WorldWideScience
1

Final Report on the Evaluation of the NSF's Instructional Materials Development Program  

Science.gov (United States)

... of the Evaluation of the Instructional Materials Development Program (PDF, 412 kb) Chapter I ... kb) Appendix 2: Instrument for Evaluating Materials (PDF, 935 kb) Appendix 3: Classroom Observation ...

2

Growth regulation of Legionella Pneumophila in biofilms and amoebae; Wachstumsregulation von Legionella Pneumophila in Biofilmen und Amoeben  

Energy Technology Data Exchange (ETDEWEB)

This final report for the Swiss Federal Office of Energy (SFOE) presents the results of studies made on the regulation of the growth of Legionella Pneumophila bacteria in biofilms and amoebae. In a first project, the formation of biofilms by Legionella Pneumophila bacteria was analysed in static and dynamic systems using a complex growth medium. Under static and dynamic clinical and environmental conditions, the adherence of the biofilms on polystyrene tissue was studied. This was also examined under dynamic flow conditions. In a second part of the project, the regulation of growth of Legionella Pneumophila in amoebae was examined in that changes were made to the genome of the bacteria. The importance of the work for the de-activation of Legionella Pneumophila bacteria in biofilms is noted in the conclusions of the report.

2006-07-01

3

Molecular biological enhancement of coal biodesulfurization. Final report, October 1988--December 1991  

Energy Technology Data Exchange (ETDEWEB)

The overall objective of this project was to use molecular genetics to develop strains of bacteria with enhanced ability to remove sulfur from coal, and to obtain data that will allow the performance and economics of a coal biodesulfurization process to be predicted. (VC)

1991-12-01

4

Appendix W: deep sea biophysics (microbiology and amphiped studies)  

International Nuclear Information System (INIS)

CNPT-3 is the laboratory designation of a strain of bacteria that reproduces best at deep-sea pressures. It was isolated from a sample collected at a 5800 m depth of the Pacific Ocean. This isolation was achieved from a sample that had been retrieved without warning. This year we demonstrated that there are similarly behaving bacteria that can be isolated from samples even when retrieved with decompression. The purpose of this project was to define the temperatures and pressures over which CNPT-3 can exist and function. (1) At 2"0C CNPT-3 is eurybathic from about 1000 to 6500 m, as judged from an only slightly pressure dependent generation time. (2) CNPT-3 is unquestionably barophilic at 2"0C, with doubling times of 9 hours at 580 bars and over 18 hours at 1 bar. (3) At 10"0C, reproduction is adversely affected at 1 bar to the extent that CNPT-3 becomes obligately barophilic at 10"0C. Yet reproduction is enhanced above 280 bars and occurs with ...

1981-04-01

5

Simplified solar fraction estimation for space and water heating at dod installations. appendix c. water heating nomographs. Final report  

Energy Technology Data Exchange (ETDEWEB)

This report contains a set of nomographs which can be used to estimate the average annual solar fraction for solar space and water heating at a large number of DOD facilities. The solar fraction estimated from the nomograph is in close agreement with F-Chart 3.0 and allows for variation of the following parameters: annual load, collector area, collector transmittance-absorptance coefficient, and collector overall loss coefficient.

1982-09-01

6

Simplified solar fraction estimation for space and water heating at DOD installations. appendix b. space heating nomographs. Final report  

Energy Technology Data Exchange (ETDEWEB)

A set of nomographs is presented which can be used to estimate the average annual solar fraction for solar space and water heating at a large number of DOD facilities. The solar fraction estimated from the nomograph is in close agreement with F-Chart 3.0 and allows for variation of the following parameters: annual load, collector area, collector transmittance-absorptance coefficient, and collector overall loss coefficient.

1982-09-01

7

Satellite power systems (SPS) concept definition study (Exhibit D). Volume 4, Part 2: cost and programmatics appendix. Final report  

Energy Technology Data Exchange (ETDEWEB)

Cost and programmatic aspects of a recommended satellite power system are documented. Computer generated summaries are presented, and the detailed computer runs structured in a Work Breakdown Structure are given. The six configurations developed during the study period are summarized.

1981-03-01

8

Remedial action plan and site design for stabilization of the inactive uranium mill tailings sites at Rifle, Colorado. Volume 5, Addenda D6--D8 to Appendix D: Final report  

Energy Technology Data Exchange (ETDEWEB)

This volume contains appendices D6 through D8 containing laboratory test data: from MK-F investigation, 1987, Old Rifle and New Rifle sites; on bentonite amended radon barrier material; and from MK-F investigation, 1987, riprap tests.

1990-02-01

9

White River Falls Fish Passage Project, Tygh Valley, Oregon : Final Technical Report, Volume III, Appendix B, Fisheries Report; Appendix C, Engineering Alternative Evaluation; Appendix D, Benefit/Cost Analysis.  

Energy Technology Data Exchange (ETDEWEB)

Studies were conducted to describe current habitat conditions in the White River basin above White River Falls and to evaluate the potential to produce anadromous fish. An inventory of spawning and rearing habitats, irrigation diversions, and enhancement opportunities for anadromous fish in the White River drainage was conducted. Survival of juvenile fish at White River Falls was estimated by releasing juvenile chinook and steelhead above the falls during high and low flow periods and recapturing them below the falls in 1983 and 1984. Four alternatives to provide upstream passage for adult salmon and steelhead were developd to a predesign level. The cost of adult passage and the estimated run size of anadromous fish were used to determine the benefit/cost of the preferred alternative. Possible effects of the introduction of anadromous fish on resident fish and on nearby Oak Springs Hatchery were evaluated. This included an inventory of resident species, a genetic study of native ...

1985-06-01

10

Safeguards and Security Technology Development Directory. FY 1993  

Energy Technology Data Exchange (ETDEWEB)

The Safeguards and Security Technology Development Directory is published annually by the Office of Safeguards and Security (OSS) of the US Department of Energy (DOE), and is Intended to inform recipients of the full scope of the OSS R&D program. It is distributed for use by DOE headquarters personnel, DOE program offices, DOE field offices, DOE operating contractors, national laboratories, other federal agencies, and foreign governments. Chapters 1 through 7 of the Directory provide general information regarding the Technology Development Program, including the mission, program description, organizational roles and responsibilities, technology development lifecycle, requirements analysis, program formulation, the task selection process, technology development infrastructure, technology transfer activities, and current research and development tasks. These chapters are followed by a series of appendices which contain more specific information on aspects of the Program. ...

1993-06-01

11

Probabilistic endowment appraisal system based upon the formalization of geologic decisions. National Uranium Resource Evaluation. Final report: demonstration and comparative analysis of estimates and methods  

Energy Technology Data Exchange (ETDEWEB)

This report is the culmination of approximately three and one-half years of research on the design and demonstration of a mineral endowment appraisal system which has as its foundation a formalization of geological decisions within a probabilistic framework. Basically, the probabilistic decision structure of a geologist or team of geologists is captured in a system of computer programs which can be used by a geologist for the estimation of the magnitude of uranium endowment by providing information to this system about the states of earth processes and geologic conditions that pertain to that region being evaluated. Research performed under this contract had two main objectives: demonstration of the endowment appraisal system on the San Juan Basin of New Mexico; a comparison of estimates by the appraisal system with estimates made using other methodologies. The scope of this report is restricted to a demonstration of the appraisal system and the comparison of system estimates for ...

1980-12-01

12

APPENDIX-GLOSSARY - MSFC History Office - NASA  

Science.gov (United States)

Exploding Bridgewire . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. Fusion Weld . ...

13

45 CFR Appendix A to Part 611 - Appendix A to Part 611  

Science.gov (United States)

45 Public Welfare 3 2010-10-01 2010-10-01 false Appendix A to Part 611 A Appendix A to Part 611 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE...

2010-10-01

14

Microbial indicators of environmental water pollution. Mizu kankyo ni okeru biseibutsu shihyo  

Energy Technology Data Exchange (ETDEWEB)

Microbial indicators in the environmental water are categorized as follows. 1. Index for pathogenic microbial contamination, especially enteric canal based ones. 2. Index for the ordinary microbial contamination. 3. Index for nutritive assessment. 4. Index for the treating effect of final effluent discharged into an environmental water. 5. Index for distinction of of the contamination source. Above 1 and 2'' are among the indices which cannot be replaced by other physico-chemical substances or indices. Up to the present time, 1'' is assessed by a coliform group and 2'' by the bacteria. Recently, however, the status on the microbial contamination of the environmental water is changing, thus urging the reexamination of the assessment systems. This report describes the following items. Characteristics and the elements of the indices (enteric canal based pathogenic microbial contamination); ...

1990-08-10

15

Synthesis of model compounds for coal liquefaction research: Final report, June 21, 1990--July 30, 1993  

Energy Technology Data Exchange (ETDEWEB)

The objectives of this project were to develop feasible synthetic routes to produce (1) 4(4{prime}-hydroxy-5{prime}6{prime},7{prime}8{prime}-tetrahydro-1{prime}-naphthylmethyl)-6-methyldibenzothiophene, and (2) a 1-hydroxynaphthalene-dibenzothiophene polymer. These compounds are thought to be representative of sulfur containing molecules in coal. The program was divided into two technical tasks. Unfortunately, the attempted syntheses of these compounds was unsuccessful due to their unusual reactivities. Attempted synthetic routes and possible future routes are described, and Appendix A lists the compounds that were synthesized during this program.

1993-07-01

16

An energy management teaching module for management education in the Higher Vocational Education schools in the Netherlands. Lesmodule energiebeheer voor managementgericht onderwijs in het Hoger Beroepsonderwijs; Fase 1: Market research (Final report)  

Energy Technology Data Exchange (ETDEWEB)

The demand for energy management in teaching packages of Higher Vocational schools in the Netherlands and the possibilities to implement energy management into the different sectors of this school type have been investigated. The results are based on interviews with representative of the above-mentioned school types. Recommendations are made for a follow-up of this investigation in order to set up an energy management teaching module. 1 tab., 1 appendix

1992-02-01

17

Photovoltaic industry manufacturing technology. Final report  

Energy Technology Data Exchange (ETDEWEB)

This report contains the results of the Photovoltaic (PV) Industry Manufacturing Technology Assessment performed by the Automation and Robotics Research Institute (ARRI) of the University of Texas at Arlington for the National Renewable Energy laboratory. ARRI surveyed eleven companies to determine their state-of-manufacturing in the areas of engineering design, operations management, manufacturing technology, equipment maintenance, quality management, and plant conditions. Interviews with company personnel and plant tours at each of the facilities were conducted and the information compiled. The report is divided into two main segments. The first part of the report presents how the industry as a whole conforms to ``World Class`` manufacturing practices. Conclusions are drawn from the results of a survey as to the areas that the PV industry can improve on to become more competitive in the industry and World Class. Appendix A contains the questions asked in the ...

1998-08-01

18

Mine drainage treatment; Kogyo haisui shori  

Energy Technology Data Exchange (ETDEWEB)

The generation of acidic mine drainage is based on the oxidation dissolution of sulfide minerals due to the contact of ores with the natural water and the air. Namely, the sulfuric acid promotes the dissolution of sulfide minerals and gangue minerals, and Fe{sup 2+} is further oxidized to turn into Fe{sup 3+}, which acts as an oxidizer for other sulfide minerals and promotes the dissolution of the same. This paper roughly describes a mine drainage treatment focusing on an Fe{sup 2+} neutralization method and an iron bacteria oxidation method. The iron bacteria oxidation method is a method of oxidizing Fe{sup 2+} into Fe{sup 3+} by using iron bacteria, and then removing Fe{sup 3+} with calcium carbonate. This method has been used for treating the drainage in the Sakuhara Mine and the former Matsuo Mine, with good results. The paper finally introduces, as power-saving low-cost mine drainage treatment ...

1996-05-25

19

I. Introduction  

Science.gov (United States)

... Experts ( Appendix 5 ), and the NMFS-Sea Grant Joint Graduate Fellowship Program ( Appendix 6) . The Task Force consisted of one representative from NMFS Headquarters and 1-2 rep...

20

Corps of Engineers Structural Engineering Conference Held ...  

Science.gov (United States)

... CI Appendix D: Conference Attendees ..... D1 Appendix E: Structural Steel Connection Design on Federal Projects ..... El iv ...

1992-12-01

23

Site Operator technical report. Final report (1992--1996)  

Energy Technology Data Exchange (ETDEWEB)

The Southern California Edison Company (SCE) and the US Department of Energy (DOE) entered into cooperative agreement No. DE-FC07-91ID13077 on August 23, 1991, which expired on August 3, 1996. This cooperative agreement provided SCE with DOE cofunding for participation in the DOE`s Electric and Hybrid Vehicle Site Operator Program. In return, SCE provided the DOE with quarterly progress reports which include operating and maintenance data for the electric (EVs) vehicles in SCE`s fleet. Herein is SCE`s final report for the 1992 to 1996 agreement period. As of September 1, 1996 the SCE fleet had 65 electric vehicles in service. A total of 578,200 miles had been logged. During the agreement period, SCE sent the DOE a total of 19 technical reports (Appendix B). This report summarizes the technical achievements which took place during a long, productive and rewarding, relationship with the DOE.

1996-12-01

24

Evaluation of measuring data of small photovoltaic power systems within the framework of the German Federal-State 1000 Roofs PV programme. Final report; 1000-Daecher Mess- und Auswerteprogramm. Auswertung der Messdaten von photovoltaischen Kleinanlagen im Rahmen des Bund-Laender-1000 Daecher-Photovoltaik-Programms. Wissenschaftlicher Endbericht  

Energy Technology Data Exchange (ETDEWEB)

Within the framework of the German Federal-State 1000 Roofs Photovoltaic Programme monthly measuring data and verbal comments on the PV system operation (reports on failures and defects) have been collected, stored in a databank and evaluated for the period 1991 to 1997 for a differing number of PV systems. In addition to this came along data of initially 100, then 40 intensively measured systems. The results of the measuring data evaluation as well as the results of further accompanying research projects to the Federal-State 1000 Roofs Photovoltaic Programme (e.g. low energy yield analysis, on-site inspections and measuring of nominal powers) are summarised. Starting from the gained results, conclusions and recommendations are given for the design, installation and operation for future grid-connected PV-systems. Also it is pointed to the remaining need for optimisation and R and D. Additional part of the report are the Annual Journal (published in a large print run) and the ...

1998-10-16

25

Development document for proposed effluent limitations guidelines and standards for the centralized waste treatment industry. Volumes 1 and 2  

Energy Technology Data Exchange (ETDEWEB)

This document includes the following topics: Background; Data Collection; Scope/Applicability of the Proposed Regulation; Description of the Industry; Industry Subcategorization; Pollutants of Concern for the Centralized Waste Treatment Industry; Pollutants Selected for Regulation; Wastewater Treatment Technologies; Regulatory Options Considered and Selected for Basis of Regulation; Long-Term Averages, Variability Factors, and Limitations and Standards; Cost of Treatment Technologies; Pollutant Loading and Removal Estimates; Non-Water Quality Impacts; Implementation; Analytical Methods and Baseline Values; List of Definitions; List of Acronyms; Appendix A--Pollutant Groups; Appendix B--Listing of Characerization Data From Non-Hazardous Oils Facilities; Appendix C--Listing of Daily Influent and Effluent Measuements; Appendix D--Facility-Specific Compliance Costs; Appendix ...

1998-12-01

26

Nonphotochemical Hole-Burning Studies of Energy Transfer Dynamics in Antenna Complexes of Photosynthetic Bacteria  

Energy Technology Data Exchange (ETDEWEB)

This thesis contains the candidate's original work on excitonic structure and energy transfer dynamics of two bacterial antenna complexes as studied using spectral hole-burning spectroscopy. The general introduction is divided into two chapters (1 and 2). Chapter 1 provides background material on photosynthesis and bacterial antenna complexes with emphasis on the two bacterial antenna systems related to the thesis research. Chapter 2 reviews the underlying principles and mechanism of persistent nonphotochemical hole-burning (NPHB) spectroscopy. Relevant energy transfer theories are also discussed. Chapters 3 and 4 are papers by the candidate that have been published. Chapter 3 describes the application of NPHB spectroscopy to the Fenna-Matthews-Olson (FMO) complex from the green sulfur bacterium Prosthecochloris aestuarii; emphasis is on determination of the low energy vibrational structure that is important for understanding the energy transfer process associated within three ...

2002-06-27

27

Economic analysis of proposed effluent limitations guidelines and standards for synthetic-based drilling fluids and other non-aqueous drilling fluids in the oil and gas extraction point source category  

Science.gov (United States)

The Economic Analysis (EA) report is written to address the impacts of this proposed Effluent Limitation Guidelines and Standards for Synthetic-Based and Other Non-Aqueous Drilling Fluids. Currently, effluent guidelines pertaining to the discharge of drilling fluids address two specific types of fluids: Oil-based drilling fluids (OBFs) that use diesel and mineral oil, which are prohibited from being discharged; and Water-based drilling Fluids (WBFs), which can be discharged subject to meeting certain discharge requirements, including a sheen test and an aqueous toxicity test, in certain limited offshore regions. Section Two presents sources of data, Section Three presents the industry profile, Section Four discusses the regulatory costs of options under consideration for the proposed rulemaking, and Section Five discusses the impacts of the proposed rule on firms, well drilling, and production, and also briefly discusses secondary impacts such as those on employment, output, inflation, ...

1999-02-01

28

Sound climate installations. Gezonde klimaatinstallaties  

Energy Technology Data Exchange (ETDEWEB)

An uncomfortable thermal climate, draft complaints and a bad airquality are often related to the climate installation. To avoid badfunctioning of the system a number of preconditions has to be fulfilledwith regard to design, planning and execution, adjustments, completion,control, maintenance and management of the installations. First threetypes of health problems in buildings are shortly discussed: buildingrelated illness, sick building syndrome and building or installationrelated complaints. Then some functions of the climate installationsare described: air filtration, filter classes and filter quality, aswell as investment costs for better filters. Next the causes for thecomplaints are dealt with: air conditioning, ventilation, air quality,and temperatures. Subsequently health affecting aspects in relation tothe climate systems are discussed: outdoor air pollution,microorganisms, bacteria (legionella pneumophila), allergies, ionneutralization and performance of ...

1989-10-01

29

Effects of fungicides on endophytic fungi and photosynthesis in seedlings of a tropical tree, guarea guidonia (meliaceae)  

International Nuclear Information System (INIS)

Endophytes are microorganisms that live within healthy plant tissues, and include fungi and bacteria. They can be mutualists, comensals or even latent pathogens. Presence of these endosymbionts may affect host physiology, for example by consuming products of photosynthesis (endophytes are heterotrophs) or producing toxic metabolites. In this work two fungicides were used to eliminate fungal endophytes from seedlings of guarea guidonia. light saturated photosynthesis (Amax) was measured in endophytefree plants and compared with control plants. Each fungicide killed different fungal endosymbionts. phomopsis was more susceptible to benomyl while colletotrichum was more susceptible to propiconazole. Although suggestive, values of Amax were not significantly different for each treatment compared with control plants. No prediction can be made at this point about the final outcome of a given plantendophytic fungi interaction

2005-12-01

30

Production Data Package 267 Gallon External Fuel Tank  

Science.gov (United States)

... It is recommended that the drawings be validated in accordance with NAVAIRINST 4333.10 and the data package, Appendix A, be used for ...

1973-08-23

31

Flue Gas Desulfurization and Low Btu Gasification - A ...  

Science.gov (United States)

... Accession Number : ADD419503. Title : Flue Gas Desulfurization and Low Btu Gasification - A Comparison Appendix G. ...

1975-05-01

32

An Analysis of Small Navy Tactics Using a Modified Hughes' ...  

Science.gov (United States)

... APPENDIX A: A PROPOSED STAYING POWER MODEL .....73 A. INTRODUCTION .....73 B. PROPOSED STAYING ...

2007-03-01

33

Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria  

UK PubMed Central (United Kingdom)

The cell wall envelopes of gram-positive bacteria represent a surface organelle that not only functions as a cytoskeletal element but also promotes interactions between bacteria and their environment....Full Text Available

2006-03-01

34

Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria.  

UK PubMed Central (United Kingdom)

Three nucleic acid probes, two for autotrophic ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria and one for alpha subdivision nitrite-oxidizing bacteria, were developed...Full Text Available

1996-08-01

35

User`s guide and documentation manual for microbial transport simulator  

Energy Technology Data Exchange (ETDEWEB)

The microbial transport simulator (MTS) is a three-dimensional, three-phase, multiple-component numerical model that permits the study of the transport of microorganisms and nutrients in porous media. Microbial parameters incorporated into MTS include: Microbial growth and decay, microbial deposition, chemotaxis, diffusion, convective dispersion, tumbling, and nutrient consumption. Governing equations for microbial and nutrient transport are coupled with continuity and flow equations under conditions appropriate for a black oil reservoir. The model`s mathematical formulations and preparation procedures of data files for conducting simulations using MTS are described. A general background of microbial transport simulation is given in Section I and the governing equations, mechanisms, and numerical solutions of MTS are given in Section II. Explanations for preparing an input data file with reservoir and microbial transport data are described in Section III, and recurrent data are given ...

1993-10-01

36

Increase in the number of distributed power generation installations in electricity distribution grids - Project findings; Zunahme der dezentralen Energieerzeugungsanlagen in elektrischen Verteilnetzen: Erkenntnisse aus dem Projekt  

Energy Technology Data Exchange (ETDEWEB)

This is the eighth part of a ten-part final report for the Swiss Federal Office of Energy (SFOE) on a project that looked into potential problems relating to the Swiss electricity distribution grid with respect to the increasing number of distributed power generation facilities being put into service. The identification of special conditions for the grid's operation and future development that take increasing decentralised power production into account are discussed. The results of the project activities encompass the analysis and evaluation of various problem areas associated with planning and management of the grid during normal operation and periods of malfunction, as well as required modifications to safety systems and grid configurations. This seventh appendix to the main report summarises and discusses the knowledge gained during the project and attempts to provide answers to various questions posed by the use of distributed ...

2003-07-01

37

Effect of different nitroheterocyclic compounds on aerobic, microaerophilic, and anaerobic bacteria.  

UK PubMed Central (United Kingdom)

The antibacterial activities of different nitroheterocyclic compounds were assessed by an agar dilution method against aerobic, microaerophilic, and anaerobic bacteria. Nitronaphthofurans inhibited...Full Text Available

1986-11-01

38

Demand 80/81: forecasts of energy consumption to the year 2000. Volume 2: appendixes. Final report  

Energy Technology Data Exchange (ETDEWEB)

The Demand 80/81 forecasts cover each sector of the economy and are conditional upon alternative assumptions concerning national economic growth, energy prices, other prices, and conservation policy. The forecasts also include forms of energy other than electricity to provide a comprehensive energy picture. Although the forecasts proceed through time in one-year increments, their primary focus is on the period from 1985 to 2000. The forecasts are based on twelve sets of alternative assumptions, or scenarios. A forecast of economic growth and nonenergy variables is used to drive the model to 12 different conditional forecasts. These represent four sets of price assumptions and three sets of non-price conservation assumptions. The price assumptions are: high, baseline, low and constant 1976, which - because of the inverse relationship between prices and consumption - represent low consumption, baseline consumption, high consumption, and extra high consumption, respectively. The non-price ...

1981-10-01

40

MANAGEMENT PRE-START REVIEW FINAL REPORT FOR THE BIOSAFETY LEVEL 3 (BSL-3) FACILITY (B368) LAWRENCE LIVERMORE NATIONAL LABORATORY  

Energy Technology Data Exchange (ETDEWEB)

A Lawrence Livermore National Laboratory (LLNL) Management Pre-Start Review (MPR) Team was formed to independently verify the operational readiness of Building 368 (B368) Biosafety Level III (BSL-3) Facility to conduct research with biological pathogens and toxins including those considered Select Agents. Review objectives and criteria were developed from the DOE/NNSA and LLNL requirements. These were provided in the Implementation Plan for the Biosafety Level III (BSL-3) Facility Management Pre-Start Review (BSL-3 MPR) at Lawrence Livermore National Laboratory that was reviewed and approved by DOE/NNSA-LSO. The formal part of the LLNL MPR for the BSL-3 Facility was begun in August of 2005 but work on the MPR was stopped in October of 2005 due to the need for LLNL to reassess organizational and operational controls and respond to Centers for Disease Control and Prevention inquiries related to a shipping incident involving select agents. The MPR was restarted in mid-June of 2006. ...

2006-07-25

41

Use of BWR/6 PRA to assess the effect of proposed new QA requirements  

International Nuclear Information System (INIS)

The probabilistic risk assessment (PRA) of the General Electric BWR/6 Mark III standard plant has been used to assess the effect of proposed new quality assurance requirements on the off-site plant risk. This work was performed by General Electric Company under contract to the Electric Power Research Institute. First, the potential changes in the reliability of structures, systems and components were estimated. Then those changes that could affect the event tree and fault tree results were identified. Finally these changes were propagated through the probabilistic risk assessment fault trees and event trees to determine the effect upon off-site plant risk. The results show that applying quality assurance requirements, very nearly equal to those of 10CFR50, Appendix B (3), to nearly all nonsafety-related structures, systems, and components would have a negligible effect on off-site plant risk. In this paper, first the proposed new quality ...

1985-02-01

42

Hawaii demand-side management resource assessment. Final report, Reference Volume 1: Building prototype analysis  

Energy Technology Data Exchange (ETDEWEB)

This report provides a detailed description of, and the baseline assumptions and simulation results for, the building prototype simulations conducted for the building types designated in the Work Plan for Demand-side Management Assessment of Hawaii`s Demand-Side Resources (HES-4, Phase 2). This report represents the second revision to the initial building prototype description report provided to DBEDT early in the project. Modifications and revisions to the prototypes, based on further calibration efforts and on comments received from DBEDT Staff have been incorporated into this final version. These baseline prototypes form the basis upon which the DSM measure impact estimates and the DSM measure data base were developed for this project. This report presents detailed information for each of the 17 different building prototypes developed for use with the DOE-21E program (23 buildings in total, including resorts and hotels defined separately for each island) to ...

1995-04-01

43

Biosorption of heavy metals under anaerobic conditions. Final report; Biosorption von Schwermetallen unter anaeroben Bedingungen. Abschlussbericht  

Energy Technology Data Exchange (ETDEWEB)

The precipitation of heavy metals as hydroxides is the standard technique for the decontamination of waste water streams polluted by these elements. On the other side, progress in research has been made concerning the biosorption onto dead biomass and bioprecipitation supported by physiologically active bacteria. As the aim of this study, a flexible strategy has been envisaged cleaning a waste water with definite heavy metal load underlying the process mentioned above. Suitable bacteria were enriched and the process was tested in a technical plant. As result, a very high efficiency of heavy metal elimination has been found. The field of application covered by the acquired process is identical with the whole range of the waste water streams polluted by heavy metals. In addition, a second stage may be necessary if there are any further contaminants to be removed. (orig.) [Deutsch] Bei der Reinigung von schwermetallhaltigen Abwaessern ist der ...

1996-12-31

44

BIBRA "t"r"a"d"e"m"a"r"k - the biological treatment of radioactive waste water  

International Nuclear Information System (INIS)

BIBRA "t"r"a"d"e"m"a"r"k, is the new bio-technological method developed in Gundremmingen for treating radioactive waste water, using bacteria in a process analogous to the long-established principle of communal sewage treatment plants. The method exploits the behaviour of the micro-organisms found there, to establish optimum adaptation of their population for decomposing the typical pollutants found in this washing water. This procedure is particularly suitable for nuclear engineering plants, because in such plants the waste water composition changes little so that the bacteria can achieve optimum adaptation to this waste water. The organic ingredients of the washing media are decomposed by introducing air. The advantage of the procedure is not only the significant reduction of the amount of waste material, but also enhanced efficiency of the cleaning process. The decontamination factor in Gundremmingen improved from a factor of 5 to a factor ...

1999-03-01

45

Syntrophic microorganisms in anaerobic environments. Zettai kenki kankyoka ni okeru biseibutsu no kyosei  

Energy Technology Data Exchange (ETDEWEB)

The most classic information on the syntrophic microorganisms in an anaerobic bacteria symbiotic system may be methanobacillus omelianskii. These bacteria were isolated as ones degrading alcohols such as ethanol into methane and were stably preserved for more than 20 years. But, it was clarified by later researches that this was a kind of symbiotic cocultivation system of the bacteria (S strain) which oxidizes ethanol into acetic acid, hydrogen, and carbon dioxide, and a kind of bacteria of the methanobacterium genus which produces or synthesizes methane from hydrogen and carbon dioxide. Such a symbiotic system of two kinds of anaerobic bacteria is the one of a kind of bacteria which produces hydrogen by oxidizing substances and of another kind of bacteria which consume hydrogen. Various kinds of symbiotic systems including those of ...

1992-07-10

46

Weeds of California and Other Western States  

Science.gov (United States)

... Appendix (including Asclepias fruticosa, Chrysanthemum balsamita, Silene pseudatocion, Ipomea quamoclit, Ephedra distachya, Cinnamonum camphora, Papaver capreolata, Passiflora ... ...

47

NASA Information Technology Requirement  

Science.gov (United States)

Appendix C Information Technology (IT) Waiver Process. Distribution. NODlS. Center Chief Information Officers. 1. Page 3. Change History ...

48

Appendix F. List of citations accepted and rejected by ...  

Science.gov (United States)

... F4 ... Sublethal Toxicity of Nine Pesticides on Olfactory Learning Performances of ... Daphnia and Preparation of Dosage- Mortality Curves for Pesticides ...

2008-02-21

49

Appendix A 1994 Fish and Wildlife Service and National Marine Fisheries Service  

Science.gov (United States)

... is not a "significant portion of its range." Asarco, Inc. v. EPA, 616 F.2d 1153, 1159 ( ... ...

50

Annual report to Congress 1997  

Energy Technology Data Exchange (ETDEWEB)

Section 205 of the Department of Energy Organization Act of 1977 established the Energy Information Administration (EIA). One of the mandates in this legislation is that EIA prepare for Congress an annual report summarizing both activities and information collected and published. EIA`s major 1997 accomplishments are profiled in the body of this edition of the Annual Report to Congress. Appendix A contains abstracts of significant reports issued by EIA in 1997, and a chart of all titles and a list of all feature articles published during the year. Appendix B contains graphs of selected performance measures. Appendix C lists contact information for EIA subject matter specialists. Appendix D lists the major laws which form the basis of EIA`s legislative mandate.

1998-07-01

51

Role of periodontal pathogenic bacteria in RANKL-mediated bone destruction in periodontal disease  

UK PubMed Central (United Kingdom)

Accumulated lines of evidence suggest that hyperimmune responses to periodontal bacteria result in the destruction of periodontal connective tissue and alveolar bone. The etiological roles of periodontal...Full Text Available

52

Numerical Taxonomy of Some Bacteria Isolated from Antarctic and Tropical Seawaters1  

UK PubMed Central (United Kingdom)

Pfister, Robert M. (Lamont Geological Observatory, Palisades, N.Y.), and Paul R. Burkholder. Numerical taxonomy of some bacteria isolated from Antarctic and tropical seawaters. J....Full Text Available

1965-10-01

53

Nitrogen Cycling and Community Structure of Proteobacterial ?-Subgroup Ammonia-Oxidizing Bacteria within Polluted Marine Fish Farm Sediments  

UK PubMed Central (United Kingdom)

A multidisciplinary approach was used to study the effects of pollution from a marine fish farm on nitrification rates and on the community structure of ammonia-oxidizing bacteria in the underlying...Full Text Available

1999-01-01

54

Modification of Spatial Distribution of 2,4-Dichlorophenoxyacetic Acid Degrader Microhabitats during Growth in Soil Columns  

UK PubMed Central (United Kingdom)

Bacterial processes in soil, including biodegradation, require contact between bacteria and substrates. Knowledge of the three-dimensional spatial distribution of bacteria at the microscale is necessary...Full Text Available

2004-05-01

55

Lizards in the ecology of salmonellosis in Panama.  

UK PubMed Central (United Kingdom)

Enteropathogenic bacteria was isolated from 131 of 447 (29.4%) neotropical Panamanian lizards belonging to 34 species of seven families. Overall, 147 strains of bacteria were isolated comprising 26...Full Text Available

1981-05-01

56

Isolation and characterization of quinoline-degrading bacteria from subsurface sediments.  

UK PubMed Central (United Kingdom)

Two gram-negative, motile bacteria isolated from deep subsurface sediments mineralized the nitrogen-containing polyaromatic hydrocarbon quinoline under aerobic conditions and transformed quinoline to...Full Text Available

1989-04-01

57

Insights into the Genome of Large Sulfur Bacteria Revealed by Analysis of Single Filaments  

UK PubMed Central (United Kingdom)

Marine sediments are frequently covered by mats of the filamentous Beggiatoa and other large nitrate-storing bacteria that oxidize hydrogen sulfide using either oxygen or nitrate, which...Full Text Available

2007-09-01

58

Influence of Species Specificity and Other Factors on Bacteria Associated with the Coral Stylophora pistillata in Taiwan ?  

UK PubMed Central (United Kingdom)

Species of bacteria associated with Stylophora pistillata were determined by analyses of 16S ribosomal genes. Coral samples were taken from two distinct sites at Kenting, in the far...Full Text Available

2009-12-01

59

Identification of immune genes and proteins involved in the response of bovine mammary tissue to Staphylococcus aureus infection  

UK PubMed Central (United Kingdom)

BackgroundMastitis in dairy cattle results from infection of mammary tissue by a range of micro-organisms but principally coliform bacteria and Gram positive bacteria such as Staphylococcus...Full Text Available

60

Growth, cell division and sporulation in mycobacteria  

UK PubMed Central (United Kingdom)

Bacteria have the ability to adapt to different growth conditions and to survive in various environments. They have also the capacity to enter into dormant states and some bacteria form spores when...Full Text Available

2010-08-01

61

Functional Exoenzymes as Indicators of Metabolically Active Bacteria in 124,000-Year-Old Sapropel Layers of the Eastern Mediterranean Sea  

UK PubMed Central (United Kingdom)

Hydrolytic exoenzymes as indicators of metabolically active bacteria were investigated in four consecutive sapropel layers collected from bathyal sediments of the eastern Mediterranean Sea. For comparison,...Full Text Available

2000-06-01

62

Evaluation of broth disk elution methods for susceptibility testing of anaerobic bacteria with the newer beta-lactam antibiotics.  

UK PubMed Central (United Kingdom)

Broth disk elution procedures represent one of the most practical means for clinical laboratories to perform routine antibiotic susceptibility tests on anaerobic bacteria. The accuracy of five disk...Full Text Available

1986-03-01

63

Enrichment of Thermophilic Propionate-Oxidizing Bacteria in Syntrophy with Methanobacterium thermoautotrophicum or Methanobacterium thermoformicicum  

UK PubMed Central (United Kingdom)

Thermophilic propionate-oxidizing, proton-reducing bacteria were enriched from the granular methanogenic sludge of a bench-scale upflow anaerobic sludge bed reactor operated at 55°C with a mixture...Full Text Available

1992-01-01

64

Detection of Unculturable Bacteria in Periodontal Health and Disease by PCR  

UK PubMed Central (United Kingdom)

Recently developed molecular methods have made it possible to characterize mixed microflora in their entirety, including the substantial numbers of bacteria which do not grow on artificial culture media....Full Text Available

1999-05-01

65

Comparison of oral and vaginal metronidazole for treatment of bacterial vaginosis in pregnancy: impact on fastidious bacteria  

UK PubMed Central (United Kingdom)

BackgroundBacterial vaginosis (BV) is a common condition that is associated with preterm birth and acquisition of complex communities of vaginal bacteria that include several fastidious...Full Text Available

66

Chemical structure and inhalation toxicity of lipopolysaccharides from bacteria on cotton.  

UK PubMed Central (United Kingdom)

Lipopolysaccharides from different bacteria isolated from cotton were purified and chemically analyzed. Their pulmonary toxicity to animals was tested in inhalation tests. Lipopolysaccharides from Agrobacterium...Full Text Available

1980-09-01

67

Antibiotic resistance of gram-negative enteric bacteria from pigs in three herds with different histories of antibiotic exposure.  

UK PubMed Central (United Kingdom)

The antibiotic resistance patterns of gram-negative fecal bacteria from pigs in three herds with different histories of antibiotic exposure were examined. In general, smaller proportions of antibiotic-resistant...Full Text Available

1989-09-01

68

AGEs Secreted by Bacteria Are Involved in the Inflammatory Response  

UK PubMed Central (United Kingdom)

Advanced Glycated End Products (AGEs) are formed by non-enzymatic protein glycation and are implicated in several physiological aspects including cell aging and diseases. Recent data indicate that bacteria...Full Text Available

71

In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sludges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I  

UK PubMed Central (United Kingdom)

We previously showed that very thin filamentous bacteria affiliated with the division green non-sulfur bacteria were abundant in the outermost layer of thermophilic methanogenic sludge granules fed...Full Text Available

2001-12-01

72

Hard spectator interactions in B {yields} {pi}{pi} at order {alpha}{sup 2}{sub s}  

Energy Technology Data Exchange (ETDEWEB)

In the present thesis I discuss the hard spectator interaction amplitude in B {yields} {pi}{pi} at NLO i.e. at O({alpha}{sup 2}{sub s}). This special part of the amplitude, whose LO starts at O({alpha}{sub s}), is defined in the framework of QCD factorization. QCD factorization allows to separate the short- and the long-distance physics in leading power in an expansion in {lambda}{sub QCD}/m{sub b}, where the short-distance physics can be calculated in a perturbative expansion in {alpha}{sub s}. Compared to other parts of the amplitude hard spectator interactions are formally enhanced by the hard collinear scale {radical}({lambda}{sub QCD}m{sub b}), which occurs next to the mb-scale and leads to an enhancement of {alpha}{sub s}. From a technical point of view the main challenges of this calculation are due to the fact that we have to deal with Feynman integrals that come with up to five external legs and with three independent ratios of scales. These Feynman integrals have to be ...

2007-05-31

73

Air quality monitoring Lloydminster area : 2002, 2003 and 2004 seasonal monitoring : final report  

Energy Technology Data Exchange (ETDEWEB)

As part of its air quality monitoring activities, Alberta Environment conducts unannounced mobile air quality surveys each year using the Department's Mobile Air Monitoring Laboratory (MAML). This laboratory provides a snapshot of the air quality at the location monitored for a particular time and space. The MAML measures several pollutants in the air including ammonia, carbon monoxide, hydrocarbons, oxides of nitrogen, ozone, particulate matter, reduced sulphur compounds and sulphur dioxide. Alberta Environment conducted a series of ambient air quality monitoring surveys in the Lloydminster Area in 2002, 2003 and 2004. This report presents the results of the five monitored locations over eight seasons of monitoring, which were selected in consultation with stakeholders and staff from Alberta Environment's Central Region office. Canister samples were also collected during these surveys for later laboratory analyses of volatile organic compounds. Results from other ...

2005-11-01

74

Research of the ecosystem in coastal Baltic waters. Trace analysis under prior consideration of selenium and molybdenum. Final report; Oekosystemforschung in Litoralgewaessern der Ostsee. Spurenanalytik unter besonderer Beruecksichtigung von Selen und Molybdaen. Schlussbericht  

Energy Technology Data Exchange (ETDEWEB)

To study the biogeochemical behaviour, mobility and transport of trace metals in seawater (Se, Mo, Cd, Pb, Hg, Cu, Co, Fe, Ni, Zn), in suspended particulate matter (Mo, Cd, Pb, Cu, Co, Fe, Ni, Zn, Al, Mn) and in sediments of the inner and outer coastal regions, samples were collected on 3 separate cruises during a one year period (Mai 1993 - April 1994) at 20 stations in the coastal area of Mecklenburg-Vorpommern. Standard hydrographic parameters, nutrients and dissolved oxygen were also measured. Priority was taken to establish the methods for the determination of molybdenum and selenium. To study the behaviour of these two elements in detail, longterm measurements were carried out weekly between January 1993 and June 1994 at the `Messsteg/Warnemuende`. Because of parallel measurements correlations to biological, chemical and hydrographic parameters have been tested. The total database is summarized in the Appendix. The results are presented in 30 figures and 13 ...

1995-04-01

75

1981/82 nonresidential-building standards development project: economic assumptions for SOLFIN 2 analysis  

Energy Technology Data Exchange (ETDEWEB)

Documentation and use of various economic analysis tools, especially the SOLFIN 2, KRUNCH, LSAP, and SIPP computer programs, are provided. The report deals with the use of these tools to perform the social economic analysis which underlies building standards. A brief description of life-cycle cost methodology is presented. The documentation of SOLFIN 2 is included. Description of first cost calculations and life-cycle cost sensitivity analysis are given. A detailed account of the general description of how electricity costs are calculated is given in an appendix. Program listings for KRUNCH, SIPP, and LSAP are given in Appendix B. Fuel costs for low, medium, and high conservation scenarios are presented in Appendix C. (MCW)

1982-03-01

76

User's manual for the BNW-I optimization code for dry-cooled power plants. [MFCIRI Code  

Science.gov (United States)

This appendix provides a listing of the BNW-I optimization code, MFCIRI, for a metal finned tube dry-cooled heat rejection system for power plants. (LCL)

1977-01-01

77

Toward a more rigorous application of margins and uncertainties within the nuclear weapons life cycle : a Sandia perspective.  

Energy Technology Data Exchange (ETDEWEB)

This paper presents the conceptual framework that is being used to define quantification of margins and uncertainties (QMU) for application in the nuclear weapons (NW) work conducted at Sandia National Laboratories. The conceptual framework addresses the margins and uncertainties throughout the NW life cycle and includes the definition of terms related to QMU and to figures of merit. Potential applications of QMU consist of analyses based on physical data and on modeling and simulation. Appendix A provides general guidelines for addressing cases in which significant and relevant physical data are available for QMU analysis. Appendix B gives the specific guidance that was used to conduct QMU analyses in cycle 12 of the annual assessment process. Appendix C offers general guidelines for addressing cases in which appropriate models are available for use in QMU analysis. Appendix D contains an example that ...

2007-12-01

78

The Coming Nuclear Renaissance for Next Generation Safeguards Specialists--Maximizing Potential and Minimizing the Risks  

Energy Technology Data Exchange (ETDEWEB)

This document is intended to provide an overview of the workshop entitled 'The Coming Nuclear Renaissance for the Next Generation Safeguards Experts-Maximizing Benefits While Minimizing Proliferation Risks', conducted at Oak Ridge National Laboratory (ORNL) in partnership with the Y-12 National Security Complex (Y-12) and the Savannah River National Laboratory (SRNL). This document presents workshop objectives; lists the numerous participant universities and individuals, the nuclear nonproliferation lecture topics covered, and the facilities tours taken as part of the workshop; and discusses the university partnership sessions and proposed areas for collaboration between the universities and ORNL for 2009. Appendix A contains the agenda for the workshop; Appendix B lists the workshop attendees and presenters with contact information; Appendix C contains graphics of the evaluation form results and survey ...

2009-01-01

79

SEER Treatment Field Names, Edition Numbers, NAACCR Data Item Name,  

Science.gov (United States)

In addition, for breast cancer, a fifth field, Reconstruction--First Course is recorded. V.02.E Reconstruction--First Course (breast only) 1 digit These codes for these fields are included as Appendix C of this manual.

80

Office of Industrial Technologies: Summary of program results  

Energy Technology Data Exchange (ETDEWEB)

Working in partnership with industry, the US Department of Energy`s (DOE`s) Office of Industrial Technologies (OIT) is helping reduce industrial energy use, emissions, and waste while boosting productivity. Operating within the Office of Energy Efficiency and Renewable Energy (EE), OIT conducts research, development, demonstration, and technology transfer efforts that are producing substantial, measurable benefits to industry. This document summarizes some of the impacts of OIT`s programs through 1997. OIT tracks energy savings as well as other benefits associated with the successfully commercialized technologies resulting from OIT-supported research partnerships. Specifically, a chart shows current and cumulative energy savings as well as cumulative reductions of various air pollutants including particulates, volatile organic compounds (VOCs), nitrogen oxides (NO{sub x}), sulfur oxides (SO{sub x}), and the greenhouse gas, carbon dioxide (CO{sub 2}). The bulk of the document consists ...

1999-01-01

81

Microsoft Word - AKF Annual Report 2005-06.doc  

Wastenet

AUSTRALIA-KOREA FOUNDATION ...Appendix 1: Order-in-Council establishing the Australia-Korea Foundation ...has been a successful period for the Australia-Korea Foundation in working towards strengthening and broadening the

82

FS-1037C Appendix A  

Science.gov (United States)

Telecommunication Union IVDT integrated voice data terminal IXC interexchange carrier JANAP Joint Army-Navy-Air Force Publication(s) JCS Joint Chiefs of Staff JPL Jet Propulsion...

2007-05-31

83

Estimating the Costs of Unintentional Injuries  

Science.gov (United States)

... A description of the National Safety Council's current cost estimating procedures may be found in the Technical Appendix ... the 1993 bulletin, the Council extensively revised its cost estimating procedures. New components were added, new benchmarks and ...

84

Electroweak interactions and high-energy limit  

CERN Document Server

A pedagogical introduction to the equivalence theorem for longitudinal vector bosons in electroweak theories is given and the problem of tree-level unitarity at high energies in models of electroweak interactions is briefly reviewed. To make the treatment self-containded, the basic of the Standard Model are summarized in an appendix.

1996-01-01

85

Definition and means of maintaining the criticality detectors and alarms portion of the PFP safety envelope  

Energy Technology Data Exchange (ETDEWEB)

The purpose of this document is to provide the definition and means of maintaining the Safety Envelope (SE) related to the Criticality Alarm System (CAS). This document provides amplification of the Limiting Condition for Operation (LCO) described in the Plutonium Finishing Plant (PFP) Operational Safety Requirements (OSR), WHC-SD-CP-OSR-010, Rev. 0, 1994, Section 3.1.2, Criticality Detectors and Alarms. This document, with its appendices, provides the following: (1) System functional requirements for determining system operability (Section 3); (2) A list of annotated system block diagrams which indicate the safety envelope boundaries (Appendix C); (3) A list of the Safety Class 1 and 2 Safety Envelope (SC-1/2 SE) equipment for input into the Master Component Index (Appendix B); (4) Functional requirements for individual SC-1/2 SE components, including appropriate setpoints and process parameters (Section 6 and Appendix A); ...

1997-05-13

86

B-1 Primary Safety  

Science.gov (United States)

... Page 2. Boston Scientific CRM 11/17/09 MADIT-CRT Clinical Report APPENDIX B 2 CONFIDENTIAL ... B-2 Primary Effectiveness B-2.1 Validation of Assumptions ...

87

Appendix E - Summary of Ecotoxicity Data for Diquat ...  

Science.gov (United States)

... 4.65 >4.65 Jimson weed (Datura stramonium) 4.65 >4.65 Morning glory (Ipomea sp) 4.65 >4.65 Soybean (Glycine max) 4.65 >4.65 ...

2010-12-29

88

APPENDIX, GLOSSARY, AND BIBLIOGRAPHY - NASA Headquarters  

Science.gov (United States)

for sensitive personnel data. Based on the judgment of Task Force ...... Gunn, R ., Memorandum on Sub-Atomic Power Sources for Submarine ... Hunley, J., ed., The Birth of NASA: The Diary of T. Keith Glennan, SP-4105, ...

89

45 CFR Appendix to Part 1308 - Head Start Program Performance Standards on Services to Children With Disabilities  

Science.gov (United States)

...8Eligibility Criteria: Emotional/Behavioral Disorders Guidance for Paragraph...at-risk for emotional/behavioral disorders and their parents to help...Evaluation Team for Emotional/behavioral Disorders: Psychologist,...

2010-10-01

90

40 CFR 52.1370 - Identification of plan.  

Science.gov (United States)

...analysis for all of the stacks except the ASARCO stacks. (i) Incorporation by reference...1985 (except for materials pertaining to ASARCO), and January 28, 1986 (except for meterials pertaining to ASARCO and Appendix A). (19) On...

2010-07-01

91

Visualization of right atrial appendix by Thallium-201 myocardial scintigraphy: concise communication  

Energy Technology Data Exchange (ETDEWEB)

The atrial myocardium has been barely visible with thallium-201 myocardial scintigraphy. This is probably related to the difference in size, distance from anterior chest wall, and small coronary blood flow of the atrium, compared with the ventricle. We have encountered eight cases of visualization of the right atrial appendix (RAA). The RAA was identified from multiple projections in all cases and sometimes confirmed by radionuclide angiocardiography. Evidence derived from ECG, chest radiographs, and cardiac catheterization, indicated that the most important factor in the visualization might be the displacement of the RAA to a more anterior position.

1980-10-01

92

Uncoupler-resistant mutants of bacteria.  

UK PubMed Central (United Kingdom)

The chemiosmotic model of energy transduction offers a satisfying and widely confirmed understanding of the action of uncouplers on such processes as oxidative phosphorylation; the uncoupler, by facilitating...Full Text Available

1990-03-01

93

Thermokinetic investigation of effects of carbon source on petroleum bacterial growth  

Energy Technology Data Exchange (ETDEWEB)

The growth power-time curves of a strain of petroleum bacteria, B-2, in various kinds of cultures containing different kinds of carbon sources, glucose, n-tetradecane, n-hexadecane and n-octadecane, and different kinds of microemulsions have been determined by using a 2277 Thermal Activity Monitor. The curves showed a single peak for cultures containing a single carbon source, glucose, and two peaks for cultures containing two kinds of carbon sources, glucose and one of the n-alkanes. The first peak indicated that bacteria grew by consuming glucose and the second peak indicated that bacteria grew by consuming n-alkane. The curves were complex when the bacterium grows in a microemulsion culture. According to a kinetic equation of bacterial growth under limited conditions, the rate constants of bacterial growth were obtained. The results showed that the microemulsion culture was more appropriate to ...

2002-02-07

94

Sirtuins, Bioageing, and Cancer  

UK PubMed Central (United Kingdom)

The Sirtuins are a family of orthologues of yeast Sir2 found in a wide range of organisms from bacteria to man. They display a high degree of conservation between species, in both sequence and function,...Full Text Available

95

Probiotic Bacteria as Biological Control Agents in Aquaculture  

UK PubMed Central (United Kingdom)

There is an urgent need in aquaculture to develop microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and trade and since the development...Full Text Available

2000-12-01

96

Factors Influencing the Effectiveness of Swimming Pool Bactericides  

UK PubMed Central (United Kingdom)

Techniques for culturing, harvesting, and testing bacteria to evaluate bactericidal chemicals for swimming pools are described. Concentrations of 25, 50, and 100 mg of the chlorine stabilizer cyanuric...Full Text Available

1967-05-01

97

FLUOROMETRIC MEASUREMENT OF OXIDATIVE BURST IN LOBSTER HEMOCYTES AND INHIBITING EFFECT OF PATHOGENIC BACTERIA AND ...  

Science.gov (United States)

... Hunter. 2003. The resistance to ammonia stress of Penaeus monodon Fabricius juveniles fed diets supplemented with astaxanthin. J. Exp. ... ...

98

Eukaryotic Diversity in an Anaerobic Aquifer Polluted with Landfill Leachate?  

UK PubMed Central (United Kingdom)

Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and...Full Text Available

2008-07-01

99

Detection and Identification of Bacteria by Gas Chromatography1  

UK PubMed Central (United Kingdom)

Ether extracts of cultures of 29 strains representing 6 species of Bacillus, and of individual strains of Escherichia coli, Aerobacter aerogenes, and Pseudomonas...Full Text Available

1966-07-01

100

Degradation of malathion by salt-marsh microorganisms.  

UK PubMed Central (United Kingdom)

Numerous bacteria from a salt-marsh environment are capable of degrading malathion, an organophosphate insecticide, when supplied with additional nutrients as energy and carbon sources. Seven isolates...Full Text Available

1977-02-01

101

Biosorption of heavy metal ions on Rhodobacter sphaeroides and Alcaligenes eutrophus H16  

Energy Technology Data Exchange (ETDEWEB)

A fundamental study of the application of bacteria to the recovery of toxic heavy metals from aqueous environments was carried out. The biosorption characteristics of cadmium and lead ions were determined with purple nonsulfur bacteria, Rhodobacter sphaeroides and hydrogen bacteria, Alcaligenes eutrophus H16 that were inactivated by steam sterilization. A simplified version of the metal binding model proposed by Plette et al. was used for the description of meal binding data. The results showed that the biosorption of bivalent metal ions to whole cell bodies of the bacteria was due to monodentate binding to two different types of acidic sites: carboxilic and phosphatic-type sites. The number of metal binding sites of A. eutrophus was 2.4-fold larger than that of R. sphaeroides.

1998-01-15

102

Investigation of Nuclide Importance to Functional Requirements Related to Transport and Long-Term Storage of LWR Spent Fuel  

Energy Technology Data Exchange (ETDEWEB)

The radionuclide characteristics of light-water-reactor (LWR) spent fuel play key roles in the design and licensing activities for radioactive waste transportation systems, interim storage facilities, and the final repository site. Several areas of analysis require detailed information concerning the time-dependent behavior of radioactive nuclides including (1) neutron/gamma-ray sources for shielding studies, (2) fissile/absorber concentrations for criticality safety determinations, (3) residual decay heat predictions for thermal considerations, and (4) curie and/or radiological toxicity levels for materials assumed to be released into the ground/environment after long periods of time. The crucial nature of the radionuclide predictions over both short and long periods of time has resulted in an increased emphasis on thorough validation for radionuclide generation/depletion codes. Current radionuclide generation/depletion codes have the capability to follow the ...

1995-01-01

103

The microbiology of forest soils: a literature review  

Energy Technology Data Exchange (ETDEWEB)

This report discusses the activities of two major groups of forest soil microorganisms, the bacteria and the fungi. Special attention is paid to their participation in the decay of major forest litter substrates, including leaves, branches and roots. The influence of bacteria and fungi in symbiotic associations with woody plant roots upon the cycles of carbon and nitrogen is described. The impacts of certain forest mamagement alternatives are assessed in terms of the creation of elimination of suitable environments for the activity of soil microorganisms. A bibliography is included. 507 refs., 1 tab.

1982-01-01

104

Extended Spectrum ?-Lactamases among Gram-negative bacteria of nosocomial origin from an Intensive Care Unit of a tertiary health facility in Tanzania  

UK PubMed Central (United Kingdom)

BackgroundResistance to third generation cephalosporins due to acquisition and expression of extended spectrum β-lactamase (ESBL) enzymes among Gram-negative bacteria is...Full Text Available

105

Elucidating novel pathways and regulation of nitrogen assimilation in alpha proteobacteria exemplified by the soil organism Paracoccus denitrificans  

Environmental Research Database

DescriptionThe importance of inorganic nitrate for the nutrition and growth of marine and freshwater autotrophic phytoplankton has long been recognised, in particularly because of the 'blooms' formed in nitrate and phosphate polluted water and their possible roles as carbon dioxide sinks. By contrast, the utilisation of nitrate by heterotrophic bacteria has historically received less attention. Up until now, the primary role of heterotrophic bacteria has classically been considered to be the decomposition [continued...

2010-01-31

106

Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens.  

UK PubMed Central (United Kingdom)

Fifteen strains of CDC group 1 coryneform and biochemically similar bacteria were isolated from clinical specimens. Of the 15 strains isolated, 11 were derived from abscesses and purulent lesions, mostly...Full Text Available

1993-11-01

107

Development and application of microbial selective plugging processes  

Energy Technology Data Exchange (ETDEWEB)

Phillips Petroleum Company recently completed a microbial selective plugging (MSP) pilot at the North Burbank Unit (NBU), Shidler, Oklahoma. Nutrients were selected for the pilot that could stimulate indigenous microflora in the reservoir brine to grow and produce exopolymer. It was found that soluble corn starch polymers (e.g., maltodextrins) stimulated the indigenous bacteria to produce exopolymer, whereas simple sugars (e.g., glucose and sucrose), as well as complex media (e.g., molasses and Nutrient Broth), did not. Injection of maltodextrin into rock cores in the presence of indigenous NBU bacteria resulted in stable permeability reductions (> 90%) across the entire length, while injection of glucose resulted only in face plugging. In addition, it was found that organic phosphate esters (OPE) served as a preferable source of phosphorus for the indigenous bacteria, since orthophosphates and condensed phosphates ...

1995-12-31

108

Cost-effectiveness analysis of proposed effluent limitations guidelines and standards for the transportation equipment cleaning category  

Science.gov (United States)

This cost-effectiveness analysis presents an evaluation of the technical efficiency of pollutant control options for the proposed Effluent Limitations Guidelines and Standards for the Transportation Equipment Cleaning Industry based on Best Available Technology Economically Achievable (BAT) and Pretreatment Standards for Existing Sources (PSES). Section 2 discusses EPA`s cost-effectiveness methodology and identifies the pollutants included in the analysis. This section also presents EPA`s toxic weighting factors for each pollutant and considers the removal efficiency of each pollution control option. Section 3 describes the options evaluated for each subcategory. Section 4 presents the results of the cost-effectiveness analysis. In Section 5, cost-effectiveness values for proposed TEC industry options are compared to cost-effectiveness values for other promulgated rules. Section 6 discusses the two-part cost-reasonableness test for BCT options. Appendix A presents ...

1998-05-01

109

Thermal neutron capture gamma-rays  

Energy Technology Data Exchange (ETDEWEB)

The energy and intensity of gamma rays as seen in thermal neutron capture are presented. Only those (n,..cap alpha..), E = thermal, reactions for which the residual nucleus mass number is greater than or equal to 45 are included. These correspond to evaluations published in Nuclear Data Sheets. The publication source data are contained in the Evaluated Nuclear Structure Data File (ENSDF). The data presented here do not involve any additional evaluation. Appendix I lists all the residual nuclides for which the data are included here. Appendix II gives a cumulated index to A-chain evaluations including the year of publication. The capture gamma ray data are given in two tables - the Table 1 is the list of all gamma rays seen in (n,..gamma..) reaction given in the order of increasing energy; the Table II lists the gamma rays according to the nuclide.

1983-01-01

110

Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds.  

Science.gov (United States)

Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gram-negative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 10(5) c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species- and strain-specific. Streptococcus pyogenes was the ...

2010-09-09

111

Use of radiosensitivity to identify irradiated fresh poultry products  

International Nuclear Information System (INIS)

Microbiological comparison between irradiated and non-irradiated foodstuff has been studied for a long time as a way to detect whether a foodstuff has been irradiated or not. Generally, the proposed methods are based on the fact that ionization select species of bacteria which are recognized to be radioresistant. So reduction or elimination of known radiation sensitive microbes from the normal endogenous microflora could give an indication that the foodstuff has been irradiated, predominance of known radioresistant bacteria should be another indication. In the present work, we try to develop a test based on the radiosensitivity of the bacteria independently of their place. These first experiments show that the determination of radiosensitivity of strains isolated from a product or even of global radioresistance of mesophilic microflora could indicate if this product has been previously submitted to ionizing radiations. (4 ...

1990-02-14

112

Strength and stability of microbial plugs in porous media  

Energy Technology Data Exchange (ETDEWEB)

Mobility reduction induced by the growth and metabolism of bacteria in high-permeability layers of heterogeneous reservoirs is an economically attractive technique to improve sweep efficiency. This paper describes an experimental study conducted in sandpacks using an injected bacterium to investigate the strength and stability of microbial plugs in porous media. Successful convective transport of bacteria is important for achieving sufficient initial bacteria distribution. The chemotactic and diffusive fluxes are probably not significant even under static conditions. Mobility reduction depends upon the initial cell concentrations and increase in cell mass. For single or multiple static or dynamic growth techniques, permeability reduction was approximately 70% of the original permeability. The stability of these microbial plugs to increases in pressure gradient and changes in cell physiology in a nutrient-depleted ...

1995-12-31

113

PREPARATION AND PROPERTIES OF ANTIBACTERIAL ALGINATE FILMS INCORPORATING EXTRUDED WHITE GINSENG EXTRACT  

British Library Electronic Table of Contents (United Kingdom)

Abstract Preparation of antibacterial alginate films incorporating extruded white ginseng (EWG) extracts was attempted. The antibacterial effect of EWG extract on six selected food pathogenic bacteria was compared with the effect of red ginseng (RG) and white ginseng (WG) extracts. The EWG was processed in a twin-screw extruder with feed moisture of 20% and barrel temperature of 115 and 130C. The data obtained by agar diffusion assay demonstrated that the film containing 1-g/mL of EWG at barrel temperatures of 115 (EWG-115) and 130C (EWG-130) exhibited stronger antibacterial activity against the four strains of bacteria than the other extracts RG and WG. All films sampled showed reduction in bacteria cell counts (log cycle) compared with the control. After 24-h of incubation, Pseudomonas a...

2011-01-01

114

Optimal conditions for stability of photoemission and freeze drying of two luminescent bacteria for use in a biosensor  

British Library Electronic Table of Contents (United Kingdom)

Abstract Bioluminescent bacteria have been used for many years for biotoxicological analysis. One of the main concerns with this microorganism is the low experimental repeatability when subjected to external factors. The aim of the present study was to obtain accurate, sensitive, and repeatable measurements with stable signals (during the detection and over days) for application in a water-analysis device for the detection of pollutants. Growth conditions were tested and optimized. An optimal freeze-drying procedure for the constitutive bioluminescent bacteria Vibrio fischeri and Photobacterium phosphoreum was developed. The luminescence stability after rehydration was also investigated. Freeze drying was found to be a critical process in survival and signal stability of luminescent bacter...

2011-01-01

115

Leaching of zinc sulfide by Thiobacillus ferrooxidans: Bacterial oxidation of the sulfur product layer increases the rate of zinc sulfide dissolution at high concentrations of ferrous ions  

Energy Technology Data Exchange (ETDEWEB)

This paper reports the results of leaching experiments conducted with and without Thiobacillus ferroxidans at the same conditions in solution. The extent of leaching of ZnS with Bacteria is significantly higher than that without bacteria at high concentrations of ferrous ions. A porous layer of elemental sulfur is present on the surfaces of the chemically leached particles, which no sulfur is present on the surfaces of the bacterially leached particles. The analysis of the data using the shrinking-core model shows that the chemical leaching of ZnS is limited by the diffusion of ferrous ions through the sulfur product layer at high concentrations of ferrous ions. The analysis of the data shows that diffusion through the product layer does not limit the rate of dissolution when bacteria are present. This suggests that the action of T.ferroxidans in oxidizing the sulfur formed on the particle surface is to remove the barrier ...

1999-12-01

116

Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria  

British Library Electronic Table of Contents (United Kingdom)

Studies were carried out on the decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Among the 27 strains of halophilic and halotolerant bacteria isolated from effluents of textile industries, three showed remarkable ability in decolorizing the widely utilized azo dyes. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that these strains belonged to the genus Halomonas. The three strains were able to decolorize azo dyes in a wide range of NaCl concentration (up to 20%w/v), temperature (25-40degreeC), and pH (5-11) after 4 days of incubation in static culture. They could decolorize the mixture of dyes as well as pure dyes. These strains also readily grew in and decolorized the high concentrations of dy...

2007-01-01

117

ExternE - valuation of hydro power in Klippen. Final report; ExternE - vaerdering av vattenkraft i Klippen. Slutrapport  

Energy Technology Data Exchange (ETDEWEB)

ExternE is a pan{sub E}uropean project aimed at identifying and quantifying external effects from different energy production technologies around Europe. The Swedish implementation carried out case studies on a biomass plant, a coal plant and a hydro power plant. The conventional approach within the ExternE project is to estimate impacts for each priority pathway associated with the fuel cycle, and then economic methods to obtain estimates for the economic damage of each particular impact pathway. However, in the case of hydro power, it is generally not possible to estimate the most important impacts quantitatively. Therefore, a contingent valuation (CV) study was initiated. In a typical CV-study, a selection of individuals are asked to state their willingness to pay for a certain environmental improvement or to avoid an environmental damage. Welfare economic theory suggests that the well-being effects on people from a change from one situation to another can be measured as their ...

1998-08-01

118

Vancomycin-resistant Enterococcus domination of intestinal microbiota is enabled by antibiotic treatment in mice and precedes bloodstream invasion in humans  

UK PubMed Central (United Kingdom)

Bloodstream infection by highly antibiotic-resistant bacteria, such as vancomycin-resistant Enterococcus (VRE), is a growing clinical problem that increasingly defies medical intervention....Full Text Available

2010-12-01

119

USGS Water-Quality Information: Fecal Indicator Bacteria  

Science.gov (United States)

USGS - science for a changing world USGS Home Contact USGS Search USGS USGS Water-Quality Information * Home * Data * Methods * Labs * Publications * Topics * Programs * Contact Us...

2011-08-27

120

Type IVB Secretion Systems of Legionella and Other Gram-Negative Bacteria  

UK PubMed Central (United Kingdom)

Type IV secretion systems (T4SSs) play a central role in the pathogenicity of many important pathogens, including Agrobacterium tumefaciens, Helicobacter pylori, and...Full Text Available

121

Toxic Effects of Linear Alkylbenzene Sulfonate on Metabolic Activity, Growth Rate, and Microcolony Formation of Nitrosomonas and Nitrosospira Strains  

UK PubMed Central (United Kingdom)

Strong inhibitory effects of the anionic surfactant linear alkylbenzene sulfonate (LAS) on four strains of autotrophic ammonia-oxidizing bacteria (AOB) are reported. Two Nitrosospira...Full Text Available

2001-06-01

122

The prosthetic group of methanol dehydrogenase. Purification and some of its properties.  

UK PubMed Central (United Kingdom)

Methanol dehydrogenases isolated from bacteria belonging to different classes of methylotrophs contain the same prosthetic group. A procedure for its purification from whole cells is given. The reduced...Full Text Available

1980-04-01

123

The efflux of a fluorescent probe is catalyzed by an ATP-driven extrusion system in Lactococcus lactis.  

UK PubMed Central (United Kingdom)

Many bacteria, both gram positive and gram negative, extrude in an energy-dependent manner the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5[and -6]-carboxyfluorescein (BCECF) (D. Molenaar,...Full Text Available

1992-05-01

124

The control of sulphate reduction in bacteria  

UK PubMed Central (United Kingdom)

1. An enzyme from Escherichia coli 9723 that reduces adenosine 3′-phosphate 5′-sulphatophosphate to inorganic sulphite is described. Extracts of E. coli...Full Text Available

1965-07-01

125

The bacterial signal indole increases epithelial-cell tight-junction resistance and attenuates indicators of inflammation  

UK PubMed Central (United Kingdom)

Interkingdom signaling is established in the gastrointestinal tract in that human hormones trigger responses in bacteria; here, we show that the corollary is true, that a specific bacterial signal,...Full Text Available

2010-01-05

126

The RNA Chaperone Hfq Is Important for Growth and Stress Tolerance in Francisella novicida  

UK PubMed Central (United Kingdom)

The RNA-binding protein Hfq is recognized as an important regulatory factor in a variety of cellular processes, including stress resistance and pathogenesis. Hfq has been shown in several bacteria to...Full Text Available

127

The Pseudomonas aeruginosa PA01 Gene Collection  

UK PubMed Central (United Kingdom)

Pseudomonas aeruginosa, a common inhabitant of soil and water, is an opportunistic pathogen of growing clinical relevance. Its genome, one of the largest among bacteria [5570 open reading...Full Text Available

2004-10-01

128

Surprising Alteration of Antibacterial Activity of 5?-Modified Neomycin against Resistant Bacteria  

UK PubMed Central (United Kingdom)

A facile synthetic protocol for the production of neomycin B derivatives with various modifications at the 5″ position has been developed. Structural activity relationship (SAR) against...Full Text Available

2008-12-11

129

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1  

UK PubMed Central (United Kingdom)

Several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. One isolate, strain D, was selected for characterization. This organism...Full Text Available

1974-09-01

130

Succession of Indigenous Pseudomonas spp. and Actinomycetes on Barley Roots Affected by the Antagonistic Strain Pseudomonas fluorescens DR54 and the Fungicide Imazalil  

UK PubMed Central (United Kingdom)

In recent years, the interest in the use of bacteria for biological control of plant-pathogenic fungi has increased. We studied the possible side effects of coating barley seeds with the antagonistic...Full Text Available

2001-03-01

131

Structural basis of ligand binding by a c-di-GMP riboswitch  

UK PubMed Central (United Kingdom)

The second messenger signaling molecule bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) regulates many processes in bacteria, including motility, pathogenesis,...Full Text Available

2009-12-01

132

Sex and space destabilize intransitive competition within and between species  

UK PubMed Central (United Kingdom)

Organisms ranging from bacteria and corals to plants and vertebrates can form intransitive competitive networks, in which coexistence can be maintained because no one species or genotype is superior...Full Text Available

2008-08-22

133

Self-Chaperoning of the Type III Secretion System needle tip proteins IpaD and BipD  

UK PubMed Central (United Kingdom)

Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid...Full Text Available

2007-02-09

134

Reduction of Selenate to Selenide by Sulfate-Respiring Bacteria: Experiments with Cell Suspensions and Estuarine Sediments  

UK PubMed Central (United Kingdom)

Washed cell suspensions of Desulfovibrio desulfuricans subsp. aestuarii were capable of reducing nanomolar levels of selenate to selenide as well as sulfate to sulfide....Full Text Available

1987-06-01

135

Pseudomonas aeruginosa selective adherence to and entry into human endothelial cells.  

UK PubMed Central (United Kingdom)

The pathogenesis of Pseudomonas aeruginosa disseminated infections depends on bacterial interaction with blood vessels. We have hypothesized that in order to traverse the endothelial barrier, bacteria...Full Text Available

1994-12-01

136

Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum.  

UK PubMed Central (United Kingdom)

We have examined the expression and structure of several genes belonging to two classes of vegetative specific genes of the simple eukaryote, Dictyostelium discoideum. In amebae grown on bacteria, deactivation...Full Text Available

1989-12-11

137

Preterm low birthweight and the role of oral bacteria  

UK PubMed Central (United Kingdom)

Preterm and low birthweight (PTLBW) continues to be a major cause of mortality and morbidity across the world. In recent years, maternal periodontal disease has been implicated as a risk factor for...Full Text Available

138

Physiological Adaptation of a Nitrate-Storing Beggiatoa sp. to Diel Cycling in a Phototrophic Hypersaline Mat?  

UK PubMed Central (United Kingdom)

The aim of this study was to investigate the supposed vertical diel migration and the accompanying physiology of Beggiatoa bacteria from hypersaline microbial mats. We combined microsensor,...Full Text Available

2007-11-01

139

PCR-based method for targeting 16S-23S rRNA intergenic spacer regions among Vibrio species  

UK PubMed Central (United Kingdom)

BackgroundThe genus Vibrio is a diverse group of Gram-negative bacteria comprised of 74 species. Furthermore, the genus has and is expected to continue expanding...Full Text Available

140

On Being the Right Size: The Impact of Population Size and Stochastic Effects on the Evolution of Drug Resistance in Hospitals and the Community  

UK PubMed Central (United Kingdom)

The evolution of drug resistant bacteria is a severe public health problem, both in hospitals and in the community. Currently, some countries aim at concentrating highly specialized services in large...Full Text Available

2011-04-01

141

Occurrence of fecal indicator bacteria in surface waters and the subsurface aquifer in Key Largo, Florida.  

UK PubMed Central (United Kingdom)

Sewage waste disposal facilities in the Florida Keys include septic tanks and individual package plants in place of municipal collection facilities in most locations. In Key Largo, both facilities discharge...Full Text Available

1995-06-01

142

Name of Presentation!  

Wastenet

Up-flow anaerobic attached-growth bioreactors filled with pre-treated coir fibres ...coir-fibre arranged in bottle-brush configuration bounded by a novel plastic binding technique ...-three anaerobic filter reactors in series -coir fibre as the bacteria growth media a sedimentation

143

Mutagenic and cytotoxic activity of doxorubicin and daunorubicin derivatives on prokaryotic and eukaryotic cells.  

UK PubMed Central (United Kingdom)

The mutagenic and cytotoxic activity of two newly synthesized doxorubicin derivatives and of one daunorubicin derivative were studied in V79 Chinese hamster cells and bacteria (Salmonella typhimurium...Full Text Available

1984-07-01

144

Isolation and characterization of a novel gamma-hexachlorocyclohexane-degrading bacterium.  

UK PubMed Central (United Kingdom)

The natural biotic capacity of soils to degrade gamma-hexachlorocyclohexane (gamma-HCH, lindane) was estimated using an enrichment technique based on the ability of soil bacteria to develop on synthetic...Full Text Available

1996-10-01

145

Induction of autolysis in nongrowing Escherichia coli.  

UK PubMed Central (United Kingdom)

Unless relaxation of the stringent response is achieved, all nongrowing bacteria rapidly develop resistance to autolysis induced by a variety of agents, including all classes of cell wall synthesis...Full Text Available

1986-09-01

146

Identification of CTX-M-Type Extended-Spectrum-?-Lactamase Genes Using Real-Time PCR and Pyrosequencing?  

UK PubMed Central (United Kingdom)

CTX-M extended-spectrum β-lactamases (ESBLs) are increasingly prevalent worldwide among Escherichia coli bacteria, mostly in community-acquired urinary tract infections. Finding...Full Text Available

2007-01-01

147

Heavy metals alter the electrokinetic properties of bacteria, yeasts, and clay minerals.  

UK PubMed Central (United Kingdom)

The electrokinetic patterns of four bacterial species (Bacillus subtilis, Bacillus megaterium, Pseudomonas aeruginosa, and Agrobacterium radiobacter), two yeasts (Saccharomyces cerevisiae and Candida...Full Text Available

1992-05-01

148

Gingival crevicular fluid levels of clindamycin compared with its minimal inhibitory concentrations for periodontal bacteria.  

UK PubMed Central (United Kingdom)

Clindamycin concentrations in gingival crevicular fluid and in blood were determined over a 7-h period and were related to the minimal inhibitory concentrations of this agent for 340 bacterial strains...Full Text Available

1981-05-01

149

Generation of Novel Bacterial Regulatory Proteins That Detect Priority Pollutant Phenols  

UK PubMed Central (United Kingdom)

The genetic systems of bacteria that have the ability to use organic pollutants as carbon and energy sources can be adapted to create bacterial biosensors for the detection of industrial pollution....Full Text Available

2000-01-01

150

Evolutionary Trajectories of Beta-Lactamase CTX-M-1 Cluster Enzymes: Predicting Antibiotic Resistance  

UK PubMed Central (United Kingdom)

Extended-spectrum beta-lactamases (ESBL) constitute a key antibiotic-resistance mechanism affecting Gram-negative bacteria, and also an excellent model for studying evolution in real time. A shift in...Full Text Available

2010-01-01

151

Epidemiological Risk Factors for Isolation of Ceftriaxone-Resistant versus -Susceptible Citrobacter freundii in Hospitalized Patients  

UK PubMed Central (United Kingdom)

Antimicrobial resistance is an emerging problem among nosocomial bacteria. Risk factors for the recovery of ceftriaxone-resistant (CRCF) or -susceptible (CSCF) Citrobacter freundii...Full Text Available

2003-09-01

152

Endometrial biopsy in Holstein-Friesian dairy cows. III. Bacteriological analysis and correlations with histological findings.  

UK PubMed Central (United Kingdom)

This study examines the results of bacterial culture from 159 endometrial biopsy samples from 97 commercial dairy cows and correlations between bacteriological and histological findings. Bacteria were...Full Text Available

1991-04-01

153

Endogenous Nitric Oxide Protects Bacteria Against a Wide Spectrum of Antibiotics  

UK PubMed Central (United Kingdom)

Bacterial nitric oxide synthases (bNOS) are present in many Gram-positive species and have been demonstrated to synthesize NO from arginine in vitro and in vivo. However, the physiological role...Full Text Available

2009-09-11

154

Effect of clindamycin on neutrophil killing of gram-negative periodontal bacteria.  

UK PubMed Central (United Kingdom)

Periodontal diseases are infections of the tissues supporting the dentition. Recognition that relatively specific microfloras are associated with distinct clinical forms of periodontal disease has prompted...Full Text Available

1988-10-01

155

Effect of Length of Time before Incorporation on Survival of Pathogenic Bacteria Present in Livestock Wastes Applied to Agricultural Soil  

UK PubMed Central (United Kingdom)

In response to reports that the contamination of food can occur during the on-farm primary phase of food production, we report data that describes a possible cost-effective intervention measure. The...Full Text Available

2004-09-01

156

Dynamics of Microbial Communities on Marine Snow Aggregates: Colonization, Growth, Detachment, and Grazing Mortality of Attached Bacteria  

UK PubMed Central (United Kingdom)

We studied the dynamics of microbial communities attached to model aggregates (4-mm-diameter agar spheres) and the component processes of colonization, detachment, growth, and grazing mortality. Agar...Full Text Available

2003-06-01

157

Distribution Frequency of Pathogenic Bacteria Isolated from Cutaneus Leishmaniasis Lesions  

UK PubMed Central (United Kingdom)

Cutaneous leishmaniasis (CL) is a parasitic disease characterized by single or multiple ulcerations. Secondary bacterial infections are one of the complications that can increase the tissue destruction...Full Text Available

2008-09-01

158

Cyclic Diguanylate Signaling Proteins Control Intracellular Growth of Legionella pneumophila  

UK PubMed Central (United Kingdom)

Proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. These processes include motility, biofilm formation, cell...Full Text Available

159

Critical superparamagnetic/single-domain grain sizes in interacting magnetite particles: implications for magnetosome crystals  

UK PubMed Central (United Kingdom)

Magnetotactic bacteria contain chains of magnetically interacting crystals (magnetosome crystals), which they use for navigation (magnetotaxis). To improve magnetotaxis efficiency, the magnetosome crystals...Full Text Available

2009-12-06

160

Coryneform bacteria in infectious diseases: clinical and laboratory aspects.  

UK PubMed Central (United Kingdom)

Coryneform isolates from clinical specimens frequently cannot be identified by either reference laboratories or research laboratories. Many of these organisms are skin flora that belong to a large number...Full Text Available

1990-07-01

161

Comparison of maintenance energy expenditures and growth yields among several rumen bacteria grown on continuous culture.  

Science.gov (United States)

Maintenance energy expenditures were mesured for five rumen bacteria, Selenomonas ruminantium, Butyrivibrio fibrisolvens, Bacteroides ruminicola, Megasphaera elsdenii, and Streptococcus bovis, by using a complex medium with glucose as the carbon source. Large differences (as high as 8.5-fold) in maintenance energy expenditures were seen among these bacteria. The suggestion is made that maintenance requirements could be a significant determinant of bacterial competition in the rumen. Theoretical maximum growth yields, calculated from double reciprocal plots of yield versus dilution rate, were compared to theoretical Y(ATP) values in order to estimate minimum molar adenosine 5'-triphosphate yields from glucose for each bacterium. Results showed that relative yield among the bacteria was growth rate dependent. At high dilution rates, both S. ruminantium and S. bovis produced lactate as their principal fermentation product. At ...

1979-03-01

162

Comparative in vitro activity of Meropenem, Imipenem and Piperacillin/tazobactam against 1071 clinical isolates using 2 different methods: a French multicentre study  

UK PubMed Central (United Kingdom)

BackgroundMeropenem is a carbapenem that has an excellent activity against many gram-positive and gram-negative aerobic, facultative, and anaerobic bacteria. The major objective...Full Text Available

163

Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea, and their metagenomes  

UK PubMed Central (United Kingdom)

BackgroundStructured noncoding RNAs perform many functions that are essential for protein synthesis, RNA processing, and gene regulation. Structured RNAs can be detected by comparative...Full Text Available

2010-01-01

164

Community Structure of Ammonia-Oxidizing Bacteria within Anoxic Marine Sediments  

UK PubMed Central (United Kingdom)

The potential for oxidation of ammonia in anoxic marine sediments exists through anaerobic oxidation by Nitrosomonas-like organisms, utilizing nitrogen dioxide, coupling of nitrification,...Full Text Available

2003-03-01

165

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.  

UK PubMed Central (United Kingdom)

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

166

Cloning and expression of portions of the 34-kilodalton-protein gene of Mycobacterium paratuberculosis: its application to serological analysis of Johne's disease.  

UK PubMed Central (United Kingdom)

Paratuberculosis (Johne's disease), an endemic mycobacteriosis of cattle that is caused by Mycobacterium paratuberculosis, is characterized by incoercible diarrhea and fecal shedding of bacteria. The...Full Text Available

1993-04-01

167

Chemical and biological monitoring of MIOR on the pilot area of Vyngapour oil field, West Sibera, Russia  

Energy Technology Data Exchange (ETDEWEB)

The pilot area of the Vyngapour oil field allotted for MIOR tests contains three injection and three producing wells. These wells were treated in summer 1993 and 1994. Before, during, and after MIOR treatments on the pilot area the chemical compounds of injected and formation waters were studied, as well as the amount and species of microorganisms entering the stratum with the injected water and indigenous bacteria presented in bottomhole zones of the wells. The results of monitoring showed that the bottomhole zone of the injection well already had biocenosis of heterotrophic, hydrocarbon-oxidizing, methanogenic, and sulfate-reducing bacteria, which were besides permanently introduced into the reservoir during the usual waterflooding. The nutritious composition activated vital functions of all bacterial species presented in the bottomhole zone of the injection well. The formation waters from producing wells showed the increase of the content of ...

1995-12-31

168

Characterization of Airborne Microbial Communities at a High-Elevation Site and Their Potential To Act as Atmospheric Ice Nuclei?  

UK PubMed Central (United Kingdom)

Bacteria and fungi are ubiquitous in the atmosphere. The diversity and abundance of airborne microbes may be strongly influenced by atmospheric conditions or even influence atmospheric conditions themselves...Full Text Available

2009-08-01

169

Changes in Tetracycline Susceptibility of Enteric Bacteria following Switching to Nonmedicated Milk Replacer for Dairy Calves?  

UK PubMed Central (United Kingdom)

A randomized intervention study was conducted to determine if discontinuing use of calf milk replacer medicated with oxytetracycline results in increased tetracycline susceptibility in Salmonella...Full Text Available

2008-06-01

170

Buoyant Densities and Dry-Matter Contents of Microorganisms: Conversion of a Measured Biovolume into Biomass  

UK PubMed Central (United Kingdom)

Several isolates of bacteria and fungi from soil, together with cells released directly from soil, were studied with respect to buoyant density and dry weight. The specific volume (cubic centimeters...Full Text Available

1983-04-01

171

Attachment Stimulates Exopolysaccharide Synthesis by a Bacterium  

UK PubMed Central (United Kingdom)

This study examined the hypothesis that solid surfaces may stimulate attached bacteria to produce exopolymers. Addition of sand to shake-flask cultures seemed to induce exopolymer synthesis by a number...Full Text Available

1993-10-01

172

Association of Nasophyaryngeal Bacterial Colonization during Upper Respiratory Tract Infection and the Development of Acute Otitis Media  

UK PubMed Central (United Kingdom)

Acute Otitis Media occurs mostly after upper respiratory tract infection; the causative bacteria are those colonized in the nasopharynx. We studied 709 URI episodes and found that children with...Full Text Available

2008-02-15

173

Antimicrobial susceptibilities of bacteria associated with periodontal disease.  

UK PubMed Central (United Kingdom)

A total of 193 bacterial strains were tested for their susceptibilities to 14 antimicrobial agents. Penicillin G was active at 2 U/ml against 98% of the oral isolates. Other antibiotics with good activity...Full Text Available

1983-03-01

174

Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments  

UK PubMed Central (United Kingdom)

The distribution and activity of communities of sulfate-reducing bacteria (SRB) and methanogenic archaea in two contrasting Antarctic sediments were investigated. Methanogenesis dominated in freshwater...Full Text Available

2003-06-01

175

Analysis of genomic differences among Clostridium botulinum type A1 strains  

UK PubMed Central (United Kingdom)

BackgroundType A1 Clostridium botulinum strains are a group of Gram-positive, spore-forming anaerobic bacteria that produce a genetically, biochemically, and biophysically...Full Text Available

176

Aggregation Substance Promotes Adherence, Phagocytosis, and Intracellular Survival of Enterococcus faecalis within Human Macrophages and Suppresses Respiratory Burst  

UK PubMed Central (United Kingdom)

The aggregation substance (AS) of Enterococcus faecalis, encoded on sex pheromone plasmids, is a surface-bound glycoprotein that mediates aggregation between bacteria thereby facilitating...Full Text Available

2000-09-01

177

Activity of Four Cephalosporin Antibiotics In Vitro Against Bovine Udder Pathogens and Pathogenic Bacteria Isolated from Newborn Calves  

UK PubMed Central (United Kingdom)

The in vitro activity of chephaloridine, cephalexin, cefatrizine (BL-S640), and cephapirin (BL-P-1322) was evaluated by the serial dilution method against pathogenic gram-positive and gram-negative...Full Text Available

1976-03-01

178

ADP-Glucose Pyrophosphorylase, a Regulatory Enzyme for Bacterial Glycogen Synthesis  

UK PubMed Central (United Kingdom)

The accumulation of α-1,4-polyglucans is an important strategy to cope with transient starvation conditions in the environment. In bacteria and plants, the synthesis of glycogen and starch occurs...Full Text Available

2003-06-01

179

A survey of the sensitivity of fresh clinical isolates to cefuroxime and other antibiotics.  

UK PubMed Central (United Kingdom)

The sensitivities to cefuroxime and cephradine of potentially pathogenic bacteria isolated in two British general hospitals comprising 900 beds have been assessed. In a three-month period 2537 strains...Full Text Available

1979-09-01

180

A small RNA promotes siderophore production through transcriptional and metabolic remodeling  

UK PubMed Central (United Kingdom)

Siderophores are essential factors for iron (Fe) acquisition in bacteria during colonization and infection of eukaryotic hosts, which restrain iron access through iron-binding protein, such as lactoferrin...Full Text Available

2010-08-24

181

A role of ygfZ in the Escherichia coli response to plumbagin challenge  

UK PubMed Central (United Kingdom)

Plumbagin is found in many herbal plants and inhibits the growth of various bacteria. Escherichia coli strains are relatively resistant to this drug. The mechanism of resistance is...Full Text Available

182

Result of the survey about radioactivity and its logotype  

International Nuclear Information System (INIS)

Those who for a long time have been performed studies with relation by affinity with radioactivity, have in mind that several basic concept to nuclear sciences are of public or general knowledge assuming that in the market there exist a wide stock of diffusion material. Nevertheless, for the attained results in this work, we have seen that there exist great disinformation about to radioactivity its peaceful uses and particularly with a logotype which indicate the presence of radioactive material. The study was carried out in the considering that a probabilistic sampling in the Mexico city will permit to estimate the proportion of the population who knows the International symbol which we are talking about and will give a representative opinion on radioactivity and will indicate the information means with a better acceptation. First of all we proceeded to the elaboration of a questionnaire which were presented to the required number of persons in order to get reliable results. Such a ...

183

Quality assurance program requirements (design and construction)  

International Nuclear Information System (INIS)

Appendix B to 10 CFR Part 50 establishes overall quality assurance requirements for the design, construction and operation of safety-related structures, systems, and components. This guide presents a method acceptable to the Commission for complying with these regulations with regard to overall quality assurance program requirements during design and construction of nuclear power plants. Input to this guide has been provided by the Advisory Committee on Reactor Safeguards.

184

News from Smithers Rapra  

Wastenet

...of Mixing Sections 9 Interacting Rotor/Stator Mixers 10 Floating Ring Mixing Devices 11 Static (or Motionless) Mixers 12 Incorporation of Liquid Additives and Dispersions by Direct Addition 13 Dispersive Mixing of Fillers and Pigments 14 Dispersive Mixing Applied to Polymer Blending 15 Compounding with Single Screw Extruders Appendix - Preparation of Microtome Sections for Assessment of Dispersive In order to get a feel for this book, you can view Chapter 1 now, simply follow the link ...

185

Micro borehole drilling platform  

Energy Technology Data Exchange (ETDEWEB)

This study by CTES, L.C. meets two main objectives. First, evaluate the feasibility of using coiled tubing (CT) to drill 1.0 inches-2.5 inches diameter directional holes in hard rocks. Second, develop a conceptual design for a micro borehole drilling platform (MBDP) meeting specific size, weight, and performance requirements. The Statement of Work (SOW) in Appendix A contains detailed specifications for the feasibility study and conceptual design.

1996-10-01

186

Extensions of two Chow stability criteria to positive characteristics  

CERN Document Server

We extend two results on Chow (semi-)stability to positive characteristics. One is on the stability of non-singular projective hypersurfaces of degree greater than 2, and the other is the criterion by Y. Lee in terms of log canonical thresholds. Some properties of log-canonicity in positive characteristics are discussed with a couple of examples, in connection with the proof of the latter one. It is also proven in appendix that the sum of Chow (semi-)stable cycles are again Chow (semi-)stable.

2010-01-01

187

EFFECT OF SHIP ATTITUDE AND SHIP MOTION ON PRIMARY COOLANT SYSTEM FLOW RATES. Appendix A: DERIVATION OF EFFECT OF ANGULAR ACCELERATION ON DRIVING HEAD IN A NATURAL CIRCULATION REACTOR  

Science.gov (United States)

Analytical techniques for analyzing the effects of ship motion and attitude on the primary coolant system flow rates are presented. Design data for minimizing these effects are given. (C.J.G.)

1960-01-24

188

Data summary of municipal solid waste management alternatives  

Energy Technology Data Exchange (ETDEWEB)

This appendix contains the alphabetically indexed bibliography for the complete group of reports on municipal waste management alternatives. The references are listed for each of the following topics: mass burn technologies, RDF technologies, fluidized-bed combustion, pyrolysis and gasification of MSW, materials recovery- recycling technologies, sanitary landfills, composting, and anaerobic digestion of MSW.

1992-10-01

189

Chalk Point Cooling Tower Project: Cooling Tower Effects on Crops and Soils. Preoperational Report, Appendix.  

Science.gov (United States)

This report contains data collected on a monthly basis over the period April, 1973 - April, 1975 for dustfall particulates, sodium and chloride and SO2 levels obtained from 12 monitoring sites near the Chalk Point Generating Station operated by PEPCO and ...

1976-01-01

190

CRC handbook of biological effects of electromagnetic fields  

Energy Technology Data Exchange (ETDEWEB)

This book presents the current knowledge about the effects of electromagnetic fields on living matter. The three-part format covers dielectric permittivity and electrical conductivity of biological materials; effects of direct current and low frequency fields; and effects of radio frequency (including microwave) fields. The parts are designed to be consulted independently or in sequence, depending upon the needs of the reader. Useful appendixes on measurement units and safety standards are also included.

1986-01-01

191

49 CFR Appendix D to Part 230 - Civil Penalty Schedule  

Science.gov (United States)

... 2,000 230.102Tender plain bearing journal boxes: Failure to repair 1,000 1,500 230.103Tender roller bearing journal boxes: Failure to properly maintain 1,000 1,500 230.104Driving box shoes and wedges:...

2010-10-01

192

32 CFR Appendix D to Subpart M of... - Unauthorized Activities in Maneuver Training Areas  

Science.gov (United States)

...DEPARTMENT OF THE ARMY MILITARY RESERVATIONS AND...REGULATIONS AFFECTING MILITARY RESERVATIONS ...or guide service, dog training for reimbursement...non-profit organizations working in the public...profit, including dog training for reimbursement...non-profit organizations working in the public...

2010-07-01

193

Rapid preparation of pyrogen-free 2H2(18)O for human-nutrition studies  

International Nuclear Information System (INIS)

We describe a compact ultrafiltration system for the removal of pyrogens and bacteria from water labeled with the stable isotopes of deuterium and oxygen-18. The ultrafiltration system is constructed from readily available commercial components and can achieve complete removal of pyrogens and bacteria from 1L contaminated water within 30 min. By use of our procedure, loss of the isotopically labeled water by retention in the filtration system was minimal. The purified water is suitable for both oral and intravenous administration to healthy human subjects participating in nutrition studies.

194

One-dimensional free boundary problem for actin-based propulsion of Listeria  

Science.gov (United States)

Some bacteria move inside cells by recruiting the actin filaments of the host cells. The filaments are polymerized at the back surface of the bacteria, and they move away, forming a "comet" tail behind the bacterium, which consists of gel network. We develop a one-dimensional mathematical model of the gel based on partial differential equations which involve the number of filaments, the density and velocity of the gel, and the pressure. The two end-points of the gel form two free boundaries. The resulting free boundary problem is rather non-standard. We prove local existence and uniqueness.

2007-04-01

195

Effect of microwaves on microorganisms in foods  

Energy Technology Data Exchange (ETDEWEB)

The microbial safety of foods cooked in microwave ovens was investigated. The mechanisms of microwave destruction of microorganisms were examined. Effects of time and temperature on microorganisms in different food systems were described. Studies showed that: microwave heating of food is more ''''food dependent'' than conventional heating; recommended microwave treatment time for some foods may not destroy high levels of bacteria; use of microwaves in combination with conventional heating methods results in more uniform heating of foods and destruction of bacteria; and microwaves exert different killing effects on individual bacterial species. (78 references, 2 tables)

1980-08-01

196

Characterisation of anti-Staphylococcus aureus activity of quercetin  

British Library Electronic Table of Contents (United Kingdom)

Summary Although many antibiotics are available for the treatment of bacterial infections, the emergence and global spread of antibiotic-resistant bacteria is a community-wide problem. To overcome this problem, we must explore alternative antimicrobials. This study investigated the antibacterial properties of quercetin, a flavonoid present in vegetables and fruits. Quercetin was tested against gram-positive and gram-negative bacteria and was found to exert selective antibacterial activity against Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), and Staphylococcus epidermidis. Some clinical MRSA strains showed remarkable susceptibility to quercetin. In combination with antibiotics, such as oxacillin, ampicillin, vancomycin, gentamicin, and erythromycin, quercetin sho...

2010-01-01

197

C:\\Data\\My Documents\\NDA 21228 Peds web final final final Lisa  

Science.gov (United States)

... Increased frequency of psychiatric/behavioral disorders, including aggressive behavior, seen in children treated with tolterodine. ...

200

U.V. repair in deep-sea bacteria  

International Nuclear Information System (INIS)

Exposure of cells to light of less than 320 nanometers wavelengths may lead to lethal lesions and perhaps carcinogenesis. Many organisms have evolved mechanisms to repair U.V. light-induced damage. Organisms such as deep-sea bacteria are presumably never exposed to U.V. light and perhaps occasionally to visible from bioluminescence. Thus, the repair of U.V. damage in deep-sea bacterial DNA might be inefficient and repair by photoreactivation unlikely. The bacteria utilized in this investigation are temperature sensitive and barophilic. Four deep-sea isolates were chosen for this study: PE-36 from 3584 m, CNPT-3 from 5782 m, HS-34 from 5682 m, and MT-41 from 10,476 m, all are from the North Pacific ocean. The deep-sea extends from 1100 m to depths greater than 7000 m. It is a region of relatively uniform conditions. The temperature ranges from 5 to -1"0C. There is no solar light in the deep-sea. Deep-sea bacteria are ...

1986-06-08

201

Interaction of aerobic soil bacteria with plutonium(VI)  

Energy Technology Data Exchange (ETDEWEB)

We studied the interaction of Pu(VI) with Pseudomonas stutzeri ATCC 17588 and Bacillus sphaericus ATCC 14577, representatives of the main aerobic groups of soil bacteria present in the upper soil layers. The accumulation studies have shown that these soil bacteria accumulate high amounts of Pu(VI). The sorption efficiency toward Pu(VI) decreased with increasing biomass concentration due to increased agglomeration of the bacteria resulting in a decreased total surface area and number of available complexing groups. Spores of Bacillus sphaericus showed a higher biosorption than the vegetative cells at low biomass concentration which decreased significantly with increasing biomass concentration. At higher biomass concentrations (> 0.7 g/L), the vegetative cells of both strains and the spores of B. sphaericus showed comparable sorption efficiencies. Investigations on the pH dependency of the biosorption and extraction ...

2000-08-22

202

Improved oil recovery using bacteria isolated from North Sea petroleum reservoirs  

Energy Technology Data Exchange (ETDEWEB)

During secondary oil recovery, water is injected into the formation to sweep out the residual oil. The injected water, however, follows the path of least resistance through the high-permeability zones, leaving oil in the low-permeability zones. Selective plugging of these their zones would divert the waterflood to the residual oil and thus increase the life of the well. Bacteria have been suggested as an alternative plugging agent to the current method of polymer injection. Starved bacteria can penetrate deeply into rock formations where they attach to the rock surfaces, and given the right nutrients can grow and produce exo-polymer, reducing the permeability of these zones. The application of microbial enhanced oil recovery has only been applied to shallow, cool, onshore fields to date. This study has focused on the ability of bacteria to enhance oil recovery offshore in the North Sea, where the environment can be ...

1995-12-31

203

A study of bacterial isolates from corneal specimens and their antibiotic resistance profile  

International Nuclear Information System (INIS)

We aim to examine the spectrum of bacteria causing corneal infections and their antibiotic susceptibility patterns. This will serve as a guideline for empiric therapy of corneal infections. We conducted the study over a period of 18 months from March 2001 through December 2002 in King Abdul-Aziz University Hospital, Riyadh, Kingdom of Saudi Arabia. Corneal specimens taken from 200 patients were inoculated directly onto different types of media. The isolates were identified and then tested against the appropriate topical or systemic antibiotics. Sixty-seven (33.5%) of the total specimens were culture positive and 133 (66.5%) were culture negative. Fourteen (7%) of these showed organisms in the Gram stained smears and correlated well with the culture reports. Of the 67 positive cultures, 53 (79.1%) were Gram-positive bacteria mostly coagulase-negative Staphylococci 29 (43.3%) followed by Streptococcus pneumoniae (S. pneumoniae) 13 (19.4%). Among ...

204

Skeletal remodeling dynamics: New approaches with imaging instrumentation  

Energy Technology Data Exchange (ETDEWEB)

This report of progress and future objectives timetable is based on an included schematic of goals and objectives and the project abstract which is included as Appendix 1. Five matters are summarized in the order of (1) novel methods of calcified bone confocal microscopy and reconstruction image analysis of decalcified beagle and human cortical bone serial sections, (2) macroscopic cross-correlation of beagle and human cortical and cancellous bone fractions with CT analysis, (3) guidance to the most radiobiologically important skeletal regions of interest with the just completed {sup 90}Sr bone tumor map from life time beagle studies, (4) deposition patterns of radioactive agents that participate in apatite crystal nucleation processes in bone and leave radiation-excited electrons trapped in bone mineral, and (5) the budget period timetable. The discovery that beta particles from {sup 166}Ho (T{sub {1/2}} =26 hr, {beta}{sub max} = 1.8 MeV) phosphonic acid bone ...

1991-12-01

205

Quarterly coal report, October--December 1997  

Energy Technology Data Exchange (ETDEWEB)

The Quarterly Coal Report (QCR) provides comprehensive information about US coal production, distribution, exports, imports, receipts, prices, consumption, and stocks to a wide audience, including Congress, Federal and State agencies, the coal industry, and the general public. Coke production, consumption, distribution, imports, and exports data are also provided. The data presented in the QCR are collected and published by the Energy Information Administration (EIA) to fulfill data collection and dissemination responsibilities. This report presents detailed quarterly data for october through December 1997 and aggregated quarterly historical data for 1991 through the third quarter of 1997. Appendix A displays, from 1991 on, detailed quarterly historical coal imports data, as specified in Section 202 of the energy Policy and Conservation Amendments Act of 1985 (Public Law 99-58). Appendix B gives selected quarterly tables converted to metric ...

1998-05-01

206

How does one develop the right quality assurance program for waste management projects?  

International Nuclear Information System (INIS)

The quality assurance requirements in use today for radioactive waste facilities, geologic repositories and hazardous waste projects were developed initially for the nuclear power plant industry, and their intent is being applied by regulations and guidance documents to radioactive and hazardous waste programs. The wording of the NRC quality assurance requirements in Appendix B of 10CFR50, the related guidance documents and the industry's ANSI/ASME NQA-1 were developed over a period of several years to address quality assurance for the design and construction of the complex and interactive systems to produce electrical power using nuclear fuel. Now, those same documents are the basis for the quality assurance requirements and guidance for waste management facilities and repositories. The intent of Appendix B of 10CFR50 and NQA-1 can easily be applied to waste projects, providing one understands and uses the intent of the requirements. This ...

1988-10-03

207

State of work for services provided by the Waste Sampling and Characterization Facility for effluent monitoring  

International Nuclear Information System (INIS)

This document defines the services the Waste Sampling and Characterization Facility (WSCF) shall provide Effluent Monitoring (EM) throughout the calendar year for analysis. The internal memo contained in Appendix A identifies the samples Em plans to submit for analysis in CY-1995. Analysis of effluent (liquid and air discharges) and environmental (air, liquid, animal, and vegetative) samples is required using standard laboratory procedures, in accordance with regulatory and control requirements. This report describes regulatory reporting requirements and WSCF services and data quality objectives.

208

Short term energy shortages. Hearings before the subcommittee on energy of the Committee on Science and Astronautics, U.S. House of Representatives, Ninety-Third Congress, First Session, May 3, 8, 17, 1973  

Science.gov (United States)

>The causes and implications of the impeading shortages of gasoline, heating oil, diesel fuel, jet fuel, and electricity were investigated at the May 3, 1973 hearings. Discussions and related data were presented on short-term fuel shortages and their effects on the electric utilfties and the present situation in the regulated energy industries and its effects on energy R&D. The statement of the national oil lobbers council is presented in an appendix. (MCW)

1973-01-01

209

Radiostrontium in milk and tap water. Appendix D  

International Nuclear Information System (INIS)

Results of monitoring milk and tap water in New York City are presented. The New York City sample is a monthly composite of pasteurized milk purchased daily at retail stores. The monthly "9"0Sr to calcium ratios for New York City since the inception of the sampling program in 1954 are presented. Samples of New York City tap water are taken daily, so that by the end of the month, approximately 100 liters have been collected. The available cesium-137 data expressed as the "1"3"7Cs to "9"0Sr ratio are also given.

1980-01-01

210

PFP Interface identification and management planning guide  

Energy Technology Data Exchange (ETDEWEB)

The purpose of-this planning guide is to present the process used to identify, document, and control PFP Stabilization and Deactivation Project interfaces. Revisions to this document will include, as attachments, the most recent version of the Project Interface Management List. A preliminary Interface Management List is included in Appendix A. This document is intended be a Project owned management tool. As such, this document will periodically require revisions resulting from improvements of the information, processes, and techniques as now described. For most revisions that suggest improved processes, PFP management approval is all that will be required.

1999-05-20

211

Material-not-categorized-as-waste survey for 1992  

Energy Technology Data Exchange (ETDEWEB)

In October 1992, the US Department of Energy, Richland Field Office (RL) requested that Westinghouse Hanford Company (WHC) respond to a letter from EM-331 asking for completion of a survey of items in storage but not categorized as waste (Roberts 1992). The letter contained an attachment with instructions on how to fill out the attached form and what to exclude from the survey (Appendix A). This report is a summary of the information from the response issued to RL. This report primarily is for use in estimating future waste volumes that may have been overlooked because of the nature of their classification as material not categorized as waste (MNCAW) (i.e., not yet declared Waste).

1993-07-01

212

Light Scalar Mesons as Manifestation of Spontaneously Broken Chiral Symmetry  

CERN Document Server

Attention is paid to the production mechanisms of light scalars that reveal their nature. We reveal the chiral shielding of the \\sigma(600) meson. We show that the kaon loop mechanism of the \\phi radiative decays, ratified by experiment, points to the four-quark nature of light scalars. We show also that the light scalars are produced in the two photon collisions via four-quark transitions in contrast to the classic P wave tensor q\\bar q mesons that are produced via two-quark transitions $\\gamma\\gamma\\to q\\bar q$. The history of spontaneous breaking of symmetry in quantum physics is discussed in Appendix.

2010-01-01

213

Case studies of hazardous-waste treatment to remove volatile organics. Volume 2  

Energy Technology Data Exchange (ETDEWEB)

Case studies are presented for treatment of refinery wastes in a pilot-scale thin-film evaporator, the removal of volatiles from industrial wastewater for two steam strippers, and the removal of semivolatiles from water by steam stripping followed by liquid-phase carbon adsorption. The report provides data on removal efficiency, air emissions, process residuals, treatment costs, and process limitations. Details on sampling and analytical procedures, quality assurance, and process data are contained in the Appendixes (Volume II).

1987-11-01

214

Case studies of hazardous-waste treatment to remove volatile organics. Volume 1  

Energy Technology Data Exchange (ETDEWEB)

Case studies are presented for treatment of refinery wastes in a pilot-scale thin-film evaporator, the removal of volatiles from industrial wastewater for two steam strippers, and the removal of semivolatiles from water by steam stripping followed by liquid-phase carbon adsorption. The report provides data on removal efficiency, air emissions, process residuals, treatment costs, and process limitations. Details on sampling and analytical procedures, quality assurance, and process data are contained in the Appendixes (Volume II).

1987-11-01

215

Thermophilic slurry-phase treatment of petroleum hydrocarbon waste sludges  

Energy Technology Data Exchange (ETDEWEB)

Chemoheterotrophic thermophilic bacteria were used to achieve enhanced hydrocarbon degradation during slurry-phase treatment of oily waste sludges from petroleum refinery operations. Aerobic and anaerobic bacterial cultures were examined under thermophilic conditions to assess the effects of mode of metabolism on the potential for petroleum hydrocarbon degradation. The study determined that both aerobic and anaerobic thermophilic bacteria are capable of growth on petroleum hydrocarbons. Thermophilic methanogenesis is feasible during the degradation of hydrocarbons when a strict anaerobic condition is achieved in a slurry bioreactor. Aerobic thermophilic bacteria achieved the largest apparent reduction in chemical oxygen demand, freon extractable oil, total and volatile solid,s and polycyclic aromatic hydrocarbons (PAHs) when treating oily waste sludges. The observed shift with time in the molecular weight distribution of ...

1995-12-31

216

Thermophilic slurry-phase treatment of petroleum hydrocarbon waste sludges  

International Nuclear Information System (INIS)

Chemoheterotrophic thermophilic bacteria were used to achieve enhanced hydrocarbon degradation during slurry-phase treatment of oily waste sludges from petroleum refinery operations. Aerobic and anaerobic bacterial cultures were examined under thermophilic conditions to assess the effects of mode of metabolism on the potential for petroleum hydrocarbon degradation. The study determined that both aerobic and anaerobic thermophilic bacteria are capable of growth on petroleum hydrocarbons. Thermophilic methanogenesis is feasible during the degradation of hydrocarbons when a strict anaerobic condition is achieved in a slurry bioreactor. Aerobic thermophilic bacteria achieved the largest apparent reduction in chemical oxygen demand, freon extractable oil, total and volatile solid,s and polycyclic aromatic hydrocarbons (PAHs) when treating oily waste sludges. The observed shift with time in the molecular weight distribution of ...

1995-04-24

217

Phenotypic characterization of human pathogenic bacteria in fish from the coastal waters of South West Cameroon: public health implications.  

Science.gov (United States)

Increasing economic and recreational opportunities, attractive scenery and a perception of a better quality of life are luring people to the coast. Unfortunately, these activities together with the commensurate increase in population in the area inevitably result in pollution of coastal waters with excessive microorganisms and other pollutants. Microbial pollutants not only contaminate the coastal water but also aquatic food sources, thus posing a health risk to consumers. Fish is a major source of protein in Cameroon, especially in the coastal areas. In this study, we investigated the microbiological quality of fish from the Limbe and Tiko beaches in South West Cameroon from May to October 2007. We isolated human pathogenic bacteria from three anatomic sites (skin, gills, intestine) of 50 fish (150 specimens) and investigated their susceptibility patterns to a battery of antibiotics. Data were analyzed statistically using chi2 with significance set at p South West ...

218

Identification of culturable and originally non-culturable endophytic bacteria isolated from shoot tip cultures of banana cv. Grand Naine  

British Library Electronic Table of Contents (United Kingdom)

In this article we describe the identification of endophytic bacteria belonging to three groups isolated from shoot tip cultures of banana cv. Grand Naine in a recent study (Thomas et?al. 2008) based on partial 16S rRNA gene sequence homology analysis. The first group included banana stocks that displayed obvious colony growth on MS based tissue culture medium during the first in?vitro passage. The second group constituted stocks that were tissue index-negative for cultivable bacteria initially but turned index-positive after a few to several (4?8) in?vitro passages while the third group formed one sub-stock that turned index-positive after about 18 passages. The organisms belonged to about 20 different genera comprising of ?, ?, ?-proteobacteria, Gram-positive firmicutes and actinobacteri...

2008-01-01

219

Growth and reproduction of nematodes isolated from tap water; Suidosui kara bunrisareta senchurui no zoshoku tokusei  

Energy Technology Data Exchange (ETDEWEB)

This paper elucidates morphological characteristics, reproduction forms and ecological characteristics of Plectus sp. and Rhabditis sp. which are most frequently detected nematodes separated from tap water. The paper also discussed experimentally establishment of cultivating conditions for these nematodes, and behavior and reproduction characteristics of nematodes under widely ranging conditions of temperatures, bacteria concentrations and pH. Plectus sp. grows in hermaphroditic individuals, while Rhabditis sp. grows only in female. An LE culture medium can cultivate nematodes that appear in tap water, and is suitable for subculture of nematodes. Conditions of temperatures from 20{degree}C to 25{degree}C, pH and inoculated concentration of bacteria at 10{sup 8} cell {center_dot} ml{sup -1} are suitable for reproduction of Plectus sp. and Rhabditis sp. Plectus sp. and Rhabditis sp. prefer mild temperatures for reproduction, cannot reproduce ...

1996-08-10

220

Effects of microwaves on cell survival at elevated temperatures  

Energy Technology Data Exchange (ETDEWEB)

Since microwaves are used in human cancer therapy, information on specific biological effects of microwaves at elevated temperatures is important. To help supply this information, we exposed mammalian cells (CHO) and bacteria (Serratia marcescens) to hyperthermal temperatures (43, 44, and 45/sup o/C for CHO and 48, 49, and 50/sup o/C for the bacteria) with and without microwave irradiation. Temperature control was maintained by a refrigeration-reheat system and high-velocity water recirculation. The 2450-MHz microwave source was operated in a pulsed mode with power density up to 500 mW/cm/sup 2/. As expected, the survival curve slopes for both cell types increased rapidly with temperature, doubling for each degree Celsius. Microwave irradiation produced no significant change in extrapolation number for either cell type. However, survival curves of CHO cells which received microwaves were steeper by a factor of 1.25 than their sham-irradiated ...

1981-12-01

221

Biocatalytic desulfurization of petroleum and middle distillates  

Energy Technology Data Exchange (ETDEWEB)

Biocatalytic Desulfurization (BDS) represents an alternative approach to the reduction of sulfur in fossil fuels. The objective is to use bacteria to selectively remove sulfur from petroleum and middle distillate fractions, without the concomitant release of carbon. Recently, bacteria have been developed which have the ability to desulfurize dibenzothiophene (DBT) and other organosulfur molecules. These bacteria are being developed for use in a biocatalyst-based desulfurization process. Analysis of preliminary conceptual engineering designs has shown that this process has the potential to complement conventional technology as a method to temper the sulfur levels in crude oil, or remove the recalcitrant sulfur in middle distillates to achieve the deep desulfurization mandated by State and Federal regulations. This paper describes the results of initial feasibility studies, sensitivity analyses and conceptual design work. ...

1993-02-01

222

CH-TRU Waste Content Codes (CH-TRUCON)  

Energy Technology Data Exchange (ETDEWEB)

The CH-TRU Waste Content Codes (CH-TRUCON) document describes the inventory of the U.S. Department of Energy (DOE) CH-TRU waste within the transportation parameters specified by the Contact-Handled Transuranic Waste Authorized Methods for Payload Control (CH-TRAMPAC). The CH-TRAMPAC defines the allowable payload for the Transuranic Package Transporter-II (TRUPACT-II) and HalfPACT packagings. This document is a catalog of TRUPACT-II and HalfPACT authorized contents and a description of the methods utilized to demonstrate compliance with the CH-TRAMPAC. A summary of currently approved content codes by site is presented in Table 1. The CH-TRAMPAC describes "shipping categories" that are assigned to each payload container. Multiple shipping categories may be assigned to a single content code. A summary of approved content codes and corresponding shipping categories is provided in Table 2, which consists of Tables 2A, 2B, and 2C. Table 2A provides a summary of approved content codes and ...

2007-08-15

223

CH-TRU Waste Content Codes (CH-TRUCON)  

Energy Technology Data Exchange (ETDEWEB)

The CH-TRU Waste Content Codes (CH-TRUCON) document describes the inventory of the U.S. Department of Energy (DOE) CH-TRU waste within the transportation parameters specified by the Contact-Handled Transuranic Waste Authorized Methods for Payload Control (CH-TRAMPAC). The CH-TRAMPAC defines the allowable payload for the Transuranic Package Transporter-II (TRUPACT-II) and HalfPACT packagings. This document is a catalog of TRUPACT-II and HalfPACT authorized contents and a description of the methods utilized to demonstrate compliance with the CH-TRAMPAC. A summary of currently approved content codes by site is presented in Table 1. The CH-TRAMPAC describes "shipping categories" that are assigned to each payload container. Multiple shipping categories may be assigned to a single content code. A summary of approved content codes and corresponding shipping categories is provided in Table 2, which consists of Tables 2A, 2B, and 2C. Table 2A provides a summary of approved content codes and ...

2007-06-15

224

CH-TRU Waste Content Codes (CH-TRUCON)  

Energy Technology Data Exchange (ETDEWEB)

The CH-TRU Waste Content Codes (CH-TRUCON) document describes the inventory of the U.S. Department of Energy (DOE) CH-TRU waste within the transportation parameters specified by the Contact-Handled Transuranic Waste Authorized Methods for Payload Control (CH-TRAMPAC). The CH-TRAMPAC defines the allowable payload for the Transuranic Package Transporter-II (TRUPACT-II) and HalfPACT packagings. This document is a catalog of TRUPACT-II and HalfPACT authorized contents and a description of the methods utilized to demonstrate compliance with the CH-TRAMPAC. A summary of currently approved content codes by site is presented in Table 1. The CH-TRAMPAC describes "shipping categories" that are assigned to each payload container. Multiple shipping categories may be assigned to a single content code. A summary of approved content codes and corresponding shipping categories is provided in Table 2, which consists of Tables 2A, 2B, and 2C. Table 2A provides a summary of approved content codes and ...

2005-06-20

225

STRUCTURE AND FORMATION OF ELLIPTICAL AND SPHEROIDAL GALAXIES  

International Nuclear Information System (INIS)

New surface photometry of all known elliptical galaxies in the Virgo cluster is combined with published data to derive composite profiles of brightness, ellipticity, position angle, isophote shape, and color over large radius ranges. These provide enough leverage to show that Sersic log I #propor to# r "1"/"n functions fit the brightness profiles I(r) of nearly all ellipticals remarkably well over large dynamic ranges. Therefore, we can confidently identify departures from these profiles that are diagnostic of galaxy formation. Two kinds of departures are seen at small radii. All 10 of our ellipticals with total absolute magnitudes M_V_T #<=# -21.66 have cuspy cores-"missing light"-at small radii. Cores are well known and naturally scoured by binary black holes (BHs) formed in dissipationless ("dry") mergers. All 17 ellipticals with -21.54 #<=# M_V_T #<=# -15.53 do not have cores. We find a new distinct component in these galaxies: all coreless ellipticals in our sample have ...

2009-05-01

226

Carbon monoxide - hydrogen combustion characteristics in severe accident containment conditions. Final report  

International Nuclear Information System (INIS)

Carbon monoxide can be produced in severe accidents from interaction of ex-vessel molten core with concrete. Depending on the particular core-melt scenario, the type of concrete and geometric factors affecting the interaction, the quantities of carbon monoxide produced can vary widely, up to several volume percent in the containment. Carbon monoxide is a combustible gas. The carbon monoxide thus produced is in addition to the hydrogen produced by metal-water reactions and by radiolysis, and represents a possibly significant contribution to the combustible gas inventory in the containment. Assessment of possible accident loads to containment thus requires knowledge of the combustion properties of both CO and H_2 in the containment atmosphere. Extensive studies have been carried out and are still continuing in the nuclear industry to assess the threat of hydrogen in a severe reactor accident. However the contribution of carbon monoxide to the combustion threat has received less ...

1994-10-19

227

Synergistic interaction of eugenol with antibiotics against Gram negative bacteria  

British Library Electronic Table of Contents (United Kingdom)

Eugenol, the principal chemical component of clove oil from Eugenia aromatica has been long known for its analgesic, local anesthetic, anti-inflammatory, and antibacterial effects. The interaction of the eugenol with ten different hydrophobic and hydrophilic antibiotics was studied against five different Gram negative bacteria. The MIC of the combination was found to decrease by a factor of 5-1000 with respect to their individual MIC. This synergy is because of the membrane damaging nature of eugenol, where 1mM of its concentration is able to damage nearly 50% of the bacterial membrane. Eugenol was also able to enhance the activities of lysozyme, Triton X-100 and SDS in damaging the bacterial cell membrane. The hydrophilic antibiotics such as vancomycin and b-lactam antibiotics which have ...

2009-01-01

228

Responses of Bark Beetle-Associated Bacteria to Host Monoterpenes and Their Relationship to Insect Life Histories  

British Library Electronic Table of Contents (United Kingdom)

Bark beetles that colonize living conifers and their microbial associates encounter constitutive and induced chemical defenses of their host. Monoterpene hydrocarbons comprise a major component of these allelochemicals, and many are antibiotic to insects, fungi, and bacteria. Some bark beetle species exhaust these defenses by killing their host through mass attacks mediated by aggregation pheromones. Others lack adult aggregation pheromones and do not engage in pheromone-mediated mass attacks, but rather have the ability to complete development within live hosts. In the former species, the larvae develop in tissue largely depleted of host terpenes, whereas in the latter exposure to these compounds persists throughout development. A substantial literature exists on how monoterpenes affect b...

2011-01-01

229

Pit lakes: their characteristics and the potential for their remediation  

Energy Technology Data Exchange (ETDEWEB)

Pit lakes form when open-pit mining operations are discontinued and dewatering ceases. The increase in open-pit metal mining since the 1970s will lead to the formation of numerous pit lakes over the next 50 years. Many of these lakes will develop acid sulfate conditions with high levels of dissolved metals. Approaches to remediation of these conditions that have been recommended include the addition of lime or other alkaline materials and the stimulation of sulfate-reducing bacteria. However, prevention rather than remediation is probably the preferable approach. Measures to prevent oxidation of mining waste and wall rocks, including measures to fill pits quickly with water, to inhibit the activity of acidophilic sulfur-oxidizing bacteria, and to promote anoxic conditions at the lake bottoms may minimize the formation of acids and dissolved metals. (orig.)

2000-10-01

230

In vitro competitive adhesion and production of antagonistic compounds by lactic acid bacteria against fish pathogens  

British Library Electronic Table of Contents (United Kingdom)

The present study describes the screening of five lactic acid bacteria (LAB) for use as probiotics based on their competitive adhesion and production of antagonistic substances against some fish pathogens. A reduction of adhesion of all pathogenic strains tested was obtained with three of the LAB strains (Lactococcus lactis subsp. lactis CLFP100, Lactococcus lactis subsp. cremoris CLFP102 and Lactobacillus curvatus CLFP150). With the exception of fish pathogens Flavobacterium psychrophilum and Renibacterium salmoninarum that were not inhibited by LAB strains, production of antagonistic compounds by all tested LAB was observed against at least one of the indicator strains. Based on mucus adhesion, competitive exclusion, and suppression of fish pathogen growth, the selected LAB strains can b...

2007-01-01

231

Immobilization of bacteria in microgel grafted onto macroporous polyethylene  

Energy Technology Data Exchange (ETDEWEB)

The development of 'Green Chemistry' requires new materials to replace the conventional organic chemistry by biological catalysts, to produce fine chemicals in an environmentally friendly manner. Microbial whole cells can be directly used as biocatalysts, providing a simple and cheap methodology since enzyme isolation and purification are avoided. High-density polyethylene (HDPE) is a very stable polymer though it can be activated by gamma radiation to induce grafting. Glycidyl methacrylate was grafted onto macroporous HDPE and PP in the range of 1-6%, proportional to the initial monomer concentration. Grafted polymers were further chemically modified with ethylenediamine to generate a cationic hydrogel of micron-size thickness onto the internal polymer surfaces. Modified polymers were able to immobilize Gram-positive and Gram-negative bacteria that can catalyze a chemical reaction as efficient as free cells do.

2010-03-15

232

How microcystin-degrading bacteria express microcystin degradation activity  

British Library Electronic Table of Contents (United Kingdom)

Abstract Alkali tolerance and the mechanism of microcystin (MC) degradation were investigated in the MC-degrading bacterial species, Sphingopyxis sp. C-1, to better understand the increased MC degradation under the alkaline conditions that arise during the disappearance of water blooms. MC-degrading bacteria harbour mlrA, mlrB and mlrC that encode MC-degrading enzymes. Sphingopyxis sp. C-1 also possesses these genes, as well as the mlrD gene that has been assumed to encode MC and its degradation transporter. This study demonstrated that MC degradation activity was promoted by the intermittent addition of microcystin-LR (MCLR) to cultures of strain C-1. That the expression of mlrA, mlrB and mlrC is induced by MCLR also was indicated, whereas that of mlrA and mlrB is induced by the MCLR degr...

2011-01-01

233

Food-grade gene expression in lactic acid bacteria  

British Library Electronic Table of Contents (United Kingdom)

Abstract In the 1990s, significant efforts were invested in the research and development of food-grade expression systems in lactic acid bacteria (LAB). At this time, Lactococcus lactis in particular was demonstrated to be an ideal cell factory for the food-grade production of recombinant proteins. Steady progress has since been made in research on LAB, including Lactococcus, Lactobacillus and Streptococcus, in the areas of recombinant enzyme production, industrial food fermentation, and gene and metabolic pathway regulation. Over the past decade, this work has also led to new approaches on chromosomal integration vectors and host/vector systems. These newly constructed food-grade gene expression systems were designed with specific attention to self-cloning strategies, food-grade selection...

2011-01-01

234

Electrochemistry of a semiconductor chalcopyrite concentrate leaching by Thiobacillus ferrooxidans  

Energy Technology Data Exchange (ETDEWEB)

Using carbon-paste-CuFeS{sub 2} electrodes and a cyclic voltammetric technique, it was found that a large number of intermediate electrochemical oxidation reactions were associated with the dissolution of chalcopyrite in presence and absence of bacteria. The effects of concentrations of copper, ferrous and ferric ions, as well as of agitation on the peaks of cyclic voltammograms were measured. It was established that chalcopyrite oxidation was solid-state controlled as suggested by the data of chronopotentiometric and chronoamperometric measurements. The activation energy of solid state diffusion of chalcopyrite leaching was determined by the Sand's method to be {triangle}E{sub a} = 20.5 kJ. The leaching mechanism is discussed in terms of solid-state properties (energy bonding) of the n-type semiconductor chalcopyrite and energy density states of redox systems of acidic bacterial leach media. A generalized model for the mechanism of chalcopyrite leaching ...

1991-01-01

235

Efficacy of three commercially available ballast water biocides against vegetative microalgae, dinoflagellate cysts and bacteria  

British Library Electronic Table of Contents (United Kingdom)

One proposed solution to the problem of ballast-mediated aquatic invasions involves chemically treating ballast water to kill key target organisms. Here, we examine the efficacy of three commercially available ballast water biocides using vegetative microalgae, dinoflagellate resting cysts and bacteria as test organisms. Chemicals tested were the ballast water biocides SeaKleen and Peraclean Ocean, and the chlorine dioxide biocide Vibrex. Results demonstrate that the applicability of each of the three chemical biocides as a routine ballast water treatment is limited by factors such as cost, biological effectiveness and possible residual toxicity of the discharged ballast water (assessed on the basis of impact on motility of vegetative marine microalgae). Of the three biocides tested, Perac...

2007-01-01

236

Comparative activity of tigecycline and tetracycline on Gram-negative and Gram-positive bacteria revealed by a multicentre study in four North European countries  

British Library Electronic Table of Contents (United Kingdom)

Abstract Background: This study involves a multicentre surveillance of tigecycline and tetracycline activity against Gram-negative and Gram-positive bacteria from primary care centres (PCCs), general hospital wards (GHWs) and intensive care units (ICUs) in Denmark (n == 9), Finland (n == 10), Norway (n == 7) and Sweden (n == 19). Methods: The hospitals were each asked to test 30 consecutive Gram-positive and 30 Gram-negative clinical isolates. Supportive information accompanying each isolate included the study centre, ward level (PCC, GHW, or ICU), patient identification and source of the isolate. Minimum inhibitory concentrations (MICs) for tetracycline and tigecycline were determined with the Etest. Results: The isolates collected comprised 1610 Gram-negative and 1767 Gram-positive clini...

2011-01-01

237

Antimicrobial silver-montmorillonite nanoparticles to prolong the shelf life of fresh fruit salad  

British Library Electronic Table of Contents (United Kingdom)

In this work, silver-montmorillonite (Ag-MMT) antimicrobial nanoparticles have been obtained by allowing silver ions from nitrate solutions to replace the Na^+ of natural montmorillonite and then to be reduced by a thermal treatment. Ag-MMT were used as active antimicrobial compounds to improve the shelf life of fresh fruit salad. In order to assess their influence on product shelf life, sensorial and microbiological quality has been monitored during the storage. The microbiological quality was determined by monitoring the principal spoilage microorganisms (mesophilic and psychrotrophic bacteria, coliforms, lactic acid bacteria, yeasts and molds). Additionally, the evolution of sensorial quality was assessed by monitoring color, odor, firmness and product overall quality. The Ag-MMT nanopa...

2011-01-01

238

A lytic enzyme cocktail from Streptomyces sp. B578 for the control of lactic and acetic acid bacteria in wine  

British Library Electronic Table of Contents (United Kingdom)

Beside yeasts, lactic acid bacteria (LAB) are the most abundant microbes in must during vinification. Whereas Oenococcos oeni is commercially used as a starter culture for the biological acid reduction in wines, other species are responsible for different types of wine spoilage. Members of the genera Pediococcus, Weissella, Leuconostoc, and Lactobacillus are producers of exopolysaccharide slimes, biogenic amines, acetic acid, and other off-flavors. In order to control microbial growth, different procedures such as heating of must and addition of sulfite or lysozyme from egg white are generally applied. Yet, because of health risks, the application of sulfite should be reduced and lysozyme is not effective against all LAB. In this study, we describe exoenzymes from a Streptomyces sp. strain...

2009-01-01

239

Vertical and Horizontal Variations in the Physiological Diversity of the Aerobic Chemoheterotrophic Bacterial Microflora in Deep Southeast Coastal Plain Subsurface Sediments  

UK PubMed Central (United Kingdom)

Aerobic chemoheterotrophic bacteria were isolated from surface soils and coastal plain subsurface (including deep aquifer) sediments (depths to 265 m) at a study site near Aiken, S.C., by plating on...Full Text Available

1989-05-01

240

Telavancin versus Standard Therapy for Treatment of Complicated Skin and Skin Structure Infections Caused by Gram-Positive Bacteria: FAST 2 Study  

UK PubMed Central (United Kingdom)

Telavancin is a bactericidal lipoglycopeptide with a multifunctional mechanism of action. We conducted a randomized, double blind, active-control phase II trial. Patients ≥18 years of age with...Full Text Available

2006-03-01

241

Staphylococcus aureus Enterotoxin D Is Secreted in Milk and Stimulates Specific Antibody Responses in Cows in the Course of Experimental Intramammary Infection  

UK PubMed Central (United Kingdom)

An enterotoxin D (SED)-producing strain of Staphylococcus aureus was used to infect one mammary gland of each of 17 lactating dairy cows. All glands became infected and shed bacteria...Full Text Available

2006-06-01

242

Radiation higienization possibility and perspectives  

International Nuclear Information System (INIS)

Food irradiation is the process of exposing food to an ionizing energy to kill harmful bacteria and other organisms and extend shelf-life. It is a safe process and has been approved by some 50 countries worldwide and applied commercially in the USA, Japan, China and several European countries for many years. Trends law regulations and new applications in Europe and another countries has been described. (author)

2005-09-07

243

Quality engineering and control. Annual progress report, January-December 1981  

International Nuclear Information System (INIS)

Brief summaries are given of research carried out in the following areas: absorption spectroscopy of titanium, niobium, and molybdenum in uranium alloys; atomic absorption spectroscopy of selenium in stainless steel, computer program improvements for spectrophotometer control, and detection of beryllium in stack gases; electrolysis for inactivation of bacteria in cooling-tower water; improved solvent analysis by gas chromatography; sequential radionuclide separation for various sample matrices; progress on an electron microprobe analyzer; and miscellaneous projects.

244

One-carbon metabolism in methanogenic bacteria: analysis of short-term fixation products of 14CO2 and 14CH3OH incorporated into whole cells.  

UK PubMed Central (United Kingdom)

Methanobacterium thermoautotrophicum, M. ruminantium, and Methanosarcina barkeri were labeled with 14CO2 (14CO2 + H14CO3- + 14CO32-) for from 2 to 45 s. Radioactivity was recovered in coenzyme M derivatives,...Full Text Available

1978-10-01

245

Moessbauer studies of the molybdenum-iron-protein in the nitrogenase of Xanthobacter autotrophicus GZ 29. Moessbaueruntersuchungen am Molybdaen-Eisen-Protein der Nitrogenase von Xanthobacter Autotrophicus GZ 29  

Energy Technology Data Exchange (ETDEWEB)

Biological cells of the nitrogen fixating bacteria Xanthobacter autotrophicus GZ 29 have been cultivated with 57-Fe, and from the nitrogenase of these cells the 57-Fe marked molybdenum-iron-protein is extracted. The Moessbauer spectra of this enzyme show that the structure of the iron cluster is similar to the structure in the molybdenum-iron-proteins of Azotobacter vinelandii, Klebsiella pneumoniae or Clostridium pasteurianum.

1983-05-01

246

Mercury Removal, Methylmercury Formation, and Sulfate-Reducing Bacteria Profiles in Wetland Mesocosms Containing Gypsum-Amended Sediments and Scirpus californicus  

Energy Technology Data Exchange (ETDEWEB)

A pilot-scale model was constructed to determine if a wetland treatment system (WTS) could effectively remove low-level mercury from an outfall located at the Department of Energy's Savannah River Site.

2001-03-02

247

Ecology and resistance of Moraxella-Acinetobacter  

International Nuclear Information System (INIS)

The diverse microenvironments of foods, changing with processing and preservation, might provide conditions that would enhance the growth of microorganisms which are the principal cause of spoilage, off-odor and unpleasant flavor in foods. Radiation is a potential process which may provide a product with far superior microbial quality for food preservation, by reduction of microbial population; elimination of food-borne pathogens; extension of shelf-life; and reduction of spoilage. The aim of irradiation at low dose level is to eliminate certain microorganisms, especially spoilage types and those of public health significance. But, the radurization dose allows the outgrowth of radioresistant bacteria. Certain strains of Moraxella-Acinetobacter (M-A) groups have been recognized as radioresistant bacteria (Welch and Maxcy, 1975), which may have gone unnoticed by food microbiologists, since these bacteria have not been ...

1977-01-01

248

Crystallization and preliminary X-ray analysis of three dUTPases from Gram-positive bacteria  

UK PubMed Central (United Kingdom)

All organisms examined to date possess a dUTPase that performs the important function of efficiently hydrolyzing dUTP to dUMP in order to prevent the incorporation of dUTP into DNA. Three putative dUTPases...Full Text Available

249

Comparative In Vitro Activity Profiles of Novel Bis-Indole Antibacterials against Gram-Positive and Gram-Negative Clinical Isolates?  

UK PubMed Central (United Kingdom)

Antimicrobial susceptibilities of 233 Gram-positive and 180 Gram-negative strains to two novel bis-indoles were evaluated. Both compounds were potent inhibitors of Gram-positive bacteria, with MIC90...Full Text Available

2010-09-01

250

Campylobacter jejuni Fatty Acid Synthase II: Structural and functional analysis of ?-hydroxyacyl-ACP dehydratase (FabZ)  

UK PubMed Central (United Kingdom)

Fatty acid biosynthesis is crucial for all living cells. In contrast to higher organisms, bacteria use a type II fatty acid synthase (FAS II) composed of a series of individual proteins, making...Full Text Available

2009-03-06

251

Biosorption for the separation of radionuclides from drainage and process waters of the uranium mining industry  

Energy Technology Data Exchange (ETDEWEB)

Biosorption means the storage of substances at the cell envelope. Different microbial biomasses were tested for the separation of radionuclides from mining waters. Results of a pilot plant demonstrate the ability of these techniques for water cleaning processes. An effluent concentration of lower than 1 mg/l (in most cases 0.1 mg/1) could be realized in a pilot plant by using pure cells of a methylotrophic strain of bacteria as well as using of a fungal mycelia.

1995-12-31

252

Department of the  

Wastenet

Waste Technology and Innovation Study Final Report ...Waste Technology and Innovation Study Final Report 21/18569/150554 ...Survey of waste and resource recovery related technology and innovation, including products and product uses

255

THE INTERPRETATION OF ELECTRON MICROSCOPE ...  

Science.gov (United States)

... Accession Number : AD0633770. Title : THE INTERPRETATION OF ELECTRON MICROSCOPE FRACTOGRAPHS. Descriptive Note : Final rept.,. ...

1966-01-21

257

Camouflage Visualization  

Science.gov (United States)

... AAMHERST SYSTEMS INC. CAMOUFLAGE VISUALIZATION FINAL REPORT ... A AMHERST SYSTEMS INC. CAMOUFLAGE VISUALIZATION ...

1992-04-07

258

UK Greenhouse Gas Emission Inventory, 1990 to 1993. Annual report for submission under the framework convention on climate change  

Energy Technology Data Exchange (ETDEWEB)

This report presents estimates of the annual anthropogenic UK emissions of greenhouse gases for the years 1990-93 for submission under the Framework Convention on Climate Change. The gases considered are carbon dioxide, methane, nitrous oxide, nitrogen oxides, carbon monoxide and non-methane volatile organic compounds. Emissions of all the gases have declined since 1990. In particular, those of carbon dioxide have fallen by 4%; those of methane by 8% and those of nitrous oxide by 28%. Emission estimates are reported in the IPCC Standard Tables format disaggregated by source category and fuel. The methodology used for the estimates and how they relate to the UK National Atmospheric Emissions Inventory is discussed in an appendix. (author)

1995-10-01

259

Northeast coal study: resource planning framework, resource development status report. 3 Vols  

Energy Technology Data Exchange (ETDEWEB)

The objective of this study was to provide a framework for land use and resource allocation decisions in the Northeast Coal Block of British Columbia. The area was divided into a number of sites, and information gathered in two main areas, 1) status of the resource base, and 2) resource management/planning/development. A map and physical description of each site is provided as well as such information as - air quality, soils, vegetation, water resources, petroleum and natural gas resources, coal resources, and gravel resources, agricultural resources, timber resources, fisheries and wildlife resources, recreatational and aesthetic (visual) resources. The appendix volume provides a summary of the institutional management framework and approval procedures for all significant resource sectors. Provincial legislation and government acts are identified.

1982-01-01

260

Nomographs for estimating the levelized cost of energy from flat-plate and concentrator photovoltaic systems  

Energy Technology Data Exchange (ETDEWEB)

A set of nomographs to estimate the levelized energy cost for photovoltaic systems are presented. These are designed to assist system designers, analysts, and planners in estimating the effects of research and development and system-design optimization on the delivered cost of energy. The nomographs are based on a refined version of the energy cost estimation equation that appears in the National Photovoltaics Program Five-Year Research Plan. The nomographs have been prepared to emphasize technologies with the potential of producing low-cost energy (<$0.40/kWh levelized in nominal terms or $0.17/kWh in 1982 dollars), and thus primarily apply to far-term or advanced intermediate photovoltaic technologies. Separate sets of nomographs are presented to encompass both flat-plate and concentrator technologies. To supplement the nomographs, a set of tabular solutions of the energy cost equation are included as an appendix.

1984-12-31

261

Monte Carlo characterization of an ytterbium-169 high dose rate brachytherapy source with analysis of statistical uncertainty  

International Nuclear Information System (INIS)

An ytterbium-169 high dose rate brachytherapy source, distinguished by an intensity-weighted average photon energy of 92.7 keV and a 32.015#+-#0.009 day half-life, is characterized in terms of the updated AAPM Task Group Report No. 43 specifications using the MCNP5 Monte Carlo computer code. In accordance with these specifications, the investigation included Monte Carlo simulations both in water and air with the in-air photon spectrum filtered to remove low-energy photons below 10 keV. TG-43 dosimetric data including S_K, D(r,#theta#), #LAMBDA#, g_L(r), F(r,#theta#), #phi#_a_n(r), and #phi#_a_n were calculated and statistical uncertainties in these parameters were derived and calculated in the appendix.

2006-01-01

262

KALIMER preliminary conceptual design report  

Energy Technology Data Exchange (ETDEWEB)

This report, which summarizes the result of preliminary conceptual design activities during Phase 1, follows the format of safety analysis report. The purpose of publishing this report is to gather all of the design information developed so far in a systematic way so that KALIMER designers have a common source of the consistent design information necessary for their future design activities. This report will be revised and updated as design changes occur and more detailed design specification is developed during Phase 2. Chapter 1 describes the KALIMER Project. Chapter 2 includes the top level design requirements of KALIMER and general plant description. Chapter 3 summarizes the design of structures, components, equipment and systems. Specific systems and safety analysis results are described in the remaining chapters. Appendix on the HCDA evaluation is attached at the end of this report.

2000-08-01

263

IEEE standard quality assurance program requirements for the design and manufacture of class 1E instrumentation and electric equipment for nuclear power generating station  

International Nuclear Information System (INIS)

The Institute of Electrical and Electronic Engineers (IEEE) standards for the quality assurance requirements for the design and manufacture of Class 1E instrumentation and electric equipment for a nuclear power generating station are presented. This standard establishes Quality Assurance Program requirements for electrical manufacturers. This standard, used in conjunction with ANSI N45.2-1977 and the supplemental standards to the extent referenced in this standard, is intended to meet the requirements of 10CFR50, Appendix B. These requirements are intended to assure that Class 1E instrumentation and electric equipment are responsibly engineered and manufactured from proper materials, that the manufacturing processes and practices are controlled, and that the manufacturing process produces a product of the required quality for Class 1E application.

264

Guidelines for establishing, maintaining, and extending the shelf life capability of limited life items (NCIG-13)  

Energy Technology Data Exchange (ETDEWEB)

Under 10CFR50 Appendix B, utilities have a commitment to establish and maintain material control programs to assure the safety and reliability of their power plants. The guidelines in this report discuss methods of establishing, maintaining, and extending the shelf life of limited life items. In addition, these guidelines establish justifiable shelf lives for a sample of 70 items and review the effects of environmental conditions. Moreover, the guidelines are consistent with utility qualification test documentation and provide for evaluation of items with expired shelf lives. These guidelines set precedent by indicating that the shelf life is greater than previously available for many items. This increase in shelf life has been established through natural and accelerated aging tests.

1992-05-01

265

Flue gas desulfurization process, study. phase I. Survey of major installations. Appendix 95-d, Sea water scrubbing flue gas desulfurization process  

Science.gov (United States)

An assessment study of the sea water scrubbing process for flue gas desulfurization has been made by SINTEF. This project has been financially supported by the Royal Ministry of Environment of Norway. The study is based on three FGD-units which are installed at three oil-fired, industrial boilers respectively with a maximum continuous heat input of 130 MW (27 MW + 2x51.5 MW) corresponding to approximately 50 MWE (10 MWE + 2x20 MWE) generating capacity for a power plant having a net efficiency of 38%.

1979-01-01

266

Erosion below submarine structures  

Energy Technology Data Exchange (ETDEWEB)

A numerical model for the development of bed dominated scour around sub-sea structures is presented. The hydrodynamic part of the model is based on a modified potential flow theory. The purpose of the present study has been to develop relatively simple models, yet complex enough to capture the main physics that can be used as engineering tools. An overview of scour phenomena and the basic problems of scour in steady current flow is presented. A hydrodynamic model for horseshoe vortex is presented, as well as a model for the bed transport, and examples are given. The scour in oscillatory flow and a curvilinear model for suspended sediment calculations are described. An advection-diffusion model (formulated in a curvilinear stretched coordinate system) has been developed and is described in an article which has been submitted for publication, a copy of the article is enclosed in Appendix E of the report. (EG)

1997-07-01

267

Economic analysis of Darrieus vertical axis wind turbine systems for the generation of utility grid electrical power. Volume II. Economic optimization model  

Science.gov (United States)

This report is part of a four-volume study of Darrieus vertical axis wind turbine (VAWT) economics. This volume describes a computer model of VAWT cost and performance factors useful for system design and optimization. The content and limitations of the model are outlined. Output data are presented to demonstrate selection of optima and to indicate sensitivity of energy cost to design parameter variations. Optimized specifications generated by this model for six point designs are summarized. These designs subsequently receive a detailed economic analysis discussed in Volume IV. An appendix is included with a FORTRAN IV listing of the model and a description of the input/output characteristics.

1979-08-01

268

Cost-effectiveness analysis for proposed effluent limitations guidelines and standards for the coastal subcategory of the oil and gas extraction point source category  

Energy Technology Data Exchange (ETDEWEB)

The analysis is submitted in support of the effluent limitations guidelines and standards for the Coastal Oil and Gas Industry. The report analyzes the cost-effectiveness of 10 regulatory options organized by three wastestreams. The document compares the total annualized cost incurred for each of the regulatory options within each wastestream to the corresponding effectiveness of that option in reducing the discharge of pollutants. Section Two discusses the cost-effectiveness methodology used and identifies the pollutants included in the analysis, presenting their toxic weighting factors and removal efficiencies. Section Three presents the results of the analysis. In Section Four, the cost-effectiveness values are compared to cost-effectiveness values for other promulgated rules. Appendix A presents data on pollutants, pollutant removals, annualized costs, and other, more detailed information.

1995-01-01

269

Appendix B : Environment report : Securing a sustainable future for Canada`s oil sands industry  

Energy Technology Data Exchange (ETDEWEB)

The oil sands industry in Canada was said to be committed to environmentally sustainable economic development, i.e. a development that recognizes the mutual dependence of a healthy environment and a healthy economy. To indicate progress toward this goal, the report pointed out the investment of millions of dollars in environmental research and tens of millions of dollars in plant equipment already made. These infrastructure investments have paid dividends in terms of energy conservation, reduction of greenhouse gas emissions, land reclamation, air quality monitoring, water conservation, and water quality and biodiversity protection. A number of recommendations governing provincial jurisdictions over regulations were made. Particular concern was expressed over fairness, and overlapping of regulations by different levels of government.

1995-12-01

270

An historical view of the pineal gland and mental disorders  

British Library Electronic Table of Contents (United Kingdom)

Since Classical Antiquity numerous authors have linked the origin of some mental disorders to physical and functional changes in the pineal gland because of its attributed role in humans as the connection between the material and the spiritual world. The pineal organ was seen as a valve-like structure that regulated the flow of animal spirits through the ventricular system, a hypothesis that took on more vigour during the Middle Ages and the Renaissance. The framework for this theory was ''the three cells of the brain'', in which the pineal gland was even called the ''appendix of thought''. The pineal gland could also be associated with the boom, during this period, of certain legends about the ''stone of folly''. But the most relevant psychopathological role of this organ arrived with Des...

2011-01-01

271

Use of DNA probes to study tetracycline resistance determinants in gram-negative bacteria from swine  

Energy Technology Data Exchange (ETDEWEB)

Specific {sup 32}P-labeled DNA probes were prepared and used to evaluate the distribution of tetracycline resistance determinants carried by gram-negative enteric bacteria isolated from pigs in 3 swine herds with different histories of antibiotic exposure. Plasmid DNA, ranging in size from 2.1 to 186 Kb, was observed in over 84% of 114 isolates studied. Two of 78 tetracycline resistant strains did not harbor plasmids. The DNA probes were isolated from plasmids pSL18, pRT29/Tn10, pBR322 and pSL106, respectively, and they represented class A, B, C and D tetracycline resistance determinants. Hybridization conditions using 0.5X SSPE at 65{degrees}C minimize cross-hybridization between the different class of tetracycline resistance genes. Cross-hybridization between class A and class C determinants could be distinguished by simultaneous comparison of the intensity of their hybridization signals. Plasmids from over 44% of the tetracycline resistant isolates did not ...

1989-01-01

272

The anaerobic digestion process  

Energy Technology Data Exchange (ETDEWEB)

The microbial process of converting organic matter into methane and carbon dioxide is so complex that anaerobic digesters have long been treated as {open_quotes}black boxes.{close_quotes} Research into this process during the past few decades has gradually unraveled this complexity, but many questions remain. The major biochemical reactions for forming methane by methanogens are largely understood, and evolutionary studies indicate that these microbes are as different from bacteria as they are from plants and animals. In anaerobic digesters, methanogens are at the terminus of a metabolic web, in which the reactions of myriads of other microbes produce a very limited range of compounds - mainly acetate, hydrogen, and formate - on which the methanogens grow and from which they form methane. {open_quotes}Interspecies hydrogen-transfer{close_quotes} and {open_quotes}interspecies formate-transfer{close_quotes} are major mechanisms by which methanogens obtain their ...

1996-01-01

273

Test of electron beam technology on Savannah River Laboratory low-activity aqueous waste for destruction of benzene, benzene derivatives, and bacteria  

Energy Technology Data Exchange (ETDEWEB)

High energy radiation was studied as a means for destroying hazardous organic chemical wastes. Tests were conducted at bench scale with a {sup 60}Co source, and at full scale (387 l/min) with a 1.5 MV electron beam source. Bench scale tests for both benzene and phenol included 32 permutations of water quality factors. For some water qualities, as much as 99.99% of benzene or 90% of phenol were removed by 775 krads of {sup 60}Co irradiation. Full scale testing for destruction of benzene in a simulated waste-water mix showed loss of 97% of benzene following an 800 krad dose and 88% following a 500 krad dose. At these loss rates, approximately 5 Mrad of electron beam irradiation is required to reduce concentrations from 100 g/l to drinking water quality (5 {mu}g/l). Since many waste streams are also inhabited by bacterial populations which may affect filtering operations, the effect of irradiation on those populations was also studied. {sup 60}Co and electron beam irradiation were both ...

1993-08-01

274

Synthesis, characterization and biological evaluation of mononuclear Co(II), Ni(II), Cu(II) and Pd(II) complexes with new N2O2 Schiff base ligands.  

Science.gov (United States)

New tetradentate N(2)O(2) donor Schiff bases and their mononuclear Co(II), Ni(II), Cu(II), and Pd(II) complexes were synthesized and characterized extensively by IR, (1)H-, (13)C-NMR, mass, ESR, conductivity measurements, elemental and thermal analysis. Specifically the magnetic and electronic spectral measurements demonstrate the octahedral structures of cobalt(II), nickel(II) complexes and square planar geometries of copper(II), palladium(II) complexes. All the ligands and complexes were screened for their in vitro antibacterial activity against two gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) and two gram-negative bacteria (Escherichia coli, Klebsiella pneumonia). In this study, Pd(II) complexes exhibited potent antibacterial activity against B. subtilis, S. aureus whereas other metal complexes also exerted good activity towards all tested strains even than standard drugs streptomycin and ampicillin. PMID:21297294

2011-02-01

275

Influence of substrates on nitrogen removal performance and microbiology of anaerobic ammonium oxidation by operating two UASB reactors fed with different substrate levels.  

Science.gov (United States)

Both ammonium and nitrite act as substrates as well as potential inhibitors of anoxic ammonium-oxidizing (Anammox) bacteria. To satisfy demand of substrates for Anammox bacteria and to prevent substrate inhibition simultaneously; two strategies, namely high or low substrate concentration, were carefully compared in the operation of two Anammox upflow anaerobic sludge blanket (UASB) reactors fed with different substrate concentrations. The reactor working at relatively low influent substrate concentration (NO(2)(-)-N, 240 mg-NL(-1)) was shown to avoid the inhibition caused by nitrite and free ammonia. Using the strategy of low substrate concentration, a record super high volumetric nitrogen removal rate of 45.24 kg-Nm(-3) day(-1) was noted after the operation of 230 days. To our knowledge, such a high value has not been reported previously. The evidence from transmission electron microscopy (TEM) showed that the morphology and ultrastructure of ...

2010-04-13

276

Gene discovery in the Acanthamoeba castellanii genome  

Energy Technology Data Exchange (ETDEWEB)

Acanthamoeba castellanii is a free-living amoeba found in soil, freshwater, and marine environments and an important predator of bacteria. Acanthamoeba castellanii is also an opportunistic pathogen of clinical interest, responsible for several distinct diseases in humans. In order to provide a genomic platform for the study of this ubiquitous and important protist, we generated a sequence survey of approximately 0.5 x coverage of the genome. The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A. castellanii to inhabit adversity of environments. Alginate lyase may provide access to bacteria within biofilms by breaking down the biofilm matrix, and polyhydroxybutyrate depolymerase may facilitate utilization of the bacterial storage compound polyhydroxybutyrate as a food source. Enzymes for the ...

2005-08-01

277

Development and application of bio-sensor. Production of ammonia sensor  

Energy Technology Data Exchange (ETDEWEB)

The objectives of this study are to make a biosensor on a trial basis which can instantaneously measure the nitrogen in wastewater, and to develop a wastewater treatment system which is capable of on-line measurement and controlling. The system provides easier operational control relating to such a high efficient treatment as the removal of nitrogen content in wastewater, serving as a solution to the eutrophication problem. It can be applied also to the analysis of fertilizer components for agriculture. Ammonia oxidizing bacteria were immobilized with cellulose acetate film, which is mounted on a diaphragm type oxygen electrode to make a sensor, and its responsibility was studied. The gradient is slow in high concentration but sharp in low concentration, and it seems possible to use it for the measurement for less than 20 ppm nitrogen concentration. The dependence of the sensor including electrodes and activity of bacteria on temperature is ...

1989-08-01

278

Antenna organization in green photosynthetic bacteria. Progress report, March 1986--February 1987  

Energy Technology Data Exchange (ETDEWEB)

This project is concerned with the structure and function of the unique antenna system found in the green photosynthetic bacteria. The antenna system in these organisms is contained within a vesicle known as a chlorosome, which is attached to the cytoplasmic side of the cell membrane. Additional antenna pigments and reaction centers are contained in integral membrane proteins. Energy absorbed by the bacteriochlorophyll c (BChl c) pigments in the chlorosome is transferred via a ``baseplate`` array of BChl a antenna pigments into the membrane and to the reaction center. This system is similar in some respects to the phycobilisome antenna system found in cyanobacteria and some types of algae, in that a membrane-associated structure absorbs light and transfers it to the membrane where conversion to chemical energy takes place. However, the overall structure, the type of pigments utilized and the nature of the proteins in these two types of membrane-associated antenna ...

1987-12-31

279

Extension of ASME VIII Division 1 design limits  

Energy Technology Data Exchange (ETDEWEB)

ASME Subcommittee 2 on materials presented a series of questions to PVRC regarding the acceptability of using the criteria of ASME Section 2, Part D, Appendix 1 for extending design limits for AISI 304 stainless steel beyond 1,500 F to 2,000 F and Alloy 800 HT from 1,650 F to 1,800 F respectively. This paper describes a project supported by PVRC to try and find an answer to this question. The project consisted of three parts. The first was a survey to determine the intent behind the wording of the ASME criteria in order to make an extrapolation of methods for setting design limits to higher temperatures. The second was a demonstration of a methodology for developing very high temperature limits, using a set of creep data for Alloy 800 HT. The third was a parametric study to evaluate the feasibility of using the minimum creep rate based deformation criterion used in the ASME Code to set strain related limits on materials showing predominantly tertiary creep. Based ...

1995-12-01

280

The effects of cefazolin on cirrhotic patients with acute variceal hemorrhage after endoscopic interventions  

British Library Electronic Table of Contents (United Kingdom)

Background The American Association for the Study of Liver Diseases (AASLD) guidelines recommend that antibiotic prophylaxis should be instituted in any patient with cirrhosis and gastrointestinal hemorrhage, and that oral norfloxacin, intravenous ciprofloxacin, and ceftriaxone are preferable. However, the antimicrobial spectrum of the first generation of cephalosporins (cefazolin) covers a wide range of bacteria species, including community-acquired strains of Escherichia coli and Klebsiella pneumoniae, but their efficacy as prophylactic antibiotics in cirrhotic patients with acute hemorrhage was seldom warranted in the literature. This study aimed to explore the effects of cefazolin on the outcome of cirrhotic patients with acute variceal hemorrhage after endoscopic interventions. Method...

2011-01-01

281

Effects of Acetate Competition, pH and Soil Structure on the Rates and Pathways of Methane Formation in Tropical Rain Forest Soils  

Science.gov (United States)

The C isotopic composition of CH4 emissions are strongly influenced by the pathway of CH4 formation. Contrary to data from other freshwater systems, soil gas and surface flux measurements made in the tropical rain forests of Puerto Rico strongly suggest that CH4 produced in these environments was derived from CO2 reduction, rather than from acetate consumption. This study explored the effects of bacterial competition for acetate, pH, and soil structure on the pathways of CH4 formation in tropical rain forest soils. Our goal was to test two principal hypotheses: (1) ferric iron-reducing bacteria out-competed methanogens for acetate, resulting in greater CO2 reduction rather than aceticlastic methanogenesis, and (2) the low pH of tropical rain forest soils favors CO2 reduction rather than aceticlastic methanogenesis. In addition, this study also investigated the effect of destroying soil aggregate structure on the pathways and rates of CH4 production. Ferric ...

2004-12-01

282

Cell cultures are more sensitive than Saccharamoyces cervisiae tests for assessing the toxicity of aquatic pollutants  

Energy Technology Data Exchange (ETDEWEB)

Cultured fish and human cells have been used as bioassay systems for the evaluation of the toxicity of aquatic pollutants. Numerous assays using bacteria and yeast have also been used for such purposes. The authors report the toxicity of aquatic pollutants (Cd, Hg, and Ni), using cell culture systems and the yeast Saccharomyces cervisiae test. Cd, Hg, and Ni were chosen as model compounds of pollutants because the related toxicity is now fairly well established.

1988-07-01

283

Antimicrobial potential of Glycyrrhiza glabra roots  

British Library Electronic Table of Contents (United Kingdom)

The present study was aimed to investigate antimicrobial potential of Glycyrrhiza glabra roots. Antimycobacterial activity of Glycyrrhiza glabra was found at 500mg/mL concentration. Bioactivity guided phytochemical analysis identified glabridin as potentially active against both Mycobacterium tuberculosis H37Ra and H37Rv strains at 29.16mg/mL concentration. It exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. Our results indicate potential use of licorice as antitubercular agent through systemic experiments and sophisticated anti-TB assay.

2008-01-01

284

Acceleration of Emergence of Bacterial Antibiotic Resistance in Connected Microenvironments.  

Science.gov (United States)

The emergence of bacterial antibiotic resistance is a growing problem, yet the variables that influence the rate of emergence of resistance are not well understood. In a microfluidic device designed to mimic naturally occurring bacterial niches, resistance of Escherichia coli to the antibiotic ciprofloxacin developed within 10 hours. Resistance emerged with as few as 100 bacteria in the initial inoculation. Whole-genome sequencing of the resistant organisms revealed that four functional single-nucleotide polymorphisms attained fixation. Knowledge about the rapid emergence of antibiotic resistance in the heterogeneous conditions within the mammalian body may be helpful in understanding the emergence of drug resistance during cancer chemotherapy. PMID:21940899

2011-09-23

285

Microbiological Comparison of Core and Groundwater Samples Collected from a Fractured Basalt Aquifier with that of Dialysis Chamber Incubated in Situ  

Energy Technology Data Exchange (ETDEWEB)

Microorganisms associated with fractured basalt core were compared to those suspended in groundwater pumped from the same well in the eastern Snake River Plain Aquifer (Idaho, USA). Two wells influenced to different degrees by a mixed-waste plume in the fractured basalt aquifer were examined. In one well, an array of dialysis cells filled with either deionized water or crushed basalt was equilibrated to compare the microorganisms collected in this fashion with those from core and groundwater samples collected in a traditional manner from the same well. Analyses were performed to characterize these samples and to provide a basis for comparison. These included total cell counts by microscopy; total biomass by phospholipid fatty acid analysis; enumerations of viable aerobic heterotrophs, groups of putative aerobic co-metabolic TCE-degraders and aerobic H2-oxidizing bacteria; mineralization of 14C- labeled acetate; and enrichments for dissimilatory iron-reducing ...

2004-04-01

286

Microbial water diversion technique-designed for near well treatment in low temperature sandstone reservoirs in the North Sea  

Energy Technology Data Exchange (ETDEWEB)

A Norwegian Research Program on Improved Oil Recovery (IOR) in North Sea reservoirs was launched in 1992. Microbial methods, applied in this context, is a part of this program. The scope, the methodological approach, and results from the three first years are presented. Water profile control, using biomass to block high permeable zones of a reservoir, has been investigated using nitrate-reducing bacteria in the injected sea water as plugging agents. Emphasis has been put on developing a process that does not have disadvantages secondary to the process itself, such as souring and impairment of the overall injectivity of the field. Data from continuous culture studies indicate that souring may successfully be mitigated by adding nitrite to the injected seawater. The morphology and size of generic-nitrate-reducing seawater bacteria have been investigated. Screening of growth-promoting nutrients has been carried out, and some sources were detected ...

1995-12-31

287

Isolation of imidacloprid degrading bacteria from industrial sites  

International Nuclear Information System (INIS)

Immidacloprid is a cyclodiene organochlorine used as an insecticide all over the world and possessing a serous environmental threat. It is mostly used for cotton insects (bollworm, aphid and white fly). For isolation of imidacloprid degrading bacteria, two soil samples were collected from industrial contaminated sites of Kala Shah Kahu district sheikupura, having ten year history of use. Soil samples were analyzed by measuring pH and electric conductivity. The isolation of imidacroprid degrading bacteria was performed by enrichment technique. Eight bacterial strains, S/sub 1-a/ S/2-2-b/ S/2-c/ S/2-d/ S/2-e/ S/sub 2-f/ and S/sub 2-g/ and S/sub e-a/ were isolated on the basis of their colony morphologies. The purified colonies were characterized morphologically, physiologically and biochemically. Gram staining was done and Gram negative strain were confirmed on MacConkey agar and Eosin Methylene Blue. Bacterial strains were also checked for ...

288

Energetics and kinetics of anaerobic aromatic and fatty acid degradation  

Science.gov (United States)

The kinetics of benzoate degradation by the anaerobic syntrophic bacterium, Syntrophus buswellii, was studied in coculture with Desulfovibrio strain G11. The threshold value for benzoate degradation was dependent on the acetate concentration with benzoate threshold values ranging from 2.4 [mu]M at 20 mM acetate to 30.0 [mu]M at 65 mM acetate. Increasing acetate concentrations also inhibited the rate of benzoate degradation with a apparent K[sub i] for acetate inhibition of 7.0 mM. Lower threshold values were obtained when nitrate rather than sulfate was the terminal electron acceptor. These data are consistent with a thermodynamic explanation for the threshold, and suggest that there is a minimum Gibbs free energy value required for the degradation of benzoate. An acetoacetyl-CoA thiolase has been isolated from Syntrophomonas wolfei; it is apparently a key enzyme controlling the synthesis of poly-B-hydroxyalkanoate from acetyl-CoA in this organism. Kinetic characterization of the ...

1992-11-16

289

Investigation of the hadronic final state in electron-proton interactions at HERA  

Energy Technology Data Exchange (ETDEWEB)

The main subject of this thesis is the study of the hardronic final state in deep-inelastic electron-proton scattering. Theexperiment described in this thesis is performed with such a high resolution that the substructure of the proton is probed by the electron beam with a resolution of less than 10{sup -15} cm. (orig./HSI).

1995-09-18

290

Final Report of ''On-the-Job Training'' on the CANDU Reactor.  

Science.gov (United States)

This is the final Report for the technical ''on-the-job traning'' for the Wolsung CANDU nuclear power plant which is the first Pressurized Heavy Water Reactor setting up in Korea. The technical ''on-the-job traning'' was established to increase the capabi...

1983-01-01

291

Energy Engineering Analysis Program, Fort Campbell, Kentucky, energy audit of dining facilities; executive summary. Final report  

Science.gov (United States)

This volume is a summary of the results of the final report of the Energy Engineering Analysis Program (EEAP) Study of 30 selected dining facilities at Fort Campbell, Kentucky.

1986-08-01

292

Drift compression and final focus of intense heavy ion beams  

Energy Technology Data Exchange (ETDEWEB)

The longitudinal and transverse dynamics of a heavy ion fusion beam during the drift compression and final focus phase is studied. A lattice design with four time-dependent magnets is described that focuses the entire beam pulse onto a single focal point with the same spot size.

2003-05-01

293

The Detailing Process Information Technology and NEC ...  

Science.gov (United States)

... Accession Number : ADB323076. Title : The Detailing Process Information Technology and NEC Utilization. Descriptive Note : Final rept. ...

2006-09-01

294

Request for an Analytical Transmission Electron Microscope.  

Science.gov (United States)

... Accession Number : ADA189111. Title : Request for an Analytical Transmission Electron Microscope. Descriptive Note : Final rept. ...

1987-10-16

295

RECEIVED  

Science.gov (United States)

with full-scale component test data, a final requirement for completing general materials selection criteria. It also should be pointed out that, ...

299

EXPLODING BRIDGEWIRE INITIATION OF CAST ...  

Science.gov (United States)

... Accession Number : AD0368940. Title : EXPLODING BRIDGEWIRE INITIATION OF CAST EXPLOSIVES. Descriptive Note : Final rept. ...

1965-12-01

300

Development of a Colony of Germfree Hamsters as a ...  

Science.gov (United States)

... Accession Number : ADA021166. Title : Development of a Colony of Germfree Hamsters as a Biomedical Resource. Descriptive Note : Final rept. ...

1976-01-07

301

Computer code development for pipe whip and impact analysis. Progress report for year 2. Load Combination Program. Volume 2  

Energy Technology Data Exchange (ETDEWEB)

A progress report is presented on development of the WIPS computer code, a special purpose code for analysis of whip and impact in nuclear power piping. The computer code and final report are expected to be complete in December 1981. This progress report is an incomplete version of the final report, representing approximately 40% of the volume of the final report. Some sections in the report are complete, some are partially complete, and others are omitted. The report is intended to inform prospective WIPS users of the procedures, theories, and documentation standards which will be used for the computer code and the final report.

1980-12-01

302

Effect of the final coking temperature on the strength properties of formed coke  

Energy Technology Data Exchange (ETDEWEB)

Effect of final temperature and duration of coking on the properties of formed coke from weakly-caking Donetsk and Kuznetsk coals was studied. An increase of the final temperature to 850 C produced significant changes in the mechanical and chemical properties of the coke. Increasing the temperature beyond this point did not affect the mechanical strength significantly, but did increase hardness, real density and electroconductivity of the coke and decrease reactivity. Increasing the period of coking at final temperatures in the 650 and 750 C range, produced insignificant hardening of the formed coke. An objective evaluation of the effect of final temperature can be performed by a series of tests which characterize the resistivity of the formed coke to impact and abrasion and the fine structure of the matter in coke. 9 references.

1985-01-01

303

VGB guidelines on the consideration of noise emission in ordering power plant equipment  

International Nuclear Information System (INIS)

The present guidelines supplement the 'VGB guidelines for the reduction of noise in thermal power plants' with indications for the ordering and quality assurance of the individual noise-producing machines and equipment of power plant operation. These guides are to simplify the commissioning and delivering for the orderer and deliverer. In addition, these guidelines are to lead to a successful cooperation of all parties in the reduction of the sound radiation. A precondition for this is an explanation of the sound technical demands which must be made on the single machines or mechanical equipment in power plant systems. A protection of the neighbourhood as well as operational personnel against noise according to the state of technology is to be achieved by a clear form of ordering and quality assurance. The ordering and assurance procedure for all aggregates in the region of the grounds of a power plant up to the high-voltage side of the machine transformers is included in these ...

304

Use of a temperature-initiated passive cooling system (TIPACS) for the modular high-temperature gas-cooled reactor cavity cooling system (RCCS)  

Energy Technology Data Exchange (ETDEWEB)

A new type of passive cooling system has been invented (Forsberg 1993): the Temperature-Initiated Passive Cooling System (TIPACS). The characteristics of the TIPACS potentially match requirements for an improved reactor-cavity-cooling system (RCCS) for the modular high-temperature gas-cooled reactor (MHTGR). This report is an initial evaluation of the TIPACS for the MHTGR with a Rankines (steam) power conversion cycle. Limited evaluations were made of applying the TIPACS to MHTGRs with reactor pressure vessel temperatures up to 450 C. These temperatures may occur in designs of Brayton cycle (gas turbine) and process heat MHTGRs. The report is structured as follows. Section 2 describes the containment cooling issues associated with the MHTGR and the requirements for such a cooling system. Section 3 describes TIPACS in nonmathematical terms. Section 4 describes TIPACS`s heat-removal capabilities. Section 5 analyzes the operation of the temperature-control mechanism that determines under ...

1994-04-01

305

Updated TRAC analysis of an 80% double-ended cold-leg break for the AP600 design  

Energy Technology Data Exchange (ETDEWEB)

An updated TRAC 80% large-break loss-of-coolant accident (LBLOCA) has been calculated for the Westinghouse AP600 advanced reactor design, The updated calculation incorporates major code error corrections, model corrections, and plant design changes. The 80% break size was calculated by Westinghouse to be the most severe large-break size for the AP600 design. The LBLOCA transient was calculated to 144 s. Peak cladding temperatures (PCTS) were well below the Appendix K limit of 1,478 K (2,200 F), but very near the cladding oxidation temperature of 1,200 K (1,700 F). Transient event times and PCT for the TRAC calculation were in reasonable agreement with those calculated by Westinghouse using their {und W}COBRA/TRAC code. However, there were significant differences in the detailed phenomena calculated by the two codes, particularly during the blowdown phase. The reasons for these differences are still being investigated. Additional break sizes and break locations need ...

1995-07-01

306

The Development of an Audio Computer-Based Classroom Test of ESL Listening Skills.  

Science.gov (United States)

There are very few examples of audio-based computerized tests, but for many disciplines, such as foreign language and music, there appear to be many benefits to this type of testing. The purpose of the present study was to develop and compare computer-delivered and audiocassette/paper-and-pencil versions of a listening test. The test was a measure of progress achievement of academic listening comprehension and vocabulary for high-intermediate level students of English as a Second Language (ESL) at a university-affiliated institute. The underlying assumption investigated was that the use of computer and audio technology for classroom progress tests would provide benefits of convenience and improved sound quality while providing measurement quality and validity that were at least comparable to the paper form of such tests. Results with 28 students indicate that the computerized test performed at least as well as the paper-and-pencil version, with generally comparable validity. ...

1999-04-01

307

Summary of comments received from workshops on radiological criteria for decommissioning  

Energy Technology Data Exchange (ETDEWEB)

The Nuclear Regulatory Commission (NRC) is conducting an enhanced participatory rulemaking to establish radiological criteria for site cleanup and decommissioning of NRC-licensed facilities. Open public meetings were held during 1993 in Chicago, IL, San Francisco, CA, Boston, MA, Dallas, TX, Philadelphia, PA, Atlanta, GA, and Washington, DC. Interested parties were invited to provide input on the rulemaking issues before the NRC staff develops a draft proposed rule. This report summarizes 3,635 comments categorized from transcripts of the seven workshops and 1,677 comments from 100 NRC docketed letters from individuals and organizations. No analysis or response to the comments is included. The comments reflect a broad spectrum of viewpoints on the issues related to radiological criteria for site cleanup and decommissioning. The NRC also held public meetings on the scope of the Generic Environmental Impact Statement (GEIS) during July 1993. The GEIS meetings were held in Washington, ...

1994-01-01

308

Respect distances. Rationale and means of computation  

Energy Technology Data Exchange (ETDEWEB)

Canisters with spent nuclear fuel can obviously not be located within deformation zones as this might jeopardise their long term mechanical stability and thereby constitute a potential hazard to the biosphere. Less apparent, but equally important, is the fact that earthquakes trigger reactivation, slip, of structures some distance from their hypocentres due to, among many other factors, stress redistribution. Fault slip across a deposition hole might damage the isolation capacity of the canister and thereby jeopardise the overall integrity of the barrier system. Therefore, the following question might be posed: What is the distance from a deformation zone beyond which a canister can be safely emplaced? This respect distance cannot be readily computed because, unknown future events aside, there are some complicated aspects that need to be addressed e.g. degree of conservatism, scale, our ability to model ice sheets and earthquakes, etc. In this report we discuss various aspects of the ...

2004-12-01

309

Problems of waste disposal in the handling of natural radioactive material not subject to authorisation  

International Nuclear Information System (INIS)

With the exception of some minerals, the specific activity of natural radioactive material is below 370 Bq/g, their handling thus not being subject to authorization or the duty of reporting according to section 4 para. 2 of the Radiological Protection Ordinance. Moreover, there is required no handling license for prospecting, mining and processing of radioactive minerals; they are subject to the procedures for the plans of operation of the mining laws of the Federal States. License-free handling of natural radioactive material may result in the production of radioactive waste, the disposal of which must be authorized according to sect. 47 of the Radiological Protection Ordinance if the specific activity exceeds the free amounts of column 4 in Table IV of Appendix IV by a factor of 10"-"4/g. This inconsistency is discussed by means of the following examples: (1) fly ash and slag from coal power plants, (2) flue dust from sintering plants, (3) red mud from aluminium ...

1980-05-01

310

Long-term survivability of riprap for armoring uranium-mill tailings and covers: a literature review. [203 references  

Energy Technology Data Exchange (ETDEWEB)

Pacific Northwest Laboratory (PNL) is investigating the use of a rock armoring blanket (riprap) to mitigate wind and water erosion of an earthen radon suppression cover applied to uranium mill tailings. Because the radon suppression cover and the tailings must remain intact for up to 1000 years or longer, the riprap must withstand natural weathering forces. This report is a review of information on rock weathering and riprap durability. Chemical and physical weathering processes, rock characteristics related to durability, climatic conditions affecting the degree and rate of weathering, and testing procedures used to measure weathering susceptibilities have been reviewed. Sampling and testing techniques, as well as analyses of physical and chemical weathering susceptibilities, are necessary to evaluate rock durability. Many potential riprap materials may not be able to survive 1000 years of weathering. Available techniques for durability testing cannot adequately predict rock ...

1982-06-01

311

Implementation plan: Quality assurance requirements: Hazardous Waste Remedial Actions Program  

International Nuclear Information System (INIS)

This document establishes the Quality Assurance (QA) Program requirements for the Hazards Waste remedial Actions Program (HASWRAP) for ensuring, with a high degree of confidence, that program objectives will be achieved as planned. The QA Program is introduced in Sect. 1. The HAZWRAP Support Contractor Office (SCO) functional organization and QA responsibilities are shown in Sect. 2. QA program requirements are contained in Sect. 3. These requiremens are pased on the American national Standard, American National Standards Institute/American Society of Mechanical Engineers NQA-1 Quality Assurance Program Requirements for Nuclear Facilities. The 18 elements defined in the standard are tailored to HAZWRAP's needs. The QA program requirements are delineated under the major headings: Quality Assurance Program, Organization, and Control of Quality;two additional program requirements, Software Quality Assurance and Problem Prevention, are included. Definitions of QA terms and the list of ...

312

Hawaii technology utilization experiment  

Science.gov (United States)

A one-year technology-transfer project involving ERDA installations and Hawaii consisted of sending teams from the Lawrence Livermore Laboratory on week-long field trips every two months to test the effectiveness of different methods of transferring technology information from federal sources to civilian clients. The team was questioned primarily on non-energy matters, and the energy questions asked related mostly to individuals or small industries. The team responed to all questions and found that a wide range of knowledge was more effective than having a sequence of experts. Hawaiians considered current major ERDA projects to be irrelevant to their needs. The team was most successful on a one-to-one basis because large groups and state agencies tend to be more policy- than action-oriented. Personal followup was considered essential. The team also learned that their visits generated ten times as many inquiries as were received unsolicited by the laboratory. Most inquiries involved ...

1976-12-08

313

Extremely low frequency electric and magnetic fields and cancer: A literature review  

Energy Technology Data Exchange (ETDEWEB)

Epidemiologic studies suggest a weak link between occupational or residential exposure to extremely low frequency electromagnetic fields and cancer, especially tumors of the central nervous and hematopoietic systems. This report discusses these studies with reference to basic research on ELF EMF bioeffects relevant to cancer-promoting mechanisms. Many of the studies suggest a possible interaction of ELF EMF with the immune system, endocrine system, or growth-regulatory signals and morphogenic movements that involve calcium flux through cell membranes. Many bioeffects have been reported only with certain specific combinations of frequency, amplitude, and orientation of the EMF signal with respect to the geomagnetic field. The possibility of signal-specific effects raises a complication in dosimetry for both epidemiologic and experimental studies since ELF EMF effects may not be directly related to field strength. The report concludes with a discussion of design considerations for ELF ...

1989-12-01

314

Enhanced CT in the diagnosis of acute appendicitis to evaluate the severity of disease. Comparison of CT findings and histological diagnosis  

Energy Technology Data Exchange (ETDEWEB)

To assess the potential of CT in evaluating the histological severity of acute appendicitis in comparison with surgical and pathological findings. The CT images of 75 patients with surgically proven appendicitis, including 10 cases of catarrhal, 34 of phlegmonous, and 31 of gangrenous appendicitis, were retrospectively analyzed for the following five CT findings: hazy periappendiceal densities, enlarged appendix, increased enhancement of the appendiceal wall, increased enhancement of the periappendiceal intestinal wall, and deficiency of the appendiceal wall. By comparing all the CT findings and the pathological severity of appendicitis (catarrhal, phlegmonous, and gangrenous), the prevalence of the five CT findings was calculated for each pathological category. Abnormal CT findings were noted in only one case of catarrhal appendicitis. Increased enhancement of the appendiceal wall was observed in all 29 cases of phlegmonous appendicitis (100%), but in only 66.7% ...

2001-08-01

315

Electric cars in the context of the environmental and energy problemacy. Elektrische auto's in het perspectief van de milieu- en energieproblematiek  

Energy Technology Data Exchange (ETDEWEB)

An analysis is given of the problems which can be solved by the use of electric-powered vehicles. A detailed comparison is made of the energy consumption and the air polluting emissions of electric and conventional vehicles, taking into account the energy consumption of the vehicles and the primary energy consumption of electric power generation and automotive fuels production. Also the air pollution, as a result of power generation and fuel production are considered. In chapter 1 an outline is given of the traffic and transportation sector in the Netherlands with special attention to data on the energy consumption and the air pollution of this sector. Based on these data the problems can be formulated for which the application of electric vehicles can be a solution. In chapter three a brief description is given of the technical specifications and characteristics of electric vehicles. Special attention is paid to the battery types. The energy and environmental aspects of electric cars ...

1992-09-01

316

Effectiveness of storage practices in mitigating aging degradation during reactor layup  

Energy Technology Data Exchange (ETDEWEB)

One of the issues identified in the US Nuclear Regulatory Commission`s Nuclear Plant Aging Research program plan is the need to understand the state of ``mothballed`` or other out-of-service equipment to ensure subsequent safe operation. Programs for proper storage and preservation of materials and components are required by NRC regulations (10 CFR 50, Appendix B). However, materials and components have been seriously degraded due to improper storage, protection, or layup, at facilities under construction as well as those with operating licenses. Pacific Northwest Laboratory has evaluated management of aging for unstarted or mothballed nuclear power plants. The investigations revealed that no uniform guidance in the industry addresses reactor layup. In each case investigated, layup was not initiated in a timely manner, primarily because of schedule uncertainty. Hence, it is reasonable to assume that this delay resulted in accelerated aging of some ...

1995-09-01

317

Development of the Regulation Concept for a Fusion Reactor  

International Nuclear Information System (INIS)

Fusion energy has been studied in many countries such as U.S., France, Japan, Korea etc. Because it would provide much more energy for a given weight of fuel than any technology currently in use, and the fuel itself (primarily deuterium) exists abundantly in the Earth's ocean. Nuclear fusion reactor uses tritium and deuterium as fuel while nuclear fission reactor uses uranium and plutonium as fuel. Besides, inherent design characteristics and driving condition of nuclear fusion reactor is different from those of nuclear fission reactor. Therefore, we cannot apply the regulation rules of nuclear fission reactor to nuclear fusion reactor without change and thus it is needed to development of the safety regulation concept which reflects the characteristics of nuclear fusion reactor. Safety regulation of nuclear fusion reactor employs deterministic approach until sufficient data are secured. However, regulation methodology of nuclear fusion reactor should be based on risk-informed ...

2010-10-01

318

Development of QA/QC technology in Korea  

International Nuclear Information System (INIS)

KAERI (Korea Advanced Energy Research Institute) has performed research to develop the fabrication technology of CANDU nuclear fuel since 1981. Based on the satisfactory results of in-pile and out-of-pile tests of prototype nuclear fuel and the outstanding performance of 48 KAERI-made nuclear fuels in Wolsung(CANDU) power reactor, Korean government decided KAERI to supply all the nuclear fuels for Wolsung from 1988. In order to guarantee the safety and performance of nuclear fuel manufactured in mass production scale, well-organized quality assurance system and appropriate quality control techniques should be established. To establish the QA system, KAERI reviewed various QA standards and decided to establish QA system based on the 10 CFR 50 Appendix B. Quality control techniques was also revised to fit the mass production even though quality inspection techniques have already been developed during research period. By applying statistical quality control techniques ...

1986-10-06

319

Deforming the Maxwell-Sim algebra  

International Nuclear Information System (INIS)

The Maxwell algebra is a noncentral extension of the Poincare algebra, in which the momentum generators no longer commute, but satisfy [P?,P?]=Z??. The charges Z?? commute with the momenta, and transform tensorially under the action of the angular momentum generators. If one constructs an action for a massive particle, invariant under these symmetries, one finds that it satisfies the equations of motion of a charged particle interacting with a constant electromagnetic field via the Lorentz force. In this paper, we explore the analogous constructions where one starts instead with the ISim subalgebra of Poincare, this being the symmetry algebra of very special relativity. It admits an analogous noncentral extension, and we find that a particle action invariant under this Maxwell-Sim algebra again describes a particle subject to the ordinary Lorentz force. One can also deform the ISim algebra to DISimb, where b is a nontrivial dimensionless parameter. We find that the motion described by ...

2010-09-15

320

Core Heat Transfer Model Validation of the TASS/SMR-S Code using the Bennett's Test  

International Nuclear Information System (INIS)

The SMART (System-integrated Modular Advanced ReacTor) which is a 330 MWt advanced integral PWR was developed by the KAERI (Korea Atomic Energy Institute) for electricity generation and seawater desalination. A thermal hydraulic evaluation and analysis of the SMART is performed by the TASS /SMR-S (Transient And Setpoint Simulation/System integrated Modular Reactor-Safety). The TASS/SMR-S code has various models reflecting the design features of the SMART such as the drift flux model, the core models (core power and core heat transfer model), the component models, and the specific models. One of the core models is the core heat transfer model. The role of this model is to calculate the heat flux and radial temperature profiles at a fuel rod surface using the relevant heat transfer correlations for all of the heat transfer modes. Also it is modeled to meet the requirements of the 10 CFR 50 appendix K EM model for the CHF (Critical Heat Flux) and post CHF conditions. ...

2010-10-01

321

Concentrations of radionuclides in terrestrial vegetation on the Hanford site of potential interest to Native Americans  

Energy Technology Data Exchange (ETDEWEB)

Concentrations of {sup 90}Sr and {sup 137}Cs in Carey`s balsamroot (Balsamorhiza careyana) and Gray`s desert parsley (Lomatium grayi) were similar to concentrations observed in other plants collected on the Hanford Site and from offsite locations surrounding the Site as part of annual Hanford Site surveillance. Observed concentrations may be attributed to historic fallout more than to Hanford Site emissions, although the observation that 200 Area plants had slightly higher concentrations of {sup 137}Cs than 100 Area plants is consistent with other monitoring data of radioactivity in soil and vegetation collected onsite. The present concentrations of {sup 90}Sr and {sup 137}Cs in balsamroot and parsley fluctuate around background levels with some of the higher observed concentrations of {sup 90}Sr found on the Fitzner/Eberhardt Arid Lands Ecology (ALE) Reserve. Analytical results and summary statistics by species and location are presented in the appendixes.

1995-03-01

322

BREEDER: a microcomputer program for financial analysis of a large-scale prototype breeder reactor  

Energy Technology Data Exchange (ETDEWEB)

This report describes a microcomputer-based, single-project financial analysis program: BREEDER. BREEDER is a user-friendly model designed to facilitate frequent and rapid analyses of the financial implications associated with alternative design and financing strategies for electric generating plants and large-scale prototype breeder (LSPB) reactors in particular. The model has proved to be a useful tool in establishing cost goals for LSPB reactors. The program is available on floppy disks for use on an IBM personal computer (or IBM look-a-like) running under PC-DOS or a Kaypro II transportable computer running under CP/M (and many other CP/M machines). The report documents version 1.5 of BREEDER and contains a user's guide. The report also includes a general overview of BREEDER, a summary of hardware requirements, a definition of all required program inputs, a description of all algorithms used in performing the construction-period and operation-period analyses, and a summary ...

1984-04-01

323

BMFT. The 'biotechnology 2000' project. Annual report 1991. BMFT. Programm Biotechnologie 2000. Jahresbericht 1991  

Energy Technology Data Exchange (ETDEWEB)

The ''biotechnology 2000'' project is supervised by the project sponsor ''biology, energy, ecology'' at Forschungszentrum Juelich on behalf of the Federal Ministry for Research and Technology. The project activities cover the development of techniques and methods, cell biology, gene structure and gene regulation, photosynthetic production processes, biological hydrogen production, synthetic biology, protein design, neurobiological research, biosystems, plant breeding, phytomedicine and plant protection, the biology of waste disposal methods, research into methods which replace animal experiments, biological safety research, technology risk assessment, and ethical aspects. A general survey introduces the promoted projects, and standardized data sheets briefly introduce the individual activities. The appendix gives the project indices, the indices of joint projects, a list of the supported ...

1992-01-01

324

Effects of human serum on bacterial competition with neutrophils for molecular oxygen.  

Science.gov (United States)

A dialyzable factor(s) in human serum is known to stimulate gonococcal oxygen consumption. Its effect on other human pathogens was investigated. A 10% serum solution increased peak O2 consumption for Escherichia coli and Staphylococcus aureus to 157% (P less than 0.05) and 199% (P less than 0.02), respectively, of their O2 consumption when suspended in Hanks balanced salt solution, compared with a 356% increase for Neisseria gonorrhoeae with serum. Dialyzed serum lacked stimulatory capacity. Bacteria, serum, and neutrophils are often incubated to evaluate neutrophil bactericidal activity. Samples of 10(8) N. gonorrhoeae, S. aureus, and E. coli turned resazurin colorless (anaerobic conditions, Eh less than -42 mV) after 7.4, 13.3, and 15.1 min, respectively. Because neutrophil formation of reactive oxygen intermediates requires ambient O2, the effect of live bacteria and serum on this process was explored. After 5 min of incubation of 10(8) N. ...

1986-06-01

325

Whole Catchment Land Cover Effects on Water Quality in the Lower Kaskaskia River Watershed  

British Library Electronic Table of Contents (United Kingdom)

Agricultural runoff is a major non-point source pollutant and is the leading impairment of streams and rivers in the USA. This study examined the effects of agricultural, forest and urban land cover on water quality at the watershed level. Forty-three catchments ranging from 12 to 50?km2 were selected based on a land cover gradient within Lower Kaskaskia River Watershed in Illinois. Grab samples were collected and analyzed for nutrients, bacteria, and total suspended solids (TSS). Forest land cover was included in six of the ten regression models produced. Four of these regression models were for base flow conditions, suggesting that forest land cover had a significant impact on base flow water quality. Urban land cover was also included in six of the regression models. However, the majori...

2011-01-01

326

The type III secretion system is involved in Escherichia coli K1 interactions with Acanthamoeba  

British Library Electronic Table of Contents (United Kingdom)

The type III secretion system among Gram-negative bacteria is known to deliver effectors into host cell to interfere with host cellular processes. The type III secretion system in Yersina, Pseudomonas and Enterohemorrhagic Escherichia coli have been well documented to be involved in the bacterial pathogenicity. The existence of type III secretion system has been demonstrated in neuropathogenic E. coli K1 strains. Here, it is observed that the deletion mutant of type III secretion system in E. coli strain EC10 exhibited defects in the invasion and intracellular survival in Acanthamoeba castellanii (a keratitis isolate) compared to its parent strain. Next, it was determined whether type III secretion system plays a role in E. coli K1 survival inside Acanthamoeba during the encystment process...

2011-01-01

327

Testing an agent-based model of bacterial cell motility: How nutrient concentration affects speed distribution  

British Library Electronic Table of Contents (United Kingdom)

We revisit a recently proposed agent-based model of active biological motion and compare its predictions with own experimental findings for the speed distribution of bacterial cells, Salmonella typhimurium. Agents move according to a stochastic dynamics and use energy stored in an internal depot for metabolism and active motion. We discuss different assumptions of how the conversion from internal to kinetic energy d(v) may depend on the actual speed, to conclude that d 2 v ? with either ? = 2 or 1 ? < 2 are promising hypotheses. To test these, we compare the model?s prediction with the speed distribution of bacteria which were obtained in media of different nutrient concentration and at different times. We find that both hypotheses are in line with the experimental observations, with ? bet...

2011-01-01

328

Studies on biosorption and toxicity of heavy metals in bacteria; Untersuchungen zur Biosorption und zur Toxizitaet von Schwermetallen auf Bakterien  

Energy Technology Data Exchange (ETDEWEB)

Biosorption behaviour of and toxic effects of heavy metals in submersed and immobilised microorganisms was studied in batches and in continuous processes in an airlift-recycle reactor. Nickel, cadmium, and zinc were used as heavy metals. (orig./VHE) [Deutsch] Am Beispiel einer Naphthalin-2-sulfonsaeure-abbauenden Kultur wurde das Biosorptionsverhalten und die toxische Wirkung von Schwermetallen auf submerse und immobilisierte Mikroorganismen im Batch-Ansatz und im kontinuierlichen Betrieb in einem Airlift-Schlaufenreaktor untersucht. Als Schwermetalle wurden Nickel, Cadmium und Zink eingesetzt. (orig./VHE)

1993-09-01

329

Structure and antimicrobial activity relationship of quaternary N-alkyl chitosan derivatives against some plant pathogens  

British Library Electronic Table of Contents (United Kingdom)

In the present work, quaternary chitosans as water-soluble compounds were prepared based on three-step process. Schiff bases were firstly synthesized by the reaction between the amino groups of chitosan with aliphatic aldehydes followed by a reduction with sodium borohydride (NaBH4) to form N-(alkyl) chitosans. N,N,N-(dimethyl alkyl) chitosans were then obtained by a reaction of chitosan containing N-butyl, N-pentyl, N-hexyl, N-heptyl, and N-octyl substituents with methyl iodide. The compounds were characterized using IR and NMR spectroscopy. Subsequent experiments were conducted to test their antimicrobial activities against the most economic plant pathogenic bacteria of crown gall disease Agrobacterium tumefaciens, soft mold disease Erwinia carotovora, fungi of grey mold Botrytis cinerea...

2010-01-01

330

Sodium-driven motor of the polar flagellum in marine bacteria Vibrio  

British Library Electronic Table of Contents (United Kingdom)

The Na+-driven bacterial flagellar motor is a molecular machine powered by an electrochemical potential gradient of sodium ions across the cytoplasmic membrane. The marine bacterium Vibrio alginolyticus has a single polar flagellum that enables it to swim in liquid. The flagellar motor contains a basal body and a stator complexes, which are composed of several proteins. PomA, PomB, MotX, and MotY are thought to be essential components of the stator that are required to generate the torque of the rotation. Several mutations have been investigated to understand the characteristics and function of the ion channel in the stator and the mechanism of its assembly around the rotor to complete the motor. In this review, we summarize recent results of the Na+-driven motor in the polar flagellum of ...

2011-01-01

331

Serological Reactivity to Borrelia burgdorferi Sensu Lato in Dogs and Horses from Distinct Areas in Romania  

British Library Electronic Table of Contents (United Kingdom)

Abstract Lyme disease is a perfect model of the complex relationship between host, vector, and the vector-borne bacteria. Both dogs and horses in Romania are exposed to infection. The aim of the present study was to assess the seroreactivity against Borrelia burgdorferi sensu lato in dogs and horses from different regions of Romania. 276 samples from dogs and 260 samples from horses located in different regions of Romania were analyzed by ELISA and IFA, respectively. The effect of several factors potentially affecting seroreactivity (location, age, gender, occupation, and vector exposition risk) was evaluated using Fisher's exact test (R 2.12.0). The overall prevalence of anti-Borrelia antibodies was 6.52% (18/276) in dogs, with a significantly higher positivity (46.15%, 6/13, p=0.0005) re...

2011-01-01

332

Sequences of versatile, broad-host-range vectors of the RK2 family.  

Energy Technology Data Exchange (ETDEWEB)

Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available.

2003-07-01

333

Self-Organization in Space and Induced by Fluctuations  

CERN Document Server

We present a simple discrete model for the non-linear spatial interaction ofdifferent kinds of ``subpopulations'' composed of identical moving entitieslike particles, bacteria, individuals, etc. The model allows to mimic a varietyof self-organized agglomeration and segregation phenomena. By relating it togame-theoretical ideas, it can be applied not only to attractive and repulsiveinteractions in physical and chemical systems, but also to the much richercombinations of positive and negative interactions found in biological andsocio-economic systems. Apart from investigating symmetric interactions relatedto a continuous increase of the ``overall success'' within the system(``self-optimization''), we will focus on cases, where fluctuations further orinduce self-organization, even though the initial conditions and theinteractions are assumed homogeneous in space (translation invariant).

2000-01-01

334

Root Hairs Play a Key Role in the Endophytic Colonization of Olive Roots by Pseudomonas spp. with Biocontrol Activity  

British Library Electronic Table of Contents (United Kingdom)

The use of indigenous bacterial root endophytes with biocontrol activity against soil-borne phytopathogens is an environmentally-friendly and ecologically-efficient action within an integrated disease management framework. The earliest steps of olive root colonization by Pseudomonas fluorescens PICF7 and Pseudomonas putida PICP2, effective biocontrol agents (BCAs) against Verticillium wilt of olive (Olea europaea L.) caused by the fungus Verticillium dahliae Kleb., are here described. A gnotobiotic study system using in vitro propagated olive plants, differential fluorescent-protein tagging of bacteria, and confocal laser scanning microscopy analysis have been successfully used to examine olive roots?Pseudomonas spp. interactions at the single-cell level. In vivo simultaneous visualization...

2011-01-01

335

Review on the immunology of European sea bass Dicentrarchus labrax  

British Library Electronic Table of Contents (United Kingdom)

European sea bass (Dicentrarchus labrax L.) is a marine species of great economic importance, particularly in Mediterranean aquaculture. However, numerous pathogenic viruses, bacteria, fungi and parasites affect the species, causing various infectious diseases and thereby leading to the most heavy losses in aquaculture production of sea bass. In this respect, knowledge on molecular and genetic mechanisms of resistance to pathogens and specific features of immune response against various infectious agents should greatly benefit the development of effective vaccines and proper vaccination strategies in marker-assisted selection of fish resistant to a range of infections. To date, genetic knowledge on sea bass immune regulatory genes responsible for resistance to pathogens is relatively poor ...

2007-01-01

336

Regulation and secretion of Xanthomonas virulence factors  

British Library Electronic Table of Contents (United Kingdom)

Abstract Plant pathogenic bacteria of the genus Xanthomonas cause a variety of diseases in economically important monocotyledonous and dicotyledonous crop plants worldwide. Successful infection and bacterial multiplication in the host tissue often depend on the virulence factors secreted including adhesins, polysaccharides, LPS and degradative enzymes. One of the key pathogenicity factors is the type III secretion system, which injects effector proteins into the host cell cytosol to manipulate plant cellular processes such as basal defense to the benefit of the pathogen. The coordinated expression of bacterial virulence factors is orchestrated by quorum-sensing pathways, multiple two-component systems and transcriptional regulators such as Clp, Zur, FhrR, HrpX and HpaR. Furthermore, virule...

2010-01-01

337

Practical applications and feasibility of efflux pump inhibitors in the clinic-A vision for applied use  

British Library Electronic Table of Contents (United Kingdom)

The world of antibiotic drug discovery and development is driven by the necessity to overcome antibiotic resistance in common Gram-positive and Gram-negative pathogens. However, the lack of Gram-negative activity among both recently approved antibiotics and compounds in the developmental pipeline is a general trend despite the fact that the plethora of covered drug targets are well-conserved across the bacterial kingdom. Such intrinsic resistance in Gram-negative bacteria is largely attributed to the activity of multidrug resistance (MDR) efflux pumps. Moreover, these pumps also play a significant role in acquired clinical resistance. Together, these considerations make efflux pumps attractive targets for inhibition in that the resultant efflux pump inhibitor (EPI)/antibiotic combination d...

2006-01-01

338

Petroleum hydrocarbons and organic chemicals in ground water -- prevention, detection and restoration: Proceedings. Ground water management: Book 17  

International Nuclear Information System (INIS)

The 1993 Petroleum Hydrocarbons Conference was comprised of 3 days of technical presentations within the following topic areas: pollution prevention and cost control; development of remediation levels; free-phase and dissolved hydrocarbon contamination management; investigation and analysis of petroleum hydrocarbons; applications of computer modeling for remediation; design and implementation of bioventing; design and implementation of air sparging; soil vapor extraction as a remediation technique; and ground water remediation using natural bacteria. In addition, more than 100 leading companies in the ground water and petroleum industries participated in the Conference Exposition in which a variety of equipment and services for preventing, detecting and remediating ground water contaminated by petroleum hydrocarbons and other organic chemicals was showcased. Individual papers have been processed separately for inclusion in the appropriate data bases.

1993-11-10

339

Odor purification by bio-function; Seibutsu kino niyoru akushujoka  

Energy Technology Data Exchange (ETDEWEB)

Odor purification process using bio-function, which is technologically called bio-deodorizing process, utilizes the function of microorganism to convert mal-odor substances to non-odor substances. It attracts attention because the operation cost of a facility is low due to non-use of chemicals and energy, and because it is environmentally friendly without the possibility of generating secondary pollution. It is being used in deodorizing at sewage treatment facilities, fertilizer plants, coating plants, and foundries in Japan, and is more widely used in Europe. The conventional deodorizing technology in Japan mostly focuses on processes of physical chemistry such as activated carbon treatment, chemical scrubbing, combustion, and masking, but bio-deodorizing process tends to replace the conventional processes. This paper explains (1) relation of mal-odor and microorganism, (2) bio-deodorizing system (solid phase system and liquid phase system), (3) points and precaution on using ...

1999-11-30

340

Microbiological quality and biophenol content of hot air-dried Thassos cv. table olives upon storage  

British Library Electronic Table of Contents (United Kingdom)

Abstract Microbiological quality and biophenol content evolution was studied in minimally processed Thassos cv table olives by hot air dehydration under mild conditions (40C, 24-h, aw-=-0.893) and storage under characteristic packaging conditions (vacuum, 100% N2 and air) at 4 and 20C over a period of 180 days. No salt was used in the production line or packaging. The undesirable microorganisms (Enterobacteriaceae, Staphylococcus aureus, Bacillus, Clostridium) were undetectable until the end of the storage period. Also, modified atmospheres prevented fungal growth at both temperatures apart from the samples stored in air, in which Penicillium and Aspergillus spp. were identified. At 20C, a coexistence of mesophilic bacteria and yeasts occurred. At 4C, yeasts were the predominant microflora...

2011-01-01

341

Laboratory tests of sludge-control additives  

Energy Technology Data Exchange (ETDEWEB)

Laboratory {open_quotes}jar{close_quotes} tests compared eleven different fuel oil and diesel fuel sludge-control additives. Factors studied included (1) ability to disperse and prevent buildup of sludge deposits on surfaces, (2) ability to protect steel from corrosion, (3) ability to inhibit growth and proliferation of bacteria, and (4) ability to disperse water. Results varied greatly, and it was found that many commercial products do not do what they claim. It is concluded that fuel retailers should not believe manufacturers` claims for their additive products, but rather should test such products themselves to be sure that the benefits of treatment are real. A simplified form of the procedure used here is proposed as one way for dealers to do such testing.

1996-07-01

342

IL-1b enhances the antibacterial activity of astrocytes by activation of NF-kB  

British Library Electronic Table of Contents (United Kingdom)

Astrocytes have important immune functions in CNS, and astrocytes stimulated by interferon-g were showed to have direct antimicrobial function. However whether astrocytes without the stimulation of cytokines have antibacterial function, and how this function is regulated are still largely unknown. In this study, we found that primary cultured astrocytes inhibited the growth of both gram-negative and gram-positive bacteria. Further more, we showed that interleukin-1b (IL-1b) enhanced the antibacterial effect in a dose-dependent manner, and the antibacterial effect of astrocytes from IL-1b receptor-deficient mice failed to be enhanced by IL-1b. IL-1b stimulated IkBa degradation, NF-kB nuclear translocation, and transactivation in astrocytes. NF-kB inhibitors blocked NF-kB activation and the ...

2010-01-01

343

Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8  

British Library Electronic Table of Contents (United Kingdom)

This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50??g/mL) reduced the population of B. cereus from >108 to 104 colony-forming units per milliliter within 30?min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.

2011-01-01

344

Flavonols and an oxychromonol from Piliostigma reticulatum  

British Library Electronic Table of Contents (United Kingdom)

The leaf extract from the plant Piliostigma reticulatum was found to exhibit antimicrobial activity against some bacteria and fungi such as Staphylococcus aureus (NCTC 6571), Escherichia coli (NCTC 10418), Bacillus subtilis (NCTC 8236), Proteus vulgaris (NCTC 4175), Aspergillus niger (ATCC 10578) and Candida albicans (ATCC 10231). Upon investigation of the chemical constituents present in the leaf extract, a total of seven compounds were isolated and their structures were unambiguously established by spectroscopic methods including HR-MS and NMR spectrometry. Four of the isolated compounds were novel, namely 6-C-methyl-2-p-hydroxyphenyloxychromonol (piliostigmol), 1, 6,8-di-C-methylquercetin-3,3prime,7-trimethyl ether, 2, 6,8-di-C-methylquercetin-3,3prime-dimethyl ether, 3 and 3prime,6,8,-...

2008-01-01

345

Enhanced inactivation of bacteria by metal-oxide nanoparticles combined with visible light irradiation  

British Library Electronic Table of Contents (United Kingdom)

AbstractBackground In recent years nano-metaloxides which easily penetrate into the cells with special interest due to their higher chemical reactivity as compared to that of similar materials in the bulk form. Of particular interest are nano-TiO2 and ZnO, which have been widely used for their bactericidal and anticancerous properties. Purpose The aim of the present study was to examine the bactericidal properties of nano-TiO2 and ZnO combined with visible light on S. aureus and S. epidermitis, known for their high prevalence in infected wounds. Study Using the technique of electron-spin resonance (ESR) coupled with spin trapping, we examined the ability of TiO2 and ZnO nanoparticle suspensions in water to produce reactive oxygen species (ROS) with and without visible light irradiation. Th...

2011-01-01

346

Electrochromic effects of charge separation in bacterial photosynthesis: theoretical models  

Energy Technology Data Exchange (ETDEWEB)

The primary charge separation in photosynthetic bacteria generates a dimeric bacteriochlorophyll (BChl) cation and a bacteriopheophytin (BPheo) anion which lie within close proximity of each other (approx. 10 A). The two radicals also lie within van der Waals contact on opposite sides of a lone BChl bridging molecule. Spectral changes in the red (Q/sub y/) band of the bridge BChls have been observed on picosecond time scales following excitation of the reaction center (RC) and have been variously attributed to the formation of a BChl anion, to a charge-transfer state, or to electrochromic effects. They present calculations here which suggest that electrochromic effects caused by the photogenerated cation and/or anion can rationalize the optical changes observed in the flash photolyses as well as in trapping experiments.

1987-07-22

347

Effective Population Size Is Positively Correlated with Levels of Adaptive Divergence among Annual Sunflowers  

British Library Electronic Table of Contents (United Kingdom)

The role of adaptation in the divergence of lineages has long been a central question in evolutionary biology, and as multilocus sequence data sets have become available for a wide range of taxa, empirical estimates of levels of adaptive molecular evolution are increasingly common. Estimates vary widely among taxa, with high levels of adaptive evolution in Drosophila, bacteria, and viruses but very little evidence of widespread adaptive evolution in hominids. Although estimates in plants are more limited, some recent work has suggested that rates of adaptive evolution in a range of plant taxa are surprisingly low and that there is little association between adaptive evolution and effective population size in contrast to patterns seen in other taxa. Here, we analyze data from 35 loci for si...

2011-01-01

348

Effect of induction of SOS response on expression of pBR322 genes and on plasmid copy number  

Energy Technology Data Exchange (ETDEWEB)

Several lines of evidence are presented that indicate that the level of tetracycline resistance of Esherichia coli strains harboring plasmid pBR322 varies according to whether the SOS system of the host bacteria has been induced. These include use of strains in which the SOS system is expressed constitutively (lexA def.), is thermoinducible (recA441) or noninducible (lexA ind-), or is highly repressed (multiple copies of lexA+). Similar induction was observed with the product of another plasmid gene, beta-lactamase. The amounts of extractable plasmid DNA were also increased by SOS induction, and we propose that the SOS-induced increases in levels of tetracycline resistance and beta-lactamase activity are due to an increased plasmid copy number.

1989-09-01

349

Eating the enemy in Crohn's disease  

British Library Electronic Table of Contents (United Kingdom)

Several old and new observations suggest the existence in Crohn's disease of a phagocytic disorder of macrophages related to impaired bactericidal activity of host cells or to the presence of invasive bacteria that have developed strategies to counteract macrophage killing. It was recently reported that disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease. Secretion of proinflammatory cytokines by CD macrophages was impaired in response to E. coli or specific Toll-like receptor agonists. In addition, major advances in the etiology of Crohn's disease came from the existence of polymorphism in NOD2 and autophagy-related susceptibility genes (ATG16L1 and IRGM) in patients and from the identification of the presence of adhere...

2010-01-01

350

Distinctive Responses of Metabolically Active Microbiota to Acidification in a Thermophilic Anaerobic Digester  

British Library Electronic Table of Contents (United Kingdom)

Acidification is one of the most common and serious problems inducing process failure in anaerobic digesters. The production of volatile fatty acids (VFAs) mainly triggers acidic shock. However, little is known about the bacteria involved in the processes of acidogenic metabolism, such as fermentation and reductive acetogenesis. Here, the metabolic responses of a methanogenic community to the acidification and resulting process deterioration were investigated using transcriptional profiling of both the 16S rRNA and formyltetrahydrofolate synthetase (FTHFS) genes. The 16S rRNA-based analyses demonstrated that the dynamic shift of bacterial populations was closely correlated with reactor performance, especially with VFA accumulation levels. The pH drop accompanied by an increase in VFAs stim...

2011-01-01

351

Detection of irradiated chicken by ESR spectroscopy of bone  

Energy Technology Data Exchange (ETDEWEB)

Ionizing radiation has been used to treat poultry to remove harmful microorganisms, mainly Salmonella, which contaminates chicken, goose and other fresh and frozen poultry. This microorganism is sensitive to low dose radiation. Thus, irradiating these foods with doses between 1 to 7 kGy results in a large reduction of bacteria. Since it is necessary to determine whether irradiation has occurred and to what extend, this work studied the signal produced by ionizing radiation within the hard crystalline matrix of chicken`s bone to establish a control method. Chicken`s drumsticks were irradiated and bones separated from flesh were lyophilized and milled. ESR spectrum was then obtained. The ESR signal increased linearly with dose over the range 0.25 to 8.0 kGy. Free radicals evaluated during 30 days after irradiation showed stable in this period. (Author).

1995-10-01

352

Detection of Free-Living Amoebae in Some Water Sources and its Control by Ultraviolet- Radiation  

International Nuclear Information System (INIS)

Among the numerous free-living amoebae (FLA) of soil and water habitats, certain species belonging to two genera Acanthamoeba and Naegleria are facultative parasites of man.They cause disease called primary amoebic meningoencephalitis where history of contact with water was recorded in most of the cases especially with Naegleria species. The present work aimed to search for presence of free-living amoebae in the water and trials for its control by Ultraviolet-radiation (UV-radiation). Samples from different water sources were examined for the presence of free-living amoebae. These samples were cultured on non-nutrient agar streaked with bacteria. Amoebae were detected and identified by means of their morphological characters. Twelve positive cases of one hundred and twenty examined samples could be detected. The positive samples were exposed to different doses of UV-radiation for different times.

353

Complete genome sequences of Brucella melitensis strains M28 and M5-90, with different virulence backgrounds.  

Science.gov (United States)

Brucella melitensis is a Gram-negative coccobacillus bacteria belonging to the Alphaproteobacteria subclass. It is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. The B. melitensis strain M5-90, a live attenuated vaccine cultured from the B. melitensis virulent strain M28, has been an effective tool to control brucellosis in goats and sheep in China. Here we report the complete genome sequences of B. melitensis M28 and M5-90, strains with different virulence backgrounds, which will serve as a valuable reference for future studies. PMID:21478357

2011-04-08

354

Competitive adsorption of binary mixture of Leptospirillum ferriphilum and Acidithiobacillus caldus onto pyrite  

British Library Electronic Table of Contents (United Kingdom)

Leptospirillum ferriphilum and Acidithiobacillus caldus are two important acidophilic microorganisms involved in iron and sulfur oxidation during bioleaching. Cell adsorption to mineral surfaces is important for the direct leaching or contact leaching of minerals. In this study, we report the competitive adsorption of binary mixtures of L. ferriphilum LF-104 and A. caldus MTH-04 onto pyrite surfaces. The Langmuir adsorption parameter (CAm) indicated that these two bacteria underwent competitive adsorption to pyrite. Real-time quantitive PCR was used to quantify the relative amounts of L. ferriphilum and A. caldus adsorbed onto the surfaces of pyrite following exposure to a mixture of these two organisms. The adsorption of L. ferriphilum was not affected by A. caldus. However, adsorption of...

2010-01-01

355

Clostridiaceae and Enterobacteriaceae as active fermenters in earthworm gut content  

British Library Electronic Table of Contents (United Kingdom)

The earthworm gut provides ideal in situ conditions for ingested heterotrophic soil bacteria capable of anaerobiosis. High amounts of mucus- and plant-derived saccharides such as glucose are abundant in the earthworm alimentary canal, and high concentrations of molecular hydrogen (H2) and organic acids in the alimentary canal are indicative of ongoing fermentations. Thus, the central objective of this study was to resolve potential links between fermentations and active fermenters in gut content of the anecic earthworm Lumbricus terrestris by 16S ribosomal RNA (rRNA)-based stable isotope probing, with [13C]glucose as a model substrate. Glucose consumption in anoxic gut content microcosms was rapid and yielded soluble organic compounds (acetate, butyrate, formate, lactate, propionate, succi...

2011-01-01

356

Chemical and microbial properties of mahyaveh, a traditional Iranian fish sauce  

British Library Electronic Table of Contents (United Kingdom)

To the best of our knowledge, this is the first report on the chemical and microbial properties of traditional Iranian fish sauce, mahyaveh. Fish sauce samples used in this study originated from five different locations in the Southern part of Iran. The pH of mahyaveh samples from different locations was in the range of 4.89-7.55 and NaCl concentration was in the range of 7.48-17.1%. The overall mean of TVB-N in all the samples tested was 3098 mg/kg. Histamine, with the overall mean of 2662 mg/kg, was found to be the main biogenic amine in the Iranian fish sauce. The high histamine content can be related to the high levels of bacterial count especially enterobacteriaceae (overall mean of 3.41 log cfu/g) and lactic acid bacteria (overall mean of 4.13 log cfu/g) in this product. Spermidine w...

2012-01-01

357

Biological treatment of wine of distilleries  

International Nuclear Information System (INIS)

The potential of the yeast Candida tropicalis and Candida guillermondii was evaluated and an isolated partnership of microorganisms of waters of the Medellin River, conformed by two bacteria and one leavening, to degrade the content of organic matter present in wine produced by the factory of Licores and Alcoholes de Antioquia (FLA) in aerobic process with biomass production. For each one of the microorganisms in study this capacity of removal in units of chemical demand of oxygen was quantified (CDO); in addition, parameters were analyzed such as yield of the biomass in relation to the removed CDO and to total reducing sugars (TRS) consumed, time of fermentation and speed of growth different dilutions from wine. Also the possible inhibition was analyzed that the present phenolic compounds in this wine can cause in the biological process of degradation.

358

Bacterial adhesion reduction on a biocompatible Si^+ ion implanted austenitic stainless steel  

British Library Electronic Table of Contents (United Kingdom)

The colonization of an implant surface by bacteria is an extremely important medical problem, which often leads to the failure of medical devices. Modern surface modification techniques, such as ion implantation, can confer to the surfaces very different properties from those of the bulk underlying material. In this work, austenitic stainless steel 316 LVM has been superficially modified by Si^+ ion implantation. The effect of surface modification on the biocompatibility and bacterial adhesion to 316 LVM stainless steel has been investigated. To this aim, human mesenchymal stem cells (hMSCs), as precursor of osteoblastic cells, and bacterial strains relevant in infections related to orthopedic implants, i.e., Staphylococcus aureus and Staphylococcus epidermidis, have been assayed. For the ...

2011-01-01

359

Assessing the effect of an antimicrobial wound dressing on biofilms  

British Library Electronic Table of Contents (United Kingdom)

ABSTRACT To date the effect of silver-containing wound dressings on biofilms, known to be present in chronic wounds, has not been determined or documented. In this current study, we aimed to determine the antimicrobial effect of a silver-containing dressing on biofilms grown in a chambered slide model. Before the addition of a wound dressing onto a 24-hour biofilm, composed of either Pseudomonas aeruginosa, Enterobacter cloacae, Staphylococcus aureus, or a mixed bacterial community, a fluorescent dye was applied. This enabled the viability of sessile bacteria to be monitored in real-time, using a rapid form of confocal laser scanning microscopy over a contact time period of 48 hours. By analyzing all the three-dimensional data generated from the confocal time-lapse sequences, 90% of all se...

2008-01-01

360

Assessing Ground Water Development Potential Using Landsat Imagery  

British Library Electronic Table of Contents (United Kingdom)

Seven villages in southeastern Kenya surround Mt. Kasigau and depend on the mountain's cloud forest for their water supply. Five of these villages have regularly experienced water shortages, and all village water supplies were contaminated with Escherichia coli bacteria. There is a need to economically find new sources of fresh ground water. Remote sensing offers a relatively quick and cost-effective way of identifying areas with high potential for ground water development. This study used spectral properties of features on Landsat remote sensing imagery to map linear features, soil types, surface moisture, and vegetation. Linear features represented geologic or geomorphologic features indicating either shallow ground water or areas of increased subsurface hydraulic conductivity. Regarding...

2010-01-01

361

Anti-Infection Dip Suggestions for the Coloplast Titan Inflatable Penile Prosthesis in the Era of the Infection Retardant Coated Implant  

British Library Electronic Table of Contents (United Kingdom)

Abstract Introduction.- Infection is the worst complication seen with inflatable penile prosthesis (IPP). Both the American Medical Systems (AMS) and Coloplast IPP have infection retardant coatings. AMS is coated at the factory with rifampicin and minocycline (InhibiZone). The Coloplast IPP has a hydrophilic coating covalently bonded to its components that will absorb any aqueous solution before implantation and provides increased surface lubricity to decrease bacterial adherence. Aim.- We tested several antibiotic dips comparing zones of inhibition (ZOI) against five commonly infecting bacteria with coated Coloplast implants. Results were compared with those ZOI created with strips of an AMS IPP precoated with InhibiZone. Methods.- Pieces of sterile Coloplast Titan IPP were dipped in (i) ...

2011-01-01

362

An Outbreak of Post-Cataract Surgery Endophthalmitis Caused by Pseudomonas aeruginosa  

British Library Electronic Table of Contents (United Kingdom)

Objective Infectious endophthalmitis is among the most serious complications of cataract surgery. Gram-negative bacteria, including Pseudomonas aeruginosa, are responsible for less than 30% of cases; however, their rapidity of infection and virulence often results in poor visual outcome despite prompt antibiotic treatment. The purpose of this study was to investigate an outbreak of post-cataract surgery P. aeruginosa endophthalmitis in India. Design Hospital-based case series. Participants Twenty patients with acute postoperative endophthalmitis who underwent cataract surgery at one of the peripheral centers of Joseph Eye Hospital, Tiruchirapalli, Tamil Nadu, India, from February 23 to April 2, 2008. Interventions Vitreous aspirates and environmental surveillance specimens were inoculated ...

2009-01-01

363

Acid mine water treatment in wetlands: an overview of an emergent technology  

Energy Technology Data Exchange (ETDEWEB)

Experimental wetlands are being constructed on mined lands in the United States as an inexpensive alternative to conventional acid mine water treatment facilities. The US Bureau of Mines is conducting an inventory of these constructed wetlands as part of a long-term evaluative study. Preliminary results, based on the 20 sites surveyed to date, indicate that the wetlands dominated by emergent species are out-performing the Sphagnum-dominated wetlands, and that much of the water treatment is accomplished by other aspects of the wetland, including bacteria, algae, amendments and other plants. Iron concentrations as high as 85 mg/l are reduced to less than 3 mg/l after flow through the constructed wetlands. Manganese is also removed, though somewhat less efficiently. 12 refs., 1 fig., 1 tab.

1987-12-31

364

Ability of a ?minimum?? microbial food web model to reproduce response patterns observed in mesocosms manipulated with N and P, glucose, and Si  

British Library Electronic Table of Contents (United Kingdom)

We compared an idealised mathematical model of the lower part of the pelagic food web to experimental data from a mesocosm experiment in which the supplies of mineral nutrients (nitrogen and phosphorous), bioavailable dissolved organic carbon (BDOC, as glucose), and silicate were manipulated. The central hypothesis of the experiment was that bacterial consumption of BDOC depends on whether the growth rate of heterotrophic bacteria is limited by organic-C or by mineral nutrients. In previous work, this hypothesis was examined qualitatively using a conceptual food web model. Here we explore the extent to which a ?simplest possible?? mathematical version of this conceptual model can reproduce the observed dynamics. The model combines algal?bacterial competition for mineral nutrients (phosphor...

2007-01-01

365

Ground temperatures surrounding a molten fuel pool  

International Nuclear Information System (INIS)

In the analysis of the consequences of a hypothetical meltdown accident in an LMFBR, it is important to estimate the final location of the molten fuel pool in the concrete and ground underlying the reactor vessel. The GROWS program and the AYER program have been developed to calculate the final location of the molten fuel pool as the culmination of the transient analysis of this unusual Stefan problem but these programs require extensive computational resources. The solution is provided to the concrete and ground temperatures surrounding the stationary fuel pool and the related heat flux from the pool to the ground surface outside the containment building. This solution can be used to estimate the final location of the fuel pool and to check the end results of the sophisticated programs.

1977-06-01

366

Final state interactions in the decays J/{psi}{yields}VPP  

Energy Technology Data Exchange (ETDEWEB)

We investigate the interplay between crossed channel final state interactions and the constraints from two-particle unitarity for the reactions J/{psi}{yields}V{pi}{pi} and VK anti K, where V is either {omega} or {phi}. Using a model where the parameters are largely constrained by other sources, we find that, although small, crossed channel final state interaction can influence the amplitudes considerably, in special areas of phase space. These results cast doubt on the inapplicability of unitarity constraints on production amplitudes as recently claimed in the literature. (orig.)

2009-09-15

367

Clean ferrous casting technology research. Final technical report, September 29, 1993--December 31, 1995  

Energy Technology Data Exchange (ETDEWEB)

This is the final report covering work performed on research into methods of attaining clean ferrous castings. In this program methods were developed to minimize the formation of inclusions in steel castings by using a variety of techniques which decreased the tendency for inclusions to form during melting, casting and solidification. In a second project, a reaction chamber was built to remove inclusions from molten steel using electromagnetic force. Finally, a thorough investigation of the causes of sand penetration defects in iron castings was completed, and a program developed which predicts the probability of penetration formation and indicates methods for avoiding it.

1996-01-31

368

The role of nitrogen fixation in intensive forestry in Canada. Part 1  

Energy Technology Data Exchange (ETDEWEB)

Intensification of forest management and harvesting will lead to increased pressures on relatively infertile forest soils with possible reductions in levels of nitrogen and organic matter. It will be necessary for foresters to manage the soils, along with trees, if they wish to maintain site productivity. Nitrogen fixation should be considered as a supplementary or alternative soil amendment method. Free-living nitrogen-fixing bacteria appear to contribute minor amounts of combined nitrogen to forest soils, but it is possible that this contribution has been underestimated. The nitrogen-fixing symbioses of higher plants with bacteria contribute up to 300 kg year of nitrogen, more than sufficient to replace potential losses of nitrogen in intensive forestry. The nitrogen-fixing process, however, requires sufficient supplies of certain elements and is sensitive to environmental factors. There are numerous examples of nitogen-fixing symbioses ...

1984-01-01

369

The association of asymptomatic bacterial vaginosis with upper genital tract infection.  

Science.gov (United States)

Objective: To determine whether asymptomatic bacterial vaginosis (BV) is associated with upper genital tract inflammation or bacterial colonization.Methods: Fifty nonpregnant women with intact uteri who planned to undergo gynecologic surgery and had no recent symptoms of vaginal infection were enrolled. We obtained a vaginal swab for Gram stain, endocervical swab for chlamydia and gonorrhea DNA probe testing, and Pipelle endometrial biopsy for aerobic and anaerobic cultures and histology. We correlated surgical findings and histology of available surgical specimens with the microbiologic results. The diagnosis of BV was made according to Speigel's criteria. Bacteria isolated from the uterus were classified as high virulence versus low virulence. Contingency tables were analyzed using the chi-square or Fisher Exact tests.Results: Twenty-one of 50 patients had BV on Gram stain, 3 had intermediate BV, and 3 had unreadable slides. Eleven patients had histologic ...

1998-07-01

370

Sudden infant death syndrome and placental disorders: the thyroid-selenium link.  

Science.gov (United States)

Placental insufficiency, inducing hypoxia-ischaemia, is considered a major cause of neuronal injury and impaired post natal development. Placental insufficiency alters the metabolism of arachidonic acid and its oxidation products. Premature labour and low-birth-weight infants are associated with reduced intrauterine blood-flow and infections of the reproductive tract. Thyroidal activity is depressed in undernutrition (placental insufficiency). Premature infants require extra vitamin C for normal tyrosine metabolism (tyrosine is the thyroxine precursor). Among the symptoms indicating infantile cretinism, which appear during 3-5 months of age are: delayed union of skull bones, torpid behaviour, slow feeding, cyanosis during feeding, excessive sleepiness, enlarged tongue, umbilical herniation, flabby musculature, short stature and delayed development. These symptoms have all been described in low-birth-weight infants and sudden infant death syndrome victims by various authors. ...

1997-04-01

371

Quality of water and time-of-travel in Bakers Creek near Clinton, Mississippi. [Bakers Creek  

Energy Technology Data Exchange (ETDEWEB)

A short-term intensive quality-of-water study was conducted during a period of generally low streamflow in Bakers Creek and its tributary, Lindsey Creek, near Clinton, Mississippi. During the September 15-18, 1980 study, dissolved oxygen concentrations in Bakers Creek were less than 5 milligrams per liter. The specific conductance, 5-day biochemical oxygen demand, nutrient concentrations, and bacteria densities in Bakers Creek decreased downstream through the study reach. The mean specific conductance decreased from 670 to 306 microhms per centimeter. The 5-day biochemical oxygen demand decreased from 19 to 2.8 milligrams per liter. The mean total nitrogen and phosphorous concentrations decreased from 10 and 7.1 to 1.0 and 0.87 milligram per litter, respectively. The maximum fecal bacteria decreased from 7200 to 400 colonies per 100 milliliter. The concentrations of mercury, iron, and manganese in a sample collected at the downstream site ...

1982-01-01

372

Production of Shiga-like toxins by Escherichia coli O157:H7 can be influenced by the neuroendocrine hormone norepinephrine.  

Science.gov (United States)

To examine whether the neuroendocrine hormone norepinephrine may influence the production of the Shiga-like toxins (SLTs), several Escherichia coli O157:H7 clinical isolates were grown in the presence or absence of norepinephrine. An in vitro culture system consisting of low (<1500 colony-forming units/ml) initial concentrations of inocula into a serum-based medium was used to more closely approximate in vivo conditions. The growth of all isolates was increased several logs in the presence of norepinephrine, as compared with the growth in controls, during a 24-hour growth period. Controls included additional dextrose as well as the use of the norepinephrine metabolite normetanephrine, which contains one more methyl group than norepinephrine and hence would serve as a better energy source for growth if the effect were solely nutritionally mediated. During the 24 hours of growth, the production of cell-associated SLT-I on a protein-equivalent basis was shown to be increased over ...

1996-10-01

373

Microbial oxidation of soluble sulfide in produced water from the Bakkeen Sands  

Energy Technology Data Exchange (ETDEWEB)

The presence of soluble sulfide in produced water results in problems for the petroleum industry due to its toxicity, odor, corrosive nature, and potential for wellbore plugging. Sulfide oxidation by indigenous nitrate-reducing bacteria (NRB) present in brine collected from wells at the Coleville Unit (CVU) in Saskatchewan, Canada, was investigated. Sulfide oxidation took place readily when nitrate and phosphate were added to brine enrichment cultures, resulting in a decrease in sulfide levels of 99-165 ppm to nondetectable levels (< 3.3 ppm). Produced water collected from a number of producing wells was screened to determine the time required for complete sulfide oxidation, in order to select candidate wells for treatment. Three wells were chosen, based on sulfide removal in 48 hours or less. These wells were treated down the backside of the annulus with a solution containing 10 mM KNO{sub 3} and 100 {mu}M NaH{sub 2}PO{sub 4}. Following a 24- to 72-hour ...

1995-12-31

374

Manganite reduction by Shewanella putrefaciens MR-4  

Energy Technology Data Exchange (ETDEWEB)

Previous studies have documented dissimilatory growth of bacteria on solid Mn{sup 4+} oxide, but Mn{sup 3+} oxides have not been previously studied; here the authors have demonstrated for the first time the bacterial reduction of manganite. Strain MR-4 of Shewanella putrefaciens was able to grow on and rapidly reduce insoluble needle-shaped crystals of synthetic manganite (MnOOH), converting them to soluble Mn{sup 2+} in the process. The rate of Mn{sup 3+} reduction was optimal at pH of 7.0 and 26 C consistent with an enzymatic reaction. In addition the rates of reduction were in proportion to the amount of manganite added, but nearly independent of the cell concentration present (e.g., cell number had only a small effect on the rate of Mn{sup 3+} reduction at early stages of growth) suggesting that surface properties were dictating the rates of metal reduction. This thesis was supported by major differences in reduction rates when Mn oxides of different surface ...

1998-11-01

375

Gamma irradiation in the control of pathogenic bacteria in refrigerated ground chicken meat; Irradiacao gama no controle de bacterias patogenicas em carne de frango refrigerada  

Energy Technology Data Exchange (ETDEWEB)

This work evaluated the effect of gamma radiation on reducing the population of Staphylococcus aureus, Escherichia coli nd Salmonella typhimurium in ground chicken breast stored under refrigeration. The experiment included a control and 4 doses of gamma radiation ( 2.0, 4.0, 6.0 and 8.0 kGy) along with 5 periods of storage under refrigeration ( 1, 7 14, 21 and 28 days). Samples of ground chicken breast were inoculated with Staphylococcus aureus (ATCC 14458), Escherichia coli (ATCC 11105) and Salmonella typhimurium (ATCC 0626), irradiated at temperatures between 4 and 8 deg C and stored under refrigeration (5 deg C) for 28 days. The increased radiation dose and period of storage under refrigeration caused a reduction of Staphylococcus aureus, Escherichia coli and Salmonella typhimurium populations in the ground chicken breast. Mean radiation D values determined for Staphylococcus aureus and Escherichia coli were 0.41 and 0.72 kGy, respectively. Gamma irradiation was an effective ...

2000-09-01

376

Development of food preservation and processing techniques by radiation  

International Nuclear Information System (INIS)

Development of food preservation and processing techniques by radiation was performed. Gamma irradiation at 2-10 kGy is considered to be an effective method to control pathogenic bacteria in species including Escherichia coli O157:H7. Gamma irradiation at 5 kGy completely eliminated pathogenic bacteria in beef. Gamma irradiation at such doses and subsequent storage at less than 4 deg C could ensure hygienic quality and prolong the microbiological shelf-life resulting from the reduction of spoilage microorganisms. Gamma irradiation on pre-rigor beef shortens the aging-period, improves tenderness and enhances the beef quality. And, a new beef processing method using gamma irradiation, such as in the low salt sausage and hygienic beef patty was developed. Safety tests of gamma-irradiated meats(beefs: 0-5 kGy; porks: 0-30 kGy) in areas such as genotoxicity, acute toxicity, four-week oral toxicity, rat hepato carcinogenesis and the anti oxidative ...

1988-04-18

377

Anaerobic thermophilic bacteria isolated from a Venezuelan oil field and its potential use in microbial improved oil recovery  

Energy Technology Data Exchange (ETDEWEB)

The objective of this work is to determine the ability of indigenous bacteria from a Venezuelan oil field to grow under reservoir conditions inside a porous media, and to produce metabolites capable of recovering residual crude oil. For this purpose, samples of formation waters from a central-eastern Venezuelan oil reservoir were enriched with different carbon sources and a mineral basal media. Formation water was used as a source of trace metals. The enrichments obtained were incubated at reservoir temperature (71{degrees}C), reservoir pressure (1,200 psi), and under anaerobic conditions for both outside and inside porous media (Berea core). Growth and metabolic activity was followed outside porous media by measuring absorbance at 660 nm, increases in pressure, and decreases in pH. Inside porous media bacterial activity was determined by visual examination of the produced waters (gas bubbles and bacterial cells). All the carbohydrates tested outside porous media ...

1995-12-31

378

Telephone Flat Geothermal Development Project Environmental Impact Statement Environmental Impact Report. Final  

Energy Technology Data Exchange (ETDEWEB)

This Final Environmental Impact Statement and Environmental Impact Report (Final EIS/EIR) has been prepared to meet the requirements of the National Environmental Policy Act (NEPA) and the California Environmental Quality Act (CEQA). The Proposed Action includes the construction, operation, and decommissioning of a 48 megawatt (gross) geothermal power plant with ancillary facilities (10-12 production well pads and 3-5 injection well pads, production and injection pipelines), access roads, and a 230-kilovolt (kV) transmission line in the Modoc National Forest in Siskiyou County, California. Alternative locations for the power plant site within a reasonable distance of the middle of the wellfield were determined to be technically feasible. Three power plant site alternatives are evaluated in the Final EIS/EIR.

1999-02-01

379

Selection, Training, and Control Problems in the Use of the ...  

Science.gov (United States)

... Title : Selection, Training, and Control Problems in the Use of the Military Working Dog. Descriptive Note : Final rept. 15 Jan 72-14 Sep 73,. ...

1974-04-02

380

Revision Knee Surgery  

Medline Plus

... flex to LCCK components, to even a rotating hinge knee arthroplasty, and finally, to the segmental system. ... it also allows you to convert to the hinge immediately too, you know, especially in these bigger ...

381

Pulmonary Function Screening of Submarine Personnel Prior ...  

Science.gov (United States)

... as the difference between date of birth and date of ... Final model selections were based on the ... approval by the Naval Submarine Medical Research ...

2007-03-26

382

Placement of Network Security Services for Secure Data ...  

Science.gov (United States)

... Accession Number : ADA298752. Title : Placement of Network Security Services for Secure Data Exchange. Descriptive Note : Final rept.,. ...

1995-09-05

383

Parameter Estimation in Moving Boundary Problems  

Science.gov (United States)

... Finally, the one dimensional, one phase Stefan problem is well known as a model for the melting of ice (see, eg, [5]). There are many approaches to ...

1988-05-01

384

Omental Lipid-Coated Mesh  

Science.gov (United States)

... Accession Number : ADA544419. Title : Omental Lipid-Coated Mesh. Descriptive Note : Final rept. 1 Jul 2009-16 Jun 2011. ...

2011-06-16

385

Nuclear power plant liquid waste solidification system. [Japan  

Energy Technology Data Exchange (ETDEWEB)

The fundamental points to be considered in a waste treatment system for a country like Japan, where the final disposal method has not been decided and the wastes have to be stored in the power plants, are volume reduction of the wastes, safe storage of the wastes in the plant, and flexibility regarding the final disposal. A system has been developed that consists of a thin film evaporator for the direct solidification of the liquid waste, a pelletizer for producing hard pellets from the powdered wastes, a pellet storage unit, and a solidification unit for the final disposal. A pilot plant with waste treatment capacity of 200 kg/h was built in 1976 and has proved the system feasibility. This paper reports on pilot plant tests of the thin film evaporator and other components, tests on pellet deterioration during long term storage, and integrity tests on the final disposal of the pellet bitumen package.

1981-01-01

386

Nuclear power plant liquid waste solidification system  

International Nuclear Information System (INIS)

The fundamental points to be considered in a waste treatment system for a country like Japan, where the final disposal method has not been decided and the wastes have to be stored in the power plants, are volume reduction of the wastes, safe storage of the wastes in the plant, and flexibility regarding the final disposal. A system has been developed that consists of a thin film evaporator for the direct solidification of the liquid waste, a pelletizer for producing hard pellets from the powdered wastes, a pellet storage unit, and a solidification unit for the final disposal. A pilot plant with waste treatment capacity of 200 kg/h was built in 1976 and has proved the system feasibility. This paper reports on pilot plant tests of the thin film evaporator and other components, tests on pellet deterioration during long term storage, and integrity tests on the final disposal of the pellet bitumen package.

1981-02-26

387

New York City Transit Hybrid and CNG Transit Buses: Final Evaluation Results  

Energy Technology Data Exchange (ETDEWEB)

This report describes the evaluation results for new Orion VII buses at NYCT with CNG propulsion and new hybrid propulsion.

2006-11-01

388

Microsoft Word - PressRelease Marine fuel 150711final21.doc  

Wastenet

be extremely limited or even negative due to higher maritime transport costs leading to a modal shift back to

389

MEMORANDUM OF AGREEMENT BETWEEN THE DEPARTMENT ...  

Science.gov (United States)

new technologies being evaluated by the ADTT. (d) Prepare the ... (g) Have final approval of the individual nominated for the position of Deputy. ADTT. (4) The ...

390

MEDICAL FINAL REVIEW MEMORANDUM OF ORIGINAL BLA  

Science.gov (United States)

... The AAT Z mutation involves a single amino acid substitution (glutamine for lysine) at position 342, resulting in abnormal folding and polymerization of the ...

391

Impact of associated injuries in the Floating knee: A retrospective study  

UK PubMed Central (United Kingdom)

BackgroundFloating knee injuries are usually associated with other significant injuries. Do these injuries have implications on the management of the floating knee and the final...Full Text Available

392

Human-modified ecosystems and future evolution  

UK PubMed Central (United Kingdom)

Our global impact is finally receiving the scientific attention it deserves. The outcome will largely determine the future course of evolution. Human-modified ecosystems are shaped by our activities...Full Text Available

2001-05-08

394

Geothermal materials program: strategy. Final report  

Energy Technology Data Exchange (ETDEWEB)

The following topics are discussed: program goal and objectives, program organization, and program status. Current program projects are described. (MHR)

1980-10-01

395

Gene Networks and the Neuroendocrine Regulation of Puberty  

UK PubMed Central (United Kingdom)

A sustained increase in pulsatile release of gonadotrophin releasing hormone (GnRH) from the hypothalamus is an essential, final event that defines the initiation of mammalian puberty. This...Full Text Available

2010-08-05

397

Final Report of OT-45, PVM-8, and RVTO Weather ...  

Science.gov (United States)

... A second hydrometeor sampler (MRI Model 1220 ... The cloud particle probe normally installed in ... to the northeast showed no significant movement. ...

1975-07-01

398

Final Environmental Baseline Survey Report For The Plum Brook ...  

Science.gov (United States)

9-foot 9-inch blocks of concrete with asphaltic-filled expansion joints. The concrete was placed on 4 to 6 inches of gravel or #4 stone. ...

399

FINAL REPORT CONTINUOUS SCANNING METEOROLOGICAL CAMERA SYSTEM FOR ...  

Science.gov (United States)

was deemed adequate and a cooling system designed utilizing the Melcor CP2-15- 10B thermoelectric module. Wall temperature achieved with this system was - 36 ...

400

Evaluation of the Cask Transportation Facility Modifications (CTFM) compliance to DOE order 6430.1A; FINAL  

International Nuclear Information System (INIS)

This report was prepared to evaluate the compliance of Cask Transportation Facility Modifications (CTFM) to DOE Order 6430.1A.

401

Evaluation of Upland Disposal of John F. Baldwin Ship ...  

Science.gov (United States)

... Accession Number : ADA270425. Title : Evaluation of Upland Disposal of John F. Baldwin Ship Channel Sediment. Descriptive Note : Final rept.,. ...

1993-07-01

402

Environmental Impact Analysis Process. Final Environmental ...  

Science.gov (United States)

... Abstract : The United States Air Force (USAF) currently operates the Milstar satellite system to provide Military Satellite Communication Systems ...

2001-02-15

403

Environmental Assessment | Science & Technology | US EPA  

Science.gov (United States)

Report) A Screening Assessment of the Potential Impacts of Climate Change on Combined Sewer Overflow (CSO) Mitigation in the Great Lakes and New England Regions (Final Report)...

2011-09-30

405

DSN Radio Science ... - InterPlanetary Network (IPN) Progress Report  

Science.gov (United States)

tions, final noise-power spectral density measurements were made. These measurements of the noise-power spectral density were not the desired phase- noise ...

406

DCAA Contract Audit Manual. Volume 2, Chapters 12 - 15 ...  

Science.gov (United States)

... Final accounting for extended to include ?mounts applicable ... is verified by the finance or disbursing ... the contractor must claim DLA, contractors must ...

1995-01-01

407

Cold vacuum drying facility design requirements; FINAL  

International Nuclear Information System (INIS)

This document provides the detailed design requirements for the Spent Nuclear Fuel Project Cold Vacuum Drying Facility. Process, safety, and quality assurance requirements and interfaces are specified.

408

Annual inside final - NASA  

Science.gov (United States)

The attendees this year included partic- ipants from space agencies, industry, and academia from Canada, the Czech Republic, France, Germany, Italy, Japan, ...

409

A Research Needs Assessment for waste plastics recycling: Volume 2, Project report. Final report  

Energy Technology Data Exchange (ETDEWEB)

This second volume contains detailed information on a number of specific topics relevant to the recovery/recycling of plastics.

1994-12-01

410

Hydration of swelling clay and bacteria interaction. An experimental in situ reaction study; Hydratation des argiles gonflantes et influence des bacteries. Etude experimentale de reaction in situ  

Energy Technology Data Exchange (ETDEWEB)

This study reports on the physical-chemical behaviour of swelling di-octahedral clays (smectites) and their interaction with aqueous solutions and bacteria (Shewanella putrefaciens). Experimental results are presented for compacted clays, hydrated under confined volume conditions, using a new type of reaction-cell (the 'wet-cell' of Warr and Hoffman, 2004) that was designed for in situ X-ray diffraction (XRD) measurement. For comparison, dispersed clay systems were studied using standard batch solutions subjected to varying degrees of agitation. The combination of time-dependent in situ XRD measurements with gravimetric measurements and calculated diffraction patterns using the CALCMIX software (Plancon and Drits, 1999) allowed to successful quantification of the dynamics of water uptake and storage. This analytical procedure combined with published water vapour adsorption data enabled determination of the abundance of structured water layers, ...

2008-01-15

411

Study on dynamic compaction of a buffer material mixed with bentonite for H.L.W. final disposal  

International Nuclear Information System (INIS)

In-situ compaction method is an alternative technology for the construction of a buffer at HLW final disposal. Understanding of the compaction characteristics of the proposed material is important for this method of construction. In this report, paying attention to the relation between compaction energy and the achieved maximum dry density, dynamic compaction characteristic of bentonite and silica sand mixture material is examined. (author)

2004-06-01

412

Photon final states at the Tevatron  

Energy Technology Data Exchange (ETDEWEB)

The authors present here several recent measurements involving associate production of photons and jets at the Tevatron. In particular, inclusive photon + met from D0, and photon + b-jets and photon + b-jet + leptons + MET from CDF are described in some detail. These measurements offer a good test of QCD predictions in rather complex final states.

2008-04-01

413

PFB coal fired combined cycle development program. Final report  

Energy Technology Data Exchange (ETDEWEB)

A final report is presented on the design, environmental characteristics, performance and maintainability, and economic analysis of a commercial 663 MWe coal-fired combined cycle power plant using pressurized fluidized bed combustion, and advanced technology in the hot gas cleanup system and gas turbines. Experimental data on the performance of each of the major components are included. (LCL)

1981-03-01

414

New physics effects on Higgs production at #gamma##gamma# colliders  

International Nuclear Information System (INIS)

We study heavy physics effects on the Higgs production in #gamma##gamma# fusion using the effective Lagrangian approach. We find that the effects coming from new physics may enhance the standard model predictions for the number of events expected in the final states b-barb, WW, and ZZ up to one order of magnitude, whereas the corresponding number of events for the final state t-bart may be enhanced up to two orders of magnitude.

1996-02-20

415

Natural radioactive materials occurrence in oil facilities  

International Nuclear Information System (INIS)

This work presents and describes the natural radioactive occurrence in oil facilities. The origins of the detected radioactivity and the reasons of its settlement are discussed. The measurements results carried out in oil facilities in the Neuquina basin (Argentine) are shown in this work. The aim is to describe the actual situation and to evaluate the magnitude of the problem. Finally, recommendations are proposed to protect the health of the workers, especially of those who carry out the cleaning of the equipment, and to guarantee an appropriate final disposal for the generated radioactive residuals. (author)

2001-06-01

416

High energy physics  

International Nuclear Information System (INIS)

The research of the CELLO collaboration is reviewed. After a description of the CELLO detector tests of QCD and hadronic final states by determination of the strong coupling constant and studies of inclusive production of electrons and muons in multihadronic events in e"+e"- annihilation are described. Then studies of deep inelastic e#gamma# scattering with lepton pair production and the study of the structure function of the photon are briefly described. Finally studies on QED processes are described together with tau decays. (HSI).

1983-11-01

417

Analysis of a 1200 kV circuit breaker for a gas insulated substation. Final report  

Energy Technology Data Exchange (ETDEWEB)

This report describes the work carried out to analyze and design a circuit for use in 1200 kV gas insulated substations. The first part of the project was devoted to a thorough analysis of the requirements for the circuit breaker from the standpoint of the electrical system in which it would operate. A conceptual design was selected and all of the components of the circuit breaker were designed, modeled and verified. Finally a plan was prepared for the construction of a complete circuit breaker.

1985-01-01

418

[Molecular cloning and expression of an isotoxin gene, alpha-bungarotoxin, from Bungarus multicinctus].  

Science.gov (United States)

Abstract: Snake venom contains a number of small proteins,enzymes and other components,which displays a broad spectrum of biological activities. With the ability of specifically binding on acetylcholine acceptor, alpha-bungarotoxins are not only useful molecular probes in investigating the mechanism of neural signal transmission, but also potential pharmic preparations for neural disease treatment. In current research,cDNAs of Bungarus multicinutus venom gland were synthesized using SMART cDNA amplification kit and then, alpha-bungarotoxin genes were cloned and sequenced. Total of 20 clones were sequenced representing 14 isotoxin mRNAs of alpha-bungarotoxins. Among those clones, a novel isotoxin gene was subcloned into two expression plasmids, alpha-BgTX/pQE30a and alpha-BgTX/pGEX-4T-1, and transformed into E. coli. After inducing with IPTG, fused protein of GST-alpha-BgTX was successfully expressed at level of 30% gross proteins of bacteria. More than 25% of fused ...

2005-07-01

419

[Fe]-hydrogenases in green algae: photo-fermentation and hydrogen evolution under sulfur deprivation  

Energy Technology Data Exchange (ETDEWEB)

Recent studies indicate that [Fe]-hydrogenases and H{sub 2} metabolism are widely distributed among green algae. The enzymes are simple structured and catalyze H{sub 2} evolution with similar rates than the more complex [Fe]-hydrogenases from bacteria. Different green algal species developed diverse strategies to survive under sulfur deprivation. Chlamydomonas reinhardtii evolves large quantities of hydrogen gas in the absence of sulfur. In a sealed culture of C. reinhardtii, the photosynthetic O{sub 2} evolution rate drops below the rate of respiratory O{sub 2} consumption due to a reversible inhibition of photosystem II, thus leading to an intracellular anaerobiosis. The algal cells survive under these anaerobic conditions by switching their metabolism to a kind of photo-fermentation. Although possessing a functional [Fe]-hydrogenase gene, the cells of Scenedesmus obliquus produce no significant amounts of H{sub 2} under S-depleted conditions. Biochemical ...

2002-12-01

420

Treatment of waste rock piles at Wood Cadillac using a reducing trickling filter; Traitement des resurgences du parc a residus miniers Wood Cadillac au moyen d'un biofiltre reducteur  

Energy Technology Data Exchange (ETDEWEB)

The Ministere des Ressources naturelles du Quebec (Quebec Natural Resources Department) implemented a remedial program to treat the waste rock pile at Wood Cadillac, located near Cadillac, in Abitibi, Quebec. The solutions proposed by INRS-Georessources and ENVIROCONSEIL are aimed at eliminating erosion and stabilizing the geochemical processes. Part of the proposed solution also included the use of a reducing trickling filter for the treatment of acid leachates, which also contain arsenic when exiting the waste rock pile. The trickling filter is composed of forest tailings in which the bacteria reduce the sulphates and stimulate the precipitation of arsenic into arsenic sulphates. This is a new treatment that was developed following laboratory experiments which demonstrated the feasibility of the process and quantified the parameters. A demonstration unit was constructed by ENVIROCONSEIL and installed at the Wood Cadillac waste rock pile under the supervision of ...

2000-07-01

421

Toxicological benchmarks for screening potential contaminants of concern for effects on soil and litter invertebrates and heterotrophic process  

International Nuclear Information System (INIS)

One of the initial stages in ecological risk assessments for hazardous waste sites is the screening of contaminants to determine which of them are worthy of further consideration as open-quotes contaminants of potential concern.close quotes This process is termed open-quotes contaminant screening.close quotes It is performed by comparing measured ambient concentrations of chemicals to benchmark concentrations. Currently, no standard benchmark concentrations exist for assessing contaminants in soil with respect to their toxicity to soil- and litter-dwelling invertebrates, including earthworms, other micro- and macroinvertebrates, or heterotrophic bacteria and fungi. This report presents a standard method for deriving benchmarks for this purpose, sets of data concerning effects of chemicals in soil on invertebrates and soil microbial processes, and benchmarks for chemicals potentially associated with United States Department of Energy sites. In addition, literature ...

422

The multidrug resistance efflux complex, EmrAB from Escherichia coli forms a dimer in vitro  

International Nuclear Information System (INIS)

Tripartite efflux systems are responsible for the export of toxins across both the inner and outer membranes of Gram negative bacteria. Previous work has indicated that EmrAB-TolC from Escherichia coli is such a tripartite system, comprised of EmrB an MFS transporter, EmrA, a membrane fusion protein and TolC, an outer membrane channel. The whole complex is predicted to form a continuous channel allowing direct export from the cytoplasm to the exterior of the cell. Little is known, however, about the interactions between the individual components of this system. Reconstitution of EmrA + EmrB resulted in co-elution of the two proteins from a gel filtration column indicating formation of the EmrAB complex. Electron microscopic single particle analysis of the reconstituted EmrAB complex revealed the presence of particles approximately 240 x 140 A, likely to correspond to two EmrAB dimers in a back-to-back arrangement, suggesting the dimeric EmrAB form is the ...

2009-03-06

423

Sulfur and carbon cycling in a flue gas desulfurization sludge disposal site.  

Science.gov (United States)

Products of a power plant flue gas desulfurization scrubber are discharged into a pond as sludge consisting of calcite (initial delta13C 3.2-3.8 per thousand), gypsum (initial delta34S 7.6-8.6 per thousand), and aqueous solution. Reducing conditions exist below a boundary that appears to move vertically as a function of changes in pond water level. Under reducing conditions, bacteria partially reduce aqueous sulfate to low-delta34S sulfide, consuming organic carbon and generating low-delta13C bicarbonate. Under oxidizing conditions, sulfide is converted to sulfate, leading to calcite dissolution, gypsum precipitation, and isotopic re-equilibration of remaining calcite with dissolved bicarbonate near the pond surface. The gypsum has delta34S near 6 per thousand, and calcite has delta13C as low as -1.7 per thousand; the changes from initial values correspond to predictions based on isotopic balance and reaction stoichiometry. The pond largely contains the products of ...

2003-04-01

424

Study on biosorption kinetics and thermodynamics of uranium by Citrobacter freudii  

Energy Technology Data Exchange (ETDEWEB)

Biosorption has been developed as an effective and economic method to treat wastewater containing low concentrations of metal pollutants. In this study, a bacterium, Citrobacter freudii, was used as a biosorbent to adsorb uranium ions. The thermodynamics and kinetics of this adsorption, as well as its mechanism, were investigated. The results indicated that the biosorption rate could be better described by a pseudo 2nd-order model than a pseudo 1st-order model. The adsorption of U (VI) proceeded very rapidly in the first 30 min and subsequently slowed down continuously for a long period. The biosorption isotherm of uranium by C. freudii could be described well by the Langmuir or Freundlich isotherm, and the latter was better. The thermodynamics parameters, {delta}H{sup o}, {delta}G{sup o}, and {delta}S{sup o} were calculated according to the results of the experiment, which showed this biosorption as being endothermic and spontaneous. The authors investigated the active sites of ...

2008-01-15

425

Role of the Yersinia pestis Ail Protein in Preventing a Protective Polymorphonuclear Leukocyte Response During Bubonic Plague.  

Science.gov (United States)

The ability of Yersinia pestis to forestall the mammalian innate immune response is a fundamental aspect of plague pathogenesis. In this study, we examined the effect of Ail, a 17-kDa outer membrane protein that protects Y. pestis against complement-mediated lysis, on bubonic plague pathogenesis in mice and rats. The Y. pestis ail mutant was attenuated for virulence in both rodent models. The attenuation was greater in rats than in mice, which correlates with the ability of normal rat serum, but not mouse serum, to kill ail-negative Y. pestis in vitro. Intradermal infection with the ail mutant resulted in an atypical, subacute form of bubonic plague associated with extensive recruitment of polymorphonuclear leukocytes (PMN or neutrophils) to the site of infection in the draining lymph node and the formation of large purulent abscesses that contained the bacteria. Systemic spread and mortality were greatly attenuated, however, and a productive adaptive immune ...

2011-10-01

426

Report on a survey in fiscal 1999. Survey on industrial utilization of microorganism reaction mechanisms under anaerobic condition; 1999 nendo kenki jokenka ni okeru biseibutsu hanno kiko no kogyoteki riyo ni kansuru chosa hokokusho  

Energy Technology Data Exchange (ETDEWEB)

Industrial utilization of reaction mechanisms of microorganisms under anaerobic condition permits structuring energy saving type production processes. The present survey has investigated features of new microorganisms under anaerobic condition and the status of researches thereon inside and outside the country, and discussed their future applications. Chapter 1 compares anaerobic microorganisms and functions of microorganism under anaerobic condition with those aerobic to describe their general features, and describes the purpose of this survey and the summary of the investigations. Chapter 2 surveys the current status of technologies to utilize microorganisms under anaerobic condition. Chapter 3 outlines metabolic characteristics of the anaerobic microorganisms, and extracts functions effective for material production by different anaerobic microorganisms to describe their applicability. Chapter 4 evaluates the system classification for the anaerobic microorganisms utilizing the basic ...

2000-03-01

427

Quantification of antibiotic drug potency by a two-compartment radioassay of bacterial growth  

Energy Technology Data Exchange (ETDEWEB)

The two-compartment radioassay for microbial kinetics based on continuous measurement of the {sup 14}CO{sub 2} released by bacterial metabolism of 14C-labeled substrate offers a valuable approach to testing the potency of antimicrobial drugs. By using a previously validated radioassay with gram-positive and gram-negative bacteria, a group of protein synthesis inhibitors was evaluated for their effect on microbial growth kinetics. All tested drugs induced changes in both the slopes and intercepts of the growth curves. An exponential growth model was applied to quantify the drug effect on the processes of bacterial {sup 14}CO{sub 2} liberation and cell generation. The response was measured in terms of a generation rate constant. A linear dependence of the generation rate constant on the dose of spectinomycin was observed with Escherichia coli. Sigmoidal-shaped curves were found in the assays of chloramphenicol and tetracycline. The implications of dose-response ...

1990-06-01

428

Methanogens and sulfate-reducing bacteria in oil sands fine tailings waste.  

Science.gov (United States)

In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of methane in the fine tailings zone of this and other settling basins may affect the performance of these settling basins and impact reclamation options. Enumeration studies found methanogens (10(5)-10(6) MPN/g) within the fine tailings zone of various oil sands waste settling basins. SRB were also present (10(4)-10(5) MPN/g) with elevated numbers when sulfate was available. The methanogenic population was robust, and sample storage up to 9 months at 4 degrees C did not cause the MPN values to change. Nor was the ability of the consortium to produce methane delayed or less efficient after storage. Under laboratory conditions, fine tailings samples released 0.10-0.25 mL CH4 ...

2000-10-01

429

Intracellular monitoring of superoxide dismutase expression in an Escherichia coli fed-batch cultivation using on-line disruption with at-line surface plasmon resonance detection.  

Science.gov (United States)

An on-line cell disruption system for at-line monitoring of the intracellular concentration of recombinant human superoxide dismutase (rhSOD) in a genetically modified Escherichia coli strain, HMS174(DE3) (pET11a/rhSOD), in bioreactor cultivations is described. The sampled bacteria were disrupted on-line by rapid mixing with a nonionic detergent. The recombinant protein content of the lysed bacterial sample was quantitated by a subsequent surface plasmon resonance biosensor with a specific monoclonal antibody. Extraction efficiency of the monitoring system was optimized with respect to the flow rate ratio of the cell suspension and the detergent at relevant cell densities with the aim to attain rapid monitoring. Monitoring was demonstrated for a shake flask culture and a glucose-limited fed-batch cultivation. The results are compared with a traditional enzyme-linked immunosorbent assay method showing a correlation coefficient of R2 = 0.97. Extraction efficiency of ...

2005-07-01

430

Increase of unsaturated fatty acids under high pressure in a deep-sea bacterium; Shinkai kara bunrisareta taiatsusei biseibutsu DSS12 kabu no baiyo ondo, baiyo atsuryoku to kintai shibosan sosei no henka  

Energy Technology Data Exchange (ETDEWEB)

A cellular membrane exchanges substances into and from the cell and protects the cell interior by maintaining semi-fluidity rather than be being solid. It is known that microorganisms maintain the fluidity in correspondence with varying environmental temperatures by changing and adjusting the composition of fatty acids which constitute the cellular membrane lipids. As part of the studies to elucidate the pressure withstanding mechanism in deep-sea bacteria, this paper investigates what variations the fatty acid composition, which constitutes cellular membranes of the DSS12 strain, a pressure withstanding bacterium growing well under either normal pressure or high pressures, will show under different culturing temperatures and pressures. Culture under low temperatures and culture under high pressures increase content of unsaturated fatty acids including icosapentanoic acid. Culture under high temperatures or normal pressure increases content of such unsaturated ...

1997-03-01

431

Impact of climate change on carbon cycle in freshwater ecosystems  

Energy Technology Data Exchange (ETDEWEB)

The impacts of the expected climate change on Finnish lake ecosystems were studied with the biota of the mesohumic Lake Paeaejaervi, southern Finland. Experimental conditions, from small-scale experiments on single species level to a large-scale ecosystem manipulation, were established to simulate directly the future climate and/or loading of nutrients and dissolved organic matter (DOM) from the drainage area. The experimental studies were accomplished by modelling the carbon flow in the pelagic food web as well as the growth of littoral macrophytes. The main hypothese tested were as follows: As a consequence of the climate change (rising temperature and increasing precipitation) the loading of nutrients and dissolved organic matter (DOM) from the drainage area to the lake will increase. In the pelagic zone this will be first reflected i higher productivity of primary producers and bacteria, but will later affect the entire food chain. Increase in atmospheric ...

1996-12-31

432

Identification of sucrose binding, membrane proteins using a photolyzable sucrose analog. [P. saccharophila  

Energy Technology Data Exchange (ETDEWEB)

The sucrose derivative 6'-deoxy-6'-(2-hydroxy-4-azido)benzamidosucrose (6'-HABS) was prepared from sucrose (via 6'-deoxy-6'-aminosucrose) and 4-amino-salicylic acid. 6'-HABS is a competitive inhibitor of sucrose influx into protoplasts from developing soybean cotyledons and of sucrose binding to membranes from the bacteria P. saccharophila. The Ki for inhibition in the soybean protoplasts was 75..mu..M. 6'-Deoxy-6'-(2-hydroxy-3-/sup 125/Iodo-4-azido)benzamidosucrose was prepared by lactoperoxidase iodination of 6'-HABS. Upon photolysis in the presence of membranes from P saccharophila, label from the photoprobe is incorporated into a sucrose inducible polypeptide of mass 84 KD in SDS-PAGE. The polypeptide is protected from labeling by the inclusion of sucrose in the photolysis mixture. Photolysis conditions which lead to specific labeling of the sucrose protectable polypeptide in ...

1986-04-01

433

IAEA RESEARCH CONTRACTS SECOND ANNUAL REPORT. Technical Reports Series No. 9  

Science.gov (United States)

Summaries are presented for those research contracts which expired between December 31, 1960 and December 31, 1981. Topics covered include: factors controlling distribution of fission products in biosphere, non-destructive methods of evaluating the U/sup 235/ and Pu content of irradiated fuel elements, studies of contamination in local marine resources, tracer studies of anemia, non-destructive analysis of irradiated fuel elements using a flux integrating monitor, mechanism of proteolysis of I/sup 131/-labeled fibrinogen, radiosensitivity of spermatogonia of Drosophila melel elements by gamma scanning, uptake and loss of radioactive material by marine bacteria, factors which influence the movement of radioactive strontium from soils to plants, biological concentration of fission products in mollusks from water, decay scheme of Tl/sup 210/, calcium balance in metabolic bone disease, development of radioactive drugs with special reference ...

1962-01-01

434

Geochemical study on origin of natural gases in Japanese oil and gas fields  

Energy Technology Data Exchange (ETDEWEB)

The origin of natural gas in the Japanese oil and gas fields are geochemically studied. Samples are taken from structural natural gas, surface gas seepage, water-dissolved natural gas and coal-field gas of the Japan Sea coast area. The origins of primary hydrocarbons are classified into bacteria gas and thermogenic gas, the latter being subclassified into gas in oil production zone with the per million deviation of the carbon isotope (/sup 13/C/sup 1/) of methane less than -35 and those gases with maturity higher than this. Surface gas seepage is subjected to migration and bacterial oxidation. Coal gas is similar to oil gas. The CO/sub 2/ concentration is 0 - 2% and /sup 13/CO/sub 2/ deviations from -30 - +30. The origin can be barely estimated from the isotopic composition because of the wide secondary change in the composition. The origin and migration of gas from the gas field of northern Niigata are not similar to those in the southern area, which consist of a ...

1988-05-01

435

Genetic engineering of group 2 sigma factor SigE widely activates expressions of sugar catabolic genes in Synechocystis species PCC 6803.  

Science.gov (United States)

Metabolic engineering of photosynthetic organisms is required for utilization of light energy and for reducing carbon emissions.Control of transcriptional regulators is a powerful approach for changing cellular dynamics, because a set of genes is concomitantly regulated. Here, we show that overexpression of a group 2 ? factor, SigE, enhances the expressions of sugar catabolic genes in the unicellular cyanobacterium, Synechocystis sp. PCC 6803. Transcriptome analysis revealed that genes for the oxidative pentose phosphate pathway and glycogen catabolism are induced by overproduction of SigE. Immunoblotting showed that protein levels of sugar catabolic enzymes, such as glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glycogen phosphorylase, and isoamylase, are increased. Glycogen levels are reduced in the SigE-overexpressing strain grown under light. Metabolome analysis revealed that metabolite levels of the TCA cycle and acetyl-CoA are significantly altered by SigE ...

2011-07-11

436

Gene expression analysis after low dose ionising radiation exposure of the developing organism  

International Nuclear Information System (INIS)

Measuring gene expression using microarrays is relevant to many areas of biology and medicine, such as follow up of developmental stages and diseases onset, and treatment study. Since there can be tens of thousands of distinct probes on an array, each micro array experiment can accomplish the equivalent number of genetic tests in parallel. Arrays have therefore dramatically accelerated many types of investigations. For example, microarrays can be used to identify stress response genes by comparing gene expression in challenged versus normal cells. In the Molecular and Cellular Biology lab (MCB), the micro array experiments are performed within the Genomic Platform, fully equipped to analyse either the behaviour of bacteria during long space flight, the effect of low dose ionising radiation on the developing organism in mice, or the human individual radiation sensitivity. For the low dose effect, two main stages of development are of interest; 1) the gastrula stage ...

2007-09-01

437

Formation of colloidal silver nanoparticles stabilized by Na+-poly(gamma-glutamic acid)-silver nitrate complex via chemical reduction process.  

Science.gov (United States)

Macromolecular and polyanionic Na(+)-poly(gamma-glutamic acid) (PGA) silver nitrate complex acted as both a metal ion provider and a particle protector to fabricate nanosized silver colloids under chemical reduction by dextrose. The formation and size of particles have been characterized from transmission electron microscopy (TEM), dynamic light scattering analysis and UV-vis spectrophotometer. The results showed that the average particle size was 17.2+/-3.4 to 37.3+/-5.5 nm, apparently depending on the complex concentration. It was found that the rate constant and conversion of silver nanoparticles were proportional to the concentration of PGA. The growth mechanism of nanosized silver colloid was fully discussed. In addition, the in vitro cytotoxicity evaluated by L929 fibroblasts proliferation and antibacterial activity against Gram-positive strain (methicillin-resistant S. aureus (MRSA)) and Gram-negative strain (P. aeruginosa) bacteria have been assessed. ...

2007-05-18

438

Evolution of a molecular switch: universal bacterial GTPases regulate ribosome function.  

Science.gov (United States)

The GTPases comprise a protein superfamily of highly conserved molecular switches adapted to many diverse functions. These proteins are found in all domains of life and often perform essential roles in fundamental cellular processes. Analysis of data from genome sequencing projects demonstrates that bacteria possess a core of 11 universally conserved GTPases (elongation factor G and Tu, initiation factor 2, LepA, Era, Obg, ThdF/TrmE, Ffh, FtsY, EngA and YchF). Investigations aimed at understanding the function of GTPases indicate that a second conserved feature of these proteins is that they elicit their function through interaction with RNA and/or ribosomes. An emerging concept suggests that the 11 universal GTPases are either necessary for ribosome function or transmitting information from the ribosome to downstream targets for the purpose of generating specific cellular responses. Furthermore, it is suggested that progenitor GTPases were early regulators of RNA ...

2001-07-01

439

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay  

Energy Technology Data Exchange (ETDEWEB)

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis lucida, hepatoma-derived cell cultures exposed to cadmium or copper. Three proteins ...

1994-08-01

440

Evaluation of chicken manure, kenaf, and phanerochaete chrysosporium (white rot fungus) as enhancers of polychlorinated biphenyl biodegradation  

Energy Technology Data Exchange (ETDEWEB)

In this 150-day study, chicken manure, kenaf, and white rot fungus were added to soil microcosms in an attempt to enhance the degradation of polychlorinated biphenyls. The soil was contaminated with commercial PCB mixtures. Dishes were ammended with 5% dry weight chicken manure, 1% dry weight kenaf, and 1% dry weight kenaf plus Phanerochaete chrysosporium inoculant. PCB concentrations were determined at 30 day intervals by soxhlet extraction and gas chromatography analyses. Preliminary results of microbial populations and PCB degradation are presented. At 90 days, the microcosms amended with chicken manure had significantly higher populations of bacteria, fungi, and actinomycetes. However, at 120 days, these soils underwent great reductions in actinomycete and bacterial populations. Through 60 days, the concentration of the PCBs Aroclor 1242 and 1248 had its greatest reduction in the kenaf amended soils. The concentration of Aroclor 1260 either increased or stayed ...

1995-12-31

441

Enzymatic mechanism in low chloride media: influence of glucose oxidase on the electrochemical behaviour of AISI 316L stainless steel; Mecanisme enzymatique en milieux faiblement chlorures: influence de la glucose oxydase sur le comportement electrochimique de l'acier inoxydable AISI 316L  

Energy Technology Data Exchange (ETDEWEB)

The open circuit potential (OCP) of stainless steels immersed in natural waters generally increases with time. This phenomenon is strongly linked to the formation of a bio-film on the surface. Several studies have proved that the bio-film modifies the reaction of reduction of dissolved oxygen and that it acts mainly on the cathodic processes. One of the hypotheses explaining the action of the bio-film involves certain bacteria which use dissolved oxygen and extracellular enzymes to produce reactive oxygen species. Among this kind of metabolites is hydrogen peroxide H{sub 2}O{sub 2}. This compound interacts with the passive layer of stainless steels and affects their electrochemical behaviour, even when the chloride concentration is low. The aim of this work is to study the influence of an enzymatic reaction (of the oxidoreductase type) on anodic and cathodic processes on AISI 316L stainless steel. Experiments are carried out in two artificial electrolytes ...

2006-07-01

442

Engineering of a psychrophilic bacterium for the bioremediation of aromatic compounds  

Science.gov (United States)

Microbial degradation of aromatic hydrocarbons has been studied with the aim of developing applications for the removal of toxic compounds. Efforts have been directed toward the genetic manipulation of mesophilic bacteria to improve their ability to degrade pollutants, even though many pollution problems occur in sea waters and in effluents of industrial processes which are characterized by low temperatures. From these considerations the idea of engineering a psychrophilic microorganism for the oxidation of aromatic compounds was developed.In a previous paper it was demonstrated that the recombinant Antarctic Pseudoalteromonas haloplanktis TAC125 (PhTAC/tou) expressing a toluene-o-xylene monooxygenase (ToMO) is able to convert several aromatic compounds into corresponding catechols. In our work we improved the metabolic capability of PhTAC/tou cells by combining action of recombinant ToMO enzyme with that of the endogenous P. haloplanktis TAC125 laccase-like ...

2010-01-27

443

Does adenine incorporation into nucleic acids measure total microbial production:: a response to comments by Fuhrman et al  

Science.gov (United States)

The uptake and incorporation of (/sup 3/H)adenine as a measure of total microbial (bacteria and unicellular algae) nucleic acid synthesis depends on the validity of several assumptions. A unique characteristic of the (/sup 3/H)adenine method is the ability to measure the specific radioactivity (nCi pmol/sup -1/) of the immediate precursor pool of the adenine incorporated into nucleic acids. This measurement permits correction for isotope dilution when the added radiotracer mixes with exogenous pools of structurally related compounds before uptake and with endogenous pools after transport into the cells. The immediate precursors to (/sup 3/H)adenine incorporation into cellular RNA and DNA are ATP and dATP. Since the intracellular dATP pool is in isotopic equilibrium with the ATP pool, one can determine the specific radioactivity of both precursor pools by measuring that of the intracellular ATP pool. If the specific activity of the precursor pool is not measured, ...

1986-11-01

444

Control of microbially generated hydrogen sulfide in produced waters  

Energy Technology Data Exchange (ETDEWEB)

Production of hydrogen sulfide in produced waters due to the activity of sulfate-reducing bacteria (SRB) is a potentially serious problem. The hydrogen sulfide is not only a safety and environmental concern, it also contributes to corrosion, solids formation, a reduction in produced oil and gas values, and limitations on water discharge. Waters produced from seawater-flooded reservoirs typically contain all of the nutrients required to support SRB metabolism. Surface processing facilities provide a favorable environment in which SRB flourish, converting water-borne nutrients into biomass and H{sub 2}S. This paper will present results from a field trial in which a new technology for the biochemical control of SRB metabolism was successfully applied. A slip stream of water downstream of separators on a produced water handling facility was routed through a bioreactor in a side-steam device where microbial growth was allowed to develop fully. This slip stream was then ...

1995-12-31

445

Containment of genetically engineered organisms after application to subsurface environments. Technical completion report  

Energy Technology Data Exchange (ETDEWEB)

The feasibility of containing genetically engineered bacteria with enhanced dehalogenating properties for in situ bioremediation was investigated. (1) An agarose matrix microbead protocol and a detection system for contained microorganisms or DNA were developed. Multiplex Polymerase Chain Reaction (PCR) allowed tracking of a consortium of encapsulated organisms or several gene targets from a single species. Gene sequences encoding the enzymes responsible for initiating the biodegradation of toluene, octane, and 2,4-D were detected by multiplex PCR and nucleic acid probes from similar to 1-10 biodegradative cells/g soil. Improved DNA extraction methods resulted in PCR reactions detecting similar to 6 cells/g soil. (2) The pcpB gene (for the broad-spectrum detoxicant pentachlorophenol (PCP) hydroxylase) isolated from Flavobacterium sp. strain ATCC 39723 was used in attempts to develop an improved dehalogenating recombinant microorganism for containment experiments.

1993-06-01

446

Construction and evaluation of a double mutant of Shigella flexneri as a candidate for oral vaccination against shigellosis.  

Science.gov (United States)

Based on studies on the genetic and molecular basis of Shigella flexneri invasive properties, we have constructed and evaluated a double mutant of S. flexneri serotype 5 for utilization as a live attenuated oral vaccine against shigellosis. The first mutation, icsA, blocks intracellular spread of bacteria as well as cell-to-cell infection. It affects the capacity of the invasive pathogen to form large abscesses in epithelia. The second mutation, iuc, eliminates production of the siderophore aerobactin thus impairing growth of the bacterium within tissues. This double mutant, SC5700 appeared safe when administered intragastrically to macaque monkeys as three doses (5 x 10(10) c.f.u. each) at weekly intervals. Protection against a challenge by the wild type isolate (M90T) was observed 4 weeks after the last vaccine inoculation. Duration of carriage was considerably reduced as compared to the control group in which all animals had developed severe dysentery. ...

1989-10-01

447

Complete genome sequence of Conexibacter woesei type strain (ID131577T)  

Energy Technology Data Exchange (ETDEWEB)

The genus Conexibacter (Monciardini et al. 2003) represents the type genus of the family Conexibacteraceae (Stackebrandt 2005, emend. Zhi et al. 2009) with Conexibacter woesei as the type species of the genus. C. woesei is a representative of a deep evolutionary line of des-cent within the class Actinobacteria. Strain ID131577T was originally isolated from temperate forest soil in Gerenzano (Italy). Cells are small, short rods that are motile by peritrichous fla-gella. They may form aggregates after a longer period of growth and, then as a typical charac-teristic, an undulate structure is formed by self-aggregation of flagella with entangled bacteri-al cells. Here we describe the features of the organism, together with the complete sequence and annotation. The 6,359,369 bp long genome of C. woesei contains 5,950 protein-coding and 48 RNA genes and is part of the Genomic Encyclopedia of Bacteria and Archaea project.

2010-01-01

448

Complete genome sequence of Actinosynnema mirum type strain (101T)  

Energy Technology Data Exchange (ETDEWEB)

Actinosynnema mirum Hasegawa et al. 1978 is the type species of the genus, and is of phylogenetic interest because of its central phylogenetic location in the Actino-synnemataceae, a rapidly growing family within the actinobacterial suborder Pseudo-nocardineae. A. mirum is characterized by its motile spores borne on synnemata and as a producer of nocardicin antibiotics. It is capable of growing aerobically and under a moderate CO2 atmosphere. The strain is a Gram-positive, aerial and substrate mycelium producing bacterium, originally isolated from a grass blade collected from the Raritan River, New Jersey. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first complete genome sequence of a member of the family Actinosynnemataceae, and only the second sequence from the actinobacterial suborder Pseudonocardineae. The 8,248,144 bp long single replicon genome with its 7100 protein-coding and 77 RNA genes is part of the ...

2009-05-20

449

Competition by inhibitory oligonucleotides prevents binding of CpG to C-terminal TLR9  

British Library Electronic Table of Contents (United Kingdom)

Abstract TLR9 recognizes unmethylated CpG-containing DNA commonly found in bacteria. Synthetic oligonucleotides containing CpG-motifs (CpG ODNs) recapitulate the activation of TLR9 by microbial DNA, whereas inversion of the CG dinucleotide within the CpG motif to GC (GpC ODNs) renders such ODNs inactive. This difference cannot be attributed to binding of ODNs to the full-length TLR9 ectodomain, as both CpG and GpC ODNs bind comparably. Activation of murine TLR9 requires cleavage into an active C-terminal fragment, which binds CpG robustly. We therefore compared the ability of CpG and GpC ODNs to bind to full-length and C-terminal TLR9, and their impact on the cleavage of TLR9. We found that CpG binds better to C-terminal TLR9 when compared with GpC, despite comparably low binding of both O...

2011-01-01

450

Characteristics of volatile fatty acid decomposition in anaerobic fluidized bed  

Energy Technology Data Exchange (ETDEWEB)

The growth kinetics constants and concentration of active attached biomass in an anaerobic fluidized bed which decomposes acetic, propionic and butyric acid were estimated. The mixture of above mentioned fatty acids was supplied to the fluidized bed in the range of hydraulic retention time (HRT) from 0.25 to 2 days. After the effluent reached in a steady state in quality, batch experiments were conducted separately with each fatty acid as a substrate in order to investigate the decomposition characteristics of each substrate by attached biomass. In order to estimate the parameter values of the growth kinetics of the bacteria, batch experiments were also conducted under the completely mixed condition using detached biomass from a support material. The changes of fatty acid concentrations with time were clearly expressed with the Monod growth model. Maximum specific substrate decomposition rates and saturation constants, and active biomass concentrations were ...

1988-06-01

451

Anaerobic degradation of DCM diffusing through clay  

Energy Technology Data Exchange (ETDEWEB)

Two series of diffusion tests were performed to examine the degradation of dichloromethane (DCM) as it diffuses through clay. The first series showed the use of a synthetic leachate with no significant initial bacterial population diffusing through a plug of intact clay; there was an induction period of 95--135 d, during which diffusion was as expected in the absence of degradation, followed by a second stage, where degradation occurred with an apparent half-life of less than 55 d at a temperature of 24 C. The second series of tests examined the diffusion of an actual leachate from the Keele Valley Landfill (KVL) (which provided both nutrients and a source of bacteria), through a compacted clay. In these tests, the induction period was reduced to 40--60 d, after which the apparent half-life was 20 d or less at 27 C. The diffusion coefficient for DCM was approximately 8 {times} 10{sup {minus}10} m{sup 2}/s, with partitioning coefficient K{sub d} = 1.5 cm{sup 3}/g. ...

1997-12-01

452

Adaptation of oral streptococci to low pH.  

Science.gov (United States)

The strategies employed by oral streptococci to resist the inimical influences of acidification reflect the diverse and dynamic niches of the human mouth. All of the oral streptococci are capable of rapid degradation of sugar to acidic end-products. As a result, the pH value of their immediate environment can plummet to levels where glycolysis and growth cease. At this point, the approaches for survival in acid separate the organisms. Streptococcus mutans, for example, relies on its F-ATPase, to protect itself from acidification by pumping protons out of the cells. S. salivarius responds by degrading urea to ammonia and S. sanguis produces ammonia by arginolysis. The mechanisms by which these organisms regulate their particular escape route are now being explored experimentally. The picture that emerges is that the acid-adaptive regulatory mechanisms of the oral streptococci differ markedly from those employed by Gram-negative bacteria. What remains to be ...

2000-01-01

453

Activity of Citrus Essential Oils against-Escherichia coli-O157:H7 and-Salmonella-spp. and Effects on Beef Subprimal Cuts under Refrigeration  

British Library Electronic Table of Contents (United Kingdom)

Abstract:- Escherichia coli-O157:H7 and-Salmonella-spp. are bacterial pathogens often associated with beef, and cause many cases of foodborne illness each year in the United States. During beef slaughter and processing, these bacteria may spread from the hide or intestines to the carcass. The objective of this research was to investigate the use of naturally occurring compounds citrus essential oils (CEOs) extracted from orange peel to reduce or eliminate these pathogens at the chilling stage of processing, or during fabrication. Brisket flats (used to simulate beef subprimals) were spot inoculated with approximately 6 log of surrogate generic-E. coli-cocktail (previously shown to be identical in growth and survival parameters to-E. coli-O157:H7 and-Salmonella-spp.). Following drying, CEOs...

2011-01-01

454

Active packaging of ground beef patties by edible zein films incorporated with partially purified lysozyme and Na2EDTA  

British Library Electronic Table of Contents (United Kingdom)

Summary In this study, antimicrobial activity of zein films incorporated with partially purified lysozyme and disodium ethylenediaminetetraacetic acid (Na2EDTA) has been tested on selected pathogenic bacteria and refrigerated ground beef patties. The developed films containing 700-g-cm-2 lysozyme and 300-g-cm-2 Na2EDTA showed antimicrobial activity on Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium. The application of lysozyme and Na2EDTA incorporated zein films on beef patties significantly decreased total viable counts (TVC) and total coliform counts after 5-days of storage compared to those of control patties (P-2EDTA or Na2EDTA alone significantly slowed down the oxidative changes in patties during storage (P-P-2EDTA for active packaging of refrigerated mea...

2011-01-01

455

A fluorescent aminolipid from a green photosynthetic bacterium  

Energy Technology Data Exchange (ETDEWEB)

A preliminary chemical characterization is presented of phosphate-free aminolipids isolation from Chlorobium limicola f. thiosulfatophilum. The lipid contains no phosphorus, glycerol, sugar, ornithine, or lysine. Ultraviolet absorption and fluorescence spectra indicate that the amino moiety of the lipid is an aromatic heterocyclic compound. Infrared spectra indicate that the lipid is a secondary or tertiary amide, and gas chromatograhic analysis of the hydrolyzed lipid shows that for each 1100 g of lipid, 1 mol of myristic acid (C/sub 14:0/) is linked in an amide bond. Acid hydrolysis of the lipid yields two fluorescent substances, A (ninhydrine positive) and B (negative), in addition to myristic acid. Proton nuclear magnetic resonance (NMR) studies indicate that substance A contains a butyl groups attached to a conjugated ring carbon, two equivalent ethyl groups attached to one or two nitrogen atoms, and two downfield protons (8.4 ppm), perhaps attached to a ring carbon adjacent to a ...

1983-04-12

456

US Department of Energy Region IV Unconventional Gas Program: summary and analysis  

Energy Technology Data Exchange (ETDEWEB)

The DOE Region IV Unconventional Gas Program involved the evaluation of unconventional gas resources at ten sites in the coal fields of the Eastern US. These projects dealt mainly with coalbed methane resources, although three of them also examined potential gas resources in Devonian black shales. The resource evaluations were accomplished primarily through recovery of core samples of potential gas-bearing strata and determination of specific gas content using the US Bureau of Mines direct method. In some cases actual gas production from the test holes was evaluated. Four of the projects were sited in the Warrior Basin, three in the Central Appalachian Basin, and one each in the Northern Appalachian Basin, the Deep River Basin of North Carolina, and the Valley Coal Fields of Virginia. Results from three of the projects, two in the Warrior Basin and one in the Northern Appalachian Basin, indicated the potential for economic recovery of coalbed methane. The projects included in this ...

1984-12-01

457

The specific heat capacity and thermal conductivity of normal liquid "3He  

International Nuclear Information System (INIS)

By observing the diffusion of a heat pulse along a 10-cm column of normal liquid "3He with the aid of two vibrating wire thermometers, it has been possible to measure the heat capacity C and thermal conductivity K of the liquid in the temperature range from T /sub c/ to 10 mK and at pressures of 0.21, 4.39, 9.97, 20.01, and 29.32 bar. By using a Pt NMR thermometer, an LCMN thermometer, and a "3He melting curve thermometer calibrated using the melting curve given by Greywall in 1983, a temperature scale has been established and (1) it has been shown that this melting curve is consistent in the temperature range 5-22 mK with the Korringa law for the Pt thermometer with a Korringa constant of 29.8 +/- 0.2 sec mK, (2) departures have been observed from the Curie-Weiss law for LCMN at low temperatures, and (3) values of the superfluid transition temperature have been obtained that are about 4% lower than the Helsinki values. The measured heat capacities agree well with those of Greywall, ...

1986-01-01

458

The Chicago Handbook for Teachers: A Practical Guide to the College Classroom. Second Edition  

Science.gov (United States)

Those who teach college students have extensive training in their disciplines, but unlike their counterparts at the high school or elementary school level, they often have surprisingly little instruction in the craft of teaching itself. "The Chicago Handbook for Teachers, Second Edition", is an extraordinarily helpful guide for anyone facing the daunting challenge of putting together a course and delivering it successfully. Representing teachers at all stages of their careers, the authors, including distinguished historian Alan Brinkley, offer practical advice for almost any situation a new teacher might face, from preparing a syllabus to managing classroom dynamics. Beginning with a nuts and bolts plan for designing a course, the handbook also explains how to lead a discussion, evaluate your own teaching, give an effective lecture, supervise students' writing and research, create and grade exams, and more. This new edition is thoroughly revised for contemporary concerns, with updated ...

2011-06-01

459

Sea-to-sky airshed ambient quality monitoring report : 1984-2001  

Energy Technology Data Exchange (ETDEWEB)

This report provides information to government and industry to enable decision-making and management of local environmental quality conditions. The need for a summary air quality report has arisen in recent years due to growing concerns about air quality. Increased growth in population and the tourism industry is placing additional pressures on environmental quality. The report summarized meteorological and air quality data collected by the British Columbia Ministry of Water, Land and Air Protection in the Sea-to-Sky airshed from 1984 to 2001. The airshed is located in the Lower Mainland region of British Columbia. Air quality monitoring was conducted for various periods at 18 different stations and meteorological monitoring was conducted at 4 locations. A list of both provincial and national air quality objectives and standards was provided, with definitions and reference levels from the Canadian Environmental Protection Act. Meteorological data was presented on a station by station ...

2004-09-01

460

Results of oil-shale investigations in northeastern Nevada  

Energy Technology Data Exchange (ETDEWEB)

The major focus of this oil-shale investigation has been on specific localities of oil-shale resource potential. Three main areas of oil-shale occurrence have been studied in detail: the Elko area, Pinon Range area, and Coal Mine Canyon. Geologic mapping, stratigraphic studies, and sampling to delimit the lateral extent of the oil shale deposits were in progress prior to the cooperative agreement with Nevada DOE. These surface geologic studies have been summarized in this report. The results of surface geologic studies conducted near Elko suggested that the Elko area represented the best and most accessible oil-shale deposits; therefore, the Elko area was selected as the site of a shallow exploratory drilling program. Essential to this study was the obtaining of fresh, unweathered oil-shale samples from the Elko area. The samples were obtained from the core-drilling program and tested for oil yield by Fischer assays. The oil yields determined from these samples, together with the ...

1983-01-01

461

Preliminary characterization of the F-Area Railroad Crosstie Pile at the Savannah River Site. Revision 1  

Energy Technology Data Exchange (ETDEWEB)

Historical information about the F-Area Railroad Crosstie Pile is limited. The unit is believed to have been a borrow area for earth fill that began receiving railroad crossties during the 1960s. The number of crossties at the unit began to increase significantly in 1984 when major repair of the SRS rail system was initiated. An estimated 100,000 used railroad crossties have accumulated at the unit since 1984. In an effort to determine the impact of the railroad crossties on the environment a total of 28 soil samples were collected from four test borings in March of 1991. Sample depths ranged from ground surface to 21.5 feet. Three of the borings were extended to the water table and groundwater samples were collected, one in an upgradient ``background`` area, and two downgradient from the unit. Few analytes were reported above detection limits. Test results are summarized in Section 4.0 and analytes not detected are summarized in Appendix A to this report. In three ...

1991-10-01

462

Preliminary characterization of the F-Area Railroad Crosstie Pile at the Savannah River Site  

Energy Technology Data Exchange (ETDEWEB)

Historical information about the F-Area Railroad Crosstie Pile is limited. The unit is believed to have been a borrow area for earth fill that began receiving railroad crossties during the 1960s. The number of crossties at the unit began to increase significantly in 1984 when major repair of the SRS rail system was initiated. An estimated 100,000 used railroad crossties have accumulated at the unit since 1984. In an effort to determine the impact of the railroad crossties on the environment a total of 28 soil samples were collected from four test borings in March of 1991. Sample depths ranged from ground surface to 21.5 feet. Three of the borings were extended to the water table and groundwater samples were collected, one in an upgradient background'' area, and two downgradient from the unit. Few analytes were reported above detection limits. Test results are summarized in Section 4.0 and analytes not detected are summarized in Appendix A to this ...

1991-10-01

463

Kansas Vocational Education Performance Report for Fiscal Year 1993. Carl D. Perkins Vocational and Applied Technology Education Act Amendments of 1990.  

Science.gov (United States)

The Kansas State Board of Education awarded $10.25 million to local education agencies through the Carl D. Perkins Vocational and Applied Technology Education Act of 1990. A work group developed the System of Measures and Standards of Performance (SMSP). Programs for single parents, displaced homemakers, and single pregnant women were funded to provide education/training, employment readiness, and job placement. Seven gender equity facilitator projects and 17 projects to provide young women access to training and support services for technical and nontraditional occupations were funded. Funding provided programs for criminal offenders in corrections institutions and special populations students. To promote state leadership and professional development, funds for curriculum development, inservice training, and vocational student organizations were awarded. Community-based organizations provided transitional programs, outreach programs, and other services for economically and ...

1994-01-11

464

Isotope Production at the Hanford Site in Richland, Washington  

Energy Technology Data Exchange (ETDEWEB)

This report was prepared in response to a request from the Nuclear Energy Research Advisory Committee (NERAC) subcommittee on ''Long-Term Isotope Research and Production Plans.'' The NERAC subcommittee has asked for a reply to a number of questions regarding (1) ''How well does the Department of Energy (DOE) infrastructure sme the need for commercial and medical isotopes?'' and (2) ''What should be the long-term role of the federal government in providing commercial and medical isotopes?' Our report addresses the questions raised by the NERAC subcommittee, and especially the 10 issues that were raised under the first of the above questions (see Appendix). These issues are related to the isotope products offered by the DOE Isotope Production Sites, the capabilities and condition of the facilities used to produce these products, the management of the isotope production ...

1999-06-01

465

Improvement of numerical analysis method for FBR core characteristics. 2  

Energy Technology Data Exchange (ETDEWEB)

This report is composed of the following two parts and appendix. (I) Improvement of the Method for Evaluating Reactivity Based on Monte Carlo Perturbation Theory: Theoretical formulation in Monte Carlo perturbation method had been checked, and then introduced into a calculation code. There are some cases that the results of the change of eigenvalues becomes positive or negative by changing the estimator, and there is no reasonable difference in the results between the conventional method, which does not consider the change of neutron source distribution caused by a perturbation, and the new method, which consider that change. Thus it is still necessary to check the Monte Carlo perturbation code. (II) Improvement of Nodal Transport Method for 3-D Hexagonal Geometry: We can accurately evaluate hexagonal geometry FBR core by nodal transport calculation code for hexagonal-Z geometry named `NSHEX`. However it is also found that in very heterogeneous core the results is ...

1997-03-01

466

Federal Facility Agreement plans and schedules for liquid low-level radioactive waste tank systems at Oak Ridge National Laboratory, Oak Ridge, Tennessee  

Energy Technology Data Exchange (ETDEWEB)

The Superfund Amendments and Reauthorization Act of the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) requires a Federal Facility Agreement (FFA) for federal facilities placed on the National Priorities List. The Oak Ridge Reservation was placed on that list on December 21, 1989, and the agreement was signed in November 1991 by the Department of Energy Oak Ridge Field Office (DOE-OR), the US Environmental Protection Agency (EPA)-Region IV, and the Tennessee Department of Environment and Conservation (TDEC). The effective date of the FFA was January 1, 1992. Section 9 and Appendix F of the agreement impose design and operating requirements on the Oak Ridge National Laboratory (ORNL) liquid low-level radioactive waste (LLLW) tank systems and identify several plans, schedules, and assessments that must be submitted to EPA/TDEC for review or approval. The initial issue of this document in March 1992 transmitted to EPA/TDEC those plans ...

1993-06-01

467

Farm & Ranch Business Management. Second Edition.  

Science.gov (United States)

This practical guide for the agribusiness manager (the farmer, rancher, and other agribusiness people who work with agricultural commodities, supplies, and services) gives a basic understanding of modern management practices. It provides guidelines that can help them make practical business decisions. Chapter 1 is an introduction that highlights farm management efficiency, decision making, and goals and objectives. The following 11 chapters discuss recordkeeping (importance and purposes of keeping records, types of systems, record book components), financial analysis (cash flow, net worth, income statements and their ratios; whole farm and enterprise analysis; relationships of various analyses), budget analysis (introduction to types and uses of budgets, how to use and analyze a partial budget, enterprise budget and whole farm budget, production principles, cost components), cash flow analysis (projections and analysis, repayment capacity, credit needs, credit types and sources), ...

1986-12-01

468

Facilities Condition and Hazards Assessment for Materials and Fuel Complex Facilities MFC-799, 799A, and 770C  

Energy Technology Data Exchange (ETDEWEB)

The Materials & Fuel Complex (MFC) facilities 799 Sodium Processing Facility (a single building consisting of two areas: the Sodium Process Area (SPA) and the Carbonate Process Area (CPA), 799A Caustic Storage Area, and 770C Nuclear Calibration Laboratory have been declared excess to future Department of Energy mission requirements. Transfer of these facilities from Nuclear Energy to Environmental Management, and an associated schedule for doing so, have been agreed upon by the two offices. The prerequisites for this transfer to occur are the removal of nonexcess materials and chemical inventory, deinventory of the calibration source in MFC-770C, and the rerouting and/or isolation of utility and service systems. This report provides a description of the current physical condition and any hazards (material, chemical, nuclear or occupational) that may be associated with past operations of these facilities. This information will document conditions at time of transfer of the ...

2009-11-01

469

Evaluation of advanced technologies for residential appliances and residential and commercial lighting  

Energy Technology Data Exchange (ETDEWEB)

Section 127 of the Energy Policy Act requires that the Department of Energy (DOE) prepare a report to Congress on the potential for the development and commercialization of appliances that substantially exceed the present federal or state efficiency standards. Candidate high-efficiency appliances must meet several criteria including: the potential exists for substantial improvement (beyond the minimum established in law) of the appliance`s energy efficiency; electric, water, or gas utilities are prepared to support and promote the commercialization of such appliances; manufacturers are unlikely to undertake development and commercialization of such appliances on their own, or development and production would be substantially accelerated by support to manufacturers. This report describes options to improve the efficiency of residential appliances, including water heaters, clothes washers and dryers, refrigerator/freezers, dishwashers, space heating and cooling devices, as well as ...

1995-01-01

470

Development of a stable cobalt-ruthenium Fischer-Tropsch catalyst. Technical progress report No. 12, July 1, 1992--September 30, 1992  

Energy Technology Data Exchange (ETDEWEB)

The fixed bed pilot plant, the catalyst testing procedure, and the calculations for conversion and selectivities were previously described in the technical progress report covering the period of 3/16/88 to 6/16/88 for Contract DE-AC22-87PC79812. Conversions and hydrocarbon selectivities were calculated using data from an on-line gas chromatography (GC) analyzer. Alcohol selectivities were calculated using data from an on-line boiling point GC analyzer which analyzed the liquid product. The catalysts were prepared via the steps of impregnation, calcination, and reduction on a special Y-zeolite-derived support. The impregnation step consisted of evaporation of metal salts on to the support from an aqueous solution. For one catalyst (No. 6531-188) the metal salts were evaporated on to the support from a reverse micelle solution containing the metal salts. All the catalysts were calcined for four hours at 450{degree}C. The calcined catalysts were loaded in the reactor with a diluent ...

1992-12-31

471

Coal gasification via the Lurgi process: Topical report: Volume 1, Production of SNG (substitute material gas)  

Energy Technology Data Exchange (ETDEWEB)

A Lurgi baseline study was requested by the DOE/GRI Operating Committee of the Joint Coal Gasification Program for the purpose of updating the economics of earlier Lurgi coal gasification plant studies for the production of substitute natural gas (SNG) based on commercially advanced technologies. The current study incorporates the recent experience with large size Lurgi plants in an effort to improve capital and operating costs of earlier plant designs. The present coal gasification study is based on a mine mouth plant producing 250 billion Btu (HHV) per day of SNG using the Lurgi dry bottom coal gasification technology. A Western subbituminous coal was designated as the plant food, obtained from the Rosebud seam at Colstrip, Montana. This study presents the detailed description of an integrated facility which utilizes coal, air, and water to produce 250 billion Btu (HHV) per day of SNG. The plant consists of coal handling and preparation, twenty-six Lurgi dry bottom gasifiers, shift ...

1984-09-01

472

BMBF. The biotechnology 2000 project. Annual report 1994; BMBF. Programm Biotechnologie 2000. Jahresbericht 1994  

Energy Technology Data Exchange (ETDEWEB)

The `biotechnology 2000` project is supervised by the project sponsor `biology, energy, ecology` at Forschungszentrum Juelich on behalf of the Federal Ministry for Research and Technology. The project activities cover the development of techniques and methods, cell biology, gene structure and gene regulation, photosynthetic production processes, biological hydrogen production, synthetic biology, protein design, neurobiological research, biosystems, plant breeding, phytomedicine and plant protection, the biology of waste disposal methods, research into methods which replace animal experiments, biological safety research, technology risk assessment, and ethical aspects. A general survey introduces the promoted projects, and standardized data sheets briefly introduce the individual activities. The appendix gives the project indices, the indices of joint projects, a list of the supported companies and institutions, and the project sponsor flowsheet. (orig.) [Deutsch] ...

1995-11-01

473

Additional protocol between France, EURATOM and IAEA. 2001-2002 ordinary session. Project of law authorizing the ratification of the additional protocol to the agreement between France, the European Atomic Energy Community and the International Atomic Energy Agency relative to the application of warranties in France; Protocole additionnel entre la France, Euratom et l'AIEA. Session ordinaire de 2001-2002. Projet de loi autorisant la ratification du protocole additionnel a l'accord entre la France, la Communaute europeenne de l'energie atomique et l'Agence internationale de l'energie atomique relatif a l'application de garanties en France  

Energy Technology Data Exchange (ETDEWEB)

This additional protocol to the agreement between France, EURATOM and the IAEA aims at reinforcing the nuclear weapons non-proliferation regime. This protocol widens the field of competences of the IAEA with the supply of new information relative to: the civil nuclear cooperation between France and countries having no nuclear weapons in the domain of fuel cycle; the regular inspection of French nuclear facilities; the trade (import and export) of medium- or high-level radioactive wastes containing plutonium, highly enriched uranium or {sup 233}U, and the trade of some non-nuclear equipments or materials with countries having no nuclear weapons. The protocol defines also some practical dispositions relative to the delays and periodicity of controls, to the transmission of data, to the appointment of IAEA inspectors and their access to the facilities and sites considered. The list of the activities and equipments covered by the protocol are given in appendixes. ...

2002-07-01

474

On the impact of low power density microwaves in some living tissues  

Energy Technology Data Exchange (ETDEWEB)

The biomedical and ecological interest for the microwave impact on the Earth biosphere is continuously increased since the industrial, military and communication activities strongly contribute to the electromagnetic stress of living bodies. In the next the authors present some of the main results obtained regarding the microwave exposure of various types of biological material: bacteria, fungi, young plant seedlings, dry seeds, animal tissues. The electromagnetic exposure was carried out in open space in well controlled environmental conditions by using 10.75 GHz/1 m W cm{sup -2} microwaves. Biochemical assays and cytogenetic tests have been carried out to reveal the changes induced post irradiation. The response of some pathogen bacteria, have been emphasized by means of turbidimetric measurements - the stimulatory effect being noticed at the level of the microbial population density (the stimulation of the human body microbial flora seems to ...

2006-07-01

475

Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat.  

Science.gov (United States)

In acute liver failure following hepatitis, toxic insults, or after major liver surgery, there is an increased bacterial translocation from the gut. This may explain some of the infectious complications seen in these conditions. To elucidate mechanisms and find possible preventive measures, we investigated the effect of rectal administration of arginine and probiotic bacteria (Lactobacillus spp.) on bacterial translocation and the extent of liver failure. Sprague-Dawley rats were used and five different Lactobacillus strains (Lb. reuteri R2LC, Lb. rhamnosus DSM 6594 (= strain 271), Lb. plantarum DSM 9843 (= strain 299v), Lb. fermentum 8704:3 (= strain 245), and Lb. reuteri (= strain 108) were administered rectally once daily for 8 days with and without 2% arginine. Acute liver injury (ALI) was induced on the eighth day by intraperitoneal injection of D-galactosamine (1.1 g/kg body weight), and samples were collected after 24 and 48 hours. Bacterial translocation ...

1997-03-01

476

Phenomenology of the #LAMBDA#/#SIGMA#production ratio in pp collisions  

International Nuclear Information System (INIS)

We show that the recently measured asymmetry in helicity-angle spectra of the #LAMBDA#-hyperons, produced in the reaction pp#->#K"+#LAMBDA#p reaction, and the energy dependence of the total pp#->#K"+#LAMBDA#p cross-section can be explained consistently by the same #LAMBDA#p final-state interaction. Assuming that there is no final-state interaction in the #SIGMA#"0 p channel, as suggested by the available data for the reaction pp#->#K"+#SIGMA#"0p, we can also reproduce the energy dependence of the #LAMBDA#/#SIGMA#"0 production ratio and, in particular, the rather large ratio observed near the reaction thresholds. The nominal ratio of the #LAMBDA# and #SIGMA#"0 production amplitudes squared, i.e. when disregarding the final-state interaction, turns out to be about 3, which is in line with hyperon production data from proton and nuclear targets available at high energies. (orig.)

2006-09-01

477

Identification of model parameters of excitation system and power system stabilizer of Mingtan{number_sign}6 via finalization field tests  

Energy Technology Data Exchange (ETDEWEB)

Experiences with the model parameter identification of the excitation system (EXS) and the power system stabilizer (PSS) for Mingtan{number_sign}6 pumped storage generation unit of Taiwan Power System are presented in this paper. The input-output data corresponding to each block of the EXS and PSS were obtained when the finalization tests of this unit were performed. The generalized least squares (GLS) approach is introduced and employed to identify the desired parameters of the noisy excitation system and PSS models. In this method, the reduction technique of biased estimates due to the non-white (correlated) identification residual is also applied to improve the accuracy of identification. The results of the parameter estimation are satisfactory. The GLS parameter identification method using the measured data at finalization tests is then suggested from the viewpoint of economy and engineering.

1995-05-01

478

Amorphous cellulose gel as a fat substitute in fermented sausages  

British Library Electronic Table of Contents (United Kingdom)

Fermented sausages were produced with 25%, 50%, 75% or 100% of their pork back fat content replaced by amorphous cellulose gel. The sausage production was monitored with physical, chemical and microbiological analyses. The final products were submitted to a consumer study, and the volatile compounds of the final products were extracted by solid-phase microextraction and analyzed by GC/MS. The reformulated fermented sausages had significant reductions in fat and cholesterol, and the volatile compounds derived from lipid oxidation were also reduced in the final products. These results suggest that the substitution of up to 50% of the pork back fat content by amorphous cellulose gel can be accomplished without a loss of product quality, enabling the production of fermented sausages with the l...

2012-01-01

479

Transdermal Drug Delivery System. Stage 1. Volume 1.  

Science.gov (United States)

... Accession Number : ADB119274. Title : Transdermal Drug Delivery System. Stage 1. Volume 1. Descriptive Note : Final rept. 15 Aug 85-14 Oct 87,. ...

1987-12-30

480

Storage of buffy-coat-derived platelets in additive solution: in vitro effects on platelets of the air bubbles and foam included in the final unit  

UK PubMed Central (United Kingdom)

Background.The air bubbles and foam that develop during the preparation of platelet units have traditionally been considered to interact with the platelets, causing activation and...Full Text Available

2011-04-01

481

Specific Residues of the Influenza A Virus Hemagglutinin Viral RNA Are Important for Efficient Packaging into Budding Virions?  

UK PubMed Central (United Kingdom)

A final step in the influenza virus replication cycle is the assembly of the viral structural proteins and the packaging of the eight segments of viral RNA (vRNA) into a fully infectious virion. The...Full Text Available

2007-09-01

482

SG Report on Harmony with Nature final single-spaced.doc  

Wastenet

of early humanitarians, and the vivisectors turned to Rene Descartes (1596 1650 ) to justify ...A celebrated mathematician, physiologist, and psychologist, Descartes provided a general philosophy of the irrelevance of ethics to the ...Animals, according to Descartes, were insensible and irrational machines; moved, like clocks, but

483

ROCK DEFORMATION. Final Progress Report  

Energy Technology Data Exchange (ETDEWEB)

The Gordon Research Conference (GRC) on ROCK DEFORMATION was held at II Ciocco from 5/19/02 thru 5/24/02. Emphasis was placed on current unpublished research and discussion of the future target areas in this field.

2002-05-24

484

Programmed Cell Death during Pollination-Induced Petal Senescence in Petunia1  

UK PubMed Central (United Kingdom)

Petal senescence, one type of programmed cell death (PCD) in plants, is a genetically controlled sequence of events comprising its final developmental stage. We characterized the pollination-induced...Full Text Available

2000-04-01

485

PAMELA data and leptonically decaying dark matter  

CERN Document Server

Recently PAMELA released their first results on the positron and antiproton ratios. Stimulated by the new data, we studied the cosmic ray propagation models and calculated the secondary positron and antiproton spectra. The low energy positron ratio can be consistent with data in the convection propagation model. Above $\\sim 10$ GeV PAMELA data shows a clear excess on the positron ratio. However, the secondary antiproton is roughly consistent with data. The positron excess may be a direct evidence of dark matter annihilation or decay. We compare the positron and anti-proton spectra with data by assuming dark matter annihilates or decays into different final states. The PAMELA data actually excludes quark pairs being the main final states, disfavors gauge boson final states. Only in the case of leptonic final states the positron and anti-proton spectra can be explained simultaneously. We also compare the ...

2008-01-01

486

NRC regulatory agenda: Semiannual report, January--June 1997. Volume 16, Number 1  

Energy Technology Data Exchange (ETDEWEB)

The Regulatory Agenda is a semiannual compilation of all rules on which the NRC has recently completed action, or has proposed action, or is considering action, and of all petitions for rulemaking that the NRC has received that are pending disposition. The agenda consists of two sections that have been updated through June 30, 1997. Section 1, ``Rules,`` includes (A) rules on which final action has been taken since December 31, 1996, the closing date of the last NRC Regulatory Agenda; (B) rules published previously as proposed rules on which the Commission has not taken final action; (C) rules published as advance notices of proposed rulemaking for which neither a proposed nor final rule has been issued; and (D) unpublished rules on which the NRC expects to take action. Section 2, ``Petitions for Rulemaking,`` includes (A) petitions denied or incorporated into final rules since December 31, 1996; (B) ...

1997-08-01

487

KJNWFZ Concept Paper May6-2010  

Wastenet

KOREA-JAPAN NUCLEAR WEAPON FREE ZONE (KJNWFZ) CONCEPT PAPER ...A Korea-Japan2 Nuclear Weapon Free Zone (hereafter KJNWFZ) is a new concept.Once realized, ...Finally, we outline the key elements of the Korea-Japan NWFZ proposed in this paper, noting

488

Introduction of a Secondary Blast Process into an Unlined Hot-Blast Cupola Furnace. Final Report.  

Science.gov (United States)

In a series of comprehensive tests lasting 18 months the relationship between the CO-content of the waste gas from a hot-blast cupola furnace and the amount of secondary blast was determined. The additional CO-combustion by means of the secondary blast an...

1986-01-01

489

Fused Silica Final Optics for Inertial Fusion Energy: Radiation Studies and System-Level Analysis  

International Nuclear Information System (INIS)

The survivability of the final optic, which must sit in the line of sight of high-energy neutrons and gamma rays, is a key issue for any laser-driven inertial fusion energy (IFE) concept. Previous work has concentrated on the use of reflective optics. Here, we introduce and analyze the use of a transmissive final optic for the IFE application. Our experimental work has been conducted at a range of doses and dose rates, including those comparable to the conditions at the IFE final optic. The experimental work, in conjunction with detailed analysis, suggests that a thin, fused silica Fresnel lens may be an attractive option when used at a wavelength of 351 nm. Our measurements and molecular dynamics simulations provide convincing evidence that the radiation damage, which leads to optical absorption, not only saturates but that a 'radiation annealing' effect is observed. A system-level description is provided, including ...

2003-06-01

490

Feasibility Study for Integrated Tax Management System (Kenya) (Final Report, Public Version, Revised).  

Science.gov (United States)

The Kenya Revenue Authority (KRA) has been engaged in revenue administration reform for several years. Among the KRA's impressive, recent accomplishments is the integration of formerly separate departments for income taxes and value added taxes. (VAT) und...

2006-01-01

491

Extraction of the weak angle gamma from B to charm decays  

CERN Document Server

We give a summary of the discussions in Working Group V of the CKM2010 workshop dealing with determinations of the angle gamma of the unitarity triangle of the Cabibbo-Kobayashi-Maskawa matrix from B-meson decays into charmed final states.

2011-01-01

492

Environmental assessment of the Alaskan continental shelf. Final reports of principal investigators, Volume 15. Biological studies  

Energy Technology Data Exchange (ETDEWEB)

The contents of the report include: Identification, documentation and delineation of coastal migratory bird habitat; and an annotated bibliography of literature on Alaska Water Birds.

1981-12-01

493

Development of a Coke-Free Cupola Furnace. Energy Conserving Shaft Furnace for Iron Materials. Final Report.  

Science.gov (United States)

Coke-fired cupola furnaces were improved and made suitable for the production of high-quality casting melts by numerous additional devices. Moreover, they were equipped with environmental protection systems with numerous dust separation devices and afterb...

1986-01-01

494

Demonstration of Black Liquor Gasification at Big Island  

Energy Technology Data Exchange (ETDEWEB)

This Final Technical Report provides an account of the project for the demonstration of Black Liquor Gasification at Georgia-Pacific LLC's Big Island, VA facility. This report covers the period from May 5, 2000 through November 30, 2006.

2007-04-14

495

COMPUTER ECONOMICS OF PHYSICAL COAL CLEANING AND FLUE GAS DESULFURIZATION. FINAL REPORT  

Science.gov (United States)

The report describes a computer model developed by TVA to simulate the performance and determine the economics of coal cleaning, or coal cleaning combined with flue gas desulfurization (FGD), for power plant emission control processes over a wide range of user-specified condition...