UK PubMed Central (United Kingdom)

A Legionella-like organism (strain 1087-AZ-H) was isolated from a pleural-fluid specimen from a renal transplant patient undergoing immunosuppressive therapy. Growth characteristics and gas-liquid chromatography...Full Text Available

UK PubMed Central (United Kingdom)

Three Legionella-like organisms were isolated from water from the cooling towers of two Australian institutions. The strains grew on buffered charcoal-yeast extract (BCYE) agar but not on BCYE agar...Full Text Available

UK PubMed Central (United Kingdom)

Mycoplasma pneumoniae is a common etiologic agent of lower respiratory tract infections in humans. However, it has been reported previously that the organism has occasionally been isolated from sites...Full Text Available

UK PubMed Central (United Kingdom)

Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium...Full Text Available

UK PubMed Central (United Kingdom)

The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections...Full Text Available

UK PubMed Central (United Kingdom)

Coryneform isolates from clinical specimens frequently cannot be identified by either reference laboratories or research laboratories. Many of these organisms are skin flora that belong to a large number...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

The concentrates of scattered organic matter???kerogen-like polymer, which is insoluble in acids and organic solvents???were isolated from rock samples taken at different depths from various stratigraphic units at the productive deposits of the Orenburg oil-gas condensate field using chemical methods. The chemical composition of the kerogen-like polymer and the concentrations of trace elements in it were studied. The regularities of evolutionary changes in the composition of the organic matter and the concentrations of trace elements in it in the order of the kerogen-like polymer and the following its consecutive derivatives were established: asphaltenes, ethanol-benzene tars, benzene tars, oils, and paraffins

Energy Technology Data Exchange (ETDEWEB)

In an effort to improve the extrapolation of laboratory data to man and estimate risk of human reproductive toxicity associated with environmental exposure, the pharmacokinetic parameters of the testicular compartment are being studied. Of particular interest is the variety of enzyme systems capable of activating and detoxicating environmental chemicals and drugs. This report compares the metabolism of benzo(a)pyrene by the isolated perfused testis and testicular homogenates in vitro. The cell free in vitro system metabolized benzo(a)pyrene at a much greater rate than the perfused testis and produced a different spectrum of metabolites. Reliable laboratory prediction of biotransformation by the whole organ or intact animal is an essential aspect of reproductive toxicology.

British Library Electronic Table of Contents (United Kingdom)

Mung bean seedlings inoculated with Enterobacter asburiae PSI3, a gluconic acid-producing rhizosphere isolate, enhanced plant growth in the presence of phytotoxic levels of Cd2+ in gnotobiotic pot experiments as compared to the uninoculated Cd-treated plants. Addition of organic acids to Cd-stressed seedlings promoted root elongation. Hematoxylin competition assays showed that organic acids could displace Cd2+ from the Cd2+: hematoxylin complex in the same order of effectiveness as was found for restoration of root net elongation viz. oxalate?>?malate?>?succinate while gluconate was effective at higher concentrations. Root associated Cd2+, assessed by hematoxylin staining of roots was found to be reduced when roots were treated with organic acid. Cd stress increased antioxidant enzymes suc...

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

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Our major goal of our work has been to develop and use NMR techniques to study how methanogenic archaebacteria deal with osmotic stress with the hope of providing insights into increasing the salt tolerance of other cells. The project has three main sections: (i) in vivo studies of methanogens; (ii) use of [sup l3]C- and [sup l5]N- labeled potential precursors and in vitro analyses of specific label uptake for elucidation of osmolyte dynamics and biosynthetic pathways of osmolytes in these organisms, and isolation of key biosynthetic enzymes; and (iii) collaborative studies on identification of organic solutes in other methanogens.

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Some 43 papers were presented in 4 sections: Organ and bud culture of tree species (24 papers); Cell suspension and callus culture of tree species (11); Culture of haploid tissue of tree species and production of wood homozygotes (3); and Isolation, culture and somatic fusion of protoplasts as a means of somatic hybridization and genetic engineering (5).

UK PubMed Central (United Kingdom)

The abundant microbial population in a 3,043-m-deep Greenland glacier ice core was dominated by ultrasmall cells (<0.1 μm³) that may represent intrinsically small organisms...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

In order to study the species composition of endophytes from wheat healthy plants in Buenos Aires Province (Argentina) and to determine their infection frequencies from leaves, stems, glumes and grains, wheat plants were collected from five cultivars at five growth stages from crop emergence to harvest. A total of 1,750 plant segments (leaves, stems, glumes and grains) were processed from the five wheat cultivars at five growth stages, and 722 isolates of endophytic fungi recovered were identified as 30 fungal genera. Alternaria alternata, Cladosporium herbarum, Epicoccum nigrum, Cryptococcus sp., Rhodotorula rubra, Penicillium sp. and Fusarium graminearum were the fungi that showed the highest colonization frequency (CF%) in all the tissues and organs analysed. The number of taxa isolated...

International Nuclear Information System (INIS)

Atrazine and phenanthrene (Phen) sorption by nonhydrolyzable carbon (NHC), black carbon (BC), humic acid (HA) and whole sediment and soil samples was examined. Atrazine sorption isotherms were nearly linear. The single-point organic carbon (OC)-normalized distribution coefficients (K_O_C) of atrazine for the isolated HA1, NHC1 and BC1 from sediment 1 (ST1) were 36, 550, and 1470 times greater than that of ST1, respectively, indicating the importance of sediment organic matter, particularly the condensed fractions (NHC and BC). Similar sorption capacity of atrazine and Phen by NHC but different isotherm nonlinearity indicated different sorption domains due to their different structure and hydrophobicity. The positive relationship between (O + N)/C ratios of NHC and atrazine log K_O_C at low concentration suggests H-bonding interactions. This study shows that sediment is probably a less effective sorbent for atrazine than ...

British Library Electronic Table of Contents (United Kingdom)

There is a thermal range for the operation of neural circuits beyond which nervous system function is compromised. Locusta migratoria is native to the semiarid regions of the world and provides an excellent model for studying neural phenomena. In this organism previous exposure to sublethal high temperatures (heat shock, HS) can protect neuronal function against future hyperthermia but, unlike many organisms, the profound physiological adaptations are not accompanied by a robust increase of Hsp70 transcript or protein in the nervous system. We compared Hsp70 increase following HS in the tissues of isolated and gregarious locusts to investigate the effect of population density. We also localized Hsp70 in the metathoracic ganglion (MTG) of gregarious locusts to determine if HS affects Hsp70 ...

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The black carbon continuum is composed of a series of carbon-rich components derived from combustion or metamorphism and characterized by contrasting environmental behavior and susceptibility to oxidation. In this work, we present a micro-scale density fractionation method that allows isolating the small quantities of soot-like and graphitic material usually found in natural samples. Organic carbon and {delta}{sup 13}C mass balance calculations were used to quantify the relative contributions of the two fractions to thermally-stable organic matter from a series of aquatic sediments. Varying proportions of soot-like and graphitic material were found in these samples, with large variations in {delta}{sup 13}C signatures suggesting important differences in their origin and/or dynamics in the environment.

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In the Assel'skiy and Sakmarskiy time of the lower Permian epoch, on the territory of the modern Dnieper and Donets, and Pripyatskiy Basins, there was a gulf which stretched in a northwest direction. The latter connected to the open sea in the east through the CisDonets trough. Transverse tectonic elevations divided the gulf into 5 semi-isolated reservoirs. In the Assel'skiy time, in the period of carbonate sedimentation, the development of algae, crinoids, corals and other organisms occurred. They created reef, bioherm and biostroma reconstructions. The most favorable sections for their settlement were the coastal zones of the gulf, consedimentation positive structures and transverse tectonic elevations. It is assumed that the formation of reefs, bioherms and biostromas, on the one hand, and sedimentation of evaporites on the other hand, are interrelated processes. The first after the next marine transgression during their ...

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International Science & Technology Center (ISTC)

Isolation and Identification of Mycoplasmas, Development of Reliable Antigen for Mycoplasmas Detection and Oil-Based Vaccines

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Lacustrine rocks are a significant component of many rift-valley sequences. Comparisons of both active and ancient rift valleys indicate that the lacustrine facies are commonly rich in organic matter and may be important sources for oil. For example, Holocene sediments in Lake Tanganyika and Cretaceous lacustrine rocks in west Africa contain as much as 12% and 20% TOC, respectively. The Newark Supergroup contains abundant lacustrine rocks. The widespread occurrence of black shales, the general similarity to known organically rich rift systems, and a few isolated geochemical analyses have caused some speculation about the potential of the Newark Supergroup to be an effective source of oil and gas. Sufficient geochemical analyses are available from lacustrine rocks in the Newark, Connecticut, and Deep River basins to evaluate their potential as hydrocarbon sources. In general, both the quantity and quality of ...

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A novel, obligately anaerobic, extremely thermophilic, cellulolytic bacterium, designated OB47T, was isolated from Obsidian Pool, Yellowstone National Park, WY, USA. The isolate was a non-motile, non-spore forming, Gram-positive rod approximately 2 m long by 0.2 m wide and grew at temperatures between 55-85oC with the optimum at 78oC. The pH range for growth was 6.0-8.0 with values of near 7.0 being optimal. Growth on cellobiose produced the fastest specific growth rates at 0.75 hr-1. The organism also displayed fermentative growth on glucose, maltose, arabinose, fructose, starch, lactose, mannose, sucrose, galactose, xylose, arabinogalactan, Avicel, xylan, filter paper, processed cardboard, pectin, dilute acid-pretreated switchgrass and Populus. OB47T was unable to grow on mannitol, fucose, lignin, Gelrite, acetate, glycerol, ribose, sorbital, carboxymethylcellulose and casein. Yeast extract stimulated growth and ...

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Assimilation and metabolism of /sup 14/CO/sub 2/ was compared to that of (U-/sup 14/C) sucrose in young grapefruit (ca 25 mm diameter) to determine their respective roles in fruit growth. Fixation of /sup 14/CO/sub 2/ by isolated fruit tissues during 10 min in light exceeded that in dark by 2- to 30-fold depending on tissue content of chlorophyll. Greatest apparent photosynthesis occurred in outer green peel, but green juice tissues assimilated more than did adjoining inner peel tissue. In the dark, juice tissues incorporated 2.5-fold more /sup 14/CO/sub 2/ than any other tissue. Neutral sugars accounted for a smaller proportion and organic acids, a greater proportion, of the /sup 14/C-assimilates in interior peel and juice tissues. These data suggest more extensive production of organic acids from /sup 14/CO/sub 2/ in tissues isolated from the fruit interior. In contrast, little difference among ...

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Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.

British Library Electronic Table of Contents (United Kingdom)

Fluoroalkyl end-capped vinyltrimethoxysilane oligomer suffered the sol-gel reaction under alkaline conditions in the presence of titanium oxide nanoparticles in tetrahydrofuran to afford the corresponding fluorinated oligomer/titanium oxide nanocomposites[RF-(VM-SiO2)n-RF/TiO2] in excellent to moderate isolated yields. These fluorinated composites thus obtained were nanometer size-controlled fine particles, and exhibited good dispersibility and stability in traditional organic solvents except for water. These fluorinated nanocomposites were applied to the surface modification of glass to exhibit not only a completely superhydrophobic characteristic (a water contact angle: 180degree) with a non-wetting property against water droplets but also a good oleophobicity imparted by fluoroalkyl seg...

British Library Electronic Table of Contents (United Kingdom)

Mediterranean mariculture uses imported strains of marine phytoplankton, raising questions of ecological risk and ability to adapt to local conditions for mass culture outdoors. In this context, we report here on the mass-culture potential and chemical composition of six strains of Prasinophyceae (five strains of Tetraselmis sp. and one Pyramimonas sp.) isolated from a Greek coastal lagoon. Proximate composition had a pattern of 10?20% ash, 35?65% protein, 6?10% lipids, and 25?45% other organics including carbohydrates. The amino acid profiles were typical for the marine representatives of the class. All strains had a high PUFA content with dominant the ?3 fraction in four of them. The fatty acid profiles indicated a Tetraselmis strain with high EPA (14%) and a Pyramimonas strain with high...

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Recently the authors showed that tetrazolium salts can be obtained during the oxidation of substituted 1,3,5-triarylformazans with potassium permanganate in a two-phase organic solvent-water system. The role of phase-transfer catalyst in this reaction is played by the tetrazolium salt, which is formed in a small amount as the result of oxidation of the formazan at the phase boundary. The method is distinguished by its extreme simplicity. However, the yield of the tetrazolium salts fluctuates within wide limits and does not exceed 62%. This is due to the fact that as the reaction proceeds the pH of the aqueous phase increases from 6 to 12. At the same time it is known that tetrazolium salts are unstable in aqueous alkaline solutions. They found that if the aqueous phase is replaced by aqueous hydrochloric acid (5 wt. %) the yields of the tetrazolium salts (Ia-g) are increased to 70-87%. For convenience of isolation the readily water-soluble ...

Science.gov (United States)

Polysomaty analysis of the succulent portulaca (Portulaca grandiflora Hook.) plant was carried out using flow cytometry. For both diploid and tetraploid plants, mature leaf tissue was found to have a higher level of polysomaty than young leaf tissue. Mesophyll (MP), bundle sheath (BSP) and water storage protoplasts (WSP) were isolated from leaf tissues of diploid portulaca plants. WSP had a higher degree of endopolyploidization than MP and BSP. The ploidy distribution was also variable in different floral organs. Tetraploid plants artificially induced by colchicine treatment showed a decline in the degree of polysomaty compared to diploid plants. Tetraploid plants had more spherical leaves, a larger number of petals and lower pollen fertility than diploid plants. PMID:10936528

Science.gov (United States)

A practical hands-on course encompassing enzyme purification, biochemical characterization, and crystallization that completed the course work of 350 second-year bachelor students enrolled in molecular biology/biochemistry was given at the Universite Louis Pasteur of Strasbourg (France). The experimental part of the practical dealt entirely with the model protein lysozyme isolated from hen egg-white. It was designed as a research project to give students the possibility to practice biochemical methods such as chromatography, electrophoresis, and spectrophotometry. It also included enzyme activity assay and protein crystal growth that are usually taught in master-level courses. The organization of the practical work and the related experimental procedures are described and discussed.

British Library Electronic Table of Contents (United Kingdom)

In the present study, we report the occurrence of Lyme's borreliosis in patients from the Brazilian Amazon Region. Borreliosis was investigated by immunohistochemistry and focus floating microscopy for Borrelia burgdorferi in skin biopsy samples from 22 patients with both clinical and histopathology evidences compatible with Erythema Migrans. Spirochetes were detected by specific immunohistochemistry and focus floating microscopy for B. burgdorferi in samples from five patients. Clinical cure of the cutaneous lesions was observed in all the patients after treatment with doxycycline regimen as proposed by the Center Disease Control guidelines. A limitation of our study was the fact that we were not able to isolate and culture these organisms. These are the first known Brazilian cases of bor...

British Library Electronic Table of Contents (United Kingdom)

The objective of this study was to evaluate the association between the number of blood cultures collected and the appropriateness of care for suspected bacteremic community-acquired urinary tract infection (UTI) in the elderly. We retrospectively evaluated the medical records of 129 patients with UTI >65?years old admitted to a large community-based training hospital in Japan from 1 January 2006 to 31 December 2009. We assessed the association between the number of blood cultures collected and the appropriateness of care received, as well as other factors. Two-thirds of the patients were women, and patients >85?years old accounted for 45.0% of the cases. Most of the organisms isolated from the urine and blood were Escherichia coli (65.4?67.0%). More than two blood cultures were collected ...

CERN Document Server

Integrating mission and strategy for nonprofit organizations

British Library Electronic Table of Contents (United Kingdom)

The effects of temperature and different forms of nutrients on Chattonella marina growth have been investigated in strains isolated from the Daya Bay, the South China Sea. The strain of C. marina preferred high temperatures, with an optimal temperature of 25?C, and 18?C was the minimum for its survival. Higher cell number and growth rate were obtained in high nitrogen and phosphorus concentrations (500 ?g/L, 74 ?g/L) than under nutrient limitation. Nitrogen influenced the growth most, as the specific growth rate and maximum cell density were lower in nitrogenlimited cultures than noted under phosphorus limitation or under limitation from both. C. marina was capable of using many kinds of organic nitrogen sources including L-serine (L-Ser), glycine (Gly), alanine (Ala), L-threonine (L-Thr),...

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No abstract prepared.

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UK PubMed Central (United Kingdom)

The vomeronasal organ is the primary olfactory organ that detects sexual pheromones in mammals. We investigated the anatomy of the vomeronasal organ of the tammar wallaby (Macropus eugenii),...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Several lanthanide shift reagents (LSR) were synthesized from praseodymium, europium, dysprosium, and terbium using ethylenediaminetetraacetic acid, trans-1,2-diaminocyclohexane-N,N,N[prime],N[prime]-tetraacetic acid, and diethylenetriaminepentaacetic acid for the purpose of separating overlapping [sup 31]P FT-NMR spectral signals of dissolved organic phosphorus compounds present in concentrated lake-water samples. The extent of change in signal position and line width of several known phosphorus compounds dissolved in pure water was examined as a function of solution pH and the molar ratio of LSR to phosphorus. Praseodymium ethylenediaminetetraacetate (PrEDTA) was determined to be the most effective LSR. Concentrated lake-water samples mixed with PrEDTA were examined with [sup 31]P FT-NMR. The use of PrEDTA provided separation of overlapping signals, indicated that phosphorus compounds were becoming isolated from the solution environment ...

DEFF Research Database (Denmark)

Wharton's jelly contains large amounts of hyaluronic acid, and glucosamine is an important constituent of this macromolecule. In order to evaluate the placental vascular effects of this aminosugar, small chorionic and stem villous arteries were dissected from placental specimens obtained at normal term vaginal deliveries (n = 15). Ring preparations were mounted in organ baths, and isometric wall tensions were measured. Glucosamine and its epimer galactosamine (5 X 10(-4) to 10(-2) M) produced marked relaxation of contractions induced by PGF2 alpha (10(-5) M) in both chorionic and stem villous arteries. The effect was unchanged after pretreatment with atropin, propranolol and indomethacin. The relaxant effect of the neutral sugar mannose was less pronounced compared with that of the hexosamines. Total tissue concentrations of placental hexosamines have been reported within the range needed to produce placental vascular relaxation in the present study. However, the ...

British Library Electronic Table of Contents (United Kingdom)

A variety of platinum(II) complexes of methimazole (2-mercapto-1-methylimidazole; HImS=neutral form and ImS=thiolate form), coordinated in both thione and thiolate forms, have been isolated by reacting methimazole with [PtCl(terpy)]Cl (terpy=2,2prime:6prime,2Prime terpyridine), [PtCl2(bipy)] (bipy=bipyridine), [PtCl2(o-phen)] (o-phen=o-phenanthroline), [PtCl2(CH3CN)2] and [PtCl2(COD)] (COD=1,5-cyclooctadiene). These complexes were characterized by electronic absorption, IR and NMR (1H, 13C, 195Pt) spectroscopies. Molecular structure of [Pt(bipy)(HImS)2]Cl23H2O (3a3H2O) has been established by single crystal X-ray crystallography. Platinum thiolate complex, [Pt(ImS)2(HImS)2] (5), could be obtained by treatment of [Pt(HImS)4]Cl2 with sodium methoxide in methanol. The solution of 5 in organic...

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Phillips Petroleum Company recently completed a microbial selective plugging (MSP) pilot at the North Burbank Unit (NBU), Shidler, Oklahoma. Nutrients were selected for the pilot that could stimulate indigenous microflora in the reservoir brine to grow and produce exopolymer. It was found that soluble corn starch polymers (e.g., maltodextrins) stimulated the indigenous bacteria to produce exopolymer, whereas simple sugars (e.g., glucose and sucrose), as well as complex media (e.g., molasses and Nutrient Broth), did not. Injection of maltodextrin into rock cores in the presence of indigenous NBU bacteria resulted in stable permeability reductions (> 90%) across the entire length, while injection of glucose resulted only in face plugging. In addition, it was found that organic phosphate esters (OPE) served as a preferable source of phosphorus for the indigenous bacteria, since orthophosphates and condensed phosphates precipitated in NBU brine at reservoir ...

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Actinosynnema mirum Hasegawa et al. 1978 is the type species of the genus, and is of phylogenetic interest because of its central phylogenetic location in the Actino-synnemataceae, a rapidly growing family within the actinobacterial suborder Pseudo-nocardineae. A. mirum is characterized by its motile spores borne on synnemata and as a producer of nocardicin antibiotics. It is capable of growing aerobically and under a moderate CO2 atmosphere. The strain is a Gram-positive, aerial and substrate mycelium producing bacterium, originally isolated from a grass blade collected from the Raritan River, New Jersey. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first complete genome sequence of a member of the family Actinosynnemataceae, and only the second sequence from the actinobacterial suborder Pseudonocardineae. The 8,248,144 bp long single replicon genome with its 7100 ...

UK PubMed Central (United Kingdom)

Analysis of the pulsed-field gel electrophoretic profiles of 82 pneumococcal isolates with reduced susceptibility to ciprofloxacin (RSC) and of 90 co-occurring susceptible isolates indicates a considerable...Full Text Available

UK PubMed Central (United Kingdom)

A thermophilic methanogen was isolated from enrichment cultures originally inoculated with sludge from an anaerobic kelp digester (55°C). This isolate exhibited a temperature optimum of 55 to...Full Text Available

UK PubMed Central (United Kingdom)

The level of total extracellular neuraminidase produced by 74 clinical isolates of group B streptococci isolated from diseased or asymptomatically colonized infants was assayed. Extracellular neuraminidase...Full Text Available

Science.gov (United States)

An almost linear optimization problem of importance in vibration isolation has been identified and algorithms were developed to minimize the forced vibrational response of structural systems. The constraints can be either displacements of accelerations. T...

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... biologically important compounds is sharply lowered with passage of the solutions to the cell an organ, where their sensitivity ...

CERN Document Server

Designing the Smart Organization

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The paper describes the production of highly enriched isotopes of uranium, plutonium, americium and curium by electromagnetic separation for scientific and applied researches in physics, chemistry, geology, medicine, biology and other fields. Using the equipment described, the isotopes are produced in quantities sufficient to set up nuclear physical experiments, to produce nuclear reference materials and standard sources for calibration of radiometrical and mass spectrometrical equipment, in radionuclide metrology, etc. For the following isotopes the indicated degrees of isotopic enrichment were achieved: "2"3"3U-99.97%; "2"3"5U-99.97%; "2"3"6U-98.0%; "2"3"8U-99.997%; "2"3"8Pu-99.6%; "2"3"9Pu-99.9977%; "2"4"0Pu-99.9-100%; "2"4"1Pu-96.998%; "2"4"2Pu-97.8-99.96%; "2"4"4Pu-96.7%; "2"4"1Am-99.6%; "2"4"2"mAm-73.6%; "2"4"3Am-99.2-99.94%; "2"4"3Cm-99.99%; "2"4"5Cm-99.998%; "2"4"6Cm-99.8%; "2"4"7Cm-90%, "2"4"8Cm-97%. Methods for preparing layers of highly enriched isotopes on various ...

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PMID:20576129

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Pectin rich cold stored spoiled fruits, vegetables and cold soils were screened and different pectinolytic isolates were obtained by enrichment culturing and ruthenium red plate assay. Among the primary isolates 10-15% were yeast isolates. Six isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase (PGU) production at room temperature (25 degrees C) and at 5 degrees C. One isolate identified as Saccharomyces sp. with highest polygalacturonase activity at 5 degrees C was used for enzyme production using raw fruit pectins as substrates. The isolate was identified by preliminary cultural, morphological and sugar fermentation tests. PGU production was high in raw pectin substrates like orange peel (21 U/ml), apple peel (20 U/ml ), mango peel (19 U/ml), ...

International Nuclear Information System (INIS)

Studies have shown that CCl_4 administration to rats inhibits endoplasmic reticulum calcium pump activity and reduces the amount of calcium associated with subsequently isolated microsomal subcellular fractions. This report confirms that exposure of isolated hepatocytes to CCl_4 rapidly produces these effects in isolated parenchymal cells and demonstrates that when isolated hepacytes are exposed to CCl_4 calcium is rapidly released from cells. This release can be detected with a calcium ion-selective electrode when cells are incubated in a medium with low extracellular calcium. Calcium released from an intracellular pool(s) may initiate hepatotoxic changes in liver. (author).

Energy Technology Data Exchange (ETDEWEB)

This paper addresses one source of degradation in OPV devices: the metal/organic interface. The basic approach was to study the completed device stability vs. the stability of the organic film itself as shown in subsequent devices fabricated from the films.

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CERN Document Server

Role of organic acids in promoting colloidal transport of mercury from mine tailings

UK PubMed Central (United Kingdom)

BackgroundWe examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals.MethodsSeventeen...Full Text Available

UK PubMed Central (United Kingdom)

The spirochete that causes tick-borne relapsing fever, Borrelia hermsii, was isolated in pure culture during 1995 and 1996 from three acutely ill human patients infected in southern...Full Text Available

UK PubMed Central (United Kingdom)

1. l(+)-Methionine sulphoxide has been isolated for the first time from a natural source, the blowfly Phormia regina. 2. The natural compound is shown to be identical with...Full Text Available

UK PubMed Central (United Kingdom)

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein...Full Text Available

Science.gov (United States)

This paper addresses the major issues beginning teachers face in their first postings to rural areas in Australia. The issues go beyond pedagogy. Beginning teachers need to understand social factors affecting rural communities so they can link lessons to the realities experienced by students. Geographic isolation affects the provision of education in terms of time taken to travel, cost, terrain, and technology. Rural communities have groups that are socially isolated from each other and from the staff in schools. In order to teach effectively, teachers need to be aware of the social context of the community in which they live and work. Teachers in rural areas suffer from professional isolation and often confront teaching situations for which they have had little formal preparation. Communities in remote places often move quickly to adopt technological means to overcome their isolation. Teachers in ...

UK PubMed Central (United Kingdom)

AIM: To isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome.METHODS: SW1116 cells were isolated and cultured with...Full Text Available

UK PubMed Central (United Kingdom)

Pfister, Robert M. (Lamont Geological Observatory, Palisades, N.Y.), and Paul R. Burkholder. Numerical taxonomy of some bacteria isolated from Antarctic and tropical seawaters. J....Full Text Available

UK PubMed Central (United Kingdom)

A 42% (70 of 167 isolates) incidence of resistance to 20 micrograms of trimethoprim per ml was found among clinical isolates of coagulase-negative staphylococci from two hospitals. A specific trimethoprim...Full Text Available

UK PubMed Central (United Kingdom)

Isolates of the most commonly observed salmonella serovars in Norwegian fish feed factories from 1998 to 2000 (Salmonella enterica serovar Agona, S. enterica...Full Text Available

UK PubMed Central (United Kingdom)

A lentivirus was isolated from 2 goats in Mexico that were seropositive to caprine arthritis encephalitis virus (CAEV) by the agar gel immunodiffusion (AGID) test. The lentivirus was identified as CAEV...Full Text Available

UK PubMed Central (United Kingdom)

Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing...Full Text Available

UK PubMed Central (United Kingdom)

Two gram-negative, motile bacteria isolated from deep subsurface sediments mineralized the nitrogen-containing polyaromatic hydrocarbon quinoline under aerobic conditions and transformed quinoline to...Full Text Available

UK PubMed Central (United Kingdom)

A Sepharose-coupled 19S human thyroglobulin has been used as an immunoadsorbent to isolate anti-thyroglobulin autoantibodies and to evaluate the antigen-antibody interactions. With the system proposed...Full Text Available

UK PubMed Central (United Kingdom)

The effect of growth phase on expression of virulence-associated factors was studied by Northern hybridization in an M1T1 clinical isolate of Streptococcus pyogenes. Expression of M...Full Text Available

UK PubMed Central (United Kingdom)

Among 149 extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae isolates collected from patients in southeast Austria from 1998 to 2004, 38 Escherichia coli...Full Text Available

UK PubMed Central (United Kingdom)

Haemophilus parasuis causes polyserositis in swine. Fifteen serovars have been characterized by immunodiffusion test, but many field strains are not typeable. Isolates (n...Full Text Available

UK PubMed Central (United Kingdom)

A strain from human diarrhea originally identified as Campylobacter mucosalis (NCTC 12408) was examined by using 64 phenotypic characters. The similarity of this strain to 297 isolates of Campylobacter,...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundSocial isolation affects a significant proportion of older people and is associated with poor health outcomes. The current evidence base regarding the effectiveness of...Full Text Available

UK PubMed Central (United Kingdom)

Fifty-two isolates of Fusarium species were obtained from soybean seeds from various parts of Korea and identified as Fusarium oxysporum, F. moniliforme,...Full Text Available

International Nuclear Information System (INIS)

Portuguese 2009 1 p. Brazil Magalhaes, Cassia Goncalves Silva, Gracia

KoreaScience (Korea)

Full Text Available

International Nuclear Information System (INIS)

Portuguese 2004 [1 p.] Brazil Silva, Silvia Ribeiro de Souza e Silva,

KoreaScience (Korea)

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KoreaScience (Korea)

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KoreaScience (Korea)

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International Nuclear Information System (INIS)

Portuguese (Jul 1981). Brazil Sousa, JR de Souza, E. de . Minas Gerais Univ.,

Science.gov (United States)

Acetylcholine-rich synaptic vesicles were isolated from myenteric plexus-longitudinal muscle strips derived from the guinea pig ileum by the method of Dowe, Kilbinger, and Whittaker [J. Neurochem. 35, 993-1003 (1980)] using either unstimulated preparations or preparations field-stimulated at 1 Hz for 10 min using pulses of 1 ms duration and 10 V . cm-1 intensity. The organ bath contained either tetradeuterated (d4) choline (50 microM) or [3H]acetate (2 muCi . ml-1); d4 acetylcholine was measured by gas chromatography-mass spectrometry. As with Torpedo electromotor cholinergic vesicle preparations made under similar conditions the distribution of newly synthesized (d4 or [3H]) acetylcholine in the zonal gradient from stimulated preparations was not identical with that of endogenous (d0, [1H]) acetylcholine, but corresponded to a subpopulation of denser vesicles (equivalent to the VP2 fraction from Torpedo) that had preferentially taken up newly ...

International Nuclear Information System (INIS)

The patients studied were referred for routine abscess localization with indium-111 labelled autologous leukocytes. One series (n=15) was studied at 1 day after reinjection of labelled cells and a second series (n=9) was studied between 4 hours and 4 days after reinjection of labelled cells. The leukocytes were isolated by a gravity sedimentation method and labelled with In-111 oxinate according to Roevekamp and co-workers. The labelled cell suspension administered to the patient contained between 11 and 15 MegaBq (300-400 microCi). The indium-111 concentration in the liver and spleen was quantitated using a scintillation camera and an on-line computer system. Phantom studies were carried out to obtain conversion factors from geometric mean counts to MegaBq of activity for both liver and spleen. Once determined, the amount of activity in liver and spleen was corrected for physical decay of In-111 and expressed as a percentage of the administered activity. ...

Energy Technology Data Exchange (ETDEWEB)

Anionic complexes of transition metals were stabilized in aqueous solutions containing high concentrations of various short-chain quaternary ammonium salts. Compounds with longer paraffin chains were effective in much less concentrated solution. Complex ions were detected spectrophotometrically. FeCl/sub 4//sup -/, which is usually formed in concentrated HCl, was the predominant Fe(III) complex in 30 m choline chloride containing only 0.12 M HCl. A yellow transitory Tc(VII) chloro-addition intermediate, formed in the reduction of TcO/sub 4//sup -/ by concentrated HCl, was stabilized when the solution also contained 25 m choline chloride. Its spectrum, as well as the isolation of an already known Tc(VII) bipyridyl complex, is reported. Concentrated organic electrolytes also stabilized Tc(V) oxide halides against disproportionation and Tc(IV) hexahalides against hydrolysis. Halochromates of Cr(VI) were formed and stabilized in dilute acid ...

Energy Technology Data Exchange (ETDEWEB)

The emission isolation flux chamber (EIFC) methodology was applied to Superfund investigations at the Lawrence Livermore National Laboratory Site 300 to determine if on-site workers were exposed to VOCs volatilizing from the subsurface and what, if any, health risks could be attributed to the inhalation of the VOCs volatilizing from the subsurface. During July and August of 1996, twenty, eighteen, and twenty six VOC soil vapor flux samples were collected in the Building 830, 832, and 854 areas, respectively using EIFCS. The VOC concentrations in the vapor samples were used to calculate soil flux rates which were used as input into an air dispersion model to calculate ambient air exposure-point concentrations. The exposure-point concentrations were compared to EPA Region IX Preliminary Remediation Goals (PRGs). Buildings 830 and 832 exposure-point concentrations were less then the PRGs therefore no cancer risks were calculated. The cancer risks for Building 854 ...

UK PubMed Central (United Kingdom)

The extremely thermophilic anaerobic archaeon strain B1001 was isolated from a hot-spring environment in Japan. The cells were irregular cocci, 0.5 to 1.0 μm in diameter. The new isolate grew...Full Text Available

UK PubMed Central (United Kingdom)

Fifteen strains of CDC group 1 coryneform and biochemically similar bacteria were isolated from clinical specimens. Of the 15 strains isolated, 11 were derived from abscesses and purulent lesions, mostly...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

In tomato crop, the induction of resistance emerges as an important alternative for achieving the reduction of chemicals in disease control. This study aimed to evaluate the ability of 28 Trichoderma isolates to promote the growth of tomato seedlings and to induce systemic resistance (ISR) against Xanthomonas euvesicatoria and Alternaria solani, the causal agents of bacterial spot and early blight, respectively. Twelve isolates promoted the increase of plant dry matter mass (DMM) above 100%, showing the great potential of these strains. All isolates were able to colonize the root system of tomato plants. The plant growth-promoting isolates were further evaluated for potential elicitation of ISR. Treatment of the soil with all Trichoderma isolates provided protection in tomato plants from 2...

International Nuclear Information System (INIS)

Records of 154 patients with Ewing's sarcoma treated at the National Cancer Institute were reviewed to assess the incidence and risk of developing isolated central nervous system (CNS) Ewing's sarcoma. Sixty-two of the 154 patients had received CNS irradiation and intrathecal (i.t.) methotrexate as part of their initial therapy to prevent the occurrence of isolated CNS Ewing's sarcoma. The risk of developing isolate CNS Ewing's sarcoma was greatest within the first two years after diagnosis and was approximately 10%. The overall risk of CNS recurrence in the group of patients receiving DNS treatment was similar to the group receiving no therapy directed to the CNS. The occurrence of isolated CNS involvement was not prevented by the use of CNS irradiation and i.t. methotrexate. Because of a lack of efficacy to the CNS irradiation regimen, current treatment regimens do not include therapy directed to CNS.

Energy Technology Data Exchange (ETDEWEB)

The concentration of some organic acids in the atmosphere was determined using a wet effluent diffusion denuder-aerosol collector coupled with Ion Chromatography. Three organic acids and four inorganic anions were identified and quantified in the air sample, taken from the backyard of PSI. (author)

Wastenet

... Do you certify Halal or Kosher meat? What fish feed do you allow? What levels of polychlorinated biphenyls (PCBs) have been found in organic fish? How can I be sure that organic products from abroad are really organic? Do you prosecute businesses that break the rules? Does the Soil Association carry out any routine testing of food? Do you test for GM material? Why do you allow additives in organic food? Is organic food fortified with vitamins and minerals? Why choose organic during pregnancy?...

Science.gov (United States)

... TITLE (Include Security Classification) Sorption of Selected Volatile Organic Constituents of Jet Fuels and Solvents on Natural Sorbents from Gas ...

CERN Document Server

Preparation of covalently modified organic-inorganic composite nanoparticles and their interfacial electron transfer researches

Science.gov (United States)

FT-IR spectroscopic studies of major organic matter in carbonaceous chondrites using microscopic technique and comparison with terrestrial kerogen. ...

International Nuclear Information System (INIS)

This paper presents the analytical results of alcohols, esters and organic acids in sweet potato wine irradiated by #gamma#-rays. (author).

Science.gov (United States)

years I chaired a national committee drafting recommended standards for organic livestock production for the Organic Trade Association and had the opportunity to work with...

International Nuclear Information System (INIS)

During spring and autumn, the total number of amoebae and the number of acanthamoeba species able to grow at 37 degrees C were determined in six thermally polluted factory discharges and the surrounding surface waters. The isolated Acanthamoeba strains were studied for growth in axenic medium, cytopathic effect in Vito cell cultures, and virulence in mice. Although more amoebae were isolated in autumn, the number of Acanthamoeba species was lower than in spring, when the percent of pathogenic strains among the isolates was highest. Higher concentrations of amoebae were found in warm discharges, and more virulent strains occurred in thermal discharges than in surface waters.

DEFF Research Database (Denmark)

Objectives: The purpose of this work was to study the genetic determinants responsible for extended-spectrum beta-lactamase (ESBL) resistance of Salmonella isolated from Dutch poultry, poultry meat and hospitalized humans. Methods: Thirty-four ESBL-resistant Salmonella isolates from The Netherlands were tested towards 21 antimicrobial agents. PCR and sequencing were used to determine the underlying genetic determinants responsible for the ESBL phenotypes. The transferability of the ESBL phenotypes was tested by conjugation to a susceptible Salmonella enterica serovar Dublin and plasmid purification, restriction fragment length polymorphism (RFLP) and pulsed-field gel electrophoresis (PFGE) were employed to further characterize a subset of the isolates. Results: A great genetic diversity was seen among the isolates. The bla(TEM-52) gene was most predominant and was found among Salmonella enterica ...

British Library Electronic Table of Contents (United Kingdom)

Wound dressings impregnated with silver have a role to play in aiding to reduce both the dressing and wound microbial bioburden. It is therefore imperative that antimicrobial wound dressings have efficacy on a broad range of clinical significant microorganisms. Accordingly, this study aimed to determine the antimicrobial efficacy of a silver alginate dressing against 115 wound isolates that had been isolated routinely from patients at West Virginia University Hospital. Standardised corrected zones of inhibition (CZOIs) were performed on all clinical isolates. It was found that the silver alginate dressing was able to inhibit the growth of all microorganisms tested. In particular, the silver alginate dressing inhibited the growth of Candida albicans and yeasts with CZOI of 3-115 mm. All met...

Science.gov (United States)

... video recorded in an isolated booth under a Panasonic WV-CP470 video camera located in an environmentally ... ...

International Nuclear Information System (INIS)

More than sixty base-isolated buildings have been built in Japan. A number of base-isolation systems were considered in our research, which was intended to establish the effectiveness of base-isolation systems. We conducted research on silicone rubber bearings. Generally, silicone rubber is durable and its characteristics are not dependent on the temperature within the relevant design range. The first part of the report covers material and elements testing. After the bearings were installed in the building, we performed forced vibration tests in both the horizontal and vertical directions. These test results form the next section. After several experiments, we carried out earthquake observations. We report on the effectiveness of the system in reducing response acceleration during a small displacement. This system was installed in the building in March 1992

Science.gov (United States)

... B. P. Rosen. An arsenic metallochaperone for an arsenic detoxification pump. Proc. Natl. Acad. Sci. U. S. A 103( ... ...

Science.gov (United States)

... problems by isolating them. 4 LAN Segmentation, http://netcert.tripod.com/ ccna/internetworking/lanseg.html, Jan 2005 Destination ...

DEFF Research Database (Denmark)

Udgivelsesdato: 2009-Dec

UK PubMed Central (United Kingdom)

BackgroundIt is well established that Legionella pneumophila is a waterborne pathogen; by contrast, the mode of Helicobacter pylori transmission...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

SummaryBackgroundCandida albicans is one of the most important etiologic agents causing superficial and deep fungal infections. For prevention of candidiasis, it is important to develop a rapid system that discriminates C. albicans at the strain level.ObjectiveTo develop a system that can identify C. albicans at the strain level.MethodsGenomic DNAs were purified from 179 clinical isolates of C. albicans, and were used as templates for PCR amplification of 25S rDNA and ALT repeats in repetitive sequences (RPSs). PCR products generated from ALT repeats were digested with EcoRI and/or ClaI in order to study the relationships between restriction profiles and amplification profiles.ResultsOne hundred and seventy nine clinical isolates were grouped into genotypes A (92 isolates), B (38 isolates)...

UK PubMed Central (United Kingdom)

A group of patients is described who developed the clinical features of foveomacular retinitis. No causative factors were isolated, and all patients strongly denied any type of sun gazing. It is possible...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

This report describes a simple seismic analysis model of the KALIMER-600 sodium cooled fast reactor and its application to the seismic time history analysis. To develop the simple seismic analysis model, the detailed 3-D finite element analyses for main components, IHTS piping system, and reactor building were carried out to verify the dynamic characteristics of each part of simple seismic analysis models. By using the developed simple model, the seismic time history analyses for both cases of a seismic isolation and non-isolation design of KALIMER-600 were performed. From the comparison of the calculated floor response spectrum, it is verified that the seismically isolated KALIMER-600 reactor building shows a great performance of a seismic isolation and assures a seismic integrity.

UK PubMed Central (United Kingdom)

Numerous bacteria from a salt-marsh environment are capable of degrading malathion, an organophosphate insecticide, when supplied with additional nutrients as energy and carbon sources. Seven isolates...Full Text Available

Science.gov (United States)

... Except for isolated radiation accidents over the ensuing years, little practical experience has been gained in the treatment of thermal injuries ...

UK PubMed Central (United Kingdom)

This paper describes attempts to isolate and characterize glycosaminoglycan (GAG)-binding molecules on the surface of lymphocytes and lymphoma cell lines and relate their expression to splenic and lymph...Full Text Available

Science.gov (United States)

... being designed for projected Minotaur IV launch load environments, with design objectives of light weight, integrated vibration isolation, low shock ...

Science.gov (United States)

... Confirm isolates that appear as typical E. coli on L-EMB and are X-gal (+), MUG (+) or (-) and ... Hill, WE, KC Jinneman, PA Trost, JL Bryant, J. Bond and MM Wekell ...

Science.gov (United States)

Staphylococcus aureus isolates and 2 USA300 ATCC type strains for susceptibility to bacitracin and neomycin contained in over-the-counter antibacterial ointments. Resistance to...

Science.gov (United States)

Binary Star Evolution. The evolution of close binary stars can be very different from that of wide binaries of isolated stars. If the stars are close enough ...

Science.gov (United States)

Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

International Nuclear Information System (INIS)

... organizations irradiation radiation doses radiation effects RADIATIONS.

KoreaScience (Korea)

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Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

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...Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain Assemblages of plants and invertebrate animals of tumulus ...organic mound) springs of the Swan Coastal Plain [Skip navigation links] About us | Contact us | Publications |...ecological communities Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain Advice to the Minister for the ...of the 'Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain' community sufficient to distinguish it from ...

International Nuclear Information System (INIS)

As the Operating and Support staffs of the FFTF organization have gained experience, the plant reliability and capacity factors have shown a steadily improving trend. The plant capacity factor for Cycle 4 was 99.5%. It is the purpose of this report to describe the evolution of the maintenance organization at the FFTF site from a general support organization to a technically proficient organization playing a major role in planning and performance of plant maintenance evolutions.

KoreaScience (Korea)

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Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

DEFF Research Database (Denmark)

Udgivelsesdato: 2001-Mar

Science.gov (United States)

Breath Tests; Human Volunteers; Pilot Study

KoreaScience (Korea)

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Energy Technology Data Exchange (ETDEWEB)

One of the most challenging topics in the area of organic electronic devices is the growth of transparent electrodes onto flexible polymeric substrates that will be characterized by enhanced conductivity in combination with high optical transparency. An essential aspect for these materials is their synthesis and/or microstructure which define the transparency, the stability and the interfacial chemistry which in turn determine the performance and stability of the organic electronic devices, such as organic light emitting diodes, organic photovoltaics, etc. In this work, we will discuss the latest advances in the growth of organic (e.g. PEDOT:PSS) and inorganic (e.g. zinc oxide-ZnO, indium tin oxide-ITO) conductive materials and their deposition onto flexible polymeric substrates. We will compare the optical, structural, nano-mechanical and nano-topographical properties of the ...

Energy Technology Data Exchange (ETDEWEB)

In order to calculate the doses received by the organs of 530 children treated by radiation for cancer between 1945 and 1969 at the G. Roussy Institute, we have developed a computer program for organ location calculation. To calculate the location of each child's organs of interest at the time of the treatment, only two parameters are necessary; sex and height or sex and age when the height at the time of the treatment is unknown. The algorithm is based on the metric studies of growth known as auxology. Each organ is located by one point representing its center. The model has been checked on 100 healthy children.

UK PubMed Central (United Kingdom)

Among 235 extended-spectrum β-lactamase-producing Klebsiella pneumoniae (ESBL) isolates collected from a nationwide surveillance performed in Taiwan, 102 (43.4%) were...Full Text Available

UK PubMed Central (United Kingdom)

Isolated fifth internodes of Coleus blumei Benth. were supplied with ³H-IAA at their apical ends. Microradioautography, using thin sections (0.25-0.5 microns) of Epon embedded...Full Text Available

UK PubMed Central (United Kingdom)

Seventeen clinical isolates of Staphylococcus aureus (from the United States and Europe) selected for low (borderline)-level methicillin resistance (MIC of methicillin, 2 to 4 micrograms/ml; MIC of...Full Text Available

UK PubMed Central (United Kingdom)

Among 177 extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates collected from 11 Slovenian hospitals in 2005 and 2006, 60 (34%), from eight hospitals,...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

In Chapter 2 the evaluation methods of input ground motions for seismic design are summarized and especially the empirical and/or stochastic Green's function method is introduced in order to calculate the broadband strong motions based on the physically reliable fault model. Then the evaluation procedure is applied to the Ulsan fault system near which Wolsung nuclear power plant is located. In chapter3, the PSHA case studies for Wolsung NPP site are performed, in which multi attenuation equations of earthquake motions are adopted. Moreover, the results of seismic hazard evaluation for eight sites in Korea are shown. In addition, the contents of two literatures on seismic hazard evaluation are introduced. In chapter 4, isolation devices for emergency diesel generator have been studied. The models for friction-pendulum system, high damping rubber bearing and natural rubber bearing are developed and implemented in the TDAP-III. Then, the rubber bearings as ...

UK PubMed Central (United Kingdom)

Two cDNA libraries corresponding to polyA+ RNA from human adult skeletal muscle have been constructed by cloning in the PstI site of pBR322. Skeletal alpha actin cDNA clones have been isolated and characterized....Full Text Available

UK PubMed Central (United Kingdom)

We previously showed that very thin filamentous bacteria affiliated with the division green non-sulfur bacteria were abundant in the outermost layer of thermophilic methanogenic sludge granules fed...Full Text Available

UK PubMed Central (United Kingdom)

Two clinical strains of Escherichia coli (2138) and Enterobacter cloacae (7506) isolated from the same patient in France and showing resistance to extended-spectrum...Full Text Available

UK PubMed Central (United Kingdom)

We report the emergence of Salmonella enterica isolates of serotype Concord (and its monophasic variant 6,7:l,v:-) producing the extended-spectrum β-lactamases (ESBLs) SHV-12...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundA high rate of resistance to 3^rd generation cephalosporins among Enterobacteriaceae isolates from Egypt has been previously reported. This study aims to characterize...Full Text Available

UK PubMed Central (United Kingdom)

Salmonella enterica subsp. enterica serovar Typhimurium was isolated from diarrheic piglets in 2 periods, 2000–2001 (n = 25) and 2005–2006...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Specific {sup 32}P-labeled DNA probes were prepared and used to evaluate the distribution of tetracycline resistance determinants carried by gram-negative enteric bacteria isolated from pigs in 3 swine herds with different histories of antibiotic exposure. Plasmid DNA, ranging in size from 2.1 to 186 Kb, was observed in over 84% of 114 isolates studied. Two of 78 tetracycline resistant strains did not harbor plasmids. The DNA probes were isolated from plasmids pSL18, pRT29/Tn10, pBR322 and pSL106, respectively, and they represented class A, B, C and D tetracycline resistance determinants. Hybridization conditions using 0.5X SSPE at 65{degrees}C minimize cross-hybridization between the different class of tetracycline resistance genes. Cross-hybridization between class A and class C determinants could be distinguished by simultaneous comparison of the intensity of their hybridization signals. Plasmids from over 44% of the ...

British Library Electronic Table of Contents (United Kingdom)

Abstract:- This study was undertaken to investigate the antimicrobial effect of organic acids against-Escherichia coli-O157:H7,-Salmonella-Typhimurium, and-Listeria monocytogenes-on whole red organic apples and lettuce. Several studies have been conducted to evaluate organic acids as sanitizers. However, no studies have compared antimicrobial effects of various organic acids on organic fresh produce, including evaluation of color changes of produce. Apples and lettuce were inoculated with a cocktail of 3 strains each of 3 foodborne pathogens provided above and treated with 1% and 2% organic acids (propionic, acetic, lactic, malic, and citric acid) for 0, 0.5, 1, 5, and 10 min. With increasing treatment time and acid concentration, organic acid treatments showed significant reduction compar...

British Library Electronic Table of Contents (United Kingdom)

The erosion of rocks rich in organic matter typically leads to the complete mineralization of the organic material. However, in some cases, it is re-deposited to become a part of sediments once more. This process should be considered to be a part of global carbon cycle, possibly much more significant than assumed to-date. The research presented here aims to characterize re-worked organic matter occurring in post-glacial sediments of southern part of Poland, in the Oder river valley (the Racib?rz town region, Miocene, Pleistocene and Holocene age). Organic substances extracted from the sediments originated from organic matter that had resided in rocks eroded by glaciers. Sediments were sampled in two boreholes which sediments were correlated. Sediments were extracted and extracts analyzed w...

British Library Electronic Table of Contents (United Kingdom)

Biological control agents can be more effective if their populations are genetically diverse, particularly when the target invasive plant comprises a range of genotypes with different susceptibilities and occurs across various microclimates. We report on the use of an efficient approach to find, in the native range, diverse isolates of a rust fungus for biological control. An outdoor trap garden containing various clones of invasive European blackberry (Rubus fruticosus agg.) collected in Australia, each with a different DNA phenotype, was established in France. Within 4?weeks of establishment, the leaf-rust fungus Phragmidium violaceum was recovered from all clones in the garden. Molecular analyses of eight recovered and purified isolates of the fungus from the garden revealed that they w...

British Library Electronic Table of Contents (United Kingdom)

Internet is providing essential communication between an infinite number of people and is being increasingly used as a tool for commerce. At the same time, security is becoming a tremendously important issue to deal with. Different network security solutions exist and contribute to enhanced security. From these solutions, Intrusion detection systems (IDS) have become one of the most common countermeasures for monitoring safety in computer systems and networks. The purpose of IDSs is distinguishing between intruders and normal users. However, IDSs report a massive number of isolated alerts. These isolated alerts represent low-level security-related events. Many of these isolated alerts are logically involved in a single multi-stage intrusion incident and a security officer often wants to an...

British Library Electronic Table of Contents (United Kingdom)

Isolation of functional and intact mitochondria from solid tissue is crucial for studies that focus on the elucidation of normal mitochondrial physiology and/or mitochondrial dysfunction in conditions such as aging, diabetes, and cancer. There is growing recognition of the importance of mitochondria both as targets for drug development and as off-target mediators of drug side effects. Unfortunately, mitochondrial isolation from tissue is generally carried out using homogenizer-based methods that require extensive operator experience to obtain reproducible high-quality preparations. These methods limit dissemination, impede scale-up, and contribute to difficulties in reproducing experimental results over time and across laboratories. Here we describe semiautomated methods to disrupt tissue ...

Science.gov (United States)

30 species belonging to 18 genera were isolated from floor dust of 30 homes in Riyadh. Out of them 16 species and 10 genera were isolated from dust of air conditioners of the same homes. The most common genera in floor dust were Aspergillus, Penicillium and Cladosporium. Aspergillus repens, A. amstelodami, A. versicolor, A. fumigatus, Penicillium purpurogenum, P. crustosum, Cladosporium cladosporoides and C. herbarum were frequently isolated. The most abundant genera in air conditioner dust were Aspergillus and Penicillium. Aspergillus fumigatus, A. ochraceus, A. terreus, Penicillium oxalicium and P. crustosum were most frequent species. PMID:2330766

British Library Electronic Table of Contents (United Kingdom)

Extraction of 25 L fermentation broth of the newly isolated Streptomyces sp. strain TN58 and various separation and purification steps led to the isolation of five bioactive metabolites, namely brevianamide F (C1), reported from a streptomycete for the first time, N?-acetyltryptamine (C2), thiazolidomycin (C3), and two rhamnopyranosides (C4 and C5). These two rhamnopyranosides were produced directly, without precursor addition. The chemical structure of these five active compounds was established on the basis of 1H, 13C/APT and 2D NMR spectra, ESI and EI-MS data, and by comparison with data from the literature. According to the biological studies, we show in this work that the compounds C1, C2, C4 and C5 possess antimicrobial activities.

Energy Technology Data Exchange (ETDEWEB)

A dielectric-wall linear accelerator is improved by a high-voltage, fast rise-time switch that includes a pair of electrodes between which are laminated alternating layers of isolated conductors and insulators. A high voltage is placed between the electrodes sufficient to stress the voltage breakdown of the insulator on command. A light trigger, such as a laser, is focused along at least one line along the edge surface of the laminated alternating layers of isolated conductors and insulators extending between the electrodes. The laser is energized to initiate a surface breakdown by a fluence of photons, thus causing the electrical switch to close very promptly. Such insulators and lasers are incorporated in a dielectric wall linear accelerator with Blumlein modules, and phasing is controlled by adjusting the length of fiber optic cables that carry the laser light to the insulator surface.

Science.gov (United States)

A six-axis active vibration isolation system (AVIS) is designed by using the direct driven guide and ball contact mechanisms in order to have no cross-coupling between actuators. The point contact configuration gives an advantage of having an easy assembly of eight voice coil actuators to an upper and a base plate. A voice coil actuator is used since it can provide a large displacement and sufficient bandwidth required for vibration control. The AVIS is controlled considering the effect of flexible vibration mode in the upper plate and velocity sensor dynamics. A loop shaping technique and phase margin condition are applied to design a vibration controller. The performances of the AVIS are investigated in the frequency domain and finally validated by comparing with the passive isolation system. The scanning profiles of the specimen are compared together by using the atomic force microscope. The robustness of the AVIS is verified by showing the ...

British Library Electronic Table of Contents (United Kingdom)

Functional properties of commercial and membrane processed pea protein isolates (PPI) prepared from yellow peas were investigated. Four protein isolates were prepared from yellow pea flour using water and KCl extractions at 25^oC followed by ultrafiltration and diafiltration (UF and DF) at pHs of 7.5 and 7.5 or 6 respectively. Following assessment of compositional attributes; solubility, foaming, flow and dynamic rheology, emulsification ability and heat-induced textural and rheological properties of prepared PPIs and a commercially available PPI were tested and compared. Membrane purification of proteins resulted in 28% to 68% reduction in phytic acid and enhanced, comparatively, the tested functional properties. Solubility of membrane processed PPIs, at all tested pHs, was superior and t...

British Library Electronic Table of Contents (United Kingdom)

This study concerns the thermotolerance of spores of mesophilic fungi isolated from a tropical semi-arid habitat subject to dry season fire in the Western Ghats, southern India. Among 25 species of Ascomycota isolated from leaf litter, nine were able to grow after incubation in a drying oven for 2h at 100degreeC; the spores of two of these species survived 2h incubation at 110degreeC, and one survived exposure to 115degreeC for 2h. The range of thermotolerance among mesophilic fungi isolated from the leaf litter was surprising: filamentous fungi from other habitats, including species that colonize scorched vegetation after fires and thermophilic forms occurring in self-heating plant composts, cannot survive even brief exposure to such high temperatures. It is possible that the exceptional ...

Wastenet

...qualified and who were under consideration at the time, USDA Secretary Tom Vilsack chose an animal husbandry specialist employed by one of the largest organic livestock product marketers in the country. While this appointee had grown up on a conventional farm, her immediate occupation is not that of ...

UK PubMed Central (United Kingdom)

To study the topographical organization of mu and beta band event-related desynchronization (ERD) associated with voluntary hand and foot movements, we used magnetoencephalographic (MEG) recordings...Full Text Available

UK PubMed Central (United Kingdom)

Microbes in nature frequently function as members of complex multitaxon communities, but the structural organization of these communities at the micrometer level is poorly understood because of limitations...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The subfornical organ is a major receptor area for one of the principal stimuli of thirst, the octapeptide, angiotensin II. In conscious water-sated rats, the authors examined the effects of intravenous infusion of angiotensin II on the rate of glucose utilization in the subfornical organ and in structures anatomically and functionally connected with it. Angiotensin II produced pressor and drinking responses and increased glucose utilization selectively in the subfornical organ and pituitary neural lobe and in no other brain structure. Treatment with the angiotensin II antagonist, sar1-leu8-angiotensin II, before intravenous administration of angiotensin II prevented metabolic stimulation of the subfornical organ and neural lobe. Captopril, an inhibitor of angiotensin-converting enzyme, reduced subfornical organ glucose metabolism to a level similar to that found in control animals. ...

Science.gov (United States)

Overviews, publications, and new directions are presented for the following research topics: geminate charge pairs in hexane, dipoles in nonpolar and polar organic liquids, organic donor-acceptor interfaces, and charge-transfer states (phenanthrene/PMDA).

UK PubMed Central (United Kingdom)

An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble)...Full Text Available

UK PubMed Central (United Kingdom)

Large quantity of reliable protein interaction data are available for model organisms in public depositories (e.g., MINT, DIP, HPRD, INTERACT). Most data correspond to experiments with the proteins...Full Text Available

Science.gov (United States)

The corrosion behavior of Ta, Ti, and Zr in inorganic acids, bases, chlorides and miscellaneous salts, waters and gases, and organic acids and miscellaneous organic chemicals is summarized. (W.L.H)

Energy Technology Data Exchange (ETDEWEB)

This book provides differential diagnostic possibilities of specific scan abnormalities in one volume for easy reference. This book is organized by organ systems and covers the central nervous system, the endocrine, skeletal, pulmonary, gastrointestinal, genitourinary, and cardiovascular systems. The gamuts are designed to teach differential diagnosis and to assist in the actual interpretation of patient slides.

UK PubMed Central (United Kingdom)

BackgroundSelenium is a trace element performing important biological functions in many organisms including humans. It usually affects organisms in a strictly dosage-dependent manner...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

This report describes the work performed during FY 1995 by Pacific Northwest Laboratory in developing and optimizing analysis techniques for identifying organics present in Hanford waste tanks. The main focus was to provide a means for rapidly obtaining the most useful information concerning the organics present in tank waste, with minimal sample handling and with minimal waste generation. One major focus has been to optimize analytical methods for organic speciation. Select methods, such as atmospheric pressure chemical ionization mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry, were developed to increase the speciation capabilities, while minimizing sample handling. A capillary electrophoresis method was developed to improve separation capabilities while minimizing additional waste generation. In addition, considerable emphasis has been placed on developing a rapid screening tool, based ...

Environmental Research Database

DescriptionChamber studies have shown that biogenic organic compounds, including isoprene, are capable of producing secondary organic aerosol in significant quantities. This is surprising as isoprene and its photo-oxidation products have relatively high vapour pressures. However, it is very important as isoprene has the largest global emissions flux of any biogenic hydrocarbon, most of which is in the tropics. Despite this, secondary organic aerosol has been poorly studied in tropical regions with only [continued...

Science.gov (United States)

... 5. FUNDING NUMBERS ... SEATO SOUTHEAST ASIA TREATY ORGANIZATION TTP TEHRIK-I-TALIBAN PAKISTAN Page 13. xii ...

British Library Electronic Table of Contents (United Kingdom)

This forum showcases the work of a variety of different heritage-based centers, organizations, and projects dedicated to research, education, and preservation of tangible and intangible forms of cultural heritage. The descriptions of these centers demonstrate the diversity of heritage work being done today. The centers and projects described in the forum vary in their contexts, missions, and outcomes. Highlighted in the forum are preservation organizations, university-based heritage centers, and a global collaborative cultural heritage project. Each organization in the forum provides information about their missions and goals, their approaches or methods to heritage work, and a brief description of some of their initiatives.

Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

Energy Technology Data Exchange (ETDEWEB)

Flexible solar cells or luminous foils have become reality with organic electronic systems.

Energy Technology Data Exchange (ETDEWEB)

The purpose of this document is to describe the NIF Project Organization and the roles and responsibilities of the managers charged with executing the Project.

Science.gov (United States)

... outside observers, and updated counseling requirements for the Army's OER, non-commissioned officer efficiency report (NCOER), and civilian ...

Energy Technology Data Exchange (ETDEWEB)

The carbon cycle is an important part of major biogeochemical cycles. Many techniques may be used to characterize carbon amounts and sources in the environment. Here we first review the most popular techniques for the determination of organic and inorganic carbon concentrations. Decarbonatation techniques are also reviewed in details since it is often an important part of organic carbon analysis. The second part of this paper addresses the use of carbon stable isotopes to characterize organic carbon sources and processes in the environment. An overview of general stable isotopes background and terminology is given as well as the most popular analytical techniques.

Science.gov (United States)

No abstract available.

Wastenet

The Commission also agreed on new guidelines for organic livestock production, supporting natural breeding methods and

International Nuclear Information System (INIS)

Zr-Al metallo-organic compounds were thermally decomposed to prepare ZrO_2-Al_2O_3 composite powders. The specific surface area was dependent on the organofunctional groups of the starting organic compounds. The pore size distribution was dependent on the heating rate when the heating temperature was the same. 4 refs.; 4 figs.

Energy Technology Data Exchange (ETDEWEB)

The use of filters of activated-charcoal particles in drinking-water purification plants assists in the elimination of organic micropollutant substances. The authors report on a study which sought to investigate the mechanism of organic matter; they describe the experimental programme, which used different size ranges of mineral-based activated charcoal, present the experimental findings and discuss the results. 16 references.

Energy Technology Data Exchange (ETDEWEB)

Loss of organic matter is one of the main forms of soil degradation in Mediterranean agricultural soils, and external sources of organic matter are required to improve soil properties. the two-phase centrifugation system in the olive-oil extraction industry produces a large amount of olive mill waste sludge (TPOMW) which can be used to add organic C to degraded soils. (Author)

International Nuclear Information System (INIS)

Fe and Zn were determined in various parts of maize (Zea mays) in dependence on quantity of organic substrate EKOFERT as organic fertilizer in soil, using radionuclide X-ray fluorescence analysis. The increase of quantity of organic substrate EKOFERT in soil causes a decrease of heavy metal concentrations in certain parts of the plant. (author). 4 refs., 1 tab.

Environmental Research Database

DescriptionThe objective of this project is to relate the processing quality of wheat to the composition of the developing and mature grain using material grown under a range of nutrient regimes including long term organic and fertilised systems from the Rothamsted Broadbalk experiment, variety trials and organically grown wheat. Preliminary studies have indicated substantial differences in the transcriptome profiles of wheat grown with artificial fertiliser and with organic fertiliser on Broadbalk and tru [continued...

UK PubMed Central (United Kingdom)

Oligonucleotide DNA probes complementary to the hypervariable region of the 16S rRNA of Bacteroides forsythus were tested for their specificity and sensitivity against reference and clinical isolates...Full Text Available

UK PubMed Central (United Kingdom)

Strain F, a recently isolated ruminal bacterium, grew rapidly with glutamate or glutamine as an energy source in the presence but not the absence of Na. Monensin, a Na+/H+ antiporter, completely inhibited...Full Text Available