An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

Energy Technology Data Exchange (ETDEWEB)

Rothe, Sebastian

2012-09-24

This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

Determination of the electron affinity of astatine and polonium by laser photodetachment

CERN Multimedia

We propose to conduct the first electron affinity (EA) measurements of the two elements astatine (At) and polonium (Po). Collinear photo-detachment spectroscopy will allow us to measure these quantities with an uncertainty limited only by the spectral line width of the laser. We plan to use negative ion beams of the two radioactive elements At and Po, which are only accessible on-line and at ISOLDE. The feasibility of our proposed method and the functionality of the experimental setup have been demonstrated at ISOLDE in off-line tests by the clear observation of the photo-detachment threshold for stable iodine. This proposal is based on our Letter of Intent I-148.

Thermogravimetric determination of the enthalpy of astatine and radon adsorption on palladium surfaces

International Nuclear Information System (INIS)

Eichler, B.; Son Chun, K.

1985-01-01

In order to investigate the adsorption of astatine and radon on a palladium surface some on- and off-line thermochromatographic experiments were carried out with 210 At and 220 Rn tracers. The partial molar adsorption enthalpy for zero covering was found to be ΔH/sub a//sup 0, loc./(At) = -(15S +- 10) kJ mole -1 and ΔH/sub a//sup 0, mob./(Rn) = -(37 +- 4) kJ mole -1 . The results are compared with theoretical and experimental values for other elements of the sixth period. The adsorption behaviour of At is in conformity with that of the p-metals on a palladium surface. (author)

Estimation of the chemical form and the boiling point of elementary astatine by radiogas-chromatography

International Nuclear Information System (INIS)

Otozai, K.; Takahashi, N.

1982-01-01

After astatine (0) was mixed with 131 I 2 containing carrier I 2 , the sample was analyzed by means of radiogaschromatography and the peaks due to I 2 , AtI and At 2 were observed. Further, the boiling points were estimated from the retention volume in terms of the semi-empirical theory on gas chromatography. The boiling points of I 2 , AtI and At 2 were 457 +- 2,486 +- 2 and 503 +- 3K, respectively. The boiling point of At 2 obtained in the present work is far smaller than that expected by the extrapolation of lighter halogens. (orig.)

49 CFR 571.212 - Standard No. 212; Windshield mounting.

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2010-10-01

... 49 Transportation 6 2010-10-01 2010-10-01 false Standard No. 212; Windshield mounting. 571.212... Motor Vehicle Safety Standards § 571.212 Standard No. 212; Windshield mounting. S1. Scope. This standard..., under the conditions of S6, the windshield mounting of the vehicle shall retain not less than the...

Laser photodetachment of radioactive ions: towards the determination of the electronegativity of astatine

CERN Multimedia

Rothe, Sebastian; Welander, Jakob Emanuel; Chrysalidis, Katerina; Day Goodacre, Thomas; Fedosseev, Valentine; Fiotakis, Spyridon; Forstner, Oliver; Heinke, Reinhard Matthias; Johnston, Karl; Kron, Tobias; Koester, Ulli; Liu, Yuan; Marsh, Bruce; Ringvall Moberg, Annie; Rossel, Ralf Erik; Seiffert, Christoph; Studer, Dominik; Wendt, Klaus; Hanstorp, Dag

2017-01-01

Negatively charged ions are mainly stabilized through the electron correlation effect. A measure of the stability of a negative ion is the electron affinity, which the energy gain by attaching an electron to a neutral atom. This fundamental quantity is, due to the almost general lack of bound excited states, the only atomic property that can be determined with high accuracy for negative ions. We will present the results of the first laser photodetachment studies of radioactive negative ions at CERN-ISOLDE. The photodetachment threshold for the radiogenic iodine isotope 128I was measured successfully, demonstrating the performance of the upgraded GANDALPH experimental beam line. The first detection of photo-detached astatine atoms marks a milestone towards the determination of the EA of this radioactive element.

Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

CERN Document Server

Andreyev, Andrei

2013-01-01

Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

Complexation study on no-carrier-added astatine with insulin: A candidate radiopharmaceutical

Energy Technology Data Exchange (ETDEWEB)

Lahiri, Susanta [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India)], E-mail: susanta.lahiri@saha.ac.in; Roy, Kamalika [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India); Sen, Souvik [Berhampur Sadar Hospital, Berhampur, Murshidabad 742 101 (India)

2008-12-15

No-carrier-added astatine radionuclides produced in the {sup 7}Li-irradiated lead matrix were separated from bulk lead nitrate target by complexing At with insulin, followed by dialysis. The method offers simultaneous separation of At from lead as well as its complexation with insulin. The At-insulin complex might be a potential radiopharmaceutical in the treatment of hepatocellular carcinoma. The stability of At-insulin complex was checked by dialysis against deionized water and Ringer lactate (RL) solution. It has been found that the half-life of At-insulin complex is about {approx}12 h, when dialyzed against deionized water and is only 6 h, when dialyzed against RL solution having the same composition as blood serum. The 6 h half-life of this Insulin-At complex is perfect for killing cancer cells from external cell surfaces as the half-life of internalization of insulin molecule inside the cell is 7-12 h.

Complexation study on no-carrier-added astatine with insulin: A candidate radiopharmaceutical

International Nuclear Information System (INIS)

Lahiri, Susanta; Roy, Kamalika; Sen, Souvik

2008-01-01

No-carrier-added astatine radionuclides produced in the 7 Li-irradiated lead matrix were separated from bulk lead nitrate target by complexing At with insulin, followed by dialysis. The method offers simultaneous separation of At from lead as well as its complexation with insulin. The At-insulin complex might be a potential radiopharmaceutical in the treatment of hepatocellular carcinoma. The stability of At-insulin complex was checked by dialysis against deionized water and Ringer lactate (RL) solution. It has been found that the half-life of At-insulin complex is about ∼12 h, when dialyzed against deionized water and is only 6 h, when dialyzed against RL solution having the same composition as blood serum. The 6 h half-life of this Insulin-At complex is perfect for killing cancer cells from external cell surfaces as the half-life of internalization of insulin molecule inside the cell is 7-12 h

The chemical fate of 212Bi-DOTA formed by β- decay of 212Pb(DOTA)2-

International Nuclear Information System (INIS)

Mirzadeh, S.; Kumar, K.; Gansow, O.A.

1993-01-01

The increasing use of inert metal complexes in radioimmunotherapy prompted us to explore the potential use of 212 Pb chelates. Herein, we report a study of the chemical fate of the 212 Bi-DOTA complex formed by β - decay of 212 Pb(DOTA) 2- (H 4 DOTA = 1,4,7,10-tetraazacyclododecanetetraacetic acid). To assure that both parent and daughter complexes were thermally stable, kinetic studies were performed with 203 Pb(II) and 206 Bi(III) which showed that both lead and bismuth complexes with DOTA undergo chemical exchange only very slowly in aqueous solution at pH 4-10. To investigate whether the complex ion which results from decay of 212 Pb(DOTA) 2- was intact and also stable, solutions initially containing only this ion were analyzed for amounts of DOTA-complexed and uncomplexed 212 Bi after attaining transient equilibrium with 212 Bi. The fraction of 212 Bi radioactivity not complexed to DOTA, vide infra, was found to be 36±2%. This value represents the fraction of breakup of 212 Bi(DOTA) - formed from β - decay of the parent complex. By considering the various extranuclear processes responsible for kinetic and electronic excitation of the 212 Bi daughter, break-up of the 212 Bi-DOTA complex is ascribed to the internal conversion of γ-rays emitted by the excited 212 Bi nuclide. (orig.)

Final Report for research grant "Development of Methods for High Specific Activity Labeling of Biomolecules Using Astatine-211 in Different Oxidation States"

Energy Technology Data Exchange (ETDEWEB)

Wilbur, D. Scott [Univ. of Washington, Seattle, WA (United States)

2011-12-14

The overall objective of this research effort was to develop methods for labeling biomolecules with higher oxidation state species of At-211. This was to be done in an effort to develop reagents that had higher in vivo stability than the present carbon-bonded At-211-labeled compounds. We were unsuccessful in that effort, as none of the approaches studied provided reagents that were stable to in vivo deastatination. However, we gained a lot of information about At-211 in higher oxidation states. The studies proved to be very difficult as small changes in pH and other conditions appeared to change the nature of the species that obtained (by HPLC retention time analyses), with many of the species being unidentifiable. The fact that there are no stable isotopes of astatine, and the chemistry of the nearest halogen iodine is quite different, made it very difficult to interpret results of some experiments. With that said, we believe that a lot of valuable information was obtained from the studies. The research effort evaluated: (1) methods for chemical oxidation of At-211, (2) approaches to chelation of oxidized At-211, and (3) approaches to oxidation of astatophenyl compounds. A major hurdle that had to be surmounted to conduct the research was the development of HPLC conditions to separate and identify the various oxidized species formed. Attempts to develop conditions for separation of iodine and astatine species by normal and reversed-phase TLC and ITLC were not successful. However, we were successful in developing conditions (from a large number of attempts) to separate oxidized forms of iodine ([I-125]iodide, [I-125]iodate and [I-125]periodate) and astatine ([At-211]astatide, [At-211]astatate, [At-211]perastatate, and several unidentified At-211 species). Information on the basic oxidation and characterization of At-211 species is provided under Objective 1. Conditions were developed to obtain new At-211 labeling method where At-211 is chelated with the DOTA and

40 CFR 59.212 - Circumvention.

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2010-07-01

... 40 Protection of Environment 5 2010-07-01 2010-07-01 false Circumvention. 59.212 Section 59.212 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL... Compound Emission Standards for Consumer Products § 59.212 Circumvention. No regulated entity subject to...

19 CFR 212.26 - Determination.

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2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false Determination. 212.26 Section 212.26 Customs... IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Procedures for Considering Applications § 212.26 Determination. The presiding officer shall issue a recommended determination on the application within 90 days after...

19 CFR 212.22 - Reply.

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2010-04-01

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19 CFR 212.01 - Purpose.

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... 19 Customs Duties 3 2010-04-01 2010-04-01 false Purpose. 212.01 Section 212.01 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT General Provisions § 212.01 Purpose. (a) The Equal Access to...

Synthesis and evaluation of tetraphosphonates labelled with 212Bi, 212Pb and 165Er

International Nuclear Information System (INIS)

Hassfjell, S.P.

1997-08-01

The main goal of this work has been to achieve a synthesis of radiolabelled phosphonates for the improvement of diagnostic and therapy of osteoblastic osteosarcoma and sclerotic bone metastases. 212 Bi-DOTMP is shown to be an in vivo stable bone seeking radiopharmaceutical with a potential for a α-particle therapy of the above mentioned diseases. It has biodistribution characteristics similar to 153 Sm-EDTMP, which is now formally approved in several countries, most recently in the US. 212 Pb-DOTMP is also a promising candidate having the advantage of increasing the effective half life of the α-emitter, although loss of some of the in vivo generated 212 Bi may be a problem. A generator has been developed for the production of the α-emitting radionuclide 212 Bi and its parent nuclide 212 Pb. The generator is based on the emanation of 220 Rn from ( 228 Th)barium stearate. The decay product of 220 Rn, 212 Pb deposits on the walls of a polyethylene bottle, and can be wasted off with distilled water. The generator shows no leakage of any long-lived parent nuclides, is easy to operate and has a high degree of radiation safety

19 CFR 212.24 - Settlement.

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2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false Settlement. 212.24 Section 212.24 Customs Duties... IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Procedures for Considering Applications § 212.24 Settlement. The applicant and the Commission may agree on a proposed settlement of the award before final action...

Development and Testing of a 212Pb/212Bi Peptide for Targeting Metastatic Melanoma

Energy Technology Data Exchange (ETDEWEB)

Fisher, Darrell R.

2012-10-25

The purpose of this project is to develop a new radiolabeled peptide for imaging and treating metastatic melanoma. The immunoconjugate consists of a receptor-specific peptide that targets melanoma cells. The beta-emitter lead-212 (half-life = 10.4 hours) is linked by coordination chemistry to the peptide. After injection, the peptide targets melanoma receptors on the surfaces of melanoma cells. Lead-212 decays to the alpha-emitter bismuth-212 (half-life = 60 minutes). Alpha-particles that hit melanoma cell nuclei are likely to kill the melanoma cell. For cancer cell imaging, the lead-212 is replaced by lead-203 (half-life = 52 hours). Lead-203 emits 279 keV photons (80.1% abundance) that can be imaged and measured for biodistribution analysis, cancer imaging, and quantitative dosimetry.

48 CFR 212.211 - Technical data.

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2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Technical data. 212.211 Section 212.211 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... Acquisition of Commercial Items 212.211 Technical data. The DoD policy for acquiring technical data for...

42 CFR 59.212 - Grantee accountability.

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2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Grantee accountability. 59.212 Section 59.212 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.212 Grantee accountability. (a...

22 CFR 212.12 - Effect of nonpublication.

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2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Effect of nonpublication. 212.12 Section 212.12 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Publication in the Federal Register § 212.12 Effect of nonpublication. The materials referenced in § 212.11 shall not be binding upon...

22 CFR 212.21 - Public records.

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2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Public records. 212.21 Section 212.21 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Availability of Information for Public Inspection and Copying § 212.21 Public records. In accordance with this subpart, USAID makes the following...

Studies of 212Pb storm

International Nuclear Information System (INIS)

Yunoki, E.; Kataoka, T.; Michihiro, K.; Sugiyama, H.; Shimizu, M.; Mori, T.

1996-01-01

212 Pb which reached its equilibrium state with its daughters in the air was measured around small uranium mines in Japan. Environmental. 212 Pb concentrations rose suddenly and reached a value ten times as high as usual values. These Phenomena were observed many times during the past six Years. We called these Phenomena 212 Pb storms. Meteorological conditions lead to the variations of 220 Rn progeny concentrations. These phenomena have been studied in the point of meteorology. (author)

25 CFR 212.7 - Environmental studies.

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2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Environmental studies. 212.7 Section 212.7 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF ALLOTTED LANDS FOR MINERAL DEVELOPMENT General § 212.7 Environmental studies. The provisions of § 211.7 of this subchapter...

19 CFR 212.03 - Proceedings covered.

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19 CFR 212.27 - Agency review.

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2010-04-01

... IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Procedures for Considering Applications § 212.27 Agency review... 19 Customs Duties 3 2010-04-01 2010-04-01 false Agency review. 212.27 Section 212.27 Customs... determination and alternative findings of fact and conclusions of law with the Commission. Upon receipt of the...

22 CFR 212.23 - Current index.

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2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Current index. 212.23 Section 212.23 Foreign... Inspection and Copying § 212.23 Current index. USAID maintains and makes available for public inspection and copying a current index providing identifying information for the public as to any matter which has been...

14 CFR 212.7 - Direct sales.

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2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Direct sales. 212.7 Section 212.7... REGULATIONS CHARTER RULES FOR U.S. AND FOREIGN DIRECT AIR CARRIERS § 212.7 Direct sales. (a) Certificated and foreign air carriers may sell or offer for sale, and operate, as principal, Public Charter flights under...

42 CFR 93.212 - Inquiry.

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2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Inquiry. 93.212 Section 93.212 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES HEALTH ASSESSMENTS AND HEALTH EFFECTS STUDIES OF HAZARDOUS SUBSTANCES RELEASES AND FACILITIES PUBLIC HEALTH SERVICE POLICIES ON RESEARCH MISCONDUCT...

22 CFR 212.24 - Effect of noncompliance.

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2010-04-01

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22 CFR 212.13 - Incorporation by reference.

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2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Incorporation by reference. 212.13 Section 212.13 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Publication in the Federal Register § 212.13 Incorporation by reference. For purposes of this subpart B, USAID matters which...

13 CFR 500.212 - Liquidation.

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2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Liquidation. 500.212 Section 500.212 Business Credit and Assistance EMERGENCY OIL AND GAS GUARANTEED LOAN BOARD EMERGENCY OIL AND GAS... proper, consistent with Federal law and regulations. (b) Pursuant to the Guarantee, upon written demand...

29 CFR 2.12 - Audiovisual coverage permitted.

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2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Audiovisual coverage permitted. 2.12 Section 2.12 Labor Office of the Secretary of Labor GENERAL REGULATIONS Audiovisual Coverage of Administrative Hearings § 2.12 Audiovisual coverage permitted. The following are the types of hearings where the Department...

19 CFR 212.21 - Answer to application.

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2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false Answer to application. 212.21 Section 212.21 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Procedures for Considering Applications § 212.21...

19 CFR 212.05 - Standards for awards.

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2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false Standards for awards. 212.05 Section 212.05 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT General Provisions § 212.05 Standards for awards...

19 CFR 212.04 - Eligibility of applicants.

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2010-04-01

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19 CFR 212.10 - Contents of application.

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... 19 Customs Duties 3 2010-04-01 2010-04-01 false Contents of application. 212.10 Section 212.10 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Information Required From Applicants § 212.10...

Synthesis and evaluation of tetraphosphonates labelled with {sup 212}Bi, {sup 212}Pb and {sup 165}Er

Energy Technology Data Exchange (ETDEWEB)

Hassfjell, S.P

1997-08-01

The main goal of this work has been to achieve a synthesis of radiolabelled phosphonates for the improvement of diagnostic and therapy of osteoblastic osteosarcoma and sclerotic bone metastases. {sup 212}Bi-DOTMP is shown to be an in vivo stable bone seeking radiopharmaceutical with a potential for a {alpha}-particle therapy of the above mentioned diseases. It has biodistribution characteristics similar to {sup 153}Sm-EDTMP, which is now formally approved in several countries, most recently in the US. {sup 212}Pb-DOTMP is also a promising candidate having the advantage of increasing the effective half life of the {alpha}-emitter, although loss of some of the in vivo generated {sup 212}Bi may be a problem. A generator has been developed for the production of the {alpha}-emitting radionuclide {sup 212}Bi and its parent nuclide {sup 212}Pb. The generator is based on the emanation of {sup 220}Rn from ({sup 228}Th)barium stearate. The decay product of {sup 220}Rn, {sup 212}Pb deposits on the walls of a polyethylene bottle, and can be wasted off with distilled water. The generator shows no leakage of any long-lived parent nuclides, is easy to operate and has a high degree of radiation safety.

22 CFR 212.32 - Identification of records.

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2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Identification of records. 212.32 Section 212.32 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Access to Agency Records § 212.32 Identification of records. The request for a record by a member of the public must contain a...

7 CFR 1280.212 - Budget and expenses.

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2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Budget and expenses. 1280.212 Section 1280.212... INFORMATION ORDER Lamb Promotion, Research, and Information Order Expenses § 1280.212 Budget and expenses. (a) The Board shall prepare and submit to the Secretary a budget for the fiscal year covering its...

19 CFR 212.11 - Net worth exhibit.

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2010-04-01

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Astatine-211 Radiochemistry: The Development Of Methodologies For High Activity Level Radiosynthesis

International Nuclear Information System (INIS)

Zalutsky, Michael R.

2012-01-01

Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for α-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the α-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At-labeled targeted

ASTATINE-211 RADIOCHEMISTRY: THE DEVELOPMENT OF METHODOLOGIES FOR HIGH ACTIVITY LEVEL RADIOSYNTHESIS

Energy Technology Data Exchange (ETDEWEB)

MICHAEL R. ZALUTSKY

2012-08-08

Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for {alpha}-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the {alpha}-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At

4 CFR 21.2 - Time for filing.

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2010-01-01

... after bid opening or the closing time for receipt of proposals. (b) Protests untimely on their face may... 4 Accounts 1 2010-01-01 2010-01-01 false Time for filing. 21.2 Section 21.2 Accounts GOVERNMENT ACCOUNTABILITY OFFICE GENERAL PROCEDURES BID PROTEST REGULATIONS § 21.2 Time for filing. (a)(1) Protests based...

48 CFR 212.7002 - Pilot program.

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2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Pilot program. 212.7002... OF DEFENSE ACQUISITION PLANNING ACQUISITION OF COMMERCIAL ITEMS Pilot Program for Transition to Follow-On Contracting After Use of Other Transaction Authority 212.7002 Pilot program. ...

48 CFR 871.212 - Contract clauses.

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2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract clauses. 871.212... Rehabilitation and Employment Service 871.212 Contract clauses. Contracting officers must use the following clauses, as appropriate, in solicitations and contracts for vocational rehabilitation and employment...

49 CFR 212.101 - Program principles.

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2010-10-01

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25 CFR 212.56 - Geological and geophysical permits.

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2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Geological and geophysical permits. 212.56 Section 212.56... FOR MINERAL DEVELOPMENT Rents, Royalties, Cancellations, and Appeals § 212.56 Geological and geophysical permits. (a) Permits to conduct geological and geophysical operations on Indian lands which do not...

19 CFR 212.02 - When the Act applies.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false When the Act applies. 212.02 Section 212.02 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT General Provisions § 212.02 When the Act applies...

31 CFR 538.212 - Exempt transactions.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Exempt transactions. 538.212 Section 538.212 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... services to market, produce or co-produce, create or assist in the creation of information and...

40 CFR 86.212-94 - [Reserved

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 18 2010-07-01 2010-07-01 false [Reserved] 86.212-94 Section 86.212-94 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF... Year Gasoline-Fueled New Light-Duty Vehicles, New Light-Duty Trucks and New Medium-Duty Passenger...

22 CFR 212.33 - Procedure for making requests.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Procedure for making requests. 212.33 Section 212.33 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Access to Agency... the Public Reading Room identified in § 212.24(a), may be made by a member of the public in writing...

10 CFR 212.78 - Tertiary incentive crude oil.

Science.gov (United States)

2010-01-01

... 10 Energy 3 2010-01-01 2010-01-01 false Tertiary incentive crude oil. 212.78 Section 212.78 Energy DEPARTMENT OF ENERGY OIL MANDATORY PETROLEUM PRICE REGULATIONS Producers of Crude Oil § 212.78 Tertiary incentive crude oil. Annual prepaid expenses report. By January 31 of each year after 1980, the project...

31 CFR 800.212 - Foreign entity.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Foreign entity. 800.212 Section 800.212 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... business is outside the United States or its equity securities are primarily traded on one or more foreign...

7 CFR 1400.212 - Growers of hybrid seed.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Growers of hybrid seed. 1400.212 Section 1400.212... AND SUBSEQUENT CROP, PROGRAM, OR FISCAL YEARS Payment Eligibility § 1400.212 Growers of hybrid seed. The existence of a hybrid seed contract for a person or legal entity will not be taken into account...

22 CFR 212.11 - Materials to be published.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Materials to be published. 212.11 Section 212... Federal Register § 212.11 Materials to be published. (a) USAID separately states and currently publishes... section. (b) USAID Public Notice No. 1 and the USAID Regulations published in chapter II of Title 22 and...

8 CFR 212.9 - Applicability of section 212(a)(32) to certain derivative third and sixth preference and...

Science.gov (United States)

2010-01-01

... certain derivative third and sixth preference and nonpreference immigrants. 212.9 Section 212.9 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS DOCUMENTARY REQUIREMENTS: NONIMMIGRANTS... certain derivative third and sixth preference and nonpreference immigrants. A derivative beneficiary who...

12 CFR 212.6 - General exemption.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 2 2010-01-01 2010-01-01 false General exemption. 212.6 Section 212.6 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM MANAGEMENT... women; (3) Is a depository institution that has been chartered for less than two years; or (4) Is deemed...

Biodistribution of 212Pb Conjugated Trastuzumab in Mice

International Nuclear Information System (INIS)

Schneider, N.; Lobaugh, M.; Sandwall, P.; Glover, S.; Murry, M.; Dong, Z.; Spitz, H.

2014-01-01

Clinical use of radiolabeled monoclonal antibodies in therapeutic treatment of cancer is increasing. This study demonstrates an increased uptake rate in the tumor over a 72 hr period of observation following a single intravenous injection of 212Pb-trastuzumab in mice. Whereas 212Pb-trastuzumab appeared not to cause systemic toxicity4, there may be concomitant uptake in other organs that should be considered in evaluating the risk of radiation toxicity associated with therapy. Additional laboratory and clinical study with 212Pb-trastuzumab should be conducted to define an optimized therapeutic strategy and determine the radiation doses delivered to non-targeted organs and tissues using microdosimetry methods. Results of this biodistribution study support further investigation of radiolabeled 212Pb-TCMC-trastuzumab, radiobiological organ microdosimetry, and optimal dosing regimens for 212Pb-trastuzumab as a therapeutic agent

Study of in vivo generators Pb-212/Bi--212 and U-230/Th-226 for alpha radioimmunotherapy

International Nuclear Information System (INIS)

Le Du, A.

2011-01-01

Alpha-radioimmunotherapy is a promising cancer therapy that uses a-particles vectorized by monoclonal antibody to break down cancerous tumors. The notion of in vivo generator was introduced in 1989 by Leonard Mausner. The concept involves labeling of various molecular carriers (antibodies, peptides, etc) with intermediate half-life generator parents, which after accumulation in the desired tissue generate much shorter half-life daughter radionuclide. This thesis focuses on the study of two in vivo generators potentially interesting for alpha-radioimmunotherapy: Pb-212 / Bi-212 generator and U-230 / Th-226 generator. The first part of this work presents the Pb-212 / Bi-212 generator, two approaches allowing the vectorization. Chelation approach on a protein and an approach by encapsulation in liposomes have been proposed. This last approach appears to be the most interesting. In vitro stability studies have been performed on these labeling. The second part of this work presents the U-230 / Th-226 generator. Studies have first been made to achieve a theoretical model to describe the speciation of Th(IV) in human serum. The efficacy of DTPA as chelating agent for complexation of Th(IV) in human serum could thus be estimated. (author)

45 CFR 212.7 - Repayment to the United States.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 2 2010-10-01 2010-10-01 false Repayment to the United States. 212.7 Section 212... UNITED STATES CITIZENS RETURNED FROM FOREIGN COUNTRIES § 212.7 Repayment to the United States. (a) An..., any or all of the cost of such assistance to the United States, except insofar as it is determined...

25 CFR 212.47 - Diligence, drainage and prevention of waste.

Science.gov (United States)

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Diligence, drainage and prevention of waste. 212.47 Section 212.47 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF ALLOTTED LANDS FOR MINERAL DEVELOPMENT Rents, Royalties, Cancellations, and Appeals § 212.47 Diligence...

Comment: 212 [Taxonomy Icon

Lifescience Database Archive (English)

Full Text Available Oryzias javanicus Oryzias javanicus Oryzias_javanicus_L.png 212.png Kiyoshi Naruse ...(National Institute for Basic Biology) licensed under CC Attribution2.1 Japan ジャワメダカ (Oryzias javanicus) インド

12 CFR 212.1 - Authority, purpose, and scope.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Authority, purpose, and scope. 212.1 Section... MANAGEMENT OFFICIAL INTERLOCKS § 212.1 Authority, purpose, and scope. (a) Authority. This part is issued... competition by generally prohibiting a management official from serving two nonaffiliated depository...

22 CFR 212.31 - Availability of agency records.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Availability of agency records. 212.31 Section 212.31 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Access to Agency... Federal Register pursuant to subpart B; (b) Matters made available to the public pursuant to subpart C...

22 CFR 212.22 - Protection of personal privacy.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Protection of personal privacy. 212.22 Section... Information for Public Inspection and Copying § 212.22 Protection of personal privacy. To the extent required to prevent a clearly unwarranted invasion of personal privacy, USAID may delete identifying details...

5 CFR 179.212 - Procedures for salary offset.

Science.gov (United States)

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Procedures for salary offset. 179.212... COLLECTION STANDARDS Salary Offset § 179.212 Procedures for salary offset. (a) The Director or his or her designee shall coordinate salary deductions under this subpart. (b) OPM payroll office shall determine the...

22 CFR 212.38 - Predisclosure notification procedures for confidential commercial information.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Predisclosure notification procedures for confidential commercial information. 212.38 Section 212.38 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Access to Agency Records § 212.38 Predisclosure notification procedures for...

49 CFR 212.107 - Certification.

Science.gov (United States)

2010-10-01

..., DEPARTMENT OF TRANSPORTATION STATE SAFETY PARTICIPATION REGULATIONS State/Federal Roles § 212.107... State agency will, at a minimum, conduct planned compliance inspections meeting the level of effort...

49 CFR 212.105 - Agreements.

Science.gov (United States)

2010-10-01

... TRANSPORTATION STATE SAFETY PARTICIPATION REGULATIONS State/Federal Roles § 212.105 Agreements. (a) Scope. The... facilities, equipment, and operating practices through planned routine compliance inspections for all, or a...

22 CFR 212.25 - Procedures for obtaining materials under this subpart.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Procedures for obtaining materials under this subpart. 212.25 Section 212.25 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Availability of Information for Public Inspection and Copying § 212.25 Procedures for obtaining materials under...

8 CFR 212.5 - Parole of aliens into the United States.

Science.gov (United States)

2010-01-01

... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Parole of aliens into the United States. 212.5 Section 212.5 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS DOCUMENTARY REQUIREMENTS: NONIMMIGRANTS; WAIVERS; ADMISSION OF CERTAIN INADMISSIBLE ALIENS; PAROLE § 212.5...

36 CFR 212.56 - Identification of designated roads, trails, and areas.

Science.gov (United States)

2010-07-01

... roads, trails, and areas. 212.56 Section 212.56 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle Use § 212.56 Identification of designated roads, trails, and areas. Designated roads, trails, and areas...

20 CFR 410.212 - Conditions of entitlement; child.

Science.gov (United States)

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Conditions of entitlement; child. 410.212...; Filing of Claims and Evidence § 410.212 Conditions of entitlement; child. (a) An individual is entitled to benefits if such individual: (1) Is the child or stepchild (see § 410.330) of (i) a deceased miner...

Spectroscopy of 214Bi and systematics of 210,212,214Pb(0+) long->β-210,212,214Bi(0-)

International Nuclear Information System (INIS)

Berant, Z.; Schuhmann, R.B.; Alburger, D.E.; Chou, W.T.; Gill, R.L.; Warburton, E.K.; Wesselborg, C.

1991-01-01

Experiments designed to provide more information on the spectroscopy of 214 Bi, and on 214 Pb(β - ) 214 Bi in particular, were undertaken because of interest in first-forbidden β decay in the lead region. The experiments consisted of γ-γ coincidences and angular correlations, conversion electron measurements, level lifetime determinations, and precision γ-ray energy measurements. The 352-keV level of 214 Bi was found to be a strong candidate (and the only candidate) for the 0 1 - state. Recent additions to the 214 Pb decay scheme are confirmed by γ-γ coincidence measurements. A careful evaluation of the 214 Bi level scheme is made with emphasis on separating experimentally based conclusions from speculations based on systematics and other ''weak'' arguments. Shell-model calculations of the spectroscopy of 210,212 Bi and 210,212 Pb(β - ) 210,212 Bi were performed using a modification of the Kuo-Herling realistic interaction. These calculations and a generalized seniority model provide a basis for an examination of the systematics of the A=210, 212, 214 spectroscopy and β decay. The generalized seniority model is found to be a quite good approximation which provides a quantitative understanding of the 214 Pb decay rates

22 CFR 212.42 - Exemption from 5 U.S.C. 552.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Exemption from 5 U.S.C. 552. 212.42 Section 212.42 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Exemptions From Disclosure § 212.42 Exemption from 5 U.S.C. 552. Whenever a request is made which involves access to records...

48 CFR 212.7003 - Technical data and computer software.

Science.gov (United States)

2010-10-01

... computer software. 212.7003 Section 212.7003 Federal Acquisition Regulations System DEFENSE ACQUISITION... data and computer software. For purposes of establishing delivery requirements and license rights for technical data under 227.7102 and for computer software under 227.7202, there shall be a rebuttable...

21 CFR 212.10 - What personnel and resources must I have?

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What personnel and resources must I have? 212.10 Section 212.10 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES.... 12-12-2011) Personnel and Resources § 212.10 What personnel and resources must I have? You must have...

19 CFR 212.12 - Documentation of fees and expenses.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false Documentation of fees and expenses. 212.12 Section 212.12 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Information Required From...

19 CFR 212.20 - Filing and service of documents.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false Filing and service of documents. 212.20 Section 212.20 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Procedures for Considering...

Aptamer-miRNA-212 Conjugate Sensitizes NSCLC Cells to TRAIL

Directory of Open Access Journals (Sweden)

Margherita Iaboni

2016-01-01

Full Text Available TNF-related apoptosis-inducing ligand (TRAIL is a promising antitumor agent for its remarkable ability to selectively induce apoptosis in cancer cells, without affecting the viability of healthy bystander cells. The TRAIL tumor suppressor pathway is deregulated in many human malignancies including lung cancer. In human non-small cell lung cancer (NSCLC cells, sensitization to TRAIL therapy can be restored by increasing the expression levels of the tumor suppressor microRNA-212 (miR-212 leading to inhibition of the anti-apoptotic protein PED/PEA-15 implicated in treatment resistance. In this study, we exploited a previously described RNA aptamer inhibitor of the tyrosine kinase receptor Axl (GL21.T expressed on lung cancer cells, as a means to deliver miR-212 into human NSCLC cells expressing Axl. We demonstrate efficient delivery of miR-212 following conjugation of the miR to GL21.T (GL21.T-miR212 chimera. We show that the chimera downregulates PED and restores TRAIL-mediate cytotoxicity in cancer cells. Importantly, treatment of Axl+ lung cancer cells with the chimera resulted in (i an increase in caspase activation and (ii a reduction of cell viability in combination with TRAIL therapy. In conclusion, we demonstrate that the GL21.T-miR212 chimera can be employed as an adjuvant to TRAIL therapy for the treatment of lung cancer.

Clearance of lead-212 ions from rabbit bronchial epithelium to blood

International Nuclear Information System (INIS)

James, A.C.; Greenhalgh, J.R.; Smith, H.

1977-01-01

The absorption of 212 Pb ions from bronchial epithelium to blood has been investigated in anaesthetized rabbits. The 212 Pb ions were introduced by intubation either into the trachea or into smaller, more distal bronchi. Removal from lung was followed by external γ-counting. Mucociliary clearance to the GI tract was blocked by tracheostomy. Two distinct phases of clearance from bronchial epithelium to blood were observed. Approximately 20% of deposited 212 Pb was rapidly absorbed with a half-time of about 4 min, the remainder with a biological half-time of about 9 h, irrespective of the site of instillation in the bronchial tree. Two hours after deposition, the 212 Pb remaining in lung was found to be partitioned between mucus and the bronchial epithelium, with a substantial but minor fraction in the epithelium. Uptake of 212 Pb in the skeleton was estimated to be about 20% of the 212 Pb entering the blood circulation. Removal by the kidneys, at 25%, was comparable with skeletal uptake. These results are compared with previously published work using rodents, dogs and man which demonstrated either rapid or slow absorption but not both phases occurring together. (author)

31 CFR 0.212 - Influencing legislation or petitioning Congress.

Science.gov (United States)

2010-07-01

... petitioning Congress. 0.212 Section 0.212 Money and Finance: Treasury Office of the Secretary of the Treasury... petitioning Congress. (a) Employees shall not use Government time, money, or property to petition a Member of Congress to favor or oppose any legislation. This prohibition does not apply to the official handling...

37 CFR 212.5 - Recordation of distinctive identification of vessel hull designer.

Science.gov (United States)

2010-07-01

... identification of vessel hull designer. 212.5 Section 212.5 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES PROTECTION OF VESSEL HULL DESIGNS § 212.5 Recordation of distinctive identification of vessel hull designer. (a) General. Any owner of a vessel hull may...

36 CFR 212.55 - Criteria for designation of roads, trails, and areas.

Science.gov (United States)

2010-07-01

... roads, trails, and areas. 212.55 Section 212.55 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle Use § 212.55 Criteria for designation of roads, trails, and areas. (a) General criteria for designation of...

49 CFR 212.115 - Enforcement actions.

Science.gov (United States)

2010-10-01

..., DEPARTMENT OF TRANSPORTATION STATE SAFETY PARTICIPATION REGULATIONS State/Federal Roles § 212.115 Enforcement... assess and compromise penalties, to issue emergency orders and compliance orders, institute or cause to...

18 CFR 157.212 - Synthetic and liquefied natural gas facilities.

Science.gov (United States)

2010-04-01

... natural gas facilities. 157.212 Section 157.212 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER NATURAL GAS ACT APPLICATIONS FOR CERTIFICATES... 7 OF THE NATURAL GAS ACT Interstate Pipeline Blanket Certificates and Authorization Under Section 7...

5 CFR 591.212 - How does OPM select survey items?

Science.gov (United States)

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false How does OPM select survey items? 591.212 Section 591.212 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS ALLOWANCES AND DIFFERENTIALS Cost-of-Living Allowance and Post Differential-Nonforeign Areas Cost-Of-Living...

19 CFR 212.13 - When an application may be filed.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false When an application may be filed. 212.13 Section 212.13 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Information Required From...

36 CFR 212.10 - Maximum economy National Forest System roads.

Science.gov (United States)

2010-07-01

... Forest System roads. 212.10 Section 212.10 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT... economy National Forest System roads. The Chief may acquire, construct, reconstruct, improve, and maintain National Forest System roads within and near the National Forests and other lands administered by the...

22 CFR 212.37 - Procedures for agency consideration of appeals.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Procedures for agency consideration of appeals. 212.37 Section 212.37 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Access... of the decisions and of the provisions for judicial review of the Agency's denial of the request. (c...

36 CFR 212.9 - Principles for sharing use of roads.

Science.gov (United States)

2010-07-01

... roads. 212.9 Section 212.9 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE... of roads. The use of roads under arrangements for sharing costs or performance shall be in accordance with the following: (a) Road improvement. Use of a road for commercial hauling, except occasional or...

36 CFR 212.1 - Definitions.

Science.gov (United States)

2010-07-01

... the National Forest System. Area. A discrete, specifically delineated space that is smaller, and in... vehicle use pursuant to § 212.51 on a motor vehicle use map. Federal airport funds. Discretionary funds... including surface and shoulders, parking and side areas, structures, and such traffic-control devices as are...

7 CFR 330.212 - Movement of plant pests by baggage.

Science.gov (United States)

2010-01-01

... 7 Agriculture 5 2010-01-01 2010-01-01 false Movement of plant pests by baggage. 330.212 Section... INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FEDERAL PLANT PEST REGULATIONS; GENERAL; PLANT PESTS; SOIL, STONE, AND QUARRY PRODUCTS; GARBAGE Movement of Plant Pests § 330.212 Movement of plant pests by baggage...

25 CFR 212.46 - Inspection of premises, books, and accounts.

Science.gov (United States)

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Inspection of premises, books, and accounts. 212.46 Section 212.46 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF... premises, books, and accounts. The provisions of § 211.46 of this subchapter are applicable to leases under...

The tribology of PS212 coatings and PM212 composites for the lubrication of titanium 6Al-4V components of a Stirling engine space power system

Science.gov (United States)

Sliney, Harold E.; Lukaszewicz, Victor; Dellacorte, Christopher

1994-01-01

The Stirling space power machine incorporates a linear alternator to generate electrical power. The alternator is a reciprocating device that is driven by a solar or nuclear-powered Stirling engine. The power piston and cylinder are made of titanium 6Al-4V (Ti6-4) alloy, and are designed to be lubricated by a hydrodynamically-generated gas film. Rubbing occurs during starts and stops and there is the possibility of an occasional high speed rub. Since titanium is known to have a severe galling tendency in sliding contacts, a 'back-up', self-lubricating coating on the cylinder and/or the piston is needed. This report describes the results of a research program to study the lubrication of Ti6-4 with the following chromium carbide based materials: plasma-sprayed PS212 coatings and sintered PM212 counterfaces. Program objectives are to achieve adherent coatings on Ti6-4 and to measure the friction and wear characteristics of the following sliding combinations under conditions simulative of the Stirling-driven space power linear alternator: Ti6-4/Ti6-4 baseline, Ti6-4/PS212-coated Ti6-4, and PS212-coated Ti6-4/PM212.

29 CFR 778.212 - Gifts, Christmas and special occasion bonuses.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Gifts, Christmas and special occasion bonuses. 778.212... COMPENSATION Payments That May Be Excluded From the âRegular Rateâ Bonuses § 778.212 Gifts, Christmas and... “regular rate” shall not be deemed to include “sums paid as gifts; payments in the nature of gifts made at...

19 CFR 212.07 - Rulemaking on maximum rates for attorney fees.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false Rulemaking on maximum rates for attorney fees. 212.07 Section 212.07 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT General Provisions...

48 CFR 12.212 - Computer software.

Science.gov (United States)

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Computer software. 12.212... software. (a) Commercial computer software or commercial computer software documentation shall be acquired... required to— (1) Furnish technical information related to commercial computer software or commercial...

The tribology of PS212 coatings and PM212 composites for the lubrication of titanium 6A1-4V components of a Stirling engine space power system

Science.gov (United States)

Sliney, Harold E.; Dellacorte, Christopher; Lukaszewicz, Victor

1995-01-01

The Stirling space power machine incorporates a linear alternator to generate electrical power. The alternator is a reciprocating device that is driven by a solar or nuclear-powered Stirling engine. The power piston and cylinder are made of titanium 6A1-4V (Ti6-4) alloy, and are designed to be lubricated by a hydrodynamically-generated gas film. Rubbing occurs during starts and stops and there is a possibility of an occasional high speed rub. Since titanium is known to have a severe galling tendency in sliding contacts, a 'backup,' self-lubricating coating on the cylinder and/or the piston is needed. This report describes the results of a research program to study the lubrication of Ti6-4 with the following chromium carbide based materials: plasma-sprayed PS212 coatings and sintered PM212 counterfaces. Program objectives are to achieve adherent coatings on Ti6-4 and to measure the friction and wear characteristics of the following sliding combinations under conditions simulative of the Stirling-driven space power linear alternator: Ti6-4/Ti6-4 baseline, Ti6-4/PS212 coated Ti6-4, and Ps212 coated Ti6-4/PM212

Tribology and Microstructure of PS212 with a Cr2O3 Seal Coat

Science.gov (United States)

Sliney, Harold E.; Benoy, Patricia A.; Korenyi-Both, Andras; Dellacorte, Christopher

1994-01-01

PS212 is a plasma sprayed metal bonding chrome carbide coating with solid lubricant additives which has lubricating properties at temperatures up to about 900 deg C. The coating is diamond ground to achieve an acceptable tribological surface. But, as with many plasma spray coatings, PS212 is not fully-dense. In this study, a chromium oxide base seal coating is used in an attempt to seal any porosity that is open to the surface of the PS212 coating, and to study the effect of the sealant on the tribological properties of PS212. The results indicate that the seal coating reduces friction and wear when it is applied and then diamond ground leaving a thin layer of seal coating which fills in the surface pits of the PS212 coating.

50 CFR 216.212 - Effective dates.

Science.gov (United States)

2010-10-01

..., DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE MAMMALS Taking of Marine Mammals Incidental to Explosive Severance Activities Conducted During Offshore Structure Removal Operations on the Outer Continental Shelf in the U.S. Gulf of Mexico § 216.212 Effective dates...

Dosimetric assessment from 212Pb inhalation at a thorium purification plant

International Nuclear Information System (INIS)

Campos, M. P.; Pecequilo, B. R. S.

2004-01-01

At the Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo (Brazil)), there is a facility (thorium purification plant) where materials with high thorium concentrations are manipulated. In order to estimate afterwards the lung cancer risk for the workers, the thoron daughter ( 212 Pb) levels were assessed and the committed effective and lung committed equivalent doses for workers in place. A total of 28 air filter samples were measured by total alpha counting through the modified Kusnetz method, to determine the 212 Pb concentration. The committed effective dose and lung committed equivalent dose due to 212 Pb inhalation were derived from compartmental analysis following the ICRP 66 lung compartmental model, and ICRP 67 lead metabolic model. (authors)

27 CFR 28.212 - Persons authorized.

Science.gov (United States)

2010-04-01

... OF THE TREASURY LIQUORS EXPORTATION OF ALCOHOL Exportation of Wine With Benefit of Drawback § 28.212... plants, bonded wine cellars, or taxpaid wine bottling houses, and persons who are wholesale liquor... accordance with part 31 of this chapter, are authorized to remove wines under the provisions of this subpart...

37 CFR 212.3 - Registration of claims for protection of eligible designs.

Science.gov (United States)

2010-07-01

... color as well as color contrast. (ii) The use of broken lines in drawings depicting the design is... design. (4) Photographs. High quality black and white or color photographs will be accepted provided that... protection of eligible designs. 212.3 Section 212.3 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE...

Skeletal 212Pb retention following 224Ra injection: extrapolation of animal data to adult humans

International Nuclear Information System (INIS)

Schlenker, R.A.

1988-01-01

Two methods of interspecies extrapolation, one based on a correlation of skeletal 212 Pb/ 224 Ra with body weight, the other based on the mechanistic relationship between skeletal 212 Pb/ 224 Ra and reciprocal bone surface-to-volume ratio, lead to the conclusion that the retention of 212 Pb in the adult human skeleton is approximately complete a few days after injection. The correlation-based method gives most probable values for 212 Pb/ 224 Ra of 1.0 and 1.1 at 2 d and 7 d after injection, compared with values of 1.05 and 1.27 expected at these same times if the retention of 212 Pb were complete from the time of injection and if no 212 Pb were in the injection solution. The range of values corresponding to one geometric standard error on either side of the most probable value is 0.87 to 1.21 at 2 d post-injection. With the method based on the reciprocal bone surface-to-volume ratio, the best estimate of 212 Pb/ 224 Ra at 2 d after injection is 0.88, equal to the value observed in young adult beagles. An alternative interpretation of the results of this latter method leads to the conclusion that retention is complete, with 212 Pb/ 224 Ra equal to 1.0 for a 212 Pb-free injection solution and 1.1 for a solution containing 212 Pb in secular equilibrium with 224 Ra. This work, which uses 224 Ra daughter product retention data from mice, rats and dogs following 224Ra injection, provides a scientific foundation for retention assumptions made in the calculation of mean skeletal dose for adult humans. There now appear to be few uncertainties in these latter dose values, stemming from inaccurate retention assumptions; but substantial uncertainties remain in the mean skeletal dose values for juveniles and in the endosteal tissue doses regardless of age

SU-C-201-05: Imaging 212Pb-TCMC-Trastuzumab for Alpha Radioimmunotherapy for Ovarian Cancer

Energy Technology Data Exchange (ETDEWEB)

Shen, S; Meredith, R; Azure, M; Yoder, D [University of Alabama, Birmingham, AL (United States); Torgue, J; Banaga, E [AREVA Med LLC, Bethesda, MD (United States)

2015-06-15

Purpose: To support the phase I trial for toxicity, biodistribution and pharmacokinetics of intra-peritoneal (IP) 212Pb-TCMC-trastuzumab in patients with HER-2 expressing malignancy. A whole body gamma camera imaging method was developed for estimating amount of 212Pb-TCMC-trastuzumab left in the peritoneal cavity. Methods: {sup 212}Pb decays to {sup 212}Bi via beta emission. {sup 212}Bi emits an alpha particle at an average of 6.1 MeV. The 238.6 keV gamma ray with a 43.6% yield can be exploited for imaging. Initial phantom was made of saline bags with 212Pb. Images were collected for 238.6 keV with a medium energy general purpose collimator. There are other high energy gamma emissions (e.g. 511keV, 8%; 583 keV, 31%) that penetrate the septae of the collimator and contribute scatter into 238.6 keV. An upper scatter window was used for scatter correction for these high energy gammas. Results: A small source containing 212Pb can be easily visualized. Scatter correction on images of a small 212Pb source resulted in a ∼50% reduction in the full width at tenth maximum (FWTM), while change in full width at half maximum (FWHM) was <10%. For photopeak images, substantial scatter around phantom source extended to > 5 cm outside; scatter correction improved image contrast by removing this scatter around the sources. Patient imaging, in the 1st cohort (n=3) showed little redistribution of 212Pb-TCMC-trastuzumab out of the peritoneal cavity. Compared to the early post-treatment images, the 18-hour post-injection images illustrated the shift to more uniform anterior/posterior abdominal distribution and the loss of intensity due to radioactive decay. Conclusion: Use of medium energy collimator, 15% width of 238.6 keV photopeak, and a 7.5% upper scatter window is adequate for quantification of 212Pb radioactivity inside peritoneal cavity for alpha radioimmunotherapy of ovarian cancer. Research Support: AREVA Med, NIH 1UL1RR025777-01.

49 CFR 212.109 - Joint planning of inspections.

Science.gov (United States)

2010-10-01

... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION STATE SAFETY PARTICIPATION REGULATIONS State/Federal Roles § 212.109... designed to monitor the compliance of the railroads, shippers, and manufacturers operating within the State...

{sup 212}Pb as tracer for PM deposition on urban vegetation

Energy Technology Data Exchange (ETDEWEB)

Voltaggio, Mario; Spadoni, Massimo; Carloni, Serena; Guglietta, Daniela

2016-11-01

{sup 212}Pb concentration in outdoor air is closely correlated with fine suspended particulate matter in the atmosphere. Thanks to this association, this isotope can be used to trace the sinking processes of particulate matter due to the vegetation, also providing accurate estimations of the deposition velocity on foliar surfaces. This approach is particularly effective in areas with high thoron fluxes and, consequently, high {sup 212}Pb fluxes from soil. The contribution of vegetation to the improvement of air quality (AQImp) in the municipality area (MA) of Rome (Latium, Italy), almost entirely located on Th-enriched volcanic soils, was estimated by studying {sup 212}Pb deposition velocity on the grasses (0.9–2.5 mm·s{sup −} {sup 1}) and on the most common tree classes, namely conifers (1.5–15 mm·s{sup −} {sup 1}), evergreen (1–4 mm·s{sup −} {sup 1}) and deciduous (0.2–1.5 mm·s{sup −} {sup 1}). {sup 212}Pb activity in outdoor air was determined by gamma spectrometry after air pumping with accumulation on cellulose filters and after collection on artificial electrostatically charged surfaces (ECS). The high {sup 212}Pb activity values obtained from this analysis (0.90 ± 0.6 Bq m{sup −} {sup 3} and 0.58 ± 0.15 Bq m{sup −} {sup 3}, respectively near and far from the soil) are consistent both with the average regional thoron flux from volcanic soils (2.9 · 10{sup 4} Bq m{sup −} {sup 2}·h{sup −} {sup 1}) and with the thoron flux measured in the volcanic soils of the study area. Thoron and {sup 212}Pb fluxes were also measured both in laboratory and in the field under different soil moisture conditions. The total AQImp for the period from September 2014 to September 2015, calculated after the classification of the MA of Rome into six classes of vegetation, provided a value of 0.20 corresponding to 2.3 Tons per day of removed PM{sub 10}. The role of grasslands in the PM{sub 10} removal, the contribution of the vegetation to the

The low lying yrast structure of 212Po

International Nuclear Information System (INIS)

Poletti, A.R.; Dracoulis, G.D.; Byrne, A.P.; Stuchbery, A.E.

1987-07-01

The properties of states in 212 Po populated by the 208 Pb( 9 Be,αn) 212 Po reaction have been investigated. the previously proposed yrast scheme below 3MeV has been verified and eight further transitions placed in the level scheme. Mean lives of the 6 + , 8 + and (10 + ) states at 1355, 1476 and 1834 keV have been measured as 1.1(3), 24.6(3) and 0.8(2)ns respectively. Shell model calculations using effective interactions were used to interpret the observed scheme. A synthesis of all available theoretical and experimental evidence allows the 65s isomer to be identified clearly as the 18 + level arising primarily from the π(h 9/2 2 )*ν(g 9/2 i 11/2 ) configuration

48 CFR 212.504 - Applicability of certain laws to subcontracts for the acquisition of commercial items.

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2010-10-01

... laws to subcontracts for the acquisition of commercial items. 212.504 Section 212.504 Federal... PLANNING ACQUISITION OF COMMERCIAL ITEMS Applicability of Certain Laws to the Acquisition of Commercial Items 212.504 Applicability of certain laws to subcontracts for the acquisition of commercial items. (a...

22 CFR 212.41 - Exemptions from publication and disclosure requirements of subparts B, C, and D.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Exemptions from publication and disclosure requirements of subparts B, C, and D. 212.41 Section 212.41 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Exemptions From Disclosure § 212.41 Exemptions from publication and disclosure...

36 CFR 212.2 - Forest transportation program.

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2010-07-01

... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Forest transportation program... TRAVEL MANAGEMENT Administration of the Forest Transportation System § 212.2 Forest transportation... public at the headquarters of that administrative unit. (b) Forest transportation atlas. A forest...

Spectroscopy of 212Rn

International Nuclear Information System (INIS)

Stuchbery, A.E.; Dracoulis, G.D.; Byrne, A.P.; Poletti, A.R.

1988-01-01

Excited states of 212 Rn have been studied using γ-ray and electron spectroscopy following the reactions 208 Pb( 9 Be, 5n) and 204 Hg( 13 C,5n). With the exception of the energy of the yrast 8 + → 6 + transition, the previously proposed level scheme has been verified. New transitions have been placed in the level scheme and new lifetime and g-factor results obtained. The level scheme and electromagnetic properties of selected isomeric states are compared with the results of shell model and semi-empirical shell-model calculations, including coupling to octupole vibrations. (orig.)

Spectroscopy of 212Rn

International Nuclear Information System (INIS)

Stuchbery, A.E.; Dracoulis, G.D.; Byrne, A.P.; Poletti, A.R.

1988-06-01

Excited states of 212 Rn have been studied using γ-ray and electron spectroscopy following the reactions 208 Pb ( 9 Be,5n) and 204 Hg( 13 C,5n). With the exception of the energy of the yrast 8 + → 6 + transition, the previously proposed level scheme has been verified. New transitions have been placed in the level scheme and new lifetime and g-factor results obtained. The level scheme and electromagnetic properties of selected isomeric states are compared with the results of shell model and semi-empirical shell-model calculations, including coupling to octupole vibrations

20 CFR 702.212 - Notice; when given; when given for certain occupational diseases.

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2010-04-01

... occupational diseases. 702.212 Section 702.212 Employees' Benefits EMPLOYMENT STANDARDS ADMINISTRATION... occupational diseases. (a) For other than occupational diseases described in (b), the employee must give notice...). (b) In the case of an occupational disease which does not immediately result in disability or death...

25 CFR 212.6 - Authority and responsibility of the Minerals Management Service (MMS).

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2010-04-01

... Service (MMS). 212.6 Section 212.6 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND... the Minerals Management Service (MMS). The functions of the MMS for reporting, accounting, and... satisfactorily addressing the functions governed by MMS regulations, the Secretary may approve alternate...

47 CFR 90.212 - Provisions relating to the use of scrambling devices and digital voice modulation.

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2010-10-01

... devices and digital voice modulation. 90.212 Section 90.212 Telecommunication FEDERAL COMMUNICATIONS... Standards § 90.212 Provisions relating to the use of scrambling devices and digital voice modulation. (a... techniques or digital voice modulation requires the specific authorization of F1E or G1E emission, and these...

Novel gene PUS3 c.A212G mutation in Ukrainian family with intellectual disability

Directory of Open Access Journals (Sweden)

Gulkovskyi R. V.

2015-04-01

Full Text Available Aim. To evaluate a possible role of a novel c.A212G substitution in the PUS3 gene at intellectual disability (ID. Methods. The observed group consisted of the ID Ukrainian family members (parents and two affected children and the control group – of 300 healthy individuals from general population of Ukraine. Sanger sequencing of the PUS3 gene exon 1 was performed for the family members. Polymorphic variants of c.A212G were analyzed using ARMS PCR. The homology models of wild type and p.Y71C mutant catalytic domains of human Pus3 were generated using the crystal structure of the human Pus1 catalytic domain (PDB ID: 4NZ6 as a template. Results. It was shown that the father of the affected siblings was the c.A212G substitution heterozygous carrier whereas the mother was a wild type allele homozygote, and the exom sequencing result was confirmed – the affected children are 212G homozygotes. We supposed de novo mutation in the maternal germ line. A low frequency of 212G allele (0.0017 was shown in the population of Ukraine. Homology modelling of the wild type and p.Y71C mutant catalytic domain of human Pus3 revealed that substitution p.Y71C is located in close proximity to its active site. Conclusions. The absence of hypoproteinemia in our patients, homozygous for the 212C allele allows us to assume that the mutation c.A212G PUS3 is rather neutral and cannot be the major cause of ID. However, considering a low frequency of the 212G allele in the population and close localization of p.Y71C substitution to the active site of hPus3 we cannot exclude that the c.A212G mutation in PUS3 may be a modifier for some pathologies including syndromic ID.

The low-lying yrast structure of 212Po

International Nuclear Information System (INIS)

Poletti, A.R.; Dracoulis, G.D.; Byrne, A.P.; Stuchbery, A.E.

1987-01-01

The properties of states in 212 Po populated by the 208 Pb( 9 Be,αn) 212 Po reaction have been investigated. The previously proposed yrast scheme below ≅ 3 MeV has been verified and eight further transitions placed in the level scheme. Mean lives of the 6 + , 8 + and (10 + ) states at 1355, 1476 and 1834 keV have been measured at 1.1 (3), 24.6 (3) and 0.8 (2) ns respectively. Shell model calculations using effective interactions were used to interpret the observed scheme. A synthesis of all available theoretical and experimental evidence allows the τ m = 65 s isomer to be identified clearly as the 18 + level arising primarily from the π(h 2 9/2 ) * ν(g 9/2 i 11/2 ) configuration. (orig.)

Diagnostic and prognostic potential of serum miR-132/212 cluster in patients with hepatocellular carcinoma.

Science.gov (United States)

Wang, Feng; Wang, Jun; Ju, Linlin; Chen, Lin; Cai, Weihua; Yang, Jialin

2018-01-01

Background It has been reported that both of the miR-132/212 (micro-RNA) cluster members, miR-132 and miR-212, are downregulated in hepatocellular carcinoma. Nevertheless, the expression pattern and clinical utility of serum miR-132/212 in hepatocellular carcinoma are still unknown. Methods In this study, serum concentrations of miR-132 and miR-212 were measured in 80 hepatocellular carcinoma patients, 51 controls with chronic liver diseases and 42 healthy volunteers by using quantitative real-time polymerase chain reaction. Results In hepatocellular carcinoma patients, serum concentrations of miR-132 and miR-212 were significantly reduced and strongly correlated (r = 0.603, p hepatocellular carcinoma. Moreover, the combination of serum miR-132, miR-212 and alpha-fetoprotein improved the diagnostic efficiency for hepatocellular carcinoma, especially in sensitivity and negative predictive value. Serum miR-132 was associated with tumour differentiation degree ( p = 0.021) and tumour-node-metastasis stage ( p = 0.002); serum miR-212 correlated with tumour size ( p = 0.023) and tumour-node-metastasis stage ( p = 0.007). Kaplan-Meier analyses indicated poorer overall survival in hepatocellular carcinoma patients with lower serum concentrations of miR-132 ( p hepatocellular carcinoma.

21 CFR 212.80 - What are the requirements associated with labeling and packaging PET drug products?

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What are the requirements associated with labeling and packaging PET drug products? 212.80 Section 212.80 Food and Drugs FOOD AND DRUG ADMINISTRATION... POSITRON EMISSION TOMOGRAPHY DRUGS (Eff. 12-12-2011) Packaging and Labeling § 212.80 What are the...

22 CFR 1203.735-212 - Wearing of uniforms.

Science.gov (United States)

2010-04-01

... RESPONSIBILITIES AND CONDUCT Ethical and Other Conduct and Responsibilities of Employees § 1203.735-212 Wearing of....2b prohibits the purchase from Agency funds of uniforms or any item of personal wearing apparel other...

Upregulation of MiR-212 Inhibits Migration and Tumorigenicity and Inactivates Wnt/β-Catenin Signaling in Human Hepatocellular Carcinoma.

Science.gov (United States)

Jia, Pengbo; Wei, Guangbing; Zhou, Cancan; Gao, Qi; Wu, Yunhua; Sun, Xuejun; Li, Xuqi

2018-01-01

MicroRNAs are involved in hepatocellular carcinoma metastasis, a principal cause of hepatocellular carcinoma-related death in patients worldwide. MiR-212 is a microRNA that has been identified in several types of cancers and is postulated to influence cell signaling and subsequent malignant pathogenesis. Despite emerging reports suggesting that miR-212 plays a significant role in the onset, progression, and migration of these types of malignant tumors, its involvement in the development of hepatocellular carcinoma has not been fully elucidated. Quantitative reverse transcription polymerase chain reaction, wound healing, transwell migration and invasion assays, Western blotting, and xenograft tumor growth models were performed to test the expression levels and functions of miR-212 in hepatocellular carcinoma. Luciferase reporter assay, quantitative reverse transcription polymerase chain reaction, Western blotting, and immunohistochemistry were used to identify and verify the target of miR-212. In this study, we identify significant repression of miR-212 in hepatocellular carcinoma and demonstrate that overexpression of miR-212 inhibits the migration of hepatocellular carcinoma cells in vitro and in vivo. Furthermore, we identify forkhead box M1, whose expression is inversely related to that of miR-212, as a direct target of miR-212. Additionally, reexpression of forkhead box M1 rescues the miR-212-mediated inhibition of cell migration. We observed that inhibition of miR-212 activates forkhead box M1 but inhibits the Wnt/β-catenin pathway by suppressing Wnt, LEF-1, c-Myc, and nuclear β-catenin. Finally, in vivo studies confirmed the inhibitory effect of miR-212 on hepatocellular carcinoma growth. Our present findings indicate that miR-212 is a potential prognostic biomarker of hepatocellular carcinoma and that the miR-212/forkhead box M1 regulatory axis may represent a new therapeutic objective for hepatocellular carcinoma treatment.

Low collectivity of the first 2+ states of 212,210Po

Science.gov (United States)

Kocheva, D.; Rainovski, G.; Jolie, J.; Pietralla, N.; Blazhev, A.; Astier, A.; Altenkirch, R.; Bast, M.; Beckers, M.; Ansari, S.; Braunroth, Th.; Cappellazzo, M.; Cortés, M. L.; Dewald, A.; Diel, F.; Djongolov, M.; Fransen, C.; Gladnishki, K.; Goldkuhle, A.; Hennig, A.; Karayonchev, V.; Keatings, J. M.; Kluge, E.; Kröll, Th.; Litzinger, J.; Moschner, K.; Müller-Gatermann, C.; Petkov, P.; Rudigier, M.; Scheck, M.; Spagnoletti, P.; Scholz, Ph.; Schmidt, T.; Spieker, M.; Stahl, C.; Stegmann, R.; Stolz, A.; Vogt, A.; Stoyanova, M.; Thöle, P.; Warr, N.; Werner, V.; Witt, W.; Wölk, D.; Zamora, J. C.; Zell, K. O.; Van Isacker, P.; Ponomarev, V. Yu.

2018-05-01

The lifetimes of the first 2+ excited states of 212,210Po were measured in two transfer reactions 208Pb(12C,8Be)212Po and 208Pb(12C,10Be)210Po by the Recoil Distance Doppler Shift (RDDS) method and by the Doppler Shift Attenuation method (DSAM), respectively. The derived absolute B(E2) values of 2.6(3) W.u. for 212Po and 1.83(28) W.u. for 210Po indicate low collectivity. It is shown that the properties of the yrast {2}1+, {4}1+, {6}1+ and {8}1+ states in both nuclei cannot be described consistently in the framework of nuclear shell models. It is also demonstrated in the case of 210Po that Quasi-particle Phonon Model (QPM) calculations cannot overcome this problem thus indicating the existence of a peculiarity which is neglected in both theoretical approaches.

A 212Pb generator for model experiments on aqueous chemistry of element 114

International Nuclear Information System (INIS)

Szeglowski, Z.; Kubica, B.; Tuteja-Krysa, M.; Godunowa, H.; Misiak, R.; Stobinski, M.

2005-01-01

Describes the device for continuous preparation of the 212 Pb, 212 Bi, 208 Tl isotopes from natural thorium is described. The generator operates in on-line mode. The simplicity of gamma activity measurements of obtained isotopes makes these tracers very convenient in both chemical researches of transactinides and nuclear medicine. (author)

27 CFR 24.212 - High fermentation wine.

Science.gov (United States)

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false High fermentation wine. 24..., DEPARTMENT OF THE TREASURY LIQUORS WINE Production of Other Than Standard Wine § 24.212 High fermentation wine. High fermentation wine is wine made with the addition of sugar within the limitations prescribed...

75 FR 55272 - Safety Zone; Mississippi River, Mile 212.0 to 214.5

Science.gov (United States)

2010-09-10

...-AA00 Safety Zone; Mississippi River, Mile 212.0 to 214.5 AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone for waters of the Upper Mississippi River, Mile 212.0 to 214.5, extending West of Portage Island to the right descending bank of the...

8 CFR 1212.4 - Applications for the exercise of discretion under section 212(d)(1) and 212(d)(3).

Science.gov (United States)

2010-01-01

... the benefits of section 212(d)(3)(A) of the Act be accorded an alien, neither an application nor fee... which the alien intends to make; and (vi) The justification for exercising the authority contained in... application for admission at POEs in the United States is valid; (vii) The justification for exercising the...

miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

International Nuclear Information System (INIS)

Park, Jong-Kook; Henry, Jon C.; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R.; Postier, Russell G.; Brackett, Daniel J.; Schmittgen, Thomas D.

2011-01-01

Research highlights: → The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. → miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. → miR-132 and miR-212 expression is increased by a β2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G 2 /M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the β2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The β2 adrenergic pathway may play an important role in this novel mechanism.

7 CFR 2.12 - Secretary and general officers not precluded from exercising delegated powers.

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2010-01-01

... exercising delegated powers. 2.12 Section 2.12 Agriculture Office of the Secretary of Agriculture DELEGATIONS... from exercising delegated powers. No delegation of authority by the Secretary or a general officer contained in this part shall preclude the Secretary or general officer from exercising any of the authority...

48 CFR 212.503 - Applicability of certain laws to executive agency contracts for the acquisition of commercial items.

Science.gov (United States)

2010-10-01

... laws to executive agency contracts for the acquisition of commercial items. 212.503 Section 212.503... ACQUISITION PLANNING ACQUISITION OF COMMERCIAL ITEMS Applicability of Certain Laws to the Acquisition of Commercial Items 212.503 Applicability of certain laws to executive agency contracts for the acquisition of...

48 CFR 52.212-2 - Evaluation-Commercial Items.

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2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Evaluation-Commercial....212-2 Evaluation—Commercial Items. As prescribed in 12.301(c), the Contracting Officer may insert a provision substantially as follows: Evaluation—Commercial Items (JAN 1999) (a) The Government will award a...

31 CFR 31.212 - Personal conflicts of interest.

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2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Personal conflicts of interest. 31... RELIEF PROGRAM Conflicts of Interest § 31.212 Personal conflicts of interest. (a) Retained entity's... arrangement and key individuals have no personal conflicts of interest unless mitigation measures have...

Metallothionein bioconjugates as delivery vehicles for bismuth-212 alpha particle therapy

International Nuclear Information System (INIS)

Macklis, R.M.; Morris, C.; Humm, J.; Hines, J.; Atcher, R.

1991-01-01

Metallothioneins (MTHs) are small cysteine-rich polypeptides that binds cationic metals at physiologic pH ranges through noncovalent -SH ligand interactions. Some leucine-rich renal MTHs have a particular avidity for bismuth. The authors have examined the ability of MTHs to selectively incorporate Bi-212, a short-lived high-energy alpha particle emitter currently under exploration as a potential therapeutic radiolabel for use in molecularly targeted cancer therapy. They find that under physiologic conditions, MTH will selectively incorporate Bi-212 after incubation with an equilibrium mixture of its upstream and downstream parents. The MTH moieties may be linked to tumor-binding macromolecules such as antibodies via thiolation reactions using SPDP, and the resultant Bismuth-avid molecules may be used either as primary delivery vehicles for the Bi-212 or as part of a 2-step release-and-catch isotope localization system in which the MTH-antibody conjugate is pre-localized at the tumor site and the radiometal is then administered and chelated in situ. They present the chemistry, dosimetry and potential clinical applications of this system

8 CFR 212.6 - Border crossing identification cards.

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2010-01-01

... Section 212.6 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS DOCUMENTARY... valid on or after October 1, 2002, the non-biometric border crossing card portion of the document is not... contain a machine-readable biometric identifier, may be admitted on the basis of the nonimmigrant visa...

48 CFR 53.212 - Acquisition of commercial items.

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2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Acquisition of commercial... (CONTINUED) CLAUSES AND FORMS FORMS Prescription of Forms 53.212 Acquisition of commercial items. SF 1449 (Rev. 3/2005), Solicitation/Contract/Order for Commercial Items. SF 1449 is prescribed for use in...

19 CFR 212.06 - Allowable fees and expenses.

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2010-04-01

... Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT General Provisions § 212.06 Allowable fees and...) If the attorney, agent or expert witness is in private practice, his or her customary fee for similar...

miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

Energy Technology Data Exchange (ETDEWEB)

Park, Jong-Kook [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Henry, Jon C. [Department of Surgery, Ohio State University, Columbus, OH 43210 (United States); Jiang, Jinmai [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Esau, Christine [Regulus Therapeutics, Carlsbad, CA (United States); Gusev, Yuriy [Lombardi Cancer Center, Georgetown University, Washington, DC (United States); Lerner, Megan R. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Postier, Russell G. [Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States); Brackett, Daniel J. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States)

2011-03-25

Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

Tribological properties of PM212: A high-temperature, self-lubricating, powder metallurgy composite

Science.gov (United States)

Dellacorte, Christopher; Sliney, Harold E.

1989-01-01

This paper describes a research program to develop and evaluate a new high temperature, self-lubricating powder metallurgy composite, PM212. PM212 has the same composition as the plasma-sprayed coating, PS212, which contains 70 wt percent metal-bonded chromium carbide, 15 wt percent silver and 15 wt percent barium fluoride/calcium fluoride eutectic. The carbide acts as a wear resistant matrix and the silver and fluorides act as low and high temperature lubricants, respectively. The material is prepared by sequential cold press, cold isostatic pressing and sintering techniques. In this study, hemispherically tipped wear pins of PM212 were prepared and slid against superalloy disks at temperatures from 25 to 850 C in air in a pin-on-disk tribometer. Friction coefficients range from 0.29 to 0.38 and the wear of both the composite pins and superalloy disks was moderate to low in the 10(exp -5) to 10(exp -6) cubic mm/N-m range. Preliminary tests indicate that the material has a compressive strength of at least 130 MPa over the entire temperature range of 25 to 900 C. This material has promise for use as seal inserts, bushings, small inside diameter parts and other applications where plasma-sprayed coatings are impractical or too costly.

Tribological properties of PM212 - A high temperature, self-lubricating, powder metallurgy composite

Science.gov (United States)

Dellacorte, Christopher; Sliney, Harold E.

1990-01-01

This paper describes a research program to develop and evaluate a new high temperature, self-lubricating powder metallurgy composite, PM212. PM212 has the same composition as the plasma-sprayed coating, PS212, which contains 70 wt percent metal-bonded chromium carbide, 15 wt percent silver and 15 wt percent barium fluoride/calcium fluoride eutectic. The carbide acts as a wear resistant matrix and the silver and fluorides act as low and high temperature lubricants, respectively. The material is prepared by sequential cold press, cold isostatic pressing and sintering techniques. In this study, hemispherically tipped wear pins of PM212 were prepared and slid against superalloy disks at temperatures from 25 to 850 C in air in a pin-on-disk tribometer. Friction coefficients range from 0.29 to 0.38 and the wear of both the composite pins and superalloy disks was moderate to low in the 10(exp -5) to 10(exp -6) cubic mm/N-m range. Preliminary tests indicate that the material has a compressive strength of at least 130 MPa over the entire temperature range of 25 to 900 C. This material has promise for use as seal inserts, bushings, small inside diameter parts and other applications where plasma-sprayed coatings are impractical or too costly.

7 CFR 58.212 - Hopper or dump room.

Science.gov (United States)

2010-01-01

....212 Hopper or dump room. A separate room shall be provided for the transfer of bulk dry dairy products to the hoppers and conveyors which lead to the fillers. This room shall meet the same requirements... transfer of dry dairy products from portable bulk bins will be accepted if gasketed surfaces or direct...

14 CFR 212.8 - Protection of customers' payments.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Protection of customers' payments. 212.8... customers' payments. (a) Except as provided in paragraph (c) of this section, no certificated air carrier or... payable in advance by customers for the subject charter trips shall be accepted by the carrier. (e) The...

Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties

Directory of Open Access Journals (Sweden)

Loh Teng-Hern Tan

2017-05-01

Full Text Available Reactive oxygen species and other radicals potentially cause oxidative damage to proteins, lipids, and DNA which may ultimately lead to various complications including mutations, carcinogenesis, neurodegeneration, cardiovascular disease, aging, and inflammatory disease. Recent reports demonstrate that Streptomyces bacteria produce metabolites with potent antioxidant activity that may be developed into therapeutic drugs to combat oxidative stress. This study shows that Streptomyces sp. MUM212 which was isolated from mangrove soil in Kuala Selangor, Malaysia, could be a potential source of antioxidants. Strain MUM212 was characterized and determined as belonging to the genus Streptomyces using 16S rRNA gene phylogenetic analysis. The MUM212 extract demonstrated significant antioxidant activity through DPPH, ABTS and superoxide radical scavenging assays and also metal-chelating activity of 22.03 ± 3.01%, 61.52 ± 3.13%, 37.47 ± 1.79%, and 41.98 ± 0.73% at 4 mg/mL, respectively. Moreover, MUM212 extract was demonstrated to inhibit lipid peroxidation up to 16.72 ± 2.64% at 4 mg/mL and restore survival of Vero cells from H2O2-induced oxidative damages. The antioxidant activities from the MUM212 extract correlated well with its total phenolic contents; and this in turn was in keeping with the gas chromatography–mass spectrometry analysis which revealed the presence of phenolic compounds that could be responsible for the antioxidant properties of the extract. Other chemical constituents detected included hydrocarbons, alcohols and cyclic dipeptides which may have contributed to the overall antioxidant capacity of MUM212 extract. As a whole, strain MUM212 seems to have potential as a promising source of novel molecules for future development of antioxidative therapeutic agents against oxidative stress-related diseases.

Negative Ion Source Development and Photodetachment Studies at ISOLDE

CERN Document Server

AUTHOR|(CDS)2254068; Hanstorp, Dag; Rothe, Sebastian

Astatine is one of the rarest elements on earth. The small amount of existing astatine is either created in decay chains of heavier elements or artificially. One of its longer lived isotopes, 211At, is of interest for targeted alpha therapy, a method of treating cancer by using the alpha decay of radioactive elements directly at the location of a tumor. However, its chemical properties are yet to be determined due to the short life time of astatine. A milestone towards the determination of the electronegativity of astatine was the measurement of its ionization potential (IP) at CERN-ISOLDE. However, its electron affinity (EA, the binding energy of the additional electron in a negative ion), is still to be measured. In order to determine the EA of radioisotopes by laser photodetachment spectroscopy, the Gothenburg ANion Detector for Affinity measurements by Laser Photodetachment (GANDALPH) has been built in recent years. As a proof-of-principle, the EA of the 128I negative ion, produced at the CERN-ISOLDE rad...

47 CFR 25.212 - Narrowband analog transmissions, digital transmissions, and video transmissions in the GSO Fixed...

Science.gov (United States)

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Narrowband analog transmissions, digital transmissions, and video transmissions in the GSO Fixed-Satellite Service. 25.212 Section 25.212 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES SATELLITE COMMUNICATIONS...

Spectroscopy of high spin states in sup(211,212,213)Fr

International Nuclear Information System (INIS)

Byrne, A.P.; Dracoulis, G.D.; Fahlander, C.; Hubel, H.; Poletti, A.R.; Stuchbery, A.E.; Gerl, J.; Davie, R.F.; Poletti, S.J.

1985-08-01

The level structures of 211 Fr, 212 Fr and 213 Fr have been observed to high spins, approx. 28(h/2π) (and excitation energies approx. 8 MeV) using a variety of gamma-ray spectroscopic techniques. The structure of these nuclides is discussed in terms of couplings of single particle states through empirical shell model calculations. Good agreement with experiment is obtained. In 212 Fr and 213 Fr core-excited configurations are required to explain the properties of the highest states. A number of long lived states were observed in each nucleus some of which decay by by enhanced E3 transitions. The E3 transition strengths are discussed

10 CFR 1021.212 - Research, development, demonstration, and testing.

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2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Research, development, demonstration, and testing. 1021... ACT IMPLEMENTING PROCEDURES DOE Decisionmaking § 1021.212 Research, development, demonstration, and testing. (a) This section applies to the adoption and application of programs that involve research...

5 CFR 9901.212 - Pay schedules and pay bands.

Science.gov (United States)

2010-01-01

... Section 9901.212 Administrative Personnel DEPARTMENT OF DEFENSE HUMAN RESOURCES MANAGEMENT AND LABOR RELATIONS SYSTEMS (DEPARTMENT OF DEFENSE-OFFICE OF PERSONNEL MANAGEMENT) DEPARTMENT OF DEFENSE NATIONAL... Secretary may establish one or more pay schedules within each career group. (b) Each pay schedule may...

μ Opioid Receptor Expression after Morphine Administration Is Regulated by miR-212/132 Cluster.

Directory of Open Access Journals (Sweden)

Adrian Garcia-Concejo

Full Text Available Since their discovery, miRNAs have emerged as a promising therapeutical approach in the treatment of several diseases, as demonstrated by miR-212 and its relation to addiction. Here we prove that the miR-212/132 cluster can be regulated by morphine, through the activation of mu opioid receptor (Oprm1. The molecular pathways triggered after morphine administration also induce changes in the levels of expression of oprm1. In addition, miR-212/132 cluster is actively repressing the expression of mu opioid receptor by targeting a sequence in the 3' UTR of its mRNA. These findings suggest that this cluster is closely related to opioid signaling, and function as a post-transcriptional regulator, modulating morphine response in a dose dependent manner. The regulation of miR-212/132 cluster expression is mediated by MAP kinase pathway, CaMKII-CaMKIV and PKA, through the phosphorylation of CREB. Moreover, the regulation of both oprm1 and of the cluster promoter is mediated by MeCP2, acting as a transcriptional repressor on methylated DNA after prolonged morphine administration. This mechanism explains the molecular signaling triggered by morphine as well as the regulation of the expression of the mu opioid receptor mediated by morphine and the implication of miR-212/132 in these processes.

21 CFR 212.110 - How must I maintain records of my production of PET drugs?

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 4 2010-04-01 2010-04-01 false How must I maintain records of my production of PET drugs? 212.110 Section 212.110 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... those not stored at the inspected establishment, must be legible, stored to prevent deterioration or...

Half-life of 214Po and 212Po measured with CTF at LNGS

International Nuclear Information System (INIS)

Bellini, G.; Benziger, J.; Bick, D.

2013-01-01

Polonium isotopes 214 Po and 212 Po are part of the 238 U and 232 Th decay chains, respectively. There exist only a few measurements of these two mean lifetimes with precision better than one or two percent. Since we have been studying decay spectra of 214 Bi and 212 Bi with the purpose of experimentally constraining anti-neutrino spectral shape important for geoneutrino studies, we have a large statistics of decays of 214 Po and 212 Po collected with the Counting Test Facility (CTF), which was operational in the underground I.N.F.N. Gran Sasso National Laboratory. The apparatus consisted of an external cylindrical water tank (diameter ∼ 11 m, high ∼ 10 m; ∼ 1000 tons of water) serving as passive shielding for 4.8 m 3 of liquid organic scintillator contained in an inner spherical vessel with a diameter of ∼ 2 m. The inner vessel was realized with a nylon membrane (∼ 500 ?m thick), with excellent optical clarity, which allowed the effective transmission of the scintillation light to the 100 phototubes (PMTs) forming the optical read-out, anchored on a 7 m diameter support structure inside the water tank. The high purity and low background in CTF allows a favourable signal to background ratio for these measurements. More specifically the ratio of signal to background of the present measurements is more than three orders of magnitude larger than the best existing measurements. We have studied the decays of 214 Po into 210 Pb and of 212 Po into 208 Pb tagged by the coincidence with the previously decays from 214 Bi and 212 Bi by using 222 Rn, 232 Th and 220 Rn sources sealed inside quartz vials and inserted in the CTF

The 2ν2 bands of H212CO and H213CO by high-resolution FTIR spectroscopy

Science.gov (United States)

Tan, T. L.; A'dawiah, Rabia'tul; Ng, L. L.

2017-10-01

The Fourier transform infrared (FTIR) absorption spectra of the 2ν2 overtone bands of formaldehyde H212CO and its isotopologue H213CO were recorded at an unapodized resolution of 0.0063 cm-1 in the 3300-3540 cm-1 region. Upper state (v2 = 2) rovibrational up to two sextic centrifugal distortion constants were accurately determined for both H212CO and H213CO. A total of 533 unperturbed infrared transitions of H212CO and 466 unperturbed infrared transitions of H212CO were assigned and fitted with rms deviations of 0.0012 cm-1 and 0.00084 cm-1 respectively using Watson's A-reduced Hamiltonian in the Ir representation. Analysis of new transitions for H212CO measured in this work yielded upper state constants with greater accuracy than previously reported. The infrared transitions of the 2ν2 band of H213CO were measured for the first time. The band center of the A-type 2ν2 band of H212CO was found to be 3471.71403 ± 0.00012 cm-1 and that of H213CO was 3396.628983 ± 0.000083 cm-1. Furthermore, the newly assigned high-resolution infrared lines of the 2ν2 bands in the 3300-3540 cm-1 region can be useful in detecting the H212CO and H213CO molecules in this IR region.

9 CFR 113.212 - Bursal Disease Vaccine, Killed Virus.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bursal Disease Vaccine, Killed Virus..., DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS Killed Virus Vaccines § 113.212 Bursal Disease Vaccine, Killed Virus. Bursal Disease Vaccine...

Alpha particle emitters in medicine

International Nuclear Information System (INIS)

Fisher, D.R.

1989-09-01

Radiation-induced cancer of bone, liver and lung has been a prominent harmful side-effect of medical applications of alpha emitters. In recent years, however, the potential use of antibodies labeled with alpha emitting radionuclides against cancer has seemed promising because alpha particles are highly effective in cell killing. High dose rates at high LET, effectiveness under hypoxic conditions, and minimal expectancy of repair are additional advantages of alpha emitters over antibodies labeled with beta emitting radionuclides for cancer therapy. Cyclotron-produced astatine-211 ( 211 At) and natural bismuth-212 ( 212 Bi) have been proposed and are under extensive study in the United States and Europe. Radium-223 ( 223 Ra) also has favorable properties as a potential alpha emitting label, including a short-lived daughter chain with four alpha emissions. The radiation dosimetry of internal alpha emitters is complex due to nonuniformly distributed sources, short particle tracks, and high relative specific ionization. The variations in dose at the cellular level may be extreme. Alpha-particle radiation dosimetry, therefore, must involve analysis of statistical energy deposition probabilities for cellular level targets. It must also account fully for nonuniform distributions of sources in tissues, source-target geometries, and particle-track physics. 18 refs., 4 figs

miR-132/212 knockout mice reveal roles for these miRNAs in regulating cortical synaptic transmission and plasticity.

Directory of Open Access Journals (Sweden)

Judit Remenyi

Full Text Available miR-132 and miR-212 are two closely related miRNAs encoded in the same intron of a small non-coding gene, which have been suggested to play roles in both immune and neuronal function. We describe here the generation and initial characterisation of a miR-132/212 double knockout mouse. These mice were viable and fertile with no overt adverse phenotype. Analysis of innate immune responses, including TLR-induced cytokine production and IFNβ induction in response to viral infection of primary fibroblasts did not reveal any phenotype in the knockouts. In contrast, the loss of miR-132 and miR-212, while not overtly affecting neuronal morphology, did affect synaptic function. In both hippocampal and neocortical slices miR-132/212 knockout reduced basal synaptic transmission, without affecting paired-pulse facilitation. Hippocampal long-term potentiation (LTP induced by tetanic stimulation was not affected by miR-132/212 deletion, whilst theta burst LTP was enhanced. In contrast, neocortical theta burst-induced LTP was inhibited by loss of miR-132/212. Together these results indicate that miR-132 and/or miR-212 play a significant role in synaptic function, possibly by regulating the number of postsynaptic AMPA receptors under basal conditions and during activity-dependent synaptic plasticity.

Measurements of 212Pb concentration in the ground-level air in Beijing area and assessment of ionizing radiation exposure of the population

International Nuclear Information System (INIS)

Lin Lianqing; Wen Huifen; Zhou Yuanwen

1994-01-01

The paper describes the method of measuring 212 Pb concentration in the ground-level air with a gamma spectrometer and the results of the measurements of 212 Pb concentrations during February 1988-January 1989. The results showed that the average and standard deviation are 0.54 and 0.40 Bq·m -3 ; the distribution of 212 Pb concentrations in air was logarithmic normal distribution, geometric average and geometric standard deviation are 0.44 Bq·m -3 and 2.0. The 212 Pb concentration in rain season is the lowest (0.42 Bq·m -3 ) while in heating season is the highest (0.66 Bq· -3 ). In period of a day, 212 Pb concentration in the morning 0-4 o'clock is the highest and in the afternoon 12-18 o'clock is the lowest, 212 Pb(max)/ 212 Pb(min) = 4.8

The Effect of Dorsal Hippocampal α2-Adrenegic Receptors on WIN55,212-2 State-Dependent Memory of Passive Avoidance

Directory of Open Access Journals (Sweden)

Zarrindast M.R.

2010-09-01

Full Text Available Background and Objectives: Cannabinoids are a class of psychoactive compounds that produce a wide array of effects in a large number of species. In the present study, the effects of bilateral intra-CA1 injections of an α2-adrenergic receptor agents, on WIN55,212-2 state-dependent learning were examined in adult male Wistar rats. Methods: The animals were bilaterally implanted with chronic cannulae in the CA1 regions of the dorsal hippocampus, trained in a step-down type inhibitory avoidance task, and tested 24h after training to measure step-down latency.Results: Post-training intra-CA1 injection of WIN55,212-2 (0.25 and 0.5µg/rat induced impairment of memory retention. Amnesia produced by post-training WIN55,212-2 (0.5µg/rat was reversed by pre-test administration of the same dose of WIN55,212-2 that is due to a state-dependent effect. Pre-test intra-CA1 injection of clonidine (0.5 and 0.75µg/rat, intra-CA1 improved post-training WIN55,212-2 (0.5µg/rat, intra-CA1-induced retrieval impairment, while pre-test intra-CA1 injection of yohimbine (1µg/rat, intra-CA1 2min before the administration of WIN55,212-2 (0.5µg/rat, intra-CA1 inhibited WIN55,212-2 state-dependent memory. Conclusion: These results suggest that α2-adrenergic receptors of the dorsal hippocampal CA1 regions may play an important role in Win55,212-2-induced amnesia and WIN55,212-2 state-dependent memory.

8 CFR 212.7 - Waiver of certain grounds of inadmissibility.

Science.gov (United States)

2010-01-01

... alien's intelligence. For an alien with a past history of mental illness, the medical report shall also... § 212.7, see the List of CFR Sections Affected, which appears in the Finding Aids section of the printed...

The microRNA-132/212 family fine-tunes multiple targets in Angiotensin II signalling in cardiac fibroblasts

DEFF Research Database (Denmark)

Eskildsen, Tilde V; Schneider, Mikael; Sandberg, Maria B

2015-01-01

INTRODUCTION: MicroRNAs (miRNAs) are emerging as key regulators of cardiovascular development and disease; however, the cardiac miRNA target molecules are not well understood. We and others have described the Angiotensin II (AngII)-induced miR-132/212 family as novel regulators of cardiovascular...... in silico and in vitro experiments to identify miR-132/212 molecular targets in primary rat cardiac fibroblasts. RESULTS: MiR-132/212 overexpression increased fibroblast cell size and mRNA arrays detected several hundred genes that were differentially expressed, including a wide panel of receptors...... pathways that fine-tuned by miR-132/212, suggesting a role for this miRNA family as master signalling switches in cardiac fibroblasts. Our data underscore the potential for miRNA tools to manipulate a large array of molecules and thereby control biological function....

36 CFR 212.57 - Monitoring of effects of motor vehicle use on designated roads and trails and in designated areas.

Science.gov (United States)

2010-07-01

... motor vehicle use on designated roads and trails and in designated areas. 212.57 Section 212.57 Parks... Roads, Trails, and Areas for Motor Vehicle Use § 212.57 Monitoring of effects of motor vehicle use on designated roads and trails and in designated areas. For each administrative unit of the National Forest...

Development and therapeutic application of internally emitting radiopharmaceuticals

International Nuclear Information System (INIS)

Adelstein, S.J.; Bloomer, W.D.

1980-01-01

This project is concerned with developing the potential of alpha-emitting radionuclides as agents for radiotherapy. Among the available α-emitters, astatine-211 appears most promising for testing the efficacy of α-emitters for therapeutic applications because: (1) it has some chemical similarities to iodine, an element that can readily be incorporated into numerous proteins and peptides; (2) it has a half life that is long enough to permit chemical manipulation yet short enough to minimize destruction of healthy cells; and (3) α-emission is associated with 100% of its decays. If appropriate biological carriers can be labeled with an alpha emitter such as 211 At, they could be of great utility in several areas of therapeutic medicine where elimination of specific cell populations is desired. While previous attempts to astatinate proteins using standard iodination techniques have been unsuccessful, effective labeling of proteins with astatine by first synthesizing an aryl astatide and then coupling this compound to the protein via an acylation has been achieved. Undergoing current investigation are several different aryl astatide-followed-by-acylation approaches including an astatinated Bolton-Hunter type reagent using concanavalin A (ConA) and melanocyte stimulating hormone (MSH) as model compounds

Postoperative radiotherapy for stage I/II seminoma: results for 212 patients

International Nuclear Information System (INIS)

Bauman, Glenn S.; Venkatesan, Varagur M.; Ago, C. Tetteh; Radwan, John S.; Dar, A. Rashid; Winquist, Eric W.

1998-01-01

Purpose: A retrospective review of patients with Stage I and II seminoma treated at a regional cancer center was performed to assess the long term efficacy and toxicity associated with post operative radiotherapy. Methods and Materials: Between 1950 and 1995, 212 patients seen at the London Regional Cancer Centre received adjuvant radiotherapy following orchiectomy for Stage I (169) and II (43) seminoma. Median follow-up for the group was 7.5 years. Results: Progression free, cause specific, and overall survival were 95%, 98%, and 95% at 5 years, and 94%, 98%, and 94% at 10 years respectively. An increased risk of failure was noted among patients with bulky Stage II disease. No other prognostic factors for relapse were identified. Late toxicity was uncommon with only 12/212 (6%) developing any late GI toxicity potentially attributable to radiotherapy. The incidence of second malignancies (excluding second testicular tumors) was 6/212 (actuarial:1%, 1%, 6% at 5,10,15 years respectively). There was a trend toward increased acute complications for patients treated with larger volumes of radiation. No prognostic factors associated with increased risk of late toxicity or second malignancy were identified, likely a consequence of the small number of these events. Conclusion: Survival and toxicity were comparable to that reported in the literature. Post-operative radiotherapy remains a safe and efficacious adjuvant treatment for Stage I and early Stage II seminoma

A Prototype {sup 212}Pb Medical Dose Calibrator for Alpha Radioimmunotherapy

Energy Technology Data Exchange (ETDEWEB)

Mueller, W.F.; Patil, A.; Russ, W.R.; Newman, J. [CANBERRA Industries, Inc. (United States); Torgue, J. [AREVA Med (United States)

2015-07-01

AREVA Med, an AREVA group subsidiary, is developing innovative cancer-fighting therapies involving the use of {sup 212}Pb for alpha radioimmunotherapy. Canberra Industries, the nuclear measurement subsidiary of AREVA, has been working with AREVA Med to develop a prototype measurement system to assay syringes containing a {sup 212}Pb solution following production by an elution system. The relative fraction of emitted radiation from the source associated directly with the {sup 212}Pb remains dynamic for approximately 6 hours after the parent is chemically purified. A significant challenge for this measurement application is that the short half-life of the parent nuclide requires assay prior to reaching equilibrium with progeny nuclides. A gross counting detector was developed to minimize system costs and meet the large dynamic range of source activities. Prior to equilibrium, a gross counting system must include the period since the {sup 212}Pb was pure to calculate the count rate attributable to the parent rather than the progeny. The dynamic state is determined by solving the set of differential equations, or Bateman equations, describing the source decay behavior while also applying the component measurement efficiencies for each nuclide. The efficiencies were initially estimated using mathematical modeling (MCNP) but were then benchmarked with source measurements. The goal accuracy of the system was required to be within 5%. Independent measurements of the source using a high resolution spectroscopic detector have shown good agreement with the prototype system results. The prototype design was driven by cost, compactness and simplicity. The detector development costs were minimized by using existing electronics and firmware with a Geiger-Mueller tube derived from Canberra's EcoGamma environmental monitoring product. The acquisition electronics, communications and interface were controlled using Python with the EcoGamma software development kit on a Raspberry

Gene expression profiling upon 212Pb-TCMC-trastuzumab treatment in the LS-174T i.p. xenograft model

International Nuclear Information System (INIS)

Yong, Kwon J; Milenic, Diane E; Baidoo, Kwamena E; Kim, Young-Seung; Brechbiel, Martin W

2013-01-01

Recent studies have demonstrated that therapy with 212 Pb-TCMC-trastuzumab resulted in (1) induction of apoptosis, (2) G2/M arrest, and (3) blockage of double-strand DNA damage repair in LS-174T i.p. (intraperitoneal) xenografts. To further understand the molecular basis of the cell killing efficacy of 212 Pb-TCMC-trastuzumab, gene expression profiling was performed with LS-174T xenografts 24 h after exposure to 212 Pb-TCMC-trastuzumab. DNA damage response genes (84) were screened using a quantitative real-time polymerase chain reaction array (qRT-PCR array). Differentially regulated genes were identified following exposure to 212 Pb-TCMC-trastuzumab. These included genes involved in apoptosis (ABL, GADD45α, GADD45γ, PCBP4, and p73), cell cycle (ATM, DDIT3, GADD45α, GTSE1, MKK6, PCBP4, and SESN1), and damaged DNA binding (DDB) and repair (ATM and BTG2). The stressful growth arrest conditions provoked by 212 Pb-TCMC-trastuzumab were found to induce genes involved in apoptosis and cell cycle arrest in the G2/M phase. The expression of genes involved in DDB and single-strand DNA breaks was also enhanced by 212 Pb-TCMC-trastuzumab while no modulation of genes involved in double-strand break repair was apparent. Furthermore, the p73/GADD45 signaling pathway mediated by p38 kinase signaling may be involved in the cellular response, as evidenced by the enhanced expression of genes and proteins of this pathway. These results further support the previously described cell killing mechanism by 212 Pb-TCMC-trastuzumab in the same LS-174T i.p. xenograft. Insight into these mechanisms could lead to improved strategies for rational application of radioimmunotherapy using α-particle emitters. The apoptotic response and associated gene modulations have not been clearly defined following exposure of cells to α-particle radioimmunotherapy (RIT). Gene expression profiling was performed with LS-174T i.p. (intraperitoneal) xenografts after exposure to 212 Pb

Free radical detection by means of {sup 2}12Pb mirrors; Deteccion de radicales libres mediante espejos de Pb{sup 2}12

Energy Technology Data Exchange (ETDEWEB)

Molera, M J; Gamboa, J M; Garcia Guijarro, E

1961-07-01

Radicals have been detected at low temperatures (550-700 degree centigrade) in the thermal decomposition of dimethyl and methyl-ethyl-ketone by the Paneth method. the sensitivity of the method has been increased by the use of radioactive mirrors of PB{sup 2}12. They have proved to detect one radical among 2.10{sup 6} molecules. (Author) 18 refs.

23 CFR 973.212 - Indian lands safety management system (SMS).

Science.gov (United States)

2010-04-01

... implementation of public information and education activities on safety needs, programs, and countermeasures... 23 Highways 1 2010-04-01 2010-04-01 false Indian lands safety management system (SMS). 973.212... HIGHWAYS MANAGEMENT SYSTEMS PERTAINING TO THE BUREAU OF INDIAN AFFAIRS AND THE INDIAN RESERVATION ROADS...

42 CFR 3.212 - Nonidentification of patient safety work product.

Science.gov (United States)

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Nonidentification of patient safety work product. 3... PROVISIONS PATIENT SAFETY ORGANIZATIONS AND PATIENT SAFETY WORK PRODUCT Confidentiality and Privilege Protections of Patient Safety Work Product § 3.212 Nonidentification of patient safety work product. (a...

8 CFR 212.14 - Parole determinations for alien witnesses and informants for whom a law enforcement authority...

Science.gov (United States)

2010-01-01

... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Parole determinations for alien witnesses and informants for whom a law enforcement authority (âLEAâ) will request S classification. 212.14 Section 212.14 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS DOCUMENTARY REQUIREMENTS: NONIMMIGRANTS; WAIVERS; ADMISSIO...

Synthesis, radiolabelling, and evaluation of [11C]PB212 as a radioligand for imaging sigma-1 receptors using PET.

Science.gov (United States)

Spinelli, Francesco; Haider, Ahmed; Toscano, Annamaria; Pati, Maria Laura; Keller, Claudia; Berardi, Francesco; Colabufo, Nicola Antonio; Abate, Carmen; Ametamey, Simon M

2018-01-01

The Sigma-1 receptor (Sig-1R) has been described as a pluripotent modulator of distinct physiological functions and its involvement in various central and peripheral pathological disorders has been demonstrated. However, further investigations are required to understand the complex role of the Sig-1R as a molecular chaperon. A specific PET radioligand would provide a powerful tool in Sig-1R related studies. As part of our efforts to develop a Sig-1R PET radioligand that shows antagonistic properties, we investigated the suitability of 1-(4-(6-methoxynaphthalen-1-yl)butyl)-4-methylpiperidine (designated PB212) for imaging Sig-1R. PB212 is a Sig-1R antagonist and exhibits subnanomolar affinity ( K i = 0.030 nM) towards Sig-1R as well as good to excellent selectivity over Sig-2R. The radiolabelling of [ 11 C]PB212 was accomplished by O-methylation of the phenolic precursor using [ 11 C]MeI. In vitro autoradiography with [ 11 C]PB212 on WT and Sig-1R KO mouse brain tissues revealed high non-specific binding, however using rat spleen tissues from CD1 mice and Wistar rats, high specific binding was observed. The spleen is known to have a high expression of Sig-1R. In vivo PET experiments in Wistar rats also showed high accumulation of [ 11 C]PB212 in the spleen. Injection of Sig-1R binding compounds, haloperidol (1 mg/kg) or fluspidine (1 mg/kg) shortly before [ 11 C]PB212 administration induced a drastic reduction of radiotracer accumulation, confirming the specificity of [ 11 C]PB212 towards Sig-1R in the spleen. The results obtained herein indicate that although [ 11 C]PB212 is not suitable for imaging Sig-1R in the brain, it is a promising candidate for the detection and quantification of Sig-1Rs in the periphery.

30 CFR 250.212 - What information must accompany the EP?

Science.gov (United States)

2010-07-01

... by § 250.218; (g) Oil and hazardous substance spills information required by § 250.219; (h) Alaska... Section 250.212 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND... support facilities information required by § 250.225; (n) Coastal zone management information required by...

Recombinant protein of heptad-repeat HR212, a stable fusion inhibitor with potent anti-HIV action in vitro

International Nuclear Information System (INIS)

Pang, Wei; Wang Ruirui; Yang Liumeng; Liu Changmei; Tien Po; Zheng Yongtang

2008-01-01

HR212, a recombinant protein expressed in Escherichia coli, has been previously reported to inhibit HIV-1 membrane fusion at low nanomolar level. Here we report that HR212 is effective in blocking laboratory strain HIV-1 IIIB entry and replication with EC 50 values of 3.92 ± 0.62 and 6.59 ± 1.74 nM, respectively, and inhibiting infection by clinic isolate HIV-1 KM018 with EC 50 values of 44.44 ± 10.20 nM, as well as suppressing HIV-1-induced cytopathic effect with an EC 50 value of 3.04 ± 1.20 nM. It also inhibited HIV-2 ROD and HIV-2 CBL-20 entry and replication in the μM range. Notably, HR212 was highly effective against T20-resistant strains with EC 50 values ranging from 5.09 to 7.75 nM. Unlike T20, HR212 showed stability sufficient to inhibit syncytia formation in a time-of-addition assay, and was insensitive to proteinase K digestion. These results suggest that HR212 has great potential to be further developed as novel HIV-1 fusion inhibitor for treatment of HIV/AIDS patients, particularly for those infected by T20-resistant variants

Measurements of thorium-B (212Pb) in the outdoor environment and evaluation of equivalent dose

International Nuclear Information System (INIS)

Mohammed, A.; El-Hussein, A.; Ali, A.E.

2000-01-01

The activity size distribution of unattached as well as attached 212 Pb to aerosol particles was measured in the open air of El-Minia City, Egypt. The samples were collected using a wire screen diffusion battery technique and a low pressure cascade impactor. The mean activity median thermodynamic diameter (AMTD) of unattached 212 Pb was determined to be 1.4 nm with a relative mean geometric standard deviation (σ g ) of 1.55. A mean unattached fraction (f p ) of 0.03±0.007 was obtained at a mean aerosol particle concentration of 32x10 3 cm -3 . Sometimes the f p values were less than the detection limit of 0.008. The mean concentration of activity of 212 Pb was found to be 9.6±1.1 mBq m -3 . The mean activity median aerodynamic diameter (AMAD) of the accumulation mode of attached 212 Pb was determined to be 360 nm with a mean (σ g ) of 2.7. The mean value of specific air activity concentration of 212 Pb associated with that mode was determined to be 303±12 mBq m -3 . With a dosimetric model calculation (International Commission on Radiological Protection. Human respiratory tract model for radiological protection. Oxford: Pergamon Press, ICRP Publication 66, 1994) the total deposition fractions as well as total equivalent and effective dose have been evaluated considering the obtained parameters of the activity size distributions. At a total deposition fraction of about 97% for unattached activities the total equivalent and effective doses to the lung were determined to be about 0.18 and 0.02 μSv, respectively, while total equivalent and effective doses of about 0.45 and 0.05 μ Sv, respectively, were determined at a total deposition fraction of about 23% for the attached activities

Mechanical strength and thermophysical properties of PM212: A high temperature self-lubricating powder metallurgy composite

Science.gov (United States)

Edwards, Phillip M.; Sliney, Harold E.; Dellacorte, Christopher; Whittenberger, J. Daniel; Martineau, Robert R.

1990-01-01

A powder metallurgy composite, PM212, composed of metal bonded chromium carbide and solid lubricants is shown to be self-lubricating to a maximum application temperature of 900 C. The high temperature compressive strength, tensile strength, thermal expansion and thermal conductivity data needed to design PM212 sliding contact bearings and seals are reported for sintered and isostatically pressed (HIPed) versions of PM212. Other properties presented are room temperature density, hardness, and elastic modulus. In general, both versions appear to have adequate strength to be considered as sliding contact bearing materials, but the HIPed version, which is fully dense, is much stronger than the sintered version which contains about 20 percent pore volume. The sintered material is less costly to make, but the HIPed version is better where high compressive strength is important.

30 CFR 77.212 - Draw-off tunnel ventilation fans; installation.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Draw-off tunnel ventilation fans; installation... UNDERGROUND COAL MINES Surface Installations § 77.212 Draw-off tunnel ventilation fans; installation. When fans are used to ventilate draw-off tunnels the fans shall be: (a) Installed on the surface; (b...

30 CFR 75.212 - Rehabilitation of areas with unsupported roof.

Science.gov (United States)

2010-07-01

... COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Roof Support § 75.212... or the roof has been removed by mining machines or by blasting— (1) The mine operator shall establish the clean up and support procedures that will be followed; (2) All persons assigned to perform...

36 CFR 212.51 - Designation of roads, trails, and areas.

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Designation of roads, trails... AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle Use § 212.51 Designation of roads, trails, and areas. (a) General. Motor vehicle use on National Forest System roads, on...

Evaluation of the Live Biotherapeutic Product, Asymptomatic Bacteriuria Escherichia coli 2-12, in Healthy Dogs and Dogs with Clinical Recurrent UTI.

Science.gov (United States)

Segev, G; Sykes, J E; Klumpp, D J; Schaeffer, A J; Antaki, E M; Byrne, B A; Yaggie, R E; Westropp, J L

2018-01-01

Antimicrobial resistance is an emerging problem. To investigate the safety and efficacy of a live biotherapeutic product, ASB E. coli 2-12 for UTI treatment. Six healthy research dogs; nine client-owned dogs with recurrent UTI. Prospective noncontrolled clinical trial. For safety data, research dogs were sedated, a urinary catheter was inserted into the bladder; 10 10 CFU/mL of ASB E. coli 2-12 was instilled. Urine was cultured on days 1, 3, and 8 post-instillation and dogs were observed for lower urinary tract signs (LUTS). For client-owned dogs, ASB E. coli 2-12 was instilled similarly and urine cultures analyzed on days 1, 7, and 14 days postinstillation. No LUTS were noted in any of the 6 research dogs after ASB E. coli 2-12 infusion. Pulse field gel electrophoresis (PFGE) studies confirmed the bacterial strains isolated matched that ASB E. coli 2-12 strain. Four of the nine client-owned dogs had complete or nearly complete clinical cures by day 14. Of these four dogs, 3 also had microbiologic cures at day 14; one of these dogs had subclinical bacteriuria (in addition to ASB E. coli 2-12). Three of these four dogs had ASB E. coli 2-12 isolated from their urine at day 14. With the exception of mild, temporary, self-limiting, hyporexia in two dogs on the day of biotherapeutic administration, there were no major adverse effects. These results suggest ASB E. coli 2-12 is safe and should be investigated in a larger controlled study evaluating clinical UTI in dogs. Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.

212Pb-Labeled Antibody 225.28 Targeted to Chondroitin Sulfate Proteoglycan 4 for Triple-Negative Breast Cancer Therapy in Mouse Models

Directory of Open Access Journals (Sweden)

Benjamin B. Kasten

2018-03-01

Full Text Available Triple-negative breast cancer (TNBC is an aggressive subtype of breast cancer with a poor prognosis. There is a clinical need for effective, targeted therapy strategies that destroy both differentiated TNBC cells and TNBC cancer initiating cells (CICs, as the latter are implicated in the metastasis and recurrence of TNBC. Chondroitin sulfate proteoglycan 4 (CSPG4 is overexpressed on differentiated tumor cells and CICs obtained from TNBC patient specimens, suggesting that CSPG4 may be a clinically relevant target for the imaging and therapy of TNBC. The purpose of this study was to determine whether α-particle radioimmunotherapy (RIT targeting TNBC cells using the CSPG4-specific monoclonal antibody (mAb 225.28 as a carrier was effective at eliminating TNBC tumors in preclinical models. To this end, mAb 225.28 labeled with 212Pb (212Pb-225.28 as a source of α-particles for RIT was used for in vitro Scatchard assays and clonogenic survival assays with human TNBC cells (SUM159 and 2LMP grown as adherent cells or non-adherent CIC-enriched mammospheres. Immune-deficient mice bearing orthotopic SUM159 or 2LMP xenografts were injected i.v. with the targeted (225.28 or irrelevant isotype-matched control (F3-C25 mAbs, labeled with 99mTc, 125I, or 212Pb for in vivo imaging, biodistribution, or tumor growth inhibition studies. 212Pb-225.28 bound to adherent SUM159 and 2LMP cells and to CICs from SUM159 and 2LMP mammospheres with a mean affinity of 0.5 nM. Nearly ten times more binding sites per cell were present on SUM159 cells and CICs compared with 2LMP cells. 212Pb-225.28 was six to seven times more effective than 212Pb-F3-C25 at inhibiting SUM159 cell and CIC clonogenic survival (p < 0.05. Radiolabeled mAb 225.28 showed significantly higher uptake than radiolabeled mAb F3-C25 in SUM159 and 2LMP xenografts (p < 0.05, and the uptake of 212Pb-225.28 in TNBC xenografts was correlated with target epitope expression. 212Pb-225.28 caused dose

High-spin yrast isomers in 211Rn and 212Rn with enhanced E3 decays

International Nuclear Information System (INIS)

Dracoulis, G.D.; Byrne, A.P.; Fabricius, B.

1990-01-01

New isomeric states with J π =69/2 + ,τ m = 13 (1) ns in 211 Rn and J π =33 - ,τ m = 7(1) ns in 212 Rn have been identified. They decay by enchanced E3 transitions with strengths of 33(3) and 43(6) single particle units to the known 63/2 - and 30 + isomers 211 Rn and 212 Rn, respectively. The excitation energies and transition strengths agree well with predictions of the multi-particle, octupole-vibration coupled model. 13 refs., 2 tabs., 3 figs

Microwave-induced combustion synthesis and electrical conductivity of Ce1-xGd xO2-1/2x ceramics

International Nuclear Information System (INIS)

Fu, Y.-P.; Chang, Y.-S.; Wen, S.-B.

2006-01-01

Ce 1-x Gd x O 2-1/2x nanopowder were successfully synthesized by microwave-induced combustion process. For the preparation, cerium nitrate, gadolinium nitrate hexahydrate, and urea were used for the microwave-induced combustion process. The process took only 30 min to obtain Ce 1-x Gd x O 2-1/2x powders. The exo-endo temperature, phase identification, and morphology of resultant powders were investigated by TG/DTA, XRD, and SEM. The as-received Ce 1-x Gd x O 2-1/2x powders showed that the average particle size ranged from 18 to 50 nm, crystallite dimension varied from 11 to 20 nm, and the specific surface area was distribution from 16 to 46 m 2 /g. As for Ce 1-x Gd x O 2-1/2x ceramics sintered at 1450 deg. C for 3 h, the bulk density of Ce 1-x Gd x O 2-1/2x ceramics were over 91% of the theoretical density, the maximum electrical conductivity, σ 700deg.C = 0.017 S/cm with minimum activation energy, E a = 0.869 eV was found at Ce 0.80 Gd 0.20 O 1.90 ceramic

Binding of a novel 12-E2-12 gemini surfactant to xanthine oxidase: Analysis involving tensiometric, spectroscopic, microscopic and molecular docking approach

International Nuclear Information System (INIS)

Akram, Mohd; Bhat, Imtiyaz Ahmad; Kabir-ud-Din

2016-01-01

Binding interaction of a synthesized biodegradable gemini surfactant, ethane-1, 2-diyl bis(N, N-dimethyl-N-dodecylammoniumacetoxy) dichloride (12-E2-12), with bovine milk xanthine oxidase (XO) was studied using tensiometry, fluorescence spectroscopy, UV, CD, FT-IR, TEM and molecular docking. Tensiometry revealed lowering in surface tension (γ) and critical micelle concentration (CMC) of 12-E2-12 upon XO combination, suggesting a significant interaction between XO and 12-E2-12 (both in the bulk as well as at interface). Intrinsic fluorescence studies depict that 12-E2-12 quenches XO fluorescence intensity through static mechanism. The magnitude of binding parameters infers substantial and effective binding of 12-E2-12 to (XO). ANS and pyrene fluorescence demonstrate the exposure of aromatic residues (tyrosine/tryptophan) to a non-polar environment. UV, circular dichroism (CD) and FT-IR results delineate change in the secondary structure of the enzyme XO. Microscopic TEM micrographs confirm the disrupture of enzyme structure at higher concentrations of 12-E2-12. Molecular docking results show that 12-E2-12 binds to XO in the vicinity of both hydrophobic and hydrophilic residues, inferring that binding is governed by both hydrophilic and hydrophobic forces. This study may be of significance in biomedical world to further interpret mechanistic treatment modes of diseases like gout and hyperuricemia. Moreover, this study provides deeper biophysical insight into surfactant–protein interactions. - Highlights: • Binding of biodegradable gemini surfactant 12-E2-12 with xanthine oxidase. • Binding induces conformational changes in the latter. • Conformational change can be useful for biomedical and industrial purposes.

Binding of a novel 12-E2-12 gemini surfactant to xanthine oxidase: Analysis involving tensiometric, spectroscopic, microscopic and molecular docking approach

Energy Technology Data Exchange (ETDEWEB)

Akram, Mohd, E-mail: drmohdakram@rediffmail.com; Bhat, Imtiyaz Ahmad; Kabir-ud-Din

2016-02-15

Binding interaction of a synthesized biodegradable gemini surfactant, ethane-1, 2-diyl bis(N, N-dimethyl-N-dodecylammoniumacetoxy) dichloride (12-E2-12), with bovine milk xanthine oxidase (XO) was studied using tensiometry, fluorescence spectroscopy, UV, CD, FT-IR, TEM and molecular docking. Tensiometry revealed lowering in surface tension (γ) and critical micelle concentration (CMC) of 12-E2-12 upon XO combination, suggesting a significant interaction between XO and 12-E2-12 (both in the bulk as well as at interface). Intrinsic fluorescence studies depict that 12-E2-12 quenches XO fluorescence intensity through static mechanism. The magnitude of binding parameters infers substantial and effective binding of 12-E2-12 to (XO). ANS and pyrene fluorescence demonstrate the exposure of aromatic residues (tyrosine/tryptophan) to a non-polar environment. UV, circular dichroism (CD) and FT-IR results delineate change in the secondary structure of the enzyme XO. Microscopic TEM micrographs confirm the disrupture of enzyme structure at higher concentrations of 12-E2-12. Molecular docking results show that 12-E2-12 binds to XO in the vicinity of both hydrophobic and hydrophilic residues, inferring that binding is governed by both hydrophilic and hydrophobic forces. This study may be of significance in biomedical world to further interpret mechanistic treatment modes of diseases like gout and hyperuricemia. Moreover, this study provides deeper biophysical insight into surfactant–protein interactions. - Highlights: • Binding of biodegradable gemini surfactant 12-E2-12 with xanthine oxidase. • Binding induces conformational changes in the latter. • Conformational change can be useful for biomedical and industrial purposes.

41 CFR 301-71.212 - Should we report late payment fees as wages on a Form W-2?

Science.gov (United States)

2010-07-01

... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false Should we report late payment fees as wages on a Form W-2? 301-71.212 Section 301-71.212 Public Contracts and Property... late payment fees as wages on a Form W-2? No, the Internal Revenue Service (IRS) has determined that...

New cytogenetically visible copy number variant in region 8q21.2

Directory of Open Access Journals (Sweden)

Ewers Elisabeth

2011-01-01

Full Text Available Abstract Background Cytogenetically visible unbalanced chromosomal abnormalities (UBCA, reported for >50 euchromatic regions of almost all human autosomes, are comprised of a few megabases of DNA, and carriers are in many cases clinically healthy. It may be speculated, that some of the UBCA may be similar or identical to copy number variants (CNV of the human genome. Results Here we report on a yet unreported cytogenetically visible copy number variant (CNV in the long arm of chromosome 8, region 8q21.2, detected in three unrelated clinically healthy carriers. Conclusion The first description of a cytogenetically visible CNV/UBCA in 8q21.2 shows that banding cytogenetics is far from being outdated. It is a cost efficient, up-to-date method for a single cell specific overview on the whole genome, still prepared to deliver unexpected findings.

22 CFR 212.36 - Denial of request for access to records.

Science.gov (United States)

2010-04-01

... Section 212.36 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Access to Agency... responsible for the denial, and the right of the person to appeal the adverse determination. (b) The denial of... Administrative Services, Bureau for Management, Room 803, SA-2, Agency for International Development, 21st and...

Calibration of carbon analyzer LECO type IR-212

International Nuclear Information System (INIS)

Lilis Windaryati; Pranjono; Galuh Sri Banawa

2013-01-01

Calibration of Carbon Analyzer LECO type IR-212 has been done. The aim of this research is to study the performance of the carbon analyzer LECO type IR-212 for its accuracy assurance. The experiment includes a series of performance adjustment using standard material traceable nationally/internationally. The standard material used for the calibration is standard carbon manufactured by LECO, which refers to National Institute of Standards and Technology (NIST) Standard Reference Materials (SRM) of traceable certificate. The method used is based on Application Bulletin Leco Corporation. The composition used for the experiment varies from 0,0097% to 0,8110% that is 0,0097 ± 0,0014%; 0,0348 ± 0,0013%; 0,1770 ± 0,003% and 0,8110 ± 0,007%. The analysis results for those varied composition are 0,0097 ± 0,000175%; 0,03474 ± 0,000152%; 0,1762 ± 0,00228% and 0,80982 ± 0,000958% for their mean value and standard deviation respectively. In the standard analysis, the results are close to the true value is the measurement of a standard sample with a content of 0.811% with a correction factor of 1.0015. The smallest standard deviation in measurements of 0,0348% sample gives the lowest standard deviation, i.e. 0,000152. The analysis results are considered sufficiently stable with linear calibration curve of y = 0.9984 x with correlation coefficient R 2 = 1. (author)

Decree 212/80 11/4/80 Nuclear politic of the Uruguay eastern Republic; Decreto 212/80 11/4/80 Politica Nuclear de la Republica Oriental del Uruguay

Energy Technology Data Exchange (ETDEWEB)

NONE

1980-07-01

The ordinance 212 of the year 1980 incorporate the science and nuclear technology to the activities techniques scientific and industrials to contribute by means of their application to reach the objectives of economic and social development . It is also included general objective of the political nuclear, lines to reach this objective.

Anti-pancreatic cancer activity of ONC212 involves the unfolded protein response (UPR) and is reduced by IGF1-R and GRP78/BIP.

Science.gov (United States)

Lev, Avital; Lulla, Amriti R; Wagner, Jessica; Ralff, Marie D; Kiehl, Joshua B; Zhou, Yan; Benes, Cyril H; Prabhu, Varun V; Oster, Wolfgang; Astsaturov, Igor; Dicker, David T; El-Deiry, Wafik S

2017-10-10

Pancreatic cancer is chemo-resistant and metastasizes early with an overall five-year survival of ∼8.2%. First-in-class imipridone ONC201 is a small molecule in clinical trials with anti-cancer activity. ONC212, a fluorinated-ONC201 analogue, shows preclinical efficacy in melanoma and hepatocellular-cancer models. We investigated efficacy of ONC201 and ONC212 against pancreatic cancer cell lines ( N =16 including 9 PDX-cell lines). We demonstrate ONC212 efficacy in 4 in-vivo models including ONC201-resistant tumors. ONC212 is active in pancreatic cancer as single agent or in combination with 5-fluorouracil, irinotecan, oxaliplatin or RTK inhibitor crizotinib. Based on upregulation of pro-survival IGF1-R in some tumors, we found an active combination of ONC212 with inhibitor AG1024, including in vivo . We show a rationale for targeting pancreatic cancer using ONC212 combined with targeting the unfolded-protein response and ER chaperones such as GRP78/BIP. Our results lay the foundation to test imipridones, anti-cancer agents, in pancreatic cancer, that is refractory to most drugs.

Scientific Opinion on Flavouring Group Evaluation 212 Revision 3 (FGE.212Rev3): α,β-unsaturated alicyclic ketones and precursors from chemical subgroup 2.6 of FGE.19

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Nørby, Karin Kristiane

The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids of the European Food Safety Authority was requested to evaluate the genotoxic potential of 22 flavouring substances from subgroup 2.6 of FGE.19 in the Flavouring Group Evaluation 212. Based on available genotoxicity dat...

77 FR 67557 - Special Conditions: ATR-GIE Avions de Transport Regional, Models ATR42-500 and ATR72-212A...

Science.gov (United States)

2012-11-13

..., Models ATR42-500 and ATR72-212A Airplanes; Aircraft Electronic System Security Protection From... exploited by unauthorized access to airplane systems, data buses, and servers. Therefore, these special... ATR42-500 and ATR72-212A airplanes. 1. Airplane Electronic System Security Protection from Unauthorized...

Radiobiological Effects of Alpha-Particles from Astatine-211: From DNA Damage to Cell Death

Energy Technology Data Exchange (ETDEWEB)

Claesson, Kristina

2011-05-15

In recent years, the use of high linear energy transfer (LET) radiation for radiotherapeutic applications has gained increased interest. Astatine-211 (211At) is an alpha-particle emitting radionuclide, promising for targeted radioimmunotherapy of isolated tumor cells and microscopic clusters. To improve development of safe radiotherapy using 211At it is important to increase our knowledge of the radiobiological effects in cells. During radiotherapy, both tumors and adjacent normal tissue will be irradiated and therefore, it is of importance to understand differences in the radio response between proliferating and resting cells. The aim of this thesis was to investigate effects in fibroblasts with different proliferation status after irradiation with alpha-particles from 211At or X-rays, from inflicted DNA damage, to cellular responses and biological consequences. Throughout this work, irradiation was performed with alpha-particles from 211A or X-rays. The induction and repair of double-strand breaks (DSBs) in human normal fibroblasts were investigated using pulsed-field gel electrophoresis and fragment analysis. The relative biological effectiveness (RBE) of 211At for DSB induction varied between 1.4 and 3.1. A small increase of DSBs was observed in cycling cells compared to stationary cells. The repair kinetics was slower after 211At and more residual damage was found after 24 h. Comparison between cells with different proliferation status showed that the repair was inefficient in cycling cells with more residual damage, regardless of radiation quality. Activation of cell cycle arrests was investigated using immunofluorescent labeling of the checkpoint kinase Chk2 and by measuring cell cycle distributions with flow cytometry analysis. After alpha-particle irradiation, the average number of Chk2-foci was larger and the cells had a more affected cell cycle progression for several weeks compared with X-irradiated cells, indicating a more powerful arrest after 211At

Relative activities of 212Bi (thorium-C) and 228Ra (mesothorium-1) in former radium dial workers

International Nuclear Information System (INIS)

Keane, A.T.; Essling, M.A.; Lucas, H.F.

1984-01-01

From analyses of bones, estimates were made of the relative intakes of 228 Ra and 226 Ra by the employees of a dial-painting studio that used luminous compounds activated with technical mesothorium. These data, in turn, were used to derive estimates of 228 Ra activity from activities of 226 Ra in living colleagues whose body contents of 226 Ra and 212 Bi had been measured. The indirectly determined 212 Bi to 228 Ra activity ratios in the living workers averaged 1.56 +- 0.10 (S.E.) at a median of 42 yr post intake. If no 228 Ra decay products are excreted, the expected 212 Bi to 228 Ra activity ratio at 42 yr after intake is 1.59 according to the simple power function of radium retention of Norris et al., and 1.72 according to the alkaline earth model of ICRP No. 20. 15 references, 2 tables

36 CFR 212.8 - Permission to cross lands and easements owned by the United States and administered by the Forest...

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Permission to cross lands and easements owned by the United States and administered by the Forest Service. 212.8 Section 212.8 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TRAVEL MANAGEMENT Administration of...

Preparation of radionuclide {sup 212}Pb by emanation method and its subsequent application in radioindicator study of adsorption processes on the bentonite rocks; Priprava radionuklidu {sup 212}Pb emanacnou metodou a jeho nasledna aplikacia pri radioindikatorovom studiu adsorpcnych procesov na bentonitovych horninach

Energy Technology Data Exchange (ETDEWEB)

Viglasova, E; Rosskopfova, O; Galambos, M [Univerzita Komenskeho v Bratislave, Prirodovedecka fakulta, Katedra jadrovej chemie, 84215 Bratislava (Slovakia)

2012-04-25

The paper deals with the preparation of the radionuclide {sup 212}Pb by emanation and its further application in the study of adsorption processes in bentonite rocks. Chronic toxicity of Pb, as of one of the oldest contaminants, causes disturbances in reproductive behaviour, brain damage, neurological disorders, kidney activity damage and many others. {sup 212}Pb has radiogenic origin, it belongs to a series of {sup 232}Th and considering its short half-time time its occurrence is linked primarily to Th. Emanation is a suitable method for preparation of {sup 212}Pb, as a subsidiary radionuclide of a decay series of {sup 232}Th. Due to the electric field at a voltage of 310 V the {sup 212}Pb was captured from Th-source on niobium emanator sheets. Then it leached from there into HNO{sub 3} solution. After adjusting the pH solution and sample preparation there took place a radioindicator study of the effect of mixing time of solid and liquid phase on adsorption percentage of Pb. Adsorption of Pb was studied on bentonite from Slovakian deposits Lieskovec in relation to their use as a tightening barrier of different landfill sites. (authors)

5 CFR 575.212 - Internal monitoring requirements and revocation or suspension of authority.

Science.gov (United States)

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Internal monitoring requirements and... MANAGEMENT CIVIL SERVICE REGULATIONS RECRUITMENT, RELOCATION, AND RETENTION INCENTIVES; SUPERVISORY DIFFERENTIALS; AND EXTENDED ASSIGNMENT INCENTIVES Relocation Incentives § 575.212 Internal monitoring...

27 CFR 25.212 - Beer returned to brewery from which removed.

Science.gov (United States)

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Beer returned to brewery... TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Beer Returned to Brewery § 25.212 Beer returned to brewery from which removed. If beer on which the tax has been determined or paid is returned to...

5 CFR 531.212 - Superior qualifications and special needs pay-setting authority.

Science.gov (United States)

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Superior qualifications and special needs... Appointment Or Position Changes § 531.212 Superior qualifications and special needs pay-setting authority. (a) Agency authority. (1) An agency may use the superior qualifications and special needs pay-setting...

Analysis of survival of C-18 cells after irradiation in suspension with chelated and ionic bismuth-212 using microdosimetry

International Nuclear Information System (INIS)

Stinchcomb, T.G.; Roeske, J.C.

1994-01-01

A previous analysis of non-stochastic dose based on data obtained during irradiations of C-18 cells in suspension by α particles emitted from two forms (chelated and ionic) of 212 Bi was made using survival curves. No appreciable difference in slope (1/D o ) was found between the two forms. Such non-stochastic analyses do not account for the large differences in specific energies deposited in the individual cell nuclei. This microdosimetric (1/z o ) of the individual C-18 cells using the distribution of specific energies deposited in the individual cell nuclei. The resulting sensitivity is greater for the α particles emitted from the chelated 212 Bi than from the ionic 212 Bi. An attempt to account for this greater sensitivity in terms of greater LET of α particles passing through the cell nuclei from the chelated 212 Bi is unsuccessful. Instead the greater sensitivity disappears if the microdosimetric analysis uses average values for the radii of the cell and of its nucleus rather than the values (from the peak in the cell size distribution) used by the non-stochastic dose analysis. 13 refs., 7 figs

WIN 55,212-2, agonist of cannabinoid receptors, prevents amyloid β1-42 effects on astrocytes in primary culture.

Directory of Open Access Journals (Sweden)

Diana Aguirre-Rueda

Full Text Available Alzheimer's disease (AD, a neurodegenerative illness involving synaptic dysfunction with extracellular accumulation of Aβ1-42 toxic peptide, glial activation, inflammatory response and oxidative stress, can lead to neuronal death. Endogenous cannabinoid system is implicated in physiological and physiopathological events in central nervous system (CNS, and changes in this system are related to many human diseases, including AD. However, studies on the effects of cannabinoids on astrocytes functions are scarce. In primary cultured astrocytes we studied cellular viability using MTT assay. Inflammatory and oxidative stress mediators were determined by ELISA and Western-blot techniques both in the presence and absence of Aβ1-42 peptide. Effects of WIN 55,212-2 (a synthetic cannabinoid on cell viability, inflammatory mediators and oxidative stress were also determined. Aβ1-42 diminished astrocytes viability, increased TNF-α and IL-1β levels and p-65, COX-2 and iNOS protein expression while decreased PPAR-γ and antioxidant enzyme Cu/Zn SOD. WIN 55,212-2 pretreatment prevents all effects elicited by Aβ1-42. Furthermore, cannabinoid WIN 55,212-2 also increased cell viability and PPAR-γ expression in control astrocytes. In conclusion cannabinoid WIN 55,212-2 increases cell viability and anti-inflammatory response in cultured astrocytes. Moreover, WIN 55,212-2 increases expression of anti-oxidant Cu/Zn SOD and is able to prevent inflammation induced by Aβ1-42 in cultured astrocytes. Further studies would be needed to assess the possible beneficial effects of cannabinoids in Alzheimer's disease patients.

29 CFR 825.212 - Employee failure to pay health plan premium payments.

Science.gov (United States)

2010-07-01

... Family and Medical Leave Act § 825.212 Employee failure to pay health plan premium payments. (a)(1) In... obligations to maintain health insurance coverage cease under FMLA if an employee's premium payment is more... an employee's insurance in accordance with this section and fails to restore the employee's health...

Radiohalogenation of biomolecules. An experimental study on radiohalogen preparation, precursor synthesis, radiolabeling and biodistribution

International Nuclear Information System (INIS)

Koziorowski, J.

1998-01-01

Radiohalogens are widely used in nuclear medicine, both as tool for diagnostic in vivo imaging, and in radionuclide therapy. This study deals with the use of radiohalogens; separation, precursor synthesis, labeling and biological behavior. The focus is on 211 At and 124 I, the former being a candidate for nuclide therapy and the latter potentially useful for diagnostic imaging and Auger-electron based radiotherapy. For astatine the separation, labeling and some biological behavior is described, and for iodine the latter two. Astatine was separated from an irradiated bismuth target by dry distillation. A novel cryotrap was developed for the isolation of astatine and subsequent synthesis of radiolabeled compounds. 5-[ 211 At]astato-2'-deoxyuridine (AUdR) and N-succinimidyl-4-[ 211 At]astatobenzoate (SAB) were synthesized in 95% respectively 90% radiochemical yields. The former is incorporated into DNA of proliferating cells and can therefore be used as an endoradiotherapeutic agent. The latter is a conjugate for the astatination of proteins. Human epidermal growth factor (hEGF) was tagged with astatine using three approaches: a) direct labeling of native hEGF, b) conjugation with SAB, and c) direct labeling of an hEGF - 7-(3-aminopropyl)-7,8-dicarba-nido-undecaborate(1-) conjugate. The overall labeling yields were 3.5% for direct labeling, 44% for SAB and 70% for the hEGF-nido-carborane conjugate. A new route to N-succinimidyl 3- and 4- [ 124 I]iodobenzoate, two reagents for radioiodination of proteins is described affording 90% radiochemical yield. Three radioiodinated analogs of PK11195, 1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)isoquinoline-3-carboxyam ide, a peripheral-type benzodiazepine receptor antagonist, were synthesized. All three analogs were obtained in >90% radiochemical yield. Synthesis and application of 5-[ 124 I]iodo-2'-deoxyuridine (IUdR) is presented. The closo-dodecaborate anion was evaluated as prosthetic group for radioiodination of

High-spin isomers in 212Rn in the region of triple neutron core-excitations

Science.gov (United States)

Dracoulis, G. D.; Lane, G. J.; Byrne, A. P.; Davidson, P. M.; Kibédi, T.; Nieminen, P.; Watanabe, H.; Wilson, A. N.

2008-04-01

The level scheme of 212Rn has been extended to spins of ∼ 38 ℏ and excitation energies of about 13 MeV using the 204Hg(13C, 5n)212Rn reaction and γ-ray spectroscopy. Time correlated techniques have been used to obtain sensitivity to weak transitions and channel selectivity. The excitation energy of the 22+ core-excited isomer has been established at 6174 keV. Two isomers with τ = 25 (2) ns and τ = 12 (2) ns are identified at 12211 and 12548 keV, respectively. These are the highest-spin nuclear isomers now known, and are attributed to configurations involving triple neutron core-excitations coupled to the aligned valence protons. Semi-empirical shell-model calculations can account for most states observed, but with significant energy discrepancies for some configurations.

High-spin isomers in 212Rn in the region of triple neutron core-excitations

International Nuclear Information System (INIS)

Dracoulis, G.D.; Lane, G.J.; Byrne, A.P.; Davidson, P.M.; Kibedi, T.; Nieminen, P.; Watanabe, H.; Wilson, A.N.

2008-01-01

The level scheme of 212 Rn has been extended to spins of ∼38h and excitation energies of about 13 MeV using the 204 Hg( 13 C, 5n) 212 Rn reaction and γ-ray spectroscopy. Time correlated techniques have been used to obtain sensitivity to weak transitions and channel selectivity. The excitation energy of the 22 + core-excited isomer has been established at 6174 keV. Two isomers with τ=25(2) ns and τ=12(2) ns are identified at 12211 and 12548 keV, respectively. These are the highest-spin nuclear isomers now known, and are attributed to configurations involving triple neutron core-excitations coupled to the aligned valence protons. Semi-empirical shell-model calculations can account for most states observed, but with significant energy discrepancies for some configurations

21 CFR 212.50 - What production and process controls must I have?

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What production and process controls must I have... DRUGS (Eff. 12-12-2011) Production and Process Controls § 212.50 What production and process controls must I have? You must have adequate production and process controls to ensure the consistent production...

Preclinical evaluation of the imipridone family, analogs of clinical stage anti-cancer small molecule ONC201, reveals potent anti-cancer effects of ONC212.

Science.gov (United States)

Wagner, Jessica; Kline, Christina Leah; Ralff, Marie D; Lev, Avital; Lulla, Amriti; Zhou, Lanlan; Olson, Gary L; Nallaganchu, Bhaskara Rao; Benes, Cyril H; Allen, Joshua E; Prabhu, Varun V; Stogniew, Martin; Oster, Wolfgang; El-Deiry, Wafik S

2017-10-02

Anti-cancer small molecule ONC201 upregulates the integrated stress response (ISR) and acts as a dual inactivator of Akt/ERK, leading to TRAIL gene activation. ONC201 is under investigation in multiple clinical trials to treat patients with cancer. Given the unique imipridone core chemical structure of ONC201, we synthesized a series of analogs to identify additional compounds with distinct therapeutic properties. Several imipridones with a broad range of in vitro potencies were identified in an exploration of chemical derivatives. Based on in vitro potency in human cancer cell lines and lack of toxicity to normal human fibroblasts, imipridones ONC206 and ONC212 were prioritized for further study. Both analogs inhibited colony formation, and induced apoptosis and downstream signaling that involves the integrated stress response and Akt/ERK, similar to ONC201. Compared to ONC201, ONC206 demonstrated improved inhibition of cell migration while ONC212 exhibited rapid kinetics of activity. ONC212 was further tested in >1000 human cancer cell lines in vitro and evaluated for safety and anti-tumor efficacy in vivo. ONC212 exhibited broad-spectrum efficacy at nanomolar concentrations across solid tumors and hematological malignancies. Skin cancer emerged as a tumor type with improved efficacy relative to ONC201. Orally administered ONC212 displayed potent anti-tumor effects in vivo, a broad therapeutic window and a favorable PK profile. ONC212 was efficacious in vivo in BRAF V600E melanoma models that are less sensitive to ONC201. Based on these findings, ONC212 warrants further development as a drug candidate. It is clear that therapeutic utility extends beyond ONC201 to include additional imipridones.

Immobilization of Organophosphorus Acid Anhydrolase Mutant Y212F on Silica Nanospheres

Science.gov (United States)

2016-09-01

wild type organophosphorus acid anhydrolase (OPAA) for activity on G-type chemical nerve agents and observed increased activity on the most- toxic ...eventual use in determining particle-binding efficiency and enzymatic activity ..................................................4 5. Fluoride assay...7 TABLES 1. Results of Fluoride Release Assay from DFP Testing of Y212F Enzyme, before and after Conjugation to

Activities of AREVA Med. Extraction and purification of the 212Pb isotope from Thorium for radio-immunotherapy

International Nuclear Information System (INIS)

Miquel, Pierre

2012-01-01

After having recalled the definition of radio-immunotherapy (RIT) and the benefits of alpha RIT for the treatment of some cancers, this document explains the choice of the 212-Pb isotope instead of the 212-Bi isotope (the first one has a longer half-life than the second). The Pb isotope in fact progressively transforms itself into the Bi isotope. The production process is evoked with its important steps. A second part reports the first clinic tests performed in the Alabama Centre for the treatment of different cancer (breast, colon, ovarian, pancreas, stomach). Processes and doses are discussed

20 CFR 1002.212 - How does a person know whether a particular right or benefit is a seniority-based right or benefit?

Science.gov (United States)

2010-04-01

... right or benefit is a seniority-based right or benefit? A seniority-based right or benefit is one that... right or benefit is a seniority-based right or benefit? 1002.212 Section 1002.212 Employees' Benefits... REGULATIONS UNDER THE UNIFORMED SERVICES EMPLOYMENT AND REEMPLOYMENT RIGHTS ACT OF 1994 Reemployment Rights...

Anti-pancreatic cancer activity of ONC212 involves the unfolded protein response (UPR) and is reduced by IGF1-R and GRP78/BIP

OpenAIRE

Lev, Avital; Lulla, Amriti R.; Wagner, Jessica; Ralff, Marie D.; Kiehl, Joshua B.; Zhou, Yan; Benes, Cyril H.; Prabhu, Varun V.; Oster, Wolfgang; Astsaturov, Igor; Dicker, David T.; El-Deiry, Wafik S.

2017-01-01

Pancreatic cancer is chemo-resistant and metastasizes early with an overall five-year survival of ∼8.2%. First-in-class imipridone ONC201 is a small molecule in clinical trials with anti-cancer activity. ONC212, a fluorinated-ONC201 analogue, shows preclinical efficacy in melanoma and hepatocellular-cancer models. We investigated efficacy of ONC201 and ONC212 against pancreatic cancer cell lines (N=16 including 9 PDX-cell lines). We demonstrate ONC212 efficacy in 4 in-vivo models including ON...

Decree 212/80 11/4/80 Nuclear politic of the Uruguay eastern Republic

International Nuclear Information System (INIS)

1980-01-01

The ordinance 212 of the year 1980 incorporate the science and nuclear technology to the activities techniques scientific and industrials to contribute by means of their application to reach the objectives of economic and social development . It is also included general objective of the political nuclear, lines to reach this objective

Radar search and detection with the CASA 212 S43 aircraft

OpenAIRE

Landa Borges, José Manuel

2004-01-01

Approved for public release; distribution in unlimited. This research develops a detection rate model to analyze the effectiveness of the RDR 1500B search radar installed in the CASA 212 S43 aircraft belonging to Venezuelan Naval Aviation. The model is based on a search and detection mission to find a diesel submarine executing an incursion inside the Venezuelan Caribbean Sea area, assumed to be intermittently operating with periscopes or masts exposed above the sea surface. The analysis o...

Activity size distributions of some naturally occurring radionuclides 7Be, 40K and 212Pb in indoor and outdoor environments

International Nuclear Information System (INIS)

Mohamed, A.

2005-01-01

The activity size distributions of natural radionuclides 7 Be and 40 K were measured outdoor in El-Minia city, Egypt by means of gamma spectroscopy. A low-pressure Berner cascade impactor was used as a sampling device. The activity size distribution of both 7 Be and 40 K was described by one log-normal distribution, which was represented by the accumulation mode. The activity median aerodynamic diameter (AMAD) of 7 Be and 40 K was determined to be 530 and 1550nm with a relative geometric standard deviation (δ, which was defined as the dispersion of the peak) of 2.4 and 2, respectively. The same sampling device (Berner impactor) and a screen diffusion battery were used to measure the activity size distribution, activity concentration and unattached fraction (f P ) of 212 Pb in indoor air of El-Minia City, Egypt. The mean activity median aerodynamic diameter (AMAD) of the accumulation mode for attached 212 Pb was determined to be 250nm with a mean geometric standard deviation (δ) of 2.6. The mean value of the specific concentration of 212 Pb associated with that mode was determined to be 460+/-20mBqm -3 . The activity median thermodynamic diameter (AMTD) of unattached 212 Pb was determined to be 1.25nm with δ of 1.4. A mean unattached fraction (f p ) of 0.13+/-0.02 was obtained at a mean aerosol particle concentration of 1.8x10 3 cm -3 . The mean activity concentration of unattached 212 Pb was found to be 19+/-3mBqm -3 . It was found that the aerosol concentration played an important role in varying the unattached, attached activity concentration and unattached fraction (f P )

Sensitization of Tumor to 212Pb Radioimmunotherapy by Gemcitabine Involves Initial Abrogation of G2 Arrest and Blocked DNA Damage Repair by Interference With Rad51

International Nuclear Information System (INIS)

Yong, Kwon Joong; Milenic, Diane E.; Baidoo, Kwamena E.; Brechbiel, Martin W.

2013-01-01

Purpose: To elucidate the mechanism of the therapeutic efficacy of targeted α-particle radiation therapy using 212 Pb-TCMC-trastuzumab together with gemcitabine for treatment of disseminated peritoneal cancers. Methods and Materials: Mice bearing human colon cancer LS-174T intraperitoneal xenografts were pretreated with gemcitabine, followed by 212 Pb-TCMC-trastuzumab and compared with controls. Results: Treatment with 212 Pb-TCMC-trastuzumab increased the apoptotic rate in the S-phase-arrested tumors induced by gemcitabine at earlier time points (6 to 24 hours). 212 Pb-TCMC-trastuzumab after gemcitabine pretreatment abrogated G2/M arrest at the same time points, which may be associated with the inhibition of Chk1 phosphorylation and, in turn, cell cycle perturbation, resulting in apoptosis. 212 Pb-TCMC-trastuzumab treatment after gemcitabine pretreatment caused depression of DNA synthesis, DNA double-strand breaks, accumulation of unrepaired DNA, and down-regulation of Rad51 protein, indicating that DNA damage repair was blocked. In addition, modification in the chromatin structure of p21 may be associated with transcriptionally repressed chromatin states, indicating that the open structure was delayed at earlier time points. Conclusion: These findings suggest that the cell-killing efficacy of 212 Pb-TCMC-trastuzumab after gemcitabine pretreatment may be associated with abrogation of the G2/M checkpoint, inhibition of DNA damage repair, and chromatin remodeling

Thermische Verletzungen im Kindesalter: Eine retrospektive Kohortenstudie von 212 Fällen

OpenAIRE

Sperling, Patrik Leonhart

2012-01-01

Anhand einer retrospektiven Datenanalyse sollen Verteilungsmuster von Verbrennungen und Verbrühungen bezogen auf Alter und Geschlecht untersucht werden. Erfasst wurden 212 Patienten im Alter von 0 bis 16 Jahren betrachtet, die im Zeitraum vom 01.01.2004 bis zum 31.12.2009 auf Grund einer thermischen Verletzung stationär im Universitätsklinikum Würzburg der Julius-Maximilians-Universität Würzburg behandelt wurden. Den größten Anteil thermischer Verletzungen im Kindesalter stellen Verbrühungen ...

Tyrosine 105 and threonine 212 at outermost substrate binding subsites -6 and +4 control substrate specificity, oligosaccharide cleavage patterns, and multiple binding modes of barley alpha-amylase 1

DEFF Research Database (Denmark)

Bak-Jensen, K.S.; André, G.; Gottschalk, T.E.

2004-01-01

and oligosaccharides, respectively. Bond cleavage analysis of oligosaccharide degradation by wild-type and mutant AMY1 supports that Tyr105 is critical for binding at subsite -6. Substrate binding is improved by T212(Y/W) introduced at subsite +4 and the [Y105A/ T212(Y/W)] AMY1 double mutants synergistically enhanced......The role in activity of outer regions in the substrate binding cleft in alpha-amylases is illustrated by mutational analysis of Tyr(105) and Thr(212) localized at subsites - 6 and +4 ( substrate cleavage occurs between subsites -1 and +1) in barley alpha-amylase 1 (AMY1). Tyr(105) is conserved...... in plant alpha-amylases whereas Thr(212) varies in these and related enzymes. Compared with wild-type AMY1, the subsite -6 mutant Y105A has 140, 15, and 1% activity (k(cat)/K-m) on starch, amylose DP17, and 2-chloro-4-nitrophenyl β-D-maltoheptaoside, whereas T212Y at subsite +4 has 32, 370, and 90...

Modeling an emittance-dominated elliptical sheet beam with a 212-dimensional particle-in-cell code

International Nuclear Information System (INIS)

Carlsten, Bruce E.

2005-01-01

Modeling a 3-dimensional (3-D) elliptical beam with a 212-D particle-in-cell (PIC) code requires a reduction in the beam parameters. The 212-D PIC code can only model the center slice of the sheet beam, but that can still provide useful information about the beam transport and distribution evolution, even if the beam is emittance dominated. The reduction of beam parameters and resulting interpretation of the simulation is straightforward, but not trivial. In this paper, we describe the beam parameter reduction and emittance issues related to the initial beam distribution. As a numerical example, we use the case of a sheet beam designed for use with a planar traveling-wave amplifier for high power generator for RF ranging from 95 to 300GHz [Carlsten et al., IEEE Trans. Plasma Sci. 33 (2005) 85]. These numerical techniques also apply to modeling high-energy elliptical bunches in RF accelerators

Sensitization of Tumor to {sup 212}Pb Radioimmunotherapy by Gemcitabine Involves Initial Abrogation of G2 Arrest and Blocked DNA Damage Repair by Interference With Rad51

Energy Technology Data Exchange (ETDEWEB)

Yong, Kwon Joong; Milenic, Diane E.; Baidoo, Kwamena E. [Radioimmune and Inorganic Chemistry Section, Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland (United States); Brechbiel, Martin W., E-mail: martinwb@mail.nih.gov [Radioimmune and Inorganic Chemistry Section, Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland (United States)

2013-03-15

Purpose: To elucidate the mechanism of the therapeutic efficacy of targeted α-particle radiation therapy using {sup 212}Pb-TCMC-trastuzumab together with gemcitabine for treatment of disseminated peritoneal cancers. Methods and Materials: Mice bearing human colon cancer LS-174T intraperitoneal xenografts were pretreated with gemcitabine, followed by {sup 212}Pb-TCMC-trastuzumab and compared with controls. Results: Treatment with {sup 212}Pb-TCMC-trastuzumab increased the apoptotic rate in the S-phase-arrested tumors induced by gemcitabine at earlier time points (6 to 24 hours). {sup 212}Pb-TCMC-trastuzumab after gemcitabine pretreatment abrogated G2/M arrest at the same time points, which may be associated with the inhibition of Chk1 phosphorylation and, in turn, cell cycle perturbation, resulting in apoptosis. {sup 212}Pb-TCMC-trastuzumab treatment after gemcitabine pretreatment caused depression of DNA synthesis, DNA double-strand breaks, accumulation of unrepaired DNA, and down-regulation of Rad51 protein, indicating that DNA damage repair was blocked. In addition, modification in the chromatin structure of p21 may be associated with transcriptionally repressed chromatin states, indicating that the open structure was delayed at earlier time points. Conclusion: These findings suggest that the cell-killing efficacy of {sup 212}Pb-TCMC-trastuzumab after gemcitabine pretreatment may be associated with abrogation of the G2/M checkpoint, inhibition of DNA damage repair, and chromatin remodeling.

Cancer therapy with alpha-emitters labeled peptides.

Science.gov (United States)

Dadachova, Ekaterina

2010-05-01

Actively targeted alpha-particles offer specific tumor cell killing action with less collateral damage to surrounding normal tissues than beta-emitters. During the last decade, radiolabeled peptides that bind to different receptors on the tumors have been investigated as potential therapeutic agents both in the preclinical and clinical settings. Advantages of radiolabeled peptides over antibodies include relatively straightforward chemical synthesis, versatility, easier radiolabeling, rapid clearance from the circulation, faster penetration and more uniform distribution into tissues, and less immunogenicity. Rapid internalization of the radiolabeled peptides with equally rapid re-expression of the cell surface target is a highly desirable property that enhances the total delivery of these radionuclides into malignant sites. Peptides, such as octreotide, alpha-melanocyte-stimulating hormone analogues, arginine-glycine-aspartic acid-containing peptides, bombesin derivatives, and others may all be feasible for use with alpha-emitters. The on-going preclinical work has primarily concentrated on octreotide and octreotate analogues labeled with Bismuth-213 and Astatine-211. In addition, alpha-melanocyte-stimulating hormone analogue has been labeled with Lead-212/Bismuth-212 in vivo generator and demonstrated the encouraging therapeutic efficacy in treatment of experimental melanoma. Obstacles that continue to obstruct widespread acceptance of alpha-emitter-labeled peptides are primarily the supply of these radionuclides and concerns about potential kidney toxicity. New sources and methods for production of these medically valuable radionuclides and better understanding of mechanisms related to the peptide renal uptake and clearance should speed up the introduction of alpha-emitter-labeled peptides into the clinic. Copyright 2010 Elsevier Inc. All rights reserved.

20 CFR 404.212 - Computing your primary insurance amount from your average indexed monthly earnings.

Science.gov (United States)

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Computing your primary insurance amount from... ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Computing Primary Insurance Amounts Average-Indexed-Monthly-Earnings Method of Computing Primary Insurance Amounts § 404.212 Computing your...

Comparison between anti-CEA and anti-HER2 212Pb-labeled mAbs during α-RIT of small volume peritoneal carcinomatosis - Role of activity distribution on therapeutic efficacy and toxicity?

International Nuclear Information System (INIS)

Elgqvist, J.; Boudousq, V.; Bobyk, L.; Busson, M.; Lozza, C.; Navarro-Teulon, I.; Pouget, J.P.; Maquaire, P.; Torgue, J.

2015-01-01

Full text of publication follows. Objectives: we investigated the role of internalizing/non-internalizing monoclonal antibodies (mAbs) on the final outcome (efficacy/toxicity) of mice treated with alpha radioimmunotherapy (α- RIT) using 212 Pb-labeled mAbs. The relationship between distribution of radioactivity at the tissue level and biological parameters was also assessed. Methods: nude mice bearing 2-3 mm peritoneal nodules obtained by xenograft of A-431 tumor cells, expressing low and high level of HER2 and CEA receptors, respectively, were intraperitoneally (i.p.) injected with increasing activities (370-1480 kBq; 37 MBq/mg) of either 35A7 (non-internalising anti-CEA), Trastuzumab (internalizing anti-HER2) or PX (non-specific) 212 Pb-labeled mAbs. Control groups were injected with corresponding amount of unlabeled mAbs or with NaCl. Tumor growth was followed by bioluminescence and median survival (MS) of control and treated mice was determined. 212 Pb-35A7 and 212 Pb-Trastuzumab biodistribution was used to determine the cumulative uptake of radioactivity (UOR) in organs and tumors. Mean absorbed doses were calculated using the MIRD formalism. Haematological, liver and kidney toxicities were also assessed. Distribution of radioactivity at the tissue level was determined by digital micro-autoradiography and the relationship with biological markers of tissue damage was investigated using immunohistochemistry. Results: a mild and transient haematological toxicity in groups treated with the highest amount of activity was observed. MS of the groups treated either with internalizing or non-internalizing 212 Pb-labeled mAbs was significantly improved compared to those treated with non-specific 212 Pb-PX or those only given unlabeled mAbs or just NaCl. MS ranged from 42 days to 94 days using various activity levels of anti-CEA 212 Pb-35A7 while MS was not reached over the follow-up period of 130 days for mice treated with anti-HER2 212 Pb-Trastuzumab. However, UOR and

Angiotensin II Regulates microRNA-132/-212 in Hypertensive Rats and Humans

DEFF Research Database (Denmark)

Eskildsen, Tilde V; Jeppesen, Pia L; Schneider, Mikael

2013-01-01

MicroRNAs (miRNAs), a group of small non-coding RNAs that fine tune translation of multiple target mRNAs, are emerging as key regulators in cardiovascular development and disease. MiRNAs are involved in cardiac hypertrophy, heart failure and remodeling following cardiac infarction; however, mi...... no effect. Taken together, these data suggest that miR-132 and miR-212 are involved in AngII induced hypertension, providing a new perspective in hypertensive disease mechanisms....

Targeted Deletion of miR-132/-212 Impairs Memory and Alters the Hippocampal Transcriptome

Science.gov (United States)

Hansen, Katelin F.; Sakamoto, Kensuke; Aten, Sydney; Snider, Kaitlin H.; Loeser, Jacob; Hesse, Andrea M.; Page, Chloe E.; Pelz, Carl; Arthur, J. Simon C.; Impey, Soren; Obrietan, Karl

2016-01-01

miR-132 and miR-212 are structurally related microRNAs that have been found to exert powerful modulatory effects within the central nervous system (CNS). Notably, these microRNAs are tandomly processed from the same noncoding transcript, and share a common seed sequence: thus it has been difficult to assess the distinct contribution of each…

Cytochrome P4502D6(193-212): a new immunodominant epitope and target of virus/self cross-reactivity in liver kidney microsomal autoantibody type 1-positive liver disease.

Science.gov (United States)

Kerkar, Nanda; Choudhuri, Kaushik; Ma, Yun; Mahmoud, Ayman; Bogdanos, Dimitrios P; Muratori, Luigi; Bianchi, Francesco; Williams, Roger; Mieli-Vergani, Giorgina; Vergani, Diego

2003-02-01

Cytochrome P4502D6 (CYP2D6), target of liver kidney microsomal autoantibody type 1 (LKM1), characterizes autoimmune hepatitis type 2 (AIH2) but is also found in patients with chronic hepatitis C virus (HCV) infection. To provide a complete linear epitope B cell map of CYP2D6, we tested peptides spanning the entire sequence of CYP2D6. In addition to confirming previously described antigenic sites, we identified four new epitopes (193-212, 238-257, 268-287, and 478-497). CYP2D6(193-212) is immunodominant and was the target of 12 of 13 (93%) patients with AIH2 and 5 of 10 (50%) HCV/LKM1-positive patients. Because LKM1 is present in both AIH2 and a viral infection, we tested whether Abs to CYP2D6(193-212) arise through cross-reactive immunity between virus and self. We identified a hexameric sequence "RLLDLA" sharing 5 of 6 aa with "RLLDLS" of HCV(2985-2990) and all 6 aa with CMV(130-135). Of 17 CYP2D6(193-212)-reactive sera, 11 (7 AIH and 4 HCV) reacted by ELISA with the HCV homologue, 8 (5 AIH and 3 HCV) with the CMV homologue, and 8 (5 AIH and 3 HCV) showed double reactivity. Autoantibody binding to CYP2D6(193-212) was inhibited by preincubation with HCV(2977-2996) or CMV(121-140). Recombinant HCV-nonstructural protein 5 and CMV-UL98 proteins also inhibited Ab binding to CYP2D6(193-212). Affinity-purified CYP2D6(193-212)-specific Ab inhibited the metabolic activity of CYP2D6. The demonstrated similarity and cross-reactivity between CYP2D6(193-212) and two unrelated viruses suggests that multiple exposure to viruses mimicking self may represent an important pathway to the development of autoimmunity.

Astatine-211 labeling. A study towards automatic production of astatinated antibodies

International Nuclear Information System (INIS)

Emma Aneheim; Per Albertsson; Sture Lindegren; Holger Jensen

2015-01-01

Targeted alpha therapy is especially interesting for therapy of microscopic cancer tumors due to short path length and high linear energy transfer of the alpha particles. One of the most promising nuclides for targeted alpha therapy is 211 At. To facilitate larger clinical studies using 211 At, the current manual synthesis of radiolabeled antibodies would benefit from being transferred into an automated method. In this work, successful modifications of the manual synthesis have been performed in order to adapt it to automation. The automatic synthesis has also been tested using the modified synthesis method. (author)

Effects of URB597 as an inhibitor of fatty acid amide hydrolase on WIN55, 212-2-induced learning and memory deficits in rats.

Science.gov (United States)

Hasanein, Parisa; Teimuri Far, Massoud

2015-04-01

Cannabinoid and endocannabinoid systems have been implicated in several physiological functions including modulation of cognition. In this study we evaluated the effects and interaction between fatty-acid amide hydrolase (FAAH) inhibitor URB597 and CB1 receptor agonist WIN55, 212-2 on memory using object recognition and passive avoidance learning (PAL) tests. Learning and memory impairment was induced by WIN 55, 212-2 administration (1mg/kg, i.p.) 30min before the acquisition trial. URB597 (0.1, 0.3 and 1mg/kg, i.p.) or SR141716A (1mg/kg, i.p.) was injected to rats 10min before WIN 55, 212-2 or URB597 respectively. URB597 (0.3 and 1mg/kg) but not 0.1mg/kg induced higher discrimination index (DI) in object recognition test and enhanced memory acquisition in PAL test. The cognitive enhancing effect of URB597 was blocked by a CB1 receptor antagonist, SR141716A which at this dose alone had no effect on cognition. WIN55, 212-2 caused cognition deficits in both tests. URB597 (0.3 and 1mg/kg) treatment could alleviate the negative influence of WIN 55, 212-2 on cognition and memory. These results indicate URB597 potential to protect against memory deficits induced by cannabinoid. Therefore, in combination with URB597 beneficial effects, this study suggests that URB597 has recognition and acquisition memory enhancing effects. It may also constitute a novel approach for the treatment of cannabinoid induced memory deficits and lead to a better understanding of the brain mechanisms underlying cognition. Copyright © 2015 Elsevier Inc. All rights reserved.

48 CFR 212.570 - Applicability of certain laws to contracts and subcontracts for the acquisition of commercially...

Science.gov (United States)

2010-10-01

..., DEPARTMENT OF DEFENSE ACQUISITION PLANNING ACQUISITION OF COMMERCIAL ITEMS Applicability of Certain Laws to the Acquisition of Commercial Items 212.570 Applicability of certain laws to contracts and... laws to contracts and subcontracts for the acquisition of commercially available off-the-shelf items...

$\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with radioactive At beams

CERN Multimedia

We propose to study the $\\beta$-delayed fission, laser spectroscopy and radioactive decay of the newly available pure beams of neutron-deficient and neutron-rich astatine (Z=85) isotopes. The fission probability and the fission fragment distribution of the even-even isotopes $^{194,196}$Po following the $\\beta$-decay of the isotopes $^{194,196}$At will be studied with the Windmill setup. In-source laser spectroscopy will be performed on the entire astatine isotopic chain, using a combination of the Windmill setup, ISOLTRAP MR-ToF and ISOLDE Faraday. Radioactive decay data will be acquired at the Windmill setup throughout those studies and contribute to the global understanding of the phenomenon of shape coexistence in the neutron-deficient lead region.

MicroRNA-212 post-transcriptionally regulates oocyte-specific basic-helix-loop-helix transcription factor, factor in the germline alpha (FIGLA, during bovine early embryogenesis.

Directory of Open Access Journals (Sweden)

Swamy K Tripurani

Full Text Available Factor in the germline alpha (FIGLA is an oocyte-specific basic helix-loop-helix transcription factor essential for primordial follicle formation and expression of many genes required for folliculogenesis, fertilization and early embryonic survival. Here we report the characterization of bovine FIGLA gene and its regulation during early embryogenesis. Bovine FIGLA mRNA expression is restricted to gonads and is detected in fetal ovaries harvested as early as 90 days of gestation. FIGLA mRNA and protein are abundant in germinal vesicle and metaphase II stage oocytes, as well as in embryos from pronuclear to eight-cell stage but barely detectable at morula and blastocyst stages, suggesting that FIGLA might be a maternal effect gene. Recent studies in zebrafish and mice have highlighted the importance of non-coding small RNAs (microRNAs as key regulatory molecules targeting maternal mRNAs for degradation during embryonic development. We hypothesized that FIGLA, as a maternal transcript, is regulated by microRNAs during early embryogenesis. Computational predictions identified a potential microRNA recognition element (MRE for miR-212 in the 3' UTR of the bovine FIGLA mRNA. Bovine miR-212 is expressed in oocytes and tends to increase in four-cell and eight-cell stage embryos followed by a decline at morula and blastocyst stages. Transient transfection and reporter assays revealed that miR-212 represses the expression of FIGLA in a MRE dependent manner. In addition, ectopic expression of miR-212 mimic in bovine early embryos dramatically reduced the expression of FIGLA protein. Collectively, our results demonstrate that FIGLA is temporally regulated during bovine early embryogenesis and miR-212 is an important negative regulator of FIGLA during the maternal to zygotic transition in bovine embryos.

Fourier transform infrared spectroscopy of D212CO in the 2500-4500 cm-1 region and the first rovibrational analysis of its v2 = 2 state

Science.gov (United States)

A'dawiah, Rabia'tul; Tan, T. L.; Ng, L. L.

2018-03-01

A low-resolution (0.5 cm-1) Fourier transform infrared (FTIR) spectrum of formaldehyde-d2 (D212CO) in the 2500-4500 cm-1 region was recorded to study the combination bands in this region. The bands ν2 +ν4,ν2 +ν6 , ν2 +ν3 , ν1 +ν2 , ν2 +ν5 , 3ν3 , 2ν2 and 2ν5 were identified and their band centers (with an uncertainty of ± 0.1 cm-1) and band types were determined. Furthermore, the high-resolution FTIR spectrum of the 2ν2 overtone band (3315-3440 cm-1) of D212CO was recorded at an unapodized resolution of 0.0063 cm-1 and its infrared lines were analyzed. A total of 970 rovibrational transitions have been assigned and fitted up to J‧ = 35 and Ka‧ = 14 using the Watson's A-reduced Hamiltonian in the Ir representation. Upper state (v2 = 2) rovibrational constants inclusive of three rotational and five quartic centrifugal distortion constants were accurately determined for the first time. The band center of the 2ν2 band was determined as 3385.200666 ± 0.000035 cm-1. The rms deviation of the rovibrational fit was 0.00093 cm-1. From the fitting of 451 ground state combination differences (GSCDs) of D212CO which were derived from the infrared transitions of the 2ν2 band of this work, together with 360 microwave frequencies from a previous study, new and accurate ground state constants of D212CO up to three octic terms were obtained. The combination and overtone bands and the newly assigned high-resolution infrared lines of the 2ν2 band in the 2500-4500 cm-1 region can be used to detect D212CO in this infrared region. In addition, the results derived from this study give information on the rovibrational molecular structure of D212CO.

21 CFR 212.90 - What actions must I take to control the distribution of PET drug products?

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What actions must I take to control the... POSITRON EMISSION TOMOGRAPHY DRUGS (Eff. 12-12-2011) Distribution § 212.90 What actions must I take to control the distribution of PET drug products? (a) Written distribution procedures. You must establish...

Chromosome aberrations in the peripheral lymphocytes of thorium workers with low body burdens of 212Bi

International Nuclear Information System (INIS)

Hoegerman, S.F.

1976-01-01

Cytogenetic analysis of 8 thorium workers and 3 controls has not shown a significant elevation in the level of chromosome breakage in the workers' peripheral lymphocytes. This finding is consistent with an estimate of the amount of damage to be expected in these cases, based on the level of chromosome breakage observed in Thorotrast cases with measured 212 Bi burdens

Radioimmunoscintigraphy of colorectal carcinoma using technetium-99m-labeled, totally human monoclonal antibody 88BV59H21-2.

Science.gov (United States)

Gulec, S A; Serafini, A N; Moffat, F L; Vargas-Cuba, R D; Sfakianakis, G N; Franceschi, D; Crichton, V Z; Subramanian, R; Klein, J L; De Jager, R L

1995-12-01

Radioimmunoscintigraphy (RIS) using human monoclonal antibodies offers the important clinical advantage of repeated imaging over murine monoclonal antibodies by eliminating the cross-species antibody response. This article reports a Phase I-II clinical trial with Tc-99m-labeled, totally human monoclonal antibody 88BV59H21-2 in patients with colorectal carcinoma. The study population consisted of 34 patients with colorectal cancer (20 men and 14 women; age range, 44-81 years). Patients were administered 5-10 mg antibody labeled with 21-41 mCi Tc-99m by the i.v. route and imaged at 3-10 and 16-24 h after infusion using planar and single-photon emission computed tomographic (CT) techniques. Pathological confirmation was obtained in 25 patients who underwent surgery. Human antihuman antibody (HAHA) titers were checked prior to and 1 and 3 months after the infusion. RIS with Tc-99m-labeled 88BV59H21-2 revealed a better detection rate in the abdomen-pelvis region compared with axial CT. The combined use of both modalities increased the sensitivity in both the liver and abdomen-pelvis regions. Ten patients developed mild adverse reactions (chills and fever). No HAHA response was detected in this series. Tc-99m-labeled human monoclonal antibody 88BV59H21-2 RIS shows promise as a useful diagnostic modality in patients with colorectal cancer. RIS alone or in combination with CT is more sensitive than CT in detecting tumor within the abdomen and pelvis. Repeated RIS studies may be possible, due to the lack of a HAHA response.

21 CFR 212.60 - What requirements apply to the laboratories where I test components, in-process materials, and...

Science.gov (United States)

2010-04-01

... maintenance. Each laboratory must have and follow written procedures to ensure that equipment is routinely... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What requirements apply to the laboratories where...) Laboratory Controls § 212.60 What requirements apply to the laboratories where I test components, in-process...

EFSA CEF Penal (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2014. Scientific Opinion on Flavouring Group Evaluation 212, Revision 2 (FGE.212Rev2): α,β-Unsaturated alicyclic ketones and precursors from chemical subgroup 2.6 of FGE.19

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Binderup, Mona-Lise; Lund, Pia

The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids of the European Food Safety Authority was requested to evaluate the genotoxic potential of 24 flavouring substances from subgroup 2.6 of FGE.19 in the Flavouring Group Evaluation 212, Revision 2. The Panel concluded in ...

21 CFR 212.70 - What controls and acceptance criteria must I have for my finished PET drug products?

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What controls and acceptance criteria must I have... POSITRON EMISSION TOMOGRAPHY DRUGS (Eff. 12-12-2011) Finished Drug Product Controls and Acceptance § 212.70 What controls and acceptance criteria must I have for my finished PET drug products? (a) Specifications...

Immuno-vectorization of radioelements emitters of alpha particles: a new therapy in cancerology

International Nuclear Information System (INIS)

Bourgeois, M.

2007-05-01

The radio-immunotherapy is an anti cancerous therapy which consists in vectorising with immuno-specific agents very radio toxic radioelements on tumors or in their environment to destroy them. The first part of this report presents the different characteristics of antibodies as well as their means of production under monoclonal shapes specifically steered against a tumoral antigen of interest. The second part of this report replaces the importance of the immunological vectors in the context of the nuclear medicine. It is notably described that the different methods which allow to radio-label the vector, as well as the different ways of optimization which were envisaged to improve the targeting of radioelements on a tumor. These different developments allow to define the potential place of the alpha radio-immunotherapy in treatments and so re-place the interest of the experimental part. If the radio-immunotherapy, using beta emitters isotopes as the 131 iodine or the 90 yttrium, is today current in anti cancerous therapy, it finds limits because of the disintegration characteristics of the isotopes it uses. Indeed, compared with alpha particles, the beta particles deposit less energy by unit of length in the crossed material.The experimental part of this report aims at studying the feasibility of the coupling between an immunological vector and an alpha emitter isotope.The different tests led on the bismuth 213, the bismuth 212, the lead 212 and the astatine 211 demonstrated that the fixation of these radionuclides was possible. This research theme is strengthened by the construction in Nantes of a cyclotron with high energy ( A.R.R.O.N.A.X.) and the optimization of the obtained promising results should allow a therapeutic use in oncology of the alpha radio-immunotherapy. (N.C.)

Observation of atmospheric 210Pb and 212Pb originating from the 2004 eruptive activity of Asama volcano, Japan, and relevant 222Rn releasing from the erupting magma

International Nuclear Information System (INIS)

Kukita, Kazuhiko; Koike, Yuya; Nakamura, Toshihiro; Sato, Jun; Saito, Takashi

2005-01-01

This paper describes a study of observation of atmospheric 210Pb and 212Pb possibly from the volcano (36 deg N, 138 deg E) activity in the title and of measurement of 222Rn releasing efficiency with the ash-fall deposit collected around the period. The aerosol sample was collected from Sep. 1, an eruption day, on a building terrace (10 m high) of Meiji University at Kawasaki, located at 140 km SE of the volcano, every 24 hr on the glass fiber filter using a high volume air sampler. The filter was cut out to 4 disks, which were packed into acrylic canisters with a window of a thin Mylar film for non-destructive γ-ray measurement. 210Pb and 212Pb radioactivities were determined by the 46.5- and 238.6-keV γ-rays with an LEPS (low energy photon spectrometer) and an HPGe spectrometer, respectively. The ash-fall sample from the eruption Sep. 14, was collected at Kanrakumachi, Gunma Pref., 40 km SE of the volcano, and measurement for the growth curve of 222Rn from the fall started 1 week after the eruption. A well-type HPGe spectrometer was used for determination of the 351.9-keV γ-ray of 222Rn from 214Pb in equilibrium, which was normalized by the 911.1-keV 228Ac γ-ray. 210Pb and 212Pb emitted into the atmosphere were suggested to have been transported 140 km within the time of a few times of the 212Pb half life (10.6 hr) on the northerly wind. 210Pb and 212Pb, and 222Rn were suggested to be a possibly useful tool of monitoring magmatic activities. (S.I.)

Patio 2.12: Vivienda prefabricada, sostenible, autosuficiente y energéticamente eficiente. Participación en la competición Solar Decathlon Europe 2012

Directory of Open Access Journals (Sweden)

Terrados-Cepeda, F. J.

2015-06-01

Full Text Available The prototype “Patio 2.12” was Andalucía Team’s proposal for the 2012 Solar Decathlon Competition (SDE 2012, that was held in Madrid during September 2012, where it was awarded several prizes like the first one in Energy Efficiency. The team comprised a group of teachers and students from four Andalusian Universities. It worked for two years in the design and construction of a housing prototype that could be an example of several concepts at the same time: energy efficiency, architectural integration of solar systems, intermediate scale of prefabrication and a today´s interpretation of traditional Mediterranean way of living and building. Patio 2.12 was intended to be a compendium of energy, sustainability and passive conditioning devices.El prototipo Patio 2.12 fue la propuesta del equipo Andalucía Team para la competición Solar Decathlon Europe 2012 (SDE 2012, celebrada en Madrid en septiembre de 2012, en la que recibió hasta 9 premios, entre los que figura el de Eficiencia Energética. El equipo, integrado por profesores y alumnos de cuatro universidades andaluzas, trabajó durante dos años en el diseño y construcción de un prototipo de vivienda basada en la eficiencia energética, en la integración arquitectónica de los sistemas solares, en la prefabricación de escala intermedia y en una reinterpretación de la forma de vivir y construir de la tradición mediterránea. Patio 2.12 pretende ser un compendio de energía, sostenibilidad y mecanismos de acondicionamiento pasivo.

An Expert System for Managing Storage Space Constraints Aboard United States Naval Vessels

Science.gov (United States)

1991-12-01

d. solvents, thinners, primers, cmpounds, varnishes , and lacquers i e. alcohol, acetone, ether, and naphtha; f. greases * nd pastes Except for...suffocation. Malocarbon liquids are compounds of carbon containing any of the halogen elements ( fluorine , chlorine, bromine, iodine, or astatine. (Examples are

Primary non-refluxive megaureter in children: single-center experience and follow-up of 212 patients.

Science.gov (United States)

Rubenwolf, P; Herrmann-Nuber, J; Schreckenberger, M; Stein, R; Beetz, R

2016-11-01

Primary non-refluxing megaureter (pMU) is a multifaceted and challenging congenital pathology of the urinary tract. We report our 23-year experience with this anomaly in terms of presentation, diagnostic work-up and management. We retrospectively reviewed the medical records of 212 children diagnosed with pMU between 1986 and 2009 at our institution. Mean follow-up was 45.17 (0-192) months. Of the total, 168 (79 %) children presented with upper urinary tract dilation on perinatal ultrasound screening. In 44 (21 %) patients, the diagnosis was made following diagnostic work-up of a urinary tract infection (UTI, 18 %) or flank pain (3 %). In total, 203 of 254 pMUs (79.9 %) were successfully treated conservatively during the 23-year observation period. Forty-eight children (23 %) underwent ureteric reimplantation. UTIs occurred in 91 of 212 children (43 %). Of these, 41 (45 %) occurred despite antibacterial infection prophylaxis. Within the past three decades, there has been a marked shift from surgical toward conservative therapy at our institution. Neonatal renal ultrasound is the method of choice to timely identify children with pMU and, alongside dynamic renography, to monitor the clinical course. Nowadays, only a minor subset of children with asymptomatic course requires surgical correction. Antibacterial prophylaxis has the potential to reduce the risk of febrile UTIs. Prospective randomized studies are warranted to provide evidence of the beneficial effect of antibacterial prophylaxis.

Alpha particle emitters in cancer therapy: establishing the rationale and overcoming the difficulties

International Nuclear Information System (INIS)

Sgouros, G.

1996-01-01

Full text: Once a tumor has metastasized, the possibility of cure is significantly diminished, if not excluded. Since metastatic spread arises due to the release of single tumor cells or tumor cell clusters, treatment regimens following an overt metastasis must include agents that eradicate individual tumor cells and cell clusters or that prevent their dissemination. Alpha particles may be highly effective in eradicating rapidly accessible disease. The effectiveness of alpha particles arises because the amount of energy deposited per unit distance traveled (linear energy transfer or LET) is approximately 400 times greater than that of beta particles (80 keV/μm vs. 0.2 keV/μm). Each traversal of an alpha particle through a cell nucleus results in a very highly ionizing track. Cell survival studies have shown that alpha-particle killing is independent of oxygenation state or cell-cycle during irradiation and that as few as 1 to 6 tracks across the nucleus may result in cell death. Most studies with alpha-particle emitting radionuclides for therapy have examined either bismuth-212 or astatine-211. Both radionuclides are short-lived with 61 minute and 7.2 hour half-lives, respectively, yielding intermediates with 3-minute and 32 year half-lives, respectively. Both emit alpha particles whose range is 40 to 80 μm. Alpha-particle emitting radionuclides have been attached to antibodies against tumor cell associated antigen. Antibodies have been the most widely used vehicle for delivery of alpha particles due to their specificity. Bismuth-212 has demonstrated a significant curative potential with minimal toxicity. In an ascites tumor mouse model, specific targeting and 80% cure following injection of Bi-212-labeled antibody has been observed (Macklis RM et al, Science, 240:1024-1026, 1988). It is important to define the realm of applicability for alpha particle emitting radionuclides. The short half-life of most currently available radionuclides, limits their use to

Calibrated cryo-cell UV-LA-ICPMS elemental concentrations from the NGRIP ice core reveal abrupt, sub-annual variability in dust across the GI-21.2 interstadial period

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D. Della Lunga

2017-05-01

Full Text Available Several abrupt shifts from periods of extreme cold (Greenland stadials, GS to relatively warmer conditions (Greenland interstadials, GI called Dansgaard–Oeschger events are recorded in the Greenland ice cores. Using cryo-cell UV-laser-ablation inductively coupled-plasma mass spectrometry (UV-LA-ICPMS, we analysed a 2.85 m NGRIP ice core section (2691.50–2688.65 m depth, age interval 84.86–85.09 ka b2k, thus covering  ∼  230 years across the transitions of GI-21.2, a short-lived interstadial prior to interstadial GI-21.1. GI-21.2 is a  ∼  100-year long period with δ18O values 3–4 ‰ higher than the following  ∼  200 years of stadial conditions (GS-21.2, which precede the major GI-21.1 warming. We report concentrations of major elements indicative of dust and/or sea salt (Na, Fe, Al, Ca, Mg at a spatial resolution of  ∼  200 µm, while maintaining detection limits in the low-ppb range, thereby achieving sub-annual time resolution even in deep NGRIP ice. We present an improved external calibration and quantification procedure using a set of five ice standards made from aqueous (international standard solutions. Our results show that element concentrations decrease drastically (more than 10-fold at the warming onset of GI-21.2 at the scale of a single year, followed by relatively low concentrations characterizing the interstadial part before gradually reaching again typical stadial values.

Charge radii and electromagnetic moments of At-211195

Science.gov (United States)

Cubiss, J. G.; Barzakh, A. E.; Seliverstov, M. D.; Andreyev, A. N.; Andel, B.; Antalic, S.; Ascher, P.; Atanasov, D.; Beck, D.; Bieroń, J.; Blaum, K.; Borgmann, Ch.; Breitenfeldt, M.; Capponi, L.; Cocolios, T. E.; Day Goodacre, T.; Derkx, X.; De Witte, H.; Elseviers, J.; Fedorov, D. V.; Fedosseev, V. N.; Fritzsche, S.; Gaffney, L. P.; George, S.; Ghys, L.; Heßberger, F. P.; Huyse, M.; Imai, N.; Kalaninová, Z.; Kisler, D.; Köster, U.; Kowalska, M.; Kreim, S.; Lane, J. F. W.; Liberati, V.; Lunney, D.; Lynch, K. M.; Manea, V.; Marsh, B. A.; Mitsuoka, S.; Molkanov, P. L.; Nagame, Y.; Neidherr, D.; Nishio, K.; Ota, S.; Pauwels, D.; Popescu, L.; Radulov, D.; Rapisarda, E.; Revill, J. P.; Rosenbusch, M.; Rossel, R. E.; Rothe, S.; Sandhu, K.; Schweikhard, L.; Sels, S.; Truesdale, V. L.; Van Beveren, C.; Van den Bergh, P.; Wakabayashi, Y.; Van Duppen, P.; Wendt, K. D. A.; Wienholtz, F.; Whitmore, B. W.; Wilson, G. L.; Wolf, R. N.; Zuber, K.

2018-05-01

Hyperfine-structure parameters and isotope shifts of At-211195 have been measured for the first time at CERN-ISOLDE, using the in-source resonance-ionization spectroscopy method. The hyperfine structures of isotopes were recorded using a triad of experimental techniques for monitoring the photo-ion current. The Multi-Reflection Time-of-Flight Mass Spectrometer, in connection with a high-resolution electron multiplier, was used as an ion-counting setup for isotopes that either were affected by strong isobaric contamination or possessed a long half-life; the ISOLDE Faraday cups were used for cases with high-intensity beams; and the Windmill decay station was used for short-lived, predominantly α -decaying nuclei. The electromagnetic moments and changes in the mean-square charge radii of the astatine nuclei have been extracted from the measured hyperfine-structure constants and isotope shifts. This was only made possible by dedicated state-of-the-art large-scale atomic computations of the electronic factors and the specific mass shift of atomic transitions in astatine that are needed for these extractions. By comparison with systematics, it was possible to assess the reliability of the results of these calculations and their ascribed uncertainties. A strong deviation in the ground-state mean-square charge radii of the lightest astatine isotopes, from the trend of the (spherical) lead isotopes, is interpreted as the result of an onset of deformation. This behavior bears a resemblance to the deviation observed in the isotonic polonium isotopes. Cases for shape coexistence have been identified in At,199197, for which a significant difference in the charge radii for ground (9 /2- ) and isomeric (1 /2+ ) states has been observed.

Measurements of dry deposition rates of 212Pb from aerosols on various natural and artificial surfaces

International Nuclear Information System (INIS)

Osaki, S.; Sugihara, S.; Maeda, Y.; Osaki, T.

2007-01-01

The dry deposition rates on various grass fields and two forests have been measured by the use of 212 Pb (T 1/2 = 10.6 hours). The deposition rate on grass fields (average: 7 mm x s -1 ) roughly depends on the logarithms of the heights or densities of the grasses. The dry deposition rates on a broadleaved forest (Lithocarpus edulis) and a coniferous forest (Cryptomeria Japonica) were also measured. The highest (ave. 26 mm x s -1 ) was on the forest of C. Japonica because of the dense and adhesive surfaces of the leaves. (author)

Workshop on selected aspects of radiochemistry

International Nuclear Information System (INIS)

1991-11-01

The aspects chosen for the workshop are: isotope preparation, separation methods; radiochemical methods and analyses; environmental protection and radiochemistry; the chemistry of the fifth halogen, astatine. From the 28 contributions presented at the workshop, 24 are of relevance in the INIS and EDB scope and are separately retrievable from the database. (BBR) [de

13CO2/12CO2 ratio analysis in exhaled air by lead-salt tunable diode lasers for noninvasive diagnostics in gastroenterology

Science.gov (United States)

Stepanov, Eugene V.; Zyrianov, Pavel V.; Miliaev, Valerii A.; Selivanov, Yurii G.; Chizhevskii, Eugene G.; Os'kina, Svetlana; Ivashkin, Vladimir T.; Nikitina, Elena I.

1999-07-01

An analyzer of 13CO2/12CO2 ratio in exhaled air based on lead-salt tunable diode lasers is presented. High accuracy of the carbon isotope ratio detection in exhaled carbon dioxide was achieved with help of very simple optical schematics. It was based on the use of MBE laser diodes operating in pulse mode and on recording the resonance CO2 absorption at 4.2 micrometers . Special fast acquisition electronics and software were applied for spectral data collection and processing. Developed laser system was tested in a clinical train aimed to assessment eradication efficiency in therapy of gastritis associated with Helicobacter pylori infection. Data on the 13C-urea breath test used for P.pylori detection and obtained with tunable diode lasers in the course of the trail was compared with the results of Mass-Spectroscopy analysis and histology observations. The analyzer can be used also for 13CO2/12CO2 ratio detection in exhalation to perform gastroenterology breath test based on using other compounds labeled with stable isotopes.

SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

Energy Technology Data Exchange (ETDEWEB)

Jaszczak, R.J.

1995-12-01

Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons.

SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

International Nuclear Information System (INIS)

Jaszczak, R.J.

1995-12-01

Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons

[A commentary on the Ruling of the Tribuna Constitucional 212/1996 of 19 December 1996 (I)].

Science.gov (United States)

González Morán, L

1998-01-01

This article is a commentary on Spain's Constitutional Court's ruling of 19 December 1996 (STC 212/1996), on the challenge (596/89) on grounds of alleged unconstitutionality made against Law 42/1988, 28 December, which regulates the donation of human embryos and foetuses or the cells, tissues and organs therefrom. The article is structured as follows: it opens with a summary of Law 42/1988, since this is felt necessary to understand the subsequent challenge made on grounds of alleged unconstitutionality. We then provide specific details of the challenge and the resulting ruling, before concluding with some critical remarks on the aforementioned Law and ruling.

High spectral resolution observations of the H2 2.12 micron line in Herbig-Haro objects

International Nuclear Information System (INIS)

Zinnecker, H.; Mundt, R.; Geballe, T.R.; Zealey, W.J.

1989-01-01

High-spectral-resolution Fabry-Perot observations of the H 2 2.12-micron line emissions of several Herbig-Haro (HH) objects are discussed. It is shown that H 2 emission by the shock heating of external molecular gas in the wings of the bow shock associated with the working surface of a high-velocity jet may occur for HH objects associated with the jet's end. The shock heating of external molecular gas entrained in the flow by internal shocks occurring in the jet itself and/or in its boundary layer may be the H 2 emission mechanism for HH objects observed along the flow axis. 59 refs

Étude des dommages induits dans l'ADN par irradiation laser X-UV à 21.2 nm

Science.gov (United States)

Cassou, K.; Ros, D.; Kazamias, S.; Klisnick, A.; Jamelot, G.; Guilbaud, O.; Rus, B.; Kozlová, M.; Polan, J.; Präg, A. R.; Stupka, M.; Eot-Houllier, G.; Sage, E.; Begusová, M.; Stísová, V.; Du Penhoat, M.-A. H.; Touati, A.; Chétioui, A.

2005-06-01

We report the preliminary result of the application of the collisional Ne-like soft x-ray laser as radiation source to induce DNA damage. The goal of this experiment was a test bed study of the damage yields induced by soft x-ray radiation in dried plasmid DNA sample. The saturated Ne-like soft x-ray laser available at the PALS facility, delivering several millijoules in a single 100 ps pulse at 21.2 nm was used to irradiate two different plasmids: pSP189 and pBS. The study is centered on the dose effect leading to single and double strand break in DNA.

Determination of {sup 55}Mn(n,γ){sup 56}Mn reaction cross-section at the neutron energies of 1.12, 2.12, 3.12 and 4.12 MeV

Energy Technology Data Exchange (ETDEWEB)

Vansola, Vibha; Mukherjee, Surjit [M.S. University of Baroda, Vadodara (India). Dept. of Physics; Naik, Haladhara [Bhabha Atomic Research Center, Mumbai (India). Radiochemistry Div.; Suryanarayana, Saraswatula Venkata [Bhabha Atomic Research Center, Mumbai (India). Nuclear Physics Div.; Ghosh, Reetuparna; Badwar, Sylvia; Lawriniang, Bioletty Mary [North Eastern Hill Univ., Meghalaya (India). Dept. of Physics; Sheela, Yerraguntla Santhi [Manipal Univ. (India). Dept. of Statistics

2016-07-01

The {sup 55}Mn(n,γ){sup 56}Mn reaction cross-sections at the neutron energies of 1.12, 2.12, 3.12 and 4.12 MeV were determined by using activation and off-line γ-ray spectrometric technique. The neutron energies of 1.12 and 2.12 MeV were generated from the {sup 7}Li(p,n) reaction by using the proton energies of 3 and 4 MeV from the folded tandem ion beam accelerator (FOTIA) at BARC. For the neutron energies of 3.12 and 4.12 MeV, the proton energies used were 5 and 6 MeV from the Pelletron facility at TIFR, Mumbai. The {sup 115}In(n,γ){sup 116m}In reaction cross-section was used as the neutron flux monitor. The {sup 55}Mn(n,γ){sup 56}Mn reaction cross-section at the neutron energies of 4.12 MeV are reported for the first time, whereas at 1.12, 2.12 and 3.12 MeV, they are in between the literature data. The {sup 55}Mn(n,γ){sup 56}Mn reaction cross-section was also calculated theoretically by using the computer code TALYS 1.6 and EMPIRE 3.2.2. The experimental data of present work are found to be in between the theoretical values of TALYS and EMPIRE.

Measuring Progress on the Control of Porcine Reproductive and Respiratory Syndrome (PRRS at a Regional Level: The Minnesota N212 Regional Control Project (Rcp as a Working Example.

Directory of Open Access Journals (Sweden)

Pablo Valdes-Donoso

Full Text Available Due to the highly transmissible nature of porcine reproductive and respiratory syndrome (PRRS, implementation of regional programs to control the disease may be critical. Because PRRS is not reported in the US, numerous voluntary regional control projects (RCPs have been established. However, the effect of RCPs on PRRS control has not been assessed yet. This study aims to quantify the extent to which RCPs contribute to PRRS control by proposing a methodological framework to evaluate the progress of RCPs. Information collected between July 2012 and June 2015 from the Minnesota Voluntary Regional PRRS Elimination Project (RCP-N212 was used. Demography of premises (e.g. composition of farms with sows = SS and without sows = NSS was assessed by a repeated analysis of variance. By using general linear mixed-effects models, active participation of farms enrolled in the RCP-N212, defined as the decision to share (or not to share PRRS status, was evaluated and used as a predictor, along with other variables, to assess the PRRS trend over time. Additionally, spatial and temporal patterns of farmers' participation and the disease dynamics were investigated. The number of farms enrolled in RCP-N212 and its geographical coverage increased, but the proportion of SS and NSS did not vary significantly over time. A significant increasing (p<0.001 trend in farmers' decision to share PRRS status was observed, but with NSS producers less willing to report and a large variability between counties. The incidence of PRRS significantly (p<0.001 decreased, showing a negative correlation between degree of participation and occurrence of PRRS (p<0.001 and a positive correlation with farm density at the county level (p = 0.02. Despite a noted decrease in PRRS, significant spatio-temporal patterns of incidence of the disease over 3-weeks and 3-kms during the entire study period were identified. This study established a systematic approach to quantify the effect of RCPs on

G2M arrest and apoptosis in murine T lymphoma cells following exposure to 212Bi alpha particle irradiation

International Nuclear Information System (INIS)

Palayoor, S.T.; Humm, J.L.; Macklis, R.M.

1993-01-01

Asynchronous exponentially growing EL4 murine T lymphoma cells were exposed either to high LET α-radiation from 212 Bi-DTPA or to γ-radiation from a 137 Cs source. Radiation-induced cell cycle perturbation was studied by flow cytometry. Alpha irradiation, like γ, transiently arrested cells in the G2M phase in a dose-dependent manner. The maximum percentages of cells accumulated in G2M 18 h after α- and γ-irradiation were comparable, though the dose-response relationships differed. The ''RBE'' value for G2M block for α- versus γ-radiation was approx. 4. (author)

The Installation Restoration Program Toxicology Guide. Volume 2

Science.gov (United States)

1987-05-01

Periodic Table: fluorine , chlorine, bromine, iodine, and astatine. Fluorine is the most active of all chemical elements. 5/87 LIST OF ABBREVIATIONS...fire- retardant varnishes and as an aminizing agent for cotton fabric (901). 37.2 ENVIRONMENTAL FATE A1D EXPOSURE PATHWAYS 37.2.1 Transport in Soil...subject to packaging and labeling regulations. Directive on Paints, Varnishes . Printing Inks. Adhesives and Similar Product; (1334) Pentachlorophenol

Diseño de un modelo para la optimización de los procesos en el mantenimiento programado aplicado al equipo Casa 212 de la Fuerza Aérea Colombiana

Directory of Open Access Journals (Sweden)

José Enrique Sarmiento

2006-01-01

Full Text Available Este proyecto es el resultado de Ia busqueda constante de un mejoramiento continuo de los procesos que garantizan la calidad total en el mantenimiento aeronautico, concebdo y desarrollado para ser aplicado en el mantenimiento basico de 100 horas o 1A del equipo C-212-300 asignado al grupo técnico del Comando Aéreo de Combate No.2. Es así como se hace un recuento acerca de Ia historia y Ia evolución del mantenimiento aeronautico, Ia normatividad y los metodos de trabajo, el estudio de tiempos y movimientos empleados actualmente, para garantizar Ia eficiencia y seguridad en Ia aviación comercial y militar. De ahí, que se visitó la Base Aerea del Ejercito del Aire Español, SATENA y la Base Aerea de Apiay de Ia Fuerza Aerea Colombiana, quienes t ienen dentro de sus flotas el equipo C-212.

Electrostatics of the photosynthetic bacterial reaction center. Protonation of Glu L 212 and Asp L 213 - A new method of calculation.

Science.gov (United States)

Ptushenko, Vasily V; Cherepanov, Dmitry A; Krishtalik, Lev I

2015-12-01

Continuum electrostatic calculation of the transfer energies of anions from water into aprotic solvents gives the figures erroneous by order of magnitude. This is due to the hydrogen bond disruption that suggests the necessity to reconsider the traditional approach of the purely electrostatic calculation of the transfer energy from water into protein. In this paper, the method combining the experimental estimates of the transfer energies from water into aprotic solvent and the electrostatic calculation of the transfer energies from aprotic solvent into protein is proposed. Hydrogen bonds between aprotic solvent and solute are taken into account by introducing an imaginary aprotic medium incapable to form hydrogen bonds with the solute. Besides, a new treatment of the heterogeneous intraprotein dielectric permittivity based on the microscopic protein structure and electrometric measurements is elaborated. The method accounts semi-quantitatively for the electrostatic effect of diverse charged amino acid substitutions in the donor and acceptor parts of the photosynthetic bacterial reaction center from Rhodobacter sphaeroides. Analysis of the volatile secondary acceptor site QB revealed that in the conformation with a minimal distance between quinone QB and Glu L 212 the proton uptake upon the reduction of QB is prompted by Glu L 212 in alkaline and by Asp L 213 in slightly acidic regions. This agrees with the pH dependences of protonation degrees and the proton uptake. The method of pK calculation was applied successfully also for dissociation of Asp 26 in bacterial thioredoxin. Copyright © 2015 Elsevier B.V. All rights reserved.

A simple method for labelling proteins with 211At via diazotized aromatic diamine

International Nuclear Information System (INIS)

Wunderlich, G.; Franke, W.-G.; Fischer, S.; Dreyer, R.

1987-01-01

A simple and rapid method for labelling proteins with 211 At by means of a 1,4-diaminobenzene link is described. This link is transformed into the diazonium salt and subsequently reactions of both 211 At and proteins with the diazonium salt take place simultaneously. For possibly high yields of astatized protein an appropriate temperature of 273 K was found. The results demonstrate the difference between the reaction mechanisms of iodine and astatine with proteins. (author)

Hardware-related infections after deep brain stimulation surgery: review of incidence, severity and management in 212 single-center procedures in the first year after implantation.

Science.gov (United States)

Piacentino, Massimo; Pilleri, Manuela; Bartolomei, Luigi

2011-12-01

Device-related infection is a common occurrence after deep brain stimulation (DBS) surgery, and may result in additional interventions and a loss of efficacy of therapy. This retrospective review aimed to evaluate the incidence, severity and management of device-related infections in 212 DBS procedures performed in our institute. Data on 106 patients, in whom 212 DBS procedures were performed between 2001 and 2011 at our institute by a single neurosurgeon (M.P.), were reviewed to assess the incidence, severity, management and clinical characteristics of infections in the first year after the implantation of a DBS system. Infections occurred in 8.5% of patients and 4.2% of procedures. Of the nine infections, eight involved the neurostimulator and extensions, and one the whole system. The infections occurred 30.7 days after implantation: 7 within 30 days and 2 within 6 months. Infected and uninfected patients were comparable in terms of age, sex, indication for DBS implantation and neurostimulator location. In eight cases, the system components involved were removed and re-implanted after 3 months, while in one case the complete hardware was removed and not re-implanted. The overall incidence of postoperative infections after DBS system implantation was 4.2%; this rate decreased over time. All infections required further surgery. Correct and timely management of partial infections may result in successful salvage of part of the system.

Measurements of dry-deposition rates on various earth surfaces by 212Pb

International Nuclear Information System (INIS)

Osaki, S.; Sugihara, S.; Maeda, Y.

2004-01-01

Dry deposition rates of 212 Pb on a coniferous forest (Japanese cedar) and a broad-leaf forest (Pasania edulis) have been measured. Those on various kinds of grass fields, various states on artificial surface such as water, paper, and standing paper have been also measured. The dry deposition rates depend on the characteristics of depositing particles and the conditions of deposited surfaces. Dry deposition rates on the forest of Japanese cedar are highest because of the complex and adhesive surface of the leaves. Those on various grass fields are roughly depend on the logarithm of the height of their grasses. The total deposition rates of 7 Be do not depend on the densities or heights of the grasses. 7 Be may be not kept on their leaves or surface soil for a long time. The dry deposition rates of on artificial surface, e.g. paper and water surfaces make clear the mechanism on dry deposition, and suggest that more chances of collision and more adhesive of the surface are important for the dry deposition. About 90% of all deposition on the artificial paper grass was attached on the standing paper. On water surface, 60% of the rate of paper grass was attached, but only about 20% were attached on a dry paper plate. The aerosol particles are deposited by collision with the surface, therefore the deposition velocity depends on the chance of collision and the characteristics of the surface. Therefore the dry deposition rates on forests are larger and those of coniferous forest are largest. (author)

Remaining Sites Verification Package for the 100-B-21:2 Subsite (100-B/C Discovery Pipeline DS-100BC-002). Attachment Waste Site Reclassification Form 2008-003

International Nuclear Information System (INIS)

Capron, J.M.

2008-01-01

The 100-B-21:2 waste site consists of the immediate area of the DS-100BC-02 pipeline. In accordance with this evaluation, the confirmatory and verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River

A new nonsyndromic X-linked sensorineural hearing impairment linked to Xp21.2

Energy Technology Data Exchange (ETDEWEB)

Lalwani, A.K.; Brister, J.R.; Fex, J.; Grundfast, K.M.; Pikus, A.T.; Ploplis, B.; San Agustin, T.; Skarka, H.; Wilcox, E.R. [National Institutes of Health, Bethesda, MD (United States)

1994-10-01

X-linked deafness is a rare cause of hereditary hearing impairment. We have identified a family with X-linked dominant sensorineural hearing impairment, characterized by incomplete penetrance and variable expressivity in carrier females, that is linked to the Xp21.2, which contains the Duchenne muscular dystrophy (DMD) locus. The auditory impairment in affected males was congenital, bilateral, profound, sensorineural, affecting all frequencies, and without evidence of radiographic abnormality of the temporal bone. Adult carrier females manifested bilateral, mild-to-moderate high-frequency sensorineural hearing impairment of delayed onset during adulthood. Eighteen commercially available polymorphic markers from the X chromosome, generating a 10-15-cM map, were initially used for identification of a candidate region. DXS997, located within the DMD gene, generated a two-point LOD score of 2.91 at {theta} = 0, with every carrier mother heterozygous at this locus. Recombination events at DXS992 (located within the DMD locus, 3{prime} to exon 50 of the dystrophin gene) and at DXS1068 (5{prime} to the brain promoter of the dystrophin gene) were observed. No recombination events were noted with the following markers within the DMD locus: 5{prime}DYS II, intron 44, DXS997, and intron 50. There was no clinical evidence of Duchenne or Becker muscular dystrophy in any family member. It is likely that this family represents a new locus on the X chromosome, which when mutated results in nonsyndromic sensorineural hearing loss and is distinct from the heterogeneous group of X-linked hearing losses that have been previously described. 57 refs., 6 figs., 1 tab.

Reagents for Astatination of Biomolecules. 5. Evaluation of hydrazone linkers in 211At- and 125I-labeled closo-decaborate(2-) conjugates of Fab′ as a means of decreasing kidney retention

Science.gov (United States)

Wilbur, D. Scott; Chyan, Ming-Kuan; Hamlin, Donald K.; Nguyen, Holly; Vessella, Robert L.

2011-01-01

Evaluation of monoclonal antibody (MAb) fragments (e.g. Fab′, Fab or engineered fragments) as cancer-targeting reagents for therapy with the α-particle emitting radionuclide astatine-211 (211At) has been hampered by low in vivo stability of the label and a propensity of these proteins localize to kidneys. Fortunately, our group has shown that the low stability of the 211At label, generally a meta- or para-[211At]astatobenzoyl conjugate, on MAb Fab′ fragments can be dramatically improved by use of closo-decaborate(2-) conjugates. However, the higher stability of radiolabeled MAb Fab′ conjugates appears to result in retention of the radioactivity in kidneys. This investigation was conducted to evaluate whether the retention of radioactivity in kidney might be decreased by the use of acid-cleavable hydrazone between the Fab′ and the radiolabeled closo-decaborate(2-) moiety. Five conjugation reagents containing sulfhydryl-reactive maleimide groups, a hydrazone functionality and a closo-decaborate(2-) moiety were prepared. In four of the five conjugation reagents, a discrete polyethylene glycol (PEG) linker was used, and one substituent adjacent to the hydrazone was varied (phenyl, benzoate, anisole or methyl) to provide varying acid-sensitivity. In the initial studies, the five maleimido-closo-decaborate(2-) conjugation reagents were radioiodinated (125I or 131I), then conjugated with an anti-PSMA Fab′ (107-1A4 Fab′). Biodistributions of the five radioiodinated Fab′ conjugates were obtained in nude mice at 1, 4 and 24 h post injection (pi). In contrast to closo-decaborate(2-) conjugated to 107-1A4 Fab′ through a non-cleavable linker, two conjugates containing either a benzoate or a methyl substituent on the hydrazone functionality displayed clearance rates from kidney, liver and spleen that were similar to those obtained with directly radioiodinated Fab′ (i.e. no conjugate). The maleimido-closo-decaborate(2-) conjugation reagent containing a benzoate

Decontamination and decommissioning of 61 plutonium gloveboxes in D-Wing, Building 212 Argonne National Laboratory-East: Final project report

International Nuclear Information System (INIS)

Cheever, C.L.; Rose, R.W.

1996-09-01

Argonne National Laboratory-East (ANL-E) is a government-owned, contractor operated, multipurpose research facility located 25 miles southwest of downtown Chicago on 689 hectares (1,700 acres) in DuPage County, Illinois, as shown in Figure 1.1. Building 212 is located in the central area of ANL-E, as shown in Figure 1.2. The purpose of this project was to eliminate the risk of radioactive material release from the contaminated glovebox systems and to make the laboratories available for unrestricted use. The following work objectives were established: (1) Identify and remove radioactive materials for return to ANL-E Special Materials control. (2) Remove and package the radioactively contaminated materials and equipment from the gloveboxes. (3) Decontaminate the gloveboxes to nontransuranic (non-TRU) levels. (4) Size-reduce and package the gloveboxes and support systems. (5) Document and dispose of the radioactive and mixed waste. (6) Decontaminate, survey, and release the nine laboratories and corridor areas for unrestricted use

WIN 55,212-2 Inhibits the Epithelial Mesenchymal Transition of Gastric Cancer Cells via COX-2 Signals

Directory of Open Access Journals (Sweden)

Xiangshu Xian

2016-11-01

Full Text Available Background: Cannabinoids (the active components of Cannabis sativa and their derivatives have received considerable interest due to reports that they can affect the tumor growth, migration, and metastasis. Previous studies showed that the cannabinoid agonist WIN 55,212-2 (WIN was associated with gastric cancer (GC metastasis, but the mechanisms were unknown. Methods: The effects of WIN on GC cell migration and invasion were analyzed by the wound-healing assay and Transwell assay. Quantitative real-time PCR and Western blot were used to evaluate changes in expression of COX-2 and EMT associated markers in SGC7901 and AGS cells. Results: WIN inhibited cell migration, invasion, and epithelial to mesenchymal transition (EMT in GC. WIN treatment resulted in the downregulation of cyclooxygenase-2 (COX-2 expression and decreased the phosphorylation of AKT, and inhibited EMT in SGC7901 cells. Decreased expression of COX-2 and vimentin, and increased expression of E-cadherin, which was induced by WIN, were normalized by overexpression of AKT, suggesting that AKT mediated, at least partially, the WIN suppressed EMT of GC cells. Conclusion: WIN can inhibit the EMT of GC cells through the downregulation of COX-2.

Formation and Atmosphere of Complex Organic Molecules of the HH 212 Protostellar Disk

Energy Technology Data Exchange (ETDEWEB)

Lee, Chin-Fei; Ho, Paul T. P.; Hirano, Naomi; Shang, Hsien [Academia Sinica Institute of Astronomy and Astrophysics, P.O. Box 23-141, Taipei 106, Taiwan (China); Li, Zhi-Yun [Astronomy Department, University of Virginia, Charlottesville, VA 22904 (United States); Zhang, Qizhou, E-mail: cflee@asiaa.sinica.edu.tw [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States)

2017-07-01

HH 212 is a nearby (400 pc) Class 0 protostellar system recently found to host a “hamburger”-shaped dusty disk with a radius of ∼60 au, deeply embedded in an infalling-rotating flattened envelope. We have spatially resolved this envelope-disk system with the Atacama Large Millimeter/submillimeter Array at up to ∼16 au (0.″04) resolution. The envelope is detected in HCO{sup +} J = 4–3 down to the dusty disk. Complex organic molecules (COMs) and doubly deuterated formaldehyde (D{sub 2}CO) are detected above and below the dusty disk within ∼40 au of the central protostar. The COMs are methanol (CH{sub 3}OH), deuterated methanol (CH{sub 2}DOH), methyl mercaptan (CH{sub 3}SH), and formamide (NH{sub 2}CHO, a prebiotic precursor). We have modeled the gas kinematics in HCO{sup +} and COMs and found a centrifugal barrier (CB) at a radius of ∼44 au, within which a Keplerian rotating disk is formed. This indicates that HCO{sup +} traces the infalling-rotating envelope down to the CB and COMs trace the atmosphere of a Keplerian rotating disk within the CB. The COMs are spatially resolved for the first time, both radially and vertically, in the atmosphere of a disk in the earliest, Class 0 phase of star formation. Our spatially resolved observations of COMs favor their formation in the disk rather than a rapidly infalling (warm) inner envelope. The abundances and spatial distributions of the COMs provide strong constraints on models of their formation and transport in low-mass star formation.

Measurement of {sup 197}Au(n,γ){sup 198g}Au reaction cross-section at the neutron energies of 1.12, 2.12, 3.12 and 4.12 MeV

Energy Technology Data Exchange (ETDEWEB)

Vansola, Vibha [M.S. Univ., Baroda (India). Dept. of Physics; Ghosh, Reetuparna; Badwar, Sylvia [North Eastern Hill Univ., Meghalaya (India). Dept. of Physics; and others

2015-07-01

The {sup 197}Au(n,γ){sup 198}Au reaction cross-sections at the neutron energies of 1.12, 2.12, 3.12 and 4.12 MeV were determined by using activation and off-line γ-ray spectrometric technique. The mono-energetic neutron energies of 1.12-4.12 MeV were generated from the {sup 7}Li(p,n) reaction by using the proton energies of 3 and 4 MeV from the folded tandem ion beam accelerator (FOTIA) at BARC as well as 5 and 6 MeV from the Pelletron facility at TIFR, Mumbai. The {sup 115}In(n,γ){sup 116m}In reaction cross-section was used as the neutron flux monitor. The {sup 197}Au(n,γ){sup 198}Au reaction cross-section at the neutron energies of 3.12 and 4.12 MeV are reported for the first time. The {sup 197}Au(n,γ){sup 198}Au reaction cross-sections at 1.12 and 2.12 MeV are close to the literature data of in between neutron energies. The {sup 197}Au(n,γ){sup 198}Au cross-section was also calculated theoretically by using the computer code TALYS 1.6 and found to be higher than the experimental data of present work and literature data within the neutron energies of 0.8 to 4 MeV.

Effect of styrene maleic acid WIN55,212-2 micelles on neuropathic pain in a rat model.

Science.gov (United States)

Linsell, Oliver; Brownjohn, Philip W; Nehoff, Hayley; Greish, Khaled; Ashton, John C

2015-05-01

Cannabinoid receptor agonists are moderately effective at reducing neuropathic pain but are limited by psychoactivity. We developed a styrene maleic acid (SMA) based on the cannabinoid WIN 55,212-2 (WIN) and tested in a rat model of neuropathic pain and in the rotarod test. We hypothesized that miceller preparation can ensure prolonged plasma half-life being above the renal threshold of excretion. Furthermore, SMA-WIN could potentially reduce the central nervous system effects of encapsulated WIN by limiting its transport across the blood-brain barrier. Using the chronic constriction injury model of sciatic neuropathy, the SMA-WIN micelles were efficacious in the treatment of neuropathic pain for a prolonged period compared to control (base WIN). Attenuation of chronic constriction injury-induced mechanical allodynia occurred for up to 8 h at a dose of 11.5 mg/kg of SMA-WIN micelles. To evaluate central effects on motor function, the rotarod assessment was utilized. Results showed initial impairment caused by SMA-WIN micelles to be identical to WIN control for up to 1.5 h. Despite this, the SMA-WIN micelle formulation was able to produce prolonged analgesia over a time when there was decreased impairment in the rotarod test compared with base WIN.

Astatine-211 labelled proteins and their stability in vivo

International Nuclear Information System (INIS)

Yi Changhou; Jin Jannan; Zhang Shuyuan; Wang Ketai; Zhang Dayuan; Zhou Maolun

1989-01-01

211 At or 131 I labelled proteins, e.g. 211 At-IgG or 211 At-BSA (bovine serum albumin) were prepared by 211 At reaction with the diazo-compound of para-aminobenzoic acid, which is then conjugated with IgG or BSA via an acylation reaction. The 211 At-carbon bond was found metabolically stable under in vivo conditions. For the labelling of proteins with 211 At or 131 I, other methods of direct oxidation are also described. The results show that for the labelling of proteins with 211 At, high rate of incorporation can be obtained with hydrogen peroxide as oxidant, but the labelling of proteins with 131 I is more favourable with the strong oxidant Chloramine-T. (author) 12 refs.; 6 figs

[Impact of personality disorders in a sample of 212 homeless drug users].

Science.gov (United States)

Combaluzier, S; Gouvernet, B; Bernoussi, A

2009-10-01

The impact of the mental disorders (axis I and II, according to DSM IV) on psychosocial problems (axis IV) is now a well-known fact, notably when substance abuse disorders are encountered on axis I. This leads to the conclusion that personality disorders increase the risk of substance abuse, that substance abuse increases the risk of homelessness, that dual diagnosis has a high impact on homelessness and underlines interactions between personality disorders (PD), drug abuse (DA) and homelessness. The aim of this paper is also to study these interactions. We will process the classical epidemiological measures, which have already produced interesting findings on other substance-linked disorders. We will study the multiplicative interaction (I(AB)) and the etiological fraction (EFi) linked to interaction, which evaluate the effects of two factors on another. According to the authors, the I(AB) determines whether the co-occurrence of two risk factors in a group induces more cases than each factor acting together; also if the I(AB) is greater than 1 it is possible to estimate the EFi, that proportionally measures the number of cases of the third factor that can be attributed to the co-occurrence. We will also study the interactions of homelessness and PD on DA, of the PD and DA on homelessness, and of this association DA and homelessness on PD. The data we will use in the paper deal with the prevalence of PD in general, drug users (n=226), homeless (n=999) and homeless drug abusers (n=212). The two last data are extracted from the same population and have been collected through clinical interviews, and the diagnosis follows the DSM criteria. They are comparable to Kokkevi et al.'s sample regarding the drug (heroin), the mean age (28 years for Kokkevi et al., 27 years in our sample), and the geographic origin of the populations (Mediterranean basin). The repartition of PD differs significantly (0.001) in the homeless population and the homeless drug abusers (chi(2

13CO2/12CO2 isotope ratio analysis in human breath using a 2 μm diode laser

Science.gov (United States)

Sun, Mingguo; Cao, Zhensong; Liu, Kun; Wang, Guishi; Tan, Tu; Gao, Xiaoming; Chen, Weidong; Yinbo, Huang; Ruizhong, Rao

2015-04-01

The bacterium H. pylori is believed to cause peptic ulcer. H. pylori infection in the human stomach can be diagnosed through a CO2 isotope ratio measure in exhaled breath. A laser spectrometer based on a distributed-feedback semiconductor diode laser at 2 μm is developed to measure the changes of 13CO2/12CO2 isotope ratio in exhaled breath sample with the CO2 concentration of ~4%. It is characterized by a simplified optical layout, in which a single detector and associated electronics are used to probe CO2 spectrum. A new type multi-passes cell with 12 cm long base length , 29 m optical path length in total and 280 cm3 volume is used in this work. The temperature and pressure are well controlled at 301.15 K and 6.66 kPa with fluctuation amplitude of 25 mK and 6.7 Pa, respectively. The best 13δ precision of 0.06o was achieved by using wavelet denoising and Kalman filter. The application of denoising and Kalman filter not only improved the signal to noise ratio, but also shorten the system response time.

Structure of 12B from measurement and R-matrix analysis of sigma(theta) for 11B(n,n)11B and 11B(n,n')11Bsup(*)(2.12 MeV), and shell-model calculations

International Nuclear Information System (INIS)

Koehler, P.E.; Knox, H.D.; Resler, D.A.; Lane, R.O.

1983-01-01

Differential cross sections for neutrons, elastically scattered from 11 B and inelastically scattered to the first excited state 11 B*(2.12 MeV) have been measured at 13 incident energies for 4.8 12 B of 7.8 to 10.3 MeV. The cross sections were measured at nine laboratory angles per energy from 20 0 to 160 0 and show considerable resonance structure. Differential inelastic cross sections were also measured for the 4.45 and 5.02 MeV levels of 11 B for 2 to 9 angles at several incident energies. These new elastic and inelastic 2.12 MeV level data have been analyzed together with previously publsihed cross sections for 2 12 B. The shell model was used to calculate states in 12 B as well as spectroscopic amplitudes for reactions leading to these states. The results of this model calculation are compared to those of the R-matrix analysis. Much of the structure observed in the experimental work is predicted by the model for Esub(x) < or approx. 7 MeV. For levels of higher excitation the agreement is not as good. The experimental data are also compared to continuum shell-model calculations. (orig.)

A 100 au Wide Bipolar Rotating Shell Emanating from the HH 212 Protostellar Disk: A Disk Wind?

Science.gov (United States)

Lee, Chin-Fei; Li, Zhi-Yun; Codella, Claudio; Ho, Paul T. P.; Podio, Linda; Hirano, Naomi; Shang, Hsien; Turner, Neal J.; Zhang, Qizhou

2018-03-01

HH 212 is a Class 0 protostellar system found to host a “hamburger”-shaped dusty disk with a rotating disk atmosphere and a collimated SiO jet at a distance of ∼400 pc. Recently, a compact rotating outflow has been detected in SO and SO2 toward the center along the jet axis at ∼52 au (0.″13) resolution. Here we resolve the compact outflow into a small-scale wide-opening rotating outflow shell and a collimated jet, with the observations in the same S-bearing molecules at ∼16 au (0.″04) resolution. The collimated jet is aligned with the SiO jet, tracing the shock interactions in the jet. The wide-opening outflow shell is seen extending out from the inner disk around the SiO jet and has a width of ∼100 au. It is not only expanding away from the center, but also rotating around the jet axis. The specific angular momentum of the outflow shell is ∼40 au km s‑1. Simple modeling of the observed kinematics suggests that the rotating outflow shell can trace either a disk wind or disk material pushed away by an unseen wind from the inner disk or protostar. We also resolve the disk atmosphere in the same S-bearing molecules, confirming the Keplerian rotation there.

Evaluación del efecto del agonista cannabinoide WIN 55,212-2 en el tratamiento de los efectos secundarios producidos por el fármaco antineoplásico 5-fluorouracilo en rata

OpenAIRE

Muñoz Huertas, María

2016-01-01

Los efectos secundarios producidos por la quimioterapia anticancerosa suponen un problema para el que se han propuesto los cannabinoides como tratamiento (a pesar de su principal limitación, los efectos centrales). El antineoplásico 5-Fluorouracilo (5-FU) produce en los pacientes náuseas, vómitos, diarrea, neuropatía periférica y, posiblemente, dolor visceral. Se evaluó si el agonista cannabinoide WIN 55, 212-2 (WIN), a dosis no psicoactivas, puede ser efectivo para tratar dichos efectos secu...

Puberty in perinatal HIV-1 infection: a multicentre longitudinal study of 212 children.

Science.gov (United States)

de Martino, M; Tovo, P A; Galli, L; Gabiano, C; Chiarelli, F; Zappa, M; Gattinara, G C; Bassetti, D; Giacomet, V; Chiappini, E; Duse, M; Garetto, S; Caselli, D

2001-08-17

To define age at entry into Tanner stages in children with perinatal HIV-1 infection. Multicentre longitudinal study including 212 perinatally HIV-1-infected children (107 girls and 105 boys) followed-up during puberty (from 8 and 9 years onwards in girls and boys, respectively). Healthy children (843 girls and 821 boys) provided reference percentiles. P2 or B2 stages in girls and P2 or G2 stages in boys defined onset of puberty. The cumulative probability [95% confidence limit (CI)] of entry into each stage at different ages was estimated by the Kaplan-Meier product-limit method; differences were evaluated by log rank test. Relationships were tested using the Spearman's rank correlation coefficient. Ages of girls [years (95%CI)] at P2 [12.9 (12.6-13.2)], P3 [13.4 (13.0-13.8)], P4 [14.6 (14.0-15.2)], B2 [12.7 (12.2-13.2)], B3 [13.3 (12.8-14.0)] and B4 [14.6 (14.0-15.2)] stages were > 97th percentile (> or = 21 month delay) of controls. Ages of boys [years (95%CI)] at P2 [12.6 (12.1-13.1)], P3 [13.9 (13.4-14.4)], P4 [14.9 (14.2-15.6)], G2 [12.1 (11.5-12.7)], G3 [13.6 (13.1-14.1)] and G4 [14.9 (14.1-15.7)] stages were at the 75-97th percentiles (< or = 15 month delay). Age at onset of puberty was not related to clinical and immunological condition, antiretroviral treatment, weigh for height and age at onset of severe disease or immune suppression. Perinatal HIV-1 infection interferes with sexual maturation. The mechanisms by which this occurs should be elucidated and intervention strategies designed. Intervention could save much psychological distress, since associated linear growth failure can exacerbate adolescents' feelings of being different and unwell.

Human radiation studies: Remembering the early years: Oral history of Dr. Patricia Wallace Durbin, Ph.D., conducted November 11, 1994

International Nuclear Information System (INIS)

1995-07-01

This report is a transcript of an interview of Dr. Patricia Wallace Durbin by representatives of the US DOE Office of Human Radiation Research. Dr. Durbin was selected for this interview because of her knowledge of the human plutonium injections and her recollections of key figures, especially Joseph Hamilton. After a brief biographical sketch Dr. Durbin discusses her loss of research funding from DOE, her recollections concerning research into strontium metabolism as part of Project Sunshine, her recollections relating to the rationale for studies of human metabolism of radionuclides, her remembrances of Dr. Hamilton's Astatine and Plutonium research, and her experiences in gathering archival records concerning these researches

Acinetobacter baumannii isolate BAL_212 from Vietnam produces the K57 capsular polysaccharide containing a rarely occurring amino sugar N-acetylviosamine.

Science.gov (United States)

Kenyon, Johanna J; Kasimova, Anastasiya A; Shashkov, Alexander S; Hall, Ruth M; Knirel, Yuriy A

2018-02-01

The structures of capsular polysaccharides (CPSs) produced by different Acinetobacter baumannii strains have proven to be invaluable in confirming the role of specific genes in the synthesis of rare sugars through the correlation of genetic content at the CPS biosynthesis locus with sugars found in corresponding CPS structures. A module of four genes (rmlA, rmlB, vioA and vioB) was identified in the KL57 capsule biosynthesis gene cluster of A. baumannii isolate BAL_212 from Vietnam. These genes were predicted to direct the synthesis of 4-acetamido-4,6-dideoxy-d-glucose (N-acetylviosamine, d-Qui4NAc) and the K57 CPS was found to contain this monosaccharide. The K57 structure was determined and, in addition to d-Qui4NAc, included three N-acetylgalactosamine residues in the main chain, with a single glucose side branch. The KL57 gene cluster has not been found in any other A. baumannii genomes, but the rmlA-rmlB-vioA-vioB module is present in the KL119 gene cluster that would likely produce a d-Qui4NAc-containing CPS.

Investigations of the tracheobronchial epithelium of rat after X-ray irradiation and inhalation of 212Pb aerosol

International Nuclear Information System (INIS)

Petri, P.

1981-01-01

Early reactions of the tracheobronchial epithelium of rats. Be up to 96 h after irradiation have been investigated. Detection of autoradiographically labelled DNA in the basal cells is a measure of the regenerative function of the epithelium. The labelling index is determined on the basis of histological preparations of trachea and bronchi. Each group of animals was exposed to partial irradiation of the thorax of 500 R, 1000 R and 1500 R. A dose-dependent reduction of the labelling index is found with a minimum after 24 h. A further group of animals inhaled 212 Pb aerosol while the control group was given inactive aerosol. The calculations of Hofmann (1969) yield a value of about 170 rad for the trachea and 480 rad for the upper part of the lungs. The labelling index after 12 h is lower than in the animals exposed to 500 R. It is significantly higher in the lobar bronchi. At the time of sacrificing, the labelling index is higher in all regions than the labelling index of 500 R animals. This labelling method enables quantitative determination of DNA synthesis and labelling index after radiation exposure. The study did not indicate the stage of development in which the so-called ''replacement cells'' of the bronchial epithelium are influenced by radiation exposure. Radionuclide inhalation does not affect the bronchial DNA synthesis index as strongly as assumed on the basis of dose estimates. Ater 12 h, the trachea shows a stronger reaction than the bronchi. Explanations are offered. (orig./MG) [de

Why is the neutrino oscillation formula expanded in Δm_2_1"2/Δm_3_1"2 still accurate near the solar resonance in matter?

International Nuclear Information System (INIS)

Xu, Xun-Jie

2015-01-01

The conventional approximate formula for neutrino oscillation in matter which is obtained from the expansion in terms of the ratio of mass square differences α=Δm_2_1"2/Δm_3_1"2≈0.03, first proposed by Cervera, et al. and Freund, turns out to be an accurate formula for accelerator neutrino experiments. Originally it required the neutrino energy to be well above the solar resonance to validate the expansion but it is found to be still very accurate when the formula is extrapolated to the resonance, which is practically important for the T2K experiment. This paper shows that the accuracy is guaranteed by cancellations of branch cut singularities and also, for the first time, analytically computes the actual error of the formula. The actual error implies that the original requirement can be safely removed in current experiments.

A randomized, multicentre study of directed daylight exposure times of 11/2 vs. 21/2 h in daylight-mediated photodynamic therapy with methyl aminolaevulinate in patients with multiple thin actinic keratoses of the face and scalp

DEFF Research Database (Denmark)

Wiegell, S.R.; Fabricius, S.; Philipsen, P.A.

2011-01-01

, time of day or time of year during which the treatment was performed. Treatment was well tolerated, with a mean ± SD maximal pain score of 1·3 ± 1·5. Conclusions: Daylight-mediated MAL-PDT is an effective, convenient and nearly pain-free treatment for patients with multiple thin AKs. Daylight-mediated......, the inconvenience of clinic attendance and discomfort during therapy are significant drawbacks. Daylight-mediated PDT could potentially reduce these and may serve as an alternative to conventional PDT. Objectives: To compare the efficacy of methyl aminolaevulinate (MAL)-PDT with 11/2 vs. 21/2 h of daylight exposure...

TMEM106B, the risk gene for frontotemporal dementia, is regulated by the miRNA-132/212 cluster and affects progranulin pathways

Science.gov (United States)

Chen-Plotkin, Alice S.; Unger, Travis L.; Gallagher, Michael D.; Bill, Emily; Kwong, Linda K.; Volpicelli-Daley, Laura; Busch, Johanna I.; Akle, Sebastian; Grossman, Murray; Van Deerlin, Vivianna; Trojanowski, John Q.; Lee, Virginia M.-Y.

2012-01-01

Frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP) is a fatal neurodegenerative disease with no available treatments. Mutations in the progranulin gene (GRN) causing impaired production or secretion of progranulin are a common Mendelian cause of FTLD-TDP; additionally, common variants at chromosome 7p21 in the uncharacterized gene TMEM106B were recently linked by genome-wide association to FTLD-TDP with and without GRN mutations. Here we show that TMEM106B is neuronally expressed in postmortem human brain tissue, and that expression levels are increased in FTLD-TDP brain. Furthermore, using an unbiased, microarray-based screen of over 800 microRNAs, we identify microRNA-132 as the top microRNA differentiating FTLD-TDP and control brains, with progranulin in late endo-lysosomes, and TMEM106B over-expression increases intracellular levels of progranulin. Thus, TMEM106B is an FTLD-TDP risk gene, with microRNA-132/212 depression as an event which can lead to aberrant over-expression of TMEM106B, which in turn alters progranulin pathways. Evidence for this pathogenic cascade includes the striking convergence of two independent, genomic-scale screens on a microRNA:mRNA regulatory pair. Our findings open novel directions for elucidating miRNA-based therapies in FTLD-TDP. PMID:22895706

[Family paracentric inversion of the short arm of chromosome X (Xp21.2p11.23) and connection with autism spectrum disorders].

Science.gov (United States)

Milovančević, Milica Pejović; Vešić, Marija; Jelisavčić, Marko; Nikšić, Snežana; Pilić, Gordana Radivojević; Maravić, Vanja Mandić

2012-01-01

Autism spectrum disorders (ASDs) are a group of complex pervasive developmental disorders characterized by impairments in communication, social interaction and behavior. In most cases autism is caused by a combination of genetic factors and environmental risk factors. In 10% to 20% of cases it has been shown that the cause of ASD is genetic. We are describing a 2-year-old boy who was referred to genetic counseling because of speech delay and certain autism-like behavior. By cytogenetic analysis the karyotype 46, inv(X),Y was obtained. The boy was a carrier of a paracentric inversion of the short arm of the chromosome X. After cytogenetic analysis of parental blood, it was detected that mother was a carrier of identical aberration, but had no clinical signs. The method of fluorescent in situ hybridization (FISH) yielded the precise breakpoint in the region (p21.2p11.23). Mother and son were carriers of identical X chromosome. Breakpoints are located in the regions that have already been linked to autism, which indicates that the positional effect of the gene could have been a possible cause of the patient's genotype. In addition to positional effects, in order to better understand the etiology of autism other genetic and environmental factors should be always taken into consideration.

Microdosimetry of astatine-211 and comparison with that of iodine-125

International Nuclear Information System (INIS)

Unak, T.

2001-01-01

211 At is an alpha and Auger emitter radionuclide and has been frequently used for labeling of different kind of chemical agents. 125 I is also known as an effective Auger emitter. The radionuclides which emit short range and high LET radiations such as alpha particles and Auger electrons have high radiotoxic effectiveness on the living systems. The microdosimetric data are suitable to clarify the real radiotoxic effectiveness and to get the detail of diagnostic and therapeutic application principles of these radionuclides. In this study, the energy and dose absorptions by cell nucleus from alpha particles and Auger electrons emitted by 211 At have been calculated using a Monte Carlo calculation program (code: UNMOC). For these calculations two different model corresponding to the cell nucleus have been used and the data obtained were compared with the data earlier obtained for 125 I. As a result, the radiotoxicity of 211 At is in the competition with 125 I. In the case of a specific agent labelled with 211 At or 125 I is incorporated into the cell or cell nucleus, but non-bound to DNA or not found very close to it, 211 At should considerably be much more radiotoxic than 125 I, but in the case of the labelled agent is bound to DNA or take a place very close to it, the radiotoxicity of 125 I should considerably be higher than 211 At. (author)

Targeted radionuclide therapy with astatine-211: Oxidative dehalogenation of astatobenzoate conjugates.

Science.gov (United States)

Teze, David; Sergentu, Dumitru-Claudiu; Kalichuk, Valentina; Barbet, Jacques; Deniaud, David; Galland, Nicolas; Maurice, Rémi; Montavon, Gilles

2017-05-31

211 At is a most promising radionuclide for targeted alpha therapy. However, its limited availability and poorly known basic chemistry hamper its use. Based on the analogy with iodine, labelling is performed via astatobenzoate conjugates, but in vivo deastatination occurs, particularly when the conjugates are internalized in cells. Actually, the chemical or biological mechanism responsible for deastatination is unknown. In this work, we show that the C-At "organometalloid" bond can be cleaved by oxidative dehalogenation induced by oxidants such as permanganates, peroxides or hydroxyl radicals. Quantum mechanical calculations demonstrate that astatobenzoates are more sensitive to oxidation than iodobenzoates, and the oxidative deastatination rate is estimated to be about 6 × 10 6 faster at 37 °C than the oxidative deiodination one. Therefore, we attribute the "internal" deastatination mechanism to oxidative dehalogenation in biological compartments, in particular lysosomes.

Family paracentric inversion of the short arm of chromosome X (Xp21.2p11.23 and connection with autism spectrum disorders

Directory of Open Access Journals (Sweden)

Pejović-Milovančević Milica

2012-01-01

Full Text Available Introduction. Autism spectrum disorders (ASDs are a group of complex pervasive developmental disorders characterized by impairments in communication, social interaction and behavior. In most cases autism is caused by a combination of genetic factors and environmental risk factors. In 10% to 20% of cases it has been shown that the cause of ASD is genetic. Case Outline. We are describing a 2-year-old boy who was referred to genetic counseling because of speech delay and certain autism-like behavior. By cytogenetic analysis the karyotype 46, inv(X,Y was obtained. The boy was a carrier of a paracentric inversion of the short arm of the chromosome X. After cytogenetic analysis of parental blood, it was detected that mother was a carrier of identical aberration, but had no clinical signs. The method of fluorescent in situ hybridization (FISH yielded the precise breakpoint in the region (p21.2p11.23. Mother and son were carriers of identical X chromosome. Conclusion. Breakpoints are located in the regions that have already been linked to autism, which indicates that the positional effect of the gene could have been a possible cause of the patient’s genotype. In addition to positional effects, in order to better understand the etiology of autism other genetic and environmental factors should be always taken into consideration. [Projekat Ministarstva nauke Republike Srbije, br. ON175013

Differential effects of repeated low dose treatment with the cannabinoid agonist WIN 55,212-2 in experimental models of bone cancer pain and neuropathic pain

DEFF Research Database (Denmark)

Hald, Andreas; Ding, Ming; Egerod, Kristoffer Lihme

2008-01-01

Pain due to bone malignancies is one of the most difficult types of cancer pain to fully control and may further decrease the patients' quality of life. Animal models of chronic pain conditions resulting from peripheral inflammatory reactions or nerve injuries are responsive to treatment with can......Pain due to bone malignancies is one of the most difficult types of cancer pain to fully control and may further decrease the patients' quality of life. Animal models of chronic pain conditions resulting from peripheral inflammatory reactions or nerve injuries are responsive to treatment...... with cannabinoid agonists. However, the use of cannabinoid agonists in humans may be hampered by CNS related side effects and development of tolerance. In the present study, we investigated the effect of repeated low dose administration of the synthetic cannabinoid agonist WIN 55,212-2 on bone cancer pain...... and neuropathic pain in mice. In addition, we investigated the development of CNS related side effects and tolerance. We found that 0.5 mg/kg/day for 18 days reduced pain related behavior and expression of spinal glial fibrillary acidic protein in the bone cancer pain model but not in the neuropathic pain model...

212__Omoniyi-LOKOJA

African Journals Online (AJOL)

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environmental laws that borders on proper solid waste and effluent disposals. Key Words: Heavy .... Samples Pre - Treatment. Soil samples from .... bioavailability to plants (Oluyemi et al., 2008). 207 ..... M.Sc. Thesis, Department of. Chemistry ...

Time intervals from first symptom to treatment of cancer: a cohort study of 2,212 newly diagnosed cancer patients

Directory of Open Access Journals (Sweden)

Sokolowski Ineta

2011-10-01

Full Text Available Abstract Background Delay in diagnosis of cancer may worsen prognosis. The aim of this study is to explore patient-, general practitioner (GP- and system-related delay in the interval from first cancer symptom to diagnosis and treatment, and to analyse the extent to which delays differ by cancer type. Methods Population-based cohort study conducted in 2004-05 in the County of Aarhus, Denmark (640,000 inhabitants. Data were collected from administrative registries and questionnaires completed by GPs on 2,212 cancer patients newly diagnosed during a 1-year period. Median delay (in days with interquartile interval (IQI was the main outcome measure. Results Median total delay was 98 days (IQI 57-168. Most of the total delay stemmed from patient (median 21 days (7-56 and system delay (median 55 days (32-93. Median GP delay was 0 (0-2 days. Total delay was shortest among patients with ovarian (median 60 days (45-112 and breast cancer (median 65 days (39-106 and longest among patients with prostate (median 130 days (89-254 and bladder cancer (median 134 days (93-181. Conclusion System delay accounted for a substantial part of the total delay experienced by cancer patients. This points to a need for shortening clinical pathways if possible. A long patient delay calls for research into patient awareness of cancer. For all delay components, special focus should be given to the 4th quartile of patients with the longest time intervals and we need research into the quality of the diagnostic work-up process. We found large variations in delay for different types of cancer. Improvements should therefore target both the population at large and the specific needs associated with individual cancer types and their symptoms.

Use of interhalogen exchange for preparation of astatine-benzene and iodo-benzene

International Nuclear Information System (INIS)

Kolachkovski, A.; Khalkin, V.A.

1976-01-01

Experimental testing of interhalogen exchange between the solid and liquid phases has been carried out at 155 deg C with particular reference to a NaOH-Na 131 I-BrPh system. Iodine transition rate is dependent on the process duration and alkali amount. The relative amounts of 131 IPh resultant from the reaction of interhalogen exchange is evaluated by paper chromatography. The results obtained may be considered as those which provide experimental support for the assumed efficiency of the production of 131 IPh based on the reaction of interhalogen exchange to yield 70-80% 131 IPh

8 CFR 212.4 - Applications for the exercise of discretion under section 212(d)(1) and 212(d)(3).

Science.gov (United States)

2010-01-01

... SECURITY IMMIGRATION REGULATIONS DOCUMENTARY REQUIREMENTS: NONIMMIGRANTS; WAIVERS; ADMISSION OF CERTAIN... Card, issued by the DOS shall be valid for a period not to exceed the validity of the biometric BCC for... is noted on the card may remain valid. Although the waiver may remain valid, the non-biometric border...

Chemical concentration of a new natural spontaneously fissionable nuclide from solutions with low salt background

International Nuclear Information System (INIS)

Korotkin, Yu.S.; Ter-Akop'yan, G.M.; Popeko, A.G.; Drobina, T.P.; Zhuravleva, E.L.

1982-01-01

The results of experiments on further concentration of a new natural spontaneously fissionable nuclide, the concentrates of which form the Cheleken geothermal brines have been obtained, are presented. The conclusions are drown about the chemical nature of a new spontaneously fissionable nuclide. It is a chalcophile element which copreipitates with sulphides of copper, lead, arsenic and mercury from weakly acid solutions. The behaviour of the new nuclide in sulphide systems in many respects is similar to the behaviour of polonium, astatine and probably of bismuth. The most probable stable valence of the new nuclide varies from +1 up to +3. The data available on the chemical behaviour of the new nuclide as well as the analysis over contamination by spontaneously fissionable isotopes permit to state that the new natural spontaneously fissionable nuclide does not relate to the known isotopes

Is Electronegativity a Useful Descriptor for the 'Pseudo-Alkali-Metal' NH4?

International Nuclear Information System (INIS)

Whiteside, Alexander; Xantheas, Sotiris S.; Gutowski, Maciej S.

2011-01-01

Molecular ions in the form of 'pseudo-atoms' are common structural motifs in chemistry, with properties that are transferrable between different compounds. We have determined the electronegativity of the 'pseudo-alkali metal' ammonium (NH4) and evaluated its reliability as a descriptor in comparison to the electronegativities of the alkali metals. The computed properties of its binary complexes with astatine and of selected borohydrides confirm the similarity of NH4 to the alkali metal atoms, although the electronegativity of NH4 is relatively large in comparison to its cationic radius. We paid particular attention to the molecular properties of ammonium (angular anisotropy, geometric relaxation, and reactivity), which can cause deviations from the behaviour expected of a conceptual 'true alkali metal' with this electronegativity. These deviations allow for the discrimination of effects associated with the polyatomic nature of NH4.

Dicty_cDB: [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available VF (Link to library) VFC212 (Link to dictyBase) - - - Contig-U16455-1 VFC212P (Link... to Original site) VFC212F 260 VFC212Z 343 VFC212P 603 - - Show VFC212 Library VF (Link to library) Clone ID VFC212 (Link to dict...yBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig Contig-U16455-1 Original site URL http://dict...actor 1 alpha. 394 0.0 3 X55972 |X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha. 347 0.0 3...4.0 %: vesicles of secretory system 4.0 %: endoplasmic reticulum >> prediction fo

Measurement of the 115In(n,γ)116 m In reaction cross-section at the neutron energies of 1.12, 2.12, 3.12 and 4.12 MeV

Science.gov (United States)

Lawriniang, Bioletty Mary; Badwar, Sylvia; Ghosh, Reetuparna; Jyrwa, Betylda; Vansola, Vibha; Naik, Haladhara; Goswami, Ashok; Naik, Yeshwant; Datrik, Chandra Shekhar; Gupta, Amit Kumar; Singh, Vijay Pal; Pol, Sudir Shibaji; Subramanyam, Nagaraju Balabenkata; Agarwal, Arun; Singh, Pitambar

2015-08-01

The 115In(n,γ)116 m In reaction cross section at neutron energies of 1.12, 2.12, 3.12 and 4.12 MeV was determined by using an activation and off-line γ-ray spectrometric technique. The monoenergetic neutron energies of 1.12 - 4.12 MeV were generated from the 7Li(p,n) reaction by using proton beam with energies of 3 and 4 MeV from the folded tandem ion beam accelerator (FOTIA) at Bhabha Atomic Research Centre (BARC) and with energies of 5 and 6 MeV from the Pelletron facility at Tata Institute of Fundamental Research (TIFR), Mumbai. The 197Au(n,γ)198Au reaction cross-section was used as the neutron flux monitor.The 115In(n,γ)116 m In reaction cross section at neutron energies of 1.12, 2.12, 3.12 and 4.12 MeV was determined by using an activation and off-line γ-ray spectrometric technique. The monoenergetic neutron energies of 1.12 - 4.12 MeV were generated from the 7Li(p,n) reaction by using proton beam with energies of 3 and 4 MeV from the folded tandem ion beam accelerator (FOTIA) at Bhabha Atomic Research Centre (BARC) and with energies of 5 and 6 MeV from the Pelletron facility at Tata Institute of Fundamental Research (TIFR), Mumbai. The 197Au(n,γ)198 Au reaction cross-section was used as the neutron flux monitor. The 115In(n,γ)116 m In reaction cross-sections at neutron energies of 1.12 - 4.12 MeV were compared with the literature data and were found to be in good agreement with one set of data, but not with others. The 115In(n,γ)116 m In cross-section was also calculated theoretically by using the computer code TALYS 1.6 and was found to be slightly lower than the experimental data from the present work and the literature.)198Au reaction cross-section was used as the neutron flux monitor. The 115In(n,γ)116 m In reaction cross-sections at neutron energies of 1.12 - 4.12 MeV were compared with the literature data and were found to be in good agreement with one set of data, but not with others. The 115In(n,γ)116 m In cross-section was also calculated

The synthesis of SL-75.212 (Betaxolol) labelled with carbon 14: 1-[4-(2-cyclopropyl methoxyethyl-[1-14C]) phenoxy]-3-isopropyl amino-2-propanol

International Nuclear Information System (INIS)

Aubert, F.; Beaucourt, J.P.; Pichat, L.

1982-01-01

Carbonation with 14 CO 2 of the Grigard reagent 1 gave 4-benzyloxy [carboxyl- 14 C] benzoic acid: 2 (87 % yield). 2 was successively treated in diethyl ether solution with diazomethane and lithium aluminium hydride giving rise to [7- 14 C] 4 benzyloxybenzyl alcohol 4 (82 % yield). Alcohol 4 was transformed into the corresponding chloride 5 when exposed to thionylchloride in ether. 5 was condensed with NaCN in DMF to give the nitrile 6 which was hydrolysed into the acid 7 isolated in a 75 % overall yield from Ba 14 CO 3 . 7 gave the alcohol 9 by successive treatments with diazomethane and LiAlH 4 in ether. 9 with NaH gave the corresponding alkoxide which when condensed with bromomethylcyclopropane gave the ether 10 purified by silicagel column chromatography and isolated with an overall yield of 71 % from Ba 14 CO 3 . Hydrogenolysis of 10 gave the phenol 11. The epoxide 12 was secured by condensation with epichlorhydrin in presence of NaOH. After purification by silicagel column chromatography 10 was opened with isopropylamine leading to the target compound BETAXOLOL 13 isolated as the hydrochloride. After extensive purification by Sephadex G-10 column chromatography, SL 75.212 [ethyl-1- 14 C] was obtained in an overall yield of 26 % from barium [ 14 C] carbonate and a radiochemical purity better than 99 % (specific activity 57 mCi/mole). (author)

Glomerular filtration rate after alpha-radioimmunotherapy with 211At-MX35-F(ab')2: a long-term study of renal function in nude mice

DEFF Research Database (Denmark)

Back, T.; Haraldsson, B.; Hultborn, R

2009-01-01

of the glomerular filtration rate (GFR). The renal toxicity was evaluated at levels close to the dose limit for the bone marrow and well within the range for therapeutic efficacy on tumors. Astatinated MX35-F(ab')(2) monoclonal antibodies were administered intravenously to nude mice. Both non-tumor-bearing animals...... manifested late. Examination of the kidney sections showed histologic changes that were overall subdued. Following alpha-RIT with (211)At-MX35-F(ab')(2) at levels close to the dose limit of severe myelotoxicity, the effects found on renal function were relatively small, with only minor to moderate reductions...... in GFR. These results suggest that a mean absorbed dose to the kidneys of approximately 10 Gy is acceptable, and that the kidneys would not be the primary dose-limiting organ in systemic alpha-RIT when using (211)At-MX35-F(ab')(2) Udgivelsesdato: 2009/12...

Boiling points of the superheavy elements 117 and 118

International Nuclear Information System (INIS)

Takahashi, N.

2001-01-01

It has been shown that the relativistic effect on the electrons reveal in the heavy element region. What kind of changes will appear in the heavy elements because of the relativistic effects? Can we observe the changes? We observed that the boiling points of astatine and radon are lower than that extrapolated values from lighter elements in the same groups. Systematic behavior of the elements on the boiling point was examined and a new method for the estimation of the boiling points of the superheavy elements in the halogen and rare gases has been found. The estimated values of the elements 117 and 118 are 618 and 247 K, respectively which are considerably lower than those obtained until now. If these values are correct the production of the superheavy elements with heavy ions reaction may be affected. Further, the chemical properties may be fairly different from the lighter elements. (author)

Alpha particle induced DNA damage and repair in normal cultured thyrocytes of different proliferation status

DEFF Research Database (Denmark)

Lyckesvärd, Madeleine Nordén; Delle, Ulla; Kahu, Helena

2014-01-01

Childhood exposure to ionizing radiation increases the risk of developing thyroid cancer later in life and this is suggested to be due to higher proliferation of the young thyroid. The interest of using high-LET alpha particles from Astatine-211 ((211)At), concentrated in the thyroid by the same...... mechanism as (131)I [1], in cancer treatment has increased during recent years because of its high efficiency in inducing biological damage and beneficial dose distribution when compared to low-LET radiation. Most knowledge of the DNA damage response in thyroid is from studies using low-LET irradiation...... and much less is known of high-LET irradiation. In this paper we investigated the DNA damage response and biological consequences to photons from Cobolt-60 ((60)Co) and alpha particles from (211)At in normal primary thyrocytes of different cell cycle status. For both radiation qualities the intensity...

Final Report for grant entitled "Production of Astatine-211 for U.S. Investigators"

Energy Technology Data Exchange (ETDEWEB)

Wilbur, Daniel Scott

2012-12-12

Alpha-particle emitting radionuclides hold great promise in the therapy of cancer, but few alpha-emitters are available to investigators to evaluate. Of the alpha-emitters that have properties amenable for use in humans, 211At is of particular interest as it does not have alpha-emitting daughter radionuclides. Thus, there is a high interest in having a source of 211At for sale to investigators in the US. Production of 211At is accomplished on a cyclotron using an alpha-particle beam irradiation of bismuth metal. Unfortunately, there are few cyclotrons available that can produce an alpha particle beam for that production. The University of Washington has a cyclotron, one of three in the U.S., that is currently producing 211At. In the proposed studies, the things necessary for production and shipment of 211At to other investigators will be put into place at UW. Of major importance is the efficient production and isolation of 211At in a form that can be readily used by other investigators. In the studies, production of 211At on the UW cyclotron will be optimized by determining the best beam energy and the highest beam current to maximize 211At production. As it would be very difficult for most investigators to isolate the 211At from the irradiated target, the 211At-isolation process will be optimized and automated to more safely and efficiently obtain the 211At for shipment. Additional tasks to make the 211At available for distribution include obtaining appropriate shipping vials and containers, putting into place the requisite standard operating procedures for Radiation Safety compliance at the levels of 211At activity to be produced / shipped, and working with the Department of Energy, Isotope Development and Production for Research and Applications Program, to take orders, make shipments and be reimbursed for costs of production and shipment.

The potential of 211Astatine for NIS-mediated radionuclide therapy in prostate cancer

International Nuclear Information System (INIS)

Willhauck, Michael J.; Sharif Samani, Bibi-Rana; Goeke, Burkhard; Wolf, Ingo; Senekowitsch-Schmidtke, Reingard; Stark, Hans-Juergen; Meyer, Geerd J.; Knapp, Wolfram H.; Morris, John C.; Spitzweg, Christine

2008-01-01

We reported recently the induction of selective iodide uptake in prostate cancer cells (LNCaP) by prostate-specific antigen (PSA) promoter-directed sodium iodide symporter (NIS) expression that allowed a significant therapeutic effect of 131 I. In the current study, we studied the potential of the high-energy alpha-emitter 211 At, also transported by NIS, as an alternative radionuclide after NIS gene transfer in tumors with limited therapeutic efficacy of 131 I due to rapid iodide efflux. We investigated uptake and therapeutic efficacy of 211 At in LNCaP cells stably expressing NIS under the control of the PSA promoter (NP-1) in vitro and in vivo. NP-1 cells concentrated 211 At in a perchlorate-sensitive manner, which allowed a dramatic therapeutic effect in vitro. After intrapertoneal injection of 211 At (1 MBq), NP-1 tumors accumulated approximately 16% ID/g 211 At (effective half-life 4.6 h), which resulted in a tumor-absorbed dose of 1,580 ± 345 mGy/MBq and a significant tumor volume reduction of up to 82 ± 19%, while control tumors continued their growth exponentially. A significant therapeutic effect of 211 At has been demonstrated in prostate cancer after PSA promoter-directed NIS gene transfer in vitro and in vivo suggesting a potential role for 211 At as an attractive alternative radioisotope for NIS-targeted radionuclide therapy, in particular in smaller tumors with limited radionuclide retention time. (orig.)

Astatine-211-labeled biotin conjugates resistant to biotinidase for use in pretargeted radioimmunotherapy

International Nuclear Information System (INIS)

Foulon, Catherine F.; Alston, Kevin L.; Zalutsky, Michael R.

1998-01-01

We report herein the preparation and biological evaluation of two radioastatinated biotin conjugates, (3-[ 211 At]astatobenzoyl)norbiotinamide and ((5-[ 211 At]astato-3-pyridinyl)carbonyl)norbiotinamide. Both conjugates were stable in the presence of human serum and cerebrospinal fluid as well as murine serum, indicating a resistance to degradation to biotinidase. The normal tissue clearance of (3-[ 211 At]astatobenzoyl)norbiotinamide and ((5-[ 211 At]astato-3-pyridinyl)carbonyl)norbiotinamide was rapid, as observed previously with their iodo analogues. Also reported are the first syntheses of N-succinimidyl 5-[ 211 At]astato-3-pyridinecarboxylate and 3-[ 211 At]astatoaniline, two reagents of potential utility for labeling proteins and peptides with 211 At

Effects of WIN 55,212-2 mesylate on the anticonvulsant action of lamotrigine, oxcarbazepine, pregabalin and topiramate against maximal electroshock-induced seizures in mice.

Science.gov (United States)

Luszczki, Jarogniew J; Wlaz, Aleksandra; Karwan, Slawomir; Florek-Luszczki, Magdalena; Czuczwar, Stanislaw J

2013-11-15

The aim of this study was to determine the effect of WIN 55,212-2 mesylate (WIN - a non-selective cannabinoid CB1 and CB2 receptor agonist) on the protective action of four second-generation antiepileptic drugs (lamotrigine, oxcarbazepine, pregabalin and topiramate) in the mouse maximal electroshock seizure model. Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2s stimulus duration) delivered via auricular electrodes. Drug-related adverse effects were ascertained by use of the chimney test (evaluating motor performance), the step-through passive avoidance task (assessing long-term memory) and the grip-strength test (evaluating skeletal muscular strength). Total brain concentrations of antiepileptic drugs were measured by high-pressure liquid chromatography to ascertain any pharmacokinetic contribution to the observed antiseizure effect. Results indicate that WIN (5mg/kg, i.p.) significantly enhanced the anticonvulsant action of lamotrigine (Poxcarbazepine in the maximal electroshock-induced tonic seizure test in mice. Furthermore, none of the investigated combinations of WIN with antiepileptic drugs were associated with any concurrent adverse effects with regards to motor performance, long-term memory or muscular strength. Pharmacokinetic characterization revealed that WIN had no impact on total brain concentrations of lamotrigine, oxcarbazepine, pregabalin and topiramate in mice. These preclinical data would suggest that WIN in combination with lamotrigine, pregabalin and topiramate is associated with beneficial anticonvulsant pharmacodynamic interactions in the maximal electroshock-induced tonic seizure test. © 2013 Published by Elsevier B.V.

The Air Force Nuclear Engineering Center Structural Activation and Integrity Evaluation

Science.gov (United States)

1990-03-01

ASTMA -212 1433 406 SS-304 927 263 Lead 96 39 42/Core Walls 2󈧎...34 * 6𔃼" * 5" ASTMA -212 4699 1333 SS-304 3435 974 Lead 3350 1373 43/Wing Walls 24" * 6𔃼" * 5" ASTMA -212 15622 4430 SS-304 5541 1571 Lead 15683 6425 44...Wing Ceiling 28" * 6𔃼" * 5" ASTMA -212 4809 1364 SS-304 305 87 Lead 970 397 45/Wing Floor 28" * 6𔃼" * 5" ASTMA -212 5174 1467 SS-304 322 91 Lead

Coordination chemistry of the 212Pb/212Bi nuclear transformation: Alpha-emitting radiopharmaceuticals

International Nuclear Information System (INIS)

Parks, N.J.; Harris, W.R.; Keen, C.L.; Cooper, S.R.

1992-07-01

Subdivisions of this project are: (a) the synthesis of prototypical thiolate and dithiocarbamate based hexacoordinate complexes, (b) radiochemical engineering for generation of no-carrier-added lead and bismuth radioelements, (c) the first isolation of bismuth-binding proteins from in vivo studies with cyclotron produced 205/206 Bi tracer, and (d) initial development of transport mechanisms for the intracellular radiobiological study of alpha emitting bismuth, and (e) the initiation of chemical equilibrium studies and biochemical pathways with cyclotron-produced, no-carrier-added, 203 Pb (T 1/2 = 51 hr)

75 FR 41909 - Self-Regulatory Organizations; Municipal Securities Rulemaking Board; Notice of Filing of...

Science.gov (United States)

2010-07-19

... Notices of ten business days after the occurrence of the event; (3) deletes the general materiality... Act Rule 15c2-12. \\6\\ The Rule 15c2-12 Amendment expands the current language of such Rule 15c2-12... reflect the materiality standard changes under the Rule 15c2-12 Amendment and would modify the list of...

Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls.

Science.gov (United States)

Nishida, Yasuhiro; Adachi, Kyoko; Kasai, Hiroaki; Shizuri, Yoshikazu; Shindo, Kazutoshi; Sawabe, Akiyoshi; Komemushi, Sadao; Miki, Wataru; Misawa, Norihiko

2005-08-01

A carotenoid biosynthesis gene cluster mediating the production of 2-hydroxyastaxanthin was isolated from the marine bacterium Brevundimonas sp. strain SD212 by using a common crtI sequence as the probe DNA. A sequence analysis revealed this cluster to contain 12 open reading frames (ORFs), including the 7 known genes, crtW, crtY, crtI, crtB, crtE, idi, and crtZ. The individual ORFs were functionally analyzed by complementation studies using Escherichia coli that accumulated various carotenoid precursors due to the presence of other bacterial crt genes. In addition to functionally identifying the known crt genes, we found that one (ORF11, named crtG) coded for a novel enzyme, carotenoid 2,2'-beta-hydroxylase, which showed intriguingly partial homology with animal sterol-C5-desaturase. When this crtG gene was introduced into E. coli accumulating zeaxanthin and canthaxanthin, the resulting transformants produced their 2-hydroxylated and 2,2'-dihydroxylated products which were structurally novel or rare xanthophylls, as determined by their nuclear magnetic resonance and high-performance liquid chromatography/photodiode array detector/atmospheric pressure chemical ionization mass spectrometry spectral data. The new carotenoid produced was suggested to have a strong inhibitory effect on lipid peroxidation.

New developments of the in-source spectroscopy method at RILIS/ISOLDE

CERN Document Server

Marsh, B A; Imai, N; Seliverstov, M D; Rothe, S; Sels, S; Capponi, L; Rossel, R E; Franchoo, S; Wendt, K; Focker, G J; Kalaninova, Z; Sjoedin, A M; Popescu, L; Nicol, T; Huyse, M; Radulov, D; Atanasov, D; Kesteloot, N; Borgmann, Ch; Cocolios, T E; Lecesne, N; Ghys, L; Pauwels, D; Rapisarda, E; Kreim, S; Liberati, V; Wolf, R N; Andel, B; Schweikhard, L; Lane, J; Derkx, X; Kudryavtsev, Yu; Zemlyanoy, S G; Fedosseev, V N; Lynch, K M; Rosenbusch, M; Van Duppen, P; Lunney, D; Manea, V; Barzakh, A E; Andreyev, A N; Truesdale, V; Flanagan, K T; Molkanov, P L; Koester, U; Van Beveren, C; Wienholtz, F; Goodacre, T Day; Antalic, S; Bastin, B; De Witte, H; Fink, D A; Fedorov, D V

2013-01-01

At the CERN ISOLDE facility, long isotope chains of many elements are produced by proton-induced reactions in target materials such as uranium carbide. The Resonance Ionization Laser Ion Source (RILIS) is an efficient and selective means of ionizing the reaction products to produce an ion beam of a chosen isotope. Coupling the RILIS with modern ion detection techniques enables highly sensitive studies of nuclear properties (spins, electromagnetic moments and charge radii) along an isotope chain, provided that the isotope shifts and hyperfine structure splitting of the atomic transitions can be resolved. At ISOLDE the campaign to measure the systematics of isotopes in the lead region (Pb, Bi, Tl and Po) has been extended to include the gold and astatine isotope chains. Several developments were specifically required for the feasibility of the most recent measurements: new ionization schemes (Po, At); a remote controlled narrow line-width mode of operation for the RILIS Ti:sapphire laser (At, Au, Po); isobar fr...

Studies of Stable Octupole Deformations in the Radium Region

CERN Multimedia

2002-01-01

The purpose of the present project is to locate and identify states in the atomic nuclei possessing stable pearshaped octupole deformation. Such states, formally related to the structures known in molecular physics, manifest themselves as families of parity doublets in odd nuclei.\\\\ \\\\ The best possibilities for observing stable octupole deformations are offered in the Ra-region. Both theoretical calculations and experimental indications support such expectations. Such indications are the non-observation of two-phonon octupole vibrational states in the ISOLDE studies of the even-even radium nuclei, and the reversed sign of the decoupling factor of the ground state band in |2|2|5Ra observed in the single-neutron transfer reactions. In order to establish the predicted strong E1 and E3-transitions between the parity doublets in odd nuclei with stable octupole deformations it is proposed to study conversion electrons in odd-mass francium radium and radon isotopes following the @b-decay of francium and astatine. \\...

Biological toxicity of intracellular radionuclide decay. Part of a coordinated programme on radiation biology of Auger emitters and their therapeutic applications

International Nuclear Information System (INIS)

Hofer, K.G.

1980-06-01

Internal radiotherapy should be performed with short-lived radionuclides which emit high LET radiation and short ranged radiation, and accumulated within cancers. Based on these considerations, several radionuclides (tritium, copper-64, gallium-67, iodine-123, iodine 125, iodine-131 and astatine-211) were chosen and their toxicity was assessed using cell division in mammalian cultured cells as a criterion. It was apparent that the toxic effects obtained with 125 I greatly exceeded those observed in cells treated with any other radionuclides. The possible hypotheses to explain the excessive radiosensitivity of 125 I were discussed in relation to microdosimetry calculation. It was also found that the division delay induced by radionuclide decay is primarily due to damage to the cell nucleus but not to the plasma membrane. The key problem remains the development of agents which can serve as carriers for radionuclide accumulation within tumors. Although several promising approaches (Synkavit, tamoxifen, iododeoxyuridine, antibodies, liposomes) were investigated, only 125 I-labelled Synkavit would be desirable for clinical application

Resveratrol analogue 3,4,4′,5-tetramethoxystilbene inhibits growth, arrests cell cycle and induces apoptosis in ovarian SKOV‐3 and A-2780 cancer cells

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Piotrowska, Hanna; Myszkowski, Krzysztof; Ziółkowska, Alicja [Department of Toxicology, Poznan University of Medical Sciences, Poznan (Poland); Kulcenty, Katarzyna [Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan (Poland); Wierzchowski, Marcin [Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, Poznan (Poland); Kaczmarek, Mariusz [Department of Clinical Immunology, Poznan University of Medical Sciences, Poznan (Poland); Murias, Marek [Department of Toxicology, Poznan University of Medical Sciences, Poznan (Poland); Kwiatkowska-Borowczyk, Eliza [Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan (Poland); Department of Cancer Diagnostics and Immunology, Greater Poland Cancer Centre, Poznan (Poland); Jodynis-Liebert, Jadwiga, E-mail: liebert@ump.edu.pl [Department of Toxicology, Poznan University of Medical Sciences, Poznan (Poland)

2012-08-15

In the screening studies, cytotoxicity of 12 methylated resveratrol analogues on 11 human cancer cell lines was examined. The most active compound 3,4,4′5-tetramethoxystilbene (DMU-212) and two ovarian cancer cell lines A-2780 (IC{sub 50} = 0.71 μM) and SKOV-3 (IC{sub 50} = 11.51 μM) were selected for further investigation. To determine the mechanism of DMU-212 cytotoxicity, its ability to induce apoptosis was examined. DMU-212 arrested cell cycle in the G2/M or G0/G1 phase which resulted in apoptosis of both cell lines. The expression level of 84 apoptosis-related genes was investigated. In SKOV-3 cells DMU-212 caused up-regulation of pro-apoptotic Bax, Apaf-1 and p53 genes, specific to intrinsic pathway of apoptosis, and a decrease in Bcl-2 and Bcl 2110 mRNA expressions. Conversely, in A-2780 cells an increased expression of pro-apoptotic genes Fas, FasL, TNF, TNFRSF10A, TNFRSF21, TNFRSF16 specific to extracellular mechanism of apoptosis was observed. There are no data published so far regarding the receptor mediated apoptosis induced by DMU-212. The activation of caspase-3/7 was correlated with decreased TRAF-1 and BIRC-2 expression level in A-2780 cells exposed to DMU-212. DMU-212 caused a decrease in CYP1A1 and CYP1B1 mRNA levels in A-2780 by 50% and 75%, and in SKOV-3 cells by 15% and 45%, respectively. The protein expression was also reduced in both cell lines. It is noteworthy that the expression of CYP1B1 protein was entirely inhibited in A-2780 cells treated with DMU-212. It can be suggested that different CYP1B1 expression patterns in either ovarian cell line may affect their sensitivity to cytotoxic activity of DMU-212. -- Highlights: ► DMU-212 was the most cytotoxic among 12 O-methylated resveratrol analogues. ► DMU-212 arrested cell cycle at G2/M and G0/G1phase ► DMU-212 triggered mitochondria- and receptor‐mediated apoptosis. ► DMU-212 entirely inhibited CYP1B1 protein expression in A-2780 cells.

Resveratrol analogue 3,4,4′,5-tetramethoxystilbene inhibits growth, arrests cell cycle and induces apoptosis in ovarian SKOV‐3 and A-2780 cancer cells

International Nuclear Information System (INIS)

Piotrowska, Hanna; Myszkowski, Krzysztof; Ziółkowska, Alicja; Kulcenty, Katarzyna; Wierzchowski, Marcin; Kaczmarek, Mariusz; Murias, Marek; Kwiatkowska-Borowczyk, Eliza; Jodynis-Liebert, Jadwiga

2012-01-01

In the screening studies, cytotoxicity of 12 methylated resveratrol analogues on 11 human cancer cell lines was examined. The most active compound 3,4,4′5-tetramethoxystilbene (DMU-212) and two ovarian cancer cell lines A-2780 (IC 50 = 0.71 μM) and SKOV-3 (IC 50 = 11.51 μM) were selected for further investigation. To determine the mechanism of DMU-212 cytotoxicity, its ability to induce apoptosis was examined. DMU-212 arrested cell cycle in the G2/M or G0/G1 phase which resulted in apoptosis of both cell lines. The expression level of 84 apoptosis-related genes was investigated. In SKOV-3 cells DMU-212 caused up-regulation of pro-apoptotic Bax, Apaf-1 and p53 genes, specific to intrinsic pathway of apoptosis, and a decrease in Bcl-2 and Bcl 2110 mRNA expressions. Conversely, in A-2780 cells an increased expression of pro-apoptotic genes Fas, FasL, TNF, TNFRSF10A, TNFRSF21, TNFRSF16 specific to extracellular mechanism of apoptosis was observed. There are no data published so far regarding the receptor mediated apoptosis induced by DMU-212. The activation of caspase-3/7 was correlated with decreased TRAF-1 and BIRC-2 expression level in A-2780 cells exposed to DMU-212. DMU-212 caused a decrease in CYP1A1 and CYP1B1 mRNA levels in A-2780 by 50% and 75%, and in SKOV-3 cells by 15% and 45%, respectively. The protein expression was also reduced in both cell lines. It is noteworthy that the expression of CYP1B1 protein was entirely inhibited in A-2780 cells treated with DMU-212. It can be suggested that different CYP1B1 expression patterns in either ovarian cell line may affect their sensitivity to cytotoxic activity of DMU-212. -- Highlights: ► DMU-212 was the most cytotoxic among 12 O-methylated resveratrol analogues. ► DMU-212 arrested cell cycle at G2/M and G0/G1phase ► DMU-212 triggered mitochondria- and receptor‐mediated apoptosis. ► DMU-212 entirely inhibited CYP1B1 protein expression in A-2780 cells.

Cannabinoid type 1 receptor ligands WIN 55,212-2 and AM 251 alter anxiety-like behaviors of marmoset monkeys in an open-field test.

Science.gov (United States)

Cagni, Priscila; Barros, Marilia

2013-03-01

Cannabinoid type 1 receptors (CB1r) are an important modulatory site for emotional behavior. However, little is known on the effects of CB1r ligands on emotionality aspects of primates, even with their highly similar behavioral response and receptor density/distribution as humans. Thus, we analyzed the effects of the CB1r agonist WIN 55,212-2 (WIN; 1mg/kg) and the antagonist AM 251 (AM; 2mg/kg), systemically administered prior to a single brief (15 min) exposure to a novel open-field (OF) environment, on the behavior of individually tested adult black tufted-ear marmosets. Both WIN- and AM-treated subjects, compared to vehicle controls, had significantly lower rates of long (contact) calls and exploration, while higher levels of vigilance-related behaviors (scan/glance); these are indicators of anxiolysis in this setup. Changes in locomotion were not detected. However, in the vehicle and AM-groups, sojourn in the peripheral zone of the OF was significantly higher than in its central region. WIN-treated marmosets spent an equivalent amount of time in both zones. Therefore, activation or blockade CB1r function prior to a short and individual exposure to an unfamiliar environment exerted a significant and complex influence on different behavioral indicators of anxiety in these monkeys (i.e., a partially overlapping anxiolytic-like profile). AM 251, however, has no anxiolytic effect when the time spent in the center of the OF is considered. This is a major difference when compared to the WIN-treated group. Data were compared to the response profile reported in other pre-clinical (rodent) and clinical studies. Copyright © 2012 Elsevier B.V. All rights reserved.

Elucidation of a Carotenoid Biosynthesis Gene Cluster Encoding a Novel Enzyme, 2,2′-β-Hydroxylase, from Brevundimonas sp. Strain SD212 and Combinatorial Biosynthesis of New or Rare Xanthophylls

Science.gov (United States)

Nishida, Yasuhiro; Adachi, Kyoko; Kasai, Hiroaki; Shizuri, Yoshikazu; Shindo, Kazutoshi; Sawabe, Akiyoshi; Komemushi, Sadao; Miki, Wataru; Misawa, Norihiko

2005-01-01

A carotenoid biosynthesis gene cluster mediating the production of 2-hydroxyastaxanthin was isolated from the marine bacterium Brevundimonas sp. strain SD212 by using a common crtI sequence as the probe DNA. A sequence analysis revealed this cluster to contain 12 open reading frames (ORFs), including the 7 known genes, crtW, crtY, crtI, crtB, crtE, idi, and crtZ. The individual ORFs were functionally analyzed by complementation studies using Escherichia coli that accumulated various carotenoid precursors due to the presence of other bacterial crt genes. In addition to functionally identifying the known crt genes, we found that one (ORF11, named crtG) coded for a novel enzyme, carotenoid 2,2′-β-hydroxylase, which showed intriguingly partial homology with animal sterol-C5-desaturase. When this crtG gene was introduced into E. coli accumulating zeaxanthin and canthaxanthin, the resulting transformants produced their 2-hydroxylated and 2,2′-dihydroxylated products which were structurally novel or rare xanthophylls, as determined by their nuclear magnetic resonance and high-performance liquid chromatography/photodiode array detector/atmospheric pressure chemical ionization mass spectrometry spectral data. The new carotenoid produced was suggested to have a strong inhibitory effect on lipid peroxidation. PMID:16085816

Consequences of Adolescent Exposure to the Cannabinoid Receptor Agonist WIN55,212-2 on Working Memory in Female Rats

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Erin K. Kirschmann

2017-07-01

Full Text Available Marijuana is a prevalent illicit substance used by adolescents, and several studies have indicated that adolescent use can lead to long-term cognitive deficits including problems with attention and memory. However, preclinical animal studies that observe cognitive deficits after cannabinoid exposure during adolescence utilize experimenter administration of doses of cannabinoids that may exceed what an organism would choose to take, suggesting that contingency and dose are critical factors that need to be addressed in translational models of consequences of cannabinoid exposure. Indeed, we recently developed an adolescent cannabinoid self-administration paradigm in male rats, and found that prior adolescent self-administration of the cannabinoid receptor agonist WIN55,212-2 (WIN resulted in improved working memory performance in adulthood. In addition, the doses self-administered were not as high as those that are found to produce memory deficits. However, given known sex differences in both drug self-administration and learning and memory processes, it is possible that cannabinoid self-administration could have different cognitive consequences in females. Therefore, we aimed to explore the effects of self-administered vs. experimenter-administered WIN in adolescent female rats on adult cognitive function. Female rats were trained to self-administer WIN daily throughout adolescence (postnatal day 34–59. A control group self-administered vehicle solution. The acute effects of adolescent WIN self-administration on memory were determined using a short-term spatial memory test 24 h after final SA session; and the long-term effects on cognitive performance were assessed during protracted abstinence in adulthood using a delayed-match-to-sample working memory task. In a separate experiment, females were given daily intraperitoneal (IP injections of a low or high dose of WIN, corresponding to self-administered and typical experimenter

Consequences of Adolescent Exposure to the Cannabinoid Receptor Agonist WIN55,212-2 on Working Memory in Female Rats.

Science.gov (United States)

Kirschmann, Erin K; McCalley, Daniel M; Edwards, Caitlyn M; Torregrossa, Mary M

2017-01-01

Marijuana is a prevalent illicit substance used by adolescents, and several studies have indicated that adolescent use can lead to long-term cognitive deficits including problems with attention and memory. However, preclinical animal studies that observe cognitive deficits after cannabinoid exposure during adolescence utilize experimenter administration of doses of cannabinoids that may exceed what an organism would choose to take, suggesting that contingency and dose are critical factors that need to be addressed in translational models of consequences of cannabinoid exposure. Indeed, we recently developed an adolescent cannabinoid self-administration paradigm in male rats, and found that prior adolescent self-administration of the cannabinoid receptor agonist WIN55,212-2 (WIN) resulted in improved working memory performance in adulthood. In addition, the doses self-administered were not as high as those that are found to produce memory deficits. However, given known sex differences in both drug self-administration and learning and memory processes, it is possible that cannabinoid self-administration could have different cognitive consequences in females. Therefore, we aimed to explore the effects of self-administered vs. experimenter-administered WIN in adolescent female rats on adult cognitive function. Female rats were trained to self-administer WIN daily throughout adolescence (postnatal day 34-59). A control group self-administered vehicle solution. The acute effects of adolescent WIN self-administration on memory were determined using a short-term spatial memory test 24 h after final SA session; and the long-term effects on cognitive performance were assessed during protracted abstinence in adulthood using a delayed-match-to-sample working memory task. In a separate experiment, females were given daily intraperitoneal (IP) injections of a low or high dose of WIN, corresponding to self-administered and typical experimenter-administered doses, respectively, or

A Rosa canina - Urtica dioica - Harpagophytum procumbens/zeyheri Combination Significantly Reduces Gonarthritis Symptoms in a Randomized, Placebo-Controlled Double-Blind Study.

Science.gov (United States)

Moré, Margret; Gruenwald, Joerg; Pohl, Ute; Uebelhack, Ralf

2017-12-01

The special formulation MA212 (Rosaxan) is composed of rosehip ( Rosa canina L.) puree/juice concentrate, nettle ( Urtica dioica L.) leaf extract, and devil's claw ( Harpagophytum procumbens DC. ex Meisn. or Harpagophytum zeyheri Decne.) root extract and also supplies vitamin D. It is a food for special medical purposes ([EU] No 609/2013) for the dietary management of pain in patients with gonarthritis.This 12-week randomized, placebo-controlled double-blind parallel-design study aimed to investigate the efficacy and safety of MA212 versus placebo in patients with gonarthritis.A 3D-HPLC-fingerprint (3-dimensional high pressure liquid chromatography fingerprint) of MA212 demonstrated the presence of its herbal ingredients. Ninety-two randomized patients consumed 40 mL of MA212 (n = 46) or placebo (n = 44) daily. The Western Ontario and McMaster Universities Arthritis Index (WOMAC), quality-of-life scores at 0, 6, and 12 weeks, and analgesic consumption were documented. Statistically, the initial WOMAC subscores/scores did not differ between groups. During the study, their means significantly improved in both groups. The mean pre-post change of the WOMAC pain score (primary endpoint) was 29.87 in the MA212 group and 10.23 in the placebo group. The group difference demonstrated a significant superiority in favor of MA212 (p U  < 0.001; p t  < 0.001). Group comparisons of all WOMAC subscores/scores at 6 and 12 weeks reached same significances. Compared to placebo, both physical and mental quality of life significantly improved with MA212. There was a trend towards reduced analgesics consumption with MA212, compared to placebo. In the final efficacy evaluation, physicians (p Chi  < 0.001) and patients (p Chi  < 0.001) rated MA212 superior to placebo. MA212 was well tolerated.This study demonstrates excellent efficacy for MA212 in gonarthritis patients. Georg Thieme Verlag KG Stuttgart · New York.

Production of Astatine-211 at the Duke University Medical Center for its regional distribution

Energy Technology Data Exchange (ETDEWEB)

Zalutsky, Michael [Duke University Medical Center, Durham, NC (United States)

2016-01-01

Systemic targeted radiation therapy and radioimmunotherapy continue to be important tools in the treatment of certain cancers. Because of their high energy and short path length, alpha particle emitters such as ²¹¹At are more effective than either external beam x- ray or in vivo beta radiation in delivering potentially curative doses of radiation. The limited clinical trials that have been conducted to date have yielded encouraging responses in some patients, e.g., malignant brain tumors. In order to escalate the additional necessary research and development in radiochemistry, radiobiology and efficacy evaluation of alpha particle radiotherapeutics, it is universally agreed that access to an affordable, reliable supply of ²¹¹At is warranted. In conjunction with the Department of Energy's intent to enhance stable and radioactive isotope availability for research applications, it is the primary objective of this project to improve ²¹¹At production and purification capabilities at Duke so that this radionuclide can be supplied to researchers at other institutions throughout the US.The most widely used ²¹¹At production method involves the α,2n reaction on Bismuth using a cyclotron with beams ≤ 28 MeV. Yields can be enhanced with use of an internal target that allows for a higher alpha fluence plus efficient heat dissipation in the target. Both of these items are in place at Duke; however, in order to support production for multi-institutional use, irradiation campaigns in excess of 50 µAp and four hours duration will be needed. Further, post-irradiation processing equipment is lacking that will enable the distribution process. Financial support is sought for i) a shielded, ventilated processing/containment hood; ii) development of a post-irradiation target retrieval system; iii) fabrication of a ²¹¹At distillation and recovery module and iv) a performance review and, where needed, an enhancement of seven major subsystems that comprise the CS-30 Cyclotron. With these modifications in place, routine production of ≥200 mCi of At-211 should be readily achievable, given our methodological development of At-211 target preparation, internal target irradiation and dry distillation to recover the radionuclide.

77 FR 59339 - Acquisition of Commercial Items

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2012-09-27

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 212 Acquisition of Commercial Items CFR Correction 212.504 [Corrected] In Title 48 of the Code of Federal Regulations, Chapter 2 (Parts 201--299), revised as of October 1, 2011, on page 73, in section 212.504, paragraph (a) is...

32 CFR 212.6 - Procedures.

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2010-07-01

...., v. Chicago School Reform Board of Trustees, et al.) (d) A non-Federal entity covered by this part... entities shall comply with applicable fire and safety regulations; environmental laws; local, State, and...

12 CFR 212.2 - Definitions.

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2010-01-01

... person whose management functions relate exclusively to the business of retail merchandising or... Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM MANAGEMENT OFFICIAL... immediate family. “Immediate family” means spouse, mother, father, child, grandchild, sister, brother, or...

40 CFR 21.2 - Definitions.

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2010-07-01

..., wrecked or discarded equipment, rock, sand, cellar dirt and industrial, municipal, and agricultural waste...; Provided, That it does not mean preparation or undertaking of: Plans to determine feasibility; engineering..., agricultural, forest practices, mining, construction; ground or surface water pollution from well, subsurface...

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2010-01-01

... administrative functions as it may deem necessary and define the duties and determine the compensation of each... information, and industry information programs contemplated therein. (g) To maintain such books and records... accounting with respect to the receipt and disbursement of all funds entrusted to it. (h) With the approval...

19 CFR 10.212 - Definitions.

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2010-04-01

...) which has been the subject of a finding by the President or his designee, published in the Federal... Opportunity Act (19 U.S.C. 3722) and which the President has designated as a beneficiary sub-Saharan African... Mexico on December 17, 1992. Originating. “Originating” means having the country of origin determined by...

BDML Metadata: 212 [SSBD[Archive

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Full Text Available NC-SA 0.150 9c0f0803-db21-4275-b9a0-1b4dd952537e 0.105 x 0.105 x 0.5 (micrometer), 40 (second) http://ssbd.qbic.riken.jp/data.../source/Ce_KK_P002/RNAi_T17E9.2_040902_01/ http://ssbd.qbic.riken.jp/data.../bdml/Ce_KK_P002/RNAi_T17E9.2_040902_01.bdml0.15.xml http://ssbd.qbic.riken.jp/data/pdpml/Ce_KK_P00...2.pdpml0.05.xml http://ssbd.qbic.riken.jp/search/9c0f0803-db21-4275-b9a0-1b4dd952537e/ http://ssbd.qbic.riken.jp/omero/webclient/?show=dataset-156 ...

40 CFR 94.212 - Labeling.

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2010-07-01

... contain the following information lettered in the English language in block letters and numerals, which... auxiliary, variable-speed propulsion engines used with fixed-pitch propellers, recreational, etc.) (c) The... VIOLATION OF FEDERAL LAW SUBJECT TO CIVIL PENALTIES.” The Administrator may approve alternate language. [64...

25 CFR 212.3 - Definitions.

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2010-04-01

... to which is held in trust by the United States or is subject to restriction against alienation... by the United States, or is subject to the restriction against alienation imposed by the United... trust by the United States, or is subject to restriction against alienation imposed by the United States...

42 CFR 2.12 - Applicability.

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2010-10-01

..., employee assistance programs, programs within general hospitals, school-based programs, and private... making a referral for the treatment. (b) Federal assistance. An alcohol abuse or drug abuse program is... community as a provider of such services. (2) Federal assistance to program required. If a patient's alcohol...

MicroRNAs and drug addiction

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Paul J Kenny

2013-05-01

Full Text Available Drug addiction is considered a disorder of neuroplasticity in brain reward and cognition systems resulting from aberrant activation of gene expression programs in response to prolonged drug consumption. Noncoding RNAs are key regulators of almost all aspects of cellular physiology. MicroRNAs (miRNAs are small (~21–23 nucleotides noncoding RNA transcripts that regulate gene expression at the post-transcriptional level. Recently, microRNAs were shown to play key roles in the drug-induced remodeling of brain reward systems that likely drives the emergence of addiction. Here, we review evidence suggesting that one particular miRNA, miR-212, plays a particularly prominent role in vulnerability to cocaine addiction. We review evidence showing that miR-212 expression is increased in the dorsal striatum of rats that show compulsive-like cocaine-taking behaviors. Increases in miR-212 expression appear to protect against cocaine addiction, as virus-mediated striatal miR-212 over-expression decreases cocaine consumption in rats. Conversely, disruption of striatal miR-212 signaling using an antisense oligonucleotide increases cocaine intake. We also review data that identify two mechanisms by which miR-212 may regulate cocaine intake. First, miR-212 has been shown to amplify striatal CREB signaling through a mechanism involving activation of Raf1 kinase. Second, miR-212 was also shown to regulate cocaine intake by repressing striatal expression of methyl CpG binding protein 2 (MeCP2, consequently decreasing protein levels of brain-derived neurotrophic factor (BDNF. The concerted actions of miR-212 on striatal CREB and MeCP2/BDNF activity greatly attenuate the motivational effects of cocaine. These findings highlight the unique role for miRNAs in simultaneously controlling multiple signaling cascades implicated in addiction.

77 FR 55796 - Sand Lick Fork Watershed Restoration Project; Daniel Boone National Forest, KY

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2012-09-11

..., conversion of 0.6 miles of Powell County Road 212 to Forest Service maintenance, conversion of 3.1 miles of..., 212A, 2045, 2120 and the section of county road to be transferred to Forest Service maintenance... miles of Powell County Road 212 to Forest Service maintenance, conversion of 3.1 miles of system roads...

Enhanced self-administration of the CB1 receptor agonist WIN55,212-2 in olfactory bulbectomized rats: evaluation of possible serotonergic and dopaminergic underlying mechanisms

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Petra eAmchova

2014-03-01

Full Text Available Depression has been associated with drug consumption, including heavy or problematic cannabis use. According to an animal model of depression and substance use disorder comorbidity, we combined the olfactory bulbectomy model of depression with intravenous drug self-administration procedure to verify whether depressive-like rats displayed higher voluntary intake of the CB1 receptor agonist WIN55,212-2 (WIN, 12.5 µg/kg/infusion. To this aim, olfactory-bulbectomized (OBX and sham-operated (SHAM Lister Hooded rats were allowed to self-administer WIN by lever-pressing under a continuous (FR-1 schedule of reinforcement in 2h daily sessions. Data showed that both OBX and SHAM rats developed stable WIN intake; yet, responses in OBX were constantly higher than in SHAM rats soon after the first week of training. In addition, OBX rats took significantly longer to extinguish the drug-seeking behaviour after vehicle substitution. Acute pre-treatment with serotonin 5HT1B receptor agonist, CGS-12066B (2.5-10 mg/kg, did not significantly modify WIN intake in OBX and SHAM Lister Hooded rats. Furthermore, acute pre-treatment with CGS-12066B (10 and 15 mg/kg did not alter responses in parallel groups of OBX and SHAM Sprague Dawley rats self-administering methamphetamine under higher (FR-2 reinforcement schedule with nose-poking as operandum. Finally, dopamine levels in the nucleus accumbens of OBX rats did not increase in response to a WIN challenge, as in SHAM rats, indicating a dopaminergic dysfunction in bulbectomized rats. Altogether, our findings suggest that a depressive state may alter cannabinoid CB1 receptor agonist-induced brain reward function and that a dopaminergic rather than a 5-HT1B mechanism is likely to underlie enhanced WIN self-administration in OBX rats.

Coordination chemistry of the sup 212 Pb/ sup 212 Bi nuclear transformation: Alpha-emitting radiopharmaceuticals

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Parks, N.J.; Harris, W.R.; Keen, C.L.; Cooper, S.R.

1992-07-01

Subdivisions of this project are: (a) the synthesis of prototypical thiolate and dithiocarbamate based hexacoordinate complexes, (b) radiochemical engineering for generation of no-carrier-added lead and bismuth radioelements, (c) the first isolation of bismuth-binding proteins from in vivo studies with cyclotron produced {sup 205/206}Bi tracer, and (d) initial development of transport mechanisms for the intracellular radiobiological study of alpha emitting bismuth, and (e) the initiation of chemical equilibrium studies and biochemical pathways with cyclotron-produced, no-carrier-added, {sup 203}Pb (T{sub 1/2} = 51 hr).

Integrated Modeling and Analysis of Physical Oceanographic and Acoustic Processes

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2015-09-30

Institute of Technology Cambridge, MA 02139 phone: (617) 324-5172 fax: (617) 324-3451 email: pierrel@mit.edu Nicholas C. Makris Massachusetts...the phase function Γ so that α is tan -1(py / px). The canonical coordinates are ray position components (x and y) and phase gradient components...SSSS ds dp SSSS ds dy SSSS ds dx 1 2/12 2 2/12 2 2/12 2 2/12 2 tan cossin sincos α θ θ θ αα θ θ αα θ αθ αθ αθαθ αθαθ Here, s is the arc length along

76 FR 33776 - Western Hemisphere Travel Initiative: Designation of an Approved Native American Tribal Card...

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2011-06-09

... the Intelligence Reform and Terrorism Prevention Act of 2004 (IRTPA), Public Law 108-458, as amended....'' This definition applies to 8 CFR 212.1 and 235.1. Upon designation by the Secretary of Homeland... States. See 8 CFR 235.1(b)(7). \\2\\ See 8 CFR 212.0. This definition applies to 8 CFR 212.1 and 235.1. The...

Nuclear and chemical data for life sciences

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Moumita Maiti; Indian Institute of Technology Roorkee, Roorkee, Uttarakhand

2013-01-01

Use of reactor produced radionuclides is popular in life sciences. However, cyclotron production of proton rich radionuclides are being more focused in recent times. These radionuclides have already gained attention in various fields, including life sciences, provided they are obtained in pure form. This article is a representative brief of our contributions in generating nuclear data for the production of proton rich radionuclides of terbium, astatine, technetium, ruthenium, cadmium, niobium, zirconium, rhenium, etc., which may have application in clinical, biological, agriculture studies or in basic research. The chemical data required to separate the product isotopes from the corresponding target matrix have been presented along with a few propositions of radiopharmaceuticals. It also emphasizes on the development of simple empirical technique, based on the nuclear reaction model analysis, to generate reliable nuclear data for the estimation of yield and angular distribution of emitted neutrons and light charged particles from light as well as heavy ion induced reactions on thick stopping targets. These data bear utmost important in radiation dosimetry. (author)

Study of Neutron-Deficient $^{202-205}$Fr Isotopes with Collinear Resonance Ionization Spectroscopy

CERN Document Server

De Schepper, Stijn; Cocolios, Thomas; Budincevic, Ivan

The scope of this master’s thesis is the study of neutron-deficient $^{202−205}$Fr isotopes. These isotopes are inside the neutron-deficient lead region, a region that has shown evidence of shape coexistence. For this thesis, this discussion is limited to the phenomenon where a low lying excited state has a different shape than the ground state. Shape coexistence is caused by intruder states. These are single-particle Shell Model states that are perturbed in energy due to the interaction with a deformed core. In the neutron-deficient lead region the main proton intruder orbit is the 3s$_{1/2}$orbit. When going towards more neutron-deficient isotopes, deformation increases. The $\\pi3s_{1/2}$orbit will rise in energy and will eventually become the ground state in odd- A bismuth (Z=83) isotopes. It is also observed in odd-A astatine (Z=85) isotopes, already in less neutron-deficient nuclei. The same phenomenon is expected to be present francium (Z=87) isotopes already at $^{199}$Fr. Although it is currently ...

Development of Reagents for Application of At-211 to Targeted Radionuclide Therapy of Cancer

International Nuclear Information System (INIS)

Wilbur, D. Scott

2011-01-01

This grant covered only a period of 4 months as the major portion of the award was returned to DOE due to an award of funding from NIH that covered the same research objectives. A letter regarding the termination of the research is attached as the last page of the Final Report. The research conducted was limited due to the short period of this grant, but the results obtained in that period are outlined in the Final Report. The studies addressed in the research effort were directed at a problem that is of critical importance to the in vivo application of the alpha-particle emitting radionuclide At-211. That problem, low in vivo stability of many astatinated molecules, severely limits the use of At-211 in therapeutic applications. The advances sought in the studies were expected to expand the types of biomolecules that can be used as carriers of At-211, and provide improved in vivo targeting of the radiation dose compared with the dose delivered to normal tissue.

Sci-Thur AM: YIS – 01: New technologies for astatine-211 targeted alpha therapy research

Energy Technology Data Exchange (ETDEWEB)

Crawford, Jason; Yang, Hua; Schaffer, Paul; Ruth, Thomas [University of Victoria, Victoria, BC (Canada); TRIUMF, Vancouver, BC (Canada)

2016-08-15

Purpose: The short-range, densely ionizing α-particles emitted by {sup 211}At (t{sub 1/2}=7.2h) are well suited for the treatment of diffuse microscopic disease, using cancer targeting biomolecules. {sup 211}At availability is limited by the rarity of α-cyclotrons required for standard production. Image-based dosimetry is also limited for {sup 211}At, which emits low intensity X-rays. Our goal was to leverage state-of-the-art infrastructure at TRIUMF to produce and evaluate two related isotopes, {sup 211}Rn (t{sub 1/2}=14.6h, 73% decay to {sup 211}At) as a generator for {sup 211}At, and {sup 209}At (t{sub 1/2}=5.4h, X-ray/gamma-ray emitter) as a novel 211At surrogate for preclinical imaging studies. Methods: Produced by spallation of uranium with 480 MeV protons, mass separated ion beams of short-lived francium isotopes were implanted into NaCl targets where {sup 211}Rn or {sup 209}At were produced by radioactive decay, in situ. {sup 211}Rn was transferred to dodecane from which {sup 211}At was efficiently extracted and evaluated for clinical applicability. High energy SPECT/CT was evaluated for measuring {sup 209}At activity distributions in mice and phantoms. Results: Our small scale {sup 211}Rn/{sup 211}At generator system provided high purity {sup 211}At samples. The methods are immediately scalable to the level of radioactivity required for in vivo experiments with {sup 211}At. {sup 209}At-based high energy SPECT imaging was determined suitable for pursuing image-based dosimetry in mouse tumour models. In the future, we will utilize quantitative {sup 209}At-SPECT for image-based dose calculations. Conclusion: These early studies provided a foundation for future endeavours with {sup 211}At-based α-therapy. Canada is now significantly closer to clinical targeted α-therapy of cancer.

Reaction of aromatic diazonium salts with carrier-free radioiodine and astatine, evidence for complex formation

International Nuclear Information System (INIS)

Meyer, G.J.; Roessler, K.; Stoecklin, G.

1979-01-01

Systematic studies of the astatodiazoniation reaction and a comparison with iododediazoniation under comparable conditions are reported. The yields for all astatohalobenzenes and -toluenes were nearly constant and unaffected by the nature of the diazonium compound, its isomeric form, and the number of isomers used at the same time. Only astatofluorobenzenes were obtained at higher yields. An electron-transfer mechanism is proposed for dediazoniation at these low halide concentration levels. At sufficient thermal excitation levels the electron transfer leads to the dissociation of nitrogen, while the phenyl and halogen radicals recombine. The isomer distribution found for some of the derivatives from dediazoniation may also be due to steric effects

Preparation and evaluation of an astatine-211-labeled sigma receptor ligand for alpha radionuclide therapy

International Nuclear Information System (INIS)

Ogawa, Kazuma; Mizuno, Yoshiaki; Washiyama, Kohshin; Shiba, Kazuhiro; Takahashi, Naruto; Kozaka, Takashi; Watanabe, Shigeki; Shinohara, Atsushi; Odani, Akira

2015-01-01

Introduction: Sigma receptors are overexpressed in a variety of human tumors, making them potential targets for radionuclide receptor therapy. We have previously synthesized and evaluated 131 I-labeled (+)-2-[4-(4-iodophenyl)piperidino]cyclohexanol [(+)-[ 131 I]pIV], which has a high affinity for sigma receptors. Therefore, (+)-[ 131 I]pIV significantly inhibited tumor cell proliferation in tumor-bearing mice. In the present study, we report the synthesis and the in vitro and in vivo characterization of (+)-[ 211 At]pAtV, an 211 At-labeled sigma receptor ligand, that has potential use in alpha-radionuclide receptor therapy. Methods: The radiolabeled sigma receptor ligand (+)-[ 211 At]pAtV was prepared using a standard halogenation reaction generating a 91% radiochemical yield with 98% purity after HPLC purification. The partition coefficient of (+)-[ 211 At]pAtV was measured. Cellular uptake experiments and in vivo biodistribution experiments were performed using a mixed solution of (+)-[ 211 At]pAtV and (+)-[ 125 I]pIV; the human prostate cancer cell line DU-145, which expresses high levels of the sigma receptors, and DU-145 tumor-bearing mice. Results: The lipophilicity of (+)-[ 211 At]pAtV was similar to that of (+)-[ 125 I]pIV. DU-145 cellular uptake and the biodistribution patterns in DU-145 tumor-bearing mice at 1 h post-injection were also similar between (+)-[ 211 At]pAtV and (+)-[ 125 I]pIV. Namely, (+)-[ 211 At]pAtV demonstrated high uptake and retention in tumor via binding to sigma receptors. Conclusion: These results indicate that (+)-[ 211 At]pAtV could function as an new agent for alpha-radionuclide receptor therapy.

Preparation and evaluation of an astatine-211-labeled sigma receptor ligand for alpha radionuclide therapy.

Science.gov (United States)

Ogawa, Kazuma; Mizuno, Yoshiaki; Washiyama, Kohshin; Shiba, Kazuhiro; Takahashi, Naruto; Kozaka, Takashi; Watanabe, Shigeki; Shinohara, Atsushi; Odani, Akira

2015-11-01

Sigma receptors are overexpressed in a variety of human tumors, making them potential targets for radionuclide receptor therapy. We have previously synthesized and evaluated (131)I-labeled (+)-2-[4-(4-iodophenyl)piperidino]cyclohexanol [(+)-[(131)I]pIV], which has a high affinity for sigma receptors. Therefore, (+)-[(131)I]pIV significantly inhibited tumor cell proliferation in tumor-bearing mice. In the present study, we report the synthesis and the in vitro and in vivo characterization of (+)-[(211)At]pAtV, an (211)At-labeled sigma receptor ligand, that has potential use in alpha-radionuclide receptor therapy. The radiolabeled sigma receptor ligand (+)-[(211)At]pAtV was prepared using a standard halogenation reaction generating a 91% radiochemical yield with 98% purity after HPLC purification. The partition coefficient of (+)-[(211)At]pAtV was measured. Cellular uptake experiments and in vivo biodistribution experiments were performed using a mixed solution of (+)-[(211)At]pAtV and (+)-[(125)I]pIV; the human prostate cancer cell line DU-145, which expresses high levels of the sigma receptors, and DU-145 tumor-bearing mice. The lipophilicity of (+)-[(211)At]pAtV was similar to that of (+)-[(125)I]pIV. DU-145 cellular uptake and the biodistribution patterns in DU-145 tumor-bearing mice at 1h post-injection were also similar between (+)-[(211)At]pAtV and (+)-[(125)I]pIV. Namely, (+)-[(211)At]pAtV demonstrated high uptake and retention in tumor via binding to sigma receptors. These results indicate that (+)-[(211)At]pAtV could function as an new agent for alpha-radionuclide receptor therapy. Copyright © 2015 Elsevier Inc. All rights reserved.

Effects of cannabinoid and vanilloid receptor agonists and their interaction on learning and memory in rats.

Science.gov (United States)

Shiri, Mariam; Komaki, Alireza; Oryan, Shahrbanoo; Taheri, Masoumeh; Komaki, Hamidreza; Etaee, Farshid

2017-04-01

Despite previous findings on the effects of cannabinoid and vanilloid systems on learning and memory, the effects of the combined stimulation of these 2 systems on learning and memory have not been studied. Therefore, in this study, we tested the interactive effects of cannabinoid and vanilloid systems on learning and memory in rats by using passive avoidance learning (PAL) tests. Forty male Wistar rats were divided into the following 4 groups: (1) control (DMSO+saline), (2) WIN55,212-2, (3) capsaicin, and (4) WIN55,212-2 + capsaicin. On test day, capsaicin, a vanilloid receptor type 1 (TRPV1) agonist, or WIN55,212-2, a cannabinoid receptor (CB 1 /CB 2 ) agonist, or both substances were injected intraperitoneally. Compared to the control group, the group treated with capsaicin (TRPV1 agonist) had better scores in the PAL acquisition and retention test, whereas treatment with WIN55,212-2 (CB 1 /CB 2 agonist) decreased the test scores. Capsaicin partly reduced the effects of WIN55,212-2 on PAL and memory. We conclude that the acute administration of a TRPV1 agonist improves the rats' cognitive performance in PAL tasks and that a vanilloid-related mechanism may underlie the agonistic effect of WIN55,212-2 on learning and memory.

41 CFR 105-60.303 - Rules for public inspection and copying.

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2010-07-01

..., New York, the Commonwealth of Puerto Rico, and the Virgin Islands) General Services Administration (2AR) 26 Federal Plaza, New York, NY 10278 Telephone: 212-264-1234 FAX: 212-264-2760 Mid-Atlantic...

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Full Text Available 184486 HSA_5901938 Cluster Sequences - 212 NP_008952.1 fasting induced gene ; no an...luster sequences Cluster Sequences Link to related sequences - Sequence length 212 Representative annotation NP_008952.1 fasting

PODAAC-ASOP2-12C01

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National Aeronautics and Space Administration — This dataset contains operational near-real-time Level 2 coastal ocean surface wind vector retrievals from the Advanced Scatterometer (ASCAT) on MetOp-A at 12.5 km...

PODAAC-ASOP2-12X01

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National Aeronautics and Space Administration — This dataset contains operational near-real-time Level 2 ocean surface wind vector retrievals from the Advanced Scatterometer (ASCAT) on MetOp-A at 12.5 km sampling...

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2010-04-01

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Community-based dengue vector control : experiences in behavior change in Metropolitan Manila, Philippines (open access). Dengue is the most important mosquito-borne disease in the Philippines, especially in Metropolitan Manila where communities are socially and economically diverse, and city governments struggle ...

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2010-01-01

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2010-10-01

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... Science and Technology, Social Policy, Economics, Environment, Evaluation, Food ... for competing demands on natural resources of both water and energy sectors, and is a ... of using technologies for management of health information in hospital. ... In this paper, we present a structural learning model for joint sentiment ...

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2010-04-01

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Home Sewage Disposal. Special Circular 212.

Science.gov (United States)

Wooding, N. Henry

This circular provides current information for homeowners who must repair or replace existing on-lot sewage disposal systems. Site requirements, characteristics and preparation are outlined for a variety of alternatives such as elevated sand mounds, sand-lined beds and trenches, and oversized absorption area. Diagrams indicating construction…

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... capacity to conduct use focused evaluations using a mentoring approach - LIRNEasia's CPRsouth Program (restricted access). Utilization-focused Evaluation (UFE) was used in this case as a "self-evaluation" for the organization itself, helping to provide feedback on needs and capacities of the organization''s operations.

211 At-labeled agents for alpha-immunotherapy: On the in vivo stability of astatine-agent bonds

OpenAIRE

Ayed , Tahra ,; Pilmé , Julien; Tézé , David; Bassal , Fadel ,; Barbet , Jacques ,; Chérel , Michel; Champion , Julie ,; Maurice , Rémi; Montavon , Gilles ,; Galland , Nicolas ,

2016-01-01

International audience; The application of 211 At to targeted cancer therapy is currently hindered by the rapid deastatination that occurs in vivo. As the deastatination mechanism is unknown, we tackled this issue from the viewpoint of the intrinsic properties of At-involving chemical bonds. An apparent correlation has been evidenced between in vivo stability of 211 At-labeled compounds and the AtÀR (R ¼ C, B) bond enthalpies obtained from relativistic quantum mechanical calculations. Further...

Evaluation of Novel Wet Chemistry Separation and Purification Methods to Facilitate Automation of Astatine-211 Isolation

International Nuclear Information System (INIS)

Wilbur, Daniel Scott

2016-01-01

This research is a collaborative effort between the research groups of the PIs, Dr. D. Scott Wilbur in the Department of Radiation Oncology at the University of Washington (UW) and Matthew O'Hara at the Pacific Northwest National Laboratory (PNNL). In this report only those studies conducted at UW and the budget information from UW will be reported. A separate progress and financial report will be provided by PNNL. This final report outlines the experiments (Tasks) conducted and results obtained at UW from July 1, 2013 thru June 30, 2016 (2-year project with 1 year no-cost extension). The report divides the information on the experiments and results obtained into the 5 specific objectives of the research efforts and the Tasks within those objectives. This format is used so that it is easy to see what has been accomplished in each area. A brief summary of the major findings from the studies is provided below. Summary of Major Findings from Research/Training Activities at UW: Anion and cation exchange columns did not provide adequate 211 At capture and/or extraction results under conditions studied to warrant further evaluation; PEG-Merrifield resins containing mPEG350, mPEG750, mPEG2000 and mPEG5000 were synthesized and evaluated; All of the mPEG resins with different sized mPEG moieties conjugated gave similar 211 At capture (>95%) from 8M HCl solutions and release with conc. NH 4 OH (~50-80%), but very low quantities were released when NaOH was used as an eluent; Capture and release of 211 At when loading [ 211 At]astatate appeared to be similar to that of [ 211 At]astatide on PEG columns, but further studies need to be conducted to confirm that; Capture of 211 At on PEG columns was lower (e.g. 80-90%) from solutions of 8M HNO 3 , but higher capture rates (e.g. 99%) can be obtained when 10M HNO 3 is mixed with an equal quantity of 8M HCl; Addition of reductants to the 211 At solutions did not appear to change the percent capture, but may have an effect on the % extracted; There was some indication that the PEG-Merrifield resins could be saturated (perhaps with Bi) resulting in lower capture percentages, but more studies need to be done to confirm that; A target dissolution chamber, designed and built at PNNL, works well with syringe pumps so it can be used in an automated system; Preliminary semi-automated 211 At isolation studies have been conducted with full-scale target dissolution and 211 At isolation using a PEG column on the Hamilton automated system gave low overall recoveries, but HNO 3 was used (rather than HCl) for loading the 211 At and flow rates were not optimized; Results obtained using PEG columns are high enough to warrant further development on a fully automated system; Results obtained also indicate that additional studies are warranted to evaluate other types of columns for 211 At separation from bismuth, which allow use of HNO 3 /HCl mixtures for loading and NaOH for eluting 211 At. Such a column could greatly simplify the overall isolation process and make it easier to automate.

Preparation and biological evaluation of an astatine-211 labeled biotin conjugate: Biotinyl-3-[211At]astatoanilide

International Nuclear Information System (INIS)

Foulon, Catherine F.; Schoultz, Bent W.; Zalutsky, Michael R.

1997-01-01

Biotinyl-3-[ 211 At]astatoanilide ([ 211 At]AtBA) was prepared in more than 80% yield by destannylation. In vitro, [ 211 At]AtBA exhibited a high affinity for streptavidin, and was stable after incubation in human serum, cerebrospinal fluid and distilled water, whereas it was rapidly degraded in mouse serum. HPLC analysis showed that the main degradation pathway in mouse serum was the cleavage of [ 211 At]astatoaniline. In mice, [ 211 At]AtBA and its 125 I-labeled analogue cleared rapidly from most tissues; however, there was some evidence for dehalogenation of both tracers

Proton conduction within the reaction centers of Rhodobacter capsulatus: the electrostatic role of the protein.

OpenAIRE

Maróti, P; Hanson, D K; Baciou, L; Schiffer, M; Sebban, P

1994-01-01

Light-induced charge separation in the photosynthetic reaction center results in delivery of two electrons and two protons to the terminal quinone acceptor QB. In this paper, we have used flash-induced absorbance spectroscopy to study three strains that share identical amino acid sequences in the QB binding site, all of which lack the protonatable amino acids Glu-L212 and Asp-L213. These strains are the photosynthetically incompetent site-specific mutant Glu-L212/Asp-L213-->Ala-L212/Ala-L213 ...

Novelty-induced emotional arousal modulates cannabinoid effects on recognition memory and adrenocortical activity.

Science.gov (United States)

Campolongo, Patrizia; Morena, Maria; Scaccianoce, Sergio; Trezza, Viviana; Chiarotti, Flavia; Schelling, Gustav; Cuomo, Vincenzo; Roozendaal, Benno

2013-06-01

Although it is well established that cannabinoid drugs can influence cognitive performance, the findings-describing both enhancing and impairing effects-have been ambiguous. Here, we investigated the effects of posttraining systemic administration of the synthetic cannabinoid agonist WIN55,212-2 (0.1, 0.3, or 1.0 mg/kg) on short- and long-term retention of object recognition memory under two conditions that differed in their training-associated arousal level. In male Sprague-Dawley rats that were not previously habituated to the experimental context, WIN55,212-2 administered immediately after a 3-min training trial, biphasically impaired retention performance at a 1-h interval. In contrast, WIN55,212-2 enhanced 1-h retention of rats that had received extensive prior habituation to the experimental context. Interestingly, immediate posttraining administration of WIN55,212-2 to non-habituated rats, in doses that impaired 1-h retention, enhanced object recognition performance at a 24-h interval. Posttraining WIN55,212-2 administration to habituated rats did not significantly affect 24-h retention. In light of intimate interactions between cannabinoids and the hypothalamic-pituitary-adrenal axis, we further investigated whether cannabinoid administration might differently influence training-induced glucocorticoid activity in rats in these two habituation conditions. WIN55,212-2 administered after object recognition training elevated plasma corticosterone levels in non-habituated rats whereas it decreased corticosterone levels in habituated rats. Most importantly, following pretreatment with the corticosterone-synthesis inhibitor metyrapone, WIN55,212-2 effects on 1- and 24-h retention of non-habituated rats became similar to those seen in the low-aroused habituated animals, indicating that cannabinoid-induced regulation of adrenocortical activity contributes to the environmentally sensitive effects of systemically administered cannabinoids on short- and long

Relative biological effectiveness of alpha-particle emitters in vivo at low doses

International Nuclear Information System (INIS)

Howell, R.W.; Azure, M.T.; Narra, V.R.; Rao, D.V.

1994-01-01

The therapeutic potential of radionuclides that emit α particles, as well as their associated health hazards, have attracted considerable attention. The 224 Ra daughters 212 Pb and 212 Bi, by virtue of their radiation properties which involve emission of α and β particles in their decay to stable 208 Pb, have been proposed as candidates for radioimmunotherapy. Using mouse testes as the experimental model and testicular spermhead survival as the biological end point, the present work examines the radiotoxicity of 212 Pb and its daughters. When 212 Pb, in equilibrium with its daughters 212 Bi, 212 Po and 208 Tl, was administered directly into the testis, the dose required to achieve 37% survival (D 37 ) was 0.143 ± 0.014 Gy and the corresponding RBE of the mixed radiation field was 4.7 when compared to the D 37 for acute external 120 kVp X rays. This datum, in conjunction with our earlier results for 210 Po, was used to obtain an RBE-LET relationship for α particles emitted by tissue-incorporated radionuclides: RBE α = 4.8 - 6.1 x 10 -2 LET + 1.0 x 10 -3 LET 2 . Similarly, the dependence of RBE on α-particle energy E α was given by RBE α = 22 E α -0.73 . These relationships, based on in vivo experimental data, may be valuable in predicting biological effects of α-particle emitters. 46 refs., 6 figs

Preliminary radiological safety assessment for decommissioning of thoria dissolver of the 233U pilot plant, Trombay

International Nuclear Information System (INIS)

Priya, S.; Srinivasan, P.; Gopalakrishnan, R. K.

2012-01-01

The thoria dissolver, used for separation of 233 U from reactor-irradiated thorium metal and thorium oxide rods, is no longer operational. It was decided to carry out assessment of the radiological status of the dissolver cell for planning of the future decommissioning/dismantling operations. The dissolver interiors are expected to be contaminated with the dissolution remains of irradiated thorium oxide rods in addition to some of the partially dissolved thoria pellets. Hence, 220 Rn, a daughter product of 228 Th is of major radiological concern. Airborne activity of thoron daughters 212 Pb (Th-B) and 212 Bi (Th-C) was estimated by air sampling followed by high-resolution gamma spectrometry of filter papers. By measuring the full-energy peaks counts in the energy windows of 212 Pb, 212 Bi and 208 Tl, concentrations of thoron progeny in the sampled air were estimated by applying the respective intrinsic peak efficiency factors and suitable correction factors for the equilibration effects of 212 Pb and 212 Bi in the filter paper during the delay between sampling and counting. Then the thoron working level (TWL) was evaluated using the International Commission on Radiological Protection (ICRP) methodology. Finally, the potential effective dose to the workers, due to inhalation of thoron and its progeny during dismantling operations was assessed by using dose conversion factors recommended by ICRP. Analysis of filter papers showed a maximum airborne thoron progeny concentration of 30 TWLs inside the dissolver. (authors)

211At-labelling of polymer particles for radiotherapy: synthesis, purification and stability

International Nuclear Information System (INIS)

Larsen, R.H.; Hassfjell, S.P.; Hoff, P.; Alstad, J.; Bjoergum, J.

1993-01-01

Cyclotron-produced 211 At was distilled from a Bi metal target and coupled to N-succinimidyl-3-(trimethylstannyl)benzoate. The resulting N-succinimidyl-3-( 211 At)astatobenzoate was thereafter coupled to aminated monosized polymer particles with a diameter of 1.8 μm. The total time elapsed from the end of the cyclotron irradiation until the final product was prepared was about 2.5 hours. From 23 to 51% of the target activity at the end of bombardment was measured in the final conjugate. Solid-liquid extraction purification of the astatinated intermediate, using Sep-pak columns (Waters), gave more reproducible yields in the final conjugation step. The 211 At-labelled particles were incubated with fetal calf serum, human serum and human full blood at room temperature. The 211 At activity on the particles was measured before and after three times washing at 4, 24 and 48 hours. The stability was not significantly different from 100% for all media and for all time points. This indicates that 211 At-labelled particles can be stable under in vivo conditions, and may thereby be a promising agent for intracavitary radiotherapy on free-floating cancer cells or surface fixed cells. (Author)

Neutron metrology in the HFR

International Nuclear Information System (INIS)

Kraakman, R.; Voorbraak, W.P.

1993-04-01

Additional to the in-core EXOTIC experiments, six irradiations of ceramic material, R212-001 to R212-006, have been performed in the PSF of the HFR. This note presents the neutron metrology results for these irradiations. (orig.)

76 FR 16231 - Clarification of Countries and Geographic Areas Eligible for Participation in the Guam...

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2011-03-23

.... Accordingly, the Office of Management and Budget has not reviewed this regulation under that Executive Order... amended as set forth below: PART 212--DOCUMENTARY REQUIREMENTS: NONIMMIGRANT; WAIVERS; ADMISSION OF... read as follows: Sec. 212.1 Documentary Requirements for Nonimmigrants. * * * * * (q) * * * (2...

Fracionamento a seco da farinha de aveia e modificação química da fração rica em amido Dry fractionation of oatmeal and chemical modification of starch rich fraction

Directory of Open Access Journals (Sweden)

Ana Paula Daniel

2006-12-01

Full Text Available O estudo teve por objetivo obter frações de farinha de aveia enriquecidas em amido e em fibras pelo fracionamento a seco e modificar quimicamente (fosfatação o amido da fração rica neste constituinte, avaliando suas propriedades funcionais. O fracionamento foi efetuado utilizando-se as granulometrias > 300, 212-300, 150-212, e 300 µm e 212-300 µm foram semelhantes, com aumento de aproximadamente 1,5 e 2,7 vezes nos teores de proteína e fibra, e redução de 0,5-0,6 vezes no teor de amido, quando comparadas à farinha integral. As frações 212-150 µm e The objective of this study was to obtain starch and fiber enriched oatmeal fractions through sieving, chemically modifying (phosphorylation the starch enriched fraction, and evaluating its functional properties. Fractionation was performed using > 300, 212-300, 150-212, and 300 µm and 212-300 µm were similar and had increased protein and fiber content (1.5 and 2.7 fold, but reduced starch content (0.5-0.6 fold when compared to whole oatmeal. Fractions 212-150 µm and < 150 µm were similar and had the highest starch content (around 70%. The starch-rich fraction < 150 µm that had the highest yield during sieving (35.5% was phosphorylated with sodium tripolyphosphate at 150-155 °C for 20 and 40 min, yielding 0.39 and 0.32% phosphorus bound, respectively. Cold water binding capacity increased (1.9-3.3 fold, while syneresis at 5 °C or after freezing/thawing was significantly reduced (6-20 and 5-6 fold, respectively in phosphorylated starch fraction when compared to the native starch fraction. Phosphorylation reduced the increase of pasta opacity during storage at 5 °C, which indicates a lower retrogradation tendency. Thus, oatmeal sieving yielded fractions either enriched in fiber and protein or enriched in starch. Moreover, phosphorylation of the starch-rich fraction improved their functional properties, which may increase the potential applicability and economic value of this

Metal screen retention for thoron daughter free atoms and atoms attached to condensation nuclei

International Nuclear Information System (INIS)

Cash, W.; Webb, J.; Fitts, D.; Skrable, K.W.; Chabot, G.E.

1978-01-01

Metal support screens available in a 47 mm commercial filter holder (model F3052-4, available from Scientific Products, Bedford, MA) assembly were tested for retention of thoron daughter atoms and atoms attached to condensation nuclei as a function of the flow rate of the carrier air stream. Sources of Pb-212 were generated on the surface of a metal disk by exposing the disk to thoron emanation generated from a special preparation of Th-228. This source of Pb-212, in transient equilibrium with its daughters, was placed in a flow through chamber connected in series to two of the metal screens backed by a glass fiber filter. Most of the recoil product radioactivity emitted from the Pb-212 source and collected on the screens was due to single atoms of Tl-208, which is born by alpha decay of Bi-212 with a recoil energy of 116 keV. Some free atoms of Bi-212 were also observed. Alpha autoradiographs of Filter samples placed on the downstream side of the two metal screens gave proof of the existence of Pb-212 aggregates through their alpha star images. These aggregate recoil particles were found to have a much higher penetration through the screens than free atoms of Tl-208 and Bi-212. Penetration of Tl-208 atoms and ions decreased exponentially as the inverse of the carrier air flow rate. Penetration varied from 0.047 at 0.088 cfm to 0.661 at 2.47 cfm. Atoms of Pb-212 attached to condensation nuclei were obtained by passing thoron into a reaction chamber containing naturally occurring condensation nuclei from the laboratory. The retention for these attached species varied both as a function of the flow rate and the age of the aerosol. The maximum retention varied from 0.525% at 6.38 cfm to 3.5% at 0.636 cfm for respective delay times of 120 and 30 minutes post the introduction of the thoron into the reaction chamber. A system consisting of a single screen backed by a glass fiber filter may be used to obtain the numbers of radon or thoron daughter free atoms and attached

Lymphoma Research Foundation

Science.gov (United States)

... Follow LRF Watch LRF Contact Us National Headquarters Wall Street Plaza 88 Pine Street, Suite 2400 | New York, NY 10005 212-349-2910 | 212-349-2886 Fax LRF@lymphoma.org LRF Helpline 800-500-9976 Helpline@lymphoma.org © 2012 Lymphoma Research Foundation | Privacy Policy

News from the Biological Stain Commission no. 12

DEFF Research Database (Denmark)

Lyon, H O

2012-01-01

In this 12(th) issue of News from the Biological Stain Commission (BSC) under the heading of Regulatory affairs, the Biological Stain Commission's International Affairs Committee presents information from the meetings of ISO/TC 212/WG 1 Quality and competence in the medical laboratory and ISO....../TC 212/WG 3 In vitro diagnostic products both held on 2 - 3 June 2010, plus information on the second plenary meeting of ISO/TC 212 Clinical laboratory testing and in vitro diagnostic test systems held on 4 June 2010. All meetings took place in Seoul, Republic of Korea. Finally, information is provided...

[An analysis on funded theses in the Chinese Journal of Parasitology and Parasitic Diseases in 2009-2012].

Science.gov (United States)

Yi, Feng-yun; Zhang, Zheng-yan; Sheng, Hui-feng

2014-12-01

The published articles of the Chinese Journal of Parasitology and Parasitic Diseases in 2009-2012 (including original articles, experimental researches, field researches and clinical researches) were statistically analyzed. Together 258 research papers were published in the 4 years, and funded papers occupied 82.2% (212/258). The number of papers funded by 1, 2, 3, 4, and 5 foundations projects was 116, 58, 29, 7 and 2, respectively. 61.8% (131/212) of the foundations projects were at the national level; 28.3% (60/212) were at provincial and ministerial level. The papers supported by academy and international agencies accounted for 7.1% (15/212), 2.8% (6/212), respectively. The funded thesis mainly referred to schistosomiasis (35, 16.5%), cystic echinococcosis (29, 13.7%), malaria (24, 11.3%), toxoplasmosis (22, 10.4%), and cysticercosis (9, 4.2%). Five fields covered in these papers were as follows: epidemiology (29, 13.7%), immunology and diagnosis (53, 25%), molecular biology (75, 35.4%), etiology (28,13.2%), and pharmacology (24, 11.3%). The ratio of founded paper was 0.70, 0.67, 0.74, and 0.65 during 2009-2012, respectively. The high ratio of founded paper indicated that this journal is with domestic and abroad importance in the field of parasitology.

Purification of radionuclides for nuclear medicine

International Nuclear Information System (INIS)

Horwitz, E.P.; Bond, A.H.

2002-01-01

Novel systems for the rapid separation of 67 Cu, 90 Y, 188 Re, 212 Bi, 213 Bi, and 223 Ra are described. To achieve the high levels of decontamination required of radionuclides used in radioimmunotherapy, we have utilised combinations of ion exchange (IX) and novel extraction chromatographic (EXC) resins. The objective of each separation system is to remove by many orders of magnitude the parent isotopes and to reduce the concentrations of stable, sometimes adventitious impurities, to low levels. The latter objective is particularly important to achieve a high degree of bonding of radionuclide onto a monoclonal antibody. The purification of 212 Bi (60.6 min half-life) furnishes a good example of the level of purity that we have achieved using tandem combinations of EXC and IX resins. Bismuth-212 was separated from its daughters, 212 Pb, 224 Ra, 228 Th and 232 U at the 50 mCi level. The resultant 212 Bi decayed to background in a few days indicative of high purity. Another example of extraordinary removal of parent nuclide from daughter nuclide and subsequent purification of daughter from stable elements is the preparation of 90 Y. Strontium-90 was removed from 90 Y by > 10 9 . The purified 90 Y uptake by monoclonal antibodies was close to 100%

Coordination chemistry of the {sup 212}Pb/{sup 212}Bi nuclear transformation: Alpha-emitting radiopharmaceuticals. Final technical report

Energy Technology Data Exchange (ETDEWEB)

Parks, N.J.; Harris, W.R.; Keen, C.L.; Cooper, S.R.

1992-07-01

Subdivisions of this project are: (a) the synthesis of prototypical thiolate and dithiocarbamate based hexacoordinate complexes, (b) radiochemical engineering for generation of no-carrier-added lead and bismuth radioelements, (c) the first isolation of bismuth-binding proteins from in vivo studies with cyclotron produced {sup 205/206}Bi tracer, and (d) initial development of transport mechanisms for the intracellular radiobiological study of alpha emitting bismuth, and (e) the initiation of chemical equilibrium studies and biochemical pathways with cyclotron-produced, no-carrier-added, {sup 203}Pb (T{sub 1/2} = 51 hr).

76 FR 9939 - Garnishment of Accounts Containing Federal Benefit Payments

Science.gov (United States)

2011-02-23

... included in the ACH Records. Although the Agencies understand that many financial institutions will make...'' Field is typically captured and included in an account statement, allowing both the financial... 31 CFR 212.5 and 212.6. Financial institutions argued that such a statement would provide certainty...

Reduction of protein adsorption to a solid surface by a coating composed of polymeric micelles with a glass-like core

NARCIS (Netherlands)

Hofs, P.S.; Brzozowska, A.M.; Keizer, de A.; Norde, W.; Cohen Stuart, M.A.

2008-01-01

Adsorption studies by optical reflectometry show that complex coacervate core micelles (C3Ms) composed of poly([4-(2-amino-ethylthio)-butylene] hydrochloride)49-block-poly(ethylene oxide)212 and poly([4-(2-carboxy-ethylthio)-butylene] sodium salt)47-block-poly(ethylene oxide)212 adsorb in equal

Reduction of protein adsorption to a solid surface by a coating composed of polymeric micelles with a glass-like core

NARCIS (Netherlands)

Hofs, B.; Brzozowska, A.; de Keizer, A.; Norde, W.; Stuart, Martien A. Cohen

2008-01-01

Adsorption studies by optical reflectometry show that complex coacervate core micelles (C3Ms) composed of poly([4-(2-amino-ethylthio)-butylene]hydrochloride)(49)-block-poly(ethylene oxide)(212) and poly([4-(2carboxy-ethylthio)-butylene] sodium salt)(47)-block-poly(ethylene oxide)(212) adsorb in

Yeast effects on Pinot noir wine phenolics, color, and tannin composition.

Science.gov (United States)

Carew, Anna L; Smith, Paul; Close, Dugald C; Curtin, Chris; Dambergs, Robert G

2013-10-16

Extraction and stabilization of wine phenolics can be challenging for wine makers. This study examined how yeast choice affected phenolic outcomes in Pinot noir wine. Five yeast treatments were applied in replicated microvinification, and wines were analyzed by UV-visible spectrophotometry. At bottling, yeast treatment Saccharomyces cerevisiae RC212 wine had significantly higher concentrations of total pigment, free anthocyanin, nonbleachable pigment, and total tannin and showed high color density. Some phenolic effects were retained at 6 months' bottle age, and RC212 and S. cerevisae EC1118 wines showed increased mean nonbleachable pigment concentrations. Wine tannin composition analysis showed three treatments were associated with a higher percentage of trihydroxylated subunits (skin tannin indicator). A high degree of tannin polymerization was observed in wines made with RC212 and Torulaspora delbruekii , whereas tannin size by gel permeation chromatography was higher only in the RC212 wines. The results emphasize the importance of yeast strain choice for optimizing Pinot noir wine phenolics.

SPS and PS Experiments Committee

CERN Multimedia

CERN. Geneva

2004-01-01

OPEN SESSION: 09:00 Status report of NA58 / COMPASS: A. Magnon 09:40 Status report of PS212 / DIRAC: L. Tausher 10:10 PS212 / DIRAC Addendum: L. Nemenov CLOSED SESSION on Tuesday, 27 April 2004 after the open session, Main Building, 6th floor conference room

Evaluation of Novel Wet Chemistry Separation and Purification Methods to Facilitate Automation of Astatine-­211 Isolation

Energy Technology Data Exchange (ETDEWEB)

Wilbur, Daniel Scott [Univ. of Washington, Seattle, WA (United States)

2016-07-19

This research is a collaborative effort between the research groups of the PIs, Dr. D. Scott Wilbur in the Department of Radiation Oncology at the University of Washington (UW) and Matthew O’Hara at the Pacific Northwest National Laboratory (PNNL). In this report only those studies conducted at UW and the budget information from UW will be reported. A separate progress and financial report will be provided by PNNL. This final report outlines the experiments (Tasks) conducted and results obtained at UW from July 1, 2013 thru June 30, 2016 (2-­year project with 1 year no-­cost extension). The report divides the information on the experiments and results obtained into the 5 specific objectives of the research efforts and the Tasks within those objectives. This format is used so that it is easy to see what has been accomplished in each area. A brief summary of the major findings from the studies is provided below. Summary of Major Findings from Research/Training Activities at UW: Anion and cation exchange columns did not provide adequate ²¹¹At capture and/or extraction results under conditions studied to warrant further evaluation; PEG-­Merrifield resins containing mPEG350, mPEG750, mPEG2000 and mPEG5000 were synthesized and evaluated; All of the mPEG resins with different sized mPEG moieties conjugated gave similar ²¹¹At capture (>95%) from 8M HCl solutions and release with conc. NH₄OH (~50-­80%), but very low quantities were released when NaOH was used as an eluent; Capture and release of ²¹¹At when loading [²¹¹At]astatate appeared to be similar to that of [²¹¹At]astatide on PEG columns, but further studies need to be conducted to confirm that; Capture of ²¹¹At on PEG columns was lower (e.g. 80-­90%) from solutions of 8M HNO₃, but higher capture rates (e.g. 99%) can be obtained when 10M HNO₃ is mixed with an equal quantity of 8M HCl; Addition of reductants to the ²¹¹At solutions did not appear to change the percent capture, but may have an effect on the % extracted; There was some indication that the PEG-­Merrifield resins could be saturated (perhaps with Bi) resulting in lower capture percentages, but more studies need to be done to confirm that; A target dissolution chamber, designed and built at PNNL, works well with syringe pumps so it can be used in an automated system; Preliminary semi-­automated ²¹¹At isolation studies have been conducted with full-scale target dissolution and ²¹¹At isolation using a PEG column on the Hamilton automated system gave low overall recoveries, but HNO₃ was used (rather than HCl) for loading the ²¹¹At and flow rates were not optimized; Results obtained using PEG columns are high enough to warrant further development on a fully automated system; Results obtained also indicate that additional studies are warranted to evaluate other types of columns for ²¹¹At separation from bismuth, which allow use of HNO₃/HCl mixtures for loading and NaOH for eluting ²¹¹At. Such a column could greatly simplify the overall isolation process and make it easier to automate.

Insights into the structural characteristics and substrate binding analysis of chondroitin AC lyase (PsPL8A) from Pedobacter saltans.

Science.gov (United States)

Rani, Aruna; Dhillon, Arun; Sharma, Kedar; Goyal, Arun

2018-04-01

The structure of chondroitin AC lyase (PsPL8A) of family 8 polysaccharide lyase was characterized. Modeled PsPL8A structure showed, it contains N-terminal (α/α) 6 incomplete toroidal fold and a layered β sandwich structure at C-terminal. Ramchandran plot displayed 98.5% residues in favoured and 1.2% in generously allowed region. Secondary structure of PsPL8A by CD revealed 27.31% α helices 22.7% β sheets and 49.9% random coils. Protein melting study showed, PsPL8A completely unfolds at 60°C. SAXS analysis showed, PsPL8A is fully folded in solution form. The ab initio derived dummy model of PsPL8A superposed well with its modeled structure excluding some α-helices and loop region. Structural superposition and docking analysis showed, N153, W105, H203, Y208, Y212, R266 and E349 were involved in catalysis. Mutants N153A, H203A, Y212F, R266A and E349A created by SDM revealed no residual activity. Isothermal titration calorimetry analysis of Y212F and H203A with C4S polysaccharide, showed moderate binding by Y212F (Ka=9.56±3.81×10 5 ) and no binding with H203A, showing active contribution of Y212 in substrate binding. Residues Y212 and H203 or R266 might act as general base and general acid respectively. Residues N153 and E349 are likely contributing in charge neutralization and stabilizing enolate anion intermediate during β-elimination. Copyright © 2017 Elsevier B.V. All rights reserved.

47 CFR 80.905 - Vessel radio equipment.

Science.gov (United States)

2010-10-01

... the coverage of an INMARSAT maritime mobile geostationary satellite in which continuous alerting is... Relations, USCG Battery Park Building, Room 201, New York, NY 10004-1499. Phone 212-668-7764; Fax 212-668... HF duplex channels; or (B) If operated in an area within the coverage of an INMARSAT maritime mobile...

25 CFR 212.1 - Purpose and scope.

Science.gov (United States)

2010-04-01

..., operations or mining within their territorial jurisdiction. (d) The regulations of the Bureau of Land Management, the Office of Surface Mining Reclamation and Enforcement, and the Minerals Management Service...

36 CFR 212.5 - Road system management.

Science.gov (United States)

2010-07-01

... construction, reconstruction, decommissioning, and maintenance. (2) Identification of unneeded roads... share of the costs. (d) Maintenance and reconstruction of National Forest System roads by users—(1... to cover maintenance or reconstruction of roads shall be used for the purposes deposited, except that...

18 CFR 706.212 - Miscellaneous statutory provisions.

Science.gov (United States)

2010-04-01

...) House Concurrent Resolution 175, 85th Congress, 2d Session, 72 Stat. B12, the Code of Ethics for... reimbursement for travel or other expenses from, or his sale to or purchase of property from a private...

22 CFR 212.1 - Statement of policy.

Science.gov (United States)

2010-04-01

... date. The Director, Office of Administrative Services, Bureau for Management, or his/her designee, is...”), pursuant to executive order and delegations of authority USAID is responsible not only for management of... includes all books, papers, maps, photographs, or other documentary material or copies thereof, regardless...

12 CFR 212.7 - Change in circumstances.

Science.gov (United States)

2010-01-01

... deposits of the depository organization, or an acquisition, merger, consolidation, or reorganization of the ownership structure of a depository organization that causes a previously permissible interlock to become... prohibited. A change in circumstances may include an increase in asset size of an organization, a change in...

Gender | Page 212 | IDRC - International Development Research ...

International Development Research Centre (IDRC) Digital Library (Canada)

Research evidence is supporting the notion that involving women, along with ... The Centre has long valued industry, trade, and commerce as engines of economic growth. From its own practical support for private sector development, IDRC has ... du rôle joué par l'agriculture dans cette ville dynamique d'Afrique de l'Est.

7 CFR 62.212 - Official assessment reports.

Science.gov (United States)

2010-01-01

... Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS INSPECTION ACT (CONTINUED) LIVESTOCK, MEAT, AND OTHER...

7 CFR 980.212 - Import regulations; tomatoes.

Science.gov (United States)

2010-01-01

... the Federal-State Inspection Service, Food Safety and Quality Service, United States Department of.... 33 1 All other points Chief, Fresh Products Branch, Fruit and Vegetable Quality Division, Food Safety... number, the truck and trailer license number, the name of the vessel, or other identification of the...

Calidad de Imagen del Telescopio UNAM212

Science.gov (United States)

Cobos, F. J.; Teiada de Vargas, C.

1987-05-01

El telescopio UNAM2l2, del Observatorio Astronómico Nacional, situado en la Sierra de San Pedro Mártir (Baja California, México), cumplira en un futuro muy cercano siete años de uso para fines de investigación astronómica. Aunque en este tiempo no se ha efectuado un estudio sistemático acerca de su comportamiento óptico y de los factores que influyen en la calidad de las imágenes, se han realizado pruebas diversas, estudios parciales y reuniones especificas, cuyos resultados no siempre se han difundido ampliamente y generalmente no se han presentado por escrito. Es por ello que hemos creido necesario intentar una recopilación de la información existente para poder con ella establecer un diagnóstjco que, aunque no sea definitivo, sirva de base para futuros trabajos tendientes a optimizar el comportamiento óptico del telescopio. Es evidente que un buen número de las conclusiones que se presentan son resultado del trabajo de muchas personas ó de esfuerzos colectivos. Asimismo, hemos tratado de localizar información bibliográfica que pueda ser de utilidad. Nuestro objetivo primordial ha consistido en centrarnos en la óptica del telescopio y su calidad, pero también se han considerado otros aspectos que puedan afectar las imágenes obtenidas tales como: celda del primario, `seeing' local y externo, flexiones posibles en la estructura mecánica del telescopio, etc.

14 CFR 221.212 - Special tariff permission.

Science.gov (United States)

2010-01-01

...) When a filer submits an electronic tariff or an amendment to an electronic tariff for which authority... in the Government Filing File and the Filing Advice Status File, and shall be referenced in such a... notice. (b) When a filer submits an electronic tariff or an amendment to the electronic tariff for which...

34 CFR 668.212 - Loan servicing appeals.

Science.gov (United States)

2010-07-01

...) Any cohort default rate upon which a loss of eligibility under § 668.206 is based. (b) Improper loan... preclaims or default aversion assistance to the guaranty agency; and (ii) Submit a certification or other...

EST Table: FS839039 [KAIKOcDNA[Archive

Lifescience Database Archive (English)

Full Text Available FS839039 E_FL_fner_05J12_F_0 10/09/28 57 %/212 aa gb|ADE18444.1| cytochrome c oxida...se subunit I [Bombyx mori] 10/09/10 50 %/212 aa FBpp0100176|mt:CoI-PA 10/08/29 39 %/207 aa MTCE.26#CE35350#W

Compressive strength of a concrete mix for pavement blocks incorporating industrial by-product

CSIR Research Space (South Africa)

Mokoena, Refiloe

2017-07-01

Full Text Available - reference 3 0 0.26 808 0 1062 344 210 0 - Mix 8 50 0.28 404 404 1062 212 226 0 0 Mix 8A 50 0.28 404 404 1062 212 226 3.10 5 Mix 8B 50 0.28 404 404 1062 212 226 6.21 10 Mix 9 90 0.28 81 727 1062 106 226 0 55 *water:binder ratio where fly ash and cement... was made for the 90% fly ash specimens due to the specimens disintegrating once placed in water. These specimens were therefore cured in heavy duty plastic bags; mixes 3, 6 and 9 were cured in the heavy duty bags before crushing on the appropriate days...

Radiochemical and radioecological studies on Brazilian areas of high natural background. Progress report, October 30, 1974--October 30, 1975

International Nuclear Information System (INIS)

Costa-Ribeiro, C.; Penna-Franca, E.; Rocha-Nogueira, A.; Christian-Pfeiffer, W.

1975-11-01

The absorption of 212 Pb and/or 212 Bi by ertythrocytes was investigated in an attempt to explain the in vivo genesis of somatic chromosomal aberrations of the type detected in peripheral blood lymphocytes of workers professionally exposed to 220 Rn and its decay products, as well as in dwellers of Brazilian areas of high natural radioactivity

Development of an effective face area support system for tabular stopes.

CSIR Research Space (South Africa)

Acheampong, E

2002-03-01

Full Text Available ..................................................................................... 5 2.1.1 Link-bar system ...........................................................................................................5 2.1.2 Crank system... joints would be required for this application. There is no flexibility in this system to allow for various advancing distances. The headboard size would not be able to carry the required loads unless large steel sections were used. 2.1.2 Crank system...

A text in Romani from 1622

DEFF Research Database (Denmark)

Bakker, Peter

2015-01-01

this is a reprint of a 2012 article: A new old text in Romani: Lord's Prayer, 1622. International Journal of Romani Language and Culture 2 (2011): 193-212.......this is a reprint of a 2012 article: A new old text in Romani: Lord's Prayer, 1622. International Journal of Romani Language and Culture 2 (2011): 193-212....

The extent of myocardium at Risk for LAD, RCA and LCx using contrast enhanced SSFP and T2-weighted imaging

DEFF Research Database (Denmark)

Nordlund, D.; Heiberg, E.; Carlsson, Marcus

2015-01-01

in patients after STelevation myocardial infarction. Methods: CE-SSFP and T2w data from the recently published international multi-center trials CHILL-MI and MITOCARE, was used to assess MaR. CESSFP images from 206/212 patients (6 excluded due to inability to detect MaR) and T2w images from 147/212 patients...

The in vivo fate of a 211At labelled monoclonal antibody with known specificity in a murine system

International Nuclear Information System (INIS)

Vaughan, A.T.M.; Bateman, W.J.; Fisher, D.R.

1982-01-01

A monoclonal antibody reactive against the human transferrin receptor has been labelled with the alpha and X ray emitting isotope Astatine 211. The labelling procedure does not affect the ability of the product to bind to the transferrin receptor on the human leukemic cell line HL60. Using a direct binding assay, 211 At labelled antibody can be specifically inhibited from binding to its target cells by excess unlabelled antibody. Furthermore, the binding inhibition demonstrated in this system correlates to enhanced clonogenic survival of these cells, indicating that very few atoms of 211 At/cell are required for cell death. Data obtained from labelled antibody injected into mice show that the labelled product in serum retains the ability to bind to HL60 cells in vitro, although tissue distributions of the injected activity implies that some of the radiolabel is lost from the protein. Despite this loss of label, preliminary experiments on the localization of labelled antibody to HL60 cells growing s/c in nude mice show that tumor tissue has a higher specific activity than all other tissues, other than blood, after 12 hours. This suggests that further work on the nature of label degradation in vivo is warranted in the context of potential therapeutic and diagnostic studies

Training Mothers in Elaborative Reminiscing Enhances Children's Autobiographical Memory and Narrative

Science.gov (United States)

Reese, Elaine; Newcombe, Rhiannon

2007-01-01

This longitudinal intervention assessed children's memory at 2-1/2 years (short-term posttest; N = 115) and their memory and narrative at 3-1/2 years (long-term posttest; N = 100) as a function of maternal training in elaborative reminiscing when children were 1-1/2 to 2-1/2 years. At both posttests, trained mothers were more elaborative in their…

Search for 12 C+ 12 C clustering in 24 Mg ground state

Indian Academy of Sciences (India)

In the backdrop of many models, the heavy cluster structure of the ground state of 24 Mg has been probed experimentally for the first time using the heavy cluster knockout reaction 24 Mg( 12 C, 212 C) 12 C in thequasifree scattering kinematic domain. In the ( 12 C, 212 C) reaction, the direct 12 C-knockout cross-section was ...

Raspberry wine fermentation with suspended and immobilized yeast cells of two strains of Saccharomyces cerevisiae.

Science.gov (United States)

Djordjević, Radovan; Gibson, Brian; Sandell, Mari; de Billerbeck, Gustavo M; Bugarski, Branko; Leskošek-Čukalović, Ida; Vunduk, Jovana; Nikićević, Ninoslav; Nedović, Viktor

2015-01-01

The objectives of this study were to assess the differences in fermentative behaviour of two different strains of Saccharomyces cerevisiae (EC1118 and RC212) and to determine the differences in composition and sensory properties of raspberry wines fermented with immobilized and suspended yeast cells of both strains at 15 °C. Analyses of aroma compounds, glycerol, acetic acid and ethanol, as well as the kinetics of fermentation and a sensory evaluation of the wines, were performed. All fermentations with immobilized yeast cells had a shorter lag phase and faster utilization of sugars and ethanol production than those fermented with suspended cells. Slower fermentation kinetics were observed in all the samples that were fermented with strain RC212 (suspended and immobilized) than in samples fermented with strain EC1118. Significantly higher amounts of acetic acid were detected in all samples fermented with strain RC212 than in those fermented with strain EC1118 (0.282 and 0.602 g/l, respectively). Slightly higher amounts of glycerol were observed in samples fermented with strain EC1118 than in those fermented with strain RC212. Copyright © 2014 John Wiley & Sons, Ltd.

Properties of radioactive aerosols produced by interactions of indoor radon decay products with cigarette smoke and burning cigarettes

International Nuclear Information System (INIS)

Martell, E.A.; Sweder, K.S.

1984-01-01

Risks of lung cancer to smokers, attributable in part to exposure to indoor radon decay products, are dependent on properties of radon progeny-tagged smoke particles. The authors have investigated the properties and interactions of radon progeny-tagged smoke particles as they pass through burning cigarettes into mainstream smoke, using /sup 212/Pb-tagged smoke particles as tracers, cascade impactors for particle size determinations, and low-level β/sup -/ counting techniques. /sup 212/Pb-tagged particles of submicron size are destroyed in the burning zone of cigarettes. However, /sup 212/Pb-tagged smoke particles exceeding 1.0 μm diameter pass readily through the burning zone and tobacco rod into mainstream smoke. /sup 212/ Pb- tagged particles in mainstream smoke have an activity median aerodynamic diameter between 1.0 and 2.0 μm diameter. Particles > 2.0 μm diameter carry about 10 percent of the total activity, are selectively deposited at the carina of bifurcations, and are resistant to dissolution in lung fluid. These results indicate that indoor radon progeny on large particles in mainstream smoke can contribute substantially to the cumulative alpha radiation dose at ''hot spots'' in the bronchi of smokers

Radioactivity in coffee

International Nuclear Information System (INIS)

Roselli, C.; Desideri, D.; Feduzi, L.; Rongoni, A.; Saetta, D.

2013-01-01

This research was dedicated to the study of the background levels of 210 Po and natural gamma emitters as 40 K, 214 Pb, 214 Bi, 228 Ac, 212 Pb and 212 Bi in coffee powder and in coffee beverage; also the artificial 137 Cs was determined. In the coffee powder the mean 210 Po activity resulted 7.25 ± 2.25 x 10 -2 Bq kg -1 . 40 K showed a mean activity of 907.4 ± 115.6 Bq kg -1 . The mean activity concentration of 214 Pb and 214 Bi, indicators of 226 Ra, given as mean value of the two radionuclides, resulted 10.61 ± 4.02 Bq kg -1 . 228 Ac, 228 Ra indicator, showed a mean activity concentration of 13.73 ± 3.20 Bq kg -1 . The mean activity concentration of 212 Pb, 224 Ra indicator, was 8.28 ± 2.88 Bq kg -1 . 208 Tl, 224 Ra indicator, presented a mean activity concentration of 11.03 ± 4.34 Bq kg -1 . In all samples, the artificial 137 Cs resulted below the detection limit (2.0 Bq kg -1 ). The arithmetical mean value of percentage of 210 Po extraction in coffee beverage resulted 20.5 ± 6.9. The percentage of transfer of gamma emitters, 40 K, 214 Pb, 214 Bi, 228 Ac, 212 Pb, 208 Tl resulted of 80.0, 33.5, 24.7, 30.0, 35.1 and 53.5 % for 40 K, 214 Pb, 214 Bi, 228 Ac, 212 Pb and 208 Tl respectively. (author)

46 CFR 193.10-5 - Fire pumps.

Science.gov (United States)

2010-10-01

... Pitot tube pressure of not less than 50 p.s.i. Where 11/2-inch hose is permitted in lieu of 21/2-inch... 21/2-inch hose had been permitted. Where 3/4-inch hose is permitted by Table 193.10-5(a), the Pitot... fire pumps; or (B) Another suitable pump capable of supplying one hose stream at a Pitot tube pressure...

Advancements in Distributed Learning (ADL) Environment in Support of Transformation

Science.gov (United States)

2017-01-01

take a variety of forms, including Task Groups, Workshops, Symposia, Specialists’ Meetings, Lecture Series and Technical Courses . The content of this... MySQL and PHP Apache xAPI Extensive Application Program Interface viii STO-TR-HFM-212 HFM-212 Membership List Dr. Oleksandr BUROV Institute...in Paris, France, initiated a new management infrastructure for collaboration and integration of learning courses and technologies. This ADL effort

FAA Statistical Handbook of Aviation: Calendar Year 1990

Science.gov (United States)

1990-01-01

2 4 3 2 3 1 3 1 2 - Construcciones Aeronautics C212 .......................... 16 16 18 16 19 24 27 28 16 15 Convair CV580/640/600...2,782 3,713 4,948 3,874 1,364 1,745 1,672 1,265 501 291 Construcciones Aeronautics C212 22,574 21.710 23,610 21,643 19,891 24.886 34,252 33.902

Efficacy and acceptability of a new topical skin lotion of sodium cromoglicate (Altoderm) in atopic dermatitis in children aged 2-12 years: a double-blind, randomized, placebo-controlled trial.

Science.gov (United States)

Stainer, R; Matthews, S; Arshad, S H; McDonald, S; Robinson, J; Schapira, C; Foote, K D; Baird-Snell, M; Gregory, T; Pollock, I; Stevens, M T; Edwards, A M

2005-02-01

Atopic dermatitis (AD) is a common inflammatory allergic disease of children. The primary anti-inflammatory therapy is topical steroids. An effective treatment without the topical and systemic adverse effects of corticosteroids would be useful. Topical formulations of sodium cromoglicate have been researched in the past, but without consistent results. We report a trial of a new aqueous skin lotion of sodium cromoglicate (Altoderm) in children with AD. To compare the efficacy, safety and acceptability of Altoderm lotion with a placebo control in the treatment of AD in children. A double-blind, controlled study in which children aged 2-12 years with AD were randomized to 12 weeks of treatment with a lotion containing 4% sodium cromoglicate (Altoderm) or the lotion base. To be included subjects had to have a SCORAD score of > or = 25 and Altoderm and 56 to placebo and all were included in the intention-to-treat analysis. The mean +/- SD SCORAD scores at baseline were 41.0 +/- 9.0 (Altoderm) and 40.4 +/- 8.73 (placebo). These scores were reduced after 12 weeks by 13.2 (36%) with Altoderm and by 7.6 (20%) with placebo. The difference of 5.6 (95% confidence interval 1.0-10.3) is statistically significant (P = 0.018). Diary card symptoms improved with both treatments but the improvement was greater in the Altoderm-treated patients. Topical steroid usage was reduced in both groups and was larger in the Altoderm-treated patients. The differences were statistically significant for the mean of all symptoms, the overall skin condition and use of topical steroids. Those for itching and sleep loss were not. Treatment-related adverse events were reported in 11 subjects (Altoderm seven, placebo four). Most of these referred to irritation, redness and burning at the site of application. There were four reports of erythema and pruritus (Altoderm three, placebo one), and three reports of application site burning (Altoderm two, placebo one). None was reported as severe or very severe

Neutron diffraction and TSDC on Ba1−xUxF2+2x solid electrolytes

DEFF Research Database (Denmark)

Ouwerkerk, M.; Andersen, N. H.; Veldkamp, F. F.

1986-01-01

The defect structure of fluorite-type Ba1−xUxF2+2x solid solutions, which exhibit fast fluoride ion conductivity, has been investigated by quasi-elastic diffuse neutron scattering (QDNS) experiments, and thermally stimulated depolarisation current (TSDC) measurements. A comparison with model...... calculations reveals (212) clusters to dominate the defect structure. From 10 to 400K TSDS spectra reveal for x Conductivity parameters calculated from the latter relaxation compare very well with those...... obtained from a.c. ionic conductivity studies. The low-temperature relaxation at 29.5 K is ascribed to depolarisation steps of (212) clusters. Evidence for the relaxation of a minor amount of (UBaFi)x dipoles is found. A direct indication of the freedom of orientation of a (212) cluster is obtained from...

Air Force Operational Medicine: Using the Estimating Supplies Program to Develop Materiel Solutions for the Operational Clinical Requirements of the Expeditionary Medical Support (EMEDS). Volume Three: EMEDS+10

Science.gov (United States)

2009-11-01

1,480.50 D 7420011828535 CALCULATOR ELECTRONIC SOLAR CELL 8 DIGIT LIQ EA 1.00 0.00 1.15 0.15 $12.63 0.00 0.00 0.00 A 7240000893827 CAN WATER MILITARY PLAS...4110014974328 RECORDER TEMP BLD PLASM FRZER 115V 11.5 CU EA 1.00 1.00 120.00 14.00 $7,212.00 120.00 14.00 $7,212.00 A 4110012494476 REFRIGERATOR

Spawning migration of brown trout, Salmo trutta in the Morávka reservoir

Czech Academy of Sciences Publication Activity Database

Piecuch, J.; Lojkásek, B.; Lusk, Stanislav; Marek, T.

2007-01-01

Roč. 56, č. 2 (2007), s. 201-212 ISSN 0139-7893 R&D Projects: GA AV ČR IAA6093105; GA AV ČR 1QS500450513 Institutional research plan: CEZ:AV0Z60930519 Keywords : reproductive migration * tributary * trap * diel activity * environmental factors Subject RIV: EH - Ecology, Behaviour Impact factor: 0.376, year: 2007 http://www.ivb.cz/folia/56/2/201-212_MS1292.pdf

Sequential culture with Torulaspora delbrueckii and Saccharomyces cerevisiae and management of fermentation temperature to improve cherry wine quality.

Science.gov (United States)

Sun, Shu Yang; Gong, Han Sheng; Zhao, Yu Ping; Liu, Wen Li; Jin, Cheng Wu

2016-04-01

There has been limited research on the use of non-Saccharomyces yeasts for the production of cherry wines. This work used an autochthonous Torulaspora delbrueckii strain 49 (TD49) in association with a commercial S. cerevisiae RC212 yeast, to investigate the effect of multi-starter culture (sequential inoculation and simultaneous inoculation) and fermentation temperature on the quality of cherry wines. Both TD49 and RC212 proliferated during alcoholic fermentation (AF) under sequential inoculation conditions, whereas in the case of simultaneous inoculation, TD49 increased slowly at first and then declined sharply near the fermentation end. The analytical profile showed that both mixed fermentations produced lower levels of volatile acidy and higher levels of aromatic compounds than those from RC212 mono-culture. During sensory analysis, wines from sequential fermentation obtained the highest score, mainly due to the higher intensity in 'fruity' and 'floral' characters. As for the influence of temperature, a low temperature (20 °C) enhanced TD49 persistence during AF, but the sensory quality decreased anyway; 30 °C resulted in decreases in most measured descriptors. Therefore, 25 °C was selected as the best culture temperature. TD49/RC212 sequential inoculation and fermentation at 25 °C significantly enhanced the cherry wine quality. © 2015 Society of Chemical Industry.

Effects of Six Commercial Saccharomyces cerevisiae Strains on Phenolic Attributes, Antioxidant Activity, and Aroma of Kiwifruit (Actinidia deliciosa cv.) Wine

Science.gov (United States)

Li, Xingchen; Cao, Lin; Li, Shaohua; Wang, Ranran; Jiang, Zijing; Che, Zhenming; Lin, Hongbin

2017-01-01

“Hayward” kiwifruit (Actinidia deliciosa cv.), widely planted all around the world, were fermented with six different commercial Saccharomyces cerevisiae strains (BM4×4, RA17, RC212, WLP77, JH-2, and CR476) to reveal their influence on the phenolic profiles, antioxidant activity, and aromatic components. Significant differences in the levels of caffeic acid, protocatechuate, and soluble solid content were found among wines with the six fermented strains. Wines fermented with RC212 strain exhibited the highest total phenolic acids as well as DPPH radical scavenging ability and also had the strongest ability to produce volatile esters. Wines made with S. cerevisiae BM 4×4 had the highest content of volatile acids, while the highest alcohol content was presented in CR476 wines. Scoring spots of wines with these strains were separated in different quadrants on the components of phenolics and aromas by principal component analyses. Kiwifruit wines made with S. cerevisiae RC212 were characterized by a rich fruity flavor, while CR476 strain and WLP77 strain produced floral flavors and green aromas, respectively. Altogether, the results indicated that the use of S. cerevisiae RC212 was the most suitable for the fermentation of kiwifruit wine with desirable characteristics. PMID:28251154

8 CFR 212.1 - Documentary requirements for nonimmigrants.

Science.gov (United States)

2010-01-01

... special humanitarian concern; and (iv) Poses no threat to the welfare, safety or security of the United States, its territories, or commonwealths. Any potential threats to the welfare, safety, or security of...) of this section: Australia, Brunei, Indonesia, Japan, Malaysia, Nauru, New Zealand, Papua New Guinea...

9 CFR 93.212 - Manure from quarantined poultry.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Manure from quarantined poultry. 93... OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS IMPORTATION OF CERTAIN ANIMALS, BIRDS, FISH, AND POULTRY, AND CERTAIN ANIMAL, BIRD, AND POULTRY PRODUCTS...

Dicty_cDB: CHC212 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available ce nqqr*innklrc*hsnn*kkpkkisllmkinselhqamkp*wklsri*kprttefqslkm klml*iyy*mesqkvlkscalavvplmksvsrkfisvmilhsst...c*hsnn*kkpkkisllmkinselhqamkp*wklsri*kprttefqslkm klml*iyy*mesqkvlkscalavvplmksvsrkfisvmilhsstfikrf*lyqlinkl...**itrxk elnnsvyhv--- ---tnkgk*iin*dvniqtikrnrkrfhc**k*iqsctkq*nl*wklsri*kprttefqs lkmklml*iyy*mesqkvlkscalav

40 CFR 156.212 - Personal protective equipment statements.

Science.gov (United States)

2010-07-01

... footwear Shoes Shoes Chemical-resistant gloves 2 Chemical-resistant gloves 2 Chemical-resistant gloves 2 No... and diluted solely with water for application, the glove statement shall specify: “waterproof gloves... specify: “waterproof gloves” instead of the statement in paragraph (f)(4) of this section. (4) Other...

sl(2)-1/2 and the triplet model

International Nuclear Information System (INIS)

Ridout, David

2010-01-01

Conformal field theories with sl(2) -1/2 symmetry are studied with a view to investigating logarithmic structures. Applying the parafermionic coset construction to the non-logarithmic theory, a part of the structure of the triplet model is uncovered. In particular, the coset theory is shown to admit the triplet W-algebra as a chiral algebra. This motivates the introduction of an augmented sl(2) -1/2 -theory for which the corresponding coset theory is precisely the triplet model. This augmentation is envisaged to lead to a precise characterisation of the 'logarithmic lift' of the non-logarithmic sl(2) -1/2 -theory that has been proposed by Lesage et al.

33 CFR 110.212 - Newport Bay Harbor, Calif.

Science.gov (United States)

2010-07-01

... Anchorage C-2. A parallelogram-shaped area, 100 feet wide and 400 feet long, lying 100 feet bayward from and... parallelogram-shaped area, 100 feet wide and 500 feet long, lying 100 feet bayward from and parallel to the... grounds—(1) Temporary Anchorage C-1. Southeast of a line parallel to and 170 feet from the pierhead line...

49 CFR 212.223 - Operating practices compliance inspector.

Science.gov (United States)

2010-10-01

...) Control of Alcohol and Drug Use (49 CFR part 219); (3) Rear End Marking Device Regulations (49 CFR part... regulations listed in paragraph (a) of this section, including the remedial action required to bring defective...

Session 21.2 - Measurement of Light at Night

Science.gov (United States)

Wainscoat, Richard J.

2016-10-01

The introduction of the mercury vapor lamp for general lighting in the 1930s probably marked the beginning of significant light pollution. Lighting levels have increased slowly, year-to-year, with sky brightness levels increasing only slowly on timescales of a year; no measurement protocols or instruments existed to quantify this increase. However, on timescales of 10-20 years, or on generational timescales, the increases in night sky levels, particularly in urban areas, have been dramatic. Younger people speak with their parents or grandparents who remark how beautiful the sky used to be, and how many stars they could see when they when they were younger. Older people can themselves remember how many stars were visible in the sky when they were younger. Whole generations of children now grow up without ever seeing the Milky Way. Society has not had tools to easily measure sky brightness, and monitoring from space has only recently become available. A subtle increase of 10% sky brightness per year, for example, is not noticeable to the human eye on the time scale of a year, and has been tolerated by society. But such an increase compounds to an increase of a factor 2.6 in 10 years, 6.7 in 20 years, and a factor 45 in 40 years, corresponding to a dramatic increase in sky brightness, an almost complete loss in ability to see faint objects in the night sky, and rendering the sky unusable for most forms of astronomy. The most striking examples are the urban observatories found in many major cities that can no longer be used. Session 2 was primarily focused on measurement of light at night, with an emphasis on measurement of light pollution. It comprised of 6 papers that are summarized below. Over the last decade, our ability to measure light pollution has grown tremendously, and the instrumentation needed to produce reliable quantitative measurements has become much more affordable, and now includes consumer grade digital cameras and even smart phones. During this same time period, light pollution has continued to grow. The widespread changes from mostly high-pressure sodium lighting to LED lighting that are now occurring make continued monitoring and measurement of light pollution particularly important into the future. Complete presentations may be viewed at: http://www.noao.edu/education/IAUGA2015FM21

76 FR 212 - Notice and Request for Comments

Science.gov (United States)

2011-01-03

... Federal and State agencies, and industry stakeholders to monitor traffic flows and rate trends in the... submit reports, keep records, or provide information to the agency, third parties, or the public. Under...

212ʻ Fahrenheiti : eesti luule 2006 / Piret Bristol

Index Scriptorium Estoniae

Bristol, Piret, 1968-

2008-01-01

Järgmistest 2006. a. ilmunud luulekogudest: Ryytle, Indrek. Inglid rokijaamas. Puhja : I. Rüütle ; Runnel, Hando. Viru veri ei värise. Tartu : Ilmamaa ; Vabat, Martin. Mina olengi kirjandusklassik. Tartu : Eesti Kirjanduse Selts ; Korts, Eliina. Lööklaused murravad metsi. Tartu : Eesti Kirjanduse Selts ; Koff, Indrek. Vana laul. Luige : Verb ; Teede, Andra. Takso Tallinna taevas. Luige : Verb ; Vusser, Kaspar. Vusserduste võnked. Tallinn : P. Kukk ; Elbing, Andrus. Siin Beebilõust, tere! : häired pimelinna tänavalt = Ciao, it's Babyface : trouble from the streets of a blind city. Tallinn : Epifanio ; Hint, Miina. Vabaduse vang, ehk, Põrandaalune kirjandus. Tallinn : MR ; Jüriado, Tiiu. Luule on ime. Habaja : Kentaur ; Ligi, Katre. Naabrivalve. Tartu : Ilmamaa ; Krull, Hasso. Talv. Tallinn : Tuum ; Mets, Mae. Pühapäeval on reede. Tartu : Ilmamaa ; Ploom, Ülar. Porr ja sorry. Tallinn : Tuum ; Hirv, Indrek, Surmapõletaja. Tallinn : Tuum ; Piir, Milvi Martina. Kõrkjavaas. Tartu : Fantaasia ; Soomets, Triin. Väljas. Tallinn : Tuum ; Mathura. Kohalolu. Rapla : Allikäärne ; Afanasjev, Vahur. Katedraal Emajões. Tartu ; Brüssel : ID Salong//Sild, Ivar. Spermaga ja puha. Pärnu : Jumalikud Ilmutused ; Meiel, Kaupo. Polügrafisti käsiraamat. Pärnu : Jumalikud Ilmutused ; Kivisildnik, Sven. Vägistatud jäämägi. Pärnu : Jumalikud Ilmutused ; Arder, Ott. Luule sünnib kus sünnib kui sünnib / koost. Leelo Tungal. Tallinn : Tänapäev ; Kompus, Marko. Vallaliste jõgede tõkkejooksja. Tartu : M. Kompus

Dicty_cDB: VHL212 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available anslated Amino Acid sequence n*iahvq*kigalsgpflvsrtgdvg*tkywdktsnih**ipqkvlvh*dsrtvamevgi r*gvcnnspakwtspeng...plnss ivddyqxtrccklpssvqlrgvrlfh Frame B: n*iahvq*kigalsgpflvsrtgdvg*tkywdktsnih**ipqkvlvh*dsrtvamevgi r*gvcnns

Draft genome sequence of Coxiella burnetii Dog Utad, a strain isolated from a dog-related outbreak of Q fever

Directory of Open Access Journals (Sweden)

F. D’amato

2014-07-01

Full Text Available Coxiella burnetii Dog Utad, with a 2 008 938 bp genome is a strain isolated from a parturient dog responsible for a human familial outbreak of acute Q fever in Nova Scotia, Canada. Its genotype, determined by multispacer typing, is 21; the only one found in Canada that includes Q212, which causes endocarditis. Only 107 single nucleotide polymorphisms and 16 INDELs differed from Q212, suggesting a recent clonal radiation.

Strategies to monitor non-homogeneous atmospheres in sealed off panels in coal mines.

CSIR Research Space (South Africa)

Hardman, DR

2001-06-01

Full Text Available of international practice 7 2.1 Monitoring of atmospheres in inaccessible areas of 8 coal mines - practices in Europe and North America 2.1.1 Introduction 8 2.1.2 Monitoring techniques 9 2.1.3 Monitoring... 27 4.2 Currently available technologies 28 4.3 Alternative technologies 29 5 Conclusions and recommendations 29 6 References 30 6 List of figures Page Figure 2.1.2 Schematic of tube bundle...

The radiolabeled monoclonal antibodies in immunoscintigraphy and radioimmunotherapy: current state and perspectives

International Nuclear Information System (INIS)

Chatal, J. F.

2000-01-01

The antibodies can be satisfactorily labelled with technitium-99 m or indium-111 for tumor immunoscintigraphy. The immunoscintigraphy is not useful for the primary tumor diagnosis. It can be useful for the diagnosis of the some cancer extension and for recurrent tumor visualization. The immunoscintigraphy is widely competed with Positron Emission Tomography (PET) which gives accurate results. In the future the immunoscintigraphy, in pre-therapeutic stage, contribute to the estimation of the dose delivered to the tumor and to normal organs for adopting or not a radioimmunotherapy. The antibodies can also be labeled with Iodine-131 for an application in radioimmunotherapy (RIT). The RIT is efficient in the non-Hodgkin's lymphoma treatment because of their great radiosensitivity. Until now the results have been very modest in solid tumor treatment but methodological and biotechnological progresses have to improve the efficiency especially for the small tumors. In the future iodine-131 which requires the confinement (very expensive) of patients will be substituted by yttrium-90 beta emitter, more energetic than iodine-131 and can be injected in walking case. In the long term, the alpha emitter radionuclides (astatine-211 or bismuth-213) can be used for hematologic cancer treatment. In conclusion the future of radiolabeled monoclonal antibodies is essentially therapeutic. The radioimmunotherapy associated to the chemotherapy give promising perspectives for the radiosensitive cancer treatment and in general small solid tumor treatment (F.M.)

On the theory of time dilation in chemical kinetics

Science.gov (United States)

Baig, Mirza Wasif

2017-10-01

The rates of chemical reactions are not absolute but their magnitude depends upon the relative speeds of the moving observers. This has been proved by unifying basic theories of chemical kinetics, which are transition state theory, collision theory, RRKM and Marcus theory, with the special theory of relativity. Boltzmann constant and energy spacing between permitted quantum levels of molecules are quantum mechanically proved to be Lorentz variant. The relativistic statistical thermodynamics has been developed to explain quasi-equilibrium existing between reactants and activated complex. The newly formulated Lorentz transformation of the rate constant from Arrhenius equation, of the collision frequency and of the Eyring and Marcus equations renders the rate of reaction to be Lorentz variant. For a moving observer moving at fractions of the speed of light along the reaction coordinate, the transition state possess less kinetic energy to sweep translation over it. This results in the slower transformation of reactants into products and in a stretched time frame for the chemical reaction to complete. Lorentz transformation of the half-life equation explains time dilation of the half-life period of chemical reactions and proves special theory of relativity and presents theory in accord with each other. To demonstrate the effectiveness of the present theory, the enzymatic reaction of methylamine dehydrogenase and radioactive disintegration of Astatine into Bismuth are considered as numerical examples.

Silver impregnated nanoparticles of titanium dioxide as carriers for {sup 211}At

Energy Technology Data Exchange (ETDEWEB)

Cedrowska, Edyta; Lyczko, Monika; Piotrowska, Agata; Bilewicz, Aleksander [Institute of Nuclear Chemistry and Technology, Warsaw (Poland); Stolarz, Anna; Trcinska, Agnieszka [Warsaw Univ. (Poland). Heavy Ion Lab.; Szkliniarz, Katarzyna [Silesia Univ. Katowice (Poland). Inst. of Physics; Was, Bogdan [Polish Academy of Science, Cracow (Poland). Inst. of Nuclear Physics

2016-08-01

The {sup 211}At radioisotope exhibits very attractive nuclear properties for application in radionuclide therapy. Unfortunately use of {sup 211}At is limited, because astatine as the heaviest halogen forms weak bond with carbon atoms in the biomolecules which makes {sup 211}At bioconjugates unstable in physiological conditions. In our work we propose a new solution for binding of {sup 211}At which consists of using nanoparticles of titanium dioxide modified with silver atoms as carriers for {sup 211}At. Ag{sup +} cations have been absorbed on the nanometer-sized TiO{sub 2} particles (15 and 32 nm) through ion exchange process and were reduced in Tollens' reaction. The obtained TiO{sub 2}-Ag nanoparticles were labeled with {sup 211}At. It was found that labeling yields were almost quantitative under reducing conditions, while under oxidizing conditions they dropped to about 80%. The labeled nanoparticles exhibited very high stability in physiological salt, PBS buffer, solutions of peptides (0.001 M cysteine, 0.001 M glutathione) and in human blood serum. To make TiO{sub 2}/Ag nanoparticles well dispersed in water and biocompatible their surface was modified with a silane coupling agent containing poly(ethyleneglycol) molecules. The developed functionalization approach will allow us to attach biomolecules to the TiO{sub 2}/Ag surface.

Radiobromination of humanized anti-HER2 monoclonal antibody trastuzumab using N-succinimidyl 5-bromo-3-pyridinecarboxylate, a potential label for immunoPET

Energy Technology Data Exchange (ETDEWEB)

Mume, Eskender [Organic Chemistry, Department of Chemistry, Uppsala University, S-751 24 Uppsala (Sweden); Orlova, Anna [Affibody AB, S-161 02 Bromma (Sweden); Malmstroem, Per-Uno [Division of Urology, Department of Surgical Sciences, Uppsala University, S-751 85 Uppsala (Sweden); Lundqvist, Hans [Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, S-751 85 Uppsala (Sweden); Sjoeberg, Stefan [Organic Chemistry, Department of Chemistry, Uppsala University, S-751 24 Uppsala (Sweden); Tolmachev, Vladimir [Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, S-751 85 Uppsala (Sweden)]. E-mail: vladimir.tolmachev@bms.uu.se

2005-08-01

Combining the specificity of radioimmunoscintigraphy and the high sensitivity of PET in an in vivo detection technique could improve the quality of nuclear diagnostics. Positron-emitting nuclide {sup 76}Br (T {sub 1/2}=16.2 h) might be a possible candidate for labeling monoclonal antibodies (mAbs) and their fragments, provided that the appropriate labeling chemistry has been established. For internalizing antibodies, such as the humanized anti-HER2 monoclonal antibody, trastuzumab, radiobromine label should be residualizing, i.e., ensuring that radiocatabolites are trapped intracellularly after the proteolytic degradation of antibody. This study evaluated the chemistry of indirect radiobromination of trastuzumab using N-succinimidyl 5-(tributylstannyl)-3-pyridinecarboxylate. Literature data indicated that the use of this method provided residualizing properties for iodine and astatine labels on some antibodies. An optimized 'one-pot' procedure produced an overall labeling efficiency of 45.5{+-}1.2% over 15 min. The bromine label was stable under physiological and denaturing conditions. The labeled trastuzumab retained its capacity to bind specifically to HER2-expressing SKOV-3 ovarian carcinoma cells in vitro (immunoreactivity more than 75%). However, in vitro cell test did not demonstrate that the radiobromination of trastuzumab using N-succinimidyl 5-bromo-3-pyridinecarboxylate improves cellular retention of radioactivity in comparison with the use of N-succinimidyl 4-bromobenzoate.

Precise measurement of the absolute yield of fluorescence photons in atmospheric gases

Czech Academy of Sciences Publication Activity Database

Ave, M.; Boháčová, Martina; Daumiller, K.; Di Carlo, P.; Di Giulio, C.; San Luis, P.F.; Gonzales, D.; Hojvat, C.; Hörandel, J.R.; Hrabovský, M.; Iarlori, M.; Keilhauer, B.; Klages, H.; Kleifges, M.; Kuehn, F.; Monasor, M.; Nožka, Libor; Palatka, Miroslav; Petrera, S.; Privitera, P.; Řídký, Jan; Rizi, V.; d´Orfeuil, B.R.; Salamida, F.; Schovánek, Petr; Šmída, Radomír; Spinka, H.; Ulrich, A.; Verzi, V.; Williams, C.

212-213, - (2011), s. 356-361 ISSN 0920-5632 R&D Projects: GA MŠk LC527; GA MŠk(CZ) 1M06002; GA MŠk(CZ) LA08016; GA AV ČR KJB100100904 Institutional research plan: CEZ:AV0Z10100502; CEZ:AV0Z10100522 Keywords : airfly * fluorescence photons * ultra-high energy cosmic rays Subject RIV: BF - Elementary Particles and High Energy Physics http://particle.astro.ru.nl/pub/NuclPhysB212-356.pdf

A study on thoron decay products in the air

Energy Technology Data Exchange (ETDEWEB)

Ahmed, A A; Abul-Hussein, A; Mahmoud, M [Physics Dept. Faculty of Science, El-minia Univ., El-Minia (Egypt)

1997-12-31

Low pressure Berner cascade impactor technique was used as sampling device to determine the concentration ansd activity size distribution of {sup 212}Pb in the open air. The collected activity in each stage of the impactor was measured by a {gamma} spectrometer with 5 x 5 Nal(tl)detector. The activity size distribution of {sup 212}Pb was described by one long-normal distribution. The mean AMAD was 345 nm with {alpha}{sub 9}=2.7 o. 4 figs., 3 tabs.

Future Research Themes in Supply Chain Management

DEFF Research Database (Denmark)

Wieland, Andreas

2016-01-01

Guest post on research results published in the article "Mapping the Landscape of Future Research Themes in Supply Chain Management" by Andreas Wieland, Robert Handfield and Christian Durach ( Journal of Business Logistics (2016). Vol. 37, no. 3, pp. 205-212).......Guest post on research results published in the article "Mapping the Landscape of Future Research Themes in Supply Chain Management" by Andreas Wieland, Robert Handfield and Christian Durach ( Journal of Business Logistics (2016). Vol. 37, no. 3, pp. 205-212)....

Late quaternary hillslope evolution recorded in eastern South African colluvial badlands

CSIR Research Space (South Africa)

Clarke, ML

2003-09-01

Full Text Available -8-03 Palaeogeography, Palaeoclimatology, Palaeoecology 197 (2003) 199^212 www.elsevier.com/locate/palaeo dates for calcrete nodules which formed post-dep- ositionally within the sequences and which could not constrain individual depositional or pedogenic events. As a..., the earlier ones of which correlate with the calculated changes in latitudinal insolation. As identi?ed by Thomas and Thorp (1995), the PALAEO 3146 4-8-03 M.L. Clarke et al. / Palaeogeography, Palaeoclimatology, Palaeoecology 197 (2003) 199^212 timing...

Census of U.S. Civil Aircraft, Calendar Year 1980.

Science.gov (United States)

1980-12-31

LA-4 4 41 UNKNOWN 1 0 0 33 33 F/W S-EN REC. END 41 0 268 26 TOTAL 0 206 206 CONSTRUCCIONES AERONAATICAS SA 1.131 2 41 UNKNOWN 1 0 0 10 10 F/W S-EWE...52 0 14 145 F/W MULTI TURBOJET 54 3 508 TOTAL 5 643 354 CONSTRUCCIONES AERONAUTICAS SA C-212-CB 19 52 AIRE 2 776 0 2 2 C-212-CB 19 52 UNKNOWN 2 0 1 2

Magnetostrictive and mechanical properties of Terfenol-D composites based on polymer

Science.gov (United States)

Rodríguez, C.; Cuevas, J. M.; Orue, I.; Vilas, J. L.; Barandiarán, J. M.; Fernandez-Gubieda, M. L.; Leon, L. M.

2007-07-01

Several composites, with outstanding magnetostrictive properties, have been synthesized combining a polyurethane base elastomer, with polycrystalline powders of Terfenol-D with a preferential orientation obtained by curing the material in a magnetic field. The morphology of the polymer matrix can be modified by changing the ratio of the hard /soft segment (F) of the polyurethane from 0.6 to 1.5. The influence of the morphology in the magnetostrictive response, for different composites, has been studied by following the storage modulus, E', in DMTA analysis. The magnetostrictive response has been studied as a function of Terfenol-D particle size and distribution (0-300, 212-300, 106-212, 0-38 μm), as well as a function of the content of the magnetostrictive particles in the composite. The highest response (about 1390 ppm) was obtained for a F=1.5 polyurethane and 50% wt of Terfenol-D of 212-300 μm particle size, oriented with a magnetic field of 0.5 T.

Mechanism of the Ca2+-induced enhancement of the intrinsic factor VIIa activity

DEFF Research Database (Denmark)

Bjelke, Jais R; Olsen, Ole H; Fodje, Michel

2008-01-01

between the loop and Lys(161) in the N-terminal tail. In support of the first mechanism, the mutations E296V and D212N resulted in similar, about 2-fold, enhancements of the amidolytic activity. Moreover, mutation of the Lys(161)-interactive residue Asp(217) or Asp(219) to Ala reduced the amidolytic...... activity by 40-50%, whereas the K161A mutation resulted in 80% reduction. Hence one of these Asp residues in the Ca(2+)-binding loop appears to suffice for some residual interaction with Lys(161), whereas the more severe effect upon replacement of Lys(161) is due to abrogation of the interaction with the N......-terminal tail. However, Ca(2+) attenuation of the repulsion between Asp(212) and Glu(296) keeps the activity above that of apoFVIIa. Altogether, our data suggest that repulsion involving Asp(212) in the Ca(2+)-binding loop suppresses FVIIa activity and that optimal activity requires a favorable interaction...

Inertial Navigation System Aiding Using Vision

Science.gov (United States)

2013-03-01

abp a + Cba d dt ( pa ) + d dt ( rbba ) (2.11) vb = d dt ( rbba ) + Cba (Ω a... abp a + va) (2.12) where ddt (r b ba) accounts for the relative velocity betwwen the a-frame and b-frame, CbaΩaabp a is the instantaneous velocity of p...frame. Taking another time derivative of Eq. 2.12 results in: d dt ( vb ) , ab = d2 dt2 rbba + d dt [ Cba (Ω a abp a + va) ] (2.13) = r̈bba + dCba

Production of heavy element and search for new isotopes at JAERI-RMS

Energy Technology Data Exchange (ETDEWEB)

Ikuta, Tomohiko [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

1997-07-01

The new neutron deficient isotope {sup 209}Th and {sup 212}Pa have been produced in heavy ion induced fusion evaporation reactions. The evaporation residues were separated in-flight by the JAERI recoil mass separator (JAERI-RMS). The {alpha}-decay energy of {sup 209}Th and {sup 212}Pa are 8.080(50) MeV and 8.270(30) MeV, respectively. The corresponding half-lives are 3.8{sub -1.5}{sup +6.9} ms and 5.1{sub -1.9}{sup +6.1} ms. (author)

Age determination and feeding habits of Nemipterus japonicus (Bloch, 1791) in the northern Oman Sea

OpenAIRE

Afshari, M.; Valinassab, T.; Seifabadi, J.; Kamaly, E.

2013-01-01

Age determination and feeding habits of the Japanese threadfin bream, Nemipterus japonicus, was carried out in the northern Oman Sea (Chabahar area), based on 212 specimens collected between September 2009 and May 2010. The minimum and maximum fork length and body weight were measured as 145, 258 mm and 55.31, 288.12 g. The relationship between Body Weight (BW) and Fork Length (FL) for all individuals was estimated as BW= 0.0001×FL2.83 (r2 = 0.9425, n= 212). The Vacuity Index (VI) was 55.2% t...

Occupational Survey Report. Food Service Career Ladder AFSC 622X0

Science.gov (United States)

1980-09-01

FRY MEATS, SEAFOODS, OR POULTRY 47 G212 COOK CEREALS SUCH AS OATMEAL OR CORNMEAL 47 G209 BRAISE MEATS, SEAFOODS, OR POULTRY 47 G253 PREPARE MEATS...OATMEAL OR CORNMEAL 62 G210 BREW COFFEE OR TEA 62 G259 PREPARE SANDWICHES 61 G245 PREPARE GARNISHES 61 G260 PREPARE SOUPS 61 1341 CLEAN WORK TABLES 61 H288...EGGS FOR COOKING 80 G260 PREPARE SOUPS 80 G257 PREPARE RICE OR PASTA 80 G212 COOK CEREALS, SUCH AS OATMEAL OR CORNMEAL 77 H288 PREPARE GRIDDLES FOR USE

Constructing Cost-Effective and Targetable ICS Honeypots Suited for Production Networks

Science.gov (United States)

2015-03-26

made one request. |_http -drupal -modules: ERROR: Script execution failed (use -d to debug) 47 |_http -google -malware: [ERROR] No API key found...34________ ") | traceroute - geolocation : | HOP RTT ADDRESS GEOLOCATION |_ 1 2.12 172.16.0.106 - ,- TRACEROUTE HOP RTT ADDRESS 1 2.12 ms 172.16.0.106 Read data...MD5: D9B704524A6DBEC6E55F47CC295BBB3A |_http -google -malware: [ERROR] No API key found. Update the variable APIKEY in http -google -malware or set it

ORF Alignment: NC_004757 [GENIUS II[Archive

Lifescience Database Archive (English)

Full Text Available ... Length = 212 ... Query: 189 LSGKRVLVTAGPTFEALDAVRGLTNLSSGKTXXXXXXXXXXXXXXVTLVCGPVCPPFPAV 248 ... LSGKRVLVTAGPTFE...ALDAVRGLTNLSSGKT ... VTLVCGPVCPPFPAV Sbjct: 1 ... LSGKRVLVTAGPTFE

EMPHASIS™/Nevada CABANA User Guide Version 2.1.2

Energy Technology Data Exchange (ETDEWEB)

Turner, C. David [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Bohnhoff, William J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Powell, Jennifer L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

2017-11-15

The CABle ANAlysis (CABANA) portion of the EMPHASIS™ suite is designed specifically for the simulation of cable SGEMP. The code can be used to evaluate the response of a specific cable design to threat or to compare and minimize the relative response of difference designs. This document provides user-specific information to facilitate the application of the code to cables of interest.

14 CFR 212.6 - Operation of gambling junket charters.

Science.gov (United States)

2010-01-01

... carrier must meet the following criteria: (a) The aircraft must be chartered by (1) A casino, hotel, or..., or by a foreign government, or (2) An agent of such a casino, or cruise line on behalf of that casino, hotel, or cruise line. (b) The casino, hotel, or cruise line or its agents, may not require a passenger...

All projects related to | Page 212 | IDRC - International Development ...

International Development Research Centre (IDRC) Digital Library (Canada)

... lack of basic services, unplanned built environments, and relatively weak governments. ... PLANNING, RISK MANAGEMENT, GENDER ANALYSIS, SOUTHEAST ASIA ... Upgrading Women's Food Product Value Chains in Northern Ethiopia.

Free radical detection by means of 212Pb mirrors

International Nuclear Information System (INIS)

Molera, M.J.; Gamboa, J. M.; Garcia Guijarro, E.

1961-01-01

Radicals have been detected at low temperatures (550-700 degree centigrade) in the thermal decomposition of dimethyl and methyl-ethyl-ketone by the Paneth method. the sensitivity of the method has been increased by the use of radioactive mirrors of PB 2 12. They have proved to detect one radical among 2.10 6 molecules. (Author) 18 refs

26 CFR 1.212-1 - Nontrade or nonbusiness expenses.

Science.gov (United States)

2010-04-01

..., expenses of carrying on transactions which do not constitute a trade or business of the taxpayer and are... the estate or trust is not engaged in a trade or business, except to the extent that such expenses are... in the case of a business expense. [T.D. 6500, 25 FR 11402, Nov. 26, 1960; 25 FR 14021, Dec. 12, 1960...

EMPHASIS™/Nevada UTDEM User Guide Version 2.1.2

Energy Technology Data Exchange (ETDEWEB)

Turner, C. David [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Pasik, Michael F. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Seidel, David B. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Pointon, Timothy D. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Cartwright, Keith L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Kramer, Richard M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); McGregor, Duncan A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

2017-11-15

The Unstructured Time-Domain ElectroMagnetics (UTDEM) portion of the EMPHASIS suite solves Maxwell’s equations using finite-element techniques on unstructured meshes. This document provides user-specific information to facilitate the use of the code for applications of interest.

29 CFR 1910.212 - General requirements for all machines.

Science.gov (United States)

2010-07-01

... be such that it does not offer an accident hazard in itself. (3) Point of operation guarding. (i) Point of operation is the area on a machine where work is actually performed upon the material being... absence of applicable specific standards, shall be so designed and constructed as to prevent the operator...

Coordinating the Structural Rearrangements Associated with Unidirectional Proton Transfer in the Bacteriorhodopsin Photocycle Induced by Deprotonation of the Proton-Release Group: A Time-Resolved Difference FTIR Spectroscopic Study†

Science.gov (United States)

Morgan, Joel E.; Vakkasoglu, Ahmet S.; Lanyi, Janos K.; Gennis, Robert B.; Maeda, Akio

2014-01-01

In the photocycle of bacteriorhodopsin at pH 7, proton release from the proton releasing group (PRG) to the extracellular medium occurs during formation of the M intermediate. This proton release is inhibited at acidic pH, below the pKa of the PRG, ∼6 in M, and instead occurs later in the cycle as the initial state is restored from the O intermediate. Here, structural changes related to deprotonation of the PRG have been investigated by time-resolved FTIR spectroscopy at 25°C. The vibrational features at 2100-1790 cm-1, 1730-1685 cm-1, 1661 cm-1, and 1130-1045 cm-1 have greater negative intensity in the pure M-minus-BR spectrum and even in the M-minus-BR spectrum, that is present earlier together with the L-minus-BR spectrum, at pH 7, than in the corresponding M-minus-BR spectra at pH 5 or pH 4. The D212N mutation abolishes the decreases in the intensities of the broad feature between 1730 and 1685 cm-1 and the band at 1661 cm-1. The 1730-1685 cm-1 feature may arise from transition dipole coupling of the backbone carbonyl groups of Glu204, Phe208, Asp212 and Lys216 interacting with Tyr57 and C15-H of the chromophore. The 1661 cm-1 band, which is insensitive to D2O substitution, may arise by interaction of the backbone carbonyl of Asp212 with C15-H. The 2100-1790 cm-1 feature with a trough at 1885 cm-1 could be due to a water cluster. Depletion of these bands upon deprotonation of the PRG is attributable to disruption of a coordinated structure, held in place by interactions of Asp212. Deprotonation of the PRG is accompanied also by disruption of the interaction of the water molecule near Arg82. The liberated Asp212 may stabilize the protonated state of Asp85, and thus confer uni-directionality to the transport. PMID:20232848

The determination of airborne concentrations of radon and thoron progeny by repetitive alpha counting of filter samples

International Nuclear Information System (INIS)

French, Clayton S. Jr.; Skrable, Kenneth W.; Chabot, George E.

1978-01-01

Analytical equations have been used to determine the airborne concentrations of the particulate daughters of radon and thoron from five net alpha counts obtained at preset time intervals post sampling. The same expressions were used to propagate the associated standard deviations. These propagated errors were minimized by the selection of optimum sampling and counting intervals. An extensive error analysis examined sources of interference and their influence on the calculated concentrations. This system offers sufficient precision for research applications, yet is simple and inexpensive enough for application in field studies. The radon and thoron daughters measured with this technique are 218 Po, 214 Pb, 214 Bi, 212 Pb, and 212 Bi. Because of the decay kinetics involved, the calculated concentrations of 218 Po and 212 Bi involve the greatest uncertainty. The proper choice of sampling and counting intervals optimizes the system for any one of the above radionuclides or for all five collectively. A sampling time of 15 minutes is best for the simultaneous estimation of all five concentrations. Millipore filter samples were obtained from a large, unventilated sub-basement of the University of Lowell research reactor facility and were counted later in direct contact with the window of a gas flow proportional detector having alpha particle counting efficiencies near 0.4 ca -1 and an alpha background of about 1 c min -1 . A typical 15 minute sample obtained at a flow rate of 2 x 10 4 cm 3 min -1 yielded estimates of the airborne concentrations and relative standard deviations: 218 Po, 4.75 x 10 -9 μCi cm -3 ± 18.9%; 214 Pb, 5.15 x 10 -9 μCi cm -3 ± 2.5%; 214 Bi, 4.86 x 10 -9 μCi cm -3 ± 2.4%; 212 Pb, 1.41 x 10 -10 μCi cm -3 ± 2.0%; and 212 Bi, 2.15 x 10 -10 μCi cm -3 ± 27.0%. (author)

Therapy with high LET Radioisotopes: Can sufficient levels of attractive Auger and alpha emitters be produced to make their use practical?

International Nuclear Information System (INIS)

Knapp, F.F. Jr.; Mirzadeh, S.; Stabin, M.; Brill, A.B.

2005-01-01

Because of localized energy deposition within a very small volume, cellular targeted therapy with high linear energy transfer (LET) Auger-electron and alpha-particle emitting radioisotopes is of great interest. While the energy deposition from alpha particles usually encompasses several cell diameters, the dose from Auger electrons is confined to a single cell. Two major challenges for broader use of Alpha and Auger emitters are the efficient and cost effective routine production of sufficient levels of these radioisotopes, and the availability of targeting molecules to which the radioisotopes can be attached for cellular delivery of sufficient levels of activity for effective therapy. Examples of several Alpha-and Auger-emitting radioisotopes of current interest are presented. Alpha- and Auger electron-emitting radioisotopes can be produced in accelerators (A) and nuclear reactors (R), and several alpha emitter congeners (i.e. thorium-229) can be obtained from uranium decay products. The challenge for reactor production, is the availability and exploitation of methods - other then the usual radiative (n,γ) production route which will provide no-carrier-added (nca) or the high specific activity radioisotopes of interest. The submitted manuscript has been authored by a contractor of the U.S. Government under contract DE-AC05-00OR22725. Accordingly, the U.S. Government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes High LET radioisotopes of current interest which are in the initial stages of development and/or which demonstrate practical use in clinical trials include several alpha-emitters, in particular bismuth-213 - and also the actinium-225 parent - astatine-211 and bismuth-212. Extensive experimental studies have been reported with Auger iododeoxyuridine (IdUR) radiolabeled with the iodine-125 emitter-labeled. However, development of production

Effect of cetuximab in combination with alpha-radioimmunotherapy in cultured squamous cell carcinomas

Energy Technology Data Exchange (ETDEWEB)

Nestor, Marika, E-mail: marika.nestor@bms.uu.s [Unit of Otolaryngology and Head and Neck Surgery, Department of Surgical Sciences, Uppsala University, S-751 85 Uppsala (Sweden); Unit of Biomedical Radiation Sciences, Department of Oncology, Radiology and Clinical Immunology, Uppsala University, S-751 85 Uppsala (Sweden); Sundstroem, Magnus [Unit of Molecular Pathology, Department of Genetics and Pathology, Uppsala University (Sweden); Anniko, Matti [Unit of Otolaryngology and Head and Neck Surgery, Department of Surgical Sciences, Uppsala University, S-751 85 Uppsala (Sweden); Tolmachev, Vladimir [Unit of Biomedical Radiation Sciences, Department of Oncology, Radiology and Clinical Immunology, Uppsala University, S-751 85 Uppsala (Sweden)

2011-01-15

Aim: The monoclonal antibody cetuximab, targeting the epidermal growth factor receptor (EGFR), is a promising molecular targeting agent to be used in combination with radiation for anticancer therapy. In this study, effects of cetuximab in combination with alpha-emitting radioimmunotherapy (RIT) in a panel of cultured human squamous cell carcinomas (SCCs) were assessed. Methods: SCC cell lines were characterized and treated with cetuximab in combination with anti-CD44v6 RIT using the astatinated chimeric monoclonal antibody U36 ({sup 211}At-cMAb U36). Effects on {sup 211}At-cMAb U36 uptake, internalization and cell proliferation were then assessed in SCC cells. Results: Cetuximab in combination with {sup 211}At-cMAb U36 mediated increased growth inhibition compared to RIT or cetuximab alone in two cell lines. However, cetuximab also mediated radioprotective effects compared to RIT alone in two cell lines. The radioprotective effects occurred in the cell lines in which cetuximab clearly inhibited cell growth during radiation exposure. Cetuximab treatment also influenced {sup 211}At-cMAb-U36 uptake and internalization, suggesting interactions between CD44v6 and EGFR. Conclusions: Results from this study demonstrate the vast importance of further clarifying the mechanisms of cetuximab and radiation response, and the relationship between EGFR and suitable RIT targets. This is important not only in order to avoid potential radioprotective effects, but also in order to find and utilize potential synergistic effects from these combinations.

Development and radiotherapeutic application of 211At-labeled radiopharmaceuticals. Progress report, March 1, 1981-February 28, 1982

International Nuclear Information System (INIS)

Adelstein, S.J.; Zalutsky, M.; Bloomer, W.

1981-01-01

This project is concerned with developing the potential of alpha-emitting radionuclides as agents for radiotherapy. Alpha-emitters seem ideally suited for his application because their high linear energy transfer and short range permit the deposition of considerable energy in a very small volume of tissue. Unlike the beta particles of 131 I which have a range of about 1 to 2 mm in tissue, 5 to 7 MeV alpha particles would traverse only a few cell diameters. Among the available alpha-emitters, 211 At appears most promising for therapeutic applications because, (1) it has some chemical similarities to iodine, an element that can readily be incorporated into numerous proteins and peptides, (2) it has a half-life that is long enough to permit chemical manipulation yet short enough to minimize destruction of healthy cells due to degradation of the label over time, (3) it can be produced conveniently using a cyclotron, and (4) alpha emission is associated with 100% of its decays with no accompanying beta emission. In the past year the evaluation of an astatine-tellurium colloid as an agent for the destruction of malignant ascites has been completed. The therapeutic efficacy of 211 At-tellurium colloid has been compared with that of several beta-emitting radiocolloids. Studies on the application of monoclonal antibodies as carriers for selective delineation and destruction of malignant cell populations have also been initiated

Development and radiotherapeutic application of /sup 211/At-labeled radiopharmaceuticals. Progress report, March 1, 1981-February 28, 1982

Energy Technology Data Exchange (ETDEWEB)

Adelstein, S.J.; Zalutsky, M.; Bloomer, W.

1981-01-01

This project is concerned with developing the potential of alpha-emitting radionuclides as agents for radiotherapy. Alpha-emitters seem ideally suited for his application because their high linear energy transfer and short range permit the deposition of considerable energy in a very small volume of tissue. Unlike the beta particles of /sup 131/I which have a range of about 1 to 2 mm in tissue, 5 to 7 MeV alpha particles would traverse only a few cell diameters. Among the available alpha-emitters, /sup 211/At appears most promising for therapeutic applications because, (1) it has some chemical similarities to iodine, an element that can readily be incorporated into numerous proteins and peptides, (2) it has a half-life that is long enough to permit chemical manipulation yet short enough to minimize destruction of healthy cells due to degradation of the label over time, (3) it can be produced conveniently using a cyclotron, and (4) alpha emission is associated with 100% of its decays with no accompanying beta emission. In the past year the evaluation of an astatine-tellurium colloid as an agent for the destruction of malignant ascites has been completed. The therapeutic efficacy of /sup 211/At-tellurium colloid has been compared with that of several beta-emitting radiocolloids. Studies on the application of monoclonal antibodies as carriers for selective delineation and destruction of malignant cell populations have also been initiated.

Effect of cetuximab in combination with alpha-radioimmunotherapy in cultured squamous cell carcinomas

International Nuclear Information System (INIS)

Nestor, Marika; Sundstroem, Magnus; Anniko, Matti; Tolmachev, Vladimir

2011-01-01

Aim: The monoclonal antibody cetuximab, targeting the epidermal growth factor receptor (EGFR), is a promising molecular targeting agent to be used in combination with radiation for anticancer therapy. In this study, effects of cetuximab in combination with alpha-emitting radioimmunotherapy (RIT) in a panel of cultured human squamous cell carcinomas (SCCs) were assessed. Methods: SCC cell lines were characterized and treated with cetuximab in combination with anti-CD44v6 RIT using the astatinated chimeric monoclonal antibody U36 ( 211 At-cMAb U36). Effects on 211 At-cMAb U36 uptake, internalization and cell proliferation were then assessed in SCC cells. Results: Cetuximab in combination with 211 At-cMAb U36 mediated increased growth inhibition compared to RIT or cetuximab alone in two cell lines. However, cetuximab also mediated radioprotective effects compared to RIT alone in two cell lines. The radioprotective effects occurred in the cell lines in which cetuximab clearly inhibited cell growth during radiation exposure. Cetuximab treatment also influenced 211 At-cMAb-U36 uptake and internalization, suggesting interactions between CD44v6 and EGFR. Conclusions: Results from this study demonstrate the vast importance of further clarifying the mechanisms of cetuximab and radiation response, and the relationship between EGFR and suitable RIT targets. This is important not only in order to avoid potential radioprotective effects, but also in order to find and utilize potential synergistic effects from these combinations.

Proceedings of transuranium elements

International Nuclear Information System (INIS)

Anon.

1992-01-01

The identification of the first synthetic elements was established by chemical evidence. Conclusive proof of the synthesis of the first artificial element, technetium, was published in 1937 by Perrier and Segre. An essential aspect of their achievement was the prediction of the chemical properties of element 43, which had been missing from the periodic table and which was expected to have properties similar to those of manganese and rhenium. The discovery of other artificial elements, astatine and francium, was facilitated in 1939-1940 by the prediction of their chemical properties. A little more than 50 years ago, in the spring of 1940, Edwin McMillan and Philip Abelson synthesized element 93, neptunium, and confirmed its uniqueness by chemical means. On August 30, 1940, Glenn Seaborg, Arthur Wahl, and the late Joseph Kennedy began their neutron irradiations of uranium nitrate hexahydrate. A few months later they synthesized element 94, later named plutonium, by observing the alpha particles emitted from uranium oxide targets that had been bombarded with deuterons. Shortly thereafter they proved that is was the second transuranium element by establishing its unique oxidation-reduction behavior. The symposium honored the scientists and engineers whose vision and dedication led to the discovery of the transuranium elements and to the understanding of the influence of 5f electrons on their electronic structure and bonding. This volume represents a record of papers presented at the symposium

A Histidine pH sensor regulates activation of the Ras-specific guanine nucleotide exchange factor RasGRP1.

Science.gov (United States)

Vercoulen, Yvonne; Kondo, Yasushi; Iwig, Jeffrey S; Janssen, Axel B; White, Katharine A; Amini, Mojtaba; Barber, Diane L; Kuriyan, John; Roose, Jeroen P

2017-09-27

RasGRPs are guanine nucleotide exchange factors that are specific for Ras or Rap, and are important regulators of cellular signaling. Aberrant expression or mutation of RasGRPs results in disease. An analysis of RasGRP1 SNP variants led to the conclusion that the charge of His 212 in RasGRP1 alters signaling activity and plasma membrane recruitment, indicating that His 212 is a pH sensor that alters the balance between the inactive and active forms of RasGRP1. To understand the structural basis for this effect we compared the structure of autoinhibited RasGRP1, determined previously, to those of active RasGRP4:H-Ras and RasGRP2:Rap1b complexes. The transition from the autoinhibited to the active form of RasGRP1 involves the rearrangement of an inter-domain linker that displaces inhibitory inter-domain interactions. His 212 is located at the fulcrum of these conformational changes, and structural features in its vicinity are consistent with its function as a pH-dependent switch.

ORF Alignment: NC_003888 [GENIUS II[Archive

Lifescience Database Archive (English)

Full Text Available FATMYAAHGVGLAANQVGEAVRVFVYD Sbjct: 1 ... VRPLGLLGDPVLHARCAEVTDFGPELAALVEDLFATMYAAHGVGLAANQVGEAVRVFVYD 60 ... Query: 171 TVRGTGFFARC...LQHECDHLEGRVYADRLTGRRHRKLMRQVAR 212 ... TVRGTGFFARCLQHECDHLEGRVYADRLTGRRHRKLMRQVAR Sbjct: 121 TVRGTGFFARCLQHECDHLEGRVYADRLTGRRHRKLMRQVAR 162

Inelastic excitation of sup 1 sup 8 sup 7 Re

CERN Document Server

Shizuma, T; Oshima, M; Matsuda, M; Hayakawa, T; Koizumi, M; Osa, A; Sugawara, M; Zhang, Y H; Liu, Z

2003-01-01

Excited states in sup 1 sup 8 sup 7 Re populated by inelastic scattering of a 500 MeV sup 8 sup 2 Se beam have been studied by means of in-beam gamma-ray spectroscopy. Levels built on the 5/2 sup + [402] and 9/2 sup - [514] one-quasiparticle states were measured up to I suppi = (21/2 sup +) and (21/2 sup -), respectively. In addition, several new levels including an isomer at 1682 keV with a half-life of 114(23) ns have been found. Quasiparticle configurations of the levels and the transition rates are discussed. (orig.)

Tactical Wheeled Vehicle Fleet Requirements. Volume 3. Appendixes

Science.gov (United States)

1980-10-01

64C, in each case). (3) Annual operating mileage, by payload class ( runners , only). (a) 1/4 T 7200 mi. (b) 5/4 T 6200 mi. (c) 2-1/2 T 3200 mi. (d... runners /nonrunners in Europe. Those figures should still be used. The following figures should be used for CONUS: 0) 1/4 T (2) 5/4 T (3) 2-1/2 T (4...0 a» * 4« M » Ml 00 a* MM «*« «• 0M • * *« «0 » kite MM • • 00 00 aa «0

Spatial and spatio-temporal bayesian models with R - INLA

CERN Document Server

Blangiardo, Marta

2015-01-01

Dedication iiiPreface ix1 Introduction 11.1 Why spatial and spatio-temporal statistics? 11.2 Why do we use Bayesian methods for modelling spatial and spatio-temporal structures? 21.3 Why INLA? 31.4 Datasets 32 Introduction to 212.1 The language 212.2 objects 222.3 Data and session management 342.4 Packages 352.5 Programming in 362.6 Basic statistical analysis with 393 Introduction to Bayesian Methods 533.1 Bayesian Philosophy 533.2 Basic Probability Elements 573.3 Bayes Theorem 623.4 Prior and Posterior Distributions 643.5 Working with the Posterior Distribution 663.6 Choosing the Prior Distr

The biocompatibility of a new endodontic paste used in dental trauma

Directory of Open Access Journals (Sweden)

Adriana de Jesus Soares

Full Text Available AbstractObjectiveThis study investigated the short-term subcutaneous tissue reaction of a new endodontic paste, based on the association among calcium hydroxide [Ca(OH2], 2% chlorhexidine gel (CHX and zinc oxide (ZnO in comparison with a control paste, and the individual substances alone.Material and methodTwenty-four male wistar rats were used. Polyethylene tubes containing the materials were implanted into the dorsal connective tissue of rats. Empty implanted tubes served as controls. The materials used were: Ca(OH2+CHX+ZnO paste in two different proportions (2:1:2 and (2:1:3, a control paste, Ca(OH2+CHX (2:1, and the substances alone. After time intervals of 2, 3, 7 and 15 days, the animals were euthanized and the specimens prepared for histological processing. Data were evaluated qualitatively according to the levels of tissue repair and the degree of inflammation.ResultOn the second day all the materials were observed to cause a severe inflammatory response. On the third day, the pastes Ca(OH2+CHX+ZnO (2:1:2 and (2:1:3 showed reduction in degree of inflammation. On seventh day the tissue was already more organized with presence of collagen fibers in all materials. After 15 days it was found that compound Ca(OH2+CHX+ZnO (2:1:2 showed areas with no inflammatory infiltrate.ConclusionThe experimental pastes, Ca(OH2+CHX+ZnO in two different proportions (2:1:2 and (2:1:3, showed favorable results with respect to biocompatibility, when evaluated for a short-term.

Correlation Water Velocity and TSS with Natural Radionuclides Activity

International Nuclear Information System (INIS)

Tri Harningsih; Muzakky; Agus Taftazani

2007-01-01

Correlation water velocity and TSS with natural radionuclides activity has been studied. For that purpose, the study is to correlation water velocity and TSS with radionuclides on water and sediment samples in alongside river Code Yogyakarta. This research selected radionuclides, for examples Ra-226, Pb-212, Ac- 228, and K-40. Election of this radionuclides to spread over gamma gross composition alongside river of Code. Gamma gross influenced by water velocity and TSS, so that require to correct between water velocity and TSS to radionuclides. Sampling water and sediment conducted when dry season of August, 2006 at 11 locations, start from Boyong Bridge until Pacar Bridge. Result of analysis showed that water velocity range from 8-1070 L/dt and TSS range from 2.81 E-06 - 8.02 E-04 mg/L. The accumulation of radionuclides in water samples non correction water velocity for Ra-226: 0.302-2.861 Bq/L, Pb-212: 0.400-3.390 Bq/L, Ac- 228: 0.0029-0.0047 Bq/L and K-40: 0.780-9.178 Bq/L. The accumulation of radionuclide in water samples correction water velocity for Ra-226: 1.112-70.454 Bq/L, Pb-212: 0.850-77.113 Bq/L, Ac-228: 0.7187- 60.859 Bq/L and K-40: 2.420-208.8 Bq/L. While distribution of radionuclide in sediment for the Ra-226: 0.0012-0.0211 Bq/kg, Pb-212: 0.0017-0.0371 Bq/kg, Ac-228: 0.0021-0.0073 Bq/kg and K-40: 0.0006-0.0084 Bq/kg. (author)

Alpha chain hemoglobins with electrophoretic mobility similar to that of hemoglobin S in a newborn screening program.

Science.gov (United States)

Silva, Marcilene Rezende; Sendin, Shimene Mascarenhas; Araujo, Isabela Couto de Oliveira; Pimentel, Fernanda Silva; Viana, Marcos Borato

2013-01-01

To characterize alpha-chain variant hemoglobins with electric mobility similar to that of hemoglobin S in a newborn screening program. β(S) allele and alpha-thalassemia deletions were investigated in 14 children who had undefined hemoglobin at birth and an electrophoretic profile similar to that of hemoglobin S when they were six months old. Gene sequencing and restriction enzymes (DdeI, BsaJI, NlaIV, Bsu36I and TaqI) were used to identify hemoglobins. Clinical and hematological data were obtained from children who attended scheduled medical visits. THE FOLLOWING ALPHA CHAIN VARIANTS WERE FOUND: seven children with hemoglobin Hasharon [alpha2 47(CE5) Asp>His, HbA2:c.142G>C], all associated with alpha-thalassemia, five with hemoglobin Ottawa [alpha1 15(A13) Gly>Arg, HBA1:c.46G>C], one with hemoglobin St Luke's [alpha1 95(G2) Pro>Arg, HBA1:c.287C>G] and another one with hemoglobin Etobicoke [alpha212 84(F5) Ser>Arg, HBA212:c.255C>G]. Two associations with hemoglobin S were found: one with hemoglobin Ottawa and one with hemoglobin St Luke's. The mutation underlying hemoglobin Etobicoke was located in a hybrid α212 allele in one child. There was no evidence of clinically relevant hemoglobins detected in this study. Apparently these are the first cases of hemoglobin Ottawa, St Luke's, Etobicoke and the α212 gene described in Brazil. The hemoglobins detected in this study may lead to false diagnosis of sickle cell trait or sickle cell disease when only isoelectric focusing is used in neonatal screening. Additional tests are necessary for the correct identification of hemoglobin variants.

A novel atmospheric tritium sampling system

Science.gov (United States)

Qin, Lailai; Xia, Zhenghai; Gu, Shaozhong; Zhang, Dongxun; Bao, Guangliang; Han, Xingbo; Ma, Yuhua; Deng, Ke; Liu, Jiayu; Zhang, Qin; Ma, Zhaowei; Yang, Guo; Liu, Wei; Liu, Guimin

2018-06-01

The health hazard of tritium is related to its chemical form. Sampling different chemical forms of tritium simultaneously becomes significant. Here a novel atmospheric tritium sampling system (TS-212) was developed to collect the tritiated water (HTO), tritiated hydrogen (HT) and tritiated methane (CH3T) simultaneously. It consisted of an air inlet system, three parallel connected sampling channels, a hydrogen supply module, a methane supply module and a remote control system. It worked at air flow rate of 1 L/min to 5 L/min, with temperature of catalyst furnace at 200 °C for HT sampling and 400 °C for CH3T sampling. Conversion rates of both HT and CH3T to HTO were larger than 99%. The collecting efficiency of the two-stage trap sets for HTO was larger than 96% in 12 h working-time without being blocked. Therefore, the collected efficiencies of TS-212 are larger than 95% for tritium with different chemical forms in environment. Besides, the remote control system made sampling more intelligent, reducing the operator's work intensity. Based on the performance parameters described above, the TS-212 can be used to sample atmospheric tritium in different chemical forms.

Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil

Directory of Open Access Journals (Sweden)

Marcela Fernanda Torres Samico Fernandes

2016-03-01

Full Text Available Abstract The aim of the present study was to investigate the occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chickens bred in the metropolitan area of Recife, Brazil. In total, 212 serum samples were collected from 16 properties, and 12 backyard chickens were collected in the six sanitary districts of Recife. An indirect immunofluorescence assay (IFA was used to investigate the occurrence of anti-Toxoplasma gondii antibodies. Polymerase chain reaction (PCR was used to detect T. gondii DNA in brain, heart, liver and lung specimens. Of the samples analyzed by serology, 86/212 (40.56% were positive; of the samples analyzed by PCR, 2/12 (16.7% were positive, with both samples positive by both tests (serological and molecular. The presence of antibody anti-T. gondii and parasite DNA in tissues of these animals are worrying aspects for public health because there is a risk of transmission of the parasite to humans through eating undercooked or raw meat. Based on the results, the adoption of preventive measures to prevent the cats access to the chickens creations should be encouraged, since these animals were identified in most of the studied properties.

ORF Alignment: NC_003361 [GENIUS II[Archive

Lifescience Database Archive (English)

Full Text Available ... [Chlamydophila caviae GPIC] ... Length = 212 ... Query: 1 ... MNKKQRQKTLIAPSIMGGDLACIGAEAKKIEESGADLIHID...VMDGHFVPNLTFGPGVIAA 60 ... MNKKQRQKTLIAPSIMGGDLACIGAEAKKIEESGADLIHIDVMDGHF...VPNLTFGPGVIAA Sbjct: 1 ... MNKKQRQKTLIAPSIMGGDLACIGAEAKKIEESGADLIHIDVMDGHFVPNLTFGPGVIAA 60 ... Query: 121 FSPETSI

Targeted alpha therapy: Applications and current status

International Nuclear Information System (INIS)

Bruchertseifer, Frank

2017-01-01

Full text: The field of targeted alpha therapy has been developed rapidly in the last decade. Besides 223 Ra, 211 At and 212 Pb/ 212 Bi the alpha emitters 225 Ac and 213 Bi are promising therapeutic radionuclides for application in targeted alpha therapy of cancer and infectious diseases. The presentation will give a short overview about the current clinical treatments with alpha emitting radionuclides and will place an emphasis on the most promising clinical testing of peptides and antibodies labelled with 225 Ac and 213 Bi for treatment of metastatic castration-resistant prostate cancer patients with glioma and glioblastoma multiform, PSMA-positive tumor phenotype and bladder carcinoma in situ. (author)

Manufacturing Methods and Technology for Electromagnetic Components. Volume 2. Phase 2

Science.gov (United States)

1980-12-01

elimination of bubbles and voids, The main dibadvantage of using a centrifuge is that the samples placed in the centrifuge must be balanced very...2678 6-18 73D 12 wks. 2304 6-18 73D TABLE 9-13. STORAGE STABILITY OF RTV 627 Time at -65 0 F Visc. (cPs) Worklife (Hrs) Hardness 0 2328 3 69A 1 wk. 2300...2-1/2 69A 5 wks. 2570.5 2-1/2 68A 12 days 10 wks. 2667.5 2-1/2 69A TABLE 9-14. STORAGE STABILITY OF RTV 615 Time at -65 0 F Visc. (cPs) Worklife (Hrs

Nance-Horan syndrome: a contiguous gene syndrome involving deletion of the amelogenin gene? A case report and molecular analysis.

Science.gov (United States)

Franco, E; Hodgson, S; Lench, N; Roberts, G J

1995-03-01

A case of Nance-Horan syndrome in a male is presented, with some features of the condition in his carrier mother and her mother. It is proposed that Nance-Horan syndrome might be a contiguous gene syndrome mapping to chromosome Xp21.2-p22.3. The proband had congenital cataract microphthalmia and dental abnormalities including screwdriver shaped incisors and evidence of enamel pitting hypoplasia. The region Xp21.2-p22.3 also contains the tooth enamel protein gene, amelogenin (AMGX). Using molecular genetic techniques, we have shown that there is no evidence that the AMGX gene is deleted in this case of the Nance-Horan syndrome.

Inhalation exposures at a thorium refinery

International Nuclear Information System (INIS)

Mausner, L.F.

1982-01-01

There is a current interest in the metabolism and health effects of thorium due to its potential use in the 232 Th - 233 U nuclear fuel cycle. The airborne concentrations of thorium, thoron daughters and rare earths in a plant which produced thorium and rare earth chemicals from 1932 to 1973 were calculated from past records of alpha counting and air filter samples. This analysis showed that high airborne concentrations of 232 Th, 220 Rn, 212 Pb, 212 Bi and rare earth elements were sometimes reached during plant operations. Limited measurements on autopsy samples of former employees of the plant showed increased tissue concentrations of thorium and rare earths. (U.K.)

Quantitative And Qualitative Measurement Of Radio- Activity In Sand Samples From Chalet Beach In Songkhla Province

International Nuclear Information System (INIS)

Sukhowattanakit, Jirapa; Kessaratikoon, Prasong; Udomsomporn, Suchin; Thorarit, Wutthidej

2005-10-01

The quantitative and qualitative measurement of radioactivity in 39 sand samples collected from Chalatat beach in Songkhla province are presented. Experimental results were obtained by using a high-purity germanium detector and gamma spectroscopy analysis system and comparing to the standard soil (IAEA SOIL 6) at the Office of Atoms for Peace (OAP). The measuring time of all sand samples is 10,000 seconds. Some radioisotopes such as K-40, Cs-137, Tl-208, Bi-212, Pb-212, Bi-214, Pb- 214, Ra-226 and Ac-228 were found in sand samples. In addition, the radioactivity of Ra-226 and Cs-137 in those samples were found in normal level

Yield and gross income of arracacha in monocrop and intercropping with the Japanese bunching onion and parsley Produção e renda bruta de mandioquinha-salsa em cultivo solteiro e consorciado com cebolinha e salsa

Directory of Open Access Journals (Sweden)

Néstor Antonio Heredia Zárate

2008-06-01

Full Text Available The objective of this work was to study the yield performance and to determine the gross income of arracacha (Arracacia xanthorrhiza Bancroft, cultivar Amarela de Carandaí, grown in monoculture as well as in intercropping with the Japanese bunching onion (Allium fistulosum L., cultivar Todo Ano (A-JBO and parsley (Petroselinum crispum (Mill., cultivar Lisa Preferida (A-P. Arracacha and the Japanese bunching onion were vegetatively propagated, while parsley was propagated by seeds. The Japanese bunching onion was first harvested 87 days after the planting (DAP, with resprouts harvested 154 and 212 DAP. Parsley was first harvested 105 days after the sowing (DAS, and, resprouts, 171, 212, and 268 DAS. Arracacha was harvested 268 DAP. At the harvest carried out 212 DAP, the Japanese bunching onion plants in monocrop significantly exceeded those in intercropping in 1.73 cm height and 0.99 t ha-1 commercial fresh mass (CFM. In parsley, the CFM yield in monocrop significantly exceeded the intercropping figures in 0.63, 0.66, and 0.72 t ha-1 at harvests carried out respectively 171, 212, and 268 DAS. Plant height and CFM of the Japanese bunching onion and parsley increased after every new harvest, except in parsley, for plant height 171 DAS and CFM 268 DAS. No significant differences were found in any of the evaluated characteristics when arracacha as single crop was compared to the A-JBO intercropping arrangement. Nevertheless, the A-P intercropping arrangement significantly reduced all characteristics in relation to the other systems, except for yield of non-commercial arracacha roots. A-JBO intercropping arrangement was viable (LER = 1.49 and therefore may be employed by farmers. A-P intercropping, on its turn, was unfeasible (LER = 0.97.Este trabalho teve como objetivo estudar o desempenho produtivo e determinar a renda bruta obtida a partir do cultivo da mandioquinha-salsa (Arracacia xanthorrhiza Bancroft, cultivar Amarela de Carandaí, em

18 CFR 281.212 - Draft tariff and index of entitlements.

Science.gov (United States)

2010-04-01

... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Draft tariff and index... REGULATORY COMMISSION, DEPARTMENT OF ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES NATURAL GAS CURTAILMENT UNDER THE NATURAL GAS POLICY ACT OF 1978 Permanent Curtailment...

14 CFR 212.5 - Operation of affinity (pro rata) charters.

Science.gov (United States)

2010-01-01

... commercial establishment, industrial plant, or government agency, or (2) Students and employees of a single... organization who: Have not joined the organization merely to travel on a charter flight; and who have been...

212 Emotional Intelligence and Self Efficacy as Correlates of Career ...

African Journals Online (AJOL)

FIRST LADY

2011-01-18

Jan 18, 2011 ... the teaching sector. Without real commitment ..... results of data analysis shows a correlation value of .15 with a computed. F(3,179) = 1.27 P > .05. ... 2007; Coladarchi, 1992; Miner and Woolfok Hoy, 2002). The finding could.

40 CFR 80.212 - What requirements apply to oxygenate blenders?

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2010-07-01

... blend oxygenate into gasoline downstream of the refinery that produced the gasoline or the import facility where the gasoline was imported, are not subject to the requirements of this subpart applicable to... PROGRAMS (CONTINUED) REGULATION OF FUELS AND FUEL ADDITIVES Gasoline Sulfur Gasoline Sulfur Standards § 80...

48 CFR 52.212-1 - Instructions to Offerors-Commercial Items.

Science.gov (United States)

2010-10-01

... Classification System (NAICS) code and small business size standard. The NAICS code and small business size... Government infrastructure not later than 5:00 p.m. one working day prior to the date specified for receipt of...

8 CFR 212.15 - Certificates for foreign health care workers.

Science.gov (United States)

2010-01-01

...) Service International. (iii) International English Language Testing System (IELTS). (4) Passing English... certifying organizations shall not accept the results of the TOEIC, or the IELTS for the occupation of... International: TOEIC: 725; plus TWE: 4.0 and TSE: 50; or (C) IELTS: 6.5 overall with a spoken band score of 7.0...

Metallogenetic characteristics of major fault in area No.212-318

International Nuclear Information System (INIS)

Zhang Yong

1994-01-01

Based on the distribution characteristics of major fault on the plan and assemblage form of major and subsidiary faults on the section, this paper expounds the relationship between major fault and uranium ore-formation, namely: different orders of fault structure control the spatial distribution of ore fields, ore deposits and ore bodies respectively, regional turning sites of the major fault control the ore fields; local turning sites of the major fault control ore deposits; different types of structural assemblages control ore bodies. Whether the matter compositions of fault structure are simple or not, it plays an absolutely important role in ore-formation. The complicated matter compositions of fault structures are favourable for ore-formation, conversely, simple compositions are unfavourable