Mediadores bioquímicos involucrados en la fisiopatología y diagnóstico de la enfermedad periodontal

Directory of Open Access Journals (Sweden)

Facundo Ledesma

2012-09-01

Full Text Available En el desarrollo de la enfermedad periodontal  (EP  intervienen enzimas derivadas tanto del huésped como de los microorganismos presentes en la placa dental.  La presente revisión abordó el papel que cumplen diversos mediadores de la EP y sus utilidades clínicas. La fosfatasa alcalina, la β-glucuronidasa, la catepsina B, las MMP-8 y MMP-9, la elastasa y las Dipeptidil peptidasas II y IV, son útiles en el monitoreo y la planificación del tratamiento. La catepsina B, la MMP-8, la elastasa, las dipeptidil peptidasas II y IV, son utilizadas para diferenciar la gingivitis y la periodontitis. Las exotoxinas bacterianas del Aggregatibacter  actinomycetemcomitans, indican la severidad de la enfermedad periodontal agresiva y ayudan al pronóstico del caso.

Avaliação da fosfatase alcalina óssea e hepática em gatos com tirotoxicose induzida

Directory of Open Access Journals (Sweden)

Fabiano Séllos Costa

2009-10-01

Full Text Available A tirotoxicose é caracterizada pelas excessivas concentrações séricas dos hormônios tiroidianos, podendo desencadear graves alterações no metabolismo ósseo, sendo a elevação da fosfatase alcalina total uma alteração laboratorial freqüentemente observada no hipertiroidismo felino. O aumento global dos níveis séricos de fosfatase alcalina pode ser decorrente de diferentes isoenzimas e, no caso do hipertiroidismo em humanos, as isoenzimas de origem óssea e hepática apresentam-se comumente elevadas. A partir da avaliação da bioquímica sérica de oito gatos com tirotoxicose induzida e elevação da fosfatase alcalina associada, o presente trabalho demonstra um aumento significativamente maior dos níveis séricos da fosfatase alcalina de origem óssea quando comparado com a isoenzima de origem hepática. Conclui-se que as alterações no metabolismo ósseo foram as principais responsáveis pelo aumento da fosfatase alcalina nos gatos com tirotoxicose induzida.

ADIÇÃO DE ANTRAQUINONA NA POLPAÇÃO ALCALINA DE Eucalyptus saligna

OpenAIRE

Lúcia Helena Jerônimo; Celso E. B. Foelkel; Sonia M. B. Frizzo

2000-01-01

O presente trabalho avaliou diferentes variações dos parâmetros de polpações alcalinas de cavacos de madeira de Eucalyptus saligna, tendo como objetivo estudar a influência da antraquinona (AQ), visando a diminuir os compostos reduzidos de enxofre nas emissões aéreas por meio da redução da sulfidez. Foram realizados oito cozimentos com álcali ativo variando de 19 a 21%, sulfidez de 0 a 20% e antraquinona de 0 a 0,1%, visando aos números kappa de 15,5 ± 1,5, em condições alcalinas controladas ...

Acid Fostatase Activity in soils croping with potato and pastures from the Catambuco, Pasto, Colombia Actividad de fosfatasa ácida en suelos cultivados con papa y praderas del corregimiento de Catambuco, Pasto-Colombia

Directory of Open Access Journals (Sweden)

Burbano Hernán

2007-03-01

Full Text Available To estimated the acid phosphatase activity (APA in soil covered with potato and prairie in Catambuco, place Pasto, Colombia; located to 2820 msnm and with average temperature of 12.4oC. The acid phosphatase activity obtained the biggest values in soil covered with prairie in winter time, in addition to that, there were high correlations between the APA and the organic matter off the soil. In the factors sampling times and use of the soil, there were significance statistical differences, with the most significance in soil covered with prairie and with recent use in potato cultivation. The APA obtained the biggest percentage in soil covered with prairie, in front of the soil cultivated with potato for the two evaluated times. Key words: Acid phosphatase activity, use of land, Solanum tuberosumCon el objetivo de estimar y comparar la actividad de la fostatasa ácida (AFA en suelos sembrados con papa y con pastos, se realizó la presente investigación en suelos del corregimiento de Catambuco, municipio de Pasto, Colombia (2.820 msnm, 12.4oC. La mayor actividad de la fosfatasa ácida ocurrió en pradera en época de invierno; además se encontraron altas correlaciones entre AFA y el contenido de materia orgánica. Para los factores épocas de muestreo y uso del suelo hubo diferencias estadísticas altamente significativas y para suelos cubiertos con pradera y con uso reciente en el cultivo de papa. La AFA alcanzó el mayor porcentaje en suelos cubiertos con pradera, frente a los suelos cultivados con papa, para las dos épocas evaluadas. Palabras claves: Actividad fosfatasa ácida, uso del terreno, Solanum tuberosum

Acrodermatitis enteropática: tratamiento con Zinc

OpenAIRE

Arquer Blanc, A. de; Pastor Durán, Xavier; Ajram Maksoud, Jamil; Rodriguez-Hierro, F.; Palomeque, A.

1984-01-01

Se presenta un nuevo caso de Acrodermatitis Enteropática en un lactante de 2,5 meses de vida, fruto de embarazo gemelar bivitelino pretérmino (36 S), de aparición gradual desde los 15 días de vida. Había seguido lactancia artificial exclusivamente desde su nacimiento, al igual que su hermano gemelo que no presentó la enfermedad. Entre los exámenes complementarios destacaba una importante hipozincemia, fosfatasas alcalinas descendidas, alteraciones en la inmunidad celular, rasgos de inmadurez ...

Caracterización bioquímica y funcional de proteínas que interaccionan con fosfatasas 2C en germinación y respuestas a estrés reguladas por ABA.

OpenAIRE

Saavedra Contreras, Xandra Amanda

2013-01-01

[ES] La proteín-fosfatasa FsPP2C1, fue aislada de la especie leñosa Fagus sylvatica (haya), sea descrito como regulador negativo de la señalización del Ácido Abscisico, en esta investigación, para obtener una visión más profunda sobre la función de FsPP2C1, nos proponemos identificar las proteínas que interactúan con esta proteína, mediante ensayo de doble híbrido en levadura, se han identificado dos proteínas (PYL8/RCAR3 y PYL7/RCAR2) miembros de la familia PYR1-PYL/RCAR. Por expres...

Evaluación de la toxicidad hepática y renal aguda y subaguda del látex de Croton palanostigma (sangre de grado en animales de experimentación

Directory of Open Access Journals (Sweden)

Miguel Sandoval

2005-06-01

Full Text Available Objetivo: Determinar la toxicidad hepática y renal de la administración oral del látex de Croton palanostigma, en dosis terapéutica y de mayor concentración. Materiales y Métodos: Se evaluó 60 ratas albinas, machos, en dos grupos: con administración oral aguda por 7 dìas y otro con administración oral subaguda por 30 días ad libitum. En cada grupo se tuvo tres subgrupos: (A Látex diluido 1/1000 (dosis terapéutica, (B Látex diluido 1/100, (C Agua (grupo control. Medimos el peso del animal y consumo del bebedero. Después, bajo anestesia con éter, se obtuvo sangre por punción cardiaca y se centrifugó a 5000 rpm por 5 minutos para obtener plasma sanguíneo. Se determinó actividad de gamma glutamil transferasa y fosfatasa alcalina, se evaluó niveles de urea, creatinina, proteínas totales, albúmina y globulinas por espectrofotocolorimétría. Resultados: El consumo promedio del látex en el primer grupo fue: en A, 0,14 mL.kg-1/día-1/rata-1; en B, 1,34 mL/kg-1/día-1/rata-1; y, en C, agua. En el segundo grupo, fue: en A, 0,13 mL/kg-1/día-1/rata-1; en B, 1,21 mL/kg-1/día-1/rata-1; y, en C, agua. No hubo diferencia significativa en el consumo del látex diario entre los grupos tratados a 7 y 30 días. Las actividades de gamma glutamil transferasa y fosfatasa alcalina, así como los metabolitos sanguíneos y ratios hígado/peso animal y riñones/peso animal, de los grupos experimentales vs. grupo control, no presentaron diferencias significativas (p>0,05. Conclusiones: En condiciones experimentales, la administración oral de sangre de grado, aguda y subaguda, en dosis terapéuticas y de mayor concentración, no produce alteraciones bioquímicas hepáticas ni renales en animales de experimentación.

ESTABILIZACIÓN ALCALINA DE BIOSÓLIDOS COMPOSTADOS DE PLANTAS DE TRATAMIENTO DE AGUAS RESIDUALES DOMÉSTICAS PARA APROVECHAMIENTO AGRÍCOLA ALKALI STABILIZATION OF COMPOSTED BIOSOLIDS FROM DOMESTIC WASTEWATER TREATMEN PLANTS FOR AGRICULTURE PURPOSE

Directory of Open Access Journals (Sweden)

Patricia Torres Lozada

2008-06-01

Full Text Available Uno de los limitantes del aprovechamiento agrícola de lodos y biosólidos producidos por plantas de tratamiento de aguas residuales domésticas - PTAR es su calidad microbiológica y parasitológica. Se evaluó la estabilización alcalina del compost obtenido a partir la planta de tratamiento de aguas residuales Cañaveralejo de Cali, Colombia (PTAR-C, utilizando ceniza de calderas de una industria papelera, Cal Hidratada (CH y Cal Viva (CV, en combinaciones con el compost del 8, 15 y 30% para CH y ceniza y de 15% para CV, en proporciones peso a peso. Durante 13 días se monitoreó temperatura, pH, humedad, coliformes fecales y huevos de helmintos. Los resultados obtenidos mostraron que CV y CH al 15% lograron elevar el pH a 12 unidades por más de 72 horas y obtener cero huevos de helmintos viables, lo que muestra una eficiente reducción de patógenos y el alcance de estándares para compost clase A, lo que no se alcanzó con la ceniza en las proporciones evaluadas. En términos de humedad, CV al 15% presentó mejor desempeño que CH, la cual requirió un 30% y de 3 a 5 días para reducir la humedad hasta el 20% sugerido para la aplicación agrícola de compost. Es recomendable evaluar rangos entre 8 y 15% de CV y CH, otras cenizas alcalinas y mezclas para reducir tiempos de tratamiento, requerimientos de área y costos, además considerar la remoción de otros indicadores en plantas y humanos como fitopatógenos y Salmonella.The limitation for agriculture use of sludge and biosolids from wastewater treatment plants is the microbiological and parasitological quality. Alkali stabilization was evaluated in produced compost from biosolids of Cañaveralejo wastewater treatment plant (PTAR-C, based in Cali, Colombia . Ashes, quick and slake lime were applied to compost. Doses of 8, 15 and 30% of ash and hydrated lime and 15% of quick lime were the concentration (in weight used; during 13th days TºC, pH, humidity, faecal coliforms and helmints

Residuos cerámicos para su posible uso como materia prima en la fabricación de clínker de cemento Portland: caracterización y activación alcalina

Directory of Open Access Journals (Sweden)

Martínez-Ramírez, S.

2006-03-01

Kioto. El empleo de diferentes residuos y subproductos industriales como materiales alternativos en la fabricación de cemento se ha revelado como un medio muy efectivo para alcanzar, en gran parte, esa sostenibilidad. Estos materiales alternativos pueden ser incorporados en el proceso de fabricación de cemento, por la sustitución parcial de los combustibles convencionales y/o de las materias primas del crudo y por la incorporación de adiciones activas al cemento y/o clínker.En el presente trabajo se exponen los resultados preliminares obtenidos al estudiar la composición y activación alcalina de seis residuos cerámicos cocidos con vistas a su viabilidad de sustituir, parcialmente, algunas materias primas, utilizadas en la preparación de un crudo de cemento.Los resultados obtenidos han mostrado que los seis residuos tienen una composición química y mineralógica adecuada para su posible empleo como materia prima alternativa de un crudo de cemento Portland. No son ni tóxicos ni radioactivos. Como era de esperar estos residuos tienen capacidad puzolánica. A la edad de estudio (8 días la activación alcalina de los residuos es baja. El contenido en fase amorfa y la naturaleza del activador no parecen ser factores que influyan en dicho proceso de activación. El estudio de caracterización realizado sobre las pastas activadas ha demostrado que las fases feldespáticas son las más susceptibles de disolución y reacción con las disoluciones alcalinas.

Estudo da qualidade de manteigas por fosfatase alcalina e análises físico-químicas

Directory of Open Access Journals (Sweden)

Cristina Andrade da Silva

2009-08-01

Full Text Available Foram avaliadas as características físico-químicas e atividade da fosfatase alcalina em manteigas comercializadas em Viçosa-MG. As amostras foram coletadas e avaliadas entre agosto e novembro de 2007. Foram utilizadas amostras indicativas de três lotes de dez marcas de manteiga em um total de trinta amostras, submetidas à análise de fosfatase alcalina pelo método modificado de Sharer e as análises físico-químicas foram realizadas de acordo com a Instrução Normativa 68 de 12/2006 do Ministério de Agricultura Pecuária e Abastecimento (MAPA. As amostras apresentaram atividade de fosfatase alcalina em 50% das marcas em pelo menos um lote; 46,66% das amostras indicativas de lote apresentaram umidade fora do padrão; 37% das amostras analisadas apresentaram matéria gorda fora do padrão; 73.33% das amostras apresentaram extrato seco desengordurado acima do limite máximo estabelecido; 13,33% das amostras apresentaram valores superiores de acidez; 50% das amostras apresentaram índice de peróxido fora do padrão e apenas uma amostra indicativa de um lote de uma marca apresentou percentual de NaCl acima do padrão estabelecido.

Avaliação em laboratório do uso de sistemas de coberturas alcalinas para prevenção da drenagem ácida de mina

Directory of Open Access Journals (Sweden)

Adriana Trópia de Abreu

2012-06-01

Full Text Available A drenagem ácida de mina (DAM é um problema ambiental em escala mundial. Ela é proveniente do processo de oxidação química de materiais da mineração que contenham minerais sulfetados expostos a condições atmosféricas com a mediação de bactérias. As águas ácidas geradas nesses ambientes podem comprometer a qualidade dos recursos hídricos. Para tentar conter essa DAM, métodos de prevenção e remediação têm sido aplicados. As coberturas secas têm sido estudadas como alternativa de prevenção, visto que a remediação tem mostrado-se de custo elevado. O objetivo deste trabalho foi avaliar o uso de coberturas alcalinas para controlar a geração de ácido proveniente do estéril da mineração de urânio, U, por meio de sistemas colunas de lixiviação. Para isso, foram estudados sistemas de coberturas que têm como agentes de neutralização a lama vermelha, o calcário e a cal. Amostras de estéril e de água foram coletadas na mina Osamu Utsumi, em Caldas, MG. Foram realizadas análises químicas e mineralógicas do estéril, da cal, do calcário e da lama vermelha. Para os testes cinéticos, foram montadas sete colunas de lixiviação: C1(estéril; C2 (estéril + cobertura de lama vermelha; C3 (estéril + cobertura de calcário; C4 (estéril + cobertura de cal; COB2 (cobertura de lama vermelha; COB3 (cobertura de calcário; e COB4 (cobertura de cal. As amostras lixiviadas foram monitoradas por 100 dias. Os resultados mostraram que a cal e a lama vermelha têm maior potencial neutralizador. No entanto, esses agentes neutralizantes oferecem o inconveniente de introduzir material solúvel no lixiviado. Dessa forma, considerando os parâmetros avaliados, foi demonstrado que o uso de mistura de lama vermelha com estéril do Bota-fora 4 (BF4 pode ser uma alternativa viável para o controle de geração de DAM associada à reciclagem de resíduos industriais alcalinos.

APLICACIÓN DE LA METODOLOGÍA DE SUPERFICIE DE RESPUESTA EN UN PROCESO DE ABSORCIÓN DEL CO2 DE UN BIOGÁS EN UNA SOLUCIÓN ALCALINA

OpenAIRE

MUÑOZ SIERRA, JULIÁN DAVID; CAMARGO TRILLOS, DIEGO ALEJANDRO; GALLEGO SUÁREZ, DARÍO

2009-01-01

Se empleó un diseño factorial 2³ ampliado a central compuesto para investigar los efectos de las variables relación [CO3=/HCO3−], presión inicial del gas y temperatura del sistema gas-líquido, en el proceso de absorción de CO2 en una solución alcalina de carbonato-bicarbonato de sodio, utilizando un biogás simulado como mezcla gaseosa problema. Se encontró una significante influencia de la relación de concentración en el proceso de remoción del dióxido de carbono, obteniéndose un modelo de su...

Eliminación de patógenos en biosólidos por estabilización alcalina Eliminating pathogens in biosolids by alkaline stabilization

Directory of Open Access Journals (Sweden)

Patricia Torres Lozada

2009-07-01

Full Text Available La Planta de Tratamiento de Aguas Residuales de Cañaveralejo -PTAR-C de Cali- Colombia, produce alrededor de 100 t/día de biosólidos que, aunque no tienen restricción por metales pesados, son clase B por el nivel de microorganismos patógenos y parásitos. En un diseño completamente al azar, conformado por seis tratamientos con su respectivo duplicado, se evaluó la estabilización alcalina con dosis del 9% peso a peso de cal viva e hidratada, aplicada a pilas de 0.5 t de biosólidos húmedos (66.5% y secos a temperatura ambiente (25 - 31°C durante 72 h (humedad 50.1%. Con la estabilización alcalina el pH aumentó a valores superiores a 12 unidades durante el tiempo suficiente para garantizar la reducción de patógenos y parásitos, alcanzando un material clase A; sin embargo, el biosólido seco facilitó la formación de grumos que dificultaron las labores de homogenización del sustrato con los alcalinizantes, factor indeseable para la eficiente reducción de patógenos.The Cañaveralejo wastewater treatment plant (PTAR-C based in Cali-Colombia, produces almost 100 t-day-1 of biosolids. Although do not have heavy metals restrictions, it is class B for high contents of pathogens microorganisms and parasites. The alkali stabilization was done with a 9% of dose (w/w of quicklime and hydrated lime applied to different 0.5 ton piles of wet biosolids (66.5% humidity and dry biosolids an environmental temperature (25-31°C for 72 hours (50.1% humidity. The experiment had a completely randomized design and it was composed by 6 treatments with their respective duplicated. With the alkali stabilization, the pH increments above 12 units during enough time to assure pathogens and parasites reduction in order to achieve a class A material level. On the other hand, the dry biosolids facilitate the conditions for lumps formation that reduce the homogenization of the substrate with the alkali material, which it is and undesirable factor for pathogen

Correlação entre a densidade mineral óssea e níveis séricos de cálcio, fósforo e fosfatase alcalina total em gatos após tirotoxicose experimental

Directory of Open Access Journals (Sweden)

Fabiano Séllos Costa

2009-04-01

Full Text Available O hipertiroidismo é caracterizado por concentrações séricas excessivas dos hormônios tiroidianos, podendo causar efeitos sobre o tecido ósseo em humanos e animais. Para melhor caracterização dos efeitos da tirotoxicose no metabolismo ósseo, 16 gatos foram induzidos ao estado hipertiroideo. Os resultados demonstraram que, em alguns momentos experimentais, uma maior desmineralização óssea da extremidade distal do rádio promoveu elevação dos níveis séricos de fósforo e fosfatase alcalina total.

Clasificación clínica y comportamiento de las poblaciones linfocitarias en VIH positivos

Directory of Open Access Journals (Sweden)

R. H. Corral

1994-09-01

Full Text Available Con el fin de determinar el comportamiento de las diferente poblaciones linfocitarias, a través de los diferentes estadios clínicos de la infección por e lvirus de inmunodeficiencia humana (VIH establecidos por el CDC, se realizaron tipificaciones de linfocitos T, B, T CD4 y T CD8 en 155 individuos VIH positivos del ISS en Cali, utilizando la técnica de inmunocitoquimica de los complejos fosfatasa alcalina-antifosfatasa alcalina (APAAP en extendidos de sangre periférica. En los pacientes del estadio II, los linfocitos T, B y CD4 se encontraron dentro de los valores normales para la población general, mientras que los CD8 estaban aumentados. En el estadio III, el comportamiento de las poblaciones de linfocitos fue similar salvo un ligero aumento de los linfocitos B. Estos estadios sólo se diferenciaron clínicamente por la presencia de adenopatias. En el estadio IV se observó una marcada depleción detodas las poblaciones linfocitarias estudiadas. En virtud al aumento temprano de CD8 en los estadios II y III, la relación CD41CD8 presenta alteraciones y es menor de 1 ,O aunque el recuento de CD4 en estos estadios sea normal. Sin embargo, cuando ocurre la depleción general en el estadio IV, su utilidad es limitada y el recuento absoluto de CD4 es el indicador más útil en la progresión de la enfermedad. En virtud a que el recuento de linfocitos CD4 y CD8 constituye un pilar y en él se orientan la mayoría de las conductas profilácticas y de tratamiento que se adoptan en los VIH positivos, se hace necesario conducir los esfuerzos para hacer más asequible esta metodología a los pacientes, así como plantear la implementación de otras pruebas evaluadoras de la inmunidad, útiles como indicadores de la progresión de la enfermedad.

Aplicación de la metodología de superficie de respuesta en un proceso de absorción del co2 de un biogás en una solución alcalina

OpenAIRE

MUÑOZ SIERRA, JULIÁN DAVID; CAMARGO TRILLOS, DIEGO ALEJANDRO; GALLEGO SUÁREZ, DARÍO

2010-01-01

Se empleó un diseño factorial 23 ampliado a central compuesto para investigar los efectos de las variables relación [CO3=/HCO3−], presión inicial del gas y temperatura del sistema gas-líquido, en el proceso de absorción de CO2 en una solución alcalina de carbonato-bicarbonato de sodio, utilizando un biogás simulado como mezcla gaseosa problema. Se encontró una significante influencia de la relación de concentración en el proceso de remoción del dióxido de carbono, obteniéndose un modelo de su...

Anti-inflammatory and toxicity studies of atranorin extracted from Cladina kalbii Ahti in rodents

Directory of Open Access Journals (Sweden)

Marcelia Garcez Dória de Melo

2011-12-01

Full Text Available Atranorin (ATR is the main compound from the lichen Cladina kalbii Ahti, which grows in the arid regions of northeastern Brazil. This study was conducted to evaluate the anti-inflammatory and toxicological properties of ATR. To evaluate anti-inflammatory properties, paw edema was induced by injecting 0.1 mL of carrageenan into the subplantar region of the right hind paw of rats, and leukocyte migration was induced by injection of 500 µL of carrageenan into the peritoneal cavity of mice. In addition, we determined ATR cytotoxicity in L929 cells by MTT assay and acute (5 g/kg-single dose and subchronic (50 mg/kg-30 days toxicity tests in Wistar rats. The results showed that ATR (100 mg/kg and 200 mg/kg exhibited significant anti-inflammatory activity (paw edema and leukocyte migration. In the acute toxicity test, the animals showed hypoactivity and lethargy during the initial period (first 6 hours and increase in total protein, total and indirect bilirubin, and alkaline phosphatase after 14 days in ATR-treated male rats. The subchronic toxicity test revealed increases in total protein, globulin, gamma-glutamyl transferase, alkaline phosphatase, and total and direct bilirubin in ATR-treated female rats. Histological analysis revealed no changes in the architecture and morphology of the organs. These results suggest that ATR has significant anti-inflammatory activity, with no significant acute and subchronic toxicity or cytotoxicity.Atranorina (ATR é o principal composto do líquen Cladina kalbii Ahti, que cresce em terras áridas do nordeste brasileiro. Este estudo foi realizado para avaliar as propriedades antiinflamatórias e toxicológicas da ATR. Para avaliar as propriedades antiinflamatórias, o edema de pata foi induzido, administrando-se 0,1 mL de carragenina na região subplantar da pata traseira direita e a migração leucocitária foi induzida pela injeção de 500 µL de carragenina no peritônio. Além disso, determinou-se a

APLICACIÓN DE LA METODOLOGÍA DE SUPERFICIE DE RESPUESTA EN UN PROCESO DE ABSORCIÓN DEL CO2 DE UN BIOGÁS EN UNA SOLUCIÓN ALCALINA

Directory of Open Access Journals (Sweden)

JULIÁN DAVID MUÑOZ SIERRA

2009-01-01

Full Text Available Se empleó un diseño factorial 23 ampliado a central compuesto para investigar los efectos de las variables relación [CO3=/HCO3], presión inicial del gas y temperatura del sistema gas-líquido, en el proceso de absorción de CO2 en una solución alcalina de carbonato-bicarbonato de sodio, utilizando un biogás simulado como mezcla gaseosa problema. Se encontró una significante influencia de la relación de concentración en el proceso de remoción del dióxido de carbono, obteniéndose un modelo de superficie de respuesta de segundo orden validado estadísticamente mediante análisis de varianza, el cual explica cerca del 98.41% de la variabilidad del cambio del porcentaje (v/v de CO2 en el biogás simulado. Se logró una máxima remoción de CO2 del 62.86% para un tiempo de contacto de sesenta minutos.

Membranas biológicas homólogas preservadas em solução alcalina seguida de liofilização, glicerina a 98% e por liofilização para implantação em eqüinos Homologous biological membranes persevered in alkaline solution and lyophilized, glycerin 98% and by lyophilization for equines implantation

Directory of Open Access Journals (Sweden)

Valcinir Aloisio Scolla Vulcani

2008-08-01

Full Text Available Objetivou-se, neste trabalho, avaliar as alterações físico-químicas proporcionadas pelo tratamento de centros tendinosos diafragmáticos homólogos em solução alcalina seguida de liofilização, para implantação na fáscia interna do músculo reto do abdome de eqüinos. As amostras foram tratadas em períodos de 24, 48, 72, 120 e 144 horas, liofilizadas e analisadas quanto à homogeneidade, flexibilidade e resistência à sutura das amostras. Posteriormente foram caracterizadas por calorimetria exploratória diferencial e microscopia eletrônica de varredura. Para a implantação nos eqüinos, foram utilizadas amostras tratadas por 72 horas seguidas de liofilização, amostras conservadas em glicerina 98% e amostras apenas liofilizadas, que foram retiradas após uma, nove e 18 semanas para avaliar a existência de aderências. Verificou-se que a homogeneidade e a flexibilidade são diretamente proporcionais ao aumento do tempo de tratamento em solução alcalina, enquanto que a resistência é inversamente proporcional ao aumento de tempo, sendo o tratamento por 72 horas intermediário para estas características. A calorimetria exploratória diferencial mostrou que o tratamento não desnatura o colágeno presente nas amostras. Na microscopia eletrônica de varredura, observou-se que o aumento de tempo de tratamento proporciona expansão de zonas menos densas do material. Em relação à formação de aderências, as amostras apenas liofilizadas apresentaram grau máximo na formação da classificação proposta, seguida pelas amostras conservadas em glicerina 98% com grau médio e as amostras tratadas em solução alcalina e liofilizadas, que foram classificadas em grau mínimo. Concluiu-se que o tratamento por 72 horas seria mais apropriado para implantação e que a integração tissular com a parede abdominal foi melhor em relação às amostras apenas liofilizadas e às conservadas em glicerina.The objectives in this research were

EFECTO DE LA ADICIÓN DE CEPAS PROBIÓTICAS SOBRE METABOLITOS SANGUÍNEOS EN CERDOS EN CRECIMIENTO

Directory of Open Access Journals (Sweden)

SANTIAGO LONDOÑO-PÉREZ

Full Text Available El destete es la fase más crítica del cerdo, produciendo trastornos intestinales y diarreas. Para prevenirlo, se han utilizado antibióticos promotores de crecimiento (APC, generando residuos medicamentosos en el producto final. Como alternativa, se utilizan bacterias probióticas que favorecen la síntesis proteica y el metabolismo lipídico. El objetivo fue identificar cambios en metabolitos plasmáticos en cerdos posdestete que consumieron diferentes cepas probióticas. 80 lechones (destetados a 21 días consumieron dos dietas: dieta comercial con y sin la adición de antibiótico (con adición de probióticos L. casei, L. acidophilus o E. faecium en el agua. Se tomaron muestras sanguíneas (días 15, 30 y 45 posdestete para determinar cambios plasmáticos de metabolitos. Se observó un aumento (P<0,05 en niveles plasmáticos de Calcio, Fósforo, glucosa y Fosfatasa alcalina; además, disminuyeron los niveles de Creatinina, triglicéridos y ALT (alanino-transferasa en animales que consumieron E. faecium, comparándolos con los que consumieron antibiótico. Los probióticos (especialmente E. faecium, pueden ser considerados como una alternativa al uso de APC en dietas de cerdos, pues mejoran el estado de órganos digestivos y actúan como promotores naturales del crecimiento ya que mejoran el estado metabólico del animal con cambios positivos en los niveles sanguíneos de Calcio, Fósforo y Glucosa.

Inmunofenotipaje y supervivencia global de pacientes pediátricos con leucemias agudas Immunophenotyping and global survival of pediatric patients with acute leukemia

Directory of Open Access Journals (Sweden)

Vianed Marsán Suárez

2002-04-01

Full Text Available El inmunofenotipaje celular (IFC permite identificar la línea específica de origen de las células leucémicas y su nivel de maduración. Se determinó la frecuencia de los distintos subtipos inmunológicos de leucemias agudas (LA y su posible relación con la supervivencia global de los pacientes. Se estudiaron 117 niños con LA entre 1983 y 1999. El IFC se realizó mediante el ultramicrométodo inmunocitoquímico y el de fosfatasa alcalina-antifosfatasa alcalina. Del total de LA, 77(65,8 % fueron linfoides agudas (LLA, 26 (22,2 % mieloides agudas, 9 (8 % LA indiferenciadas y 5 (4 % se clasificaron como LA híbridas. Del total de LLA, 59 (76,6 % fueron de fenotipo B y 18 (23,4 % de fenotipo T. Se observó una mayor sobrevida en los pacientes de linaje B en relación con los de linaje T y mieloide, con una diferencia muy significativa (p Cell immunophenotyping (CIP identifies the specific line of origin of leukemic cells and their maturing level. The frequency of the various immunological subtypes of acute leukemias (AL and their possible relationship with global survival rate of patients were determined. One hundred and seventeen children with AL were studied from 1983 to 1999. The CIP was conducted through the immunocytochemical ultramicromethod and the alkaline phosphatase - anti-alkaline phosphatase method. Seventy seven (65,8% of all AL were acute lymphoid leukemias (ALL, 26(22,2% acute myeloid (AML, 9 (8% undifferentiated AL, and 5 (4% were hybrid AL. Fifty-nine (76,6% ALL belonged to phenotype B and 18 (23,4% to phenotype T. A higher survival rate was observed in phenotype B ALL patients than in phenotype T ALL and myeloid anemia patients, with a very significant difference (p< 0,001. Survival was also analyzed among the various types of phenotype B acute leukemias; a lower survival rate was observed in patients with more immature phenotypes

Isolamento e caracterização de fibroblastos gengivais positivos para fosfatase alcalina de pacientes portadores de periodontite crônica e hiperplasia gengival medicamentosa = Isolation and characterization of gingival fibroblasts positive for alkaline phosphatase in patients with chronic periodontitis and drug-induced gingival hyperplasia

Directory of Open Access Journals (Sweden)

Santos, Vagner Rodrigues

2010-01-01

Full Text Available Objetivo: Investigar a expressão da atividade de fosfatase alcalina (ALP em fibroblastos gengivais de sujeitos com periodontite crônica (PC e hiperplasia gengival medicamentosa (HGM induzida por difenilhidantoína. Metodologia: Os fragmentos gengivais foram obtidos de 13 pacientes (8 mulheres e 5 homens, 22 a 74 anos, sendo 4 fragmentos procedentes de gengiva clinicamente normal (GN, 5 de biópsias de bolsas periodontais com PC e 4 de HGM induzida pela difenilhidantoína. Através de digestão enzimática, suspensões de células gengivais foram preparadas contendo fibroblastos ALP-positivos sem afetar a atividade da enzima ALP. Análises citoquímica e histoquímica foram realizadas. Resultados: Fibroblastos de GN apresentaram fraca positividade para ALP quando comparados com PC e HGM. PC e HGM exibiram níveis elevados e intermediários, respectivamente, de ALP. Observou-se menor proliferação celular na cultura de células em PC e HGM que em GN. Entretanto, a quantidade de células recuperadas das sub-culturas foi similar à quantidade recuperada na cultura inicial para as três linhagens. Conclusões: A expressão de ALP na PC e na HGM encontra-se aumentada e ocorre baixa proliferação de fibroblastos na PC e na HGM. Isto sugere que a inflamação periodontal e a difenilhidantoína podem influenciar a expressão de ALP e a multiplicação de fibroblastos gengivais humanos. Outros estudos são necessários para melhor avaliar a importância de ALP no desenvolvimento e na progressão de PC e HGM

Efecto de una sola dosis de ácido zoledrónico en un caso de enfermedad de Paget ósea

Directory of Open Access Journals (Sweden)

Melina Sabán

2010-10-01

Full Text Available La enfermedad de Paget es un trastorno crónico del remodelado óseo, caracterizado por un aumento de la resorción ósea producido por osteoclastos atípicos, seguido por un incremento acelerado de la formación ósea, lo que resulta en la formación de hueso en mosaico desorganizado. Un excelente marcador bioquímico para orientar el diagnóstico y seguimiento es la fosfatasa alcalina (FAL. Se presenta el caso de un paciente de 90 años, de sexo masculino, con diagnóstico de enfermedad de Paget. Se inicia tratamiento con pamidronato vía oral con respuesta parcial, por lo que se rota a pamidronato endovenoso. Disminuyen el dolor y la concentración plasmática de FAL, persistiendo con centellograma óseo patológico. Luego de varios años de tratamiento, con adecuado aporte de calcio y vitamina D, comienza nuevamente con dolor y valores elevados de FAL. Se decide iniciar tratamiento con ácido zoledrónico endovenoso 4 mg, única aplicación, obteniéndose remisión clínica y bioquímica desde hace cuatro años y mejoría de la imagen centellográfica. Este informe refiere la buena respuesta, sostenida en el tiempo, al tratamiento con única dosis de ácido zoledrónico en un paciente que presentó resistencia al pamidronato.

Para-Hermitian and para-quaternionic manifolds

International Nuclear Information System (INIS)

Ivanov, S.; Zamkovoy, S.

2003-10-01

A set of canonical para-Hermitian connections on an almost para-Hermitian manifold is defined. A Para-hermitian version of the Apostolov-Gauduchon generalization of the Goldberg-Sachs theorem in General Relativity is given. It is proved that the Nijenhuis tensor of a Nearly para-Kaehler manifolds is parallel with respect to the canonical connection. Salamon's twistor construction on quaternionic manifold is adapted to the para-quaternionic case. A locally conformally hyper-para-Kaehler (hypersymplectic) flat structure with parallel Lee form on the Kodaira-Thurston complex surfaces modeled on S 1 x SL (2, R)-tilde is constructed. Anti-self-dual locally conformally hyper-para-Kaehler (hypersymplectic) neutral metrics with non vanishing Weyl tensor are obtained on the Inoe surfaces. An example of anti-self-dual neutral metric which is not locally conformally hyper-para-Kaehler (hypersymplectic) is constructed. (author)

Para-Hermitian and para-quaternionic manifolds

Energy Technology Data Exchange (ETDEWEB)

Ivanov, S [University of Sofia ' St. Kl. Ohridski' , Faculty of Mathematics and Informatics, Sofia (Bulgaria) and Abdus Salam International Centre for Theoretical Physics, Trieste (Italy); Zamkovoy, S [University of Sofia ' St. Kl. Ohridski' , Faculty of Mathematics and Informatics, Sofia (Bulgaria)

2003-10-01

A set of canonical para-Hermitian connections on an almost para-Hermitian manifold is defined. A Para-hermitian version of the Apostolov-Gauduchon generalization of the Goldberg-Sachs theorem in General Relativity is given. It is proved that the Nijenhuis tensor of a Nearly para-Kaehler manifolds is parallel with respect to the canonical connection. Salamon's twistor construction on quaternionic manifold is adapted to the para-quaternionic case. A locally conformally hyper-para-Kaehler (hypersymplectic) flat structure with parallel Lee form on the Kodaira-Thurston complex surfaces modeled on S{sup 1} x SL (2, R)-tilde is constructed. Anti-self-dual locally conformally hyper-para-Kaehler (hypersymplectic) neutral metrics with non vanishing Weyl tensor are obtained on the Inoe surfaces. An example of anti-self-dual neutral metric which is not locally conformally hyper-para-Kaehler (hypersymplectic) is constructed. (author)

Evaluación de grupos funcionales (ciclo del C, N, P y actividad de la fosfatasa ácida en dos suelos agrícolas del departamento de Boyacá (Colombia

Directory of Open Access Journals (Sweden)

Torres Martha Viviana

2006-12-01

Full Text Available

La comunidad microbiana de los suelos agrícolas es un componente con múltiples funciones esenciales en la fertilidad del suelo. En este trabajo se hace un estudio de las poblaciones de bacterias implicadas en los ciclos biogeoquímicos del C, N y P, con el fin de evaluar su presencia en cultivos de cebolla (Allium ampeloprasum y papa (Solanum tuberosum, así como también, de determinar posibles interacciones entre estos  grupos, tanto en la rizosfera como el suelo total. Además, se realizó la determinación de la actividad enzimática de la fosfatasa ácida. La evaluación de los grupos funcionales se realizó mediante recuento en placa y Características dos doadores de córneas do Banco de Olhos de Cascavel: impacto do exame anti-HBc para hepatite B

Directory of Open Access Journals (Sweden)

Cesar Nobuo Shiratori

2011-02-01

Full Text Available OBJETIVOS: Traçar o perfil dos doadores de tecidos oculares humanos na região de atuação do Banco de Olhos de Cascavel - Paraná (PR e verificar o impacto da positividade da sorologia positiva para hepatite B (anti-HBc no descartes dos tecidos oculares. MÉTODOS: Estudo do tipo transversal de dados do Banco de Olhos de Cascavel do período de março de 2006 a abril de 2007. Informações como idade, sexo, causa de óbito, tempo entre óbito e enucleação e resultados sorológicos de doadores foram coletadas. Dados foram agrupados para caracterização da população de estudo e distribuídos segundo positividade da sorologia para hepatite B (anti-HBc. RESULTADOS: A média de idade dos doadores foi de 54,2 ± 20,6 anos e a maioria era do sexo masculino (64,7%. Tempo médio entre óbito e enucleação foi de 3,8 ± 2,4 horas, a principal causa de óbito foram doenças cardiovasculares (34,5%. Positividade para os marcadores anti-HBc e HBsAg foi de 47,4% e 1,5%, respectivamente, sendo os exames sorológicos para hepatite B a maior causa de descarte dos tecidos oculares doados. Doadores com sorologia positiva para anti-HBc apresentaram maior média de idade comparados com doadores com sorologia negativa (p<0,001. Somente 0,8% das doações apresentaram sorologia positiva para hepatite C. Não houve doações com sorologia para HIV positiva no período do estudo. CONCLUSÕES: Implementação do Banco de Olhos de Cascavel aumentou significativamente a disponibilização de córneas para transplante no Estado do Paraná. A principal contraindicação para disponibilização de tecidos oculares foi a positividade na sorologia para anti-HBc. Estudos de biosegurança devem ser realizados a fim de investigar o potencial de transmissão da hepatite B de doadores positivos para esse marcador. A inclusão desses doadores como doações efetivas acarretaria maior disponibilização de tecidos para transplantes.

A realização do teste anti-hiv no pré-natal: os significados para a gestante El establecimiento de la lucha contra la prueba del vih en pre-navidad: significados para el embarazo The establishment of anti-hiv test in pre-natal: meanings for pregnancy

Directory of Open Access Journals (Sweden)

Roberta Maria de Oliveira Silva

2008-12-01

Full Text Available O estudo teve por objetivo conhecer e analisar o significado da realização do teste anti-HIV no pré-natal para as gestantes. Trata-se de uma pesquisa com abordagem qualitativa e foi realizada em um Hospital Escola e em uma Maternidade do município do Rio de Janeiro. Como recurso técnico-metodológico utilizou-se o discurso do sujeito coletivo (DSC. Após a análise dos discursos verificamos que para as gestantes a realização do teste significa a possibilidade de prevenir a transmissão vertical do HIV e como parte da assistência pré-natal. O pré-natal foi considerado pelas gestantes uma excelente oportunidade para a realização do teste anti HIV, para o conhecimento da condição sorológica e início precoce do tratamento. Conclui-se que o teste, para a maioria das gestantes, representa a possibilidade de proteger o filho do HIV, além de fazer parte da construção do papel materno a partir de um cuidado concreto com a saúde do bebê.El estudio que tenía para que el objetivo sepa y analice el significado de la realización del anti-VIH de la prueba en el prenatal para las mujeres embarazadas. Uno está sobre una investigación con acercamiento cualitativo y fue ejecutado en una escuela del hospital y una maternidad de la ciudad de Rio de Janeiro. Como recurso técnico-metodológico el discurso del ciudadano colectivo fue utilizado (DSC. Después de que el análisis de los discursos, nosotros verificamos que para las mujeres embarazadas la realización de la prueba significa la posibilidad para prevenir la transmisión vertical del VIH y como parte de la ayuda prenatal. El prenatal era considerado por las mujeres embarazadas una ocasión excelente para la realización del anti VIH de la prueba, para el conocimiento de la condición sorological y del principio precoz del tratamiento. Se concluye que la prueba, para la mayoría de las mujeres embarazadas, representa la posibilidad para proteger al hijo del VIH, más allá de ser

PROCESO ENZIMATICO PARA LA PRODUCCION DE METIL ESTERES DE ACIDOS GRASOS UTILIZANDO ACEITES RESIDUALES DE FRITURA EN MEZCLA CON ACEITE DE RAPS COMO MATERIA PRIMA

OpenAIRE

AZOCAR ULLOA; LAURA HAYDEE; AZOCAR ULLOA; LAURA HAYDEE

2010-01-01

Los procesos catalizados por lipasas han sido ampliamente investigados como alternativa para la producción de metil ésteres de ácidos grasos (fatty acid methyl ester, FAME) o biodiesel. El interés en este proceso radica en que a diferencia de los procesos convencionales catalizados con sales alcalinas, los procesos catalizados con lipasas permiten el uso de materias primas alternativas y de menor costo, tales como el aceite residual de fritura (waste frying oil, WFO). Pese a los l...

Iodophanus carneus and I. testaceus (Ascomycota-Pezizales: Independent taxonomic identity or synonymy? A study of their morphology and isozymes Iodophanus carneus e I. testaceus (ASCOMYCOTA-PEZIZALES: ¿Identidades taxonómicas independientes o sinonimia? Estudio morfológico e isoenzimático

Directory of Open Access Journals (Sweden)

Isabel E. Cinto

Full Text Available The aim of this study was to delimit two Iodophanus species: I. carneus and I. testaceus , based on morphological characteristics and electrophoretic patterns of their intracellular isozymes. Twenty monosporic strains were used, including five belonging to I. granulipolaris as a control. Fourteen isozyme systems were tested, and the five having the best resolution selected: aspartate aminotransferase, esterases, alkaline phosphatase, glutamate dehydrogenase, and superoxide dismutase. These analyses confirmed the similarity between I. carneus and I. testaceus , since they both produced the same band patterns, which were in turn different from the band pattern of I. granulipolaris. So, as we couldn´t find any character wich permit us to classify the isolated studied during this work in defferent species, we believe that I. testaceus shoul be consider as a synonym of I. carneus .El objetivo del presente trabajo fue la delimitación taxonómica de dos especies del género Iodophanus : I. carneus e I. testaceus a partir de caracteres morfológicos y de los patrones electroforéticos de isoenzimas intracelulares. Para ello se utilizaron veinte cepas monospóricas, cinco de las cuales pertenecientes a I. granulipolaris que fueron utilizadas como control. Se probaron catorce sistemas isoenzimáticos y se eligieron los cinco con mejor resolución: aspartato amino transferasa, esterasa, fosfatasa alcalina, glutamato dehidrogenasa y superóxido dismutasa. El análisis de los patrones isoenzimáticos corroboró la silimitud existente entre I. carneus e I. testaceus , ya que los patrones de bandas obtenidas para estas dos especies fueron iguales y diferentes de I. granulipolaris. Entonces, al no encontrar ningún caracter que nos permita separar a los aislamientos estudiados en este trabajo en dos especies distintas, proponemos a I. testaceus como un sinónimo de I. carneus.

Variaciones metabólicas, enzimáticas y electrolíticas en pacientes urolitiásicos sometidos a litotripsia extracorpórea por ondas de choque | Metabolic, enzymatic and electrolytic variations in urolithic patients undergoing extracorporeal shock wave lithotripsy

Directory of Open Access Journals (Sweden)

William Velásquez,

2017-10-01

Full Text Available La urolitiasis es una patología renal potencialmente obstructiva que puede requerir de tratamientos no invasivos como la litotripsia extracorpórea por ondas de choque (LEOC para devolver la función renal normal al paciente. El objetivo del presente estudio descriptivo-longitudinal fue evaluar las variaciones glucídicas, lipídicas, proteicas, enzimáticas y electrolíticas en pacientes urolitiásicos, antes y después del tratamiento con LEOC. Se estudiaron 45 pacientes, con diagnóstico de urolitiasis, atendidos en el Instituto Metropolitano Docente de Urología, Caracas. Para tal fin, se tomaron muestras sanguíneas, 2 horas antes y 24 horas después del sometimiento a las ondas de choque. Posteriormente, se obtuvieron los sueros por centrifugación, y se determinaron las concentraciones de los parámetros glucosa (GLU, colesterol (COL, triglicéridos (TRIG, proteínas totales (PT, albúmina (ALB, sodio, potasio, cloruro, calcio, fósforo, magnesio, globulinas (GLOB, y las actividades de las enzimas lactato deshidrogenada (LDH, creatina fosfoquinasa (CFQ, fosfatasa alcalina (FA, alanina aminotrasferasa (AlAT y aspartato aminotransferasa (AsAT. Los resultados obtenidos ponen de manifiesto que, posterior al tratamiento con LEOC, se observaron incrementos significativos en la actividad de la enzima LDH y disminuciones significativas en los parámetros GLU, potasio, calcio, fósforo y magnesio. Estos resultados permiten señalar que, probablemente, los efectos de la LEOC sobre el páncreas, hígado y vías urinarias, ocasionan traumatismo, inflamación y afectan los niveles séricos de glucosa y electrolitos y la actividad de la enzima LDH.

Influencia de especies forestales sobre la actividad de las enzimas fosfatasa ácida y proteasas en un suelo de bosque Influence of tree species on the activity of acid phosphatase and protease in a forest soil

Directory of Open Access Journals (Sweden)

Rl Defrieri

2008-12-01

Full Text Available Los distintos tipos de cubiertas vegetales y especialmente las especies arbóreas dominantes en los sistemas forestales, afectan las propiedades químicas y bioquímicas de los suelos. Dado que la concentración de algunos nutrientes en la hojarasca, entre ellos N y P, es considerada crítica para la descomposición del material vegetal, el objetivo de este trabajo ha sido estudiar a dos profundidades, la influencia del aporte de biomasa vegetal proveniente de especies forestales dominantes, sobre la actividad de las enzimas fosfatasa ácida y proteasas en un suelo de un monte del Parque Chaqueño Argentino. Se extrajeron muestras de suelo, ubicadas debajo de árboles de cuatro especies del monte, a dos profundidades (0-10 y 10-20 cm, en verano y en invierno sobre las que se determinaron las actividades enzimáticas mencionadas y algunas propiedades edáficas. Para todas las especies y en las dos épocas de muestreo se comprobó que los valores de las actividades de las dos enzimas y los parámetros edáficos analizados resultaron significativamente menores en las muestras a la profundidad de 10-20 cm. La influencia de las especies forestales sobre la actividad de las enzimas estudiadas se puso de manifiesto en el muestreo superficial en donde es mayor la influencia de la incorporación de los residuos orgánicos y en verano. En estas condiciones los valores de las actividades enzimáticas medidas debajo de cada especie oscilaron entre 1.600 y 900 μg p-nitrofenol g-1 h-1 para fosfatasa ácida y entre 850 y 450 mg tirosina g-1h-1 para proteasa. Para dos de las especies estudiadas se pudo vincular la magnitud de su aporte vegetal, su tasa de descomposición y los valores de N y P en hojas senescentes con la magnitud de la liberación de ambas enzimas al suelo según la especie. No se encontró asociación de la influencia de las especies con algunos parámetros edáficos analizados. Por lo tanto la actividad de las enzimas medidas en este trabajo

Proteasas alcalinas de una cepa nativa de Bacillus sp Alcalofílico Proteasas alcalinas de una cepa nativa de Bacillus sp Alcalofílico

Directory of Open Access Journals (Sweden)

A. Sáez Vega

2006-06-01

Full Text Available Se evaluó el efecto de cuatro fuentes de nitrógeno sobre la actividad enzimática de proteasas alcalinas, secretadas por una cepa nativa de Bacillus sp Alcalofílico, cultivada a diferentes concentraciones de LMF (Licor de Maíz Fermentado. El crecimiento de la cepa no es afectado por los pH de inoculación de 7,0; 8,5 y 9,5; en contraste con la actividad enzimática y producción de proteína verdadera, que tuvieron sus mejores resultados a pH inicial de 8,5. A este pH se evaluaron dos fuentes de nitrógeno orgánico (extracto de levadura y peptona y dos inorgánicos (NH4Cl y NaNO3The effect of four nitrogen sources on the enzymatic activity of alkaline proteases from a wild strain of Alkalophilic Bacillus sp cultivated to different concentrations from CSL (Corn Steep Liquor. was evaluated. The growth of the strain is not affected by pH of inoculation of 7,0, 8,5 and 9,5, in contrast to the enzymatic activity and true protein production, that had their better results to initial pH of 8,5. To this initial pH of 8,5; two organic nitrogen sources (yeast extract and peptone and two inorganic ones were evaluated (NH4Cl and NaNO3. With peptone the best enzymatic activity to a relation appeared to molar C/N between 1 and 2 was found. For the studied interval of % CSL (0,5 to 2% p/v the concentration of the CSL does not affect the enzymatic activity.

CAMBIOS EN LAS PROPIEDADES QUÍMICAS Y EN LA ACTIVIDAD DE LAS FOSFATASAS EN SUELOS CULTIVADOS CON MAÍZ DULCE (ZEA MAYS L. FERTILIZADOS CON VINAZA CHANGES IN CHEMICAL PROPERTIES AND PHOSPHATASES ACTIVITY IN SOILS UNDER SWEET CORN (ZEA MAYS L. CULTIVATION FERTILIZED WITH VINASSE

Directory of Open Access Journals (Sweden)

Magda Narváez Castillo

2010-12-01

Full Text Available Se evalúo el efecto de la aplicación de vinaza en las propiedades químicas y la actividad de las fosfatasas ácidas y alcalinas de dos suelos del Valle del Cauca, Colombia (Typic Argiudoll y Fluventic Haplustoll en condiciones controladas. Se establecieron seis tratamientos y cinco repeticiones distribuidos en un diseño completamente al azar, cuatro de ellos buscaban suplir los requerimientos de K+ del cultivo maíz dulce de la siguiente forma: T1, 100% del K+ con vinazas, T2 100% de K+ con KCl, T3 50% de K+ con Vinaza y 50% con KCl, y T4 75% del K+ con vinaza y 25% con KCl, T5 fue el testigo absoluto sin planta y T6 el testigo más planta. Las evaluaciones se realizaron en un periodo de 76 días y las muestras se tomaron entre 0-5 cm de profundidad. El pH, K+, B y relación Ca + Mg/K disminuyeron, muy significativamente (PIt was evaluated the effect of vinasse application on chemical properties and activity of acid and alkaline phosphatases of two soils of Valle del Cauca Colombia (Typic Argiudoll and Fluventic Haplustoll under controlled conditions. Six treatments and five replicates arranged in a completely randomized design were stablished; four of them, sought meet the requirements of K+ crop sweet corn as follows: T1, 100% of K+ with vinasse, T2 100% K+ with KCl T3 50% of K+ with vinasse and 50% KCl, and T4 75% of K+ with vinasse and 25% with KCl, T5 was the absolute control without plant and T6 the witness more plant. The evaluations were conducted over a period of 76 days and samples were taken from 0 - 5 cm deep. The pH, K+, B and Ca + Mg / K decreased significantly (P<0.01, while the contents of K+ and B were increased in both soils The P, Cu, Na and Fe increased differently in each soil. Alkaline phosphatase was significantly higher in the Fluventic Haplustoll and maximum activity occurred at 10 days after sowing (DAS; however, no significant differences between soils with reference to acid phosphatase and increased activity

Studies on the effect of vanillin (food additive on some metabolic reactions of the experimental animals

Directory of Open Access Journals (Sweden)

El-Massry, R. A.

1991-12-01

función hepática (S.GOT, S.GPT, alcalina y ácido fosfatasa así como las funciones renales se elevaron en comparación con el grupo control. La administración de vanillina redujo significativamente los lípidos totales de hígado y riñones. El bazo y corazón siguieron la misma tendencia pero con efecto moderado, mientras que el cerebro no se afectó. El colesterol total en hígado, riñones, bazo y corazón disminuyó significativamente, en tanto que en cerebro no se afectó. Los triglicéridos disminuyeron significativamente en hígado y bazo, mientras que no se alteraron en riñones y cerebro. También hubo una disminución significativa en la alta actividad de S.GOT, S.GPT, alcalina y fosfatasa acida y se alcanzaron valores muy próximos al nivel inicial. La administración de vanillina ejerció efectos anabólicos potentes para el metabolismo de proteínas como se demuestra de los resultados del ácido urónico y creatinina.

Desenvolvimento e análise de filme anti-reflexo de sulfeto de zinco para células solares

OpenAIRE

Ly, Moussa

2007-01-01

O aproveitamento da energia gerada pelo Sol, renovável e limpa é hoje, uma das alternativas energéticas mais promissoras para enfrentar o desafio energético do mundo. Para se fabricar uma célula solar de alta eficiência, precisa-se reduzir a refletância da superfície. O objetivo deste trabalho é estudar o desenvolvimento e análise de filmes anti-reflexo (AR) de sulfeto de zinco (ZnS) para células solares. Inicialmente, foi implementado e otimizado um processo de deposição por aquecimento resi...

Aprovechamiento de un LG-MgO para la obtención de carbonato básico de Mg de alta pureza

OpenAIRE

Ferré Monserrat, Ferran

2013-01-01

Los carbonatos básicos de magnesio, cuya fórmula general es xMgO·yCO2·zH2O, se sintetizan por hidrólisis alcalina del correspondiente carbonato de magnesio. Los carbonatos básicos de magnesio se han empleado recientemente como carga ignífuga en matrices poliméricas, mejorando notablemente el comportamiento de éstas en caso de incendio. Por otro lado, se han utilizado también como materiales para la captación y almacenamiento de CO2 a partir de MgO, reduciendo las emisiones de estos gases cont...

ELISA indireto para detecção de Ig G anti-vírus da doença de newcastle em soro de codorna

OpenAIRE

Daniela Duarte de Oliveira

2004-01-01

Foram desenvolvidos e comparados dois ELISAs para detecção de IgG de codorna (Coturnix coturnix japônica) contra o vírus da doença de Newcastle (VDN). Um deles utilizando anticorpo secundário de camundongo anti-IgG de codorna e o outro, conjugado anti-IgG de galinha. Para a produção de anticorpo secundário (IgG de camundongo anti-IgG de codorna), métodos de purificação de IgG de codorna foram avaliados utilizando 30 amostras de soros e gemas de ovos de codornas, confirmados posteriormente por...

Perfuração do colo por colite amebiana invasiva durante terapia anti-TNF para espondiloartrite♧

Directory of Open Access Journals (Sweden)

Juan Pablo Restrepo

2014-12-01

Full Text Available O bloqueio do TNF tem tido sucesso no tratamento de algumas doenças reumáticas, como a espondiloartrite. Relatam-se muitas complicações infecciosas com a terapia anti-TNF, principalmente infecções bacterianas, micobacterianas, virais e fúngicas. A Entamoeba histolytica é um protozoário extracelular que causa principalmente colite e abscesso hepático, sendo que a perfuração intestinal é uma complicação rara, com alta mortalidade. O TNF é considerado o principal mediador da imunidade celular contra a amebíase. Inicialmente, é quimiotático para a E. histolytica, potencializando sua adesão ao enterócito por meio da lectina galactose-inibível, e depois ativando os macrófagos para matarem a ameba pela liberação de NO; assim, o bloqueio do TNF poderia ser prejudicial, aumentando a virulência amebiana. Descreve-se o caso de uma mulher de 46 anos com espondiloartrite que apresentou uma perfuração do colo por colite amebiana invasiva durante uso de anti-TNF.

Obtainment of Hg-free Mn/Zn solutions from spent alkaline batteries; Obtencion de soluciones de Mn/Zn libres de Hg provenientes de pilas alcalinas gastadas

Energy Technology Data Exchange (ETDEWEB)

Perez-Nicolas, L.; Espinosa-Ramirez, I. [Instituto Politecnico Nacional, UPALM, Mexico, D.F. (Mexico)]. E-mail: lepeni@hotmail.com; Aguilar, M. [Instituto de Fisica, UNAM, Mexico, D.F. (Mexico); Palacios-Beas, E. [Instituto Politecnico Nacional, UPALM, Mexico, D.F. (Mexico)

2009-09-15

a la naturaleza, terminando en basureros al aire libre o contenedores donde son incinerados, contaminando el ambiente, los suelos y los mantos acuiferos del planeta. Por lo anterior, en el presente trabajo se estudia la obtencion de soluciones de Mn y Zn (Mn/Zn {>=} 1) libres de Hg a partir de pilas alcalinas gastadas, para su utilizacion en la sintesis de ferritas (Mn,Zn)Fe{sub 2}O{sub 4} por via humeda. Se analiza el efecto del medio disolvente (H{sub 2}SO{sub 4}/H{sub 2}O{sub 2}, HCl y HCl/NO{sub 3}), la temperatura y el tiempo sobre el porcentaje de disolucion de los metales presentes en el material electrodico, utilizando espectroscopia de absorcion atomica (AA) y difraccion de rayos X (DRX) para la caracterizacion. Los resultados de la investigacion indican que las mejores condiciones de disolucion son MD=H{sub 2}SO{sub 4}/H{sub 2}O{sub 2}, T=50 grados centigrados y t =30 min, donde se obtiene 94.1 y 90.7 % (w/w) de Mn y Zn respectivamente, con Mn/Zn = 1.51. Se determino que el contenido de mercurio es 3.91%, superior al declarado en las especificaciones de las pilas, que se recupera mediante disolucion con HCl/HNO{sub 3} en el solido remanente.

SÍNDROME DE HÍGADO GRASO EN UN CABALLO CRIOLLO COLOMBIANO

Directory of Open Access Journals (Sweden)

J. M. Cruz

2013-01-01

Full Text Available El objetivo de este trabajo fue discutir sobre los hallazgos clínicos, los exámenes de patología clínica realizados y su utilidad como indicadores de pronóstico en un pacien - te equino hembra con hígado graso. Se describe el caso de una yegua de raza criollo colombiano que ingresó al servicio de consulta externa del Hospital Veterinario Diego Villegas Toro de la Universidad de Caldas con signos de tránsito intestinal lento y cólico. Previamente, el veterinario remitente inició un tratamiento con fluidos y aceite cristal oral sin resultados satisfactorios. Se realizó un examen físico completo y pruebas paraclínicas (creatinina, BUN, albúmina, fibrinógeno, fosfatasa alcalina [FA], gamma glutamiltransferasa [GGT], alanina aminotransferasa [ALT], aspartato aminotransferasa [AST] y análisis de orina que sugirieron disfunción hepática y compactación del colon mayor. Se instauró un tratamiento dirigido a favorecer el peristaltismo y la eliminación fecal y se dispensó soporte para coadyuvar a la función hepática. Durante la hospita - lización se observaron signos clínicos compatibles con falla hepática, encefalopatía e compactación grave; finalmente, el equino murió. Durante la necropsia se confirmó compactación del colon mayor a nivel de la flexura esternal y peritonitis. El diagnóstico histopatológico fue hígado graso y peritonitis. Se concluye que la falla hepática puede asociarse con compactación del colon.

Cáncer anaplásico de tiroides: Reporte de caso

Directory of Open Access Journals (Sweden)

Miguel Pinto-Valdivia

2012-04-01

Full Text Available Mujer de 40 años, sin antecedente de patología tiroidea, con historia de enfermedad de cuatro semanas caracterizado por baja de peso, aumento rápido del tamaño de la glándula tiroides y dolor óseo generalizado. El examen físico mostró bocio multinodular no doloroso con múltiples ganglios cervicales. Los análisis de laboratorio mostraron anemia y niveles elevados de transaminasas, fosfatasa alcalina y deshidrogenasa láctica. El perfil tiroideo fue normal y los anticuerpos antiperoxidasa tiroidea fueron negativos. La biopsia por aspiración mostró carcinoma de tiroides. Se realizó una tiroidectomía total con disección amplia de los ganglios del cuello y en el estudio de anatomía patológica resultó un carcinoma anaplásico de tiroides. La evolución de la paciente fue desfavorable, falleciendo por insuficiencia respiratoria, secundaria a embolismo pulmonar. El carcinoma anaplásico de tiroides es poco frecuente, pero tiene una alta mortalidad. Las metástasis a distancia están asociadas a mal pronóstico.

Planteamiento y evaluación de las aplicaciones de los productos obtenidos en la hidrólisis alcalina de las virutas de cromo generadas durante el procesamiento del cuero

OpenAIRE

Díaz, Andrea; Jiménez, Juliana; Pérez, Monica; Narváez Rincón, Paulo César

2010-01-01

Considerando el concepto de maximizar la reutilización de residuos sólidos de curtiembres en diversos usos industriales y la minimización del impacto ambiental que estos generan, se evalúan algunas aplicaciones de los subproductos obtenidos por la hidrólisis alcalina de las “virutas de cromo”5 generadas durante la etapa de rebalado en el procesamiento del cuero. El hidróxido de cromo obtenido transformado en sulfato de cromo mo- nobasico puede reutilizarse en el procesamiento del cuero co...

A realização do teste anti-hiv no pré-natal: os significados para a gestante

OpenAIRE

Silva, Roberta Maria de Oliveira; Araújo, Carla Luzia França; Paz, Fatima Maria Trigo da

2008-01-01

O estudo teve por objetivo conhecer e analisar o significado da realização do teste anti-HIV no pré-natal para as gestantes. Trata-se de uma pesquisa com abordagem qualitativa e foi realizada em um Hospital Escola e em uma Maternidade do município do Rio de Janeiro. Como recurso técnico-metodológico utilizou-se o discurso do sujeito coletivo (DSC). Após a análise dos discursos verificamos que para as gestantes a realização do teste significa a possibilidade de prevenir a transmissão vertical ...

Aconselhamento em DST/Aids às gestantes que realizaram o teste anti-HIV na admissão para o parto: os sentidos de uma prática

Directory of Open Access Journals (Sweden)

Patrícia de Lima Fonseca

Full Text Available Foram analisadas as práticas e os sentidos do aconselhamento para gestantes submetidas ao teste anti-HIV na admissão para o parto, e para profissionais de saúde que atuam na assistência à maternidade em Salvador, Brasil. Foi realizado um estudo qualitativo em uma maternidade, com observação participante e entrevistas semiestruturadas com 13 puérperas sem diagnóstico prévio para o HIV e sete profissionais de saúde. Observou-se que o exame anti-HIV é realizado de forma compulsória, sem considerar a autonomia da gestante, e que o aconselhamento se limita a informar o diagnóstico e dar orientações no pós-teste somente àquelas cujos resultados foram positivos. Os sentidos que permeiam o exame, assim como o entendimento da experiência e os significados construídos pelas puérperas, sobretudo quando se descobrem positivas para o HIV, não são abordados pelos profissionais, que não se sentem capacitados para acolherem a subjetividade das pacientes.

Aconselhamento em DST/Aids às gestantes que realizaram o teste anti-HIV na admissão para o parto: os sentidos de uma prática

Directory of Open Access Journals (Sweden)

Patrícia de Lima Fonseca

2012-06-01

Full Text Available Foram analisadas as práticas e os sentidos do aconselhamento para gestantes submetidas ao teste anti-HIV na admissão para o parto, e para profissionais de saúde que atuam na assistência à maternidade em Salvador, Brasil. Foi realizado um estudo qualitativo em uma maternidade, com observação participante e entrevistas semiestruturadas com 13 puérperas sem diagnóstico prévio para o HIV e sete profissionais de saúde. Observou-se que o exame anti-HIV é realizado de forma compulsória, sem considerar a autonomia da gestante, e que o aconselhamento se limita a informar o diagnóstico e dar orientações no pós-teste somente àquelas cujos resultados foram positivos. Os sentidos que permeiam o exame, assim como o entendimento da experiência e os significados construídos pelas puérperas, sobretudo quando se descobrem positivas para o HIV, não são abordados pelos profissionais, que não se sentem capacitados para acolherem a subjetividade das pacientes.

Construcción de un vector para la integración cromosomal de un gen de fitasa de Bacillus licheniformis

Directory of Open Access Journals (Sweden)

Maria Teresa Fernández

2011-07-01

Full Text Available Las fitasas son una clase especial de fosfatasas que catalizan la hidrólisis secuencial del fitato. La incapacidad de las plantas para utilizar el fósforo a partir de los fitatos del suelo es debido a la baja actividad de fitasas en sus raíces. Los microorganismos del suelo juegan un importante papel en los procesos que afectan la trans- formación de los compuestos fosforados. Muchos de ellos pueden solubilizar el fósforo a partir de los fitatos, mediante la liberación de fitasas. Este proceso permite la movilización del fósforo hacia las plantas y un mejor aprovechamiento de este nutriente. Sin embargo, muchas bacterias carecen de los genes que codifican para estas enzimas, lo que disminuye la disponibilidad de este elemento en el suelo. Una alternativa es mejorar las rizobacterias en cuanto a su capacidad de solubilizar los fitatos del suelo, mediante la transformación genética. En este trabajo el gen phyL de Bacillus licheniformis fue clonado en el vector de liberación suicida pJMT6 (vector derivado del sistema pUT/mini Tn5. La construcción recombinante que contiene un marcador de selección no antibiótico, fue transformada en Escherichia coli CC118λpir. Un clon transformante (F16 fue seleccionado y posteriormente caracterizado. Estos resultados constituyen un primer paso para desarrollar rizobacterias promotoras del crecimiento mejoradas en cuanto a la producción de actividad fitasa recombinante, como alternativa para reducir la contaminación ambiental y mejorar la productividad de los cultivos.

IN VITRO STUDY OF ANTI-INFLUENZA ACTIVITY OF PARA-AMINOBENZOIC ACID AND PROSPECTS OF NASAL DRUG DEVELOPMENT

Directory of Open Access Journals (Sweden)

Nefedova L.

2017-06-01

Full Text Available is a vitamin-like substance, para-aminobenzoic acid (PABA. The aim of this work is to study antiviral activity of para-aminobenzoic acid, ε-aminocaproic acid, and their mixture against influenza virus, to evaluate their cytotoxic effect and to calculate their selectivity index by in vitro methods. Materials and Methods. For evaluation of cytotoxic concentration (CC50 of the substance tested, we used MDCK cell culture. For determination of anti-influenza activity of PABA, ε-АCA, and their mixture under conditions of in vitro experiment, we used 24-hour passaged MDCK culture cells of dog’s kidney, influenza virus strain А/FM/1/47 (H1N1, infected titer of which in MDCK culture cells of was between 3.0 and 9.0 lgID50. Results. As a result of our studies, we have determined that PABA in the concentration range studied from 10 to 1,000 ug/ml did not have any cytopahtic effect in MDCK culture cells. We calculated selectivity index (SI for PABA, that was equal SI=128205, this fact confirm high level of therapeutic safety of this substance. The result of our experiments demonstrate that anti-influenza activity of PABA solution is almost 100 times higher than that of “ACA”, furthermore in our in vitro experiment the mixture of PABA and ε-aminocaproic acid (in ratio 1:100 demonstrates a synergetic effect. In addition, for combination of PABA and εАКК (1:100 we calculated fractional inhibitory coefficient FIC=0.79, that indicates to moderate synergetic effect of these substances. Conclusions. As a result of our in vitro studies, we have determined a high-level antiviral activity of para-aminobenzoic acid against influenza virus, and in combination with ε-aminocaproic acid it demonstrated a synergetic effect. We have determined that PABA does not exhibit any cytotoxic effect on MDCK culture cells in the concentration range studied and demonstrates high level of therapeutic safety.

Screening of filamentous fungi for production of enzymes of biotechnological interest Seleção de fungos filamentosos para a produção de enzimas de interesse biotecnológico

Directory of Open Access Journals (Sweden)

Luis Henrique S. Guimarães

2006-12-01

examinados quanto à sua capacidade de produzir xilanase, glicose-oxidase, fosfatase alcalina, fosfatase ácida, fitase, pectinase e amilase. Entre estes, vinte e três isolados exibiram potencial enzimático. Xilanases produzidas por dois destes isolados (Aspergillus caespitosus e A. phoenicis mostraram bons resultados no biobranqueamento da polpa de celulose. Entre dezessete isolados, pelo menos três produziram altos níveis de glicose oxidase, sendo Rhizopus stolonifer e Aspergillus. versicolor os melhores produtores. Aspergillus caespitosus, Mucor rouxii e nove outros ainda não identificados foram os melhores produtores de fosfatases em fermentação submersa. Pectinase foi produzida preferencialmente por IF II e C-8 seguida por Rhizopus stolonifer. Níveis significantes de amilases foram produzidos por Paecilomyces variotii e Aspergillus phoenicis. Um notável produtor de diversas enzimas foi Rhizopus microsporus var. rhizopodiformis, que produziu altos níveis de amilase, fosfatase alcalina e ácida e pectinase. Algumas estruturas morfológicas deste fungo estão sendo ilustradas por microscopia de luz e microscopia eletrônica de varredura. Esse estudo contribui para catalogar fungos isolados do estado de São Paulo e fornece informações adicionais para pesquisas futuras sobre o potencial industrial destes microrganismos produtores de enzima e eventualmente também metabólitos secundários com atividade antimicrobiana e antiparasitária.

Análisis de algunas variables hematológicas, bioquímicas y clínicas en la anemia drepanocítica. Resultados preliminares Analysis of some hematological, biochemical and clinical variables in cases of sickle cell anemia.Preliminary results

Directory of Open Access Journals (Sweden)

Marianela Estrada del Cueto

2010-08-01

Full Text Available Se estudiaron 59 pacientes mayores de 18 años con anemia drepanocítica distribuidos en: grupo 1 (23 sin síndrome torácico agudo (STA, priapismo, ni úlceras maleolares; grupo 2 (39 que han presentado alguna de las complicaciones anteriores. En el grupo 2 se observó disminución significativa de la hemoglobina (Hb y la Hb fetal, así como aumento de plaquetas, deshidrogenasa láctica (LDH, aspartato aminotransferasa (AST, alanino aminotransferasa (ALT, bilirrubina total (BT, directa (BD e indirecta (BI, fosfatasa alcalina (FAL, creatin kinasa-MB (CK-MB y de la frecuencia de accidente vascular encefálico. La frecuencia de STA, úlceras maleolares y priapismo en el grupo 2, fue del 83,3; 33,3 y 50 %, respectivamente. El análisis de regresión lineal múltiple en el total de enfermos tomando como variable dependiente la LDH, mostró correlación negativa significativa para la Hb y el Hto y positiva para creatinina, AST, ALT, BT, BD, BI, FAL y CK-MB. La frecuencia de hipertensión pulmonar fue del 18,2 % (n= 10. Estos estudios pueden ser útiles en el diagnóstico precoz de complicaciones relacionadas con la hemólisis.A total of 59 patients aged over 18 with sickle cell anemia were studied distributed in: group 1 (23 without acute thoracic syndrome (ACS, priapism and malleolus ulcers; group 2 (39 with some of above complications. In group 2 there was a significant decrease of hemoglobin (Hb and the fetal Hb and a platelet increase, lactic dehydrogenase (LDH, aspartate aminotransferase (AST, alanine aminotransferase (ALT, total bilirubin (TB, alkaline phosphatase (APH, creatine kinase-MB (ck-MB and of encephalic stroke frequency. The frequency of AST, malleolus ulcers and priapism in group 2 was of 83.3 %, 33.3 % and 50 %, respectively. The multiple linear regression analysis in all patients considering the LDH as a negati ve correlation significant for Hb and the Hto and positive for creatinine, AST, ALT, TB, DB, IB, APH and CK-MB. The

Reporte de insuficiencia renal producida por Leptospira Interrogans Serovar Pomona en Colombia

Directory of Open Access Journals (Sweden)

Fortunato Ospino

1988-06-01

Full Text Available Se describe la presentación de un caso de leptospirosis producida por L. pomona en un paciente de 20 años de edad, trabajadora habitual en un molino de arroz y caracterizado por un síndrome de insuficiencia renal y hepática. El cuadro clínico consistió principalmente en malestar general, anorexia, vómito, fiebre, diarrea, ictericia y hemorragias subconjuntivales. Los exámenes de laboratorio mostraron alteraciones, en los niveles de nitrógeno ureico, creatinina, depuración de creatinina, concentración de cloro, fosfatasa alcalina y bilirrubina directa lo cual podría indicar un daño en el funcionamiento hepático y renal. Las alteraciones en la velocidad de sedimentación, hemoglobina, hematocrito y leucocitos (Tabla 1 explican la anemia e infección ocasionada por la L. pomona en la paciente. El diagnóstico de leptospirosis fue confirmado por el aislamiento del microorganismo de la orina a pesar de no haber detectado anticuerpos en el suero de la paciente. Se pone en evidencia la necesidad de realizar siempre el diagnóstico diferencial con leptospirosis en todo síndrome hepatorrenal, especialmente en los casos con antecedentes epidemiológicos o que por la sintomatología clínica se sospeche la infección.

Estabilidade do comportamento anti-social na transição da infância para a adolescência: uma perspectiva desenvolvimentista Stability of antisocial behavior on the infancy-adolescence transition: a developmental perspective

Directory of Open Access Journals (Sweden)

Janaína Pacheco

2005-04-01

Full Text Available O termo anti-social tem sido amplamente utilizado na literatura científica para descrição de problemas de comportamento não específicos, como comportamentos delinqüentes, agressividade e oposicionismo. O objetivo desse estudo é descrever e discutir o conceito de comportamento anti-social, como um indicador de transtornos mentais específicos e de algumas categorias de problemas comportamentais. Para isso, examinamos a relação entre o comportamento anti-social e o Transtorno Desafiador Opositivo, o Transtorno da Conduta, o Transtorno de Déficit de Atenção e Hiperatividade e o Transtorno de Personalidade Anti-social. Além disso, discute-se também os fatores que contribuem para a estabilidade desse comportamento na transição da infância para a adolescência e os prejuízos decorrentes ao longo do desenvolvimento. Propõe-se a ampliação das discussões conceituais acerca dos transtornos mentais, utilizando-se categorias mais amplas, como a de comportamento anti-social.The term antisocial is widely used in the literature to describe non-specific behavior problems such as delinquent behavior, aggressiveness, and oppositionist behavior. The aim of the present study was to describe and to discuss the concept of antisocial behavior as an indicator of specific mental disorders such as Attention-deficit and Hyperactivity Disorder, Conduct Disorder, Oppositional Defiant Disorder, and Antisocial Personality Disorder. Also, we discuss the factors that contribute to the stability of such behaviors in the transition from childhood to adolescence and the losses incurred throughout development. A recommendation is made to broaden conceptual discussions about mental disorders using wider categories such as antisocial behavior.

A importância da eletroforese de hemoglobina na orientação genética para síndrome falciforme

Directory of Open Access Journals (Sweden)

Júlio Boschini Filho

2016-10-01

Full Text Available Introdução: As hemoglobinopatias são distúrbios genéticos, em geral de herança recessiva. O diagnóstico precoce possibilita o aconselhamento genético com finalidade de orientar a respeito do planejamento familiar, ajudando a entender como a hereditariedade pode colaborar para a ocorrência ou risco de recorrência de doenças genéticas, como é o caso da síndrome falciforme. Objetivo: realizar a orientação e o encaminhamento para aconselhamento genético dos pacientes que foram diagnosticados com síndrome falciforme e em seus familiares, visando a prevenção da recorrência da doença nas próximas gerações. Casuística e método: consistiu na entrevista e orientação para os portadores da síndrome falciforme. Usamos como método de diagnóstico a eletroforese alcalina (pH 8,6 para caracterização do tipo de hemoglobinopatia. Resultado: observamos que ainda existe pouca compreensão dos pacientes e dos seus familiares sobre sua patologia e mecanismo de transmissão. Vimos que 25% dos familiares de primeiro não possuem conhecimento sobre portar um tipo de síndrome falcêmica. Conclusão: o desconhecimento da doença impede uma prevenção eficaz, o que acarreta altos custos para o Governo e para Saúde na questão do tratamento das Síndromes Falciformes, permitindo recorrência da morbidade. Por esse motivo, é fundamental que esse tipo de serviço seja amplificado.

Evaluación de los reactivos hemoclasificadores monoclonales cubanos HEMO CIM ANTI-A y HEMO CIM ANTI-B en pacientes VIH/SIDA Evaluation of the Cuban anti-A Hemo CIM and anti-B Hemo CIM monoclonal hemoclassifiers in HIV/AIDS patients

Directory of Open Access Journals (Sweden)

Ananidia Rivero

2000-12-01

Full Text Available Se describe un estudio realizado con los hemoclasificadores monoclonales cubanos Hemo CIM anti-A y anti-B producidos por el Centro de Inmunología Molecular (CIM y comercializados por CIMAB S.A, para ser evaluados en pacientes VIH/SIDA. Se analizaron un total de 130 pacientes en el Servicio de Transfusiones del Instituto "Pedro Kourí". Se utilizaron en paralelo los antisueros policlonales de producción nacional (Laboratorios Betera y los reactivos monoclonales comerciales producidos y donados por International Blood Group Reference Laboratory; Bristol, UK. Se demostró que los reactivos Hemo CIM anti-A y Hemo CIM anti-B se comportaron de forma similar a sus homólogos comerciales y policlonales. Al comparar la efectividad de la aglutinación para los 3 reactivos pudimos comprobar que con el reactivo monoclonal, Hemo CIM anti-A y anti-B se obtuvieron los mejores resultados expresados en crucesA study conducted with the Cuban anti-A and anti-B Hemo CIM monoclonal hemoclassifiers produced by the Center of Molecular Immunology (CMI and commercialized by CIMAB S.A. to be evaluated in HIV/AIDS patients is described. 130 patients were analyzed at the Service of Transfusions of "Pedro Kouri" Institute. The polyclonal antisera of national production (Betera Laboratories and the commercial monoclonal reagents produced and donated by the International Blood Group Reference Laboratory; Bristol, UK, were used at the same time. It was proved that the anti-A Hemo CIM and anti-B Hemo CIM reagents behaved similarly to their commercial and polyclonal homologues. On comparing the effectiveness of the agglutination for the 3 reagents, we were able to verify that the best results expressed in crosses were obtained with the anti-A and anti-B Hemo CIM monoclonal reagents

CARACTERIZACIÓN DE IGYS ANTI-LECTINA DE Salvia bogotensis Y SU APLICACIÓN EN ESTUDIOS CITOQUÍMICOS PARA LA DETECCIÓN DEL ANTÍGENO TN

Directory of Open Access Journals (Sweden)

Nohora Vega

2010-02-01

Full Text Available Las inmunoglobulinas aisladas de la yema de huevo (IgY son muy utilizadas actualmente en diversos campos de las ciencias biológicas, dadas sus ventajas frente a las IgG séricas de mamíferos. En un trabajo previo establecimos las condiciones de obtención de IgY dirigidas contra la lectinas de Salvia bogotensis; su utilización en estudios inmunocitoquímicos requiere conocer sus principales características moleculares y las condiciones para la interacción IgY-lectina. La lectina de Salvia bogotensis (SBoL reconoce específicamente el antígeno Tn, marcador tumoral en muchos tipos de cáncer, pero se requieren herramientas adicionales para evidenciar esta interacción a nivel celular. Dada la disponibilidad de IgY anti-lectina de S. bogotensis, se realizó este trabajo con el objeto de caracterizar molecularmente estas IgY y evaluar su utilización en estudios inmunocitoquímicos para la detección del antígeno Tn en células tumorales. A las IgY purificadas se les determinó su punto isoeléctrico, peso molecular y contenido de carbohidratos. Para establecer la especificidad de interacción IgY-SBoL se obtuvieron lectinas homólogas y heterólogas y se ensayaron por ELLSA. La detección del antígeno Tn en las líneas celulares MCF-7 y HeLa con la lectina y las IgYs marcadas con biotina o peroxidasa se realizó por CELISA e inmunocitoquímica. Los resultados mostraron que los anticuerpos IgY anti-SBoL son una herramienta de una alta sensibilidad para los ensayos de reconocimiento específico del antígeno Tn.

Anti-Leptospira spp. antibodies in captive animals in Paraíba State

Directory of Open Access Journals (Sweden)

Arthur Brasil

2013-12-01

Full Text Available The aim of this survey was to verify the occurrence of anti-Leptospira spp. antibodies in captive animals in the Parque Zoobotânico Arruda Câmara, João Pessoa, Paraíba State, Northeastern Brazil. Blood samples were collected from 49 animals: 26 mammals of the species Sapajus libidinosus, Cebus flavius, Saimiri sciureu, Coendu sp., Pseudalopex vetulus, Leopardus pardalis, Leopardus tigrinus, Galactitis vitata, Eira barbara, Nasua nasua, Tayassu tajacu and Ratus norvegicus; 10 birds of the species Penelope jacucaca, Pavo cristatus, Anodorhynchus hyacinthinus, Ara chlorothpterus, Pionites leucogaster, Polyborus plancus, Geranoaetus melanoleucus and Urubitinga urubitinga; and 13 reptiles of the species Caiman latirostris, Paleosuchus trigonatus, Caiman crocodilus, Tupinabis merinae, Tupinambis teguixin, Boa constrictor, Corallus hortulanus, Python molurus, Bufocephala vanderhaegei, Geochelone denticulata and Geochelone carboraria. Sera were examined by the microscopic agglutination teste (MAT using 24 serovars as antigens and cut-off point of 1:100. One ocelot (Leopardo pardalis presented positive reaction for the Icterohaemorrhagiae serovar with titer of 100, however, it did not show any clinical sign of the infection. Sinantropic rodents are the main reservoirs of this serovar, which suggests the need of maintenance and continuous evaluation of rodent control programs.

Telopéptido carboxilo terminal del colágeno tipo I (b-CTX sérico y compromiso óseo en la insuficiencia renal crónica Serum b-Type I collagen carboxyterminal telopeptide (b-CTXs and bone involvement in chronic renal failure

Directory of Open Access Journals (Sweden)

Beatriz Oliveri

2005-08-01

Full Text Available La osteodistrofia renal (ODR se caracteriza por alteraciones óseas. Se evaluaron métodos bioquímicos alternativos a la biopsia ósea en pacientes renales para determinar cambios rápidos del remodelamiento óseo en 43 pacientes predialíticos (PD y 49 hemodializados (HD. Los PD presentaron fosfatemia, fosfatasa alcalina ósea (FAO, hormona paratiroidea intacta (PTHi y b-telopéptido carboxilo terminal del colágeno tipo I (bCTXs mayores y clearence de creatinina (Ccr menores (p40 ml/min. En PD, bCTXs (pAn increase in parathyroid hormone (PTH levels in chronic renal failure (CRF induces bone abnormalities known as renal osteodystrophy (ROD. The aim of the present study was to evaluate alternative biochemical methods to bone biopsy, to evaluate changes in bone remodeling in renal patients. Intact PTH (iPTH and bone markers were measured in 43 predialysis (PD, 49 hemodialysis patients (HD and 185 controls. bCTXs, bone alkaline phosphatase (bAL, iPTH were higher and creatinine clearance (Ccr was lower in PD and HD compared with controls (p40 ml/min. bCTXs (p<0.05 in PD and bCTXs and bAL in HD patients were higher than controls, even when iPTH was within normal range (<65 pg/ml. Patients with severe secondary hyperparathyroidism showed higher bone markers than patients with normal or moderately increased iPTH (p<0.001. These results suggest that even when there is no increase in iPTH, bone remodeling increases (possibly due to other factors exhibiting higher bone resorption, and bCTXs would seem to be an adequate non-invasive tool to assess early bone changes in CRF and prevent future fractures. Bone marker measurements in ROD would be useful to identify patients who may require bone biopsy. However, further studies comparing both methods must be performed before replacing bone biopsy with serum b-CTX.

Efecto del proceso de fusión y envejecimiento en la síntesis de zeotipos a partir de cenizas volantes

Directory of Open Access Journals (Sweden)

Jeimer Alexander Lizcano-Cabeza

2015-01-01

Full Text Available En este estudio, se evalúa el uso de una muestra de cenizas volantes de TERMOPAIPA (Boyacá, Colombia como material de partida para la síntesis de zeotipos a escala de laboratorio a través de su transformación por fusión alcalina, utilizando NaOH sólido o disuelto en agua, seguida por envejecimiento durante 6, 12 y 24 h en condiciones estáticas o de ultrasonido previo al tratamiento hidrotérmico durante 6, 12 y 24 h de reacción. Los datos experimentales revelan que el método, el estado de fusión alcalina, la temperatura y el tiempo de reacción afectan fuertemente el zeotipo a ser sintetizado. Se sintetizaron zeotipos sódicos bajos en sílice, los cuales incluyen zeolita NaP1, faujasita, y trazas de sodalita. La síntesis de zeotipos a partir de cenizas volantes representa una interesante alternativa para la mitigación del problema ambiental, asociado a la disposición de residuos industriales. Por lo tanto, los zeotipos a base de cenizas volantes sintetizados bajo óptimas condiciones experimentales pueden ser utilizados en diversas aplicaciones para el tratamiento ambiental de residuos.

Anti-topoisomerase drugs as potent inducers of chromosomal aberrations

Directory of Open Access Journals (Sweden)

Loredana Bassi

2000-12-01

Full Text Available DNA topoisomerases catalyze topological changes in DNA that are essential for normal cell cycle progression and therefore they are a preferential target for the development of anticancer drugs. Anti-topoisomerase drugs can be divided into two main classes: "cleavable complex" poisons and catalytic inhibitors. The "cleavable complex" poisons are very effective as anticancer drugs but are also potent inducers of chromosome aberrations so they can cause secondary malignancies. Catalytic inhibitors are cytotoxic but they do not induce chromosome aberrations. Knowledge about the mechanism of action of topoisomerase inhibitors is important to determine the best anti-topoisomerase combinations, with a reduced risk of induction of secondary malignancies.As topoisomerases de DNA catalisam alterações topológicas no DNA que são essenciais para a progressão do ciclo celular normal e, portanto, são um alvo preferencial para o desenvolvimento de drogas anticâncer. Drogas anti-topoisomerases podem ser divididas em duas classes principais: drogas anti-"complexos cliváveis" e inibidores catalíticos. As drogas anti-"complexos cliváveis" são muito eficazes como drogas anticancerígenas, mas são também potentes indutores de aberrações cromossômicas, podendo causar neoplasias malignas secundárias. Inibidores catalíticos são citotóxicos mas não induzem aberrações cromossômicas. Conhecimento a respeito do mecanismo de ação de inibidores de topoisomerases é importante para determinar as melhores combinações anti-topoisomerases, com um reduzido risco de indução de neoplasias malignas secundárias.

Prevalence of anti-pestivirus antibodies and risk factors in dairy goats from the semiarid region of Paraíba State, Northeastern Brazil

Directory of Open Access Journals (Sweden)

Maria Luana Cristiny Rodrigues Silva

2014-06-01

Full Text Available t The aim of this survey was to determine the prevalence of anti-pestivirus antibodies in dairy goats from the semiarid region of the Paraíba state, Northeastern Brazil, as well as to identify risk factors associated with the flock-level prevalence. A total of 1,092 dairy goats from 110 flocks randomly selected in the county of Monteiro, Paraíba state, during March 2009 to March 2010, were used. In each selected flock a epidemiological questionnaire was applied to verify the occurrence of possible factors that could be associated with the flock-level prevalence. For the serological diagnosis of Pestivirus infection the serum neutralization test, using the BVDV-1 NADL strain, was carried out. Flock-level prevalence was 6.36% (95% CI = 2.60% – 12.67% and animal-level prevalence was 0.82% (95% CI = 0.38% – 1.56%. Not to perform vermifugation (odds ratio = 10.49; p = 0.035 and to perform navel cut and disinfection (odds ratio = 12.73; p = 0.034 were identified as risk factors. These results indicate viral circulation in dairy goats in the semiarid region of the Paraíba state.

Anti-M causing delayed hemolytic transfusion reaction

International Nuclear Information System (INIS)

Alperin, J.B.; Riglin, H.; Branch, D.R.; Gallagher, M.T.; Petz, L.D.

1983-01-01

A 52-year-old gravida 1, para 1 woman with M- red cells experienced a delayed hemolytic transfusion reaction and exhibited an anti-M antibody following the infusion of four units of M+ red cells. Measurements of erythrocyte survival using 51 Cr-labeled donor M+ and M- red cells and in vitro studies of monocyte-macrophage phagocytosis of sensitized reagent red cells implicate anti-M in the pathogenesis of hemolysis

Identificación de nuevos reguladores del ciclo celular en Schizosaccharmoyces Pombe

OpenAIRE

Chica Balaguera, Nathalia

2014-01-01

[ES] Los complejos CDK-ciclina controlan el ciclo celular eucariótico y en el caso de S. pombe una única CDK, llamada cdc2, es suficiente para regularlo. Los niveles de la CDK varían a lo largo del ciclo celular y a su vez confiere direccionalidad a los eventos que lo componen. Uno de los sistemas de control de los niveles de actividad mas estudiado en este organismo, comprende la activación e inactivación de la CDK por parte de la quinasa Wee1 y la fosfatasa Cdc25 respectivamente, durante la...

Propriedades de pasta de amidos de arroz nativo e acetilados

OpenAIRE

Bartz,Josiane; Madruga,Karina Medeiros; Klein,Bruna; Pinto,Vânia Zanella; Dias,Álvaro Renato Guerra

2012-01-01

O amido de arroz apresenta características favoráveis a muitas aplicações industriais; no entanto, a natureza hidrofílica do amido na forma nativa pode apresentar algumas limitações para determinados tipos de processamento. Neste estudo, amido de arroz com médio teor de amilose foi acetilado sob catálise alcalina em duas condições reacionais para produzir acetatos de amido com diferentes graus de substituição (GS). A introdução de grupos acetila ao amido foi confirmada por espectroscopia de i...

Pasting properties of native and acetylated rice starches

OpenAIRE

Bartz, Josiane; Madruga, Karina Medeiros; Klein, Bruna; Pinto, Vânia Zanella; Dias, Álvaro Renato Guerra

2012-01-01

O amido de arroz apresenta características favoráveis a muitas aplicações industriais; no entanto, a natureza hidrofílica do amido na forma nativa pode apresentar algumas limitações para determinados tipos de processamento. Neste estudo, amido de arroz com médio teor de amilose foi acetilado sob catálise alcalina em duas condições reacionais para produzir acetatos de amido com diferentes graus de substituição (GS). A introdução de grupos acetila ao amido foi confirmada por espectroscopia de i...

Effects of repeated extracorporeal shock wave in urinary biochemical markers of rats Avaliação dos fatores bioquímicos urinários de risco para nefrolitíase em ratos submetidos à aplicação repetida de ondas de choque eletro-hidráulicas

Directory of Open Access Journals (Sweden)

Márcio Carvalho

2009-12-01

Full Text Available PURPOSE: To access the effect of repeated extracorporeal shock wave (ESW on urinary biochemical markers METHODS: 20 rats were assigned for ESW (Direx Tripter X1® - 14 KV to one of two groups: G1 (n=10 one ESW; G2 (n=10 two ESWs within a 14-day interval. Within the twenty-four hour period before and after the application of shock waves, the animals were placed in metabolic cages for 24 hour urine collection. The ph, creatinine, sodium, potassium, chlorides, calcium, magnesium, phosphorus, oxalates, alkaline phosphatase and citrates were measured. Twenty-four hours after the material was collected for urinary determination, the animals underwent nephrectomy of the kidney submitted to the ESW applications and were, then, sacrificed. The kidneys were processed for hispatological examination. RESULTS: Small variations in the biochemical markers were found in both groups, with no significant differences between the values obtained either prior to or following the ESW applications, except for citrate and alkaline phosphatase. Citraturia decreased significantly in group 2, following the second ESWL application (24.8 ± 3.0 mg/day after the first ESWL vs. 15.3 ± 2.2 mg/day after the second ESWL; p OBJETIVO: Avaliar os efeitos renais das ondas de choque eletro-hidráulicas (OCEH, utilizando como parâmetros marcadores bioquímicos urinários. MÉTODOS: Foram utilizados 20 ratos machos, EPM - Wistar, distribuídos aleatoriamente em dois grupos: G1 (n=10 Animais submetidos a uma sessão de OCEH. G2 (n=10 Animais submetidos a duas sessões de OCEH separadas por um intervalo de 14 dias. Para coleta da urina os animais foram mantidos em gaiolas metabólicas 24 horas antes e depois da aplicação das OCEH. Foram medidos o pH, a creatinina, sódio, potássio, cloretos, cálcio, magnésio, fósforo, oxalato, fosfatase alcalina e citrato. Vinte e quatro horas após a coleta da urina os animais foram submetidos à nefrectomia do rim envolvido no experimento e, em

[In Process Citation].

Science.gov (United States)

Gonçalves Schemitt, Elizângela; Raskopf Colares, Josieli; Minuzzo Hartmann, Renata; Morgan-Martins, María Isabel; Marroni, Cláudio Augusto; Tuñón, M Jesús; Possa Marroni, Norma

2016-03-25

Introducción: la insuficiencia hepática fulminante (IHF) es un síndrome clínico poco frecuente, que se caracteriza por una disfunción hepática severa y repentina. La tioacetamida (TAA) es una hepatotoxina cuya administración puede inducir necrosis centrolobulillar en las células hepáticas y aumentar la formación de especies reactivas de oxígeno y la peroxidación lipídica en ratas. La glutamina es un precursor para la síntesis de glutatión. Objetivo: el objetivo del estudio es evaluar los efectos antioxidantes de la glutamina en un modelo de rata de IHF inducida por TAA. Métodos: ratas macho Wistar se dividieron en cuatro grupos de acuerdo con el tratamiento y el tiempo de evaluación: control, glutamina (25 mg/kg), tioacetamida (400 mg/kg) y tioacetamida más glutamina. Los animales se evaluaron después de 24, 36 y 48 horas. Se recogieron muestras de sangre para el análisis de los niveles de aspartato aminotransferasa (AST), alanina aminotransferasa (ALT), fosfatasa alcalina (AP), bilirrubina total (TB) y creatinina (CRE), y muestras de hígado para evaluar la peroxidación lipídica, las sustancias reactivas al ácido tiobarbitúrico (TBARS), la actividad de las enzimas antioxidantes superóxido dismutasa (SOD), glutatión peroxidasa (GPx), catalasa (CAT) y glutatión S-transferasa (GST). Además se midieron mediante inmunohistoquímica el factor nuclear kappa N (NF-κB), el fator de necrosis tumoral (TNF-α) y la óxido nítrico sintasa inducible (iNOS). Resultados: la TAA causó alteraciones en los parámetros bioquímicos e histológicos, y el aumento de los marcadores del proceso inflamatorio. Los niveles de TBARS y la actividad de SOD y GST fueron significativamente inferiores en los grupos de glutamina en comparación con TAA. La actividad de CAT se incrementó en los animales tratados con glutamina en comparación con la TAA. La actividad GPx también fue menor a las 36 y 48 h en los animales tratados com glutamina. El daño tisular y

Avaliação da hemorragia feto-materna em puérperas com indicação para ministração de imunoglobulina anti-D Evaluation of fetomaternal hemorrhage in postpartum patients with indication for administration of anti-D immunoglobulin

Directory of Open Access Journals (Sweden)

Eduardo Baiochi

2005-10-01

Full Text Available Avaliamos a ocorrência da hemorragia feto-materna entre 343 puérperas que receberiam profilaxia da aloimunização Rh com emprego de imunoglobulina anti-D. Realizamos o teste de roseta para triagem dos casos que necessitariam determinação quantitativa do volume de sangue fetal transferido para circulação materna, que foi então apurado pelo teste de Kleihauer-Betke (K-B. O teste de roseta apresentou resultado positivo em 22 casos (6,4%. Em cinco dessas amostras o teste de K-B não apontou hemorragia feto-materna (falso positivo do teste de roseta de 1,45% e noutra a leitura do teste não foi conclusiva. Tivemos oito casos com volume apurado de hemorragia feto-materna This study evaluated fetomaternal hemorrhage (FMH in 343 postpartum patients who required prophylaxis of Rh alloimmunization with anti-D immunoglobulin. The rosette test was applied to screen for patients needing quantitative determination of fetal blood transferred from the maternal circulation, which was then measured by the Kleihauer-Betke test (K-B. The rosette test was positive in 22 cases (6.4%. In five of these cases, K-B did not show fetomaternal hemorrhage (a 1.45% false-positive rate for the rosette test, and in one case the test was inconclusive. There were 8 cases with FMH 30ml (0.58%, requiring a supplementary dose of anti-D. The study concludes that following the rosette test, additional evaluation of FMH using a quantitative test was unnecessary in 93.6% of the cases.

Perfil sérico da fosfatase alcalina após o reparo de defeitos osteocondrais com células mononucleares da medula óssea e proteína morfogenética óssea em coelhos

Directory of Open Access Journals (Sweden)

Luiz Augusto Souza

2011-08-01

Full Text Available http://dx.doi.org/10.5007/2175-7925.2011v24n4p103 Verificou-se a atividade da fosfatase alcalina sérica em resposta ao reparo de defeitos osteocondrais em vinte e quatro coelhos da raça Nova Zelândia. Distribuídos em três grupos, o controle (GC, o tratado com células mononucleares da medula óssea (GCM e o que recebeu células mononucleares autólogas com proteína morfogenética óssea (GCM+BMP. Após exposição do sulco troclear da articulação fêmoro-tíbio-patelar esquerda removeu-se um segmento em forma de cunha. O local foi preenchido com enxerto osteocondral alógeno conservado em glicerina a 98%. No GC foi realizada a enxertia óssea, no GCM além do enxerto foi inoculado 2x106 células mononucleares e no GCM+BMP foi administrada intra-articularmente células associadas á 1 μg de proteína morfogenética óssea. A resposta osteoblástica foi mensurada por meio da análise sérica de fosfatase alcalina no dia 0 (pré-operatório 3, 15, 30, 45 após cirurgia, além do acompanhamento por exames radiográficos. Procedeu-se a análise de variância em blocos ao acaso, esquema fatorial e teste de Tukey (p=0,05. A média geral do GCM foi superior quando comparada aos outros grupos e as maiores taxas estiveram entre o 15° e 45° dias de pós-operatório. A discrepância dos valores entre os indivíduos do mesmo grupo proporcionam dúvidas na veracidade do teste. Palavras chave: Células-tronco, Consolidação de fraturas, Marcador ósseo

Introducción a los antisépticos

Directory of Open Access Journals (Sweden)

Laura López González

2014-05-01

Full Text Available Los antisépticos son agentes antiinfecciosos de uso local sobre piel o mucosas, lo cual los distingue de los desinfectantes, que se usan sobre superficies inanimadas, debido normalmente a su toxicidad. En este capítulo explicamos las diferencias entre los múltiples posibles antisépticos, prestando especial atención a los más comunes como el alcohol, la clorhexidina, la povidona yodada y el agua oxigenada. Finalmente hacemos hincapié en las diferentes formulaciones de los antisépticos, que los hacen más útiles para indicaciones determinadas.

Efeito da saturação por bases sobre a atividade de fosfatases em um solo de tabuleiro costeiro cultivado com citros. I. Correlações entre a atividade enzimática e as diferentes características do solo alteradas pela calagem

Directory of Open Access Journals (Sweden)

M. F. Fernandes

1998-09-01

Full Text Available Neste trabalho, utilizaram-se amostras de um Podzol cultivado com citros, na Estação Experimental de Umbaúba, Umbaúba-SE (Centro de Pesquisa Agropecuária dos Tabuleiros Costeiros-EMBRAPA, para avaliar a atividade de fosfatases ácidas e alcalinas em solo de tabuleiro costeiro submetido a diferentes porcentagens de saturação por base (32, 45, 68 e 76,6%, bem como as relações entre essas atividades e algumas características do solo. Respostas positivas ao incremento da saturação por bases foram observadas para pH, teor de matéria orgânica, fósforo disponível e atividade de fosfatases ácidas e alcalinas, em seus respectivos valores de pH ótimo de 6,5 e 11,0, ao passo que a biomassa microbiana não foi alterada significativamente por esse fator. Observou-se que a atividade da fosfatase ácida apresentou correlação significativa e positiva com a matéria orgânica e teores de cálcio e de magnésio trocáveis. A resposta das fosfatases alcalinas à saturação foi influenciada pelo pH, pela matéria orgânica, pelos teores de cálcio e de magnésio e pela biomassa microbiana do solo. Nenhuma das duas enzimas apresentou correlação com os teores de fósforo encontrados no solo analisado. No entanto, observou-se inibição da atividade das enzimas, quando a análise foi realizada em uma solução de reação com concentração de 124 ∝g mL-1 de P.

Efecto de la atorvastatina en el tratamiento de la periodontitis crónica moderada

Directory of Open Access Journals (Sweden)

Miriam Rocha Navarro

2008-01-01

Full Text Available Introducción: Las estatinas son inhibidores de la hidroximetil-glutaril Coenzima A reductasa que han sido empleadas para el tratamiento de la hipercolesterolemia. Se ha reportado que tienen efectos favorables sobre el metabolismo óseo disminuyendo el riesgo de fracturas y en algunos estudios un aumento de la densidad mineral ósea. Por lo anterior, el objetivo de este estudio fue comparar el tratamiento periodontal más atorvastatina (ATV sobre los parámetros periodontales y hueso alveolar versus tratamiento periodontal más placebo en pacientes con periodontitis crónica moderada (PCM. Materiales y Métodos: Estudio controlado, doble ciego, aleatorizado, en el que participaron 38 pacientes con PCM de 35 a 65 años, a los cuales se les administró por tres meses un tratamiento con ATV (20 mg/día, n=19 o complejo B (grupo control n=19. Todos los pacientes recibieron el mismo tratamiento periodontal al inicio del estudio. Se tomaron muestras de sangre en ayuno para cuantificar glucosa, perfil de lípidos, fosfatasa alcalina, N-telopéptidos y 17-estradiol. Se evaluaron los siguientes parámetros periodontales: Profundidad de las bolsas periodontales, sangrado gingival, recesión gingival, pérdida de inserción, movilidad dental, y placa dentobacteriana, además de la distancia de la cresta ósea alveolar a la unión cemento esmalte (COA-UCE. Todas las mediciones se realizaron al inicio y al final del estudio. Para el análisis de los datos se utilizó la prueba t para muestras dependientes y su alternativa no paramétrica para observar diferencias entre los dos grupos. La corrección de Bonferroni para comparaciones múltiples fue calculada en base a los valores de P. Para observar diferencias entre los grupos por género y estado menopáusico se utilizó la prueba t para muestras independientes o su alternativa no paramétrica U de Mann-Whitney. Se consideró una p< 0.05 como significativa. Resultados: Los parámetros periodontales mejoraron

Development of a lyophilized formulation for preparing the radiopharmaceutical {sup 177}Lu-DOTA-Anti-CD20; Desarrollo de una formulacion liofilizada para la preparacion del radiofarmaco {sup 177}-DOTA-Anti-CD20

Energy Technology Data Exchange (ETDEWEB)

Serrano E, L. A.

2015-07-01

The radiolabeled proteins are molecules of interest in nuclear medicine for their diagnostic and therapeutic application in cancer. Antibodies, such as chimeric monoclonal antibody Anti-CD20 rituximab, have established themselves as suitable vectors of radionuclides (e.g. {sup 177}Lu) , introducing high affinity by the surface antigens over- expressed and widely distributed in cells involved in certain diseases. The aim of this work was to design, optimize and document the production process of radiopharmaceutical {sup 177}Lu-DOTA-Anti-CD20 for sanitary registration request to the Comision Federal para la Proteccion contra Riesgos Sanitarios (COFEPRIS). First, a raw material analysis using the Ft-Mir technique and gamma spectrometry was performed. Then, was carried out the development of the lyophilized formulation for the preparation of {sup 177}Lu-DOTA-Anti-CD20, in which an ANOVA was performed where the dependent variable was the radiochemical purity. The optimal pharmaceutical formulation was: 5 mg DOTA-CD20 and 80 mg Mannitol to be reconstituted with 1 m L of acetate buffer 0.25 M, ph 7, with an incubation time of 15 min at 37 degrees Celsius in a dry bath. Once completed the development of the lyophilized formulation, we proceeded to the optimization of the production process, development and validation of the analytical method. Three batches were prepared under protocols of Good Manufacturing Practice, which met pre-established specifications as sterile and endotoxin-free of bacterial formulations, with greater that 95% of radiochemical purity. Currently, is conducting the study of shelf stability. Upon completion of the stability studies, the legal record of {sup 177}Lu-DOTA-Anti-CD20 will be integrated with documented evidence of the quality and stability of the formulation of this radiopharmaceutical. (Author)

IgA anti-Streptococcus mutans em crianças com e sem cárie dentária Anti-Streptococcus mutans IgA in children with and without dental caries

Directory of Open Access Journals (Sweden)

Suzete Cristina YAZAKI

1999-07-01

Full Text Available A cárie dentária é uma doença infecciosa crônica que necessita pelo menos quatro componentes para desenvolver-se: hospedeiro suscetível, microbiota patogênica, dieta rica em sacarose e tempo. Este trabalho estuda as correlações existentes entre estreptococos salivares do grupo mutans, placa bacteriana e anticorpos IgA anti-Streptococcus mutans em crianças com e sem experiência de cárie. Para tanto, utilizou-se o meio Mitis Salivarius (DIFCO para determinar o número de Unidades Formadoras de Colônias (UFC/ml, o Índice de Higiene Oral Simplificado (IHOS para mensurar a quantidade de placa bacteriana e a técnica ELISA para detectar anticorpos anti-S. mutans. Os resultados obtidos mostraram que não existe uma correlação entre os níveis salivares de estreptococos (UFC/ml e IgA anti-S. mutans na população estudada. No grupo com cárie, uma correlação positiva, estatisticamente significante, foi observada entre o Índice de placa e IgA específica.Dental caries is a chronic infectious disease that needs at least four components to develop. As a susceptible host, a pathogenic microbiota, a high-sucrose diet and time. This work assess the relationships between Streptococcus mutans and dental plaque; Streptococcus mutans and IgA antibodies in children with and without caries experience. In order to achieve this goal we have used Mitis Salivarius bacitracin agar (DIFCO to determine the colony forming units (CFU/mL, the simplified oral hygiene index (SOHI for measuring bacterial plaque and ELISA for antibody detection. The results obtained have not shown any correlations between colony forming units of S. mutans and IgA antibodies. A significant correlation was found between bacterial plaque index and specific IgA in children with carious lesions.

Penyekat Beta sebagai Terapi Anti-Remodeling pada Gagal Jantung

Directory of Open Access Journals (Sweden)

Hilman Zulkifli Amin

2015-09-01

now, studies and researches related to that topic are still beingconducted. This paper made to acknowledge beta-blocker as anti-remodeling therapy in heartfailure. Structured PubMed search was conducted and yielded 93 articles; whereafter the inclusionand exclusion criteria were applied, 25 articles remained. After reading the full texts, 11 articleswere appraised concerning its validity, relevance, and aplicability. From 11 articles appraised, it was known that beta-blocker could be acted as anti-remodeling therapy by improving the heart function as shown by the increase of EF and decrease of LVESV and LVEDV in heart failurepatients on those studies. Keywords: Beta-blocker, Anti-remodelling, heart failure /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}

Relação tireóide-gônadas e níveis plasmáticos de fósforo, cálcio e fosfatase alcalina em ratas Relationship between thyroid, gonads and plasmatic levels of phosphorus, calcium and alkaline phosphatase in rats

Directory of Open Access Journals (Sweden)

R. Serakides

2000-12-01

Full Text Available A relação tireóide-gônadas-metabolismo ósseo foi estudada em ratas Wistar adultas, castradas ou intactas e mantidas em estado hipertireóideo ou eutireóideo por períodos de 30, 60 e 90 dias. Foram utilizadas como características do metabolismo ósseo o cálcio, o fósforo e a atividade da fosfatase alcalina plasmáticos, correlacionando-os com os valores de estrógeno, de progesterona e de T4 livre. Verificou-se que o hipogonadismo e o hipertireoidismo alteram as características plasmáticas do metabolismo ósseo. O hipertireoidismo induz hiperfosfatemia e hipocalcemia, o hipogonadismo tem pouca influência sobre o fósforo, mas potencializa a hiperfosfatemia e a hipocalcemia desencadeadas pelo hipertireoidismo. Com relação à fosfatase alcalina, conclui-se que o hipertireoidismo reduz o efeito do hipogonadismo sobre a atividade dessa enzima.The interrelation between thyroid, gonads and osseous metabolism was studied in either intact or castrated adult female rats kept under hyperthyroidism or euthyroidism for 30, 60, or 90 days. Plasmatic levels of phosphorus, calcium, and alkaline phosphatase were measured to assess the osseous metabolism. These characteristics were correlated to the levels of estrogen, progesterone, and free T4. Either hypogonadism or hyperthyroidism interfered with the plasmatic characteristics of osseous metabolism. Hyperphosphatemia and hypocalcemia were induced by hyperthyroidism, whereas the hypogonadism had little effect on the levels of phosphorus, but it had a potencialization effect on the hyperphosphatemia and hypocalcemia induced by hyperthyroidism. The effect of hypogonadism on the alkaline phosphatase activity was reduced by the hyperthyroidism.

Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix Association between anti-Ras and anti-HPV16 E4/E7 antibodies with cervical intraepithelial lesions

Directory of Open Access Journals (Sweden)

Sara Vázquez-Corzo

2003-10-01

Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.OBJECTIVE: To evaluate whether serum antibodies anti-E4, E7 and Ras could be used as markers for early cervical lesions associated with HPV (human papillomavirus. MATERIAL AND METHODS: A seroepidemiological case-control study was conducted between March 1999 and April 2000 at the dysplasia clinic of Hospital General Doctor Gea Gonzalez, in Mexico City, to evaluate the presence of antibodies anti-E4, E7, and Ras through a sandwich ELISA. Analysis was done using odds ratios and 95% confidence intervals. RESULTS: Anti-E7 antibodies were associated to women with CIN III lesions, while anti-E4 and Ras antibodies were strongly associated with CIN I-II lesions. The antibody profile of women with different

Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix

Directory of Open Access Journals (Sweden)

Vázquez-Corzo Sara

2003-01-01

Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.

Propuesta de un programa para la\teducación anti-doping en las escuelas comunitarias de deportes del municipio Pinar del Río

Directory of Open Access Journals (Sweden)

Ida Galván Rodríguez

2009-12-01

Full Text Available Dada la magnitud que ha cobrado el dopaje en el mundo actual, este sigue siendo un gran problema esencial del deporte moderno a pesar de todas las acciones realizadas, políticas de control y sanciones. Por tal motivo, esta situación debe ser reevaluada desde otras alternativas como la que proponemos en este trabajo. Planteamos un proyecto de Educación Anti-doping para las Escuelas Comunitarias de Deportes en el municipio Pinar del Río pues estas constituyen la institución social encargada de desarrollar funciones educativas de Deporte, Educación Física, Recreación Física y Cultura Física en general, en su territorio. El mismo plantea una serie de objetivos, acciones y actividades, abordadas fundamentalmente en las vertientes extracurricular no formal y extracurricular informal, el cual se respalda en todo un conjunto de consideraciones científico-metodológicas. Finalmente, planteamos útiles recomendaciones que ayuden a generalizar esta alternativa educativa y a profundizar en cómo enriquecer una educación Anti- doping desde la comunidad.

Comparison of semi-automatized assays for anti-T. gondii IgG detection in low-reactivity serum samples: importance of the results in patient counseling Comparação de ensaios semi-automatizados para pesquisa de IgG anti-T. gondii em amostras de soros de baixa reatividade: importância dos resultados no aconselhamento do paciente

Directory of Open Access Journals (Sweden)

Paulo Guilherme Leser

2003-06-01

Full Text Available Toxoplasmosis is a disease which can cause severe congenital infection and is normally diagnosed by the detection of T. gondii specific antibodies in the serum of infected patients. Several different tests allow to distinguish recent from past infections and to quantify anti-T. gondii specific IgG, and the results can be used as markers for immunity. In the present study, we compare the performance of two different methodologies, the Elfa (bioMérieux S.A and the Meia (Abbott Laboratories in detecting T. gondii specific IgG in low-reactivity sera. Of 76 analyzed samples, three presented discrepant results, being positive in the Abbott AxSYM Toxo IgG assay, and negative in the bioMérieux Vidas Toxo IgG II assay. By using other tests, the three sera were confirmed to be negative. The results are discussed in the context of their importance for patient management, especially during pregnancy.Toxoplasmose, doença conhecida por sua severidade na infecção congênita é geralmente diagnosticada pela demonstração de anticorpos específicos contra antígenos de T. gondii, presentes no soro de indivíduos infectados. Diferentes testes são disponíveis para diferenciar infecção recente de infecção pregressa, para quantificar anticorpos IgG anti-T. gondii nos soros dos pacientes e utilizar os resultados como marcadores de imunidade. Neste trabalho apresentamos os resultados do estudo comparativo de duas tecnologias, Elfa (bioMérieux S.A. e Meia (Abbott Laboratories, para pesquisa de anticorpos IgG anti-T. gondii em amostras de soros de baixa reatividade. De 76 amostras processadas, três apresentaram resultados discrepantes, reagentes para AxSYM Toxo IgG e não-reagentes para Vidas Toxo IgG II. A confirmação dos resultados, feita por bateria de testes, mostrou que todas as três amostras eram não-reagentes. Os resultados são discutidos em sua importância e orientação clínica, principalmente para a paciente gestante.

RAY TRACING RENDER MENGGUNAKAN FRAGMENT ANTI ALIASING

Directory of Open Access Journals (Sweden)

Febriliyan Samopa

2008-07-01

Full Text Available Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Rendering is generating surface and three-dimensional effects on an object displayed on a monitor screen. Ray tracing as a rendering method that traces ray for each image pixel has a drawback, that is, aliasing (jaggies effect. There are some methods for executing anti aliasing. One of those methods is OGSS (Ordered Grid Super Sampling. OGSS is able to perform aliasing well. However, this method requires more computation time since sampling of all pixels in the image will be increased. Fragment Anti Aliasing (FAA is a new alternative method that can cope with the drawback. FAA will check the image when performing rendering to a scene. Jaggies effect is only happened at curve and gradient object. Therefore, only this part of object that will experience sampling magnification. After this sampling magnification and the pixel values are computed, then downsample is performed to retrieve the original pixel values. Experimental results show that the software can implement ray tracing well in order to form images, and it can implement FAA and OGSS technique to perform anti aliasing. In general, rendering using FAA is faster than using OGSS

Métodos para avaliação das emissões de gases do efeito estufa no sistema solo-atmosfera

Directory of Open Access Journals (Sweden)

Costa Falberni de Souza

2006-01-01

Full Text Available A escolha do método para avaliar as emissões de gases do efeito estufa (GEE é uma etapa importante para o conhecimento e/ou desenvolvimento de práticas agrícolas com potencial de mitigação do aquecimento global. A presente revisão tem por objetivo apresentar vantagens e limitações de métodos utilizados para quantificação dos fluxos de dióxido de carbono (CO2, metano (CH4 e óxido nitroso (N2O no sistema solo-atmosfera. O balanço dos estoques de C orgânico no solo em sistemas conservacionistas de manejo permite avaliar o influxo líquido anual de C-CO2 atmosférico no solo em comparação a sistemas de manejo convencional. Maior sensibilidade na determinação direta das emissões de CO2 in situ pode ser obtida pelo uso de câmaras sobre o solo. Nesse caso, podem ser determinadas taxas diárias com o método da captura do CO2 em solução alcalina e quantificação por titulação, e taxas horárias com o uso de analisadores automáticos de infravermelho ou cromatógrafos a gás. Pelo uso de cromatografia, é possível também a avaliação das emissões de N2O e CH4 os quais apresentam, respectivamente, potencial de aquecimento global 296 e 23 vezes superior ao do CO2. A análise dos três GEE é necessária quando se objetiva avaliar o potencial de um dado sistema de manejo na mitigação do aquecimento global, o qual pode ser expresso em C equivalente.

Normas anti-elusivas domésticas e internacionais no direito tributário internacional

OpenAIRE

Okuma, Alessandra de Souza

2009-01-01

O objeto deste trabalho é a análise das normas anti-elusivas veiculadas por leis brasileiras e pelas Convenções Internacionais para evitar a dupla tributação ( CIT ), no que concerne ao imposto sobre a renda da pessoa jurídica. Partiremos da teoria dos sistemas de Luhmann, dos princípios constitucionais tributários e das normas de direito privado para construir a norma geral anti-elusiva doméstica, tal como delineada pelo art. 116, parágrafo único do CTN. Demonstraremos que a aplicação dessa ...

Laparoscopic treatment of type III para-oesophageal hernia | Van ...

African Journals Online (AJOL)

Type III congenital para-oesophageal hernia is a rare condition in children and is ... portion of the stomach and the gastro-oesophageal junction into the chest. ... in the hands of paediatric surgeons familiar with laparoscopic anti-reflux surgery.

Utilización de indicadores metabólicos en la valoración de la transferencia de inmunidad pasiva en neonatos bovinos Assessment of the passive transference of immunity in calves through metabolic indicators

Directory of Open Access Journals (Sweden)

Angie Fairut Carrillo

2009-07-01

Full Text Available Resumen Para determinar la utilidad de la actividad sérica de la fosfatasa alcalina (FA y las proteínas séricas totales (PST como indicadores de transferencia de inmunidad en terneros por el consumo de calostro en sus primeros días de vida, en un hato del trópico bajo de Colombia se escogieron al azar 15 vacas de las razas Gyr, Brahaman y el cruce Gyr x Holstein y sus respectivos terneros. En las vacas se recolectaron aproximadamente 300 ml de calostro el primer día del parto para determinar sólidos totales, proteína total y cenizas. En los terneros se tomaron muestras de sangre sin anticoagulante mediante venipunción yugular los días 0, 4, 12 y 30 posnacimiento. La concentración de las PST se determinó por refractometría y la FA mediante colorimetría enzimática. Se encontró relación alta y significativa (P Fifteen bovines and their calves were chosen randomly in order to determine the usefulness of the Alkaline phosphatase's (ALP seric activity and the total seric proteins (TSP as indicators of transference of immunity in calves through colostrums' consumption in their first days of life. Three hundred ml (300 ml of colostrums of the first day after delivery were collected from the cows. In it total solids, crude protein and ashes were found. Blood samples without anticoagulant and through jugular venipuncture were taken from the calves on the 0, 4, 12, and 30th day after delivery. The concentration of the TSP was determined through refractometry, while the ALP was determined through the enzymatic colourimetric assay. A high and statistically significant relationship between the percentages of crude protein of the colostrums and the total solids was found, thus, it can be concluded that just one measurement is valid in order to study both indicators. The effect of time on the concentrations of TSP as well as on the enzymatic activity was evaluated through the use of ANOVA (Analysis of Variance. In the TSP there was a

Análisis de plásmidos recombinantes con insertos de ADN genómico de Plasmodium falciparum

Directory of Open Access Journals (Sweden)

Fernando Ángel S.

1988-06-01

Full Text Available Se describe la preparación de plásmidos pUC18 recombinantes provenientes de la construcción de una biblioteca genómica del parásito Plasmodium falciparum usando una modificación del método de lisis alcalina (1 . La cantidad de plásmido producido es suficiente para llevar a cabo varios análisis de digestión con enzimas de restricción y electroforesis en geles de agarosa.

First report of anti-TIF1γ dermatomyositis in a patient with myelodysplastic syndrome

Directory of Open Access Journals (Sweden)

B. Palterer

2017-08-01

Full Text Available Inflammatory myopathies as para-neoplastic phenomena were first described by Sterz in 1916. Recently, myositis specific autoantibodies were described in cancer-associated myositis. Anti-transcription intermediary factor 1 gamma (anti-TIF1γ antibodies have been found in both young adults affected by juvenile dermatomyositis and in elderly patients with cancer-associated myositis. In this regard, we report herein the first case of anti-TIF1γ dermatomyositis secondary to a myelodysplastic syndrome.

Occurrence of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies in goats and sheep in western Maranhão, Brazil Ocorrência de anticorpos IgG anti-Neospora caninum e anti-Toxoplasma gondii em caprinos e ovinos do oeste do Maranhão, Brasil

Directory of Open Access Journals (Sweden)

Larissa Martins de Brito Moraes

2011-12-01

Full Text Available Neosporosis and toxoplasmosis are parasitic diseases which can cause reproductive problems in goats and sheep. The current study aimed to determine the occurrence of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies in goats and sheep from the districts of Amarante do Maranhão and Buritirana, Imperatriz microregion, western area of Maranhão State, northeastern Brazil, and to assess factors associated to infection by these etiologic agents. Blood samples from 110 animals (46 goats and 64 sheep from five herds were collected, and indirect immunofluorescence assay was used for serological testing. Of 46 goat samples, 17.39% (n = 8 showed anti-N. caninum antibodies and 4.35% (n = 2 anti-T. gondii, while of 64 sheep samples 4.69% (n = 3 and 18.75% (n = 12 showed anti-N. caninum and anti-T. gondii antibodies, respectively. No significant difference regarding the presence of domestic cats and/or dogs on the property and veterinary care was seen for both etiologic agents studied. However, food supplementation and animal reproductive failure were significantly (p A neosporose e a toxoplasmose são doenças parasitárias que podem causar problemas reprodutivos em caprinos e ovinos. O objetivo deste estudo foi determinar a ocorrência de anticorpos IgG anti-Neospora caninum e anti-Toxoplasma gondii em caprinos e ovinos dos municípios de Amarante do Maranhão e Buritirana, microrregião de Imperatriz, Oeste maranhense, Nordeste do Brasil, bem como avaliar fatores associados à infecção por esses agentes etiológicos. Amostras de sangue de 110 animais (46 caprinos e 64 ovinos, provenientes de cinco propriedades, foram coletadas, e a reação de imunofluorescência indireta utilizada para o diagnóstico sorológico. Das 46 amostras de caprinos, 17,39% (n = 8 apresentaram anticorpos anti-N. caninum e 4,35% (n = 2 anti-T. gondii, enquanto das 64 amostras de ovinos, 4,69% (n = 3 e 18,75% (n = 12 apresentaram anticorpos anti-N. caninum e anti

Desarrollo de nuevos materiales de electrodo para la obtención de Hidrógeno a partir de la electrólisis alcalina del agua.

OpenAIRE

Herraiz Cardona, Isaac

2012-01-01

El término "Economía del Hidrógeno" responde a una visión de futuro donde este gas, generado de forma limpia y económica, serviría para alimentar el grueso de las necesidades energéticas de la sociedad. Esta propuesta reduciría la dependencia actual sobre los combustibles fósiles, ya que el hidrógeno podría ser producido a partir de otras fuentes primarias como las renovables o la nuclear. Igualmente se disminuiría la contaminación atmosférica y la emisión de gases de efecto invernadero, pues...

Libertad religiosa, camino para la paz

Directory of Open Access Journals (Sweden)

Antonio Alonso

2011-01-01

Full Text Available La libertad religiosa es el núcleo del Mensaje para la XLIV Jornada Mundial de la Paz que ha dirigido Benedicto XVI. Cuestión, sin duda, extraordinariamente delicada, pues comporta graves implicaciones en materia de seguridad principalmente por dos motivos: primero, porque su violación supone una injusticia difícil de compaginar con el respeto a los Derechos Humanos; segundo, porque dicha libertad no puede ser esgrimida como pretexto para atropellar los derechos de los demás conciudadanos o incluso socavar la propia seguridad del Estado. El Santo Padre denuncia, a su vez, los estragos del relativismo y de los fanatismos, tanto religioso como anti-religioso, y propone el ejercicio del diálogo basado en la razón para superar los obstáculos en la convivencia interpuestos por estas amenazas a la paz y la seguridad.

CONHECIMENTO DE GESTANTES SOBRE O EXAME ANTI-HIV NO PRÉ-NATAL

Directory of Open Access Journals (Sweden)

KARLA DE ABREU PEIXOTO MOREIRA

2006-01-01

Full Text Available La epidemia de SIDA afecta cada vez más a las mujeres en edad fértil. La realización del examen anti-VIH durante el prenatal es una importante estrategia para reducir la morbio mortalidad por esta causa. El objetivo planteado fue el de investigar el nivel de conocimiento de las embarazadas cuanto a la importancia de la realización del examen anti-VIH en el prenatal. Estudio descriptivo con 50 embarazadas mayores de 18 años, que realizaban prenatal de bajo riesgo en consulta subsiguiente sin diagnóstico firmado para SIDA. La edad de las embarazadas predominó entre 20 a 25 años; la mayoría había sido abordada en consultas anteriores sobre la importancia del examen anti-VIH y un 92% confirmó que había realizado ese examen después de la primera consulta de prenatal. Se reafirman los beneficios de este examen en el prenatal y la importancia de la educación en la salud durante la prevención y el control de la enfermedad.

Neurobiologia do transtorno de personalidade anti-social Neurobiology of anti-social personality disorder

Directory of Open Access Journals (Sweden)

Cristina Marta Del-Ben

2005-01-01

Full Text Available Nos últimos anos, tem havido um interesse crescente a respeito de uma melhor compreensão sobre o comportamento anti-social. O aumento da criminalidade e violência urbanas pode ter contribuído para esse maior interesse. Além de fatores psicossociais, outros biológicos têm sido implicados na fisiopatogenia do transtorno de personalidade anti-social (TPAS. Estudos de neuroimagem apontam o envolvimento de estruturas cerebrais frontais, especialmente o córtex orbitofrontal, e a amígdala. Também tem sido sugerido que prejuízos na função serotonérgica estariam associados à ocorrência de comportamento anti-social, já que pacientes com diagnóstico de TPAS apresentam respostas hormonais atenuadas a desafios farmacológicos com drogas que aumentam a função serotonérgica cerebral e redução da concentração de receptores serotonérgicos. Uma abordagem ampla dos diferentes fatores possivelmente envolvidos na fisiopatogenia do TPAS poderia contribuir para o desenvolvimento de novas técnicas de prevenção e intervenção.Violence and crime have been increasing considerably in urban societies. As a consequence, some efforts have been made aiming at a better understanding of antisocial bevaviour. Apart from psychosocial factors, some evidences suggest the occurrence of biological factors in the pathogenesis of antisocial personality disorders (ASPD. Neuroimaging studies have shown the involvement of prefrontal areas, especially orbitofrontal cortex, and amygdala. Also, impaired serotonin (5-HT neurotransmission has been implicated, since patients with ASPD present alterations in measures of 5-Ht system, such as blunted hormonal response to 5-HT pharmacological challenges and reduced 5-HT receptors numbers. A comprehensive approach of antisocial behavior, including biological and psychosocial aspects could lead to the development of new techniques for prevention and intervention in ASPD.

Queratinasas microbianas: microorganismos, producción y caracterización

OpenAIRE

Cavello, Ivana Alejandra

2013-01-01

Actualmente las proteasas alcalinas son ampliamente utilizadas en la industria del cuero, en distintas formulaciones de detergentes, también en el proceso de recuperación de plata, y en la producción de hidrolizados de proteínas. Comúnmente, todas estas proteasas se obtienen de fuentes microbianas que crecen en sustrato relativamente caros. Muchos estudios demuestran que cerca del 40% del costo de producción de estas enzimas está relacionada con la composición del medio de cultivo. Para reduc...

Veneno para la mente: La participación de los especialistas en salud mental en la campaña anti-cómic estadounidense (1940-1960)

OpenAIRE

Fernández Sarasola, Ignacio

2016-01-01

Entre 1940 y 1960 se desarrolló en Estados Unidos una intensa campaña anti-cómic impulsada por ciudadanos que consideraban que aquellas revistas no solo suponían una mala influencia para sus niños, sino que eran responsables de un incremento en la delincuencia juvenil. Un punto de inflexión se produjo en el momento en que psiquiatras y psicólogos se incorporaron a la citada campaña. Su líder fue Fredric Wertham, un psiquiatra de origen germano que acusó a los cómics de dañar la mente de los n...

Modelo matemático para estructura tubular de edifico antisísmico

Directory of Open Access Journals (Sweden)

Mazzeo, Agustin

1972-02-01

Full Text Available This mathematical model was worked out when seeking a structural pattern for a building with a main steel structure, apt for the seismic circumstances of the city of Caracas. Of the various possibilities that were considered, it was decided the one which best met the conditions of the project involved the use of the so called external flexible tube, to withstand horizontal thrusts. The analysis of the space properties of the perimetral tube, through the use of a plane image of the structure, constitutes the mathematical model described in this article.El modelo matemático objeto del artículo fue desarrollado al estudiar la solución estructural para un edificio con estructura principal de acero, proyectado para las condiciones sísmicas de la ciudad de Caracas. De las varias alternativas analizadas, se concluyó que la que mejor permitía cumplir con las exigencias del proyecto consistía en utilizar como solución, para resistir los efectos horizontales, el sistema estructural conocido como tubo exterior flexible. El análisis de las características espaciales del tubo perimetral mediante el uso de una imagen plana de la estructura, constituye el modelo matemático que se describe en el artículo.

Importancia del escrutinio para tuberculosis previo a la administración de agentes anti-TNF-α en uveítis: a propósito de un caso clínico

Directory of Open Access Journals (Sweden)

Enrique Leopoldo Zaldívar-Orta

2014-10-01

Conclusión: Es indispensable realizar pruebas de escrutinio para tuberculosis latente antes de iniciar tratamiento con agentes biológicos anti-TNF-α, particularmente con infliximab. Las pruebas que miden la producción de interferón gamma (IFN-γ in vitro en respuesta a los antígenos tuberculosos son una alternativa a la prueba cutánea clásica con tuberculina (PPD en la detección de tuberculosis, particularmente en individuos vacunados con el bacilo de Calmette-Guérin (BCG.

Detecção de anticorpos anti-Toxoplasma gondii em suínos criados e abatidos no Estado da Bahia, Brasil Detection anti-Toxoplasma gondii antibodies in swines bred and abated in the Bahia State, Brazil

Directory of Open Access Journals (Sweden)

Rodrigo A. Bezerra

2009-09-01

Full Text Available Objetivou-se verificar a ocorrência de anticorpos anti-Toxoplasma gondii em suínos criados e abatidos no Estado da Bahia. Foram coletadas e examinadas 465 amostras de sangue de suínos provenientes de criações de diferentes locais desse estado. Para a pesquisa de anticorpos anti-T. gondii, foi utilizada a técnica de Imunoadsorção Enzimática (ELISA e considerados positivos todos os animais com títulos iguais ou maiores que 1:16. Desses, 18,27% (85/465 foram positivos para anticorpos anti-T. gondii, sendo 30,76% (24/78 em Ilhéus, 18,10% (21/116 em Itabuna e 14,76% (40/271 em Simões Filho. Foram observadas diferenças significativas quanto ao sexo dos animais (p = 0,0171, ao sistema de criação (p = 0,0002 e à procedência dos animais (p = 0,0278 no município de Itabuna. Anticorpos anti-T. gondii foram encontrados nos animais estudados, podendo ser estes animais fonte de infecção para a população humana local.This study was performed to verify the occurrence of anti-Toxoplasma gondii antibodies in swine raised and slaughtered in the state of Bahia, Brazil. Four hundred sixty five swine blood samples from farms of different cities had been collected and examined. Anti-T. gondii antibodies was detected by the enzyme-linked immunosorbent assay (ELISA and considered positive all the animals with equal or bigger headings than 1:16. From these, 18.27% (85/465 of total sample were positive for T. gondii, 30.76% (24 in Ilhéus, 18.10% (21/116 in Itabuna and 14.76% (40/271 in Simões Filho. Significant differences were observed regarding animal sex (p = 0.0171, raising system (p = 0.0002 and origin of the animals (p = 0.0278 in the city of Itabuna. The occurrence of anti-T. gondii antibodies shows that swine can be a source of infection for the local human population.

Relación entre la solubilidad en álcali y los parámetros de tracción de un hilado de estambre sometido a tratamientos ácidos y oxidantes

OpenAIRE

Gacén Guillén, Joaquín; Naik Kardile, Arun; Cayuela Marín, Diana; Palet Alsina, Daniel

2003-01-01

Un hilado de lana peinada ha sido sometido a tratamientos ácidos y con peróxido de hidrógeno a efectos de preparar sustratos que presentan un amplio intervalo de solubilidades alcalinas. Se han relacionado los parámetros de tracción con la solubilidad alcalina de los correspondientes sustratos. Este estudio ha permitido ampliar la información sobre el motivo del aumento de la solubilidad en álcali producido por el blanqueo con peróxido de hidrógeno. Se ha relacionado la solubilidad alcalina d...

Hepatites pós-transfusionais na cidade de Campinas, SP, Brasil: II. Presença dos anticorpos anti-HBc e anti-HCV em candidatos a doadores de sangue e ocorrência de hepatites pós-transfusionais pelo vírus C nos receptores de sangue ou derivados Post-transfusional hepatitis in the city of Campinas, SP, Brazil: II- Presence of anti-HBc and anti-HCV antibodies in blood donors and occurrence of post-transfusional hepatitis C virus in recipients of blood or derivates

Directory of Open Access Journals (Sweden)

Fernando Lopes Gonçales Júnior

1993-02-01

Full Text Available Pesquisamos os anticorpos anti-HBc e anti-HCV em amostras de soros provenientes de 799 candidatos a doadores, que tiveram suas unidades de sangue ou derivados transfundidas a 111 receptores. O anti-HBc e o anti-HCV foram reagentes, em respectivamente 9 e 2,1% dos doadores testados. Observamos que entre os 111 receptores, 44 haviam recebido pelo menos uma unidade anti-HBc positiva e 67 haviam sido transfundidos somente com unidades anti-HBc negativas. Houve um risco 4,5 vezes maior de aquisição de hepatite por vírus C pelos receptores que receberam pelo menos uma unidade anti-HBc positiva Se a pesquisa do anti-HBc fosse realizada na triagem sorológica dos doadores de sangue, cerca de 56% dos casos de HVC nos receptores saiam evitados. A população de receptores que recebeu pelo menos uma unidade anti-HCV reagente, apresentou um risco 29 vezes maior de adquirir esta hepatite, quando comparada aos receptores transfundidos com todas as unidades anti-HCV negativas. A realização do teste para a pesquisa do anti-HCV na triagem dos doadores de sangue, preveniria 79% dos casos de HVC pós-transfusionais. Os candidatos a doadores brasileiros parecem ser acometidos simultânea ou sequencialmente, pelos vírus B e C das hepatites, pois, 44,4% dos doadores anti-HCV positivos, também foram anti-HBc positivos. A realização dos testes para as pesquisas dos anticorpos anti-HBc e anti-HCV, nas triagens hemoterápicas, está indicada para prevenir a transmissão de hepatites pós-transfusionais, em nosso meio.We have analysed anti-HBc and anti-HCV antibodies in serum samples from 799 donors which had their blood or derivates transfused to 111 recipients. Anti-HBc and anti-HCV were reactive in respectively 9 and 2.1% of the donors tested. We have observed that among the 111 recipients, 44 had received at least one positive anti-HBc unit and 67 had been transfused only with negative anti-HBc, units. The risk of developing hepatitis C virus was 4.5 times

MONOTERPENOS COM ATIVIDADE ANTI-INFLAMATÓRIA: UMA PROSPECÇÃO TECNOLÓGICA

Directory of Open Access Journals (Sweden)

Lucindo José Quintans-Júnior

2014-09-01

Full Text Available A utilização de plantas medicinais para o tratamento de doenças tornou-se popular em todo planeta. Nesse sentido, a busca por moléculas com efetividade para condições inflamatórias é uma alternativa para o manejo farmacológico. Dentro deste contexto estão inseridos os monoterpenos, constituintes majoritários de óleos essências de espécies vegetais aromáticas dotados de diversas atividades farmacológicas, inclusive propriedades anti-inflamatórias. Desta forma, objetivo desse trabalho foi realizar um rastreamento dos depósitos de patentes sobre o efeito anti-inflamatório de monoterpenos até o momento. Para isso, a prospecção foi realizada no Banco Europeu de Patentes, no banco da Organização Mundial de Propriedade Intelectual, no Banco Americano de Marcas e Patentes e no banco de dados do Instituto Nacional de Propriedade Industrial do Brasil. O maior número de pedidos de patentes encontrados foi no banco da Organização Mundial de Propriedade Intelectual. Os maiores depositantes foram os Estados Unidos e a Organização Mundial de Patentes. A classificação internacional mais abundante nessa prospecção foi a A61K.

CONHECIMENTO DE GESTANTES SOBRE O EXAME ANTI-HIV NO PRÉ-NATAL

OpenAIRE

KARLA DE ABREU PEIXOTO MOREIRA; LÍGIA DE OLIVEIRA BRAGA; ÂNGELA MARIA LEAL DE MORAES VIEIRA; MARIA VERACI OLIVEIRA QUEIROZ

2006-01-01

La epidemia de SIDA afecta cada vez más a las mujeres en edad fértil. La realización del examen anti-VIH durante el prenatal es una importante estrategia para reducir la morbio mortalidad por esta causa. El objetivo planteado fue el de investigar el nivel de conocimiento de las embarazadas cuanto a la importancia de la realización del examen anti-VIH en el prenatal. Estudio descriptivo con 50 embarazadas mayores de 18 años, que realizaban prenatal de bajo riesgo en consulta subsiguiente sin d...

Tectônica transcorrente mesozoica-cenozoica na Borda Leste da Bacia do Paraná, Estado de Santa Catarina

OpenAIRE

Patricia Duringer Jacques

2013-01-01

O presente estudo teve como objetivo principal avaliar as estruturas rúpteis da borda leste da Bacia do Paraná em Santa Catarina, a partir do estudo dos lineamentos estruturais que afetaram as rochas do embasamento, da sucessão ondwânica, das vulcânicas da Formação Serra Geral e das alcalinas associadas ao Domo de Lages e ao Complexo Carbonatítico de Anitápolis. Para atingir estes objetivos, foram efetuados levantamentos de campo de análise estrutural dos lineamentos, integração do arca...

Integrated dynamic model of the alkaline delignification process of lignocellulosic biomass

OpenAIRE

FUERTEZ, JOHN; RUIZ, ANGELA; ALVAREZ, HERNÁN; MOLINA, ALEJANDRO

2012-01-01

A pesar que en la literatura existen varios estudios que describen modelos de deslignificación alcalina, estos fueron desarrollados originalmente para la industria de papel, y no incluyen los efectos de las variables de operación importantes tales como temperatura, concentración del hidróxido, relación sólido-líquido, tamaño de partícula, composición de la biomasa (fracciones de hemicelulosa y lignina) y mezclado. Esta carencia de modelos detallados de las etapas del pretratamiento incitó el ...

Analgesia regional periférica com lidocaína em paciente queimado: relato de caso

OpenAIRE

Geier, Karl Otto

2004-01-01

JUSTIFICATIVA E OBJETIVOS: A realização de anestesia regional em pacientes queimados é dificultosa pela localização aleatória das lesões térmicas. Elevadas taxas de alfa1 glicoproteína ácida com grande afinidade para drogas alcalinas, especialmente a lidocaína, têm sido observadas nesses pacientes. Este caso relata como o uso intermitente de anestesia e analgesia regional periférica com altas doses de lidocaína podem ser úteis em fornecer efetiva analgesia num paciente com queimaduras de segu...

ESTUDO BIOQUÍMICO DO POTENCIAL DA MEMBRANA AMNIÓTICA NA FIBROSE HEPÁTICA INDUZIDA EM RATOS

Directory of Open Access Journals (Sweden)

Shaine Silva Maciel

2016-11-01

Full Text Available A fibrose hepática causada por obstrução do ducto biliar induz alterações tanto na estrutura como na função do fígado, e pode levar a cirrose e insuficiência hepática, quando não tradada. A membrana amniótica humana (MA devido às suas propriedades anti-inflamatórias e anti-fibróticas pode ser uma terapia alternativa. A proposta desse trabalho é analisar as alterações nos níveis séricos das principais enzimas celulares e da albumina, na fibrose hepática induzida pela ligadura do ducto biliar (LDB e após o tratamento com a MA. Dez ratos foram divididos nos grupos LDB e LDB+MA, e eutanasiados após 9 semanas da LDB. As amostras de sangue foram processadas bioquimicamente para análise da albumina (ALB, fosfatase alcalina (FA e transaminases (TGP e TGO.  Comparando os grupos experimentais verificou-se que no grupo LDB+MA houve a diminuição da ALB e TGP e no grupo LDB houve a diminuição da FA e TGO, entretanto sem apresentar diferença significante entre os grupos. Conclui-se que a MA, quando aplicada ao fígado no mesmo momento da indução da fibrose, parece não ter exercido efeito significativo na função hepática.

A cluster-randomized controlled trial of handrubs for prevention of infectious diseases among children in Colombia Ensayo controlado aleatorizado por conglomerados sobre el uso de antisépticos para las manos para la prevención de enfermedades infecciosas en los niños en Colombia

Directory of Open Access Journals (Sweden)

Juan C. Correa

2012-06-01

Full Text Available OBJECTIVE: To evaluate the effectiveness of alcohol-based handrubs (ABH in reducing acute diarrheal diseases (ADD and acute respiratory infections (ARI among children 1-5 years of age in childcare centers with limited tap water. METHODS: This was the first cluster-randomized controlled trial in a developing country. The study took place at 42 childcare centers with sporadic and limited water availability in six towns in Colombia. Participants were randomly assigned to use ABH as a complement to handwashing (intervention arm: 21 centers/794 children; or to continue existing handwashing practices (control arm: 21 centers/933 children. ADD and ARI cases were identified through teacher-reported signs and symptoms of illness. Adverse events were monitored. Hazard ratios (HR were obtained using Cox proportional hazards multivariate regression shared frailty models. RESULTS: Child-days of surveillance totaled 336 038. Loss to follow up was 14.5%. For both ADD and ARI, there were no differences in hazard ratios during the first trimester of the study. In the second and third trimesters, significant reductions in the risk of ADD were found in the intervention compared to control arm (HR = 0.55, P OBJETIVO: Evaluar la eficacia del uso de antisépticos para las manos a base de alcohol en la disminución de las enfermedades diarreicas agudas y las infecciones respiratorias agudas en niños de 1 a 5 años de edad en los centros de atención infantil donde el lavado de las manos con agua y jabón no es factible. MÉTODOS. El presente fue el primer ensayo controlado y aleatorizado por conglomerados llevado a cabo en un país en desarrollo. El estudio tuvo lugar en 42 centros de atención infantil con disponibilidad de agua esporádica y limitada ubicados en seis ciudades de Colombia. Se asignó aleatoriamente a los participantes a usar antisépticos a base de alcohol como complemento del lavado de las manos (grupo de intervención: 21 centros/794 niños o a

POBLACIONES MICROBIANAS, ACTIVIDADES ENZIMÁTICAS Y SUBSTANCIAS HÚMICAS EN LA BIOTRANSFORMACIÓN DE RESIDUOS

Directory of Open Access Journals (Sweden)

Roberto Quintero Lizaola

2014-04-01

Full Text Available Se evaluaron poblaciones microbianas, actividades enzimáticas y substancias húmicas (SH durante el compostaje y vermicompostaje (Eisenia andrei Bouché de paja de avena, subproducto de producción de cuerpos fructíferos del hongo comestible Pleurotus ostreatus, con diferentes tamaños: molida (< 2mm y picada (< 4mm. Se muestreó a los 23, 46, 69, 92, 115 y 148 días de la incorporación de lombrices (Eisenia andrei y se comparó con tratamientos sin lombriz. En cada muestreo se midió la actividad enzimática respecto a: el ciclo del carbono (amilasa, celulasa, lipasa e invertasa, ciclo del nitrógeno (proteasa, amidasa, ureasa y nitrogenasa, ciclo del fósforo (fosfatasa ácida y alcalina y ciclo del azufre (arilsulfatasa, y deshidrogenasa. Se evaluaron los ácidos húmicos (AH liofilizados, analizando y determinando, por métodos físicos y químicos, color, carbono y grupos funcionales con espectroscopia infrarroja. Los resultados mostraron la influencia de la lombriz sobre la proporción de la población de bacterias en los tratamientos de paja picada (PP y paja molida (PM tanto en vermicomposta como composta. Los hongos y actinomicetos se presentaron en mayor cantidad en los tratamientos de paja molida con lombriz (PMCL respecto al resto de los tratamientos. El tamaño de paja no tuvo influencia significativa en la proporción de las enzimas en las compostas, a diferencia de los tratamientos con lombriz que tuvieron resultados mayores en las enzimas muestreadas respecto a los tratamientos sin lombriz. Las mayores concentraciones de ácidos húmicos (AH liofilizados se detectaron en el tratamiento de PMCL, así como las mayores concentraciones de enzimas.

Manifestaciones musculo-esqueléticas en pacientes en hemodiálisis crónica

Directory of Open Access Journals (Sweden)

Raúl Alca-Clares

2013-10-01

Full Text Available Objetivos: Determinar la frecuencia y describir las manifestaciones musculoesqueléticas (MME en pacientes en hemodiálisis crónica (HDC. Material y métodos: Es una serie de casos. Se incluyeron 68 pacientes con edad ≥ 18 años, en HDC ≥ 6 meses, del Centro de Hemodiálisis de la Universidad Peruana Cayetano Heredia. Los datos demográficos, clínicos, tiempo en hemodiálisis, dosis de diálisis e ingesta proteica, y calcio, fósforo y fosfatasa alcalina séricos fueron obtenidos de las historias clínicas, en 38 pacientes se determinó PTH sérico y a 37 pacientes se les realizó densitometría ósea como parte de su evaluación. Resultados: La edad promedio fue 52,1 ± 22,1 años, el 52,9% fueron mayores de 60 años, el tiempo en HDC 21,6 ± 17 meses, la nefropatía diabética fue la etiología más frecuente. El 73,5% de los pacientes presentaron MME. La artrosis y la lumbalgia fueron las más frecuentes. La artrosis se relacionó con edad >60 años (66,7% vs. 12,5%; p=0,000 y la presencia de diabetes mellitus (DM (68,6% vs. 32,7%; p=0,010. En 64,9% de pacientes se encontró densidad mineral ósea (DMO disminuida, 11 (29,7% tuvieron osteoporosis y 12 (32,4% osteopenia. Conclusión: Las MME tienen frecuencia elevada en pacientes en HDC, siendo la artrosis, la lumbalgia y la DMO disminuida las manifestaciones más frecuentes.

Estudio de una tecnología de extracción y fraccionamiento de aceite de ñandú de alta calidad para su utilización en la industria cosmética y farmacéutica

Directory of Open Access Journals (Sweden)

Rosa Márquez

2011-04-01

Full Text Available El propósito de este estudio fue aplicar la metodología de extracción con calor en condiciones de vacío a  grasa cavitaria y exterior para la obtención de aceite de ñandú (Rhea americana con condiciones, una vez refinado,  de humedad, acidez, índice de peróxidos, color y perfil de ácidos grasos compatibles con las exigencias del comercio internacional para los aceites de ratites.En cuanto al método de refinación seleccionado fue el método de refinación alcalina. Las condiciones elegidas para la neutralización fueron: NaOH 10% y 0.1% de exceso de la misma. Luego el aceite se lava hasta un contenido de jabones inferior a 50 ppm debido a que los mismos afectan el valor de acidez. De esta forma se lograron valores de acidez muy por debajo del límite requerido por la AEA (American Emu Association para el aceite de emú extra refinado. Se utilizó tierra de blanqueo Supreme 126 (marca Tonsil de Quimica Sumex S.A., en una concentración del 0.5 %.Se determinó que no existen diferencias sustanciales entre la grasa cavitaria y exterior y en el proceso de winterización se obtienen  dos  fracciones con  perfil de ácidos grasos diferentes siendo la oleína más rica en ácidos grasos insaturados. El contenido de oleico prácticamente no varía entre las fracciones, mientras que el porcentaje de linoleíco (ácido graso esencial es superior para la oleína.En el presente  trabajo se obtuvo un aceite cuyas características y parámetros de calidad son aceptables para los requerimientos internacionales que deben poseer los aceites para aplicación en cosmética, haciéndolo factible de comercializar. Por lo que los métodos de extracción y refinación adaptados para esta aplicación son adecuados y simples de aplicar.

Anti-HBc testing for blood donations in areas with intermediate hepatitis B endemicity Detección de anticuerpos anti-HBc en sangre donada en lugares donde la hepatitis B tiene endemicidad intermedia

Directory of Open Access Journals (Sweden)

Celina Maria Turchi Martelli

1999-07-01

Full Text Available En otros estudios se ha evaluado el menor riesgo de infecciones transmitidas por transfusión sanguínea que acarrea el tamizaje de anticuerpos contra los antígenos nucleares de la hepatitis B (anti-HBc, sumado a la prueba detectora de antígenos de superficie (HbsAg. No obstante, la mayoría de estos estudios se basaron en datos procedentes de zonas con baja endemicidad por hepatitis B o de bancos de sangre de países desarrollados, donde la mayoría de la sangre proviene de personas que han donado previamente. A fin de examinar los pros y los contras del tamizaje de anticuerpos anti-HBc en el Brasil, los autores describen los antecedentes y la interpretación de las pruebas de tamizaje para prevenir la hepatitis postransfusión. También presentan los datos sobre el tamizaje de la hepatitis B en todas las regiones del Brasil. No hay pruebas suficientes para suspender el tamizaje de los anticuerpos anti-HBc en el país. Debe darse alta prioridad a comparar el costo-efectividad de un marcador basado en la biología molecular con el de la actual prueba detectora de anticuerpos anti-HBc aplicada comúnmente, a fin de mejorar las medidas de tamizaje que garantizan la inocuidad de la sangre.

Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con enfermedad de Chagas y leishmaniasis Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas’ disease and leishmaniasis

Directory of Open Access Journals (Sweden)

Gloria Griemberg

2006-02-01

Full Text Available Los anticuerpos anti-ADN nativo pueden detectarse por inmunofluorescencia indirecta con Crithidia luciliae, observándose tinción fluorescente anular del kinetoplasto que contiene ADN de doble cadena. En algunos casos pueden observarse imágenes fluorescentes en flagelo, membrana y corpúsculo basal, consideradas atípicas. Como C. luciliae pertenece a la familia Trypanosomatidae, que incluye patógenos para el hombre como Trypanosoma cruzi y Leishmaniaspp., se consideró que las imágenes atípicas pudieran deberse a reacciones cruzadas. Se realizaron estudios serológicos para Chagas a 105 muestras provenientes de zona endémica (Corrientes y no endémica (Buenos Aires para T. cruzi que presentaban imágenes atípicas con C. luciliae. La serología para Chagas resultó positiva en el 64.7% de las muestras de Buenos Aires y en el 78.3% de las de Corrientes que presentaban frente a C. luciliae imagen conjunta de membrana y flagelo. No presentaron la imagen conjunta ninguna de las muestras de dadores de sangre normales, ni de pacientes con enfermedades del tejido conectivo, excepto dos con lupus que también eran chagásicos. Todas las muestras de pacientes chagásicos analizadas frente a C. luciliae presentaron la imagen conjunta. Se estudiaron también 46 muestras de pacientes con leishmaniasis, 28 de ellos coinfectados con T. cruzi. La imagen conjunta se observó en el 88.0% de las muestras de leishmaniásicos y en el 78.5% de las de coinfectados. Los resultados sugieren que C. luciliae podría ser un sustrato alternativo, económico y de bajo riesgo para el diagnóstico serológico de enfermedad de Chagas, aunque no discrimina la infección por Leishmania. El hallazgo de la imagen conjunta en la detección de anti-ADN nativo señala la conveniencia de realizar en esos pacientes, estudios clínicos y de laboratorio para enfermedad de Chagas y leishmaniasis.Anti-native DNA antibodies can be detected by indirect immunofluorescence assay with

La utopía banal: la urbanización, la globalización y lo anti-bucólico en Últimas noticias del paraíso

Directory of Open Access Journals (Sweden)

Mac John Wilson

2012-01-01

Full Text Available One of the consequences of globalization in Spain has been the import of the so-called Anglo suburban culture coming from the western world. The development of this culture comes from pastoral roots, in that humans seek to live peacefully with each other as well as with non-humans. Nevertheless, as it has come into contact with capitalism, this pastoral culture has failed because it is commodified like any other consumer product. The Spanish residential communities that have inherited this cultural history in part are known as urbanizaciones. In Clara Sanchez's novel, Últimas noticias del paraíso, these communities reveal themselves as places built to bring together humanity and nature but that, from the perspective of a coming-of-age adolescent, in actuality are centers of artificiality condemned to banality. What this current work proposes is that Últimas noticias del paraíso presents itself as an anti-pastoral and in this way underlines the problems that the globalization of capitalistic culture brings with it by spreading alienation and ambivalence on the part of humanity when confronted with the natural world, even while maintaining a pastoral mask.

Sistema analítico de evaluación de la actividad biológica del reactivo hemoclasificador HEMO-CIM ANTI-A Analytical system of evaluation of the biological activity of anti-A hemo-cim hemoclassifier reagent

Directory of Open Access Journals (Sweden)

Lilia Suárez Batista

2000-08-01

Full Text Available Con el objetivo de llevar a cabo el escalado industrial de los productos hemoclasificadores a partir de anticuerpos monoclonales murinos, se realizaron diversas actividades para crear las condiciones que permitieran su manufactura con el cumplimiento de las buenas prácticas de producción en el Centro de Inmunología Molecular. Se introdujeron los métodos analíticos para determinar la calidad del producto hemoclasificador Hemo-CIM anti-A en el Departamento de Control de la Calidad, se entrenó al personal responsable de esta actividad en el uso de estos métodos de evaluación de la actividad biológica del AcM anti-A en el sobrenadante, líquido ascítico y producto terminado; se diseñaron y elaboraron los documentos que forman parte del sistema de documentación; entre ellos, los procedimientos normalizados de operación (PNO, los expedientes maestros de lotes, los documentos para la recolección de datos, así como los registros de lote, los certificados de calidad y los registros de las mediciones de la actividad biológica para archivar los resultados en el laboratorioWith the objective of carrying out industrial scaling of hemoclassifier products from murin monoclonal antibodies, a number of activities were performed to create the conditions for their manufacture in compliance with good manufacturing practices of the Molecular Immunology Center. The analytical methods were introduced to determine the quality of anti-A Hemo-CIM hemoclassifier in the Quality Assurance Department. Staff responsible for this work was trained in the use of these evaluation methods of the biological action of anti-A monoclonal antibody in supernatant, ascitic fluid and finished product; the documents that are part of the documentation system were designed and prepared, among them standard operating procedures, master production batch records, documents for data-gathering, batch records, quality certificates and biological activity measurement registers to

Anti-trypanosomal activity of pentacyclic triterpenes isolated from Austroplenckia populnea (Celastraceae Atividade anti-tripanosomicida de triterpenes pentacíclicos isolados de Austroplenckia populnea (Celastraceae

Directory of Open Access Journals (Sweden)

Lucienir Pains DUARTE

2002-04-01

Full Text Available Four pentacyclic triterpenes isolated from Austroplenckia populnea and four compounds of known anti T. cruzi or anti-malarial activity were tested. Of those triterpenes tested 20alpha-hydroxy-tingenone showed high activity, epikatonic acid was less active, while populnilic and populninic acids were inactive against the trypanosome of the subgenus Schizotrypanum tested. Benzonidazole, nifurtimox, ketoconazole and primaquine presented a remarkable dose-dependent inhibitory effect reaching practically to a total growth inhibition of the parasite at the end of incubation time. The trypanosome tested appear to be a suitable model for preliminary screen for anti T. (S. cruzi compounds.Foram testados quatro triterpenos pentacíclicos isolados de Austroplenckia populnea e quatro compostos de conhecida atividade anti-T. cruzi ou anti-malárica. Dos triterpenos testados 20alfa-hidroxi-tingenona mostrou atividade elevada, ácido epicatônico foi menos ativo, enquanto ácido populnílico e populnínico foram inativos contra o tripanossoma do subgênero Schizotrypanum testado. Benzonidazole, nifurtimox, cetoconazole e primaquina apresentaram efeito inibitório dose-dependente atingindo praticamente a inibição total do crescimento do parasita no final do tempo de incubação. O tripanossoma testado mostrou ser um modelo adequado para uma seleção preliminar de compostos anti. T. (S. cruzi.

Indicadores para seleccionar inóculos de hongos micorrícicos arbusculares eficientes en suelos moderadamente ácidos Indicators to select efficient arbuscular mycorrhizal fungi inocula in moderately acidic soils

Directory of Open Access Journals (Sweden)

F Covacevich

2010-07-01

Full Text Available En ocasiones los propágulos de hongos micorrícicos arbusculares (HMA en los suelos pueden estar en bajo número o éstos no ser eficientes para aumentar el crecimiento de las plantas hospedadoras. La estrategia sería recurrir a la inoculación con HMA (nativos o no. Si la inoculación se realiza con HMA no nativos, debe considerarse que los suelos difieren en su receptividad a los HMA a ser introducidos. El objetivo del trabajo fue seleccionar algunos indicadores de presencia, actividad y beneficio de la simbiosis planta-HMA no nativos para ser utilizados como inóculos en suelos moderadamente ácidos. Para ello, se evaluó cómo la inoculación con HMA incidió sobre la colonización micorrícica y algunos parámetros de crecimiento en una planta modelo crecida en dos suelos moderadamente ácidos de diferente origen (Argentina y Francia. En el suelo de la Argentina la inoculación con Glomus claroideum y Acaulospora longula ocasionaron los mayores grados de micorrización total y con actividad fosfatasa alcalina (ALP así como las mayores respuestas micorrícicas (RM. En el suelo de Francia, el mayor crecimiento y cantidad de raíz micorrizada se obtuvieron por la inoculación con A. longula. La inoculación con Scutellospora pellucida presentó adecuada RM en el suelo de la Argentina, pero nula en el suelo de Francia. G. clarum manifestó una alta capacidad micotrófica, aunque una baja eficiencia para ser utilizado en los suelos testeados. Asimismo, la inoculación con A. laevins presentó los más bajos niveles de colonización y las menores respuestas de crecimiento en ambos suelos. El análisis directo de los parámetros, así como el análisis multivariado entre la RM y los parámetros de micorrización y crecimiento mostraron que la combinación entre los parámetros de peso aéreo y de producción de raíces con los de colonización micorrícica (total y con actividad ALP resulta adecuada para identificar de manera rápida las

Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con enfermedad de Chagas y leishmaniasis Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas’ disease and leishmaniasis

OpenAIRE

Gloria Griemberg; Nidia F. Ferrarotti; Graciela Svibel; Maria R. Ravelli; Néstor J. Taranto; Emilio L. Malchiodi; María C. Pizzimenti

2006-01-01

Los anticuerpos anti-ADN nativo pueden detectarse por inmunofluorescencia indirecta con Crithidia luciliae, observándose tinción fluorescente anular del kinetoplasto que contiene ADN de doble cadena. En algunos casos pueden observarse imágenes fluorescentes en flagelo, membrana y corpúsculo basal, consideradas atípicas. Como C. luciliae pertenece a la familia Trypanosomatidae, que incluye patógenos para el hombre como Trypanosoma cruzi y Leishmaniaspp., se consideró que las imágenes atípicas ...

Los niños del Cauca. I. Descripción de un foco de raquitismo dependiente de la vitamina D, Tipo II.

Directory of Open Access Journals (Sweden)

Alejandro Giraldo

1993-09-01

Full Text Available Al norte del departamento del Cauca se detectó un numeroso grupo de pacientes con deformidades en miembros inferiores (genu varo, genu valgo o ambos que correspondían clínica y radiológicamente a un cuadro de raquitismo. Ninguno de los pacientes presentaban alopecia, miopatia, tetania o aminoaciduria. La mayoría deellos proceden de un grupo poblacional semiaislado de ancestro afromestizo, en el corregimiento de La Toma, del municipio de Suárez. Los estudios bioquímicos comparativos entre 64 pacientes y 109 individuos no afectados, parientes en primer grado, mostraron en los pacientes una ligera hipocalcemia, niveles normales altos de fósforo sérico, un sustancial incremento de la fosfatasa alcalina,valores normales de proteínas y albúminas y niveles séricos de paratohormona aumentados. Estudios en orina de 24 horas mostraron hipocalciuria e hipofosfaturia. A un subgrupo de 8 pacientes se les determino niveles séricos de la 25 (OHD, y lpara establecer a qué tipo de raquitismo pertenecían nuestros pacientes. Se observó un marcado incremento de lcr, 25(OH,A3, por lo que esta patología corresponde al raquitismo dependiente de la vitamina D, tipo II. Se trata de un tipo de raquitismo genético transmitido en forma autocómica recesiva, cuyas manifestaciones clínicas y de laboratorio se presentan por un defecto en la acción del receptor para la vitamina D. A 115 pacientes se les inició tratamiento con 1a,25(OH,A3 hig 0,50/día, calcio 1.840 mg/día y fósforo 1.424 mg/día, administrados en dos dosis diarias. En un grupo de 21 pacientes, se analizaron los datos de laboratorio en suero, pre y posttratamiento un ano después y todos los valores tendieron a normalizarse. Estudios moleculares, del mRNA y cDNA del gen del receptor de la vitamina D, obtenidos de cultivo de fibroblastos de dos de los pacientes más severamente afectados, mostraron una secuencia normal de nucleótidos. Se hace una comparación entre los

EXTRACCIÓN Y CUANTIFICACIÓN DE LOS POLÍSACARIDOS DE LA PARED CELULAR DE LAS LEVADURAS

Directory of Open Access Journals (Sweden)

Elba M. Alcázar V

2016-01-01

Full Text Available Los polisacáridos de la pared celular de las levaduras, se dividen en: 1,3 B - gluc anos, 1,6 B - glucanos, manoproteínas y quitina. Estos componentes representan una fuente potencial de aplicaciones en la industria alimentaria y farmacéutica. Por lo tanto, p ara su extracción existen d iferentes protocolos, en donde para lograr la separación de los componentes de la pared se basan en su solubilidad en soluciones alcalinas y/o e n ácidos inorgánicos, que posteriormente son hidrolizados y usualmente cuantificado por técnicas espectrofotométricas con reactivos de fenol - ácido sulfúrico y/o con el reactivo de antrona . Algunas metodologías sólo extraen los componentes celulares en una fracción total, limitando su cuantificación y análisis para el desarrollo de nuevas aplicaciones en las industrias alimentaría y farmacéutica, mientras que otros método s dividen a la pared celular en sus tres componentes. No obstante, para su cuantificación y análisis utilizan metodologías extensas y complicadas . El objetivo del presente traba jo fue proponer un a metodología de extracción de la pared celular de dos cepas de levadura ( Saccharomyces cerevisiae y Kluyveromyces marxianus a través de una extracción alcalina , dividiendo en tres fracciones los componentes de la pared celular para su posterior cuantifica ción a partir de cromatografía de líquidos. Se encontró que las cepas de levadura empleadas presentan diferencias estadísticas significativas en la concentración de 1,3 B - glucanos, y que los rendimientos obtenidos en la extracción de la pared celular de am bas cepas de levadura son similares a los reportados en estudios previos. Por lo tanto, podemos concluir que la extracción de la pared celular empleando esta metodología es confiable.

O uso de anti-retrovirais em gestantes modifica o perfil lipídico?

Directory of Open Access Journals (Sweden)

El Beitune Patrícia

2003-01-01

Full Text Available OBJETIVO: avaliar o efeito das medicações anti-retrovirais sobre o metabolismo lipídico em gestantes portadoras do HIV. MÉTODOS: estudo prospectivo realizado em 57 gestantes que foram divididas em três grupos: grupo AZT, com 20 pacientes portadoras do HIV em uso de AZT; grupo TT, com 25 pacientes portadoras do HIV em uso de esquemas contendo três anti-retrovirais (AZT + 3TC + NFV e grupo controle, com 12 pacientes. Os dados demográficos e antropométricos foram homogêneos entre os grupos estudados. Foram excluídas as pacientes com antecedentes pessoais ou familiares de hiperlipidemia. Amostras sanguíneas foram obtidas para avaliação dos lipídeos (colesterol total, frações LDL e HDL-colesterol e triglicerídeos em quatro oportunidades durante a gravidez (1º = 14-20 semanas; 2º = 21-26 semanas; 3º = 27-32 semanas e 4º = 33-38 semanas. Os dados foram analisados utilizando-se os testes não paramétricos do chi², teste de Friedman e teste de Kruskal-Wallis. RESULTADOS: o uso de anti-retrovirais durante a gestação não induziu diferenças nas taxas do colesterol total e HDL, no entanto, o uso destes agentes esteve associado a elevação de 76,5 mg/dL e 84 mg/dL para 96 mg/dL e 105 mg/dL na concentração da fração LDL-colesterol para os grupos AZT e TT, respectivamente (p<0,01. Observou-se associação positiva significante entre os triglicerídeos e a carga viral nas gestantes do grupo AZT (r = 0,53; p = 0,015. CONCLUSÃO: a utilização dos anti-retrovirais durante a gestação eleva significativamente a concentração da lipoproteína LDL. Persistem as dúvidas se a gestação potencializa a longo prazo os efeitos dos anti-retrovirais sobre o metabolismo lipídico.

Electrodeposición de películas delgadas de CdTe sobre electrodo de oro en soluciones acuosas de EDTA-Amonio

OpenAIRE

Montilla, Milagro; Alarcón, Domingo; Ortiz, Reynaldo

2007-01-01

En este trabajo se muestran los resultados obtenidos del estudio del crecimiento electroquímico y caracterización de películas delgadas del semiconductor CdTe. El crecimiento se realizó mediante la electrodeposición catódica a potencial constante a partir de soluciones acuosas alcalinas de las especies precursoras TeO3(2-) y Cd2+ y EDTA como agente acomplejante para el Cd2+. La composición de las películas se determinó por espectroscopía de emisión atómica con plasma inductivamente acoplado (...

Planejamento Operacional e Cinética do Processo Heterogêneo de Transesterificação de Oleaginosas Em Biodiesel

OpenAIRE

Silva, Suzana Pedroza da

2012-01-01

Processaram-se óleos vegetais de soja e girassol via transesterificação catalítica alcalina produzindo-se em meios metílico e etílico biodieseis e o coproduto glicerina. Indicou-se alternativa de processo à transesterificação com metanol, evidenciando-se o uso da matéria disponível etanol. Convergindo para as condições práticas de elevados rendimentos em ésteres, buscou-se estabelecer garantias de produção de biocombustíveis de qualidade com maiores graus de pureza. Operações d...

Níveis de fibra em detergente neutro em dietas para eqüinos Neutral detergent fiber levels in diet of equines

Directory of Open Access Journals (Sweden)

Auro César Braga

2008-11-01

Full Text Available Objetivou-se nesta pesquisa avaliar os efeitos de dois níveis de fibra em detergente neutro (FDN da dieta (25 e 35%, provenientes de duas proporções de volumoso/concentrado (50:50 e 60:40, sobre a digestibilidade dos nutrientes, o pH fecal e os parâmetros sangüíneos de lactato, glicose, uréia, creatinina e fosfatase alcalina, visando estabelecer os níveis mínimos e seguros de fibra em dietas para eqüinos. Utilizaram-se cinco eqüinos sem raça definida, com 6 a 8 anos de idade e peso médio de 300 kg, alojados em gaiolas próprias para estudos de metabolismo. Os animais foram distribuídos em delineamento de quadrado latino 5 × 5, composto de cinco tratamentos (dois níveis de FDN, 25 e 35%, duas relações volumoso:concentrado, 50:50 e 60:40, e uma dieta controle, com relação 50:50 volumoso:concentrado e cinco períodos. Cada período experimental teve duração de 15 dias (10 de adaptação e 5 de coleta de fezes e sangue e determinação do pH fecal. Os níveis de FDN utilizados neste experimento (25 e 35% reduziram a digestibilidade dos componentes fibrosos (fração parede celular da dieta, entretanto, não influenciaram os níveis sangüíneos de lactato, glicose, uréia, creatinina e fosfatase alcalina e o pH fecal. A dieta com 25% de FDN promoveu alteração no teor plasmático de fibrinogênio, portanto, pode aumentar a predisposição dos animais a quadros de laminite e cólica.The objective of this research was to evaluate the effects of two levels of neutral detergent fiber (NDF of the diet (25 and 35% from two forage to concentrate (50:50 and 60:40 ratio on nutrient digestibility, fecal pH and blood parameters as lactate, glucose, urea, creatinine, fibrinogen and alkaline phosphatase, to establish minimum and safe levels of fiber in equines diets. Five horses without defined race, with 6 to 8 years old and average 300 kg BW, were housing in metabolism cages. The animals were distributed to 5 × 5 Latin square design

Dosimetry and microdosimetry of {sup 188} Re-anti-CD20 and {sup 131} I-anti-CD20 for the treatment of No Hodgkin lymphomas; Dosimetria y microdosimetria del {sup 188} Re-anti-CD20 y {sup 131} I-anti-CD20 para el tratamiento de linfomas No Hodgkin

Energy Technology Data Exchange (ETDEWEB)

Torres G, E

2007-07-01

The purpose of this investigation was to prepare {sup 131}I-anti-CD20 and {sup 188}Re-anti-CD20 and to estimate the radiation absorbed dose at macro- and micro- level during a NHL treatment. The work was divided in 4 general objectives: 1) preparation of {sup 131}I-anti-CD20 and {sup 188}Re-anti-CD20, 2) application in patients to obtain biokinetic parameters and estimate the organ absorbed doses 3) estimation of the cellular dosimetry using the MIRD methodology and the MCNP4C2 code and 4) estimation of the cellular microdosimetry using the NOREC code. {sup 188}Re-anti-CD20 was prepared by a direct labelling method using sodium tartrate as a weak ligand. To evaluate the biological recognition a comparative study of the in vitro binding of {sup 188}Re-anti-CD20, {sup 125}I-anti-CD20 (positive control) and {sup 188}Re-anti-CEA (negative control) to normal B Iymphocytes was performed. Biodistribution studies in normal mice were accomplished to assess the in vivo Re-anti-CD20 complex stability. The binding of ' Re-anti-CD20 to cells was in the same range as '251-anti-CD20 (>80%) considered as the positive control. {sup 188}Re-anti-CD20 and '3'1-anti-CD20 prepared were administered in patients diagnosed with B cell NHL at the Centro Medico Siglo XXI (IMSS). The protocol was approved by the hospital's Medical Ethics Committee. AJI patients signed a consent form after receiving detailed information on the aims of the study. N data were the input for the OLINDA/EXM software to calculate the radiation absorbed dose to organs and whole body. Dosimetric studies indicate that after administration of 6.4 GBq and 4.87 to 8.75 GBq of '3'1-anti-CD20 and {sup 188}Re-anti-CD20 respectively, the absorbed dose to total body would be 0.75 Gy which corresponds to the recommended dose for NHL therapies. The calculated organ absorbed doses indicate that {sup 188}Re-anti-CD20 may be used in radioimmunotherapy without the risk of toxicity to red marrow or

Dosimetry and microdosimetry of {sup 188} Re-anti-CD20 and {sup 131} I-anti-CD20 for the treatment of No Hodgkin lymphomas; Dosimetria y microdosimetria del {sup 188} Re-anti-CD20 y {sup 131} I-anti-CD20 para el tratamiento de linfomas No Hodgkin

Energy Technology Data Exchange (ETDEWEB)

Torres G, E

2007-07-01

The purpose of this investigation was to prepare {sup 131}I-anti-CD20 and {sup 188}Re-anti-CD20 and to estimate the radiation absorbed dose at macro- and micro- level during a NHL treatment. The work was divided in 4 general objectives: 1) preparation of {sup 131}I-anti-CD20 and {sup 188}Re-anti-CD20, 2) application in patients to obtain biokinetic parameters and estimate the organ absorbed doses 3) estimation of the cellular dosimetry using the MIRD methodology and the MCNP4C2 code and 4) estimation of the cellular microdosimetry using the NOREC code. {sup 188}Re-anti-CD20 was prepared by a direct labelling method using sodium tartrate as a weak ligand. To evaluate the biological recognition a comparative study of the in vitro binding of {sup 188}Re-anti-CD20, {sup 125}I-anti-CD20 (positive control) and {sup 188}Re-anti-CEA (negative control) to normal B Iymphocytes was performed. Biodistribution studies in normal mice were accomplished to assess the in vivo Re-anti-CD20 complex stability. The binding of ' Re-anti-CD20 to cells was in the same range as '251-anti-CD20 (>80%) considered as the positive control. {sup 188}Re-anti-CD20 and '3'1-anti-CD20 prepared were administered in patients diagnosed with B cell NHL at the Centro Medico Siglo XXI (IMSS). The protocol was approved by the hospital's Medical Ethics Committee. AJI patients signed a consent form after receiving detailed information on the aims of the study. N data were the input for the OLINDA/EXM software to calculate the radiation absorbed dose to organs and whole body. Dosimetric studies indicate that after administration of 6.4 GBq and 4.87 to 8.75 GBq of '3'1-anti-CD20 and {sup 188}Re-anti-CD20 respectively, the absorbed dose to total body would be 0.75 Gy which corresponds to the recommended dose for NHL therapies. The calculated organ absorbed doses indicate that {sup 188}Re-anti-CD20 may be used in radioimmunotherapy without the risk of toxicity to red marrow or healthy organs. The absorbed dose

Do Evidence-Based Approaches Alienate Canadian Anti-Trafficking Funders?

Directory of Open Access Journals (Sweden)

Alison Clancey

2014-09-01

Full Text Available As a sex worker support organisation, SWAN (Supporting Women’s Alternatives Network Vancouver’s relationship to anti-trafficking funding remains ambivalent, particularly given the history of anti-trafficking measures that have jeopardised the rights of sex workers. In this article, we share how we, as a small grassroots group, attempt to work through these ambivalences in dialogue with donors. Although SWAN Vancouver works with women who are often perceived to be trafficked (i.e. Asian women in sex work, it is rare for members of SWAN Vancouver to come across any case in the sex-work sector that has the hallmarks of trafficking, such as coerced work. Instead, our anti-trafficking work has mainly involved identifying the harms and human rights violations caused by repressive or misguided anti-trafficking measures. We reflect on our dialogue with two Canadian funders (a federal government agency and a national public foundation that have considerable resources and immense power to influence what anti-trafficking practices are implemented in Canada. We analyse how these two funders and their adoption of an anti-prostitution analysis of trafficking will likely result in punitive consequences for immigrant sex workers, and therefore increase the need to assist women who have been anti-trafficked rather than trafficked. ¿Están los financiadores canadienses contra la trata alejados del enfoque basado en la evidencia? Resumen Como una organización que apoya a los trabajadores sexuales, la relación entre SWAN (Red Alternativa de Apoyo a las Mujeres y los financiadores contra la trata en Vancouver es contradictoria, sobre todo teniendo en cuenta la historia de las medidas contra la trata que han puesto en peligro los derechos de los trabajadores sexuales. En este artículo discutimos cómo nosotros, como un pequeño pero clave grupo especializado, nos esforzamos en trabajar contra estas contradicciones en un diálogo continuo con los donantes

Análise quantitativa e molecular de hemoglobina fetal em indivíduos da população brasileira

Directory of Open Access Journals (Sweden)

Zamaro Paula J. A.

2003-01-01

Full Text Available A hemoglobina fetal - Hb F, formada por duas cadeias gama e duas cadeias alfa, é característica do período fetal do desenvolvimento, tendo sua síntese diminuída no período pós-natal. Em algumas alterações hereditárias, a Hb F permanece aumentada, como nas delta-beta talassemia, beta talassemia e persistência hereditária de Hb F (PHHF. A síntese da globina gama também pode ser estimulada por fatores externos como leucemias, transplantes de medula óssea, induções químicas, dentre outros. Através da observação de Hb F aumentada em doadores de sangue por procedimentos eletroforéticos objetivou-se avaliar a quantidade de Hb F em amostras de sangue de candidatos à doação, visando estabelecer seus limites de normalidade na população de São José do Rio Preto e região, por meio de desnaturação alcalina e cromatografia líquida de alta pressão (HPLC, comparar as metodologias aplicadas e, nos indivíduos com Hb F aumentada, realizar estudos moleculares para identificar as mutações que alteram a expressão dos genes gama. Foram analisadas 208 amostras de sangue, sendo 119 de candidatos à doação e 89 de indivíduos sem sintomas de anemia ou achados hematológicos e com Hb F aumentada como grupo comparativo. Das 119 amostras de candidatos à doação, 110 foram utilizadas para traçar o perfil de normalidade de Hb F, comparando-se as metodologias de desnaturação alcalina e HPLC, onde se obteve a média de 1,48% e de 0,6%, respectivamente. A análise estatística por regressão linear mostrou diferença significativa na comparação entre as duas metodologias aplicadas, sendo a HPLC mais precisa para a quantificação de Hb F. Foram observados nos testes de rastreamento de hemoglobinas anormais nestas 110 amostras de sangue: 16,4% de alfa talassemia, 0,9% com Hb F aumentada, 0,9% com beta talassemia e 0,9% com hemoglobina variante de cadeia delta. Os outros nove doadores de sangue apresentaram Hb F acima de 10% em

manifestação de síndrome anti-sintetase

Directory of Open Access Journals (Sweden)

Mário Pires

2014-03-01

Apresentamos o caso de um homem com diagnóstico efectuado há 13 anos, no contexto de internamento por uma “pneumonia”, com queixas de dispneia para esforços e cansaço fácil com 3 meses de evolução. Durante o internamento é identificada uma doença pulmonar intersticial e, posteriormente surge um quadro de mialgias com elevação marcada das enzimas musculares. A electromiografia mostrou um padrão sugestivo de miopatia e identificou-se anticorpos anti-Jo-1, estabelecendo-se o diagnóstico de síndrome anti-sintetase. Sob terapêutica imunossupressora desde então, mantém seguimento em consulta externa, encontrando-se clinicamente bem.

Perfil de utilización de los anti-factor de necrosis tumoral en pacientes de Colombia

Directory of Open Access Journals (Sweden)

Jorge Machado

2011-03-01

Conclusiones. Los anti-TNF se emplearon a las dosis recomendadas, principalmente en artritis reumatoide y en combinación con otros antirreumáticos. El costo directo del tratamiento se consideró elevado para el sistema sanitario del país.

Occurrence of anti-Brucella abortus and anti-Leptospira spp. antibodies in buffaloes from Paraíba state, Northeastern Brazil

Directory of Open Access Journals (Sweden)

Arthur Willian de Lima Brasil

2015-07-01

Full Text Available The objective of this study was to determine the frequency of animals that tested positive for brucellosis and leptospirosis and the risk factors for these diseases in the State of Paraíba. A total of 136 buffaloes from 14 herds were examined. For brucellosis, we used the buffered acidified plate antigen (BAPA test as screening method and 2-mercaptoethanol as confirmatory test. For leptospirosis, we conducted a microscopic agglutination test (MAT, with a cut-off point of 1:100. Of the animals examined, two [1.5%; 95% CI = 0.4%–5.2%] were positive for brucellosis, and 38 (27.9%; 95% CI = 21.1%–36.0% were positive for leptospirosis. The brucellosis-positive animals were from two (14.3% herds, while nine (64.3% herds had leptospirosis-seropositive animals. The more frequent Leptospira spp. serotypes were Bratislava, Pomona, and Canicola. We concluded that leptospirosis was widespread in buffaloes in the state of Paraíba and suggested that breeding alongside horses and pigs might be na important factor in the spread of leptospirosis-positive animals. The presence of brucellosispositive animals indicated the possibility of negative-impacting measures on disease control in bovines, and it is therefore recommended that greater attention be given to these animals for brucellosis control.

Anti-HBs levels among children and adolescents with complete immunization schedule against hepatitis B virus. A cross-sectional study in Blumenau, State of Santa Catarina, Brazil, 2007-2008 Níveis de anti-HBs entre crianças e adolescentes com o esquema completo de imunização contra o vírus da hepatite B. Um estudo transversal em Blumenau, Estado de Santa Catarina, 2007-2008

Directory of Open Access Journals (Sweden)

Andréa do Livramento

2011-08-01

Full Text Available INTRODUCTION: Vaccination is the main tool for preventing hepatitis B virus (HBV infection; however, following the completion of the vaccination series, the concentrations of anti-HBs can decline over the years and reach levels less than 10mIU/mL. The persistence of protection in these individuals is still unknown. The present study aimed to determine the anti-HBs antibody levels among children and adolescents who had received a complete vaccination course for hepatitis B. METHODS: Antibodies against HBV surface antigen (anti-HBs were tested in 371 individuals aged 10 to 15 years-old. RESULTS: Volunteers who showed undetectable quantities of anti-HBs accounted for 10.2% of the population studied and 39.9% presented antibody titers of less than 10mIU/mL. Anti-HBs > 10mIU/mL were verified in 49.9%. CONCLUSIONS: These results corroborate other studies indicating levels of anti-HBs below 10mIU/mL in vaccinated individuals. Additional studies are required to assess whether this indicates susceptibility to HBV infection and the need and age for booster doses.INTRODUÇÃO: A vacinação é o principal instrumento para prevenir a infecção pelo vírus da hepatite B. Todavia, após a conclusão da série de vacinação, as concentrações de anti-HBs podem diminuir ao longo dos anos e atingir níveis inferiores a 10mUI/mL. A persistência da proteção nestes indivíduos ainda é desconhecida. O presente estudo objetivou determinar os níveis do anticorpo anti-HBs em crianças e adolescentes que receberam o esquema completo de vacinação para a hepatite B. MÉTODOS: O anticorpo para o antígeno de superfície do vírus da hepatite B (anti-HBs foi testado em 371 indivíduos com idade entre 10-15 anos. RESULTADOS: Os voluntários que apresentaram quantidades indetectáveis de anti-HBs corresponderam a 10,2% da população estudada, e 39,9% apresentaram títulos do anticorpo inferiores a 10mUI/mL. Anti-HBs > 10mUI/mL foi verificado em 49,9%. CONCLUS�

[Analytic study of dot blotting for the detection of anti-Jo-1, anti-M2, anti-ribosomes and anti-LKM].

Science.gov (United States)

Huguet, S; Sghiri, R; Ballot, E; Johanet, C

2004-01-01

The Cyto-Dot 4 HM043 kit commercialised by BMD, has replaced the Cyto-Dot HM010 kit that allowed three auto-antibodies detection (anti-Jo-1, anti-M2 and anti-ribosomal protein). Detection of anti-LKM1 auto-antibody was added. These four auto-antibodies have in common only the intracytoplasmic localisation of their respective antigen. The aim of our study was to evaluate this new kit using 104 sera and to compare our results with reference techniques (indirect immunofluorescence IF for anti-M2, anti-ribosomal protein and anti-LKM1, double immunodiffusion ID for anti-Jo-1 and anti-LKM1, western blotting WB for anti-M2) and with Cyto-Dot HM010. The one hundred and four sera were divided into five groups: Group I (n = 12) with anti-Jo-1 detected by ID; Group II (n = 28) with 26 anti-M2 positive by IF and WB, 2 anti-M2 positive only by WB; Group III (n = 10) with anti-ribosomal protein detected by IF 5 of which precipitated by ID; Group IV (n = 32) with anti-LKM1 by IF and ID divided into 18 AIH2 and 14 HCV; Group V (n = 22) consisting of 14 healthy individuals and 8 patients with hypergammaglobulinemia. Results of this study are similar to those of Cyto-Dot HM010 for the three auto-antibodies already in use. Cyto-Dot 4 is a very good anti-LKM1 confirmation method as it is ID. Copyright John Libbey Eurotext 2003.

Los niveles de anticuerpos anti factor plaquetario 4-heparina y el índice 4T para trombocitopenia inducida por heparina

Directory of Open Access Journals (Sweden)

Marta E. Martinuzzo

2012-02-01

Full Text Available La trombocitopenia inducida por heparina (HIT es un efecto adverso del tratamiento con heparina, mediada por anticuerpos anti complejo factor plaquetario 4 (PF4-heparina (HPIA. La HIT es frecuentemente moderada pero pueden desarrollarse complicaciones trombóticas. El diagnóstico precoz es importante. La detección de HPIA por ELISA tiene alta sensibilidad pero baja especificidad (títulos bajos sin significación clínica. El índice de las 4T (índice 4T puede detectar pacientes con alto riesgo de HIT. El propósito del estudio fue correlacionar los niveles de HPIA y el índice 4T de un grupo de pacientes derivados a nuestro centro. Evaluamos 84 pacientes, 34 de ellos desarrollaron trombosis. Cada médico completó un cuestionario clínico que fue remitido con la muestra a nuestro centro. Los cuestionarios fueron analizados por un investigador externo y el índice 4T se calculó previamente al ensayo. Los HPIA se determinaron por un ELISA (Asserachrom HPIA que detecta los 3 isotipos, IgG, IgM e IgA, único reactivo disponible en Argentina. Los resultados se expresaron como porcentaje de absorbancia (%ABS. La correlación del índice 4T con los HPIA fue 0.472 (rho spearman, p < 0.001. Los pacientes con índice 4T ≥ 6 presentaban %ABS mayores que los ≤ 5 (67 vs. 39, p < 0.001. Aquéllos con trombosis presentaron títulos mayores que los que no la desarrollaron (%ABS 59 vs. 39, p = 0.017. En conclusión: Los títulos altos de HPIA medidos por ELISA, que detecta los 3 isotipos, correlacionaron claramente con el índice 4T ≥ 6 y fueron más frecuentes en los pacientes con trombosis, coincidiendo con lo ya descripto para ensayos de ELISA específicos para isotipo IgG.

Identificação de fatores de risco pré-operatórios para disfagia pós-operatória persistente após cirurgia laparoscópica para doença do refluxo gastroesofágico

OpenAIRE

Ribeiro,Maxwel Capsy Boga; Tercioti-Júnior,Valdir; Souza-Neto,João Coelho de; Lopes,Luiz Roberto; Morais,Drausio Jefferson; Andreollo,Nelson Adami

2013-01-01

RACIONAL: Disfagia no pós-operatório é comum após a operação anti-refluxo. No entanto, uma parte dos pacientes relatam disfagia persistente, e técnica cirúrgica inadequada é uma causa bem documentada deste resultado. OBJETIVO: Este estudo retrospectivo avaliou os fatores de risco no pré-operatório para a disfagia persistente após operação anti-refluxo por via laparoscópica. MÉTODOS: Pacientes submetidos à operação anti-refluxo por via laparoscópica pela técnica de Nissen modificada foram aval...

Enfermedad de Von Gierke: Reporte de tres casos

Directory of Open Access Journals (Sweden)

E. Espinosa

2001-07-01

Full Text Available La enfermedad de Von Gierke es un defecto en la glucosa 6 fosfatasa, degrada el glucógeno en el hígado, riñón e intestino. Asociada con hipoglicemia, acidosis láctica e hiperuricemia.

Anti-G with concomitant anti-C and anti-D: A case report in a pregnant woman.

Science.gov (United States)

Yousuf, Rabeya; Mustafa, Ahmad Nasirudin; Ho, Siew-Ling; Tang, Yee-Loong; Leong, Chooi-Fun

2017-01-01

The G antigen of Rh blood group system is present in almost all D-positive or C-positive red cells but absent from red cells lacking D and C antigens. The differentiation of anti-D and anti-C from anti-G is not necessary for routine transfusion; however, during pregnancy, it is important because anti-G can masquerade as anti-D and anti-C with initial antibody testing. The false presence of anti-D will exclude the patient from receiving anti-D immunoglobulin (RhIG) when the patient actually is a candidate for RhIG prophylaxis. Moreover, patients with positive anti-D or anti-G are at risk of developing hemolytic disease of the fetus and newborn and need close monitoring. Thus, proper identification allows the clinicians to manage patients properly. This case report highlights a rare case of anti-G together with anti-D and anti-C in a pregnant woman. This report disseminates knowledge on identification of anti-G and its importance in pregnant women.

Evaluation of nickel-rich alloys for the electrolytic generation of hydrogen in an alkaline medium; Evaluacion de aleaciones ricas en niquel para la generacion electrolitica de hidrogeno en medio alcalino

Energy Technology Data Exchange (ETDEWEB)

Ortiz Verdin, A.A.; Ortega Borges, R.; Trejo Cordova, G.; Meas Vong, Y. [Centro de Investigacion y Desarrollo Tecnologico en Electroquimica, S.C., Pedro Escobedo, Queretaro (Mexico)]. E-mail: aortiz@cideteq.mx

2009-09-15

The simultaneous electrodeposition of Ni with metals such as Zn to form alloys enables obtaining surfaces with high electrocatalytic activity or particular characteristics that resist wear and abrasion. This work presents the results of the study of the electrochemical characterization of different types of nickel electrodeposition and compositions, used as cathodes, and measurements of the electrocatalytic activity in the release of hydrogen in an alkaline medium. Curves I through IV were generated, which were potentiostatically obtained in a nitrogen atmosphere in a NaOH alkaline solution. The morphology of the deposits was evaluated with sweep electron micrscopy(SEM). The results enabled evaluating its potential application as electrode materials for the generation of electrolytic hydrogen in alkaline medium, given the good electrocatalytic activity of nickel-rich materials. [Spanish] El electrodeposito simultaneo del Ni, con metales como el Zn para formar aleaciones, permite obtener superficies con elevada actividad electrocatalitica o con caracteristicas particulares de resistencia al desgaste y abrasion. En este trabajo se presentan los resultados del estudio de la caracterizacion electroquimica de los electrodepositos a base de niquel de diferente naturaleza y composicion utilizandolos como catodos midiendo la actividad electrocatalitica con respecto a la reaccion de desprendimiento de hidrogeno (RDH) en medio alcalino. Se realizaron curvas I vs V obtenidas potenciostaticamente bajo atmosfera de nitrogeno en una solucion alcalina de NaOH. La morfologia de los depositos se evaluo mediante SEM (microscopio electronico de barrido). Los resultados permiten evaluar su potencial aplicacion como materiales de electrodo para la generacion electrolitica de hidrogeno en medio alcalino, dada la buena actividad electrocatalitica de los materiales ricos en niquel.

Síntese e fotosensibilização de nanotubos de titanatos

OpenAIRE

Bem, Vanessa Rodrigues

2011-01-01

Nesta tese é descrita a preparação de nanotubos de titanatos (TNT) via síntese hidrotérmica alcalina, usando uma nova metodologia que evita a utilização de TiO2 cristalino como precursor. Foi estudada a influência da substituição sódio/protão na estrutura, morfologia e propriedades ópticas dos materiais preparados. Os resultados mostraram que a substituição Na+ → H+ resulta numa redução na distância intercamadas dos TNTs, tendo sido medidos valores entre 1.13±0.03 nm e 0.70±0.02 nm para...

Anti-Cyclic Citrullinated Peptide (Anti-CCP and Anti-Mutated Citrullinated Vimentin (Anti-MCV Relation with Extra-Articular Manifestations in Rheumatoid Arthritis

Directory of Open Access Journals (Sweden)

Laura Gonzalez-Lopez

2014-01-01

Full Text Available We evaluated the association between anti-cyclic citrullinated peptide antibodies (anti-CCP and anti-mutated citrullinated vimentin antibodies (anti-MCV with the presence of extra-articular (ExRA manifestations in 225 patients with rheumatoid arthritis (RA. Ninety-five patients had ExRA and 130 had no ExRA. There was no association of anti-CCP and anti-MCV levels with the presence of ExRA as total group (P=0.40 and P=0.91, resp.. Making an analysis of individual manifestations, rheumatoid nodules were associated with positivity for rheumatoid factor (RF; (P=0.01, anti-CCP (P=0.048, and anti-MCV (P=0.02. Instead, RF, anti-CCP, or anti-MCV were not associated with SS, chronic anemia, or peripheral neuropathy. Levels of anti-CCP correlated with the score of the Health Assessment Questionnaire-Disability Index (HAQ-Di (r=0.154, P=0.03, erythrocyte sedimentation rate (ESR; (r=0.155, P=0.03, and RF (P=0.254, P<0.001, whereas anti-MCV titres only correlated with RF (r=0.169, P=0.02. On adjusted analysis, ExRA was associated with longer age (P=0.015, longer disease duration (P=0.007, higher DAS-28 score (P=0.002, and higher HAQ-DI score (P=0.007, but serum levels of anti-CCP and anti-MCV were not associated. These findings show the need to strengthen the evaluation of the pathogenic mechanisms implied in each specific ExRA manifestation.

Estudio de inmunogenicidad para dos vacunas recombinantes contra hepatitis B

Directory of Open Access Journals (Sweden)

O. Juliao

1991-12-01

Full Text Available Este estudio compara la inmunogenicidad (seroconversión, seroprotección e Hiperrespuesta, producida por dos vacunas recombinantes contra la hepatitis B (Engerix-B de Bélgica y Cubana, en dos esquemas (012 y 016 meses, empleando los métodos de cuantificación para Anti-HBsAg (Abbott y Organón, los cuales fueron también comparados. En el estudio participaron 257 voluntarios,  divididos al azar en 4 grupos (dos vacunas, dos esquemas. Resultados: los dos métodos de Abbon y Organon, no presentan diferencias estadísticas significativas. La vacuna cubana muestra una mayor respuesta inmunogénica para dos dosis de vacuna y para el esquema 012. No hay diferencia entre los esquemas 012 y 016 y en el esquema 016 no se ven diferencias estadísticamente significativas con la vacuna Engerix-B. En esta Última el esquema 016 muestra mejores resultados que el 012.

Anti-G with concomitant anti-C and anti-D: A case report in a pregnant woman

Directory of Open Access Journals (Sweden)

Rabeya Yousuf

2017-01-01

Full Text Available The G antigen of Rh blood group system is present in almost all D-positive or C-positive red cells but absent from red cells lacking D and C antigens. The differentiation of anti-D and anti-C from anti-G is not necessary for routine transfusion; however, during pregnancy, it is important because anti-G can masquerade as anti-D and anti-C with initial antibody testing. The false presence of anti-D will exclude the patient from receiving anti-D immunoglobulin (RhIG when the patient actually is a candidate for RhIG prophylaxis. Moreover, patients with positive anti-D or anti-G are at risk of developing hemolytic disease of the fetus and newborn and need close monitoring. Thus, proper identification allows the clinicians to manage patients properly. This case report highlights a rare case of anti-G together with anti-D and anti-C in a pregnant woman. This report disseminates knowledge on identification of anti-G and its importance in pregnant women.

Model independent calculation of B(anti B0→D(*)+τ- anti ν)/B(anti B0→D(*)+e- anti ν)

International Nuclear Information System (INIS)

Hwang, D.S.

2000-01-01

Using the formulas for the dΓ/dq 2 distribution with non-zero lepton mass and experimentally determined form factors, we calculate the dΓ(D (*)+ l - anti ν)/dq 2 spectra and branching fractions for l=e,μ and τ. We obtain the results B(anti B 0 →D + τ - anti ν)/B(anti B 0 →D + e - anti ν)=0.278 +0.049 -0.035 and B(anti B 0 →D *+ τ - anti ν)/B(anti B 0 →D *+ e - anti ν)=0.256 +0.014 -0.013 . Since we used the experimentally measured form factors, these results are independent of theoretical models of the form factors. (orig.)

Enzyme activities of phytoplankton in the South Shetland Islands (Antarctica in relation to nutrients and primary production Actividad enzimática en ensambles fitoplanctónicos en las Islas Shetland del Sur (Antártica en relación a los nutrientes y producción primaria

Directory of Open Access Journals (Sweden)

JOSÉ L IRIARTE

2006-12-01

relaciones entre la nitrato reductasa (NR y la fosfatasa alcalina (FA con la producción primaria y nutrientes inorgánicos en las Islas Shetland del Sur, Antártica. Las variables de actividad enzimática del fitoplancton (0,7-210 µm, producción primaria, biomasa autotrófica y nutrientes inorgánicos fueron analizadas en nueve estaciones en los primeros 100 m de profundidad durante un crucero oceanográfico en el área noroeste de las Islas Shetland del Sur (Antártica, durante la primavera austral (diciembre 2000. Las actividades enzimáticas de NR fluctuaron entre 0 y 42,8 nmol L-1 h-1 (promedio = 10,08 nmol L-1 h-1, DE = 10,42 nmol L-1 h-1 y de FA entre 0,81 y 5,67 nmol L-1 h-1 (promedio = 2,68 nmol L-1 h-1, DE = 0,95 nmol L-1 h-1. Aquellas estaciones con estimaciones de productividad primaria (PP y clorofila a mayores que mg C m-3 h-1 y 0,75 mg clorofila a L-1, respectivamente, presentaron mayores actividades enzimáticas de nitrato reductasa y fosfatasa alcalina que aquellas estaciones caracterizadas por estimaciones de producción primaria y clorofila a menores que 2 mg C m-3 h-1 y 0,17 mg clorofila a L-1, respectivamente. La actividad específica de FA fue negativamente correlacionada con ortofosfato a concentraciones menores que 2,0 µM, lo que sugiere que el fitoplancton se encontraba en condiciones ambientales de deficiencia de fosfato. Nuestros resultados indican que la actividad específica de NR estuvo positivamente asociada a la biomasa autotrófica y producción primaria en la zona fótica, lo que evidencia el uso de nitrato como nutriente nitrogenado por el fitoplancton en aguas superficiales. Además, altas actividades de NR fueron positivamente correlacionadas con NO3-, lo que sugiere la ocurrencia del proceso de respiración de nitrato en aguas superficiales bien oxigenadas de la Antártica

What is it really? Anti-G or Anti-D plus Anti-C: Clinical Significance in Antenatal Mothers.

Science.gov (United States)

Das, Soumya; Shastry, Shamee; Murugesan, M; B, Poornima Baliga; Shastry, Shamee

2017-06-01

G antigen of Rh blood group system is present either along with D and/or C positive red cells. Hence, [serologically anti-G presents with the similar picture as that of multiple antibodies (anti-D + anti-C). Differentiating them is important as anti-D + anti-C causes severe hemolytic disease of the fetus and newborn than anti-G. In pregnancies with anti-G alone, alloimmunization due to D antigen could be prevented by prophylactic administration of RhIg. Differentiating between anti-D + C from anti-G in alloimmunized pregnant mothers becomes essential. Sera from antenatal mothers, whose antibody identification by 11-cell panel gave a pattern for anti-D and anti-C were selected. Extended phenotyping for Rh system was performed for these antenatal cases. Differential adsorption and elution testing using R 2 R 2 cells initially and r'r cells subsequently were performed to distinguish anit-G from anti-D + anti-C. Antibody titers of these antibodies were determined and their clinical outcome in the newborn was followed. A pattern suggestive of anti D and anti C on antibody identification were observed in six antenatal cases. On further workup 50 % of them confirmed to have anti G. Antibody titers of anti-G and anti-C were lower than that of Anti-D. All newborns were sensitized in vivo and the antibody specificity in them were confirmed with elution studies. The mothers who had only anti-G were subsequently administered with an appropriate dose of RhIg.Differential adsorption and elution studies help in identifying anti-G and distinguishing it from anti-D plus anti-C, thus helping in better patient management.

Prevention of mother to child HIV transmission Prevención de la transmisión perinatal de HIV

Directory of Open Access Journals (Sweden)

Adriana S. Duran

2006-02-01

infección por HIV por vía sexual. El diagnóstico de infección por HIV fue previo al de embarazo en el 38.5% de los casos. Un total de 241 pacientes recibieron algún tipo de terapia antirretroviral durante el embarazo y 123, recibieron terapia antirretroviral combinada. El índice de transmisión global fue de 9.6%, y el uso de terapia antirretroviral fue el factor más significativo asociado al índice de transmisión. El odds ratio (OR para la transmisión vertical del HIV fue de 0.04 para cualquier tipo de tratamiento antirretroviral versus la ausencia de tratamiento. No se detectaron casos de transmisión entre las mujeres que recibieron terapia combinada. Los efectos secundarios más frecuentes asociados a la terapia fueron: anemia, hipercolesterolemia, aumento en los niveles de fosfatasa alcalina e hipertrigliceridemia. En conclusión, la terapia antirretroviral, especialmente la terapia combinada, se asoció con reducción en el riesgo de transmisión vertical del VIH, independientemente del tipo de parto. No se detectó mayor toxicidad o incidencia de malformaciones congénitas, en el corto plazo.

The tardi-Pan-African granitoids of South-Westerner Anti-Atlas (Morocco: Evolution from magnesian to ferroan type. Example of the Ifni inlier

Directory of Open Access Journals (Sweden)

El Aouli, E. H.

2007-06-01

SGO y que está constituido por granitos y sienogranitos subvolcánicos de tipo férrico y afinidad calcoalcalina a alcalina. Estos magmatismos provenientes de la fusión de materiales mixtos marcan el final del último episodio panafricano y se habrían emplazado en un contexto transicional entre los estadios orogénico panafricano y anorogénico que anuncia el ciclo Paleozoico. Este emplazamiento sería contemporáneo a posterior a la exhumación de los domos metamórficos extensionales del Anti-Atlas occidental.

Rabbit antibodies for hormone receptors and HER2 evaluation in breast cancer Anticorpos de coelho para avaliação de receptores hormonais e HER2 em câncer de mama

Directory of Open Access Journals (Sweden)

Rafael Malagoli Rocha

2009-01-01

Full Text Available BACKGROUND: Novel rabbit monoclonal antibodies (RabMab for estrogen (ER, progesterone (PR receptors and HER2 evaluation by immunohistochemistry have recently been commercially released. We compared the RabMab anti-ER, anti-PR and anti-HER2 to mouse monoclonal antibodies (Mab using tissue microarrays (TMA of breast carcinomas. METHODS: Two TMA containing breast carcinomas were built. Sections were immunostained using anti-ER and anti-PR, Mab and RabMab. The sections stained for ER and PR were evaluated considering positive those tumors in which more than 1% of the tumor cell nuclei stained moderate or strong. For HER2, the immunostained sections were evaluated using the ASCO/CAP guidelines for HER2. Chromogenic in situ hybridization (CISH was used as the gold standard for HER2 evaluation. CISH was evaluated using the Zymed HER2 CISH interpretation guidelines. RESULTS: RabMab against ER have similar staining patterns compared to the 6F11 (Mab, but stronger than 1D5 (Mab from three different suppliers. The RabMab against PR provide stronger and sharper immunohistochemical signals compared to Mab. The detection of HER2 protein overexpression was more prevalent with the polyclonal antibodies and RabMab than with the Mab. These were more specific than the RabMab, which were more sensitive when compared to CISH. CONCLUSION: The novel RabMab against ER and PR showed higher intensity of staining than the Mab. The RabMab against HER2 is more sensitive than Mab, however, Mab presented more specificity than RabMab when compared to CISH for HER2 evaluation of breast carcinomas.OBJETIVOS: Novos anticorpos monoclonais de coelho (RabMab para a avaliação imuno-histoquímica de receptores de estrógeno (RE, progesterona (RP e HER2 foram lançados comercialmente. Comparamos os RabMab anti-RE, anti-RP e anti-HER2 com os anticorpos monoclonais de camundongo (Mab utilizando tissue microarrays (TMA de carcinomas de mama. MÉTODOS: Foram construídos dois TMAs de

Anti-1,2-Diols via Ni-Catalyzed Reductive Coupling of Alkynes and α-Oxyaldehydes

Science.gov (United States)

Luanphaisarnnont, Torsak; Ndubaku, Chudi O.; Jamison, Timothy F.

2008-01-01

Ni-catalyzed reductive coupling of aryl alkynes (1) and enantiomerically enriched α-oxyaldehydes (2) afford differentiated anti-1,2-diols (3) with high diastereoselectivity and regioselectivity, despite the fact that the methoxymethyl (MOM) and para-methoxybenzyl (PMB) protective groups typically favor syn-1,2-diol formation in carbonyl addition reactions of this family of aldehydes. PMID:15987174

Efeito de anti-inflamatórios não esteroidais na apoptose de células epidermais lamelares de equinos com laminite

Directory of Open Access Journals (Sweden)

C.A.P. Gonçalves

2013-10-01

Full Text Available O objetivo deste trabalho foi avaliar a influência da administração dos anti-inflamatórios não esteroidais (AINEs cetoprofeno, fenilbutazona e flunixin meglumine sobre o índice apoptótico de células epiteliais do tecido lamelar de cavalos com laminite induzida por administração de amido. Foram empregados 20 equinos hígidos, divididos em quatro grupos experimentais (n=5: solução salina, cetoprofeno, fenilbutazona e flunixin meglumine. O tecido lamelar foi coletado por biópsia, fixado e corado pela técnica de TUNEL. À marcação positiva por essa técnica adicionou-se a avaliação morfológica celular para identificação da apoptose. Não houve diferença significativa no índice apoptótico entre os grupos tratados com anti-inflamatórios e o controle (P>0,05. Os anti-inflamatórios não interferiram sobre o índice apoptótico possivelmente porque foram administrados após a fase prodrômica da laminite e/ou porque não são eficazes em alterar a dinâmica da apoptose. Concluiu-se que a administração de anti-inflamatórios não esteroidais após a fase prodrômica da laminite não contribui para uma intervenção no curso da apoptose no tecido lamelar de cavalos com laminite quando comparados ao grupo controle não tratado. Outros estudos, com diferentes períodos de avaliação, são necessários para esclarecer os efeitos dos anti-inflamatórios não esteroidais na fisiopatologia da laminite em equinos, especialmente no que concerne à participação da apoptose.

Desenvolvimento, caracterização e análise sensorial de formulações alimentares com proteínas do soro de leite ou albumina para crianças

Directory of Open Access Journals (Sweden)

Marina Andrade BATISTA

2015-03-01

Full Text Available O presente trabalho visou verificar a elaboração, a caracterização e a avaliação da aceitação de duas formulações alimentares achocolatadas (uma contendo albumina e a outra, concentrado proteico de soro de leite - WPC para crianças de 7 a 10 anos. O desenvolvimento das formulações baseou-se nas recomendações nutricionais para a faixa etária. Foram avaliados os seguintes parâmetros físico-químicos: composição química, estabilidade, pH, viscosidade e fluidez. A análise sensorial englobou as escalas hedônica de atitude, intenção de consumo e doçura e foi realizada por um grupo de 142 julgadores de um centro universitário, com média de 24,5 ± 7,3 anos de idade e 66,2% do sexo feminino. Todos os dados obtidos foram submetidos à análise estatística, adotando-se p 0,05. Observaram-se 3,88 (albumina e 4,79 (WPC vezes mais proteínas em 100 mL de amostra do que em bebidas comerciais. A primeira formulação mostrou-se mais viscosa (137,33 cP versus 22,33 cP da formulação com WPC e menos fluida (percorreu 40 mL em 69 segundosversus 29 segundos da formulação com WPC. Quanto ao pH, o produto com albumina apresentou característica alcalina (8,60 e o com WPC ácida (6,28. Ambas as formulações permaneceram estáveis e homogêneas após 24 horas de visualização. A análise sensorial mostrou que as formulações foram similares em todos os testes aplicados (p > 0,05, sendo que as características mais bem avaliadas foram o sabor, a textura e a doçura, para ambas. Houve boa aceitação das formulações, com nota mediana de 7 e valores acima de 80% para a soma das respostas positivas nos testes. Conclui-se que as formulações alimentares analisadas apresentaram boas características físico-químicas e mostraram-se equilibradas nutricionalmente, com potencial para comercialização.

Aprovechamiento del subproducto sólido de la digestión anaerobia del bagazo de fique (furcraea macrophylla) para el acondicionamiento de suelos

OpenAIRE

Rivera González, Dayana; Plata Martínez, Lorena; Castro Molano, Liliana; Guzmán Luna, Carolina; Escalante Hernández, Humberto

2012-01-01

La digestión anaerobia del bagazo de fique (Furcraea macrophylla) genera 0,3m³ CH4/kg sólidos volátiles (SV) y 7L de lodo efluente por kg de bagazo tratado. El lodo efluente (LE) puede ser empleado en agricultura. El objetivo de este trabajo de investigación fue producir un biosólido a partir de la estabilización alcalina del LE y evaluar el efecto reparador en un suelo franco-arenoso de Mogotes -Santander. El desarrollo metodológico consistió en caracterizar fisicoquímica y microbiológicamen...

Soroepidemiologia para o virus da hepatite B (VHB em gestantes/parturientes e sua transmissão para recém-nascidos em Goiânia, GO

Directory of Open Access Journals (Sweden)

Divina das Dores P. Cardoso

1996-08-01

Full Text Available Foram coletadas, entre março de 1990 e julho de 1992, 1459 amostras sanguíneas de mulheres gestantes/parturientes na cidade de Goiânia-GO, objetivando detecção da infecção pelo vírus da hepatite B (VHB, através dos marcadores sorológicos AgHBs e anti-HBs. O percentual depositividade encontrado, pelo teste imunoenzimãtico, foi de 7,5%, sendo 0,5%para AgHBs e 7,0%para anti-HBs. A análise efetuada, considerando a faixa etária, mostra que 7 de 8 mulheres AgHBs-positivas pertenciam à faixa etária de até 30 anos, situação semelhante em relação ao anti-HBs(83/101. Das 8 mulheres positivas, 4 tiveram seus recém-nascidos submetidos a tratamento profilático com vacina (Engerix B e imunoglobulina humana anti-hepatite B (HBIG. Além disso, 3 dessas crianças foram analisadas sorologicamente, sendo que uma era AgHBs-positiva ao nascimento. Doença sexualmente transmissível e transfusão sanguínea foram fatores de risco que coirelacionaram significantemente com a infecção. Esses resultados parece-nos reforçar a indicação de triagem à infecção pelo vírus da hepatite B no período pré-natal, assim como a adoção de medidas imunoprofiláticas nas crianças nascidas de mães positivas.In order to detect hepatitis B vírus (HBV, 1459 serum samples from pregnant/parturient women were collected at two public hospitals in Goiânia, GO. These samples were tested by enzyme linked immunosorbent assay forHBsAg and anti-HBs. 109 (7.5% serum samples were positive. Eight (0.5% sera were positive for HBsAg and 101 (7.0% for anti-HBs. Viral positivity for both HBsAg and anti- HBs were observed in women which age ranged from 15 to 30 years. Four newborns from HBsAg positive mothers were submitted to the treatment with HBV vaccine (Engerix B and with hyperimmune gammaglobulin (HBIG - Abbott Laboratories - Brazil. Cord blood from one of the newborns was positive for HBsAg. A positive association was found between hepatitis B and sexually

Anti-mutated citrullinated vimentin (anti-MCV) and anti-65 kDa heat shock protein (anti-hsp65): new biomarkers in ankylosing spondylitis.

Science.gov (United States)

Bodnár, Nóra; Szekanecz, Zoltán; Prohászka, Zoltán; Kemény-Beke, Adám; Némethné-Gyurcsik, Zsuzsanna; Gulyás, Katalin; Lakos, Gabriella; Sipka, Sándor; Szántó, Sándor

2012-01-01

Citrullination as well as anti-citrullinated protein/peptide antibodies (ACPA) have been implicated in the pathogenesis of rheumatoid arthritis (RA). While ACPAs are specific and sensitive markers for RA, there have been hardly any reports regarding ACPAs in ankylosing spondylitis (AS). The possible role of antibodies to Mycobacterial 65 kDa heat shock protein (hsp65) has not been characterized in AS. As new laboratory biomarkers of AS are needed, we investigated the prevalence of anti-mutated citrullinated vimentin (MCV) and anti-hsp65 antibodies in AS. Altogether 43 AS and 44 healthy controls were included in the study. Anti-MCV and anti-hsp65 were determined in sera by commercial and in-house ELISA, respectively. Serum autoantibody levels were correlated with ESR, CRP, HLA-B27 status, smoking habits, pain intensity, BASDAI, BASFI and BASMI indices. Patients with AS had significantly higher serum anti-MCV levels (17.3 U/mL, range: 8.3-31.5 U/mL) in comparison to healthy subjects (8.9 U/mL, range: 5.4-13.3 U/mL) (p20 U/mL). The mean anti-hsp65 concentration in AS sera was 124.8 AU/mL (range: 27.2-1000 AU/mL), while controls exerted significantly lower anti-hsp65 levels (mean: 51.8 AU/mL; range: 22.5-88.5 AU/mL) (p<0.001). Correlation analysis revealed that both anti-MCV positivity (r=0.613; p=0.012) and absolute serum anti-MCV levels (r=0.553; p=0.021) correlated with anti-hsp65 levels. Anti-MCV positivity also correlated with ESR (r=0.437; p=0.03). Anti-MCV and anti-hsp65 may be novel biomarkers in AS. Copyright © 2011. Published by Elsevier SAS.

Investigation of the decays anti B0 → D*+l-anti ν and anti B → D**l-anti ν

International Nuclear Information System (INIS)

Albrecht, H.; Ehrlichmann, H.; Hamacher, T.; Hofmann, R.P.; Kirchhoff, T.; Nau, A.; Nowak, S.; Schroeder, H.; Schulz, H.D.; Walter, M.; Wurth, R.; Appuhn, R.D.; Hast, C.; Kolanoski, H.; Lange, A.; Lindner, A.; Mankel, R.; Schieber, M.; Siegmund, T.; Spaan, B.; Thurn, H.; Toepfer, D.; Walther, A.; Wegener, D.; Britton, D.I.; Charlesworth, C.E.K.; Edwards, K.W.; Hyatt, E.R.F.; Kapitza, H.; Krieger, P.; MacFarlane, D.B.; Patel, P.M.; Prentice, J.D.; Saull, P.R.B.; Tzamariudaki, K.; Van de Water, R.G.; Yoon, T.S.; Ressing, D.; Schmidtler, M.; Schneider, M.; Schubert, K.R.; Strahl, K.; Waldi, R.; Weseler, S.

1993-01-01

Exclusive semileptonic B decays with a D *+ meson in the final state have been studied using the ARGUS detector at the DORIS II storage ring. The branching ratio for the decay anti B 0 →D *+ l - anti ν, where l - is either e - or μ - , has been measured to be (5.2±0.5±0.6)%. A significant rate for the decay anti B→D ** l - anti ν has been observed. From an angular analysis of the cascade anti B 0 →D *+ (→D 0 π + )l - anti ν the forward-backward asymmetry A FB and the D *+ polarization parameter α have been determined to be A FB =0.20±0.08±0.06 and α=1.1±0.4±0.2. (orig.)

Desarrollo de improntas para el diagnóstico del virus Epstein-Barr por inmunofluorescencia indirecta Development of slides for Epstein-Barr virus diagnosis by indirect immunofluorescence

Directory of Open Access Journals (Sweden)

Germán R. Pérez

2005-08-01

Full Text Available El virus de Epstein-Barr (VEB es el principal agente oncogénico linfotrópico dentro de la familia Herpesviridae y se encuentra mundialmente distribuido. La primoinfección se produce en adultos jóvenes y se manifiesta como mononucleosis infecciosa. La detección de anticuerpos anti-viral capside antigen (VCA indica infección previa o presente con VEB. Además, se observan títulos elevados de anticuerpos anti-VCA en las enfermedades neoplásicas asociadas al VEB como los linfomas, en individuos HIV-positivos. El objetivo de este estudio fue el desarrollo y puesta a punto de improntas de células P3HR1 para la detección serológica del VEB por técnicas de inmunofluorescencia indirecta (IFI. Se estimularon cultivos de células P3HR1 en crecimiento exponencial con phorbol-12-mirystoil-13-acetato, y se recolectaron alícuotas a distintos tiempos para realizar improntas. Se realizó una IFI con cada impronta usando como anticuerpo primario un suero VEB-positivo. Se observó un aumento del 11% en la expresión del VCA a las 40 horas post-estimulación, decayendo al 3.5% a las 48 horas. Estos datos fueron corroborados por ensayo de western blot con inmunodetección. La precisión intra- e inter-lote de las improntas fue evaluada para anticuerpos IgM e IgG, con sueros probados previamente por equipos para esta determinación disponibles en el mercado para el VEB y con sueros reactivos para otros miembros de la familia Herpesviridae. No se obtuvieron resultados falsos-negativos ni falsos-positivos para el VEB ni se observó reactividad cruzada con otros herpesvirus. Las improntas desarrolladas constituyen un instrumento para el diagnóstico de la primoinfección del VEB y la detección serológica de anticuerpos IgG anti-VCA de neoplasias asociadas al VEB.Epstein-Barr virus (EBV is the main oncogenic lymphotropic agent of the Herpesviridae family and is globally distributed. EBV acute infection occurs in young adults producing infectious

HIV testing among pregnant women in Brazil: rates and predictors Prueba anti-HIV en mujeres embarazadas en Brasil: tasas y predictivos Testagem anti-HIV em mulheres grávidas no Brasil: taxas e preditores

Directory of Open Access Journals (Sweden)

Valdiléa G Veloso

2008-10-01

intervenciones de prevención del HIV durante el embarazo, con la utilización de una encuesta. Se realizaron análisis bivariadas y multivariadas para verificar los efectos independientes de las co-variables en la oferta y realización de la prueba anti-HIV. Los datos fueron colectados en el período de noviembre de 1999 a abril de 2000. RESULTADOS: La realización de la prueba anti-HIV durante el embarazo fue realizada por el 77,5% de las encuestadas. La oferta de la prueba fue positivamente asociada con: el conocimiento previo sobre la prevención de la transmisión materno-infantil del HIV; mayor número de visitas prenatal; mayor nivel de escolaridad y presencia de color blanco de piel. La tasa de aceptación de la prueba anti-HIV fue de 92,5%. CONCLUSIONES: Los resultados indican que La diseminación de la información sobre prevención de la transmisión materno-infantil del HIV puede contribuir para aumentar la cobertura de la evaluación anti-HIV durante el embarazo. Las mujeres no-blancas con menores niveles de educación deben ser priorizadas. La estrategias para aumentar la participación de poblaciones vulnerables al cuidado prenatal y la sensibilización de trabajadores de la salud son de gran importancia.OBJETIVO: Estimar as taxas de oferta e realização do teste anti-HIV e seus preditores entre mulheres que receberam atendimento pré-natal. MÉTODOS: Foi conduzido estudo transversal, de base populacional, com 2.234 puérperas em 12 cidades do Brasil. Amostras probabilísticas foram selecionadas independentemente por cidade, entre puérperas que compareceram a pelo menos uma visita pré-natal. Foram coletados dados sociodemográficos, informações sobre cuidado pré-natal e acesso a intervenções de prevenção do HIV durante a gravidez corrente, com a utilização de um questionário. Foram realizadas análises bivariadas e multivariadas para verificar os efeitos independentes das covariáveis na oferta e realização do teste anti-HIV. Os dados foram

Detection of Anti-Paracoccidioides brasiliensis antibodies in suspected tuberculosis patients = Detecção de anticorpos anti-Paracoccidioides brasiliensis em pacientes suspeitos de tuberculose

Directory of Open Access Journals (Sweden)

Maria Luiza Dias Fraga Peron

2010-07-01

Full Text Available Paracoccidioidomycosis (PCM is an important systemic mycosis in LatinAmerica that occurs as active disease in 1-2% of Paracoccidioides brasiliensis infected people. Like PCM, tuberculosis (TB affects mainly the lungs and the clinical and radiological aspects do notalways allow differentiation between them. The aim of this study was to carry out serological investigation for detecting anti-P. brasiliensis antibodies, by three serological methods, in patientswith symptoms suggestive of pulmonary TB. From August 2005 to September 2006, 76 patients with pulmonary symptoms suspected for TB were attended at the Regional Specialties Center Laboratory in the city of Paranavaí, Paraná, Brazil and submitted to microbiological TB research, ELISA, immunodiffusion and immunoblotting for PCM. Of all the individuals, 21 (27.63% were reactive to P. brasiliensis by ELISA and 11 (14.47% showed a laboratory diagnosis of pulmonary TB. Of all the individuals serologically reactive to P. brasiliensis, by ELISA, none had positive results by immunodiffusion and one reacted with antigen 43 kDa when Immunobloting was carried out. Our results lead us to reflect a necessity to obtain a more specific serologic test for diagnosis of PCM disease in patients with respiratory symptoms considering the high number of individuals reactive to P. brasiliensis especially in endemic areas.Paracoccidioidomicose (PCM é importante micose sistêmica na América Latina, que ocorre como doença ativa em 1-2% dos indivíduos infectados com Paracoccidioides brasiliensis. Assim como a PCM, a tuberculose (TB afeta principalmente os pulmões, porém os aspectos clínicos e radiológicos nem sempre permitem a diferenciação entreessas doenças. O objetivo deste estudo foi realizar um inquérito sorológico para a detecção de anticorpos anti-P. brasiliensis, utilizando três métodos sorológicos, em pacientes com sintomassugestivos de tuberculose pulmonar. De agosto de 2005 a setembro de

Determinación de anticuerpos anti Neospora caninum en vacas de la provincia de Sugamuxi, Colombia

Directory of Open Access Journals (Sweden)

Martín Orlando Pulido Medellín

2013-01-01

Full Text Available El objetivo fue determinar la presencia de anticuerpos anti Neospora caninum en vacas mediante la técnica de ELISA indirecta en el valle de Sugamuxi, Colombia, identificando las variables que influyen en la susceptibilidad de los bovinos al protozoo. Se tomaron muestras de sangre a 400 hembras bovinas y fueron evaluadas por medio de ELISA indirecta. Se determinó una seroprevalencia de Neospora caninum del 57.5 %; no se halló relación entre las hembras en gestación y la presencia del parásito; para la presencia de celo se encontró que el 27.7 % de vacas infectadas por el parásito presentaron repetición de éste. La presencia del parásito fue del 100 % en hembras con historial de cinco partos. Los resultados de este estudio muestran la presencia de anticuerpos anti Neospora caninum en los animales pertenecientes a fincas lecheras de la provincia de Sugamuxi, y la alta prevalencia muestra el riesgo potencial para este tipo de explotaciones.

Determinación de anticuerpos anti-β2glicoproteína I en pacientes con síndrome antifosfolípido Anti- β2 glycoprotein antibodies in patients with antiphospholipid syndrome

Directory of Open Access Journals (Sweden)

Oscar Uribe Uribe

2004-09-01

Full Text Available EL objetivo de este estudio fue comparar la presencia de anticuerpos anti-β2glicoproteína I (anti- β2GPI con las pruebas convencionales de laboratorio de anticuerpos anticardiolipina (aCL y anticoagulante lúpico, y con las manifestaciones clínicas del síndrome antifosfolípido (SAF. Se incluyeron en el estudio 80 mujeres con SAF; 35 de ellas de la consulta de Reumatología y las otras 45 con historia de aborto recurrente espontáneo (ARE; 5 mujeres de la consulta de Reumatología sin SAF, 27 mujeres con ARE, sin SAF y un grupo control de 20 mujeres sanas en edad reproductiva. Se investigaron la presencia de anticuerpos IgG e IgM anticardiolipina (aCL e IgG anti- β2GPI por la técnica de ELISA, y el anticoagulante lúpico por la determinación del tiempo parcial de tromboplastina activado. Adicionalmente, se registraron las manifestaciones clínicas asociadas al SAF. De las pacientes con SAF, 25.7% del grupo de Reumatología (9/35 y 4.4% de las pacientes con ARE (2/45 fueron positivas para anticuerpos anti-β2GPI, mientras que ninguna de las mujeres sin SAF, ni de las mujeres del grupo control, fue positiva. La asociación entre la presencia de anti- β2GPI y los anticuerpos IgG e IgM aCL mostró una diferencia significativa en los títulos de 3+ (altamente positivos en contraste con los individuos negativos para anti- β2GPI. La positividad del anticoagulante lúpico también se correlacionó con la presencia de anticuerpos anti- β2GPI. No hubo diferencia significativa entre las diversas manifestaciones clínicas del SAF y la presencia de dichos anticuerpos. En conclusión, la determinación de anticuerpos anti- β2GPI tiene una alta especificidad en pacientes con SAF pero no se asoció con ninguna manifestación clínica en particular. The objective of this study was to compare the presence of anti-β2glycoprotein (anti- β2GPI antibodies with the conventional laboratory tests of anticardiolipin antibodies (aCL and lupus anticoagulant

Partial wave analysis of anti pp → anti ΛΛ

International Nuclear Information System (INIS)

Bugg, D.V.

2004-01-01

A partial wave analysis of PS185 data for anti pp → anti ΛΛ is presented. A 3 S 1 cusp is identified in the inverse process anti ΛΛ→ anti p p at threshold, using detailed balance to deduce cross sections from anti pp → anti ΛΛ. Partial wave amplitudes for anti pp 3 P 0 , 3 F 3 , 3 D 3 and 3 G 3 exhibit a behaviour very similar to resonances observed in Crystal Barrel data. With this identification, the anti pp → anti ΛΛ data then provide evidence for a new I=0, J PC =1 - resonance with mass M = 2290 ±20 MeV, Γ= 275 ±35 MeV, coupling to both 3 S 1 and 3 D 1 . (orig.)

Triply heavy tetraquark states with the QQ anti Q anti q configuration

Energy Technology Data Exchange (ETDEWEB)

Chen, Kan; Liu, Xiang [Lanzhou University, School of Physical Science and Technology, Lanzhou (China); Lanzhou University and Institute of Modern Physics of CAS, Research Center for Hadron and CSR Physics, Lanzhou (China); Liu, Yan-Rui; Wu, Jing [Shandong University, School of Physics and Key Laboratory of Particle Physics and Particle Irradiation (MOE), Jinan (China); Zhu, Shi-Lin [Peking University, School of Physics and State Key Laboratory of Nuclear Physics and Technology, Beijing (China); Collaborative Innovation Center of Quantum Matter, Beijing (China); Peking University, Center of High Energy Physics, Beijing (China)

2017-01-15

In the framework of the color-magnetic interaction, we systematically investigate the mass splittings of the QQ anti Q anti q tetraquark states and estimate their rough masses in this work. These systems include the explicitly exotic states cc anti b anti q and bb anti c anti q and the hidden exotic states cc anti c anti q, cb anti b anti q, bc anti c anti q, and bb anti b anti q. If a state around the estimated mass region can be observed, its nature as a genuine tetraquark is favored. The strong decay patterns shown here will be helpful to the experimental search for these exotic states. (orig.)

Frequency of anti-HCV, Hbsag and related risk factors in pregnant women at Nishtar Hospital, Multan

International Nuclear Information System (INIS)

Taseer, I.H.; Ishaq, F.; Hussain, L.; Safdar, S.; Mirbahar, A.M.; Faiz, S.A.

2010-01-01

Background: Viral hepatitis is a global issue. Among the hepatitis viruses hepatitis B and C are important in South Asia including Pakistan. There are various modes of transmission of these viruses. Vertical transmission is also gaining importance. Antepartum screening for HBV and HCV would help the infected women for appropriate antiviral therapy at appropriate time as well as for taking proper care of the newborns. The present study was designed to see the frequency of HBsAg and anti-HCV in pregnant women at Nishtar Hospital, Multan. Methods: This was a cross-sectional study carried out using non-probability purposive sampling technique. The period of the study was from June 2006 to August 2007. Five hundred (500) pregnant women attending outpatient department of Gynaecology and Obstetrics were included. Informed consent was taken. A specially designed proforma was filled in. Anti-HCV and HBsAg were tested by device method. Data were analyzed on SPSS-11. Results: Out of 500 pregnant women 35 (7.00%) were found to be anti-HCV positive and 23 (4.60%) were positive for HBsAg. Mean age was 26.7+-4.8 years. Majority of the patients 263 (52.60%) were in the age group 26-35 years. 138 (27.60%) women were nulliparous and 282 (56.40%) were para 1-4 and anti-HCV and HBsAg were common in this parity group. Only 80 (16.00%) women were para 5 or more. All anti-HCV and HBsAg positive women were house-wives. Most of them were belonging to rural areas having poor socio-economic status. Among 35 anti-HCV positive women, 20 (57.14%) had history of previous surgery, while 13 (37.14%) had history of multiple injections, 5 (14.28%) received blood transfusion, 4 (11.42%) had ear/nose piercing while tattooing was seen in only 2 (5.71%). Among 23 HBsAg positive women, 10 (43.47%) had history of previous surgery. History of multiple injections was present in 6 (26.08%) patients, 4 (17.39%) patients had history of blood transfusion, tattooing, ear/nose piercing, history of dental

Membrana de poli (L-CO-D, L ÁCIDO LÁTICO-CO-TMC (PLDLA-TMC (70/30 com aloe vera para ser usado como curativo no tratamento de queimadura

Directory of Open Access Journals (Sweden)

Newton Maciel de Oliveira

2016-10-01

Full Text Available Ao longo da história, muitos tratamentos diferentes e, por vezes bizarros, têm sido prescritos para queimaduras. Desde os tempos mais remotos até os dias atuais, existe a necessidade de buscar novas terapias e drogas que funcionem como agentes tópicos para aliviar a dor e controlar a infecção em queimaduras. Dessa maneira, uma alternativa interessante às drogas sintéticas é a utilização de plantas, como um recurso natural, as quais podem ser úteis para o desenvolvimento de novas formulações e estratégias para melhorar a qualidade de vida. Neste trabalho foi utilizado o terpolímero poli (L-co-D, L ácido lático-co-TMC (PLDLA-co-TMC como matriz polimérica para a fabricação do curativo, sendo misturado a esse curativo o extrato de Aloe Vera, uma planta tropical que pertence à família Liliaceae, sendo conhecida por ser a mais antiga planta medicinal na natureza, a qual exibe propriedades anti-cancerígena, anti-oxidante, anti-bacteriana, cicatrizante e anti-viral. Além disso, outra vantagem do uso destes materiais está relacionada ao custo, uma vez que os produtos atualmente disponíveis para o tratamento de queimaduras são importados e apresentam valor elevado, inviabilizando o uso em larga escala. Neste trabalho, o estudo in vivo foi realizado por meio de 30 animais (ratos Wistar, divididos em 02 grupos conforme o tempo dos curativos (7 dias e 15 dias. Cada um, por sua vez, foi subdividido em 03 grupos de acordo com o tratamento aplicado: um grupo controle (sem a membrana PLDLA-co-TMC, tratamento 1 (com a membrana PLDLA-co-TMC sem Aloe Vera e tratamento 2 (com a membrana PLDLA-co-TMC/Aloe Vera com concentração de 10%.

Anticuerpos IgG anti-toxoplasma gondii en pacientes con síntomas atribuíbles a toxoplasmosis

Directory of Open Access Journals (Sweden)

Jorge E. Collazo

1993-12-01

Full Text Available Se evaluaron 6.520 pacientes con síntomas atribuíbles a toxoplasmosis por la presencia de anticuerpos IgG anti - Toxoplasma gondii mediante la técnica de inmunofluorescencia indirecta. El 51,27% de los evaluados resultaron positivos.El 60,85% de los pacientes con trastornos oculares son seropositivos a IgG anti - T. gondii; seguidos del 56,25 y 48,10% para aquellos con trastornos generales y gineco-obstétricos. Los síntomas o manifestaciones que evidenciaron mayor porcentaje de positividad fueron: astenia, coriorretinitis, trastornos menstruales, cefaleas y uveítis.

Hybrid mesons (Q anti Qg) in N anti N annihilation

International Nuclear Information System (INIS)

Dover, C.B.; Gutsche, T.; Faessler, A.

1993-09-01

N anti N annihilation reactions provide exciting possibilities to study mesonic resonances beyond the usual Q anti Q spectrum. Particularly the search for Q anti Qg mesons containing an explicit dynamical excitation of the gluon field is not promising, since hybrids are predicted to display unique features: exotic quantum numbers (J πC ) and dynamical selection rules for their decay modes. The authors have investigated the possibility of producing hybrids from p anti p atomic states in reactions of the type N anti N(L= 0,1) → π + Q anti Qg. Production rates for hybrid mesons are found to display a strong dependence on the quantum numbers and kinematical factors associated with the transition. The dependence on the orbital angular momentum L of the p anti p atomic state, accessible in p anti p annihilation at rest, would provide a striking signature for the production of hybrids. In estimating branching ratios for the formation of Q anti Qg hybrid mesons in N anti N annihilation reactions at rest, the authors have employed a microscopic model with constituent quarks and gluons in analogy to the annihilation model for the production of Q anti Q mesons

Secapin, a bee venom peptide, exhibits anti-fibrinolytic, anti-elastolytic, and anti-microbial activities.

Science.gov (United States)

Lee, Kwang Sik; Kim, Bo Yeon; Yoon, Hyung Joo; Choi, Yong Soo; Jin, Byung Rae

2016-10-01

Bee venom contains a variety of peptide constituents that have various biological, toxicological, and pharmacological actions. However, the biological actions of secapin, a venom peptide in bee venom, remain largely unknown. Here, we provide the evidence that Asiatic honeybee (Apis cerana) secapin (AcSecapin-1) exhibits anti-fibrinolytic, anti-elastolytic, and anti-microbial activities. The recombinant mature AcSecapin-1 peptide was expressed in baculovirus-infected insect cells. AcSecapin-1 functions as a serine protease inhibitor-like peptide that has inhibitory effects against plasmin, elastases, microbial serine proteases, trypsin, and chymotrypsin. Consistent with these functions, AcSecapin-1 inhibited the plasmin-mediated degradation of fibrin to fibrin degradation products, thus indicating the role of AcSecapin-1 as an anti-fibrinolytic agent. AcSecapin-1 also inhibited both human neutrophil and porcine pancreatic elastases. Furthermore, AcSecapin-1 bound to bacterial and fungal surfaces and exhibited anti-microbial activity against fungi and gram-positive and gram-negative bacteria. Taken together, our data demonstrated that the bee venom peptide secapin has multifunctional roles as an anti-fibrinolytic agent during fibrinolysis and an anti-microbial agent in the innate immune response. Copyright © 2016 Elsevier Ltd. All rights reserved.

Perfil lipídico e uso de anti-TNF-α

Directory of Open Access Journals (Sweden)

Antonio Carlos Ferraz Filho

2013-10-01

Full Text Available O uso do anti-TNF alfa tem sido associado a várias alterações no perfil lipídico, embora o estudo dessas alterações tenha gerado resultados que ainda são conflitantes. O conhecimento desse fato é de grande importância quando se observa a associação entre doenças reumáticas e aterogenêse acelerada. Esta pesquisa foi feita com o intuito de verificar alterações no perfil lipídico de usuários de anti-TNF-α na população do sul do Brasil e sua associação com tempo de uso, indicações, gênero do paciente e tipo de anti-TNF. Para tanto, analisaram-se os perfis de colesterol total (TC, HDL colesterol (HDLc, LDL colesterol (LDLc, índice aterogênico (IAT e triglicerídeos (TGs de 58 pacientes (42 com artrite reumatoide e 16 com espondiloartrites antes e depois do uso desse medicamento por um tempo mediano de 16,0 meses. Não se observaram alterações nos níveis de CT, HDLc, LDLc e IAT (P = NS. Todavia, houve um aumento significativo nos níveis de TGs (P = 0,03. A diferença mediana dos valores de TGs entre primeira e segunda medidas foi de 16 mg/dL, e esse aumento não estava associado ao gênero do paciente, tempo de uso, indicação de uso ou tipo de anti-TNF-α (P = NS. Concluiu-se que o uso de anti TNF-α está associado com aumento nos valores de TGs.

Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers Evaluación microbiológica de las diferentes formulaciones antisépticas, polivinil-pirrolidona-yodo y clorexidina, después de la contaminación intencional de los recipientes Avaliação microbiológica das diferentes formulações anti-sépticas, polivinilpirrolidona-iodo e clorexidina, após contaminação intencional das almotolias

Directory of Open Access Journals (Sweden)

Christiane Moreira Padovani

2008-12-01

Full Text Available This study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (PVP-I and chlorhexidine (CHX - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. A laboratory study was performed with 180 antiseptic containers, which were contaminated with Serratia marcescens [1 x 105 UFC/mL]. The containers were closed and stored, at room temperature, during seven days and shaken daily. The antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the non-disposable containers. These results suggested that antiseptic solutions inactivate microorganisms [1 x 105 UFC/mL]. Since cleaned antiseptic containers have around 102 UFC coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVP-I solutions in aqueous, detergent and alcoholic formulations.Los objetivos de este estudio fueron evaluar la sobre vivencia de los micro organismos en las diferentes formulaciones de los antisépticos clorexidina (CHX y polivinil-pirrolidona-yodo (PVP-Y, después de una contaminación intencional de los recipientes y extrapolar los resultados de los laboratorios para el cuidado mínimo a ser dispensado a los recipientes de múltiple uso para el envase de los antisépticos probados. Para esto, fue desarrollado un estudio de laboratorio, en que 180 recipientes fueron contaminados con 1 x 105 UFC/mL de suspensión conteniendo S.marcescens. Después de la contaminación, seis diferentes formulaciones de antisépticos (clorexidina y PVP-Y en los vehículos alcohólico, detergente y acuoso fueron distribuidos y sometidos a la cultura diaria durante siete días, a fin de verificar se hubo crecimiento del microorganismo. Los resultados de esta investigación permiten recomendar la limpieza como el procedimiento m

Técnicas anti-forenses para vídeos de dispositivos móviles

OpenAIRE

Quinto Huamán, Carlos

2016-01-01

En los últimos años el número de teléfonos móviles ha crecido notablemente. Producto de este impacto, los usuarios pasan gran parte de su tiempo sumergidos en este dispositivo. La cámara digital integrada en un teléfono inteligente es un componente usado frecuentemente para capturar gran cantidad de imágenes y vídeos de acontecimientos de la vida cotidiana de una persona. Esto permite dejar evidencia de situaciones que pueden comprometer la presunción de inocencia de las personas en actos que...

Configurações do ativismo anti-aids na contemporaneidade Anti-aids activism in the contemporaneity

Directory of Open Access Journals (Sweden)

Maio Spellman Quirino de Farias

2006-04-01

Full Text Available Este artigo tem como objetivo apresentar as investigações acerca da atual prática do ativismo anti-aids entre os técnicos vinculados ao Grupo de Apoio à Vida (GAV, na cidade de Campina Grande/PB. Outros objetivos da investigação foram: identificar as concepções de ativismo anti-aids para técnicos e conhecer como esses atores sociais avaliam essas práticas atualmente realizadas pela ONG. O favorecimento ou não da criação de novas estratégias de ativismo, através do funcionamento do GAV, também foi investigado. A pesquisa teve início com visitas à instituição, no segundo semestre de 2004, na cidade de Campina Grande-PB, onde foram realizadas entrevistas - a partir de um roteiro semi-estruturado - com seis técnicos da referida entidade. Resultados iniciais da pesquisa indicam que a nova forma de ativismo está consubstanciada na execução de projetos e na inserção da ONG nos conselhos de representação social, mas a mobilização de rua precisa ser resgatada.This article has the objective to present the investigations concerning the current practice of anti-aids activism among the technicians linked to the Group of Support to Life (GSL, in Campina Grande, Paraiba State - Brazil. Other objectives of the investigation were: to identify the conceptions of anti-aids activism for technicians and to know how they evaluate those practices accomplished by the Non-Governmental Organization. The favoring or not on the creation of new strategies of activism, through the operation of GSL, was also investigated. The research started with visits to the institution, in the second semester of 2004, in the city of Campina Grande, where semi-structured interviews were accomplished with six volunteer technicians of the referred entity. Preliminary results indicate that the new form of activism is consubstantiated in the execution of projects and in the insertion of NOG in the social representation councils, but the street mobilization needs

Papel del calcio en la modulación de la supervivencia intracelular de Mycobacterium tuberculosis

Directory of Open Access Journals (Sweden)

Luis Fernando García

2001-04-01

Full Text Available

Mycobacterium tuberculosis ( Mtb induce alteraciones en la señalización que podrían favorecer el crecimiento intracelular de la bacteria. Hay alteraciones en respuestas al IFNγ, activación de fosfatasas, inhibición del fagolisosoma y cambios en los flujos
de iones como el Ca2+. Estos eventos dependen de los receptores por los cuales sea reconocida la micobacteria, por ejemplo, en macrófagos murinos la interacción con el receptor de manosa (MMR, pero no con CD14, ni receptores tipo “scavenger” induce
flujos de Ca2+, que alteran la mitocondria y se asocian con apoptosis. En macrófagos humanos, la fagocitosis a través de receptores Fc, pero no de receptores de complemento, aumenta el Ca2+ que favorece la translocación al fagosoma de la calmodulina (CaM que activa la kinasa II dependiente de CaM (CaMKII, permitiendo la fusión del fagolisosoma y disminuyendo el crecimiento de Mtb. El bloqueo de esta vía favorece el crecimiento de la micobacteria. Esto indica que el Ca2+ puede modular la apoptosis y las señales que controlan la micobacteria, sugiriendo que la modulación de esta vía puede ser un blanco putativo para la intervención farmacológica. Por lo tanto proponemos como objetivo general establecer si la terapia anti-micobacteriana modula las vías de señalización dependientes de Ca2+ y la actividad de la CaMKII en macrófagos infectados con Mtb.

Hemolytic disease of the fetus and newborn caused by anti-D and anti-S alloantibodies: a case report

Directory of Open Access Journals (Sweden)

Yousuf Rabeya

2012-02-01

Full Text Available Abstract Introduction Hemolytic disease of the fetus and newborn is most commonly caused by anti-D alloantibody. It is usually seen in Rhesus D (RhD-negative mothers that have been previously sensitized. We report here a case of hemolytic disease of the fetus and newborn in a newborn baby caused by anti-D and anti-S alloantibodies, born to a mother who was RhD negative, but with no previous serological evidence of RhD alloimmunization. Case presentation A one-day-old Chinese baby boy was born to a mother who was group A RhD negative. The baby was jaundiced with hyperbilirubinemia, but with no evidence of infection. His blood group was group A RhD positive, his direct Coombs' test result was positive and red cell elution studies demonstrated the presence of anti-D and anti-S alloantibodies. Investigations performed on the maternal blood during the 22 weeks of gestation showed the presence of anti-S antibodies only. Repeat investigations performed post-natally showed the presence of similar antibodies as in the newborn and an anti-D titer of 1:32 (0.25 IU/mL, which was significant. A diagnosis of hemolytic disease of the fetus and newborn secondary to anti-D and anti-S was made. The baby was treated with phototherapy and close monitoring. He was discharged well after five days of phototherapy. Conclusions This case illustrates the possibility of an anamnestic response of allo-anti-D from previous sensitization in a RhD-negative mother, or the development of anti-D in mid-trimester. Thus, it highlights the importance of thorough antenatal ABO, RhD blood grouping and antibody screening, and if necessary, antibody identification and regular monitoring of antibody screening and antibody levels for prevention or early detection of hemolytic disease of the fetus and newborn, especially in cases of mothers with clinically significant red cell alloantibody.

Lipodystrophy syndrome associated with antiretroviral therapy in HIV patients: considerations for psychosocial aspects Síndrome de la lipodistrofia asociado con la terapia antiretroviral en pacientes con VIH: consideraciones para los aspectos psicosociales Sindrome da lipodistrofia associada com a terapia anti-retroviral em portadores do HIV: considerações para os aspectos psicossociais

Directory of Open Access Journals (Sweden)

Ana Paula Morais Fernandes

2007-10-01

son necesarios para entender mejor este complejo síndrome, proveyendo nueva información para ser utilizada en el cuidado de enfermería para pacientes con VIH que están afectados por este problema.Diversos efeitos colaterais têm sido associados à terapia anti-retroviral em portadores da infecção pelo HIV, dentre esses, a síndrome da lipodistrofia apresentando hiperlipidemia e alterações na forma do corpo, com hipertrofia adiposa central e lipoatrofia periférica, relatada pelos pacientes como um visível marcador para a identificação de portadores da infecção pelo HIV. Este estudo consiste em análise da produção científica sobre aspectos psicossociais em portadores da infecção pelo HIV que apresentam lipodistrofia associada à utilização da terapia anti-retroviral. Os resultados mostram que alterações corporais podem ser suficientemente perturbadoras para o bem-estar psicossocial, afetando a qualidade de vida e aumentando o estigma da doença, ocasionando perturbações nas relações sociais. Esta revisão possibilita uma análise preliminar dos aspectos psicossocias da lipodistrofia; entretanto, outros estudos são necessários para o melhor entendimento desta complexa síndrome, trazendo novas informações a serem utilizadas no cuidado de enfermagem a portadores da infecção pelo HIV afetados por este problema.

Estudo da Adesão à Quimioprofilaxia Anti-retroviral para a Infecção por HIV em Mulheres Sexualmente Vitimadas Study of Adherence to Antiretroviral Chemoprophylaxis for HIV Infection in Sexually Abused Women

Directory of Open Access Journals (Sweden)

Jefferson Drezett

1999-10-01

Full Text Available Objetivos: embora não existam dados apropriados para estabelecer sua eficácia, alguns serviços tem utilizado, profilaticamente, a terapia anti-retroviral para o HIV nos casos de violência sexual. O objetivo deste estudo foi avaliar a aceitabilidade, tolerância e adesão a um esquema quimioprofilático para o HIV. Pacientes e método: foram avaliadas 62 mulheres vítimas de estupro e/ou atentado violento ao pudor com coito ectópico anal. Os agressores foram referidos como desconhecidos. A profilaxia foi iniciada dentro das primeiras 48 h da violência e mantida por 4 semanas, sendo administrados diariamente: zidovudina, 600 mg; indinavir, 2.400 mg e lamivudina, 300 mg. Resultados: a taxa de descontinuidade foi de 24,2%, sendo em 12 casos (80% decorrente de intolerância gástrica. Os efeitos colaterais estiveram presentes em 43 casos (69,4%, sendo as náuseas e vômitos os mais freqüentes. A complexidade posológica e o tempo de uso foram fatores possivelmente associados ao uso inadequado das drogas, ocorrendo em 10,6% dos casos. Conclusão: a taxa de descontinuidade da quimioprofilaxia foi semelhante à observada em outras indicações.Purpose: some medical institutions have been prophylactically ministrating anti-HIV therapy in cases of sexual violence, although there are no appropriate basic facts to establish its efficacy. The aim of the present study was to evaluate the acceptance, tolerance and adhesion of these women under a chemoprophylaxis plan for HIV. Methods: sixty-two women victims of rape and/or anal intercourse with unknown aggressors have been evaluated. Prophylaxis has been started within the first 48 h after violence and maintained for 4 weeks, with daily administration of zidovudine, 600 mg; indinavir, 2,400 mg and lamivudine, 300 mg. Results: the discontinuance rate was 24.2%, withe 12 cases (80% due to gastric intolerance. The side effects were present in 43 cases (69.4%, including nausea and vomitting as the most

Anti-Diabetic, Anti-Oxidant and Anti-Hyperlipidemic Activities of Flavonoids from Corn Silk on STZ-Induced Diabetic Mice.

Science.gov (United States)

Zhang, Yan; Wu, Liying; Ma, Zhongsu; Cheng, Jia; Liu, Jingbo

2015-12-23

Corn silk is a well-known ingredient frequently used in traditional Chinese herbal medicines. This study was designed to evaluate the anti-diabetic, anti-oxidant and anti-hyperlipidemic activities of crude flavonoids extracted from corn silk (CSFs) on streptozotocin (STZ)-induced diabetic mice. The results revealed that treatment with 300 mg/kg or 500 mg/kg of CSFs significantly reduced the body weight loss, water consumption, and especially the blood glucose (BG) concentration of diabetic mice, which indicated their potential anti-diabetic activities. Serum total superoxide dismutase (SOD) and malondialdehyde (MDA) assays were also performed to evaluate the anti-oxidant effects. Besides, several serum lipid values including total cholesterol (TC), triacylglycerol (TG), low density lipoprotein cholesterol (LDL-C) were reduced and the high density lipoprotein cholesterol level (HDL-C) was increased. The anti-diabetic, anti-oxidant and anti-hyperlipidemic effect of the CSFs suggest a potential therapeutic treatment for diabetic conditions.

anti p and anti Λ production in Si+Au collisions at the AGS

International Nuclear Information System (INIS)

Wu, Yuedong

1996-01-01

Anti p and anti Λ production in central Si + Au collisions has been measured by E589 at the BNL-AGS. Preliminary m perpendicular spectra are presented for anti p's and anti Λ's. The dn/dy distribution for anti p's is also presented. Based on the anti p and anti Λ measurements, anti Λ/anti p ratios are calculated in the rapidity range of 1.1-1.5

Estado actual y futuro de la terapia anti-leishmaniásica en Colombia

Directory of Open Access Journals (Sweden)

Jaime Soto

2006-10-01

Full Text Available Los antimoniales pentavalentes son la primera elección para el tratamiento de la leishmaniasis en Colombia pero la dosis ha tenido que incrementarse en mas de 600% para mantener su eficacia que en distintos reportes varía entre 93 y 25%, lo cual puede reflejar diferencias en la sensibilidad de los parásitos pero más probablemente incumplimiento de los esquemas recomendados. Los resultados de su empleo, así como la situación actual de pentamidina, anfotericina B y miltefosina son revisados y se hace una proyección sobre el desarrollo de la terapia en los próximos años. Para definir el real estado de la respuesta a los medicamentos anti-leishmaniásicos y prolongarles su vida útil, es indispensable establecer mecanismos de vigilancia de los factores implicados en el manejo de la enfermedad por lo que se plantea una estrategia de sitios centinela que permitan observaciones puntuales en el tiempo, en áreas específicas y sobre situaciones particulares.

Los óxidos de Fe-Ti de las rocas calco-alcalinas del sureste de España

Directory of Open Access Journals (Sweden)

López Ruiz, J.

1984-12-01

Full Text Available The Fe-Ti oxides appearing in the calc-alkaline rocks from SE Spain are titanomagnetite and ilmenite, although there are also sorne crystals of maghemite resulting from the oxidation of the rnagnetite. Titanomagnetite is to be found in all petrologic types, but the second is absent from the basaltic andesites and even from the less siliceous andesites. The titanomagnetites present a low proportion of molecule ulvospinel (10.6-28.9%, because of the low content in TiO₂ of these lavas . The percentage of R.O. in the ilmenites analysed is relatively small (19.7-26.5%. However, both oxides are progressively richer in the above-mentioned molecules as they pass from andesites to dacites . From the chemical composition of both coexisting oxide phases it has been estimated, using the curves of Buddington and Lidsley (1964, that the andesites crystallized at temperatures between 880°C and 855°C and under oxygen fugacities of around 10^-11.2 and 10^-11.4 atm., while the temperatures in the case of the dacites were between 880°C and 8l0°C, with oxygen fugacities of 10^-11.4 and 10^-12.3 atm. The fO₂-T curve of these rocks is therefore situated above that of nickel-nickel oxide (NNO buffer. These crystallization temperatures are somewhat lower than those determined by using the pair orthopyroxene-c1inopyroxene (Wood and Banno, 1973 and Wells, 1977. However, since it is widely considered that the titanomagnetite-ilmenite geothermometer is the most accurate, these temperatures are considered more correct than those determined from the composition of pyroxenes .

Los óxidos de Fe-Ti que aparecen en las rocas calco-alcalinas del sureste de España son titanomagnetita e ilmenita, si bien también hay algunos cristales de maghemita, producto de la oxidación de la magnetita. La primera está presente en todos los tipos petrológicos existentes , pero la segunda está ausente en las andesitas

Anti-Diabetic, Anti-Oxidant and Anti-Hyperlipidemic Activities of Flavonoids from Corn Silk on STZ-Induced Diabetic Mice

Directory of Open Access Journals (Sweden)

Yan Zhang

2015-12-01

Full Text Available Corn silk is a well-known ingredient frequently used in traditional Chinese herbal medicines. This study was designed to evaluate the anti-diabetic, anti-oxidant and anti-hyperlipidemic activities of crude flavonoids extracted from corn silk (CSFs on streptozotocin (STZ-induced diabetic mice. The results revealed that treatment with 300 mg/kg or 500 mg/kg of CSFs significantly reduced the body weight loss, water consumption, and especially the blood glucose (BG concentration of diabetic mice, which indicated their potential anti-diabetic activities. Serum total superoxide dismutase (SOD and malondialdehyde (MDA assays were also performed to evaluate the anti-oxidant effects. Besides, several serum lipid values including total cholesterol (TC, triacylglycerol (TG, low density lipoprotein cholesterol (LDL-C were reduced and the high density lipoprotein cholesterol level (HDL-C was increased. The anti-diabetic, anti-oxidant and anti-hyperlipidemic effect of the CSFs suggest a potential therapeutic treatment for diabetic conditions.

SITUASI PATEN OBAT ANTI DIABETES, ANTI HIPERTENSI, ANTI MALARIA DAN ANTI TUBERKULOSIS DI INDONESIA

Directory of Open Access Journals (Sweden)

Basundari Sri Utami

2014-10-01

Full Text Available AbstrakIndonesia merupakan negara berpenduduk keempat terbanyak setelah Cina, India dan Amerika. Indonesia sedang mengalami transisi epidemiologi, dimana terjadi peningkatan penyakit tidak menular (PTM, sementara penyakit menular (PM seperti malaria, tuberkulosis dan demam dengue prevalensinya masih tinggi. Tingginya morbiditas merupakan lahan yang bagus untuk melaksanakan obat anti PM dan anti PTM yang mendapat paten karena pangsa pasarnya yang sangat luas. Sayangnya potensi pasar yang masih luas ini hanya ditangkap oleh luar negeri. Data dari Direktorat Jendral Hak Kekayaan Intelektual (Ditjen HKI pada tahun 2010 menunjukkan pemohon paten dalam negeri yang mendapatkan persetujuan perlindungan paten (granted hanya 4,6% sedangkan dari luar negeri sebanyak 92,03%. Hal yang sangat ironis bagi Indonesia yang merupakan negara dengan potensi bahan dasar obat alam dan keanekaragaman hayati terbanyak ketiga setelah Brazil dan Cina. Tujuan penelitian ini untuk mengevaluasi situasi paten obat yang terdaftar di Direktorat Paten, Ditjen HKI, Kementerian Hukum dan Hak Asasi Manusia RI dalam 7 tahun terakhir (tahun 2005 sampai 2011 untuk PM (malaria dan tuberkulosis dan PTM (hipertensi dan diabetes. Metode observasional dengan penelusuran dokumen paten dari alamat web instansi terkait. Hasilnya Indonesia hanya mendaftarkan 4,9% dari seluruh paten yang didaftarkan di Dirjen HKI dari tahun 2005 sampai dengan 2011, sebagai berikut untuk obat anti-hipertensi 3,4% dari 89 paten, anti-diabetes hanya 4,8% dari 250 paten, anti malaria 21,1% dari 18 paten anti-tuberkulosis 7,1% dari 14 paten. Sebagian besar paten yang didaftarkan oleh pendaftar Indonesia merupakan paten obat ekstrak herbal atau komposisinya. Kesimpulan dari penelitian ini adalah paten obat untuk PTM dan PM di Indonesia masih didominasi paten luar negeri.Kata Kunci : Situasi paten, obat, Ditjen HKI, IndonesiaAbstractIndonesia is the fourth most populous country after China, India and America. There

Detecção de imunoglobulinas IgG, IgM e IgA anti-Toxoplasma gondii no soro, líquor e saliva de pacientes com síndrome da imunodeficiência adquirida e neurotoxoplasmose Detection of anti-Toxoplasma gondii IgG, IgM and IgA immunoglobulins in the serum, cerebrospinal fluid and saliva of patients with acquired immunodeficiency syndrome and neurotoxoplasmosis

Directory of Open Access Journals (Sweden)

Aercio Sebastião Borges

2004-12-01

Full Text Available Estudamos 55 pacientes com sindrome da imunodeficiência adquirida (SIDA e neurotoxoplasmose (grupo 1; 37 pacientes com SIDA e comprometimento neurológico por outra etiologia (grupo 2 e 18 indivíduos anti-HIV negativos com manifestações neurológicas (grupo 3, pesquisando IgG, IgA e IgM anti-Toxoplasma gondii, no soro, líquor e saliva, utilizando teste ELISA, para fins diagnósticos. O valor preditivo negativo do teste para o encontro de IgG no soro foi 100% e no líquor, 92,4%. Não houve diferença entre os três grupos quanto aos anticorpos IgA neste material. Para IgA, no líquor, o teste alcançou 72,7% de especificidade (pWe studied 55 patients with acquired immunodeficiency syndrome (AIDS and neurotoxoplasmosis (group 1, 37 patients with AIDS and neurological involvement due to another etiology (group 2 and 18 anti-HIV-negative individuals with neurological manifestations, by searching for anti-T. gondii IgG, IgA and IgM immunoglobulins in serum, cerebrospinal fluid (CSFand saliva, using ELISA. The negative predictive value of the test for IgG in serum was 100% and in CSF, 92.4%. There was no difference among the three groups studied regarding IgA in serum. For IgA, in CSF the test reached 72.7% specificity (p<0.05. In saliva, only the detection of IgG was found to be correlated with a diagnosis of neurotoxoplasmosis. We emphasize that the absence of anti-T. gondii IgG antibodies in serum and CSF strongly indicates the absence of a diagnosis of neurotoxoplasmosis and that specific IgA immunoglobulins in CSF and IgG in saliva may represent two auxiliary markers for the differential diagnosis of toxoplasmic encephalitis in AIDS.

Anti-Ma and anti-Ma2-associated paraneoplastic neurological syndromes.

Science.gov (United States)

Ortega Suero, G; Sola-Valls, N; Escudero, D; Saiz, A; Graus, F

Analyse the clinical profile, associated tumour types, and response to treatment of paraneoplastic neurological syndromes associated with antibodies against Ma proteins. A retrospective study of patients with antibodies against Ma proteins identified in a neuroimmunology laboratory of reference. Of the 32 patients identified, 20 showed reactivity against Ma2 only (anti-Ma2 antibodies), 11 against Ma1 and Ma2 (anti-Ma antibodies), and 1 with reactivity against Ma1 only (anti-Ma1 antibodies). The most common clinical presentations were limbic encephalopathy, diencephalic dysfunction, or brainstem encephalopathy, frequently appearing as a combination of these features. Three patients had isolated cerebellar dysfunction with anti-Ma antibodies, and 2 exhibited peripheral nervous system syndrome with anti-Ma2 antibodies. Testicular tumours were the most common neoplasms (40%) in the anti-Ma2 cases. In the group associated with anti-Ma1 antibodies, the most common were lung tumours (36%), followed by testicular tumours. All idiopathic cases were reactive to Ma2. The clinical outcome was significantly better in the anti-Ma2 group. The patient with anti-Ma1 presented with limbic encephalitis and brainstem dysfunction associated with lymphoepithelioma of the bladder. Specifically determining the different reactivities of anti-Ma protein antibodies in order to differentiate between Ma1 and Ma2 antibodies is important because anti-Ma2-associated paraneoplastic syndromes have a better outcome. Lastly, this study is the first to confirm that there may be cases that react exclusively to antibodies against Ma1. Copyright © 2016 Sociedad Española de Neurología. Publicado por Elsevier España, S.L.U. All rights reserved.

La problemática de la epistemología anti-suerte

Directory of Open Access Journals (Sweden)

Abel Wajnerman Paz

2017-01-01

Full Text Available D. Pritchard ha sostenido que el conocimiento requiere la satisfacción de una condición de habilidad y una anti-suerte que no guardan relación de implicación entre sí. Se sostiene que la satisfacción de una condición anti-suerte implica cumplir con la condición de habilidad, primero, porque, las características centrales del caso de D. Pritchard (TEMP en contra de esta implicación son compartidas con casos en los que hay habilidad; y segundo, el caso de A. Goldman del dios benevolente es más efectivo que TEMP, pero involucra un tipo de seguridad diferente a la requerida para el conocimiento. Por último, se muestra que la respuesta de D. Pritchard al problema de la generalidad bloquea la implicación de seguridad a habilidad. Se propone un contraejemplo, adaptando un caso tomado de la epistemología del testimonio.

Hemolytic disease of the newborn due to anti-U Doença hemolítica do recém-nascido devido a anti-U

Directory of Open Access Journals (Sweden)

Marcia Cristina Zago Novaretti

2003-01-01

Full Text Available Anti-U is a rare red blood cell alloantibody that has been found exclusively in blacks. It can cause hemolytic disease of the newborn and hemolytic transfusion reactions. We describe the case of a female newborn presenting a strongly positive direct antiglobulin test due to an IgG antibody in cord blood. Anti-U was recovered from cord blood using acid eluate technique. Her mother presented positive screening of antibodies with anti-U identified at delivery. It was of IgG1 and IgG3 subclasses and showed a titer of 32. Monocyte monolayer assay showed moderate interaction of Fc receptors with maternal serum with a positive result (3.1%. The newborn was treated only with 48 hours of phototherapy for mild hemolytic disease. She recovered well and was discharged on the 4th day of life. We conclude that whenever an antibody against a high frequency erythrocyte antigen is identified in brown and black pregnant women, anti-U must be investigated.Anti-U é um aloanticorpo eritrocitário raro detectado exclusivamente em negros, que pode causar doença hemolítica do recém-nascido e reações transfusionais hemolíticas. Relatamos o caso de um recém-nascido, de sexo feminino, que apresentou teste de antiglobulina direto fortemente positivo, dirigido a um anticorpo IgG em sangue de cordão umbilical. Anti-U foi identificado por técnica de eluição ácida. A mãe apresentava pesquisa de anticorpos irregulares positiva com anticorpo anti-U, de subclasses IgG1 e IgG3, título 32, identificado ao nascimento. O ensaio de monocamada de monócitos apresentou resultado positivo (3.1%, mostrando uma interação moderada de receptores Fc com soro materno. O recém-nascido foi tratado somente por fototerapia durante 48 horas para uma doença hemolítica leve. A criança recuperou-se bem e teve alta médica no quarto dia de vida. Concluímos que quando um anticorpo contra um antígeno eritrocitário de alta freqüência for identificado em gestantes negras e pardas

Intolerância glicêmica e o prognóstico perinatal em gestantes utilizando anti-retrovirais

Directory of Open Access Journals (Sweden)

El Beitune Patrícia

2003-01-01

Full Text Available OBJETIVOS: estudar o efeito das drogas anti-retrovirais sobre o metabolismo glicêmico em gestantes portadoras do HIV-1 e a ação dessas medicações sobre o prognóstico perinatal. MÉTODOS: estudo prospectivo realizado em 57 gestantes divididas em três grupos: grupo AZT, utilizando zidovudina (n=20; grupo TT, utilizando zidovudina+lamivudina+nelfinavir (n=25, e grupo controle, gestantes normais (n=12. Obteve-se a área sob a curva (ASC das glicemias após teste oral de tolerância à glicose com 75 g de glicose em quatro oportunidades durante a gravidez (1º=14-20 semanas, 2º=21-26 semanas, 3º=27-32 semanas e 4º=33-38 semanas. O prognóstico perinatal levou em consideração as taxas de prematuridade, restrição de crescimento intra-útero (RCIU, baixo peso ao nascer, mortalidade perinatal e transmissão vertical do HIV-1. Os dados foram analisados utilizando-se os testes não paramétricos do c², de Friedman e de Kruskal-Wallis. RESULTADOS: os valores da mediana da ASC foi de 11.685 mg/dL-2h para o grupo controle, 13.477 mg/dL-2h para o grupo AZT e 13.650 mg/dL-2h para o grupo TT (p=0,049. Não se observou efeito deletério dos anti-retrovirais sobre as taxas de prematuridade, baixo peso ao nascer, RCIU e índices de Apgar. Não houve nenhum caso de transmissão vertical do HIV-1. CONCLUSÕES: verificou-se o desenvolvimento de intolerância glicêmica em gestantes que utilizaram tratamento tríplice, não sendo observado naquelas que utilizaram apenas AZT. Não houve efeitos deletérios dos anti-retrovirais sobre o prognóstico perinatal.

Iodine-131 labeled anti-CEA polyclonal antibody detection of gastrointestinal cancer

International Nuclear Information System (INIS)

Nabi, H.A.; Hinkle, G.H.; Olsen, J.O.; Haagensen, D.A.; Thurston, M.O.; Mojzisik, C.; Houchens, D.; Martin, E.W. Jr.

1984-01-01

To localize gastrointestinal tumor, 31 patients were injected with 1.7-2.1 mCi I-131 anti-CEA baboon polyclonal antibody. Whole body imaging at 48, 72, and occasionally 96 hrs was performed with a Signa Camera (Technicare) peaked at 364 keV with 20% window. Additional spot views were usually obtained. No subtraction methods were used. All patients had surgical and pathological confirmation of the nuclear medicine studies. Labeled antibody images were positive in 15 (8 recurrent or metastatic colorectal, 2 gastric, 1 pancreatic, 1 primary colon, and 1 breast metastatic to chest wall). In 1, antibody images were positive for metastatic deposits in para-aortic lymph nodes, but negative for primary rectal tumor. True negative images were observed in 6; false negative images in 9 (4 liver metastases, 2 rectal, 1 pancreatic, 1 mesenteric lymph node metastasis, 1 bone metastasis). In all cases, no correlation existed between preoperative CEA serum levels and imaging. I-131 labeled anti-CEA polyclonal antibody imaging proved highly efficient in detecting gastric cancer (2/2) and moderately efficient in detecting recurrent colorectal cancer (8/15). On the other hand, the I-131 labeled polyclonal anti-CEA antibody imaging was of limited value in detecting colon cancer (1/9), pancreatic cancer (1/4) and metastatic liver disease

Exotic tetraquark states with the qq anti Q anti Q configuration

Energy Technology Data Exchange (ETDEWEB)

Luo, Si-Qiang; Chen, Kan; Liu, Xiang [Lanzhou University, School of Physical Science and Technology, Lanzhou (China); Lanzhou University and Institute of Modern Physics, CAS, Research Center for Hadron and CSR Physics, Lanzhou (China); Liu, Yan-Rui [Shandong University, School of Physics and Key Laboratory of Particle Physics and Particle Irradiation (MOE), Jinan (China); Zhu, Shi-Lin [Peking University, School of Physics and State Key Laboratory of Nuclear Physics and Technology, Beijing (China); Collaborative Innovation Center of Quantum Matter, Beijing (China); Peking University, Center for High Energy Physics, Beijing (China)

2017-10-15

In this work, we study systematically the mass splittings of the qq anti Q anti Q (q = u, d, s and Q = c, b) tetraquark states with the color-magnetic interaction by considering color mixing effects and estimate roughly their masses. We find that the color mixing effect is relatively important for the J{sup P} = 0{sup +} states and possible stable tetraquarks exist in the nn anti Q anti Q (n = u, d) and ns anti Q anti Q systems either with J = 0 or with J = 1. Possible decay patterns of the tetraquarks are briefly discussed. (orig.)

[Anti-inflammatory, analgesic and anti-pyretic activities of a non-steroidal anti-inflammatory drug, etofenamate, in experimental animals].

Science.gov (United States)

Nakamura, H; Motoyoshi, S; Imazu, C; Ishii, K; Yokoyama, Y; Seto, Y; Kadokawa, T; Shimizu, M

1982-08-01

Anti-inflammatory, analgesic, and anti-pyretic activities of orally administered etofenamate, the diethylene glycol ester of flufenamic acid, were investigated in experimental animals. Against acetic acid-induced vascular permeability in mice and ultra-violet light-induced erythema in guinea pigs, etofenamate produced a dose related inhibition at doses of 40--320 mg/kg and 5--20 mg/kg, respectively. In rats, felt-pellet-induced granuloma formation and adjuvant-induced arthritis were significantly inhibited by repeated administration of etofenamate at doses of 20 mg/kg/day for 5 days and 40 mg/kg/day for 21 days, respectively. Etofenamate showed an inhibitory activity on the squeak response caused by flexing and extending the silver nitrate-induced arthritic joint in rats; and it produced a dose related anti-writhing activity at doses of 50--300 mg/kg and 10--80 mg/kg in mice and rats, respectively, in the acetic acid-induced writhing test. Etofenamate showed a significant anti-pyretic activity at doses of 0.2 mg/kg or more. These potencies of etofenamate were 0.5 to 1.6 times those of flufenamic acid. In particular, the anti-erythema, anti-arthritis, and anti-pyretic activities of etofenamate were approximately equivalent to or superior to those of flufenamic acid. From these results, it was suggested that etofenamate given orally, like other non-steroidal anti-inflammatory drugs, showed anti-inflammatory, analgesic, and anti-pyretic activities in experimental animals.

C anti c q anti q states

Energy Technology Data Exchange (ETDEWEB)

Chao, K T [Oxford Univ. (UK). Dept. of Theoretical Physics; Science Research Council, Chilton (UK). Rutherford and Appleton Labs.)

1980-07-01

Taking account of the colour magnetic and electric forces, we discuss the spectroscopy of various types of c anti c q anti q states. Their decay, hadronic production, production in e/sup +/e/sup -/ annihilation as well as photoproduction are also studied.

Aglutinina anti-T (Thomsen-Friedenreich na doença de Chagas

Directory of Open Access Journals (Sweden)

Marcos Palatnik

1986-10-01

Full Text Available Uma amostra de soros de pacientes e controles de uma área endêmica da Doença de Chagas do País foi titulada contra hemácias de grupo O tratadas pela neuraminidase (células OT ativadas e hemacias normais de grupo A e B (sistema ABO. Também foram feitos alguns testes de aglutinação de hemácias de pacientes com cardiomiopatia com as lectinas de amendoim (anti-T + anti-Tk e com anticorpo humano anti-T. O escore médio da aglutinina anti-T dos pacientes foi similar ao do grupo controle. En- tretanto, o escore médio dos indivíduos com a forma cardíaca íoi significantemente maior que o dos pacientes assintomáticos e também maior que o do grupo controle. A comparação entre indivíduos com sorologia diagnóstica positiva versus negativa foi não significante. Nem a sorologia para Chagas nem as variáveis raça, sexo, idade, idade ao quadrado, in-fecção intestinal parasitária, teor de proteínas do soro, volume globular eritrocitário (hema-tócrito, grau de palidez da mucosa bucal, cardíomiopatia e os termos de interação sexo x idade, sexo x idade ao quadrado, estão associados significativamente com o logaritmo do título ou com o logaritmo do escore de aglutinação. Os escores de aglutinação das aglutininas anti-A e anti-B do sistema de grupo sangüíneo ABO estão dentro da faixa de variação normal. As hemácias de pacientes com a forma cardíaca não são aglutinadas nem pelas lectinas de amendoim (antiT + anti-Tk e da soja (anti-T nem pela aglutinina anti-T de origem humana. A prova de Coombs direta foi negativa em todos os pacientes, demonstrando a aparente ausência de imunoglobulinas IgG e IgM na superfície eritrocitária. O fato de que o aumento dos níveis de anti-T só foi detectado em uma das formas clínicas da doença em pacientes da mesma área endêmica constitui um argumento contra a possibilidade de que a variação observada seja produzida por uma infecção concomitante.

Poly herbal formulation with anti-elastase and anti-oxidant properties for skin anti-aging.

Science.gov (United States)

Kalyana Sundaram, Induja; Sarangi, Deepika Deeptirekha; Sundararajan, Vignesh; George, Shinomol; Sheik Mohideen, Sahabudeen

2018-01-29

Skin forms an important part of human innate immune system. Wrinkles, thinning and roughening of skin are some of the symptoms that affect the skin as it ages. Reactive oxygen species induced oxidative stress plays a major role in skin aging by modulating the elastase enzyme level in the skin. Extrinsic factors that affect skin aging such as UV radiation can also cause malignant melanoma. Here we selected four medicinal plant materials, namely, leaves of Nyctanthes arbor-tristis, unripe and ripe Aegle marmelos fruit pulp and the terminal meristem of Musa paradisiaca flower and investigated their anti-aging properties and cytotoxicity in vitro individually as well as in a poly herbal formulation containing the four plant extracts in different ratios. The phytochemical contents of the plant extracts were investigated for radical scavenging activity and total reducing power. Based upon its anti-oxidant properties, a poly herbal formulation containing leaves of Nyctanthes arbor-tristis, unripe and ripe fruit pulp of Aegle marmelos, and the terminal meristem of Musa paradisiaca flower in the ratio 6:2:1:1 (Poly Herbal Formulation 1) and 1:1:1:1 (Poly Herbal Formulation 2), respectively were formulated. It has been observed that the Poly Herbal Formulation 1 was more potent than Poly Herbal Formulation 2 due to better anti-oxidant and anti-elastase activities in NIH3T3 fibroblast cells. In addition Poly Herbal formulation 1 also had better anti-cancer activity in human malignant melanoma cells. Based on these results these beneficial plant extracts were identified for its potential application as an anti-aging agent in skin creams as well as an anti-proliferation compound against cancer cells.

Study of phytochemical, anti-microbial, anti-oxidant, and anti-cancer properties of Allium wallichii.

Science.gov (United States)

Bhandari, Jaya; Muhammad, BushraTaj; Thapa, Pratiksha; Shrestha, Bhupal Govinda

2017-02-08

There is growing interest in the use of plants for the treatment and prevention of cancer. Medicinal plants are currently being evaluated as source of promising anticancer agents. In this paper, we have investigated the anticancer potential of plant Allium wallichii, a plant native to Nepal and growing at elevations of 2300-4800 m. This is the first study of its kind for the plant mentioned. The dried plant was extracted in aqueous ethanol. Phytochemical screening, anti-microbial assay, anti-oxidant assay, cytotoxicity assay and the flow-cytometric analysis were done for analyzing different phytochemicals present, anti-microbial activity, anti-oxidant activity and anti-cancer properties of Allium wallichii. We observed the presence of steroids, terpenoids, flavonoids, reducing sugars and glycosides in the plant extract and the plant showed moderate anti-microbial and anti-oxidant activity. The IC 50 values of Allium wallichii in different cancer cell lines are 69.69 μg/ml for Prostate cancer (PC3) cell line, 55.29 μg/ml for Breast Cancer (MCF-7) cell line and 46.51 μg/ml for cervical cancer (HeLa) cell line as compared to Doxorubicin (0.85 μg/ml). The cell viability assay using FACS showed that the IC 50 value of Allium wallichii for Burkitt's lymphoma (B-Lymphoma) cell line was 3.817 ± 1.99 mg/ml. Allium wallichii can be an important candidate to be used as an anticancer agent. Separation of pure compounds with bioassay guided extraction, spectrometric analysis and subsequent cytotoxicity assay of the pure bioactive compounds from Allium wallichii is highly recommended as the crude extract itself showed promising cytotoxicity.

Anti-neuronal anti-bodies in patients with early psychosis.

Science.gov (United States)

Mantere, O; Saarela, M; Kieseppä, T; Raij, T; Mäntylä, T; Lindgren, M; Rikandi, E; Stoecker, W; Teegen, B; Suvisaari, J

2018-02-01

It may be challenging to distinguish autoimmune encephalitis associated with anti-neuronal autoantibodies from primary psychiatric disorders. Here, serum was drawn from patients with a first-episode psychosis (n=70) or a clinical high-risk for psychosis (n=6) and controls (n=34). We investigated the serum prevalence of 24 anti-neuronal autoantibodies: IgG antibodies for anti-N-methyl-d-aspartate-type glutamate receptor (anti-NMDAR), glutamate and γ-aminobutyric acid alpha and beta receptors (GABA-a, GABA-b), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPA), glycine receptor (GlyR), metabotropic glutamate receptor 1 and 5 (mGluR1, mGluR5), anti-Tr/Delta/notch-like epidermal growth factor-related receptor (DNER), contactin-associated protein-like 2 (CASPR2), myelin oligodendrocyte glycoprotein (MOG), glutamic acid decarboxylase-65 (GAD65), collapsin response mediator protein 5/crossveinless-2 (CV2), aquaporin-4 (AQP4), anti-dipeptidyl-peptidase-like protein-6 (DPPX), type 1 anti-neuronal nuclear antibody (ANNA-1, Hu), Ri, Yo, IgLON5, Ma2, zinc finger protein 4 (ZIC4), Rho GTPase-activating protein 26, amphiphysin, and recoverin, as well as IgA and IgM for dopamine-2-receptor (DRD2). Anti-NMDA IgG antibodies were positive with serum titer 1:320 in one patient with a clinical high risk for psychosis. He did not receive a diagnosis of encephalitis after comprehensive neurological evaluation. All other antineuronal autoantibodies were negative and there were no additional findings with immunohistochemistry of brain issues. Copyright © 2017 Elsevier B.V. All rights reserved.

Análise sistemática da influência do antifator de necrose tumoral [anti-TNF] sobre as taxas de infecção em pacientes com artrite reumatoide

Directory of Open Access Journals (Sweden)

Leslie Goh

2013-12-01

Full Text Available OBJETIVO: O presente trabalho tem como objetivo fornecer uma análise sistemática da influência do anti-TNF sobre as taxas de infecção em pacientes com artrite reumatoide (AR. MÉTODO: Pesquisamos na Medline para obter informações de controle de qualidade sobre as taxas de infecção em pacientes com AR tratados com anti-TNF. RESULTADOS: Atualmente, uma proporção elevada de pacientes com AR é usuária de agentes anti-TNF. Dados de registros nacionais em países da Europa de pacientes com AR tratados com anti-TNF sugerem que terapias biológicas estão intimamente ligadas à sepse. Apesar de estudos anteriores terem relatado um maior risco de infecções, atualmente há dados emergentes com maior duração de acompanhamento que sugerem um risco ajustado de 1,2. Os pacientes idosos e os com doença de longa data poderão apresentar uma taxa mais elevada de infecções graves em comparação às suas contrapartes mais novas com doença inicial. Hoje, há dados emergentes que sugerem que a terapia com anti-TNF está associada ao desenvolvimento de neutropenia logo após o início do tratamento. Os registros biológicos constataram que os pacientes com ARES tratados com anticorpos monoclonais apresentam aumento no risco de tuberculose (TB, em comparação aos tratados com bloqueadores dos receptores de TNF. Esse risco de infecção precisa ser ponderado em relação aos benefícios estabelecidos dos bloqueadores de TNF. CONCLUSÃO: A evidência atual sugere que o tratamento com anti-TNF na AR está intimamente associado à infecção. Os pacientes precisam estar cientes do risco de infecção, assim como dos benefícios estabelecidos dos bloqueadores de TNF, para que possam fornecer o consentimento informado para o tratamento.

¿Es necesario investigar anticuerpos IgM contra el virus de la hepatitis A cuando el enzimograma hepático es normal? Is it necessary to investigate anti-hepatitis A virus (HAV IgM antibodies when the hepatic enzymogram is normal?

Directory of Open Access Journals (Sweden)

C. Coitinho

2007-09-01

Full Text Available La hepatitis por virus A (VHA es la más frecuente de las hepatitis virales en el mundo, especialmente en los países subdesarrollados. Donde esta enfermedad es endémica, se suelen realizar un gran número de estudios de laboratorio para confirmarla. El objetivo del presente trabajo fue evaluar la utilidad de investigar anticuerpos IgM anti hepatitis A (IgM anti-VHA para el diagnóstico de la VHA en pacientes con niveles séricos normales de aspartato y alanina aminotransferasas (AST/ALT. Todos los pacientes que concurrieron al laboratorio con solicitud de enzimograma hepático y anticuerpos IgM anti-VHA durante el período octubre 2005-marzo 2006 fueron evaluados en este estudio. Los datos clínicos más frecuentes fueron presunción de hepatitis e ictericia (27,5 y 12,7%. La determinación de IgM anti-VHA se realizó por ensayo inmunoenzimático de micropartículas (MEIA; la de enzimas hepáticas en un multianalizador. De los 158 pacientes estudiados, 84 tenían valores elevados de AST/ALT; dentro de este grupo, el 82% fue reactivo para IgM anti-VHA. Los 74 pacientes restantes mostraron niveles normales de AST/ALT y solo 7 de ellos, bajo control de evolución por VHA ya confirmada, fueron reactivos para IgM anti-VHA. El 49% de los pacientes IgM anti-VHA reactivos correspondió a menores de 10 años. De acuerdo con estas observaciones, sugerimos que los estudios de laboratorio para confirmar infección aguda por VHA deberían realizarse en forma secuencial, ya que es innecesaria la determinación de anticuerpos IgM anti-VHA cuando las aminotransferasas séricas son normales.Type A viral Hepatitis (HAV is the most frequent viral hepatitis around the world, especially in low income countries. In order to confirm this disease, a lot of laboratory tests are annually carried out where HAV is endemic. Our objective was to establish the utility of investigating anti-hepatitis A virus (HAV IgM antibodies for HAV diagnosis in patients with normal

Research of antibodies anti-brucella ovis in ovine the eight city of recôncavo baiano Detecção de anticorpos anti-"Brucella ovis" em ovinos do Estado da Bahia

Directory of Open Access Journals (Sweden)

Alessandra Estrela Silva Lima

2009-12-01

Full Text Available Ovine brucellosis is a contagious disease caused by Brucella ovis, characterized by clinical marked as epididymitis, abortion and lambs neonatal mortality, leading to reduction in reproductive efficiency of livestock and causing great economic damage. Considering the lack of seroepidemiologic data about B. Ovis in the State of Bahia and the importance of this disease in the economic sphere of sheep rearing, the design of this study aimed to achieve serum inquiry to investigate toe occurrence of anti-Brucella ovis in sheep in the Recôncavo Baiano. 183 serum samples were submited to the test of agar gel immunodiffusion (AGIO using soluble antigens produced by the Veterinary Research Center Oesidério Finamor (CPVOF in Rio Grande do Sul. Six (3.27% showed serological evidence of infection by B. Ovis Of 183 sera from sheep investigated by the AGIO to search for evidence of anti-Brucella ovis, with no significant difference between age and sex (p = 2.0 and p = 0.60, respectively of animais with the proportion of sheep seropositives. The results obtained by serological survey have been lead to accept that the infection by B. Ovis it is present in commercial herds of sheep in the state of Bahia, and requiring more extensive studies in sheep population, adoption of sanitary measures of prevention and control to prevent the spread of the disease.A brucelose ovina é uma doença de caráter contagioso, causada por Brucella ovis, caracterizada por um quadro clínico de epididimite, abortamento e mortalidade neonatal de cordeiros, levando a redução da eficiência reprodutiva dos rebanhos e provocando grandes perdas econômicas. Considerando a ausência de dados epidemiológicos, sobre a B. ovis no estado da Bahia e sua importância econômica para ovinocultura, o presente trabalho teve por objetivo realizar inquérito sorológico para investigar a ocorrência de anticorpos anti-Brucella ovis em ovinos no recôncavo baiano. Foram submetidas 183 amostras

Camellia sinensis L. Extract and Its Potential Beneficial Effects in Antioxidant, Anti-Inflammatory, Anti-Hepatotoxic, and Anti-Tyrosinase Activities

Directory of Open Access Journals (Sweden)

Surached Thitimuta

2017-03-01

Full Text Available The aims of this study were to investigate the potential benefits of antioxidant, anti-inflammatory, anti-hepatotoxic, and anti-tyrosinase activities of a methanolic extract of fresh tea leaves (FTE (Camellia sinensis L.. The antioxidant capacity was investigated using three different methods at different temperatures. The anti-inflammatory activity was studied in vitro by the inhibition of 5-lipoxygenase assay. The anti-hepatotoxic effect was investigated in CCl4-induced liver injury in rats. The anti-tyrosinase activities of the FTE and its principal phenolic compounds were investigated in l-3,4-dihydroxyphenylalanine (l-DOPA oxidation by a mushroom tyrosinase. A molecular docking study was conducted to determine how the FTE’s principal catechins interact with the tyrosinase. The FTE exhibited the best shelf life at low temperatures and demonstrated concentration-dependent antioxidant, anti-inflammatory, anti-hepatotoxic, and anti-tyrosinase effects compared to positive references. Treatment of rats with the FTE at 2000 mg/kg/day for 28 consecutive days reversed CCl4-induced oxidative damage in hepatic tissues by lowering the levels of alanine aminotransferase by 69% and malondialdehyde by 90%. Our findings suggest that the FTE has the capacity to scavenge free radicals and can protect against oxidative stress induced by CCl4 intoxication. The docking results were consistent with our in vitro data, indicating the anti-tyrosinase potency of the principal catechins.

Camellia sinensis L. Extract and Its Potential Beneficial Effects in Antioxidant, Anti-Inflammatory, Anti-Hepatotoxic, and Anti-Tyrosinase Activities.

Science.gov (United States)

Thitimuta, Surached; Pithayanukul, Pimolpan; Nithitanakool, Saruth; Bavovada, Rapepol; Leanpolchareanchai, Jiraporn; Saparpakorn, Patchreenart

2017-03-04

The aims of this study were to investigate the potential benefits of antioxidant, anti-inflammatory, anti-hepatotoxic, and anti-tyrosinase activities of a methanolic extract of fresh tea leaves (FTE) ( Camellia sinensis L.). The antioxidant capacity was investigated using three different methods at different temperatures. The anti-inflammatory activity was studied in vitro by the inhibition of 5-lipoxygenase assay. The anti-hepatotoxic effect was investigated in CCl₄-induced liver injury in rats. The anti-tyrosinase activities of the FTE and its principal phenolic compounds were investigated in l-3,4-dihydroxyphenylalanine (l-DOPA) oxidation by a mushroom tyrosinase. A molecular docking study was conducted to determine how the FTE's principal catechins interact with the tyrosinase. The FTE exhibited the best shelf life at low temperatures and demonstrated concentration-dependent antioxidant, anti-inflammatory, anti-hepatotoxic, and anti-tyrosinase effects compared to positive references. Treatment of rats with the FTE at 2000 mg/kg/day for 28 consecutive days reversed CCl₄-induced oxidative damage in hepatic tissues by lowering the levels of alanine aminotransferase by 69% and malondialdehyde by 90%. Our findings suggest that the FTE has the capacity to scavenge free radicals and can protect against oxidative stress induced by CCl₄ intoxication. The docking results were consistent with our in vitro data, indicating the anti-tyrosinase potency of the principal catechins.

Reactivo hemoclasificador monoclonal cubano hemo-cim anti-a: Estudio de estabilidad Cuban anti-A Hemo-CIM hemoclassifier monoclonal reagent: Stability study

Directory of Open Access Journals (Sweden)

Lilia Suárez Batista

1999-08-01

Full Text Available Los estudios de estabilidad de los reactivos fabricados a partir de anticuerpos monoclonales (AcM de origen murino, son esenciales para obtener resultados adecuados en su aplicación práctica y constituyen un requisito indispensable en las buenas prácticas de producción, lo que permite establecer adecuadamente la vida de estos productos. Se usaron los métodos de hemaglutinación recomendados para medir la actividad biológica del producto Hemo-CIM anti-A expresada en la potencia, la avidez y la intensidad frente a un panel de eritrocitos del grupo A1 y A2B, que se incluyó por su baja expresión del antígeno A para demostrar más efectivamente cualquier deterioro que sufriera el reactivo. Se estableció que la vida útil del reactivo hemoclasificador producido en el Centro de Inmunología Molecular es de 2 años y se determinó que la temperatura de almacenamiento del producto está entre 2 y 8 °C. Además, los lotes que se colocaron diariamente en la meseta de trabajo durante 5 horas a 21 °C a partir del mes 0 y a los 8, 14 y 22 meses después de su producción, simulando las condiciones a la que los usuarios someten a estos reactivos, mantuvieron las características de calidad que se requieren para su uso, lo que demostró que con este producto se puede trabajar en esas condicionesThe stability studies of the reactives obtained from monoclonal antibodies of murine origin are essential to attain adequate results in their practical application and are also an indispensable requirement for good production practices, which allow to establish the life of these products adequately. The hemagglutination methods were used to measure the biological activity of the anti-A Hemo-CIM product expressed in power, avidity and intesity against a set of erythrocytes of group A1 and A2B that was included due to its low antigen A expression to show more effectively any deterioration suffered by the reagent. It was determined that the useful life of the

Comportamento imunológico das vacinas anti-meningocócicas

Directory of Open Access Journals (Sweden)

Requejo Henry I. Z.

1997-01-01

Full Text Available A doença meningocócica continua sendo um grande problema de saúde pública em todos os continentes, e as vacinas anti-meningocócicas têm sido indicadas na prevenção e controle de epidemias. As vacinas polissacarídicas A e C são relativamente eficazes, com comportamentos imunológicos distintos frente às faixas etárias; no entanto, para o sorogrupo B, embora existam numerosos estudos internacionais até agora já desenvolvidos, ainda não se tem uma vacina altamente segura e eficaz de ampla aceitação. O polissacáride capsular do meningococo B não é imunogênico devido ao seu mimetismo com componentes celulares do hospedeiro. Tentativas de se introduzir carreadores protéicos vêm sendo feitas para se obter uma vacina que seja imunogênica em todas as faixas etárias e de preferência protetora contra todos os meningococos. Foi feita revisão da literatura com o objetivo de estudar o comportamento imunológico de todas as vacinas, até então desenvolvidas, e mostrar os esforços que estão sendo empreendidos no sentido de se buscar um produto seguro e eficaz para o controle da doença meningocócica

Comportamento imunológico das vacinas anti-meningocócicas

Directory of Open Access Journals (Sweden)

Henry I. Z. Requejo

1997-08-01

Full Text Available A doença meningocócica continua sendo um grande problema de saúde pública em todos os continentes, e as vacinas anti-meningocócicas têm sido indicadas na prevenção e controle de epidemias. As vacinas polissacarídicas A e C são relativamente eficazes, com comportamentos imunológicos distintos frente às faixas etárias; no entanto, para o sorogrupo B, embora existam numerosos estudos internacionais até agora já desenvolvidos, ainda não se tem uma vacina altamente segura e eficaz de ampla aceitação. O polissacáride capsular do meningococo B não é imunogênico devido ao seu mimetismo com componentes celulares do hospedeiro. Tentativas de se introduzir carreadores protéicos vêm sendo feitas para se obter uma vacina que seja imunogênica em todas as faixas etárias e de preferência protetora contra todos os meningococos. Foi feita revisão da literatura com o objetivo de estudar o comportamento imunológico de todas as vacinas, até então desenvolvidas, e mostrar os esforços que estão sendo empreendidos no sentido de se buscar um produto seguro e eficaz para o controle da doença meningocócica

Anti-Bubbles

Science.gov (United States)

Tufaile, Alberto; Sartorelli, José Carlos

2003-08-01

An anti-bubble is a striking kind of bubble in liquid that seemingly does not comply the buoyancy, and after few minutes it disappears suddenly inside the liquid. Different from a simple air bubble that rises directly to the liquid surface, an anti-bubble wanders around in the fluid due to its slightly lesser density than the surrounding liquid. In spite of this odd behavior, an anti-bubble can be understood as the opposite of a conventional soap bubble in air, which is a shell of liquid surrounding air, and an anti-bubble is a shell of air surrounding a drop of the liquid inside the liquid. Two-phase flow has been a subject of interest due to its relevance to process equipment for contacting gases and liquids applied in industry. A chain of bubbles rising in a liquid formed from a nozzle is a two-phase flow, and there are certain conditions in which spherical air shells, called anti-bubbles, are produced. The purpose of this work is mainly to note the existence of anti-bubbling regime as a sequel of a bubbling system. We initially have presented the experimental apparatus. After this we have described the evolution of the bubbling regimes, and emulated the effect of bubbling coalescence with simple maps. Then is shown the inverted dripping as a consequence of the bubble coalescence, and finally the conditions for anti-bubble formation.

Efeito da adição de aminoácidos essenciais à dieta sobre a secreção de enzimas digestivas de jundiá Rhamdia quelen (Siluriformes, Pimelodidae = Effect of dietary essential amino acids addition on digestive enzime secretion in silver catfish Rhamdia quelen (Siluriformes, Pimelodidae

Directory of Open Access Journals (Sweden)

Alexandre Bernardini Ungar

2009-01-01

Full Text Available Em estudos realizados com variação no teor de proteína na ração para peixes, foi demonstrado o aumento das atividades de protease alcalina, protease ácida, tripsina e quimiotripsina do trato digestório de jundiá (Rhamdia quelen. Esses fatos sugerem que produtos liberados pela digestão na luz desse trato podem influenciar a síntese e a secreção de enzimas digestivas. A secreção destas enzimas pelo pâncreas, em vertebrados, responde à influência neurale humoral. No presente trabalho, foram testadas quatro concentrações (0, 3, 6 e 12% de uma mistura de aminoácidos (treonina, fenilalanina, leucina, valina, arginina e triptofano em iguaisproporções em dietas isocalóricas contendo 3.500 kcal de energia digestível kg-1 de ração, e dietas isoproteicas contendo 20% de proteína, em juvenis de jundiá. Foram utilizados dez animais portratamento, sendo os peixes estocados num sistema fechado com recirculação de água e temperatura controlada. Os peixes submetidos a estes tratamentos apresentaram atividade da protease alcalina na região anterior do intestino, responsiva aos diferentes níveis aminoácidos da alimentação. Todavia, as atividades protease alcalina do fígado e amilase do fígado e intestino anterior não foram detectadas. Estes resultados sugerem que a atividade protease alcalina do intestino anterior seja induzida por aminoácidos liberados na luz do trato digestório. Studies carried out with variation of protein concentration in the fish feed have shown an increase in the activity of alkaline proteases, acid proteases, trypsin and chimotrypsin from the digestive tract of silver catfish (Rhamdia quelen. These facts suggest that products released by digestion in the lumen of this tract can influenced the synthesis and secretion of digestive enzymes. The secretion of these enzymes by the pancreas in vertebrate responses to neural and humoral influences. In the present study, four concentration (0, 3, 6 and 12% of

Frequence of antibodies for Neospora caninum and Toxoplasma gondii in dogs in northest of São Paulo State/ Freqüência de anticorpos anti- Neospora caninum e anti- Toxoplasma gondii em cães da região nordeste do Estado de São Paulo. Correlação com neuropatias

Directory of Open Access Journals (Sweden)

Alvimar José da Costa

2001-08-01

Full Text Available Two hundred and ninety five samples of canine sera, of dogs from northeast of Sao Paulo State were tested using an immunofluorescent antibody technique (RIFI-IgG for the presence of Neospora caninum and Toxoplasma gondii antibodies. The relationship between antibodies and casual clinical problems also were investigated. The reactions with title = 50 (N. caninum and = 16 (T. gondii were considered positive. A total of 25 (8,48% of tested samples werereactive and 270 (91,52% non-reactive to N. caninum, while 151 (51,19% of the samples were reactive and 144 (48,81% were non-reactive to T. gondii. Dogs with serum that reacted positively to both protozons parasites were seen in 17 animals (5,76%. From 58 dogs (19,66%, that showed nervous sintomatology, were detected antibodies in 04 (1,36% to N. caninum and in 24 (8,14% to T. gondii. Statistical differences (PInvestigou-se a presença de anticorpos anti- N. caninum e anti- T. gondii em 295 soros colhidos de cães oriundos da região Nordeste do Estado de São Paulo, com o objetivo de correlacionar a presença de anticorpos com eventuais distúrbios clínicos. Todas as amostras de soros foram examinadas pela reação de imunofluorescência indireta (RIFI para ambos protozoários. As reações com recíprocas de títulos igual ou maior que 50 (N. caninum e 16 ( T. gondii foram consideradas positivas. Diluições seqüenciais foram efetuadas até a obtenção do título sorológico final. Os resultados revelaram que 25 (8,48% e 151 (51,19% dos cães examinados apresentavam anticorpos anti- N. caninum e anti- T. gondii, respectivamente. Anticorpos para ambos os coccídios foram detectados em 17 animais (5,76%. Dos 58 cães (19,66% que apresentavam sintomatologia nervosa, foram detectados anticorpos em 04 (1,36% para N. caninum e em 24 (8,14% para T. gondii. Apesar da literatura atribuir a ambos coccídios a etiologia de distúrbios neurológicos em cães, os resultados obtidos não revelaram correla

(Reclaiming an activist identity as criticalmathematics educators: addressing anti-black racism because #BlackLivesMatter

Directory of Open Access Journals (Sweden)

Paula Groves Price

2016-01-01

Full Text Available Durante el 8vo Encuentro Internac ional Mat emá ticas Educa ción y Socie dad (MES - 8, Martin (2015 plante ó una cuestión fundamental , “ ¿ En dónde se posiciona la educació n matemática crítica y demuestra acció n inequívoca contra el racismo anti - negro , el terror racial y la supremacía blanca global? Esta pregunta marca un llamado a la acción para los educadores matemáticos críticos a pensar no solamente en las posibilidades liberadoras y emancipadoras de la educación matemática , sino de abordar de manera más explícita y específicamente la raza y el racismo . Usando la “definición futurista” de Powell (2012 de educa dor m ate m á tico crí tico como matemático, educador y activista, las autoras plantean que aunque los educador es matemático s crítico s han conducido exitosamente a que los educadores consideren mejores estrategias pedagógicas para alcanzar una mayor equidad , es necesaria más acción social para luchar contra el racismo. Las autoras abogan por una mayor atención al llamado a la acción que hizo Martin y a la necesidad de que los educadores matemáticos críticos se involucr en activa y ex plícitamente en má s acciones inequívocas en contra de la supremacía blanca global y el racismo anti - negro.

Anti-idiotypic antibodies directed against anti-HBs among the patients with chronic hepatitis B.

Science.gov (United States)

Kobayashi, K; Suzuki, H; Ueno, Y; Nagatomi, R; Kanno, A; Otsuki, M; Toyota, T

1990-08-01

Anti-idiotypic antibodies (anti-Id) against anti-HBs were found in the sera of patients with chronic hepatitis type B. Anti-idiotypic antibodies were detected by an enzyme-linked immunosorbent assay using horseradish peroxidase conjugated mouse monoclonal anti-HBs. Ten of 72 HBsAg positive sera contained anti-Id (13.9%). The prevalence of anti-Id did not appear to correlate with HBeAg/anti-HBe system. However, HB virus specific DNA polymerase activity was significantly higher in anti-Id positive sera. In the sera obtained from the patients treated with predonisolone before, anti-Id positive rate was higher than that in the patients without a history of predonisolone therapy. These results suggest that anti-Id may be related to the immunoregulatory mechanism of HB virus replication.

Auto-anticorpos anti-β2-glicoproteína I e síndrome metabólica

Directory of Open Access Journals (Sweden)

Rodrigo B. Krás Borges

2011-04-01

Full Text Available FUNDAMENTO: A síndrome metabólica (SM é uma entidade pró-aterogênica. Autoanticorpos tais como β2-glicoproteína I (β2-gpI podem influenciar o aparecimento de ateromas. Estudos anteriores confirmaram uma associação entre anticorpos IgA anti-β2-gpI e isquemia cerebral, infarto do miocárdio, doença arterial periférica e doença da carótida. OBJETIVO: O objetivo desse estudo de caso-controle foi avaliar uma possível associação entre anticorpos anti-β2-gpI e anticardiolipina (aCL com SM não-complicada. MÉTODOS: Pacientes com SM sem histórico de eventos vasculares e indivíduos-controle, consistindo em pacientes da Enfermaria de Ortopedia admitidos devido a doenças musculoesqueléticas foram incluídos no estudo. Idade, sexo, etnia, histórico de hipertensão, tabagismo, hipercolesterolemia e diabetes mellitus foram avaliados como fatores de risco em ambos os grupos. Anticorpos IgG, IgM, e IgA anti-β2-gpI e aCL foram detectados através de imunoensaios enzimáticos. RESULTADOS: Um total de 68 pacientes com SM e 82 controles foram estudados. Os pacientes com SM tinham média de idade superior à dos controles (P = 0,001, enquanto homens (P = 0,003; OR 0,31; IC95%: 0,15-0,16 e etnia caucasiana (P = 0,004; OR 0,25; IC95%:0,10-0,60 eram predominantes nos controles. Histórico de hipertensão, hipercolesterolemia e diabetes mellitus foi mais prevalente nos pacientes com SM do que nos controles (P < 0.05. A frequência de anticorpos aCL (todos os isotipos e do IgG e IgM anti-β2 gpI não diferiu de forma significante nos pacientes com SM e controles. Anticorpos IgA anti-β2-gpI foram significantemente mais frequentes nos pacientes com SM (42,2% do que nos controles (10,9% (P < 0,001. O OR ajustado para anticorpos IgA anti-β2-gpI foi 3,60 (IC95%: 1,55-8,37; P = 0,003. CONCLUSÃO: O presente estudo mostra que níveis elevados de autoanticorpos IgA para β2-gpI podem estar independentemente associados com SM.

Adesão de linhagem selvagem de Streptococcus thermophilus em superfície de aço inoxidável e efeitos da higienização na sua remoção Adhesion of a wild strain of Streptococcus thermophilus onto stainless steel surfaces and the effects of cleaning and sanification on its removal

Directory of Open Access Journals (Sweden)

Ana Lourdes Neves GÂNDARA

2000-04-01

Full Text Available Linhagem selvagem de Streptococcus thermophilus isolada de leite pasteurizado foi avaliada em modelo experimental quanto a adesão em superfície de aço inoxidável e comportamento frente à limpeza e sanificação. Em leite, a adesão do microrganismo em aço inoxidável foi estudada em 6h de contato a 45°C sob agitação e uma higienização com detergentes alcalino e ácido seguida de sanificação foi utilizada para avaliação do comportamento das células aderidas frente à higienização. Esse microrganismo aderiu a essa superfície produzindo uma carga de 10(4UFC/cm². Após a limpeza alcalina não foram detectadas células aderidas; em seguida a limpeza ácida 6 UFC/cm² ainda foram detectadas. A sanificação com hipoclorito de sódio, após a limpeza, foi suficiente para reduzir a carga de S. thermophilus selvagem aderida ao aço inoxidável. O modelo experimental mostrou-se adequado para o estudo, indicando que a cultura selvagem de Streptococcus thermophilus é produtora de biofilme em superfície de aço inoxidável. A limpeza da superfície de aço inoxidável por detergência alcalina remove mais que 99,9% das células aderidas. Pequenos números de células remanescentes são removidos na detergência ácida o que demonstra a necessidade das diferentes etapas e tipos de detergentes para a eficiência da limpeza. Melhores resultados na remoção desse biofilme são alcançadas com detergência alcalina seguida de detergência ácida e mais eficientemente quando se utiliza uma sanificação complementar com hipoclorito de sódio.A wild strain of Streptococcus thermophilus isolated from pasteurized milk was evaluated using an experimental model with respect to its adhesion onto stainless steel surfaces and its behaviour when submitted to cleansing and sanification. In milk, the adhesion of the microorganism on to stainless steel surfaces was studied after 6 hours of contact at 45°C with agitation, and after a cleansing process

Presença de anticorpo anti-Lutheran B em paciente com leucemia linfóide aguda Anti-Lutheran B in a patient with acute lymphoblastic leukemia

Directory of Open Access Journals (Sweden)

Maria do Rosário F. Roberti

2007-06-01

Full Text Available Leucemias agudas necessitam de suporte hemoterápico para serem tratadas adequadamente. A presença do anticorpo anti-Lutheran B e a alta freqüência do antígeno em nossa população dificulta o tratamento de doenças hematológicas que necessitam de suporte transfusional, tais como a leucemia linfóide aguda (LLA. Alternativas de tratamento hemoterápico são abordadas neste artigo. Descreve-se o caso de uma paciente com LLA e anticorpo anti-Lutheran B. A dificuldade em encontrar sangue compatível levou os autores a adotarem estratégia conservadora no tratamento hemoterápico. O uso de eritropoetina e a adoção de protocolo quimioterápico individualizado viabilizaram os resultados obtidos.Acute leukemia needs hemotherapeutic support to be successfully treated. The Lutheran B antibody, specifically the high frequency of this antigen in our population, has made it difficult to treat hematological malignancies that need transfusional support, including acute lymphoblastic leukemia. Finding compatible blood is complex and so the authors adopted a conservative treatment strategy. The prescription of epoetin and an individualized chemotherapy protocol may improve results. We describe a case report of a patient with anti-Lutheran B antibody and how difficulties to find compatible blood were solved.

Produção de anticorpos policlonais anti-ricina Production of polyclonal anti-ricin antibodies

Directory of Open Access Journals (Sweden)

Roselayne Ferro Furtado

2011-02-01

Full Text Available A ricina é uma proteína bastante tóxica presente nas sementes de mamona que impossibilita o uso da torta de mamona "in natura", como ração. A torta de mamona destoxificada necessita ainda de métodos de análise que garantam a ausência de traços dessa proteína. Objetivou-se, neste trabalho, produzir e avaliar a sensibilidade e especificidade de anticorpos policlonais anti-ricina, para serem empregados como possíveis componentes de métodos sorológicos na detecção de ricina em torta de mamona destoxificada. Foram avaliadas três doses da proteína: 400, 180 e 100 µg cada uma dividida em duas aplicações em coelhos. A primeira dose foi injetada no animal no início do experimento e a segunda após 21 dias. O método de ELISA indicou que as duas doses menores (100 e 180 µg induziram respostas imunológicas primária e secundária com produção de anticorpos específicos. Enquanto a dose maior (400 µg de ricina apresentou uma resposta primária com elevação dos títulos de anticorpos, seguida de uma supressão da resposta. Esse perfil é sugestivo de tolerância imunológica. Pela técnica de Western blotting verificou-se que os anticorpos policlonais produzidos são bastante específicos para a ricina, no entanto, por detectarem ricina na forma nativa e desnaturada não são recomendados para o monitoramento de ricina em torta de mamona destoxificada por tratamento térmico.Ricin is a very toxic protein found in castor bean plants, making it impossible to use natural castor cake as animal food. The detoxificated castor cake needs to be analyzed by methods that ensure the absence of traces of this protein. This work had the objective to produce and to evaluate the sensitivity and specificity of anti-ricin polyclonal antibodies, to be employed as component of sorologic methods as the ELISA in the detection of ricin in detoxificated castor cake. Three doses of protein, 400, 180 and 100 µg were evaluated each one injected twice into

Intravenous Immunoglobulin therapy for anti-E hemolytic disease in the newborn.

Science.gov (United States)

Onesimo, Roberta; Rizzo, Daniela; Ruggiero, Antonio; Valentini, Piero

2010-09-01

Anti-E alloimmunisation is a less common cause of haemolytic disease in the newborn (HDN) and is usually associated with mild to moderate clinical manifestations, that are often less severe than anti-D immunisation. Conventional treatments for HDN are phototherapy and exchange transfusion, the latter still representing a high-risk procedure. Currently, intravenous immunoglobulin has been used as alternative treatment for HDN to reduce the need for exchange transfusion, as well as the length of phototherapy and hospitalisation. We report a case of anti-E HDN treated successfully with intravenous immunoglobulin, as adjuvant treatment to phototherapy.

PRODUKSI ANTIBODI KUNING TELUR (IGY ANTI STREPTOCOCCUS MUTANS SEBAGAI ANTI KARIES GIGI

Directory of Open Access Journals (Sweden)

Okti Nadia Poetri

2006-12-01

Full Text Available The aim of this study was to explore IgY anti Streptococcus mutan production and the ability of Igy Streptococcus mutans blocking adhesion process. The eggs was collected from Single Comb Brown Leghorn which have been immunized by S. mutan. Agar gel precipitation test was done to detect IgY anti S. mutans in serum and egg. Egg which Countain IgY anti S. mutans was collected. IgY anti S. mutans extracted from egg yolk by mean s PEG-Amonium sulfat and purified using fast protein liquid chromatography. The purity of Igy anti S. mutans was determined by UV spectropometer. Biological activities of Igy anti S. mutans to inhibit adhession process was learned by anti adhesion test. We use two dose of IgY, which is 100 ug and 500 ug. Igy anti S. mutans formen in serum five weeks after the first immunization while it formed in egg nine weeks after the first immunization. Igy anti S. mutanss still present in serum andegg until twelve weeks from the first immunization. Igy anti S. mutanss could decrease the amount of bacteria which attach the epithelial cell surface. The amount of sticky bacteria on epithelial cell (without IgY are 40 cell bacteria/epithelial cell. After blocked by IgY anti S. mutanss the amount of bacteria turn into 30 cell bacteria/epithelial cell (for dose of 100 ug IgY and 28 cell bacteria/epitheelial cell (for dose of 500 ug IgY. This research concluded that hens were capable producing IgY anti S. mutanss in egg yolk and it can be used to solve dental caries problem which caused by S. mutanss.

Explicit and implicit effects of anti-marijuana and anti-tobacco TV advertisements.

Science.gov (United States)

Czyzewska, Maria; Ginsburg, Harvey J

2007-01-01

Effects of anti-tobacco and anti-marijuana TV advertisements on explicit (i.e., semantic differential ratings) and implicit (i.e. Implicit Association Test, IAT) attitudes toward tobacco and marijuana were compared. Two hundred twenty nine, 18- to 19-year-old U.S. college students were randomly assigned to anti-tobacco or anti-marijuana PSA viewing conditions. Participants completed a short survey on attitudes to tobacco and marijuana. Afterwards they watched 15 PSAs embedded in a 15-min science program. At the end, all participants completed IAT for marijuana, IAT for tobacco and the assessment of explicit attitudes. Results of ANCOVA revealed a significant interaction between type of TV PSAs watched and implicit attitudes, F(1,223)=7.12, padvertisements watched (i.e., anti-tobacco or anti-marijuana). However, analogical analysis on explicit measures showed that attitudes to marijuana became less negative among students that watched anti-marijuana ads than the group with anti-tobacco ads, F(1,222)=5.79, p<0.02. The discussion focused on the practical and theoretical implications of the observed dissociation between implicit and explicit attitudes to marijuana after the exposure to anti-marijuana PSAs.

The prozone effect exerted by the complement-binding anti-Lea on anti-D.

Science.gov (United States)

Joshi, Sanmukh R; Parekh, Kamlesh H

2017-01-01

Prozone phenomenon is seen with very high-titer antibodies in an immune serum. The prozone effect on anti-D by a low-titer anti-Le a was investigated associated with neonatal jaundice. Standard methods were used in investigations. The child was born at full-term developed mild jaundice. With weak direct antiglobulin test+, her indirect serum bilirubin was progressed to 27.5 mg/dL in 48 h. Anti-D and anti-Le a were detected in the mother. Both these antibodies were detected in the child's serum though the eluate from red blood cells (RBCs) contained only anti-D. Mother's anti-D was masked by anti-Le a if the RBCs possessed both the antigens together. Anti-D was revealed only with D-positive RBCs lacking Le a or if the serum was modified by mixing with Le a+ saliva or was heated at 56°C or fortified with citrate solution. An anti-D showed prozone effect exerted by the complement-fixing anti-Le a in the test.

The anti pp yields anti λ λ reaction near threshold

International Nuclear Information System (INIS)

Maher, C.J.

1986-06-01

Measurements of differential and integrated cross sections as well as final state polarizations for the anti p p → anti Λ Λ reaction are presented. The reaction was studied at two incident antiproton momenta (1476.5 MeV/c and 1507.5 MeV/c) corresponding to total center of mass energies of 15.5 MeV and 26.4 MeV above the reaction threshold. The trajectories of charged decay products of the anti Λ and Λ were observed in a multiwire proportional chamber and in two sets of drift chambers. The data were analyzed with a computer program which reconstructed anti p p → anti Λ Λ → anti p π + pπ - events and performed kinematic fitting. The results are compared to several recent meson exchange calculations, and a one-gluon exchange calculation. The experiment was performed at the Low Energy Antiproton Ring (LEAR) at CERN. The data presented represent the first results of the PS185 collaboration's study of the threshold production of hyperon-antihyperon states

Adesão ao tratamento farmacológico anti-hipertensivo de pacientes em hemodiálise

Directory of Open Access Journals (Sweden)

Paulo Roberto da Silva Ribeiro

2015-10-01

Full Text Available O risco de morbidade/mortalidade por doenças cardiovasculares em pacientes com doença renal crônica (DRC é elevado, sendo imprescindível a instituição de uma terapia anti-hipertensiva eficaz. A baixa adesão ou não adesão ao tratamento farmacológico anti-hipertensivo prejudica o alcance dos resultados terapêuticos no retardo da progressão da DRC. Objetivou-se estudar a adesão à terapia anti-hipertensiva em 120 pacientes com DRC em hemodiálise. Trata-se de um estudo transversal, descritivo, com abordagem quantitativa, realizado entre maio a junho de 2011 na Clínica de Doenças Renais de Imperatriz – MA. Os dados foram obtidos através da análise dos prontuários dos pacientes e da aplicação de roteiro de entrevista para a coleta de dados sociodemográficos, clínicos e relacionados ao tratamento medicamentoso anti-hipertensivo. O nível de adesão ao tratamento foi estimado pelo Teste de Morisky- Green. A maioria dos pacientes é do sexo masculino (69,2%, possui idade inferior a 60 anos (69,2%, é analfabeta ou possui apenas o ensino fundamental (79,2% e possui baixa renda mensal (90,8%. A hipertensão arterial foi a doença de base prevalente. Houve predomínio da politerapia em 78,3% dos casos. Os betabloqueadores foram os anti-hipertensivos mais utilizados, seguidos pelos bloqueadores dos canais de cálcio e agentes que atuam no sistema renina-angiotensina. O Teste de Morisky-Green apontou que 58,3% dos entrevistados apresentaram nível elevado de adesão à terapia medicamentosa. A falta de acesso aos medicamentos foi o principal motivo relatado pelos pacientes para não seguirem adequadamente o tratamento anti-hipertensivo. Verificou-se a necessidade de melhorias na assistência farmacêutica a estes pacientes e a implementação de estratégias para aumentar a adesão ao tratamento.Palavras-chave: Adesão à medicação. Anti-hipertensivos. Insuficiência Renal Crônica. Diálise Renal. Farmac

Production of d, t, 3He, anti d, anti t and anti 3He by 200 GeV protons

International Nuclear Information System (INIS)

Bozzoli, W.; Giacomelli, G.; Rimondi, F.; Zylberajch, S.; Lesquoy, E.; Meunier, R.; Moscoso, L.; Muller, A.; Bussiere, A.

1978-01-01

Data are presented on the yields of d, t, 3 He, anti d, anti t, and anti 3 He with laboratory momenta between 12 and 37 GeV/c produced by 200 GeV protons on beryllium and aluminium. The production yield of nuclei depends significantly on the target nucleus, while the anti d production is independent of target material. The mass dependence of the production cross section is exponential for both nuclei and antinuclei

Serum total and bone alkaline phosphatase and tartrate-resistant acid phosphatase activities for the assessment of bone fracture healing in dogs

Directory of Open Access Journals (Sweden)

C. Sousa

2011-08-01

Full Text Available O objetivo deste trabalho foi estudar o padrão de variação da atividade sérica da fosfatase alcalina total (tALP, da isoenzima óssea da fosfatase alcalina (BALP e da fosfatase ácida resistente ao tartarato (TRAP, assim como a variação da concentração dos minerais séricos durante o processo de cicatrização de fraturas ósseas no cão. A variação sérica destes marcadores do metabolismo ósseo foi avaliada em nove cães com fraturas diafisárias fechadas de ossos longos, submetidas a tratamento cirúrgico para osteosíntese. Durante o período pós-operatório, sete animais evoluíram no sentido de uma normal união óssea, sendo que dois deles desenvolveram um processo de não união óssea. Foram observados, relativamente à BALP, valores de actividade sérica mais elevados e com diferença estatística (P<0,05 no grupo de animais que evoluiu no sentido de uma normal união óssea, comparativamente ao grupo de animais que evoluiu no sentido do processo de não união. No grupo de animais que evoluiu para a completa união óssea foram, adicionalmente, observados valores diminuidos (P<0,05 da atividade sérica da TRAP, até ao dia 60 do período pós-operatório seguido de uma elevação estatisticamente significativa após este período. Em conclusão, os biomarcadores do metabolismo ósseo poderão vir a constituir um método auxiliar de diagnóstico na monitorização do processo de cicatrização de fracturas ósseas, possibilitando, a detecção precoce de complicações pós-operatórias.

A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos

Directory of Open Access Journals (Sweden)

Fernando L. GONÇALES JR

2000-06-01

RIBA-2 positive subjects, in 37.5% of the indeterminate RIBA-2 donors and in 9% of the negative RIBA-2 donors. Chronic hepatitis has also been observed in 50% of the histopathological exams of the anti-HCV EIA-2 reagent donors which were indeterminate RIBA-2. Among 18 blood donors with minimal changes histopathological exam 11 (61% were HCV-RNA positive. Our blood donors anti-HCV reagent generally had clinical, laboratorial and histopathological features observed in patients with chronic HCV hepatitis and a high proportion could be identified in interviews and medical evaluation realized in blood blanks. Generally, these HCV infected donors are identified and discharged only by the serological tests results.Entre 1992 e 1997 foram avaliados, ambulatorialmente, 790 doadores de sangue com teste anti-HCV EIA-2 fortemente reagente (relação entre a densidade ótica da amostra / "cut-off" > 3, que haviam sido detectados na triagem sorológica do banco de sangue. Todos eram negativos para doença de Chagas, sífilis, hepatite B (HBsAg e AIDS. Amostras de sangue foram coletadas, na primeira consulta ambulatorial, para a realização de hemograma, exames bioquímicos e novos testes sorológicos para a HVC (anti-HCV EIA-2. Em 226 doadores anti-HCV EIA-2 repetidamente reagentes, realizou-se o teste suplementar de "immunoblot" para a HVC (RIBA-2. Em 209 doadores, pesquisou-se a presença do RNA do VHC pelo teste do PCR, através de exame automatizado (HCV-AMPLICOR, ROCHE. A ultra-sonografia abdominal foi realizada em 366 doadores e a biópsia hepática em 269 concordantes. Notou-se que 95,6% eram EIA-2 repetidamente reagentes, 94% eram assintomáticos e que apenas 2% referiram icterícia pregressa. Em 47% detectou-se, pelo menos, um fator de risco para a transmissão do VHC, sendo o uso de drogas E.V. o principal deles (27,8%. A transfusão de sangue foi o segundo fator na transmissão da HVC (27,2%. Hepatomegalia foi encontrada em 54%. Esplenomegalia e sinais de hipertens

Herba Cistanches: Anti-aging

OpenAIRE

Wang, Ningqun; Ji, Shaozhen; Zhang, Hao; Mei, Shanshan; Qiao, Lumin; Jin, Xianglan

2017-01-01

The Cistanche species (“Rou Cong Rong” in Chinese) is an endangered wild species growing in arid or semi-arid areas. The dried fleshy stem of Cistanches has been used as a tonic in China for many years. Modern pharmacological studies have since demonstrated that Herba Cistanches possesses broad medicinal functions, especially for use in anti-senescence, anti-oxidation, neuroprotection, anti-inflammation, hepatoprotection, immunomodulation, anti-neoplastic, anti-osteoporosis and the promotion ...

Herba Cistanches: Anti-aging.

Science.gov (United States)

Wang, Ningqun; Ji, Shaozhen; Zhang, Hao; Mei, Shanshan; Qiao, Lumin; Jin, Xianglan

2017-12-01

The Cistanche species ("Rou Cong Rong" in Chinese) is an endangered wild species growing in arid or semi-arid areas. The dried fleshy stem of Cistanches has been used as a tonic in China for many years. Modern pharmacological studies have since demonstrated that Herba Cistanches possesses broad medicinal functions, especially for use in anti-senescence, anti-oxidation, neuroprotection, anti-inflammation, hepatoprotection, immunomodulation, anti-neoplastic, anti-osteoporosis and the promotion of bone formation. This review summarizes the up-to-date and comprehensive information on Herba Cistanches covering the aspects of the botany, traditional uses, phytochemistry and pharmacology, to lay ground for fully elucidating the potential mechanisms of Herba Cistanches' anti-aging effect and promote its clinical application as an anti-aging herbal medicine.

Anti-arrythemic and anti-hyperlipidaemic potentials of aqueous ...

African Journals Online (AJOL)

Anti-arrythemic and anti-hyperlipidaemic potentials of aqueous garlic extract in hyper-cholesterolaemic rat. H U Nwanjo, G O Oze. Abstract. No Abstract. IJONAS Vol. 3 (2) 2007: pp. 214-221. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT.

Repercussões temporais da ligadura do ducto biliar principal em ratos Wistar

Directory of Open Access Journals (Sweden)

Maia Eugenio Lincoln Campos

2003-01-01

Full Text Available OBJETIVO: Objetivou-se investigar os efeitos temporais da ligadura do ducto biliar principal, em ratos Wistar. MÉTODOS: Utilizaram-se 48 ratos machos distribuídos em 2 grupos e redistribuídos em 4 subgrupos (n=06, identificados pelo tempo estipulado para a coleta das amostras (12, 24, 48 e 168 horas. Após laparotomia, os animais foram submetidos à dissecção e passagem de dois fios circundando o ducto biliar principal, sem ligadura (Grupo 1 e com ligadura (Grupo 2. RESULTADOS: As concentrações das bilirrubinas séricas (total, direte e indireta quando comparadas ao repectivo grupo controle, aumentaram (p<0,05 nos animais do grupo 2, nos tempos 24, 48 e 168 horas. As concentrações de proteína C reativa e fosfatase alcalina aumentaram (p<0,05 no grupo 2 no tempo 24 horas e nos tempos 24 e 48 horas, respectivamente. Concentrações de albumina e gamaglutamil transferase, não apresentaram diferenças significantes nos dois grupos. Foram observadas, no exame histopatológico, hiperplasia ductal, pericolangite e colangite no grupo 2 e pericolangite no grupo 1. CONCLUSÕES: A ligadura do ducto biliar principal induz aumento das concentrações séricas de bilirrubinas, proteína C reativa e fosfatase alcalina. Hiperplasia ductal, pericolangite e colangite são as principais alterações histológicas encontradas nos fígados dos ratos submetidos à ligadura do ducto biliar principal.

Approach to a Pregnant Woman with Anti D + Anti C Reactivity Pattern: A Diagnostic Conundrum.

Science.gov (United States)

Rai, Preeti; Sharma, Geetika; Singh, Deeksha; Garg, Jyoti

2017-09-01

The Rhesus G antigen is present on all RBCs that are C+ and also on most D+ RBCs. Due to this co-distribution of G with either C or D antigen, it mimics a reactivity pattern of anti C + anti D on Indirect Antiglobulin Test (IAT), though the role of Anti G in causing Hemolytic Disease of Newborn (HDN) is controversial. The differentiation of anti D, anti C, and anti G is essential particularly in pregnant females. We hereby report a rare case of anti G with anti D and anti C in a pregnant woman with emphasis on approach to identify anti D+C+G and its implications.

Hemolytic disease of the fetus and newborn caused by anti-E

OpenAIRE

Usman, Adiyyatu Sa?idu; Mustaffa, Rapiaah; Ramli, Noraida; Diggi, Sirajo A.

2013-01-01

Objective: Maternal allo-antibody production is stimulated when fetal red blood cells are positive for an antigen absent on the mother′s red cells. The maternal IgG antibodies produced will pass through the placenta and attack fetal red cells carrying the corresponding antigen. Allo-immune hemolytic disease of the fetus and newborn caused by anti-E rarely occurs. Case summary: We report two cases of anti-E hemolytic diseases in neonates. One of the neonates had severe hemolysis presenting wit...

Anti-inflammatory, anti-cholinergic and cytotoxic effects of Sida rhombifolia.

Science.gov (United States)

Mah, Siau Hui; Teh, Soek Sin; Ee, Gwendoline Cheng Lian

2017-12-01

Sida (Malvaceae) has been used as a traditional remedy for the treatment of diarrhoea, malarial, gastrointestinal dysentery, fevers, asthma and inflammation. This study evaluates the anti-inflammatory, cytotoxic and anti-cholinergic activities of Sida rhombifolia Linn. whole plant for the first time. S. rhombifolia whole plant was extracted by n-hexane, ethyl acetate and methanol using Soxhlet apparatus. The plant extracts were evaluated for their antioxidant (DPPH, FIC and FRAP), anti-inflammatory (NO and protein denaturation inhibitions), cytotoxic (MTT) and anti-cholinesterase (AChE) properties in a range of concentrations to obtain IC 50 values. GC-MS analysis was carried out on the n-hexane extract. The ethyl acetate extract exhibited the most significant antioxidant activities by scavenging DPPH radicals and ferrous ions with EC 50 of 380.5 and 263.4 μg/mL, respectively. In contrast, the n-hexane extract showed the strongest anti-inflammatory activity with IC 50 of 52.16 and 146.03 μg/mL for NO and protein denaturation inhibition assays, respectively. The same extract also revealed the strongest effects in anti-cholinesterase and cytotoxic tests at the concentration of 100 μg/mL, AChE enzyme inhibition was 58.55% and human cancer cells, SNU-1 and Hep G2 inhibition was 68.52% and 47.82%, respectively. The phytochemicals present in the n-hexane extract are palmitic acid, linoleic acid and γ-sitosterol. The present study revealed that the n-hexane extract possessed relatively high pharmacological activities in anti-inflammation, cytotoxicity and anti-cholinesterase assays. Thus, further work on the detail mechanism of the bioactive phytochemicals which contribute to the biological properties are strongly recommended.

Preparation of the radiopharmaceutical {sup 131}I-Anti-CD20 for the treatment of lymphomas; Preparacion del radiofarmaco {sup 131}I-Anti-CD20 para el tratamiento de linfomas

Energy Technology Data Exchange (ETDEWEB)

Pantoja H, I E

2004-07-01

At the present time they are considered to the lymphomas like a problem of first magnitude since has happened it is necessary to be the fifth cancer cause in the world. Different treatments focused to the lymphoma like the chemotherapy and the radiotherapy, have been employees to counteract the No-Hodgkin lymphoma, without these they don't exclude the healthy tissue of the toxicity. It is for it that is taking a new direction with the employment of the directed radioimmunotherapy since this it allows to kill wicked cells selectively with radiation dose joined to the apoptosis and cytotoxicity induced by the own one bio molecule. The radioimmunotherapy with radiolabelled antibodies directed to the surface antigen CD20 represents a new modality for the treatment of No-Hodgkin lymphoma and potentially other illnesses. In this work the parameters of optimization are presented for the preparation, control of quality and evaluation of the stability in vitro and in vivo of the monoclonal antibody anti-CD20 labelled with {sup 131} I for the treatment of No-Hodgkin lymphoma. The anti-CD20 labelled by the chloramine-T method with high radiochemical purity (>98%), it is stable in solution for but of a half life of the radionuclide (8.04 days) The {sup 131} I-anti-CD20 doesn't present dehalogenation in vitro (human serum) during 24 h of incubation at 37 C. According to the tests carried out to establish the immunoreactivity, a percentage of union to cells was obtained (B lymphocytes) bigger to 30%. The biodistribution in mice balb/c one hour after their administration, it shows that there is not high reception in mucous neither kidneys, what indicates that the complex is stable in vivo. In conclusion, the radiopharmaceutical {sup 131} I-anti-CD20 was obtained in sterile injectable solution and free of pyrogens with a radiochemical purity bigger to 98% and a specific activity of 296 MBq. The radiolabelled molecule maintains its biological recognition for the receiving CD20

Hepatotoxicidade Induzida por Fármacos Anti-tuberculosos

Directory of Open Access Journals (Sweden)

Jaime R. Ungo

1998-11-01

Full Text Available RESUMO: Com o objectivo de:Investigar a possível associação entre a infecção pelo virus da Hepatite C (VHC e/ou vírus da Imunodeficiência Humana (VIH com a ocorrência de hepatotoxicidade induzida pela medicação antibacilar.Investigar o possivel papel que o alfa-interferão 2a possa ter. permitindo a re-introdução da terapêutica anti-tuberculosa em doentes com infecção pelo VHC em que se observam clevações recorrentes dos niveis de transaminases enquanto submetidos a medicação com antibacilares.Procedeu-se a um estudo prospectivo em doentes internados no A. G. Holley Hospital com o diagnóstico de tuberculose, no período compreendido entre Dezembro de 1994 e Março de 1996. A população de doentes deste Hospital é essencialmente formada por doentes não aderentes à terapêutica em ambulatório ou doentes em que a existência de patologia associada, problemas psiquiátricos ou sociais interferem com a eficácia terapêutica em ambulatório.Os doentes eram submetidos a uma avaliarção analítica com determinação de ALT, AST, bilirrubina total, serologias para Hepatite A, B, C e VIH e doseamento urinário de drogas. Os exames para avaliação da funrção hepática eram determinados mensalmente após o inicio da medicação e, caso se verificassem altcrarções passavam a ser determinados com periodicidade bissemanal.Todos os doentes com infecção pelo VHC, VIH negativos, que apresentaram elevação recorrente das provas de funrção hepatica (P.F.H. após re-introdução da terapêutica antibacilar, çntraram no protocolo com alfa-interferão. Estes doentes eram submetidos a biópsia hepática e se nesta, se observasse inflamação activa. iniciavam terapêutlca com alfa-interferão. Uma vez que as P.F.H. normalizassem ou se verificasse uma melhoria de pelo menos 50%, sob terapêutica com interferao, re-introduzia-se sequencialmente terapêutica com isoniazida (INH, rifabutina (RBT e pirazinamida (PZA com determina

Dynamics of Anti-Proton -- Protons and Anti-Proton -- Nucleus Reactions

CERN Document Server

Galoyan, A; Uzhinsky, V

2016-01-01

A short review of simulation results of anti-proton-proton and anti-proton-nucleus interactions within the framework of Geant4 FTF (Fritiof) model is presented. The model uses the main assumptions of the Quark-Gluon-String Model or Dual Parton Model. The model assumes production and fragmentation of quark-anti-quark and diquark-anti-diquark strings in the mentioned interactions. Key ingredients of the model are cross sections of string creation processes and an usage of the LUND string fragmentation algorithm. They allow one to satisfactory describe a large set of experimental data, especially, a strange particle production, Lambda hyperons and K mesons.

Analysis of the QQ anti Q anti Q tetraquark states with QCD sum rules

Energy Technology Data Exchange (ETDEWEB)

Wang, Zhi-Gang [North China Electric Power University, Department of Physics, Baoding (China)

2017-07-15

In this article, we study the J{sup PC} = 0{sup ++} and 2{sup ++} QQ anti Q anti Q tetraquark states with the QCD sum rules, and we obtain the predictions M{sub X(cc} {sub anti} {sub c} {sub anti} {sub c,0}{sup {sub +}{sub +)}} =5.99 ± 0.08 GeV, M{sub X(cc} {sub anti} {sub c} {sub anti} {sub c,2}{sup {sub +}{sub +)}} = 6.09 ± 0.08 GeV, M{sub X(bb} {sub anti} {sub b} {sub anti} {sub b,0}{sup {sub +}{sub +)}} = 18.84 ± 0.09 GeV and M{sub X(bb} {sub anti} {sub b} {sub anti} {sub b,2}{sup {sub +}{sub +)}} = 18.85 ± 0.09 GeV, which can be confronted to the experimental data in the future. Furthermore, we illustrate that the diquark-antidiquark type tetraquark state can be taken as a special superposition of a series of meson-meson pairs and that it embodies the net effects. (orig.)

Anti-inflammatory and anti-cancer activity of mulberry (Morus alba L.) root bark

Science.gov (United States)

2014-01-01

Background Root bark of mulberry (Morus alba L.) has been used in herbal medicine as anti-phlogistic, liver protective, kidney protective, hypotensive, diuretic, anti-cough and analgesic agent. However, the anti-cancer activity and the potential anti-cancer mechanisms of mulberry root bark have not been elucidated. We performed in vitro study to investigate whether mulberry root bark extract (MRBE) shows anti-inflammatory and anti-cancer activity. Methods In anti-inflammatory activity, NO was measured using the griess method. iNOS and proteins regulating NF-κB and ERK1/2 signaling were analyzed by Western blot. In anti-cancer activity, cell growth was measured by MTT assay. Cleaved PARP, ATF3 and cyclin D1 were analyzed by Western blot. Results In anti-inflammatory effect, MRBE blocked NO production via suppressing iNOS over-expression in LPS-stimulated RAW264.7 cells. In addition, MRBE inhibited NF-κB activation through p65 nuclear translocation via blocking IκB-α degradation and ERK1/2 activation via its hyper-phosphorylation. In anti-cancer activity, MRBE deos-dependently induced cell growth arrest and apoptosis in human colorectal cancer cells, SW480. MRBE treatment to SW480 cells activated ATF3 expression and down-regulated cyclin D1 level. We also observed that MRBE-induced ATF3 expression was dependent on ROS and GSK3β. Moreover, MRBE-induced cyclin D1 down-regulation was mediated from cyclin D1 proteasomal degradation, which was dependent on ROS. Conclusions These findings suggest that mulberry root bark exerts anti-inflammatory and anti-cancer activity. PMID:24962785

Approach to a Pregnant Woman with Anti D + Anti C Reactivity Pattern: A Diagnostic Conundrum

OpenAIRE

Rai, Preeti; Sharma, Geetika; Singh, Deeksha; Garg, Jyoti

2017-01-01

The Rhesus G antigen is present on all RBCs that are C+ and also on most D+ RBCs. Due to this co-distribution of G with either C or D antigen, it mimics a reactivity pattern of anti C + anti D on Indirect Antiglobulin Test (IAT), though the role of Anti G in causing Hemolytic Disease of Newborn (HDN) is controversial. The differentiation of anti D, anti C, and anti G is essential particularly in pregnant females. We hereby report a rare case of anti G with anti D and anti C in a pregnant woma...

DETERMINAÇÃO DOS VALORES MÉDIOS DAS ENZIMAS AST, DHL, gGT E FAS NO SORO DE EQUINOS SADIOS EM SANTA MARIA, RS

Directory of Open Access Journals (Sweden)

Sônia Terezinha dos Anjos Lopes

1993-12-01

Full Text Available Foram usados 50 eqüinos sadios provenientes do Batalhão de Polícia Montada da Brigada Militar em Santa Maria, RS, sendo 43 machos e 7 fêmeas com idade variadas a partir de 3 anos. Foram colhidos 10ml de sangue da jugular para determinação dos valores da atividade sérica das enzimas aspartato-aminotransferase (AST, desidrogenase lática (DHL, gama-glutamiltransferase (gGT e fosfatase alcalina sérica (FAS. Os resultados encontrados para AST foi de 101 - 190U/I com média de 130UI; DHL foi de 100 - 421 U/l com média de 182U/I; gGT foi de 2 - 27U/I com média de 6.5U/I e FAS foi de 103 - 335U/I com média de 190U/I. A partir de outubro/1992 estes valores passaram a ser referência no laboratório de Patologia Clínica do Hospital de Clinicas Veterinárias da Universidade Federal de Santa Maria.

Especies vegetales empleadas en la insalivación de hojas de “coca” (Erythroxylum coca var. Coca, Erythroxylaceae

Directory of Open Access Journals (Sweden)

Norma I. Hilgert

2000-01-01

Full Text Available Hilgert, N. I. 2000. Especies vegetales empleadas en la insalivación de hojas de “coca”(Erythroxylum coca var. coca, Erythroxylaceae. Darwiniana 38(3-4: 241-252.El estudio se desarrolló en comunidades campesinas de la selva nublada, en la alta cuenca del ríoBermejo, Provincia de Salta, Argentina con el objeto de documentar el uso de plantas involucradas en laelaboración de “yista”. “Yista” es el nombre asignado a las sustancias alcalinas utilizadas durante lainsalivación de hojas de coca (Erythroxylum coca Lam. var. coca. Para la obtención de la “yista” lasplantas seleccionadas deben ser incineradas. Se registraron veintisiete especies nativas y cinco exóticasempleadas, en el presente o en el pasado, para preparar “yista”. Se mencionan los nombres científicos yvulgares y las porciones de la planta utilizadas, y la comunidad donde se registró el uso. Se describe eilustra el modo de preparar “yista”

Partial gastrectomy associated to anterior truncal vagotomy: alterations in metabolism of the calcium. Experimental study in rats Gastrectomia parcial e vagotomia troncular anterior: alterações no metabolismo de cálcio. Estudo experimental em ratos

Directory of Open Access Journals (Sweden)

Elisvânia Freitas dos Santos

2009-06-01

ção. RESULTADOS: Os animais controle apresentaram médias estatísticas maiores (P<0.05 para o consumo de dieta, ganho de peso, cálcio sérico, cálcio urinário, excreção de cálcio nas fezes, absorção aparente de cálcio e atividade da enzima fosfatase alcalina comparados aos animais do grupo gastrectomizado. Entretanto, a concentração de cálcio ósseo foi superior no grupo de animais gastrectomizados (P<0,05 quando comparado com o grupo controle. CONCLUSÃO: A gastrectomia parcial associada à vagotomia troncular anterior mostrou ser um bom modelo experimental para estudo com o metabolismo de cálcio, ocasionando diminuição da absorção de cálcio, cálcio sérico, cálcio urinário e fosfatase alcalina. No entanto, para alterações a nível ósseo em ratos, sugere-se um estudo experimental em período maior.

Vulnerability and self-perceived health status among light and heavy smokers: the relationship to short-term fear appeal tobacco control messages Vulnerabilidade e percepção de saúde auto-referida entre fumantes leves e pesados: a relação com mensagens anti-fumo voltadas para o apelo ao medo imediato

Directory of Open Access Journals (Sweden)

André Salem Szklo

2009-07-01

Full Text Available It is important to stimulate smokers to acquire some level of risk perception associated with their current behavior in order to motivate smoking cessation. The present article attempts to understand how the content of short-term fear appeal government tobacco messages may interact with different levels of daily cigarette consumption in order to affect smokers' vulnerabilities, expressed by self-perceived health status. A Poisson model was used to estimate the prevalence ratio of fair or poor self-perceived health status (FPHS according to daily cigarette consumption. We also calculated the proportions of smokers who stated that selected health warning pictures on cigarette packets encourage people to quit smoking, stratified by self-perceived health status and daily cigarette consumption. The proportion of smokers with FPHS was 25% higher among those who smoked > 20 cigarettes/day (p = 0.01. Among smokers with FPHS, heavy smokers showed the highest proportions of responses in favor of selected warning pictures most closely related to losses in ordinary daily living, such as shortness of breath and being bothered by cigarette addiction. Short-term loss-framed tobacco control messages seem to have raised awareness of vulnerability among heavier smokers.É importante incentivar os fumantes a adquirir algum nível de percepção do risco associado ao seu comportamento atual, para motivá-los a parar de fumar. O artigo procura elucidar em que medida o conteúdo do apelo ao medo imediato contido em mensagens anti-fumo nas campanhas governamentais pode interagir com o consumo diário de cigarros, no sentido de afetar as vulnerabilidades dos fumantes, expressas pela percepção de saúde auto-referida. Um modelo de Poisson foi utilizado para estimar a razão de prevalências de percepção de saúde auto-referida regular ou ruim, segundo consumo diário de cigarros. Calculou-se também a proporção de fumantes que afirmou que mensagens anti

Novos anti-histamínicos: uma visão crítica New antihistamines: a critical view

Directory of Open Access Journals (Sweden)

Inês Cristina Camelo-Nunes

2006-11-01

Full Text Available OBJETIVO: Avaliar criticamente os mais novos anti-histamínicos anti-H1 e os diferentes termos utilizados para denominá-los, com base na revisão de evidências sobre o papel dos anti-H1 no tratamento das doenças alérgicas. FONTES DOS DADOS: Artigos originais, revisões e consensos indexados nos bancos de dados MEDLINE e PUBMED de 1998 a 2006. Palavra chave: anti-histamínicos. SÍNTESE DOS DADOS: Os anti-histamínicos de segunda geração diferenciam-se dos de primeira geração por sua elevada especificidade e afinidade pelos receptores H1 periféricos e pela menor penetração no sistema nervoso central (SNC, com conseqüente redução dos efeitos sedativos. Embora os anti-histamínicos de segunda geração sejam, geralmente, melhor tolerados do que seus predecessores, alguns efeitos adversos, principalmente cardiotoxicidade, surgiram com alguns deles. Nos últimos 20 anos, novos compostos, com diferentes farmacocinéticas, foram sintetizados. A maioria deles manifesta propriedades antiinflamatórias que independem de sua atividade no receptor H1. Aprimoramentos mais recentes, geralmente na forma de metabólitos ativos, levaram ao uso do termo anti-histamínico de terceira geração. Esse termo surgiu espontaneamente, sem uma descrição clara de seu significado e implicações clínicas, criando grande confusão entre os profissionais da saúde. CONCLUSÕES: Com base nas evidências sobre anti-histamínicos anti-H1, nenhum deles pode ser considerado como "anti-histamínico de terceira geração". Para tanto, seria preciso comprovar que a nova classe de anti-histamínicos possui vantagens clínicas distintas sobre os compostos existentes e preenche pelo menos três pré-requisitos: ausência de cardiotoxicidade, de interações medicamentosas e de efeitos sobre o SNC.OBJECTIVE: To perform a critical evaluation of the more recent H1 antihistamines and the various terms used to describe them, based on a review of evidence on their role in

Projeto do sistema anti-ressonante da fiação dos transdutores para o detector Mario Schenberg

Science.gov (United States)

Vieira, S. J.., Jr.; Melo, J. L.

2003-08-01

O detector de ondas gravitacionais Mario Schenberg está sendo projetado e construído pelo grupo Gráviton. Sua construção está ocorrendo no Laboratório de Estado Sólido e Baixas Temperaturas (LESBT) da Universidade de São Paulo, na cidade de São Paulo. Esse detector possui uma massa ressonante esférica de cobre-alumínio, com 65 cm de diâmetro, pesando aproximadamente 1150 Kg, suspensa por um sistema de isolamento vibracional, que se encontra em fase de testes preliminares. A real eficácia desse sistema, entretanto, só poderá ser comprovada quando o detector estiver aparelhado com, pelo menos, um transdutor eletromecânico de altíssima sensibilidade acoplado à massa ressonante. Neste momento, não só este sistema de isolamento vibracional será posto em teste, como o do projeto da fiação que transporta os sinais de microondas até os transdutores e destes para a pré-amplificação. Apesar dessa fiação ter sido projetada para não apresentar nenhum contato com a superfície esférica da antena, de maneira a não haver nenhuma transmissão de ruído vibracional do laboratório para esta, deve-se minimizar o ruído microfônico produzido nessa fiação por oscilações mecânicas, uma vez que ela não utiliza nenhum sistema de isolamento vibracional. Com o intuito de resolver este problema, projetamos uma estrutura, formada por pequenos cilindros conectados por barras, a qual não terá nenhuma ressonância mecânica na faixa de freqüências de interesse para detecção (3000 - 3400 Hz). Desta forma, as vibrações nessa faixa não serão amplificadas. O projeto foi feito usando iterativamente, de maneira a otimizar os resultados obtidos, o programa de elementos finitos Msc/Nastran. Através de simulações feitas neste programa, determinamos os parâmetros geométricos ideais a serem utilizados, os quais proporcionam a maior região espectral de interesse livre de ressonâncias.

Specificity of anti-phospholipid antibodies in infectious mononucleosis: a role for anti-cofactor protein antibodies

Science.gov (United States)

Sorice, M; Pittoni, V; Griggi, T; Losardo, A; Leri, O; Magno, M S; Misasi, R; Valesini, G

2000-01-01

The antigen specificity of anti-phospholipid antibodies in infectious mononucleosis (IM) was studied using ELISA for the detection of anti-β2-glycoprotein I (β2-GPI), anti-annexin V, anti-protein S and anti-prothrombin antibodies and TLC immunostaining for the detection of anti-phospholipid antibodies. This technique enabled us to look at antibodies reacting to ‘pure’ phospholipid antigens in the absence of protein contamination. Sera from 46 patients with IM, 18 with systemic lupus erythematosus (SLE), 21 with primary anti-phospholipid antibody syndrome (PAPS), 50 with Helicobacter pylori infection and 30 healthy blood donors were tested. This study highlights anti-phospholipid antibodies in patients with IM as specific ‘pure’ anti-cardiolipin antibodies, while in PAPS and SLE patients anti-phosphatidylserine and anti-phosphatidylethanolamine antibodies were also found. This investigation also shows that the anti-cardiolipin antibodies found in IM can be present with anti-cofactor protein antibodies. The higher prevalence of anti-cofactor antibodies found in IM sera than in Helicobacter pylori sera may be due to the immunostimulatory effect and/or the polyclonal activation often observed in course of Epstein–Barr virus infection. However, anti-β2-GPI and, to a lesser extent, anti-prothrombin antibodies occur with a significantly lower prevalence in IM than in PAPS patients. This finding suggests that these antibodies should be regarded as the expression of the broad autoimmune syndrome involving the phospholipid-binding plasma proteins. PMID:10792380

One single dose of 200 microg of antenatal RhIG halves the risk of anti-D immunization and hemolytic disease of the fetus and newborn in the next pregnancy.

Science.gov (United States)

Koelewijn, Joke M; de Haas, Masja; Vrijkotte, Tanja G M; Bonsel, Gouke J; van der Schoot, C Ellen

2008-08-01

The objective was the evaluation of the effect of the Dutch national routine antenatal RhIG (anti-D) immunization prevention (RAADP) program comprising one single dose of 200 microg (1000 IU) of RhIG in the 30th week of pregnancy, restricted to women without a living child. A nationwide historic control study was performed. All newly detected anti-D-immunized para-1 in 1999, 2002, and 2004 were included and classified on the basis of received prophylaxis during the first pregnancy: antenatal and postnatal versus only postnatal RhIG. The numbers of D- parae-1 who delivered a D+ first child before the introduction (control group) or after the introduction (intervention group) of the RAADP were calculated from Vital Birth Statistics (8,700 and 12,000, respectively). Fifty-eight newly detected anti-D immunizations in the first trimester were observed in the control group and 39 in the intervention group, which resulted in a significant reduction of the prevalence of new anti-D immunizations from 0.67 percent (95% confidence interval [CI], 0.50%-0.84%) to 0.31 percent (95% CI, 0.21%-0.41%). No reduction was observed in anti-D immunizations newly detected at the 30th-week screening (0.25%). A nonsignificant risk reduction of the risk of severe hemolytic disease of the fetus and newborn (HDFN) was found (0.23% vs. 0.10%). The numbers needed to treat to prevent one anti-D-immunized pregnancy and one case of subsequent severe HDFN were 357 and 1255, respectively. RAADP of one single dose of 200 microg of RhIG in addition to postnatal RhIG (200 microg) halves the risk of anti-D immunization and subsequent severe HDFN.

Anti-inflammatory and anti-oxidant activities of Secamone afzelii ...

African Journals Online (AJOL)

. This study re-ports the anti-inflammatory and antioxidant properties of S. afzelii. The anti-inflammatory activity was determined by the carrageenan-induced paw oedema method in 7 day old chicks and antioxi-dant property by the 2 ...

The Meaning of Anti-Americanism: A Performative Approach to Anti-American Prejudice

Directory of Open Access Journals (Sweden)

Felix Knappertsbusch

2013-06-01

Full Text Available A contribution to the ongoing debate on how anti-Americanism can be adequately conceptualized and how such prejudice can be distinguished from legitimate criticism, arguing that part of these conceptual problems arise from a too narrow focus on defining anti-Americanism and the use of standardized empirical operationalizations. Such approaches exhibit severe limitations in grasping the flexibility of the phenomenon in everyday discourse and often underestimate or ignore the interpretive aspect involved in identifying utterances as anti-American prejudice. Alternatively, a performative approach is proposed, understanding anti-Americanism as a network of speech acts bound by family resemblance rather than identical features. In combination with qualitative empirical research methods such a conceptualization is especially suited to account for the flexible, situated use of anti-American utterances. At the same time it grants reflexivity to the research concept, in the sense of a close description of the scientific application of the notion of anti-Americanism. Two empirical examples from an interview study on anti-American speech in Germany illustrate the potential of such an approach, providing an insight into how anti-Americanism is incorporated into the construction and expression of racist and revisionist national identifications in everyday discourse.

Impact of leptonic tau decays on the distribution of anti B → Dμ anti ν

Energy Technology Data Exchange (ETDEWEB)

Bordone, Marzia; Isidori, Gino; Dyk, Danny van [Physik-Institut, Universitaet Zuerich, Winterthurer Strasse 190, 8057 Zuerich (Switzerland)

2016-07-01

We present results for the decay rate of anti B → Dτ(→ μ anti νν) anti ν, which is a sizeable background to anti B → Dμ anti ν decays. In particular, we calculate analytically the differential decay rate with respect to all seven kinematic variables. We consider the impact of this decay on the distributions of anti B → Dμ anti ν in either the muon helicity angle or the muon energy in the anti B rest frame. Our numerical results are obtained from Monte Carlo pseudo events. Finally, we provide necessary information for cross checks of the experimental analyses.

Synthesis and characterization of anti-bacterial and anti-fungal citrate-based mussel-inspired bioadhesives

Science.gov (United States)

Guo, Jinshan; Wang, Wei; Hu, Jianqing; Xie, Denghui; Gerhard, Ethan; Nisic, Merisa; Shan, Dingying; Qian, Guoying; Zheng, Siyang; Yang, Jian

2016-01-01

Bacterial and fungal infections in the use of surgical devices and medical implants remain a major concern. Traditional bioadhesives fail to incorporate anti-microbial properties, necessitating additional anti-microbial drug injection. Herein, by the introduction of the clinically used and inexpensive anti-fungal agent, 10-undecylenic acid (UA), into our recently developed injectable citrate-based mussel-inspired bioadhesives (iCMBAs), a new family of anti-bacterial and anti-fungal iCMBAs (AbAf iCs) was developed. AbAf iCs not only showed strong wet tissue adhesion strength, but also exhibited excellent in vitro cyto-compatibility, fast degradation, and strong initial and considerable long-term anti-bacterial and anti-fungal ability. For the first time, the biocompatibility and anti-microbial ability of sodium metaperiodate (PI), an oxidant used as a cross-linking initiator in the AbAf iCs system, was also thoroughly investigated. Our results suggest that the PI-based bioadhesives showed better anti-microbial properties compared to the unstable silver-based bioadhesive materials. In conclusion, AbAf iCs family can serve as excellent anti-bacterial and anti-fungal bioadhesive candidates for tissue/wound closure, wound dressing, and bone regeneration, especially when bacterial or fungal infections are a major concern. PMID:26874283

Aportaciones al estudio de la actividad antimicrobiana de los antisépticos y desinfectantes

OpenAIRE

Hernández Rodríguez, Águeda

2006-01-01

Consultable des del TDX Títol obtingut de la portada digitalitzada Las infecciones nosocomiales (IN) provocan una elevada morbilidad y mortalidad, una gran carga de trabajo para el personal y un elevado coste económico. El uso eficaz de los biocidas, antisépticos y desinfectantes, junto con la desinfección de los dispositivos médicos y de superficies, el lavado de manos y las técnicas de barrera, constituyen las medidas de prevención de dichas IN. El desarrollo de estudios sobre la acti...

Detecção sorológica de anti-HPV 16 e 18 e sua associação com os achados do papanicolaou em adolescentes e mulheres jovens Serological detection of anti HPV 16/18 and its association with pap smear in adolescents and young women

Directory of Open Access Journals (Sweden)

Cristina Helena Rama

2006-02-01

Full Text Available OBJETIVO: Verificar a taxa de anticorpos neutralizantes anti-HPV 16 e/ou 18, e a sua associação com os achados da citologia oncológica do colo uterino em adolescentes e mulheres jovens. MÉTODOS: Foram incluídas, neste estudo transversal, 541 mulheres de 15 a 25 anos de idade, saudáveis, sexualmente ativas, que apresentaram exame ginecológico normal, no período de setembro a novembro de 2000. Foi obtida uma amostra cervical para citologia em meio líquido e uma amostra de sangue para identificação dos anticorpos anti-HPV 16 e/ou 18, por meio do método ELISA. As amostras foram encaminhadas para um laboratório de referência na Bélgica. Para análise estatística, foram estimadas a prevalência e a razão de prevalência (RP, com intervalo de confiança de 95%. RESULTADOS: Entre as mulheres incluídas, 150 (27,7% apresentaram resultados positivos da sorologia sendo: 79 (14,6% por anticorpos anti-HPV 16, 35 (6,4% anti-HPV 18 e 36 (6,6% anti-HPV 16 e 18. Foram detectadas anormalidades citológicas em 107 casos (20,5%, sendo 63 classificadas como atipias celulares de significado indeteminado (ASCUS, 41 casos sugestivos de lesões de baixo (LSIL e três casos sugestivos de lesões de alto grau (HSIL. A prevalência de citologias anormais quando a sorologia foi positiva foi apenas 1,75 vez a prevalência de citologias positivas observadas com sorologia negativa. Nesta amostra não houve evidência de associação entre os resultados anormais da citologia e a positividade da sorologia. CONCLUSÃO: O resultado deste estudo indica uma alta prevalência de sorologia positiva para o HPV 16 e 18 em mulheres jovens sadias sem relação com os achados anormais da colpocitologia.OBJECTIVE: Verify the relation between HPV 16/18 antibodies and the association with cervical cytology findings in adolescents and young women. METHODS: A cross sectional study with 541 healthy and sexually active women from 15 to 25 years of age was carried on from

Aproveitamento de resíduos da agroindústria do óleo de dendê para a produção de lipase por Aspergillus Níger

Directory of Open Access Journals (Sweden)

Edmar das Mercês Penha

2015-01-01

Full Text Available RESUMO: Neste trabalho, foi avaliado o desempenho de duas linhagens de Aspergillus niger (mutante 11T53A14 e selvagem C previamente selecionadas como promissoras para a produção de lipases, utilizando meios de cultivo formulados a partir da torta de dendê (palmiste e da borra alcalina do refino do óleo de dendê (borra de dendê, resíduos provenientes da agroindústria do óleo de dendê (palma por fermentação em estado sólido (FES. Os experimentos de produção da enzima em FES foram conduzidos em colunas aeradas, incubadas em banho-maria a 32ºC com entrada controlada de ar não umedecido de 1,0vvm. Os melhores resultados foram obtidos com a cepa mutante A. niger 11T53A14 em meio contendo torta de dendê umedecida com solução sulfato de amônio (1,2% e com a adição de 3% da borra de dendê. O valor máximo da atividade da lipase neste meio foi de 72,57U gss-1 em 48 horas. Esse valor foi 47,5% superior ao obtido no meio sem a borra de dendê. A comparação do desempenho da cepa mutante com a cepa selvagem mostrou que o meio composto por torta de dendê adicionado com sulfato de amônio e borra de dendê induziu ambas as linhagens a produzir lipases com bons níveis de atividade, além de reduzir o tempo de processo de fermentação.

Para neoplastic cataplexy: evolution of PET with [{sup 18}F] -F.D.G. imaging; Cataplexie paraneoplasique: evolution de l'imagerie TEP au [18F]-FDG

Energy Technology Data Exchange (ETDEWEB)

Farida, K.; Fernandez, P.; Guyot, M.; Jeandot, R.; Allard, M. [Service de medecine nucleaire, CHU de Bordeaux, (France); Sibon, I. [service de neurologie, CHU de Bordeaux, (France)

2009-05-15

Cataplexy is sudden and transient episode of loss of muscle tone, often triggered by emotions. We find it in the Gelineau disease but it can be constitutes an exceptional clinical expression of para-neoplasic syndromes. We report the evolution of the PET imaging for a patient during a para-neoplasic syndrome secondary to a testes teratoma associated to the presence of antibodies anti-Ma2. Different aspects of the {sup 18}F-F.D.G. fixation can be observed during the para-neoplasic syndromes affecting the central nervous system. Their values in term of prognosis and help to the therapy decision are still to define. (N.C.)

Características testiculares de touros imunizados com vacina anti-hormônio liberador do hormônio luteinizante Testicular characteristics of bulls immunosterilized with anti-luteinizing hormone-releasing hormone vaccine

Directory of Open Access Journals (Sweden)

Ricardo Zanella

2009-10-01

Full Text Available O objetivo deste trabalho foi avaliar a ação imunoesterilizadora de uma vacina anti-hormônio liberador de hormônio luteinizante (LHRH, composta por ovalbumina-LHRH-7 e tiorredoxina-LHRH-7, em touros mestiços Nelore. Vinte e seis touros, com dois anos de idade, foram distribuídos aleatoriamente em dois grupos de 13 animais. No grupo I, os animais receberam uma dose e dois reforços da vacina nos dias 0, 141, e 287 do experimento. No grupo II, os animais não receberam nenhum tratamento (controle. Para avaliar o efeito da vacina nos touros, foi realizada a mensuração da circunferência escrotal no início do experimento e no dia do abate, 741 dias depois. Por ocasião do abate, também foi coletada uma amostra dos testículos para avaliação histológica. O grupo imunizado apresentou circunferência escrotal ao abate de 22±5,98 cm, menor do que a do grupo controle que foi de 35,6±2,4 cm. Na análise histológica dos animais do grupo imunizado, foi observada degeneração testicular com ausência de espermatozoides em 85% dos animais avaliados, os outros 15% apresentaram redução no número de espermatozoides, em comparação aos animais do grupo controle. A vacina anti-LHRH, com fusão de proteínas, é efetiva na castração imunológica de touros e deve ser considerada como alternativa para utilização na produção bovina extensiva no Brasil.The objective of this study was to evaluate the immunosterilization action of the anti-luteinizing hormone-releasing hormone (LHRH vaccine, composed with ovalbumin-LHRH-7 and thioredoxin-LHRH-7, in Nelore-cross bulls. Twenty-six 2-year old bulls were randomly assigned in two groups of 13 animals each. The animals of group I received a primary and two booster injections of the vaccine on days 0, 141, and 287 of the experiment. In group II, the control group, the bulls did not receive any type of treatment. Scrotal circumference was measured in the beginning of the experiment and at slaughter

Biological activities (anti-inflammatory and anti-oxidant) of fractions ...

African Journals Online (AJOL)

Biological activities (anti-inflammatory and anti-oxidant) of fractions and methanolic extract of Philonotis hastate (Duby Wijk & MargaDant). ... The fractions and methanolic extract exhibited moderate antioxidant potentials with various models. The flavonoid contents of the methanol extract and fractions ranged between 1.70 ...

PROSPECÇÃO TECNOLÓGICA: PROTETORES SOLARES ANTI UVA E ANTI UVB

Directory of Open Access Journals (Sweden)

Douglas Dourado Oliveira

2014-03-01

Full Text Available Com o aumento da temperatura no planeta devido o efeito estufa, há uma preocupação com as radiações ultravioletas que incidem na pele dos seres humanos. Diante disso a indústria cosmética vem desenvolvendo constantemente formulações contendo filtros solares, anti UVA, anti UVB e também os dois filtros associados numa mesma formulação, retardando o envelhecimento cutâneo, formação dos radicais livres e prevenindo uma grande problemática atual, o câncer de pele. O objetivo deste trabalho foi realizar uma prospecção para conhecimento do desenvolvimento científico e tecnológico relacionado à aplicação de filtros solares associados em uma mesma formulação baseando-se no número de patentes depositadas e de artigos publicados. A prospecção foi realizada no Banco Europeu de Patentes (EPO, no Banco Americano de Marcas e Patentes (USPTO e no Banco de dados do Instituto Nacional de Propriedade Industrial do Brasil (INPI, bem como nas bases de periódicos, CAPES, Pubmed, Science Direct e ACS Publications. O maior número de patentes foi depositado nos últimos 5 anos. As patentes em sua maioria estão classificadas em A61K. Os responsáveis por maior número de patentes foram os Estados Unidos, China e Japão. Há uma diminuição no número de depósitos de patentes quando se associou a UVA e UVB no fotoprotetor. Em relação à produção científica o número de publicações referente a formulações fotoprotetoras contendo os dois filtros associados é maior que o de depósito de patentes, porém segue o mesmo perfil de redução ao associar os filtros numa mesma formulação.

Ensaios clínicos toxicológicos, fase I, de um fitoterápico composto (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill

Directory of Open Access Journals (Sweden)

Patrícia Trindade C. Paulo

Full Text Available Neste estudo, foram realizados ensaios clínicos toxicológicos, fase I, do produto fitoterápico composto pelas plantas medicinais Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill. O estudo foi realizado no Hospital Universitário Lauro Wanderley/UFPB/PB e, para isto, foram selecionados 28 voluntários sadios, sendo 14 homens e 14 mulheres que ingeriram por via oral, ininterruptamente durante 8 semanas, 15 mL do produto, três vezes ao dia; e no 3º e 7º dia, 3ª e 6ª semanas e 24 h após a 8ª semana, foram feitas avaliações clínicas e laboratoriais para análise da toxicidade aguda e crônica. Os resultados obtidos demonstraram que os pacientes não apresentaram alterações clínicas, laboratoriais e reações adversas significantes, apenas pequenas alterações foram detectadas no sangue através da aspartato transaminase (AST e fosfatase alcalina no grupo feminino para um p < 0,05; no entanto, estes valores determinados permaneceram dentro dos valores de normalidade para indivíduos adultos. Conclui-se que estes dados, em complementação àqueles obtidos com os estudos pré-clínicos, confirmam a baixa toxicidade do produto fitoterápico.

Experimental Study on Anti-body effects of Anti-BV on the Bee Venom Herbal Acupuncture

Directory of Open Access Journals (Sweden)

Ki Rok Kwon

2005-02-01

Full Text Available Objectives : To observe physiological anti-body effects of anti-BV, acute toxic response, measurement of LD50, and the effects of anti-body were evaluated. Methods : LD50 of Anti-Bee Venom were measured, and to analyze acute toxic responses, weight, and the anti-body effects various concentrations of Anti-BV were diluted and the survival rate was measured. Cell blood count (CBC, liver, spleen, and kidney pathologies were observed from the histological aspects. Results : Experiment was conducted to observe Anti-BV as the anti-body to the bee venom and the following results were obtained: 1. Anti-BV was injected intraperitoneally and no toxic responses were witnessed. All of the experiment subjects stayed alive during the experiment, making LD50 analysis impossible. 2. Anti-BV was injected intraperitoneally in mice and no significant weight changes were measured between the control group and the experiment groups. 3. Measuring the concentration dependent survival rate, the highest survival rate was at the concentration of 1.25×102mg/kg(1/2.000 for Anti-BV. 4. No particular results were shown in the CBC test. 5. Observation of changes in the organ tissues, Anti-BV was found to suppress blood stasis in the liver and inhibit necrosis of the cells. Conclusion : Above results suggest that Anti-BV doesn't cause any toxic responses in the body and works as an anti-body to the bee venom. Further studies must be followed to secure the findings.

Preparation of the radiopharmaceutical {sup 131}I-Anti-CD20 for the treatment of lymphomas; Preparacion del radiofarmaco {sup 131}I-Anti-CD20 para el tratamiento de linfomas

Energy Technology Data Exchange (ETDEWEB)

Pantoja H, I.E

2004-07-01

At the present time they are considered to the lymphomas like a problem of first magnitude since has happened it is necessary to be the fifth cancer cause in the world. Different treatments focused to the lymphoma like the chemotherapy and the radiotherapy, have been employees to counteract the No-Hodgkin lymphoma, without these they don't exclude the healthy tissue of the toxicity. It is for it that is taking a new direction with the employment of the directed radioimmunotherapy since this it allows to kill wicked cells selectively with radiation dose joined to the apoptosis and cytotoxicity induced by the own one bio molecule. The radioimmunotherapy with radiolabelled antibodies directed to the surface antigen CD20 represents a new modality for the treatment of No-Hodgkin lymphoma and potentially other illnesses. In this work the parameters of optimization are presented for the preparation, control of quality and evaluation of the stability in vitro and in vivo of the monoclonal antibody anti-CD20 labelled with {sup 131} I for the treatment of No-Hodgkin lymphoma. The anti-CD20 labelled by the chloramine-T method with high radiochemical purity (>98%), it is stable in solution for but of a half life of the radionuclide (8.04 days) The {sup 131} I-anti-CD20 doesn't present dehalogenation in vitro (human serum) during 24 h of incubation at 37 C. According to the tests carried out to establish the immunoreactivity, a percentage of union to cells was obtained (B lymphocytes) bigger to 30%. The biodistribution in mice balb/c one hour after their administration, it shows that there is not high reception in mucous neither kidneys, what indicates that the complex is stable in vivo. In conclusion, the radiopharmaceutical {sup 131} I-anti-CD20 was obtained in sterile injectable solution and free of pyrogens with a radiochemical purity bigger to 98% and a specific activity of 296 MBq. The radiolabelled molecule maintains its biological recognition for the receiving

Anti-idiotypes against a monoclonal anti-haloperidol antibody bind to dopamine receptor

International Nuclear Information System (INIS)

Elazar, Z.; Kanety, H.; Schreiber, M.; Fuchs, S.

1988-01-01

Anti-idiotypic antibodies were raised in rabbits by immunization with a monoclonal anti-haloperidol antibody. Some of these anti-idiotypic antibodies bind in a concentration dependent manner to bovine striatal membranes. Following affinity purification, these antibodies inhibit haloperidol binding to striatal membranes and deplete [ 3 H]-spiperone binding sites from a solubilized preparation of striatal membranes. It is thus concluded that these anti-idiotypic antibodies are an internal image of haloperidol and as such can interact with D 2 -dopamine receptors

Photon anti bunching versus phantom anti bunching?

International Nuclear Information System (INIS)

Miranowicz, A.; Ekert, A.; Ekert, A.; Bajer, J.; Leonski, W.

1997-01-01

Photon anti bunching defined by two-time correlation functions has hitherto, to our best knowledge, been considered to constitute a unique, well defined effect. We show explicitly that this is by no means the case. We analyze two of the most famous definitions showing that both anti bunching and bunching effects according to one definition can be accompanied by arbitrary photon correlation effects according to another. As an example we discuss a model of parametric frequency conversion. (authors)

Analgesic, anti-inflammatory and anti-platelet activities of Buddleja crispa.

Science.gov (United States)

Bukhari, Ishfaq A; Gilani, Anwar H; Meo, Sultan Ayoub; Saeed, Anjum

2016-02-25

Buddleja crispa Benth (Buddlejaceae) is a dense shrub; several species of genus Buddleja have been used in the management of various health conditions including pain and inflammation. The present study was aimed to investigate the analgesic, anti-inflammatory and anti-platelet properties of B. crispa. Male rats (220-270 gm,) and mice (25-30 gm) were randomly divided into different groups (n = 6). Various doses of plant extract of B. crispa, its fractions and pure compounds isolated from the plant were administered intraperitoneally (i.p). The analgesic, anti-inflammatory and anti-platelet activities were assessed using acetic acid and formalin-induced nociception in mice, carrageenan-induced rat paw edema and arachidonic acid-induced platelets aggregation tests. The intraperitoneal administration of the methanolic extract (50 and 100 mg/kg), hexane fraction (10 and 25 mg/kg i.p) exhibited significant inhibition (P < 0.01) of the acetic acid-induced writhing in mice and attenuated formalin-induced reaction time of animals in second phase of the test. Pure compounds BdI-2, BdI-H3 and BH-3 isolated from B. crispa produced significant (P < 0.01) analgesic activity in acetic acid-induced and formalin tests. The crude extract of B. crispa (50-200 mg/kg i.p.) and its hexane fraction inhibited carrageenan-induced rat paw edema with maximum inhibition of 65 and 71% respectively (P < 0.01). The analgesic and anti-inflammatory effect of the plant extract and isolated pure compounds were comparable to diclofenac sodium. B. crispa plant extract (0.5-2.5 mg/mL) produced significant anti-platelet effect (P < 0.01) with maximum inhibition of 78% at 2.5 mg/ml. The findings from our present study suggest that B. crispa possesses analgesic, anti-inflammatory and anti-platelet properties. B. crispa could serve a potential novel source of compounds effective in pain and inflammatory conditions.

Efecto hepatoprotector del extracto hidroetanólico atomizado del maíz morado (Zea mays L. en lesiones hepáticas inducidas en ratas

Directory of Open Access Journals (Sweden)

Renán Hañari-Quispe

2015-04-01

Full Text Available Objetivos: Evaluar el efecto hepatoprotector del extracto hidroetanólico atomizado de Zea mays variedad morado sobre lesiones hepáticas inducidas en ratas. Diseño: Experimental. Institución: Laboratorio de Farmacología Experimental, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Extracto hidroetanólico atomizado de maíz morado (EAM. Intervenciones: Se formó seis grupos de ratas machos (n=10, por cada grupo, se les administró fenobarbital a concentración de 0,5 g/L en agua potable ad líbitum por 15 días; posteriormente se administró tetracloruro de carbono (CCl a una dosis de 0,2 mL/kg, por vía oral. El diseño experimental fue el siguiente: G1: suero fisiológico (SSF; G2: CCl0,2 mL/kg (T;4G3: T+ 4 silimarina 25 mg/kg; G4: T + EAM 500 mg/kg; G5: T + EAM 1 000 mg/kg; G6: T + EAM 2 000 mg/kg. Principales medidas de resultados: Perfil hepático, especies reactivas al ácido tiobarbitúrico (TBARS en suero, índice hepático, observación histológica. Resultados: Se observó aumento significativo (p<0,05 en la actividad de alanina amino transferasa (ALT entre el grupo G2 y los grupos G3 y G4 (p<0,001. Hubo disminución significativa (p<0,05 de fosfatasa alcalina (FAL en el grupo G2 con respecto G1. El nivel de TBARS fue menor en el grupo que recibió 1 000 mg/kg de EAM con respecto al control. Las actividades de HDL-C y triglicéridos no mostraron diferencias significativas. Se ha observado la reducción de 60% de la lesión hepática, evidenciado con menor daño del hepatocito al estudio histológico. Conclusiones: El extracto hidroetanólico atomizado de maíz morado a la dosis de 1 000 mg/kg disminuyó las lesiones hepáticas inducidas en ratas.

Efecto hepatoprotector del extracto acuoso de Smallanthus sonchifolius (yacón en un modelo de intoxicación con acetaminofén

Directory of Open Access Journals (Sweden)

Acela Inés Arnao-Salas

2012-07-01

Full Text Available En la medicina tradicional se ha publicado que las hojas de Smallanthus sonchifolius (yacón poseen efectos antidiabético y hepatoprotector. Objetivos: Evaluar en suero y hematíes el efecto hepatoprotector del extracto acuoso de hojas de yacón (EAY en un modelo de intoxicación con acetaminofén en ratas. Diseño: Experimental, transversal. Institución: Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Hojas de yacón. Intervenciones: Se formó cinco grupos de ratas hembra (n=6 que recibieron por cinco días, por vía oral, suero fisiológico (SF, EAY o silimarina (Sil (50 mg/kg y luego de 1 hora, SF o acetaminofén (A 250 mg/kg, según lo siguiente: G1 (control; SF-SF, G2 (SF-A, G3 (EAY (200 mg/kg-A, G4 (EAY (400 mg/kg-A y G5 (Sil-A. Principales medidas de los resultados: Actividad de aspartato amino transferasas (AST, alanina amino transferasa (ALT, fosfatasa alcalina (FAL γ γ-amino transferasa (γ-GTP; niveles de bilirrubina total (BT, proteνnas y lipoperoxidaciσn (MDA. En hematíes, actividades de superóxido dismutasa (SOD, catalasa (CAT y hemoglobina. Resultados: Se observó aumento significativo (p<0,05 en la actividad de γ-GTP entre el grupo G2 y los grupos G3 y G4. Hubo disminuciσn significativa (p<0,05 de proteνnas en el grupo G2 con respecto G1. El nivel de MDA fue menor en el grupo que recibió 200 mg/kg de EAY con respecto al control. Las actividades de AST, ALT y FAL no mostraron diferencias significativas. La relación SOD/CAT fue similar entre los grupos G1, G4 y G5, evidencia de una recuperación del daño causado por el acetaminofén. Conclusiones: La administración del EAY tuvo un efecto hepatoprotector comparable a la silimarina.

Preparation, spectroscopic, thermal, antihepatotoxicity, hematological parameters and liver antioxidant capacity characterizations of Cd(II), Hg(II), and Pb(II) mononuclear complexes of paracetamol anti-inflammatory drug

Science.gov (United States)

El-Megharbel, Samy M.; Hamza, Reham Z.; Refat, Moamen S.

2014-10-01

Keeping in view that some metal complexes are found to be more potent than their parent drugs, therefore, our present paper aimed to synthesized Cd(II), Hg(II) and Pb(II) complexes of paracetamol (Para) anti-inflammatory drug. Paracetamol complexes with general formula [M(Para)2(H2O)2]·nH2O have been synthesized and characterized on the basis of elemental analysis, conductivity, IR and thermal (TG/DTG), 1H NMR, electronic spectral studies. The conductivity data of these complexes have non-electrolytic nature. Comparative antimicrobial (bacteria and fungi) behaviors and molecular weights of paracetamol with their complexes have been studied. In vivo the antihepatotoxicity effect and some liver function parameters levels (serum total protein, ALT, AST, and LDH) were measured. Hematological parameters and liver antioxidant capacities of both Para and their complexes were performed. The Cd2+ + Para complex was recorded amelioration of antioxidant capacities in liver homogenates compared to other Para complexes treated groups.

Polysaccharides from Sargassum thunbergii: Monthly variations and anti-complement and anti-tumour activities.

Science.gov (United States)

Jin, Weihua; Liu, Ge; Zhong, Weihong; Sun, Chaomin; Zhang, Quanbin

2017-12-01

Monthly variations of polysaccharides from Sargassum thunbergii and their anti-complement and anti-tumour activities were investigated. It was observed that an increase in fucose and total sugar contents occurred during the growth period (from early April to mid-June), accompanied by a decrease in molar ratios of other monosaccharides to fucose. The highest yields were obtained from early July to early September, which was in accordance with the significant increase in molar ratio of glucose to fucose and decrease in molar ratio of other monosaccharides to fucose. And the above results suggested that S. Thunbergii synthesized large amount of laminaran, the storage substance of brown algae, during the senescence period. However, sulfate contents were relatively stable in the life cycle of S. thunbergii. These results suggested that S. thunbergii synthesized complex sulfated heteropolysacchairdes during inactive period, while during other periods, it synthesized more sulfated galactofucan. All polysaccharides showed anti-complement activity, suggesting that the harvesting time did not influence the anti-complement activities. In the anti-tumour assay in vitro, the polysaccharides taken during the senescence period had much lower anti-tumour activity, suggesting that fucoidan, but not laminaran, determined the anti-tumour activities. Therefore, polysaccharides from S. thunbergii might have great potential in anti-complement and anti-tumour application. Copyright © 2017 Elsevier B.V. All rights reserved.

Aplicación de la farmacoeconomía a los resultados de la medicación para la curación de las úlceras pépticas

Directory of Open Access Journals (Sweden)

Manuel M Collazo Herrera

2000-12-01

Full Text Available Se realizó una evaluación económica comparativa de los costos de los tratamientos quimioterapéuticos de 3 esquemas antiulcerosos (ranitidina, omeprazol y cimetidina; así como el análisis costo-efectividad para determinar la combinación de medicamentos que sería la más factible desde el punto de vista técnico-económico para la cicatrización de las úlceras pépticas y la erradicación del agente causante, el Helicobacter pylori. Como resultado se obtiene un esquema quimioterapéutico combinado para diferentes ciclos de tiempos en los tratamientos antiulcerosos (ranitidina-omeprazol, que incrementa la efectividad y disminuye los costos de los medicamentos para el sistema nacional de salud.A comparative economic evaluation of the costs of chemotherapeutic treatments of 3 anti-ulcer schemes (ranitidine, omeprazol and cimetidine, as well as the cost-effectiveness analysis were made to determine the most convenient drug combination for the cicatrization of peptic ulcer and for the erradication of its causal agent, Helicobacter pylori, from the economic and technical point of view. As a result, it was obtained a combined chemotherapeutic scheme for different cycles of time in the anti-ulcer treatments (ranitidine-omeprazol that increases the effectiveness and reduces the costs of drugs for the national health system.

[Anti-angiogenic drugs].

Science.gov (United States)

Sato, Yasufumi

2010-06-01

Angiogenesis or neovascularization, the formation of neo-vessels, is a physiological phenomenon endued in vasculature, but is involved in various pathological conditions. Angiogenesis is required for tumor growth and metastasis, and thus constitutes an important target for the control of tumor progression. Indeed, the recent development of bevacizumab, a neutralizing anti-VEGF monoclonal antibody as the first anti-angiogenic drug, legalized the clinical merit of anti-angiogenesis in cancers. Thereafter, various drugs targeting VEGF-mediated signals have been developed to control tumor angiogenesis. Thus, anti-angiogenic drugs are now recognized in the clinic as a major step forward for the treatment of cancers. This review focuses on the current status of antiangiogenesis treatment in cancers.

Measurement of b anti b correlations at the CERN p anti p collider

International Nuclear Information System (INIS)

Albajar, C.; Ankoviak, K.; Bartha, S.; Bezaguet, A.; Boehrer, A.; Bos, K.; Buchanan, C.; Buschbeck, B.; Castilla-Valdez, H.; Cennini, P.; Cittolin, S.; Clayton, E.; Cline, D.; Caughlan, J.A.; Dau, D.; Daum, C.; Della Negra, M.; Demoulin, M.; Denegri, D.; Dibon, H.; Dorenbosch, J.; Dowell, J.D.; Eggert, K.; Eisenhandler, E.; Ellis, N.; Evans, H.; Faissner, H.; Fensome, I.F.; Fortson, L.; Garvey, J.; Geiser, A. Givernaud, A.; Gonidec, A.; Gonzalez, B.; Gronberg, J.; Holthuizen, D.J.; Jank, W.; Jorat, G.; Kalmus, P.I.P.; Karimaeki, V.; Kenyon, I.; Kinnunen, R.; Krammer, M.; Lammel, S.; Landon, M.P.J.; Lemoigne, Y.; Levegruen, S.; Lipa, P.; Markou, C.; Markytan, M.; Maurin, G.; McMahon, S.; Merlo, J.P.; Meyer, T.; Moers, T.; Mohammadi, M.; Morsch, A.; Moulin, A.; Norton, A.; Otwinowski, S.; Pancheri, G.; Pietarinen, E.; Pimiae, M.; Placci, A.; Porte, J.P.; Priem, R.; Prosi, R.; Radermacher, E.; Rauschkolb, M.; Reithler, H.; Revol, J.P.; Robinson, D.; Rubbia, C.; Samyn, D.; Schinzel, D.; Schleichert, R.; Schroeder, M.; Seez, C.; Shah, T.P.; Sphicas, P.; Sumorok, K.; Szoncso, F.; Tan, C.H.; Taurok, A.; Taylor, L.; Tether, S.; Teykal, H.; Thompson, G.; Tuchscherer, H.; Tuominiemi, J.; Van de Guchte, W.; Van Dijk, A.; Vargas, M.; Virdee, T.S.; Schlippe, W. von; Vuillemin, V.; Wacker, K.; Wagner, H.; Walzel, G.; Wulz, C.E.; Zotto, P.; NIKHEF-H, Amsterdam; Birmingham Univ.; CERN, Geneva; Helsinki Univ.; Kiel Univ.; Blackett Lab., Imperial Coll., London; Queen Mary and Westfield Coll., London; MIT, Cambridge, MA; Rutherford Appleton Lab., Chilton; DPHPE/SEPh, CEN Saclay, Gif-sur-Yvette; California Univ., Los Angeles, CA; Oesterreichische Akademie der Wissenschaften, Vienna

1994-01-01

We report on measurements of correlated b anti b production in p anti p at √s=630 GeV, using dimuon data to tag both the b and anti b quarks. Starting from an inclusive dimuon sample we obtain improved cross-sections for single inclusive beauty production and confirm our earlier results on B 0 - anti B 0 mixing. From a study of b anti b correlations we derive explicit cross-sections for semi-differential b anti b production. We compare the measured cross-sections and correlations to OMIKRON (α s 3 ) QCD predictions and find good quantitative agreement. From the measured angular distributions we establish a sizeable contribution from higher order QCD processes with a significance of about seven standard deviations. A large nonperturbative contribution to these higher order corrections is excluded. (orig.)

ANTI-MICROBIAL AND ANTI-AMOEBIC ACTIVITY SOME AZOMETHINES - POTENTIAL TEXTILE DYESTUFFS

Directory of Open Access Journals (Sweden)

DJORDJEVIC Dragan

2016-05-01

Full Text Available In this paper, new synthesized three azomethine derivatives applied in dyeing textiles checking the anti-microbial properties of active components, at the same time [1-3]. The emphasis is thrown on the verification of anti-microbial properties that are important for obtaining textile with significantly improved performance. All compounds were characterized and evaluated for their anti-microbial activity against 7 pathogenic bacteria, 1 parasitic protozoan and 1 fungus. It estimated anti-bacterial activity in vitro against the following microorganisms Staphylococcus aureus, Bacillus anthracis, Streptococcus faecalis, Enterobacter sp., Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, and Candida albicans. The anti-amoebic activity in vitro was evaluated against the HM1: IMSS strain of Entamoeba histolytica and the results were compared with the standard drug, metronidazole. The synthesized azomethines, showed very good substantivity for wool fibers, gave fine coloring, with good degree of exhaustion after dyeing. The combination of extended synthetic analogues of natural molecules leads to discovery of chemical entities which might be excellent anti-microbial and anti-amoebic compounds as depicted in our results. Being highly the effects this compound can be explored in future as an option for decreasing pathogenic potential of infecting from different sources. Azomethines containing hydrazone (dyestuff 1 and phenylhydrazone (dyestuff 2 as moiety show average yield and moderate inhibition activity while azomethines containing thiosemicarbazone (dyestuff 3 as moiety show higher yield and greater inhibition activity towards gram-negative and gram-positive bacteria as well as a fungus.

Anti-carbamylated Protein Antibody Levels Correlate with Anti-Sa (Citrullinated Vimentin) Antibody Levels in Rheumatoid Arthritis.

Science.gov (United States)

Challener, Gregory J; Jones, Jonathan D; Pelzek, Adam J; Hamilton, B JoNell; Boire, Gilles; de Brum-Fernandes, Artur José; Masetto, Ariel; Carrier, Nathalie; Ménard, Henri A; Silverman, Gregg J; Rigby, William F C

2016-02-01

The presence of anticitrullinated protein antibodies (ACPA) in rheumatoid arthritis (RA) indicates a breach in immune tolerance. Recent studies indicate that this breach extends to homocitrullination of lysines with the formation of anti-carbamylated protein (anti-CarP) antibodies. We analyzed the clinical and serologic relationships of anti-CarP in 2 RA cohorts. Circulating levels of immunoglobulin G anti-CarP antibodies were determined by ELISA in established (Dartmouth-Hitchcock Medical Center) and early (Sherbrooke University Hospital Center) cohorts and evaluated for anticyclic citrullinated peptide antibodies (anti-CCP), specific ACPA, and rheumatoid factor (RF) levels using the Student t test and correlation analysis. We identified elevated anti-CarP antibodies titers in 47.0% of seropositive patients (Dartmouth, n = 164), with relationships to anti-CCP (p < 0.0001) and IgM-RF (p = 0.001). Similarly, 38.2% of seropositive patients from the Sherbrooke cohort (n = 171) had elevated anti-CarP antibodies; titers correlated to anti-CCP (p = 0.01) but not IgM-RF (p = 0.09). A strong correlation with anti-Sa was observed: 47.9% anti-Sa+ patients were anti-CarP antibodies+ versus only 25.4% anti-Sa- in the Sherbrooke cohort (p = 0.0002), and 62.6% anti-Sa+ patients versus 26.9% anti-Sa- were anti-CarP antibodies+ in Dartmouth (p < 0.0001). We found a more variable response for reactivity to citrullinated fibrinogen or to citrullinated peptides from fibrinogen and α enolase. In 2 North American RA cohorts, we observed a high prevalence of anti-CarP antibody positivity. We also describe a surprising and unexpected association of anti-CarP with anti-Sa antibodies that could not be explained by cross-reactivity. Further, considerable heterogeneity exists between anti-CarP reactivity and other citrullinated peptide reactivity, raising the question of how the pathogenesis of antibody responses for carbamylated proteins and citrullinated proteins may be linked in vivo.

Anti-oxidant and anti-inflammatory effects of rice bran and green tea ...

African Journals Online (AJOL)

Purpose: To investigate the anti-inflammatory and anti-oxidant properties of an enzyme bath of Oryza sativa (rice bran) and Camellia sinensis O. Kuntz (green tea) fermented with Bacillus subtilis (OCB). Methods: The anti-oxidant effects of OCB were assessed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay and flow ...

Serie: Madera para construcción Acabados para madera

OpenAIRE

Sáenz-Muñoz, Marta; Instituto Tecnológico de Costa Rica

2012-01-01

Durante mucho tiempo, la madera ha sido utilizada como material de construcción para resolver principalmente objetivos estructurales; sin embargo, actualmente, el uso de la madera ha tomado mucho auge en la estética de las construcciones, para brindar apariencia y calidez. En la actualidad, el mercado ofrece una amplia gama productos para dar diversos “acabados” a la madera, los cuales sirven para resaltar la belleza de algunas de ellas, realzar características como el color y la veta, o bien...

Hemolytic disease of the fetus and newborn caused by anti-E

Directory of Open Access Journals (Sweden)

Adiyyatu Sa′idu Usman

2013-01-01

Full Text Available Objective: Maternal allo-antibody production is stimulated when fetal red blood cells are positive for an antigen absent on the mother′s red cells. The maternal IgG antibodies produced will pass through the placenta and attack fetal red cells carrying the corresponding antigen. Allo-immune hemolytic disease of the fetus and newborn caused by anti-E rarely occurs. Case summary: We report two cases of anti-E hemolytic diseases in neonates. One of the neonates had severe hemolysis presenting with severe anemia, thrombocytopenia, and conjugated hyperbilirubinemia, while the other had moderate anemia and unconjugated hyperbilrubinemia. Although both the neonates were treated by phototherapy and intravenous immunoglobulin, one of them received double volume exchange transfusion. Conclusion: There appeared to be an increase in the occurrence of hemolytic disease of the fetus and newborn caused by Rh antibodies other than anti-D. In this case report, both patients presented with anemia and hyperbilirubinemia but were successfully treated, with a favorable outcome.

Estudo soroepidemiológico de leishmaniose em equinos na região de Araçatuba-SP, Brasil, área endêmica para leishmaniose visceral

Directory of Open Access Journals (Sweden)

Francisco Leydson Formiga Feitosa

2012-12-01

Full Text Available O presente estudo teve como objetivo pesquisar a presença de anticorpos anti-Leishmania sp. em equinos provenientes do município de Araçatuba-São Paulo, área endêmica para leishmaniose visceral canina e humana. Dos 466 equinos testados para a presença de IgG anti-Leishmania chagasi pelo método de ELISA, 68 (14,59% apresentaram sororeatividade, com títulos variando entre 0,324 e 0,813. As amostras de soro dos equinos positivos por ELISA foram testadas também por imunocromatografia, e 19/466 (4,08% apresentaram resultado positivo. Os resultados obtidos no presente estudo indicaram que esta espécie animal está em contato e deve atrair flebotomíneos transmissores da doença, e salientam a necessidade de investigação mais acurada sobre o real papel dos equinos residentes em áreas endêmicas, de forma a auxiliar nas medidas de controle da doença.

Absence of hemolytic disease of fetus and newborn despite maternal high-titer IgG anti-Ku.

Science.gov (United States)

Kakaiya, R M; Whaley, A; Howard-Menk, C; Rami, J; Papari, M; Campbell-Lee, S; Malecki, Z

2010-01-01

Anti-Ku seen in K(o) (Kell-null) individuals has previously been shown to cause severe hemolytic transfusion reactions. Maternal anti-Ku can cause none or moderate to severe hemolytic disease of the fetus and newborn (HDFN). In two of four previously described HDFN cases, intrauterine transfusions were required because of severe anemia. We report a case in which maternal anti-Ku did not cause HDFN. Standard serologic methods were used for RBC antibody screening and identification, adsorption and elution of RBC antibodies, and antigen typing. A gravida 3, para 3 (G3P3) woman was first evaluated in 2006 and was found to have an IgG RBC antibody that reacted against all panel RBCs in the anti-human globulin phase. A panel of RBCs treated with DTT did not react with the antibody. The antibody failed to react with one example of K(o) RBCs. The patient’s RBCs typed negative for the following Kell blood group antigens: KEL1, KEL2, KEL3, KEL4, KEL6, KEL7, KEL11, KEL13, and KEL18. These results established the presence of anti-Ku in maternal serum. The newborn was group A, D+ and required phototherapy for hyperbilirubinemia, but did not require transfusion. The woman was seen again in January 2010 during the third trimester (G4P3). At this time, anti-Ku titer was 256. She delivered a healthy group O, D+ baby boy at 37 weeks' gestation. Cord RBCs were 4+ for IgG by DAT. An eluate reacted with all RBCs tested, but did not react when tested against a panel of DTT-treated RBCs. K(o) phenotype is rare to begin with, and the maternal anti-Ku formation may require more than one pregnancy. Therefore, cases that can be evaluated for anti-Ku–related HDFN are rare. Our case contributes to serologic and clinical aspects of such rare cases.

Freqüência de alterações hepáticas em pacientes com esclerodermia Frequency of hepatic abnormalities in patients with scleroderma

Directory of Open Access Journals (Sweden)

Ariene Paixão

2007-10-01

Full Text Available OBJETIVO: avaliar a freqüência das doenças hepáticas em pacientes com esclerodermia e, secundariamente, estudar a freqüência de infecção pelos vírus B e C da hepatite nesses pacientes, assim como a freqüência de auto-anticorpos séricos. MATERIAL E MÉTODOS: estudaram-se pacientes com diagnóstico de esclerodermia, localizada ou sistêmica, acompanhados no Ambulatório de Reumatologia do Hospital Santa Izabel. Como grupo de comparação, foram estudados pacientes com diagnóstico de acne vulgar. RESULTADOS: dos 65 pacientes com diagnóstico de esclerodermia incluídos nesse trabalho, 35% apresentaram a gama-glutamiltransferase (gama-GT alterada, 30% tiveram a fosfatase alcalina aumentada e 17,1%, a alaninoaminotransferase (ALT acima dos valores de referência. A ALT apresentou-se mais alterada nos pacientes do que nos controles. Apenas um indivíduo dos 41 testados apresentou positividade para o anticorpo antimitocôndria enquanto 19% tinham anticorpo antimúsculo liso, não se observando diferença estatística na positividade desses anticorpos entre os dois grupos. Um paciente apresentou o HBsAg positivo e outro foi positivo para o anticorpo anti-HCV. Nenhum paciente apresentou manifestações clínicas de doença hepática. CONCLUSÕES: no presente estudo, embora as alterações de enzimas hepáticas em pacientes com esclerodermia não tenham sido incomuns, não se observou nenhum caso com manifestações clínicas de doença hepática.INTRODUCTION: to determine the frequency of hepatic disease in patients with scleroderma and, secondarily, to investigate the frequency of hepatitis B and C virus infection and determine the frequency of autoantibodies in this disease. MATERIAL AND METHODS: patients with scleroderma followed at Hospital Santa Izabel were included in the study and patients with acne vulgaris served as a comparison group. RESULTS: considering the 65 scleroderma patients, 35% had elevated gamma

Anti-nociceptive and anti-inflammatory properties of the ethanolic ...

African Journals Online (AJOL)

Anti-nociceptive and anti-inflammatory properties of the ethanolic extract of Lagenaria breviflora whole fruit in rat and mice. ... Its effect was comparable especially at 200mg/kg body weight to those of diclofenac, indomethacin and ibuprofen. It could be suggested from the findings of this experiment that the extract may be ...

Charmed meson rescattering in the reaction anti pd→ anti DDN

International Nuclear Information System (INIS)

Haidenbauer, J.; Krein, G.; Meissner, U.G.; Sibirtsev, A.

2008-01-01

We examine the possibility to extract information about the DN and anti DN interactions from the anti pd→D 0 D - p reaction. We utilize the notion that the open-charm mesons are first produced in the annihilation of the antiproton on one nucleon in the deuteron and subsequently rescatter on the other (the spectator) nucleon. The latter process is then exploited for investigating the DN and anti DN interactions. We study different methods for isolating the contributions from the D 0 p and D - p rescattering terms. (orig.)

The nontoxic natural compound Curcumin exerts anti-proliferative, anti-migratory, and anti-invasive properties against malignant gliomas

International Nuclear Information System (INIS)

Senft, Christian; Polacin, Margareth; Priester, Maike; Seifert, Volker; Kögel, Donat; Weissenberger, Jakob

2010-01-01

New drugs are constantly sought after to improve the survival of patients with malignant gliomas. The ideal substance would selectively target tumor cells without eliciting toxic side effects. Here, we report on the anti-proliferative, anti-migratory, and anti-invasive properties of the natural, nontoxic compound Curcumin observed in five human glioblastoma (GBM) cell lines in vitro. We used monolayer wound healing assays, modified Boyden chamber trans-well assays, and cell growth assays to quantify cell migration, invasion, and proliferation in the absence or presence of Curcumin at various concentrations. Levels of the transcription factor phospho-STAT3, a potential target of Curcumin, were determined by sandwich-ELISA. Subsequent effects on transcription of genes regulating the cell cycle were analyzed by quantitative real-time PCR. Effects on apoptosis were determined by caspase assays. Curcumin potently inhibited GBM cell proliferation as well as migration and invasion in all cell lines contingent on dose. Simultaneously, levels of the biologically active phospho-STAT3 were decreased and correlated with reduced transcription of the cell cycle regulating gene c-Myc and proliferation marking Ki-67, pointing to a potential mechanism by which Curcumin slows tumor growth. Curcumin is part of the diet of millions of people every day and is without known toxic side effects. Our data show that Curcumin bears anti-proliferative, anti-migratory, and anti-invasive properties against GBM cells in vitro. These results warrant further in vivo analyses and indicate a potential role of Curcumin in the treatment of malignant gliomas

Anti-microbial and anti-biofilm compounds from Indonesian medicinal plants

NARCIS (Netherlands)

Pratiwi, Sylvia U.T.

2015-01-01

Microbial biofilms causing elevated resistance to both most anti-microbial drugs and the host defense systems, which often results in persistent and difficult-to-treat infections. The discovery of anti-infective agents which are active against planktonic and biofilm microorganisms are therefore

Glutamic acid decarboxylase (anti-GAD & tissue transglutaminase (anti-TTG antibodies in patients with thyroid autoimmunity

Directory of Open Access Journals (Sweden)

R K Marwaha

2013-01-01

Full Text Available Background & objectives: Several autoimmune disorders have been reported to be associated with autoimmune thyroiditis and may coexist with other organ-specific autoantibodies. The aim of the present study was to evaluate the presence of tissue transglutaminase (anti-TTG and glutamic acid decarboxylase (anti-GAD antibodies in patients suffering from autoimmune thyroiditis as diagnosed by anti-thyroid peroxidase (anti-TPO antibodies, which may indicate high risk for developing celiac disease or type 1 diabetes mellitus. Methods: Five thousand children and 2800 adults were screening as part of a general health examination done on a voluntary basis in four different parts of Delhi. A total of 577 subjects positive for anti-TPO antibody constituted the cases. Equal number of age and sex matched anti-TPO antibody negative controls were randomly selected from the same cohort to form paired case control study. The cases and controls were further divided into two groups as follows: group-1 (children and adolescent 18 yr. Serum samples of cases and controls were analysed for thyroid function test (FT3, FT4, and TSH, anti-TTG and anti-GAD antibodies. Results: A total of 1154 subjects (577 cases and 577 controls were included in this study. Hypothyroidism was present in 40.2 per cent (232 cases compared to only 4.7 per cent (27 in controls (P<0.001. Anti-TTG and anti-GAD antibodies were present in 6.9 and 12.5 per cent subjects among cases compared to 3.5 per cent (P=0.015 and 4.3 per cent (P=0.001 in controls, respectively. Only anti-GAD antibody were significantly positive in cases among children and adolescents (P =0.0044 and adult (P=0.001 compared to controls. Levels of anti-TTG and anti-GAD antibodies increased with increasing titre of anti-TPO antibody. Interpretation & conclusions: Our findings showed high positivity of anti-GAD and anti-TTG antibodies among subjects with thyroid autoimmunity. It is, therefore, important to have high clinical index

Anti-nociceptive, anti-hyperalgesic and anti-arthritic activity of amides and extract obtained from Piper amalago in rodents.

Science.gov (United States)

da Silva Arrigo, Jucicléia; Balen, Eloise; Júnior, Ubirajara Lanza; da Silva Mota, Jonas; Iwamoto, Renan Donomae; Barison, Andersson; Sugizaki, Mario Mateus; Leite Kassuya, Cândida Aparecida

2016-02-17

Piper amalago (Piperaceae) has been used in folk medicine as an analgesic. This study aimed to evaluate the pharmacological effects of extract and pure amides obtained from P. amalago on pain to provide a pharmacological basis for their use in traditional medicine. This study evaluated the anti-nociceptive, anti-hyperalgesic, anti-arthritic and anti-depressive activities of the ethanolic extract of P. amalago (EEPA) and the amides N-[7-(3',4'-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl] pyrrolidine (1) and N-[7-(3',4'-methylenedioxyphenyl)-2(E),4(E)-heptadienoyl] pyrrolidine (2) obtained from P. amalago in animal models. Mice treated daily with EEPA (100mg/kg, p.o.) were assayed for 20 days for knee edema (micrometer measurement), mechanical hyperalgesia (analgesiometer analysis), heat sensitivity and immobility (forced swim test) in the Complete Freund's Adjuvant (CFA) model. Cold (acetone test) and mechanical hyperalgesia (electronic von Frey analysis) responses were evaluated for 15 days in rats treated with oral EEPA (100mg/kg) in the spared nerve injury (SNI) model. Meanwhile, mice were evaluated for carrageenan-induced edema and mechanical hyperalgesia and for nociception using the formalin model after a single administration of EEPA (100mg/kg) or amides 1 and 2 (1mg/kg). Amides (1) and (2) were detected and isolated from the EEPA. The EEPA inhibited mechanical hyperalgesia, knee edema, and heat hyperalgesia, but not depressive-like behavior, induced by the intraplantar injection of CFA. When evaluated in the SNI model, the EEPA inhibited mechanical and cold hyperalgesia. The EEPA, 1 and 2 prevented the mechanical hyperalgesia induced by carrageenan and the anti-nociceptive effects in both phases of formalin nociception. The EEPA did not induce alterations in the open field test. The EEPA was effective for inhibition of pain and arthritic parameters but was not effective against depressive-like behavior; additionally, it did not alter locomotor activity. The

Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica

Directory of Open Access Journals (Sweden)

Rosecler Riethmuller Franco

2011-02-01

Full Text Available FUNDAMENTO: A síndrome metabólica está associada ao aumento de risco de eventos cardiovasculares. Marcadores inflamatórios e anticorpos anti-Chlamydia têm sido relacionados ao desenvolvimento e à progressão da aterosclerose e dos eventos cardiovasculares. OBJETIVO: Avaliar os marcadores inflamatórios interleucina-6 (IL-6 e fator de necrose tumoral-alfa (TNF-α e os anticorpos anti-Chlamydia pneumoniae em pacientes com síndrome metabólica (SM, com e sem eventos cardiovasculares. MÉTODOS: Estudo transversal constituído por 147 indivíduos. Desses, 100 (68% com SM e sem eventos cardiovasculares; e 47 (32% com SM e com eventos cardiovasculares. Dos indivíduos que sofreram eventos cardiovasculares, 13 (6,11% apresentam infarto agudo do miocárdio (IAM, e dez (4,7%, acidente vascular cerebral (AVC. O diagnóstico da SM foi determinado pelos critérios do NCEP-ATPIII. RESULTADOS: A média de idade dos sujeitos com eventos cardiovasculares foi de 61,26 ± 8,5 e de 59,32 ± 9,9 nos indivíduos sem esses eventos (p=0,279, havendo predomínio do sexo feminino. O grupo com SM e sem evento apresentou maior peso, altura, IMC e circunferência abdominal. Para os indivíduos com eventos cardiovasculares (p=0,001, os marcadores inflamatórios IL-6 e TNF-α e a doença vascular periférica foram significativamente maiores. Obtiveram-se níveis elevados de anticorpos IgG para Chlamydia pneumoniae no grupo SM, sem eventos e de IgA no grupo com eventos quando comparados os dois grupos. Com relação ao IAM e ao AVC, os anticorpos anti-Chlamydia pneumoniae não demonstraram significância estatística, comparados ao grupo sem eventos cardiovasculares. Associação foi observada com o uso de estatinas, hipoglicemiantes orais, injetáveis e anti-inflamatórios não esteroidais no grupo com esses eventos. CONCLUSÃO: Marcadores inflamatórios encontram-se significativamente elevados em pacientes com SM, com IAM e AVC. Anticorpos anti-Chlamydia n

Anti-Saccharomyces cerevisiae and perinuclear anti-neutrophil cytoplasmic antibodies in coeliac disease before and after gluten-free diet.

Science.gov (United States)

Granito, A; Zauli, D; Muratori, P; Muratori, L; Grassi, A; Bortolotti, R; Petrolini, N; Veronesi, L; Gionchetti, P; Bianchi, F B; Volta, U

2005-04-01

Anti-Saccharomyces cerevisiae and perinuclear anti-neutrophil cytoplasmic autoantibodies are markers of Crohn's disease and ulcerative colitis respectively. To determine the prevalence of anti-S. cerevisiae and perinuclear anti-neutrophil cytoplasmic autoantibodies in a large series of coeliac disease patients before and after gluten free diet, and to correlate anti-S. cerevisiae-positivity with intestinal mucosal damage. One hundred and five consecutive coeliac disease patients and 141 controls (22 ulcerative colitis, 24 Crohn's disease, 30 primary sclerosing cholangitis, 15 postenteritis syndrome, 50 blood donors) were tested for anti-S. cerevisiae by enzyme-linked immunosorbent assay and for perinuclear anti-neutrophil cytoplasmic autoantibodies by indirect immunofluorescence. In coeliac disease anti-S. cerevisiae (immunoglobulin G and/or immunoglobulin A) were slightly less frequent (59%) than in Crohn's disease (75%, P = 0.16) and significantly more frequent than in ulcerative colitis (27%), primary sclerosing cholangitis (30%), postenteritis syndrome (26%) and blood donors (4%) (P = 0.009, P = 0.0002, P = 0.025, P < 0.0001). No correlation was found between anti-S. cerevisiae and degree of mucosal damage. Perinuclear anti-neutrophil cytoplasmic autoantibodies were detected only in one coeliac. After gluten free diet the disappearance of anti-S. cerevisiae-immunoglobulin A (93%) was more frequent than that of immunoglobulin G (17%, P = 0.0001); perinuclear anti-neutrophil cytoplasmic autoantibodies disappeared in the only coeliac positive at diagnosis. More than half of untreated coeliacs are anti-S. cerevisiae-positive irrespective of the severity of mucosal damage. Differently from immunoglobulin A, anti-S. cerevisiae-immunoglobulin G persisted in more than 80% after gluten free diet. The high prevalence of anti-S. cerevisiae in coeliac disease suggests that they may be the effect of a non-specific immune response in course of chronic small bowel disease.

Antioxidant and anti-inflammatory properties of 1,2,4-oxadiazole analogs of resveratrol.

Science.gov (United States)

Gobec, Martina; Tomašič, Tihomir; Markovič, Tijana; Mlinarič-Raščan, Irena; Dolenc, Marija Sollner; Jakopin, Žiga

2015-10-05

The chemopreventive properties of resveratrol are ascribed mostly to its antioxidant activity, in particular its scavenging ability for reactive oxygen species (ROS), and to the inhibition of NF-κB pathway which has also been suggested as an important underlying mechanism of its reported properties. In present study, a small library of nine 1,2,4-oxadiazole-based structural analogs of resveratrol were assayed for their antioxidant and anti-inflammatory activities. Several compounds showed significant inhibitory activities against NF-κB and/or ROS production. Compound 2, incorporating two para-hydroxyphenyl moieties connected by the 1,2,4-oxadiazole ring, was the most active, its potency in inhibiting activation of NF-κB and ROS scavenging abilities surpassing that of resveratrol. Additionally, we elucidated the mechanisms underlying the NF-κB inhibitory activity of compound 2. Finally, in contrast to resveratrol, compound 2 significantly reduced the LPS-induced release of pro-inflammatory cytokines, indicating its prominent anti-inflammatory potential. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Evidências científicas para a indicação popular de algumas espécies da família Rutaceae no tratamento de doenças respiratórias na região Sudeste do Brasil

Directory of Open Access Journals (Sweden)

Renata Correia Campello PRUDENTE, Regina Braga de MOURA

2013-12-01

Full Text Available A utilização de plantas medicinais como forma de tratamento vem sendo bastante empregada pela população brasileira, sendo a maioria através do uso empírico. Diversas patologias têm sido tratadas através da utilização popular de plantas medicinais, sendo uma das principais as doenças do trato respiratório. O objetivo do presente trabalho foi verificar se existem evidências científicas para o uso popular das espécies de Rutaceae: Citrusaurantiifolia (Christm. Swingle, Citrus aurantiumL., Citrus limonum Risso, Citrus medica L., Citrussinensis Osbeck e Ruta graveolens L. na regiãoSudeste, para problemas respiratórios. Foram considerados artigos sobre estudos farmacológicos envolvendo doenças respiratórias, assim como sobre a ação anti-inflamatória e anti-bacteriana das espécies. A revisão mostrou que a presença de flavonóides, hidrocarbonetos terpênicos e monoterpênicos nos extratos e nos óleos essenciais dessas plantas causam um efeito anti-inflamatório, através da inibição de mediadores pró-inflamatórios iNOS e COX-2; e atividade anti-bacteriana, analisada por meio do método de difusão em disco. O trabalho mostrou que apesar do intenso uso da população da região Sudeste do Brasil de espécies medicinais da família Rutaceae para tratamento de doenças do trato respiratório, ainda existem poucos estudos que evidenciem cientificamente as ações farmacológicas, justificando assim a grande necessidade de estudos clínicos para tratamentos feitos com a utilização das espécies estudadas.

Single phase semipolar (11 anti 22) GaN on (10 anti 10) sapphire

Energy Technology Data Exchange (ETDEWEB)

Ploch, S.; Stellmach, J.; Schwaner, T.; Frentrup, M.; Wernicke, T.; Pristovsek, M.; Kneissl, M. [Institute of Solid States Physics, (Germany); Park, J.B.; Niermann, T.; Lehmann, M. [Institute of Optics and Atomic Physics, TU Berlin, Hardenbergstr. 36, 10623 Berlin (Germany)

2011-07-01

InGaN quantum well based light emitters grown on (0001) GaN suffer from poor quantum efficiencies with increasing indium mole fraction due to strong polarization fields along the polar crystal orientation. This effect can be greatly reduced by growing on semi- and non-polar GaN orientations. Semipolar (11 anti 22) GaN layers were deposited by metalorganic vapour phase epitaxy on (10 anti 10) sapphire. After sapphire substrate nitridation at 1000 C, a GaN nucleation layer was deposited at high temperature, followed by the deposition of 1.5 nm thick GaN buffer layers. The samples show predominantly (11 anti 22) orientation with a small fraction of (10 anti 13) oriented domains. With increasing nitridation layer thickness the (10 anti 13) phase is suppressed leading to a very smooth surface morphology (rms roughness < 4nm). PL measurements show dominant basel plane stacking fault (BSF) I{sub 1} luminescence without any other defects. Transmission electron microscopy measurements reveal a high BSF density. The FWHM of the X-ray diffraction rocking curve measurements of the (1122) reflection decreases to 1193 arcsec and 739 arcsec along [1 anti 100] and [11 anti 23] respectively with increasing nucleation temperature. Using high temperature nucleation smooth and homogeneous (11 anti 22) phase GaN layers have been obtained.

Production of light (anti-)nuclei and (anti-)hypernuclei with ALICE at the LHC

CERN Multimedia

CERN. Geneva

2017-01-01

The measurements in smaller collision systems may also provide an input to cosmological searches for segregated primordial anti-matter and dark matter, since anti-nuclei produced in pp and p--A collisions in interstellar space represent a background source in these measurements. Thanks to its excellent particle identification and tracking capabilities, the ALICE detector allows for the measurement of deuterons, tritons, 3He, 4He and their corresponding anti-nuclei. Moreover, the secondary vertices from the mesonic decays of (anti-)hypernuclei can ...

The anti-aging and anti-oxidation effects of tea water extract in Caenorhabditis elegans.

Science.gov (United States)

Fei, Tianyi; Fei, Jian; Huang, Fang; Xie, Tianpei; Xu, Jifeng; Zhou, Yi; Yang, Ping

2017-10-15

Tea includes puer tea, black tea, green tea and many others. By using model organism Caenorhabditis elegans, the anti-aging and anti-oxidation effects of tea water extract were systemically examined in this study. We found that water extract of puer tea, black tea and green tea all increased the lifespan of worms, postponed Aβ-induced progressive paralysis in Alzheimer's disease transgenic worms, and improved the tolerance of worms to the oxidative stress induced by heavy metal Cr 6+ . Moreover, the anti-oxidation effects of tea water extract at low concentration were different among 4 kinds of brands of green tea. The underlying mechanisms were further explored using genetically manipulated-mutant worms. The anti-oxidative stress effects of green tea water extract depend on the dietary restriction and germline signaling pathways, but not the FOXO and mitochondrial respiratory chain signals. Therefore, tea water extract provides benefits of anti-aging, anti-AD and anti-oxidation. Copyright © 2017. Published by Elsevier Inc.

Anti-beta2 glycoprotein 1 and the anti-phospholipid syndrome.

LENUS (Irish Health Repository)

Keane, Pearse A

2012-02-03

PURPOSE: To describe a patient who presented with bilateral retinal vascular occlusion and the use of anti-beta2 glycoprotein 1 (GPI) antibody testing in the diagnosis of antiphospholipid syndrome. DESIGN: Observational case report. METHODS: Hematological investigations were performed on a 49-year-old man who presented with rapid onset of bilateral severe central retinal vein occlusion. RESULTS: Lupus anticoagulant and anticardiolipin antibody testing was negative. Markedly raised titers of anti-beta2 GPI antibodies were detected on two separate occasions. CONCLUSIONS: The raised titers of anti-beta2 GPI antibodies were considered to strongly suggest an underlying diagnosis of the antiphospholipid syndrome.

Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance Caracterização de uma cepa de hepatite por vírus B no sudoeste do Paraná, Brasil, apresentando mutações previamente associadas à resistência anti-HBs

Directory of Open Access Journals (Sweden)

Dennis Armando Bertolini

2010-02-01

Full Text Available The present study investigated if hepatitis B virus (HBV mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.O presente estudo investigou se mutantes do vírus da hepatite B (HBV circulam na região Sudoeste do Estado do Paraná, Brasil, analisando amostras de crianças que receberam a imunoprofilaxia por terem nascido de mães portadoras do HBV. Amostras de 25 crianças foram analisadas para os marcadores sorológicos do HBV e para o DNA-HBV por PCR. Somente uma amostra foi positiva para AgHBs, anti-HBs e DNA-HBV, apesar da criança ter sido vacinada. Análises da seqüência do gene S desta amostra mostrou a presença de uma prolina na posição 105, uma serina na posição 114, três treoninas nas posições 115, 116 e 140, e uma glutamina na posição 129. A presen

A importância da contra-imunoeletroforese na detecção de antígenos nucleares extraíveis para o diagnóstico de doenças reumáticas sistêmicas The importance of counterimmunoelectrophoresis in the detection of extractable nuclear antigens for the diagnosis of systemic rheumatic diseases

Directory of Open Access Journals (Sweden)

Rita de Cassia Siqueira Bruder

2004-02-01

Full Text Available Os antígenos nucleares extraíveis (ENAs são encontrados no soro da maioria dos pacientes com doença reumática sistêmica. Os principais ENAs estudados são SS-A/Ro, SS-B/La, RNP, Sm, Scl-70 e Jo-1. Objeti-vou-se neste trabalho: a padronizar a técnica de contra-imunoeletroforese (CIE para a detecção de ENAs; b padronizar o substrato antigênico (ENAs para a CIE a partir de baço de cão; c comparar os resultados da CIE com as técnicas de imunofluorescência indireta (IFI e Elisa para esses antígenos. Para tal foram estudados 40 soros de pacientes com doença reumática sistêmica confirmada por exames clínico e laboratorial (sorológico e biópsia. Como controle negativo foram utilizados dez soros de doadores de sangue normais, e, como controles positivos, seis soros-padrão anti-Ro, anti-La, anti-RNP, anti-Sm, anti-Scl-70, anti-Jo-1, para caracterizar os antígenos presentes no extrato de baço. Neste último foram detectados vários ENAs, exceto RNP e Scl-70. A técnica de CIE apresentou boas sensibilidade (70% e especificidade (100% em relação às outras técnicas (IFI e Elisa. A titulação dos soros pela CIE revelou positividade até diluições de 1:16 em 32,5% dos casos. Concluímos que a CIE e os antígenos extraídos de baço de cão podem ser utilizados na rotina laboratorial para triagem destes ENAs, com a vantagem, em relação à IFI, de poderem ser titulados.Extractable nuclear antigens (ENAs are detected in the sera of the majority of patients with rheumatic systemic disease. The main ENAs studied are SS-A/Ro, SS-B/La, RNP, Sm, Scl-70 and Jo-1. The aim of this work was: a to standardize the counterimmunoelectrophoresis technique (CIE to detect ENAs; b to standardize the extration of ENAs from dog spleen as a substract for CIE test; c to compare the results obtained by CIE with those of indirect immunofluorescence (IFI and Elisa for these antigens. Forty sera from individuals with rheumatic systemic disease confirmed by

Prevalência de anticorpos para o vírus da varicela-zoster em adultos jovens de diferentes regiões climáticas brasileiras

Directory of Open Access Journals (Sweden)

Reis Alexanda Dias

2003-01-01

Full Text Available Para avaliar a prevalência da infecção pelo vírus da varicela-zoster, de regiões urbanas de diferentes regiões do Brasil, 975 amostras de soro provenientes de adultos jovens doadores de sangue com idade entre 20 e 29 anos, de cidades de clima tropical (Salvador e Fortaleza e de clima temperado (São Paulo, Curitiba e Porto Alegre foram processadas pelo teste imunoenzimático doméstico para pesquisa de anticorpos IgG anti-Vírus da varicela zoster. A soroprevalência global de anticorpos anti-virus da varicela zoster nas várias regiões estudadas foi de 94,2%. A menor taxa (88,7% foi observada em Fortaleza e a maior em Curitiba (99,5%. A soroprevalência nas regiões de clima tropical (89,4% foi significativamente inferior a soroprevalência nas regiões de clima temperado (97,3%, seguindo um padrão similar à infecção em outros países de clima tropical.

The Salivary Anti-A and Anti-B Isoantibody System in Group O Males.

Science.gov (United States)

agglutinins increased significantly in two individuals and anti-B in four, and parotid saliva anti-A agglutinins in one and anti-B in three. Both whole...and parotid saliva agglutinating activity were demonstrated to be primarily due to secretory IgA, both pre- and postimmunization. IgG coating activity

Desempenho de um filme livre de cromo na proteção contra a corrosão de ligas de alumínio 2024.

OpenAIRE

Mayra Rúbia Silva Castro

2001-01-01

Este trabalho propõe o uso de um filme preparado a partir de solução aquosa alcalina contendo íons de lítio, borato e alumínio, no sentido de inibir a corrosão de ligas de alumínio de uso aeronáutico, buscando sempre comparações com filmes de sais de cromo, atualmente utilizados. Os filmes foram obtidos por dip coating (5 minutos) e por polarização anódica (5 e 45 minutos) de ligas de alumínio 2024-T3 na solução aquosa alcalina contendo íons de lítio, borato e alumínio. Foram feitas micrograf...

Anti-synthetase syndrome associated with anti PL-12 and anti-Signal recognition particle antibodies and a necrotizing auto-immune myositis.

Science.gov (United States)

Malkan, Ashish; Cappelen-Smith, Cecilia; Beran, Roy; Griffith, Neil; Toong, Catherine; Wang, Min-Xia; Cordato, Dennis

2015-02-01

We report a 37-year-old woman with a 2 month history of proximal muscle weakness and extremely high creatine kinase (21,808 U/L) due to necrotizing auto-immune myositis (NAM) in association with anti-synthetase syndrome. Myositis-specific auto-immune antibody panel was positive for anti-Signal recognition particle and anti-PL-12. CT scan of the chest confirmed interstitial lung disease. Prednisolone, intravenous immunoglobulin and cyclophosphamide therapy was given with gradual improvement. This patient is notable for the unusual combination of NAM and anti-synthetase syndrome with the rare finding of two myositis-specific autoantibodies, which directed testing for associated extramuscular features and management with more aggressive immunotherapy. Copyright © 2014 Elsevier Ltd. All rights reserved.

Synthesis, structure analysis, anti-bacterial and in vitro anti-cancer ...

Indian Academy of Sciences (India)

DOI 10.1007/s12039-015-0824-z. Synthesis, structure analysis, anti-bacterial and in vitro anti-cancer activity of new Schiff base and its copper complex derived from sulfamethoxazole. I RAMA∗ and R SELVAMEENA. PG and Research Department of Chemistry, Seethalakshmi Ramaswami College,. Tiruchirappalli 620 002 ...

Para-bosons and Para-fermions in Quantum Mechanics

International Nuclear Information System (INIS)

Cattani, M.S.D.; Fernandes, N.C.

1982-01-01

Within the framework of the ordinary quantum mechanics, a detailed study of the energy eigenfunctions of N identical particles using the irreducible representations of the permutation group in the Hilbert space is performed. It is shown that the para-states, as occurs with the boson and fermion states, are compatible with the postulates of quantum mechanics and with the principle of indistinguishability. A mathematical support for the existence of para-bosons and para-fermions is given. Gentile's quantum statistics is, in a certain sense, justified. (Author) [pt

Anti-inflammatory activity of Adenanthera pavonina L., Fabaceae, in experimental animals Atividade anti-inflamatória de Adenanthera pavonina L., Fabaceae, em animais experimentais

Directory of Open Access Journals (Sweden)

Arzumand Ara

2010-12-01

Full Text Available Adenanthera pavonina L, Fabaceae alt. Leguminosae, Bengali name 'rakta kombol', is an Indian medicinal plant. It is endemic to Southern China and India, and widely naturalized in Malaysia, Western and Eastern Africa as well as in most islands of both the Pacific and Caribbean regions. This plant has been used in traditional medicine for the treatment of asthma, boil, diarrhoea, gout, inflammations, rheumatism, tumour and ulcers, and as a tonic. The dried and ground bark of A. pavonina L. was extracted, successively, with petroleum ether (PE, dichloromethane (DCM, ethyl acetate (EtOAc and methanol (MeOH, and the resulting extracts were assessed in vivo for anti-inflammatory activity on carrageenan-induced rat hind paw oedema as a model of inflammation. The extracts were administered orally at the doses of 200 and 400 mg/kg body weight, and statistically significant (pAdenanthera pavonina L, Fabaceae, conhecida na região de Bengala por "rakta kombol", é uma planta medicinal endêmica do sul da China e da Índia e amplamente difundida na Malásia, África Ocidental e Oriental, bem como na maioria das ilhas de ambas as regiões do Pacífico e Caribe. Esta planta tem sido utilizada na medicina tradicional para o tratamento da asma, febre, diarréia, gota, inflamações, reumatismo tumor, úlceras, e como tônico. Cascas secas e moídas de A. pavonina foi submetida a extração sucessiva com éter de petróleo (PE, diclorometano (DCM, acetato de etila (AcOEt e metanol (MeOH, e os extratos foram avaliados in vivo para atividade anti-inflamatória induzida por carragenina tendo como ensaio modelo de inflamação edema de pata em ratos.. Os extratos foram administrados por via oral em doses de 200 e 400 mg/kg de peso corporal, e foram observadas efeitos anti-inflamatórios de maneira dose-dependente, estatisticamente significativos (p<0,001. O extrato metanólico (400 mg/kg apresentou 37,10% (p<0,01 de inibição da inflamação na primeira hora do

Dietary fish oil modulates the effect of dimethylhydrazine- induced colon cancer in rats

Directory of Open Access Journals (Sweden)

Rasmy, G. E.

2011-09-01

suplementación de aceite de pescado en la carcinogénesis de colon en ratas wistar machos. Para la inducción del cáncer de colon, las ratas fueron tratadas semanalmente con una inyección subcutánea de 1,2-dimethyl hydrazine (DMH a una dosis de 20 mg/kg de peso durante cinco semana. A continuación, algunas ratas tomaron aceite de pescado durante 4 semanas, o durante 17 semanas (grupo DMH-FO17. Las ratas restantes continuaron sin ningún tipo de suplementación durante las mismas 4 semanas (grupo DMH4, o 17 semanas (grupo DMH17. Otros dos grupos de ratas no recibieron DMH y un grupo consumió aceite de pescado (grupo FO17 y el otro solamente una dieta normal y es considerado como el grupo control (grupo CN. Al final del experimento, las ratas fueron sacrificadas; y a continuación fueron evaluadas mediante análisis bioquímico y biología molecular así como examen histopatológico. El resultado mostro un incremento en los niveles de lactato deshidrogenasa (LDH, malondialdehido (MDS y fosfatasa alcalina (ALP en ratas DMH en comparación con el control. Los cambios inducidos en el hígado y el colon por DMH fueron significativamente mejorados con la suplementación de aceite de pescado en el grupo DMH-FO17. El análisis molecular revelo que el tratamiento con DMH indujo alteraciones de la expresión de los genes p53, p27 y p21 e incremento el perfil de bandas de DNA relacionadas con el cáncer, mientras que los grupos FO17 Y DMH-FO17 mostraron mejores resultados. El examen histológico del grupo DMH17 revelo adenocarcinoma de colon y algunas lesiones en tejidos de hígado de rata. La mejora en el perfil histológico fue notable en el hígado y colon del grupo DMH-FO17. En conclusión, los presente resultados demuestran el efecto anti-carcinogénico del aceite de arenque contra la carcinogénesis de colon inducida por DMH en ratas. El efecto inhibidor de FO fue debido a la modulación de parámetros bioquímicos elevados, daño a DNA y lesiones histopatológicas causadas

Anti-Halal and Anti-Animal Slaughtering Campaigns and Their Impact in Post-War Sri Lanka

Directory of Open Access Journals (Sweden)

Mohammad Agus Yusoff

2017-03-01

Full Text Available This paper aims to examine the overall impact of anti-halal and anti-slaughtering campaigns in the context of post-war Sri Lanka. The reemergence of majoritarian ethno-religious anti-minority nationalist forces and their intensified anti-minority hatred and violence have made it challenging for ethno-religious minorities in Sri Lanka to engage in religious norms and duties. This is especially true for the Muslim community. Numerous Islamic fundamentals have been criticized and opposed. Muslims have had to endure threats and acts of violence. These campaigns and violent oppositions, imposed by the Buddhist-nationalist forces, have caused concern for Muslims performing their obligatory religious duties and norms. In Sri Lanka, the Muslim community has been allowed to produce halal food and slaughter animals for human consumption and religious rituals for a long period without disturbance. Unfortunately, retaliation and hatred in the post-civil war era in the country have threatened these rights. Thus, it has become imperative to investigate the motivating factors of the anti-halal and anti-animal slaughtering campaigns and violence, as well as their related impact, which is lacking in the existing literature on ethno-religious politics in the context of Sri Lanka. This study found that the anti-halal and anti-animal slaughtering campaigns and oppositions that have been intensified by the Buddhist nationalist forces were part of anti-Muslim sentiments intended to sabotage the economic pride of Muslims and undermine their religious renaissance. The study also found that these campaigns have been facilitated by the state and that continuous facilitation of the anti-Muslim sentiments and campaigns, including the anti-halal and anti-animal slaughter campaigns, would challenge the country’s economic prosperity and the rebuilding of ethno-religious harmony.

Prevalência de anticorpos para chlamydia trachomatis em grupos populacionais do Brasil, Inglaterra e Portugal

Directory of Open Access Journals (Sweden)

Marluísa de Oliveira Guimarães Ishak

1988-02-01

Full Text Available A prevalência de anticorpos IgG, grupo-específico para Chlamydia, em populações do Brasil, Inglaterra e Portugal foi determinada através do teste de imunofluorescência indireta, tendo-se como antígeno a cepa SA2 (f. Foram considerados positivos os soros com títulos de IgG >1:32. Dentre as populações brasileiras, a prevalência de anticorpos para Chlamydia foi maior em Serra Norte (76,2%, p < 0,01 do que nas das populações de Belém (53,6% e dos Índios Xicrins (51,3%. Entre os pacientes do Departamento de Medicina Genito-Urinária do University College Hospital (UCH e do quadro do mesmo Hospital, a prevalência de anticorpos anti-Chlamydia foi de 62% e 53,1%, respectivamente. Anticorpos anti-Chlamydia foram detectados em 54% e 66% na Inglaterra e em 56% e 68% em Portugal, nas pacientes do sexo feminino que freqüentavam Clínicas de Pré-Natal e de Infertilidade, respectivamente, Os resultados encontrados mostram uma alta exposição das populações testadas, à Chlamydia, principalmente do grupo de baixo nível sócio-econômico de Serra Norte, Brasil. A evidência de infecção por Chlamydia é da mesma ordem, tanto no Brasil, quanto na Inglaterra e Portugal.

Prevalencia de anticuerpos anti-Chlamydia trachomatis y anti-Neisseria gonorrhoeae en grupos de individuos de la población mexicana Prevalence of antibodies against Chlamydia trachomatis and Neisseria gonorrhoeae in Mexican populations

Directory of Open Access Journals (Sweden)

María del Carmen Cravioto

2003-01-01

Full Text Available OBJETIVO: Estimar la prevalencia de infección por Chlamydia trachomatis (CT y Neisseria gonorrhoeae (NG en grupos de individuos con diferente riesgo para infecciones de transmisión sexual. MATERIAL Y MÉTODOS: Estudio transversal multicéntrico efectuado en el Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, de la Ciudad de México, de enero de 1992 a diciembre de 1993, en el que se estudiaron 945 individuos en edad reproductiva, 585 mujeres y 360 hombres. Según su riesgo para infecciones de transmisión sexual se clasificaron en grupos de alto y bajo riesgo. Los de alto riesgo incluyeron mujeres infértiles con daño tubario, mujeres con embarazo ectópico o aborto, hombres infértiles, pacientes con VIH/SIDA, hombres homo o bisexuales y trabajadoras del sexo comercial (TSC. Los de bajo riesgo: mujeres primigestas, hombres fértiles y mujeres infértiles sin daño tubario. Se determinó en duplicado IgG e IgA anti-NG y anti-CT en suero, por análisis inmunoenzimático, utilizando como antígeno el pili de NG y la fracción L1 de CT. Se calcularon porcentajes. RESULTADOS: En mujeres la prevalencia para NG fue IgG 13.7% e IgA 14.3%, y para CT fue IgG 11.4% e IgA 4.4%. En hombres, NG 3.3% y 13.3%, respectivamente, y para CT 7.2% y 5.5%, respectivamente. En TSC se encontró NG en 31.2 % y 28.4%, respectivamente, y para CT 25.0% y 5.7%, respectivamente. En mujeres con infertilidad por daño tubario, NG 5.6% y 9.8%, respectivamente, y CT 8.4% y 1.4%, respectivamente. En 110 primigestas jóvenes, NG 4.5% y 10.0%, respectivamente, y CT 3.6% y 9.1%, respectivamente. CONCLUSIONES: Estos datos confirman la prevalencia elevada de Neisseria gonorrhoeae y Chlamydia trachomatis en trabajadoras del sexo comercial y en hombres homo/bisexuales, pero no en otros grupos de alto riesgo como las mujeres infértiles, con aborto o embarazo ectópico.OBJECTIVE: To estimate the prevalence of Chlamydia trachomatis (CT and Neisseria gonorrhoeae

Experimental determination of the complete spin structure for anti-proton + proton -> anti-\\Lambda + \\Lambda at anti-proton beam momentum of 1.637 GeV/c

CERN Document Server

Paschke, K.D.; Berdoz, A.; Franklin, G.B.; Khaustov, P.; Meyer, C.A.; Bradtke, C.; Gehring, R.; Goertz, S.; Harmsen, J.; Meier, A.; Meyer, W.; Radtke, E.; Reicherz, G.; Dutz, H.; Pluckthun, M.; Schoch, B.; Dennert, H.; Eyrich, W.; Hauffe, J.; Metzger, A.; Moosburger, M.; Stinzing, F.; Wirth, St.; Fischer, H.; Franz, J.; Heinsius, F.H.; Kriegler, E.; Schmitt, H.; Bunker, B.; Hertzog, D.; Jones, T.; Tayloe, R.; Broders, R.; Geyer, R.; Kilian, K.; Oelert, W.; Rohrich, K.; Sachs, K.; Sefzick, T.; Bassalleck, B.; Eilerts, S.; Fields, D.E.; Kingsberry, P.; Lowe, J.; Stotzer, R.; Johansson, T.; Pomp, S.; Wirth, St.

2006-01-01

The reaction anti-proton + proton -> anti-\\Lambda + \\Lambda -> anti-proton + \\pi^+ + proton + \\pi^- has been measured with high statistics at anti-proton beam momentum of 1.637 GeV/c. The use of a transversely-polarized frozen-spin target combined with the self-analyzing property of \\Lambda/anti-\\Lambda decay allows access to unprecedented information on the spin structure of the interaction. The most general spin-scattering matrix can be written in terms of eleven real parameters for each bin of scattering angle, each of these parameters is determined with reasonable precision. From these results all conceivable spin-correlations are determined with inherent self-consistency. Good agreement is found with the few previously existing measurements of spin observables in anti-proton + proton -> anti-\\Lambda + \\Lambda near this energy. Existing theoretical models do not give good predictions for those spin-observables that had not been previously measured.

Lutar pelo quê? Reflexões sobre os rumos dos movimentos antisistêmicos Fight for what? Thoughts about the anti-systemic activity trends

Directory of Open Access Journals (Sweden)

Giane Carmem Alves de Carvalho

2008-12-01

Full Text Available Os chamados movimentos anti-sistêmicos vêm, segundo Wallerstein, intensificando-se cada vez mais nos tempos atuais. As contradições, conflitos e tensões, frutos da hegemonia do capital, permitiram uma ressignificação dos movimentos sociais, visando articular novas lutas numa perspectiva mundial, ou seja, a globalização do capital possibilitou a globalização dos movimentos anti-sistêmicos. No momento em que os movimentos anti-sistêmicos são vistos como principal referência para a incessante luta contra o capital, questiona-se aqui, afinal, o que vem a ser um movimento anti-sistêmico. Apesar das evidências de seu surgimento, em que sentido é possível afirmar sua legitimidade? Nesta luta quem é o verdadeiro inimigo? Desta forma, tem-se como objetivo discutir, sob uma abordagem crítica e reflexiva, uma breve evolução histórica dos movimentos anti-sistêmicos, as linhas conceituais, os rumos e impasses político-ideológicos dos chamados movimentos anti-sistêmicos. Palavras-chave: Movimentos anti-sistêmicos. Movimentos sociais. Globalização. The anti-systemic activities are, according to Wallerstein, getting enhanced more and more, nowadays. The contradictions, conflicts and tensions, consequences of capitalism, have opened the way for new significances inside social activities intending to articulate new fights in a world perspective, in other words, the capital globalization has allowed the globalization of anti-systemic activities. At the time the anti-systemic activities are seen as main reference to the never-ending fight against capital, we question, after all, what really is an anti-systemic activity. Despite the evidences of its beginning, how can we affirm its legitimacy? Who is the real enemy in this fight? This way, we have, as a goal, a critical and reflexive discussion, a brief historical evolution of the anti-systemic activities: the conceptual lines, the courses and the political and ideological

Four anti-protozoal and anti-bacterial compounds from Tapirira guianensis.

Science.gov (United States)

Roumy, Vincent; Fabre, Nicolas; Portet, Bénédicte; Bourdy, Geneviève; Acebey, Lucia; Vigor, Claire; Valentin, Alexis; Moulis, Claude

2009-01-01

Tapirira guianensis is a common tree used in traditional medicine in French Guiana against several infectious diseases (malaria, leishmaniasis, bacteria, etc.). The bioassay-guided purification of CH(2)Cl(2) bark extract led to the isolation of four cyclic alkyl polyol derivatives: 4,6,2'-trihydroxy-6-[10'(Z)-heptadecenyl]-1-cyclohexen-2-one (1a), 1,4,6-trihydroxy-1,2'-epoxy-6-[10'(Z)-heptadecenyl]-2-cyclohexene (1b), 1,4,5,2'-tetrahydroxy-1-[10'(Z)-heptadecenyl]-2-cyclohexene (2), and 1,3,4,6-tetrahydroxy-1,2'-epoxy-6-[10'(Z)-heptadecenyl]-cyclohexane (3). The structures were established on the basis of 1D and 2D NMR analyses. The anti-leishmanial, anti-plasmodial, anti-bacterial (on Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli), and anti-fungal (on Candida albicans) activities of the extracts and of these original compounds were evaluated. Two showed medicinal interest supporting the traditional uses of the plant. The structures were established through spectral analyses of the isolates and their derivatives.

Marcadores para la Realidad Aumentada para fines educativos

Directory of Open Access Journals (Sweden)

María Reina Zarate Nava

2013-12-01

Full Text Available La Realidad Aumentada (AR es considerada por algunos expertos como la tecnología en crecimiento para el 2014, en diferentes sectores: ingeniería, educación, juegos y negocios, siendo este último el más usado en él 2013. Sin embargo en el sector educativo la AR se presenta en algunos proyectos que se han desarrollado para diferentes áreas de las ciencias, como Química, Física y Matemáticas, lo anterior con el objetivo de facilitar el proceso de Enseñanza-Aprendizaje. En el presente artículo se presenta el modelo de patrones utilizado para un Proyecto AR enfocado en Química, el cual presenta un conjunto de patrones que fueron valorados para facilitar el rastreo de los objetos y a su vez el entendimiento de algunos materiales de los laboratorios de Química I.

Expression of BAFF receptors in muscle tissue of myositis patients with anti-Jo-1 or anti-Ro52/anti-Ro60 autoantibodies.

Science.gov (United States)

Kryštůfková, Olga; Barbasso Helmers, Sevim; Venalis, Paulius; Malmström, Vivianne; Lindroos, Eva; Vencovský, Jiří; Lundberg, Ingrid E

2014-10-10

Anti-Jo-1 and anti-Ro52 autoantibodies are common in patients with myositis, but the mechanisms behind their production are not known. Survival of autoantibody-producing cells is dependent on B-cell-activating factor of the tumour necrosis factor family (BAFF). BAFF levels are elevated in serum of anti-Jo-1-positive myositis patients and are influenced by type-I interferon (IFN). IFN-producing cells and BAFF mRNA expression are present in myositis muscle. We investigated expression of the receptors for BAFF in muscle tissue in relation to anti-Jo-1 and anti-Ro52/anti-Ro60 autoantibodies and type-I IFN markers. Muscle biopsies from 23 patients with myositis selected based on autoantibody profile and 7 healthy controls were investigated for expression of BAFF receptor (BAFF-R), B-cell maturation antigen (BCMA) and transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI). Nineteen samples were assessed for plasma (CD138) and B-cell (CD19) markers. The numbers of positive cells per area were compared with the expression of plasmacytoid dendritic cell (pDC) marker blood dendritic cell antigen-2 (BDCA-2) and IFNα/β-inducible myxovirus resistance-1 protein (MX-1). BAFF-R, BCMA and TACI were expressed in five, seven and seven patients, respectively, and more frequently in anti-Jo-1-positive and/or anti-Ro52/anti-Ro60-positive patients compared to controls and to patients without these autoantibodies (P = BAFF-R: 0.007, BCMA: 0.03 and TACI: 0.07). A local association of receptors with B and plasma cells was confirmed by confocal microscopy. The numbers of CD138-positive and BCMA-positive cells were correlated (r = 0.79; P = 0.001). Expression of BDCA-2 correlated with numbers of CD138-positive cells and marginally with BCMA-positive cells (r = 0.54 and 0.42, respectively; P = 0.04 and 0.06, respectively). There was a borderline correlation between the numbers of positively stained TACI cells and MX-1 areas (r = 0.38, P = 0.08). The expression

Identification of {2 anti 1 anti 10} and {10 anti 10} Laue patterns of hexagonal and rhombohedral silicon carbide polytypes

International Nuclear Information System (INIS)

Yoganathan, M.; Suttrop, W.; Devaty, R.P.; Choyke, W.J.

1994-01-01

The development of epitaxic and bulk-grown semiconductor SiC exhibiting uniform polytype phase homogeneity is critically dependent upon the accurate identification of crystallographic orientations corresponding to the desired polytype. In this paper, experimental transmission Laue photographs as well as computer-generated transmission Laue patterns are presented for {2 anti 1 anti 10} and {10 anti 10} faces of the 4H, 6H and 15R polytypes of silicon carbide. The transmission patterns permit easy recognition of polytypes and crystal orientations. (orig.)

Anti- versus Pro-Competitive Mergers

OpenAIRE

Fridolfsson, Sven-Olof

2007-01-01

In a framework where mergers are mutually excluding, I show that firms pursue anti- rather than (alternative) pro-competitive mergers. Potential outsiders to anti-competitive mergers refrain from pursuing pro-competitive mergers if the positive externalities from anti-competitive mergers are strong enough. Potential outsiders to pro-competitive mergers pursue anti-competitive mergers if the negative externalities from the pro-competitive mergers are strong enough. Potential participants in an...

Anti-Coagulant and Anti-Thrombotic Properties of Blacklip Abalone (Haliotis rubra): In Vitro and Animal Studies.

Science.gov (United States)

Suleria, Hafiz Ansar Rasul; Masci, Paul P; Zhao, Kong-Nan; Addepalli, Rama; Chen, Wei; Osborne, Simone A; Gobe, Glenda C

2017-08-04

Sulphated polysaccharides with anti-thrombotic and anti-coagulant activities have been found in various marine biota. In this study, a previously characterised anti-thrombotic and anti-coagulant extract from blacklip abalone was fractionated by anion exchange chromatography (AEC), pooled (on a sulphated polysaccharide basis) and administered to Wistar rats via oral gavage (N = 8) for assessment as an oral therapeutic. To ensure that the preparation had anti-coagulant activity prior to oral administration, it was assessed in rat blood by thromboelastography (TEG) significantly increasing reaction (R) time (or time until clot formation). Following in vitro confirmation of anti-coagulant activity, 40 mg of the preparation was orally administered to rats with blood samples collected at 2, 4, and 6 h post-gavage. Assessment of all blood samples by TEG showed some prolongation of R time from 355 to 380 s after 4 h. Dosing of the post-gavage blood samples with the abalone preparation to confirm anti-thrombotic activity in vitro revealed residual anti-coagulant activity, further suggesting that oral administration did increase anti-coagulant potential in the collected blood but that bioavailability was low. Assessment of tissues and haematological parameters showed no obvious harmful effects of the abalone preparation in animals. In summary, even though oral administration of fractionated and pooled blacklip abalone extract to rats delayed clotting after 4 h, bioavailability of the preparation appeared to be low and may be more appropriate for intravenous administration as an anti-thrombotic or anti-coagulant therapeutic.

Generating para-water from para-hydrogen: A Gedankenexperiment.

Science.gov (United States)

Ivanov, Konstantin L; Bodenhausen, Geoffrey

2018-07-01

A novel conceptual approach is described that is based on the transfer of hyperpolarization from para-hydrogen in view of generating a population imbalance between the two spin isomers of H 2 O. The approach is analogous to SABRE (Signal Amplification By Reversible Exchange) and makes use of the transfer of spin order from para-hydrogen to H 2 O in a hypothetical organometallic complex. The spin order transfer is expected to be most efficient at avoided level crossings. The highest achievable enrichment levels of para- and ortho-water are discussed. Copyright © 2018 Elsevier Inc. All rights reserved.

Oxidized LDL-induced angiogenesis involves sphingosine 1-phosphate: prevention by anti-S1P antibody.

Science.gov (United States)

Camaré, Caroline; Trayssac, Magali; Garmy-Susini, Barbara; Mucher, Elodie; Sabbadini, Roger; Salvayre, Robert; Negre-Salvayre, Anne

2015-01-01

Neovascularization occurring in atherosclerotic lesions may promote plaque expansion, intraplaque haemorrhage and rupture. Oxidized LDL (oxLDL) are atherogenic, but their angiogenic effect is controversial; both angiogenic and anti-angiogenic effects have been reported. The angiogenic mechanism of oxLDL is partly understood, but the role of the angiogenic sphingolipid, sphingosine 1-phosphate (S1P), in this process is not known. Thus, we investigated whether S1P is involved in the oxLDL-induced angiogenesis and whether an anti-S1P monoclonal antibody can prevent this effect. Angiogenesis was assessed by capillary tube formation by human microvascular endothelial cells (HMEC-1) cultured on Matrigel and in vivo by the Matrigel plug assay in C57BL/6 mice. Human oxLDL exhibited a biphasic angiogenic effect on HMEC-1; low concentrations were angiogenic, higher concentrations were cytotoxic. The angiogenic response to oxLDL was blocked by the sphingosine kinase (SPHK) inhibitor, dimethylsphingosine, by SPHK1-siRNA and by an anti-S1P monoclonal antibody. Moreover, inhibition of oxLDL uptake and subsequent redox signalling by anti-CD36 and anti-LOX-1 receptor antibodies and by N-acetylcysteine, respectively, blocked SPHK1 activation and tube formation. In vivo, in the Matrigel plug assay, low concentrations of human oxLDL or murine oxVLDL also triggered angiogenesis, which was prevented by i.p. injection of the anti-S1P antibody. These data highlight the role of S1P in angiogenesis induced by oxLDL both in HMEC-1 cultured on Matrigel and in vivo in the Matrigel plug model in mice, and demonstrate that the anti-S1P antibody effectively blocks the angiogenic effect of oxLDL. © 2014 The British Pharmacological Society.

Toxicidad oral a 60 días del aceite de sacha inchi (Plukenetia volubilis L. y linaza (Linum usitatissimum L. y determinación de la dosis letal 50 en roedores Oral toxicity at 60-days of sacha inchi oil (Plukenetia volubilis L. and linseed (Linum usitatissimum L., and determination of lethal dose 50 in rodents

Directory of Open Access Journals (Sweden)

Arilmi Gorriti

2010-09-01

Full Text Available Objetivos. Evaluar la toxicidad oral a 60 días y determinar la dosis letal 50 (DL 50 de los aceites crudos de sacha inchi (Plukenetia volubilis L. y linaza (Linum ussitatisimum en ratas Holtzman y en ratones cepa Balb C57, respectivamente. Materiales y métodos. Para la evaluación de la toxicidad oral a dosis repetida por 60 días se utilizó 24 ratas macho Holtzman divididos en tres grupos de ocho cada uno, los grupos fueron: solución salina fisiológica 4 mL/kg (SSF, aceite de sacha inchi 0,5 mL/kg (SI05 y aceite de linaza 0,5 mL/kg (L05, durante el experimento se controló semanalmente el peso corporal y signos de toxicidad en los grupos investigados, así como colesterol total, HDL, triglicéridos, glucosa, urea, TGP y fosfatasa alcalina a los 30 y 60 días de iniciado el experimento. Para la evaluación de la DL50 se usó ratones macho cepa Balb C57 en grupos de diez animales, se administró por vía oral dosis crecientes de aceites crudos hasta alcanzar 1 mL/kg (37 g/kg; Resultados. Los parámetros séricos en las ratas indican que no existe toxicidad alguna a los 60 días y que la administración de los aceites disminuyeron los niveles de colesterol, triglicéridos e incrementaron el HDL con respecto al grupo control. La DL50 muestra que los aceites crudos de sacha inchi y linaza presentan dosis por encima de los 37 g/kg de masa corporal. Conclusiones. Los aceites de sacha inchi y linaza son inocuos a 60 días y presentan una DL50 por encima de los 37 g/kg de animal.Objectives. To evaluate the oral toxicity at 60 days and to determine the lethal dose 50 (LD 50 of raw sacha inchi (Plukenetia volubilis L. and linseed (Linum ussitatisimum oils in Holtzman rats and mice of the strain Balb C57 respectively. Materials and methods. For the evaluation of the oral toxicity of repeated doses for 60 days, 24 male Holtzman rats were used, divided in three groups of 8 each, the groups were: physiologic saline solution 4 mL/kg (FSS, sacha inchi oil

Rituximab for the treatment of refractory simultaneous anti-glomerular basement membrane (anti-GBM) and membranous nephropathy.

Science.gov (United States)

Bandak, Ghassan; Jones, Bruce A; Li, Jian; Yee, Jerry; Umanath, Kausik

2014-02-01

Antibody-mediated anti-glomerular basement membrane (anti-GBM) disease occurs rarely in the presence of another B-cell disorder, membranous nephropathy. The coexistence of these two autoimmune disorders would be anticipated to require differing, specific therapies targeted to each disease process. We describe a case of concomitant membranous nephropathy and anti-GBM disease in which conventional therapy, including steroids, plasmapheresis and cyclophosphamide, failed to attenuate the anti-GBM disease, yet responded to an alternative treatment of rituximab. This B-cell directed, monoclonal, chimeric antibody treatment substantially reduced anti-GBM antibody titers and led to discontinuation of plasmapheresis, while maintaining the remission of membranous nephropathy and anti-GBM disease.

anti p and anti d production in relativistic heavy ion collisions: Results of BNL-E858

Energy Technology Data Exchange (ETDEWEB)

Stankus, P; Nagamiya, S [Columbia Univ., Nevis Labs., Irvington, NY (United States); Aoki, M; Hayano, R S; Shimizu, Y [Tokyo Univ. (Japan); Beatty, J [Boston Univ., MA (United States); Beavis, D; Debbe, R [Brookhaven National Lab., Upton, NY (United States); Carroll, J B [California Univ., Los Angeles, CA (United States); Chiba, J; Tanaka, K H [National Lab. for High Energy Physics (KEK), Tsukuba (Japan); Doke, T; Kashiwagi, T; Kikuchi, J [Waseda Univ., Tokyo (Japan); Hallman, T J [Johns Hopkins Univ., Baltimore, MD (United States); Heckman, H H; Lindstrom, P J [Lawrence Berkeley Lab., CA (United States); Kirk, P N; Wang, Z F [Louisiana State Univ., Baton Rouge, LA (United States); Crawford, H J; Engelage, J; Greiner, L [Space Sciences Lab., California Univ., Berkeley, CA (United States); E858 Collaboration

1992-07-20

The production of long-lived negative secondaries, including [pi][sup -], K[sup -], antiprotons (anti p) and antideuterons (anti d) at 0deg in Si+Au, Si+Cu, and Si+Al collisions at the BNL-AGS has been measured using a double-bend, double-focussing spectrometer. The anti p rapidity spectra from all three targets are well fit by Gaussians centered (anti y = 1.7 - 1.8) at mid-rapidity, and are consistent with production in nucleon-nucleon first collisions. The anti d/anti p[sup 2] ratio in Si+Al collisions is lower by a factor of 10 than that seen in p+p and p+A collisions. (orig.).

Anti-atherosclerotic effects of tomatoes

Directory of Open Access Journals (Sweden)

Hidekatsu Yanai

2017-06-01

Full Text Available Tomatoes are rich in lycopene, which causes the red coloring of tomatoes. Several reports have suggested lycopene plays a role in the prevention of cardiovascular diseases. In this study, we systematically reviewed the interventional studies using tomatoes or tomato products to understandtheanti-atherosclerotic effects of the tomatoas a functional food. We found that a significantnumber of interventional studies reportedtheanti-atherosclerotic effects of tomatoes, includinganti-obesity effects, hypotensiveeffects, improvement of lipid/glucose metabolismand endothelial function, anti-oxidative and anti-inflammatory effect, and anti-platelet effect; however, the anti-platelet effect was disagreed uponby some studies. Furthermore, we discoveredcooking methods significantlyaffect anti-atherosclerotic effects of tomatoes.

Evaluación de la Actividad Resortiva de Macrófagos de Línea Celular Humana y de Ratón Fusionados con PEG

OpenAIRE

Carlos A. Guerrero; M. Andrea. Murillo; Carmen. A. Cardozo de Martinez

2005-01-01

Premio Aventis – Ciencias Básicas y Experimentales. LIBROS DE MEDICINA. Los osteoclastos son células grandes multinucleadas, tienen diferentes características que incluyen la expresión de la fosfatasa ácida resistente al tartrato (TRAP), receptor de calcitonina (RCT) y la capacidad de resorber hueso. Su origen no se ha dilucidado plenamente, sin embargo hay evidencias que sugieren que los osteoclastos proceden de la fusión de precursores mononucleares pertene...

RESPOSTAS DA ATIVIDADE ENZIMÁTICA HEPÁTICA E CEREBRAL EM FUNÇÃO DE DIFERENTES NÍVEIS DE RESTRIÇÃO ALIMENTAR: I – FOSFATASE

Directory of Open Access Journals (Sweden)

Olga Maria Mascarenhas Faria de Oliveira

2007-01-01

Full Text Available O estudo foi feito para determinar a influência de restrição alimentar estabelecida em vários níveis sobre a atividade enzimática de algumas enzimas. Foram alimentados ratos machos com dieta comercial ad libitum (grupo controle e com restrição de 30% e 40% (grupos experimentais. Após o período experimental que teve duração de 28 dias, os animais foram sacrificados e os fígados, cérebros e rins removidos, homogeneizados e submetidos a diferentes velocidades de centrifugação e, em cada etapa foram realizados estudos cinético-enzimáticos das referidas enzimas e dosagem protéica. Os resultados mostraram que a restrição alimentar afeta a quantidade das enzimas fosfatase alcalina e amilase, além do conteúdo protéico, levando a significativo aumento ou diminuição de acordo com o grau de restrição e com a fase do fracionamento centrífugo estudada. O mesmo observou-se para o exercício físico.

Quantitation of anti-tetanus and anti-diphtheria antibodies by enzymoimmunoassay: methodology and applications.

Science.gov (United States)

Virella, G; Hyman, B

1991-01-01

We have developed enzymoimmunoassays (EIA) for the quantitation of antibodies (Ab) to tetanus and diphtheria toxoids (TT, DT) using Immulon I plates coated with the appropriate toxoid. A preparation of human tetanus immunoglobulin with a known concentration of anti-TT Ab was used as calibrator of the anti-TT antibody assay. The assay of anti-DT Ab is calibrated with a pool of human sera whose anti-DT Ab concentration was determined by quantitative immunoelectrophoresis, using a horse anti-DT with known Ab concentration as calibrator. A peroxidase-conjugated anti-human IgG was used in both assays. ABTS was used as substrate, and the reaction was stopped after 1 min incubation with citric acid and the OD measured at 414 nm on a Vmax reader. The assays have been applied to a variety of clinical situations. In patients suspected of having tetanus, the quantitation of antibodies has been helpful in establishing a diagnosis. In patients with a history of hypersensitivity to tetanus toxoid, verification of the levels of anti-TT antibody may prevent unnecessary and potentially harmful immunizations. The assays have also been used for the diagnostic evaluation of the humoral immune response to TT and DT, both in pediatric patients and in immunosuppressed patients. Several non-responders have been detected, and we have recently used the assay to monitor the effects of fish oil administration on the humoral immune response.(ABSTRACT TRUNCATED AT 250 WORDS)

A short history of anti-rheumatic therapy - V. Analgesics

Directory of Open Access Journals (Sweden)

P. Marson

2011-06-01

Full Text Available The pharmacological treatment of pain has very ancient origins, when plant-derived products were used, including mandrake extracts and opium, a dried latex obtained from Papaver somniferum. In the XVI and XVII centuries opium came into the preparation of two compounds widely used for pain relief: laudanum and Dover’s powder. The analgesic properties of extracts of willow bark were then recognized and later, in the second half of the XIX century, experimental studies on chemically synthesized analgesics were planned, thus promoting the marketing of some derivatives of para-amino-phenol and pyrazole, the predecessors of paracetamol and metamizol. In the XX century, nonsteroidal anti-inflammatory drugs were synthesized, such as phenylbutazone, which was initially considered primarily a pain medication. The introduction on the market of centrally acting analgesics, such as tramadol, sometimes used in the treatment of rheumatic pain. is quite recent.

Predictions for the anti B0 → anti K*0 X(YZ) and anti Bs0 → φ X(YZ) with X(4160), Y(3940), Z(3930)

International Nuclear Information System (INIS)

Liang, Wei-Hong; Molina, R.; Doering, M.; Xie, Ju-Jun; Oset, E.

2015-01-01

We investigate the decay of anti B 0 → anti K *0 R and anti B s 0 → φR with R being the X(4160), Y(3940), Z(3930) resonances. Under the assumption that these states are dynamically generated from the vector-vector interaction, as has been concluded from several theoretical studies, we use a reaction mechanism of quark production at the elementary level, followed by hadronization of one final q anti q pair into two vectors and posterior final state interaction of this pair of vector mesons to produce the resonances. With this procedure we are able to predict five ratios for these decays, which are closely linked to the dynamical nature of these states, and also predict the order of magnitude of the branching ratios which we find of the order of 10 -4 , well within the present measurable range. In order to further test the dynamical nature of these resonances we study the anti B s 0 → φ D* anti D* and anti B s 0 → φ D s * anti D s * decays close to the D* anti D* and D s * anti D s * thresholds and make predictions for the ratio of the mass distributions in these decays and the anti B s 0 → φR decay widths. The measurement of these decays rates can help unravel the nature of these resonances. (orig.)

Nature is the best source of anti-inflammatory drugs: indexing natural products for their anti-inflammatory bioactivity.

Science.gov (United States)

Aswad, Miran; Rayan, Mahmoud; Abu-Lafi, Saleh; Falah, Mizied; Raiyn, Jamal; Abdallah, Ziyad; Rayan, Anwar

2018-01-01

The aim was to index natural products for less expensive preventive or curative anti-inflammatory therapeutic drugs. A set of 441 anti-inflammatory drugs representing the active domain and 2892 natural products representing the inactive domain was used to construct a predictive model for bioactivity-indexing purposes. The model for indexing the natural products for potential anti-inflammatory activity was constructed using the iterative stochastic elimination algorithm (ISE). ISE is capable of differentiating between active and inactive anti-inflammatory molecules. By applying the prediction model to a mix set of (active/inactive) substances, we managed to capture 38% of the anti-inflammatory drugs in the top 1% of the screened set of chemicals, yielding enrichment factor of 38. Ten natural products that scored highly as potential anti-inflammatory drug candidates are disclosed. Searching the PubMed revealed that only three molecules (Moupinamide, Capsaicin, and Hypaphorine) out of the ten were tested and reported as anti-inflammatory. The other seven phytochemicals await evaluation for their anti-inflammatory activity in wet lab. The proposed anti-inflammatory model can be utilized for the virtual screening of large chemical databases and for indexing natural products for potential anti-inflammatory activity.

The Modern Anti-Semitism Israel Model: An empirical relationship between modern anti-Semitism and opposition to Israel

Directory of Open Access Journals (Sweden)

Florette Cohen

2011-04-01

Full Text Available The current paper reviews our program of research that has examined some of the causes and consequences of anti-Semitism in which a new theoretical model of anti-Semitism is presented and tested in six experiments. The model proposes that mortality salience increases anti-Semitism and that anti-Semitism often manifests as hostility towards Israel. In accord with predictions, results show that existential fears lead to higher anti-Semitism and reduced support for Israel. Collectively, these results may serve as a preliminary contribution to explaining the continuation of anti-Semitism.

Auto-immune anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis: three case reports.

Science.gov (United States)

Bashiri, Fahad A; Al-Rasheed, Abdulrahman A; Hassan, Saeed M; Hamad, Muddathir H A; El Khashab, Heba Y; Kentab, Amal Y; AlBadr, Fahad B; Salih, Mustafa A

2017-08-01

Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis is a recently identified auto-immune disorder characterised by severe memory deficit, a decreased level of consciousness, seizures, autonomic dysfunction and movement disorders. Three girls with the disorder are reported; they were aged 4 years, 5 years and 10 months. The 10-month-old infant who is one of the youngest patients reported with anti-NMDAR encephalitis worldwide, had MRI features suggestive of herpes simplex encephalitis (known to trigger anti-NMDAR encephalitis), but CSF PCR for herpes simplex was negative. All the patients presented with seizures, behavioural change, regression of speech, dystonia and choreo-athetosis. Anti-NMDAR antibodies were detected in all patients' sera and cerebrospinal fluid (CSF). Intravenous immunoglobulin, corticosteroids and rituximab were administered at different intervals. Cases 1 and 2 made a full recovery, but case 3 has mild motor and speech delay. Patients who present with encephalopathy, seizures and movement disorders should be tested for anti-NMDAR antibodies in serum and CSF in addition to being screened for herpes simplex encephalitis.

The Anti-TNF-α Therapy in the Rheumatoid Arthritis A Terapia Anti-TNF-α na Artrite Reumatóide

Directory of Open Access Journals (Sweden)

Lilian Resende Faleiro

2011-06-01

, estão disponíveis no mercado brasileiro, três agentes anti-TNF-α: infliximabe, etanercepte e adalimumabe. Estes agentes são relativamente seguros para Artrite Reumatóide, mas podem, no entanto, apresentar complicações infecciosas graves, como a reativação da tuberculose latente. O alto custo dessas drogas, seu uso em nível hospitalar e o risco a infecções oportunistas permanecem como fatores limitantes para sua ampla utilização no tratamento da Artrite Reumatóide em nosso meio. Este estudo tem como objetivo destacar a importância do surgimento de novas terapias com intuito de atenuar a progressão da Artrite Reumatóide, bem como analisar risco-benefício oferecidos pelo respectivo tratamento e ainda avaliar viabilidade e custo do mesmo.

Grado de cumplimiento de la legislación e impartición de enseñanzas anti-tabaco en los centros escolares españoles

Directory of Open Access Journals (Sweden)

Hernández-Mezquita Miguel Angel

2000-01-01

Full Text Available Fundamento: No se dispone de estudios que analicen el cumplimiento de la legislación antitabaco en los centros escolares españoles, por lo que nos propusimos conocer el grado de cumplimiento de la legislación oficial anti-tabaco en dichos centros y la influencia que ello tiene sobre el porcentaje de alumnos fumadores. Método: Se realizó una encuesta entre 3.050 directores de centros educativos españoles, a los que se les preguntó sobre diversos aspectos del cumplimiento de la legislación oficial anti-tabaco en los centros y sobre las principales características del consumo de tabaco en los mismos. Resultados: El 80,9% de los directores afirman que en sus centros se exige el cumplimiento de la legislación y el 64,9% disponen de carteles que advierten de la prohibición de fumar. Afirman impartir enseñanzas anti-tabaco el 82,2% de los centros en los que se exige la legislación y en el 64,8% de los centros en los que esta legislación no se exige. Creen que el porcentaje de profesores que fuman ante los alumnos es del 5,9% en los centros en que se exige la legislación y del 12,9% en los que no se cumple. En los centros donde se imparten enseñanzas anti-tabaco creen que han probado el tabaco el 29% de los alumnos, frente al 31% que creen lo han hecho en los centros donde no se imparten estas enseñanzas (p<0,001. Conclusiones: El cumplimiento de la legislación es insuficiente. Los directores de los centros educativos, como encargados de velar por el cumplimiento de la legislación oficial anti-tabaco en sus centros, deben exigir el cumplimiento de esta legislación, ya que esta medida es eficaz para mejorar el funcionamiento global de los centros y para reducir los porcentajes de alumnos y profesores fumadores.

Seropositivity of HBsAg, anti-HCV and anti-HIV in preoperative patients

Directory of Open Access Journals (Sweden)

Berrin Karaayak Uzun

2014-12-01

Full Text Available Objective: The infections caused by human immunodeficiency virus (HIV, hepatitis B (HBV and C (HCV viruses pose a serious occupational risk for the healthcare workers especially those in emergency services, laboratories and surgery wards. Vaccination and establishment of the strict biosafety procedures are the main principles to prevent blood-borne infections in healthcare workers. Additionally, serological screening of the preoperative patients could decrease the risk for exposure. In this study, we aimed to determine the seroprevalence of HBsAg, anti-HCV, anti-HIV 1/2 in preoperative patients. Methods: Hospital automation records were evaluated retrospectively for 4.367 patients who were scheduled for surgery and scanned for anti-HIV 1/2, HBsAg and anti-HCV as preoperative procedures in the preparation period of operation between January 2012 and December 2012. Results: HBsAg positivity rate was found in 7.7% (n=336, anti-HCV positivity rate was found in 2.3% (n=101. A two (0.05% of five patients were positive for anti-HIV 1/2 was found positive verification test and the other three samples were accepted as false positive test results. Conclusion: All healthcare workers must be trained about occupational diseases and vaccinated against Hepatitis B. Universal precautions must be strictly followed particularly in the operating room. In addition, all patients should be considered as potential carriers regarded as a carrier of the potential for infection. J Clin Exp Invest 2013; 4 (4: 449-452

Uma ferramenta para planejamento de estudos para concursos

Directory of Open Access Journals (Sweden)

Leonardo Pimentel

2015-11-01

Full Text Available De acordo com a constituição brasileira, a única forma de se tornar um empregado do governo é passar em um concurso público. O exame de conhecimento do cargo é um dos componentes mais importantes de um concurso. Estudar para esse exame é uma tarefa que requer planejamento. Um meio de obter um bom planejamento é construir uma grade de horários que relacione as matérias a serem estudadas com os respectivos horários disponíveis para o estudo. Esse tipo de problema se assemelha bastante à gerência de projetos, em que se procura estabelecer a distribuição das atividades ao longo do tempo disponível. Assim, torna-se interessante explorar a dinâmica de estudos para concursos como um projeto. O presente trabalho apresenta a ferramenta de apoio ao estudos - Passe-me, baseada no ciclo PDCA (do inglês, Plan-Do-Check-Act. Por se tratar de uma ferramenta web, o tempo de resposta para produzir uma grade de estudos é fundamental. A ferramenta faz uso de algoritmo genético para calcular a grade de horários priorizando a velocidade de resposta. A ferramenta foi avaliada em diferentes cenários de uso e obteve desempenho satisfatório durante a produção da grade de estudos.

Docencia para el logro y educación para emprender

Directory of Open Access Journals (Sweden)

Luis Gerardo Rodríguez Gómez

1994-05-01

Full Text Available RESUMEN En el primer artículo, el autor plasmó los elementos relevantes para una propuesta de educación para emprender. En este describe el desarrollo docente como expresión de una intensión, saber y un quehacer, encaminados a la formación del hombre emprendedor. El artículo se divide en tres partes. En la primera, se reseña los factores que orientan la intensión docente para emprender, a partir de un discurso y formas metodológicas establecidas con esa intención. En segundo lugar, se asume el espacio y tiempo la clase como unidad de análisis, para lograr objetivos de tipo institucional, programático y temático y en la parte final, se examina la evaluación docente y se hacen consideraciones generales para una propuesta de evaluación,  que surge al investigar la actuación del maestro.

Anti-tumor and anti-angiogenic ergosterols from Ganoderma lucidum

Science.gov (United States)

Chen, Shaodan; Yong, Tianqiao; Zhang, Yifang; Su, Jiyan; Jiao, Chunwei; Xie, Yizhen

2017-10-01

This study was carried out to isolate chemical constituents from the lipid enriched fraction of Ganoderma lucidum extract and to evaluate their anti-proliferative effect on cancer cell lines and human umbilical vein endothelial cells. Ergosterol derivatives (1-14) were isolated from the lipid enriched fraction of G. lucidum. Their structures were established on the basis of spectroscopic analyses or by comparison of mass and NMR spectral data with those reported previously. Amongst, compound 1 was isolated and identified as a new compound. All the compounds were evaluated for their inhibitory effect on tumor cells and human umbilical vein endothelial cells in vitro. Compounds 9-13 displayed inhibitory activity against two tumor cell lines and human umbilical vein endothelial cells, which indicated that these four compounds had both anti-tumor and anti-angiogenesis activities. Compound 2 had significant selective inhibition against two tumor cell lines, while 3 exhibited selective inhibition against human umbilical vein endothelial cells. The structure–activity relationships for inhibiting human HepG2 cells were revealed by 3D-QASR. Ergosterol content in different parts of the raw material and products of G. lucidum was quantified. This study provides a basis for further development and utilization of ergosterol derivatives as natural nutraceuticals and functional food ingredients, or as source of new potential antitumor or anti-angiogenesis chemotherapy agent.

Docking Studies, Synthesis and Biological Evaluation of β-aryl-β-hydroxy Propanoic Acids for Anti-inflammatory Activity.

Science.gov (United States)

Savic, Jelena; Dilber, Sanda; Milenkovic, Marina; Kotur-Stevuljevic, Jelena; Markovic, Bojan; Vladimirov, Sote; Brboric, Jasmina

2017-01-01

Nonsteriodal anti-inflammatory drugs (NSAIDs) are numerous and widely used for more than 60 years, but there is still a strong need for developing novel selective NSAIDs. The need is justified by the fact that nonselective NSAIDs can produce serious gastric side effects and that some of the selective NSAID are withdrawn due to their cardiotoxic side effects. Eight β-hydroxy-β-arylpropanoic acids, which belong to the arylpropanoic acid class of compounds, structurally similar to some nonsteroidal anti-inflammatory drugs (NSAIDs), were docked into 3D catalytic site of both cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Seven out of those eight acids were synthesized using already published modification of Reformatsky reaction additionally optimized by increasing temperature. Synthesized compounds were tested in vivo in order to elucidate anti-inflammatory activity, gastric tolerability and impact on liver function of rats. Results of docking studies have indicated that all compounds have potential to selectively inhibit COX-2 isoform, but that the compounds containing polar substituents on phenyl ring are better inhibitors. Results of carrageenan-induced rat paw oedema test have shown that all compounds exhibit dose dependence and good gastric tolerability and none of the tested compounds have shown negative effect on liver function compared to ibuprofen. The compound containing polar nitro group in para position has shown the best docking results, anti-inflammatory activity, low hepatotoxicity and good gastric tolerability. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Tropical fruit camu-camu (Myrciaria dubia) has anti-oxidative and anti-inflammatory properties.

Science.gov (United States)

Inoue, Teruo; Komoda, Hiroshi; Uchida, Toshihiko; Node, Koichi

2008-10-01

Oxidative stress as well as inflammation plays a pivotal role in the pathogenesis of atherosclerosis. Although, various anti-oxidative dietary supplements have been evaluated for their ability to prevent atherosclerosis, no effective ones have been determined at present. "Camu-camu" (Myrciaria dubia) is an Amazonian fruit that offers high vitamin C content. However, its anti-oxidative property has not been evaluated in vivo in humans. To assess the anti-oxidative and anti-inflammatory properties of camu-camu in humans, 20 male smoking volunteers, considered to have an accelerated oxidative stress state, were recruited and randomly assigned to take daily 70 ml of 100% camu-camu juice, corresponding to 1050 mg of vitamin C (camu-camu group; n=10) or 1050 mg of vitamin C tablets (vitamin C group; n=10) for 7 days. After 7 days, oxidative stress markers such as the levels of urinary 8-hydroxy-deoxyguanosine (P<0.05) and total reactive oxygen species (P<0.01) and inflammatory markers such as serum levels of high sensitivity C reactive protein (P<0.05), interleukin (IL)-6 (P<0.05), and IL-8 (P<0.01) decreased significantly in the camu-camu group, while there was no change in the vitamin C group. Our results suggest that camu-camu juice may have powerful anti-oxidative and anti-inflammatory properties, compared to vitamin C tablets containing equivalent vitamin C content. These effects may be due to the existence of unknown anti-oxidant substances besides vitamin C or unknown substances modulating in vivo vitamin C kinetics in camu-camu.

Possible D(*) anti D(*) and B(*) anti B(*) molecular states in the extended constituent quark models

International Nuclear Information System (INIS)

Yang, You-Chang; Tan, Zhi-Yun; Ping, Jialun; Zong, Hong-Shi

2017-01-01

The possible neutral D (*) anti D (*) and B (*) anti B (*) molecular states are studied in the framework of the constituent quark models, which is extended by including the s-channel one-gluon exchange. Using different types of quark-quark potentials, we solve the four-body Schroedinger equation by means of the Gaussian expansion method. The bound states of D (*) anti D (*) with J PC = 1 ++ , 2 ++ and B (*) anti B (*) with J PC = 0 ++ , 1 +- , 1 ++ , 2 ++ are obtained. The molecular states D* anti D with J PC = 1 ++ and B* anti B with J PC = 1 +- are good candidates for X(3872) and Z 0 b (10610), respectively. The dependence of the results on the model parameters is also discussed. (orig.)

Walloons as General or Specific Others? A Comparison of anti-Walloon and anti-immigrant Attitudes in Flanders

Directory of Open Access Journals (Sweden)

Bart Meuleman

2017-11-01

Full Text Available This study attempts to shed light on the structure, the prevalence and the determinants of anti-Walloon attitudes in Flanders. For this purpose, we contrast anti-Walloon prejudice with prejudice against a relatively well-understood and archetypical out-group, namely immigrants. Our theoretical approach draws on insights from two paradigms of intergroup relations: the Group-Focused Enmity approach stressing that specific prejudices have a strong common denominator, and the Differentiated Threat model arguing that specific prejudices are contingent on the context of intergroup relations as well as the involved types of threat. To assess the (dissimilarities in anti-Walloon and anti-immigrant prejudice, we use the Flemish dataset of the Belgian National Election Study (BNES 2010. Comparable measurement instruments for both forms of prejudice are analyzed by means of structural equation modeling. Our results reveal a nuanced picture regarding the similarities and differences between anti-Walloon and anti-immigrant attitudes in Flanders. One the one hand, anti-Walloon and anti-immigration attitudes are strongly correlated and rooted in economic threat perceptions. On the other hand, anti-Walloon attitudes are less outspoken in the Flemish population than anti-immigrant attitudes, are less founded on cultural threat perceptions and are more closely linked to feelings of identification with the Flemish in-group.

Avaliação Comparativa de Teste Imunocromatográfico para Identificação Forense de Sangue Humano

Directory of Open Access Journals (Sweden)

Paulo Eduardo Longo

2011-09-01

Full Text Available A determinação de especificidade humana pode indicar a presença de sangue humano, mesmo em amostras antigas provindas de investigações criminais, contaminadas ou em estado de putrefação. O presente estudo visa analisar se há coerência entre os resultados obtidos pelos testes de orientação e específico para sangue humano utilizados rotineiramente na análise forense em comparação com o teste imunocromatográfico para detecção da hemoglobina humana. O diagnóstico por meio do anticorpo anti-hemoglobina humana tem se mostrado eficiente na detecção qualitativa de hemoglobina humana em amostras de sangue depositadas em peças e locais de crime.

Neostigmine interactions with non steroidal anti-inflammatory drugs.

Science.gov (United States)

Miranda, Hugo F; Sierralta, Fernando; Pinardi, Gianni

2002-04-01

1. The common mechanism of action of non-steroidal anti-inflammatory drugs (NSAIDs) is the inhibition of the enzyme cyclo-oxygenase (COX), however, this inhibition is not enough to completely account for the efficacy of these agents in several models of acute pain. 2. It has been demonstrated that cholinergic agents can induce antinociception, but the nature of the interaction between these agents and NSAIDs drugs has not been studied. The present work evaluates, by isobolographic analysis, the interactions between the cholinergic indirect agonist neostigmine (NEO) and NSAIDs drugs, using a chemical algesiometric test. 3. Intraperitoneal (i.p.) or intrathecal (i.t.) administration of NEO and of the different NSAIDs produced dose-dependent antinociception in the acetic acid writhing test of the mouse. 4. The i.p. or i.t. co-administration of fixed ratios of ED(50) fractions of NSAIDs and NEO, resulted to be synergistic or supra-additive for the combinations ketoprofen (KETO) and NEO, paracetamol (PARA) and NEO) and diclofenac (DICLO) and NEO administered i.p. However, the same combinations administered i.t. were only additive. In addition, the combinations meloxicam (MELO) and NEO and piroxicam (PIRO) and NEO, administered either i.p. or i.t., were additive. 5. The results suggest that the co-administration of NEO with some NSAIDs (e.g. KETO, PARA or DICLO) resulted in a synergistic interaction, which may provide evidence of supraspinal antinociception modulation by the increased acetylcholine concentration in the synaptic cleft of cholinergic interneurons. The interaction obtained between neostigmine and the NSAIDs could carry important clinical implications.

The anti-proliferative and anti-angiogenic effect of the methanol extract from brittle star.

Science.gov (United States)

Baharara, Javad; Amini, Elaheh; Mousavi, Marzieh

2015-04-01

Anti-angiogenic therapy is a crucial step in cancer treatment. The discovery of new anti-angiogenic compounds from marine organisms has become an attractive concept in anti-cancer therapy. Because little data correlated to the pro- and anti-angiogenic efficacies of Ophiuroidea, which include brittle star, the current study was designed to explore the anti-angiogenic potential of brittle star methanol extract in vitro and in vivo. The anti-proliferative effect of brittle star extract on A2780cp cells was examined by MTT assays, and transcriptional expression of VEGF and b-FGF was evaluated by RT-PCR. In an in vivo model, 40 fertilized Ross eggs were divided into control and three experimental groups. The experimental groups were incubated with brittle star extract at concentrations of 25, 50 and 100 µg/ml, and photographed by photo-stereomicroscopy. Ultimately, numbers and lengths of vessels were measured by Image J software. Data were analyzed with SPSS software (pstar extract exerted a dose- and time-dependent anti-proliferative effect on A2780cp cancer cells. In addition, VEGF and b-FGF expression decreased with brittle star methanol extract treatment. Macroscopic evaluations revealed significant changes in the second and third experimental group compared to controls (pstar methanol extract in vitro and in vivo confer novel insight into the application of natural marine products in angiogenesis-related pathologies.

AVALIAÇÃO DA DISPENSAÇÃO DE FÁRMACOS PARCIALMENTE SELETIVOS PARA COX-2

Directory of Open Access Journals (Sweden)

Cícero Cardoso VALLE FILHO

2015-12-01

Full Text Available Uma pesquisa foi realizada com o objetivo de avaliar um potencial aumento na venda de fármacos anti-inflamatórios não esteroidais (AINEs parcialmente seletivos para a COX-2 durante o período de julho de 2009 a junho de 2011, em uma drogaria localizada no município de Itaperuna, estado do Rio de Janeiro. Durante o período estudado foi dispensado um total de 802 AINEs parcialmente seletivos para a COX-2, dentre os quais 59,85% foram de nimesulida, 5,74% de etodolaco e 34,41% de meloxicam.   No primeiro semestre avaliado a dispensação total de AINEs parcialmente seletivos foi equivalente a 92, no segundo semestre a dispensação total foi equivalente a 159, no terceiro semestre a dispensação total foi de 219 AINEs parcialmente seletivos para COX-2 e no quarto semestre foram dispensados 332.  Os resultados indicaram maior dispensação dos medicamentos desta classe no mês de abril de 2011 sendo que tanto a nimesulida, quanto o meloxicam e o etodolaco foram mais dispensados no último semestre do estudo. De acordo com a metodologia utilizada pode-se concluir que a dispensação dos AINEs parcialmente seletivos vem crescendo a cada ano e que a nimesulida apresenta o maior índice de crescimento; a maior indicação da nimesulida tem sido para crianças; os meses correspondentes ao período de inverno apresentam maior dispensação de AINEs parcialmente seletivos para a COX-2.

Anti-smooth muscle antibody

Science.gov (United States)

... gov/ency/article/003531.htm Anti-smooth muscle antibody To use the sharing features on this page, please enable JavaScript. Anti-smooth muscle antibody is a blood test that detects the presence ...

Indicaciones para el uso de los inhibidores de la PCSK9

Directory of Open Access Journals (Sweden)

Rodrigo Alonso

2017-11-01

Full Text Available Los anticuerpos monoclonales anti PCSK9, evolocumab y alirocumab, representan un nuevo grupo de hipolipemiantes no-estatinas, destinados a conseguir reducciones significativas en el cLDL, específicamente en pacientes de alto riesgo cardiovascular que no logran las metas en el cLDL propuestas en la actualidad a pesar de cambios en el estilo de vida, tratamiento máximo tolerado de estatinas en monoterapia o combinadas con ezetimibe, así como en aquellos que son intolerantes o tienen contraindicación para el uso de estatinas pero que igualmente tienen alto riesgo de eventos cardiovasculares. La evidencia clínica demuestra que los i. PCSK9 son fármacos eficaces y seguros, que reducen los eventos cardiovasculares.

anti K-nucleon interactions

Energy Technology Data Exchange (ETDEWEB)

Chand, Ramesh

1963-10-15

Total scattering and absorption cross sections for anti K-nucleon collisions in I = 1, p_3/2 - channel are given as functions of the two sets of energy dependent anti KN scattering parameters solutions, called solution A' and solution B'. These scattering parameters are obtained by linear interpolations between Watson's amplitudes around 400 MeV/c and the amplitude at the position of the pole in the anti KN scattering amplitude corresponding to the p_3/2-wave 1385 MeV Y₁-resonance with 50 MeV width. The zero-range expansion for p-wave anti K-nucleon phase shift and the scattering parameters of Watson's solution B are found to be in violation of the requirements of causality and of positive definiteness of transition probabilities. (auth)

Anti-inflammatory effects of exercise

DEFF Research Database (Denmark)

Pedersen, Bente Klarlund

2017-01-01

and IL-10 is provoked by exercise and exerts direct anti-inflammatory effects by an inhibition of TNF-α and by stimulating IL-1ra, thereby limiting IL-1β signalling. Moreover, muscle-derived IL-6 appears to have direct anti-inflammatory effects and serves as a mechanism to improve glucose tolerance....... In addition, indirect anti-inflammatory effects of long-term exercise are mediated via improvements in body composition. CONCLUSION: Physical activity represents a natural, strong anti-inflammatory strategy with minor side effects and should be integrated in the management of patients with cardiometabolic...

Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa

Directory of Open Access Journals (Sweden)

Jimena Paola Angulo-Cortés

2012-04-01

Full Text Available Design of a culture media for the production of a phosphate-solubilizing bacteria co-culture. Objective. To design a complexculture media for the production of biomass and acid phosphatases from phosphate-solubilizing bacteria isolated from soil. Materialsand methods. Phosphate-solubilizing bacteria were isolated from oil palm crop soil samples and selected on SMRS1 agar, which werethen assessed with antagonism tests to verify their aptitude to form a co-culture. A Box-Behnken experimental design was applied toevaluate the effect of each one of the culture media components on the production of biomass and phosphatase enzymes at a laboratoryscale. Finally, microbial growth and enzyme production curves were carried out in order to determine their production times. Results.Five phosphate-solubilizing bacterial strains were isolated and three of them were selected based on their solubilization indices.These Gram negative strains with bacillus morphology were identified as A, B and C; their solubilization indices were 2.03, 2.12, and2.83, respectively. According to the ANOVA analyses for the Box-Behnken design, the only factor which had a significant effect onthe phosphatase activity (p<0.01 was hydrolyzed yeast, and the formulation that generated the highest biomass concentration andphosphatase activity (p<0.01 contained 10, 15 and 2.5 gL-1 of phosphoric rock, sucrose and hydrolyzed yeast, respectively. After 24hours of incubation at 100 rpm, the highest values of biomass and phosphatase activity were obtained: 11.8 logarithmic units of CFUand 12.9 phosphatase units. Conclusion. We determined that the culture media based on phosphoric rock 10 gL-1, hydrolyzed yeast 2.5gL-1 and commercial sucrose 15 gL-1 was ideal for the production of biomass and phosphatases by the strains evaluated; likewise, weproved that the hydrolyzed yeast was the only factor significantly influential for the production of phosphatases.

A rare case report of Morgagni Hernia with Organo-Axial Gastric Volvulus and concomitant Para-esophageal hernia, repaired laparoscopically in a Septuagenarian

Directory of Open Access Journals (Sweden)

Amol Mittal

Full Text Available Introduction: Simultaneous occurrence of Morgagni and the Para-esophageal hernia is a rare clinical condition with eight case reports in the English-language literature and only four managed laparoscopically. We describe a case of a Septuagenarian patient with Morgagni and concomitant Para-esophageal hernia treated laparoscopically. Presentation of a case: A 71-year-old male patient, presented with a one-month history of regurgitation of acid, retrosternal burning and vomiting after eating. Computed tomography (CT imaging demonstrated a large anterior diaphragmatic hernia, with herniation of bowel loops and anterosuperior displacement of the gastric antrum along with a grade III Para-esophageal hernia. The patient underwent simultaneous laparoscopic repair of Morgagni and Para-esophageal hernia with mesh reinforcement with Nissen’s total anti-reflux fundoplication. The patient’s postoperative recovery was uneventful. Discussion: A Morgagni Hernia is a rare congenital condition consisting of a Subcosto-sternal defect in the diaphragm. A Para-esophageal hernia is a rare variant of a hiatus hernia. Morgagni and Para-esophageal hernia may present with gastric volvulus or incarceration, requiring emergency treatment. Minimally invasive surgery is the preferred treatment, particularly for elderly patients and patients with comorbidities. The laparoscopic operation can provide excellent exposure and repair the hernia defect easily with minimal invasiveness and fewer complications. Conclusion: This case report highlights the co-existence of Morgagni and Para-esophageal hernias and validates the feasibility of laparoscopic repair of both hernias simultaneously. Keywords: Diaphragmatic hernia, Morgagni, Para-esophageal hernia, Minimally invasive surgery, Fundoplication, Case report

Aconselhamento em HIV/AIDS: pressupostos teóricos para uma prática clínica fundamentada

Directory of Open Access Journals (Sweden)

Clarisse Sampaio Pequeno

2013-06-01

Full Text Available Com o avanço da AIDS no Brasil, o Ministério da Saúde sentiu a necessidade de sensibilizar e treinar profissionais de saúde para trabalhar com questões provocadas pela infecção ou pela doença. Neste sentido, surgiu a implantação de centros de orientação e apoio sorológico (CTA, que passaram a ser a principal referência em aconselhamento. O aconselhamento em HIV/AIDS é um dispositivo utilizado por profissionais de saúde buscando trabalhar informação, orientação, avaliação de risco e apoio na realização da sorologia anti-HIV. Entretanto, faz-se necessário que esta estratégia tenha fundamentos teórico-filosóficos que sirvam para embasar a prática clínica do aconselhador. Logo, este artigo objetivou apresentar os principais pressupostos de algumas teorias do aconselhamento psicológico e sua relação com a prática do aconselhamento em HIV/AIDS. Acredita-se que estas reflexões poderão contribuir para fundamentar teoricamente os profissionais que realizam aconselhamento em HIV/AIDS, configurando-o realmente em um dispositivo para promoção da saúde.

Measurement of (anti)deuteron and (anti)proton production in DIS at HERA

International Nuclear Information System (INIS)

Chekanov, S.; Derrick, M.; Magill, S.

2007-05-01

The first observation of (anti)deuterons in deep inelastic scattering at HERA has been made with the ZEUS detector at a centre-of-mass energy of 300-318 GeV using an integrated luminosity of 120 pb -1 . The measurement was performed in the central rapidity region for transverse momentum per unit of mass in the range 0.3 T /M<0.7. The particle rates have been extracted and interpreted in terms of the coalescence model. The (anti)deuteron production yield is smaller than the (anti)proton yield by approximately three orders of magnitude, consistent with the world measurements. (orig.)

Therapeutic effects of date fruits (Phoenix dactylifera) in the prevention of diseases via modulation of anti-inflammatory, anti-oxidant and anti-tumour activity.

Science.gov (United States)

Rahmani, Arshad H; Aly, Salah M; Ali, Habeeb; Babiker, Ali Y; Srikar, Sauda; Khan, Amjad A

2014-01-01

The current mode of treatment of various diseases based on synthetic drugs is expensive, alters genetic and metabolic pathways and also shows adverse side effects. Thus, safe and effective approach is needed to prevent the diseases development and progression. In this vista, Natural products are good remedy in the treatment/management of diseases and they are affordable and effective without any adverse effects. Dates are main fruit in the Arabian Peninsula and are considered to be one of the most significant commercial crops and also have been documented in Holy Quran and modern scientific literatures. Earlier studies have shown that constituents of dates act as potent antioxidant, anti-tumour as well as anti-inflammatory, provide a suitable alternative therapy in various diseases cure. In this review, dates fruits has medicinal value are summarized in terms of therapeutic implications in the diseases control through anti-oxidant, anti-inflammatory, anti-tumour and ant-diabetic effect.

Effects of gamma radiation immunogenicity of ribonucleoprotein (RNPs) of rabies virus and purification of anti-RNPs antibodies for diagnosis; Efeitos da radiacao gama na imunogenicidade das ribonucleoproteinas (RNPs) do virus da raiva e purificacao de anticorpos anti-RNPs para diagnostico

Energy Technology Data Exchange (ETDEWEB)

Costa, Ana Elena Boamorte da

2010-07-01

The World Health Organization recommends the direct immunofluorescence test for laboratory diagnosis and serological evaluation of rabies. To achieve this test, fluorescent anti-ribo nucleoproteins (RNPs) conjugates, produced from purified IgGs of RNP-immunized animals are employed. The aims of the present study were: investigate the effects of gamma radiation on the immunogenicity of RNPs, as well as to compare two chromatographic methodologies for the purification of anti-RNPs immunoglobulins. Sera from animals immunized with either native or irradiated RNPs were compared by direct immunofluorescence and immuno enzymatic assays. Our results indicate that the animals immunized with irradiated antigen requested a lower number of doses to reach high antibody titers. The immunofluorescence assays indicated that the conjugates produced with the anti-irradiated RNPs IgGs showed similar specificity to its anti-native counterpart, but with a higher definition of the virus inclusions. The purification methods were compared by Bradford and electrophoresis assays. According to the results, we concluded that the affinity-based process resulted in higher yields, lower execution time, and higher purity of the antibodies. (author)

Extratos biotransformados de fenólicos de cítricos e atividade anti-inflamatória "in vitro"

OpenAIRE

Tais Moala

2016-01-01

Resumo: O processo inflamatório é um mecanismo de defesa desenvolvido pelo organismo com o objetivo de combater infecções (originadas por vírus, bactérias ou outros patógenos) e também injúrias (cortes ou queimaduras). Os compostos fenólicos são amplamente distribuídos em muitos produtos derivados de plantas e apresentam uma gama de propriedades benéficas para a saúde humana, como atividade antioxidante e anti-inflamatória. A atividade antioxidante desses compostos está relacionada com a pres...

Seroprevalences of anti-Sarcocystis neurona and anti-Neospora hughesi antibodies among healthy equids in the United States.

Science.gov (United States)

James, Kaitlyn E; Smith, Woutrina A; Conrad, Patricia A; Packham, Andrea E; Guerrero, Leopoldo; Ng, Mitchell; Pusterla, Nicola

2017-06-01

OBJECTIVE To describe the general seroprevalence of anti-Sarcocystis neurona and anti-Neospora hughesi antibodies among healthy equids by use of indirect fluorescent antibody tests and determine potential risk factors for seropositivity. DESIGN Cross-sectional study. SAMPLE Whole blood samples collected from 5,250 equids (1 sample/animal) across 18 states in the United States during October 2013. PROCEDURES Information regarding potential risk factors (geographic region, breed, primary use, sex, and age) was collected along with the blood samples. For each equid, an indirect fluorescent antibody test was used to determine serum titers of antibody against each of the 2 protozoal parasites. Mixed-effects logistic regression models were created to determine ORs for seropositivity. RESULTS The overall seroprevalence of anti-S neurona and anti-N hughesi antibodies in the tested equids was 78% and 34%, respectively. Of the equids, 31% were seropositive and 18% were seronegative for antibodies against both parasites. Factors associated with equids being seropositive for anti-S neurona antibodies were residence in the South, warmblood breed, and age > 5 years. Seroprevalence of anti-N hughesi antibodies did not differ among equids in different states across the country, but warmblood breed and age > 5 years were associated with seropositivity. CONCLUSIONS AND CLINICAL RELEVANCE With regard to risk factors for S neurona and N hughesi exposure and antibody response among tested equids, older age was not unexpected; however, the influences of warmblood breed and geographic location on seropositivity for anti-S neurona antibody but not for anti-N hughesi antibody deserve further investigation.

Estudio de una tecnología de extracción y faccionamiento de aceite de ñandú de alta calidad para su utilización en la industria cosmética y farmacéutica

Directory of Open Access Journals (Sweden)

Rosa Márquez

2011-05-01

Full Text Available El propósito de este estudio fue aplicar la metodología de extracción con calor en condiciones de vacío a  grasa cavitaria y exterior para la obtención de aceite de ñandú (Rhea americana con condiciones, una vez refinado,  de humedad, acidez, índice de peróxidos, color y perfil de ácidos grasos compatibles con las exigencias del comercio internacional para los aceites de ratites. En cuanto al método de refinación seleccionado fue el método de refinación alcalina. Las condiciones elegidas para la neutralización fueron: NaOH 10% y 0.1% de exceso de la misma. Luego el aceite se lava hasta un contenido de jabones inferior a 50 ppm debido a que los mismos afectan el valor de acidez. De esta forma se lograron valores de acidez muy por debajo del límite requerido por la AEA (American Emu Association para el aceite de emú extra refinado. Se utilizó tierra de blanqueo Supreme 126 (marca Tonsil de Quimica Sumex S.A., en una concentración del 0.5 %. Se determinó que no existen diferencias sustanciales entre la grasa cavitaria y exterior y en el proceso de winterización se obtienen  dos  fracciones con  perfil de ácidos grasos diferentes siendo la oleína más rica en ácidos grasos insaturados. El contenido de oleico prácticamente no varía entre las fracciones, mientras que el porcentaje de linoleico (ácido graso esencial es superior para la oleína. En el presente  trabajo se obtuvo un aceite cuyas características y parámetros de calidad son aceptables para los requerimientos internacionales que deben poseer los aceites para aplicación en cosmética, haciéndolo factible de comercializar. Por tanto los métodos de extracción y refinación adaptados para esta aplicación son adecuados y simples de aplicar.AbstractThis study was made to apply the extraction method with temperature and vaccum application to internal and external fat of ñandú (Rhea Americana to obtain a ñandú oil which once refined, has the humidity

Direct anti-atherosclerotic therapy; development of natural anti-atherosclerotic drugs preventing cellular cholesterol retention.

Science.gov (United States)

Orekhov, Alexander N

2013-01-01

The results of numerous clinical trials with statins and other drugs have demonstrated the principal possibility of the prevention and regression of atherosclerosis by pharmacotherapy. This review describes the use of cultured human arterial cells for the mass screening of anti-atherosclerotic substances, the investigation of the mechanisms responsible for their atherosclerosis-related effects, and the optimization of anti-atherosclerotic and anti-atherogenic drug and dietary therapies. Natural products can be considered promising drugs for anti-atherosclerotic therapy. Our basic studies have shown that cellular lipidosis is the principal event in the genesis of atherosclerotic lesions. Using cellular models and natural products, we have developed an approach to prevent lipid accumulation in arterial cells. Based on our knowledge of atherosclerosis, we developed drugs that possess direct anti-atherosclerotic activity. Two-year treatment with allicor (garlic powder) has a direct anti-atherosclerotic effect on carotid atherosclerosis in asymptomatic men. Inflaminat (calendula, elder, and violet), which possesses anti-cytokine activity, has been shown to cause the regression of carotid atherosclerosis following the treatment of asymptomatic men for one year. The phytoestrogen-rich drug karinat (garlic powder, extract of grape seeds, green tea leaves, hop cones, β-carotene, α-tocopherol, and ascorbic acid) prevents the development of carotid atherosclerosis in postmenopausal women. Thus, our basic findings were successfully translated into clinical practice. Because of this translation, a novel approach to antiatherosclerotic therapy was developed. Our clinical trial confirmed the efficacy of both the novel approach and the novel drugs.

Anti-biofilm and anti-adherence activities of sub fraction 18 of Melastoma malabathricum towards Streptococcus mutans

Science.gov (United States)

Rohazila M., H.; Nazlina, I.; Yaacob W., A.

2014-09-01

A study was carried out to isolate and identify the active compounds from Melastoma malabathricum stem bark that exhibit anti-biofilm and anti-adherence activities against Streptococcus mutans. Purification of the active compounds from the stem bark extract was performed via silica gel chromatography to produce 12 fractions. Further fractionation of fraction 9 by high performance liquid chromatography (HPLC) produced 21 sub fractions. All the sub fractions were subjected to thin layer chromatography (TLC) bioautography as preliminary screening to determine anti bacterial activity. TLC-bioautography showed that sub fraction 18 (SF18) demonstrated large inhibited zone against S. mutans. Gas chromatography mass spectrometry (GCMS) was used to identify the active compounds in SF18. Fraction SF18 revealed 27 compounds such as hexanoic acid, 8-methyl-1-undecene, propanenitrile, and 1-decene. Anti-biofilm and anti-adherence activities were determined using crystal violet and glass surface assays respectively. The concentrations that produced 50% reduction in anti-biofilm and anti-adherence activities were 1.88 mg/ml and 3.75 mg/ml respectively.

GC-MS analysis, evaluation of phytochemicals, anti-oxidant, thrombolytic and anti-inflammatory activities of Exacum bicolor

Directory of Open Access Journals (Sweden)

Appaji Mahesh Ashwini

2015-12-01

Full Text Available The aim of the present study was to investigate the GC-MS analysis, phytochemical screening, anti-oxidant, thrombolytic and anti-inflammatory activities of methanol extract of leaves of Exacum bicolor. FTIR analysis confirmed the presence of alcohol, phenols, alkanes, aromatic compounds, aldehyde and ethers. GC-MS analysis revealed the presence of eight phyto-constituents. The total phenol, flavonoid and alkaloid contents were 18.0 ± 0.2 mg/GAE/g, 13.1 ± 0.4 mg QE/g and 108.0 ± 1.2 mg AE/g respectively. The DPPH assay exhibited potent anti-oxidant abilities with IC50 8.8 µg/mL. Significant thrombolytic activity was demonstrated by clot lysis method (45.1 ± 0.8%. The methanol extract showed significant membrane stabilization on human red blood cell with IC50 value of 37.4 µg/mL. There was a significant correlation (R2>0.98 with total phenolic content versus anti-oxidant and anti-inflammatory activity. The above results confirmed that E. bicolor could be a promising anti-oxidant, thrombolytic and anti-inflammatory agent.

EU Lobbying and Anti-Dumping Policy

DEFF Research Database (Denmark)

Nielsen, Jørgen Ulff-Møller; Svendsen, Gert Tinggaard

2012-01-01

of petitioning firms and Council voting in the case of anti-dumping policy. If the political position of countries in anti-dumping cases is influenced by domestic lobbying efforts, we expect that the empirical pattern of country distribution of petitioning firms in EU anti-dumping cases corresponds closely...... to the empirical pattern of EU country distribution in Council voting. Our results show a low petitioning intensity for anti-dumping investigations and a high voting intensity against anti-dumping measures in Northern Europe. Thus, it seems likely that domestic lobbying efforts have influenced the political...

Contribuição para a padronização química de Hancornia speciosa Gomes: desenvolvimento e validação de métodos analíticos para a quantificação de marcadores químicos

OpenAIRE

Ana Barbara Dias Pereira

2012-01-01

Hancornia speciosa Gomes, popularmente conhecida como mangaba, tem uso etnomédico para o tratamento de hipertensão e diabetes, entre outros. A potencial atividade anti-hipertensiva da espécie foi relatada em trabalhos anteriores de nosso grupo em modelos in vitro, ex vivo e in vivo, sendo rutina, L-(+)-bornesitol e ácido quínico identificados como constituintes bioativos. O presente trabalho teve como objetivo uantificar os principais constituintes químicos em extratos de folhas da espécie, v...

Online Anti-Brand Herds

DEFF Research Database (Denmark)

Langley, David J.; Tan, Chee-Wee; Worm, Daniël

The online environment offers a fertile breeding ground for anti-brand herds of disgruntled consumers. Firms are often caught off guard by the unpredictability of such herds and, as a consequence, are forced into a reactive, defensive stance. We conduct a social media analysis that aims to shed...... light on the formation, growth, and dissolution of online anti-brand herds. First we expand on the concept of environmental turbulence to advance core properties unique to online herd behavior. Next, based on evidence gathered from 40 online anti-brand herd episodes targeting two prominent firms from...... the Netherlands, we develop an analytical model to investigate drivers of herd formation, growth, and dissolution. Finally, combining environmental turbulence literature with our empirical findings, we derive a novel typology of online anti-brand herd behaviors, and put forward six propositions to guide theory...

Modelos para estimar consumo e exigências nutricionais para poedeiras comerciais

OpenAIRE

Palma Bendezu, Hilda Cristina [UNESP

2016-01-01

O objetivo deste trabalho foi elaborar um modelo para calcular as ingestões de aminoácidos e energia para a fase de maturação sexual e produção de ovos. As exigências de energia, aminoácidos e consumo de ração foram calculados pelo método fatorial, considerando na fase de maturação sexual a quantidade de nutrientes requerida para mantença e a quantidade de nutrientes necessários para crescimento e deposição de nutrientes corporais em cada componente do corpo (ovário, oviduto, corpo livre de ...

Anti-deuteron sensitivity studies at LHCb

CERN Multimedia

Baker, Sophie Katherine

2018-01-01

Measurements of anti-deuterons in collider experiments can help to reduce systematic uncertainties in indirect searches for dark matter. Two predominant unknowns in these searches are the production of secondary anti-deuterons in the cosmos from spallation processes, and anti-deuteron production from annihilating dark matter. LHCb is a forward spectrometer on the LHC ring, designed to measure b-hadron decays from high energy proton-proton collisions. With the detector's excellent particle identification capabilities, deuteron and anti-deuteron measurements at LHCb could help to parametrise the two cosmological processes. Recent studies of (anti-)deuteron identification at LHCb and the prospects for measuring prompt (anti-)deuterons from pp-collisions will be presented, as well as a working analysis of b-baryrons decaying to deuterons.

Uso e Percepção de Licitude de Práticas Ilegais ou Antiéticas de Sobrevivência de Micro e Pequenas Empresas

Directory of Open Access Journals (Sweden)

Sérgio Henrique Arruda Cavalcante Forte

2015-11-01

Full Text Available Segundo o Sebrae (2013, a taxa de sobrevivência das Micro e Pequenas Empresas (MPE no Brasil vem melhorando, com sobrevivência de 75,6% nos primeiros dois anos de vida. Das sobreviventes, muitas enfrentam dificuldades no mercado, tendo que por vezes optar para o uso de práticas ilícitas ou antiéticas e ainda não se considerando “fora da lei”. Nesse contexto, o objetivo geral é relacionar o uso das práticas ilegais ou antiéticas utilizadas pelas MPE para sobreviverem com a percepção de ilicitude dessas práticas. Esta pesquisa é do tipo descritiva e quantitativa. Após levantamento bibliográfico e pesquisa exploratória com 17 MPE foi realizada uma pesquisa de campo com 102 MPE, com a aplicação de um questionário com 33 variáveis de práticas ilegais ou antiéticas, onde as práticas deveriam ser avaliadas pelo respondente com base no comportamento observado de seus concorrentes, por meio de uma escala Likert, de cinco pontos. Pode-se concluir que todas as práticas investigadas estão sendo utilizadas em algum grau pelos empreendedores, cabendo destacar que para apenas quatro das 33 práticas a percepção de ilicitude se apresentou menor que a de licitude, evidenciando que a maioria (29 de 33 práticas vem sendo utilizada mesmo tendo-se a consciência de que são ilícitas. Pode-se concluir também que das nove práticas destacadas, cinco delas referem-se à evasão fiscal. Percebeu-se que as práticas que ferem diretamente o interesse do consumidor não são vistas como “ilícitas” pelos empreendedores.

Transformations in French Anti-Semitism

Directory of Open Access Journals (Sweden)

Nonna Mayer

2007-01-01

Full Text Available The increase in the number of anti-Semitic acts since the start of the Second Intifada has sparked off a broad debate on the return of anti-Semitism in France. This article focuses on the question whether this anti-Semitism is still based on the alleged superiority of the Aryan race as in the time of Nazism, or if it represents the birth of a “new Judeophobia” that is more based on anti-Zionism and the polemical mixing of “Jews,” “Israelis,” and “Zionists.” One supposed effect of this transformation is that anti-Semitism is in the process of changing camps and migrating from the extreme right to the extreme left of the political arena, to the “altermondialistes,” the communists, and the “neo-Trotskyists.”

Modelos para estimar as exigências de energia metabolizável para poedeiras

OpenAIRE

Sakomura,Nilva Kazue; Basaglia,Roberta; Sá-Fortes,Cristina M. L.; Fernandes,João Batista K.

2005-01-01

Objetivou-se, com este trabalho, elaborar um modelo para estimar as exigências de energia metabolizável (EM) para poedeiras leves da linhagem Lohmann LSL, utilizando-se o método fatorial. Para determinar o efeito da temperatura sobre as exigências de EM para mantença, foram conduzidos experimentos em câmaras climáticas com temperaturas constantes de 12, 22 e 31ºC, utilizando a técnica do abate comparativo. A exigência de energia líquida para o ganho de peso foi determinada por meio da regress...

Production of double antibody for radioimmunoassay (sheep anti-rabbit IgG antiserum); Producao de duplo anticorpo para radioimunoensaio (antissoro de carneiro anti-IgG de coelho)

Energy Technology Data Exchange (ETDEWEB)

Silva, S.R. da

1994-12-31

A second antibody (sheep anti-rabbit IgG antiserum) to be used in RIAs in which the first antibody is raised in rabbits was produced. For this production, initially the IgG was isolated from rabbit serum and purified by sodium sulphate precipitation followed by ion exchange chromatography on DEAE-cellulose. Four sheep were immunized with 500 u g of purified rabbit IgG, emulsified in Freund Complete Adjuvant and administered by multisite subcutaneous injections. These injections were repeated at 20-days intervals and blood samples (40 ml) were taken from the jugular vein 10 days after the boosts for the evaluation of the antisera title. After each four boosts a great bleeding was done by the same route. Approximately 500 ml of serum were obtained in each bleeding per animal. The antisera were evaluated by the human thyrotropin RIA developed at IPEN laboratories employing reagents provided by NIDDKD, USA. These evaluations referred to the determination of the antisera title and of the ideal concentration of carrier IgG, to the study of the kinetic of precipitation and to the confirmation of the inexistent cross-reactivity with human IgG, in comparison with a reference antiserum of know precipitation characteristics supplied by the Radioassay System Laboratories. Approximately 3,6 l of antiserum (sheep anti-rabbit IgG serum) were produced from the four sheep, which presented title and precipitation characteristics very similar to those exhibited by the imported commercial product, even presenting higher titles. The results obtained in this work indicated that it was created enough experience for the production of this biological reagent for RIA, that could be done integrally in the country in greater scale, and at a very reduced cost. (author). 81 refs, 36 figs, 33 tabs.

Comparação dos anticorpos anti-reticulina e antiendomísio classe IGA para diagnóstico e controle da dieta na doença celíaca Comparison of IgA class reticulin and endomysium antibodies for diagnosis and control of the diet in celiac disease

Directory of Open Access Journals (Sweden)

Lorete Maria da Silva KOTZE

1999-12-01

Full Text Available Sensibilidade ao glúten é um estado de elevada resposta iamunológica (celular e humoral à ingestão de proteínas do glúten do trigo, centeio, cevada e aveia, em indivíduos geneticamente predispostos. A doença celíaca é sua expressão mais freqüente, variando as formas de apresentação. Tem como tratamento a exclusão de alimentos contendo as gliadinas tóxicas. Embora a biopsia do intestino delgado proximal seja necessária, tem-se ressaltado a importância de testes sorológicos no rastreamento, diagnóstico e monitorização da dieta isenta de glúten em pacientes com doença celíaca. O objetivo do presente estudo foi investigar a presença dos anticorpos antiendomísio (EmA-IgA e anti-reticulina (ARA-IgA em 56 pacientes celíacos (17 recém diagnosticados; 24 aderentes à dieta; 15 com transgressão à dieta. Os anticorpos foram detectados por imunofluorescência indireta, utilizando como substrato cordão umbilical humano para os EmA-IgA, fígado e rim de rato para os ARA-IgA. Nos pacientes recém diagnosticados e no grupo com transgressão à dieta houve positividade total de 100% para os EmA-IgA e 59,4% para ARA-IgA. Nos pacientes aderentes à dieta nenhum dos anticorpos foi detectado. Dentre os 32 pacientes positivos, a concordância foi de 59,4% (19, sendo que 40,6 % (13/32 eram ARA-IgA negativo e EmA-IgA positivo. Nenhum paciente mostrou-se positivo para os ARA-IgA e negativo para os EmA-IgA. Portanto, a sensibilidade para os EmA-IgA foi de 100% e de 59,4% para os ARA-IgA. A associação dos dois testes não aumentou os índices de positividade total nas amostras. Conclui-se que, atualmente, a pesquisa dos EmA-IgA pode constituir teste sorológico de escolha, seja para diagnóstico, seja para seguimento dos pacientes celíacos, pelo alto valor preditivo, alta sensibilidade e especificidade e relativo baixo custo quando se utiliza cordão umbilical humano como substrato.Sensibility to gluten is a condition with high

Consumption of high-dose vitamin C (1250 mg per day) enhances functional and structural properties of serum lipoprotein to improve anti-oxidant, anti-atherosclerotic, and anti-aging effects via regulation of anti-inflammatory microRNA.

Science.gov (United States)

Kim, Seong-Min; Lim, So-Mang; Yoo, Jeong-Ah; Woo, Moon-Jea; Cho, Kyung-Hyun

2015-11-01

Background Although the health effects of vitamin C are well known, its physiological effect on serum lipoproteins and microRNA still remain to be investigated, especially daily consumption of a high dosage. Objectives To investigate the physiological effect of vitamin C on serum lipoprotein metabolism in terms of its anti-oxidant and anti-glycation activities, and gene expression via microRNA regulation. Methods We analyzed blood parameters and lipoprotein parameters in young subjects (n = 46, 22 ± 2 years old) including smokers who consumed a high dose of vitamin C (1250 mg) daily for 8 weeks. Results Antioxidant activity of serum was enhanced with the elevation of Vit C content in plasma during 8 weeks consumption. In the LDL fraction, the apo-B48 band disappeared at 8 weeks post-consumption in all subjects. In the HDL fraction, apoA-I expression was enhanced by 20% at 8 weeks, especially in male smokers. In the lipoprotein fraction, all subjects showed significantly reduced contents of advanced glycated end products and reactive oxygen species (ROS). Triglyceride (TG) contents in each LDL and HDL fraction were significantly reduced in all groups following the Vit C consumption, suggesting that the lipoprotein was changed to be more anti-inflammatory and atherogenic properties. Phagocytosis of LDL, which was purified from each individual, into macrophages was significantly reduced at 8-weeks post-consumption of vitamin C. Anti-inflammatory and anti-senescence effects of HDL from all subjects were enhanced after the 8-weeks consumption. The expression level of microRNA 155 in HDL3 was reduced by 49% and 75% in non-smokers and smokers, respectively. Conclusion The daily consumption of a high dose of vitamin C for 8 weeks resulted in enhanced anti-senescence and anti-atherosclerotic effects via an improvement of lipoprotein parameters and microRNA expression through anti-oxidation and anti-glycation, especially in smokers.

¿Buenas razones para quemar libros? Un estudio exploratorio sobre la quema de bibliotecas barriales en Francia

Directory of Open Access Journals (Sweden)

Denis Merklen

2010-07-01

Full Text Available El trabajo se concentra en la conflictividad social de jovenes de sectores populares en las afueras de París. El texto analiza la quema de bibliotecas en los suburbios parisinos tratando de aportar claves para la comprensión de los hechos. Tratando de iluminar, por un lado, por qué un disturbio anti-policial tiene como blanco las bibliotecas populares y por otro, como esas acciones constituyen un choque político cultural de diferentes sectores sociales, alrededor de los diferentes usos de bienes culturales.

Measurement of (anti)deuteron and (anti)proton production in DIS at HERA

Energy Technology Data Exchange (ETDEWEB)

Chekanov, S.; Derrick, M.; Magill, S. [Argonne National Laboratory, Argonne, IL (US)] (and others)

2007-05-15

The first observation of (anti)deuterons in deep inelastic scattering at HERA has been made with the ZEUS detector at a centre-of-mass energy of 300-318 GeV using an integrated luminosity of 120 pb{sup -1}. The measurement was performed in the central rapidity region for transverse momentum per unit of mass in the range 0.3anti)deuteron production yield is smaller than the (anti)proton yield by approximately three orders of magnitude, consistent with the world measurements. (orig.)

Sero-Prevalence of anti-sperm anti-bodies in infertile males in Port ...

African Journals Online (AJOL)

Background: Infertility is a serious health challenge which causes distress to the couples especially; in Africa. The cause of infertility is multifactorial. Immunological infertility is said to be one of the major causes of unexplained infertility in men. Anti-sperm anti-bodies can be used as an immunological marker of infertility.

Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica Marcadores inflamatorios y anticuerpos anti-chlamydia en pacientes con síndrome metabólico Inflammatory markers and antichlamydial antibodies in patients with metabolic syndrome

Directory of Open Access Journals (Sweden)

Rosecler Riethmuller Franco

2011-02-01

Full Text Available FUNDAMENTO: A síndrome metabólica está associada ao aumento de risco de eventos cardiovasculares. Marcadores inflamatórios e anticorpos anti-Chlamydia têm sido relacionados ao desenvolvimento e à progressão da aterosclerose e dos eventos cardiovasculares. OBJETIVO: Avaliar os marcadores inflamatórios interleucina-6 (IL-6 e fator de necrose tumoral-alfa (TNF-α e os anticorpos anti-Chlamydia pneumoniae em pacientes com síndrome metabólica (SM, com e sem eventos cardiovasculares. MÉTODOS: Estudo transversal constituído por 147 indivíduos. Desses, 100 (68% com SM e sem eventos cardiovasculares; e 47 (32% com SM e com eventos cardiovasculares. Dos indivíduos que sofreram eventos cardiovasculares, 13 (6,11% apresentam infarto agudo do miocárdio (IAM, e dez (4,7%, acidente vascular cerebral (AVC. O diagnóstico da SM foi determinado pelos critérios do NCEP-ATPIII. RESULTADOS: A média de idade dos sujeitos com eventos cardiovasculares foi de 61,26 ± 8,5 e de 59,32 ± 9,9 nos indivíduos sem esses eventos (p=0,279, havendo predomínio do sexo feminino. O grupo com SM e sem evento apresentou maior peso, altura, IMC e circunferência abdominal. Para os indivíduos com eventos cardiovasculares (p=0,001, os marcadores inflamatórios IL-6 e TNF-α e a doença vascular periférica foram significativamente maiores. Obtiveram-se níveis elevados de anticorpos IgG para Chlamydia pneumoniae no grupo SM, sem eventos e de IgA no grupo com eventos quando comparados os dois grupos. Com relação ao IAM e ao AVC, os anticorpos anti-Chlamydia pneumoniae não demonstraram significância estatística, comparados ao grupo sem eventos cardiovasculares. Associação foi observada com o uso de estatinas, hipoglicemiantes orais, injetáveis e anti-inflamatórios não esteroidais no grupo com esses eventos. CONCLUSÃO: Marcadores inflamatórios encontram-se significativamente elevados em pacientes com SM, com IAM e AVC. Anticorpos anti-Chlamydia n

Relationship between serum concetrations of type III procollagen, hyluronic acid and histopathological findings in the liver of HCV-positive blood donors Relação entre concentrações séricas de procolágeno tipo III, ácido hialurônico com achados histopatológicos do fígado em doadores de sangue anti-HCV positivos

Directory of Open Access Journals (Sweden)

Vera Regina Rodrigues Camacho

2007-06-01

Full Text Available BACKGROUND: Serologic markers have been proposed for monitoring hepatic fibrosis in chronic liver disease. Among fibrosis markers, type III procollagen (PIIIP and hyaluronic acid have been studied in these patients. AIM: To evaluate the association between these serum markers with histological findings. METHODS: A prospective cross-sectional study was carried out with HCV-positive blood donors. The studied population included men and women whose age ranged from 18 to 60 years, with elevated liver function tests [ALT levels > 1.5 times the normal value and alterations of two or more of the following: any changes in the levels of ALT, aspartate aminotransferase, conjugated bilirrubin, gammaglobulin, gammaglutamyltranspeptidase, albumin, platelet count; alkaline phosphatase levels >1.5 times the normal value, or prothrombin time below 70% and above 60%]. Fourty-nine patients were submitted to liver biopsy, blood analysis of PIIIP, hyaluronic acid, besides liver function tests. RESULTS: Liver function tests were not associated with tissular fibrosis, as assessed by ALT (>1.5 times above normal, fibrosis risk=18.8%; RACIONAL: Marcadores sorológicos têm sido propostos para monitorar fibrose hepática em doença crônica do fígado. Dentre os marcadores de fibrose, ácido hialurônico e procolágeno tipo III têm sido estudados nestes pacientes. OBJETIVO: Avaliar a associação de marcadores séricos de fibrose com achados histológicos. MÉTODOS: Foi realizado estudo transversal prospectivo em doadores de sangue anti-HCV positivos. A população estudada incluiu homens e mulheres com idade entre 18-60 anos com provas de função hepática alteradas (níveis de alanina aminotransferase >1.5 vezes do normal e alterações de dois ou mais dos seguintes: qualquer alteração nos níveis de alanina aminotransferase, aspartato aminotransferase, bilirrubina conjugada, gamaglobulina, gamaglutamiltranspeptidase, albumina, plaquetas, níveis de fosfatase

Anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica.

Science.gov (United States)

Mohan, C G; Deepak, M; Viswanatha, G L; Savinay, G; Hanumantharaju, V; Rajendra, C E; Halemani, Praveen D

2013-04-13

To evaluate the anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions. In vitro DPPH radical scavenging activity and lipoxygenase (LOX) inhibition assays were used to evaluate the anti-oxidant and anti-inflammatory activities respectively. Methanolic extract (MEMI), successive water extract (SWMI) and ethyl acetate fraction (EMEMI), n-butanol fraction (BMEMI) and water soluble fraction (WMEMI) of methanolic extract were evaluated along with respective reference standards. In in vitro DPPH radical scavenging activity, the MEMI, EMEMI and BMEMI have offered significant antioxidant activity with IC(50) values of 13.37, 3.55 and 14.19 μg/mL respectively. Gallic acid, a reference standard showed significant antioxidant activity with IC(50) value of 1.88 and found to be more potent compared to all the extracts and fractions. In in vitro LOX inhibition assay, the MEMI, EMEMI and BMEMI have showed significant inhibition of LOX enzyme activity with IC(50) values of 96.71, 63.21 and 107.44 μg/mL respectively. While, reference drug Indomethacin also offered significant inhibition against LOX enzyme activity with IC(50) of 57.75. Furthermore, MEMI was found to more potent than SWMI and among the fractions EMEMI was found to possess more potent antioxidant and anti-inflammatory activity. These findings suggest that the MEMI and EMEMI possess potent anti-oxidant and anti-inflammatory activities in in vitro conditions. Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

Differential requirement for nitric oxide in IGF-1-induced anti-apoptotic, anti-oxidant and anti-atherosclerotic effects

Science.gov (United States)

Sukhanov, Sergiy; Higashi, Yusuke; Shai, Shaw-Yung; Blackstock, Christopher; Galvez, Sarah; Vaughn, Charlotte; Titterington, Jane; Delafontaine, Patrick

2011-01-01

We have shown previously that insulin like-growth factor I (IGF-1) suppressed atherosclerosis in Apoe−/− mice and activated endothelial nitric oxide (NO) synthase. To determine whether IGF-1-induced atheroprotection depends on NO, IGF-1- or saline-infused mice were treated with L-NAME, the pan-NO synthase inhibitor or with D-NAME (control). IGF-1 reduced atherosclerosis in both the D-NAME and L-NAME groups suggesting that IGF-1’s anti-atherogenic effect was NO-independent. IGF-1 increased plaque smooth muscle cells, suppressed cell apoptosis and downregulated lipoprotein lipase and these effects were also NO-independent. On the contrary, IGF-1 decreased oxidative stress and suppressed TNF-α levels and these effects were blocked by L-NAME. Thus IGF-1’s anti-oxidant effect is dependent on its ability to increase NO but is distinct from its anti-atherosclerotic effect which is NO-independent. PMID:21872589

Anti-inflammatory and anti-oxidative effects of herbal preparation EM 1201 in adjuvant arthritic rats

Directory of Open Access Journals (Sweden)

Laimis Akramas

2015-01-01

Conclusions: The present study suggests that EM 1201 has protective activity against arthritis and demonstrated its potential beneficiary effect analogical to diclofenac. Anti-inflammatory and anti-oxidative effect of EM 1201 in rats with AA support the need of further investigations by using it as supplementary agent alone or together with other anti-arthritic drugs in the treatment of rheumatoid arthritis.

Lectins with Anti-HIV Activity: A Review

Directory of Open Access Journals (Sweden)

Ouafae Akkouh

2015-01-01

Full Text Available Lectins including flowering plant lectins, algal lectins, cyanobacterial lectins, actinomycete lectin, worm lectins, and the nonpeptidic lectin mimics pradimicins and benanomicins, exhibit anti-HIV activity. The anti-HIV plant lectins include Artocarpus heterophyllus (jacalin lectin, concanavalin A, Galanthus nivalis (snowdrop agglutinin-related lectins, Musa acuminata (banana lectin, Myrianthus holstii lectin, Narcissus pseudonarcissus lectin, and Urtica diocia agglutinin. The anti-HIV algal lectins comprise Boodlea coacta lectin, Griffithsin, Oscillatoria agardhii agglutinin. The anti-HIV cyanobacterial lectins are cyanovirin-N, scytovirin, Microcystis viridis lectin, and microvirin. Actinohivin is an anti-HIV actinomycete lectin. The anti-HIV worm lectins include Chaetopterus variopedatus polychaete marine worm lectin, Serpula vermicularis sea worm lectin, and C-type lectin Mermaid from nematode (Laxus oneistus. The anti-HIV nonpeptidic lectin mimics comprise pradimicins and benanomicins. Their anti-HIV mechanisms are discussed.

Anti-synchronization of chaotic oscillators

International Nuclear Information System (INIS)

Kim, Chil-Min; Rim, Sunghwan; Kye, Won-Ho; Ryu, Jung-Wan; Park, Young-Jai

2003-01-01

We have observed anti-synchronization phenomena in coupled identical chaotic oscillators. Anti-synchronization can be characterized by the vanishing of the sum of relevant variables. We have qualitatively analyzed its base mechanism by using the dynamics of the difference and the sum of the relevant variables in coupled chaotic oscillators. Near the threshold of the synchronization and anti-synchronization transition, we have obtained the novel characteristic relation

Utilização, em politransfundidos, da pesquisa de anticorpos igm anti-trypanosoma cruzi e anti-toxoplasma gondii para detectar infecções pós-transfusionais recentes IgM Trypanosoma cruzi and Toxoplasma gondii antibodies in the detection of recent transfusion-transmitted infections

Directory of Open Access Journals (Sweden)

Vicente Amato Neto

1984-04-01

Full Text Available Consideram os Autores que a pesquisa de anticorpos IgM no soro é tática capaz de revelar recentes infecções pós-transfusionais. Por isso, decidiram usar esse tipo de mensuração relativamente a grupo constituído por 101 politrans-fundidos, tendo abordado especificamente as aquisições de doença de Chagas e toxoplasmose. Através da investigação que realizaram, só em duas oportunidades encontraram anticorpos IgM anti-Trypanosoma cruzi ou anti-Toxoplasma gondii e, portanto, não evidenciaram expressivo panorama tradutor de processos há pouco tempo contraídos, como ainda, por meio de anticorpos IgG não identificaram números expressivos de pessoas com essas protozooses. No entanto, detectaram a expressiva taxa de 4,9% de casos de doença de Chagas muito provavelmente decorrentes da hemoterapia. A despeito da relevância não acentuada dos resultados que obtiveram, julgaram os Autores ser válido estimular a efetivação de outros estudos congêneres e correlatos, aptos a contribuir para aqui-latamento de riscos pertinentes à prática hemoterápica.The Authors have regarded serum IgM antibodies titration as useful in the detection of recent transfusion-transmitted infections. For this reason a group consisting of 101 patients, who had received many blood transfusions, underwent such mensuration in order to reveal recent Chagas'disease and toxoplasmosis acquired infections. Throughout the investigation just two cases have yielded IgM trypanosomal or toxoplasmal antibodies, showing therefore that this sort of titration did not correlate with the real existence of recent acquired infections. On the other hand IgM antibodies in the same patients did not show a considerable incidence of these two protozoan infections. However an expressive rate of 4.9% of Chagas'disease probably due to hemotherapy was found. Although the results this study were not very relevant, the Authors still have in mind that further similar investigations should be

Protecting Anti-virus Programs From Viral Attacks

OpenAIRE

Mishra, Umakant

2013-01-01

During a fight between viruses and anti-viruses it is not always predictable that the anti-virus is going to win. There are many malicious viruses which target to attack and paralyze the anti-viruses. It is necessary for an anti-virus to detect and destroy the malware before its own files are detected and destroyed by the malware. The anti-virus may follow thorough testing and auditing procedures to fix all its bugs before releasing the software in the market. Besides the anti-virus may use a...

Significados da gravidez e da maternidade: discursos de primíparas e multíparas

OpenAIRE

Silva, Flavia Baroni; Souza, Laura Vilela e; Scorsolini-Comin, Fabio

2013-01-01

Resumo: O objetivo foi compreender os sentidos sobre maternidade e as vivências da gravidez em gestantes primíparas e multíparas. Foram entrevistadas seis gestantes, sendo três primíparas e três multíparas. Nas falas, foram trazidos temas como dificuldades físicas, alterações corporais, repercussão da gravidez no relacionamento conjugal, planejamento da gravidez e envolvimento paterno. Observou-se, nas primíparas, que a inexperiência trouxe insegurança, ao passo que, em algumas multíparas, ho...

Produção e purificação de imunoglobulinas Y policlonais anti-Leptospira spp.

Directory of Open Access Journals (Sweden)

Tatiane C.F. Tavares

2013-09-01

Full Text Available Objetivou-se verificar se galinhas imunizadas com uma solução de Leptospira interrogans inativadas e proteínas de membrana externa do sorovar Hardjo, poderiam produzir anticorpos policlonais específicos anti-leptospiras, detectáveis em testes ELISA. Foram imunizados oito galinhas com 25 semanas de idade, da raça White Leghorn, sendo três imunizadas com uma suspensão de leptospiras inativadas, três com uma solução de proteínas de membrana externa extraída do sorovar Hardjo e duas controle. Coletas de sangue foram realizadas quinzenalmente e de ovos diariamente. A IgY foi purificada a partir da gema dos ovos utilizando para a delipidação o método de diluição em água ácida e a precipitação com sulfato de amônio. Nos testes ELISA realizados para verificar a especificidade da IgY, foi demonstrada a produção de anticorpos anti-Leptospira, tanto no soro quanto nas gemas purificadas. O pico de produção de anticorpos específicos ocorreu na 5º semana após a primeira imunização. Ficou demonstrada a possibilidade da indução da produção de anticorpos específicos em galinhas imunizadas com leptospiras do sorovar Hardjo inativadas, bem como, com proteínas de membrana externa (PME extraidas desse sorovar. As galinhas imunizadas com uma suspensão de leptospiras inativadas ou com PME de Leptospira interrogans do sorovar Hardjo produziram anticorpos reativos a PME Hardjo detectáves por teste ELISA.

Polarized antiquark flavor asymmetry DELTA anti u(x)-DELTA anti d(x) and the pion cloud

CERN Document Server

Fries, R J; Weiss, C

2003-01-01

The flavor asymmetry of the unpolarized antiquark distributions in the proton, anti u(x)- anti d(x)0. Using a simple chiral linear sigma model as an example, we demonstrate that in the meson cloud picture a large positive DELTA anti u(x)-DELTA anti d(x) can be obtained from pi-sigma interference contributions. This calls into question previous estimates based on rho-meson contributions alone, and indicates how the results of the meson cloud picture may be reconciled with those of quark-based models. (orig.)

Stress proteins and auxiliary anti-stress compounds in intertidal macroalgae Proteínas de estrés y compuestos anti-estrés auxiliares en algas marinas intermareales

Directory of Open Access Journals (Sweden)

Edgardo Cruces

2012-11-01

és (HSPs han sido consideradas como potenciales agentes protectores en respuesta a condiciones estresantes, especialmente durante la acción de elevada temperatura y alta radiación solar. Considerando que las macroalgas marinas no han sido usadas comúnmente como modelos de estudio para analizar estas moléculas, el presente trabajo compila la información existente sobre la inducción de proteínas de estrés en algas intermareales en el contexto de los principales factores hasta ahora reportados como inductores, e.g. temperatura, alta radiación solar, contaminantes, etc. Adicionalmente, mediante un estudio de caso usando dos especies del intermareal superior, Ulva sp. y Porphyra columbina colectadas en el sur de Chile, se examina si la inducción de proteínas de estrés ocurre de forma complementaria con otras respuestas anti-stress. Para ello, dos mecanismos bien conocidos, fotoinhibición de fotosíntesis y actividad antioxidante, fueron medidos en paralelo. Los resultados indicaron que, aunque hubo expresión de proteínas de estrés dentro de un periodo experimental entre 3 y 24 h, estas respuestas no fueron correlacionadas con cambios en fotosíntesis o actividad antioxidante. En general, el estudio perfila un potencial rol de estas moléculas en algunas respuestas ecofisiológicas desarrolladas para contrarrestar los efectos negativos de las altas temperaturas y la radiación solar. Por otro lado, otros ajustes metabólicos de acción rápida (e.g. fotoinhibición y actividad antioxidante, distintas biomoléculas (micosporinas, fenoles, poliaminas, etc., además de las adecuaciones morfo-funcionales a la vida intermareal (e.g. pequeno tamano, alta proporción área/volumen son importantes para explicar la fisiología de estos organismos.

Estrategias para negociaciones exitosas.

OpenAIRE

José B. Parra V; Evelinda Santiago J.; Misael Murillo M.; Candy Atonal N.

2010-01-01

Las estrategias de negociación son herramientas para disminuir conflictos o para intercambiar bienes y servicios. Una negociación exitosa crea relaciones de solidaridad y propicia reciprocidad, cooperación y confianza para quienes tendrán relaciones permanentes de negociación con beneficios para ambas partes. Una estrategia de negociación exitosa requiere manejar el desarrollo del ambiente social del grupo de personas, con la finalidad de tener presente las fronteras que no pueden irrumpirse....

In vitro anti-thrombotic and anti-coagulant properties of blacklip abalone (Haliotis rubra) viscera hydrolysate.

Science.gov (United States)

Suleria, Hafiz Ansar Rasul; Masci, Paul P; Addepalli, Rama; Chen, Wei; Gobe, Glenda C; Osborne, Simone A

2017-07-01

Abalone viscera contain sulphated polysaccharides with anti-thrombotic and anti-coagulant activities. In this study, a hydrolysate was prepared from blacklip abalone (Haliotis rubra) viscera using papain and bromelain and fractionated using ion exchange and size exclusion chromatography. Hydrolysates and fractions were investigated for in vitro thrombin inhibition mediated through heparin cofactor II (HCII) as well as anti-coagulant activity in plasma and whole blood. On the basis of sulphated polysaccharide concentration, the hydrolysate inhibited thrombin through HCII with an inhibitor concentration at 50% (IC50) of 16.5 μg/mL compared with 2.1 μg/mL for standard heparin. Fractionation concentrated HCII-mediated thrombin inhibition down to an IC50 of 1.8 μg/mL and improved anti-coagulant activities by significantly delaying clotting time. This study confirmed the presence of anti-thrombotic and anti-coagulant molecules in blacklip abalone viscera and demonstrated that these activities can be enriched with a simple chromatography regime. Blacklip abalone viscera warrant further investigation as a source of nutraceutical or functional food ingredients. Graphical abstract Schematic showing preparation of bioactive extracts and fractions from blacklip abalone.

Discrete anti-gravity

International Nuclear Information System (INIS)

Noyes, H.P.; Starson, S.

1991-03-01

Discrete physics, because it replaces time evolution generated by the energy operator with a global bit-string generator (program universe) and replaces ''fields'' with the relativistic Wheeler-Feynman ''action at a distance,'' allows the consistent formulation of the concept of signed gravitational charge for massive particles. The resulting prediction made by this version of the theory is that free anti-particles near the surface of the earth will ''fall'' up with the same acceleration that the corresponding particles fall down. So far as we can see, no current experimental information is in conflict with this prediction of our theory. The experiment crusis will be one of the anti-proton or anti-hydrogen experiments at CERN. Our prediction should be much easier to test than the small effects which those experiments are currently designed to detect or bound. 23 refs

PERILAKU ANTI SOSIAL PADA ANAK SEKLOAH DASAR

Directory of Open Access Journals (Sweden)

Ratna Sari Dewi

2015-09-01

Full Text Available Perilaku Anti Sosial merupakan perilaku negatif atau perilaku yang menyimpang dari norma-norma, baik aturan keluarga, sekolah, masyarakat, maupun hukum. Jenis perilaku anti sosial pada anak sekolah dasar diantaranya perilaku negativisme, agresi dan tingkah laku menguasai. Faktor risiko yang menyebabkan perilaku anti sosial pada anak-anak dapat dikategorikan sebagai faktor pribadi (personal risk factors, keluarga (family risk factors, berkaitan dengan sekolah (school-related risk factors dan sosial (social risk factors. Upaya penanganan anak dengan anti sosial dapat dilakukan dengan upaya orang tua menerapkan pola asuh authoritative. Jika terlanjur berperilaku anti sosial pada taraf melanggar hukum negara, maka orang tua harus membawa anaknya untuk melakukan terapi gangguan kepribadian, yang disebut Terapi Perilaku Dialektikal. Sedangkan yang dapat diupayakan guru dalam menangani anak anti sosial adalah dengan menerapkan metode pembelajaran kooperatif serta memberikan perhatian Psikologi dan Perkembangan Multiple Intelegensi Anak. Selain itu masyarakat dapat memberikan kontribusi dalam penanganan anak anti sosial dengan cara menumbuhkan norma sosial yang baik serta tersedianya tayangan media massa yang memberikan tuntunan baik bagi anak.Kata Kunci : Anti – Sosial, Kooperatif, Multiple IntelegensiAnti-social behavior is a negative behavior or deviates behavior  from the norms, a good rule of family, school, community, or the law. The type of anti-social behavior in elementary school children including negativism behavior, aggression and behavioral controls. The risk factors that lead to anti-social behavior in children can be categorized as personal factors (personal risk factors, family (family risk factors, related to the school (school-related risk factors and social (social risk factors. Efforts to tackle anti-social child can do with the efforts of parents to apply parenting authoritative. If already at the level of anti-social behavior in

Prophylactic anti-D preparations display variable decreases in Fc-fucosylation of anti-D.

Science.gov (United States)

Kapur, Rick; Della Valle, Luciana; Verhagen, Onno J H M; Hipgrave Ederveen, Agnes; Ligthart, Peter; de Haas, Masja; Kumpel, Belinda; Wuhrer, Manfred; van der Schoot, C Ellen; Vidarsson, Gestur

2015-03-01

RhIG is obtained from hyperimmunized healthy anti-D donors (HIDs) boosted with D+ red blood cells (RBCs). One hypothesis for its mechanism of action is fast clearance of opsonized D+ RBCs through Fcγ receptor (FcγR)III. Levels of immunoglobulin (Ig)G Fc-fucosylation influence interactions with FcγRIII, with less Fc-fucosylation strengthening the interaction. Anti-D IgG1 Fc-glycosylation patterns in 93 plasma samples from 28 male and 28 female Dutch HIDs and RhIG were analyzed with mass spectrometry. The Fc-glycosylation profiles of HIDs were evaluated with regard to their immunization history. HID sera demonstrated clearly lowered anti-D Fc-fucosylation compared to normal IgG fucosylation (93%); this was more pronounced for female than for male HIDs (47% vs. 65%, p = 0.001). RhIG preparations from seven manufacturers varied greatly in the level of Fc-fucosylation (56%-91%). The level of fucosylation slightly increased upon repeated immunization, although it remained fairly constant over time. The RhIG from the different manufacturers all demonstrated increased Fc-galactosylation (64%-82%) compared to total IgG (38%-51%). RhIG preparations vary in Fc-fucosylation and all demonstrate increased galactosylation. Despite not knowing the exact working mechanism, immunoprophylaxis could perhaps be optimized by selection of donors whose anti-D have low amounts of Fc-fucose, to increase the clearance activity of anti-D preparations, as well as high amounts of galactosylation, for anti-inflammatory effects. Implementing a biologic assay in the standardization of RhIG preparations might be considered. © 2014 AABB.

Efeito anti-inflamatório dos macrolídeos em doenças pulmonares da infância Anti-inflammatory effects of macrolides in childhood lung diseases

Directory of Open Access Journals (Sweden)

Fernanda Luisi

2012-12-01

Full Text Available Os macrolídeos são fármacos com efeitos antimicrobianos especialmente contra patógenos intracelulares. Vários estudos têm demonstrado possíveis efeitos anti-inflamatórios dos macrolídeos. Esses medicamentos inibem a produção de algumas interleucinas e podem reduzir a inflamação neutrofílica pulmonar. Ensaios clínicos têm demonstrado efeitos benéficos dos macrolídeos em diversas doenças pulmonares crônicas. O objetivo deste estudo foi revisar os dados recentes da literatura médica sobre os efeitos anti-inflamatórios dos macrolídeos nas doenças respiratórias da infância, através da pesquisa da base de dados Medline (PubMed dos seguintes termos em inglês: "macrolide and cystic fibrosis"; "macrolide and asthma"; "macrolide and bronchiolitis obliterans"; e "macrolide and acute bronchiolitis" Foram selecionados artigos publicados em revistas científicas internacionais entre 2001 e 2012. Estudos clínicos e evidências in vitro comprovam o efeito anti-inflamatório dos macrolídeos em doenças respiratórias. Alguns ensaios clínicos demonstram benefícios na administração de macrolídeos em pacientes com fibrose cística; porém, o risco de resistência bacteriana deve ser considerado na análise desses benefícios. Tais benefícios são controversos em outras doenças respiratórias, e seu uso rotineiro não está indicado. Mais estudos clínicos controlados são necessários para avaliar a eficácia desses medicamentos como anti-inflamatórios. Dessa forma, poderemos definir melhor os benefícios dos macrolídeos no tratamento de cada uma das situações clínicas especificadas.Macrolides are drugs that have antimicrobial effects, especially against intracellular pathogens. Various studies have shown that macrolides might also have anti-inflammatory effects. Macrolides inhibit the production of interleukins and can reduce pulmonary neutrophilic inflammation. Clinical trials have demonstrated beneficial effects of

ANTI-HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN B1 (ANTI-RA33 ANTIBODIES IN RHEUMATOID ARTHRITIS AND SYSTEMIC SCLEROSIS

Directory of Open Access Journals (Sweden)

P. A. Kuznetsova

2017-01-01

Full Text Available Anti-heterogeneous nuclear ribonucleoprotein (RNP autoantibodies (AAbs are encountered in many autoimmune rheumatic diseases (ARDs. The potential diagnostic value of the RA33 AAb complex consisting of RNP A2 and alternative domains of the splicing proteins RNP B1 and RNP B2 is now of interest to rheumatologists. Subjects and methods. The authors studied the frequency of anti-RNP B1 AAbs in 300 patients with systemic ARDs, including those with rheumatoid arthritis (RA, ankylosing spondylitis (AS, systemic lupus erythematosus (SLE, systemic sclerosis (SSc, and Sjö gren's syndrome (SS and in 53 people without ARDs, who constituted a control group. Serum anti-RNP B1 AAbs were assessed by enzyme immunoassay. Results and discussion. The frequency of anti-RNP B1 AAbs in patients with ARDs was much higher than that in the control group: 170/300 (56.6% and 8/53 (13% patients, respectively. Anti-RNP B1 AAbs were detected in 78.5% (113/144 of the patients with RA; 40.3% (23/57 of those with AS, in 67.5% (27/40 of those with SSc, in 36.4% (16/44 of those with SLE, and in 13.3% (2/15 of those with SS. The diagnostic sensitivity of the marker for RA was 78.5%, its diagnostic specificity was 84.9%; the likelihood ratio of positive and negative results was 5.24 and 0.24, respectively. In the patients with RA, the level of anti-RNP B1 AAbs significantly correlated with that of C-reactive protein and erythrocyte sedimentation rate, while in those with SSc the detection of anti-RNP B1 AAbs was related to the rigidity of the vascular wall and the presence of hypertension. The frequency of anti-RNP B1 AAbs among the RA patients seronegative for rheumatoid factor and anti-cyclic citrullinated peptide antibodies was 15.4%. Conclusion. Anti-RNP B1 AAs are a useful laboratory marker (with the upper limit of the normal range being 3.3 U/ml, but are of limited value in the diagnosis of RA. Anti-RNP B1 AAbs may be regarded as an additional diagnostic marker for RA.

ESTIMACIÓN DEL EXPONENTE DE HURST Y DIMENSIÓN FRACTAL PARA EL ANÁLISIS DE SERIES DE TIEMPO DE ABSORBANCIA UV-VIS

Directory of Open Access Journals (Sweden)

Leonardo Plazas Nossa

2014-01-01

Full Text Available El objetivo de este trabajo es estimar el exponente o parámetro de Hurst y la dimensión fractal para el análisis de series de tiempo de espectrometría UV-Vis, utilizando el análisis de componentes principales PCA (Principal Component Analysis. El análisis se realiza para comprender si las series de tiempo de absorbancia UV-Vis son persistentes, anti-persistentes, determinísticas o si son ruido blanco. Se utilizaron tres diferentes series de tiempo de absorbancia UV-Vis para tres diferentes sitios de estudio: (i Planta de tratamiento de aguas residuales Salitre (PTAR en Bogotá; (ii Estación elevadora de Gibraltar en Bogotá (EEG; y (iii Planta de tratamiento de aguas residuales San Fernando (PTAR en Itagüí (sur de Medellín. Cada una de las series de tiempo tiene igual número de muestras (5705. Se redujo la dimensionalidad de los espectros de absorbancia, dada su alta correlación, con PCA y se utilizó para cada sitio de estudio la primera componente principal. Esta componente principal explicó entre el 82% al 94% de la variabilidad para los tres sitios de estudio. Se determinaron los exponentes de Hurst: (i 0.8 para PTAR Salitre; (ii 0.85 para EEG; y (iii 0.89 para PTAR San Fernando. A partir de los valores de los exponentes de Hurst se determinan las dimensiones fractales para las tres series de tiempo de absorbancia UV-Vis en los tres sitios de estudio y se obtiene en promedio una dimensión fractal de 1153. Las tres series de tiempo de absorbancia UV-Vis son persistentes y con alta auto-similitud, dado que el exponente de Hurst es mayor a 0.5.

In vitro anti-inflammatory and anti-cancer activities of Cuscuta reflexa Roxb.

Science.gov (United States)

Suresh, V; Sruthi, V; Padmaja, B; Asha, V V

2011-04-12

To determine anti-inflammatory and anti-cancer activities of Cuscuta reflexa in cell lines (in vitro). Anti-inflammatory activity of the water extract was analysed in vitro using lipopolysaccharide (LPS) induced inflammatory reactions in murine macrophage cell line RAW264.7. The expression of COX-2 and TNF-α genes involved in inflammation was analysed by SQ RT-PCR. EMSA was conducted to analyse the influence of the extract on NF-κB signalling. Anti-cancer activity was analysed on Hep3B cells by MTT assay, DAPI staining, annexin V staining and SQ-RT PCR analysis of BAX, Bcl-2, p53 and survivin. The extract down regulated LPS induced over expression of TNF-α and COX-2 in RAW264.7 cells; blocked NF-κB binding to its motifs and induced apoptosis in Hep3B cells as evidenced from MTT, DAPI staining and annexin V staining assays. The extract up regulated pro-apoptotic factors BAX and p53, and down regulated anti-apoptotic factors Bcl-2 and survivin. The study showed that Cuscuta reflexa inhibits LPS induced inflammatory responses in RAW264.7 cells through interplay of TNF-α, COX-2 and NF-κB signalling. It induced apoptosis in Hep3B cells through the up regulation of p53, BAX and down regulation of Bcl-2 and survivin. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Anti-leukemic activity and tolerability of anti-human CD47 monoclonal antibodies

Science.gov (United States)

Pietsch, E C; Dong, J; Cardoso, R; Zhang, X; Chin, D; Hawkins, R; Dinh, T; Zhou, M; Strake, B; Feng, P-H; Rocca, M; Santos, C Dos; Shan, X; Danet-Desnoyers, G; Shi, F; Kaiser, E; Millar, H J; Fenton, S; Swanson, R; Nemeth, J A; Attar, R M

2017-01-01

CD47, a broadly expressed cell surface protein, inhibits cell phagocytosis via interaction with phagocyte-expressed SIRPα. A variety of hematological malignancies demonstrate elevated CD47 expression, suggesting that CD47 may mediate immune escape. We discovered three unique CD47-SIRPα blocking anti-CD47 monoclonal antibodies (mAbs) with low nano-molar affinity to human and cynomolgus monkey CD47, and no hemagglutination and platelet aggregation activity. To characterize the anti-cancer activity elicited by blocking CD47, the mAbs were cloned into effector function silent and competent Fc backbones. Effector function competent mAbs demonstrated potent activity in vitro and in vivo, while effector function silent mAbs demonstrated minimal activity, indicating that blocking CD47 only leads to a therapeutic effect in the presence of Fc effector function. A non-human primate study revealed that the effector function competent mAb IgG1 C47B222-(CHO) decreased red blood cells (RBC), hematocrit and hemoglobin by >40% at 1 mg/kg, whereas the effector function silent mAb IgG2σ C47B222-(CHO) had minimal impact on RBC indices at 1 and 10 mg/kg. Taken together, our findings suggest that targeting CD47 is an attractive therapeutic anti-cancer approach. However, the anti-cancer activity observed with anti-CD47 mAbs is Fc effector dependent as are the side effects observed on RBC indices. PMID:28234345

Anti-foam System design description

International Nuclear Information System (INIS)

White, M.A.

1994-01-01

The Anti-foam System is a sub-system of the 242-A Evaporator facility. The Anti-foam is used within the C-A-1 Vapor-Liquid Separator, to reduce the effect of foaming and reduce fluid bumping while the vapor and liquid are separated within the C-A-1 Vapor-Liquid Separator. Excessive foaming within the vessel may possibly cause the liquid slurry mixture in the evaporator vessel to foul the de-entrainment pads and cause plant shutdown. The Anti-foam System consists of the following primary elements: the Anti-foam Tank and the Metering Pump. The upgrades to Anti-foam System include the following: installation of a new pump, instruments, and valves; and connection of the instruments, pump and agitator associated with the Anti-foam System to the Monitoring and Control System (MCS). The 242-A Evaporator is a waste treatment facility designed to reduce liquid waste volumes currently stored in the Hanford Area double shell Waste Storage Tanks. The evaporator uses evaporative concentration to achieve this volume reduction, returning the concentrated slurry to the double-shell tanks for storage and, at the same time, releasing the process effluent to a retention facilities for eventual treatment and release to the environment

Solución adyuvante CM-95 tratada magnéticamente en comparación con el adyuvante de Freund para la obtención del suero de Coombs en conejo

Directory of Open Access Journals (Sweden)

Clara Martínez

2009-12-01

Full Text Available En este trabajo se evaluó la aplicación de la solución CM-95 tratada magnéticamente como adyuvante inmunológico, en comparación con el adyuvante de Freund, para la obtención del suero de Coombs en conejos, de gran demanda en bancos de sangre y hospitales, para el diagnóstico clínico de conflictos Rh y la enfermedad hemolítica del recién nacido, entre otras. Conejos Nueva Zelanda Blancos se inocularon por la vía subcutánea con suero humano obtenido de 30 donantes O+, unido con la solución CM-95 tratada magnéticamente como adyuvante inmunológico. Se empleó un esquema de inmunización de 40 días, donde se evaluó la dinámica en el título de anticuerpos anti IgG humano por la técnica de hemoaglutinación en tubos. Al antisuero se le determinó la calidad inmunológica por el título de heteroaglutininas de los grupos sanguíneos A, B y O, de anticuerpos anticomplemento C3b, C3d y C4b y anti IgG, antes y después de su purificación. El suero de Coombs con la solución adyuvante CM-95 tratada magnéticamente mostró valores similares a los obtenidos con el adyuvante de Freund. En el mismo se cumplió con los requisitos establecidos por el Centro Estatal para el Control de la Calidad de los Medicamentos, según las normativas de la FDA para este diagnosticador. Estos resultados abren nuevas perspectivas para el uso de la solución adyuvante en la obtención del suero de Coombs.

Evaluation of monoclonal anti-A and anti-B and affinity-purified Ulex europaeus lectin I for forensic blood grouping.

Science.gov (United States)

Gaensslen, R E; Lee, H C; Carroll, J E

1984-01-01

Two different monoclonal anti-A and anti-B and several different affinity purified Ulex europaeus lectin I reagents were evaluated and compared with conventional anti-A and anti-B sera and Ulex anti-H for serologic properties, in inhibition tests with secretor salivas, and in elution tests with bloodstains. The monoclonal and purified reagents were found to be comparable to conventional ones, and accordingly suitable for forensic inhibition and elution procedures.

Busca de sensação e condutas anti-sociaise delitivas em jovens

Directory of Open Access Journals (Sweden)

Nilton Soares Formiga

Full Text Available O fenômeno das condutas juvenis tem sido alvo de interesse na ciência social e humana, principalmente aquelas que conduzem à delinqüência. Apesar de inúmeras variáveis conseguirem explicá-lo, o construto busca de sensações vem contribuindo muito para isso, pois ele enfatiza as diferenças individuais. A partir da perspectiva personalística, aponta a avaliação do grau com que os jovens pretendem viver experiências em correr riscos e sua predisposição a sensações de novidades e intensidades. 504 jovens, entre 11 e 20 anos, responderam o questionário de condutas anti-sociais e delitivas e de busca de sensação, de Arnett. Observou-se que a busca de intensidade e de novidades se relacionaram diretamente às condutas anti-sociais e delitivas. Esses resultados permitem supor que as variáveis personalísticas ainda têm bastante contribuição quando se pretende explicar a conduta juvenil delinqüente a partir da orientação de atitudes frente à excitação e ao investimento de atividades permeadoras de risco.

Anti-cyclic Citrullinated Peptide Antibody (Anti-CCP and Diagnostic Value for Rheumatoid Arthritis

Directory of Open Access Journals (Sweden)

Mehmet Agilli

2014-02-01

Full Text Available Rheumatoid arthritis (RA is an inflammatory multisystem disease of unknown etiology characterized by chronic destructive synovitis. It and #8217;s prevalence is about 1% all over the world. Serologic markers are also important beside some clinical situations upon RA diagnosis. Today, the most commonly used laboratory test is rheumatoid factor (RF in patients with suspected RA. RF is sensitive but not a specific biomarker for diagnosing RA. Early diagnosis of RA is essential to prevent of progressive joint damage. In recent years, anticyclic citrullinated peptide/protein antibody (anti-CCP attracts the attention as a remarkable biomarker for early diagnosis. Anti-CCP which is a family of anti-citrullinated protein antibodies (ACPA family, showed quite satisfactory specificity in the diagnosis of RA. Due to the prescence of ACPA was included to 2010 RA diagnostic criteria, in a manner of speaking, importance of anti-CCP was registered. [TAF Prev Med Bull 2014; 13(1.000: 83-88

Anti Ma2-associated myeloradiculopathy: expanding the phenotype of anti-Ma2 associated paraneoplastic syndromes

OpenAIRE

Murphy, Sinead M; Khan, Usman; Alifrangis, Constantine; Hazell, Steven; Hrouda, David; Blake, Julian; Ball, Joanna; Gabriel, Carolyn; Markarian, Pierre; Rees, Jeremy; Karim, Abid; Seckl, Michael J; Lunn, Michael P; Reilly, Mary M

2011-01-01

Anti-Ma2 associated paraneoplastic syndrome usually presents as limbic encephalitis in association with testicular tumours.1, 2 Only four patients have been reported with involvement outside the CNS, two of whom also had limbic or brainstem encephalitis.2, 3 We report a man with anti- Ma2 associated myeloradiculopathy and previous testicular cancer whose neurological syndrome stabilised and anti-Ma2 titres fell following orchidectomy of a microscopically normal testis.

Alternatives for recovering metals from spent catalysts for hydrotreating of heavy hydrocarbons: a case study; Alternativas para la recuperacion de metales a partir de catalizadores gastados del hidrotratamiento de hidrocarburos pesados: un caso de estudio

Energy Technology Data Exchange (ETDEWEB)

Alonso, Fernando; Ramirez, Sergio; Ancheyta, Jorge; Mavil, Martha [Instituto Mexicano del Petroleo, Mexico, D.F. (Mexico)]. E-mail: jancheyt@imp.mx

2008-05-15

The increasing production of spent hydrotreating catalysts used for processing heavy hydrocarbons and the problems related to their disposal are described in this work. These catalysts contain important amounts of heavy metals such as molybdenum (Mo), nickel (Ni), cobalt (Co) and vanadium (V), which can be recovered and hence an economical benefit may be obtained. The results of experimental tests for alkaline leaching (NaOH) to recover V and Mo, and the effect of operating conditions on metal recovery are also presented. The results show that, in general, the highest recovery of Mo is obtained at pH 8.5 and leaching time of 12 hours, while in the case of V, the highest recovery is observed at pH 9.0 and 8 hours. In both cases, the leaching solution contained 10 wt % alkaline. Based on the experimental information and data from a commercial plant, a preliminary economy study was developed, in which the expected economical benefits of metals recovery from spent catalysts used for hydrotreating heavy hydrocarbon are estimated. [Spanish] En el presente trabajo se describe la problematica de la creciente produccion de catalizadores gastados de los procesos de hidrotratamiento de hidrocarburos pesados. Estos catalizadores contienen cantidades importantes de metales pesados como molibdeno (Mo), niquel (Ni), cobalto (Co) y vanadio (V), que son susceptibles de recuperarse y obtener con ello un beneficio economico. Tambien se presentan resultados de pruebas experimentales de lixiviacion alcalina (NaOH) para la recuperacion de V y Mo, y el efecto de las variables de operacion sobre la recuperacion de metales. En general, se encontro que las mejores recuperaciones de Mo fueron a pH de 8.5 y 12 h, mientras que para el V fueron a pH de 9.0 y 8 h, ambos a una concentracion del agente lixiviante de 10% en peso. Con base en la informacion experimental obtenida y datos de una planta industrial se presenta un estudio economico preliminar, en el que se estiman los beneficios

Anti-neutrino disintegration of the deuteron

International Nuclear Information System (INIS)

Mueller, W.; Gari, M.; Max-Planck-Institut fuer Chemie

1981-01-01

The anti-neutrino disintegration of the deuteron (anti ν + D → anti ν + n + p and anti νsub(e) + D → + e + + n + n) is calculated using realistic two-body states. Meson-exchange currents are considered in the one-boson-exchange limit. The results are discussed as corrections to the cross sections obtained in effective range approximations. It is shown that the ratio of the cross sections (sigma - /sigma 0 ) for reactor antineutrinos is practically independent of the nuclear physics uncertainties. (orig.)

Role of immunoscintigraphy using Tc-99 m labelled monoclonal anti-CEA antibodies in the detection of Colorectal-rectal carcinoma

Energy Technology Data Exchange (ETDEWEB)

Ziada, G; Moustafa, H; Elhaddad, Sh; Elasser, M [Center of oncology and nuclear medicine of Kasr El-Eini Hospital, Cairo university, (Egypt)

1995-10-01

Twelve patients with colorectal carcinoma confirmed histopathologically and associated with high serum CEA level and underwent preoperative immunoscintigraphy (planar and SPECT projections) followed by operative resection with able to detect the primary tumor in colorectal region with 100% sensitivity, specificity and accuracy. Immunoscintigraphy showed sensitivity of 85%, specificity of 60% and accuracy of 66.6% in detection of para-aortic lymph nodes involved, as compared to 0%, 25% and 25% in abdominal sonography respectively. Concerning the liver involvement, there was higher sensitivity of 100% and accuracy of 91% of immunoscintigraphy in comparison to 50% and 66.6% abdominal sonography respectively. Imaging procedure at 4 and 24 hors postinjection with planar and SPECT studies were useful for proper localization of involved sites and to differentiate between malignant and benign lesions. Immunoscintigraphy is a safe procedure and the preparation of the kit of monoclonal anti-CEA anti-body is rapid with labelling efficiency more than 95% and with no adverse reaction. 3 figs.,2 tabs.

Role of immunoscintigraphy using Tc-99 m labelled monoclonal anti-CEA antibodies in the detection of Colorectal-rectal carcinoma

International Nuclear Information System (INIS)

Ziada, G.; Moustafa, H.; Elhaddad, Sh.; Elasser, M.

1995-01-01

Twelve patients with colorectal carcinoma confirmed histopathologically and associated with high serum CEA level and underwent preoperative immunoscintigraphy (planar and SPECT projections) followed by operative resection with able to detect the primary tumor in colorectal region with 100% sensitivity, specificity and accuracy. Immunoscintigraphy showed sensitivity of 85%, specificity of 60% and accuracy of 66.6% in detection of para-aortic lymph nodes involved, as compared to 0%, 25% and 25% in abdominal sonography respectively. Concerning the liver involvement, there was higher sensitivity of 100% and accuracy of 91% of immunoscintigraphy in comparison to 50% and 66.6% abdominal sonography respectively. Imaging procedure at 4 and 24 hors postinjection with planar and SPECT studies were useful for proper localization of involved sites and to differentiate between malignant and benign lesions. Immunoscintigraphy is a safe procedure and the preparation of the kit of monoclonal anti-CEA anti-body is rapid with labelling efficiency more than 95% and with no adverse reaction. 3 figs.,2 tabs

Anti-oxidant, anti-inflammatory and immunomodulating properties of an enzymatic protein hydrolysate from yellow field pea seeds.

Science.gov (United States)

Ndiaye, Fatou; Vuong, Tri; Duarte, Jairo; Aluko, Rotimi E; Matar, Chantal

2012-02-01

Enzymatic protein hydrolysates of yellow pea seed have been shown to possess high anti-oxidant and anti-bacterial activities. The aim of this work was to confirm the anti-oxidant, anti-inflammatory and immunomodulating activities of an enzymatic protein hydrolysate of yellow field pea seeds. The anti-oxidant and anti-inflammatory properties of peptides from yellow field pea proteins (Pisum sativum L.) were investigated in LPS/IFN-γ-activated RAW 264.7 NO⁻ macrophages. The immunomodulating potential of pea protein hydrolysate (PPH) was then studied in a murine model. Pea protein hydrolysate, after a 12 h pre-treatment, showed significant inhibition of NO production by activated macrophages up to 20%. Moreover, PPH significantly inhibited their secretion of pro-inflammatory cytokines, TNF-α- and IL-6, up to 35 and 80%, respectively. Oral administration of PPH in mice enhanced the phagocytic activity of their peritoneal macrophages and stimulated the gut mucosa immune response. The number of IgA+ cells was elevated in the small intestine lamina propria, accompanied by an increase in the number of IL-4+, IL-10+ and IFN-γ+ cells. This was correlated to up-regulation of IL-6 secretion by small intestine epithelial cells (IEC), probably responsible for B-cell terminal differentiation to IgA-secreting cells. Moreover, PPH might have increased IL-6 production in IECs via the stimulation of toll-like receptors (TLRs) family, especially TLR2 and TLR4 since either anti-TLR2 or anti-TLR4 was able to completely abolish PPH-induced IL-6 secretion. Enzymatic protein degradation confers anti-oxidant, anti-inflammatory and immunomodulating potentials to pea proteins, and the resulted peptides could be used as an alternative therapy for the prevention of inflammatory-related diseases.

Mimicking anti-correlations with classical interference

International Nuclear Information System (INIS)

Godoy, S; Seifert, B; Wallentowitz, S

2013-01-01

It is shown how classical laser light impinging on a beam splitter with internal reflections may mimic anti-correlations of the detected outputs, similar to those observed for anti-bunched light. The experimentally observed anti-correlation may be interpreted as a classical Hong–Ou–Mandel dip. (paper)

Anti-inflammatory profile of paricalcitol in kidney transplant recipients

Directory of Open Access Journals (Sweden)

Javier Donate-Correa

2017-11-01

Full Text Available Background and objectives: Paricalcitol, a selective vitamin D receptor activator, is used to treat secondary hyperparathyroidism in kidney transplant patients. Experimental and clinical studies in non-transplant kidney disease patients have found this molecule to have anti-inflammatory properties. In this exploratory study, we evaluated the anti-inflammatory profile of paricalcitol in kidney-transplant recipients. Methods: Thirty one kidney transplant recipients with secondary hyperparathyroidism completed 3 months of treatment with oral paricalcitol (1 μg/day. Serum concentrations and gene expression levels of inflammatory cytokines in peripheral blood mononuclear cells were analysed at the beginning and end of the study. Results: Paricalcitol significantly decreased parathyroid hormone levels with no changes in calcium and phosphorous. It also reduced serum concentrations of interleukin (IL-6 and tumour necrosis factor-alpha (TNF-α by 29% (p < 0.05 and 9.5% (p < 0.05 compared to baseline, respectively. Furthermore, gene expression levels of IL-6 and TNF-α in peripheral blood mononuclear cells decreased by 14.1% (p < 0.001 and 34.1% (p < 0.001, respectively. The ratios between pro-inflammatory cytokines (TNF-α and IL-6 and anti-inflammatory cytokines (IL-10, both regarding serum concentrations and gene expression, also experienced a significant reduction. Conclusions: Paricalcitol administration to kidney transplant recipients has been found to have beneficial effects on inflammation, which may be associated with potential clinical benefits. Resumen: Antecedentes y objetivos: El paricalcitol, un activador selectivo del receptor de la vitamina D, se utiliza en el tratamiento del hiperparatiroidismo secundario en el receptor de trasplante renal. Estudios tanto clínicos como experimentales realizados en pacientes renales no trasplantados muestran propiedades antiinflamatorias para esta molécula. En

Development of a lyophilized formulation for preparing the radiopharmaceutical 177Lu-DOTA-Anti-CD20

International Nuclear Information System (INIS)

Serrano E, L. A.

2015-01-01

The radiolabeled proteins are molecules of interest in nuclear medicine for their diagnostic and therapeutic application in cancer. Antibodies, such as chimeric monoclonal antibody Anti-CD20 rituximab, have established themselves as suitable vectors of radionuclides (e.g. 177 Lu) , introducing high affinity by the surface antigens over- expressed and widely distributed in cells involved in certain diseases. The aim of this work was to design, optimize and document the production process of radiopharmaceutical 177 Lu-DOTA-Anti-CD20 for sanitary registration request to the Comision Federal para la Proteccion contra Riesgos Sanitarios (COFEPRIS). First, a raw material analysis using the Ft-Mir technique and gamma spectrometry was performed. Then, was carried out the development of the lyophilized formulation for the preparation of 177 Lu-DOTA-Anti-CD20, in which an ANOVA was performed where the dependent variable was the radiochemical purity. The optimal pharmaceutical formulation was: 5 mg DOTA-CD20 and 80 mg Mannitol to be reconstituted with 1 m L of acetate buffer 0.25 M, ph 7, with an incubation time of 15 min at 37 degrees Celsius in a dry bath. Once completed the development of the lyophilized formulation, we proceeded to the optimization of the production process, development and validation of the analytical method. Three batches were prepared under protocols of Good Manufacturing Practice, which met pre-established specifications as sterile and endotoxin-free of bacterial formulations, with greater that 95% of radiochemical purity. Currently, is conducting the study of shelf stability. Upon completion of the stability studies, the legal record of 177 Lu-DOTA-Anti-CD20 will be integrated with documented evidence of the quality and stability of the formulation of this radiopharmaceutical. (Author)

Pharmacological Experimental Study Of The Anti-Depressant Effect ...

African Journals Online (AJOL)

Pharmacological Experimental Study Of The Anti-Depressant Effect Of Total Saikosaponins. Y Liu, C Cao, H Ding. Abstract. Background: Chai Hu has the hepato-protective, choleretic, anti-tussive, analgesic, anti-inflammatory, anti-viral, hypotensive, hypolipidemic, and anti-tumor pharmacological effects. In this study, the ...

Anti-idiotypes to anti-Lolp I (Rye) antibodies in allergic and non-allergic individuals. Influence of immunotherapy.

Science.gov (United States)

Bose, R; Marsh, D G; Delespesse, G

1986-01-01

Anti-idiotypes (aId) reacting with anti-Lol I (Lolp I; Rye I) antibodies were detected by their ability to bind to radioiodinated F(ab')2 anti-Lol I. Sera were tested after removal of anti-Lol I and anti-heavy and light chain activity by adsorption on Lol I-Sepharose 4B and normal human serum Sepharose 4B. The binding of aId to Id was inhibited by affinity purified anti-Lol I but not by certain unrelated immunoglobulins; in some sera this binding was also inhibited by Lol I. The levels of aId were measured in serial bleedings collected over a 1 year period from Lol I-sensitive patients, allergic donors not sensitive to Lol I and non-allergic persons. In Lol I-allergic patients the levels of aId were significantly influenced by seasonal exposure to pollen and by immunotherapy with extracts of grass pollen. Moreover, in 12 out of 16 cases, there was also a significant inverse relationship between changes in serum levels of aId and of IgG or IgE anti-Lol I. Most interestingly, aId were also detected in non-allergic individuals; in this case, the levels of aId were not influenced by the pollen season. The data suggest that Id-aId interactions may play a role in the regulation of anti-Lol I antibody production. PMID:3492316

Analysis of "anti-crisis strategy" definition essence and its role in enterprise anti-crisis management

Directory of Open Access Journals (Sweden)

Тетяна Олександрівна Ставерська

2016-12-01

Full Text Available The analysis of existing research of the category essence of "anti-crisis strategy" is given, the main essential features are revealed. These features are best matching its content. Existing approaches to determine the definition of "anti-crisis strategy" are systematized and summarized. Based on the allocation of essential features of anti-crisis strategy, the author’s generalized definition of this category is formulated considering the trajectory of the turbulent processes in environment

Multinationals and Anti-sweatshop Activism

OpenAIRE

Ann Harrison; Jason Scorse

2010-01-01

During the 1990s, anti-sweatshop activists campaigned to improve conditions for workers in developing countries. This paper analyzes the impact of anti-sweatshop campaigns in Indonesia on wages and employment. Identification is based on comparing the wage growth of workers in foreign-owned and exporting firms in targeted regions or sectors before and after the initiation of anti-sweatshop campaigns. We find the campaigns led to large real wage increases for targeted enterprises. There were so...

Physics of nuclear anti-shadowing

Energy Technology Data Exchange (ETDEWEB)

Schmidt, I. [Universidad Tecnica Federico Santa Maria, Dept. de Fisica, Valparaiso (Chile)

2005-07-01

Shadowing and anti-shadowing of the electromagnetic nuclear structure functions are produced by the coherence of multi-scattering quark nuclear processes. This picture leads to substantially different anti-shadowing for charged and neutral current processes, particularly in neutrino reactions, thus affecting the extraction of the weak-mixing angle sin{sup 2}({theta}{sub W}). We have found that the shadowing/anti-shadowing effect on the weak-mixing angle is approximately: {delta}sin{sup 2}({theta}{sub W}) = 0.001.

High affinity anti-TIM-3 and anti-KIR monoclonal antibodies cloned from healthy human individuals.

Directory of Open Access Journals (Sweden)

Stefan Ryser

Full Text Available We report here the cloning of native high affinity anti-TIM-3 and anti-KIR IgG monoclonal antibodies (mAbs from peripheral blood mononuclear cells (PBMC of healthy human donors. The cells that express these mAbs are rare, present at a frequency of less than one per 105 memory B-cells. Using our proprietary multiplexed screening and cloning technology CellSpot™ we assessed the presence of memory B-cells reactive to foreign and endogenous disease-associated antigens within the same individual. When comparing the frequencies of antigen-specific memory B-cells analyzed in over 20 screening campaigns, we found a strong correlation of the presence of anti-TIM-3 memory B-cells with memory B-cells expressing mAbs against three disease-associated antigens: (i bacterial DNABII proteins that are a marker for Gram negative and Gram positive bacterial infections, (ii hemagglutinin (HA of influenza virus and (iii the extracellular domain of anaplastic lymphoma kinase (ALK. One of the native anti-KIR mAbs has similar characteristics as lirilumab, an anti-KIR mAb derived from immunization of humanized transgenic mice that is in ongoing clinical trials. It is interesting to speculate that these native anti-TIM-3 and anti-KIR antibodies may function as natural regulatory antibodies, analogous to the pharmacological use in cancer treatment of engineered antibodies against the same targets. Further characterization studies are needed to define the mechanisms through which these native antibodies may function in healthy and disease conditions.

Prognostic significance of anti-p53 and anti-KRas circulating antibodies in esophageal cancer patients treated with chemoradiotherapy

International Nuclear Information System (INIS)

Blanchard, Pierre; Quero, Laurent; Pacault, Vincent; Schlageter, Marie-Helene; Baruch-Hennequin, Valerie; Hennequin, Christophe

2012-01-01

P53 mutations are an adverse prognostic factor in esophageal cancer. P53 and KRas mutations are involved in chemo-radioresistance. Circulating anti-p53 or anti-KRas antibodies are associated with gene mutations. We studied whether anti-p53 or anti-KRas auto-antibodies were prognostic factors for response to chemoradiotherapy (CRT) or survival in esophageal carcinoma. Serum p53 and KRas antibodies (abs) were measured using an ELISA method in 97 consecutive patients treated at Saint Louis University Hospital between 1999 and 2002 with CRT for esophageal carcinoma (squamous cell carcinoma (SCCE) 57 patients, adenocarcinoma (ACE) 27 patients). Patient and tumor characteristics, response to treatment and the follow-up status of 84 patients were retrospectively collected. The association between antibodies and patient characteristics was studied. Univariate and multivariate survival analyses were conducted. Twenty-four patients (28%) had anti-p53 abs. Abs were found predominantly in SCCE (p = 0.003). Anti-p53 abs were associated with a shorter overall survival in the univariate analysis (HR 1.8 [1.03-2.9], p = 0.04). In the multivariate analysis, independent prognostic factors for overall and progression-free survival were an objective response to CRT, the CRT strategy (alone or combined with surgery [preoperative]) and anti-p53 abs. None of the long-term survivors had p53 abs. KRas abs were found in 19 patients (23%, no difference according to the histological type). There was no significant association between anti-KRas abs and survival neither in the univariate nor in the multivariate analysis. Neither anti-p53 nor anti-KRas abs were associated with response to CRT. Anti-p53 abs are an independent prognostic factor for esophageal cancer patients treated with CRT. Individualized therapeutic approaches should be evaluated in this population

Prognostic significance of anti-p53 and anti-KRas circulating antibodies in esophageal cancer patients treated with chemoradiotherapy.

Science.gov (United States)

Blanchard, Pierre; Quero, Laurent; Pacault, Vincent; Schlageter, Marie-Helene; Baruch-Hennequin, Valerie; Hennequin, Christophe

2012-03-26

P53 mutations are an adverse prognostic factor in esophageal cancer. P53 and KRas mutations are involved in chemo-radioresistance. Circulating anti-p53 or anti-KRas antibodies are associated with gene mutations. We studied whether anti-p53 or anti-KRas auto-antibodies were prognostic factors for response to chemoradiotherapy (CRT) or survival in esophageal carcinoma. Serum p53 and KRas antibodies (abs) were measured using an ELISA method in 97 consecutive patients treated at Saint Louis University Hospital between 1999 and 2002 with CRT for esophageal carcinoma (squamous cell carcinoma (SCCE) 57 patients, adenocarcinoma (ACE) 27 patients). Patient and tumor characteristics, response to treatment and the follow-up status of 84 patients were retrospectively collected. The association between antibodies and patient characteristics was studied. Univariate and multivariate survival analyses were conducted. Twenty-four patients (28%) had anti-p53 abs. Abs were found predominantly in SCCE (p = 0.003). Anti-p53 abs were associated with a shorter overall survival in the univariate analysis (HR 1.8 [1.03-2.9], p = 0.04). In the multivariate analysis, independent prognostic factors for overall and progression-free survival were an objective response to CRT, the CRT strategy (alone or combined with surgery [preoperative]) and anti-p53 abs. None of the long-term survivors had p53 abs. KRas abs were found in 19 patients (23%, no difference according to the histological type). There was no significant association between anti-KRas abs and survival neither in the univariate nor in the multivariate analysis. Neither anti-p53 nor anti-KRas abs were associated with response to CRT. Anti-p53 abs are an independent prognostic factor for esophageal cancer patients treated with CRT. Individualized therapeutic approaches should be evaluated in this population.

Prognostic significance of anti-p53 and anti-KRas circulating antibodies in esophageal cancer patients treated with chemoradiotherapy

Directory of Open Access Journals (Sweden)

Blanchard Pierre

2012-03-01

Full Text Available Abstract Background P53 mutations are an adverse prognostic factor in esophageal cancer. P53 and KRas mutations are involved in chemo-radioresistance. Circulating anti-p53 or anti-KRas antibodies are associated with gene mutations. We studied whether anti-p53 or anti-KRas auto-antibodies were prognostic factors for response to chemoradiotherapy (CRT or survival in esophageal carcinoma. Methods Serum p53 and KRas antibodies (abs were measured using an ELISA method in 97 consecutive patients treated at Saint Louis University Hospital between 1999 and 2002 with CRT for esophageal carcinoma (squamous cell carcinoma (SCCE 57 patients, adenocarcinoma (ACE 27 patients. Patient and tumor characteristics, response to treatment and the follow-up status of 84 patients were retrospectively collected. The association between antibodies and patient characteristics was studied. Univariate and multivariate survival analyses were conducted. Results Twenty-four patients (28% had anti-p53 abs. Abs were found predominantly in SCCE (p = 0.003. Anti-p53 abs were associated with a shorter overall survival in the univariate analysis (HR 1.8 [1.03-2.9], p = 0.04. In the multivariate analysis, independent prognostic factors for overall and progression-free survival were an objective response to CRT, the CRT strategy (alone or combined with surgery [preoperative] and anti-p53 abs. None of the long-term survivors had p53 abs. KRas abs were found in 19 patients (23%, no difference according to the histological type. There was no significant association between anti-KRas abs and survival neither in the univariate nor in the multivariate analysis. Neither anti-p53 nor anti-KRas abs were associated with response to CRT. Conclusions Anti-p53 abs are an independent prognostic factor for esophageal cancer patients treated with CRT. Individualized therapeutic approaches should be evaluated in this population.

Anti-Bias Education: Reflections

Science.gov (United States)

Derman-Sparks, Louise

2011-01-01

It is 30 years since NAEYC published "Anti-Bias Curriculum Tools for Empowering Young Children" (Derman-Sparks & ABC Task Force, 1989). Since then, anti-bias education concepts have become part of the early childhood education (ECE) narrative in the United States and many other countries. It has brought a fresh way of thinking about…

Detection of anti-liver cytosol antibody type 1 (anti-LC1) by immunodiffusion, counterimmunoelectrophoresis and immunoblotting: comparison of different techniques.

Science.gov (United States)

Muratori, L; Cataleta, M; Muratori, P; Manotti, P; Lenzi, M; Cassani, F; Bianchi, F B

1995-12-01

Liver cytosol specific antibody type 1 (anti-LC1) was first described in a proportion of patients with liver/kidney microsomal antibody type 1 (anti-LKM1)-positive autoimmune hepatitis (AIH) and is routinely evaluated by immunodiffusion (ID). Using human liver cytosol as the source of antigen, we have used ID, counterimmunoelectrophoresis (CIE) and immunoblotting (IB), to test sera from 167 patients with documented chronic liver diseases of different etiology. 15 patients had antinuclear antibody (ANA) and/or smooth muscle antibody (SMA)-positive AIH, 13 had anti-LKM1-positive AIH, four had ANA/SMA/anti-LKM1-negative AIH, 76 had anti-LKM1-positive hepatitis C (recently renamed unclassified chronic hepatitis-UCH), 40 had chronic hepatitis C, 15 had chronic hepatitis B, and 4 had chronic hepatitis D. A precipitin line of identity with an anti-LC1 reference serum was detected both by ID and CIE in 16 patients: six with anti-LKM1-positive 'definite' AIH, four with ANA/SMA/anti-LKM1-negative 'definite' AIH, and six with anti-LKM1-positive UCH. By IB, 14 out of the 16 anti-LC1-positive sera (87.5%) reacted with a 58 kDa human liver cytosolic polypeptide, whereas three out of 16 (19%) recognised an additional 60 kDa band. Compared to ID, CIE is more economical in terms of both time and reagents and provides more clear-cut results. The 58 kDa reactivity by IB was detectable in nearly all CIE/ID anti-LC1-positive patients, was not found among CIE/ID anti-LC1-negative patients. In conclusion, CIE is the ideal screening test for the detection of anti-LC1, an autoantibody that can be regarded as an additional serological marker of AIH and is especially useful in ANA/SMA/anti-LKM1 negative cases.

Anti-Kählerian Geometry on Lie Groups

Science.gov (United States)

Fernández-Culma, Edison Alberto; Godoy, Yamile

2018-03-01

Let G be a Lie group of even dimension and let ( g, J) be a left invariant anti-Kähler structure on G. In this article we study anti-Kähler structures considering the distinguished cases where the complex structure J is abelian or bi-invariant. We find that if G admits a left invariant anti-Kähler structure ( g, J) where J is abelian then the Lie algebra of G is unimodular and ( G, g) is a flat pseudo-Riemannian manifold. For the second case, we see that for any left invariant metric g for which J is an anti-isometry we obtain that the triple ( G, g, J) is an anti-Kähler manifold. Besides, given a left invariant anti-Hermitian structure on G we associate a covariant 3-tensor 𝜃 on its Lie algebra and prove that such structure is anti-Kähler if and only if 𝜃 is a skew-symmetric and pure tensor. From this tensor we classify the real 4-dimensional Lie algebras for which the corresponding Lie group has a left invariant anti-Kähler structure and study the moduli spaces of such structures (up to group isomorphisms that preserve the anti-Kähler structures).

On Mathematical Anti-Evolutionism

Science.gov (United States)

Rosenhouse, Jason

2016-03-01

The teaching of evolution in American high schools has long been a source of controversy. The past decade has seen an important shift in the rhetoric of anti-evolutionists, toward arguments of a strongly mathematical character. These mathematical arguments, while different in their specifics, follow the same general program and rely on the same underlying model of evolution. We shall discuss the nature and history of this program and model and describe general reasons for skepticism with regard to any anti-evolutionary arguments based upon them. We shall then survey the major arguments used by anti-evolutionists, to show how our general considerations make it possible to quickly identify their weakest points.

Comparison of q anti qg and q anti qγ events in e+e- annihilation at PEP

International Nuclear Information System (INIS)

Hofmann, W.

1986-07-01

In comparing the particle flow in the event plane of three-jet (q anti qg) events and of radiative annihilation events (q anti qγ) for similar kinematic configurations, two PEP experiments find a significant decrease in particle density in the angular region opposite to the gluon jet in q anti qg events, relative to the particle density in the region opposite to the photon in q anti qγ events. The effect is predicted both by QCD and by phenomenological string models. 5 refs., 5 figs

In vitro anti-proliferative and anti-inflammatory activity of leaf and fruit extracts from Vaccinium bracteatum Thunb.

Science.gov (United States)

Landa, Premysl; Skalova, Lenka; Bousova, Iva; Kutil, Zsofia; Langhansova, Lenka; Lou, Ji-Dong; Vanek, Tomas

2014-01-01

The aim of this study was to evaluate in vitro anti-proliferative (tested on MCF-7, MDA-MB-231, and MCF-10A cell lines) and anti-inflammatory (evaluated as inhibition of prostaglandin E2 synthesis catalyzed by cyclooxygenase-2) effect of various extracts from Vaccinium bracteatum leaves and fruits. The highest anti-proliferative effect possessed leaf dichloromethane extract with IC50 values ranging from 93 to 198 μg/mL. In the case of cyclooxygenase-2 inhibition, n-hexane, dichloromethane, and ethanol fruit extracts showed the best activity with IC50 values = 2.0, 5.4, and 12.7 μg/mL, respectively. These results indicate that V. bracteatum leaves and fruits could be useful source of anti-cancer and anti-inflammatory compounds.

Insane anti-Membranes?

International Nuclear Information System (INIS)

Giecold, Gregory; Orsi, Francesco; Puhm, Andrea

2014-01-01

The backreactions of anti-branes on a variety of supergravity backgrounds have been shown in a recent series of papers to be riled by some unexplained flux singularities. All of the situations studied so far involve backgrounds with (close to) AdS-asymptotics. It is the purpose of this work to study the backreaction of anti-M2 branes on a background exhibiting a different UV behavior: the so-called A 8 regular solution of eleven-dimensional supergravity that we consider has “Taub-NUT type' asymptotics. As it turns out, some subleading infrared singularities are inevitable; they cannot be naturally ascribed to the anti-branes backreacting on this background. Moreover, our configuration does not involve smeared branes. This lends further credence to the work of Bena et al. http://dx.doi.org/10.1007/JHEP09(2013)142 suggesting that the singularities encountered are in no way remnants of smearing that would wash away once brane polarization is taken into account

Antisense gene therapy using anti-k-ras and antitelomerase oligonucleotides in colorectal cancer Eficacia de la terapia génica antisentido utilizando oligonucleótidos anti K-ras y antitelomerasa en cáncer colorrectal

Directory of Open Access Journals (Sweden)

S. Lledó

2005-07-01

Full Text Available Aim: to test the efficacy of anti-k-ras and antitelomerase oligonucleotides for disabling colorectal cancer cell growth. Material and methods: an established human colorectal cancer cell line (SW 480, ATTC® was used. Oligodeoxiribonucleotides (ODNs have a phosphorotioate modification to ensure intracellular intake. We used an antitelomerase ODN (Telp5 and two anti-k-ras ODNs (AS-KRAS and ISIS. AS-KRAS is designed to join the k-ras oncogene's exon 1. ISIS links to the terminal transcription unit 5' of k-ras. Telp5 joins the template region of the hTR telomerase subunit. ODNs have been tested in different concentrations (1, 5, 10, 20 micromolar. Cell viability has been tested at 48 and 72 hours. Statistical analysis and graphic design were made with the statistical package "Analyzing Data with GraphPad Prism-1999", GraphPad Sofware Inc., San Diego CA©. We used the Student's t test for statistical analysis. Results: the lowest dose (1 µM was not effective. Using the highest dose (20 mM for 48 hours of combined AS-KRAS and Telp5 cell viability decreased to 99.67%. The rest of results varied depending on ODN type, dose, and exposure time. Conclusions: tested antisense ODNs stop colorectal cancer cell growth, and a combination of anti-telomerase and anti-k-ras is the most useful treatment. Efficacy is best with a higher dose and longer treatment period.Objetivo: evaluar la eficacia de oligonucleótidos anti k-ras y antitelomerasa para detener el crecimiento tumoral en el cáncer colorrectal. Material y métodos: se ha empleado una línea celular establecida de cáncer colorrectal humano (SW 480, ATTC®. Los oligodesoxirribonucleótidos (ODN utilizados en el presente trabajo presentan modificación fosforotioato con el fin de mejorar su estabilidad en presencia de fluidos biológicos. Hemos utilizado un ODN antitelomerasa (Telp5, y dos ODN anti k-ras (AS-KRAS e ISIS. AS-KRAS actúa en el exón 1 e ISIS actúa a nivel de la unidad terminal de