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Sample records for candidiasis

  1. Candidiasis

    ... Foodborne, Waterborne, and Environmental Diseases Mycotic Diseases Branch Candidiasis Recommend on Facebook Tweet Share Compartir Photomicrograph of ... topics, visit the fungal diseases homepage. Types of Candidiasis Thrush (“Oropharyngeal / Esophageal Candidiasis”) Vaginal Yeast Infections ( ...

  2. Invasive Candidiasis

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  3. Candidiasis (vulvovaginal)

    2010-01-01

    Vulvovaginal candidiasis is characterised by vulval itching and abnormal "cheese-like" or watery vaginal discharge. Vulvovaginal candidiasis is estimated to be the second most common cause of vaginitis after bacterial vaginosis. Candida albicans accounts for 85% to 90% of cases.Risk factors include pregnancy, diabetes mellitus, and systemic antibiotics. Incidence increases with the onset of sexual activity, but associations with different types of contraceptives are unclear.Recurrent sympt...

  4. Oral candidiasis.

    Millsop, Jillian W; Fazel, Nasim

    2016-01-01

    Oral candidiasis (OC) is a common fungal disease encountered in dermatology, most commonly caused by an overgrowth of Candida albicans in the mouth. Although thrush is a well-recognized presentation of OC, it behooves clinicians to be aware of the many other presentations of this disease and how to accurately diagnose and manage these cases. The clinical presentations of OC can be broadly classified as white or erythematous candidiasis, with various subtypes in each category. The treatments include appropriate oral hygiene, topical agents, and systemic medications. This review focuses on the various clinical presentations of OC and treatment options. PMID:27343964

  5. Gastro-oesophageal candidiasis.

    Scott, B B; D. Jenkins(University of York, UK)

    1982-01-01

    A prospective search for gastro-oesophageal candidiasis was made by histological examination of all the biopsies taken from 465 patients endoscoped consecutively during a 12 month period. The criterion for diagnosis was the demonstration of infiltration of tissue or ulcer slough by yeasts and hyphae. Nineteen cases of candidiasis were found giving an overall incidence of 4%. There were 12 cases with oesophageal candidiasis, two with both oesophageal and gastric candidiasis, and five with gast...

  6. Oropharyngeal candidiasis and radiotherapy

    The oropharyngeal candidiasis is a common condition in cancer patients treated by irradiation, during and after their treatment. For example, almost 70% of patients treated with chemo-radiation for head and neck cancer are colonized, and 40% of symptomatic patients have an oropharyngeal candidiasis. Furthermore, we noticed an increase in non-albicans Candida strains, which are present in almost 50% of samples. Cancer treatments, especially radiation therapy, and co-morbidities are risk factors of oropharyngeal candidiasis. Oropharyngeal candidiasis has substantial effects on quality of life, and may limit treatment. Epidemiologic data, physiopathology, clinical diagnosis criteria, consequences and treatment of oropharyngeal candidiasis will be discussed in this article. (authors)

  7. Thrush (Oral Candidiasis) in Adults

    ... rashes clinical tools newsletter | contact Share | Thrush (Oral Candidiasis) Information for adults A A A White, slightly ... the tongue and lips are typical of oral candidiasis. Overview Thrush (oral candidiasis), also known as oral ...

  8. Animal Models for Candidiasis

    Conti, Heather R.; Huppler, Anna R.; Whibley, Natasha; Gaffen, Sarah L

    2014-01-01

    Multiple forms of candidiasis are clinically important in humans. Established murine models of disseminated, oropharyngeal, vaginal, and cutaneous candidiasis caused by Candida albicans are described in this unit. Detailed materials and methods for C. albicans growth and detection are also described.

  9. Diaper Dermatitis (Candidiasis)

    ... and rashes clinical tools newsletter | contact Share | Diaper Dermatitis (Candidiasis) A parent's guide for infants and babies ... is dirty (has fecal contamination), an inflammatory skin rash (dermatitis) may develop on the baby's skin. Diarrhea ...

  10. Yeast Infection (Candidiasis)

    ... rash and rashes clinical tools newsletter | contact Share | Yeast Infection (Candidiasis) Information for adults A A A This is a candida (yeast) infection of the skin folds of the abdomen. Overview ...

  11. Oropharyngeal/Esophageal Candidiasis ("Thrush")

    ... is called Candida esophagitis, or esophageal candidiasis. Symptoms Candida infections of the mouth and throat can manifest in ... these symptoms. Risk & Prevention Who Gets Oral Candidiasis? Candida infections of the mouth and throat are uncommon among ...

  12. Oesophageal candidiasis after omeprazole therapy.

    Larner, A J; Lendrum, R

    1992-01-01

    Oesophageal candidiasis was diagnosed incidentally at endoscopy in two patients receiving omeprazole therapy. There were no other predisposing factors for the development of candidiasis. The infection was resolved rapidly by anti-candidal therapy and by stopping omeprazole. These findings suggest that gastric acid secretion and physiological reflux of acid into the oesophagus may play a protective role in preventing candida infection.

  13. Neonatal systemic candidiasis treated miconazole.

    Tuck, S

    1980-01-01

    Two premature newborn infants with systemic candidiasis are reported; both were treated with miconazole. One died and the other made a complete recovery. Miconazole may be a useful addition to the drugs available for the treatment of systemic candidiasis in the neonate, but all of them have serious limitations.

  14. Recurrent vulvovaginal candidiasis.

    Sobel, Jack D

    2016-01-01

    Recurrent vulvovaginal candidiasis (RVVC) is a common cause of significant morbidity in women in all strata of society affecting millions of women worldwide. Previously, RVVC occurrence was limited by onset of menopause but the widespread use of hormone replacement therapy has extended the at-risk period. Candida albicans remains the dominant species responsible for RVVC, however optimal management of RVVC requires species determination and effective treatment measures are best if species-specific. Considerable progress has been made in understanding risk factors that determine susceptibility to RVVC, particularly genetic factors, as well as new insights into normal vaginal defense immune mechanisms and their aberrations in RVVC. While effective control of RVVC is achievable with the use of fluconazole maintenance suppressive therapy, cure of RVVC remains elusive especially in this era of fluconazole drug resistance. Vaccine development remains a critical challenge and need. PMID:26164695

  15. Cerebral candidiasis. Computed tomography appearance

    A three year old child who had been suffering from oral candidiasis since the age of 1 year presented with osteitis of the clavicle, 2 cerebral frontal abscesses and an occipital abscess which extended across the calvaria and was associated with osteolysis. Histological and microbiological studies following surgery confirmed the diagnosis of candidiasis in this girl who was found to have IgA immunodefinciency. The authors report the computed tomographic appearance of the cerebral lesions and review the literature. (orig.)

  16. Cerebral candidiasis. Computed tomography appearance

    Chaabane, M.; Ladeb, M.F.; Bouhaouala, M.H.; Ben Hammouda, M.; Ataalah, R.; Gannouni, A.; Krifa, H.

    1989-07-01

    A three year old child who had been suffering from oral candidiasis since the age of 1 year presented with osteitis of the clavicle, 2 cerebral frontal abscesses and an occipital abscess which extended across the calvaria and was associated with osteolysis. Histological and microbiological studies following surgery confirmed the diagnosis of candidiasis in this girl who was found to have IgA immunodefinciency. The authors report the computed tomographic appearance of the cerebral lesions and review the literature. (orig.).

  17. Disseminated candidiasis 18 years after renal transplantation

    Bismay, K.; Mathew, A.; R. Rajesh; Kurian, G.; Unni, V. N.; Kavita, R. D.; Sreehari, S.

    2012-01-01

    Although mucocutaneous candidiasis is a common infection in renal transplant recipients, disseminated candidiasis is rare. Candida pnemonia causing miliary mottling on X-ray chest with the central nervous system involvement is still rarer. We report an unusual case with disseminated candidiasis that presented 18 years after renal transplantation and improved on conventional antifungal therapy; the relevant literature is reviewed.

  18. ORAL CANDIDIASIS AND HIV INFECTION

    Prabhu, Rachana Vishnudas

    2013-01-01

    The acquired immunodeficiency syndrome (AIDS) pandemic, caused by infection with human immunodeficiency virus (HIV) dramatically illustrates the awesome transmission capabilities of disease. Oral manifestations of HIV have been important in identification of patients harboring the HIV virus and in predicting the decline in their immune system Oral candidiasis is one of the earliest premonitory signs of HIV infection and its diagnosis may have grave prognostic implications for the eventual dev...

  19. Immunopathogenesis of recurrent vulvovaginal candidiasis.

    Fidel, P. L.; Sobel, J D

    1996-01-01

    Recurrent vulvovaginal candidiasis (RVVC) is a prevalent opportunistic mucosal infection, caused predominantly by Candida albicans, which affects a significant number of otherwise healthy women of childbearing age. Since there are no known exogenous predisposing factors to explain the incidence of symptomatic vaginitis in most women with idiopathic RVVC, it has been postulated that these particular women suffer from an immunological abnormality that prediposes them to RVVC. Because of the inc...

  20. Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy

    Sonal, Choudhary; Michael, McLeod; Daniele, Torchia; Paolo, Romanelli

    2012-01-01

    Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy is a rare autoimmune disorder. The clinical spectrum of symptoms is diverse; the diagnosis relying on the presence of at least two out of the three main conditions defining the syndrome: chronic mucocutaneous candidiasis, hypoparathyroidism, and Addison's disease.

  1. Risk prediction for invasive candidiasis

    Armin Ahmed

    2014-01-01

    Full Text Available Over past few years, treatment of invasive candidiasis (IC has evolved from targeted therapy to prophylaxis, pre-emptive and empirical therapy. Numerous predisposing factors for IC have been grouped together in various combinations to design risk prediction models. These models in general have shown good negative predictive value, but poor positive predictive value. They are useful in selecting the population which is less likely to benefit from empirical antifungal therapy and thus prevent overuse of antifungal agents. Current article deals with various risk prediction models for IC and their external validation studies.

  2. Immunopathogenesis of recurrent vulvovaginal candidiasis.

    Fidel, P L; Sobel, J D

    1996-07-01

    Recurrent vulvovaginal candidiasis (RVVC) is a prevalent opportunistic mucosal infection, caused predominantly by Candida albicans, which affects a significant number of otherwise healthy women of childbearing age. Since there are no known exogenous predisposing factors to explain the incidence of symptomatic vaginitis in most women with idiopathic RVVC, it has been postulated that these particular women suffer from an immunological abnormality that prediposes them to RVVC. Because of the increased incidence of mucosal candidiasis in individuals with depressed cell-mediated immunity (CMI), defects in CMI are viewed as a possible explanation for RVVC. In this review, we attempt to place into perspective the accumulated information regarding the immunopathogenesis of RVVC, as well as to provide new immunological perspectives and hypotheses regarding potential immunological deficiencies that may predispose to RVVC and potentially other mucosal infections by the same organism. The results of both clinical studies and studies in an animal model of experimental vaginitis suggest that systemic CMI may not be the predominant host defense mechanism against C. albicans vaginal infections. Rather, locally acquired mucosal immunity, distinct from that in the peripheral circulation, is now under consideration as an important host defense at the vaginal mucosa, as well as the notion that changes in local CMI mechanism(s) may predispose to RVVC. PMID:8809464

  3. Systemic candidiasis: diagnosis from cutaneous manifestations.

    Slater, D N; Wylde, P; Harrington, C I; Worth, R

    1982-01-01

    Three patients are described who developed systemic candidiasis. Each had either leukaemia or lymphoma, and developed a similar erythematous maculopapular rash which, in places, was purpuric. In the first patient the nature of the rash was not appreciated during life, but postmortem histology revealed candida within the lesions. In the other two patients, a diagnosis of systemic candidiasis was successfully established by skin biopsy. This paper emphasizes that a maculopapular rash can be rel...

  4. Clinical and microbiological diagnosis of oral candidiasis

    Coronado Castellote, Laura; Jiménez Soriano, Yolanda

    2013-01-01

    Introduction: Candidiasis or oral candidiasis is the most frequent mucocutaneous mycosis of the oral cavity. It is produced by the genus Candida, which is found in the oral cavity of 53% of the general population as a common commensal organism. One hundred and fifty species have been isolated in the oral cavity, and 80% of the isolates correspond to Candida albicans, which can colonize the oral cavity alone or in combination with other species. Transformation from commensal organism to pathog...

  5. VULVO VAGINAL CANDIDIASIS : IMPORTANCE OF SPECIES IDENTIFICATION

    Swarajya Lakshmi

    2014-01-01

    Full Text Available OBJECTIVES : Vulvo Vaginal Candidiasis is a common nagging problem faced by 75% of women in reproductive age group. Present study was undertaken to determine the prevalence of Candida in patients suffering from vaginitis , to assess predisposing factors and correlate the symptoms with gram stain for presumptive diagnosis of Candidiasis. METHODS : A prospective study of the laboratory diagnosis of vulvovaginal candidiasis (VVC was carried out in 100 women presenting with symptoms suggestive of vaginosis in the reproductive age group. Investigation s included microscopy and culture for yeast. Candida is identified, based on growth on SDA, corn meal agar and Saba raud’s Triphenyl tetrazolium agar, and assimilation and fermentation of sugars. RESULTS : Candida was isolated in 33% of women. Clue cells on gram stain suggestive of bacterial vaginosis was seen in equal number of women, whereas mixed infection was found in 9%. Candida albicans accounted for 15% and nonalbicans species for 85% . O f the non albicans species, Candida glabrata was the commonest (4 2%. Pruritus with or without vaginal discharge and vaginal erythema were the most common symptoms and signs in women with positive Candida culture. CONCLUSION : On comparing the significance of gram stain and culture for presumptive diagnosis of candidiasi s, culture was more significant than gram stain alone. In present study, the rate of culture positivity was 33% and C. glabrata was the predominant species. VVC cannot be diagnosed by clinical criteria alone and requires confirmation by culture including i dentification of species.

  6. New Model of Oropharyngeal Candidiasis in Mice

    Kamai, Yasuki; Kubota, Mikie; Kamai, Yoko; Hosokawa, Tsunemichi; Fukuoka, Takashi; Filler, Scott G.

    2001-01-01

    We established a straightforward murine model of oropharyngeal candidiasis. Mice were immunosuppressed with cortisone acetate, anesthetized, and then inoculated by placing cotton wool balls saturated with Candida albicans sublingually for 2 h. A prolonged, reproducible infection was induced. This model may be useful for antifungal screening or pathogenesis studies.

  7. Genotype comparisons of strains of Candida albicans from patients with cutaneous candidiasis and vaginal candidiasis

    SHE Xiao-dong; WANG Xue-jun; FU Mei-hua; SHEN Yong-nian; LIU Wei-da

    2008-01-01

    Background It is uncertain whether genotypes of Candida albicans (C. Albicans) are associated with colonizing body locations or variant conditions of infection. The aim of this study was to investigate whether there are significant associations between strain genotypes and body sites of infection and to determine the potential pathogenesis of cutaneous candidiasis at multiple locations.Methods A total of 151 strains of C. Albicans were isolated from 74 infant patients with cutaneous candidiasis and 61 female patients with vaginal candidiasis. Patients were grouped according to the body sites and underlying conditions of infection. Genolypes were identified by polymerase chain reaction (PCR) of the 25S rDNA and PCR-restriction fragment length polymorphism (RFLP) of ALT repeals digested with EcoRI and Clal.Results Ten genotypes were detected. There were significant differences in genotype frequencies between the two groups. However, we found no clear association between genotypes and the sites of cutaneous infection or the underlying conditions of vaginal candidiasis (VVC). In addition, strains of C. Albicans from multiple cutaneous locations of the same patient had identical genotypes.Conclusions Populations of C. Albicans from patients with cutaneous and vaginal candidiasis were genetically different. However, the lack of genetic difference between strains from different body sites with cutaneous infections or from different underlying conditions for VVC suggests no evidence of genotype selection for different skin surfaces or patients with different underlying conditions for VVC.

  8. Current treatment of oral candidiasis: A literature review

    Garcia Cuesta, Carla; Sarrión Pérez, María Gracia; Bagán Sebastián, José Vicente

    2014-01-01

    Candidiasis or oral candidosis is one of the most common human opportunistic fungal infections of the oral cavity. This pathology has a wide variety of treatment which has been studied until these days. The present study offers a literature review on the treatment of oral candidiasis, with the purpose of establish which treatment is the most suitable in each case. Searching the 24 latest articles about treatment of candidiasis it concluded that the incidence depends on the type of the candidi...

  9. What Makes Oral Candidiasis Recurrent Infection? A Clinical View

    Darwazeh, Azmi M. G.; Darwazeh, Tamer A.

    2014-01-01

    Clinical oral Candida infection (candidiasis) is one of the common oral mucosal infections, and its management is usually frustrating due to either treatment failure or recurrence. Historically, oral candidiasis has been branded as disease of diseased. The unsuccessful management of oral candidiasis can due to either incorrect diagnosis, failure to identify (or correct) the underlying predisposing factor(s), or inaccurate prescription of antifungal agents. Failure to properly treat oral ca...

  10. Esophageal candidiasis among a dyspeptic population

    Al Mofleh Ibrahim

    1999-01-01

    Full Text Available This is a retrospective study of 59 patients endoscoped over a period of six years at a private clinic and were found to have esophageal candidiasis. The median age was 46.38 years. Thirty (51 % patients had no precipitating factors. Only 18 (30% patients had typical symptoms. The distal part of the esophagus was more often involved. The thrush was scattered in 57 (97% patients. The endoscopic finding was confirmed by cytology in all patients included. Symptoms improved on oral nystatin treatment and simultaneous treatment of associated conditions in all 46 patients followed up. In conclusion, esophageal candidiasis appear to be not uncommon among dyspeptic population. It presents more frequently with atypical symptoms and responds well to oral nystatin treatment.

  11. Probiotics as Antifungals in Mucosal Candidiasis.

    Matsubara, Victor H; Bandara, H M H N; Mayer, Marcia P A; Samaranayake, Lakshman P

    2016-05-01

    Candidais an opportunistic pathogen that causes mucosal and deep systemic candidiasis. The emergence of drug resistance and the side effects of currently available antifungals have restricted their use as long-term prophylactic agents for candidal infections. Given this scenario, probiotics have been suggested as a useful alternative for the management of candidiasis. We analyzed the available data on the efficacy of probiotics in candidal colonization of host surfaces. A number of well-controlled studies indicate that probiotics, particularly lactobacilli, suppressCandidagrowth and biofilm development in vitro.A few clinical trials have also shown the beneficial effects of probiotics in reducing oral, vaginal, and enteric colonization byCandida; alleviation of clinical signs and symptoms; and, in some cases, reducing the incidence of invasive fungal infection in critically ill patients. Probiotics may serve in the future as a worthy ally in the battle against chronic mucosal candidal infections. PMID:26826375

  12. Radiological findings in the diagnosis of genitourinary candidiasis

    The presence of fungus balls within the collecting system is an important clue to the radiological diagnosis of genitourinary candidiasis. In this report, an 8-month-old infant with this opportunistic infection is described. Emphasis is placed on the radiological findings of renal candidiasis, including previously unreported MR appearances. Sonographic and Doppler findings of accompanying Candida epididymitis are also described. (orig.)

  13. Radiological findings in the diagnosis of genitourinary candidiasis

    Erden, A. [Dept. of Radiology, Univ. of Ankara (Turkey); Hafta Sokak, Ankara (Turkey); Fitoz, S.; Karaguelle, T.; Tuekel, S.; Akyar, S. [Dept. of Radiology, Univ. of Ankara (Turkey)

    2000-12-01

    The presence of fungus balls within the collecting system is an important clue to the radiological diagnosis of genitourinary candidiasis. In this report, an 8-month-old infant with this opportunistic infection is described. Emphasis is placed on the radiological findings of renal candidiasis, including previously unreported MR appearances. Sonographic and Doppler findings of accompanying Candida epididymitis are also described. (orig.)

  14. Congenital candidiasis presenting as septic shock without rash

    Carmo, Kathryn Browning; Evans, Nick; Isaacs, David

    2009-01-01

    Congenital candidiasis is rare and often benign. This report describes the case of twins born at 32 weeks of gestation with different manifestations of congenital candidiasis. One twin was born well though neutropenic, and died from overwhelming sepsis with septic shock at 22 h. The other twin presented with a delayed onset of rash at 2 days, remained well and survived.

  15. Candidiasis oral en el paciente mayor

    E. Otero Rey; M. Peñamaría Mallón; M. Rodríguez Piñón; B. Martín Biedma; A. Blanco Carrión

    2015-01-01

    La candidiasis o candidosis oral es la enfermedad infecciosa ocasionada por el crecimiento de las colonias de Cándida y la penetración de las mismas en los tejidos orales cuando las barreras físicas y las defensas del huésped se encuentran alteradas. Es una infección frecuente de la cavidad oral de los adultos de edad avanzada. Aunque la incidencia real se desconoce, se sabe que existe una prevalencia aumentada en ciertas ocasiones como ocurre en ancianos, en presencia de prótesis mucosoporta...

  16. A child with autoimmune polyendocrinopathy candidiasis and ectodermal dysplasia treated with immunosuppression: a case report

    O'Gorman, Clodagh S; Shulman, Rayzel M; Lara-Corrales, Irene; POPE, ELENA; Marcon, Margaret; Grasemann, Hartmut; Schneider, Rayfel; Upton, Julia; Sochett, Etienne B.; Kolfin, Dror; Cohen, Eyal

    2013-01-01

    Introduction: Common features of autoimmune polyendocrinopathy-candidiasis-ectodermal dysplasia include candidiasis, hypoparathyroidism and hypoadrenalism. The initial manifestation of autoimmune polyendocrinopathy-candidiasis-ectodermal dysplasia may be autoimmune hepatitis, keratoconjunctivitis, frequent fever with or without a rash, chronic diarrhea, or different combinations of these with or without oral candidiasis. Case presentation: We discuss a profoundly affected 2.9-y...

  17. Experimental Models of Vaginal Candidiasis and Their Relevance to Human Candidiasis.

    Cassone, Antonio; Sobel, Jack D

    2016-05-01

    Vulvovaginal candidiasis (VVC) is a high-incidence disease seriously affecting the quality of life of women worldwide, particularly in its chronic, recurrent forms (RVVC), and with no definitive cure or preventive measure. Experimental studies in currently used rat and mouse models of vaginal candidiasis have generated a large mass of data on pathogenicity determinants and inflammation and immune responses of potential importance for the control of human pathology. However, reflection is necessary about the relevance of these rodent models to RVVC. Here we examine the chemical, biochemical, and biological factors that determine or contrast the forms of the disease in rodent models and in women and highlight the differences between them. We also appeal for approaches to improve or replace the current models in order to enhance their relevance to human infection. PMID:26883592

  18. Candidiasis oral en el paciente mayor

    E. Otero Rey

    2015-06-01

    Full Text Available La candidiasis o candidosis oral es la enfermedad infecciosa ocasionada por el crecimiento de las colonias de Cándida y la penetración de las mismas en los tejidos orales cuando las barreras físicas y las defensas del huésped se encuentran alteradas. Es una infección frecuente de la cavidad oral de los adultos de edad avanzada. Aunque la incidencia real se desconoce, se sabe que existe una prevalencia aumentada en ciertas ocasiones como ocurre en ancianos, en presencia de prótesis mucosoportadas, xerostomía o en patologías asociadas frecuentemente en los mayores. Los tipos clínicos más característicos son la forma seudomembranosa y la eritematosa (palatina y lingual. Pueden tener evolución aguda o crónica según la persistencia de los factores predisponentes. También son frecuentes procesos bucales comúnmente asociados: estomatitis protética, queilitis angular, glositis romboidal y lengua vellosa. La mayor parte de las candidiasis orales tienen un diagnóstico clínico, pero ha de confirmarse demostrando la penetración de la cándida en la mucosa oral, siendo el frotis la técnica de elección. Antes de comenzar el tratamiento, debemos estar seguros que se trata de una candidiasis oral, el tipo clínico y los factores predisponentes relacionados con la infección. Empezaremos siempre eliminando estos factores predisponentes, en el adulto mayor, la polifarmacología, la xerostomía, enfermedades crónicas y el uso de prótesis mucosoportadas son situaciones frecuentes que habrá que controlar. Instauraremos medidas higiénicas bucales y posteriormente si es necesario, utilizaremos fármacos antifúngicos, comenzando siempre con formas tópicas.

  19. Treatment options for chronic mucocutaneous candidiasis.

    van de Veerdonk, Frank L; Netea, Mihai G

    2016-07-01

    Autosomal dominant chronic mucocutaneous candidiasis (AD-CMC) is a rare and severe primary immunodeficiency that is characterized by mucocutaneous fungal infection, autoimmunity, cerebral aneurysms, and oropharyngeal and esophageal cancer. Recently, it was discovered that STAT1 mutations are responsible for AD-CMC. These mutations lead to the inability of STAT1 to be dephosphorylated, resulting in hyperphosphorylation, increased binding to the DNA, and gain of function (GOF) effects on STAT1 signaling. Furthermore, a characteristic feature of AD-CMC patients is deficiency in the T-helper 17 (Th17) responses, which is believed to be the immunological cause of the mucocutaneous fungal infection. No targeted treatment other than lifelong antifungal prophylaxis exists for AD-CMC. However, the discovery of the genetic and immunological defects makes it now possible to explore new treatment strategies. This review will discuss immunomodulatory treatment options that can be explored in patients with STAT1 GOF mutations. PMID:27161991

  20. Congenital cutaneous candidiasis: A rare and unpredictable disease

    Sujit A Jagtap

    2011-01-01

    Full Text Available Congenital cutaneous candidiasis (CCC is an extremely rare disorder that presents within the first 6 days of life. The manifestations ranges from diffuse skin eruption without any systemic symptoms to respiratory distress, hepatosplenomegaly, sepsis, and death. We report a neonate who presented with generalized skin eruptions at birth, characterized by erythematous macules and papules. The eruption involved head, face, neck, trunk, and extremities. Candida albicans was demonstrated on direct KOH smear, skin biopsy. The disease implies a congenital intrauterine infection and is different from neonatal candidiasis, which manifests as thrush or diaper dermatitis. The infection is acquired from the maternal genital tract in an ascending fashion. Clinical features, direct smear examination of specimen, and appropriate cultures are useful in differentiating the lesions from other more common dermatoses of the neonatal period. Topical antifungal therapy is sufficient unless systemic candidiasis is present. Prognosis for congenital cutaneous candidiasis is good.

  1. Symptomatic and asymptomatic candidiasis in a pediatric intensive care unit

    Arslankoylu Ali Ertug; Kuyucu Necdet; Yilmaz Berna; Erdogan Semra

    2011-01-01

    Abstract Introduction This study aimed to examine the incidence, epidemiology, and clinical characteristics of symptomatic and asymptomatic candidiasis in a pediatric intensive care unit (PICU), and to determine the risk factors associated with symptomatic candidiasis. Methods This retrospective study included 67 patients from a 7-bed PICU in a tertiary care hospital that had Candida-positive cultures between April 2007 and July 2009. Demographic and clinical characteristics of the patients, ...

  2. Poly(I.C)-induced interferons enhance susceptibility of SCID mice to systemic candidiasis.

    J. Jensen; Vazquez-Torres, A; Balish, E

    1992-01-01

    In the absence of any demonstrable T- or B-cell responses, gnotobiotic CB-17 SCID (severe combined immunodeficient) mice not only show innate resistance to acute systemic (intravenous challenge) candidiasis but also manifest innate resistance to systemic candidiasis of endogenous (gastrointestinal tract) origin. Poly(I. C), a potent inducer of interferons (IFNs) in vivo, enhanced the susceptibility of CB-17 SCID mice to acute systemic candidiasis and to systemic candidiasis of endogenous orig...

  3. Host responses to Candida albicans: Th17 cells and mucosal candidiasis

    Conti, Heather R.; Gaffen, Sarah L

    2010-01-01

    Candida albicans causes mucosal and disseminated candidiasis, which represent serious problems for the rapidly expanding immunocompromised population. Until recently, Th1-mediated immunity was thought to confer the primary protection, particularly for oral candidiasis. However, emerging data indicate that the newly-defined Th17 compartment appears to play the predominant role in mucosal candidiasis.

  4. Disparate Requirement for T Cells in Resistance to Mucosal and Acute Systemic Candidiasis

    Jones-Carson, Jessica; Vazquez-Torres, Andres; Warner, Thomas; Balish, Edward

    2000-01-01

    Although highly susceptible to orogastric candidiasis, T-cell receptor δ- and α-chain knockout mice, deficient in γδ and αβ T cells, respectively, were found to be resistant to disseminated candidiasis of endogenous origin and to acute systemic candidiasis (resulting from intravenous injection).

  5. Two-phase treatment of inflamatory candidiasis of vulva

    K. Drljević

    2006-02-01

    Full Text Available The purpose of this prospective study was to examine successfulnes of applaying two-phase treatment of vulvar inflamatory candidiasis. At the initial phase antimycotic izokonazol 1% nitrate in combination with corticosteriod diflucorton valerate (Travocort, Schering AG, Germany was localy applied, and then pure antimycotic izoconasol nitrate (Travogen, Schering AG, Germany. Parametars that have been analyzed in the first phase of treatment were regression of inflamatory changes of vulva, and in the second phase healing of fungal disease. Two-phase treatment of vulvar inflamatory candidiasis showed full therapy benefit at cured patients. The initial short-term local application of combination corticosteroid and antimycotic showed almost a hundred percent regression of inflamatory changes and simptoms of vulvar inflamatory candidiasis, without any side-effects noted.

  6. Diagnostic Methods for Detection of Blood-Borne Candidiasis.

    Clancy, Cornelius J; Nguyen, M Hong

    2016-01-01

    β-D-glucan (Fungitell) and polymerase chain reaction-based (T2Candida) assays of blood samples are FDA-approved adjuncts to cultures for diagnosing candidemia and other types of invasive candidiasis, but their clinical roles are unclear. In this chapter, we describe laboratory protocols for performing Fungitell and T2Candida assays. We then discuss step-by-step methods for interpreting test results at the bedside using a Bayesian framework, and for incorporating assays into rational patient management strategies. Prior to interpreting results, clinicians must recognize that test performance varies based on the type of invasive candidiasis being diagnosed. In general, the type of invasive candidiasis that is most likely in a given patient can be identified, and the pretest likelihood of disease estimated. From there, positive and negative predictive values (PPV, NPV) for an assay can be calculated. At a population level, tests can be incorporated into screening strategies for antifungal treatment. NPV and PPV thresholds can be defined for discontinuing antifungal prophylaxis or initiating preemptive treatment, respectively. Using the thresholds, it is possible to assign windows of pretest likelihood for invasive candidiasis (and corresponding patient populations) in which tests are most likely to valuable. At the individual patient level, tests may be useful outside of the windows proposed for screening populations. The interpretive and clinical decision-making processes we discuss will be applicable to other diagnostic assays as they enter the clinic, and to existing assays as more data emerge from various populations. PMID:26519076

  7. Immunopathogenesis of oropharyngeal candidiasis in human immunodeficiency virus infection.

    de Repentigny, Louis; Lewandowski, Daniel; Jolicoeur, Paul

    2004-10-01

    Oropharyngeal and esophageal candidiases remain significant causes of morbidity in human immunodeficiency virus (HIV)-infected patients, despite the dramatic ability of antiretroviral therapy to reconstitute immunity. Notable advances have been achieved in understanding, at the molecular level, the relationships between the progression of HIV infection, the acquisition, maintenance, and clonality of oral candidal populations, and the emergence of antifungal resistance. However, the critical immunological defects which are responsible for the onset and maintenance of mucosal candidiasis in patients with HIV infection have not been elucidated. The devastating impact of HIV infection on mucosal Langerhans' cell and CD4(+) cell populations is most probably central to the pathogenesis of mucosal candidiasis in HIV-infected patients. However, these defects may be partly compensated by preserved host defense mechanisms (calprotectin, keratinocytes, CD8(+) T cells, and phagocytes) which, individually or together, may limit Candida albicans proliferation to the superficial mucosa. The availability of CD4C/HIV transgenic mice expressing HIV-1 in immune cells has provided the opportunity to devise a novel model of mucosal candidiasis that closely mimics the clinical and pathological features of candidal infection in human HIV infection. These transgenic mice allow, for the first time, a precise cause-and-effect analysis of the immunopathogenesis of mucosal candidiasis in HIV infection under controlled conditions in a small laboratory animal. PMID:15489345

  8. Thallium-201 accumulation in cerebral candidiasis: Unexpected finding on SPECT

    Tonami, N.; Matsuda, H.; Ooba, H.; Yokoyama, K.; Hisada, K.; Ikeda, K.; Yamashita, J. (Kanazawa Univ. (Japan))

    1990-06-01

    The authors present an unexpected finding of Tl-201 uptake in the intracerebral lesions due to candidiasis. SPECT demonstrated the extent of the lesions and a high target-to-background ratio. The regions where abnormal Tl-201 accumulation was seen were nearly consistent with CT scans of those enhanced by a contrast agent. After treatment, most of the abnormal Tl-201 accumulation disappeared.

  9. Ultrasound scan in the diagnosis of neonatal renal candidiasis

    To describe the most pertinent echographic findings regarding systemic and renal candidiasis in high-risk neonates. Echographic findings and clinical histories of 40 neonates in the neonatal intensive care unit were retrospectively analyzed. Thirty-eight presented systemic candidiasis with renal participation, while two showed only renal candidiasis. Ultrasound scans were performed using 7.5 MHz probes. Alterations in renal echo structure, presence of echogenic material without acoustic shadowing in the excretory system (mycetoma), presence of lithiasis, pyonephrosis and associated renal malformations were all evaluated. Ten patients presented renal alterations in ultrasound scan. Six children had originally shown increased eye-catching in the renal parenchyma which was resolved after medical treatment. Four children presented renal mycetoma, and in two there were renal malformations. Both of these exhibited a profile for pyonephrosis. One patient with renal mycetoma without urological abnormalities developed a lithiasis. Surgical intervention was unnecessary in all cases. The most common echographic findings in immature high-risk low-weight patients with systemic and renal candidiasis were alterations in the eye-catching of renal parenchyma and the presence of mycetoma. (Author) 22 refs

  10. Thallium-201 accumulation in cerebral candidiasis: Unexpected finding on SPECT

    The authors present an unexpected finding of Tl-201 uptake in the intracerebral lesions due to candidiasis. SPECT demonstrated the extent of the lesions and a high target-to-background ratio. The regions where abnormal Tl-201 accumulation was seen were nearly consistent with CT scans of those enhanced by a contrast agent. After treatment, most of the abnormal Tl-201 accumulation disappeared

  11. The "Other" Venereal Diseases: Herpes Simplex, Trichomoniasis and Candidiasis.

    McNab, Warren L.

    1979-01-01

    Although the term venereal disease has been synonymous with gonorrhea and syphilis, the Center for Disease Control now states that the number of new cases of herpes simplex, trichomoniasis, and candidiasis is rapidly approaching the number of cases of syphilis and gonorrhea. (MM)

  12. Ultrasound scan in the diagnosis of neonatal renal candidiasis; Ecografia en el diagnostico de la candidiasis renal neonatal

    Muro, D.; Sanguesa, C.; Torres, D.; Berbel, O.; Andres, V. [Hospital Infantil La Fe. Valencia (Spain)

    2003-07-01

    To describe the most pertinent echographic findings regarding systemic and renal candidiasis in high-risk neonates. Echographic findings and clinical histories of 40 neonates in the neonatal intensive care unit were retrospectively analyzed. Thirty-eight presented systemic candidiasis with renal participation, while two showed only renal candidiasis. Ultrasound scans were performed using 7.5 MHz probes. Alterations in renal echo structure, presence of echogenic material without acoustic shadowing in the excretory system (mycetoma), presence of lithiasis, pyonephrosis and associated renal malformations were all evaluated. Ten patients presented renal alterations in ultrasound scan. Six children had originally shown increased eye-catching in the renal parenchyma which was resolved after medical treatment. Four children presented renal mycetoma, and in two there were renal malformations. Both of these exhibited a profile for pyonephrosis. One patient with renal mycetoma without urological abnormalities developed a lithiasis. Surgical intervention was unnecessary in all cases. The most common echographic findings in immature high-risk low-weight patients with systemic and renal candidiasis were alterations in the eye-catching of renal parenchyma and the presence of mycetoma. (Author) 22 refs.

  13. Anti-fungal resistance in candida isolated from oral and diaper rash candidiasis in neonates

    Mohamadi, Jasem; Motaghi, Mahsa; Panahi, Jafar; Havasian, Mohamad Reza; Delpisheh, Ali; Azizian, Mitra; PAKZAD, Iraj

    2014-01-01

    The purpose of the present study is to evaluate the sensitivity of Candida species isolated from oral candidiasis and diaper dermatitis infections in children. The children referring to private and public clinics in Ilam, Iran were exmined for oral candidiasis and diaper dermatitis. In this study, 248 oral candidiasis and diaper dermatitis samples were collected and cultured.Candida species were identified by using standard methods. Resistance and sensitivity to amphotericin B, nystatin, keto...

  14. New Approaches in the Development of a Vaccine for Mucosal Candidiasis: Progress and Challenges

    AnnaVecchiarelli

    2012-01-01

    The commensal fungus Candida albicans causes mucosal candidiasis in the rapidly expanding number of immunocompromised patients. Mucosal candidiasis includes orapharyngeal, esophageal, gastrointestinal, and vaginal infections. Vulvovaginal candidiasis (VVC) and antimycotic-refractory recurrent VVC is a frequent problem in healthy child-bearing women. Both these mucosal infections can affect the quality of life and finding new therapeutical and preventive approaches is a challenge. A vaccine ag...

  15. Evaluating diagnosis and treatment of oral and esophageal candidiasis in Ugandan AIDS patients.

    Ravera, M.; Reggiori, A.; Agliata, A. M.; Rocco, R. P.

    1999-01-01

    A randomized cross-over clinical and endoscopic evaluation of 85 Ugandan patients showed that esophageal candidiasis in AIDS patients with oral candidiasis could be managed without endoscopy and biopsies. Oral lesions, especially when accompanied by esophageal symptoms, were sufficient for diagnosis. Miconazole was more effective than nystatin in treating esophageal candidiasis and could be a valid alternative to more expensive azolic drugs in developing countries.

  16. Highly-cited estimates of the cumulative incidence and recurrence of vulvovaginal candidiasis are inadequately documented

    Rathod, SD; Buffler, PA

    2014-01-01

    BACKGROUND Available literature concerning the epidemiologic or clinical features of vulvovaginal candidiasis commonly reports that: 75% of women will experience an episode of vulvovaginal candidiasis in their lifetimes, 50% of whom will experience at least a second episode, and 5-10% of all women will experience recurrent vulvovaginal candidiasis (≥4 episodes/1 year). In this debate we traced the three commonly cited statistics to their presumed origins. DISCUSSION It is apparent that t...

  17. Successful Management of an Extremely Premature Infant with Congenital Candidiasis

    Sota Iwatani

    2014-05-01

    Full Text Available Congenital candidiasis, which presents with a variety of clinical symptoms, is very rare in both term and preterm infants, and less than 100 neonatal cases have been reported in the medical literature. We describe the case of an extremely premature infant with congenital candidiasis, who was successfully treated and survived without major sequelae. A male infant was born at 25 weeks' gestation (weight, 834 g. He exhibited diffuse erythematous papules. Samples of his skin, pharyngeal mucus, gastric fluid, and tracheal aspirate were found to be Candida albicans—positive while blood cultures were negative. Further histopathological examinations revealed that Candida albicans mycelia had invaded the umbilical cord. After prompt antifungal therapy, the patient's skin lesions improved markedly, and he was discharged from hospital without any major complications. This report highlights the importance of characteristic skin lesions for the early diagnosis of Candida infections, especially in extremely premature infants.

  18. Oral candidiasis-adhesion of non-albicans Candida species

    Bokor-Bratić Marija B.

    2008-01-01

    Full Text Available Oral candidiasis is an opportunistic infection caused primarily by Candida albicans. However, in recent years, species of non-albicans Candida have been implicated more frequently in mucosal infection. Candida species usually reside as commensal organisms and are part of normal oral microflora. Determining exactly how transformation from commensal to pathogen takes place and how it can be prevented is continuous challenge for clinical doctors. Candidal adherence to mucosal surfaces is considered as a critical initial step in the pathogenesis of oral candidiasis. Acrylic dentures, acting as reservoirs, play an important role in increasing the risk from Candida colonisation. Thus, this review discusses what is currently known about the adhesion of non-albicans Candida species of oral origin to buccal epithelial cells and denture acrylics.

  19. Conventional and alternative antifungal therapies to oral candidiasis

    Paula Cristina Anibal

    2010-12-01

    Full Text Available Candida-associated denture stomatitis is the most common form of oral candidal infection, with Candida albicans being the principal etiological agent. Candida adheres directly or via an intermediary layer of plaque-forming bacteria to denture acrylic. Despite antifungal therapy to treat denture stomatitis, infection is reestablished soon after the treatment ceases. In addition, many predisposing factors have been identified as important in the development of oral candidiasis, including malnourishment, common endocrine disorders, such as diabetis mellitus, antibacterial drug therapy, corticosteroids, radiotherapy and other immunocompromised conditions, such as acquired immunodeficiency syndrome (AIDS. These often results in increased tolerance to the most commonly used antifungals. So this review suggests new therapies to oral candidiasis.

  20. Pharmacodynamics of Fluconazole in a Murine Model of Systemic Candidiasis

    Louie, Arnold; Drusano, George L.; Banerjee, Partha; Liu, Qing-Feng; Liu, Weiguo; Kaw, Pamela; Shayegani, Mehdi; Taber, Harry; Miller, Michael H.

    1998-01-01

    In this study we defined the pharmacodynamic parameter that optimizes outcome in deep-seated Candida albicans infections treated with fluconazole. Using a murine model of systemic candidiasis, we conducted single-dose dose-ranging studies with fluconazole to determine the dosage of this drug that resulted in a 50% reduction in fungal densities (50% effective dose [ED50]) in kidneys versus the fungal densities in the kidneys of untreated controls. We found that the ED50 of fluconazole given in...

  1. Efficiency of fenticonazole for the treatment of vaginal candidiasis

    Živaljević Biljana

    2012-01-01

    Full Text Available Introduction. Uncomplicated vulvovaginal candidiasis appears in 75% women of reproductive age. The most frequent causes are Candida albicans (85-95% or C. glabrata, and infrequently C. krusei, C. tropicalis, C. parapsilosis, C. pseudotropicalis, etc. Objective. The aim of the study was to investigate efficiency and safety of fenticonazole for vaginal candidiasis treatment. Methods. Therapeutic effect of a single 600 mg fenticonasole vaginal capsule was observed in 417 women, aged 16-67, in five centers in Serbia. In all women, before the treatment, vaginal candidiasis was confirmed by testing of vaginal smear. Based on smear findings and associated symptoms observed on the 7th and 28th day after therapy administration, treatment results were evaluated. On the next day after drug application the patients recorded by using a questionnaire their own feelings on withdrawal symptoms and possible side effects in the period prior to the first control. Results. Control after seven days showed a statistically significant decrease of symptoms. In 385 women, vaginal smear was found negative to yeast and yeast blastospores. Within the first seven days after treatment 84 women had to repeat therapy due to the persistence of symptoms or positive vaginal smear. After 28 days we recorded full recovery in 392 patients, clinical improvement in eight, no change in 16, and deterioration in one patient only. Side effects were very seldom, mostly in the form of a slight redness of the vulva and vagina, and mild itching during several days. Conclusion. Our observations confirmed good efficacy and safety of fenticonazole in the treatment of vaginal candidiasis.

  2. Oral candidiasis-adhesion of non-albicans Candida species

    Bokor-Bratić Marija B.

    2008-01-01

    Oral candidiasis is an opportunistic infection caused primarily by Candida albicans. However, in recent years, species of non-albicans Candida have been implicated more frequently in mucosal infection. Candida species usually reside as commensal organisms and are part of normal oral microflora. Determining exactly how transformation from commensal to pathogen takes place and how it can be prevented is continuous challenge for clinical doctors. Candidal adherence to mucosal surfaces is conside...

  3. Insights from human studies into the host defense against candidiasis

    Filler, Scott G

    2011-01-01

    Candida spp. are the most common cause of mucosal and disseminated fungal infections in humans. Studies using mutant strains of mice have provided initial information about the roles of dectin-1, CARD9, and Th17 cytokines in the host defense against candidiasis. Recent technological advances have resulted in the identification of mutations in specific genes that predispose humans to develop candidal infection. The analysis of individuals with these mutations demonstrates that dectin-1 is crit...

  4. Systemic vs. Topical Therapy for the Treatment of Vulvovaginal Candidiasis

    Sebastian Faro

    1994-01-01

    It is estimated that 75% of all women will experience at least 1 episode of vulvovaginal candidiasis (VVC) during their lifetimes. Most patients with acute VVC can be treated with short-term regimens that optimize compliance. Since current topical and oral antifungals have shown comparably high efficacy rates, other issues should be considered in determining the most appropriate therapy. It is possible that the use of short-durat...

  5. Invasive Esophageal Candidiasis with Chronic Mediastinal Abscess and Fatal Pneumomediastinum.

    Aghdam, Mohammad Reza F; Sund, Ståle

    2016-01-01

    BACKGROUND Invasive candidiasis is a potential problem for patients receiving long-term immunosuppressive treatment. Psoriatic arthritis is one of many chronic diseases that can be successfully treated with immunosuppressive drugs, in spite of a documented and accepted risk for infectious complications. Critical awareness of possible infection must be part of the surveillance of such patients. CASE REPORT This is the case of a 68-year-old Norwegian male, treated with long-term immunosuppression for psoriatic arthritis, hospitalized with acute subcutaneous and mediastinal emphysema of unknown cause. He died of acute respiratory failure with circulatory collapse shortly after admission. The autopsy revealed mediastinal and subcutaneous emphysema and a mediastinal abscess containing Candida with probable entrance from the esophagus. CONCLUSIONS We consider invasive candidiasis of the esophagus to be the cause of both the chronic abscess and the acute mediastinal emphysema. This case illustrates the importance of awareness of invasive candidiasis as a possible complication in a patient with long-term immunosuppression. PMID:27389822

  6. PCR monitoring of response to liposomal amphotericin B treatment of systemic candidiasis in neutropenic mice

    A.J.M. Munting-van Deventer; W.H.F. Goessens (Wil); A.F. van Belkum (Alex); E.W.M. van Etten (Els); H.J. van Vliet; H.A. Verbrugh (Henri)

    1996-01-01

    textabstractWhen a diagnosis of invasive candidiasis has been made, treatment with toxic fungicidal agents is inevitable. The crucial decision of when to stop such treatment is difficult to make, because cultures are often negative despite ongoing invasive candidiasis and can there

  7. Optimal management of oropharyngeal and esophageal candidiasis in patients living with HIV infection

    Jose A Vazquez

    2010-04-01

    Full Text Available Jose A VazquezDivision of Infectious Diseases, Henry Ford Hospital, Wayne State University School of Medicine, Detroit, MI, USAAbstract: Mucocutaneous candidiasis is frequently one of the f irst signs of human immunodeficiency virus (HIV infection. Over 90% of patients with AIDS will develop oropharyngeal candidiasis (OPC at some time during their illness. Although numerous antifungal agents are available, azoles, both topical (clotrimazole and systemic (fluconazole, itraconazole, voriconazole, posaconazole have replaced older topical antifungals (gentian violet and nystatin in the management of oropharyngeal candidiasis in these patients. The systemic azoles, are generally safe and effective agents in HIV-infected patients with oropharyngeal candidiasis. A constant concern in these patients is relapse, which is dependent on the degree of immunosuppression commonly seen after topical therapy, rather than with systemic azole therapy. Candida esophagitis (CE is also an important concern since it occurs in more than 10% of patients with AIDS and can lead to a decrease in oral intake and associated weight loss. Fluconazole has become the most widely used antifungal in the management of mucosal candidiasis. However, itraconazole and posaconazole have similar clinical response rates as fluconazole and are also effective alternative agents. In patients with fluconazole-refractory mucosal candidiasis, treatment options now include itraconazole solution, voriconazole, posaconazole, and the newer echinocandins (caspofungin, micafungin, and anidulafungin.Keywords: oropharyngeal candidiasis, esophageal candidiasis, HAART, antifungal agents, HIV, AIDS

  8. Adjuvant corticosteroid therapy in hepatosplenic candidiasis-related IRIS

    Cengiz Bayram

    2012-01-01

    Full Text Available

    Hepatosplenic candidiasis (HSC is a form of invasive fungal infection that occurs most commonly in patients with acute leukemia treated with chemotherapy and requires protracted antifungal therapy. Immune reconstitution inflammatory syndrome (IRIS is best characterized as a dysregulated inflammatory responses triggered by rapid resolution of immunosuppression.

    We present a child diagnosed with standard-risk precursor B cell-acute lymphoblastic leukemia who developed HSC and Candida-related IRIS during recovery of neutropenia associated with induction chemotherapy. Addition of corticosteroid therapy to antifungal treatment is associated with the resolution of the clinical symptoms and laboratory findings

  9. PRIMARY LARYNGEAL CANDIDIASIS WITH TUBERCULOSIS MIM ICKING LARYNGEAL NEOPLASM

    Aditya Prakash

    2012-11-01

    Full Text Available ABSTRACT: A 50 -year-old man presented with hoarseness of voic e, cough with expectoration for one month and severe dyspnoea from 15 days. C T Scan of the neck revealed soft tissue lesion circumferentially involving supra-glottic reg ion with effacement of bilateral pyriform fossa predominantly on left side. Extralaryngeal spre ad to adjacent paralaryngeal space was also seen on left side. Marked airway luminal compro mise was seen at the level of false vocal cord . Primary diagnosis of laryngeal malignancy w as considered on findings revealed by CT scan neck. Direct laryngoscopy revealed erythema and ulcer with white pus discharge on left false vocal cord. A swab was taken from ulcer on left false vocal cord and sent for microbiological examination. The microbiological fi ndings confirmed Mycobacterium tuberculosis with Candida albicans.. CONCLUSION: Primary candidiasis with tuberculosis in the laryngeal mucosa is a rare entity. The clinical and pathological presentations of laryng eal candidiasis and tuberculosis might be confused with those for malignant lesions if extr alaryngeal spread is seen. Potential pitfalls in diagnosis and the importance of microbiological exam ination in rare case of dual superimposed primary laryngeal infections has been outlined

  10. Invasive candidiasis: from mycobiome to infection, therapy, and prevention.

    Lagunes, L; Rello, J

    2016-08-01

    Candida spp. are commonly found in humans, colonizing most healthy individuals. A high prevalence of invasive candidiasis has been reported in recent years. Here, we assess the relation between Candida spp. as part of the human mycobiome, the host defense mechanisms, and the pathophysiology of invasive disease in critically ill patients. Many hypotheses have been proposed to explain the different immune responses to the process where Candida goes through healthy mycobiome to colonization to invasion; the involvement of other microbiota inhabitants, changes in temperature, low nitrogen levels, and the caspase system activation have been described. Patients admitted to an intensive care unit (ICU) are at the highest risk for invasive candidiasis, mostly due to the severity of their disease, immune-suppressive states, prolonged length of stay, broad-spectrum antibiotics, septic shock, and Candida colonization. The first approach should be using predictive scores as screening, followed by the determination of biomarkers (when available), and, in the near future, probably immune-genomics and analysis of the clinical background in order to initiate prompt and correct treatment. Regarding treatment, the initiation with an echinocandin is strongly recommended in critically ill patients. In conclusion, prompt treatment and adequate source control in the more severe patients remains the ultimate goal, as well as restoration of a healthy microbiota. PMID:27146877

  11. Evaluation of effect of topical ozone therapy on salivary Candidal carriage in oral candidiasis

    Isha Khatri

    2015-01-01

    Results and Conclusion: There was gradual but significant reduction in Candidal CFU count in both groups. At the end of the treatment, Candidal CFU count reduction in ozone group (60.5% reduction was more than the clotrimazole group (32.3% reduction. 14 patients (70% with candidiasis in ozone group were reduced to 6 (30% whereas only 8 patients (40% out of 13 (65% in clotrimazole group, although intergroup comparison was not statistically significant. Ozone therapy was much more effective in reducing the patients with candidiasis to a state of carriers. These findings suggest that ozonated water might be useful to treat oral candidiasis.

  12. Prevalence and clinico-mycological profile of vulvovaginal candidiasis in a tertiary care hospital

    Rupal Samal

    2015-08-01

    Results: Among 125 cases studies, 62 positive cases for Candidiasis were reported with an approximate incidence of 50%. Further speciation identification showed C. albicans positivity in 45 cases and 17 cases for non albicans species. Women of second and third decade were predominantly affected by vulvovaginal candidiasis with abdominal pain and pruritis being a common clinical presentation. Conclusions: The prevalence of vulvovaginal candidiasis is on higher margin especially among reproductive age group. Clinical profile must be further correlated with laboratory data for speciation, thereby guiding in prompt and appropriate treatment modalities on best patient care. [Int J Reprod Contracept Obstet Gynecol 2015; 4(4.000: 1142-1147

  13. A radioimmunoassay method for the rapid detection of Candida antibodies is experimental systematic candidiasis

    Rabbits were employed as experimental models to evaluate a solid-phase radioimmunoassay (RIA) method for the diagnosis of systematic candidiasis. Ten rabbits were incubated subcutaneously to mimic superficial candidiasis and were found to produce no antibodies to Candida as determined by both immunodiffusion and RIA procedures. However, 94 per cent of 18 rabbits systematically infected by intravenous injection of Candida cells were observed to produce antibody as assessed by the RIA technique. These data encourage further tests with human sera and the continued development of this RIA procedure as a useful tool in the early serodiagnosis of systematic candidiasis. (Auth.)

  14. Treatment of vulvovaginal candidiasis: a review of the literature.

    Dovnik, Andraž; Golle, Andrej; Novak, Dušan; Arko, Darja; Takač, Iztok

    2015-01-01

    Vulvovaginal candidiasis (VVC) affects around three-quarters of all women during their reproductive age, although the exact incidence of VVC is difficult to determine because many patients are self-treated. The infections are divided into complicated and uncomplicated. Uncomplicated VVC is most effectively treated with local azoles. Oral treatment with a single dose of fluconazole is also effective for treating uncomplicated VVC. Treatment of complicated VVC is prolonged and most commonly consists of multiple doses of oral fluconazole or at least 1 week of local azoles. The role of probiotics in treating VVC is still disputed. This article presents a review of the literature on the various treatment options for VVC. Treatment for the most common pathogens that cause complicated VVC is also discussed. PMID:25770305

  15. Treatment of invasive candidiasis: between guidelines and daily clinical practice.

    Tagliaferri, Enrico; Menichetti, Francesco

    2015-06-01

    Invasive candidiasis, including candidemia (IC/C), is a major cause of morbidity and mortality among hospitalized patients. While incidence is higher in intensive care units, the majority of cases of candidemia are documented in medical wards. Although Candida albicans is still the most frequently isolated species, IC/C due to non-albicans species, usually less susceptible to fluconazole, is increasing. Early identification of patients at risk, knowledge of local epidemiology and prompt efforts to define etiologic diagnosis play a pivotal role for appropriateness. Starting therapy with an echinocandin, switching then to fluconazole when possible, seems to represent a potentially useful strategy for the management of IC/C. The choice between the three echinocandins should be based on the specific indications, pharmacokinetic/pharmacodynamic profile, clinical experience and relative cost. PMID:25818660

  16. Scintigraphy usefulness in the diagnosis of visceral candidiasis

    From the features of two cases, the authors stress the usefulness of the scintigraphy as regards to the diagnosis of visceral candidial abscesses. Such fungal localisations are not unfrequent, especially in immunodeficient patients (haematologic malignancies undergoing chemotherapy, lupus, serious visceral illness...). The positive diagnosis is uneasy because of non-specific clinical features and frequent negative blood cultures. Splenic localisation is the most likely. Citrate Gallium scintigraphy together with splenic labelled RBC scan enables us to give a precise view of the splenic involvment and even of the abdominal extension of the fungal abscess. From the literature review and these two cases, the excellent adequacy of the scintigraphy to the follow-up of systemic candidiasis is underlined and is compared to other usual morphological studies such as US scan and CT examination. In both cases, the diagnosis is fully confirmed by mycological examination

  17. Scintigraphy usefulness in the diagnosis of visceral candidiasis

    Ythier, H.; Legghe, R.; Foucher, C.

    1987-01-01

    From the features of two cases, the authors stress the usefulness of the scintigraphy as regards to the diagnosis of visceral candidial abscesses. Such fungal localisations are not unfrequent, especially in immunodeficient patients (haematologic malignancies undergoing chemotherapy, lupus, serious visceral illness...). The positive diagnosis is uneasy because of non-specific clinical features and frequent negative blood cultures. Splenic localisation is the most likely. Citrate Gallium scintigraphy together with splenic labelled RBC scan enables us to give a precise view of the splenic involvment and even of the abdominal extension of the fungal abscess. From the literature review and these two cases, the excellent adequacy of the scintigraphy to the follow-up of systemic candidiasis is underlined and is compared to other usual morphological studies such as US scan and CT examination. In both cases, the diagnosis is fully confirmed by mycological examination.

  18. Candidiasis of the liver and spleen in childhood

    Miller, J.H.; Greenfield, L.D.; Wald, B.R.

    1982-02-01

    Four children with acute leukemia and surgically documented candidiasis of the liver and/or spleen were examined with a combination of diagnostic imaging modalities including /sup 99/mTc-sulfur colloid and /sup 67/Ga-citrate scintigraphy, gray-scale ultrasound, and body computed tomography (CT). Abnormalities were detected in every individual examined. /sup 99/mTc-sulfur colloid scintigraphy revealed cold areas in the liver or spleen. With /sup 67/Ga scintigraphy, these areas were cold in some individuals and hot in others. Gray-scale ultrasound demonstrated hypoechoic lesions with central areas of increased echogenicity in hepatic involvement, and hypoechoic replacement of the spleen in splenic involvement. CT in one patient revealed low-density areas without contrast enhancement within the hepatic parenchyma and unsuspected renal involvement.

  19. Candidiasis of the liver and spleen in childhood

    Four children with acute leukemia and surgically documented candidiasis of the liver and/or spleen were examined with a combination of diagnostic imaging modalities including /sup 99m/Tc-sulfur colloid and /sup 67/Ga- citrate scintigraphy, gray-scale ultrasound, and body computed tomography (CT). Abnormalities were detected in every individual examined. /sup 99m/Tc-sulfur colloid scintigraphy revealed ''cold'' areas in the liver or spleen. With /sup 67/Ga scintigraphy, these areas were ''cold'' in some individuals and ''hot'' in others. Gray-scale ultrasound demonstrated hypoechoic lesions with central areas of increased echogenicity in hepatic involvement, and hypoechoic replacement of the spleen in splenic involvement. CT in one patient revealed low-density areas without contrast enhancement within the hepatic parenchyma and unsuspected renal involvement

  20. Candidiasis of the liver and spleen in childhood

    Miller, J.H. (Childrens Hospital of Los Angeles, CA); Greenfield, L.D.; Wald, B.R.

    1982-02-01

    Four children with acute leukemia and surgically documented candidiasis of the liver and/or spleen were examined with a combination of diagnostic imaging modalities including /sup 99m/Tc-sulfur colloid and /sup 67/Ga- citrate scintigraphy, gray-scale ultrasound, and body computed tomography (CT). Abnormalities were detected in every individual examined. /sup 99m/Tc-sulfur colloid scintigraphy revealed ''cold'' areas in the liver or spleen. With /sup 67/Ga scintigraphy, these areas were ''cold'' in some individuals and ''hot'' in others. Gray-scale ultrasound demonstrated hypoechoic lesions with central areas of increased echogenicity in hepatic involvement, and hypoechoic replacement of the spleen in splenic involvement. CT in one patient revealed low-density areas without contrast enhancement within the hepatic parenchyma and unsuspected renal involvement.

  1. Oropharyngeal Candidiasis in Palliative Care Patients in Denmark

    Astvad, Karen; Johansen, Helle Krogh; Høiby, Niels;

    2015-01-01

    BACKGROUND: Oropharyngeal candidiasis (OPC) is a significant cause of morbidity, especially among patients with advanced cancer. The incidence and significance of yeast carriage and OPC in the palliative care setting in Denmark is unknown. The best diagnostic strategy and treatment regimen has...... to be defined. OBJECTIVE: This study evaluated the clinical and microbiological incidence of yeast carriage/OPC and assessed available diagnostic procedures-culture and microscopy. The distribution of Candida species and fluconazole susceptibility was determined. METHODS: Terminal care patients admitted...... recently treated with azoles. CONCLUSIONS: In total, 52% of culture-positive patients harbored at least one isolate with innately or acquired decreased fluconazole susceptibility. Therefore, susceptibility testing appears recommendable for patients with clinical signs of OPC....

  2. Imaging of liver and spleen candidiasis in patients with acute leukemia

    Seino, Yasuo; Tamakawa, Y.; Kato, T.; Kimura, Y.; Miyazaki, S.; Miura, R.; Ishida, H.

    1988-01-01

    Four patients with acute leukemia were found to have candidal abscess of liver and spleen. CT and US showed hepatosplenomegaly and microabscess. These findings might be useful in diagnosis of visceral candidiasis.

  3. Imaging of liver and spleen candidiasis in patients with acute leukemia

    Four patients with acute leukemia were found to have candidal abscess of liver and spleen. CT and US showed hepatosplenomegaly and microabscess. These findings might be useful in diagnosis of visceral candidiasis. (author)

  4. Clinical assessment of oral mucositis and candidiasis compare to chemotherapic nadir in transplanted patients

    PATUSSI Cleverson; Sassi, Laurindo Moacir; MUNHOZ Eduardo Ciliao; ZANICOTTI Roberta Targa Stramandinoli; Schussel, Juliana Lucena

    2014-01-01

    Oral mucositis is a chief complication in patients undergoing hematopoietic stem cell transplantation (HSCT). It is considered a toxic inflammatory reaction that interferes with the patient’s recuperation and quality of life. Oral candidiasis is a common fungal infection observed in dental practice, particularly in immunocompromised patients. The aim of this study was to evaluate the presence of oral mucositis and oral candidiasis in patients who underwent HSCT and their correlation with the ...

  5. Effect of chromic γ-irradiation with small doses on candidiasis development in white rats

    Rats continuously exposed to 2 rads/day during eight months (cumulative dose of 400 rads) and nonirradiated rats were infected with a candida cells administered intravenously. All the irradiated animals died ten days after infection while only ten per cent of the control animals died for the same period of time. A morphological study has revealed candidiasis in the irradiated rats; changes, mainly in the kidneys, and formation of candidiasis granulomas have been detected in the control animals

  6. The value of prophylactic (monthly) clotrimazole versus empiric self-treatment in recurrent vaginal candidiasis.

    Fong, I W

    1994-01-01

    OBJECTIVE--To determine the comparative efficacy and cost benefit of prophylactic monthly (perimenstrual) clotrimazole, versus empiric self-treatment with the same agent at the onset of symptoms in recurrent vulvovaginal candidiasis. DESIGN--Prospective, randomised, open cross-over study of women with proven recurrent vulvovaginal candidiasis. Clinical and microbiological assessments were done every two months for 12 months. SETTING--Women's Clinic of a University Teaching Hospital. SUBJECTS-...

  7. Risk factors and outcome in patients with primary sclerosing cholangitis with persistent biliary candidiasis

    Rupp, Christian; Bode, Konrad Alexander; Chahoud, Fadi; Wannhoff, Andreas; Friedrich, Kilian; Weiss, Karl-Heinz; Sauer, Peter; Stremmel, Wolfgang; Gotthardt, Daniel Nils

    2014-01-01

    Background Candidiasis is commonly observed in patients with primary sclerosing cholangitis (PSC), but the clinical risk factors associated with its presence have not been fully investigated. In this study, we aimed to analyse the incidence, risk factors, and transplantation-free survival in primary sclerosing cholangitis (PSC) patients with persistent biliary candidiasis. Methods We retrospectively analysed patients diagnosed with PSC who were admitted to our department during 2002 to 2012. ...

  8. Optimal management of oropharyngeal and esophageal candidiasis in patients living with HIV infection

    Vazquez, Jose A.

    2010-01-01

    Jose A VazquezDivision of Infectious Diseases, Henry Ford Hospital, Wayne State University School of Medicine, Detroit, MI, USAAbstract: Mucocutaneous candidiasis is frequently one of the f irst signs of human immunodeficiency virus (HIV) infection. Over 90% of patients with AIDS will develop oropharyngeal candidiasis (OPC) at some time during their illness. Although numerous antifungal agents are available, azoles, both topical (clotrimazole) and systemic (fluconazole, itraconazole, voricona...

  9. Determinants of Incident Vulvovaginal Candidiasis in Human Immunodeficiency Virus-Positive Women

    Debra Matityahu; Emma Shifrin; Howard Minkoff; Joseph Feldman

    2000-01-01

    Objective: Mucosal infections including vulvovaginal candidiasis are a common problem for women with human immunodeficiency virus (HIV) infection. Our objective was to determine which factors predict the development of symptomatic disease among HIV-infected women.Materials and Methods: In a prospective study from 1991 to 1995, 205 HIV-positive women were evaluated every 6 months for occurrences of vulvovaginal candidiasis. Included in the study were all initially asymptomatic women, whether t...

  10. Recent mouse and rat methods for the study of experimental oral candidiasis

    Costa, Anna CBP; Pereira, Cristiane A; Junqueira, Juliana C; Jorge, Antonio OC

    2013-01-01

    The Candida genus expresses virulence factors that, when combined with immunosuppression and other risk factors, can cause different manifestations of oral candidiasis. The treatment of mucosal infections caused by Candida and the elucidation of the disease process have proven challenging. Therefore, the study of experimentally induced oral candidiasis in rats and mice is useful to clarify the etiopathology of this condition, improve diagnosis, and search for new therapeutic options because t...

  11. Protection against murine disseminated candidiasis mediated by a Candida albicans-specific T-cell line.

    Sieck, T G; Moors, M A; Buckley, H R; Blank, K J

    1993-01-01

    The role of T lymphocytes in disseminated candidiasis in a mouse model of irradiation-induced immunosuppression was investigated. A continuously cultured Candida albicans-specific T-cell line mediated protection of sublethally irradiated mice from disseminated candidiasis as measured by both the fungal load in the kidneys and mortality. These results are the first to demonstrate directly a role for antigen-specific T cells in the protective immune response against murine disseminated candidia...

  12. Risk factors and outcome in patients with primary sclerosing cholangitis with persistent biliary candidiasis

    Rupp, Christian; Bode, Konrad Alexander; Chahoud, Fadi; Wannhoff, Andreas; Friedrich, Kilian; Weiss, Karl-Heinz; Sauer, Peter; Stremmel, Wolfgang; Gotthardt, Daniel N

    2014-01-01

    Background: Candidiasis is commonly observed in patients with primary sclerosing cholangitis (PSC), but the clinical risk factors associated with its presence have not been fully investigated. In this study, we aimed to analyse the incidence, risk factors, and transplantation-free survival in primary sclerosing cholangitis (PSC) patients with persistent biliary candidiasis. Methods: We retrospectively analysed patients diagnosed with PSC who were admitted to our department during 2002 to 20...

  13. US and MR imaging of candidiasis of the nervous system in premature infants: two case reports

    Candidiasis of central nervous system (CNS) is rare condition and like other opportunistic fungal infections, most commonly occurs in immune-compromised patients. Because of the increasing use of antibiotics and the improving survival rate of premature infants requiring intensive care, the incidence of fungal infection in the brain has increased. We report the findings of ultrasonography and MR imaging in two cases of candidiasis of the CNS in premature infants

  14. US and MR imaging of candidiasis of the nervous system in premature infants: two case reports

    Kim, Kyo Nam; Woo, Joung Joo; Bahk, Yong Whee; Kim, Soon Yong; Kim, Eun Ryoung [Sungae Hospital, Seoul (Korea, Republic of)

    2001-07-01

    Candidiasis of central nervous system (CNS) is rare condition and like other opportunistic fungal infections, most commonly occurs in immune-compromised patients. Because of the increasing use of antibiotics and the improving survival rate of premature infants requiring intensive care, the incidence of fungal infection in the brain has increased. We report the findings of ultrasonography and MR imaging in two cases of candidiasis of the CNS in premature infants.

  15. A Clinician's Guide to the Diagnosis and Treatment of Candidiasis in Patients with Psoriasis.

    Armstrong, April W; Bukhalo, Michael; Blauvelt, Andrew

    2016-08-01

    Many of the molecular pathways associated with psoriasis pathogenesis are also involved in host defense mechanisms that protect against common pathogens. Candida can stimulate the production of cytokines that trigger or exacerbate psoriasis, and many systemic psoriasis treatments may put patients at increased risk for developing oral, cutaneous, and genitourinary candidiasis. Therefore, dermatologists should regularly screen patients with psoriasis for signs of Candida infection, and take steps to effectively treat these infections to prevent worsening of psoriasis symptoms. This review provides an overview of candidiasis epidemiology in patients with psoriasis, followed by a primer on the diagnosis and treatment of superficial Candida infections, with specific guidance for patients with psoriasis. Candidiasis in patients with psoriasis typically responds to topical or oral antifungal therapy. While biologic agents used to treat moderate-to-severe psoriasis, such as tumor necrosis factor-α inhibitors and interleukin-17 inhibitors, are known to increase patients' risk of developing localized candidiasis, the overall risk of infection is low, and candidiasis can be effectively managed in most patients while receiving systemic psoriasis therapies. Thus, the development of candidiasis does not usually necessitate changes to psoriasis treatment regimens. PMID:27435194

  16. Frequency of vaginal candidiasis in pregnant women attending routine antenatal clinic

    To determine the frequency of vaginal candidiasis in clinically symptomatic and asymptomatic cases of pregnant women attending routine antenatal clinic. A total of 110 pregnant women were nonrandomly recruited by convenient sampling. The studied variables included the demographic data information on parity, trimester of pregnancy, presence of vaginal discharge and the presence or absence of diabetes. Vulva and vagina were inspected for signs of inflammation and discharge with sterile speculum and vaginal specimens were collected with sterile cotton tipped swabs. Swabs were subjected to Gram staining and examined microscopically for the diagnosis of candidiasis. The frequency of vaginal candidiasis during pregnancy was found to be 38%, in which 27% were symptomatic and 11% were asymptomatic group. Increased ratio of infection was observed in multigravida and diabetic women. There was no marked differences in results with respect to age and trimester of pregnancy. Although there is generally a high frequency of vaginal candidiasis, an increased ratio of vaginal candidiasis in multigravida and diabetic pregnant women requires these women to be routinely screened for vaginal candidiasis regardless of symptomatic status. (author)

  17. FACTORS RELATED TO ORAL CANDIDIASIS IN ELDERLY USERS AND NON-USERS OF REMOVABLE DENTAL PROSTHESES

    Cyra Maria Pires de Carvalho BIANCHI

    2016-01-01

    Full Text Available This study examined the association between oral candidiasis in elderly users and nonusers of prosthesis and its predisposing factors. To this end, we performed a cross-sectional study where saliva samples from 48 patients were collected they used prosthesis and 43 patients (control group who did not use. Among the 91 patients, Candida spp were isolated in 40 (83.3% who used prosthesis and in 23 (53.5% in the control group. A statistically significant association was determined between the two groups, the isolation of yeasts and dental prosthesis (p < 0.05, OR = 4.3. The most common etiological agent was Candida albicans (37 isolates, with 23 (62.2% in the denture group and 14 (37.8% (control group. Among patients who presented clinical manifestations of oral candidiasis (n = 24, 83.3% (n = 20 belonged to the group that wore dentures, while only 16.7% (n = 4 belonged to the control group. Elderly patients with diabetes had 4.4 times higher estimated risk of developing oral candidiasis when compared with individuals without this condition. There was no statistically significant association between being user prostheses and have diabetes with the onset of candidiasis. No statistically significant association was determined between xerostomia, use of prosthesis and oral candidiasis. The use of prosthetics and poor oral hygiene in elderly patients predisposes to the development of oral candidiasis.

  18. The Recurrent Vulvovaginal Candidiasis: Proposal of a Personalized Therapeutic Protocol

    Murina, F.; Graziottin, A.; Felice, R.; Radici, G. L.; Di Francesco, S.

    2011-01-01

    Background. Recurrent vulvovaginal candidiasis (RVC) is an increasing challenge in clinical practice. Objective. The purpose of this study was to reduce the episodes of RVC through the intake of fluconazole 200 mg/dose with a personalized regimen at growing administration intervals with a probiotic. Method. 55 patients received a 200 mg fluconazole as an induction dose for 3 alternate days. Symptoms resolution after 10–14 days made the patients eligible to continue with a maintenance therapy of fluconazole weekly for one month, followed by 200 mg after 10, 15, 20 and 30 days. Patients were allowed to move on to the next level of maintenance therapy only if they were symptom free. Patients were also given a probiotic with Beta Glucan and Echinacea Purpurea. Results. Among the 55 patients enrolled, four (7%) have withdrawn after the induction phase. 51 patients completed the whole therapeutic maintenance period, and eight (15,6%) experienced a recurrence before the end of the therapy. Five women (9,8%) relapsed (two after 2 months and three after 6 months). Conclusion. The positive results of our study prove the effectiveness of an individualized protocol for a rather short period, with a slowly decreasing administration of fluconazole + probiotic. PMID:21845227

  19. Susceptibility to Vaginal Candidiasis under Different Conditions in Mice

    TAN Juan; LI Jiawen; CHEN Shanjuan; WU Yan; QIN Fang; DING Juan; CAO Fei; ZHANG Shaoru

    2005-01-01

    In order to study the susceptibility of murine vaginal mucosa to Candida albicans under different conditions, vaginal lavage fluid and vaginal tissue of mice were observed and compared be tween murine models with normal immune system (estrogen-treated mice) and immunosuppressed murine model, and between primary infection model of vaginal candidiasis and secondary infection one. The average level of colony forming unit (CFU) from the immuosuppressed group was higher than that from estrogen-treated group at each time point and the peak time was delayed. The differences between the two groups were statistically significant (P<0.05) from the fourth day after in oculation. A significant difference existed in the average level of CFU between the control group and the estrogen-treated group (P<0.05), and between the control group and the immuosuppressed group (P<0.01). It was concluded that the vaginal mucosa from the immunosuppressed mice is more susceptible to Candida albicans and no difference is found in susceptibility between mice with primary infection and secondary infection.

  20. Systemic vs. Topical Therapy for the Treatment of Vulvovaginal Candidiasis

    Sebastian Faro

    1994-01-01

    Full Text Available It is estimated that 75% of all women will experience at least 1 episode of vulvovaginal candidiasis (VVC during their lifetimes. Most patients with acute VVC can be treated with short-term regimens that optimize compliance. Since current topical and oral antifungals have shown comparably high efficacy rates, other issues should be considered in determining the most appropriate therapy. It is possible that the use of short-duration narrow-spectrum agents may increase selection of more resistant organisms which will result in an increase of recurrent VVC (RVVC. Women who are known or suspected to be pregnant and women of childbearing age who are not using a reliable means of contraception should receive topical therapy, as should those who are breast-feeding or receiving drugs that can interact with an oral azole and those who have previously experienced adverse effects during azole therapy. Because of the potential risks associated with systemic treatment, topical therapy with a broad-spectrum agent should be the method of choice for VVC, whereas systemic therapy should be reserved for either RVVC or cases where the benefits outweigh any possible adverse reactions.

  1. Chemokine receptor Ccr1 drives neutrophil-mediated kidney immunopathology and mortality in invasive candidiasis.

    Michail S Lionakis

    Full Text Available Invasive candidiasis is the 4(th leading cause of nosocomial bloodstream infection in the US with mortality that exceeds 40% despite administration of antifungal therapy; neutropenia is a major risk factor for poor outcome after invasive candidiasis. In a fatal mouse model of invasive candidiasis that mimics human bloodstream-derived invasive candidiasis, the most highly infected organ is the kidney and neutrophils are the major cellular mediators of host defense; however, factors regulating neutrophil recruitment have not been previously defined. Here we show that mice lacking chemokine receptor Ccr1, which is widely expressed on leukocytes, had selectively impaired accumulation of neutrophils in the kidney limited to the late phase of the time course of the model; surprisingly, this was associated with improved renal function and survival without affecting tissue fungal burden. Consistent with this, neutrophils from wild-type mice in blood and kidney switched from Ccr1(lo to Ccr1(high at late time-points post-infection, when Ccr1 ligands were produced at high levels in the kidney and were chemotactic for kidney neutrophils ex vivo. Further, when a 1∶1 mixture of Ccr1(+/+ and Ccr1(-/- donor neutrophils was adoptively transferred intravenously into Candida-infected Ccr1(+/+ recipient mice, neutrophil trafficking into the kidney was significantly skewed toward Ccr1(+/+ cells. Thus, neutrophil Ccr1 amplifies late renal immunopathology and increases mortality in invasive candidiasis by mediating excessive recruitment of neutrophils from the blood to the target organ.

  2. Clinical practice guidelines for the management of candidiasis: 2009 update by the Infectious Diseases Society of America.

    Pappas, P.G.; Kauffman, C.A.; Andes, D.; Benjamin Jr., D.K.; Calandra, T.; Edwards, J.E.; Filler, S.G.; Fisher, J.F.; Kullberg, B.J.; Ostrosky-Zeichner, L.; Reboli, A.C.; Rex, J.H.; Walsh, T.J.; Sobel, J.D.

    2009-01-01

    Guidelines for the management of patients with invasive candidiasis and mucosal candidiasis were prepared by an Expert Panel of the Infectious Diseases Society of America. These updated guidelines replace the previous guidelines published in the 15 January 2004 issue of Clinical Infectious Diseases

  3. Clinical heterogeneity of dominant chronic mucocutaneous candidiasis disease: presenting as treatment-resistant candidiasis and chronic lung disease.

    Dotta, Laura; Scomodon, Omar; Padoan, Rita; Timpano, Silviana; Plebani, Alessandro; Soresina, Annarosa; Lougaris, Vassilios; Concolino, Daniela; Nicoletti, Angela; Giardino, Giuliana; Licari, Amelia; Marseglia, Gianluigi; Pignata, Claudio; Tamassia, Nicola; Facchetti, Fabio; Vairo, Donatella; Badolato, Raffaele

    2016-03-01

    In gain-of-function STAT1 mutations, chronic mucocutaneous candidiasis disease (CMCD) represents the phenotypic manifestation of a complex immunodeficiency characterized by clinical and immunological heterogeneity. We aimed to study clinical manifestations, long-term complications, molecular basis, and immune profile of patients with dominant CMCD. We identified nine patients with heterozygous mutations in STAT1, including novel amino acid substitutions (L283M, L351F, L400V). High risk of azole-resistance was observed, particularly when intermittent regimens of antifungal treatment or use of suboptimal dosage occurs. We report a case of Cryptococcosis and various bacterial and viral infections. Risk of developing bronchiectasis in early childhood or gradually evolving to chronic lung disease in adolescent or adult ages emerges. Lymphopenia is variable, likely progressing by adulthood. We conclude that continuous antifungal prophylaxis associated to drug monitoring might prevent resistance to treatment; prompt diagnosis and therapy of lung disease might control long-term progression; careful monitoring of lymphopenia-related infections might improve prognosis. PMID:26732859

  4. Frequency and Etiology of Vulvovaginal Candidiasis in Women Referred to a Gynecological Center in Babol, lran

    Seddigheh Esmaeilzadeh

    2009-01-01

    Full Text Available Background: Vulvovaginal candidiasis is the most common fungal disease in sexually activewomen of which Candida albicans is the primary etiologic agent.Materials and Methods: This cross-sectional study was performed to determine the etiologic agentsof vulvovaginal candidiasis amongst 2000 women who presented to the Gynecological Center ofBabol Shahid Yahyanejad Educational Hospital from 2006-2007 with symptoms of vulvovaginalcandidiasis.Results: Discharge and pruritus were the most common symptoms in patients (80%. There were42 out of 97 (43.3% specimens which were positive for yeast. Of these, 80.95% belonged toCandida albicans and 14.29% were Candida krusei.Conclusion: Discharge and pruritus were the most common symptoms of vulvovaginal candidiasis.In our study, C. albicans was the most common species isolated from the specimens.

  5. Hepatosplenic and renal candidiasis in leukemic patients: CT spectrum before and after therapy

    Abdominal CT performed in 14 leukemic patients with systemic candidiasis and involvement of the liver, spleen, or kidneys revealed numerous low-density lesions in ten livers (71%), eight spleens (57%), and in the kidneys of three patients (21%). Biopsy of all livers and of three kidneys proved hepatic candidiasis in all (100%) and renal candidiasis in three patients (21%). After treatment with amphotericin B and splenectomy (one patient), CT disclosed abnormal livers in eleven (80%) patients, abnormal spleens in seven (53%), and abnormal kidneys in three patients (21%). Rebiopsy disclosed Candida infection in all livers and all abnormal kidneys, so the patients were treated with liposomal amphotericin B. Although the patients became asymptomatic, CT continued to show abnormal livers in five (35%) and abnormal spleens in two (16%) (the previously abnormal kidneys became normal). Rebiopsy of the abnormal livers showed focal fibrosis and necrosis. These findings emphasize the importance of clinical and pathologic correlation of CT appearance

  6. Chronic diarrhea due to duodenal candidiasis in a patient with a history of kidney transplantation.

    Nouri-Majalan, Nader; Moghaddasi, Sarasadat; Qane, Mohammad Davud; Shefaie, Farzane; Masoumi Dehshiri, Roghayyeh; Amirbaigy, Mohammad Kassem; Baghbanian, Mahmoud

    2014-11-01

    Candida infection in the small intestine is uncommon. We report an unusual case of duodenal candidiasis that presented as chronic diarrhea in a patient who had previously undergone kidney transplantation. A 60-year-old man presented with profuse watery diarrhea that had lasted 6 months 13 years after kidney transplantation. Upper gastrointestinal endoscopy results indicated candidiasis within the esophagus and duodenum. Biopsy results revealed active duodenitis with hyphal and yeast forms of Candida overlying the duodenal epithelium in periodic acid Schiff staining. The patient was successfully treated with fluconazole. After 6 months of follow-up, the patient had no complaint of diarrhea. Duodenal candidiasis may be the result of chronic diarrhea in patients with a history of kidney transplantation. PMID:25362226

  7. FACTORS RELATED TO ORAL CANDIDIASIS IN ELDERLY USERS AND NON-USERS OF REMOVABLE DENTAL PROSTHESES

    BIANCHI, Cyra Maria Pires de Carvalho; BIANCHI, Hélcio Aparecido; TADANO, Tomoko; de PAULA, Claudete Rodrigues; HOFFMANN-SANTOS, Hugo Dias; LEITE, Diniz Pereira; HAHN, Rosane Christine

    2016-01-01

    This study examined the association between oral candidiasis in elderly users and nonusers of prosthesis and its predisposing factors. To this end, we performed a cross-sectional study where saliva samples from 48 patients were collected they used prosthesis and 43 patients (control group) who did not use. Among the 91 patients, Candida spp were isolated in 40 (83.3%) who used prosthesis and in 23 (53.5%) in the control group. A statistically significant association was determined between the two groups, the isolation of yeasts and dental prosthesis (p xerostomia, use of prosthesis and oral candidiasis. The use of prosthetics and poor oral hygiene in elderly patients predisposes to the development of oral candidiasis. PMID:27007560

  8. Probiotics for prevention of recurrent vulvovaginal candidiasis: a review.

    Falagas, Matthew E; Betsi, Gregoria I; Athanasiou, Stavros

    2006-08-01

    Vulvovaginal candidiasis (VVC) is a common infection affecting the quality of life of many women. Probiotics have been investigated as possible agents for the prevention of recurrences of VVC. We reviewed the available literature. In some studies the development of VVC was associated with either a low number of lactobacilli in the vagina or with the presence of H2O2-non-producing vaginal lactobacilli, although there are a few studies not supporting these statements. In addition, in vitro studies have shown that lactobacilli can inhibit the growth of Candida albicans and/or its adherence on the vaginal epithelium. The results of some clinical trials support the effectiveness of lactobacilli, especially Lactobacillus acidophilus, Lactobacillus rhamnosus GR-1 and Lactobacillus fermentum RC-14, administered either orally or intravaginally in colonizing the vagina and/or preventing the colonization and infection of the vagina by C. albicans, while the results of a small number of clinical trials do not corroborate these findings. Nevertheless, most of the relevant clinical trials had methodological problems such as small sample size, no control group (placebo) and included women without confirmed recurrent VVC, and thus they are not reliable for drawing definitive conclusions. Thus, the available evidence for the use of probiotics for prevention of recurrent VVC is limited. However, the empirical use of probiotics may be considered in women with frequent recurrence of VVC (more than three episodes per year), especially for those who have adverse effects from or contraindications for the use of antifungal agents, since adverse effects of probiotics are very rare. In any case women should be clearly informed about the unproven usefulness of probiotics for this purpose. In conclusion, despite the promising results of some studies, further research is needed to prove the effectiveness of probiotics in preventing the recurrences of VVC and to allow their wide use for this

  9. Primary renal candidiasis: fungal mycetomas in the kidney

    Fungal infections of the urinary tract have a predilection for drainage structures rather than for the renal parenchyma. Of the causal factors, diabetes mellitus, immunosuppressed states, AIDS and prematurity are those most commonly encountered. The case of a young, diabetic man whose chief clinical presentation was dysuria is described. On further examination he was found to harbour fungal balls in the right kidney. Radiological manifestations of acute pyelonephritis were also present. Although primary renal candidiasis is often commensurate with systemic fungaemia, he displayed none of the clinical features of disseminate infection and, hence, was treated conservatively with oral antifungal agents. Fortuitously, spontaneous passage of fungal particulate matter in urine was later reported. A significant increase in the incidence of fungal cystitis has been found in recent years; however, the patient presents with many non-specific features of cystitis. Both sonography and CT show thickening of the bladder wall but, again, this lacks specificity. In the rare instance of prostate involvement, low attenuation foci on CT are seen within the gland. Despite the existence of a large number of fungal species, only a few are pathogenic to humans. Of those that cause disease in the urinary tract, Candida albicans is the most frequently encountered. A highly characteristic finding in such infections is of fungal balls, which are made up of aggregates of mycelia. However, care should be exercised in interpretation as a host of other conditions can mimic fungal bezoars. Although a CT scan at initial examination may qualify as the more descriptive, sonography provides a serial non-invasive means of evaluating the urinary tract. When in doubt, a urine culture clinches the diagnosis. Copyright (2002) Blackwell Science Pty Ltd

  10. Clinical and immunological data of nine patients with chronic mucocutaneous candidiasis disease.

    Dotta, Laura; Scomodon, Omar; Padoan, Rita; Timpano, Silviana; Plebani, Alessandro; Soresina, Annarosa; Lougaris, Vassilios; Concolino, Daniela; Nicoletti, Angela; Giardino, Giuliana; Licari, Amelia; Marseglia, Gianluigi; Pignata, Claudio; Tamassia, Nicola; Facchetti, Fabio; Vairo, Donatella; Badolato, Raffaele

    2016-06-01

    This paper describes the heterogeneous clinical phenotype of a cohort of nine patients diagnosed with heterozygous mutations in STAT1. We report data of extended immunophenotyping over time and we show lung damage in four patients. The increased phosphorylation of STAT1 in response to IFNγ and IFNα stimulation proves the gain-of-function nature of the defects. The data are supplemental to our original article concurrently published "Clinical heterogeneity of dominant chronic mucocutaneous candidiasis disease: presenting as treatment-resistant candidiasis and chronic lung disease" [1], where additional results and interpretation of our research can be found. PMID:26981552

  11. Oral candidiasis in Human Immunodeficiency Virus (HIV infected individuals in Iran

    Farzad Katiraee

    2010-04-01

    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Oropharyngeal candidiasis is the most frequent fungal infection in HIV patients. The aims of this study were to appraise prevalence of oropharyngeal candidiasis and to determine factors associated with oropharyngeal candidiasis and oral yeast colonization among Iranian HIV patients."n"nMethods: The patients were composed 150 Iranian HIV positive individuals referred to Iranian Research Center for HIV & Aids (IRCHA, Imam Khomeini Hospital complex in Tehran, Iran. Oral samples were obtained and cultured on mycological media. TCD4 lymphocyte count/percentage was measured and patients were categorized. Patients evaluated for some risk factors for oropharyngeal candidiasis and oral candida colonization."n"nResults: Fifty nine percent of patients were presented with oropharyngeal candidiasis and the carriage rate of yeasts was 116 (77.2%. The most frequent isolated candida species were Candida albicans (102 50.2% and Candida glabrata (45 22%. Thrush in 57(38%, perleche in 30(20% and erythematous lesions in 7(4.7% of patients were observed. Significant differences in TCD4 count (p=0.01, gender (p=0.02, antifungal therapy (p=0.001, smoking (p=0.02, and intravenous drug use (p=0.03, between asymptomatic and

  12. Incidence and Clinical Predictors of Ocular Candidiasis in Patients with Candida Fungemia

    Ayesha Khalid

    2014-01-01

    Full Text Available Purpose. The aim of this study is to determine the incidence and the predictors of ocular candidiasis among patient with Candida fungemia. Methods. We retrospectively reviewed the charts of all patients diagnosed with candidemia at the University of Kansas Medical Center during February 2000–March 2010. Data regarding patients’ demographics, clinical characteristics, laboratory results, and ophthalmology examination findings were collected. Results. A total of 283 patients with candidemia were enrolled. The mean age (± standard deviation was 55 ± 18 years; 66% were male. The most commonly isolated Candida species were C. albicans (54%, C. parapsilosis (20%, C. glabrata (13%, and C. tropicalis (8%. Only 144 (51% patients were evaluated by ophthalmology; however, the proportion of patients who were formally evaluated by an ophthalmologist increased during the study period (9%in 2000 up to 73%in 2010; P<0.0001. Evidence of ocular candidiasis was present in 18 (12.5% patients. Visual symptoms were reported by 5 of 18 (28% patients. In multivariable analysis, no predictors of ocular candidiasis were identified. Conclusions. The incidence of ocular candidiasis among patients with fungemia remains elevated. Most patients are asymptomatic and therefore all patients with candidemia should undergo fundoscopic examination to rule out ocular involvement.

  13. Evaluation of Prevalence of Oral Candidiasis in Patients Using Complete Denture Wears

    P Tavakkol

    2001-06-01

    Full Text Available Denture stomatotos os a common problem of the denture wears, and its etiology is not clear from the literatures. Some studies show that the aetiology is candida albicans, while other reports point out the other agents including candida albicans. The present study was designed to evaluate the prevalence of oral candidiasis in patients using complete denture with different datas. 50 subjects all wearing complete denture participated in this study. The palatal mucosa was swabbed and swabs were cultured in Sabourauds medium containing 0.005% chloramphenicol then cultures were tested with common mycological lab tests. In 80% of patients oral candidiasis have been diagnosed. In the investigation a direct relation between the oral candidiasis and removal of denture at night, denture cleanliness, presence of any suction and symptoms of denture stomatitis was shown. No relation between the oral candidiasis and sex, smoking habit, succeptible disease, angular cheilitis; the recent condition of denture and the retention of denture were found. The findings of our investigation indicates that candida albicans is the causative agent of denture stomatitis while other factors such as denture cleanliness, removal of denture at night and presence of any suction may be considered as well.

  14. Dynamical evaluation of vaginal micro-ecosystem in a Chinese woman with recurrent vulvovaginal candidiasis.

    Hu, Z Q; Zhou, W; Yue, X A; Mu, L Y; Jiang, Y M

    2015-01-01

    We reported a rare case of recurrent vulvovaginal candidiasis (RVVC) in this study. Through dynamic evaluation of the vaginal micro-ecosystem, we found that only depuratory degree, spores, blastospores, and hyphae were specific indicators and the "barometer" of RVVC development. Therefore, an understanding of vaginal micro-ecological changes can help clinicians to improve the treatment of patients with RVVC. PMID:25867410

  15. Contribution of Candida albicans ALS1 to the Pathogenesis of Experimental Oropharyngeal Candidiasis

    Kamai, Yasuki; Kubota, Mikie; Kamai, Yoko; Hosokawa, Tsunemichi; Fukuoka, Takashi; Filler, Scott G.

    2002-01-01

    We investigated the contribution of Candida albicans ALS1, which encodes a candidal adhesin, to the pathogenesis of experimental murine oropharyngeal candidiasis. Our results indicate that the ALS1 gene product is important for the adherence of the organism to the oral mucosa during the early stage of the infection.

  16. Hepatic scar in a case of healed candidiasis showing prolonged enhancement on CT

    Itai, Yuji; Yashiro, Naobumi

    1987-08-01

    A patient with acute myelocytic leukemia recovering from hepatic candidiasis after long-term administration of amphotericin B had large scar in the liver which showed prominent prolonged enhancement on postcontrast CT. Prolonged enhancement can occur in regions other than hepatic masses.

  17. New US and CT patterns of hepatic and spienic candidiasis in immunocompromised patients

    Nine immunocompromised cancer patients with tissue-proved candidiasis underwent a total of eight serial abdominal US studies and 53 CT examinations. Four US patterns of hepatic and splenic candidiasis were recognized. Type 1 was a ''wheel within a wheel'', or a central hypoechoic nidus surrounded by a dense band, in turn surronded by a rim of hypoechoic tissue. Type 2 was the classic ''bull's eye'' lesion. Type 3, the most common, was a uniformly hypoechoic lesion, 1-4 cm in diameter. Type 4 was a small, dense, echogenic focus with no surrounding lucency and varying amounts of posterior shadowing. Types 1,2 and 3 were seen early in infection; types 3 and 4 were present later. Lesion types 2,3 and 4 were also identified on CT scans. In addition, periportal linear areas of increased attenuation, possibly calcified, were identified on follow-up, non-contrast-enhanced CT. Some abscesses were better seen on non-contrast-enhanced CT scans, while others became visible only after iodinated contrast medium was administered intravenously. Although lesions not seen on US were often seen on CT, the opposite was also true. In two cases pathologic proof of candidiasis was established even when all imaging studies were negative. Patients should be studied by US and by CT before and after contrast agent administration for maximum sensitivity. Even when both US and CT are negative, if there is a strong clinical suspicion of candidiasis, open biopsy is recommended

  18. Diagnosis and management of invasive candidiasis in the ICU: an updated approach to an old enemy.

    Calandra, Thierry; Roberts, Jason A; Antonelli, Massimo; Bassetti, Matteo; Vincent, Jean-Louis

    2016-01-01

    Invasive fungal infections, particularly those caused by Candida species, are not uncommon in critically ill patients and are associated with considerable morbidity and mortality. Diagnosis and management of these infections can be challenging. In this review, we will briefly discuss recent epidemiological data on invasive candidiasis and current diagnostic approaches before concentrating on antifungal treatments. PMID:27230564

  19. The Role of Dectin-2 for Host Defense Against Disseminated Candidiasis.

    Ifrim, Daniela C; Quintin, Jessica; Courjol, Flavie; Verschueren, Ineke; van Krieken, J Han; Koentgen, Frank; Fradin, Chantal; Gow, Neil A R; Joosten, Leo A B; van der Meer, Jos W M; van de Veerdonk, Frank; Netea, Mihai G

    2016-04-01

    Despite the fact that Candida albicans is an important human fungal pathogen and Dectin-2 is a major pattern recognition receptor for fungi, our knowledge regarding the role of Dectin-2 for the host defense against disseminated candidiasis is limited. Dectin-2 deficient (Dectin-2(-/-)) mice were more susceptible to systemic candidiasis, and the susceptibility was mirrored by an elevated fungal load in the kidneys that correlated with the presence of large inflammatory foci. Phagocytosis of Candida by the macrophages lacking the Dectin-2 receptor was moderately decreased, while production of most of the macrophage-derived cytokines from Dectin-2(-/-) mice with systemic candidiasis was decreased. No striking differences among several Candida mutants defective in mannans could be detected between naïve wild-type and Dectin-2(-/-) mice, apart from the β-mannan-deficient bmt1Δ/bmt2Δ/bmt5Δ triple mutant, suggesting that β-mannan may partially mask α-mannan detection, which is the major fungal structure recognized by Dectin-2. Deciphering the mechanisms responsible for host defense against the majority of C. albicans strains represents an important step in understanding the pathophysiology of systemic candidiasis, which might lead to the development of novel immunotherapeutic strategies. PMID:27046240

  20. Hepatic scar in a case of healed candidiasis showing prolonged enhancement on CT

    A patient with acute myelocytic leukemia recovering from hepatic candidiasis after long-term administration of amphotericin B had large scar in the liver which showed prominent prolonged enhancement on postcontrast CT. Prolonged enhancement can occur in regions other than hepatic masses. (author)

  1. Abdominal aortic aneurysm in a premature neonate with disseminated candidiasis: Ultrasound and angiography

    Khoss, A.E.; Ponhold, W.; Pollak, A.; Schlemmer, M.; Weninger, M.

    1985-09-01

    When using ultrasound for detection of kidney enlargement, we found an acute abdominal aortic aneurysm secondary to aortitis arising from umbilical artery catheterisation in a premature neonate with systemic candidiasis. Aortography was performed to provide vascular details such as involvement of celiac, renal, iliac and femoral arteries.

  2. Gene polymorphisms in pattern recognition receptors and susceptibility to idiopathic recurrent vulvovaginal candidiasis

    Rosentul, D.C.; Delsing, C.E.; Jaeger, M.; Plantinga, T.S.; Oosting, M.; Costantini, I.; Venselaar, H.; Joosten, L.A.B.; Meer, J.W.M. van der; Dupont, B.; Kullberg, B.J.; Sobel, J.D.; Netea, M.G.

    2014-01-01

    OBJECTIVE: Approximately 5% of women suffer from recurrent vulvovaginal candidiasis (RVVC). It has been hypothesized that genetic factors play an important role in the susceptibility to RVVC. The aim of this study was to assess the effect of genetic variants of genes encoding for pattern recognition

  3. Abdominal aortic aneurysm in a premature neonate with disseminated candidiasis: Ultrasound and angiography

    When using ultrasound for detection of kidney enlargement, we found an acute abdominal aortic aneurysm secondary to aortitis arising from umbilical artery catheterisation in a premature neonate with systemic candidiasis. Aortography was performed to provide vascular details such as involvement of celiac, renal, iliac and femoral arteries. (orig.)

  4. Candidiasis esofágica en pacientes con sida. Estudio clínico y microbiológico

    Zenia Flores de Apodaca Verdura; Gerardo Martínez Machín; Aroldo Ruiz Pérez; Carlos M Fernández Andreu; Mayra Muné Jiménez; Mayda Perurena Lancha

    1998-01-01

    Se estudiaron 18 pacientes con sida, que presentaban síntomas gastrointestinales altos o lesiones en la cavidad oral sugestivos de candidiasis. Se obtuvieron datos clínicos, muestras de la cavidad oral, biopsias y cepillado esofágico, así como suero de todos los pacientes. El síntoma que principalmente se observó en la candidiasis esofágica fue la disfagia; la candidiasis oral se comportó de forma asintomática. Candida albicans fue la especie que más se aisló con predominio del serotipo A. Se...

  5. Microbiological Screening of Irish Patients with Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy Reveals Persistence of Candida albicans Strains, Gradual Reduction in Susceptibility to Azoles, and Incidences of Clinical Signs of Oral Candidiasis without Culture Evidence▿†

    McManus, Brenda A.; McGovern, Eleanor; Moran, Gary P.; Healy, Claire M.; Nunn, June; Fleming, Pádraig; Costigan, Colm; Derek J. Sullivan; Coleman, David C.

    2011-01-01

    Patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) are prone to chronic mucocutaneous candidiasis, which is often treated with azoles. The purpose of this study was to characterize the oral Candida populations from 16 Irish APECED patients, who comprise approximately half the total number identified in Ireland, and to examine the effect of intermittent antifungal therapy on the azole susceptibility patterns of Candida isolates. Patients attended between one ...

  6. Immunohistochemical study of in vivo and in vitro IgA coating of candida species in vulvovaginal candidiasis.

    Böhler, K; Klade, H; Poitschek, C; Reinthaller, A.

    1994-01-01

    OBJECTIVE--To evaluate whether quantitative or qualitative IgA deficiencies in cervicovaginal secretions can be identified in patients with recurrent vulvovaginal candidiasis. DESIGN--Prospective and controlled study. SETTING--Department of Dermatology, University of Vienna. SUBJECTS--30 patients with symptomatic and recurrent vulvovaginal candidiasis at the time of their presentation. 30 healthy women as a control group. INTERVENTION--Blood samples were drawn for measurement of serum IgA lev...

  7. Antibody blockade of IL-17 family cytokines in immunity to acute murine oral mucosal candidiasis.

    Whibley, Natasha; Tritto, Elaine; Traggiai, Elisabetta; Kolbinger, Frank; Moulin, Pierre; Brees, Dominique; Coleman, Bianca M; Mamo, Anna J; Garg, Abhishek V; Jaycox, Jillian R; Siebenlist, Ulrich; Kammüller, Michael; Gaffen, Sarah L

    2016-06-01

    Antibodies targeting IL-17A or its receptor, IL-17RA, are approved to treat psoriasis and are being evaluated for other autoimmune conditions. Conversely, IL-17 signaling is critical for immunity to opportunistic mucosal infections caused by the commensal fungus Candida albicans, as mice and humans lacking the IL-17R experience chronic mucosal candidiasis. IL-17A, IL-17F, and IL-17AF bind the IL-17RA-IL-17RC heterodimeric complex and deliver qualitatively similar signals through the adaptor Act1. Here, we used a mouse model of acute oropharyngeal candidiasis to assess the impact of blocking IL-17 family cytokines compared with specific IL-17 cytokine gene knockout mice. Anti-IL-17A antibodies, which neutralize IL-17A and IL-17AF, caused elevated oral fungal loads, whereas anti-IL-17AF and anti-IL-17F antibodies did not. Notably, there was a cooperative effect of blocking IL-17A, IL-17AF, and IL-17F together. Termination of anti-IL-17A treatment was associated with rapid C. albicans clearance. IL-17F-deficient mice were fully resistant to oropharyngeal candidiasis, consistent with antibody blockade. However, IL-17A-deficient mice had lower fungal burdens than anti-IL-17A-treated mice. Act1-deficient mice were much more susceptible to oropharyngeal candidiasis than anti-IL-17A antibody-treated mice, yet anti-IL-17A and anti-IL-17RA treatment caused equivalent susceptibilities. Based on microarray analyses of the oral mucosa during infection, only a limited number of genes were associated with oropharyngeal candidiasis susceptibility. In sum, we conclude that IL-17A is the main cytokine mediator of immunity in murine oropharyngeal candidiasis, but a cooperative relationship among IL-17A, IL-17AF, and IL-17F exists in vivo. Susceptibility displays the following hierarchy: IL-17RA- or Act1-deficiency > anti-IL-17A + anti-IL-17F antibodies > anti-IL-17A or anti-IL-17RA antibodies > IL-17A deficiency. PMID:26729813

  8. Factors involved in patient choice of oral or vaginal treatment for vulvovaginal candidiasis

    Sobel JD

    2013-12-01

    Full Text Available Jack D SobelDivision of Infectious Diseases, Wayne State University School of Medicine, Detroit, MI, USAAbstract: Vulvovaginal candidiasis (VVC is an extremely common cause of vaginal symptoms in women. Multiple antifungal products are available by either the oral or vaginal route, although no new drugs have become available for two decades. Given the therapeutic equivalence of the antimycotic agents and their routes of administration, the specific drug and formulation selected is entirely arbitrary in relation to final treatment outcome. Nevertheless, multiple factors affecting preference, both practitioner-dependent and patient-dependent, impact on selection of a specific drug and route of administration.Keywords: antifungal drugs, antimycotics, Candida vaginitis, vulvovaginal candidiasis

  9. Expression of Candida Albicans Secreted Aspartyl Proteinase in Acute Vaginal Candidiasis

    LIN Nengxing; FENG Jing; TU Yating; FENG Aiping

    2007-01-01

    In order to analyze the in vivo expression of Candida albicans secreted aspartyl proteinases (SAP) in human vaginal infection, the vaginal secretion from 29 human subjects was collected by vaginal swab, and the expression of SAP1-SAP6 was detected by reverse-transcriptase polymerase chain reaction using specific primer sets. It was found that Sap2 and Sap5 were the most common genes expressed during infection; Sap3 and Sap4 were detected in all subjects and all 6 SAP genes were simultaneously expressed in some patients with vaginal candidiasis. It was suggested that the SAP family is expressed by Candida albicans during infection in human and that Candida albicans infection is associated with the differential expression of individual SAP genes which may be involved in the pathogenesis of vaginal candidiasis.

  10. Invasive candidiasis in non neutropenic critically ill - need for region-specific management guidelines

    Armin Ahmed

    2015-01-01

    Full Text Available Use of antifungal agents has increased over past few decades. A number of risk factors such as immunosuppression, broad spectrum antibiotics, dialysis, pancreatitis, surgery, etc., have been linked with the increased risk of invasive candidiasis. Though there are various guidelines available for the use of antifungal therapy, local/regional epidemiology plays an important role in determining the appropriate choice of agent in situations where the offending organism is not known (i.e. empirical, prophylactic or preemptive therapy. Developing countries like India need to generate their own epidemiological data to facilitate appropriate use of antifungal therapy. In this article, the authors have highlighted the need for region-specific policies/guidelines for treatment of invasive candidiasis. Currently available Indian literature on candidemia epidemiology has also been summarized here.

  11. Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis.

    Matthews, R.; Burnie, J

    1989-01-01

    DNA fingerprinting was assessed as an improved typing system for Candida albicans aimed at speeding the implementation of cross infection control measures in outbreaks of systemic candidiasis. The study was carried out with 45 previously characterised isolates from five different outbreaks and with 96 unrelated isolates from a mixed control population. Sixteen different genotypes were produced. Results were obtainable within days, reproducibility was high, and there was good discrimination am...

  12. Endogenous thrombospondin-1 regulates leukocyte recruitment and activation and accelerates death from systemic candidiasis.

    Gema Martin-Manso

    Full Text Available Disseminated Candida albicans infection results in high morbidity and mortality despite treatment with existing antifungal drugs. Recent studies suggest that modulating the host immune response can improve survival, but specific host targets for accomplishing this goal remain to be identified. The extracellular matrix protein thrombospondin-1 is released at sites of tissue injury and modulates several immune functions, but its role in C. albicans pathogenesis has not been investigated. Here, we show that mice lacking thrombospondin-1 have an advantage in surviving disseminated candidiasis and more efficiently clear the initial colonization from kidneys despite exhibiting fewer infiltrating leukocytes. By examining local and systemic cytokine responses to C. albicans and other standard inflammatory stimuli, we identify a crucial function of phagocytes in this enhanced resistance. Subcutaneous air pouch and systemic candidiasis models demonstrated that endogenous thrombospondin-1 enhances the early innate immune response against C. albicans and promotes activation of inflammatory macrophages (inducible nitric oxide synthase⁺, IL-6(high, TNF-α(high, IL-10(low, release of the chemokines MIP-2, JE, MIP-1α, and RANTES, and CXCR2-driven polymorphonuclear leukocytes recruitment. However, thrombospondin-1 inhibited the phagocytic capacity of inflammatory leukocytes in vivo and in vitro, resulting in increased fungal burden in the kidney and increased mortality in wild type mice. Thus, thrombospondin-1 enhances the pathogenesis of disseminated candidiasis by creating an imbalance in the host immune response that ultimately leads to reduced phagocytic function, impaired fungal clearance, and increased mortality. Conversely, inhibitors of thrombospondin-1 may be useful drugs to improve patient recovery from disseminated candidiasis.

  13. Candidiasis in Pediatrics; Identification and In vitro Antifungal Susceptibility of the Clinical Isolates

    MOHAMMADI, R.; Ataei, B

    2016-01-01

    Background Candida species are normal microflora of oral cavity, vagina, and gastrointestinal tract. They are the third most prevalent cause of pediatric health care–associated bloodstream fungal infection. This study aimed to provide an epidemiological feature of candidiasis and also presents an antifungal susceptibility profile of clinical Candida isolates among children. Materials and Methods During July 2013 to February 2015, 105 patients from different hospitals of Isfahan, Iran, were ex...

  14. Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis

    Song, You Bum; Suh, Moo Kyu; Ha, Gyoung Yim; Kim, Heesoo

    2015-01-01

    Background The necessity of performing antifungal susceptibility tests is recently increasing because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMerieux, Marcy l'Etoile, France) is a rapid and easy-to-perform in vitro antifungal susceptibility test. Objective The purpose of this study was to determine the minimal inhibitory concentrations (MICs) of antifungal agents by using the Etest for Candida species isolated from patients with oral...

  15. Clinical and microbiological characteristics of symptomatic vulvovaginal candidiasis in HIV-seropositive women.

    Spinillo, A; G. Michelone; Cavanna, C; Colonna, L; Capuzzo, E.; De Nicola, S.

    1994-01-01

    OBJECTIVES--To evaluate the clinical and microbiological characteristics of symptomatic vaginal candidiasis in Human Immunodeficiency Virus (HIV)-seropositive women attending a gynaecologic outpatient clinic for sexually transmitted diseases (STDs). DESIGN--Vaginal, rectal and oral specimens from cases and controls were cultured for Candida spp. SUBJECTS--Eighty-four consecutive HIV-seropositive and 384 HIV-seronegative women with clinical signs of vulvovaginitis. SETTING--A gynaecological ou...

  16. Prospects for Development of a Vaccine to Prevent and Control Vaginal Candidiasis

    Fidel, Paul L.; Cutler, Jim E.

    2011-01-01

    A vaccine against recurrent vulvovaginal candidiasis (RVVC) would benefit a large number of women who suffer from this debilitating syndrome. To date, several antigen formulations have been tested with modest results. In this article, we review the latest vaccine study reported in the literature. The candidate is a β-glucan conjugate administered with a human compatible adjuvant. Results in a mouse model of vaginitis were again modest for protection. However, the study included live animal im...

  17. Patient preferences and treatment safety for uncomplicated vulvovaginal candidiasis in primary health care

    Hernández Susana; Iglesias Piñeiro M; Sanz Yolanda; Yepes Jeannet; Uriarte Beatriz; Vello Cuadrado Rocío A; González Paulino; Rojas Giraldo María J; Horcajuelo Jesús; del Pozo Marta; Teira Blanca; Rodríguez-Moñino Ana; Díaz Yolanda; De-Alba Romero Cristina; Wiesmann Elisa

    2011-01-01

    Abstract Background Vaginitis is a common complaint in primary care. In uncomplicated candidal vaginitis, there are no differences in effectiveness between oral or vaginal treatment. Some studies describe that the preferred treatment is the oral one, but a Cochrane's review points out inconsistencies associated with the report of the preferred way that limit the use of such data. Risk factors associated with recurrent vulvovaginal candidiasis still remain controversial. Methods/Design This wo...

  18. Appropriateness and cost-effectiveness in the treatment of invasive candidiasis in Internal Medicine Wards

    Enrico Tagliaferri; Francesco Menichetti

    2014-01-01

    Invasive candidiasis (IC), including candidemia, is a major cause of morbidity and mortality among patients and the majority of cases of candidemia are documented in Medical Wards. Early identification of patients at risk, knowledge of local epidemiology and prompt efforts to define etiologic diagnosis are pivotal to ensure appropriateness. Start with an echinocandin and switch to fluconazole when possible, seems to represent a useful strategy for the management of IC. The choice between the ...

  19. Transcriptomic Analysis of Vulvovaginal Candidiasis Identifies a Role for the NLRP3 Inflammasome

    Bruno, Vincent M.; Shetty, Amol C.; Yano, Junko; Fidel, Paul L.; Noverr, Mairi C.; Peters, Brian M.

    2015-01-01

    ABSTRACT Treatment of vulvovaginal candidiasis (VVC), caused most frequently by Candida albicans, represents a significant unmet clinical need. C. albicans, as both a commensal and a pathogenic organism, has a complex and poorly understood interaction with the vaginal environment. Understanding the complex nature of this relationship is necessary for the development of desperately needed therapies to treat symptomatic infection. Using transcriptome sequencing (RNA-seq), we characterized the e...

  20. Renal candidiasis in newborn: the ultrasonography as diagnostic and therapeutic method

    We present a case of systemic candidiasis in a low-weight newborn. Renal involvement presented as unilateral hydronephrosis secondary to the fungal construction. Ultrasound served not only as a diagnostic tool, but as a guide for the performance of performance of percutaneous nephrostomy which was included in the therapeutic approach to decompress the excretory pathway and allow local installation of anti fungal agents. (Author) 16 refs

  1. Mucocutaneous candidiasis: the IL-17 pathway and implications for targeted immunotherapy

    Huppler, Anna R; Bishu, Shrinivas; Sarah L Gaffen

    2012-01-01

    IL-17 and related cytokines are direct and indirect targets of selective immunosuppressive agents for the treatment of autoimmune diseases and other diseases of pathologic inflammation. Insights into the potential adverse effects of IL-17 blockade can be drawn from the experience of patients with deficiencies in the IL-17 pathway. A unifying theme of susceptibility to mucocutaneous candidiasis is seen in both mice and humans with a variety of genetic defects that converge on this pathway. Muc...

  2. Clinical assessment of oral mucositis and candidiasis compare to chemotherapic nadir in transplanted patients

    PATUSSI Cleverson

    2014-09-01

    Full Text Available Oral mucositis is a chief complication in patients undergoing hematopoietic stem cell transplantation (HSCT. It is considered a toxic inflammatory reaction that interferes with the patient’s recuperation and quality of life. Oral candidiasis is a common fungal infection observed in dental practice, particularly in immunocompromised patients. The aim of this study was to evaluate the presence of oral mucositis and oral candidiasis in patients who underwent HSCT and their correlation with the chemotherapeutic nadir (lowest possible outcome. We evaluated patients with different diagnoses who underwent HSCT at the Hospital Erasto Gaertner. No chemotherapeutic nadir curves could be associated with mucositis, and patients had different presentations of mucositis. No patient developed oral candidiasis during hospitalization. Together with cell counts, we collected demographic data including age, oral hygiene, habits harmful to health, and the use of oral prostheses. It was observed that patients who smoked cigarettes before hospitalization showed less mucositis, resulting in no feeding problems or other comorbid conditions due to the effect of mucositis. However, the nadir of the chemotherapy curve, in isolation, is not a predictive tool for the appearance (or no appearance of oral mucositis.

  3. Relationship between Host Survival and the Type of Immune Response in Different Organs during Disseminated Candidiasis

    2005-01-01

    To examine the relationship between host survival and the type of immune response in different organs during disseminated candidiasis, the murine model of disseminated candidiasis was established by injection with Candida albicans via tail vein. The survival time was observed for up to 60 days. And the expression levels of cytokines in the spleen and kidney, including IFN-γ and IL-4, were determined with RT-PCR. Our results showed that in the spleen, both non-fatal and fatal inoculum caused a type Ⅱ immune response with steady expression levels of IFN-γ and the obviously increased levels of IL-4. While in the kidney, non-fatal inoculum induced a type Ⅰ immune response with the obviously increased levels of IFN-γ and the steady expression levels of IL-4. However, fatal inoculum induced a type Ⅱ immune response with a constant expression of IFN-γ and the evidently increased levels of IL-4. It is concluded that in disseminated candidiasis, host survival is associated with the type of immune responses in the kidney, but not in the spleen.

  4. Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing

    Imabayashi, Yumi; Moriyama, Masafumi; Takeshita, Toru; Ieda, Shinsuke; Hayashida, Jun-Nosuke; Tanaka, Akihiko; Maehara, Takashi; Furukawa, Sachiko; Ohta, Miho; Kubota, Keigo; Yamauchi, Masaki; Ishiguro, Noriko; Yamashita, Yoshihisa; Nakamura, Seiji

    2016-01-01

    Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by Candida albicans. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other Candida or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-C. albicans, especially C. dubliniensis, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections. PMID:27305838

  5. Advanced topical drug delivery system for the management of vaginal candidiasis.

    Johal, Himmat Singh; Garg, Tarun; Rath, Goutam; Goyal, Amit Kumar

    2016-01-01

    Vaginal candidiasis or vulvovaginal candidiasis (VC) is a common mucosal infection of vagina, mainly caused by Candida species. The major symptoms of VC are dyspareunia, pruritis, itching, soreness, vagina as well as vulvar erythema and edema. Most common risk factors that lead to the imbalance in the vaginal micro biota are the use of antibiotics, pregnancy, diabetes mellitus, immuno suppression as in AIDS or HIV patients, frequent sexual intercourse, spermicide and intra-uterine devices and vaginal douching. Various anti-fungal drugs are available for effective treatment of VC. Different conventional vaginal formulations (creams, gels, suppositories, powder, ointment, etc.) for VC are available today but have limited efficacy because of lesser residence time on vaginal epithelium due to self-cleansing action of vagina. So to overcome this problem, an extended and intimate contact with vaginal mucosa is desired; which can be accomplished by utilizing mucoadhesive polymers. Mucoadhesive polymers have an excellent binding capacity to mucosal tissues for considerable period of time. This unique property of these polymers significantly enhances retention time of different formulations on mucosal tissues. Currently, various novel formulations such as liposomes, nano- and microparticles, micro-emulsions, bio-adhesive gel and tablets are used to control and treat VC. In this review, we focused on current status of vaginal candidiasis, conventional and nanotechnology inspired formulation approaches. PMID:24959937

  6. Efficacy and safety of miconazole for oral candidiasis: a systematic review and meta-analysis.

    Zhang, L-W; Fu, J-Y; Hua, H; Yan, Z-M

    2016-04-01

    The objective of this study is to assess the efficacy and safety of miconazole for treating oral candidiasis. Twelve electronic databases were searched for randomized controlled trials evaluating treatments for oral candidiasis and complemented by hand searching. The clinical and mycological outcomes, as well as adverse effects, were set as the primary outcome criteria. Seventeen trials were included in this review. Most studies were considered to have a high or moderate level of bias. Miconazole was more effective than nystatin for thrush. For HIV-infected patients, there was no significant difference in the efficacy between miconazole and other antifungals. For denture wearers, microwave therapy was significantly better than miconazole. No significant difference was found in the safety evaluation between miconazole and other treatments. The relapse rate of miconazole oral gel may be lower than that of other formulations. This systematic review and meta-analysis indicated that miconazole may be an optional choice for thrush. Microwave therapy could be an effective adjunct treatment for denture stomatitis. Miconazole oral gel may be more effective than other formulations with regard to long-term results. However, future studies that are adequately powered, large-scale, and well-designed are needed to provide higher-quality evidence for the management of oral candidiasis. PMID:26456226

  7. Treatment of Oral Candidiasis Using Photodithazine®- Mediated Photodynamic Therapy In Vivo

    G. Basso, Fernanda; de Souza Costa, Carlos Alberto; Bagnato, Vanderlei Salvador; Mima, Ewerton Garcia de Oliveira; Pavarina, Ana Cláudia

    2016-01-01

    This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine® (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (pcandidiasis. PMID:27253525

  8. Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing.

    Imabayashi, Yumi; Moriyama, Masafumi; Takeshita, Toru; Ieda, Shinsuke; Hayashida, Jun-Nosuke; Tanaka, Akihiko; Maehara, Takashi; Furukawa, Sachiko; Ohta, Miho; Kubota, Keigo; Yamauchi, Masaki; Ishiguro, Noriko; Yamashita, Yoshihisa; Nakamura, Seiji

    2016-01-01

    Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by Candida albicans. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other Candida or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-C. albicans, especially C. dubliniensis, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections. PMID:27305838

  9. Lipase Gene Expression of Resistant and Sensitive Candida Albicans to Fluconazole Isolated from Patients Suffering from Oral Candidiasis and Vaginal Candidiasis

    NasrollahiOmran, A. (PhD

    2015-01-01

    Full Text Available Background and Objective: With the development of drug resistance in strains of fungi, there is a considerable resistance of Candida albicans strains to fluconazole. Molecular studies are developing to determine the relationship of such a drug resistance with the increased gene expression of enzymes produced in drug-resistant Candida isolates. We aimed to evaluate the relationship between extracellular lipase gene (LIP8 expression of Candida albicans isolated from candidiasis and sensitivity or resistance to fluconazole. Material and Methods: Drug susceptibility of Candida albicans was performed in oral and vaginal candidiasis to determine the proportion of strains sensitive or resistant to fluconazole using NCCLS method. To evaluate and compare the expression of these genes in the susceptible and resistant strains, RT real-time PCR reaction was used. Results: Of 46 Candida albicans, 20 were susceptible, 12 were semi-susceptible and 14 were resistant to fluconazole. By using PCR reaction, the results showed that the expression of this gene in fluconazole-susceptible isolates was moderate, while it was high in the isolates resistant to fluconazole. Conclusion: The results of lipase gene (LIP8 expression showed that the additional expression of some genes of the enzymes responsible for virulence of Candida may also play a role in resistance to fluconazole.

  10. Candidiasis esofágica en pacientes con sida. Estudio clínico y microbiológico

    Zenia Flores de Apodaca Verdura

    1998-08-01

    Full Text Available Se estudiaron 18 pacientes con sida, que presentaban síntomas gastrointestinales altos o lesiones en la cavidad oral sugestivos de candidiasis. Se obtuvieron datos clínicos, muestras de la cavidad oral, biopsias y cepillado esofágico, así como suero de todos los pacientes. El síntoma que principalmente se observó en la candidiasis esofágica fue la disfagia; la candidiasis oral se comportó de forma asintomática. Candida albicans fue la especie que más se aisló con predominio del serotipo A. Se analizaron por la técnica de electroforesis en gel de poliacrilamida los perfiles proteicos de las 12 cepas pertenecientes a esta especie, se observó identidad de sus patrones de peso molecular, lo que sugiere que sea la misma cepa la que se halla en la cavidad oral y en el esófago. Se evaluó la respuesta de anticuerpos anti-C. albicans, no resultó útil la inmunodifusión doble para el diagnóstico de candidiasis esofágica.18 AIDS patients who presented high gastrointestinal symptoms or lesions in the oral cavity suggestive of candidiasis were studied. Clinical data, specimen of the oral cavity, biopsies and esophageal brushing, as well as serum from all patients were obtained. Dysphagia was the main symptom observed in the esophageal candidiasis. Candida albicans was the most isolated species with a predominance of serotype A. The protein profiles of 12 strains belonging to this species were analyzed by the polyacrylamide gel electrophoresis, and it was found that their molecular weight patterns were identical, which indicates that the same strain is in the oral cavity and in the esophagus. The response of the anti-C. albicans antibodies was evaluated. The double immunodiffussion was not useful for the diagnosis of esophageal candidiasis.

  11. Pseudomembranous Type of Oral Candidiasis is Associated with Decreased Salivary Flow Rate and Secretory Immunoglobulin A Levels.

    Mahajan, Bela; Bagul, Neeta; Desai, Rajiv; Reddy, Mamatha; Mahajan, Amit; Shete, Ashwini; Risbud, Arun; Mane, Arati

    2015-08-01

    Saliva plays an important role in maintaining microbial homeostasis in the oral cavity, while salivary gland hypofunction predisposes the oral mucosa to pathologic alteration and increases the risk for oral candidiasis. This study sought to determine the salivary flow rate (SFR) and secretory immunoglobulin A (SIgA) levels in HIV-positive and HIV-negative individuals and evaluate their relationship with the determinants of oral candidiasis. Sixty HIV-positive (30 with and 30 without oral candidiasis) and 30 healthy HIV-negative individuals were enrolled. Cotton pellet was weighed pre- and post-saliva collection for the assessment of SFR, while SIgA levels were estimated by commercial ELISA (Diametra, Italy) kit. The mean ± SD, SFR and SIgA levels in HIV-positive individuals with candidiasis, without candidiasis and HIV-negative controls were 0.396 ± 0.290, 0.546 ± 0.355 and 0.534 ± 0.214 ml/min and 115.891 ± 37.621, 136.024 ± 51.075 and 149.418 ± 31.765 µg/ml, respectively. A positive correlation between low CD4 counts (indicator of immunodeficiency) and SIgA was observed in HIV-positive individuals with candidiasis (r = 0.373, p = 0.045). We also report here for the first time the significant decrease in SFR and SIgA levels in individuals presenting with pseudomembranous type of oral candidiasis and Candida albicans infection. PMID:25682023

  12. A randomized phase III prospective trial of bethanechol to prevent mucositis, candidiasis, and taste loss in patients with head and neck cancer undergoing radiotherapy. A secondary analysis

    The aim of this study was to determine the impact of bethanechol administration concomitant to radiotherapy (RT) on oral mucositis, candidiasis and taste loss. We performed a secondary analysis of a previously conducted prospective randomized trial which evaluated the effect of bethanechol on salivary gland dysfunction before, during, and after RT for head and neck cancer (HNC), in comparison to artificial saliva. Mucositis, candidiasis and taste loss were analyzed in 36 patients. Mucositis was scored using the World Health Organization (WHO) method; candidiasis was diagnosed by means of clinical examination, whereas taste loss was assessed by the patients' subjective report of absence of taste. No significant differences were observed between groups in relation to frequency and severity of mucositis or frequency of candidiasis and taste loss. In conclusion, bethanechol does not appear to reduce the incidence of mucositis, candidiasis, and taste loss when administered during RT. (author)

  13. Treatment of asymptomatic vaginal candidiasis in pregnancy to prevent preterm birth: an open-label pilot randomized controlled trial

    Rickard Kristen

    2011-03-01

    Full Text Available Abstract Background Although the connection between ascending infection and preterm birth is undisputed, research focused on finding effective treatments has been disappointing. However evidence that eradication of Candida in pregnancy may reduce the risk of preterm birth is emerging. We conducted a pilot study to assess the feasibility of conducting a large randomized controlled trial to determine whether treatment of asymptomatic candidiasis in early pregnancy reduces the incidence of preterm birth. Methods We used a prospective, randomized, open-label, blinded-endpoint (PROBE study design. Pregnant women presenting at Candida were randomized to 6-days of clotrimazole vaginal pessaries (100mg or usual care (screening result is not revealed, no treatment. The primary outcomes were the rate of asymptomatic vaginal candidiasis, participation and follow-up. The proposed primary trial outcome of spontaneous preterm birth Results Of 779 women approached, 500 (64% participated in candidiasis screening, and 98 (19.6% had asymptomatic vaginal candidiasis and were randomized to clotrimazole or usual care. Women were not inconvenienced by participation in the study, laboratory testing and medication dispensing were problem-free, and the follow-up rate was 99%. There was a tendency towards a reduction in spontaneous preterm birth among women with asymptomatic candidiasis who were treated with clotrimazole RR = 0.33, 95%CI 0.04-3.03. Conclusions A large, adequately powered, randomized trial of clotrimazole to prevent preterm birth in women with asymptomatic candidiasis is both feasible and warranted. Trial registration Australia and New Zealand Clinical Trials Register (ANZCTR: ACTRN12609001052224

  14. Prevalence, Risk Factors, and Clinical Findings of Candidiasis and Trichomoniasis in Women Supported by Selected Health Centers of Tabriz, Iran

    Sehhatie-Shafaie Fahimeh

    2014-10-01

    Full Text Available Objective: Vulvovaginitis candidiasis and trichomoniasis constitute at least 50% of infectious vaginitis cases. The aim of this study was to determine the prevalence, risk factors, and clinical findings of candidiasis and trichomoniasis in women supported by selected health centers of Tabriz, Iran. Materials and Methods: This was a cross-sectional study, in which 1000 women who had the study criteria were selected by random sampling. In addition, 12 health centers of Tabriz were selected for this study. A questionnaire was used to obtain their personal and reproductive information, checklist for clinical observations, and culture methods (sabouraud dextrose agar and diamond for diagnose vaginal infections. Results: The prevalence of candidiasis and trichomoniasis were 25.2 and 9.2%, respectively. Findings showed that candidiasis infection, history of diseases, vaginal pH, number of coitus, number of delivery, and number of vaginal delivery, breast feeding status, method of last delivery, and contraceptive methods are risk factors for candidacies. Moreover, age at marriage, personal health, sexual hygiene, and vaginal pH are risk factors for trichomoniasis. A statistically significant relationship was observed between candidiasis and clinical findings, such as pruritus, pruritus during coitus, burning sensation with coitus, dysuria in woman and her husband, dyspareunia, low abdominal pain, urinal symptoms, vaginal status, amount of discharge, consistency appearance, and color of discharges. Furthermore, a significant relationship was observed between trichomoniasis and dysuria, and appearance and color of vaginal discharge. Conclusion: Due to the high prevalence of candidiasis, trichomoniasis infections, and infected women as asymptomatic carriers, it seems necessary to pay more attention to these infections and make efforts for their prevention.

  15. EFFICACY OF SINGLE ORAL DOSE 150 mg FLUCONAZOLE IN TREATMENT OF VAGINAL CANDIDIASIS

    Shabana

    2013-10-01

    Full Text Available ABSTRACT: AIMS : This study aimed to find out the efficacy of single oral dose 150mg of fluconazole in treatment of acute vulvovaginal candidiasis, to e valuate its safety assessment and the clinical and mycological efficacy assessment. MATERIALS AND METHODS: T his study is carried out in department of obstetrics and gynaecology Gandhi medical college sultania hospital Bhopal and with the help of microbiolo gy department Gandhi medical college Bhopal over a period of one year. It is a hospital based clinical prospective study. RESULTS : Maximum age incidence was found between 21 - 30years. Mostly patients belonged to low socioeconomic status and were uneducated. Maximum patients were married (98% and multiparous (92%, nulliparous formed the smallest group (8%. In factors predisposing to candidiasis, contraceptive methods were found to be important in which maximum incidence was found in patients using oral con traception about 32% and 12% of IUCD users were affected. Other factors were antibiotic treatment (5% and diabetes (2%. Vaginal discharge and pruritis were the two commonest symptoms found. Among the signs vaginal discharge and white plaques was the comm onest sign. On follow up visits 88 cases had complete clinical cure and only 6 cases showed failure and 9 recurrence s . In mycological assessment maximum 135 cases showed complete cure, 6 were failure and 9 recurrence. In overall results, excellent results were found in 88cases, good in 38 cases, fair in 9 cases and recurrence in 9 cases. Recurrences were mainly due to rectal carriers. CONCLUSION: In co n clusion fluconazole was found effective as a systemic single oral dose therapy for acute vulvovaginal cand idiasis. It is proved safe in terms of tolerance and preferred by patients. So in view of its favourable patients acceptability and compliance profile, it is considered as a first line therapeutic choice for treatment of women with vaginal candidiasis.

  16. Prevalence of vulvovaginal candidiasis among nonpregnant women attending a tertiary health care facility in Abuja, Nigeria

    Emeribe AU

    2015-06-01

    Full Text Available Anthony Uchenna Emeribe,1 Idris Abdullahi Nasir,2 Justus Onyia,2 Alinwachukwu Loveth Ifunanya31Department of Medical Laboratory Science, University of Calabar, Calabar, Cross River State, Nigeria; 2Department of Medical Microbiology, University of Abuja Teaching Hospital, Gwagwalada, Federal Capital Territory, Nigeria; 3Department of Medical Laboratory, School of Health Technology, Tsafe, Zamfara State, NigeriaBackground: Candida spp. are normal flora of the vagina that eventually become pathogenic under some prevailing conditions, and thus present as a common etiology of vulvovaginitis. When prompt recognition and laboratory confirmation is not achieved, this could lead to devastating genital discomfort and a major reason for frequent hospital visits.Aims: This was a cross-sectional prospective study that aimed to determine the prevalence and some associated risk factors of vulvovaginal candidiasis (VVC among nonpregnant women attending University of Abuja Teaching Hospital, Gwagwalada.Subjects and methods: A pair of high vaginal swab and endocervical swab samples was collected from each of 200 individual participating subjects. They were separately inoculated on Sabouraud's dextrose agar and incubated aerobically at 33°C for 48 hours. Ten percent KOH wet mount and Gram staining was done on swabs and colonies, respectively. Structured questionnaires were used to obtain sociodemographic and clinical data.Results: Of the 200 participating subjects, the prevalence of Candida albicans was 6.5% and that of non-albicans candidiasis 7.5%. Candidiasis was observed mostly among the 20- to 30-year age-group. All subjects with Candida-positive culture had been on antibacterial therapy prior to participating in this study – 28 (100%. There was a statistical relationship between the prevalence of VVC with previous antibacterial therapy (P<0.05, but not with age or other prevailing health conditions studied (P>0.05.Conclusion: The outcome of this study

  17. Appropriateness and cost-effectiveness in the treatment of invasive candidiasis in Internal Medicine Wards

    Enrico Tagliaferri

    2014-12-01

    Full Text Available Invasive candidiasis (IC, including candidemia, is a major cause of morbidity and mortality among patients and the majority of cases of candidemia are documented in Medical Wards. Early identification of patients at risk, knowledge of local epidemiology and prompt efforts to define etiologic diagnosis are pivotal to ensure appropriateness. Start with an echinocandin and switch to fluconazole when possible, seems to represent a useful strategy for the management of IC. The choice between the three echinocandins should be based on the specific indications, pharmacokinetic/pharmacodynamic profile, clinical experience and cost.

  18. Photodynamic therapy as a new approach in vulvovaginal candidiasis in murine model

    Santi, Maria E.; Lopes, Rubia G.; Prates, Renato A.; Sousa, Aline; Ferreira, Luis R.; Fernandes, Adjaci U.; Bussadori, Sandra K.; Deana, Alessandro M.

    2015-02-01

    Vulvovaginal candidiasis is a common cause of vaginal infections. This study investigates the efficiency of antimicrobial photodynamic therapy (aPDT) against yeast cells in mice. Methylene blue (MB), malachite green (MG), and a special designed protoporphirin (PpNetNI) were used as photosensitizers. Female BALB-c mice were infected with Candida albicans ATCC 90028. PDT was applied with two different light sources, intravaginal and transabdominal. Vaginal washes were performed and cultivated for microbial quantification. Antimicrobial PDT was able to decrease microbial content with MB and PpNetNI (pcandidiasis.

  19. Ketoconazole hepatotoxicity in a patient treated for environmental illness and systemic candidiasis

    Brusko, C.S.; Marten, J.T. (Purdue University School of Pharmacy and Pharmacal Sciences, Lafayette, IN (United States))

    1991-12-01

    Environmental illness, a hypothesized disease caused by exposure to substances such as combustion products, pesticides, food additives, and Candida albicans, is discussed. The case of a patient with environmental illness and systemic candidiasis for six weeks with ketoconazole, liver enzyme concentrations increased. One month after discontinuation of ketoconazole, the liver enzyme concentrations decreased; however, over the next five months, liver enzymes and bilirubin increased. The patient developed encephalopathy and eventually was transferred to a medical center for possible liver transplant. A review of the literature pertaining to ketoconazole hepatotoxicity is also presented.16 references.

  20. Antifungal activity of the piroctone olamine in experimental intra-abdominal candidiasis

    do Couto, Fabíola Maria Marques; do Nascimento, Silene Carneiro; Júnior, Silvio Francisco Pereira; da Silva, Vanessa Karina Alves; Leal, André Ferraz Goiana; Neves, Rejane Pereira

    2016-01-01

    This work aimed to evaluate the antifungal activity of piroctone olamine in the treatment of intra-abdominal candidiasis in an experimental model using Swiss mice. The mice (n = 6) were infected by intraperitoneal injection of 0.2 ml of C. albicans (107cells/ml in saline). The animals were observed daily for clinical signs and mortality for 14 days. The treatment with piroctone olamine (0.5 mg/kg) was performed 72 h after infection by intraperitoneal administration. For comparison, a group of...

  1. TUBERCULOSIS INFECTION MIGHT INCREASE THE RISK OF INVASIVE CANDIDIASIS IN AN IMMUNOCOMPETENT PATIENT

    Xiao-Hua CHEN

    2015-06-01

    Full Text Available Deep Candida infections commonly occur in immunosuppressed patients. A rare case of a multiple deep organ infection with Candida albicans and spinal tuberculosis was reported in a healthy young man. The 19-year-old man complained of month-long fever and lower back pain. He also had a history of scalded mouth syndrome. Coinfection with Mycobacterium tuberculosis and Candida albicans was diagnosed using the culture of aspirates from different regions. Symptoms improved considerably after antifungal and antituberculous therapy. This case illustrates that infection with tuberculosis might impair the host's immune system and increase the risk of invasive candidiasis in an immunocompetent patient.

  2. Antifungal treatment with carvacrol and eugenol of oral candidiasis in immunosuppressed rats

    N. Chami

    2004-06-01

    Full Text Available Carvacrol and eugenol, the main (phenolic components of essential oils of some aromatic plants, were evaluated for their therapeutic efficacy in the treatment of experimental oral candidiasis induced by Candida albicans in immunosuppressed rats. This anticandidal activity was analyzed by microbiological and histopathological techniques, and it was compared with that of nystatin, which was used as a positive control. Microbiologically, carvacrol and eugenol significantly (p<0.05 reduced the number of colony forming units (CFU sampled from the oral cavity of rats treated for eight consecutive days, compared to untreated control rats. Treatment with nystatin gave similar results. Histologically, the untreated control animals showed numerous hyphae on the epithelium of the dorsal surface of the tongue. In contrast no hyphal colonization of the epithelium was seen in carvacrol-treated animals, while in rats treated with eugenol, only a few focalized zones of the dorsal surface of the tongue were occupied by hyphae. In the nystatin treated group, hyphae were found in the folds of the tongue mucosa. Thus, the histological data were confirmed by the microbiological tests for carvacrol and eugenol, but not for the nystatin-treated group. Therefore, carvacrol and eugenol could be considered as strong antifungal agents and could be proposed as therapeutic agents for oral candidiasis.

  3. Esophageal Stricture Secondary to Candidiasis in a Child with Glycogen Storage Disease 1b.

    Lee, Kyung Jae; Choi, Shin Jie; Kim, Woo Sun; Park, Sung-Sup; Moon, Jin Soo; Ko, Jae Sung

    2016-03-01

    Esophageal candidiasis is commonly seen in immunocompromised patients; however, candida esophagitis induced stricture is a very rare complication. We report the first case of esophageal stricture secondary to candidiasis in a glycogen storage disease (GSD) 1b child. The patient was diagnosed with GSD type 1b by liver biopsy. No mutation was found in the G6PC gene, but SLC37A4 gene sequencing revealed a compound heterozygous mutation (p.R28H and p.W107X, which was a novel mutation). The patient's absolute neutrophil count was continuously under 1,000/µL when he was over 6 years of age. He was admitted frequently for recurrent fever and infection, and frequently received intravenous antibiotics, antifungal agents. He complained of persistent dysphagia beginning at age 7 years. Esophageal stricture and multiple whitish patches were observed by endoscopy and endoscopic biopsy revealed numerous fungal hyphae consistent with candida esophagitis. He received esophageal balloon dilatation four times, and his symptoms improved. PMID:27066451

  4. Virgin Coconut Oil (VCO Enriched with Zn as Immunostimulator for Vaginal Candidiasis Patient

    HERY WINARSI

    2008-12-01

    Full Text Available Disturbance on the immune system and deficiency of Zn is two factors which often trigger vaginal candidiasis patient. The aim of the research was to study the effect of virgin coconut oil (VCO enriched with Zn to the amount of neutrophil and lymphocyte subset cells, and the level of IL-2 and IgG in vaginal candidiasis patient. Thirty women were grouped into three (ten women in each group: A, B and C, and intervened for two months. Women in A group were intervened with two tablespoon/day; those in B group were intervened with one tablespoon/day; while those in C group served as control (placebo. Blood was sampled at baseline time, one and two months after intervention. Hematological test by Micros-OT was done on a part of blood, and the plasma was used for IL-2 and IgG level tests using ELISA. The virgin coconut oil enriched with Zn maintained the number of neutrophil and NK cells, but increased Tc cells from 521 to 649 cells/mm3, increased Th cells from 1.090 to 1.380 cells/mm3. The enriched VCO also increase level of IL-2 from 0.25313 to 0.27337 pg/ml, while the IgG level changed from equivocal to negative. The recommended dosage was one tablespoon each day.

  5. Candida duobushaemulonii: an emerging rare pathogenic yeast isolated from recurrent vulvovaginal candidiasis in Brazil.

    Boatto, Humberto Fabio; Cavalcanti, Sarah Desirée Barbosa; Del Negro, Gilda Mb; Girão, Manoel João Bc; Francisco, Elaine Cristina; Ishida, Kelly; Gompertz, Olga Fischman

    2016-06-01

    The aim of this study was to identify Candida species isolated from women diagnosed with recurrent vulvovaginal candidiasis (RVVC) and their partners; and to evaluate the fluconazole (FLZ) susceptibility of the isolates. In a period of six years, among 172 patients diagnosed with vulvovaginal candidiasis, 13 women that presented RVVC and their partners were selected for this investigation. The isolates were obtained using Chromagar Candida medium, the species identification was performed by phenotypic and molecular methods and FLZ susceptibility was evaluated by E-test. Among 26 strains we identified 14 Candida albicans, six Candida duobushaemulonii, four Candida glabrata, and two Candida tropicalis. Agreement of the isolated species occurred in 100% of the couples. FLZ low susceptibility was observed for all isolates of C. duobushaemulonii (minimal inhibitory concentration values from 8-> 64 µg/mL), two C. glabrata isolates were FLZ-resistant and all C. albicans and C. tropicalis isolates were FLZ-susceptible. This report emphasises the importance of accurate identification of the fungal agents by a reliable molecular technique in RVVC episodes besides the lower antifungal susceptibility profile of this rare pathogen C. duobushaemulonii to FLZ. PMID:27304096

  6. Effect of Streptococcus salivarius K12 on the in vitro growth of Candida albicans and its protective effect in an oral candidiasis model.

    Ishijima, Sanae A; Hayama, Kazumi; Burton, Jeremy P; Reid, Gregor; Okada, Masashi; Matsushita, Yuji; Abe, Shigeru

    2012-04-01

    Oral candidiasis is often accompanied by severe inflammation, resulting in a decline in the quality of life of immunosuppressed individuals and elderly people. To develop a new oral therapeutic option for candidiasis, a nonpathogenic commensal oral probiotic microorganism, Streptococcus salivarius K12, was evaluated for its ability to modulate Candida albicans growth in vitro, and its therapeutic activity in an experimental oral candidiasis model was tested. In vitro inhibition of mycelial growth of C. albicans was determined by plate assay and fluorescence microscopy. Addition of S. salivarius K12 to modified RPMI 1640 culture medium inhibited the adherence of C. albicans to the plastic petri dish in a dose-dependent manner. Preculture of S. salivarius K12 potentiated its inhibitory activity for adherence of C. albicans. Interestingly, S. salivarius K12 was not directly fungicidal but appeared to inhibit Candida adhesion to the substratum by preferentially binding to hyphae rather than yeast. To determine the potentially anti-infective attributes of S. salivarius K12 in oral candidiasis, the probiotic was administered to mice with orally induced candidiasis. Oral treatment with S. salivarius K12 significantly protected the mice from severe candidiasis. These findings suggest that S. salivarius K12 may inhibit the process of invasion of C. albicans into mucous surfaces or its adhesion to denture acrylic resins by mechanisms not associated with the antimicrobial activity of the bacteriocin. S. salivarius K12 may be useful as a probiotic as a protective tool for oral care, especially with regard to candidiasis. PMID:22267663

  7. CARD9 Deficiency and Spontaneous Central Nervous System Candidiasis: Complete Clinical Remission With GM-CSF Therapy

    Gavino, Christina; Cotter, Anthony; Lichtenstein, Daniel; Lejtenyi, Duncan; Fortin, Claude; Legault, Catherine; Alirezaie, Najmeh; Majewski, Jacek; Sheppard, Donald C; Behr, Marcel A.; Foulkes, William D.; Vinh, Donald C.

    2014-01-01

    We demonstrate autosomal-recessive CARD9 deficiency in a patient with relapsing Candida albicans meningoencephalitis. The novel, hypomorphic mutation impaired granulocyte-macrophage colony-stimulating factor (GM-CSF) but not Th17 responses. Adjunctive GM-CSF therapy resulted in clinical remission, suggesting that a CARD9/GM-CSF axis contributes to susceptibility to candidiasis.

  8. Speciation and antifungal susceptibility of esophageal candidiasis in cancer patients in a tertiary care hospital in South India

    J. Abirami Lakshmy

    2016-01-01

    Full Text Available Esophageal candidiasis is the most common opportunistic infection in patients with altered immunity such as Human Immunodeficiency Virus (HIV infection, cancer patients on chemotherapy and radiotherapy. Neutropenia, irradiation and chemotherapy will facilitate deeper mucosal invasion leading to esophageal candidiasis. Empirical treatment of esophageal candidiasis without antifungal susceptibility testing will lead to the emergence of drug resistant species increasing the morbidity and mortality associated with cancer. The present study aimed to study the frequency of esophageal candida in individuals with cancer, species level identification and antifungal susceptibility pattern. Scrapings of whitish appearing lesions were obtained from a total of thirty five cases of endoscopically identified esophageal candidiasis were obtained from cancer patients. Identification of the Candida isolates were done by cultivation in Sabouraud dextrose agar (SDA, Gram staining, germ tube test, colony morphology in Chrom agar and corn meal agar, sugar assimilation and fermentation tests. Antifungal susceptibility was done by Microbroth dilution method for Fluconazole, Itraconazole and Amphotericin B. We found that Candida albicans was the predominant species isolated followed by Candida tropicalis and Candida glabrata. Sensitivity rates were 94%, 96% and 100% for Fluconazole, Itraconazole and Amphotericin B. Species level identification of Candida isolated from esophageal candidiasis and their antifungal sensitivity testing should be performed for early identification of resistant strains and for promptly treating the cases there by preventing the dissemination of infection in case of immune-compromised individuals. Further the susceptibility pattern will facilitate therapeutic guidance especially in individuals prone to relapse. [J Med Allied Sci 2016; 6(1: 29-34

  9. Candidiasis (Thrush)

    ... immune system by taking antiretroviral medications (ARVs). Most candida infections are easily treated with local therapies. In people ... effects. Several natural therapies seem to help control candida infections. Back to Fact Sheet Categories The AIDS InfoNet ...

  10. Renal candidiasis

    Most fungal infections of the urinary tract are caused by Candida albicans, a yeast-like saprophytic fungus which may become apathogen under various conditions which lower the host resistance. The use of computed tomography in the diagnosis of renal fungus balls is the subject of this communication with emphasis on the radiologists role in the recognition of this entity. (H.W.). 6 refs.; 2 figs

  11. Candidiasis (oropharyngeal)

    Pankhurst, Caroline L

    2012-01-01

    Opportunistic infection with the fungus Candida albicans causes painful red or white lesions of the oropharynx, which can affect taste, speech, and the act of eating. Candida is present in the mouth of up to 60% of healthy people, but overt infection is associated with immunosuppression, diabetes, broad-spectrum antibiotics, corticosteroid use, haematinic deficiencies, and denture wear.

  12. Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China.

    Shi, Xiao-Yu; Yang, Yan-Ping; Zhang, Ying; Li, Wen; Wang, Jie-Di; Huang, Wen-Ming; Fan, Yi-Ming

    2015-04-01

    There is limited information regarding the molecular epidemiology and antifungal susceptibilities of Candida isolates using the Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18-50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and the D1/D2 domain. Antifungal susceptibility testing of seven antifungal agents was performed using the Neo-Sensitabs tablet diffusion method. Candida species were isolated from 186 cases (61.79 %). The most common pathogen was Candida albicans (91.4 %), followed by Candida glabrata (4.3 %), Candida tropicalis (3.2 %) and Candida parapsilosis (1.1 %). The susceptibility rates to C. albicans were higher for caspofungin, voriconazole and fluconazole than those for itraconazole, miconazole, ketoconazole and terbinafine (Ptreatment of VVC. PMID:25596116

  13. The use of cultures and immunologic procedures to predict oropharyngeal candidiasis in patients on steroid aerosols.

    Spector, S L; Wangaard, C; Bardana, E J

    1982-05-01

    Sixty-seven asthmatic individuals treated with either beclomethasone diproprionate or flunisolide were sequentially evaluated for up to 32 months to determine the incidence of oropharyngeal candidiasis as well as laboratory parameters which might be predictive of this complication. Throat cultures and measurements of Candida antibody by immunodiffusion and radioimmunoassay were performed and compared over time and treatment groups. Unlike other studies, pre-treatment Candida precipitins did not predict increased risk for clinical thrush nor did quantitative determinations of Candida antibody. Those patients with positive cultures pre-trial, however, had a significantly higher incidence of clinical thrush than those with negative cultures (P less than 0.01). No significant changes occurred over time or between drugs for any of the parameters. Symptomatic thrush, however, was slightly more common in those patients treated with beclomethasone. PMID:7105391

  14. Syngonanthus nitens Bong. (Rhul.)-Loaded Nanostructured System for Vulvovaginal Candidiasis Treatment.

    Dos Santos Ramos, Matheus Aparecido; de Toledo, Luciani Gaspar; Calixto, Giovana Maria Fioramonti; Bonifácio, Bruna Vidal; de Freitas Araújo, Marcelo Gonzaga; Dos Santos, Lourdes Campaner; de Almeida, Margarete Teresa Gottardo; Chorilli, Marlus; Bauab, Taís Maria

    2016-01-01

    Herbal-loaded drug delivery nanotechnological systems have been extensively studied recently. The antimicrobial activity of medicinal plants has shown better pharmacological action when such plants are loaded into a drug delivery system than when they are not loaded. Syngonanthus nitens Bong. (Rhul.) belongs to the Eriocaulaceae family and presents antiulcerogenic, antioxidant, antibacterial, and antifungal activity. The aim of this study was to evaluate the antifungal activity of Syngonanthus nitens (S. nitens) extract that was not loaded (E) or loaded (SE) into a liquid crystal precursor system (S) for the treatment of vulvovaginal candidiasis (VVC) with Candida albicans. The minimal inhibitory concentration (MIC) was determined by the microdilution technique. Additionally, we performed hyphae inhibition and biofilm tests. Finally, experimental candidiasis was evaluated in in vivo models with Wistar female rats. The results showed effective antifungal activity after incorporation into S for all strains tested, with MICs ranging from 31.2 to 62.5 μg/mL. Microscopic observation of SE revealed an absence of filamentous cells 24 h of exposure to a concentration of 31.2 μg/mL. E demonstrated no effective action against biofilms, though SE showed inhibition against biofilms of all strains. In the in vivo experiment, SE was effective in the treatment of infection after only two days of treatment and was more effective than E and amphotericin B. The S. nitens is active against Candida albicans (C. albicans) and the antifungal potential is being enhanced after incorporation into liquid crystal precursor systems (LCPS). These findings represent a promising application of SE in the treatment of VVC. PMID:27556451

  15. Candidiasis in Pediatrics; Identification and In vitro Antifungal Susceptibility of the Clinical Isolates

    Mohammadi, R; Ataei, B

    2016-01-01

    Background Candida species are normal microflora of oral cavity, vagina, and gastrointestinal tract. They are the third most prevalent cause of pediatric health care–associated bloodstream fungal infection. This study aimed to provide an epidemiological feature of candidiasis and also presents an antifungal susceptibility profile of clinical Candida isolates among children. Materials and Methods During July 2013 to February 2015, 105 patients from different hospitals of Isfahan, Iran, were examined for candidiasis by phenotypic tests. Samples were obtained from nail clippings, blood, thrush, BAL, urine, oropharynx, skin, and eye discharge. The age range of patients was between 18 days to 16 years. Genomic DNA of isolates was extracted and ITS1-5.8SrDNA-ITS2 region was amplified by ITS1 and ITS2 primers. The PCR products were digested using the restriction enzyme MspI. Minimum inhibitory concentration (MICs) was determined using microdilution broth method according to the clinical and laboratory standards institute (CLSI) M27-A3 and M27-S4 documents. Results Forty-three patients (40.9%) had Candida infection.The most clinical strains were isolated from nail infections (39.5%), and candidemia (13.9%). Candida albicans was the most prevalent species (46.5%). MICs ranges for amphotericin B, fluconazole, and itraconazole were (0.025-0.75 µg/ml), (0.125-16 µg/ml), and (0.094-2 µg/ml), respectively. Conclusion Due to high incidence of Candida infections among children, increasing of fatal infection like candidemia, and emersion of antifungal resistance Candida isolates, early and precise identification of the Candida species and determination of antifungal susceptibility patterns of clinical isolates may lead to better management of the infection. PMID:27222702

  16. Favorable outcome of hepatosplenic candidiasis in a patient with acute leukemia

    Čolović Nataša

    2015-01-01

    Full Text Available Introduction. Acute leukemias treatment requires strong chemotherapy. Patients that develop bone marrow aplasia become immunocompromised, thus becoming liable to bacterial and fungal infections. Fungal infections caused by Candida are frequent. Hepatosplenic candidiasis (HSC is a frequent consequence of invasive candidiasis which is clinically presented with prolonged febrility unresponsive to antibiotics. Case Outline. A 53-year-old patient with acute myeloid leukemia was submitted to standard chemotherapy “3+7” regimen (daunoblastine 80 mg i.v. on days 1 to 3, cytarabine 2Ч170 mg i.v. during 7 days and achieved complete remission. However, during remission he developed febrility unresponsive to antibiotics. Computerised tomography (CT of the abdomen showed multiple hypodense lesions within the liver and spleen. Haemocultures on fungi were negative. However, seroconversion of biomarkers for invasive fungal infection (IFI (Candida and Aspergillus antigen/Ag and antibody/Ab indicated possible HSC. Only high positivity of anti-Candida IgG antibodies, positivity of mannan and CT finding we regarded sufficient for the diagnosis and antimycotic therapy. Three months of treatment with different antimycotics were necessary for complete disappearance of both clinical symptoms and CT findings. Conclusion. In patients with prolonged febrile neutropenia IFI has to be strongly suspected. If imaging techniques show multiple hypodense lesions within liver and spleen, HSC has to be taken seriously into consideration. We believe that, along with CT finding, positive laboratory Candida biomarkers (mannan and IgG antibodies should be considered sufficient for “probable HSC” and commencement of antifungal therapy, which must be long enough, i.e. until complete disappearance of clinical symptoms and CT findings are achieved. [Projekat Ministarstva nauke Republike Srbije, br. OI 175034

  17. Syngonanthus nitens Bong. (Rhul.)-Loaded Nanostructured System for Vulvovaginal Candidiasis Treatment

    dos Santos Ramos, Matheus Aparecido; de Toledo, Luciani Gaspar; Calixto, Giovana Maria Fioramonti; Bonifácio, Bruna Vidal; de Freitas Araújo, Marcelo Gonzaga; dos Santos, Lourdes Campaner; de Almeida, Margarete Teresa Gottardo; Chorilli, Marlus; Bauab, Taís Maria

    2016-01-01

    Herbal-loaded drug delivery nanotechnological systems have been extensively studied recently. The antimicrobial activity of medicinal plants has shown better pharmacological action when such plants are loaded into a drug delivery system than when they are not loaded. Syngonanthus nitens Bong. (Rhul.) belongs to the Eriocaulaceae family and presents antiulcerogenic, antioxidant, antibacterial, and antifungal activity. The aim of this study was to evaluate the antifungal activity of Syngonanthus nitens (S. nitens) extract that was not loaded (E) or loaded (SE) into a liquid crystal precursor system (S) for the treatment of vulvovaginal candidiasis (VVC) with Candida albicans. The minimal inhibitory concentration (MIC) was determined by the microdilution technique. Additionally, we performed hyphae inhibition and biofilm tests. Finally, experimental candidiasis was evaluated in in vivo models with Wistar female rats. The results showed effective antifungal activity after incorporation into S for all strains tested, with MICs ranging from 31.2 to 62.5 μg/mL. Microscopic observation of SE revealed an absence of filamentous cells 24 h of exposure to a concentration of 31.2 μg/mL. E demonstrated no effective action against biofilms, though SE showed inhibition against biofilms of all strains. In the in vivo experiment, SE was effective in the treatment of infection after only two days of treatment and was more effective than E and amphotericin B. The S. nitens is active against Candida albicans (C. albicans) and the antifungal potential is being enhanced after incorporation into liquid crystal precursor systems (LCPS). These findings represent a promising application of SE in the treatment of VVC. PMID:27556451

  18. Innovation of natural essential oil-loaded Orabase for local treatment of oral candidiasis

    Labib GS

    2015-06-01

    Full Text Available Gihan S Labib,1,2 Hibah Aldawsari1 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 2Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt Purpose: Oral candidiasis may be manifested in the oral cavity as either mild or severe oral fungal infection. This infection results from the overgrowth of Candida species normally existing in the oral cavity in minute amounts based on many predisposing factors. Several aspects have spurred the search for new strategies in the treatment of oral candidiasis, among which are the limited numbers of new antifungal drugs developed in recent years. Previous studies have shown that thyme and clove oils have antimycotic activities and have suggested their incorporation into pharmaceutical preparations. This study aimed to investigate the possibility of the incorporation and characterization of essential oils or their extracted active ingredients in Orabase formulations. Methods: Orabase loaded with clove oil, thyme oil, eugenol, and thymol were prepared and evaluated for their antifungal activities, pH, viscosity, erosion and water uptake characteristics, mechanical properties, in vitro release behavior, and ex vivo mucoadhesion properties. Results: All prepared bases showed considerable antifungal activity and acceptable physical characteristics. The release pattern from loaded bases was considerably slow for all oils and active ingredients. All bases showed appreciable adhesion in the in vitro and ex vivo studies. Conclusion: The incorporation of essential oils in Orabase could help in future drug delivery design, with promising outcomes on patients’ well-being. Keywords: antifungal activity, clove oil, eugenol, mucoadhesion, oral gel, thyme oil, thymol

  19. Microbiological screening of Irish patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy reveals persistence of Candida albicans strains, gradual reduction in susceptibility to azoles, and incidences of clinical signs of oral candidiasis without culture evidence.

    McManus, Brenda A

    2011-05-01

    Patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) are prone to chronic mucocutaneous candidiasis, which is often treated with azoles. The purpose of this study was to characterize the oral Candida populations from 16 Irish APECED patients, who comprise approximately half the total number identified in Ireland, and to examine the effect of intermittent antifungal therapy on the azole susceptibility patterns of Candida isolates. Patients attended between one and four clinical evaluations over a 5-year period, providing oral rinses and\\/or oral swab samples each time. Candida was recovered from 14\\/16 patients, and Candida albicans was the only Candida species identified. Interestingly, clinical diagnosis of candidiasis did not correlate with microbiological evidence of Candida infection at 7\\/22 (32%) clinical assessments. Multilocus sequence typing analysis of C. albicans isolates recovered from the same patients on separate occasions identified the same sequence type each time. Fluconazole resistance was detected in isolates from one patient, and isolates exhibiting a progressive reduction in itraconazole and\\/or fluconazole susceptibility were identified in a further 3\\/16 patients, in each case correlating with the upregulation of CDR- and MDR-encoded efflux pumps. Mutations were also identified in the ERG11 and the TAC1 genes of isolates from these four patients; some of these mutations have previously been associated with azole resistance. The findings suggest that alternative Candida treatment options, other than azoles such as chlorhexidine, should be considered in APECED patients and that clinical diagnosis of oral candidiasis should be confirmed by culture prior to the commencement of anti-Candida therapy.

  20. Microbiological screening of Irish patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy reveals persistence of Candida albicans strains, gradual reduction in susceptibility to azoles, and incidences of clinical signs of oral candidiasis without culture evidence.

    McManus, Brenda A; McGovern, Eleanor; Moran, Gary P; Healy, Claire M; Nunn, June; Fleming, Pádraig; Costigan, Colm; Sullivan, Derek J; Coleman, David C

    2011-05-01

    Patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) are prone to chronic mucocutaneous candidiasis, which is often treated with azoles. The purpose of this study was to characterize the oral Candida populations from 16 Irish APECED patients, who comprise approximately half the total number identified in Ireland, and to examine the effect of intermittent antifungal therapy on the azole susceptibility patterns of Candida isolates. Patients attended between one and four clinical evaluations over a 5-year period, providing oral rinses and/or oral swab samples each time. Candida was recovered from 14/16 patients, and Candida albicans was the only Candida species identified. Interestingly, clinical diagnosis of candidiasis did not correlate with microbiological evidence of Candida infection at 7/22 (32%) clinical assessments. Multilocus sequence typing analysis of C. albicans isolates recovered from the same patients on separate occasions identified the same sequence type each time. Fluconazole resistance was detected in isolates from one patient, and isolates exhibiting a progressive reduction in itraconazole and/or fluconazole susceptibility were identified in a further 3/16 patients, in each case correlating with the upregulation of CDR- and MDR-encoded efflux pumps. Mutations were also identified in the ERG11 and the TAC1 genes of isolates from these four patients; some of these mutations have previously been associated with azole resistance. The findings suggest that alternative Candida treatment options, other than azoles such as chlorhexidine, should be considered in APECED patients and that clinical diagnosis of oral candidiasis should be confirmed by culture prior to the commencement of anti-Candida therapy. PMID:21367996

  1. Lactic acid bacteria colonization and clinical outcome after probiotic supplementation in conventionally treated bacterial vaginosis and vulvovaginal candidiasis.

    Ehrström, Sophia; Daroczy, Katalin; Rylander, Eva; Samuelsson, Carolina; Johannesson, Ulrika; Anzén, Bo; Påhlson, Carl

    2010-09-01

    This randomized double-blind placebo controlled study assessed the vaginal colonization of lactic acid bacteria and clinical outcome. Vaginal capsules containing L gasseri LN40, Lactobacillus fermentum LN99, L. casei subsp. rhamnosus LN113 and P. acidilactici LN23, or placebos were administered for five days to 95 women after conventional treatment of bacterial vaginosis and/or vulvovaginal candidiasis. Vulvovaginal examinations and vaginal samplings were performed before and after administration, after the first and second menstruation, and after six months. Presence of LN strains was assessed using RAPD analysis. LN strains were present 2-3 days after administration in 89% of the women receiving LN strains (placebo: 0%, p vulvovaginal candidiasis lead to vaginal colonization, somewhat fewer recurrences and less malodorous discharge. PMID:20472091

  2. Antifungal susceptibility and molecular typing of 115 Candida albicans isolates obtained from vulvovaginal candidiasis patients in 3 Shanghai maternity hospitals.

    Ying, Chunmei; Zhang, Hongju; Tang, Zhenhua; Chen, Huifen; Gao, Jing; Yue, Chaoyan

    2016-05-01

    In our multicenter study, we studied the distribution ofCandidaspecies in vulvovaginal candidiasis patients and investigated antifungal susceptibility profile and genotype ofCandida albicansin vaginal swab. A total of 115Candida albicansstrains were detected in 135 clinical isolates. Minimum inhibitory concentration determinations showed that 83% and 81% of the 115Candida albicansstrains were susceptible to fluconazole and voriconazole. Randomly amplified polymorphic DNA analysis (RAPD) was applied to identify clonally related isolates from different patients at the local level. All tested strains were classified into genotype A (77.4%), genotype B (18.3%), and genotype C (4.3%). Genotype A was further classified into five subtypes and genotype B into two subtypes.Candida albicanswas the dominant pathogen of vulvovaginal candidiasis, the majority belonging to genotype A in this study. Exposure to azoles is a risk factor for the emergence of azole resistance amongCandida albicansisolated from VVC patients. PMID:26468549

  3. Physiologic Expression of the Candida albicans Pescadillo Homolog Is Required for Virulence in a Murine Model of Hematogenously Disseminated Candidiasis

    Uppuluri, Priya; Chaturvedi, Ashok K.; Jani, Niketa; Pukkila-Worley, Read; Monteagudo Castro, Carlos; Mylonakis, Eleftherios; Köhler, Julia R.; Lopez Ribot, Jose L.

    2012-01-01

    Morphogenetic conversions contribute to the pathogenesis of Candida albicans invasive infections. Many studies to date have convincingly demonstrated a link between filamentation and virulence; however, relatively little is known regarding the role of the filament-to-yeast transition during the pathogenesis of invasive candidiasis. We previously identified the C. albicans pescadillo homolog (PES1) as essential during yeast growth and growth of lateral yeast on hyphae but not during hyphal gro...

  4. Essential oil of Melaleuca alternifolia for the treatment of oral candidiasis induced in an immunosuppressed mouse model

    de Campos Rasteiro, Vanessa Maria; da Costa, Anna Carolina Borges Pereira; Araújo, Cássia Fernandes; de Barros, Patrícia Pimentel; Rossoni, Rodnei Dennis; Anbinder, Ana Lia; Jorge, Antonio Olavo Cardoso; Junqueira, Juliana Campos

    2014-01-01

    Background The search for alternative therapies for oral candidiasis is a necessity and the use of medicinal plants seems to be one of the promising solutions. The objective of this study was to evaluate the in vitro and in vivo effects of the essential oil of Melaleuca alternifolia on Candida albicans. Methods The minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of M. alternifolia were determined by the broth microdilution assay. For the in vivo stu...

  5. CARD9 deficiency and spontaneous central nervous system candidiasis: complete clinical remission with GM-CSF therapy.

    Gavino, Christina; Cotter, Anthony; Lichtenstein, Daniel; Lejtenyi, Duncan; Fortin, Claude; Legault, Catherine; Alirezaie, Najmeh; Majewski, Jacek; Sheppard, Donald C; Behr, Marcel A; Foulkes, William D; Vinh, Donald C

    2014-07-01

    We demonstrate autosomal-recessive Caspase Recruitment Domain-containing protein 9 (CARD9) deficiency in a patient with relapsing C. albicans meningoencephalitis. We identified a novel, hypomorphic mutation with intact Th17 responses, but impaired GM-CSF responses. We report complete clinical remission with adjunctive GM-CSF therapy, suggesting that a CARD9/GM-CSF axis contributes to susceptibility to candidiasis. PMID:24704721

  6. Activation of murine invariant NKT cells promotes susceptibility to candidiasis by IL-10 induced modulation of phagocyte antifungal activity.

    Haraguchi, Norihiro; Kikuchi, Norihiro; Morishima, Yuko; Matsuyama, Masashi; Sakurai, Hirofumi; Shibuya, Akira; Shibuya, Kazuko; Taniguchi, Masaru; Ishii, Yukio

    2016-07-01

    Invariant NKT (iNKT) cells play an important role in a variety of antimicrobial immune responses due to their ability to produce high levels of immune-modulating cytokines. Here, we investigated the role of iNKT cells in host defense against candidiasis using Jα18-deficient mice (Jα18(-/-) ), which lack iNKT cells. Jα18(-/-) mice were more resistant to the development of lethal candidiasis than wild-type (WT) mice. In contrast, treatment of WT mice with the iNKT cell activating ligand α-galactosylceramide markedly enhanced their mortality after infection with Candida albicans. Serum IL-10 levels were significantly elevated in WT mice in response to infection with C. albicans. Futhermore, IL-10 production increased after in vitro coculture of peritoneal macrophages with iNKT cells and C. albicans. The numbers of peritoneal macrophages, the production of IL-1β and IL-18, and caspase-1 activity were also significantly elevated in Jα18(-/-) mice after infection with C. albicans. The adoptive transfer of iNKT cells or exogenous administration of IL-10 into Jα18(-/-) reversed susceptibility to candidiasis to the level of WT mice. These results suggest that activation of iNKT cells increases the initial severity of C. albicans infection, most likely mediated by IL-10 induced modulation of macrophage antifungal activity. PMID:27151377

  7. Factors associated with time free of oral candidiasis in children living with HIV/AIDS, São Paulo, Brazil

    Thais Claudia Roma de Oliveira Konstantyner

    2013-11-01

    Full Text Available In clinical practice, recurrence of thrush is common in children living with HIV/AIDS. The aim of this study was to determine the factors associated with time spent free of oral candidiasis using survival analysis for recurrent events. A retrospective cohort study was carried out with 287 children treated between 1985 and 2009 at a reference center in the city of São Paulo, Brazil. The Prentice, Williams and Peterson model for recurrent events was used for the investigation of factors associated with the time free of oral candidiasis. The following factors were associated with the time patients were free of oral candidiasis: moderate immunodepression (HR = 2.5; p = 0.005, severe immunodepression (HR = 3.5; p < 0.001, anemia (HR = 3.3; p < 0.001, malnutrition (HR = 2.6; p = 0.004, hospitalization (HR = 2.2; p < 0.001, monotherapy (HR = 0.5; p = 0.006, dual therapy (HR = 0.3; p < 0.001 and triple therapy/highly active antiretroviral therapy (HR = 0.1; p < 0.001. The method analyzed in the present study proved useful for the investigation of recurrent events in patients living with HIV/AIDS.

  8. Study of risk factors and prevalence of invasive candidiasis in a tertiary care hospital

    Chowta Mukta

    2007-01-01

    Full Text Available Introduction: The frequency of invasive mycoses has increased dramatically during the past two decades owing to medical advances such as intensive cancer chemotherapy, broad-spectrum antimicrobial therapy, invasive medical devices, organ transplantation, human immunodeficiency virus (HIV disease epidemic and an expanding aging population. There were few Indian studies regarding the incidence and risk factors for candidemia. Hence the aim of this work was to evaluate the changes in the prevalence of candidemia and invasive candidiasis in a tertiary care hospital and also to assess the risk factors and predictors of mortality Materials and Methods: Nonsystematic review of patients with candidemia/invasive candidiasis was done during the period 1999 to 2004. All in-patients who had shown signs and symptoms of nosocomial blood stream infection were screened for candidial infection. Among these, 29 patients had candidemia/invasive candidiasis. Demographic and clinical data of these patients were recorded on a standardized form, which included age, sex, site of isolation, infectious diagnosis, underlying conditions, predisposing factors, catheter status and clinical outcome. The data were collected during the years of 1999 to 2004, which is divided into two time periods (1999-2001 and 2002-2004. Data collected during these different time spans are compared with each other. Results: A total of 255 patients were screened during the study period. Among these, 100 patients were screened during the period 1999-2001 and 155 patients were screened during the year 2002-2004. Out of these patients, 29 showed positive cultures in blood or other sterile site (ascitic fluid, bronchial aspirate and urine from suprapubic puncture. Out of these, 24 were males and five were females. The most common risk factor was use of intravenous canulae (62.1%, followed by prolonged use of antibiotics (34.5% and HIV infection (24.1%. There were no statistically significant

  9. A pseudo-randomised clinical trial of in situ gels of fluconazole for the treatment of oropharngeal candidiasis

    Shetty Veena A

    2011-04-01

    Full Text Available Abstract Background Oropharyngeal candidasis is a common opportunistic infection seen in immunocompromised patients. Fluconazole has a broad spectrum antifungal activity including a wide variety of candida species. Aim of the present investigation was to formulate and find out the relative efficacy of in situ gels of fluconazole. Method The in situ gels were prepared using polymers which exhibited sol-to-gel phase transition due to change in specific physico-chemical parameters, such as ion triggered system using gellan gum (0.5% w/v along with sodium carboxylmethylcellulose (0.35%w/v. The study design was bicenter, 'pseudo-randomised, single blind trial conducted in Mangalore., India, which includes 15 HIV positive patients, 15 patients with partial or completes dentures, and 15 patients who were treated with (active control fluconazole tablets 100 mg/day for 14 days. Severity of disease was scored clinically before treatment and at clinical evaluations on day 3, 7, 14, 18, 21, 35, and 42. Semiquantitative microbiological cultures of oral swabs were also obtained on same days. Results All patients had mycological documented oropharyngeal candidiasis and were treated with fluconazole (0.5%w/v in situ gels for 14 days Severity of disease was scored clinically before treatment and at different predetermined time intervals along with semi quantitative culture of oral swabs. The clinical response rate showed 97% cure after 14 days in the treated with in situ gel. In comparison, the control group treated with fluconazole tablets showed 85% improvement in symptoms of oral candidiasis. The patients suffering from HIV infection showed relapse in oral candidiasis at the end of 21 days. The patients having oral candidiasis due to partial or complete dentures showed complete recovery and were free from signs and symptoms of oral candidiasis. Conclusions The in situ gel formulation of fluconazole was well tolerated with no severe adverse reaction and offers

  10. Demonstration of Therapeutic Equivalence of Fluconazole Generic Products in the Neutropenic Mouse Model of Disseminated Candidiasis

    Gonzalez, Javier M.; Rodriguez, Carlos A.; Zuluaga, Andres F.; Agudelo, Maria; Vesga, Omar

    2015-01-01

    Some generics of antibacterials fail therapeutic equivalence despite being pharmaceutical equivalents of their innovators, but data are scarce with antifungals. We used the neutropenic mice model of disseminated candidiasis to challenge the therapeutic equivalence of three generic products of fluconazole compared with the innovator in terms of concentration of the active pharmaceutical ingredient, analytical chemistry (liquid chromatography/mass spectrometry), in vitro susceptibility testing, single-dose serum pharmacokinetics in infected mice, and in vivo pharmacodynamics. Neutropenic, five week-old, murine pathogen free male mice of the strain Udea:ICR(CD-2) were injected in the tail vein with Candida albicans GRP-0144 (MIC = 0.25 mg/L) or Candida albicans CIB-19177 (MIC = 4 mg/L). Subcutaneous therapy with fluconazole (generics or innovator) and sterile saline (untreated controls) started 2 h after infection and ended 24 h later, with doses ranging from no effect to maximal effect (1 to 128 mg/kg per day) divided every 3 or 6 hours. The Hill’s model was fitted to the data by nonlinear regression, and results from each group compared by curve fitting analysis. All products were identical in terms of concentration, chromatographic and spectrographic profiles, MICs, mouse pharmacokinetics, and in vivo pharmacodynamic parameters. In conclusion, the generic products studied were pharmaceutically and therapeutically equivalent to the innovator of fluconazole. PMID:26536105

  11. Demonstration of Therapeutic Equivalence of Fluconazole Generic Products in the Neutropenic Mouse Model of Disseminated Candidiasis.

    Javier M Gonzalez

    Full Text Available Some generics of antibacterials fail therapeutic equivalence despite being pharmaceutical equivalents of their innovators, but data are scarce with antifungals. We used the neutropenic mice model of disseminated candidiasis to challenge the therapeutic equivalence of three generic products of fluconazole compared with the innovator in terms of concentration of the active pharmaceutical ingredient, analytical chemistry (liquid chromatography/mass spectrometry, in vitro susceptibility testing, single-dose serum pharmacokinetics in infected mice, and in vivo pharmacodynamics. Neutropenic, five week-old, murine pathogen free male mice of the strain Udea:ICR(CD-2 were injected in the tail vein with Candida albicans GRP-0144 (MIC = 0.25 mg/L or Candida albicans CIB-19177 (MIC = 4 mg/L. Subcutaneous therapy with fluconazole (generics or innovator and sterile saline (untreated controls started 2 h after infection and ended 24 h later, with doses ranging from no effect to maximal effect (1 to 128 mg/kg per day divided every 3 or 6 hours. The Hill's model was fitted to the data by nonlinear regression, and results from each group compared by curve fitting analysis. All products were identical in terms of concentration, chromatographic and spectrographic profiles, MICs, mouse pharmacokinetics, and in vivo pharmacodynamic parameters. In conclusion, the generic products studied were pharmaceutically and therapeutically equivalent to the innovator of fluconazole.

  12. A prospective observational study of vulvovagintis in pregnant women in Argentina, with special reference to candidiasis.

    Mucci, María J; Cuestas, María L; Cervetto, María M; Landaburu, María F; Mujica, María T

    2016-07-01

    To evaluate the frequency of yeast, bacteria or protozoa in pregnant women and to correlate the possible associations of these microorganisms and their relationships with vulvovaginitis (VV) and cervicitis. Vaginal specimens were collected and prepared for smears in microscope slides for the evaluation of yeast, Trichomonas vaginalis and bacteria. Samples were cultured in specific culture medium. Cervical specimens were used to investigate the presence of Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma hominis. We enrolled 210 pregnant women, aged 10-42 years old. Of them, 38.1% were symptomatic. Symptoms were most prevalent in the second and third trimesters of pregnancy coincident with a major prevalence of microorganisms. In this study, 39.5% of pregnant women had normal microbial biota and symptoms of VV due to non-infectious causes were observed (6.2%). The occurrence of vulvovaginal candidiasis was 25% and Candida albicans with a prevalence of 80.7% was the dominant species (P = 0.005) while non-albicans Candida species and other yeast were more common in asymptomatic ones (P = 0.0038). The frequency of bacterial vaginosis, T. vaginalis, C. trachomatis and N. gonorrhoeae were 18.1%, 1.4, 1.4% and 0.5% respectively. PMID:26931504

  13. Evaluation of Mucoadhesive Gels with Propolis (EPP-AF in Preclinical Treatment of Candidiasis Vulvovaginal Infection

    Andresa Aparecida Berretta

    2013-01-01

    Full Text Available Vulvovaginal candidiasis is the second cause of vaginal infection in the USA. Clinical treatment of C. albicans infections is routinely performed with polyenes and azole derivatives. However, these drugs are responsible for undesirable side effects and toxicity. In addition, C. albicans azole and echinocandin resistance has been described. Propolis is a bee product traditionally used due to its antimicrobial, anti-inflammatory, and other properties. Therefore, the present work aimed to evaluate different propolis presentations in order to evaluate their in vitro and in vivo efficacy. The methodologies involved antifungal evaluation, chemical analysis, and the effects of the rheological and mucoadhesive properties of propolis based gels. The obtained results demonstrated the fungicide action of propolis extracts against all three morphotypes (yeast, pseudohyphae, and hyphae studied. The highest level of fungal cytotoxicity was reached at 6–8 hours of propolis cell incubation. Among the based gel formulations developed, the rheological and mucoadhesive results suggest that propolis based carbopol (CP1% and chitosan gels were the most pseudoplastic ones. CP1% was the most mucoadhesive preparation, and all of them presented low thixotropy. Results of in vivo efficacy demonstrated that propolis based gels present antifungal action similar to clotrimazole cream, suggesting that future clinical studies should be performed.

  14. Antifungal Activity of Brazilian Propolis Microparticles against Yeasts Isolated from Vulvovaginal Candidiasis

    Kelen Fátima Dalben Dota

    2011-01-01

    Full Text Available Propolis, a resinous compound produced by Apis mellifera L. bees, is known to possess a variety of biological activities and is applied in the therapy of various infectious diseases. The aim of this study was to evaluate the in vitro antifungal activity of propolis ethanol extract (PE and propolis microparticles (PMs obtained from a sample of Brazilian propolis against clinical yeast isolates of importance in the vulvovaginal candidiasis (VVC. PE was used to prepare the microparticles. Yeast isolates (n=89, obtained from vaginal exudates of patients with VVC, were exposed to the PE and the PMs. Moreover, the main antifungal drugs used in the treatment of VVC (Fluconazole, Voriconazole, Itraconazole, Ketoconazole, Miconazole and Amphotericin B were also tested. Minimum inhibitory concentration (MIC was determined according to the standard broth microdilution method. Some Candida albicans isolates showed resistance or dose-dependent susceptibility for the azolic drugs and Amphotericin B. Non-C. albicans isolates showed more resistance and dose-dependent susceptibility for the azolic drugs than C. albicans. However, all of them were sensitive or dose-dependent susceptible for Amphotericin B. All yeasts were inhibited by PE and PMs, with small variation, independent of the species of yeast. The overall results provided important information for the potential application of PMs in the therapy of VVC and the possible prevention of the occurrence of new symptomatic episodes.

  15. Prevalence and Treatment Management of Oropharyngeal Candidiasis in Cancer Patients: Results of the French Candidoscope Study

    Purpose: The aim of this pharmaco-epidemiological study was to evaluate the prevalence of oropharyngeal candidiasis (OPC) in cancer patients treated with chemotherapy and/or radiotherapy. Methods and Materials: Signs and symptoms of OPC were noted for all patients. Antifungal therapeutic management was recorded in OPC patients. Patients receiving local antifungal treatments were monitored until the end of treatment. Results: Enrolled in the study were 2,042 patients with solid tumor and/or lymphoma treated with chemotherapy and/or another systemic cancer treatment and/or radiotherapy. The overall prevalence of OPC was 9.6% (95% confidence interval, 8.4%-11.0%] in this population. It was most frequent in patients treated with combined chemoradiotherapy (22.0%) or with more than two cytotoxic agents (16.9%). Local antifungal treatments were prescribed in 75.0% of OPC patients as recommended by guidelines. The compliance to treatment was higher in patients receiving once-daily miconazole mucoadhesive buccal tablet (MBT; 88.2%) than in those treated with several daily mouthwashes of amphotericin B (40%) or nystatin (18.8%). Conclusion: OPC prevalence in treated cancer patients was high. Local treatments were usually prescribed as per guidelines. Compliance to local treatments was better with once-daily drugs.

  16. Biochemical analysis and application of molecular display technology on Candida albicans for diagnosing and preventing candidiasis.

    Shibasaki, Seiji; Aoki, Wataru; Ueda, Mitsuyoshi

    2013-01-01

    Medical facilities and advances in therapeutics have improved world over in recent times. Concomitant with this, the human population has been growing steadily. However, emerging infectious diseases such as severe acute respiratory syndrome (SARS) and AIDS, as well as re-emerging infectious diseases such as Japanese encephalitis and dengue fever, have been spreading in recent times. Three major infectious diseases, namely AIDS, malaria, and tuberculosis, are killing around 8 million people in the world annually. Although drugs effective against these infectious diseases are available at present, drastic therapeutics have not been developed yet. In addition, vaccines against these diseases often cannot prevent infections, because pathogenic viruses or bacteria evade the immune system of the host. Many diseases and emerging infections of pathogenic bacteria cannot be controlled by conventional pharmaceutics. These pathogens secrete regulatory factors. When the produced regulatory factor attains a certain level, an active factor is then produced by the pathogen to destroy the host. Considering these phenomena, we thought investigating characteristic regulatory or active factors will pave the way for developing novel vaccines or diagnostic drugs. Therefore, candidiasis was selected as a model, and application of the secretory protease of Candida albicans was examined for the development of novel drugs. Screening of novel candidates of antigens of C. albicans and vaccine development are also underway. In this paper, our strategy of platform technology against various infectious diseases are introduced. PMID:24189555

  17. Symptomatic vaginal candidiasis after pivmecillinam and norfloxacin treatment of acute uncomplicated lower urinary tract infection.

    Menday, A P

    2002-10-01

    The comparative incidence of symptomatic vaginal candidiasis associated with pivmecillinam and norfloxacin treatment in women with acute symptomatic uncomplicated UTI was determined in two randomised, double-blind, clinical trials. Adverse events reported following general enquiry were reviewed. Presence of Candida vaginitis was based upon the specification as such by investigators, the presence of specific symptoms such as genital pruritus and/or the prescription of specific anti Candida therapy. The incidences of Candida vaginitis were as follows; Study 1 pivmecillinam 200 mg tid for 7 days 13 (4.6%), pivmecillinam 200 mg bid for 7 days 7 (2.4%), pivmecillinam 400 mg bid for 3 days 6 (2.1%) and placebo 6 (2.1%), P=0.19. Study 2 pivmecillinam 400 mg bid for 3 days 7 (1.5%), norfloxacin 400 mg bid for 3 days 20 (4.3%), P=0.016. The incidence of Candida vaginitis in women with acute symptomatic uncomplicated UTI given 3 days treatment with pivmecillinam 400 mg bid is similar to that seen with placebo and is significantly less than the incidence with norfloxacin 400 mg bid for 3 days. PMID:12385688

  18. Antifungal activity of the piroctone olamine in experimental intra-abdominal candidiasis.

    do Couto, Fabíola Maria Marques; do Nascimento, Silene Carneiro; Júnior, Silvio Francisco Pereira; da Silva, Vanessa Karina Alves; Leal, André Ferraz Goiana; Neves, Rejane Pereira

    2016-01-01

    This work aimed to evaluate the antifungal activity of piroctone olamine in the treatment of intra-abdominal candidiasis in an experimental model using Swiss mice. The mice (n = 6) were infected by intraperitoneal injection of 0.2 ml of C. albicans (10(7)cells/ml in saline). The animals were observed daily for clinical signs and mortality for 14 days. The treatment with piroctone olamine (0.5 mg/kg) was performed 72 h after infection by intraperitoneal administration. For comparison, a group of animals (n = 6) was treated with amphotericin B (0.5 mg/kg). The mycological diagnosis was made by collecting the liver, spleen and kidneys. Data regarding the fungal growth and mortality were analyzed statistically by Student's t test and analysis of variance (ANOVA), with level of significance set at P piroctone olamine and amphotericin B) was statistically significant (P piroctone olamine and amphotericin B) was not statistically significant (P < 0.05). PMID:27119072

  19. Gene Polymorphisms in Pattern Recognition Receptors and Susceptibility to Idiopathic Recurrent Vulvovaginal Candidiasis

    Diana eRosentul

    2014-09-01

    Full Text Available Objective. Approximately 5% of women suffer from recurrent vulvovaginal candidiasis (RVVC. It has been hypothesized that genetic factors play an important role in the susceptibility to RVVC. The aim of this study was to assess the effect of genetic variants of genes encoding for Pattern Recognition Receptors (PRRs on susceptibility to RVVC.Study design. For the study, 119 RVVC patients and 263 healthy controls were recruited. Prevalence of polymorphisms in five PRRs involved in recognition of Candida were investigated in patients and controls. In silico and functional studies were performed to assess their functional effects. Results. Single nucleotide polymorphisms (SNPs in TLR1, TLR4, CLEC7A and CARD9 did not affect the susceptibility to RVVC. In contrast, a non-synonymous polymorphism in TLR2 (rs5743704, Pro631His increased the susceptibility to RVVC almost 3-fold. Furthermore, the TLR2 rs5743704 SNP had deleterious effects on protein function as assessed by in-silico analysis, and in-vitro functional assays suggested that it reduces production of IL-17 and IFN upon stimulation of peripheral blood mononuclear cells with C. albicans. No effects were observed on serum MBL concentrations.Conclusion. Genetic variation in TLR2 may significantly enhance susceptibility to RVVC by modulating host defense mechanisms against Candida. Additional studies are warranted to assess systematically the role of host genetic variation for susceptibility to RVVC.

  20. Signaling through IL-17C/IL-17RE is dispensable for immunity to systemic, oral and cutaneous candidiasis.

    Heather R Conti

    Full Text Available Candida albicans is a commensal fungal microbe of the human orogastrointestinal tract and skin. C. albicans causes multiple forms of disease in immunocompromised patients, including oral, vaginal, dermal and disseminated candidiasis. The cytokine IL-17 (IL-17A and its receptor subunits, IL-17RA and IL-17RC, are required for protection to most forms of candidiasis. The importance of the IL-17R pathway has been observed not only in knockout mouse models, but also in humans with rare genetic mutations that impact generation of Th17 cells or the IL-17 signaling pathway, including Hyper-IgE Syndrome (STAT3 or TYK2 mutations or IL17RA or ACT1 gene deficiency. The IL-17 family of cytokines is a distinct subclass of cytokines with unique structural and signaling properties. IL-17A is the best-characterized member of the IL-17 family to date, but far less is known about other IL-17-related cytokines. In this study, we sought to determine the role of a related IL-17 cytokine, IL-17C, in protection against oral, dermal and disseminated forms of C. albicans infection. IL-17C signals through a heterodimeric receptor composed of the IL-17RA and IL-17RE subunits. We observed that IL-17C mRNA was induced following oral C. albicans infection. However, mice lacking IL-17C or IL-17RE cleared C. albicans infections in the oral mucosa, skin and bloodstream at rates similar to WT littermate controls. Moreover, these mice demonstrated similar gene transcription profiles and recovery kinetics as WT animals. These findings indicate that IL-17C and IL-17RE are dispensable for immunity to the forms of candidiasis evaluated, and illustrate a surprisingly limited specificity of the IL-17 family of cytokines with respect to systemic, oral and cutaneous Candida infections.

  1. Signaling through IL-17C/IL-17RE is dispensable for immunity to systemic, oral and cutaneous candidiasis.

    Conti, Heather R; Whibley, Natasha; Coleman, Bianca M; Garg, Abhishek V; Jaycox, Jillian R; Gaffen, Sarah L

    2015-01-01

    Candida albicans is a commensal fungal microbe of the human orogastrointestinal tract and skin. C. albicans causes multiple forms of disease in immunocompromised patients, including oral, vaginal, dermal and disseminated candidiasis. The cytokine IL-17 (IL-17A) and its receptor subunits, IL-17RA and IL-17RC, are required for protection to most forms of candidiasis. The importance of the IL-17R pathway has been observed not only in knockout mouse models, but also in humans with rare genetic mutations that impact generation of Th17 cells or the IL-17 signaling pathway, including Hyper-IgE Syndrome (STAT3 or TYK2 mutations) or IL17RA or ACT1 gene deficiency. The IL-17 family of cytokines is a distinct subclass of cytokines with unique structural and signaling properties. IL-17A is the best-characterized member of the IL-17 family to date, but far less is known about other IL-17-related cytokines. In this study, we sought to determine the role of a related IL-17 cytokine, IL-17C, in protection against oral, dermal and disseminated forms of C. albicans infection. IL-17C signals through a heterodimeric receptor composed of the IL-17RA and IL-17RE subunits. We observed that IL-17C mRNA was induced following oral C. albicans infection. However, mice lacking IL-17C or IL-17RE cleared C. albicans infections in the oral mucosa, skin and bloodstream at rates similar to WT littermate controls. Moreover, these mice demonstrated similar gene transcription profiles and recovery kinetics as WT animals. These findings indicate that IL-17C and IL-17RE are dispensable for immunity to the forms of candidiasis evaluated, and illustrate a surprisingly limited specificity of the IL-17 family of cytokines with respect to systemic, oral and cutaneous Candida infections. PMID:25849644

  2. Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

    Rençber, Seda; Karavana, Sinem Yaprak; Yılmaz, Fethiye Ferda; Eraç, Bayri; Nenni, Merve; Özbal, Seda; Pekçetin, Çetin; Gurer-Orhan, Hande; Hoşgör-Limoncu, Mine; Güneri, Pelin; Ertan, Gökhan

    2016-01-01

    This study aimed to develop a suitable buccal mucoadhesive nanoparticle (NP) formulation containing fluconazole for the local treatment of oral candidiasis. The suitability of the prepared formulations was assessed by means of particle size (PS), polydispersity index, and zeta potential measurements, morphology analysis, mucoadhesion studies, drug entrapment efficiency (EE), in vitro drug release, and stability studies. Based on the optimum NP formulation, ex vivo drug diffusion and in vitro cytotoxicity studies were performed. Besides, evaluation of the antifungal effect of the optimum formulation was evaluated using agar diffusion method, fungicidal activity-related in vitro release study, and time-dependent fungicidal activity. The effect of the optimum NP formulation on the healing of oral candidiasis was investigated in an animal model, which was employed for the first time in this study. The zeta potential, mucoadhesion, and in vitro drug release studies of various NP formulations revealed that chitosan-coated NP formulation containing EUDRAGIT® RS 2.5% had superior properties than other formulations. Concerning the stability study of the selected formulation, the formulation was found to be stable for 6 months. During the ex vivo drug diffusion study, no drug was found in receptor phase, and this is an indication of local effect. The in vitro antifungal activity studies showed the in vitro efficacy of the NP against Candida albicans for an extended period. Also, the formulation had no cytotoxic effect at the tested concentration. For the in vivo experiments, infected rabbits were successfully treated with local administration of the optimum NP formulation once a day. This study has shown that the mucoadhesive NP formulation containing fluconazole is a promising candidate with once-a-day application for the local treatment of oral candidiasis. PMID:27358561

  3. The efficacy and safety of clotrimazole vaginal tablet vs. oral fluconazole in treating severe vulvovaginal candidiasis.

    Zhou, Xiaofang; Li, Ting; Fan, Shangrong; Zhu, Yuxia; Liu, Xiaoping; Guo, Xuedong; Liang, Yiheng

    2016-07-01

    To compare the efficacy and safety of two doses of clotrimazole vaginal tablet 500 mg with two doses of oral fluconazole 150 mg in treating severe vulvovaginal candidiasis (SVVC), 240 consecutive patients with SVVC were studied at the Department of Obstetrics and Gynaecology of Peking University Shenzhen Hospital between June 2014, and September 2015. Patients were randomly assigned in a 1 : 1 ratio to receive treatment with either two doses of clotrimazole vaginal tablet or two doses of oral fluconazole. The clinical cure rates in the clotrimazole group and the fluconazole group at days 7-14 follow-up were 88.7% (102/115) and 89.1% (98/110) respectively; the clinical cure rates at days 30-35 in the two groups were 71.9% (82/114) and 78.0% (85/109) respectively. The mycological cure rates at days 7-14 follow-up in the two groups were 78.3% (90/115) and 73.6% (81/110) respectively. The mycological cure rates of the patients at days 30-35 in the two groups were 54.4% (62/114) and 56.0% (61/109) respectively (P > 0.05). The adverse events of clotrimazole were mainly local. This study demonstrated that two doses of clotrimazole vaginal tablet 500 mg were as effective as two doses of oral fluconazole 150 mg in the treatment of patients with SVVC and could be an appropriate treatment for this disorder. PMID:27073145

  4. [Inhibitory activity of vaginal Lactobacillus bacteria on yeasts causing vulvovaginal candidiasis].

    Strus, Magdalena; Brzychczy-Włoch, Monika; Kucharska, Agnieszka; Gosiewski, Tomasz; Heczko, Piotr B

    2005-01-01

    Growing frequency of therapeutical failures of vulvovaginal candidiasis, resulting from resistance of certain species of Candida to imidazole agents, raises interest in the use of probiotics from Lactobacillus genera as prophylaxis. Unfortunately, little is known about inhibitory mechanisms of Lactobacillus on Candida. The aim of this study was to compare the activity of selected Lactobacillus species, representing the physiological vaginal flora, against Candida as well as investigation whether their inhibitory activity against Candida is related strictly to hydrogen peroxide and lactic acid production. 125 strains from vaginal smears of healthy women were classified by making use of phenotypic and genotypic methods. The majority of strains belonged to L. acidophilus: L. acidophilus sensu stricto, L. crispatus, L. gasseri and L. johnsonii as well as L. fermentum and L. plantarum species. Culture supernatants of selected 25 strains representing the isolated species were examined for their inhibitory activity against the growth of Candida albicans and C. glabrata. The results showed that the strongest and the fastest activity against C. albicans was demonstrated by L. delbrueckii strains, producing the largest quantities of hydrogen peroxide. On the other hand, extended activity, demonstrable after 24 hours, was shown by non-H2O2 producing L. plantarum supernatants. Growth of C. glabrata was not inhibited by any of the examined strains of Lactobacillus. Comparison of activity of live active cultures of Lactobacillus strains and their mixtures with this of pure H2O2 and lactic acid has shown that pure chemical compounds were less active than the cultures. This suggests that mixtures of Lactobacillus strains are in cooperation with each other using many different metabolites. PMID:16130291

  5. Preparation and characterisation of fluconazole vaginal films for the treatment of vaginal Candidiasis

    L Kumar

    2013-01-01

    Full Text Available Objective of the present study was to develop and evaluate vaginal films with essential in vitro studies. Films were developed using hydroxypropyl methylcellulose as a polymer and formulations were coded. The developed films were evaluated with Fourier transform infrared spectroscopy, drug content, viscosity, surface pH, thickness, mechanical characterisation and in vitro drug release study. Fourier transform infrared spectroscopy results confirmed that there is no chemical interaction between drug and stabilisers/excipients. The batch variation was not more than 5% for average thickness and weight of the films. The drug content for the prepared formulation was in the range of 72.32±0.18% to 94.48±0.54%. Viscosity of the formulations increased with the increase in concentration of polymer. Mechanical characterisation revealed that tensile strength and percentage elongation of the films improved as there is increase in degree of substitution of the polymer, but the values of modulus decreased which confirmed that all the prepared films are soft in nature. The in vitro study indicated that 1 and 2% concentrations of polymer are the least concentrations to control the release of drug whereas the 4% concentration of polymer is a good and more effective concentration to control the release. Only one prepared formulation released the drug by following anomalous transport whereas other film formulations released the fluconazole by following Fickian diffusion mechanism. Prepared vaginal films may be an important alternative for the treatment of vaginal candidiasis, because these prepared films suggest the benefits of controlled release of fluconazole at the site of absorption.

  6. Spectrum and risk factors for invasive candidiasis and non-Candida fungal infections after liver transplantation

    SHI Shao-hua; LU An-wei; SHEN Yan; JIA Chang-ku; WANG Wei-lin; XIE Hai-yang; ZHANG Min; LIANG Ting-bo; ZHENG Shu-sen

    2008-01-01

    Background Invasive fungal infections are an important cause of posttransplant mortality in solid-organ recipients.The current trend is that the incidence of invasive candidiasis decreases significantly and invasive aspergillosis occurs later in the liver posttransplant recipients.The understanding of epidemiology and its evolving trends in the particular locality is beneficial to prophylactic and empiric treatment for transplant recipients.Methods A retrospective analysis was made of recorded data on the epidemiology,risk factors,and mortality of jnvasive fungal infections in 352 liver transplant recipients.Results Forty-two(11.9%)patients suffered from Invasive fungal infection.Candida species infections(53.3%)were the most common,followed by Aspergillus species(40.0%).There were 21 patients with a superficial fungal infection.The median time to onset of first invasive fungal infection was 13 days,first invasive Candida infection 9 days,and first invasive Aspergillus infection 21 days.Fifteen deaths were related to invasive fungal infection,10 to Aspergillus infection,and 5 to Candida infection.Invasive Candida species infections were associated with encephalopathy(P=0.009)and postoperative bacterial infection(P=0.0003)as demonstrated by multivariate analysis.Three independent risk factors of invasive Aspergillus infection were posttransplant laparotomy(P=0.004),renal dysfunction(P=0.005)and hemodialysis (P=0.001).Conclusions The leading etiologic species of invasive fungal infections are Candida and Aspergillus,which frequently occur in the first posttransplant month.EncephalOpathy and postoperative bacterial infection predispose to invasive Candida infection.POsttransDlant laparotomy and poor perioperative clinical status contribute to invasive Aspergillus infection.More studies are needed to determine the effect of prophylactic antifungal therapy in high risk Patients.

  7. Local Th1/Th2 Cytokine Expression in Experimental Murine Vaginal Candidiasis

    Weixiang OUYANG; Shanjuan CHEN; Zhixiang LIU; Yan WU; Jiawen LI

    2008-01-01

    In order to investigate the expression of Th1 and Th2 cytokines in the vaginal candidiasis caused by Candida, the fungal vaginitis model was established in female ICR mice by intravaginal inoculation of suspension of C. albicans after the animals were pretreated with estradiol. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of IL-2, IL-4, IL-10 and TGF-β1 in the vagina in the mice of different groups at different time points after the beginning of the experiment. The average expression level of IL-2 mRNA in group D (estrogen-treated mice) was significantly higher than that in groups H (estrogen-untreated mice) and I (control group) on the day 2. The average expression level of IL-4 mRNA in group D was significantly higher than that in groups I and H on the day 5. The average expression level of IL-10 mRNA in group D was significantly higher than that in groups H and I from day 7 to 11. The average expression level of TGF-β1 mRNA in group D was significantly higher than that in groups H and I at all time points. It was concludes that the high-level expression of IL-2 mRNA during early infection was associated with clearance of mucosal C. albicans, and the high-level expression of IL-10 mRNA during late stage of the infection was related to susceptibility to infection. TGF-β1 may play a predominant role when the virtual absence of changes in other Th-type cytokines during infection.

  8. Vaginal nystatin versus oral fluconazole for the treatment for recurrent vulvovaginal candidiasis.

    Fan, Shangrong; Liu, Xiaoping; Wu, Cong; Xu, Lixuan; Li, Jianling

    2015-02-01

    Recurrent vulvovaginal candidiasis (RVVC) is a common condition that can physically and psychologically impact patients. We compared the efficacy and safety of vaginal nystatin suppositories for 14 days each month versus standard oral fluconazole regimens for the treatment for RVVC. Patients (n = 293) were enrolled in the study from April 2010 to September 2013. After the initial therapy, the mycological cure rates were 78.3% (119/152) and 73.8% (104/141) in the nystatin group and fluconazole group, respectively (95% CI, 0.749-2.197, p > 0.05). The mycological cure rates at the end of maintenance therapy were 80.7% (96/119) and 72.7% (72/99) in the two groups, respectively (95% CI, 0.954-3.293, p > 0.05).The mycological cure rates at the end without treatment for 6 months were 81.25% (78/96) and 82.19% (60/73) in the two groups, respectively (95% CI, 0.427-2.066, p > 0.05). The mycological cure rates of RVVC caused by C. albicans were 84.0% (89/106) and 81.8% (99/121) in the two groups, respectively. The mycological cure rates of RVVC caused by C. glabrata were 64.3% (27/42) and 12.5% (2/16) in the two groups, respectively. The initial and 6-month maintenance therapy were successful in five of the nine patients in the nystatin group with RVVC caused by fluconazole-resistant Candida, whereas in the fluconazole group, initial therapy failed in all patients with RVVC caused by fluconazole-resistant Candida (n = 7). We conclude that both fluconazole and nystatin therapies are effective in treating RVVC. Nystatin may also be effective for the treatment for RVVC caused by C. glabrata or fluconazole-resistant Candida. PMID:25416649

  9. Mucosal candidiasis elicits NF-κB activation, proinflammatory gene expression and localized neutrophilia in zebrafish.

    Gratacap, Remi L; Rawls, John F; Wheeler, Robert T

    2013-09-01

    The epithelium performs a balancing act at the interface between an animal and its environment to enable both pathogen killing and tolerance of commensal microorganisms. Candida albicans is a clinically important human commensal that colonizes all human mucosal surfaces, yet is largely prevented from causing mucosal infections in immunocompetent individuals. Despite the importance of understanding host-pathogen interactions at the epithelium, no immunocompetent vertebrate model has been used to visualize these dynamics non-invasively. Here we demonstrate important similarities between swimbladder candidiasis in the transparent zebrafish and mucosal infection at the mammalian epithelium. Specifically, in the zebrafish swimmbladder infection model, we show dimorphic fungal growth, both localized and tissue-wide epithelial NF-κB activation, induction of NF-κB -dependent proinflammatory genes, and strong neutrophilia. Consistent with density-dependence models of host response based primarily on tissue culture experiments, we show that only high-level infection provokes widespread activation of NF-κB in epithelial cells and induction of proinflammatory genes. Similar to what has been found using in vitro mammalian models, we find that epithelial NF-κB activation can occur at a distance from the immediate site of contact with epithelial cells. Taking advantage of the ability to non-invasively image infection and host signaling at high resolution, we also report that epithelial NF-κB activation is diminished when phagocytes control the infection. This is the first system to model host response to mucosal infection in the juvenile zebrafish, and offers unique opportunities to investigate the tripartite interactions of C. albicans, epithelium and immune cells in an intact host. PMID:23720235

  10. Patient preferences and treatment safety for uncomplicated vulvovaginal candidiasis in primary health care

    Hernández Susana

    2011-01-01

    Full Text Available Abstract Background Vaginitis is a common complaint in primary care. In uncomplicated candidal vaginitis, there are no differences in effectiveness between oral or vaginal treatment. Some studies describe that the preferred treatment is the oral one, but a Cochrane's review points out inconsistencies associated with the report of the preferred way that limit the use of such data. Risk factors associated with recurrent vulvovaginal candidiasis still remain controversial. Methods/Design This work describes a protocol of a multicentric prospective observational study with one year follow up, to describe the women's reasons and preferences to choose the way of administration (oral vs topical in the treatment of not complicated candidal vaginitis. The number of women required is 765, they are chosen by consecutive sampling. All of whom are aged 16 and over with vaginal discharge and/or vaginal pruritus, diagnosed with not complicated vulvovaginitis in Primary Care in Madrid. The main outcome variable is the preferences of the patients in treatment choice; secondary outcome variables are time to symptoms relief and adverse reactions and the frequency of recurrent vulvovaginitis and the risk factors. In the statistical analysis, for the main objective will be descriptive for each of the variables, bivariant analysis and multivariate analysis (logistic regression.. The dependent variable being the type of treatment chosen (oral or topical and the independent, the variables that after bivariant analysis, have been associated to the treatment preference. Discussion Clinical decisions, recommendations, and practice guidelines must not only attend to the best available evidence, but also to the values and preferences of the informed patient.

  11. Pancreatic candidiasis that mimics a malignant pancreatic cystic tumor on magnetic resonance imaging: A case report in an immunocompetent patient

    Seong, Min Jung; Kang, Tae Wook; Ha, Sang Yun [Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2015-12-15

    Candida is a commensal organism that is frequently found in the human gastrointestinal tract. It is the most common organism that causes pancreatic fungal infections. However, magnetic resonance imaging findings of Candida infection in the pancreas have not been described. We report imaging findings of pancreatic candidiasis in a patient in immunocompetent condition. It presented as a multi-septated cystic mass with a peripheral solid component in the background of pancreatitis and restricted diffusion on diffusion-weighted image that mimicked a malignant pancreatic cystic tumor.

  12. Recurrent candidiasis and early-onset gastric cancer in a patient with a genetically defined partial MYD88 defect.

    Vogelaar, Ingrid P; Ligtenberg, Marjolijn J L; van der Post, Rachel S; de Voer, Richarda M; Kets, C Marleen; Jansen, Trees J G; Jacobs, Liesbeth; Schreibelt, Gerty; de Vries, I Jolanda M; Netea, Mihai G; Hoogerbrugge, Nicoline

    2016-04-01

    Gastric cancer is caused by both genetic and environmental factors. A woman who suffered from recurrent candidiasis throughout her life developed diffuse-type gastric cancer at the age of 23 years. Using whole-exome sequencing we identified a germline homozygous missense variant in MYD88. Immunological assays on peripheral blood mononuclear cells revealed an impaired immune response upon stimulation with Candida albicans, characterized by a defective production of the cytokine interleukin-17. Our data suggest that a genetic defect in MYD88 results in an impaired immune response and may increase gastric cancer risk. PMID:26700889

  13. Protocol for a randomised controlled trial of treatment of asymptomatic candidiasis for the prevention of preterm birth [ACTRN12610000607077

    Rickard Kristen R

    2011-03-01

    Full Text Available Abstract Background Prevention of preterm birth remains one of the most important challenges in maternity care. We propose a randomised trial with: a simple Candida testing protocol that can be easily incorporated into usual antenatal care; a simple, well accepted, treatment intervention; and assessment of outcomes from validated, routinely-collected, computerised databases. Methods/Design Using a prospective, randomised, open-label, blinded-endpoint (PROBE study design, we aim to evaluate whether treating women with asymptomatic vaginal candidiasis early in pregnancy is effective in preventing spontaneous preterm birth. Pregnant women presenting for antenatal care The study protocol draws on the usual antenatal care schedule, has been pilot-tested and the intervention involves only a minor modification of current practice. Women who agree to participate will self-collect a vaginal swab and those who are culture positive for Candida will be randomised (central, telephone to open-label treatment or usual care (screening result is not revealed, no treatment, routine antenatal care. Outcomes will be obtained from population databases. A sample size of 3,208 women with Candida colonisation (1,604 per arm is required to detect a 40% reduction in the spontaneous preterm birth rate among women with asymptomatic candidiasis from 5.0% in the control group to 3.0% in women treated with clotrimazole (significance 0.05, power 0.8. Analyses will be by intention to treat. Discussion For our hypothesis, a placebo-controlled trial had major disadvantages: a placebo arm would not represent current clinical practice; knowledge of vaginal colonisation with Candida may change participants' behaviour; and a placebo with an alcohol preservative may have an independent affect on vaginal flora. These disadvantages can be overcome by the PROBE study design. This trial will provide definitive evidence on whether screening for and treating asymptomatic candidiasis in

  14. A STAT1-gain-of-function mutation causing Th17 deficiency with chronic mucocutaneous candidiasis, psoriasiform hyperkeratosis and dermatophytosis.

    Nielsen, Jakob; Kofod-Olsen, Emil; Spaun, Eva; Larsen, Carsten S; Christiansen, Mette; Mogensen, Trine Hyrup

    2015-01-01

    During recent years, inborn errors of human IL-17 immunity have been demonstrated to underlie primary immunodeficiencies with chronic mucocutaneous candidiasis (CMC). Various defects in receptors responsible for sensing of Candida albicans or downstream signalling to IL-17 may lead to susceptibility to Candida infection. While CMC is common in patients with profound T cell immunodeficiencies, CMC is also recognised as part of other immunodeficiencies in syndromic CMC, or as relatively isolated CMC disease. We describe a 40-year-old woman with a clinical picture involving cutaneous bacterial abscesses, chronic oral candidiasis and extensive dermatophytic infection of the feet. By whole exome sequencing, we identified a STAT1-gain-of-function mutation. Moreover, the patient's peripheral blood mononuclear cells displayed severely impaired Th17 responses. The patient was treated with antifungals and prophylactic antibiotics, which led to resolution of the infection. We discuss the current knowledge within the field of Th17 deficiency and the pathogenesis and treatment of CMC. PMID:26494717

  15. Correlation between in vitro and in vivo antifungal activities in experimental fluconazole-resistant oropharyngeal and esophageal candidiasis.

    Walsh, T J; Gonzalez, C E; Piscitelli, S; Bacher, J D; Peter, J; Torres, R; Shetti, D; Katsov, V; Kligys, K; Lyman, C A

    2000-06-01

    Oropharyngeal and esophageal candidiasis (OPEC) is a frequent opportunistic mycosis in immunocompromised patients. Azole-resistant OPEC is a refractory form of this infection occurring particularly in human immunodeficiency virus (HIV)-infected patients. The procedures developed by the Antifungal Subcommittee of the National Committee for Clinical Laboratory Standards (NCCLS) are an important advance in standardization of in vitro antifungal susceptibility methodology. In order to further understand the relationship between NCCLS methodology and antifungal therapeutic response, we studied the potential correlation between in vitro susceptibility to fluconazole and in vivo response in a rabbit model of fluconazole-resistant OPEC. MICs of fluconazole were determined by NCCLS methods. Three fluconazole-susceptible (FS) (MIC, /=64 microgram/ml) isolates of Candida albicans from prospectively monitored HIV-infected children with OPEC were studied. FR isolates were recovered from children with severe OPEC refractory to fluconazole, and FS isolates were recovered from those with mucosal candidiasis responsive to fluconazole. Fluconazole at 2 mg/kg of body weight/day was administered to infected animals for 7 days. The concentrations of fluconazole in plasma were maintained above the MICs for FS isolates throughout the dosing interval. Fluconazole concentrations in the esophagus were greater than or equal to those in plasma. Rabbits infected with FS isolates and treated with fluconazole had significant reductions in oral mucosal quantitative cultures (P OPEC due to C. albicans. PMID:10835005

  16. Evaluation of efficacy and safety of itraconazole oral solution for the treatment of oropharyngeal candidiasis in AIDS patients

    Queiroz-Telles Flávio

    2001-01-01

    Full Text Available This study was a non-comparative multicenter clinical trial to evaluate the efficacy and tolerability of itraconazole oral solution 200 mg/day (100 mg twice a day in the fasting state for the treatment of oropharyngeal candidiasis in AIDS patients. We included 50 patients who were treated and followed for up to 3 weeks after ending therapy in the analysis. Mycological cures at the end of therapy occurred in 20/50 patients (40%, but colonization by Candida sp. was recorded in 42/50 (84% by the end of follow-up. A high rate of clinical response was observed in 46/50 (92%, and the response was sustained for up to 21 days after stopping therapy in 24/46 patients (52%. Clinical relapses were documented among 22 patients, but all causative fungal organisms associated with a relapse were susceptible to itraconazole. There were many patients with persistence or recurrence of Candida, but without mucositis. Relapse of Candida mucositis was significantly related to low levels of CD4 lymphocytes exhibited by symptomatic patients. The drug was well tolerated by all but 1 patient. We conclude that itraconazole oral solution (100 mg bid for 7-14 days is a well tolerated and effective treatment for suppressing the symptoms of oropharyngeal candidiasis in AIDS patients. Patients with severe immunosuppression may relapse and require frequent cycles of treatment or longterm suppressive therapy.

  17. Epigastric Distress Caused by Esophageal Candidiasis in 2 Patients Who Received Sorafenib Plus Radiotherapy for Hepatocellular Carcinoma: Case Report.

    Chen, Kuo-Hsin; Weng, Meng-Tzu; Chou, Yueh-Hung; Lu, Yueh-Feng; Hsieh, Chen-Hsi

    2016-03-01

    Sorafenib followed by fractionated radiotherapy (RT) has been shown to decrease the phagocytic and candidacidal activities of antifungal agents due to radiosensitization. Moreover, sorafenib has been shown to suppress the immune system, thereby increasing the risk for candida colonization and infection. In this study, we present the 2 hepatocellular carcinoma (HCC) patients suffered from epigastric distress caused by esophageal candidiasis who received sorafenib plus RT.Two patients who had received sorafenib and RT for HCC with bone metastasis presented with hiccups, gastric ulcer, epigastric distress, anorexia, heart burn, and fatigue. Empiric antiemetic agents, antacids, and pain killers were ineffective at relieving symptoms. Panendoscopy revealed diffuse white lesions in the esophagus. Candida esophagitis was suspected. Results of periodic acid-Schiff staining were diagnostic of candidiasis. Oral fluconazole (150 mg) twice daily and proton-pump inhibitors were prescribed. At 2-weak follow-up, esophagitis had resolved and both patients were free of gastrointestinal symptoms.Physicians should be aware that sorafenib combined with RT may induce an immunosuppressive state in patients with HCC, thereby increasing their risk of developing esophagitis due to candida species. PMID:26986168

  18. "PCR- Detection of Candida albicans in Blood Using a New Primer Pair to Diagnosis of Systemic Candidiasis"

    SH Mirhendi

    2003-07-01

    Full Text Available The opportunistic pathogen C.albicans is able to cause disseminated infections in immunocompromised patients. Microbiological methods for the diagnosis of invasive candidiasis have many problems including low sensitivity, requirement to invasive clinical sampling such as biopsies or multiple blood cultures and need to expertise laboratory stuff. Since PCR has proven to be a powerful tool in the early diagnosis of several infectious diseases, we applied this approach as a rapid and sensitive method in detection of C.albicans cells in blood samples, for establishment a clinically useful method in diagnosing systemic candidiasis. DNA were extracted from blood samples seeded by serially diluted C.albicans cells, by omitting WBC and RBC followed by enzymatic breaking of fungal cell wall and phenol – chlorophorm extraction and alcohol precipitation of DNA. A new primer pair was designed for PCR-amplification of a part of ribosomal RNA gene. The primer set was able to amplify all medically important Candida species. When PCR was performed for detection of purified DNA, the sensitivity of the method was about 1 picogram fungal DNA, whereas the sensitivity for detection of C.albicans blastospores inoculated in blood was as few as 10 cell per 0.1 ml of blood. This method could be sensitive and useful for early and rapid diagnosis of systemic Candida infections and to simultaneous detection and speciation of Candida species by PCR-RFLP method.

  19. Does Scientific Evidence for the Use of Natural Products in the Treatment of Oral Candidiasis Exist? A Systematic Review

    Gabriela Lacet Silva Ferreira

    2015-01-01

    Full Text Available In view of the limitations of antifungal agents used in the treatment of oral candidiasis and the wide variety of natural products that have been studied as treatment of this disease, this systematic literature review proposed to evaluate whether scientific evidence attesting to the efficacy of natural products in the treatment of this disease exists. A systematic search in PubMed, MEDLINE, SciELO, Lilacs, and Cochrane Library databases was accomplished using the associations among the keywords Candida albicans, phytotherapy, biological products, denture stomatitis, and oral candidiasis in both English and Portuguese. Four independent observers evaluated the methodological quality of the resulting articles. Three studies were included for detailed analysis and evaluated according to the analysis protocol based on the CONSORT (Consolidated Standards of Reporting Trials 2010 statement. The tested products were different in all studies. Two studies mentioned random samples, but no study described the sample allocation. No study mentioned sample calculations, a prior pilot study, or examiner calibration, and only one trial reported sample losses. Differences between the tested products and the methodological designs among these studies did not allow the existence of scientific evidence related to the effectiveness of these products for the proposed subjects to be confirmed.

  20. In-Vitro Cytokine Induction and Neutrophil Respiratory Burst Activity by Candida Isolates from HIV Seropositive Patients with Oropharyngeal Candidiasis.

    Mayuri Rani

    2014-06-01

    Full Text Available The importance of host defense against candidiasis and role of cell mediated immunity in host defense has been the subject of many studies. Increased expression of virulence factors in candida isolates from human immunodeficiency virus (HIV seropositive patients with oropharyngeal candidiasis (OPC has also been reported. We conducted an experiment to study the difference in T helper type 2 (Th2 and T helper type 1 (Th1 responses and neutrophil respiratory burst responses, of naive peripheral blood mononuclear cells (PBMC’s and neutrophils respectively, by using Candida isolates from study and control groups as stimulus. Thirty HIV seropositive patients with clinical evidence of OPC and thirty healthy volunteers were studied. IL-10 release was uniformly low at all concentrations of Candida albicans antigen isolated from healthy controls as compared to when C. albicans isolated from HIV seropositive patients was used as antigenic stimulus. Mean IFN-ᵧ concentration was highest when C.albicans, isolated from healthy carriers was used as antigenic stimulus for PBMCs. The observations highlight a significant IL-10 dominance, which may be an indicator of the fine tuning of host immune system towards a chronic progressive disease condition,indicating the pathogenic potential of select Candida strains.

  1. Low-dose ketoconazole-fluconazole combination versus fluconazole in single doses for the treatment of vaginal candidiasis

    Jan Susilo

    2011-08-01

    Full Text Available Background: Vaginal candidiasis (VC is one of the most common fungal diseases. Candida albicans is the most common causative fungus and has been isolated from more than 80% of specimens obtained from women with VC. Ketoconazole is the first orally active antifungal, the dosage for VC is 200 mg twice daily for 5 days. Fluconazole is the newer oral antifungal, its dosage for VC is a single oral dose of 150 mg. Since fluconazole 150 mg is considerably expensive, a single dose of 100 mg ketoconazole and 40 mg fluconazole in combination has been tested for the treatment of VC. The results showed that from 11 women with confirmed VC, 1-2 weeks after drug administration, the mycological culture was negative in 8 women, positive in 1 woman, and 2 woman lost to follow-up. This promising result led to the present study with the objective to confirm the efficacy and safety of the above combination in a formal clinical trial.Methods: A total of 165 female patients, aged 18 years or older, with the diagnosis of VC from clinical symptoms (pruritus or burning or excessive discharge and positive microscopic smear (pseudohyphae and/or yeast cells were randomized to receive a single dose of either keto-fluco combination (n = 85 or fluconazole (n = 80, and returnedfor follow-up visit on day 8.Results: Among these patients, 39 patients had negative baseline culture, leaving 126 patients eligible for efficacy evaluation. The mycological eradication in the keto-fluco group was 74.5% (41 patients from a total of 55 patients with available mycological culture, while that in the fluconazole group was 70.2% (40 patients from 57 patients with available culture and this difference was not significant. The clinical favorable response (clinical cure and clinical improvement in the keto-fluco arm (n = 60 was 98.3%, while that in the fluconazole group (n = 66 was 100%. Adverse events were found in 5 patients, 3 patients in the keto-fluco group (3/85 = 3.5% and 2

  2. Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds.

    Kandimalla, Raghuram; Kalita, Sanjeeb; Choudhury, Bhaswati; Dash, Suvakanta; Kalita, Kasturi; Kotoky, Jibon

    2016-01-01

    Poor wound healing is one of the major complication of diabetic patients which arises due to different factors like hyperglycemia, oxidative stress, vascular insufficiency and microbial infections. Candidiasis of diabetic wounds is a difficult to treat condition and potentially can lead to organ amputation. There are a few number of medications available in market to treat this chronic condition; which demands for alternative treatment options. In traditional system of medicine like Ayurveda, essential oil extracted from leaves of Cymbopogon nardus L. (Poaceae) has been using for the treatment of microbial infections, inflammation and pain. In this regard, we have evaluated anti-Candida and anti-inflammatory activity mediated wound healing property of C. nardus essential oil (EO-CN) on candidiasis of diabetic wounds. EO-CN was obtained through hydro-distillation and subjected to Gas chromatography-mass spectroscopy (GC-MS) analysis for chemical profiling. Anti-Candida activity of EO-CN was tested against Candida albicans, C. glabrata and C. tropicalis by in vitro zone of inhibition and minimum inhibitory concentration (MIC) assays. Anti-candidiasis ability of EO-CN was evaluated on C. albicans infected diabetic wounds of mice through measuring candida load on the 7th, 14th, and 21st day of treatment. Further progression in wound healing was confirmed by measuring the inflammatory marker levels and histopathology of wounded tissues on last day of EO-CN treatment. A total of 95 compounds were identified through GC-MS analysis, with major compounds like citral, 2,6-octadienal-, 3,7-dimethyl-, geranyl acetate, citronellal, geraniol, and citronellol. In vitro test results demonstrated strong anti-Candida activity of EO-CN with a MIC value of 25 μg/ml against C. albicans, 50 μg/ml against C. glabrata and C. tropicalis. EO-CN treatment resulted in significant reduction of candida load on diabetic wounds. Acceleration in wound healing was indicated by declined levels of

  3. Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds

    Kandimalla, Raghuram; Kalita, Sanjeeb; Choudhury, Bhaswati; Dash, Suvakanta; Kalita, Kasturi; Kotoky, Jibon

    2016-01-01

    Poor wound healing is one of the major complication of diabetic patients which arises due to different factors like hyperglycemia, oxidative stress, vascular insufficiency and microbial infections. Candidiasis of diabetic wounds is a difficult to treat condition and potentially can lead to organ amputation. There are a few number of medications available in market to treat this chronic condition; which demands for alternative treatment options. In traditional system of medicine like Ayurveda, essential oil extracted from leaves of Cymbopogon nardus L. (Poaceae) has been using for the treatment of microbial infections, inflammation and pain. In this regard, we have evaluated anti-Candida and anti-inflammatory activity mediated wound healing property of C. nardus essential oil (EO-CN) on candidiasis of diabetic wounds. EO-CN was obtained through hydro-distillation and subjected to Gas chromatography–mass spectroscopy (GC–MS) analysis for chemical profiling. Anti-Candida activity of EO-CN was tested against Candida albicans, C. glabrata and C. tropicalis by in vitro zone of inhibition and minimum inhibitory concentration (MIC) assays. Anti-candidiasis ability of EO-CN was evaluated on C. albicans infected diabetic wounds of mice through measuring candida load on the 7th, 14th, and 21st day of treatment. Further progression in wound healing was confirmed by measuring the inflammatory marker levels and histopathology of wounded tissues on last day of EO-CN treatment. A total of 95 compounds were identified through GC–MS analysis, with major compounds like citral, 2,6-octadienal-, 3,7-dimethyl-, geranyl acetate, citronellal, geraniol, and citronellol. In vitro test results demonstrated strong anti-Candida activity of EO-CN with a MIC value of 25 μg/ml against C. albicans, 50 μg/ml against C. glabrata and C. tropicalis. EO-CN treatment resulted in significant reduction of candida load on diabetic wounds. Acceleration in wound healing was indicated by declined

  4. A Phase 2, Randomized, Double-Blind, Multicenter Trial To Evaluate the Safety and Efficacy of Three Dosing Regimens of Isavuconazole Compared with Fluconazole in Patients with Uncomplicated Esophageal Candidiasis

    Viljoen, J.; Azie, N.; Schmitt-Hoffmann, A.-H.; Ghannoum, M.

    2015-01-01

    Esophageal candidiasis is a frequent cause of morbidity in immunocompromised patients. Isavuconazole is a novel, broad-spectrum antifungal developed for the treatment of opportunistic fungal infections. This phase 2 trial compared the efficacy and safety of three oral dosing regimens of isavuconazole with an oral fluconazole regimen in the primary treatment of uncomplicated esophageal candidiasis. The isavuconazole regimens were as follows: 200 mg on day 1 and then 50 mg once daily (arm A), 4...

  5. Pharmacodynamics of Caspofungin in a Murine Model of Systemic Candidiasis: Importance of Persistence of Caspofungin in Tissues to Understanding Drug Activity

    Louie, Arnold; Deziel, Mark; Liu, Weiguo; Drusano, Michael F.; Gumbo, Tawanda; Drusano, George L.

    2005-01-01

    Pharmacokinetic and pharmacodynamic studies were conducted in a murine model of systemic candidiasis to determine the pharmacodynamic parameter linked with caspofungin efficacy. Additional studies defined the importance of persistent tissue drug concentrations to treatment outcome. The pharmacokinetics of caspofungin were determined in the serum and kidneys of infected mice over 96 h. Population pharmacokinetic analysis demonstrated a serum terminal half-life (t1/2) for caspofungin of 20.2 h ...

  6. Sexually transmitted infections, bacterial vaginosis, and candidiasis in women of reproductive age in rural Northeast Brazil: a population-based study

    Fabíola Araújo Oliveira

    2007-09-01

    Full Text Available Population-based data on sexually transmitted infections (STI, bacterial vaginosis (BV, and candidiasis reflect the epidemiological situation more accurately than studies performed in specific populations, but such data are scarce. To determine the prevalence of STI, BV, and candidiasis among women of reproductive age from a resource-poor community in Northeast Brazil, a population-based cross sectional study was undertaken. All women from seven hamlets and the centre of Pacoti municipality in the state of Ceará, aged 12 to 49 years, were invited to participate. The women were asked about socio-demographic characteristics and genital symptoms, and thereafter examined gynaecologically. Laboratory testing included polymerase chain reaction (PCR for human papillomavirus (HPV, ligase chain reaction (LCR for Chlamydia trachomatis and Neisseria gonorrhoeae, ELISA for human immunodeficiency virus (HIV, venereal disease research laboratory (VDRL and fluorescent treponema antibody absorption test (FTA-ABS for syphilis, and analysis of wet mounts, gram stains and Pap smears for trichomoniasis, candidiasis, and BV. Only women who had initiated sexual life were included in the analysis (n = 592. The prevalences of STI were: HPV 11.7% (95% confidence interval: 9.3-14.7, chlamydia 4.5% (3.0-6.6, trichomoniasis 4.1% (2.7-6.1, gonorrhoea 1.2% (0.5-2.6, syphilis 0.2% (0.0-1.1, and HIV 0%. The prevalence of BV and candidiasis was 20% (16.9-23.6 and 12.5% (10.0-15.5, respectively. The most common gynaecological complaint was lower abdominal pain. STI are common in women in rural Brazil and represent an important health threat in view of the HIV pandemic.

  7. Oropharyngeal Candidiasis in HIV Infection: Analysis of Impaired Mucosal Immune Response to Candida albicans in Mice Expressing the HIV-1 Transgene

    Louis de Repentigny; Mathieu Goupil; Paul Jolicoeur

    2015-01-01

    IL-17-producing Th17 cells are of critical importance in host defense against oropharyngeal candidiasis (OPC). Speculation about defective Th17 responses to oral C. albicans infection in the context of HIV infection prompted an investigation of innate and adaptive immune responses to Candida albicans in transgenic mice expressing the genome of HIV-1 in immune cells and displaying an AIDS-like disease. Defective IL-17 and IL-22-dependent mucosal responses to C. albicans were found to determin...

  8. Anti-candidal activity of Astragalus verus in the in vitro and in vivo guinea pig models of cutaneous and systemic candidiasis

    Ali Mikaeili

    2012-10-01

    Full Text Available This study was design to evaluate the anti-candidal activity of Astragalus verus Olivier, Fabaceae (Av. The GC/MS analysis of essential oils of Av showed that aqueous extract contains thymol while hexadecanoic acid, 1,2-benzenedicarboxylic acid, diisooctyl ester and phytol were found as major components of methanol and acetone extracts. The aqueous extract showed anti-candidal activity in the concentration 320 mg/mL using disc diffusion method and its minimum inhibitory concentration (MIC was 160 mg/mL. To induce cutaneous candidiasis, the dorsum of immunocompromised guinea pigs was infected with Candida albicans and animals were divided into five groups (n=5 for each: NC, received a vehicle; PC, received topical ketoconazole 2% and three other groups which received topical 10, 20 and 40% aqueous extract of Av. On ninth day postinfection, skins were cultured and colony forming unite per gram (CFU/g skin was recorded. Systemic candidiasis was obtained by intravenous inoculation of C. albicans, 4000 CFU/g body weight. Here, animals have been divided into five groups like cutaneous candidiasis but their medications have been delivered in drinking water for ten days before induction of infection. On second day postinfection, all internal tissues were taken for determining CFU/g tissue. The aqueous extract (40% prevented heavy burden of C. albicans in tissues and skin in oral and topical application, respectively. The results indicate that Av represents a potential source of anti-candidal drug.

  9. Candidiasis and other oral mucosal lesions during and after interferon therapy for HCV-related chronic liver diseases

    Nagao Yumiko

    2012-11-01

    Full Text Available Abstract Background Oral lichen planus (OLP is seen frequently in patients with hepatitis C virus (HCV infection. The aim of this study was to evaluate the occurrence of oral candidiasis, other mucosal lesions, and xerostomia during interferon (IFN therapy for HCV infection. Methods Of 124 patients with HCV-infected liver diseases treated with IFN therapy in our hospital, 14 (mean age 56.00 ± 12.94 years who attended to receive administration of IFN once a week were identified and examined for Candida infection and other oral lesions and for the measurement of salivary flow. Serological assays also were carried out. Results Cultures of Candida from the tongue surfaces were positive in 7 (50.0% of the 14 patients with HCV infection at least once during IFN therapy. C. albicans was the most common species isolated. The incidence of Candida during treatment with IFN did not increase above that before treatment. Additional oral mucosal lesions were observed in 50.0% (7/14 of patients: OLP in three (21.4%, angular cheilitis in three (21.4% and recurrent aphthous stomatitis in one (7.1%. OLP occurred in one patient before treatment with IFN, in one during treatment and in one at the end of treatment. 85.7% of the oral lesions were treated with topical steroids. We compared the characteristics of the 7 patients in whom Candida was detected at least once during IFN therapy (group 1 and the 7 patients in whom Candida was not detected during IFN therapy (group 2. The prevalence of oral mucosal lesions (P=0.0075 and incidence of external use of steroids (P=0.0308 in group 1 were significantly higher than in group 2. The average body weight of group 1 decreased significantly compared to group 2 (P=0.0088. Salivary flow decreased in all subjects throughout the course of IFN treatment and returned at 6th months after the end of treatment. In group 1, the level of albumin at the beginning of the 6th month of IFN administration was lower than in group 2 (P=0

  10. Formulation and evaluation of a novel mucoadhesive drug delivery system to treat intestinal candidiasis in immunocompromised patients

    SuparnaDugal

    2011-12-01

    Full Text Available The limited solubility, and therefore bioavailability, of the antimycotic drug,itraconazole, used for the treatment of intestinal Candidiasis in immunocompromisedindividuals, has been well documented. Therapeutic regimen in these patients mayinclude daily administration of multiple doses of various drugs. Hence, improving theresidence time of therapeutic agents, would ensure a high continuous concentration inthe body and help decrease the dosing frequency. In our current study, we haveinvestigated a novel method of drug delivery, developed by utilizing the concept ofmucoadhesiveness, for the sustained release of the drug, itraconazole. Mucoadhesivebeads were prepared using two natural polymers, isabghula husk and alginate. Theminimum inhibitory concentration of itraconazole for Candida was found to be1.5milligram per millilitre. Accordingly, beads were prepared by ionic gelation methodusing calcium chloride as a crosslinking agent. Marked improvement in solubility of thedrug was noted after entrapment. Prepared beads were subjected to various evaluationsincluding particle size, swelling behaviour and mucoadhesivity. At pH 7.4, goodmucoadhesive property was exhibited up to 7 hours. Maximum swelling of beads wasobserved at 4hours in phosphate buffer after which the beads showed slight erosion.Fresh cock intestinal mucosa was used to assess the sustained release of itraconazolefrom the drug loaded beads and the reduction in candidal cells adhering to the mucosawas verified by the viable count technique. The results of our present study indicate thatmucoadhesive intestinal retentive isabghula- alginate beads could represent a promisingvehicle for drug delivery and help improve therapeutic efficacy and patient compliancein the future.

  11. Clotrimazole-cyclodextrin based approach for the management and treatment of Candidiasis - A formulation and chemistry-based evaluation.

    Mohammed, Noorullah Naqvi; Pandey, Pankaj; Khan, Nayaab S; Elokely, Khaled M; Liu, Haining; Doerksen, Robert J; Repka, Michael A

    2016-08-01

    Clotrimazole (CT) is a poorly soluble antifungal drug that is most commonly employed as a topical treatment in the management of vaginal candidiasis. The present work focuses on a formulation approach to enhance the solubility of CT using cyclodextrin (CD) complexation. A CT-CD complex was prepared by a co-precipitation method. Various characterization techniques such as differential scanning calorimetry, infrared (IR) and X-ray spectroscopy, scanning electron microscopy and nuclear magnetic resonance (NMR) spectroscopy were performed to evaluate the complex formation and to understand the interactions between CT and CD. Computational molecular modeling was performed using the Schrödinger suite and Gaussian 09 program to understand structural conformations of the complex. The phase solubility curve followed an AL-type curve, indicating formation of a 1:1 complex. Molecular docking studies supported the data obtained through NMR and IR studies. Enthalpy changes confirmed that complexation was an exothermic and enthalpically favorable phenomenon. The CT-CD complexes were formulated in a gel and evaluated for release and antifungal activity. The in vitro release studies performed using gels demonstrated a sustained release of CT from the CT-CD complex with the complex exhibiting improved release relative to the un-complexed CT. Complexed CT-CD exhibited better fungistatic activity toward different Candida species than un-complexed CT. PMID:25923135

  12. Efficacy of PLD-118, a novel inhibitor of candida isoleucyl-tRNA synthetase, against experimental oropharyngeal and esophageal candidiasis caused by fluconazole-resistant C. albicans.

    Petraitis, Vidmantas; Petraitiene, Ruta; Kelaher, Amy M; Sarafandi, Alia A; Sein, Tin; Mickiene, Diana; Bacher, John; Groll, Andreas H; Walsh, Thomas J

    2004-10-01

    PLD-118, formerly BAY 10-8888, is a synthetic antifungal derivative of the naturally occurring beta-amino acid cispentacin. We studied the activity of PLD-118 in escalating dosages against experimental oropharyngeal and esophageal candidiasis (OPEC) caused by fluconazole (FLC)-resistant Candida albicans in immunocompromised rabbits. Infection was established by fluconazole-resistant (MIC > 64 microg/ml) clinical isolates from patients with refractory esophageal candidiasis. Antifungal therapy was administered for 7 days. Study groups consisted of untreated controls; animals receiving PLD-118 at 4, 10, 25, or 50 mg/kg of body weight/day via intravenous (i.v.) twice daily (BID) injections; animals receiving FLC at 2 mg/kg/day via i.v. BID injections; and animals receiving desoxycholate amphotericin B (DAMB) i.v. at 0.5 mg/kg/day. PLD-118- and DAMB-treated animals showed a significant dosage-dependent clearance of C. albicans from the tongue, oropharynx, and esophagus in comparison to untreated controls (P candidiasis. PMID:15388459

  13. Improved detection of deeply invasive candidiasis with DNA aptamers specific binding to (1→3)-β-D-glucans from Candida albicans.

    Tang, X-L; Hua, Y; Guan, Q; Yuan, C-H

    2016-04-01

    Deeply invasive or disseminated candidiasis is a serious and often fatal complication that can occur frequently in immuno-compromised individuals. However, conventional diagnostic methods of Candida albicans display low sensitivity and lack of specificity; the development of rapid and accurate detection methods remains a high priority. Aptamers are single-strand DNA or RNA oligonucleotides that specifically bind to target molecules with high affinity. In this study, we sought to screen high-affinity DNA aptamers that specifically bound to (1→3)-β-D-glucans from cell wall of Candida albicans using a systematic evolution of ligands by exponential enrichment (SELEX) technique, and further evaluate the diagnostic potential for invasive or disseminated candidiasis with selected aptamers. (1→3)-β-D-glucans was purified from Candida albicans, and two single DNA aptamers (designated as AU1 and AD1) were selected. Analysis of dissociation constants and binding domains further revealed that these two selected single DNA aptamers (AU1 and AD1) showed high binding affinity (AD1: Kd = 79.76 nM, AD1: Kd = 103.7 nM) and did not bind to the same domain of (1→3)-β-D-glucans. Next, we further detected (1→3)-β-D-glucans in serum samples from different groups of patients with Candida albicans infection or simple bacterial infection by using a double-aptamer sandwich enzyme-linked oligonucleotide assay (ELONA). The results showed that the sensitivity and specificity of this aptamer-based sandwich ELONA were 92.31 % and 91.94 % respectively. Thus, our study suggests that AU1 and AD1 have potential application for the differentiate diagnosis of deeply invasive candidiasis and provide valuable clues for designing diagnostic agents for the identification of invasive fungal infection. PMID:26810058

  14. ALS1 and ALS3 gene expression and biofilm formation in Candida albicans isolated from vulvovaginal candidiasis

    Roudbarmohammadi, Shahla; Roudbary, Maryam; Bakhshi, Bita; Katiraee, Farzad; Mohammadi, Rasoul; Falahati, Mehraban

    2016-01-01

    Background: A cluster of genes are involved in the pathogenesis and adhesion of Candida albicans to mucosa and epithelial cells in the vagina, the important of which is agglutinin-like sequence (ALS) genes. As well as vaginitis is a significant health problem among women, the antifungal resistance of Candida species is continually increasing. This cross-sectional study investigates the expression of ALS1 and ALS3 genes and biofilm formation in C. albicans isolate isolated from vaginitis. Materials and Methods: Fifty-three recognized isolates of C. albicans were collected from women with recurrent vulvovaginal candidiasis in Iran, cultured on sabouraud dextrose agar, and then examined for gene expression. Total messenger RNA (mRNA) extracted from C. albicans isolates and complementary DNA synthesized using reverse transcriptase enzyme. Reverse transcription-polymerase chain reaction (RT-PCR) using specific primer was used to evaluate the expression of ALS1 and ALS3 through housekeeping (ACT1) genes. 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide assay was performed to assess adherence capacity and biofilm formation in the isolated. Results: Forty isolates (75.8%) expressed ALS1 and 41 isolates (77.7%) expressed ALS3 gene. Moreover, 39 isolates (74%) were positive for both ALS1 and ALS3 mRNA by the RT-PCR. Adherence capability in isolates with ALS1 or ALS3 genes expression was greater than the control group (with any gene expression), besides, it was significantly for the most in the isolates that expressed both ALS1 and ALS3 genes simultaneously. Conclusion: The results attained indicated that there is an association between the expression of ALS1 and ALS3 genes and fluconazole resistance in C. albicans. A considerable percent of the isolates expressing the ALS1 and ALS3 genes may have contributed to their adherence to vagina and biofilm formation. PMID:27376044

  15. Anticommensal Responses Are Associated with Regulatory T Cell Defect in Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy Patients.

    Hetemäki, Iivo; Jarva, Hanna; Kluger, Nicolas; Baldauf, Hanna-Mari; Laakso, Sini; Bratland, Eirik; Husebye, Eystein S; Kisand, Kai; Ranki, Annamari; Peterson, Pärt; Arstila, T Petteri

    2016-04-01

    Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is a monogenic autoimmune disease caused by mutations in the AIRE gene. Although mainly an endocrine disease, a substantial fraction of patients have gastrointestinal manifestations. In this study, we have examined the role of anticommensal responses and their regulation. APECED patients had increased levels of Abs against Saccharomyces cerevisiae (p < 0.0001) and against several species of commensal gut bacteria, but not against species predominantly associated with other locations. The anticommensal Ab levels did not correlate with gastrointestinal autoantibodies, neutralizing anti-IL-17 or -IL-22 Abs, or gastrointestinal symptoms, although scarcity of the available clinical data suggests that further study is required. However, the anti-S. cerevisiae Ab levels showed a significant inverse correlation with FOXP3 expression levels in regulatory T cells (Treg), previously shown to be dysfunctional in APECED. The correlation was strongest in the activated CD45RO(+) population (ρ = -0.706; p < 0.01). APECED patients also had decreased numbers of FOXP3(+) cells in gut biopsies. These results show that APECED patients develop early and sustained responses to gut microbial Ags in a pattern reminiscent of Crohn's disease. This abnormal immune recognition of gut commensals is linked to a systemic Treg defect, which is also reflected as a local decrease of gut-associated Treg. To our knowledge, these data are the first to show dysregulated responses to non-self commensal Ags in APECED and indicate that AIRE contributes to the regulation of gut homeostasis, at least indirectly. The data also raise the possibility of persistent microbial stimulation as a contributing factor in the pathogenesis of APECED. PMID:26903483

  16. Cost-effectiveness of three echinocandins and fluconazole in the treatment of candidemia and/or invasive candidiasis in nonneutropenic adult patients

    Grau S

    2015-10-01

    Full Text Available S Grau,1 JC Pozo,2 E Romá,2 M Salavert,3 JA Barrueta,4 C Peral,4 I Rodriguez,5 D Rubio-Rodríguez,6 C Rubio-Terrés6 1Hospital del Mar (IMIM, Barcelona, 2Hospital Universitario Reina Sofía, Córdoba, 3Hospital Universitario y Politécnico La Fe, Valencia, 4Pfizer SLU, Alcobendas, 5Trial Form Support, Madrid, 6Health Value, Madrid, Spain Objective: To estimate the cost-effectiveness of three echinocandins (anidulafungin, caspofungin, and micafungin and generic fluconazole in the treatment of nonneutropenic adult patients with candidemia and/or invasive candidiasis in intensive care units in Spain. Materials and methods: A decision-tree model was applied. The success and safety (hepatic and renal adverse effects of first-line treatments were obtained from meta-analyses and systematic reviews of clinical trials. In the case of failure, a second-line treatment (liposomal amphotericin B after the echinocandins, or one of the echinocandins after fluconazole was administered. The duration of the treatments (14 days total was established by a panel of clinical experts using the Delphi method and according to Infectious Diseases Society of America guidelines. The cost of the medications and renal toxicity were considered. Deterministic and probabilistic sensitivity analysis using Monte Carlo simulations were carried out. Results: The total cost of the treatment of candidemia and/or invasive candidiasis with anidulafungin, caspofungin, micafungin, and fluconazole was €5,483, €5,968, €6,231, and €2,088, respectively. Anidulafungin was the dominant treatment (more effective, less expensive compared to micafungin and caspofungin. The cost of achieving one more patient successfully treated with anidulafungin, caspofungin, and micafungin compared to fluconazole was €17,199, €23,962, and €27,339, respectively. The result remained stable, despite modification of the duration of the first-line and second-line treatments, as well as most of the

  17. Use of Quantitative Real-Time PCR To Study the Kinetics of Extracellular DNA Released from Candida albicans, with Implications for Diagnosis of Invasive Candidiasis

    Kasai, Miki; Francesconi, Andrea; Petraitiene, Ruta; Petraitis, Vidmantas; Kelaher, Amy M.; Kim, Hee-sup; Meletiadis, Joseph; Sein, Tin; Bacher, John; Walsh, Thomas J.

    2006-01-01

    Quantitative real-time PCR (qPCR) is considered one of the most sensitive methods to detect low levels of DNA from pathogens in clinical samples. To improve the design of qPCR for the detection of deeply invasive candidiasis, we sought to develop a more comprehensive understanding of the kinetics of DNA released from Candida albicans in vitro and in vivo. We developed a C. albicans-specific assay targeting the rRNA gene complex and studied the kinetics of DNA released from C. albicans alone, ...

  18. The Expression of Toll-like Receptor 2 and 4 mRNA in Local Tissues of Model of Oropharyngeal Candidiasis in Mice

    张少如; 李家文; 贾雪松; 邬炎卿

    2004-01-01

    To investigate the expression of Toll-like receptor (TLR) 2 and 4 mRNA in local tissues of model of oropharyngeal candidiasis in mice and to explore the potential role of TLR2 and TLR4 in earlier period of immune response, a murine model of oropharyngeal candidiasis inoculated by cotton wool balls saturated with candida albicans was established. Mice were sacrificed at the indicated time points and the oropharyngeal tissues were excised. The expression of TLR2 and TLR4 mRNA was detected by RT-PCR. The results showed that low level of TLR2/4 mRNA could be detected in oropharyngeal tissues, but they were markedly up-regulated 6 h after inoculation, peaking after12-24 h. Tissue TLR4 mRNA was gradually down-regulated 24-48 h, while TLR2 mRNA levels remained high up to the 72nd h. These data suggested that oropharyngeal infection of Candida albicans could result in up-regulation of TLR2/4 mRNA expression in local tissues, which might play important roles in earlier period of immune response.

  19. In Vitro Activity of Miltefosine against Candida albicans under Planktonic and Biofilm Growth Conditions and In Vivo Efficacy in a Murine Model of Oral Candidiasis.

    Vila, Taissa Vieira Machado; Chaturvedi, Ashok K; Rozental, Sonia; Lopez-Ribot, Jose L

    2015-12-01

    The generation of a new antifungal against Candida albicans biofilms has become a major priority, since biofilm formation by this opportunistic pathogenic fungus is usually associated with an increased resistance to azole antifungal drugs and treatment failures. Miltefosine is an alkyl phospholipid with promising antifungal activity. Here, we report that, when tested under planktonic conditions, miltefosine displays potent in vitro activity against multiple fluconazole-susceptible and -resistant C. albicans clinical isolates, including isolates overexpressing efflux pumps and/or with well-characterized Erg11 mutations. Moreover, miltefosine inhibits C. albicans biofilm formation and displays activity against preformed biofilms. Serial passage experiments confirmed that miltefosine has a reduced potential to elicit resistance, and screening of a library of C. albicans transcription factor mutants provided additional insight into the activity of miltefosine against C. albicans growing under planktonic and biofilm conditions. Finally, we demonstrate the in vivo efficacy of topical treatment with miltefosine in the murine model of oropharyngeal candidiasis. Overall, our results confirm the potential of miltefosine as a promising antifungal drug candidate, in particular for the treatment of azole-resistant and biofilm-associated superficial candidiasis. PMID:26416861

  20. Fluconazole therapy for treatment of invasive candidiasis in Intensive Care patients. Is it still valid from a pharmacological point of view?

    Mario Musu

    2014-01-01

    Full Text Available Fluconazole – antimycotic belonging to the first generation azoles – is widely used as treatment for invasive candidiasis and candidemia in numerous clinical settings as Neonatal Intensive Care Unit (NICU and adult Intensive Care Unit (ICU, as well as oncology, onco-hematology and solid organ transplantation. More recently use of antimycotics has spread to medical divisions, where fungal infections represent an emerging problem due to population’s ageing, malnourishment and important comorbidities. Fluconazole is effective against numerous Candida species, particularly against albicans, tropicalis and parapsilosis strains. On the other hand, C. krusei is intrinsically resistant to fluconazole and C. glabrata can be sensitive or resistant in a dose dependent fashion. Epidemiological variability is noteworthy and depends on the geographical location of the institution, the clinical setting, and the frequency and intensity of fluconazole employment for invasive candidiasis. In many ICUs fluconazole sensitive C. albicans is cultured in 50% of positive samples, while the remaining 50% show growth of variably sensitive fungal species, often resistant to fluconazole. Due to increasingly frequent emergence of resistant strains of Candida spp., American guidelines (IDSA in 2009, and European ones (ESCMID in 2012, recommended substitution of fluconazole with echinocandines as first line therapy in patients with severe disease, as defined by an APACHE II score greater than 15. Thus fluconazole must be limited to low risk cases, treatment of sensitive strains and de-escalation from echinocandin therapy, after microbiological diagnosis and drug resistance profile characterization.

  1. In vitro Susceptibility of Fluconazole, Clotrimazole and Toucrium Polium Smoke Product on Candida Isolates of Vaginal Candidiasis

    B Bonyadpour

    2009-07-01

    Full Text Available ABSTRACT: Introduction & Objective: It has been estimated that up to 75% of women in their child-bearing age have been affected by vulvovaginal candidiasis at least once in their life time. Almost 45% of women experience this infection two or more times. The antifungal azole group, in topical and oral forms, is the common way of therapy. Herbal products are often used for vulvovaginal therapy. Nowadays, Toucrium polium (TP products are being used as traditional medicine to reduce signs of Candida vaginitis. There is no study regarding to antifungal activity of TP smoke product in Iran. The aim of this study was to evaluate the In vitro activity of TP smoke product against Candida, isolated from women with Candida vaginitis, compared with antifungal drugs which are ordinary used to cure Candida vaginitis. Materials & Methods: The present study was conducted at the University of Medical Sciences of Shiraz in 1387 (2008. During seven months, samples were taken from 450 patients suffering from urogenital infections and 105 Candida vaginitis were detected. Germ tube test was used for identification of fungal species. TP smoke product was prepared in suitable potency. Antifungal activity of fluconazole, clotrimazole and TP product were evaluated by disk diffusion method. Sterile blank disks were loaded by TP smoke product in potency of 10-240 microliter/disk. Inhibition zone around the disks were measured and compared with each other. Results: 105 Candida species were isolated from the patients. Candida species were identified by germ tube test as Candida albicans 74 (70.5% and Candida non-albicans 31(29.5%.The mean of inhibition zone around the clotrimazole disks was 22±5.39 along with one case of resistance. Forty seven species had resistance to fluconazole while 94% and 55.2.% of all samples were sensitive to clotrimazole and fluconazole respectively. All of the clinical isolates and standard Candida species were sensitive to TP smoke product

  2. The Diagnosis and Treatment Measures of Recurrent Vulvovaginal Candidiasis%复发性外阴阴道念珠菌病的诊疗措施

    黄梅库

    2011-01-01

    目的:探索伊曲康唑治疗复发性外阴阴道念珠菌病的临床疗效.方法:选择我院2008年1月~2010年1月收治的复发性外阴阴道念珠菌病患者76例,将上述患者随机分为治疗组40例和对照组36例,观察两组之间的疗效.结果:治疗组1周后的总有效率为92.5%(37/40),对照组的总有效率为88.9%(32/36),两组之间总有效率比较无统计学意义(P >0.05).但是两组之间3个月(χ2=5.316,P<0.05)和6个月(χ2=11.740,P<0.01)复发率比较有统计学意义.结论:伊曲康唑短程冲击治疗联合硝酸咪康唑栓对复发性外阴阴道念珠菌病的治疗和预防复发有一定疗效,值得在临床推广应用.%Objective: To explore the clinical efficacy of treatment of recurrent vulvovaginal candidiasis by Itraconazole.Methods: To choose 76 cases treated patients with recurrent vulvovaginal candidiasis from January 2010 in our hospital, the patients were randomly divided into treatment group 36 cases and control group 40 cases, observed curative effect between two groups.Results: The total effective rate was 92.5% (37/40) in the treatment group after 7 days, the total effective rate was 88.9% (32/36) in the control group, and the total effective rate between the two groups was not significant (P>0.05). However, the recurrence rate was statistically significant in 3 months and 6 months between the two groups (χ2=5.316, P<0.05; χ2=11.74, P<0.01). Conclusion: There is certain curative effect of recurrent vulvovaginal candidiasis in treatment and prevention by the short-term-itraconazole-impact therapy combined Miconazole. It is worth applying in the clinical expansion.

  3. The Condition of Vulvovaginal Candidiasis Before Artificial Abortion%人工流产术前外阴阴道念珠菌病治疗的情况

    王界敏

    2016-01-01

    目的:研究分析人工流产手术前使用克霉唑阴道片治疗外阴阴道念珠菌病的效果。方法根据我院48例人工流产前外阴阴道念珠菌病患者来进行研究,观察组使用500 mg/d克霉唑阴道片治疗,对照组使用200 mg/d克霉唑阴道片治疗,对两组的治疗情况进行观察分析。结果两组的治疗有效率为,对照组54.67%,观察组83.33%,存在统计学差异性,P<0.05;观察组症状3天内缓解,对照组有8例3天后缓解,结果存在统计学差异,P<0.05。结论500 mg/d的克霉唑阴道片剂量能够有效治疗人工流产术前外阴阴道念珠菌病,安全性高,结合适当的护理可以促进患者的恢复。%Objective To study and analyze the artiifcial abortion operation before the use of clotrimazole vaginal tablets of vulvovaginal candidiasis. Methods 48 cases before artificial abortion vulva vaginal candidiasis patients, observation group use 500 mg/d clotrimazole vaginal tablets in the treatment, control group using 200 mg/d clotrimazole vaginal tablets in the treatment, the treatment of the two groups were observed and analyzed. Results Two groups of the effective rate of treatment was, control group 54.67%, observation group was 83.33%, had signiifcant difference (P<0.05). Symptoms in the observation group within 3 days of remission, control group had 8 cases , 3 days after the remission, the results of statistical difference (P<0.05). Conclusion Clotrimazole vaginal tablets dose to effective treatment of artiifcial abortion of vulvovaginal candidiasis, high safety, combined with the appropriate nursing can promote the patient's recovery.

  4. The resistance to fluconazole in patients with esophageal candidiasis Resistência ao fluconazol em pacientes com candidíase esofágica

    Ana Botler Wilheim

    2009-03-01

    Full Text Available CONTEXT: Esophageal candidiasis is often observed in patients with risk factors for its development and fluconazole is the therapeutic choice for the treatment of this disease. OBJECTIVES: To determine its frequency, by performing upper digestive endoscopy; to determine Candida species involved in its pathogenesis and verify their distribution according with the predisposing factors and to determine susceptibility to fluconazole in the samples. METHODS: From March 2006 to April 2007, all patients submitted to esophagogastroduodenoscopy at the Digestive Endoscopy Unit in the Oswaldo Cruz University Hospital, Recife, PE, Brazil, were eligible for the study. Samples were collected from patients who presented lesions consistent with esophageal candidiasis in order to identify Candida species and verify their susceptibility to fluconazole. The predisposing factors for the occurrence of esophageal candidiasis were described. RESULTS: Of 2,672 patients referred to upper endoscopy at the Digestive Endoscopy Unit, 40 (1.5% had endoscopic findings compatible with esophageal candidiasis. The average age was 49.1 years. Twenty one patients (52.5% were less than 50 years old, of which 82.6% were infected with HIV. Most of them (52.5% were males and 65.0% were inpatients. Diseases were identified in 90% of the patients and 21 (52.5% were HIV positive. Concerning endoscopic findings, severe forms of esophagitis were found in 50% of the patients with CD4 count CONTEXTO: A candidíase esofágica é comumente observada em pacientes com fatores de risco para seu desenvolvimento. OBJETIVOS:Determinar a freqüência da candidíase esofágica, por meio da endoscopia digestiva alta; identificar as espécies de Candida envolvidas na patogênese da candidíase esofágica e sua distribuição de acordo com o fator predisponente; determinar a susceptibilidade ao fluconazol nas amostras coletadas. MÉTODOS: De março de 2006 a abril de 2007, os pacientes submetidos a

  5. CANDIDIASIS ORAL EN PACIENTES VIH POSITIVOS. CLINICA DE INFECTOLOGIA, HOSPITAL OSCAR DANILO ROSALES ARGUELLO LEÓN, MARZO-JUNIO DE 2011.

    Espinoza Palma, Alicia Samanta; Paz Betanco, Manuel Ahmed; Espinoza Abaúnza, Róger

    2014-01-01

    La falta de estudios publicados en nuestro país en relación al tema, hace muy importante determinar la prevalencia de Candidiasis Oral, localizaciones más frecuentes y aparición de lesiones según tiempo de diagnóstico de VIH, en Pacientes atendidos en la Clínica de Infectología del Hospital Escuela Oscar Danilo Rosales Arguello de la ciudad de León. Posterior a aprobación por el comité de Ética Médica, UNAN León, se realizó estudio descriptivo de corte transversal, se recolectó información de...

  6. First characterization of Candida albicans by random amplified polymorphic DNA method in Nicaragua and comparison of the diagnosis methods for vaginal candidiasis in Nicaraguan women

    Bello Martha Darce

    2002-01-01

    Full Text Available A total of 106 women with vaginitis in Nicaragua were studied. The positive rate for the identification of Candida species was 41% (44 positive cultures out of 106 women with vaginitis. The sensitivity of microscopic examination of wet mount with the potassium hydroxide (KOH was 61% and 70% with Gram's stain when using the culture of vaginal fluid as gold standard for diagnosis of candidiasis. Among the 44 positives cultures, isolated species of yeast from vaginal swabs were C. albicans (59%, C. tropicalis (23%, C. glabrata (14% and C. krusei (4%. This study reports the first characterization of 26 C. albicans stocks from Nicaragua by the random amplified polymorphic DNA method. The genetic analysis in this small C. albicans population showed the existence of linkage disequilibrium, which is consistent with the hypothesis that C. albicans undergoes a clonal propagation.

  7. Detection of phospholipase activity of Candida albicans and non albicans isolated from women of reproductive age with vulvovaginal candidiasis in rural area

    S R Fule

    2015-01-01

    Full Text Available Background: Vulvovaginal candidiasis (VVC is most common accounting for 17 to 39% of symptomatic women. Both Candida albicans and non albicans Candida species are involved in VVC. Amongst various virulence factors proposed for Candida, extracellular phospholipases is one of the virulence factor implicated in its pathogenicity. With this background the present study was carried out to find the prevalence of different Candida species and to detect phospholipase producing strains isolated from symptomatic women with VVC. Materials and Methods: At least two vaginal swabs from 156 women of reproductive age with abnormal vaginal discharge were collected. Direct microscopy and Gram′s stained smear examined for presence of budding yeast and pseudo mycelia followed by isolation and identification of Candida species. Extracellular phospholipase activity was studied by inoculating all isolates on Sabouraud′s dextrose egg yolk agar (SDA medium. Results: Of the 156 women with curdy white discharge alone or in combination with other signs, 59 (37.82% women showed laboratory evidence of VVC. A total of 31 (52.54% women had curdy white discharge followed by 12 (20.33% with other signs and symptoms. C. albicans (62.59% and non albicans Candida (37.28% in a ratio of 1.68:1 were isolated. Of the 37 strains of C. albians 30 (81.08% showed the enzyme activity. Seventeen (56.66% strains showed higher Pz value of < 0.70 (++++. Conclusion: Although there may be typical clinical presentation of Candidiasis. all the patients did not show laboratory evidence of infection. Pregnancy was found to be major risk factor for development of VVC. C. albicans was prevalent species but non albicans species were also frequently isolated. Extracellular phospholipase activity was seen in C. albicans and not in non albicans Candida isolates.

  8. Efficacy of the clinical agent VT-1161 against fluconazole-sensitive and -resistant Candida albicans in a murine model of vaginal candidiasis.

    Garvey, E P; Hoekstra, W J; Schotzinger, R J; Sobel, J D; Lilly, E A; Fidel, P L

    2015-09-01

    Vulvovaginal candidiasis (VVC) and recurrent VVC (RVVC) remain major health problems for women. VT-1161, a novel fungal CYP51 inhibitor which has potent antifungal activity against fluconazole-sensitive Candida albicans, retained its in vitro potency (MIC50 of ≤0.015 and MIC90 of 0.12 μg/ml) against 10 clinical isolates from VVC or RVVC patients resistant to fluconazole (MIC50 of 8 and MIC90 of 64 μg/ml). VT-1161 pharmacokinetics in mice displayed a high volume of distribution (1.4 liters/kg), high oral absorption (73%), and a long half-life (>48 h) and showed rapid penetration into vaginal tissue. In a murine model of vaginal candidiasis using fluconazole-sensitive yeast, oral doses as low as 4 mg/kg VT-1161 significantly reduced the fungal burden 1 and 4 days posttreatment (P < 0.0001). Similar VT-1161 efficacy was measured when an isolate highly resistant to fluconazole (MIC of 64 μg/ml) but fully sensitive in vitro to VT-1161 was used. When an isolate partially sensitive to VT-1161 (MIC of 0.12 μg/ml) and moderately resistant to fluconazole (MIC of 8 μg/ml) was used, VT-1161 remained efficacious, whereas fluconazole was efficacious on day 1 but did not sustain efficacy 4 days posttreatment. Both agents were inactive in treating an infection with an isolate that demonstrated weaker potency (MICs of 2 and 64 μg/ml for VT-1161 and fluconazole, respectively). Finally, the plasma concentrations of free VT-1161 were predictive of efficacy when in excess of the in vitro MIC values. These data support the clinical development of VT-1161 as a potentially more efficacious treatment for VVC and RVVC. PMID:26124165

  9. 外阴阴道假丝酵母菌病临床治疗方法的选择%Selection of clinical treatment of vulvovaginal candidiasis

    周艳梅

    2014-01-01

    目的:探讨外阴阴道假丝酵母菌病的临床治疗方法。方法:2009年1月-2013年12月收治外阴阴道假丝酵母菌患者300例,年龄20~50岁,根据患者情况选择局部或全身应用抗真菌药物治疗,A 组:局部和全身联合用药治疗;B组:口服药物全身治疗;C组:阴道深处局部用药治疗。结果:治疗后总有效率A组、B组与C组比较差异有统计学意义(P<0.05),B 组与 C 组比较差异无统计学意义(P>0.05)。结论:局部和全身联合性治疗有协同增效的效果,为首选方法。%Objective:To investigate the clinical treatment of vulvovaginal candidiasis.Methods:300 cases with vulvovaginal candidiasis were selected from January 2009 to December 2013,age 20~50 years old.They were given the local or systemic application of antifungal treatment according to the situation of the patients.Group A:local and systemic combination treatment;group B:oral drug therapy;group C:vaginal depths of local drug therapy.Results:After the treatment,the difference of the total efficiency between the three groups was statistically significant(P0.05).Conclusion:The local and systemic combination treatment has the synergistic effect,so it can be the preferred treatment method.

  10. Analysis of Candida species causing vulvovaginal candidiasis%外阴阴道念珠菌病的致病菌群分析

    徐海萍; 王桂芝; 王君; 臧运书; 葛红芬; 王莹莹

    2013-01-01

    Objective To analyze Candida species causing vulvovaginal candidiasis in Qingdao and surrounding areas.Methods Vaginal discharge specimens were collected from 362 patients with suspected vulvovaginal candidiasis in the Affiliated Hospital of Qingdao University Medical College from May 2011 to November 2011,and subjected to routine fungal culture.The Candida isolates were identified by using Sabouraud dextrose agar,CHROMagar medium,germ tube test with serum,and API 20C AUX Clinical Yeast System.Results Totally,313 (86.46%) Candida strains were isolated from the specimens of 362 patients.Of these Candida strains,275 were identified as Candida albicans,38 as non-albicans Candida species,including 13 Candida glabrata strains,8 Candida parapsilosis strains,7 Candida tropicalis strains,5 Candida krusei strains,1 Candida lusitaniae strain,1 Candida dubliniensis strain,1 Rhodotorula mucilaginosa strain,1 Pichia ohmeri strain and 1 Trichosporon mucoides strain.Conclusions As far as vulvovaginal candidiasis is concerned,Candida albicans is still the most prevalent pathogen,and Candida glabrata appears to be the predominant species in pathogenic non-albicans Candida species.%目的 探讨青岛及周边地区外阴阴道念珠菌病致病菌的菌种特征.方法 采用常规念珠菌培养方法鉴定菌种,包括沙氏培养基,血清芽管实验,CHROMagar念珠菌显色培养基及API 20C AUX酵母菌鉴定系统.结果 2011年5~11月共收集362例妇科门诊患者的阴道分泌物,病原学分析显示,念珠菌阳性例数为313例,总感染率为86.46%,菌种构成分布为白念珠菌275株,光滑念珠菌13株,近平滑念珠菌8株,热带念珠菌7株,克柔念珠菌5株,葡萄牙念珠菌1株,都柏林念珠菌1株,粘质红酵母菌1株,奥默毕赤酵母菌1株,粘性丝孢酵母菌1株.结论 白念珠菌仍是外阴阴道念珠菌病的常见致病菌,非白念以光滑念珠菌为主.

  11. Treatment and prophylaxis of invasive candidiasis with anidulafungin, caspofungin and micafungin and its impact on use and costs - review of the literature

    Wilke MH

    2011-04-01

    Full Text Available Abstract Invasive fungal infections are on the rise. Echinocandins are a relatively new class of antifungal drugs that act by inhibition of a key enzyme necessary for integrity of the fungal cell wall. Currently there are three available agents: caspofungin, micafungin and anidulafungin. While the individual echinocandin antifungals have a different spectrum of licensed indications, basically all of them are available for the treatment of candidemia and invasive candidiasis. Antifungal treatment modalities basically include in therapy for suspected or proven infection and prophylaxis. All three drugs are comparatively expensive. Therefore a systematic review of the literature was performed to investigate the following aspects: • General aspects of cost-effectiveness in the treatment of invasive fungal infections • Cost-effectiveness of the treatment with the above-mentioned antifungals • Cost-effectiveness in two settings: therapy and prophylaxis Early initiation of antifungal therapy, adjustment after availability of microbiological results, duration of therapy, success and occurrence of severe complications (e.g renal failure are the most important cost drivers in antifungal therapy. Considering the specific antifungals, for caspofungin the best evidence for cost-effectiveness is found in treatment of invasive candidiasis and in empiric therapy of suspected infections. Favourable economic data are available for micafungin as a cost-effective alternative to LAmB for prophylaxis in patients with hematopoietic stem cell transplantation (HSCT. For anidulafungin, cost-effectiveness was demostrated in a pharmacoeconomic model. Net savings - yet not significant - were observed in a retrospective chart review of 234 patients. Generally, however, most analyses are still based on pharmacoeconomic modelling rather than direct analysis of trial data or real-life clinical populations. As an overall conclusion, using caspofungin, micafungin, or

  12. In vivo analysis of helper T cell responses in patients with autoimmune polyendocrinopathy - candidiasis - ectodermal dystrophy provides evidence in support of an IL-22 defect.

    Laakso, Sini M; Kekäläinen, Eliisa; Heikkilä, Nelli; Mannerström, Helga; Kisand, Kai; Peterson, Pärt; Ranki, Annamari; Arstila, T Petteri

    2014-12-01

    Autoimmune polyendocrinopathy - candidiasis - ectodermal dystrophy (APECED) is caused by mutations in the Autoimmune regulator (AIRE) gene and is associated with neutralizing anti-cytokine autoantibodies. We have used an in vivo challenge model to analyze antigen-specific CD4(+) T cell responses. Bacille Calmette-Guérin (BCG)-vaccinated patients and controls were injected tuberculin intradermally, skin blisters were induced by suction on the indurations and on unexposed skin, and the infiltrating cells harvested. The patients had a quantitatively normal CD4(+) T cell response and no significant abnormalities in the expression of T helper type (Th) 1- or Th2-related genes. The expression of interleukin (IL)-22, in contrast, was lower in the patients. Two patients, both with a pre-existing ocular keratopathy, experienced a relapse of keratoconjunctivitis, suggesting a possible immunological basis for this APECED component. Our in vivo data are compatible with a selective IL-22 defect in the activated CD4(+) T cells of APECED patients, affecting also unexposed skin in steady-state conditions. PMID:24957984

  13. Oropharyngeal Candidiasis in HIV Infection: Analysis of Impaired Mucosal Immune Response to Candida albicans in Mice Expressing the HIV-1 Transgene

    Louis de Repentigny

    2015-06-01

    Full Text Available IL-17-producing Th17 cells are of critical importance in host defense against oropharyngeal candidiasis (OPC. Speculation about defective Th17 responses to oral C. albicans infection in the context of HIV infection prompted an investigation of innate and adaptive immune responses to Candida albicans in transgenic mice expressing the genome of HIV-1 in immune cells and displaying an AIDS-like disease. Defective IL-17 and IL-22-dependent mucosal responses to C. albicans were found to determine susceptibility to OPC in these transgenic mice. Innate phagocytes were quantitatively and functionally intact, and individually dispensable for control of OPC and to prevent systemic dissemination of Candida to deep organs. CD8+ T-cells recruited to the oral mucosa of the transgenic mice limited the proliferation of C. albicans in these conditions of CD4+ T-cell deficiency. Therefore, the immunopathogenesis of OPC in the context of HIV infection involves defective T-cell-mediated immunity, failure of crosstalk with innate mucosal immune effector mechanisms, and compensatory cell responses, which limit Candida infection to the oral mucosa and prevent systemic dissemination.

  14. Adjuvant antifungal therapy using tissue tolerable plasma on oral mucosa and removable dentures in oral candidiasis patients: a randomised double-blinded split-mouth pilot study.

    Preissner, Saskia; Kastner, Isabell; Schütte, Eyke; Hartwig, Stefan; Schmidt-Westhausen, Andrea Maria; Paris, Sebastian; Preissner, Robert; Hertel, Moritz

    2016-07-01

    Extended use of antimycotics in oral candidiasis therapy gives rise to problems related to fungal drug resistance. The aim of this pilot study was to investigate the efficacy of tissue tolerable plasma (TTP) in denture stomatitis patients. It was hypothesised that (I): erythema and (IIa): complaint remission would be accelerated and (IIb): colony forming unit (CFU) reduction would be improved. The halves of the upper jaws of eight patients were randomly assigned to control (nystatin, chlorhexidine and placebo treatment) and test sides (nystatin, chlorhexidine and TTP administered six times each 7 days). The patients and the investigators, who were different from the therapists, were both blinded. Compared to the control sides, the erythema surface was reduced significantly more extensively on the test sides between 2 and 6 weeks of antifungal therapy (P ≤ 0.05). Visual analogue scale values and the frequency of moderate or heavy growth of Candida post-treatment did not differ significantly between both sides (P > 0.05). The primary hypothesis was confirmed, which may be interpreted as an accelerated remission. As drug therapy is usually limited to the time in which signs of infection are present, TTP might help reducing antifungal use. Even though the secondary hypotheses were not confirmed, persistence of Candida might be only colonisation. PMID:26932256

  15. Expression and Significance of NF-κB, IL-1β and COX-2 in the Murine Model of Estrogen-dependent Experimental Vulvovaginal Candidiasis

    Xue-rong CHEN; Ya-li LIU; Dun-zhen XIAO; Jun GAO

    2007-01-01

    Objective To investigate the possible role of estrogen in the pathogenesis of vulvovaginal candidiasis(VVC).Methods Estrogen-dependent experimental murine model of C. albicans vaginal infection was established by injecting subcutaneously with estradiol benzoate and then 5 × 106 stationary-phase C. albicans blastoconidia was inoculated intravaginally to mice (group EI),and other 3 groups were set up: estrogen-treated but not infected (group E) ;estrogen-untreated but infected (group Ⅰ);normal control (group C).The dynamic change of colony-forming unit (CFU) of cervivovaginal lavage fluid was observed. Vaginal tissues at different time points (d 2,d 4,d 7 and d 14) after inoculation of C.albicans were obtained.In situ hybridization staining was used to detect expression of on d 4 and d 7 (P<0.01).Conclusions In the murine model of estrogen-dependent experimental VVC,estrogen promotes the infection establishment by up-regulating expression of CO X-2 via activating NF-κB signal pathway,and the high expression of COX-2 promoted by the interaction of IL-1β and NF-κB after infection formation was involved in persistence of infection.

  16. The Correlation Between Candida Colonization of Distinct Body Sites and Invasive Candidiasis in Emergency Intensive Care Units: Statistical and Molecular Biological Analysis.

    Li, Zhen; Jiang, Cen; Dong, Danfeng; Zhang, Lihua; Tian, Yuan; Ni, Qi; Mao, Enqiang; Peng, Yibing

    2016-08-01

    Both statistical and molecular biological methods were used to evaluate the association between Candida colonization of different body sites and invasive candidiasis (IC) and analyse the potential infection sources of IC. Candida surveillance cultures from the urine, sputum, rectum and skin were performed on patients admitted to an emergency intensive care units (EICU) of a tertiary care hospital in Shanghai, China, from February 2014 to January 2015. Specimens were collected once a week at admission and thereafter. The patients' clinical data were collected, and Candida isolates were genotyped using polymorphic microsatellite markers. A total of 111 patients were enrolled. Patients with positive urine (23.3 vs. 2.5 %, p = 0.001) and rectal swab (13.6 vs. 0 %, p = 0.010) cultures were more likely to develop IC. However, the risk for IC was not significantly different among patients with and without respiratory (10.0 vs. 5.8 %, p = 0.503) and skin (33.3 vs. 6.5 %, p = 0.056) colonization. Gene microevolution frequently occurred at rectal swab and urine sites, and IC with possible source of infection was caused by rectal isolates (2/7), urine isolates (4/7) and sputum isolate (1/7).The colonization of gut and urinary tract maybe more relevant indicators of IC, which should be taken into consideration when selecting practical body sites for Candida surveillance cultures. PMID:26886444

  17. 外阴阴道假丝酵母菌病的诊治进展%Diagnosis and Treatment Advancement of Vulvovaginal Candidiasis

    吴文湘

    2011-01-01

    Vulvovaginal Candidiasis (VVC)is a common gynecologic disease,especially in women of childbearing age.VVC usually is caused by Candida albicans, but occasionally is caused by non-albicans Candida. The diagnosis can be made in a woman who has signs and symptoms of vaginitis when mycologic laboratory examination is positive.American Center for Disease Control and Prevention has renewed the treatment guidelines of VVC in 2010. VVC is treated with a variety of anti-fungal drugs. Individual treatment should be recognized according to different classification of VVC. Other treatments about immunotherapy and lactobacillus administration are still at the research stage.%外阴阴道假丝酵母菌病(VVC)是育龄女性的常见病和多发病.其病原体分为白色假丝酵母菌和非白色假丝酵母菌两大类,以白色假丝酵母菌为主.诊断主要依靠临床症状、体征和实验室检查.2010年美国疾病控制中心更新了VVC的诊治规范,根据VVC的不同分类,选择个体化的抗真菌治疗.其他治疗如免疫治疗和乳酸杆菌治疗等仍处于研究阶段.

  18. Oropharyngeal Candidiasis in HIV Infection: Analysis of Impaired Mucosal Immune Response to Candida albicans in Mice Expressing the HIV-1 Transgene.

    de Repentigny, Louis; Goupil, Mathieu; Jolicoeur, Paul

    2015-01-01

    IL-17-producing Th17 cells are of critical importance in host defense against oropharyngeal candidiasis (OPC). Speculation about defective Th17 responses to oral C. albicans infection in the context of HIV infection prompted an investigation of innate and adaptive immune responses to Candida albicans in transgenic mice expressing the genome of HIV-1 in immune cells and displaying an AIDS-like disease. Defective IL-17 and IL-22-dependent mucosal responses to C. albicans were found to determine susceptibility to OPC in these transgenic mice. Innate phagocytes were quantitatively and functionally intact, and individually dispensable for control of OPC and to prevent systemic dissemination of Candida to deep organs. CD8+ T-cells recruited to the oral mucosa of the transgenic mice limited the proliferation of C. albicans in these conditions of CD4+ T-cell deficiency. Therefore, the immunopathogenesis of OPC in the context of HIV infection involves defective T-cell-mediated immunity, failure of crosstalk with innate mucosal immune effector mechanisms, and compensatory cell responses, which limit Candida infection to the oral mucosa and prevent systemic dissemination. PMID:26110288

  19. Transforming growth factor-β1 sustains the survival of Foxp3(+) regulatory cells during late phase of oropharyngeal candidiasis infection.

    Bhaskaran, N; Quigley, C; Weinberg, A; Huang, A; Popkin, D; Pandiyan, P

    2016-07-01

    As CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) play crucial immunomodulatory roles during infections, one key question is how these cells are controlled during antimicrobial immune responses. Mechanisms controlling their homeostasis are central to ensure efficient protection against pathogens, as well as to control infection-associated immunopathology. Here we studied how their viability is regulated in the context of mouse oropharyngeal candidiasis (OPC) infection, and found that these cells show increased protection from apoptosis during late phase of infection and reinfection. Tregs underwent reduced cell death because they are refractory to T cell receptor restimulation-induced cell death (RICD). We confirmed their resistance to RICD, using mouse and human Tregs in vitro, and by inducing α-CD3 antibody-mediated apoptosis in vivo. The enhanced viability is dependent on increased transforming growth factor-β1 (TGF-β1) signaling that results in upregulation of cFLIP (cellular FLICE (FADD-like IL-1β-converting enzyme)-inhibitory protein) in Tregs. Protection from cell death is abrogated in the absence of TGF-β1 signaling in Tregs during OPC infection. Taken together, our data unravel the previously unrecognized role of TGF-β1 in promoting Treg viability, coinciding with the pronounced immunomodulatory role of these cells during later phase of OPC infection, and possibly other mucosal infections. PMID:26530137

  20. 两性霉素B对外阴阴道假丝酵母菌病抗真菌后巩固治疗效果观察%Amphotericin B therapy in consolidating antifungal treatment effects of vulvovaginal candidiasis

    王玉梅; 张斌

    2014-01-01

    目的:分析外阴阴道假丝酵母菌患者的临床有效治疗方法。方法:选取外阴阴道假丝酵母菌患者共104例作为研究对象,随机分为两组。其中对照组使用克霉唑治疗,实验组使用两性霉素B治疗,观察比较两组患者疗效。结果:实验组患者疼痛、阴道充血水肿改善高于对照组,P<0.05,差异有统计学意义;两组患者瘙痒症状、分泌物改善比较差异不明显,P>0.05,差异无统计学意义。结论:对外阴阴道假丝酵母菌患者使用两性霉素B治疗具有较好的效果,是一种有效的治疗方案。%Objectives:To explore clinically effective treatment for patients with vulvovaginal candidiasis. Methods:104 patients with vulvovaginal candidiasis were selected and randomly divided into two groups.Control group and experimental group adopted clotrimazole and amphotericin B therapy respectively;efficacy of the two groups were compared.Results:As for improvement in pain,vaginal congestion and edema of experimental group was higher than control group,P0.05.Conclusion:Amphotericin B therapy is of effective efficiency for patients with vulvovaginal candidiasis.

  1. A randomized clinical trial of the efficacy and safety of terconazole vaginal suppository versus oral fluconazole for treating severe vulvovaginal candidiasis.

    Li, Ting; Zhu, Yuxia; Fan, Shangrong; Liu, Xiaoping; Xu, Huicong; Liang, Yiheng

    2015-06-01

    Terconazole is a new, broad-spectrum, triazole antifungal agent. The aim of this study was to compare the efficacy and safety of a 6-day course of a terconazole vaginal suppository (80 mg) with two doses of oral fluconazole (150 mg) for the treatment of severe vulvovaginal candidiasis (SVVC). In this prospective, randomized case-control study, 140 consecutive patients with SVVC were enrolled at the Department of Obstetrics and Gynecology of Peking University Shenzhen Hospital from July 1, 2013, through June 31, 2014. Patients with SVVC, initially at a 1:1 ratio, were randomly assigned to receive treatment with either the terconazole vaginal suppository or oral fluconazole. The patients had follow-up visits at 7-14 days and 30-35 days following the last dose of therapy. The clinical cure rates in the terconazole group and the fluconazole group were, respectively, 81.0% (47/58) and 75.8% (50/66) at follow-up day 7-14 and 60.3% (35/58) and 56.1% (37/66) at day 30-35. The mycological cure rates in the two groups were, respectively, 79.3% (46/58) and 71.2% (47/66) at follow-up day 7-14 and 62.1% (36/58) and 53.0% (35/66) at day 30-35 (P > .05 for all). Local irritation was the primary adverse event associated with terconazole, whereas systemic side effects were associated with fluconazole; however, these effects were minimal. This study demonstrated that a terconazole vaginal suppository (80 mg daily for 6 days) was as effective as two dose of oral fluconazole (150 mg) in the treatment of patients with SVVC; as such, terconazole could be a choice for therapy of this disorder. PMID:25877666

  2. 外阴阴道假丝酵母菌病治疗的研究进展%Progress on the Treatment of Vulvovaginal Candidiasis

    夏艳

    2011-01-01

    外阴阴道假丝酵母菌病(VVC)是育龄期妇女的常见病.针对VVC的治疗方法主要有局部用药和口服用药,两种方法均有效.大量抗真菌药物的出现使选择用药出现争论.一般认为,唑类是首选用药,制霉菌素、两性霉素B和硼酸是二线用药,乳酸杆菌活菌制剂、精油疗法、抗过敏药为辅助用药.非传统疗法可治疗较顽同的病例.治疗中也需要考虑其他一些重要因素,包括妊娠、月经、药物敏感性、个人喜好等.%Vulvovaginal candidiasis (VVC)is a common disease, occurring mostly in women of childbearing age. Currently available options for the treatment of VVC include local and oral therapy. Both alternatives are effective. The availability of numerous antifungal drugs for topical administration makes the selection quite challenging. Now it is generally considered that azoles is the preference, with nystatin, amphotericin B and boric acid considered as secondline alternatives, with lactobacillus, oils and antihistamine as adjuvant alternatives. Unconventional approaches may also be regarded as suitable in patients refractory to usual treatments. When treatment of VVC, some other important issues should be taken into consideration, such as pregnancy, menopause, drug hypersensitivity, women′s preference and so on.

  3. 复发性外阴阴道假丝酵母菌病发病机制的研究进展%An analysis of the factors contributing to recurrent vulva and vagina candidiasis

    徐倩倩; 宁玉梅

    2013-01-01

    复发性外阴阴道假丝酵母菌病(Recurrent candidiasis of vulva and vagina,RVVC)病因复杂,主要原因有机体免疫防御机制的变化,宿主局部因素,假丝酵母菌的菌群多样性变化等.认识、了解及掌握上述因素,对临床工作者有效防治RVVC起着非常重要作用.

  4. The study of the lectin level in healthy population, vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC) groups%外阴阴道念珠菌病、复发性外阴阴道念珠菌病及健康人群甘露糖结合凝集素水平的分析研究

    刘格; 王薇华; 孙静; 马雪枫; 陈爱群

    2013-01-01

    Objective To value the levels of mannose lectin in healthy population,vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC) groups.Method All the VVC and RVVC cases were given standardized treatment.Compared with healthy population,the MBL levels before and after treatment were observed in these two groups.Results The MBL levels of vvc group,RVVC group,healthy group were arranged from high to low numerically.But there was no significant difference among groups or within every group before and after treatment (P > 0.05).There were 14% (5/43) cases of recurrence in VVC group and 48% (13/30) cases of recurrence in RVVC group,respectively.There was no significant differecne of recurrence between VVC and RVVC groups (P > 0.05).Conclusion The level of MBL is higher numerically in VVC group than the level in RVVC group.The recurrence in both groups is consistet with the previously literature.The VVC patients with MBL level higer than 0.8 ng/mL seems to have lower risk of suffering RVVC.%目的 观察健康人群、外阴阴道念珠菌病(VVC)和复发性外阴阴道念珠菌病(RVVC)的甘露糖结合凝集素水平.方法 收集健康人群、VVC和RVVC病例,给予规范化治疗,观察治疗前、后各组MBL水平的变化,并进行随访.结果 发现各组的MBL水平VVC组>RVVC组>健康组,但各组之间及各组本身治疗前后的MBL差异无显著性意义(P>0.05).治疗后VVC组复发者5例,占14%,RVVC组复发者13例,达到48%,两组复发者与未复发者的MBL水平,经t检验无显著性差异(P>0.05).结论 MBL的水平在VVC患者中的水平高于RVVC患者,两者的复发率基本与既往文献报道一致,对于VVC患者来说,MBL水平高于0.8 ng/mL似乎患RVVC的风险稍小些.

  5. The Research of Medicine Treatment on Recurrent Vulvovaginal Candidiasis%复发性外阴阴道念珠菌病的药物治疗研究进展

    武璁; 樊尚荣

    2011-01-01

    外阴阴道念珠菌病(VVC)是由念珠菌引起的常见感染性疾病,妇女1年内发作4次或以上称为复发性外阴阴道念珠菌病(RVVC).RVVC是近年来VVC治疗的难点之一,目前国内外尚无理想的治疗方案,其治疗原则包括强化治疗和维持治疗,本文综述了伊曲康唑、氟康唑、咪康唑、克霉唑和乳酸杆菌等药物治疗RVVC的效果及研究进展.%Vulvovaginal candidiasis is a common infection caused by Candida.Recurrent vulvovaginal candidiasis ( RVVC ) means four or more episodes of VVC in one year.There are no favorable treatments for RVVC in China.The therapeutic principle contains intensive therapy and maintenance therapy.This review summarized the effectiveness and research progress of itraconazole, fluconazole, miconazole, clotrimazole and lactobacillus in the treatment of RVVC.

  6. Species profiles of pathogens from 1366 superficial candidiasis cases in Shanghai%上海地区1366例浅部念珠菌病致病菌种分析

    冯晓博; 凌波; 杨桂梅; 余霞; 任大明; 姚志荣

    2012-01-01

    目的 分析上海地区1366例浅部念珠菌病的致病菌种构成及分布情况.方法 对1366例儿童与成人浅部念珠菌病的致病菌种进行菌种鉴定:采用科玛嘉平板、API20C AUX等鉴定手段常规鉴定;采用Pal平板、木糖同化试验、45℃生长试验鉴定都柏林念珠菌;采用分子鉴定方法鉴定近年发现的新种,包括Candida orthopsilosis、Candida metapsilosis、Candida fermentati、Candida nivariensis、Candida bracarensis.对致病菌种的构成及分布情况进行分析.结果 1366例浅部念珠菌病的主要致病菌为白念珠菌(占79.0%),排在2~4位的依次为近平滑念珠菌(9.5%)、热带念珠菌(2.9%)和季也蒙念珠菌(1.9%),致病株中包括念珠菌新种Candida orthopsilosis(2株)和Candida metapsilosis(4株);儿童患者与成人患者的致病菌种构成差异有统计学意义(x2=196.46,P< 0.01),其中非白念珠菌分离率分别为14.4%和45.8%.结论 白念珠菌仍为浅部念珠菌病的主要致病菌;新种Candida orthopsilosis和Candida metapsilosis可引起浅部念珠菌病.与儿童病例相比,成人病例中非白念珠菌的分离比例明显上升.%Objective To analyze the composition and distribution of pathogens from 1366 superficial candidiasis cases in Shanghai.Methods Candida species identification was carried out for 1366 adults or children with superficial candidiasis by using CHROMagar Candida plates,API20C AUX system,etc.Pal's agar,Xylose assimilation and the test for growth at 45 ℃ were utilized to differentiate Candida dubliniensis.Newly identified pathogenic Candida species including Candida orthopsilosis,Candida metapsilosis,Candida fermentati,Candida nivariensis and Candida bracarensis were differentiated by molecular biological methods.Finally,the composition and distribution of pathogens in superficial candidiasis cases were statistically analyzed.Results A total of 1366 Candida strains,included 2 Candida orthopsilosis strains and 4

  7. Treatment for vulvovaginal candidiasis in pregnant women%妊娠合并外阴阴道念珠菌病治疗研究

    杨晓庆

    2012-01-01

    目的 探讨克霉唑用于治疗妊娠合并外阴阴道念珠菌病(vulvovaginal eandidiasis,VVC)的疗效和安全性.方法 138例妊娠合并VVC患者随机分为克霉唑组与制霉菌素对照组,其中克霉唑组采取阴道放置克霉唑片联合外阴涂抹克霉唑软膏方法进行治疗,对照组采用阴道放置制霉菌素片治疗方法,分别于停药1周、4周后来院复查,对两组临床疗效进行比较.结果 ①停药1周和4周后,克霉唑组治愈率分别为50.72%、46.38%,总有效率分别为92.75%、95.65%;对照组停药1周和4周后,治愈率分别为33.33%、28.89%,总有效率分别为85.51%、88.41%;克霉唑组的治愈率与总有效率均大于对照组,但两组之间总有效率差异无统计学意义(P>0.05),而治愈率差异有统计学意义(P<0.05).②停药4周后,克霉唑组患者外阴阴道瘙痒、阴道分泌物增多以及排尿痛的症状缓解率分别为94.2%、95.65%和92.75%,均高于对照组85.5%、91.3%和91.3%的各症状缓解率,但两组之间差异均无统计学意义(P>0.05).③克霉唑组患者平均治愈时间为(10.37 ±1.19)d,少于对照组(13.65 ±0.87)d的平均治愈时间,两组间差异具有统计学意义(P<0.05).④经过治疗,克霉唑组念珠菌阳性率从100%显著减少为4.35% (P<0.01),对照组念珠菌阳性率从100%显著减少为10.14% (P<0.01),克霉唑组阳性率低于对照组,但两组之间差异无统计学意义(P>0.05).⑤克霉唑组与对照组患者均情况良好,无异常症状,且胎儿心律正常.结论 克霉唑与制霉菌素均可安全、有效治疗妊娠合并VVC,但使用克霉唑起效更迅速,治愈效果更好,操作更简便,疗效略优于制霉菌素,值得进一步推广.%Objective To explore the therapeutic effect and security of clotrimazole on pregnancy and vulvovaginal candi-diasis (VVC). Methods Totally 138 pregnant women with VVC were randomly divided into two groups. The

  8. 外阴阴道念珠菌病的病因研究及体外药敏检测%Analysis on the pathogens and fungi susceptibility test in vitro of patients with vulvovaginal candidiasis

    江爱萍; 黄剑清; 蔡蕾; 徐明

    2014-01-01

    Objective To analyze the pathogens of Vulvovaginal candidiasis in FuZhou. Methods 332 strains i-solated from vaginal secretions of 661 patients with vulvovaginal candidiasis in our department were identified with CHROMagar candida chromogenic culture medium,YBC method and API20C AUX. The susceptibility test of 4 anti-fungal drugs was tested by ROSCO disk diffusion method. Results The total infection rate was 69. 45%. In 339 strains of candida albicans, there were 288 stains of candida albicans ( 85%) , 23 stains of candida glabrata (6. 8%), 12 strains of candida tropicalis (3. 54%), 7 strains of candida krusei (2%), 4 strains of candida parap-silosis (1. 18%), 4 strains of Candida glabrata (1. 18%) and 1 strain of saccharomyces cerevisiae (0. 29%). Conclusion The major pathogens of vulvovaginal candidiasis is candida albicans, candida glabrata and candida tropi-calis in Fuzhou.%目的:了解福州地区阴道念珠菌病的病因、致病菌的菌种特征。方法采用科玛嘉念珠菌显色培养基和YBC鉴定卡及API-20C AUX酵母菌鉴定系统对门诊就诊661例患者阴道分泌物分离到的332株致病真菌进行鉴定,并采用ROSCO纸片扩散法对4种抗真菌药进行药物敏感检测。结果总感染率为69.45%共分离菌种6种。339株念珠菌,其中白念珠菌为288株占85%,光滑念珠菌23株占6.8%,热带念珠菌12株占3.54%,克柔念珠菌7株占2%,近平滑念珠菌4株占1.18%,光滑假丝酵母菌4株占1.18%,酿酒酵母菌1株占0.29%。结论本地区念珠菌性阴道炎主要的病原菌以白色念珠菌为主,其次是光滑念珠菌、热带念珠菌。

  9. 克霉唑联合苦参凝胶治疗重度外阴及阴道假丝酵母菌病%Treatment of severe vulvovaginal candidiasis with clotrimazde combined with gelatin kushen

    陈炳锦; 葛亚娟; 朱芳; 朱丽均; 陈亚萍

    2008-01-01

    目的 探讨克霉唑联合苦参凝胶治疗重度外阴、阴道假丝酵母茵病的临床治疗效果和药物不良反应.方法 98例外阴、阴道假丝酵母茵病患者采用随机单盲方法,分为克霉唑组34例、苦参凝胶组33例、克霉唑加苦参凝胶组31例,治疗后7d和月经后5d评估治疗效果.结果 克霉唑组临床治愈率和真菌清除率稍高于苦参凝胶组,但无统计学意义(P>0.05),克霉唑加苦参凝胶组在治疗后第7天和月经后第5天均明显优于其他2组(P<0.05).3组治疗耐受性好且均无严重药物不良反应发生.结论 克霉唑与苦参凝胶联合应用显著提高治疗效果,在治疗过程中未见严重局部和全身的药物不良反应,值得临床推广.%Objective To evaluate the therapeutic effect and safety of gelatin Kushen in the treatment of severe vulvovaginal candidiasis.Methotis Ninty.eight patients of severe vulvovaginal candidiasis were treated randomly with clotrimazole,gelatin Kushen or elotrimazole combined with gelatin Kushen.The therapeutic effect and adverse reaction were observed at 7 days after treatment and 5 days after menstruation.Results The effective rates were similar between elotrimazole and gelatin Kushen(P>0.05).Compared with single drug.the effective rate of clotrimazole combined with gelatin Kushen wal5 significantly increased(P<0.05).No adverse reaction Was found.Conclusion Clotrimazole combined with gelatin Kushen was effective and safe in the treatment of severe v'ulvovaginal candidiasis.

  10. 氟康唑胶囊治疗复发性外阴阴道念珠菌病的疗效观察%The Cinical effect of Di.ucan in the Treatment of Recurrent Vulvovaginal Candidiasis

    周建华

    2012-01-01

    目的 观察氟康唑胶囊疗复发性外阴阴道念珠菌病的疗效,探讨临床价值.方法 收集本院200 例复发性外阴阴道念珠菌病患者的临床资料,进行回顾性分析;随机分为2组,对照组(n=100) 给予口服氟胞嘧啶片(剂量150mg/ 次,1 次/d,连用7d) 治疗,观察组(n=100) 给予口服氟康唑胶囊(剂量150mg/ 次,1 次/周,连用两周)治疗,对比两组治疗效果.结果 所有患者分别于治疗后4周、6个月、12 个月进行复查,对比同期观察组与对照组的治疗效果,观察组的治疗显效率明显优于对照组,差异有显著性(P<0.05).结论 氟康唑胶囊治疗复发性外阴阴道念珠菌病,疗效确切、可缩短病程,具有临床推广价值.%Objective: To observe the treatment effect of diflucan in the treatment of recurrent vulvovaginal candidiasis and to explore the clinical value. Methods: 200 patients with recurrent vulvovaginal candidiasis were ramdomly divided into two groups. The control group (n=100) took the flucytosine tablets (150mg-dose once daily,7days). The observe group (n=100) took an oral diflucan(150mg-dose once a week). Evaluate the efficiency of the two groups after the treatment ended in 4 weeks, 6 months and 12 months. Results: The clinical treatment efficiency of the observe group was much better than the control group (P<0.05) in the same period after the treatment finished. Conclusion: Diflucan capsules treatment of recurrent vulvovaginal candidiasis, efficacy, may shorten the course, with the clinical value.

  11. Clinical Gyno-Daktarin joint Medical Ozone Treatment of Vulvovaginal Candidiasis Effect%达克宁栓联合医用臭氧治疗霉菌性阴道炎的临床疗效探讨

    周丽萍

    2015-01-01

    Objective To explore the clinical efficacy of medical ozone treatment of vulvovaginal candidiasis Gyno-Daktarin Suppository. Methods December 2013 - December 2014 period in our hospital 126 cases of vulvovaginal candidiasis pa-tients were randomly divided into two groups (n=63) underwent Gyno-Daktarin joint medical ozone treatment, the control group (63 cases) underwent Gyno-Daktarin simple drug treatment, observed and compared clinical efficacy and recurrence rate. Results The total effective rate was 95.2%, significantly higher than the 77.8%,χ2=8.23, P<0.01;observation group, the recurrence rate was 6.7%, significantly lower than the 26.5%in the control group,χ2=8.08, P<0.01. Conclusion Gyno-Dak-tarin be combined with medical ozone treatment of vulvovaginal candidiasis, which can effectively improve the clinical symptoms, and can reduce relapse.%目的:探究达克宁栓联合医用臭氧治疗霉菌性阴道炎的临床疗效。方法整群选择2013年12月—2014年12月期间该站收治的126例霉菌性阴道炎患者,随机平均分为观察组(63例)行达克宁栓联合医用臭氧治疗,对照组(63例)行达克宁栓单纯药物治疗,观察对比两组临床疗效及复发率。结果观察组临床总有效率为95.2%,明显高于对照组的77.8%,χ2=8.23,P<0.01;观察组复发率为6.7%,明显低于对照组的26.5%,χ2=8.08,P<0.01。结论对霉菌性阴道炎患者予以达克宁栓联合医用臭氧治疗,可有效改善临床症状,且可减少病情复发。

  12. Treatment of vulvovaginal candidiasis in pregnancy by live preparation of lactobacillus:an observation%定君生治疗妊娠期外阴阴道假丝酵母菌病的观察

    王小汤

    2012-01-01

    Objective To explore the clinical effect of live preparation of lactobacillus and amphotericin B in treatment of vulvovaginal candidiasis in pregnancy, and provide a reference for drug safety during pregnancy. Methods 116 cases of vulvovaginal candidiasis in pregnancy were divided into control group and treatment group. The patients of control group were given amphotericin B, while the treatment group were given live preparation of lactobacillus on the basis of control group medication. The effect and vaginal discharge were evaluated at 10 days, 30 days and 60 days after treatment. Results After treatment for 60 days, the effective rate of treatment group was higher than in the control group (94. 82% vs 82. 76%, P < 0. 05); there was a significant difference in relapse rate (12. 07% vs 29. 31 %, P < 0. 05). Conclusion Live preparation of lactobacillus and amphotericin B have long-lasting curative effect and low relapse rate in the treatment of vulvovaginal candidiasis in pregnancy.%目的 探讨定君生联合两性霉素B对妊娠期外阴阴道假丝酵母菌病的临床疗效,为妊娠期的安全用药提供参考.方法 选取116例妊娠期外阴阴道假丝酵母菌病患者,随机分为对照组和治疗组,对照组单纯给予两性霉素B,治疗组在对照组的基础上给予定君生,分别于治疗后10、30和60 d对患者的临床疗效和阴道分泌物情况进行评价.结果 治疗后60 d治疗组总有效率显著高于对照组(94.82% vs 82.76%,P<0.05);治疗后60d治疗组复发例数明显少于对照组(12.07% vs 29.31%,P<0.05).结论 定君生联合两性霉素B对妊娠期外阴阴道假丝酵母菌病的临床疗效显著,且其治疗效果维持时间长,可较好的预防患者复发.

  13. 类风湿性关节炎继发念珠菌性外阴阴道炎76例%CLINICAL ANALYSIS OF 76 CASES OF VULVOVAGINAL CANDIDIASIS SECONDARY TO RHEUMATOID ARTHRITIS

    陈维飞

    2011-01-01

    [Objective] To analysis the incidence, symptom, predisposing factors and therapeutic results of vulvovaginal candidiasis secondary to rheumatoid arthritis. [Methods] Local and systemic antifungal treatment, immunotherapy were used in the treatment of 76 cases of vulvovaginal candidiasis secondary to rheumatoid arthritis. [Results] 47 (61.8%) patients were cured, 5 (6.6%) patients responded to the therapy, 3 patients failed to respond to therapy, side effects were found in 5 patients. [Conclusion] The incidence of vulvovaginal candidiasis is high in patients with rheumatoid arthritis, treatment outcome was satisfactory in most patients.%[目的]研究分析类风湿性关节炎继发念珠茵性外阴阴道炎的发病情况、临床症状、易感因素及治疗中注意事项.[方法]采用局部及全身应用抗真茵用药、增强免疫力等方法治疗.[结果]经积极治疗后痊愈47例(其中初发22例,复发25例),痊愈率61.8%,显效21例(其中初发9例,复发12例),显效率27.6%,有效5例(其中初发1例,复发4例),有效率6.6%,无效3例(其中初发O例,复发4例),总有效率96%.不良反应5例,症状可以控制.[结论]类风湿性关节炎合并念珠茵性外阴阴道炎的发病率明显高于正常人群,且易复发,由于肌体免疫力下降,治疗过程中要注意避免复发的一些因素,总体疗效满意.

  14. 中西医结合治疗外阴阴道假丝酵母菌病临床研究%Clinical Study on Vulvovaginal Candidiasis by Intertrated Traditioal Chinese and Western Medicine

    高玉林

    2013-01-01

    目的:探讨中西医结合治疗外阴阴道假丝酵母茵病(VVC)的临床疗效.方法:将280例VVC患者按随机数字表法分为两组,对照组140例用盐酸特比奈芬阴道泡腾片阴道用药;观察组140例在对照组基础上配合中药水剂灌洗外阴及阴道.两组均以7d为1个疗程,连续2个疗程.结果:观察组治愈率为94.3%,有效率为98.6%;对照组治愈率为73.6%,有效率为85.0%.观察组复发率为3.6%,对照组复发率为12.8%.两组临床疗效、复发率比较有显著性差异(P<0.05).结论:中西医结合治疗RVVC疗效显著、复发率低.%Objective:To observe the clinical effect of vulvovaginal candidiasis(VVC) by intertrated traditioal Chinese and western medicine.Methods:280 cases of vulvovaginal candidiasis were randomly divided into two groups.The control group(140 cases) was treated with terbinafine hydrochloride vaginal effervescent tablets.While the observation group(140 cases) was treated with traditional Chinese medicine water irrigation vulva and vagina based on the control group.The course of treatment was one month in the two groups and continuously two courses.Results:The cure rate was 94.3%,effective rate was 98.6% in the observation group ;The cure rate was 73.6%,effective rate was 85.0% in the control group.The recurrent rate was 3.6% in the observation group,while in the control group was 12.8%.Comparison of the effective,recurrent rates in two groups showed that the difference was significant difference(P < 0.05).Conclusion:The combination of traditional Chinese and western medicine in the treatment of vulvovaginal candidiasis has a better curative effect and a lower recurrent rate.

  15. An unusual case: Renal candidiasis; Der besondere Fall: Renale Candidiasis

    Pautz, Doerthe [Medizinische Hochschule Hannover (Germany). Inst. fuer Radiologie

    2009-03-15

    A 39-year old female patient suffering from congenital spastic tetraparesis, bilateral hip dysplasia and diabetes was hospitalized as an emergency because of violent pains in the right flank which emanated paravertebrally and into the pelvis. There was no fever or ague. The anamnesis included urolithiasis on the right side 5 years ago and an ileum conduit operation after a neurogenic disturbance of micturition 22 years ago. The sonographic pictures indicated no congestion of the kidneys. To exclude urolithiasis, a low-dose CT was carried out, followed by CT after application of a contrast medium. (orig.)

  16. Thrush (Oral Candidiasis) in Children

    ... Self-Care Guidelines Thrush may make eating and drinking uncomfortable, and children with thrush may lose water in their body tissues, becoming dehydrated. It is important to make sure your child maintains good nutrition and fluid intake (hydration) while infected with thrush. See your child's ...

  17. Candidiasis oral en paciente mayor

    Somacarrera Pérez, María Luisa

    2012-01-01

    La población octogenaria española se ha disparado en nuestro país aumentando en más de un 20% en los últimos cinco años, mientras que, en términos generales, los españoles de más de 65 años se han incrementado en un 5%.  España, con 7,5 millones de mayores (16% de la población) se coloca en el cuarto lugar de los países más envejecidos del planeta, sólo superada por Japón, que encabeza la lista con un 20% de población mayor, Italia y Alemania. Este envejecimiento conlleva un aumento de la pat...

  18. Aphthoid ulcerations in ileocolic candidiasis

    A case of well differentiated lymphocytic lymphoma complicated by Candida tropicalis ileocolic infection is reported. Candida tropicalis is a saprophytic organism, less abundant in man than the more commonly known Candida albicans. These organisms may be the source of infection in debilitated or immune-deficient patients. A 55-year-old man presented to Emory Hospital 7 months before admission with weight loss. At that time the diagnosis of well differentiated lymphocytic lymphoma was made by bone-marrow biopsy

  19. Spinal cord swelling and candidiasis

    Fusiform swelling of the spinal cord was noted myelographically in a patient with Hodgkin's disease. Autopsy revealed that the swelling was cauused by Candida infection of the spinal cord. It is suggested that fungal infection be included in the differential diagnosis of spinal cord swelling in the immunsupporessed cancer patient. (orig.)

  20. Spinal cord swelling and candidiasis

    Ho, K.; Gronseth, G.; Aldrich, M.; Williams, A.

    1982-11-01

    Fusiform swelling of the spinal cord was noted myelographically in a patient with Hodgkin's disease. Autopsy revealed that the swelling was caused by Candida infection of the spinal cord. It is suggested that fungal infection be included in the differential diagnosis of spinal cord swelling in the immunosuppressed cancer patient.

  1. Aphthoid ulcerations in ileocolic candidiasis

    Gedgaudas-McClees, R.K.

    1983-11-01

    A case of well differentiated lymphocytic lymphoma complicated by Candida tropicalis ileocolic infection is reported. Candida tropicalis is a saprophytic organism, less abundant in man than the more commonly known Candida albicans. These organisms may be the source of infection in debilitated or immune-deficient patients. A 55-year-old man presented to Emory Hospital 7 months before admission with weight loss. At that time the diagnosis of well differentiated lymphocytic lymphoma was made by bone-marrow biopsy.

  2. An unusual case: Renal candidiasis

    A 39-year old female patient suffering from congenital spastic tetraparesis, bilateral hip dysplasia and diabetes was hospitalized as an emergency because of violent pains in the right flank which emanated paravertebrally and into the pelvis. There was no fever or ague. The anamnesis included urolithiasis on the right side 5 years ago and an ileum conduit operation after a neurogenic disturbance of micturition 22 years ago. The sonographic pictures indicated no congestion of the kidneys. To exclude urolithiasis, a low-dose CT was carried out, followed by CT after application of a contrast medium. (orig.)

  3. Pulmonary candidiasis presenting as mycetoma

    Bachh Arshad

    2008-01-01

    Full Text Available Candida is a saprophytic yeast that is frequently recovered from the respiratory tract. Most mycetoma lesions are due to Aspergillus species growing inside an existing cavity. The saprophytic nature of the Candida species in the human respiratory tract obscures diagnosis of Candida pulmonary infections. Only a few cases of mycetoma due to Can-dida have been reported. We report a case of mycetoma caused by Candida albicans in a diabetic immunocompromised tuberculous patient. Diagnosis was confirmed by biopsy and certain points strongly favoured the diagnosis. The patient was immunocompromised due to uncontrolled diabetes mellitus. Candida albicans was grown from bronchial and repeatedly from sputum samples and Candida antigen was positive from blood in high titre. There was a good clinical as well as radiological response to antifungal therapy and Candida antigen became negative. We emphasize that in the immunosuppressed host, a mycetoma-like lesion may be caused by Candida pulmonary infection.

  4. 不孕妇女阴道念珠菌病致病菌分析%Analysis of pathogenic fungi causing vulvovaginal candidiasis in infertility women

    唐晓华; 江镜全; 叶俊凯; 蔡媛媛; 夏勇

    2014-01-01

    Objective To investigate the distribution and drug resistance of pathogenic fungi causing vulvovaginal candidiasis(VVC)in infertility women,and provide the reference for clinical treatment.Methods The CHROMagar Candida color medium was applied to identify Candida isolated from the vaginal secretions collected from infertility women,and Rosco disc diffusion method was used for drug susceptibility.Results A total of 86 Candida isolates were isolated from 454 vaginal secretion samples,including 48 isolates of Candida albicans(55.81%),24 isolates of Candida glabrata(27.91%),8 isolates of Candida tropicalis (9.30%),4 isolates of Candida krusei (4.65%)and 2 isolates of Candida parapsilosis (2.33%).The sensitivity rates of Candida isolates to amphotericin B,ketoconazole,miconazole, fluconazole and itraconazole were 93.55%,77.91%,65.1 2%,62.79% and 59.30% respectively.Conclusions The non-Candida albicans infection in infertility women with VVC increases obviously,and the drug sensitivities of different types of Candida isolates are different.Therefore,antibiotic drugs should be carefully used being based on the results of fungal culture identification and drug sensitivity test.%目的:了解本地区不孕妇女外阴阴道念珠菌病(VVC)致病菌的菌种分布及药物敏感性情况,为临床治疗提供参考。方法采集不孕妇女阴道分泌物样本,留取念珠菌阳性样本,采用科玛嘉念珠菌显色培养基做培养鉴定,并采用Rosco纸片扩散法做药物敏感性试验。结果收集的454例样本中共分离出86株念珠菌,其中白念珠菌48株,占55.81%;光滑念珠菌24株,占27.91%;热带念珠菌8株,占9.30%;克柔念珠菌4株,占4.65%;近平滑念珠菌2株,占2.33%。分离的菌株对两性霉素B、克霉唑、咪康唑、氟康唑、伊曲康唑的敏感率分别为93.55%、77.91%、65.12%、62.79%、59.30%。结论非白念珠菌在不孕妇女VVC中分

  5. 臭氧液阴道冲洗治疗假丝酵母菌病的疗效观察%Efficacy of douching vagina with ozone liquid in treatment of vulvovaginal candidiasis

    罗翠华; 陈晓燕; 李伟妙; 罗建华

    2014-01-01

    目的:观察单纯臭氧灭菌液配合改良阴道冲洗和臭氧液直接阴道内冲洗配合阴道给药两种不同方法的临床疗效,为进一步改进假丝酵母菌病的治疗方法提供依据。方法将2008年6月-2012年10月200例确诊外阴阴道假丝酵母菌病的患者随机分成两组,观察组100例采用单纯臭氧灭菌液配合改良阴道冲洗治疗,每天1次,疗程3 d;对照组100例采用臭氧液直接阴道内冲洗配合达克宁栓阴道给药,每天1次,疗程3 d ,每次冲洗前进行临床、真菌学疗效分析,比较两组的疗效及不良反应。结果治疗3 d后,观察组痊愈率达到91.0%,总有效率达98.0%;对照组痊愈率达57.0%,总有效率达81.0%;观察组症状缓解时间明显短于对照组,两组比较差异有统计学意义(P<0.05)。结论单纯臭氧灭菌液配合改良阴道冲洗治疗外阴阴道假丝酵母菌病疗效好,并且操作简单安全、不良反应少,患者容易接受,值得在临床中推广使用。%OBJECTIVE To observe the clinical curative effect of the treatment of vulvovaginal candidiasis by using pure ozone sterilizing liquid with modified vaginal douche compared with the method of douching the vagina with ozone liquid and vaginal drug delivery ,so as to providing evidence to modify the treatment of vulvovaginal candidi-asis further .METHODS Totally 200 cases of patients with vulvovaginal candidiasis in Jun 2008 to Oct 2012 were randomly divided into two groups .The 100 patients of observation group were treated with pure ozone sterilizing liquid with modified vaginal douche ,1 d ,period of treatment 3 d .The 100 patients of control group was treated by douching the vagina with ozone liquid directly and applying miconazole suppository ,1 d ,period of treatment 3 d .Each time the clinical and mycological efficacy were analyzed before treatment and the efficacy and adverse reac-tions were compared between

  6. Relevance between acidic environment of vagina and vulvovaginal Candidiasis%阴道内酸性环境对白色念珠菌性外阴阴道炎治疗的关联研究

    施磊; 宗黎琼; 童剑倩; 郭澄

    2012-01-01

    Objective To explore the relevance between the acidic environment of vagina and vulvovaginal Candidiasis infected by Candida albicans. Methods Ninety-six strains from patients (June 2009 to March 2010) with Simple Gynecological Candida Vulvovaginitis (SVVC) were isolated and identified. The MIC of miconazole and clotrimazole under different pH value of environment were measured. Results The MIC of miconazole or clotrimazole was decreased under lower pH value. Conclusion Keep the acidic environment of vagina is beneficial to the treatment of vulvovaginal Candidiasis infected by Candida albicans.%目的 探讨阴道内酸性环境与白色念珠菌性外阴阴道炎的相关性.方法 分别对2009年6月至2010年3月妇科门诊的单纯性外阴阴道假丝酵母菌病(SVVC)患者进行阴道菌株分离、鉴定,获得白色念珠菌96株.检测不同pH值环境下菌株对硝酸咪康唑与克霉唑的药物敏感性,并观察相关性.结果 在pH值偏酸性的环境下,两种唑类药物的MIC均有不同程度的降低,对白色念珠菌抑菌效果更强.结论 维持阴道内酸性环境能更有效地治疗白色念珠菌性外阴阴道炎.

  7. 外阴阴道假丝酵母菌病过度医疗现况调查%A cross-sectional investigation on excess medical treatment of vulvovaginal candidiasis

    肖敏; 梁秀; 郭爱华; 李汉金; 胡序怀

    2011-01-01

    Objective: To understand the status of over - diagnosis and over - treatment of vulvovaginal candidiasis in medical institutions, provide a basis for making standardizing and regulatory diagnosis and treatment criterions of vulvovaginal candidiasis, in order to reduce the cost and save medical sources.Methods: 93 doctors who were working in the obstetrics and gynecology department, skin and venerology section, family planning department and clinical laboratory of the general hospitals, specialized hospitals, family planning services centers and private hospitals were surveyed by anonymous questionnaires, the actual diagnosis and treatment of vulvovaginal candidiasis were understood, then the results were compared with chnical operating instructions.Results: The average age of the investigated doctors was (38.6 ±9.3) years old, from 22 years to 61 years; the average medical work time was 16.3 years; educational levels: the proportions of college and university, master or higher, other educational levels were 62.4%, 23.7% and 13.9%, respectively; among 39 laboratory doctors, 29 doctors had over - diagnosis, accounting for 74.4%; among the clinical doctors, 35.2% of them selected laboratory diagnosis as standard, 77.8% of them had over - diagnosis; 77.8% of the clinical doctors had over - treatment; during the course of treatment of vulvovaginal candidiasis, the treatment was not standard; asymptomatic vulvovaginal candidiasis affected the process of family planning operations.Conclusion: The status of excess medical treatment in clinical doctors and medical laboratory doctors are severe, which affect the process of family planning operations; thus, the standardized management of clinical work of clinical diagnosis and treatment should be enhanced to save medical sources.%目的:了解医疗机构外阴阴道假丝酵母菌病过度诊断和过度治疗的现状,为制定外阴阴道假丝酵母菌病标准化、规范化诊断治疗规范提供参考

  8. 乳酶生片联合达克宁栓治疗复发性念珠菌性阴道炎疗效观察%Lactasin tablet combined with miconazole suppository for recurrent vulvovaginal candidiasis

    吕金霞

    2012-01-01

    目的 观察乳酶生片联合达克宁栓治疗复发性念珠菌性阴道炎疗效.方法 300例复发性念珠菌性阴道炎患者随机、单盲分为治疗组150例和对照组150例,对照组采用达克宁栓200 mg,阴道上药,每晚一次,共用7天.治疗组在对照组的基础上加用乳酶生片0.6 g,每晚一次,共用7~10天.两组用药均每月重复一次,连用3个月.分别于治疗前,治疗后1个月、2个月、3个月进行疗效观察,比较复发情况.结果 治疗组总有效率为94.7%,治疗3个月后复发率为5.97%;对照组总有效率为85.3%,治疗3个月后复发率为19.04%,治疗组疗效明显优于对照组(P<0.01),治疗组各症状复发明显少于对照组(P<0.05)且治疗组复发率明显低于对照组(P<0.0l).结论 乳酶生片与达克宁栓合用治疗复发性念珠菌性阴道炎效果良好,可明显减少复发.%Objective To observe lactasin tablet combined with miconazole suppository in the treatment of recurrent vulvovaginal candidiasis.Methods 300 patients with recnrrent vulvovaginal candidiasis were randomly and blindly divided into study group and control group,150 for each group.The control group received miconazole vaginal suppository of 200 mg once per night for 7 to 10 days,while the study group received lactasin tablet of 600 mg per night for 7 to 10 days in addition to therapy with vaginal suppository.The same therapy was applied twice monthly in the same groupfor three months.The efficacies were observed in both groups one,two,and three months after treatment; the recurrence rates were compared.Results The study group was superior to the control group in the efficacy ( 94.7% vs.85.3% for the total effectiveness rate,5.97% vs.19.04% for the recurrence rate 3 months after treatment; P< 0.01 ).The rate of symptom recurrence was significantly lower in the study group than in the control group ( P < 0.05 ),and so was the recurrence rate ( P < 0.01 ).Conclusions

  9. 洁阴洗剂治疗复发性念珠菌性外阴阴道炎的疗效观察%The Efficacy of Jieyin Lotion in the Treatment of Recurrent Vulvovaginal Candidiasis

    陈红

    2014-01-01

    目的:探讨洁阴洗剂治疗复发性念珠菌性外阴阴道炎的疗效及应用价值。方法:选择笔者所在医院收治的160例复发性念珠菌性外阴阴道炎患者作为研究对象,随机分为对照组和观察组,对照组采用口服氟康唑进行治疗,观察组在对照组治疗的基础上联合使用洁阴洗剂进行治疗,观察两组临床治疗效果。结果:观察组总有效率为76.25%,对照组总有效率为57.50%,两组比较差异有统计学意义(P<0.05)。观察组治疗后1、2、3个月复发率均少于对照组,差异有统计学意义(P<0.05)。结论:采用洁阴洗剂联合口服氟康唑治疗复发性念珠菌性外阴阴道炎疗效优于单纯口服氟康唑的治疗,复发率较低,值得在临床上大力推广使用。%Objective:To investigate the efficacy and application value of Jieyin lotion in the treatment of recurrent vulvovaginal candidiasis. Method:160 cases patients in author’s hospital with recurrent vulvovaginal candidiasis for the study were randomly divided into control group and observation group,the control group were treated with oral fluconazole,the observation group were treated with Jieyin lotion treatment on the basis of the control group treatment method,the clinical effect of two groups were observed.Result:The total effective rate in the observation group was 76.25%,the total effective rate in the control group was 57.50%,the two groups by statistical analysis,the difference was statistically significant(P<0.05).After treatment 1,2,3 month,the recurrence rate in the observation group were less than the control group,the difference was statistically significant(P<0.05).Conclusion:The effective of Jieyin lotion combined with oral fluconazole treatment of recurrent vulvovaginal candidiasis is better than oral fluconazole treatment,the recurrence rate is low,it is used in clinical practice to promote.

  10. The efficacy and safety of terconazole suppositories for treatment of vulvovaginal candidiasis%特康唑阴道栓治疗外阴阴道假丝酵母菌病多中心临床研究

    汪清; 胡烈薇; 滕宗荣; 王敏敏; 郑瑞莲; 曹斌融; 狄文; 张惠英; 段涛; 黄冰清; 王玲; 戴晓星

    2012-01-01

    目的 评价特康唑阴道栓治疗外阴阴道假丝酵母菌病( vulvovaginal candidiasis,VVC)的有效性和安全性.方法 采用多中心、随机、双盲对照的研究方法,将2004年5月至8月在复旦大学附属妇产科医院等4家医院就诊并确诊的139例外阴阴道假丝酵母菌病患者随机分为治疗组(特康唑组70例)和对照组(硝酸咪康唑组69例).连续用药7d,分别于给药后1周和4周进行临床疗效、微生物疗效和复发率比较.结果 两组VVC患者治疗后症状和体征均得到有效控制,但治疗组在给药4周后的临床症状和微生物学检查的复发率均为0,对照组的复发率分别为10.17%、16.67%,差异有统计学意义(P<0.05).两组的不良反应发生率分别为5.7%和5.8%,差异无统计学意义(P>0.05),均未见严重不良事件.结论 特康唑阴道栓抗真菌谱广、复发率低、安全性好,可作为治疗VVC的一个新选择.%Objective To evaluate the efficacy and safety of terconazole suppositories for treatment of Vulvovaginal Candidiasis. Methods 139 valid case were randomly assigned into observation group or control group for 7-day treatment in this randomized multi-center double blind study. During the trial, the follow-up visits were conducted at 1 and 4 weeks after treatment respectively. Clinic cure rates, microbiologic cure rates and relapse rates were observed in each follow-up visit. Results Both groups had significantly therapeutic cure rates. Clinic and microbiologic relapse rates for terconazole regimens, however, were 0 and control group were 10. 17% and 16.67% Respectively. Conclusion Terconazole suppositories is a preferable candidate for treatment of Vulvovaginal Candidiasis.

  11. Candidíase sistêmica com localização encefálica: estudo anátomo-clínico de cinco casos Septicaemia candidiasis with cerebral involvement: a report of five cases

    Luciano de Souza Queiroz

    1976-03-01

    Full Text Available Nas septicemias por Candida, a localização encefálica é rara, e apenas quatro casos isolados foram publicados no Brasil. Os AA. apresentam cinco observações anátomo-clínicas de candidíase do sistema nervoso central, diagnosticadas somente pela autópsia. Quatro dos pacientes eram adultos e apenas uma criança foi estudada. Todos eram portadores de uma ou mais doenças de base e foram submetidos à terapêutica antibiótica múltipla e prolongada. Os sintomas neurológicos eventualmente atribuíveis à infecção fúngica limitaram-se a convulsões em um caso e rigidez de nuca em outro. Em nenhum as lesões assumiram gravidade suficiente para serem responsabilizadas como causa imediata da morte. Revelaram-se múltiplas e microscópicas em três pacientes, com caráter exsudativo e granulomatoso; macroscópicas em um caso, com aspecto necro-hemorrágico. Na criança, dois granulomas apenas foram observados. O agente etiológico foi identificado como Candida, nos cortes histológicos, pela técnica de impregnação argêntica de Grocott. Os autores discutem a patogênese e a anatomia patológica da candidíase sistêmica, bem como seu diagnóstico clínico-laboratorial e a terapêutica. Os achados clínicos e an átomo-patológicos dos presentes casos são analisados à luz da literatura, enfatizando que a incidência de monilíase sistêmica no Brasil deve ser muito superior ao sugerido pela escassa casuística nacional.Central nervous system involvement in Candida septicaemia is rare and not more than four cases have been published in Brazil. Five new cases of systemic candidiasis with cerebral lesions are reported. All patients (four adults and a child had serious underlying diseases and were submitted to heavy long-term antibiotic therapy with multiple drugs. Seizures in one case and neck stiffness in another were the only neurologic signs that could be attributed to candidiasis. In no case were the lesions severe enough to be

  12. Clinical observation of sertaconazole nitrate cream in treatment of skin candidiasis in children%硝酸舍他康唑乳膏治疗儿童皮肤念珠菌病50例

    吴启富

    2012-01-01

    目的 观察硝酸舍他康唑乳膏治疗儿童皮肤念珠菌病的疗效和安全性.方法 将就诊的100例皮肤念珠菌病患儿分为两组,治疗组50例,外搽硝酸舍他康唑乳膏2次/d,对照组50例,外搽硝酸咪康唑软膏2次/d,疗程7~14d.观察其临床疗效和不良反应.结果 治疗组临床痊愈率92.00%,对照组64.00%,两组比较差异有统计学意义(P<O.01).与对照组比较,治疗组起效更快、皮疹消失时间更短(P<0.01).不良反应少而轻.结论 硝酸舍他康唑乳膏治疗儿童皮肤念珠菌病起效快,安全有效,具有较高的临床应用价值.%Objective To evaluate clinical efficacy and safety of sertaconazole nitrate cream in the treatment of children's skin candidiasis (moniliasis). Methods One hundred patients were enrolled in the test and were divided into 2 groups. Sertaconazole nitrate cream was applied topically to the affected areas of the body twice daily for 14 days in the treatment group. Miconazole nitrate ointment was applied topically to the affected areas of the body twice daily for 14 days in the control group. The efficacy and the adverse reaction were observed. Result Treatment groups curative rate is 92. 00% and control group's 64. 00% ( P < 0. 01). Compared with the control group, the treatment group produced the effect more quickly with a shorter cured period ( P <0. 01). There was little adverse effect observed in the test. Conclusion Sertaconazole nitrate cream acts effectively and safely on children's skin candidiasis. It is worthy of application.

  13. Clinical Analysis of Antifungal Agents in the Treatment of Vulvovaginal Candidiasis Susceptibility%抗真菌药物治疗外阴阴道念珠菌病敏感性的临床分析

    万丽琼

    2014-01-01

    Objective To explore the sensitivity of the antifungal treatment of vulvovaginal candidiasis. Methods To make 538 patients with the vulvovaginal candidiasis in our hospital between 2010 and 2012 as research subjects, analyze their clinical date retrospectively, and to observe and analyze the sensitivity of the antifungal to the disease. Results After the separation of candida from the 538 patients, the candida albicans accounted for 61.9%(333/538), and the non-albicans accounted for 38.1%(205/538). There were significant dif erences ( 0.05) and statistical y significant between the sensitivity of Ketoconazole and Amphotericin B to both of them. Conclusion For VVC, the main pathogen was candida albicans, and the candida albicans drug sensitivity was higher than that of non-albicans, but its antifungal drug resistance was rare.%目的探讨抗真菌药物治疗外阴阴道念珠菌病(VVC)敏感性。方法将我院2010年~2012年收治的538例外阴阴道念珠菌病患者作为研究对象,对他们临床资料进行回顾性分析,同时对抗真菌药物治疗该病的敏感性进行观察分析。结果对538株念珠菌进行分离后可知,其中白念珠菌占了61.90%(333/538),非白念珠菌则占了38.10%(205/538)。白念珠菌及非白念珠菌,在氟康唑与伊曲康唑的敏感度上有着显著性差异(0.05),没有统计学意义。结论对于VVC而言,其主要的致病菌当属白念珠菌,同时白念珠菌的药物敏感性要比非白念珠菌要高,但其对抗真菌药物的耐药性却不多见。

  14. 硝酸舍他康唑栓治疗外阴阴道假丝酵母菌病28例%28 Cases of Vulvovaginal Candidiasis Treated with Sertaconazole Nitrate Suppositories

    董迪荣; 周春; 张元珍; 马玲; 郭清莲

    2011-01-01

    目的 评价硝酸舍他康唑栓治疗外阴阴道假丝酵母菌病的疗效及安全性.方法 采用随机双盲对照试验.治疗组及对照组各30例,分别外用硝酸舍他康唑栓300 mg和克霉唑栓500 mg,用药1或2次.停药后(7±1) d及28~35 d时进行观察.结果 治疗组28例、对照组30例纳入分析,治疗组和对照组停药后(7±1) d临床有效率分别为89.29%,86.67%(P>0.05);用药结束后28~35 d时的临床有效率分别为81.48%,66.67%(P>0.05);均未发生与药物相关的不良反应.结论 硝酸舍他康唑栓治疗外阴阴道假丝酵母菌病有效,安全.%Objective To evaluate the efficacy and safety of sertaconazole nitrate suppositories for treating vulvovaginal candidiasis Methods A double-blind, randomized controlled trial was conducted 30 cases in both treatment group and control one were applied with sertaconazole nitrate suppositories and clotrimazole ones for1-2 times respectively Patients were evaluated at day 7±1 and day 28-35 after ceasing medication Results 28 cases in the medical trial group and 30 cases in control were evaluated; The clinical efficacy at day7±1 were 89.29% in the test group and 86.67% in control group; and 81.48% versus 66. 67% at day 28-35 after drug withdrawn, respectively No treament related adverse events occurred in both groups Conclusion This study suggests that sertaconazole nitrate suppositories are effective and safe for the treatment of vulvovaginal candidiasis

  15. 复发性外阴阴道假丝酵母菌病治疗方案及疗程的研究分析%Analysis of Recurrent Vulvovaginal Candidiasis in Therapeutic Regimen and Course of Treatment

    李丽平

    2015-01-01

    Objective To investigate therapeutic regimen and course of recurrent vulvovaginal candidiasis. Methods From 2012 September to 2013 June, 80 cases with recurrent vulvovaginal candidiasis were treated in Lingyuan Central Hospital,they were randomly divided into 2 groups.control group ( 40 cases ) received fluconazole + clotrimazole regimen as initial treatment,and lasted for seven to 10 days,clotrimazole was used maintenance therapy for 6 months;initial treatment of the observation group(40 cases) was the same as the control group,maintenance treatment used clotrimazole plus lactasin regimen for 3 months.Results The two groups of patients in the clinical cure rate, there was no significant difference between the recurrence rate (P>0.05),but in the course of time the observation group was better than the control group, there was significant difference (P<0.05).Conclusions In the observation group and the control group,the two treatment methods are very effective, but the treatment time in observation group than the con-trol group, worthy of promotion.%目的:探讨研究复发性外阴阴道假丝酵母菌病的治疗方案及疗程。方法将2012年9月~2013年6月收治80例复发性外阴阴道假丝酵母菌病患者按着随机分为两组。对照组40例初始治疗采取氟康唑+克霉唑治疗7天,维持治疗采取克霉唑治疗6个月;观察组40例初始治疗同对照组,维持治疗在应用克霉唑的同时加乳酶生治疗3个月。结果两组患者在临床治愈率,复发率方面比较无统计学意义( P>0.05),但在疗程时限上观察组明显优于对照组,有显著差异( P<0.05)。结论观察组和对照组所采取的两种治疗方法都很有效,但在治疗时限上观察组优于对照组,值得推广。

  16. 范瑞强教授治疗复发性外阴阴道念珠菌病经验%Professor Fan Ruiqiangs Experience of Treating Vulvovaginal Candidiasis

    王平; 樊志奇; 范瑞强

    2014-01-01

    复发性外阴阴道念珠菌病(Recurrent Vulvo Vaginal Candidiasis,RVVC)是一种常见的由念珠菌感染引起的女性外阴皮肤黏膜真菌病。范瑞强教授认为该病的病机主要是湿热蕴结,湿热生虫,日久肝脾不和,脾虚肝郁,治疗方面,范教授认为本病的治疗应以外治为主,内外兼顾,内治则应辨证论治。%Recurrent Vulvo Vaginal Candidiasis(RVVC)is a type of common mycoses in the vagina which is caused by Monilia.In Pro-fessor Fan Ruiqiangs opinion,the pathogenesis of RVVC is the accumulation of damp heat giving birth to worms,as time goes by,liver-spleen becomes disordered,with spleen being deficient and liver being stagnant.When it confers to treatment of RVVC,professor Fan considers that external treatment is more important than internal treatment,and syndrome differentiation should be applied.

  17. An Evaluation of Theraphy with Fluconazole 150 mg Tablets Compared to Fluconazole 150 mg Tablets Plus Dermoxen Lenitiva Cream in The Time to Reduce Simptomatology in Women with Vulvovaginal Candidiasis

    Davide Carati

    2014-06-01

    Full Text Available Aim of the study. Authors investigated first of all the time to onset of first relief of symptoms. Secondary measures included the time to overall relief of symptoms and the reoccurrence rate over the first 45 days after the first visit. Methods. A randomized, open-label, parallel study evaluated 47 women with moderate to severe symptoms of Vulvo Vaginal Candidiasis (VVC. Patients were divided into two groups of treatment: group 1 followed a therapy with Fluconazole 150 mg tablets, while group 2 had a therapy based on Fluconazole 150 mg tablets coadjuvated by Dermoxen Lenitiva cream. Results. The time at which 50% of patients experienced first relief of symptoms was 24.6 hours for Group 1, while for Group 2 it was 12.4 hours (P<0.05. There were significant differences between the two groups in respect to the time of first relief of symptoms and reoccurrence of infection within 45 days of treatment. Conclusions. Combined treatment with Fluconazole 150 mg tablets and by Dermoxen Lenitiva cream provides statistically significant improvement in the time of first relief of symptoms, complete relief of symptoms and relapse time in the treatment of VVC compared to fluconazole 150 mg tablets only.

  18. 妊娠期外阴阴道假丝酵母菌病治疗方法初探%Original study of the treatment of vulvovaginal candidiasis during prengnancy

    杨云; 金桂红

    2011-01-01

    外阴阴道假丝酵母菌病(vulvovaginal candidiasis,vvC)是由假丝酵母菌引起常见的外阴阴道炎症[1],是一种念珠菌(candidaalbicans)过度生长导致的外阴阴道感染.20%~30%孕妇阴道内存在酵母菌,妊娠期VVC是孕妇常见的合并症之一,对母子均可以造成伤害,可引起胎膜早破、早产、宫内感染,引起新生儿感染(尿布皮炎、鹅口疮).凯妮汀是一种广谱抗真菌制剂,主要成份为克霉唑,是咪唑衍生物,抑制真菌细胞的重要成份麦角甾醇的生物合成.该药已广泛用于非妊娠妇女患霉菌性阴道炎的治疗.本研究旨在探讨克霉唑阴道片治疗妊娠期VVC的有效性.

  19. Observation of the Curative Effect of Clotrimazole Combined With Fluconazole in the Treatment of Recurrent Vulvovaginal Candidiasis%克霉唑联合氟康唑治疗复发性外阴阴道假丝酵母菌病的疗效观察

    朱屹峰; 陈丽红

    2015-01-01

    Objective To explore the efifcacy of clotrimazole combined with lfuconazole in the treatment of recurrent vulvovaginal candidiasis. Methods 85 patients with recurrent vulvovaginal candidiasis were randomly divided into control group (40 cases) and treatment group (45 cases), the control group was taken clotrimazole alone, the treatment group was taken clotrimazole combined with fluconazole therapy. Results The total efifciency of the patients in the treatment group were higher than that of control group (P recurrent vulvovaginal candidiasis.%目的:探究克霉唑联合氟康唑治疗复发性外阴阴道假丝酵母菌病的临床疗效。方法选取我院复发性外阴阴道假丝酵母菌病患者85例,随机分为对照组40例和治疗组45例,对照组单独使用克霉唑治疗,治疗组采用克霉唑联合氟康唑治疗。结果治疗组患者治疗总有效率高于对照组(P<0.05),不良反应发生率低于对照组(P<0.05),差异有统计学意义。结论采用克霉唑联合氟康唑治疗复发性外阴阴道假丝酵母菌病,患者治疗总有效率高,不良反应少,临床疗效显著。

  20. Check with square mainly joint dink treatment vulvovaginal candidiasis clinical research%止带方为主联合丁克治疗外阴阴道假丝酵母菌病的临床研究

    刘婷; 谢波

    2011-01-01

    目的 观察止带方为主联合丁克阴道泡腾片治疗外阴阴道假丝酵母菌病的临床效果.方法 选取我院自2007年1月至2009年12月收治的外阴阴道假丝酵母菌病患者136例随机分为观察组(中西医联合治疗组)和对照组(达克宁栓治疗组)各68例,治疗周期均为4周,比较两组患者的临床治疗效果.结果 (1)观察组治愈45例,显效17例,总有效率为91.2%;对照组治愈28例,显效19例,总有效率为69.1%.两组比较差异有统计学意义(P<0.01),具有统计学意义.(2)观察组出现外阴灼痛加重者1例,恶心者1例;时照组出现外阴灼痛加重者2例.两组比较无统计学意义(P>0.05).结论 采用止带方为主联合丁克阴道泡腾片治疗外阴阴道假丝酵母菌病疗效理想,值得临床推广使用.%Objective To observe only the main parties combined with the treatment of vulvovaginal Dink Effervescent Tablets Candida clinical effects of the disease. Methods Select our hospital from January 2007 ~December 2009 admitted patients with vulvovaginal candidiasis were randomly divided into two groups 136 patients (combined group Chinese and Western) and the control group (Miconazole suppository in the treatment group) 68 cases, the treatment period was 4 weeks, compareed two groups of clinical treatment. Results (1) the observation group were cured in 45 cases,markedly effective in 17 cases,totel effective rate was 91.2%;control group, 28 cases were cured, effective in 19 cases,total effective rate was 69.1%.Significant difference between the two groups (P<0.01), with statistical significance.(2) increase the observation group occurred in 1 case vulvar burning, nausea in 1 case; the control group there were two cases of vulvar burning increased. No significant difference between the two groups (P>0.05).ColClUsion The party ended with Dink vagina effervescent tablets of combination treatment vulvovaginal candidiasis efficacy ideal, worthy of clinical use.

  1. Observation of 300 cases for severe vulvovaginal candidiasis treated with three regimen%3种方案治疗重度外阴阴道念珠菌病300例疗效观察

    冯兰凤

    2011-01-01

    Objective To observe and evaluate the curative effects of three regimens for severe vulvovaginal candidiasis (VVC). Methods 300 patents with VVC were divided into three groups. Group A; 100 women, were scrubbed their walls of the vagina with the iodophor solution (0. 5 g/L) and sprayed with the nysfungin powder in hospital for 7 consecutive days. Group B: 100 women, Oral Fluconazole ISOrag quaque day, for the first day and third day. Group C; 100 women, Oral Fluconazole 150 mg quaque day for the first day, and were scrubbed their walls of the vagina with the iodophor solution and sprayed with the nysfungin powder in hospital for 7 consecutive days. Satisfaction, efficacy, and microscopic examination of fungal were observed. Results There was no significant difference between Group A and Group C on satisfaction 1, 3 and 6 days after treatment ( P >0.05). Satisfaction in Group A and Group C was higher than that in Group B ( P 0.05 ). The effective rate and long term effect in Group A and Group C were higher than that in Group B ( P <0.05). Conclusion Three projects for VVC were all effective. The improvement of symptoms in group with vaginal medication is superior to those with oral medication. The long - term curative effectiveness of vaginal medication combined with oral medication is better than that of vaginal medication alone.%目的 观察3种方案治疗重度外阴阴道念珠茵病(vulvovaginal candidiasis,VVC)的临床疗效.方法 将300例重度VVC患者随机分为3组,制霉素粉组100例予0.5%碘伏液擦拭阴道外阴后,阴道外阴喷洒制霉素粉,连用7d;氟康唑组予氟康唑150 mg,每日1次口服;联合用药组予氟康唑150 mg,每日1次口服,同时予0.5%碘伏液擦拭阴道外阴后,阴道外阴喷洒制霉素粉,连用7d.观察3组满意度、疗效及真菌镜检情况.结果 制霉素粉组与联合用药组用药第1、3、6d后满意度比较差异无统计学意义(P>0.05),制霉素粉组、联合用药组治疗后

  2. Analysis of bacterial vaginosis and vulvovaginal candidiasis infection in women and its related factors%细菌性阴道病及假丝酵母菌病的感染情况及相关因素分析

    李素萍; 袁超燕; 陈兰; 武玉莲

    2014-01-01

    Objective To investigate bacterial vaginosis (BV)and vulvovaginal candidiasis (VVC)in women and to analyze its related factors.Methods The conditions of Physical examina-tion of 1967 female patients in the region from 2011 to 2013 were analyzed,and questionnaire was used to explore the relevant factors that affect the occurrence of BV and VVC.Results There were 282 patients with vulvovaginal yeast disease with the detection rate of 14.34%(282 /1967);231 patients were detected with bacterial vaginosis with 11.74% of detection rate (231 /1967);The higher detection rate of vulvovaginal candidiasis occurred among 25 to 35 year old and there was no significant difference in the occurrence of BV (P >0.05);Logistic analysis revealed that abortion was the risk factors that affect the treatment of VVC and BV;The man cooperated with the treatment and used condoms as protective factor as the safe factor.Conclusion VVC and BV are associated with vaginal microflora imbalance,so the patients should pay attention to healthy hy-giene habits as well as focus on treatment of the partners to block cross-infection and ensure good treatment effect.%目的:了解本地区女性细菌性阴道病(BV)及外阴阴道假丝酵母菌病(VVC)的发病情况及影响疾病的相关因素。方法回顾性分析2011-2013年本院1967例女性体检情况,并进行统计分析,采用问卷调查方式分析影响 BV、VVC 发病的相关因素。结果1967例体检检出282例女性 VVC,检出率14.34%(282/1967);检出231例 BV,检出率11.74%(231/1967);VVC 以25~35岁性活跃期年龄段检出率较高,BV 发病率各年龄层差异无统计学意义(P >0.05);VVC 及 BV 治疗效果及疗效影响因素经 Logistic 分析,流产为影响治疗效果的危险因素;男方配合治疗及使用避孕套为康复效果的保护性因素。结论 VVC 与 BV 均与阴道微生态失衡相关,治疗中应注意指导患者养成健康的卫

  3. Analysis and research on treatment and course for recurrent vulvovaginal candidiasis%复发性外阴阴道假丝酵母菌病治疗方案及疗程的研究分析

    刘淑辉

    2013-01-01

    目的 探讨研究复发性外阴阴道假丝酵母菌病治疗方案及疗程.方法 将我院从2011年9月至2012年6月收治的300例复发性外阴阴道假丝酵母菌病患者按照随机数表法均分为3组,A组100例采用硝酸咪康唑栓200 mg/d治疗7~10d,B组100例采用硝酸咪康唑栓400 mg/d治疗4~7d,C组100例在B组基础上在第4日、第7日和第10日口服氟康唑150 mg.分别统计记录治疗后2d、7d、14 d的真菌转阴率和用药依从性,并根据临床症状评判治疗疗效;随访跟踪6个月,统计复发率.结果 三组患者在2d、7d、14 d的真菌转阴率和复发率上组间比较差异无统计学意义(P>0.05);在用药依从性和症状消失时间上,B、C两组优于A组,C组治疗有效率为97%,高于A组、B组,组间比较差异有统计学意义(P<0.05).结论 口服用药联合局部用药能有效提高复发性外阴阴道假丝酵母菌病治疗疗效,短期安全高浓度用药能提高用药依从性.%Objective To investigate the treatment and course for recurrent vulvovaginal candidiasis.Methods 300 patients were divided into 3 groups according to the random number table:group A of 100 patients with miconazole nitrate suppository 200mg/d treatment for 7-10 d,100 cases of group B miconazole nitrate suppository 400 mg/d treatment for 4-7 days,the group C,100 cases on the basis of group B in the 4th,the 7th and the 10th day of oral fluconazole 150 mg.We collected the data of treatment efficience.Results The 2 d,7 d,14 d of fungal negative rate and recurrence rate in the patients of three groups had no statistical significant difference (P>0.05); for the medication compliance and symptoms,group B and C were better than those in group A,group C treatment response rate was 97%,higher than that of group A and group B,the difference between the groups was statistically significant (P<0.05).Conclusion The combined topical oral medication can effectively improve the recurrent vulvovaginal

  4. Avaliação clínica e laboratorial do gel da Uncaria tomentosa (Unha de Gato sobre candidose oral Clinical and laboratorial evaluation of Uncaria tomentosa (Cat's Claw gel on oral candidiasis

    Leonardo Costa de Almeida Paiva

    2009-06-01

    Full Text Available Na odontologia, a fitoterapia já vem sendo utilizada com sucesso há vários anos. Trata-se de um meio terapêutico que apresenta como vantagem sobre as medicações alopáticas o fato de apresentar reações adversas mínimas. A Uncaria tomentosa é uma planta indígena da floresta Amazônica e de outras áreas tropicais da América do Sul e Central. Tem aplicação no tratamento de diversas patologias, entre elas a candidose. Este trabalho avalia clínico e laboratorialmente a ação do gel da Uncaria tomentosa em pacientes portadores de candidose na cavidade oral. Foram selecionados 20 pacientes que apresentaram clínico e laboratorialmente infecção pelo Candida. Os mesmos foram divididos em 2 grupos. O grupo-teste (Uncaria tomentosa/Imuno-Max Gel, composto por 10 pacientes, foi orientado a utilizar o gel da Uncaria tomentosa, sobre as lesões na cavidade oral, 3x ao dia por um período de 14 dias. O grupo-controle (Miconazol/Daktarin Gel utilizou a medicação da mesma forma prescrita para o grupo-teste. Após o período de tratamento, os pacientes retornaram para nova avaliação clínica e laboratorial. A Uncaria tomentosa mostrou ser um fitofármaco promissor na odontologia, apresentando vantagem sobre o miconazol de não ter provocado reações adversas nos pacientes, uma vez que, 40% dos pacientes do grupo-controle, apresentaram reações indesejáveis.In dentistry, the phytotherapy is already being used successfully for some years now. It is about a promising therapeutical way in the pharmaceutical field, having as advantage on pharmacotherapy medications the fact to present minimum adverse reactions. The Uncaria tomentosa is an aboriginal plant of the Amazonian forest and other tropical areas of the South and Central America. It has application in the treatment of several pathologies, including candidiasis. This work evaluates, clinical and laboratorial, the action of the Uncaria tomentosa gel in the oral cavity candidiasis

  5. Chronic mucocutaneous candidiasis: characterization of a family with STAT-1 gain-of-function and development of an ex-vivo assay for Th17 deficiency of diagnostic utility.

    Dhalla, F; Fox, H; Davenport, E E; Sadler, R; Anzilotti, C; van Schouwenburg, P A; Ferry, B; Chapel, H; Knight, J C; Patel, S Y

    2016-05-01

    Chronic mucocutaneous candidiasis (CMC) is characterized by recurrent and persistent superficial infections, with Candida albicans affecting the mucous membranes, skin and nails. It can be acquired or caused by primary immune deficiencies, particularly those that impair interleukin (IL)-17 and IL-22 immunity. We describe a single kindred with CMC and the identification of a STAT1 GOF mutation by whole exome sequencing (WES). We show how detailed clinical and immunological phenotyping of this family in the context of WES has enabled revision of disease status and clinical management. Together with analysis of other CMC cases within our cohort of patients, we used knowledge arising from the characterization of this family to develop a rapid ex-vivo screening assay for the detection of T helper type 17 (Th17) deficiency better suited to the routine diagnostic setting than established in-vitro techniques, such as intracellular cytokine staining and enzyme-linked immunosorbent assay (ELISA) using cell culture supernatants. We demonstrate that cell surface staining of unstimulated whole blood for CCR6(+) CXCR3(-) CCR4(+) CD161(+) T helper cells generates results that correlate with intracellular cytokine staining for IL-17A, and is able to discriminate between patients with molecularly defined CMC and healthy controls with 100% sensitivity and specificity within the cohort tested. Furthermore, removal of CCR4 and CD161 from the antibody staining panel did not affect assay performance, suggesting that the enumeration of CCR6(+) CXCR3(-) CD4(+) T cells is sufficient for screening for Th17 deficiency in patients with CMC and could be used to guide further investigation aimed at identifying the underlying molecular cause. PMID:26621323

  6. An Evaluation of Butoconazole Nitrate 2% Site Release® Vaginal Cream (Gynazole-1® Compared to Fluconazole 150 mg Tablets (Diflucan® in the Time to Relief of Symptoms in Patients With Vulvovaginal Candidiasis

    Campbell K. Skokos

    2005-01-01

    Full Text Available Background. It is estimated that as many as 13 million cases of vulvovaginal infection occur in the United States annually, the majority of which are the result of Candida albicans infection. The symptoms of vulvovaginal infections are often painful and distressing to the patient. The objective of this study was to compare the time to symptomatic relief of vulvovaginal candidiasis (VVC with butoconazole nitrate 2% Site Release® vaginal cream (Gynazole-1® and oral fluconazole 150 mg tablets (Diflucan®.

  7. 乳杆菌活菌胶囊联合克霉唑治疗妊娠期外阴阴道假丝酵母菌病的疗效观察%Observation about curative effect of lactobacillus and clotrimazole on vulvovaginal candidiasis in pregnancy

    邱海凡

    2011-01-01

    目的:观察乳杆菌活茵胶囊(定君生胶囊)配合克霉唑阴道片用于妊娠期外阴阴道假丝酵母茵病的疗效.方法:从2010年1月至12月间产科门诊的孕妇中抽取112例外阴阴道假丝酵母茵病患者,随机分为2组.观察组采用乳杆茵活茵胶囊和克霉唑阴道片联合治疗,对照组单用克霉唑阴道片治疗,比较2组疗效.治疗后10,30,60 d对其疗效及阴道分泌物镜检结果进行比较,观察其疗效及复发性.结果:2组10d治愈率差异无统计学意义(P>0.05),30 d及60 d的复发率差异有统计学意义(P<0.05).结论:乳杆茵活茵胶囊和克霉唑阴道片联合治疗妊娠期外阴阴道假丝酵母茵病效果好,复发率低.%OBJECTIVE To investigate the curative effect of lactobacillus combined with clotrimazole on vulvovaginal candi-diasis in pregnancy. METHODS 112 pregnant patients with vulvovaginal candidiasis were randomly divided into two groups from the outpatient department during January 2010 to December 2010. The 56 patients in treatment group were treated with lactobacillus and clotrimazole, and the 56 patients in the control group were treated with clotrimazole alone. Clinical effect, mincroscopy examination of vaginal discharge and recurrence of vulvovaginal candidiasis in pregnacy were compared at the 10th, 30th and 60th after treatment. RESULTS There was no significant difference in the cure rate between the two groups 10th after the treatment (F>0. 05) , while there was a significantly difference in relapse rate between the two groups 30th and 60th after treatment CONCLUSION The lactobacillus and clotrimazole have long- lasting curative effect and low relapse rate in the treatment of vulvovaginal candidiasis in pregnancy.

  8. 系统性红斑狼疮患者合并甲真菌病、口腔念珠菌病的临床资料分析%Clinical Analysis of Onychomycosis and Oral Candidiasis in Systemic Lupus Erythematosus Patients

    黄远忠; 董正蓉; 林伯盛; 马丹晓

    2011-01-01

    目的 探讨分析系统性红斑狼疮(SLE)患者合并甲真菌痛、口腔念珠菌病的情况及特点.方法 观察176例SLE患者的甲真菌病及口腔念珠菌病的危险因素及其致病真菌构成情况.结果 176例SLE患者中,104例合并真菌感染.甲真菌病61例(34.66%),口腔念珠菌病69例(39.20%),其中二者同时发生26例(14.77%).甲真菌病的主要病原体为红色毛癣菌,临床类型主要为远端侧位甲下型(52.46%).口腔念殊菌病主要病原体为白念珠菌.甲真菌病的患病率随着SLE病程延长而增加.口腔念珠菌病的患病率受泼尼松每日用量、接受甲强龙冲击治疗、使用广谱抗生素的影响.使用免疫抑制剂及病情严重程度对二者的发生均有影响.结论 SLE易发生甲真菌病和口腔念珠菌病,要早期检查并关注存在危险因素患者的甲及口腔黏膜变化.%Objective To investigate the prevalence of onychomycosis and oral candidiasis in systemic lupus erythematosus (SLE) patients. Methods Incidence of onychomycosis and oral candidasis were observed in 176 SLE patients. Constituent ratio of the pathogenic fungi and the risk factors of the two mycoses were also included. Results One hundred and four SLE patients had concomitant mycoses. Sixty-one were onychomycosis ( 34.66% ), 69 oral candidiasis ( 39.20% ), 26 with both mycoses. Trichophyton rubrum and Candida albicans were the major pathogens of onychomycosis and oral candidiasis, respectively. Onychomycosis was correlated with the duration of SLE, immunosuppressive drug use, and severity of SLE. While oral candidiasis was correlated with prednisone dose, methylprednisolone pulse therapy, and broad-spectrum antibiotics. Nail and oral changes should be noted early in those SLE patients with risk factors, immunosuppressive drug use, and severity of SLE. Conclusion SLE patient is susceptible to onychomycosis and oral candidiasis. Nail and oral changes should be noted early in those SLE

  9. Ciclopirox olamine in treatment of vulvovaginal candidiasis: a randomized controlled study%环吡酮胺治疗外阴阴道假丝酵母菌病的随机对照研究

    王林; 段利利; 冯媛; 王欢; 武晓凤; 王小亚

    2015-01-01

    目的 研究环吡酮胺阴道乳膏治疗外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)的疗效.方法 根据纳入标准和排除标准,于2012年1月至2013年10月共入选VCC患者605例,随机分配进入试验组和对照组.试验组阴道给予环吡酮胺乳膏5 g/d(有效成分环吡酮胺0.05 g/d),连用7d;对照组阴道给予硝酸咪康唑栓,200mg/d,连用7d.比较两组临床应用效果.结果 最终共有586例患者完成试验,两组的均衡性良好.试验组和对照组用药24 h内的症状缓解率分别为78.25%(223/285)和41.86%(126/301),差异有统计学意义(P<0.01);停药1周后总有效率分别为91.93%(262/285)和85.05%(256/301),差异有统计学意义(P<0.01).试验组204例治愈患者在停药后3、6个月的复发率分别为0.49%(1/204)和3.92%(8/204),而对照组205例治愈患者的相应复发率分别为7.32%(15/205)和14.15%(29/205),两组比较差异有统计学意义(P<0.01).两组不良反应均轻微.结论 环吡酮胺阴道乳膏治疗VVC较硝酸咪康唑更为有效,且可明显降低复发率.

  10. 肤外洗药联合克霉唑预防念珠菌性外阴阴道病复发的疗效观察%Fuwai-Xiyao combinate with clotrimazole efficacy for prevention of recurrent vulvovaginal candidiasis

    谭敏秀; 刘东文; 李怀国; 陈淑映; 庞卓超

    2014-01-01

    目的:评价肤外洗药联合克霉唑预防念珠菌性外阴阴道炎(vulvovagi-candidiasis, VVC)复发的疗效和安全性。方法:选取本院就诊的患念珠菌性外阴阴道炎的患者73例,随机分组,每组在急性期都用克霉唑治疗,然后以近期临床痊愈者用肤外洗药作为巩固治疗方案的作为治疗组,以不用肤外洗药巩固治疗的作为对照组,比较两组的复发率及不良反应。结果:两组治疗后随访6个月,治疗组复发率明显低于对照组(P<0.05)。结论:肤外洗药预防VVC患者的复发效果好,值得临床推广应用。%Objietive:To evaluate and safety of the Fuwai-Xiyao combinate with clotrimazole to prevent recurrent of Candida vulvovaginitis. Methods:The hospital has suffering from vulvovaginal candidiasis patients of 73 cases,and randomized, two are in the acute phase treatment with clotrimazole,acute onset after cure the Fuwai-Xiyao the consolidation treatment as treatment group,with no the Fuwai-Xiyao to consolidate the treatment as control group, Comparison of the two groups of recurrence and adverse reactions . Results: The recurrence rate for 6 months of treatment group was significantly lower than the control group (P<0.05). Conclusions:Fuwai-Xiyao for prevention of VVC recurrence is worthy of clinical application for good effect.

  11. The microecological evaluation of vaginal microflora in the women of serious vulvovaginal candidiasis%重度外阴阴道假丝酵母菌病阴道微生态分析

    吴文湘; 冯佳; 廖秦平

    2012-01-01

    Objective To investigate the microecological evaluation of vaginal microflora in the women of SVVC. Methods 150 women of SVVC were studied by collecting vaginal secretion, and the vagina pH and microecological evaluation based on Gram stained smear were tested. Results ( 1) 74 % of advantage microflora were lactobacillus, and 80. 67 % of the degree of microflora intensive of cases were Ⅱ - Ⅲ76% of the degree of microflora diversity were Ⅱ - Ⅲ. (2) There were 60 cases of cleanness I (40% ) , 53 cases of cleanness II (35. 33% ) , 37 cases of cleanness Ⅲ (24. 67% ). (3) About 68% of them pH was <4. 5. Conclusion SVVC exist a certain degree disorder of vaginal microecosystem, the advantage microflora still are lactobacillus, the degree of microflora intensive and the degree of microflora diversity of the mainly patients are in normal range.%目的 了解重度外阴阴道假丝酵母菌病(serious vulvovaginal candidiasis,SVVC)患者的阴道微生态情况.方法 对2008年8月至2009年3月北京大学第一医院SVVC患者共150例,进行阴道分泌物微生态检测.结果 (1)优势菌仍以乳酸杆菌为主,占74%,阴道菌群密集度多在Ⅱ~Ⅲ级,占80.67%;阴道菌群多样性多在Ⅱ~Ⅲ级,占76%.(2)清洁度为Ⅰ度的患者60例,占40%,Ⅱ度的患者53例,占35.33%,Ⅲ度的患者37例,占24.67%.(3) pH<4.5占68%.结论 重度外阴阴道假丝酵母菌病存在着一定程度的阴道微生态紊乱,但是优势菌仍以乳酸杆菌为主,阴道菌群密集度和多样性大部分仍在正常范围.

  12. 抗真菌药物治疗外阴阴道念珠菌病敏感性的临床研究%Clinical Research of Susceptibility to Antifungal Agents in the Treatment of Vulvovaginal Candidiasis

    郭爱芹

    2012-01-01

    目的 探讨外阴阴道念珠菌病(VVC)的临床特征及抗真菌药物治疗VVC的敏感性.方法 对我院自2008年1月至2011年8月收治的1000例妇科门诊患者的VVC发病情况进行回顾性分析,观察念珠菌对抗真菌药物的敏感性.结果 通过分离1000株念珠菌,白念珠菌为610例(61%),非白念珠菌为390例(39%).白念珠菌及非白念珠菌对氟康唑和伊曲康唑的敏感度比较差异有统计学意义(P<0.05);对酮康唑和两性霉素B的敏感度比较,差异无统计学意义(P>0.05).结论 白念珠菌是VVC的主要致病菌,非白念珠菌的药物敏感性较白念珠菌低,白念珠菌对抗真菌药物的耐药性不常见.%Objective To discuss the clinical features of vulvovaginal candidiasis( VVC )and the susceptibility to antifungal agents in the treatment. Methods Retrospective analysis of VVC incidence of 1000 gynecological outpatients of our hospital from January 2008 to August 2011 was done, Candida albicans sensitivity to antifungal agents was observed. Results Among the 1000 cases,610 patients( 61% )had Candida albicans,and 390 patients( 39% )had non-Candida albicans. Sensitivity of Candida albicans and non-Candida albicans to fluconazole and itraconazole werestatistically significantly different^ P 0. 05 ). Conclusion The main pathogen of VCC is Candida albicans, non-Candida albicans drug sensitivity is lower than Candida albicans, antifungal drug resistance is not common in Candida albicans.

  13. Study on the relationship between vaginal and intestinal Candida in patients with vulvovaginal candidiasis%外阴阴道假丝酵母菌病患者肠道假丝酵母菌携带与阴道假丝酵母菌感染之间的关系

    林晓丽; 李真; 左绪磊

    2011-01-01

    Objective To investigate the relationship between vaginal and intestinal Candida in patients with vulvovaginal candidiasis by using microbiological and molecular methods. Methods The samples of vaginal discharge and anal swabs were collected from 148 cases with vulvovaginal candidiasis,followed by fungal culture, identification, purification and genome DNA extraction. The genome sequences from respective locations were aligned and typed according to their homology analyzed by internal transcribed spacer (ITS) PCR and random amplified polymorphic DNA (RAPD) PCR. Patients with vulvovaginal infection or those with infections in intestine and vulvovagina were pooled respectively, while the recurrent incidences after local anti-fungal treatments were analyzed. Results Candida albicans is the dominant pathogen in 148 cases with vulvovaginal candidiasis (91.9% , 136/148) ; 33. 1% (49/148) of patients with vulvovaginal candidiasis were infected in both intestine and vulvovagina. While 92% (22/24) of patients with intestinal and vaginal Candida infection showed high homology. The recurrent rate of patients with vulvovaginal candidiasis complicated with concurrent intestinal Candida infection (7/14) was significantly higher than that of solo vaginal infected patients [21% (6/29)] after vaginal treatment (P < 0. 05) . Conclusions The infection of vulvovaginal candidiasis is highly associated with the concurrent infection of intestinal Candida. The recurrent rate is high in patients with vulvovaginal candidiasis with concurrent infection of intestinal Candida after vaginal treatment. The general management to those patients infected by both vulvovaginal and intestinal Candida is necessary in reducing the recurrence of the disease.%目的 采用微生物学和分子生物学方法,研究外阴阴道假丝酵母菌病患者肠道假丝酵母菌携带与阴道假丝酵母菌感染之间的关系.方法 收集2006年4月至2008年2月问上海市长宁区中心医院就诊的148

  14. The clinical effect of treatment with soup finished outside the recurrent vulvovaginal candidiasis in%完带汤治疗复发性外阴阴道假丝酵母菌病 的临床效果观察

    陈晨

    2015-01-01

    目的:分析完带汤治疗复发性外阴阴道假丝酵母菌病的效果观察.方法:研究来自我院在2014年1月—2014年12 月期间的 80 例复发性外阴阴道假丝酵母菌病的患者,分为对照组和观察组,对照组采用克霉唑栓治疗,观察组在对照组基础上运用完带汤口服治疗,而后分析两组治疗效果差异.结果:在治疗有效率上,观察组为95%,对照组为77.5%,同时在复发率上,观察组显著低于对照组,半年的复发率,观察组为20%,对照组为42.5%.结论:通过完带汤来进行复发性外阴阴道假丝酵母菌病的治疗,可以有效提升治疗效果,减少复发率.%Objective: To analyze recurrent vulvovaginal candidiasis finished soup by observing the effects of treatment. Methods: From 80 patients in our hospital with recurrent genital January 2014 to December 2014 during vaginal candidiasis, divided into a control group and the observation group, the control group using clotrimazole suppository treatment, observation treatment group finished adding soup, and then analyze the effect of two treatment differences. Results: In the treatment efficiency, the observation group and 95% in the control group was 77.5%, while the recurrence rate was significantly lower than that observed in the control group, half the recurrence rate was 20% in the observation group, the control group was 42.5% . Conclusion: End with soup for recurrent vulvovaginal candidiasis treatment, can effectively enhance the therapeutic effect and reduce recurrence, combined with the clinical use of conventional medicine does not conflict.

  15. 硝酸咪康唑栓与阴道冲洗联合治疗复发性外阴阴道假丝酵母感染的临床疗效%Clinical Curative Effect Analysis of Nitric Acid Health Thiazole Microphones and Vagina Washing Joint Bolt on the Treatment of Recurrent Vulvovaginal Candidiasis Candida Yeast Infection

    杨建庆; 张跃

    2013-01-01

    Objective: To study the health thiazole bolt and nitric acid microphones vagina washing joint on the treatment of recurrent vulvovaginal candidiasis candida mycoderma bacteria the treatment effect of the disease. Methods: 252 were our cases of recurrent vulvovaginal candidiasis candida mycoderma bacteria patients average divided into two groups, the observation group using nitric acid health thiazole microphones bolt joint vagina washing method treatment group using clotrimazole suppositories treatment, compared to two groups curative effect. Results: The observation group the cure rate of 91.3%; The control group cure rate was 57.9%. Significant difference (P< 0.05). Conclusion:Mi nitric acid health thiazole bolt and vagina washing joint on the treatment of recurrent vulvovaginal candidiasis candida mycoderma bacteria disease treatment effect is remarkable.%  目的:探讨硝酸咪康唑栓与阴道冲洗联合治疗复发性外阴阴道假丝酵母菌病的治疗效果。方法:将我院2009年3月~2012年6月期间收治的252例复发性外阴阴道假丝酵母菌病患者平均分为两组,对照组患者采用克霉唑栓剂治疗,观察组患者使用硝酸咪康唑栓与阴道冲洗法联合治疗,然后比较两组的临床疗效。结果:观察组的治愈率达91.3%;对照组的治愈率为57.9%。组间差异具有统计学意义(P<0.05)。结论:硝酸咪康唑栓与阴道冲洗联合治疗复发性外阴阴道假丝酵母菌病具有较高的临床疗效。

  16. Clinical Efficacy Observation of the Treatment for Patients with Vulvovagi-nal Candidiasis during Pregnancy Period by Using Honghefujie Lotion%红核妇洁洗液治疗妊娠合并外阴阴道假丝酵母菌病的临床疗效探讨

    蔡婷婷; 沈娟娟

    2015-01-01

    目的:探讨红核妇洁洗液治疗妊娠合并外阴阴道假丝酵母菌病的临床疗效和安全性评价,探讨临床价值。方法整群选择2011年6月—2014年6该院收治的妊娠合并外阴阴道假丝酵母菌病患者150例,随机分为研究组和对照组各75例,研究组应用红核妇洁洗液,对照组应用碘化钾及过氧化氢混合溶液进行治疗。疗程结束后对比两组的疗效。结果红核妇洁洗液组治疗总有效率达94.0%,对照组总有效率为66.0%,两组比较差异有统计学意义。结论妊娠合并外阴阴道假丝酵母菌病的治疗,应用红核妇洁洗液效果显著,治愈率高,使用安全、方便、疗程短,值得临床医师推广应用。%Objective To evaluate the efficacy and safety of Honghefujie lotion for the treatment of vulvovaginal candidiasis during pregnancy. Methods 150 cases of patients from 2011 June to 2014 June with vulvovaginal candidiasis in pregnancy, were divided into study group and control group with 75 cases in each group randomly, the research group used honghefujie lotion, treatment control group application of potassium iodide and hydrogen peroxide mixture solution. Compared the efficacy of the two groups be-fore and after treatment. Results The total effective rate of Honghefujie lotion treatment group reached 94%,and the control group’ s total effective rate was 66%, the difference between two groups was significant. Conclusion The combined treatment of vulvo-vaginal candidiasis in pregnancy,application of honghefujie lotion remarkable effect, high cure rate, safe and convenient use, short course of treatment, and is worthy of popularization and application of clinicians.

  17. Observe the Clinical Efficacy of Fluconazole Combined with Nifuratel Nysfungin Vaginal Soft Capsules to Treated the Recurrent Vulvovaginal Candidiasis%氟康唑联合硝呋太尔制霉菌素阴道软胶囊治疗复发性外阴阴道假性酵母菌病疗效观察

    杨育菲; 张健; 陈淑媛

    2012-01-01

      目的:观察氟康唑联合硝呋太尔制霉菌素阴道软胶囊治疗复发性外阴阴道假性酵母菌病的疗效.方法:120例复发性外阴阴道假性酵母菌病患者随机分为三组各40例.治疗组给予氟康唑联合硝呋太尔制霉菌素阴道软胶囊治疗.硝呋太尔组给予硝呋太尔制霉菌素阴道软胶囊治疗.氟康唑组给予氟康唑口服治疗.三组均用3个疗程.比较三组的临床疗效.结果:治疗组的总有效率明显高于二个对照组的总有效率,复发率明显低于二个对照组差异有统计学意义(P<0.05).结论:氟康唑联合硝呋太尔制霉菌素阴道软胶囊治疗复发性外阴阴道假性酵母菌病疗效确切,副反应小,复发率低,适合基层医院推广和应用.%  Objective:To observe the clinical efficacy and safety of Fluconazole combined with Nifuratel Nysfungin Vaginal Soft Capsules on patients with recurrent vulvovaginal candidiasis.Method:120 cases of recurrent vulvovaginal candidiasis were divided randomly into three groups each one had 40 cases. The treatment group took Fluconazole combined with Nifuratel Nysfungin Vaginal Soft Capsules,The Nifuratel group(Control group 1)took Nifuratel Nysfungin Vaginal Soft Capsules,The Fluconazole group(Control group 2)took Fluconazole .The effects after three courses were observed.Result:The total effective rate of treatment group was significantly higher than the two control groups, the recurrence rate of treatment group was significantly lower than the two control groups(P<0.05).Conclusions:Fluconazole combined with Nifuratel Nysfungin Vaginal Soft Capsules of treatment of recurrent vulvovaginal candidiasis is effective,small side effects,low recurrence rate,and suitable for promotion and application of the primary hospital.

  18. Chronic candidiasis - pathogenesis, symptoms, diagnosis and treatment

    Klajn-Laslo Marija

    2009-01-01

    Full Text Available The yeast named Candida normally colonizes the gut and vagina without causing any sign of its presence. It is a commensal and opportune fungus but in certain conditions it turns to be pathogenic, causing chronic disturbances in any part of the body. The pathogenesis is complex, signs and symptoms are non-specific. The colonisation is difficult to distinguish from invasive disease. The current diagnostic methods do not always allow a definitive diagnosis to be made. Treatment is complex, individual and no protocol can be created. The author tries to give an overview of the Candida related problem.

  19. Epidemiology and antifungal resistance in invasive candidiasis

    Rodloff AC

    2011-04-01

    Full Text Available Abstract The epidemiology of Candida infections has changed over the last two decades: The number of patients suffering from such infections has increased dramatically and the Candida species involved have become more numerous as Candida albicans is replaced as an infecting agent by various non-C. albicans species (NAC. At the same time, additional antifungal agents have become available. The different Candida species may vary in their susceptibility for these various antifungals. This draws more attention to in vitro susceptibility testing. Unfortunately, several different test methods exist that may deliver different results. Moreover, clinical breakpoints (CBP that classify test results into susceptible, intermediate and resistant are controver- sial between CLSI and EUCAST. Therefore, clinicians should be aware that interpretations may vary with the test system being followed by the microbiological laboratory. Thus, knowledge of actual MIC values and pharmacokinetic properties of individual antifungal agents is important in delivering appropriate therapy to patients

  20. Vulvovaginal candidiasis in a Flemish patient population

    De Vos, MM; Cuenca-Estrella, M; Boekhout, T; Theelen, B; Matthijs, N; Bauters, T; Nailis, H; Dhont, MA; Rodriguez-Tudela, JL; Nelis, HJ

    2005-01-01

    Increased resistance to fluconazole has been reported in oral, oesophageal and urinary Candida isolates, but this has not been observed commonly in genital tract isolates. The rate of isolation of Candida spp. and their susceptibility to amphotericin B, flucytosine and azoles were determined in a nu

  1. Candidíase oral e leucoplasia pilosa como marcadores de progressão da infecção pelo HIV em pacientes brasileiros Oral candidiasis and hairy leukoplakia as progression markers of HIV infection in Brazilian patients

    Ivan Dieb Miziara

    2004-06-01

    Full Text Available Candidíase oral (CO e leucoplasia pilosa (LP são importantes indicadores da progressão da infecção pelo vírus da imunodeficiência humana (HIV para o quadro de AIDS, principalmente em locais onde exames específicos são inacessíveis. OBJETO: Relacionar CO e LP ao número de células CD4+ e à carga viral (CV em pacientes brasileiros HIV-positivos, confirmando-as como marcadores clínicos confiáveis de progressão da doença. FORMA DE ESTUDO: Coorte longitudinal. CASUÍSTICA E MÉTODO: Avaliamos prospectivamente 124 pacientes HIV-positivos, isentos de terapia antiretroviral. Todos foram submetidos a exame ORL, dosagem de células CD4+ e CV, sendo divididos em dois grupos: P e A, de acordo com a presença ou ausência de CO e LP. Depois de seis meses, os pacientes do grupo A foram subdivididos nos subgrupos P6 (presença de lesões e A6. Dosamos novamente CD4+ e carga viral. Os resultados foram analisados estatisticamente. RESULTADOS: No grupo P (43 pacientes, 28 CO e 15 LP a contagem de células CD4+ foi menor e a carga viral maior em relação ao grupo A (pOral candidiasis (OC and hairy leukoplakia (HL are important markers of HIV (Human Imunodeficiency syndrome infection progression for AIDS, mainly in locals where specific tests are inacessible. AIM: to intertwine OC and HL to CD4+ counting and to viral charge (VC on HIV positive brazilian patients, confirming them as trustworthy clinical markers of the disease progression. STUDY DESIGN: Longitudinal cohort. MATERIAL AND METHOD: we have prospectively evaluated 124 HIV+ patients not in use of antiretroviral therapy. All of them have undertaken otorrhinolaringologic examination and CD4+ and VC counting, being divided in two groups: P and A, accordingly to presence or absence of OC and HL. After six months, patients belonging to the A group were re-divided on groups P6 (presence of lesions and A6 (absence of lesions. Again, CD4+ and VC were counted. The results were statistically

  2. 中西医结合治疗妊娠期外阴阴道假丝酵母菌病临床观察%Clinical Observation of Vulvovaginal Candidiasis in Pregnancy Treated with Integrated Chinese and Western Medicine

    高丽霞; 吉广庆

    2012-01-01

    Objective To observe the clinical efficacy of vulvovaginal candidiasis( WC ) in pregnancy treated with integrated Chinese and western medicine. Methods 96 cases were randomized into two groups. 48 cases in a treatment group were treated with Chinese topical medicine and clotrinazole vaginal suppositories. 48 cases in a control group were treated with 2% topical sodium bicarbonate and clotrinazole vaginal suppositories. 7 - day treatment made 1 session. The continuous 2 sessions of treatment were required. The efficacy and the recurrence rate of the curative cases in 1 month were observed and compared between two groups. Results The therapeutic effect and recurrence rate were different significantly in statistics between two groups. In the treatment group, the total effective rate was 97. 9% and the recurrence rate was only 2. 7%. In the control group, the total effective rate was 89. 6% and the recurrence rate was 15. 6%. The improvements in the clinical manifestations after treatment were different significantly in statistics as compared with those before treatment in either group. The efficacy in the treatment group was superior significantly to that in the control group. Concerning the fungus detection rate in two groups, the long - term fungus detection rate was superior to the short - term one. The result in the treatment was better than that in the control group, presenting the statistical significant difference. Conclusion The integrated Chinese and western medicine relieves apparently the symptoms of WC in pregnancy and has less recurrence. Hence,this therapy deserves to be promoted in clinic.%目的 观察中西药结合治疗妊娠期外阴阴道假丝酵母菌病的临床疗效.方法 96例患者随机分成两组.治疗组48例,中药局部用药加克霉唑栓阴道上药.对照组48例,2%碳酸氢钠局部用药加克霉唑栓阴道上药,7 d为1疗程,连用2个疗程.观察比较两组疗效和治愈者1个月后的复发率.结果 治疗效果和

  3. 支原体检测与治疗对复发性外阴阴道假丝酵母菌病的作用%Role of Mycoplasma Detection and Treatment in Recurrent Vulvovaginal Candidiasis

    熊小琴; 朱虹; 布占红

    2012-01-01

    目的 通过对复发性外阴阴道假丝酵母菌病(RVVC)患者同时进行支原体检测与相应治疗,探讨支原体感染对诱发RVVC中的作用.方法 回顾性分析过去3年收治的250例诊断为RVVC患者,按随机数字表法分为实验组(100例)与对照组(150例),实验组入组同时行支原体检测及药敏试验,实验组RVVC合并支原体阳性者(实验1组)同时予常规抗真菌及抗支原体治疗,实验组RVVC合并支原体阴性者(实验2组)仅给予抗真菌治疗;对照组不检测支原体,治疗仅给予常规抗真菌治疗.结果 实验组中,69%患者(69例)同时合并支原体感染,2者同时治疗后治愈率为92.75%(64例);实验组中支原体阴性者,治愈率为90.32%(28例);对照组治愈率为40.67%(61例).实验1、2组与对照组治愈率比较差异有统计学意义(P<0.01).结论 支原体感染对诱发女性生殖道RVVC有一定作用,给予同时治疗可以降低RVVC复发率.%Objective To investigate the role of mycoplasma infection in recurrent vulvovaginal candidiasis (RVVC) through the detection and treatment of mycoplasma. Methods Data of 250 patients diagnosed with RVVC in past 3 years were retrospectively analyzed. Theses patients were randomly divided into two groups. Patients in experimental group (n=100) underwent mycoplas-ma detection and drug susceptibility testing, and then mycoplasma positive RVVC patients were given conventional anti-fungal and anti-mycoplasma treatment and mycoplasma negative RVVC patients were only given anti-fungal treatment. The control group (n=150) only received conven-tional anti-fungal therapy. Results Among the 100 patients in experimental group, RVVC was complicated by mycoplasma infection in 69 (69%), and clinical cure was achieved in 64 (92.75%). Among mycoplasma negative RVVC patients, clinical cure was achieved in 28 (90. 32%). Among the 150 patients in control group, clinical cure was achieved in 61 (40. 67%). Experiment 1 and 2 compared with

  4. 胸腺五肽对复发性外阴阴道念珠菌病患者IL-12p70和IL-23p19水平的影响及疗效分析%Therapeutic Effect of Thymopentin on IL-12p70 and IL-23p19 Level in Patients with Recurrent Vulvovaginal Candidiasis

    喻楠; 夏毓; 尚元元; 董灵娣

    2011-01-01

    Objective To observe therapeutic effect of thymopentin on IL - 12p70 and IL -23pl9 level and in patients with recurrent vulvovaginal candidiasis. Methods 80 subjects were divided into three groups, namely treatment group, control group and healthy group.IL - 12p70 and IL -23pl9 level in normal peopleand the patients of recurrent vulvovaginal candidiasis were detected by enzyme - linked immunosorbent assay -Double - antibody sandwich method before and after treatment. Results IL - 12p70 and IL -23pl9 levels in pretherapeutic patients with recurrent vulvovaginal candidiasis were significantly lower than those of healthy group, the two groups of patients had the similar level of IL - 12p70 and IL - 23pl9 ( P > 0. 05). After treat-ment, the level of IL - 12p70 and IL -23pl9 in group with thymopentin increased significantly compared with control group(P <0. 01). The therapeutic effect of treatment group(total effective rate; 80. 00% ) was signifi-cantly higher than that of control group (total effective rate: 43. 33 % ) ( P < 0. 05). Conclusion There exists imbalance of Thl/Th2 cell factors in the patients with recurrent vulvovaginal candidiasis. Thymopentin can improve the patients 'immunologic function and enhance the therapeutic effect by rectify the imbalance of the two type of cell factors.%目的 研究观察胸腺五肽对复发性外阴阴道念珠菌病患者阴道分泌物IL-12p70和IL-23p19水平的影响及疗效.方法 采用酶联免疫吸附实验(ELISA)双抗体夹心法检测复发性外阴阴道念珠菌病患者治疗组、对照组治疗前后及正常人阴道分泌物IL-12p70和IL-23p19水平.结果 两组治疗前复发性外阴阴道念珠菌病患者IL-12p70和IL-23p19水平均低于正常人(P<0.01),两组患者间IL-12p70和IL-23p19水平相近(P>0.05).治疗后胸腺五肽组IL-12p70和IL-23p19水平升高,对照组治疗前后水平差异无统计学意义(P>0.05).治疗组的疗效(总有效率80.00%)明显高于对照组的43.33

  5. 克霉唑与制霉菌素栓治疗妊娠期外阴阴道假丝酵母菌病疗效及安全性比较%Efficacy and safety of clotrimazole and nystatin suppository in treatment of vulvovaginal candidiasis during pregnancy

    吴熊军

    2013-01-01

    OBJECTIVE To observe and compare the efficacy and safety of clotrimazole and nystatin suppository in treatment of vulvovaginal candidiasis during pregnancy so as to provide basis for the treatment of vulvovaginal candidiasis during pregnancy. METHODS A total of 124 patients with pregnancy vulvovaginal candidiasis, who enrolled the hospital from Jun 2008 to Mar, were selected and randomly divided into the observation group and the control group with 62 cases in each; the observation group was treated with clotrimazole, the control group with nystatin suppository. The efficacy and safety of medication of the two groups were observed. RESULTS The VVC score of the observation group was obviously higher than that of the control group (P<0. 05). The cure rate of the observation group was 59. 68%, significantly higher than 50% of the control group (P<0. 05). The inefficiency of the observation group was 1.61%, significantly lower than 6. 45% of the control group (P<0. 05) ; the total effective rate of the observation group was 87. 10%, significantly higher than 79. 03% of the control group (P<0. 05). CONCLUSION The efficacy of clotrimazole is superior to that of nystatin suppository in treatment of pregnancy vulvovaginal candidiasis, and the safety of both two drugs is good.%目的 观察比较克霉唑与制霉菌素栓治疗妊娠期外阴阴道假丝酵母菌病的疗效以及安全性,为治疗妊娠期外阴阴道假丝酵母菌病提供临床参考.方法 选取2008年6月-2012年3月患外阴阴道假丝酵母菌病的妊娠期妇女124例,随机分为观察组与对照组,每组62例;观察组给予克霉唑治疗,对照组给予制霉菌素栓治疗,观察比较两组患者治疗后的疗效和用药安全性.结果 观察组患者治疗后VVC评分明显低于对照组(P<0.05);观察组的治愈率为59.68%,明显高于对照组的50.00%(P<0.05);观察组的无效率为1.61%,明显低于对照组的6.45% (P<0.05);

  6. 乳酸杆菌活菌制剂联合克霉唑治疗外阴阴道假丝酵母菌病的Meta分析%Meta-analysis on live lactobacillus preparation plus clotrimazole for treatment of vulvovaginal candidiasis

    冯燕翀; 牛战琴

    2014-01-01

    Objective To evaluate the efficacy of live lactobacillus preparation plus clotrimazole on treatment of vulvovaginal candidiasis by Meta-analysis. Methods Randomized controlled trials on treatment of vulvovaginal candidiasis with live lactobacillus preparation plus clotrimazole published in China from 1966 to 2013 were retrieved for further analysis by Revman4.2 software. Results There was significant difference in the efficacy of vulvovaginal candidiasis treatment between live lactobacillus preparation plus clotrimazole and clotrimazole alone, with the point estimate of combined RR value of 1.06 and confidence interval of 1.03-1.09 (P=0.0001<0.05). There was statistical significance between combined drug use and clotrimazole alone with regard to the recurrence rate within 1, 2, 3 months after follow-up [RR= 036, 95%CI (0.20, 0.66), Z=3.33, P=0.0009; RR=0.29, 95%CI (0.13, 0.61), Z=3.26, P=0.001;RR=0.26, 95%CI (0.09, 0.75), Z=2.48, P=0.01]. Conclusion As for treatment of vulvovaginal candidiasis, live lactobacillus preparation plus clotrimazole has better therapeutic effects and lower recurrence rate than clotrimazole alone.%目的:运用Meta分析方法评价乳酸杆菌活菌制剂联合克霉唑治疗外阴阴道假丝酵母菌病的疗效。方法检索1966~2013年国内发表的乳酸杆菌活菌制剂联合克霉唑治疗外阴阴道假丝酵母菌病的随机对照试验,并使用Revman4.2软件进行分析。结果与克霉唑单一用药相比,联合乳酸杆菌活菌制剂治疗外阴阴道假丝酵母菌病疗效有统计学差异,RR合并值的点估计为1.06,可信区间为1.03~1.09(P=0.0001,即P<0.05);联合用药相比单一克霉唑,随访1、2、3个月后复发率均有统计学差异[RR=036,95%CI(0.20,0.66),Z=3.33,P=0.0009;RR=0.29,95%CI(0.13,0.61),Z=3.26,P=0.001;RR=0.26,95%CI(0.09,0.75), Z=2.48,P=0.01]。结论乳酸杆菌活菌制剂联合克霉唑治疗外阴阴道假丝酵母菌

  7. 两种不同方案治疗复发性外阴阴道假丝酵母菌病的疗效观察%Observation on Curative Effects of Lactobacillus and/or Nystatin in Treatment of Recurrent Vulvovaginal Candidiasis

    席德娣

    2011-01-01

    目的 探讨定君生和(或)制霉菌素栓治疗复发性外阴阴道假丝酵母菌病(RVVC)的临床效果.方法 确诊的130例RVVC患者随机分为两组.观察组65例,在使用定君生和制霉菌素栓一个疗程后,继续使用定君生治疗2疗程;对照组65例,使用制霉菌素栓治疗3疗程.比较两组治疗效果、治疗后的复发率.结果 观察组复发率明显低于对照组(P<0.05),应用定君生未出现严重的不良反应.结论 阴道乳酸杆菌活菌制剂定君生调整阴道菌群失调,可有效地降低RVVC的复发率.%Objective To explore the curative effects of lactobacillus and/or nystatin in treating recurrent vulvovaginal candidiasis (RWC). Methods Totally 130 cases of confirmed recurrent vulvovaginal candidiasis were randomly divided into two groups (each n = 65). The observation group was treated with one course of lactobacillus and nystatin, and then with two courses of lactobacillus. The control group was treated with three courses of nystatin. The curative effects and relapse rates of the two groups were compared. Results The relapse rate in the observation group was significantly lower than that in the control group (P<0.05). No serious adverse reaction was found in the patients treated with lactobacillus. Conclusions Lactobacillus (Ding- Jun- Sheng), which regulates vaginal dysbacteriosis, can effectively reduce the relapse rate of RWC.

  8. 探讨妊娠期外阴阴道假丝酵母菌病症状与妊娠结局关系%Study on the Relationship Between the Period of Vulvovaginal Candidiasis Symptoms of Pregnancy and Pregnancy Outcome

    赵庆霞

    2015-01-01

    Objective To study the analysis of gestational vulvovaginal candidiasis (VVC) symptoms.MethodsIn our hospital from 2012 to 2013 with vulvovaginal candidiasis positive diagnosis of 136 patients with gestational age over 35 weeks, these patients were divided into 60 cases in the observation group, are symptomatic patients, the remaining 76 cases of asymptomatic patients assigned to the control group. On the pregnancy outcome of two groups of patients were observed. ResultsThe observation group patients appear premature rupture of membranes, puerperal infection and cesarean rates far higher than those of the control group, P0.05.ConclusionVVC can cause symptoms of pregnancy with premature rupture of membranes, the cesarean section rate increased adverse pregnancy outcome, early diagnosis and treatment is very necessary.%目的 研究分析妊娠期外阴阴道假丝酵母菌病(VVC)症状.方法 2012至2013年我院有136例孕龄超过35周的外阴阴道假丝酵母菌病阳性诊断患者,将这些患者中60例分到观察组中,均是有症状的患者,其余76例无症状患者分到对照组中.对两组患者的妊娠结局进行观察.结果 观察组患者出现胎膜早破、产褥感染和剖宫产的几率要比对照组高,P0.05.结论 妊娠期有症状VVC可致胎膜早破、剖宫产率等不良妊娠结局增高,早期诊断和治疗十分必要.

  9. Oral or intravaginal antifungal for the treatment of recurrent vulvovaginal candidiasis in China: a systematic review%口服与阴道使用抗真菌药治疗复发性霉菌性阴道炎疗效的Meta分析

    于洋; 徐红

    2013-01-01

    Objective:To evaluate the clinical efficacy of itraconazole,fluconazol and intravaginal medicine for women with recurrent vulvovaginal candidiasis.Methods:Many databases were searched,including CENTRAL (The Cochrane Library 2011),PubMed (1979 to January 2011),CBMdisc (1979 to January 2011),CNKI (1979 to January 2011),QINGHUATONGFANG DATABASE (1979 to January 2011) and WANFANG DATABASE (1979 to January 2011).Relevant journals and references were also manually searched.Randomized controlled trials (RCTs) was screened on itraconazole,fluconazol and intravaginal antifungal for the treatment of women with recurrent vulvovaginal candidiasis.Two reviewers independently accomplished study selection and quality assessment,data extraction and analysis according to the Cochrane Handbook for systematic reviews of interventions.RevMan 5.0 was used to account the data.Results:18 RCTs were included.The Meta-analysis showed that the Itraconazole or fluconazol with intravaginal antifungal was more effective than the other treatment.Compared with the intravaginal antifungal,the combined treatment was the same safety.Conclusion:Combined treatment is good choice for curing the recurrent vulvovaginal candidiasis.%目的:系统评价应用伊曲康唑、氟康唑、阴道用药治疗复发性霉菌性阴炎疗效.方法:遵照Cochrane系统评价方法,检索中英文数据库、专业杂志等.应用RevMan 5.0软件进行Meta分析.结果:18篇临床对照研究Meta分析结果显示:氟康唑与阴道联合用药与阴道用药疗效比较差异有统计学意义[达克宁OR=5.54,95%CI (2.49,12.30),P<0.0001;制霉菌素OR=4.38,95%CI (1.82,l0.53),P=0.00l],氟康唑联合用药与阴道用药不良反应差异无统计学意义[OR=1.93,95% CI (0.58,6.42),P=0.28].伊曲康唑与阴道用药比较不良反应差异有统计学意义[OR=19.10,95%CI (2.51,145.15),P=0.004].结论:伊曲康唑、氟康唑与阴道联合用药能更有效的治疗复发性霉菌性阴道炎,且不

  10. The clinical analysis of traditional Chinese and western medicine combined with health care education in the treatment of vulvovaginal candidiasis%中西医联合健康护理教育治疗外阴阴道假丝酵母菌病的临床分析

    关冰

    2015-01-01

    目的:探讨中西医联合健康护理教育治疗外阴阴道假丝酵母菌病的临床效果。方法选取河南省省直第一医院2012~2014年收治的90例外阴阴道假丝酵母菌病患者的临床资料,根据不同治疗方法分为3组,A组30例患者采取西医治疗,B组30例患者采取西医治疗结合健康护理教育, C组30例患者采取中西医结合治疗并接受健康护理教育。对比分析3组患者的治疗有效率及复发率。结果 C组患者的治疗有效率96.7%(29/30)高于A、B两组[60.0%(18/30)、76.7%(23/30)],复发率3.3%(1/30)低于A、B两组[43.3%(13/30)、20.0%(6/30)](P<0.05);B组患者的治疗有效率高于A组,复发率低于A组(P<0.05)。结论中西医结合治疗联合健康护理教育能够有效改善外阴阴道假丝酵母菌病患者的临床症状,提高治愈率,减少复发率。%Objective To explore the effect of traditional Chinese and western medicine combined with health care education in the treatment of vulvovaginal candidiasis. Methods Clinical data of 90 patients with vulvovaginal candidiasis in The First Hospital of Henan Province were chosen, all cases were divided into 3 groups according to different treatments with 30 cases in each group. Patients in Group A were treated with western medicine, patients in Group B were treated with western medicine combined with health care education, while patients in Group C were treated with traditional Chinese and western medicine treatment combined with health care education. The effective rates of treatment and recurrence rates between 3 groups were compared. Results The effective rate of treatment in Group C [ 96. 7 % ( 29/30 ) ] was higher than Groups A and Group B [ 60. 0 %( 18/30 ) , 76. 7 %( 23/30 ) ] , and recurrence rate [3. 3%(1/30)] was less than Groups A and B [43. 3%(13/30), 20. 0%(6/30)] (P<0. 05). The effective rate of treatment in Group B was higher than Group A while disease recurrence rates was less than

  11. 定君生治疗外阴阴道假丝酵母菌病的临床疗效及复发率比较%The clinical effects of Live Preparation of Lactobacillus in treatment of vulvovaginal candidiasis and the recurrence rate

    史红钗

    2012-01-01

    Objective To explore the clinical effects of Live Preparation of Lactobacillus in the treatment of vulvovaginal candidiasis and the recurrence rate. Method 86 cases with vulvovaginal candidiasis were divided into control group (n = 43) and treatment group (n =43). The patients of control group were treated with Clorimazole Suppositories and those of treatment group with live Preparation of Lactobacillus plus clorimazole suppositories for 10 days. The clinical effects and recurrence rates were analyzed. Result The total effective rate of treatment group was higher than control group (83.72% vs 65. 12% , P <0. 05) on the 5th day. There was no significant difference in total effective rates on the 10th day (95.35% vs 90. 7.0% , P > 0.05 ). After treatment, the clinical symptoms scores of two groups had fallen significantly on the 5 th and 10th day ( P < 0. 05 ). The clinical symptoms score was lower in treatment group than in control group on 5 th day ( P < 0. 05). There was no significant difference in clinical symptoms scores on 10th day (P > 0. 05). The recurrence rate of treatment group was lower than that of the control group (9.30% vs 27.91 % , P < 0. 05 ). Conclusion Both Live Preparation of Lactobacillus and Clolrimazole Suppositories are effective in treatment of vulvovaginal candidiasis, and can reduce the recurrence rate.%目的 探讨定君生治疗外阴阴道假丝酵母菌的临床疗效及复发情况.方法 将86例外阴阴道假丝酵母菌病患者随机分为对照组(n=43)和治疗组(n=43),对照组阴道塞入克霉唑栓,治疗组在对照组的基础上阴道塞入定君生胶囊,连续应用10 d.对两组临床疗效及复发率进行分析.结果 用药第5天,治疗组总有效率显著高于对照组(83.72%vs 65.12%,P<0.05);用药第10天,两组总有效率比较差异无统计学意义(95.35%vs 90.70%,P>0.05);经治疗后,两组患者用药第5天和用药第10天其临床症状评分均显著下降,与治疗

  12. 乳酸杆菌活菌胶囊联合抗真菌药物治疗复发性外阴阴道白假丝酵母菌病的临床观察%Lactobacillus preparation combined antifungal drugs in the treatment of recurrent vulvovaginal candidiasis recurrentclinical observation

    叶珊珊; 邢秀伟; 黄瑞丽

    2016-01-01

    Objective To study the lactic acid bacteria living bacterium capsule in diabetic pa-tients with recurrent vulvovaginal candidiasis vaginal role in the treatment of disease.Methods A total of 95 patients with recurrent vulvovaginal candidiasis of diabetic patients according to the mechanical sampling method were randomly divided into two groups,conventional treatment group (47 cases,the conventional fall blood sugar,the antifungal treatment,dingjunsheng treatment group 48 cases,on the basis of conventional treatment combined with lactic acid bacteria living bacterium capsule,observe two groups before and after treatment in patients with clinical symptoms,signs,vaginal discharge was normal and culture change and relapse,and compared with the conventional treatment group.Results After the initial treatment on jun the curative effect of treatment group and control group no differ-ence (P > 0.05),but the prince was born recurrence rate reduced compared with the conventional treatment group (P <0.05).Conclusion Live lactobacillus capsule in the treatment of diabetic pa-tients with recurrent vulvovaginal candidiasis can reduce the recurrence rate,worthy of clinical use.%①目的探讨乳酸杆菌活菌胶囊(定君生)治疗糖尿病患者复发性外阴阴道白假丝酵母菌病(VVC)的效果。②方法将95例患有复发性 VVC 的糖尿患者按机械抽样法随机分为两组,常规治疗组47例,采用常规降血糖、抗真菌综合治疗;定君生治疗组48例,在常规综合治疗基础上加用乳酸杆菌活菌胶囊。观察比较两组患者治疗前后临床症状、体征、阴道分泌物常规及培养变化情况、复发情况。③结果初始治疗结束后定君生治疗组的治疗效果与对照组比较,差异无统计学意义(P >0.05),但定君生治疗组的复发率较常规治疗组降低,差异有统计学意义(P <0.05)。④结论乳酸杆菌活菌胶囊治疗糖尿病患者复发性 VVC 可以减少其复发率。

  13. The clinical effect of reactive lactobacillus pharmacy combined with clotrimazole in curing vulvovaginal candidiasis occurring during pregnancy%克霉唑联合乳酸杆菌活性制剂治疗妊娠期外阴阴道假丝酵母菌病疗效观察

    张丽娟; 宁玉梅; 朱玲

    2014-01-01

    Objective To understand the clinical effect of reactive lactobacillus pharmacy combined with clotrimazole in curing vulvovaginal candidiasis during pregnancy.Method 127 patients with the disease were randomly divided into an experimental group and a control group.The experimental group were given reactive lactobacillus pharmacy combined with clotrimazole,while the control group were given clotrimazole only.The clinical effects of the medicines on the two groups one week after treatment and recurrence rate within one month and two months were recorded and analyzed.Findings One week after treatment,the total clinical efficacy of the designated medicine on the experimental group was 95.38%.The efficacy of clotrimazole on the control group was 93.55%.The difference was not statistically significance(P>0.05).One month and two months after treatment,the recurrence rates of the experimental group were 2.17%and 6.52%respectively, while the recurrence rates of the control group were 15.38% and 25.64% respectively.The differences between the two groups in recurrence rate were statistically significant (P<0.05).Conclusion The clinical effect of reactive lactobacillus pharmacy combined with clotrimazole in curing vulvovaginal candidiasis during pregnancy was found to bear no prominent difference than that of the clotrimazole.However, it has great advantage in preventing the recurrence of the disease.%目的:探讨克霉唑联合乳酸杆菌活性制剂治疗妊娠期外阴阴道假丝酵母菌病( vulvovaginal candidiasis ,VVC)的临床疗效。方法将127例妊娠期VVC患者随机分成观察组和对照组,观察组采用克霉唑联合乳酸杆菌活性制剂治疗,对照组单用克霉唑治疗,观察治疗结束后1周的临床疗效和1个月、2个月的复发率。结果治疗结束后1周,观察组的总有效率为95.38%,对照组为93.55%,差异无统计学意义( P>0.05)。观察组1个月、2个月复发率分别为2.17

  14. Curative effects of Fluconazole combined with Viable Lactobacillus Preparation in treatment of vulvovaginal candidiasis%氟康唑联合乳酸杆菌活菌制剂用于外阴阴道假丝酵母菌病疗效

    赵琴

    2011-01-01

    Objective: To investigate the clinical significance of Fluconazole combined with Viable Lactobacillus Preparation in the treatment of vulvovaginal candidiasis. Methods: 80 patients with vulvovaginal candidiasis treated in our hospital were randomly divided into the experimental group and the control group. The experimental group was treated with Fluconazole combined with Viable Lactobacillus Preparation and the control group was treated with Fluconazole only. The curative effects of the two groups were observed. Results: In the experimental group, 29 patients were cured, with the cure rate of 72.5%, and in the control group, 23 patients were cured, with the cure rate of 57.5%. The difference between the two groups was statistically significant (P<0.05). In the experimental group, 40 patients had valid curative effects, with the effective rate of 100%, and in the control group, 35 patients had valid curative effects, with the effective rate of 87.5%. The difference between the two groups was statistically significant (P<0.05). Conclusion: Compared with the simple anti-fungal treatment, Fluconazole combined with Viable Lactobacillus Preparation can obtain more preferable curative effects and effectively reduce the pain of patients in the treatment of vulvovaginal candidiasis, thereby worthy of clinical application and promotion.%目的:探讨氟康唑联合乳酸杆菌活菌制剂在外阴阴道假丝酵母菌病治疗中的临床意义.方法:将我院经治的外阴阴道假丝酵母菌病80 例,随机分为实验组和对照组,实验组应用氟康唑联合乳酸杆菌活菌制剂治疗,对照组单纯应用氟康唑治疗,观察两组的治疗效果.结果:在实验组中有29 例治愈,治愈率为72.5%,对照组为23 例,治愈率为57.5%,两者比较差异有统计学意义(P<0.05);在实验组中40 例患者治疗均有效,有效率达100%,对照组中35例治疗有效,有效率达87.5%,两者比较差异有统计学意义(P<0.05).结论:氟康唑联合

  15. Recurrent Vulvovaginal Candidiasis Treated with Modified WanDaiTang Combined with External Treatment: A Report of 68 Cases%完带汤加味合外治法治疗复发性外阴阴道假丝酵母菌病68例

    杜敏

    2012-01-01

    目的 观察口服中药完带汤加味联合外用中药熏洗及阴道上药,治疗外阴阴道假丝酵母菌病的疗效.方法 将136 例外阴阴道假丝酵母菌病患者随机分为2 组.治疗组68 例,口服中药完带汤加味,自制中药方熏洗外阴并配合阴道上药治疗;对照组68 例,除口服完带汤加味外,其余治疗同对照组.观察2 组治疗后疗效,并对停药后1、2、3 个月的患者进行回访分析.结果 治疗组总有效率98.5%;对照组总有效率89.7%.2 组比较有统计学意义(P<0.05).停药后1 个月,2 组均无复发;2 个月后治疗组2 例复发,对照组4 例复发;3 个月后治疗组3 例复发,对照组9 例复发.2 组复发率比较差异有统计学意义(P<0.05).结论 完带汤加味合外治法治疗复发性外阴阴道假丝酵母菌病效果好,复发率低.%Objective: To explore therapeutic effects of modified WanDaiTang combined with herb fumigating and application in treating vulvovaginal candidiasis. Method: 136 cases of vulvovaginal candidiasis were chosen and divided into treatment group which orally took modified WanDaiTang combined with herb fumigating and application and control group administered with herb fumigating and application. Curative effects for both groups were observed after the treatment and return visits of the patient were analyzed in one month, two months and three months after withdrawal of drugs. Result: Total effective rate of treatment group was 98.5%, higher than 89.7% of control group with statistical meaning (P <0.05). The patients in both groups were cured without relapse in one month after withdrawal of drugs; two patients in treatment group and four patients in control group had relapse in two months; three patients in treatment group and nine cases in control group had relapse in three months. The comparison of recurrence rates of two groups suggested statistical meaning (P<0.05). Conclusion: Modified WanDaiTang combined with external treatment is

  16. 保妇康凝胶联合乳酸菌素阴道胶囊治疗妊娠合并外阴阴道假丝酵母菌病的临床观察%Clinical Observation of Baofukang Gel Combined with Lactobacillus Vagina Capsule in Treatment of Pregnancy and Vulvovaginal Candidiasis

    田慧敏; 黄彦霞; 宋志红; 苏淑敏

    2013-01-01

    Objective:To explore the clinical curative effect and the safety of Baofukang gel combined with lactobacillus vagina capsule in treatment of vulvovaginal candidiasis. Method:Two hundred and seventy-two cases of pregnant women with vulvovaginal candidiasis were selected and randomly divided into two groups. Group A were given Baofukang gel combined with lactobacillus vagina capsule,while Group B were given only Baofukang gel. After using the medicines,the curative effect,drug’s side effect and recurrence rate between the two groups were compared. Result:The effective rate was 89.92%in group A while it was 76.22%in Group B after treatment,the total effective rate in Group A was significantly higher than that in Group B(P<0.05);The recurrence rate in Group A was 5%,while it was 19%in Group B,the difference was significant(P<0.01). Conclusion:The curative effect of Baofukang gel combined with lactobacillus vagina capsule in the treatment of vulvovaginal candidiasis is definite;It has little side effect and low recurrence rate.%目的:观察保妇康凝胶联合乳酸菌素阴道胶囊治疗妊娠合并外阴阴道假丝酵母菌病的临床疗效和安全性。方法:选择外阴阴道假丝酵母菌病的孕妇272例,随机分成两组。其中A组为保妇康凝胶联用乳酸菌素阴道胶囊组,用保妇康凝胶联合乳酸菌素阴道胶囊治疗, B组为保妇康凝胶组,单一应用保妇康凝胶治疗。用药后观察并比较两组治疗疗效、药物副作用及复发率情况。结果:治疗结束后,A组有效率89.92%,B组为76.22%,A组的总有效率明显高于B组,两组比较差异有统计学意义(P<0.05);复发率A组5%,B组19%,两者比较差异有统计学意义(P<0.01)。结论:保妇康凝胶联合乳酸菌素阴道胶囊治疗外阴阴道假丝酵母菌病疗效确切,副作用小,复发率低。

  17. 妊娠期妇女外阴阴道假丝酵母菌检测结果与临床诊治价值的分析%Analysis on the detection results and clinical values of diagnosis and treatment of vulvovaginal candidiasis in pregnant women

    陈昌玲; 黄春琴; 张镇松; 刘秀卿

    2011-01-01

    目的:探讨妊娠期妇女外阴阴道假丝酵母菌检测结果对临床诊治的价值.方法:将2009年6月~2010年6月常规进行产前检查的孕妇,取阴道分泌物进行假丝酵母菌培养和药物敏感试验,以培养阴性者为对照组,培养阳性且有症状者为患者组,培养阳性无症状者为携带组,对3组的治疗效果及妊娠结果进行对比.结果:患者组与对照组的胎膜早破、早产、产褥感染等的发生率差异有统计学意义(P<0.05).结论:对妊娠期妇女阴道分泌物进行假丝酵母菌的培养鉴定及药物敏感性监测,为临床诊治妊娠期外阴阴道假丝酵母菌病有较大的社会、经济效益.%Objective: To explore the value of detection results of vulvovaginal eandidinsis in clinical diagnosis and treatment of pregnant women with vulvovaginal candidiasis. Methods: The vaginal secretion samples were obtained from the pregnant women receiving routine prenatal examination in the hospital from June 2009 to June 2010, then candida cultivation and drug sensitive test were carried out, the patients with negative cultivation results were selected as control group, the patients with positive cultivation results and symptoms were selected as case group, the patients with positive cultivation results and without symptoms were selected as carrier group, the curative effects and pregnancy outcomes in the three groups were compared. Results: There was significant difference in the incidences of premature rupture of membrane, premature delivery and puerperal infection between case group and control group (P < 0. 05 ) . Conclusion: Cultivation, determination and drug sensitive test of candida in vaginal secretions of pregnant women have enormous social effect and economic effect in the course of clinical diagnosis and treatment of vulvovaginal candidiasis during pregnancy.

  18. 药物联合波姆光控制重度外阴阴道假丝酵母菌病症状的疗效观察%The clinical effect of POME optical treatment in patients with severe vulvovaginal candidiasis

    石峥; 周红; 杜庆秀

    2011-01-01

    Objective To evaluate the clinical effect of local POME optical treatmeat in patients with severe vulvovaginal candidiasis (VVC) . Methods Ninety-eight patients, who were diagnosed as severe vulvovaginal candidiasis from January to December 2009 in Beijing Wuzhou Women's Hospital gynecologicalclinic,were enrolled into this study and divided into control and physiotherapy groups randomly. The patients of control group (re =48) were treated with oral Fluconazole and had Miconazde Nitrate suppositories settlement into vagina, while in the physiotherapy group (n =50) the patients accepted local POME optical treatment combined with the same medications as control. The therapeutic effect were evaluated and compared according to the subjective symptoms and VVC scores in both groups. Results The clinical symptoms of physiotherapy group were relieved within 4 hours after the treatment, which were significantly depressed compared to before the treatment (itching score 1.4 ±0.3 vs. 2. 8 ±0. 5,P 0.05). Conclusion Local POME optical treatment combined with drug treatment in severe VVC patients not only has better effect than drug treatment alone,but also relieves clinical symptoms more quickly.%目的 探讨局部波姆光理疗对控制重度外阴阴道假丝酵母菌病(VVC)症状的临床疗效.方法 将我院妇科2009年1-12月确诊的重度VVC患者98例按照不同的治疗方法 分成2组:对照组48例,用口服氟康唑和阴道放置硝酸咪康唑栓治疗;理疗组50例,用局部波姆光理疗联合对照组方法 治疗;通过对患者的主观症状和治疗后VVC分别评分进行比较,观察2组方法 的疗效.结果 理疗组患者的临床症状在治疗后4 h就快速缓解,其中瘙痒评分低于治疗前[(1.4±0.3)分与(2.8±0.5)分,P0.05).结论 局部波姆光理疗联合药物治疗重度VVC,不仅可以加强单纯药物治疗的疗效,而且能快速缓解临床急性期的症状.

  19. 克霉唑阴道片和乳酸杆菌胶囊对妊娠合并白念珠菌性阴道炎患者阴道pH值变化的影响%Influence on Changes of PH Value between Clotrimazole Vaginal Tablets and Lactobacillus Capsules for Pregnancy Infected with Vulvovaginal Candidiasis

    赖足足

    2015-01-01

    目的:对比克霉唑阴道片和乳酸杆菌胶囊治疗妊娠合并白念珠菌性阴道炎的临床疗效差异及对阴道pH值的影响。方法选取2012年1月~2014年8月我院收治的110例妊娠期白念珠菌性阴道炎患者作为研究对象,采用随机数字表法将所选患者分为克霉唑组和乳酸杆菌组,各55例,克霉唑组给予克霉唑阴道片治疗,乳酸杆菌组给予乳酸杆菌胶囊治疗,综合比较两组患者治疗总有效率、临床症状、体征改善时间及治疗前阴道pH值变化情况。结果克霉唑组治疗总有效率为96.4%,高于乳酸杆菌组的83.6%(<0.05);克霉唑组患者瘙痒、疼痛、烧灼感、白带异常、异常分泌物等临床症状、体征改善时间明显优于乳酸杆菌组(<0.05);治疗后,克霉唑组阴道pH值为(3.7±0.7),低于乳酸杆菌组的(4.0±0.5)<0.05)。结论 pH值可作为孕期白念珠菌性阴道炎的辅助诊断指标,克霉唑阴道片治疗妊娠合并白念珠菌性阴道炎效果确切,可有效降低阴道pH值,改善患者的临床症状。%Objective To compare the dif erent ef ects between clotrimazole vaginal tablets and lactobacil us capsules for pregnancy infected with vulvovaginal candidiasis and the influence on changes of pH value.Methods 110 cases of pregnancy infected with vulvovaginal candidiasis in our hospital were randomly divided into clotrimazole vaginal tablet group and lactobacil us capsule group,each were 55 cases.Clotrimazole vaginal tablet group were treated with clotrimazole vaginal tablets.Lactobacil us capsule group were treated with lactobacil us capsule.The total ef ective rate,clinical symptoms and signs improved time and changes of pH value before and after treatment were compared.Results The total ef ective rate of clotrimazole vaginal tablet group was 96.4%%,which was higher than 83.6%of lactobacil us capsule group ( <0.05).Clinical symptoms and signs of itching,pain,burning sensation

  20. Blastomycosis

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  1. Treatment for Valley Fever (Coccidioidomycosis)

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  2. Symptoms of Valley Fever (Coccidioidomycosis)

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  3. Sporotrichosis

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  4. Histoplasmosis

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  5. Histoplasmosis Risk and Prevention

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  6. Treatment and Outcomes of Histoplasmosis

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  7. Fungal Diseases

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  8. Fungal Diseases: Ringworm

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  9. Valley Fever (Coccidioidomycosis)

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  10. Fungal Diseases: Ringworm Risk & Prevention

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  11. Who Gets Fungal Infections?

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  12. Valley Fever (Coccidioidomycosis) Statistics

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  13. Risk and Prevention of Aspergillosis

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  14. Mucormycosis (Zygomycosis)

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  15. Aspergillosis

    ... Candidiasis Oropharyngeal / Esophageal Candidiasis Genital / vulvovaginal candidiasis Invasive candidiasis Definition Symptoms Risk & Prevention Sources Diagnosis Treatment Statistics Healthcare Professionals ...

  16. The risk factors of gestational vulvovaginal candidiasis and its influence on the pregnancy outcome%妊娠期外阴阴道假丝酵母菌病的危险因素及对妊娠结局的影响

    黄萍; 马小俊

    2011-01-01

    Objective To investigate the risk factors of vulvovaginal candidiasis( VVC )during pregnancy, and the influence of VVC on the pregnancy outcome. Methods A total of 800 pregnant women were enrolled in the study. All the cases underwent questionnaire survey, routine vaginal secretion examination and detection of Candida mycoderma. The pregnancy outcomes were followed up. These cases were divided into 2 groups:VVC and non-VVC group. In VVC group, the cases were divided into treatment and untreatment subgroups. Results The gravida' s bad health habits, no antenatal examination and history of VVC were closely related to the gestational VVC. In VVC group, the incidence of preterm rupture of membranes( 15.52% ), colpoperineal laceration( 8.62% ) and cesarean section ( 55.17% ) increased. The incidences of complications reduced after standardized therapy. Conclusion Gestational VVC could result in the adverse pregnancy outcomes. Education and strengthening of good hygiene habits and antenatal examination, could prevent and reduce gestational VVC. Standardized therapy for VVC could obviously improve the pregnancy outcome.%目的 探讨妊娠期外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)发病的危险因素以及对妊娠结局的影响.方法 随机选取2010-01~2010-04在我院建档并规律产检、分娩的孕妇800例,对其进行调查问卷、阴道分泌物常规检查以及妊娠结局的随访,根据有无VVC感染分为VVC组和非VVC组,在VVC组中按有无规范治疗,分为治疗组和未治疗组.通过VVC组和非VVC组的比较了解VVC的危险因素,并且分析非VVC组、治疗组和未治疗组的妊娠结局.结果 孕妇的卫生习惯不良、孕前未行产前检查、孕前曾患VVC是妊娠期VVC的危险因素,妊娠期VVC可导致胎膜早破(15.52%)、会阴阴道裂伤(8.62%)以及剖宫产率(55.17%)的升高,经规范治疗后各种并发症的发生率明显下降.结论 妊娠期VVC可能会造成不良妊娠

  17. 光动力治疗女性尖锐湿疣合并外阴阴道念珠菌病的临床疗效观察%Analysis of the efficacy of photodynamic therapy of condyloma acuminatum with vulvovaginal candidiasis of women infections

    张云杰; 杨宇光; 郭小娟; 邹先彪

    2013-01-01

    Objective To evaluate the clinical effect of 5-ALA photodynamic therapy in treating patient of condyloma acuminatum(CA) with vulvovaginal candidiasis infections.Methods The 66 cases in treatment group were treated by photodynamic combined with antifungal therapy,while the 50 cases in control group by frozen combined with antifungal therapy.Results Complete response rate of treatment group was 92.4%,while that of control group was 78.0%.The difference was statistically significant (x 2 =4.978,P =0.026) ; The cure rate of fungal infection of treatment group was 97.0%,while that of control group 86.0%.The difference was statistically significant (x 2 =4.783,P =0.029).Patients were followed up for 6 months,the recurrence rate of condyloma of treatment group was 7.5%,the control group 24.0% (x 2 =6.136,P =0.013).After 2 months of follow-up,the recurrence rate of fungi infection of treatment group was 4.5%,whlie the control group 16.0% (x 2 =4.349,P =0.037).Conclusion 5-ALA photodynamic combined with antifungal is a safe and effective treatment of condyloma acuminatum (CA) with vulvovaginal candidiasis with low recurrence rate.%目的 评价5-氨基酮戊酸光动力治疗尖锐湿疣合并外阴阴道念珠菌病的临床疗效.方法 治疗组66例患者采用光动力联合氟康唑胶囊和克霉唑栓,对照组50例患者采用冷冻联合氟康唑胶囊和克霉唑栓.结果 治疗组尖锐湿疣完全反应率92.4%,对照组尖锐湿疣完全反应率78.0%,差异有统计学意义(x2=4.978,P=0.026).治疗组真菌感染治愈率97.0%,对照组真菌感染治愈率86.0%,差异有统计学意义(x2=4.783,P=0.029).随访6个月,治疗组尖锐湿疣复发率7.5%,对照组复发率24.0%,两组间差异有统计学意义(2=6.136,P=0.013).随访2个月后复查真菌,治疗组真菌复发率4.5%,对照组复发率16.0%,治疗组复发率显著低于对照组,差异有统计学意义(x2 =4.349,P=0.037).结论 5-氨基酮戊酸光动力联

  18. The analysis of Clinical effects on Treating Recurrent vulvovaginal candidiasis, RVVC with Traditional Chinese and Western Medicine%中西医结合治疗复发性外阴阴道假丝酵母菌病69例临床疗效分析

    陈亮香; 王冬莲; 廖湘玲

    2013-01-01

    目的:探讨中西药联合治疗妇女复发性外阴阴道假丝酵母菌病的疗效。方法:将69例复发性外阴阴道假丝酵母菌病患者随机分为3组。A组23例:口服抗真菌药氟康唑并阴道上制霉菌素栓(我院自制制剂);B组23例:在A组治疗上,洗液改用阴炎净洗液(我院自制制剂);C组23例:在B组治疗上加服中药汤剂龙胆泻肝汤加味连续10天。结果:A组总有效率52.1%,B组总有效率69.6%, C组的总有效率91.3%, B组有效率高于A组,但组间差异不具有统计学意义, X2=1.889,p>0.05;C组有效率高于B组,差异具有统计学意义,X2=6.036,p0.05). Conclusion:The traditional Chinese and western medicine are effective method to recurrent vulvovaginal candidiasis.

  19. 中西医结合治疗复发性外阴阴道假丝酵母菌病的临床观察%Clinical Observation on the Treatment of Recurrent Vulvovaginal Candidi-asis with Combination of TCM and Western Medicine

    王金凤; 刘云波

    2015-01-01

    Objective: To observe the clinical curative effect of combined traditional Chinese medicine (TCM) and western medicine on recurrent vulvovaginal candidiasis (RVVC). Methods: Seventy-one patients with RVVC were randomly divided into observation group (n=36) and control group (n=35), patients in the control group were treated with fluconazole combined nifuratel nysfungin vaginal soft capsules; based on the control group treatmemt, patients of the observation group received syndrome differentiated decoctions of Chinese medicine for fumigation/wash and oral administration. Results: The observation group showed higher cure rate and lower relapse rate than the control group, the differences were statistically significant (P<0.05). Conclusion: The combined treatment of TCM and western medicine has better curative effects for RVVC.%目的:中西医结合治疗复发性外阴阴道假丝酵母菌病(RVVC)的临床疗效。方法:71例RVVC患者随机分为观察组(n=36)和对照组(n=35);对照组给予氟康唑联合硝呋太尔制霉素阴道软胶囊;观察组在对照组用药基础上,加中药煎汤熏洗和内服。结果:观察组治愈率高,复发率低,明显优于对照组,差异有统计学意义(P<0.05)。结论:中西医结合治疗RVVC疗效好。

  20. 氟康唑联合定君生治疗复发性外阴阴道假丝酵母菌病102例%102 cases of recurrent vulvovaginal candidiasis treated with fluconazole combined with living preparation of lactobacillus

    黄志娟

    2014-01-01

    Objective:To observe the clinical efficiency of treating recurrent vulvovaginal candidiasis using fluconazole combined with Living Preparation of Lactobacillus. Methods: All of the 204 patients who suffered from recurrent vulvovaginal candidiasis between September 2011 and September 2013 were randomly divided into the treatment group(102) and the control group(102). Patients in the treatment group took fluconazole 150mg/ d on the 2nd and 4th day of menstruation and inserted Living Preparation of Lactobacillus into their vaginas on the 10th day after menstruation while patients in the control group only inserted Living Preparation of Lactobacillus into their vaginas on the 10th day after menstruation, and continuously for 3 menstrual cycles. Consequently, we observed the effectiveness immediately after the end of treatment and one month later.Results:Of the 102 cases in the treatment group, 90 were cured, 10 showed improvement and only 2 were ineffective, and thus the total effective rate reached 98.04%. In the control group, 72 cases were cured, 18 cases showed improvement and 12 cases were ineffective, and the total effective rate was 88.24%. Compare the total efficiency of the two groups and we find out that the difference is statistically significant (P<0.05). One month after the completion of treatment, reexamination found 6 cases of recurrence in the treatment group and the recurrence rate was 6%; in the control group, there were 13 cases of recurrence and the recurrence rate was 14.44%. The comparison between the two groups shows the difference is statistically significant ( P < 0. 05). Conclusion: The treatment of recurrent vulvovaginal candidiasis using fluconazole combined with living preparation of lactobacillus is remarkably effective and consequently is worthy of popularization.%目的:观察氟康唑联合定君生治疗复发性外阴阴道假丝酵母菌病的临床疗效。方法:将2011年9月至2013年9月收治的复发性外阴阴道假丝

  1. Studies of the Clinical Effect for Recurrent Vulvovaginal Candidiasis by Using Coptis Chinensis Decoftion Combined with Fluconazole%黄连联合氟康唑治疗复发性外阴阴道假丝酵母菌病临床观察

    应基; 李瑞兰

    2014-01-01

    目的:探讨单味黄连煎剂联合氟康唑治疗复发性外阴阴道假丝酵母菌病的临床疗效。方法选取2011年6月~2013年6月妇科门诊RVVC患者112例,随机分为两组。观察组60例,单味煎剂阴道给药联合氟康唑口服初始治疗,初始治疗有效者单味黄连煎剂阴道给药维持治疗;对照组62例,克霉唑栓阴道用药联合氟康唑口服初始治疗,初始治疗有效者克霉唑栓阴道给药维持治疗。结果①观察组治愈率(73.34%)低于对照组(75.80%),差异无统计学意义(>0.05);观察组总有效率(91.67%)高于对照组(90.32%),差异无统计学意义(>0.05)。②治疗结束3个月观察组复发率(8.33%)低于对照组(11.29%),差异无统计学意义(>0.05);治疗结束6个月观察组复发率(11.67%)显著低于对照组(25.81%),差异有统计学意义(0.05). the total ef ective rate of observation group (91.67%) was higher than control group (90.32%), the dif erence was not statistically significant ( >0.05). ②Within three months, the recurrence rate of observation group (8.33%) was lower than control group (11.29%),the dif erence was not statistically significant ( >0.05). Within six months , the recurrence rate of observation group(11.67%) was lower than control group(25.81%),the dif erence was statistical y significant ( <0.05). Conclusion Single coptis chinensis decoftion combined with fluconazole on recurrent vulvovaginal candidiasis is recommendable therapy for its satisfactory ef icacy, mainly in control ing forward recurrence rate.

  2. Urinoma and arterial hypertension complicating neonatal renal candidiasis

    During antibiotic treatment for E.coli urinary tract infection and meningitis, a male new born developed a Candida albicans urinary tract infection with a mycotic kidney abcess and pelvicalyceal fungus balls diagnosed by US investigations and confirmed by radiology. Three weeks later a perirenal urinoma with arterial hypertension developed. After surgical treatment of the urinoma the arterial pressure returned to normal. (orig.)

  3. Tuberculosis of gallbladder with candidiasis, a rare entity.

    Leong, B D K; Chan, K W; Ramu, P; Kumar, V M; Chuah, J A

    2011-06-01

    Gallbladder tuberculosis (GT) is an extremely rare condition. This is thought to be due to the protective property of bile against the infection. Clinical and radiological diagnosis of GT is difficult. We describe a case of GT who initially presented to us with jaundice, a right hypochondrial mass and computed tomographic findings suggestive of gallbladder empyema. Diagnosis was made from histopathological examination of the resected gallbladder which revealed epitheloid granulomas with caseating necrosis and presence of Langhan's giant cells. From a literature search and to the best of our knowledge, this is the first GT to be reported in South East Asia. PMID:22106698

  4. Immunopathogenesis of Oropharyngeal Candidiasis in Human Immunodeficiency Virus Infection

    de Repentigny, Louis; Lewandowski, Daniel; Jolicoeur, Paul

    2004-01-01

    Oropharyngeal and esophageal candidiases remain significant causes of morbidity in human immunodeficiency virus (HIV)-infected patients, despite the dramatic ability of antiretroviral therapy to reconstitute immunity. Notable advances have been achieved in understanding, at the molecular level, the relationships between the progression of HIV infection, the acquisition, maintenance, and clonality of oral candidal populations, and the emergence of antifungal resistance. However, the critical i...

  5. Urinoma and arterial hypertension complicating neonatal renal candidiasis

    Sirinelli, D.; Schmit, P.; Biriotti, V.; Bensman, A.; Lupold, M.

    1987-02-01

    During antibiotic treatment for E.coli urinary tract infection and meningitis, a male new born developed a Candida albicans urinary tract infection with a mycotic kidney abcess and pelvicalyceal fungus balls diagnosed by US investigations and confirmed by radiology. Three weeks later a perirenal urinoma with arterial hypertension developed. After surgical treatment of the urinoma the arterial pressure returned to normal.

  6. 乳杆菌活菌胶囊配合制霉菌素治疗外阴阴道假丝酵母菌病的临床研究%Clinical observation on Living Preparation of Lactobacillus combined with Nystatin in the treatment of vulvovaginal candidiasis

    高卫辉

    2011-01-01

    Objective: To investigate the efficacy of Living Preparation of Lactobacillus combined with Nystatin in the treatment of vulvovaginal candidiasis (VVC). Methods: 120 patients with VVC were randomly divided into two groups, treatment group and control group, with 60 cases in each group. Treatment group was given Nystatin (500 000 U/tablet), one tablet, once a day, going up through vagina on medicine, for 10 days, and then given Living Preparation of Lactobacillus, one tablet, going up through vagina on medicine, once a day, for 5 days. Using the same ways after menstrual in the next two months. Control group was given Nystatin (500 000 U/tablet), one tablet, going up through vagina on medicine, once a day, for 10 days, using the same ways after menstrual in the next two months. The efficacy of two groups were evaluated after 3 courses of treatment. Results: The total effective rate was 96.7% in the control group, while 73.3% in the treatment group. Comparison of the effective in the two groups, the treatment group had a better efficacy than the control group, there was significant difference between two groups (ρ<0.05). Conclusion: Living Preparation of Lactobacillus combined with Nystatin in the treatment of VVC has a remarkable efficacy.%目的:探讨乳杆菌活菌胶囊(商品名:定君生)配合制霉菌素治疗外阴阴道假丝酵母菌病(VVC)的疗效.方法:把120例VVC患者随机分为治疗组(60例)和对照组(60例).治疗组先给予制霉菌素(50万 U/片)治疗,阴道上药,1片/次,1次/ d,连续用药10 d,接着给予定君生1粒,阴道上药,1次/ d,连续5 d;第2、3个月月经干净后继续给予制霉菌素、定君生,阴道上药,方法和剂量同前.对照组仅用制霉菌素(50万 U/片)1片,阴道上药,1次/ d,连续10 d;第2、3个月月经干净后继续给予制霉菌素,阴道上药,方法和剂量同前.两组治疗3个疗程后观察其疗效.结果:治疗组总有效率为96.7%,对照组总有效率为73.3%,治疗

  7. 止痒洗剂联合妇炎消生物敷料栓治疗妊娠合并外阴阴道假丝酵母菌病的临床观察%Clinical observation on the effect of anti -itching lotion combined with Fuyanxiao biological dressing supposi-tory in treatment of pregnancy and vulvovaginal candidiasis

    康玉惠

    2015-01-01

    目的:观察止痒洗剂联合妇炎消生物敷料栓治疗妊娠合并外阴阴道假丝酵母菌病(VVC)的临床疗效及复发率。方法选择妊娠合并 VVC 患者236例,将患者以随机数字表法分为两组:治疗组、对照组,各118例。治疗组采用止痒洗剂联合妇炎消生物敷料栓治疗,对照组单独采用妇炎消生物敷料栓治疗,疗程结束后比较两组的临床疗效及复发率。结果治疗组治愈率90.68%,高于对照组的57.62(χ2=10.731,P <0.01);治疗组复发率3.39%,低于对照组的13.56%,差异有统计学意义(χ2=10.733,P <0.05)。结论止痒洗剂联合妇炎消生物敷料栓治疗妊娠合并 VVC 可有效改善患者的临床症状,降低复发率。%Objective To observe the effect of antipruritic lotion combined Fuyanxiao with biological dress-ing suppository in the treatment of vulvovaginal candidiasis in pregnancy(VVC)the clinical curative effect and the recurrence rate.Methods 236 patients with pregnancy complicated with VVC,would be in community were randomly divided into the two groups:combination treatment group,single drug group,118 cases in each.Combination therapy using antipruritic lotion combined Fuyanxiao biological dressing suppository combined treatment,the control group was treated by Fuyanxiao biological dressing suppository treatment,two groups of clinical efficacy and recurrence rate after the end of treatment were compared.Results The cure rate in the combined treatment group was 90.68%,which was higher than 57.62 % in the control group(χ2 =10.731,P <0.01);The recurrence rate in the combined treatment group was 3.39%,which was lower than 13.56% in the control group(χ2 =10.733,P <0.05).Conclusion The antipruritic lotion combined Fuyanxiao biological dressing suppository in the treatment of pregnancy with VVC can effectively improve the clinical symptoms of patients,reduce the recurrence rate.

  8. 单味黄连煎剂治疗外阴阴道假丝酵母菌阴道病81例疗效观察%Curative effect of single coptis chinensis decoftion on treatment of 81 cases of vulvovaginal candidiasis

    杨懿; 李瑞兰; 宁玉梅; 叶慧君; 余姬文; 赵蕾

    2013-01-01

    目的 探讨单味黄连煎剂治疗外阴阴道假丝酵母菌病(VVC)的临床疗效以及克霉唑治疗VVC的优势.方法 选择65例VVC和16例复发性外阴阴道假丝酵母菌病(RVVC)患者使用黄连煎剂为治疗组,同时选取62例VVC患者及14例RVVC患者使用克霉唑为对照组,观察两种药物对于VVC的疗效.结果 治疗组治愈显效率为97.53%,对照组为98.68%,治疗组较对照组治愈显效率低,经x2检验,差异无统计学意义;治疗组1月内检出率为7.38%,3个月复发率为7.40%,对照组1个月内检出率为15.00%,3个月复发率为25.00%,经x2检验,差异有统计学意义(P<0.05).结论 单味黄连煎剂治疗VVC的临床疗效基本等同于克霉唑,在控制其远期复发率方面具有优势.%OBJECTIVE To investigate the clinical curative effect of single coptis chinensis decoftion on treatment of vulvovaginal candidiasis (VVC)and to explore the advantages of clotrimazole in treatment of VVC.METHODS A total of 65 VVC patients and 16 recurrent vulvovaginal candidias (RVVC) patients who were treated with single coptis chinensis decoftion were selected as the treatment group,meanwhile,62 VVC patients and 14 RVVC patients who were treated with the clotrimazole were chosen as the control group.The curative effects of the two drugs on treatment of VVC were observed.RESULTS The markedly effective rate of treatment group was 97.53%,lower than 98.68% of the control group,the chi-square test demonstrated that the difference was not statistically significant.Within one month,the detection rate of the treatment group was 7.38%,and the recurrence rate was 7.40% within 3 months,while the detection rate of the control group was 15.00% within one month,and the reoccurrence rate was 25.00% within three months,the chi-square test showed that the differences were statistically significant (P< 0.05).CONCLUSION The single coptis chinensis decoftion can achieve the clinical curative effect

  9. 盐酸特比萘芬联合氟康唑治疗反复发作念珠菌性阴道炎的疗效和安全性%Therapeutic effects and safety of ternbinafine vaginal effervescent tablets combined with fluconazole in patients with recurrent vulvovaginal candidiasis

    方洁; 王心; 尚丽新; 刘敬超

    2015-01-01

    目的:观察盐酸特比萘芬联合氟康唑治疗反复发作的念珠菌性阴道炎( recurrent vulvovaginal candidasis ,RVVC)的疗效和安全性。方法228例RVVC患者随机分为氟康唑组(氟康唑150 mg,口服,1次/3 d,共3次)和联合用药组(在氟康唑组的基础上加用盐酸特比萘芬阴道泡腾片,每晚1片放入阴道,50 mg/片,连续应用2周)。观察治疗结束后两组治愈率、临床症状、体征改变、不良反应情况。结果氟康唑组治愈率为81.6%,联合用药组治愈率为92.1%,联合用药组高于氟康唑组,差异有统计学意义(χ2=5.53,P=0.02)。两组治疗后外阴灼痛、外阴瘙痒、阴道分泌物、阴道充血等各项临床症状体征均较治疗前有明显好转。联合用药组阴道分泌物、阴道充血等临床体征较氟康唑组明显减轻,差异有统计学意义(P<0.05)。两组不良反应发生率(氟康唑组13.2%,联合用药组14.9%)比较,差异无统计学意义。结论盐酸特比萘芬阴道泡腾片联合氟康唑较单独采用氟康唑治疗RVVC有更高的治愈率,且未增加不良反应。%Objective To study the efficacy and safety of ternbinafine vaginal effervescent tablets combined with fluconazole in the treatment of recurrent vulvovaginal candidiasis ( RVVC) .Methods Totally 228 patients with RVVC were randomly divided into the control group and the study group with 114 cases in each .The control group was treated with fluconazole 150 mg orally for a total of 3 times per 3 days.The study group was given the same drug as the control group and additionally treated with ternbinafine vaginal ef -fervescent tablets 50 mg every day (the treatment course was 14 days).The clinical effect, effective rate and the incidence of adverse reactions in the two groups were compared .Results The total cure rate in the study group was 92.1%, higher than that in the control group ( 81

  10. Clinical Study of Synergistic Effect of Xianglian Suppository Combined with Miconazole Suppository in Treating Simple Vulvovaginal Candidiasis%香莲栓联合咪康唑栓对单纯性外阴阴道念珠菌病增效作用研究

    吴盘红; 范瑞强; 陈信生; 谢婷

    2015-01-01

    【目的】观察香莲栓和咪康唑栓联合治疗单纯性外阴阴道念珠菌病(VVC)的抗菌增效作用,探讨中西医结合治疗外阴阴道念珠菌病的优势。【方法】将65例患者随机分为试验组35例和对照组30例。试验组给予香莲栓和咪康唑栓治疗,对照组给予咪康唑栓治疗,共用药7 d。观察2组治疗前后证候积分的变化情况,评价2组的临床疗效及安全性。【结果】(1)经1个疗程治疗后,试验组的愈显率和总有效率分别为60.00%、88.57%,优于对照组的23.33%、60.00%(均P<0.01)。(2)治疗后,2组的证候积分均较治疗前显著降低(P<0.01),且试验组在改善证候积分方面作用优于对照组(P<0.01)。(3)安全性方面:试验组中有1例诉使用后自觉外阴瘙痒加重,检查发现外阴阴道潮红,无肿,无水疱,无其他不适,嘱继续用药后患者症状消失,不考虑为过敏反应;对照组无1例出现过敏等不适表现。【结论】香莲栓和咪康唑栓联合治疗单纯性外阴阴道念珠菌病,可明显改善临床症状,且安全性好,值得在临床推广应用。%Objective To observe the antibacterial and synergistic effect of Xianglian Suppository with miconazole suppository for the treatment of simple vulvovaginal candidiasis ( VVC) , and to explore the advantages of integrative Chinese and western medicine for the treatment of simple VVC. Methods According to the principle of randomized trial, 65 simple VVC patients were divided into two groups, 35 cases in test group and 30 in control group. The test group was given Xianglian suppository and miconazole suppository, and the control group was treated with miconazole suppository, the medication lasting for 7 days. The changes of symptom scores before and after treatment were observed, and the therapeutic effect and safety were also evaluated in both groups. Results ( 1) After treatment for

  11. Functional analysis of the receptor Dectin-1 on dendritic cells from a patient with recurrent vulvovaginal candidiasis%复发性外阴阴道念珠菌病患者树突细胞Dectin-1受体的功能研究

    穰真; 崔凡; 李薇; 王有为

    2015-01-01

    目的 对比复发性外阴阴道念珠菌病(RVVC)患者和健康女性树突细胞(DC)表面Dectin-1受体信号传导及功能的差异,分析患者病情反复发作的可能原因.方法 提取1例RVVC患者和1例健康女性的单核细胞,诱导分化为DC.DC与白念珠菌共培养后,流式细胞仪测定DC表面CD83、CD86和CD80表达水平,分析细胞的成熟率;Western印迹法测定DC的Dectin-1、酪氨酸激酶(Syk)和CARD9蛋白的表达;EHSA法测定DC分泌白细胞介素23(IL-23)、肿瘤坏死因子α(TNF-α)和IL-12水平.结果 与健康人DC相比,与白念珠菌共培养24 h后,该RVVC患者DC表面CD83、CD86和CD80的表达活化不明显.与健康人DC相比,与白念珠菌共培养2h后,RVVC患者DC表达的Dectin-1没有显著性差异,但是磷酸化Syk和CARD9活化障碍.与健康人DC相比,与白念珠菌共培养6h后,RVVC患者DC分泌的IL-23、TNF-α和IL-12升高也不明显.抗人Dectin-1抗体对RVVC患者DC的Syk依赖的信号传导通路和上述细胞因子的分泌都没有进一步抑制作用.结论 该RVVC患者DC的Dectin-1受体信号传导通路障碍,导致DC成熟率降低,分泌的IL-23、TNF-α和IL-12降低,使得宿主黏膜抗念珠菌感染的天然免疫功能缺陷.%Objective To compare the Dectin-1 signal transduction pathway and its function on dendritic cells between a female patient with recurrent vulvovaginal candidiasis (RVVC) and a healthy woman,and to explore the possible mechanism for VVC recurrence in this patient.Methods Venous blood samples were collected from a female patient with RVVC and a healthy woman.Then,monocytes were isolated from the blood samples,and were induced to differentiate into dendritic cells (DCs) in vitro.The obtained DCs were divided into three groups to be cultured alone,cocultured with Candida albicans or the combination of Candida albicans and anti-Dectin-1 antibodies for different durations.Flow cytometry was performed to determine the expression levels of CD83

  12. Correlação entre os resultados laboratoriais e os sinais e sintomas clínicos das pacientes com candidíase vulvovaginal e relevância dos parceiros sexuais na manutenção da infecção em São Paulo, Brasil Relationship of laboratory results with clinical signs and symptoms of patients with vulvovaginal candidiasis and the significance of the sexual partners for the maintenance of the infection

    Humberto Fabio Boatto

    2007-02-01

    reservatórios de Candida spp e estar relacionados à manutenção da candidíase vulvovaginal.PURPOSE: to relate yeasts identified by laboratory tests to clinical signs and symptoms in patients with vulvovaginal candidiasis, and to investigate the importance of the sexual partners in the recurrence of the infection. METHODS: from July 2001 to July 2003, a sample of 179 patients aged from 18 to 65 years old, with clinical suspicion of fungal vaginitis were analyzed in a prospective study in Great São Paulo. Exclusion criteria included: pregnancy, impaired intrinsic or extrinsic immune response (including Aids, diabetes or immunosuppression; patients undergoing corticosteroid, antibiotic or hormone therapy, in post menopause, with intrauterine device (IUD or making use of vaginal douches or spemicides. Samples of vaginal and penis secretions from partners of patients with relapse of vaginitis episodes were collected for microscopy and fungal culture. Fungal colonies isolated in CHROMagar Candida were identified by classical methods. Fisher's exact t-test was used to correlate the clinical picture with the yeasts isolated from patients. RESULTS: the most relevant clinical signs and symptoms were pruritus and vaginal discharge followed by erythema and edema, statistically independent from the etiological agent. Direct microscopy revealed yeasts in 77 patients with vulvovaginitis, and 40 Candida spp cultures were obtained. Candida albicans (70%, C. glabrata (20%, C. tropicalis (7,5% and C. guilliermondii (2,5% were identified. The yeasts prevalent in partners were C. albicans and C. glabrata. The same species were detected in female and male sex partners in 87% of the cases. CONCLUSIONS: fungal vulvovaginitis was more frequent in women between 18 and 34 years old. No correlation was observed between the species of yeast detected and clinical symptomatology. Sexual partners are important Candida spp reservoirs and may be related to the maintenance of the vulvovaginal

  13. Immunodiagnosis of opportunistic mycoses: detection of fungal antigenemia by radioimmunoassays in systemic candidiasis and aspergillosis

    The authors have developed radioimmunoassays to the Candida carbohydrate, mannan, and to an Aspergillus cell wall carbohydrate. They evaluate these radioimmunoassays with sera from rabbit models of disseminated mycoses, and further evaluate the radioimmunoassays for their diagnostic usefulness in a panel of patient sera. (Auth.)

  14. Role of tetracycline in pathogenesis of chronic candidiasis of rat tongues.

    Allen, C M; Beck, F. M.; Lurie, F A; Pinsky, H M

    1985-01-01

    Forty female Sprague-Dawley rats were randomly divided into two groups. Group 1 animals received tetracycline solution (0.1% during week 1 and 0.01% thereafter) as drinking water, as in previous studies. Group 2 animals received double-distilled demineralized water. Animals in both groups were inoculated orally with an equal number of viable, mucosally pathogenic Candida albicans organisms. After 20 weeks, inspection of the tongues showed gross lesions in 16 of the 20 animals in group 1 and 1...

  15. Candidiasis sistémica en un feto de 12 semanas de edad gestacional

    Sánchez Baracaldo, Lilia María; Müller, Edith Ángel

    2011-01-01

    Presentamos el caso de un feto de 12 semanas de cdad gestacional en el cual el estudio de anatomia patológica documento un proceso sistémico por Candida con compromiso de la piel y los pulmones. Llamamos la atencton sobre la posibilidad que tiene el feto de adquirir este tipo de lnfección durante el embarazo, ya que las vulvovaginitis por Candida son frecuentes durante este periodo. Igualmente destacamos la importancia del examen anatomopatclógico que nos permitió lIegar al diagnóstico de est...

  16. Vulvovaginal candidiasis in Mato Grosso, Brazil: pregnancy status, causative species and drugs tests

    Luciana Basili Dias

    2011-12-01

    Full Text Available Causative agent in majority of VVC is Candida albicans, but infection due to non-C. albicans is common. Use of empiric antifungal therapy in Brazil due to syndromic management of vulvovaginitis could act as risk factor for increase resistance among VVC causative agents. From Mato Grosso patients, 160 with culture-proved among 404 women who had clinical symptoms of VVC, were enrolled in this study. 70 non-pregnant women and 90 pregnant women were included. Candida albicans was the most prevalent, representing 72.9% in the non-pregnant group and 92.3% in the pregnant group. Differences in species distribution were noted between the two groups, being C. parapsilosis the second more prevalent species among non-pregnant women. Susceptibility testing revealed high susceptibility to fluconazole (except for C. krusei, itraconazole, ketoconazole, and amphotericin B regardless the species (C. albicans, C. parapsilosis, C. tropicalis, C. glabrata, C. krusei analyzed.

  17. Emergence of Azoles Resistance Candida species in Iranian AIDS defined patients with oropharyngeal candidiasis

    Farzad Katiraee

    2015-09-01

    Conclusion: Based on the findings, it can be concluded that screening of resistant Candida isolates by disk diffusion or broth dilution method is essential for the surveillance and prevention of antifungal resistance in patient management. Although nystatin is widely used in clinical practice for HIV patients in Iran, no evidence of enhanced resistance against this agent was found on the other hand, resistance to azole antifungals, particularly fluconazole, increased. Considering the lack of resistance to caspofungin, administration of this agent is suggested for the treatment of OPC in AIDS patients.

  18. A 16-year-old boy with emphysematous gastritis and oesophageal candidiasis.

    Yusef, Daniel; Waran, Ariane; Vamvakiti, Ekaterini

    2014-01-01

    Emphysematous gastritis is a rare and frequently fatal condition caused by invasion of gas-forming bacteria into the gastric wall. There have only been a handful of reported cases in the paediatric population, and none of these have evidence of candidal infection or mucormycosis. Patients typically present with abdominal pain, vomiting, malaena and haematemesis. Risk factors for emphysematous gastritis are those that interfere with the natural barriers to infection in the stomach. Diagnosis is made on the basis of typical appearances on abdominal CT. Treatment is generally conservative with surgery reserved for failed medical management or later complications. Antimicrobial cover should be broad with a low threshold for antifungals. It is important to look for predisposing factors for this condition, perhaps including an assessment of the patient's immunocompetency. We present a 16-year-old boy with global developmental delay who presented with this condition associated with candidal infection. PMID:25213783

  19. A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient

    Mohsen Ashrafi

    2015-01-01

    Results: From 2009 to 2011, 72 patients with hematologic malignancies and bone marrow transplant recipients were evaluated for IC. The female to male ratio was 27:45; the mean age was 32.1 years. The most common malignancy in this patient was acute myeloid leukemia (AML (27.8% and acute lymphoblastic leukemia (ALL (26.4%. Out of 72 patients, 11 patients (15.3% had positive real time PCR /probe results. Based on the melting temperature (Tm analysis, 5 (45.4% C. krusei, 3 (27.2% C. tropicalis, 2 (18.1% C. parapsilosis and 1 C. albicans (9% were identified. According to the revised EORTC / MSG, 1 patient (9% and 10 patients (91% were defined as proven and possible groups of IC, respectively. The mortality rate in proven and possible IC patient was found 54.5%. Conclusion: The established Real-time PCR/FRET probe assay is an appropriate diagnostic tool for the detection of Candida species DNA and the management of patients suffering from hematologic malignancies and bone marrow recipient are at risk for IC.

  20. Interferon-gamma Immunotherapy in a Patient With Refractory Disseminated Candidiasis.

    Buddingh, Emilie P; Leentjens, Jenneke; van der Lugt, Jasper; Dik, Willem A; Gresnigt, Mark S; Netea, Mihai G; Pickkers, Peter; Driessen, Gertjan J

    2015-12-01

    Despite advances in supportive care and novel antifungal agents, mortality caused by invasive Candida infection is high. A 3-year-old boy with disseminated Candida dubliniensis infection during induction chemotherapy for acute lymphoblastic leukemia deteriorated despite resolution of neutropenia and appropriate antifungal treatment. Monocyte human leukocyte antigen-DR expression was extremely low, suggesting immunoparalysis. Adjuvant immunotherapy with interferon-gamma restored the immune response, which was accompanied by clinical and radiographic recovery. PMID:26379166

  1. Sonographic detection of intrarenal and intraarterial fungus balls in a preterm infant due to systemic candidiasis

    Shortly after birth a preterm infant suffering from aspiration syndrome and subsequent Pseudomonas aeruginosa sepsis showed signs of renal insufficiency and mycotic infection: Yeast cells were identified in several urinalyses; there was also an increasing anti-Candida IgM antibody titer. At the same time sonographic examinations revealed an increasing echogenicity of the renal cortex and echogenic masses of variable size which did not cause acoustic shadows in both enlarged kidneys. A few days later, we found a right-sided hydronephrosis caused by an intraureteric prevesical mass of equal echogenicity. As we could observe sonographically, the aggressive antimycotic therapy was successful. Eleven weeks later there were signs of cardiac insufficiency. An angiographically demonstrated filling defect within the pulmonary artery showed the same sonographic findings as the previously found intrarenal masses. The baby underwent embolectomy and recovered. The thrombotic material contained yeast cells giving evidence of systemic Candidasis. Provided appropriate equipment is available, ultrasound today is an excellent non-invasive screening and followup method not only for echoencephalography, but also for more complicated neonatologic problems as seen here. The detailed observation of a changing echogenicity of the renal cortex and pelvis is important and often allows a decisive diagnostic clue before other radiological methods become conclusive. (orig.)

  2. In-111 labeled leukocyte scintigraphy in a case of multifocal candidiasis

    The value of indium-111 labeled leukocyte scintigraphy for the diagnosis of infection in the general population is well documented; there is less information available on its role in the evaluation of the immunocompromised patient. In this study, leukocyte scintigraphy was performed on a 31-year-old immunocompromised woman who had a possible intra-abdominal abscess. No abscess was detected, but intense oral, esophageal, gastric, and vaginal uptake was observed. Candida infection was histologically confirmed at all four sites

  3. In Vivo Pharmacokinetics and Pharmacodynamics of a New Triazole, Voriconazole, in a Murine Candidiasis Model

    Andes, D.; Marchillo, K.; Stamstad, T.; Conklin, R.

    2003-01-01

    In vivo studies have described the pharmacodynamic (PD) characteristics of several triazoles. These investigations have demonstrated that the 24-h area under the concentration-time curve (AUC)/MIC ratio is the critical pharmacokinetic (PK)-PD parameter associated with treatment efficacy. Further analyses from these in vivo studies have demonstrated that a triazole free drug 24-h AUC/MIC of 20 to 25 is predictive of treatment success. We used a neutropenic murine model of disseminated Candida ...

  4. In vivo pharmacokinetics and pharmacodynamics of a new triazole, voriconazole, in a murine candidiasis model.

    Andes, D; Marchillo, K; Stamstad, T; Conklin, R

    2003-10-01

    In vivo studies have described the pharmacodynamic (PD) characteristics of several triazoles. These investigations have demonstrated that the 24-h area under the concentration-time curve (AUC)/MIC ratio is the critical pharmacokinetic (PK)-PD parameter associated with treatment efficacy. Further analyses from these in vivo studies have demonstrated that a triazole free drug 24-h AUC/MIC of 20 to 25 is predictive of treatment success. We used a neutropenic murine model of disseminated Candida albicans infection to similarly characterize the PK-PD of the new triazole voriconazole. PK and PD parameters (percentage of time that the concentration remains above the MIC [T > MIC], AUC/MIC ratio, and peak level in serum/MIC ratio) were correlated with in vivo efficacy, as measured by the organism number in kidney cultures after 24 h of therapy. Voriconazole kinetics and protein binding were studied in infected neutropenic mice. Peak level/dose and AUC/dose values ranged from 0.1 to 0.2 and 0.1 to 0.7, respectively. The serum elimination half-life ranged from 0.7 to 2.9 h. The level of protein binding in mouse serum was 78%. Treatment efficacy with the four dosing intervals studied was similar, supporting the AUC/MIC ratio as the PK-PD parameter predictive of efficacy. Nonlinear regression analysis also suggested that the AUC/MIC ratio was strongly predictive of treatment outcomes (R(2) for AUC/MIC ratio = 82%, R(2) for peak level/MIC ratio = 63%, R(2) for T > MIC = 75%). Similar studies were conducted with nine additional C. albicans isolates with various voriconazole susceptibilities (MICs, 0.007 to 0.25 micro g/ml) to determine if a similar 24-h AUC/MIC ratio was associated with efficacy. The voriconazole free drug AUC/MIC ratios were similar for all of the organisms studied (range, 11 to 58; mean +/- standard deviation, 24 +/- 17 [P = 0.45]). These AUC/MIC ratios observed for free drug are similar to those observed for other triazoles in this model. PMID:14506026

  5. Is candidiasis the true cause of vulvovaginal irritation in women with diabetes mellitus?

    Rowe, B. R.; Logan, M N; Farrell, I; Barnett, A H

    1990-01-01

    Vulvovaginitis is common in diabetic women and is often treated with antifungal agents on the assumption that the causative organism is Candida albicans. In a survey of 100 consecutive diabetic women attending a diabetes clinic 36 had complained to their general practitioner about vulvovaginal irritation during the past three years and 26 were treated with antifungal agents without a vaginal examination or swabs being taken. In a separate study 27 post-menopausal women with non-insulin depend...

  6. In Vivo Analysis of Secreted Aspartyl Proteinase Expression in Human Oral Candidiasis

    Naglik, Julian R.; Newport, George; White, Theodore C.; Fernandes-Naglik, Lynette L.; Greenspan, John S.; Greenspan, Deborah; Sweet, Simon P.; Challacombe, Stephen J; Agabian, Nina

    1999-01-01

    Secreted aspartyl proteinases are putative virulence factors in Candida infections. Candida albicans possesses at least nine members of a SAP gene family, all of which have been sequenced. Although the expression of the SAP genes has been extensively characterized under laboratory growth conditions, no studies have analyzed in detail the in vivo expression of these proteinases in human oral colonization and infection. We have developed a reliable and sensitive procedure to detect C. albicans ...

  7. In-111 labeled leukocyte scintigraphy in a case of multifocal candidiasis

    Palestro, C.J.; Vega, A.; Kim, C.K.; Goldsmith, S.J. (Mount Sinai School of Medicine, New York, NY (USA))

    1990-06-01

    The value of indium-111 labeled leukocyte scintigraphy for the diagnosis of infection in the general population is well documented; there is less information available on its role in the evaluation of the immunocompromised patient. In this study, leukocyte scintigraphy was performed on a 31-year-old immunocompromised woman who had a possible intra-abdominal abscess. No abscess was detected, but intense oral, esophageal, gastric, and vaginal uptake was observed. Candida infection was histologically confirmed at all four sites.

  8. Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis

    Torey, Angeline; Vijayarathna, Soundararajan; Subramanion L. Jothy; Gothai, Sivapragasam; Chen, Yeng; Latha, Lachimanan Yoga; Kanwar, Jagat R; Dharmaraj, Saravanan; Sasidharan, Sreenivasan

    2015-01-01

    Candida albicans has become resistant to the commercially available, toxic, and expensive anti-Candida agents that are on the market. These factors force the search for new antifungal agents from natural resources. Cassia spectabilis had been traditionally employed by healers for many generations. The possible mechanisms of the C. spectabilis leaf extract were determined by potassium leakage study and the effect of the extract on the constituents of the cell wall and enzymes as well as the mo...

  9. Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis.

    Torey, Angeline; Vijayarathna, Soundararajan; Jothy, Subramanion L; Gothai, Sivapragasam; Chen, Yeng; Latha, Lachimanan Yoga; Kanwar, Jagat R; Dharmaraj, Saravanan; Sasidharan, Sreenivasan

    2016-01-01

    Candida albicans has become resistant to the commercially available, toxic, and expensive anti-Candida agents that are on the market. These factors force the search for new antifungal agents from natural resources. Cassia spectabilis had been traditionally employed by healers for many generations. The possible mechanisms of the C. spectabilis leaf extract were determined by potassium leakage study and the effect of the extract on the constituents of the cell wall and enzymes as well as the morphological changes on C. albicans cells were studied along with cytotoxicity assays. The cytotoxicity result indicated that the extract is nontoxic as was clearly substantiated by a half maximal inhibitory concentration (IC50) value of 59.10 μg/mL. The treated cells (C. spectabilis extract) demonstrated potassium leakage of 1039 parts per million (ppm) compared to Amphotericin B (AmpB)-treated cells with a released potassium value of 1115 ppm. The effects of the extract on the cell wall proteins illustrated that there were three major types of variations in the expression of treated cell wall proteins: the presence of new proteins, the absence of proteins, and the amount of expressed protein. The activities of two enzymes, α-glucosidase and proteinase, were determined to be significantly high, thereby not fully coinciding with the properties of the antifungal reaction triggered by C. spectabilis. The morphology of C. albicans cells treated with the C. spectabilis extract showed that the cells had abnormalities and were damaged or detached within the microcolonies. Our study verifies C. spectabilis leaf extract as an effective anti-C. albicans agent. PMID:26870686

  10. Efficacy of granulocyte transfusions in the control of systemic candidiasis in the leukopenic host.

    Ruthe, R C; Andersen, B R; Cunningham, B L; Epstein, R B

    1978-09-01

    An experimental canine model was designed to evaluate the effect of granulocyte transfusions on systemic infection with Candida albicans in the granulocytopenic host. Each of a pair of dogs was rendered granulocytopenic with a single intravenous (i.v.) dose of cyclophosphamide (50 mg/kg body weight) and challenged with 10(6) Candida albicans organisms administered i.v. when granulocyte counts were less than or equal to 500/mm3. Granulocytes procured by leukofiltration were infused into six experimental dogs 1, 24, 48, and 72 hr after challenge with Candida. An average of 13 +/- 1.3 X 10(9) granulocytes were administered per infusion, producing an average 1-hr increment of 588 +/- 146 granulocytes/mm3 over the pretransfusion granulocyte count. Experimental and control dogs were killed 96 hr after challenge and organs examined grossly and by quantitative culture techniques to measure the extent of infection. All animals receiving granulocyte transfusions had significantly less tissue infection than nontransfused controls (p less than 0.05). It was concluded that granulocyte transfusions are effective in reducing the severity of infection by Candida albicans during periods of leukopenia. PMID:678666

  11. Resistance mechanisms in fluconazole-resistant Candida albicans isolates from vaginal candidiasis.

    Cernicka, Jana; Subik, Julius

    2006-05-01

    Candida albicans is the most frequently identified yeast species causing mycotic vaginitis. A significant number of vaginal yeast isolates are resistant to azole antifungal agents in vitro. Here we investigated the molecular mechanisms of resistance in 22 randomly selected fluconazole-resistant vaginal C. albicans isolates. Twelve isolates in this collection were found to be cross-resistant to itraconazole and 15 to voriconazole. Most of them also displayed decreased susceptibility to terbinafine. Northern blot analyses revealed overexpression of the MDR1 gene in all isolates, which in some isolates was accompanied by elevated levels of CDR1/CDR2 and ERG11 expression. Sequence analysis of the polymerase chain reaction-amplified ERG11 gene of selected azole-resistant isolates identified D116E and V488I amino acid alterations in Erg11p that are known to be conserved in fluconazole-resistant strains. The results demonstrate that decreased susceptibilities of vaginal yeast isolates to clinically used azole derivatives are the result of a combination of several molecular mechanisms involving drug efflux and alterations in the structure or cellular amount of 14-alpha-lanosterol demethylase. PMID:16621465

  12. Oral candidiasis and hairy leukoplakia correlate with HIV infection in Tanzania

    Schiødt, M; Bakilana, P B; Hiza, J F;

    1990-01-01

    We report a detailed study on oral lesions and their association with the WHO revised provisional case definition of AIDS as well as serologic signs of HIV infection among 186 patients in Dar Es Salaam, Tanzania. The patient material consisted of 39 hospitalized suspected AIDS patients, 44 medica...

  13. Impairment of Host Defense against Disseminated Candidiasis in Mice Overexpressing GATA-3▿

    Haraguchi, Norihiro; Ishii, Yukio; Morishima, Yuko; Yoh, Keigyou; Matsuno, Yosuke; Kikuchi, Norihiro; Sakamoto, Tohru; Takahashi, Satoru; HIZAWA, NOBUYUKI

    2010-01-01

    Candida species are the most common source of nosocomial invasive fungal infections. Previous studies have indicated that T-helper immune response is the critical host factor for susceptibility to Candida infection. The transcription factor GATA-3 is known as the master regulator for T-helper type 2 (Th2) differentiation. We therefore investigated the role of GATA-3 in the host defense against systemic Candida infection using GATA-3-overexpressing transgenic mice. The survival of GATA-3-overe...

  14. Interests of the PET with 18-F.D.G. in infectious pathology: about a case of systemic candidiasis

    We report the interest of the PET with 18F-F.D.G. in the extension evaluation of injuries and in the therapy decision for a patient suffering of a systemic candida. Conclusions: In spite of a lack of recommendations, because of its great sensitivity for the deep infectious centres detection, the PET with 18F-F.D.G. can bring useful information to the management and follow up of the systemic infections. (N.C.)

  15. Candida nivariensis as a New Emergent Agent of Vulvovaginal Candidiasis: Description of Cases and Review of Published Studies.

    Aznar-Marin, Pilar; Galan-Sanchez, Fátima; Marin-Casanova, Pilar; García-Martos, Pedro; Rodríguez-Iglesias, Manuel

    2016-06-01

    Candida nivariensis is a new emergent agent related to human infections in the vaginal tract and other localizations, but the phenotypic characteristics are very similar to Candida glabrata and can be misidentified and underdiagnosed. We described four cases of vulvovaginitis identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and confirmed the results with PCR amplification and sequencing of the entire ITS genomic region (ITS1, ITS2 and 5.8 rRNA). We reinforce the need for new diagnostic tools for the correct identification of yeast infections. PMID:26708316

  16. Sonographic detection of intrarenal and intra-arterial fungus balls in a preterm infant due to systemic candidiasis

    Reither, M.; Schumacher, R.; Hagel, K.J.; Hering, F.

    1983-10-01

    Shortly after birth a preterm infant suffering from aspiration syndrome and subsequent Pseudomonas aeruginosa sepsis showed signs of renal insufficiency and mycotic infection: yeast cells were identified in several urinalyses; there was also an increasing anti-candida IgM antibody titer. At the same time sonographic examinations revealed an increasing echogenicity of the renal cortex and echogenic masses of variable size which did not cause acoustic shadows in both enlarged kidneys. A few days later, we found a right-sided hydronephrosis caused by an intraureteric prevesical mass of equal echogenicity. As we could observe sonographically, the aggressive antimycotic therapy was successful. Eleven weeks later there were signs of cardiac insufficiency. An angiographically demonstrated filling defect, within the pulmonary artery, showed the same sonographic findings as the previously found intrarenal masses. The baby underwent embolectomy and recovered. The thrombotic material contained yeast cells giving evidence of systemic candidasis. Provided appropriate equipment is available, ultrasound today is an excellent non-invasive screening and followup method not only for echoencephalography, but also for more complicated neonatologic problems as seen here. The detailed observation of a changing echogenicity of the renal cortex and pelvis is important and often allows a decisive diagnostic clue before other radiological methods become conclusive.

  17. Correlation between azole susceptibilities, genotypes, and ERG11 mutations in Candida albicans isolates associated with vulvovaginal candidiasis in China.

    Ge, Shu-Hua; Wan, Zhe; Li, Juan; Xu, Jianping; Li, Ruo-Yu; Bai, Feng-Yan

    2010-08-01

    The relationship between susceptibilities to fluconazole and itraconazole and microsatellite CAI genotypes were examined from a total of 154 Candida albicans isolates (97 isolates causing vulvovaginitis in Chinese women and 6 vaginal isolates and 51 oral cavity isolates from asymptomatic carriers). The two dominant genotypes, CAI 30-45 (45 isolates) and CAI 32-46 (33 isolates), associated with vulvovaginitis showed significantly different azole susceptibility patterns with strong statistical support. CAI 32-46 isolates were usually less susceptible to both fluconazole and itraconazole than CAI 30-45 isolates and than the oral isolates with other diversified CAI genotypes. Remarkably different mutation patterns in the azole target gene ERG11 were correspondingly observed among C. albicans isolates representing different genotypes and sources. Isolates with the same or similar CAI genotypes usually possessed identical or phylogenetically closely related ERG11 sequences. Loss of heterozygosity in ERG11 was observed in all the CAI 32-46 isolates but not in the CAI 30-45 isolates and most of the oral isolates sequenced. Compared with the ERG11 sequence of strain SC5314 (X13296), two homozygous missense mutations (G487T and T916C) leading to two amino acid changes (A114S and Y257H) in Erg11p were found in CAI 32-46 isolates. The correlation between azole susceptibility and C. albicans genotype may be of potential therapeutic significance. PMID:20516286

  18. The PAV trial: Does lactobacillus prevent post-antibiotic vulvovaginal candidiasis? Protocol of a randomised controlled trial [ISRCTN24141277

    Hurley Susan

    2004-03-01

    Full Text Available Abstract Background Complementary and alternative medicines are used by many consumers, and increasingly are being incorporated into the general practitioner's armamentarium. Despite widespread usage, the evidence base for most complementary therapies is weak or non-existent. Post-antibiotic vulvovaginitis is a common problem in general practice, for which complementary therapies are often used. A recent study in Melbourne, Australia, found that 40% of women with a past history of vulvovaginitis had used probiotic Lactobacillus species to prevent or treat post-antibiotic vulvovaginitis. There is no evidence that this therapy is effective. This study aims to test whether oral or vaginal lactobacillus is effective in the prevention of post-antibiotic vulvovaginitis. Methods/design A randomised placebo-controlled blinded 2 × 2 factorial design is being used. General practitioners or pharmacists approach non-pregnant women, aged 18–50 years, who present with a non-genital infection requiring a short course of oral antibiotics, to participate in the study. Participants are randomised in a four group factorial design either to oral lactobacillus powder or placebo and either vaginal lactobacillus pessaries or placebo. These interventions are taken while on antibiotics and for four days afterwards or until symptoms of vaginitis develop. Women self collect a vaginal swab for culture of Candida species and complete a survey at baseline and again four days after completing their study medications. The sample size (a total of 496 – 124 in each factorial group is calculated to identify a reduction of half in post-antibiotic vulvovaginitis from 23%, while allowing for a 25% drop-out. An independent Data Monitoring Committee is supervising the trial. Analysis will be intention-to-treat, with two pre-specified main comparisons: (i oral lactobacillus versus placebo and (ii vaginal lactobacillus versus placebo.

  19. Structural characterization of low molecular weight polysaccharide from Astragalus membranaceus and its immunologic enhancement in recombinant protein vaccine against systemic candidiasis.

    Yang, Fan; Xiao, Chunyu; Qu, Jing; Wang, Guiyun

    2016-07-10

    Structure and immunologic enhancement of low molecular weight polysaccharide (LMW-ASP) isolated from the root of Astragalus membranaceus (Fisch) Bge. Were detected in recombinant protein vaccine. Structure analysis of LMW-ASP revealed that LMW-ASP (Mw=5.6kDa) was an acid heteropolysaccharide, which consisted of Glc, Gal, Ara, Xyl and GalA in ratio of 10.0:1.3:1.7:1.0:0.9. Recombinant protein (rP-HSP90C) contained epitope C (LKVIRK) from heat shock protein 90 (HSP90) of Candida albicans was used as a vaccine. The results indicated that LMW-ASP significantly promoted specific antibody titers IgG, IgG1, IgG2b, and IL-2, IL-4, IL-10, IL-12 in sera of mice immunized with rP-HSP90C (p<0.05). It was also found LMW-ASP improved DTH response in HSP90C-injceted mice. More importantly, the mice immunized with rP-HSP90C/LMW-ASP had fewer CFU (colony forming unites) in the kidneys compared to the mice immunized with rP-HSP90C (p<0.05). Therefore, LMW-ASP could be exploited into the novel adjuvant to enhance the efficacy of recombinant protein vaccine. PMID:27106150

  20. Use of Antifungal Saponin SC-2 of Solanum Chrysotrichum for the Treatment of Vulvovaginal Candidiasis: In Vitro Studies and Clinical Experiences

    Herrera-Arellano, Armando; López-Villegas, Edgar O; Rodríguez-Tovar, Aida V; Zamilpa, Alejandro; Jiménez-Ferrer, Enrique; Tortoriello, Jaime; Martínez-Rivera, M Angeles

    2013-01-01

    Saponin SC-2 from Solanum chrysotrichum showed antifungal activity, demonstrated in vitro, which inhibited the growth of dermatophytes, and in vivo, to be effective in the treatment against tinea pedis and pityriasis capitis. Fungistatic and fungicidal activity of saponin SC-2 on Candida albicans and other Candida species, fluconazole and ketoconazole resistaent strains was demostrated. SC-2-associated ultrastructural alterations in several Candida species were observed. An exploratory clinic...

  1. Thrush in newborns

    Candidiasis - oral - newborn; Oral thrush - newborn; Fungal infection - mouth - newborn; Candida - oral - newborn ... chap 56. Lee GE, Kaufman DA, Zaoutis TE. Candidiasis. In: Cherry JD, Harrison GJ, Kaplan SL, Steinbach ...

  2. ПАРОДОНТИТ, АССОЦИИРОВАННОГО С КАНДИДОЗНОЙ ИНФЕКЦИЕЙ : МЕХАНИЗМЫ ФОРМИРОВАНИЯ

    Бейбулатов, Г.; Островская, Л.; Лепилин, А.

    2011-01-01

    Periodontitis associated with candidiasis it is characterized a frequent combination to chronic tonsillitis, pathology of endocrine system, repeated courses of antibacterial therapy or treatment inhalation corticosteroids. Periodontitis associated with candidiasis demands appointment of system antifungal treatment.

  3. Estomatitis subprótesis, prevalencia de candidiasis oral y comparación de su resolución con o sin el empleo de antimicóticos

    Ríos Dueñas, Mabel Rocío

    2014-01-01

    Objetivo: Analizar el tiempo de resolución de la estomatitis protésica con el uso o no de antifúngicos durante el tratamiento, en un grupo de personas que asisten a las clínicas de la Facultad de Odontología de la Universidad Nacional de Colombia. En Colombia, el 93% de las personas con 55 o más años han perdido al menos a uno de sus dientes naturales y la mayoría de ellos utilizan prótesis removible. La estomatitis Protésica (EP) afecta a alrededor del 11-67% de los pacientes ...

  4. Avaliação clínica e laboratorial do gel da Uncaria tomentosa (Unha de Gato) sobre candidose oral Clinical and laboratorial evaluation of Uncaria tomentosa (Cat's Claw) gel on oral candidiasis

    Leonardo Costa de Almeida Paiva; Rodrigo Alves Ribeiro; Jozinete Vieira Pereira; Neuza Maria Cavalcante Oliveira

    2009-01-01

    Na odontologia, a fitoterapia já vem sendo utilizada com sucesso há vários anos. Trata-se de um meio terapêutico que apresenta como vantagem sobre as medicações alopáticas o fato de apresentar reações adversas mínimas. A Uncaria tomentosa é uma planta indígena da floresta Amazônica e de outras áreas tropicais da América do Sul e Central. Tem aplicação no tratamento de diversas patologias, entre elas a candidose. Este trabalho avalia clínico e laboratorialmente a ação do gel da Uncaria tomento...

  5. 乳杆菌活菌制剂用于外阴阴道假丝酵母菌病疗效分析%Analysis of probiotic lactobacillus's effect on vulvovaginal candidiasis

    麦秀云

    2007-01-01

    目的 探讨乳杆菌活菌制剂联合克霉唑治疗外阴阴道假丝酵母菌病的效果.方法 将169例外阴阴道假丝酵母菌病的妇女随机分为2组,分别给予2种治疗方法.A组(85例),乳杆菌活菌胶囊(定君生)与克霉唑联合用药:每日晨1粒定君生胶囊塞入阴道,且每日晚1片克霉唑栓塞入阴道,连续用药10 d;B组(84例),单用克霉唑,每晚1片克霉唑栓塞入阴道,连续用药10 d.2组患者经治疗的5 d、10 d、停药后7 d及月经来潮后复检,临床进行综合评价并行比较.结果 A组治疗第5天的痊愈率和显效率比B组明显增高,差异有显著性(P<0.01);A组治疗第5天的无效率比B组明显降低,差异有显著性(P<0.01).治疗第10天2组间的痊愈率、显效率、有效率差异无显著性.而停药后7 d、月经来潮后的总有效率A组比B组明显增高,复发率A组比B组明显下降,差异有显著性(P<0.05).结论 治疗外阴阴道假丝酵母菌病时,乳杆菌活菌制剂联合克霉唑较单用克霉唑能更快起效,并且有效减少停药后7 d及月经来潮后的复发率.

  6. Preventive effect of probiotic lactobacillus on vulvovaginal candidiasis%乳杆菌活菌制剂预防外阴阴道假丝酵母菌病的疗效观察

    张翠香; 高玉涛; 高华玲

    2005-01-01

    目的:探讨乳杆菌活菌制剂对预防外阴阴道假丝酵母菌病的复发是否有效.方法:将200例单纯性外阴阴道假丝酵母菌病病人随机分为两组,每组100人,观察组用乳杆菌活菌胶囊(定君生)联合硝酸咪康唑栓(达克宁栓)治疗;对照组单一应用硝酸咪康唑栓治疗,对治疗后1个月、3个月的临床效果以及阴道分泌物(10%KOH悬滴法)进行比较,观察其复发率的差别.结果:观察组与对照组的近期治愈率无明显差别,3个月的复发率有明显差别,观察组的复发率明显低于对照组.结论:乳杆菌活菌制剂能够预防外阴阴道假丝酵母菌的复发,治疗中无不良反应.

  7. 性激素对念珠菌性外阴阴道病易感性的影响%Effects of sex hormones on susceptibility to vulvovaginal candidiasis

    牛战琴; 石一复

    2003-01-01

    念珠菌是条件致病性真菌,念珠菌毒力增加,宿主免疫机能失调,局部阴道上皮细胞抗感染能力降低可导致宿主发病,本文从上述三方面综述了性激素对该病易感性的影响.

  8. Brazilian guidelines for the management of candidiasis - a joint meeting report of three medical societies: Sociedade Brasileira de Infectologia, Sociedade Paulista de Infectologia and Sociedade Brasileira de Medicina Tropical

    Arnaldo Lopes Colombo

    2013-06-01

    Full Text Available Candida infections account for 80% of all fungal infections in the hospital environment, including bloodstream, urinary tract and surgical site infections. Bloodstream infections are now a major challenge for tertiary hospitals worldwide due to their high prevalence and mortality rates. The incidence of candidemia in tertiary public hospitals in Brazil is approximately 2.5 cases per 1000 hospital admissions. Due to the importance of this infection, the authors provide a review of the diversity of the genus Candida and its clinical relevance, the therapeutic options and discuss the treatment of major infections caused by Candida. Each topography is discussed with regard to epidemiological, clinical and laboratory diagnostic and therapeutic recommendations based on levels of evidence.

  9. 外阴阴道假丝酵母菌性阴道病阴道局部固有免疫机制研究%Study on inherent immunity in vagina infected with vulvovaginal candidiasis

    彭丹红; 任慕兰

    2009-01-01

    目的 探讨外阴阴道假丝酵母菌性阴道病(VVC)阴道局部固有免疫机制.方法 选取2006年1月至2008年3月健康体检妇女、单纯性VVC患者和复发性VVC(RVVC)患者各20例,采集阴道灌洗液,行HE染色,细胞计数及分类,采用RT-PCR法测定阴道脱落细胞中单核细胞趋化蛋白-1(MCP-1)的mRNA水平.结果 (1)VVC组和RVVC组灌洗液的pH值分别为5.035±0.63和5.430±0.66,与对照组4.572±0.27比较,差异有统计学意义(P<0.05).(2)细胞分类中,VVC组中性粒细胞[(46.45±7.61)%]与对照组和RVVC组[分别为(18.25±3.27)%和(17.7±4.30)%]比较,差异有统计学意义(P<0.01);VVC组单核细胞(5.40±2.30)%,较对照组(3.90±1.68)%和RVVC组(3.70±1.42)%明显上升,差异有统计学意义(P<0.01).(3)对照组、VVC组和RVVC组MCP-1的mRNA表达分别为0.6363±0.0300、0.8338±0.1100、0.5315±0.0600,VVC组MCP-1的mR-NA显著高于对照人群,而RVVC组MCP-1的mRNA显著低于对照人群.结论 (1)阴道pH的改变与阴道的感染相关.(2)假丝酵母菌侵入机体后将导致机体的免疫防御反应,中性粒细胞和单核细胞数量增加并到达炎症部位发挥杀灭病原菌的作用.(3)阴道局部MCP-1低表达相关的免疫防御缺陷可能是RVVC反复发作的机制之一.

  10. Protective Role of Naturally Occurring Interleukin-17A-Producing γδ T Cells in the Lung at the Early Stage of Systemic Candidiasis in Mice ▿ †

    Dejima, Takashi; Shibata, Kensuke; Yamada, Hisakata; Hara, Hiromitsu; Iwakura, Yoichiro; Naito, Seiji; Yoshikai, Yasunobu

    2011-01-01

    Interleukin-17A (IL-17A)-producing γδ T cells differentiate in the fetal thymus and reside in the peripheral tissues, such as the lungs of naïve adult mice. We show here that naturally occurring γδ T cells play a protective role in the lung at a very early stage after systemic infection with Candida albicans. Selective depletion of neutrophils by in vivo administration of anti-Ly6G monoclonal antibody (MAb) impaired fungal clearance more prominently in the lung than in the kidney 24 h after i...

  11. 口服伊曲康唑治疗外阴阴道假丝酵母菌病临床研究进展%Progress in Clinical Research of Itraconazole in the Treatment of Vulvovaginal Candidiasis

    高淑红; 樊尚荣

    2009-01-01

    伊曲康唑是一种广谱的三唑类抗真菌药物,可用于治疗外阴阴道假丝酵母菌病.本文就伊曲康唑口服治疗外阴阴道假丝酵母菌病的作用机制、耐药性、临床疗效及不良反应进行综述.

  12. Interests of the PET with 18-F.D.G. in infectious pathology: about a case of systemic candidiasis; Interets de la TEP au 18-FDG en pathologie infectieuse: a propos d'un cas de candidose systemique

    Avet, J.; Granjon, D.; Prevot, N.; Isnardi, V.; Dubois, F. [Service de medecine nucleaire, CHU de Saint-etienne, (France); Stephan, J.L.; Berger, C. [service de pediatrie, CHU de Saint-etienne, (France)

    2009-05-15

    We report the interest of the PET with {sup 18}F-F.D.G. in the extension evaluation of injuries and in the therapy decision for a patient suffering of a systemic candida. Conclusions: In spite of a lack of recommendations, because of its great sensitivity for the deep infectious centres detection, the PET with {sup 18}F-F.D.G. can bring useful information to the management and follow up of the systemic infections. (N.C.)

  13. 复发性外阴阴道念珠菌病菌种的26S rDNA序列分析%Analysis on recurrent vulvovaginal candidiasis monilia germs of 26S rDNA sequence

    迟绍琴; 许瑞环; 李康; 黄兴国; 陈亦微

    2012-01-01

    目的 探讨基于核糖体基因26S rDNA D1/D2区序列分析法在临床酵母菌菌种鉴定中的应用.方法 收集来源于复发性外阴阴道念珠菌病分泌物标本93株,PCR扩增其26S rDNA D1/D2区,对扩增产物进行序列测定和分析,并与基因库中的基因序列进行同源性比对.结果 所有菌株均鉴定到种,同源性达99%和100%,同属于真菌双核亚界、子囊菌门、酵母菌科的3个属,89株为candida,3株为Kodamaea,1株为Pichia.其中candida中有7个种,38株candida glabrata,23株 candida albicans,16株candida parapsolisis,9株candida metapdilosis,1株candida orthopsilisis,1株 candida tropicalis,1株candida nivariensis;3株Kodamaea ohmeri;1株Pichia kudriavzevii.结论 复发性外阴阴道念珠菌病的病原体主要为candida属的candida glabrata、candida albicans 和candida parapsolisis,非candida albicans占75.27%是其特征;26SrDNA D1/D2区序列分析为临床酵母菌的分子水平鉴定提供了一种准确、可行的方法.%Objective To explore the ribosomes gene based on 26S rDNA D1/D2 area sequence analysis method in the clinical application of yeast strain identification. Methods To collect from recurrent genital vaginal moniiiosis secretion specimens of 93 plants,PCR amplification its 26S rDNA D1/D2 area,the amplification products scries were determined and analyzed,and the genetic sequences with gene pool than homology. Results All strains arc identification to the kind,the homology of 99% and 100%, belong to the fung, dikarya, ascomycota, saccharomycctaccac of this three genera,89 strains for Candida,3 strains for Kodamaca,for Pichia 1 strain. One of seven kinds of Candida,38 strains Candida glabrata, Candida albicans 23 strains, 16 strains Candida parapsolisis, 9 of Candida mctapdilosis, Candida orthopsilisis 1 strain,Candida tropicalis 1 strain, 1 strain Candida nivaricnsis;3 strains Kodamaca ohmcri;Pichia kudriavzevii 1 strain. Conclusion Recurrent genital vaginal moniliosis pathogen of Candida mainly for Candida glabrata, Candida albicans and Candida parapsolisis,the Candida albicans arc take 75. 27% of its characteristic; 26S rDNA D1/D2 area scries analysis for clinical yeast molecular level appraisal provides a accurate and feasible method.

  14. Bilateral chronic fungal dacryocystitis caused by Candida dubliniensis in a neutropenic patient

    Obi, E; Roy, A; Bates, V; Sandy, C

    2006-01-01

    In recent years, candida species other than Candida albicans have emerged as causes of human candidiasis, particularly in HIV‐infected and other immunocompromised people. C dubliniensis, a recently described species closely related to C albicans, first isolated from patients with AIDS in Dublin, has been implicated as an agent of oral candidiasis in HIV‐positive people. However, it has also been recovered from HIV‐negative people, with clinical signs of oral candidiasis and from the genital t...

  15. КОМБИНИРОВАННАЯ ТЕРАПИЯ ОРОФАРИНГЕАЛЬНОГО КАНДИДОЗА И ОЦЕНКА ФАГОЦИТАРНОЙ АКТИВНОСТИ И БИОЦИДНОГО ПОТЕНЦИАЛА НЕЙТРОФИЛОВ ПЕРИФЕРИЧЕСКОЙ КРОВИ

    ВЕРТАКОВА ОЛЬГА ВИКТОРОВНА; АНДАМОВА ОЛЬГА ВЛАДИМИРОВНА; КИСЕЛЕВ АЛЕКСЕЙ БОРИСОВИЧ

    2015-01-01

    The assessment of ability of neutrophils to acquire active forms of oxygen and assessment of phagocytic activity of neutrophils of peripheric blood at patients with oropharyngeal candidiasis against various methods of treatment is carried out. 120 patients with oropharyngeal candidiasis are surveyed and treated. Results which confirm efficiency and expediency of ozono-ultrasonic method in complex treatment of oropharyngeal candidiasis that is confirmed by the obtained clinical laboratorial da...

  16. Comparison of Fluconazole and Clotrimazole in the Treatment of Acute Candida Albicans Vulvovaginitis

    Fatemeh Bahadori; Farzaneh Broomand; Kambiz Diba; Zahra Yekta; Atefe Namaki

    2008-01-01

    Objective: This study compared two antifungal drugs, fluconazole and clotrimazole for the treatment of vulvo vaginal candidiasis. Materials and Methods: This randomized clinical trial was conducted on 120 women with vulvo vaginal candidiasis during a six month period. All patients answered a standard questionnaire containing questions about symptoms of vulvovaginal candidiasis and presence of vaginal discharge and signs of vulvar and vaginal inflammation were documented according to physical ...

  17. Antimicrobial effects of liquid anesthetic isoflurane on Candida albicans

    Armstead Valerie; Parveen Zahida; Logan David A; Lobach Ludmila; Powell Garry; Acheampong Edward; Barodka Viachaslau M; Mukhtar Muhammad

    2006-01-01

    Abstract Candida albicans is a dimorphic fungus that can grow in yeast morphology or hyphal form depending on the surrounding environment. This ubiquitous fungus is present in skin and mucus membranes as a potential pathogen that under opportunistic conditions causes a series of systemic and superficial infections known as candidiasis, moniliasis or simply candidiasis. There has been a steady increase in the prevalence of candidiasis that is expressed in more virulent forms of infection. Alth...

  18. Esophageal strictures during treatment for acute lymphoblastic leukemia.

    Kelly, Kevin

    2012-02-01

    Esophageal stricture is a rare complication of paediatric cancer treatment that usually occurs after esophageal exposure to radiotherapy. We describe 4 cases of esophageal stricture during chemotherapy for acute lymphoblastic leukemia. All patients presented with refractory vomiting and were diagnosed with radiologic contrast studies. None of the patients had received radiotherapy. Esophageal candidiasis was seen in 2 patients but the remaining 2 patients had earlier systemic candidiasis. High-dose dexamethasone may predispose these children to both esophageal candidiasis and peptic esophagitis. The etiology of esophageal strictures during treatment for acute leukemia is likely to be multifactorial but systemic candidiasis may play a significant role.

  19. Vulvovaginal Candida: a study of (a)symptomatic women

    Engberts, M.K.

    2007-01-01

    The research described in this thesis concerns presence of asymptomatic vaginal Candida and vulvovaginal candidiasis. Vulvovaginal candidiasis (VVC) is an infection caused by abnormal growth of yeasts in the mucosa of the female genital tract. Acute vulvar pruritus and vaginal discharge are the usua

  20. Cancer drugs inhibit morphogenesis in the human fungal pathogen, Candida albicans

    Madhushree M Routh

    2013-09-01

    Full Text Available Candida infections are very common in cancer patients and it is a common practice to prescribe antifungal antibiotics along with anticancer drugs. Yeast to hyphal form switching is considered to be important in invasive candidiasis. Targeting morphogenetic switching may be useful against invasive candidiasis. In this study, we report the antimorphogenetic properties of thirty cancer drugs.

  1. Oesophageal candiasis in AIDS

    Yadav, S. P. S.; Ranga, Rupender K.; Singh, Jagat; Yadav, Rohtas

    2003-01-01

    With the explosion in the number of AIDS patients, many of these are likely to consult to otolaryngologists the head & neck is a rather common site to be affected. Sometimes only oesophageal candidiasis is the presenting feature as in the present case which is being reported show typical radiological appearance. the differential diagnosis and treatment of nesophageal candidiasis is briefly discussed

  2. Use of prophylactic antifungals in the immunocompromised host

    Ship, Jonathan A.; Vissink, Arjan; Challacombe, Stephen J.

    2007-01-01

    This literature review assessed the effectiveness of interventions for the prevention of oral candidiasis in immunocompromised patients and in cancer patients treated with radiotherapy and/or chemotherapy. The studies reviewed provide strong evidence that oral candidiasis is associated with greater

  3. 纳米银抗菌凝胶联合氟康唑治疗外阴阴道假丝酵母菌疗效观察%Clinical Observation on the Treatment of Vulvovaginal Candidiasis in Nano Silver Antibacterial Gel Combined with Fluconazole

    张红

    2015-01-01

    目的:探讨纳米银抗菌凝胶联合氟康唑治疗外阴阴道假丝酵母菌病疗效。方法选择门诊确诊的外阴阴道假丝酵母菌患者58例随机分为治疗组和对照组,治疗组氟康唑单次口服150mg,纳米银抗菌凝胶连用6d。对照组纳米银抗菌凝胶连用6d,对比疗效。结果两组停药后7d总有效率为93.3%和82.1%,两组在下次月经干净后总有效率为93.3%和78.6%。结论纳米银抗菌凝胶联合氟康唑治疗外阴阴道假丝酵母菌具有较好的疗效。%Objective To explore the nano silver antibacterial gel with fluconazole therapy vulva vagina candida disease curative ef ect. Methods Clinic diagnosis of patients with vulva vagina candida 58 cases were randomly divided into treatment group and control group, treatment group fluconazole single oral 150 mg, a laparoscope, usual y connected to a nano silver antibacterial gel 6 days. A laparoscope, usual y connected to a control group of nano silver antibacterial gel 6 days, compare the curative ef ect. Results Two groups of seven days after the drug was stopped the total ef ective rate was 93.3%and 82.1%, the two groups in next time menstruation clean after the total ef ective rate was 93.3%and 93.3%. Conclusion Nano silver antibacterial gel with fluconazole therapy vulva vagina candida has good curative ef ect.

  4. Observation on Long-Term Follow up Result of Treatment of Recurrent Vulvovaginal Candidiasis with Ozone%臭氧治疗复发性外阴阴道假丝酵母菌病的远期疗效观察

    黄奕璇

    2013-01-01

    Objective To observe the long-term follow up result of treatment of recurrent vulvovaginal candidasis( RVVC) with ozone,and to cure RWC actually. Methods 152 cases of patients with RVVC in our hospital in recent years were divided into two groups,76 cases in the control group,and 76 cases in the treatment group. The patients in the control group were treated by Clotrimazole and Fluconazole,yet patients in the treatment group were treated by ozone and Fluconazole. Then the treatment efficiency and relapse rate of the two groups were compared in preliminary treatment and maintenance treatment. Results In preliminary treatment,the total effective rate was 100% in the treatment group,with 98.7% in the control group,the difference was not significant (P >0.05). In maintenance treatment,the total effective rate was 100% in the treatment group,with 96.1% in the control group,the difference was not significant (P >0. 05). But half a year after the treatment, the relapse rate was 1.3% in treatment group, with 14.5% in control group,the difference was statistically significant in long-term follow up result of treatment (P <0.05). Conclusion This research proved that the treatment of RVVC with ozone was effective. It cured RWC actually, showing advantages of simple and safe operation,no side effects. The ozone therapy had high clinical value.%目的 观察臭氧治疗复发性外阴阴道假丝酵母菌病(RVVC)的远期疗效,从真正意义上治愈RVVC.方法 将揭阳市人民医院门诊收治的RVVC患者152例随机分为对照组76例,治疗组76例,对照组采用传统治疗,予克霉唑栓剂配伍氟康唑口服,治疗组予臭氧治疗配伍氟康唑口服,两组均分初步治疗和巩固治疗,从疗效及复发率比较两组患者的治疗效果.结果 初步治疗:总有效率治疗组100%,对照组98.7%,P>0.05,差异无统计学意义;巩固治疗:总有效率治疗组100%,对照组96.1%,P>0.05,差异无统计学意义,但在全部治疗结束后半年观察远期疗效,比较两组的复发率,治疗组1.3%,对照组14.5%,P<0.05,差异有统计学意义.结论 本研究证明臭氧治疗RVVC效果肯定,从真正意义上治愈了RVVC,且操作简单、安全、无副作用,具有很高的临床价值.

  5. Efficacy of Ciclopirox Olamine Vaginal Suppositories Combined with Fluconazole in the Treatment of Vul-vovaginal Candidiasis%环吡酮胺阴道栓联合氟康唑治疗外阴阴道假丝酵母菌的疗效观察

    王琇

    2015-01-01

    目的:观察环吡酮胺阴道栓联合氟康唑治疗外阴阴道假丝酵母菌的疗效观察。方法:74例外阴阴道假丝酵母菌( VVC)感染患者随机分为两组各37例。对照组给予口服氟康唑联合阴道内放置盐酸特比萘芬阴道泡腾片,观察组给予口服氟康唑联合环吡酮胺阴道栓治疗,观察对比两组近期(7 d)、远期(1、3个月)的临床疗效及用药安全性。结果:治疗7 d后,两组临床总有效率差异无统计学意义( P>0.05);1个月后随访,两组复发率比较,差异无统计学意义( P>0.05),3个月后随访,观察组复发率为2.7%,明显低于对照组的22.2%(P0. 05);after the 1-month follow-up,the recurrence rate of the two groups had no significant difference(P>0. 05);after the 3-month follow-up,the re-currence rate of the observation group(2. 7%)was significantly lower than that of the control group(22. 2%,P<0. 05). The two groups showed no serious adverse drug reactions. Conclusion:Fluconazole combined with ciclopirox olamine vaginal suppositories in the treatment of vulvovaginal candidasis exhibits promising curative effect and low recurrence rate without serious adverse reactions.

  6. Theoretical Study of Applying Strengthening Healthy Energy and Eliminating Dampness Method in the Treatment of Recurrent Vulvovaginal Candidiasis%扶正祛湿法治疗复发性外阴阴道念珠菌病的理论研究

    卫晓玉

    2016-01-01

    目前西医治疗复发性外阴阴道念珠菌病,困境与前景并存.本文分析总结中医对本病认识的病因病机与治则,并结合西医对该病的认识与已有研究,探讨以扶正祛湿法为主的中医疗法作用机理,以期对治疗思路提供新的启示.

  7. IgA, IgE e subclasses de IgG anti-Candida albicans no soro e lavado vaginal de pacientes com candidíase vulvovaginal IgA, IgE and IgG subclasses to Candida albicans in serum and vaginal fluid from patients with vulvovaginal candidiasis

    Ricardo José Victal de Carvalho; Cristiane Martins Cunha; Deise Aparecida de Oliveira Silva; Mônica Camargo Sopelete; Jane Eire Urzedo; Tomaz Aquino Moreira; Paula de Souza Abreu Moraes; Ernesto Akio Taketomi

    2003-01-01

    OBJETIVO: Determinar níveis de anticorpos IgA, IgE, IgG e subclasses (IgG1, IgG4) específicos a C. albicans no soro e lavado vaginal de mulheres com ou sem candidíase vulvovaginal para avaliar o papel destes anticorpos na imunopatogênese desta doença. MÉTODOS: Foram selecionadas 30 mulheres com sintomas clínicos de candidíase vulvovaginal (15 com cultura de secreção vaginal positiva para C. albicans, 11 com cultura negativa e quatro com cultura positiva para Candida não-albicans) e 12 mulhere...

  8. Genetics Home Reference: autoimmune polyglandular syndrome, type 1

    ... by mistake. In most cases, the signs and symptoms of autoimmune polyglandular syndrome, type 1 begin in childhood or adolescence. This condition is characterized by three specific features: mucocutaneous candidiasis, hypoparathyroidism, and Addison disease. Affected individuals typically have ...

  9. Cytolytic Vaginosis: Misdiagnosed as Candidal Vaginitis

    M. Sinan Beksac

    2004-01-01

    Full Text Available Objectives: In this study, 210 women with vaginal discharge and other symptoms/signs of genital pathology suggestive of vulvovaginal candidiasis (VVC were involved in order to distinguish true VVC and cytolytic vaginosis (CV cases.

  10. Angular Cheilitis

    ... A This image displays a frequent location for candida infection (angular cheilitis), the corners of the mouth. Overview ... infection, those affected may also have thrush (oral candidiasis). The areas are generally slightly painful. The condition ...

  11. Vaginal Yeast Infections (For Parents)

    ... infection caused by a type of fungus called candida albicans . Yeast infections usually happen in warm, moist parts of the ... fungus can grow. Doctors call this candida overgrowth candidiasis (pronounced: can-dih-DYE-uh-sis) Candida can ...

  12. Oral Thrush (For Parents)

    ... About Oral Thrush Symptoms Prevention Treatment en español Candidiasis bucal About Oral Thrush Oral thrush is a ... digestive tract can overgrow and lead to an infection. Candida overgrowth also causes diaper rash and vaginal (yeast) ...

  13. Medications that Weaken Your Immune System and Fungal Infections

    ... 7 , 8 Pneumocystis pneumonia (PCP) 9 , 10 Invasive Candida infection 7 Invasive aspergillosis 7 Other fungal infections, such as oral candidiasis (thrush) , are usually not life threatening. 11 What ...

  14. Comparison Between Virulence Factors of Candida albicans and Non-Albicans Species of Candida Isolated from Genitourinary Tract

    Udayalaxmi,; Jacob, Shani; D’Souza, Diney

    2014-01-01

    Background: Candida spp. is frequently isolated from cases of vulvovaginal candidiasis and catheter associated UTI. C.albicans is the most frequently isolated species but non-albicans species of candida are gaining clinical significance.

  15. Vaginal Yeast Infection

    ... Centers for Diseases Control and Prevention. Micrograph showing Candida albicans from a patient with vaginal candidiasis, also known ... caused by an overgrowth of a fungus called Candida albicans in the vagina. Candida is yeast, which is ...

  16. Vaginal yeast infection

    Yeast infection - vagina; Vaginal candidiasis; Monilial vaginitis ... Most women have a vaginal yeast infection at some time. Candida albicans is a common type of fungus. It is often found in small amounts in the ...

  17. Phage displaying epitope of Candida albicans HSP90 and serodiagnosis

    杨琼; 王丽; 卢大宁; 邢沈阳; 尹东; 朱筱娟

    2004-01-01

    @@ Recently, the frequent use of immunosuppressants and chemotherapeutic drugs for cancers has caused an increase in the frequency of life-threatening systemic candidiasis.1 Studies by Matthews et al2 indicated HSP90 fragments are major targets for the immune system in infection due to C. albicans, and anti-epitope LKVIRK of HSP90 antibody is a serological marker for diagnosis of invasive candidiasis. Cloning and sequencing HSP90 antigen revealed that the linear epitope LKVIRK, localized near the C-terminus of the 47 kDa protein which circulates in the sera of patients with invasive candidiasis, as a heat-stable breakdown product of large more heat-labile antigen HSP90.2 In this study, epitope LKVIRK was displayed on the surface of phage fd to develop a new serological test for systemic candidiasis.

  18. The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection

    Brittany E Goldberg; Mongodin, Emmanuel F.; Cheron E Jones; Chung, Michelle; Claire M Fraser; Tate, Anupama; Steven L Zeichner

    2015-01-01

    The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed population, the spectrum of potential oral disease is even broader, encompassing candidiasis, necrotizing gingivitis, parotid gland enlargement, Kaposi’s sarcoma, oral warts and other diseases. He...

  19. Sap6, a secreted aspartyl proteinase, participates in maintenance the cell surface integrity of Candida albicans

    Buu, Leh-Miauh; Chen, Yee-Chun

    2013-01-01

    Background The polymorphic species Candida albicans is the major cause of candidiasis in humans. The secreted aspartyl proteinases (Saps) of C. albicans, encoded by a family of 10 SAP genes, have been investigated as the virulent factors during candidiasis. However, the biological functions of most Sap proteins are still uncertain. In this study, we applied co-culture system of C. albicans and THP-1 human monocytes to explore the pathogenic roles and biological functions of Sap proteinases. R...

  20. Soft X-ray tomography of phenotypic switching and the cellular response to antifungal peptoids in Candida albicans

    Uchida, Maho; McDermott, Gerry; Wetzler, Modi; Le Gros, Mark A.; Myllys, Markko; Knoechel, Christian; Barron, Annelise E.; Larabell, Carolyn A.

    2009-01-01

    The opportunistic pathogen Candida albicans can undergo phenotypic switching between a benign, unicellular phenotype and an invasive, multicellular form that causes candidiasis. Increasingly, strains of Candida are becoming resistant to antifungal drugs, making the treatment of candidiasis difficult, especially in immunocompromised or critically ill patients. Consequently, there is a pressing need to develop new drugs that circumvent fungal drug-resistance mechanisms. In this work we used sof...

  1. Comparison of the extracellular polymeric substances of Candida albicans and Candida dubliniensis biofilms

    Henriques, Mariana; M.C. Fernandes; Azeredo, Joana; Oliveira, Rosário

    2006-01-01

    Candida albicans and Candida dubliniensis live as benign commensal organisms in the oral cavity of both healthy and unhealthy individuals behaving, under certain conditions, as opportunistic pathogens, causing candidiasis. These two Candida species have been mismatched for years, but recently Candida dubliniensis was recovered from the mouth of imunnosupressed patients and identified as a different species. Candidiasis is usually related with the Candida capacity of forming biofilms on inert ...

  2. Urinary tract infections and Candida albicans

    BEHZADI, Payam; BEHZADI, Elham; Ranjbar, Reza

    2015-01-01

    Introduction Urinary tract candidiasis is known as the most frequent nosocomial fungal infection worldwide. Candida albicans is the most common cause of nosocomial fungal urinary tract infections; however, a rapid change in the distribution of Candida species is undergoing. Simultaneously, the increase of urinary tract candidiasis has led to the appearance of antifungal resistant Candida species. In this review, we have an in depth look into Candida albicans uropathogenesis and distribution o...

  3. Single and multiple dose Fluconazole in the treatment of candidia vulvovaginitis: a prospective comparative study

    Ashrafinia M; Behdani R; Komijani Z

    2007-01-01

     Background: Vulvovaginal candidiasis, the most common type of vaginitis, is usually caused by Candidia albicans. Patients experience a variety of symptoms. There are many types of vulvovaginal candidiasis with various microbial causes, symptoms, host circumstances, recurrence rates, and responses to treatment. The purpose of this study was to find the best method of treatment of complicated vaginitis as determined by its high prevalence, varying symptoms and signs and patient compla...

  4. Fungal inhibitory effect of Citrus Limon peel essential oil on Candida albicans

    Iwan Hernawan; Desiana Radithia; Priyo Hadi; Diah Savitri Ernawati

    2015-01-01

    Background: Oral candidiasis is an opportunistic infections due to Candida albicans that often found in people with HIV/AIDS. Anti-fungi, polyne and azole, are used in the treatment of oral candidiasis, but often cause persistence and recurrence. Citrus Limon peel contains terpenoids capable of inhibiting the synthesis of ergosterol, a component of the fungal cell wall that helps to maintain cell membrane permeability. Essential oil derived from citrus limon peel, thus, is expected to inhibit...

  5. Candida Infections of the Genitourinary Tract

    Achkar, Jacqueline M.; Fries, Bettina C.

    2010-01-01

    Summary: All humans are colonized with Candida species, mostly Candida albicans, yet some develop diseases due to Candida, among which genitourinary manifestations are extremely common. The forms of genitourinary candidiasis are distinct from each other and affect different populations. While vulvovaginal candidiasis affects mostly healthy women, candiduria occurs typically in elderly, hospitalized, or immunocompromised patients and in neonates. Despite its high incidence and clinical relevan...

  6. Cerebral candiasis: CT studies in a case of brain abscess and granuloma due to candida albicans

    The CT features of a young female patient suffering from systemic candidiasis with intracerebral manifestation are reported. The definite diagnosis was made by spinal fluid cultures. The diffuse granulomatous lesions as well as an abscess formation remitted after specific therapy with 5-fluorocytosine and amphotericin B for now more than 1 year. In contrast to reports of other cases with mycosis of the central nervous system this case of candidiasis shows lesions of primarily increased attenuation coefficients. (orig.)

  7. Cerebral candiasis: CT studies in a case of brain abscess and granuloma due to candida albicans

    Thron, A.; Wiethoelter, H.

    1982-08-01

    The CT features of a young female patient suffering from systemic candidiasis with intracerebral manifestation are reported. The definite diagnosis was made by spinal fluid cultures. The diffuse granulomatous lesions as well as an abscess formation remitted after specific therapy with 5-fluorocytosine and amphotericin B for now more than 1 year. In contrast to reports of other cases with mycosis of the central nervous system this case of candidiasis shows lesions of primarily increased attenuation coefficients.

  8. Echinocandins: A ray of hope in antifungal drug therapy

    Grover Neeta

    2010-01-01

    Full Text Available Invasive fungal infections are on the rise. Amphotericin B and azole antifungals have been the mainstay of antifungal therapy so far. The high incidence of infusion related toxicity and nephrotoxicity with amphotericin B and the emergence of fluconazole resistant strains of Candida glabrata egged on the search for alternatives. Echinocandins are a new class of antifungal drugs that act by inhibition of β (1, 3-D- glucan synthase, a key enzyme necessary for integrity of the fungal cell wall. Caspofungin was the first drug in this class to be approved. It is indicated for esophageal candidiasis, candidemia, invasive candidiasis, empirical therapy in febrile neutropenia and invasive aspergillosis. Response rates are comparable to those of amphotericin B and fluconazole. Micafungin is presently approved for esophageal candidiasis, for prophylaxis of candida infections in patients undergoing hematopoietic stem cell transplant (HSCT and in disseminated candidiasis and candidemia. The currently approved indications for anidulafungin are esophageal candidiasis, candidemia and invasive candidiasis. The incidence of infusion related adverse effects and nephrotoxicity is much lower than with amphotericin B. The main adverse effect is hepatotoxicity and derangement of serum transaminases. Liver function may need to be monitored. They are, however, safer in renal impairment. Even though a better pharmacoeconomical choice than amphotericin B, the higher cost of these drugs in comparison to azole antifungals is likely to limit their use to azole resistant cases of candidial infections and as salvage therapy in invasive aspergillosis rather than as first line drugs.

  9. Anidulafungin in the treatment of invasive fungal infections

    Kathryn Sabol

    2008-03-01

    Full Text Available Kathryn Sabol, Tawanda GumboUniversity of Texas Southwestern Medical Center, Dallas, TX, USAAbstract: More antifungal agents have reached clinical use in the past two decades than at any other time. The echinocandins have been a welcome addition to this group, with the latest being anidulafungin. There are several lines of evidence to support anidulafungin’s role as primary therapy for the treatment of invasive candidiasis in non-neutropenic patients, and as alternative therapy to fluconazole in patients with esophageal candidiasis with azole intolerance or triazole-resistant Candida. Pharmacokinetic–pharmacodynamic studies in animals have demonstrated superior efficacy, defined as maximal microbial kill, when compared to fluconazole, regardless of the fluconazole susceptibility of the Candida species. These studies, as well as dose-effect studies in patients, also support the currently recommended dose of anidulafungin. A well designed randomized controlled trial has demonstrated anidulafungin’s efficacy in patients with invasive candidiasis. In this paper, we argue that anidulafungin may be preferable to fluconazole for the treatment of candidemia. However, as of yet, the difference between anidulafungin and the other two licensed echinocandins as first-line therapy for invasive candidiasis is unclear. On the other hand, there is insufficient evidence as of yet to support first-line use of anidulafungin in patients with neutropenia or aspergillosis.Keywords: anidulafungin, pharmacokinetics-pharmacodynamics, efficacy, candidiasis

  10. Candidal Infection of the Gingiva Mimicking Desquamative Gingivitis: A Case Report

    Potluri, Sushma; Surapaneni, Hemchand; Basha, Md.Hafeez; Davanapelly, Pavithra

    2016-01-01

    There has been an increase in the occurrence of fungal infections in humans in the recent years due to the discrete use of broad spectrum antibiotics and immunosuppressive therapies. The genus candida is the most frequently found fungi in humans. Candida albicans is a mucosal microbiota although it can cause infections which can be mucosal or life threatening infections in susceptible individuals. Candidiasis is the most common oral opportunistic fungal infection in humans. Candidiasis usually affects oral mucosa (buccal mucosa) and hard palate. Candidiasis affecting gingiva is not so common, but when it occurs, it is often misdiagnosed as desquamative gingivitis because of its clinical appearance. This paper discusses a case of Candidal infection of gingiva that mimics desquamative gingivitis. PMID:27135011

  11. Isolation of Candida albicans and their sensitivity to antifungal agents.

    Tsai, S S; Liu, C I; Chang, C F; Tsai, Y H

    1982-02-01

    During 1978 and 1979 outbreaks of candidiasis were encountered in about 500,000 broiler chickens on 30 farms, 30,000 guinea fowl on 4 farms, 500 geese on 1 farm, and 10 penguins in a zoo. This represents the first report of avian candidiasis in Taiwan. Diagnoses were based on typical gross and microscopic pathology consistent isolation of Candida albicans from crop and esophageal lesions, virulence of isolates in rabbits, identification of the isolates as C. albicans serotype A by chlamydospore and germ tube formation, fermentation tests, and agglutination by specific antisera, and the lack of any sign of fowl pox or trichomoniasis. The upsurge of candidiasis is attributed to the modernization stressors which interact deleteriously with opportunistic pathogens. In vitro sensitivity testing recorded the following order of effectiveness against 20 isolates: nystatin greater than gentian violet greater than copper sulfate = propionic acid = p-hydroxy benzoic acid greater than calcium propionate = propylene glycol. PMID:7042231

  12. Candidal Infection of the Gingiva Mimicking Desquamative Gingivitis: A Case Report.

    Yalamanchili, Pallavi Samatha; Potluri, Sushma; Surapaneni, Hemchand; Basha, Md Hafeez; Davanapelly, Pavithra

    2016-03-01

    There has been an increase in the occurrence of fungal infections in humans in the recent years due to the discrete use of broad spectrum antibiotics and immunosuppressive therapies. The genus candida is the most frequently found fungi in humans. Candida albicans is a mucosal microbiota although it can cause infections which can be mucosal or life threatening infections in susceptible individuals. Candidiasis is the most common oral opportunistic fungal infection in humans. Candidiasis usually affects oral mucosa (buccal mucosa) and hard palate. Candidiasis affecting gingiva is not so common, but when it occurs, it is often misdiagnosed as desquamative gingivitis because of its clinical appearance. This paper discusses a case of Candidal infection of gingiva that mimics desquamative gingivitis. PMID:27135011

  13. The burden of serious fungal diseases in Russia.

    Klimko, N; Kozlova, Y; Khostelidi, S; Shadrivova, O; Borzova, Y; Burygina, E; Vasilieva, N; Denning, D W

    2015-10-01

    The incidence and prevalence of fungal infections in Russia is unknown. We estimated the burden of fungal infections in Russia according to the methodology of the LIFE program (www.LIFE-worldwide.org). The total number of patients with serious and chronic mycoses in Russia in 2011 was three million. Most of these patients (2,607,494) had superficial fungal infections (recurrent vulvovaginal candidiasis, oral and oesophageal candidiasis with HIV infection and tinea capitis). Invasive and chronic fungal infections (invasive candidiasis, invasive and chronic aspergillosis, cryptococcal meningitis, mucormycosis and Pneumocystis pneumonia) affected 69,331 patients. The total number of adults with allergic bronchopulmonary aspergillosis and severe asthma with fungal sensitisation was 406,082. PMID:26449508

  14. Oral mucosal cell response to Candida albicans in transgenic mice expressing HIV-1.

    de Repentigny, Louis; Lewandowski, Daniel; Aumont, Francine; Hanna, Zaher; Jolicoeur, Paul

    2009-01-01

    Controlled studies on the immunopathogenesis of mucosal candidiasis in HIV infection have been hampered by the lack of a relevant animal model. We have previously reported that oral Candida infection in CD4C/HIV transgenic mice expressing gene products of HIV-1 in immune cells and developing an AIDS-like disease closely mimics oropharyngeal candidiasis in human HIV infection. The role of defective dendritic cells and CD4+ T cells in impaired induction of protective immunity and in the phenotype of chronic oral carriage of C. albicans can now be investigated under controlled conditions in these transgenic mice. PMID:19089395

  15. Studies of Immune Responses in Candida vaginitis.

    De Bernardis, Flavia; Arancia, Silvia; Sandini, Silvia; Graziani, Sofia; Norelli, Sandro

    2015-01-01

    The widespread occurrence of vaginal candidiasis and the development of resistance against anti-fungal agents has stimulated interest in understanding the pathogenesis of this disease. The aim of our work was to characterize, in an animal model of vaginal candidiasis, the mechanisms that play a role in the induction of mucosal immunity against C. albicans and the interaction between innate and adaptive immunity. Our studies evidenced the elicitation of cell-mediated immunity (CMIs) and antibody (Abs)-mediated immunity with a Th1 protective immunity. An immune response of this magnitude in the vagina was very encouraging to identify the proper targets for new strategies for vaccination or immunotherapy of vaginal candidiasis. Overall, our data provide clear evidence that it is possible to prevent C. albicans vaginal infection by active intravaginal immunization with aspartyl proteinase expressed as recombinant protein. This opens the way to a modality for anti-Candida protection at the mucosa. The recombinant protein Sap2 was assembled with virosomes, and a vaccine PEVION7 (PEV7) was obtained. The results have given evidence that the vaccine, constituted of virosomes and Secretory aspartyl proteinase 2 (Sap2) (PEV7), has an encouraging therapeutic potential for the treatment of recurrent vulvovaginal candidiasis. PMID:26473934

  16. Antimicrobial effects of liquid anesthetic isoflurane on Candida albicans

    Armstead Valerie

    2006-11-01

    Full Text Available Abstract Candida albicans is a dimorphic fungus that can grow in yeast morphology or hyphal form depending on the surrounding environment. This ubiquitous fungus is present in skin and mucus membranes as a potential pathogen that under opportunistic conditions causes a series of systemic and superficial infections known as candidiasis, moniliasis or simply candidiasis. There has been a steady increase in the prevalence of candidiasis that is expressed in more virulent forms of infection. Although candidiasis is commonly manifested as mucocutaneous disease, life-threatening systemic invasion by this fungus can occur in every part of the body. The severity of candidal infections is associated with its morphological shift such that the hyphal morphology of the fungus is most invasive. Of importance, aberrant multiplication of Candida yeast is also associated with the pathogenesis of certain mucosal diseases. In this study, we assessed the anti-candidal activity of the volatile anesthetic isoflurane in liquid form in comparison with the anti-fungal agent amphotericin B in an in vitro culture system. Exposure of C. albicans to isoflurane (0.3% volume/volume and above inhibited multiplication of yeast as well as formation of hyphae. These data suggest development of potential topical application of isoflurane for controlling a series of cutaneous and genital infections associated with this fungus. Elucidiation of the mechanism by which isoflurane effects fungal growth could offer therapeutic potential for certain systemic fungal infections.

  17. Studies of Immune Responses in Candida vaginitis

    Flavia De Bernardis

    2015-10-01

    Full Text Available The widespread occurrence of vaginal candidiasis and the development of resistance against anti-fungal agents has stimulated interest in understanding the pathogenesis of this disease. The aim of our work was to characterize, in an animal model of vaginal candidiasis, the mechanisms that play a role in the induction of mucosal immunity against C. albicans and the interaction between innate and adaptive immunity. Our studies evidenced the elicitation of cell-mediated immunity (CMIs and antibody (Abs-mediated immunity with a Th1 protective immunity. An immune response of this magnitude in the vagina was very encouraging to identify the proper targets for new strategies for vaccination or immunotherapy of vaginal candidiasis. Overall, our data provide clear evidence that it is possible to prevent C. albicans vaginal infection by active intravaginal immunization with aspartyl proteinase expressed as recombinant protein. This opens the way to a modality for anti-Candida protection at the mucosa. The recombinant protein Sap2 was assembled with virosomes, and a vaccine PEVION7 (PEV7 was obtained. The results have given evidence that the vaccine, constituted of virosomes and Secretory aspartyl proteinase 2 (Sap2 (PEV7, has an encouraging therapeutic potential for the treatment of recurrent vulvovaginal candidiasis.

  18. β-Microseminoprotein endows post coital seminal plasma with potent candidacidal activity by a calcium- and pH-dependent mechanism

    Edström Hägerwall, Anneli; Rydengård, Victoria; Fernlund, Per;

    2012-01-01

    The innate immune factors controlling Candida albicans are mostly unknown. Vulvovaginal candidiasis is common in women and affects approximately 70-75% of all women at least once. Despite the propensity of Candida to colonize the vagina, transmission of Candida albicans following sexual intercourse...

  19. Antifungal prophylaxis during neutropenia and immunodeficiency.

    Lortholary, O; Dupont, B

    1997-01-01

    Fungal infections represent a major source of morbidity and mortality in patients with almost all types of immunodeficiencies. These infections may be nosocomial (aspergillosis) or community acquired (cryptococcosis), or both (candidiasis). Endemic mycoses such as histoplasmosis, coccidioidomycosis, and penicilliosis may infect many immunocompromised hosts in some geographic areas and thereby create major public health problems. With the wide availability of oral azoles, antifungal prophylact...

  20. Dermatomycoses In Diabetics - A Clinical Study

    Parthiban K; Janaki C; Selvi Sentamil G; Janaki V R

    1998-01-01

    Two hundred diabetics including 153 non- insulin dependent diabetes mellitus and 47 insulin dependent diabetes mellitus patients, were screened for mycoses. Candidiasis was the commonest mycoses, followed by dermatophtoses and pityriasis versicolor. The fasting blood glucose level was observed to be higher in candidialsis compared to other mycoses.

  1. Enhanced activity of antifungal drugs using natural phenolics against yeast strains of Candida and Cryptococcus

    Candidiasis and cryptococcosis are diseases of widening global incidence as a result of increasing immunosuppressive disorders, such as AIDS. An enduring problem for treatment of these mycoses is recurrent development of resistance to introduced antifungal drugs. We examined the potential for enhan...

  2. The role of lactobacillus probiotics in the treatment or prevention of urogenital infections--a systematic review.

    Abad, C L; Safdar, N

    2009-06-01

    Probiotics are increasingly being used to treat and prevent urogenital infections. However, a critical assessment of their efficacy in major urogenital infections is lacking. We report the results of a systematic review to determine the efficacy of probiotics for prevention or treatment of three major urogenital infections: bacterial vaginosis, vulvovaginal candidiasis, and urinary tract infection. Using multiple computerized databases, we extracted data from clinical trials using a lactobacillus-containing preparation to either prevent or treat a urogenital infection. Of 25 included studies, 18 studies used lactobacillus preparations for treatment or prevention of urogenital infections and seven studies focused solely on vaginal colonization. Four studies included patients with vaginal candidiasis, five included patients with urinary tract infections, and eight included patients with bacterial vaginosis. One included several types of genitourinary infections. Overall, lactobacilli were beneficial for the treatment of patients with bacterial vaginosis. No clear benefit was seen for candidiasis or urinary tract infection. Studies were heterogeneous, with some limited by a small population size. In conclusion, the use of certain lactobacillus strains such as L. rhamnosus GR-1 and L. reuteri for prevention and treatment of recurrent urogenital infection is promising, especially for recurrent bacterial vaginosis. Scant data on the use of probiotics for urinary tract infection and vulvovaginal candidiasis precludes definitive recommendations. Further research and larger studies on types of lactobacilli strains, dosage of lactobacilli, optimal route and vehicle of administration are needed. PMID:19567343

  3. Tinea faciei caused by Trichophyton mentagrophytes in a 20-day-old neonate.

    Malhotra, Sita; Malhotra, Suresh Kumar; Aggarwal, Yukti

    2015-12-01

    Although candidiasis in newborns is not uncommon, superficial dermatophyte infections of infants is quite rare. The causative agents of neonatal tinea reported in various case studies have been Trichophyton rubrum, Microsporum canis, Microsporum gypseum, and Trichophyton violaceum. To the best of our knowledge, no case report of neonatal tinea faciei caused by Trichophyton mentagrophytes has been reported earlier. PMID:26904450

  4. Tinea faciei caused by Trichophyton mentagrophytes in a 20-day-old neonate

    Sita Malhotra; Suresh Kumar Malhotra; Yukti Aggarwal

    2015-01-01

    Although candidiasis in newborns is not uncommon, superficial dermatophyte infections of infants is quite rare. The causative agents of neonatal tinea reported in various case studies have been Trichophyton rubrum, Microsporum canis, Microsporum gypseum, and Trichophyton violaceum. To the best of our knowledge, no case report of neonatal tinea faciei caused by Trichophyton mentagrophytes has been reported earlier.

  5. ETUDE PHENOTYPIQUE E T MOLECULAIRE DES CA NDIDOSES VULVO - VAGINALES CHEZ DES P ATIENTES AU MAROC.

    H. BOURA

    2013-02-01

    Full Text Available In vulvovaginal pathology, vaginal candidiasis is the most common etiologies. Candida albicans is involved in more than 75% in this pathology. Our study included 151 vaginal swabs from women atMedical center of the Institute Pasteur in Morocco. Each vaginal swab was exanimated by direct test and culture on specific media.Out 151 samples 18 were positive (11.9% by direct examination. However, culture results showed that 29/151(19.2%positive cases. The identification of positivecultures showed that Candida albicans is present in 21 cases 72%(21/29 of vaginal candidiasis followed byCandida glabrata with a frequency of 24% (7/29, andCandida tropicalis with a frequency of 3.4 % (1/29 .The clinical symptoms were essentially theleucorrhea, itching and sensation of burning.The most age range affected by vaginalcandidiasis is between 22 and 37 years.Our result showed that the prevalence of vaginal candidiasis was 19% in Moroccan patient with a predominance ofCandida albicans and a sharp increase invaginal candidiasis due to non albicans species.These results will have an impact on thediagnosis and treatement of vaginal candidisis in Morocco.

  6. Miconazole-loaded solid lipid nanoparticles: formulation and evaluation of a novel formula with high bioavailability and antifungal activity

    Aljaeid, Bader Mubarak; Hosny, Khaled Mohamed

    2016-01-01

    Background and objective Miconazole is a broad-spectrum antifungal drug that has poor aqueous solubility (2.5-fold. Conclusion MN-SLN was more efficient in the treatment of candidiasis with enhanced oral bioavailability and could be a promising carrier for the oral delivery of miconazole. PMID:26869787

  7. Chronic invasive aspergillosis of paranasal sinuses: A case report with review of literature

    Avinash P Tamgadge

    2012-01-01

    Full Text Available Aspergillosis of the nasal and paranasal sinuses is recognized as being second to candidiasis, among opportunistic fungal infections in immunocompromised patients. However, invasive variant in normal and mildly immunocompromised hosts is a very rare occurrence. We report one such case of aspergillosis involving paranasal sinuses in mildly immunocompromised patient.

  8. DESIGN AND EVALUATION OF ULTRADEFORMABLE SOFT ELASTIC NANO VESICLE ETHOSOMES FOR DERMAL DELIVERY

    Samnani, Ajay; Shahwal, Vimal; Bhowmick, Mithun; Joshi, Amit; Dubey, B. K.

    2012-01-01

    Clotrimazole is an antifungal drug for treatment of cutaneous candidiasis infections. However its oral administration is associated with number of drawbacks. The goal of the current investigation is to evaluate the transdermal potential of novel vesicular carrier, ethosomes, bearing Clotrimazole an antifungal having limited transdermal permeation. Clotrimazole loaded ethosomes were prepared, optimized and characterized for vesicular shape and surface morphology, vesicular size, size distribut...

  9. [New antifungal agents: voriconazole and caspofungin].

    Dupont, B

    2003-12-01

    Among new available antifungal agents voriconazole is a new triazole with an intravenous (i.v.) and oral formulation, and caspofungin is an echinocandin, new family with a new mode of action on the cell wall. It is available as an i.v. preparation. Both drugs have a broad spectrum targeting most of the usual pathogens: Candida and Aspergillus, even with low suceptibility or resistance to other antifungals. Voriconazole is also active on Scedosporium and Fusarium. The efficacy of these molecules was established in vitro and in experimental infections in animals either normal or immunosuppressed. Voriconazole is active in oropharyngeal and esophageal candidiasis, in refractory invasive candidiasis and as a first line treatment of invasive aspergillosis with better results than amphotéricine B. It was also effective in scedosporiosis and in fusariosis. Caspofungin is active in oropharyngeal and esophageal candidiasis, in invasive candidiasis ranking among the best drugs in non neutropenic patients. It was shown effective in refractory aspergillosis. As empirical treatment of febrile neutropenic patients, these molecules should probably be restricted to the highest risk-population. Safety is good, side effects are a rare cause of discontinuation of treatment, class specific drug-drug interactions occur with voriconazole. These molecules open an important field of investigations with combination of antifungal agents. PMID:15022787

  10. Traditional health practitioners' perceptions, herbal treatment and management of HIV and related opportunistic infections

    D. Davids; T. Blouws; O. Aboyade; D. Gibson; J.T. de Jong; C. van 't Klooster; G. Hughes

    2014-01-01

    Background: In South Africa, traditional health practitioners? (THPs) explanatory frameworks concerning illness aetiologies are much researched. However there is a gap in the literature on how THPs understand HIV-related opportunistic infections (OIs), i.e. tuberculosis, candidiasis and herpes zoste

  11. Candida albicans-associated necrotizing vasculitis producing life-threatening gastrointestinal hemorrhage.

    Sargent, Jeremy

    2012-02-01

    Patients undergoing treatment of acute lymphoblastic leukemia are at risk for fungal infections including disseminated candidiasis. We describe a case of systemic Candida albicans infection associated with life-threatening gastrointestinal hemorrhage due to unusual necrotizing vasculitis involving the gastrointestinal tract. We explore the association between Candida and such vasculopathy.

  12. New antifungal agents for the treatment of candidaemia

    Muñoz, Patricia; Guinea, Jesus; Rojas, Loreto; Bouza, Emilio

    2010-01-01

    Abstract Suspected or proven invasive candidiasis is an important indication for antifungal drugs and a leading cause of death. Prompt initiation of effective therapy has a marked effect on survival, but the indiscriminate application of different risk factor-based prediction models is massively increasing the number of patients treated unnecessarily. Fluconazole resistance levels are

  13. Plasma concentrations of caspofungin at two different dosage regimens in a patient with hepatic dysfunction

    van der Elst, K. C. M.; Bruggemann, R. J. M.; Rodgers, M. G. G.; Alffenaar, J. W. C.

    2012-01-01

    The currently recommended dosage regimen of caspofungin (50 mg/day) was developed for patients with invasive candidiasis. With invasive aspergillosis, successful outcomes occur in less than half the patients. We evaluate the pharmacokinetics in a patient with elevated liver enzyme levels after liver

  14. Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.

    Fiss, E.; Buckley, H R

    1987-01-01

    Actin was purified from Candida albicans cells by affinity chromatography by DNase-Sepharose and was recognized by immunoblotting with monoclonal antibody directed against chick muscle actin. The C. albicans 48-kilodalton protein recognized by sera from patients with invasive candidiasis was shown by DEAE chromatography and immunoblotting not to be identical with the purified C. albicans actin.

  15. Epidemiology and antifungal susceptibilities of yeasts causing vulvovaginitis in a teaching hospital.

    Gamarra, Soledad; Morano, Susana; Dudiuk, Catiana; Mancilla, Estefanía; Nardin, María Elena; de Los Angeles Méndez, Emilce; Garcia-Effron, Guillermo

    2014-10-01

    Vulvovaginal candidiasis is one of the most common mycosis. However, the information about antifungal susceptibilities of the yeasts causing this infection is scant. We studied 121 yeasts isolated from 118 patients with vulvovaginal candidiasis. The isolates were identified by phenotypic and molecular methods, including four phenotypic methods described to differentiate Candida albicans from C. dubliniensis. Antifungal susceptibility testing was performed according to CLSI documents M27A3 and M27S4 using the drugs available as treatment option in the hospital. Diabetes, any antibacterial and amoxicillin treatment were statistically linked with vulvovaginal candidiasis, while oral contraceptives were not considered a risk factor. Previous azole-based over-the-counter antifungal treatment was statistically associated with non-C.albicans yeasts infections. The most common isolated yeast species was C. albicans (85.2 %) followed by C. glabrata (5 %), Saccharomyces cerevisiae (3.3 %), and C. dubliniensis (2.5 %). Fluconazole- and itraconazole-reduced susceptibility was observed in ten and in only one C. albicans strains, respectively. All the C. glabrata isolates showed low fluconazole MICs. Clotrimazole showed excellent potency against all but seven isolates (three C. glabrata, two S. cerevisiae, one C. albicans and one Picchia anomala). Any of the strains showed nystatin reduced susceptibility. On the other hand, terbinafine was the less potent drug. Antifungal resistance is still a rare phenomenon supporting the use of azole antifungals as empirical treatment of vulvovaginal candidiasis. PMID:25005365

  16. Studies of Immune Responses in Candida vaginitis

    De Bernardis, Flavia; Arancia, Silvia; Sandini, Silvia; Graziani, Sofia; Norelli, Sandro

    2015-01-01

    The widespread occurrence of vaginal candidiasis and the development of resistance against anti-fungal agents has stimulated interest in understanding the pathogenesis of this disease. The aim of our work was to characterize, in an animal model of vaginal candidiasis, the mechanisms that play a role in the induction of mucosal immunity against C. albicans and the interaction between innate and adaptive immunity. Our studies evidenced the elicitation of cell-mediated immunity (CMIs) and antibody (Abs)-mediated immunity with a Th1 protective immunity. An immune response of this magnitude in the vagina was very encouraging to identify the proper targets for new strategies for vaccination or immunotherapy of vaginal candidiasis. Overall, our data provide clear evidence that it is possible to prevent C. albicans vaginal infection by active intravaginal immunization with aspartyl proteinase expressed as recombinant protein. This opens the way to a modality for anti-Candida protection at the mucosa. The recombinant protein Sap2 was assembled with virosomes, and a vaccine PEVION7 (PEV7) was obtained. The results have given evidence that the vaccine, constituted of virosomes and Secretory aspartyl proteinase 2 (Sap2) (PEV7), has an encouraging therapeutic potential for the treatment of recurrent vulvovaginal candidiasis. PMID:26473934

  17. Candida lusitaniae as an Unusual Cause of Recurrent Vaginitis and its Successful Treatment With Intravaginal Boric Acid

    Silverman, Neil S.; Margie Morgan; Nichols, W. S.

    2001-01-01

    Increasing use of short-course antifungal therapies in patients with recurrent vulvovaginitis may enable the emergence of less-common, more resistant yeast strains as vaginal pathogens. We report the case of a patient with chronically symptomatic and repeatedly treated vaginal candidiasis whose infection was attributable to Candida lusitaniae, a previously unreported cause of candidal vaginitis .

  18. Comparison of Fluconazole and Clotrimazole in the Treatment of Acute Candida Albicans Vulvovaginitis

    Fatemeh Bahadori

    2008-12-01

    Full Text Available Objective: This study compared two antifungal drugs, fluconazole and clotrimazole for the treatment of vulvo vaginal candidiasis. Materials and Methods: This randomized clinical trial was conducted on 120 women with vulvo vaginal candidiasis during a six month period. All patients answered a standard questionnaire containing questions about symptoms of vulvovaginal candidiasis and presence of vaginal discharge and signs of vulvar and vaginal inflammation were documented according to physical examination. Two swabs of vaginal discharge were obtained for each woman, one for direct smear, another for culture. The culture medium was Sabouraud Dextrose Agar (SDA. Patients were randomized into two groups of clotrimazole (vaginal cream for 7 days and 150 mg fluconazole in a single dose. Clinical and paraclinical responses were calculated.Results: Clinical improvement occurred in 96 cases (80%. This value was 86.7% and 73.3%for clotrimazole and fluconazole, respectively (P-value=0.04. Paraclinical response on tenth day of treatment was observed in 87 patients (72.5%. This value was 66.7% and 78.3%for clotrimazole and fluconazole groups, respectively (P-value=0.110. Mean days of treatment was 4.06 ±1.30 days for clotrimazole and 2.70 ±0.78 days for fluconazole (p value =0.031.Conclusion: Most of the clinical and paraclinical responses to the drugs used for the treatment of vulvo vaginal candidiasis are in the favour of fluconazole.

  19. Nutrition and Its Relationship to Autism.

    Adams, Lynn; Conn, Susan

    1997-01-01

    Discusses the relationship between food allergies and sensitivities and autism. Information is provided on two dietary problems (candidiasis and gluten/casein intolerance) and case histories of two three-year-old children with autism are provided to illustrate each of the problems. Diet and vitamin therapy interventions are also described.…

  20. Sexually transmitted infections and reproductive tract infections in female sex workers

    Shethwala Nimisha

    2009-04-01

    Full Text Available The present study was conducted in 300 female sex workers (FSWs from Surat city in 2005-2006. Vaginal swabs, endocervical swabs and serum samples were collected from each of these FSWs. Vaginal samples were screened for bacterial vaginosis (BV, candidiasis and Trichomonas vaginalis . Endocervical swabs were screened for gonococcal infection. Serological tests for human immunodeficiency virus (HIV, hepatitis B surface antigen (HBsAg and syphilis were performed. From a total of 300 FSWs, BV was detected in 40 (13.33%, trichomoniasis in six (2%, candidiasis in 31 (10.33%, HIV seropositivity in 35 (11.66%, HBsAg reactivity in 10 (3.33% and rapid plasma regain (RPR reactivity in 20 (6.66% cases. RPR-positive serum samples were confirmed by the treponema pallidum hemaglutination test. Gonococcal infection was not found in any of the FSWs. Of the total of 35 HIV-positive patients, 20 patients had associated coinfection. Of the 35 HIV-seropositive FSWs, BV was detected in six (17.14%, candidiasis in six (17.14%, syphilis in five (14.28% and HBsAg in two (5.71%. One (2.85% HIV-positive FSW was positive for both candidiasis and syphilis.

  1. The Candida albicans CNH1 gene encodes an antiporter important for potassium and pH homeostasis

    Zimmermannová, Olga; Sychrová, Hana

    Washington, DC : ASM, 2006. s. 50-50. ISBN 1-55581-382-8. [8th ASM Conference on Candida and Candidiasis. 13.03.2006-17.03.2006, Denver] R&D Projects: GA MŠk(CZ) LC531 Keywords : Candida albicans * Na+,K+/H+ antiporter * potassium homeostasis * intracellular pH Subject RIV: EB - Genetics ; Molecular Biology

  2. ANTİFUNGALLERİN SİSTEMİK MANTAR İNFEKSİYONLARINDA KULLANIMI VE DUYARLILIK DENEYLERİ: GENEL YÖNLENDİRME

    YÜCEL, AYHAN; KANTARCIOĞLU, A. Serda

    2014-01-01

    Background.- This paper outlines major distinctive features of conventional antifungal agents and their common practice, and also reviews various reports related to systemic antifungal prophylaxis and therapy administrations. Furthermore, general therapeutic approaches in some special situations such as certain types of candidiasis, aspergillosis, cryptococcosis, pregnancy, and nosocomial mycoses are summarized. The significance of susceptibility tests in antifungal therapy is discussed.* Ana...

  3. Interaktion mellem warfarin og oral miconazol-gel

    Ogard, C G; Vestergaard, Henrik

    2000-01-01

    We report a case of a 76 year-old woman who had been taking warfarin for seven years because of relapsing deep venous thrombosis. Her daily maintenance dose was 5 mg. Monthly measurements of international normalised ratio (INR) were stable between 2-3. She developed oral candidiasis and miconazol...

  4. Clinical Validation of the Analysis of Fluconazole in Oral Fluid in Hospitalized Children

    van der Elst, Kim C. M.; van Alst, Manouche; Lub-de Hooge, Marjolijn N.; van Hateren, Kai; Kosterink, Jos G. W.; Alffenaar, Jan-Willem C.; Scholvinck, Elisabeth H.

    2014-01-01

    Fluconazole is a first-line antifungal agent for the treatment and prophylaxis of invasive candidiasis in pediatric patients. Pediatric patients are at risk of suboptimal drug exposure, due to developmental changes in gastrointestinal and renal function, metabolic capacity, and volume of distributio

  5. Gallium-67 imaging in candidal esophagitis

    Rundback, J.H.; Goldfarb, C.R.; Ongseng, F. (Beth Israel Medical Center, New York, NY (USA))

    1990-01-01

    Ga-67 scanning has been used to evaluate esophageal carcinoma. It has demonstrated candidal infection in other body sites and, in one previous case, in the esophagus. The authors present a case of diffuse esophageal uptake of Ga-67 in esophageal candidiasis.

  6. Oat cell carcinoma of the esophagus: Unusual radiological appearances

    Primary oat cell carcinoma of the esophagus is a very rare tumour. The radiographic appearance of the three cases described in this paper are unusual because they resemble benign lesions such as leiomyoma, fibrous polyp and candidiasis. It would be interesting to investigate whether such an unusual appearance is common for this neoplasm. (orig.)

  7. Gallium-67 imaging in candidal esophagitis

    Ga-67 scanning has been used to evaluate esophageal carcinoma. It has demonstrated candidal infection in other body sites and, in one previous case, in the esophagus. The authors present a case of diffuse esophageal uptake of Ga-67 in esophageal candidiasis

  8. Oat cell carcinoma of the esophagus: Unusual radiological appearances

    Bedi, D.G.; Shaw, M.T.

    1986-08-01

    Primary oat cell carcinoma of the esophagus is a very rare tumour. The radiographic appearance of the three cases described in this paper are unusual because they resemble benign lesions such as leiomyoma, fibrous polyp and candidiasis. It would be interesting to investigate whether such an unusual appearance is common for this neoplasm.

  9. EXPERIENCE IN TREATING SECONDARY SYSTEMIC MYCOTIC INFECTION AFTER SEVERE BURNS ASSOCIATED WITH ELECTRIC INJURY

    谢卫兴; 李秀芝

    1995-01-01

    One patient with wound surface sepsis caused by secondary pyocyanic infection after extensive burns associated with visceral injuries (peptic ulcer hemorrhage, renal insufficiency and hepatic dysfunction) and generalized candidiasis albicans was cured after anti-infection treatment with proper antibiotics, removal of the infected focus, and effective anti-fungal drugs.

  10. CXCR1-mediated neutrophil degranulation and fungal killing promote Candida clearance and host survival.

    Swamydas, Muthulekha; Gao, Ji-Liang; Break, Timothy J; Johnson, Melissa D; Jaeger, Martin; Rodriguez, Carlos A; Lim, Jean K; Green, Nathaniel M; Collar, Amanda L; Fischer, Brett G; Lee, Chyi-Chia Richard; Perfect, John R; Alexander, Barbara D; Kullberg, Bart-Jan; Netea, Mihai G; Murphy, Philip M; Lionakis, Michail S

    2016-01-20

    Systemic Candida albicans infection causes high morbidity and mortality and is now the leading cause of nosocomial bloodstream infection in the United States. Neutropenia is a major risk factor for poor outcome in infected patients; however, the molecular factors that mediate neutrophil trafficking and effector function during infection are poorly defined. Using a mouse model of systemic candidiasis, we found that the neutrophil-selective CXC chemokine receptor Cxcr1 and its ligand, Cxcl5, are highly induced in the Candida-infected kidney, the target organ in the model. To investigate the role of Cxcr1 in antifungal host defense in vivo, we generated Cxcr1(-/-) mice and analyzed their immune response to Candida. Mice lacking Cxcr1 exhibited decreased survival with enhanced Candida growth in the kidney and renal failure. Increased susceptibility of Cxcr1(-/-) mice to systemic candidiasis was not due to impaired neutrophil trafficking from the blood into the infected kidney but was the result of defective killing of the fungus by neutrophils that exhibited a cell-intrinsic decrease in degranulation. In humans, the mutant CXCR1 allele CXCR1-T276 results in impaired neutrophil degranulation and fungal killing and was associated with increased risk of disseminated candidiasis in infected patients. Together, our data demonstrate a biological function for mouse Cxcr1 in vivo and indicate that CXCR1-dependent neutrophil effector function is a critical innate protective mechanism of fungal clearance and host survival in systemic candidiasis. PMID:26791948

  11. CX3CR1-dependent renal macrophage survival promotes Candida control and host survival

    Lionakis, M.S.; Swamydas, M.; Fischer, B.G.; Plantinga, T.S.; Johnson, M.D.; Jaeger, M.; Green, N.M.; Masedunskas, A.; Weigert, R.; Mikelis, C.; Wan, W.; Lee, C.C.; Lim, J.K.; Rivollier, A.; Yang, J.C.; Laird, G.M.; Wheeler, R.T.; Alexander, B.D.; Perfect, J.R.; Gao, J.L.; Kullberg, B.J.; Netea, M.G.; Murphy, P.M.

    2013-01-01

    Systemic Candida albicans infection causes high morbidity and mortality and is associated with neutropenia; however, the roles of other innate immune cells in pathogenesis are poorly defined. Here, using a mouse model of systemic candidiasis, we found that resident macrophages accumulated in the kid

  12. Card9 Mediates Intestinal Epithelial Cell Restitution, T-Helper 17 Responses, and Control of Bacterial Infection in Mice

    Sokol, Harry; Conway, Kara L.; Zhang, Mei; Choi, Myunghwan; Morin, Bret; Cao, Zhifang; Villablanca, Eduardo J.; Li, Chun; Wijmenga, Cisca; Yun, Seok Hyun; Shi, Hai Ning; Xavier, Ramnik J.

    2013-01-01

    BACKGROUND & AIMS: Caspase recruitment domain 9 (CARD9) is an adaptor protein that integrates signals downstream of pattern recognition receptors. CARD9 has been associated with autoinflammatory disorders, and loss-of-function mutations have been associated with chronic mucocutaneous candidiasis, bu

  13. Mouth and dental disorders

    C. de Baat; I. van der Waal

    2009-01-01

    Summary This chapter contains sections titled: • Introduction • Periodontal disease • Dental caries • Odontogenic infections • Alveolar osteitis • Xerostomia and hyposalivation • Candidiasis • Angular cheilitis • Denture stomatitis • Burning mouth syndrome • Recurrent aphthous stomatitis • Recurrent

  14. Comparative analysis of Gram’s method and PAS for the identification of Candida spp. samples from the oral mucosa

    Cátia Martins Leite Padilha

    2014-10-01

    Full Text Available Introduction: Candida species are part of the normal microbiota of healthy subjects, living as commensals. However, they can become pathogenic when changes in the mechanisms of host defense or disruption of anatomic barriers occur. Candidiasis is the most common fungal infection in the oral cavity, mainly caused by Candida albicans. The diagnosis is based on symptoms and clinical aspects, in association with laboratory methods. Objective: To evaluate the sensitivity and specificity of Gram’s method for Candida spp. identification in scrapes from the buccal mucosa and evaluate the degree of concordance between clinical and cytological methods in the diagnosis of oral candidiasis. Material and methods: A blind study was performed in 170 smears from patients of Hospital Universitário Antônio Pedro of Universidade Federal Fluminense (HUAP/UFF, stained by Gram (n = 57, periodic acid-Schiff (PAS (n = 57 and Papanicolaou (Pap (n = 57 methods. Results: The comparative analysis of the methods demonstrated a higher prevalence of Candida spp. (12% in PAS than in Gram staining, without statistic significance. The cytology method was positive in 93% of the clinical diagnosis of candidiasis. Conclusion: Gram was an adequate method; however more intensive professional training would be necessary to identify the fungus morphological structures. Although Pap test is the most common method of routine cytopathologic examination, for candidiasis diagnosis PAS staining is also recommended. Thus, it is suggested that candidiasis diagnosis should be accomplished by clinical evaluation in association with cytopathological analysis based on the identification of hyphae and/or pseudohyphae.

  15. Relationship between contraceptive method and vaginal flora.

    Peddie, B A; Bishop, V; Bailey, R R; McGill, H

    1984-08-01

    This study was undertaken to assess whether the vaginal flora was affected by the method of contraception, and in particular as to whether the incidence of vaginal candidiasis increased when oral contraceptives were used. One thousand and two consecutive vaginal or cervical swabs from women attending a family planning centre were cultured. Candida albicans was isolated from 13% of women using no contraception, 16% using oral contraceptives, and from 9%, 19% and 18% of those using diaphragms, intrauterine contraceptive devices (IUCD) and condoms respectively. These differences were not statistically significant. Women using an IUCD had significantly more Gram-positive cocci cultured than women in any other group, while those using diaphragms had significantly more Gram-negative bacilli. Our clinical impression that the use of oral contraceptives led to an increase in vaginal candidiasis, was not confirmed by this study. PMID:6596089

  16. In vitro effects of glycyrrhetinic acid on the growth of clinical isolates of Candida albicans.

    Pellati, Donatella; Fiore, Cristina; Armanini, Decio; Rassu, Mario; Bertoloni, Giulio

    2009-04-01

    Compounds derived from Glycyrrhiza glabra L. root have been used widely for centuries for their numerous therapeutic properties. The present study aimed to test the in vitro activity against Candida albicans strains of the compound 18-beta glycyrrhetinic acid (18-beta GA), derived from the root of Glycyrrhiza species. This antimicrobial activity was assessed using the National Committee for Clinical Laboratory Standards (NCCLS) method on C. albicans strains that were isolated from patients with recurrent vulvovaginal candidiasis (RVVC). The in vitro growth of the C. albicans strains was markedly reduced, in a pH-dependent manner, by relatively low doses (6.2 microg/mL) of 18-beta GA. The results demonstrate that 18-beta GA is a promising biological alternative for the topical treatment of recurrent vulvovaginal candidiasis (RVVC). PMID:19067381

  17. Oral ulcer as primary manifestation of HIV infection in an 80-year-old man

    Ludvigsen, Lene Ugilt Pagter; Søgaard, Ole Schmeltz

    Oral lesions such as candidiasis, Kaposi's sarcoma, hairy leukoplakia, herpes simplex infection, and ulcerative periodontitis are associated with HIV infection and may be the primary presentation in persons with undiagnosed HIV. We report a clinical case in which an 80-year-old man presented with...... an oral ulcer with morphological signs of immuno-suppression. The patient was perceived to be at low risk of HIV infection and thus, diagnostic HIV testing was delayed until subsequent development of Kaposi's sarcoma on the skin.......Oral lesions such as candidiasis, Kaposi's sarcoma, hairy leukoplakia, herpes simplex infection, and ulcerative periodontitis are associated with HIV infection and may be the primary presentation in persons with undiagnosed HIV. We report a clinical case in which an 80-year-old man presented with...

  18. Skin Immunity to Candida albicans.

    Kashem, Sakeen W; Kaplan, Daniel H

    2016-07-01

    Candida albicans is a dimorphic commensal fungus that colonizes healthy human skin, mucosa, and the reproductive tract. C. albicans is also a predominantly opportunistic fungal pathogen, leading to disease manifestations such as disseminated candidiasis and chronic mucocutaneous candidiasis (CMC). The differing host susceptibilities for the sites of C. albicans infection have revealed tissue compartmentalization with tailoring of immune responses based on the site of infection. Furthermore, extensive studies of host genetics in rare cases of CMC have identified conserved genetic pathways involved in immune recognition and the response to the extracellular pathogen. We focus here on human and mouse skin as a site of C. albicans infection, and we review established and newly discovered insights into the cellular pathways that promote cutaneous antifungal immunity. PMID:27178391

  19. The Absence of N-Acetyl-D-glucosamine Causes Attenuation of Virulence of Candida albicans upon Interaction with Vaginal Epithelial Cells In Vitro.

    Manczinger, Máté; Bocsik, Alexandra; Kocsis, Gabriella F; Vörös, Andrea; Hegedűs, Zoltán; Ördögh, Lilla; Kondorosi, Éva; Marton, Annamária; Vízler, Csaba; Tubak, Vilmos; Deli, Mária; Kemény, Lajos; Nagy, István; Lakatos, Lóránt

    2015-01-01

    To better understand the molecular events underlying vulvovaginal candidiasis, we established an in vitro system. Immortalized vaginal epithelial cells were infected with live, yeast form C. albicans and C. albicans cultured in the same medium without vaginal epithelial cells were used as control. In both cases a yeast to hyphae transition was robustly induced. Whole transcriptome sequencing was used to identify specific gene expression changes in C. albicans. Numerous genes leading to a yeast to hyphae transition and hyphae specific genes were upregulated in the control hyphae and the hyphae in response to vaginal epithelial cells. Strikingly, the GlcNAc pathway was exclusively triggered by vaginal epithelial cells. Functional analysis in our in vitro system revealed that the GlcNAc biosynthesis is involved in the adherence to, and the ability to kill, vaginal epithelial cells in vitro, thus indicating the key role for this pathway in the virulence of C. albicans upon vulvovaginal candidiasis. PMID:26366412

  20. Fungicidal response of a novel natural photosensitizer (Beta vulgaris) on Candida albicans with low-power laser radiation

    Mittal, Subhangi; Roy, Sukhdev; Srivastava, J. N.

    2013-05-01

    We report the efficacy of an aqueous extract of Beta vulgaris as a novel, natural photosensitizer for use in photodynamic therapy against Candidiasis disease. This study evaluates the effect of different laser wavelengths (He-Ne: 633 nm, Nd-YAG: 532 nm), power (17, 27 mW) and duration of exposure (5, 10, 15 min) in combination with the Beta vulgaris natural photosensitizer on the viability of Candida albicans causing Candidiasis disease. Although inhibition was observed in all cases, a maximum of 51.91% inhibition takes place with the combination of Beta vulgaris exposed to 532 nm at 27 mW for 15 min by the Agar well diffusion method. The study is important in optimizing different parameters and designing a low-power, compact, non-invasive and portable device for treatment.