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BackgroundDinoflagellates are unicellular, often photosynthetic protists that play a major role in the dynamics of the Earth's oceans and climate. Sequencing of dinoflagellate nuclear...Full Text Available

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Objectives. The purposes of this study were to test the hypothesis that vaginal douching is linked to bacterial vaginosis in both symptomatic and asymptomatic women and to identify...Full Text Available

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PMID:12439520

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PMID:10923545

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Bacterial cellulose was produced by Acetobacter xylinum (strain TISTR 975). Bacterial cellulose is an interesting material for using as a wound dressing since it provides moist environment to a wound resulting in a better wound healing. However, bacterial cellulose itself has no antimicrobial activity to prevent wound infection. To achieve antimicrobial activity, silver nanoparticles were impregnated into bacterial cellulose by immersing bacterial cellulose in silver nitrate solution. Sodium borohydride was then used to reduce the absorbed silver ion (Ag+) inside of bacterial cellulose to the metallic silver nanoparticles (Ag0). Silver nanoparticles displayed the optical absorption band around 420nm. The red-shift and broadening of the optical absorption band was observed when the mole rat...

British Library Electronic Table of Contents (United Kingdom)

Abstract Toll-like receptor 2 (TLR2) is an immune sensor for gram-positive bacterial cell wall components. Single-nucleotide polymorphisms (SNPs) in the TLR2 gene that impair its function may, therefore, influence the risk and outcomes of gram-positive bacterial infections. In a cohort of 694 liver transplant recipients, we assessed the TLR2 SNP that is translated into an amino acid substitution of arginine for glutamine at position 753 (R753Q), and we found that its presence was associated with the clinical characteristics and outcomes of gram-positive bacterial infections. The proportions of patients with the TLR2 R753Q SNP did not significantly differ between those with gram-positive bacterial infections and those without gram-positive bacterial infections (9.6% versus 9.6%, P = 0.999)....

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Mar 13, 1997 ... Rich Baldwin's data and program files from the workstation Geomag extracted 5/96 FILE DESCRIPTION /BINNER/ /u/baldwin/rich/fortran/binner/ ...

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Norepinephrine stimulates the growth of a range of bacterial species in nutritionally poor SAPI minimal salts medium containing 30% serum. Addition of size-fractionated serum components to SAPI...Full Text Available

UK PubMed Central (United Kingdom)

Fatty acid biomarker analysis coupled with gas chromatography-isotope ratio mass spectrometry was used to confirm the presence of methanotrophic and thiotrophic bacterial endosymbionts in the tissues...Full Text Available

UK PubMed Central (United Kingdom)

The results of antibiotic tests on bacterial pathogens freshly isolated from avian tissues and bovine milk are presented. Coliform isolates from the avian species showed an increased resistance to...Full Text Available

UK PubMed Central (United Kingdom)

The objective of this study was to determine if inflammatory tolerance and enhancement of innate immune function could be induced by the gram-positive cell wall component peptidoglycan (PGN)....Full Text Available

UK PubMed Central (United Kingdom)

Severe pericardial effusion is a rare complication of bacterial pneumonia and it usually disappears under medical treatment. Herein we report a case of a girl with a congenital immunodeficient syndrome...Full Text Available

UK PubMed Central (United Kingdom)

Neonatal bacterial infection in rats alters the responses to a variety of subsequent challenges later in life. Here we explored the effects of neonatal bacterial infection on a subsequent drug...Full Text Available

UK PubMed Central (United Kingdom)

Infection due to multidrug resistance pathogens is difficult to manage due to bacterial virulence factors and because of a relatively limited choice of antimicrobial agents. Thus, it is imperative to...Full Text Available

UK PubMed Central (United Kingdom)

Enteric infections with attaching/effacing lesion-inducing bacterial pathogens are a worldwide health problem. A murine infection model with one such pathogen, Citrobacter rodentium,...Full Text Available

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ObjectiveTo examine the role of first trimester bacterial vaginosis (BV) and level of BV-associated microorganisms diagnosed using the Nugent’s Gram stain...Full Text Available

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In the Archaea only a handful of ribonucleases involved in RNA processing and degradation have been characterized. One potential group of archaeal ribonucleases are homologues of the bacterial RNase...Full Text Available

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BackgroundThe bacterial genus Listeria contains pathogenic and non-pathogenic species, including the pathogens L. monocytogenes and L. ivanovii,...Full Text Available

UK PubMed Central (United Kingdom)

The purpose of the work was to quantitatively characterize temperature effects on the bacterial leaching of sulfide ore material containing several sulfide minerals. The leaching was tested at eight...Full Text Available

UK PubMed Central (United Kingdom)

We examined the bacterial aerobic nasal flora of 216 healthy volunteers to identify potential competitive interactions among different species, with special emphasis on the influence of staphylococcal...Full Text Available

UK PubMed Central (United Kingdom)

ObjectivesTo assess whether bacterial vaginosis or chlamydial infection before 10 weeks' gestation is associated with miscarriage before 16 weeks.DesignProspective...Full Text Available

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To diagnose asymptomatic bacterial vaginosis (BV), self-sampled vaginal smears were collected during a study of risk factors for preterm birth in African American women. More than 90% of those...Full Text Available

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... VDPEAZ (Bonn 2010 issue) NUCLEAR PHYSICS AND RADIATION PHYSICS

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Short communication.

Science.gov (United States)

The real-time polymerase chain reaction (PCR) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (BV) and candidiasis (CA) was performed. Statistical analysis revealed that the BV condition is characterised by a great variability among subjects and that it is associated with a significant increase of Prevotella, Atopobium, Veillonella and Gardnerella vaginalis, and a drop in Lactobacillus. On the contrary, the vaginal microflora of healthy women and patients developing CA was found to be homogeneous and stable over time. PMID:18762999

Science.gov (United States)

The emergence of bacterial antibiotic resistance is a growing problem, yet the variables that influence the rate of emergence of resistance are not well understood. In a microfluidic device designed to mimic naturally occurring bacterial niches, resistance of Escherichia coli to the antibiotic ciprofloxacin developed within 10 hours. Resistance emerged with as few as 100 bacteria in the initial inoculation. Whole-genome sequencing of the resistant organisms revealed that four functional single-nucleotide polymorphisms attained fixation. Knowledge about the rapid emergence of antibiotic resistance in the heterogeneous conditions within the mammalian body may be helpful in understanding the emergence of drug resistance during cancer chemotherapy. PMID:21940899

UK PubMed Central (United Kingdom)

Legionella pneumophila is a gram-negative bacterial species that is ubiquitous in almost any aqueous environment. It is the agent of Legionnaires’ disease, an acute and often...Full Text Available

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could be attributed either to removal of bacterial competition. (the reason for adding antibiotics before adding additional carbon and ...

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Periodontitis is an infectious process characterized by inflammation affecting the supporting structures of the teeth. Porphyromonas gingivalis is a major oral bacterial species implicated...Full Text Available

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Examining the relationship between biodiversity and functional stability (resistance and resilience) of activated sludge bacterial communities following disturbance is an important first step towards developing strategies for the design of robust biological wastewater treatment systems. This study investigates the relationship between functional resistance and biodiversity of dominant bacterial taxa by subjecting activated sludge samples, with different levels of biodiversity, to toxic shock loading with cupric sulfate (Cu[II]), 3,5-dichlorophenol (3,5-DCP), or 4-nitrophenol (4-NP). Respirometric batch experiments were performed to determine the functional resistance of activated sludge bacterial community to the three toxicants. Functional resistance was estimated as the 30?min IC50 or th...

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BackgroundThe metagenomic analysis of microbial communities holds the potential to improve our understanding of the role of microbes in clinical conditions. Recent, dramatic improvements...Full Text Available

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Background"Nubiotics" are synthetic oligonucleotides and nucleotides with nuclease-resistant backbones, and are fully protonated for enhanced ability to be taken up by bacterial...Full Text Available

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Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

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... The CENTCOM AOR encompasses the world's most energy-rich region, with the Arabian Gulf region and Central Asia together ...

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Bacterial plasmids contain specific genes for resistances to toxic heavy metal ions including Ag+, AsO2-, AsO4(3-), Cd2+, Co2+, CrO4(2-), Cu2+, Hg2+, Ni2+, Pb2+, Sb3+, and Zn2+. Recent progress with...Full Text Available

UK PubMed Central (United Kingdom)

β-Lactamases are the main cause of bacterial resistance to penicillins and cephalosporins. Class A β-lactamases, the largest group of β-lactamases, have been found in many bacterial...Full Text Available

International Nuclear Information System (INIS)

A nuclear fuel waste disposal vault would not likely be a sterile environment. Bacterial activity would be expected in those areas of the vault conducive to bacterial life, i.e., where effects of heat, moisture content, radiation and compaction would not prevent or severely restrict bacterial life and where suitable and sufficient nutrients would be present. An inventory of bacterial nutrients that would be emplaced 'intentionally' with vault materials (fuel waste, waste containers, buffer and backfill materials) has been made previously. This report assesses bacterial nutrients that would be added 'inadvertently' to a vault in the form of residues of materials used to excavate and operate a vault. Measurements of blasting material residues in the various water supplies, excavated broken rock (muck) and in cores drilled in old and new tunnel walls were made at AECL's Underground ...

Science.gov (United States)

Bacterial attachment-effacement (att-eff) is emerging as an important virulence characteristic common to both enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). The contribution of the plasmid-encoded EPEC adherence factor to the production of mucosal lesions and diarrhea was investigated in gnotobiotic piglets. Bacterial att-aff in the intestinal mucosa of piglets infected with plasmid-cured EPEC strain E2348/69 (O127) was indistinguishable from that in piglets infected with the parent strain, but the distribution of lesions was different; it occurred in the small intestines of 6 of 7 piglets infected with the parent strain compared with only 2 of 11 (P = 0.006) infected with the plasmid-cured strain. Plasmid-encoded factors in EPEC and EHEC strains did not appear to contribute to bacterial competition with normal gut microflora. Of 13 strains belonging to five EPEC serogroups, O55, O142, O26, ...

Science.gov (United States)

Norepinephrine stimulates the growth of a range of bacterial species in nutritionally poor SAPI minimal salts medium containing 30% serum. Addition of size-fractionated serum components to SAPI medium indicated that transferrin was required for norepinephrine stimulation of growth of Escherichia coli. Since bacteriostasis by serum is primarily due to the iron-withholding capacity of transferrin, we considered the possibility that norepinephrine can overcome this effect by supplying transferrin-bound iron for growth. Incubation with concentrations of norepinephrine that stimulated bacterial growth in serum-SAPI medium resulted in loss of bound iron from iron-saturated transferrin, as indicated by the appearance of monoferric and apo- isoforms upon electrophoresis in denaturing gels. Norepinephrine also caused the loss of iron from lactoferrin. The pharmacologically inactive metabolite norepinephrine 3-O-sulfate, by contrast, did not result in ...

Energy Technology Data Exchange (ETDEWEB)

The growth power-time curves of a strain of petroleum bacteria, B-2, in various kinds of cultures containing different kinds of carbon sources, glucose, n-tetradecane, n-hexadecane and n-octadecane, and different kinds of microemulsions have been determined by using a 2277 Thermal Activity Monitor. The curves showed a single peak for cultures containing a single carbon source, glucose, and two peaks for cultures containing two kinds of carbon sources, glucose and one of the n-alkanes. The first peak indicated that bacteria grew by consuming glucose and the second peak indicated that bacteria grew by consuming n-alkane. The curves were complex when the bacterium grows in a microemulsion culture. According to a kinetic equation of bacterial growth under limited conditions, the rate constants of bacterial growth were obtained. The results showed that the microemulsion culture was more appropriate to bacteria to grow on n-alkanes.

Science.gov (United States)

Preterm birth is a common cause of neonatal morbidity and mortality. Many asymptomatic genital infections have been associated with preterm birth, but attempts to determine a causal relationship between specific infections and preterm birth have been disappointing. Treatment trials of specific infections have generally failed to show a positive effect, and in some trials have shown a deleterious effect. Although there is a strong association between the presence of bacterial vaginosis and Trichomonas vaginalis in pregnancy and preterm birth, randomized treatment trials have failed to show a benefit of treatment of these organisms. Treatment of asymptomatic bacterial vaginosis or T. vaginalis to prevent preterm birth is not warranted. PMID:12112946

British Library Electronic Table of Contents (United Kingdom)

Coral populations have precipitously declined on Caribbean reefs while algal abundance has increased, leading to enhanced competitive damage to corals, which likely is mediated by the potent allelochemicals produced by both macroalgae and benthic cyanobacteria. Allelochemicals may affect the composition and abundance of coral-associated microorganisms that control host responses and adaptations to environmental change, including susceptibility to bacterial diseases. Here, we demonstrate that extracts of six Caribbean macroalgae and two benthic cyanobacteria have both inhibitory and stimulatory effects on bacterial taxa cultured from the surfaces of Caribbean corals, macroalgae, and corals exposed to macroalgal extracts. The growth of 54 bacterial isolates was monitored in the presence of l...

UK PubMed Central (United Kingdom)

Although the roots of Ras sprouted from the rich history of retrovirus research, it was the discovery of mutationally activated RAS genes in human cancer in 1982 that stimulated an...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The ground state masses of thirty-nine neutron-rich nuclei from [sup 51]Ca to [sup 72]Ni have been measured using the Time-of-Flight Isochronous (TOFI) spectrometer. Eight of these masses have been measured for the first time and thirty-one are remeasurements of neutron-rich nuclei previously reported. Good agreement between these results and a previous TOFI experiment was observed except for the most neutron-rich isotopes of vanadium through iron with the present results being more bound and in better agreement with theory. The low binding energy of [sup 68]Ni, as indicated by an unreasonably low two-neutron separation energy, suggests the presence of a high-lying, long-lived isomeric state in this nucleus. (orig.)

UK PubMed Central (United Kingdom)

Fava beans (Vicia fava) are cultivated rather widely in most countries of the Eastern Mediterranean area and provide a cheap but protein-rich food that can be eaten alone, in various...Full Text Available

CERN Document Server

We report on aging measurements of a photon detector candidate for the HERA-B RICH. The prototype, a proportional wire chamber operated with TMAE, was mounted on an argon filled test-beam RICH and was aged by UV light at rates we expect in HERA-B. We monitored gain and current continuously, and checked the chamber with Cherenkov photons at regular intervals. The number of detected photons/track (projected to 2.75 m C sub 4 F sub 1 sub 0 in HERA-B) dropped from 26 to 11 after two days of aging - well below the 20 required for pi/K separation. Based on these results, HERA-B decided to use multi-anode PMTs as photon detectors in the RICH.

UK PubMed Central (United Kingdom)

Peri-prosthetic infections are notoriously difficult to treat as the biomaterial implant is ideal for bacterial adhesion and biofilm formation, resulting in decreased antibiotic sensitivity....Full Text Available

UK PubMed Central (United Kingdom)

Many bacterial pathogens produce extracellular proteases that degrade the extracellular matrix of the host and therefore are involved in disease pathogenesis. Dichelobacter nodosus...Full Text Available

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A previous report of high levels of members of the domain Archaea in Antarctic coastal waters prompted us to investigate the ecology of Antarctic planktonic prokaryotes. rRNA hybridization...Full Text Available

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The pathogenesis of Pseudomonas aeruginosa disseminated infections depends on bacterial interaction with blood vessels. We have hypothesized that in order to traverse the endothelial barrier, bacteria...Full Text Available

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Adaptive radiation is the rapid origination of multiple species from a single ancestor as the result of concurrent adaptation to disparate environments. This fundamental evolutionary process is considered...Full Text Available

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Type 1 pilus directs bladder epithelial binding and invasion by uropathogenic Escherichia coli (UPEC) in the initial stage of cystitis, but the bacterial determinants of postinvasion...Full Text Available

UK PubMed Central (United Kingdom)

Macrophage activation by the proinflammatory cytokine interferon-γ (IFN-γ) is a critical component of the host innate response to bacterial pathogenesis. However, the precise nature...Full Text Available

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Bacterial processes in soil, including biodegradation, require contact between bacteria and substrates. Knowledge of the three-dimensional spatial distribution of bacteria at the microscale is necessary...Full Text Available

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... were erroneous. The oriental rat flea, Xenopsylla cheopis Rothschild, is considered to be the primary vector of ... laboratory and production of Weigl's exanthematous typhus vaccine. In Maintenance of hum...

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The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically...Full Text Available

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To define the import pathway for apoiso-1-cytochrome c in vivo, the coding region for bacterial chloramphenicol acetyltransferase (CAT) or yeast copper metallothionein (CuMT) was fused to the carboxy...Full Text Available

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BackgroundThe human bacterial pathogen Campylobacter jejuni contains two subspecies: C. jejuni subsp. jejuni (Cjj)...Full Text Available

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Clindamycin concentrations in gingival crevicular fluid and in blood were determined over a 7-h period and were related to the minimal inhibitory concentrations of this agent for 340 bacterial strains...Full Text Available

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Zinc is an essential trace metal ion for growth, but an excess of Zn is toxic and microorganisms express diverse resistance mechanisms. To understand global bacterial responses to excess Zn, we conducted...Full Text Available

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BackgroundBerberine is a plant alkaloid that is widely used as an anti-infective in traditional medicine. Escherichia coli exposed to berberine form filaments, suggesting...Full Text Available

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Clostridium perfringens type A food poisoning is the second most commonly identified bacterial food-borne illness. Sporulation contributes to this disease in two ways: (i) most food-poisoning...Full Text Available

UK PubMed Central (United Kingdom)

Bacterial nitric oxide synthases (bNOS) are present in many Gram-positive species and have been demonstrated to synthesize NO from arginine in vitro and in vivo. However, the physiological role...Full Text Available

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Rumen-cannulated cows (n = 4) were fed successively silage made from either conventional or genetically modified (GM) maize. Results revealed no effects of GM maize on the dynamics...Full Text Available

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Many pathogens colonize different anatomical sites, but the selective pressures contributing to survival in the diverse niches are poorly understood. Group A Streptococcus (GAS) is...Full Text Available

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Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization,...Full Text Available

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In order to identify novel high value antibacterial targets it is desirable to delineate whether the inactivation of the target enzyme will lead to bacterial death or stasis. This knowledge is particularly...Full Text Available

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Biodegradation/solubilization of coal is described. The degradation of dibenzothiophene by two bacterial strains is reported. The effects of adding salicylate to the treatments was also investigated. 2 figs., 5 tabs. (CBS)

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BackgroundOceans are iron-deficient and nutrient-poor environments. These conditions impart limitations on our understanding of and our ability to identify microorganisms from the...Full Text Available

UK PubMed Central (United Kingdom)

In Iran, microscopic examination of skin scrapings from 2202 individuals with clinically diagnosed cutaneous leishmaniasis (CL) lesions revealed the presence of amastigotes in 1123 cases (51.0%). Bacteriological...Full Text Available

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The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin,...Full Text Available

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BackgroundSepsis or bacteraemia, however rare, is a significant cause of high mortality and serious complications in children. In previous studies skin disease or skin infections...Full Text Available

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The distribution and activity of communities of sulfate-reducing bacteria (SRB) and methanogenic archaea in two contrasting Antarctic sediments were investigated. Methanogenesis dominated in freshwater...Full Text Available

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BackgroundThe successful interaction of bacterial pathogens with host tissues requires the sensing of specific chemical and physical cues. The human gut contains a huge number of...Full Text Available

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Saccharococcus sacchari is the primary colonizer of the developing “sterile” tissue between the leaf sheath and stem of sugar cane. The honeydew secreted by the mealybugs...Full Text Available

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OBJECTIVE: The efficacy of Toto ointment and soap on common skin disorders was tested. METHODOLOGY: A cohort of Nigerians with common skin conditions such as fungal and bacterial skin infections, scabies,...Full Text Available

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A DNA fragment located on the 3' side of the Coxiella burnetii htpAB operon was determined by Southern blotting to exist in approximately 19 copies in the Nine Mile I genome. The DNA sequences of this...Full Text Available

International Nuclear Information System (INIS)

Surface-sensitive UV photoelectron yield spectroscopy was employed to study electron acceptor levels at surfaces of chalcopyrite structure Cu-In-Se thin films. Surface Fermi level pinning was observed for Cu-rich films. Shallow acceptor levels ascribable to defects Cu_I_n and V_C_u were observed for near-stoichiometric and In-rich films respectively. (orig.).

UK PubMed Central (United Kingdom)

HMGA2 is a DNA minor-groove binding protein. We previously demonstrated that HMGA2 binds to AT-rich DNA with very high binding affinity where the binding of HMGA2 to poly(dA-dT)₂ is enthalpy-driven...Full Text Available

International Nuclear Information System (INIS)

A unified calculation of neutron-rich isotopes in lithium is performed using the hyperspherical basis in which the underlying symmetry of each isotope exhibits a simple structure. The variation of the binding energy as a function of mass number is qualitatively reproduced, and the asymptotic of radial distribution of each isotope decreases exponentially. The form factors of the lithium isotopes are calculated and display diffraction minima. 27 refs., 3 figs., 3 tabs.

International Nuclear Information System (INIS)

Expression of Bacterial luciferase enzyme (lux) in mammalian cells would be a powerful bioreporter protein system for in vivo imaging because eukaryotic luciferases need expensive substrates. However, only a few efforts have been made to express bacterial luciferase enzyme in mammalian cells. As the result of this, we attempted to construct bicistronic vector including two bacterial luciferase genes (LuxA and LuxB) for assessing the potential to be visualized in vitro or in vivo by optical imaging system after transfection to mammalian cells. We designed and synthesized luxA and luxB genes from Photorhabdus Luminescens. To co-express both luxA and luxB genes from a single promoter, we cloned as a bicistronic transcript fused with an internal ribosomal entry site (IRES). This bicistronic transcript was transfected by Superfect to HEK 293T cell line. We also transfected lux A and lux B vector to HEK 293T cells separately. To ...

International Nuclear Information System (INIS)

A new two-body interaction recently derived for nuclei in the 0f1p shell by fitting two-body matrix elements to 494 energy levels in A=41-66 nuclei, is used to investigate the neutron-rich nuclei in the vicinity of the doubly closed nuclide "4"8Ca. This study is of fundamental interest in providing a test for the new effective interaction away from the stability line. Masses and binding energies are calculated for a variety of neutron-rich nuclei and compared with experimental data, where available. In addition level schemes for "5"0"-"5"2Ca, "5"1"-"5"2Sc and "5"1"-"5"2Ti have been calculated and are compared with available experimental data. In general a good correspondence between theory and experiment is found, but some systematic discrepancies are apparent. ((orig.)).

Energy Technology Data Exchange (ETDEWEB)

The characteristic rich shifting of the maximum adiabatic flame temperature from the stoichiometric value for mixtures of hydrocarbon and air is demonstrated to be caused by product dissociation and hence reduced amount of heat release. Since the extent of dissociation is greater on the lean side as a result of the stoichiometry of dissociated products, the peaking occurs on the rich side. The specific heat per unit mass of the mixture is shown to increase monotonically with increasing fuel concentration, and as such tends to shift the peak toward the lean side. It is further shown that this is the cause for the lean shifting of the adiabatic flame temperature of oxidizer-enriched mixtures of N{sub m}H{sub n} and F{sub 2} and of NH{sub 3} and O{sub 2}, with various amounts of inert dilution, even though their maximum heat release still peaks on the rich side. (author)

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This invention is comprised of an improved combustion system for an internal combustion engine is disclosed wherein a rich air/fuel mixture is furnished at high pressure to one or more jet plume generator cavities adjacent to a cylinder and then injected through one or more orifices from the cavities into the head space of the cylinder to form one or more turbulent jet plumes in the head space of the cylinder prior to ignition of the rich air/fuel mixture in the cavity of the jet plume generator. The portion of the rich air/fuel mixture remaining in the cavity of the generator is then ignited to provide a secondary jet, comprising incomplete combustion products which are injected into the cylinder to initiate combustion in the already formed turbulent jet plume. Formation of the turbulent jet plume in the head space of the cylinder prior to ignition has been found to yield a higher maximum combustion pressure in the ...

International Nuclear Information System (INIS)

Cultures of 3T3-L1 cells were incubated with either 10 ng/ml cholera toxin or 10 ng/ml pertussis toxin from 4 days prior to the initiation of differentiation and throughout the subsequent incubation. Toxin concentrations were sufficient to completely prevent the labelling of alpha-subunits with ["3"2P]NAD"+ and pertussis toxin and to prevent by more than 90% the labelling with ["3"2P]NAD"+ and cholera toxin in membranes prepared from these cells. Neither toxin prevented the differentiation to the adipocyte phenotype. Neither toxin prevented the increases in the relative amounts of G-proteins which occur upon differentiation. Both toxins dramatically decreased the amount of beta-subunits. As measured by quantitative immunoblotting with antisera specific for both the 35 kDa and 36 kDa beta-subunits, levels of beta-subunit were decreased by more than 50% of steady-state level of control cells. Thus, bacterial toxins which modifies G-protein alpha-subunits are capable ...

Science.gov (United States)

In 18 piglets, weighing 10-15 kg, third-degree burns or full-thickness skin excisions of 4 X 4 cm were inflicted. The effect of five dressing materials on adhesiveness to the wounds, appearance, conformability, wound contraction, bacterial count, and morphology of the wound was studied at the end of the seventh and fourteenth days without dressing changes. In 11 piglets with a burn wound, the most adherent dressing was collagen sponge(CS), followed by polyurethane sponge (PU), pigskin xenograph (PS), and xeroform. CS more effectively debrided the wound from coagulated necrotic tissue than the other dressings. Wound contraction was maximal with CS dressing (52%), followed by PU (44%), xeroform (32%), and PS (27%). In another seven piglets with full-thickness excised wounds, a velour dressing made of polytetrafluoroethylene (PTFE) or PU adhered significantly more than CS or PS. Wound contraction was greater with CS (37%) or PTFE (35%) than with PS (23%) or PU (18%). ...

Science.gov (United States)

A dialyzable factor(s) in human serum is known to stimulate gonococcal oxygen consumption. Its effect on other human pathogens was investigated. A 10% serum solution increased peak O2 consumption for Escherichia coli and Staphylococcus aureus to 157% (P less than 0.05) and 199% (P less than 0.02), respectively, of their O2 consumption when suspended in Hanks balanced salt solution, compared with a 356% increase for Neisseria gonorrhoeae with serum. Dialyzed serum lacked stimulatory capacity. Bacteria, serum, and neutrophils are often incubated to evaluate neutrophil bactericidal activity. Samples of 10(8) N. gonorrhoeae, S. aureus, and E. coli turned resazurin colorless (anaerobic conditions, Eh less than -42 mV) after 7.4, 13.3, and 15.1 min, respectively. Because neutrophil formation of reactive oxygen intermediates requires ambient O2, the effect of live bacteria and serum on this process was explored. After 5 min of incubation of 10(8) N. gonorrhoeae or S. aureus in 10% normal or ...

UK PubMed Central (United Kingdom)

The mammary gland is a complex tissue comprised of a branching network of ducts embedded within an adipocyte-rich stroma. The ductal epithelium is a bi-layer of luminal and myoepithelial cells, the...Full Text Available

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The ratio of particle velocity to translation speed exceeds 1 for 98% of .... The westward movement of the meandering jet and eddy system transports nutrient- rich ...... J. Hydrometeor., 4, 1044-1066. Gedney, N., P. M. Cox, H. Douville, ...

UK PubMed Central (United Kingdom)

1. The structural requirements of an allosteric barbiturate binding site on acetylcholine receptor-rich membranes isolated from Torpedo electroplaques have been characterized by the ability of fourteen...Full Text Available

UK PubMed Central (United Kingdom)

The genus of filamentous cyanobacteria, Lyngbya, has been found to be a rich source of bioactive metabolites. However, identification of such compounds from Lyngbya...Full Text Available

Science.gov (United States)

The 1985 evaluation of A = 1-1 (85B1114) has been revised. Experimental information is presented from the neutron-rich {sup 101}Sr to the neutron-deficient {sup 101}In.

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It is shown that a natural consequence of the binary pulsar's evolution is a neutron star collision. Such a collision is expected to eject neutron-rich matter of an r-process character. Taking reasonable estimates for the number of such events over the history of the galaxy, it may be that they account for all of the r-process nuclei.

Medline Plus

... also include a healthy diet that included abundant antioxidants, such as vitamin C, vitamin E, and lycopene. ... exfoliants, the higher-level moisturizers as well as antioxidants as my night product. A very rich moisturizer ...

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This book contains papers relating to the 1990 Future Transportation Technology Conference and Exposition. Included are the following papers; Examination of rich mixtures operation of a dual fuel engine, A hybrid natural gas vehicles, Performance analysis of a spark ignited engine with ETBE as a blending agent.

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This study tried to reduce NO{sub x} and particulate emissions simultaneously in a high speed direct-injection diesel engine. Fuel-rich combustion was used to reduce NO{sub x} emission at initial combustion stage and high turbulence combustion was used to reduce particulate emission at diffusion combustion stage. Experiments were carried out to investigate the effects of high squish combustion chambers with small throat on combustion process and exhaust emissions. Results showed that in the high squish combustion chamber, both NO{sub x} and particulate emissions reduced with retarded injection timing. Endoscopic high speed photography was employed to analyze experimental results. In the original combustion chamber at the early combustion stage, the high luminosity flame tended to spread out of the cavity. Fuel-rich mixture burned slowly over the piston crown. In the high squish combustion chamber, flame rotated violently around the bowl. ...

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Potential uses of foliage and bark wastes of E. ('Mysore') hybrid in southern India for the production of cineole and oxalic acid respectively are discussed. Only certain strains are cineole-rich and these are being successfully propagated vegetatively at the authors' laboratory.

UK PubMed Central (United Kingdom)

The authors' laboratory, using histochemicalmethods, previously identified two types of cholesterol-containing lipid particles in the extracellular spaces of human atherosclerotic lesions, one particle...Full Text Available

UK PubMed Central (United Kingdom)

Numerous epidemiological studies have consistently demonstrated that individuals who eat more fruits and vegetables (which are rich in carotenoids) and who have higher serum β-carotene levels...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Objective To compare matrix metalloproteinase (MMP)-8 and MMP-9 levels in the vaginal secretions of pregnant women with or without asymptomatic bacterial vaginosis (BV). Methods In this study, vaginal levels and molecular forms of MMP-8 and MMP-9 were studied in 36 pregnant women between 28 and 34 weeks of gestation with asymptomatic BV and 41 pregnant women, matched for gestational age, without BV. Results Vaginal MMP-8 concentrations were significantly higher (P = .023) in BV-positive women. There was no significant difference in MMP-9 levels between healthy pregnant controls and BV-positive pregnant women. The presence of MMP-8 was confirmed by a 38-kd band on Western blots. Conclusions Our findings show that BV is associated with increased levels of MMP-8 in vaginal fluid. Increased pr...

British Library Electronic Table of Contents (United Kingdom)

Eugenol, the principal chemical component of clove oil from Eugenia aromatica has been long known for its analgesic, local anesthetic, anti-inflammatory, and antibacterial effects. The interaction of the eugenol with ten different hydrophobic and hydrophilic antibiotics was studied against five different Gram negative bacteria. The MIC of the combination was found to decrease by a factor of 5-1000 with respect to their individual MIC. This synergy is because of the membrane damaging nature of eugenol, where 1mM of its concentration is able to damage nearly 50% of the bacterial membrane. Eugenol was also able to enhance the activities of lysozyme, Triton X-100 and SDS in damaging the bacterial cell membrane. The hydrophilic antibiotics such as vancomycin and b-lactam antibiotics which have ...

British Library Electronic Table of Contents (United Kingdom)

Abstract Plant pathogenic bacteria of the genus Xanthomonas cause a variety of diseases in economically important monocotyledonous and dicotyledonous crop plants worldwide. Successful infection and bacterial multiplication in the host tissue often depend on the virulence factors secreted including adhesins, polysaccharides, LPS and degradative enzymes. One of the key pathogenicity factors is the type III secretion system, which injects effector proteins into the host cell cytosol to manipulate plant cellular processes such as basal defense to the benefit of the pathogen. The coordinated expression of bacterial virulence factors is orchestrated by quorum-sensing pathways, multiple two-component systems and transcriptional regulators such as Clp, Zur, FhrR, HrpX and HpaR. Furthermore, virule...

Energy Technology Data Exchange (ETDEWEB)

This paper reports the results of leaching experiments conducted with and without Thiobacillus ferroxidans at the same conditions in solution. The extent of leaching of ZnS with Bacteria is significantly higher than that without bacteria at high concentrations of ferrous ions. A porous layer of elemental sulfur is present on the surfaces of the chemically leached particles, which no sulfur is present on the surfaces of the bacterially leached particles. The analysis of the data using the shrinking-core model shows that the chemical leaching of ZnS is limited by the diffusion of ferrous ions through the sulfur product layer at high concentrations of ferrous ions. The analysis of the data shows that diffusion through the product layer does not limit the rate of dissolution when bacteria are present. This suggests that the action of T.ferroxidans in oxidizing the sulfur formed on the particle surface is to remove the barrier to diffusion by ferrous ions.

British Library Electronic Table of Contents (United Kingdom)

Abstract The efficacy of iodine and glutaraldehyde as fish egg surface disinfectants were assessed in red porgy (Pagrus pagrus) and white sea bream (Diplodus sargus sargus) eggs, two species of interest for Mediterranean aquaculture. Iodine was effective in reducing the bacterial load of the 1-day-old eggs when applied at 50 mg L-1 for 5 min. The same concentration did not cause any significant change in hatching success or survival of the larvae for the first 5 days. Glutaraldehyde failed to reduce the bacterial load of the fish eggs at concentrations that were safe for the eggs (100 mg L-1 for 5 min), as it had a significant effect in preventing hatching of the developed embryo. Disinfecting 0-day-old eggs with iodine resulted in a significant reduction of hatching percentage, while larv...

British Library Electronic Table of Contents (United Kingdom)

Bacterial cellulose produced by the gram-negative bacterium Gluconacetobacter xylinum was found to be an excellent native starting material for preparing shaped ultra-lightweight cellulose aerogels. The procedure comprises thorough washing and sterilization of the aquogel, quantitative solvent exchange and subsequent drying with supercritical carbon dioxide at 40 degreeC and 100 bar. The average density of the obtained dry cellulose aerogels is only about 8 mg cm-3 which is comparable to the most lightweight silica aerogels and distinctly lower than all values for cellulosic aerogels obtained from plant cellulose so far. SEM, ESEM and nitrogen adsorption experiments at 77 K reveal an open-porous network structure that consists of a comparatively high percentage of large mesopores and small...

Energy Technology Data Exchange (ETDEWEB)

The isomeric and {beta}-decay properties of neutron-rich {sup 53-57}Sc and {sup 53,54}Ca nuclei near neutron number N = 32 are reported, and the low-energy level schemes of {sup 53,54,56}Sc and {sup 53-57}Ti are presented. The low-energy level structures of the {sub 21}Sc isotopes are discussed in terms of the coupling of the valence 1f{sub 7/2} proton to states in the corresponding {sub 20}Ca cores. Implications with respect to the robustness of the N = 32 subshell closure are discussed, as well as the repercussions for a possible N = 34 subshell closure.

International Nuclear Information System (INIS)

The effect of the rare earth element, yttrium, on the mechanical properties of 9Cr-2WVTa low active martensite steel for fusion reactor was studied and the metallurgical behavior of yttrium in the steel was primarily analyzed. The results show that it is easy for yttrium to aggregate and form the blocky yttrium rich inclusions in the steel, which can dissever the continuity of the matrix and produce micro-cracks for fracture. The yttrium rich inclusions were distributed along the rolling direction, which made the fracture surface delaminated in the tensile and impact samples, and reduced the mechanical properties of the steel. (authors)

International Nuclear Information System (INIS)

Beta-decay studies of the new neutron-rich isotopes sup(58,59)Cr, "6"3Mn, sup(66,67)Co and "6"9Ni, yielding distinctly shorter half-lives than the corresponding theoretical predictions, are presented. The influence of the short half-lives on the r-process calculations of nuclear abundances is discussed. It is concluded that a significantly higher neutron density than the one obtained from explosive helium burning in supernovae is needed to reproduce the observed abundances around A = 80. (orig.).

Science.gov (United States)

The authors describe an effect of suppression of invasion of the guinea pig eye conjunctiva and the HEp-2 epithelial cells by virulent Sh. flexneri bacilli, with a simultaneous administration of the same dose of avirulent shigella mutants, genetically connected with them. The data of morphological study and experiments with 3H-glucose labeled shigellae carried out on the cell species model indicated that the bacterial competition for the specific sites for absorption on the epithelial cells underlay the observed phenomenon. PMID:331774

UK PubMed Central (United Kingdom)

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Our work is towards mechanistically understanding interactions of unsaturated bacterial biofilms and their extracellular polymeric substances (EPS) with actinide metals and metal surrogates under vadose zone conditions. Because metal contaminants in the vadose zone co-occur with organic pollutants, some of our work has included experiments with organic pollutants.

Science.gov (United States)

We introduce a discrete multiplicative process as a generic model of competition. Players with different abilities successively join the game and compete for finite resources. Emergence of dominant players and evolutionary development occur as a phase transition. The competitive dynamics underlying this transition is understood from a formal analogy to statistical mechanics. The theory is applicable to bacterial competition, predicting novel population dynamics near criticality.

UK PubMed Central (United Kingdom)

The positive control function of the bacterial enhancer-binding protein NtrC resides in its central domain, which is highly conserved among activators of ς⁵⁴ holoenzyme. Previous...Full Text Available

International Nuclear Information System (INIS)

Intrahepatic cholangiocarcinoma is the second most common primary malignant hepatic neoplasm. We describe two cases of intrahepatic cholangiocarcinoma which initially presented as liver abscess both clinically and radiologically. Mucin-hypersecretion from the tumor cells and extensive necrosis or secondary bacterial infection was responsible for the radiologic appearance of a liver abscess.=20

UK PubMed Central (United Kingdom)

PurposeCoexistence of different classes of β-lactamases in a single bacterial isolate may pose diagnostic and therapeutic challenges. We investigated a spread of Klebsiella...Full Text Available

Science.gov (United States)

Campylobacter spp. are present in the intestinal tract and internal tissues of broiler breeder and broiler chickens. Campylobacter spp. are known to cause acute bacterial gastroenteritis in humans and raw poultry products have been implicated as a significant source of these infections. The object...

UK PubMed Central (United Kingdom)

BackgroundThermostable bacterial lipases occupy a place of prominence among biocatalysts owing to their novel, multifold applications and resistance to high temperature and other...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Significant ion irradiation during film growth is required for the formation of cubic boron nitride (cBN) films. Meanwhile, a huge level of intrinsic stress possibly induced by the ion bombardment has been frequently reported to result in cracking and/or lack of adhesion of deposited cBN films. The present work has been performed to investigate the interfacial and/or the buffer layer structures with better matching to the cBN film by relaxation of the film stress using ion-beam-assisted deposition (IBAD). Boron nitride films have been synthesized on Si(100) wafer and tungsten carbide (WC) substrates by depositing boron vapor under simultaneous bombardment with nitrogen ions and nitrogen-argon mixture ions in the energy range of 0.5-10 keV. Cubic BN films with enhanced tribological properties have been explored by inserting a BN layer with various B/N compositions as a controlled buffer at the interface. Significant relaxation of the film stress has been observed for the buffer layer ...

Science.gov (United States)

The neutron-rich Mn isotopes from A=59 to 63 have been studied through multi-nucleon transfer reactions by bombarding a {sup 238}U target with a beam of {sup 70}Zn at an energy of E{sub lab}=460 MeV. Prompt {gamma} rays measured by the CLARA array have been identified unambiguously for each nucleus, using coincidence relationships with ions detected in the high-acceptance magnetic spectrometer PRISMA. The new data extends the knowledge of the low-lying level structure of Mn isotopes, which is discussed in terms of the systematics of the region. Results are compared with large-scale shell-model calculations using different effective interactions and valence spaces.

British Library Electronic Table of Contents (United Kingdom)

Monthly sampling of slightly alkaline arsenic-rich stream in the Mokrsko gold deposit revealed seasonal variations in dissolved Zn, Cu, As and Mo. Concentrations of trace metal cations (Zn, Cu) increased as much as 330 and 178%, respectively, from minimum mean values at autumn to maximum mean values at spring. In contrast, concentrations of trace element oxyanions (As, Mo) revealed opposite seasonal pattern with increase to 189% (As) and 123% (Mo) during summer?autumn, indicating that in-stream biogeochemical process(es) played the main role in controlling the seasonal variations of these trace elements. The trace elements were mainly scavenged by low crystalline Mn oxyhydroxide and Fe oxyhydroxide (ferrihydrite). Results are consistent with sorption and coprecipitation processes controlli...

Energy Technology Data Exchange (ETDEWEB)

We have measured production cross-sections of the new neutron-rich isotopes {sup 58}Ti, {sup 61}V, {sup 63}Cr, {sup 66}Mn, {sup 69}Fe, {sup 71}Co and neighbouring isotopes that have been identified as projectile fragments from reactions between a 500 MeV/u {sup 86}Kr beam and a beryllium target. The isotope identification was performed with the zero-degree magnetic spectrometer FRS at GSI, using in addition time-of-flight and energy-loss measurements. The experimental production cross-sections for the new nuclides and neighbouring isotopes are compared with an empirical parametrization. The resulting prospects for reaching even more neutronrich isotopes, such as the doubly-magic nuclide {sup 78}Ni, are discussed. (orig.).

International Nuclear Information System (INIS)

Two small craters (number 74, 119 microns, and number 31, 158 microns in diameter) with depth to diameter ratios of about 0.59 and 0.8, respectively, were found in Al from the Long Duration Exposure Facility (LDEF) experiment tray A11EOOF. Both craters have residues concentrated in the crater bottoms, along the walls, and on top of the overturned rims. Low voltage scanning electron electron microscopy, Auger electron spectroscopy, time of flight secondary ion mass spectroscopy and energy dispersive x-ray spectroscopy were used to obtain high resolution imagery and elemental analysis. Analyses indicate that the impactor for both craters was carbon-rich, as the residues contain mostly C. Silicon, S, and F in low concentrations are present on the Al surface away from the craters and may be, in part, contaminants.

Energy Technology Data Exchange (ETDEWEB)

The effect of Mo addition as an alloying element to stainless steel alloys is investigated by capacitance (Mott Schottky approach), and photoelectrochemistry measurements. Complementary studies were made using Auger electron spectroscopy and X-ray photoelectron spectroscopy. The Mott-Schottky approach and the photoelectrochemical studies showed that the presence of Mo as an alloying element affects the semiconductive properties of the oxide films. The analytical results have shown that the oxide films formed on stainless steels are composed by an external Fe rich region and an inner Cr rich region. No significant amount of Mo was found in the outer layers of the film. The presence of Mo leads to an increase of the chromium content in the inner layers of the film, although without increasing the film thickness. (orig.) 30 refs.

British Library Electronic Table of Contents (United Kingdom)

The transformation mechanism of hexagonal delta phase from the disordered bcc gamma phase has not been reported before in the Zr-rich U-Zr alloy system. With the help of X-ray diffraction, transmission electron microscopy (TEM) and high-resolution TEM analyses it was shown that the gamma to delta conversion takes place by the lattice collapse mechanism of omega transformation. It was also ascertained that a higher aging temperature or time promotes the growth of all four variants of the delta phase within a parent gamma grain. In addition, ab initio electronic structure calculations showed that the bcc to hexagonal transformation, involving partial ordering of the parent bcc phase followed by (111) plane collapse, is energetically favorable.

International Nuclear Information System (INIS)

The talk will focus on spin-dependent competition between oblate and prolate shape minima in the potential energy landscape of "1"8"0Hf (the most neutron-rich stable isotope), mediated via the alignment of valence nucleons. Results of a prompt spectroscopic study, using deep inelastic reactions with Gammasphere and CHICO, bombarding a thin "2"3"2Th target with a "1"8"0Hf beam #approx#25% above the Coulomb barrier, will be presented. Nucleon alignments in both prolate and oblate minima will be discussed, as well as the favoring of oblate collective rotation at high spins, observed through a mixing with gamma vibrations built on the prolate shape.

CERN Document Server

A prism spectrometer has been developed to operate in the VUV wavelength range from 120 to 200 nm. It can be used as a pre- disperser in conjunction with a Fabry-Perot based gas refractometer. This instrument has also been used to measure the refractive index of the liquid radiator C/sub 6/F/sub 14/ in various spectral lines. This radiator is used in the RICH detectors of the DELPHI experiment and has been proposed for ALICE, and LHCb experiments. The spectral resolution of the system is improved as the wavelength decreases and the data are consistent with a wavelength accuracy about 0.4 nm at 140 nm. The results for the dispersion curve of the above liquid are presented. (17 refs).

Science.gov (United States)

Products of a power plant flue gas desulfurization scrubber are discharged into a pond as sludge consisting of calcite (initial delta13C 3.2-3.8 per thousand), gypsum (initial delta34S 7.6-8.6 per thousand), and aqueous solution. Reducing conditions exist below a boundary that appears to move vertically as a function of changes in pond water level. Under reducing conditions, bacteria partially reduce aqueous sulfate to low-delta34S sulfide, consuming organic carbon and generating low-delta13C bicarbonate. Under oxidizing conditions, sulfide is converted to sulfate, leading to calcite dissolution, gypsum precipitation, and isotopic re-equilibration of remaining calcite with dissolved bicarbonate near the pond surface. The gypsum has delta34S near 6 per thousand, and calcite has delta13C as low as -1.7 per thousand; the changes from initial values correspond to predictions based on isotopic balance and reaction stoichiometry. The pond largely contains the products of ...

Energy Technology Data Exchange (ETDEWEB)

The origin of natural gas in the Japanese oil and gas fields are geochemically studied. Samples are taken from structural natural gas, surface gas seepage, water-dissolved natural gas and coal-field gas of the Japan Sea coast area. The origins of primary hydrocarbons are classified into bacteria gas and thermogenic gas, the latter being subclassified into gas in oil production zone with the per million deviation of the carbon isotope (/sup 13/C/sup 1/) of methane less than -35 and those gases with maturity higher than this. Surface gas seepage is subjected to migration and bacterial oxidation. Coal gas is similar to oil gas. The CO/sub 2/ concentration is 0 - 2% and /sup 13/CO/sub 2/ deviations from -30 - +30. The origin can be barely estimated from the isotopic composition because of the wide secondary change in the composition. The origin and migration of gas from the gas field of northern Niigata are not similar to those in the southern area, which consist of a ...

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The genus Conexibacter (Monciardini et al. 2003) represents the type genus of the family Conexibacteraceae (Stackebrandt 2005, emend. Zhi et al. 2009) with Conexibacter woesei as the type species of the genus. C. woesei is a representative of a deep evolutionary line of des-cent within the class Actinobacteria. Strain ID131577T was originally isolated from temperate forest soil in Gerenzano (Italy). Cells are small, short rods that are motile by peritrichous fla-gella. They may form aggregates after a longer period of growth and, then as a typical charac-teristic, an undulate structure is formed by self-aggregation of flagella with entangled bacteri-al cells. Here we describe the features of the organism, together with the complete sequence and annotation. The 6,359,369 bp long genome of C. woesei contains 5,950 protein-coding and 48 RNA genes and is part of the Genomic Encyclopedia of Bacteria and Archaea project.

International Nuclear Information System (INIS)

The effect of UV radiation in the wavelength region 230 nm to 302 nm on the ability of an irradiated mammalian cell to reactivate UV-irradiated mammalian virus was tested. An action spectrum for radiation enhanced reactivation (RER) is presented. The shape of the action spectrum points to a combined nucleic acid-protein target for UV radiation effects on this cellular parameter. An analysis of the results of others involving the biochemical and photobiological events involved in RER does not allow us to distinguish which macromolecule is the major contributor to this effect. Studies involving an analogous phenomenon in bacteris (Weigle reactivation) imply that RER and WR amy involve similar mechanisms. (author).

Energy Technology Data Exchange (ETDEWEB)

The microbial safety of foods cooked in microwave ovens was investigated. The mechanisms of microwave destruction of microorganisms were examined. Effects of time and temperature on microorganisms in different food systems were described. Studies showed that: microwave heating of food is more ''''food dependent'' than conventional heating; recommended microwave treatment time for some foods may not destroy high levels of bacteria; use of microwaves in combination with conventional heating methods results in more uniform heating of foods and destruction of bacteria; and microwaves exert different killing effects on individual bacterial species. (78 references, 2 tables)

International Nuclear Information System (INIS)

The analgesic, dipyrone (1,phenyl-2,3-dimethyl-5-pyrazolone-4-methylamino methane sulphonate sodium), at 20 mM concentration, inhibited the rejoining of single-strand scissions in DNA of Escherichia coli B/r cells induced by 20 krad gamma-radiation. The chemical altered the cell membrane structure as evidenced from the uptake of acriflavin, the efflux of potassium ions from the bacterial cells and the inhibition of alkaline phosphatase-a cell membrane associated enzyme. (author).

Energy Technology Data Exchange (ETDEWEB)

Sulfur K-edge X-ray absorption spectroscopy is a powerful in situ probe of sulfur biochemistry in intact cells and tissues. Under favorable circumstances the technique can provide quantitative information on the chemical identify of the sulfur species that are present in a sample. Prange et al. have recently reported an X-ray absorption spectroscopic study of bacterial sulfur storage globules. Unfortunately there are substantial problems with the experimental technique employed that, they contend, lead to completely erroneous conclusions. In the more recent of their two papers Prange et al. employed a curve-fitting method similar to that used by us (for more than 10 years). In essence, the method employs simply fitting a linear combination of the spectra of standard compounds to that of the unknown, in this case cultures of bacterial cells. This type of analysis can provide quantitative estimates of the individual sulfur types in the sample, ...

Energy Technology Data Exchange (ETDEWEB)

This thesis contains the candidate's original work on excitonic structure and energy transfer dynamics of two bacterial antenna complexes as studied using spectral hole-burning spectroscopy. The general introduction is divided into two chapters (1 and 2). Chapter 1 provides background material on photosynthesis and bacterial antenna complexes with emphasis on the two bacterial antenna systems related to the thesis research. Chapter 2 reviews the underlying principles and mechanism of persistent nonphotochemical hole-burning (NPHB) spectroscopy. Relevant energy transfer theories are also discussed. Chapters 3 and 4 are papers by the candidate that have been published. Chapter 3 describes the application of NPHB spectroscopy to the Fenna-Matthews-Olson (FMO) complex from the green sulfur bacterium Prosthecochloris aestuarii; emphasis is on determination of the low energy vibrational structure that is important for ...

Energy Technology Data Exchange (ETDEWEB)

Spice extracts under the form of essential oils were tested for their efficiency to increase the relative radiosensitivity of Listeria monocytogenes and Escherichia coli O157H7 in culture media. The two pathogens were treated by gamma-irradiation alone or in combination with oregano essential oil to evaluate their mechanism of action. The membrane murein composition, and the intracellular and extracellular concentration of ATP was determined. The bacterial strains were treated with two irradiation doses: 1.2 kGy to induce cell damage and 3.5 kGy to cause cell death for L. monocytogenes. A dose of 0.4 kGy to induce cell damages, 1.1 kGy to obtain viable but nonculturable (VBNC) state and 1.3 kGy to obtain a lethal dose was also applied on E. coli O157H7. Oregano essential oil was used at 0.020% and 0.025% (w/v), which is the minimum inhibitory concentration (MIC) for L. monocytogenes. For E. coli O157H7, a concentration of 0.006% and 0.025% (w/v) which is the ...

International Nuclear Information System (INIS)

We report on a time-resolved measurement of self-amplified spontaneous emission free-electron laser (FEL) pulses. We observed that the spikes in such FEL pulses have an intrinsic positive chirp and the energy chirp in the electron bunch mapped directly into the FEL output. The measurement also provides rich information on the statistics of the FEL pulses.

Energy Technology Data Exchange (ETDEWEB)

Research is presented on oil and gas technologies, advanced systems, soil remediation and remediation of acid mine drainage, applied science research in plastics and petroleum coprocessing, and fossil fuel and hydrocarbon conversion using a hydrogen rich plasmas.

UK PubMed Central (United Kingdom)

Plant roots release about 5% to 20% of all photosynthetically-fixed carbon, and as a result create a carbon-rich environment for numerous rhizosphere organisms, including plant pathogens and symbiotic...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The cosmic antiparticle ring imaging Cherenkov experiment (CAPRICE) flew on a stratospheric balloon 8-9 August 1994 over northern Canada and collected data for more than 21 hours with less than 5 g/cm{sup 2} of residual atmosphere. The instrument includes a solid radiator RICH detector and an electromagnetic calorimeter for particle identification in the magnetic spectrometer. Preliminary antiproton and positron identification capabilities are presented.

Energy Technology Data Exchange (ETDEWEB)

China is continuing to extract oil from kerogen shale despite having a crude production capacity of 100 Mt a year. Among the reasons for this policy are the country's rich oil shale resources, comparatively mature extraction techniques, and a comprehensive utilization of the shale that has proved to be economically profitable. China has oil shale deposits of 340 Gt, equivalent to 24.5 Gt of shale oil, to rank fourth in the world after the U.S., Brazil, and the Soviet Union.

Energy Technology Data Exchange (ETDEWEB)

The fragment separator at GSI, Darmstadt, has been used to produce and separate very proton rich nuclei in the {sup 100}Sn region. By fragmentation of a {sup 112}Sn beam at 1 A{center_dot}GeV we produced nuclei along the rp-process path between {sup 77}Y and {sup 98}In. By implanting these ions into a silicon detector stack we were able to determine their halflives. Preliminary data are presented.

UK PubMed Central (United Kingdom)

Rapidly developing changes in the short-circuiting current (Isc), conductance (G), and potential (PD) of turtle bladders in Na-rich or Na-free media are seen after the mucosal addition, at 10 nM, of...Full Text Available

UK PubMed Central (United Kingdom)

An unusual S1-nuclease sensitive microsatellite (STMS) has been found in the single copy, rat polymeric immunoglobulin receptor gene (PIGR) terminal exon. In Fisher rats, elements within or beyond the...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The ..beta.. decay half-lives of neutron-rich isotopes with Z=24-28 are calculated in the QRPA with a Gamow-Teller residual interaction. For odd-mass and odd-odd systems QRPA phonon correlations are introduced to quasiparticle transitions in first-order perturbation. The calculated half-lives agree very well with the experimental values. For later application of this model to nuclei far from stability, we have examined the dependence of the calculated half-lives on the model parameters.

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The Tevatron collider at Fermilab provides a very rich environment for the study B{sub s} mesons. In this paper they show a few selected topics from the CDF and D0 collaborations, giving special attention to the B{sub s} Mixing analyses. This note corresponds to the proceedings of the Hadron Collider Physics 2005 conference.

International Nuclear Information System (INIS)

Iridium concentration in extra-terrestrial bodies is an important quantity in relation to Ir-rich geological layers. Ir concentration of a Yamato carbonaceous chondrite (Y-793321) has been measured by a neutron activation method. The measurement yields a value of (0.57#+-#0.06) #mu#g per gramme for the chondrite. 4 refs.; 1 figure.

Science.gov (United States)

Elution profiles of total lipoproteins, apolipoprotein B (apoB) concentrations in lipoproteins, and plasma triglyceride (TG) levels were examined in early-, late-, and non-lactating cows. Additionally, arteriovenous (A-V) differences were also measured to elucidate the uptake of TG and apoB-containing lipoproteins in mammary gland. Non-lactating cows showed three major peaks corresponding to triglyceride-rich lipoprotein (TRL), low density lipoprotein (LDL), and high density lipoprotein (HDL) fraction, whereas both early- and late-lactating cows revealed two peaks corresponding to TRL and HDL. The peak area of TRL in early- and late-lactating cows were significantly (p < 0.05) smaller than that in non-lactating cows. The plasma TG levels and apoB-48 concentrations of TRL in early- and late-lactating cows were also significantly (p < 0.01) lower. Furthermore, early lactating cows showed significantly (p < 0.05) larger A-V differences in both plasma TG and ...

International Nuclear Information System (INIS)

We measure the transient enhanced diffusion of shallow molecular-beam-epitaxy grown marker layers of Sb and B due to deep MeV Si"+ ion implants at very high doses (#approx#10"1"6cm"-"2). We expect the near-surface region of these implants to be vacancy rich, and we observe transient enhanced diffusion of Sb (the classic vacancy diffuser). The large enhancements imply a significant vacancy supersaturation (#approx#700 at 740 degree C). Double implantation of the high-dose MeV Si followed by a shallow (40 keV) Si implant and annealing produces a greatly reduced number of #left brace#311#right brace# defects compared to a 40 keV implant into virgin Si, again consistent with a vacancy-rich region in the near-surface region of an MeV implant. However, the shallow B marker layers also show transient enhanced diffusion for the same MeV implant under similar annealing conditions, implying that an interstitial supersaturation is present at the same ...

International Nuclear Information System (INIS)

Seeing convolved two-dimensional de Vaucouleurs profiles have been fitted to digital images of the brightest elliptical galaxies in two nearby groups of galaxies and three clusters of galaxies with redshifts in the range 0.06 to 0.15. The digital images were created by scanning electronographic plates obtained by the use of several versions of the RGO electronographic camera. The linear relation between effective surface brightness #mu#/sub e/ and the logarithm of the effective radius r/sub e/, first discovered by Kormendy to be valid for nearby elliptical galaxies, is found to be equally valid for the largest and brightest galaxies in rich clusters of galaxies. The apparent universal nature of that relation indicates that the same type of processes might have been involved during the formation of all ellipticals from cD galaxies in clusters to fainter ellipticals in small groups. It is suggested that the (I/sub e/,r/sub e/) relation is not changed by the expected ...

CERN Document Server

This paper presents new images and spectroscopy of NGC 34 (Mrk 938) obtained with the du Pont 2.5-m and Baade 6.5-m telescopes at Las Campanas, plus photometry of an HST archival V image. This Mv = -21.6 galaxy has often been classified as a Seyfert 2, yet recently published infrared spectra suggest a dominant central starburst. We find that the galaxy features a single nucleus, a main spheroid containing a blue central disk, and tidal tails indicative of two former disk galaxies. These galaxies appear to have completed merging. The remnant shows three clear optical signs that the merger was gas-rich ("wet") and accompanied by a starburst: (1) It sports a rich system of young star clusters, of which 87 have absolute magnitudes -10.0 > Mv > -15.4. Five clusters with available spectra have ages in the range 0.1-1.0 Gyr, photometric masses between 2x10^6 and 2x10^7 Msun, and are gravitationally bound young globulars. (2) The blue central ...

International Nuclear Information System (INIS)

Superconducting Cu-rich composites containing the A-15 compounds V_3Si or V_3Ga were made by the ''Tsuei'' process (melting into ingots followed by cold working and heat treatment). Superconducting transition temperatures of the composites were measured. X-ray diffraction analyses were performed. Microstructures were studied using both the optical metallograph and the scanning electron microscope. For some composites containing V_3Ga, the critical current densities as functions of transverse magnetic field up to 60 kG, and as functions of temperature from 4.2 to 12"0K were measured. It was found that the Tsuei process does not work for the composites containing V_3Si, but works satisfactorily for V_3Ga; reasons are discussed. Relations between measured properties and various metallurgical factors such as alloy compositions, cross-section reduction ratios, and heat treatment are discussed. The mechanism for the observed superconductivity in the materials is also ...

Energy Technology Data Exchange (ETDEWEB)

This study investigated the engine performance and emissions of a supercharged dual-fuel engine fueled by hydrogen-rich coke oven gas and ignited by a pilot amount of diesel fuel. The engine was tested for use as a cogeneration engine, so power output while maintaining a reasonable thermal efficiency was important. Experiments were carried out at a constant pilot injection pressure and pilot quantity for different fuel-air equivalence ratios and at various injection timings without and with exhaust gas recirculation (EGR). The experimental strategy was to optimize the injection timing to maximize engine power at different fuel-air equivalence ratios without knocking and within the limit of the maximum cylinder pressure. The engine was tested first without EGR condition up to the maximum possible fuel-air equivalence ratio of 0.65. A maximum indicated mean effective pressure (IMEP) of 1425 kPa and a thermal efficiency of 39% were obtained. However, the nitrogen ...

Energy Technology Data Exchange (ETDEWEB)

In this review, the most important inorganic natural gels are presented: opal, aluminosilicate (allophanes) and hydrous iron oxides and silicates. It is demonstrated that natural gels are ordered at the atomic scale. In allophanes, Al is distributed between octahedral and tetrahedral sites. The amount of Al increases as Al/Si ratio decreases. Si-rich allophane have a local structure around Al and Si very different of that is known in kaolinite or halloysite. Transformation of Si-rich allophanes to crystallized minerals implies dissolution-recrystallization processes. On the contrary, in iron silicate with Fe/Si = 0.72, Si and Fe environments are close to those found in nontronite. The gel transformation to Fe-smectite may occur by long range ordering during ageing. In ferric silicate gels, the similarity of local structure around Fe in poorly ordered precursors and what is known in crystallized minerals suggests a solid transformation during ...

Energy Technology Data Exchange (ETDEWEB)

The masses of the proton-rich nuclides {sup 85,86,87}Mo and {sup 87}Tc have been measured with the Penning trap mass spectrometer SHIPTRAP at GSI. The measured mass excesses of these nuclei deviate from the values of the Atomic Mass Evaluation 2003 by up to 1.6 MeV, indicating a systematic shift of the mass surface in this region compared to previous measurements. A local mass extrapolation based on the new results has been made for the mass range A=80-95. Measured and extrapolated mass values and the derived separation energies have been compared to theoretical mass models. Taking into account the new mass excess values, rp-process network calculations have been performed. Preliminary results show changes in the final abundances for A=86-92 by up to a factor of 30. Results of measurements and calculations are presented, and the issue of a potential Zr-Nb cycle is addressed.

International Nuclear Information System (INIS)

We studied the magnetic dipole moments #mu# of even-odd C isotopes, ranging from proton-rich to neutron-rich nuclei, with antisymmetrized molecular dynamics (AMD). The results are in good agreement with the experimental data. In the "9C ground state the total intrinsic spin of the protons is found to be nonzero (S_p#not =#0), which is unusual in even-odd nuclei. The interesting point is that the spin-orbit force breaks slightly the coupling off of intrinsic spins of the even nucleon group in isospin T=3/2 nuclei. This result is consistent with the newly measured #mu# data that, when combined with "9Li data, indicate an unusual left-angle #sigma# right-angle value larger than unity. A #mu# moment -1.05#mu#_N of "1"7C is theoretically predicted. We also show a good reproduction of E2 transition data. copyright 1996 The American Physical Society.

British Library Electronic Table of Contents (United Kingdom)

We describe a method for the analysis of multi-site phosphorylation in serine/threonine (Ser/Thr)-rich protein sequences. Site-specific mutagenesis was used to introduce tryptic cleavage sites in the serine glutamine/threonine glutamine cluster domain (SCD) of the human checkpoint protein kinase (Chk2). The mutant proteins were shown to autophosphorylate on residues that are inducibly phosphorylated when mammalian cells are exposed to ionizing radiation (serine 33/35, serine 516, threonine 68 and threonine 432). Five Ser/Thr clusters within the SCD were flanked by arginine or lysine residues to produce tryptic peptides for nanospray liquid chromatography (nanoLC)/linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Phosphorylation sites were assigned usin...

CERN Document Server

HD 100453 has an IR spectral energy distribution (SED) which can be fit with a power-law plus a blackbody. Previous analysis of the SED suggests that the system is a young Herbig Ae star with a gas-rich, flared disk. We reexamine the evolutionary state of the HD 100453 system by refining its age (based on a candidate low-mass companion) and by examining limits on the disk extent, mass accretion rate, and gas content of the disk environment. We confirm that HD 100453B is a common proper motion companion to HD 100453A, with a spectral type of M4.0V - M4.5V, and derive an age of 10 +/- 2 Myr. We find no evidence of mass accretion onto the star. Chandra ACIS-S imagery shows that the Herbig Ae star has L_X/L_Bol and an X-ray spectrum similar to non-accreting Beta Pic Moving Group early F stars. Moreover, the disk lacks the conspicuous Fe II emission and excess FUV continuum seen in spectra of actively accreting Herbig Ae stars, and from the FUV continuum, we find the ...

Energy Technology Data Exchange (ETDEWEB)

The masses of proton rich nuclides in the vicinity of N=Z=43 were measured with the Penning trap mass spectrometer SHIPTRAP at GSI. These nuclei were produced in the fusion-evaporation reaction {sup 36}Ar+{sup 54}Fe at energies of 5.0 and 5.9 MeV/u and separated at the velocity filter SHIP. The data are of astrophysical interest since these nuclei are believed to be a part of the rp and {nu}p process paths. The masses of {sup 85}Mo and {sup 87}Tc were measured for the first time. The masses of another two nuclides, {sup 86,87}Mo, were determined for the first time in a direct mass measurement. For these nuclides the mass excess deviates from values of the 2003 Atomic Mass Evaluation by up to 1.5 MeV, indicating a systematic shift of the mass surface in this region of the nuclear chart. Additionally, the masses of {sup 86}Zr and {sup 85}Nb were measured and found to be in agreement with the values obtained at JYFLTRAP. The experiment as well as preliminary data on ...

Energy Technology Data Exchange (ETDEWEB)

Sulfur pollution can lead to serious problems in freshwater wetlands, including phosphorus eutrophication and sulfide toxicity. We tested the effects of anaerobic iron-rich groundwater discharge in fens, simulated by iron injection, on two characteristic species (Juncus effusus and Caltha palustris) in a sulfidic environment. Biomass production of C. palustris roots showed an optimum response to the combined addition of iron and sulfide, with highest values at intermediate concentrations of both substances. Iron deficiency apparently occurred at low iron concentrations, while at high iron concentrations, growth was decreased. For J. effusus, in contrast, no toxic effects were found of both iron and sulfide. This could be explained by larger radial oxygen loss (ROL) of J. effusus and could not be explained by differences in phosphorous concentrations. The results of our experiments confirm that iron-rich groundwater discharge has the potential ...

International Nuclear Information System (INIS)

The effect of a carbohydrate, a 20% protein, or a carbohydrate +0.3% tryptophan TRP breakfast on plasma large neutral amino acid ratio was studied in 6 healthy men. The carbohydrate-rich meal produced shifts in plasma amino acid concentrations such that plasma TRP/LNAA ratios increased from 0.13 to 0.15 (p<0.04) and the protein meal decreased the ratio from 0.14 to 0.11 (p<0.04) after 1 hour. Addition of 0.3% TRP to the carbohydrate-rich meal increased plasma TRP/LNAA ratios more than 2-fold. The TRP containing meal was thus the only one likely to influence brain 5-HT synthesis, although the difference between the plasma TRP/LNAA ratios after carbohydrate and protein breakfasts suggests that the brain may distinguish, by synthesizing more or less 5-HT, the composition of breakfast meals. (Author).

Energy Technology Data Exchange (ETDEWEB)

Visible smoke of exhaust gas from iron ore sintering plants spoil the scenery around the works. In this paper, the mechanism of formation of visible smoke is discussed by detailed analysis of the exhaust gas components and dust in the Oita No. 1 sintering plant. The main substance of visible smoke was mist condensing in the air during the cooling of exhaust gas that contained 0.1 ppm of sulfuric acid. Sulfuric acid is considered to raise the dew point and so facilitate mist condensation, and to lower the vaporization of visible mist containing sulfuric acid solution. Dust in the exhaust gas is also considered to act as nucleation sites for mist condensation. This proposed mechanism was confirmed by the test remixing SOx-rich gas with desulfurization gas in the Nagoya No. 3 sintering plant. Long trails of visible smoke were observed by remixing SOx-rich gas with exhaust gas. In conclusion, in order to make exhaust gas invisible, it is not ...

Science.gov (United States)

Nitrate microelectrodes and ORP microelectrodes were fabricated to study the denitrification characteristics of dynamic membrane at different COD loadings. The denitrification process was found at 0.6-1 mm depth beneath the interface of biofilm/bulk. The results of ORP microelectrode also demonstrated that the ORP value in the range of denitrification area was between 88.6 approximately -128.4 mV which was appropriate to denitrification. When the COD loading was 0.45 kg/(m3 x d), the denitrification rate (NO3- -N) was the maximum of 0.6347 x 10(-6 mol/(L x s). With the increase of COD loading, the denitrification area was increasing and two layers with different denitrification rates emerged in the dynamic membrane. The phenomenon implied the effect of organic concentration, oxygen concentration and bacterial competition on the denitrification rate. PMID:17117632

British Library Electronic Table of Contents (United Kingdom)

Abstract Silver-impregnated wound dressings continue to be routinely used for the management of infected wounds, or wounds that are at risk of becoming infected. The ability of antimicrobials that have been incorporated into wound dressings to kill microorganisms within the dressing requires appropriate evaluation using in vitro models. In vitro models that have been exploited for this purpose have included the corrected zone of inhibition and the log reduction assay. However, these and other related culturable-based assays are purported to have poor correlation with the overall microbicidal barrier activity of an antimicrobial wound dressing. This is because culturable-based methods only retrospectively indicate bacterial cell death and do not take into account viable but nonculturable st...

British Library Electronic Table of Contents (United Kingdom)

In this work, the antibacterial effect of fluoroquinolones (FQs) upon Escherichia coli (E.coli) was measured with and without application of 40kHz ultrasound (US) stimulation. The research results demonstrated that simultaneous application of 40kHz US apparently enhanced the antibacterial effectiveness of FQs. That is, the synergistic effect was observed and the bacterial viability was reduced when FQs and US were combined. In addition, various influencing factors, such as FQs drug concentration, US irradiation time and solution temperature, on the inhibition of E.coli were also investigated. The antibacterial activity was enhanced apparently with increasing of FQs drug concentration, US irradiation time and solution temperature. Furthermore, we discussed preliminarily the mechanism of US ...

Science.gov (United States)

Some acylhydrazine derived ONO donor Schiff bases and their Co(II) and Ni(II) complexes have been prepared having the same metal ion (cation) but different anions. These synthesized metal(II) complexes have been characterized on the basis of their elemental analyses, magnetic moment, molar conductance, and IR and electronic spectral data. All of the Schiff base ligands function as tridentates and the deprotonated enolic form is preferred for coordination. In order to evaluate the effect of anions on the bactericidal activity, these synthesized complexes, in comparison to the uncomplexed Schiff bases have been screened against bacterial species., Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa and the results are reported. PMID:18475936

British Library Electronic Table of Contents (United Kingdom)

The use of indigenous bacterial root endophytes with biocontrol activity against soil-borne phytopathogens is an environmentally-friendly and ecologically-efficient action within an integrated disease management framework. The earliest steps of olive root colonization by Pseudomonas fluorescens PICF7 and Pseudomonas putida PICP2, effective biocontrol agents (BCAs) against Verticillium wilt of olive (Olea europaea L.) caused by the fungus Verticillium dahliae Kleb., are here described. A gnotobiotic study system using in vitro propagated olive plants, differential fluorescent-protein tagging of bacteria, and confocal laser scanning microscopy analysis have been successfully used to examine olive roots?Pseudomonas spp. interactions at the single-cell level. In vivo simultaneous visualization...

British Library Electronic Table of Contents (United Kingdom)

The world of antibiotic drug discovery and development is driven by the necessity to overcome antibiotic resistance in common Gram-positive and Gram-negative pathogens. However, the lack of Gram-negative activity among both recently approved antibiotics and compounds in the developmental pipeline is a general trend despite the fact that the plethora of covered drug targets are well-conserved across the bacterial kingdom. Such intrinsic resistance in Gram-negative bacteria is largely attributed to the activity of multidrug resistance (MDR) efflux pumps. Moreover, these pumps also play a significant role in acquired clinical resistance. Together, these considerations make efflux pumps attractive targets for inhibition in that the resultant efflux pump inhibitor (EPI)/antibiotic combination d...

British Library Electronic Table of Contents (United Kingdom)

Toxigenic Clostridium sordellii strains are increasingly recognized to cause highly lethal infections in humans that are typified by a toxic shock syndrome (TSS). Two glucosylating toxins, lethal toxin (TcsL) and hemorrhagic toxin (TcsH) are believed to be important in the pathogenesis of TSS. While non-toxigenic strains of C. sordellii demonstrate reduced cytotoxicity in vitro and lower virulence in animal models of infection, there are few data regarding their behavior in humans. Here we report a non-TSS C. sordellii infection in the context of a polymicrobial bacterial cholangitis. The C. sordellii strain associated with this infection did not carry either the TcsL-encoding tcsL gene or the tcsH gene for TcsH. In addition, the strain was neither cytotoxic in vitro nor lethal in a murine...

British Library Electronic Table of Contents (United Kingdom)

Complex I (NADH:ubiquinone oxidoreductase) is the largest enzyme of the mitochondrial respiratory chain. Compared to its bacterial counterpart which encompasses 14-17 subunits, mitochondrial complex I has almost tripled its subunit composition during evolution of eukaryotes, by recruitment of so-called accessory subunits, part of them being specific to distinct evolutionary lineages. The increasing availability of numerous broadly sampled eukaryotic genomes now enables the reconstruction of the evolutionary history of this large protein complex. Here, a combination of profile-based sequence comparisons and basic structural properties analyses at the protein level enabled to pinpoint homology relationships between complex I subunits from fungi, mammals or green plants, previously identified...

British Library Electronic Table of Contents (United Kingdom)

Greenman J, Thorn RMS, Saad S, Austin AJ. In vitro diffusion bed, 3-day repeat challenge `capacity' test for antimicrobial wound dressings. Int Wound J 2006;3:322-329. Abstract The aim of this study was to develop an in vitro wound infection model that allows the comparison of the bacterial kill rate of antimicrobial wound dressings over the course of 3 days, with renewed microbial challenges each day, under realistic wound-like conditions. A test bed model of a moderately exuding wound was constructed from a hydrogel containing releasable foetal calf serum (FCS), and cellulose discs dosed with test microbes (Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa) suspended in 50% FCS applied at the interface between the test dressing and the hydrogel test bed. Freshly prep...

British Library Electronic Table of Contents (United Kingdom)

In tomato crop, the induction of resistance emerges as an important alternative for achieving the reduction of chemicals in disease control. This study aimed to evaluate the ability of 28 Trichoderma isolates to promote the growth of tomato seedlings and to induce systemic resistance (ISR) against Xanthomonas euvesicatoria and Alternaria solani, the causal agents of bacterial spot and early blight, respectively. Twelve isolates promoted the increase of plant dry matter mass (DMM) above 100%, showing the great potential of these strains. All isolates were able to colonize the root system of tomato plants. The plant growth-promoting isolates were further evaluated for potential elicitation of ISR. Treatment of the soil with all Trichoderma isolates provided protection in tomato plants from 2...

British Library Electronic Table of Contents (United Kingdom)

Delayed endolymphatic hydrops (DEH) is a unique disorder characterized by fluctuating otologic symptoms in the setting of preexisting unilateral deafness. The symptoms include aural fullness, fluctuating hearing, and/or episodes of vertigo similar to those observed in Meniere disease and may occur ipsilateral or contralateral to the previously deafened ear. In most reported cases, the unilateral deafness has been a profound sensorineural hearing loss with a sudden onset that has been variously attributed to bacterial or viral labyrinthitis, acoustic or cranial trauma, otosclerosis, and congenital CMV infection. Familial occurrence of the syndrome has not previously been reported in the literature. In this report, we describe two possible familial instances of delayed DEH. These patients ra...

Energy Technology Data Exchange (ETDEWEB)

The primary charge separation in photosynthetic bacteria generates a dimeric bacteriochlorophyll (BChl) cation and a bacteriopheophytin (BPheo) anion which lie within close proximity of each other (approx. 10 A). The two radicals also lie within van der Waals contact on opposite sides of a lone BChl bridging molecule. Spectral changes in the red (Q/sub y/) band of the bridge BChls have been observed on picosecond time scales following excitation of the reaction center (RC) and have been variously attributed to the formation of a BChl anion, to a charge-transfer state, or to electrochromic effects. They present calculations here which suggest that electrochromic effects caused by the photogenerated cation and/or anion can rationalize the optical changes observed in the flash photolyses as well as in trapping experiments.

British Library Electronic Table of Contents (United Kingdom)

Ethnopharmacological relevance: Extracts of the roots of Scutellaria baicalensis Georgi (Labiatae) have been widely used to relieve fever related to bacterial infection and inflammatory diseases in traditional Korean medicine and have been reported to be effective in brain diseases. These experiments were conducted to examine the effects of oral administration of Scutellaria baicalensis extracts on the rescue of memory impairments induced by chronic cerebral hypoperfusion or chronic lipopolysaccharide (LPS) infusion. In addition, the underlying mechanisms of these effects were investigated. Materials and methods: In the first experiment, chronic cerebral hypoperfusion was induced in male Wister rats by bilateral common carotid artery occlusion (BCCAo). Daily administration of Scutellaria b...

British Library Electronic Table of Contents (United Kingdom)

Several old and new observations suggest the existence in Crohn's disease of a phagocytic disorder of macrophages related to impaired bactericidal activity of host cells or to the presence of invasive bacteria that have developed strategies to counteract macrophage killing. It was recently reported that disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease. Secretion of proinflammatory cytokines by CD macrophages was impaired in response to E. coli or specific Toll-like receptor agonists. In addition, major advances in the etiology of Crohn's disease came from the existence of polymorphism in NOD2 and autophagy-related susceptibility genes (ATG16L1 and IRGM) in patients and from the identification of the presence of adhere...

British Library Electronic Table of Contents (United Kingdom)

2,6-Diacetyl pyridine based ligand was synthesized by the reaction of 2,6-diacetyl pyridine with thiocarbohydrazide in presence of acetic acid. The coordination compounds with Cr(III) and Ni(II) metal ions having [Cr(L)X]X2 and [Ni(L)X]X compositions (where L=ligand and X=NO3^-, Cl^- and CH3COO^-) were synthesized and characterized by physicochemical and spectral studies. The studies like elemental analyses, molar conductance measurements, magnetic susceptibility measurements, IR, UV-Vis, NMR, mass and EPR reveal that the complexes are octahedral. The compounds were examined against the pathogenic fungal and bacterial strains like Alternaria brassicae, Aspergillus niger, Fusarium oxysporum, Xanthomonas compestris and Pseudomonas aeruginosa. A. niger causes the diseases Apergillosis and Oto...

British Library Electronic Table of Contents (United Kingdom)

To the best of our knowledge, this is the first report on the chemical and microbial properties of traditional Iranian fish sauce, mahyaveh. Fish sauce samples used in this study originated from five different locations in the Southern part of Iran. The pH of mahyaveh samples from different locations was in the range of 4.89-7.55 and NaCl concentration was in the range of 7.48-17.1%. The overall mean of TVB-N in all the samples tested was 3098 mg/kg. Histamine, with the overall mean of 2662 mg/kg, was found to be the main biogenic amine in the Iranian fish sauce. The high histamine content can be related to the high levels of bacterial count especially enterobacteriaceae (overall mean of 3.41 log cfu/g) and lactic acid bacteria (overall mean of 4.13 log cfu/g) in this product. Spermidine w...

British Library Electronic Table of Contents (United Kingdom)

ABSTRACT To date the effect of silver-containing wound dressings on biofilms, known to be present in chronic wounds, has not been determined or documented. In this current study, we aimed to determine the antimicrobial effect of a silver-containing dressing on biofilms grown in a chambered slide model. Before the addition of a wound dressing onto a 24-hour biofilm, composed of either Pseudomonas aeruginosa, Enterobacter cloacae, Staphylococcus aureus, or a mixed bacterial community, a fluorescent dye was applied. This enabled the viability of sessile bacteria to be monitored in real-time, using a rapid form of confocal laser scanning microscopy over a contact time period of 48 hours. By analyzing all the three-dimensional data generated from the confocal time-lapse sequences, 90% of all se...

British Library Electronic Table of Contents (United Kingdom)

Abstract:- Campylobacter jejuni-is the leading cause of bacterial diarrheal illness worldwide. Many strains are now becoming multidrug resistant. Apple-based edible films containing carvacrol and cinnamaldehyde were evaluated for bactericidal activity against antibiotic resistant and susceptible-C. jejuni-strains on chicken. Retail chicken breast samples inoculated with D28a and H2a (resistant strains) and A24a (a sensitive strain) were wrapped in apple films containing cinnamaldehyde or carvacrol at 0.5%, 1.5%, and 3% concentrations, and then incubated at 4 or 23 C for 72 h. Immediately after wrapping and at 72 h, samples were plated for enumeration of viable-C. jejuni. The antimicrobial films exhibited dose- and temperature-dependent bactericidal activity against all strains. Films with ...

Energy Technology Data Exchange (ETDEWEB)

The selection and design of an MEOR process for application in a specific field involves geological, reservoir, and biological characterization. Microbially mediated oil recovery mechanisms (bigenic gas, biopolymers, and biosurfactants) are defined by the types of microorganisms used. The engineering and biological character of a given reservoir must be understood to correctly select a microbial system to enhance oil recovery. This paper discusses the methods used to evaluate three fields with distinct characteristics and production problems for the applicability of MEOR would not be applicable in two of the three fields considered. The development of a microbial oil recovery process for the third field appeared promising. Development of a bacterial consortium capable of producing the desired metabolites was initiated, and field isolates were characterized.

British Library Electronic Table of Contents (United Kingdom)

This paper describes a comprehensive model of wastewater treatment in secondary facultative ponds, which combines 3D hydrodynamics with a mechanistic water quality model. The hydrodynamics are based on the Navier-Stokes equation for incompressible fluids under shallow water and Boussinesq assumptions capturing the flow dynamics along length, breadth and depth of the pond. The water quality sub model is based on the Activated Sludge Model (ASM) concept, describing COD and nutrient removal as function of bacterial growth following Monod kinetics, except for Escherichia coli removal, which was modelled as first order decay. The model was implemented in the Delft3D software and was used to evaluate the effect of wind and the addition of baffles on the water flow pattern, temperature profiles i...

International Nuclear Information System (INIS)

A study of stress corrosion cracking susceptibility of Zircaloy-4 in chloride-containing aqueous methanolic media is presented. The influence of water content is investigated in the 5-100 vol. % water range. The dependence of stress corrosion cracking on potential is evidenced. A close correlation is established with pitting susceptibility determined by a statistical analysis of induction times. A correlation with the repassivation potential is observed in the water-rich solutions. In the low water content solutions, two repassivation curves are determined according to the experimental technique. Pit morphology and fractography show a transition from aqueous to organic media. (author).

International Nuclear Information System (INIS)

SP-700, an emerging #beta#-rich #alpha#+#beta# titanium alloy, is designed to improve superplastic formability as well as mechanical properties over Ti-6Al-4V alloy. Owing to its fine microstructure and low #beta#-transus temperature, it is superplastic-formable at temperature below 1,073K (800 C) with low flow stress. Remarkable workability of this alloy is also retained in conventional Manufacturing processes. Another advantage of SP-700 is heat treatment response which includes deep hardenability and quick aging kinetics. Corrosion resistance and machinability are equivalent to or better than Ti-6Al-4V alloy.

Energy Technology Data Exchange (ETDEWEB)

British Columbia is rich in resources of coal but is deficient in resources of liquid petroleum. The overall objective of the B.C. Research Coal Liquefaction Program is to provide sufficient technical data to identify and evaluate coal conversion opportunities in the province. In this first quarterly report, liquefaction test systems are discussed and results from the testing of equipment, procedures and analytical methods using coal samples from Sukunka (N.E. coal) and Hat Creek (central interior) are presented. The proposed program to be undertaken over the next four years is outlined.

International Nuclear Information System (INIS)

The masses of 16 neutron-rich nuclei in the mass range from 35 to 45 have been measured using a direct time of flight technique following the fragmentation of a "4"8Ca beam at 60 MeV/nucleon. The masses of "3"5","3"6Mg, "3"8Al, "3"9","4"0Si, "4"2","4"3P and "4"3","4"4S are reported for the first time. Preliminary analysis shows that the N=28 shell closure persists, even if weakened by the large neutron excess.

Energy Technology Data Exchange (ETDEWEB)

The performance of a high temperature ion source coupled to a helium gas-jet transport system for an efficient mass separation of neutron-rich alkaline earth and lanthanide isotopes is reported and the results of overall efficiency measurements using different cluster materials in the gas-jet are given. A fast, microprocessor controlled tape transport system for ..gamma..-spectroscopic studies on short-lived isotopes is described. Some results on the decay of 3.8sub(-s) /sup 152/Pr are presented.

Science.gov (United States)

A plethora of experimental studies use mtDNA as a marker of demographic processes without questioning the possibility that selection may bias their interpretations. We studied four lines of Drosophila melanogaster that have a standardized nuclear DNA but variable mtDNA. We completed the sequencing of the mitochondrial genomes (excluding the A+T rich region) and compiled the differences. We then assayed male influence on oviposition, starvation resistance, lipid proportion and physical activity. We discuss these results in terms of the known differences between the lines and conclude that naturally occurring mtDNA variants in D. melanogaster are expressed at the level of the organismal phenotype. PMID:21757031

CERN Document Server

We highlight the role of the light elements (Li, Be, B) in the evolution of massive single and binary stars, which is largely restricted to a diagnostic value, and foremost so for the element boron. However, we show that the boron surface abundance in massive early type stars contains key information about their foregoing evolution which is not obtainable otherwise. In particular, it allows to constrain internal mixing processes and potential previous mass transfer event for binary stars (even if the companion has disappeared). It may also help solving the mystery of the slowly rotating nitrogen-rich massive main sequence stars.

Wastenet

...Investigating Biochar: from Source to Sink (Overview - Research) The Sustainable Agriculture Flagship is leading national collaborative research analysing the properties ...and potential of a variety of biochars to improve soil health and sequester carbon. The Sustainable Agriculture Flagship is leading national collaborative research ...analysing the properties and potential of a variety of biochars to improve soil health and sequester carbon. Biochar, sustainable agriculture, soil,...carbon, SAF, pyrolysis, fertiliser, biochar, soil types, greenhouse gas, herbicide, pesticide, bioenergy, soil science, carbon-rich solid, ...

International Nuclear Information System (INIS)

We present the efficient technique to extract the signal of the intermediate mass Higgs boson from the backgrounds at future #gamma##gamma# colliders. For a clear Higgs detection, it is important to fit the original electron accelerator energy depending on the Higgs mass, to set the polarization of the photon beams and to apply the efficient b quark tagging method. we demonstrate the extraction of information of Higgs parameters and the new physics from the observable physical quantities. It is clearly shown that a future #gamma##gamma# collider will have a rich potential for study on the new physics, as well as the Higgs physics. (author).

International Nuclear Information System (INIS)

We have investigated the formation and coarsening of Ga droplets on focused-ion-beam (FIB) irradiated GaAs surfaces. To separately examine formation and coarsening, Ga droplets were fabricated by Ga"+ FIB irradiation of GaAs substrates with and without pre-patterned holes. We determined the droplet growth rate and size distribution as a function of FIB energy following irradiation. The data suggest a droplet formation mechanism that involves Ga precipitation from a Ga-rich layer, followed by droplet coarsening via a combination of diffusion and Ostwald ripening or coalescence via droplet migration (dynamic coalescence).

International Nuclear Information System (INIS)

It is experimentally demonstrated that a relatively strong ion-rich sheath formed at a fixed negative bias of the grid can be changed to a rather weak ion sheath (sheath potential weakly retards electrons) only by increasing the discharge voltage in the system. At sufficiently high negative grid bias, an increase of discharge voltage enhances the ion collection current at the grid. An explanation is put forward in support of this experimental observation. A slight density enhancement with a fall in plasma electron temperature is also observed with the increasing negative grid bias. (authors)

CERN Document Server

Developing in Ext GWT is a fast--paced, practical guide to quickly learning the tasks necessary in building enterprise--class Rich Internet Applications. Based around the exciting new UI library from Ajax leaders Ext JS and the latest GWT release, this book takes the reader through setup, the available widgets, and advanced custom widgets and templates, and concludes with a functional sample client--server application in less than 150 pages. Not your typical beginner's guide to programming, this book provides a rapid approach to becoming effective with leading commercial RIA tools and librarie

Energy Technology Data Exchange (ETDEWEB)

The energy-integrated reaction cross-sections of several neutron-rich nuclei ({sup 17-22}N, {sup 19-24}O, {sup 21-27}F, {sup 23-30}Ne, {sup 26-33}Na, {sup 28-35}Mg, {sup 31-38}Al, {sup 33-40}Si, {sup 36-42}P, {sup 39-44}S, {sup 42-45}Cl, {sup 45,46}Ar), measured at intermediate energy (30-65 A-bar MeV), via direct method, are presented. Silicon detectors have been used as the active target as well as for particles identification. The reduced strong absorption radii r{sub 0}{sup 2} are extracted and compared to the data available from the literature. New measurements for 19 nuclei ({sup 27}F, {sup 27,30}Ne, {sup 33}Na, {sup 28,34-35}Mg, {sup 36-38}Al, {sup 38-40}Si, {sup 41-42}P, {sup 42-44}S, {sup 45}Cl) are revealed. From the study of the isospin dependence of the reduced strong absorption radius, a new quadratic parameterisation of the nuclear radii in the closed shell regions N=8 and N=28, is proposed. According to this parameterisation, the proton/neutron ...

International Nuclear Information System (INIS)

Arsenic was predominantly present as inorganic arsenite in the fronds of the hyperaccumulator Chinese brake. - Arsenic speciation is important not only for understanding the mechanisms of arsenic accumulation and detoxification by hyperaccumulators, but also for designing disposal options of arsenic-rich biomass. The primary objective of this research was to understand the speciation and leachability of arsenic in the fronds of Chinese brake (Pteris vittata L.), an arsenic hyperaccumulator, with an emphasis on the implications for arsenic-rich biomass disposal. Chinese brake was grown for 18 weeks in a soil spiked with 50 mg As kg"-"1 as arsenate (AsO_4"3"-), arsenite (AsO_3"3"-), dimethylarsinic acid (DMA), or methylarsonic acid (MMA). Plant samples were extracted with methanol/water (1:1) and arsenic speciation was performed using high performance liquid chromatography coupled with atomic fluorescence spectrometry. The impacts of air-drying ...

DEFF Research Database (Denmark)

Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage proteins and amino acid composition. The antisense line had a lower C-hordein level and down-regulated transcript encoding C-hordein. The production of the S-rich B/ - and D-hordeins ...