c-reactive protein collaborates: Topics by WorldWideScience.org

Associations between plasma insulin-like growth factor-I and the markers of inflammation interleukin 6, C-reactive protein and YKL-40 in an elderly background population

Objective:The identification of biological markers in order to assess different aspects of COPD is an area of growing interest. The objective of this study was to investigate whether...Full Text Available

2011-01-01

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Early spondyloarthropathy: scintigraphic, biological, and clinical findings in MRI-positive patients

The objective of the present study was to test the hypothesis that circulating levels of insulin-like growth factor-I (IGF-I) are inversely associated with inflammatory processes in an elderly background population.

2010-01-01

4

British Library Electronic Table of Contents (United Kingdom)

There are no specific diagnostic tests or a gold standard method for measuring disease activity and outcome in spondyloarthropathies (SpA). Many different methods have been developed to assess the signs and symptoms in SpA. The aim of this study was to evaluate the value of scintigraphy, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and Bath Ankilosing Spondylitis Disease Activity Index (BASDAI) in the evaluation of disease activity in early axial SpA diagnosed with magnetic resonance imaging (MRI). Thirty early MRI-positive axial SpA patients (23 males, 7 females) with a median age of 35 (18?55) years and a median duration of inflammatory low back pain of 24 (8?60) months were included in the study. In the patients with sacroiliitis, the sensitivity, specificity, and pos...

2008-01-01

5

Abces du muscle iliopsoas : complication rare de la sacro-iliite a pyogene chez lenfant

British Library Electronic Table of Contents (United Kingdom)

Pyogenic sacroiliitis is a rare entity in children. Diagnosis is often delayed because of its variable clinical presentation, low suspicion by the examining physician, and rare findings on radiographs. Delayed diagnosis, however, results in complications such as iliopsoas abscess. We report the case of a 12-year-old girl hospitalized with a 21-day history of fever, pain in the left iliac fossa, and flexion contracture of the hip. On examination, she had fever (38.9 ^oC), psoitis, localized tenderness at the left sacroiliac joint, and pain elicited by lateral compression of the pelvis. The abdominal examination was normal. The erythrocyte sedimentation rate was 130mm in the first hour, C-reactive protein was 186mg/l, and the white blood cell count was 18,400/mm^3, with 79% neutrophils. Urin...

2010-01-01

6

Assessment of acute phase proteins and oxidative stress status of Nigerians using bleaching agents

Adiponectin concentrations increase during acute FFA elevation in humans treated with rosiglitazone.

The disruption of primary innate immune function of the epidermal layer of the skin accounts for the susceptibility of individuals using bleaching agents to localized or systemic infections. This subverted innate immunity in these people may lead to other pathological conditions. The resultant effects of skin bleaching and phagocytes activation in response to infections have not been studied in Nigerians using bleaching agents. The present study therefore assessed the levels of C-reactive protein (CRP), albumin, total antioxidant potential (TAP), total plasma peroxides (TPP), oxidative stress index (OSI) and malonaldehyde (MDA) in the users bleaching agents. Thirty (30) people who had used bleaching agents for average of 4.9 + 1.2 years participated in this study. They were recruited from various schools and markets within the city of Ibadan, Oyo State, Nigeria. Thirty apparently healthy staffs of University College Hospital Ibadan, Ibaadan, ...

7

The association of circulating inflammatory markers with recurrent vascular events after stroke: a prospective cohort study

The adipocytokine adiponectin is released by adipocytes upon activation of the peroxisome proliferator-activated receptor gamma (PPAR gamma). PPAR gamma has binding sites for thiazolidinediones and free fatty acids (FFAs). To evaluate if adiponectin serum concentrations are synergistically regulated by FFAs and thiazolidinediones IN VIVO plasma FFAs were acutely elevated in healthy subjects pre-treated with rosiglitazone or placebo. Sixteen healthy male subjects (23-37 years) were included in this double-blind, randomized, placebo-controlled parallel-group study. Rosiglitazone 8 mg or placebo was administered daily for 21 days. On the last day plasma FFA concentrations were increased by an intravenous triglyceride/heparin infusion. Blood for determination of adiponectin, C-reactive protein (CRP), leptin, resistin, FFAs, glucose, and insulin was drawn at baseline and on day 21 before and after 5 hours of triglyceride/heparin infusion. ...

2007-10-01

8

COLLABORATION AGREEMENT No. K1227

BackgroundInflammatory markers may be associated with recurrent vascular events after stroke. We aimed to: (1) determine the association between interleukin-6, C-reactive...Full Text Available

2011-01-01

9

COLLABORATION AGREEMENT No. K1225

Agreement to invite Project Associates

2006-01-01

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COLLABORATION AGREEMENT No. K 1690

Agreement to invite Project Associates

2005-01-01

11

COLLABORATION AGREEMENT No. K 1424

Agreement to invite Project Associates

2009-01-01

12

COLLABORATION AGREEMENT NO. K1577

Agreement to invite Project Associates

2007-01-01

13

Increasing the opportunities for UK-Canada collaboration

Agreement to invite Project Associates

2008-01-01

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DEVICE FOR TISSUE CULTURE, PROTEIN EXTRACTION AND PROTEIN CONCENTRATION

This paper outlines the opportunities for UK-Canada collaboration/feasibility studies in areas that include novel research into waste management and decommissioning. A number of Universities in the UK have programs relevant to such collaborations in areas such as fuels; thermal hydraulics, reactor system and materials.

2007-06-03

15

J-STORE (Japan)

Full Text Available

2007-12-28

16

Supporting Dynamic Ad hoc Collaboration Capabilities

Gearbox Reliability Collaborative Experimental Data Overview & Analysis (Presentation)

Modern HENP experiments such as CMS and Atlas involve as many as 2000 collaborators around the world. Collaborations this large will be unable to meet often enough to support working closely together. Many of the tools currently available for collaboration focus on heavy-weight applications such as videoconferencing tools. While these are important, there is a more basic need for tools that support connecting physicists to work together on an ad hoc or continuous basis. Tools that support the day-to-day connectivity and underlying needs of a group of collaborators are important for providing light-weight, non-intrusive, and flexible ways to work collaboratively. Some example tools include messaging, file-sharing, and shared plot viewers. An important component of the environment is a scalable underlying communication framework. In this paper we will describe our current progress on ...

2003-01-01

17

Collaborative systems and multiagent systems

This presentation details how wind turbine gearboxes fail to meet 20-year design life and how the Gearbox Reliability Collaborative (GRC) is addressing the challenges.

2010-05-01

18

Protein-protein interactions and structural analysis of citrus proteins involved in transcriptional control during citrus canker disease

This paper presents some basic elements regarding the domain of the collaborative systems, a domain of maximum actuality and also the multiagent systems, developed as a result of a sound study on the one-agent systems.

2009-01-01

19

Synchrotron resource of the Howard Hughes Medical Institute

No abstract prepared.

2010-07-01

20

Tensions of network security and collaborative work practice: understanding a single sign-on deployment in a regional hospital.

The Synchrotron Resource of the Howard Hughes Medical Institute will be dedicated to structural analyses of biological macromolecules, primarily through crystallography. This facility is under construction at the X4 port of the National Synchrotron Light Source at Brookhaven National Laboratory, and it will comprise three beamlines. The first line to be built has been devised to apply the multiwavelength anomalous diffraction method which provides direct estimates for the phases of reflections. The second line will be devoted to rapid and essentially routine diffraction measurements, mainly through the rotation method. These two experimental stations are contained within refrigerated radiation enclosures that will maintain clean environments, narrowly defined constant temperatures, and protection against biohazard at biosafety level BL-2. At the same time as the second line will be developed, the swath center is planned to first serve as a monitor of the white x-ray beam vertical ...

1989-07-01

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Putting the pieces together: endometriosis blogs, cognitive authority, and collaborative information behavior.

PMID:21398174

2011-03-12

22

Fresh-water biology, by Henry Baldwin Ward ... and George Chandler Whipple ... with the collaboration of a staff of ...

PMID:21464850

2011-04-01

23

COLLABORATION AGREEMENT No. K 1208 & K 1397

Language: English ...

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Scaffolding collaborative technical writing with procedural facilitation and synchronous discussion

Agreement to invite Project Associates

2005-01-01

25

British Library Electronic Table of Contents (United Kingdom)

With the advent of computer technology, researchers and instructors are attempting to devise computer support for effective collaborative technical writing. In this study, a computer-supported environment for collaborative technical writing was developed. This system (Process-Writing Wizard) provides process-oriented scaffolds and a synchronous online chat room to facilitate real-time collaborative writing practice. It allows multiple students to work synchronously on collaborative writing tasks via the Internet. It also helps develop collaborative writing strategies, such as creating team agendas, brainstorming, creating team outlines, and generating team articles. An experiment was conducted to examine the effect of the system on EFL (English as a Foreign Language) students? collaborativ...

2011-01-01

26

Heat shock proteins as emerging therapeutic targets

Effects of Collaborative Group Composition and Inquiry Instruction on Reasoning Gains and Achievement in Undergraduate Biology

Chaperones (stress proteins) are essential proteins to help the formation and maintenance of the proper conformation of other proteins and to promote cell survival after a large variety of environmental...Full Text Available

2005-11-01

27

Stability and Thermal Influences in Nonlinear Continuum ...

This study compared the effectiveness of collaborative group composition and instructional method on reasoning gains and achievement in college biology. Based on initial student reasoning ability (i.e.,...Full Text Available

2011-03-01

28

September 2008 - Permanent Representative of Austria to the United Nations Office and specialised institutions in Geneva, Ambassador C. Strohal, visiting the ATLAS control with Collaboration physicist M. Aleksa, accompanied by CMS Collaboration physicist F. Pauss and Physics Deputy Department Head M. Doser.

... The general two-phase Stefan problem with supercooling, superheating, and capillarity, was studied [T19] in collaboration ...

1990-11-01

29

CERN Multimedia

September 2008 - Permanent Representative of Austria to the United Nations Office and specialised institutions in Geneva, Ambassador C. Strohal, visiting the ATLAS control with Collaboration physicist M. Aleksa, accompanied by CMS Collaboration physicist F. Pauss and Physics Deputy Department Head M. Doser.

2008-01-01

30

CORIE: The First Decade of a Coastal-Margin Collaborative ...

Charged nanoparticles as protein delivery systems: A feasibility study using lysozyme as model protein

... geographically flexible rapid- deployment forecasting system that directly leverages the CORIE modeling and information management capabilities. ...

2006-09-01

31

The Stability and Formation of Native Proteins from Unfolded Monomers Is Increased through Interactions with Unrelated Proteins

Udgivelsesdato: 2008/5

2008-01-01

32

Localization of Reversion-Induced LIM Protein (RIL) in the Rat Central Nervous System

The intracellular concentration of protein may be as high as 400 mg per ml; thus it seems inevitable that within the cell, numerous protein-protein contacts are constantly occurring. A basic biochemical...Full Text Available

33

Understanding Groups' Properties as a Means of Improving Collaborative Search Systems

Reversion-induced LIM protein (RIL) is a member of the ALP (actinin-associated LIM protein) subfamily of the PDZ/LIM protein family. RIL serves as an adaptor protein and seems to regulate cytoskeletons....Full Text Available

2009-02-28

34

A data centered collaboration portal to support global carbon flux analysis

Understanding the similar properties of people involved in group search sessions has the potential to significantly improve collaborative search systems; such systems could be enhanced by information retrieval algorithms and user interface modifications that take advantage of important properties, for example by re-ordering search results using information from group members' combined user profiles. Understanding what makes group members similar can also assist with the identification of groups, which can be valuable for connecting users with others with whom they might undertake a collaborative search. In this workshop paper, we describe our current research efforts towards studying the properties of a variety of group types. We discuss properties of groups that may be relevant to designers of collaborative search systems, and propose ways in which understanding such properties could influence the design of interfaces and ...

2009-01-01

35

British Library Electronic Table of Contents (United Kingdom)

Abstract Carbon climate, like other environmental sciences, has been changing. Large scale synthesis studies are becoming more common. These synthesis studies are often conducted by science teams that are geographically distributed and on data sets that are global in scale. A broad array of collaboration and data analytics tools are now available that could support these science teams. However, building tools that scientists actually use is difficult. Also, moving scientists from an informal collaboration structure to one mediated by technology often exposes inconsistencies in the understanding of the rules of engagement between collaborators. We have developed a scientific collaboration portal, called fluxdata.org, which serves the community of scientists providing and analyzing the globa...

2010-01-01

36

Salt modulates the stability and lipid binding affinity of the adipocyte lipid-binding proteins

Protein misfolding disorders and macroautophagy

Adipocyte lipid-binding protein (ALBP or aP2) is an intracellular fatty acid-binding protein that is

2003-01-01

37

Misfolded Proteins and Retinal Dystrophies

A large group of diseases, termed protein misfolding disorders, share the common feature of the accumulation of misfolded proteins. The possibility of a common mechanism underlying either the pathogenesis...Full Text Available

2011-04-01

38

CAAX-box protein, prenylation process and carcinogenesis

Many mutations associated with retinal degeneration lead to the production of misfolded proteins by cells of the retina. Emerging evidence suggests that these abnormal proteins cause cell death...Full Text Available

2010-01-01

39

RIN4-like proteins mediate resistance protein-derived soybean defense against Pseudomonas syringae

CAAX proteins are widely involved in global cellular functions such as proliferation, differentiation, and carcinogenesis. As an important modulator of biological activity, signal transduction via protein...Full Text Available

40

Protein S inherited qualitative deficiency: novel mutations and phenotypic influence.

Resistance (R) protein mediated recognition of pathogen avirulence effectors triggers signaling that induces a very robust form of species-specific immunity in plants. The soybean Rpg1-b protein mediates...Full Text Available

2010-11-01

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Overcoming the challenges of membrane protein crystallography

PMID:20880255

2010-12-01

42

Effect of Garlic on the Digestion of Beef Protein during Storage

Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane...Full Text Available

2008-10-01

43

A modified immunoblot method to identify substrates of protein kinases

Full Text Available

1991-10-01

44

CONFLICT AND COLLABORATION

Full Text Available

45

British Library Electronic Table of Contents (United Kingdom)

The establishment of workable collaborative relations with indigenous elites formed a vital part of the maintenance of the British Empire. This was especially so in the Malay States, where the Malay rulers enjoyed considerable local prestige as religious leaders and as the organising principle of Malay society itself. As the case of Sultan Ibrahim of Johore demonstrates, nevertheless, indigenous collaborators were no mere puppets and enjoyed considerable freedom of action to frustrate and impede the policies of their imperial overlords. Sultan Ibrahim presents a particularly interesting case study as his 64-year reign more or less spanned the entire period of formal British rule in Malaya.

2008-01-01

46

Variations of endonasal anatomy: relevance for the endoscopic endonasal transsphenoidal approach

The I3I Model; Identifying Cultural Determinants of Information ...

BackgroundThe endoscopic endonasal transsphenoidal approach (EETA) to the pituitary is performed by ear, nose, and throat (ENT) surgeons in collaboration with neurosurgeons but also...Full Text Available

2010-06-01

47

Quantum chromodynamics with advanced computing

... Information behavior in dynamic group work contexts: Interwoven situational awareness, dense social networks and contested collaboration in ...

2009-06-01

48

Non-Intrusive, Laser-Based Imaging of Jet-A Fuel Injection and Combustion Species in High Pressure, Subsonic Flows

We survey results in lattice quantum chromodynamics from groups in the USQCD Collaboration. The main focus is on physics, but many aspects of the discussion are aimed at an audience of computational physicists.

2008-07-01

49

Knowledge Encapsulation Framework for Collaborative Social Modeling

The emphasis of combustion research efforts at NASA Glenn Research Center (GRC) is on collaborating

2000-01-01

50

Enterprise Command, Control, and Design: Bridging C2 ...

This paper describes the Knowledge Encapsulation Framework (KEF), a suite of tools to enable knowledge inputs (relevant, domain-specific facts) to modeling and simulation projects, as well as other domains that require effective collaborative workspaces for knowledge-based task. This framework can be used to capture evidence (e.g., trusted material such as journal articles and government reports), discover new evidence (covering both trusted and social media), enable discussions surrounding domain-specific topics and provide automatically generated semantic annotations for improved corpus investigation. The current KEF implementation is presented within a wiki environment, providing a simple but powerful collaborative space for team members to review, annotate, discuss and align evidence with their modeling frameworks. The novelty in this approach lies in the combination of automatically tagged and user-vetted resources, which increases user ...

2009-03-24

51

Collaborative Research and Development (CR&D). Delivery ...

... Information behavior in dynamic group work contexts: interwoven situational awareness, dense social networks and contested collaboration in ...

2011-05-14

52

An Advance in Superconducting Magnet Technology Opens the Door...

... 7 Page 11. Figure 5. Diffraction pattern shown a and ~ slip directions with the associated misalignment for single colonies of Ti6242 [2]. 8 Page 12. -- ...

2005-10-01

53

A - Z Index : Environmental Energy Technologies Division

magnet research at several national laboratories through its Advanced Accelerator Technology Program. The HEP Conductor Development Program, a collaboration among national...

2011-08-20

54

Collaborations in Mind, Brain, and Education: An Analysis of Researcher-Practitioner Partnerships in Three Elementary School Intervention Studies

Buildings Energy-Efficient Research Laboratories Energy Efficiency in Federal Facilities Energy Efficiency Standards Group Energy Efficient Windows Collaborative Energy &...

2011-07-15

55

British Library Electronic Table of Contents (United Kingdom)

A reciprocal relationship informing both research and practice is one of the primary goals in the emerging field of Mind, Brain, and Education (MBE), yet the field has little documentation and analysis of such collaborations. In this article, we present case studies of three research-practice partnerships taking place between scientists and educators. By comparing and contrasting these three partnerships we expect to illuminate common characteristics, challenges, and benefits that come with doing MBE work.

2011-01-01

56

Category III Chronic Prostatitis/Chronic Pelvic Pain Syndrome: Insights from The National Institutes of Health Chronic Prostatitis Collaborative Research Network Studies

Distinct spatial characteristics of industrial and public research collaborations: Evidence from the 5th EU Framework Programme

Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) remains an enigmatic medical condition. Creation of the (NIH) Chronic Prostatitis Collaborative Research Network (CPCRN) funded by...Full Text Available

2008-07-01

57

Kinetics of spherulite formation and growth: Salt and protein concentration dependence on proteins b-lactoglobulin and insulin

This study compares the spatial characteristics of industrial R&D networks to those of public research R&D networks (i.e. universities and research organisations). The objective is to measure the impact of geographical separation effects on the constitution of cross-region R&D collaborations for both types of collaboration. We use data on joint research projects funded by the 5th European Framework Programme (FP) to proxy cross-region collaborative activities. The study area is composed of 255 NUTS-2 regions that cover the EU-25 member states (excluding Malta and Cyprus) as well as Norway and Switzerland. We adopt spatial interaction models to analyse how the variation of cross-region industry and public research networks is affected by geography. The results of the spatial analysis provide evidence that geographical factors significantly affect patterns of industrial R&D collaboration, ...

2010-01-01

58

British Library Electronic Table of Contents (United Kingdom)

Proteins aggregated into spherulite structures of amyloid fibrils have been observed in patients with certain brain diseases such as Alzheimers and Parkinsons. The conditions under which these protein spherulites form and grow are not currently known. In order to illuminate the role of environmental factors on protein spherulites, this research aims to explore the kinetics and mechanisms of spherulite formation and growth, as monitored by optical microscopy, in a range of salt concentrations, and initial protein concentrations for two model proteins: bovine b-lactoglobulin and insulin. These two proteins are significantly different in their size and fibril growth rate, but both of these proteins have been shown previously to form amyloid fibrils and spherulites under low pH conditions. The...

2009-01-01

59

Whey protein isolate attenuates strength decline after eccentrically-induced muscle damage in healthy individuals

Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1

BackgroundWe examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals.MethodsSeventeen...Full Text Available

60

Thioredoxin Is an Essential Protein Induced by Multiple Stresses in Bacillus subtilis

Known vertebrate GATA proteins contain two zinc fingers and are required in development, whereas invertebrates express a class of essential proteins containing one GATA-type zinc finger. We isolated...Full Text Available

2001-04-02

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61

The protein storage vacuole

Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...Full Text Available

1998-04-01

62

The p14 FAST Protein of Reptilian Reovirus Increases Vesicular Stomatitis Virus Neuropathogenesis?

Storage proteins are deposited into protein storage vacuoles (PSVs) during plant seed development and maturation and stably accumulate to high levels; subsequently, during germination the storage...Full Text Available

2001-12-10

63

Strategies for the uses of lanthanide NMR shift probes in the determination of protein structure in solutio. Application to the EF calcium binding site of carp parvalbumin.

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

2009-01-01

64

Stage-specific protein synthesis during early embryogenesis in Drosophila melanogaster.

The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous...Full Text Available

1980-10-01

65

Serum protein binding and the role of increased alpha 1-acid glycoprotein in moderately obese male subjects.

The changes in protein species synthesized during early Drosophila embryogenesis were characterized by two-dimensional electrophoresis. Of the 261 proteins scored, 68 (26%) show dramatic changes in...Full Text Available

1983-01-01

66

Selecting Folded Proteins from a Library of Secondary Structural Elements

Serum protein and lipid concentrations as well as the serum protein binding of propranolol, diazepam and phenytoin were measured in normal weight and obese volunteers. Concentrations of alpha 1-acid...Full Text Available

1984-12-01

67

Regulation of the Actin Cytoskeleton by an Interaction of IQGAP Related Protein GAPA with Filamin and Cortexillin I

A protein evolution strategy is described by which double-stranded DNA fragments encoding defined E. coli protein secondary structural elements (α-helices, β-strands...Full Text Available

2008-01-09

68

Regulation of ROMK1 Channels by Protein-tyrosine Kinase and -tyrosine Phosphatase*

Filamin and Cortexillin are F-actin crosslinking proteins in Dictyostelium discoideum allowing actin filaments to form three-dimensional networks. GAPA, an IQGAP related protein, is...Full Text Available

69

Purification, characterization and binding interactions of the Chinese-cobra (Naja naja atra) serum antitoxic protein CSAP.

We have used the two-electrode voltage clamp technique and the patch clamp technique to investigate the regulation of ROMK1 channels by protein-tyrosine phosphatase (PTP) and protein-tyrosine...Full Text Available

2001-03-09

70

Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.

The characterization of the single-chain protein in Chinese-cobra (Naja naja atra) blood serum, which yields strong specific protection against the venom of the same snake, is reported. The protein,...Full Text Available

1993-07-15

71

Protein Damage by Reactive Electrophiles: Targets and Consequences

A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. Its N-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts...Full Text Available

1978-09-01

72

ProMoST: A tool for calculating the pI and molecular mass of phosphorylated and modified proteins on 2 dimensional gels

It has been sixty years since the Millers first described the covalent binding of carcinogens to tissue proteins. Protein covalent binding was gradually overshadowed by the emergence of DNA...Full Text Available

2008-01-01

73

Partial Characterization of Cadmium-Binding Protein from Roots of Tomato ¹

Protein modifications such as phosphorylation are often studied by two-dimensional gel electrophoresis since the perturbation in the protein’s pI value is readily detected by this method....Full Text Available

2009-01-01

74

PDBe: Protein Data Bank in Europe

Cd-binding protein was extracted from tomato roots and purified on QAE-Sephadex A-25 and on Sephadex G-75 in 1 molar KCl buffer. The protein preparation was light brown and contained predominantly Cd...Full Text Available

1986-07-01

75

Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging

The Protein Data Bank in Europe (PDBe; pdbe.org) is actively involved in managing the international archive of biomacromolecular structure data as one of the partners in the Worldwide Protein Data Bank...Full Text Available

2011-01-01

76

The nucleocapsid protein (NC) of retroviruses plays a major role in genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA binding. To further...Full Text Available

1998-03-01

77

NASCENT: An automatic protein interaction network generation tool for non-model organisms

The activity of Src-related protein-tyrosine kinases is repressed by the phosphorylation of a conserved carboxyl-terminal tyrosine by another cytoplasmic protein-tyrosine kinase termed p50csk. In this...Full Text Available

1994-05-24

78

Modular coherence of protein dynamics in yeast cell polarity system

Large quantity of reliable protein interaction data are available for model organisms in public depositories (e.g., MINT, DIP, HPRD, INTERACT). Most data correspond to experiments with the proteins...Full Text Available

79

Mammalian end binding proteins control persistent microtubule growth

In this study, we investigated on a systems level how complex protein interactions underlying cell polarity in yeast determine the dynamic association of proteins with the polar cortical domain (PCD)...Full Text Available

2011-05-03

80

How DNA coiling enhances target localization by proteins

End binding proteins (EBs) are highly conserved core components of microtubule plus-end tracking protein networks. Here we investigated the roles of the three mammalian EBs in controlling microtubule...Full Text Available

2009-03-09

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81

Expression and Characterization of the Mycobacterium tuberculosis Serine/Threonine Protein Kinase PknB

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

2008-10-14

82

Ethylene Regulates Monomeric GTP-Binding Protein Gene Expression and Activity in Arabidopsis1

PknB is a member of the newly discovered eukaryotic-like protein serine/threonine kinase (PSTK) family of proteins. The pknB gene was cloned and expressed in Escherichia coli....Full Text Available

1999-11-01

83

Effect of Protein Binding on the Pharmacological Activity of Highly Bound Antibiotics?

Ethylene rapidly and transiently up-regulates the activity of several monomeric GTP-binding proteins (monomeric G proteins) in leaves of Arabidopsis as determined by two-dimensional gel electrophoresis...Full Text Available

2003-04-01

84

Coat protein gene duplication in a filamentous RNA virus of plants.

During antibiotic drug development, media are frequently spiked with either serum/plasma or protein supplements to evaluate the effect of protein binding. Usually, previously reported serum or plasma...Full Text Available

2008-11-01

85

Cardiac Myosin Is a Substrate for Zipper-interacting Protein Kinase (ZIPK)*

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

1992-10-01

86

Biosynthesis and characterization of rabbit tooth enamel extracellular-matrix proteins.

Zipper-interacting protein kinase (ZIPK) is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain...Full Text Available

2010-02-19

87

Being a binding site: Characterizing residue composition of binding sites on proteins

Tooth enamel biomineralization is mediated by enamel proteins synthesized by ameloblast cells. Two classes of proteins have been described: enamelins and amelogenins. In lower vertebrates the absence...Full Text Available

1988-05-01

88

Analyzing the simplicial decomposition of spatial protein structures

The Protein Data Bank contains the description of more than 45,000 three-dimensional protein and nucleic-acid structures today. Started to exist as the computer-readable depository of crystallographic...Full Text Available

89

An Analysis of the Subunit Structure of the Crystalloid Protein Complex from Castor Bean Endosperm 1

BackgroundThe fast growing Protein Data Bank contains the three-dimensional description of more than 45000 protein- and nucleic-acid structures today. The large majority of the data...Full Text Available

90

Alpha-Hemoglobin-Stabilizing Protein: An Erythroid Molecular Chaperone

Chromatographic and electrophoretic studies have shown that the subunits of the crystalloid protein, isolated from mature castor bean (Ricinus communis L. cv Hale) seed endosperm protein...Full Text Available

1983-06-01

91

ppbar enhancement in B and J/Psi decay

Alpha-hemoglobin-stabilizing protein (AHSP) is an erythroid-specific protein that acts as a molecular chaperone for the free α chains of hemoglobin. Evidence strongly suggests...Full Text Available

2011-01-01

92

UK-China Bridge in Sustainable Energy and Built Environment

The near-threshold enhancement in the ppbar invariant mass spectrum from the B^+ -> K^+ ppbar decay reported recently by the BaBar Collaboration is studied within the J\\"ulich NNbar model. We illustrate that the invariant mass dependence of the ppbar spectrum close to the threshold can be reproduced by the final state interactions. This explanation is in line with our previous analysis of the ppbar invariant mass spectrum from the J/Psi -> gamma ppbar decay measured by the BES Collaboration. We also comment on a structure found recently in the pi^+ pi^- eta' mass spectrum of the radiative J/Psi decay by the BES Collaboration. In particular we argue that one should be rather cautions in bringing this structure in connection with the enhancement found in the ppbar invariant mass spectrum or with the existence of NNbar bound states.

2006-01-01

93

Online consumer communities, collaborative learning and innovation

ObjectivesThe primary aim is to enable China to address its energy and infrastructure issues in a sustainable manner, without hindering economic progress, with the UK benefiting from an enhanced uptake of its technology, knowledge and expertise through enhanced business opportunities and strengthened collaboration with key Chinese Institutions. ~%~~%~Objectives~%~1) To build capacity and capability in technology transfer and knowledge exploitation in Chinese and UK business and industry through practical, [continued...]DescriptionThe Chinese 11th Five-Year Plan considers Sustainable Energy Supply and Sustainable Built Environment as crucial for achieving sustainable development. Recognising the potential benefits, the UK government has actively encouraged international collaborations with China. Two Engineering Schools at Queen's University Belfast (QUB), with internationally recognised research excellence in the Built ...

2012-01-31

94

British Library Electronic Table of Contents (United Kingdom)

Purpose - The paper aims to outline the managerial challenges faced by the organizations interested in leveraging knowledge and creative talent embedded in online customers' communities to sustain innovation in b-2-c industries. Design/methodology/approach - Through a detailed case study analysis of a leading food producer who launched an online open collaborative platform to gather users' idea for new products the paper aims to highlight the transformational effort that firms have to make in order to leverage knowledge absorption from customers in the context of innovation. Findings - The paper suggests potential strategies for conventional companies to engage consumers in knowledge (co-creation) and collaborative innovation processes, formulating some hypothesis that could support an int...

2011-01-01

95

The structure of receptor-associated protein (RAP)

Phospholemman: A Novel Cardiac Stress Protein

The receptor-associated protein (RAP) is a molecular chaperone that binds tightly to certain newly synthesized LDL receptor family members in the endoplasmic reticulum (ER) and facilitates their delivery...Full Text Available

2007-08-01

96

PDBpaint, a visualization webservice to tag protein structures with sequence annotations

Phospholemman (PLM), a member of the FXYD family of regulators of ion transport, is a major sarcolemmal substrate for protein kinases A and C in cardiac and skeletal muscle. In the heart, PLM...Full Text Available

2010-08-01

97

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

2011-01-01

98

Male Reproductive Proteins and Reproductive Outcomes

Biogenesis of the Protein Storage Vacuole Crystalloid

Male reproductive proteins (MRPs), associated with sperm and semen, are the moieties responsible for carrying male genes into the next generation. Evolutionary biologists have focused on their...Full Text Available

2008-06-01

99

Alpha-1 antitrypsin is a potential biomarker for hepatitis B

We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric reporter...Full Text Available

2000-08-21

100

9902631 - Lysozyme - NASA

BackgroundFunction exertion of specific proteins are key factors in disease progression, thus the systematical identification of those specific proteins is a prerequisite to understand...Full Text Available

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101

WIZARD collaboration : Papers presented at the 24. International Cosmic Ray Conference, August 28-September 8, Rome 1995

Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

102

UV renormalons in QCD and their phenomenological implications

This report described the papers presented at the 24. International Cosmic Ray Conference held at Rome in 28 August - 8 September 1995

1995-09-01

103

The polymorphism architecture of mouse genetic resources elucidated using genome-wide resequencing data: implications for QTL discovery and systems genetics

I report on some recent work done in collaboration with E. de Rafael on the connection between ultraviolet renormalons in QCD and Nambu-Jona-Lasinio-like Lagrangians as its effective description at low energies. (orig.)

1998-05-01

104

The Limits of Collaboration: A Qualitative Study of Community Ethical Review of Environmental Health Research

Mouse genetic resources include inbred strains, recombinant inbred lines, chromosome substitution strains, heterogeneous stocks, and the Collaborative Cross (CC). These resources were generated through...Full Text Available

2007-07-01

105

Texas - Energy Collaborations Working for the Environment

Objectives. We assessed the effectiveness of various systems of community participation in ethical review of environmental health research.Methods. We used situation...Full Text Available

2009-08-01

106

THE ENVIRONMENTAL MANAGEMENT AND CO-ORDINATION ACT, 1999

This fact sheet highlights how renewable energy and energy efficiency technologies can and are being used to reduce air emissions and meet environmental goals, showcasing case studies and technology-specific topics.

2007-12-01

107

THE ANAEROBIC TREATMENT OF WASTES CONTAINING RECALCITRANT AND INHIBITORY COMPOUNDS

Subject to the provisions of the Radiation Protection Act, the Authority, on the advice of ...(f) in collaboration with the Radiation Protection Board, conduct an ionising radiation monitoring programme and ...or document kept under the control of the Radiation Protection Board.

108

Social Work Role in Pain Management with Hospice Caregivers: A National Survey

ObjectivesObjectives Not AvailableDescriptionA collaborative study of the anaerobic treatment of wastes containing recalcitrant and inhibitory compounds using the SERC Anaerobic facility, in particular biomass structure, monitoring and control, catabolism and toxicity, pre-acidification and microbial growth and mesophilic and thermophilic lignocellulose degradation.~%~

1995-01-20

109

Professions Australia - A national organisation of professional associations

This article reports on an exploratory study of hospice social workers’ assessment and collaborative practices related to pain management; especially caregiver concerns about patient...Full Text Available

2009-01-01

110

Panel > Cloudenomics 101 What Cloud Computing Really Means for

...to have a representative on the YPRT 2004 Integrated approaches to sustainability among the professions For ICT to innovate it must have collaboration with other Information and Technology related groups such as ACM, Australian Library and Information Association, Records Management Association, Archivists Association of Australia, ...

111

Microfungal contamination of damp buildings--examples of risk constructions and risk materials.

Aug 8, 2011 ... Panel > Cloudenomics 101 What Cloud Computing Really Means for NASA. 11:00 am in Collaboration, LiveCall by Lucas Cioffi (Moderator) ...

112

Information Sharing in the Era of WikiLeaks: Balancing Security and Collaboration.

To elucidate problems with microfungal infestation in indoor environments, a multidisciplinary collaborative pilot study, supported by a grant from the Danish Ministry of Housing and Urban Affairs,...Full Text Available

1999-06-01

113

Incorporating Patient Perspectives into the Personal Health Record: Implications for Care and Caring

As the IC Information Sharing Executive (IC ISE), my main focus today concerns classified information--and, in particular, information that is derived from intelligence sources and methods, or information that is reflected in the analytic judgments and as...

2011-01-01

114

Impact of Bt corn pollen on monarch butterfly populations: A risk assessment

Electronic personal health records (ePHRs) can potentially maximize access and coordination of health information and improve patient/clinician collaboration, patient self-management, and health outcomes....Full Text Available

115

Gene Signature For Predicting Survival Outcome Of Human Hepatocellular Carcinoma (HCC) - Technology Transfer Center

A collaborative research effort by scientists in several states and in Canada has produced information to develop a formal risk assessment of the impact of Bt corn on monarch butterfly...Full Text Available

2001-10-09

116

Establishing the Irish Critical Care Trials Group: 'who wins in battle makes many calculations before the battle is fought'

The National Cancer Institute Laboratory of Human Carcinogenesis is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize a gene signature for prognosis of hepatocellular carcinoma in patients.

117

Emotions run high in race for collider

Quality research, requiring large numbers of participants, in the intensive care unit (ICU) population requires multicentre collaboration. Although logistically challenging, this methodology reduces...Full Text Available

2008-01-01

118

CERN Multimedia

The head of KEK expressed his dismay that SLAC has entered into a collaboration with 3 other US labs and proposes to build the next linear collider at Fermilab, Ilinois. KEK wants the next accelerator to be built somewhere in the Asian Pacific region (1 page).

2001-01-01

119

Effects of Manufacturing Defects and Scaling on Glass Fiber Composite Sandwich Panels in General Aviation Aircraft Structures.

COST ESTIMATING SYSTEMS FOR REMEDIAL ACTION PROJECTS

This report documents the collaborative effort between the United States and Canada to investigate the impact of process-induced defects on strength and stiffness under static and fatigue loading and for low-cost composite aircraft structures, in particul...

2009-01-01

120

Approaches to enhancing the quality of drug therapy. A joint statement by the CMA and the Canadian Pharmaceutical Association. Canadian Medical Association.

This paper details the ongoing collaboration between the U.S. EPA and the U.S. Army Corps of Engineers in the development of complementary micro-computer based cost estimating systems for hazardous waste remediations. he U.S. EPA system, "Remedial Action Cost Estimating System" (...

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121

300 W polymer electrolyte fuel cell generators for educational purposes

This joint statement was developed by the CMA and the Canadian Pharmaceutical Association, a national association of pharmacists, and includes the goal of drug therapy, strategies for collaboration...Full Text Available

1996-09-15

122

Le LHC, un projet mondial

A 300 W fuel cell power pack has been developed for educational purposes in close collaboration with the Fachhochschule Solothurn Nordwestschweiz. The project was initiated and financed by the Swiss Federal Office of Energy. The outlay and the performance of the power pack are described. (author) 3 figs.

1999-08-01

123

CERN Multimedia

Since the mid-1980s the number of scientists from all over the world using CERN's facilities has increased enormously. Currently more than 6,000 users, over half of the planet's high-energy physicists, carry out fundamental research at CERN. This user community is living proof that CERN welcomes inter- regional collaboration which benefits all and boosts the progress of science. The LHC, the only machine capable of addressing problems way beyond today's frontiers of high energy physics, offers an unique opportunity for extending world wide collaboration.

1994-01-01

124

Investigating Biochar: from Source to Sink (Overview - Research)

Electromagnetic form factors of nucleons with QCD constraints: Systematic study of space- and time-like regions

...Investigating Biochar: from Source to Sink (Overview - Research) The Sustainable Agriculture Flagship is leading national collaborative research analysing the properties ...and potential of a variety of biochars to improve soil health and sequester carbon. The Sustainable Agriculture Flagship is leading national collaborative research ...analysing the properties and potential of a variety of biochars to improve soil health and sequester carbon. Biochar, sustainable agriculture, soil,...carbon, SAF, pyrolysis, fertiliser, biochar, soil types, greenhouse gas, herbicide, pesticide, bioenergy, soil science, carbon-rich solid, ...

125

Collaboration work for nuclear power building using 3D CAD information

Elastic electromagnetic form factors of nucleons are investigated for both the time-like and the space-like momenta by using the unsubtracted dispersion relation with QCD constraints. It is shown that the calculated form factors reproduce the experimental data reasonably well; they agree with recent experimental data for the neutron magnetic form factors for the space-like data obtained by the CLAS Collaboration and are compatible with the ratio of the electric and magnetic form factors for the time-like momentum obtained by the BABAR Collaboration.

2010-04-15

126

Collaboration work for nuclear power building using 3D CAD information

In the construction work for the Unit No.5 Turbine Building at Hamaoka Nuclear Power Station, the collaboration work for the planning and the coordination of the building construction and the machinery installation was carried out utilizing three-dimensional computer aided design (3D CAD) information. It was the first time in Japan for a building constructor and a plant manufacturer to work together, exchanging 3D CAD data of the whole building and adjusting the construction schedule. It was made possible to confirm the correspondence concerning the planning of both companies from the primary stage of the project by the total process simulation and to adjust on-going work precisely by the partial process simulation.

2006-02-15

127

Achievements in testing of the MGA and FRAM isotopic software codes under the DOE/NNSA-IRSN cooperation of gamma-ray isotopic measurement systems

In the construction work for the Unit No.5 Turbine Building at Hamaoka Nuclear Power Station, the collaboration work for the planning and the coordination of the building construction and the machinery installation was carried out utilizing three-dimensional computer aided design (3D CAD) information. It was the first time in Japan for a building constructor and a plant manufacturer to work together, exchanging 3D CAD data of the whole building and adjusting the construction schedule. It was made possible to confirm the correspondence concerning the planning of both companies from the primary stage of the project by the total process simulation and to adjust on-going work precisely by the partial process simulation.

2006-02-01

128

Thermal decomposition of gamma irradiated proteins by thermal analysis methods

DOE/NNSA and IRSN collaborated on a study of gamma-ray instruments and analysis methods used to perform isotopic measurements of special nuclear materials. The two agencies agreed to collaborate on the project in response to inconsistencies that were found in the various versions of software and hardware used to determine the isotopic abundances of uranium and plutonium. IRSN used software developed internally to test the MGA and FRAM isotopic analysis codes for criteria used to stop data acquisition. The stop-criterion test revealed several unusual behaviors in both the MGA and FRAM software codes.

2009-01-01

129

The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator

Short communication. 2 figs.

1996-12-31

130

Temporal Fluctuations of Fluorescence Energy Transfer between TwoDyes Conjugated to a Single Protein

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant...Full Text Available

2005-01-01

131

Structure and Function of NtCDPK1, a Calcium-dependent Protein Kinase in Tobccco

No abstract prepared.

1999-01-04

132

Protein profile changes in platelet concentrates according to storage and leukoreduction- analysis using proteomics technology

Full Text Available

2000-05-01

133

Moessbauer studies of the molybdenum-iron-protein in the nitrogenase of Xanthobacter autotrophicus GZ 29. Moessbaueruntersuchungen am Molybdaen-Eisen-Protein der Nitrogenase von Xanthobacter Autotrophicus GZ 29

Full Text Available

2008-02-01

134

METHOD FOR SYNTHESIZING TAURINE FROM PROTEIN-CONTAINING MATERIAL BY USING HIGH-TEMPERATURE HIGH-PRESSURE WATER

Biological cells of the nitrogen fixating bacteria Xanthobacter autotrophicus GZ 29 have been cultivated with 57-Fe, and from the nitrogenase of these cells the 57-Fe marked molybdenum-iron-protein is extracted. The Moessbauer spectra of this enzyme show that the structure of the iron cluster is similar to the structure in the molybdenum-iron-proteins of Azotobacter vinelandii, Klebsiella pneumoniae or Clostridium pasteurianum.

1983-05-01

135

J-STORE (Japan)

Full Text Available

2003-08-28

136

High Throughput Screening on Angiogenesis Inhibitor and Promoter of Medicinal Plants using a Protein Microarray Chip

Expression of microtubule associated protein of low molecular weight (TAU) in mouse brain development

Full Text Available

2007-04-01

137

Effect of the Toluene Administration on the Liver Damage in Rats Fed Low or Standard Protein Diet

Published in summary form only.

138

Effect of nitric oxide on endoplasmic reticulum calcium homeostasis, protein synthesis and energy metabolism

Full Text Available

1996-12-01

139

Effect of Dietary Protein on the Changes of Lipoprotein Fractions in Carbon Tetrachloride-Treated Rats

ATPase, endoplasmic reticulum, calcium, ischaemia

2000-01-01

140

Effect of Dietary Energy, Protein on Growth and Blood Composition of Cross Bred Chicks

Full Text Available

1993-04-01

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141

Effect of Angiotensin-I Converting Enzyme Inhibitory from Hydrolysate of Soybean Protein Isolate

Full Text Available

2008-09-01

142

Dyeability and Functionality of Pine Needles Extract (Part II) -Dyeing Properties of Protein Fiber-

Full Text Available

2010-01-01

143

Deposition of Matrix and Crystalloid Storage Proteins during Protein Body Development in the Endosperm of Ricinus communis L. cv. Hale Seeds ¹

Full Text Available

2011-04-01

144

Comparative Study on Protein and Amino Acid Composition of Wild and Cultured Eel

Protein bodies within the endosperm of castor bean (Ricinus communis L. cv. Hale) seeds arise from numerous small vacuoles which progressively become filled with storage protein, of...Full Text Available

1982-06-01

145

Biochemical characterization of Alanine racemase- a spore protein produced by Bacillus anthracis

Full Text Available

1986-01-01

146

Bilateral retinal dysplasia and secondary glaucoma associated with homozygous protein C deficiency.

Full Text Available

2009-01-01

147

Effect of host nutrition on immunity and local immune response of rabbits to Obeliscoides cuniculi

Full Text Available

2005-06-01

148

Bacteriophage P22 capsid size determination: Roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus

In a series of experiments carried out on young and adult rabbits the effect of isocaloric low protein diets containing 4% or 8% protein compared with a diet containing 21% protein on Obeliscoides cuniculi infection was studied. The pathogenesis, resistance and local immunity were assessed after single infections with 10,000 larvae or reinfection with 5000 larvae. Live weight gain was reduced in young and adult rabbits fed the low protein diets, but the establishment of parasites was not substantially influenced by protein deprivation. However, development of worms in the histotrophic phase and parasite fecundity were impaired in association with the low protein diet. Moreover, mild anaemia as well as changes in the mucosal immune response as a result of infection were related to the level of dietary protein. (author). 30 refs, 6 figs, 5 ...

1987-05-11

149

British Library Electronic Table of Contents (United Kingdom)

Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype of F170A and F170K coat protein mutants were isolated. Suppressors were repeatedly found in the coat protein telokin-like domain at position 285, which caused coat protein to assemble into petite procapsids and capsids. Petite capsid assembly strongly correlated to the side chain volume of the substituted amino acid. We hypothesize that larger side chains at position 285 torque the telokin-like domain, changing flexibility of the subunit and intercapsomer contacts. Thus, a sing...

2011-01-01

150

Identification of proteins important for tetracycline (TC) binding to ribosomes by single protein omission reconstitution (SPORE) experiments

Protein expression in white spot syndrome virus infected Penaeus monodon fabricius.

TC inhibits protein synthesis in E. coli by interfering with aminoacyl-tRNA binding to the ribosomal A site, and there is strong evidence that such inhibition results from TC binding to a high affinity site on the 30S subunit. The SPORE approach has been used to define those 30S proteins that are potentially important for high affinity TC binding, measured as the (/sup 3/H)-TC co-sedimenting with the reconstitution particle through a sucrose density gradient. Reverse phase-HPLC has been used both to prepare ribosomal proteins and to analyze the protein content of reconstituted particles. The results obtained so far show that protein S7, as well as some proteins linked to S7 in the 30S assembly map, are important for TC binding, whereas other ribosomal proteins are not. These results are in very good accord with their earlier photoaffinity ...

1987-05-01

151

T-2 mycotoxin inhibits mitochondrial protein synthesis

White spot syndrome virus (WSSV) is the causative agent of the white spot disease of shrimp. Penaeus monodon were captured from Muttukadu Estuary in Chennai, India, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. WSSV-free P. monodon were challenged by feeding them only once with WSSV-infected tissues of P. monodon. Cumulative mortality (100%) of the infected individuals was determined. Tissues from infected and uninfected shrimp such as muscles, hepatopancreas, heart, gills and eye tissues (100mg of each) and haemolymph (50 microl) were subjected to SDS-PAGE. In infected muscle tissue, six newly expressed proteins were detected. In infected haemolymph, four new proteins and three intensely expressed high molecular weight proteins were observed. Three intensely expressed high molecular weight proteins were detected in infected heart ...

2005-06-01

152

Phosphorylation of proteins in Clostridium thermohydrosulfuricum

The authors investigated the effect of T-2 toxin on rat liver mitochondrial protein synthesis. Isolated rat liver mitochondria were supplemented with an S-100 supernatant from rat liver and an external ATP-generating system. An in-vitro assay employing cycloheximide, and inhibitor of cytoplasmic protein synthesis, and chloramphenicol, and inhibitor of mitochondrial protein synthesis, to distinguish mitochondrial protein synthesis from the cytoplasmic process. Amino acid incorporation into mitochondria was dependent on the concentration of mitochondria and was inhibited by chloramphenicol. The rate of uptake of tritium leucine into mitochondrial protein was unaffected by the addition of T-2 toxin and was not a rate-limiting step in incorporation. However, 0.02 micrograms/ml of T-2 toxin decreased the rate of protein synthesis inhibition correlated with the amount ...

1988-01-01

153

Affinity purification of sequence-specific DNA binding proteins.

Cell extracts of the thermophile Clostridium thermohydrosulfuricum catalyzed the phosphorylation by (..gamma..-/sup 32/P)ATP of several endogenous proteins with M/sub r/s between 13,000 and 100,000. Serine and tyrosine were the main acceptors. Distinct substrate proteins were found in the soluble (e.g., proteins p66, p63, and p53 of M/sub r/s 66,000, 63,000, and 53,000, respectively) and particulate (p76 and p30) fractions, both of which contained protein kinase and phosphatase activity. The soluble fraction suppressed the phosphorylation of particulate proteins and contained a protein kinase inhibitor. Phosphorylation of p53 was promoted by 10..mu..M fructose 1,6-bisphosphate or glucose 1,6-bisphosphate and suppressed by hexose monophosphates, whereas p30 and p13 were suppressed by 5 ..mu..M brain (but not spinach) calmodulin. Polyamines, including the odd ...

1986-02-01

154

The Sorcerer II Global Ocean Sampling Expedition: Expanding theUniverse of Protein Families

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This DNA is then covalently coupled to Sepharose CL-2B with cyanogen bromide to yield the affinity resin. A partially purified protein fraction is combined with competitor DNA and subsequently passed through the DNA-Sepharose resin. The desired sequence-specific DNA binding protein is purified because it preferentially binds to the recognition sites in the affinity resin rather than to the nonspecific competitor DNA in solution. For example, a protein fraction that is enriched for transcription factor Sp1 can be further purified 500- to 1000-fold by two sequential affinity chromatography ...

1986-08-01

155

Interactive efforts to address DSM and IRP issues: Findings from the first year of a two-year study

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several ...

2006-03-23

156

Interactive efforts to address DSM and IRP issues: Findings from the first year of a two-year study

This report presents findings from the first year of a two-year study of interactive efforts involving utilities and non-utility parties (NUPS) working together to prepare plans, develop Demand-Side Management (DSM) programs, or otherwise promote integrated planning and the use of cost-effective DSM measures. Of the ten cases covered in the current study, seven involved the collaborative approach to NUP involvement, which generally is marked by intensive utility-NUP interactions designed to reach consensus on a broad range of important issues; in collaboratives, outside consultants often are provided to enhance the technical capabilities of the NUPS. Another of the cases in this study involved a cooperative arrangement,'' whereby a utility and a NLT worked together in a focused short-term effort to develop a single DSM program. The intense interaction involved in this approach makes it very similar to a ...

1993-04-01

157

Uniform and Residue-specific {sup 15}N-labeling of Proteins on a Highly Deuterated Background

This report presents findings from the first year of a two-year study of interactive efforts involving utilities and non-utility parties (NUPS) working together to prepare plans, develop Demand-Side Management (DSM) programs, or otherwise promote integrated planning and the use of cost-effective DSM measures. Of the ten cases covered in the current study, seven involved the collaborative approach to NUP involvement, which generally is marked by intensive utility-NUP interactions designed to reach consensus on a broad range of important issues; in collaboratives, outside consultants often are provided to enhance the technical capabilities of the NUPS. Another of the cases in this study involved a ``cooperative arrangement,`` whereby a utility and a NLT worked together in a focused short-term effort to develop a single DSM program. The intense interaction involved in this approach makes it very similar to a collaborative, ...

1993-04-01

158

Proteomic analysis of sericin in Bombyx mori cocoons

A general method for stable-isotope labeling of large proteins is introduced and applied for studies of the E. coli GroE chaperone proteins by solution NMR. In addition to enabling the residue-specific {sup 15}N-labeling of proteins on a highly deuterated background, it is also an efficient approach for uniform labeling. The method meets the requirements of high-level deuteration, minimal cross-labeling and high protein yield, which are crucial for NMR studies of structures with sizes above 150 kDa. The results obtained with the new protocol are compared to other strategies for protein labeling, and evaluated with regard to the influence of external factors on the resulting isotope labeling patterns. Applications with the GroE system show that these strategies are efficient tools for studies of structure, dynamics and intermolecular interactions in large supramolecular complexes, ...

2004-07-15

159

British Library Electronic Table of Contents (United Kingdom)

Cocoon sericin plays an important role in the reeling of silk and serves as a valuable biomaterial in the field of biomedicine, skincare, and food industries; however, knowledge about cocoon sericin proteins has been limited. For a comprehensive study on sericin, cocoons of eight varieties of silkworm of different geographic origin and with varied cocoon color were analyzed utilizing proteomics and bioinformatics approaches. The electrophoresis pattern demonstrated some common protein bands for all silkworm varieties and distinctive protein bands for some of those examined in the present study. The Ser2 protein, a new Ser3 protein, and four other novel sericin proteins were identified in cocoons for the first time. Products of both Ser1 and Ser3 genes appear to be ubiquitous in the cocoon ...

2011-01-01

160

Modeling protein synthesis from a physicist's perspective: a toy model

Mechanism of Dephosphorylation of the SR Protein ASF/SF2 by Protein Phosphatase 1

Proteins are polymers of amino acids. These macromolecules are synthesized by intracellular machines called {\\it ribosome}. Although, traditionally, the experimental investigation of protein synthesis has been an active area of research in molecular cell biology, important quantitative models of this phenomenon have been reported mostly in the research journals devoted to statistical physics and related interdisciplinary topics. From the perspective of a physicist, protein synthesis is a phenomenon of {\\it classical transport of interacting ribosomes on a messenger RNA (mRNA) template} that dictates the sequence of the amino acids on the protein. Here we bring this frontier area of contemporary research into the classroom by appropriate simplification of the models and methods. In particular, we develope a simple toy model and analyze it by some elementary techniques of non-equilibrium statistical ...

2007-01-01

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161

British Library Electronic Table of Contents (United Kingdom)

SR proteins are essential splicing factors whose function is controlled by multi-site phosphorylation of a C-terminal domain rich in arginine-serine repeats (RS domain). The protein kinase SRPK1 has been shown to polyphosphorylate the N-terminal portion of the RS domain (RS1) of the SR protein ASF/SF2, a modification that promotes nuclear entry of this splicing factor and engagement in splicing function. Later, dephosphorylation is required for maturation of the spliceosome and other RNA processing steps. While phosphates are attached to RS1 in a sequential manner by SRPK1, little is known about how they are removed. To investigate factors that control dephosphorylation, we monitored region-specific mapping of phosphorylation sites in ASF/SF2 as a function of the protein phosphatase PP1. W...

2010-01-01

162

Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8

British Library Electronic Table of Contents (United Kingdom)

This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50??g/mL) reduced the population of B. cereus from >108 to 104 colony-forming units per milliliter within 30?min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.

2011-01-01

163

Review of Experimental Studies of $\\psi(3770)$ non-$D\\bar D$ Decays

Fluxnet Synthesis Dataset Collaboration Infrastructure

We review the progress on experimental studies of the non-$D\\bar D$ decays of the $\\psi(3770)$ resonance. With the world average of the observed cross sections for $D\\bar D$ production measured at 3.773 GeV by the MARK-I, MARK-II, BES and CLEO Collaborations, combined together with the cross section for $\\psi(3770)$ production at its peak as well as initial state radiative correction factor, we find that the non-$D\\bar D$ branching fraction of $\\psi(3770)$ decays is $B[\\psi(3770)\\to {\\rm non}-D\\bar D]=(19.8\\pm 1.8 \\pm 5.6)%$, which is consistent within error with $B[\\psi(3770)\\to {\\rm non}-D\\bar D]=(14.7\\pm 3.2)%$ measured previously by the BES Collaboration. In addition, a global amplitude analysis of the cross sections for $e^+e^- \\to {\\rm LH}$ (LH= light hadron) measured by the CLEO Collaboration shows that the light hadron branching fraction of $\\psi(3770)$ decays can be as large as about 11%. ...

2010-01-01

164

The Fluxnet synthesis dataset originally compiled for the La Thuile workshop contained approximately 600 site years. Since the workshop, several additional site years have been added and the dataset now contains over 920 site years from over 240 sites. A data refresh update is expected to increase those numbers in the next few months. The ancillary data describing the sites continues to evolve as well. There are on the order of 120 site contacts and 60proposals have been approved to use thedata. These proposals involve around 120 researchers. The size and complexity of the dataset and collaboration has led to a new approach to providing access to the data and collaboration support and the support team attended the workshop and worked closely with the attendees and the Fluxnet project office to define the requirements for the support infrastructure. As a result of this effort, a new website (http://www.fluxdata.org) has been created to provide ...

2008-02-06

165

Annual report 2005-2006

Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis

Research and development and other activities of the various constituent units of Department of Atomic Energy (DAE) and also of the institution aided by DAE for the year 2005-2006 are reported. The various constituents units of DAE consist of nuclear research centres, nuclear power stations, fuel reprocessing and heavy water plants, nuclear fuel fabrication facilities, electronic and instrumentation production organisations, atomic mineral processing units and other nuclear installations. The activities of DAE cover the whole gamut of nuclear fuel cycle, research and development in nuclear science and reactor technology, applications of radiation and radioisotopes, radiation protection, research and development in front line areas such as robotics, lasers, mathematics and computational sciences. International research collaborations like CERN-DAE collaboration were completed by BARC. These activities are described broadly under: executive ...

166

Werner syndrome protein interacts functionally with translesion DNA polymerases

Wiskott-Aldrich syndrome protein (WASp) is essential for optimal T cell activation. Patients with WAS exhibit both immunodeficiency and a marked susceptibility to systemic autoimmunity. We investigated...Full Text Available

2007-02-01

167

Water Molecule Contributions to Proton Spin-Lattice Relaxation in Rotationally Immobilized Proteins

Werner syndrome (WS) is characterized by premature onset of age-associated disorders and predisposition to cancer. The WS protein, WRN, encodes 3′ → 5′ DNA helicase and 3′...Full Text Available

2007-06-19

168

Variable domain I of nematode CLEs directs post-translational targeting of CLE peptides to the extracellular space

Spin-lattice relaxation rates of protein and water protons in dry and hydrated immobilized bovine serum albumin were measured in the range of ¹H Larmor frequency from 10 kHz to 30...Full Text Available

2009-07-01

169

Use of synthetic oligoribonucleotides to probe RNA-protein interactions in the MS2 translational operator complex.

Effector proteins expressed in the esophageal gland cells of cyst nematodes are delivered into plant cells through a hollow, protrusible stylet. Although evidence indicates that effector proteins function...Full Text Available

2010-12-01

170

Use of Dimethyl Suberimidate, a Cross-Linking Reagent, in Studying the Subunit Structure of Oligomeric Proteins^*

Synthetic oligoribonucleotides have been used to probe the interaction of MS2 coat protein with the translational operator of the MS2 replicase gene. We have investigated the possible formation of a...Full Text Available

1990-06-25

171

Use of DNA ladders for reproducible protein fractionation by SDS-PAGE for quantitative proteomics

Amidination of aldolase, glyceraldehyde-3-phosphate dehydrogenase, tryptophan synthetase B protein, L-arabinose isomerase, and the catalytic subunit of E. coli aspartate transcarbamylase...Full Text Available

1970-07-01

172

The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription

In proteomics, one-dimensional (1D) SDS-PAGE is widely used for protein fractionation prior to mass spectrometric analysis to enhance dynamic range of analysis and to improve identification...Full Text Available

2008-02-01

173

The region encompassing the procyclic acidic repetitive protein (PARP) gene promoter plays a role in plasmid DNA replication in Trypanosoma brucei.

The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex...Full Text Available

2005-01-01

174

The long lifespan of two bat species is correlated with resistance to protein oxidation and enhanced protein homeostasis

We have previously reported the construction and characterization of an autonomously replicating plasmid in Trypanosoma brucei. In this plasmid the procyclic acidic repetitive protein (PARP) gene promoter...Full Text Available

1994-10-11

175

The discovery of signal transduction by G proteins. A personal account and an overview of the initial findings and contributions that led to our present understanding

Altered structure, and hence function, of cellular macromolecules caused by oxidation can contribute to loss of physiological function with age. Here, we tested whether the lifespan of bats, which generally...Full Text Available

2009-07-01

176

The Effects of Aspirin-Like Drugs on the Nutritional Status of Pregnant Rats and Offspring

The realization that there existed a G-protein coupled signal transduction mechanism developed gradually and was initially the result of an ill fated quest for uncovering the mechanism of action...Full Text Available

2007-04-01

177

Synthesis and Phosphorylation of Maize Acidic Ribosomal Proteins1

Previous studies have shown that salicylates and protein-calorie malnutrition independently compromise maturation and growth of infants. In the present study, pregnant rats were fed normal-and low-protein...Full Text Available

1988-05-01

178

Surfactant protein A, an innate immune factor, is expressed in the vaginal mucosa and is present in vaginal lavage fluid

The objective of this research was to determine the role of acidic ribosomal protein (ARP) phosphorylation in translation. Ribosomes (Rbs) from germinated maize (Zea mays L.) axes had...Full Text Available

1998-01-01

179

Stimulation of Phosphatidylethanolamine Exchange by Castor Bean Cytosol Proteins ¹

Surfactant protein A (SP-A), first identified as a component of the lung surfactant system, is now recognized to be an important contributor to host defence mechanisms. SP-A can facilitate phagocytosis...Full Text Available

2004-01-01

180

Simulation of the electron-paramagnetic-resonance spectrum of the iron-protein of nitrogenase. A prediction of the existence of a second paramagnetic centre.

Cytosol proteins prepared from castor bean endosperm (4-day-old) seedlings stimulate the exchange of [³H]phosphatidylethanolamine between liposomes and mitochondria. The acceleration of the...Full Text Available

1980-02-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

181

Seroprevalence of HIV-1 and HIV-2 infection among children diagnosed with protein-calorie malnutrition in Nigeria.

The e.p.r. spectra of the Fe-proteins of nitrogenase from all sources studied have unusual features in that they have very anisotropic linewidths and low integrated intensities. These characteristics...Full Text Available

1978-12-01

182

Role in Virulence of a Brucella abortus Protein Exhibiting Lectin-Like Activity

Excessive weight loss due to protein calorie malnutrition (PCM) is a significant problem in Nigerian children. This syndrome may be difficult to differentiate from the wasting disease caused by human...Full Text Available

1993-04-01

183

Risk factors for thrombosis in lupus patients.

Brucella abortus is a facultative, intracellular zoonotic pathogen which can cause undulant fever in humans and abortions in cattle. A 14-kDa protein of B. abortus...Full Text Available

2006-01-01

184

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

Lupus anticoagulant, concentrations of anticardiolipin antibodies, antithrombin III, plasminogen, (free) protein S, protein C, prothrombin, platelet counts, and bleeding times were determined in 74...Full Text Available

1989-11-01

185

Proteomic approach with LCMS-IT-TOF identified an increase of Rab33B after transient focal cerebral ischemia in mice

Carbenoxolone (CBX) is a semisynthetic derivative of the licorice root substance glycyrrhizinic acid and has been previously reported to induce only heat shock protein 70 [Hsp70, HSPA1A (the systematic...Full Text Available

2009-09-01

186

Predicting Protein Post-translational Modifications Using Meta-analysis of Proteome Scale Data Sets*S?

BackgroundSeveral proteins are known to be markedly expressed in the brain during cerebral ischemia; however, the changes in protein profiles within the ischemic brain after an ischemic...Full Text Available

187

Plasma protein binding of phenytoin in 100 epileptic patients.

Protein post-translational modifications are an important biological regulatory mechanism, and the rate of their discovery using high throughput techniques is rapidly increasingly. To make use of this...Full Text Available

2009-02-01

188

Photophysics and optical switching in green fluorescent protein mutants

The plasma protein binding of phenytoin was investigated in 100 epileptic patients, using equilibrium dialysis at 37 degrees C. The unbound fractions of phenytoin in plasma formed a skewed distribution,...Full Text Available

1982-08-01

189

Pharmacokinetics and protein binding of ceftriaxone during pregnancy.

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not...Full Text Available

2000-03-28

190

Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

The purpose of the present work was to study the pharmacokinetics and the protein binding (free fraction of the drug) of ceftriaxone (CTX) during pregnancy. Nine pregnant women (ages, 20 to 34 years)...Full Text Available

1993-01-01

191

Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains

BackgroundThe expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations...Full Text Available

192

Multiple-Bond Kinetics from Single-Molecule Pulling Experiments: Evidence for Multiple NCAM Bonds

BackgroundZhx1 to 3 (zinc-fingers and homeoboxes) form a set of paralogous genes encoding multi-domain proteins. ZHX proteins consist of two zinc fingers followed...Full Text Available

193

Molecular Identification and Expression Analysis of Filaggrin-2, a Member of the S100 Fused-Type Protein Family

The kinetic parameters of single bonds between neural cell adhesion molecules were determined from atomic force microscope measurements of the forced dissociation of the homophilic protein-protein bonds....Full Text Available

2005-11-01

194

Molecular Architecture of the Centriole Proteome: The Conserved WD40 Domain Protein POC1 Is Required for Centriole Duplication and Length Control

Genes of the S100 fused-type protein (SFTP) family are clustered within the epidermal differentiation complex and encode essential components that maintain epithelial homeostasis and barrier functions....Full Text Available

195

Metal Ions-Stimulated Iron Oxidation in Hydroxylases Facilitates Stabilization of HIF-1? Protein

Centrioles are intriguing cylindrical organelles composed of triplet microtubules. Proteomic data suggest that a large number of proteins besides tubulin are necessary for the formation and maintenance...Full Text Available

2009-02-15

196

Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

The exposure of cells to several metal ions stabilizes HIF-1α protein. However, the molecular mechanisms are not completely understood. They may involve inhibition of hydroxylation by either...Full Text Available

2009-02-01

197

Measurements of accurate x-ray scattering data of protein solutions using small stationary sample cells

Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing...Full Text Available

2010-01-01

198

MFR, a Putative Receptor Mediating the Fusion of Macrophages

In this paper, we report a method of precise in situ x-ray scattering measurements on protein solutions using small stationary sample cells. Although reduction in the radiation damage...Full Text Available

2009-01-01

199

M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.

We had previously identified a macrophage surface protein whose expression is highly induced, transient, and specific, as it is restricted to actively fusing macrophages in vitro and in vivo. This protein...Full Text Available

1998-11-01

200

Los Alamos sequence analysis package for nucleic acids and proteins.

A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three...Full Text Available

1989-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

201

Lipoprotein lipase deficiency is associated with elevated acylation stimulating protein plasma levels

An interactive system for computer analysis of nucleic acid and protein sequences has been developed for the Los Alamos DNA Sequence Database. It provides a convenient way to search or verify various...Full Text Available

1982-01-11

202

Linking Gene Expression and Functional Network Data in Human Heart Failure

Acylation stimulating protein (ASP, C3adesArg) is an adipose tissue derived hormone that stimulates triglyceride (TG) synthesis. ASP stimulates lipoprotein lipase (LPL) activity by relieving feedback...Full Text Available

2009-06-01

203

Lifespan extension by increased expression of the Drosophila homologue of the IGFBP7 tumour suppressor

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

204

Lectin Binding of the Major Polar Tube Protein (PTP1) and its Role in Invasion

Mammals possess multiple insulin-like growth factor (IGF) binding proteins (IGFBPs), and related proteins, that modulate the activity of insulin/IGF signalling (IIS), a conserved neuroendocrine signalling...Full Text Available

2011-02-01

205

Large-scale Analysis of Thermo-stable, Mammalian Proteins Provides Insights into the Intrinsically Disordered Proteome

... 2003)050[0600:LBOTMP]2.0.CO;2 Lectin Binding of the Major Polar Tube Protein (PTP1) ... PTP1. We, therefore, undertook a study ...

206

Lack of association between polymorphisms in C4b-binding protein and atypical haemolytic uraemic syndrome in the Spanish population

Intrinsically disordered proteins are predicted to be highly abundant and play broad biological roles in eukaryotic cells. In particular, by virtue of their structural malleability and propensity...Full Text Available

2009-01-01

207

Isolation and Characterization of a Novel Nuclear Protein from Pollen Mother Cells of Lily

Dysregulation of the alternative pathway of complement activation, caused by mutations or polymorphisms in the genes encoding factor H, membrane co-factor protein, factor I or factor B, is associated...Full Text Available

2009-01-01

208

Involvement of AMP-activated-protein-kinase (AMPK) in neuronal amyloidogenesis

Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G₂...Full Text Available

1990-11-01

209

Investigation of Early Protein Changes in the Urinary Bladder Following Partial Bladder Outlet Obstruction by Proteomic Approach

AMP-activated-protein-kinase (AMPK) is a key sensor and regulator of cellular and whole-body energy metabolism and plays a key role in regulation of lipid metabolism. Since lipid metabolism...Full Text Available

2010-09-03

210

Intracellular delivery of an antisense?oligonucleotide via endocytosis of a G protein-coupled receptor

We investigated the pathophysiological mechanism by proteomic approach as a possible tool to detect the marker proteins to develop lower urinary tract symptoms following bladder outlet obstruction (BOO)....Full Text Available

2005-12-01

211

Interaction between Bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egress

Gastrin-releasing peptide receptor (GRPR), a member of the G protein-coupled receptor superfamily, has been utilized for receptor-mediated targeting of imaging and therapeutic agents; here we extend...Full Text Available

2010-10-01

212

Insulin-induced decrease in protein phosphorylation in rat adipocytes not explained by decreased A-kinase activity

BackgroundThe VP2 outer capsid protein Bluetongue Virus (BTV) is responsible for receptor binding, haemagglutination and eliciting host-specific immunity. However, the assembly of...Full Text Available

213

Insertion in the mRNA of a metachromatic leukodystrophy patient with sphingolipid activator protein-1 deficiency.

In isolated rat adipocytes, insulin inhibits lipolysis to a greater extent than would be predicted by the decrease in (-/+)cAMP activity ratio of cAMP-dependent protein kinase (A-kinase), from which it was speculated that insulin promotes the dephosphorylation of hormone-sensitive lipase. They have examined the phosphorylation state of cellular proteins under conditions of varying A-kinase activities in the presence and absence of insulin. Protein phosphorylation was determined by SDS-PAGE electrophoresis of extracts from /sup 32/P-loaded cells; glycerol and A-kinase activity ratios were measured in the cytosolic extracts from control, non-radioactive cells. Increased protein phosphorylation in general occurred over the same range of A-kinase activity ratios, 0.1-0.3, associated with increased glycerol release. The insulin-induced decrease in lipolysis was associated with a decrease in the /sup 32/P ...

1987-05-01

214

Inferring Stabilizing Mutations from Protein Phylogenies: Application to Influenza Hemagglutinin

The lysosomal catabolism of sulfatide requires arylsulfatase A and a specific sphingolipid activator protein, SAP-1. While most patients with metachromatic leukodystrophy have mutations in the gene...Full Text Available

1990-02-01

215

Individual Mycobacterium tuberculosis universal stress protein homologues are dispensable in vitro

One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions. We present...Full Text Available

2009-04-01

216

In vitro protein binding of cefonicid and cefuroxime in adult and neonatal sera.

SummaryMycobacterium tuberculosis has 10 universal stress proteins, whose function is unknown. However, proteomic and transcriptomic analyses have shown that a number...Full Text Available

2010-07-01

217

Identification of Host Proteins Associated with Retroviral Vector Particles by Proteomic Analysis of Highly Purified Vector Preparations?

The levels of in vitro protein binding of cefonicid and cefuroxime in human adult and neonatal sera were compared. Binding parameters for each drug were determined within the concentration range of...Full Text Available

1993-06-01

218

How Protein Stability and New Functions Trade Off

The Moloney murine leukemia virus (MMLV) belongs to the Retroviridae family of enveloped viruses, which is known to acquire minute amounts of host cellular proteins both on the surface...Full Text Available

2008-02-01

219

Functional properties of membrane cofactor protein of complement.

Numerous studies have noted that the evolution of new enzymatic specificities is accompanied by loss of the protein's thermodynamic stability (ΔΔG), thus suggesting...Full Text Available

2008-02-01

220

Fatty Acid- and Retinoid-binding Proteins Have Distinct Binding Pockets for the Two Types of Cargo*

Membrane cofactor protein (MCP or gp45-70) of the complement system is a cofactor for factor I-mediated cleavage of fluid-phase C3b and C3b-like C3, which opens the thioester bond. In the present study...Full Text Available

1989-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

221

Evidence for nonrandom hydrophobicity structures in protein chains.

Parasitic nematodes cause serious diseases in humans, animals, and plants. They have limited lipid metabolism and are reliant on lipid-binding proteins to acquire these metabolites from their hosts....Full Text Available

2009-12-18

222

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay

The question of whether proteins originate from random sequences of amino acids is addressed. A statistical analysis is performed in terms of blocked and random walk values formed by binary hydrophobic...Full Text Available

1996-09-03

223

Evaluation of Two Homologous Proline-Rich Proteins of Coccidioides posadasii as Candidate Vaccines against Coccidioidomycosis?

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis ...

1994-08-01

224

EhMAPK, the Mitogen-Activated Protein Kinase from Entamoeba histolytica Is Associated with Cell Survival

Evaluation of the protective efficacy of recombinant T-cell-reactive proteins of Coccidioides posadasii in a murine model of coccidioidomycosis has led to the discovery of potential...Full Text Available

2007-12-01

225

Effects of PKA phosphorylation on the conformation of the Na,K-ATPase regulatory protein FXYD1

Mitogen Activated Protein Kinases (MAPKs) are a class of serine/threonine kinases that regulate a number of different cellular activities including cell proliferation, differentiation, survival and...Full Text Available

226

Effects of Essential Oils on Ruminal Microorganisms and Their Protein Metabolism

FXYD1 (phospholemman) is a member of an evolutionarily conserved family of membrane proteins that regulate the function of the Na,K-ATPase enzyme complex in specific tissues and specific physiological...Full Text Available

2009-11-01

227

E2f binding-deficient Rb1 protein suppresses prostate tumor progression in vivo

A commercial blend of essential oil (EO) compounds was added to a grass, maize silage, and concentrate diet fed to dairy cattle in order to determine their influence on protein metabolism by ruminal...Full Text Available

2003-08-01

228

Display of Recombinant Proteins on Bacillus subtilis Spores, Using a Coat-Associated Enzyme as the Carrier?

Mutational inactivation of the RB1 tumor suppressor gene initiates retinoblastoma and other human cancers. RB1 protein (pRb) restrains cell proliferation by binding...Full Text Available

2011-01-11

229

Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice

The display of proteins such as feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater...Full Text Available

2010-09-01

230

Determinants of the plasma protein binding of theophylline in health.

Uncoupling protein 2 (UCP2) maps to a region on distal mouse chromosome 7 that has been linked to the phenotypes of obesity and type II diabetes. We recently reported that UCP2 expression is increased...Full Text Available

1998-03-31

231

Dengue virus protein recognition by virus-specific murine CD8+ cytotoxic T lymphocytes.

1 The plasma protein binding of theophylline was determined after addition of [14C]-theophylline (15 micrograms/ml) to plasma from 24 healthy drug-free volunteers and equilibrium dialysis for 2 h at...Full Text Available

1983-04-01

232

Deletion of the Plasmodium falciparum Merozoite Surface Protein 7 Gene Impairs Parasite Invasion of Erythrocytes?

The identification of the protein targets for dengue virus-specific T lymphocytes may be useful for planning the development of subunit vaccines against dengue. We studied the recognition by murine...Full Text Available

1993-02-01

233

Degradation of Proteins Artificially Introduced into Vacuoles of Chara australis¹

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria...Full Text Available

2008-12-01

234

Deglutathionylation of 2-Cys Peroxiredoxin Is Specifically Catalyzed by Sulfiredoxin*

When an exogenous protein, bovine serum albumin, was introduced into the vacuole of a Chara australis internodal cell, it was degraded with time. This degradation proceeded only in...Full Text Available

1988-12-01

235

Deficiency of circadian protein CLOCK reduces lifespan and increases age-related cataract development in mice

Reversible protein glutathionylation plays a key role in cellular regulation and cell signaling and protects protein thiols from hyperoxidation. Sulfiredoxin (Srx), an enzyme that catalyzes the reduction...Full Text Available

2009-08-28

236

DNA replication in Escherichia coli mutants that lack protein HU.

Circadian clock is implicated in the regulation of aging. The transcription factor CLOCK, a core component of the circadian system, operates in complex with another circadian clock protein BMAL1. Recently...Full Text Available

237

Cyclic Diguanylate Signaling Proteins Control Intracellular Growth of Legionella pneumophila

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

1989-10-01

238

Coupling of Pressure-Induced Structural Shifts to Spectral Changes in a Yellow Fluorescent Protein

Proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. These processes include motility, biofilm formation, cell...Full Text Available

239

Controlling Gas-Phase Reactions for Efficient Charge Reduction Electrospray Mass Spectrometry of Intact Proteins

AbstractX-ray diffraction analysis of pressure-induced structural changes in the Aequorea yellow fluorescent protein Citrine reveals the structural basis for the continuous...Full Text Available

2009-09-16

240

Computer-based analysis of Haemophilus parasuis protein fingerprints

Charge reduction electrospray mass spectrometry (CREMS) reduces the charge states of electrospray-generated ions, which concentrates the ions from a protein into fewer peaks spread over a larger...Full Text Available

2005-11-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Computer simulation of protein self-association during small-zone gel filtration. Estimation of equilibrium constants.

AbstractThe present study aimed to compare the whole-cell protein profiles of Haemophilus parasuis field isolates by using a computer-based analysis, and evaluate the...Full Text Available

2004-01-01

242

Complement Factor H-Related Proteins CFHR2 and CFHR5 Represent Novel Ligands for the Infection-Associated CRASP Proteins of Borrelia burgdorferi

A simulation is developed that qualitatively describes the small-zone-gel-filtration behaviour of a reversibly associating protein. The results reflect the dependence of the apparent molecular weight...Full Text Available

1981-04-01

243

Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues

BackgroundOne virulence property of Borrelia burgdorferi is its resistance to innate immunity, in particular to complement-mediated killing. Serum-resistant B....Full Text Available

244

Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis.

AIM: To investigate the relation of Fas and Fas ligand (FasL) protein expression with carcinogenesis and metastasis of gastric carcinoma.METHODS: Immunohistochemistry was used to detect Fas and...Full Text Available

2010-03-14

245

Circulating antibody to myelin basic protein in relapsing-remitting multiple sclerosis? A comparative group and sequential study by radioimmunoassay.

Cellular cholesterol homeostasis is controlled by sterol-regulated proteolysis of membrane-bound transcription factors called sterol-regulatory element binding proteins (SREBPs). CPP32, a cysteine protease,...Full Text Available

1996-03-01

246

Characterization of group A streptococcal T-12 protein purified by ion-exchange column chromatography.

Sera from multiple sclerosis patients with relapsing-remitting disease and normal subjects were tested for antibody to myelin basic protein by a sensitive radioimmunoassay. The results showed a marginally...Full Text Available

1978-12-01

247

Characteristics of the molecular diversity of the outer membrane protein A gene of Haemophilus parasuis

The aim of the present study was to describe the physicochemical characteristics of streptococcal T antigen. T protein isolated from Streptococcus pyogenes type 12 (R53/1077, Colindale) and purified...Full Text Available

1978-09-01

248

CID755673 enhances mitogenic signaling by phorbol esters, bombesin and EGF through a protein kinase D-independent pathway

The molecular diversity of the gene encoding the outer membrane protein A (OmpA) of Haemophilus parasuis has been unclear. In this study, the structural characteristics, sequence types,...Full Text Available

2010-07-01

249

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

Recently, CID755673 was reported to act as a highly selective inhibitor of protein kinase D (PKD). In the course of experiments using CID755673, we noticed that it exerted unexpected stimulatory...Full Text Available

2010-01-01

250

Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have...Full Text Available

2010-10-01

251

Association of Protein Phosphatase 1?₁ with Spinophilin Suppresses Phosphatase Activity in a Parkinson Disease Model*

Certain pathogenic species of Bacillus and Clostridium have developed unique methods for intoxicating cells that employ the classic enzymatic “A-B” paradigm for protein toxins. The binary...Full Text Available

2004-09-01

252

Application of the PM6 semi-empirical method to modeling proteins enhances docking accuracy of AutoDock

Sustained nigrostriatal dopamine depletion increases the serine/threonine phosphorylation of multiple striatal proteins that play a role in corticostriatal synaptic plasticity, including Thr²⁸⁶...Full Text Available

2008-05-23

253

Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models

BackgroundMolecular docking methods are commonly used for predicting binding modes and energies of ligands to proteins. For accurate complex geometry and binding energy estimation,...Full Text Available

254

An automated procedure for detecting protein folds from sub-nanometer resolution electron density

PurposeCataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and...Full Text Available

255

Amyloid Oligomer Neurotoxicity, Calcium Dysregulation, and Lipid Rafts

The use of sub-nanometer resolution electron density as spatial constraints for denovo and ab-initio structure prediction requires knowledge of protein boundaries...Full Text Available

2010-06-01

256

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Amyloid proteins constitute a chemically heterogeneous group of proteins, which share some biophysical and biological characteristics, the principal of which are the high propensity to acquire an incorrect...Full Text Available

257

Albumin binding of insulins acylated with fatty acids: characterization of the ligand-protein interaction and correlation between binding affinity and timing of the insulin effect in vivo.

BackgroundThe production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically...Full Text Available

258

Agonist-dependent up-regulation of thyrotrophin-releasing hormone receptor protein.

Albumin is a multifunctional transport protein that binds a wide variety of endogenous substances and drugs. Insulins with affinity for albumin were engineered by acylation of the epsilon-amino group...Full Text Available

1995-12-15

259

Adhesion of DOPA-Functionalized Model Membranes to Hard and Soft Surfaces

To study the effect of agonist on the TRH (thyrotrophin-releasing hormone) receptor protein, an epitope-tagged receptor was stably expressed in HEK-293 cells (human embryonic kidney 293 cells) and receptor...Full Text Available

2004-06-15

260

Absence of Ataxin-3 Leads to Enhanced Stress Response in C. elegans

The adhesive proteins secreted by marine mussels form a natural glue that cures rapidly to form strong and durable bonds in aqueous environments. These mussel adhesive proteins contain an unusual...Full Text Available

2009-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

AMP-activated protein kinase (AMPK) cross-talks with canonical Wnt signaling via phosphorylation of ?-catenin at Ser 552

Ataxin-3, the protein involved in Machado-Joseph disease, is able to bind ubiquitylated substrates and act as a deubiquitylating enzyme in vitro, and it has been involved in the modulation of protein...Full Text Available

262

AMP-Activated Protein Kinase: A Stress-Responsive Kinase with Implications for Cardiovascular Disease

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism; its activity is regulated by a plethora of physiological conditions, exercises and many anti-diabetic drugs. Recent...Full Text Available

2010-04-23

263

AICD Overexpression in Neuro 2A Cells Regulates Expression of PTCH1 and TRPC5

AMP-activated protein kinase (AMPK) was initially viewed as energy sensor and activated by increased intracellular concentrations of AMP following nutrient deprivation. Physiological or pathological...Full Text Available

2010-04-01

264

A study in molecular contingency: glutamine phosphoribosylpyrophosphate amidotransferase is a promiscuous and evolvable phosphoribosylanthranilate isomerase

Amyloid precursor protein (APP), implicated in Alzheimer's disease, is a transmembrane protein of undetermined function. APP is cleaved by gamma-secretase that releases the APP intracellular domain...Full Text Available

265

A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning

SummaryThe prevalence of paralogous enzymes implies that novel catalytic functions can evolve on preexisting protein scaffolds. The weak secondary activities of proteins, which...Full Text Available

2008-03-21

266

A microscale protein NMR sample screening pipeline

The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies...Full Text Available

2009-01-01

267

A global optimization algorithm for protein surface alignment

As part of efforts to develop improved methods for NMR protein sample preparation and structure determination, the Northeast Structural Genomics Consortium (NESG) has implemented an NMR screening pipeline for protein target selection, construct optimization, and buffer optimization, incorporating efficient microscale NMR screening of proteins using a micro-cryoprobe. The process is feasible because the newest generation probe requires only small amounts of protein, typically 30-200 ?g in 8-35 ?l volume. Extensive automation has been made possible by the combination of database tools, mechanization of key process steps, and the use of a micro-cryoprobe that gives excellent data while requiring little optimization and manual setup. In this perspective, we describe the overall process used by the NESG for screening NMR samples as part of a sample optimization process, assessing optimal construct design and ...

2010-01-01

268

A genome-wide study of PDZ-domain interactions in C. elegans reveals a high frequency of non-canonical binding

BackgroundA relevant problem in drug design is the comparison and recognition of protein binding sites. Binding sites recognition is generally based on geometry often combined with...Full Text Available

269

A gene encoding a protein modified by the phytohormone indoleacetic acid

BackgroundProteins may evolve through the recruitment and modification of discrete domains, and in many cases, protein action can be dissected at the domain level. PDZ domains are...Full Text Available

270

?Activation of the mitogen-activated protein kinase pathways by heat shock

We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this...Full Text Available

2002-02-05

271

'Sheltered disruption' of Neurospora crassa MOM22, an essential component of the mitochondrial protein import complex.

In addition to inducing new transcriptional activities that lead within a few hours to the accumulation of heat shock proteins (Hsps), heat shock activates within minutes the major signaling transduction...Full Text Available

2002-04-01

272

[ ${\\alpha}$ ]-Amylase and Protein Tyrosine Phosphatase 1B Inhibitory of Some Vietnamese Medicinal Plants Used to Treat Diabetes

MOM22 is a component of the protein import complex of the mitochondrial outer membrane of Neurospora crassa. Using the newly developed procedure of 'sheltered disruption', we created a heterokaryotic...Full Text Available

1995-03-15

273

Uptake of Heavy Metal Ions by Water Dropwort (Oenanthe stolonifera DC.) and Identification of Its Heavy Metal-Binding Protein

Full Text Available

2007-12-01

274

The protein truncation caused by fusion of PEP-1 peptide and protective roles of transduced PEP-1-MsrA in skin cells

Full Text Available

1996-12-01

275

Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae

Full Text Available

2011-04-01

276

Photolabelling reveals differences between cholera toxin and ricin toxin insertion mechanisms

Full Text Available

2009-10-01

277

Liquid nitrogen dewar for protein crystal growth

... carbon 14 cholera gels labelling membranes molecules proteins receptors

1982-07-28

278

Letter to the Editor: 1H, 13C and 15N chemical shift assignments of the capsid protein from Rous sarcoma virus

Gaseous Nitrogen Dewar apparatus developed by Dr. Alex McPherson of the University of California,

2001-01-01

279

Interaction Of Human La Protein With The Internal Ribosome Entry Site Of Hepatitis C Virus : Functional Role In Mediating Internal Initiation Of Translation

No abstract prepared.

1999-11-15

280

Indian Institute of Science Theses & Dissertations (India)

Full Text Available

2004-07-01

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281

Identification of Protective Brucella Antigens and their ...

Heavy Metals Effect on Animal Cells

... Considering these observations, it is most likely that Brucela proteins involved in protective immunity will preferentially stimulate INF-g producing T ...

1996-05-01

282

International Science & Technology Center (ISTC)

Studies of the Superheavy-metal Effect as a Stress-factor on Protein Biosynthesis and Cytoskeleton Integrity in Animal Cells

283

Functional characterization of a CCCH type zinc-finger protein gene OsZF2 by ectopic overexpression of the gene in rice

Free radicals and their transformations in irradiated proteins

Full Text Available

2009-03-01

284

Effect of protein malnutrition on the metabolism of bone collagen in albino rats

Experimental investigation data are systematized of free radical states and processes in irradiated proteins. The investigation is performed by the radiospectral methods. Results are discussed in detail of the study of free radicals electronic structure of amino acids, peptides and proteins formed by the action of ionizing radiation. The specificity is stressed of the study of monocrystalls of these compounds by the method of electronic paramagnetic resonance. The nature is also studied of primary centres formed under the effect of radiation on biologically important compounds and their subsequent reactions in solid and liquid solutions. Ion-radical states of different functional groups of the protein molecule are studied. Prospects of the study and the role of anion-radicals in biological processes are discusses.

285

Effect of Carbon Tetrachloride on the Changes of Xanthine Oxidase Activity in Rate Previously Fed Low or High Protein Diet

The effect of protein malnutrition on the metabolism of collagen in bone was studied in young female albino rats after a single injection of "3H-proline. Both specific and total radioactivities of hydroxyproline in the total collagen of the bone were found to decrease in the protein-deficient animals, indicating decreased rate of collagen synthesis. In the urine the amount of hydroxyproline excreted and total radioactivity of "3H-hydroxyproline were greatly decreased. The results of the present investigation therefore clearly indicate decreased synthesis and catabolism of collagen in bones of protein deficient animals compared to controls. (auth.).

1981-01-01

286

Understanding collaborative filtering parameters for personalized recommendations in e-commerce

Full Text Available

1991-12-01

287

British Library Electronic Table of Contents (United Kingdom)

Collaborative Filtering (CF) is a popular method for personalizing product recommendations for e-Commerce and customer relationship management (CRM). CF utilizes the explicit or implicit product evaluation ratings of customers to develop personalized recommendations. However, there has been no in-depth investigation of the parameters of CF in relation to the number of ratings on the part of an individual customer and the total number of ratings for an item. We empirically investigated the relationships between these two parameters and CF performance, using two publicly available data sets, EachMovie and MovieLens. We conducted three experiments. The first two investigated the relationship between a particular customer?s number of ratings and CF recommendation performance. The third experim...

2007-01-01

288

Tensions of network security and collaborative work practice: Understanding a single sign-on deployment in a regional hospital

British Library Electronic Table of Contents (United Kingdom)

Background: Healthcare providers and their IT staff, working in an effort to balance appropriate accessibility with stricter security mandates, are considering the use of a single network sign-on approach for authentication and password management. Single sign-on (SSO) promises to improve usability of authentication for multiple-system users, increase compliance, and help curb system maintenance costs. However, complexities are introduced when SSO is placed within a collaborative environment. These complexities include unanticipated workflow implications that introduce greater security vulnerability for the individual user. Objectives and methodology: In this work, we examine the challenges of implementing a single sign-on authentication technology in a hospital environment. The aim of the...

2011-01-01

289

Overview of the recent activities of the RD50 collaboration on radiation hardening of semiconductor detectors for the sLHC

Overview of the recent activities of the RD50 collaboration on radiation hardening of semiconductor detectors for the sLHC

The RD50 collaboration has been exploring the development of radiation hard semiconductor devices for very high-luminosity colliders since 2002. The target fluence to qualify detectors set by the anticipated dose for the innermost tracking layers of the future upgrade of the CERN large hadron collider (LHC) is 1016 1 MeV neutron equivalent (neq) cm-2. This is about an order of magnitude higher than the maximum dose for the most exposed silicon detectors in the current machine. RD50 investigates the radiation hardening of silicon sensors from many angles: improvement of the intrinsic tolerance of the substrate material, optimisation of the readout geometry and study of novel design of detectors. A review of some of the recent activities within RD50 is here presented.

2009-01-01

290

British Library Electronic Table of Contents (United Kingdom)

The RD50 collaboration has been exploring the development of radiation hard semiconductor devices for very high-luminosity colliders since 2002. The target fluence to qualify detectors set by the anticipated dose for the innermost tracking layers of the future upgrade of the CERN large hadron collider (LHC) is 1016 1MeV neutron equivalent (neq) cm-2. This is about an order of magnitude higher than the maximum dose for the most exposed silicon detectors in the current machine. RD50 investigates the radiation hardening of silicon sensors from many angles: improvement of the intrinsic tolerance of the substrate material, optimisation of the readout geometry and study of novel design of detectors. A review of some of the recent activities within RD50 is here presented.

2009-01-01

291

Oppenheimer&Groves : The duality that led to Trinity /.

Government-industry-education collaboration for intelligent tutor computer labs

The alliance of J. Robert Oppenheimer, scientist, and Leslie R. Groves, military leader, is often interpreted as the classic example of the clash between the academic mind and the military style. Evidence suggests, instead, that it was a collaboration that led to the dawn of the nuclear age. Instead of a clash, it was collaboration and an implosion of the diverse talents needed for the success of this project. Discussion of these flawed and fascinating individuals still ignites controversy today. This presentation will explore the backgrounds and personalities of these two men and their work together to accomplish their mission. Was the aftermath inevitable, given a relationship based on respect, but perhaps not trust? The genesis of the modern military-industrial complex rested on the genius of these two men, though they personify two distinct American sub-cultures. What lessons can be drawn from their wartime and post-war relationship? What ...

2001-01-01

292

Final ROI Report - Technology Transfer of Waste-Reducing Groundwater Sampling Systems

Through the dedicated collaborative efforts of many individuals interested in supporting local education, computer laboratories were established at two urban high schools. The purchasing and implementation of the project was handled by the Alliance for Education, a local non-profit education advocate. Funds were supplied by the Air Force as part of a 3-year research activity utilizing artificial intelligence technology to tutor 9th grade students. NCR (now AT7T Global Information Solutions) corporate leaders provided the computers and network equipment at a considerable savings to the project. Each lab is a state-of-the-art facility with air-conditioning, carpeting, special computer tables, computer projection screens, and on-site technical support. Student and teacher enthusiasm toward the project has been gratifying and quantitative results are currently being evaluated for both attitude, skills, and state proficiency tests.

1994-12-31

293

EBIT - Electronic Beam Ion Trap: N Divison experimental physics annual report 1995

This report presents the findings of a U.S. DOE Environmental Management technology transfer initiative of waste-reducing ground water sampling systems between Savannah River Site (SRS) and Lawrence Livermore National Laboratory (LLNL) which occurred during fiscal years 2001 and 2002. The report describes the collaboration between the two sites, the deployment of the Savannah River Site Purge Water Management System at LLNL, the changes made to that system for use at LLNL, and documents the return-on-investment derived from the system's use at LLNL as well as other benefits generated through this inter-laboratory collaboration. An evaluation of the deployment of the LLNL EasyPump sampling technology at SRS will be covered in a separate report from SRS.

2002-09-30

294

Collaborative e-product development and product innovation in a demand-driven network: the moderating role of eCRM

The multi-faceted research effort of the EBIT (Electron Beam Ion Trap) program in N-Division of the Physics and Space Technology Department at Lawrence Livermore National Laboratory (LLNL) continues to contribute significant results to the physical sciences from studies with low energy very highly charged heavy ions. The EBIT program attracts a number of collaborators from the US and abroad for the different projects. The collaborations are partly carried out through participating graduate students demonstrating the excellent educational capabilities at the LLNL EBIT facilities. Moreover, participants from Historically Black Colleges and Universities are engaged in the EBIT project. This report describes EBIT work for 1995 in atomic structure measurements and radiative transition probabilities, spectral diagnostics for laboratory and astrophysical plasmas, ion/surface interaction studies, electron-ion interactions studies, retrap and ion ...

1996-10-01

295

British Library Electronic Table of Contents (United Kingdom)

Customer relationships, product development, data integration and demand-driven supply chains are key concepts for companies that wish to remain competitive in today?s global economy. To address this important information management issue, the present study examines how, within a demand-driven network context, an eCRM can influence the interorganizational product development process between a manufacturer and its key customers. The theoretical model, built on research related to a technology assimilation model based on Fichman?s (2000) work, was tested on data from 104 manufacturers in the wireless equipment sector. The results show that the adoption of an eCRM does not moderate the relationship between collaborative e-product development involving a manufacturer and its key customers and ...

2009-01-01

296

Collaborative Search Trails for Video Search

(International Panel on 14 MeV Intense Neutron Source Based on Accelerators for Fusion Materials Study)

In this paper we present an approach for supporting users in the difficult task of searching for video. We use collaborative feedback mined from the interactions of earlier users of a video search system to help users in their current search tasks. Our objective is to improve the quality of the results that users find, and in doing so also assist users to explore a large and complex information space. It is hoped that this will lead to them considering search options that they may not have considered otherwise. We performed a user centred evaluation. The results of our evaluation indicate that we achieved our goals, the performance of the users in finding relevant video clips was enhanced with our system; users were able to explore the collection of video clips more and users demonstrated a preference for our system that provided recommendations.

2009-01-01

297

Turnover of whole body proteins and myofibrillar proteins in middle-aged active men

Both travelers were members of a nine-person US delegation that participated in an international workshop on accelerator-based 14 MeV neutron sources for fusion materials research hosted by the University of Tokyo. Presentations made at the workshop reviewed the technology developed by the FMIT Project, advances in accelerator technology, and proposed concepts for neutron sources. One traveler then participated in the initial meeting of the IEA Working Group on High Energy, High Flux Neutron Sources in which efforts were begun to evaluate and compare proposed neutron sources; the Fourth FFTF/MOTA Experimenters' Workshop which covered planning and coordination of the US-Japan collaboration using the FFTF reactor to irradiate fusion reactor materials; and held discussions with several JAERI personnel on the US-Japan collaboration on fusion reactor materials.

1991-02-14

298

Tomato 14-3-3 Protein 7 Positively Regulates Immunity-Associated Programmed Cell Death by Enhancing Protein Abundance and Signaling Ability of MAPKKK ?^[C]^[W]

Endurance-trained older men have a higher proportion of lean tissue and greater muscle cell oxidative capacity, reversing age-related trends and suggesting major changes in protein metabolism. In this study, protein turnover was determined in 6 middle-aged (52+/-1 yr) men who were well trained (VO_2 max 55.2+/-5.0 ml O_2/kg.min) and lean (body fat 18.9+/-2.8%, muscle mass 36.6+/-0.6%). The maintained habitual exercise while consuming 0.6, 0.9 or 1.2 g protein/kg.day for 10-day periods. N flux was measured from "1"5N in urea after oral "1"5N-glycine administration. Myofibrillar protein breakdown was estimated from urinary 3-methyl-histidine. Dietary protein had no effect on turnover rates, even when N balance was negative. Whole body protein synthesis was 3.60+/-0.12 g/kg.day and breakdown was 3.40+/-0.14 g/kg.day for all N intakes. Whole body ...

1986-04-13

299

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis^[W]^[OA]

Programmed cell death (PCD) is triggered when Pto, a Ser-Thr protein kinase, recognizes either the AvrPto or AvrPtoB effector from Pseudomonas syringae pv tomato. This...Full Text Available

2010-01-01

300

Scyl1 Facilitates Nuclear tRNA Export in Mammalian Cells by Acting at the Nuclear Pore Complex

In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal...Full Text Available

2010-05-01

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301

Relationships of Quality Characteristics with Size Exclusion HPLC Chromatogram of Protein Extract in Soft-White Winter Wheats.

Scyl1 is an evolutionarily conserved N-terminal protein kinase-like domain protein that plays a role in COP1-mediated retrograde protein trafficking in mammalian cells. Furthermore, loss of Scyl1 function...Full Text Available

2010-07-15

302

Protective Effects of a Human 18-Kilodalton Cationic Antimicrobial Protein (CAP18)-Derived Peptide against Murine Endotoxemia

This study investigated relationships between molecular weight distributions of unreduced grain proteins and grain, flour, and end-use quality characteristics of soft white winter wheats grown in Oregon. Absorbance area and area % values of protein fractions separated by size exclusion HPLC (SE-HPL...

303

Influence of the high-affinity growth hormone (GH)-binding protein on plasma profiles of free and bound GH and on the apparent half-life of GH. Modeling analysis and clinical applications.

CAP18 (an 18-kDa cationic antimicrobial protein) is a granulocyte-derived protein that can bind lipopolysaccharide (LPS) and inhibit various activities of LPS in vitro. The present study examined the...Full Text Available

1998-05-01

304

Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

The discovery of a specific high-affinity growth hormone (GH) binding protein (GH-BP) in plasma adds complexity to the dynamics of GH secretion and clearance. Intuitive predictions are that such a protein...Full Text Available

1993-02-01

305

Gamma interferon induces rapid and coordinate activation of mitogen-activated protein kinase (extracellular signal-regulated kinase) and calcium-independent protein kinase C in human monocytes.

We have identified another Drosophila GTP-binding protein (G protein) alpha subunit, dGq alpha-3. Transcripts encoding dGq alpha-3 are derived from alternative splicing of the dGq alpha locus previously...Full Text Available

1995-12-05

306

Dual Functions of ASCIZ in the DNA Base Damage Response and Pulmonary Organogenesis

Gamma interferon plays an important role in regulating the functional properties of mononuclear phagocytes. In the present study, the role of activated protein kinases in the mechanism of action of...Full Text Available

1994-07-01

307

Chemical and Immunological characterization of the major structural protein (p28) of MMC-1, a rhesus monkey endogenous type C virus: homology with the major structural protein of avian reticuloendotheliosis virus.

Zn²⁺-finger proteins comprise one of the largest protein superfamilies with diverse biological functions. The ATM substrate Chk2-interacting Zn²⁺-finger protein...Full Text Available

2010-10-01

308

Study of protein-protein interactions in under saturated and supersaturated lysozyme solutions in heavy water as a function of temperature; Etude des interactions proteine-proteine en solutions sous-saturees et sursaturees de lysozyme dans l`eau lourde en fonction de la temperature

The major core protein (p28) of MMC-1, an endogenous type C virus of the rhesus monkey (Macaca mulatta), was purified and subjected to structural and immunological analyses. The NH2-terminal amino acid...Full Text Available

1981-09-01

309

Observation of the study of protein metabolism in relation to nutrition

We have studied freshly prepared lysozyme solutions in heavy water for two NaCl concentrations as a function of temperature. Lysozyme solubilities in this solvent are determined by static light scattering. By small angle neutron scattering, we evidence that interactions between lysozyme molecules are characterized by a second virial coefficient A{sub 2} whether the solution is under-saturated or supersaturated. From the variation of A{sub 2} as a function of temperature we have evaluated the enthalpy corresponding to the interaction between lysozyme molecules. We show that the interactions between protein molecules are higher in heavy water than in light water. (authors). 13 refs., 3 figs.

1996-04-01

310

Identification of a functional nuclear export signal in the green fluorescent protein asFP499

It is a useful hypothesis to thing of the reaction to protein deprivation as made up of two parts. First there is an initial adaptation involving rapid losses of protein. This is followed by slow continuing depletion. When considering two organ groups, such as the liver and muscle, it appears that the curve of urinary nitrogen excretion reflects the different parts they play in these two phases. The changes in the relative amounts of protein in different organs results in an alteration in the pattern of body protein at the level of the organ, the tissue and the cell. These changes are themselves the result of altered rates of synthesis and breakdown. The questions that must be asked and hopefully will be answered with the help of isotopes, are how these changes are brought about, how they are inter-related and controlled, and what is their significance? This paper contains a discussion of the few ...

1965-06-01

311

Artificial neural network modeling of physicochemical changes of shrimp during boiling

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

312

British Library Electronic Table of Contents (United Kingdom)

Frozen boiled shrimp and dried shrimp are among the high-value fishery products of Thailand. During the production of these products boiling is one of the most important steps that affects significantly the product physicochemical properties, especially the quantity and quality of proteins, which in turn affect other apparent properties perceived by consumers. The protein changes are, however, difficult to evaluate comparing to other typical physical properties of shrimp. The objective of this study was therefore to develop an artificial neural network (ANN) model to predict the protein changes of shrimp in terms of protein loss and protein denaturation as a function of the boiling conditions, namely, concentration of salt solution and boiling time, as well as a rather easily determined ch...

2012-01-01

313

Amyloid oligomers: spectroscopic characterization of amyloidogenic protein states

British Library Electronic Table of Contents (United Kingdom)

It is assumed that protein fibrils manifested in amyloidosis result from an aggregation reaction involving small misfolded protein sequences being in an `oligomeric' or `prefibrillar' state. This review covers recent optical spectroscopic studies of amyloid protein misfolding, oligomerization and amyloid fibril growth. Although amyloid fibrils have been studied using established protein-characterization techniques throughout the years, their oligomeric precursor states require sensitive detection in real-time. Here, fluorescent staining is commonly performed using thioflavin T and other small fluorescent molecules such as 4-(dicyanovinyl)- julolidine and 1-amino-8-naphtalene sulphonate that have high affinity to hydrophobic patches. Thus, populated oligomeric intermediates and related `pre...

2010-01-01

314

Altered protein phosphorylation in sciatic nerve from rats with streptozocin-induced diabetes

Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells

The effect of experimental diabetes on the phosphorylation of proteins in the rat sciatic nerve was studied. Nerves from animals made diabetic with streptozocin were incubated in vitro with (/sup 32/P)orthophosphate and divided into segments from the proximal to the distal end, and proteins from each segment were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The principal labeled species were the major myelin proteins, P0, and the basic proteins. After 6 wk of diabetes, the incorporation of isotope into these proteins rose as a function of distance along the nerve in a proximal to distal direction and was significantly higher at the distal end compared with incorporation into nerves from age-matched controls. The overall level of isotope uptake was similar in nerves from diabetic animals and weight-matched controls. The distribution of /sup 32/P among ...

1987-11-01

315

Euglena light-harvesting chlorophyll A/B binding protein (LHCP) synthesized as an unusually large precursor

The purpose of the present study was to examine the in vitro effects of low-dose cadmium (Cd) on developing cortical cells. The cortical cells removed from fetuses (embryonic day 15) were treated with 10 nM of Cd for 24 h. The effects of Cd on dendritic and synaptic development were immunocytochemically observed with anti-microtubule associated protein-2 (MAP2) and anti-synapsin I antibodies, respectively. Administration of Cd suppressed dendritic as well as synaptic development at 10 nM. By two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometric (LC/MS/MS) analysis, we identified three proteins with different expression after Cd-treatment; dihydropyrimidinase-related protein 2 (DRP-2/CRMP-2), 14-3-3-epsillon and calmodulin (CaM). Though the number of identified proteins was small, these proteins are known to be involved in neuronal development. The ...

2008-11-20

316

Remote participation technical infrastructure for the JET facilities under EFDA

Light increased the rate of LHCP synthesis as measured by pulse-labeling with /sup 35/SO/sub 4/ and immunoprecipitation with antibody specific for Euglena LHCP. In addition to the mature LHCP, 26,000 daltons, the LHCP specific antibody immunoprecipitated large amounts of several proteins having molecular weights of approximately 100,000. On immunoblots of immunoprecipitated unlabeled protein, the antibody only detected the mature LHCP suggesting that the high molecular weight proteins are not LHCP aggregates produced during immunoprecipitation. After a 10 min pulse with /sup 35/SO/sub 4/, the 100,000 dalton proteins constituted over 80% of the immunoprecipitated material. In a subsequent chase, the radioactivity in the 100,000 dalton proteins decreased and the radioactivity in the mature LHCP increased suggesting a precursor-product relationship. After a 35 minute chase, the mature ...

1987-04-01

317

Quality controls in radiotherapy. From experiences of the first course-debate

The new collaborative exploitation of the JET Facilities requires suitable technical tools and infrastructure measures to enable the Remote Participation of scientists from all European Fusion labs. Such measures are being gradually introduced. They comprise of a toolkit for Remote Data Access, Remote Computer Access and for teleconferencing, and of infrastructure measures that address network connectivity, network security, shared documentation and technical support.

2001-10-01

318

Producing Open Source Software How to Run a Successful Free Software Project

The document reports the main issues discussed during the first course-debate on quality controls in radiotherapy, held in Rome in December 3-4, 1998 and May 3-5 1999 in collaboration between the Istituto Superiore di Sanita' and the Istituto Regina Elena. The points considered critical to the quality assurance in radiotherapy by the participants are presented in relation to the total radiotherapy procedure.

1998-12-03

319

Piperazine sulfonamide BACE1 inhibitors: Design, synthesis, and in vivo characterization

This comprehensive guide offers several tried and true steps to help you successfully manage the complex process of developing free software. Topics include project management, developer motivation, technical infrastructure to support collaboration, and project promotion. Producing Open Source Software is ideal for developers starting their own free software projects, or people who simply want to participate in the process.

2009-01-01

320

British Library Electronic Table of Contents (United Kingdom)

With collaboration between chemistry, X-ray crystallography, and molecular modeling, we designed and synthesized a series of novel piperazine sulfonamide BACE1 inhibitors. Iterative exploration of the non-prime side and S2prime sub-pocket of the enzyme culminated in identification of an analog that potently lowers peripheral Ab40 in transgenic mice with a single subcutaneous dose.

2010-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

PV Conversion Technologies, Session: OPV, Sensitized, Seed (Presentation)

Nuclear methods monitor nutrition

The NREL Sensitized Solar Cell (SSC) Core Program supports the Solar America Initiative by: (1) targeting new devices and processes for commercialization by 2015 that are less expensive, more efficient, highly reliable, and environmentally benign; (2) collaborating with DOE OS/BES to conduct basic research targeting breakthroughs in key areas, such as ultra-high efficiency and/or ultra-low cost materials and devices.

2008-04-01

322

Neutron activation of nitrogen and hydrogen in the body, the isotope dilution technique and the measurement of naturally radioactive potassium in the body are among the new nuclear methods, now under collaborative development by the Australian Nuclear Scientific and Technology Organization and medical specialists from several Sydney hospitals. These methods allow medical specialists to monitor the patient's response to various diets and dietary treatments in cases of cystic fibrosis, anorexia nervosa, long-term surgical trauma, renal diseases and AIDS. ills.

323

NAME=\\

INL Advanced Radiotherapy Research Program Annual Report 2004

... This study catalysed a more comprehensive collaborative research programme, Assessment of the durability and engineering properties of lesser known hardwood timber species for use in marine and freshwater construction, jointly funded by the Environment Agency and TRADA, and managed by H R Wallingford. Additional funding was provided by British Waterways, The Crown Estate, CETMEF (Centre d'Etudes Techniques Maritimes et Fluviales) and VolkerStevin Ltd. Timber ...

324

This report summarizes the activities and major accomplishments for the Idaho National Laboratory Advanced Radiotherapy Research Program for calendar year 2004. Topics covered include boron analysis in biological samples, computational dosimetry and treatment planning software development, medical neutron source development and characterization, and collaborative dosimetry studies at the RA-1 facility in Buenos Aires, Argentina.

2005-06-01

325

High energy physics

Flexible DER Utility Interface System: Final Report, September 2004--May 2006

The research of the CELLO collaboration is reviewed. After a description of the CELLO detector tests of QCD and hadronic final states by determination of the strong coupling constant and studies of inclusive production of electrons and muons in multihadronic events in e"+e"- annihilation are described. Then studies of deep inelastic e#gamma# scattering with lepton pair production and the study of the structure function of the photon are briefly described. Finally studies on QED processes are described together with tau decays. (HSI).

1983-11-01

326

Enabling Technologies for Petascale Electromagnetic Accelerator Simulation

In an effort to accelerate deployment of Distributed Energy Resources (DER) such as wind, solar, and conventional backup generators to our nation's electrical grid, Northern Power Systems (NPS), the California Energy Commission (CEC), and the National Renewable Energy Laboratory (NREL) collaborated to create a prototype universal interconnect device called the DER Switch.

2006-08-01

327

Effect of lifetime nutrition on ruminant carcass and meat quality

The SciDAC2 accelerator project at SLAC aims to simulate an entire three-cryomodule radio frequency (RF) unit of the International Linear Collider (ILC) main Linac. Petascale computing resources supported by advances in Applied Mathematics (AM) and Computer Science (CS) and INCITE Program are essential to enable such very large-scale electromagnetic accelerator simulations required by the ILC Global Design Effort. This poster presents the recent advances and achievements in the areas of CS/AM through collaborations.

2007-11-09

328

DescriptionThis application is a request for Bridge Link funds to support studies in order to develop a full proposal under the Link Sustainable Livestock Scheme in collaboration with Dr Nigel Scollan of IGER. The hypothesis being examined is that the poor performance of some ruminants is due to poor nutrition at strategic time points during the animal's development. The initial hypothesis will be tested in sheep and the results from this will be used to develop the full proposal. Excessive fat in ruminan [continued...

2006-01-31

329

Duality rotations

Determining the upper limit of Gamma_{ee} for the Y(4260)

Irrespective of supersymmetry, it is interesting to understand the special properties of theories admitting duality rotations. The Lagrangian of such a theory is not invariant under the transformations, nor does it change by a total derivative, but it transforms in a particular way which implies that the system of the equations of motion is invariant and that observables, such as the energy momentum tensor and therefore the total energy and momentum, are invariant. The main results of a recent paper on the properties of theories admitting duality rotations written in collaboration with M. K. Gaillard (1981) are described.

1981-11-01

330

Communicating Mathematics on the Internet: Synchronous and Asynchronous Tools

By fitting the R values between 3.7 and 5.0 GeV measured by the BES collaboration, the upper limit of the electron width of the newly discovered resonance Y(4260) is determined to be 240 eV at 90% C.L., together with the BABAR measurement on the product of Gamma_{ee} and BR(Y(4260) --> pi+pi- J/psi), this implies a large decay width of Y(4260) --> pi+pi- J/psi final states.

2006-01-01

331

British Library Electronic Table of Contents (United Kingdom)

Communicating and collaborating online are becoming common requirements in education. The specialized notations and symbols necessary for some content areas where mathematical expressions are part of the conversation require that users select appropriate communication tools. The purpose of this article is to describe tools that can be used for online, asynchronous or synchronous communication involving mathematical expressions.

2011-01-01

332

Coastal conservation: essays on experiments in governance. [Monograph

Charmonium with three flavors of synamical quarks

Three book reviews and five essays address the problems of governing coastal regions. The books reviewed and the articles covered the institutions and programs involved in resource management, the balance of local and state interests in coastal conservation and developments, a consensus on coastal planning issues, and a growing trend in state-local collaborative planning. Four of the articles pertain to California, and one focuses on programs in Maine, Rhode Island, and Washington. 135 references. (DCK)

1981-01-01

333

B(s) properties at the Tevatron

We present a calculation of the charmonium spectrum with three flavors of dynamical staggered quarks from gauge configurations that were generated by the MILC collaboration. We use the Fermilab action for the valence charm quarks. Our calculation of the spin-averaged 1P-1S and 2S-1S splittings yields a determination of the strong coupling, with {alpha}{sub {ovr MS}}(M{sub Z}) = 0.119(4).

2003-12-23

334

The Tevatron collider at Fermilab provides a very rich environment for the study B{sub s} mesons. In this paper they show a few selected topics from the CDF and D0 collaborations, giving special attention to the B{sub s} Mixing analyses. This note corresponds to the proceedings of the Hadron Collider Physics 2005 conference.

2005-11-01

335

Annual report 1993-1994

Analysis of the MEX-15 multipurpose reactor using SRAC code system

The Atlantic Wind Test Site was established in 1980 as a laboratory to facilitate the evaluation and demonstration of wind energy systems and equipment. This annual report describes its organization and management, facilities, major projects, and the outlook for the future. Major projects include wind diesel; AWTS-IREQ collaboration; remote community data acquisition; lagerwey test program; Alaska Energy Authority Project; public education; Atlantic Orient Corporation 15/50 Project; and the soft VAWT project.

1994-12-31

336

Activity report 2001; Rapport annuel d'activite 2001

The MEX-15 is a conceptual design of a Multipurpose Reactor with thermal power of 15 MW and this reactor is pool type with fuel plates U{sub 3}0{sub 8}-Al of low enrichment uranium. This report presents the static calculation for the MEX-15 reactor using SRAC code system and was developed under the collaboration agreement between ININ-JAERI in Research Reactor Technology Development Division of Department of Research Reactor in Tokai Research Establishment. (Author)

1992-12-15

337

Abdominal masses in the pediatric age. Characterization by CT guided fine needle aspiration biopsy

The DED, Department of the Wastes Studies, part of the Direction of the Nuclear Energy of the CEA, regroups knowledge in the domain of the chemistry, the biochemistry, the process measures and simulation engineering applied to the radioactive wastes management. This annual report presents for the year 2001 the missions, the organization, the scientific collaborations, the research programs and the publications of the Department. (A.L.B.)

2001-07-01

338

ATLAS tile calorimeter electronics upgrade

CT-guided fine needle aspiration biopsy (FNAB) is known to improve diagnosis of expansive abdominal lesions, especially relative to more invasive procedure like explorative laparotomy. FNAB in not commonly use in pediatric patients because of their poor collaboration and of associated risks. The authors investigated the feasibility of FNAB in the pediatric age.

1999-04-01

339

ADS as Information Management Service in an M-Learning Environment

This presentation summarizes the present upgrade R&D projects in the TileCal collaboration. One project studies the possibility to divide the drawers into smaller minidrawers to simplify maintenance. There are three different front-end projects. There are also projects dealing with high voltage and low voltage distribution, projects for developing PMT dividers, low voltage electronics, data transmission and off-detector electronics.

2011-01-01

340

A Search for electron neutrino appearance at the Delta m**2 ~ 1- eV**2 scale

Leveraging the potential power of even small handheld devices able to communicate wirelessly requires dedicated support. In particular, collaborative applications need sophisticated assistance in terms of querying and exchanging different kinds of data. Using a concrete example from the domain of mobile learning, the general need for information dissemination is motivated. Subsequently, and driven by infrastructural conditions, realization strategies of an appropriate middleware service are discussed.

2007-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

The Effects of Surface Chemistry on the Properties of Proteins Confined in Nano-porous Materials

The MiniBooNE Collaboration reports first results of a search for {upsilon}{sub e} appearance in a {upsilon}{sub {mu}} beam. With two largely independent analyses, we observe no significant excess of events above background for reconstructed neutrino energies above 475 MeV. The data are consistent with no oscillations within a two neutrino appearance-only oscillation model.

2007-04-01

342

Single protein omission reconstitution studies of tetracycline binding to the 30S subunit of Escherichia coli ribosomes

The entrapment of proteins using the sol-gel route provides a means to retain its native properties and artificially reproduce the molecular crowding and confinement experienced by proteins in the cell allowing investigation of the physico-chemical and structural properties of biomolecules at the biotic/abiotic interface. The biomolecules are spatially separated and 'caged' in the gel structure but solutes can freely permeate the matrix. Thus, properties such as the folding of ensembles of individual molecules can be examined in the absence of aggregation effects that can occur in solution studies. Green fluorescent protein from Aequorea coerulescens was used as a model protein to examine the unfolding/re-folding properties of protein in silica gels. The recombinant protein was isolated and purified from Escherichia coli extracts by cell lysis, ...

2007-09-01

343

p53 protein in aggressive and non-aggressive basal cell carcinoma.

In previous work the authors showed that on photolysis of Escherichia coli ribosomes in the presence of ({sup 3}H)tetracycline (TC) the major protein labeled is S7, and they presented strong evidence that such labeling takes place from a high-affinity site related to the inhibitory action of TC. In this work they use single protein omission reconstitution (SPORE) experiments to identify those proteins that are important for high-affinity TC binding to the 30S subunit, as measured by both cosedimentation and filter binding assays. With respect to both sedimentation coefficients and relative Phe-tRNA{sup Phe} binding, the properties of the SPORE particles they obtain parallel very closely those measured earlier, with the exception of the SPORE particle lacking S13. A total of five proteins, S3, S7, S8, S14, and S19, are shown to be important for TC binding, with the largest effects seen on omission of ...

1990-06-05

344

Proteomic Analysis of Proteins Secreted by HepG2 Cells Treated with Butyl Benzyl Phthalate

Basal cell carcinoma (BCC) is the most frequent cutaneous neoplasm, with a generally favorable clinical behavior. Sometimes, indeed, it recurs after therapy and/or metastasizes. As point mutations in the coding sequence of the p53 tumor suppressor gene have been implicated in the progression of many human tumors, we studied the expression of p53 protein on this neoplasia. We tested immunohistochemically the positivity for p53 protein (NCL-p53-CM1, YLEM) on 19 cases of morphologically "non aggressive" BCC (BCC1) and on 19 "aggressive" BCC (BCC2), all with one or more relapses and 3 with distant metastases also. Results were related to clinico-pathological and follow-up data. All but one BCC2 were found positive for p53 protein. Conversely, only 2 cases of BCC1 exhibited low immunoreactivity for p53 protein, with high statistical differences between the two groups. No correlation was found between the ...

1993-10-01

345

British Library Electronic Table of Contents (United Kingdom)

Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 ?M) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate ...

2010-01-01

346

Macronutrient self-selection in Solea senegalensis fed macronutrient diets and challenged with dietary protein dilutions

British Library Electronic Table of Contents (United Kingdom)

Macronutrient self-selection and responses to dietary protein dilution were investigated in Senegalese sole, a flatfish species of interest for Mediterranean aquaculture. Eight groups of six sole were distributed among 75-l tanks and allowed to feed on three experimental diets composed of pure macronutrients: PC (75% crude protein and 25% carbohydrate), PF (75% crude protein and 25% crude fat) and PFC (10% crude protein, 45% crude fat and 45% carbohydrate). Choosing from the above diets, the sole selected a diet containing 68.0% crude protein, 15.7% crude fat and 16.3% carbohydrate. When the PF diet was diluted 50% with cellulose, the fish increased their feed intake to sustain the energy intake (5.0?kJ/kg BW/day). Their selection of fat remained unchanged, while carbohydrate selection sig...

2009-01-01

347

Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori

British Library Electronic Table of Contents (United Kingdom)

Expressed sequence tags (ESTs) of the maxillary galea of the silkworm were analyzed to identify proteins involved in food selection systems. From the 1251 redundant genes of the ESTs, we identified 7 odorant-binding protein-like genes (bmObpL), 6 takeout-like genes (bmToL), and 6 chemosensory protein genes (bmCsp). Quantitative RT-PCR analysis indicated that bmObpL1, bmObpL2, bmObpL3, bmObpL5, bmToL1, bmToL3, and bmorCsp15 were predominantly expressed in the larval oral appendages, such as the maxilla, labrum, labium and antenna. Immunocytochemical analysis indicated that the proteins of bmObpL1, bmObpL3, and bmToL1 were localized in the gustatory chemosensilla on the maxillary galea and olfactory sensilla in the antenna. The proteins encoded by bmObpL1 and bmObpL3 were detected in the gus...

2011-01-01

348

Human complement protein C99 is a calcium binding protein

Human complement protein C9 is shown to be a metalloprotein that binds 1 mol of Ca"2"+/mol of C9 with a dissociation constant of 3 #mu#m as measured by equilibrium dialysis. Incubation with EDTA removes the bound calcium, resulting in a apoprotein with decreased thermal stability. This loss in stability leads to aggregation and, therefore, to loss of hemolytic activity upon heating to a few degrees above the physiological temperature. Heat-induced aggregation of apoC9 can be prevented by salts that stabilize proteins according to the Hofmeister series of lyotropic ions, suggesting that the ion in native C9 may ligand with more than one structural element of domain of the protein. Ligand blotting indicates that the calcium binding site is located in the amino-terminal half of the protein. Removal of calcium by inclusion of EDTA in assay mixtures has no effect on the hemolytic activity of C9, and its ...

349

Amyloidosis

A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding

The subjects covered in this Symposium range through almost every clinical medical specialty. From an average of one paper in each of the past three Symposiums, the explosive interest in cerebral amyloidosis has led to the presentation of 12 papers on this subject in the present volume. The genetically predisposed familial amyloidotic processes, such as the polyneuropathies and familial Mediterranean fever have also stimulated extensive and intriguing investigations which have revealed the striking effect of a single amino acid substitution in transforming a normal protein into a lethal ''amyloidogenic'' one. This Symposium clearly depicts the advances since the first amyloid fibril protein was definitively identified and defined 14 years ago. Since all amyloid fibril proteins so far described are variants of normal proteins, attention to gene abnormalities now becomes a significant focus as well as the ...

1984-11-09

350

Vibrational Circular Dichroism: A developing spectroscopic technique for peptide and protein conformational studies

The authors have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative amino acid substitution (cysteine to phenylalanine) at codon 706. Stable expression of this mutant RB1 cDNA in a human cell line lacking endogenous RB1 demonstrated that this amino acid change was sufficient to inhibit phosphorylation. In addition, this cysteine-to-phenylalanine substitution also resulted in loss of RB1 binding to the simian virus 40 large tumor and adenovirus E1A transforming proteins. These results confirm the importance of exon 21 coding sequences and suggest that the cysteine residue at codon 706 may play a role in achieving a specific protein conformation essential for ...

351

Fluoride induced changes in protein metabolism in the tissues of freshwater crab Barytelphusa guerini.

Electronic circular dichroism (ECD) of the n-#pi# and #pi# -#pi# transitions of the amide groups in the ultraviolet has become an indispensable tool for qualitative characterization of proteins in solution. Since the existence of such a spectrum (that is, of natural optical activity) comes from the three dimensional interaction of the chromophores in the molecule, CD is exquisitely sensitive to molecular conformation. However, in proteins, the amide transitions available in the near UV are limited in number and are broads and overlapping. Interactions among them yield information about the polymeric backbone, but since such amide electronic excitation are relatively delocalized, the resulting

1996-11-01

352

Cysteine-containing peptides having antioxidant properties

Exposure of the freshwater field crab Barytelphusa guerini to a sublethal concentration of NaF (30 mg litre(-1)) caused significant alterations in protein metabolism. After an exposure time of 15 days, the crab was found to have a marked depletion of total protein and free amino acid content. A significant elevation in transaminases, Aspartate aminotransferase (AAT) and Alanine aminotransferase (ALAT), and Glutamate dehydrogenase (GDH) activities was reflected in the free amino acid levels of the tissues. PMID:15092215

1990-01-01

353

Wnt/b-catenin signal pathway stabilizes APP intracellular domain (AICD) and promotes its transcriptional activity

The term "homology" or "homologous" means an amino acid similarity measured by the program, BLAST (Altschul et al (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:33 89 3402), and expressed as --(% identity n/n). In measuring homology between a peptide and a protein of greater size, homology is measured only in the corresponding region; that is, the protein is regarded as only having the same general length as the peptide, allowing for gaps and insertions.

2007-05-15

354

British Library Electronic Table of Contents (United Kingdom)

Amyloid precursor protein (APP), a key protein in pathogenesis of Alzheimer's disease (AD), is a type I transmembrane protein which can be cleaved by b- and g-secretase to release the amyloidogenic b-amyloid peptides (Ab) and the APP intracellular domain (AICD). While Ab has been widely believed to initiate pathogenic cascades culminating AD, the physiological functions and regulations of AICD remain elusive. In present study, endogenous AICD was demonstrated to be increased by canonical Wnt signal. Instead of due to g-secretase activity, enhanced AICD expression was found due to the increased protein stability by Wnt/b-catenin. b-Catenin was demonstrated to be an associating partner of AICD, capable of promoting AICD mediated transcriptional activity. Investigation by AICD mutants proved ...

2011-01-01

355

The actin content of fibroblasts.

Tau inhibits tubulin oligomerization induced by prion protein

Cultures of chick skin fibroblasts were dissolved in solutions of sodium dodecyl sulphate, and their entire protein content was examined by gel electrophoresis. The most abundant species migrated in...Full Text Available

1975-05-01

356

British Library Electronic Table of Contents (United Kingdom)

In previous studies we have demonstrated that prion protein (PrP) interacts with tubulin and disrupts microtubular cytoskeleton by inducing tubulin oligomerization. These observations may explain the molecular mechanism of toxicity of cytoplasmic PrP in transmissible spongiform encephalopathies (TSEs). Here, we check whether microtubule associated proteins (MAPs) that regulate microtubule stability, influence the PrP-induced oligomerization of tubulin. We show that tubulin preparations depleted of MAPs are more prone to oligomerization by PrP than those containing traces of MAPs. Tau protein, a major neuronal member of the MAPs family, reduces the effect of PrP. Importantly, phosphorylation of Tau abolishes its ability to affect the PrP-induced oligomerization of tubulin. We propose that t...

2011-01-01

357

Stochastic gene expression and its consequences

Site-Specific Methylation of the Promoter Alters Deoxyribonucleic Acid-Protein Interactions and Prevents ...

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

358

Refilling Intracellular Calcium Stores

... and R. L. Momparler. DNA methylation of retinoic acid receptor beta in breast cancer and possible therapeutic role of ... ...

359

Proteomic Analysis of Global Changes in Protein Expression During Exposure of Gamma Radiation in Bacillus sp. HKG 112 Isolated from Saline Soil

Within the cardiac cell, the movements of calcium ions are tightly regulated by a number of regulatory proteins including pumps, and channels. The sarcoplasmic reticulum (SR) is in large part...Full Text Available

2010-01-01

360

Protein expression profile from the Mesocestoides corti (Platyhelminthes: Cestoda) larval stage

Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Pressure Stabilization of Proteins from Extreme Thermophiles

English 2007 [1 p.] Brazil Laschuk, A. Silveira, PA da Bizarro, CV Carvalho,

2007-05-21

362

Possible Implications of Placental Expressions for Heat Shock Protein 70 and Lectin-like Oxidized Low Density Lipoprotein Receptor-1 on Postnatal Blood Pressure in Their Newborns

We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published...Full Text Available

1994-03-01

363

Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin

Full Text Available

2007-09-01

364

Phylogenomic analyses of malaria parasites and evolution of their exported proteins

Eukaryotic genomes are packaged in two basic forms, euchromatin and heterochromatin. We have examined the composition and organization of Drosophila melanogaster heterochromatin in...Full Text Available

2011-02-01

365

Phosphorylation of Annexin A1 by TRPM7 Kinase: A Switch Regulating the Induction of an ?-Helix

BackgroundPlasmodium falciparum is the most malignant agent of human malaria. It belongs to the taxon Laverania, which includes other ape-infecting Plasmodium...Full Text Available

366

PUTATIVE CSF PROTEIN BIOMARKER CANDIDATES FOR AMNESTIC MILD COGNITIVE IMPAIRMENT

TRPM7 is an unusual bifunctional protein consisting of an α-kinase...Full Text Available

2011-03-29

367

PHENOTYPIC MODIFICATION OF ROACH (RUTILUS RUTILUS L.) INFECTED WITH LIGULA INTESTINALIS L. (CESTODA: PSEUDOPHYLLIDEA)

The identification of individuals at risk for Alzheimer's disease (AD) is essential for the timely administration of treatment approaches aimed at slowing the onset or progression of the disease....Full Text Available

2010-03-01

368

Ovine reference materials and assays for prion genetic testing

... growth hormone-like factor from plerocercoids of the tapeworm Spirometra mansonoides is a multifunctional protein. In Parasites and pathogens: ... ...

369

BackgroundGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests...Full Text Available

370

Orphan GPCR research

Mutations in RNA Binding Protein Gene Cause Familial Dilated Cardiomyopathy

Orphan G protein-coupled receptors (GPCRs) are receptors lacking endogenous ligands. Found by molecular biological analyses, they became the roots of reverse pharmacology, in which receptors are attempted...Full Text Available

2008-03-01

371

Liver Carcinogenesis Unit

ObjectivesWe sought to identify a novel gene for dilated cardiomyopathy (DCM).BackgroundDCM is a heritable, genetically...Full Text Available

2009-09-01

372

Inhibition of heat shock protein 27 (HspB1) tumorigenic functions by peptide aptamers

The Liver Carcinogenesis Section uses the modern technologies of molecular biology, cell biology, protein chemistry and genetics to investigate the biochemical and genetic pathways involved in human liver cancer.

373

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

2011-01-01

374

Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin

Identification of Contractile Vacuole Proteins in Trypanosoma cruzi

The tetrapeptide KDEL is commonly found at the C terminus of soluble proteins of the endoplasmic reticulum (ER), and it contributes to their localization by interacting with a receptor that recycles...Full Text Available

2001-05-01

375

Hydrophobic labelling of membrane-embedded proteins with lipophilic reagents

Contractile vacuole complexes are critical components of cell volume regulation and have been shown to have other functional roles in several free-living protists....Full Text Available

376

High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead

Hydrophobic labelling is frequently used in the study of membrane-inserted domains of intrinsic proteins. However, the published procedures, fail to incorporate sufficient radioactivity into membrane immunoglobulins of B lymhocytes to permit investigation of their subunit structures and associations with other proteins. In order to increase the specific radioactivity of ["1"2"5I]iodonaphtylazide ["1"2"5I]INA), an improved method for the synthesis of the reagent was developed. In addition, the optimal conditions for labelling B lymhpocytes with ["1"2"5I]INA and commercially available reagent 3-(trifluoromethyl)-3-(trifluoromethyl)-3-(3'-["1"2"5Iliodophenyl)diazirine (["1"2"5I]TID were isolated and analysed in detail by SDS-PAGE. The usefulness of the two reagents for the investigation of lipid-embedded domains of membrane proteins is discussed. (author). 28 refs.; 4 figs.

377

High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead

The PbII binding characteristics of the previously reported PbII binding proteins of rat kidney cytosol were investigated further. Saturation and Scatchard analysis of /sup 203/Pb binding in whole cytosol and in 40% saturated ammonium sulfate precipitated fractions disclosed a class of relatively high-affinity sites with an apparent Kd of approximately 50 nM and binding capacities of approximately 41 and 9 pmol/mg of protein, respectively. Two /sup 203/Pb binding proteins with approximate molecular masses of 63K and 11.5K daltons and a high molecular weight component (greater than 200K) were isolated by Sepharose-6B column chromatography. The time course of association of /sup 203/Pb with cytosol and the 63K protein showed maximum binding at 18 hr which was stable up to 25 hr at 4 degrees C. The approximate half-time dissociation rate (T 1/2) of specifically bound /sup 203/Pb to the 63K ...

1985-02-01

378

HDL in humans with cardiovascular disease exhibits a proteomic signature

The PbII binding characteristics of the previously reported PbII binding proteins of rat kidney cytosol were investigated further. Saturation and Scatchard analysis of "2"0"3Pb binding in whole cytosol and in 40% saturated ammonium sulfate precipitated fractions disclosed a class of relatively high-affinity sites with an apparent Kd of approximately 50 nM and binding capacities of approximately 41 and 9 pmol/mg of protein, respectively. Two "2"0"3Pb binding proteins with approximate molecular masses of 63K and 11.5K daltons and a high molecular weight component (greater than 200K) were isolated by Sepharose-6B column chromatography. The time course of association of "2"0"3Pb with cytosol and the 63K protein showed maximum binding at 18 hr which was stable up to 25 hr at 4 degrees C. The approximate half-time dissociation rate (T 1/2) of specifically bound "2"0"3Pb to the 63K protein ...

379

Favism, with special reference to Iran*

BackgroundAlterations in protein composition and oxidative damage of high-density lipoprotein (HDL) have been proposed to impair the cardioprotective properties of...Full Text Available

2010-07-04

380

Evaluation of the effect of Maytenus ilicifolia on the labelling of red blood cells and plasma proteins with Technetium-99m

Fava beans (Vicia fava) are cultivated rather widely in most countries of the Eastern Mediterranean area and provide a cheap but protein-rich food that can be eaten alone, in various...Full Text Available

1969-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Evaluating concentration estimation errors in ELISA microarray experiments

English 1998 p. 196 Brazil Oliveira, JF Braga, ACS Santos, JS Bernardo Filho,

1998-05-23

382

Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance

BackgroundEnzyme-linked immunosorbent assay (ELISA) is a standard immunoassay to estimate a protein's concentration in a sample. Deploying ELISA in a microarray format permits simultaneous...Full Text Available

383

British Library Electronic Table of Contents (United Kingdom)

Aims A depressed cGMP-dependent protein kinase (PKG) activity is implicated in nitrate tolerance. The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance. Methods and results Nitrate tolerance in in vitro preparations was obtained by a 24 h incubation with nitroglycerin (NTG). Nitrate tolerance in in vivo preparations was obtained by subcutaneous injection of mice with NTG, and the aortas were used. Protein levels of total MYPT1, MYPT1 (LZ+), PP1Cd, myosin light chain (MLC), and phosphorylated MLC were determined by Western blot analysis. Isometric vessel tension was determined by an organ chamber technique. Protein levels of MYPT1...

2010-01-01

384

Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi

Comparative study of functional properties of commercial and membrane processed yellow pea protein isolates

English 21 Jan 2001 [10 p.] United Kingdom Kumaran, D Eswaramoorthy,

2001-01-21

385

British Library Electronic Table of Contents (United Kingdom)

Functional properties of commercial and membrane processed pea protein isolates (PPI) prepared from yellow peas were investigated. Four protein isolates were prepared from yellow pea flour using water and KCl extractions at 25^oC followed by ultrafiltration and diafiltration (UF and DF) at pHs of 7.5 and 7.5 or 6 respectively. Following assessment of compositional attributes; solubility, foaming, flow and dynamic rheology, emulsification ability and heat-induced textural and rheological properties of prepared PPIs and a commercially available PPI were tested and compared. Membrane purification of proteins resulted in 28% to 68% reduction in phytic acid and enhanced, comparatively, the tested functional properties. Solubility of membrane processed PPIs, at all tested pHs, was superior and t...

2011-01-01

386

Clonidine, octopaminergic receptor agonist, reduces protein feeding in the blow fly, Phormia regina (Meigen)

British Library Electronic Table of Contents (United Kingdom)

Results in this study are consistent with those of Murdock and his colleagues who clearly demonstrated that clonidine, an agonist of octopaminergic receptors in some insects, significantly increases sucrose feeding. Their studies, however, did not examine the effect of clonidine on protein feeding. Injection of a 20mg/ml/fly dose of clonidine significantly reduces protein feeding in both sexes of Phormia regina, instead of stimulating feeding as is observed with carbohydrate feeding. The manner in which the flies are fed prior to starvation and the method of testing influences the amounts of diet consumed. It is proposed that the biogenic amines influence the state of hunger (i.e., protein versus carbohydrates) while other chemicals and neural mechanisms (i.e., such as sulfakinins and stre...

2007-01-01

387

Carbohydrate-specified endocytosis: localization of ligand in the lysosomal compartment.

BzpF is a CREB-like transcription factor that regulates spore maturation and stability in Dictyostelium

Carbohydrate-directed endocytosis is mediated by a receptor, the hepatic binding protein; it is responsible for the clearance of galactose-terminated glycoproteins from the circulation. This process...Full Text Available

1981-11-01

388

British Library Electronic Table of Contents (United Kingdom)

The cAMP response element-binding protein (CREB) is a highly conserved transcription factor that integrates signaling through the cAMP-dependent protein kinase A (PKA) in many eukaryotes. PKA plays a critical role in Dictyostelium development but no CREB homologue has been identified in this system. Here we show that Dictyostelium utilizes a CREB-like protein, BzpF, to integrate PKA signaling during late development. bzpF^- mutants produce compromised spores, which are extremely unstable and germination defective. Previously, we have found that BzpF binds the canonical CRE motif in vitro. In this paper, we determined the DNA binding specificity of BzpF using protein binding microarray (PBM) and showed that the motif with the highest specificity is a CRE-like sequence. BzpF is necessary to ...

2011-01-01

389

Avoidance of hydrolyzed casein by mice

Association and expression study of synapsin III and schizophrenia

When casein, a milk protein, is hydrolyzed, it renders human foods that contain it (e.g., hypoallergenic infant formula, cheeses) distasteful to many people. This rejection...Full Text Available

2008-01-28

390

Application of laser fluorimetry for determining the influence of a single amino-acid substitution on the individual photophysical parameters of a fluorescent form of a fluorescent protein mRFP1

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter...Full Text Available

2009-11-20

391

Alpha-toxin of Staphylococcus aureus.

Individual photophysical parameters of the chromophore of a fluorescent protein mRFP1 and its two mutants (amino-acid substitution at position 66 - mRFP1/ Q66C and mRFP1/Q66S proteins) are determined. For this purpose, apart from conventional methods of fluorimetry and spectrophotometry, nonlinear laser fluorimetry is used. It is shown that the individual extinction coefficients of the chromophore of proteins correlate (correlation coefficient above 0.9) with the volume of the substituted amino-acid residue at position 66 (similar to the positions of the absorption, fluorescence excitation and emission maxima). (laser biology and medicine)

2009-03-31

392

Adducted proteins for identification of endogenous electrophiles.

Alpha-toxin, the major cytotoxic agent elaborated by Staphylococcus aureus, was the first bacterial exotoxin to be identified as a pore former. The protein is secreted as a single-chain, water-soluble...Full Text Available

1991-12-01

393

AEC syndrome - Genetics Home Reference

Chemically reactive compounds in tissues can be monitored through their products of reaction with biomacromolecules. For the purpose of in vivo dose monitoring, hemoglobin (Hb) has been preferred to...Full Text Available

1993-03-01

394

A comprehensive assessment of N-terminal signal peptides prediction methods

What genes are related to AEC syndrome? AEC syndrome is caused by mutations in the TP63 gene. This gene provides instructions for making a protein known as p63, which plays an...

2011-10-15

395

0100127 - Liquid nitrogen dewar for protein crystal growth - NASA

BackgroundAmino-terminal signal peptides (SPs) are short regions that guide the targeting of secretory proteins to the correct subcellular compartments in the cell. They are cleaved...Full Text Available

396

The Coming Nuclear Renaissance for Next Generation Safeguards Specialists--Maximizing Potential and Minimizing the Risks

Gaseous Nitrogen Dewar apparatus developed by Dr. Alex McPherson of the University of California, Irvine for use aboard Mir and the International Space ...

397

Study of the Smith-Purcell effect in the relativistic regime

This document is intended to provide an overview of the workshop entitled 'The Coming Nuclear Renaissance for the Next Generation Safeguards Experts-Maximizing Benefits While Minimizing Proliferation Risks', conducted at Oak Ridge National Laboratory (ORNL) in partnership with the Y-12 National Security Complex (Y-12) and the Savannah River National Laboratory (SRNL). This document presents workshop objectives; lists the numerous participant universities and individuals, the nuclear nonproliferation lecture topics covered, and the facilities tours taken as part of the workshop; and discusses the university partnership sessions and proposed areas for collaboration between the universities and ORNL for 2009. Appendix A contains the agenda for the workshop; Appendix B lists the workshop attendees and presenters with contact information; Appendix C contains graphics of the evaluation form results and survey areas; and Appendix D summarizes the ...

2009-01-01

398

Report on the International Peer Review of RAINS-ASIA

We propose to investigate the spontaneous emission of radiation arising out of the interaction of a relativistic electron beam with a metallic grating (the Smith-Purcell effect). The work will concentrate, primarily, in the 50-120 #mu#m part of the infrared spectrum and will be an extension of the work begun by the Oxford-Dartmouth-Essex collaboration; one of the early objectives of the project will be to develop a quantitative understanding of the power spectrum of the emitted radiation over a wide range of emission angles. In particular, the limits of relativistic peaking of forward directed emission will be investigated. The electron beam will be produced by laser irradiation of a metallic cathode in the terminal of a small Van de Graff accelerator located in the Technische Universitaet, Muenchen. Beam energies will be in the range of 2 - 4 MeV. Initial tests on photoproduction of electrons have yielded 10 mA pulses with a width of about 20 ns. The electron ...

1995-08-21

399

Report on system operation - A background report prepared by the Nordel Operation Committee/OPG in the Nordel project on enhancing efficient functioning of the Nordic electricity market

Many Asian countries have experienced rapid economic growth during recent years and this trend is expected to continue. The economic growth is accompanied by increasing energy demand, with coal as the dominant energy source. For the first time a model has been specifically developed and applied for integrated assessment of future SO{sub 2} emissions in Asia. The report deals with the RAINS-ASIA model developed in Norway offering an opportunity to assess sulphur deposition and ecosystem protection levels resulting from different energy pathways and different emission control strategies. The cost of various control strategies are also provided. During the project an impressive database has been collected for the region (23 countries, 94 subregions and 355 large point sources). This was made possible through dedicated work by the project team, in close collaboration with scientific and technical institutions in several Asian countries. A further development of the ...

1995-10-01

400

Protocol to the 1994 Co-operation Agreement between The Government of the Islamic Republic of Pakistan and CERN concerning a Special Contribution by Pakistan towards the Construction of the LHC

This report contains the operation procedures in extreme situations and lessons learned from blackouts, based on a mandate given by the Operations Committee. Nordic TSOs have had common rules and principles for system operation i.e. System Operation Agreement since the late 1990s. The rules have been revised according to the system security and market changes and needs. The collaboration between system operators is regular and fruitful. The knowledge and capability to manage extreme situations of the Nordic power system has continuously increased and the recent blackouts did not show major deficiencies in operation practices. Regardless of the existing good collaboration it is important to continue development in the future in order to ensure high system security. Important development areas are security of reserve supervision and coordination of calculation procedures for transmission limits, enhancement of data exchange routines between ...

2004-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

New developments in plasma nitriding: Outcomes of AINSE collaborations

CERN signed a Co-operation Agreement with the Government of Pakistan in 1994, which was followed in 1997 by a Protocol signed with the Pakistan Atomic Energy Commission (PAEC) concerning CMS magnet supports. CERN and PAEC then signed in 1998 a Memorandum of Understanding for collaboration in the construction of the CMS detector. The participation by Pakistani scientists and engineers in the CERN programme is channelled through the National Centre of Physics (NCP) established at the Quaid-i-Azzam University in Islamabad, under the terms of a 1999 Protocol to the 1994 Co-operation Agreement. The engineering and detector construction work performed under these Agreements has been of high quality, and the Pakistani participation is valued highly by the CMS collaboration. In continuation of this co-operation, Pakistan, represented by PAEC as funding agency, has now proposed to make in-kind contributions to the LHC Project, such as mechanical ...

2003-01-01

402

Induced current switching development work for the application of ground interrupter switches on the Hydro-Quebec H.V. system

As a hardening process, the attractiveness of nitriding lies in its low treatment temperature. Although the thickness of the case is less than that obtained by higher temperature processes such as carburising, there are a range of alloys and applications for which nitriding is the only possible alternative. Indeed, there are some alloys and applications for which even the normal nitriding temperatures (between 500 deg C and 600 deg C for ferrous alloys) are too high. In this paper, we report on developments, specifically the use of low pressure plasmas and high energy ion bombardment, which can extend the benefits of nitriding to lower temperatures. In this poster, we will present results from these collaborative projects, concentrating on the nitriding of austenitic stainless steel at temperatures below 450 deg C. In this temperature range, the hardness and wear resistance of the surface can be improved without compromising the corrosion resistance of the ...

1998-12-02

403

Impact of the recent results by the CMS and ATLAS Collaborations at the CERN Large Hadron Collider on an effective Minimal Supersymmetric extension of the Standard Model

Phenomena associated with induced currents and voltages on high voltage transmission lines were discussed. Basic principles were reviewed. Action taken by Hydro-Quebec to assess the importance of associated problems on its grid was described. Hydro-Quebec`s findings and recommendations were compared with those of the International Standard, IEC 1129. It was concluded that for most cases, induced currents and voltages were higher than those listed under Class A recommendations of IEC 1129 as rated switching capabilities for ground switches assigned to this duty. For worst case scenarios, it was also found that, values of induced currents and voltages were lower than those of Class B recommendations. Hydro-Quebec and GEC Alsthom T and D collaborated to design and type test a full range of low cost and efficient ground interrupter switches tailored to Hydro-Quebec`s needs. A list of general application criteria of ground interrupter switches that evolved from that ...

1995-12-31

404

High-precision determination of the light-quark masses from realistic lattice QCD

We discuss the impact for light neutralinos in an effective Minimal Supersymmetric extension of the Standard Model of the recent results presented by the CMS and ATLAS Collaborations at the CERN Large Hadron Collider for a search of supersymmetry in proton-proton collisions at a center-of-mass energy of 7 TeV with an integrated luminosity of 35 inverse pb. We find that, in the specific case of light neutralinos, efficiencies for the specific signature searched by ATLAS (jets+missing transverse energy and an isolated lepton) imply a lower sensitivity compared to CMS (which searches for jets +missing transverse energy). Focusing on the CMS bound, if squark soft masses of the three families are assumed to be degenerate, the combination of the ensuing constraint on squark and gluino masses with the experimental limit on the b to s + gamma decay imply a lower bound on the neutralino mass that can reach the value of 11.9 GeV, depending on the gluino mass. On the other ...

2011-01-01

405

{ital J}/{ital {psi}} Suppression in Pb-Pb Collisions: A Hint of Quark-Gluon Plasma Production?

Three-flavor lattice QCD simulations and two-loop perturbation theory are used to make the most precise determination to date of the strange-, up-, and down-quark masses, $m_s$, $m_u$, and $m_d$, respectively. Perturbative matching is required in order to connect the lattice-regularized bare- quark masses to the masses as defined in the \\msbar scheme, and this is done here for the first time at next-to-next-to leading (or two-loop) order. The bare-quark masses required as input come from simulations by the MILC collaboration of a highly-efficient formalism (using so-called ``staggered'' quarks), with three flavors of light quarks in the Dirac sea; these simulations were previously analyzed in a joint study by the HPQCD and MILC collaborations, using degenerate $u$ and $d$ quarks, with masses as low as $m_s/8$, and two values of the lattice spacing, with chiral extrapolation/interpolation to the physical masses. With the new perturbation theory ...

2006-01-01

406

Zephyr: A secure Internet process to streamline engineering

The NA50 Collaboration has recently observed a strong suppression of {ital J}/{psi} production in Pb-Pb collisions at 158GeV/nucleon. We show that this recent observation finds a quantitative explanation in a model which relates the suppression mechanism to the local energy density, whose value is higher in Pb-Pb collisions than in any other system studied previously. The sensitivity of the phenomenon to small changes in the energy density could be suggestive of quark-gluon plasma formation. {copyright} {ital 1996 The American Physical Society.}

1996-08-01

407

Workshop on NN Bremsstrahlung and Polarization Observables

Lawrence Livermore National Laboratory (LLNL) is implementing an Internet-based process pilot called `Zephyr` to streamline engineering and commerce using the Internet. Major benefits have accrued by using Zephyr in facilitating industrial collaboration, speeding the engineering development cycle, reducing procurement time, and lowering overall costs. Programs at LLNL are potentializing the efficiencies introduced since implementing Zephyr. Zephyr`s pilot functionality is undergoing full integration with Business Systems, Finance, and Vendors to support major programs at the Laboratory.

1998-05-12

408

Web portals, instant messaging and web communities: new tools for online collaboration

The traveler participated in the discussions about the physics that can be learned in the experiments being planned, those presently running, or those recently completed by a growing number of major laboratories in Europe and the USA at the 'Workshop on NN Bremsstrahlung and Polarization Observables', where many representatives of these experimental programs were present. The traveler's research in this field is supported by a DOE Grant, and she has pioneered the early theoretical NN(lambda) treatments. In addition, she visited the Vrije University to collaborate with Professor Henk Blok on proton scattering from Zr-90 and neighboring nuclei. The traveler also visited NIKEF and discussed possible future experiments of mutual interest to be done at that laboratory.

1993-01-01

409

Travel Grant To Develop Us Collaboration On Contaminant Source Remediation Testing Facility

Web portals are nowadays very popular. This type of web sites let users to share information, request advice or help in a particular field, and furthermore allow them to create and extend sites content. Combined with instant messaging systems, used to send messages or files instantaneously to a user or a group of users, and with various kinds of chat programs, which connect two or more individuals simulating a conversation, it is now possible to create "web communities". In this paper we present the opensource tools used to create the "Grid Support Community" of the National Institute for Nuclear Physics in Italy.

2003-01-01

410

The nanoworld through aberration corrected lenses

ObjectivesObjectives Not AvailableDescriptionThe Cambridge University Engineering Department has granted the PI sabbatical leave in the academic year 2000/2001. Dr. T. Illangasekare from the Colorado School of Mines (CSM), USA has invited the PI to participate in his research during the period of December 2000 to September 2001. Recently, the Geotechnical and Environmental Geotechnics Group at Cambridge was successful in obtaining the Joint Infrastructure Fund to build a new laboratory/facilities to study geotechnical processes in constru [continued...

2001-01-31

411

The development and application of photonic technology in infrared and electro-optic countermeasures

The advent of aberration correction for electron microscope lenses has produced a significant advance in the improvement of resolution in microscopy. This improvement, while significant in itself, promises to have its most profound impact in materials science when it delivers quantitative information to challenge models and modellers. This capability for an electron microscope-modelling synergy to deliver useful results at the atomic level is not yet firmly established. For this reason, one of the major challenges for electron microscopy in materials science over this decade is to study systems where electron microscopists and modellers can work collaboratively. This paper explores some examples.

2010-07-01

412

Status of R and D of the plasma facing components for the ITER divertor

In this paper we discuss recent work at the Advanced Technology Centre of BAE Systems on photonic technology, in particular photonic crystal fibres, applied to infra-red and electro-optic countermeasure systems. The use of Photonic Crystal fibres or holey fibres in countermeasure systems could significantly simplify platform integration by enabling remote location of laser sources, the generation of multiple wavelengths or continuum generation from a single pump source .The paper will describe the development of these fibres, drawing examples from recent civil collaborative research projects such as PFIDEL and LAMPS.

2007-10-01

413

Star Formation in the Outer Disks of Spiral Galaxies

The paper reports the progress made by the ITER Home Teams in the development of robust carbon and tungsten armoured plasma facing components for the ITER divertor. The activities on the development and study of armour materials, joining technologies, non-destructive evaluation techniques, high heat flux testing of manufactured components and neutron irradiation resistance studies are presented. The results of these activities confirm the feasibility of the main divertor components. Examples of the fruitful collaboration between Parties and future R and D needs are also described. (author)

2001-05-01

414

Research, Preservation, and Education: An Introduction to Various Heritage Centers, Organizations, and Projects

This is a study done in collaboration with Deidre Hunter at Lowell Observatory studying star formation in two luminous spiral galaxies NGC 801 and UGC 2885. We used ultra-deep H? images taken at the KPNO 2.1 m telescope. We compare these data to stellar images at various wavelengths and to HI maps to determine the extent of star formation activity into the outer disk in these galaxies and its relationship to the gas and older stars. TW is grateful for an REU internship during the summer of 2010 at Northern Arizona University, funded by NSF through grant AST-1004107.

2011-01-01

415

British Library Electronic Table of Contents (United Kingdom)

This forum showcases the work of a variety of different heritage-based centers, organizations, and projects dedicated to research, education, and preservation of tangible and intangible forms of cultural heritage. The descriptions of these centers demonstrate the diversity of heritage work being done today. The centers and projects described in the forum vary in their contexts, missions, and outcomes. Highlighted in the forum are preservation organizations, university-based heritage centers, and a global collaborative cultural heritage project. Each organization in the forum provides information about their missions and goals, their approaches or methods to heritage work, and a brief description of some of their initiatives.

2011-01-01

416

Radiation and thermal analysis of superconducting quadrupoles in the interaction region of linear collider

Proton and deuteron spin structure function measurements in the resonance region

CERN has encouraged the US-LARP collaboration to participate in Phase I of the LHC luminosity upgrade by analyzing the benefits gained by using Nb3Sn technology to replace the functionality of select NbTi magnets that CERN is committed to construct. Early studies have shown that the much higher gradients (shorter magnetic lengths) and temperature margins (quench stability) of Nb3Sn magnets compared to their NbTi counterparts is favorable--allowing the insertion of additional absorbers between Q1 and Q2, for example. This paper discusses the relative merits of the NbTi and Nb3Sn options.

2008-06-01

417

Paul Scherrer Institute Scientific Report 2000. Volume IV: Nuclear Energy and Safety

The RSS collaboration has measured the spin structure functions of the proton and the deuteron at Jefferson Lab using the Hall C HMS spectrometer, a polarized electron beam and a polarized solid target. The asymmetries A and A were measured in the region of the nucleon resonances (0.82 GeV < W < 1.98 GeV) at an average four momentum transfer of Q2 = 1.3 GeV2. The extracted spin structure functions and their kinematic dependence will make a significant contribution in the study of higher-twist effects and polarized duality tests. A description of the experiment and the latest findings of the analysis will be presented.

2003-07-01

418

Osmoregulation in methanogens. Progress report, May 15, 1991--January 15, 1993

Nuclear energy related research in Switzerland is concentrated at PSI's Nuclear Energy and Safety Research Department (NES). The activities of the department are concentrated on three main domains of: Safety and related problems of operating plants; safety features of future reactor and fuel cycles; waste management. Comprehensive assessments of energy systems are carried out in cooperation with PSI's General Energy Research Department. Many of the programs are part of collaborations with universities, industry, or international organisations. Progress in 2000 in these topical areas is described in this report. A list of scientific publications in 2000 is also provided.

2001-03-01

419

Osmoregulation in methanogens

Our major goal of our work has been to develop and use NMR techniques to study how methanogenic archaebacteria deal with osmotic stress with the hope of providing insights into increasing the salt tolerance of other cells. The project has three main sections: (i) in vivo studies of methanogens; (ii) use of {sup l3}C- and {sup l5}N- labeled potential precursors and in vitro analyses of specific label uptake for elucidation of osmolyte dynamics and biosynthetic pathways of osmolytes in these organisms, and isolation of key biosynthetic enzymes; and (iii) collaborative studies on identification of organic solutes in other methanogens.

1993-01-01

420

Nutrient regulation of the saprotroph to parasite transition in Pochonia chlamydopsoria, a soil microbial inoculant for nematode control

Our major goal of our work has been to develop and use NMR techniques to study how methanogenic archaebacteria deal with osmotic stress with the hope of providing insights into increasing the salt tolerance of other cells. The project has three main sections: (i) in vivo studies of methanogens; (ii) use of [sup l3]C- and [sup l5]N- labeled potential precursors and in vitro analyses of specific label uptake for elucidation of osmolyte dynamics and biosynthetic pathways of osmolytes in these organisms, and isolation of key biosynthetic enzymes; and (iii) collaborative studies on identification of organic solutes in other methanogens.

1993-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Nuclear education in Russia: Status, peculiarities, problems and perspectives

DescriptionThe nematophagous fungus, Pochonia chlamydosporia is a facultative parasite that has been developed through collaborative research between Rothamsted and the Centro Nacional de Sanidad Agropecuaria, Havana, Cuba, as the biocontrol agent Klamic against root-knot nematodes in tropical soils. The abundance of this fungus in soil is not necessarily related to its effectiveness as a biological control agent and its parasitic activity against nematode eggs is related to its nutrition and physiological [continued...

2011-01-31

422

National Earth Comfort Program, Geothermal Heat Pump Market Mobilization and Technology Demonstration

The paper is devoted to analysis of Russian nuclear education system: its current status, specific features, difficulties and prospects. Russian higher education system in nuclear engineering has been created simultaneously with the development of nuclear industry, and the system completely satisfied all industrial demands for the specialists of different qualification levels. For the past several decades, nuclear education has lost its attractiveness to young people. The paper discusses the actions to be undertaken for reversing the situation. The paper underlines particularly the special role of international collaboration and all-European integration of nuclear educational programmes for further development of nuclear education all over the world. (author)

2005-07-01

423

Moral conflicts in project-based learning in ISD

Late 1994, the Geothermal Heat Pump Consortium (GHPC) was launched as a non-profit collaborative effort between the United States Department of Energy, US electric utilities and the GeoExchange (geothermal heat pump) industry to make ''geothermal heat pumps'' a significant component of the HVAC industry and avoid 1.5 million metric tons of carbon equivalent per year. The goal was to create a self-sustainable GeoExchange market. This report offers a summary of some of the lessons learned and an overview of some of the programs major accomplishments.

2001-04-30

424

British Library Electronic Table of Contents (United Kingdom)

Purpose - The purpose of this paper is to increase understanding of moral conflicts in information systems development by studying student perceptions in an information systems (IS) project course implemented in close collaboration with information technology firms. Design/methodology/approach - The paper is based on an empirical case study. Students' perceptions of moral conflicts were gathered through diaries, drawings, and questionnaires; and the analysis was inspired by phenomenography. Findings - The analysis reveals six types of moral conflicts. Students face conflicts related to their work tasks and to human and outside parties, and inherent in them is an inclination to moral failure in terms of doing something they perceive to be morally wrong, and also the desire for moral success...

2010-01-01

425

Midwest Superconductivity Consortium: 1994 Progress report

Meier associates and Pacific Northwest Laboratory staff exchange: Transfer of corrosion monitoring expertise to assess and develop in-line inspection tools for corrosion control

The mission of the Midwest Superconductivity Consortium, MISCON, is to advance the science and understanding of high {Tc} superconductivity. During the past year, 27 projects produced over 123 talks and 139 publications. Group activities and interactions involved 2 MISCON group meetings (held in August and January); with the second MISCON Workshop held in August; 13 external speakers; 79 collaborations (with universities, industry, Federal laboratories, and foreign research centers); and 48 exchanges of samples and/or measurements. Research achievements this past year focused on understanding the effects of processing phenomena on structure-property interrelationships and the fundamental nature of transport properties in high-temperature superconductors.

1995-01-01

426

Irradiation studies of fusion reactor materials utilizing FFTF/MOTA

Staff exchanges, such as the one described in this report, are intended to facilitate communication and collaboration among scientists and engineers at DOE laboratories, in US industry, and academia. During the past 5 years, PNL has developed prototype instrumentation to automate the data collection required for electrochemical determination of corrosion rates and behavior of materials in various electrically conductive environments. The last version is labeled the Sentry 100 prototype corrosion data scanner. Applications include these in the pulp and paper industry and at hazardous waste sites.

1995-04-01

427

Investigation of Various Wind Turbine Drivetrain Condition Monitoring Techniques

The most important and difficult part of materials research for fusion reactor is realized to be irradiation studies of fusion reactor materials. Irradiation studies of fusion reactor materials utilizing FFTF/MOTA, as one of Japan/U.S.A. Fusion Collaboration Programs, have important role to establish fundamental understanding of heavy irradiation effects on materials behavior and properties and to develop methods and technologies for advanced irradiation studies under fusion reactor environment. This paper briefly reviews the history, the state of the art, and the future of the FFTF/MOTA program. (author).

428

Intra-arterial injection of 131-I-Lipiodol in the treatment of hepato-cellular carcinoma; Lipiocis et carcinome hepato-cellulaire

The wind industry has experienced premature turbine component failures during the past years. With the increase in turbine size, these failures, especially those found in the major drivetrain components, i.e. main shaft, gearbox, and generator, have become extremely costly. Given that the gearbox is the most costly component in the drivetrain to fix, the National Renewable Energy Laboratory (NREL) initiated the Gearbox Reliability Collaborative (GRC) to determine the causes for premature gearbox failures and subsequently, recommend improvements to gearbox design, manufacture, and operational practices. The GRC has two identical test gearboxes, which are planned for a dynamometer and a field test, respectively.

2010-08-01

429

INSPIRE - The Next-Generation HEP Information System

The incidence of hepatocellular carcinoma (HCC) is increasing in developed countries and the general status of HCC patients is now by far better than a few years ago, allowing physicians to propose some different treatments of these patients. Among these treatments, intra-arterial injection of 131-iodine-labelled Lipiodol could be used in palliative, curative or adjuvant settings. After a brief summary of the modalities of this treatment, requiring the collaboration of Gastro-enterologists, Oncologists, Radiologists and Nuclear Medicine physicians, the different possibilities of therapeutic uses of this new approach are described and an outlook of these developments is proposed. (author)

2001-11-01

430

Final Technical Report on DOE Awards DE-FG03 94ER61918, DE-FG06 94ER61918 to Oregon Health Sciences University, September 15, 1994 - September 29, 1999

CERN, DESY, Fermilab and SLAC have joined forces to build INSPIRE, the next-generation HEP information platform offering innovative tools for information discovery and communication. Representing a natural community-based evolution of SPIRES, INSPIRE provides fast access to the entire body of HEP literature. As a subject repository it will host fulltexts of preprints, Open Access journal articles and supplementary material like conference slides and multimedia, enabling novel text- and data mining applications. In the spirit of Web2.0 INSPIRE will also supply tools for collaboration and user-enriched content.

2010-01-01

431

Connectivity in the Astronomy Digital Library

This report describes the activities conducted with DOE funds at Oregon Health Sciences University between 9/15/94 and 9/29/99. The activities fall into four major categories: Information Technology, Information Services and Support, Medical Informatics and Outcomes Research, and collaboration with other institutions. The focus of these activities was to implement and maintain a regional healthcare information network.

1999-11-23

432

Clean energy partnerships: A decade of success

The Astrophysics Data System (ADS) provides an extensive system of links between the literature and other on-line information. Recently, the journals of the American Astronomical Society (AAS) and a group of NASA data centers have collaborated to provide more links between on-line data obtained by space missions and the on-line journals. Authors can now specify which data sets they have used in their article. This information is used by the participants to provide the links between the literature and the data. The ADS is available at: http://ads.harvard.edu

2006-01-01

433

Chiral and Continuum Extrapolation of Partially-Quenched Hadron Masses

This report contains a partial catalog of recent accomplishments of the U.S. Department of Energy's Office of Energy Efficiency and Renewable Energy (EERE)in collaboration with its many private- and public-sector partners. This compendium of success stories illustrates the range and diversity of EERE programs and achievements. Part of an ongoing effort, the principal goal of this collection is to provide stakeholders with the evidence they need to assess the value they are receiving from investments in these DOE programs. The report begins with an introduction and a description of the methodology. It then presents an overview of the accomplishments of EERE programs. This is followed by the stories themselves.

2000-03-01

434

Centre for Quantum Computation & Communication Technology

Using the finite-range regularisation (FRR) of chiral effective field theory, the chiral extrapolation formula for the vector meson mass is derived for the case of partially-quenched QCD. We re-analyse the dynamical fermion QCD data for the vector meson mass from the CP-PACS collaboration. A global fit, including finite lattice spacing effects, of all 16 of their ensembles is performed. We study the FRR method together with a naive polynomial approach and find excellent agreement ~1% with the experimental value of M_rho from the former approach. These results are extended to the case of the nucleon mass.

2005-01-01

435

Breast imaging with a dedicated PEM

This is the homepage of "an Australian multi-university collaboration undertaking research on the fundamental physics and technology of building, at the atomic level, a solid state quantum computer in silicon together with other high potential implementations." Although attempts to develop a quantum computer have met with limited success, the centre has substantial resources invested in advancing toward practical uses of quantum computing technology. The site provides a very good introduction to the principles and implications of quantum computing, as well as details about various research projects underway at the Australian universities. Links to conference and journal papers produced by members of the centre, many from 2003, are also provided.

436

Aspects of unsteady aerodynamics in wind turbines

During the last decade there was a growing interest on the application of Positron Emission Tomography, PET, techniques to Breast Imaging. More recently, preliminary results suggested the use of dedicated devices to Breast Imaging using the same technique, the so-called Positron Emission Mammography cameras, PEM. In this article we review the arguments leading to a dedicated instrument. Based on these arguments we describe the concept of a PEM camera under development within the Crystal Clear Collaboration and the first results of its expected performance in terms of sensitivity and position resolution.

2004-07-11

437

Applications of X-ray scattering in pharmaceutical science

This presentation is primarily about unsteady aerodynamics in relation to wind turbines. It is divided into three very distinct sections: (1) Basic unsteady aerodynamics; (2) VAWT performance modelling and (3) Passive stall regulation of VAWTs. The aim of the presentation is to illustrate that, if desired, future wind turbine blades could be aerodynamically tailored to exploit the unsteady nature of the VAWT flow with far greater confidence and insight than has been hitherto possible. To this effect, the collaborating British research teams and, in particular, VAWT Ltd. have assembled sufficient knowledge for such a conclusion to be made. (author).

1990-01-01

438

British Library Electronic Table of Contents (United Kingdom)

The use of X-ray scattering techniques in pharmaceutical science is increasing, in part through increased collaborations with the materials science community, and through increased availability of instrumentation, particularly synchrotron sources. The ability to understand not only the biopharmaceutical outcome, but also arguably, more importantly, the structural aspects of drugs and drug delivery systems, is essential to progressing pharmaceutical science; this review serves as an introduction to the major techniques and the wide range of areas in which X-ray scattering may be applied in understanding and controlling structure in pharmaceutical systems.

2011-01-01

439

Antiproton-Proton Channels in J/psi Decays

An Explanation of the CDF Dijet Anomaly within a $U(1)_X$ Stueckelberg Extension

The recent measurements by the BES Collaboration of J/psi decays into a photon and a proton-antiproton pair indicate a strong enhancement at the proton-antiproton threshold not observed in the decays into a neutral pion and a proton-antiproton pair. Is this enhancement due to a proton-antiproton quasi-bound state or a baryonium? A natural explanation follows from a traditional model of proton-antiproton interactions based on G-parity transformation. The observed proton-antiproton structure is due to a strong attraction in the 1S0 state, and possibly to a near-threshold quasi-bound state in the 11S0 wave.

2005-01-01

440

Air Shower Events of High-Energy Cosmic Rays Measured at Seoul, South Korea

We discuss the recent excess seen by the CDF Collaboration in the dijet invariant mass distribution produced in association with a $W$ boson. We analyze the possibility of such a signal within the context of a $U(1)_X$ Stueckelberg extension of the Standard Model where the new gauge boson couples only to quarks. In addition to the analysis of the $Wjj$ anomaly we also discuss the production of $Zjj$ and $\\gamma jj$ at the Tevatron. The analysis is then extended to the Large Hadron Collider with $\\sqrt{s}=7 {\\rm TeV}$ and predictions for the dijet signals are made.

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

AONE Launches New Executive Coaching Resource for Members

The COsmic ray Research and Education Array (COREA) collaboration has installed an array of six detector stations at two high schools in and near Seoul, Korea for measurement of air-shower events from high-energy cosmic rays. Three stations are installed at each site, where each station consists of four plastic scintillation detectors covering an area of 2m2. In this presentation, we report the currenst status of the COREA project, describing the experimental equipment and measurement of coincident events.

2011-09-01

442

British Library Electronic Table of Contents (United Kingdom)

The American Organization of Nurse Executives (AONE) and The Leadership Studio have collaborated to create a one-of-a-kind Executive Coaching Resource Center for nurse leaders and their teams. This resource-designed specifically for AONE members-is a one-stop web-based library developed to assist nurse leaders who want to learn more about utilizing executive coaching services. It focuses on the overall benefits of coaching for nurse leaders-both tangible and intangible-and provides return on investment information for executive coaching based on the most current research.

2009-01-01

443

A preliminary account of wind speed data in the coastal regions of Bangladesh

The main activities and scientific collaboration possibilities at Ankara Nuclear research and training center

A wind energy study (WEST) in the coastal region of Bangladesh has been undertaken, in collaboration with Local Government Engineering Department (LGED) and with technical support of the UK`s Energy Technology Support Unit (ETSU) under an ODA programme. Seven 25m high masts have just been installed at selected places and data for a complete year will be recorded. A brief account of the mast erection is given and some preliminary data is presented. Results indicate that fresh data is going to give higher figures than the meteorological ones. (author)

1997-06-01

444

Study of the mass of the electron neutrino in Japan

Full text: Founded in 1964, Ankara Nuclear Research and Training Center (ANRTC) conducts and facilitates the scientific activities including training (summer practice, MSc and Ph D studies in physics and chemistry, IAEA fellowship programs etc.), research and other studies in nuclear and related fields. As it's a part of main duties, ANRTC has analysis on the variety of samples, and radiation protection services commercially, for radiation workers in state, public and private sectors. Research, development and application projects implemented in this Center have mostly been supported by State Planning Organization (SPO) and Turkish Atomic Energy Authority (TAEA). In addition to the projects there are on going collaborative studies with some national Universities and International Atomic Energy Agency. The main activities carried out in ANRTC can be summarized as: studies on experimental nuclear physics, application of nuclear techniques such as XRF, XRD, Gamma, ...

2004-10-01

445

Korea-Japan Joint Research on Development of Seismic Capacity Evaluation and Enhancement Technology Considering Near-Fault Effect (Final Report)

This report describes a study of the mass of the electron neutrino using electron capture in {sup 163}Ho in Japan for the period from 1981 to 1994. This monograph has two purposes, one is to supplement the papers on the mass of the electron neutrino already published by us and another is to make a record on some details of our experiments for future. Electron capture in a nucleus takes place in a rather small space inside an atom, where atomic physics, nuclear physics and particle physics work closely together. Therefore, this study needed an intimate collaboration of atomic physicists, nuclear physicists and particle physicists. In addition, it was necessary for this study to use various fine techniques, including metallurgy, production of {sup 163}Ho activity, micro-analysis by wet chemistry, isotope-dilution mass spectrometry, undulator radiation source technology, the soft X-ray monochromator technology, a counting technique for very intense soft X-rays and so ...

1996-02-01

446

Collaborative and situated learning on the web ? how can teacher education theoretically and practically respond to changing demands and roles of teachers? : A Research based on case studies of online second language learning in ?communities of practice?

We compiled the results of the source analysis obtained under the collaboration research. Recent construction scheme for source modeling adopted in Japan is described, and strong-motion prediction is performed assuming the scenario earthquakes occurring in the Ulsan fault system, Korea. Finally Qs values beneath the Korean inland crust are estimated using strong-motion records in Korea from the 2005 Off West Fukuoka earthquake (M7.0). Probabilistic seismic hazard for four NPP sites in Korea are evaluated, in which the site specific attenuation equations with Index SA developed for NPP sites are adopted. Furthermore, the uniform hazard spectra for the four NPP sites in Korea are obtained by conducting the PSHA by using the attenuation equations with the index of response spectra and seismic source model cases with maximum weights. The supporting tools for seismic response analysis, the evaluation tool for evaluating annual probability of failure, and system analysis ...

2006-12-15

447

Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits.

The background for this paper is a growing interest during the past decade in Denmark in the concepts of ?situated learning?, ?social theory of learning? and ?learning in communities of practice? developed by the American social anthropologist Jean Lave and the Californian Research Scientist Etienne Wenger. The work by Jean Lave from 1988 (Lave, 1988) based on anthropological field studies in Brazil and Liberia as well as later works by Lave & Wenger (Lave and Wenger, 1991; Chaiklin and Lave, 1993; Wenger, 1998; Wenger, McDermott, R. and W. Snyder, 2002) on situated learning and social learning theory have provoked considerable response in anthropological studies, in learning theories as well asin considerations about practical implementation of concepts like ?lifelong learning? in Denmark in recent decades. The European concept of ?lifelong learning? has had a big impact on educational thinking, including teacher education. In addition to this, IT has been playing a continuously ...

2006-01-01

448

Uteroglobin gene expression in the rabbit uterus throughout gestation and in the fetal lung. Relationship between uteroglobin and eicosanoid levels in the developing fetal lung.

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the...Full Text Available

1993-05-01

449

The molybdenum iron-sulphur protein from Desulfovibrio gigas as a form of aldehyde oxidase.

Uteroglobin (UG) gene encodes a cytokine-like, multifunctional, antiinflammatory protein, with potent phospholipase A2-inhibitory activity. It has been suggested that during implantation this protein...Full Text Available

1995-07-01

450

The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.

The molybdenum iron-sulphur protein originally isolated from Desulfovibrio gigas by Moura, Xavier, Bruschi, Le Gall, Hall & Cammack [(1976) Biochem. Biophys. Res. Commun. 72, 782-789] has been...Full Text Available

1987-05-01

451

The holographic principle and the language of genes

We have isolated and sequenced cDNA clones encoding the human U1-70K snRNP protein, and have mapped this locus (U1AP1) to human chromosome 19. The gene produces two size classes of RNA, a major 1.7-kb...Full Text Available

1987-12-23

452

The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin.

We show that the holographic principle in quantum gravity imposes a strong constraint on life. The degrees of freedom of an organism can be estimated according to the theory of Boolean networks, which is constrained by the entropy bound. Hence we can explain the languages in protein sequences or in DNA sequences. The overall evolution of biological complexity can be illustrated. And some general properties of protein length distributions can be explained by a linguistic mechanism.

2008-01-01

453

The Membrane Bound LRR Lipoprotein Slr, and the Cell Wall-Anchored M1 Protein from Streptococcus pyogenes Both Interact with Type I Collagen

The products of trithorax group (trxG) genes maintain active transcription of many important developmental regulatory genes, including homeotic genes. Several trxG proteins have been shown to act in...Full Text Available

2004-07-01

454

The Function of Hypoxia-Inducible Factor (HIF) Is Independent of the Endoplasmic Reticulum Protein OS-9

Streptococcus pyogenes is an important human pathogen and surface structures allow it to adhere to, colonize and invade the human host. Proteins containing leucine rich repeats (LRR)...Full Text Available

455

Temporal patterns of protein phosphorylation after angiotensin II, A23187 and/or 12-O-tetradecanoylphorbol 13-acetate in adrenal glomerulosa cells.

The protein “amplified in osteosarcoma-9” (OS-9) has been shown previously to interact with the prolyl hydroxylases PHD2 and PHD3. These enzymes initiate oxygen-dependent degradation...Full Text Available

456

Synthesis and cloning of the genes of antisense peptides of human calcitonin and miniproinsulin

The temporal patterns of protein phosphorylation in the adrenal glomerulosa cell were analysed by two-dimensional electrophoresis after stimulation with 10 nM-angiotensin II or various agents [10 nM-12-O-tetradecanoylphorbol...Full Text Available

1986-09-15

457

Structural features underlying selective inhibition of protein kinase CK2 by ATP site-directed tetrabromo-2-benzotriazole

With the aim of an experimental check on the validity of the theory of molecular recognition, the authors have carried out the chemical-enzymatic synthesis and cloning of the gene of human calcitonin and also of the genes of antisense polypeptides to human calcitonin and miniproinsulin. It has been shown that recombinant plasmids obtained on the basis of these synthetic genes are capable of ensuring the biosynthesis of the given polypeptides in E. coli cells as hybrid proteins with the IgG-binding domain of staphylococcal protein A.

1994-07-20

458

Structural Chemistry of Human SET Domain Protein Methyltransferases

Two novel crystal structures of Zea mays protein kinase CK2α catalytic subunit, one in complex with the specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) and another...Full Text Available

2001-11-01

459

Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)

There are about fifty SET domain protein methyltransferases (PMTs) in the human genome, that transfer a methyl group from S-adenosyl-L-methionine (SAM) to substrate lysines on histone tails or other peptides. A number of structures in complex with cofactor, substrate, or inhibitors revealed the mechanisms of substrate recognition, methylation state specificity, and chemical inhibition. Based on these structures, we review the structural chemistry of SET domain PMTs, and propose general concepts towards the development of selective inhibitors.

2011-08-22

460

SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

BackgroundLand plant genomes contain multiple members of a eukaryote-specific gene family encoding proteins with pentatricopeptide repeat (PPR) motifs. Some PPR proteins were shown...Full Text Available

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461

Roles of the TNF receptor-associated factor (TRAF)2/3 binding site in differential B cell signaling by CD40 and its viral oncogenic mimic, latent membrane protein 1 (LMP1)

We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be...Full Text Available

1990-05-01

462

Relative Rates of Transcapillary Movement of Free Thyroxine, Protein-Bound Thyroxine, Thyroxine-Binding Proteins, and Albumin

The Epstein-Barr virus protein, LMP1, is a functional mimic of the cellular receptor CD40, but signals to B lymphocytes in an amplified and sustained manner compared to CD40. LMP1 contributes...Full Text Available

2009-09-01

463

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation¹^[W]^[OA]

The rate of appearance of labeled thyroxine (T4) and albumin in lymph from various areas after simultaneous i.v. injection of the labeled substances in conscious ambulatory sheep has been used to estimate...Full Text Available

1974-07-01

464

RecA protein acts at the initiation of stable DNA replication in rnh mutants of Escherichia coli K-12.

Rice (Oryza sativa) seed storage proteins (SSPs) are synthesized and deposited in storage organelles in the endosperm during seed maturation as a nitrogen source for germinating seedlings....Full Text Available

2010-12-01

465

Protein oxidation markers in women with and without gestational diabetes mellitus: A possible relation with paraoxonase activity.

Escherichia coli rnh mutants lacking RNase H activity are capable of recA+-dependent DNA replication in the absence of concomitant protein synthesis (stable DNA replication). In rnh dnaA::Tn10 and rnh...Full Text Available

1985-08-01

466

Protein dynamics. Vibrational coupling, spectral broadening mechanisms, and anharmonicity effects in carbonmonoxy heme proteins studied by the temperature dependence of the Soret band lineshape.

AIMS: To clarify the levels of protein oxidation markers such as protein carbonyl (PCO), protein hydroperoxides (P-OOH), advanced oxidation protein products (AOPP) and nitrotyrosine (NT), as well as antioxidative enzymes such as paraoxonase (PON-1) in women with and without gestational diabetes mellitus (GDM). METHODS: The study was conducted on 23 women with GDM and 22 women without GDM. The levels of the P-OOH, AOPP, and PON-1 were determined by colorimetric methods; whereas NT and PCO levels were measured by ELISA. RESULTS: The concentrations of protein oxidation markers were significantly increased and PON1 activity was significantly decreased in GDM group compared to those of normal pregnant women. The control group showed a significant negative correlation between PON-1 and PCO (r=-0.451, p=0.027); whereas in GDM group, there was a significant positive correlation between ...

2011-08-30

467

Primary structure of streptococcal Pep M5 protein: Absence of extensive sequence repeats

In this work we study the temperature dependence of the Soret band lineshape of the carbonmonoxy derivatives of sperm whale myoglobin, human hemoglobin, and its isolated alpha and beta subunits. To...Full Text Available

1992-08-01

468

Position-Dependent Electrostatic Protection against Protein Aggregation

Extensive sequence repeats have been observed in a biologically active fragment of type 24 streptococcal M protein, namely Pep M24 [Beachey, E. H., Sayer, J. M. & Kang, A. H. (1978) Proc....Full Text Available

1983-09-01

469

British Library Electronic Table of Contents (United Kingdom)

Proteins with a high propensity to aggregate can be largely prevented from doing so with surprisingly small changes to their primary structure. By using a combination of rational design and quantitative measurements of aggregation rates, we show that adding a single charge in specific "gatekeeper" regions is sufficient to change the timescale for amyloid fibril growth from minutes to weeks, thereby dramatically reducing the efficiency of this process.

2009-01-01

470

Outcome predictability of biomarkers of protein-energy wasting and inflammation in moderate and advanced chronic kidney disease¹²³⁴

On the Origins of the Weak Folding Cooperativity of a Designed ??? Ultrafast Protein FSD-1

Background: Markers of protein-energy wasting (PEW) and inflammation are common in chronic kidney disease (CKD) and are among the strongest predictors of mortality in dialysis patients.Objective:...Full Text Available

2009-08-01

471

FSD-1, a designed small ultrafast folder with a ββα fold, has been actively studied in the last few years as a model system for studying protein folding mechanisms and for testing...Full Text Available

2010-11-01

472

Mutations Affecting Motifs of Unknown Function in the Central Domain of Nitrogen Regulatory Protein C

BackgroundUsing a transcriptional profiling approach, we recently identified myeloid-related protein-8/14 (MRP-8/14) to be expressed by platelets during acute MI....Full Text Available

2008-01-01

473

Mice With a Deletion in the Gene for CCAAT/Enhancer-Binding Protein ? are Protected Against Diet-Induced Obesity

The positive control function of the bacterial enhancer-binding protein NtrC resides in its central domain, which is highly conserved among activators of ς⁵⁴ holoenzyme. Previous...Full Text Available

1999-09-01

474

Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.

The CCAAT/enhancer-binding protein β (C/EBPβ) is required for adipocyte differentiation and maturation. We have studied the role of the transcription factor, C/EBPβ,...Full Text Available

2007-01-01

475

Involvement of stress-activated protein kinase and p38 mitogen-activated protein kinase in mIgM-induced apoptosis of human B lymphocytes

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation...Full Text Available

1996-12-02

476

Immunohistochemical localization of low density lipoprotein receptor-related protein 1 and ?₂-Macroglobulin in retinal and choroidal tissue of proliferative retinopathies

Despite intensive efforts, the intracellular signaling pathways that mediate apoptosis remain unclear. The human B lymphoma cell line, B104, possesses characteristics that make it an attractive model...Full Text Available

1996-11-26

477

Hyperphosphorylation of the Rotavirus NSP5 Protein Is Independent of Serine 67 or NSP2, and the Intrinsic Insolubility of NSP5 Is Regulated by Cellular Phosphatases

The immunolocalization of the low density lipoprotein receptor-related protein 1 (LRP1) and its ligand alpha 2-Macroglobulin (α₂M) was examined in tissues from human donor...Full Text Available

2010-08-01

478

HupUV proteins of Rhodobacter capsulatus can bind H2: evidence from the H-D exchange reaction.

The NSP5 protein is required for viroplasm formation during rotavirus infection and is hyperphosphorylated into 32- to 35-kDa isoforms. Earlier studies reported that NSP5 is not hyperphosphorylated...Full Text Available

2006-02-01

479

High throughput protein fold identification by using experimental constraints derived from intramolecular cross-links and mass spectrometry

The H-D exchange reaction has been measured with the D2-H2O system, for Rhodobacter capsulatus JP91, which lacks the hupSL-encoded hydrogenase, and R. capsulatus BSE16, which lacks the HupUV proteins....Full Text Available

1997-01-01

480

Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase ?

We have used intramolecular cross-linking, MS, and sequence threading to rapidly identify the fold of a model protein, bovine basic fibroblast growth factor (FGF)-2. Its tertiary structure was probed...Full Text Available

2000-05-23

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481

Helper T-Cell Epitopes Encoded by the Babesia bigemina rap-1 Gene Family in the Constant and Variant Domains Are Conserved among Parasite Strains

Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), the viral RNA-dependent RNA polymerase (RdRp), is a tail-anchored protein with a highly conserved C-terminal transmembrane domain (TMD) that...Full Text Available

2010-11-01

482

External reflection absorption infrared spectroscopy study of lung surfactant proteins SP-B and SP-C in phospholipid monolayers at the air/water interface.

Among important candidates for babesial vaccines are apical complex proteins, including rhoptry-associated protein 1 (RAP-1) from Babesia bovis and B. bigemina, which...Full Text Available

1998-04-01

483

Exploiting the beneficial effects of PPO on the utilisation of protein and lipids in grazed forages

The interactions of the hydrophobic pulmonary surfactant proteins SP-B and SP-C with 1,2-dipalmitoylphosphatidylcholine in mixed, spread monolayer films have been studied in situ at the air/water interface...Full Text Available

1995-12-01

484

Evaporator condensates: continuous bioprocessing of simulated sulfite condensates to produce single cell protein. [Candida utilis

DescriptionThe plant enzyme polyphenol oxidase (PPO) has been demonstrated to reduce both proteolysis and lipolysis during the ensiling of red clover. The purpose of this work is to explore the potential for exploiting this beneficial trait in other dietary regimes for grazing ruminants. These studies will focus on the development of new strategies for forage management and livestock feeding by increasing the efficiency of feed protein-N utilisation and reducing saturated fatty acid production by ruminan [continued...

2007-01-31

485

Ebola Virus VP40-Induced Particle Formation and Association with the Lipid Bilayer

The continuous bioprocessing of simulated steam-stripped sulfite pulp mill evaporator condensates using Candida utilis to produce single cell protein has been studied. At 32 degrees C, processing can be conducted at space velocities of up to 0.43 hr/sup -1/ with over 90% removal of acetic acid present at an initial concentration of 2 g/liter. (Refs. 16).

1982-11-01

486

Differential Changes in Heat Shock Protein-, Lipoarabinomannan-, and Purified Protein Derivative-Specific Immunoglobulin G1 and G2 Isotype Responses during Bovine Mycobacterium avium subsp. paratuberculosis Infection

Viral protein 40 (VP40) of Ebola virus appears equivalent to matrix proteins of other viruses, yet little is known about its role in the viral life cycle. To elucidate the functions of VP40, we investigated...Full Text Available

2001-06-01

487

Determination by enzyme-linked immunosorbent assay of immunoglobulin G (IgG), IgM, and IgA to Brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis.

Bovine paratuberculosis is caused by infection of young calves with Mycobacterium avium subsp. paratuberculosis. In some of the chronically infected cows the long asymptomatic...Full Text Available

2001-03-01

488

Decreased serum protein binding of diazepam and its major metabolite in the neonate during the first postnatal week relate to increased free fatty acid levels.

An enzyme-linked immunosorbent assay was used to compare Brucella melitensis major outer membrane proteins (MOMP) and whole-cell heat-killed antigens (HK) in measuring antibrucella immunoglobulin G...Full Text Available

1989-08-01

489

Decreased binding of drugs and dyes to plasma proteins from rats with acute renal failure: effects of ureter ligation and intramuscular injection of glycerol.

The protein binding of diazepam (D) and its major active metabolite N-desmethyl diazepam (DD) was investigated in vitro in the serum of 14 mothers at birth, 21 foetuses at birth, in 100 neonates between...Full Text Available

1984-01-01

490

1 The decreased binding of drugs and dyes to plasma proteins from male and female rats with acute renal failure has been investigated using equilibrium dialysis at 37 degrees C. 2 Acute renal failure...Full Text Available

1979-06-01

491

DNA repair genes

DIMINISHED DEGRADATION OF MYELIN BASIC PROTEIN BY ANTI-SULFATIDE ANTIBODY AND INTERFERON-? IN MYELIN FROM GLIA MATURATION FACTOR-DEFICIENT MICE

Fission yeast S. pombe is assumed to be a good model for cloning of human DNA repair genes, because human gene is normally expressed in S. pombe and has a very similar protein sequence to yeast protein. We have tried to elucidate the DNA repair mechanisms of S. pombe as a model system for those of mammals. (J.P.N.)

1995-12-01

492

Characterization of the major phosphoprotein and its kinase on the surface of the rat adipocyte

In this study we show the effect of anti-sulfatide (RmAb) antibodies and inflammatory cytokines, TNF-α and IFN-γ in inducing myelin basic protein (MBP) degradation in myelin...Full Text Available

2007-06-01

493

Binding the Mammalian High Mobility Group Protein AT-hook 2 to AT-Rich Deoxyoligonucleotides: Enthalpy-Entropy Compensation

Intact rat fat cell exposed to 12.5 ..mu..M (..gamma..-32P)ATP incorporate label into specific proteins within minutes. By solubilizing the reaction mixture with SDS which bypasses the subcellular fractionation steps, the labeled proteins can be identified in autoradiographs of SDS-PAGE gels. The most prominently labeled protein has an M/sub r/ of 42,000. Localization of this component to the cell surface can be made on the basis of inhibition of phosphorylation by addition of a protein derived from the rat brain with protein kinase inhibitory property, susceptibility of the phosphorylated protein to the tryptic digestion, inhibition of phosphorylation of this protein after brief exposure to melittin. To rule out the possibility that the cell surface protein might be a mitochondrial contaminant from broken cells, /sup ...

1986-12-01

494

Association between glucokinase regulatory protein (GCKR) and apolipoprotein A5 (APOA5) gene polymorphisms and triacylglycerol concentrations in fasting, postprandial, and fenofibrate-treated states¹²³

HMGA2 is a DNA minor-groove binding protein. We previously demonstrated that HMGA2 binds to AT-rich DNA with very high binding affinity where the binding of HMGA2 to poly(dA-dT)₂ is enthalpy-driven...Full Text Available

2009-05-20

495

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.

Background: Hypertriglyceridemia is a risk factor for cardiovascular disease. Variation in the apolipoprotein A5 (APOA5) and glucokinase regulatory protein (GCKR)...Full Text Available

2009-01-01

496

Amino acid sequences that determine the nuclear localization of yeast histone 2B.

Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two...Full Text Available

1991-11-01

497

Affinity labelling of ribosomes from the livers of different vertebrates by 2-nitro-4-azidobenzoyl-Phe-tRNA

Histone-beta-galactosidase protein fusions were used to identify the domain of yeast histone 2B, which targets this protein to the nucleus. Amino acids 28 to 33 in H2B were required for nuclear localization...Full Text Available

1987-11-01

498

5'-Azido-[3,6-3H2]-1-napthylphthalamic acid, a photoactivatable probe for naphthylphthalamic acid receptor proteins from higher plants: identification of a 23-kDa protein from maize coleoptile plasma membranes.

Ribosomal protein L 10 from the livers of trout, hen, and rat was found to be the main target for 2-nitro-4-azidobenzoyl-Phe-tRNA in affinity labelling experiments. Therefore, despite somewhat different electrophoretic mobilities, this protein seems to be involved in the organization of the peptidyl transferase centre in ribosomes of various vertebrates. (author).

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