PERBANYAKAN VEGETATIF BIDARA UPAS (Merremia Mammosa (Lour.) Hallier F) KEBUN RAYA BOGOR [Vegetative Propagation of Bidara Upas ( Merremia Mammosa (Lour.) Hallier F)) at Bogor Botanical Garden

OpenAIRE

Cahyaningsih, Ria; Hidayat, Syamsul; Hidayat, Endang

2017-01-01

Bidara upas (Merremia mammosa (Lour.) Hall.f.) is a medicinal plant, , that traditionaly use by Indonesian and Malaysian people. Regarding Indonesia Biodiversity Strategy and Action Plan released by The National Development Planning Agency, it is included in the extinct medicinal plant list (2003). As the center for plant conservation, Bogor botanical garden conducted vegetative propagation study on this plant by air layering, cutting, and tuber cutting. The aim to this study is to find effec...

Bacteriology of Dried Meat (Kilishi) Hawked in Some Northern ...

African Journals Online (AJOL)

BSN

4 Department of Medical Laboratory Sciences, University of Nigeria, Nsukka, ... isolated from 5 samples showing a percentage of 2.2%. ... Keywords: Bacterial pathogens, dried meat, Bacteriology, Kilishi, Food-borne disease ... refrigeration and in reduced oxygen atmosphere ... pathogenic micro-organisms presenting the.

Potential Use of Endophytic Bacteria to Control Pratylenchus Brachyurus on Patchouli

OpenAIRE

Harni, Rita; Supramana, Supramana; Supriadi, Supriadi

2012-01-01

Pratylenchus brachyurus is an important parasitic nematode which significantly decreases quality and quantity of patchouli oil. One potential measure for controlling the nematode is by using endophytic bacteria. These bacteria also induce plant growth. This study aimed to evaluate the potential of endo-phytic bacteria to control P. brachyurus. The experiments were carried out in the Bacteriological Laboratory of the Plant Protection Department, Bogor Agricultural University, and the Laborator...

A Kinetic Experiment for the Biochemistry Laboratory.

Science.gov (United States)

Palmer, Richard E.

1986-01-01

Discusses the use of specific reactions of metabolic pathways to make measurements in the laboratory. Describes an adaptation of an experiment used in undergraduate biochemistry laboratories involving the induction of an enzyme in E. coli, as well as its partial purification and characterization. (TW)

Known structure, unknown function: An inquiry‐based undergraduate biochemistry laboratory course

Science.gov (United States)

Gray, Cynthia; Price, Carol W.; Lee, Christopher T.; Dewald, Alison H.; Cline, Matthew A.; McAnany, Charles E.

2015-01-01

Abstract Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry‐ and research‐based could simultaneously impart scientific knowledge and foster a student's research expertise and confidence. We have developed a year‐long undergraduate biochemistry laboratory curriculum wherein students determine, via experiment and computation, the function of a protein of known three‐dimensional structure. The first half of the course is inquiry‐based and modular in design; students learn general biochemical techniques while gaining preparation for research experiments in the second semester. Having learned standard biochemical methods in the first semester, students independently pursue their own (original) research projects in the second semester. This new curriculum has yielded an improvement in student performance and confidence as assessed by various metrics. To disseminate teaching resources to students and instructors alike, a freely accessible Biochemistry Laboratory Education resource is available at http://biochemlab.org. © 2015 The Authors Biochemistry and Molecular Biology Education published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology, 43(4):245–262, 2015. PMID:26148241

Known structure, unknown function: An inquiry-based undergraduate biochemistry laboratory course.

Science.gov (United States)

Gray, Cynthia; Price, Carol W; Lee, Christopher T; Dewald, Alison H; Cline, Matthew A; McAnany, Charles E; Columbus, Linda; Mura, Cameron

2015-01-01

Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry- and research-based could simultaneously impart scientific knowledge and foster a student's research expertise and confidence. We have developed a year-long undergraduate biochemistry laboratory curriculum wherein students determine, via experiment and computation, the function of a protein of known three-dimensional structure. The first half of the course is inquiry-based and modular in design; students learn general biochemical techniques while gaining preparation for research experiments in the second semester. Having learned standard biochemical methods in the first semester, students independently pursue their own (original) research projects in the second semester. This new curriculum has yielded an improvement in student performance and confidence as assessed by various metrics. To disseminate teaching resources to students and instructors alike, a freely accessible Biochemistry Laboratory Education resource is available at http://biochemlab.org. © 2015 The Authors Biochemistry and Molecular Biology Education published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology.

Known structure, unknown function: An inquiry?based undergraduate biochemistry laboratory course

OpenAIRE

Gray, Cynthia; Price, Carol W.; Lee, Christopher T.; Dewald, Alison H.; Cline, Matthew A.; McAnany, Charles E.; Columbus, Linda; Mura, Cameron

2015-01-01

Abstract Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry? and research?based could simultaneously impart scientific knowledge and foster a student's research expertise and confidence. We have developed a year?long undergraduate biochemistry laboratory curriculum wherein students determine...

A 13-week research-based biochemistry laboratory curriculum.

Science.gov (United States)

Lefurgy, Scott T; Mundorff, Emily C

2017-09-01

Here, we present a 13-week research-based biochemistry laboratory curriculum designed to provide the students with the experience of engaging in original research while introducing foundational biochemistry laboratory techniques. The laboratory experience has been developed around the directed evolution of an enzyme chosen by the instructor, with mutations designed by the students. Ideal enzymes for this curriculum are able to be structurally modeled, solubly expressed, and monitored for activity by UV/Vis spectroscopy, and an example curriculum for haloalkane dehalogenase is given. Unique to this curriculum is a successful implementation of saturation mutagenesis and high-throughput screening of enzyme function, along with bioinformatics analysis, homology modeling, structural analysis, protein expression and purification, polyacrylamide gel electrophoresis, UV/Vis spectroscopy, and enzyme kinetics. Each of these techniques is carried out using a novel student-designed mutant library or enzyme variant unique to the lab team and, importantly, not described previously in the literature. Use of a well-established set of protocols promotes student data quality. Publication may result from the original student-generated hypotheses and data, either from the class as a whole or individual students that continue their independent projects upon course completion. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(5):437-448, 2017. © 2017 The International Union of Biochemistry and Molecular Biology.

Identification of the students' critical thinking skills through biochemistry laboratory work report

Science.gov (United States)

Anwar, Yunita Arian Sani; Senam, Laksono, Endang W.

2017-08-01

This work aims to (1) identify the critical thinking skills of student based on their ability to set up laboratory work reports, and (2) analyze the implementation of biochemistry laboratory work. The method of quantitative content analysis was employed. Quantitative data were in the form of critical thinking skills through the assessment of students' laboratory work reports and questionnaire data. Hoyo rubric was used to measure critical thinking skills with 10 indicators, namely clarity, accuracy, precision, consistency, relevance, evidence, reason, depth, breadth, and fairness. The research sample consisted of 105 students (35 male, 70 female) of Mataram University who took a Biochemistry course and 2 lecturers of Biochemistry course. The results showed students' critical thinking skills through laboratory work reports were still weak. Analysis of the questionnaire showed that three indicators become the biggest problems during the laboratory work implementation, namely, lecturers' involved in laboratory work implementation, the integration of laboratory work implementation of learning in the classroom has not been done optimally and laboratory work implementation as an effort to train critical thinking skills is not optimal yet.

Recent developments in forestry and land use in Indonesia

NARCIS (Netherlands)

Meijer, W.

1974-01-01

Dr. W. Meijer, who is Dutch-born, worked in Indonesia from 1951 to 1958, first at Bogor, then at Pajakumbuh, Sumatra, and was Forest Botanist in Sabah for several years, revisited Indonesia with a National Science Foundation travel grant under an NSF-AID (Agency for International Development)

Customer Satisfaction Index Model on Three Level Of Socioeconomic Status In Bogor Case Study: Customer Satisfaction on Branded Cooking Oil Product

OpenAIRE

Budi Setiawan

2014-01-01

Customer satisfaction index models have been developed in many countries, including Indonesia. Those models were commonly not focused on the socioeconomic status (SES) of the customer, sothis condition could be a research gap. The aims of this research is to analyze the customer satisfaction index model of branded cooking oil product in Bogor, Indonesia based on SES established from the household monthly routine consumption. Questionnaires were used as primary data collection instrument in th...

Bacteriological detection of Salmonella in the presence of competitive micro-organisms. Bacteriological collaborative study IV amongst the National Reference Laboratories for Salmonella, the use of MSRV as selective enrichment

NARCIS (Netherlands)

Raes M; Nagelkerke N; Henken AM; MGB; IMA

2000-01-01

A fourth bacteriological collaborative study was organised by the Community Reference Laboratory for Salmonella. All National Reference Laboratories for Salmonella (NRLs) participated. This study had two objectives: 1) Evaluation of the results of the detection of different contamination levels of

ANALISIS EKONOMI DAN STRATEGI PENGELOLAAN EKOWISATA (STUDI KASUS KAWASAN WISATA GUNUNG SALAK ENDAH KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Pini Wijayanti

2008-12-01

Full Text Available Tourism has beeb a part of commodity that widely used by a region government as one finance development resources. Practically, the tourism development must be directed tosustainable tourism ,which is one form is ecological tourism (ecotorism. The data of tourism sector of Indonesia shows significant growth on domestic tour. The present government of Indonesia is striving for ecotourism activity which one of them is located in conservation area, hoping that it can give significant economic impact as well as perform conservation effort . bogor regency is a tourism object in Indonesia that placed not farm from Jakarta. From the potential natural resources view, bogor has many natural torism spot except puncak that one of them is salak endah mountain tourism area (GSE placed in west region .this area has ten tourism objects with steady increasing visit trend. The increasing tourism will certainly influence the tourismdevelopment itself and at last it will also impact the economic development in that region. The study concerning economic contribution of tourism activity on a certain region, and yet can not indicate the benfit of natural resources existence for recreation. A whole study about economic assessment includes economic impact assessment and environmental services assessment from a resource for an activity of recreation will give a comprehensive economic assessment , as arecommendation to formulate tourism management strategy in that region

26 CFR 1.611-1 - Allowance of deduction for depletion.

Science.gov (United States)

2010-04-01

... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Allowance of deduction for depletion. 1.611-1... depletion. (a) Depletion of mines, oil and gas wells, other natural deposits, and timber—(1) In general... mines, oil and gas wells, other natural deposits, and timber, a reasonable allowance for depletion. In...

Influence of religious organisations' statements on compliance with a smoke-free law in Bogor, Indonesia: a qualitative study.

Science.gov (United States)

Byron, M Justin; Cohen, Joanna E; Gittelsohn, Joel; Frattaroli, Shannon; Nuryunawati, Ramadhani; Jernigan, David H

2015-12-14

To explore the Bogor public's perspective on Muslim organisations' pronouncements against smoking and the effect of these pronouncements on compliance with a new smoke-free law in the context of a prosmoking social norm. Semistructured focus group discussions were conducted, transcribed, coded using ATLAS.ti software, and analysed using thematic content analysis. Photo elicitation was also used during the focus groups. Bogor, Indonesia. 11 focus groups (n=89), stratified by age, gender and smoking status, with members of the public (46 male, 43 female, ages 18-50). There was limited knowledge of and compliance with both the smoke-free law and the religious pronouncements. In most of the focus groups, smoking was described as a discouraged, but not forbidden, behaviour for Muslims. Participants described the decision of whether to follow the religious pronouncements in the context of individual choice. Some participants felt religious organisations lacked credibility to speak against smoking because many religious leaders themselves smoke. However, some non-smokers said their religion reinforced their non-smoking behaviour and some participants stated it would be useful for religious leaders to speak more about the smoke-free law. Religious organisations' pronouncements appear to have had a small effect, primarily in supporting the position of non-smokers not to smoke. Participants, including smokers, said their religious leaders should be involved in supporting the smoke-free law. These findings suggest there is potential for the tobacco control community to partner with sympathetic local Muslim leaders to promote common goals of reducing smoking and public smoke exposure. Muslim leaders' views on smoking would be perceived as more credible if they themselves followed the smoke-free law. Additionally, public health messaging that includes religious themes could be piloted and tested for effectiveness. These findings may also inform similar efforts in other Muslim

Glycobiology, How to Sugar-Coat an Undergraduate Advanced Biochemistry Laboratory

Science.gov (United States)

McReynolds, Katherine D.

2006-01-01

A second semester biochemistry laboratory has been implemented as an independent projects course at California State University, Sacramento since 1999. To incorporate aspects of carbohydrate biochemistry, or glycobiology, into our curriculum, projects in lectin isolation and purification were undertaken over the course of two semesters. Through…

The Rule of Organic Fertilizer on Fertilizer Efficiency and Requirement Rate for Vegetable Crop on Inceptisols Ciherang, Bogor

Directory of Open Access Journals (Sweden)

Ladiyani Retno Widowati

2009-09-01

Full Text Available Utilization of organic fertilizer is an alternative to increase soil health and supply a small amount of plant nutrient. Meanwhile agricultural soil in Indonesia commonly have low organic carbon content (<2% therefore application of organic fertilizer is recommended. Based on that, the aim of the research is to measure the effectiveness of organic fertilizer on caisim (Brassica chinensis L. growth, to measure the release of N-NO3- and N-NH4+, to determine the recommendation of organic fertilizer for caisim on Incentisols Ciherang, Bogor. The research had been conducted at Research and Soil Testing Laboratory and green house of Soil Research Institute, Laladon – Bogor on April to July 2008 using soil sample of Inceptisols Ciherang, Bogor. The treatments were: Control (no fertilizer, NPK, NPK + 500 kg organic fertilizer, ¾ NPK + 500 kg organic fertilizer, ½ NPK + 500 kg organic fertilizer, ¼ NPK + 500 kg organic fertilizer, organic fertilizer 500kg, organic fertilizer 1000 kg, NPK + 750 kg organic fertilizer, and NPK + 250 kg organic fertilizer. The five replications research had been conducted with Completely Randomized Design. The result indicated thats: (1 NPK fertilizer increased with addition of organic fertilizer approved by the evidence of increasing of plant high 2-10%, leaves number 1-2%, and crop production 16-36%. The relative agronomic effectiveness (RAE of treatment NPK+various rate of organic fertilizer were higher than NPK alone with value of 136-181%. (2 The release of NH4+-N and NO3--N from anorganic fertilizer (NPK treatment in four weeks incubation period showed balance proportion with N rate. Nitrogen released from organic fertilizer in the same incubation period are 5.39 mg NH4+-N kg-1 and 12.39 mg NO3- -N kg-1. (3 The best organic fertilizer rate based on fertilizer curve for Inceptisols Cicadas-Bogor having low C and N-organic is 560 kg organic fertilizer + NPK (300 kg Urea ha-1; 50 kg SP-36 ha-1; 50 kg KCl ha-1 produce

Myoglobin structure and function: A multiweek biochemistry laboratory project.

Science.gov (United States)

Silverstein, Todd P; Kirk, Sarah R; Meyer, Scott C; Holman, Karen L McFarlane

2015-01-01

We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure, students work with computer modeling and visualization of myoglobin and its homologues, after which they spectroscopically characterize its thermal denaturation. Students also study protein function (ligand binding equilibrium) and are instructed on topics in data analysis (calibration curves, nonlinear vs. linear regression). This upper division biochemistry laboratory project is a challenging and rewarding one that not only exposes students to a wide variety of important biochemical laboratory techniques but also ties those techniques together to work with a single readily available and easily characterized protein, myoglobin. © 2015 International Union of Biochemistry and Molecular Biology.

A biochemistry laboratory course designed to enhance students autonomy

Directory of Open Access Journals (Sweden)

T. Silva

2015-08-01

Full Text Available INTRODUCTION: Laboratory sessions are responsible for promoting instrumentation skills desirable in biochemistry and biochemistry related careers. They are traditionally based on experimental protocols that lead to the expected results, and students usually have not autonomy to plan and execute their experiments. GOALS: This work aimed to enhance a traditional biochemistry lab course, applying pre-lab quizzes on protein biochemistry and lab techniques in order to have students better prepared to plan, execute and interpret experiments. This approach also aims to bring the laboratory sessions into an inquiry-based environment capable to improve students’ independent capabilities in 2 autonomy domains: learning and communication. MATERIAL AND METHODS: Online quizzes are delivered one week before each laboratory session, containing questions regarding the experimental techniques and theoretical basis related to them. Laboratory activities are presented in an inquiry-based approach where the first class of each activity is dedicated to plan experiments in order to answer the research questions presented by instructors. Activities are also organized in order to enhance students’ autonomy. The first activity is the simplest and more instructor-controlled and the last one is the most complex and less driven, transferring gradually to students the responsibility for their decisions in laboratory, supporting students’ autonomy. RESULTS: Online quizzes allowed instructors to identify students’ difficulties and to timely intervene. Scientific reports presented by students at the end of each activity showed that they performed better on less driven activities in which autonomy support were more complex than in the instructor controlled activities. CONCLUSIONS: Scientific reports analysis reveals students capabilities related to different scopes of autonomy, such as: discuss different strategies; find multiple solutions to solve problems; make their

Biochemistry: from supermarket to laboratory

OpenAIRE

F. R. Freitas-Rego; M. G. Pereira; S. O. Loureiro; M. T. de Santana; R.G. Garrido; F. de S.R.G Garrido

2007-01-01

After new campi as Instituto Multidisciplinar em Saúde (IMS/UFBA) startedworking, it was necessary to develop practical classes using domestic reagents atBiochemistry to Pharmacy (IMS078). Firstly, students visited a supermarket to readnutritional information at label and select possible products to be used in class. Moreover,chemical processes and fermentation were discussed as different foods and drinks wereanalysed. Some food were token to laboratories so that biomole cules qualitative ana...

PERSEPSI MASYARAKAT LOKAL TERHADAP DAMPAK INDUSTRI PARIWISATA TAMAN SAFARI INDONESIA DITINJAU DARI KONSEP PEMBANGUNAN BERKELANJUTAN

Directory of Open Access Journals (Sweden)

Sitti Halumiah

2014-12-01

Full Text Available This study tries to identify and analyze the perception of local society towards the effect of tourism industry of Taman Safari Indonesia (TSI Cisarua-Bogor. The existence of such toursm activity has effected the local society and environment. This study aims at analyzing society’s perception towards the effect of such tourism industry of TSI Cisarua-Bogor. In analyzing such perception towards the effect of tourism industry at Indonesia Safari Park Bogor, descriptive qualitative, SWOT, and Quantitative Strategies Planning Matrix (QSPM used by seeing the direct and indirect impact as well as the continous strategy. The result of analysis shown that the existence of TSI Cisarua-Bogor tourism had given positive impact to the society particularly in economy aspect, but on the other hand it had also shown its negative impact particularly the condition of its environment. Furtheremore, there had been a formulation of strategy for empowering the local society economy and an optimalization of tourism potency for encouraging society’s ability in preserving the environment.Keywords: socio economic and environmental impacts, tourism, society’s perception, SWOT analysis,QSPM analysis

Culturing Security System of Chemical Laboratory in Indonesia

Directory of Open Access Journals (Sweden)

Eka Dian Pusfitasari

2017-04-01

Full Text Available Indonesia has experiences on the lack of chemical security such as: a number of bombing terrors and hazardous chemicals found in food. Bomb used in terror is a homemade bomb made from chemicals which are widely spread in the research laboratories such as a mixture of pottasium chlorate, sulphur, and alumunium. Therefore, security of chemicals should be implemented to avoid the misused of the chemicals. Although it has experienced many cases of the misuse of chemicals, and many regulations and seminars related to chemical security have been held, but the implementation of chemical security is still a new thing for Indonesian citizens. The evident is coming from the interviews conducted in this study. Questions asked in this interview/survey included: the implementation of chemical safety and chemical security in laboratory; chemical inventory system and its regulation; and training needed for chemical security implementation. Respondents were basically a researcher from Government Research Institutes, University laboratories, senior high school laboratories, and service laboratories were still ambiguous in distinguishing chemical safety and chemical security. Because of this condition, most Indonesia chemical laboratories did not totally apply chemical security system. Education is very important step to raise people awareness and address this problem. Law and regulations should be sustained by all laboratory personnel activities to avoid chemical diversion to be used for harming people and environment. The Indonesia Government could also develop practical guidelines and standards to be applied to all chemical laboratories in Indonesia. These acts can help Government’s efforts to promote chemical security best practices which usually conducted by doing seminars and workshop.

9 CFR 147.15 - Laboratory procedure recommended for the bacteriological examination of mycoplasma reactors. 11

Science.gov (United States)

2010-01-01

... Laboratory procedure recommended for the bacteriological examination of mycoplasma reactors. 11 11 Yoder, H. W., Jr., “Mycoplasmosis.” In: Isolation and Identification of Avian Pathogens. (Stephen B. Hitchner, Chairman, Charles H. Domermuth, H. Graham Purchase, James E. Williams.) 1980, pp. 40-42, Creative Printing...

Determinan Perilaku Penggunaan Kontrasepsi Pasca Persalinan di Wilayah Kerja Puskesmas Merdeka Kota Bogor

Directory of Open Access Journals (Sweden)

Agustina Agustina

2017-08-01

Full Text Available Contraceptive use by women of reproductive age nationwide showed decreased from 61.4% (2007 to 55.86% (in 2010. In West Java amounted 59.8%. Unmet need in Indonesia is 14% and in West Java is 11.1%. Achievement of the family planning program active participant in Bogor reached 78.61% and the achievement of a new family planning program postpartum still low at 49.53% (BPMPM, Bogor 2013. The Purpose of research is to find out the determinant behavioral of contraceptive use post-natal in health center Bogor 2014.This research is quantitative with the design "Cross Sectional" study sample group of mothers aged 15-49 are married, have babies ages 6 weeks to 12 months is 111 respondents. The sampling technique is purposive sampling. The results of the bivariate analysis showed there is relation antenatal/ Ante Natal Care contraceptive use with p=0.031 and the value of OR=0.2 (95% CI=0.44 to 0.9. This means that mothers during their pregnancy ≥4 times have a great opportunity for not using contraception post partum was 0.2 times. Suggested to the health nurse at the health center in order to improve quality of care through counseling activities on family planning and counseling about contraception postpartum starting from pregnancy and continues into the postpartum period.

Bacteriological Study of the Marine Water in the Coastal of the North Sulawesi Province, Indonesia

Directory of Open Access Journals (Sweden)

Lies Indah Sutiknowati

2006-11-01

Full Text Available The main objective of this research was to study the marine bacteriology of the coast of North Sulawesi. The study was accomplished by calculating the abundance of coliform, heterotrophic, and pathogenic bacteria, and analyzing the coexistence relationship between bacteria and phytoplanktons. This research, which included the sampling and laboratory works, has been carried out on 25 - 28 October, 2000. The results suggested that the abundance of each bacteria was as follows: coliform bacteria range between 227-5940 cfu/100 ml with averages 1814.1 cfu/100 ml, found in all stations; heterotrophic bacteria range between (1-82 x 103 cfu/ml with averages 12.1 x 103 cfu/ml, it was high density and has association with phytoplankton Trichodesmium thieubautii. It was also found 6 species of pathogen bacteria e.g. Aeromonas, Citrobacter, Proteus, Pseudomonas, Yersinia and Shigella. The presence of coliform and pathogen bacteria was indicator of low quality of the seawater in the sampling area. Based on bacteriological study, the North Sulawesi Coastal is not suitable for aquaculture and need treatment and controlled for further coastal exploitation.

Order of draw practices in venous blood sampling at clinical biochemistry departments in the Danish health care system

DEFF Research Database (Denmark)

Jacobsen, Katja Kemp; Brandt, Ida; Christensen, Anne Vindahl

2018-01-01

the procedures in venous blood sampling among clinical biochemistry departments to assess the uniformity of order of blood draw and adherence to international guidelines in the Danish health care system. METHODS: We collected venous order of draw procedures from 49 clinical biochemistry departments at 22 public...... 15189:2012 accreditation (p = .57). CONCLUSIONS: Venous order of draw procedures is diverse at Danish clinical biochemistry departments and show moderate adherence to international guidelines....

Education in Medical Biochemistry in Serbia.

Science.gov (United States)

Majkic-Sing, Nada

2010-06-01

Medical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia. Medical biochemistry laboratories and medical biochemists as a profession are part of Health Care System and are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists in Serbia dates from 1945, when Department of Medical Biochemistry was established at Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate branch was established, educating Medical Biochemists under a special program. Since 2006 the new five year undergraduate (according to Bologna Declaration) and postgraduate program of four-year specialization according to EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredits the programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Medical Biochemistry), successful completion of the profession exam at the Ministry of Health after completion of one additional year of obligatory practical training in medical laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by Serbian Chamber of Medical Biochemists.

Known Structure, Unknown Function: An Inquiry-Based Undergraduate Biochemistry Laboratory Course

Science.gov (United States)

Gray, Cynthia; Price, Carol W.; Lee, Christopher T.; Dewald, Alison H.; Cline, Matthew A.; McAnany, Charles E.; Columbus, Linda; Mura, Cameron

2015-01-01

Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry- and research-based could simultaneously impart scientific knowledge and foster a student's…

A Laboratory Course in Clinical Biochemistry Emphasizing Interest and Relevance

Science.gov (United States)

Schwartz, Peter L.

1975-01-01

Ten laboratory experiments are described which are used in a successful clinical biochemistry laboratory course (e.g. blood alcohol, glucose tolerance, plasma triglycerides, coronary risk index, gastric analysis, vitamin C and E). Most of the experiments are performed on the students themselves using simple equipment with emphasis on useful…

A semester-long project-oriented biochemistry laboratory based on Helicobacter pylori urease.

Science.gov (United States)

Farnham, Kate R; Dube, Danielle H

2015-01-01

Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically relevant enzyme--Helicobacter pylori (Hp) urease--the activity of which is absolutely required for the gastric pathogen Hp to colonize the human stomach. Over the course of the semester, students undertake a biochemical purification of Hp urease, assess the success of their purification, and investigate the activity of their purified enzyme. In the final weeks of the semester, students design and implement their own experiments to study Hp urease. This laboratory provides students with an understanding of the importance of biochemistry in human health while empowering them to engage in an active area of research. © 2015 The International Union of Biochemistry and Molecular Biology.

Raising Environmental Awareness through Applied Biochemistry Laboratory Experiments

Science.gov (United States)

Salman Ashraf, S.

2013-01-01

Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can provide solutions to such real-life issues. To this end, a newly developed biochemistry laboratory experiment…

An "in Silico" DNA Cloning Experiment for the Biochemistry Laboratory

Science.gov (United States)

Elkins, Kelly M.

2011-01-01

This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced…

Fluorescence quantum yield measurements of fluorescent proteins: a laboratory experiment for a biochemistry or molecular biophysics laboratory course.

Science.gov (United States)

Wall, Kathryn P; Dillon, Rebecca; Knowles, Michelle K

2015-01-01

Fluorescent proteins are commonly used in cell biology to assess where proteins are within a cell as a function of time and provide insight into intracellular protein function. However, the usefulness of a fluorescent protein depends directly on the quantum yield. The quantum yield relates the efficiency at which a fluorescent molecule converts absorbed photons into emitted photons and it is necessary to know for assessing what fluorescent protein is the most appropriate for a particular application. In this work, we have designed an upper-level, biochemistry laboratory experiment where students measure the fluorescence quantum yields of fluorescent proteins relative to a standard organic dye. Four fluorescent protein variants, enhanced cyan fluorescent protein (ECFP), enhanced green fluorescent protein (EGFP), mCitrine, and mCherry, were used, however the methods described are useful for the characterization of any fluorescent protein or could be expanded to fluorescent quantum yield measurements of organic dye molecules. The laboratory is designed as a guided inquiry project and takes two, 4 hr laboratory periods. During the first day students design the experiment by selecting the excitation wavelength, choosing the standard, and determining the concentration needed for the quantum yield experiment that takes place in the second laboratory period. Overall, this laboratory provides students with a guided inquiry learning experience and introduces concepts of fluorescence biophysics into a biochemistry laboratory curriculum. © 2014 The International Union of Biochemistry and Molecular Biology.

Kajian Daya Dukung Bioekologikawasan Puncak Kabupaten Bogor

Directory of Open Access Journals (Sweden)

Tika Rachmawati

2016-11-01

  ABSTRACT The area of research is the Puncak area in Bogor Regency, includes sub Ciawi, Cisarua and Megamendung as a part of the Regions Bogor, Puncak, Cianjur (Bopunjur and as a catchment and protected area (RTRW of Bogor Regency, period on 2005-2025. Purpose of this study were: (1 assess changes land use during the period 2005-2010 in the area of Puncak, Bogor regency, (2 examine the relationship of land use change on the carrying capacity of the environment and spatial planning bioekologi Bogor period on 2005-2025, and (3 formulate a strategy management of ecological land use at Puncak area.This research is descriptive qualitative and kuantatif, using secondary data analysis. The mathematical analysis of the ecological footprint approach (Global Footprint Network (GFN and the World Wildlife Fund (WWF  by calculating the value of ecological footprint, biocapacity and the carrying capacity of bioecology presented spatially.The results of this study are: (a the value of DDE in 2010 in the study area decreased from the value of DDE in 2005, and (b the difference in value of the existing DDE to the value of DDE RTRW is negative in almost all areas of research. This indicates that it was too broad land uses incompatible with existing land use plans in Bogor regency spatial planning and RTRW of Bogor Regency Year 2005-2025 is not accommodate the land use plan in the Puncak area. Formulation of strategies management the most appropriate use of land ecology are: (a reduce the area of land up around the summit region, especially in protected areas and conservation, forest rehabilitation also introduced a system of ecology-based tourism, (b optimizing spatial planning with spatial planning and control concept of spatial patterns, space utilization and integrated institutional, effective, and enforceable efiaien, and involve the community in monitoring and supervision so that the Puncak of development in the region, Bogor regency sustainable.

Implementation of quality management for clinical bacteriology in low-resource settings.

Science.gov (United States)

Barbé, B; Yansouni, C P; Affolabi, D; Jacobs, J

2017-07-01

The declining trend of malaria and the recent prioritization of containment of antimicrobial resistance have created a momentum to implement clinical bacteriology in low-resource settings. Successful implementation relies on guidance by a quality management system (QMS). Over the past decade international initiatives were launched towards implementation of QMS in HIV/AIDS, tuberculosis and malaria. To describe the progress towards accreditation of medical laboratories and to identify the challenges and best practices for implementation of QMS in clinical bacteriology in low-resource settings. Published literature, online reports and websites related to the implementation of laboratory QMS, accreditation of medical laboratories and initiatives for containment of antimicrobial resistance. Apart from the limitations of infrastructure, equipment, consumables and staff, QMS are challenged with the complexity of clinical bacteriology and the healthcare context in low-resource settings (small-scale laboratories, attitudes and perception of staff, absence of laboratory information systems). Likewise, most international initiatives addressing laboratory health strengthening have focused on public health and outbreak management rather than on hospital based patient care. Best practices to implement quality-assured clinical bacteriology in low-resource settings include alignment with national regulations and public health reference laboratories, participating in external quality assurance programmes, support from the hospital's management, starting with attainable projects, conducting error review and daily bench-side supervision, looking for locally adapted solutions, stimulating ownership and extending existing training programmes to clinical bacteriology. The implementation of QMS in clinical bacteriology in hospital settings will ultimately boost a culture of quality to all sectors of healthcare in low-resource settings. Copyright © 2017 The Authors. Published by

Integrating bio-inorganic and analytical chemistry into an undergraduate biochemistry laboratory.

Science.gov (United States)

Erasmus, Daniel J; Brewer, Sharon E; Cinel, Bruno

2015-01-01

Undergraduate laboratories expose students to a wide variety of topics and techniques in a limited amount of time. This can be a challenge and lead to less exposure to concepts and activities in bio-inorganic chemistry and analytical chemistry that are closely-related to biochemistry. To address this, we incorporated a new iron determination by atomic absorption spectroscopy exercise as part of a five-week long laboratory-based project on the purification of myoglobin from beef. Students were required to prepare samples for chemical analysis, operate an atomic absorption spectrophotometer, critically evaluate their iron data, and integrate these data into a study of myoglobin. © 2015 The International Union of Biochemistry and Molecular Biology.

Bacteriological detection of Salmonella in the presence of competitive micro-organisms (A collaborative study amongst the National Reference Laboratories for Salmonella)

NARCIS (Netherlands)

Voogt N; Veld PH in 't; Nagelkerke N; Henken AM; MGB

1997-01-01

A second bacteriological collaborative study in which the National Reference Laboratories (NRLs) for Salmonella participated was organized by the Community Reference Laboratory for Salmonella. The main objective of this study was to evaluate differences in results between the NRLs of detection of

[External quality assessment in clinical biochemistry laboratories: pilot study in 11 laboratories of Lomé (Togo)].

Science.gov (United States)

Kouassi, Kafui; Fétéké, Lochina; Assignon, Selom; Dorkenoo, Ameyo; Napo-Koura, Gado

2015-01-01

This study aims to evaluate the performance of a few biochemistry analysis and make recommendations to the place of the stakeholders. It is a cross-sectional study conducted between the October 1(st), 2012 and the July 31, 2013 bearing on the results of 5 common examinations of clinical biochemistry, provided by 11 laboratories volunteers opening in the public and private sectors. These laboratories have analysed during the 3 cycles, 2 levels (medium and high) of serum concentration of urea, glucose, creatinine and serum aminotransferases. The performance of laboratories have been determined from the acceptable limits corresponding to the limits of total errors, defined by the French Society of Clinical Biology (SFBC). A system of internal quality control is implemented by all laboratories and 45% of them participated in international programs of external quality assessment (EQA). The rate of acceptable results for the entire study was of 69%. There was a significant difference (plaboratories engaged in a quality approach and the group with default implementation of the quality approach. Also a significant difference was observed between the laboratories of the central level and those of the peripheral level of our health system (plaboratories remains relatively unsatisfactory. It is important that the Ministry of Health put in place a national program of EQA with mandatory participation.

Application of indices Cp and Cpk to improve quality control capability in clinical biochemistry laboratories.

Science.gov (United States)

Chen, Ming-Shu; Wu, Ming-Hsun; Lin, Chih-Ming

2014-04-30

The traditional criteria for acceptability of analytic quality may not be objective in clinical laboratories. To establish quality control procedures intended to enhance Westgard multi-rules for improving the quality of clinical biochemistry tests, we applied the Cp and Cpk quality-control indices to monitor tolerance fitting and systematic variation of clinical biochemistry test results. Daily quality-control data of a large Taiwanese hospital in 2009 were analyzed. The test items were selected based on an Olympus biochemistry machine and included serum albumin, aspartate aminotransferase, cholesterol, glucose and potassium levels. Cp and Cpk values were calculated for normal and abnormal levels, respectively. The tolerance range was estimated with data from 50 laboratories using the same instruments and reagents. The results showed a monthly trend of variation for the five items under investigation. The index values of glucose were lower than those of the other items, and their values were usually quality control, but also for revealing inter-laboratory qualitycontrol capability differences.

The Role of Student Engagement in the Success of Study of Scholarship Awardee Students of Bogor Agricultural University, Indonesia

Science.gov (United States)

Astuti, Farchaini Budi; Sumarwan, Ujang; Qayim, Ibnul

2016-01-01

This study aims to analyze the effect of student characteristics, internal factors and external factors on student engagement and the success of scholarship awardee's study in Bogor Agricultural University. The theory used in this study is that the success study of the scholarship awardee is affected by the student characteristics, internal and…

A Semester-Long Project-Oriented Biochemistry Laboratory Based on "Helicobacter pylori" Urease

Science.gov (United States)

Farnham, Kate R.; Dube, Danielle H.

2015-01-01

Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically…

Clinical biochemistry education in Spain.

Science.gov (United States)

Queraltó, J M

1994-12-31

Clinical biochemistry in Spain was first established in 1978 as an independent specialty. It is one of several clinical laboratory sciences specialties, together with haematology, microbiology, immunology and general laboratory (Clinical analysis, análisis clinicos). Graduates in Medicine, Pharmacy, Chemistry and Biological Sciences can enter post-graduate training in Clinical Chemistry after a nation-wide examination. Training in an accredited Clinical Chemistry department is 4 years. A national committee for medical and pharmacist specialties advises the government on the number of trainees, program and educational units accreditation criteria. Technical staff includes nurses and specifically trained technologists. Accreditation of laboratories is developed at different regional levels. The Spanish Society for Clinical Biochemistry and Molecular Pathology (SECQ), the national representative in the IFCC, has 1600 members, currently publishes a scientific journal (Química Clinica) and a newsletter. It organizes a continuous education program, a quality control program and an annual Congress.

[Blood volume for biochemistry determinations--laboratory needs and everyday practice].

Science.gov (United States)

Sztefko, Krystyna; Mamica, Katarzyna; Bugajska, Jolanta; Maziarz, Barbara; Tomasik, Przemysław

2014-01-01

Blood loss due to diagnostic phlebotomy jest a very serious problem, especially for newborn, infants and critically ill patients on intensive care units. Although single blood loss can be easily tolerated in adults, in small babies and in patients who are frequently monitored based on laboratory tests iatrogenic anaemia can occur. To evaluate the blood volume drawn for routine biochemistry tests in relation to patient age and the number of parameters requested. Blood volume drawn for routine biochemistry measurements from patients hospitalized in University Children's Hospital (N = 2980, children age from one day to 18 years) and in University Hospital (N = 859, adults, aged > 1.8 years) in Cracow has been analyzed. Blood volume was calculated based on regular tube diameter and blood heights in the tube. In case of microvettes the blood volume was 0.2 ml. Statistical analysis has been performed by using PRISM 5.0. The statistical significance was set at p nurses and doctors should include the information about current laboratory automation and methods miniaturization; 2) The amount of blood volume needed by laboratory for the requested number of tests should always be taken into account when diagnostic phlebotomy is necessary.

Strategi Bersaing Agroindustri Lapis Bogor Sangkuriang PT. Agrinesia Raya

OpenAIRE

Nuning Setyowati, Gayatri Zahra Sulthanah Mohamad Harisudin

2016-01-01

: This study aims to determine the critical success factors in marketing products Lapis Bogor Sangkuriang, to know the competitive position of products Lapis Bogor Sangkuriang compared with its competitors, and to find alternative competitive strategy that can be applied in marketing of Lapis Bogor Sangkuriang by PT. Agrinesia Raya. The basic method used was descriptive analysis method. Locations was intentionally selected (purposive) method. This study used primary and secondary data. Data a...

The Information Seeking Behavior of Digital Native and Digital Immigrant Students of Bogor Agricultural University

Directory of Open Access Journals (Sweden)

Janti Gristinawati Sujana

2018-02-01

Full Text Available Technological expansion and the changing way individuals gain access to information has deeply impacted the structure of libraries – physically as well as conceptually. A new generation of digital services platforms for libraries is emerging, designed to provide a more comprehensive  approach  to  the  management  and  access  to  all  formats  of  library  materials. Despite the modernization of libraries and their adaption to the digital age, the library still hold a critical role within community to serve its users, continuing to be beacons of information sharing, learning, and entertainment even amidst tight fiscal times.  As one of the leading university in Indonesia, Bogor Agricultural University Library must find solutions to new challenges, overhaul many of their entrenched business processes, and foster systems that engage students.  This study examined the information seeking behavior of the digital native and digital immigrant students of Bogor Agricultural University, in order to remind the library that there are some changes happened in its users and to recommend the new services should be taken by the library. The similarities and differences in seeking information of those two group students were discussed.

ANALISIS JANGKAUAN (OUTREACH LKMS BAGI RUMAHTANGGA MISKIN SEKTOR PERTANIAN DI PERDESAAN BOGOR, JAWA BARAT

Directory of Open Access Journals (Sweden)

Yani Mulyaningsih

2015-12-01

Full Text Available The Objective of research to acknowledge the hypothesis: outreach islamic microfinance for the poor household of agricultural sector at rural Bogor West Java. The majority of the poor community in Indonesia stay at rural areas. Lack of access credit/financing generally seen as one of the main reasons why many people remain poor. Credit/financing as a powerful instrument to help poor people break out of the vicious cycle because it has the potential to improve income and savings, consequently, enhance capital accumulation, and reinforce high incomes, but providing credit to the poor of agricultural sector in many cases is a very costly activity. Bank isn’t interest to serve it. Hopefully, islamic microfinance institutions reaches to more the poor of agricultural sector. The average loan is the most commonly used to measure the degree of microfinance institutions (MFIs outreach to poor customer segments, but it is very simple. Using the CGAP (Consultative Group to assist the poorest method, the poverty multidimension of islamic microfinance outreach was measured. The result of study showed that islamic MFIs didn’t reach the poor household of agricultural sector at rural Bogor West Java.

Gerakan Salafi Radikal dalam Konteks Islam Indonesia: Tinjauan Sejarah

Directory of Open Access Journals (Sweden)

Zuly Qodir

2014-01-01

Full Text Available This paper is aimed at describing the advent and development of the radical Salafi movement in Indonesia. Historically speaking, such movement was originated in the Middle East especially in Saudi Arabia, Jordan and Syria. In these countries movements like Hizbut Tahrir and Wahhabiya –to mention but few- were found and well-established. Movements such as these were actually banned subsequently in their countries of origin mainly for propagating the idea of Khilafah and for trying to topple the legitimate government. In Indonesia interestingly, the Salafi radical movements survived and even flourished amid the heavy criticism from their antagonists. Leading campuses in the country such as Bogor Institute for Agriculture (Institut Teknologi Bogor/IPB became their safe-heavens, as it were. Leaders and followers of Hizbut Tahrir Indonesia (HTI as well as Islamic Justice Welfare Party (PKS were graduates of this campus. Hence, it was on this campus that the seed of these Salafi movements was planted. The HTI and PKS must be considered the most important Salafi movements in modern Indonesia. They have played their important role in forming the history of this country. Views have been expressed concerning their nature and agendas. Some maintain that these movements are radical and fundamentalists and bear the political agenda to transform Indonesia into becoming the “Islamic State”. Others are of belief that these movements are moderate and progressive, or else revivalist and neo-fundamentalists. It is on this heated debate that this paper is interested in. It will explore the nature and agendas of these two Salafi movements by referring –first- to the views expressed by the experts, and –second- the views of the leadership of the Nahdhatul ‘Ulama (NU and Muhammadiyah, two largest Muslim organization in Indonesia.

Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor

Directory of Open Access Journals (Sweden)

Widagdo Sri Nugroho

2009-12-01

Full Text Available Johne’s disease (JD or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP. The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj, acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community.

A Survey on Faculty Perspectives on the Transition to a Biochemistry Course-Based Undergraduate Research Experience Laboratory

Science.gov (United States)

Craig, Paul A.

2017-01-01

It will always remain a goal of an undergraduate biochemistry laboratory course to engage students hands-on in a wide range of biochemistry laboratory experiences. In 2006, our research group initiated a project for "in silico" prediction of enzyme function based only on the 3D coordinates of the more than 3800 proteins "of unknown…

Biochemistry for dietetic students: course content and format.

Science.gov (United States)

Sirota, L H

1984-12-01

This article presents the results of a survey of the 251 undergraduate dietetic programs for course content and level of the biochemistry course most frequently used to satisfy competencies in biochemistry under Plan IV of the ADA in 1979-80. It showed that a common core of information was stressed by all biochemistry instructors, but there was great variability in content and level of material covered and the textbook chosen, depending on whether the biochemistry course was offered to dietetic majors only, in classes with other nonchemistry majors, or in classes with chemistry majors. Variability was also seen in the time allotted for biochemistry--39 to 280 hours (total lecture and required laboratory hours); laboratory requirements--only 71%; and departmental affiliation of the instructor--17 different departments, primarily of chemistry (80%), biology (8%), and home economics (4%). Topics given greatest emphasis were descriptive ones, such as definitions, simple structures, and reactions of intermediary metabolism in general terms. Topics given least emphasis were those involving mechanistic and quantitative biochemistry, such as respiratory quotient (RQ), enzyme kinetics, calculations of energy from fat and carbohydrates, and specific structures of vitamins, ketones, and metabolic intermediates. The lack of communication between biochemistry and nutrition instructors and the great differences in the preparation of dietetic majors in biochemistry are sources of concern.

An Experiment Using Sucrose Density Gradients in the Undergraduate Biochemistry Laboratory.

Science.gov (United States)

Turchi, Sandra L.; Weiss, Monica

1988-01-01

Describes an experiment to be performed in an undergraduate biochemistry laboratory that is based on a gradient centrifugation system employing a simple bench top centrifuge, a freezer, and frozen surcose gradient solution to separate macromolecules and subcellular components. (CW)

MULTI-SECTOR PARTNERSHIPS FOR SUSTAINABLE BUSINESS DEVELOPMENT: LESSONS LEARNED FROM ROUND TABLE INDONESIA

Directory of Open Access Journals (Sweden)

Huub Mudde

2012-09-01

Full Text Available Over a period of three years, Bogor Agricultural University/Institut Pertanian Bogor (IPB and Maastricht School of Management (MsM have been executing the multi-annual project Round Table Indonesia, www.roundtableindonesia.net. This project aimed at contributing to the improvement of a sustainable business and investment climate in the Indonesian agricultural sector, by strengthening the knowledge capacity, formulating concrete investment opportunities, and facilitating partnerships. As a result, IPB and MsM have developed courses on sustainable business development and facilitated business projects in poultry, mangosteen, palm oil, shrimps, and tourism. All projects are based on in-depth value chain analyses and roundtable meetings with key stakeholders of government, private sector, academia, and civil society. Linking education and applied research with business development will lead to a stronger and more sustainable Indonesian agricultural sector, being of crucial importance for the Indonesian development as a whole.Keywords: Round Table Indonesia, Sustainable Business Development, Value Chain, Agricultural Sector, IPB, MSM

Green Fluorescent Protein-Focused Bioinformatics Laboratory Experiment Suitable for Undergraduates in Biochemistry Courses

Science.gov (United States)

Rowe, Laura

2017-01-01

An introductory bioinformatics laboratory experiment focused on protein analysis has been developed that is suitable for undergraduate students in introductory biochemistry courses. The laboratory experiment is designed to be potentially used as a "stand-alone" activity in which students are introduced to basic bioinformatics tools and…

Pengaruh Kualitas Kerja Terhadap Kinerja Bank BNI Syariah Cabang Bogor

Directory of Open Access Journals (Sweden)

Barlian Wijaksono

2017-05-01

Full Text Available The purposes this research were analyzing the relationship between the characteristics of the employees and performance, analyzing the quality of the employee performance at Bank BNI Syariah Bogor, analyzing differences in the quality of the employee performance between male and female employees at Bank BNI Syariah Bogor, and as well as formulating the variables that can increase the quality of the performance of the employees of Bank BNI Syariah Bogor Branch. Based on the results in which the analysis used multiple linear regression, a conclusion can be taken from the study. The characteristics of the employees of BNI Syariah Bank which dominantly influences the performance include age range between 26–30 years, a university degree (S1, working year of 2–5 years, and frontliner position. Overall, the variables that affect the quality of the male and female employee performance of Bank BNI Syariah Bogor Branch include knowledge, working skill, behavior, and working experience. Based on the results, in terms of the quality the male employees, the variables that increase the performance quality at Bank BNI Syariah Bogor are only behavior and work experience, while for the female employees, there is no variable influencing the improvement of their performance quality in bank BNI Syariah Bogor. After conducting the testing on quality, the result shows that only male employees will increase their quality performance but only with variables of behavior and working experience.Keywords: gender, BNI Syariah, multiple linear regressions, quality of the employee, performanceABSTRAKTujuan dari penelitian ini yaitu menganalisis hubungan karakteristik karyawan dengan kinerja, menganalisis kualitas kinerja karyawan, menganalisis perbedaan kualitas kinerja karyawan antara gender laki-laki dengan perempuan, serta merumuskan variabel-variabel yang dapat meningkatkan kualitas kinerja karyawan Bank BNI Syariah Cabang Bogor. Penelitian ini menggunakan analisis

How do laboratory technicians perceive their role in tuberculosis diagnostic process: a cross-sectional study among laboratory technicians in health centers of Central Java Province, Indonesia

Directory of Open Access Journals (Sweden)

Widjanarko B

2016-09-01

Full Text Available Bagoes Widjanarko,1 Dyah Anantalia Widyastari,2 Martini Martini,3 Praba Ginandjar3 1Department of Health Education and Behavior Sciences, Faculty of Public Health, Diponegoro University, Semarang, Indonesia; 2Institute for Population and Social Research, Mahidol University, Salaya, Thailand; 3Department of Epidemiology, Faculty of Public Health, Diponegoro University, Semarang, Indonesia Purpose: Detection of acid-fast bacilli in respiratory specimens serves as an initial pulmonary tuberculosis (TB diagnosis. Laboratories are the essential and fundamental part of all health systems. This study aimed to describe how laboratory technicians perceived their own self and work. This included perceived self-efficacy, perceived role, perceived equipment availability, perceived procedures, perceived reward and job, and perceived benefit of health education, as well as level of knowledge and attitudes related to work performance of laboratory technicians.Methods: This was a cross-sectional quantitative study involving 120 laboratory technicians conducted in Central Java. Interviews and observation were conducted to measure performance and work-related variables.Results: Among 120 laboratory technicians, 43.3% showed fairly good performance. They complied with 50%–75% of all procedures, including sputum collection, laboratory tools utilization, sputum smearing, staining, smear examination, grading of results, and universal precaution practice. Perceived role, perceived self-efficacy, and knowledge of laboratory procedures were significantly correlated to performance, besides education and years of working as a laboratory technician. Perceived equipment availability was also significantly correlated to performance after the education variable was controlled.Conclusion: Most of the laboratory technicians believed that they have an important role in TB patients’ treatment and should display proper self-efficacy in performing laboratory activities. The

MINAT KEPEMILIKAN KARTU KREDIT (STUDI KASUS KOTA BOGOR

Directory of Open Access Journals (Sweden)

Bunga Ayu Lestari

2017-01-01

Full Text Available The growth of the credit card ownership in 2014 is not suitable with the projection of Bank Indonesia (BI.  This issue influences the program of the government regarding cash leash society and the effectiveness of BI’s regulation on credit card.  It is not clear whether the growth is from the new credit card owners or the existing owners.  This is possible because the marketers often offer the credit card to existing owners who have already credit cards.  The objective of the research is to analyze the factors of the people’s interest in possessing a credit card in Bogor.  The analysis used is Planned Behavior Theory, statistical analysis and structural equation modeling (SEM.  The result shows that only 17% of the people are interested in owning a credit card.  Based on the statistical analysis using SEM shows that behavior, norm, and controlling behavior are significant and positive to the owning of the credit card.  The behavior control through indicators presented  fewer people using credit card in the environment of consumer is a determinant  factor in influencing the interest on ownership of the credit card.Keywords: theory planned behavior (TPB, consumer behavior, SEM credit cardAbstrakPertumbuhan kepemilikan kartu kredit sepanjang tahun 2014 tidak sesuai dengan proyeksi Bank Indonesia (BI. Hal ini tentunya berdampak pada program pemerintah mengenai cash lessh society dan keefektifan peraturan BI terkait kartu kredit. Selain itu, belum diketahui juga secara pasti pertumbuhan tersebut datang dari pemilik baru atau justru pengguna eksisting. Hal ini dimungkinkan karena pihak marketing umumnya memasarkan kartu kredit pada nasabah yang sebelumnya telah memiliki kartu kredit. Tujuan dari penelitian ini adalah menganalisis faktor-faktor yang menentukan minat seseorang dalam memiliki kartu kredit dengan mengambil studi kasus kota Bogor. Analisis yang digunakan dalam penelitian ini adalah  Model Teori Planned Behavior (TPB

Exploring protein structure and dynamics through a project-oriented biochemistry laboratory module.

Science.gov (United States)

Lipchock, James M; Ginther, Patrick S; Douglas, Bonnie B; Bird, Kelly E; Patrick Loria, J

2017-09-01

Here, we present a 10-week project-oriented laboratory module designed to provide a course-based undergraduate research experience in biochemistry that emphasizes the importance of biomolecular structure and dynamics in enzyme function. This module explores the impact of mutagenesis on an important active site loop for a biomedically-relevant human enzyme, protein tyrosine phosphatase 1B (PTP1B). Over the course of the semester students guide their own mutant of PTP1B from conception to characterization in a cost-effective manner and gain exposure to fundamental techniques in biochemistry, including site-directed DNA mutagenesis, bacterial recombinant protein expression, affinity column purification, protein quantitation, SDS-PAGE, and enzyme kinetics. This project-based approach allows an instructor to simulate a research setting and prepare students for productive research beyond the classroom. Potential modifications to expand or contract this module are also provided. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(5):403-410, 2017. © 2017 The International Union of Biochemistry and Molecular Biology.

PROBABILITAS PULIH PADA BALITA KURUS DAN KURUS SEKALI MENURUT KEPATUHAN MENGIKUTI PEMULIHAN SECARA RAWAT JALAN DI KLINIK GIZI BOGOR

Directory of Open Access Journals (Sweden)

Sri Muljati

2012-11-01

Full Text Available THE PROBABILITY OF NUTRITIONAL STATUS IMPROVEMENT AMONG WASTING CHILDREN ACCORDING TO ROUTINE VISITS TO KLINIK GIZI BOGOR.Background: Prevalence of malnutrition on todler in Indonesia still high. It's an indication that nutrition prevention is not yet safisfiying. SKRT 2004 data shows that 9.3% todler in the cities and 11.1% In the village include in category at thin and very thin. Data from departement of health of RI 2005, Prevalence of malnutrition on todler is still on 19.2% and severe malnutrition is 8.8%. Bogor nutritional clinic is already develop severe malnutrition treament by out patient.Aims: To analize data of nutritional clinic visitors on 2001-2005. To now the probability of cured until Z_skore ≥ -2.5 SD dan Z_skore ≥ -2.0 SD, by using weight for height index on thin todlers and very thin todlers after six months of treatment. Methods: 294 todlers are devided in to two categories, which are 192 thin subject (≥-3SD s/d <-2 SD and 102 very thin (<-3 SD or thin with clinical indication. Inclution criteria in this anallsis, that the subject doesn't have such as down syndrome, heart disorder, thalasemia, hydrocephalus and microcephalus. Results: The study revealed that father education, mother age, todler age and compliance of treatment were correlated with probability of cured until Z_skore ≥ -2.5 SD dan Z_skore ≥ -2.0 SD, on thin todlers and very thin todlers after six months of treatment.Keywords: severe wasting, under five years, compliance

THE BOGOR BUS STATION RELOCATION IMPACT ANALYSIS TO THE BOGOR OUTER RING ROAD (BORR AND PANGERAN SOGIRI STREET PERFORMANCE

Directory of Open Access Journals (Sweden)

Tri Sudibyo

2015-02-01

Full Text Available As the main bus station in Bogor, West Java, Baranangsiang bus station is currently located in the crowded city centre, near many offices, campuses and department stores. There is a plan of Bogor’s Dinas Perhubungan Komunikasi dan Informasi (Diskominfo to relocate the bus station to the new location in the Pangeran Sogiri Street in Tanah Baru area, near the newly built toll road, the Bogor Outer Ring Road (BORR.This research is conducted to analyze the traffic performance impact of the relocation plan of the bus station to the BORR network and the Pangeran Sogiri Street. The traffic data is obtained from the field survey in the Baranangsiang bus station and the BORR network. Some other secondary data are obtained from the Dinas Lalu Lintas and Angkutan Jalan (DLLAJ Bogor city. Based on the obtained data, a micro simulation model is built by using Aimsun traffic micro simulation program. The analysis is conducted in three traffic performance parameters; the traffic density, speed, and vehicles queue length. With respect to the analysis result, the relocation causes a serious traffic problem to the BORR network and the P. Sogiri Street. To minimize the problem caused by the relocation, three scenarios are proposed and thus simulated based on the findings and observed traffic problems. The first proposed scenario is conducted by widening the P. Sogiri Street and providing a 4-4 toll payment gate system to the BORR. This first scenario is able to reduce traffic problem in some sections, but the average network density, speed and queue length are still high by 132%, -54%, and 200% respectively compared to the existing condition. The second scenario is conducted by widening the P. Sogiri Street and providing a 5-3 toll gate system. This second scenario is also able to reduce traffic problem in most sections, but the average network density, speed and queue length are still high by 248%, -70%, and 95% respectively compared to the main bus station

Biochemistry and radiopharmacy

International Nuclear Information System (INIS)

Mendoza de G, M.

1989-01-01

The article reviews the historical development of the nuclear medicine in Colombia and the primordial role of the IAN in this field. The main objective of the Biochemistry and Radiopharmacy Area is go to give technical support for the application of the nuclear energy in the human and veterinary medicine. The department has laboratories for the production of radiopharmaceuticals to be labelled with Tc-99m and quality control of the same human and veterinary RIA. Each one of the laboratories develops its work in three different areas: research and development, production, training and teaching. An actualization of the programs, results and publications are analyzed in this review also. Some of these programs have the support of the IAEA

Integrating Bio-Inorganic and Analytical Chemistry into an Undergraduate Biochemistry Laboratory

Science.gov (United States)

Erasmus, Daniel J.; Brewer, Sharon E.; Cinel, Bruno

2015-01-01

Undergraduate laboratories expose students to a wide variety of topics and techniques in a limited amount of time. This can be a challenge and lead to less exposure to concepts and activities in bio-inorganic chemistry and analytical chemistry that are closely-related to biochemistry. To address this, we incorporated a new iron determination by…

Ants in Tropical Urban Habitats: The Myrmecofauna in a Densely Populated Area of Bogor, West Java, Indonesia

Directory of Open Access Journals (Sweden)

AKHMAD RIZALI

2008-06-01

Full Text Available Ants are the most abundant animals in tropical habitats and have been widely studied in natural and semi-natural tropical systems. However, species in urban tropical habitats remain poorly studied, despite their abundance and potentially important roles in urban ecosystems and pest dynamics. We investigated the ant fauna of Bogor and its surroundings to contribute to the characterization of the myrmecofauna of one of Southeast Asia’s most densely populated regions. Ants were collected both by hand collection and from honey baits in the most common habitats: garbage dumps, households, and home gardens. In total, 94 species were recorded, over two thirds of which occurred in home gardens, which underlines the importance of vegetated habitats for urban planning to support complex ant assemblages. Twelve sampled species are well-known as tramp species that occur primarily in human-dominated landscapes. The two tramp species Anoplolepis gracilipes and Paratrechina longicornis dominated ant assemblages in all locations and most habitat types. The assemblages of tramp species were affected by habitat type, whereas that of non tramp species were not. Forty-five species were also recorded in the Bogor Botanical Garden and five species are also known to be common in cacao agroforests. Hence, research in urban tropical habitats can increase our knowledge of the occurrence of ant species, allowing us to better assess the biodiversity and conservation potential of semi-natural habitats.

Drug Synthesis and Analysis on a Dime: A Capstone Medicinal Chemistry Experience for the Undergraduate Biochemistry Laboratory

Science.gov (United States)

Streu, Craig N.; Reif, Randall D.; Neiles, Kelly Y.; Schech, Amanda J.; Mertz, Pamela S.

2016-01-01

Integrative, research-based experiences have shown tremendous potential as effective pedagogical approaches. Pharmaceutical development is an exciting field that draws heavily on organic chemistry and biochemistry techniques. A capstone drug synthesis/analysis laboratory is described where biochemistry students synthesize azo-stilbenoid compounds…

Bacteriological Evaluation of Kwale General Hospital Environment ...

African Journals Online (AJOL)

... Pharmacy (40.7%) and Theatre (18.5%). This study showed that Kwale General Hospital environment is heavily contaminated and therefore underlies the necessity for regular evaluation of the hospital environment. Keywords: Bacteriological evaluation, hospital, environment. Journal of Medical Laboratory Sciences Vol.

Thermodynamic Exploration of Eosin-Lysozyme Binding: A Physical Chemistry and Biochemistry Laboratory Experiment

Science.gov (United States)

Huisman, Andrew J.; Hartsell, Lydia R.; Krueger, Brent P.; Pikaart, Michael J.

2010-01-01

We developed a modular pair of experiments for use in the undergraduate physical chemistry and biochemistry laboratories. Both experiments examine the thermodynamics of the binding of a small molecule, eosin Y, to the protein lysozyme. The assay for binding is the quenching of lysozyme fluorescence by eosin through resonant energy transfer. In…

DISFEMIA DALAM BERITA UTAMA SURAT KABAR POS KOTA DAN RADAR BOGOR

OpenAIRE

Pratiwi, Kania; Ridwan, Sakura; Rahmawati, Aulia

2016-01-01

Abstrak. Penelitian ini bertujuan memahami secara mendalam penggunaan bentuk dan nilai rasa disfemia pada berita utama surat kabar Pos Kota dan Radar Bogor. Metode yang digunakan dalam penelitian ini adalah metode deskriptif kualitatif dengan teknik analisis isi. Hasil penelitian yang diperoleh dari surat kabar Pos Kota dan Radar Bogor ditemukan 245 data. Sebanyak 155 data atau 63,26% ditemukan pada surat kabar Pos Kota dan 90 data atau 36,73% ditemukan pada surat kabar Radar Bogor. Disfemia ...

Evaluation of roadside greenbelt trees damage caused by strangler plants in Bogor

Science.gov (United States)

Danniswari, Dibyanti; Nasrullah, Nizar

2017-10-01

Certain plants are called stranglers (hemiepiphyte) because they grow on host trees and slowly choking the host, which often results in the host’s death. The existence of strangler plants on roadside greenbelt trees is quite common in Bogor, but they may cause tree’s failure and threaten users’ safety. To prevent such hazard, evaluation of roadside greenbelt trees damage caused by strangler plants is important. This study was directed to analyse the vegetation of strangler plants in Bogor, to assess the damage caused by stranglers, and to compose strangled trees maintenance recommendations. This study was conducted in March to May 2014 by doing survey at five major roads in Bogor, which were Jalan Ahmad Yani, Jalan Sudirman, Jalan Pemuda, Jalan Semeru, and Jalan Juanda. The results showed that strangler species found in Bogor are Ficus benjamina, Ficus glauca, Ficus elastica, and Schefflera actinophylla. The most common species in Bogor is F. benjamina. Host trees that tend to be preferred by strangler plants are trees with large trunk, many branches, and medium to high height. The maintenance for every strangled tree is different according to the damage level, mild to severe damage could be treated by strangler root cutting to tree logging, respectively.

KEBIASAAN DAN KUALITAS SARAPAN PADA SISWI REMAJA DI KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Anna Febritta Intan Sari

2013-10-01

Full Text Available ABSTRACTThe study aimed to describe breakfast habit among teenage girls of vocational high school Bogor. A cross-sectional study was conducted and was involving 68 students 14—18 years old from SMK Pelita Ciampea, Bogor District. Breakfast consumption was collected by a 7-day food records. The result showed that there were 45.6% schoolgirls having breakfast regularly. Breakfast frequency per week was associated with BMI and anaemic status (pKey words: breakfast, concept, quality, schoolgirlsABSTRAKPenelitian ini bertujuan untuk mempelajari kebiasaan sarapan pada remaja siswi sekolah menengah kejuruan Bogor. Desain cross-sectional digunakan dengan subjek 68 siswi berusia 14—18 tahun dari SMK Pelita Ciampea, Kabupaten Bogor. Konsumsi sarapan dikumpulkan dengan food record selama 7 hari. Hasil penelitian menunjukkan bahwa 45.6% remaja siswi biasa sarapan setiap hari. Frekuensi sarapan dalam satu minggu berhubungan dengan IMT dan status anemia (pKata kunci: konsep, kualitas, sarapan, siswi remaja

Exploring Protein Structure and Dynamics through a Project-Oriented Biochemistry Laboratory Module

Science.gov (United States)

Lipchock, James M.; Ginther, Patrick S.; Douglas, Bonnie B.; Bird, Kelly E.; Loria, J. Patrick

2017-01-01

Here, we present a 10-week project-oriented laboratory module designed to provide a course-based undergraduate research experience in biochemistry that emphasizes the importance of biomolecular structure and dynamics in enzyme function. This module explores the impact of mutagenesis on an important active site loop for a biomedically-relevant…

STRATEGI PENINGKATAN PRODUKSI AGROINDUSTRI PEMBENIHAN LELE DI BOGOR

Directory of Open Access Journals (Sweden)

Kunandi Kunandi

2014-09-01

Full Text Available ABSTRACTThe study aimed to 1 identify internal and external factors that influence the strategy to increase the production of  catfish hatchery agro-industry,  2 identify and determine the factors, actors, goals and the alternative strategy to increase the production capacity of catfish hatchery agro-industry, 3 determine strategy priority in increasing the production capacity and operational sustainability of catfish hatchery agro-industry.  Methods of analytical hierarchy process and focus group discussions were used in the data analysis. The result showed that capital and main actors are the main factors that determine the production increase of catfish hatchery agro industry in Bogor. The main purpose of the increase in hatchery production of catfish in Bogor agro-industry is to create more job opportunities and the main strategy for catfish hatchery production is the human resources training.Keywords: AHP, agroindustry catfish, catfish hatchery, focus  group discussions/FGDABSTRAKTujuan dari  penelitian ini adalah 1 mengidentifikasi  faktor-faktor internal dan  eksternal yang memengaruhi strategi peningkatan kapasitas produksi agroindustri pembenihan lele di Bogor, 2 mengidentifikasi dan menetapkan elemen-elemen, seperti  faktor/kriteria, aktor, tujuan, dan alternatif strategi yang memengaruhi dalam peningkatan kapasitas produksi usaha pembenihan lele, dan 3 merumuskan prioritas strategi bisnis dalam peningkatan kapasitas produksi agroindustri pembenihan lele dan kelangsungan operasionalnya. Metode analisis data menggunakan analytical hierarchy process dan focus group discussions. Hasil penelitian menunjukkan bahwa faktor yang menentukan peningkatan produksi agroindustri pembenihan lele di Bogor adalah modal dan pelaku utama dalam pembenihan peternak lele. Tujuan utama dari peningkatan pembenihan agroindustri produksi lele di Bogor adalah menciptakan lapangan kerja. Sementara itu, strategi utama untuk memproduksi pembenihan ikan

Customer Satisfaction Index Model on Three Level Of Socioeconomic Status In Bogor Case Study: Customer Satisfaction on Branded Cooking Oil Product

Directory of Open Access Journals (Sweden)

Budi Setiawan

2014-06-01

Full Text Available Customer satisfaction index models have been developed in many countries, including Indonesia. Those models were commonly not focused on the socioeconomic status (SES of the customer, sothis condition could be a research gap. The aims of this research is to analyze the customer satisfaction index model of branded cooking oil product in Bogor, Indonesia based on SES established from the household monthly routine consumption. Questionnaires were used as primary data collection instrument in this study, while data analysis was carried out with variance based structural equation modeling (SEM which is also known as Partial Least Square (PLS model, and Kruskall Wallis nonparametric test. Perceived quality, perceived value and customer expectation as were significantly influencing the customer satisfaction construct in the structural model. This study also concluded that there is different level of overall customer satisfaction on the three levels of customer’s SES

PENGARUH GUIDE PERFORMANCE DAN QUALITY TOURISM SERVICE TERHADAP REVISIT INTENTION DI KEBUN RAYA BOGOR

Directory of Open Access Journals (Sweden)

Rian Andriani

2016-11-01

ABSTRACT Bogor Botanical Gardens is one of tourist destination in Bogor City, West Java, which the geographical in a strategic location between two big cities, Jakarta and Bandung. This location factor is can become an opportunity for Bogor Botanical Gardens to become a favorite tourist destination in Bogor City. Guide Performance and Quality Tourism Service is one of part of the tourism, so the researcher focus to Guide Performance and Quality Tourism Service To Revisit Intention in Bogor Botanical Gardens. In this research, the researcher used quantitative methode with descriptive verificative. The researcher used 100 respondent for a sample. In this research, the researcher used multiple linier regresion analysis with hypotesis test used determination coefficient test and F test. The classical assumption of this research is used normality test, multicollinearity test, heteroskedastisitas test and auto correlation test. In this research, the Guide Performance and Quality Tourism Service factors has an influence 33,2% of Revisit Intention in Bogor Botanical Garden. Keywords: Guide Performance, Quality Tourism Service and Revisit Intention

FAKTOR-FAKTOR YANG MEMPENGARUHI BELANJA MODAL DI KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Rully Farel

2016-01-01

Full Text Available This study is aimed to test the effect of Gross Domestic Regional Product (PDRB Local Government Revenue (PAD, and Surplus of Budget Financing (SiLPA on the Capital Expenditure for public service in districts Bogor. The data used are time series data, namely the period 2003 - 2013. Analytical method used is double linear regression or OLS (Ordinary Least Square. The result of this research shows that either altogether or in partial, Gross Domestic Regional Product (PDRB, Local Government Revenue (PAD and Surplus of Budget Financing (SiLPA positive and significant impact on capital expenditures in the District Bogor. Variable Surplus of Budget Financing and Local Government Revenue have a considerable influence on capital expenditure. Regression models are made to explain 77.47% of the variance of capital expenditure in District BogorDOI: 10.15408/sjie.v4i2.2305

Landscape Management of Public Open Space in Bogor Heritage City

Science.gov (United States)

Pusparini, F. D.; Nurhayati; Arifin, H. S.

2017-10-01

Public open space landscape plays important role in Bogor Heritage City. Although these spaces can carry various kind of public activities and enhance environment quality, they are fragile to disturbance and changing due to city development. Therefore, as an effort to maintain public open space landscape in 8 zones within Heritage City of Bogor, management of public open space landscape is necessary. Syntesys of public open space landscape management had held by using Analytical Hierarchy Process (AHP). The AHP result showed public open space landscape in Palace and Botanical Garden of Bogor zone (20%) is in high priority to be maintained. The other zone are Situ Gede (16,9%), Plan Karsten (12,3%), European Settlement (12%), Suryakencana (11,2%), Batu Tulis (10,2%), Empang (9.2%) and West Development (8,2%). Ecological function (34,8%) becomes the most important function to be maintained (34.8%) then social culture (31.2%), economic (17%), and aesthetics (17%).

KUALITAS FISIK DAN KIMIA AIR PAM DI JAKARTA, BOGOR, TANGERANG, BEKASI TAHUN 1999 - 2001

Directory of Open Access Journals (Sweden)

Mariana Raini

2012-09-01

Full Text Available Drinking water quality of PAM in Jakarta, Bogor, Tangerang and Bekasi between the year of 1999 - 2001 has been studied. Water quality was determined by a series of physical and chemical test at Chemical Laboratory Pusat Penelitian dan Pengembangan Farmasi dan Obat Tradisional as stated in Permenkes no.416/Menkes/Per/IX/1990 on condition and water quality control including smell, color, turbidity, contents of manganese, iron, nitrite, sulphate, organic subtance, turbidity, hardness and pH. Total sampling was done on all drinking water samples from DKI Jakarta, Bogor, Tangerang and Bekasi delivered to be tested at Pusat Penelitian dan Pengembangan Farmasi dan Obat Tradisional between 1999-2001 and destilled water as control. Result of the study from 431 drinking water samples shows that samples that met the requirements as drinking water is 91,65% and 8,35% sub standard, most of the the sub standard drinking water is cause by the contents of manganese (4,41%, iron (2,09%, organic subtance (1,62%, chloride (0,93% and high water hardness (0,23% as well as pH (3,15%, turbidity (1,86% and colors (1,62% Statistically, the water quality of PAM in Jakarta, Bogor, Tangerang and Bekasi at 1999, 2000 and 2001 is not different.  Key word : water quality of PAM, drinking water, physical test, chemical test.

IMPLEMENTASI MANAJEMEN PENGETAHUAN PADA SISTEM PENGELOLAAN IJAZAH DI UPBJJ-UT BOGOR

Directory of Open Access Journals (Sweden)

Ratna Marta Dhewi

2015-04-01

Full Text Available This research aimed to examine the pattern of managing document certification at Bogor regional office for the last decade, furthermore, to design the digital model which offers insights into what is required to strategically align transcript management practice at Bogor regional office. The research was conducted by purposive sampling at Bogor Regional office considering that it represents the complexity of the organization that involves only a few employees with varying computational competencies and various task capabilities. However, they have been able to implement the knowledge management systems in providing services to customers. The data of this research were primary data. Data analysis were conducted based on an ongoing basis, with the following steps: preparation of the theme of data, classification and mapping of data based on a theme that has been set up, compilation, reduction, data cleaning, incorporation into the theme of the final data, triangulation of data, and data interpretation. This research discovered that managing certification documents of graduation at Bogor regional proved to be continuously updated and improved by the management team, and supported by top level management. The finding of this research proposed that the web based digital model of the certification documents management system (from acceptance, storage to distribution is the most suitable model to be implemented at the regional offices. Penelitian ini bertujuan mendeskripsikan pola pengelolaan dokumen sertifikasi di UPBJJ-UT Bogor selama sepuluh tahun terakhir dan menemukan model digital yang paling tepat untuk pengelolaan ijazah/transkrip yang efisien di UPBJJ-UT Bogor. Penelitian ini dilakukan dari bulan Maret sampai November 2011. Pemilihan lokasi penelitian dilakukan dengan cara purposive sampling dengan mempertimbangkan bahwa UPBJJ UT Bogor dapat mewakili kompleksitas organisasi yang melibatkan hanya beberapa pegawai dengan penguasaan komputer yang

[Equipping the bacteriological laboratories of a tuberculosis service as the most important element of assurance of the quality and safety of their work].

Science.gov (United States)

Sevast'ianova, E V; Martynova, L P; Barilo, V N; Golyshevskaia, V I; Shul'gina, M V

2009-01-01

By taking into account the present requirements for the equipping of the laboratories, the authors have drawn up the minimum standard list of equipment, as well as a list of additional equipment for the specialized bacteriological laboratory of a tuberculosis-controlling institution, which performs microbiological studies for the diagnosis and control of chemotherapy for tuberculosis. The specifications and characteristics of the baric types of equipment used to fit out the laboratories under the present conditions are described. Equipping the laboratories in accordance with the draw-up lists is shown to ensure a qualitative, effective, and safe work. Recommendations on how to supply the laboratories with equipment, to make the optimal choice, and to use consumables for tests are given.

Department of Energy multiprogram laboratories

International Nuclear Information System (INIS)

1982-09-01

The Panel recommends the following major roles and missions for the laboratories: perform the Department's national trust fundamental research missions in the physical sciences, including high energy and nuclear physics, and the radiobiological sciences including nuclear medicine; sustain scientific staff core capabilities and specialized research facilities for laboratory research purposes and for use by other Federal agencies and the private sector; perform independent scientific and technical assessment or verification studies required by the Department; and perform generic research and development where it is judged to be in the public interest or where for economic or technical reasons industry does not choose to support it. Organizational efficiencies if implemented by the Department could contribute toward optimal performance of the laboratories. The Panel recommends that a high level official, such as a Deputy Under Secretary, be appointed to serve as Chief Laboratory Executive with authority to help determine and defend the research and development budget, to allocate resources, to decide where work is to be done, and to assess periodically laboratory performance. Laboratory directors should be given substantially more flexibility to deploy resources and to initiate or adapt programs within broad guidelines provided by the Department. The panel recommends the following actions to increase the usefulness of the laboratories and to promote technology transfer to the private sector: establish user groups for all major mission programs and facilities to ensure greater relevance for Department and laboratory efforts; allow the laboratories to do more reimbursable work for others (other Federal agencies, state and local governments, and industry) by relaxing constraints on such work; implement vigorously the recently liberalized patent policy; permit and encourage joint ventures with industry

Kekerabatan rayap tanah Macrotermes gilvus Hagen (Blattodea: Termitidae dari dua habitat di Bogor

Directory of Open Access Journals (Sweden)

Nadzirum Mubin

2016-09-01

Full Text Available Subterranean termites Macrotermes gilvus (Hagen (Blattodea: Termitidae belongs to the subfamily Macrotermitinae they are distributed widely in Southeast Asia such as Indonesia, Malaysia, Singapore, Myanmar, Philippine, Vietnam, and Thailand. Many studies on these termites have been conducted, but the study of relatedness termites from different locations are barely done. The objective of this study was analyze the relatedness between subterranean termites M. gilvus in IPB Dramaga Campus and Yanlappa Nature Reserve, Jasinga-Bogor. The termite relatedness was analyzed with agonistic behavioral approach and molecularly, technique using mitochondrial COI gene. Termites from both locations showed identical molecular relatedness, however behavioral analysis show that they belong from different parental lineages. Agonistic assessment showed that individuals from different habitat showed aggressive behaviour, whereas those that originated from the same nest do not show any aggression. Molecular detection however have failed to show.

Evaluating performance in sweat testing in medical biochemistry laboratories in Croatia.

Science.gov (United States)

Aralica, Merica; Krleza, Jasna Lenicek

2017-02-15

Sweat test has a diagnostic role in evaluation of cystic fibrosis. Its performance includes sweat stimulation, collection and analysis. All listed may be sources of inconsistencies in everyday practice. The aim of this study was an evaluation of external quality assessment (EQA) of sweat chloride measurement including sweat test performance in medical biochemistry laboratories in Croatia. EQA for sweat chloride measurement was provided by Croatian Centre for Quality Assessment in Laboratory Medicine (CROQALM) in five consecutive exercises to medical biochemistry laboratories (MBL) that offered sweat testing. A questionnaire regarding all phases of testing was mailed to involved MBL (N = 10). Survey results were compared to current guidelines for sweat test performance. Reported results of EQA in 2015 exercises showed coefficients of variation (CV) from 28.9%, 29.0% to 35.3%, respectively. An introduction of uniform sweat chloride measurement protocol resulted in CV of 15.5% and 14.7% reported in following two exercises in 2016. All MBL included in this study replied to the questionnaire. Results reported by MBL indicated: lack of patient information policy (7/10), use of unacceptable electrodes (6/9), misuse of minimum of acceptable sweat weight (6/9), lack of internal quality assessment (5/9) and recommended reference ranges (5/9 and 4/9). Agreements to guidelines were found in approach to unsuitable patients (9/10) and sweat collection (8/9). Presented results indicate major weak points of current practice in sweat test performance in Croatian MBL and stress the need for its standardization on a national level.

Purification and Characterization of Taq Polymerase: A 9-Week Biochemistry Laboratory Project for Undergraduate Students

Science.gov (United States)

Bellin, Robert M.; Bruno, Mary K.; Farrow, Melissa A.

2010-01-01

We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of "Thermus aquaticus" DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having each week's procedure focus on a single…

Biochemistry Instrumentation Core Technology Center

Data.gov (United States)

Federal Laboratory Consortium — The UCLA-DOE Biochemistry Instrumentation Core Facility provides the UCLA biochemistry community with easy access to sophisticated instrumentation for a wide variety...

Tingkat Infestasi Aphelenchoides besseyi pada Benih Padi di Bogor

Directory of Open Access Journals (Sweden)

Fitrianingrum Kurniawati

2016-03-01

Full Text Available The white tip disease on rice plants have been discovered in the district of Bogor, i.e. in  Sukamakmur, Petir, and Muara Experimental Station areas. The infected plant exhibited symptoms, involving whiten leaf tip 3–5 cm that later  turn to necrosis, twisted and crinkled. The disease is supposed caused by a parasitic nematode, namely Aphelenchoides besseyi, which is known seed borne. Extraction of nematodes based on  ISTA protocols on rice was conducted.  Seeds from eight varieties, SL8SHS, Hybrid Rice (HIPA14, IPB 3S, IR-64, Pertiwi (Pak Tiwi, Inpari 31, Pandan Wangi Bogor (Sintanur and Ciherang, was obtained from seed vendors in Bogor and Muara Experimental Station. The majority of nematodes recovered were females and only few males with morphological characteristics matching with A. besseyi.  All seed rice varieties tested contained A. besseyi with the average of 3–341 nematodes per 5 g seed. 

KEBIASAAN DAN KUALITAS SARAPAN PADA SISWI REMAJA DI KABUPATEN BOGOR

OpenAIRE

Anna Febritta Intan Sari; Dodik Briawan; Cesilia Meti Dwiriani

2013-01-01

ABSTRACTThe study aimed to describe breakfast habit among teenage girls of vocational high school Bogor. A cross-sectional study was conducted and was involving 68 students 14—18 years old from SMK Pelita Ciampea, Bogor District. Breakfast consumption was collected by a 7-day food records. The result showed that there were 45.6% schoolgirls having breakfast regularly. Breakfast frequency per week was associated with BMI and anaemic status (p

KEBIJAKAN HUKUM PIDANA DALAM MENANGGULANGI TINDAK PIDANA PERTAMBANGAN TENTANG MENDULANG EMAS SECARA TRADISIONAL YANG MENGAKIBATKAN PENCEMARAN DAN MENELAN KORBAN JIWA BERDASARKAN UNDANG-UNDANG NOMOR 4 TAHUN 2009 TENTANG PERTAMBANGAN MINERAL DAN BATU BARA (STUDI KASUS GUNUNG PONGKOR BOGOR)

OpenAIRE

Margo Hadi Pura, S.H., M.H.

2016-01-01

State of Indonesia is one country in the world that has natural resources that are very much whether it is renewable and can not be renewed (or unerewable). Examples of non-renewable wealth of the mining sector, among others, one of them a gold mine. Not all parts of Indonesia has a potential gold mine that has one gold mine is in the western part of Mount Pongkor Kab. Bogor, West Java. Until now, the hunt for gurandil (designation illegal gold miners) and traditional gold mining in this regi...

KONSUMSI BUAH DAN SAYUR ANAK USIA SEKOLAH DASAR DI BOGOR

Directory of Open Access Journals (Sweden)

Andika Mohammad

2015-05-01

Full Text Available ABSTRACTThe aim of this study was to analyze factors related to fruits and vegetables consumption of elementary school children in Bogor. The design of this study was cross-sectional with purposive sampling. A total of 108 children in SDN Cibanteng 01 in rural area and SDN Papandayan in urban area. Children consumption of fruits and vegetables in SDN Papandayan was higher than SDN Cibanteng. There were significant differences between children consumption of fruits based on ethnic and mother`s job (p<0.05. Children knowledge of nutrition and education of father and mother significantly positive associated with children consumption of fruits and vegetables, as well as pocket money, availability of fruits in house, and family income positive significantly associated with children consumption of fruits (p<0.05.Keywords: children, consumption, fruits and vegetables, socio-economicABSTRAKTujuan penelitian ini adalah menganalisis faktor-faktor yang berhubungan dengan konsumsi buah dan sayur pada anak usia sekolah dasar di Bogor. Desain penelitian ini adalah cross sectional dengan teknik penarikan subjek secara purposive sebanyak 108 subjek di dua sekolah dasar di Bogor yaitu SDN Cibanteng 01 Kabupaten Bogor dan SDN Papandayan Kota Bogor. Konsumsi buah dan sayur di SDN Papandayan lebih besar dibandingkan dengan SDN Cibanteng. Terdapat perbedaan yang nyata konsumsi buah anak berdasarkan suku dan pekerjaan ibu (p<0,05. Pengetahuan gizi anak dan pendidikan ayah dan ibu berhubungan signifikan positif dengan konsumsi buah dan sayur anak, demikian pula uang saku, ketersediaan buah, dan pendapatan keluarga berhubungan signifikan positif dengan konsumsi buah anak (p<0,05.Kata kunci: anak, buah dan sayur, konsumsi, sosial-ekonomi

Prepare, Do, Review: A skills-based approach for laboratory practical classes in biochemistry and molecular biology.

Science.gov (United States)

Arthur, Peter; Ludwig, Martha; Castelli, Joane; Kirkwood, Paul; Attwood, Paul

2016-05-06

A new laboratory practical system is described which is comprised of a number of laboratory practical modules, each based around a particular technique or set of techniques, related to the theory part of the course but not designed to be dependent on it. Each module comprises an online recorded pre-lab lecture, the laboratory practical itself and a post-lab session in which students make oral presentations on different aspects of the practical. Each part of the module is assessed with the aim of providing rapid feedback to staff and students. Each laboratory practical is the responsibility of a single staff member and through this "ownership," continual review and updating is promoted. Examples of changes made by staff to modules as a result of student feedback are detailed. A survey of students who had experienced both the old-style laboratory course and the new one provided evidence of increased satisfaction with the new program. The assessment of acquired shills in the new program showed that it was much more effective than the old course. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:276-287, 2016. © 2016 The International Union of Biochemistry and Molecular Biology.

Culturing Security System of Chemical Laboratory in Indonesia

OpenAIRE

Pusfitasari, Eka Dian

2017-01-01

Indonesia has experiences on the lack of chemical security such as: a number of bombing terrors and hazardous chemicals found in food. Bomb used in terror is a homemade bomb made from chemicals which are widely spread in the research laboratories such as a mixture of pottasium chlorate, sulphur, and alumunium. Therefore, security of chemicals should be implemented to avoid the misused of the chemicals. Although it has experienced many cases of the misuse of chemicals, and many regulations and...

Teaching biochemistry to medical students in Singapore--from organic chemistry to problem-based learning.

Science.gov (United States)

Khoo, H E

2005-07-01

The medical faculty in the National University of Singapore started in 1905 but the Chair in Biochemistry was only established in 1927. For many years the biochemistry course consisted of the teaching of the organic chemistry of substances of physiological importance, nutrition, metabolism and hormones. In 1961, clinical biochemistry was introduced and in the 1980s, genetics and molecular biology were included. By then, most of the organic chemistry content had been removed as greater emphasis was placed on clinical correlation. Laboratory classes consisted of mock glucose tolerance tests and the measurement of various enzymes. By the 1990s, students were no longer interested in such practical classes, so a bold decision was made around 1995 to remove laboratory classes from the curriculum. Unfortunately, this meant that the medical students who might have been interested in laboratory work could no longer do such work. However, the new curriculum in 1999 gave the department an opportunity to offer a laboratory course as an elective for interested students. This new curriculum adopted an integrated approach with Genetics being taught as part of Paediatrics, and a new module (Structural and Cell Biology) comprising aspects of cell biology and biochemistry was introduced. This module is currently taught by staff from Anatomy, Physiology and Biochemistry. Some biochemistry content is now incorporated into the clinical problem scenarios of problem-based learning such as jaundice, diabetes mellitus, anorexia nervosa, etc. So the evolution of teaching biochemistry to medical students in Singapore has paralleled worldwide trends and moved from the didactic teaching of organic chemistry of biomolecules to problem-based learning using clinical cases.

Biochemistry Facility

Data.gov (United States)

Federal Laboratory Consortium — The Biochemistry Facility provides expert services and consultation in biochemical enzyme assays and protein purification. The facility currently features 1) Liquid...

Litter Decomposition Rate of Karst Ecosystem at Gunung Cibodas, Ciampea Bogor Indonesia

Directory of Open Access Journals (Sweden)

Sethyo Vieni Sari

2016-05-01

Full Text Available The study aims to know the productivity of litter and litter decomposition rate in karst ecosystem. This study was conducted on three altitude of 200 meter above sea level (masl, 250 masl and 300 masl in karst ecosystem at Gunung Cibodas, Ciampea, Bogor. Litter productivity measurement performed using litter-trap method and litter-bag method was used to know the rate of decomposition. Litter productivity measurement results showed that the highest total of litter productivity measurement results was on altitude of 200 masl (90.452 tons/ha/year and the lowest was on altitude of 300 masl (25.440 tons/ha/year. The litter productivity of leaves (81.425 ton/ha/year showed the highest result than twigs (16.839 ton/ha/year, as well as flowers and fruits (27.839 ton/ha/year. The rate of decomposition was influenced by rainfall. The decomposition rate and the decrease of litter dry weight on altitude of 250 masl was faster than on the altitude of 200 masl and 300 masl. The dry weight was positively correlated to the rate of decomposition. The lower of dry weight would affect the rate of decomposition become slower. The average of litter C/N ratio were ranged from 28.024%--28.716% and categorized as moderate (>25. The finding indicate that the rate of decomposition in karst ecosystem at Gunung Cibodas was slow and based on C/N ratio of litter showed the mineralization process was also slow.

Development of a Semester-Long, Inquiry-Based Laboratory Course in Upper-Level Biochemistry and Molecular Biology

Science.gov (United States)

Murthy, Pushpalatha P. N.; Thompson, Martin; Hungwe, Kedmon

2014-01-01

A semester-long laboratory course was designed and implemented to familiarize students with modern biochemistry and molecular biology techniques. The designed format involved active student participation, evaluation of data, and critical thinking, and guided students to become independent researchers. The first part of the course focused on…

Keragaman Jenis dan Prevalensi Lalat Pasar Tradisional di Kota Bogor (DIVERSITY AND PREVALENCE OF FLIES AT TRADITIONAL MARKETS IN BOGOR CITY

Directory of Open Access Journals (Sweden)

Puguh Wahyudi

2016-01-01

Full Text Available Bogor city is one of the greater Jabodetabek area which has a fairly high growth of the modern market.This should not shift the role of traditional market, if accompanied with an increase in the number andquality of traditional markets, among others by controlling infestations of flies on the market that can bea vector of various diseases.This research was conducted to identify the diversity and infestation of fliesspesies in five old Bogor traditional markets. The flies were collected using insect nets and then killed withchloroform to count and identification purposes. Measuring the prevalence of flies infestation in eachmarket were using sticky fly paper on block sale of meat, fish and outside market environment. Therewere ten fly spesies belong to four main families that Calliphoridae (C. megacephala, C. saffranea, C.rufifacies, and Lucilia sericata, Muscidae (M. domestica, M. conducens, and M. fasciata, Sarcophagidae (S.haemorroidalis, and S. fuscicauda, and Drosophilidae (Drosophila repleta. The others three families werePhoridae, Anthomyiidae, and Syrphidae. Fly diversity index on each markets were 1.203 (Bogor Market,1.038 (Sukasari Market, 2.678 (Anyar Market, 1.017 (Jambu Dua Market, and 1.618 (Gunung BatuMarket. Measurement of Calliphorid flies infestations as an indicator of the presence of litterdecomposition of organic material showed a high concentration in the market environment.These resultsillustrate the general environmental sanitation of traditional markets are bad.

BIAYA TRANSAKSI DAN NILAI TAMBAH PADA RANTAI PASOK DAGING SAPI DI KOTA BOGOR

Directory of Open Access Journals (Sweden)

Nadya Megawati Rachman

2017-03-01

Full Text Available The objectives of this study are to map the distribution network of beef supply chain in Bogor city and analyze the transaction cost, value added and marketing efficiency in the marketing channel of beef supply chain in Bogor city. Value stream mapping (VSM was used to analyze the distribution channel of beef supply chain. Hayami method was used to analyze transaction cost, value added and marketing efficiency. The result shows that there are 9 alternative distribution channels of beef marketing in Bogor city. The biggest value added gained by PBDS I (22.24% is from slaughtering activities. Transaction cost in supply chain process is only 3–5 % the total costs. The dominating cost is the cost to buy beef supply (60%, and the most efficient and profitable channel is channel 7 (Feedloter – PBDS I – Consumer with the marketing efficiency score of 0.80%. Therefore, the butchers in Bogor city are recommended to choose channel 7 for its highest benefit and efficient channel. Keywords: beef, supply chain, transaction cost, value added, hayami methodAbstrakTujuan dari penelitian ini adalah memetakan jaringan distribusi rantai pasok daging sapi di Kota Bogor dan menganalisis biaya transaksi, nilai tambah dan tingkat efisiensi pemasaran dari saluran pemasaran daging sapi di Kota Bogor. Value stream mapping (VSM digunakan untuk memetakan jaringan distribusi daging sapi  dan metode hayami digunakan untuk menganalisis biaya transaksi, nilai tambah dan efisiensi pemasaran. Berdasarkan hasil pemetaan jaringan distribusi rantai pasok daging sapi dengan menggunakan VSM terdapat sembilan alternatif saluran distribusi pemasaran daging sapi di Kota Bogor. Nilai tambah terbesar diperoleh dari hasil pemotongan sapi hidup menjadi karkas yang didapatkan oleh PBDS I (22,24%. Biaya transaksi dalam proses pasokan jaringan distribusi hanya berkisar 3–5% dari total biaya yang dikeluarkan. Biaya yang mendominasi adalah biaya dalam membeli pasokan daging sapi yang

Teaching Protein Purification and Characterization Techniques: A Student-Initiated, Project-Oriented Biochemistry Laboratory Course

Science.gov (United States)

MacDonald, Gina

2008-01-01

This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, and made buffers they continue to learn basic protein purification and biochemical techniques…

KARAKTERISTIK KLIEN YANG DIRAWAT DI RUANG MODEL PRAKTEK KEPERAWATAN PROFESIONAL RUMAH SAKIT Dr. H. MARZOEKI MAHDI BOGOR

Directory of Open Access Journals (Sweden)

Ice Yulia Wardani

2003-06-01

Full Text Available Since September 2000, Community and Mental Health Department Faculty of Nursing University of Indonesia, have developed Professional Nursing Practice Model (PNPM on Psychiatric Nursing at Dr. H. Marzoeki Mahdi Health Hospital. PNPM at Marzoeki Mahdi Hospital is the first PNPM especially for mental health. The study was a quantitative cross sectional study conducted during the period February 4th to July 30th 2001 and covering 79 client. The aim of the study was to identify characteristics of clients at PNPM. It was found that most of the clients were males, aged 20-55 years, living in the area of Jabotabek (Jakarta, Bogor, Tangerang and Bekasi, especially in Bogor, the city of the hospital. Most of them had no job, with a senior high school education whereas the number with unmarried status was nearly the same with the number with married status and belonged to the Sundanese ethnic group. Characteristics of the family were mostly as followed: big family, with more than 2 children, closed communication and family meeting during meals. On health status it was found that clients were admitted to the hospital because of angry/violence, angry/violence and talking/laughing by themselves, whereas the number of clients in the group never nursed in a hospital before were nearly the same as the number in the group ever nursed before, with average length of staying in hospital 9 days. It was revealed that the problem of hallucination was mostly found among the nursing problems. Health education was mostly on efforts to eliminate paranoid problems and Activity Group Therapy was focused on efforts to socialize the clients. The medical diagnosis of most of the clients was paranoid schizophrenia. It was concluded that in this study characteristics of the clients in PNPM was very varied. For optimal nursing criteria of clients at PNPM who should be admitted should be established.

Bring Your Own Device: A Digital Notebook for Undergraduate Biochemistry Laboratory Using a Free, Cross-Platform Application

Science.gov (United States)

Van Dyke, Aaron R.; Smith-Carpenter, Jillian

2017-01-01

The majority of undergraduates own a smartphone, yet fewer than half view it as a valuable learning technology. Consequently, a digital laboratory notebook (DLN) was developed for an upper-division undergraduate biochemistry laboratory course using the free mobile application Evernote. The cloud-based DLN capitalized on the unique features of…

KANDUNGAN VITAMIN B6, B9, B12 DAN E BEBERAPA JENIS DAGING, TELUR, IKAN DAN UDANG LAUT DI BOGOR DAN SEKITARNYA (VITAMIN B6, B9, B12 AND E CONTENT OF SEVERAL TYPES OF MEATS, EGGS, FISHES AND MARINE SHRIMPS IN BOGOR AND SURROUNDING AREAS

Directory of Open Access Journals (Sweden)

Heru Yuniati

2012-06-01

Full Text Available ABSTRACT Food Composition Table (DKBM in Indonesia has not mentioned all types of nutrients available in the food, particularly vitamin B6, B9 (folic acid, B12, and vitamin E. Therefore this study aimed to analyze the content of vitamin B6, B9 (folic acid, B12, and vitamin E in several types of meat, eggs, fish and marine shrimps consumed in Bogor and surrounding areas. Vitamin B6, B9, B12, and vitamin E from three kinds of meat (chicken, beef, lamb, two types of eggs (chicken, duck, and four species of fish (snapper, bloating, carp and tuna and crayfish are analyzed using High-Performance Liquid Chromatography (HPLC. The samples used are raw and taken from three locations in Bogor and surrounding areas. Fishes, meats and eggs contain high levels of folic acid, however the amount of folic acid content in meat varies depending on which part of meat the samples are taken, types of organ, and the fat content of the meat. The folic acid content in chicken wings is different with those in thigh. In fatty mutton the folic acid is higher than in those lean meat, and in yolk is higher than those in egg white. Vitamin E content of snapper is the highest amongs other types of fishes (6.54 µg/100 g.Chicken eggs contain a higher amount of vitamin E than duck eggs, while the yolk contains ahigher amount of vitamin E than those egg white. Keywords: animal foods, vitamin B6, vitamin B9 (folic Acid, vitamin B12, vitamin E   ABSTRAK Daftar Komposisi Bahan Makanan (DKBM yang ada di Indonesia belum memuat semua jenis zat gizi dalam makanan, khususnya vitamin B6, B9 (asam folat, B12 dan vitamin E. Menganalisis kandungan vitamin B6, B9 (asam folat, B12, dan vitamin E dalam beberapa jenis daging, telur, ikan dan udang laut yang dikonsumsi masyarakat di Bogor dan sekitarnya. Kandungan vitamin B6, B9, B12 dan vitamin E dari tiga jenis daging (ayam, sapi, kambing, dua jenis telur (ayam, itik, serta empat jenis ikan (kakap, kembung, mas, tongkol dan udang laut

[Outsourcing of clinical laboratory department].

Science.gov (United States)

Murai, T

2000-03-01

Recently, to improve financial difficulties at various hospitals, outsourcing of the laboratory department is be coming more wide spread. At the department of clinical pathology of St. Luke's International Hospital, the system, so called, "Branch labo" which is one of the outsourcing laboratory conditions, was adopted in March 1999. In this reports. We described the decision procedure for accepting the situation and the circumstances of operation.

Establishing a laboratory network of influenza diagnosis in Indonesia: an experience from the avian flu (H5N1 outbreak

Directory of Open Access Journals (Sweden)

Setiawaty V

2012-08-01

Full Text Available Vivi Setiawaty, Krisna NA Pangesti, Ondri D SampurnoNational Institute of Health Research and Development, Ministry of Health, the Republic of Indonesia, Jakarta, IndonesiaAbstract: Indonesia has been part of the global influenza surveillance since the establishment of a National Influenza Center (NIC at the National Institute of Health Research and Development (NIHRD by the Indonesian Ministry of Health in 1975. When the outbreak of avian influenza A (H5N1 occurred, the NIC and US Naval Medical Research Unit 2 were the only diagnostic laboratories equipped for etiology confirmation. The large geographical area of the Republic of Indonesia poses a real challenge to provide prompt and accurate diagnosis nationally. This was the main reason to establish a laboratory network for H5N1 diagnosis in Indonesia. Currently, 44 laboratories have been included in the network capable of performing polymerase chain reaction testing for influenza A. Diagnostic equipment and standard procedures of biosafety and biosecurity of handling specimens have been adopted largely from World Health Organization recommendations.Keywords: influenza, laboratory, networking

Team-Based Learning, Faculty Research, and Grant Writing Bring Significant Learning Experiences to an Undergraduate Biochemistry Laboratory Course

Science.gov (United States)

Evans, Hedeel Guy; Heyl, Deborah L.; Liggit, Peggy

2016-01-01

This biochemistry laboratory course was designed to provide significant learning experiences to expose students to different ways of succeeding as scientists in academia and foster development and improvement of their potential and competency as the next generation of investigators. To meet these goals, the laboratory course employs three…

9 CFR 147.11 - Laboratory procedure recommended for the bacteriological examination of salmonella.

Science.gov (United States)

2010-01-01

... procedure recommended for the bacteriological examination of salmonella. (a) For egg- and meat-type chickens, turkeys, waterfowl, exhibition poultry, and game birds. All reactors to the pullorum-typhoid tests, up to...

HPLC of the Polypeptides in a Hydrolyzate of Egg-White Lysozyme. An Experiment for the Undergraduate Biochemistry Laboratory.

Science.gov (United States)

Richardson, W. S., III; Burns, L.

1988-01-01

Describes a simple high-performance liquid chromatography experiment for undergraduate biochemistry laboratories. The experiment illustrates the separation of polypeptides by a step gradient elution using a single pump instrument with no gradient attachments. Discusses instrumentation, analysis, a sample preparation, and results. (CW)

Identification of Forensic Samples via Mitochondrial DNA in the Undergraduate Biochemistry Laboratory

Science.gov (United States)

Millard, Julie T.; Pilon, André M.

2003-04-01

A recent forensic approach for identification of unknown biological samples is mitochondrial DNA (mtDNA) sequencing. We describe a laboratory exercise suitable for an undergraduate biochemistry course in which the polymerase chain reaction is used to amplify a 440 base pair hypervariable region of human mtDNA from a variety of "crime scene" samples (e.g., teeth, hair, nails, cigarettes, envelope flaps, toothbrushes, and chewing gum). Amplification is verified via agarose gel electrophoresis and then samples are subjected to cycle sequencing. Sequence alignments are made via the program CLUSTAL W, allowing students to compare samples and solve the "crime."

DETERMINAN PEMBERIAN MAKANAN PRELAKTAL PADA BAYI BARU LAHIR DI KELURAHAN KEBON KELAPA DAN CIWARINGIN, KOTA BOGOR (DETERMINANTS OF PRELACTEAL FEEDING AMONG NEWBORN BABIES IN KEBON KELAPA AND CIWARINGIN VILLAGES, BOGOR

Directory of Open Access Journals (Sweden)

Bunga Ch Rosha

2013-06-01

Full Text Available ABSTRACT Breast milk is the appropriate for babies for the first six months after birth. Although it has been known that exclusive breastfeeding has many benefits and encouraged by the government, but the proportion of exclusive breasfeeding is still low, including Bogor. One of the the reason for the failure in exclusive breastfeeding is the prelacteal feeding practice among newborn babies. This analysis was conducted to provide information on the determinants of prelacteal feeding practice in Kebon Kelapa and Ciwaringin Village in Bogor, in 2012. The data for this analysis was came from the child growth and development cohort study conducted in Kebon Kelapa and Ciwaringin, Bogor in 2012. Participants of the study were 91 mothers with her newborn babies. The data were analysed with descriptive analysis, bivariate analysis with chi square, and multivariate analysis using logistic regression. The results showed that mode of delivery, rooming-in care and time of breastfeeding after delivery were significantly associated with prelacteal feeding practice (p<0,05. The major determinant factor of prelacteal feeding practice was non rooming-in care (OR: 5.86; 95% CI: 1.17, 29.35 after controlling the time of breastfeeding after delivery. Postpartum mothers that not cared in the same room with the baby had risk 5.86 times for give the baby prelacteal food compared with postpartum mothers that cared in the same room with their baby. Mothers who breastfeed their baby more than 1 hour after delivery had risk of 4.87 times for give the baby prelacteal food compared with mothers who breastfeed less than 1 hour after delivery. Therefore, it is necessary to improve the implementation of Baby Friendly Hospitals program in maternal and child health services, especially the implementation of rooming-in care for mother and child, so that the mother can breastfeed immediately after birth so that the baby does not need to be given food or prelacteal liquids. The

Pengaruh Kemitraan terhadap Produksi dan Pendapatan USAhatani Sayuran di Kabupaten Bogor

OpenAIRE

Susanti, Susanti; Kusnadi, Nunung; Rachmina, Dwi

2014-01-01

The partnership between Gapoktan Rukun Tani and vegetable farmers in Bogor was designed to facilitate farmers in overcoming the limitations of production inputs, capital, and market certainty. This study compares a one year production and income of vegetables farmers who participate in the partnership to the non participated vegetables farmers in Bogor. Income analysis and R/C ratio analysis show that the partnership has no influence in increasing the farm production and income. In term of a...

A Project-Based Biochemistry Laboratory Promoting the Understanding and Uses of Fluorescence Spectroscopy in the Study of Biomolecular Structures and Interactions

Science.gov (United States)

Briese, Nicholas; Jakubowsk, Henry V.

2007-01-01

A laboratory project for a first semester biochemistry course is described, which integrates the traditional classroom study of the structure and function of biomolecules with the laboratory study of these molecules using fluorescence spectroscopy. Students are assigned a specific question addressing the stability/function of lipids, proteins, or…

Must we review printed lab reports without checking them? A prospective analysis of emergency department practice.

LENUS (Irish Health Repository)

McCabe, A

2012-04-01

This study investigated if results of haematology and biochemistry laboratory tests, carried out at the point of care in our Emergency Department, are checked by the clinician who ordered the test, mitigating the requirement to check printed reports later. Five hundred and nineteen (519) laboratory reports were examined for significant abnormal results and documentation in clinical notes. Thirty percent (30%, n = 158) of these met the inclusion criteria for \\'significantly abnormal\\' laboratory results. Of the 158 significantly abnormal results, 34.8% (n = 55) were not documented in the ED clinical case notes. No patient was discharged inappropriately. Our study suggests it is safe to stop routinely rechecking printed biochemistry and haematology laboratory reports in our department.

MODEL SIMULASI EMISI DAN PENYERAPAN CO2 DI KOTA BOGOR

Directory of Open Access Journals (Sweden)

Rizka Permatayakti Rasyidta Nur

2015-04-01

Full Text Available Most of the urban pollution is the result of carbon dioxide (CO2 emission from human activities. This research identified CO2 emission and absorption in Bogor, and also the alternatives to solve the emission problem by system model and simulation. CO2 emission and absorption system model was created using software Stella 9.0.2 based on loss-gain emission concept for 30 years prediction. Human activities that contribute to CO2 emission are transportation, industries, energy consumption such as fuel or electricity, house hold waste, and farms, while the decrease factor is green open spaces as CO2 sequester. The alternatives to solve emission problem in Bogor is created based on green city concept by including the environmental aspects in every urban activity. The result of this research, the CO2 emission of Bogor reached 20.027.878 tons and the absorption reached 93.843 tons in 2042. Combined mitigation alternatives in several sectors could reduce CO2 emission by 2.797.667 tons in 2042 and CO2 emission could be neutralized by reforestation in 2036.

Climate Risk Management and Decision Support Tools for the Agriculture Sector in Lao PDR, Bangladesh, and Indonesia

Science.gov (United States)

Allis, E. C.; Greene, A. M.; Cousin, R.

2014-12-01

We describe a comprehensive project for developing climate information and decision support / climate risk management tools in Lao PDR, Bangladesh and Indonesia. Mechanisms are developed for bringing the benefits of these tools to both policy makers and poor rural farmers, with the goal of enabling better management, at the farm level, of the risks associated with climate variability and change. The project comprises several interwoven threads, differentially applied in the different study regions. These include data management and quality control, development of seasonal forecast capabilities, use of dynamic cropping calendars and climate advisories, the development of longer-term climate information for both past and future and a weather index insurance component. Stakeholder engagement and capacity building served as reinforcing and complementary elements to all components. In this talk we will provide a project overview, show how the various components fit together and describe some lessons learned in this attempt to promote the uptake of actionable climate information from farmer to policy level. The applied research project was led by the International Research Institute for Climate and Society (IRI) at Columbia University with funding from the International Fund for Agriculture Development (IFAD) and in close collaboration with our regional partners at the Centre for Climate Risk and Opportunity Management in Southeast Asia Pacific (at Bogor Agricultural University in Indonesia), Indonesia's National Agency for Meteorology, Climatology and Geophysics (BMKG), Lao PDR's National Agriculture and Forestry Research Institute (NAFRI), Laotian Department of Meteorology and Hydrology (DMH), WorldFish Center, Bangladesh Meteorology Department (BMD), and CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS).

PEMILIHAN LOKASI TERBAIK PENGEMBANGAN PROPERTI APARTEMEN DI PERKOTAAN CIBINONG RAYA KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Muhammad Saifuddin Amanullah

2017-07-01

Full Text Available As a part of Greater DKI Jakarta or known as Jabodetabek, Cibinong Raya offers alternatives for residence location. The statistic of population in Cibinong noted that its population has grown by 16.37% (BPS Bogor Regency, 2016 that has implication on the increase of residential dwelling demand. However, the amount of non-built area in the city is limited. So, land use optimazition strategy is significant to reach the optimum land-use value. The result of this study is expected to give different perpective of property development applied by the property developers in Indonesia. Hence, this research applied variables derived from the literature reviews and asked the property experts to analyse the variables. Using Analytical Hierarchy Process (AHP, it was revealed that there were four suitable alternative location named as Location A, Location B, Location C and Location D. Based on the experts perception through AHP method, the study showed that the highest optimum value was Location D (0.336 and followed by Location A (0.309, Location C (0.218 and Location B (0.139.

Analisis Kualitas Pelayanan di Restoran Saung Mirah, Bogor

Directory of Open Access Journals (Sweden)

Agung Gita Subakti

2014-05-01

Full Text Available A restaurant is one of the facilities to carry out Food Service Industry or as a part tourism accommodation that plays a role in fulfilling the needs of tourists or customer. In this case, good service quality will determine the progress of a restaurant beside food and beverage as the offered products. Saung Mirah Restaurant Bogor is a restaurant that has a quality of service that is not as expected, thus causing a lot of complaints from guests. The purpose of this study is to look at the implementation of quality of service performed and what improvements could be given to the management of Saung Mirah Restaurant Bogor, so the quality of the restaurant service can be increased and complaints from customers can be decreased.

Taking a new biomarker into routine use – A perspective from the routine clinical biochemistry laboratory

Science.gov (United States)

Sturgeon, Catharine; Hill, Robert; Hortin, Glen L; Thompson, Douglas

2010-01-01

There is increasing pressure to provide cost-effective healthcare based on “best practice.” Consequently, new biomarkers are only likely to be introduced into routine clinical biochemistry departments if they are supported by a strong evidence base and if the results will improve patient management and outcome. This requires convincing evidence of the benefits of introducing the new test, ideally reflected in fewer hospital admissions, fewer additional investigations and/or fewer clinic visits. Carefully designed audit and cost-benefit studies in relevant patient groups must demonstrate that introducing the biomarker delivers an improved and more effective clinical pathway. From the laboratory perspective, pre-analytical requirements must be thoroughly investigated at an early stage. Good stability of the biomarker in relevant physiological matrices is essential to avoid the need for special processing. Absence of specific timing requirements for sampling and knowledge of the effect of medications that might be used to treat the patients in whom the biomarker will be measured is also highly desirable. Analytically, automation is essential in modern high-throughput clinical laboratories. Assays must therefore be robust, fulfilling standard requirements for linearity on dilution, precision and reproducibility, both within- and between-run. Provision of measurements by a limited number of specialized reference laboratories may be most appropriate, especially when a new biomarker is first introduced into routine practice. PMID:21137030

Business analytics of specialized medical biochemistry laboratory using profit and loss acount

Directory of Open Access Journals (Sweden)

Vikica Buljanović

2011-07-01

Full Text Available Introduction. By measuring the actual effectiveness of a medical biochemistry laboratory’s business operations, we can determine the accounting measure of laboratory’s profitability, where operating expenses of the laboratory are covered by the income generated from the services. A laboratory’s financial report can be based on a profit and loss account, which shows whether or not a business entity, i.e., the laboratory, is making a profit during a particular business period. Methods. Profitability of the Specialized Medical Biochemical Laboratory (Laboratory of the General County Hospital in Našice, Croatia, was determined using the profit and loss account for 2007. Business success was expressed using the accounting measures of marginal contribution, gross income, and operating income, which could show whether or not the laboratory was operating profitably. This procedure allowed us to identify indicators of successful or unsuccessful business operations of the Laboratory. Results. According to the profit and loss account, the operating profit was 719,926 HRK, i.e., the operating margin was 11.7%, indicating that the Laboratory was operating positively. After subtracting all operating expenses per 100 income units, 11.7 units profit remained from the Laboratory’s core business. Conclusion. The Specialized Medical Biochemical Laboratory of the General County Hospital in Našice generated income, i.e., it operated at a profit. The purpose of profit and loss account was to determine the Laboratory operations that had impact on its business effectiveness and could increase the actual profitability. If the laboratory operates at a loss, and no activities are undertaken that would reverse the business toward positive, the analysis may provide information on the cost for the society as a whole of the studied laboratory within the existing healthcare system.

Peran Intervensi Gizi Spesifik dan Sensitif dalam Perbaikan Masalah Gizi Balita di Kota Bogor

Directory of Open Access Journals (Sweden)

Bunga Ch Rosha

2016-09-01

Full Text Available AbstractIndonesia is facing double burden of malnutrition among children under-five. To resolve the problem, National Movement on Acceleration of Nutrition Improvement Program was conducted in the form of First Thousand Days of Life (1000 HPK through specific and sensitive nutritional interventions performed by the health and and non-health sectors. The aim of the study was to identify specific and sensitive interventions that have been done to resolve the nutritional problems of under-five children in Bogor city. This was a qualitative study on 12 informants from the health and nonhealth sector. Data were collected through indepth interviews. The results showed that specific nutritional interventions were for the child (monitoring of Posyandu, immunization, vitamin A, and supplementary foods, for mother (pregnancy class, supplementary foods for pregnant women, nutrition and health seminars and for adolescents (iron supplementation program. Whereas the sensitive nutrition interventions were environmental health interventions (Friday or Sunday cleaning program, making biopori, constructing communal septic tank, poverty interventions (cash transfer program, family hope program and PNPM, and women’s empowerment interventions (education of health and nutrition, provision of seedlings for environment utilization. In conclusion, the specific and sensitive interventions should be integrated so the handling of the nutrition problems could be carried out sustainably.Keywords : overcoming the nutrition problem, specific nutrition interventions, sensitive nutrition intervention.AbstrakIndonesia mengalami permasalahan gizi ganda pada anak balita. Untuk mengatasi masalah ini, dilakukan Gerakan Nasional Percepatan Perbaikan Gizi dalam rangka Seribu Hari Pertama Kehidupan (1000 HPK melalui intervensi gizi spesifik dan sensitif baik oleh sektor kesehatan dan non–kesehatan. Penelitian ini bertujuan mengidentifikasi intervensi spesifik dan sensitif apa saja

Diagnostic virology laboratory within a microbiology setting.

OpenAIRE

Rubin, S. J.

1984-01-01

The virology section at St. Francis Hospital and Medical Center, Connecticut, is not a separate laboratory division but is a part of the microbiology division and is supervised by the same personnel who supervise bacteriology, mycology, mycobacteriology, and serology. Current volume is over 1,000 cultures yearly with 12 to 24 percent positive. Isolates are confirmed and typed by the Connecticut State Health Department Laboratory. Specimen distribution, percentage positive specimens, and distr...

MEASURING GROCERY STORES SERVICE QUALITY IN INDONESIA: A RETAIL SERVICE QUALITY SCALE APPROACH

Directory of Open Access Journals (Sweden)

Leonnard Leonnard

2017-12-01

Full Text Available The growing number of modern grocery stores in Indonesia is a challenge for each grocery store to maintain and increase their number of consumers. The success of maintaining and improving service quality will affect long-term profitability and business sustainability. Therefore, in this study, we examined consumer perceptions of service quality in one of modern grocery stores in Indonesia. Data were collected from 387 consumers of grocery stores in Jakarta, Bogor, Depok, Bekasi, Cibubur, and Subang. Structural Equation Modeling (SEM through Maximum likelihood and Bayesian estimation was employed to analyze the data. The finding indicated that the five indicators of the retail service quality scale consisting of physical aspects, reliability, personal interactions, problem solving and policies provided  valid multi-item instruments in measuring consumer perceptions of service quality in grocery stores.

Strengthening Laboratory Capacity for Early Warning Alert and Response System (EWARS) in Indonesia

OpenAIRE

Kandel, Nirmal; Hapsari, Ratna Budi; Riana, Dyah Armi; Setiawaty, Vivi; Larasati, Wita; Wulandari, Endang; Purwanto, Edy

2017-01-01

Background: Establishment of Early Warning Alert and Response System (EWARS) in Indonesia was initiated since 2009 in few selected provinces and government was planning to roll out in other provinces. Before initiating this roll out the assessment of performance of EWARS in 2012 was conducted. The aim of the assessment is to strengthen laboratory for supporting to EWARS for alerts testing. Methods: Laboratory capacity mapping tool and laboratory algorithm for twenty two priority diseases of ...

Aplikasi Npv At Risk dalam Analisis Kelayakan Finansial Budidaya Jamur Tiram Putih di Kabupaten Bogor Jawa Barat

OpenAIRE

Sarianti, Tintin; Sasongko, Hendro; Ratnawati, Anny

2008-01-01

The purpose of this study where to analyze white oyster mushroom farming activity in Sub-Province of Bogor, to analyze financial feasibility of white oyster mushroom farming activity for high and low land in Sub-Province of Bogor by NPV at Risk Method and to analyze the switching value for the changes of cost and benefit in white oyster mushroom farming activity. The data were collected from 6 farmer living in high and low land in Sub-Province of Bogor. The NPV at risk model and switching ...

Variasi Cakupan Jaminan Persalinan di Kabupaten Bogor 2011

Directory of Open Access Journals (Sweden)

Ajeng Tias Endarti

2012-12-01

health insurances coverage. Those who already had health insurance would not be eligible for Jampersal. It is recommended to increase the Jampersal promotion focused to the poor groups that have not been covered by any other health insurance. Keywords: evaluation, Jampersal, Distric of Bogor

APLIKASI NPV AT RISK DALAM ANALISIS KELAYAKAN FINANSIAL BUDIDAYA JAMUR TIRAM PUTIH DI KABUPATEN BOGOR JAWA BARAT

OpenAIRE

Tintin Sarianti; Hendro Sasongko; Anny Ratnawati

2011-01-01

The purpose of this study where to analyze white oyster mushroom farming activity in Sub-Province of Bogor, to analyze financial feasibility of  white oyster mushroom farming activity for high and low land in Sub-Province of Bogor by NPV at Risk Method and to analyze the switching value for the changes of cost and benefit in  white oyster mushroom farming activity.  The data were collected from 6 farmer living in high and low land in Sub-Province of Bogor. The NPV at risk model and switching ...

Cycad Aulacaspis Scale, a Newly Introduced Insect Pest in Indonesia

Directory of Open Access Journals (Sweden)

RANGASWAMY MUNIAPPAN

2012-09-01

Full Text Available Cycad aulacaspis scale (Aulacaspis yasumatsui Takagi (Hemiptera: Diaspididae is native to Thailand and Vietnam. Since the early 1990s it has been spreading around the world due to the trade in cycad plants for ornamental use. Infestation by this scale can kill cycads in only a few months. Its accidental introduction to Florida endangered the ornamental cycad-growing industry; and in Guam and Taiwan, endemic cycads (Cycas micronesica and C. taitungensis, respectively are currently threatened with extinction by cycad aulacaspis scale. In November 2011, an introduced scale was discovered damaging cycads in the Bogor Botanic Garden. Samples from Bogor were taken for identification of the scale, and the material was kept for some time to rear out any insect parasitoids that were present. Both the scale insects and parasitoids were prepared on microscope slides and studied microscopically for authoritative identification. The scale was confirmed as A. yasumatsui. The parasitoid Arrhenophagus chionaspidis Aurivillius (Hymenoptera: Encyrtidae and the hyperparasitoid Signiphora bifasciata Ashmead (Hymenoptera: Signiphoridae were identified from the samples. Unless immediate remedial measures are taken, several endemic species of cycad in Indonesia may be endangered by infestation by cycad aulacaspis scale.

PEMANFAATAN LIMBAH IKAN SIDAT INDONESIA (Anguilla bicolor SEBAGAI TEPUNG PADA INDUSTRI PENGOLAHAN IKAN DI PALABUHANRATU, KABUPATEN SUKABUMI

Directory of Open Access Journals (Sweden)

RA Hangesti Emi Widyasari

2014-05-01

Full Text Available ABSTRACTThis research aims to analyze the nutritive value of flour head, liver powder and bone meal as by product of Indonesian eel (Anguilla bicolor processing. Eel waste flour was made by using a thermal process in the drum dryer fish flouring mill PT. Carmelitha Lestari in Bogor, whereas proximate analysis for chemical tests were performed in the laboratory of Integrated Chemical Laboratory, IPB and direct observation was conducted in PT Jawa Suisan Indah, Palabuhanratu Sukabumi district in October 2012—April 2013. The nutritive value based on proximate analysis showed that head flour, liver flour, and bone flour contained protein 61.78%, 53.92%, and 41.01%; fat 15.55%, 27.28%, 13.07%; carbohydrate 11.48%; 14.96%, 8.13%; water 5.44%, 8.48%, 3.01%; ash 12.95%, 3.62%, 37.49%, and crude fiber 1.33%, 0.04%, 1.11%, respectively.Keywords: Anguilla bicolor, bone flour, head flour, liver flour, nutritive valueABSTRAKPenelitian ini bertujuan untuk menganalisis kandungan gizi tepung kepala, tepung tulang dan tepung hati ikan yang merupakan limbah pengolahan ikan sidat Indonesia (Anguilla bicolor. Tepung limbah ikan sidat dibuat berdasarkan proses termal menggunakan drum dryer di pabrik penepungan ikan PT. Carmelitha Lestari di Bogor dan analisis proksimat untuk uji kimiawi dilakukan di Laboratorium Kimia Terpadu, IPB serta observasi langsung di PT Jawa Suisan Indah, Palabuhanratu Kabupaten Sukabumi pada bulan Oktober 2012—April 2013. Hasil analisis proksimat tepung kepala, tepung hati dan tepung tulang mengandung protein berturut-turut sebesar 61.78%, 53.92%, dan 41.01%; lemak sebesar 15.55%; 27.28%; 13.07%, karbohidrat sebesar 11.48%; 14.96%; 8.13%, kadar air sebesar 5.44%; 8.48%; 3.01%, kadar abu 12.95%; 3.62%; 37.49% dan serat kasar 1.33%; 0.04%; 1.11%.Kata kunci: Anguilla bicolor, nilai gizi, tepung hati, tepung kepala, tepung tulang

ANALISIS PENGGUNAAN LAHAN DAN ARAHAN PENGENDALIAN PEMANFAATAN RUANG DI KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Ely Triwulan Dani

2017-02-01

Full Text Available Bogor Regency has three strategic functions, as a buffer zone of Jakarta, water system conservation area, and agricultural development area. Rapid population growth led to the need for land better place to live and businesses, it triggers a change in land use that resulted in the violation of spatial pattern plan. Therefore, the spatial usage must be controlled, in order to be in line with the spatial pattern plan and support functions of Bogor Regency. The main purpose of this study was to formulate the control direction of spatial usage in Bogor Regency. The spatial analysis used to obtain information on land use, land ownership, location permits, and evaluation of land use on spatial pattern plan. Schallogram method used to determine the level of the hierarchy of the region, and factors affecting the inconsistency of land use on spatial pattern plan obtained by the analysis of field data. Formulation of the directives controlling spatial utilization is obtained by descriptive analysis. The results showed that 51.33% of exsisting land use in Bogor Regency has not been done and still can be directed in order to conform with the spatial pattern plan, the remaining 38.06% was suitable and unsuitable 10.62%. The factors that cause such inconsistency were the lack socialization policy less, the absence of licensing, housing needs, public facilities availability and accessibility, lands of usage before defined spatial pattern plan, the transfer of land ownership, and sortage of agricultural support facilities. The implications of these results were necessary to apply four control instruments, those are licensing, zoning regulations, provision of incentives and disincentives, and sanctions. Besides, the need for policy dissemination and surveillance, monitoring and evaluation periodically.

Clinical bacteriology in low-resource settings: today's solutions.

Science.gov (United States)

Ombelet, Sien; Ronat, Jean-Baptiste; Walsh, Timothy; Yansouni, Cedric P; Cox, Janneke; Vlieghe, Erika; Martiny, Delphine; Semret, Makeda; Vandenberg, Olivier; Jacobs, Jan

2018-03-05

Low-resource settings are disproportionately burdened by infectious diseases and antimicrobial resistance. Good quality clinical bacteriology through a well functioning reference laboratory network is necessary for effective resistance control, but low-resource settings face infrastructural, technical, and behavioural challenges in the implementation of clinical bacteriology. In this Personal View, we explore what constitutes successful implementation of clinical bacteriology in low-resource settings and describe a framework for implementation that is suitable for general referral hospitals in low-income and middle-income countries with a moderate infrastructure. Most microbiological techniques and equipment are not developed for the specific needs of such settings. Pending the arrival of a new generation diagnostics for these settings, we suggest focus on improving, adapting, and implementing conventional, culture-based techniques. Priorities in low-resource settings include harmonised, quality assured, and tropicalised equipment, consumables, and techniques, and rationalised bacterial identification and testing for antimicrobial resistance. Diagnostics should be integrated into clinical care and patient management; clinically relevant specimens must be appropriately selected and prioritised. Open-access training materials and information management tools should be developed. Also important is the need for onsite validation and field adoption of diagnostics in low-resource settings, with considerable shortening of the time between development and implementation of diagnostics. We argue that the implementation of clinical bacteriology in low-resource settings improves patient management, provides valuable surveillance for local antibiotic treatment guidelines and national policies, and supports containment of antimicrobial resistance and the prevention and control of hospital-acquired infections. Copyright © 2018 Elsevier Ltd. All rights reserved.

KEGEMUKAN, ANEMIA, DAN PRESTASI BELAJAR SISWA SEKOLAH DASAR DI KOTA BOGOR

Directory of Open Access Journals (Sweden)

Lilis Heryati

2015-02-01

Full Text Available ABSTRACTThe purpose of this study was to analyze the correlation between overweight, anemia, and academic achievement of elementary school students in Bogor District. The design of this study was a cross-sectional and it was conducted between March to July 2014, at Polisi 1 and At Taufiq elementary school. Total subjects were 100 students, consisting of 62 students (62% with normal nutritional status and 38 students (38% overnutrition (overweight and obese. Mann Whitney test, Independent sample t test, Spearman and Pearson correlation test were applied to analyze the variables tested. The results showed that there were a significantly different intake of energy, protein, and fat (p<0.05, but not for anemic status and academic achievement among normal nutritional status and overnutrition of the subjects. A significant correlation was found between energy intake and nutritional status (p<0.05, anemic status and the mean scores of science and Indonesian language subjects (p<0.05. However, there were no significant correlation among nutritional status with anemic status and academic achievement (p>0.05.Keywords: academic achievement, anemia, obesity, overweightABSTRAKTujuan penelitian ini adalah menganalisis hubungan antara kegemukan, anemia, dan prestasi belajar siswa sekolah dasar di Kota Bogor. Desain penelitian yang digunakan adalah cross sectional study, dilaksanakan pada bulan Maret sampai Juli 2014, di Sekolah Dasar Negeri (SDN Polisi 1 dan Sekolah Dasar Islam Terpadu (SDIT At Taufiq. Jumlah subjek penelitian adalah 100 siswa, terdiri atas 62 siswa normal dan 38 siswa kegemukan (overweight dan obes. Analisis statistik yang digunakan adalah uji beda Mann Whitney dan independent sample t test serta uji korelasi Spearman dan Pearson. Hasil penelitian menunjukkan bahwa antara subjek normal dan kegemukan terdapat perbedaan yang signifikan pada asupan energi, protein, dan lemak (p<0,05, namun tidak terdapat perbedaan yang signifikan pada status

Environmental regulation of plant gene expression: an RT-qPCR laboratory project for an upper-level undergraduate biochemistry or molecular biology course.

Science.gov (United States)

Eickelberg, Garrett J; Fisher, Alison J

2013-01-01

We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands-on experience with RT-qPCR, the current "gold standard" for gene expression analysis, including detailed data analysis using the common 2-ΔΔCT method. Moreover, it provides a convenient starting point for many inquiry-driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature. Copyright © 2013 Wiley Periodicals, Inc.

[Inventive activity of the Departments of Chemistry and Biochemistry of Enzymes, and Protein Structure and Function of the Palladin Institute of Biochemistry of NAS of Ukraine. Part III. Diagnostic test-systems for analysis of fibrinolysis blood system and novel approaches to thrombosis treatment].

Science.gov (United States)

Danilova, V M; Vynogradova, R P; Chernysh, I Yu

2016-01-01

This article continues analysis of scientific achievements of the Institute of Biochemistry in the study of hemostasis system. Two previous articles were focused on the studies of blood coagulation proteins and development of the immune-enzyme test-systems for evaluation of the risk of thrombosis upon various pathologies. This article highlights the research on the blood fibrinolysis system and new approaches to thrombosis treatment, which were developed (and are under development) in the Palladin Institute of Biochemistry of the NAS of Ukraine, in particular, in the Department of Chemistry and Biochemistry of Enzymes headed previously by Dr.Sci.(Biol.) S. O. Kudinov and now by Dr.Sci.(Biol.) T .V. Grinenko, and also in the Department of Protein Structure and Function headed by Dr.Biol.Sci. E. M. Makogonenko. The fundamental knowledge of protein molecule functions and mechanisms of regulation of blood coagulation and fibrinolysis opens up new opportunities to diagnose hemostasis disorders and control the effectiveness of the cardiovascular disease treatment and also contributes to development of new techniques for isolation of new proteins – promising therapeutic agents.

National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines for use of tumor markers in clinical practice

DEFF Research Database (Denmark)

Sturgeon, Catharine M; Hoffman, Barry R; Chan, Daniel W

2008-01-01

BACKGROUND: This report presents updated National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines summarizing quality requirements for the use of tumor markers. METHODS: One subcommittee developed guidelines for analytical quality relevant to serum and tissue-based tumor...... questions to ensure selection of the appropriate test, adherence to good clinical and laboratory practices (e.g., minimization of the risk of incorrect patient and/or specimen identification, tube type, or timing), use of internationally standardized and well-characterized methods, careful adherence...... records. Also mandatory is extensive validation encompassing all stages of analysis before introduction of new technologies such as microarrays and mass spectrometry. Provision of high-quality tumor marker services is facilitated by dialogue involving researchers, diagnostic companies, clinical...

Medical Laboratory Technician--Microbiology (AFSC 90470).

Science.gov (United States)

Thompson, Joselyn H.

This four-volume student text is designed for use by Air Force personnel enrolled in a self-study extension course for medical laboratory technicians. Covered in the individual volumes are laboratory procedures in clinical bacteriology (the history of bacteriology; aseptic techniques and sterilization procedures; bacterial morphology and…

ANALISIS PERILAKU PELAJAR TERHADAP LINGKUNGAN STUDI KASUS SEKOLAH MENENGAH ATAS DI KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Barkatin Barkatin

2016-12-01

Full Text Available One of the causes of environmental problem in Indonesia is human factor, especially human behavior. Students as young generation play very important role on the awareness of environmental condition at school. There are several factors that influence the behavior intention towards environmental behavior of students, including the school environment, community environment, family environment, perception, attitude, subjective norm. Behavior intention affects how attitude of students on the environment. This study aimed to 1 analyze the influence of environmental of school, communities, families, perceptions, attitudes, subjective norm variable to the intention of behavior, 2 analyze the relationship between the intention of behavior to the behavior of students on the environment and 3 formulate strategies to be developed in the schools with regard to the environmental life. The results showed that the intention of behavior is influenced by subjective norms, perceptions, the school environment, attitude, family environment, community environment. SWOT analysis shows that Senior High School in Sub districts Parung,Ciseeng, Gunung Sindurof  Bogor Regency is in cell 5 (Growth/Stability.Strategy formulated by the study is introduction of environmental culture school or green school to schools and communities.

Doing that thing that scientists do: A discovery-driven module on protein purification and characterization for the undergraduate biochemistry laboratory classroom.

Science.gov (United States)

Garrett, Teresa A; Osmundson, Joseph; Isaacson, Marisa; Herrera, Jennifer

2015-01-01

In traditional introductory biochemistry laboratory classes students learn techniques for protein purification and analysis by following provided, established, step-by-step procedures. Students are exposed to a variety of biochemical techniques but are often not developing procedures or collecting new, original data. In this laboratory module, students develop research skills through work on an original research project and gain confidence in their ability to design and execute an experiment while faculty can enhance their scholarly pursuits through the acquisition of original data in the classroom laboratory. Students are prepared for a 6-8 week discovery-driven project on the purification of the Escherichia coli cytidylate kinase (CMP kinase) through in class problems and other laboratory exercises on bioinformatics and protein structure analysis. After a minimal amount of guidance on how to perform the CMP kinase in vitro enzyme assay, SDS-PAGE, and the basics of protein purification, students, working in groups of three to four, develop a protein purification protocol based on the scientific literature and investigate some aspect of CMP kinase that interests them. Through this process, students learn how to implement a new but perhaps previously worked out procedure to answer their research question. In addition, they learn the importance of keeping a clear and thorough laboratory notebook and how to interpret their data and use that data to inform the next set of experiments. Following this module, students had increased confidence in their ability to do basic biochemistry techniques and reported that the "self-directed" nature of this lab increased their engagement in the project. © 2015 The International Union of Biochemistry and Molecular Biology.

ANALYSIS OF SOCIAL MEDIA’S ACCESS; RELATION TO CHARACTER OF VOCATIONAL STUDENT IN BOGOR

Directory of Open Access Journals (Sweden)

Sudi Herlin Rahmawati

2014-05-01

Full Text Available Recently, the existence of social media in Indonesia is consequence of acculturation both of culture and technology. This study aimed to analyze the relation of social media on the teenagers’ character development, particularly responsibility, respectful, and courtesy. The observation was conducted in two vocational private high schools in Bogor, West Java Province. The selected schools were chosen as schools with high involvement on juvenile delinquency such as gang fight, drugs, and free-sex based on the data from local government official. Total participants were 100 students consist of 50 male and 50 female students at 1st and 2nd grade. Based on the analysis, there was significant difference between male and female student on accessing the social media (both on intensity and content. Females’ scored higher than male in accessing Facebook, Twitter, YouTube, etc. On average, females’ use of social media was between 2-3 hours per day, whereas for male it was only 1 hour. However, male had greater access to pornography than the females. The study found significant correlation between media social intensity and content access with the character. Highly teenagers’ access to social media; correlate with the low of responsibility, respectful and courtesy character. 

Keanekaragaman dan persebaran lalat buah Tribe Dacini (Diptera: Tephritidae di Kabupaten Bogor dan sekitarnya

Directory of Open Access Journals (Sweden)

Anik Larasati

2015-09-01

Full Text Available Bogor and its surrounding area is known as one of the region in West Java that has high diversity of horticultural plants that may have an affect on fruit fly diversity. Research on fruit fly diversity is not merely provide information on the species richness of fruit flys but also provide information on its distribution and dispersion. The aim of this research was to investigate the diversity and distribution of fruit flies species and their hosts in Bogor and its surrounding area. Fruit flies were collected from 119 sampling areas in Bogor Cianjur, Bekasi and Depok. Fruit flies were sampled using two methods, i.e. host rearing and trapping. Traps were modified from Lynfield traps and combined with two different attractants, i.e. metil eugenol (ME and Cue lure (CL. We found 18 species of fruit flies collected from traps and 24 host plants. The result showed that distribution, diversity and abundance of fruit flies was influenced by the diversity of host plants.

National survey on turnaround time of clinical biochemistry tests in 738 laboratories in China.

Science.gov (United States)

Zhang, Xiaoyan; Fei, Yang; Wang, Wei; Zhao, Haijian; Wang, Minqi; Chen, Bingquan; Zhou, Jie; Wang, Zhiguo

2018-02-01

This survey was initiated to estimate the current status of turnaround time (TAT) monitoring of clinical biochemistry in China, provide baseline data for establishment of quality specifications and analyze the impact factors of TAT. 738 laboratories were included. Questionnaires involved general information and data of related indicators of TAT during 1 week were provided to participating laboratories. Nine quality indicators were covered, which were medians, 90th and outlier rates of pre-examination, examination, and post-examination TAT. The 25th percentile, median, and 75th percentile of TATs were calculated as optimum, desirable, and minimum quality specifications. Percentages and sigma values were used to describe the outlier rates. Mann-Whitney and Kruskal-Wallis tests were used to identify the potential impacts of TAT. Response rate of this survey was 46.44%. More than 50% of the laboratories indicated they had set up target TATs in three time intervals and monitored TATs generally. The post-examination TAT of most laboratories was 0min, while the pre-examination and examination TAT varied. Sigma values of outlier rates for 45%~60% of laboratories were above 4, while 15%~20% of labs whose sigma values were below 3. Group comparisons suggested nurse or mechanical pipeline transportation, link laboratory information system with hospital information system, and using computer reporting instead of printing report were related to shorter TATs. Despite of the remarkable progresses of TATs in China, there was also room to improve. Laboratories should strengthen the construction of information systems, identify reasons for TAT delay to improve the service quality continuously. © 2017 Wiley Periodicals, Inc.

Keanekaragaman Dan Persebaran Lalat Buah Tribe Dacini (Diptera: Tephritidae) Di Kabupaten Bogor Dan Sekitarnya

OpenAIRE

Larasati, Anik; Hidayat, Purnama; Buchori, Damayanti

2013-01-01

Bogor and its surrounding area is known as one of the region in West Java that has high diversity of horticultural plants that may have an affect on fruit fly diversity. Research on fruit fly diversity is not merely provide information on the species richness of fruit flys but also provide information on its distribution and dispersion. The aim of this research was to investigate the diversity and distribution of fruit flies species and their hosts in Bogor and its surrounding area. Fruit fli...

Keanekaragaman dan persebaran lalat buah Tribe Dacini (Diptera: Tephritidae) di Kabupaten Bogor dan sekitarnya

OpenAIRE

Anik Larasati; Purnama Hidayat; Damayanti Buchori

2015-01-01

Bogor and its surrounding area is known as one of the region in West Java that has high diversity of horticultural plants that may have an affect on fruit fly diversity. Research on fruit fly diversity is not merely provide information on the species richness of fruit flys but also provide information on its distribution and dispersion. The aim of this research was to investigate the diversity and distribution of fruit flies species and their hosts in Bogor and its surrounding area. Fruit fli...

Croatian Society of Medical Biochemistry and Laboratory Medicine: national recommendations for venous blood sampling.

Science.gov (United States)

Nikolac, Nora; Supak-Smolcić, Vesna; Simundić, Ana-Maria; Celap, Ivana

2013-01-01

Phlebotomy is one of the most complex medical procedures in the diagnosis, management and treatment of patients in healthcare. Since laboratory test results are the basis for a large proportion (60-80%) of medical decisions, any error in the phlebotomy process could have serious consequences. In order to minimize the possibility of errors, phlebotomy procedures should be standardised, well-documented and written instructions should be available at every workstation. Croatia is one of the few European countries that have national guidelines for phlebotomy, besides the universally used CLSI (Clinical Laboratory Standards Institute) H3-A6 Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; approved Standard-Sixth Edition (CLSI, 2007) and WHO (World Health Organization) guidelines on drawing blood: best practices in phlebotomy (WHO, 2010). However, the growing body of evidence in importance of preanalytical phase management resulted in a need for evidence based revision and expansion of existing recommendations. The Croatian Society for Medical Biochemistry and Laboratory Medicine, Working Group for the Preanalytical Phase issued this recommendation. This document is based on the CLSI guideline H3-A6, with significant differences and additional information.

Diagnostic virology laboratory within a microbiology setting.

Science.gov (United States)

Rubin, S J

1984-01-01

The virology section at St. Francis Hospital and Medical Center, Connecticut, is not a separate laboratory division but is a part of the microbiology division and is supervised by the same personnel who supervise bacteriology, mycology, mycobacteriology, and serology. Current volume is over 1,000 cultures yearly with 12 to 24 percent positive. Isolates are confirmed and typed by the Connecticut State Health Department Laboratory. Specimen distribution, percentage positive specimens, and distribution of viral isolates are similar to those reported from microbiology laboratories with separate virology laboratories directed by a full-time doctoral-level virologist. Our seven years' experience demonstrates that a microbiology laboratory without a full-time doctoral-level virologist can provide clinically useful virologic information.

PENGARUH KONFLIK PERAN DAN STRES KERJA TERHADAP KOMITMEN ORGANISASI DI RUMAH SAKIT Tk IV SALAK, BOGOR

Directory of Open Access Journals (Sweden)

Sri Rulestri L.H.

2013-08-01

Full Text Available The study aims to determine whether there is effect role conflict and work stress to organization commitment at Rumah Sakit Tk IV Salak Bogor and also to get a valid and reliable data or fact, to prove it. The research has been done during April 2013. The method of this research is survey. The population research was all nurse of Rumah Sakit Tk IV Salak Bogor as much as 110 nurses. And the sample used as many 40 married women nurses by using simple random sampling. The result reveals that there is effect between role conflict to organization commitment, and also work stress to organiza-tion commitment, and then there is effect between role conflict and work stress to organization commitment of nurses Rumah Sakit Tk IV Salak, Bogor.

INVASIVE WEEDS IN BOGOR BOTANIC GARDENS, INDONESIA AND ITS IMPLICATION ON SURROUNDING LANDSCAPES

Directory of Open Access Journals (Sweden)

Edi Santosa

2014-07-01

Full Text Available Conservation areas with the objective for collection and exchange plant materials have been speculated as weed bank for surrounding areas. Objective of this study was to identify and characterize ruderal invasive weeds in the Bogor Botanic Gardens (BBG. Observations were conducted in all vak (collection blocks in the BBG in order to identify the weeds species, determine their invasiveness, dominance and distribution. Weeds associations with host plants were observed. Current weed control program and data of dead trees collection were analyzed in relevant to weed. Distribution of weeds outside BBG was observed by transects method following river and road directions. Results showed that there were seven invasive weeds, i.e., Cecropia adenopus (Cecropiaceae, Cissus nodosa Blume (Vitaceae, Cissus sicyoides Blume (Vitaceae, Dioscorea bulbifera L. (Dioscoreaceae, Ficus elastica Roxb. (Moraceae, Mikania micrantha H.B.K. (Asteraceae and Paraserianthes falcataria (L. Nielsen (Fabaceae. These seven weeds species invaded 41 out of 215 plant families in BBG. Six species of weeds, i.e., C. adenopus, C. nodosa Blume., C. sicyoides Blume., D. bulbifera L., M. micrantha H.B.K. and P. falcataria (L. Nielsen, were introduced as BBG collections for the first time while the F. elastica Roxb was considered as native. It is most likely that the weeds dispersal agents are the wind, birds, bats, visitors, and waters. All of these weeds existed in surrounding areas outside BBG. Given the detrimental impact of invasive weeds on the plant collection in BBG, it is necessary to develop long–term comprehensive control measures both inside and neighboring areas by involving other government authorities beyond BBG.

9 CFR 147.17 - Laboratory procedure recommended for the bacteriological examination of cull chicks and poults...

Science.gov (United States)

2010-01-01

... bacteriological examination of cull chicks from egg-type and meat-type chicken flocks and waterfowl, exhibition poultry, and game bird flocks and poults from turkey flocks for salmonella. (a) For cull chicks, from 25...

ISOLASI DAN IDENTIFIKASI BAKTERI PEREDUKSI SULFAT PADA AREA PERTAMBANGAN BATU BARA MUARA ENIM, SUMATERA SELATAN

Directory of Open Access Journals (Sweden)

Muchamad Yusron

2010-02-01

Full Text Available Sulfate reducing bacteria utilize sulfate as their terminal electron acceptor and reduce it to sulphide. Acid mine drainage, by-products of mining activities, is an acidic sulfate-rich wastewater suitable habitat for sulfate reducing bacteria. Isolation and identification of sulfate reducing bacteria collected from Muara Enim coal mining, South Sumatra was carried out at Laboratory of Environmental Biotechnology, Indonesian Center for Biodiversity and Biotechnology (ICBB, Bogor, and Laboratory of Microbiology, Faculty of Veterinary, Bogor Agricultural University. Postgate B liquid media was used for isolation and purification via serial dilution. Physiological and biochemical characterization was done based on Bergeys Manual of Determinative Bacteriology. Fifteen pure isolates have been isolated with diverse characteristics. Eight isolates can sustain at pH 3, while the rest sustain at pH 4 or above. Sulfate reduction efficiency of each isolates were different, but increased as the pH increased. The bacteria are classified as Desulfovibrio sp., which is characterized straight rods, motile, non spore-forming and able to grow in simple organic carbon.

Bacteriological water quality in the Lake Pontchartrain basin Louisiana following Hurricanes Katrina and Rita, September 2005

Science.gov (United States)

Stoeckel, Donald M.; Bushon, Rebecca N.; Demcheck, Dennis K.; Skrobialowski, Stanley C.; Kephart, Christopher M.; Bertke, Erin E.; Mailot, Brian E.; Mize, Scott V.; Fendick, Robert B.

2005-01-01

The U.S. Geological Survey (USGS), in collaboration with the Louisiana Department of Environmental Quality, monitored bacteriological quality of water at 22 sites in and around Lake Pontchartrain, La., for three consecutive weeks beginning September 13, 2005, following hurricanes Katrina and Rita and the associated flooding. Samples were collected and analyzed by USGS personnel from the USGS Louisiana Water Science Center and the USGS Ohio Water Microbiology Laboratory. Fecal-indicator bacteria (Escherichia coli, enterococci, and fecal coliform) concentrations ranged from the detection limit to 36,000 colony-forming units per 100 milliliters. Data are presented in tabular form and as plots of data in the context of available historical data and water-quality standards and criteria for each site sampled. Quality-control data were reviewed to ensure that methods performed as expected in a mobile laboratory setting.

Agreement of serum potassium measured by blood gas and biochemistry analyzer in patients with moderate to severe hyperkalemia.

Science.gov (United States)

Acikgoz, Seyyid Bilal; Genc, Ahmet Bilal; Sipahi, Savas; Yildirim, Mehmet; Cinemre, Behice; Tamer, Ali; Solak, Yalcin

2016-05-01

Several studies investigated the agreement between central laboratory biochemistry analyzers and blood gas analyzers for potassium measurements. However, data are scarce when the potassium level is moderate to severely high. We aimed to evaluate the agreement between central laboratory biochemistry analyzers and blood gas analyzer in terms of serum potassium level measurement because differences in potassium at this level translate into very different clinical actions. This was a retrospective medical record review study in which patients who presented to the emergency department and had serum potassium levels ≥6mmol/L were included. Patients who did not have simultaneous potassium measurement by blood gas analyzer were excluded. We included all patients meeting potassium criteria irrespective of their underlying disease or comorbidities. We evaluated agreement between the measurement methods with Pearson correlation, Bland-Altman plot, and Sign test. A total of 118 blood sample pairs were included. The mean serum potassium level measured by biochemistry analyzer was 6.78±0.79mmol/L, whereas it was 6.16±0.86mmol/L by blood gas analyzer (Pbiochemistry analyzer. The mean difference between the methods was 0.62±0.43mmol/L. In patients with moderate to severe hyperkalemia, blood gas analyzer and biochemistry analyzer gives significantly different serum potassium results which may be clinically important. Copyright © 2016 Elsevier Inc. All rights reserved.

Bringing the excitement and motivation of research to students; Using inquiry and research-based learning in a year-long biochemistry laboratory : Part II-research-based laboratory-a semester-long research approach using malate dehydrogenase as a research model.

Science.gov (United States)

Knutson, Kristopher; Smith, Jennifer; Nichols, Paul; Wallert, Mark A; Provost, Joseph J

2010-09-01

Research-based learning in a teaching environment is an effective way to help bring the excitement and experience of independent bench research to a large number of students. The program described here is the second of a two-semester biochemistry laboratory series. Here, students are empowered to design, execute and analyze their own experiments for the entire semester. This style of laboratory replaces a variety of shorter labs in favor of an in depth research-based learning experience. The concept is to allow students to function in independent research groups. The research projects are focused on a series of wild-type and mutant clones of malate dehydrogenase. A common research theme for the laboratory helps instructors administer the course and is key to delivering a research opportunity to a large number of students. The outcome of this research-based learning laboratory results in students who are much more confident and skilled in critical areas in biochemistry and molecular biology. Students with research experience have significantly higher confidence and motivation than those students without a previous research experience. We have also found that all students performed better in advanced courses and in the workplace. Copyright © 2010 International Union of Biochemistry and Molecular Biology, Inc.

HUBUNGAN KOMPETENSI DAN PELAKSANAAN WEWENANG BIDAN PRAKTIK MANDIRI DI KOTA BOGOR TAHUN 2013

Directory of Open Access Journals (Sweden)

Mieska Despitasari

2016-02-01

Full Text Available The reduction of maternal mortality rate is one of the MDGs targets that require efforts to achieve the target of 102 per 100,000 live births in 2015. The high maternal mortality rate in Indonesia can be caused by less adequate health care workforce, especially midwives. A midwive who provide care by herself, not as a part of a health care provider is called as a private midwives (BPM. Private midwives should have proper competencies while conducting her clinical practice. This analysis aimed to determine the effect of BPM competencies on the implementation of their authorities in Bogor city. This research involved 35 BPMs along with their superiors and colleagues for triangulation. The study was conducted by in-depth interviews and self administered questionnaires to assess the competencies and authorities of BPM. Samples were taken by snow ball sampling to find the eligible BPM which educated based on 2002 curricula. The relationship between competencies and authorities of BPM were analyzed by Pearson correlation test. The results showed that there was a significant relationship between the competencies of BPM and the implementation of their authorities (0.046 > α, with a Pearson correlation coefficient of 0.339. It showed that the higher the competencies of BPM , the higher the implemention of their authorities.

Preferensi Konsumen dan Produsen Produk Organik di Indonesia

Directory of Open Access Journals (Sweden)

Sri Muljaningsih

2013-09-01

Full Text Available Tujuan dari penelitian ini adalah untuk mempelajari kebiasaan konsumen dan produsen produk organik. Sampling penelitian ini kebetulan dilakukan pada pertemuan yang diselenggarakan oleh Aliansi Organik Indonesia (AOI. Pemantauan pasar dilakukan di Jakarta, Bogor, Depasar, dan Malang. Pengumpulan data dilakukan dengan wawancara langsung dengan responden. Responden terdiri atas 63 konsumen, 21 produsen produk organik. Analisis data dilakukan untuk mengetahui faktor apa yang mempengaruhi preferensi masing-masing individu. Hasil penelitian ini menunjukkan bahwa preferensi konsumen terhadap beras, buah, dan sayuran organik adalah baik untuk kesehatan, tetapi keluhannya produk tersebut lebih mahal. Preferensi produsen bahwa produk organik semisal beras, buah, dan sayuran adalah harus mempunyai sertifikat. Preferensi konsumen tidak dibatasi usia dan pendidikan, sedangkan preferensi produsen masih tidak memahami manfaat kesehatan dari produk organik.Kata kunci: konsumen,  produsen, produk organik, survey pasar

KEBIASAAN SARAPAN, STATUS GIZI, DAN KUALITAS HIDUP REMAJA SMP BOSOWA BINA INSANI BOGOR

Directory of Open Access Journals (Sweden)

Ilyatun Niswah

2014-11-01

Full Text Available ABSTRACTThe objective of this study was to examine the association between breakfast habits, nutritional status, and health related quality of life of adolescents in Bosowa Bina Insani Junior High School Bogor. A cross sectional study was conducted and simple random sampling was used to determine the subjects. Subjects were 60 adolescent students of Bosowa Bina Insani Junior High School Bogor aged 13—15 years old. The study found there was no significant difference in nutritional status between breakfast skippers and breakfast eaters (p>0.05. However, regular breakfast eaters were more likely to have lower body weight (r=-0.160, p=0.222. There was no significant difference of health related quality of life based on nutritional status (p>0.05. Breakfast eaters tended to have better quality of life than breakfast skippers even though there was no significant difference between them (p>0.05.Keywords: adolescents, breakfast habits, food consumption, health related quality of life, nutritionalstatusABSTRAKPenelitian ini bertujuan mengkaji hubungan kebiasaan sarapan, status gizi, dan kualitas hidup pada remaja SMP Bosowa Bina Insani Bogor. Desain penelitian ini adalah cross sectional dengan penarikan subjek secara simple random sampling. Subjek berjumlah 60 remaja berusia 13—15 tahun yang merupakan siswa-siswi SMP Bosowa Bina Insani Bogor. Hasil penelitian menunjukkan tidak terdapat perbedaan signifikan status gizi berdasarkan kebiasaan sarapan (p>0.05 namun terdapat kecenderungan semakin sering konsumsi sarapan, berat badan semakin menurun (r=-0.160, p=0.222. Tidak terdapat perbedaan signifikan kualitas hidup berdasarkan status gizi (p>0.05. Kualitas hidup pada kelompok yang biasa sarapan cenderung lebih tinggi dibandingkan kelompok yang tidak biasa sarapan, namun tidak terdapat perbedaan signifikan secara statistik (p>0.05.Kata kunci: kebiasaan sarapan, konsumsi pangan, kualitas hidup, remaja, status gizi

KERAGAAN PERTUMBUHAN BENIH IKAN MAS (Cyprinus carpio STRAIN MAJALAYA, LOKAL BOGOR DAN RAJADANU DI KOLAM CIJERUK, BOGOR-JAWA BARAT

Directory of Open Access Journals (Sweden)

Otong Zenal Arifin

2016-11-01

Full Text Available Penelitian ini bertujuan untuk mendapatkan strain ikan mas yang memiliki keragaan pertumbuhan yang baik sebagai spesies kandidat untuk program seleksi. Tiga strain ikan mas (majalaya, lokal Bogor, dan rajadanu dipelihara dalam jaring yang diletakkan di kolam Instalasi Penelitian Cijeruk, Bogor selama 6 minggu. Hasil penelitian menunjukkan bahwa tidak ada perbedaan nyata untuk parameter pertambahan bobot dan sintasan dari 3 strain ikan mas yang diuji, sedangkan untuk pertambahan panjang individual (mm per bulan ada perbedaan yang sangat nyata (P<0,05. Strain ikan mas rajadanu memiliki pertambahan panjang terbaik (16,0 ± 1,41 dan berbeda dengan majalaya (10,3 ± 4,50 serta lokal (10,8 ± 2,06. Objective of this study is to produce good performance of common carp by comparing three promising strains majalaya, local, and rajadanu. The study was carry out using floating net cages placed in earthen pond at The Cijeruk Germ Plasm Research Station. During the study, growth and survival rate were observed were observed for six weeks. The results indicated there was no significant difference for growth of weight and survival rate. However, growth of standard length was significantly different (P<0.05. Rajadanu made the best growth of standard length (16.0 ± 1.41 and was different from majalaya (10.3 ± 4.50 and local strain (10.8 ± 2.06.

The bacteriological screening of donated human milk: laboratory experience of British Paediatric Association's published guidelines.

Science.gov (United States)

Wright, K C; Feeney, A M

1998-01-01

This study was undertaken to assess the application of the British Paediatric Association's (BPA) published guidelines to the bacteriological screening of breast milk donated to a District General Hospital milk bank. Samples of donated milk were subjected to bacterial counts and provisional identification after both 24 and 48 h incubation on cysteine lactose electrolyte-deficient (CLED) and Columbia blood agar. 21.8% (76 out of 348) donations of milk failed to reach the BPA acceptable criteria. The organisms responsible for the rejection of these samples were all evident within 24 h incubation, and were not significantly confined to one medium. A large percentage of rejected samples originated from a small number of donor mothers; 63.2% came from one donor. In applying BPA guidelines, both CLED and Columbia blood agar were found to be equally effective in screening for unacceptable organisms in prepasteurization donated breast milk. The 24 h period allowed for bacteriological screening, prior to pasteurization of milk samples, was sufficient to allow the growth of all potentially pathogenic bacteria in this study. To prevent the donation of consistently contaminated milk, more active communication between the milk bank staff and the donor is recommended.

Urbanization and Land Use Changes in Peri-Urban Area using Spatial Analysis Methods (Case Study: Ciawi Urban Areas, Bogor Regency)

Science.gov (United States)

Cahya, D. L.; Martini, E.; Kasikoen, K. M.

2018-02-01

Urbanization is shown by the increasing percentage of the population in urban areas. In Indonesia, the percentage of urban population increased dramatically form 17.42% (1971) to 42.15% (2010). This resulted in increased demand for housing. Limited land in the city area push residents looking for an alternative location of his residence to the peri-urban areas. It is accompanied by a process of land conversion from green area into built-up area. Continuous land conversion in peri-urban area is becoming increasingly widespread. Bogor Regency as part of the Jakarta Metropolitan Area is experiencing rapid development. This regency has been experienced land-use change very rapidly from agricultural areas into urban built up areas. Aim of this research is to analyze the effect of urbanization on land use changes in peri-urban areas using spatial analysis methods. This research used case study of Ciawi Urban Area that experiencing rapid development. Method of this research is using descriptive quantitative approach. Data used in this research is primary data (field survey) and secondary data (maps). To analyze land use change is using Geographic Information System (GIS) as spatial analysis methods. The effect of urbanization on land use changes in Ciawi Urban Area from year 2013 to 2015 is significant. The reduction of farm land is around -4.00% and wetland is around - 2.51%. The increasing area for hotel/villa/resort is around 3.10%. Based on this research, local government (Bogor Regency) should be alert to the land use changes that does not comply with the land use plan and also consistently apply the spatial planning.

PENGARUH PRODUKTIVITAS TENAGA KERJA WANITA TERHADAP MUTU PRODUK MAKANAN INDUSTRI KECIL MENENGAH DI KOTA BOGOR

Directory of Open Access Journals (Sweden)

Siti Nur Fadlilah

2012-02-01

Full Text Available Industri makanan merupakan salah satu industri unggulan bagi sektor Industri Kecil Menengah (IKM di Kota Bogor. Berdasarkan pengamatan secara kualitatif yang dilakukan, banyak tenaga kerja wanita yang bekerja dibidang industri makanan. Perumusan masalah dalam penelitian ini adalah seberapa tinggi pengaruh produktifitas tenaga kerja wanita terhadap mutu produk makanan IKM.  Penelitian ini bertujuan untuk mengetahui hubungan antara produktifitas kerja wanita dengan mutu produk yang dihasilkan. Pengolahan data dilakukan dengan mereduksi jumlah industri IKM potensial yang akan dijadikan obyek penelitian dengan kriteria tertentu, dengan menggunakan pendekatan Analytical Hierarchy Process (AHP. Pengukuran produktifitas dilakukan berdasarkan pendekatan Rasio Output/Input. Penentuan hubungan pengaruh produktifitas tenaga kerja wanita dengan mutu produk makanan IKM adalah berdasarkan pendekatan koefisien korelasi. Hasil penelitian ini menyatakan bahwa IKM produk makanan yang berpotensi di Kota Bogor adalah IKM produsen nata de coco, dimana dengan pendekatan AHP diperoleh bobot tertinggi dibandingkan dengan 28 IKM produk makanan lainnya, yaitu sebesar 0,096. Berdasarkan 4 IKM produsen nata de coco, dapat diambil kesimpulan secara umum, bahwa pada IKM nata de coco di Kota Bogor tidak terdapat pengaruh antara produktivitas tenaga kerja wanita dengan mutu produk. Kata kunci: IKM, Produktifitas, AHP, Analisa Korelasi     Food Industri is one of a potensial small medium interprise in Bogor. Based on qualitative observation, more women working at food industries. The problem in this research is How much the relationship between women worker with SME’s food product quality. The goal of this research is to find out the correlation between women worker’s productivity with product quality. This problem will be solve by reduce many potensial SME with some criteria use AHP. Productivity meseaure with Output/Input ratio. Correlation analysis used to know about

Raman Investigation of Temperature Profiles of Phospholipid Dispersions in the Biochemistry Laboratory

Science.gov (United States)

Craig, Norman C.

2015-06-01

The temperature dependence of self-assembled, cell-like dispersions of phospholipids is investigated with Raman spectroscopy in the biochemistry laboratory. Vibrational modes in the hydrocarbon interiors of phospholipid bilayers are strongly Raman active, whereas the vibrations of the polar head groups and the water matrix have little Raman activity. From Raman spectra increases in fluidity of the hydrocarbon chains can be monitored with intensity changes as a function of temperature in the CH-stretching region. The experiment uses detection of scattered 1064-nm laser light (Nicolet NXR module) by a Fourier transform infrared spectrometer (Nicolet 6700). A thermoelectric heater-cooler device (Melcor) gives convenient temperature control from 5 to 95°C for samples in melting point capillaries. Use of deuterium oxide instead of water as the matrix avoids some absorption of the exciting laser light and interference with intensity observations in the CH-stretching region. Phospholipids studied range from dimyristoylphosphotidyl choline (C14, transition T = 24°C) to dibehenoylphosphotidyl choline (C22, transition T = 74°C).

Bacteriological And Clinical Evaluation Of Twelve Cases Of Post ...

African Journals Online (AJOL)

Bacteriological And Clinical Evaluation Of Twelve Cases Of Post-Surgical Sepsis Of Odontogenic Tumours At A ... East African Medical Journal ... Intervention: Adequate review of patient\\'s medical history, bacteriological investigations and

Rapid Clinical Bacteriology and Its Future Impact

Science.gov (United States)

Durand, Géraldine; Peyret, Michel; Chatellier, Sonia; Zambardi, Gilles; Schrenzel, Jacques; Shortridge, Dee; Engelhardt, Anette; Dunne, William Michael

2013-01-01

Clinical microbiology has always been a slowly evolving and conservative science. The sub-field of bacteriology has been and still is dominated for over a century by culture-based technologies. The integration of serological and molecular methodologies during the seventies and eighties of the previous century took place relatively slowly and in a cumbersome fashion. When nucleic acid amplification technologies became available in the early nineties, the predicted "revolution" was again slow but in the end a real paradigm shift did take place. Several of the culture-based technologies were successfully replaced by tests aimed at nucleic acid detection. More recently a second revolution occurred. Mass spectrometry was introduced and broadly accepted as a new diagnostic gold standard for microbial species identification. Apparently, the diagnostic landscape is changing, albeit slowly, and the combination of newly identified infectious etiologies and the availability of innovative technologies has now opened new avenues for modernizing clinical microbiology. However, the improvement of microbial antibiotic susceptibility testing is still lagging behind. In this review we aim to sketch the most recent developments in laboratory-based clinical bacteriology and to provide an overview of emerging novel diagnostic approaches. PMID:23301218

PENERAPAN MODEL BRAND EQUITY TEN PADA UPBJJ-UT BOGOR

Directory of Open Access Journals (Sweden)

Deni Surapto

2015-04-01

Full Text Available The objectives of the study are to (1 analyze the dimensions of awareness, associations, perceived quality/leadership, loyalty, and market behavior based upon the assessment of Open University students and the public, (2 evaluate the Open University brand equity model based on brand equity ten. The time period of research was during April-July 2012 and the location was in Open University Bogor campus. The population of this study consisted of high school students, employees and students of Open University Bogor, amounting to 693,950 people. Samples of 400 people were obtained. The data is collected using survey method, whereby interviews of the sample group is conducted using a closed form questionnaire based upon the Likert scale method. Based on the results from the analysis of the measures of awareness and brand recall, the Open University got the ranking of 11th and 7th among the other universities from 2% of the respondents respectively. It was ranked 1st on the measure of recognition and unaware by 5.5% of respondents. Based on the results from the analysis of the measure of association using Cochran test, it was found that the attributes of time and cost are associated with the brand of Open University. Results from the analysis of the measure of perceived quality regarding quality of services provided by Open University to its students show that registration services rated good while study material, online tutorials and exams were assessed as fair. Result from the analysis of the price premium loyalty measures indicate that students objected to an increase in tuition over Rp.25.000,-. Satisfaction measure indicates that the level of satisfaction of the Open University students is up to the extent of liking the brand, that is like and enjoy the study at Open University. This is indicated by the highest score received for the level of liking the brand which is 70%. Result from the analysis of the measure market behavior regarding market

Current practice in laboratory diagnostics of autoimmune diseases in Croatia. 
Survey of the Working group for laboratory diagnostics of autoimmune diseases of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Science.gov (United States)

Kuna, Andrea Tešija; Đerek, Lovorka; Kozmar, Ana; Drvar, Vedrana

2016-10-15

With the trend of increasing incidence of autoimmune diseases, laboratories are faced with exponential growth of the requests for tests relating the diagnosis of these diseases. Unfortunately, the lack of laboratory personnel experienced in this specific discipline of laboratory diagnostic, as well as an unawareness of a method limitation often results in confusion for clinicians. The aim was to gain insight into number and type of Croatian laboratories that perform humoral diagnostics with the final goal to improve and harmonize laboratory diagnostics of autoimmune diseases in Croatia. In order to get insight into current laboratory practice two questionnaires, consisting of 42 questions in total, were created. Surveys were conducted using SurveyMonkey application and were sent to 88 medical biochemistry laboratories in Croatia for the first survey. Out of 33 laboratories that declared to perform diagnostic from the scope, 19 were selected for the second survey based on the tests they pleaded to perform. The survey comprised questions regarding autoantibody hallmarks of systemic autoimmune diseases while regarding organ-specific autoimmune diseases was limited to diseases of liver, gastrointestinal and nervous system. Response rate was high with 80 / 88 (91%) laboratories which answered the first questionnaire, and 19 / 19 (1.0) for the second questionnaire. Obtained results of surveys indicate high heterogeneity in the performance of autoantibody testing among laboratories in Croatia. Results indicate the need of creating recommendations and algorithms in order to harmonize the approach to laboratory diagnostics of autoimmune diseases in Croatia.

Symposium 19: Federal University of Rio Grande do Sul: its Role in the Biochemistry Teaching in the Southernmost Brazilian States

Directory of Open Access Journals (Sweden)

Clóvis M.D. Wannmacher

2014-08-01

Full Text Available K-Education(Portuguese Chair: V. Trindade Bayardo Torres; Clovis Wannmacher; Denise Macedo  Federal University of Rio Grande do Sul: its Role in the Biochemistry Teaching in the Southernmost Brazilian States. Wannmacher, C.M.D. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.   At present, most Biochemistry teaching in Rio Grande do Sul and Santa Catarina has its origin in the Department of Biochemistry of the Federal University of Rio Grande do Sul. In the 70’s, all the teachers of the Department wrote a book together: “Fundamental Biochemistry”. This book was always used in the classes by groups of students of different careers supervised by the respective teacher. At the same time, this methodology was not used by pharmaceutical teachers, but they recommended the Department’s book to their students. Along the next years, Biochemistry teaching was adapted according to the professional course and to the teacher’s personal characteristics. Today, there are two extremes strategies again: one traditional for the most basic biochemistry student’s formation (including theoretical, laboratorial, seminars and informatics classes and the other, experimental-clinical, for physician’s formation (including seminars of molecular approach to the most prevalent diseases, mainly, those leading to failure of organs/systems and the interaction with patients from HCPA by the interpretation of their biochemical data. On the other hand, the Post Graduation Program, at first, emphasized biochemistry teaching in a traditional form, but gradually changed the emphasis to investigation, and most classes changed to scientific paper reports. To stimulate the teaching formation, two activities were offered to post-graduation students: Biochemistry Teaching Methodology and Teaching Practice in Biochemistry. These activities promote opportunity for the students to

Experiences from introduction of peer-to-peer teaching methods in Advanced Biochemistry E2010

DEFF Research Database (Denmark)

Brodersen, Ditlev; Etzerodt, Michael; Rasmussen, Jan Trige

2012-01-01

During the autumn semester 2010, we experimented with a range of active teaching methods on the course, Advanced Biochemistry, at the Department of Molecular Biology and Genetics.......During the autumn semester 2010, we experimented with a range of active teaching methods on the course, Advanced Biochemistry, at the Department of Molecular Biology and Genetics....

BOREAS TE-9 NSA Canopy Biochemistry

Science.gov (United States)

Hall, Forrest G. (Editor); Curd, Shelaine (Editor); Margolis, Hank; Charest, Martin; Sy, Mikailou

2000-01-01

The BOREAS TE-9 team collected several data sets related to chemical and photosynthetic properties of leaves. This data set contains canopy biochemistry data collected in 1994 in the NSA at the YJP, OJR, OBS, UBS, and OA sites, including biochemistry lignin, nitrogen, cellulose, starch, and fiber concentrations. These data were collected to study the spatial and temporal changes in the canopy biochemistry of boreal forest cover types and how a high-resolution radiative transfer model in the mid-infrared could be applied in an effort to obtain better estimates of canopy biochemical properties using remote sensing. The data are available in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

[Inventive activity of the Department of Molecular Immunology of the Palladin Institute of Biochemistry of NAS of Ukraine].

Science.gov (United States)

Danilova, V M; Vynogradova, R P; Torkhova, S G

2016-01-01

The article is devoted to the inventive activity of the Department of Molecular Immunology of the Palladin Institute of Biochemistry of NAS of Ukraine in the context of the history of its inception, development and in the context of scholarly and organizational activities of Sergii Vasyl’ovych Komisarenko. This autumn marks 50th anniversary since young Sergii Komisarenko (now – Academician of NAS and NAMS of Ukraine, Dr. Biol. Sci., Professor) has joined the Palladin Institute of Biochemistry, has completed all stages of the academic carrier from PhD student to Head of the Institute. He is the first in Ukraine who started the new branch of research – molecular immunology, created a strong scientific school, which earned worldwide acclaim and made significant contribution to finding solutions to current problems in human health sciences. S.V. Komisarenko was among those, who were first in the USSR to use immunoenzyme and flow cytofluometric assays, hybridoma technology for producing monoclonal antibodies and immunochemical assay of proteins, which became the basis for development of highly sensitive and highly specific immunodiagnostic systems, which are of high necessity in medicine, veterinary, development of immunotechnologies, environment monitoring, etc. Under his leadership the Department has made a series of important discoveries and developments including relating to antitumour immunotoxins, effects of low dose radiation on the immune system of Chernobyl liquidators, immunochemical structure of neurotoxin apamine, cytochrom c, fibrinogen and fibrin molecules at different stages of polymerization, diphtheria toxin and its receptor, tuberculosis causing micobacterium, roles of protease-activated receptors (PARs) and nicotinic acetylcholine receptors of lymphocytes, nature of polyreactive immunoglobulins (PRIGs), among other important scientific contributions. S.V. Komisarenko and his colleagues also hold numerous (more than 80) author’s certificates

Computerized Laboratories in an Undergraduate Psychology Department.

Science.gov (United States)

Brazier, Mary M.

A computer project sponsored by a National Science Foundation grant was completed in the psychology department at Loyola University. The purpose of the project was to upgrade existing laboratory equipment in both the operant learning and sensation/perception laboratories, to provide equipment for a cognition laboratory, and to allow increased and…

Evaluation of Pulasan (Nephelium ramboutan-ake Genetic Diversity in Bogor, West Java, Using Microsatellite Markers

Directory of Open Access Journals (Sweden)

Amelia Luisyane Puhili

2016-11-01

Full Text Available Pulasan (Nephelium ramboutan-ake (Labill. Leenh fruit is highly similar to rambutan rapiah (Nephelium lappaceum fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA9(CA2(GA4 dan LMLY12 (CT11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na, the number of effective alleles (Ne, Shannon information index (I, heterozygosity (He, and the percentage of polymorphism (PLP. The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313. The genetic diversity within a population (61% was higher than that among populations (39%. A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA. The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.

The quality and scope of information provided by medical laboratories to patients before laboratory testing: Survey of the Working Group for Patient Preparation of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Science.gov (United States)

Nikolac, Nora; Simundic, Ana-Maria; Kackov, Sanja; Serdar, Tihana; Dorotic, Adrijana; Fumic, Ksenija; Gudasic-Vrdoljak, Jelena; Klenkar, Kornelija; Sambunjak, Jadranka; Vidranski, Valentina

2015-10-23

The aim of this work was to evaluate to what extent the scope and content of information provided to patients is standardized across medical biochemistry laboratories in Croatia. Two on-line self-report surveys were sent out: Survey A regarding attitudes on importance of patient preparation and Survey B on the contents of patient preparation instructions. 13/118 laboratories (11%) do not provide written instructions to patients on how to prepare for laboratory testing, and 36 (40%) do not include information about water intake in their instructions. Only half of laboratories provide instructions for prostate-specific antigen (53.8%), female sex hormones (53.7%) and therapeutic drug monitoring (TDM) (52.5%). Inadequate information about fasting status (55.0%) and 24 hour urine collection (77.9%) were frequent errors with high severity and were associated with the greatest potential to cause patient harm. Laboratory professionals in Croatia have a positive attitude towards the importance of patient preparation for laboratory testing. However, the information for laboratory testing is not standardized and frequently lacks guidance for tests related to TDM, coagulation and endocrinology. This study highlights the need for standardized, updated and evidence-based recommendations for patient preparation in order to minimize the risk for patients. Copyright © 2015 Elsevier B.V. All rights reserved.

[Inventive activity of the Department of Metabolism Regulation of the Palladin Institute of Biochemistry of NAS of Ukraine].

Science.gov (United States)

Danilova, V M; Vynogradova, R P; Chernysh, I G; Petrenko, T M

2016-01-01

The article is devoted to the inventive activity of the Department of Metabolism Regulation of the Palladin Institute of Biochemistry of NAS of Ukraine in the context of the history of its inception, development and the research activities of its founder, academician of NAS of Ukraine M. F. Guly as well as his students and followers. It briefly tells about practical achievements of M. F. Guly which were as significant, immense and diverse as his scientific accomplishments. The paper analyses in detail the practical results of scientific research of his students and followers aimed to solve practical problems of medicine, food-processing, agriculture, and which are essentially a continuation of the ideas and projects of M. F. Guly.

Infestasi Pinjal dan Infeksi Dipylidium Caninum Linnaeus pada Kucing Liar di Lingkungan Kampus Institut Pertanian Bogor, Kecamatan Dramaga

OpenAIRE

Bashofi, Aulia Syifak; Soviana, Susi; Ridwan, Yusuf

2015-01-01

This study was conducted to investigate flea infestation and Dipylidium caninum (Linnaeus 1758) infection on stray cat on Bogor Agricultural University Dramaga. Thirty stray cats were collected from various places around on Bogor Agricultural University Dramaga. The stray cats that showed clinical signs of pruritus and alopecia were collected purposively. The whole body was powdered by gamexan powder, after that the fleas were collected by manual and examined microscopically. The totals of 30...

Titration of Alanine Monitored by NMR Spectroscopy: A Biochemistry Laboratory Experiment

Science.gov (United States)

Waller, Francis J.; And Others

1977-01-01

The experiment described here involves simultaneous monitoring of pH and NMR chemical shifts during an aqueous titration of alpha- and beta-alanine. This experiment is designed for use in an undergraduate biochemistry course. (MR)

75 FR 8147 - Notice of Consideration of Amendment Request for Decommissioning of Analytical Bio-Chemistry...

Science.gov (United States)

2010-02-23

... NUCLEAR REGULATORY COMMISSION [Docket No. 030-05154; NRC-2010-0056] Notice of Consideration of Amendment Request for Decommissioning of Analytical Bio-Chemistry Laboratories, Inc. Sanitary Lagoon... license amendment to Byproduct Material License No. 24- 13365-01 issued to Analytical Bio-Chemistry...

Capillary blood sampling: national recommendations on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Science.gov (United States)

Krleza, Jasna Lenicek; Dorotic, Adrijana; Grzunov, Ana; Maradin, Miljenka

2015-01-01

Capillary blood sampling is a medical procedure aimed at assisting in patient diagnosis, management and treatment, and is increasingly used worldwide, in part because of the increasing availability of point-of-care testing. It is also frequently used to obtain small blood volumes for laboratory testing because it minimizes pain. The capillary blood sampling procedure can influence the quality of the sample as well as the accuracy of test results, highlighting the need for immediate, widespread standardization. A recent nationwide survey of policies and practices related to capillary blood sampling in medical laboratories in Croatia has shown that capillary sampling procedures are not standardized and that only a small proportion of Croatian laboratories comply with guidelines from the Clinical Laboratory Standards Institute (CLSI) or the World Health Organization (WHO). The aim of this document is to provide recommendations for capillary blood sampling. This document has been produced by the Working Group for Capillary Blood Sampling within the Croatian Society of Medical Biochemistry and Laboratory Medicine. Our recommendations are based on existing available standards and recommendations (WHO Best Practices in Phlebotomy, CLSI GP42-A6 and CLSI C46-A2), which have been modified based on local logistical, cultural, legal and regulatory requirements. We hope that these recommendations will be a useful contribution to the standardization of capillary blood sampling in Croatia.

EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry and Laboratory Medicine: version 3 - 2005.

Science.gov (United States)

Zerah, Simone; McMurray, Janet; Bousquet, Bernard; Baum, Hannsjorg; Beastall, Graham H; Blaton, Vic; Cals, Marie-Josèphe; Duchassaing, Danielle; Gaudeau-Toussaint, Marie-Françoise; Harmoinen, Aimo; Hoffmann, Hans; Jansen, Rob T; Kenny, Desmond; Kohse, Klaus P; Köller, Ursula; Gobert, Jean-Gérard; Linget, Christine; Lund, Erik; Nubile, Giuseppe; Opp, Matthias; Pazzagli, Mario; Pinon, Georges; Queralto, José M; Reguengo, Henrique; Rizos, Demetrios; Szekeres, Thomas; Vidaud, Michel; Wallinder, Hans

2006-01-01

The EC4 Syllabus for Postgraduate Training is the basis for the European Register of Specialists in Clinical Chemistry and Laboratory Medicine. The syllabus: Indicates the level of requirements in postgraduate training to harmonise the postgraduate education in the European Union (EU); Indicates the level of content of national training programmes to obtain adequate knowledge and experience; Is approved by all EU societies for clinical chemistry and laboratory medicine. The syllabus is not primarily meant to be a training guide, but on the basis of the overview given (common minimal programme), national societies should formulate programmes that indicate where knowledge and experience is needed. The main points of this programme are: Indicates the level of requirements in postgraduate training to harmonise the postgraduate education in the European Union (EU); Indicates the level of content of national training programmes to obtain adequate knowledge and experience; Is approved by all EU societies for clinical chemistry and laboratory medicine. Knowledge in biochemistry, haematology, immunology, etc.; Pre-analytical conditions; Evaluation of results; Interpretations (post-analytical phase); Laboratory management; and Quality insurance management. The aim of this version of the syllabus is to be in accordance with the Directive of Professional Qualifications published on 30 September 2005. To prepare the common platforms planned in this directive, the disciplines are divided into four categories: Indicates the level of requirements in postgraduate training to harmonise the postgraduate education in the European Union (EU); Indicates the level of content of national training programmes to obtain adequate knowledge and experience; Is approved by all EU societies for clinical chemistry and laboratory medicine. Knowledge in biochemistry, haematology, immunology, etc.; Pre-analytical conditions; Evaluation of results; Interpretations (post-analytical phase); Laboratory

From gene to structure: Lactobacillus bulgaricus D-lactate dehydrogenase from yogurt as an integrated curriculum model for undergraduate molecular biology and biochemistry laboratory courses.

Science.gov (United States)

Lawton, Jeffrey A; Prescott, Noelle A; Lawton, Ping X

2018-05-01

We have developed an integrated, project-oriented curriculum for undergraduate molecular biology and biochemistry laboratory courses spanning two semesters that is organized around the ldhA gene from the yogurt-fermenting bacterium Lactobacillus bulgaricus, which encodes the enzyme d-lactate dehydrogenase. The molecular biology module, which consists of nine experiments carried out over eleven sessions, begins with the isolation of genomic DNA from L. bulgaricus in yogurt and guides students through the process of cloning the ldhA gene into a prokaryotic expression vector, followed by mRNA isolation and characterization of recombinant gene expression levels using RT-PCR. The biochemistry module, which consists of nine experiments carried out over eight sessions, begins with overexpression of the cloned ldhA gene and guides students through the process of affinity purification, biochemical characterization of the purified LdhA protein, and analysis of enzyme kinetics using various substrates and an inhibitor, concluding with a guided inquiry investigation of structure-function relationships in the three-dimensional structure of LdhA using molecular visualization software. Students conclude by writing a paper describing their work on the project, formatted as a manuscript to be submitted for publication in a scientific journal. Overall, this curriculum, with its emphasis on experiential learning, provides hands-on training with a variety of common laboratory techniques in molecular biology and biochemistry and builds experience with the process of scientific reasoning, along with reinforcement of essential transferrable skills such as critical thinking, information literacy, and written communication, all within the framework of an extended project having the look and feel of a research experience. © 2018 by The International Union of Biochemistry and Molecular Biology, 46(3):270-278, 2018. © 2018 The International Union of Biochemistry and Molecular Biology.

Bacteriological monitoring of unheated human milk.

Science.gov (United States)

Davidson, D C; Poll, R A; Roberts, C

1979-10-01

To assess the bacteriological quality of unpooled expressed breast milk, a pilot bottle sample of each donation was examined before the milk was given to the neonate. Provided the milk did not contain greater than 2500 organisms/ml or potential pathogens it was used unheated. Milk containing between 2500 and 5000 organisms/ml and no potential pathogens was used after pasteurisation. Using these criteria, 67% of 460 donations were acceptable. However, because the bacteriological quality varied, 45% of domiciliary donations were discarded compared with only 29% of those from hospital.

Hematology, plasma chemistry, and bacteriology of wild Tundra Swans (Cygnus columbianus) in Alaska.

Science.gov (United States)

Milani, Juliana F; Wilson, Heather; Ziccardi, Michael; LeFebvre, Rance; Scott, Cheryl

2012-01-01

Blood and cloacal swabs were collected from 100 (66 female, 34 male) wild Tundra Swans (Cygnus columbianus) molting in northwestern Alaska, USA, 25-28 July 2008, to establish hematologic and serum chemistry reference values and to isolate enteric Salmonella spp. and Escherichia coli O157:H7. Plasma biochemistry and hematology values did not vary significantly by sex or age. Tundra swans had high levels of creatine kinase, lactate dehydrogenase, amylase, and alkaline phosphatase compared with some other avian species (values were up to 7 times greater), possibly indicating capture myopathy. However, concentrations were much lower (up to 8 times lower) than in other waterfowl exposed to similar or more intensive capture methods. White blood cell count and hematocrit values were similar to other waterfowl species, and enteric Salmonella spp. and E. coli O157:H7 were not present among birds sampled. Our data provide the first biochemical, hematologic, and bacteriologic reference values for wild Tundra Swans.

Developing and Supporting Students' Autonomy to Plan, Perform, and Interpret Inquiry-Based Biochemistry Experiments

Science.gov (United States)

Silva, Thanuci; Galembeck, Eduardo

2017-01-01

Laboratory sessions are designed to develop the experimental skills and the acquaintance with instruments that may contribute to a successful career in Biochemistry and associated fields. This study is a report on improving a traditional Biochemistry course by devising the laboratory sessions as an inquiry-based environment to develop the…

Unusual clustering of coefficients of variation in published articles from a medical biochemistry department in India.

Science.gov (United States)

Hudes, Mark L; McCann, Joyce C; Ames, Bruce N

2009-03-01

A simple statistical method is described to test whether data are consistent with minimum statistical variability expected in a biological experiment. The method is applied to data presented in data tables in a subset of 84 articles among more than 200 published by 3 investigators in a small medical biochemistry department at a major university in India and to 29 "control" articles selected by key word PubMed searches. Major conclusions include: 1) unusual clustering of coefficients of variation (CVs) was observed for data from the majority of articles analyzed that were published by the 3 investigators from 2000-2007; unusual clustering was not observed for data from any of their articles examined that were published between 1992 and 1999; and 2) among a group of 29 control articles retrieved by PubMed key word, title, or title/abstract searches, unusually clustered CVs were observed in 3 articles. Two of these articles were coauthored by 1 of the 3 investigators, and 1 was from the same university but a different department. We are unable to offer a statistical or biological explanation for the unusual clustering observed.

Lignin biochemistry and soil N determine crop residue decomposition and soil priming

Science.gov (United States)

Cropping history can affect soil properties, including available N, but little is known about the interactive effects of residue biochemistry, temperature and cropping history on residue decomposition. A laboratory incubation examined the role of residue biochemistry and temperature on the decomposi...

Inventive activity of the Department of Metabolism Regulation of the Palladin Institute of Biochemistry of NAS of Ukraine

Directory of Open Access Journals (Sweden)

V. M. Danilova

2016-12-01

Full Text Available The article is devoted to the inventive activity of the Department of Metabolism Regulation of the Palladin Institute of Biochemistry of NAS of Ukraine in the context of the history of its inception, development and the research activities of its founder, academician of NAS of Ukraine M. F. Guly as well as his students and followers. It briefly tells about practical achievements of M. F. Guly which were as significant, immense and diverse as his scientific accomplishments. The paper analyses in detail the practical results of scientific research of his students and followers aimed to solve practical problems of medicine, food-processing, agriculture, and which are essentially a continuation of the ideas and projects of M. F. Guly.

Management of Hazardous Waste in Indonesia

Science.gov (United States)

Widyatmoko, H.

2018-01-01

Indonesia needs to build four Treatment Centrals for 229,907 tons per year produced hazardous waste. But almost all hazardous waste treatment is managed by just one company at present, namely PT. PPLI (Prasada Pamunah Limbah Industri). This research is based on collected data which identifies payback period of 0.69 years and rate of return 85 %. PT PPLI is located within the Cileungsi District of the Bogor Regency of West Java Province. Records from nearest rainfall station at Cibinong indicate that annual average rainfall for the site is about 3,600 mm. It is situated on hilly terrain and is characterized by steep slopes as well as has a very complex geological structure. The Tertiary sequence was folded to form an assymetric anticline with axis trend in an East-West direction. Three major faults cut the middle of the site in a North-South direction with a vertical displacement of about 1.5 meters and a zone width of 1 meter. The high concentration of Chemical Oxygen Demand (COD) 2500 ppm in Secondary Leachate Collection System (SLCS) indicate a possible failure of the Primary Leachate Clection System (PLCS), which need correct action to prevent groundwater contamination.

Rantai Nilai Pada Budidaya Kolam Ikan Air Tawar di Kota Bogor

Directory of Open Access Journals (Sweden)

Arief Nur Rakhman

2017-09-01

Full Text Available This study was conducted to determine the flow of the value chain of freshwater fish pond culture in the city. Research on the value chain actors freshwater fish farming in Bogor, mapping the value chain, the production cost and the Marginal value of the value chain. Actors involved in the value chain of freshwater fish farming pond in the city of Bogor is fish farmers, collectors and traders. From the analysis of the Margin, the mediator has the greatest Margin value.Keywords: fish culture, value chain, freshwater fish, value-chain analysis (VCAAbstrak: Penelitian ini dilakukan untuk mengetahui alur rantai nilai budidaya kolam ikan air tawar di Kota. Anlisis penelitian yang digunakan adalah analisis rantai nilai/value-chain analysis (VCA. Hasil penelitian menunjukkan secara umum pada rantai nilai budi daya ikan ini yang mendapatkan margin terbesar ada pada aktor pengepul, dimana pengepul yang mempunyai akses dan informasi dua arah, baik itu informasi pasar ataupun informasi mencari petani ikan.Kata kunci: budi daya ikan, rantai nilai, ikan air tawar, value-chain analysis (VCA

ANALISIS EKUITAS MEREK LAPTOP PADA MAHASISWA INSTITUT PERTANIAN BOGOR

Directory of Open Access Journals (Sweden)

Ujang Sumarwan

2010-08-01

Full Text Available The brand has role in bridging between consumer’s expectations and company’s promises. Prestigious brand can be said that it has strong brand equity. This research had objective to analyze brand equity of laptop product among students of Bogor Agricultural University. Meanwhile, this research had detail purposes are: (1 to analyze usage behavior of laptop product, (2 to analyze brand equity elements (brand awareness, brand association, perceived quality, and brand loyalty among students of Bogor Agricultural University, and (3 to find out the correlation between top of mind and laptop brand that used by students of Bogor Agricultural University. This research used cross sectional study design with total sample were 200 students. The determination of total samples in each faculty used proportional sampling. The process to select sample was executed by convenience sampling that was selection of  the samples based on availability to fill up the questionnaire and interview that appropriated with the quota in each faculty. The brand of laptop that most used and possessed is Acer. The most possessed of laptop by sample during 10 months until 18 months. The most way of buying laptop was bought by parents in new product. On the brand awareness elements of brand product, Acer generally get the better spot then followed by Toshiba, HP Compaq, and Axioo. The testing of brand association with using Cochran test showedthat the smallest association was obtained to Acer brand. It caused the other brand, such as Toshiba, HP Compaq, and Axioo hadall associations that adhere to consumers. The perceived quality that was tested by Customer Satisfaction Index (CSI, referred that index satisfaction of samples about Acer, Toshiba, and Axioo brand had satisfied level. Meanwhile, the samples that used HP Compaq had very satisfied level. The Importance Performance Analysis (IPA showed that only Toshiba brand that was the most excellent in which none atribute which

Analisis Kualitas Pelayanan di Restoran Saung Mirah, Bogor

OpenAIRE

Subakti, Agung Gita

2014-01-01

A restaurant is one of the facilities to carry out Food Service Industry or as a part tourism accommodation that plays a role in fulfilling the needs of tourists or customer. In this case, good service quality will determine the progress of a restaurant beside food and beverage as the offered products. Saung Mirah Restaurant Bogor is a restaurant that has a quality of service that is not as expected, thus causing a lot of complaints from guests. The purpose of this study is to look at the imp...

NVLAP activities at Department of Defense calibration laboratories

Energy Technology Data Exchange (ETDEWEB)

Schaeffer, D.M. [Defense Nuclear Agency, Alexandria, VA (United States)

1993-12-31

There are 367 active radiological instrument calibration laboratories within the U.S. Department of Defense (DoD). Each of the four services in DoD manages, operates, and certifies the technical proficiency and competency of those laboratories under their cognizance. Each service has designated secondary calibration laboratories to trace all calibration source standards to the National Institute of Standards and Technology. Individual service radiological calibration programs and capabilities, present and future, are described, as well as the measurement quality assurance (MQA) processes for their traceability. National Voluntary Laboratory Accreditation Program (NVLAP) programs for dosimetry systems are briefly summarized. Planned NVLAP accreditation of secondary laboratories is discussed in the context of current technical challenges and future efforts.

NVLAP activities at Department of Defense calibration laboratories

International Nuclear Information System (INIS)

Schaeffer, D.M.

1993-01-01

There are 367 active radiological instrument calibration laboratories within the U.S. Department of Defense (DoD). Each of the four services in DoD manages, operates, and certifies the technical proficiency and competency of those laboratories under their cognizance. Each service has designated secondary calibration laboratories to trace all calibration source standards to the National Institute of Standards and Technology. Individual service radiological calibration programs and capabilities, present and future, are described, as well as the measurement quality assurance (MQA) processes for their traceability. National Voluntary Laboratory Accreditation Program (NVLAP) programs for dosimetry systems are briefly summarized. Planned NVLAP accreditation of secondary laboratories is discussed in the context of current technical challenges and future efforts

PHYSICOCHEMICAL AND BACTERIOLOGICAL ANALYSIS OF ...

African Journals Online (AJOL)

cornerstone

Physiochemical and bacteriological quality of hand-dug well water in Zango – Abattoir Kaduna. Metropolis was ... concentration makes the water unsuitable for drinking. INTRODUCTION .... by disinfection or distillation so that bacteria and ...

Evaluasi Perubahan Penggunaan Lahan Kecamatan di Daerah Aliran Sungai Cisadane Kabupaten Bogor

Directory of Open Access Journals (Sweden)

Tessie Krisnaningtyas Endang Trimarmanti

2014-04-01

Full Text Available Bogor Regency is part of the National Strategic Area Jabodetabekpunjur, serves as the buffer area. However, it is not denied, accessibility making the Bogor Regency as one of the development center urban settlement and other economic activities. There are two Nature Conservation Area and seven Watershed, the greatest area is Cisadane Watershed, rich biodiversity, daily needs, but susceptible of land use change, especially high population area. The purpose study is to find out land use change and its influence factors on sub-district located in Cisadane Watershed, Bogor Regency. This study used mixed methodes research with series/multiply research, the first is spatial approach and quantitative analysis and the second is qualitative approach. Based on the discussion and findings research, the expansion development settlement in the suburban areas and productive lands convertion (Rustiadi et.al, 2003 happens in Cisadane watershed, urban activities have an impact transformation land use, agricultural into housing/settlement land was truly indeed. It had been occured before the provision of wetland agricultural area. Economic-social factors trigger developers the function of agricultural into housing with value added, no exception resident with the right ownership. Another factor is institutional factors, lack of commitment service from government to presence infrastructure and sustainability irrigation flow. The application of space utilization control has been conducted by sub- district and district officials through supervision and control accordance with the provisions of RTRW/IMB regulation, but this has offset by socialization to the public so a lot of society and officials government have yet know and understand the rules, so tendency land use change is quite high.

The history of creation and study of vitamin D medicines in the Laboratory of Medical Biochemistry of the Palladin Institute of Biochemistry of the NAS of Ukraine for 1990-2015

Directory of Open Access Journals (Sweden)

N. E. Lugovska

2015-12-01

Full Text Available The article presents the main results of inventive activity of the Laboratory of Medical Biochemistry headed by Dr. L. I. Apukhovska of the Palladin Institute of Biochemistry of the NAS of Ukraine. These researches continued the works initiated by Prof. V. P. Vendt and included development of technologies for the production of highly efficient preparations based on vitamin D3, namely “VIDEIN” in several modified forms (for prevention and treatment of rickets and rickets-like diseases in children, osteopathy of various origins, hypovitaminosis D in pregnant, mineral metabolism disorders, etc, water-soluble vitamin D3 (for prevention and treatment of rickets in children from the first months of life, “KALMIVID” and ”KALMIVID-M” (for treatment of bone tissue diseases associated with mineral metabolism disorders, the pharmaceutical composition “MEBIVID” (for treatment of osteoporosis and diseases associated with reduced bone mineral density and therapeutic vitamin-D3-E protein complex (for regulation of metabolic processes, improvement of structural and functional bone quality as well as structure and function of epiphyseal cartilage. All products are characterized by stability of vitamin D3 molecule, and thus, dosing accuracy and reliability, whilst not containing toxic preservatives and stabilizers. The technologies for production as well as analytical and normative documentations have been developed for all preparations.

Policies and practices in haemostasis testing among laboratories in Croatia: a survey on behalf of a Working Group for Laboratory Coagulation of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Science.gov (United States)

Bronić, Ana; Herak, Desiree Coen; Margetić, Sandra; Milić, Marija

2017-02-15

The objective of this survey was to assess current policies and practice in haemostasis testing among both hospital and outpatient laboratories in Republic of Croatia. A questionnaire with seventy questions divided into nine sections was created in May 2015. Participants were asked about their practice related to test request form, sample collection, prothrombin time (PT) and activated partial thromboplastin time assays, other individual haemostasis assays, point-of-care testing (POCT), reporting of coagulation tests results and quality assurance of procedures, the personnel and other laboratory resources, as well as on issues related to education and implementation of additional coagulation assays in their laboratory. The survey was administered and data were collected between June and September 2015. A total survey response rate was 104/170 (61.2%). Most respondents were faced with incomplete information on prescribed therapy and diagnosis on the test request or inappropriate samples withdrawn on distant locations, but also do not have protocols for handling samples with high haematocrit values. Reporting of PT-INR and D-dimer results was different between laboratories. Although almost all laboratories developed a critical value reporting system, reporting a value to general practitioners is still a problem. Result on coagulation POCT testing showed that not all devices were supervised by laboratories, which is not in compliance with Croatian Chamber of Medical Biochemistry acts. Obtained results highlighted areas that need improvement and different practice patterns in particular field of haemostasis testing among laboratories. A harmonization of the overall process of haemostasis testing at national level should be considered and undertaken.

[Standardization in laboratory hematology by participating in external quality assurance programs].

Science.gov (United States)

Nazor, Aida; Siftar, Zoran; Flegar-Mestrić, Zlata

2011-09-01

Since 1985, Department of Clinical Chemistry and Laboratory Medicine, Merkur University Hospital, Zagreb, has been participating in the International External Quality Assessment Scheme for Hematology (IEQAS-H) organized by the World Health Organization (WHO). Owing to very good results, in 1987 the Department received a certificate of participation in this control scheme. Department has been cooperating in the external quality assessment program in laboratory hematology which has been continuously performed in Croatia since 1986 by the Committee for External Quality Assessment Schemes under the auspices of the Croatian Society of Medical Biochemists and School of Pharmacy and Biochemistry, University of Zagreb. Nowadays, 186 medical biochemical laboratories are included in the National External Quality Assessment program, which is performed three times per year. Our Department has participated in the international projects of the European Committee for External Quality Assurance Programs in Laboratory Medicine (EQALM).

A program to develop the domestic natural gas industry in Indonesia: Case history of two World Bank projects

International Nuclear Information System (INIS)

Klass, D.L.; Khwaja, S.

1991-01-01

Indonesia depends heavily on revenues from the export of LNG and oil, the availability of which appears to be decreasing. It is therefore making a strong effort to accelerate development of a domestic natural gas industry. A high priority has been given to the conversion of power plants and city gas systems, including local industries and commercial facilities, from liquid fuels to natural gas. This will release more oil for export, help to meet the objectives of Repelita V, and provide substantial environmental benefits. The World Bank recently provided loans to the Indonesian Government for two projects that are aimed at substituting natural gas for oil and manufactured gas in domestic markets. One project involves expansion of the gas distribution systems of Indonesia's natural gas utility (PGN) in three cities: Jakarta and Bogor in Java, and Medan in Sumatra. The project also includes training programs for PGN staff and an energy pricing policy study to be carried out by Indonesia's Ministry of Mines and Energy. The second project involves expansion of the supply of natural gas for Surabaya and twelve other towns in its vicinity in East Java, and further expansion of Medan's supply system. Technical assistance will be provided to enhance the skills ofPGN and the Ministry of Mines and Energy, and a Gas Technology Unit similar to the Institute of Gas Technology will be established at Indonesia's Research and Development Center for Oil and Gas (LEMIGAS) in Jakarta. 14 refs., 3 figs., 11 tabs

TINGKAT KEPUASAN TERHADAP KUALITAS PELAYANAN PEMELIHARAAN DATA PENDAFTARAN TANAH DI KANTOR PERTANAHAN KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Gawil Despriyatmoko

2016-05-01

Full Text Available In order to support bureaucratic reform program in the field of land services, the National Land Registry Agency issued regulations on service standards and decisions about Sapta Land Code. This study aims to analyze the levels of interest and performance of the officials of Land Registry Office in Bogor Regency and to analyze the level of satisfaction of the community on the service of the maintenance of land registry data by the Office. The analytical tool used to determine the satisfaction of the community was the Importance Performance Analysis (IPA and Customer Satisfaction Index (CSI. The results showed that there is a gap between the petition of certification and completion of the certificate. The analysis results of IPA based on the dimensions of service quality maintenance of land registry data showed that overall the community is satisfied with the service quality of the Land Registry Office of Bogor Regency. This is demonstrated by the discrepancies between the performance average score of 3,54 and the interest average score of 3,49. Thus, the average score of a positive gap of 0,05 was obtained, and CSI value obtained was 71,14%. Based on the measurement of scale range using the Simamora formula, the CSI analytical results showed that the community is satisfied with the maintenance service of land records in the Land Registry Office of Bogor Regency.Keywords: satisfaction, service quality, maintenance of land registry data, IPA, CSI

[Bacteriological and clinical studies on flomoxef in the pediatric field].

Science.gov (United States)

Sunakawa, K; Ishizuka, Y; Kawai, N; Saito, N; Iwata, S; Sato, Y; Akita, H; Kusano, S; Aoki, T

1987-08-01

Bacteriological and clinical studies on flomoxef (FMOX, 6315-S) were performed and the results obtained are summarized below. 1. The MIC values of FMOX against 307 clinically isolated strains of Staphylococcus aureus were 0.024 to 100 micrograms/ml with a peak MIC of 0.39 microgram/ml, and the MIC90 value was 1.56 micrograms/ml. The MIC90 against methicillin resistant S. aureus (MRSA) was 25 micrograms/ml. 2. FMOX was administered to 15 children with pediatric bacterial infections, and the effectiveness was rated excellent or good in all cases. 3. In bacteriological evaluation, 7 of 11 strains identified prior to the treatment were eliminated (63.6%). 4. As side effects, diarrhea or soft stool was found in 3 cases and eruption in 1 case. As abnormal laboratory values, eosinophilia and thrombocytosis were found in 1 case each. 5. On the intestinal bacterial flora, FMOX had a marked influence just as did other Group 4 and 5 cephems antibiotics. 6. FMOX interfered little with the coagulation system or platelet aggregation.

Laboratory testing in the emergency department: an Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC and Academy of Emergency Medicine and Care (AcEMC consensus report

Directory of Open Access Journals (Sweden)

Giuseppe Lippi

2017-04-01

Full Text Available The mainstay of patient-oriented laboratory testing in emergency settings entails selecting number and type of tests according to valid criteria of appropriateness. Since the pattern of urgent tests requesting is variable across different institutions, we designed a joined survey between the Academy of Emergency Medicine and Care (AcEMC and the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC for reaching tentative consensus about the most informative diagnostic tests in emergency settings. A survey, containing the most commonly performed urgent laboratory tests and the relative clinical indications, was disseminated to eight relevant members of AcEMC and eight relevant members of SIBioC. All contributors were asked to provide numerical scores for the different laboratory parameters, where 1 indicated strongly recommended, 2 recommended in specific circumstances, and 3 strongly discouraged. The mean results of the survey were presented as the mean of responders’ values, and the parameters were finally classified as strongly recommended (mean value, 1.0-1.5, somehow recommended (mean value, 1.5-2.0, discouraged (mean value, 2.0-2.5 and strongly discouraged (mean value, 2.5-3.0. The results of the survey allowed defining a hierarchy of priority, wherein 24 tests were strongly recommended. The use of 5 common tests was instead strongly discouraged. For 16 additional parameters in the list, the consensus ranged between somehow recommended and discouraged. We hope that results presented in this joint AcEMC-SIBioC consensus document may help harmonizing panel of tests and requesting patters in emergency setting, at least at a national level.

Detection of Mycobacterium avium subspecies paratuberculosis in formula milk from Bogor using PCR IS 900

Directory of Open Access Journals (Sweden)

Widagdo S. Nugroho

2008-09-01

Full Text Available Crohn’s disease (CD that becomes a public health concern in developed countries shows similarities in clinical signs and pathological features with Johne’s disease (JD in ruminants infected by Mycobacterium avium subspecies paratuberculosis (MAP. Few researches conducted in Europe, the USA, and Australia showed relationships between MAP, CD, JD and dairy products. Indonesians consume milk and diary products from domestic and imported source. Adji in 2004 found some domestic dairy cows that were seropositive for MAP, and this could be a serious problem in dairy farm animals and human health in the future. The aim of this study was to detect MAP in the growing up formula milk. Fifty samples from five established factories were taken from supermarkets in Bogor. Polymerase chain reaction method (PCR with insertion sequence (IS 900 as primer and culture in Herrold’s egg yolk media with mycobactin J (HEYM J as a gold standard were used in this study. Neither MAP grew up in HEYM J medium after 20 weeks of culture period nor positive samples by PCR IS 900 were found. Although there were no positive samples found in this study, further extensive and comprehensive studies on MAP should be done with more and varied samples, as well as in human to provide data on MAP in Indonesia. (Med J Indones 2008; 17: 183-7Keywords: Crohn’s disease, dairy cow, growing up formula milk

‘Excuse me, sir. Please don’t smoke here’. A qualitative study of social enforcement of smoke-free policies in Indonesia

Science.gov (United States)

Kaufman, Michelle R; Merritt, Alice Payne; Rimbatmaja, Risang; Cohen, Joanna E

2015-01-01

Objective District policies were recently put into place in Indonesia prohibiting smoking in public spaces. This study sought to (1) assess participants’ general knowledge of secondhand smoke (SHS) dangers; (2) assess participants’ awareness of and specific knowledge of smoke-free (SF) policies; and (3) assess the extent to which such policies are socially enforced and gather examples of successful social enforcement. Methods Qualitative in-depth interviews and focus group discussions were conducted in Bogor and Palembang cities with both community members and key informants such as government officials, non-government agency staff, religious leaders and health workers. Results Participants in both Palembang and Bogor find SF policy important. Although there was awareness of SHS dangers and SF policies, accurate knowledge of the dangers and an in-depth understanding of the policies varied. There was a high level of support for the SF policies in both cities among both smokers and non-smokers. Many participants did have experience asking a smoker not to smoke in an area where it was restricted, even if their comfort in doing so varied. There was, however, a higher level of comfort in telling smokers to stop or to move away from pregnant women and children. Hesitation to socially enforce the policies was especially present when asking men of status and/or community leaders to stop smoking, but overall participants felt they could comfortably ask someone to obey the law. Conclusion Palembang and Bogor may be evolving towards creating social norms in support of prohibiting smoking in public spaces. If provided with more support from government and law officials, such as government officials themselves promoting the policies and demonstrating compliance, and renewed efforts to promote and enforce policies in general were made, Indonesians in these cities may feel more confident protecting non-smokers from SHS. PMID:25244917

PENGARUH IKLIM ORGANISASI TERHADAP PRODUKTIVITAS KERJA DI UNIT PELAKSANA TEKNIS KURIKULUM KECAMATAN JASINGA KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Ayi Karyana

2013-03-01

Full Text Available The research analyzed the influence of organizational climate dimension on the work productivity at the Local Technical Service Unit (UPTK for Pre-Elementary and Elementary Education in the Jasinga Sub District of Bogor District. The research used explanatory survey method. Purposive sampling was the technique used to obtained samples. Data were collected by means of questionnaires, interviews and observations. The questionnaires were given to 75 people at the UPTK and they were all returned. The method used to analyze the data is path analysis. Statistical result showed that the variable dimension of the organizational climate has positive and significant impact on the productivity. The UPTK in the Jasinga Sub District of Bogor was totality consistent in giving meaning to the nature of conducive organizational climate. But in order to increase the effectivity and efficiency of the work productivity, it is necessary to socialize the organizations vision and mission, to increase the intensity of the communication, to share responsibility on everybody, to motivate the human resource involved in the job, to encourage reward and punishment, and to provide technological facilities needed to support the organization. Penelitian ini menganalisis pengaruh dimensi iklim organisasi terhadap produktivitas kerja di Unit Pelaksana Teknis Kurikulum (UPTK Kecamatan Jasinga Kabupaten Bogor. Metode penelitian yang digunakan adalah metode survei eksplanatori. Teknik sampling yang digunakan adalah purposive sampling dengan pertimbangan dan bertujuan tertentu. Prosedur pengumpulan data dilakukan dengan penyebaran angket, wawancara dan observasi. Angket disebarkan kepada 75 orang pada UPTK Kecamatan Jasinga, dan angket kembali semuanya. Metode analisis data yang digunakan penulis adalah analisis jalur. Hasil pengujian statistik menunjukkan bahwa dimensi variabel iklim organisasi berpengaruh positif dan signifikan terhadap produktivitas kerja. UPTK Kecamatan Jasinga

Research programs at the Department of Energy National Laboratories. Volume 2: Laboratory matrix

Energy Technology Data Exchange (ETDEWEB)

NONE

1994-12-01

For nearly fifty years, the US national laboratories, under the direction of the Department of Energy, have maintained a tradition of outstanding scientific research and innovative technological development. With the end of the Cold War, their roles have undergone profound changes. Although many of their original priorities remain--stewardship of the nation`s nuclear stockpile, for example--pressing budget constraints and new federal mandates have altered their focus. Promotion of energy efficiency, environmental restoration, human health, and technology partnerships with the goal of enhancing US economic and technological competitiveness are key new priorities. The multiprogram national laboratories offer unparalleled expertise in meeting the challenge of changing priorities. This volume aims to demonstrate each laboratory`s uniqueness in applying this expertise. It describes the laboratories` activities in eleven broad areas of research that most or all share in common. Each section of this volume is devoted to a single laboratory. Those included are: Argonne National Laboratory; Brookhaven National Laboratory; Idaho National Engineering Laboratory; Lawrence Berkeley Laboratory; Lawrence Livermore National Laboratory; Los Alamos National Laboratory; National Renewable Energy Laboratory; Oak Ridge National Laboratory; Pacific Northwest Laboratory; and Sandia National Laboratories. The information in this volume was provided by the multiprogram national laboratories and compiled at Lawrence Berkeley Laboratory.

Attitudes of agricultural scientists in Indonesia towards genetically modified foods.

Science.gov (United States)

Februhartanty, Judhiastuty; Widyastuti, Tri Nisa; Iswarawanti, Dwi Nastiti

2007-01-01

Conflicting arguments and partial truths on genetically modified (GM) foods have left confusion. Although studies of consumer acceptance of GM foods are numerous, the study of scientists is limited. Therefore, the main objective of this study was to assess the attitudes of scientists towards GM foods. The study was a cross sectional study. A total of 400 scientists (involved in at least one of teaching, research and consultancy) in the Bogor Agricultural Institute, Indonesia were selected randomly from its faculties of agriculture, veterinary, fishery, animal husbandry, forestry, agricultural technology, mathematics and science, and the post graduate department. Data collection was done by face-to-face interview using a structured questionnaire and self-administered questionnaire. The result showed that the majority (72.8%) of the respondents were favorably disposed towards GM foods, 14.8% were neutral, and only 12.5% were against them. The majority (78.3%) stated that they would try GM food if offered. Most (71%) reported that they were aware of the term "GM foods". Only half of the respondents felt that they had a basic understanding about GM foods. However, based on a knowledge test, 69.8% had a good knowledge score. Nearly 50% indicated that they were more exposed to news which supported GM foods. Over 90% said that there should be some form of labeling to distinguish food containing GM ingredients from non-GM foods. Attitudes were significantly associated with willingness to try GM foods if offered, restrictions on GM foods, and exposure to media reports about the pros and cons of GM foods.

A biochemistry discipline designed for the nutrition course

Directory of Open Access Journals (Sweden)

A.A.G. Bianco

2004-05-01

Full Text Available Biochemistry is widely considered an essential background in a Nutrition Course framework. At theFaculdade de Saude Publica, USP, it is a direct requirement to eight disciplines of the syllabus and anindirect requirement to another nine disciplines. Nevertheless, a previous interview study involvingNutrition students and Nutritionists revealed a contradictory image of Biochemistry. Although stu-dents and Nutritionists admitted the important role played by Biochemistry, most of the respondentsdeclared that they could not foresee any application of Biochemical contents in their professional life.Aiming to change this situation, a deep intervention in the Biochemistry discipline was carried on.The discipline was planned in such a way that all the contents to be taught was directly derived fromsubjects or situations matching the interests of nutrition students. Instead of a classical lecture basedcourse, collaborative learning was the methodological choice, taking advantage of practical activitiesinvolving educational software and laboratory work as well. The course was carried on in 180 hoursand a variety of strategies were employed, especially small group discussion and problem solving. Thestudents were given a booklet containing all the exercises and problems, which acted as course guide.At the end of the course, an evaluation survey was carried out. It is noticeable that, according tostudents answers: 100% agreed that Biochemistry was intimately linked to Nutrition; 83% appreciatedthe didactical methodologies employed; 89% would like to continue studying Biochemistry in a furtherdiscipline; 96% declared that the discipline has raised their interest in Biochemistry. In respect tothe assessment of the students, these results are in accordance with the opinion of teachers and TAsengaged in restructuring Biochemistry courses.

Bacteriological Quality of Dried Sliced Beef (Kilishi) Sold In Ilorin ...

African Journals Online (AJOL)

DR. MIKE HORSFALL

ABSTRACT: The bacteriological quality of dried sliced beef (kilishi) obtained from three selling points in. Ilorin metropolis was determined in order to ascertain its safety. The total bacterial count, Enterobacteriaceae count, Staphylococcus aureus count and E.coli counts were used as index of bacteriological quality. Samples.

Clinical, Bacteriologic, and Geographic Stratification of Melioidosis Emerges from the Sri Lankan National Surveillance Program.

Science.gov (United States)

Sathkumara, Harindra D; Merritt, Adam J; Corea, Enoka M; Krishnananthasivam, Shivankari; Natesan, Mohan; Inglis, Timothy J J; De Silva, Aruna Dharshan

2018-02-01

Melioidosis, a potentially fatal tropical infection, is said to be underdiagnosed in low-income countries. An increase in melioidosis cases in Sri Lanka allowed us to analyze the relationship among clinical outcome, bacteriology, epidemiology, and geography in the first 108 laboratory-confirmed cases of melioidosis from a nationwide surveillance program. The additional 76 cases of laboratory-confirmed melioidosis confirmed further associations between Burkholderia pseudomallei multilocus sequence typing (MLST) and infection phenotype; ST1137/unifocal bacteremic infection (χ 2 = 3.86, P national genotyping-supported melioidosis registry will improve melioidosis diagnosis, treatment, and prevention where underdiagnosis and mortality rates remain high.

Kejadian Hujan Asam di Kabupaten Bogor dan Retensi Timbal pada Domba Lokal yang Diberi Ransum Berkadar Timbal Tinggi

Directory of Open Access Journals (Sweden)

D. Diapari

2008-12-01

Full Text Available The objectives of this research were: 1 to find out whether acid rain has taken place in Bogor Regency area and its effect on the lead (Pb content in soil and roughage in the region, 2 to predict the correlation of Pb content in rain water and soil, and the content of Pb in roughage in the Bogor regency area, 3 to study the impact of acid and Pb content in the ration to sheep daily gain and measure Pb content in liver, kidney and meat. The results showed that in Bogor regency area the acid rain occurred during the dry season but not in the wet season. The Pb content in the rainwater and soil were not affected by acid rain. There was no correlation of Pb content between soil and roughage. Acid containing ration decreased daily gain, but not for the ration of high Pb-content. Acid containing ration increased Pb content in the kidney but not in the liver and meat. However, high Pb content in the ration increased the Pb content in the liver and kidney but not in the meat.

KONSUMSI MAKANAN DI DESA TERTINGGAL WILAYAH BOGOR, TANGERANG DAN BEKASI (BOTABEK SEBAGAI DAMPAK KRISIS EKONOMI

Directory of Open Access Journals (Sweden)

Djoko Kartono

2012-11-01

Full Text Available FOOD CONSUMPTION IN POOR VILLAGES OF BOGOR, TANGERANG AND BEKASI REGIONS AS THE IMPACT OF ECONOMIC CRISIS.Background: Socio-economic condition of population was deteriorated by economic crisis that hit Indonesia in mid of 1997 altogether with long wry season during 1997. Economic factors such as increase in the price of the daily needs, reduction in agricultural production and the level of income in rural areas, contraction in the construction sector in urban areas, depression in the manpower market altogether had negative impact to the welfare of the lower income population.Objective: The aim of the survey was to learn the nutrient intake of household in poor villages as the impact of economic crisis.Method: The Nutritional Consumption Survey in all villages of West Java Province was conducted at the end of 1996 and these data were used as baseline. Similar survey was conducted at the end of 1999 in Bogor City, Tangerang and Bekasi Districts as the follow-up data. Thirty villages in each district were selected randomly to include 10 poor villages and 20 non-poor category villages. In each village, one Posyandu that had been the location of 1996 survey was selected. Ten households that have under-five child were selected randomly in the Posyandu.Results: Household. Energy consumption in poor villages and non-poor category villages were higher before the crisis compared to during the crisis. Protein consumption in the non-poor villages before the crisis was similar to during crisis. Vitamin A consumption in non-poor and poor villages much lower during crisis compared to before crisis. Under-five children 1999. Energy, protein and fat intake of under-five children was similar between poor and non-poor category villages. Carbohydrate intake was significantly different between poor and non-poor category villages. Iron intake was similar before and during crisis.Conclusions: Economic crisis has negative impact on energy and protein consumption both

Combining content and elements of communication into an upper-level biochemistry course.

Science.gov (United States)

Whittington, Carli P; Pellock, Samuel J; Cunningham, Rebecca L; Cox, James R

2014-01-01

This report describes how a science communication module was incorporated into an advanced biochemistry course. Elements of communication were taught synergistically with biochemistry content in this course in an effort to expose students to a variety of effective oral communication strategies. Students were trained to use these established techniques and incorporated them into various presentations throughout the course. Three students describe their use of specific resources and how the skills learned relate to their future career. The importance and relevance of science communication are receiving unprecedented national attention. The academic scientific community must respond by incorporating more communication-centered instruction and opportunities in the classroom and laboratory. © 2013 by The International Union of Biochemistry and Molecular Biology.

Alternative futures for the Department of Energy National Laboratories

Energy Technology Data Exchange (ETDEWEB)

1995-02-01

This Task Force was asked to propose alternate futures for the Department of Energy laboratories noted in the report. The authors` intensive ten months` study revealed multiple missions and sub-missions--traditional missions and new missions--programs and projects--each with factors of merit. They respectively suggest that the essence of what the Department, and particularly the laboratories, should and do stand for: the energy agenda. Under the overarching energy agenda--the labs serving the energy opportunities--they comment on their national security role, the all important energy role, all related environmental roles, the science and engineering underpinning for all the above, a focused economic role, and conclude with governance/organization change recommendations.

Pocket Checklists of Indonesian timber trees

NARCIS (Netherlands)

Prawira, Soewanda A.; Tantra, I.G.M.; Whitmore, T.C.

1984-01-01

Indonesia as yet does not have a comprehensive account of the forest trees which reach timber size (35 cm dbh = 14 inch or 105 cm gbh = 42 inch). A project has been started in August 1983 by the Botany Section of the Forest Research Institute in Bogor, Indonesia, to prepare pocket checklists of the

Human factors at the Department of Energy National Laboratories

International Nuclear Information System (INIS)

Pond, D.J.; Waters, R.M.

1991-01-01

After World War II, a system of national laboratories was created to foster a suitable environment for scientific research. This paper reports that today, human factors activities are in evidence at most of the nine U.S. Department of Energy multi-program national laboratories as well as at a number of special program facilities. This paper provides historical and future perspectives on the DOE's human factors programs

Retraction 2 | Shafik | Egyptian Journal of Biochemistry and ...

African Journals Online (AJOL)

Value of co-peptin/ plasminogen activator inhibitor-1 axis in early diagnosis of preterm labor risk among pre-eclamptic Egyptian women. Noha M. Shafik1, Soha S. Zakaria1, Ahmed M. Hagras2 and Ghada M. Abou-Fard3 Departments of Medical Biochemistry1, Gynecology2 and Physiology3, Faculty of Medicine, Tanta ...

History of Biochemistry at the University of Geneva From the Boulevard des Philosophes to Quai Ernest-Ansermet.

Science.gov (United States)

Deshusses, Jacques; Riezman, Howard

2009-12-01

A brief account of the developments in biochemistry at the Faculty of Science of the University of Geneva is given from its emergence from organic chemistry at the Ancienne Ecole de chimie to today's Department of Biochemistry at the Section de chimie et biochimie.

Bioekologi vektor demam berdarah dengue (DBD serta deteksi virus dengue pada Aedes aegypti (Linnaeus dan Ae. albopictus (Skuse (Diptera: Culicidae di kelurahan endemik DBD Bantarjati, Kota Bogor

Directory of Open Access Journals (Sweden)

Zahara Fadilla

2015-09-01

Full Text Available Dengue hemorrhagic fever (DHF is a viral disease that threatened community health in Indonesia. As part of an eradication program, it is important to learn the behavioral aspect of the disease vector. The aims of this study were to detect the presence of dengue virus in Aedes spp., at Bantarjati Village, Bogor City and to learn to bioecology of. Aedes aegypti (Linnaeus. Detection of dengue virus in Aedes spp. were done by reverse transcription-polymerase chain reaction (RT-PCR technique that consist of two phase were synthesis phase and cDNA amplification and dengue virus serotipe characterization. The Ae. aegypti and Ae. albopictus (Skuse mosquitoes were collected using the landing and resting moquito collection technique booth indoors and outdoors. The highest density of Ae. aegypti and Ae. albopictus were found in April and the peak activity was occurred at 10:00-11:00 am. Dengue virus was not detected in female mosquitoes Aedes spp.

78 FR 12323 - Announcement of the Re-Approval of the Commission on Office Laboratory Accreditation (COLA) as an...

Science.gov (United States)

2013-02-22

... subspecialty areas under CLIA: Microbiology, including Bacteriology, Mycobacteriology, Mycology, Parasitology... CLIA for the following specialties and subspecialties: Microbiology, including Bacteriology... requirements. The COLA requires the laboratory director to review quality control results for waived tests...

INDUSTRI KREATIF INDONESIA: PENDEKATAN ANALISIS KINERJA INDUSTRI

OpenAIRE

Ahmad Kamil

2015-01-01

In 2008, the Department of Commerce of the Republic of Indonesia has launched a creative economic development documents interpreted the 2025 Indonesia became the starting point and guide the development of the creative economy in Indonesia. With the existence of this document, the industry and its stakeholders or other stakeholders can readily develop the creative economy in Indonesia. Economic development in the direction of the creative industries is one manifestation of optimism aspiration...

Inventive activity of the Department of Protein Structure and Function of the Palladin Institute of Biochemistry of NAS of Ukraine. Part I. Development of the diagnostic methods for detection of hemostasis disorders and characterization of certain blood coagulation factors

Directory of Open Access Journals (Sweden)

V. M. Danilova

2016-04-01

Full Text Available The practical aspects of inventive activity of the Department of Protein Structure and Function of the Palladin Institute of Biochemistry, NAS of Ukraine are highlighted in this article. Through years of fundamental and applied researches of blood coagulation system proteins, initiated by luminaries of the world biochemistry O. V. Palladin and V. O. Belitser, the Department staff have developed a considerable number of methods, techniques and tests for the assessment of the state of the hemostasis system, which were approved in many clinics. In the first part of this work the authors describe the development of the diagnostic methods for identifying the homeostasis system disorders in detail, as well as characterize certain coagulation factors.

A Two-Week Guided Inquiry Protein Separation and Detection Experiment for Undergraduate Biochemistry

Science.gov (United States)

Carolan, James P.; Nolta, Kathleen V.

2016-01-01

A laboratory experiment for teaching protein separation and detection in an undergraduate biochemistry laboratory course is described. This experiment, performed in two, 4 h laboratory periods, incorporates guided inquiry principles to introduce students to the concepts behind and difficulties of protein purification. After using size-exclusion…

DOE standard: The Department of Energy Laboratory Accreditation Program for radiobioassay

International Nuclear Information System (INIS)

1998-12-01

This technical standard describes the US Department of Energy Laboratory Accreditation Program (DOELAP) for Radiobioassay, for use by the US Department of Energy (DOE) and DOE Contractor radiobioassay programs. This standard is intended to be used in conjunction with the general administrative technical standard that describes the overall DOELAP accreditation process--DOE-STD-1111-98, Department of Energy Laboratory Accreditation Program Administration. This technical standard pertains to radiobioassay service laboratories that provide either direct or indirect (in vivo or in vitro) radiobioassay measurements in support of internal dosimetry programs at DOE facilities or for DOE and DOE contractors. Similar technical standards have been developed for other DOELAP dosimetry programs. This program consists of providing an accreditation to DOE radiobioassay programs based on successful completion of a performance-testing process and an on-site evaluation by technical experts. This standard describes the technical requirements and processes specific to the DOELAP Radiobioassay Accreditation Program as required by 10 CFR 835 and as specified generically in DOE-STD-1111-98

'Excuse me, sir. Please don't smoke here'. A qualitative study of social enforcement of smoke-free policies in Indonesia.

Science.gov (United States)

Kaufman, Michelle R; Merritt, Alice Payne; Rimbatmaja, Risang; Cohen, Joanna E

2015-10-01

District policies were recently put into place in Indonesia prohibiting smoking in public spaces. This study sought to (1) assess participants' general knowledge of secondhand smoke (SHS) dangers; (2) assess participants' awareness of and specific knowledge of smoke-free (SF) policies; and (3) assess the extent to which such policies are socially enforced and gather examples of successful social enforcement. Qualitative in-depth interviews and focus group discussions were conducted in Bogor and Palembang cities with both community members and key informants such as government officials, non-government agency staff, religious leaders and health workers. Participants in both Palembang and Bogor find SF policy important. Although there was awareness of SHS dangers and SF policies, accurate knowledge of the dangers and an in-depth understanding of the policies varied. There was a high level of support for the SF policies in both cities among both smokers and non-smokers. Many participants did have experience asking a smoker not to smoke in an area where it was restricted, even if their comfort in doing so varied. There was, however, a higher level of comfort in telling smokers to stop or to move away from pregnant women and children. Hesitation to socially enforce the policies was especially present when asking men of status and/or community leaders to stop smoking, but overall participants felt they could comfortably ask someone to obey the law. Palembang and Bogor may be evolving towards creating social norms in support of prohibiting smoking in public spaces. If provided with more support from government and law officials, such as government officials themselves promoting the policies and demonstrating compliance, and renewed efforts to promote and enforce policies in general were made, Indonesians in these cities may feel more confident protecting non-smokers from SHS. Published by Oxford University Press in association with The London School of Hygiene and

Bacteriological studies off Mangalore coast

Digital Repository Service at National Institute of Oceanography (India)

Nair, S.; LokaBharathi, P.A.

Bacteriological studies of sediment and water in the near shore region off Mangalore showed that both limnotolerant and halotolerant bacteria were present. Significant inter-correlation (quantitative and qualitative) could be noted between various...

ESTIMASI KERUGIAN EKONOMI AKIBAT STATUS GIZI BURUK DAN BIAYA PENANGGULANGANNYA PADA BALITA DI BERBAGAI PROVINSI DI INDONESIA

Directory of Open Access Journals (Sweden)

Muhammad Aries

2012-02-01

Full Text Available 800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 st1\\:*{behavior:url(#ieooui } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} The study was aimed to estimate  GDP lost due to Protein Energy Malnutrition (PEM among under five children at various provinces in Indonesia. It was a descriptive study used secondary data.  Data analysis was conducted in Bogor, from January to March 2006. The data uses are prevalence of PEM among under five children in various provinces in 2003, Gross Regional Domestic Product (GRDP of provinces in Indonesia by industrial origin 2000 - 2004, population by province, sex and age group 2003, composite Consumers Price Index (CPI of 45 cities (2002 = 100. The study showed that the economic lost due to malnutrition (PEM among under five children in Indonesia was ranged from 0.27% to 1.21% GDP. Keyword: economic lost, Protein Energy Malnutrition, cost of food supplementation.

Laboratory of Cell and Molecular Biology

Data.gov (United States)

Federal Laboratory Consortium — The Laboratory of Cell and Molecular Biology investigates the organization, compartmentalization, and biochemistry of eukaryotic cells and the pathology associated...

Bacteriological diagnosis and molecular strain typing of Mycobacterium bovis and Mycobacterium caprae.

Science.gov (United States)

Gormley, E; Corner, L A L; Costello, E; Rodriguez-Campos, S

2014-10-01

The primary isolation of a Mycobacterium sp. of the Mycobacterium tuberculosis complex from an infected animal provides a definitive diagnosis of tuberculosis. However, as Mycobacterium bovis and Mycobacterium caprae are difficult to isolate, particularly for animals in the early stages of disease, success is dependent on the optimal performance of all aspects of the bacteriological process, from the initial choice of tissue samples at post-mortem examination or clinical samples, to the type of media and conditions used to cultivate the microorganism. Each step has its own performance characteristics, which can contribute to sensitivity and specificity of the procedure, and may need to be optimized in order to achieve the gold standard diagnosis. Having isolated the slow-growing mycobacteria, species identification and fine resolution strain typing are keys to understanding the epidemiology of the disease and to devise strategies to limit transmission of infection. New technologies have emerged that can now even discriminate different isolates from the same animal. In this review we highlight the key factors that contribute to the accuracy of bacteriological diagnosis of M. bovis and M. caprae, and describe the development of advanced genotyping techniques that are increasingly used in diagnostic laboratories for the purpose of supporting detailed epidemiological investigations. Copyright © 2014 Elsevier Ltd. All rights reserved.

Effectiveness of Ivermectin and Albendazole against Haemonchus contortus in Sheep in West Java, Indonesia.

Science.gov (United States)

Puspitasari, Silvia; Farajallah, Achmad; Sulistiawati, Erni; Muladno

2016-02-01

Administering a half dose of an anthelmintic is a simple method for detecting resistance in parasites infesting small ruminants. When a single anthelmintic fails in native sheep from Indonesia, a combination of anthelmintics from different chemical classes with different modes of action are administered as an alternative parasite-control strategy. This study compared the anthelmintic efficacy of ivermectin (IVM) and albendazole (ABZ) given either separately as a single dose or half dose or co-administered to sheep naturally infected with Haemonchus contortus. Twelve sheep from Bogor, West Java, Indonesia were divided into the following six treatment groups: half-dose IVM, full-dose IVM, half-dose ABZ, full-dose ABZ, combined IVM + ABZ, and control. The treatment efficacy was determined using the faecal egg count reduction test (FECRT) at day 0 (pre-treatment) and post-treatment at days 7, 14, 21, 28, 35, and 42. The efficacies of half-dose IVM, full-dose IVM, half-dose ABZ, full-dose ABZ, and the combination treatment ranged from -1900% to 100%, 99% to 100%, -167% to 100%, -467% to 89%, and -200% to 100%, respectively. The FECRT for the half-dose IVM, half-dose ABZ, full-dose ABZ showed that H. contortus is resistant to half-dose IVM and ABZ. Full-dose IVM was effective against H. contortus. The combined treatment was more effective against H. contortus than ABZ alone.

An Integrated Approach to Teaching Biochemistry for Pharmacy Students.

Science.gov (United States)

Poirier, Therese I.; Borke, Mitchell L.

1982-01-01

A Duquesne course integrating biochemistry lectures, clinical applications lectures, and laboratory sessions has the objectives of (1) making the course more relevant to students' perceived needs; (2) enhancing the learning process; (3) introducing clinical applications early in the students' program; and (4) demonstrating additional…

Bacteriology of drinking water distribution systems: an integral and multidimensional review.

Science.gov (United States)

Liu, G; Verberk, J Q J C; Van Dijk, J C

2013-11-01

A drinking water distribution system (DWDS) is the final and essential step to supply safe and high-quality drinking water to customers. Biological processes, such as biofilm formation and detachment, microbial growth in bulk water, and the formation of loose deposits, may occur. These processes will lead to deterioration of the water quality during distribution. In extreme conditions, pathogens and opportunistic pathogens may proliferate and pose a health risk to consumers. It is, therefore, necessary to understand the bacteriology of DWDSs to develop effective strategies that can ensure the water quality at consumers' taps. The bacteriology of DWDSs, both the quantitative growth and the qualitative bacterial community, has attracted considerable research attention. However, the researchers have focused mainly on the pipe wall biofilm. In this review, DWDS bacteriology has been reviewed multidimensionally, including both the bacterial quantification and identification. For the first time, the available literature was reviewed with an emphasis on the subdivision of DWDS into four phases: bulk water, suspended solids, loose deposits, and pipe wall biofilm. Special concentration has been given to potential contribution of particulate matter: suspended particles and loose deposits. Two highlighted questions were reviewed and discussed: (1) where does most of the growth occur? And (2) what is the contribution of particle-associated bacteria to DWDS bacteriology and ecology? At the end of this review, recommendations were given based on the conclusion of this review to better understand the integral DWDS bacteriology.

[Contribution to the establishment of quality assurance in five medical microbiology departments in Togo].

Science.gov (United States)

Katawa, G; Kpotsra, A; Karou, D S; Eklou, M; Tayi, K E; de Souza, C

2011-02-01

In Togo, as in many other developing countries, there is a lack of data on quality control and assurance of laboratories. The present study aimed to access for the quality management system in five medical bacteriology laboratories in Togo. The study was conducted from May to August 2006. Data were recorded by an audit on the reliability of results and the technical organization of laboratories. The standard ISO 15189:2003, the Togolese guide of good laboratory practices (GBEA-Togo) and the WHO medical bacteriology standards were used as references. The results of the audit showed a lack of culture media in laboratories, inappropriate choice of culture media, partial identification of some microorganisms, variability of identification procedures, a lack of diagnostic reagents and an inability to identify some potentially pathogenic bacteria. Concerning the technical organization of laboratories, compliance average ranging from 25.8 to 54.8 % was recorded. This indicates a limited organization of such laboratories. The issue of this study showed that laboratories must be equipped, their technical organization should be improved and they must establish a program of equipment maintenance.

Laboratory and field evaluation of spatial repellency with metofluthrin impregnated paper strip against mosquitoes in Lombok Island, Indonesia.

OpenAIRE

Kawada, Hitoshi; Maekawa, Yoshihide; Tsuda, yoshio; Takagi, Masahiro

2004-01-01

Spatial repellency of a new multilayer paper strip impregnated with metofluthrin, a newly synthesized pyrethroid, was evaluated in the laboratory and in the field at Kerandangan, Lombok Island, Indonesia,with the use of cow- and human-baited double nets. Spatial repellency was observed in both cow- and human-baited collections. Metofluthrin treatment reduced mosquito collection by >8O% during the lst 4 weeks.However, repellency seemed to reduce with the loss of metofluthrin by evaporation wit...

PENGARUH MEDIA KAMPANYE SARAPAN SEHAT TERHADAP PERUBAHAN PENGETAHUAN, SIKAP, DAN KEBIASAAN SARAPAN ANAK SEKOLAH DASAR DI KABUPATEN BOGOR

Directory of Open Access Journals (Sweden)

Dodik Briawan

2013-11-01

Full Text Available ABSTRACTThe purpose of this study was to analyze the effect of a healthy breakfast campaign on the changes in knowledge, attitudes, and breakfast habits among elementary school children in Bogor District. A quasy experimental study was applied to this study. Subjects in this study were 229 elementary school children in the nine villages in Bogor District. Nutrition education media used in this study were power point, puppets, picture cards, and drama. The school childrens knowledge and attitude showed that there was a significant difference between media intervention of power point, puppet, and drama to picture cards. The highest scores of knowledge (73.23±1.14 and attitude (91.84±1.09 was in picture cards. The percentage of subjects having a habit of breakfast before intervention was 78.2% and increased to 81.7% after intervention. Media intervention by picture cards gave highest change to increased breakfast knowledge, attitude, and habits for elementary school children.Keywords: breakfast, elementary school children, nutritional educationABSTRAKTujuan dari penelitian ini adalah untuk menganalisis pengaruh kampanye sarapan sehat terhadap perubahan pengetahuan, sikap, dan kebiasaan sarapan anak sekolah dasar di Kabupaten Bogor. Desain yang digunakan dalam penelitian ini adalah studi eksperimental semu. Subjek penelitian adalah anak sekolah dasar berjumlah 229 siswa dari sembilan desa di Kabupaten Bogor. Media pendidikan gizi yang digunakan adalah power point, wayang, kartu bergambar, dan drama. Hasil analisis ragam menunjukkan bahwa jenis media kampanye berpengaruh nyata terhadap skor pengetahuan dan sikap anak SD terhadap sarapan. Media kartu bergambar mempunyai skor pengetahuan dan sikap tentang sarapan sehat tertinggi yaitu masing-masing sebesar 73.23±1.14 dan 91.84±1.09 berbeda nyata dengan media lainnya. Persentase subjek yang memiliki kebiasaan sarapan sebelum intervensi sebesar 78.2% dan meningkat menjadi sebesar 81.7% setelah

Cerebrospinal fluid protein and glucose examinations and tuberculosis:
Will laboratory safety regulations force a change of practice?

Science.gov (United States)

Tormey, William P; O'Hagan, Christopher

2015-01-01

Cerebrospinal fluid (CSF) protein and glucose examinations are usually performed in chemical pathology departments on autoanalysers. Tuberculosis (TB) is a group 3 biological agent under Directive 2000/54/EC of the European Parliament but in the biochemistry laboratory, no extra precautions are taken in its analysis in possible TB cases. The issue of laboratory practice and safety in the biochemical analyses of CSF specimens, when tuberculosis infection is in question is addressed in the context of ambiguity in the implementation of current national and international health and safety regulations. Additional protective measures for laboratory staff during the analysis of CSF TB samples should force a change in current laboratory practice and become a regulatory issue under ISO 15189. Annual Mantoux skin test or an interferon-γ release assay for TB should be mandatory for relevant staff. This manuscript addresses the issue of biochemistry laboratory practice and safety in the biochemical analyses of CSF specimens when tuberculosis infection is in question in the context of the ambiguity of statutory health and safety regulations.

Bacteriologically confirmed tuberculosis in children

International Nuclear Information System (INIS)

Ozere, I.; Sele, A.; Ozolina, A.

2005-01-01

Tuberculosis in children and adults is a growing problem with important public health implications. In Latvia the incidence of tuberculosis (TB) in children up to age 14 has increased from 7,1 per 100000 in 1992 to 28,8 per 100000 in 2003. The diagnosis of TB is confirmed by isolation and identification of M. tuberculosis (MT) from clinical specimen. Confirmation of the disease, however, is difficult in children due to poor bacilli excretion and even under the best circumstances only about 30-40% of pediatric TB cases are proved bacteriologically. Of the 370 pediatric TB cases diagnosed between January 1, 2001 and December 1, 2003 in Latvia, 53 (14,3%) were confirmed bacteriologically. The clinical, radiological, immunological and anamnestic features of confirmed TB can serve as cornerstones for diagnosing of TB, when culture is not available. Objective To evaluate the sensitivity of diagnostic criteria of TB, clinical and radiological manifestation of TB and drug susceptibility of MT isolated also. Methods All consecutive children (53 in total) up to age 14 diagnosed with bacteriologically confirmed TB during 01.01.2001. -01.12.2003. were prospectively evaluated for reasons mentioned above. Results Of the 53 children identified all but one had respiratory tract TB. 17(32,1 %) children were under 4 years of age, 9 (17%) children were 5-9 years old, but 27 (50,9%) patients were 10-14 years old. During evaluation data on TB source case were found in addition in 13 children and total TB contact history was positive in 37 (69,8%) patients. All clinical and radiographical forms of respiratory tract TB were diagnosed. The most common encountered forms were intrathoracic adenopathy in 10 (18,9%) cases and TB pneumonia in 6 (11,3%) cases in children aged 10-14 years. lnthrathoracic adenopathy associated with segmental parenchymal lesion was the most common form in children under 4 years of age -7 (13,2%) cases respectively. Conclusions 1. The clinical and radiological

A Development Strategy for Dairy Goat Farms in Bogor Regency - West Java

OpenAIRE

Cyrilla, L; Purwanto, B. P; Astuti, D. A; Atabany, A; Sukmawati, A

2016-01-01

The objectives of present study were to formulate development strategies for dairy goat' farms that capable of supporting goat's milk agribusiness. Three medium scale dairy goat farms located in Bogor Regency which has population of more than 100 heads and has established more than 10 years were purposively selected for the study. Data were collected during February to May 2014. The design of this study was based on descriptive qualitative approach (rapid appraisal approach). Data were analiz...

Laboratory and field evaluation of spatial repellency with metofluthrin-impregnated paper strip against mosquitoes in Lombok Island, Indonesia.

Science.gov (United States)

Kawada, Hitoshi; Maekawa, Yoshihide; Tsuda, Yoshio; Takagi, Masahiro

2004-09-01

Spatial repellency of a new multilayer paper strip impregnated with metofluthrin, a newly synthesized pyrethroid, was evaluated in the laboratory and in the field at Kerandangan, Lombok Island, Indonesia, with the use of cow- and human-baited double nets. Spatial repellency was observed in both cow- and human-baited collections. Metofluthrin treatment reduced mosquito collection by >80% during the 1st 4 weeks. However, repellency seemed to reduce with the loss of metofluthrin by evaporation within 6 wk after treatment.

Blood gas testing and related measurements: National recommendations on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Science.gov (United States)

Dukić, Lora; Kopčinović, Lara Milevoj; Dorotić, Adrijana; Baršić, Ivana

2016-10-15

Blood gas analysis (BGA) is exposed to risks of errors caused by improper sampling, transport and storage conditions. The Clinical and Laboratory Standards Institute (CLSI) generated documents with recommendations for avoidance of potential errors caused by sample mishandling. Two main documents related to BGA issued by the CLSI are GP43-A4 (former H11-A4) Procedures for the collection of arterial blood specimens; approved standard - fourth edition, and C46-A2 Blood gas and pH analysis and related measurements; approved guideline - second edition. Practices related to processing of blood gas samples are not standardized in the Republic of Croatia. Each institution has its own protocol for ordering, collection and analysis of blood gases. Although many laboratories use state of the art analyzers, still many preanalytical procedures remain unchanged. The objective of the Croatian Society of Medical Biochemistry and Laboratory Medicine (CSMBLM) is to standardize the procedures for BGA based on CLSI recommendations. The Working Group for Blood Gas Testing as part of the Committee for the Scientific Professional Development of the CSMBLM prepared a set of recommended protocols for sampling, transport, storage and processing of blood gas samples based on relevant CLSI documents, relevant literature search and on the results of Croatian survey study on practices and policies in acid-base testing. Recommendations are intended for laboratory professionals and all healthcare workers involved in blood gas processing.

Agricultural production - Phase 2. Indonesia. Implementation of ELISA for brucellosis at DGLS laboratories in indonesia

International Nuclear Information System (INIS)

Patten, B.

1992-01-01

This expert mission was performed to assess the current status of activities relating to brucellosis testing at the Regional Disease Investigation Centres of the Directorate General of Livestock Services in Indonesia, and to assist in the establishment and validation of the ELISA technique for detecting Brucella antibodies

Bacteriological profile of neonatal septicemia in a tertiary care hospital from Western India

Directory of Open Access Journals (Sweden)

Vrishali Avinash Muley

2015-01-01

Full Text Available Neonatal septicemia is an important cause of morbidity and mortality. The present study was undertaken to determine the bacteriological profile and antimicrobial susceptibility pattern of prevalent pathogens isolated from the blood of septicemic neonates from Neonatal Intensive Care Unit (NICU. A total of 180 blood samples of septicemic neonates were studied bacteriologically. Antimicrobial susceptibility testing was done by the Kirby Bauer disc diffusion method in accordance to Clinical Laboratory Standards Institutes (CLSI guidelines. 26.6% (48 out of 180 cases of septicemia could be confirmed by blood culture. Of these, 66.7% cases were of early onset septicemia (EOS and 33.3% were of late onset septicemia (LOS. Klebsiella pneumoniae was the predominant pathogen (35.4% among the Gram-negative pathogens and Staphylococcus aureus (22.9% was the predominant Gram-positive pathogen. 28% of K. pneumoniae and E. coli isolates were extended spectrum beta-lactamase (ESBL producers. 18.1% of the Staphylococcus isolates were methicillin-resistant S. aureus (MRSA. Multi-drug-resistance pattern was observed with all the isolates. Ciprofloxacin and aminoglycosides were the most effective drugs against Gram-positive and Gram-negative isolates. This study highlights the predominance of Gram-negative organisms in causing neonatal sepsis and emergence of multi-drug-resistant strains in our set up.

Clinical characteristics differentiating bacteriologically positive pulmonary tuberculosis patients from negative ones in Mongolia.

Science.gov (United States)

Toyota, M; Yasuda, N; Koda, S; Ohara, H; Enkhbat, S; Tsogt, G

1998-06-01

The objective of this study is to clarify clinical characteristics which differentiate bacteriologically positive pulmonary tuberculosis patients from negative ones in Mongolia. The subjects include 338 patients aged 16 years and older who had undergone bacteriological examinations. Of them, 107 patients (31.7%) were confirmed bacteriologically. The proportion of bacteriological positive results increased significantly among patients who had cavities in the roentgenographic examination, cough at diagnosis and the family history of tuberculosis. Addressing these clinical characteristics will contribute to raising not only the sensitivity of the sputum examination, but also the specificity of the roentgenographic examination in the diagnostic process of tuberculosis.

ANALISIS KINERJA PENYELENGGARAAN MAKANAN DAN TINGKAT KEPUASAN KONSUMEN RESTORAN KHAS PADANG DI BOGOR

Directory of Open Access Journals (Sweden)

Nisa Sinti Atikah

2014-08-01

Full Text Available ABSTRACTThis research aimed to analyze food service performance and level of customer satisfaction in Etnic Padang restaurant in Bogor City. Case study design was applied in this study. Sampling method of purposive sampling was carried out and number of subjects used was 99 people. Based on Importance Performance Analysis, results showed that the most important attribute was cleanliness of dining room and the highest performance levels was safety and hygiene products. Based on Customer Satisfaction Index the satisfaction value gained was 79 (satisfied. Spearman’s correlation showed that there was relationship between income with quality of the product (p<0.05.Keywords: customer satisfaction, ethnic padang restaurant, food serviceABSTRAKPenelitian ini bertujuan untuk menganalisis kinerja penyelenggaraan makanan dan tingkat kepuasan konsumen di Restoran khas Padang, Bogor. Penelitian ini bersifat studi kasus. Metode penarikan subjek dilakukan secara purposive sampling dan jumlah subjek yang digunakan sebanyak 99 orang. Hasil analisis berdasarkan Importance Performance Analysis atribut yang dirasakan paling penting adalah kebersihan ruangan dan tempat makan sedangkan atribut tingkat kinerja yang paling tinggi adalah keamanan dan kebersihan produk. Berdasarkan Customer Satisfaction Index diperoleh nilai kepuasan sebesar 79 (puas. Hasil uji korelasi Spearman menunjukkan adanya hubungan antara pendapatan dengan penilaian atribut mutu produk (p<0.05.Kata kunci: kepuasan konsumen, penyelenggaraan makanan, restoran khas padang

An Integrated Biochemistry Laboratory, Including Molecular Modeling

Science.gov (United States)

Hall, Adele J. Wolfson Mona L.; Branham, Thomas R.

1996-11-01

The dilemma of designing an advanced undergraduate laboratory lies in the desire to teach and reinforce basic principles and techniques while at the same time exposing students to the excitement of research. We report here on a one-semester, project-based biochemistry laboratory that combines the best features of a cookbook approach (high success rate, achievement of defined goals) with those of an investigative, discovery-based approach (student involvement in the experimental design, excitement of real research). Individual modules may be selected and combined to meet the needs of different courses and different institutions. The central theme of this lab is protein purification and design. This laboratory accompanies the first semester of biochemistry (Structure and Function of Macromolecules, a course taken mainly by junior and senior chemistry and biological chemistry majors). The protein chosen as the object of study is the enzyme lysozyme, which is utilized in all projects. It is suitable for a student lab because it is easily and inexpensively obtained from egg white and is extremely stable, and its high isoelectric point (pI = 11) allows for efficient separation from other proteins by ion-exchange chromatography. Furthermore, a literature search conducted by the resourceful student reveals a wealth of information, since lysozyme has been the subject of numerous studies. It was the first enzyme whose structure was determined by crystallography (1). Hendrickson et al. (2) have previously described an intensive one-month laboratory course centered around lysozyme, although their emphasis is on protein stability rather than purification and engineering. Lysozyme continues to be the focus of much exciting new work on protein folding and dynamics, structure and activity (3 - 5). This lab course includes the following features: (i) reinforcement of basic techniques, such as preparation of buffers, simple enzyme kinetics, and absorption spectroscopy; (ii

Evaluation of Bacteriological Stability of Minced Canned Meat ...

African Journals Online (AJOL)

Evaluation of Bacteriological Stability of Minced Canned Meat Stored Under Simulated ... Thermal inactivation of spoilage microorganisms is one of the widely used ... of the aforesaid strains at 104-106 spores/g prior to the heat treatment.

Bacteriological, cytological and histopathological evaluation of the reproductive tract of slaughtered cows

OpenAIRE

Casarin, Julia B.S.; Martini, Ana P.; Trentin, Janislene M.; Fiorenza, Mariani F.; Pessoa, Gilson A.; Barros, Severo S.; Rubin, Mara I.B.

2018-01-01

ABSTRACT: Reproductive diseases, mainly endometritis, are important hurdles in cattle raising, In the current study we evaluated gross, bacteriological, cytological, and histological findings from selected sites of the genital from 23 slaughtered cows and tested whether there is an association between these findings and the probability of reaching a reliable diagnosis. The results from the examinations of macroscopic aspects of uterine secretions, the cytological, bacteriological, and histopa...

LABORATORY DIRECTED RESEARCH AND DEVELOPMENT ANNUAL REPORT TO THE DEPARTMENT OF ENERGY - DECEMBER 2006

Energy Technology Data Exchange (ETDEWEB)

FOX, K.J.

2006-12-31

Brookhaven National Laboratory (BNL) is a multidisciplinary laboratory that carries out basic and applied research in the physical, biomedical, and environmental sciences, and in selected energy technologies. It is managed by Brookhaven Science Associates, LLC, (BSA) under contract with the U. S. Department of Energy (DOE). BNL's total annual budget has averaged about $460 million. There are about 2,500 employees, and another 4,500 guest scientists and students who come each year to use the Laboratory's facilities and work with the staff. The BNL Laboratory Directed Research and Development (LDRD) Program reports its status to the U.S. Department of Energy (DOE) annually in March, as required by DOE Order 413.2B, ''Laboratory Directed Research and Development,'' April 19, 2006, and the Roles, Responsibilities, and Guidelines for Laboratory Directed Research and Development at the Department of Energy National Nuclear Security Administration Laboratories dated June 13, 2006. In accordance this is our Annual Report in which we describe the Purpose, Approach, Technical Progress and Results, and Specific Accomplishments of all LDRD projects that received funding during Fiscal Year 2006.

p-Adic valuation of the Morgan–Voyce sequence and p-regularity

Indian Academy of Sciences (India)

Adic valuation of ... USTHB/Faculty of Mathematics, LATN Laboratory, DG-RSDT, BP 32, El Alia, 16111, Bab Ezzouar, Algiers, Algeria; Ecole nationale Superieure d Informatique (ESI), BP 68M Oued Smar, 16270, El Harrach, Algiers, Algeria ...

Bacteriological Quality and Antibiogram of Isolates from Potable ...

African Journals Online (AJOL)

ADOWIE PERE

Bacteriological Quality and Antibiogram of Isolates from Potable Water Sources in ... microbial analysis revealed a faecal contamination of the groundwater, making it unfit for drinking ... Recent studies shows the proliferation of emerging.

Bacteriological quality of some pharmaceutical products marketed ...

African Journals Online (AJOL)

Bacteriological quality of some pharmaceutical products purchased from open markets, buses and drug stores in Uyo metropolis was studied in order to determine the level of contamination of the drugs. The drug samples examined were Tetracycline capsules, Paracetamol tablets, Ampicillin capsules, Chloroquine tablets, ...

Access to scientific information. A national survey of the Italian Society of Clinical Biochemistry and Laboratory Medicine (SIBioC).

Science.gov (United States)

Lippi, Giuseppe; Ciaccio, Marcello; Giavarina, Davide

2016-09-01

Digital libraries are typically used for retrieving and accessing articles in academic journals and repositories. Previous studies have been published about the performance of various biomedical research platforms, but no information is available about access preferences. A six-question survey was designed by the Italian Society of Clinical Biochemistry and Laboratory Medicine (SIBioC) using the platform Google Drive, and made available for 1 month to the members of the society. The information about the survey was published on the website of SIBioC and also disseminated by two sequential newsletters. Overall, 165 replies were collected throughout the 1-month survey availability. The largest number of replies were provided by laboratory professionals working in the national healthcare system (44.2%), followed by those working in private facilities (13.9%), university professors (12.7%) and specialization training staff (12.7%). The majority of responders published zero to one articles per year (55.2%), followed by two to five articles per year (37.6%), whereas only 7.3% published more than five articles per year. A total of 34.5% of the responders consulted biomedical research platforms on weekly basis, followed by 33.9% who did so on daily basis. PubMed/Medline was the most accessed scientific database, followed by Scopus, ISI Web of Science and Google Scholar. The impact factor was the leading reason when selecting which journal to publish in. The most consulted journals in the field of laboratory medicine were Clinical Chemistry and Laboratory Medicine and Biochimica Clinica. This survey provides useful indications about the personal inclination towards access to scientific information in our country.

Effectiveness of Ivermectin and Albendazole against Haemonchus contortus in Sheep in West Java, Indonesia

Science.gov (United States)

Puspitasari, Silvia; Farajallah, Achmad; Sulistiawati, Erni; Muladno

2016-01-01

Administering a half dose of an anthelmintic is a simple method for detecting resistance in parasites infesting small ruminants. When a single anthelmintic fails in native sheep from Indonesia, a combination of anthelmintics from different chemical classes with different modes of action are administered as an alternative parasite-control strategy. This study compared the anthelmintic efficacy of ivermectin (IVM) and albendazole (ABZ) given either separately as a single dose or half dose or co-administered to sheep naturally infected with Haemonchus contortus. Twelve sheep from Bogor, West Java, Indonesia were divided into the following six treatment groups: half-dose IVM, full-dose IVM, half-dose ABZ, full-dose ABZ, combined IVM + ABZ, and control. The treatment efficacy was determined using the faecal egg count reduction test (FECRT) at day 0 (pre-treatment) and post-treatment at days 7, 14, 21, 28, 35, and 42. The efficacies of half-dose IVM, full-dose IVM, half-dose ABZ, full-dose ABZ, and the combination treatment ranged from −1900% to 100%, 99% to 100%, −167% to 100%, −467% to 89%, and −200% to 100%, respectively. The FECRT for the half-dose IVM, half-dose ABZ, full-dose ABZ showed that H. contortus is resistant to half-dose IVM and ABZ. Full-dose IVM was effective against H. contortus. The combined treatment was more effective against H. contortus than ABZ alone. PMID:27019686

Laboratory testing of extravascular body fluids in Croatia: a survey of the Working group for extravascular body fluids of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Science.gov (United States)

Kopcinovic, Lara Milevoj; Vogrinc, Zeljka; Kocijan, Irena; Culej, Jelena; Aralica, Merica; Jokic, Anja; Antoncic, Dragana; Bozovic, Marija

2016-10-15

We hypothesized that extravascular body fluid (EBF) analysis in Croatia is not harmonized and aimed to investigate preanalytical, analytical and postanalytical procedures used in EBF analysis in order to identify key aspects that should be addressed in future harmonization attempts. An anonymous online survey created to explore laboratory testing of EBF was sent to secondary, tertiary and private health care Medical Biochemistry Laboratories (MBLs) in Croatia. Statements were designed to address preanalytical, analytical and postanalytical procedures of cerebrospinal, pleural, peritoneal (ascites), pericardial, seminal, synovial, amniotic fluid and sweat. Participants were asked to declare the strength of agreement with proposed statements using a Likert scale. Mean scores for corresponding separate statements divided according to health care setting were calculated and compared. The survey response rate was 0.64 (58 / 90). None of the participating private MBLs declared to analyse EBF. We report a mean score of 3.45 obtained for all statements evaluated. Deviations from desirable procedures were demonstrated in all EBF testing phases. Minor differences in procedures used for EBF analysis comparing secondary and tertiary health care MBLs were found. The lowest scores were obtained for statements regarding quality control procedures in EBF analysis, participation in proficiency testing programmes and provision of interpretative comments on EBF's test reports. Although good laboratory EBF practice is present in Croatia, procedures for EBF analysis should be further harmonized to improve the quality of EBF testing and patient safety.

Comprehensive experiment-clinical biochemistry: determination of blood glucose and triglycerides in normal and diabetic rats.

Science.gov (United States)

Jiao, Li; Xiujuan, Shi; Juan, Wang; Song, Jia; Lei, Xu; Guotong, Xu; Lixia, Lu

2015-01-01

For second year medical students, we redesigned an original laboratory experiment and developed a combined research-teaching clinical biochemistry experiment. Using an established diabetic rat model to detect blood glucose and triglycerides, the students participate in the entire experimental process, which is not normally experienced during a standard clinical biochemistry exercise. The students are not only exposed to techniques and equipment but are also inspired to think more about the biochemical mechanisms of diseases. When linked with lecture topics about the metabolism of carbohydrates and lipids, the students obtain a better understanding of the relevance of abnormal metabolism in relation to diseases. Such understanding provides a solid foundation for the medical students' future research and for other clinical applications. © 2014 Biochemistry and Molecular Biology Education.

Infestasi pinjal dan infeksi Dipylidium caninum Linnaeus pada kucing liar di lingkungan kampus Institut Pertanian Bogor, Kecamatan Dramaga

Directory of Open Access Journals (Sweden)

Aulia Syifak Bashofi

2016-02-01

Full Text Available This study was conducted to investigate flea infestation and Dipylidium caninum (Linnaeus 1758 infection on stray cat on Bogor Agricultural University Dramaga. Thirty stray cats were collected from various places around on Bogor Agricultural University Dramaga. The stray cats that showed clinical signs of pruritus and alopecia were collected purposively. The whole body was powdered by gamexan powder, after that the fleas were collected by manual and examined microscopically. The totals of 30 fecal stray cat samples collected and examined toward D. caninum used Mcmaster methode, flotation methode, and saw the existence of proglottid. The result of identification showed that there was found one species of flea, namely Ctenocephalides felis (Bouche 1835, while on faeces was not found D. caninum. Twenty one stray cats were infected by the flea with density average of fleas per cat was 3.8 ± 1.9 individual.

Bacteriological and Physicochemical Qualities of Ebutte River in ...

African Journals Online (AJOL)

Michael Horsfall

Ebutte River water quality studied based on the bacteriological and physicochemical parameters revealed that the human, animal and ... pollution and possible water quality deterioration in ... physicochemical analysis, 1litre new plastic bottles.

Analysis of bias in measurements of potassium, sodium and hemoglobin by an emergency department-based blood gas analyzer relative to hospital laboratory autoanalyzer results.

Directory of Open Access Journals (Sweden)

Jian Bo Zhang

Full Text Available The emergency departments (EDs of Chinese hospitals are gradually being equipped with blood gas machines. These machines, along with the measurement of biochemical markers by the hospital laboratory, facilitate the care of patients with severe conditions who present to the ED. However, discrepancies have been noted between the Arterial Blood Gas (ABG analyzers in the ED and the hospital laboratory autoanalyzer in relation to electrolyte and hemoglobin measurements. The present study was performed to determine whether the ABG and laboratory measurements of potassium, sodium, and hemoglobin levels are equivalent, and whether ABG analyzer results can be used to guide clinical care before the laboratory results become available.Study power analyses revealed that 200 consecutive patients who presented to our ED would allow this prospective single-center cohort study to detect significant differences between ABG- and laboratory-measured potassium, sodium, and hemoglobin levels. Paired arterial and venous blood samples were collected within 30 minutes. Arterial blood samples were measured in the ED by an ABL 90 FLEX blood gas analyzer. The biochemistry and blood cell counts of the venous samples were measured in the hospital laboratory. The potassium, sodium, and hemoglobin concentrations obtained by both methods were compared by using paired Student's t-test, Spearman's correlation, Bland-Altman plots, and Deming regression.The mean ABG and laboratory potassium values were 3.77±0.44 and 4.2±0.55, respectively (P<0.0001. The mean ABG and laboratory sodium values were 137.89±5.44 and 140.93±5.50, respectively (P<0.0001. The mean ABG and laboratory Hemoglobin values were 12.28±2.62 and 12.35±2.60, respectively (P = 0.24.Although there are the statistical difference and acceptable biases between ABG- and laboratory-measured potassium and sodium, the biases do not exceed USCLIA-determined limits. In parallel, there are no statistical differences and

Studying Landslide Displacements in Megamendung (Indonesia Using GPS Survey Method

Directory of Open Access Journals (Sweden)

Hasanuddin Z. Abidin

2004-11-01

Full Text Available Landslide is one of prominent geohazards that frequently affects Indonesia, especially in the rainy season. It destroys not only environment and property, but usually also causes deaths. Landslide monitoring is therefore very crucial and should be continuously done. One of the methods that can have a contribution in studying landslide phenomena is repeated GPS survey method. This paper presents and discusses the operational performances, constraints and results of GPS surveys conducted in a well known landslide prone area in West Java (Indonesia, namely Megamendung, the hilly region close to Bogor. Three GPS surveys involving 8 GPS points have been conducted, namely on April 2002, May 2003 and May 2004, respectively. The estimated landslide displacements in the area are relatively quite large in the level of a few dm to a few m. Displacements up to about 2-3 m were detected in the April 2002 to May 2003 period, and up to about 3-4 dm in the May 2003 to May 2004 period. In both periods, landslides in general show the northwest direction of displacements. Displacements vary both spatially and temporally. This study also suggested that in order to conclude the existence of real and significant displacements of GPS points, the GPS estimated displacements should be subjected to three types of testing namely: the congruency test on spatial displacements, testing on the agreement between the horizontal distance changes with the predicted direction of landslide displacement, and testing on the consistency of displacement directions on two consecutive periods.

Laboratory sample turnaround times: do they cause delays in the ED?

Science.gov (United States)

Gill, Dipender; Galvin, Sean; Ponsford, Mark; Bruce, David; Reicher, John; Preston, Laura; Bernard, Stephani; Lafferty, Jessica; Robertson, Andrew; Rose-Morris, Anna; Stoneham, Simon; Rieu, Romelie; Pooley, Sophie; Weetch, Alison; McCann, Lloyd

2012-02-01

Blood tests are requested for approximately 50% of patients attending the emergency department (ED). The time taken to obtain the results is perceived as a common reason for delay. The objective of this study was therefore to investigate the turnaround time (TAT) for blood results and whether this affects patient length of stay (LOS) and to identify potential areas for improvement. A time-in-motion study was performed at the ED of the John Radcliffe Hospital (JRH), Oxford, UK. The duration of each of the stages leading up to receipt of 101 biochemistry and haematology results was recorded, along with the corresponding patient's LOS. The findings reveal that the mean time for haematology results to become available was 1 hour 6 minutes (95% CI: 29 minutes to 2 hours 13 minutes), while biochemistry samples took 1 hour 42 minutes (95% CI: 1 hour 1 minute to 4 hours 21 minutes), with some positive correlation noted with the patient LOS, but no significant variation between different days or shifts. With the fastest 10% of samples being reported within 35 minutes (haematology) and 1 hour 5 minutes (biochemistry) of request, our study showed that delays can be attributable to laboratory TAT. Given the limited ability to further improve laboratory processes, the solutions to improving TAT need to come from a collaborative and integrated approach that includes strategies before samples reach the laboratory and downstream review of results. © 2010 Blackwell Publishing Ltd.

Photocatalytic generation of hydrogen under visible light on La2CuO4

Indian Academy of Sciences (India)

Laboratory of Storage and Valorization of Renewable Energies, Faculty of Chemistry (USTHB),. B.P. 32, 16111 ... The energy diagram shows the feasibility of La2CuO4 for the H2 evolution under visible light. The best ... An efficient photocata-.

The Biochemistry Tetrahedron and the Development of the Taxonomy of Biochemistry External Representations (TOBER)

Science.gov (United States)

Towns, Marcy H.; Raker, Jeffrey R.; Becker, Nicole; Harle, Marissa; Sutcliffe, Jonathan

2012-01-01

Visual literacy, the ability to interpret and create external representations (ERs), is essential to success in biochemistry. Studies have been conducted that describe students' abilities to use and interpret specific types of ERs. However, a framework for describing ERs derived through a naturalistic inquiry of biochemistry classrooms has not…

Impact of e-resources on learning in biochemistry: first-year medical students' perceptions.

Science.gov (United States)

Varghese, Joe; Faith, Minnie; Jacob, Molly

2012-05-16

E-learning resources (e-resources) have been widely used to facilitate self-directed learning among medical students. The Department of Biochemistry at Christian Medical College (CMC), Vellore, India, has made available e-resources to first-year medical students to supplement conventional lecture-based teaching in the subject. This study was designed to assess students' perceptions of the impact of these e-resources on various aspects of their learning in biochemistry. Sixty first-year medical students were the subjects of this study. At the end of the one-year course in biochemistry, the students were administered a questionnaire that asked them to assess the impact of the e-resources on various aspects of their learning in biochemistry. Ninety-eight percent of students had used the e-resources provided to varying extents. Most of them found the e-resources provided useful and of a high quality. The majority of them used these resources to prepare for periodic formative and final summative assessments in the course. The use of these resources increased steadily as the academic year progressed. Students said that the extent to which they understood the subject (83%) and their ability to answer questions in assessments (86%) had improved as a result of using these resources. They also said that they found biochemistry interesting (73%) and felt motivated to study the subject (59%). We found that first-year medical students extensively used the e-resources in biochemistry that were provided. They perceived that these resources had made a positive impact on various aspects of their learning in biochemistry. We conclude that e-resources are a useful supplement to conventional lecture-based teaching in the medical curriculum.

Successful testing and treating of HIV/AIDS in Indonesia depends on the addiction treatment modality

Directory of Open Access Journals (Sweden)

Iskandar S

2012-12-01

Full Text Available Shelly Iskandar,1–3 Cor AJ de Jong,3 Teddy Hidayat,1 Ike MP Siregar,1 Tri H Achmad,4 Reinout van Crevel,5 Andre Van der Ven51Department of Psychiatry, Padjadjaran University/Hasan Sadikin Hospital, Bandung, Indonesia; 2Health Research Unit, Medical Faculty, Padjadjaran Univervisity, 3Nijmegen Institute for Scientist-Practitioners in Addiction (NISPA, Nijmegen, The Netherlands; 4Department of Biochemistry, Faculty of Medicine, Padjadjaran University/Hasan Sadikin Hospital, Bandung, Indonesia; 5Department of General Internal Medicine and Nijmegen Institute for Inflammation, Infection and Immunity, Radboud University Nijmegen Medical Centre, Nijmegen, The NetherlandsBackground: In many settings, people who inject drugs (PWID have limited access to human immunodeficiency virus (HIV care which is provided in several hospitals and primary health centers in big cities. Substance abuse treatment (SAT can be used as the entry-point to HIV programs. The aim of this study is to describe the characteristics of the PWID who had accessed SAT and determine which SAT modality associates significantly with HIV programs.Methods: PWID were recruited by respondent-driven sampling in an urban setting in Java, Indonesia and interviewed with the Addiction Severity Index (ASI, Blood-Borne Virus Transmission Risk Assessment Questionnaires, and Knowledge Questionnaire on HIV/AIDS. The information regarding the use of substance abuse treatment and HIV program were based on questions in ASI.Results: Seventy-seven percent of 210 PWID had accessed SAT at least once. PWID who had accessed a SAT modality reported more severe drug problems. The most widely used SAT were opioid substitution (57% and traditional/faith-based treatment (56%. Accessing substitution treatment (adjusted odds ratio [OR] = 5.8; 95% confidence interval [CI]: 2.5–13.9 or residential drug-free treatment (adjusted OR = 3.7; 95% CI: 1.4–9.7 was significantly associated with HIV testing, whereas

Role of subtyping in detecting Salmonella cross contamination in the laboratory.

LENUS (Irish Health Repository)

De Lappe, Niall

2009-01-01

BACKGROUND: With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of laboratory cross contamination. METHODS: Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. RESULTS: Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). CONCLUSION: The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.

Nuclear energy research in Indonesia

International Nuclear Information System (INIS)

Supadi, S.; Soentono, S.; Djokolelono, M.

1988-01-01

Indonesia's National Atomic Energy Authority, BATAN (Badan Tenaga Atom Nasional), was founded to implement, regulate and monitor the development and launching of programs for the peaceful uses of nuclear power. These programs constitute part of the efforts made to change to a more industrialized level the largely agricultural society of Indonesia. BATAN elaborated extensive nuclear research and development programs in a variety of fields, such as medicine, the industrial uses of isotopes and radiation, the nuclear fuel cycle, nuclear technology and power generation, and in fundamental research. The Puspiptek Nuclear Research Center has been equipped with a multi-purpose research reactor and will also have a fuel element fabrication plant, a facility for treating radioactive waste, a radiometallurgical laboratory, and laboratories for working with radioisotopes and for radiopharmaceutical research. (orig.) [de

Uncovering protein–protein interactions through a team-based undergraduate biochemistry course

Science.gov (United States)

Cookmeyer, David L.; Winesett, Emily S.; Kokona, Bashkim; Huff, Adam R.; Aliev, Sabina; Bloch, Noah B.; Bulos, Joshua A.; Evans, Irene L.; Fagre, Christian R.; Godbe, Kerilyn N.; Khromava, Maryna; Konstantinovsky, Daniel M.; Lafrance, Alexander E.; Lamacki, Alexandra J.; Parry, Robert C.; Quinn, Jeanne M.; Thurston, Alana M.; Tsai, Kathleen J. S.; Mollo, Aurelio; Cryle, Max J.; Fairman, Robert

2017-01-01

How can we provide fertile ground for students to simultaneously explore a breadth of foundational knowledge, develop cross-disciplinary problem-solving skills, gain resiliency, and learn to work as a member of a team? One way is to integrate original research in the context of an undergraduate biochemistry course. In this Community Page, we discuss the development and execution of an interdisciplinary and cross-departmental undergraduate biochemistry laboratory course. We present a template for how a similar course can be replicated at other institutions and provide pedagogical and research results from a sample module in which we challenged our students to study the binding interface between 2 important biosynthetic proteins. Finally, we address the community and invite others to join us in making a larger impact on undergraduate education and the field of biochemistry by coordinating efforts to integrate research and teaching across campuses. PMID:29091712

Uncovering protein-protein interactions through a team-based undergraduate biochemistry course.

Science.gov (United States)

Cookmeyer, David L; Winesett, Emily S; Kokona, Bashkim; Huff, Adam R; Aliev, Sabina; Bloch, Noah B; Bulos, Joshua A; Evans, Irene L; Fagre, Christian R; Godbe, Kerilyn N; Khromava, Maryna; Konstantinovsky, Daniel M; Lafrance, Alexander E; Lamacki, Alexandra J; Parry, Robert C; Quinn, Jeanne M; Thurston, Alana M; Tsai, Kathleen J S; Mollo, Aurelio; Cryle, Max J; Fairman, Robert; Charkoudian, Louise K

2017-11-01

How can we provide fertile ground for students to simultaneously explore a breadth of foundational knowledge, develop cross-disciplinary problem-solving skills, gain resiliency, and learn to work as a member of a team? One way is to integrate original research in the context of an undergraduate biochemistry course. In this Community Page, we discuss the development and execution of an interdisciplinary and cross-departmental undergraduate biochemistry laboratory course. We present a template for how a similar course can be replicated at other institutions and provide pedagogical and research results from a sample module in which we challenged our students to study the binding interface between 2 important biosynthetic proteins. Finally, we address the community and invite others to join us in making a larger impact on undergraduate education and the field of biochemistry by coordinating efforts to integrate research and teaching across campuses.

Uncovering protein-protein interactions through a team-based undergraduate biochemistry course.

Directory of Open Access Journals (Sweden)

David L Cookmeyer

2017-11-01

Full Text Available How can we provide fertile ground for students to simultaneously explore a breadth of foundational knowledge, develop cross-disciplinary problem-solving skills, gain resiliency, and learn to work as a member of a team? One way is to integrate original research in the context of an undergraduate biochemistry course. In this Community Page, we discuss the development and execution of an interdisciplinary and cross-departmental undergraduate biochemistry laboratory course. We present a template for how a similar course can be replicated at other institutions and provide pedagogical and research results from a sample module in which we challenged our students to study the binding interface between 2 important biosynthetic proteins. Finally, we address the community and invite others to join us in making a larger impact on undergraduate education and the field of biochemistry by coordinating efforts to integrate research and teaching across campuses.

Pengaruh Entrepreneurial Marketing Dan Kebijakan Pemerintah Terhadap Daya Saing Industri Alas Kaki Di Bogor

OpenAIRE

Septiani, Stevia; Sarma, Ma'mun; Limbong, Wilson H

2013-01-01

Entrepreneurial marketing is the suitable approach in terms of resource constraints and problems that exist in small and medium enterprises (SMEs). Footwear industry is a sector of Small and Medium Enterprises (SMEs) that is supported by the government of Bogor. Problems of development of footwear industry are divided into two main factors: the competence of SMEs and local government policy. Based on these problems, this study aims to (1) Identify the characteristics of entrepreneurs, busines...

U.S. Department of Energy, Sandia National Laboratories: Printing Case Study

Science.gov (United States)

The U.S. Department of Energy, Sandia National Laboratories (SNL), New Mexico quantified the costs associated with individual desktop printing devices, for comparison with costs associated with using networked copiers as printers

Error tracking in a clinical biochemistry laboratory

DEFF Research Database (Denmark)

Szecsi, Pal Bela; Ødum, Lars

2009-01-01

BACKGROUND: We report our results for the systematic recording of all errors in a standard clinical laboratory over a 1-year period. METHODS: Recording was performed using a commercial database program. All individuals in the laboratory were allowed to report errors. The testing processes were cl...

Bacteriological Quality and antimicrobial susceptibility of some ...

African Journals Online (AJOL)

In general, the well water samples analyzed in this study were found in unacceptable condition in terms of bacteriological quality. Moreover, the identification of Escherichia coli in some of the wells indicates the safety problem of the water for human consumption. Therefore, it calls for proper disinfection and monitoring of the ...

National Academy of Clinical Biochemistry laboratory medicine practice guidelines for use of tumor markers in testicular, prostate, colorectal, breast, and ovarian cancers

DEFF Research Database (Denmark)

Sturgeon, Catharine M.; Duffy, Michael J.; Stenman, Ulf-Håkan

2008-01-01

BACKGROUND: Updated National Academy of Clinical Biochemistry (NACB) Laboratory Medicine Practice Guidelines for the use of tumor markers in the clinic have been developed. METHODS: Published reports relevant to use of tumor markers for 5 cancer sites--testicular, prostate, colorectal, breast...... for differential diagnosis of nonseminomatous and seminomatous germ cell tumors. Prostate-specific antigen (PSA) is not recommended for prostate cancer screening, but may be used for detecting disease recurrence and monitoring therapy. Free PSA measurement data are useful for distinguishing malignant from benign...... prostatic disease when total PSA is cancer, carcinoembryonic antigen is recommended (with some caveats) for prognosis determination, postoperative surveillance, and therapy monitoring in advanced disease. Fecal occult blood testing may be used for screening asymptomatic adults 50...

Pure water syndrome: Bacteriological quality of Sachet- packed ...

African Journals Online (AJOL)

Pure water syndrome: Bacteriological quality of Sachet- packed drinking water ... of fecal contamination and inadequate water treatment or no treatment at all. ... of hygiene criteria were present in the range of 98 and 106 cfu/100ml of water ...

Onsite assessments for the Department of Energy Laboratory Accreditation Program

International Nuclear Information System (INIS)

McMahan, K.L.

1992-01-01

For Department of Energy (DOE) facilities, compliance with DOE Order 5480.11 became a requirement in January 1989. One of the requirements of this Order is that personal external dosimetry programs be accredited under the Department of Energy's Laboratory Accreditation Program (DOELAP) in Personnel Dosimetry. The accreditation process, from the facility's perspective, is two-fold: dosimeters must meet performance criteria in radiation categories appropriate for each facility, and personnel administering and carrying out the program must demonstrate good operating practices. The DOELAP onsite assessment is designed to provide an independent evaluation of the latter

Study of the bacteriological and physicochemical indicators of ...

African Journals Online (AJOL)

This study investigates the pollution level of surface waters in Zaria, Nigeria. The bacteriological and physicochemical analyses performed were in accordance with standard procedures. Out of 228 samples from different sites, 128 (56.1%) had counts higher than the standards. Samaru stream was the most polluted.

Site-Directed Mutagenesis Study of an Antibiotic-Sensing Noncoding RNA Integrated into a One-Semester Project-Based Biochemistry Lab Course

Science.gov (United States)

Gerczei, Timea

2017-01-01

A laboratory sequence is described that is suitable for upper-level biochemistry or molecular biology laboratories that combines project-based and traditional laboratory experiments. In the project-based sequence, the individual laboratory experiments are thematically linked and aim to show how a bacterial antibiotic sensing noncoding RNA (the…

The PROSEA project (Plant Resources of Southeast Asia)

NARCIS (Netherlands)

Jansen, P.C.M.

1991-01-01

PROSEA publishes its own newsletter which originally was published in Wageningen, the Netherlands, but from April 1991 is edited by J. Kartasubrata and N. Wulijarni-Soetjipto, Herbarium Bogoriense, POB 234, Bogor 16122, Indonesia. From December 10-12, 1990, the Multilateral Steering Committee held

Laboratory methods for diagnosis and detection of drug resistant ...

African Journals Online (AJOL)

Data source: Published series of peer reviewed journals and manuals written on laboratory methods that are currently used for diagnosis and detection of drug resistance of Mycobacterium tuberculosis complex were reviewed using the index medicus, pubmed and medline search. Conventional bacteriological microscopy ...

Pre-Analytical Components of Risk in Four Branches of Clinical Laboratory in Romania--Prospective Study.

Science.gov (United States)

David, Remona E; Dobreanu, Minodora

2016-01-01

Development of quality measurement principles is a strategic point for each clinical laboratory. Preexamination process is the most critical and the most difficult to be managed. The aim of this study is to identify, quantify, and monitor the nonconformities of the pre-analytical process using quality indicators that can affect the patient's health safety in four different locations of a Romanian private clinical laboratory. The study group consisted of all the analysis requests received by the departments of biochemistry, hematology, and coagulation from January through March 2015. In order to collect the pre-analytical nonconformities, we created a "Risk Budget", using the entries from the "Evidence notebook--non-conform samples" from the above mentioned departments. The laboratory established the quality indicators by means of the risk management technique in order to identify and control the sources of errors, FMEA (Failure Modes and Effects Analyses), which had been implemented and monitored for its purposes and special needs. For the assessment of the control level over the processes, the results were transformed on the Six Sigma scale, using the Westgard calculation method and being obtained in this way the frequency with which an error may occur. (https://www.westgard. com/six-sigma-calculators.htm). The obtained results prove that the quantification and monitoring of the indicators can be a control instrument for the pre-analytic activities. The calculation of the Six Sigma value adds extra information to the study because it allows the detection of the processes which need improvement (Sigma value higher than 4 represents a well controlled process). The highest rates were observed for the hemolyzed and the lipemic samples, in the department of biochemistry and hemolyzed, insufficient sample volume, or clotted samples for the department of hematology and coagulation. Significant statistical differences between laboratories participating in the study have

Virtual Laboratories in Chemistry, Biochemistry, & Molecular Biology

DEFF Research Database (Denmark)

May, Michael; Achiam, Marianne

2013-01-01

Report (state-of-the-art review) from a research and development project on virtual laboratories supported by Markedmodningsfonden (tidl. "Fornyelsesfonden")(2012-2014). http://markedsmodningsfonden.dk/projekt/0/34/495.......Report (state-of-the-art review) from a research and development project on virtual laboratories supported by Markedmodningsfonden (tidl. "Fornyelsesfonden")(2012-2014). http://markedsmodningsfonden.dk/projekt/0/34/495....

Bacteriology of aspiration pneumonia in patients with acute coma.

Science.gov (United States)

Lauterbach, Enise; Voss, Frederik; Gerigk, Roland; Lauterbach, Michael

2014-12-01

Loss of protective airway reflexes in patients with acute coma puts these patients at risk of aspiration pneumonia complicating the course of the primary disease. Available data vary considerably with regard to bacteriology, role of anaerobic bacteria, and antibiotic treatment. Our objective was to research the bacteriology of aspiration pneumonia in acute coma patients who were not pre-treated with antibiotics or hospitalized within 30 days prior to the event. We prospectively analyzed 127 patient records from adult patients admitted, intubated and ventilated to a tertiary medical intensive care unit with acute coma. Bacteriology and antibiotic resistance testing from tracheal aspirate sampled within 24 h after admission, blood cultures, ICU scores (APACHE II, SOFA), hematology, and clinical chemistry were assessed. Patients were followed up until death or hospital discharge. The majority of patients with acute coma suffered from acute cardiovascular disorders, predominantly myocardial infarction, followed by poisonings, and coma of unknown cause. In a majority of our patients, microaspiration resulted in overt infection. Most frequently S. aureus, H. influenzae, and S. pneumoniae were isolated. Anaerobic bacteria (Bacteroides spec., Fusobacteria, Prevotella spec.) were isolated from tracheal aspirate in a minority of patients, and predominantly as part of a mixed infection. Antibiotic monotherapy with a 2nd generation cephalosporin, or a 3rd generation gyrase inhibitor, was most effective in our patients regardless of the presence of anaerobic bacteria.

STRENGTHENING BIOTECHNOLOGY RESEARCH IN INDONESIA

Directory of Open Access Journals (Sweden)

S. Sastrapradja

2012-09-01

Full Text Available The wave of biotechnology promises has struck not only the developed countries but the developing countries as well. The scientific community in Indonesia is aware of the opportunities and is eager to take an active part in this particular endeavour. Meanwhile resources are required to welcoming the biotech­nology era. The need of trained manpower, appropriate infrastructure and equipment, operational and maintenance costs requires serious consideration if a unit or a laboratory is expected to be functional in biotechnology. There is a good opportunity of applying biotechnology in the field of agriculture and industry considering the availability of biological resources in Indonesia. This paper outlines what have been done so far, the difficulties encountered and the efforts made to strengthening biotechnology research in Indonesia.

Impact of e-resources on learning in biochemistry: first-year medical students’ perceptions

Science.gov (United States)

2012-01-01

Background E-learning resources (e-resources) have been widely used to facilitate self-directed learning among medical students. The Department of Biochemistry at Christian Medical College (CMC), Vellore, India, has made available e-resources to first-year medical students to supplement conventional lecture-based teaching in the subject. This study was designed to assess students’ perceptions of the impact of these e-resources on various aspects of their learning in biochemistry. Methods Sixty first-year medical students were the subjects of this study. At the end of the one-year course in biochemistry, the students were administered a questionnaire that asked them to assess the impact of the e-resources on various aspects of their learning in biochemistry. Results Ninety-eight percent of students had used the e-resources provided to varying extents. Most of them found the e-resources provided useful and of a high quality. The majority of them used these resources to prepare for periodic formative and final summative assessments in the course. The use of these resources increased steadily as the academic year progressed. Students said that the extent to which they understood the subject (83%) and their ability to answer questions in assessments (86%) had improved as a result of using these resources. They also said that they found biochemistry interesting (73%) and felt motivated to study the subject (59%). Conclusions We found that first-year medical students extensively used the e-resources in biochemistry that were provided. They perceived that these resources had made a positive impact on various aspects of their learning in biochemistry. We conclude that e-resources are a useful supplement to conventional lecture-based teaching in the medical curriculum. PMID:22510159

Virtual Biochemistry – pH effect on enzyme activity

Directory of Open Access Journals (Sweden)

D.N. Heidrich

2011-04-01

Full Text Available Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed  in vitro. Taking as example salivary amylase and the consumption of starch (substrate by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme  activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment.

American journal of biochemistry and biotechnology

National Research Council Canada - National Science Library

2005-01-01

.... Areas covered include: general biochemistry, patho-biochemistry, evolutionary biology, structural biology, molecular and cellular biology, molecular medicine, cancer research, virology, immunology, plant molecular biology...

Use of Mushroom Tyrosinase to Introduce Michaelis-Menten Enzyme Kinetics to Biochemistry Students

Science.gov (United States)

Flurkey, William H.; Inlow, Jennifer K.

2017-01-01

An inexpensive enzyme kinetics laboratory exercise for undergraduate biochemistry students is described utilizing tyrosinase from white button mushrooms. The exercise can be completed in one or two three-hour lab sessions. The optimal amounts of enzyme, substrate (catechol), and inhibitor (kojic acid) are first determined, and then kinetic data is…

Ragam Cendawan Mikoriza Arbuskula pada Areca di Kebun Raya Bogor

Directory of Open Access Journals (Sweden)

Ratu Aqila Fahriny

2016-01-01

Full Text Available Areca known as the pinang tree and have benefit as a powerful medicine on men. Arbuscular mycorrhizal fungi (AMF are symbiotic on almost every root plants, but the diversity of AMF on rizosfer Areca is unknown. Therefore research on variety and colonies AMF on rizosfer Areca is needed. Soil samples of Areca were isolated and processed by wet sieving and decauting method. Root samples of Areca were stained by using trypan blue in lactoglycerol. There were 3 genera of AMF collected from Bogor Botanical Garden (4 species Acaulospora, 3 species Glomus, and 2 species Scutellospora which is associated with 3 species of Areca. Percentage colonies of AMF rizosfer on roots Areca was counted. Areca has an intermediate type of arbuscular.

Organ bacteriological dynamics in Heterobranchus bidorsalis ...

African Journals Online (AJOL)

The effect of dietary intake of Lactobacillus fermentum, Saccharomyces cerevisiae and their combination on selected tissue bacteriology of Heterobranchus bidorsalis juveniles was investigated. Ten experimental diets at 41.0% crude protein were prepared to include Lf1 (basal diet + 10¹cfu/mL Lactobacillus fermentum), Lf2 ...

Ayah Permisif Meningkatkan Risiko Anak Untuk Merokok

OpenAIRE

Fadilahtul Husna; Dwi Hastuti

2015-01-01

Today, the problems of adolescent especially in smoking preference in Indonesia is very alarming. This study was conducted to analyze the influence of fathering on smoking behavior of junior high school students in rural area of Bogor. The design of this research was cross sectional study and used survey method. This study involved 60 students of seventh and eighth grades in two junior high schools in the Pamijahan Sub District, Bogor District, West Java Province that were selected by proport...

Bacteriological and Physicochemical Quality of Drinking Water and ...

African Journals Online (AJOL)

BACKGROUND: Lack of safe drinking water, basic sanitation, and hygienic practices are associated with high morbidity and mortality from excreta related diseases. The aims of this study were to determine the bacteriological and physico-chemical quality of drinking water and investigate the hygiene and sanitation practices ...

A Survey of Final-year Undergraduate Laboratory Projects in Biochemistry and Related Degrees in Great Britain.

Science.gov (United States)

Austin, Caroline A.

1997-01-01

Analyzes undergraduate research projects in biochemistry and related subjects at British universities. Discusses the trend toward students doing less research as part of their undergraduate study. Reasons cited for this trend include increased student numbers and costs. (DDR)

APLIKASI NPV AT RISK DALAM ANALISIS KELAYAKAN FINANSIAL BUDIDAYA JAMUR TIRAM PUTIH DI KABUPATEN BOGOR JAWA BARAT

Directory of Open Access Journals (Sweden)

Tintin Sarianti

2011-08-01

Full Text Available Normal 0 false false false MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} The purpose of this study where to analyze white oyster mushroom farming activity in Sub-Province of Bogor, to analyze financial feasibility of  white oyster mushroom farming activity for high and low land in Sub-Province of Bogor by NPV at Risk Method and to analyze the switching value for the changes of cost and benefit in  white oyster mushroom farming activity.  The data were collected from 6 farmer living in high and low land in Sub-Province of Bogor. The NPV at risk model and switching value analysis were used to analyze tha data.  The result of the study showed that white oyster mushroom farming activity for high and low land in Sub-Province of Bogor were feasible by financial that considered by investors in selecting the location to act the white oyster mushroom farming.  The risks like price risk, production risk in that activity have accomodated in to cash flow.  Accomodation for price risk was used the inflation rate and for production risk was used estimation for failure of crop in white oyster mushroom farming activity according the farmers in location.  Switching value was used for to analyze maximum changes in cost and benefit which were developing the cash flow.  The maximum changes describes that the changes admit of tolerated.  The result for switching value analysis showed that the decreasing of production of white oyster mushroom (fresh and baglog more sensitive than the others (decreasing of fresh white oyster mushroom price and increasing of varible

Safety leadership in the teaching laboratories of electrical and electronic engineering departments at Taiwanese Universities.

Science.gov (United States)

Wu, Tsung-Chih

2008-01-01

Safety has always been one of the principal goals in teaching laboratories. Laboratories cannot serve their educational purpose when accidents occur. The leadership of department heads has a major impact on laboratory safety, so this study discusses the factors affecting safety leadership in teaching laboratories. This study uses a mail survey to explore the perceived safety leadership in electrical and electronic engineering departments at Taiwanese universities. An exploratory factor analysis shows that there are three main components of safety leadership, as measured on a safety leadership scale: safety controlling, safety coaching, and safety caring. The descriptive statistics also reveals that among faculty, the perception of department heads' safety leadership is in general positive. A two-way MANOVA shows that there are interaction effects on safety leadership between university size and instructor age; there are also interaction effects between presence of a safety committee and faculty gender and faculty age. It is therefore necessary to assess organizational factors when determining whether individual factors are the cause of differing perceptions among faculty members. The author also presents advice on improving safety leadership for department heads at small universities and at universities without safety committees.

Studies of biochemistry and clinical biochemistry. Studies at sample medical schools in 13 EU countries regarding biochemistry and clinical biochemistry teaching.

Science.gov (United States)

Stern, Petr; Sebesta, Ivan; Trnkova, Bohuslava; Zima, Tomas

2008-07-01

The study summarizes the results obtained during personal visits to 53 medical schools in the 13 original EU countries during 2004--2006. Data from the Czech Republic is shown for comparison. The possibilities of acquiring information from the websites of the medical schools in the local language and English are assessed. The admission process to medical schools and the organization of studies of medicine, dentistry, and non-medical healthcare fields are briefly characterized. Significant attention is paid to the forms of education in biochemistry and clinical (bio)chemistry in the medical study field. The position of these subjects in the studies of dentistry and non-medical healthcare fields is also noted. In addition, the course of subject exams is described. The methods of funding and postgraduate studies at the medical schools are also briefly addressed.

ISOLATION AND IDENTIFICATION OF BRUCELLA SUIS IN PIGS AS ZOONOTIC DISEASE IN ENDEMIC AREAS OF EAST JAVA, INDONESIA.

Science.gov (United States)

S, Emy Koestanti; Misaco, Wiwik; Chusniati, Sri; Maslachah, Lilik

2018-01-01

Brucellosis in pigs at East Java Indonesia has not only cause great economic losses due to a decrease in productivity of livestock but also are zoonotic. Infection on free brucelosis pigs were initially begun with the infected pigs both male and female, or the use of superior male pigs together. The elimination of the disease either on a group or population is considered as the most effective way to prevent the spread of the disease in pigs. Prevention efforts mainly addressed to vaccination, sanitary maintenace and government policy. The purpose of this study was to isolated and identified Brucella suis as the causative agent. The survey area were the pig farm owned by breeder farmers in the area of East Java Indonesia, at Kediri, Malang, Blitar and Probolinggo district. Blood samples obtained were tested with RBT. Pigs are suspected of being infected with Brucella if the RBT was positive that characterized with agglutination in the test results. If RBT was positive, bacteriological examination will be performed, with samples of visceral foetus organ, ie liver, spleen, placenta and amniotic fluid. Isolation and identification of Brucella suis were used Brucella Broth and Brucella Agar, and if the bacteri growthwill be continued with biochemical test ie H2S, urease, citrate, catalase and oxidase test. The positive results of Brucella suis showed positive urease, catalase andoxidase, but negative for citrate and H2S. RBT and bacteriolgical examination showed that 1 sample was positive Brucella suis , and 19 negative. The positive results showed positive urease, catalase and oxidase, but negative for citrate and H2S. Based on RBT test and bacteriological examination, there was 1 positive sample of brucellla suis, that is sample coming from Kediri district.

Comparative study on the level of bacteriological contamination of ...

African Journals Online (AJOL)

Comparative study on the level of bacteriological contamination of automatic teller machines, public toilets and public transport commercial motorcycle crash ... Conclusion: This study has revealed the ability of these public devices to serve as vehicle of transmission of microorganisms with serious health implications.

[Verification of bacteriological safety of PCM 40 air conditioner].

Science.gov (United States)

Dumas, J L; Ducel, G; Rouge, J C

1991-01-01

This study assessed the bacteriological safety of the bedside air conditioner PCM 40 (Howorth Airtech), used for prevention of intraoperative hypothermia, by blowing filtered warm air through a special mattress. The 3 microns bacterial filter of the device released 2,968 +/- 5,618 particles of diameter less than 3 microns per m3 of room air, containing 78,798 +/- 37,243 of such particles per m3. The amount of bacteries in the air pulsed from the mattress was 30 +/- 41 cfu/m3 vs 120 cfu/m3 in the ambient air and in the hot air supply tubing it reached 6 +/- 5 cfu/m3 vs 175 +/- 77 cfu/m3. It is concluded that bacteriological data do not contra-indicate the use of this air conditioner in the operating theater. The only limitations for use are the position (prone or lateral position) and type of surgery (neurosurgery).

Learning Biochemistry by Chocolate

Directory of Open Access Journals (Sweden)

M.C Guedes

2006-07-01

Full Text Available Both sensations and biochemical reactions taken place or promoted during ingestion of chocolate were the motivation for  investigating  the  organic  compounds  present  in  this  source.  Cocoa  and  chocolate  are  composed  by  several substances , among them, aminoacids and alkaloids.The objective of this investigation was to purpose a contextured approach  of  biochemistry  through  the  sensations  and  reactions  involving  aminoacids,  theobromine  and  hormones. Methodology: 1. Theoretical part:  constituted  by theoretical  and tutorial classes  about aminoacids, theobromine and hormones  involved  at  the  metabolism;  2.  Questionary:  ten  questions  based  upon  theoretical  classes,  personal sensations  and  general  aspects  of chocolate;  3.Lecture:  Cientific  articles  searched  in  periodics  by  own  students  as well  as  newspaper  reports;  4.  Experimental:  Laboratory  experiments  including  extraction,  characterization, spectrometric quantification  after  specific reactions  and identification by  Rf  comparison with  standards  on TLC  from cocoa  almonds  and  both  powder  cocoa  and  chocolate.  The  study  was  applied  in  30  students  from  a  chemistry college. Results: The results pointed out to a higher frequency of the students and to a increased interest  from them by   biochemistry  issues  and  cientific  lectures,  as  well  as  a  satisfactory  acquirement  of  theoretical  and  practice knowledge of aminoacids and hormones, spectrometry and chromatography. Conclusion: A contextured approach is quite positive for learning biochemistry to chemists.

Liver function and bacteriology of organs in broiler inoculated with nalidixic acid-resistant Salmonella Typhimurium and treated with organic acids

Directory of Open Access Journals (Sweden)

Tatiane M. Rocha

2013-07-01

Full Text Available AbAns etxrpaecritment was carried out with 630 one-day-old chicks to evaluate the effects of organic acids when birds were experimentally inoculated with Salmonella Typhimurium. Liver damage and the persistence of the bacterium in the organs were evaluated as well. Broilers were distributed in a completely randomised experimental design in a 3×2 factorial arrangement of six treatments with seven replicates of 15 birds each. Birds were inoculated with saline solution or the bacterium via gavage at 1 day of age, or were offered a feed containing or not the organic acid blend for the period of 7 to 14 days of age. A dose of 5.0x102 colony-forming units (CFU/0.5 mL of Salmonella Typhimurium was used for inoculation both via gavage and feed. The parameters evaluated are weight, liver histopathology, liver and serum biochemistry, and bacteriological analyses of the caeca, crop, spleen, and liver and heart pool. At 21 and 28 days of age, the liver of the non-inoculated groups was significantly lighter as compared to the other treatments. Birds fed organic acids presented lower bacterial isolation rates in all organs tested. Birds inoculated in the crop and treated with organic acids presented lower E. coli CFU counts (P<0.05. Birds inoculated with Salmonella presented significant changes (P<0.05 in liver enzymes, as detected by serum biochemistry, and in liver histopathology. It was concluded that organic acids effectively controlled Salmonella Typhimurium and did not cause any liver damage.

Biochemical Neuroscience Laboratory

Data.gov (United States)

Federal Laboratory Consortium — This biochemistry lab is set up for protein analysis using Western blot, enzyme linked immunosorbent assays, immunohistochemistry, and bead-based immunoassays. The...

Assessment of physico-chemical and bacteriological quality of ...

African Journals Online (AJOL)

user

Water quality is a critical factor affecting human health and welfare. This study aimed at examining the physico-chemical and bacteriological quality of drinking water in Adama town. A total of 107 triplicate water samples were examined; 1 from inlet point (raw water), 1 from outlet (the water after treatment, 1 from reservoir ...

Water Quality & Pollutant Source Monitoring: Field and Laboratory Procedures. Training Manual.

Science.gov (United States)

Office of Water Program Operations (EPA), Cincinnati, OH. National Training and Operational Technology Center.

This training manual presents material on techniques and instrumentation used to develop data in field monitoring programs and related laboratory operations concerned with water quality and pollution monitoring. Topics include: collection and handling of samples; bacteriological, biological, and chemical field and laboratory methods; field…

75 Breakthroughs by the U.S. Department of Energy's National Laboratories; Breakthroughs 2017

Energy Technology Data Exchange (ETDEWEB)

None

2017-01-01

Born at a time when the world faced a dire threat, the National Laboratory System protects America through science and technology. For more than 75 years, the Department of Energy’s national laboratories have solved important problems in science, energy and national security. Partnering with industry and academia, the laboratories also drive innovation to advance economic competitiveness and ensure our nation’s future prosperity. Over the years, America's National Laboratories have been changing and improving the lives of millions of people and this expertise continues to keep our nation at the forefront of science and technology in a rapidly changing world. This network of Department of Energy Laboratories has grown into 17 facilities across the country. As this list of breakthroughs attests, Laboratory discoveries have spawned industries, saved lives, generated new products, fired the imagination and helped to reveal the secrets of the universe.

The Beginning Lecture and the Improvement of “Experiments in Innovative Chemistry” as an Entry Subjects at the Department of Biochemistry and Applied Chemistry in National College of Technology

Science.gov (United States)

Tsuda, Yusuke; Nakashima, Hiroyuki; Tsuji, Yutaka; Watanabe, Katsuhiro; Ooka, Hisako

The beginning lecture and the improvement of “Experiments in Innovative Chemistry” as an entry subjects in the Department of Biochemistry and Applied Chemistry at Kurume National College of Technology has been performed for recent three years. Every experiment was selected to sustain the young student's interest. The questionnaires were performed after first two year's programs were finished, and some of projects were improved. This subject has a good reputation for students and teachers, and seems to be very effective for the first year students of national college of technology.

Dose measurements in laboratory of Physics department, University of Khartoum

International Nuclear Information System (INIS)

Hamid, Maria Mohammed

1999-05-01

Personal monitoring in University of Khartoum is being conducted using thermoluminescent dosimetry. The purpose of the study is to measure the dose of radiation in laboratory of Physics in physics department. TL phosphors LiF: Mg, Ti (card) and LiF Mg, Cu, P (GR-200) and mini-rad dosimeter are used to measure the dose in laboratory. The total dose for students form the laboratory bu using card, GR-200 and mini-rad dosimeter was found to be 2.2μ sv/year. 2.5 μ sv/year and 2.6 μ sv respectively, and for the teacher about 4.0 μ sv/year, 5.8 μ sv/year and 13.6 μ sv/year respectively, and for the dose near junk room about 3.9 μ sv/year, 2.9 μ sv/year and 2.8 μ sv/year by using card, GR-200 and mini-rad dosimeter respectively. There is just a background radiation in the main library and the applied nuclear.(Author)

[The experience of implementation of system of quality management in the Department of Laboratory Diagnostic of the N.V. Sklifosofskiy Research Institute of Emergency Care of Moscow Health Department: a lecture].

Science.gov (United States)

Zenina, L P; Godkov, M A

2013-08-01

The article presents the experience of implementation of system of quality management into the practice of multi-field laboratory of emergency medical care hospital. The analysis of laboratory errors is applied and the modes of their prevention are demonstrated. The ratings of department of laboratory diagnostic of the N. V. Sklifosofskiy research institute of emergency care in the program EQAS (USA) Monthly Clinical Chemistry from 2007 are presented. The implementation of the system of quality management of laboratory analysis into department of laboratory diagnostic made it possible to support physicians of clinical departments with reliable information. The confidence of clinicians to received results increased. The effectiveness of laboratory diagnostic increased due to lowering costs of analysis without negative impact to quality of curative process.

Tensions within an industrial research laboratory: the Philips laboratory's x-ray department between the wars

NARCIS (Netherlands)

Boersma, F.K.

2003-01-01

Tensions arose in the X-ray department of the Philips research laboratory during the interwar period, caused by the interplay among technological development, organizational culture, and individual behavior. This article traces the efforts of Philips researchers to find a balance between their

Transit Oriented Development (TOD) index at the current transit nodes in Depok City, Indonesia

Science.gov (United States)

Sulistyaningrum, Subekti; Sumabrata, Jachrizal

2018-03-01

Depok is a rural area which developed by Perum Perumnas in 1976. And growth rapidly since University of Indonesia moved in 1986. Afterward, Depok became a new city at periphery of DKI Jakarta with numerous development and urbanizations. Depok city is facing urban problems including population growth of 9% per year, the increasing regular and irregular settlements and transportation problems such as traffic congestion, the increasing of individual cars and motorcycles and lack of transportation development. Moreover, Jabodetabek Commuterline which is start from Bogor station and transits in 5 (five) stations in Depok city (Universitas Indonesia, Pondok Cina, Depok Baru, Depok and Citayam), become a potential alternative for commuters in Depok to go out of the city. Depok should have sustainable transportation such as Transit Oriented Development (TOD) masterplan in a strategic place of railways area to conduct a sustainable city. This study discovered that TOD planning should be proceded by understanding the existing condition through measurement of TOD level. Therefore, the measurement of TOD level around the existing nodes is inevitable as transit node plays a central role in TOD, so it was defined as 800 meters walking distances. This paper focus on 3 (three) station areas along the way of Jalan Margonda, which has a very significant growth development area in Depok with the indicators are devided into spatial and non-spatial indicators. Geographic Information Systems (ArcGIS) isused to quantify all of the spatial indicators.

Department of Energy Multiprogram Laboratories

International Nuclear Information System (INIS)

1982-09-01

Volume III includes the following appendices: laboratory goals and missions statements; laboratory program mix; class waiver of government rights in inventions arising from the use of DOE facilities by or for third party sponsors; DOE 4300.2: research and development work performed for others; procedure for new work assignments at R and D laboratories; and DOE 5800.1: research and development laboratory technology transfer program

Bacteriological and physico-chemical quality of drinking water ...

African Journals Online (AJOL)

Background: Accesses to safe water is a universal need however, many of the world's population lack access to adequate and safe water. Consumption of water contaminated causes health risk to the public and the situation is serous in rural areas. Objectives: To assess the bacteriological and physico-chemical quality of ...

December 2004 Sumatra, Indonesia Images

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The December 26, 2004 magnitude 9.1 Sumatra, Indonesia earthquake (3.316 N, 95.854 E, depth 30 km) generated a tsunami that was observed worldwide and caused...

Isolation and identification of bacterial pathogen from mastitis milk in Central Java Indonesia

Science.gov (United States)

Harjanti, D. W.; Ciptaningtyas, R.; Wahyono, F.; Setiatin, ET

2018-01-01

Mastitis is a multi-etiologic disease of the mammary gland characterized mainly by reduction in milk production and milk quality due to intramammary infection by pathogenic bacteria. Nearly 83% of lactating dairy cows in Indonesia are infected with mastitis in various inflammation degrees. This study was conducted to isolate and identify the pathogen in milk collected from mastitis-infected dairy cows. The study was carried out in ten smallholder dairy farms in Central Java Indonesia based on animal examination, California mastitis test, isolation bacterial pathogens, Gram staining, Catalase and Coagulase test, and identification of bacteria species using Vitek. Bacteriological examination of milk samples revealed 15 isolates where Streptococcus was predominant species (73.3%) and the coagulase negative Staphylococcus species was identified at the least bacteria (26.7%). The Streptococcus bacteria found were Streptococcus uberis (2 isolates), Streptococcus sanguinis(6 isolates), Streptococcus dysgalactiaessp dysgalactiae(1 isolate) , Streptococcus mitis (1 isolate) and Streptococcus agalactiae (1 isolate). The Staphylococcus isolates comprising of Staphylococcus simulans (1 isolate) and Staphylococcus chromogens (3 isolates). Contamination of raw milkwith pathogenic bacteria can cause outbreaks of human disease (milk borne disease). Thus, proper milk processing method that couldinhibit the growth or kill these pathogenic bacteria is important to ensure the safety of milk and milk products.

[Current status of bacteriological studies at prefectural and municipal public health institutes in Japan].

Science.gov (United States)

Shinomiya, Hiroto; Seto, Kazuko; Kawase, Jun; Arikawa, Kentaro; Funatogawa, Keiji; Suzuki, Masahiro; Kubota, Hiroaki; Shirabe, Komei

2015-01-01

Prefectural and municipal public health institutes are located in prefectures and ordinance-designated cities in Japan, and play a vital role in the regional surveillance of infectious diseases and foodborne illnesses. These institutes, in close cooperation with national institutes such as the National Institute of Infectious Diseases and the National Institute of Health Sciences, construct the national surveillance network for infectious diseases and their causative agents. Bacteriological examinations and studies on a variety of infectious diseases and foodborne illnesses are core activities of prefectural and municipal public health institutes, through which novel and important bacteriological findings have been acquired. In this article, we report the latest findings regarding bacteriological examinations/studies and interesting cases at these institutes, especially concerning foodborne illnesses, tuberculosis, and antimicrobial resistances.

Physicochemical and bacteriological quality of bottled drinking water in three sites of Amhara Regional State, Ethiopia.

Science.gov (United States)

Biadglegne, Fantahun; Tessema, Belay; Kibret, Mulugeta; Abera, Bayeh; Huruy, Kahsay; Anagaw, Belay; Mulu, Andargachew

2009-10-01

The consumption of bottled drinking water is becoming increasing in Ethiopia. As a result there has been a growing concern about the chemical, physical and bacteriological quality of this product. Studies on the chemical, physical and bacteriological quality of bottled water is quite scarce in Ethiopia. This study was therefore aimed to assess the physicochemical and bacteriological qualities of three factories of bottled drinking water products produced in Amhara region. A Laboratory based comparative study was conducted to evaluate the physicochemical and bacteriological quality of three factories of bottled drinking water produced in Amhara region. Analysis on the quality of bottled drinking water from the sources, wholesalers and retailers were made with World Health Organization and Quality and Standards Authority of Ethiopia recommendations. Triplicate samples from three types of bottled drinking water were randomly collected and analyzed from June, 2006 to December, 2006. A total of 108 commercial bottled drinking water samples were analyzed. The result showed that except pH of factory A all the physicochemical parameters analyzed were with in the recommended limits. The pH value of factory A tested from sources is 5.3 and from wholesalers and retailers is 5.5 and 5.3, respectively, which is below the normal value set by World Health Organization (6.5-8.0) and Quality and Standards Authority of Ethiopia (6.0-8.5). Our analyses also demonstrated that 2 (16.7%) of the samples tested from sources and 1 (8.3%) from wholesalers of factory B were contaminated with total coliforms, where as 2 (16.7%) samples from retailers were also contaminated with total coliforms. On the other hand, 1 (8.3%) of the samples tested from wholesalers and 2 (16.7%) of the samples tested from retailers of factory A were also contaminated with total coliforms. Total coliforms were not detected from all samples of factory C, fecal coliforms were not also isolated from all samples

FEATURES OF SCIENTIFIC INVESTIGATIONS CONDUCTED IN THE LABORATORIES OF THE DEPARTMENT OF WATER SUPPLY OF MGSU

OpenAIRE

Nikitina Irina Nikolaevna; Eremeev Aleksandr Vladimirovich

2016-01-01

The article focuses on the work of the laboratories of the Department of Water Supply of MGSU. The laboratory of pipe-lines, pumping equipment and sanitary equipment operates in MGSU affiliated to the department of water supply. A hydraulic stand for testing and defining the the hydraulic characteristics of pressure and free-flow pipelines of water supply and sewerage systems is installed there. There are also stands for investigating the sanitary equipment of the buildings, the fire and hot ...