiverio en el Zoológico mencionado anteriormente. Se procedió a tuberculinizar a todas las tortugas, las cuales resultaron negativas y se recolectaron muestras de materia fecal y muestras ambientales (agua y suelo y se cultivaron en medios OK/MSTA, LJ y OK respectivamente realizando baciloscopia para cada una de las muestras. De la muestras de materia fecal sólo cuatro fueron positivas a baciloscopia y de nueve muestras ambientales (suelo (n=7, agua (n=2, cinco fueron positivas (suelo (n=4, agua (n=1; en cuanto al crecimiento fueron negativas todas las de materia fecal de las tortugas Morrocoy. De las muestras ambientales (suelo, agua crecieron cinco y una muestras respectivamente. Adicionalmente se obtuvo muestras de la necropsia de una tortuga Icotea, (tejido, orina y absceso y sólo hubo crecimiento de la muestra de absceso. De la muestra de absceso se identificó Mycobacterium gordonae tipo 3, de las de suelo se obtuvo Mycobacterium avium tipo 3 y en el de agua se obtuvo Mycobacterium fortuitum tipo 1. Los hallazgos sugieren la necesidad de una vigilancia continua, que permita la identificación de la presencia de micobacterias; por medio de pruebas de laboratorio apropiadas (baciloscopia, cultivo, pruebas bioquímicas y moleculares; ya que se debe evitar que las tortugas sigan siendo parte de un ciclo epidemiológico de transmisión como portadores sanos y el contacto con los humanos debe darse sólo cuando sea estrictamente necesario, aplicando normas de bioseguridad.

A Case of False-Positive Mycobacterium tuberculosis Caused by Mycobacterium celatum

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Edward Gildeh

2016-01-01

Full Text Available Mycobacterium celatum is a nontuberculous mycobacterium shown to cause symptoms similar to pulmonary M. tuberculosis. Certain strains have been shown to cross-react with the probes used to detect M. tuberculosis, making this a diagnostic challenge. We present a 56-year-old gentleman who developed signs and symptoms of lung infection with computed tomography scan of the chest showing right lung apex cavitation. Serial sputum samples were positive for acid-fast bacilli and nucleic acid amplification testing identified M. tuberculosis ribosomal RNA, resulting in treatment initiation. Further testing with high performance liquid chromatography showed a pattern consistent with M. celatum. This case illustrates the potential for M. celatum to mimic M. tuberculosis in both its clinical history and laboratory testing due to the identical oligonucleotide sequence contained in both. An increasing number of case reports suggest that early reliable differentiation could reduce unnecessary treatment and public health intervention associated with misdiagnosed tuberculosis.

Mycobacterium marinum infections in Denmark from 2004 to 2017

DEFF Research Database (Denmark)

Holden, Inge K.; Kehrer, Michala; Andersen, Aase B.

2018-01-01

Mycobacterium marinum (M. marinum) is a slowly growing nontuberculous mycobacterium. The incidence of M. marinum infections in Denmark is unknown. We conducted a retrospective nationwide study including all culture confirmed cases of M. marinum from 2004 to 2017 in Denmark. All available medical ...

Caracterización de accidentes de trabajo por caída de objetos en empresas del sector hidrocarburos

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Gloria A. Jaramillo

2014-12-01

Full Text Available En una multinacional del sector de hidrocarburos prestadora de servicios de perforación y workover, que opera en las zonas de Tolima y Santander Colombia, se pudo desarrollar una investigación con el objetivo de describir la situación de accidentalidad causada por caída de objetos que se presenta en el área de taladros de perforación. La información se recolectó de los reportes de accidentes e incidentes que reposaban en la base de operaciones en Sopó entre el periodo 2010 y 2011. Al analizar los hallazgos se pudo evidenciar que son cuatro los agentes causales más significativos, dentro de los cuales están los denominados Golpes por objetos, ocupando un 68%; atrapado por o entre un objeto con 41%; caída de personas con un 30% y caída de objetos con 21 %. Este último hallazgo se deriva por la actividad propia de la perforación por la manipulación de llaves hidráulicas, objetos adicionales de acero, martillos, entre otros y su causa básica fue la falta de supervisión, liderazgo y/o planificación con 39%, seguida de procedimientos de trabajo inadecuados con el 22% y el mantenimiento inadecuado con el 11%, lo que concluye que en su mayoría son errores humanos.

The frequency of nasal injury in newborns due to the use of continuous positive airway pressure with prongs La frecuencia de lesión nasal en neonatos causada por la presión positiva continua en las vías aéreas con prongs Frequência de lesão nasal em neonatos por uso de pressão positiva contínua nas vias aéreas com pronga

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Renata Medeiros do Nascimento

2009-08-01

Full Text Available Continuous positive airway pressure (CPAP with prongs is the ventilatory support most used in newborns. Nasal injuries are complications that may arise due to the prolonged use of this device. This study aimed to determine the frequency of nasal injuries in newborns through the use of continuous positive airway pressure with prongs. A convenience sample composed of hospitalized newborns using prongs for more than two days was used. Data were collected through a structured form. Lesions were observed in all newborns, which were classified as: mild (79.6%, moderate (19.7% and severe (0.7%. The conclusion is that the use of prongs for more than two days represents a risk factor for the lesions to develop.La presión positiva continua en las vías aéreas con prongs es la asistencia ventiladora más utilizada en neonatología. Las lesiones nasales son complicaciones que pueden surgir debido al uso prolongado de ese dispositivo. Este estudio tuvo por objetivo determinar la frecuencia de las lesiones nasales en neonatos causadas debido al uso de presión positiva continua en las vías aéreas utilizando un prong. El muestreo fue por conveniencia, constituido de recién nacidos internados y usando prongs, y se excluyeron aquellos que utilizaban el dispositivo por un período inferior a dos días. Los datos fueron recolectas a través de un formulario estructurado. Se observaron lesiones en todos los recién nacidos, clasificadas en: ligera (79,6%, moderada (19,7% y severa (0,7%. Se concluyó que el tiempo de permanencia de las prongs, por más de dos días, es un factor de riesgo para la evolución de las etapas de las lesiones nasales.A pressão positiva contínua nas vias aéreas com pronga é a assistência ventilatória mais utilizada em neonatologia. Lesões nasais são complicações que podem surgir pelo uso prolongado desse dispositivo. Este estudo objetivou determinar a frequência de lesões nasais em neonatos por uso de pressão positiva

Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization.

Science.gov (United States)

Gcebe, Nomakorinte; Rutten, Victor; Pittius, Nicolaas Gey van; Naicker, Brendon; Michel, Anita

2017-04-01

Non-tuberculous mycobacteria (NTM) are ubiquitous in the environment, and an increasing number of NTM species have been isolated and characterized from both humans and animals, highlighting the zoonotic potential of these bacteria. Host exposure to NTM may impact on cross-reactive immune responsiveness, which may affect diagnosis of bovine tuberculosis and may also play a role in the variability of the efficacy of Mycobacterium bovis BCG vaccination against tuberculosis. In this study we characterized 10 NTM isolates originating from water, soil, nasal swabs of cattle and African buffalo as well as bovine tissue samples. These isolates were previously identified during an NTM survey and were all found, using 16S rRNA gene sequence analysis to be closely related to Mycobacterium moriokaense. A polyphasic approach that included phenotypic characterization, antibiotic susceptibility profiling, mycolic acid profiling and phylogenetic analysis of four gene loci, 16S rRNA, hsp65, sodA and rpoB, was employed to characterize these isolates. Sequence data analysis of the four gene loci revealed that these isolates belong to a unique species of the genus Mycobacterium. This evidence was further supported by several differences in phenotypic characteristics between the isolates and the closely related species. We propose the name Mycobacterium malmesburyense sp. nov. for this novel species. The type strain is WCM 7299T (=ATCC BAA-2759T=CIP 110822T).

Vigilancia de serotipos en infecciones invasivas por Haemophilus influenzae en la Argentina en la era de la vacuna conjugada contra el serotipo b durante el período 2005-2010

OpenAIRE

Efron, Adriana M.; Moscoloni, María A.; Reijtman, Vanesa R.; Regueira, Mabel

2013-01-01

La introducción de la vacuna contra Haemophilus influenzae tipo b en los programas de inmunización de muchos países produjo una reducción marcada en la incidencia de enfermedad invasiva causada por este serotipo y en su portación y un incremento de otros tipos capsulares y de aislamientos no capsulados. Se estudiaron 313 aislamientos de H. influenzae recuperados de sitio estéril, provenientes de pacientes pediátricos y adultos con enfermedad invasiva atendidos en 90 hospitales de la Red Nacio...

Complete Genome Sequence of Mycobacterium phlei Type Strain RIVM601174

KAUST Repository

Abdallah, A. M.; Rashid, M.; Adroub, S. A.; Arnoux, M.; Ali, Shahjahan; van Soolingen, D.; Bitter, W.; Pain, Arnab

2012-01-01

Mycobacterium phlei is a rapidly growing nontuberculous Mycobacterium species that is typically nonpathogenic, with few reported cases of human disease. Here we report the whole genome sequence of M. phlei type strain RIVM601174.

Complete Genome Sequence of Mycobacterium phlei Type Strain RIVM601174

KAUST Repository

Abdallah, A. M.

2012-05-24

Mycobacterium phlei is a rapidly growing nontuberculous Mycobacterium species that is typically nonpathogenic, with few reported cases of human disease. Here we report the whole genome sequence of M. phlei type strain RIVM601174.

Mycobacterium abscessus skin infection after tattooing - Case report*

Science.gov (United States)

de Sousa, Pétra Pereira; Cruz, Rossilene Conceição da Silva; Schettini, Antonio Pedro Mendes; Westphal, Danielle Cristine

2015-01-01

Mycobacterium abscessus is a rapidly growing mycobacterium that has been affecting people undergoing invasive procedures, such as videosurgery and mesotherapy. This bacterium has global distribution, being found in numerous niches. The frequency of published reports of infection by rapidly growing mycobacteria associated with tattooing procedures has increased in recent years. However, in Brazil there were no case reports of M. abscessus after tattooing in the literature until now. In this paper, we describe the case of a patient with a nine-month history of lesion on a tattoo site. The diagnosis of infection with Mycobacterium abscessus was established by correlation between dermatological and histopathological aspects, culture and molecular biology techniques. The patient had significant improvement of symptoms with the use of clarithromycin monotherapy. PMID:26560222

Beta-lactamases of Mycobacterium tuberculosis and Mycobacterium kansasii.

Science.gov (United States)

Segura, C; Salvadó, M

1997-09-01

Re-emergence of infectious diseases caused by mycobacteria as well as the emergence of multiresistant strains of Mycobacterium has promoted the research on the use of beta-lactames in the treatment of such diseases. Mycobacteria produce beta-lactamases: M. tuberculosis produces a wide-spectrum beta-lactamase whose behaviour mimicks those of Gram-negative bacteria. M. kansasii produces also beta-lactamase which can be inhibited by clavulanic acid. An overview on beta-lactamases from both species is reported.

Toxoplasma gondii myelitis in a patient with adult T-cell leukemia-lymphoma Mielite por Toxoplasma gondii em um paciente com leucemia-linfoma de células T do adulto

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ELVES MACIEL

2000-12-01

Full Text Available Adult T cell leukemia-lymphoma (ATL caused by HTLV-I may be associated with severe immunosupression and several opportunistic infections. Toxoplasmic encephalitis is a common central nervous system opportunistic infection in severely immunosupressed patients, however spinal cord involvement by this parasite is rare. In this paper, we report a case of toxoplasmic myelitis in a patient with ATL.Leucemia de células T do adulto (ATL, causada pelo HTLV-I, pode estar associada com imunossupressão severa e muitas infecções oportunistas. Encefalite por toxoplasmose é uma infecção oportunista do sistema nervoso central em pacientes imunossuprimidos, no entanto o envolvimento da medula espinal por este parasita é raro. Neste artigo, apresentamos um caso de mielite em um paciente com ATL.

Mycobacterium mageritense Parotitis in an Immunocompetent Adult.

Science.gov (United States)

Okabe, Taro; Sasahara, Teppei; Suzuki, Jun; Onishi, Tsubasa; Komura, Masayoshi; Hagiwara, Shigehiro; Suzuki, Hiromichi; Morisawa, Yuji

2018-03-01

Mycobacterium mageritense , a rapidly growing mycobacterium, is a rare clinical pathogen. Furthermore, parotitis due to non-tuberculosis mycobacterium is very rare in adults. Herein, we report the first case of M. mageritense parotitis in an immunocompetent adult. A 40-year-old man presented with swelling in a left parotid lesion. He was diagnosed with parotitis. The culture from the parotid abscess grew M. mageritense . He was unsuccessfully treated with levofloxacin monotherapy. Trimethoprim-sulfamethoxazole was added, leading to some clinical response; however, the erythema persisted despite 14 months of antibiotic therapy. Subsequently, the skin lesion was surgically removed. The antibiotic treatment was ceased a week after surgery as the postoperative course was uneventful and the lesion had improved. No recurrence was noted at 7 months after surgery. Although extremely rare, M. mageritense can cause parotitis in immunocompetent adults, and may not be sufficiently treated with antibiotics alone.

Prevalência, avaliação clínica e imunológica de cães (Canis familiaris) (Linnaeus, 1758) naturalmente infectados por Leishmania (Leishmania) chagasi (Cunha & Chagas, 1937) provenientes do município de Tamandaré, região litoral Sul do Estado de Pernambuco, Brasil

OpenAIRE

Marco Antônio Granja Barbosa

2009-01-01

A Leishmaniose Visceral Canina (LVC) no Brasil é causada por Leishmania (Leishmania) chagasi a qual é relatada em várias regiões. O objetivo desta pesquisa foi verificar a prevalência, analisar a correlação espacial entre a Leishmaniose Visceral Canina e fatores ambientais, avaliar e comparar os níveis de citocinas (IL-12, IFN-γ, IL-2 e IL-4) expressões por PCR em tempo real em diferentes tecidos/órgãos, determinar os padrões bio-químicos e hematológicos em cães naturalmente infectados p...

Microepidemia familiar por Trichophyton tonsurans Trichophyton tonsurans in a family microepidemic

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Tânia Pereira Salci

2011-10-01

Full Text Available Trichophyton tonsurans é um fungo dermatófito antropofílico de alta transmissibilidade que invade tecidos queratinizados. Relatamos um caso de microepidemia familiar causada por esse dermatófito no qual, apesar das ótimas condições de higiene, o fungo se manteve viável por vários anos, disseminando-se para todos os membros da família. A hipótese de que estivesse sendo mantido na residência da família foi confirmada após análise de amostras do domicílio, em que foram isoladas e identificadas culturas puras do fungo. Após o diagnóstico, a residência foi desinfetada e todos os membros da família receberam tratamento oral concomitantemente.Trichophyton tonsurans is a highly transmissible anthropophilic dermatophyte fungus, which invades keratinized tissues. This study reports a case of family microepidemic caused by this dermato phyte. Despite their excellent hygiene conditions, it remained active for several years, spreading to all family members. The hypothesis that the fungus was being kept alive in the family home was confirmed after samples collected from it were analyzed. Pure cultures of the fungus were isolated and identified. After diagnosis, the house was disinfected with concomitant oral treatment for all family members.

Mycobacterium aquaticum sp. nov., a rapidly growing species isolated from haemodialysis water.

Science.gov (United States)

Hashemi Shahraki, Abdolrazagh; Trovato, Alberto; Droz, Sara; Haidarieh, Parvin; Borroni, Emanuele; Mirsaeidi, Mehdi; Mannino, Roberta; Hashemzadeh, Mohamad; Mariottini, Alessandro; Cirillo, Daniela Maria; Tortoli, Enrico

2017-09-01

The characterization of five Iranian isolates, four from hospital haemodialysis water and one from the sputum of a patient, led to the detection of a novel mycobacterium species. The strains were characterized by mucoid colonies developing in 3-5 days at temperatures ranging from 25 to 37 °C. The biochemical test pattern was unremarkable while the HPLC profile of mycolic acids resembled that of Mycobacterium fortuitum. The sequences of three major housekeeping genes (16S rRNA, hsp65 and rpoB) were unique and differed from those of any other mycobacterium. Mycobacterium brisbanense, which is the species that shared the highest 16S rRNA gene sequence similarity (99.03 %), was distinct, as shown by the average nucleotide identity and by the genome to genome distance values (91.05 and 43.10 %, respectively). The strains are thus considered to represent a novel species of the genus Mycobacterium, for which the name Mycobacterium aquaticum sp. nov. is proposed. The type strain is RW6T (=DSM 104277T=CIP111198T).

Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre

OpenAIRE

Portis, Igor Godinho

2015-01-01

Paracoccidioidomicose é a micose sistêmica mais prevalente da América Latina, causada por fungos do gênero Paracoccidioides spp. O fungo cresce como micélio no ambiente saprofítico, sendo a infecção causada por propágulos de micélio e ou conídios, que se diferenciam na forma leveduriforme no hospedeiro. O cobre é um micronutriente essencial para eucariotos e bactérias, de extrema importância a várias proteínas, sendo cofator de muitas enzimas e utilizado em diversos proces...

Principais doenças de Anonáceas no Brasil: descrição e controle

OpenAIRE

Nilton Tadeu Vilela Junqueira; Keize Pereira Junqueira

2014-01-01

Várias doenças podem afetar folhas, caules, flores e frutos de gravioleira, pinheira e atemoia em diferentes estádios de seus desenvolvimentos. Geralmente, as doenças mais importantes são causadas por fungos durante o florescimento e a frutificação. Também podem ocorrer murchas ou podridões de raízes, causadas por nematoides e patógenos do solo. A antracnose (Colletotrichum gloeosporioides), murcha ou podridões de raízes (Rhizoctonia solani, Cylindrocladium clavatum, Phytophthora sp., Pythium...

Mycobacterium marinum kan være vanskelig at diagnosticere

DEFF Research Database (Denmark)

Lønnberg, Ann Sophie; Seersholm, Niels; Nielsen, Signe Ledou

2012-01-01

The diagnosis of cutaneous Mycobacterium marinum infection is often delayed for months after presentation. In this case the diagnosis and correct treatment was delayed for ten months resulting in possible irreversible damage to the patient's infected finger. The main reason for the delay is lack...... of knowledge of the mycobacterium....

An orphan gyrB in the Mycobacterium smegmatis genome

Indian Academy of Sciences (India)

DNA gyrase is an essential topoisomerase found in all bacteria. It is encoded by gyrB and gyrA genes. These genes are organized differently in different bacteria. Direct comparison of Mycobacterium tuberculosis and Mycobacterium smegmatis genomes reveals presence of an additional gyrB in M. smegmatis flanked by ...

Chronic breast abscess due to Mycobacterium fortuitum: a case report

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MacNeill Fiona A

2011-05-01

Full Text Available Abstract Introduction Mycobacterium fortuitum is a rapidly growing group of nontuberculous mycobacteria more common in patients with genetic or acquired causes of immune deficiency. There have been few published reports of Mycobacterium fortuitum associated with breast infections mainly associated with breast implant and reconstructive surgery. Case presentation We report a case of a 51-year-old Caucasian woman who presented to our one-stop breast clinic with a two-week history of left breast swelling and tenderness. Following triple assessment and subsequent incision and drainage of a breast abscess, the patient was diagnosed with Mycobacterium fortuitum and treated with antibiotic therapy and surgical debridement. Conclusion This is a rare case of a spontaneous breast abscess secondary to Mycobacterium fortuitum infection. Recommended treatment is long-term antibacterial therapy and surgical debridement for extensive infection or when implants are involved.

Mycobacterium shigaense Causes Lymph Node and Cutaneous Lesions as Immune Reconstitution Syndrome in an AIDS Patient: The Third Case Report of a Novel Strain Non-tuberculous Mycobacterium

Science.gov (United States)

Koizumi, Yusuke; Shimizu, Kaoru; Shigeta, Masayo; Minamiguchi, Hitoshi; Hodohara, Keiko; Andoh, Akira; Tanaka, Toshihide; Chikamatsu, Kinuyo; Mitarai, Satoshi; Mikamo, Hiroshige

2016-01-01

A 40-year-old man complaining of progressive body weight loss was diagnosed to have acquired immunodeficiency syndrome. Within 2 weeks after the initiation of combination antiretroviral therapy, he developed fever, massive cervical lymphadenopathy and a protruding subcutaneous abscess. A lymph node biopsy and abscess drainage revealed non-caseous granuloma and mycobacterium. The mycobacterium belonged to Runyon II group, but it showed no matches to any previously reported species. According to sequence analyses, the strain was identified as Mycobacterium shigaense. After six months of antimycobacterial treatment, the lesions were all successfully cured. This is the third case report of the novel mycobacterium, M. shigaense, presenting in associatioin with immune reconstitution syndrome. PMID:27853087

Desarrollo de una metodología para el estudio de las inundaciones causadas por encharcamiento de agua de lluvia en zonas urbanas relativamente planas. Area de estudio: Montería (Colombia

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Padilla Agamez, Manuel

2015-06-01

Full Text Available In this paper a methodology for the analysis of the relationship between rainfall and flooding caused by ponding of rainfall water in urban area of Monteria (Colombia is developed. Rainfall water excesses over the soil were estimated from field measurements of the soil infiltration capacity for different soil units of the studied zone. The results obtained are presented in form of statistical correlations for calculating water rainfall excesses over the soils and maps of susceptibility and vulnerability for floods. The maps were used for a brief socioeconomic discussion of the effects of flood events in the selected zone.En el presente trabajo se desarrolla una metodología para el análisis de la relación existente entre las precipitaciones y las inundaciones causadas por encharcamiento de agua de lluvia en la zona urbana de Montería (Colombia. Se realizaron mediciones en campo de la capacidad de infiltración de agua para diferentes unidades de suelos de la zona de estudio, con el fin de estimar los excesos de agua de lluvia sobre el suelo. Los resultados obtenidos se presentan en forma de correlaciones estadísticas para el cálculo de los excesos de agua de lluvia y mapas de susceptibilidad y vulnerabilidad a las inundaciones, mediante los cuales se hace una breve discusión socioeconómica de los efectos de esta clase de inundaciones en la zona de estudio. [fr] Dans le présent travail est analysé la relation existant entre les précipitations et des inondations par accumulation d eau pluie sur le sol dans la zone urbaine de Montería (Colombie. Avec la réalisation de mesures en champ de la capacité d’infiltration pour différents unities de sol de la zone d’étude, on a estimé les excès d’eau pluie sur le sol. Les résultats obtenus sont présentés sous la forme de corrélations statistiques pour le calcul des excès d’eau pluie et de cartes de susceptibilité et vulnérabilité aux inondations. Les cartes ont été employ

Performance Assessment of the CapitalBio Mycobacterium Identification Array System for Identification of Mycobacteria

Science.gov (United States)

Liu, Jingbo; Yan, Zihe; Han, Min; Han, Zhijun; Jin, Lingjie; Zhao, Yanlin

2012-01-01

The CapitalBio Mycobacterium identification microarray system is a rapid system for the detection of Mycobacterium tuberculosis. The performance of this system was assessed with 24 reference strains, 486 Mycobacterium tuberculosis clinical isolates, and 40 clinical samples and then compared to the “gold standard” of DNA sequencing. The CapitalBio Mycobacterium identification microarray system showed highly concordant identification results of 100% and 98.4% for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM), respectively. The sensitivity and specificity of the CapitalBio Mycobacterium identification array for identification of Mycobacterium tuberculosis isolates were 99.6% and 100%, respectively, for direct detection and identification of clinical samples, and the overall sensitivity was 52.5%. It was 100% for sputum, 16.7% for pleural fluid, and 10% for bronchoalveolar lavage fluid, respectively. The total assay was completed in 6 h, including DNA extraction, PCR, and hybridization. The results of this study confirm the utility of this system for the rapid identification of mycobacteria and suggest that the CapitalBio Mycobacterium identification array is a molecular diagnostic technique with high sensitivity and specificity that has the capacity to quickly identify most mycobacteria. PMID:22090408

Environmental Mycobacterium avium subsp. paratuberculosis hosted by free-living amoebae

Science.gov (United States)

Mycobacterium avium subsp. paratuberculosis is responsible for paratuberculosis in animals. This disease, leading to an inflammation of the gastrointestinal tract, has a high impact on animal health and an important economic burden. The environmental life cycle of Mycobacterium avium subsp. paratube...

Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954

KAUST Repository

Ho, Y. S.

2012-10-26

Mycobacterium vaccae is a rapidly growing, nontuberculous Mycobacterium species that is generally not considered a human pathogen and is of major pharmaceutical interest as an immunotherapeutic agent. We report here the annotated genome sequence of the M. vaccae type strain, ATCC 25954.

Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954

KAUST Repository

Ho, Y. S.; Adroub, S. A.; Abadi, Maram; Al Alwan, B.; Alkhateeb, R.; Gao, G.; Ragab, A.; Ali, Shahjahan; van Soolingen, D.; Bitter, W.; Pain, Arnab; Abdallah, A. M.

2012-01-01

Mycobacterium vaccae is a rapidly growing, nontuberculous Mycobacterium species that is generally not considered a human pathogen and is of major pharmaceutical interest as an immunotherapeutic agent. We report here the annotated genome sequence of the M. vaccae type strain, ATCC 25954.

Mastite com septicemia em caninos causada por Staphylococcus intermedius

OpenAIRE

Borowsky, Luciane; Driemeier, David; Rozza, Daniela; Cardoso, Marisa Ribeiro de Itapema

2003-01-01

Os casos de mastite em caninos ocorrem mais comumente no período pós-parto ou como complicação de casos de pseudociese. Os microrganismos mais isolados nestes casos têm sido enterobactérias, estreptococos e estafilococos. Neste relato, são descritos três casos de cães com mastite aguda que evoluíram para óbito. Os animais apresentavam vômitos, abatimento, dificuldade respiratória e hálito urêmico. Na necropsia havia mastite gangrenosa e abscessos mamários nas mamas abdominais caudais e inguin...

Papel de la proteína supresora de la señalización por citocinas-3 (SOCS 3 en la resistencia a la hormona de crecimiento inducida por malnutrición.

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Adriana Umaña

2003-09-01

Full Text Available La nutrición es un regulador importante de las acciones de la hormona de crecimiento (GH. Se ha demostrado que el déficit de nutrientes induce un estado de resistencia a la hormona, en el cual están involucrados, entre otros factores, alteraciones post-receptor en la vía de señalización, pero se desconocen los mecanismos responsables. En este trabajo se investigó la participación de algunos miembros de la familia de proteínas supresoras de la señalización por citocinas (SOCS en la resistencia causada por malnutrición, que inhibe la activación de la señalización a través de Janus cinasa 2/transductor de señal y activador de la transcripción 5 (JK2/STAT5. Se estudiaron los cambios en la expresión génica del receptor de GH (RGH, IGF-I y SOCS3 en el hígado de ratas alimentadas con una dieta baja en proteína (8% y estimuladas con GH. La restricción en el consumo de proteína disminuyó significativamente (p

Miopatia pseudo-hipertrófica por cisticercose: registro de caso

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Maria Ines de Vilhena Lana-Peixoto

1985-12-01

Full Text Available O caso de uma paciente com aumento de volume das pernas, dor discreta e dificuldade para deambular é descrito. A eletromiografia mostrou potenciais de unidades motoras gigantes com padrão de interferência completo. A biópsia dos gastrocnêmius direito e esquerdo revelou presença de um cisticerco além de hipertrofia das fibras musculares e infiltrado inflamatório. A revisão da literatura demonstra que apenas 12 casos de miopatia pseudo-hipertrófica causada por cisticercos foram relatados e que a incidência é duas vezes maior no sexo masculino do que no feminino. Em cerca de metade dos casos há história de epilepsia. Dor e fraqueza muscular discretas são frequentes. Em geral a pseudo-hipertrofia ocorre nas cinturas escapular e pélvica simultaneamente. Miotonia é achado raro e nódulos subcutâneos estão presentes na maioria dos pacientes. Nosso paciente difere dos outros relatados na literatura por apresentar pseudo-hipertrofia restrita às pernas, principalmente à esquerda, com eletromiografia revelando potenciais de unidades motoras gigantes. A patogênese desta condição é discutida e o diagnóstico diferencial deve ser feito com distrofia muscular pseudo-hipertrófica, triquinose, miotonia congênita, hipotireoidismo, amiloidose e a forma juvenil da glicogenose do tipo I (doença de Pompe.

Evaluation of Genetic Pattern of Non-Tuberculosis Mycobacterium Using VNTR Method

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Noorozi J

2011-06-01

Full Text Available Background and Objectives: Epidemiological studies of Non-tuberculosis Mycobacterium is important because of the drug resistance pattern and worldwide dissemination of these organisms. One of genetic fingerprinting methods for epidemiological studies is VNTR (Variable Number Tandem Repeat. In this study genetic pattern of atypical Mycobacterium was evaluated by VNTR method for epidemiologic studies. Methods: 48 pulmonary and non pulmonary specimens separated from patients with the symptoms of pulmonary tuberculosis (PTB and identified as Non-tuberculosis Mycobacteriumby phenotypic and PCR-RFLP methods were selected for this study. Clinical samples and their standard strains were evaluated according to VNTR pattern using the 7 genetic loci including ETR-B. ETR-F. ETR-C. MPTR-A. ETR-A. ETR-E. ETR-D.Results: The results of VNTR method showed that none of the 7 loci had any polymorphism in the standard strains of atypical mycobacterium. Some of these variable number tandem repeat in 42 clinical samples of non-tuberculosis Mycobacterium were polymorphic while the PCR product (for any loci was not found in the remaining 6 specimens. Conclusion: Although the used genetic loci of this study were suitable for epidemiological studies of Mycobacterium tuberculosis, these loci were not able to determine the diversity of genetics of non-tuberculosis Mycobacterium Therefore, it seems necessary that other loci be studied using VNTR method.

Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis.

Science.gov (United States)

Salazar, L; Fsihi, H; de Rossi, E; Riccardi, G; Rios, C; Cole, S T; Takiff, H E

1996-04-01

The genus Mycobacterium is composed of species with widely differing growth rates ranging from approximately three hours in Mycobacterium smegmatis to two weeks in Mycobacterium leprae. As DNA replication is coupled to cell duplication, it may be regulated by common mechanisms. The chromosomal regions surrounding the origins of DNA replication from M. smegmatis, M. tuberculosis, and M. leprae have been sequenced, and show very few differences. The gene order, rnpA-rpmH-dnaA-dnaN-recF-orf-gyrB-gyrA, is the same as in other Gram-positive organisms. Although the general organization in M. smegmatis is very similar to that of Streptomyces spp., a closely related genus, M. tuberculosis and M. leprae differ as they lack an open reading frame, between dnaN and recF, which is similar to the gnd gene of Escherichia coli. Within the three mycobacterial species, there is extensive sequence conservation in the intergenic regions flanking dnaA, but more variation from the consensus DnaA box sequence was seen than in other bacteria. By means of subcloning experiments, the putative chromosomal origin of replication of M. smegmatis, containing the dnaA-dnaN region, was shown to promote autonomous replication in M. smegmatis, unlike the corresponding regions from M. tuberculosis or M. leprae.

Mycobacterium tuberculosis Infection in a Domesticated Korean Wild Boar ( Sus scrofa coreanus).

Science.gov (United States)

Seo, Min-Goo; Ouh, In-Ohk; Kim, Munki; Lee, Jienny; Kim, Young-Hoan; Do, Jae-Cheul; Kwak, Dongmi

2017-06-01

Tuberculosis, a chronic progressive disease, has been reported in bovine, swine, and primate species. Here, we report the first case of Mycobacterium tuberculosis infection in a Korean wild boar ( Sus scrofa coreanus). The owners this domesticated boar brought it to the Gyeongbuk Veterinary Service Laboratory in Korea after it was found dead and severely emaciated. Demarcated yellowish white nodules were found around the larynx and retropharyngeal lymph node during necropsy. The lungs had diffuse fibrinous pleuritis, severe congestion, and scattered nodules. More nodules were found in the spleen. Tuberculosis is characterized by massive macrophage infiltration and central caseous necrosis; both characteristics were found in the lungs. Histopathologic examination revealed that the alveolar lumen had marked fibrosis and exudates. Examination of the fluid revealed extensive macrophage permeation. To confirm a Mycobacterium infection, PCR was performed using two primer sets specific to the rpoB gene of Mycobacterium; Mycobacterium was detected in the lungs and spleen. To identify the species of Mycobacterium, immunohistochemical evaluation was performed using antibodies against Mycobacterium tuberculosis and Mycobacterium bovis . The results revealed immunoreactivity against M. tuberculosis but not against M. bovis . The consumption of undercooked or raw meat from game animals may expose humans and other animals to sylvatic infection. Consequently, Koreans who ingest wild boar may be at risk of a tuberculosis infection. To reduce the risk of foodborne infection and maintain public health, continuous monitoring and control strategies are required.

Rapid Detection of Cell-Free Mycobacterium tuberculosis DNA in Tuberculous Pleural Effusion.

Science.gov (United States)

Che, Nanying; Yang, Xinting; Liu, Zichen; Li, Kun; Chen, Xiaoyou

2017-05-01

Tuberculous pleurisy is one of the most common types of extrapulmonary tuberculosis, but its diagnosis remains difficult. In this study, we report for the first time on the detection of cell-free Mycobacterium tuberculosis DNA in pleural effusion and an evaluation of a newly developed molecular assay for the detection of cell-free Mycobacterium tuberculosis DNA. A total of 78 patients with pleural effusion, 60 patients with tuberculous pleurisy, and 18 patients with alternative diseases were included in this study. Mycobacterial culture, the Xpert MTB/RIF assay, the adenosine deaminase assay, the T-SPOT.TB assay, and the cell-free Mycobacterium tuberculosis DNA assay were performed on all the pleural effusion samples. The cell-free Mycobacterium tuberculosis DNA assay and adenosine deaminase assay showed significantly higher sensitivities of 75.0% and 68.3%, respectively, than mycobacterial culture and the Xpert MTB/RIF assay, which had sensitivities of 26.7% and 20.0%, respectively ( P pleural effusion showed the highest sensitivity of 95.0% but the lowest specificity of 38.9%. The cell-free Mycobacterium tuberculosis DNA assay detected as few as 1.25 copies of IS 6110 per ml of pleural effusion and showed good accordance of the results between repeated tests ( r = 0.978, P = 2.84 × 10 -10 ). These data suggest that the cell-free Mycobacterium tuberculosis DNA assay is a rapid and accurate molecular test which provides direct evidence of Mycobacterium tuberculosis etiology. Copyright © 2017 American Society for Microbiology.

Anemia hemolítica causada por Indigofera suffruticosa (Leg. Papilionoideae em bovinos Hemolytic anemia caused by Indigofera suffruticosa (Leg. Papilionoideae in cattle

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José Diomedes Barbosa Neto

2001-03-01

Full Text Available As partes aéreas de Indigofera suffruticosa Mill. (família Leg. Papilionoideae, planta incriminada pelos criadores de diversas áreas do Nordeste por doença caracterizada por hemoglobinúria em bovinos, foram administradas por via oral a seis bovinos, em doses diárias repetidas de 10 a 40 g/kg, Todos os animais experimentais apresentaram hemoglobinúria, porém passageira, apesar continuidade da administração da planta. Dois desses bovinos não apresentaram manifestações adicionais, um terceiro animal evidenciou manifestações leves, e os três outros, sintomas adicionais de intensidade moderada: apatia, mucosas visíveis de coloração esbranquiçada, pêlos arrepiados, anorexia, diminuição da freqüência e intensidade dos movimentos ruminais, taquicardia, pulso venoso positivo e dispnéia. Antes da crise hemolítica a urina apresentava coloração verde azulada. Nenhum animal experimental morreu, porém um foi sacrificado durante a fase hemoglobinúrica. À necropsia observaram-se anemia, bexiga contendo urina cor de vinho tinto, rins aumentados de volume com coloração marrom-escura, fígado, na superfície e ao corte, de coloração azulada com lobulação perceptível. As principais alterações histológicas foram verificadas no fígado, sob forma de necrose coagulativa e tumefação e/ou microvacuolização citoplasmática dos hepatócitos, e no rim representadas por acentuada nefrose, associada a grande quantidade de filtrado e/ou hemoglobina nos espaços de Bowman dentro de túbulos e do citoplasma das células epiteliais.The aereal parts of Indigofera suffruticosa Mill. (family Leg. Papilionoideae were force-fed fresh to 6 bovines in daily doses of 10 to 40 g/kg. Cattle breeders of various parts of the Northeast of Brazil accuse this plant as the cause of a non-fatal disease characterized by hemoglobinuria. The disease occurs only in years when the plant proliferates well invading the native pastures. All experimental

Phylogenetic analysis of vitamin B12-related metabolism in Mycobacterium tuberculosis

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Douglas B. Young

2015-03-01

Full Text Available Comparison of genome sequences from clinical isolates of Mycobacterium tuberculosis with phylogenetically-related pathogens Mycobacterium marinum, Mycobacterium kansasii and Mycobacterium leprae reveals diversity amongst genes associated with vitamin B12-related metabolism. Diversity is generated by gene deletion events, differential acquisition of genes by horizontal transfer, and single nucleotide polymorphisms with predicted impact on protein function and transcriptional regulation. Differences in the B12 synthesis pathway, methionine biosynthesis, fatty acid catabolism, and DNA repair and replication are consistent with adaptations to different environmental niches and pathogenic lifestyles. While there is no evidence of further gene acquisition during expansion of the M. tuberculosis complex, the emergence of other forms of genetic diversity provides insights into continuing host-pathogen co-evolution and has the potential to identify novel targets for disease intervention.

Adolescente con inestabilidad lumbosacra por presencia de quiste hidatídico: reporte de caso

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Miguel Vizcarra

Full Text Available La hidatidosis, causada por el Echinococcus granulosus, afecta a la columna vertebral en 0,5-2% del total de casos, siendo la columna sacra una ubicación rara, típica de personas en edad adulta. Se reporta el caso de un varón de 14 años oriundo de Juliaca (Puno, Perú quien presentó lumbalgia crónica y síndrome de cauda equina. Atendido inicialmente en Juliaca, fue trasladado al Hospital Edgardo Rebagliati Martins (Lima, Perú con el diagnóstico de tumoración sacra. En la resonancia magnética se mostró una tumoración multiquística con lisis del L5 y S1 e inestabilidad. El tratamiento quirúrgico fue una laminectomía L5-S2 y extracción de múltiples quistes de hidátide más estabilización lumbopélvica con tornillos transpediculares. En el posoperatorio se recetó albendazol (15 mg/kg/d por el periodo de seis meses. Se debe considerar al quiste hidatídico dentro del diagnóstico diferencial cuando existan lesiones quísticas en la columna vertebral, sobre todo en regiones endémicas como el Perú.

Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis

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Helene Botella

2017-09-01

Full Text Available Peptidoglycan (PG, a polymer cross-linked by d-amino acid-containing peptides, is an essential component of the bacterial cell wall. We found that a fluorescent d-alanine analog (FDAA incorporates chiefly at one of the two poles in Mycobacterium smegmatis but that polar dominance varies as a function of the cell cycle in Mycobacterium tuberculosis: immediately after cytokinesis, FDAAs are incorporated chiefly at one of the two poles, but just before cytokinesis, FDAAs are incorporated comparably at both. These observations suggest that mycobacterial PG-synthesizing enzymes are localized in functional compartments at the poles and septum and that the capacity for PG synthesis matures at the new pole in M. tuberculosis. Deeper knowledge of the biology of mycobacterial PG synthesis may help in discovering drugs that disable previously unappreciated steps in the process.

Rotavirus morbidity and mortality in children in Brazil Morbilidad y mortalidad por rotavirus en niños en Brasil

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Ana Marli Christovam Sartori

2008-02-01

Full Text Available OBJECTIVE: To study the epidemiology of rotavirus and estimate rotavirus-associated morbidity and mortality in children OBJETIVOS: Analizar la epidemiología del rotavirus y estimar la morbilidad y la mortalidad asociadas con las infecciones por rotavirus en niños < 5 años de edad en Brasil en 2004, antes de incluir la vacuna contra el rotavirus en el Programa Nacional de Inmunizaciones (PNI. MÉTODOS: Para estimar la morbilidad por rotavirus se revisaron los estudios publicados (1999-2006 que abordaban la incidencia de diarrea aguda en niños < 5 años de edad y la frecuencia de las infecciones por rotavirus en niños con diarrea en Brasil. Los casos de diarrea se dividieron en tres categorías de gravedad según el nivel de atención que requirieron: casos leves que solo requirieron atención domiciliaria, casos moderados que requirieron la visita a un servicio ambulatorio de salud y casos graves que requirieron hospitalización. Para estimar la mortalidad por rotavirus se utilizó el número de muertes registradas por diarrea en niños de < 5 años, según el Sistema de Información sobre Mortalidad (SIM del Sistema Único de Salud (SUS de Brasil, y se calculó la proporción de muertes causadas por este virus. RESULTADOS: Se estimó que las infecciones por rotavirus causan anualmente 3 525 053 casos de diarrea, 655 853 visitas a servicios ambulatorios de salud, 92 453 hospitalizaciones y 850 muertes en niños < 5 años de edad en Brasil. CONCLUSIONES: Las infecciones por rotavirus constituyen una importante causa de morbilidad y mortalidad en Brasil.

Desenvolvimento de metodologia para determinação de multirresiduos de herbicidas e seus metabolitos em agua e em solo por cromatografia liquida de alta eficiencia

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Glaucia Maria Ferreira Pinto

2002-01-01

Resumo: Com o crescimento da população mundial, a preocupação com o aumento da produção agrícola torna-se mais pronunciada. Para garantir um maior rendimento agrícola tem-se intensificado o uso de agrotóxicos, o que aumentou a contaminação ambiental causada por estes compostos químicos. No presente trabalho foram desenvolvidos e validados métodos envolvendo o uso de extração em fase sólida e cromatografia líquida de alta eficiência, aplicados à análise de amostras ambientais de águas e solos,...

Desarrollo de panuveítis por tuberculosis en paciente con esclerosis múltiple tratado con interferón beta

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Lucía Echevarría Lucas

2016-09-01

Los linfocitos CD4+ T, leucocitos, macrófagos y granulocitos, con la producción de sus mediadores interferón gamma, IL-12 o TNF-α son fundamentales para controlar al Mycobacterium tuberculosis. Por ello, antes de introducir Interferón beta 1b, convendría realizar técnicas de screening, como la prueba de Mantoux o el interferon gamma release assay–(quantiferon-TB para detectar posibles tuberculosis latentes potencialmente activables.

Infections with Mycobacterium tuberculosis and Mycobacterium avium among HIV-infected patients after the introduction of highly active antiretroviral therapy. EuroSIDA Study Group JD

DEFF Research Database (Denmark)

Kirk, O; Gatell, J M; Mocroft, A

2000-01-01

the introduction of HAART, using data from the EuroSIDA study, a European, multicenter observational cohort of more than 7,000 patients. Overall incidences of Mycobacterium tuberculosis (TB) and Mycobacterium avium complex (MAC) were 0.8 and 1.4 cases/100 person-years of follow-up (PYF), decreasing from 1.8 (TB...

Comportamento tintorial do Mycobacterium leprae: revisão histórica Tinctorial behavior of Mycobacterium leprae: a historical review

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Luiz Fernando de Góes Siqueira

1983-08-01

Full Text Available Foi feita revisão histórica sobre os corantes utilizados na identificação do Mycobacterium leprae. Foram analisadas para cada corante, sua composição química, propriedades tintoriais e a capacidade de assimilação pelo bacilo nas diversas técnicas de coloração.A historical review was made of the dyes utilized to identify the Mycobacterium leprae. The chemical composition and the tinctorial properties of these substances and the dye assimilation capacity of the bacilli were analyzed.

Risk factors for Mycobacterium tuberculosis infection among children in Greenland

DEFF Research Database (Denmark)

Søborg, Bolette; Andersen, Aase Bengaard; Melbye, Mads

2011-01-01

To examine the risk factors for Mycobacterium tuberculosis infection (MTI) among Greenlandic children for the purpose of identifying those at highest risk of infection.......To examine the risk factors for Mycobacterium tuberculosis infection (MTI) among Greenlandic children for the purpose of identifying those at highest risk of infection....

Treinta días persiguiendo la muerte: miseria, dolor y obras de caridad en Bogotá de 1918, desolada por la epidemia de gripa

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Álvaro Miranda

2008-09-01

Full Text Available Para finales de la segunda década del siglo xx, Bogotá tiene poco que mostrar. Está detenida en el tiempo, sumergida en una rutina en la que las lluvias casi siempre le ganan a los días de sol y el barro salpica todo, tanto a la gente elegante como a la gente sencilla, que visten lo mejor de sus prendas para presentarse en calles, almacenes y templos. La rutina menuda de hombres y mujeres y la rutina trascendente de políticos y escritores en diarios y revistas, fue sustituida, de repente, por la de la muerte. Nada similar para comparar tenían en su memoria esos bogotanos que en los meses de octubre y noviembre de 1918 tuvieron que vivir en su ciudad una pesadilla causada por una gripa que de igual modo hacía estragos en muchos otros lugares del mundo.

Pneumonia por varicela associada com síndrome da angústia respiratória aguda: relato de dois casos Varicella pneumonia complicated with acute respiratory distress syndrome: two cases report

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Marcelo Moreno; Ricardo Castelão; Susana Orrico Peres; Suzana Margareth Lobo

2007-01-01

JUSTIFICATIVA E OBJETIVOS: A varicela é uma doença exantemática causada pela infecção primária do vírus varicela zoster (VVZ). A pneumonia pelo VVZ complicada com a síndrome da angústia respiratória aguda (SARA) é rara e associa-se a altas taxas de morbimortalidade. O objetivo deste estudo foi apresentar dois casos de pneumonia por varicela que evoluíram com SARA e outras disfunções orgânicas. RELATO DOS CASOS: Paciente de 15 anos, imunocomprometido com a síndrome da imunodeficiência adquirid...

SUSCEPTIBILITY OF RIFAMPICIN-ISONIAZID RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES AGAINST LEVOFLOXACIN

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A. H. Kurniawan

2016-01-01

Full Text Available Background: Tuberculosis (TB is a high burden disease in Indonesia with multidrug-resistant (MDR TB incidence started to increase. Treatment success of MDR-TB globally was low in number than it was targeted which was especially caused by fluoroquinolone resistance. One of the fluoroquinolone is levofloxacin, an antibiotic that has been widely used irrationally as antimicrobial treatment. Therefore, this study investigated the sensitivity and MBC of MDR Mycobacterium tuberculosis isolates against Levofloxacin. Method: The susceptibility test for MDR-Mycobacterium tuberculosis on levofloxacin by standard method with levofloxacin were on concentrations 0,5 μg/ml, 1 μg/ml, and 2 μg/ml. Sample of 8 strains MDR-Mycobacterium tuberculosis were cultured with each concentrations on Middlebrook 7H9 for 1 week incubation. Next, each of the incubated concentration was subcultured on solid media Middlebrook 7H10 for 3 weeks incubation. Colonized agar plates after 3 weeks incubation were confirmed with acid-fast stain. Results: On MB 7H10 with levofloxacin concentration 2 μg/ml showed bactericidal effect 100% by no MDR Mycobacterium tuberculosis colony grew (0/8 while the MB 7H10 with levofloxacin concentration 1 μg/ml and 0,5 μg/ml showed the bactericidal effect 37,5% and 25% respectively. The colonized agar plate implied that the MDR Mycobacterium tuberculosis with levofloxacin concentration 1 μg/ml (5/8 and 0,5 μg/ml (6/8 grew well. Conclusion: Levofloxacin concentration 2 μg/ml was susceptible on MDR Mycobacterium tuberculosis. The concentration 2 μg/ml of levofloxacin could be considered as MBC.

Lesões craniofaciais decorrentes de acidentes por motocicleta: uma revisão integrativa

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Maria Gabriella Pacheco da Silva

2015-10-01

Full Text Available Resumo: Os acidentes de trânsito têm constituído um problema de saúde pública que mais cresce no Brasil e no mundo, com destaque para as motocicletas. Além dos altos índices de mortalidade, destacam-se também as lesões e sequelas causadas aos sobreviventes ao acidente. As lesões de face são comuns neste tipo de acidente, causando deformidades estéticas e funcionais no rosto, necessitando de reabilitação. Foi realizada uma busca na literatura para mapear as lesões na região craniofacial decorrentes de acidentes de trânsito por motocicleta, buscando também suas possíveis implicações para a Comunicação Humana. As bases de dados Pubmed, Lilacs e Scielo foram acessadas, selecionando os artigos pelos títulos, em seguida pelos resumos, para ao final selecionar os artigos para leitura na íntegra. Após este processo, foram selecionados 10 estudos para a revisão. Na região facial, as fraturas mais frequentes foram nos ossos da Mandíbula, Zigomático e Nasal, por serem os mais proeminentes na região. Destacaram-se os Traumas Cranianos como os tipos de lesões com maior gravidade, uma vez que podem levar ao óbito. Apesar do detalhamento das lesões, as sequelas e dados sobre a reabilitação dessas vítimas de acidentes por motocicletas foram raros na literatura.

Tratamiento de la enfermedad de Hansen en Colombia: medicalización y control de la enfermedad a lo largo del siglo XX

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Natalia Botero-Jaramillo

2017-09-01

Full Text Available Objetivo: investigar el tratamiento de la enfermedad de Hansen durante el siglo XX en Colombia, para analizar el proceso de medicalización, la biopolítica, el significado de la curación y su impacto en el control de la enfermedad y en los enfermos. Metodología: estudio histórico, descriptivo y analítico sobre los tratamientos para la enfermedad de Hansen a lo largo del siglo XX, por medio de metodologías cualitativas como entrevistas con relatos de vida y entrevistas temáticas, y se trabajó con fuentes documentales primarias y secundarias en archivos nacionales y locales. Resultados y discusión: La enfermedad de Hansen, causada por el microorganismo Mycobacterium leprae, fue controlada mediante el aislamiento y reclusión de los enfermos de lepra en hospitales y lazaretos. El desarrollo de algunos tratamientos y el descubrimiento de la terapia antibiótica, las sulfas, que representó una cura efectiva desde la medicina y su poder político y social, participaron en la transformación de las políticas de manejo de la enfermedad y de la comprensión de la misma, en los Lazaretos de Contratación y Agua de Dios.

Pott's disease: a case of Mycobacterium xenopi infection of the spine.

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Alfreijat, Majd; Ononiwu, Chiagozie; Sexton, Carlton

2012-01-01

Pott's disease is an infection of the spine with Mycobacterium tuberculosis that causes destruction of the spine elements resulting in progressive kyphosis. We are describing a rare case of Pott's disease where Mycobacterium xenopi was the inculpated organism.

Mycobacterium eburneum sp. nov., a non-chromogenic, fast-growing strain isolated from sputum.

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Nouioui, Imen; Carro, Lorena; Teramoto, Kanae; Igual, José M; Jando, Marlen; Del Carmen Montero-Calasanz, Maria; Sutcliffe, Iain; Sangal, Vartul; Goodfellow, Michael; Klenk, Hans-Peter

2017-09-01

A polyphasic study was undertaken to establish the taxonomic position of a non-chromogenic, rapidly growing Mycobacterium strain that had been isolated from sputum. The strain, CECT 8775T, has chemotaxonomic and cultural properties consistent with its classification in the genus Mycobacterium and was distinguished from the type strains of closely related mycobacterial species, notably from Mycobacterium paraense DSM 46749T, its nearest phylogenetic neighbour, based on 16S rRNA, hsp65 and rpoB gene sequence data. These organisms were also distinguished by a broad range of chemotaxonomic and phenotypic features and by a digital DNA-DNA relatedness value of 22.8 %. Consequently, the strain is considered to represent a novel species of Mycobacterium for which the name Mycobacterium eburneum sp. nov is proposed; the type strain is X82T (CECT 8775T=DSM 44358T).

Miocardiopatia hipertrófica não sarcomérica : casos clínicos

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Azevedo, Pedro Miguel Cacais Rua

2017-01-01

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2017 A miocardiopatia hipertrófica é uma patologia em que há hipertrofia miocárdica não causada por patologia hipertensiva, valvular ou outras situações que decorrem com aumento de pós-carga. A miocardiopatia hipertrófica não sarcomérica, tratada neste trabalho, não é causada por mutações em proteínas do sarcómero. Uma das causas da miocardiopatia hipertrófica é a doença de Fabry, d...

Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169

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We report the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169T, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, though Fl-016...

Análise genotípica de isolados de Mycobacterium tuberculosis de um hospital em Lisboa, Portugal

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João Perdigão

2009-09-01

Full Text Available Resumo: Portugal apresenta uma das taxas de incidência de tuberculose mais altas da União Europeia, tendo a Região de Saúde de Lisboa uma taxa de incidência muito acima da média nacional. O presente estudo analisa a transmissão, susceptibilidade aos antibacilares e características de uma população de estudo de um hospital central de Lisboa. Cento e trinta e dois isolados clínicos de Mycobacterium tuberculosis foram pre-viamente testados quanto à susceptibilidade aos antibacilares de primeira linha. A taxa de multirresistência encontrada foi de 3,0%, enquanto 13,6% dos isolados eram resistentes a um ou mais antibacilares de primeira linha. A serologia para o VIH estava disponível para 98 doentes, 26 (26,5% eram positivos. Os isolados foram genotipados por MIRU-VNTR e 53 (40,2% dos 132 isolados encontravam-se distribuídos por 17 clusters MIRU-VNTR diferentes de dois ou mais isolados. De todos os isolados analisados, 25,8% perten-ciam à família Lisboa. Concluímos que a transmissão de estirpes de Mycobacterium tuberculosis, resistentes e susceptíveis, está a ocorrer, com especial preocupação para as estirpes Lisboa.Rev Port Pneumol 2009; XV (5: 761-769 Abstract: Portugal has one of the highest tuberculosis notification rates of the European Union with Lisbon Health Region having an incidence rate well above the national average. The present study analyses the transmission, drug susceptibility and characteristics of a study population from a Central Lisbon’s Hospital. One hundred and thirty-two Mycobacterium tuberculosis clinical isolates were previously tested for drug susceptibility to first-line drugs. The multidrug (MDR resistance rate was found to be 3.0%, while 13.6% of the isolates were resistant to one or more first-line drugs. HIV serology was available for 98 patients, 26 (26.5% were positive. Genotyping was performed by MIRU-VNTR and 53 (40,2% out of the 132 isolates

Pott's disease: a case of Mycobacterium xenopi infection of the spine

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Majd Alfreijat

2013-01-01

Full Text Available Pott's disease is an infection of the spine with Mycobacterium tuberculosis that causes destruction of the spine elements resulting in progressive kyphosis. We are describing a rare case of Pott's disease where Mycobacterium xenopi was the inculpated organism.

Enfermedad pulmonar obstructiva crónica por exposición al humo de biomasa

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Matías Lopez

Full Text Available En este artículo se discute la relación existente entre la enfermedad pulmonar obstructiva crónica (EPOC y el humo de biomasa. Más de la mitad de la población utiliza biomasa como combustible principal, sobre todo en áreas rurales y en países en vías de desarrollo donde su uso llega hasta el 80%. La inhalación del humo de biomasa crea un estado inflamatorio crónico, que se acompaña de una activación de metaloproteinasas y una reducción de la movilidad mucociliar. Esto podría explicar la gran asociación existente entre la exposición a biomasa y EPOC, revelada por estudios observacionales y epidemiológicos provenientes de países en vías de desarrollo y de países desarrollados. En esta revisión exploramos también las diferencias entre la EPOC causada por tabaco y por biomasa, y encontramos que, a pesar de las diferencias fisiopatológicas, la mayoría de las características clínicas, calidad de vida y mortalidad fueron parecidas. En los últimos diez años se han realizado intervenciones para disminuir la exposición a biomasa mediante el uso de cocinas mejoradas y combustibles limpios, sin embargo, estas estrategias todavía no han sido exitosas debido a su incapacidad para reducir los niveles de contaminación a niveles recomendados por la Organización Mundial de la Salud, y por su falta de uso. Por lo tanto, hay una necesidad urgente de ensayos de campo aleatorios, cuidadosamente realizados, para determinar la verdadera gama de reducciones de contaminación potencialmente alcanzables, la probabilidad de su uso y los beneficios a largo plazo en la reducción de la gran carga mundial de EPOC

Vertebral Osteomyelitis Caused by Mycobacterium abscessus Surgically Treated Using Antibacterial Iodine-Supported Instrumentation

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Satoshi Kato

2014-01-01

Full Text Available Mycobacterium abscessus infections rarely develop in healthy individuals, and mostly they occur in immunocompromised hosts. Vertebral osteomyelitis due to Mycobacterium abscessus is very rare and only three previous cases of spinal infection caused by Mycobacterium abscessus have been reported. Mycobacterium abscessus isolates are uniformly resistant to antituberculous agents and can display a virulent biofilm-forming phenotype. The patient was a 67-year-old woman with vertebral osteomyelitis of the L1-2. She was healthy without immune-suppressed condition, history of trauma, or intravenous drug use. The smear examination of the specimen harvested by CT-guided puncture of the paravertebral abscess revealed Mycobacterium abscessus. Her disease condition did not abate with conservative treatment using antimicrobial chemotherapy. Radical debridement of the vertebral osteomyelitis and anterior reconstruction from T12 to L2 using antibacterial iodine-supported instrumentation were performed. Chemotherapy using clarithromycin, amikacin, and imipenem was applied for 6 months after surgery as these antibiotics had been proven to be effective to Mycobacterium abscessus after surgery. Two years after surgery, the infected anterior site healed and bony fusion was successfully achieved without a recurrence of infection.

Disseminated Infection by Mycobacterium sherrisii and Histoplasma capsulatum in an African HIV-Infected Patient

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Taján, Juan; Espasa, Mateu; Sala, Montserrat; Navarro, Marta; Font, Bernat; González-Martín, Julián; Segura, Ferran

2013-01-01

Mycobacterium sherrisii is a new species of opportunistic, slow-growing, non-tuberculous Mycobacterium closely related to Mycobacterium simiae that can currently be identified with the sequence of 16S rARN gene and the heat-shock protein 65. Few cases of patients infected by this Mycobacterium have been reported and all of them were associated with human immunodeficiency virus or other immunosuppressive conditions. Clinical management is complex, because there is not a clear correlation between the in vitro antibiotic susceptibility testing and the patient's clinical outcome. PMID:23419367

Methylobacterium spp. as an indicator for the presence or absence of Mycobacterium spp.

OpenAIRE

Falkinham III, Joseph O.; Williams, Myra D.; Kwait, Rebecca; Lande, Leah

2016-01-01

Objective/Background: A published survey of bacteria in showerhead biofilm samples revealed that Methylobacterium spp. and Mycobacterium spp. seldom coexisted in biofilms. Method: To confirm that information, biofilm samples were collected from household plumbing of Mycobacterium avium patients and Methylobacterium spp. and M. avium numbers were measured by direct colony counts. Results: The results demonstrated that if Methylobacterium spp. were present, Mycobacterium spp. were absent,...

Cervical Lymphadenitis by Mycobacterium triplex in an Immunocompetent Child: Case Report and Review

OpenAIRE

Caruso, G.; Angotti, R.; Molinaro, F.; Benicchi, E.; Cerchia, E.; Messina, M.

2013-01-01

Mycobacterium triplex was first described in 1996. This nontuberculous Mycobacterium causes a severe pulmonary disease in immunocompromised patients but it can involve also healthy patients. A literature search was made on the PubMed database and it produced only few cases of children with cervical lymphadenitis due to this Mycobacterium Triplex. We are describing a case of M. triplex cervical lymphadenitis in an immunocompetent child.

MYCOBACTERIUM GENAVENSE IN AN AFRICAN PENGUIN (SPHENISCUS DEMERSUS).

Science.gov (United States)

Krause, Kristian J; Reavill, Drury; Weldy, Scott H; Bradway, Daniel S

2015-12-01

A 19-yr-old female African penguin (Spheniscus demersus) presented with labored breathing and anorexia. Radiographs revealed soft-tissue density lesions in the left lung fields and fluid in the right. The penguin died during the night. Postmortem examination demonstrated multiple granulomas in the lungs and air sacs. The right coelom was filled with opaque fluid. Histopathology of the lung, liver, kidney, and spleen identified Mycobacterium as a primary disease etiology. Large numbers of acid fast-positive, rod-shaped bacteria were recognized on tissue staining. Mycobacterium genavense was detected by polymerase chain reaction (PCR) using primers specific for the species. Further confirmation of M. genavense was accomplished using PCR with universal Mycobacterium spp. primers followed by sequencing of the amplicon obtained. To our knowledge, this is the first reported case of mycobacteriosis-and specifically M. genavense -in an African penguin. This case also demonstrates the similarities of presentation between the more commonly suspected and encountered aspergillosis and mycobacteriosis.

Gravidade das coinfecções virais em lactentes hospitalizados com infecção por vírus sincicial respiratório

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Milena De Paulis

2011-08-01

Full Text Available OBJETIVO: Comparar a gravidade de infecções causadas por um único vírus (VSR com a gravidade de coinfecções. MÉTODOS: Este estudo avaliou uma coorte histórica de lactentes com infecção aguda por VSR. Secreção de nasofaringe foi coletada de todos os pacientes rotineiramente para pesquisa viral usando técnicas de biologia molecular. Os seguintes desfechos foram analisados: tempo total de internação, duração da oxigenioterapia, admissão em unidade de terapia intensiva e uso de ventilação mecânica. Os resultados foram ajustados para os fatores confundidores (prematuridade, idade e aleitamento materno. RESULTADOS: Foram incluídos no estudo 176 lactentes com idade média de 4,5 meses e diagnósticos de bronquiolite e/ou pneumonia. Cento e vinte e um tinham infecção única por VSR, e 55 tinham coinfecções (24 VSR + adenovírus, 16 VSR + metapneumovírus humano e 15 outras associações menos frequentes. Os quatro desfechos de gravidade avaliados foram semelhantes entre o grupo com infecção única por VSR e os grupos com coinfecções, independente do tipo de vírus associado com o VSR. CONCLUSÃO: As coinfecções virais não parecem alterar o prognóstico de lactentes hospitalizados com infecção aguda por VSR.

Imaging features of mycobacterium in patients with acquired immunodeficiency syndrome

International Nuclear Information System (INIS)

Yang Jun; Sun Yue; Wei Liangui; Xu Yunliang; Li Xingwang

2013-01-01

Objective: To analyze the imaging features of mycobacterium in AIDS patients. Methods: Twenty-three cases of mycobacterium tuberculosis and 13 patients of non-tuberculous mycobacteria were proved etiologically and included in this study. All patients underwent X-ray and CT examinations, imaging data were analyzed and compared. Results: The imaging findings of mycobacterium tuberculosis in AIDS patients included consolidation (n = 11), pleural effusion (n = 11), mediastinal lymphadenopathy (n = 11). Pulmonary lesions were always diffuse distribution, and 14 patients of extrapulmonary tuberculosis were found. Pulmonary lesions in non-tuberculous mycobacteria tend to be circumscribed. Conclusions: Non-tuberculous mycobacterial infection in AIDS patients is more common and usually combined with other infections. Imaging features are atypical. (authors)

Mycobacterium Diversity and Pyrene Mineralization in Petroleum-Contaminated Soils

OpenAIRE

Cheung, Pui-Yi; Kinkle, Brian K.

2001-01-01

Degradative strains of fast-growing Mycobacterium spp. are commonly isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soils. Little is known, however, about the ecology and diversity of indigenous populations of these fast-growing mycobacteria in contaminated environments. In the present study 16S rRNA genes were PCR amplified using Mycobacterium-specific primers and separated by temperature gradient gel electrophoresis (TGGE), and prominent bands were sequenced to compare the ...

A novel multi-antigen virally vectored vaccine against Mycobacterium avium subspecies paratuberculosis.

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Tim J Bull

Full Text Available BACKGROUND: Mycobacterium avium subspecies paratuberculosis causes systemic infection and chronic intestinal inflammation in many species including primates. Humans are exposed through milk and from sources of environmental contamination. Hitherto, the only vaccines available against Mycobacterium avium subspecies paratuberculosis have been limited to veterinary use and comprised attenuated or killed organisms. METHODS: We developed a vaccine comprising a fusion construct designated HAV, containing components of two secreted and two cell surface Mycobacterium avium subspecies paratuberculosis proteins. HAV was transformed into DNA, human Adenovirus 5 (Ad5 and Modified Vaccinia Ankara (MVA delivery vectors. Full length expression of the predicted 95 kDa fusion protein was confirmed. PRINCIPAL FINDINGS: Vaccination of naïve and Mycobacterium avium subspecies paratuberculosis infected C57BL/6 mice using DNA-prime/MVA-boost or Ad5-prime/MVA-boost protocols was highly immunogenic resulting in significant IFN-gamma ELISPOT responses by splenocytes against recombinant vaccine antigens and a range of HAV specific peptides. This included strong recognition of a T-cell epitope GFAEINPIA located near the C-terminus of the fusion protein. Antibody responses to recombinant vaccine antigens and HAV specific peptides but not GFAEINPIA, also occurred. No immune recognition of vaccine antigens occurred in any sham vaccinated Mycobacterium avium subspecies paratuberculosis infected mice. Vaccination using either protocol significantly attenuated pre-existing Mycobacterium avium subspecies paratuberculosis infection measured by qPCR in spleen and liver and the Ad5-prime/MVA-boost protocol also conferred some protection against subsequent challenge. No adverse effects of vaccination occurred in any of the mice. CONCLUSIONS/SIGNIFICANCE: A range of modern veterinary and clinical vaccines for the treatment and prevention of disease caused by Mycobacterium avium

A novel multi-antigen virally vectored vaccine against Mycobacterium avium subspecies paratuberculosis.

Science.gov (United States)

Bull, Tim J; Gilbert, Sarah C; Sridhar, Saranya; Linedale, Richard; Dierkes, Nicola; Sidi-Boumedine, Karim; Hermon-Taylor, John

2007-11-28

Mycobacterium avium subspecies paratuberculosis causes systemic infection and chronic intestinal inflammation in many species including primates. Humans are exposed through milk and from sources of environmental contamination. Hitherto, the only vaccines available against Mycobacterium avium subspecies paratuberculosis have been limited to veterinary use and comprised attenuated or killed organisms. We developed a vaccine comprising a fusion construct designated HAV, containing components of two secreted and two cell surface Mycobacterium avium subspecies paratuberculosis proteins. HAV was transformed into DNA, human Adenovirus 5 (Ad5) and Modified Vaccinia Ankara (MVA) delivery vectors. Full length expression of the predicted 95 kDa fusion protein was confirmed. Vaccination of naïve and Mycobacterium avium subspecies paratuberculosis infected C57BL/6 mice using DNA-prime/MVA-boost or Ad5-prime/MVA-boost protocols was highly immunogenic resulting in significant IFN-gamma ELISPOT responses by splenocytes against recombinant vaccine antigens and a range of HAV specific peptides. This included strong recognition of a T-cell epitope GFAEINPIA located near the C-terminus of the fusion protein. Antibody responses to recombinant vaccine antigens and HAV specific peptides but not GFAEINPIA, also occurred. No immune recognition of vaccine antigens occurred in any sham vaccinated Mycobacterium avium subspecies paratuberculosis infected mice. Vaccination using either protocol significantly attenuated pre-existing Mycobacterium avium subspecies paratuberculosis infection measured by qPCR in spleen and liver and the Ad5-prime/MVA-boost protocol also conferred some protection against subsequent challenge. No adverse effects of vaccination occurred in any of the mice. A range of modern veterinary and clinical vaccines for the treatment and prevention of disease caused by Mycobacterium avium subspecies paratuberculosis are needed. The present vaccine proved to be highly

Sporotrichoid-Like Spread of Cutaneous Mycobacterium chelonae in an Immunocompromised Patient

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Daria Marley Kemp

2017-01-01

Full Text Available Mycobacterium chelonae is a rapidly growing mycobacterium found in water and soil that can cause local cutaneous infections in immunocompetent hosts but more frequently affects immunocompromised patients. Typically, patients will present with painful subcutaneous nodules of the joints or soft tissues from traumatic inoculation. However, exhibiting a sporotrichoid-like pattern of these nodules is uncommon. Herein, we report a case of sporotrichoid-like distribution of cutaneous Mycobacterium chelonae in a patient with systemic lupus erythematosus on significant immunosuppressive medications. Clinicians treating immunocompromised patients should be cognizant of their propensity to develop unusual infections and atypical presentations.

Infecção natural por Corynespora cassiicola em acessos de mamoeiro

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Marcela Tonini Venturini

2014-09-01

Full Text Available Corynespora cassiicola, um patógeno com ampla gama de hospedeiros, tem causado graves danos nos viveiros de mamoeiro na Bahia. Avaliou-se, em ambiente misto de cobertura e a pleno sol, a incidência natural de C. cassiicola em 49 acessos de mamoeiro em experimento conduzido em blocos casualizados com quatro repetições, 49 tratamentos e variável número de plantas/tratamento. O número de plantas com sintomas e mortas por C. cassiicola foi avaliado aos 27 e 32 dias após a semeadura. A análise de Cluster (p< 0,05 classificou os acessos em 5 grupos pelo número médio de plântulas infectadas e mortas. Os acessos com menor porcentagem de plântulas infectadas foram: Sunrise solo 72/12, Sunrise solo, Golden comercial, Grampola, Kapoho solo (polpa vermelha, Mamão roxo e Santa Helena (50A PLT - 09, 14A PLT - 05, 12A PLT- 06, 12A PLT - 07, 02A PLT -01. Nova sintomatologia causada pelo patógeno é descrita.

Radiometric assessment of the sensitivity to antituberculotics of Mycobacterium avium-intracellulare and Mycobacterium xenopi

International Nuclear Information System (INIS)

Kubin, M.; Lindholm-Levy, P.; Heifets, L. B.

1994-01-01

The macrodilution radiometric method using Middlebrook's 7H12 liquid medium enriched with 14 C-palmitic acid, where the growth activity is monitored by measuring liberated 14 CO 2 , was applied to 25 strains of the Mycobacterium avium complex and to 20 strains of Mycobacterium xenopi to determine the minimal inhibitory concentrations of the following chemotherapeutical agents: ciprofloxacine, clofazimine, rifampin, cycloserine, kanamycin, etionamide, ethambutol, and amikacin. In the case of the M. avium complex, slightly or completely resistant strains were found for the majority of drugs. The sensitive strain proportion was highest with clofazimine and amikacin. The M. xenopis strains exhibited generally lower minimal inhibitory concentrations than the avian mycobacteria for all drugs except for cycloserine and ethambutol. The radiometric method using the BACTEC system was found suitable for the determination of the sensitivity of mycobacteria to chemotherapeutic agents: the results are obtained rapidly, within 8 days following inoculation, and the minimal inhibitory concentrations can be evaluated quantitatively. 1 tab., 8 refs

Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis

NARCIS (Netherlands)

Geluk, A.; van Meijgaarden, K. E.; Franken, K. L. M. C.; Wieles, B.; Arend, S. M.; Faber, W. R.; Naafs, B.; Ottenhoff, T. H. M.

2004-01-01

Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all

Papiloma vírico

OpenAIRE

Albiol Ferrer, Josep Maria; Giralt de Veciana, Enrique; Novel Martí, Virginia; Ogalla, José Manuel; Zalacain, Antonio

1990-01-01

Tumoración benigna y circunscrita de la piel; causada por el 'Papova virus'. constituido por tejido fibroso conjuntivo de sostén que contiene a las papilas y rodeadas de tejido epitelial que lo encapsula. (anida en el tejido papilar)

Energía Azul: generación de potencia a través de la Ósmosis por Presión Retardada (PRO

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Carlos Esteban Aristizabal Alzate

2018-02-01

Full Text Available La emisión de gases efecto invernadero, causadas por el uso de combustibles fósiles y el crecimiento exponencial de la humanidad, vuelca los esfuerzos hacia la búsqueda de fuentes de energía limpia y sostenible, que satisfagan las necesidades energéticas actuales y futuras. Por lo que es imperativo, investigar y desarrollar tecnologías que aprovechen las energías renovables, una de ellas es la Ósmosis por Presión Retardada (PRO, proceso que aprovecha el gradiente de salinidad entre dos soluciones para generar electricidad, ya que la mezcla de una solución salina de concentración alta con agua fresca disipa más de 2,2 MJ/m3 de agua dulce tratada. A escala industrial, se podría aprovechar la diferencia de salinidad entre ríos y mares, cerca de los estuarios. La aplicación, viabilidad y eficiencia del proceso y la tecnología PRO, dependen de las características de la membrana semipermeable, como la potencia por unidad de área, y las propiedades fisicoquímicas y de superficie de esta. En la actualidad, se utilizan membranas de acetato de celulosa, las cuales dan potencias (1W/m2. Sin embargo, se está investigando con membranas de materiales compuestos, como, por ejemplo, las basadas en grafeno, las cuales permiten un mayor flujo de agua y potencias superiores a 5 W/m2.

Transcripción de genes relacionados con la biosíntesis de sulfolípido y poliaciltrehalosas en mycobacterium tuberculosis y detección de su estado metabólico bajo condiciones experimentales de latencia

OpenAIRE

Rodríguez Murillo , Jimmy Esneider

2012-01-01

Mycobacterium tuberculosis (Mtb) es el patógeno de origen bacteriano que causa el mayor número de muertes en el mundo. Según reportes de la Organización Mundial de la Salud (OMS), aproximadamente un tercio de la población mundial, se encuentra infectada con Mtb, una situación que se complica por la multirresistencia a los compuestos antituberculosos. Mtb puede sobrevivir en los individuos infectados por décadas sin causar manifestaciones clínicas en un estado conocido como tuberculosis (TB) l...

Evolución de la mortalidad por atropellos y colisiones de tránsito en Argentina entre 2001 y 2010

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Gabriel Escanés

2015-04-01

Full Text Available Resumen En este artículo se analiza la mortalidad vial con el objetivo de medir el impacto que tuvieron los decesos por atropellos y colisiones de tránsito sobre la esperanza de vida de los argentinos entre los trienios 2000-2002 y 2009-2011. Se aplicó el índice de años de esperanza de vida perdidos (AEVP, que sirvió para establecer la cantidad de años que deberían haber vivido las personas que fallecieron por lesiones causadas al transitar por la vía pública, ya sea como peatones, ciclistas, motociclistas u ocupantes de vehículos a motor. Los registros sobre la población segmentada por edad y sexo se tomaron de los dos últimos censos nacionales de población, correspondientes a los años 2001 y 2010. La información sobre las causas de muerte, sexo y edad de los fallecidos se obtuvo de las bases de datos de la Dirección de Estadísticas e Investigación en Salud. Los principales resultados indican que en el trienio 2000-2002 la población argentina perdió 0,59 años (siete meses de esperanza de vida entre el nacimiento y los 80 años como consecuencia de las muertes en el espacio vial, y 0,66 años (ocho meses en el trienio 2009-2011.

Mycobacterium stephanolepidis sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Stephanolepis cirrhifer.

Science.gov (United States)

Fukano, Hanako; Wada, Shinpei; Kurata, Osamu; Katayama, Kinya; Fujiwara, Nagatoshi; Hoshino, Yoshihiko

2017-08-01

A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well as growth characteristics. Seven isolates were cultured from samples collected from five thread-sail filefish (Stephanolepis cirrhifer) and two farmed black scraper (Thamnaconus modestus). Bacterial growth occurred at 15-35 °C on Middlebrook 7H11 agar. The bacteria were positive for catalase activity at 68 °C and urease activity, intermediate for iron uptake, and negative for Tween 80 hydrolysis, nitrate reduction, semi-quantitative catalase activity and arylsulfatase activity at day 3. No growth was observed on Middlebrook 7H11 agar supplemented with picric acid, and very little growth was observed in the presence of 5 % NaCl. α- and α'-mycolates were identified in the cell walls, and a unique profile of the fatty acid methyl esters and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles of the protein and cell-wall lipids were acquired. Sequence analysis revealed that the seven isolates shared identical sequences for the 16S rRNA, rpoB, hsp65, recA and sodA genes. Phylogenetic analysis of the five gene sequences confirmed that the isolates were unique, but closely related to Mycobacterium chelonae. Antibiotic susceptibility testing revealed the minimum inhibitory concentration (MIC) of clarithromycin against this novel species was Mycobacterium salmoniphilum. The hsp65 PCR restriction enzyme analysis pattern differed from those of M. chelonae and M. salmoniphilum. Based on these findings, the name Mycobacterium stephanolepidis sp. nov. is proposed for this novel species, with the type strain being NJB0901 T (=JCM 31611 T =KCTC 39843 T ).

Complete Genome Sequence of Mycobacterium xenopi Type Strain RIVM700367

KAUST Repository

Abdallah, A. M.; Rashid, M.; Adroub, S. A.; Elabdalaoui, H.; Ali, Shahjahan; van Soolingen, D.; Bitter, W.; Pain, Arnab

2012-01-01

Mycobacterium xenopi is a slow-growing, thermophilic, water-related Mycobacterium species. Like other nontuberculous mycobacteria, M. xenopi more commonly infects humans with altered immune function, such as chronic obstructive pulmonary disease patients. It is considered clinically relevant in a significant proportion of the patients from whom it is isolated. We report here the whole genome sequence of M. xenopi type strain RIVM700367.

Complete Genome Sequence of Mycobacterium xenopi Type Strain RIVM700367

KAUST Repository

Abdallah, A. M.

2012-05-24

Mycobacterium xenopi is a slow-growing, thermophilic, water-related Mycobacterium species. Like other nontuberculous mycobacteria, M. xenopi more commonly infects humans with altered immune function, such as chronic obstructive pulmonary disease patients. It is considered clinically relevant in a significant proportion of the patients from whom it is isolated. We report here the whole genome sequence of M. xenopi type strain RIVM700367.

Detection of Mycobacterium chelonae, Mycobacterium abscessus Group, and Mycobacterium fortuitum Complex by a Multiplex Real-Time PCR Directly from Clinical Samples Using the BD MAX System.

Science.gov (United States)

Rocchetti, Talita T; Silbert, Suzane; Gostnell, Alicia; Kubasek, Carly; Campos Pignatari, Antonio C; Widen, Raymond

2017-03-01

A new multiplex PCR test was designed to detect Mycobacterium chelonae, Mycobacterium abscessus group, and Mycobacterium fortuitum complex on the BD MAX System. A total of 197 clinical samples previously submitted for mycobacterial culture were tested using the new protocol. Samples were first treated with proteinase K, and then each sample was inoculated into the BD MAX Sample Buffer Tube. Extraction and multiplex PCR were performed by the BD MAX System, using the BD MAX ExK TNA-3 extraction kit and BD TNA Master Mix, along with specific in-house designed primers and probes for each target. The limit of detection of each target, as well as specificity, was evaluated. Of 197 clinical samples included in this study, 133 were positive and 60 were negative for mycobacteria by culture, and another 4 negative samples were spiked with M. chelonae ATCC 35752. The new multiplex PCR on the BD MAX had 97% concordant results with culture for M. abscessus group detection, 99% for M. chelonae, and 100% for M. fortuitum complex. The new multiplex PCR test performed on the BD MAX System proved to be a sensitive and specific test to detect M. chelonae, M. abscessus group, and M. fortuitum complex by real-time PCR on an automated sample-in results-out platform. Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

Evaluation of GenoType NTM-DR Assay for Identification of Mycobacterium chimaera.

Science.gov (United States)

Mok, Simone; Rogers, Thomas R; Fitzgibbon, Margaret

2017-06-01

Identification of species within the Mycobacterium avium complex (MAC) is difficult, and most current diagnostic laboratory tests cannot distinguish between species included in the complex. Differentiation of species within the MAC is important, as Mycobacterium chimaera has recently emerged as a major cause of invasive cardiovascular infections following open heart surgery. A new commercial diagnostic assay, GenoType NTM-DR ver. 1.0, is intended to differentiate between three species within the MAC, namely, Mycobacterium avium , Mycobacterium intracellulare , and Mycobacterium chimaera In this study, we investigated an archival collection of 173 MAC isolates using 16S rRNA and 16S-23S internal transcribed spacer (ITS) gene sequencing, and GenoType NTM-DR was evaluated for identifying M. chimaera and other species belonging to the MAC. Species identification of 157/173 (91%) isolates with the GenoType NTM-DR assay was in agreement with 16S rRNA and 16S-23S ITS gene sequencing results. Misidentification occurred with 16 isolates which belonged to four species included in the MAC that are rarely encountered in clinical specimens. Despite some limitations of this assay, GenoType NTM-DR had 100% specificity for identifying M. chimaera This novel assay will enable diagnostic laboratories to differentiate species belonging to the Mycobacterium avium complex and to accurately identify M. chimaera It can produce rapid results and is also more cost efficient than gene sequencing methods. Copyright © 2017 American Society for Microbiology.

First description of Mycobacterium tuberculosis Beijing genotype in Argentina Primera descripción del genotipo Beijing de Mycobacterium tuberculosis en la Argentina

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N. Morcillo

2005-06-01

Full Text Available During a population-based study to genotype isolates of Mycobacterium tuberculosis from Buenos Aires Northern suburbs, we found isolates with molecular patterns related to those of the Beijing genotype. Five out of 590 (0.85% patients had isolates with spoligopattern identical to that of the Beijing family. Since two of these isolates showed identical IS6110RFLP pattern, we found only four different patterns containing 11 to 19 bands. The isolates were obtained from young people (including a 7 years-old child who were born in Argentina, and were living in a small area of our region. However, conventional contact tracing did not prove epidemiological linkage among them. These isolates were fully drug-susceptible to the first-line drugs. The comparison of the IS6110RFLP patterns from our isolates against a set of 19 reference Beijing patterns from the RIVM (The Netherlands confirmed that the strains belonged to the Beijing lineage. These findings might be partially explained by the important migration phenomena occurred during the last decade. Further surveillance studies would help in the following of Beijing family strain dissemination in our community.Durante un estudio poblacional para genotipificar aislamientos de Mycobacterium tuberculosis de Gran Buenos Aires Norte, se encontraron aislamientos con patrones moleculares relacionados con el genotipo Beijing. Cinco de 590 (0,85% pacientes tuvieron aislamientos con un patrón de spoligotyping idéntico al de la familia Beijing. Dos de esos aislamientos mostraron un patrón de IS6110 RFLP idéntico, por lo que finalmente fueron encontrados cuatro patrones diferentes conteniendo entre 11 y 19 bandas. Las cepas fueron aisladas de personas jóvenes incluyendo un niño de siete años de edad, todos ellos nacidos en Argentina y residentes en un pequeño distrito de nuestra región. Sin embargo, no pudo hallarse ninguna conexión epidemiológica entre ellos. La comparación de los patrones de IS6110

PURIFICACIÓN DEL ANTÍGENO 38 kDa DE Mycobacterium tuberculosis Y SU POTENCIAL USO EN DIAGNÓSTICO MEDIANTE INMUNOSENSORES PIEZOELÉCTRICOS

OpenAIRE

Paula A. Marín; Luz E. Botero; Jaime A. Robledo; Ana M. Murillo; Robinson A. Torres; Yeison J. Montagut; Elizabeth Pabón; Marisol Jaramillo

2015-01-01

Un paso crucial en el desarrollo de un inmunosensor piezoeléctrico para la detección de tuberculosis (TB), es la selección y obtención de los inmunoreactivos empleados en el inmunoensayo y la estrategia para la biofuncionalización del transductor. Diversos estudios han reportado el uso del antígeno proteico 38kDa (Ag38kDa) de Mycobacterium tuberculosis ( Mtb ) como un buen biomarcador de la enfermedad y el cumplimiento de las características físicas y bioquímicas para ser inmovilizado por mon...

PERFIL SOROLÓGICO EM EQUINOS INTOXICADOS EXPERIMENTALMENTE POR SENECIO BRASILIENSIS COMPOSITAE: PARTE II BILIRRUBINAS, ALBUMINAS E PROTEÍNAS

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Sonia Terezinha dos Anjos Lopes

1994-01-01

Full Text Available RESUMO As parte aéreas dessecadas de senecio brasiliensis, colhidas no período de brotação, foram administradas a 14 equinos de raça mista, com idade de 4 a 22 anos e pesos de 230 a 475kg. A planta era misturada à ração dada aos animais ou moída, misturada em água e administrada por sonda nasoesofágica. Foram realizadas colheitas de sangue periodicamente nos 14 equinos para avaliação dos níveis séricos de bilirrubina, albumina e proteína. Oito cavalos desenvolveram a intoxicação, três morreram por causas outras e três sobreviveram sem apresentar quaisquer sinais clínicos durante o período em que foram observados. Nos animais que desenvolveram a doença os sinais clínicos tiveram evolução de um a seis dias e caracterizaram-se por anorexia, icterícia e distúrbios neurológicos. A bilirrubina total e indireta apresentam-se elevadas na fase terminal da intoxicação e a albumina e proteína mostram níveis abaixo dos valores de referência num equino que desenvolveu a forma crônica da enfermidade. Conclui-se que dentre as provas laboratoriais usadas, a bilirrubina indireta apresenta melhor indicação da alteração hepática causada por intoxicação pelo senecio brasiliensis.

Endocardite em prótese biológica por candida parapsilosis

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Luiz Carlos Severo

1987-02-01

Full Text Available Relata-se um caso de endocardite causada pela Candida parapsilosis numa prótese valvular biológica em posição mitral. Revisa-se brevemente a literatura brasileira sobre o assunto.

Mycobacterium avium-intracellulare cellulitis occurring with septic arthritis after joint injection: a case report

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Murdoch David M

2007-02-01

Full Text Available Abstract Background Cellulitis caused by Mycobacterium avium-intracellulare has rarely been described. Mycobacterium avium-intracellulare is a rare cause of septic arthritis after intra-articular injection, though the causative role of injection is difficult to ascertain in such cases. Case presentation A 57-year-old with rheumatoid arthritis treated with prednisone and azathioprine developed bilateral painful degenerative shoulder arthritis. After corticosteroid injections into both acromioclavicular joints, he developed bilateral cellulitis centered over the injection sites. Skin biopsy showed non-caseating granulomas, and culture grew Mycobacterium avium-intracellulare. Joint aspiration also revealed Mycobacterium avium-intracellulare infection. Conclusion Although rare, skin and joint infections caused by Mycobacterium avium-intracellulare should be considered in any immunocompromised host, particularly after intra-articular injection. Stains for acid-fast bacilli may be negative in pathologic samples even in the presence of infection; cultures of tissue specimens should always be obtained.

Comparative genomics of archived pyrazinamide resistant Mycobacterium tuberculosis complex isolates from Uganda

Science.gov (United States)

Bovine tuberculosis is a ‘neglected zoonosis’ and its contribution to the proportion of Mycobacterium tuberculosis complex infections in humans is unknown. A retrospective study on archived Mycobacterium tuberculosis complex (MTC) isolates from a reference laboratory in Uganda was undertaken to iden...

Aciclovir: nuevo antiviral

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G. Repetto

2017-05-01

Full Text Available El aciclovir es un antiviral útil en infecciones graves causadas por el virus varicela-zoster. Es bien tolerado con escasas reacciones adversas. En pacientes deshidratados, en insuficiencia renal o si la infusión endovenosa es muy rápida, puede ocacionar una "nefropatía obstructiva" transitoria. Existen preparados de uso tópico, oftálmico, endovenoso y oral; esta última vía constituye una ventaja sobre la vidarabina con la que tiene en común el espectro de actividad. En razón de su selectividad, riesgo de resistencia y número reducido de antivirales, su prescripción debe restringirse a infecciones graves causadas por los agentes inmunodeprimidos; excluyendo por lo tanto las comunes y autolimitadas, frecuentes en el individuo normal.

Investigating Mycobacterium chelonae-abscessus Complex

Centers for Disease Control (CDC) Podcasts

2011-11-17

Keith Simmon, scientist at Isentio US discusses research that was done while he was at ARUP laboratories, discusses a new classification of Mycobacterium chelonae-abscessus complex.  Created: 11/17/2011 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 11/22/2011.

Characterization of Three Mycobacterium spp. with Potential Use in Bioremediation by Genome Sequencing and Comparative Genomics.

Science.gov (United States)

Das, Sarbashis; Pettersson, B M Fredrik; Behra, Phani Rama Krishna; Ramesh, Malavika; Dasgupta, Santanu; Bhattacharya, Alok; Kirsebom, Leif A

2015-06-16

We provide the genome sequences of the type strains of the polychlorophenol-degrading Mycobacterium chlorophenolicum (DSM43826), the degrader of chlorinated aliphatics Mycobacterium chubuense (DSM44219) and Mycobacterium obuense (DSM44075) that has been tested for use in cancer immunotherapy. The genome sizes of M. chlorophenolicum, M. chubuense, and M. obuense are 6.93, 5.95, and 5.58 Mb with GC-contents of 68.4%, 69.2%, and 67.9%, respectively. Comparative genomic analysis revealed that 3,254 genes are common and we predicted approximately 250 genes acquired through horizontal gene transfer from different sources including proteobacteria. The data also showed that the biodegrading Mycobacterium spp. NBB4, also referred to as M. chubuense NBB4, is distantly related to the M. chubuense type strain and should be considered as a separate species, we suggest it to be named Mycobacterium ethylenense NBB4. Among different categories we identified genes with potential roles in: biodegradation of aromatic compounds and copper homeostasis. These are the first nonpathogenic Mycobacterium spp. found harboring genes involved in copper homeostasis. These findings would therefore provide insight into the role of this group of Mycobacterium spp. in bioremediation as well as the evolution of copper homeostasis within the Mycobacterium genus. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

A Dermal Piercing Complicated by Mycobacterium fortuitum

Science.gov (United States)

Scroggins-Markle, Leslie; Kelly, Brent

2013-01-01

Background. Dermal piercings have recently become a fashion symbol. Common complications include hypertrophic scarring, rejection, local infection, contact allergy, and traumatic tearing. We report a rare case of Mycobacterium fortuitum following a dermal piercing and discuss its medical implications and treatments. Case. A previously healthy 19-year-old woman presented complaining of erythema and edema at the site of a dermal piercing on the right fourth dorsal finger. She was treated with a 10-day course of trimethoprim-sulfamethoxazole and one course of cephalexin by her primary care physician with incomplete resolution. The patient stated that she had been swimming at a local water park daily. A punch biopsy around the dermal stud was performed, and cultures with sensitivities revealed Mycobacterium fortuitum. The patient was treated with clarithromycin and ciprofloxacin for two months receiving full resolution. Discussion. Mycobacterium fortuitum is an infrequent human pathogen. This organism is a Runyon group IV, rapidly growing nontuberculous mycobacteria, often found in water,soil, and dust. Treatment options vary due to the size of the lesion. Small lesions are typically excised, while larger lesions require treatment for 2–6 months with antibiotics. We recommend a high level of suspicion for atypical mycobacterial infections in a piercing resistant to other therapies. PMID:24073343

A Dermal Piercing Complicated by Mycobacterium fortuitum

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Trisha Patel

2013-01-01

Full Text Available Background. Dermal piercings have recently become a fashion symbol. Common complications include hypertrophic scarring, rejection, local infection, contact allergy, and traumatic tearing. We report a rare case of Mycobacterium fortuitum following a dermal piercing and discuss its medical implications and treatments. Case. A previously healthy 19-year-old woman presented complaining of erythema and edema at the site of a dermal piercing on the right fourth dorsal finger. She was treated with a 10-day course of trimethoprim-sulfamethoxazole and one course of cephalexin by her primary care physician with incomplete resolution. The patient stated that she had been swimming at a local water park daily. A punch biopsy around the dermal stud was performed, and cultures with sensitivities revealed Mycobacterium fortuitum. The patient was treated with clarithromycin and ciprofloxacin for two months receiving full resolution. Discussion. Mycobacterium fortuitum is an infrequent human pathogen. This organism is a Runyon group IV, rapidly growing nontuberculous mycobacteria, often found in water,soil, and dust. Treatment options vary due to the size of the lesion. Small lesions are typically excised, while larger lesions require treatment for 2–6 months with antibiotics. We recommend a high level of suspicion for atypical mycobacterial infections in a piercing resistant to other therapies.

Molecular analysis of clinical isolates previously diagnosed as Mycobacterium intracellulare reveals incidental findings of "Mycobacterium indicus pranii" genotypes in human lung infection.

Science.gov (United States)

Kim, Su-Young; Park, Hye Yun; Jeong, Byeong-Ho; Jeon, Kyeongman; Huh, Hee Jae; Ki, Chang-Seok; Lee, Nam Yong; Han, Seung-Jung; Shin, Sung Jae; Koh, Won-Jung

2015-09-30

Mycobacterium intracellulare is a major cause of Mycobacterium avium complex lung disease in many countries. Molecular studies have revealed several new Mycobacteria species that are closely related to M. intracellulare. The aim of this study was to re-identify and characterize clinical isolates from patients previously diagnosed with M. intracellulare lung disease at the molecular level. Mycobacterial isolates from 77 patients, initially diagnosed with M. intracellulare lung disease were re-analyzed by multi-locus sequencing and pattern of insertion sequences. Among the 77 isolates, 74 (96 %) isolates were designated as M. intracellulare based on multigene sequence-based analysis. Interestingly, the three remaining strains (4 %) were re-identified as "Mycobacterium indicus pranii" according to distinct molecular phylogenetic positions in rpoB and hsp65 sequence-based typing. In hsp65 sequevar analysis, code 13 was found in the majority of cases and three unreported codes were identified. In 16S-23S rRNA internal transcribed spacer (ITS) sequevar analysis, all isolates of both species were classified within the Min-A ITS sequevar. Interestingly, four of the M. intracellulare isolates harbored IS1311, a M. avium-specific element. Two of three patients infected with "M. indicus pranii" had persistent positive sputum cultures after antibiotic therapy, indicating the clinical relevance of this study. This analysis highlights the importance of precise identification of clinical isolates genetically close to Mycobacterium species, and suggests that greater attention should be paid to nontuberculous mycobacteria lung disease caused by "M. indicus pranii".

Combating highly resistant emerging pathogen Mycobacterium abscessus and Mycobacterium tuberculosis with novel salicylanilide esters and carbamates.

Science.gov (United States)

Baranyai, Zsuzsa; Krátký, Martin; Vinšová, Jarmila; Szabó, Nóra; Senoner, Zsuzsanna; Horváti, Kata; Stolaříková, Jiřina; Dávid, Sándor; Bősze, Szilvia

2015-08-28

In the Mycobacterium genus over one hundred species are already described and new ones are periodically reported. Species that form colonies in a week are classified as rapid growers, those requiring longer periods (up to three months) are the mostly pathogenic slow growers. More recently, new emerging species have been identified to lengthen the list, all rapid growers. Of these, Mycobacterium abscessus is also an intracellular pathogen and it is the most chemotherapy-resistant rapid-growing mycobacterium. In addition, the cases of multidrug-resistant Mycobacterium tuberculosis infection are also increasing. Therefore there is an urgent need to find new active molecules against these threatening strains. Based on previous results, a series of salicylanilides, salicylanilide 5-chloropyrazinoates and carbamates was designed, synthesized and characterised. The compounds were evaluated for their in vitro activity on M. abscessus, susceptible M. tuberculosis H37Rv, multidrug-resistant (MDR) M. tuberculosis MDR A8, M. tuberculosis MDR 9449/2006 and on the extremely-resistant Praha 131 (XDR) strains. All derivatives exhibited a significant activity with minimum inhibitory concentrations (MICs) in the low micromolar range. Eight salicylanilide carbamates and two salicylanilide esters exhibited an excellent in vitro activity on M. abscessus with MICs from 0.2 to 2.1 μM, thus being more effective than ciprofloxacin and gentamicin. This finding is potentially promising, particularly, as M. abscessus is a threateningly chemotherapy-resistant species. M. tuberculosis H37Rv was inhibited with MICs from 0.2 μM, and eleven compounds have lower MICs than isoniazid. Salicylanilide esters and carbamates were found that they were effective also on MDR and XDR M. tuberculosis strains with MICs ≥1.0 μM. The in vitro cytotoxicity (IC50) was also determined on human MonoMac-6 cells, and selectivity index (SI) of the compounds was established. In general, salicylanilide

Mycobacterium smegmatis Has Two Pyrazinamidase Enzymes, PncA and PzaA

OpenAIRE

Guo, Ming; Sun, Zhonghe; Zhang, Ying

2000-01-01

The Mycobacterium smegmatis pncA gene, encoding nicotinamidase/pyrazinamidase, was identified. While it was similar to counterparts from other mycobacteria, the M. smegmatis PncA had little homology to the other M. smegmatis pyrazinamidase/nicotinamidase, encoded by the pzaA gene. Transformation of Mycobacterium bovis strain BCG with M. smegmatis pncA or pzaA conferred susceptibility to pyrazinamide.

Mycobacterium smegmatis has two pyrazinamidase enzymes, PncA and pzaA.

Science.gov (United States)

Guo, M; Sun, Z; Zhang, Y

2000-07-01

The Mycobacterium smegmatis pncA gene, encoding nicotinamidase/pyrazinamidase, was identified. While it was similar to counterparts from other mycobacteria, the M. smegmatis PncA had little homology to the other M. smegmatis pyrazinamidase/nicotinamidase, encoded by the pzaA gene. Transformation of Mycobacterium bovis strain BCG with M. smegmatis pncA or pzaA conferred susceptibility to pyrazinamide.

Buruli Ulcer (Mycobacterium ulcerans Infection)

Science.gov (United States)

... detail/buruli-ulcer-(mycobacterium-ulcerans-infection)","@context":"http://schema.org","@type":"Article"}; العربية 中文 français русский español ... Buruli ulcer on a regular basis to share information, coordinate disease control and research efforts, and monitor ...

Obtención y evaluación de un derivado proteico purificado de una cepa argentina de Mycobacterium avium subsp. paratuberculosis Production and evaluation of a purified protein derivative from an Argentine strain of Mycobacterium avium subsp. paratuberculosis (MAP

Directory of Open Access Journals (Sweden)

Andrea Gioffré

2012-09-01

Full Text Available Los derivados proteicos purificados (PPD son mezclas antigénicas no definidas obtenidas de distintas micobacterias. Los PPD bovino (PPDb y PPD aviar (PPDa son los antígenos que se emplean para evaluar la respuesta inmunitaria celular en infecciones como tuberculosis y paratuberculosis en el bovino. El PPDa comercial se produce a partir de Mycobacterium avium subsp. avium, y no a partir de la subespecie paratuberculosis. En este trabajo se seleccionó una cepa local de Mycobacterium avium subsp. paratuberculosis cuyo patrón molecular por RFLP es el más frecuente entre los aislamientos de nuestro país que han sido estudiados, y a partir de esta, se obtuvo un derivado proteico purificado: PPDj-IB. Se emplearon tanto el PPDa comercial como el PPDj-IB como antígenos en la prueba de liberación de gamma-interferón en animales de un tambo con paratuberculosis y en animales control. Aun cuando ambos PPD fueron capaces de estimular diferencialmente la liberación de la citoquina en el tambo infectado (respecto de los tambos control, no hubo diferencias significativas en los niveles de estimulación producidos y solo dos animales fueron positivos mediante el empleo de PPDj-IB. A partir del análisis por Western blot se demostró que el contenido de lipoarabinomano y del antígeno Apa/ModD era distinto en los PDD evaluados. Estas diferencias podrían explicar, en parte, las diferencias en los niveles de estimulación en términos individuales. Si bien el empleo de PPDj-IB no mejoró significativamente los resultados de la prueba de liberación de ?IFN, es importante destacar que se logró producir en el laboratorio un PPD apto para su empleo en ensayos in vitro.Purified Protein Derivatives (PPDs are non-defined antigens prepared from mycobacteria cultures. They are usually employed to evaluate the specific cellular immune response both in animals and humans. Bovine and avian PPDs are usually employed as antigens in mycobacterial infections such as

Analysis of the leprosy agents Mycobacterium leprae and Mycobacterium lepromatosis in four countries.

Science.gov (United States)

Han, Xiang Y; Aung, Fleur M; Choon, Siew Eng; Werner, Betina

2014-10-01

To differentiate the leprosy agents Mycobacterium leprae and Mycobacterium lepromatosis and correlate them with geographic distribution and clinicopathologic features. Species-specific polymerase chain reactions were used to detect each bacillus in archived skin biopsy specimens from patients with leprosy from Brazil (n = 52), Malaysia (n = 31), Myanmar (n = 9), and Uganda (n = 4). Findings were correlated with clinical and pathologic data. Etiologic species was detected in 46 of the 52 Brazilian patients, including 36 patients with M leprae, seven with M lepromatosis, and three with both bacilli. The seven patients with sole M lepromatosis all had tuberculoid leprosy, whereas only nine of the 36 patients infected with M leprae exhibited this type, and the rest were lepromatous (P leprae and two with M lepromatosis. Of the Malaysian and Ugandan patients, only M leprae was detected in 27 of the 31 Malaysians and two of the four Ugandans. The leprosy agents vary in geographic distribution. Finding M lepromatosis in Brazil and Myanmar suggests wide existence of this newly discovered species. The leprosy manifestations likely vary with the etiologic agents. Copyright© by the American Society for Clinical Pathology.

Evaluación de la mutagenicidad causada por metales pesados presentes en agua del río Cauca en la ciudad de Cali, Colombia

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Aura H. Vivas

2014-05-01

Full Text Available Se evaluó la mutagenicidad del agua del río Cauca debida a la presencia de metales pesados en la zona urbana de la Ciudad de Santiago de Cali, a partir de muestras tomadas en la temporada seca y lluviosa en el año 2013. Los metales se extrajeron pasando el agua por la resina Amberlite XAD-16. Las concentraciones de los metales pesados se midieron por absorción atómica y la mutagenicidad se evaluó por medio del test de Ames, con las cepas TA98 y TA100 de Salmonella typhimurium con y sin activador enzimático S9. Los resultados mostraron índices de mutagenicidad (IM positivos (IM > 2,0 para muestras colectadas en temporada lluviosa en tres de los cinco puntos evaluados: puente El Hormiguero (IM = 3,6, desembocadura del Canal Colector Sur (IM = 2,9 y desembocadura del río Cali (IM = 2,7, todos con la cepa TA98 sin S9. Estos sitios presentaron a su vez las mayores concentraciones totales de metales pesados en sus extractos. El análisis de la variación espacio-temporal del índice mutagénico se realizó haciendo un análisis de varianza multifactorial del IM. Los resultados encontrados indican que la época de muestreo contribuye significativamente a la variabilidad del IM, mientras que los puntos de muestreo no.

Un caso raro de valvulopatía causada por alcaptonuria

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Sara de Cima

2018-01-01

Full Text Available La alcaptonuria es una enfermedad rara autosómica recesiva del metabolismo de la tirosina que provoca el acúmulo de ácido homogentísico en determinados tejidos del cuerpo humano, como el tejido cartilaginoso, causando calcificación, degeneración y fibrosis. Las manifestaciones cardiovasculares son poco frecuentes, habiendo pocos casos descritos en la literatura en los que se produce afectación valvular cardíaca. Presentamos el caso de una mujer de 76 años con historia de alcaptonuria que fue diagnosticada de doble lesión aórtica severa e insuficiencia mitral moderada, requiriendo intervención quirúrgica para recambio valvular aórtico y mitral. Revisamos la etiología, la patogénesis, las manifestaciones clínicas, el diagnóstico y el tratamiento de esta patología.

par genes in Mycobacterium bovis and Mycobacterium smegmatis are arranged in an operon transcribed from "SigGC" promoters

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Casart Yveth

2008-03-01

Full Text Available Abstract Background The ParA/Soj and ParB/Spo0J proteins, and the cis-acting parS site, participate actively in chromosome segregation and cell cycle progression. Genes homologous to parA and parB, and two putative parS copies, have been identified in the Mycobacterium bovis BCG and Mycobacterium smegmatis chromosomes. As in Mycobacterium tuberculosis, the parA and parB genes in these two non-pathogenic mycobacteria are located near the chromosomal origin of replication. The present work focused on the determination of the transcriptional organisation of the ~6 Kb orf60K-parB region of M. bovis BCG and M. smegmatis by primer extension, transcriptional fusions to the green fluorescence protein (GFP and quantitative RT-PCR. Results The parAB genes were arranged in an operon. However, we also found promoters upstream of each one of these genes. Seven putative promoter sequences were identified in the orf60K-parB region of M. bovis BCG, whilst four were identified in the homologous region of M. smegmatis, one upstream of each open reading frame (ORF. Real-time PCR assays showed that in M. smegmatis, mRNA-parA and mRNA-parB levels decreased between the exponential and stationary phases. In M. bovis BCG, mRNA-parA levels also decreased between the exponential and stationary phases. However, parB expression was higher than parA expression and remained almost unchanged along the growth curve. Conclusion The majority of the proposed promoter regions had features characteristic of Mycobacterium promoters previously denoted as Group D. The -10 hexamer of a strong E. coli σ70-like promoter, located upstream of gidB of M. bovis BCG, overlapped with a putative parS sequence, suggesting that the transcription from this promoter might be regulated by the binding of ParB to parS.

Diffuse Lepromatous Leprosy Due to Mycobacterium lepromatosis in Quintana Roo, Mexico.

Science.gov (United States)

Han, Xiang Y; Quintanilla, Marco

2015-11-01

A 43-year-old woman of Mayan origin from Quintana Roo, Mexico, was diagnosed with diffuse lepromatous leprosy. The etiologic bacillus was determined to be Mycobacterium lepromatosis instead of Mycobacterium leprae. This case likely represents the first report of this leprosy form and its agent in the southeastern tip of Mexico. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Luxação traumática do bulbo ocular causada por acidente doméstico Traumatic eyeball luxation caused by domestic accident

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Rogério de Almeida Tárcia

2007-12-01

Full Text Available A luxação do bulbo ocular ocorre quando há seu deslocamento anterior, de tal forma que as pálpebras se fecham espasmodicamente por detrás dele. Diversas causas não traumáticas são relatadas, sendo que, quando provocado por traumas contusos, com grande envolvimento do nervo óptico e da musculatura extrínseca ocular, o prognóstico em geral é desfavorável. Apresentamos um dramático caso de luxação do bulbo ocular em criança após acidente doméstico, destacando seu mecanismo fisiopatológico e apreciando as condutas.The eyeball luxation is a dramatic phenomenon in which there is a forward displacement of the eyeball so that the eyelids spasmodically close behind it. There are many risk factors, non-related to trauma, but orbital contusions with optic nerve and extraocular muscles involvement carry the worst prognosis. The present paper reports a dramatic domestic case of globe luxation in childhood, its pathophysiology mechanisms and management.

Hiperinfestação por Strongyloides Stercoralis - doi:10.5020/18061230.2006.p118

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Tânia Maria Cavalcante Maia

2012-01-01

Full Text Available A estrongiloidíase é uma helmintíase predominantemente intestinal causada pelo Strongyloides stercoralis, sendo o homem seu hospedeiro definitivo, além da principal fonte de infecção. Este trabalho objetivou relatar um caso de identificação laboratorial de larva de Strongyloides stercolaris no líquido ascítico de um paciente do sexo masculino, 49 anos, agricultor, etilista há mais de 30 anos, encaminhado ao Hospital Universitário Walter Cantídio, por estar evoluindo com episódios de icterícia associada a aumento do volume abdominal diagnosticado como ascite, vômito, diarréia pastosa e diminuição do volume urinário. No internamento, foi iniciada medicação profilática para Peritonite Bacteriana Espontânea (PBE, bem como reposição de vitaminas, hidratação venosa e diuréticos, sem sucesso terapêutico. A partir de achados no líquido ascítico de presença de larvas de Strongyloides stercolaris em atividade, o quadro foi interpretado como estrongiloidíase disseminada em paciente imunocomprometido por hepatopatia avançada e alcoólica. O paciente evoluiu com quadro de choque caracterizado por hipotensão e hipotermia, tendo chegado a fazer uso de drogas vasoativas de forma não responsiva. O quadro evoluiu para óbito. Os autores chamam a atenção para a importância do diagnóstico e tratamento precoce da estrongiloidíase, principalmente em pacientes imunodeprimidos.

Multifaceted role of lipids in Mycobacterium leprae.

Science.gov (United States)

Kaur, Gurkamaljit; Kaur, Jagdeep

2017-03-01

Mycobacterium leprae must adopt a metabolic strategy and undergo various metabolic alterations upon infection to survive inside the human body for years in a dormant state. A change in lipid homeostasis upon infection is highly pronounced in Mycobacterium leprae. Lipids play an essential role in the survival and pathogenesis of mycobacteria. Lipids are present in several forms and serve multiple roles from being a source of nutrition, providing rigidity, evading the host immune response to serving as virulence factors, etc. The synthesis and degradation of lipids is a highly regulated process and is the key to future drug designing and diagnosis for mycobacteria. In the current review, an account of the distinct roles served by lipids, the mechanism of their synthesis and degradation has been elucidated.

Radiographic differentiation of atypical tuberculosis from mycobacterium tuberculosis

International Nuclear Information System (INIS)

Tarver, R.D.; Pearcy, E.A.; Conces, D.J. Jr.; Mathur, P.N.

1987-01-01

The chest radiographs of 95 patients with the new diagnosis of atypical turberculosis were reviewed to determine if any significant differences between atypical tuberculosis and that caused by Mycobacterium tuberculosis could be discerned. Findings included upper lobe involvement in B4 of the 95 patients and cavities in 76, with nearly equal groups having no, moderate, or extensive surrounding alveolar disease. Nodules were common; in six patients a nodule was the sole manifestation of disease. Adenopathy was seen in 12 of the 95 patients, atlectasis in 45, pleural thickening in 90, and effusions in three. These radiographic findings did not allow the radiographic differentiation of atypical tuberculosis from Mycobacterium tuberculosis infection

Lepra: Sepsis en un paciente con reacción tipo II. Reporte de un caso

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Edinson Enrique Escalante Gómez

2012-08-01

Full Text Available La lepra es una patología infecciosa de carácter crónico, caracterizada por un amplio compromiso cutáneo, asociado a neuropatía, con bajas tasas de mortalidad, pero con un alto índice de discapacidad. Es causada por la infección por el bacilo, Mycobacterium leprae. A pesar que se conoce desde la antigüedad se considera un problema persistente de salud pública en áreas subtropicales donde es endémica. Su mecanismo de transmisión, es por medio de gotitas respirato-rias. Su espectro clínico es muy variado, depende de la forma en que el sistema inmunitario del huésped reacciona frente al agente infeccioso, por lo que se reconocen los polos determinados como tuberculoide y lepromatoso, una forma indeterminada y borderline. Por otro lado se considera que la reacciones lepróticas (tipo 1, tipo 2 y fenómeno de lucio son complicaciones de la hiperreactividad inmunológica que aparece cuando se afecta el equilibrio inmunológico en el huésped. En 1982 la Organización Mundial de la Salud estableció la terapia multidrogas como herramienta eficaz para el control de esta entidad. Actualmente el régimen farmacológico se establece teniendo en cuenta la clasificación que distingue al enfermo en paucibacilar y multibacilar. Presentamos un paciente masculino de 64 años, con antecedente de lepra lepromatosa multibacilar desde hace 3 años, con tratamiento irregular, suspendido 6 meses antes de clínica de ingreso consistente sepsis de origen urinario, con posterior septicemia asociada al catéter, a quien se le realiza tratamiento antibiótico de amplio espectro, con evolución satisfactoria del estado hemodinámico y manejo de la lepra ambulatorio.

Mitogen-activated protein kinases mediate Mycobacterium

Indian Academy of Sciences (India)

CD44, an adhesion molecule, has been reported to be a binding site for Mycobacterium tuberculosis (M. tuberculosis) in macrophages and it also mediates mycobacterial phagocytosis, macrophage recruitment and protective immunity against pulmonary tuberculosis in vivo. However, the signalling pathways that are ...

21 CFR 866.3370 - Mycobacterium tuberculosis immunofluorescent reagents.

Science.gov (United States)

2010-04-01

... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents... used to identify Mycobacterium tuberculosis directly from clinical specimens. The identification aids...

Mycobacterium chelonae empyema with bronchopleural fistula in an immunocompetent patient

International Nuclear Information System (INIS)

Wali, Siraj

2009-01-01

Mycobacterium Calhoun is one of the rapidly growing mycobacteria that rarely cause lung disease. M chelonae more commonly causes skin and soft tissue infections primarily in immunosuppressed individuals. Thoracic empyema caused by rapidly growing mycobacteria and complicated with bronchopleural fistula is rarely reported, especially in immunocompetent patients. In this article we report the first immunocompetent Arabian patient presented with M chelonae- related empyema with bronchopleural fistula which mimics, clinically and radiologically, empyema caused by Mycobacterium tuberculosis. (author)

Preparation of a working seed lot of BCG and quality control by PCR genotyping Preparación de un lote semilla de trabajo de BCG y control de calidad por genotipificación por PCR

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A Trovero

2010-02-01

Full Text Available The bacillus Calmette-Guérin (BCG was obtained in 1920 after successive passages leading to the attenuation of a Mycobacterium bovis strain. For the following 40 years, BCG had been replicated, resulting in substrains with genotypic and phenotypic differences. Several genomic studies have compared two BCG strains, M. bovis and Mycobacterium tuberculosis, and observed that deleted regions in the different strains could be related to differences in antigenic properties. In this work, a working seed lot was obtained from a lyophilized secondary seed lot from the BCG Pasteur strain 1173 P2 and genetically characterized. The genome was analyzed by PCR directed to five regions (RD1, RD2, RD14, RD15, DU2, using the seed lot and different available strains as templates. No genetic differences were found in the fragments studied as compared to the Pasteur strain. A total of 20 passages were carried out and no differences were found in the size of the fragments amplified by PCR. In conclusion, this method allows to control a working seed lot genotypically and to assess the stability of the BCG genome.El bacilo de Calmette-Guérin (BCG se obtuvo en 1920, después de sucesivos pasajes que llevaron a la atenuación de una cepa de Mycobacterium bovis. A lo largo de los 40 años subsiguientes la cepa BCG fue replicada y surgieron subcepas con diferencias fenotípicas y genotípicas. Se realizaron varios estudios de comparación genómica de diferentes cepas de BCG, M. bovis y Mycobacterium tuberculosis, y se observó que las deleciones de regiones en las diferentes cepas podrían estar relacionadas con diferencias en las propiedades antigénicas. En este trabajo se describe la preparación y caracterización genética de un lote semilla de trabajo obtenido a partir de un lote semilla secundaria liofilizado de la cepa BCG Pasteur 1173 P2. Se analizaron por PCR cinco regiones (RD1, RD2, RD14, RD15, DU2 en el lote semilla de trabajo utilizando como control las

Mycobacterium alsense sp. nov., a scotochromogenic slow grower isolated from clinical respiratory specimens

DEFF Research Database (Denmark)

Tortoli, Enrico; Richter, Elvira; Borroni, Emanuele

2016-01-01

. Mycobacterium asiaticum is the most closely related species on the basis of the 16S rRNA sequence (similarity 99.3%); the average nucleotide Identity between the genomes of the two species is 80.72%, clearly below the suggested cutoff (95-96%). The name M. alsense is proposed here for the new species......"Mycobacterium alsiense", although reported in 2007, has not been validly published so far. The polyphasic characterization of the three strains available so far led us to the conclusion that they represent a distinct species within the genus Mycobacterium. The proposed new species grows slowly...

Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.

Science.gov (United States)

Nouioui, Imen; Sangal, Vartul; Carro, Lorena; Teramoto, Kanae; Jando, Marlen; Montero-Calasanz, Maria Del Carmen; Igual, José Mariano; Sutcliffe, Iain; Goodfellow, Michael; Klenk, Hans-Peter

2017-12-01

Two rapidly growing mycobacteria with identical 16S rRNA gene sequences were the subject of a polyphasic taxonomic study. The strains formed a well-supported subclade in the mycobacterial 16S rRNA gene tree and were most closely associated with the type strain of Mycobacterium novocastrense. Single and multilocus sequence analyses based on hsp65, rpoB and 16S rRNA gene sequences showed that strains SN 1900 T and SN 1904 T are phylogenetically distinct but share several chemotaxonomic and phenotypic features that are are consistent with their classification in the genus Mycobacterium. The two strains were distinguished by their different fatty acid and mycolic acid profiles, and by a combination of phenotypic features. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values for strains SN 1900 T and SN 1904 T were 61.0 % and 94.7 %, respectively; in turn, the corresponding dDDH and ANI values with M. novocastrense DSM 44203 T were 41.4 % and 42.8 % and 89.3 % and 89.5 %, respectively. These results show that strains SN1900 T and SN 1904 T form new centres of taxonomic variation within the genus Mycobacterium. Consequently, strains SN 1900 T (40 T =CECT 8763 T =DSM 43219 T ) and SN 1904 T (2409 T =CECT 8766 T =DSM 43532 T ) are considered to represent novel species, for which the names Mycobacteriumlehmannii sp. nov. and Mycobacteriumneumannii sp. nov. are proposed. A strain designated as 'Mycobacteriumacapulsensis' was shown to be a bona fide member of the putative novel species, M. lehmannii.

Protective and therapeutic efficacy of Mycobacterium smegmatis expressing HBHA-hIL12 fusion protein against Mycobacterium tuberculosis in mice.

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Shanmin Zhao

Full Text Available Tuberculosis (TB remains a major worldwide health problem. The only vaccine against TB, Mycobacterium bovis Bacille Calmette-Guerin (BCG, has demonstrated relatively low efficacy and does not provide satisfactory protection against the disease. More efficient vaccines and improved therapies are urgently needed to decrease the worldwide spread and burden of TB, and use of a viable, metabolizing mycobacteria vaccine may be a promising strategy against the disease. Here, we constructed a recombinant Mycobacterium smegmatis (rMS strain expressing a fusion protein of heparin-binding hemagglutinin (HBHA and human interleukin 12 (hIL-12. Immune responses induced by the rMS in mice and protection against Mycobacterium tuberculosis (MTB were investigated. Administration of this novel rMS enhanced Th1-type cellular responses (IFN-γ and IL-2 in mice and reduced bacterial burden in lungs as well as that achieved by BCG vaccination. Meanwhile, the bacteria load in M. tuberculosis infected mice treated with the rMS vaccine also was significantly reduced. In conclusion, the rMS strain expressing the HBHA and human IL-12 fusion protein enhanced immunogencity by improving the Th1-type response against TB, and the protective effect was equivalent to that of the conventional BCG vaccine in mice. Furthermore, it could decrease bacterial load and alleviate histopathological damage in lungs of M. tuberculosis infected mice.

Aspectos patológicos da intoxicação por aceturato de diminazeno em camelídeos sul-americanos

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Josilene N. Seixas

Full Text Available RESUMO: Aceturato de diminazeno é um fármaco quimioterápico sintético comumente usado na medicina veterinária para o tratamento de doenças causadas por parasitos hematozoários. Entretanto, seu uso pode levar a efeitos colaterais, como alterações neurológicas graves e morte. A criação de camelídeos é uma atividade recente no Brasil, fazendo-se necessário conhecer mais sobre as doenças que acometem essas espécies. De dez camelídeos (seis lhamas e quatro alpacas da propriedade, seis tiveram sinais clínicos e, destes, apenas uma lhama com manifestações leves recuperou-se. Os sinais clínicos incluíam apatia, andar cambaleante, fraqueza, sialorreia, cabeça baixa e pendida lateralmente, dificuldade em levantar e dispneia, observados a partir de 18 horas após o uso do medicamento. À necropsia e ao exame histopatológico foram observadas alterações de encefalopatia hemorrágica bilateral e simétrica, mais graves em tronco encefálico e tálamo. Este trabalho descreve as principais lesões observadas em um surto de intoxicação por diminazeno em alpacas (Lama pacos e lhamas (Lama glama e alerta criadores e veterinários sobre o risco de intoxicação por aceturato de diminazeno em camelídeos sul americanos.

Intoxicação experimental por Indigofera suffruticosa em caprinos e ovinos

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Anna P.M. de Figueiredo

2012-02-01

Full Text Available Indigofera suffruticosa é uma planta invasora, ção. Em um caprino e um ovino foram realizados os testes que causa anemia hemolítica com hemoglobinúria em bo-de fragilidade osmótica, determinação de hemoglobina e vinos e, experimentalmente, anemia sem hemoglobinúria metemoglobina e pesquisa de corpúsculos de Heinz. Foi em cobaios. O objetivo deste trabalho foi determinar a comprovado que em caprinos e ovinos, I. suffruticosa cautoxicidade de I. suffruticosa para caprinos e ovinos. Par-sa anemia hemolítica sem hemoglobinúria com formação tes aéreas da planta foram administradas a seis caprinos de corpúsculos de Heinz. Os animais recuperaram-se da e quatro ovinos em doses diárias de 10, 20 e 40g por kg anemia, total ou parcialmente, mesmo com a continuidade de peso vivo, durante períodos de 2 a 24 dias. Foram ava-da administração da planta. Oito a 12 horas após a coleliados os parâmetros hematológicos (hematócrito, níveis ta observa-se pigmento azulado na urina. Sugere-se que o de hemoglobina e contagem de hemácias e foi coletada pigmento seja anilina ou algum metabolito dessa substânurina para urinálise e observação de variações na coloracia e que a anilina seja o princípio ativo responsável pela hemólise causada por I. suffruticosa.

Identificação do circovírus suíno tipo 2 por reação em cadeia da polimerase e por imunoistoquímica em tecidos suínos arquivados desde 1988 no Brasil

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Ciacci-Zanella Janice Reis

2006-01-01

Full Text Available A síndrome multissistêmica do definhamento dos suínos (SMDS é uma doença de importância econômica causada pelo circovírus suíno tipo 2 (PCV2. Uma pesquisa retrospectiva foi realizada em amostras de órgãos de suínos fixados em blocos de parafina arquivados, que haviam sido submetidos à Embrapa Suínos e Aves entre 1985 e 1998 para o diagnóstico histopatológico. Vinte e cinco casos foram selecionados com base nas lesões histológicas características da SMDS, tais como linfoadenopatia, pneumonia intersticial, hepatite e nefrite intersticial. A presença de PCV2 nos cortes histológicos foi pesquisada por reação em cadeia da polimerase interna (nested-PCR, na qual utilizou-se primers específicos para a seqüência da ORF2 do PCV2 e também por imunoistoquímica, utilizando um anticorpo monoclonal específico para o capsídeo do PCV2. O DNA viral e os antígenos específicos do PCV2 foram detectados em amostras de tecidos de dois dos 25 casos analisados, sendo um desses datado de 1988. Esses resultados indicam que o PCV2 já estava presente no Brasil desde 1988.

Detection of Mycobacterium avium subspecies in the gut associated lymphoid tissue of slaughtered rabbits.

Science.gov (United States)

Arrazuria, Rakel; Sevilla, Iker A; Molina, Elena; Pérez, Valentín; Garrido, Joseba M; Juste, Ramón A; Elguezabal, Natalia

2015-06-11

Rabbits are susceptible to infection by different species of the genus Mycobacterium. Particularly, development of specific lesions and isolation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis, both subspecies of the M. avium complex, has been reported in wildlife conditions. Although, rabbit meat production worldwide is 200 million tons per year, microbiological data on this source of meat is lacking and more specifically reports of mycobacterial presence in industrially reared rabbit for human consumption have not been published. To this end, we sought mycobacteria by microbiological and histopathological methods paying special attention to Mycobacterium avium subsp. paratuberculosis in rabbits from commercial rabbitries from the North East of Spain. M. avium subsp. paratuberculosis was not detected either by culture or PCR. However, Mycobacterium avium subsp. avium was detected in 15.15% (10/66) and Mycobacterium avium subsp. hominissuis was detected in 1.51% (1/66) of gut associated lymphoid tissue of sampled animals by PCR, whereas caecal contents were negative. 9% (6/66) of the animals presented gross lesions suggestive of lymphoid activation, 6% (4/66) presented granulomatous lesions and 3% (2/66) contained acid fast bacilli. Mycobacterial isolation from samples was not achieved, although colonies of Thermoactinomycetes sp. were identified by 16s rRNA sequencing in 6% (4/66) of sampled animals. Apparently healthy farmed rabbits that go to slaughter may carry M. avium subspecies in gut associated lymphoid tissue.

Meningoradiculitis due to Cryptococcus neofermans in an immunocompetent patient Meningorradiculite lombossacra causada por Cryptococcus neoformans em paciente imunocompetente

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Leonardo Deus-Silva

2004-03-01

Full Text Available Meningoradiculitis refers to combined involvement of meninges and nerve roots. The most frequent location is the lumbosacral region. Etiology is diverse, including inflammatory, infectious and neoplastic disorders. Meningoradiculitis is a rare form of involvement in cryptococcal infection. We describe a case of subacute lower limbs flaccid paresis diagnosed as lumbosacral meningoradiculitis in view of cerebrospinal fluid (CSF inflammatory changes and typical enhancement on MRI of lumbar spine. Cryptococcus neoformans was isolated from CSF. Extensive screening yielded no immunodeficiencies.Meningorradiculite refere-se ao envolvimento simultâneo das meninges e das raízes dos nervos. O local mais freqüentemente acometido é a região lombossacra. Patologias inflamatórias, infecciosas e neoplásicas são as causas mais freqüentes. Meningorradiculite é manifestação rara de infecção por Cryptococcus neoformans. Descrevemos um caso de paresia flácida dos membros inferiores, com diagnóstico de meningorradiculite lombossacra baseado nos achados clínicos, de ressonância magnética da coluna lombar e em alterações inflamatórias do líquido cefalorraqueano (LCR. Avaliação microbiológica do LCR revelou a presença de Cryptococcus neoformans e extensa investigação clínica e laboratorial excluiu imunodeficiências primárias e adquiridas.

Osteomyelitis Infection of Mycobacterium marinum: A Case Report and Literature Review

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Hao H. Nguyen

2015-01-01

Full Text Available Mycobacterium marinum (M. marinum is a ubiquitous waterborne organism that grows optimally at temperatures around 30°C. It is a nontuberculous Mycobacterium found in nonchlorinated water with worldwide prevalence. It is the most common atypical Mycobacterium that causes opportunistic infection in humans. M. marinum can cause superficial infections and localized invasive infections in humans, with the hands being the sites most frequently affected. It can cause skin lesions, which are either single, papulonodular lesions, confined to an extremity, or may resemble cutaneous sporotrichosis. This infection can also cause deeper infections including tenosynovitis, bursitis, arthritis, and osteomyelitis. Disseminated infections and visceral involvements have been reported in immunocompromised patients. We here report a case of severe deep soft tissue infection with necrotizing fasciitis and osteomyelitis of the left upper extremity (LUE caused by M. marinum in an immunocompromised patient.

Diferencias en la fagocitosis de Mycobacterium ´habana´ TMC 5135 en cultivos celulares de macrófagos murinos

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Iliana Valdés-Hernández

2018-04-01

Full Text Available La tuberculosis es uno de los mayores problemas que enfrenta la salud mundial en la actualidad. La única vacuna disponible contra esta enfermedad es la BCG, esta protege solo contra la tuberculosis grave de la infancia, lo cual plantea un reto en la búsqueda de nuevos candidatos vacunales. Teniendo en cuenta el antecedente protector de Mycobacterium ´habana´ contra la tuberculosis experimental, nos propusimos aportar elementos que avalen el empleo de M. ´habana´ TMC 5135 como candidato vacunal contra la tuberculosis, mediante estudios de infección en cultivos celulares de macrófagos murinos. Se caracterizó el proceso de fagocitosis de esta micobacteria por cultivos primarios de macrófagos peritoneales murinos y por la línea celular RAW 264.7, para lo cual se determinó el porcentaje de fagocitosis y el número fagocítico. El presente trabajo demostró que el proceso de fagocitosis de M. ´habana´ TMC 5135 está influenciado por la fuente celular empleada como célula hospedadora, así como por la carga bacteriana infectante y el tiempo de exposición a la misma. La presente investigación contribuye a la caracterización de la infección por esta micobacteria en sus principales células blanco de la inmunidad innata y traza el camino de futuras investigaciones para evaluar la activación de mecanismos efectores de la inmunidad innata frente a este candidato.

Importância econômica, epidemiologia e controle das intoxicações por plantas no Brasil

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Clarice R.M. Pessoa

2013-06-01

Full Text Available A importância econômica, epidemiologia e controle das intoxicações por plantas em animais domésticos no Brasil são revisadas. Com os dados dos laboratórios de diagnóstico de diferentes regiões do país, as perdas anuais por mortes de animais foram estimadas em 820.761 a 1.755.763 bovinos, 399.800 a 445.309 ovinos, 52.675 a 63.292 caprinos e 38.559 equinos. No Brasil, atualmente, o número de plantas tóxicas é de 131 espécies e 79 gêneros e aumenta permanentemente. No entanto, a maioria das perdas são causadas por poucas plantas, incluindo Palicourea marcgravii, Amorimia spp., Senecio spp., Pteridium aquilinum, Ateleia glazioviana e Cestrum laevigatum em bovinos, Brachiaria spp em bovinos e ovinos, Nierembergia veitchii, Mimosa tenuiflora e Ipomoea asarifolia em ovinos, plantas que contêm swainsonina (Ipomoea carnea, Turbina cordata e Sida carpinifolia em caprinos e Brachiaria humidicola e Crotalaria retusa em equinos. Os principais fatores epidemiológicos relacionados às intoxicações por plantas incluem palatabilidade, fome, sede, facilitação social, desconhecimento da planta, acesso a plantas tóxicas, dose tóxica, período de ingestão, variações de toxicidade e resistência/susceptibilidade dos animais às intoxicações. Quanto aos métodos de controle e profilaxia descrevem-se os resultados obtidos no Brasil com métodos recentemente desenvolvidos, incluindo controle biológico, aversão alimentar condicionada, utilização de variedades não tóxicas de forrageiras, utilização de animais resistentes às intoxicações e técnicas de indução de resistência.

Cellular immune responses to ESAT-6 discriminate between patients with pulmonary disease due to Mycobacterium avium complex and those with pulmonary disease due to Mycobacterium tuberculosis

DEFF Research Database (Denmark)

Lein, A D; von Reyn, C F; Ravn, P

1999-01-01

ESAT-6 (for 6-kDa early secreted antigenic target) is a secreted antigen found almost exclusively in organisms of the Mycobacterium tuberculosis complex. We compared in vitro gamma interferon (IFN-gamma) responses by peripheral blood mononuclear cells to this antigen in patients with pulmonary...... disease due to either Mycobacterium avium complex (MAC) or Mycobacterium tuberculosis with those in healthy, skin test-negative, control subjects. Significant IFN-gamma responses to ESAT-6 were detected in 16 (59%) of 27 M. tuberculosis pulmonary disease patients, 0 (0%) of 8 MAC disease patients, and 0...... (0%) of 8 controls. Significant IFN-gamma responses to M. tuberculosis purified protein derivative were detected in 23 (85%) of 27 M. tuberculosis disease patients, 2 (25%) of 8 MAC disease patients, and 5 (63%) of 8 healthy controls. M. avium sensitin was recognized in 24 (89%) of 27 M. tuberculosis...

Detección de la expansión del triplete (CTGn, en personas sanas y en familias afectadas por Distrofia Miotónica

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Y. Gómez

2001-07-01

Full Text Available La Distrofia Miotónica (DM es una enfermedad de herencia autosómica dominante causada por la expansión inestable de un triplete (CTGn en la región 3' no traducida (3'UTR, del gen de la miotonin protein kinasa (MPK que mapea en 19q13.3. Es un desorden multisistémico, caracterizado por miotonía, debilidad muscular, cataratas, defectos en la conducción cardiaca, retardo mental y atrofia testicular. La forma más común es la del adulto, cuya incidencia es de 1 en 8.000, de expresión es variable, anticipación e impronta genética y cuya prevención es difícil porque al inicio de los síntomas, se tiene descendencia en la mayoría de los casos. El triplete (CTGn presenta de 5 a 30 repeticiones en la población normal, los afectados presentan más de 50 repeticiones y la severidad de la DM se correlaciona con el número de tripletas presentes. Se diseñó un protocolo para el análisis de hasta 100 repeticiones CTG, basado en PCR, para realizar el tamizaje para detectar los alelos normales en la población y pequeñas expansiones en individuos afectados por DM.

Cerebral ischemia caused by Streptococcus bovis aortic endocarditis: case report Isquemia cerebral causada por endocardite aórtica pelo Streptococcus bovis: relato de caso

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Leopoldo Santos-Neto

2005-09-01

Full Text Available Cerebral ischemic processes associated with infective endocarditis caused by Streptococcus bovis are rare; only 2 cases having been reported. Here we report a case of a 50-year-old man with S. bovis endocarditis who presented signs of frontal, parietal and occipital lobe cerebral ischemia. This is the first case reported in which the presence of hemianopsia preceded the endocarditis diagnosis. Initially, the clinical manifestations suggested a systemic vasculitis. Later, vegetating lesions were identified in the aortic valve and S. bovis grew in blood cultures. Antibiotic use and aortic valve replacement eliminated the infection and ceased thromboembolic events. A videocolonoscopy examination revealed no mucosal lesions as a portal of entry in this case, although such lesions have been encountered in up to 70% of reported cases of S. bovis endocarditis.A associação de isquemia cerebral e endocardite por Streptococcus bovis é um evento raro, tendo sido publicados apenas 2 casos anteriormente. Nós relatamos o caso de um homem de 50 anos com endocardite por S. bovis que apresentou sinais isquêmicos nos lobos frontal, parietal e occipital. Este é o primeiro caso em que a hemianopsia precedeu o diagnóstico de endocardite. Inicialmente, o quadro foi confundido com vasculite. Posteriormente, foi confirmada a presença de vegetações na válvula aórtica e a hemocultura identificou S. bovis. Os eventos tromboembólicos foram controlados com o uso de antibióticos e a troca da válvula aórtica. Estudo videocolonoscópico não identificou nenhuma lesão, apesar de lesões colônicas serem descritas em até 70% dos casos de indivíduos com endocardite por S. bovis.

Prevalence of latent Mycobacterium tuberculosis infection in prisoners.

Science.gov (United States)

Navarro, Pedro Daibert de; Almeida, Isabela Neves de; Kritski, Afrânio Lineu; Ceccato, Maria das Graças; Maciel, Mônica Maria Delgado; Carvalho, Wânia da Silva; Miranda, Silvana Spindola de

2016-01-01

To determine the prevalence of and the factors associated with latent Mycobacterium tuberculosis infection (LTBI) in prisoners in the state of Minas Gerais, Brazil. This was a cross-sectional cohort study conducted in two prisons in Minas Gerais. Tuberculin skin tests were performed in the individuals who agreed to participate in the study. A total of 1,120 individuals were selected for inclusion in this study. The prevalence of LTBI was 25.2%. In the multivariate analysis, LTBI was associated with self-reported contact with active tuberculosis patients within prisons (adjusted OR = 1.51; 95% CI: 1.05-2.18) and use of inhaled drugs (adjusted OR = 1.48; 95% CI: 1.03-2.13). Respiratory symptoms were identified in 131 (11.7%) of the participants. Serological testing for HIV was performed in 940 (83.9%) of the participants, and the result was positive in 5 (0.5%). Two cases of active tuberculosis were identified during the study period. Within the prisons under study, the prevalence of LTBI was high. In addition, LTBI was associated with self-reported contact with active tuberculosis patients and with the use of inhaled drugs. Our findings demonstrate that it is necessary to improve the conditions in prisons, as well as to introduce strategies, such as chest X-ray screening, in order to detect tuberculosis cases and, consequently, reduce M. tuberculosis infection within the prison system. Determinar a prevalência e os fatores associados à infecção latente por Mycobacterium tuberculosis (ILTB) em pessoas privadas de liberdade no Estado de Minas Gerais. Estudo de coorte transversal realizado em duas penitenciárias em Minas Gerais. Foi realizada a prova tuberculínica nos indivíduos que aceitaram participar do estudo. Foram selecionados 1.120 indivíduos para a pesquisa. A prevalência da ILTB foi de 25,2%. Na análise multivariada, a ILTB esteve associada com relato de contato com caso de tuberculose ativa dentro da penitenciária (OR ajustada = 1,51; IC95%: 1

DIGITAL DETECTION SYSTEM DESIGN OF MYCOBACTERIUM TUBERCULOSIS THROUGH EXTRACTION OF SPUTUM IMAGE USING NEURAL NETWORK METHOD

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Franky Arisgraha

2012-01-01

Full Text Available Tuberculosis (TBC is an dangerous disease and many people has been infected. One of many important steps to control TBC effectively and efficiently is by increasing case finding using right method and accurate diagnostic. One of them is to detect Mycobacterium Tuberculosis inside sputum. Conventional detection of Mycobacterium Tuberculosis inside sputum can need a lot of time, so digitally detection method of Mycobacterium Tuberculosis was designed as an effort to get better result of detection. This method was designed by using combination between digital image processing method and Neural Network method. From testing report that was done, Mycobacterium can be detected with successful value reach 77.5% and training error less than 5%.

Degradation of phytosteril into androstenedione by mycobacterium SP-UV-8

International Nuclear Information System (INIS)

Yang Ying; Jiang Shaotong; Wei Zhaojun; Zhao Yanyan; Sunxiaoming

2009-01-01

The fermentation process of Mycobacterium sp-UV-8 was studied in batch system, and a kinetic model was proposed based on the Logistic and Leudeking-piret equations for microorganism growth, product formation and substrate consumption. Depending on the evaluated model parameters, the model appears to provide a reasonable description for the fermentation process,and the average relative error was no more than 7%. The calculated results of models were compared satisfactorily with experimental data, which offers assurance for the industrial design and production throught degradation of phytosterol by Mycobacterium sp-UV-8. (authors)

Serovars of Mycobacterium avium Complex isolated from patients in Denmark

DEFF Research Database (Denmark)

Askgaard, D. S.; Giese, Steen Bjørck; Thybo, S.

1994-01-01

Danish isolates of Mycobacterium avium complex were serotyped by the use of seroagglutination. The most prevalent serovars among patients with AIDS (n = 89) were 4 and 6, while among non-AIDS patients the most prevalent serovars were 1, 6, and 4, with no major differences between those in patients...... with pulmonary disease (n = 65) and those in patients with lymph node infection (n = 58). The results suggest a Scandinavian distribution of serovars with a predominance of serovar 6 and fail to demonstrate any selective protection against different serovars by Mycobacterium bovis ECG vaccination....

Triple valve endocarditis by mycobacterium tuberculosis. A case report

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Shaikh Quratulain

2012-09-01

Full Text Available Abstract Background Granulomas caused by Mycobacterium Tuberculosis have been observed at autopsy in the heart, pre-dominantly in the myocardium and endocardium, but rarely involving the coronary vessels and valvular structures. Mycobacterium tuberculosis valvular endocarditis is extremely rare, with most reports coming from autopsy series. Case presentation We report the case of a 17 year old immunocompetent girl who presented with history of fever, malaise, foot gangrene and a left sided hemiparesis. On investigation she was found to have infective endocarditis involving the aortic, mitral and tricuspid valves. She had developed a right middle cerebral artery stroke. She underwent dual valve replacement and tricuspid repair. The vegetations showed granulomatous inflammation but blood cultures and other biological specimen cultures were negative for any organisms. She was started on antituberculous treatment and anticoagulation. Conclusion This is the first reported case of triple valve endocarditis by Mycobacterium Tuberculosis in an immunocompetent host. Especially important is the fact that the right heart is involved which has been historically described in the setting of intravenous drug abuse. This implies that Tuberculosis should be considered in cases of culture negative endocarditis in endemic areas like Pakistan even in immunocompetent hosts.

DETECCIÓN Y CARACTERIZACIÓN POR MÉTODOS MOLECULARES DE AISLAMIENTOS COLOMBIANOS DE HERPESVIRUS BOVINO TIPO 1

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D. Piedrahita

2005-01-01

Full Text Available La Rinotraqueitis Infecciosa Bovina (RIB es una enfermedad, altamente contagiosa, de distribución mundial, de origen viral, causada por el Herpesvirus Bovino-1 (BoHV-1. Produce alteraciones en el sistema respiratorio y reproductivo, lo que la convierte en una enfermedad con un gran impacto económico para los sistemas de producción ganadera. Este trabajo tuvo como objetivo caracterizar, mediante técnicas de biología molecular, tres aislamientos colombianos del BoHV-1 (dos de la sabana de Bogotá y uno de los Llanos Orientales. Los aislamientos fueron analizados con las enzimas de restricción Bam HI, Bst EII, Eco RI Pst I y Hind III. En este estudio también se implementó una rápida, sensitiva y específica prueba de PCR para la detección de tres glicoproteínas de superficie del Herpesvirus Bovino-1 (BoHV-1, cuyos fragmentos fueron secuenciados, lo que permitió encontrar homologías del 100% comparadas con los reportes del Gene Bank. Por medio del análisis con la enzima de restricción Hind III se clasificaron los aislamientos de la sabana de Bogotá como subtipo BoHV-1.2a y el de los Llanos Orientales como subtipo BoHV-1.1.

DETECCIÓN Y CARACTERIZACIÓN POR MÉTODOS MOLECULARES DE AISLAMIENTOS COLOMBIANOS DE Herpesvirus bovino TIPO 1

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Vera V.

2005-11-01

Full Text Available La Rinotraqueitis Infecciosa Bovina (RIB es una enfermedad, altamente contagiosa, de distribución mundial, de origen viral, causada por el Herpesvirus Bovino-1 (BoHV-1. Produce alteraciones en el sistema respiratorio y reproductivo, lo que la convierte en una enfermedad con un gran impacto económico para los sistemas de producción ganadera. Este trabajo tuvo como objetivo caracterizar, mediante técnicas de biología molecular, tres aislamientos colombianos del BoHV-1 (dos de la sabana de Bogotá y uno de los Llanos Orientales. Los aislamientos fueron analizados con las enzimas de restricción Bam HI, Bst EII, Eco RI Pst I y Hind III. En este estudio también se implementó una rápida, sensitiva y específica prueba de PCR para la detección de tres glicoproteínas de superficie del Herpesvirus Bovino-1 (BoHV-1, cuyos fragmentos fueron secuenciados, lo que permitió encontrar homologías del 100% comparadas con los reportes del Gene Bank. Por medio del análisis con la enzima de restricción Hind III se clasificaron los aislamientos de la sabana de Bogotá como subtipo BoHV-1.2a y el de los Llanos Orientales como subtipo BoHV-1.1.

Otomastoiditis Caused by Mycobacterium abscessus, the Netherlands

NARCIS (Netherlands)

van Ingen, Jakko; Looijmans, Frank; Mirck, Piet; Dekhuijzen, Richard; Boeree, Martin; van Soolingen, Dick

2010-01-01

To the Editor: Nontuberculous mycobacteria (NTM) are increasingly recognized as human pathogens (1). Otomastoiditis is a rare extrapulmonary NTM disease type first described in 1976; Mycobacterium chelonae-M. abscessus group bacteria, which are rapidly growing NTM, are the most frequent causative

Variable host-pathogen compatibility in Mycobacterium tuberculosis.

NARCIS (Netherlands)

Gagneux, Sebastien; DeRiemer, Kathryn; Van, Tran; Kato-Maeda, Midori; Jong, Bouke C de; Narayanan, Sujatha; Nicol, Mark; Niemann, Stefan; Kremer, Kristin; Gutierrez, M Cristina; Hilty, Markus; Hopewell, Philip C; Small, Peter M

2006-01-01

Mycobacterium tuberculosis remains a major cause of morbidity and mortality worldwide. Studies have reported human pathogens to have geographically structured population genetics, some of which have been linked to ancient human migrations. However, no study has addressed the potential evolutionary

Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii

DEFF Research Database (Denmark)

Kjeldsen, Marianne Kirstine; Bek, Dorte; Rasmussen, Erik Michael

2009-01-01

A line probe assay (GenoType MTBC) was evaluated for species differentiation within the Mycobacterium tuberculosis complex (MTBC). We included 387 MTBC isolates, 43 IS6110 low-copy MTBC isolates, 28 clinical specimens with varying microscopy grade, and 30 isolates of non-tuberculous mycobacteria...

AIDS and lung infection by Mycobacterium xenopi. Role of Computed Tomography

International Nuclear Information System (INIS)

Viterbo, V.; Midiri, M.; Stellacci, G.; Angelelli, G.; Rotondo, A.; Carbonara, S.; Maggi, P.; Monno, L.

2000-01-01

Mycobacterium xenopi is one of the most common agents responsible for nontubercolar mycobacterial pulmonary disease on AIDS patients. These lesions have been studied with conventional radiography while CT has been used in patients with a specific mycobacterioses or non-AIDS pulmonary conditions from Mycobacterium xenopi. 12 AIDS patients were examined. They had pulmonary lesions from Mycobacterium xenopi, patients age ranged 30 to 46 years. All patients had CD4 blood levels lower than 250 cells/mL and Mycobacterium xenopi in the sputum. All patients underwent a standard chest radiograph and a CT examination. CT images were evaluated by three radiologists independently and the definitive diagnosis was made in the presence of a fourth radiologist. Chest CT showed parenchymal consolidation in 66% of cases, associated with bilateral basal bands in 16% of cases. Consolidation was unilateral in 41% of cases and most frequently involved the right lower lobe. Bilateral reticular interstitial involvement was seen in the patients (41%). Micro nodules in 1 patient (8%) and mediastinal adenopathy in 33% of cases. Two patients had pre-existing emphysema and 1 had bronchiectasis. The frequency of lung disease from Mycobacterium xenopi has increased because of the spreading of the HIV infection. Such lung lesions in AIDS patients are a specific in appearance and localization, which the clinical radiologist needs to consider to address treatment planning. The frequent finding of parenchymal consolidation and the absence of cavitary lesions may be referred to the poor capability of AIDS to produce an adequate inflammatory response. The lung lesions tend to distribute in the lower lobes unilaterally. Adenopathy was also a frequent finding. CT plays a fundamental role in studying the chest of these patients because it permits to locate lung lesions with higher accuracy than conventional radiography and to detect adenopathies, micronodules, reticular interstitial involvement and

Resistencia a corte paralela a la tensión de la fibra de la madera, por medio de la prueba de punzonamiento propuesta

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Mário Massayuki

2014-01-01

Full Text Available Esta investigación tiene como objetivo proponer un método para probar la resistencia a corte de la madera paralela a la fibra y realizar la comparación con otros métodos propuestos. El método propuesto por la Norma Brasileña (NBR 7190/1997 que es similar a la D 143-09/2010, utiliza una probeta prismática rectangular con una muesca para evitar su rotación, que es causada por la asimetría en la aplicación de la fuerza que promueve el corte. A fin de eliminar esta asimetría, en el procedimiento propuesto se aplica tensión de corte por la perforación de una simple muestra de ensayo prismática sin cortes, con la distribución de la sección de cizallamiento simétricamente en relación a un punto. Por consiguiente, se desarrolló una herramienta macho y hembra adaptada para una prensa de prueba universal de aplicación de la fuerza. Una vez verificada la consistencia del método, se ha llevado a cabo una serie de pruebas con tres tipos diferentes de madera, (Pinus sp, Erisma uncinatum y Mezilaurus itauba, que se distinguen por su resistencia mecánica, inspeccionando el método por el Santos Neto (1999 bajo la NBR 7190/1997 Norma Brasileña, y comparándolo con el método de punzonamiento - puncionado. Los resultados mostraron que en la prueba con la NBR 7190/1997, los métodos de la Norma Brasileña y el método propuesto muestran resultados similares y próximos entre ellos.

Isolation and Identification of Pyrene Mineralizing Mycobacterium spp. from Contaminated and Uncontaminated Sources

International Nuclear Information System (INIS)

Lease, C.W.M; Bentham, R.H; Gaskin, S.E; Juhasz, A.L

2011-01-01

Mycobacterium isolates obtained from PAH-contaminated and uncontaminated matrices were evaluated for their ability to degrade three-, four- and five-ring PAHs. PAH enrichment studies were prepared using pyrene and inocula obtained from manufacturing gas plant (MGP) soil, uncontaminated agricultural soil, and faeces from Macropus fuliginosus (Western Grey Kangaroo). Three pyrene-degrading microorganisms isolated from the corresponding enrichment cultures had broad substrate ranges, however, isolates could be differentiated based on surfactant, phenol, hydrocarbon and PAH utilisation. 16S rRNA analysis identified all three isolates as Mycobacterium sp. The Mycobacterium spp. could rapidly degrade phenanthrene and pyrene, however, no strain had the capacity to utilise fluorene or benzo[a]pyrene. When pyrene mineralisation experiments were performed, 70-79% of added 14 C was evolved as 14 CO 2 after 10 days. The present study demonstrates that PAH degrading microorganisms may be isolated from a diverse range of environmental matrices. The present study demonstrates that prior exposure to PAHs was not a prerequisite for PAH catabolic activity for two of these Mycobacterium isolates.

Mycobacterium alsense sp. nov., a scotochromogenic slow grower isolated from clinical respiratory specimens.

Science.gov (United States)

Tortoli, Enrico; Richter, Elvira; Borroni, Emanuele; Cabibbe, Andrea M; Capitolo, Eleonora; Cittaro, Davide; Engel, Regina; Hendricks, Oliver; Hillemann, Doris; Kristiansen, Jette E; Mariottini, Alessandro; Schubert, Sabine; Cirillo, Daniela M

2016-01-01

The name 'Mycobacterium alsiense', although reported in 2007, has not been validly published. Polyphasic characterization of three available strains of this species led us to the conclusion that they represent a distinct species within the genus Mycobacterium. The proposed novel species grows slowly and presents pale yellow-pigmented colonies. Differentiation from other mycobacteria is not feasible on the basis of biochemical and cultural features alone while genetic analysis, extended to eight housekeeping genes and one spacer region, reveals its clear distinction from all other mycobacteria. Mycobacterium asiaticum is the most closely related species on the basis of 16S rRNA gene sequences (similarity 99.3 %); the average nucleotide identity between the genomes of the two species is 80.72 %, clearly below the suggested cut-off (95-96 %). The name Mycobacterium alsense sp. nov. is proposed here for the novel species and replaces the name 'M. alsiense', ex Richter et al. 2007, given at the time of isolation of the first strain. The type strain is TB 1906T ( = DSM 45230T = CCUG 56586T).

Specific T-cell epitopes for immunoassay-based diagnosis of Mycobacterium tuberculosis infection

DEFF Research Database (Denmark)

Brock, I; Weldingh, K; Leyten, EM

2004-01-01

Specific T-cell epitopes for immunoassay-based diagnosis of Mycobacterium tuberculosis infection.Brock I, Weldingh K, Leyten EM, Arend SM, Ravn P, Andersen P. Department of Infectious Disease Immunology, Statens Serum Institute, Artillerivej 5, DK-2300 Copenhagen S, Denmark. The currently used...... method for immunological detection of tuberculosis infection, the tuberculin skin test, has low specificity. Antigens specific for Mycobacterium tuberculosis to replace purified protein derivative are therefore urgently needed. We have performed a rigorous assessment of the diagnostic potential of four...... recently identified antigens (Rv2653, Rv2654, Rv3873, and Rv3878) from genomic regions that are lacking from the Mycobacterium bovis bacillus Calmette-Guerin (BCG) vaccine strains as well as from the most common nontuberculous mycobacteria. The fine specificity of potential epitopes in these molecules...

Seroprevalence of Mycobacterium avium SSP paratuberculosis ...

African Journals Online (AJOL)

This study aimed to determine the seroprevalence of antibodies for Mycobacterium avium subspecies paratuberculosis (MAP) in dairy cattle in the Jimma zone of Ethiopia in 2011. A random sample of 29 herds was selected, and all mature cattle within these herds had a blood sample taken. Serum was tested in duplicate, ...

Mycobacterium aquiterrae sp. nov., a rapidly growing bacterium isolated from groundwater.

Science.gov (United States)

Lee, Jae-Chan; Whang, Kyung-Sook

2017-10-01

A strain representing a rapidly growing, Gram-stain-positive, aerobic, rod-shaped, non-motile, non-sporulating and non-pigmented species of the genus Mycobacterium, designated strain S-I-6 T , was isolated from groundwater at Daejeon in Korea. The strain grew at temperatures between 10 and 37 °C (optimal growth at 25 °C), between pH 4.0 and 9.0 (optimal growth at pH 7.0) and at salinities of 0-5 % (w/v) NaCl, growing optimally with 2 % (w/v) NaCl. Phylogenetic analyses based on multilocus sequence analysis of the 16S rRNAgene, hsp65, rpoB and the 16S-23S internal transcribed spacer indicated that strain S-I-6 T belonged to the rapidly growing mycobacteria, being most closely related to Mycobacterium sphagni. On the basis of polyphasic taxonomic analysis, the bacterial strain was distinguished from its phylogenetic neighbours by chemotaxonomic properties and other biochemical characteristics. DNA-DNA relatedness among strain S-I-6 T and the closest phylogenetic neighbour strongly support the proposal that this strain represents a novel species within the genus Mycobacterium, for which the name Mycobacterium aquiterrae sp. nov. is proposed. The type strain is S-I-6 T (=KACC 17600 T =NBRC 109805 T =NCAIM B 02535 T ).

ELECTROPHORETIC MOBILITY OF MYCOBACTERIUM AVIUM COMPLEX ORGANISMS

Science.gov (United States)

The electrophoretic mobilities (EPMs) of thirty Mycobacterium avium Complex (MAC) organisms were measured. The EPMs of fifteen clinical isolates ranged from -1.9 to -5.0 µm cm V-1s-1, and the EPMs of fifteen environmental isolates ranged from -1...

Pigmentos lipossolúveis e hidrossolúveis em plantas de salvínia sob toxicidade por cromo Liposoluble and hydrosoluble pigments in salvinia under chromium toxicity

Directory of Open Access Journals (Sweden)

P.F. Pereira

2012-12-01

Full Text Available Devido à intensa utilização industrial, o cromo é considerado um importante poluente ambiental. O presente trabalho objetivou determinar os teores de pigmentos hidro e lipossolúveis em plantas de salvínia expostas a concentrações crescentes de Cr, visando estabelecer parâmetros bioquímicos para utilização dessa macrófita em programas de biomonitoramento e/ou fitorremediação da poluição causada por esse poluente metálico em ambientes aquáticos. As plantas foram submetidas a concentrações crescentes de Cr e avaliadas após quatro, seis e dez dias de tratamento. Os resultados dos ensaios permitiram concluir que plantas de salvínia sob condições de estresse por Cr apresentam reduções nas concentrações das clorofilas a, b e total e, em contraste, aumentos nas concentrações de antocianinas totais. Embora a concentração de carotenoides totais não tenha sido alterada em resposta ao Cr, as variações nas concentrações dos demais pigmentos lipossolúveis e dos pigmentos hidrossolúveis observadas nas folhas das plantas de salvínia podem ser utilizadas como parâmetros bioquímicos de biomonitoramento da poluição causada por esse elemento metálico em ambientes aquáticos.Due to widespread industrial use, chromium is considered a serious environmental pollutant. This study aimed to determine the content of hydrosoluble and liposoluble pigments in salvinia plants exposed to increasing concentrations of Cr, to establish biochemical parameters for the use of macrophyta in pollution bio-monitoring programs and/or phyto-remediation in aquatic environments by this pollutant metal. The plants were exposed to increasing concentrations of Cr and evaluated after four, six, and ten days of treatment. The test results showed that salvinia plants under stress conditions for Cr exhibit decreases in the concentrations of chlorophylls a, b, and total, and, in contrast, increases in anthocyanin concentrations. Although the

El Blindaje Discursivo de las Muertes Causadas por el Amianto em Brasil: Criminología Crítica y Daño Social

Directory of Open Access Journals (Sweden)

Marília de Nardin Budó

2016-06-01

Full Text Available La limitación del sistema de control penal a la persecución de los crímenes callejeros exige una definición autónoma del objeto de la Criminología, alcanzando los daños sociales. El objetivo del trabajo es identificar el tratamiento del caso del amianto en Brasil y en el mundo, en lo que afecta al conflicto discursivo que oculta las incontables muertes que causa. Analiza, a través del método dialéctico, la apropiación del discurso médico por el Legislativo y por el Judiciario. Del contrapunto de las interpretaciones percibe-se la tentativa de un blindaje, que ultrapasa la ciencia para la conservación de los intereses del capital.

Multinucleated giant cell cytokine expression in pulmonary granulomas of cattle experimentally infected with Mycobacterium bovis

Science.gov (United States)

Pathogenic mycobacteria of the Mycobacterium tuberculosis complex such as Mycobacterium bovis, induce a characteristic lesion known as a granulomas. Granulomas represent a specific host response to chronic antigenic stimuli, such as foreign bodies, certain bacterial components, or persistent pathoge...

Mycobacterium alsiense, a novel, slowly growing species isolated from two patients with pulmonary disease

DEFF Research Database (Denmark)

Richter, Elvira; Tortoli, Enrico; Fischer, Arno

2007-01-01

A previously undescribed, slowly growing Mycobacterium species was isolated from pulmonary specimens of two patients, one from Denmark and one from Italy. The isolates showed unique 16S rRNA internal transcribed spacers and hsp65 sequences: the 16S rRNA was most closely related to Mycobacterium...

Lesões de pele causadas pelos espinhos de Mimosa pudica (Leg. Mimosoideae nos membros de bovinos e ovinos no estado do Pará Cutaneous lesions of the legs caused by the thorns of Mimosa pudica (Leg. Mimosoideae in cattle and sheep in the State of Pará

Directory of Open Access Journals (Sweden)

José Diomedes Barbosa

2009-05-01

Full Text Available Foi estudada uma doença em bovinos e ovinos caracterizada por lesões ulcerativas e granulomatosas da pele dos membros. Os estudos epidemiológicos e patológicos permitiram concluir que essas lesões são causadas pelos espinhos de Mimosa pudica (Leg. Mimosoideae. A doença foi observada somente em pastagens acentuadamente infestadas e os animais se recuperaram rapidamente após retirados destes pastos. Nos ovinos as lesões atingiam partes mais altas dos membros do que nos bovinos, devido ao seu menor porte.A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae. The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.

Control biológico de la Antracnosis causada por Colletotrichum gloeosporioides (Penz. y Sacc.) en Tomate de Árbol (Solanum betaceum Cav.) mediante hongos endófitos antagonistas

OpenAIRE

Delgado Fernández, Ernesto; Vásquez Matute, Santiago

2010-01-01

La antracnosis, en el tomate de árbol (Solanum betaceum) conocida por nuestros agricultores como “ojo de pollo” se produce por un hongo del género Colletotrichum Sp. es una enfermedad que causa pérdidas incalculables, y hasta el momento su control, se hace mediante productos químicos, que en algunos casos son nocivos y atentan contra el medio ambiente. El uso de microorganismos como inhibidores del desarrollo de microorganismos fitopatógenos, se presenta como una alternativa para ...

Progression to active tuberculosis, but not transmission, varies by Mycobacterium tuberculosis lineage in The Gambia

NARCIS (Netherlands)

de Jong, Bouke C.; Hill, Philip C.; Aiken, Alex; Awine, Timothy; Antonio, Martin; Adetifa, Ifedayo M.; Jackson-Sillah, Dolly J.; Fox, Annette; Deriemer, Kathryn; Gagneux, Sebastien; Borgdorff, Martien W.; McAdam, Keith P. W. J.; Corrah, Tumani; Small, Peter M.; Adegbola, Richard A.

2008-01-01

BACKGROUND: There is considerable variability in the outcome of Mycobacterium tuberculosis infection. We hypothesized that Mycobacterium africanum was less likely than M. tuberculosis to transmit and progress to tuberculosis disease. METHODS: In a cohort study of patients with tuberculosis and their

Proteasomal control of cytokinin synthesis protects Mycobacterium tuberculosis against nitric oxide

Science.gov (United States)

Samanovic, Marie I.; Tu, Shengjiang; Novák, Ondřej; Iyer, Lakshminarayan M.; McAllister, Fiona E.; Aravind, L.; Gygi, Steven P.; Hubbard, Stevan R.; Strnad, Miroslav; Darwin, K. Heran

2015-01-01

Summary One of several roles of the Mycobacterium tuberculosis proteasome is to defend against host-produced nitric oxide (NO), a free radical that can damage numerous biological macromolecules. Mutations that inactivate proteasomal degradation in Mycobacterium tuberculosis result in bacteria that are hypersensitive to NO and attenuated for growth in vivo, but it was not known why. To elucidate the link between proteasome function, NO-resistance, and pathogenesis, we screened for suppressors of NO hypersensitivity in a mycobacterial proteasome ATPase mutant and identified mutations in Rv1205. We determined that Rv1205 encodes a pupylated proteasome substrate. Rv1205 is a homologue of the plant enzyme LONELY GUY, which catalyzes the production of hormones called cytokinins. Remarkably, we report for the first time that an obligate human pathogen secretes several cytokinins. Finally, we determined that the Rv1205-dependent accumulation of cytokinin breakdown products is likely responsible for the sensitization of Mycobacterium tuberculosis proteasome-associated mutants to NO. PMID:25728768

The epidemiology of Mycobacterium leprae: recent insight

NARCIS (Netherlands)

van Beers, S. M.; de Wit, M. Y.; Klatser, P. R.

1996-01-01

Leprosy is still a health problem in many countries. Because the causative organism, Mycobacterium leprae cannot be cultured in vitro, it is virtually impossible to assess exposure, and the onset of infection and disease. As a consequence, the chain of infection, considered as the relationships

Detection of Mycobacterium Tuberculosis by using PCR

International Nuclear Information System (INIS)

Suhadi, F; Dadang-Sudrajat; Maria-Lina, R.

1996-01-01

Polymerase Chain Reaction (PCR) procedure using three primary set derived from repetitive DNA sequence specific to mycobacteria was used to diagnose pathogenic Mycobacterium tuberculosis. The assay was specific for M. tuberculosis and could be used to detect the amount DNA less than 10 -9 g

Modelling the Transitional Dynamics of Mycobacterium Tuberculosis ...

African Journals Online (AJOL)

The World Health Organization's targets of eliminating Tuberculosis (TB) by 2050 is challenged by the emergence and spread of drug resistance TB. However, the traditional mechanism of resistance is that of acquired resistance, whereby the mycobacterium Tuberculosis (MTB) strain develops mutations under selective ...

Putative in vitro expressed gene fragments unique to Mycobacterium avium subspecies para tuberculosis

DEFF Research Database (Denmark)

Nielsen, Kirstine Klitgaard; Ahrens, Peter

2002-01-01

By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. para tuberculosis (M. para tuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniquen......By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. para tuberculosis (M. para tuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria....... The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium (M. avium) was confirmed by species-specific PCR and Southern blot. Furthermore, RT-PCR indicated that eight of the nine fragments originate from areas...

Extra pulmonary tuberculosis: Rapid identification of Mycobacterium tuberculosis grown in Mycobacterium growth indicator tube 960 and Lowenstein-Jensen media, employing Standard diagnostics Bioline Mycobacterium tuberculosis protein 64 antigen detection kit

Directory of Open Access Journals (Sweden)

G Kandhakumari

2015-01-01

Full Text Available Background: Investigation of extra pulmonary tuberculosis (EPTB in and around Pondicherry is being carried out since August 2011 in our tertiary care super specialty hospital. Objectives: To compare the rapid Kit SD Bio-Line MPT 64 Ag with conventional and time consuming biochemical tests. Confirmation of Mycobacterium tuberculosis at a reasonable time frame is the main thrust. Materials and Methods: Thirty three Mycobacterium tuberculosis and four Non-Tuberculous Mycobacteria (NTM grown in MGIT960 system/Lowenstein-Jensen media (LJ were examined by the rapid MPT 64 antigen detection as well as a battery of conventional tests like niacin, nitrate reduction, paraminobenzoic acid susceptibility and cord formation. Results and Conclusion: . Both the rapid kit and conventional tests correctly identified 33 M.tuberculosis isolates. Keeping conventional identification as reference, sensitivity and specificity for rapid kit was 100%. Rapid kit which takes only 15 minutes is accurate, cost effective, and facilitates early treatment for these EPTB patients, whose clinical specimens are paucibacillary.

Peritoneal tuberculosis due to Mycobacterium caprae

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T. Nebreda

2016-01-01

Full Text Available The incidence of tuberculosis in humans due to Mycobacterium caprae is very low and is almost confined to Europe. We report a case of a previously healthy 41-year-old Moroccan with a 6 month history of abdominal pain, weight loss, fatigue and diarrhea. A diagnosis of peritoneal tuberculosis due to M. caprae was made.

Mycobacterium marinum infection in a blue-fronted Amazon parrot (Amazona aestiva).

Science.gov (United States)

Hannon, David E; Bemis, David A; Garner, Michael M

2012-12-01

A blue-fronted Amazon parrot (Amazona aestiva) was presented with a granuloma involving the proximal rhinotheca and extending into the rostral sinuses. Mycobacterium marinum was diagnosed based on results of biopsy and culture. Treatment was initiated with clarithromycin, rifampin, and ethambutol, but the bird died 4 months after the onset of antimicrobial therapy. Additional granulomas were found in the left lung and liver on postmortem examination. Mycobacterial isolation on postmortem samples was unsuccessful. This is the first report of Mycobacterium marinum in a bird.

Structural studies on Mycobacterium tuberculosis RecA

Indian Academy of Sciences (India)

Structures of crystals of Mycobacterium tuberculosis RecA, grown and analysed under different conditions, provide insights into hitherto underappreciated details of molecular structure and plasticity. In particular, they yield information on the invariant and variable features of the geometry of the P-loop, whose binding to ATP ...

Chronic leg ulcer caused by Mycobacterium immunogenum

NARCIS (Netherlands)

Loots, Miriam A. M.; de Jong, Menno D.; van Soolingen, Dick; Wetsteyn, José C. F. M.; Faber, William R.

2005-01-01

Rare tropical skin diseases are seen more frequently in Western countries because of the increased popularity of visiting tropical regions. A 55-year-old white man developed a painless leg ulcer after traveling in Guatemala and Belize. A mycobacterium was cultured from a biopsy specimen and was

Identification of Immunotopes against Mycobacterium leprae as ...

African Journals Online (AJOL)

Purpose: To determine the surface epitopes of Mycobacterium leprae (M. leprae) and evaluate their efficacy in the production of anti-M. leprae antibodies in an animal model. Methods: Blood samples were obtained from 34 patients suffering from lepromatous leprosy. Antibodies were obtained from the samples, ...

Meningitis neonatal e infección puerperal por Streptococcus pneumoniae: Presentación de un caso

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Cecilia Ortiz Rodríguez

2003-06-01

Full Text Available Las infecciones neonatales causadas por Streptococcus pneumoniae constituyen un fenómeno raro, poco reportado en la literatura médica; en el presente trabajo se describe el caso de una meningoencefalitis purulenta de aparición precoz, en un neonato nacido por parto eutócico, a termino y de buen peso. Se aisló el microorganismo en la sangre y en el líquido cefalorraquídeo del recién nacido, así como en los loquios de la madre. Las 3 antibiotipias fueron idénticas. La evolución del niño fue desfavorable, el cual falleció a las 81 horas de vida, en un cuadro de fallo multiorgánico. La necropsia corroboró el diagnóstico. La madre desarrolló una endometritis puerperal a los 4 días, con buena evolución.Neonatal infections caused by Streptococcus pneumoniae are a rare phenomenon that is barely reported in the medical literature. The present paper describes a case of early purulent meningoencephalitis occurred in an adequate birthweight neonate born to term eutocic delivery. The microorganism was isolated in the newborn's blood and cerebrospinal fluid as well as in his mother's lochia. The three antibiotypes were identical. The newborn did not recover and died after 81 hours due to a multiple organ failure. Necropsy confirmed the diagnosis. The mother developed puerperal endometritis after 4 days but did recover.

Mycobacterium spp. in wild game in Slovenia.

Science.gov (United States)

Pate, Mateja; Zajc, Urška; Kušar, Darja; Žele, Diana; Vengušt, Gorazd; Pirš, Tina; Ocepek, Matjaž

2016-02-01

Wildlife species are an important reservoir of mycobacterial infections that may jeopardise efforts to control and eradicate bovine tuberculosis (bTB), caused by Mycobacterium bovis. Slovenia is officially free of bTB, but no data on the presence of mycobacteria in wild animals has been reported. In this study, samples of liver and lymph nodes were examined from 306 apparently healthy free-range wild animals of 13 species in Slovenia belonging to the families Cervidae, Suidae, Canidae, Mustelidae and Bovidae. Mycobacteria were isolated from 36/306 (11.8%) animals (red deer, roe deer, fallow deer, wild boar and jackal) and identified by PCR, commercial diagnostic kits and sequencing. Non-tuberculous mycobacteria identified in five species were Mycobacterium peregrinum, M. avium subsp. hominissuis, M. intracellulare, M. confluentis, M. fortuitum, M. terrae, M. avium subsp. avium, M. celatum, M. engbaekii, M. neoaurum, M. nonchromogenicum and M. vaccae. Copyright © 2015 Elsevier Ltd. All rights reserved.

Distribución parcial de Paragonimus (Digenea: Troglotrematidae en Antioquia, por presencia de metacercarias en cangrejos dulciacuícolas

Directory of Open Access Journals (Sweden)

Luz Elena Velásquez

2008-12-01

Full Text Available Introducción. La paragonimosis, o distomatosis pulmonar, es una enfermedad con sintomatología similar a la observada en la tuberculosis. Es causada por parásitos del género Paragonimus (Digenea: Troglotrematidae. Las personas se infectan al consumir cangrejos crudos o mal cocidos, con metacercarias del parásito. El primer foco de paragonimosis humana en Colombia se registró durante 1995 en Urrao, Antioquia, donde se hallaron dos especies de cangrejos que hospedaban el parásito. En el 2005 se capturaron cangrejos con metacercarias de Paragonimus en Medellín, lo que motivó la búsqueda del parásito en otras localidades, mediante su presencia en estos crustáceos. Objetivo. Establecer la distribución de Paragonimus en Antioquia, evaluando la presencia de metacercarias en macrocrustáceos braquiuros, dulciacuícolas. Materiales y métodos. Desde 2005 hasta 2007 se capturaron cangrejos en 13 municipios antioqueños. Se relajaron y sacrificaron para la búsqueda del digeneo y la identificación taxonómica. Resultados. En nueve municipios se capturaron 52 cangrejos, 42 (80,76% con metacercarias de Paragonimus. Todos los crustáceos se determinaron como Pseudothelphusidae, de los géneros Hypolobocera y Strengeriana, y se asignaron a cuatro especies. Tres se registran por primera vez como huéspedes del parásito. Conclusión. Se inicia la construcción de un mapa con la distribución de Paragonimus en Antioquia que incluye por primera vez zonas urbanizadas. Se ratifican el consumo y la manipulación de los cangrejos crudos y mal cocidos como factores de riesgo para la infección humana. Se propone a los cangrejos como agentes focalizadores de paragonimosis por ser asequibles y de fácil diagnóstico.

Principais doenças de Anonáceas no Brasil: descrição e controle

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Nilton Tadeu Vilela Junqueira

2014-01-01

Full Text Available Várias doenças podem afetar folhas, caules, flores e frutos de gravioleira, pinheira e atemoia em diferentes estádios de seus desenvolvimentos. Geralmente, as doenças mais importantes são causadas por fungos durante o florescimento e a frutificação. Também podem ocorrer murchas ou podridões de raízes, causadas por nematoides e patógenos do solo. A antracnose (Colletotrichum gloeosporioides, murcha ou podridões de raízes (Rhizoctonia solani, Cylindrocladium clavatum, Phytophthora sp., Pythium sp., Phytophthora nicotianae var. parasitica, cancros (Albonectria rigidiuscula e podridão de frutos (Botryodiplodia theobromae, sin. Lasiodiplodia theobromae são as mais importantes. Por outro lado, a podridão-parda-do-fruto (Rhizopus stolonifer provoca perdas expressivas na produção de graviola. A seguir, são descritas as principais doenças que afetam estas espécies de anonáceas, seus agentes causais e as medidas de controle.

Drug susceptibility testing of Mycobacterium tuberculosis to fluoroquinolones

DEFF Research Database (Denmark)

Johansen, I S; Larsen, A R; Sandven, P

2003-01-01

In the first attempt to establish a quality assurance programme for susceptibility testing of Mycobacterium tuberculosis to fluoroquinolones, 20 strains with different fluoroquinolone susceptibility patterns were distributed by the Supranational Reference Laboratory in Stockholm to the other...

Diffuse Type Primary Mycobacterium Tuberculosis of the Breast: A Case Report

Energy Technology Data Exchange (ETDEWEB)

Kim, Hyun A; Kang, Bong Joo; Kim, Sung Hun; Kim, Hnana; Lee, Ah Won [Seoul St. Mary' s Hospital, The Catholic University of Korea, Seoul (Korea, Republic of)

2011-12-15

Tuberculous mastitis is a rare manifestation of mycobacterium tuberculosis infection. It mimics inflammatory breast cancer or other pyogenic inflammations. In most of the tuberculous mastitis reports, coexisting or prior tuberculosis infection and secondary infection of the breast by direct spread via axillary or cervical lymphadenopathy, or hematogenous spread have been noted. We describe the mammographic and ultrasonographic findings of a case of diffuse type mycobacterium tuberculosis of the breast showing diffuse edema which was confirmed as tuberculosis through biopsy and had no evidence of old or concurrent pulmonary tuberculosis on chest computed tomography

Mixed Cutaneous Infection Caused by Mycobacterium szulgai and Mycobacterium intermedium in a Healthy Adult Female: A Rare Case Report

Directory of Open Access Journals (Sweden)

Amresh Kumar Singh

2015-01-01

Full Text Available Nontuberculous mycobacteria (NTMs are ubiquitous and are being increasingly reported as human opportunistic infection. Cutaneous infection caused by mixed NTM is extremely rare. We encountered the case of a 46-year-old female, who presented with multiple discharging sinuses over the lower anterior abdominal wall (over a previous appendectomy scar for the past 2 years. Microscopy and culture of the pus discharge were done to isolate and identify the etiological agent. Finally, GenoType Mycobacterium CM/AS assay proved it to be a mixed infection caused by Mycobacterium szulgai and M. intermedium. The patient was advised a combination of rifampicin 600 mg once daily, ethambutol 600 mg once daily, and clarithromycin 500 mg twice daily to be taken along with periodic follow-up based upon clinical response as well as microbiological response. We emphasize that infections by NTM must be considered in the etiology of nonhealing wounds or sinuses, especially at postsurgical sites.

Mean effective sensitivity for Mycobacterium avium subsp paratuberculosis infection in cattle herds

DEFF Research Database (Denmark)

Kirkeby, Carsten; Græsbøll, Kaare; Hisham Beshara Halasa, Tariq

2015-01-01

Background: Mycobacterium avium subsp. paratuberculosis (MAP) infections in cattle are generally challenging to detect and cost-effective test strategies are consequently difficult to identify. MAP-specific antibody ELISAs for milk and serum are relatively inexpensive, but their utility is influe......Background: Mycobacterium avium subsp. paratuberculosis (MAP) infections in cattle are generally challenging to detect and cost-effective test strategies are consequently difficult to identify. MAP-specific antibody ELISAs for milk and serum are relatively inexpensive, but their utility...

Doença de Krabbe — A Propósito de Dois Casos Clínicos

OpenAIRE

Sampaio, Virgínia; Belo, Emília; Frutuoso, Simão; Cerqueira, Rui; Mendanha, Sérgio; Maciel, Idalina

2014-01-01

A doença de Krabbe também designada por leucodistrofia de células globoides é uma doença autossómica recessiva rara causada por um déficede um enzima, o galactosilceramidase. Os autores apresentam dois casos clínicos da forma de apresentação desta leucodistrofia no lactente.

Bloodstream Infections with Mycobacterium tuberculosis among HIV patients

Centers for Disease Control (CDC) Podcasts

This podcast looks at bloodstream infections with Mycobacterium tuberculosis and other pathogens among outpatients infected with HIV in Southeast Asia. CDC health scientist Kimberly McCarthy discusses the study and why bloodstream infections occur in HIV-infected populations.

Safety assessment in primary Mycobacterium tuberculosis smear ...

African Journals Online (AJOL)

Introduction Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is transmitted mainly through aerosolization of infected sputum which puts laboratory workers at risk in spite of the laboratory workersf risk of infection being at 3 to 9 times higher than the general public. Laboratory safety should therefore be ...

Mycobacterium tuberculosis and Mycobacterium marinum non-homologous end-joining proteins can function together to join DNA ends in Escherichia coli.

Science.gov (United States)

Wright, Douglas G; Castore, Reneau; Shi, Runhua; Mallick, Amrita; Ennis, Don G; Harrison, Lynn

2017-03-01

Mycobacterium tuberculosis and Mycobacterium smegmatis express a Ku protein and a DNA ligase D and are able to repair DNA double strand breaks (DSBs) by non-homologous end-joining (NHEJ). This pathway protects against DNA damage when bacteria are in stationary phase. Mycobacterium marinum is a member of this mycobacterium family and like M. tuberculosis is pathogenic. M. marinum lives in water, forms biofilms and infects fish and frogs. M. marinum is a biosafety level 2 (BSL2) organism as it can infect humans, although infections are limited to the skin. M. marinum is accepted as a model to study mycobacterial pathogenesis, as M. marinum and M. tuberculosis are genetically closely related and have similar mechanisms of survival and persistence inside macrophage. The aim of this study was to determine whether M. marinum could be used as a model to understand M. tuberculosis NHEJ repair. We identified and cloned the M. marinum genes encoding NHEJ proteins and generated E. coli strains that express the M. marinum Ku (Mm-Ku) and ligase D (Mm-Lig) individually or together (LHmKumLig strain) from expression vectors integrated at phage attachment sites in the genome. We demonstrated that Mm-Ku and Mm-Lig are both required to re-circularize Cla I-linearized plasmid DNA in E. coli. We compared repair of strain LHmKumLig with that of an E. coli strain (BWKuLig#2) expressing the M. tuberculosis Ku (Mt-Ku) and ligase D (Mt-Lig), and found that LHmKumLig performed 3.5 times more repair and repair was more accurate than BWKuLig#2. By expressing the Mm-Ku with the Mt-Lig, or the Mt-Ku with the Mm-Lig in E. coli, we have shown that the NHEJ proteins from M. marinum and M. tuberculosis can function together to join DNA DSBs. NHEJ repair is therefore conserved between the two species. Consequently, M. marinum is a good model to study NHEJ repair during mycobacterial pathogenesis. © The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen

DETERMINACIÓN DE LA SUSCEPTIBILIDAD A DROGAS DE PRIMERA LÍNEA EN AISLAMIENTOS DE MYCOBACTERIUM TUBERCULOSIS POR LA TÉCNICA DEL TUBO INDICADOR DE CRECIMIENTO MICOBACTERIANO

OpenAIRE

Claudia Rocío Sierra Parada; Édgar Alberto Sánchez Morales; Sandra Consuelo Henao Riveros; Alfredo Saavedra Rodríguez

2008-01-01

Antecedentes. La aparición de aislamientos de Mycobacterium tuberculosis resistentes a los medicamentos ha hecho que se busquen métodos más rápidos y confiables para la determinación de la susceptibilidad a las drogas antituberculosas. Objetivo. Determinación de la susceptibilidad a drogas antituberculosas de primera línea en aislamientos de M. tuberculosis utilizando el método del tubo indicador de crecimiento micobacteriano (MGIT). Material y métodos. Se estudiaron 49 aislamientos de...

Full genome sequence of a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007

DEFF Research Database (Denmark)

Afzal, Mamuna; Abidi, Soad; Mikkelsen, Heidi

We have sequenced a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007. The strain was isolated from faecal material of a 48 month old second parity Danish Holstein cow, with clinical symptoms of chronic diarrhoea and emaciation. The cultures were grown on Löwen......We have sequenced a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007. The strain was isolated from faecal material of a 48 month old second parity Danish Holstein cow, with clinical symptoms of chronic diarrhoea and emaciation. The cultures were grown......, consisting of 4317 unique gene families. Comparison with M. avium paratuberculosis strain K10 revealed only 3436 genes in common (~70%). We have used GenomeAtlases to show conserved (and unique) regions along the Ejlskov2007 chromosome, compared to 2 other Mycobacterium avium sequenced genomes. Pan......-genome analyses of the sequenced Mycobacterium genomes reveal a surprisingly open and diverse set of genes for this bacterial genera....

Ocorrência de mastite clínica e subclínica causada por Trichosporon beigelii em um rebanho leiteiro

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Cassiano Victoria

2006-04-01

Full Text Available Durante as atividades de extensão rural, desenvolvidas pelo Núcleo de Pesquisa em Mastites - NUPEMAS, do Departamento de Higiene Veterinária e Saúde Pública, da Faculdade de Medicina Veterinária e Zootecnia da Universidade Estadual Paulista - Botucatu - São Paulo - Brasil, no ano de 2002, foi isolado de uma propriedade leiteira do interior do estado, o fungo Trichosporon beigelii, em quatro animais com mastite clínica, e em três com mastite subclínica. Este fungo é de grande importância, pois é responsável pelo desenvolvimento de quadros de micose superficial, conhecidos como piedra branca. No Brasil, até o presente momento, não há relato de isolamento de Trichosporon beigelii, como agente causador de mastites. Este fato confirma a importância do exame microbiológico para o monitoramento das mastites, pois permite a identificação de patógenos tanto nas infecções intramamárias subclínicas como clínicas, evitando-se o tratamento com antimicrobianos, que nestes casos em particular, além de não exercer seu efeito terapêutico, pode predispor a infecções mistas por outros agentes fúngicos, na medida em que os antimicrobianos fornecem substrato para o desenvolvimento e manutenção destes.

Mortalidade em florestas de Pinus palustris causada por tempestade de raios

Science.gov (United States)

Kenneth W. Outcalt; Jorge Paladino Corrêa de Lima; Jose Américo de Mello Filho

2002-01-01

The importance of lightning as an ignition source for the fire driven Pinus palustris ecosystem is widely recognized. Lightning also impacts this system on a smaller scale by causing individual tree mortality. The objective of this study was to determine the level of mortality due to lightning activity at the Department of Energy's Savannah...

Mycobacterium tuberculosis monoarthritis in a child

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Rosenberg Alan M

2008-09-01

Full Text Available Abstract A child with isolated Mycobacterium tuberculosis monoarthritis, with features initially suggesting oligoarthritis subtype of juvenile idiopathic arthritis, is presented. This patient illustrates the need to consider the possibility of tuberculosis as the cause of oligoarthritis in high-risk pediatric populations even in the absence of a tuberculosis contact history and without evidence of overt pulmonary disease.

Febre crônica associada a abscesso esplênico causado por Staphylococcus epidermidis Chronic fever associated with splenic abscess due to Staphylococcus epidermidis

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Juliana Salles de Carvalho

2007-10-01

Full Text Available Abscessos ocultos são causa freqüente de febre crônica. Os abscessos esplênicos são entidades raras, usualmente associadas a quadros subjacentes de cirurgia abdominal, endocardite ou imunodepressão. Apresenta-se um caso de paciente com febre prolongada causada por um abscesso esplênico, cujo principal diagnóstico diferencial era leishmaniose visceral, que provavelmente esteve associado a traumatismo abdominal. O tratamento consistiu em antibioticoterapia seguida de esplenectomia.Occult abscesses are frequent causes of chronic fever. Splenic abscesses are rare entities that are usually associated with underlying conditions such as abdominal surgery, endocarditis or immunodepression. We report on the case of a patient with prolonged fever caused by a splenic abscess, whose main differential diagnosis was visceral leishmaniasis. However, this condition was probably related to abdominal trauma. The treatment consisted of antibiotics followed by splenectomy.

Mycobacterium avium subsp. paratuberculosis: presencia en los alimentos y su relación con la enfermedad de Crohn Mycobacterium avium subsp. paratuberculosis in food and its relationship with Crohn's disease

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K. Cirone

2007-03-01

Full Text Available La paratuberculosis o enfermedad de Johne es una enteritis crónica producida por Mycobacterium avium subsp. paratuberculosis, que afecta a bovinos y a otras especies. En la Argentina se ha caracterizado en rodeos bovinos y de ciervos, con aislamientos tipificados en distintos patrones genéticos. M. avium subsp. paratuberculosis ha sido vinculado en humanos con una inflamación crónica del intestino, denominada enfermedad de Crohn. Existen evidencias clínicas y experimentales que relacionan a M. avium subsp. paratuberculosis con la enfermedad en el humano, mediante su detección por PCR y por cultivo a partir de biopsias de órganos, de leche materna y de sangre de pacientes afectados. La leche y sus subproductos serían posibles fuentes de infección y se ha sugerido que M. avium subsp. paratuberculosis resistiría las condiciones de pasteurización. Diversos trabajos de investigación demostraron que esta micobacteria podría estar presente en leches comercializadas en diversos países, como Reino Unido, Estados Unidos, República Checa, y también en la Argentina. La presencia de M. avium subsp. paratuberculosis en productos lácteos y agua de consumo ha sido relacionada con la resistencia del microorganismo tanto a los procesos de elaboración como a los factores climáticos adversos, lo que enfatiza el rol de los alimentos y del agua como vías de transmisión al humano. Las investigaciones en curso podrían ratificar el riesgo y las implicancias de la exposición del humano a M. avium subsp. paratuberculosis a través de los alimentos y del agua contaminados, para determinar la importancia de la paratuberculosis como enfermedad zoonótica.Paratuberculosis or Johne's disease is a chronic enteritis of the cattle and other small ruminant animals caused by Mycobacterium avium subsp. paratuberculosis. In Argentina, the strains were characterized in beef and dairy cattle and deer in different genetic patterns by molecular tools. M. avium

Identification and characterization of the ESAT-6 homologue of Mycobacterium leprae and T-cell cross-reactivity with Mycobacterium tuberculosis

NARCIS (Netherlands)

Geluk, Annemieke; van Meijgaarden, Krista E.; Franken, Kees L. M. C.; Subronto, Yanri W.; Wieles, Brigitte; Arend, Sandra M.; Sampaio, Elizabeth P.; de Boer, Tjitske; Faber, William R.; Naafs, Ben; Ottenhoff, Tom H. M.

2002-01-01

In this paper we describe identification and characterization of Mycobacterium leprae ESAT-6 (L-ESAT-6), the homologue of M. tuberculosis ESAT-6 (T-ESAT-6). T-ESAT-6 is expressed by all pathogenic strains belonging to the M. tuberculosis complex but is absent from virtually all other mycobacterial

Validação da proteína MDP2 de Mycobacterium tuberculosis H37Rv como uma proteína intrinsecamente desordenada e geração de uma cepa nocaute para o gene hns

OpenAIRE

Abbadi, Bruno Lopes

2014-01-01

A proteína micobacteriana ligadora de DNA 2 (MDP2) de Mycobacterium tuberculosis é uma proteína pequena e de caráter básico, conhecida por se ligar ao DNA de uma forma não es-pecífica e de ancorar o nucleóide à membrana plasmática promovendo o seu desempacota-mento. Motivados por uma predição de desordem intrínseca in silico prévia, nós usamos um conjunto de técnicas complementares para caracterizar esta proteína, tais como determina-ção da estabilidade ao calor e a desnaturantes químicos, ge...

Mycobacterium bovis Infection of Red Fox, France.

Science.gov (United States)

Michelet, Lorraine; De Cruz, Krystel; Hénault, Sylvie; Tambosco, Jennifer; Richomme, Céline; Réveillaud, Édouard; Gares, Hélène; Moyen, Jean-Louis; Boschiroli, María Laura

2018-06-01

Mycobacterium bovis infection in wild red foxes was found in southern France, where livestock and other wildlife species are infected. Foxes frequently interact with cattle but have been underestimated as a reservoir of M. bovis. Our results suggest a possible role of the red fox in the epidemiology of bovine tuberculosis.

Adaptation and evolution of drug-resistant Mycobacterium tuberculosis

NARCIS (Netherlands)

Bergval, I.L.

2013-01-01

Many studies have been conducted on drug resistance and the evolution of Mycobacterium tuberculosis. Notwithstanding, many molecular mechanisms facilitating the emergence, adaptation and spread of drug-resistant tuberculosis have yet to be discovered. This thesis reports studies of the adaptive

Treinamento resistido controla a pressão arterial de ratos hipertensos induzidos por l-NAME

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Ayslan Jorge Santos de Araujo

2013-04-01

Full Text Available FUNDAMENTO: A hipertensão arterial é uma síndrome multifatorial, crônica, causada tanto por fatores congênitos ou adquiridos. OBJETIVO: Avaliar os efeitos do treinamento físico resistido (TR sobre pressão arterial, reatividade e morfologia vascular de ratos hipertensos induzidos por L-NAME. MÉTODOS: Ratos Wistar machos (200-250 g foram divididos em 3 grupos: normotenso sedentário (NS, hipertenso sedentário (HS e hipertenso treinado (HT. A hipertensão foi induzida pela administração de L-NAME (40 mg/kg na água de beber por 4 semanas. A pressão arterial foi avaliada antes e após o TR. O TR foi realizado utilizando 50% de 1RM, em 3 séries de 10 repetições, 3 vezes por semana, durante quatro semanas. A reatividade vascular foi mensurada em artéria mesentérica superior por curvas concentração resposta ao nitroprussiato de sódio (NPS e fenilefrina (FEN. Além disso, foram realizadas análises histológicas e estereológicas. RESULTADOS: O TR inibiu o aumento das pressões arteriais média e diastólica. Foi observada uma redução significativa na resposta máxima e na potência da FEN entre os grupos HS e HT. A análise histológica evidenciou aspecto normal para as túnicas íntima, média e adventícia em todos os grupos. Não houve diferença significativa nas áreas do lúmen, da túnica média e total das artérias dos grupos HS e HT em relação ao NS. A razão parede/lúmen arterial do grupo HS apresentou diferença significativa em relação ao NS (p < 0,05, mas esta não foi diferente do HT. CONCLUSÕES: O TR foi capaz de prevenir a elevação da pressão arterial sob as condições deste estudo. Este controle parece envolver a regulação de mecanismo vasoconstritor e a manutenção do diâmetro luminal de ratos hipertensos induzidos por L-NAME.

Dry-heat inactivation of "Mycobacterium canettii".

Science.gov (United States)

Aboubaker Osman, Djaltou; Garnotel, Eric; Drancourt, Michel

2017-06-09

"Mycobacterium canettii" is responsible for non-transmissible lymph node and pulmonary tuberculosis in persons exposed in the Horn of Africa. In the absence of direct human transmission, contaminated water and foodstuffs could be sources of contamination. We investigated the dry-heat inactivation of "M. canettii" alone and mixed into mock-infected foodstuffs by inoculating agar cylinders and milk with 10 4 colony-forming units of "M. canettii" CIPT140010059 and two "M. canettii" clinical strains with Mycobacterium tuberculosis H37Rv as a control. Exposed to 35 °C, M. tuberculosis H37Rv, "M canettii" CIPT140010059 and "M. canettii" 157 exhibited a survival rate of 108, 95 and 81%, which is significantly higher than that of "M. canettii" 173. However, all tested mycobacteria tolerated a 90-min exposure at 45 °C. In the foodstuff models set at 70 °C, no growing mycobacteria were visualized. This study supports the premise that "M. canettii" may survive up to 45 °C; and suggests that contaminated raw drinks and foodstuffs but not cooked ones may be sources of infection for populations.

Images of mycobacterium for nuclear reactions

International Nuclear Information System (INIS)

Lima, C.T.S.; Crispim, V.R.; Silva, M.G.

2007-01-01

According to the World Health Organization (WHO) tuberculosis is responsible for 2.9 million deaths annually worldwide. The necessity for optimizing time to detect the tuberculosis bacillus (mycobacterium tuberculosis) in the sputum samples of affected individuals (TB patients) led to the development of a methodology based on the doping with boron of the bacillus, submission of the samples to thermal neutron beam and ionizing particles, generating nuclear reactions of the types: 10 B (n,α) 7 Li and 10 B(α, p) 13 C. Images of these bacilli are obtained by means of the nuclear tracks produced in the CR-39 detector for particles products of these nuclear reactions, α and p. When the CR-39 is submitted to a chemical attack the traces are developed and the images of the microorganisms registered in the detector can be observed with a conventional light microscope, characterizing them by morphology. The use of this methodology results in images of the mycobacterium tuberculosis becoming more defined and enlarged than those obtained by bacilloscopy, in which the sample is submitted to the method of coloration of Ziehl-Neelsen (ZN) and observed in light microscopy. (author)

INFECTIOUS KERATOCONJUNTIVITIS OUTBREAK IN OVINES DUE TO Moraxella spp IN STATE OF GOIÁS, BRAZIL SURTO DE CERATOCONJUNTIVITE INFECCIOSA EM OVINOS CAUSADA POR Moraxella spp. NO ESTADO DE GOIÁS, BRASIL

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Nilo Sérgio Troncoso Chaves

2008-04-01

','serif'">Moraxella spp foi feito por meio de cultura nos Laboratórios de Microbiologia do Centro de Pesquisa de Alimentos da Escola de Veterinária da Universidade Federal de Goiás (APA/EV-UFG e do Departamento de Medicina Veterinária da Escola de Veterinária da Universidade Federal de Goiás (DMV/EV-UFG. O controle do foco na propriedade envolveu os protocolos: 1. higiênico, com o isolamento em ambiente de pouca luminosidade, repouso e alimentação adequada;  2. medicamentoso, mediante o uso de solução fisiológica 0,9% para a lavagem dos olhos, pomada oftálmica de cloridrato de oxitetraciclina para uso local e cloridrato de oxitetraciclina LA injetável, tanto para os animais doentes como para o restante do rebanho;  3. profilático, por meio de vacina comercial contra a ceratoconjuntive infeciosa e combate às moscas. A recuperação da maioria dos animais minimizou as perdas econômicas e evitou a disseminação da enfermidade na propriedade e para outras fazendas. No Brasil, existem poucos estudos a respeito de surtos de CCI em ovinos, o que motivou a realização deste trabalho, diante da escassez de informações sobre essa doença ocular, causada pela Moraxella spp, em ovinos, no estado de Goiás, Brasil

JST Thesaurus Headwords and Synonyms: Mycobacterium tuberculosis [MeCab user dictionary for science technology term[Archive

Lifescience Database Archive (English)

Full Text Available MeCab user dictionary for science technology term Mycobacterium tuberculosis 名詞 一般 * * * * 結核...菌 ケッカクキン ケッカクキン Thesaurus2015 200906007893342100 C LS07 UNKNOWN_2 Mycobacterium tuberculosis

Assessing the effectiveness of low-pressure ultraviolet light for inactivating Mycobacterium avium complex (MAC) micro-organisms

Science.gov (United States)

Aims: To assess low-pressure ultraviolet light (LP-UV) inactivation kinetics of Mycobacterium avium complex (MAC) strains in a water matrix using collimated beam apparatus. Methods and Results: Strains of M. avium (n = 3) and Mycobacterium intracellulare (n = 2) were exposed t...

Mycobacterium minnesotense sp. nov., a photochromogenic bacterium isolated from sphagnum peat bogs.

Science.gov (United States)

Hannigan, Geoffrey D; Krivogorsky, Bogdana; Fordice, Daniel; Welch, Jacqueline B; Dahl, John L

2013-01-01

Several intermediate-growing, photochromogenic bacteria were isolated from sphagnum peat bogs in northern Minnesota, USA. Acid-fast staining and 16S rRNA gene sequence analysis placed these environmental isolates in the genus Mycobacterium, and colony morphologies and PCR restriction analysis patterns of the isolates were similar. Partial sequences of hsp65 and dnaJ1 from these isolates showed that Mycobacterium arupense ATCC BAA-1242(T) was the closest mycobacterial relative, and common biochemical characteristics and antibiotic susceptibilities existed between the isolates and M. arupense ATCC BAA-1242(T). However, compared to nonchromogenic M. arupense ATCC BAA-1242(T), the environmental isolates were photochromogenic, had a different mycolic acid profile and had reduced cell-surface hydrophobicity in liquid culture. The data reported here support the conclusion that the isolates are representatives of a novel mycobacterial species, for which the name Mycobacterium minnesotense sp. nov. is proposed. The type strain is DL49(T) (=DSM 45633(T) = JCM 17932(T) = NCCB 100399(T)).

Bioaktivitas Ekstrak Metanol Daun Pegagan (Centella Asiatica L. Terhadap Pertumbuhan Bakteri Mycobacterium Tuberculosis

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Yusran Yusran

2016-01-01

Full Text Available Plants gotu kola (Centella Asiatica L .Urban is a wild plant that efficacious as remedies traditional cure disease tuberculosis (TB.TB is disease contagious infection caused by bacteria mycobacterium tuberculosis. Research aims to understand the ability extract methanol leaves gotu kola red and leaves gotu kola green and determines the concentration optimal extract methanol leaves gotu kola red and leaves gotu kola green and to know the comparison between extract methanol leaves gotu kola red with an extract methanol leaves gotu kola green in inhibits the activity of mycobacterium tuberculosis.Extraction done with the methods maceration use methanol and continued with evaporation until obtained extract viscous .Testing antibacterial activity done in a microscopic observation drug susceptibility ( mods use plate petri dish 24 hole with the variation of concentration ie 20%,40%, 60%, 80% and 100%.The results of testing show that extracts methanol leaves gotu kola red and leaves gotu kola green positive capable of inhibiting the growth of bacteria mycobacterium tuberculosis with inhibition optimal in concentration 80 % and 100 % characterized by the absence of growth bacteria colonies which are (- or 0 %.Extract methanol leaves gotu kola green capable of inhibiting the growth of bacteria mycobacterium tuberculosis better than extract methanol leaves gotu kola red seen in concentration 40% and 60%.

Bovine paratuberculosis: a review of the advantages and disadvantages of different diagnostic tests Paratuberculosis bovina: una revisión sobre las ventajas y desventajas de las diferentes pruebas diagnósticas

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Liliana R. Gilardoni

2012-09-01

Full Text Available Paratuberculosis (PTB, or Johne's disease, is a chronic infectious granulomatous enteritis of ruminants, caused by Mycobacterium avium subspecies paratuberculosis (Map. It is characterized by diarrhea and progressive cachexia, which may cause the death of the animal. Calves are the most susceptible to infection. Infected animals excrete Map mainly by the feces. PTB is endemic worldwide, with high prevalence levels, strong economic impact and public health relevance because of its possible association with Crohn's disease. Although the current reference diagnostic test is identification of Map in the bacterial culture, there are different diagnostic tests to identify infected individuals and/or herds. The sensitivity and specificity of these tests vary according to the stage of the disease in the animals to be evaluated. The correct choice and application of each of these diagnostic tests will ensure their success and may allow to establish a control program. The aim of this work is to review and discuss the different diagnostic tests used in the detection of Map-infected animals, focusing on their advantages and disadvantages.La paratuberculosis (PTBC o enfermedad de Johne es una enteritis granulomatosa crónica de rumiantes, causada por Mycobacterium avium subsp. paratuberculosis (Map. Se caracteriza por producir diarrea y caquexia progresiva, la cual conduce a la muerte del animal. Los terneros son los animales más proclives a la infección. Los animales infectados excretan Map, principalmente por las heces. La PTBC es una enfermedad endémica a nivel mundial, con altos niveles de prevalencia, fuerte impacto económico e importancia en salud pública, debido a su posible asociación con la enfermedad de Crohn. Aunque la prueba de referencia diagnóstica es la identificación de Map en cultivo bacteriológico, existen diferentes pruebas diagnósticas para detectar animales o rodeos infectados. La sensibilidad y especificidad de estas pruebas

Molecular Characterization of the Resistance of Mycobacterium ...

African Journals Online (AJOL)

Abstract. Purpose: To characterize the resistance of Mycobacterium tuberculosis to second line drugs using a line probe assay. Methods: ... Marne-la-Coquette,. France). Bacterial isolates contained in 500 µl of liquid culture were heat- inactivated at 95 °C for 30 min and then sonicated for 12 min. Finally, the suspension was ...

Manifestaciones mucocutáneas atípicas por fiebre por el virus del chikungunya en neonatos y lactantes de Cúcuta, Los Patios y Villa del Rosario, Norte de Santander, Colombia, 2014

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Claudia Marcela Muñoz

2016-09-01

Full Text Available Introducción. Se han observado manifestaciones clínicas atípicas en recién nacidos y lactantes con fiebre por el virus del chikungunya. Objetivo. Describir los casos de fiebre causada por el virus del chikungunya en recién nacidos y lactantes con lesiones mucocutáneas atípicas. Materiales y métodos. Se revisaron las historias clínicas y los resultados de laboratorio y de patología en tres hospitales regionales de los casos de recién nacidos y lactantes diagnosticados con el virus del chikungunya que presentaban lesiones mucocutáneas atípicas; se hizo una búsqueda activa comunitaria en los barrios de residencia de los pacientes. Resultados. De 18 casos sospechosos de chikungunya en recién nacidos y lactantes, 11 pacientes con diagnóstico confirmado presentaron manifestaciones mucocutáneas atípicas. Seis de los 11 casos confirmados eran niños menores de cinco meses de edad. Los síntomas más frecuentes fueron: fiebre, eritema, irritabilidad y diarrea. Tres de los pacientes estaban infectados con dengue y chikungunya. Las úlceras se presentaron en cuero cabelludo, abdomen, región genital y perianal. En la búsqueda activa comunitaria se encontraron altas tasas de ataque de la enfermedad. Conclusiones. Se presenta un informe de las manifestaciones mucocutáneas en recién nacidos y lactantes diagnosticados con fiebre por el virus del chikungunya en Colombia. El rápido desarrollo de las úlceras se debe, probablemente, a la reacción inmunitaria al virus. Es necesario priorizar la atención de las mujeres embarazadas que presenten síntomas una semana antes del parto y hacerle seguimiento a los recién nacidos, para hacer el diagnóstico temprano del chikungunya y evitar complicaciones.

Efecto del acibenzolar-s-metil sobre el desarrollo de la virosis causada por potyvirus en tomate de árbol

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Mejía A. Diana Marcela

2009-04-01

Full Text Available

El principal limitante del cultivo de tomate de árbol en Colombia es el ataque de virus, el cual reduce la producción y calidad de la fruta. En condiciones de invernadero se evaluó el efecto del inductor de resistencia Acibenzolar-S-metil- ASM (Boost® sobre la incidencia y severidad de la virosis en plantas inoculadas con extracto de hojas naturalmente infectadas con virus, mediante aplicación foliar de 0,08% de producto comercial por planta cada 20 días. La incidencia de la virosis se redujo en 50% cuando el inductor se aplicó antes de la inoculación con el virus, seguido de dos aplicaciones adicionales de ASM, comparado con 100% de incidencia en las plantas inoculadas solo con el extracto de plantas infectadas con el virus, tendencia encontrada también en la curva de desarrollo de la enfermedad. Se observó un retraso de 7 días en la aparición de síntomas cuando se aplicó el inductor antes de la inoculación así como se observó una reducción significativa en el grado de severidad de la enfermedad. El potyvirus fue detectado mediante la prueba ELISA; sin embargo, no se encontraron diferencias estadísticas significativas en su concentración entre los diferentes tratamientos.

First report of Mycobacterium canariasense catheter-related bacteremia in the Americas.

Science.gov (United States)

Paniz-Mondolfi, Alberto; Ladutko, Lynn; Brown-Elliott, Barbara A; Vasireddy, Ravikiran; Vasireddy, Sruthi; Wallace, Richard J; Jakubiec, Wesley; Brecher, Stephen; Campbell, Sheldon

2014-06-01

Mycobacterium canariasense is a recently described late-pigmenting, rapidly growing mycobacterium linked to bacteremia in patients with underlying malignant diseases. We report a case of M. canariasense infection in a patient from Massachusetts with underlying diffuse B cell lymphoma, which was identified both by multilocus sequence typing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). To our knowledge, this is the first description after its original identification in Spain and the first report of this opportunistic pathogen in the Americas. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Actividad antimicobacteriana de terpenos Antimycobacterial activity of terpenes

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Juan Gabriel Bueno-Sánchez

2009-12-01

Full Text Available Introducción: La tuberculosis (TB, causada por Mycobacterium tuberculosis es la mayor causa de mortalidad mundial por un único agente patógeno. Asimismo, un gran número de micobacterias no tuberculosas, especialmente M. avium, M. intracellulare y M. chelonae, causan infecciones oportunistas en pacientes con SIDA. Muchos terpenos poseen actividad biológica y se emplean en el tratamiento de diversas enfermedades, razón que los hace fuente de moléculas promisorias. Objetivo: El objetivo del presente estudio fue determinar la actividad antimicobacteriana de 16 terpenos contra M. tuberculosis H37Rv y un aislamiento clínico de M. chelonae. Materiales y métodos: Se obtuvo la concentración mínima inhibitoria (CMI de los mismos y se realizaron curvas de letalidad para establecer actividad bactericida, empleando una técnica de macrodilución en caldo estandarizada para este tipo de compuestos volátiles. Resultados: Los terpenos con menor valor de CMI fueron timol y carvacrol, con concentraciones de 125-250 μg/mL, y actividad bactericida contra los dos microorganismos. Geraniol, mirceno, ρ-cimeno, alfa-pineno, presentaron valores de CMI entre 250 y 500 μg/mL. Conclusiones: Algunos terpenos han presentado actividad importante contra microorganismos del género Mycobacterium, sin embargo los valores de CMI obtenidos no explican el efecto antimicrobiano presentado por el aceite completo, se requiere evaluar las interacciones de sinergismo y/o antagonismo entre los terpenos para determinar los componentes responsables de la acción farmacológica. Salud UIS 2009; 41: 231-235Introduction: Tuberculosis (TB caused by Mycobacterium tuberculosis is the major source of global mortality from a single pathogen. Moreover, a large number of nontuberculous mycobacteria, especially M. avium, M. intracellulare and M. chelonae, cause opportunistic infection in AIDS patients. Terpenes, possess a wide spectrum of biological activity and are used in the

Papel del calcio en la modulación de la supervivencia intracelular de Mycobacterium tuberculosis

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Luis Fernando García

2001-04-01

Full Text Available

Mycobacterium tuberculosis ( Mtb induce alteraciones en la señalización que podrían favorecer el crecimiento intracelular de la bacteria. Hay alteraciones en respuestas al IFNγ, activación de fosfatasas, inhibición del fagolisosoma y cambios en los flujos
de iones como el Ca2+. Estos eventos dependen de los receptores por los cuales sea reconocida la micobacteria, por ejemplo, en macrófagos murinos la interacción con el receptor de manosa (MMR, pero no con CD14, ni receptores tipo “scavenger” induce
flujos de Ca2+, que alteran la mitocondria y se asocian con apoptosis. En macrófagos humanos, la fagocitosis a través de receptores Fc, pero no de receptores de complemento, aumenta el Ca2+ que favorece la translocación al fagosoma de la calmodulina (CaM que activa la kinasa II dependiente de CaM (CaMKII, permitiendo la fusión del fagolisosoma y disminuyendo el crecimiento de Mtb. El bloqueo de esta vía favorece el crecimiento de la micobacteria. Esto indica que el Ca2+ puede modular la apoptosis y las señales que controlan la micobacteria, sugiriendo que la modulación de esta vía puede ser un blanco putativo para la intervención farmacológica. Por lo tanto proponemos como objetivo general establecer si la terapia anti-micobacteriana modula las vías de señalización dependientes de Ca2+ y la actividad de la CaMKII en macrófagos infectados con Mtb.

INSATISFACCIÓN POR LA IMAGEN CORPORAL Y LA BAJA AUTOESTIMA POR LA APARIENCIA FÍSICA EN ESTUDIANTES DE LA FACULTAD DE MEDICINA DE LA UNIVERSIDAD DE LOS ANDES DEL ESTADO MERIDA VENEZUELA

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Pedro López Atencio

2008-07-01

Full Text Available La presente investigación tuvo como propósito central evaluar la insatisfacción por la imagen corporal y su relación con la baja autoestima por la apariencia física en estudiantes de la Facultad de Medicina de la Universidad de Los Andes en Mérida Venezuela. El estudio fue de tipo no experimental correlacional. La muestra estuvo constituida por 189 estudiantes, 27% masculino y 73% femenino, con una edad promedio de 19,58 años ± 1,57 (varones 19,81 años ± 1,74 y para las mujeres 20,24 años ± 1,76. Los participantes fueron seleccionados de los cursos del primer año de las carreras de: medicina, enfermería y nutrición, los cuales fueron seleccionados de forma intencional. El instrumento empleado para determinar la Insatisfacción por la Imagen Corporal fue el Body Shape Questionnaire (Cooper y Taylor, 1987. El método de Graffar Modificado fue utilizado para determinar el estrato socioeconómico de los participantes (Méndez y De Méndez, 1994. A los datos se les aplicó el análisis descriptivo (frecuencia, porcentajes, media e inferencial (ANOVA de un solo factor a través del paquete estadístico SPSS versión 9.0. Entre los principales hallazgos en el estudio se pudo determinar una asociación estadísticamente significativa entre la insatisfacción con la imagen corporal, la baja autoestima por la apariencia física con el género χ2 (2, N= 189 = 9,686, p=0,008. A través de ANOVA se pudo determinar que las diferencias en las medias para los niveles de insatisfacción y baja autoestima con la imagen corporal y el género son estadísticamente significativas, F=11,236; p=0,008, F=10,23; p=0,002 respectivamente. Conclusiones: los resultados obtenidos permiten sugerir sobre la relación que existe entre la insatisfacción y la baja autoestima por la apariencia física, existiendo un rechazo por la propia imagen corporal causada por la percepción distorsionada o no de la misma, lo que podría afectar la autoestima por la

Factores asociados a los accidentes por exposición percutánea en personal de enfermería en un hospital de tercer nivel

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Gallardo López Mª Teresa

1997-01-01

Full Text Available FUNDAMENTO: Los accidentes por exposición percutánea suponen aproximadamente un tercio de los accidentes laborales del personal sanitario hospitalario. Su importancia estriba en las enfermedades causadas por patógenos transmisibles por esta vía (hepatitis B, hepatitis C, infección VIH. Objetivos: describir los accidentes de este tipo notificados en un hospital de tercer nivel; identificar factores asociados a estos accidentes en personal de enfermería; construir un modelo predictivo del riesgo individual de accidentarse. MÉTODOS: Estudio descriptivo de una cohorte retrospectiva compuesta por todas las personas que notificaron un accidente entre el 1-1-93 y el 30-6-96. Estudio de casos y controles en el personal de enfermería durante el período 1-1-95 al 30-6-96, analizado mediante regresión logística múltiple. RESULTADOS: La incidencia acumulada de accidentes en un año fue de 0,078 para las/-os enfermeras/-os. En el 57,3% de los casos estuvieron implicadas agujas de jeringas desechables o precargadas. La incidencia acumulada en un año fue mayor para las agujas de cateterismo intravenoso (8,5 por 100.000. El riesgo de accidentabilidad, ajustado por variables confundentes, fue mayor para las/-os enfermeras/-os (OR=3,22; I.C.95%=1,96-5,27, para los trabajadores de la Unidad de Hemodiálisis (OR=35,21; I.C.95%=3,74-331,16 y para aquéllos con contrato eventual (OR=4,50; I.C.95%=2,24-9,04. CONCLUSIONES: Los accidentes por exposición percutánea en este hospital son más frecuentes entre el personal de enfermería y se producen, fundamentalmente, con algún tipo de aguja hueca. Se han identificado factores asociados a estos accidentes, lo que permite dirigir programas preventivos específicos sobre trabajadores con más riesgo. El modelo obtenido es válido para estimar el grado de accidentabilidad individual en los sujetos estudiados.

Efecto del estrés por sequía en el desarrollo de la antracnosis en tres ecotipos de Stylosanthes spp.

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Nuñez Luis E.

1986-09-01

Full Text Available En condiciones de invernadero, se evaluó el efecto del estrés por segura sobre la incidencia y severidad de antracnosis, causada por C. gloesporioides en S. guianensis var. guianensis CIAT 136, var pauciflora CIAT 1283 y S. capitata CIAT 1315; al mismo tiempo se estudió las diferentes respuestas morfológicas y fisiológicas de los tres ecotipos bajo déficit de agua en el suelo y presión del patógeno. Los ecotipos de Stylosanthes estudiados no solo toleran la deshidratación (- 26 bares sino también exhiben importantes mecanismos de adaptación morfológica que tienden a evitarla. EI efecto aditivo del estrés causado por antracnosis en condiciones de sequía, afectó la persistencia de los ecotipos de Stylosanthes spp. más susceptibles.

Under glasshouse conditiones, the effect of drought stress on the incidence and severity of antracnosis, caused by C. gloeosporioides, in S. guianensis var. guianensis CIAT 136, var. pauciflora CIAT 1283 and in S. capitata CIAT 1315 was evaluated; at the same time, the different morphological and physiological response of the three ecotypes under deficiency of water in the soil and pathogen pressure were studied. The ecotypes of Stylosanthes studies not only tolerate dehydration (- 26 bares but also shoved important mechanisms of morphological adaptation that they have to avoid it. The additive effect of stress caused by antracnose in drought conditions affected the persistence of more susceptible ecotypes.

Obtención y caracterización parcial de un extracto lipídico de la membrana externa de Mycobacterium smegmatis

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Nadine Alvarez

2009-04-01

Full Text Available En la actualidad, los antígenos lipídicos de las micobacterias constituyen blancos atractivos para el desarrollo de nuevas formulaciones vacunales contra la tuberculosis. En nuestro trabajo se realizó la caracterización parcial de un extracto lipídico de pared celular de Mycobacterium smegmatis mediante cromatografía de capa delgada y Dot blot frente a gammaglobulina humana. Se identificó, fundamentalmente, la presencia de fosfolípidos y ácidos micólicos en el extracto lipídico y se observó un elevado reconocimiento de los mismos por la gammaglobulina humana, lo cual indica la importancia de continuar los estudios de inmunoprotección empleando antígenos lipídicos de micobacterias.

Resistencia al desgaste de recubrimientos Fe-Nb-Cr-W, Nb, AISI 1020 y AISI 420 producidos por proyección térmica por arco eléctrico

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López-Covaleda, E. A.

2013-10-01

propiedades mecánicas como la dureza. Por ejemplo, los recubrimientos más blandos tienen las mayores tasas de desgaste y su modo fallo se caracterizó por deformación plástica causada por indentación del abrasivo; por su parte los materiales más duros presentan la mejor resistencia al desgaste y un modo de fallo por microarado. Los detalles de los mecanismos de desgaste de los recubrimientos producidos se describen en esta investigación.

Una prueba de captura rápida de antígenos con tiras reactivas para el diagnóstico de malaria por P. falciparum

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1997-01-01

Full Text Available Los avances recientes en el diagnóstico de infecciones causadas por Plasmodium falciparum han permitido considerar la posibilidad de complementar la microscopia óptica con una prueba estandarizada de captura de antígenos con tiras reactivas basada en la detección de una proteína específica del parásito, que es segregada por los estadios sanguíneos asexuados y los gametocitos inmaduros, pero no por otros estadios. Los ensayos de campo indican que esta prueba proporciona resultados replicables con un umbral de detección de parasitemia de P. falciparum similar al obtenido con microscopia habitual de alta calidad para malaria y una especificidad y sensibilidad de alrededor de 90% en comparación con la microscopia habitual con extensión de sangre en capa gruesa. La estabilidad, reproducibilidad y facilidad de uso de la prueba indican claramente sus posibilidades de aplicación en el tratamiento de la malaria, particularmente en el nivel de atención de salud periférico, siempre y cuando se pueda garantizar su precisión y su costo sea módico. También debe considerarse la posibilidad de usarla más ampliamente donde lo justifiquen los requisitos operativos y los recursos y donde las decisiones se basen en una evaluación adecuada de los sistemas de prestación de asistencia de salud existentes.

Co-infecção por Mycobacterium tuberculosis e vírus da imunodeficiência humana: uma análise epidemiológica em Taubaté (SP Co-infection with Mycobacterium tuberculosis and human immunodeficiency virus: an epidemiological analysis in the city of Taubaté, Brazil

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Luiz Gustavo Miranda de Carvalho

2006-10-01

Full Text Available OBJETIVO: Discutir os principais aspectos da co-infecção por Mycobacterium tuberculosis e vírus da imunodeficiência humana no município de Taubaté (SP nos anos de 2001 e 2002. MÉTODOS: Este trabalho apresenta o levantamento epidemiológico dos casos de tuberculose ocorridos em Taubaté em 2001 e 2002. RESULTADOS: Foram analisados 250 casos de tuberculose, dos quais 70 corresponderam a casos de sorologia positiva para o vírus da imunodeficiência humana (28%, 95 de sorologia negativa (38%, e para 85 pacientes a sorologia não foi realizada (34%. Com relação ao primeiro grupo houve predomínio do sexo masculino e da faixa etária de 30 a 40 anos, a forma clínica de tuberculose mais comum foi a pulmonar (65,71% e a taxa de cura foi de 59,38% . No grupo de pacientes para os quais não se comprovou a co-infecção também predominou o sexo masculino e ocorrência na mesma faixa etária, sendo a forma clínica mais comum também a pulmonar (70,55% e a taxa de cura foi de 81,63%. CONCLUSÃO: Concluiu-se que o vírus da imunodeficiência humana é importante na epidemiologia da tuberculose e, portanto, o teste sorológico para o vírus da imunodeficiência humana deve ser realizado quando se diagnostica a presença de tuberculose.OBJECTIVE: To discuss the main aspects of co-infection with Mycobacterium tuberculosis and human immunodeficiency virus in the city of Taubaté (located in the state of São Paulo, Brazil in 2001 and 2002. METHODS: This study presents epidemiological data on tuberculosis cases occurring in Taubaté in 2001 and 2002. RESULTS: Of the 250 cases of tuberculosis analyzed, 70 (28% presented human immunodeficiency virus seropositivity, 95 (38% presented human immunodeficiency virus seronegativity, and 85 (34% were patients who had not been submitted to serological testing. In the first group (tuberculosis and human immunodeficiency virus seropositivity, males from 30 to 40 years of age predominated, the most common clinical

Siderocalin inhibits the intracellular replication of Mycobacterium tuberculosis in macrophages

DEFF Research Database (Denmark)

Johnson, Erin E; Srikanth, Chittur V; Sandgren, Andreas

2010-01-01

Siderocalin is a secreted protein that binds to siderophores to prevent bacterial iron acquisition. While it has been shown to inhibit the growth of Mycobacterium tuberculosis (M.tb) in extracellular cultures, its effect on this pathogen within macrophages is not clear. Here, we show that sideroc......Siderocalin is a secreted protein that binds to siderophores to prevent bacterial iron acquisition. While it has been shown to inhibit the growth of Mycobacterium tuberculosis (M.tb) in extracellular cultures, its effect on this pathogen within macrophages is not clear. Here, we show...... findings are consistent with an important role for siderocalin in protection against M.tb infection and suggest that exogenously administered siderocalin may have therapeutic applications in tuberculosis....

Methanol production by Mycobacterium smegmatis

International Nuclear Information System (INIS)

Weisman, L.S.; Ballou, C.E.

1988-01-01

Mycobacterium smegmatis cells produce [ 3 H]methanol when incubated with [methyl- 3 H]methionine. The methanol is derived from S-adenosylmethionine rather than methyltetrahydrofolate. M. smegmatis cells carboxymethylate several proteins, and some of the methanol probably results from their demethylation, but most of the methanol may come from an unidentified component with a high gel mobility. Although methanol in the medium reached 19 μM, it was not incorporated into the methylated mannose polysaccharide, a lipid carrier in this organism

Tuberkulose forårsaget af Mycobacterium africanum

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Bek, Dorte; Kjeldsen, Marianne Kirstine; Hansen, Nikolaj Friis

2010-01-01

Tuberkulose (TB) forårsages af patogene arter fra Mycobacterium tuberculosis komplekset (MTBC) og har en incidens på cirka 7/100.000 i Danmark. På mistanke om TB hos en akut indlagt 40 årig afrikansk mand initieredes anti-TB behandling. Efter 13 timers indlæggelse afgik patienten ved døden. Fra...

rBCG30-induced immunity and cross-protection against Mycobacterium leprae challenge are enhanced by boosting with the Mycobacterium tuberculosis 30-kilodalton antigen 85B.

Science.gov (United States)

Gillis, Thomas P; Tullius, Michael V; Horwitz, Marcus A

2014-09-01

Leprosy remains a major global health problem and typically occurs in regions in which tuberculosis is endemic. Vaccines are needed that protect against both infections and do so better than the suboptimal Mycobacterium bovis BCG vaccine. Here, we evaluated rBCG30, a vaccine previously demonstrated to induce protection superior to that of BCG against Mycobacterium tuberculosis and Mycobacterium bovis challenge in animal models, for efficacy against Mycobacterium leprae challenge in a murine model of leprosy. rBCG30 overexpresses the M. tuberculosis 30-kDa major secretory protein antigen 85B, which is 85% homologous with the M. leprae homolog (r30ML). Mice were sham immunized or immunized intradermally with BCG or rBCG30 and challenged 2.5 months later by injection of viable M. leprae into each hind footpad. After 7 months, vaccine efficacy was assessed by enumerating the M. leprae bacteria per footpad. Both BCG and rBCG30 induced significant protection against M. leprae challenge. In the one experiment in which a comparison between BCG and rBCG30 was feasible, rBCG30 induced significantly greater protection than did BCG. Immunization of mice with purified M. tuberculosis or M. leprae antigen 85B also induced protection against M. leprae challenge but less so than BCG or rBCG30. Notably, boosting rBCG30 with M. tuberculosis antigen 85B significantly enhanced r30ML-specific immune responses, substantially more so than boosting BCG, and significantly augmented protection against M. leprae challenge. Thus, rBCG30, a vaccine that induces improved protection against M. tuberculosis, induces cross-protection against M. leprae that is comparable or potentially superior to that induced by BCG, and boosting rBCG30 with antigen 85B further enhances immune responses and protective efficacy. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Insights on the Emergence of Mycobacterium tuberculosis from the Analysis of Mycobacterium kansasii

Science.gov (United States)

Wang, Joyce; McIntosh, Fiona; Radomski, Nicolas; Dewar, Ken; Simeone, Roxane; Enninga, Jost; Brosch, Roland; Rocha, Eduardo P.; Veyrier, Frédéric J.; Behr, Marcel A.

2015-01-01

By phylogenetic analysis, Mycobacterium kansasii is closely related to Mycobacterium tuberculosis. Yet, although both organisms cause pulmonary disease, M. tuberculosis is a global health menace, whereas M. kansasii is an opportunistic pathogen. To illuminate the differences between these organisms, we have sequenced the genome of M. kansasii ATCC 12478 and its plasmid (pMK12478) and conducted side-by-side in vitro and in vivo investigations of these two organisms. The M. kansasii genome is 6,432,277 bp, more than 2 Mb longer than that of M. tuberculosis H37Rv, and the plasmid contains 144,951 bp. Pairwise comparisons reveal conserved and discordant genes and genomic regions. A notable example of genomic conservation is the virulence locus ESX-1, which is intact and functional in the low-virulence M. kansasii, potentially mediating phagosomal disruption. Differences between these organisms include a decreased predicted metabolic capacity, an increased proportion of toxin–antitoxin genes, and the acquisition of M. tuberculosis-specific genes in the pathogen since their common ancestor. Consistent with their distinct epidemiologic profiles, following infection of C57BL/6 mice, M. kansasii counts increased by less than 10-fold over 6 weeks, whereas M. tuberculosis counts increased by over 10,000-fold in just 3 weeks. Together, these data suggest that M. kansasii can serve as an image of the environmental ancestor of M. tuberculosis before its emergence as a professional pathogen, and can be used as a model organism to study the switch from an environmental opportunistic pathogen to a professional host-restricted pathogen. PMID:25716827

Métodos tintoriais utilizados na identificação do Mycobacterium leprae: revisão histológica Staining methods used in the identification of Mycobacterium leprae: historical review

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Luiz Fernando de Góes Siqueira

1984-06-01

Full Text Available Foi feita revisão histórica sobre métodos tintoriais utilizados na identificação baciloscópica do Mycobacterium leprae. Ao lado da descrição de cada método, e suas variantes, é feita extensa revisão bibliográfica.A historical review of the staining methods utilized in the bacilloscopic identification of the Mycobacterium leprae was made. Beside the description of each method and its variants, an extensive bibliographical review is made.

Experimental Inoculation of BFDV-Positive Budgerigars (Melopsittacus undulatus with Two Mycobacterium avium subsp. avium Isolates

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Aleksandra Ledwoń

2014-01-01

Full Text Available Beak and feather disease virus- (BFDV- positive (naturally infected but clinically healthy budgerigars (Melopsittacus undulatus were inoculated with two isolates of Mycobacterium avium subsp. avium isolated from naturally infected golden pheasant (Chrysolophus pictus and peafowl (Pavo cristatus. During a period of more than two months after inoculation, samples of cloacal and crop swabs, faeces, and blood were obtained for BFDV and Mycobacterium avium testing with PCR. Birds were euthanized nine weeks after inoculation. All infected budgerigars developed signs typical of mycobacteriosis, but more advanced clinical and pathological changes were visible in the group infected with the pheasant isolate. Only a few cloacal and crop swab samples were positive for Mycobacterium avium subsp. avium despite advanced pathological changes in the internal organs. In the groups infected with mycobacterium isolates the frequency of BFDV-positive samples was higher than in the control group. In the infected groups the frequency of BFDV was substantially higher in the cloacal swabs of birds inoculated with the pheasant isolate than in the peafowl-isolate-infected group.

Experimental inoculation of BFDV-positive budgerigars (Melopsittacus undulatus) with two Mycobacterium avium subsp. avium isolates.

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Ledwoń, Aleksandra; Sapierzyński, Rafał; Augustynowicz-Kopeć, Ewa; Szeleszczuk, Piotr; Kozak, Marcin

2014-01-01

Beak and feather disease virus- (BFDV-) positive (naturally infected) but clinically healthy budgerigars (Melopsittacus undulatus) were inoculated with two isolates of Mycobacterium avium subsp. avium isolated from naturally infected golden pheasant (Chrysolophus pictus) and peafowl (Pavo cristatus). During a period of more than two months after inoculation, samples of cloacal and crop swabs, faeces, and blood were obtained for BFDV and Mycobacterium avium testing with PCR. Birds were euthanized nine weeks after inoculation. All infected budgerigars developed signs typical of mycobacteriosis, but more advanced clinical and pathological changes were visible in the group infected with the pheasant isolate. Only a few cloacal and crop swab samples were positive for Mycobacterium avium subsp. avium despite advanced pathological changes in the internal organs. In the groups infected with mycobacterium isolates the frequency of BFDV-positive samples was higher than in the control group. In the infected groups the frequency of BFDV was substantially higher in the cloacal swabs of birds inoculated with the pheasant isolate than in the peafowl-isolate-infected group.

Beijing/W genotype Mycobacterium tuberculosis and drug resistance.

NARCIS (Netherlands)

Glynn, Judith R; Kremer, Kristin; Borgdorff, Martien W; Rodriguez, Mar Pujades; Soolingen, Dick van

2006-01-01

Beijing/W genotype Mycobacterium tuberculosis is widespread, may be increasing, and may have a predilection for drug resistance. Individual-level data on >29,000 patients from 49 studies in 35 countries were combined to assess the Beijing genotype's prevalence worldwide, trends over time and with

Modern lineages of Mycobacterium tuberculosis in Addis Ababa ...

African Journals Online (AJOL)

Background: The genotyping of Mycobacterium tuberculosis strains is important to have unique insights into the dissemination dynamics and evolutionary genetics of this pathogen and for TB control as it allows the detection of suspected outbreaks and the tracing of transmission chains. Objective: To characterize M.

Degradation of morpholine by Mycobacterium sp. isolated from ...

African Journals Online (AJOL)

The biodegradation of morpholine has attracted much interest because morpholine causes environmental pollution. Ten species belonging to nine genera were tested for their abilities to degrade morpholine in mineral salts medium containing morpholine (1 g/l). Mycobacterium sp. isolated from polluted water sample ...

Circulatory disease mortality rates in the elderly and exposure to PM2.5 generated by biomass burning in the Brazilian Amazon in 2005 Mortalidad por enfermedades cardiovasculares en los ancianos y la exposición a PM2,5 como resultado de la quema en la Amazonia brasileña en 2005 Mortalidade por doenças circulatórias na população idosa e exposição a PM2,5 em decorrência das queimadas na Amazônia brasileira em 2005

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Eliane Ignotti

2013-03-01

Full Text Available The aim of this study was to analyze the association between the exposure to fine particulate matter and circulatory disease mortality rates in the elderly living in the Brazilian Amazon. An ecological study of circulatory disease, acute myocardial infarction and cerebrovascular disease mortality rates in micro areas of the Brazilian Amazon was carried out. The environmental exposure indicator used was percentage hours of PM2.5 concentrations > 25µg/m³ divided by the total number of estimated hours of PM2.5 in 2005. The association between exposure and circulatory disease mortality rates was strongest in the oldest age group. No significant statistical association was found between cerebrovascular disease mortality rates and exposure. Circulatory disease mortality rates in the elderly living in the Amazon have been influenced by atmospheric pollution from emissions caused by forest fires.El objetivo de este estudio fue analizar la asociación entre la exposición a las partículas finas, con tasas de mortalidad por enfermedades cardiovasculares en los ancianos en la Amazonia brasileña. Se trata de un estudio ecológico de las tasas de mortalidad por enfermedades cardiovasculares, el infarto agudo de miocardio y enfermedades cerebrovasculares en las microrregiones brasileñas de la Amazonia. El indicador de la exposición ambiental fue estimado como un porcentaje de horas de PM2,5 > 25µg/m³, dividido por el número total de horas estimado de PM2,5 en 2005. La asociación del indicador de exposición con las tasas de mortalidad para las enfermedades circulatorias fue mayor en el grupo de mayor edad. La tasa de mortalidad por enfermedad cerebrovascular no se asoció con el indicador de exposición. Las enfermedades cardiovasculares en los ancianos que viven en la Amazonia han sido influenciadas por la contaminación atmosférica, causada por las emisiones de los incendios.O objetivo deste estudo foi analisar a associação da exposição ao

La coloración de Ziehl-Neelsen en histopatología

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Patricia J. de Naranjo

1988-12-01

Full Text Available El diagnóstico definitivo de las enfermedades infecciosas causadas por micobacterias se basa en la demostración de su agente etiológico. Esto se consigue, entre otras formas, por histopatología, mediante la tinción de los bacilos ácido alcohol resistentes (BAAR en los tejidos obtenidos in vivo o en la autopsia.

A Cutaneous Ulcer Resulting from Mycobacterium ulcerans—Leishmania braziliensis Coinfection in South America

Science.gov (United States)

Mougin, Benjamin; Avenel-Audran, Martine; Hasseine, Lilia; Martin, Ludovic; Cottin, Jane; Pomares, Christelle; Delaunay, Pascal; Marty, Pierre; Ravel, Christophe; Chabasse, Dominique; Abgueguen, Pierre

2011-01-01

Buruli ulcer is a tropical skin disease caused by Mycobacterium ulcerans. Its mode of transmission is not yet clearly understood. We report here a cutaneous ulcer in a European traveler in South America resulting from a coinfection detected specifically for Mycobacterium ulcerans and Leishmania braziliensis DNA with real-time polymerase chain reaction. This observation of a unique cutaneous ulcer raises the issue about possible modes of transmission of those two pathogens by the same vector. PMID:22049045

Molecular Epidemiology of Mycobacterium Tuberculosis Strains in ...

African Journals Online (AJOL)

Doroudchi M, Kremer K, Basiri EA, Kadivar MR,. Van Soolingen D, Ghaderi AA. IS6110‑RFLP and spoligotyping of Mycobacterium tuberculosis isolates in Iran. Scand J Infect. Dis 2000;32:663‑8. 13. Farnia P, Masjedi MR, Mirsaeidi M, Mohammadi F,. Jallaledin‑Ghanavi, Vincent V, et al. Prevalence of Haarlem I and Beijing ...

Microevolution of Mycobacterium tuberculosis in a tuberculosis patient.

NARCIS (Netherlands)

Al-Hajoj, S.A.; Akkerman, O.; Parwati, I.; Al-Gamdi, S.; Rahim, Z.; Soolingen, D. van; Ingen, J. van; Supply, P.; Zanden, A.G. van der

2010-01-01

Five Mycobacterium tuberculosis isolates were obtained from three body sites from a Dutch patient. The isolates displayed a single genotype by 24-locus MIRU-VNTR typing (except for a single locus not amplified from one isolate) but were differentiated by small variations in IS6110 fingerprints,

Benzothiazinones kill Mycobacterium tuberculosis by blocking arabinan synthesis

DEFF Research Database (Denmark)

Makarov, Vadim; Manina, Giulia; Mikusova, Katarina

2009-01-01

New drugs are required to counter the tuberculosis (TB) pandemic. Here, we describe the synthesis and characterization of 1,3-benzothiazin-4-ones (BTZs), a new class of antimycobacterial agents that kill Mycobacterium tuberculosis in vitro, ex vivo, and in mouse models of TB. Using genetics...

Efficacy of sulfadoxine-pyrimethamine and mefloquine for the treatment of uncomplicated Plasmodium falciparum malaria in the Amazon basin of Peru Eficácia da sulfadoxina-pirimetamina e mefloquina no tratamento de malária não-complicada por Plasmodium falciparum na bacia amazônica peruana

Directory of Open Access Journals (Sweden)

Alan J. Magill

2004-06-01

Full Text Available In vivo antimalarial drug efficacy studies of uncomplicated Plasmodium falciparum malaria at an isolated site in the Amazon basin of Peru bordering Brazil and Colombia showed >50% RII/RIII resistance to sulfadoxine-pyrimethamine but no evidence of resistance to mefloquine.Testes in vivo foram realizados para avaliar resistência a drogas antimalária, em pessoas com malária não complicada, causada por Plasmodium falciparum, numa região isolada da Bacia Amazônica, na fronteira com o Brasil e a Colômbia. Os testes mostraram resistência >50% RII/RIII a sulfadoxina-pirimetamina, mas não evidenciaram resistência a mefloquina.

Multiplexed Quantitation of Intraphagocyte Mycobacterium tuberculosis Secreted Protein Effectors

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Fadel Sayes

2018-04-01

Full Text Available Summary: The pathogenic potential of Mycobacterium tuberculosis largely depends on ESX secretion systems exporting members of the multigenic Esx, Esp, and PE/PPE protein families. To study the secretion and regulation patterns of these proteins while circumventing immune cross-reactions due to their extensive sequence homologies, we developed an approach that relies on the recognition of their MHC class II epitopes by highly discriminative T cell receptors (TCRs of a panel of T cell hybridomas. The latter were engineered so that each expresses a unique fluorescent reporter linked to specific antigen recognition. The resulting polychromatic and multiplexed imaging assay enabled us to measure the secretion of mycobacterial effectors inside infected host cells. We applied this novel technology to a large panel of mutants, clinical isolates, and host-cell types to explore the host-mycobacteria interplay and its impact on the intracellular bacterial secretome, which also revealed the unexpected capacity of phagocytes from lung granuloma to present mycobacterial antigens via MHC class II. : Sayes et al. develop an approach to express distinct fluorescent reporters that is based on the recognition of specific Mycobacterium tuberculosis MHC class II epitopes by highly discriminative T cell hybridomas. This multiplexed technology allows the study of secretion, subcellular location, and regulation patterns of these instrumental protein members. Keywords: mycobacterium tuberculosis, type VII secretion systems, intracellular bacteria, T-cell hybridomas, mycobacterial virulence factors, bacterial antigen presentation, lentiviral vectors, reporter T cells, in vivo antigen presentation, protein localization

Infection due to Mycobacterium bovis in common variable immunodeficiency

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Diana Andrea Herrera-Sánchez

2015-02-01

Full Text Available Common variable immunodeficiency (CVID is an heterogeneous group of disorders characterized by impaired antibody production. It shows a wide spectrum of manifestations including severe and recurrent respiratory infections (Streptococcus pneumoniae, Haemophilus and gastrointestinal (Campylobacter jejuni, rotavirus and Giardia lamblia. Viral infections caused by herpes zoster, cytomegalovirus (CMV and hepatitis C are rare. The opportunistic agents such as CMV, Pneumocystis jirovecii, cryptococcus and atypical mycobacteria have been reported as isolated cases. This paper reports the case of a 38-year-old female patient, who began six years before with weight loss of 7 kg in six months, fatigue, weakness, sweating, fever and abdominal pain. Furthermore, patient had intestinal obstruction and abdominal CT showed mesenteric lymph growth. The mesenteric lymph node biopsy revealed positives Mycobacterium PCR, Ziehl-Neelsen staining and culture for M. bovis. In the laparotomy postoperative period was complicated with nosocomial pneumonia, requiring mechanical ventilation and tracheostomy. Two years later, she developed right renal abscess that required surgical drainage, once again with a positive culture for Mycobacterium bovis. She was referred to highly specialized hospital and we documented panhypogammaglobulinemia and lymphopenia. Secondary causes of hypogammaglobulinemia were ruled out and common variable immunodeficiency (CVID was confirmed, we started IVIG replacement. Four years later she developed mixed cellularity Hodgkin’s lymphoma. Until today she continues with IVIG and chemotherapy. This report of a patient with CVID and Mycobacterium bovis infection, a unusual association, shows the cellular immunity susceptibility in this immunodeficiency, additional to the humoral defect.

Intraocular manifestations of mycobacterium tuberculosis: A review of the literature

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Lauren A. Dalvin

2017-05-01

Full Text Available Mycobacterium tuberculosis: is most commonly associated with pulmonary infection. However, tuberculosis (TB can also affect the eye. TB can affect nearly any tissue in the eye, and a high index of suspicion is required for accurate diagnosis, as many of the intraocular manifestations of TB can mimic other, more common diseases. Correct diagnosis is critical because systemic anti-tuberculosis treatment may be required, and vision loss or even loss of the affected eye can occur without proper treatment. Thus, it is important for ophthalmologists and infectious disease specialists to work together to accurately diagnose and treat intraocular TB. This article reports the various known presentations of intraocular TB and reviews important elements of diagnosis and treatment. Keywords: Mycobacterium, Tuberculosis, Choroidal granuloma, Retinal vasculitis

Diversity and evolution of drug resistance mechanisms in Mycobacterium tuberculosis

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Al-Saeedi M

2017-10-01

Full Text Available Mashael Al-Saeedi, Sahal Al-Hajoj Department of Infection and Immunity, Mycobacteriology Research Section, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia Abstract: Despite the efficacy of antibiotics to protect humankind against many deadly pathogens, such as Mycobacterium tuberculosis, nothing can prevent the emergence of drug-resistant strains. Several mechanisms facilitate drug resistance in M. tuberculosis including compensatory evolution, epistasis, clonal interference, cell wall integrity, efflux pumps, and target mimicry. In this study, we present recent findings relevant to these mechanisms, which can enable the discovery of new drug targets and subsequent development of novel drugs for treatment of drug-resistant M. tuberculosis. Keywords: Mycobacterium tuberculosis, antibiotic resistance, compensatory evolution, epistasis, efflux pumps, fitness cost

Construction of an internal amplification control for Mycobacterium ...

African Journals Online (AJOL)

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) which mostly affects the lungs. The disease causes deaths of many people every year. There are different methods to detect MTB such as skin test, staining, culture and molecular techniques. Polymerase chain reaction (PCR) is a ...

Advances in the Laboratory Diagnosis of Mycobacterium Tuberculosis

African Journals Online (AJOL)

Mycobacterium tuberculosis (MTB), the agent of human tuberculosis remains a leading cause of mortality globally. Its resurgence during the last two decades is a reflection of its opportunistic relationship with HIV. The challenges associated with the disease are enormous and often debilitating. The role of clinical and ...

Transmissie van Mycobacterium bovis tussen mens en dier

NARCIS (Netherlands)

Vries, de G.; Beer, de J.; Bakker, D.; Soolingen, D.

2015-01-01

Nederland is officieel vrij van rundertuberculose. Toch komt af en toe nog Mycobacterium bovis-tuberculose voor bij relatief jonge autochtone Nederlanders. Ook zijn er recent nog wel boviene-uitbraken geweest. Dat roept de vraag op of er ook nu nog transmissie is van M.bovis tussen mens en dier.

Characterization of a Mycobacterium avium subsp. avium Operon Associated with Virulence and Drug Detoxification

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Mariana Noelia Viale

2014-01-01

Full Text Available The lprG-p55 operon of Mycobacterium tuberculosis and Mycobacterium bovis is involved in the transport of toxic compounds. P55 is an efflux pump that provides resistance to several drugs, while LprG is a lipoprotein that modulates the host's immune response against mycobacteria. The knockout mutation of this operon severely reduces the replication of both mycobacterial species during infection in mice and increases susceptibility to toxic compounds. In order to gain insight into the function of LprG in the Mycobacterium avium complex, in this study, we assayed the effect of the deletion of lprG gene in the D4ER strain of Mycobacterium avium subsp. avium. The replacement of lprG gene with a hygromycin cassette caused a polar effect on the expression of p55. Also, a twofold decrease in ethidium bromide susceptibility was observed and the resistance to the antibiotics rifampicin, amikacin, linezolid, and rifabutin was impaired in the mutant strain. In addition, the mutation decreased the virulence of the bacteria in macrophages in vitro and in a mice model in vivo. These findings clearly indicate that functional LprG and P55 are necessary for the correct transport of toxic compounds and for the survival of MAA in vitro and in vivo.

Buoyant density of Mycobacterium tuberculosis: implications for sputum processing

NARCIS (Netherlands)

den Hertog, A. L.; Klatser, P. R.; Anthony, R. M.

2009-01-01

A tuberculosis (TB) research laboratory in the Netherlands. The concentration of Mycobacterium tuberculosis cells from sputum is almost universally performed by centrifugation after chemical liquefaction. These methods are thus dependent on the effective sedimentation of mycobacterial cells, and the

EVIDENCE FOR THE MACROPHAGE INDUCING GENE IN MYCOBACTERIUM INTRACELLULARE

Science.gov (United States)

Background: The Mycobacterium avium Complex (MAC) includes the species M. avium (MA), M. intracellulare (MI), and possibly others. Organisms belonging to the MAC are phylogenetically closely related, opportunistic pathogens. The macrophage inducing gene (mig) is the only well-des...

Clinical and parasitological profiles of patients with non-complicated Plasmodium falciparum and Plasmodium vivax malaria in northwestern Colombia

OpenAIRE

Knudson-Ospina, Angélica; Sánchez-Pedraza, Ricardo; Pérez-Mazorra, Manuel Alberto; Cortés-Cortés, Liliana Jazmín; Guerra-Vega, Ángela Patricia; Nicholls-Orejuela, Rubén Santiago

2015-01-01

Antecedentes. En Colombia existen pocos estudios que buscan encontrar diferencias clínicas y parasitológicas en la malaria causada por Plasmodium falciparum y Plasmodium vivax. Objetivo. Describir el perfil clínico y parasitológico de las malarias por Plasmodium falciparum y Plasmodium vivax no complicadas en Tierralta, Córdoba, Colombia. Materiales y métodos. Se evaluaron pacientes con paludismo no complicado por Plasmodium falciparum y Plasmodium vivax según los protocolos estandarizados po...

Methylobacterium spp. as an indicator for the presence or absence of Mycobacterium spp.

Science.gov (United States)

Falkinham, Joseph O; Williams, Myra D; Kwait, Rebecca; Lande, Leah

2016-06-01

A published survey of bacteria in showerhead biofilm samples revealed that Methylobacterium spp. and Mycobacterium spp. seldom coexisted in biofilms. To confirm that information, biofilm samples were collected from household plumbing of Mycobacterium avium patients and Methylobacterium spp. and M. avium numbers were measured by direct colony counts. The results demonstrated that if Methylobacterium spp. were present, Mycobacterium spp. were absent, and the opposite. The data demonstrate that microbial populations in biofilms can influence the presence or absence of opportunistic premise plumbing pathogens and, thereby, increase the range of strategies to reduce exposure to waterborne pathogens. Finally, by assessing for the visual presence of methylobacteria as pink pigmentation on showers and shower curtains, homeowners and managers of hospitals and other buildings can quickly determine whether a premise plumbing biofilm sample has mycobacteria with a high degree of assurance. Copyright © 2016 Asian African Society for Mycobacteriology. Published by Elsevier Ltd. All rights reserved.

Nitazoxanide is active against Mycobacterium leprae

Science.gov (United States)

Bailey, Mai Ann; Na, Hana; Duthie, Malcolm S.; Gillis, Thomas P.; Lahiri, Ramanuj

2017-01-01

Nitazoxanide (NTZ) is an anti-parasitic drug that also has activity against bacteria, including Mycobacterium tuberculosis. Our data using both radiorespirometry and live-dead staining in vitro demonstrate that NTZ similarly has bactericidal against M. leprae. Further, gavage of M. leprae-infected mice with NTZ at 25mg/kg provided anti-mycobacterial activity equivalent to rifampicin (RIF) at 10 mg/kg. This suggests that NTZ could be considered for leprosy treatment. PMID:28850614

Evaluation of rapid immuno chromatographic assay kit using monoclonal mpt64 antibodies for identification of mycobacterium tuberculosis complex

International Nuclear Information System (INIS)

Satti, L.; Ikram, A.; Malik, N.

2010-01-01

To evaluate the performance of rapid immuno chromatographic kit MPT64 Ag for the identification of Mycobacterium tuberculosis complex from various Mycobacterium tuberculosis culture positive specimens. Department of Microbiology, Armed Forces Institute of Pathology Rawalpindi, from August 2008 through March 2009. Eighty four Mycobacterium tuberculosis positive cultures on I BACTEC 460 and MGIT 960, one ATCC 25177 MTB strain, three institutional control MTB strains, two institutional control MOTT strains and 20 different bacterial isolates were tested. Tests were performed according to the instructional manual. Out of total 84 tested samples, MPT64 showed positive result in 80 cultures. Only four positive cultures did not display any band on MPT64 kit. These four strains were reconfirmed as Mycobacterium tuberculosis by PCR method. MOTT control strains and all the 20 bacterial isolates were negative for band. The sensitivity and specificity of ICT assay in our study was 95.2% and 100% respectively. Rapid MPT64 Kit is a good diagnostic tool to differentiate between Mycobacterium tuberculosis complex and MOTT with 100% specificity. The technique is simple and can provide prompt information to the clinicians to initiate early and appropriate antituberculosis therapy. (author)

Incidência de infecções por enterobactérias resistentes a carbapenêmicos em pacientes previamente colonizados

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Paula Lüttjohann Rodrigues

2015-09-01

Full Text Available Introdução: Nos últimos anos, a incidência mundial de enterobactérias resistentes a carbapenêmicos (ERC tem aumentado. A disseminação de ERC é preocupante considerando as limitações terapêuticas e a alta mortalidade de infecções causadas por essas bactérias. O objetivo deste estudo foi determinar a incidência de infecções por ERC em pacientes previamente colonizados. Métodos: Estudo de coorte, retrospectivo, realizado em hospital universitário de Porto Alegre, no período de janeiro a dezembro de 2014. Foram incluídos todos os pacientes com coleta de swab retal para pesquisa de ERC. Foram considerados infectados pacientes com isolado de ERC em amostra clínica que apresentaram swab retal prévio positivo para a mesma espécie. A mortalidade bruta foi analisada em 30 dias após o isolamento de ERC. Resultados: Foram coletados 2.733 swabs retais, sendo 78 (2,85% destes positivos para ERC (pacientes colonizados. Um total de 33% (26/78 dos pacientes colonizados também apresentaram ERC em amostra clínica. O tempo médio entre o isolamento de ERC em swab (colonização e em material clínico (infecção foi de 14,9 dias (± 13,6. A maior parte das amostras clínicas positivas para ERC foi em urina (46,1%; 12/26. A mortalidade foi de 61% (16/26 nos pacientes colonizados e com infecção versus 46% (24/52 nos pacientes colonizados que não desenvolveram infecção. Conclusão: Nossos dados demonstram que 1/3 de pacientes colonizados desenvolveram infecção por ERC. Foi possível observar uma mortalidade maior em pacientes infectados em relação a pacientes apenas colonizados por ERC.

Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections.

NARCIS (Netherlands)

Lindeboom, J.A.; Bruijnesteijn van Coppenraet, L.E.; Soolingen, D. van; Prins, J.M.; Kuijper, E.J.

2011-01-01

Mycobacterium haemophilum is a slowly growing acid-fast bacillus (AFB) belonging to the group of nontuberculous mycobacteria (NTM) frequently found in environmental habitats, which can colonize and occasionally infect humans and animals. Several findings suggest that water reservoirs are a likely

Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections

NARCIS (Netherlands)

Lindeboom, J.A.; Bruijnesteijn van Coppenraet, L.E.S.; van Soolingen, D.; Prins, J.M.; Kuijper, E.J.

2011-01-01

Mycobacterium haemophilum is a slowly growing acid-fast bacillus (AFB) belonging to the group of nontuberculous mycobacteria (NTM) frequently found in environmental habitats, which can colonize and occasionally infect humans and animals. Several findings suggest that water reservoirs are a likely

Molecular Characterization of Mycobacterium massiliense and Mycobacterium bolletii in Isolates Collected from Outbreaks of Infections after Laparoscopic Surgeries and Cosmetic Procedures▿

Science.gov (United States)

Viana-Niero, Cristina; Lima, Karla Valéria Batista; Lopes, Maria Luiza; da Silva Rabello, Michelle Christiane; Marsola, Lourival Rodrigues; Brilhante, Vânia Cristina Ribeiro; Durham, Alan Mitchel; Leão, Sylvia Cardoso

2008-01-01

An outbreak of infections affecting 311 patients who had undergone different invasive procedures occurred in 2004 and 2005 in the city of Belém, in the northern region of Brazil. Sixty-seven isolates were studied; 58 were from patients who had undergone laparoscopic surgeries, 1 was from a patient with a postinjection abscess, and 8 were from patients who had undergone mesotherapy. All isolates were rapidly growing nonpigmented mycobacteria and presented a pattern by PCR-restriction enzyme analysis of the hsp65 gene with BstEII of bands of 235 and 210 bp and with HaeIII of bands of 200, 70, 60, and 50 bp, which is common to Mycobacterium abscessus type 2, Mycobacterium bolletii, and Mycobacterium massiliense. hsp65 and rpoB gene sequencing of a subset of 20 isolates was used to discriminate between these three species. hsp65 and rpoB sequences chosen at random from 11 of the 58 isolates from surgical patients and the postinjection abscess isolate presented the highest degrees of similarity with the corresponding sequences of M. massiliense. In the same way, the eight mesotherapy isolates were identified as M. bolletii. Molecular typing by pulsed-field gel electrophoresis (PFGE) grouped all 58 surgical isolates, while the mesotherapy isolates presented three different PFGE patterns and the postinjection abscess isolate showed a unique PFGE pattern. In conclusion, molecular techniques for identification and typing were essential for the discrimination of two concomitant outbreaks and one case, the postinjection abscess, not related to either outbreak, all of which were originally attributed to a single strain of M. abscessus. PMID:18174307

Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil.

Science.gov (United States)

Silva, Marcio Roberto; Rocha, Adalgiza da Silva; da Costa, Ronaldo Rodrigues; de Alencar, Andrea Padilha; de Oliveira, Vania Maria; Fonseca Júnior, Antônio Augusto; Sales, Mariana Lázaro; Issa, Marina de Azevedo; Filho, Paulo Martins Soares; Pereira, Omara Tereza Vianello; dos Santos, Eduardo Calazans; Mendes, Rejane Silva; Ferreira, Angela Maria de Jesus; Mota, Pedro Moacyr Pinto Coelho; Suffys, Philip Noel; Guimarães, Mark Drew Crosland

2013-05-01

In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.

Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil

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Marcio Roberto Silva

2013-05-01

Full Text Available In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis. Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ and Stonebrink (SB-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks. One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6% of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.

Acanthamoeba Sp. S-11 phagocytotic activity on Mycobacterium ...

African Journals Online (AJOL)

Background: Mycobacterium leprae (M. leprae) is a pathogenic bacterium that causes leprosy. The presence of M. leprae in the environment is supported by microorganisms that act as the new host for M. leprae. Acanthamoeba's potential to be a host of M. leprae in the environment. Acanthamoeba sp. is Free Living ...

Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis

DEFF Research Database (Denmark)

Troegeler, Anthony; Lugo-Villarino, Geanncarlo; Hansen, Søren

2015-01-01

Understanding the molecular components of immune recognition of the tuberculosis (TB) bacillus, Mycobacterium tuberculosis, can help designing novel strategies to combat TB. Here, we identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize mycobacte......Understanding the molecular components of immune recognition of the tuberculosis (TB) bacillus, Mycobacterium tuberculosis, can help designing novel strategies to combat TB. Here, we identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize...

Effect of chlorine on Mycobacterium gordonae and Mycobacterium chubuense in planktonic and Biofilm State

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Alejandra Soledad Oriani

2018-01-01

Full Text Available Background: There is evidence that drinking water could be a source of infections with pathogenic nontuberculous mycobacteria (NTM potentially risky to human health. The aim was to investigate the resistance of two NTM isolated from drinking water, Mycobacterium gordonae and Mycobacterium chubuense, at different concentrations of chlorine (as sodium hypochlorite, used in drinking water sanitation. Methods: The NTM were grown in suspension and in biofilms and were challenged with biocide for 10 and 60 min. Results: To obtain 7-log reduction from the initial population of M. chubuense, in the planktonic state, there were necessary 20 ppm of chorine and 60 min of exposure. The same effect was achieved in M. gordonae with 10 ppm for the same period. The maximum reduction of both NTM in biofilm was 3-log reduction and was achieved using 30 ppm for 60 min. The chlorine susceptibility of cells in biofilms was significantly lower than that of planktonic cells. The results highlight the resistance of both NTM to the concentrations used in routine water sanitation (0.2 ppm according to Argentine Food Code. Differences in chlorine resistance found between the two NTM in planktonic growth decrease when they are grown in biofilm. Conclusion: This suggests that current water disinfection procedures do not always achieve effective control of NTM in the public supply system, with the consequent health risk to susceptible population, and the need to take into account biofilms, because of their deep consequences in the way to analyze the survival of prokaryotic cells in different environments.

Expression of Mycobacterium smegmatis pyrazinamidase in Mycobacterium tuberculosis confers hypersensitivity to pyrazinamide and related amides.

Science.gov (United States)

Boshoff, H I; Mizrahi, V

2000-10-01

A pyrazinamidase (PZase)-deficient pncA mutant of Mycobacterium tuberculosis, constructed by allelic exchange, was used to investigate the effects of heterologous amidase gene expression on the susceptibility of this organism to pyrazinamide (PZA) and related amides. The mutant was highly resistant to PZA (MIC, >2,000 microg/ml), in accordance with the well-established role of pncA in the PZA susceptibility of M. tuberculosis (A. Scorpio and Y. Zhang, Nat. Med. 2:662-667, 1996). Integration of the pzaA gene encoding the major PZase/nicotinamidase from Mycobacterium smegmatis (H. I. M. Boshoff and V. Mizrahi, J. Bacteriol. 180:5809-5814, 1998) or the M. tuberculosis pncA gene into the pncA mutant complemented its PZase/nicotinamidase defect. In both pzaA- and pncA-complemented mutant strains, the PZase activity was detected exclusively in the cytoplasm, suggesting an intracellular localization for PzaA and PncA. The pzaA-complemented strain was hypersensitive to PZA (MIC, /=20 microg/ml) and was also sensitive to benzamide (MIC, 20 microg/ml), unlike the wild-type and pncA-complemented mutant strains, which were highly resistant to this amide (MIC, >500 microg/ml). This finding was consistent with the observation that benzamide is hydrolyzed by PzaA but not by PncA. Overexpression of PzaA also conferred sensitivity to PZA, nicotinamide, and benzamide on M. smegmatis (MIC, 150 microg/ml in all cases) and rendered Escherichia coli hypersensitive for growth at low pH.

Embolia pulmonar séptica de origen cutáneo

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Agustina Sosa Beláustegui

2012-08-01

Full Text Available La embolia pulmonar séptica es una enfermedad grave y poco frecuente que se caracteriza por presentar infiltrados pulmonares bilaterales asociados a un foco infeccioso extrapulmonar. Se relaciona principalmente a endocarditis derecha, tromboflebitis pelviana, accesos vasculares y menos frecuentemente a infecciones profundas como osteomielitis, artritis séptica o piomiositis. El Staphylococcus aureus meticilino-resistente adquirido en la comunidad (SAMR-AC es un patógeno emergente, con alta virulencia y de rápida propagación, que afecta a sujetos sin enfermedades previas relacionadas o factores de riesgo conocidos. Causa infecciones de piel y partes blandas y con menor frecuencia infecciones graves como fascitis necrotizante, artritis séptica, osteomielitis, piomiositis y neumonía necrotizante. Su epidemiología, patogenia y manifestaciones clínicas difieren de las causadas por el SAMR adquirido en el hospital. Presentamos el caso de un varón de 67 años con embolias pulmonares sépticas causadas por SAMR-AC con origen en una infección cutánea.

A robust SNP barcode for typing Mycobacterium tuberculosis complex strains

KAUST Repository

Coll, Francesc; McNerney, Ruth; Guerra-Assunç ã o, José Afonso; Glynn, Judith R.; Perdigã o, Joã o; Viveiros, Miguel; Portugal, Isabel; Pain, Arnab; Martin, Nigel; Clark, Taane G.

2014-01-01

Strain-specific genomic diversity in the Mycobacterium tuberculosis complex (MTBC) is an important factor in pathogenesis that may affect virulence, transmissibility, host response and emergence of drug resistance. Several systems have been proposed

Port-site infections by nontuberculous mycobacterium: A retrospective clinico-microbiological study

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Roumi Ghosh

2017-01-01

Full Text Available Background: Port-site infection (PSI is a prevailing, chronic, nagging, treatment refractory complication of laparoscopic surgery (LS. It neutralizes the advantages of minimally invasive surgery and increases morbidity, treatment cost of patient, leading to loss of confidence on operating surgeon. PSIs are preventable with appropriate preoperative, intraoperative, and postoperative measures. Atypical mycobacterium is most commonly associated with nonhealing postlaparoscopic wound infections, causing outbreaks or sporadic cases worldwide. Purpose: We retrospectively studied the occurrence of nontuberculous mycobacterium (NTM from PSIs following LS that did not respond to antibiotics used for pyogenic infections and having sterile routine aerobic cultures and their antimicrobial susceptibility pattern to guide proper management. Methods: The study was done in a tertiary care hospital of Eastern India over a 1-year period which included PSI cases with delayed onset not responding to antibiotics, following different types of LS. Pus/discharge from 32 patients was collected and examined for isolation and identification of the causative agents. Gram stain and Ziehl–Neelsen staining methods were used for direct examination. Culture media included blood agar, Robertson's cooked meat broth, MacConkey agar, and Lowenstein–Jensen medium. Isolates from the cases were identified using biochemical tests or molecular methods and studied the antimicrobial susceptibility pattern by the standard microbiologic procedures. Results: Mycobacterium abscessus (13 and Mycobacterium fortuitum (2 were isolated from 15 serosanguinous drainage obtained from 32 cases by routine microbiological techniques. All isolates analyzed for antimicrobial susceptibility pattern were highly sensitive to clarithromycin (93.3%, amikacin (93.3%, and imipenem (80% but were variable to ciprofloxacin, ofloxacin, and linezolid. Conclusions: Our present study shows frequent association of

Molecular identification of Mycobacterium tuberculosis complex isolates from Kermanshah Province, Iran

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Roghieh Moghaddam

2016-01-01

Full Text Available Tuberculosis is one of the most important zoonotic diseases in the world. Rapid diagnosis of the disease and identification of species is extremely important for proper treatment of the disease as some species of the complex are resistant to the first-line of tuberculosis drugs. The aim of present study was molecular identification of Mycobacterium tuberculosis (MTB complex isolates from Kermanshah Province, Iran, which were submitted to the Tuberculosis Reference Laboratory at Razi Vaccine and Serum Research Institute (Tehran, Iran. To identify the genus Mycobacterium, all isolates were subjected to 16S rRNA polymerase chain reaction (PCR, and PCR-IS6110 was subsequently used to confirm that the isolates belonged to MTB complex. Finally, region of difference (RD typing was used to identify the species in the complex. The results of 16S rRNA and IS6110 PCR analysis showed the presence of 543-bp and 245-bp bands, respectively. Furthermore, 146bp, 172bp, 235bp, and 369bp at RD1, RD4, RD9, and RD12, respectively, were observed during RD typing. Thus, based on the results, all isolates were identified as MTB. It is worth mentioning that most tuberculosis cases are identified on the basis of acid-fast bacilli detection, and antibiotic therapy is immediately initiated subsequently. Moreover, it should be noted that some of these acid-fast positive cases might not be of genus Mycobacterium, and thus, the antibiotics prescribed might threaten the health of the patients. Additionally, if the identified bacilli are not within MTB complex, the drug therapy would differ. However, Mycobacterium bovis, which is a member of MTB complex and is resistant to pyrazinamide, requires exact strain identification. Based on the findings, individual isolates should be identified by RD typing methods, which could clearly discriminate the species from each other.

Multiple brain abscesses due to Penicillium spp infection Abscessos cerebrais múltiplos causados por infecção por Penicillium spp

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Danilo Teixeira Noritomi

2005-06-01

ênero Penicillium. A peniciliose sistêmica é causada pelo P. marneffei e costumava ser uma doença rara, mas atualmente é uma das infecções oportunistas mais comuns em associação com AIDS no Sudeste Asiático. Infecção pelo Penicillium spp de espécie diferente do P. marneffei normalmente causa apenas doenças superficiais ou alérgicas mas doenças invasivas também ocorrem raramente. Nós relatamos o quarto caso de infecção do SNC por Penicillium spp.

FUNGI ASSOCIATED WITH THE DECLINE OF THE MANZANITA (ARCTOSTAPHYLOS PUNGENS HBK. IN THE SIERRA FRIA FROM AGUASCALIENTES STATE, MEXICO

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O. Moreno-Rico

2015-11-01

Full Text Available La manzanita (Arctostaphylos pungens es un arbusto que se encuentra con regularidad en la Sierra Fría del estado de Aguascalientes, México. Este arbusto, al igual que otras plantas silvestres, es afectado por factores bióticos, como las enfermedades, que pueden causar desde daños insignificantes hasta la muerte de las plantas. Los objetivos de este trabajo fueron: 1 identificar los hongos relacionados con el declinamiento de la manzanita en la Sierra Fría de Aguascalientes y 2 conocer la incidencia y severidad de las enfermedades causadas por hongos que afectan tallos y ramas de la manzanita. En cada uno de los sitios visitados se realizó un transecto, de dimensiones variables, en una dirección determinada al azar, que incluyó 100 arbustos, para revisar y estimar: a el porcentaje de plantas sanas, enfermas y muertas, y b la incidencia, severidad y distribución de las enfermedades. Los fitopatógenos se identificaron con base en los síntomas y signos que causan comparándolos con lo reportado en la bibliografía. Las enfermedades más importantes resultaron las causadas por los hongos basidiomicetosInonotus aff. jamaicensisyPhellinus arctostaphyli,que causan pudrición blanca de la madera y cancrosis en la base del tallo y en ramas.Inonotus aff. jamaicensisse encontró en 20 de los 21 sitios muestreados variando su incidencia de 3 a 25%, mientras queP. arctostaphylise encontró en 17 de los 21 sitios muestreados variando su incidencia de 1 a 25%. La severidad de las cancrosis varió de 2 a 44 %. También, se identificaron manchas foliares causadas porHarknessia arctostaphyli,Coccomyces spp., yPassalora spp. Se reporta por primera ocasión la presencia dePhellinus arctostaphylien Aguascalientes. Se reporta por primera vez en México la presencia deInonotus aff. jamaicensis,Harknessia arctostaphyli,Coccomyces spp.yPassalora spp.

MYCOBACTERIUM AVIUM AND DRINKING WATER WHAT ARE THE CONNECTIONS?

Science.gov (United States)

Background: Human Mycobacterium avium infections are only known to be acquired from environmental sources such as water and soil. We compared M. avium isolates from clinical and drinking water sources using molecular tools. Methods: M. avium was isolated from water samples colle...

The transmission of Mycobacterium tuberculosis in high burden settings

NARCIS (Netherlands)

Yates, Tom A.; Khan, Palwasha Y.; Knight, Gwenan M.; Taylor, Jonathon G.; McHugh, Timothy D.; Lipman, Marc; White, Richard G.; Cohen, Ted; Cobelens, Frank G.; Wood, Robin; Moore, David A. J.; Abubakar, Ibrahim

2016-01-01

Unacceptable levels of Mycobacterium tuberculosis transmission are noted in high burden settings and a renewed focus on reducing person-to-person transmission in these communities is needed. We review recent developments in the understanding of airborne transmission. We outline approaches to measure

Host immunity to Mycobacterium tuberculosis and risk of tuberculosis

DEFF Research Database (Denmark)

Michelsen, Sascha Wilk; Soborg, Bolette; Agger, Else-Marie

2016-01-01

BACKGROUND: Human immune responses to latent Mycobacterium tuberculosis (Mtb) infection (LTBI) may enable individuals to control Mtb infection and halt progression to tuberculosis (TB), a hypothesis applied in several novel TB vaccines. We aimed to evaluate whether immune responses to selected LTBI...

Reação hansênica reversa associada à síndrome de reconstituição imune

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Karla Linhares Pinto

2017-08-01

Full Text Available Hanseníase é uma doença causada pelo Mycobacterium leprae que afeta principalmente pele e nervos. Em muitos países subdesenvolvidos, a prevalência de HIV e Hanseníase tem aumentado, consequentemente o número de pessoas coinfectadas também. Relata-se o caso de uma paciente coinfectada por essas duas doenças apresentando quadro de úlceras profundas e muito dolorosas em antebraço direito 3 meses após a introdução de terapia antirretroviral. Após investigação para descartar outras causas de lesões cutâneas ulcerativas, a paciente obteve diagnóstico de reação reversa atípica fazendo parte de um quadro de síndrome de reconstituição imune. A paciente recebeu tratamento com prednisona e obteve resolução do quadro. Apesar da infecção pelo vírus HIV ter baixo impacto no desenvolvimento e evolução da Hanseníase, essa micobacteriose, bem como os quadros reacionais associados a ela, podem se apresentar como manifestação de síndrome de reconstituição imune.

Prototecose: uma doença emergente Protothecosis: an emergent disease

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Expedito K.A. Camboim

2010-01-01

Full Text Available Prototecose, causada por Prototheca zopfii ou P. wickerhamii, é uma doença emergente em animais e humanos. Em bovinos, P. zopfii é uma importante causa de mastite ambiental. Em cães e gatos, a prototecose é causada principalmente por P. zopfii. Em cães, causa infecção cutânea ou uma forma sistêmica envolvendo diversos órgãos. Em gatos, predominam as lesões tegumentares na região da face e plano nasal. No homem, a prototecose, causada principalmente por P. wickerhamii, manifesta-se sob três formas: cutânea, articular com bursite do olécrano e sistêmica. Pode ocorrer em indivíduos imunocompetentes, os quais podem apresentar bursite e/ou infecções cutâneas localizadas, ou em indivíduos imunossuprimidos, nos quais a enfermidade pode ser disseminada e/ou com envolvimento visceral. A prototecose causada por P. wickerhamii foi descrita recentemente em caprinos como causa de rinite afetando o vestíbulo nasal, união mucocutânea, pele da face e orelha. Nesta revisão são abordadas as características microbiologias e susceptibilidade a antimicrobianos do agente, e a epidemiologia, sinais clínicos, patologia, diagnóstico, tratamento e controle da prototecose em animais domésticos e no homem.Protothecosis, caused by Prototheca zopfii or P. wickerhamii, is an emergent disease of human and animals. In cattle, P. zopfii is an important cause of environmental mastitis. In dogs and cats protothecosis is caused mainly by P. zopfii, causing cutaneous infections or a systemic form affecting many organs in dogs, and cutaneous infection affecting mainly the skin of the face and nose in cats. In humans, protothecosis, caused mainly by P. wickerhamii, occurs in three forms: cutaneous; olecran bursitis; and disseminated. The lesion is usually localized in the site of inoculation in immunocompetent individuals; however, in immunocompromised patients, it can become widespread. Protothecosis caused by P. wickerhamii was recently reported in

Characterization of drug susceptibility of Mycobacterium tuberculosis isolated from new cases of tuberculosis concurrent with HIV infection

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G. V. Panov

2015-01-01

Full Text Available The paper characterizes drug susceptibility in Mycobacterium tuberculosis isolated from new cases of tuberculosis concurrent with HIV infection. The investigators have studied the spectrum of drug resistance in Mycobacterium tuberculosis isolated from new cases of tuberculosis concurrent with and without HIV infection (172 and 309 clinical isolates, respectively. There are differences in the rate of primary drug resistance to antituberculosis drugs in patients with and without HIV infection (59 and 43.5% of the cases, respectively. The HIV-infected have also shown high rifampicin resistance rates in Mycobacterium tuberculosis (41.7%. The reasons for these differences are as yet unknown and call for further investigation.

Mycobacterium smegmatis infection of a prosthetic total knee arthroplasty.

Science.gov (United States)

Saffo, Zaid; Ognjan, Anthony

2016-01-01

The most common organisms causing prosthetic knee joint infections are staphylococci. However, arthroplasty infections with atypical microbial pathogens, such as Mycobacteria can occur. Due to the rarity of mycobacterial prosthetic joint infections, diagnosis, treatment, and management of these atypical infections represent a clinical challenge. A 71-year old female post-operative day 40 after a left total knee arthroplasty was hospitalized secondary to left knee pain and suspected arthroplasty infection. She had failed outpatient oral antimicrobial treatment for superficial stitch abscess; and outpatient IV/Oral antimicrobials for a clinical postoperative septic bursitis. Ultimately, resection arthroplasty with operative tissue acid fast bacterial cultures demonstrated growth of the Mycobacterium smegmatis group. Post-operatively, she completed a combination course of oral doxycycline and levofloxacin and successfully completed a replacement arthroplasty with clinical and microbial resolution of the infection. To our knowledge, literature review demonstrates three case of knee arthroplasty infection caused by the Mycobacterium smegmatis group. Correspondingly, optimal surgical procedures and antimicrobial management including antimicrobial selection, treatment duration are not well defined. Presently, the best treatment options consists of two step surgical management including prosthesis hardware removal followed by extended antimicrobial therapy, followed by consideration for re-implantation arthroplasty. Our case illustrates importance of considering atypical mycobacterial infections in post-operative arthroplasty infections not responding to traditional surgical manipulations and antimicrobials. For an arthroplasty infection involving the atypical Mycobacterium smegmatis group, two step arthroplasty revision, including arthroplasty resection, with a combination of oral doxycycline and levofloxacin can lead to successful infection resolution, allowing for a

El Antracnosis o Aceituna jabonosa en el cultivo del olivo

OpenAIRE

Trapero Casas, Antonio

2003-01-01

La Antracnosis del olivo, conocida comúnmente como Aceituna jabonosa, es causada por el hongo "Colletotrichum gloeosporioides" y se desarrolla principalmente en las zonas húmedas del sur y noreste peninsular. Esta enfermedad fúngica afecta tanto a frutos como hojas y ramas.

Características clínicas de la pancreatitis aguda en México

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J.A. González-González

2012-10-01

Conclusiones: La etiología de la PA más frecuente es la biliar, pero la causada por alcohol tiene más complicaciones. La mortalidad global fue del 5,00% y ésta se incremento en los pacientes de mayor edad.

Disease caused by non-tuberculous mycobacteria: diagnostic procedures and treatment evaluation in the North of Buenos Aires Province

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Belén Imperiale

2012-03-01

Full Text Available Non-tuberculous mycobacteria (NTM have emerged as pathogens frequently associated to HIV co-infection. The aims of this study were to describe the clinical importance of NTM in patients from the North of Buenos Aires Province and the drug-susceptibility patterns in relation with the therapy used. A total of 23,624 clinical specimens were investigated during the period 2004-2010. Ziehl-Neelsen stain and cultures were used for diagnosis. Molecular and biochemical tests were performed to identify the mycobacteria. TB and mycobacterioses cases were 2 118 and 108 respectively. Sixteen NTM species were found: Mycobacterium avium and Mycobacterium intracellulare as the main causative agents. Infections produced by more than one species at the same time were confirmed (4 cases. Macrolides and fluoroquinolones were the most active in vitro drugs. Treatment evaluation showed that 68.0 % of the cases completed the therapy, 20 % died; and 12 % were relapses. The cases in which the treatment outcome was evaluated received an individual tailor-made therapeutic scheme including those drugs showing in vitro activity and presumed in vivo usefulness. More than a quarter of the patients had HIV co-infection and the majority of the deaths were associated with this co-infection.Enfermedad causada por micobacterias no tuberculosas: diagnóstico y evaluación del tratamiento en el norte del Gran Buenos Aires. Las micobacterias no tuberculosas (MNT emergieron como patógenos frecuentemente asociados a la co-infección con el HIV. EL objetivo del estudio fue describir la importancia clínica de las MNT en pacientes de la región norte de la provincia de Buenos Aires y los patrones de drogo-sensibilidad en relación con la terapia empleada. Se investigó un total de 23.624 especímenes clínicos durante, el período 2004-2010. La tinción de Ziehl-Neelsen y los cultivos se utilizaron para diagnóstico. Las micobacterias fueron identificadas mediante pruebas bioqu

Control de la antracnosis en el cultivo de faba granja asturiana

OpenAIRE

Pérez, Elena; Campa, Ana; Ferreira, J.J. (Juan)

2010-01-01

La antracnosis, causada por el hongo "Colletotrichum lindemuthianum" (Sacc. et Magn.) Scrib., se distribuye por todo el mundo pero está especialmente presente en zonas de temperatura moderada y elevada humedad como el norte de España. Este artículo resume las estrategias para el control de la antracnosis en el cultivo de faba e incluye una descripción de las variedades resistentes a esta enfermedad desarrolladas en el SERIDA por mejora genética clásica.

Evidence of presence of Mycobacterium tuberculosis in bovine tissue samples by multiplex PCR: possible relevance to reverse zoonosis.

Science.gov (United States)

Mittal, M; Chakravarti, S; Sharma, V; Sanjeeth, B S; Churamani, C P; Kanwar, N S

2014-04-01

Bovine tuberculosis, caused by Mycobacterium bovis, remains one of the most important zoonotic health concerns worldwide. The transmission of Mycobacterium tuberculosis from humans to animals also occurs especially in countries where there is close interaction of humans with the animals. In the present study, thirty bovine lung tissue autopsy samples from an organized dairy farm located in North India were screened for the presence of Mycobacterium tuberculosis complex by smear microscopy, histopathological findings and PCR. Differential diagnosis of M. tuberculosis and M. bovis was made based on the deletion of mce-3 operon in M. bovis. The present study found eight of these samples positive for M. tuberculosis by multiplex PCR. Sequencing was performed on two PCR-positive representative samples and on annotation, and BLAST analysis confirmed the presence of gene fragment specific to Mycobacterium tuberculosis. The presence of M. tuberculosis in all the positive samples raises the possibility of human-to-cattle transmission and possible adaptation of this organism in bovine tissues. This study accentuates the importance of screening and differential diagnosis of Mycobacterium tuberculosis complex in humans and livestock for adopting effective TB control and eradication programmes. © 2014 Blackwell Verlag GmbH.

Circumvention of the Mycobactin Requirement of Mycobacterium paratuberculosis

Science.gov (United States)

Morrison, Norman E.

1965-01-01

Morrison, Norman E. (Johns Hopkins University-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Md.). Circumvention of the mycobactin requirement of Mycobacterium paratuberculosis. J. Bacteriol. 89:762–767. 1965.—The mycobactin growth requirement of Mycobacterium paratuberculosis was circumvented on glucose-containing synthetic medium with an initial pH of 5.5. Mycobactin was required during the first transfer on the synthetic medium. Subsequent transfers have grown in the absence of mycobactin. The growth of mycobactin-“independent” strains of M. paratuberculosis on the synthetic medium was found to be stimulated by low concentrations of mycobactin. The circumvention of the mycobactin requirement appears to depend upon the properties of the medium and not upon having created conditions which promote endogenous mycobactin synthesis. Investigation of the glucose-containing synthetic medium showed that: (i) growth stimulatory compounds were formed during autoclaving, and (ii) compared with neutrality a pH of 5.5 gave markedly increased pellicle yields. It was suggested that the growth-stimulatory compounds formed during autoclaving may in part be responsible for the circumvention of the mycobactin requirement. PMID:14273658

Determinación de la susceptibilidad a drogas de primera línea en aislamientos de mycobacterium tuberculosis por la técnica del tubo indicador de crecimiento micobacteriano

OpenAIRE

Sierra Parada, Claudia Rocío; Sánchez Morales, Édgar Alberto; Henao Riveros, Sandra Consuelo; Saavedra Rodríguez, Alfredo

2010-01-01

Antecedentes. La aparición de aislamientos de Mycobacterium tuberculosis resistentes a los medicamentos ha hecho que se busquen métodos más rápidos y confiables para la determinación de la susceptibilidad a las drogas antituberculosas. Objetivo. Determinación de la susceptibilidad a drogas antituberculosas de primera línea en aislamientos de M. tuberculosis utilizando el método del tubo indicador de crecimiento micobacteriano (MGIT). Material y métodos. Se estudiaron 49 aislamientos...

Avaliação de danos por insetos em toras estocadas em indústrias madeireiras de Manaus, Amazonas, Brasil

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Abreu Raimunda Liége Souza de

2002-01-01

Full Text Available Em seis indústrias madeireiras de Manaus, Amazonas, foi realizado um trabalho de pesquisa, com a utilização de um questionário,para averiguar as condições de uso e processamento da madeira e as medidas preventivas contra o ataque de insetos. Foram realizados, também,um levantamento da ocorrência de insetos em 19 espécies de madeiras utilizadas por essas indústrias e a avaliação do dano provocado pelas principais espécies de Coleoptera (besouros e Isoptera (cupins. Das respostas apuradas, constatou-se que nenhuma das empresas visitadas emprega qualquer produto para prevenir o ataque de insetos às toras, assim como a secagem e a estocagem das toras são feitas de forma incorreta, contribuindo para aumentar a intensidade de ataque de insetos. Foram encontradas uma família de cupins e 16 de besouros, ressaltando que destas apenas cinco causam danos à madeira. Do total de 13 espécies de insetos coletados, destacam-se Xyleborus affinis Eichhoff e Platypus parallelus (Fabricius, encontradas em 18 espécies madeireiras, sendo conseqüentemente responsáveis pela maioria dos danos nas toras X. volvulus (Fabricius e Platypus sp. foram encontradas em cinco espécies; X. ferrugineus (Fabricius em três espécies; Minthea rugicolis Walk, Minthea sp. e Nasutitermes corniger (Motschulsky em duas, e Dinoderus bifoveolatus Wollaston, Anoplotermes sp.; e Cnesinus sp. em uma. As espécies de madeiras que sofreram maior grau de deterioração, causada principalmente por coleópteros, foram Ceiba pentandra (L. Gaertn. e Copaifera multijuga Hayne, seguidas por Couroupitaguianensis Aubl., Calophyllum brasiliense Cambess., Cedrela odorata L., Hevea brasiliensis Müll. Arg., Hura crepitans L., Hymenolobium sp., Maquira coriacea (Karsten C.C. Berg, Nectandra sp., Virolasurinamensis Warb. e Vochysia sp.

Correlation of bacterial viability with uptake of (14C) acetate into phenolic glycolipid-1 of Mycobacterium leprae within Schwannoma cells

International Nuclear Information System (INIS)

Mistry, Y.; Antia, N.H.; Mukherjee, R.

1989-01-01

The viability of Mycobacterium leprae, maintained within 33B Schwannoma cells, was estimated in terms of incorporation of ( 14 C) acetate into its specific phenolic glycolipid-1. This measure of viability was correlated with two other assays, viz., fluorescein diacetate/ethidium bromide staining and mouse footpad growth. Observation of a 2-fold increase in the number of intracellular Mycobacterium leprae over an experimental period of 12 days also corroborated this contention. Furthermore, on addition of anti-leprosy drugs to these intracellular Mycobacterium leprae there was significant decrease in phenolic glycolipid-1 synthesis indicative of loss of viability of the organisms. This study also established the importance of the host cell for active bacillary metabolism, as Mycobacterium leprae maintained in cell-free conditions showed no incorporation into phenolic glycolipid-1. Moreover, compromising the host's protein synthesis capacity with cycloheximide, also led to reduction in bacillary metabolism. As this system measures the metabolic synthesis of a unique Mycobacterium leprae component, it would be useful for development and screening of compounds acting against specific bacillary targets. (author). 19 refs., 5 tabs

Avaliação das perdas causadas por vírus na produção da batata I: Vírus do enrolamento da fôlha Evaluation of yield losses induced by potato leaf roll

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F. P. Cupertino

1970-01-01

Full Text Available A comparação entre as produções de 50 pares de plantas sadias e afetadas, de nove variedades de batata, mostra que a redução na produção causada pela infecção secundária do vírus do enrolamento da fôlha foi, em média, de 60,8% no pêso total dos tubérculos produzidos e de 75,5% no pêso dos tubérculos do tipo graúdo ("especial" mais "primeira". As perdas na produção total variaram de 44,6% (variedade Patrones a 73,4% (variedade Aquila, e na de tubérculos graúdos, de 49,8% (Delta A a 86,0% (Gunda.A comparison of 50 pairs of field healthy and leaf roll infected potato plants of nine varieties indicated that the total yield reduction was on the average 60.7 per cent. Yield reduction of the two largest and most marketable potato sizes ("especial" and "primeira" reached 75.6 per cent. Aquila and Gunda had the highest yield losses (73.4 and 72.0 per cent; Delta A and Patrones had the lowest (47.6 and 44.6 per cent. Reduction in the yield of the larger tuber sizes was generally greater than total losses.

Sensitivity of Mycobacterium bovis to common beef processing interventions

Science.gov (United States)

Objective. Mycobacterium bovis is the causative agent of bovine tuberculosis, a relevant zoonosis that can spread to humans through inhalation or by ingestion. M. bovis multiplies slowly, so infected animals may be sent to slaughter during the early stages of the disease before diagnosis and when ...

Prevalence of Mycobacterium bovis in Cattle Slaughtered at Sokoto ...

African Journals Online (AJOL)

This study was undertaken to screen cattle slaughtered at the Sokoto Central Abattoir for antibodies against Mycobacterium bovis. By the lateral flow technique (immunochromatography), using monoclonal antibodies for M. bovis (BioNote, Inc. Gyeonggi-do, Korea) and by post mortem examination. A total of 194 slaughtered ...

Por trás de adenopatias generalizadas

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Liliana Branco

2016-06-01

Full Text Available Introdução: As adenopatias são frequentes na população pediátrica. Geralmente são secundárias a processos benignos e auto-limitados, mas podem associar-se a situações de maior gravidade. Quando ocorre envolvimento de duas ou mais regiões ganglionares não contíguas é classificada como generalizada, sendo a doença sistémica a causa mais comum. Caso clínico: Adolescente do sexo masculino, 16 anos de idade, admitido no serviço de urgência (SU por tumefação axilar à esquerda com 4 dias de evolução. Sem sintomas sistémicos associados. Negava conviventes doentes ou viagens recentes. De salientar apenas o contato com animais (cão e gato. Ao exame objetivo apresentava adenomegalias na região cervical (bilaterais com cerca de 2 cm de maior diâmetro à esquerda, supraclavicular (à esquerda com cerca de 1 cm de maior diâmetro e axilar (bilaterais com cerca de 1,5 cm de maior diâmetro à esquerda, de consistência elástica, bordos regulares, indolores à palpação e não aderentes aos planos superficiais e profundos. O estudo analítico e a radiografia torácica não revelaram alterações de relevo. Efetuou tomografia computorizada (TC tóraco-abdominopélvica que confirmou a presença de adenopatias generalizadas nomeadamente axilares, mediastínicas, retroperitoneais e mesentéricas. De salientar marcadores víricos negativos. O estudo serológico revelou tratar-se de uma infeção aguda portoxoplasmose, pela presença de IgG e de IgM positivas (>250 UI/L e >10.000 respetivamente. Em consulta de seguimento, foi documentado o decréscimo da IgM (7,66 Index tendo-se verificado concomitantemente uma resolução progressiva e espontânea da sintomatologia. Conclusão: As adenopatias generalizadas estão associadas a inúmeras etiologias constituindo um desafio diagnóstico. A toxoplasmose é uma infeção causada por um parasita intracelular, o Toxoplasma gondii (T.gondii, e a serologia é o teste mais utilizado para

In vitro activity of cefoxitin and imipenem against Mycobacterium abscessus complex.

Science.gov (United States)

Lavollay, M; Dubée, V; Heym, B; Herrmann, J-L; Gaillard, J-L; Gutmann, L; Arthur, M; Mainardi, J-L

2014-05-01

The in vitro activity of cefoxitin and imipenem was compared for 43 strains of the Mycobacterium abscessus complex, mostly isolated from cystic fibrosis patients. The MICs of imipenem were lower than those of cefoxitin, although the number of imipenem-resistant strains was higher according to the CLSI breakpoints. Strain comparisons indicated that the MICs of cefoxitin were significantly higher for Mycobacterium bolletii than for M. abscessus. The MICs of both β-lactams were higher for the rough morphotype than for the smooth morphotype. The clinical impact of the in vitro difference between the activity of imipenem and that of cefoxitin remains to be determined. © 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases.

Enfisema subcutáneo en mano y antebrazo por aire comprimido

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M. Klein

2003-12-01

Full Text Available Las lesiones cutáneas que se acompañan de crepitación sugieren la sospecha de infecciones causadas por gérmenes productores de gas. Las causas no infecciosas no son tan reconocidas y suelen tener buen pronóstico. Presentamos un paciente de 27 años que consultó por hinchazón de la mano y antebrazo izquierdo luego de sufrir una lesión cutánea punzante en un dedo, y de secar el área con aire comprimido. En el examen se comprobó un extenso enfisema subcutáneo desde la mano hasta el codo. Una radiografía reveló la presencia de gas subcutáneo en la mano y antebrazo. El enfisema subcutáneo desapareció en 48 horas. El enfisema subcutáneo es siempre un signo preocupante y casos benignos como este se deben considerar en el diagnóstico diferencial. La exposición de lesiones preexistentes a gases comprimidos (compresores y herramientas neumáticas, tubos de gases para distintos usos, armas de aire comprimido identifica la causa y evita eventuales procedimientos quirúrgicos innecesarios.Cutaneous injuries with crepitus suggest infections caused by gas forming organisms. The non infectious causes of subcutaneous emphysema are underrecognized, and are usually of good prognosis. A 27 year old man presented a swollen hand and forearm. He had suffered a small puncture wound in a finger. He had cleaned and dried the area briefly with compressed air, developing a fast swelling of this hand and forearm. At examination, we found extended subcutaneous emphysema in a radiography and observed subcutaneous air in the hand and forearm; 48 hs later there was no evidence of subcutaneous air. The presence of subcutaneous emphysema is always worrisome and such cases must be considered in the differential diagnosis. The exposition of preexistent wounds to compressed air sources (high pressure pneumatic tools, scuba compressed air tubes, compressed air guns identifies the cause and prevents unnecessary surgical interventions.

Cerebelite aguda causada por vírus Epstein-Barr: relato de caso

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Teive Hélio A.G.

2001-01-01

Full Text Available A cerebelite aguda pode ocorrer em associação a infecção pelo vírus da varicela-zoster, enterovirus, caxumba, micoplasma e outros agentes infecciosos. A cerebelite aguda é uma complicação rara da infecção pelo vírus Epstein-Barr (EBV. Relatamos o caso de uma mulher de 21 anos com história de 12 dias de evolução com náuseas, vômitos, ataxia de marcha e membros, tremor cefálico e de membros, opsoclono, mioclonias e rash cutâneo. Sorologia para EBV foi positiva. A infecção pelo EBV, com complicações neurológicas, pode não se apresentar com os sinais e sintomas clássicos da mononucleose infeciosa.