UK PubMed Central (United Kingdom)

B6C3F1 mice chronically exposed to 3,3′,4,4′-tetrachloroazobenzene (TCAB), a contaminant of dichloroaniline-derived herbicides, developed a number of neoplastic and nonneoplastic...Full Text Available

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Subchronic exposure to pharmacological levels of estrogenic compounds, including 17 beta-estradiol, diethylstilbestrol, and alpha-dienestrol, significantly increased the mortality of B6C3F1 female mice...Full Text Available

International Nuclear Information System (INIS)

Metabolism of benzene is thought to be necessary to produce the toxic effects, including carcinogenicity, associated with benzene exposure. To extrapolate from the results of rodent studies to potential health risks in man, one must know how benzene metabolism is affected by species, dose, dose rate, and repeated versus single exposures. The purpose of our studies was to determine the effect of repeated inhalation exposures on the metabolism of [14C]benzene by rodents. Benzene metabolism was assessed by characterizing and quantitating urinary metabolites, and by quantitating 14C bound to hemoglobin and micronuclei induction. F344/N rats and B6C3F1 mice were exposed, nose-only, to 600 ppm benzene or to air (control) for 6 hr/day, 5 days/week for 3 weeks. On the last day, both benzene-pretreated and control animals were exposed to 600 ppm, ...

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Insulin resistance plays a central role in type 2 diabetes and obesity, which develop as a consequence of genetic and environmental factors. Dietary changes including high fat diet (HFD) feeding promotes...Full Text Available

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Excessive physical activity plays an important role in the progression of anorexia nervosa (AN) by accelerating weight loss during dietary restriction. To search for mechanisms underlying this trait, a panel of mouse chromosome substitution strains derived from C57BL/6J and A/J strains was exposed to a scheduled feeding paradigm and to voluntary running wheel (RW) access. Here, we showed that A/J chromosomes 4, 12 and 13 contribute to the development of a disrupted RW activity in response to daily restricted feeding. This pattern is characterized by intense RW activity during the habitual rest phase and leads to accelerated body weight loss. Regions on mouse chromosomes 4, 12 and 13 display homology with regions on human chromosomes linked with anxiety and obsessionality in AN cohorts. The...

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BackgroundThe scent from receptive female mice functions as a signal, which stimulates male mice to search for potential mating partners. This searching behavior is coupled with...Full Text Available

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Plasma samples were collected from mice infected with Trypanosoma musculi at different times postinfection and administered to naive recipient mice either before or during T. musculi infection. The...Full Text Available

UK PubMed Central (United Kingdom)

Mice are an ideal mammalian model for studying the genetics of aging: considerable resources are available, the generation time is short, and the environment can be easily controlled, an important...Full Text Available

UK PubMed Central (United Kingdom)

The objective of the current research was to examine the association of herd level disease incidence with the return over feed (ROF) (milk income minus feed cost) herd profit index offered through Canwest...Full Text Available

UK PubMed Central (United Kingdom)

The display of proteins such as feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater...Full Text Available

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Abstract Studies of the megamouth shark, one of three planktivorous sharks, can provide information about their evolutionary history. Megamouth shark feeding has never been observed in life animals, but two alternative hypotheses on biomechanics suggest either feeding, i.e., ram feeding or suction feeding. In this study, the second moment of area of the ceratohyal cartilages, which is an indicator of the flexural stiffness of the cartilages, is calculated for 21 species of ram- and suction-feeding sharks using computed tomography. The results indicate that suction-feeding sharks have ceratohyal cartilages with a larger second moment of area than ram-feeding sharks. The result also indicates that the ram-suction index, which is an indicator of relative contribution of ram and suction behavi...

International Nuclear Information System (INIS)

Short communication.

KoreaScience (Korea)

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Purpose: The DBA/2J mice have been used as an animal model for human pigmentary glaucoma. However, these mice develop various degrees of disease symptoms at different ages, making it difficult to detect pathological changes of retinal degeneration at glaucoma onset. The purpose of this study is to develop a non-invasive assay to identify individual mice that develop visual deficits. Materials and Methods: We apply two behavioral tests, a swimming test of visual discrimination and a test of optomotor response, to identify glaucomatous DBA/2J mice. We then examine whether the elevation of intraocular pressure (IOP), the common risk factor for glaucoma, affects visual performances of the DBA/2J mice. We further compare the retinal ganglion cell death, one of the signature glaucoma symptoms, i...

UK PubMed Central (United Kingdom)

We previously reported that global deletion of insulin receptor substrate protein 1 (Irs1) extends lifespan and increases resistance to several age-related pathologies in female mice....Full Text Available

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BackgroundGlucocorticoids down-regulate cytokine synthesis and suppress inflammatory responses. The glucocorticoid receptor (GR) antagonist RU486 may exacerbate the inflammatory...Full Text Available

UK PubMed Central (United Kingdom)

The in vivo antichlamydial activities of sparfloxacin and reference drugs were examined in a experimental model of pneumonia caused by Chlamydia pneumoniae in leukopenic mice; their in vitro activities...Full Text Available

UK PubMed Central (United Kingdom)

Experimental metastases in the brain of mice are infiltrated by microglia, and parabiosis experiments of green fluorescent protein (GFP⁺) and GFP^- mice revealed that these microglia...Full Text Available

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The influence of an immobilization stressor on lung cellular immune responses was studied. Delayed-type hypersensitivity to sheep erythrocytes was used to evaluate in vivo lung cellular immunity. Mice...Full Text Available

UK PubMed Central (United Kingdom)

In this study, our main objective was to determine whether energy restriction (ER) affects the rate of oxygen consumption of mice transiently or lastingly and whether metabolic rate plays a role in...Full Text Available

UK PubMed Central (United Kingdom)

An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly...Full Text Available

UK PubMed Central (United Kingdom)

The outcome of various osteoprogenitor-cell transplantation protocols was assessed using Col1a1-GFP reporter transgenic mice. The model requires the recipient mice to undergo lethal total body irradiation...Full Text Available

Science.gov (United States)

The results of a study of the expression of embryonic hemoglobin genes in mice which show an imbalance of alpha and non-alpha chain synthesis are reported. (ACR)

UK PubMed Central (United Kingdom)

Spontaneous mouse models of cancer show promise to more accurately recapitulate human disease and predict clinical efficacy. Transgenic mice or viral vectors have been required to generate spontaneous...Full Text Available

UK PubMed Central (United Kingdom)

Mice harboring a null mutation in Abca4/Abcr serve as a model of autosomal recessive Stargardt disease. Consistent with the human retinal disorder, deficiency...Full Text Available

UK PubMed Central (United Kingdom)

The aim of the present study was to evaluate the ameliorative potential of quercetin (QC) against paracetamol (PCM)-induced oxidative stress and biochemical alterations in mice blood. A total of 36...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

UK PubMed Central (United Kingdom)

We reported previously that Fas-induced hepatic failure in normal mice was attenuated or prevented by exogenous transferrin (Tf), particularly apoTf. Here we show in C57BL6J/129 mice with genetic...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

An animated test is made for mice with malignant tumors irradiated by a self-made millimetre wave radiator for medical purpose. It is observed that S-180 sarcomas in mice after irradiation has been distinctly suppressed. And remarkable effects are shown through a lot of clinical practices on peptic ulcer, skin-deep ulcer, acute and chromic soft tissue injuries etc.

Science.gov (United States)

The experiment was designed to study the effects of sodium pentobarbital on behavioral thermoregulation in rats and mice. Male Sprague-Dawley rats were given intraperitoneal (i.p.) injections of sodium pentobarbital in doses of 0, 1, 5, 10 or 15 mg/kg and male CBA/J mice were giv...

UK PubMed Central (United Kingdom)

BackgroundHerbivore feeding elicits dramatic increases in defenses, most of which require jasmonate (JA) signaling, and against which specialist herbivores are thought to be better...Full Text Available

UK PubMed Central (United Kingdom)

The purpose of this study was to determine the occurrence and genotypic relatedness of Salmonella enterica isolates recovered from feed and fecal samples in commercial swine production...Full Text Available

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Exposure of a group of horses to tetracycline-contaminated feed resulted in acute colitis and subsequent death in one horse and milder diarrhea in 3 others. The most severely affected animal demonstrated...Full Text Available

UK PubMed Central (United Kingdom)

Feed samples from 94 cases involving fungal contamination and suspected mycotoxicosis of farm animals in western Canada were examined during 1982-1994 to assess the incidence of mycotoxins. Samples...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundCichlid fishes in Lake Tanganyika exhibit remarkable diversity in their feeding habits. Among them, seven species in the genus Perissodus are known for...Full Text Available

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Bacillus thuringiensis was isolated from 36 of 50 residue samples obtained from an animal feed mill (a stored-product environment). Of 710 selected colonies having Bacillus...Full Text Available

UK PubMed Central (United Kingdom)

The association of breast feeding with rates of atopic illness during the first five years of life was assessed in a national study of 13 135 children studied during the first week and at age 5 years....Full Text Available

International Nuclear Information System (INIS)

The major feed-water line break accident is re-analyzed, which is based on Guangdong Daya Bay nuclear power station final safety analysis report, to justify the impacts of the decreasing of auxiliary feed-water flow rate on the safety margin in Daya Bay. The results showed that the accident analysis can meet the demands of acceptance criteria with the auxiliary feed-water flowrate decreasing from 45 m"3/h to 41.8 m"3/h, and enough safety margin is still retained

International Nuclear Information System (INIS)

... 2432 v. 43(1) SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS

KoreaScience (Korea)

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In this work feed hardware for fed-batch cultivation is presented (broth recycle feed injection system or BRFIS). BRFIS proved superior to conventional submerged or dripped feed systems in reducing dissolved oxygen (DO) oscillations during Escherichia coli fed-batch cultivation (5 min coefficient of variation of 0.7% for BRFIS as compared to 26% or greater for conventional feeding hardware in a 2 L test reactor). Hence, BRFIS is useful for fed-batch cultivation systems where the DO signal is used in measurement or control. PMID:12675613

Energy Technology Data Exchange (ETDEWEB)

Laboratory studies have been carried out to determine the toxicity of methiocarb pellets to wild trapped wood mice in order to provide some background data prior to any further evaluation of hazard in the field. In this study, wood mice were exposed to dry and to dampened methiocarb pellets in order to reproduce field trial application conditions. Field observations of methiocarb pellets indicate that the physical character changes under dry and wet weather conditions. This may affect their relative attractiveness and potential toxicity to wood mice. The laboratory assessment of exposed wood mice included measurement of brain esterase activities, methiocarb residues in selected mouse tissue, carcasses, and histological evaluation of kidney, liver and lungs.

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...Keeping chickens: a beginner's guide : Directgov - Environment and greener living chickens, feeding chickens, egg marking, registering a ...flock, battery hens Chickens; Department for Environment, Food and Rural Affairs; Registrations; Animals; Livestock; Local government; Local ...authorities A guide for people keeping chickens on the laws for registering and feeding them, and how to spot key diseases. A ...guide for people keeping chickens on the laws for registering and feeding them, and how to spot key diseases. Keeping chickens: ...

British Library Electronic Table of Contents (United Kingdom)

Eribulin is a new anticancer agent currently in Phase III clinical trials for the treatment of metastatic breast cancer. In the current studies, we have investigated the effects of P-glycoprotein (P-gp) on the in vivo disposition of eribulin using CF-1 abcb1a-deficient mice, and the influence of eribulin on P-gp-mediated efflux of digoxin in Caco-2 cells. Eribulin was administered intravenously and orally in both CF-1 wild-type and CF-1 abcb1a-deficient mice. P-gp-mediated efflux of digoxin in Caco-2 cell monolayers was measured in the presence of eribulin. The plasma exposure to eribulin was higher in CF-1 abcb1a-deficient mice than that in CF-1 wild-type mice after intravenous (IV) and oral (PO) administrations. The oral bioavailability of eribulin was 62.3% in CF-1 abcb1a-deficient mice...

Energy Technology Data Exchange (ETDEWEB)

The behavior of melter feed (a mixture of nuclear waste and glass-forming additives) during waste-glass processing has a significant impact on the rate of the vitrification process. We studied the effects of silica particle size and sucrose addition on the volumetric expansion (foaming) of a high-alumina feed and the rate of dissolution of silica particles in feed samples heated at 5 C/min up to 1200 C. The initial size of quartz particles in feed ranged from 5 to 195 {micro}m. The fraction of the sucrose added ranged from 0 to 0.20 g per g glass. Extensive foaming occurred only in feeds with 5-{micro}m quartz particles; particles {ge}150 {micro}m formed clusters. Particles of 5 {micro}m completely dissolved by 900 C whereas particles {ge}150 {micro}m did not fully dissolve even when the temperature reached 1200 C. Sucrose addition had virtually zero impact on both foaming and the ...

Science.gov (United States)

... estrogen receptor status. Cancer Lett., 253, 291-300. 39. Xin,X ... and resistant mice. Brain Res.Bull., 52, 235-242. 40. Foster,BA ...

Science.gov (United States)

An intact basement membrane (BM) is essential for the proper function, differentiation and morphology of many epithelial cells. The disruption or loss of this BM occurs during normal development as well as in the disease state. To examine the importance of BM during mammary gland development in vivo, we generated transgenic mice that inappropriately express autoactivating isoforms of the matrix metalloproteinase stromelysin-1. The mammary glands from these mice are both functionally and morphologically altered throughout development. We have now documented a dramatic incidence of breast tumors in several independent lines of these mice. These data suggest that overexpression of stromelysin-1 and disruption of the BM may be a key step in the multi-step process of breast cancer.

British Library Electronic Table of Contents (United Kingdom)

Nano-sized silica is a promising material for disease diagnosis, cosmetics and drugs. For the successful application of nano-sized material in bioscience, evaluation of nano-sized material toxicity is important. We previously found that nano-sized silica particles with a diameter of 70nm showed acute liver failure in mice. Here, we performed histological analysis of major organs such as the liver, spleen, lung, kidney, brain and heart in mice, chronically injected with 70-nm silica particles for 4weeks. Histological analysis revealed hepatic microgranulation and splenic megakaryocyte accumulation in these 70-nm silica particles treated mice, while the kidney, lung, brain and heart remained unaffected. Thus, liver and spleen appear to be the major target organs for toxicity by the chronic a...

UK PubMed Central (United Kingdom)

BackgroundCystinuria is the most common inherited cause of urinary tract stones in children. It can lead to obstructive uropathy, which is a major cause of renal...Full Text Available

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Serotonin is an important signaling molecule involved in the control of feeding in flies and other animals. In this study, a potential neurohemal release site for serotonin and the effects of exogenous serotonin on protein feeding were examined in the black blow fly, Phormia regina. A dense network of varicose neural processes exhibiting serotonin-like immunoreactivity was identified on the dorsal region of the thoracico-abdominal ganglion in P. regina. This dorsal region of the central nervous system is a likely site for the release of serotonin into the hemolymph. Circulating serotonin may have multiple systemic effects on fly physiology, including modulating or regulating feeding related processes and diuresis. Injections of exogenous serotonin reduced protein meal size in female flies ...

UK PubMed Central (United Kingdom)

AbstractThe efficacy of tilmicosin administered in the feed to control Actinobacillus pleuropneumoniae infections in pigs was evaluated through a multisite, multitrial...Full Text Available

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BackgroundThe aim of this report was to describe duodenal obstruction caused by a rubber foreign body in a cow.Case PresentationThe clinical, biochemical...Full Text Available

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... of CVACs, including tunneled catheters (Hickman or Broviac), peripherally inserted central catheters (also called PICC lines or long lines), ...

UK PubMed Central (United Kingdom)

C57BL/6ByJ (B6) and 129P3/J (129) mice have different alleles of Tas1r3, which is thought to influence gustatory transduction of sweeteners, but studies have provided conflicting...Full Text Available

Science.gov (United States)

The properties of a nonviable, aqueous ether-extracted Brucela abortus preparation, Bru-Pel, are described. In addition to inducing a "virus-type" interferon response and protecting mice against challenge with otherwise lethal doses of Semliki Forest virus, Bru-Pel is demonstrated to have potent antitumor properties in mice. These antitumor effects appear to be mediated by an increase in nonspecific resistance similar to that seen with other experimental antitumor agents. PMID:728911

UK PubMed Central (United Kingdom)

We have recently shown that viable Borrelia burgdorferi organisms induce a chronic infection associated with arthritis and carditis in severe combined immunodeficiency (scid) mice but not in immunocompetent...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Earlier studies have indicated that xylitol may increase the absorption and urinary excretion of dietary oxalate. It has also been indicated that xylitol increases the absorption of calcium. Intestinal absorption of lead, a divalent contaminant in the diet, is in many respects similar to that of calcium. The purpose of this study was to evaluate the effects of xylitol on the intestinal absorption of lead using two different approaches: the in situ ligated intestinal loop technique in cockerels and gastric gavage in mice.

UK PubMed Central (United Kingdom)

PurposePrevious studies have demonstrated that in 129α3Cx46^−/− mice, age-related nuclear cataract is formed. In...Full Text Available

International Nuclear Information System (INIS)

Chemical analyses were done on the abnormal hemoglobins of the five (101 x SEC)F_1 offspring of X- irradiated adult SEC mice to determine which hemoglobin genes were expressed in each hemoglobin variant. Three offspring of irradiated SEC males did not express either of the two kinds of #alpha#-chains normally found in all SEC mice. The deficient #alpha#-chain synthesis caused these mice to exhibit an #alpha#-thalassemia similar to human #alpha#-thalassemia. Scanning electron microscopy was used to show that many erythrocytes of mice with #alpha#-thalassemia have bizarre shapes; e.g. many erythrocytes appeared flattened or had thorny projections (acanthocytes). One mutant with a tandem duplication of a segment of chromosome 7 (site of locus determining #beta#-chain structure) produced twice as much SEC as 101 #beta#-chain polypeptides. One mutant that probably arose by non-disjunction of chromosome 7's ...

British Library Electronic Table of Contents (United Kingdom)

Background The objective of this study was to confirm provisional quantitative trait loci (QTL) for cocaine-induced locomotor activation, on chromosomes 1, 5, 6, 9, 12, 15, 16, 17, and 18, previously identified in the AXB/BXA recombinant inbred (RI) and AcB/BcA recombinant congenic (RC) strains of mice derived from A/J (A) and C57BL/6J (B6) progenitors. This was accomplished through a genetic analysis of cocaine-induced activity in an AxB6 F2 cross and a phenotypic survey across a panel of B6.A chromosome substitution strains (CSS) mice. Mice were tested for cocaine-induced activity, following administration of saline and cocaine (20?mg/kg), utilizing an open-field procedure. Results Among AxB6 F2 mice, differences in cocaine-induced activity were associated with loci on chromosome 1 (D1Mi...

UK PubMed Central (United Kingdom)

We followed 183 infants for two years, 31 of whom were breast fed less than three and a half months (median 70 days; short breast feeding group) and a further 31 of whom were exclusively breast fed...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

A C.sub.5 -900.degree. F. (C.sub.5 -482.degree. C.) liquid yield greater than 50 weight percent MAF feed coal is obtained in a coal liquefaction process wherein a selected combination of higher hydrogen partial pressure, longer slurry residence time and increased recycle ash content of the feed slurry are controlled within defined ranges.

Energy Technology Data Exchange (ETDEWEB)

The Project was started August 1, 1992. During these first two months, the Project Management plan was issued and approved during discussions at the Pittsburgh Energy and Technology Centre. Feed materials for the experimental work (with the exception of the second solvent) have been received. The analytical work up of these feed materials has been completed, and autoclave stability tests with the solvent and solvent fraction have been performed and analyzed. The Project is proceeding on schedule.

International Nuclear Information System (INIS)

Cinnabar, a naturally occurring mercuric sulfide (HgS), has long been used in combination with traditional Chinese medicine as a sedative for more than 2000 years. Up to date, its pharmacological and toxicological effects are still unclear, especially in clinical low-dose and long-term use. In this study, we attempted to elucidate the effects of cinnabar on the time course of changes in locomotor activities, pentobarbital-induced sleeping time, motor equilibrium performance and neurobiochemical activities in mice during 3- to 11-week administration at a clinical dose of 10 mg/kg/day. The results showed that cinnabar was significantly absorbed by gastrointestinal (G-I) tract and transported to brain tissues. The spontaneous locomotor activities of male mice but not female mice were preferentially suppressed. Moreover, frequencies of jump and stereotype-1 episodes were progressively decreased after 3-week oral administration ...

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The thin windows for the Muon Ionization Cooling Experiment (MICE) liquid Absorber will be fabricated from 6061-T6-aluminum. The absorber and vacuum vessel thin windows are 300-mm in diameter and are 180 mm thick at the center. The windows are designed for an internal burst pressure of 0.68 MPa (100 psig) when warm. The MICE experiment design calls for changeable windows on the absorber, so a bolted window design was adopted. Welded windows offer some potential advantages over bolted windows when they are on the absorber itself. This report describes the bolted window and its seal. This report also describes an alternate window that is welded directly to the absorber body. The welded window design presented permits the weld to be ground off and re-welded. This report presents a thermal FEA analysis of the window seal-weld, while the window is being welded. Finally, the results of a test of a welded-window are presented.

British Library Electronic Table of Contents (United Kingdom)

The involvement of dorsal root ganglia was studied in an in vivo model of experimental rabies virus infection using the challenge virus standard (CVS-11) strain. Dorsal root ganglia neurons infected with CVS in vitro show prolonged survival and few morphological changes, and are commonly used to study the infection. It has been established that after peripheral inoculation of mice with CVS the brain and spinal cord show relatively few neurodegenerative changes, but detailed studies of pathological changes in dorsal root ganglia have not previously been performed in this in vivo experimental model. In this study, adult ICR mice were inoculated in the right hindlimb footpad with CVS. Spinal cords and dorsal root ganglia were evaluated at serial time points for histopathological and ultrastru...

Energy Technology Data Exchange (ETDEWEB)

The Muon Ionization Cooling Experiment (MICE) focusing solenoid magnets focus the muon beam within the MICE cooling channel on a liquid or solid absorber that is within the warm bore of solenoid. The focusing magnet has a warm bore of 470 mm. his magnet consists of two coils 210-mm long that is separated by an aluminum mandrel that is 200 mm long. Each of the coils has its own leads. The coils may be operated in either the non-flip mode (solenoid mode with both coils at the same polarity) or the lip mode (quadrupole focusing mode where both coils are at opposite polarity). This report describes the focusing solenoid magnet design that will be built by the vendor. The progress on the construction of the first of the focusing magnets will also be discussed in this report. Ultimately three of these magnets will be built. These magnets will be cooled using a pair 1.5 W (at 4.2 K) pulse tube coolers.

British Library Electronic Table of Contents (United Kingdom)

Glycyrrhizin is a triterpene glycoside, a major active constituent of licorice (Glycyrrhiza glabra) root and numerous pharmacological effects like anti-inflammatory, anti-viral, anti-tumour and hepatoprotective activities has been attributed to it. In this study we evaluated the anti-inflammatory activities of glycyrrhizin in mice model of acute inflammation, carrageenan-induced pleurisy. We report here that glycyrrhizin (given at 10mg/kg i.p. 5min prior to carrageenan) exerts potent anti-inflammatory effects in this model. Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity which contained a large number of neutrophils (PMNs) as well as an infiltration of PMNs in lung tissues and subsequ...

British Library Electronic Table of Contents (United Kingdom)

Please cite this paper as: Ectopic mineralization of connective tissue in Abcc6-/- mice: effects of dietary modifications and a phosphate binder - a preliminary study. Experimental Dermatology 2008; 17: 203-207. Abstract: Pseudoxanthoma elasticum (PXE), a heritable multisystem disorder, is caused by mutations in the ABCC6 gene. We have developed a murine model for PXE by targeted inactivation of the corresponding mouse gene. A feature of this mouse model is ectopic mineralization of connective tissue capsule surrounding the bulb of vibrissae. This study was designed to investigate the effect of dietary sevelamer hydrochloride (Renagel), a phosphate binder, and specific mineral modifications on ectopic mineralization of connective tissue in Abcc6-/- mice. Three groups were fed a specific di...

British Library Electronic Table of Contents (United Kingdom)

Abstract Crouzon syndrome is characterized by premature fusion of sutures and synchondroses. Recently, the first mouse model of the syndrome was generated, having the mutation Cys342Tyr in Fgfr2c, equivalent to the most common human Crouzon/Pfeiffer syndrome mutation. In this study, a set of micro-computed tomography (CT) scannings of the skulls of wild-type mice and Crouzon mice were analysed with respect to the dysmorphology caused by Crouzon syndrome. A computational craniofacial atlas was built automatically from the set of wild-type mouse micro-CT volumes using (1) affine and (2) non-rigid image registration. Subsequently, the atlas was deformed to match each subject from the two groups of mice. The accuracy of these registrations was measured by a comparison of manually placed landma...

UK PubMed Central (United Kingdom)

Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection...Full Text Available

UK PubMed Central (United Kingdom)

Exercise promotes weight loss and improves insulin sensitivity. However, the molecular mechanisms mediating its beneficial effects are not fully understood. Obesity correlates with increased production...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe dynorphin-kappa opioid receptor (KOR) system regulates glial proliferation after sciatic nerve injury. Here, we investigated its role in cell proliferation following...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundIsoproterenol-induced cardiac hypertrophy in mice has been used in a number of studies to model human cardiac disease. In this study, we compared the transcriptional response...Full Text Available

UK PubMed Central (United Kingdom)

Studies in rodents have shown that male sexual function can be disrupted by fetal or neonatal administration of compounds that alter endocrine homeostasis, such as the synthetic nonsteroidal estrogen...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundAKXD recombinant inbred strains of mice have proven to be very useful in the identification of potential oncogenes and tumor suppressors involved in the development of...Full Text Available

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The number and type of stem cells in spleen and bone marrow of mice were determined after exposure to a single dose of 150 R on day 6, to a single dose of 500 R on day 6 or day 9 or to a fractionated dose of 150 R + 350 R on day 6 and 9. The stem cells were assayed on the basis of colony forming units (CFU) in spleen and of incorporation of iododeoxyuridine in spleen and bone marrow of lethally irradiated host mice. During the first month of life, the number of stem cells in non-irradiated mice increases markedly in bone marrow and slightly in spleen. Irradiation causes a long-lasting depression in stem cells, particularly in bone marrow and affecting preferentially erythropoietic precursor cells. Following a dose of only 150 R, the number of CFU in bone marrow is still below control levels 24 days later. An exposure to 500 R fractionated between day 6 and 9 has a markedly greater effect on stem cells in the spleen than the ...

UK PubMed Central (United Kingdom)

The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large...Full Text Available

UK PubMed Central (United Kingdom)

How an individual’s sex and genetic background modify cardiac adaptation to increased workload is a topic of great interest. We systematically evaluated morphological and physiological...Full Text Available

UK PubMed Central (United Kingdom)

Interleukin 12 (IL-12) activates natural killer (NK) and T cells with the secondary synthesis and release of interferon-gamma (IFN-gamma) and other cytokines. IL-12-induced organ alterations are reported...Full Text Available

UK PubMed Central (United Kingdom)

We have recently reported that progeroid Zmpste24^−/− mice, which exhibit multiple defects that phenocopy Hutchinson-Gilford progeria syndrome, show a profound dysregulation...Full Text Available

UK PubMed Central (United Kingdom)

It has been of interest whether and when the rearrangement of neuronal circuits can be induced after projection patterns are formed during development. Earlier studies using cats reported that the rearrangement...Full Text Available

UK PubMed Central (United Kingdom)

The activity of mice in their home cage is influenced greatly by the cycle of light and dark. In addition, home-cage activity shows remarkable time-dependent changes that result in a prominent temporal...Full Text Available

UK PubMed Central (United Kingdom)

Clinical and experimental evidence has demonstrated the potential role of probiotics in the prevention or treatment of inflammatory bowel disease. Probiotic clones with direct immunomodulatory activity...Full Text Available

UK PubMed Central (United Kingdom)

OBJECTIVENicotinamide adenine dinucleotides (NAD⁺ and NADH) play a crucial role in cellular energy metabolism, and a dysregulated NAD⁺-to-NADH ratio is implicated...Full Text Available

UK PubMed Central (United Kingdom)

Despite the health-related benefits of exercise, many people do not engage in enough activity to realize the rewards, and little is known regarding the genetic or environmental components that account...Full Text Available

UK PubMed Central (United Kingdom)

The ability to achieve tumor selective expression of therapeutic genes is an area that needs improvement for cancer gene therapy to be successful. One approach to address this is through the...Full Text Available

UK PubMed Central (United Kingdom)

Increased oxidative stress is associated with perinatal asphyxia and respiratory distress in the newborn period. Induction of nuclear factor erythroid 2 p45-related factor (Nrf2) has been shown to decrease...Full Text Available

UK PubMed Central (United Kingdom)

Male transgenic mice expressing the polyomavirus middle T (PyV-MT) gene exhibited growth and developmental abnormalities in prostatic and other urogenital epithelium. Expression of PyV-MT was directed...Full Text Available

UK PubMed Central (United Kingdom)

A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its...Full Text Available

UK PubMed Central (United Kingdom)

Muscular dystrophies (MDs) comprise a group of degenerative muscle disorders characterized by progressive muscle wasting and often premature death. The primary defect common to most MDs involves disruption...Full Text Available

UK PubMed Central (United Kingdom)

The use of infectious pathogens has allowed the detection of the development of synergism between pathogens and ubiquitous environmental chemical contaminants. This synergism has been demonstrated to...Full Text Available

UK PubMed Central (United Kingdom)

Retinal degenerative diseases, such as retinitis pigmentosa and Leber congenital amaurosis, are a leading cause of untreatable blindness with substantive impact on the quality of life of affected individuals...Full Text Available

UK PubMed Central (United Kingdom)

Recently, genome-wide association studies (GWAS) have linked the human LIN28B locus to height and timing of menarche [1-Full Text Available

UK PubMed Central (United Kingdom)

1. Interactions of pyrazole and ethanol were studied in three laboratory test procedures. They included sleeping time in mice, rotor rod balance in rats and lever pressing behaviour of rats. 2....Full Text Available

UK PubMed Central (United Kingdom)

Exposure of mice to 1000 ppm of vinyl chloride (VC), 6 hr/day, 5 days/week, caused some acute deaths with toxic hepatitis and marked tubular necrosis of the renal cortex. Starting the sixth month,...Full Text Available

UK PubMed Central (United Kingdom)

Hyperproliferation of the colonic epithelium, leading to expansion of colonic crypt progenitors, is a recognized risk factor for colorectal cancer. Overexpression of progastrin, a nonamidated and incompletely...Full Text Available

UK PubMed Central (United Kingdom)

Francisella tularensis is a highly infectious bacterium causing the zoonotic disease tularaemia. During its infectious cycle, F. tularensis is not only exposed to the...Full Text Available

UK PubMed Central (United Kingdom)

Background:The cardiac sodium–calcium exchanger (NCX1) is a key sarcolemmal protein for the maintenance of calcium homeostasis in the heart. Since heart failure...Full Text Available

UK PubMed Central (United Kingdom)

One of the major decisions that germ cells make during their development is whether to differentiate into oocytes or sperm. In mice, the germ cells’ decision to develop as male or female depends...Full Text Available

UK PubMed Central (United Kingdom)

Exercise is essential for health, yet the amount, duration, and intensity that individuals engage in are strikingly variable, even under prescription. Our focus was to identify the locations and effects...Full Text Available

UK PubMed Central (United Kingdom)

Keap1 regulates Nrf2 activity in response to xenobiotic and oxidative stresses. Nrf2 is an essential regulator of cytoprotective genes. Keap1-null mice are lethal by weaning age due...Full Text Available

UK PubMed Central (United Kingdom)

Studies in mammals have led to the suggestion that hyperglycemia and hyperinsulinemia are important factors both in aging and in the development of cancer. It is possible that the life-prolonging effects...Full Text Available

UK PubMed Central (United Kingdom)

The discovery that aerobic exercise increases adult hippocampal neurogenesis and can enhance cognitive performance holds promise as a model for regenerative medicine. This study adds two new...Full Text Available

UK PubMed Central (United Kingdom)

Deficits in prepulse inhibition (PPI) are a biological marker for schizophrenia. To unravel the mechanisms that control PPI, we performed quantitative trait loci (QTL) analysis on 1,010 F2 mice derived...Full Text Available

UK PubMed Central (United Kingdom)

We developed mice with germline endogenous expression of oncogenic Hras to study effects on development and mechanisms of tumor initiation. They had high perinatal mortality, abnormal...Full Text Available

UK PubMed Central (United Kingdom)

Both deficiency and excess of growth hormone (GH) are associated with increased mortality and morbidity. GH replacement in otherwise healthy subjects leads to complications, whereas individuals with...Full Text Available

UK PubMed Central (United Kingdom)

Mice lacking acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), an enzyme that catalyzes the terminal step in triacylglycerol synthesis, have enhanced insulin sensitivity and are protected from...Full Text Available

Science.gov (United States)

Antivenoms are the currently available agents for treatment of snake venom intoxication in humans. The development of therapeutic strategies employing more generally available drugs could improve treatment of invenomation by reducing hypersensitive reacti...

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The distribution of lead in lactating mice and suckling offspring was studied with whole body autoradiography at 4 and 24 h after a single intravenous injection of {sup 203}Pb (50 mmol Pb/kg) to the dams. In the lactating mice on day 14 of lactation, the highest uptake of radioactivity at 4 h after administration was recorded in renal cortex, skeleton and liver. A high uptake was also evident in the mannary gland. At 24 h after administration, the radioactivity had decreased in most organs except in the skeleton. In the suckling pups, exposed to lead only via dams` milk for 24 h, the highest level of radioactivity was present in the intestinal mucosa and a much lower level of radioactivity was present in the skeleton. The mammary glands from mice given three daily intravenous injections of 240 {mu}mol Pb/kg were examined with X-ray microanalysis. At 4 h after the last injection, lead was found associated with casein ...

UK PubMed Central (United Kingdom)

Whereas genetic factors are known to influence both susceptibility to carcinogens and the capacity to respond to specific antigenic stimuli, little is known regarding the influence of phenotype per...Full Text Available

UK PubMed Central (United Kingdom)

The objective of this study was to develop a specific immunological diagnostic assay for yellow disease in hyacinths, using monoclonal antibodies (MAbs). Mice were immunized with a crude cell wall preparation...Full Text Available

UK PubMed Central (United Kingdom)

The increased transmission and geographic spread of dengue fever (DF) and its more severe presentation, dengue hemorrhagic fever (DHF), make it the most important mosquito-borne viral disease of humans...Full Text Available

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OBJECTIVEPhosphoinositide 3-kinase enhancer A (PIKE-A) is a proto-oncogene that promotes tumor growth and transformation by enhancing Akt activity. However, the physiological functions...Full Text Available

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Huntington's disease (HD) is one of several neurodegenerative disorders caused by expansion of CAG repeats in a coding gene. Somatic CAG expansion rates in HD vary between organs, and the greatest instability...Full Text Available

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Mice were killed 7, 11, 19, and 27 weeks after infection with strains of Schistosoma mansoni from Puerto Rico, Brazil, St Lucia, and Tanzania. The percentage recovery of adult worms...Full Text Available

UK PubMed Central (United Kingdom)

Growing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white...Full Text Available

UK PubMed Central (United Kingdom)

Tick-borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. The disease is characterized by fever and debilitating encephalitis that can progress to chronic illness...Full Text Available

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CSV consists of a very complex of molecules and demonstrates significant cellular activities capable of stimulating immune functions in vivo. The purpose of this study was to analyze the effects of...Full Text Available

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AIM: To investigate atrial natriuretic peptide (ANP) secretion from gastric mucosa and the relationship between the ANP/natriuretic peptide receptor type A (NPR-A) pathway and diabetic gastroparesis.METHODS:...Full Text Available

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The amyloidoses are the prototype gain of toxic function protein misfolding diseases. As such, several naturally occurring animal models and their inducible variants provided some of the first...Full Text Available

UK PubMed Central (United Kingdom)

Even in healthy individuals, aging leads to deterioration in visual acuity, contrast sensitivity, visual field, and dark adaptation. Little is known about the neural mechanisms that drive the...Full Text Available

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Cerebral malaria is the most severe complication of human infection with Plasmodium falciparum. It was shown that Plasmodium berghei ANKA-induced cerebral malaria was...Full Text Available

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BackgroundObesity is one of the principal causative factors involved in the development of metabolic syndrome. AMP-activated protein kinase (AMPK) is an energy sensor that regulates...Full Text Available

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This study investigated the effect of copper as an antibacterial agent on the infectivity of Salmonella enterica serovar Typhimurium. Mice were infected orally with a standardized dose...Full Text Available

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The purpose of this study was to evaluate the toxicological effects of feeding the oils of Calophyllum inophyllum, Pentaclethra macrophylla and Terminalia catappa to rats. The effects on physical appearance, feed intake, weight gain, plasma and tissue cholesterol and triacyglycerol levels in rats with 5% of the oils in normal rat feed were determined. Weekly monitoring of the rats showed good physical appearance and steady weight gain, with no mortality recorded for the period of the study. Haematological analysis of the rats indicated that they were not anaemic. Histopathotogical examination of the sections of the heart, liver, kidney and spleen revealed moderate (T. catappa oil) to severe fatty change and necrosis in the liver. Glomerulonephrotic changes in the kidneys of rats fed with T...

International Nuclear Information System (INIS)

Object: To suppress a quantity of impurities in a coolant fed into a reactor vessel. Structure: The concentration of oxygen in a coolant flowing from a condensation desalting instrument into a feed and condensation piping is measured by an oxygen-concentration detector to feed its signal to an adjusting instrument. A degree of opening of an oxygen flow control valve to maintain the concentration of oxygen in the cooling water flowing within the pipe in the range from about 10 to about 200 ppb. Also, the concentration of oxygen in the cooling water fed to the desalting instrument is maintained at a level less than 2 ppb. Thereby, the total amount of iron flown into the vessel can be suppressed to a fine amount such as less than about 1 ppb. (Kawakami, Y.).

International Nuclear Information System (INIS)

Purpose: To stably control the reactor water level so as not to cause excess water feeding in a BWR type reactor. Constitution: A flow control valve is disposed to the exit of a feedwater pump for a nuclear reactor and the valve is controlled by a flow regulator to maintain the water level constant in the reactor. A signal from a water level controller is inputted to the flow regulator to thereby control the flow rate control valve. In this case, the flow regulator remains in a saturated state just after the starting of the feedwater pump, in which the pump flowrate is at 100% to result in an excess water feeding condition. In view of the above, a feedback circuit is provided to the flow regulator so that the saturated state is eliminated and the water feeding can be controlled directly from the water level controller. (Kamimura, M.).

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White seabream (Diplodus sargus) is today recognized as a potential species for Mediterranean aquaculture. Still, scarce information exists on weaning practices in order to reduce the live food period. This study aims to evaluate the effect of an early weaning on fish larvae digestive enzymes activities. In order to accomplish this, larvae were weaned with an inert diet at 20days after hatching (DAH) (feeding regime W20) and a control group was weaned at 27DAH (feeding regime W27). Before weaning, the onset and development of the main digestive enzymes were studied. The pattern of variation of digestive enzymes activities were analyzed at 0, 2, 9, 13 and 20DAH, and from then on at 0, 1 and 3weeks after diet introduction; that is, at 20, 27 and 41days for feeding regime W20, and days 27, 34...

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Three N-methylcarbamates of iodonitrobenzyl alcohols (4-iodo-2-nitrobenzyl alcohol 2, 5-iodo-2-nitrobenzyl alcohol 3 and 4-iodo-2,6-dinitrobenzyl alcohol 4) bearing [[sup 125]I] have been prepared and characterized for their lipophilicity, their reduction potentials and the in vivo lability of the radioiodine in healthy mice. Based upon these results, 2 and 4 were tested in tumor-bearing mice showing limited uptake of radioactivity in tumours and a tumor-to-blood ratio of less than 1. Consequently these particular carbamates are not satisfactory as hypoxia imaging agents. (author).

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Thermal neutron beam from the TNRF of JRR-3M was utilized for detection of mice lung cancer in vivo. Both the static and real-time neutron imaging were tried. Very strong thermal neutron beam is necessary to detect lung cancers because small changes are hidden by the very large attenuation of thermal neutron beam by the hydrogens in the tissues. The detection was successfully performed. Especially, the Super Eye of Hamamatsu Photonics contributed very effectively. Bone marrows and cartilages were also shown clearly. If an efficient and compact neutron source will become available in future, this method will grow up as a powerful tool for biomedical application. (author).

Science.gov (United States)

A factor produced by plerocercoids of the tapeworm Spirometra mansonoides is similar to human growth hormone (hGH) in that it stimulates body growth, binds to hGH receptors, cross-reacts with anti-hGH antibodies, and has lactogenic and insulin-like activities. The purpose of this study was to determine whether plerocercoid growth factor (PGF) is similar to hGH in expressing diabetogenic activity in the genetically obese (ob/ob) mouse. To determine an effective dose for use in the obese mice, the ability of daily injections of PGF to stimulate growth of phenotypically normal mice of the same strain was assessed in a 10-day weight gain assay. Injections of PGF stimulated a dose-dependent weight gain (r = 0.83) and 25 ng eq/day of PGF stimulated a response not significantly different from that produced by 100 micrograms of bovine growth hormone/day. Diabetogenicity was assessed using fasting blood glucose and glucose tolerance tests in obese ...

International Nuclear Information System (INIS)

By polymerase chain reaction with arbitrary primer (AP-PCR), the possibility of transmission of genome instability to somatic cells of the offspring (F_1 generation) from male parents of mice exposed to chronic low-dose #gamma#-radiation was studied. Male mice 15 days after exposure to 10-50 cGy were mated with unirradiated females. Biopsies were taken from tale tips of two month-old mice progeny for DNA separation. Primer in the AP-PCR was 20-mer oligonucleotide flanking the micro-satellite locus Atplb2 on chromosome 11 of the mouse. Comparative analysis of individual fingerprints of AP-PCR products on DNA-templates from the offspring of irradiated and unirradiated male mice revealed an increased variability of micro-satellite-associated sequences in the genome of the offspring of males exposed to 25 and 50 cGy. DNA-fingerprints of the offspring of male mice exposed to chronic ...

UK PubMed Central (United Kingdom)

Commercial feed rations containing sulfamethazine at the level of 110 ppm were fed for a period of 65 days to market pigs in a study simulating normal farm practices. The levels of sulfamethazine at...Full Text Available

UK PubMed Central (United Kingdom)

Although deep-sea cephalopods are key marine organims, their feeding ecology remains essentially unknown. Here, we report for the first time the trophic structure of an assemblage of these animals (19...Full Text Available

UK PubMed Central (United Kingdom)

OBJECTIVE--The survey aimed at studying the associations between prevalent respiratory symptoms in an occupational population and sickness absence due to respiratory disorders. METHODS--A cross sectional...Full Text Available

UK PubMed Central (United Kingdom)

Some pathological conditions with feeding pattern alterations, including obesity and Huntington disease (HD) are associated with hypothalamic dysfunction and neuronal cell death. Additionally, the hypothalamus...Full Text Available

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An extruder used by kneading stearic acid (St acid) into it for the production of polystyrene for injection molding was studied. Most disadvantageous of kneading methods, melt-feed method, was applied because this method enables the utilization of existing facilities. St acid is discolored when the temperature surpasses 270{sup 0}C in nitrogen atmosphere, and the contact with metals intensifies the discoloring. The discoloring is more intensified by the contact with nitriding steel than with stainless steel. Residence time is 85 seconds, and even the contact with nitriding steel does not discolor ST acid when the temperature is lower than 270{sup 0}C. In the melt-feed method, when the die is in a bad shape, it brings about dead space at the tip of the die and St acid holds up there. When the kneading is in bad condition (St acid and polymer are hard to mix because their viscosities are quite different), St acid gathers between the barrel wall ...

UK PubMed Central (United Kingdom)

Mass mortalities among seals and dolphins inhabiting contaminated marine regions have led to speculation about a possible involvement of immunosuppression associated with environmental pollution. To...Full Text Available

International Nuclear Information System (INIS)

Fermented oil palm empty fruit bunch, now known as 'Sterifeed' has been characterized by physico-chemical properties. It has also been proven to have an improved digestibility (by in vitro and in-vivo test) over the original material. The remaining important aspect of feed to be examined is the long term effect of feeding this material to animals. The size of fermentation media bags used was 0.5 - 1 kg/bag. In the large scale production of these materials, the numbers of bags were increased. The production at pilot scale level reinvestigated the basic processing parameters for the 1 kg/bag media and also performed a trial run for different sizes of bags. These include: 1) investigation on the growth of fungi on fermentation media subjected to different treatment times and the non treated media, 2) evaluation of the processing rate, 3) trial run processing of 25-50 MT oil palm EFB into feed, and 4) processing of different ...

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Little change was observed in primates fed 75 mg/kg adenine over the four-week period. Minimal tissue pathology was encountered upon completion of the study. In contrast to earlier studies in which oral feeding of adenine to dogs caused severe symptoms, a...

UK PubMed Central (United Kingdom)

In virtually all organisms, life expectancy is profoundly affected by caloric intake. For example, dietary restriction (DR; a feeding regimen of fewer calories compared to the ad libitum level without...Full Text Available

UK PubMed Central (United Kingdom)

A closed loop control process assures that a system performs within control limits. In closed loop control, the system's output feeds back directly to change the system's inputs. We describe an approach...Full Text Available

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BackgroundComplementary feeding is currently recommended after six months of age, when the nutrients in breast milk alone are no longer adequate to support growth. Few studies have...Full Text Available

UK PubMed Central (United Kingdom)

To assess the effects of increasing the mineral content of parenteral nutrition solutions on the biochemical and radiological indicators of metabolic bone disease of prematurity 27 neonates who required...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe MYC transcription factors are known to be involved in the biology of many human cancer types. But little is known about the Myc/microRNAs cooperation in the regulation...Full Text Available

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A membrane process utilizing dialysis and selective complexation by water-soluble polymers has been developed. This process, termed affinity dialysis, has been shown to be selectively extract and concentrate both cations and anions in a manner similar to ion exchange or solvent extraction. The selective removal of calcium from sodium with selectivity of about 30, removal of chromate ion from dilute streams, and separation of transition metal ions such as Cu/Fe and Cu/Zn have all been successfully demonstrated. Effects of different polymers, polymer concentration, temperature, and flow rates have been studied. The effect of increased polymer concentration is to increase product concentration if appropriate changes in feed, polymer solution, and strip flow rates are made. A continuous polymer solution recycle and regeneration system has been constructed and operated with Cu/Zn and chromate/chloride feed streams. Removal of over 95% of the desired ...

Science.gov (United States)

The insulin signaling pathway, involving protein kinase B (PKB) and mitogen-activated protein kinase (MAPK), mediates the biological response to insulin and several growth factors and cytokines. To investigate the correlation between glucose transporter (Glut) biosynthesis and the insulin signaling pathway activated by novel compounds of Liriope platyphylla (LP9M80-H), alterations in Glut and key protein expression in the insulin signaling pathway were analyzed in the liver and brain of ICR mice treated with LP9M80-H. An in vitro assay showed that the highest level of insulin concentration was observed in the LP9M80-H-treated group, followed by the LP-H, LP-M, LP-E, and LP9M80-C-treated groups. Therefore, LP9M80-H was selected for use in studying the detailed mechanism of the insulin signaling pathway in animal systems. In an in vivo experiment, LP9M80-H induced a significant increase in glucose levels and a decrease of insulin concentration in the blood of ...

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Tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1) have been shown to stimulate bone resorption in vitro. We have now investigated whether these cytokines also cause a similar action when administered in vivo. This was made possible by the adaptation of a newly developed technique that enables the continual assessment of bone resorption in vivo in mice by measuring urinary excretion of {sup 3}H from ({sup 3}H)tetracycline-prelabeled animals. Experiments using maneuvers known to influence bone resorption, such as a change in dietary calcium or administration of parathyroid hormone or dichloromethylenebisphosphonate, indicate that the technique is reliable and sensitive in mice. Daily intravenous administration of either recombinant human or recombinant murine TNF-alpha, as well as subcutaneous administration of recombinant human IL-1 alpha, were found to stimulate bone resorption in a dose-dependent manner. The effect was maximal ...

International Nuclear Information System (INIS)

CBA/Ca mice were infected by either the intravenous or intraperitoneal route with Mycobacterium microti and the subsequent changes in local macrophage populations examined. Following infection, the number of macrophages increased and they showed greater expression of both MHC Class II molecules. This response was not dependent on viability of the mycobacteria, in contrast to reports with other microorganisms such as Listeria. Studies in sublethally irradiated mice indicated that persistent antigen could give rise to a response after a period of host recovery which was radiation dose dependent. This procedure also highlighted differences in the regulation of different murine class II antigens in vivo, as seen by delayed re-expression of I-E antigens. Macrophage accessory cell function, as assessed by an in vitro T cell proliferation assay, correlated with Ia expression after fixation, but not after indomethacin treatment; this highlights the ...

British Library Electronic Table of Contents (United Kingdom)

Previous research utilizing the AcB/BcA recombinant congenic strains (RCS) of mice mapped provisional quantitative trait loci (QTLs) for the psychostimulant effects of nicotine to multiple regions on chromosomes 7, 11, 12, 14, 16, and 17. The current study was designed to confirm these QTLs in an A/J (A)??C57Bl/6J (B6) F2 cross and a panel of B6.A chromosome substitution strains (CSS). The panel of B6.A CSS consists of 21 strains, each carrying a different A/J chromosome on a B6 background. The A??B6 F2, CSS, A, and B6 mice were tested for sensitivity to the effects of nicotine on locomotor activity using a computerized open-field apparatus. In A??B6 F2 mice two QTLs were identified which confirm those previously observed in the AcB/BcA RCS. Significant differences in the expression of ...

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Introduction: We have evaluated the ability of a semi-automated, optomotor reflex method to assess drug-induced visual dysfunction, in albino and pigmented rats and mice. Methods: Male Han Wistar (HW) and Long Evans (LE) rats and mice (CD-1 and C57BL/6) were tested in a chamber formed by 4 computer monitors displaying a rotating vertical grating, to elicit head-tracking movements. The highest visible grating frequency was taken as the threshold of visual acuity, in cycles per degree (c/d). Animals received an intravenous infusion of either sodium iodate (50mg/kg) or 0.9% w/v NaCl (aq). They were tested 2h later, then re-tested daily for a further 3days. The time course of the effect was assessed in HW rats over a 6-week period, including electron microscopy, and immunohistochemical analysi...

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Exercise training associated with robust conditioning can be useful for the study of molecular mechanisms underlying exercise-induced cardiac hypertrophy. A swimming apparatus is described to control training regimens in terms of duration, load, and frequency of exercise. Mice were submitted to 60- vs 90-min session/day, once vs twice a day, with 2 or 4% of the weight of the mouse or no workload attached to the tail, for 4 vs 6 weeks of exercise training. Blood pressure was unchanged in all groups while resting heart rate decreased in the trained groups (8-18%). Skeletal muscle citrate synthase activity, measured spectrophotometrically, increased (45-58%) only as a result of duration and frequency-controlled exercise training, indicating that endurance conditioning was obtained. In groups which received duration and endurance conditioning, cardiac weight (14-25%) and myocyte dimension (13-20%) increased. The best conditioning protocol to promote physiological ...

International Nuclear Information System (INIS)

We studied the dose-response effects of #gamma#-irradiation on the weight gains of the body and the brain in ICR mice exposed to various doses of "6"0Co #gamma#-irradiation ranging from 0 to 1.5 Gy on day 13 of pregnancy (E13). We found that 0.5 Gy #gamma#-irradiation caused a significant reduction in brain weight but not in body weight among 6-week-old mice. Higher doses (1.0, 1.5 Gy) seriously inhibited body and brain development, resulting in significantly low weights at 6 weeks of age. A significantly lower brain weight among fetuses exposed to 1.5 Gy was found as early as 24 hours after exposure, while significant reductions in the body weight of these same fetuses appeared 3 days after exposure. The effects of radiation on brain and body development were similar for both males and females. (author).

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CoTreat, a new inorganic ion exchange media, has been studied in the laboratory to support its application as a pre- coat to existing Funda filters in THORP feed pond plant (Sellafield, UK). This is a novel way of application of CoTreat, which is usually utilized in fixed-bed ion exchange columns in a granular form. The results present the effect of operating conditions (CoTreat dose, pond water chemistry) on CoTreat performance for the removal of Co-57 tracer from simulated pond water. Major findings include the strong dependence of Co-57 decontamination factor (DF) on feed activity. At the 200 Bq/L feed level, the observed DF was 10-20 but rose to 1000 and above when the feed level was increased to 20000 Bq/L. Calcium present in the feed was found to decrease the DF at concentrations higher than 1 ppm. The laboratory studies showed significantly higher DF's than what has ...

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Recent investigations have identified elevated concentrations of polychlorinated dibenzo-p-dioxins (dioxins) in marine sediments and wildlife of Queensland, Australia. While it has been demonstrated that the contamination is widespread and predominantly land-based, limited information exists on the pathways and fate of these compounds within the near-shore marine system. This environment supports unique and threatened species including green sea turtles (Chelonia mydas). Adult green turtles are predominantly herbivorous, feeding on seagrass and algae. Apart from initial migration to feeding grounds (at {proportional_to}10 years of age) and intermittent migrations to breeding grounds (at {proportional_to}30-50 years and thereafter), green turtles remain and feed within relatively small home ranges. Long life-span (50 years or more), near-shore feeding grounds and highly specialized food requirements ...

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In Kanazawa, Japan, air pollution from a Mn treatment factory was found to affect local junior high school students, who showed reduced respiratory function relative to their exposure to Mn. The use of Mn as an antiknock ingredient in automobile fuels has recently been introduced, placing the general population at risk of chronic low-level exposure. There are few animal studies on the effects of compounds other than manganese chloride (MnCl{sub 2}). In the case of environmental pollution by Mn, the differing physiological effects of the various chemical forms of Mn must be considered. The present study therefore examined the differences in the effects of several Mn compounds on the physiology and behavior of mice by short-term oral administration.

British Library Electronic Table of Contents (United Kingdom)

The petroleum ether fraction (PEF) from the EtOH extract of flowers and buds of Chrysanthemum indicum was evaluated on antinociception in mice using chemical and thermal models of nociception. PEF administered orally at doses of 188 and 376mg/kg produced significant inhibitions on chemical nociception induced by intraperitoneal acetic acid, subplantar formalin or capsaicin injections and on thermal nociception in the tail-flick test and the hot plate test. In the pentobarbital sodium-induced sleep time test and the open-field test, PEF neither enhanced the pentobarbital sodium-induced sleep time nor impaired the motor performance, indicating that the observed antinociception was unrelated to sedation or motor abnormality. In a measurement of core body temperature, PEF did not affect temper...

Science.gov (United States)

We developed mice with germline endogenous expression of oncogenic Hras to study effects on development and mechanisms of tumor initiation. They had high perinatal mortality, abnormal cranial dimensions, defective dental ameloblasts, and nasal septal deviation, consistent with some of the features of human Costello syndrome. These mice developed papillomas and angiosarcomas, which were associated with Hras(G12V) allelic imbalance and augmented Hras signaling. Endogenous expression of Hras(G12V) was also associated with a higher mutation rate in vivo. Tumor initiation by Hras(G12V) likely requires augmentation of signal output, which in papillomas and angiosarcomas is achieved via increased Hras-gene copy number, which may be favored by a higher mutation frequency in cells expressing the oncoprotein. PMID:19416908

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The integral membrane protein synaptophysin is one of the most abundant polypeptide components of synaptic vesicles. It is not essential for neurotransmission despite its abundance but is believed to modulate the efficiency of the synaptic vesicle cycle. Detailed behavioral analyses were therefore performed on synaptophysin knockout mice to test whether synaptophysin affects higher brain functions. We find that these animals are more exploratory than their wild type counterparts examining novel objects more closely and intensely in an enriched open field arena. We also detect impairments in learning and memory, most notably reduced object novelty recognition and reduced spatial learning. These deficits are unlikely caused by impaired vision, since all electroretinographic parameters measur...

International Nuclear Information System (INIS)

The activity of tyrosine aminotransferase (TAT) in the liver of mice subjected simultaneously to ionizing radiation and to local inflammatory process showed in the first 24 hours of the experiment statistically significant changes resulting from summation of the harmful effects of both factors. In the second 24-hour period an evident tendency was observed for return of a normal circadian rhythm of the enzyme. This points to presence of an endogenous mechanism of suppression of the manifestations of significant desynchronization of the circadian rhythm. The paper contains also a trial of mathematical analysis of changes in TAT activity in the circadian rhythm, a description and analysis or the suppression process, qualitative and quantitative determination of the phenomenon of cumulation of the effects of powerful stress factors acting on the animal organism. The obtained results were compared with the results of investigations of TAT activity changes obtained in ...

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The simultaneous biosorption of Cr(VI) and Cu(II) on free Rhizopus arrhizus in a packed column operated in the continuous mode was investigated and compared to the single metal ion situation. The breakthrough curves were measured as a function of feed flow rate, feed pH, and different combinations of metal ion concentrations in the feed solutions. Column competitive biosorption data were evaluated in terms of the maximum (equilibrium) capacity in the column, the amount of metal loading on the R. arrhizus surface, the adsorption yield, and the total adsorption yield. In the single-ion situation the adsorption isotherms were developed for optimum conditions, and it was seen that the adsorption equilibrium data fit the noncompetitive Freundlich model. For the multicomponent adsorption equilibrium the competitive adsorption isotherms were also developed. The competitive Freundlich model for binary metal mixtures represented ...

British Library Electronic Table of Contents (United Kingdom)

An 8-week feeding trial was conducted to evaluate the use of local poultry by-product meal (PBM) in replacement of imported fishmeal in the diets of cobia, Rachycentron canadum. Six isolipidic (12%) and isoproteic (45%) experimental diets were formulated using PBM to replace fishmeal at 20, 40, 60, 80 and 100% dietary protein. Eleven juvenile cobia (initial mean weight of 30.7???0.78?g) were randomly stocked in 300-L circular fibreglass tanks and hand-fed based on the total biomass of each tank, twice a day at 0900?h and 1500?h. The fish were group weighed at 2-week intervals to monitor their growth performance in order to adjust the feeding ratio. At the end of the feeding trial, weight gains (WGs) ranging from 221 to 322% were obtained. The specific growth rate (SGR), WG and protein effi...

International Nuclear Information System (INIS)

The effect of nutrition on reproductive performance was studied in a series of experiments using two models. Increased supply of nutrients was based on feeding lupin grain as a supplement and measurements of testicular growth or ovulation rates were used to measure reproductive characteristics. Purified sources of protein and energy were provided to determine the macronutrient(s), supplied with lupin feeding, responsible for initiating increases in testicular growth and ovulation rate. Increases in both of these parameters were closely related to the supply of additional energy but not protein. The production of acetate and glucose resulting from supplementary feeding with 750 g lupins/d was measured using infusions of "1"4C tracers. Subsequently, infusions of glucose and acetate (individually and in combination) were found to produce similar increases in ovulation rate to those measured in ewes receiving lupins. It was ...

International Nuclear Information System (INIS)

A schematic diagram shows the various processing steps to be performed during feed preparation in the Hanford Waste Vitrification Plant (HWVP). The pretreated NCAW is transferred to the slurry receipt adjustment tank (SRAT) in the HWVP for concentration. Water removed during boildown is collected in the slurry mix evaporator condensate tank (SMECT). After waste treatment the water is returned to the storage tanks for eventual disposal. The pretreated Neutralized Current Acid Waste (NCAW) is concentrated in the SRAT to approximately 125 g waste oxides/ liter. Formic acid is then added at a controlled rate to improve rheological properties and achieve a required reduction/oxidation state. Reflux boiling is initiated and continued for several hours. The concentrated slurry is cooled and pumped to the slurry mix evaporator (SME). In the SME, glass frit is added, and the slurry is further concentrated to achieve a nominal composition of 500 g total oxides/liter. This ...

Science.gov (United States)

The ability to feed on vertebrate blood has evolved many times in various arthropod clades. Each time this trait evolves, novel solutions to the problem posed by vertebrate hemostasis are generated. Consequently, saliva of blood-feeding arthropods has proven to be a rich source of antihemostatic molecules. Vasodilators include nitrophorins (nitric oxide storage and transport heme proteins), a variety of peptides that mimic endogenous vasodilatory neuropeptides, and proteins that catabolize or sequester endogenous vasoconstrictors. A variety of platelet aggregation inhibitors antagonize platelet responses to wound-generated signals, including ADP, thrombin, and collagen. Anticoagulants disrupt elements of both the intrinsic and extrinsic pathways. Molecular approaches (termed 'sialomics') to characterize the full inventory of mRNAs transcribed in salivary glands have revealed a surprising level of complexity within a single species. Multiple ...

British Library Electronic Table of Contents (United Kingdom)

Francesco Colonnas Hypnerotomachia Poliphili (The Strife of Love in a Dream) of 1499 is a bizarre architectural-erotic fantasy whose imagery has often perplexed commentators. One particular banquet scene has defied scrutiny. This paper decodes the meal as a typical Renaissance banquet, as harmonious and balanced as any painting or edifice, yet offering an image of divine feeding. The flavors, ingredients and meal structure all reflect current culinary aesthetics as well as Neo-Platonic love theory, occult symbolism and humoral medicine. The banquet scene features foods and cooking methods which were popular in late 15th century Italy and yet includes exotic ingredients such as precious jewels and perfumes which were meant to denote divine feeding. For example, a confection served as a star...

International Nuclear Information System (INIS)

The prevailing severe shortage of animal feed in most of the developing countries could, to a considerable extent, be overcome through improved range management, which includes introduction of high yielding drought-resistant forage crops, development of adequate water conservation measures, and as far as possible growing annual forage crops on part of the vast areas of arable land currently left fallow each year. Year round measurements are essential for a good understanding of soil water and nutrients dynamics, which allow for adequate evaluation of pasture management alternatives. The methods most commonly used for moisture measurements in soil profiles are discussed because such measurements are likely to form an essential part of any investigation aimed at increasing animal feed production through the development of adequate pasture management practices. (author).

International Nuclear Information System (INIS)

Imatran Voima Oy , (IVO) operates two Russian designed nuclear power plants of type VVER440/213. Unit 1 has been operating since 1977 and unit 2 since 1981. First damage of feed water distribution (FWD) pipes was observed in 1989. In closer examinations FWD-pipe T-connection and distribution nozzles suffered from severe erosion corrosion damage. Similar damages have been found also in other VVER-440 type NPPs. In 1994 the first FWD-pipe was replaced by a new design mounted over the tube bundle instead of the old FWD-pipe, which was located inside the tube bundle. The purpose of this paper is to describe the new FWD-pipe and discuss its effects on the steam generator chemistry. (author)

British Library Electronic Table of Contents (United Kingdom)

Mesopelagic zooplankton may meet their nutritional and metabolic requirements in a number of ways including consumption of sinking particles, carnivory, and vertical migration. How these feeding modes change with depth or location, however, is poorly known. We analyzed fatty acid (FA) profiles to characterize zooplankton diet and large particle (>51?m) composition in the mesopelagic zone (base of euphotic zone ?1000m) at two contrasting time-series sites in the subarctic (station K2) and subtropical (station ALOHA) Pacific Ocean. Total FA concentration was 15.5 times higher in zooplankton tissue at K2, largely due to FA storage by seasonal vertical migrators such as Neocalanus and Eucalanus. FA biomarkers specific to herbivory implied a higher plant-derived food sou...

International Nuclear Information System (INIS)

TAPP-3 and 4 reactors use large number of Self Powered Neutron Detectors (SPNDs) for Neutronic lower measurement and control. To perform in-situ calibration of these detectors in select locations and to validate the reactor physics codes which predict flux at various points in the core, traveling in-core probes (TIP) are required. The TIP assembly consists of a miniature neutron sensitive detector. The detector is driven in and out of core using a mechanism which facilitates positioning of the detector anywhere inside a vertical tube (Central carrier tube of any of the six select Vertical Flux Units) in the core. TIP is driven through retractable feed mechanism for a stroke of 13 m. This paper describes the developmental efforts and the operational feedback of the retractable feed mechanism for the stroke of 13 m used at TAPP 3 and 4 reactor. (author)

International Nuclear Information System (INIS)

The lower condition factors for two catfish species in water heated by effluents from APCo's Glen Lyn, Virginia plant as compared to control areas probably is not due to lack of food as evidenced by macroinvertebrate sampling and the fact that only 4.3 percent of the catfish stomachs were empty. Results suggested that young-of-the-year channel catfish were nonselectively eating food organisms present at both control and heated stations. The difference between stomach contents of flatheat catfish from the heated site and fauna available at that site indicated that fish were either moving out of the heated area to feed or were selectively feeding on organisms (particularly mayflies) which were in the substrate or drifted into the heated area. Stomach contents of the two species and distribution and abundance of the macroinvertebrate benthos community are detailed.

Science.gov (United States)

A multivariate bioprocess control approach, capable of tracking a pre-set process trajectory correlated to the biomass or product concentration in the bioprocess is described. The trajectory was either a latent variable derived from multivariate statistical process monitoring (MSPC) based on partial least squares (PLS) modeling, or the absolute value of the process variable. In the control algorithm the substrate feed pump rate was calculated from on-line analyzer data. The only parameters needed were the substrate feed concentration and the substrate yield of the growth-limiting substrate. On-line near-infrared spectroscopy data were used to demonstrate the performance of the control algorithm on an Escherichia coli fed-batch cultivation for tryptophan production. The controller showed good ability to track a defined biomass trajectory during varying process dynamics. The robustness of the control was high, despite significant external ...

Energy Technology Data Exchange (ETDEWEB)

An assay system for the stem cell that colonizes the thymus and differentiates into T cells was developed, and by using this assay system the existence of two subpopulations of stem cells for T cell lineage was clarified. Part-body-shielded and 900-R-irradiated C57BL/6 (H-2b, Thy-1.2) recipient mice, which do not require the transfer of pluripotent stem cells for their survival, were transferred with cells from B10 X Thy-1.1 (H-2b, Thy-1.1) donor mice. The reconstitution of the recipient's thymus lymphocytes was accomplished by stem cells in the donor cells and those spared in the shielded portion of the recipient that competitively colonize the thymus. Thus, the stem cell activity of donor cells can be evaluated by determining the proportion of donor-type (Thy-1.1+) cells in the recipient's thymus. Bone marrow cells were the most potent source of stem cells. By contrast, when the stem cell activity was compared between spleen ...

UK PubMed Central (United Kingdom)

In both type 1 and type 2 diabetes, pancreatic islet dysfunction results in part from cytokine-mediated inflammation. The ubiquitous eukaryotic translation initiation factor 5A (eIF5A), which is the...Full Text Available

International Nuclear Information System (INIS)

The loss of lead in various kinds of biological materials after drying and ashing is studied by using "2"0"3Pb as tracer which is intravenously injected into mice. The experimental results show that although the oven ashing can be used for determining the lead in samples of lung, kidney, and liver, etc., the oxygen plasma and high pressure digestion are the most suitable methods for the determination of lead in biological materials. (author) 5 refs.; 2 tabs.

UK PubMed Central (United Kingdom)

Werner syndrome (WS) is a rare disorder characterized by the premature onset of several pathologies associated with aging. The gene responsible for WS codes for a RecQ-type DNA helicase and is believed...Full Text Available

International Nuclear Information System (INIS)

Lead-203 [52.1 h, 279 (80.1%) KeV] has been recognized as a potentially useful tracer for tumor specific radiopharmaceuticals due to its favorable nuclear and chemical properties. This paper reports the cyclotron production of lead-203 and the labelling of monoclonal antibody B72.3, conjugated with 2-(p-isothiocyanatobenzyl)DOTA, with lead-203 in 30% yield. In vivo biodistribution and stability studies in mice are being conducted.

UK PubMed Central (United Kingdom)

BackgroundAmplification of 3q26 is one of the most frequent genetic alterations in many human malignancies. Recently, we isolated a novel oncogene eIF-5A2 within...Full Text Available

UK PubMed Central (United Kingdom)

Anti-TAG-72 monoclonal antibodies target the tumor-associated glycoprotein (TAG)-72 in various solid tumors. This study evaluated the use of anti-TAG-72 monoclonal antibodies, both murine CC49...Full Text Available

UK PubMed Central (United Kingdom)

Chondroitin sulfate (CS) and dermatan sulfate (DS) containing N-acetylgalactosamine 4,6-bissulfate (GalNAc(4,6-SO₄)) show various physiological activities through interacting...Full Text Available

UK PubMed Central (United Kingdom)

Mice lacking Kv1.1 Shaker-like potassium channels encoded by the Kcna1 gene exhibit severe seizures and die prematurely. The channel is widely expressed in...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundApoptosis is a major mode of hematological tumor death after radiation. Early detection of apoptosis may be beneficial for cancer adaptive treatment. ^99mTc-HYNIC-annexinV...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The harmful effects of high-intensity electric fields on human beings are discussed. Formulas for determining the field strength of high voltage transmission lines and track lines and experimental data of these effects on mice are given. Protective measures from these hazards are introduced. 18 references, 7 figures, 5 tables.

UK PubMed Central (United Kingdom)

We have previously described a monoclonal antibody (FA6-152), obtained by immunizing mice with fetal human erythrocytes [Edelman, Vinci, Villeval, Vainchenker, Henri, Miglierina, Rouger, Reviron, Breton-Gorius,...Full Text Available