Premnalatifolin A (1), a unique icetexane diterpene dimer was isolated from the stem-bark of Indian medicinal plant, Premna latifolia. Its structure and relative stereochemistry were elucidated on the basis of detailed spectroscopic analysis, including HRESIMS and 2D NMR (COSY, HSQC, HMBC, and NOESY) spectra. The compound has dimeric carbon skeleton composed of two icetexane skeletal diterpenes linked via ether bond. Further, premnalatifolin A (1) was also evaluated for its cytotoxicity against cancer cell lines (HT-29, A-431, MCF-7, Hep-G2, PC-3, A-549, B-16 F10, and ACHN), which displayed potent activity against HT-29 and MCF-7 cell lines with the IC50 values of 12.15 and 1.11mg/mL, respectively.
Plant secondary chemistry is determined by both genetic and environmental factors, and while large intraspecific variation in secondary chemistry has been reported frequently, the levels of genetic variation of many secondary metabolites in forest trees in the context of potential resistance against pests have been rarely investigated. We examined the effect of tree genotype and environment/site on the variation in defensive secondary chemistry of lodgepole pine, Pinus contorta var. latifolia, against the fungus, Grosmannia clavigera (formerly known as Ophiostoma clavigerum), associated with the mountain pine beetle, Dendroctonus ponderosae. Terpenoids were analyzed in phloem samples from 887, 20-yr-old trees originating from 45 half-sibling families planted at two sites. Samples were coll...
Methane (CH{sub 4}) produced microbially in sediments of marshes is emitted to the atmosphere primarily by flowing through and out of emergent aquatic plants. The magnitude of such emission rates and factors controlling those rates are not well understood. We evaluated CH{sub 4} emission from the widely distributed aquatic emergent plant cattail (Typha latifolia L.) in several wetlands in the United States using a field gas-exchange system that concurrently estimated stomatal aperture (i.e., conductance) on the surface of leaves and net photosynthesis. We compared gas exchange among plants of different age and from sites with different soil and atmospheric conditions. The mean rate of CH{sub 4} emission was 0.22{mu}mol m{sup -2} [leaf] s{sup -1}, which is 940 mg CH{sub 4} m{sup -2} d{sup -1} on a ground-area basis, with individual rates ranging from 0.01 to 1.49 {mu}mol m{sup -2} [leaf] s{sup -1}. For individual plants, we found emission rates were (i) highest for ...