GEL DE ALOE-VERA COMO POTENCIAL INHIBIDOR DE LA CORROSIÓN DEL ACERO DE REFUERZO ESTRUCTURAL

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Héctor Herrera-Hernández

2015-01-01

Full Text Available En el presente trabajo se estudiaelectroquímicamente un GELextracto de las hojas de ALOE VERAcomo un posible inhibidor de la corrosión del acero de refuerzo estructuraldel concreto.Los estudiosde corrosiónse llevaron a cabo en 1M de HCl y utilizando la técnica de espectroscopia de impedancia electroquímica (EISse evaluó la resistencia a la corrosión en presencia y ausencia del GEL.Los diagramas de impedancia mostraronque la adición en diferentes proporcionesdel GELinhibe notablemente el proceso de la corrosión del acero inmerso en la solución ácida. También se determinó que las moléculas del GEL obedecena un mecanismo de fisisorciónsobre la superficie del metal de acuerdo con el modelo de isoterma de Langmuircon unG°adsde alrededor de 14.17 kJ/mol. El GEL se clasificócomo un inhibidor orgánico del tipo mixto.

Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp

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Rojas Sierra2 Johanna

2011-06-01

Full Text Available Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare,Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo delgénero Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D.rotundata en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojasde M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, elmétodo de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobreseis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 yBVC 507. Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del mediopapa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 μl de cada extracto por separado. Seutilizó un control positivo con nistatina (4 mg/ml y un testigo absoluto sin ningún tipo de tratamiento.La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probardiferencias significativas entre tratamientos, cepas (aislados y tiempo de inhibición. Los resultadosexpresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de lashojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similaral control positivo

ACCIÓN NEUROPROTECTORA Y NEURORREGENERADORA DE LAS SALES DE ÁCIDOS RESÍNICOS AISLADOS DE COLOFONIA DE CONÍFERAS DEL OCCIDENTE DE CUBA

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Yasiel Arteaga-Crespo

2012-01-01

Full Text Available Las enfermedades neuropsiquiátricas afectan a más de 10 % de la población cubana y alcanzan 25 % a nivel mundial. La mayoría de los fármacos empleados para el tratamiento de trastornos del sistema nervioso central son costosos y generan severas reacciones colaterales. Actualmente, se desarrollan esfuerzos para la aplicación de metabolitos secundarios como fuentes ecológicamente sostenibles para tratamiento de patologías del sistema nervioso -incluyendo isquemias y otras neuropatologías tipo Parkinson, Alzheimer y trastornos motores de Huntington-, que permitan una neuroprotección efectiva, así como una neurorregeneración en cortos periodos, con mínimas secuelas. El objetivo del trabajo fue evaluar la acción neurorregeneradora y neuroprotectora de la sal de sodio de ácidos resínicos aislados de colofonia de coníferas cubanas; los resultados del estudio sugieren que esta sal puede utilizarse en calidad de precursores estructurales de isoanálogos bioisostéricos con acción terapéutica específica tipo neuroprotectora y neurorregeneradora en el tratamiento de patologías isquémicas.

Detección y caracterización de aislados de "escherichia coli" de origen clínico y fecal en gallinas ponedoras

OpenAIRE

Gibert Perelló, Magdalena

2011-01-01

El objetivo de esta tesis ha sido realizar la caracterización de aislados de E. coli clínicos (responsables de cuadros de colibacilosis en gallinas ponedoras) y fecales. Tras llevar a cabo el aislamiento e identificación bioquímica de los aislados, se ha procedido a la determinación del serogrupo, patotipo (detección de factores de virulencia por PCR), sensibilidad a antimicrobianos y pulsotipo (mediante la técnica de Pulsed Field-Gel Electrophoresis, PFGE). Además, se ha realizado un estudio...

Identificación y expresión diferencial del factor de transcripción asociado a microftalmia en corazón y cardiomiocitos aislados de cobayo, posible papel en hipertrofia y en viabilidad

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Luis Alberto Gómez

2014-09-01

Full Text Available Introducción. El factor de transcripción asociado a microftalmia (MITF regula la expresión de genes específicos y no se conoce su expresión y su función a nivel cardiaco. Objetivo. Identificar la expresión de MITF en corazón y en cardiomiocitos aislados de cobayo, describir cambios morfológicos asociados con su disminución y evaluar niveles relativos de su expresión en cardiomiocitos aislados en condiciones de preacondicionamiento isquémico. Materiales y métodos. El análisis de expresión relativa de la isoforma específica cardiaca, MITF-H, se determinó por PCR en tiempo real semicuantitativa, secuenciación y western blot. La disminución del mRNA de MITF se indujo con un shRNAi específico. El tamaño, el diámetro y número de fibras musculares se evaluó por observación directa microscopía de luz. Resultados. Se amplificó un fragmento de 281 pb de cDNA, el análisis de la secuencia confirmó la identidad del exón 1 y la isoforma H de MITF. La interferencia del mRNA de MITF se asoció con un mayor  índice cardiaco (peso corazón/peso corporal 5,46x10-3 vs 4,6x10-3 y un incremento del diámetro de las fibras cardiacas (50,2±16 μm vs 38,7±14,7 μm p<0.05, n= 150. En cardiomiocitos aislados en condiciones de preacondicionamiento isquémico, se observó una expresión relativa mayor de MITF-H, que en miocitos en normoxia y expuestos a lesión por isquemia simulada (ochenta y cien veces más n=5, p< 0,05 n=3. Conclusión. Los resultados sugieren que MITF-H podría estar involucrado en hipertrofia, en la respuesta al estrés por isquemia y en la supervivencia de cardiomiocitos de cobayo.

Estabilidad a la congelación descongelación de emulsiones o/w preparadas con aislados de soja nativos y desnaturalizados con diferente solubilidad

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Gonzalo Palazolo

2011-05-01

Full Text Available En este trabajo se evaluó la estabilidad a la congelación de emulsiones modelo aceite en agua (o/w preparadas con cuatro aislados de soja: dos nativos (ASN-1 y ASN-2, de similar composición pero diferente solubilidad, >90% y ~75%, respectivamente y dos desnaturalizados (ASD-1 y ASD-2, resultantes de calentar los respectivos aislados nativos (90 ‹C, 5 minutos. Las emulsiones preparadas con dispersiones acuosas al 2% p/v de ASN o ASD y aceite de girasol refinado (ƒ³ =0,25, se congelaron a -20 ‹C por 24 horas y luego se descongelaron a 20 ‹C. La estabilidad se evaluo a partir de medidas de distribución de tamano de partícula (difraccion laser y aceite liberado (AL, metodo de dilucion del colorante. Las emulsiones preparadas con ASN-2 y ASD-2 resultaron muy inestables despues de ser congeladas y descongeladas, resultadoque se evidenció por el aumento del tamano de partícula y un AL>25%. Al usar el aislado ASN-1 de mayor solubilidad, las emulsiones fueron mas estables y se observó un marcado incremento de la estabilidad cuando sus proteínas eran desnaturalizadas (ASD-1, no observándose prácticamente coalescencia ni aceite separado. En conclusión, la solubilidad y la desnaturalización proteíca de los aislados de soja son factores decisivos en la estabilidad de emulsiones frente a la congelación-descongelación.

Fungi associated with base rot disease of aloe vera (Aloe ...

African Journals Online (AJOL)

STORAGESEVER

2008-12-17

Dec 17, 2008 ... full base rot disease after 6 days of inoculation. Key words: Fungi, base rot, Aloe vera. INTRODUCTION. Aloe barbadensis Miller, popularly called Aloe vera is a phanerogame angiosperm which belongs to the family. Liliaceae. The plant is a perennial drought resistant succulent plant (Figure 1). Aloe vera is ...

Aloe vera

Energy Technology Data Exchange (ETDEWEB)

Klein, A.D.; Penneys, N.S.

1988-04-01

We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials. 27 references.

Aloe vera

International Nuclear Information System (INIS)

Klein, A.D.; Penneys, N.S.

1988-01-01

We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials. 27 references

Benzoquinona e hidroquinona preniladas y otros constituyentes aislados de Piper Bogotense C. DC.

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Luz Angela Peña

2010-11-01

Full Text Available Dos compuestos, que innovan la química conocida del género Piper, (2'E,6'E-2- famesilhidroquinona, (2'E,6'E-2-famesil- 1,4-benzoquinona, y a-D-glucosa fueron aislados de los frutos de Piper bogotense C. DC; los tallos y las hojas presentaron, como constimyentes mayoritarios, lactama del ácido 10-amino-4-hidroxi-2,3-dimetoxifenantreno carboxílico, 1,2-metilendioxi- 6-metil-4H-dibenzo lde,g] quinolina- ,5(6H-diona (Cefaradiona A, estigmasterol y sitosterol. Adicionalmente de las hojas de esta planta se aisló SC-P-D-glucopiranosil-4'-0-metilapigenina (4'-0-metilvitexina, Cytisosido o Trematina. Sus estructuras fueron determinadas por métodos espectroscópicos y comparación con datos de la literatura.

EL ALOE VERA (ALOE BARBADENSIS MILLER) COMO COMPONENTE DE ALIMENTOS FUNCIONALES

OpenAIRE

Vega G, Antonio; Ampuero C, Nevenka; Díaz N, Luis; Lemus M, Roberto

2005-01-01

Debido a la preocupación e interés actual de la población por las condiciones de salud y calidad de vida, es que ha aumentado la demanda de productos naturales, lo cual hace que las industrias farmacéuticas y de los alimentos centren sus esfuerzos en investigaciones relacionadas con su utilización. Una de las materias primas de gran demanda, tanto en el mercado externo como en el nacional, es el Aloe vera, vegetal del cual se pueden obtener productos con fines cosmetológicos, farmacéuticos y ...

Aloe

Science.gov (United States)

... the aloe plant. It is used in many skin care products. Aloe poisoning occurs when someone swallows this substance. ... Diarrhea Loss of vision Rash Severe abdominal pain Skin irritation Throat swelling (which may also cause breathing difficulty) Vomiting

Aloe Vera

Science.gov (United States)

... Health Topics A-Z # A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Aloe Vera Share: On This Page Background How Much Do ... Supplement Subset Web ... extract of Aloe barbadensis Miller (aloe vera) in F344/N rats . Toxicological Sciences. 2013;131( ...

Antibacterial activity of aloe emodin and aloin A isolated from Aloe ...

African Journals Online (AJOL)

GRACE

2006-06-02

Jun 2, 2006 ... Aloe emodin and aloin A are known to occur in both commercial viable species of aloes (Aloe vera and A. ferox), but has not previously been isolated from A. excelsa. Although extensive research had been done with most Aloe species including chemical work. (Speranza et al., 1986; Speranza et al., 1990; ...

Inestabilidad cariológica durante la formación de células madres del polen en Aloe vera (Aloaceae)

OpenAIRE

José Imery-Buiza

2007-01-01

Con el objetivo de esclarecer la posible existencia de anomalías citogenéticas que aminoren la fertilidad del polen de Aloe vera, se analizó la etapa de proliferación celular que lleva a la formación de células madres del polen (CMPs). Se recolectaron botones florales (BF) en 25 plantas de una población ubicada a 10°34’15’’ N, 64°12’08’’ W, los cuales fueron fijados en Carnoy I por 24 h y almacenados en etanol (70 % v/v). Las observaciones se realizaron en preparaciones temporales obtenidas p...

Actividad inhibitoria de un polímero de quitosana en el crecimiento vegetativo y la reproducción asexual de un aislado de Phytophthora palmivora Butler

OpenAIRE

González-Peña Fundora, Dianevys; Gómez Izaguirre, Guadalupe; Fernández Morales, Ana; Vaillant Flores, Daymara; Falcón-Rodríguez, Alejandro B.

2016-01-01

El objetivo del trabajo fue evaluar el efecto de la quitosana sobre el crecimiento vegetativo y las fases de reproducción asexual del ciclo de vida de Phytophthora palmivora Butler. Soluciones con diferentes concentraciones de quitosana, que oscilaron desde 0,5 hasta 2,5g.l-1 se mezclaron con medio de cultivo V8 antes de verterse en placas Petri, donde luego se inoculó un disco de micelio del aislado. Se evaluaron aspectos morfoculturales y el crecimiento radial cuando las colonias del tratam...

Eficacia de la crema de aloe al 25 % en la estomatitis subprotésica grado II Efficacy of 25% aloe cream in treating second degree denture stomatitis

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Idalia Camps Mullines

2007-09-01

Full Text Available Se realizó un ensayo clínico terapéutico (fase II, aleatorio, en la consulta de prótesis dental del Hospital Militar Docente “Dr. Joaquín Castillo Duany”, con el objetivo de evaluar la eficacia de la terapia antiinflamatoria con crema de aloe al 25 % sobre la mucosa afectada por estomatitis subprótesis grado II en pacientes portadores de prótesis con bases acrílicas tanto totales como parciales. El universo estuvo constituido por 120 pacientes que acudieron a nuestro servicio en el período de septiembre del 2005 a octubre del 2006. Se establecieron 2 grupos: uno de estudio y otro de control, con 60 integrantes cada uno. Al primer grupo se le aplicó la crema de aloe al 25 % hasta su curación, al segundo grupo se le indicó tratamiento convencional. En ambos se le retiraron los aparatos protésicos y se evaluaron a partir del tercer día hasta su curación. Se comprobó que la curación de la lesión se producía aproximadamente en 7 días en el grupo estudio, no así en el grupo control, donde los pacientes requirieron de un tiempo mayor para la desaparición de estos síntomas, lo que demuestra que el tratamiento con la crema de aloe al 25 % en la estomatitis subprótesis es eficaz , por lo cual recomendamos su uso a largo plazo.A phase II, randomized therapeutical clinical assay was carried out in the denture service of “Dr Joaquín Castillo Duany” teaching military hospital to evaluate the effectiveness of anti-inflammatory 25% aloe cream therapy on the mucosa affected by second degree denture stomatitis in denture wearers having total or partial acrylic supports. The universe of study was made up of 120 patients who went to this Service from September 2005 to October 2006. Two groups were formed: a study group and a control group with 60 cases each. 25% aloe cream was administered to the first group till total remission whereas the second group received the standard treatment. Dentures were removed in both groups. The

Infectividad y efectividad de rizobios aislados de Suelos de la Costa Caribe Colombiana en Vigna unguiculata

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Jonathan Alberto Mendoza Labrador

2014-07-01

Full Text Available Título en español: Infectividad y efectividad de rizobios aislados de Suelos de la Costa Caribe Colombiana  en Vigna unguiculata Título en ingles: Infectivity and effectiveness of isolated rhizobia from colombian caribbean soils in Vigna unguiculata Título corto: Infectividad y efectividad de rizobios aislados de Suelos Resumen:  Es el primer estudio en Colombia que abarca una evaluación de rizobios nativos asociados a frijol Caupí (Vigna unguiculata L. Walp. en los departamentos del Cesar y la Guajira. En esta investigación, se demostró que la utilización de  aislamientos de rizobios nativos aislados a partir de  nódulos,  mejoraron el desarrollo del frijol Caupí  (Vigna unguiculata L. Walp., siendo estas bacterias más eficientes que los tratamientos químicos y absolutos (sin inóculo ni fertilización y que las cepas inducidas mejorando además, la fijación biológica de nitrógeno y la tasa fotosintética. Como aportes del estudio, se determinó que en condiciones de invernadero la fertilización biológica fue más eficiente que la química y que, de acuerdo a los resultados obtenidos de las diferentes variables agronómicas evaluadas, esto podría influir positivamente en los rendimientos nutricionales del cultivo, base alimentaria de los sistemas ganaderos de estas regiones del país y  fuente alimenticia  de la comunidad indígena y de bajos recursos económicos.  Palabras clave Nodulación, fijación Biológica de Nitrógeno, Fertilización, Tasa Fotosintética Abstract:  This is the first study in Colombia which covers an evaluation of native rhizobium associated to the Caupí bean   (Vigna unguiculata L. Walp. in the departaments of Cesar and Guajira. In this research it was demonstrated that the use of native rhizobium isolated from nodes, improved the development of the Caupí bean (Vigna unguiculata L. Walp., being this bacteria more efficient than the chemical and absolute treatments (without inoculum and

Parámetros cinéticos como herramienta para la caracterización de aislados de Aspergillus sección Nigri

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María Silvina Sobrero

2013-12-01

Full Text Available Las especies de Aspergillus dentro de la sección Nigri son importantes en procesos biotecnológicos, así como en el biodeterioro. Bajos condiciones de cultivo controladas, la velocidad de crecimiento es una característica de las especies de hongos y algunos autores utilizan la medida del diámetro de las colonias como herramienta de identificación. El objetivo de este estudio fue determinar si la velocidad de crecimiento obtenida por la Microbiología Predictiva puede ser utilizada como herramienta para la caracterización de aspergilos negros. Se construyeron las curvas de crecimiento de 5 aislados de aspergilos negros obtenidos de alimentos y ambiente de industria láctea. Estos aislados, también fueron estudiados por sus características morfológicas y estudios de microscopía electrónica. Se identificaron como A. awamori, A. niger (2 aislados, A. foetidus y A. carbonarius. Las velocidades máximas de crecimiento en medio Agar Extracto de Malta a 25 ºC fueron: 3,51; 4,85; 4,52; 12,73; y 5,84 mm/día, respectivamente. A foetidus fue el único que presentó fase de latencia y alcanzó el mayor radio. Con la ayuda de la Microbiología Predictiva se pudieron separar A. awamori de A. foetidus, perolas diferencias no fueron significativas (p > 0,05 para los otros aislados. Los caracteres morfológicos permitieron diferenciar a A. carbonarius. Los datos cinéticos por sí solos no fueron suficientes para diferenciar especies cercanas.

Aloe vera on Lanzarote

OpenAIRE

Walker, Colin

2016-01-01

Aloe vera is the basis of a multibillion dollar cosmetics and toiletries industry worldwide. It is grown commercially in many parts of the world and here its cultivation is described on an aloe farm in the north of Lanzarote, the Canary Islands. The products from such monoculture are sold throughout the island in Aloe vera shops run principally by the company aloe Lanzarote.

CARACTERIZACIÓN MOLECULAR MEDIANTE rep-PCR DE AISLADOS NATIVOS DE Bacillus thuringiensis, OBTENIDOS DE MUESTRAS DE SUELO

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Fabián Galvis

2014-01-01

Full Text Available Bacillus thuringiensis es una bacteria Gram-positiva formadora de esporas, que produ - ce cristales parasporales de naturaleza proteica, tóxicos contra diferentes órdenes de insectos y biodegradables e inocuos para otras especies. Esta investigación empleó el modelo experimen - tal, que mediante técnicas de observación permi - tió, la identificación microbiológica y bioquímica de B. thuringiensis a partir de muestras de suelo de los municipios de Cúcuta, El Zulia, Los Patios, San Cayetano y Villa del Rosario, Norte de Santander, Colombia, y su posterior caracteri - zación con los marcadores moleculares Bc-Rep y MB1. Se identificaron microbiológica y bioquí - micamente 10 aislados como B. thuringiensis ; los resultados del análisis filogenético mostraron diferencias significativas en los agrupamientos obtenidos con los marcadores Bc-Rep y MB1. Con Bc-Rep se registró un índice de similaridad bajo (18%, mientras que con el marcador MB1 se obtuvo un índice mayor de similitud, 58%. En este trabajo se evidenció una gran variabilidad genética entre los aislados, que mostraron a los marcadores Bc-Rep y MB1 como altamente efectivos para diferenciar cepas estrechamente relacionadas, convirtiéndose en una herramienta genética de gran valor para estudios de identifi-cación y diversidad en B. thuringiensis.

Parámetros cinéticos como herramienta para la caracterización de aislados de Aspergillus sección Nigri

Directory of Open Access Journals (Sweden)

María Silvina Sobrero

2013-12-01

Full Text Available Las especies de Aspergillus dentro de la sección Nigri son importantes en procesos biotecnológicos, así como en el biodeterioro. Bajos condiciones de cultivo controladas, la velocidad de crecimiento es una característica de las especies de hongos y algunos autores utilizan la medida del diámetro de las colonias como herramienta de identificación. El objetivo de este estudio fue determinar si la velocidad de crecimiento obtenida por la Microbiología Predictiva puede ser utilizada como herramienta para la caracterización de aspergilos negros. Se construyeron las curvas de crecimiento de 5 aislados de aspergilos negros obtenidos de alimentos y ambiente de industria láctea. Estos aislados, también fueron estudiados por sus características morfológicas y estudios de microscopía electrónica. Se identificaron como A. awamori, A. niger (2 aislados, A. foetidus y A. carbonarius. Las velocidades máximas de crecimiento en medio Agar Extracto de Malta a 25 ºC fueron: 3,51; 4,85; 4,52; 12,73; y 5,84 mm/día, respectivamente. A foetidus fue el único que presentó fase de latencia y alcanzó el mayor radio. Con la ayuda de la Microbiología Predictiva se pudieron separar A. awamori de A. foetidus, pero las diferencias no fueron significativas (p > 0,05 para los otros aislados. Los caracteres morfológicos permitieron diferenciar a A. carbonarius. Los datos cinéticos por sí solos no fueron suficientes para diferenciar especies cercanas.

Aloe Vera for Tissue Engineering Applications

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Shekh Rahman

2017-02-01

Full Text Available Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

Aloe Vera for Tissue Engineering Applications.

Science.gov (United States)

Rahman, Shekh; Carter, Princeton; Bhattarai, Narayan

2017-02-14

Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications.

Science.gov (United States)

Fox, Lizelle T; du Plessis, Jeanetta; Gerber, Minja; van Zyl, Sterna; Boneschans, Banie; Hamman, Josias H

2014-04-01

To investigate the skin hydrating and anti-erythema activity of gel materials from Aloe marlothii A. Berger and A. ferox Mill. in comparison to that of Aloe barbadensis Miller (Aloe vera) in healthy human volunteers. Aqueous solutions of the polisaccharidic fractions of the selected aloe leaf gel materials were applied to the volar forearm skin of female subjects. The hydration effect of the aloe gel materials were measured with a Corneometer(®) CM 825, Visioscan(®) VC 98 and Cutometer(®) dual MPA 580 after single and multiple applications. The Mexameter(®) MX 18 was used to determine the anti-erythema effects of the aloe material solutions on irritated skin areas. The A. vera and A. marlothii gel materials hydrated the skin after a single application, whereas the A. ferox gel material showed dehydration effects compared to the placebo. After multiple applications all the aloe materials exhibited dehydration effects on the skin. Mexameter(®) readings showed that A. vera and A. ferox have anti-erythema activity similar to that of the positive control group (i.e. hydrocortisone gel) after 6 days of treatment. The polysaccharide component of the gel materials from selected aloe species has a dehydrating effect on the skin after multiple applications. Both A. vera and A. ferox gel materials showed potential to reduce erythema on the skin similar to that of hydrocortisone gel.

Diseño de un sistema semiautomático para la extracción de gel de aloe vera; análisis integral del costo del ciclo de vida del equipo.

Directory of Open Access Journals (Sweden)

Wilmer Velilla Díaz

2013-01-01

Full Text Available En el presente artículo se aborda el proceso de selección de un diseño para la extracción de gel de aloe vera. Se propuso una metodología de diseño basada en pesos ponderados, en la que se implementó un diseño conceptual, donde se identificaron las especificaciones, requerimientos y métricas, acordes a las necesidades del sector. Se definieron los elementos funcionales y físicos del sistema. A través del software CAD SolidWorks se modelaron las alternativas. Se utilizaron catálogos para la selección de los accesorios y motores y con los parámetros de operación de estos se determinaron las capacidades de producción de cada máquina. Como resultado de la metodología se seleccionaron los dos diseños que cumplen en mayor grado con las funciones y requerimientos de productividad. A las alternativas seleccionadas se les realizó el análisis del costo de ciclo de vida para escoger la alternativa definitiva, a la cual se le calculó el retorno de la inversión, con el fin de validar su viabilidad económica.

Fungi associated with base rot disease of aloe vera ( Aloe ...

African Journals Online (AJOL)

Fungi associated with base rot disease of Aloe vera (syn. Aloe barbadensis) were investigated in Niger Delta Area of Nigeria. Fungi and their percentage frequency were Aspergillus verocosa 28.03%, Fusarium oxysporium 24.24%, Plectosphaerella cucumerina 16.67%, Mammeria ehinobotryoides 15.91% and Torula ...

Primer registro de Allomyces neomoniliformis (Chytridiomycota y Dictyuchus missouriensis (Oomycota aislados de un suelo agrícola (Buenos Aires, Argentina

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Mónica M. Steciow

2001-01-01

Full Text Available Allomyces neomoniliformis y Dictyuchus missouriensis fueron aislados de un suelo agrícola conteniendo materia orgánica (hojas, raíces y tallos en Argentina. Ambas especies son citadas por primera vez para la Argentina y por segunda vez para Sudamérica, siendo el hallazgo más austral para el hemisferio occidental. Constituye el segundo aislamiento en Argentina de especies del género Allomyces y Dictyuchus. Se realizan consideraciones acerca de la distribucion de ambas especies

Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

International Nuclear Information System (INIS)

Lee, Eun Mi; Bai, Hyoung-Woo; Lee, Seung Sik; Hong, Sung Hyun; Cho, Jae-Young; Byung, Yeoup Chung

2012-01-01

Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

Science.gov (United States)

Mi Lee, Eun; Bai, Hyoung-Woo; Sik Lee, Seung; Hyun Hong, Sung; Cho, Jae-Young; Yeoup Chung, Byung

2012-08-01

Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications

Science.gov (United States)

Fox, Lizelle T.; du Plessis, Jeanetta; Gerber, Minja; van Zyl, Sterna; Boneschans, Banie; Hamman, Josias H.

2014-01-01

Objective: To investigate the skin hydrating and anti-erythema activity of gel materials from Aloe marlothii A. Berger and A. ferox Mill. in comparison to that of Aloe barbadensis Miller (Aloe vera) in healthy human volunteers. Materials and Methods: Aqueous solutions of the polisaccharidic fractions of the selected aloe leaf gel materials were applied to the volar forearm skin of female subjects. The hydration effect of the aloe gel materials were measured with a Corneometer® CM 825, Visioscan® VC 98 and Cutometer® dual MPA 580 after single and multiple applications. The Mexameter® MX 18 was used to determine the anti-erythema effects of the aloe material solutions on irritated skin areas. Results: The A. vera and A. marlothii gel materials hydrated the skin after a single application, whereas the A. ferox gel material showed dehydration effects compared to the placebo. After multiple applications all the aloe materials exhibited dehydration effects on the skin. Mexameter® readings showed that A. vera and A. ferox have anti-erythema activity similar to that of the positive control group (i.e. hydrocortisone gel) after 6 days of treatment. Conclusion: The polysaccharide component of the gel materials from selected aloe species has a dehydrating effect on the skin after multiple applications. Both A. vera and A. ferox gel materials showed potential to reduce erythema on the skin similar to that of hydrocortisone gel. PMID:24991119

Dinámica poblacional del rotífero Brachionus ibericus aislado de estanques para camarón, alimentado con diferentes dietas

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José Cristóbal Román-Reyes

2014-11-01

Full Text Available La producción de microalgas vivas para cultivar rotíferos constituye uno de los mayores costos de operación en la larvicultura de peces, por lo que se están desarrollando sustitutos comerciales de microalgas para la alimentación y producción de rotíferos. Se desconoce el efecto que tienen las formulaciones comerciales sobre la dinámica poblacional de rotíferos nativos del noroeste de México y en este estudio se evaluó el efecto de cuatro dietas comerciales (Espirulina, RotiMac®, Nanno 3600®, RotiGrow-Plus® y la microalga viva Nannochloropsis sp., sobre el crecimiento poblacional y fecundidad del rotífero Brachionus ibericus (GenBank KJ949043, aislado de una granja de camarón blanco (Litopenaeus vannamei. Se realizaron cinco réplicas por tratamiento alimenticio utilizando recipientes con volumen de 12 L de agua, con temperatura de 29 ± 1°C y salinidad de 35 ± 1, respectivamente. Los resultados mostraron que durante los periodos experimentales, las dietas probadas resultaron adecuadas para alimentar a B. ibericus, con tasas de crecimiento, tiempos de duplicación, densidades máximas y fecundidades que fluctuaron entre 0.20 a 0.27 rotíferos día-1, 2.60 a 3.42 días, 215 a 344 rotíferos mL-1 y de 0.16 a 0.39 huevos hembra-1, respectivamente. Las diferencias entre tratamientos fueron significativas (P 0.05 a las obtenidas con Nanno 3600®.

Determinación de aislados nativos de pseudomonas desulfurizadoras mediante el estudio del perfil de ácidos grasos

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Edilberto Silva Gómez

2010-09-01

Full Text Available Utilizando CGAR se determinó el contenido de ácidos grasos celulares de doce aislados colombianos, Pseudomonas aeruginosa 17, 18, 19, 20, 21, 22 y 103, Pseudomonas sp 23, 24, 25, 26 y 27 con capacidad desulfurizadora, Pseudomonas aeruginosa ATCC 9027 y 10145, Pseudomonas sp ATCC 39327 y Pseudomonas flúores cens. Se encontraron 53 ácidos grasos diferentes, entre saturados e insaturados de cadena lineal, y principalmente hidroxiácidos y ramificados.

FLAVONOIDES AISLADOS DE LAS INFLORESCENCIAS DE Piper hispidum Kunth (PIPERACEAE Y DERIVADOS ACETILADOS

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Luis Enrique Cuca

2009-04-01

Full Text Available A partir del extracto etanólico de las inflorescencias de la especie Piper hispidum Kunth (Piperaceae fueron aislados tres flavonoides: 5-hidroxi-7-metoxiflavanona, 5-hidroxi-4’,7-dimetoxiflavanona y 2’,4’,6’-trimetoxidihidrochalcona. De las flavanonas aisladas fueron obtenidos los  derivados acetilados: 5-acetoxi-7-metoxiflavanona y  5-acetoxi-4’,7-dimetoxiflavanona. Las estructuras fueron elucidadas empleando técnicas espectroscópicas y por comparación con datos de literatura. Los compuestos naturales y sus derivados fueron sometidos al bioensayo de letalidad frente a Artemia salina. El flavonoide 5-hidroxi-7-metoxiflavanona presenta la mayor actividad tóxica frente a los microcrustaceos CL50 1.8 µg/ml.

Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera

DEFF Research Database (Denmark)

Grace, Olwen Megan; Buerki, Sven; Symonds, Matthew RE

2015-01-01

Background: Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world’s largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between...... the rich traditions of medicinal use and leaf succulence in aloes. Results: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major...... succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue...

Estudio de la concentración microbiana del aire en depósitos del Archivo Nacional de Cuba

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Sofía Flavia Borrego Alonso

2010-09-01

Full Text Available Actualmente el estudio de la microbiota en ambientes interiores es de interés para los especialistas, pues los microorganismos pueden provocar afectaciones a la salud de las personas y causar el biodeterioro de colecciones valiosas. Los objetivos del trabajo fueron: determinar la concentración microbiana del aire en depósitos del Archivo Nacional de Cuba, realizar la identificación taxonómica de los hongos aislados y definir el riesgo potencial que ellos representan para el biodeterioro del patrimonio documental y la salud del personal. El muestreo microbiológico se realizó en verano e invierno y en 6 depósitos, empleando un método de sedimentación. Para aislar hongos y bacterias se emplearon placas de Petri con medios de cultivo adecuados que se colocaron por triplicado a 3m de altura. Se determinó cualitativamente la actividad celulolítica, la producción de pigmentos y de ácidos a los hongos aislados. La microbiota viable total fue mayor en invierno que en verano, siendo la concentración bacteriana significativamente superior en esta estación (sequía en tanto que la fúngica es más elevada en verano. Los géneros fúngicos que predominaron en invierno fueron Aspergillus, Cladosporium, Penicillium, Curvularia y Alternaria, mientras que en verano prevaleció Cladosporium y se aislaron los géneros Fusarium, Mucor y Chrysonilia que llegaron a alcanzar niveles significativos en algunos depósitos. Los hongos aislados degradaron celulosa, produjeron pigmentos y ácidos. Asimismo, se evidenció que pueden provocar estados alérgicos y enfermedades respiratorias a las personas. Con relación a las bacterias del aire, predominaron las Gram positivas y se identificaron los géneros Enterobacter, Hafnia, Serratia, Streptomyces, Bacillus, Streptococcus y Staphylococcus. De igual manera, se detectó que las bacterias identificadas son capaces de producir afectaciones a la salud del personal.

Eficacia de la crema de aloe al 25 % en la estomatitis subprotésica grado II

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Idalia Camps Mullines

Full Text Available Se realizó un ensayo clínico terapéutico (fase II, aleatorio, en la consulta de prótesis dental del Hospital Militar Docente “Dr. Joaquín Castillo Duany”, con el objetivo de evaluar la eficacia de la terapia antiinflamatoria con crema de aloe al 25 % sobre la mucosa afectada por estomatitis subprótesis grado II en pacientes portadores de prótesis con bases acrílicas tanto totales como parciales. El universo estuvo constituido por 120 pacientes que acudieron a nuestro servicio en el período de septiembre del 2005 a octubre del 2006. Se establecieron 2 grupos: uno de estudio y otro de control, con 60 integrantes cada uno. Al primer grupo se le aplicó la crema de aloe al 25 % hasta su curación, al segundo grupo se le indicó tratamiento convencional. En ambos se le retiraron los aparatos protésicos y se evaluaron a partir del tercer día hasta su curación. Se comprobó que la curación de la lesión se producía aproximadamente en 7 días en el grupo estudio, no así en el grupo control, donde los pacientes requirieron de un tiempo mayor para la desaparición de estos síntomas, lo que demuestra que el tratamiento con la crema de aloe al 25 % en la estomatitis subprótesis es eficaz , por lo cual recomendamos su uso a largo plazo.

Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera.

Science.gov (United States)

Grace, Olwen M; Buerki, Sven; Symonds, Matthew R E; Forest, Félix; van Wyk, Abraham E; Smith, Gideon F; Klopper, Ronell R; Bjorå, Charlotte S; Neale, Sophie; Demissew, Sebsebe; Simmonds, Monique S J; Rønsted, Nina

2015-02-26

Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world's largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between the rich traditions of medicinal use and leaf succulence in aloes. The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major radiations driven by different speciation processes, giving rise to the extraordinary diversity known today. Large, succulent leaves typical of medicinal aloes arose during the most recent diversification ~10 million years ago and are strongly correlated to the phylogeny and to the likelihood of a species being used for medicine. A significant, albeit weak, phylogenetic signal is evident in the medicinal uses of aloes, suggesting that the properties for which they are valued do not occur randomly across the branches of the phylogenetic tree. Phylogenetic investigation of plant use and leaf succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue, an adaptive feature that likely contributed to the ecological success of the genus Aloe, is the main predictor for medicinal use among Aloe species, whereas evolutionary loss of succulence tends to be associated with losses of medicinal use. Phylogenetic analyses of plant use offer potential to understand patterns in the value of global plant diversity.

PROPAGATION TECHNIQUES AND AGRONOMIC REQUIREMENTS FOR THE CULTIVATION OF BARBADOS ALOE (ALOE VERA (L. BURM. F. - A REVIEW.

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Barbara De Lucia

2016-09-01

Full Text Available Barbados aloe (Aloe vera (L. Burm. f. has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe and America.In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

ARISTOLACTAMA Y ESTERÓLES DEL TALLO DE Piper chiadoense

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Luz Angela Peña O.

2010-09-01

Full Text Available Del extracto etanólico del tallo de Piper chiadoense Junker (Piperaceae fueron aislados por métodos cromatográficos la aristolactama identificada como lactama del ácido 10-amino-3,4-dimetoxifenantreno-l carboxílico (cepharanone B; campesterol; estigmasterol y B-sitosterol. Sus estructuras fueron determinadas por métodos espectroscópicos y por comparación con datos reportados en la literatura.

Aristolactama y esteróles del tallo de piper chiadoense

OpenAIRE

Peña O., Luz Angela; P. de Díaz, Aura M.

2010-01-01

Del extracto etanólico del tallo de Piper chiadoense Junker (Piperaceae) fueron aislados por métodos cromatográficos la aristolactama identificada como lactama del ácido 10- mino-3,4-dimetoxifenantreno-l-carboxílico (cepharanone B); campesterol; estigmasterol y B-sitosterol. Sus estructuras fueron determinadas por métodos espectroscópicos y por comparación con datos reportados en la literatura.

Acemannan and Fructans from Aloe vera (Aloe barbadensis Miller) Plants as Novel Prebiotics.

Science.gov (United States)

Quezada, Maria Paz; Salinas, Carlos; Gotteland, Martin; Cardemil, Liliana

2017-11-22

The nutraceutical properties of Aloe vera have been attributed to a glucomannan known as acemannan. Recently information has been published about the presence of fructans in Aloe vera but there are no publications about acemannan and fructans as prebiotic compounds. This study investigated in vitro the prebiotic properties of these polysaccharides. Our results demonstrated that fructans from Aloe vera induced bacterial growth better than inulin (commercial FOS). Acemannan stimulated bacterial growth less than fructans, and as much as commercial FOS. Using qPCR to study the bacterial population of human feces fermented in a bioreactor simulating colon conditions, we found that fructans induce an increase in the population of Bifidobacterium spp. Fructans produced greater amounts of short chain fatty acids (SCFA), while the branched-chain fatty acids (BCFA) did not increase with these polysaccharides. Acemannan increased significantly acetate concentrations. Therefore, both Aloe vera polysaccharides have prebiotic potentials.

Process Optimization of Pontianak Aloe vera Powder (Aloe chinensis Beakker as Raw Material for Hand body lotion

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Nana Supriyatna

2010-12-01

Full Text Available Process optimization of Pontianak aloe vera powder (Aloe chinensis Beaker  as raw material for hand body lotion have been conducted on laboratory scale with the aim of obtaining the optimum conditions of processing technologies through the addition of maltodextrin and Carboxy Methyl Cellulase. The optimum results achieved at the ratio of maltodextrin to the CMC by 90%: 10%. Characteristics of aloe vera powder produced showing yellowish white color, water content of 8:56%, 5:50 pH, total solid Aloe 25.5%, weight flour ratio and fresh aloe vera leaves 1.5: 100, aloin content of 164.40 ppm, 257.08 ppm malic acid content, 10.32 ppm vitamin E and 1.4 x 105 colonies/g total bacteria. Making hand body lotion of aloe vera powder optimization results show the characteristic white color with a fragrant aroma, acidity (pH 7:07, 10.34 ppm of malic acid, 1.1 x 104 colony/g total bacteria, and quite stable while storage.

Revisión de la aloe vera (Barbadensis Miller en la dermatología actual Revision Of Aloe Vera (Barbadensis Miller In Actual Dermatology

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GM Ferraro

2009-12-01

Full Text Available El Aloe vera es una planta de uso popular en el tratamiento de algunas enfermedades de la piel y de aplicación frecuente en la cosmetología. Forma parte de diversas cremas o geles, siendo usado como antiinflamatorio y reconstituyente del tejido epitelial. Aunque se conocen varios mecanismos de acción e indicaciones clínicas, se debe realizar una revisión de las mismas para su uso, avalados por ensayos controlados para determinar su eficacia real.The Aloe vera is a popular plant used in the treatment of certain skin diseases that has a frequent application in cosmetology. It forms a part of diverse creams or gels, being used as anti-inflammatory and restorative of epithelial tissue. Although there are several mechanisms of action and clinical indications, a review of its use should be done supported by controlled trials to determine its effectiveness.

Caracterización morfoanatómica comparativa entre Aloe vera (L.) Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae)

OpenAIRE

Carpano, Stella Maris; Castro, María Teresa; Spegazzini, Etile Dolores

2009-01-01

Aloe vera (L.) Burm. F. (= Aloe barbadensis Miller) conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies d...

Anormalidades Cromosómicas en la Microsporogénesis de Aloe Vera (L.) Burm. F. (Aloaceae)

OpenAIRE

IMERY BUIZA, José; CEQUEA RUÍZ, Hernán

2002-01-01

El objetivo del presente trabajo es verificar la existencia de anormalidades cromosómicas asociadas a la baja fertilidad del polen en Aloe vera. El estudio se realizó a partir del aplastamiento de anteras inmaduras fijadas en etanol-acético y coloreadas con orceína. La viabilidad del polen se determinó en microcultivos de agar y sacarosa. Las anormalidades más frecuentes en meiosis I fueron: quiasmas persistentes entre homólogos grandes y desplazamiento precoz de cromosomas pequeños, uno o má...

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

Science.gov (United States)

Cristiano, Giuseppe; Murillo-Amador, Bernardo; De Lucia, Barbara

2016-01-01

Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems. PMID:27721816

Aristolactama y esteróles del tallo de Piper chiadoense

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Luz Angela Peña O.

2010-07-01

Full Text Available Del extracto etanólico del tallo de Piper chiadoense Junker (Piperaceae fueron aislados por métodos cromatográficos la aristolactama identificada como lactama del ácido 10- mino-3,4-dimetoxifenantreno-l-carboxílico (cepharanone B; campesterol; estigmasterol y B-sitosterol. Sus estructuras fueron determinadas por métodos espectroscópicos y por comparación con datos reportados en la literatura.

Antiplasmodial potential and quantification of aloin and aloe-emodin in Aloe vera collected from different climatic regions of India.

Science.gov (United States)

Kumar, Sandeep; Yadav, Manila; Yadav, Amita; Rohilla, Pooja; Yadav, Jaya Parkash

2017-07-17

In this study, Aloe vera samples were collected from different climatic regions of India. Quantitative HPTLC (high performance thin layer chromatography) analysis of important anthraquinones aloin and aloe-emodin and antiplasmodial activity of crude aqueous extracts was done to estimate the effects of these constituents on antiplasmodial potential of the plant. HPTLC system equipped with a sample applicator Linomat V with CAMAG sample syringe, twin rough plate development chamber (20 x 10 cm), TLC Scanner 3 and integration software WINCATS 1.4.8 was used for analysis of aloin and aloe-emodin amount. The antiplasmodial activity of plant extracts was assessed against a chloroquine (CQ) sensitive strain of P. falciparum (MRC-2). Minimum Inhibitory Concentration (MIC) of aqueous extracts of selected samples was determined according to the World Health Organization (WHO) recommended method that was based on assessing the inhibition of schizont maturation in a 96-well microtitre plate. EC (effective concentration) values of different samples were observed to predict antiplasmodial potential of the plant in terms of their climatic zones. A maximum quantity of aloin and aloe-emodin i.e. 0.45 and 0.27 mg/g respectively was observed from the 12 samples of Aloe vera. The inhibited parasite growth with EC 50 values ranging from 0.289 to 1056 μg/ml. The antiplasmodial EC 50 value of positive control Chloroquine was observed 0.034 μg/ml and EC 50 values showed by aloin and aloe-emodin was 67 μg/ml and 22 μg/ml respectively. A positive correlation was reported between aloin and aloe-emodin. Antiplasmodial activity was increased with increase in the concentration of aloin and aloe-emodin. The quantity of aloin and aloe-emodin was decreased with rise in temperature hence it was negatively correlated with temperature. The extracts of Aloe vera collected from colder climatic regions showed good antiplasmodial activity and also showed the presence of higher amount of aloin and

Skin permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox.

Science.gov (United States)

Fox, Lizelle T; Gerber, Minja; du Preez, Jan L; du Plessis, Jeanetta; Hamman, Josias H

2015-01-01

The aim of this study was to investigate the in-vitro permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox using ketoprofen as a marker compound. The permeation studies were conducted across excised female abdominal skin in Franz diffusion cells, and the delivery of ketoprofen into the stratum corneum-epidermis and epidermis-dermis layers of the skin was investigated using a tape-stripping technique. A. vera gel showed the highest permeation-enhancing effect on ketoprofen (enhancement ratio or ER = 2.551) when compared with the control group, followed by A. marlothii gel (ER = 1.590) and A. ferox whole-leaf material (ER = 1.520). Non-linear curve fitting calculations indicated that the drug permeation-enhancing effect of A. vera gel can be attributed to an increased partitioning of the drug into the skin, while A. ferox whole leaf modified the diffusion characteristics of the skin for ketoprofen. The tape stripping results indicated that A. marlothii whole leaf delivered the highest concentration of the ketoprofen into the different skin layers. Of the selected aloe species investigated, A. vera gel material showed the highest potential as transdermal drug penetration enhancer across human skin. © 2014 Royal Pharmaceutical Society.

Control biológico del hongo Sclerotium cepivorum utilizando Trichoderma asperellum en el cultivo del ajo en Costa Rica.

OpenAIRE

Rivera-Méndez, William; Zúñiga-Vega, Claudia; Brenes-Madriz, Jaime

2016-01-01

En este trabajo se evaluó la efectividad del hongo antagonista Trichoderma asperellum, previamente aislado e identificado, para el control del agente causante de la pudrición blanca del ajo y la cebolla, Sclerotium cepivorum. Para ello se realizaron ensayos de campo en seis parcelas experimentales cultivadas con ajo, donde se determinó el inóculo inicial de cada parcela. Posteriormente se aplicaron dos tratamientos distintos, uno basado en una suspensión de esporas de T. asperellum a una conc...

Caracterización del gen mecA de Staphylococcus aureus resistentes a meticilina aislados de tres grupos poblacionales de la ciudad de Medellín

Directory of Open Access Journals (Sweden)

Marcela Sanchez

Full Text Available Introducción: Staphylococcus aureus resistente a la meticilina (SARM es responsable de infecciones intrahospitalarias, las que constituyen una importante causa de morbilidad y mortalidad en nuestro medio, por lo cual la rápida identificación y tipificación molecular de la resistencia como el complejo SSCmec es esencial para entender la epidemiología de la infección. Objetivo: Caracterizar fenotípicamente la resistencia a meticilina y genotípicamente el casete cromosomal SSCmec en cepas de S. aureus aislados de individuos de la ciudad de Medellín mediante PCR múltiple. Materiales y métodos: A 41 aislamientos (hospitalarios y de la comunidad de S. aureus se les estableció la resistencia a cefoxitin mediante la técnica de Kirby-Bauer y la concentración inhibitoria mínima para oxacilina. Mediante PCR convencional se les confirmó la presencia del gen mecA. Para la tipificación del complejo SSCmec se utilizó PCR múltiple para amplificar 6 loci diferentes de este gen. Resultados: A todos los aislamientos se les confirmó resistencia a meticilina y la presencia del gen mecA, de los cuales 17 fueron clasificados como SSC mec I, 1 como SSC mec II, 21 como SSC mecIV; dos aislamientos no fue posible clasificarlos. Conclusiones: Con el uso de esta técnica clasificamos el 95% de los aislamientos del estudio, encontrando una mayor prevalencia de los SSCmec I y IV. La implementación de esta técnica permite una fácil caracterización de los aislamientos SARM y un apropiado manejo de la información de los integrantes de los comités de infecciones hospitalarios, lo cual podría impactar positivamente en el tratamiento a los pacientes y el control de enfermedades infecciosas intrahospitalarias.

Medida de distensibilidad en pulmones aislados de rata: demostración del efecto de la tensión superficial

OpenAIRE

Araya, Romina Elizabeth; De Francesco, Pablo Nicolás; Smaldini, Paola Lorena; Iarossi, M. B.; Lastra, María Laura; Pérez, Pablo Fernando; Rebolledo, Alejandro; Rinaldi, Gustavo

2016-01-01

Uno de los TP de nuestra Cátedra consiste en la medición de la distensibilidad de un pulmón aislado de rata. La interfase aire-líquido en la superficie alveolar determina la existencia de tensión superficial (TS) en el líquido, la cual disminuye la distensibilidad pulmonar (DP). Si bien el surfactante pulmonar disminuye la TS, ésta continúa siendo importante. Los métodos para medir la TS de un líquido suelen ser bastante dificultosos (Ej: balanza de torsión de Du Noüy). El objetivo de este...

Aloes of the world project

DEFF Research Database (Denmark)

Klopper, Ronell R.; Smith, Gideon F.; Crouch, Neil R.

2013-01-01

Background - The Old World genus Aloe L. comprises ± 630 species to which almost 1300 names have been applied. Members of the genus are prominent components of many, mainly arid, African landscapes. Aloes can be found in Africa (the majority of species), the Arabian Peninsula, Socotra, Madagascar...

Aloe plant extracts as alternative larvicides for mosquito control ...

African Journals Online (AJOL)

The larvicidal activity of extracts from Aloe turkanensis, Aloe ngongensis and Aloe fibrosa against the common malaria vector, Anopheles gambie, was determined. Ground Aloe leaves from the three plants were sequentially extracted with hexane, ethyl acetate, chloroform, acetone and methanol. Only the ethyl acetate ...

Aloe plant extracts as alternative larvicides for mosquito control

African Journals Online (AJOL)

SERVER

2008-04-03

Apr 3, 2008 ... Key words: Aloe, anopheles gambie, larvicidal activity. INTRODUCTION. Extracts from plants in the genus Aloe (Aloeaceae) have been widely used by pharmaceutical and cosmetic industries. Aloe species have long been known as medicinal plants (Cheney, 1970) and Aloe vera species is most widely ...

Caracterización morfoanatómica comparativa entre Aloe vera (L. Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae

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Stella Maris Carpano

Full Text Available Aloe vera (L. Burm. F. (= Aloe barbadensis Miller conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies do mesmo gênero. Isto é o que ocorre com três espécies de Aloe spp., que se cultivam ou crescem acidentalmente na Argentina: A. arborescens Mill., A. saponaria Haw. e A. ciliaris Haw. (Aloaceae. Estabeleceram-se os caracteres morfoanatômicos de diagnóstico por microscopia óptica e microscopia eletrônica de varredura (apresentam-se desenhos e fotomicrografias dos mesmos. Obtiveram-se valores numéricos dos elementos histológicos de diagnóstico: magnitudes lineares e proporcionais. Realizaram-se reações histoquímicas de identificação dos princípios ativos para sua localização "in situ".

Supercritical Carbon Dioxide extraction of Aloe Emodin and Barbaloin from Aloe Vera L. leaves and their in-vitro cytotoxic activity

International Nuclear Information System (INIS)

Kabbash, A.; El-Soud, K.A.; Zalat, E.; Shoeib, N.; Yagi, A.

2008-01-01

Aloe emodin and barbaloin, isolated as the active principles of the medicinal plant Aloe vera L., were extracted by supercritical fluid extraction (SFE) and analyzed by high performance liquid chromatography (HPLC). With optimized operating conditions for SFE, aloe emodin and barbaloin were quantitatively extracted from A. Vera leaves within 20 minutes at a flow rate of 0.3 ml/min, temperature and pressure at 40C and 3200 Psi respectively with the addition of 1 ml of methanol as a modifier. Separation of aloe emodin and barbaloin, in a pure form, from the SFE extract was achieved using a semi-preparative column. The cytotoxic activity of both aloe emodin and barbaloin were evaluated using the in-vitro MTT colorimetric assay. Aloe emodin showed a cytotoxic activity on two human colon cancer cells lines (DLD-1 and HD-29) with IC 8.94 and 10.78 M respectively, while barbaloin had no effect. (author)

Obtención y caracterización de aislados proteicos de colza

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Gonçalves, N.

1997-10-01

Full Text Available A method for the obtention of protein isolates from defatted rapeseed flour has been designed. The process includes a basic extraction followed by a precipitation at the isoelectric point of the proteins. The precipitate is washed with water (pH 4.5, ethanol and acetone, obtaining a protein isolate with 86% of protein and reducing the contents in polyphenols and soluble sugars in more than 90% with respect to the defatted flour. The final product have physico-chemical characteristics that make it atractive to be used as food and for the obtention of protein hydrolizates.

Se ha diseñado un proceso de obtención de aislados proteicos a partir de harina de colza desengrasada. El método incluye la extracción básica de las proteínas solubles seguido de una precipitación acida en el punto isoeléctrico. El precipitado es lavado con agua (pH 4.5, etanol y acetona, obteniéndose un aislado proteico con un 86% de proteína y reduciéndose el contenido en polifenoles y azúcares solubles en más de un 90% respecto a la harina desengrasada. El aislado final presenta unas características físico-químicas que lo hacen atractivo para su uso en alimentación y obtención de hidrolizados proteicos.

Topical Aloe Vera (Aloe barbadensis Miller) Extract Does Not Accelerate the Oral Wound Healing in Rats.

Science.gov (United States)

Coelho, Fernanda Hack; Salvadori, Gabriela; Rados, Pantelis Varvaki; Magnusson, Alessandra; Danilevicz, Chris Krebs; Meurer, Luise; Martins, Manoela Domingues

2015-07-01

The effect of topical application of Aloe Vera (Aloe barbadensis Miller) extract was assessed on the healing of rat oral wounds in an in vivo model using 72 male Wistar rats divided into three groups (n = 24): control, placebo and Aloe Vera (0.5% extract hydroalcoholic). Traumatic ulcers were caused in the dorsum of the tongue using a 3-mm punch tool. The Aloe Vera and placebo group received two daily applications. The animals were sacrificed after 1, 5, 10 and 14 days. Clinical analysis (ulcer area and percentage of repair) and histopathological analysis (degree of re-epithelialization and inflammation) were performed. The comparison of the differences between scores based on group and experimental period, both in quantitative and semi-quantitative analyses, was performed using the Kruskal-Wallis test. The significance level was 5%. On day 1, all groups showed predominantly acute inflammatory infiltrate. On day 5, there was partial epithelialization and chronic inflammatory infiltrate. On the days 10 and 14 total repair of ulcers was observed. There was no significant difference between groups in the repair of mouth ulcers. It is concluded that treatment using Aloe Vera as an herbal formulation did not accelerate oral wound healing in rats. Copyright © 2015 John Wiley & Sons, Ltd.

Utilization of aloe vera extract as electrolyte for an accumulator

Science.gov (United States)

Azmi, F.; Sispriatna, D.; Ikhsan, K.; Masrura, M.; Azzahra, S. S.; Mahidin; Supardan, M. D.

2018-03-01

Aloe vera contains acid, which has the potential to generate electric current. The objective of this research is to study the potency of aloe vera extract as electrolyte for an accumulator. Experimental results showed that aloe vera extract has no a stable value of voltage and currency. The voltage and currency of aloe vera extract were reduced more than 50% for 60 minutes. Then, aloe vera extract was mixed with accu zuur to produce electrolyte solution. The mixture composition of aloe vera extract to accu zuur of 50:50 (v/v) generated stable voltage and currency. The experimental results showed the potential use of aloe vera extract to reduce the chemicals used in a conventional electrolyte solution.

Mecanismo de acción antiinflamatoria de 5-O-desmetilnobiletina y derivados del catalpol en hipersensibilidad retardada.

OpenAIRE

Bas Infante, Esperanza

2007-01-01

RESUMEN La presente Tesis Doctoral se ha centrado en el estudio de 5-O-desmetilnobiletina (DMN), aislado de Sideritis tragoricanum sp. mugronensis, y los iridoides derivados del catalpol denominados escrovalentinósido (SV) y escropoliósido A (SC), aislados de Scrophularia auriculata sp. valentina. La selección de estos tres principios se basó en los estudios previos realizados en los cuales se demostraron las propiedades en modelos experimentales de inflamación aguda. Como métodos exper...

Daya Hambat Ekstrak Aloe Vera terhadap pertumbuhan Staphylococcus Aureus

OpenAIRE

Rahmat, drg.Sp,Pros

2011-01-01

Dari hasil penelitian , maka dapat disimpulkan bahwa ekstrak Aloe Vera dapat menghambat pertumbuhan bakteri Stafhylococcus aureus, dan kadar hambat minimal ekstrak Aloe Vera adalah pada konsentrasi 25%. Tujuan Penelitan Ini adalah untuk mengetahui efektifitas ekstrak Aloe vera dalam menghambat pertumbuhan bakteri Stafhylococcus aureus dan daya hambat menimal, (DHM) terhadap pertumbuhan bakteri tersebut. Metode yang digunakan adalah pertumbuhan ekstrak Aloe vera, penegnceran ekstrak , pemur...

Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

African Journals Online (AJOL)

AJB SERVER

identification of Aloin A and Aloe emodium has already been achieved in A. excelsa (Coopoosamy and Magwa,. 2006) Chrysophanol is known to occur in both commercial viable species of aloes (Aloe vera and A. ferox), but has not previously been isolated from A. excelsa. MATERIALS AND METHODS. Measurements.

Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress.

Science.gov (United States)

Salinas, Carlos; Handford, Michael; Pauly, Markus; Dupree, Paul; Cardemil, Liliana

2016-01-01

Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC). There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC) in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

Aloe vera extract activity on human corneal cells.

Science.gov (United States)

Woźniak, Anna; Paduch, Roman

2012-02-01

Ocular diseases are currently an important problem in modern societies. Patients suffer from various ophthalmologic ailments namely, conjunctivitis, dry eye, dacryocystitis or degenerative diseases. Therefore, there is a need to introduce new treatment methods, including medicinal plants usage. Aloe vera [Aloe barbadensis Miller (Liliaceae)] possesses wound-healing properties and shows immunomodulatory, anti-inflammatory or antioxidant activities. NR uptake, MTT, DPPH• reduction, Griess reaction, ELISA and rhodamine-phalloidin staining were used to test toxicity, antiproliferative activity, reactive oxygen species (ROS) reduction, nitric oxide (NO) and cytokine level, and distribution of F-actin in cells, respectively. The present study analyzes the effect of Aloe vera extracts obtained with different solvents on in vitro culture of human 10.014 pRSV-T corneal cells. We found no toxicity of ethanol, ethyl acetate and heptane extracts of Aloe vera on human corneal cells. No ROS reducing activity by heptane extract and trace action by ethanol (only at high concentration 125 µg/ml) extract of Aloe vera was observed. Only ethyl acetate extract expressed distinct free radical scavenging effect. Plant extracts decreased NO production by human corneal cells as compared to untreated controls. The cytokine (IL-1β, IL-6, TNF-α and IL-10) production decreased after the addition of Aloe vera extracts to the culture media. Aloe vera contains multiple pharmacologically active substances which are capable of modulating cellular phenotypes and functions. Aloe vera ethanol and ethyl acetate extracts may be used in eye drops to treat inflammations and other ailments of external parts of the eye such as the cornea.

Aloe vera for treating acute and chronic wounds.

Science.gov (United States)

Dat, Anthony D; Poon, Flora; Pham, Kim B T; Doust, Jenny

2012-02-15

Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process. To determine the effects of Aloe vera-derived products (for example dressings and topical gels) on the healing of acute wounds (for example lacerations, surgical incisions and burns) and chronic wounds (for example infected wounds, arterial and venous ulcers). We searched the Cochrane Wounds Group Specialised Register (9 September 2011), the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2011, Issue 3), Ovid MEDLINE (2005 to August Week 5 2011), Ovid MEDLINE (In-Process & Other Non-Indexed Citations 8 September 2011), Ovid EMBASE (2007 to 2010 Week 35), Ovid AMED (1985 to September 2011) and EBSCO CINAHL (1982 to 9 September 2011). We did not apply date or language restrictions. We included all randomised controlled trials that evaluated the effectiveness of Aloe vera, aloe-derived products and a combination of Aloe vera and other dressings as a treatment for acute or chronic wounds. There was no restriction in terms of source, date of publication or language. An objective measure of wound healing (either proportion of completely healed wounds or time to complete healing) was the primary endpoint. Two review authors independently carried out trial selection, data extraction and risk of bias assessment, checked by a third review author. Seven trials were eligible for inclusion, comprising a total of 347 participants. Five trials in people with acute wounds evaluated the effects of Aloe vera on burns, haemorrhoidectomy patients and skin biopsies. Aloe vera mucilage did not increase burn healing compared with silver sulfadiazine (risk ratio (RR) 1.41, 95% confidence interval (CI) 0.70 to 2.85). A reduction in healing time with Aloe vera was noted after haemorrhoidectomy (RR 16.33 days, 95% CI 3.46 to 77.15) and there was

Determinación del potencial promotor del crecimiento vegetal de Azotobacter spp. aislados de la rizósfera de malezas en cultivos de maíz (Zea mays L.

Directory of Open Access Journals (Sweden)

Luis H. León

2015-01-01

Full Text Available Los fertilizantes químicos representan entre 20% y 30% de los costos de producción de un cultivo, utilizados correctamente incrementan la productividad y rentabilidad; sin embargo, cada año aumenta la cantidad de fertilizantes por aplicar, debido a la deficiencia de adsorción en el suelo y absorción por la planta. Siendo el maíz el tercer cultivo de importancia en Perú, con un impacto significativo en la actividad económica y social, en el 2014, solo el 40% del maíz ofertado correspondió a la producción nacional. En busca de alternativas para disminuir el uso de fertilizantes químicos se realizan investigaciones con denominadas rizobacterias promotoras del crecimiento vegetal (PGPR, por sus siglas en ingles. Se identificaron 37 malezas en cultivos de maíz procedentes de campos agrícolas de los distritos de Monsefú y Reque, Región Lambayeque, siendo dicotiledóneas predominantes con 68 % con respecto a monocotiledóneas con 32%. Las bacterias se aislaron de la rizósfera de malezas, obteniendo 305 cultivos puros de bacterias, de los cuales 133 cultivos puros (43,7% se identificaron como Azotobacter spp., investigándose su reacción bioquímica en reducción de nitratos, utilización de sacarosa, glucosa, maltosa, manitol, ramnosa, glicerol y sorbitol, identificándose A. vinelandii (58%, A. paspali (13%, A. armeniacus (8%, A. nigricans (8% y en 13 cultivos no se identificó la especie. Con los cultivos Azotobacter spp. se cuantificó hasta 36,03 ppm de nitrógeno fijado como amonio; 60,75 ppm de ácido indol acético y 6,06 ppm de fósforo solubilizado, se determinó actividad antagónica contra Fusarium verticillioides, proteolítica y quitinolítica y. El 20% de Azotobacter spp. no afectó la emergencia de maíz amarillo duro hibrido simple AGRI- 144, el 33 % la afectó positivamente y el 47% la afectó negativamente. A su vez, ningún Azotobacter spp. afectó la sobrevivencia. Demostrándose el potencial promotor del

Eficacia de la crema de aloe al 25 % en la estomatitis subprotésica grado II Efficacy of 25% aloe cream in treating second degree denture stomatitis

OpenAIRE

Idalia Camps Mullines; Marcia Hortensia Corona Carpio; Caridad Medina Magluen; Carlos Bruzón Varona; Martha Concepción Ibáñez Castillo

2007-01-01

Se realizó un ensayo clínico terapéutico (fase II, aleatorio, en la consulta de prótesis dental del Hospital Militar Docente “Dr. Joaquín Castillo Duany”, con el objetivo de evaluar la eficacia de la terapia antiinflamatoria con crema de aloe al 25 % sobre la mucosa afectada por estomatitis subprótesis grado II en pacientes portadores de prótesis con bases acrílicas tanto totales como parciales. El universo estuvo constituido por 120 pacientes que acudieron a nuestro servicio en e...

Estudio de los metabolitos secundarios exudados por las hojas de Aloe barbadensis

OpenAIRE

López González, Germán

2016-01-01

Debido a las numerosas propiedades terapéuticas Aloe Barbadensis, como cicatrizantes, hidratantes digestivas o anti-inflamatorias; se plantea el estudio, aislamiento y determinación estructural de los componentes de su exudado. Este se obtiene al hacer un corte en la hoja, y contiene principalmente antraquinonas y glicósidos de antronas, algunos de ellos con actividad anti-cancerígena reportada. Se realizará también un estudio biodirigido del mismo y se utilizarán técnicas como la resonancia ...

O uso do Aloe sp (aloe vera em feridas agudas e crônicas: revisão integrativa

Directory of Open Access Journals (Sweden)

Lucélia Terra Chini

2017-01-01

Full Text Available Objetivos: buscar evidencias disponibles en la literatura acerca del uso de Aloe sp (sábila en la cicatrización de heridas agudas y crónicas. Materiales y método: se trata de una revisión integradora realizada en las bases de datos LILACS, PubMed y Scopus, en el periodo de febrero a marzo del 2015. La búsqueda resultó en 178 publicaciones. Resultados: siete estudios constituyeron la revisión, los cuales involucraron personas con heridas de cesárea, heridas de episiotomía, quemaduras, área donadora de injerto, heridas posthemorroidectomía y heridas fisuarias anales crónicas. Se evidenció que la sábila promueve la cicatrización de heridas, además de disminuir el dolor en fisuras anales crónicas y quemaduras. Conclusiones: la sábila representa una nueva terapéutica en el tratamiento de heridas; sin embargo, las evidencias disponibles sobre su eficacia y seguridad son ineficientes para legitimar su uso en la cicatrización de heridas agudas y crónicas; por lo tanto, no se pueden generalizar.

Caracterización de Grupos de Compatibilidad Vegetativa de Fusarium mexicanum Causante de la Malformación del Mango en Jalisco, México

OpenAIRE

Rodríguez Alvarado, Gerardo; Betancourt Resendes, Isai; Rodríguez Fernández, Rodrigo; Velázquez Monreal, José Joaquín; Fernández Pavía, Sylvia Patricia; Gómez Dorantes, Nuria

2012-01-01

Aislados de Fusarium mexicanum obtenidos de árboles de mango con la enfermedad malformación en Jalisco, fueron caracterizados de acuerdo a grupos de compatibilidad vegetativa (GCVs). F. mexicanum ha sido detectado causando malformación del mango en varias regiones de México. Los aislados fueron cultivados en medio mínimo suplementado con clorato de potasio (KClO3), NO3- y L-asparagina, para generar mutantes (nit). Los mutantes son detectados como sectores de micelio resistente al clorato, inc...

Cytotoxicity study of plant Aloe vera (Linn

Directory of Open Access Journals (Sweden)

Atul N Chandu

2012-01-01

Full Text Available Background: The objective of this study has been to evaluate the in-vitro antitumor activity of Aloe vera extract of in cultured B16F10 melanoma cell line by measuring cell viability using "Trypan blue exclusion assay" method. Aim: To find out such kind of anticancer drug which is a cheap, safe, less toxic, and more potent drug compared to chemotherapy drug. Materials and Methods: In-vitro antitumor activity cell culture1, drug treatment (standard and test extract and Trypan blue exclusion assay growth and viability test 1 were used. Treatment of Aloe vera extract against B16F10 melanoma cell line, in all concentration range, showed decrease in percent cell viability, as compared to that of negative when examined by "Trypan blue exclusion assay". Results: In overall variation of test samples, Aloe vera extract showed its best activity in the concentration of 300 μg/ml, which was approximately equal to the activity of standard drug doxorubicin. Evaluation of in-vitro antitumor activity revealed that Aloe vera extract exhibits good cytotoxic activity. The best cytotoxic activity by Aloe vera was shown at 200 μg/ml concentration. Conclusion: The study of cytoprotection against normal cells by micronucleus assay has shown that the herbal extracts have less toxic effects to the normal blood lymphocytes, as compared to that of standard anticancer drug.

Aislamiento y caracterización de microorganismos con actividad lipolítica provenientes de sedimentos del Humedal El Jaboque (Bogotá, Colombia)

OpenAIRE

Lilia Carolina Mora Moreno; Hernando Valencia Zapata

2006-01-01

En el humedal el Jaboque (Engativá) Bogotá, Colombia, se aislaron microorganismos lipolíticos provenientes de
sedimentos, mediante la utilización de medios de cultivo selectivos (agar tributirina, agar aceite de girasol). Primero se evaluó la concentración del sustrato (aceite de girasol) en la cual se encontraba el mayor número de morfotipos aislados, luego se comparó la eficiencia de los dos medios de cultivo de acuerdo a la cantidad de morfotipos aislados. Se evaluaron tres conc...

Design, formulation and evaluation of Aloe vera chewing gum

Science.gov (United States)

Aslani, Abolfazl; Ghannadi, Alireza; Raddanipour, Razieh

2015-01-01

Background: Aloe vera has antioxidant, antiinflammatory, healing, antiseptic, anticancer and antidiabetic effects. The aim of the present study was to design and evaluate the formulation of Aloe vera chewing gum with an appropriate taste and quality with the indications for healing oral wounds, such as lichen planus, mouth sores caused by cancer chemotherapy and mouth abscesses as well as reducing mouth dryness caused by chemotherapy. Materials and Methods: In Aloe vera powder, the carbohydrate content was determined according to mannose and phenolic compounds in terms of gallic acid. Aloe vera powder, sugar, liquid glucose, glycerin, sweeteners and different flavors were added to the soft gum bases. In Aloe vera chewing gum formulation, 10% of dried Aloe vera extract entered the gum base. Then the chewing gum was cut into pieces of suitable sizes. Weight uniformity, content uniformity, the organoleptic properties evaluation, releasing the active ingredient in the phosphate buffer (pH, 6.8) and taste evaluation were examined by Latin square method. Results: One gram of Aloe vera powder contained 5.16 ± 0.25 mg/g of phenolic compounds and 104.63 ± 4.72 mg/g of carbohydrates. After making 16 Aloe vera chewing gum formulations, the F16 formulation was selected as the best formulation according to its physicochemical and organoleptic properties. In fact F16 formulation has suitable hardness, lack of adhesion to the tooth and appropriate size and taste; and after 30 min, it released more than 90% of its drug content. Conclusion: After assessments made, the F16 formulation with maltitol, aspartame and sugar sweeteners was selected as the best formulation. Among various flavors used, peppermint flavor which had the most acceptance between consumers was selected. PMID:26605214

In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel.

Science.gov (United States)

Kang, Min-Cheol; Kim, Seo Young; Kim, Yoon Taek; Kim, Eun-A; Lee, Seung-Hong; Ko, Seok-Chun; Wijesinghe, W A J P; Samarakoon, Kalpa W; Kim, Young-Sun; Cho, Jin Hun; Jang, Hyeang-Su; Jeon, You-Jin

2014-01-01

The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications. Copyright © 2013 Elsevier Ltd. All rights reserved.

Isolation of volatile compounds of Aloe excelsa (Berger)

African Journals Online (AJOL)

hope&shola

2010-10-25

Oct 25, 2010 ... carried out on Aloe ferox, Aloe vera as well as A. excelsa. (Speranza et al., 1986 ... perfumes, soaps, foods, chewing gums and beverages. (Venkatsen et al. ... excelsa gel is assumed to enhance the skin defense mechanisms.

Aloe vera: A review of toxicity and adverse clinical effects.

Science.gov (United States)

Guo, Xiaoqing; Mei, Nan

2016-04-02

The Aloe plant is employed as a dietary supplement in a variety of foods and as an ingredient in cosmetic products. The widespread human exposure and its potential toxic and carcinogenic activities raise safety concerns. Chemical analysis reveals that the Aloe plant contains various polysaccharides and phenolic chemicals, notably anthraquinones. Ingestion of Aloe preparations is associated with diarrhea, hypokalemia, pseudomelanosis coli, kidney failure, as well as phototoxicity and hypersensitive reactions. Recently, Aloe vera whole leaf extract showed clear evidence of carcinogenic activity in rats, and was classified by the International Agency for Research on Cancer as a possible human carcinogen (Group 2B). This review presents updated information on the toxicological effects, including the cytotoxicity, genotoxicity, carcinogenicity, and adverse clinical effects of Aloe vera whole leaf extract, gel, and latex.

Hypoglycemic effect of instant aloe vera on the diabetic rats

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Riyanto

2017-09-01

Full Text Available Instant aloe vera contains phenolic compounds which has antioxidative activity. However, this product is hygroscopic and damaged easily during storage. The critical condition of the instant occurs at the moisture content of 12.52 ± 0.24% (wb. Increasing the moisture content could accelerate oxidation of the phenolic compounds, thus decrease the antioxidative activity. Previous research showed that the antioxidative activity of instant aloe vera could lower the blood glucose. The purpose of this study was to evaluate the hypoglycemic activity of instant aloe vera during storage until the critical condition. The hypoglycemic effect was determined with the in vivo method using diabetic Wistar rats as experimental animals. The diabetic rats were fed with a standard feed combined with instant aloe vera which has been stored at various storage time i.e. 0, 2, 4, 6, 8 weeks and used normal rats fed without instant aloe vera as a control. The blood glucose was analyzed every week until 4 weeks. The research showed that the diabetic rats fed with standard feed without instant aloe vera had high blood glucose (219.40 mg/dL after 4 weeks treatment. Otherwise, the blood glucose of diabetic rats fed with instant aloe vera decreased from 214.00 mg/dL to 97.57 mg/dL after 4 weeks.

Resistencia y susceptibilidad de microorganismos aislados en pacientes atendidos en una institución hospitalaria de tercer nivel, Villavicencio-Colombia, 2012

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Oscar Alexander Gutiérrez Lesmes

2015-05-01

Full Text Available Introducción: La resistencia bacteriana es problema significativo de salud pública y está asociada al uso indiscriminado e irracional de antibióticos, lo que afecta la eficacia de los tratamientos. Objetivo: Estimar la resistencia antimicrobiana a los antibióticos resultado de las pruebas invitro de susceptibilidad en una institución hospitalaria de tercer nivel de Villavicencio, Colombia. Materiales y Métodos: Se realizó un estudio observacional, descriptivo de tipo retrospectivo, análisis univariado, calculando medias de las variables susceptibilidad, resistencia, antibiótico, microorganismos, y tipo de muestra resultados de las pruebas de susceptibilidad realizadas mediante técnica de Kirby-Bauer de 485 pruebas de susceptibilidad de la institución hospitalaria. Resultados y Discusión: Los microorganismos más frecuentemente aislados, fueron: Escherichia coli, Klebsiella pneumoniae ss. Pneumoniae, Staphylococcus aureus ss. Aureus representando el 49% de total de microorganismos, los mayores reportes de resistencia en la pruebas invitro las presentaron Serratia marcescens, Staphylococcus saprophyticus ss. Saprophytic, Enterobacter cloacae, presentan una resistencia media superior al 50%, y una susceptibilidad inferior 40%, la mayor resistencia media se presentó para los siguientes antibióticos, Cefalotina 72,2%, Cefazolina 59,9%, Nitrofurantoina 54,4%, Ceftriaxona 52,7%. Conclusiones: Antibióticos como la Cefalotina, Nitrofurantoina, Cefazolina y Ceftriaxona  pierden su utilidad terapéutica dada la elevada resistencia demostrada por los microorganismos aislados en las pruebas invitro, es necesario reforzar las medidas de uso adecuado de antibióticos para disminuir la posibilidad de adaptación y resistencia a los mismos,  el fenómeno de resistencia bacteriana ocurre fuera del ámbito hospitalario evidenciando la necesidad de iniciar también control y vigilancia en infecciones ocurridas en la comunidad.Cómo citar este

Formulation and Characterization of Aceclofenac -Aloe vera Transemulgel.

Science.gov (United States)

Raju, Y Prasanna; Haritha, K; Satyanarayana, Rao P; Vandana, K R; Bindu, D Thushara; Vinesha, V; Chowdary, V Harini

2015-01-01

The present research was aimed to formulate aceclofenac transemulgel using Aloe vera as gel base. The prepared formulations were subjected to physical characterization, in-vitro and in-vivo assessment. Aceclofenac, a hydrophobic potential non steroidal anti inflammatory drug, causes ulceration upon chronic oral administration, could be formulated into transemulgel to enhance therapeutic efficacy and to lower the unwanted side effects. The transemulgel was prepared from aqueous Aloe vera gel and aceclofenac emulsion. The prepared transemulgel was evaluated for its pH, viscosity, drug content, skin irritation, in-vitro diffusion and accelerated stability studies. The prepared aceclofenac-Aloe vera tranemulgel and commercial aceclofenac gel were subjected to pharmacodynamic studies in albino rats of Wistar strain employing carrageenan induced left hind paw edema method to assess the anti-inflammatory effect. The transemulgel showed a pH of 6.78 and viscosity of 18 cps. In-vitro diffusion data revealed better permeation characteristics. Topical application of formulation found no skin irritation. Stability study has proved the integrity of the formulation. The prepared aceclofenac Aloe vera transemulgel showed better in-vitro drug release when compared with the commercial aceclofenac gel formulation. Anti-inflammatory activity in treated rats showed the significant paw volume reduction at pAloe vera as gel base.

Aloe in Angola (Asphodelaceae: Alooideae

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R. R. Klopper

2009-08-01

Full Text Available Botanical exploration of Angola was virtually impossible during the almost three-decade-long civil war. With more areas becoming accessible, there is, however, a revived interest in the flora of this country. A total of 27 members of the genus Aloe L. have been recorded for Angola. It is not unlikely that new taxa will be discovered, and that the distribution ranges of others will be expanded now that botanical exploration in Angola has resumed. This manuscript provides a complete taxonomic treatment of the known Aloe taxa in Angola. It includes, amongst other information, identification keys, descriptions and distribution maps.

Antifungal activity of aloe vera gel against plant pathogenic fungi

International Nuclear Information System (INIS)

Sitara, U.; Hassan, N.; Naseem, J.

2011-01-01

Aloe vera gel extracted from the Aloe vera leaves was evaluated for their antifungal activity at the rate of 0.15%, 0.25% and 0.35% concentration against five plants pathogenic fungi viz., Aspergillus niger, Aspergillus flavus, Alternaria alternata, Drechslera hawaiensis and Penicillum digitatum 0.35% concentration Aloe vera gel completely inhibited the growth of Drechslera hawaiensis and Alternaria alternata. (author)

Qualitative improvement of low meat beef burger using Aloe vera.

Science.gov (United States)

Soltanizadeh, Nafiseh; Ghiasi-Esfahani, Hossein

2015-01-01

Low meat beef burgers have found their niche in the food markets in developing countries because of their lower price. However, these burgers still lack an acceptable quality. This study investigates the effects of different concentrations of Aloe vera on the quality of this food product. For this purpose, beef burgers were produced with 0%, 1%, 3%, and 5% Aloe vera and the changes in their cooking parameters, lipid oxidation, texture, and appeal to consumers over 7days of refrigerated storage were evaluated. Results indicate that Aloe vera contributed to some extent to decreased cooking loss and diameter reduction in the burgers. Increased concentrations of Aloe vera led to improvements in the water absorption and texture of the burgers as well as their lipid stability. However, a concentration level of 3% led to the most acceptability of the product to the panelists. Finally, it was found that Aloe vera acts as a hydrocolloid and improves the quality of burgers. Copyright © 2014 Elsevier Ltd. All rights reserved.

Efficacy of orally administered powdered aloe juice (Aloe ferox against ticks on cattle and ticks and fleas on dogs

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J.J. Fourie

2005-06-01

Full Text Available The efficacy of orally administered powdered aloe juice (Aloe ferox was evaluated against ticks on cattle and against ticks and fleas on dogs. Twelve calves were each infested over a 25-day period with approximately 4000 larvae of Rhipicephalus (Boophilus decoloratus and allocated to 3 groups of 4 calves each. Three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg / kg body weight and those in another 25 mg / kg body weight of powdered aloe juice incorporated in game maintenance pellets, while the animals in the 3rd group received only pellets. Detached female ticks were collected daily and counted and the weights and the fertility of groups of 50 engorged female ticks collected from the animals were ascertained. The powdered aloe juice in the game maintenance pellets had no effect on the tick burdens of the calves or on the fertility of the ticks. Six dogs, in each of 2 groups, were treated daily for 15 consecutive days, commencing on Day -5 before the 1st tick infestation, with either 0.39 g or 0.74 g of powdered aloe juice, administered orally in gelatin capsules, while a 3rd group of 6 dogs served as untreated controls. All the dogs were challenged with Haemaphysalis leachi on Days 0 and +7, and with Ctenocephalides felis on Days+1and +8, and efficacy assessments were made 1 day after flea and 2 days after tick challenge, respectively. Treatment was not effective against ticks or fleas on the dogs.

Quality change of apple slices coated with Aloe vera gel during storage.

Science.gov (United States)

Song, Hye-Yeon; Jo, Wan-Shin; Song, Nak-Bum; Min, Sea C; Song, Kyung Bin

2013-06-01

Fresh-cut apples are easily susceptible to browning and microbial spoilage. In this study, an edible coating prepared from Aloe vera gel containing antibrowning solution was applied to preserve the quality of fresh-cut apples during storage. Fresh-cut apples were treated with both an Aloe vera gel and an Aloe vera gel containing 0.5% cysteine and then stored at 4 °C for 16 d. The color, firmness, weight loss, soluble solid content, titratable acidity, microbial analysis, and sensory evaluation were analyzed during storage. Fresh-cut apples coated with the Aloe vera gel showed delayed browning and reduced weight loss and softening compared to the control. The Aloe vera gel coating was also effective in reducing the populations of the total aerobic bacteria and yeast and molds. In particular, Aloe vera gel containing 0.5% cysteine was most effective in delaying browning and the reduction of microbial populations among the treatments. These results suggest that an Aloe vera gel coating can be used for maintaining the quality of fresh-cut apples. © 2013 Institute of Food Technologists®

[GPC Fingerprint Chromatograms of Aloe vera Leaf Gel Polysaccharides].

Science.gov (United States)

Wang, Qiao-e; Xie, Dan; Qian, Jie; Dong, Yin-mao

2015-10-01

To establish the gel permeation chromatography (GPC) fingerprint chromatograms of polysaccharides in Aloe vera leaf gel from the same habitat (Beijing) and different habitats for evaluating the quality of Aloe vera leaf gel products commercially available and testing common adulterated substances. The samples were prepared by water-extraction and alcohol-precipitation method. GPC separation was performed on a Shodex SUGAR KS-805 (300 mm x 8.0 mm, 7 μm) column and a Shodex SUGAR KS-803 (300 mm x 8.0 mm, 6 μm) column at the temperature of 60 degrees C by eluting with 0.1 mol/L NaNO3 (containing 0.2 per thousand NaN) at a flow rate of 0.8 mL/min, the chromatographic effluent was detected by refractive index detector (RID) at the temperature of 50 degrees C. The common pattern of GPC fingerprint chromatograms was established and four common peaks were demarcated. The similarities of samples from the same habitat (Beijing) and different habitats were over 0.9. Taking the GPC fingerprint chromatograms for the qualified model, three commercially available aloe products were evaluated to be made of Aloe vera by the different manufacturing processes and four common adulterated substances of aloe polysaccharides were identified effectively. The method is simple and accurate with a good reproducibility, and it can be used for the identification and quality evaluation of Aloe vera leaf gel products.

Atributos biológicos y terapéuticos de la Sábila (Aloe vera | Biological and therapeutic attributes of Aloe (Aloe vera

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José Imery Buzia

2017-11-01

Full Text Available Aloe, Aloe vera (L. Burm. f. (= A. barbadensis Miller is a monocotyledon, perennial, and succulent plant of the family Asphodelaceae, recognized worldwide for its medicinal properties and ornamental value. For the beauty of their vegetative structures, drought tolerance, popular beliefs and mysticism, Aloe has an important place in the house porches, gardens, terraces, and balconies of many Latin American households. Its healing potential has been supported by multiple laboratories, natural medicine and traditional hospitals that evaluate alternatives for healing of gastrointestinal, epidermal, cardiovascular, respiratory, metabolic, cancer, among other, which are reported to improve the rational management of extracts as a specific treatment to relieve some diseases or as an adjunct to the effects of formal medicine. This work presents a compendium of botanical studies by our research laboratory on local populations of A. vera and provide an update review on its taxonomy, phylogeny, components, and therapeutic uses. Specific anatomical details and morphological traits, evaluated under ambient conditions of tropical very dry forest of northeastern Venezuela, were described and scientific arguments about its origin, evolution and nomenclature were analyzed. This is a contribution to the comprehensive knowledge of this species, whose cultivation and agro-industrial use represents a possibility to improve the quality of life of coastal communities, diversification of local economies and the export of non-traditional products.

Actividad antioxidante de compuestos aislados del residuo no-volátil del aceite esencial de naranja.

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Vargas-Arispuro, L.

1998-04-01

Full Text Available There has been a growing interest in the food industry and in preventive medicine to search for «natural antioxidants». Therefore the objetive of this study was to evaluate the antioxidant activity of the non-volatile residue of essential orange oil in the oxidation of unrefined and freshly extracted olive oil. Essential orange oil was distilled to obtain a non-volatile residue and fracctionated on a Silica Gel 60 column. The antioxidant activity was determined by measuring peroxide value obtained after heating olive oil at 70 °C for 48 h. Six compounds were isolated and purified, four of these had antioxidant activity, α-tocopherol and three methoxylated flavones were identified using spectroscopic methods. This study confirmed that non-volatile fractions of essential orange oil contain natural antioxidants diferent from α-tocopherol.

Existe un creciente interés en la industria alimentaria y en la medicina preventiva por la búsqueda de «antioxidantes naturales», por lo que nos planteamos evaluar la actividad antioxidante del residuo no volátil del aceite esencial de naranja contra la oxidación del aceite de oliva sin retinar y de reciente extracción. Mediante destilación del aceite esencial de naranja se obtuvo el residuo no-volátil, el cual se fraccionó en columna de silica gel 60. La actividad antioxidante fue medida por el valor de peróxidos producidos por el aceite de oliva al provocar la oxidación por calentamiento a 70 °C durante 48 h. Se aislaron seis compuestos mediante HPLC preparativo de los cuales cuatro tuvieron actividad antioxidante. Usando métodos espectroscópicos se identificaron α-tocoferol y tres flavonas metoxiladas. Con este estudio se confirmó que la fracción no volátil del aceite esencial de naranja contiene antioxidantes naturales diferentes al α-tocoferol.

La metamorfosis del río Turia en Valencia (1897-2016): de cauce torrencial urbano a corredor verde metropolitano

OpenAIRE

Portugués Mollá, Iván

2017-01-01

La presente tesis estudia la profunda metamorfosis del cauce y las orillas del Turia a su paso por Valencia a lo largo del siglo XX y principios del XXI. En gran medida se ha producido de forma paralela a los cambios urbanos y metropolitanos. Esta simultaneidad de interrelaciones entre río y ciudad no representa un caso aislado, sino que plasma muchas de las problemáticas que aquel binomio plantea en el ambiente mediterráneo español (escasez de recursos, prácticas abusivas, contaminación y de...

Variation in Phytochemical Composition Reveals Distinct Divergence of Aloe vera (L.) Burm.f. From Other Aloe Species: Rationale Behind Selective Preference of Aloe vera in Nutritional and Therapeutic Use

Science.gov (United States)

Dey, Priyankar; Dutta, Somit; Chowdhury, Anurag; Das, Abhaya Prasad; Chaudhuri, Tapas Kumar

2017-01-01

In the present study, we have phytochemically characterized 5 different abundant Aloe species, including Aloe vera (L.) Burm.f., using silylation followed by Gas Chromatography-Mass Spectrometry technique and compared the data using multivariate statistical analysis. The results demonstrated clear distinction of the overall phytochemical profile of A vera, highlighted by its divergent spatial arrangement in the component plot. Lowest correlation of the phytochemical profiles were found between A vera and A aristata Haw. (−0.626), whereas highest correlation resided between A aristata and A aspera Haw. (0.899). Among the individual phytochemicals, palmitic acid was identified in highest abundance cumulatively, and carboxylic acids were the most predominant phytochemical species in all the Aloe species. Compared to A vera, linear correlation analysis revealed highest and lowest correlation with A aspera (R 2 = 0.9162) and A aristata (R 2 = 0.6745), respectively. Therefore, A vera demonstrated distinct spatial allocation, reflecting its greater phytochemical variability. PMID:29228808

Desarrollo de un alimento funcional deshidratado constituido por microorganismos aislados de Kefir

OpenAIRE

Bolla, Patricia Araceli

2011-01-01

A pesar de los numerosos estudios sobre probióticos que encontramos en la actualidad, poco se conoce respecto a complejos multicepas. En este trabajo de tesis se propuso: 1- Desarrollar una mezcla microbiana constituida por microorganismos aislados de kefir. 2- Estudiar sus características tecnológicas y potencialmente probióticas. 3- Determinar la capacidad inhibitoria frente a Shigella y Clostridium difficile. 4- Estudiar la capacidad de la mezcla mic...

The Foliar Anatomy And Micromorphology Of Aloe Ferox Mill ...

African Journals Online (AJOL)

Background: The genus Aloe has been the subject of several chemotaxonomic and phytochemical investigations. Aloe ferox is an important source of biologically active compounds like anthrones, chromones, pyrones, and flavonoids. It is a plant used extensively in traditional medicine. Despite the myriad of studies on the ...

Monosaccharide analysis of succulent leaf tissue in Aloe

DEFF Research Database (Denmark)

Grace, Olwen Megan; Dzajic, Amra; Jäger, Anna

2013-01-01

in the genus Aloe using a predictive phylogenetic approach. Methodology – Monosaccharide composition was assessed in 31species, representing the morphological and taxonomic diversity of Aloe sensu stricto. Leaf mesophyll polysaccharides were partially hydrolysed in a trifluoroacetic acid (TFA)-SilA assay......Introduction – The succulent leaf mesophyll in Aloe species supports a burgeoning natural products industry, particularly in Africa. Comparative data necessary to prioritise species with economic potential have been lacking. Objective – To survey leaf mesophyll monosaccharide composition....... Oximes and trimethylsilyl ether products were detected by GC-MS. Constituent monosaccharides accounting for the greatest variation among species were identified by principal component analysis. Two plant DNA barcoding regions were sequenced in 28 of the sampled species and the resulting maximum...

Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean.

Science.gov (United States)

Zhang, Fuli; Ge, Honglian; Zhang, Fan; Guo, Ning; Wang, Yucheng; Chen, Long; Ji, Xiue; Li, Chengwei

2016-03-01

Sclerotinia stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary is a major disease of soybean (Glycine max (L.) Merr.). At present, we revealed the three-way interaction between Trichoderma harzianum T-aloe, pathogen S. sclerotiorum and soybean plants in order to demonstrate biocontrol mechanism and evaluate biocontrol potential of T-aloe against S. sclerotiorum in soybean. In our experiments, T-aloe inhibited the growth of S. sclerotiorum with an efficiency of 56.3% in dual culture tests. T-aloe hyphae grew in parallel or intertwined with S. sclerotiorum hyphae and produced hooked contact branches, indicating mycoparasitism. Plate tests showed that T-aloe culture filtrate inhibited S. sclerotiorum growth with an inhibition efficiency of 51.2% and sclerotia production. T-aloe pretreatment showed growth-promoting effect on soybean plants. The activities of peroxidase, superoxide dismutase, and catalase increased, and the hydrogen peroxide (H2O2) as well as the superoxide radical (O2(-)) content in soybean leaves decreased after T-aloe pretreatment in response to S. sclerotiorum pathogen challenge. T-aloe treatment diminished damage caused by pathogen stress on soybean leaf cell membrane, and increased chlorophyll as well as total phenol contents. The defense-related genes PR1, PR2, and PR3 were expressed in the leaves of T-aloe-treated plants. In summary, T-aloe displayed biocontrol potential against S. sclerotiorum. This is the first report of unraveling biocontrol potential of Trichoderma Spp. to soybean sclerotinia stem rot from the three-way interaction between the biocontrol agent, pathogen S. sclerotiorum and soybean plants. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

The Effects of Crude Aqueous and Alcohol Extracts of Aloe Vera on ...

African Journals Online (AJOL)

The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day ...

Aloe vera for prevention and treatment of infusion phlebitis.

Science.gov (United States)

Zheng, Guo Hua; Yang, Liu; Chen, Hai Ying; Chu, Jian Feng; Mei, Lijuan

2014-06-04

Up to 80% of hospitalised patients receive intravenous therapy at some point during their admission. About 20% to 70% of patients receiving intravenous therapy develop phlebitis. Infusion phlebitis has become one of the most common complications in patients with intravenous therapy. However, the effects of routine treatments such as external application of 75% alcohol or 50% to 75% magnesium sulphate (MgSO4) are unsatisfactory. Therefore, there is an urgent need to develop new methods to prevent and alleviate infusion phlebitis. To systematically assess the effects of external application of Aloe vera for the prevention and treatment of infusion phlebitis associated with the presence of an intravenous access device. The Cochrane Peripheral Vascular Diseases Group Trials Search Co-ordinator (TSC) searched the Specialised Register (last searched February 2014) and CENTRAL (2014, Issue 1). In addition the TSC searched MEDLINE to week 5 January 2014, EMBASE to Week 6 2014 and AMED to February 2014. The authors searched the following Chinese databases until 28 February 2014: Chinese BioMedical Database; Traditional Chinese Medical Database System; China National Knowledge Infrastructure; Chinese VIP information; Chinese Medical Current Contents; Chinese Academic Conference Papers Database and Chinese Dissertation Database; and China Medical Academic Conference. Bibliographies of retrieved and relevant publications were searched. There were no restrictions on the basis of date or language of publication. Randomised controlled trials (RCTs) and quasi-randomised controlled trials (qRCTs) were included if they involved participants receiving topical Aloe vera or Aloe vera-derived products at the site of punctured skin, with or without routine treatment at the same site. Two review authors independently extracted the data on the study characteristics, description of methodology and outcomes of the eligible trials, and assessed study quality. Data were analysed using RevMan 5

Development and Operation of the Americas ALOS Data Node

Science.gov (United States)

Arko, S. A.; Marlin, R. H.; La Belle-Hamer, A. L.

2004-12-01

In the spring of 2005, the Japanese Aerospace Exploration Agency (JAXA) will launch the next generation in advanced, remote sensing satellites. The Advanced Land Observing Satellite (ALOS) includes three sensors, two visible imagers and one L-band polarimetric SAR, providing high-quality remote sensing data to the scientific and commercial communities throughout the world. Focusing on remote sensing and scientific pursuits, ALOS will image nearly the entire Earth using all three instruments during its expected three-year lifetime. These data sets offer the potential for data continuation of older satellite missions as well as new products for the growing user community. One of the unique features of the ALOS mission is the data distribution approach. JAXA has created a worldwide cooperative data distribution network. The data nodes are NOAA /ASF representing the Americas ALOS Data Node (AADN), ESA representing the ALOS European and African Node (ADEN), Geoscience Australia representing Oceania and JAXA representing the Asian continent. The AADN is the sole agency responsible for archival, processing and distribution of L0 and L1 products to users in both North and South America. In support of this mission, AADN is currently developing a processing and distribution infrastructure to provide easy access to these data sets. Utilizing a custom, grid-based process controller and media generation system, the overall infrastructure has been designed to provide maximum throughput while requiring a minimum of operator input and maintenance. This paper will present an overview of the ALOS system, details of each sensor's capabilities and of the processing and distribution system being developed by AADN to provide these valuable data sets to users throughout North and South America.

SEBARAN ASPEK KERUANGAN TIPE LONGSORAN DI DAERAH ALIRAN SUNGAI ALO PROVINSI GORONTALO (Spread of Spatial Aspect of Landslide Types at Alo Watershed in Gorontalo Province

Directory of Open Access Journals (Sweden)

Fitryane Lihawa

2015-01-01

Full Text Available ABSTRAK Sebaran aspek keruangan tipe longsoran di DAS Alo Provinsi Gorontalo telah dikaji dan dievaluasi menggunakan bentuk Peta Sebaran Tipe Longsoran Skala 1 : 50.000. Lokasi penelitian ini meliputi seluruh wilayah DAS Alo Provinsi Gorontalo dengan luas 7.588 Ha. Penentuan sampel penelitian dilakukan secara Accidental Sampling yaitu dengan melakukan penelusuran di seluruh wilayah yang rawan longsor di DAS Alo untuk menemukan titik-titik kejadian longsoran. Dalam pengkajian tipe dan sebaran longsoran, dilakukan pengamatan dan pengukuran terhadap kejadian longsoran yang terjadi pada seluruh wilayah DAS Alo yaitu sejumlah 15 (lima belas titik kejadian longsoran.  Tipe longsoran ditentukan melalui pengukuran dan pengamatan morfometri longsoran untuk menentukan indeks klasifikasi longsoran dan hasil analisis tersebut di plot ke dalam Peta Lokasi Sebaran Tipe Longsoran Skala 1 : 50.000 untuk mengetahui sebaran keruangan dari kejadian longsoran di DAS Alo Provinsi Gorontalo. Berdasarkan analisis morfometri dan indeks klasifikasi longsoran menunjukkan bahwa tipe longsoran yang terjadi adalah rotational slide, planar slide, slide flow dan rock block slide. Kejadian longsoran yang terjadi di DAS Alo Provinsi Gorontalo tersebar pada wilayah dengan kemiringan lereng curam dan sangat curam dengan bentuk permukaan lereng cembung dan cenderung lurus. Kejadian longsoran juga terjadi pada wilayah dengan tekstur tanah lempung dan lempung berlanau, serta jenis batuan vulkanik dan batuan beku yang mengandung silika tinggi dan telah mengalami pelapukan. Berdasarkan wilayah  administrasi, kejadian longsoran tersebar di wilayah Kecamatan Tibawa, Kecamatan Pulubala dan Kecamatan Isimu Utara.    ABSTRACT Distribution of spatial aspect of landslide at ALO Watershed of Gorontalo Province has been studied by providing it through form of spread landslide maps at scale of 1 : 50.000. Research site involved all areas of ALO Watershed in Gorontalo Province as having an

Rapid micro-propagation of Aloe vera L. via shoot multiplication

African Journals Online (AJOL)

STORAGESEVER

2008-06-17

. Cloning protocol of Aloe vera as a study-case for tailor- made biotechnology to small farmers. J. Technol. Manag. Innov. 1: 76-79. Debiasi C, Silva CG, Pescador R (2007). Micropropagation of Aloe vera. L., Rev. Bras. PIant.

Effectivity of Aloe vera bioactives as feed additive for broilers reared on deep litter

Directory of Open Access Journals (Sweden)

A.P Sinurat

2004-10-01

Full Text Available There are plenty of Indonesian plants contain usefull bioactive components. One of them is Aloe vera. Previous experiment showed that Aloe vera bioactives reduced aerob bacteria in the intestinal and improved feed efficiency in broilers reared in cages. The results however, gave some variations, may be due to variation in rearing the chickens. Two experiments were carried out to study the effectivity of Aloe vera bioactives as feed additives for broilers reared on deep litter. In the first study, six experimental diets in mash form were formulated, i.e.: Control (K; K + antibiotic; K + 0.50 g/kg dry Aloe vera (LBK; K + 1.00 g/kg LBK; K + anthraquinone; K + Aloe vera in semi-liquid form. The amount of semi liquid Aloe vera and the anthraquinone were equally to 1.00 g LBK/kg. Results showed that antibiotic improved body weight gain 6.10% and feed efficiency 5.50% better than the control, although statistically not significant (P>0.05. Aloe vera bioactives in low doses (0.50 g/kg also improved weight gain (6.30% and feed efficiency (5.20% similar to the antibiotic. However, Aloe vera in high doses and anthraquinone (equal to 1.00 g/kg diet did not improve performance of broilers. There were no significant changes on carcass yield, abdominal fat levels, weight of liver, gizard and gastro intestinal tract due to any feed additives tested. The second experiment were carried out to study the effectivity of feed additives when included in crumble diets. Six experimental diets, i.e.: Control (K, K + antibiotic, K + Semi-liquid Aloe vera (equal to 1.00g dry Aloe vera/kg, K + 0.50 g dry Aloe vera/kg, K + 1.00 g dry Aloe vera/kg, K + 0.50 g dry Aloe vera + 0.50 g Curcuma xanthorrhiza meal/kg. All diets were fed in crumble form. Results showed that chickens fed with feed additives (antibiotic or Aloe vera bioactives have a significantly (P<0.05 higher body weight gain and feed efficiency than those fed with control diet. The best weight gain was achieved by

Impact of goat browsing on Aloe ferox in a South African savanna ...

African Journals Online (AJOL)

The extent and impact of the utilisation of Aloe ferox by Boer goats during winter in a South African savanna was determined using a plant-based approach. All Aloe plants rooted within the transects were eaten by goats, with small plants utilised more frequently than tall plants. The density of dying and dead Aloe plants was ...

Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

Energy Technology Data Exchange (ETDEWEB)

Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Cho, Jae Young; Chung, Byung Yeoup [Chonbuk National University, Jeonju (Korea, Republic of)

2011-10-15

The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, {beta}-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

International Nuclear Information System (INIS)

Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun; Cho, Jae Young; Chung, Byung Yeoup

2011-01-01

The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, β-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

Efficacy of aloe vera gel as an adjuvant treatment of oral submucous fibrosis.

Science.gov (United States)

Alam, Sarwar; Ali, Iqbal; Giri, K Y; Gokkulakrishnan, S; Natu, Subodh S; Faisal, Mohammad; Agarwal, Anshita; Sharma, Himanshu

2013-12-01

Definitive therapy is not defined for the management of oral submucous fibrosis (OSMF). This study evaluated the efficacy of aloe vera gel as an adjuvant treatment of OSMF. A double-blind, placebo-controlled, parallel-group randomized controlled trial was conducted on 60 subjects with OSMF divided into medicinal treatment (submucosal injection of hyaluronidase and dexamethasone, n = 30) and surgical treatment (n = 30) categories. Each category was randomly divided into groups A (with aloe vera, n = 15 per category) and B (without aloe vera, n = 15 per category). Follow-up assessment for various symptoms was performed, and results were analyzed using paired and unpaired Student t tests. The group receiving aloe vera had a significant improvement in most symptoms of OSMF (P aloe vera group, in both the medicinal and surgical categories. Aloe vera gel was effective as an adjuvant in treatment of OSMF. Copyright © 2013 Elsevier Inc. All rights reserved.

Aloe Gel Enhances Angiogenesis in Healing of Diabetic Wound

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Djanggan Sargowo

2011-12-01

Full Text Available BACKGROUND: Diabetic micro and macroangiophathy lead to the incident of diabetic foot ulcers characterized by an increased number of circulating endothelial cells (CECs and decreased function of endothelial progenitor cells (EPCs. This fact is correlated with ischemia and diabetic wound healing failure. Aloe vera gel is known to be able to stimulate vascular endothelial growth factor (VEGF expression and activity by enhancing nitric oxide (NO production as a result of nitric oxide synthase (NOS enzyme activity. Aloe vera is a potential target to enhancing angiogenesis in wound healing. OBJECTIVE: The objective of this study was to explore the major role of Aloe vera gel in wound healing of diabetic ulcers by increasing the level of EPCs, VEGF, and endothelial nitric oxide synthase (eNOS, as well as by reducing the level of CECs involved in angiogenesis process of diabetic ulcers healing. METHODS: The experimental groups was divided into five subgroups consisting of non diabetic wistar rats, diabetic rats without oral administration of aloe gel, and treatment subgroup (diabetic rats with 30, 60 and 120 mg/day of aloe gel doses for 14 days. All subgroups were wounded and daily observation was done on the wounds areas. Measurement of the number of EPCs (CD34, and CECs (CD45 and CD146 was done by flow cytometry, followed by measurement of VEGF and eNOS expression on dermal tissue by immunohistochemical method on day 0 and day 14 after treatment. The quantitative data were analyzed by One-Way ANOVA and Linear Regression, with a confidence interval 5% and significance level (p<0.05 using SPSS 16 software to compare the difference and correlation between wound diameters, number of EPCs and CECs as well as the levels of VEGF and eNOS. RESULTS: The results of this study showed that aloe gel oral treatment in diabetic wistar rats was able to accelerate the wound healing process. It was shown by significant reduction of wound diameter (0.27±0.02; the

LA GUÍA ESTRATÉGICA EL CORAZÓN DEL PLAN ESTRATÉGICO

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Juan Pablo Lema

Full Text Available La misión, la visión y los valores no son el plan estratégico de una compañía. Son el eje del plan estratégico y, por ello, se consideran su guía o corazón. Su enunciación no es un proceso aislado que surge de la nada, sino que constituye una etapa del complejo proceso de planeación estratégica, que debe alimentarse del análisis de investigaciones del entorno y de la empresa y enmarcarse dentro de unos posibles escenarios de la realidad futura. Igualmente, soporta la realización concreta de las estrategias, los objetivos y las acciones que ayudan a materializarlos y volver tangible, operativa, controlable y mensurable la estrategia del negocio.

Comparative study of antimicrobial activities of Aloe vera extracts ...

African Journals Online (AJOL)

Plants are of relevance to dermatology for both their beneficial and adverse effects on skin and skin disorders. One of the medicinal plants, Aloe vera (Aloe barbadensis Miller), is reputed to have medicinal properties. For centuries, it has been used for an array of ailments such as mild fever, wounds and burns, ...

Aprendizaje centrado en las perspectivas del paciente: el caso de las escuelas de medicina en México

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Silvia Lizett Olivares Olivares

2017-01-01

Discusión: El modelo presentado evoluciona desde la educación basada en competencias implementadas en la educación a partir de contenidos aislados, a un enfoque en base a perspectivas que colocan en el centro al paciente para abordar desde un mismo caso múltiples competencias del médico.

Karakterisasi Erwinia Chrysanthemi Penyebab Penyakit Busuk Bakteri Pada Daun Lidah Buaya (Aloe Vera)

OpenAIRE

SUPRIADI,; IBRAHIM, NILDAR; TARYONO,

2002-01-01

Characteristics o/Envinia chysanthemi causing bacterial soft rot ofAloe (Aloe VeraJThe bacterial sot rot of aloe, caused by Erwinia chrysanthemi, was first identified in Caibbean Island in 1992. In early 2001, similar symptoms were found on the aloe plants grown in Semplak, Bogor, West Java. Based on its symptom and progressively spread, especially on the leaf and basal stem, it appeared that the disease was serious and therefore threatened the current development of die plants. This study wa...

Aislamiento y caracterización de microorganismos con actividad lipolítica provenientes de sedimentos del Humedal El Jaboque (Bogotá, Colombia

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Lilia Carolina Mora Moreno

2006-07-01

sedimentos, mediante la utilización de medios de cultivo selectivos (agar tributirina, agar aceite de girasol. Primero se evaluó la concentración del sustrato (aceite de girasol en la cual se encontraba el mayor número de morfotipos aislados, luego se comparó la eficiencia de los dos medios de cultivo de acuerdo a la cantidad de morfotipos aislados. Se evaluaron tres concentraciones diferentes de sustrato. Se aisló un total de 18 cepas pertenecientes a los géneros Bacillus, Pseudomonas, Acinetobacter, Streptomyces, Penicillium, Alternaria. Los medios evaluados constituyen alternativas sencillas de aislamiento de microorganismos con actividad lipolítica en sedimentos.

Actividad biológica de la prostaglandina F2 alfa y del metil carboprost en el fundus gástrico aislado de rata

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Roberto Rojas Martínez

1997-08-01

Full Text Available Se comparó la potencia contráctil de una muestra del éster metílico de 15 metil prostaglandina F2 alfa (metil carboprost (I, producida por la Industria Farmacéutica Cubana, con una muestra comercial de prostaglandina F2 alfa (II, de la firma Chinoin, mediante el ensayo de los "tres puntos". También se comparó la duración de la contracción inducida por dichas muestras utilizando un análisis de regresión lineal de la relajación observada en función del tiempo, luego de suprimir el contacto entre la droga y el órgano efector. Se utilizó el fundus gástrico aislado de rata como órgano efector. La actividad contráctil se registró mediante transductores fuerza-desplazamiento. La muestra I presentó una potencia relativa de 164,0 % con respecto a la II, con límites de confianza de 111,2 y 247,8 % (p=0,95; N=7, y mostró un efecto más prolongado en relación con la II. En las tiras contraídas por la muestra I debió transcurrir 6 min y 46 s, luego del lavado, para que la contracción se redujera a la mitad de la respuesta máxima observada (N=7, en tanto que en las tiras contraídas por la II fue de 1 min y 48 s (N= 8.The contractil potency of a sample of methylic ester of 15 methyl prostaglandin F2 alpha (methyl carboprost (I produced by the Cuban Pharmaceutical Industry, was compared to a commercial sample of prostaglandin F2 alpha (II from a Chinoin firm through the "three point" assay. The duration of the contraction induced by such samples was also compared using the analysis of linear regression of the relaxation observed in function of time after the supression of contact between the drug and the effector organ. The gastric fundus isolated from a rat was used as the effector organ. The contractil activity was recorded by strength-displacement transducers. Sample I showed a relative potency of 164.0 % with respect to sample II, with confidence limits of 111.2 and 247.8 % (P = 0.95; N=7 and it also showed a more prolonged

Toxicology and carcinogenesis studies of a nondecolorized [corrected] whole leaf extract of Aloe barbadensis Miller (Aloe vera) in F344/N rats and B6C3F1 mice (drinking water study).

Science.gov (United States)

Boudreau, M D; Beland, F A; Nichols, J A; Pogribna, M

2013-08-01

Extracts from the leaves of the Aloe vera plant (Aloe barbadensis Miller) have long been used as herbal remedies and are also now promoted as a dietary supplement, in liquid tonics, powders or tablets, as a laxative and to prevent a variety of illnesses. We studied the effects of Aloe vera extract on rats and mice to identify potential toxic or cancer-related hazards. We gave solutions of nondecolorized extracts of Aloe vera leaves in the drinking water to groups of rats and mice for 2 years. Groups of 48 rats received solutions containing 0.5%, 1% or 1.5% of Aloe vera extract in the drinking water, and groups of mice received solutions containing 1%, 2%, or 3% of Aloe vera extract. Similar groups of animals were given plain drinking water and served as the control groups. At the end of the study tissues from more than 40 sites were examined for every animal. In all groups of rats and mice receiving the Aloe vera extract, the rates of hyperplasia in the large intestine were markedly increased compared to the control animals. There were also increases in hyperplasia in the small intestine in rats receiving the Aloe vera extract, increases in hyperplasia of the stomach in male and female rats and female mice receiving the Aloe vera extract, and increases in hyperplasia of the mesenteric lymph nodes in male and female rats and male mice receiving the Aloe vera extract. In addition, cancers of the large intestine occurred in male and female rats given the Aloe vera extract, though none had been seen in the control groups of rats for this and other studies at this laboratory. We conclude that nondecolorized Aloe vera caused cancers of the large intestine in male and female rats and also caused hyperplasia of the large intestine, small intestine, stomach, and lymph nodes in male and female rats. Aloe vera extract also caused hyperplasia of the large intestine in male and female mice and hyperplasia of the mesenteric lymph node in male mice and hyperplasia of the stomach

THE EFFECT OF ALOE VERA ON GASTRIC ACID SECRETION ...

African Journals Online (AJOL)

The effect of varying doses of ethanol extract of Aloe vera (Liliaceae) on acute gastric mucosal lesions induced by 0.6M HCl and acid output was studied in the pylorus ligated and lumen perfuse rats respectively. Acid secretion was determined by titration of the collected gastric juice to pH 7.0. Intraperitoneal injection of Aloe ...

A revised generic classification for Aloe (Xanthorrhoeaceae subfam. Asphodeloideae)

DEFF Research Database (Denmark)

Grace, Olwen Megan; Klopper, Ronell R.; Smith, Gideon F.

2013-01-01

The predominantly southern African Xanthorrhoeaceae subfam. Asphodeloideae Asphodelaceae subfam. Alooideae) has long been regarded as comprising seven so-called alooid genera (Aloe, Astroloba, Chortolirion, Gasteria, Haworthia, Lomatophyllum, Poellnitzia). A reassessment of the classification...... Kumara is reinstated and the new genera Aloidendron and Aloiampelos are established. New combinations are made in Aloe for the three species of Chortolirion....

Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.

Science.gov (United States)

Tummalapalli, Mythili; Berthet, Morgane; Verrier, Bernard; Deopura, B L; Alam, M S; Gupta, Bhuvanesh

2016-01-01

Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management. Copyright © 2015 Elsevier B.V. All rights reserved.

Analyses of Aloe polysaccharides using carbohydrate microarray profiling

DEFF Research Database (Denmark)

Isager Ahl, Louise; Grace, Olwen M; Pedersen, Henriette Lodberg

2018-01-01

As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another and the effects they have on the human body are increasing. Polysaccharides present in the leaf mesophyll have been...... identified as the components responsible for the biological activities of Aloe vera, and they have been widely studied in the past decades. However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions as most of them require...... a complete or near-complete fractionation of the polymers. The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in Aloe leaf mesophyll. The method we chose is known as Comprehensive Microarray Polymer Profiling...

Effect of Aloe vera (Aloe barbadensis Miller) on survivability, extent of proteolysis and ACE inhibition of potential probiotic cultures in fermented milk.

Science.gov (United States)

Basannavar, Santosh; Pothuraju, Ramesh; Sharma, Raj Kumar

2014-10-01

In the present investigation, the effect of Aloe vera gel powder on angiotensin-converting enzyme (ACE) inhibitory activity, extent of proteolysis during fermentation and survival of Lactobacillus casei NCDC19 during storage of fermented milk was studied. Among the different cultures screened for ACE inhibitory activity, Lactobacillus casei NCDC 19 exhibited the highest ACE inhibition (approx. 40%) as well as extent of proteolysis (0.37, Abs₃₄₀). In the presence of Aloe vera (0.5% and 1% w/v) an increase in extent of proteolysis (0.460 ± 0.047 and 0.480 ± 0.027) and percent ACE inhibitory activity (44.32 ± 2.83 and 47.52 ± 1.83) was observed in comparison to control. Aloe vera powder addition also led to an increase in viable counts (>11 log cfu mL⁻¹) of L. casei NCDC 19 in fermented milk during storage for 7 days and the counts were maintained in sufficiently higher numbers. The study suggests Aloe vera to be a good functional ingredient which can be further explored for different health attributes. © 2014 Society of Chemical Industry.

The effect of aloe vera on ischemia--Reperfusion injury of sciatic nerve in rats.

Science.gov (United States)

Guven, Mustafa; Gölge, Umut Hatay; Aslan, Esra; Sehitoglu, Muserref Hilal; Aras, Adem Bozkurt; Akman, Tarik; Cosar, Murat

2016-04-01

Aloe vera is compound which has strong antioxidant and anti-inflammatory effects. We investigated the neuroprotective role of aloe vera treatment in rats with experimental sciatic nerve ischemia/reperfusion injury. Twenty-eight male Wistar Albino rats were divided equally into 4 groups. Groups; Control group (no surgical procedure or medication), sciatic nerve ischemia/reperfusion group, sciatic nerve ischemia/reperfusion+aloe vera group and sciatic nerve ischemia/reperfusion+methylprednisolone group. Ischemia was performed by clamping the infrarenal abdominal aorta. 24 hours after ischemia, all animals were sacrificed. Sciatic nerve tissues were also examined histopathologically and biochemically. Ischemic fiber degeneration significantly decreased in the pre-treated with aloe vera and treated with methylprednisolone groups, especially in the pre-treated with aloe vera group, compared to the sciatic nerve ischemia/reperfusion group (paloe vera group was not statistically different compared to the MP group (p>0.05). Aloe vera is effective neuroprotective against sciatic nerve ischemia/reperfusion injury via antioxidant and anti-inflammatory properties. Also aloe vera was found to be as effective as MP. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Caracterización molecular de la región determinante de resistencia a quinolonas (QRDR de la topoisomerasa IV de Bartonella bacilliformis en aislados clínicos

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Abraham Espinoza-Culupú

2014-05-01

Full Text Available Bartonella bacilliformis es el agente etiológico de la Enfermedad de Carrión, endémica del Perú. Pocas investigaciones han sido realizadas acerca de los genes asociados a la resistencia antimicrobiana en aislados clínicos de este patógeno. Estos genes no están caracterizados molecularmente, ni se conoce la región asociada a dicha resistencia. Por ello, el objetivo del este trabajo fue caracterizar molecularmente la región determinante de la resistencia a las quinolonas (QRDR en la topoisomerasa IV, que está codificada por los genes parC y parE, así como también desarrollar una prueba de susceptibilidad antimicrobiana para B. bacilliformis. Las muestras sanguíneas de 65 pacientes procedentes de La Libertad, Cusco, Ancash y Piura, se sembraron en placas de agar sangre e incubaron a 30 °C con 5% CO2. Luego se procedió a: (1 determinar la susceptibilidad antimicrobiana y (2 extraer el DNA genómico, amplificar los genes mencionados, secuenciarlos y analizarlos mediante herramientas bioinformáticas. Se obtuvieron 6 cultivos positivos. Los aislados fueron sensibles a la ciprofloxacina (excepto uno procedente de Quillabamba-Cusco, que presentó susceptibilidad disminuida y resistentes al ácido nalidíxico. Del análisis de las secuencias aminoacídicas de ParC y ParE de B. bacilliformis se concluye que presentan diferencias aminoacídicas en comparación con las secuencias de las proteínas respectivas de E. coli K12 MG1655, que probablemente confieran resistencia al ácido nalidíxico pero no a la ciprofloxacina. Se determinó que las QRDR de las proteínas ParC y ParE de B. bacilliformis están comprendidas entre los aminoácidos 67 al 118 y 473 al 530, respectivamente. El antibiograma y la concentración mínima inhibitoria se evalúan mejor usando inóculos a escala 1 de McFarland y a los 6 días de incubación.

Streptococcus pneumoniae aislados durante 2002-2006: serotipos y resistencia antibiótica. Correlación con las vacunas existentes

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José María Guevara-Duncan

2008-03-01

Full Text Available Objetivos: Identificar los serotipos de S. pneumoniae aislados, correlacionándolos con los incluidos en las vacunas existentes y su resistencia antimicrobiana. Diseño: Estudio descriptivo, observacional y longitudinal. Lugar: Instituto de Medicina Tropical Daniel A. Carrión, Facultad de Medicina, UNMSM. Material biológico: Cepas de Streptococcus pneumoniae. Intervenciones: Cuarenta Streptococcus pneumoniae de nuestro cepario, aislados entre el 2002 y 2006, fueron serotipificados en el Instituto de Salud Carlos III en Madrid -España; 15 fueron invasivos, 11 aislados de infecciones localizadas, 6 de portadores y 8 eran multiresistentes. Principales medidas de resultados: Protección de las vacunas existentes en nuestro medio a las infecciones causadas por Streptococcus pneumoniae. Resultados: Hubo 14 serotipos diferentes y los serogrupos más identificados fueron 23, 19 y 6. El 28,6% estaba contenido en la vacuna 7-valente, 42,9% en la 9-valente, 50% en la 11-valente y el 71,4% en la 23-valente; 57,5% fue resistente a la penicilina y 30% a eritromicina. El grupo de Streptococcus invasivo resultó más sensible a los antibióticos que los otros grupos. Los serotipos asociados a multirresistencia fueron 19F y 23F. Conclusiones: Ninguna de las vacunas protege a todas las infecciones causadas por Streptococcus pneumoniae, en nuestro medio.

Fast Dissolving Tablets of Aloe Vera Gel | Madan | Tropical Journal ...

African Journals Online (AJOL)

Purpose: The objective of this work was to prepare and evaluate fast dissolving tablets of the nutraceutical, freeze dried Aloe vera gel. Methods: Fast dissolving tablets of the nutraceutical, freeze-dried Aloe vera gel, were prepared by dry granulation method. The tablets were evaluated for crushing strength, disintegration ...

Evaluación de la actividad desulfurizadora de aislados nativos de Pseudomonas spp. en presencia de hidrocarburo Desulfurization activity evaluation of native strains of Pseudomonas spp. in the presence of hydrocarbon

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Alméciga-Díaz Carlos Javier

2005-07-01

Full Text Available El principal inconveniente en la combustión de los hidrocarburos es la conversión del azufre y el nitrógeno a sus respectivos óxidos, los cuales participan en la formación de lluvia acida y deterioran el medio ambiente e infraestructuras. La remoción de azufre a partir de compuestos órgano-azufrados mediante el uso de microorganismos ha surgido como una alternativa frente al proceso catalítico de hidrodesulfurización (HDS. En el presente trabajo se evaluó la actividad desulfurizadora de veintitrés aislados nativos de Pseudomonas spp. sobre dibenzotiofeno (DBT, usando un sistema de fermentación con igual proporción de fase acuosa y orgánica (n-hexano en presencia de oleato de etanolamina. Los aislados 02,05 y 06 conservaron su viabilidad en este medio y presentaron una remoción de azufre entre 6,0 y 9,4%, generando los metabolitos DBT-sulfona, DBT-sulfóxido, 2-hidroxibifenilo (2-HBP y sulfato presentes en la ruta metabólica 4S. Con estos aislados se evaluó la actividad desulfurizadora sobre keroseno y se observó una remoción de azufre entre 19,9 y 62,6% y una disminución del poder calorífico entre 0,45 y 5,55%. Palabras clave: dibenzotiofeno, desulfurización, Pseudomonas spp., keroseno.The main difficulty with fossil fuel combustión lies in sulphur and nitrogen becoming converted to their respective oxides, forming part of the acid rain which deteriorates the environment and infrastructure. Removing sulphur from organo-sulfur compounds by using micro-organisms has become an alternative to hydrodesulphurisation (HDS. Twenty-three Pseudomonas spp. native strains' desulphurisation activity on dibenzothiophene (DBT was evaluated by using a fermentation system having equal proportions of aqueous and organic (n-hexane phases in the presence of ethanolamine oléate. The 02, 05 and 06 strains maintained their viability in this médium, presenting 6,0% to 9,4% sulphur removal, producing DBT-sulphone, DBT-sulphoxide, 2

Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp

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Alexander Pérez Cordero

2011-04-01

Full Text Available Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo del género Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D. rotundata en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojas de M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, el método de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobre seis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 y BVC 507. Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del medio papa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 µl de cada extracto por separado. Se utilizó un control positivo con nistatina (4 mg/ml y un testigo absoluto sin ningún tipo de tratamiento. La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96 y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probar diferencias significativas entre tratamientos, cepas (aislados y tiempo de inhibición. Los resultados expresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de las hojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similar al control positivo.The objective of this study was to evaluate in vitro inhibitory activity of extracts from leaves of Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp. Melia azederach and Mascagnia concinna on isolated fungus of the genus Colletotrichum, which causes anthracnose disease in the crop of yam (Dioscorea alata, D. rotundata in the department of Sucre, Colombia. To obtain

Symbiotics and Aloe vera and Symphytum officinale extracts in broiler feed

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Paula Rodrigues Oliveira

2016-09-01

Full Text Available This study aimed to test the effects of dietary Aloe vera and Symphytum officinale extracts added separately or in combination with symbiotics on the performance, nutrient utilization, serum biochemical parameters, biometrics, and intestinal histomorfometry of broilers. The experiment had a randomized block design with five treatments and six replicates of ten broilers each. Treatments were as follows: negative control and positive control (diet without and with antibiotic, respectively; 0.2% Aloe vera (AV; 0.2% Symphytum officinale (S; 0.2% functional supplement, composed of symbiotics fermented in Aloe vera and comfrey plant extracts (S+PE. At seven days of age, FI of birds fed the Aloe vera extracts diets were lower than that observed for birds consuming the diet with Symphytum officinale extract and S+PE. Broiler performance remained unaffected by treatments at others ages evaluated. At 10 to 14 days of age the lowest ADCDM ADCCP was shown in group feed NC. The highest ADCCP was observed in PC control group and in diets supplemented with Aloe vera and S+PE. Serum levels of cholesterol, triglycerides, and phosphorus were affected by addition of extracts at seven, 21, and 35 days of age. The longest duodenal villi were observed in broilers fed S+PE diets at seven days of age. Aloe vera and Symphytum officinale extracts and symbiotics can be used in broiler diets as an alternative to growth-promoting antibiotics.

Stimulation of osteoblast activity by induction of Aloe vera and xenograft combination

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Utari Kresnoadi

2011-12-01

Full Text Available Background: Tooth extraction is generally followed by alveolar ridge resorption that later can cause flat ridge. Aloe vera have biogenic stimulator and hormone activities for wound healing. Purpose: This study was aimed to know osteoblast activities in alveolar bone after induction of Aloe vera and XCB combination. Methods: Fifty four of Cavia cabaya were divided into three main groups. Group I was control group. Group II was filled with xenograft concelous bovine (XCB and group III was filled with the combination of Aloe vera gel and XCB. Then, each group was divided into three sub groups according to timing, they are 14, 30, and 60 days after tooth extraction and application. Histology and morphology examination were performed on the harvested specimens. Results: There were significant differences between the control group and the other groups filled with the combination of Aloe vera and XCB. Conclusion: In conclusion, the application of Aloe vera gel and xenograft combination decrease the number of osteoclast and increase the number of osteoblast in post tooth extraction alveolar bone structure indicating the new growth of alveolar bone.Latar belakang: Pencabutan gigi pada umumnya selalu diikuti resopsi tulang alveolar, sehingga bila terjadi dalam waktu yang lama ridge akan menjadi flat. Aloe vera adalah bahan stimulasi biogenik dan mempunyai aktivitas hormon untuk proses penyembuhan luka. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui aktivitas osteoblas pada tulang alveol dengan pemberian kombinasi Aloe vera gel dan xenograft concelous bovine (XCB. Metode: Lima puluh empat ekor Cavia cabaya, dibagi menjadi 3 kelompok besar, kelompok pertama adalah kelompok kontrol yaitu hanya dilakukan pencabutan saja tanpa perlakuan, kelompok ke-2 yaitu kelompok yang setelah dicabut diberi XCB saja dan kelompok ke-3 yaitu kelompok yang setelah pencabutan diberi kombinasi Aloe vera gel dengan XCB pada luka bekas pencabutan gigi. Kemudian masing

Clear Evidence of Carcinogenic Activity by a Whole-Leaf Extract of Aloe barbadensis Miller (Aloe vera) in F344/N Rats

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Boudreau, Mary D.

2013-01-01

Aloe barbadensis Miller (Aloe vera) is an herbal remedy promoted to treat a variety of illnesses; however, only limited data are available on the safety of this dietary supplement. Drinking water exposure of F344/N rats and B6C3F1 mice to an Aloe vera whole-leaf extract (1, 2, and 3%) for 13 weeks resulted in goblet cell hyperplasia of the large intestine in both species. Based upon this observation, 2-year drinking water studies were conducted to assess the carcinogenic potential of an Aloe vera whole-leaf extract when administered to F344/N rats (48 per sex per group) at 0.5, 1, and 1.5%, and B6C3F1 mice (48 per sex per group) at 1, 2, and 3%. Compared with controls, survival was decreased in the 1.5% dose group of female rats. Treatment-related neoplasms and nonneoplastic lesions in both species were confined primarily to the large intestine. Incidences of adenomas and/or carcinomas of the ileo-cecal and cecal-colic junction, cecum, and ascending and transverse colon were significantly higher than controls in male and female rats in the 1 and 1.5% dose groups. There were no neoplasms of the large intestine in mice or in the 0 or 0.5% dose groups of rats. Increased incidences of mucosa hyperplasia of the large intestine were observed in F344/N rats, and increased incidences of goblet cell hyperplasia of the large intestine occurred in B6C3F1 mice. These results indicate that Aloe vera whole-leaf extract is an intestinal irritant in F344/N rats and B6C3F1 mice and a carcinogen of the large intestine in F344/N rats. PMID:22968693

Perfil microbiológico y sensibilidad a antibióticos de microorganismos aislados de infecciones conjuntivales en el Instituto de Oftalmología Fundación Conde de Valenciana. Reporte del año 2012

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Patricia Chirinos-Saldaña

2014-04-01

Conclusiones: Staphylococcus epidermidis fue el microorganismo aislado con mayor frecuencia en muestras provenientes de infecciones conjuntivales. Todas las cepas de S. epidermidis fueron sensibles a vancomicina, gentamicina y moxifloxacino y la mayoría de ellas fueron multirresistentes a los antibióticos en evaluación.

La infección del tracto urinario (ITU en un hospital universitario de Santafé de Bogotá: etiología y evaluación de los antimicrobianos

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S. Máttar

1994-09-01

Full Text Available Los objetivos de este trabajo fueron determinar la etiología y la sensibilidad antimicrobiana en la infección del tracto urinario (ITU. Para ello, se analizaron prospectivamente 245 orinas extra e intrahospitalarias del Hospital Universitario San Ignacio de Santafé de Bogotá. Las orinas se estudiaron a través del sedimento, Gram, tira reactiva y cultivo bacteriológico; la susceptibilidad antimicrobiana se realizó por el método de Kirby Bauer. De las 245 orinas se descartaron 45; las 200 orinas restantes fueron cultivadas, resultando 100 positivas y 100 negativas. El agente más frecuentemente aislado fue E. coli (85%, seguido de Proteus sp. (6%. Los microorganismos aislados presentaron una alta sensibilidad a la cefotaxima (100%, norflocacina (98%, netilmicina (98%, amikacina (98%, nitrofurantoína (92%, gentamicina (86%, ácido nalidixico (78% y una sensibilidad del 57% frente al trimetoprim-sulfametoxasol y el 25% a la ampicilina. Los antimicrobianos eficaces para el tratamiento de la ITU, por su bajo costo y alta actividad sobre los microorganismos encontrados, son: nitrofurantoina, norfloxacina, amikacina, netilmicina, gentamicina y ácido nalidíxico.

Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review

Science.gov (United States)

Radha, Maharjan H.; Laxmipriya, Nampoothiri P.

2014-01-01

Aloe vera (蘆薈 lú huì) is well known for its considerable medicinal properties. This plant is one of the richest natural sources of health for human beings coming. The chemistry of the plant has revealed the presence of more than 200 different biologically active substances. Many biological properties associated with Aloe species are contributed by inner gel of the leaves. Most research has been centralized on the biological activities of the various species of Aloe, which include antibacterial and antimicrobial activities of the nonvolatile constituents of the leaf gel. Aloe species are widely distributed in the African and the eastern European continents, and are spread almost throughout the world. The genus Aloe has more than 400 species but few, such as A. vera, Aloe ferox, and Aloe arborescens, are globally used for trade. A. vera has various medicinal properties such as antitumor, antiarthritic, antirheumatoid, anticancer, and antidiabetic properties. In addition, A. vera has also been promoted for constipation, gastrointestinal disorders, and for immune system deficiencies. However, not much convincing information is available on properties of the gel. The present review focuses on the detailed composition of Aloe gel, its various phytocomponents having various biological properties that help to improve health and prevent disease conditions. PMID:26151005

Caracterización molecular y agronómica de aislados de Trichoderma spp nativos del noreste de México

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José Luis Hernández Mendoza

2011-07-01

Full Text Available Título en ingles: Molecular and agronomic characterization of Trichoderma spp natives of northeastern Mexico  Resumen Trichoderma sp es un hongo frecuentemente usado en actividades agrícolas, pues actúa como antagonista de diversas especies de hongos fitopatógenos. En este estudio se realizó el aislamiento de cuatro cepas de Trichoderma sp nativas del noreste de México, las cuales fueron identificadas a nivel molecular mediante la secuenciación del ITS 1. Además se evaluó su capacidad antagonista en contra los hongos fitopatógenos Macrophomina phaseolina y Fusarium oxysporum, que afectan severamente cultivos de sorgo, maíz y fríjol en el noreste de México. La identificación se realizó de acuerdo al grado de concordancia con secuencias reportadas y corresponden a las especies T. hammatum (HK701;  T. koningiopsis (HK702; T. asperellum (HK703 y Trichoderma sp (HK704. Por otra parte, las pruebas de antagonismo muestran que los aislados HK701, HK703 y HK704 inhiben por competencia el crecimiento de M. phaseolina y F. oxysporum, mientras que HK702 tiene la capacidad para hiperparasitar dichos fitopatógenos. Finalmente, se evaluó la promoción de crecimiento de T. asperellum HK703, en maíz (Pionner 30P49®, usando para ello concentraciones de tratamiento de 1x10e2 hasta 1x10e6 esp/mL. En estos ensayos se midió la producción de biomasa. Los resultados muestran que en concentraciones intermedias se tiene el mayor incremento en altura de plantas y mayor producción de peso seco en follaje y raíz. Entre los parámetros antes mencionados existen diferencias significativas. Palabras clave: control biológico, antagonismo, biofertilizantes, Zea maiz, Sorghum bicolor. Abstract Trichoderma sp is a fungus often used in agricultural activities, because it acts as an antagonist of several species of plant pathogenic fungi. In this study four strains of Trichoderma sp was isolated from the northeastern Mexico, which were identified by

Evaluación del potencial biofertilizante de bacterias diazótrofas aisladas de suelos con cultivo de cacao (Theobroma cacao L.

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Adriana Zulay Argüello-Navarro

2014-09-01

Full Text Available La adaptabilidad del cacao (Theobroma cacao L. en condiciones adversas permite sugerir que la microbiota asociada con la rizosfera desempeña una función importante para la nutrición y el desarrollo de la planta. En el trabajo se evaluó el potencial biofertilizante de bacterias fijadoras de nitrógeno (diazótrofas aisladas de suelos cacaoteros por la técnica de diluciones seriadas. Las bacterias se aislaron en medios semiselectivos (NFb, JMV, LGI, JNFb. Los aislados puros se caracterizaron morfológica y bioquímicamente y complementariamente se evaluó la capacidad de fijación biológica de nitrógeno (FBN por el método de Micro-Kjeldahl. Los aislados con mejor capacidad de fijación de nitrógeno fueron seleccionados para evaluar su potencial promotor de crecimiento durante 120 días en plantas de cacao clon CCN 51 establecidas a partir de semillas. El análisis de datos mostró que las plantas de cacao respondieron efectivamente a los tratamientos con bacterias donde las variables de crecimiento y N (% foliar incrementaron significativamente, en comparación con el testigo. Se destacó el aporte de los aislados DKA2J24, DKB3J73 presuntamente del género Burkholderia sp. y DKA1L3 semejante al género Gluconacetobacter sp., todos con un gran potencial como biofertilizantes en la prueba de bioensayo.

Arquitectura contemporánea en rollizo en las regiones del Bío-Bío y La Araucanía

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Rodrigo Villalobos P.

1997-05-01

Full Text Available En la regiones del Bío-Bío y de la Araucanía se ha observado en el transcurso de los últimos años, un notorio desarrollo de arquitectura en rollizo de pino. Hasta ahora la historia arquitectónica del sur del país no había mostrado un masivo empleo del rollizo y los ejemplos existentes eran más bien aislados, como el caso del Fuerte Bulnes en la Patagonia Austral o la arquitectura tradicional mapuche en que los troncos de madera fueron utilizados como estructura. A diferencia de los Estados Unidos y Canadá, en que apareció arquitectura en rollizo desde los tiempos de la conquista del Oeste.

Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study

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Rezazadeh, Fahimeh; Moshaverinia, Maryam; Motamedifar, Mohammad; Alyaseri, Montazer

2016-01-01

Statement of the Problem Herpes simplex virus (HSV) infection is one of the most common and debilitating oral diseases; yet, there is no standard topical treatment to control it. The extract of Aloe vera leaves has been previously reported to have anti-inflammatory, antibacterial, and also antiviral effects. There is no data on anti-Herpes simplex virus type 1 (HSV-1) activity of Aloe vera gel. Purpose This study aimed to evaluate the anti-HSV-1 activity of Aloe vera gel in Vero cell line. Materials and Method In this study, gel extraction and cytotoxicity of various increasing concentrations of Aloe vera gel (0.2, 0.5, 1, 2, and 5%) was evaluated in Dulbecco’s Modified Eagle Medium (DMEM) containing 2% fetal bovine serum (FBS). Having been washed with phosphate buffered saline, 50 plaque-forming units (PFU) of HSV-1 was added to each well. After 1 hour of incubation at 37°C, cell monolayers in 24 well plates were exposed to different increasing concentrations of Aloe vera gel. The anti-HSV-1 activity of Aloe vera gel in different concentrations was assessed by plaque reduction assays. Data were analyzed by using One-way ANOVA. Results The cytotoxicity assay showed that Aloe vera in prearranged concentrations was cell-compatible. The inhibitory effect of various concentrations of Aloe vera was observed one hour after the Vero cell was infected with HSV-1. However, there was no significant difference between two serial concentrations (p> 0.05). One-way ANOVA also revealed no significant difference between the groups. The findings indicated a dose-dependent antiviral effect of Aloe vera. Conclusion The findings showed significant inhibitory effect of 0.2-5% Aloe vera gel on HSV-1 growth in Vero cell line. Therefore, this gel could be a useful topical treatment for oral HSV-1 infections without any significant toxicity. PMID:26966709

Efecto de sedimentos naturales enriquecidos con zinc, en modelos aislados y en microcosmos, sobre tres especies de invertebrados bentónicos

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Marcela Galar Martínez

2006-06-01

Full Text Available En los cuerpos acuáticos la disponibilidad de los tóxicos es limitada por las características fisicoquímicas de los sedimentos y agua, así como por las interacciones entre los diversos xenobióticos y entre las especies que los habitan. Por lo anterior, el objetivo del presente trabajo fue relacionar el efecto producido por el zinc (Zn sembrado en sedimentos del embalse Ignacio Ramírez (PIR, en modelos aislados y en microcosmos, sobre el ATP de tres organismos bentónicos con la biodisponibilidad del metal. Las especies seleccionadas fueron: Hyalella azteca, Limnodrilus hoffmeisteri y Stagnicola attenuata, dado que se encuentran en altas proporciones en el embalse y ocupan espacios diferentes en el bentos. Las muestras de sedimentos y organismos se colectaron en la estación de sequía (febrero de 1999. A los primeros se les determinó el pH, textura, tamaño de partícula, nitrógeno total, materia orgánica y concentración de metales (Zn, Fe, Cu y Ni. Posteriormente se realizó un estudio de toxicidad subletal utilizando dos tipos de sistemas estáticos (organismos aislados y en microcosmos. Ambos modelos contenían sedimentos de la PIR enriquecidos con Zn (concentración nominal de 0.8129 mg/kg y agua sintética en una proporción de 1:4. Los organismos de prueba fueron adicionados una vez alcanzado el equilibrio (2 hr considerando la cantidad de biomasa con respecto al volumen (1.0 g de organismo por cada 100 ml de agua:sedimento. Después de 0, 12, 24, 36, 48 y 72 hr de exposición se tomaron muestras del sedimento y de los hidrobiontes, a los cuales se les cuantificó el contenido de Zn por absorción atómica. A los organismos también se les determinó la concentración de ATP. Los resultados obtenidos muestran que el efecto producido por los sedimentos naturales enriquecidos se ve potenciado por la presencia de más de una especie en el sistema (microcosmos. Con respecto a los niveles de Zn, dos de los organismos (L. hoffmeisteri

The efficacy of crude extract of Aloe secundiflora on Candida Albicans

African Journals Online (AJOL)

In- vitro studies on the efficacy of crude extracts of Aloe secundiflora on Candida albicans was conducted. Five mature leaves of Aloe secundiflora were collected and the crude extract was prepared, then autoclaved. The extract was then tested on Candida albicans grown on solid media. The results from these studies ...

The effect of aqueous Aloe vera gel extract on serum mineral ...

African Journals Online (AJOL)

This study was conducted to determine the effect of Aloe vera gel extract on the serum mineral compositions of Red Sokoto bucks. Blood samples were collected from 30 bucks before the commencement of administration of Aloe vera gel extract for serum minerals and these served as control group. The bucks were now ...

Relación existente entre la infección por los diferentes genotipos del Virus del Papiloma Humano y la presencia de patología premaligna y maligna del cuello uterino

OpenAIRE

Mazarico Gallego, Edurne

2012-01-01

1) Introducción Hasta el momento se han secuenciado total o parcialmente más de 100 tipos y subtipos de VPH. De todos ellos, aproximadamente 40 tipos se han aislado en lesiones de tracto genital inferior y unos 35, según diferentes estudios, en carcinomas. Según su riesgo oncogénico, se clasifican en tipos de VPH de bajo riesgo (VPH-BR), VPH de probable alto riesgo y VPH de alto riesgo (VPH-AR). Uno de los descubrimientos más importantes en la investigación etiológica del cáncer en los...

Optimality in Microwave-Assisted Drying of Aloe Vera ( Aloe barbadensis Miller) Gel using Response Surface Methodology and Artificial Neural Network Modeling

Science.gov (United States)

Das, Chandan; Das, Arijit; Kumar Golder, Animes

2016-10-01

The present work illustrates the Microwave-Assisted Drying (MWAD) characteristic of aloe vera gel combined with process optimization and artificial neural network modeling. The influence of microwave power (160-480 W), gel quantity (4-8 g) and drying time (1-9 min) on the moisture ratio was investigated. The drying of aloe gel exhibited typical diffusion-controlled characteristics with a predominant interaction between input power and drying time. Falling rate period was observed for the entire MWAD of aloe gel. Face-centered Central Composite Design (FCCD) developed a regression model to evaluate their effects on moisture ratio. The optimal MWAD conditions were established as microwave power of 227.9 W, sample amount of 4.47 g and 5.78 min drying time corresponding to the moisture ratio of 0.15. A computer-stimulated Artificial Neural Network (ANN) model was generated for mapping between process variables and the desired response. `Levenberg-Marquardt Back Propagation' algorithm with 3-5-1 architect gave the best prediction, and it showed a clear superiority over FCCD.

Evaluation of free radical scavenging and radioprotective efficacy of Aloe vera extract

International Nuclear Information System (INIS)

Chakrawarti, Aruna; Purohit, R.K.; Agarwal, Manisha; Ranga, Anita; Saran, Leena

2012-01-01

Radiation induced damage and lethality to the normal tissues can be partially reduced by the use of radio-protectors that lower down the damaging effects of radiation. In the present study radio-protective effect of Aloe vera extract which is rich in vitamins. It is basically cellular regenerator and therefore acts, with very positive results in many conditions. It is reported to have antioxidant, anti carcinogenic, antimicrobial and immunity stimulants properties. For the present experiment, healthy Swiss albino mice (6-8 weeks old) were selected and maintained under standard conditions of temperature and light. Aloe vera juice was fed orally at the dose rate of 0.01 m1/animal/day. The animals were divided into seven groups according to the treatment given to them i.e. given cadmium chloride solution as drinking water (group-II) or exposed to 5.0 Gy gamma radiations (group-III) or combined treatment of radiation and cadmium chloride (group-IV). The alterations in the biochemical parameters of all these group were compared with that of sham irradiated animals (group-I). The animals of experimental groups were given Aloe vera seven days prior to radiation or cadmium chloride treatment (group-V, VI and VII) respectively. All biochemical parameters of the control groups were compared with the respective experimental groups. An increase in the value of total proteins, glycogen, acid and alkaline phosphatase activity and RNA was observed up to day-14 in the non drug treated group and day-7 in the Aloe vera groups. Thereafter value declined up to day-28 without reaching to the normal. Whereas the value of cholesterol and DNA showed a decreasing trend up to day-14 in non drug treated groups and day-7 in Aloe vera treated groups. Thus it showed that cadmium and radiation produced toxic effect on kidney and Aloe vera minimize these effects. Protection offered by Aloe vera may be due to the scavenging or oxidizing free radicals. Thus it can be concluded that Aloe vera may

Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens.

Science.gov (United States)

Yim, Dongjean; Kang, Sang S; Kim, Dong W; Kim, Sang H; Lillehoj, Hyun S; Min, Wongi

2011-01-01

Aloes have been widely used for a broad range of pharmacological activities, including parasitic problems. Avian coccidiosis is the most costly and wide-spread parasitic disease in the poultry industry, and has been mainly controlled by the use of chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. In this study, the protective effects of Aloe vera-based diets were assessed in broiler chickens following oral infection with Eimeria maxima. Chickens were fed a regular diet supplemented with ground Aloe vera throughout the duration of the experiment beginning 2 days prior to infection with 1 × 10(4) sporulated oocysts of E. maxima. No significant differences were found in body weight gain or loss between the Aloe vera-supplemented and unsupplemented groups with or without E. maxima infections. Fecal oocyst shedding decreased significantly (p vera as compared to the unsupplemented group. Furthermore, the Aloe vera-supplemented group showed significantly fewer intestinal lesions (p vera could be used an alternative treatment for controlling avian coccidiosis. Copyright © 2010 Elsevier Inc. All rights reserved.

A comparison of the leaf gel extracts of Aloe ferox and Aloe vera in the topical treatment of atopic dermatitis in Balb/c mice.

Science.gov (United States)

Finberg, M J; Muntingh, G L; van Rensburg, C E J

2015-12-01

Aloe vera gel is widely used in the treatment of an array of disturbances, especially skin disorders. The wound-healing effects have been attributed to its moisturizing and anti-inflammatory effects as well as its beneficial effect on the maturation of collagen. The aim of the present study is to compare the effects of topically applied extracts of Aloe ferox with that of Aloe vera on the symptoms as well as IgE levels of a mouse model of atopic dermatitis (AD). Mice were sensitized and challenged with 2,4-dinitrochlorobenzene and treated afterwards for 10 consecutive days with the gels of either A. ferox or A. vera applied topically to the affected areas. A placebo gel was used for the control mice. Blood was collected at the beginning and end of the treatment period to measure serum IgE levels. Although the gels of both the Aloe species inhibited the cutaneous inflammatory response as well as serum IgE levels in the rats, the extracts of A. ferox were superior to that of A. vera in reducing IgE levels. The gels of A. ferox and A. vera, applied topically, may be a safe and useful alternative to antihistamines and topical corticosteroids, for the treatment of patients suffering from recurring chronic AD.

SINTESIS MEMBRAN NATA ALOE VERA-ETILENDIAMIN DAN KARAKTERISASINYA

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EB Susatyo

2014-06-01

Full Text Available Serat yang terkandung di dalam nata Aloe vera adalah selulosa sehingga dapat digunakan dalam sintesis membran. Telah dilakukan penelitian tentang sintesis membran nata Aloe vera-etilendiamin (nata-en menggunakan sistem vakum cair. Proses preparasi membran berlangsung melalui tiga tahap, yaitu preparasi nata Aloe vera, aktivasi menggunakan asam sulfat, dan modifikasi dengan menggunakan etilendiamin. Tujuan penelitian adalah untuk mempelajari teknik dalam sintesis membran nata-en kemudian melakukan karakterisasi untuk mengetahui karakter strukturnya. Hasil penelitian menunjukkan perilaku yang berbeda dalam hal sifat mekanik dan strukturnya. Membran nata murni memiliki sifat mekanik yang kuat, nata teraktivasi cenderung rapuh, sedangkan nata-en bersifat liat. Spektra infra merah dari ketiga tipe membran (nata murni, nata teraktivasi, dan nata-en secara umum tidak mengalami perubahan yang signifikan, hanya terjadi pergeseran panjang gelombang dari masing-masing membran. Berdasarkan spektra infra merah dapat diketahui bahwa masing-masing membran mempunyai gugus hidroksil, tetapi serapannya semakin melebar untuk setiap membran. Gugus alkil dan karboksil juga masih tampak, namun pada membran nata teraktivasi serapannya berkurang, sedangkan pada membran nata-en muncul puncak baru yang menunjukkan adanya gugus amin. Hal ini membuktikan bahwa telah terjadi ikatan antara nata dengan etilendiamin. Fibers contained in nata Aloe vera is cellulose that can be used in the synthesis of membrane. The research has done on the synthesis of nata Aloe vera-ethylenediamine (nata-en membrane by using liquid vacuum system. Membrane preparation process consisted of three stages, they are nata Aloe vera preparation, activation using sulfuric acid, and membrane modification by using ethylenediamine. The purpose of research is to study the technique of nata-en membrane synthesis then to perform the characterization to determine the character of their structure.The results

In vivo safety evaluation of UP780, a standardized composition of aloe chromone aloesin formulated with an Aloe vera inner leaf fillet.

Science.gov (United States)

Yimam, Mesfin; Brownell, Lidia; Jia, Qi

2014-08-01

Safety profiles of the aloe chromone aloesin or Aloe vera inner leaf fillet (Qmatrix) as a well tolerated entity have been reported separately. UP780, a standardized composition of aloe chromone formulated with an Aloe vera inner leaf fillet, has shown a significant beneficial effect in lowering blood glucose and improving insulin resistance in human. Here we evaluate the safety of UP780 after a repeated 14 and 90-day oral administration in CD-1 mice. UP780 was given at doses of 100mg/kg/day, 500mg/kg/day and 1000mg/kg/day to groups of 10 male and 10 female for 90days or administered by oral gavage at a dose of 2g/kg/day to groups of 5 male and 5 female for 14days. Body weight, feed consumption, hematology, clinical chemistry and histopathologic evaluation were performed. UP780 at a dose of 1000mg/kg/day or at 2000mg/kg/day produced no treatment-related toxicity or mortality. Body weight gain or feed consumption was similar between groups. There was no test article-related microscopic change. Spontaneously occurring minor changes in clinical chemistry and hematology were observed. However, these changes were limited to one sex or were not dose correlated. UP780 was well tolerated in this strain. A dose of 2000mg/kg/day was identified as the NOAEL (no-observed-adverse-effect-level). Copyright © 2014 Elsevier Inc. All rights reserved.

Modulation of radiation injuries in rats receiving multiple doses of Aloe Vera

International Nuclear Information System (INIS)

Azab, Kh.SH.

2007-01-01

This study has been performed to examine the efficacy of Aloe vera juice (Aloe barbadensis Miller) against radiation injuries of 7 Gy whole body gamma irradiation (single dose). Inductions of lipid peroxidation (thiobarbituric acid reactive substances, (TBARS)) of biomembranes lipids as well as the subsequent changes in the activities of subcellular organelle marker enzymes were discussed. Activities of glutamate dehydrogenase (GDH), acid phosphatase and glucose-6-phosphatase (marker enzymes of mitochondria, lysosome and microsomes, respectively) were estimated. The activities of these enzymes were also measured in cytosol. Subcellular fractionation's were performed in liver, heart and spleen tissues. Aloe vera was supplemented daily to rats (0.25 ml/kg body wt/day) by gavage, 5 days before irradiation and treatment was extended for 10 days post irradiation. Experimental investigations were performed on the 3 rd and 10 th day after exposure to radiation. The results obtained indicated that, Aloe vera administration has significantly minimized the radiation-induced increase in the amount of TBARS in different cell fractions as compared with control rats. Significant amelioration in the activities of organelles marker enzymes GDH, acid phosphatase and glucose-6-phosphatase was observed from 3 rd up to 10 th days for the 3 tissues. The results also detected improvement in cytosolic enzyme activities due to Aloe vera intake. It could be suggested that the diverse active constituents of Aloe vera play a significant role in decreasing the peroxidation of subcellular membrane lipids induced by radiation exposure, prevent diffusion of organelle enzymes to cytosol and consequently salvage the integrity of living cell

Modulatory Role of Aloe vera on Gamma Irradiation Induced Histological Changes in Different Tissues of Rats

International Nuclear Information System (INIS)

Rezk, R.G.

2005-01-01

Aloe Vera is known for its wide medicinal properties. This study was performed to evaluate the role of Aloe vera (Aloe barbadensis Miller) in the amelioration of the histological disorders that occurr in different tissues of albino rats exposed to 7 Gy whole body gamma irradiation, delivered as a single dose. Aloe vera (leaf juice filtrate) was supplemented daily to rats (0.25 ml/kg b wt/day) by gavage, 5 days before irradiation and 10 days after irradiation. Experimental investigations performed 7 and 10 days after exposure to radiation showed that Aloe vera treatment has significantly improved the radiation-induced inflammation, haemorrhage, widening and dilated blood vessela, necrosis, atrophy sloughing in liver, spleen and small intestine (jejenum) tissues of irradiated rats. It is concluded that the synergistic relationship between the elements found in the leaf of Aloe vera could be a useful adjunct for maintaining the integrity of histological architecture

effect of aloe barbadensis on the lipid profile and fasting blood sugar ...

African Journals Online (AJOL)

Our results bring to the fore again, the link between hyperlipidemia (coronary heart disease) and increased sugar concentration in the body (diabetes) and show that Aloe barbadensis has protective effects against these two pathological states. KEY WORDS: Aloe barbadensis; hypercholesterolemia; fasting blood sugar; ...

Aloe vera herbal dentifrices for plaque and gingivitis control: a systematic review.

Science.gov (United States)

Dhingra, K

2014-04-01

To evaluate the effectiveness of aloe vera containing herbal dentifrices in improving plaque control and gingival health. A manual and electronic literature (MEDLINE and Cochrane Central Register of Controlled Trials) search was performed up to July 2012, for randomized controlled trials presenting clinical, microbiological, immunological, and patient-centered data for the efficacy of aloe vera herbal dentifrices for controlling plaque and gingival inflammation in patients with gingivitis. From 79 titles and abstracts, eight full-text articles were screened and finally two randomized controlled trials were selected. These randomized controlled trials reported that aloe vera dentifrices were similar in efficacy to control dentifrices in effectively reducing plaque and gingival inflammation in gingivitis patients based on the assessment of clinical, microbiological, and patient-centered treatment outcomes. However, many important details (composition and characteristics of aloe vera and control dentifrices along with appropriate randomization, blinding, and outcomes assessed) were lacking in these trials, and therefore, the quality of reporting and methods was generally flawed with high risk of bias. Even though there are some promising results, the clinical effectiveness of aloe vera herbal dentifrices is not sufficiently defined at present and warrants further investigations based on reporting guidelines of herbal CONSORT statement. © 2013 John Wiley & Sons A/S.

A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study).

Science.gov (United States)

Ehsani, Maryam; Amin Marashi, Mahmood; Zabihi, Ebrahim; Issazadeh, Maryam; Khafri, Soraya

2013-01-01

Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk diffusion, microdilution and direct contact test. In addition to the above bacterium, the Aloe vera gel effect on Staphylococcus aureus and Streptococcus mutans was evaluated. Disk diffusion test revealed that propolis ethanolic extracts (the alcohol concentration of 15 and 40%) and Aloe vera gel have antibacterial activities but aqueous extract of propolis did not show any effect in this test. The MICs for propolis ethanolic extracts, Aloe vera gel and aqueous extract of propolis (0% alcohol) were 313 µg/ml, 750 µg/ml, 2250 µg/ml, and ≥ 500 µg/ml respectively, much higher than the Chlorhexidine one. In direct contact test, contrary to Aloe vera, all three propolis extracts showed antibacterial effects on E. faecalis. The Aloe vera gel also showed significant antibacterial effect on S.aureus and S.mutans. The hydroalcoholic extracts of propolis and Aloe vera gel had antibacterial effects on E. faecalis, however, propolis is more potent than Aloe vera. The antibacterial effect of Aloe vera on S. aureus and S. mutans is low (MIC ≥ 2250 µg/ml). Appropriate concentrations of alcoholic extracts of propolis and some fractions of Aloe vera gel might be good choices for disinfecting the root canal in endodontic treatments.

Anticonvulsant activity of Aloe vera leaf extract in acute and chronic models of epilepsy in mice.

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Rathor, Naveen; Arora, Tarun; Manocha, Sachin; Patil, Amol N; Mediratta, Pramod K; Sharma, Krishna K

2014-03-01

The effect of Aloe vera in epilepsy has not yet been explored. This study was done to explore the effect of aqueous extract of Aloe vera leaf powder on three acute and one chronic model of epilepsy. In acute study, aqueous extract of Aloe vera leaf (extract) powder was administered in doses 100, 200 and 400 mg/kg p.o. Dose of 400 mg/kg of Aloe vera leaf extract was chosen for chronic administration. Oxidative stress parameters viz. malondialdehyde (MDA) and reduced glutathione (GSH) were also estimated in brain of kindled animals. In acute study, Aloe vera leaf (extract) powder in a dose-dependent manner significantly decreased duration of tonic hind limb extension in maximal electroshock seizure model, increased seizure threshold current in increasing current electroshock seizure model, and increased latency to onset and decreased duration of clonic convulsion in pentylenetetrazole (PTZ) model as compared with control group. In chronic study, Aloe vera leaf (extract) powder prevented progression of kindling in PTZ-kindled mice. Aloe vera leaf (extract) powder 400 mg/kg p.o. also reduced brain levels of MDA and increased GSH levels as compared to the PTZ-kindled non-treated group. The results of study showed that Aloe vera leaf (extract) powder possessed significant anticonvulsant and anti-oxidant activity. © 2013 Royal Pharmaceutical Society.

Accuracy assessment of ALOS optical instruments: PRISM and AVNIR-2

Science.gov (United States)

Tadono, Takeo; Shimada, Masanobu; Iwata, Takanori; Takaku, Junichi; Kawamoto, Sachi

2017-11-01

This paper describes the updated results of calibration and validation to assess the accuracies for optical instruments onboard the Advanced Land Observing Satellite (ALOS, nicknamed "Daichi"), which was successfully launched on January 24th, 2006 and it is continuously operating very well. ALOS has an L-band Synthetic Aperture Radar called PALSAR and two optical instruments i.e. the Panchromatic Remotesensing Instrument for Stereo Mapping (PRISM) and the Advanced Visible and Near Infrared Radiometer type-2 (AVNIR-2). PRISM consists of three radiometers and is used to derive a digital surface model (DSM) with high spatial resolution that is an objective of the ALOS mission. Therefore, geometric calibration is important in generating a precise DSM with stereo pair images of PRISM. AVNIR-2 has four radiometric bands from blue to near infrared and uses for regional environment and disaster monitoring etc. The radiometric calibration and image quality evaluation are also important for AVNIR-2 as well as PRISM. This paper describes updated results of geometric calibration including geolocation determination accuracy evaluations of PRISM and AVNIR-2, image quality evaluation of PRISM, and validation of generated PRISM DSM. These works will be done during the ALOS mission life as an operational calibration to keep absolute accuracies of the standard products.

Karyological studies in ten different populations of desert lily aloe vera from pakistan

International Nuclear Information System (INIS)

Jahan, B.; Vahidy, A.A.

2014-01-01

To enhance theoretical basis of Aloe feeding and provide cytological basement, the karyotype and morphology of mitotic chromosomes, ten different populations of Aloe vera collected from various geographical locations of Karachi, Pakistan were studied by aceto-orcein staining technique. The results showed that chromosome number of Aloe vera is 2n=14. The karyotype is bimodal and consists of 14 chromosomes (8 large and 6 small) predominantly with submedian, median and subterminal centromere. Average chromosome lengths among populations ranged from 7.95-2.36 micro m. (author)

Molecular interaction and energy transfer between human serum albumin and bioactive component Aloe dihydrocoumarin

Science.gov (United States)

Zhang, Xiu-Feng; Xie, Ling; Liu, Yang; Xiang, Jun-Feng; Li, Lin; Tang, Ya-Lin

2008-10-01

Aloe dihydrocoumarin is an antioxidant and a candidate of immunomodulatory drug on the immune system and can balance physiological reactive oxygen species (ROS) levels which may be useful to maintain homeostasis. In order to explore the mechanism of drug action at a molecular level, the binding of Aloe dihydrocoumarin with human serum albumin (HSA) has been investigated by fluorescence, ultraviolet (UV), circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy, fluorescence dynamics, and molecular dynamic docking for the first time. We observed a quenching of fluorescence of HSA in the presence of Aloe dihydrocoumarin and also analyzed the quenching results using the Stern-Volmer equation and obtained high affinity binding to HSA. A Förster type fluorescence resonance energy transfer mechanism is involved in this quenching of Trp fluorescence by Aloe dihydrocoumarin. From the CD and FT-IR results, it is apparent that the interaction of Aloe dihydrocoumarin with HSA causes a conformational change of the protein, with the loss of α-helix stability and the gain of β-sheet and β-turn content. Data obtained by fluorescence spectroscopy, fluorescence dynamics, CD, and FT-IR experiments along with the docking studies suggest that Aloe dihydrocoumarin binds to residues located in subdomain IIA of HSA.

Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation.

Science.gov (United States)

Carvalho, Nayane Chagas; Guedes, Simone Alves Garcez; Albuquerque-Júnior, Ricardo Luiz Cavalcanti; de Albuquerque, Diana Santana; de Souza Araújo, Adriano Antunes; Paranhos, Luiz Renato; Camargo, Samira Esteves Afonso; Ribeiro, Maria Amália Gonzaga

2018-01-01

This study aims to evaluate, in vitro, the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled water; AL - Aloe vera with distilled water and FTL; HA - calcium hydroxide P.A. with distilled water; HL - calcium hydroxide P.A. with distilled water and FTL; HAA - calcium hydroxide P.A. with Aloe vera and distilled water; HAL - calcium hydroxide P.A. with Aloe vera, distilled water, and FTL. The cytotoxicity was evaluated by MTT assay at 24, 48, and 72h and the genotoxicity by micronucleus test assay. This study was performed in triplicate. Data obtained in both tests were statistically analyzed by ANOVA and Tukey's tests (p≤0.05). Group AA presented high genotoxicity and low cytotoxicity. After 24, 48, and 72h, the group HAA significantly reduced the cell viability. Interaction with FTL showed slightly increase cell viability after 24 and 48h in groups CL and HL (pAloe vera allowed higher cell viability in human pulp fibroblasts in the presence of calcium hydroxide or with FTL separately, but genotoxicity increased in these associations. Copyright © 2017 Elsevier B.V. All rights reserved.

Dietary Aloe vera components' effects on cholesterol lowering and estrogenic responses in juvenile goldfish, Carassius auratus.

Science.gov (United States)

Palermo, Francesco A; Cocci, Paolo; Angeletti, Mauro; Felici, Alberto; Polzonetti-Magni, Alberta Maria; Mosconi, Gilberto

2013-08-01

Aloes are now considered a very interesting source of bioactive compounds among which phytosterols should play a major role. The present study is an attempt to investigate the hypocholesterolemic activity of Aloe vera associated with its impact on the reproductive status of juvenile goldfish. Therefore, the short- and long-term effects of feeding supplementary diet containing aloe components (20 mg aloe/g diet; 2%) on plasma lipids, plasma vitellogenin, and hepatic estrogen receptor α/β1 mRNA levels in goldfish were examined. Results of GC-MS for phytosterols show high abundance of β-sitosterol in freeze-dried powder of Aloe vera whole leaves. Moreover, a 2% aloe powder dietary supplement was not found estrogenic in juvenile goldfish after either 7- or 30-day treatment, but was consistent in plasma hypocholesterolemic effects following long-term exposure. The present data further support that plasma cholesterol modulation induced by phytosterols may not be related to estrogen-like activity.

Antibacterial Activity of Anthraquinone from Aloe on Spiced Pig Head

Science.gov (United States)

Xu, Lingyi; Li, Xiao; Cui, Yuqian; Pang, Meixia; Wang, Fang; Qi, Jinghua

2017-12-01

[Objective] To optimize the extraction of anthraquinone from Aloe by ultrasonic extraction and its antibacterialactivity. [Method]The influences of different extraction time and ethanol concentration, on anthraquinone contentwere evaluated by asingle factor experiment. And anthraquinone content was determined by ultraviolet spectrophotometry. The bacteriostasis of anthraquinone on spiced pig head’s common putrefying bacteria: Staphylococcus, Serratieae, Bacillus, Proteus and the minimal inhibitory concentration (MIC) were studied by oxford plate assay system. [Result]The best extraction time was 30 minutes and the best ethanol concentration was 80%. The antibacterial activity of the Aloe anthraquinone on Staphylococcus Aureus, Bacillus Proteus is obviously, the minimum inhibitory concentrations were 0.0625 g/mL, 0.05 g/mL, 0.125 g/mL respectively and no inhibitory effect on Serratieae. [Conclusions] The anthraquinones from Aloe can inhibit a part Of spoilage bacteria inspiced pig heads.

Efectividad de la crema de Aloe vera en pacientes con psoriasis vulgar de la Parroquia San Fernando

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Yoisel Belen Ruiz

2014-10-01

Full Text Available Se realizó un estudio de intervención terapéutica en 20 pacientes con psoriasis vulgar, pertenecientes a la Parroquia San Fernando, en Montes, Estado Sucre (Venezuela, de mayo a diciembre del 2013, quienes fueron seleccionados aleatoriamente y asignados en 2 grupos de idéntica cantidad de integrantes: el de control, que utilizó medicación convencional con triamcinolona, y el de estudio, que recibió tratamiento tópico con crema de Aloe vera (sábila de base inerte; a fin de determinar la efectividad de esta última. Para establecer la comparación entre ambos grupos, se emplearon técnicas no paramétricas debido al número reducido de pacientes, y siempre se consideró un nivel de significación de 0,05. Se obtuvo que la modalidad terapéutica con crema de sábila fuera tan efectiva como la aplicación de la triamcinolona, pero a diferencia de esta última, no presentó reacciones adversas. Así, pudo concluirse que la terapia con Aloe vera es efectiva para tratar pacientes con psoriasis vulgar

Holistic Healing Through Herbs: Effectiveness of Aloe Vera on Post Extraction Socket Healing.

Science.gov (United States)

Nimma, Vijaya Lakshmi; Talla, Harsha Vardhan; Bairi, Jaya Krishna; Gopaldas, Madhulatha; Bathula, Haritha; Vangdoth, Sandeep

2017-03-01

Advances in the field of alternative medicine has encouraged the use of various natural products for multiple uses in the field of dentistry for treatment of various oral diseases. A natural herbal product is Aloe vera, which has number of benefits with no reported side effects and gaining considerable importance in clinical research. The aim of this cross-sectional randomized interventional study was to evaluate the effectiveness of Aloe vera in the healing of post extraction sockets. A sample size of 40 patients undergoing atraumatic extraction was divided into two groups. Group A-patients were prescribed only analgesics and followed for seven consecutive days and socket healing assessment was done. Group B-Patients were given Aloe vera soaked gel foams, followed up to the third and seventh day by two observers and the socket healing was assessed using the standardized index by Landry, Turnbull and Howley. In addition to healing the common complaint associated with extraction wound, the pain was assessed using numerical rating scale. Data obtained was statistically analysed using Mann-whitney U test, Wilcoxon Signed ranks test and Spearman's rank correlation method. Control group on the third and the seventh day showed healing of 60% and 70% respectively and the Aloe vera group showed a better result having a healing potential of 70% on the third day and 90% on the seventh day which was statistically significant with a (p-valueAloe vera group showed a significant decrease in pain after two hours on the day of extraction followed by second, third and seventh day which was statistically significant (p-valueAloe vera has been proved to have a unique property that is implicated in better healing than other group without any side effects. Aloe vera is economical, effective, powerful nutritional supplement and antioxidant that protects and promotes wound healing.

Holistic Healing Through Herbs: Effectiveness of Aloe Vera on Post Extraction Socket Healing

Science.gov (United States)

Talla, Harsha Vardhan; Bairi, Jaya Krishna; Gopaldas, Madhulatha; Bathula, Haritha; Vangdoth, Sandeep

2017-01-01

Introduction Advances in the field of alternative medicine has encouraged the use of various natural products for multiple uses in the field of dentistry for treatment of various oral diseases. A natural herbal product is Aloe vera, which has number of benefits with no reported side effects and gaining considerable importance in clinical research. Aim The aim of this cross-sectional randomized interventional study was to evaluate the effectiveness of Aloe vera in the healing of post extraction sockets. Materials and Methods A sample size of 40 patients undergoing atraumatic extraction was divided into two groups. Group A-patients were prescribed only analgesics and followed for seven consecutive days and socket healing assessment was done. Group B-Patients were given Aloe vera soaked gel foams, followed up to the third and seventh day by two observers and the socket healing was assessed using the standardized index by Landry, Turnbull and Howley. In addition to healing the common complaint associated with extraction wound, the pain was assessed using numerical rating scale. Data obtained was statistically analysed using Mann-whitney U test, Wilcoxon Signed ranks test and Spearman’s rank correlation method. Results Control group on the third and the seventh day showed healing of 60% and 70% respectively and the Aloe vera group showed a better result having a healing potential of 70% on the third day and 90% on the seventh day which was statistically significant with a (p-valueAloe vera group showed a significant decrease in pain after two hours on the day of extraction followed by second, third and seventh day which was statistically significant (p-valueAloe vera has been proved to have a unique property that is implicated in better healing than other group without any side effects. Aloe vera is economical, effective, powerful nutritional supplement and antioxidant that protects and promotes wound healing. PMID:28511517

The effect of aloe vera bioactive level as feed additive on the egg performances of laying hens

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I.A.K Bintang

2005-06-01

Full Text Available A study on the use of aloe vera bioactives as feed additive in layer ration was conducted. One hundred and twenty pullets strain Isa Brown were allocated into 5 treatments with 6 replicates and 4 birds/replicate. The treatments were: control,control+antibiotic (50 ppm zinc bacitracin, and control+ aloe vera at three levels (0.25; 0.50 and 1.00 g/kg. The treatments were conducted in a completely randomized design. Parameter measured were first initial body weight, age at 1st lay, feed intake, egg weight, hen day (%HD and feed conversion ratio. The results showed that antibiotic and aloe vera used as additive for 9 months production did not significantly (P<0.05 affect all parameter measured, except feed intake of hens fed diet containing 0.5 g/kg aloe vera was significantly (P<0.05 higher than control. The addition of aloe vera at 1.0 g/kg significantly (P<0.05 reduced the feed intake as compared with the control, aloe vera 0.25 and 0.50 g/kg. The used of aloe vera (1.00 g/kg produced egg weight significantly (P<0.05 higher than the control, and feed conversion ratio was significantly (P<0.05 better than the control and aloe vera (0.25 g/kg. It is concluded that the best treatment was the diet with aloe vera level at 1.00 g/kg. This treatment improved feed efficiency 8.40%.

Aloe vera: an in vitro study of effects on corneal wound closure and collagenase activity.

Science.gov (United States)

Curto, Elizabeth M; Labelle, Amber; Chandler, Heather L

2014-11-01

To evaluate the in vitro effects of an aloe vera solution on (i) the viability and wound healing response of corneal cells and (ii) the ability to alter collagenase and gelatinase activities. Primary cultures of corneal epithelial cells and fibroblasts were prepared from grossly normal enucleated canine globes and treated with an aloe solution (doses ranging from 0.0-2 mg/mL). Cellular viability was evaluated using a colorimetric assay. A corneal wound healing model was used to quantify cellular ingrowth across a defect made on the confluent surface. Anticollagenase and antigelatinase activities were evaluated by incubating a bacterial collagenase/gelatinase with aloe solution (doses ranging from 0.0-500 μg/mL) and comparing outcome measures to a general metalloproteinase inhibitor, 1, 10-phenanthroline, and canine serum (doses ranging from 0.0-100%). None of the concentrations of aloe solution tested significantly affected the viability of corneal epithelial cells or fibroblasts. Concentrations ≤175 μg/mL slightly accelerated corneal epithelial cell wound closure; this change was not significant. Concentrations ≥175 μg/mL significantly (P ≤ 0.001) slowed the rate of corneal fibroblast wound closure, while aloe concentrations Aloe solution did not alter the ability for collagenase to degrade gelatin or collagen Type I but increased the ability for collagenase to degrade Type IV collagen. Although additional experiments are required, lower concentrations of aloe solution may be beneficial in healing of superficial corneal wounds to help decrease fibrosis and speed epithelialization. An increase in collagenase activity with aloe vera warrants further testing before considering in vivo studies. © 2014 American College of Veterinary Ophthalmologists.

Menurunkan Derajat Flebitis Akibat Terapi Intravena pada Anak dengan Kompres Aloe Vera: Studi Pilot

OpenAIRE

Hasanah, Oswati; Novayelinda, Riri; Maifera, Maifera; Isdelni, Isdelni

2017-01-01

Reducing the Grade of Phlebitis due to Intravenous Therapy in Children with Aloe Vera Compress. Intravenous therapy is a type of treatment that is widely provided to hospitalized children. Intravenous application in a long time causes complications in children, such as phlebitis. One of the interventions in handling phlebitis is compressing Aloe vera which is useful because it has electrolyte in low concentration so as not to cause extravasation. This study aims to identify the effect of Aloe...

Meristem Culture of Aloe vera L

International Nuclear Information System (INIS)

Kay Thi Mya; Thida Myint; Myo Han; Khin Maung Sein

2005-10-01

Shoot tips of Aloe vera L were excised and inoculated in basal and modified MS (Murashige and Skoog, 1962) medium. Growth regulators and growth factors were supplemented to the basal medium. Development of explants, initiation of callus and multiplication of shoots in culture were recorded.

The effects of Aloe vera [gel] on clotting time, prothrombin time and ...

African Journals Online (AJOL)

Background: Published reports on the effects of Aloe vera gel on blood coagulation in experimental animals are relatively scanty. Aim: To determine the effect of Aloe vera gel on clotting time, prothrombin time and plasma fibrinogen concentration in albino Wistar rats. Methods: A total of 28 adult albino Wistar rats divided ...

Diffusion of hydroxyl ions from calcium hydroxide and Aloe vera pastes.

Science.gov (United States)

Batista, Victor Eduardo de Souza; Olian, Douglas Dáquila; Mori, Graziela Garrido

2014-01-01

This study evaluated the diffusion through the dentinal tubules of hydroxyl ions from different calcium hydroxide (CH) pastes containing Aloe vera. Sixty single-rooted bovine teeth were used. The tooth crowns were removed, the root canals were instrumented and the specimens were assigned to 4 groups (n=15) according to the intracanal medication: Group CH/S - CH powder and saline paste; Group CH/P - CH powder and propylene glycol paste; Group CH/A - calcium hydroxide powder and Aloe vera gel paste; Group CH/A/P - CH powder, Aloe vera powder and propylene glycol paste. After placement of the root canal dressings, the teeth were sealed coronally and apically with a two-step epoxy adhesive. The teeth were placed in identified flasks containing deionized water and stored in an oven with 100% humidity at 37 °C. After 3 h, 24 h, 72 h, 7 days, 15 days and 30 days, the deionized water in the flasks was collected and its pH was measured by a pH meter. The obtained data were subjected to statistical analysis at a significance level of 5%. The results demonstrated that all pastes provided diffusion of hydroxyl ions through the dentinal tubules. The combination of Aloe vera and CH (group CH/A) provided a constant release of calcium ions. Group CH/A/P showed the highest pH at 24 and 72 h. In conclusion, the experimental pastes containing Aloe vera were able to enable the diffusion of hydroxyl ions through the dentinal tubules.

Reproductive potential of male catfish treated with gel extract of Aloe ...

African Journals Online (AJOL)

The reproductive potential of male catfish treated with gel extract of Aloe vera plant was studied using twelve male fish weighing 500-560g.The fish were divided into 3 groups; A, B and C with four fish in each group. Group A was treated with 2% Aloe vera gel while group B was treated with 3% and Group C the control was ...

Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par. Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L.

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Betty Stefany Lozada

2012-04-01

Full Text Available Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs (AE1, AE2, AE3, AE4 y AE5 extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5 fueron caracterizados por su morfología, germinación y crecimiento en medios de cultivo. La actividad antifúngica de diferentes concentraciones de los AEs fue evaluada contra el aislado M2 y la cepa de M. roreri (ATCC 64239, para determinar su efecto sobre la germinación y la inhibición del crecimiento micelial. Los AEs estudiados inhibieron 100% de la germinación y del crecimiento micelial cuando fueron utilizados en concentraciones de 800 - 1000 µg/ml. Concentraciones de 200 µg/ml también mostraron efecto sobre los aislamientos fúngicos, siendo los AEs obtenidos de L. origanoides (AE2 y AE3 los más activos. Estos estaban compuestos principalmente por timol, p-cimeno, g-terpineno, acetato de timilo, carvacrol, b-mirceno, trans-b-cariofileno. Diferencias significativas (P < 0.05 sobre la susceptibilidad se observaron entre las dos cepas fúngicas estudiadas, siendo en general más susceptible el aislado M2 que la cepa ATCC. Los AEs de L. origanoides son candidatos para ser usados como posibles biofungicidas en el control de la moniliasis. Son necesarios estudios futuros orientados a determinar la actividad in vivo antifúngica de estos AEs y sus principales componentes.

Aloe vera gel provides protection to Swiss albino mice against radiation induced injury

International Nuclear Information System (INIS)

Saini, D.K.; Chaudhary, G.; Saini, M.R.

2007-01-01

Aloe gel powder was administered orally to Swiss albino mice at the dose of 250, 500 and 750 mg/kg body weight/day for 15 consecutive days with and without irradiation with 8 Gy to find out its maximum effective radioprotective dose. 30 days survivality and change in levels of lipid peroxidation (LPO), reduced glutathione (GSH) and vitamin C were studied at 1 hour and 30 days for this purpose. In Aloe treated (750 mg/kg b. wt.) 8 Gy irradiated animals 30 days survivality, contents of GSH and vitamin C were higher and LPO level was lesser at 1 hour and 30 days in comparison to those treated with Aloe at the dose of 250 and 500 mg/kg body weight and irradiated with 8 Gy. These results indicate that Aloe administration at the dose of 750 mg/kg body weight was found to be the most effective

SYNTHESIS AND CHARACTERIZATION OF BIODEGRDABLE PLASTIC FROM CASAVA STARCH AND ALOE VERA EXTRACT WITH GLYCEROL PLASTICIZER

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Mery Apriyani

2016-05-01

Full Text Available Synthesis and characterizations of Biodegradable Plastic made of Cassava Waste Starch, glycerol, acetic acid and Aloe vera extract has done. The aims of this research are to study the influence of addition of aloe vera extract in plastics mechanics properties, water vapor transmission rate and biodegradation. There are five main steps in this research, extraction of aloe vera, cassava starch preparation from cassava waste, preparations, characterization and biodegradability study of biodegradable plastic. The addition variations of aloe vera extract that used in this research are 0.01; 0.03; 0.05; 0.07 and 0.14 grams. Results showed that the addition of aloe Vera tends to increased biodegrable plastic thickness to 0.01 mm and elongation to 32.07%. However, biodegradable plastic tensile strength tends to decreased to 23.95 Mpa. Optimum tensile strength is 3.90 Mpa and elongation is 34.43%. Optimum water vapor transmission rate is 2.40 g/m2hours. Biodegradation study of biodegradable plastic showed that addition of aloe vera extract doesn’t significantly influence in plastic degradations.

Comparison of aloe vera and omeprazole in the treatment of equine gastric ulcer syndrome.

Science.gov (United States)

Bush, J; van den Boom, R; Franklin, S

2018-01-01

Anecdotally, aloe vera is used to treat gastric ulceration, although no studies have yet investigated its efficacy in horses. To test the hypothesis that aloe vera would be noninferior to omeprazole in the treatment of equine gastric ulcer syndrome. Randomised, blinded clinical trial. Forty horses with grade ≥2 lesions of the squamous and/or glandular mucosa were randomly assigned to one of two groups. Horses received either aloe vera inner leaf gel (17.6 mg/kg bwt) b.i.d. or omeprazole (4 mg/kg bwt) s.i.d. for approximately 28 days, after which a repeat gastroscopic examination was performed to determine disease resolution. Horses with persistent lesions were offered a further 28 days of treatment with omeprazole (4 mg/kg bwt s.i.d.) and were re-examined on completion of treatment. Efficacy analyses were based on 39 horses that completed the trial. Equine squamous gastric disease (ESGD) was observed in 38 horses; improvement and healing rates in these horses were 56% and 17%, respectively, in the aloe vera group, and 85% and 75%, respectively, in the omeprazole group. Healing was less likely to occur in horses with prolonged gastric emptying. Equine glandular gastric disease (EGGD) was less common than ESGD (n = 14) and numbers were too small to perform meaningful statistical analyses. The hypothesis that aloe vera would be noninferior to omeprazole was not supported. No placebo control group was included. Limited numbers preclude any comment on the efficacy of aloe vera in the treatment of EGGD. Treatment with aloe vera was inferior to treatment with omeprazole. © 2017 EVJ Ltd.

The effect of Aloe vera gel on viability of dental pulp stem cells.

Science.gov (United States)

Sholehvar, Fatemeh; Mehrabani, Davood; Yaghmaei, Parichehr; Vahdati, Akbar

2016-10-01

Dental pulp stem cells (DPSCs) can play a prominent role in tissue regeneration. Aloe vera L. (Liliaceae) contains the polysaccharide of acemannan that was shown to be a trigger factor for cell proliferation, differentiation, mineralization, and dentin formation. This study sought to determine the viability of DPSCs in Aloe vera in comparison with Hank's balanced salt solution (HBSS). Twelve rabbits underwent anesthesia, and their incisor teeth were extracted; the pulp tissue was removed, chopped, treated with collagenase and plated in culture flasks. DPSCs from passage 3 were cultured in 24-well plates, and after 3 days, the culture media changed to 10, 25, 50, and 100% concentrations of Aloe vera at intervals of 45 and 90 min and 3 and 6 h. Distilled water was used as negative and HBSS as positive control for comparison. The cell morphology, viability, population doubling time (PDT), and growth kinetics were evaluated. RT-PCR was carried out for characterization and karyotyping for chromosomal stability. Aloe vera showed a significant higher viability than HBSS (74.74%). The 50% Aloe vera showed higher viability (97.73%) than other concentrations. PDT in 50% concentration was 35.1 h and for HBSS was 49.5 h. DPSCs were spindle shaped and were positive for CD73 and negative for CD34 and CD45. Karyotyping was normal. Aloe vera as an inexpensive and available herb can improve survival of avulsed or broken teeth in emergency cases as a transfer media. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Uso de cobertura com colágeno e aloe vera no tratamento de ferida isquêmica: estudo de caso El uso de colágeno y aloe vera en el tratamiento de la herida isquémica: estudio de caso Use of collagen and aloe vera in ischemic wound treatment: study case

Directory of Open Access Journals (Sweden)

Simone Helena dos Santos Oliveira

2010-06-01

Full Text Available O presente estudo relata o caso clínico de um paciente diabético e hipertenso, portador de ferida isquêmica, tratado com cobertura não convencional, à base de Aloe vera e colágeno. Faz parte de um projeto de pesquisa experimental, coordenado por professores e enfermeiros que atuam em projetos para o desenvolvimento de novas tecnologias para o tratamento de feridas. O caso em tela foi selecionado dentre os dos demais pacientes acompanhados. A coleta de dados foi efetuada através da anamnese e exame físico do paciente, utilizando-se um instrumento com dados relativos às condições do paciente e da lesão, bem como através do registro fotográfico da lesão. Os curativos foram realizados diariamente e, ao final de aproximadamente dez semanas, observou-se a total cicatrização da lesão. Não foram observados desconfortos ou complicações decorrentes do uso do produto, concluindo-se que o mesmo apresentou boa tolerabilidade e eficácia terapêutica para este caso em particular.Este estudio reporta el caso clínico de un paciente hipertenso, diabético, con una herida isquémica tratada con apósitos no convencionales de Aloe vera y colágeno, y forma parte de un proyecto de investigación experimental coordinado por profesores y enfermeros que trabajan en proyectos de desarrollo de nuevas tecnologías para el tratamiento de heridas. El caso de referencia fue elegido entre muchos pacientes en seguimiento. Los datos fueron obtenidos por la anamnesis y el examen físico del paciente, utilizándose un instrumento con datos relativos a las condiciones del paciente y de la lesión, así como un registro fotográfico de la lesión. Las curaciones se realizaron diariamente, al cabo de aproximadamente diez semanas se obtuvo la curación total. No se observó incomodidad o cualquier otra complicación derivada de la utilización del producto, por lo que se concluye en que el tratamiento demostró una buena tolerabilidad y eficacia terap

Bacterial leaf rot of Aloe vera L., caused byErwinia chrysanthemi biovar 3

NARCIS (Netherlands)

Laat, de P.C.A.; Verhoeven, J.T.W.; Danse, J.D.

1994-01-01

A severe attack of the bacteriumErwinia chrysantemi biovar 3 on the succulentAloe vera on the Carribean island of Aruba is described. Biochemical and pathological characteristics of strains are presented, including results of successful inoculation experiments onAloe vera. This is the first report

CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

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Juan Carlos Castro Gómez

2012-12-01

Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

Review of Clinical Pharmacology of Aloe vera L. in the Treatment of Psoriasis.

Science.gov (United States)

Miroddi, Marco; Navarra, Michele; Calapai, Fabrizio; Mancari, Ferdinando; Giofrè, Salvatore Vincenzo; Gangemi, Sebastiano; Calapai, Gioacchino

2015-05-01

Aloe vera L., is a plant used worldwide as folk remedy for the treatment of various ailments, including skin disorders. Its gel is present in cosmetics, medicinal products and food supplements. Psoriasis, an immune-mediated chronic inflammatory disease, involving mainly the skin, affects about the 2-3% of general population. Conventional pharmacological treatments for psoriasis can have limited effectiveness and can cause adverse reactions. For this reason often psoriatic patients look for alternative treatments based on natural products containing Aloe vera. We conducted a systematic review of clinical trials assessing effectiveness and safety of aloe for the treatment of psoriasis. Clinical studies published in English were considered; a total of four clinical trials met inclusion criteria. Studies were also evaluated by using the Jadad scale and Consort Statement in Reporting Clinical trials of Herbal Medicine Intervention. Quality and methodological accuracy of considered studies varied considerably, and some crucial information to reproduce clinical results was missing. We conclude that administration of aloe as cutaneous treatment is generally well tolerated, as no serious side effects were reported. Results on the effectiveness of Aloe vera are contradictory; our analysis reveals the presence of methodological gaps preventing to reach final conclusions. Copyright © 2015 John Wiley & Sons, Ltd.

Caída abrupta del tono muscular al entrar a sueño MOR en el ser humano

OpenAIRE

Rosales-Lagarde, Alejandra; del Río-Portilla, Irma Yolanda; Guevara, Miguel Ángel; Corsi-Cabrera, María

2009-01-01

De acuerdo con el manual estandarizado para la clasificación del sueño en el ser humano, tres variables fisiológicas marcan el inicio del sueño con movimientos oculares rápidos (MOR): la desincronización electroencefalográfica (EEG), los movimientos oculares rápidos y la pérdida de tono muscular. De estos tres indicadores, uno de ellos, los movimientos oculares rápidos, es una manifestación intermitente o fásica que consiste en movimientos que pueden ser aislados o emitirse en salvas de vario...

Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela

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María Eugenia Marquina

2011-09-01

Full Text Available Rasgos fenotípicos y marcadores moleculares de ADN se utilizaron para investigar la variación fenotípica y genética entre 12 aislados rizobianos venezolanos y 10 cepas de referencia internacionales. Para ello, se realizó un PCR-RFLP del gen rDNA 16S, la presencia de plásmidos grandes, análisis metabólicos en medios sólidos, resistencia a la salinidad, condiciones del crecimiento a diferentes pH y temperaturas y la resistencia intrínseca a antibióticos. En referencia a las cualidades fenotípicas, se diferenciaron tres grupos principales, un grupo I que abarcó a todas aquellas cepas que metabolizaban entre 67.5% y 90% de las fuentes de C y de N. También eran tolerantes a la acidez y productoras de ácido, capaces de crecer a 40ºC y a altas condiciones de salinidad (NaCl 2-2.5%. Con respecto a la sensibilidad a antibióticos, este grupo era susceptible a un 30% de los antibióticos empleados. Las cepas que pertenecen al grupo II exhibieron una tolerancia salina más baja (0.1- 1.5%NaCl, así como una menor tolerancia a la acidez, puesto que crecieron bien en valores de pH iguales o mayores a 5.0. Este grupo era resistente a todos los antibióticos probados y metabolizaban solamente entre 52.5%-82.5% de las fuentes de C y de N. Una sola cepa bacteriana representó al grupo III, con una baja tolerancia salina (0.1% NaCl. Este aislado creció a un pH mayor o igual a 5.6, era susceptible a 50% de los antibióticos probados y metabolizó el 72% de las fuentes de C y de N. Al tener como base el PCR-RFLP del 16S rDNA, se diferenciaron también tres grupos. Los miembros del grupo A demostraron una estrecha relación con Rhizobium tropici CiAT 899 y Sinorhizobium americanum CFN-Ei156, mientras que el grupo B está estrechamente vinculado a Bradyrhizobium spp. El grupo C, está representado por solo un aislado. El aislado Trebol, fue la única cepa capaz de formar nódulos y no aparece relacionado con ninguna cepa de referencia, y sugiere una

Penggunaan Gel Lidah Buaya (Aloe Vera) sebagai Koagulan Alami dalam Penjernihan Air Sumur di Desa Sausu Tambu Kecamatan Sausu

OpenAIRE

Mujariah, Mujariah; Abram, Paulus Hengky; Jura, Minarni Rama

2016-01-01

Research on the use of aloe vera gel (aloe vera) as a natural coagulant in water purification village wells Sausu Tambu has been done. This study aims to determine whether aloe vera gel can be used as a natural coagulant and determine the optimum ratio of aloe vera gel in water (V/V) which is used in the purification of water wells in the village Sausu Tambu. Research was conducted on the water sample preparation, natural production of aloe vera gel, water treatment and analysis of water qual...

Administration of dried Aloe vera gel powder reduced body fat mass in diet-induced obesity (DIO) rats.

Science.gov (United States)

Misawa, Eriko; Tanaka, Miyuki; Nabeshima, Kazumi; Nomaguchi, Kouji; Yamada, Muneo; Toida, Tomohiro; Iwatsuki, Keiji

2012-01-01

The aim of the present study was to investigate the anti-obesity effects of Aloe vera gel administration in male Sprague-Dawley (SD) rats with diet-induced obesity (DIO). SD rats at 7 wk of age were fed either a standard diet (10 kcal% fat) (StdD) or high-fat (60 kcal% fat) diet (HFD) during the experimental period. Four weeks after of HFD-feeding, DIO rats (11 wk of age) were orally administered with two doses of Aloe vera gel powder (20 and 200 mg/kg/d) for 90 d. Body weights (g) and body fat (%) of HFD fed rats were significantly higher than those of StdD-fed rats. Although a modest decrease of body weight (g) was observed with the administration of dried Aloe vera gel powder, both subcutaneous and visceral fat weight (g) and body fat (%) were reduced significantly in Aloe vera gel-treated rats. Serum lipid parameters elevated by HFD were also improved by the Aloe vera gel treatment. The oxygen consumption (VO(2)), an index of energy expenditure, was decreased in HFD-fed rats compared with that in StdD-fed rats. Administration of Aloe vera gel reversed the change in VO(2) in the HFD-fed rats. These results suggest that intake of Aloe vera gel reduced body fat accumulation, in part, by stimulation of energy expenditure. Aloe vera gel might be beneficial for the prevention and improvement of diet-induced obesity.

Effects of Aloe Sterol Supplementation on Skin Elasticity, Hydration, and Collagen Score: A 12-Week Double-Blind, Randomized, Controlled Trial.

Science.gov (United States)

Tanaka, Miyuki; Yamamoto, Yuki; Misawa, Eriko; Nabeshima, Kazumi; Saito, Marie; Yamauchi, Koji; Abe, Fumiaki; Furukawa, Fukumi

2016-01-01

Our previous study confirmed that Aloe sterol stimulates collagen and hyaluronic acid production in human dermal fibroblasts. This study aims to investigate whether Aloe sterol intake affects skin conditions. We performed a 12-week, randomized, double-blind, placebo-controlled study to evaluate the effects of oral Aloe sterol supplementation on skin elasticity, hydration, and the collagen score in 64 healthy women (age range 30-59 years; average 44.3 years) who were randomly assigned to receive either a placebo or an Aloe sterol-supplemented yogurt. Skin parameters were measured and ultrasound analysis of the forearm was performed. ANCOVA revealed statistical differences in skin moisture, transepidermal water loss, skin elasticity, and collagen score between the Aloe sterol and placebo groups. The gross elasticity (R2), net elasticity (R5), and biological elasticity (R7) scores of the Aloe sterol group significantly increased with time. In addition, skin fatigue area F3, which is known to decrease with age and fatigue, also increased with Aloe sterol intake. Ultrasound echogenicity revealed that the collagen content in the dermis increased with Aloe sterol intake. The results suggest that continued Aloe sterol ingestion contributes to maintaining healthy skin. © 2017 S. Karger AG, Basel.

Mechanism of Aloe Vera extract protection against UVA: shelter of lysosomal membrane avoids photodamage.

Science.gov (United States)

Rodrigues, Daniela; Viotto, Ana Cláudia; Checchia, Robert; Gomide, Andreza; Severino, Divinomar; Itri, Rosangela; Baptista, Maurício S; Martins, Waleska Kerllen

2016-03-01

The premature aging (photoaging) of skin characterized by wrinkles, a leathery texture and mottled pigmentation is a well-documented consequence of exposure to sunlight. UVA is an important risk factor for human cancer also associated with induction of inflammation, immunosuppression, photoaging and melanogenesis. Although herbal compounds are commonly used as photoprotectants against the harmful effects of UVA, the mechanisms involved in the photodamage are not precisely known. In this study, we investigated the effects of Aloe Vera (Aloe barbadensis mil) on the protection against UVA-modulated cell killing of HaCaT keratinocytes. Aloe Vera exhibited the remarkable ability of reducing both in vitro and in vivo photodamage, even though it does not have anti-radical properties. Interestingly, the protection conferred by Aloe Vera was associated with the maintenance of membrane integrity in both mimetic membranes and intracellular organelles. The increased lysosomal stability led to a decrease in lipofuscinogenesis and cell death. This study explains why Aloe Vera extracts offer protection against photodamage at a cellular level in both the UV and visible spectra, leading to its beneficial use as a supplement in protective dermatological formulations.

Aloe vera in active and passive regions of electronic devices towards a sustainable development

Science.gov (United States)

Lim, Zhe Xi; Sreenivasan, Sasidharan; Wong, Yew Hoong; Cheong, Kuan Yew

2017-07-01

The increasing awareness towards sustainable development of electronics has driven the search for natural bio-organic materials in place of conventional electronic materials. The concept of using natural bio-organic materials in electronics provides not only an effective solution to address global electronic waste crisis, but also a compelling template for sustainable electronics manufacturing. This paper attempts to provide an overview of using Aloe vera gel as a natural bio-organic material for various electronic applications. Important concepts such as responses of living Aloe vera plant towards electrical stimuli and demonstrations of Aloe vera films as passive and active regions of electronic devices are highlighted in chronological order. The biodegradability and biocompatibility of Aloe vera can bring the world a step closer towards the ultimate goal of sustainable development of electronic devices from "all-natural" materials.

Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model.

Science.gov (United States)

Lin, Li-Xin; Wang, Peng; Wang, Yu-Ting; Huang, Yong; Jiang, Lei; Wang, Xue-Ming

2016-02-01

Aloe vera and Vitis vinifera have been traditionally used as wound healing agents. The present study aimed to investigate the effects of aloe emodin and resveratrol in the burn wound healing procedure. Burn wounds are common in developed and developing countries, however, in developing countries, the incidence of severe complications is higher and financial resources are limited. The results of the present study demonstrated that neither aloe emodin or resveratrol were cytotoxic to THP-1 macrophages at concentrations of 1, 100 and 500 ng/ml. A significant increase in wound-healing activity was observed in mice treated with the aloe emodin and resveratrol, compared with those which received control treatments. The levels of IL-1β in the exudates of the burn wound area of the treated mice increased in a time-dependent manner over 7 days following burn wound injury. At 10 days post-injury, steady and progressive wound healing was observed in the control animals. The present study confirmed that increased wound healing occurs following treatment with aloe emodin,, compared with resveratrol, providing support for the use of Aloe vera plants to improve burn wound healing.

Obtención de Antraquinona contenida en el exudado de Aloe Vera (L.) Burm. f. (Zábila)

OpenAIRE

Saavedra, Oswaldo; Rondón, Carlos; Gallignani, Máximo; Ayala, Carlos; Nicola, Margarelli

2012-01-01

La investigación tuvo como propósito obtener la antraquinona contenida en el exudado de Aloe vera (L.) Burm.f. (zábila) mediante el método de cristalización y su identificación mediante la técnica de espectrofotometría de radiación infrarroja. La muestra la conformaron 18 plantas de zábila, recolectadas al oeste de la ciudad Santa Ana de Coro, estado Falcón. Se utilizaron tres métodos para la obtención de antraquinona a partir del exudado de zábila. En el método A, la antraquinona se obtuvo p...

Optimisation of nitrogen and potassium for Aloe vera (L.) Burm.f. in a ...

African Journals Online (AJOL)

Aloe vera (L.) Burm.f. is highly appreciated due to its short growth period and high economic value among all Aloe species, and is used in pharmaceuticals, folk medicine, healthcare, cosmetic products and food products. Hydroponic systems have the potential to improve the cropping management and to achieve higher ...

EVALUACIÓN DEL ANTAGONISMO Y MULTIPLICACIÓN DE Trichoderma sp. EN SUSTRATO DE PLÁTANO EN MEDIO LÍQUIDO ESTÁTICO

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Elkin Agamez Ramos

2009-09-01

Full Text Available Se evaluó la capacidad antagónica de nueve aislados del hongo Trichoderma sp. proveniente de la colección de cepas del Laboratorio de Fitopatología de la Universidad de Córdoba, Colombia y su producción de esporas en medios de cultivo líquido estático, a través de pruebas in vitro de antagonismo, utilizando dos hongos fitopatógenos: Fusarium sp. y Rhizoctonia sp.; esta prueba permitió seleccionar el aislado con la mayor actividad antagónica, para así ajustar la producción de esporas mediante un proceso de fermentación en estado líquido en condición estática mediante procesos de buferización y sin buferización con fosfato 0,2N, utilizándose como sustrato principal harina de plátano (HP 5%, además de complementos nutricionales que constituyeron los tratamientos: HPM (solución de melaza 5%, HPL (solución de levadura granulada al 2%, HPU (solución de urea 0,1%, y como testigo PGL (papa glucosa líquida. En los ensayos de antagonismo se seleccionó el aislado C2 de Trichoderma sp. por presentar la mayor capacidad antagónica frente al hongo Fusarium sp., con un porcentaje de inhibición del 78,30% y reportando un índice 4 de antagonismo, en los procesos de producción de esporas el tratamiento HPL (harina de plátano más levadura sin buffer se destacó por su favorable producción de conidias, con una concentración de 1,1 x 109 conidias/mL. Los procesos de buferización se diferenciaron significativamente, demostrando que la variable buffer influyó en la producción de conidias, los medios que se amortiguaron mostraron una producción de 1,01 x 109 conidias/mL, con respecto a los que no se amortiguaron 6,3 x 108 conidias/mL.

Aloe vera attenuated gastric injury on indomethacin-induced gastropathy in rats.

Science.gov (United States)

Werawatganon, Duangporn; Rakananurak, Narisorn; Sallapant, Sasipim; Prueksapanich, Piyapan; Somanawat, Kanjana; Klaikeaw, Naruemon; Rerknimitr, Rungsun

2014-12-28

To evaluate the protective effects of Aloe vera on gastric injury in rats with indomethacin (IMN)-induced gastropathy. Male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control, n = 6) was given distilled water (DW) orally. Group 2 (IMN, n = 6) was given oral IMN (150 mg/kg) dissolved in 5% sodium bicarbonate (NaHCO3 (-)) at time 0 and 4 h. Group 3 (Aloe vera-treated, n = 6) was given oral Aloe vera (150 mg/kg) dissolved in DW and IMN at time 0 and 4 h. Eight hours later, the stomach was removed to determine gastric malondialdehyde (MDA), the number of interleukin (IL)-18 positive stained cells (%) by immunohistochemistry, and for histopathological examination. Then, the serum was collected to determine tumor necrosis factor (TNF)-α and cytokine-induced neutrophil chemoattractant (CINC)-1 by sandwich enzyme linked immunosorbent assay method. In the IMN group, serum TNF-α, CINC-1 and gastric MDA were significantly increased when compared to the control group (27.78 ± 1.52 pg/mL vs 85.07 ± 49.11 pg/mL, P = 0.009; 104.55 ± 45.80 pg/mL vs 1054.70 ± 20.38 pg/mL, and 1.74 ± 0.21 nmol/mg vs 9.36 ± 1.07 nmol/mg protein, P = 0.000, respectively). The mean level of TNF-α, CINC-1 and gastric MDA in the Aloe vera-treated group were improved as compared with the IMN group (85.07 ± 49.11 pg/mL vs 35.19 ± 1.61 pg/mL, P = 0.021; 1054.70 ± 20.38 pg/mL vs 813.56 ± 239.04 pg/mL, P = 0.025; and 9.36 ± 1.07 nmol/mg vs 2.67 ± 0.64 nmol/mg protein, P = 0.000, respectively). The number of IL-18 positive stained cells (%) in the gastric epithelial cells of the IMN group was significantly higher than the control group (5.01% ± 3.73% vs 30.67% ± 2.03%, P = 0.000, respectively). In contrast, Aloe vera treatment decreased the number of IL-18 positive stained cells (%) significantly when compared with the IMN group (30.67% ± 2.03% vs 13.21% ± 1.10%, P = 0.000, respectively). Most rats in the IMN group developed moderate to severe gastric inflammation

Continuous processing of Aloe Vera juice in Reverse Osmosis integrated plant

International Nuclear Information System (INIS)

Nasim, H.; Younas, M.; Feroz, N.; Swati, I.K.

2012-01-01

Membrane technology is being applied in the food and beverages industry particularly in fruit juice concentration all over the world. The major advantages are lesser use of energy, better taste of products, and recovery of pure aroma/flavor and ease of operation. The current study is focused on the experimental investigation of clarification and concentration of Aloe juice through membrane separation technique. The experimental procedure consists of Aloe gel followed by pulping, a clarification by filtration and the concentration by reverse osmosis (RO). Experimental rig was integrated with spiral wound TFM-50 membrane, pre-treatment filters, pumps, rota meter and pressure sensors. The effect of feed pressure and temperature was studied on the dynamic behavior of RO integrated plant for water removal and permeate flux. It was found that Aloe juice was concentrated at optimum pressure and temperature of 40 bar and 40 degree C, respectively. (author)

Effects of the application of Aloe vera (L. and microcurrent on the healing of wounds surgically induced in Wistar rats Efeitos da aplicação de Aloe vera (L. e microcorrente no reparo de lesões cirúrgicas induzidas em ratos Wistar

Directory of Open Access Journals (Sweden)

Cristina Cruz Franchini

2009-04-01

Full Text Available PURPOSE: To investigate the effects of topical application of an Aloe vera gel combined or not with microcurrent application on the healing of skin wounds surgically induced in Wistar rats. METHODS: The animals were randomly divided into the following groups: control group, animals topically treated with Aloe vera, animals treated with a microcurrent, and animals receiving topical application of Aloe vera combined with microcurrent application. RESULTS: The results indicated differences in wound healing between the various treatments when compared to the control group. Tissue hyperplasia was lower in the control group compared to the other treated groups. Accelerated wound healing was observed in the group treated with Aloe vera compared to control. Animals submitted to microcurrent application only and the group treated with microcurrent plus Aloe vera presented an earlier onset of the proliferative phase compared to the control group and animals treated with Aloe vera gel alone. Morphometric data confirmed the structural findings. CONCLUSION: Simultaneous application of Aloe vera gel and microcurrent is an excellent choice for the treatment of open wounds thus indicating a synergistic action of these two applications.OBJETIVO: Investigar os efeitos da aplicação tópica do gel de Aloe vera, combinada ou não com a aplicação de microcorrente no reparo de lesões cutâneas induzidas cirurgicamente em ratos Wistar. MÉTODOS: Os animais foram distribuídos aleatoriamente em: grupo controle, tratado topicamente com gel in natura de Aloe vera, tratado com microcorrente e tratado com aplicação tópica de Aloe vera associada à microcorrente. RESULTADOS: Os resultados do presente trabalho indicaram que o reparo tecidual ocorreu de forma diferenciada nos vários tratamentos empregados quando comparados ao grupo controle. A hiperplasia tecidual no grupo controle foi menor que a observada nos demais grupos tratados. No grupo tratado com aplica

In-vitro assessment and pharmacodynamics of nimesulide incorporated Aloe vera transemulgel.

Science.gov (United States)

Vandana, K R; Yalavarthi, Prasanna R; Sundaresan, C R; Sriramaneni, Raghava N; Vadlamudi, Harini C

2014-06-01

The aim of the investigation was to prepare nimesulide emulsion for incorporation in Aloe vera gel base to formulate 'nimesulide - Aloe vera transemulgel' (NAE) and to carryout in-vitro assessment and in-vivo anti-inflammatory studies of the product. Although the use of nimesulide is banned for oral administration, due to its potential for inducing hepatotoxicity and thrombocytopenia, the use of nimesulide for topical delivery is prominent in the treatment of many inflammatory conditions including rheumatoid arthritis. The drug loading capacity of transdermal gels is low for hydrophobic drugs such as nimesulide. Nimesulide can be effectively incorporated into emulgels (a combination of emulsion and gel). Aloe vera has a mild anti-inflammatory effect and in the present study Aloe vera gel was formulated and used as a gel base to prepare NAE. The emulgels thus prepared were evaluated for viscosity, pH, in-vitro permeation, stability and skin irritation test. In-vivo anti-inflammatory studies were performed using carrageenan induced hind paw edema method in Wistar rats. The results were compared with that of commercial nimesulide gel (CNG). From the in-vitro studies, effective permeation of nimesulide from NAE (53.04 %) was observed compared to CNG (44.72 %) at 30 min indicating better drug release from NAE. Topical application of the emulgel found no skin irritation. Stability studies proved the integrity of the formulation. The percentage of inhibition of edema was highest for the prepared NAE (67.4 % inhibition after 240 min) compared to CNG (59.6 %). From our results, it was concluded that the Aloe vera gel acts as an effective gel base to prepare nimesulide emulgel with high drug loading capacity (86.4 % drug content) compared to CNG (70.5 % drug content) with significant anti-inflammatory effect.

Acute toxic hepatitis caused by an aloe vera preparation in a young patient: a case report with a literature review.

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Lee, Jeonghun; Lee, Mi Sun; Nam, Kwan Woo

2014-07-01

Aloe is one of the leading products used in phytomedicine. Several cases of aloe-induced toxic hepatitis have been reported in recent years. However, its toxicology has not yet been systematically described in the literature. A 21-year-old female patient was admitted to our hospital with acute hepatitis after taking an aloe vera preparation for four weeks. Her history, clinical manifestation, laboratory findings, and histological findings all led to the diagnosis of aloe vera-induced toxic hepatitis. We report herein on a case of acute toxic hepatitis induced by aloe vera.

DOCUMENTO: FORMAS DE EVALUACIÓN DEL APRENDIZAJE

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Marcela Currea Galvis

2004-08-01

Full Text Available Las notas de seguimiento (usadas por casi todos los docentes y educadores se constituyen en lo que se conoce (en muchas instituciones cómo evaluaciones parciales y finales, y permiten utilizar diferentes tipos y esquemas para laevaluación del aprendizaje y del desarrollo de competencias, es decir, la articulacióny uso de saberes, las formas de razonar y proceder para comprender situaciones, solucionar problemas y hacer en contexto, lográndose con aquéllas notas establecer diferenciaciones significativas de los múltiples procesos y conductas pertenecientes alos campos cognoscitivo, afectivo y psicomotor de cada uno de los estudiantes.

Active listening in medical consultations: development of the Active Listening Observation Scale (ALOS-global).

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Fassaert, Thijs; van Dulmen, Sandra; Schellevis, François; Bensing, Jozien

2007-11-01

Active listening is a prerequisite for a successful healthcare encounter, bearing potential therapeutic value especially in clinical situations that require no specific medical intervention. Although generally acknowledged as such, active listening has not been studied in depth. This paper describes the development of the Active Listening Observation Scale (ALOS-global), an observation instrument measuring active listening and its validation in a sample of general practice consultations for minor ailments. Five hundred and twenty-four videotaped general practice consultations involving minor ailments were observed with the ALOS-global. Hypotheses were tested to determine validity, incorporating patients' perception of GPs' affective performance, GPs' verbal attention, patients' self-reported anxiety level and gender differences. The final 7-item ALOS-global had acceptable inter- and intra-observer agreement. Factor analysis revealed one homogeneous dimension. The scalescore was positively related to verbal attention measured by RIAS, to patients' perception of GPs' performance and to their pre-visit anxiety level. Female GPs received higher active listening scores. The results of this study are promising concerning the psychometric properties of the ALOS-global. More research is needed to confirm these preliminary findings. After establishing how active listening differentiates between health professionals, the ALOS-global may become a valuable tool in feedback and training aimed at increasing listening skills.

Could We Really Use Aloe vera Food Supplements to Treat Diabetes? Quality Control Issues

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Solomon Habtemariam

2017-01-01

Full Text Available Diabetes UK has recently listed a number of herbs and spices that have been clinically shown to improve blood glucose control in type-2 diabetes patients and the diabetes high-risk group. With Aloe vera being top in this list, its health benefit along with health and beauty/food retailers supplying it was illustrated in detail. Previous article from this laboratory scrutinised the merit of using A. vera as an alternative therapy to prescription antidiabetic drugs and the risk of using food supplements in the market which do not qualify as drug preparations. In continuation of this discussion, the present study assesses three Aloe Pura brands and one Holland and Barret brand of A. vera juice supplements in the UK market through chromatographic and spectroscopic analysis. While the polysaccharide active ingredient, acemannan, appears to be within the recommended limit, it was found that Aloe Pura (one of the best-selling brands for A. vera supplement products have benzoate additive that does not appear in the supplement levels. Moreover, two of the Aloe Pura brand juices contain methanol, suggesting that the International Aloe Science Council (IASC certification does not guarantee the medicinal quality of these products. The therapeutic fitness of such supplements is discussed.

Could We Really Use Aloe vera Food Supplements to Treat Diabetes? Quality Control Issues

Science.gov (United States)

2017-01-01

Diabetes UK has recently listed a number of herbs and spices that have been clinically shown to improve blood glucose control in type-2 diabetes patients and the diabetes high-risk group. With Aloe vera being top in this list, its health benefit along with health and beauty/food retailers supplying it was illustrated in detail. Previous article from this laboratory scrutinised the merit of using A. vera as an alternative therapy to prescription antidiabetic drugs and the risk of using food supplements in the market which do not qualify as drug preparations. In continuation of this discussion, the present study assesses three Aloe Pura brands and one Holland and Barret brand of A. vera juice supplements in the UK market through chromatographic and spectroscopic analysis. While the polysaccharide active ingredient, acemannan, appears to be within the recommended limit, it was found that Aloe Pura (one of the best-selling brands for A. vera supplement) products have benzoate additive that does not appear in the supplement levels. Moreover, two of the Aloe Pura brand juices contain methanol, suggesting that the International Aloe Science Council (IASC) certification does not guarantee the medicinal quality of these products. The therapeutic fitness of such supplements is discussed. PMID:29511381

Encapsulation of Aloe Vera and Its Effect During Yogur Incubation

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Ricardo Adolfo Parra Huertas

2014-11-01

Full Text Available The yogurt is milk derivative highly consumed around the world,as well as aloe vera. Both have reports tocontribute to human health. The purpose of this research is to determine the effect of the addition of capsules with aloe vera during the incubation of yogurt. Aloeverawas encapsulated in alginate at two different concentrations, 1% and 2%,addingthe capsules from the moment of incubation and comparing the effect of the addition of capsules withthe non-addition of them. For these samples were determined: pH, acidity, syneresis, lactic acid bacteria count, sensory evaluation and proximate analysis. The results indicated that for the three treatments pH values and acid behaved similarly to each characteristic of the yogurt during incubation. The lactic acid bacteria count indicated that treatment with capsules containing 2% sodium alginate had higher counts. Sensorially, three treatments had a favorable acceptability; proximate analysis had favorable values . In conclusion,the tests showed the viability of encapsulated aloe vera in the manufacture of yogurt during incubation time without being affected by the concentration of sodium alginate.

Soluble epoxide hydrolase inhibitory activity of anthraquinone components from Aloe.

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Sun, Ya Nan; Kim, Jang Hoon; Li, Wei; Jo, A Reum; Yan, Xi Tao; Yang, Seo Young; Kim, Young Ho

2015-10-15

Aloe is a short-stemmed succulent herb widely used in traditional medicine to treat various diseases and as raw material in cosmetics and heath foods. In this study, we isolated and identified two new anthraquinone derivatives, aloinoside C (6) and aloinoside D (7), together with six known compounds from an aqueous dissolved Aloe exudate. Their structures were identified by spectroscopic analysis. The inhibitory effects of the isolated compounds on soluble epoxide hydrolase (sEH) were evaluated. Compounds 1-8 inhibited sEH activity potently, with IC50 values ranging from 4.1±0.6 to 41.1±4.2 μM. A kinetic analysis of compounds 1-8 revealed that the inhibitory actions of compounds 1, 6 and 8 were non-competitive, whereas those of compounds 2-5 and 7 were the mixed-type. Molecular docking increases our understanding of receptor-ligand binding of all compounds. These results demonstrate that compounds 1-8 from Aloe are potential sEH inhibitors. Copyright © 2015 Elsevier Ltd. All rights reserved.

Effect of salicylic acid and aloe vera gel on postharvest quality of table grapes ( Vitis Vinifera

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H. Peyro

2017-06-01

Full Text Available To investigate the effects of salicylic acid dipping and Aloe vera gel coating on shelf life and post harvest quality of table grapes (Vitis vinifera of the cultivar Shahroudi, a factorial experiment was conducted on the basis of randomized complete blocks design with three factors and three replicates in agricultural faculty of Islamic Azad University in 2014. The treatments were dipping in Salicylic acid (three levels of 0, 1 and 2 mmmol-1 for 15 minutes and coating with Aloe vera gel (four levels of 0, 10%, 15% and 20% w/v and measurement of traits in 1st day, 30th day and 60th day after treatment of berries. The results showed that the interaction effect of salicylic acid and Aloe vera gel application was significant on all of traits except for pH value in a way that the best and the minimum weight loss (0.09g was obtained by application of 2 mmol-1 Salicylic acid and 20% Aloe vera gel in 1st day after treatment. The greatest amount of total soluble solids (428.43 g.100g-1 fruit juice was found in 2 mmol-1 Salicylic acid and 15% Aloe vera gel in 60th day. The highest Catalase enzyme activity (0.0013 Ua.mg-1Pro was attained in 2 mmol-1 Salicylic acid and 15% Aloe vera gel in 30th day. These results demonstrated that treatment of grape berries by salicylic acid and Aloe vera gel had positive effect on shelf life of table grapes and their postharvest quality

Effects of Aloe vera on dressing percentage and haemato-biochemidal parameters of broiler chickens

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Jagmohan Singh

2013-09-01

Full Text Available Aim: To evaluate the effects of Aloe vera on dressing percentage and hemato-biochemical parameters of broiler chickens.Materials and Methods: A total of 90 chicks were used in this study. They were randomly allocated into 3 treatment groups.Fresh Aloe vera leaf juice (ALJ was prepared and administered to the test group T3 at the rate of 20 g/Lin drinking water daily.This study was carried out for 42 days. Dressing percentage and hemato-biochemical parameters were recorded at the end ofexperiment.Results: Group that was given Aloe vera (T3 showed numerically higher dressing percentage as compared to control group(T1 and drug control group (T2. It also showed significantly (P0.05 differences were observed in other parameters among all the treatment groups.Conclusion: Aloe vera has potential to be a growth promoter in broiler chicks and its growth promoting effects are comparableto that of antibiotic growth promoter (AGP.

Anti-tumor activity of Aloe vera against DMBA/croton oil-induced skin papillomagenesis in Swiss albino mice.

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Saini, M; Goyal, Pradeep Kumar; Chaudhary, Geeta

2010-01-01

Human populations are increasingly exposed to various carcinogens such as chemicals, radiation, and viruses in the environment. Chemopreventive drugs of plant origin are a promising strategy for cancer control because they are generally nontoxic or less toxic than synthetic che-mopreventive agents, and can be effective at different stages of carcinogenesis. The present investigation was undertaken to explore the antitumor activity of topical treatment with aloe vera (Aloe vera) gel, oral treatment with aloe vera extract, and topical and oral treatment with both gel and extract in stage-2 skin carcinogenesis in Swiss albino mice induced by 7,12-dim ethylbenz(a)anthracene (DMBA) and promoted croton (Croton tiglium) oil. The animals were randomly divided into 4 groups and treated as follows: Group I, DMBA + croton oil only (controls); Group II, DMBA + croton oil + topical aloe vera gel; Group III, DMBA + croton oil + oral aloe vera extract; Group I V, DMBA + croton oil + topical aloe vera gel + oral aloe vera extract. Results showed that body weight was significantly increased from 78.6% in the control group (Group I) to 92.5%, 87.5%, and 90.0% in Groups II, III, and I V, respectively. A 100% incidence of tumor development was noted in Group I, which was decreased to 50%, 60%, and 40% in Groups II, III, and I V, respectively. Also in Groups II, III, and IV, the cumulative number of papillomas was reduced significantly from 36 to 12, 15, and 11; tumor yield from 3.6 to 1.2, 1.5, and 1.1; and tumor burden from 3.6 to 2.4, 2.50, and 2.75, respectively, after treatment with aloe vera. Conversely, the average latent period increased significantly from 4.9 (Group I) to 5.23, 5.0, and 6.01 weeks in Groups II, III, and I V, respectively. We conclude that aloe vera protects mice against DMBA/croton oil-induced skin papillomagenesis, likely due to the chemopreventive activity of high concentrations of antioxidants such as vitamins A, C, and E; glutathione peroxidase; several

Release Behavior and Antibacterial Activity of Chitosan/Alginate Blends with Aloe vera and Silver Nanoparticles.

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Gómez Chabala, Luisa Fernanda; Cuartas, Claudia Elena Echeverri; López, Martha Elena Londoño

2017-10-24

Aloe vera is a perennial plant employed for medical, pharmaceutical and cosmetic purposes that is rich in amino acids, enzymes, vitamins and polysaccharides, which are responsible for its therapeutic properties. Incorporating these properties into a biopolymer film obtained from alginate and chitosan allowed the development of a novel wound dressing with antibacterial capacity and healing effects to integrate the antibacterial capacity of silver nanoparticles with the healing and emollient properties of Aloe vera gel. Three alginate-chitosan matrices were obtained through blending methods using different proportions of alginate, chitosan, the Aloe vera (AV) gel and silver nanoparticles (AgNps), which were incorporated into the polymeric system through immersion methods. Physical, chemical and antibacterial characteristics were evaluated in each matrix. Interaction between alginate and chitosan was identified using the Fourier transform infrared spectroscopy technique (FTIR), porosity was studied using scanning electron microscopy (SEM), swelling degree was calculated by difference in weight, Aloe vera gel release capacity was estimated by applying a drug model (Peppas) and finally antibacterial capacity was evaluated against S. Aureus and P. aeruginosa . Results show that alginate-chitosan (A (1:3 Chit 1/Alg 1); B (1:3 Chit 1.5/Alg 1) and C (3:1 Chit 1/Alg 1/B12)) matrices with Aloe vera (AV) gel and silver nanoparticles (AgNps) described here displayed antibacterial properties and absorption and Aloe vera release capacity making it a potential wound dressing for minor injuries.

The combined effects of Aloe vera gel and silver nanoparticles on wound healing in rats

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Y. Yousefpoor

2016-01-01

Full Text Available Objective(s: This study was aimed at investigating the synergy effects of Aloe vera gel and silver nanoparticles on the healing rate of the cutting wounds. Materials and Methods: In order to determine the concentration of silver nanoparticles in Aloe vera gel, the MBC methods were applied on the most common bacteria infecting wounds, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The cutting wounds with Full-thickness skin were dorsally created on rats; then the rats were divided into 4 groups. The treatments groups included: mixture of Aloe vera gel and silver nanoparticles, Aloe vera gel alone and silver nanoparticles alone in addition to control groups. The treatment was carried out for 2 weeks and the size of the wound closures were measured by an image software analysis. Results:There was no significant difference (p

Aloe vera gel improves behavioral deficits and oxidative status in streptozotocin-induced diabetic rats.

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Tabatabaei, Seyed Reza Fatemi; Ghaderi, Shahab; Bahrami-Tapehebur, Mohammad; Farbood, Yaghoob; Rashno, Masome

2017-12-01

Oxidative stress has a major role in progression of diabetes-related behavioral deficits. It has been suggested that Aloe vera has anti-diabetic, antioxidative, and neuroprotective effects. The present study was designed to determine the effects of Aloe vera gel on behavioral functions, oxidative status, and neuronal viability in the hippocampus of streptozotocin (STZ)-induced diabetic rats. Fifty five adult male Wistar rats were randomly divided into five groups, including: control (normal saline 8ml/kg/day; P.O.), diabetic (normal saline 8ml/kg/day; P.O.), Aloe vera gel (100mg/kg/day; P.O.), diabetic+Aloe vera gel (100mg/kg/day; P.O.) and diabetic+NPH insulin (10 IU/kg/day; S.C.). All treatments were started immediately following confirmation of diabetes in diabetic groups and were continued for eight weeks. Behavioral functions were evaluated by employing standard behavioral paradigms. Additionally, oxidative status and neuronal viability were assessed in the hippocampus. The results of behavioral tests showed that diabetes enhanced anxiety/depression-like behaviors, reduced exploratory and locomotor activities, decreased memory performance, and increased stress related behaviors. These changes in diabetic rats were accompanied by increasing oxidative stress and neuronal loss in the hippocampus. Interestingly, eight weeks of treatment with Aloe vera gel not only alleviated all the mentioned deficits related to diabetes, but in some aspects, it was even more effective than insulin. In conclusion, the results suggest that both interrelated hypoglycemic and antioxidative properties of Aloe vera gel are possible mechanisms that improve behavioral deficits and protect hippocampal neurons in diabetic animals. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Facile fabrication of aloe vera containing PCL nanofibers for barrier membrane application.

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Carter, Princeton; Rahman, Shekh M; Bhattarai, Narayan

2016-01-01

Guided tissue regeneration (GTR) is a widely used method in dental surgical procedures that utilizes a barrier membrane to exclude migration of epithelium and ensure repopulation of periodontal ligament cells at the sites having insufficient gingiva. Commercial GTR membranes are typically composed of synthetic polymers that have had mild clinical success mostly because of their lack of proper bioactivity and appropriate degradation profile. In this study, a natural polymer, aloe vera was blended with polycaprolactone (PCL) to create nanofibrous GTR membranes by electrospinning. Aloe vera has proven anti-inflammatory properties and enhances the regeneration of periodontium tissues. PCL, a synthetic polymer, is well known to produce miscible polyblends nanofibers with natural polymers. Nanofibrous membranes with varying composition of PCL to aloe vera were fabricated, and several physicochemical and biological properties, such as fiber morphology, wettability, chemical structure, mechanical strength, and cellular compatibility of the membranes were analyzed. PCL/aloe vera membranes with ratios from 100/00 to 70/30 showed good uniformity in fiber morphology and suitable mechanical properties, and retained the integrity of their fibrous structure in aqueous solutions. Experimental results, using cell viability assay and cell attachment observation, showed that the nanofibrous membranes support 3T3 cell viability and could be a potential candidate for GTR therapy.

Precipitation of hydroxyapatite on electrospun polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds for bone tissue engineering.

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Shanmugavel, Suganya; Reddy, Venugopal Jayarama; Ramakrishna, Seeram; Lakshmi, B S; Dev, Vr Giri

2014-07-01

Advances in electrospun nanofibres with bioactive materials have enhanced the scope of fabricating biomimetic scaffolds for tissue engineering. The present research focuses on fabrication of polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds by electrospinning followed by hydroxyapatite deposition by calcium-phosphate dipping method for bone tissue engineering. Morphology, composition, hydrophilicity and mechanical properties of polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds along with controls polycaprolactone and polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds were examined by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle and tensile tests, respectively. Adipose-derived stem cells cultured on polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds displayed highest cell proliferation, increased osteogenic markers expression (alkaline phosphatase and osteocalcin), osteogenic differentiation and increased mineralization in comparison with polycaprolactone control. The obtained results indicate that polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds have appropriate physico-chemical and biological properties to be used as biomimetic scaffolds for bone tissue regeneration. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Millimeter Detection Of AlO (X2Σ+) In The Oxygen-rich Envelope Of VY Canis Majoris

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Tenenbaum, Emily D.; Ziurys, L. M.

2009-05-01

A new circumstellar molecule, the radical AlO (X 2Σ+), has been detected toward the envelope of the oxygen-rich supergiant star VY Canis Majoris (VY CMa) via three rotational emission lines. The N = 7 → 6 and 6 → 5 features of AlO were observed at 1 mm using the Arizona Radio Observatory Submillimeter Telescope (ARO SMT) and the N = 4 → 3 line was detected at 2 mm using the ARO 12 m dish. All lines exhibit noticeable hyperfine broadening due to the I = 5/2 spin of the aluminum nucleus. Based on simulations of the line profiles, AlO most likely arises from the dust-acceleration zone in the spherical outflow of VY CMa, with a source size of θs 0.5''. Given this source size, the column density of AlO was found to be Ntot 2 × 1015 cm-2 for Trot 230 K, with a fractional abundance, relative to H2, of 10-8. Gas-phase thermodynamic equilibrium chemistry is the likely formation mechanism for AlO in VY CMa, but shocks may disrupt the condensation process into Al2O3, allowing AlO to survive 20 stellar radii. The detection of AlO in VY CMa is additional evidence of an active gas-phase refractory chemistry in oxygen-rich envelopes, and suggests such objects may be fruitful sources for other new oxide identifications.

Preliminary antiplaque efficacy of aloe vera mouthwash on 4 day plaque re-growth model: randomized control trial.

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Gupta, Rajendra Kumar; Gupta, Devanand; Bhaskar, Dara John; Yadav, Ankit; Obaid, Khursheed; Mishra, Sumit

2014-04-01

Due to increasing resistance to antibiotics and rising incidence of oral diseases, there is a need for alternative treatment modalities to combat oral diseases. The aim of the present study was to access the effect of Aloe vera mouthwash on the dental plaque in the experimental period of 4 days and to compare it with the bench mark control chlorhexidine and placebo (saline water). A total of 300 systemically healthy subjects were randomly allocated into 3 groups: Aloe vera mouthwash group (n=100), control group (=100)-chlorhexidene group and saline water-Placebo (n=100). To begin with, Gingival index (GI) and plaque index (PI) were recorded. Then, baseline plaque scores were brought to zero by professionally cleaning the teeth with scaling and polishing. After randomization of the participants into three groups they were refrained from regular mechanical oral hygiene measures. Subjects were asked to swish with respective mouthwash (Aloe vera mouthwash, 0.2%chlorhexidine gluconate mouthwash, or normal saline) as per therapeutic dose for 4 days. The results showed that Aloe vera mouthrinse is equally effective in reducing plaque as Chlorhexidine compared to placebo over a period of 4 days. There was a significant reduction on plaque in Aloe vera and chlorhexidine groups and no statistically significant difference was observed among them (p>0.05). Aloe vera mouthwash showed no side effects. The results of the present study indicated that Aloe vera may prove an effective mouthwash due to its ability in reducing dental plaque.

UJI AKTIVITAS KOMBINASI EKSTRAK AIR LIDAH BUAYA ( Aloe vera L.) DAN AKAR MANIS (Glycyrrhiza glabra L.) SEBAGAI PENYUBUR RAMBUT

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Sulistiorini Indriaty; Teti Indrawati; Shelly Taurhesia

2016-01-01

Aloe vera (Aloe vera L.) contains amino acids, vitamins, folic acid, and liquorice (Glycyrrhiza glabra L) containing glisirizin, and flavonoids, which have properties as fertilizer hair. This study aims to obtain a combination of extracts with activity most optimal fertilizer hair, made 3 combination with a combination of 1 (the water extract of liquorice concentration of 2.5% and 7.5% aloe vera), a combination of 2 (the water extract of liquorice concentrations of 2, 5% and 2.5% aloe vera), ...

GONÇALO M. TAVARES’S POETIC MATERIALITY

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Paloma Roriz Espínola

2015-11-01

Full Text Available It is not rare that arquitecture opens up to dialog with literature, like it is clearly noticed with the appropriation that some writers have done with it. Such as with Paul Valery’s and João Cabral de Melo Neto’s with their recria­tion of Amphion’s myth. The anglo-portuguese writer Gonçalo M. Tavares argues on two essays, Arquitetura, natureza e amor and A estranha casa do senhor Walser, an approximation of the duties of a writer and of an arqui­tect. The purpose of this essay is to demonstrate how Gonçalo M. Tavares’ approach reflects on his body of work which is based on the search of the material aspect of language. More especifically on the poems that compose his book entitled 1, a body of work which attempts to articulate ideas with the “emotional lucidity” of Roland Barthes and the “motion” of Georges Didi-Huberman.

Tipificación fágica de aislados de Salmonella enteritidis de muestras clínicas, alimentarias y avícolas en Chile Phage typing of Salmonella enteritidis isolates from clinical, food, and poultry samples in Chile

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Soledad Prat

2001-01-01

Full Text Available Desde 1994 Chile ha sido afectado por una extensa epidemia de infecciones por Salmonella enteritidis (S. enteritidis. Para conocer la diversidad de fuentes infectantes, el posible origen de la epidemia y las relaciones epidemiológicas entre aislados clínicos, alimentarios y avícolas, se realizó la tipificación fágica de 310 muestras de S. enteritidis de origen clínico recolectadas entre los años 1975 y 1996 junto con 47 aislados alimentarios obtenidos durante brotes de S. enteritidis y 27 cepas aisladas en estudios de vigilancia de establecimientos de avicultura. En total, en las muestras clínicas se identificaron 13 fagotipos, dos aislados no pudieron tipificarse y uno se consideró atípico. Los tipos fágicos que se identificaron con mayor frecuencia fueron el 1 (56,8% y el 4 (31,3%, seguidos de los tipos 8 (4,8% y 28 (1,9%. La presencia regional y temporal de estos fagotipos indica que ha habido cambios importantes en su distribución: en los primeros años de recolección solo se registraron los fagotipos 8 y 28, que desaparecieron hacia 1980 para reaparecer de forma aislada en 1996. A partir de la expansión paulatina de S. enteritidis que se inició en 1988, se empezaron a aislar en las zonas central y sur del país fagos del tipo 4, que no se habían encontrado anteriormente en Chile. En 1991 emergió como predominante en la zona norte el fagotipo 1, tampoco registrado previamente. En los aislados alimentarios se identificaron solamente los tipos 1 y 4, que fueron también los más comunes en los aislados avícolas. La tipificación fágica de S. enteritidis es de utilidad para orientar el análisis epidemiológico de las infecciones por este agente patológico.Since 1994 an extensive epidemic of infections with Salmonella enteritidis (S. enteritidis has affected Chile. In order to understand the diversity of infective sources, the possible origin of the epidemic, and the epidemiological relationships between clinical, food, and

Microwave-assisted fibrous decoration of mPE surface utilizing Aloe vera extract for tissue engineering applications.

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Balaji, Arunpandian; Jaganathan, Saravana Kumar; Supriyanto, Eko; Muhamad, Ida Idayu; Khudzari, Ahmad Zahran Md

2015-01-01

Developing multifaceted, biocompatible, artificial implants for tissue engineering is a growing field of research. In recent times, several works have been reported about the utilization of biomolecules in combination with synthetic materials to achieve this process. Accordingly, in this study, the ability of an extract obtained from Aloe vera, a commonly used medicinal plant in influencing the biocompatibility of artificial material, is scrutinized using metallocene polyethylene (mPE). The process of coating dense fibrous Aloe vera extract on the surface of mPE was carried out using microwaves. Then, several physicochemical and blood compatibility characterization experiments were performed to disclose the effects of corresponding surface modification. The Fourier transform infrared spectrum showed characteristic vibrations of several active constituents available in Aloe vera and exhibited peak shifts at far infrared regions due to aloe-based mineral deposition. Meanwhile, the contact angle analysis demonstrated a drastic increase in wettability of coated samples, which confirmed the presence of active components on glazed mPE surface. Moreover, the bio-mimic structure of Aloe vera fibers and the influence of microwaves in enhancing the coating characteristics were also meticulously displayed through scanning electron microscopy micrographs and Hirox 3D images. The existence of nanoscale roughness was interpreted through high-resolution profiles obtained from atomic force microscopy. And the extent of variations in irregularities was delineated by measuring average roughness. Aloe vera-induced enrichment in the hemocompatible properties of mPE was established by carrying out in vitro tests such as activated partial thromboplastin time, prothrombin time, platelet adhesion, and hemolysis assay. In conclusion, the Aloe vera-glazed mPE substrate was inferred to attain desirable properties required for multifaceted biomedical implants.

Aloe vera attenuated liver injury in mice with acetaminophen-induced hepatitis.

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Werawatganon, Duangporn; Linlawan, Sittikorn; Thanapirom, Kessarin; Somanawat, Kanjana; Klaikeaw, Naruemon; Rerknimitr, Rungsun; Siriviriyakul, Prasong

2014-07-08

An overdose of the acetaminophen causes liver injury. This study aims to examine the anti-oxidative, anti-inflammatory effects of Aloe vera in mice with acetaminophen induced hepatitis. Male mice were randomly divided into three groups (n = 8 each). Control group were given orally distilled water (DW). APAP group were given orally N-acetyl-P-aminophenol (APAP) 400 mg/kg suspended in DW. Aloe vera-treated group were given orally APAP and Aloe vera (150 mg/kg) suspended in DW. Twenty-four hours later, the liver was removed to determine hepatic malondialdehyde (MDA), hepatic glutathione (GSH), the number of interleukin (IL)-12 and IL-18 positive stained cells (%) by immunohistochemistry method, and histopathological examination. Then, the serum was collected to determine transaminase (ALT). In APAP group, ALT, hepatic MDA and the number of IL-12 and IL-18 positive stained cells were significantly increased when compared to control group (1210.50 ± 533.86 vs 85.28 ± 28.27 U/L, 3.60 ± 1.50 vs 1.38 ± 0.15 nmol/mg protein, 12.18 ± 1.10 vs 1.84 ± 1.29%, and 13.26 ± 0.90 vs 2.54 ± 1.29%, P = 0.000, respectively), whereas hepatic GSH was significantly decreased when compared to control group (5.98 ± 0.30 vs 11.65 ± 0.43 nmol/mg protein, P = 0.000). The mean level of ALT, hepatic MDA, the number of IL-12 and IL-18 positive stained cells, and hepatic GSH in Aloe vera-treated group were improved as compared with APAP group (606.38 ± 495.45 vs 1210.50 ± 533.86 U/L, P = 0.024; 1.49 ± 0.64 vs 3.60 ± 1.50 nmol/mg protein, P = 0.001; 5.56 ± 1.25 vs 12.18 ± 1.10%, P = 0.000; 6.23 ± 0.94 vs 13.26 ± 0.90%, P = 0.000; and 10.02 ± 0.20 vs 5.98 ± 0.30 nmol/mg protein, P = 0.000, respectively). Moreover, in the APAP group, the liver showed extensive hemorrhagic hepatic necrosis at all zones while in Aloe vera-treated group, the liver architecture was improved histopathology. APAP overdose can cause liver injury. Our result indicate that Aloe vera attenuate APAP

Evaluation of antimicrobial efficacy of Aloe vera and its effectiveness in decontaminating gutta percha cones.

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Athiban, Prakash P; Borthakur, Bikash Jyoti; Ganesan, S; Swathika, B

2012-07-01

The aim of this study was to evaluate the antimicrobial efficacy of Aloe vera and to determine its effectiveness in decontaminating gutta percha cones. A concentrated extract of Aloe vera was used to check for the antimicrobial efficacy using the agar well diffusion method. Presence of zones' of diffusion was identified against three common GP contaminants namely, E.coli, E.faecalis and Staph. aureus. New GP Cones, freshly taken out of the packet were then decontaminated for 1minute using Aloe vera gel and then placed in thioglycolate broth to check for the presence of turbidity. The zones of inhibition on the agar plate were measured as 24mm,21mm and 24mm respectively. The broth remained clear even after 48 hours of incubation. We conclude that Aloe vera is indeed effective as a GP decontaminant and it holds a promising future as a medium for storage of GP cones.

¿El principio del fin o el fin del principio? - Apuntes para una revolución

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Dr. Ángel Luis Acosta Romero

1998-01-01

Full Text Available Al final del segundo milenio nos encontramos, sin duda, en una encrucijada histórica. Y no creemos que se trate sólo, aunque también, de un tópico repetido, y, casi siempre, adornado con tintes apocalípticos. Una encrucijada es un cruce de caminos, es decir, la duda y la apertura a distintas posibilidades. La duda, es cierto, nos obliga elegir. La libertad, en este caso, se nos impone. Debemos elegir y debemos hacerlo siendo conscientes de nuestros límites y de nuestras potencialidades. La crisis de la sociedad -de un modelo de sociedad- refleja y es reflejada por la crisis del conocimiento. Ya sabemos que el sujeto y el objeto se configuran mutuamente y no pueden ser aislados artificialmente. Afrontar la revolución en el conocimiento es afrontar, al mismo tiempo, una revolución política, es decir, una revolución integral, en la que el hombre (la humanidad se reconozca en su devenir complejo (homo sapiens/demens le llama Morin.

The efficacy of aloe vera in management of oral lichen planus: a systematic review and meta-analysis.

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Ali, S; Wahbi, W

2017-10-01

The main objective of oral lichen planus management is to control symptomatic outbreaks via eliminating lesions and alleviating symptoms. Corticosteroids, calcineurin inhibitors, and most of the available modalities are associated with adverse effects, which may limit their use. The aim of this review was to assess the effect of aloe vera on pain alleviation and/or clinical improvement in patients with symptomatic oral lichen planus. MEDLINE-PubMed; CENTRAL, and LILACS were searched in May 2016 to identify all clinical trials evaluating aloe vera vs placebo or corticosteroids for treatment of oral lichen planus. The included studies were five clinical trials and two case reports. From the included studies, there is weak evidence that aloe vera is more efficient than placebo and has comparable effect to triamcinolone acetonide. The meta-analyses show heterogeneity and reveals that aloe vera is inferior to the control in general. Although corticosteroids are still the gold standard, aloe vera shows promising results especially with no adverse effects compared with various adverse effects of corticosteroids. We recommend conducting new randomized clinical trials with larger sample size, sufficiently long follow-up period as well as standardized aloe vera formulations and outcome measures. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Mapping Sargassum beds off, ChonBuri Province, Thailand, using ALOS AVNI2 image

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Noiraksar, Thidarat; Komatsu, Teruhisa; Sawayama, Shuhei; Phauk, Sophany; Hayashizaki, Ken-ichi

2012-10-01

Sargassum species grow on rocks and dead corals and form dense seaweed beds. Sargassum beds play ecological roles such as CO2 uptake and O2 production through photosynthesis, spawning and nursery grounds of fish, feeding ground for sea urchins and abalones, and substrates for attached animals and plants on leaves and holdfasts. However, increasing human impacts and climate change decrease or degrade Sargassum beds in ASEAN countries. It is necessary to grasp present spatial distributions of this habitat. Thailand, especially its coastal zone along the Gulf of Thailand, is facing degradation of Sargassum beds due to increase in industries and population. JAXA launched non-commercial satellite, ALOS, providing multiband images with ultra-high spatial resolution optical sensors (10 m), AVNIR2. Unfortunately, ALOS has terminated its mission in April 2011. However, JAXA has archived ALOS AVNIR2 images over the world. They are still useful for mapping coastal ecosystems. We examined capability of remote sensing with ALOS AVNIR2 to map Sargassum beds in waters off Sattahip protected area as a natural park in Chon Buri Province, Thailand, threatened by degradation of water quality due to above-mentioned impacts. Ground truth data were obtained in February 2012 by using continual pictures taken by manta tow. Supervised classification could detect Sargassum beds off Sattahip at about 70% user accuracy. It is estimated that error is caused by mixel effect of bottom substrates in a pixel with 10 x 10 m. Our results indicate that ALOS AVNIR2 images are useful for mapping Sargassum beds in Southeast Asia.

Pharmacological attribute of Aloe vera: Revalidation through experimental and clinical studies

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Gupta, Vinay K.; Malhotra, Seema

2012-01-01

Aloe vera has long been used as a traditional medicine for inducing wound healing. It is a natural product that now a days is used in cosmetic industry. Benefits associated with Aloe vera have been attributed to the polysaccharides contained in the gel of the leaves though there are various indications for its use. Biological activities include promotion of wound healing, antifungal activity, anti-inflammatory, anticancer and immunomodulatory. Gingival fibroblasts play an important role in oral wound healing. Double blind-controlled trials are needed to determine its real efficacy in oral health. PMID:23559789

Identificación del genotipo del virus del papiloma humano en pacientes portadoras de lesiones cérvico uterinas

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Alexander Morales Fontaine

2016-02-01

Full Text Available Fundamento: en los últimos años se ha observado un elevado predominio de mujeres infestadas por genotipos del  Virus del Papiloma Humano considerados de alto riesgo por su elevada oncogenicidad.Objetivo: identificar los genotipos del Virus del Papiloma Humano en pacientes portadoras de este con lesiones cervicales, cuyas biopsias fueron analizadas en el departamento de anatomía patológica, perteneciente al Hospital General Docente ¨Ernesto Guevara de la Serna¨, en el período comprendido entre enero de 2013 a diciembre de 2014.Métodos: se realizó un estudio descriptivo, de corte transversal, en 45 pacientes cuyas biopsias fueron tomadas de forma aleatoria de un total de 456 casos en los que se encontraron evidencias histopatológicas de infección por el  Virus del Papiloma Humano, en el período referido período de estudio. Las biopsias fueron enviadas al Laboratorio de Biología Molecular del departamento de Virología del Instituto de Medicina Tropical ´´Pedro Kourí´, a fin de aislar e identificar los tipos de este virus, involucrados en la patogénesis de las lesiones premalignas en estudio. Se utilizaron fuentes secundarias dadas por los resultados de la identificación del genotipo del virus aislado enviados desde dicha institución. Los datos se procearon según la estadística descriptiva, mediante el análisis porcentual.Resultados: en el 88,8 % de la muestra se obtuvo infestación por el Virus del Papiloma Humano, de alto riesgo de oncogenicidad, de ellos hay una preponderancia del genotipo 16, con 26 casos (57,7 % seguido del 31 con 12 aislamientos (26,6 % y 2 pacientes presentaron el genotipo 52 para el 4,4 %. El 6,6 % de la muestra resultó estar infestadas por el genotipo 11, el cual es considerado de bajo riesgo y en 2 casos no fue posible realizar la tipificación.Conclusiones: Existió gran predominio de pacientes afectadas por Virus de Papiloma Humano de alto riesgo de oncogenicidad.

The effect of Aloe vera bioactive and anthraquinone on the performance of laying hens

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Tiurma Pasaribu

2005-10-01

Full Text Available An experiment was conducted to study the use of Aloe vera bioactives as feed additives on the performances of laying hens. The Aloe vera bioactives was prepared as the dry gel (DG and semi liquid gel (SLG. The Aloe vera was suplemented into the diets with concentration of equal to 0.5 and 1.0 g DG/kg diets. Diets contained commercial anthraquinone, a bioactive compound of Aloe vera with doses equal to 0.5 g DG and 1.0 dg/kg were also prepared. Diets were compared to control diets containing with and without antibiotic. Two hundred of fifty six laying hens strain Isa Brown aged 19 weeks were used for the experiment. Each treatment had 8 replicates with 4 hens in each replicate. The treatments were carried out for 30 weeks and parameters measured were egg production (% hen day/HD, egg weight, feed conversion ratio (FCR, feed consumption, egg quality, and mortality. Results showed that feed consumption was not significantly different (P>0.05, however DG 1.0 g/kg and anthraquinone 0.5 g/kg tended to decrease the feed consumption. Egg production was not significantly affected by antibiotic, DG, SLG, or anthraquinone (0.5 g/kg, but anthraquinone 1.0 g/kg had more egg production than control. Higher concentration of DG, SLG, and anthraquinone 1.0 g/kg gave better FCR than those of lower dosage (0.5 g/kg. Haugh unit was not affected by the treatment while yolk weight, egg shell and shell weight was significantly decreased by anthraquinone 0.5 g/kg (P<0.05. Mortality from all treatments was only 1.6%. It was concluded that treated by anthraquinone was better than that by Aloe vera, however, they were not significantly different. For the healthy reason, the use of Aloe vera is more saver than the use of anthraquinone.

Pemanfaatan Ekstrak Andrographis Paniculata Nees Dan Aloe Vera L Sebagai Anti Inflamas

OpenAIRE

Evacuasiany W., Endang; Soebiantoro, Freddy

2002-01-01

Telah dilakukan pengujian terhadap tanaman Andrographis paniculata Nees dan Aloe Vera L dengan metoda artritis eksperimental pada tikus putih galur Wistar. Hasil percobaan menunjukkan adanya efek inhibisi radang yang diinduksi oleh karagenin lambda. Ekstrak etanol Andrographis paniculata Nees bermakna (p=0.05) pada dosis 0.9 g/kg BB dan Aloe vera L memberikan efek inhibisi radang pada dosis 1.5 g/kg BB (p=0.05). Pengujian dilakukan pada dosis yang bervariasi. Ternyata efek yang ditimbulkan te...

Protective effect of gel form of gastric gavage applicated aloe vera on ischemia reperfusion injury in renal and lung tissue.

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Sahin, Hasan; Yener, Ali Umit; Karaboga, Ihsan; Sehitoglu, Muserref Hilal; Dogu, Tugba; Altinisik, Hatice Betul; Altinisik, Ugur; Simsek, Tuncer

2017-12-30

The aloe vera plant has become increasingly popular in recent years. This study aimed to research the effect of aloe vera to prevent renal and lung tissue damage in an experimental ischemia-reperfusion (I/R) injury model. The study included 21 male Wistar Albino rats, which were categorized into control group, n = 7 (no procedures), Sham group n = 7 (I/R); and aloe vera therapy group, n = 7 (aloe vera and I/R). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were evaluated from lung and kidney tissues for biochemical investigations. As histopathological, hematoxylin and eosin and anti-iNOS were also examined. In biochemical investigations, SOD, CAT, and GPx levels of the Sham group were found to be lower compared with the other groups (P < 0.05). The aloe vera therapy group was not statistically different from control groups but significantly different compared with the Sham group. In the same way, the MDA levels of kidney and lung tissues were statistically significant in the aloe vera therapy group, compared to the Sham group. In the Sham group, the peribronchial and perialveolar edema were observed in lung parenchyma. Also, excess interstitial hemorrhage, leukocyte infiltration, and alveolar wall thickening were identified in ischemic groups. The histopathological changes were much lighter than in the aloe vera therapy group. In renal tissues, excess epithelial cell deterioration, tubular desqumination, and glomerular atrophy were observed in the Sham group. The histopathological changes were markedly reduced in the aloe vera therapy  group. In the kidney and lung tissue, the level of iNOS activity in the Sham group was significantly higher than in the control and aloe vera therapy group. This study indicated that aloe vera is protective against oxidative damage formed by I/R in distant organs like the lungs and kidneys.

HISTORIA DEL ECMO (OXIGENACIÓN POR MEMBRANA EXTRACORPÓREA O SOPORTE VITAL EXTRACORPÓREO)

OpenAIRE

Rodrigo Díaz, Dr.; Christian Fajardo, EU.; Jorge Rufs, Dr.

2017-01-01

RESUMEN: EL ECMO u oxigenación por membrana extracorpórea, tiene una larga historia de desarrollo. Partiendo con el estudio del aparato circulatorio, transfusiones sanguíneas y circulación extracorpórea en el quirófano. Hay casos aislados reportados en la segunda mitad de la década de 1960, el primer paciente vivo se publicó en el NEJM en 1972. Existe suficiente evidencia para pensar que tiene un impacto positivo en la falla respiratoria neonatal y en la misma condición en adultos, si estos s...

Development of Aloe vera based edible coating for tomato

Science.gov (United States)

Athmaselvi, K. A.; Sumitha, P.; Revathy, B.

2013-12-01

The effect of formulated Aloe vera based edible coating on mass loss, colour, firmness, pH, acidity, total soluble solid, ascorbic acid and lycopene on the coated tomato was investigated. The tomato in control showed a rapid deterioration with an estimated shelf life period of 19 days, based on the mass loss, colour changes, accelerated softening and ripening. On the contrary, the coating on tomatoes delayed the ripening and extended the shelf life up to 39 days. The physiological loss in weight was 7.6 and 15.1%, firmness was 36 and 46.2 N on 20th day for control and coated tomatoes, respectively. From the results, it was concluded that the use of Aloe vera based edible coating leads to increased tomato shelf-life.

ALOE VERA BARBADENSIS MILLER AS AN ALTERNATIVE TREATMENT FOR CHILDREN WITH FEVER

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Siti Choirul Dwi Astuti

2017-10-01

Full Text Available Background: Applying a cooling treatment from outside the body is one way to lower fever in children. Midwives commonly perform a warm water compress in their care, but it is considered less effective. Thus, compress with aloe vera barbadensus miller is proposed in this study as an alternative treatment. Objective: To examine the effect of Aloe vera barbadensis miller compress in reducing body temperature in children with fever. Methods: This was a quasi-experimental study with pretest and posttest with control group design. Forty children were recruited, with twenty of them assigned in each group. Purposive sampling was performed to select the sample. A digital rectal thermometer was used to measure the temperature. Independent t- test and paired test were used for data analysis. Results: Findings showed that the aloe vera compress group showed a higher decrease of body temperature compared to the warm water compress group. The difference of body temperature after 20 minutes in the experiment group was 1.435 while in the control group was only 1.085. There were statistically significant differences in body temperature between the experiment and control group (p=0.013. Conclusion: Aloe vera barbadensis miller compress is an effective alternative therapy in reducing body temperature in children with fever. It is recommended for midwives to apply this intervention to reduce body temperature significantly.

Aloe vera gel and cesarean wound healing; a randomized controlled clinical trial.

Science.gov (United States)

Molazem, Zahra; Mohseni, Fatemeh; Younesi, Masoumeh; Keshavarzi, Sareh

2014-08-31

Failure in complete healing of the wound is one of the probable complications of cesarean. The present study aimed to determine the effectiveness of dressing with aloe vera gel in healing of cesarean wound. This prospective randomized double-blind clinical trial was conducted on 90 women who had undergone cesarean operation in Amir-al-Momenin hospital (Gerash, Iran). The participants were randomly divided into two groups each containing 45 patients. In one group, the wound was dressed with aloe vera gel, while simple dressing was used in the control group. Wound healing was assessed 24 hours and 8 days after the cesarean operation using REEDA scale. The data were analyzed through Chi-square and t-test. The participants' mean age was 27.56±4.20 in the aloe vera group and 26.62±4.88 in the control group, but the difference was not statistically significant. However, a significant difference was found between the two groups concerning body mass index, heart rate, and systolic blood pressure (Paloe vera group and 35 ones in the control group had obtained a zero score 24 hours after the operation. These measures were respectively obtained as 42 and 41eight days after the operation. According to the findings of this study, the women are recommended to be informed regarding the positive effects of dressing with aloe vera gel.

Development Prospect of Aloe Vera Plant in Peatlands in West Kalimantan

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Tommy Purba

2016-04-01

Full Text Available Peat soil is one of the decent growing medium for aloe vera plant. Seeing the potential of peat land in West Kalimantan is quite extensive, this plant is cultivated commercially and becomes one of the leading commodity in peatlands in West Kalimantan, especially in Pontianak City with an area of more than 25,000 ha. With complete and varied nutrient content, this plant progress opportunities, especially for diversification is enormous ranging from food products and beverages such as juices, cocktails, aloe vera gel in syrup, jams, jellies, tarts, sweets, tea, chips and as well as agro-tourism in West Kalimantan. Existing obstacles now are market share and unstable price, trouble controlling plant diseases and peat area is flooded during the rainy season. Prospects for the future development of these plants can be carried out through the cooperation of government and relevant stakeholders,stable markets and prices, the acceleration of product promotion through fairs and business gathering activities, as well as the dissemination and application of cultivation technology and post-harvest of aloe vera.

Millimeter Detection of AlO (X^2 Σ ^+): Metal Oxide Chemistry in the Envelope of VY Canis Majoris

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Tenenbaum, E. D.; Ziurys, L. M.

2009-06-01

A new circumstellar molecule, the radical AlO (X^2 Σ ^+), has been detected toward the envelope of the oxygen-rich supergiant star VY Canis Majoris (VY CMa) using the Arizona Radio Observatory (ARO). The N = 7 → 6 and 6 → 5 rotational transitions of AlO at 268 and 230 GHz were observed at 1 mm using the ARO Submillimeter Telescope (SMT) and the N = 4 → 3 line was detected at 2 mm using the ARO 12 m. Based on the shape of the line profiles, AlO most likely arises from the dust-forming region in the spherical outflow of VY CMa, as opposed to the blue- or red-shifted winds, with a source size of θ_s ˜ 0.5^''. Given this source size, the column density of AlO was found to be N_{tot} ˜ 2 × 10^{15} cm^{-2} for T_{rot} ˜ 230 K, with a fractional abundance, relative to H_2, of ˜ 10^{-8}. Gas-phase thermodynamic equilibrium chemistry is the likely formation mechanism for AlO in VY CMa, but shocks may disrupt the condensation process into Al_2O_3, allowing AlO to survive to a radius of ˜ 20 R_*. The detection of AlO in VY CMa is additional evidence of an active gas-phase refractory chemistry in oxygen-rich envelopes, and suggests such objects may be fruitful sources for other new oxide identifications.

The Effect of Aloe, Gelfoam, Plaster on Bone Formation in applying to the bone defect

International Nuclear Information System (INIS)

Choi, Eui Hwan; Kim, Su Gwan

1999-01-01

This study was to evaluate the effects of Aloe, Gelfoam, and Plaster of Paris on bone healing. Four experimental defects were created for placement of the three materials in the right femur of dogs. One defect served as an empty control site. The evaluation was performed at 1-, 6-, and 12-weeks by light microscopy and NIH image program. Radiographic and Histologic examinations showed new bone formation in the presence of Aloe, Gelfoam, and Plaster of Paris and similar bone healing reactions. On the basis of these findings, it was concluded that Aloe, Gelfoam, and Plaster of Paris may be adequate agents for use in bone procurement.

Effect Of Aloe Vera Juice On Hyperglycemia And ATHEROGENICITY In Diabetic Rats

International Nuclear Information System (INIS)

ABDEL-AZIZ, A.F.; EZZ-ELARAB, A.; EL-SHERBINY, E.M.; MORSI, R.M.

2009-01-01

Diabetes mellitus is the most prevalent chronic disease and cause death in many countries. The present study aims to study the efficacy of Aloe vera whole leaf juice filtrate to ameliorate the glucose level and lipid profile status in four groups of female diabetic rats. Serum glucose, total cholesterol, HDL-cholesterol, anti-atherogenic index (AAI), TBARs and insulin levels were determined in all groups. There was very highly significant increase in serum glucose, cholesterol, and TBARs levels in diabetic group as compared to the control. Oral administration of Aloe vera juice filtrate resulted in a very highly significant decrease in serum glucose, cholesterol and TBARs levels when compared to that of diabetic group. Serum HDL-cholesterol, insulin level and anti-atherogenic index were very highly significantly decreased in diabetic rats as compared to the control, whereas these parameters were highly significantly increased after the oral administration of Aloe vera juice filtrate as compared to diabetic group

Efficacy of Aloe Vera Cream in the Treatment of Paederus Dermatitis in Mice

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Ramin Khaghani

2017-06-01

Full Text Available Background: Dermatitis caused by Paederus beetle involves many people around the world, especially Iran. The symptoms include redness, itching and severe irritation. This study evaluated the effectiveness of the Aloe vera cream on the treatment of dermatitis caused by Paederus beetles.Methods: Forty male 6–8 weeks BALB/C mice were randomly divided into four groups of 10 mice. After removing the mice’s back hair, the backs of mice were marked by a circle with a diameter of 3 mm. The Paederus beetles were collected from Babol in Mazandaran Province, northern Iran and transferred to the animal lab of Aja University of Medical Sciences, Tehran, Iran. The end of abdominal segment Paederus was cut with scissors and hemolymph content was pushed by forceps on the circle. Only hemolymph of one Paederus applied to the back of each mouse. Groups 1, 2, 3 and 4 were treated with the base (vehicle, dexamethasone 0.1%, Aloe vera 0.5% and Aloe vera 2% creams respectively. After 2 days, dermatitis appeared. Then the mentioned creams were applied on the mice once a day. The wound area was measured every day. Dermatitis surface area under curve (AUC of each mouse was cal­culated for 17 days after induction of dermatitis. Statistical analysis of ANOVA was used.Results: Application of Aloe vera 0.5% and 2% significantly reduced the healing duration and dermatitis area in comparison with the vehicle and dexamethasone cream (P< 0.05. But dexamethasone had no significant effect on the healing of dermatitis as compared to vehicle.Conclusion: Aloe vera may clinically effective in the treatment of Paederus dermatitis.

Economic Sustainability of Organic Aloe Vera Farming in Greece under Risk and Uncertainty

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Angelos Liontakis

2016-04-01

Full Text Available During the last decade, an encouraging environment for the restructuring and modernization of the agricultural sector has formed in Greece. The diversification into higher-value crops can be a promising option for small and average-sized farms, particularly during the current economic crisis. One of the most promising alternative crops that have been recently established in Greece is the organic Aloe vera crop. The main advantage of this crop is that it can utilize poor farmlands and, therefore, can facilitate rural development in marginal areas. This study explores the economic sustainability of the Aloe vera crop, considering the embedded risk and uncertainty. The results indicate that organic aloe farming is a promising alternative to “traditional” crops in Greece, particularly for family farms in rural areas. In contrast, this activity is not advisable to the most entrepreneurial type of farmers, unless their crop size allows economies of scales. Finally, the Stochastic Efficiency with Respect to a Function (SERF analysis associates farmers’ risk attitude with their willingness to be involved in organic Aloe vera farming. SERF analysis highlights the crucial role of farmers’ risk aversion and concludes that, above a certain level of risk aversion, farmers have no incentive to adopt this economic activity.

RBF neural network prediction on weak electrical signals in Aloe vera var. chinensis

Science.gov (United States)

Wang, Lanzhou; Zhao, Jiayin; Wang, Miao

2008-10-01

A Gaussian radial base function (RBF) neural network forecast on signals in the Aloe vera var. chinensis by the wavelet soft-threshold denoised as the time series and using the delayed input window chosen at 50, is set up to forecast backward. There was the maximum amplitude at 310.45μV, minimum -75.15μV, average value -2.69μV and Aloe vera var. chinensis respectively. The electrical signal in Aloe vera var. chinensis is a sort of weak, unstable and low frequency signals. A result showed that it is feasible to forecast plant electrical signals for the timing by the RBF. The forecast data can be used as the preferences for the intelligent autocontrol system based on the adaptive characteristic of plants to achieve the energy saving on the agricultural production in the plastic lookum or greenhouse.

Degradación de tiocianato por hongos aislados de ambientes mineros y evaluación de su capacidad degradativa

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Susan Medina

2012-10-01

Full Text Available El Perú es uno de los principales países en la extracción de minerales como el oro, zinc, plomo y estaño. En la producción de oro se utiliza cianuro, el cual es tóxico para el medio ambiente, y que por reacción natural con el azufre se convierte en tiocianato (-SCN. En el presente trabajo se aislan hongos con capacidad de degradar tiocianato procedentes de aguas y suelos de zonas mineras de Junín y Tumbes. Estas cepas se sometieron a ensayos con concentraciones de tiocianato entre 5 y 600 mM. Asimismo, se evaluó la cinética de degradación de tiocianato en medio Kwon partiendo de 1,2 g.L-1 de KSCN y luego se realizó la identificación morfológica. De un total de 58 mohos aislados, obtuvimos 4 mohos con excelentes capacidades de degradar tiocianato y que pueden ser utilizados en biorremediación. La capacidad degradativa de estas cepas fue en promedio 10,05 mg.L-1 de -SCN con una velocidad de 28,77 mg.L-1.h-1. Dos cepas fueron identificadas fenotípica y molecularmente como Fusarium trincictum usando el marcador ITS del gen rDNA.

Efecto de la temperatura sobre películas de un aislado proteico del suero bovino (WPI adsorbidas sobre la interfase aceite-agua

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Rodríguez Patino, Juan M.

2002-09-01

Full Text Available Heat-induced interfacial aggregation of a whey protein isolate (WPI with a high content of β-lactoglobulin, previously adsorbed at the oil-water interface, was studied by interfacial dynamic characteristics (interfacial tension and surface dilational properties performed in a automatic drop tensiometer coupled with microscopic observation and image analysis of the drop after heat-treatment. The temperature, ranging between 20 and 80 ºC, and protein concentration in aqueous bulk phase, ranging between 1.10-1 and 1.10-5% wt/wt, were studied as variables. The pH, and ionic strength were maintained constant at 5 and 0.05 M, respectively. During the heat-treatment, WPI films behave typically as viscoelastic with non-zero phase angle, but with increasing elastic characteristics as the heat-treatment progresses. During isothermal treatment the surface dilational modulus, E, increases and the interfacial tension, σ, and phase angle, φ, decrease with time to a plateau value. The time dependence of E can be quantified by a first-order equation according to two kinetic mechanisms. The rate of thermal changes in WPI adsorbed films increases with protein concentration in solution. Heat-treatment produces irreversible changes in WPI adsorbed films because the interfacial characteristics do not return to original values after cooling back to the initial temperature. Significant changes in interfacial characteristics and drop image associated with interfacial WPI gelation were observed at protein concentration as low as 1.10-5% wt/wt, even for heat-treatment at 40 ºC.En este trabajo se ha estudiado la agregación interfacial inducida por el calor en un aislado proteico del suero bovino (WPI, previamente adsorbido sobre la interfase aceite-agua. Se ha seguido la evolución, durante el tratamiento térmico, de las características dinámicas interfaciales (tensión interfacial y propiedades dilatacionales superficiales, determinadas en un tensi

Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

Science.gov (United States)

Winters, Wallace D.; Scott, Michael; David, Andrew; Gillis, Glenn; Stoufflet, Thaya; Nair, Anand; Kousoulas, Konstantine

2013-01-01

Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL) aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks. PMID:23554812

Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

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Inder Sehgal

2013-01-01

Full Text Available Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

Evaluation of efficacy of aloe vera in the treatment of oral submucous fibrosis - a clinical study.

Science.gov (United States)

Anuradha, Ardra; Patil, Bharati; Asha, Venkataswamy Reddy

2017-01-01

Oral submucous fibrosis is a chronic disease, treatment of which has largely been symptomatic. Aloe vera has immunomodulatory, anti-inflammatory, wound healing, antioxidant, and antineoplastic activities. All such properties of aloe vera suggest the possibility of its use in the management of oral submucous fibrosis. Seventy-four patients of oral submucous fibrosis were randomly divided into 2 groups. Group A patients were treated with systemic (juice) and topical aloe vera (gel) for 3 months. Group B patients were treated with intralesional injection of hydrocortisone and hyaluronidase for 6 weeks with antioxidant supplements for 3 months. Patients were assessed for reduction in burning sensation and increase in mouth opening, cheek flexibility, and tongue protrusion at an interval of 1, 2, and 3 months. Both the groups showed statistically significant improvements in all the study parameters at the end of study period (P aloe vera was comparable to that of intralesional injections of hydrocortisone and hyaluronidase with antioxidant supplementation. The study concludes that aloe vera can be an alternative, safe, and effective treatment regime in the management of oral submucous fibrosis. Long-term follow-up studies with larger sample size are recommended. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Anti-Atherogenicity Following Administration of Exudate from Aloe ...

African Journals Online (AJOL)

Anti-Atherogenicity Following Administration of Exudate from Aloe barbadensis Leaves in Diabetic Rats. HU Nwanjo, GO Oze. Abstract. No Abstract. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · AJOL African Journals Online. HOW TO USE AJOL... for Researchers ...

Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

African Journals Online (AJOL)

Extraction of the yellow colour compounds of leaves of Aloe excelsa were performed and 1,8-dihydroxy-3-methylanthracenedione (chrysophanol) was isolated and tested for antibacterial activities against four gram negative and five gram positive bacterial strains. The structures of chrysophanol was determined by chemical ...

Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages.

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Budai, Marietta M; Varga, Aliz; Milesz, Sándor; Tőzsér, József; Benkő, Szilvia

2013-12-01

Aloe vera has been used in traditional herbal medicine as an immunomodulatory agent inducing anti-inflammatory effects. However, its role on the IL-1β inflammatory cytokine production has not been studied. IL-1β production is strictly regulated both at transcriptional and posttranslational levels through the activity of Nlrp3 inflammasome. In this study we aimed to determine the effect of Aloe vera on the molecular mechanisms of Nlrp3 inflammasome-mediated IL-1β production in LPS-activated human THP-1 cells and monocyte-derived macrophages. Our results show that Aloe vera significantly reduced IL-8, TNFα, IL-6 and IL-1β cytokine production in a dose dependent manner. The inhibitory effect was substantially more pronounced in the primary cells. We found that Aloe vera inhibited the expression of pro-IL-1β, Nlrp3, caspase-1 as well as that of the P2X7 receptor in the LPS-induced primary macrophages. Furthermore, LPS-induced activation of signaling pathways like NF-κB, p38, JNK and ERK were inhibited by Aloe vera in these cells. Altogether, we show for the first time that Aloe vera-mediated strong reduction of IL-1β appears to be the consequence of the reduced expression of both pro-IL-1β as well as Nlrp3 inflammasome components via suppressing specific signal transduction pathways. Furthermore, we show that the expression of the ATP sensor P2X7 receptor is also downregulated by Aloe vera that could also contribute to the attenuated IL-1β cytokine secretion. These results may provide a new therapeutic approach to regulate inflammasome-mediated responses. Copyright © 2013 Elsevier Ltd. All rights reserved.

Dye-sensitized solar cells using Aloe Vera and Cladode of Cactus extracts as natural sensitizers

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Ganta, D.; Jara, J.; Villanueva, R.

2017-07-01

The purpose of this study is to develop dye-sensitized solar cells (DSSCs) from natural plant-based dyes, extracted from the Cladode (nopal) of the Thornless Prickly Pear Cactus (Opuntia ficus-indica), the gel of Aloe Vera (Aloe barbadensis miller), and the combination of Cladode and Aloe Vera extracts on side-by-side configuration. Optical properties were analyzed using UV-Vis Absorption and Fourier Transform Infrared Spectroscopy. Open circuit voltages (Voc) varied from 0.440 to 0.676 V, fill factors (FF) were greater than 40%, short-circuit photocurrent densities (Jsc) ranged from 0.112 to 0.290 mA/cm2 and highest conversion efficiency of 0.740% was reported for the Cladode DSSC.

AISLAMIENTO Y ELUCIDACIÓN ESTRUCTURAL DE ALGUNOS METABOLITOS MAYORITARIOS DEL EXTRACTO ETANÓLICO DE Solanum cornifolium. SECCIÓN GEMINATA

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Luis Enrique Cuca

2008-02-01

Full Text Available Del extracto etanólico de la parte aérea de Solanum cornifolium, utilizando técnicas cromatográficas para la separación y purificaciónde cada uno de los compuestos, fueron aislados dos esteroles, B-sitosterol y Estigmasterol, un triterpeno pentacíclico,Lupeol, y un nuevo alcaloide esteroidal 2-(-metil-tetrahirofurano-Solanidina.Sus estructuras fueron elucidadas por RMN, incluyendo técnicas 2D y por comparación con datos de la literatura.

Penapisan PGPF untuk Pengendalian Penyakit Busuk Lunak Lidah Buaya (Aloe vera di Tanah Gambut

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Supriyanto Supriyanto

2009-12-01

Full Text Available Aloe (Aloe vera planted in West Borneo peat soil is well known as having the best product quality in Indonesia. Soft rot disease is one of the constraints of Aloe cultivation on peat soil. Many methods have no significant result for controlling this disease. This research objectives are to obtain Plant Growth Promoting Fungi (PGPF for controlling Aloe bacterial soft rot in peat soil. The research was conducted in Clinical Laboratory of Plant Pathology and glass house of Faculty of Agriculture, Gadjah Mada University, Yogyakarta from October 2008 to September 2009. The methods included fungal isolation from peat land, hypovirulent and PGPF ability test, and biological control test in the glass house. Among 42 peat soil fungi tested, 28 isolates were hypovirulent and only 2 isolates i.e. SNTH001 (Penicillium sp. and SNTH003 (Aspergillus sp. showed the PGPF ability. Glass house trial showed that single application of SNTH001 and SNTH003 isolates were able to increase the growth of Aloe. In the biological control of Aloe soft rot disease test showed that the lowest intensity (25% might be obtained by using SNTH001 isolate.   Lidah buaya (Aloe vera asal tanah gambut Kalimantan Barat dikenal mempunyai kualitas terbaik di Indonesia. Penyakit busuk lunak yang disebabkan oleh bakterimerupakan salah satu kendala dalam pengembangan tanaman lidah buaya dilahan gambut dan beberapa cara pengendalian yang telah dilakukan belum memberikan hasil nyata. Penelitian ini bertujuan untuk mencari jamur asal tanah gambut yang mampu berperan sebagai Pemacu Pertumbuhan Tanaman untuk pengendalian penyakit busuk lunak di tanah gambut. Penelitian telah dilakukan di Laboratorium Ilmu Penyakit Tumbuhan Klinik dan Rumah Kaca Fakultas Pertanian UGM Yogyakarta dari bulan Oktober 2008 sampai September 2009, meliputi isolasi jamur dari tanah gambut, uji hipovirulensi dan uji kemampuan sebagai PGPF serta uji pengendalian hayati penyakit busuk lunak lidah buaya di rumah kaca. Dari

Gamma irradiation response of RBC in presence of Aloe vera leaf extract

International Nuclear Information System (INIS)

Rane, Charusheela C.; Patil, Shilpa M.; Kulkarni, Satish G.

2004-01-01

Full text: The components from fleshy leaves of Aloe vera have wide range of utility in treating human aliments owing to their medicinal property including antioxidant characteristic. During gamma irradiation, attack by free radicals is the prominent cause of damage to cell membrane. This action is modified to varied extent by several components from plants. A radio modulation effect of different dilutions of Aloe vera leave extract against gamma radiation has been evaluated by measuring percent haemolysis. The result obtained will be discussed to narrate influencing role of various factors in modulation

Aloe emodin, an anthroquinone from Aloe vera acts as an anti aggregatory agent to the thermally aggregated hemoglobin

Science.gov (United States)

Furkan, Mohammad; Alam, Md Tauqir; Rizvi, Asim; Khan, Kashan; Ali, Abad; Shamsuzzaman; Naeem, Aabgeena

2017-05-01

Aggregation of proteins is a physiological process which contributes to the pathophysiology of several maladies including diabetes mellitus, Huntington's and Alzheimer's disease. In this study we have reported that aloe emodin (AE), an anthroquinone, which is one of the active components of the Aloe vera plant, acts as an inhibitor of hemoglobin (Hb) aggregation. Hb was thermally aggregated at 60 °C for four days as evident by increased thioflavin T and ANS fluorescence, shifted congo red absorbance, appearance of β sheet structure, increase in turbidity and presence of oligomeric aggregates. Increasing concentration of AE partially reverses the aggregation of the model heme protein (hemoglobin). The maximum effect of AE was observed at 100 μM followed by saturation at 125 μM. The results were confirmed by UV-visible spectrometry, intrinsic fluorescence, ThT, ANS, congo red assay as well as transmission electron microscopy (TEM). These results were also supported by fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD) which shows the disappearance of β sheet structure and appearance of α helices. This study will serve as baseline for translatory research and the development of AE based therapeutics for diseases attributed to protein aggregation.

In vivo diabetic wound healing effect and HPLC–DAD–ESI–MS/MS profiling of the methanol extracts of eight Aloe species

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Abeer M. El Sayed

Full Text Available Abstract Genus Aloe, Xanthorrhoeaceae, is well distributed all over Egypt, and many species have been used as medicinal plants; mainly reported to prevent cardiovascular diseases, cancer and diabetes. This study attempts to analyze the secondary metabolites in the methanol extract of the leaves of eight Aloe species; A. vera (L. Burm. f., A. arborescens Mill., A. eru A. Berger, A. grandidentata Salm-Dyck, A. perfoliata L., A. brevifolia Mill., A. saponaria Haw. and A. ferox Mill. growing in Egypt. For this aim HPLC–DAD–MS/MS in negative ion mode was used. Although belonging to the same genus, the composition of each species presented different particularities. Seventy one compounds were identified in the investigated Aloe species, of which cis-p-coumaric acid derivaties, 3,4-O-(E caffeoylferuloylquinic acid and caffeoyl quinic acid hexoside were the most common phenolic acids identified. Aloeresin E and isoaloeresin D, 2'-O-feruloylaloesin were the common anthraquinones identified. Lucenin II, vicenin II, and orientin were the common identified flavonoids in the investigated Aloe species. 6'-Malonylnataloin, aloe-emodin-8-O-glucoside, flavone-6,8-di-C-glucosides could be considered as chemotaxonomic markers for the investigated Aloe species. The eight Aloe species had significant anti-inflammatory activity, in addition to the significant acceleration of diabetic wound healing in rats following topical application of the methanol extracts of their leaves. This is the first simultaneous characterization and qualitative determination of multiple phenolic compounds in Aloe species from locally grown cultivars in Egypt using HPLC–DAD–MS/MS, which can be applied to standardize the quality of different Aloe species and the future design of nutraceuticals and cosmetic preparations.

The increasing of fibroblast growth factor 2, osteocalcin, and osteoblast due to the induction of the combination of Aloe vera and 2% xenograft concelous bovine

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Utari Kresnoadi

2012-12-01

Full Text Available Background: To make a successfull denture prominent ridge is needed, preservation on tooth extraction socket is needed in order to prevent alveol bone resorption caused by revocation trauma. An innovative modification of the material empirically suspected to be able reduce inflammation caused by the revocation trauma is a combination of Aloe vera and xenograft concelous bovine (XCB and Aloe vera is a biogenic stimulator and accelerating the growth of alveolar ridge bone after tooth extraction. Purpose: The research was aimed to determine of the increasing alveol bone formation by inducing the combination of Aloe vera and 2% xenograft concelous bovine. Methods: To address the problems, the combination of Aloe vera and xenograft concelous bovine was induced into the tooth extraction sockets of Cavia cabayas which devided on 8 groups. Groups control, filled with XCB, Aloe vera and Aloe vera and XCB combination, at 7 days and 30 days after extraction. Afterwards, immunohistochemical examination was conducted to examine the expressions of FGF-2 and osteocalcin, as the product of the growth of osteoblasts. Results: There were significantly increases expression of FGF-2 and osteocalcyn on group which filled with XCB, Aloe vera and combined Aloe vera and XCB. Conclusion: It may be concluded that the induction of the combination of Aloe vera and xenograft concelous bovine into the tooth sockets can enhance the growth expressions of FGF-2 and osteocalcin as the product of osteoblasts, thus, the growth of alveolar bone was increased.Latar belakang: Untuk keberhasilan pembuatan gigitiruan diperlukan ridge yang prominent, maka diperlukan suatu preservasi soket pencabutan gigi untuk mencegah terjadinya resopsi tulang alveolar akibat trauma pencabutan. Suatu inovasi modifikasi bahan yang diduga secara empiris dapat mengurangi keradangan karena trauma pencabutan adalah berupa kombinasi Aloe vera dan xenograft concelous bovine (XCB. Aloe vera yang merupakan

The effects of crude aqueous and alcohol extracts of Aloe vera on growth and abdominal viscera of suckling rats.

Science.gov (United States)

Beya, Wabeya; Davidson, Bruce; Erlwanger, Kennedy H

2012-01-01

The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day old Sprague-Dawley rats were gavaged with aqueous or alcohol extracts of Aloe vera (low dose 50mg. kg⁻¹ or high dose 500mg. kg⁻¹) daily for eight days. All data were expressed as mean ± SD and analyzed by one way ANOVA. Pups receiving high doses of either extract had a significantly higher body mass gain than the group receiving lower dose (p Aloe vera extracts resulted in growth promotion, enhanced hepatic storage of metabolic substrates, increased ALP possibly in relation to bone growth and caused hypertrophy of the caecum of neonatal rats. These effects need to be explored further to enhance animal production and health.

ESTUDIO MORFOLÓGICO DEL CULTIVO A LARGO PLAZO DE FOLÍCULOS AISLADOS Y CERRADOS DE TIROIDES DE CERDO

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M HERRERA

2008-09-01

Full Text Available La unidad morfológica y funcional de la glándula tiroides es el folículo, estructura ovoide cerrada, constituida por una capa de células cúbicas (tirocitos que encierran un lumen lleno del coloide secretado por ellas. En cultivo, tanto la estructura como la función del folículo se pierden rápidamente en las primeras 24 horas. Sin embargo, si se cultivan folículos cerrados de tiroides de rata conservan la arquitectura folicular, la morfología del tirocito y la función hasta la síntesis de hormonas tiroides de manera similar a la glándula in vivo. En este trabajo describimos el aislamiento y cultivo de folículos porcinos cerrados y su análisis morfológico. Los folículos se aíslan por digestión enzimática y disociación mecánica del parénquima tiroideo, luego se cultivan sobre agarosa con y sin hormona tirotrópica o tirotropina (1 mU/ml, TSH. El tejido de tiroides porcino obtenido tiene las mismas características de una glándula hipotiroidea in vivo, un epitelio casi plano, retículo endoplásmico rugoso (RER exiguo, complejo de Golgi (CG, y microvellosidades escasas y cortas. Los folículos cultivados sin TSH conservan la forma ovoide y el coloide en su interior, y la misma ultra-estructura del tejido in vivo, RER y CG muy escasos, pero con el tiempo de cultivo aumenta la longitud de la microvellosidades y el espesor del epitelio. En presencia de TSH el epitelio se hipertrofia desde el primer día y las cavidades foliculares se reducen considerablemente. Se demuestra que folículos cerrados de tiroides durante ocho días (d de cultivo conservan su morfología con y sin TSH. Además, estos responden al estímulo de TSH disminuyendo su cavidad folicular y aumentando el espesor del epitelio folicular.

Inkassofirma ähvardab Alo Streimanni kaatrifirmat kohtuga / Andres Kärssin

Index Scriptorium Estoniae

Kärssin, Andres, 1971-

2005-01-01

Eesti Posti eksjuhile Alo Streimannile kuuluv kaatritehas ScandiNaval ja temaga seotud ehitusfirma OÜ TRK Ehitus on võlgades. Vt. samas: Gea Velthut-Sokka. Võlad on ka Streimanniga seotud ehitusfirmal

Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress

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Fábio Sabbadin Zanuzzo

2012-10-01

Full Text Available This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus, to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS only at 0.1, 0.2, 0.5 and 1 mg/L. Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested.

Effects of Six Weeks Endurance Training and Aloe Vera Supplementation on COX-2 and VEGF Levels in Mice with Breast Cancer

Science.gov (United States)

Shirali, Saeed; Barari, Alireza; Hosseini, Seyed Ahmad; Khodadi, Elaheh

2017-01-01

The aim of this study was to determine effects of six weeks endurance training and Aloe Vera supplementation on COX-2 and VEGF levels in mice with breast cancer. For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Aloe Vera and Aloe Vera + training. Breast cancer tumors were generated in mice by implantind. The training program comprised six weeks of swimming training accomplished in three sessions per week. Training time started with 10 minutes on the first day and increased to 60 minutes in the second week and the water flow rate was increased from 7 to 15 liters per minute at a constant rate. Aloe Vera extract at a dose of 300 mg/kg BW was administrated to rats by intraperitoneal injection. At the end of the study period, rats were anesthetized and blood samples were taken. Significant differences were concluded at pAloe Vera extract caused significant decrease in the COX-2 level in the cancer group. Also, in the training (swimming exercise) and Aloe Vera + training cancer groups, we observed significant decrease in the VEGF level as compared to controls. Our results suggest that Aloe Vera and training inhibit the COX pathway and cause decrease production of prostaglandin E2. Hence administration of Aloe Vera in combination with endurance training might synergistically improve the host milieu in mice bearing breast cancers. Creative Commons Attribution License

Influence of Polysaccharides from Aloe vera (Aloe barbadensis Miller, Liliaceae on Mechanically Induced Cough in Cats

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Martina Šutovská

2010-01-01

Full Text Available Different plants have been reported to be a source of polysaccharides that possess a great variety of biological activities, including cough suppression. The experiments were aimed on the influence of two polysaccharides isolated from the gel (A, HF1-Z and one from the epidermis (SL-1 of Aloe vera leaves, characterized by chemical composition and structural properties, on the cough reflex. The method of mechanically induced cough was used in conscious cats. A nylon fibre directed individually into the mucous area of the laryngopharyngeal and tracheobronchial airways via endotracheal cannula elicited the cough reflex. Statistical evaluation registered cough reflex indicators (number of cough efforts - NE, cough frequency - NE min–1 intensity of cough attacks during expiration and inspiration - IA+ and IA-, intensity of maximal inspiratory and expiratory cough efforts - IME- and IME+ showed that gel polysaccharide HF1-Z possessed higher cough-suppressing activity than dropropizine, a nonnarcotic peripheral cough suppressant. Their antitussive activity was accompanied mainly with a decrease of NE, IA+ and IA- from both laryngopharyngeal and tracheobronchial mucous areas of the airways. Results of the presented study also showed minimal influence of polysaccharide HF1-Z on expectoration predictor, IME- and IME+. New pharmacodynamic property of polysaccharides isolated from Aloe vera was shown during experiments, which could be a base of their possible therapeutic use in this indication in future.

Karyotypes in Ethiopian Aloe species (Xanthorrhoeaceae: Asphodeloideae)

DEFF Research Database (Denmark)

Fentaw, Eshetu; Dagne, Kifle; Rønsted, Nina

2013-01-01

A cytogenetic survey of 18 species in the succulent-leaved genus Aloe L. (Xanthorrhoeaceae subfamily Asphodeloideae) in Ethiopia was undertaken towards a more complete genetic characterisation of the genus throughout its distribution in Africa, Arabia and Madagascar. Somatic metaphase chromosomes...

CARACTERIZACIÓN DE BACTERIAS PROBIÓTICAS AISLADAS DEL INTESTINO GRUESO DE CERDOS COMO ALTERNATIVA AL USO DE ANTIBIÓTICOS

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Henry Jurado G

2009-08-01

Full Text Available Objetivos. Caracterizar bacterias ácido lácticas probióticas aisladas del intestino grueso de cerdos adultos como posible alternativa al uso de antibióticos. Materiales y métodos. Se incluyeron muestras de intestino grueso de 20 cerdos adultos, para el aislamiento de bacterias lácticas (BAL. La caracterización de los parámetros bioquímicos-fisiológicos de las BAL contemplo la evaluación del pH del fermento, determinación de ácidos orgánicos por HPLC y detección de isómeros de ácido láctico. Los procedimientos microbiológicos evaluaron la capacidad de inhibición del crecimiento de patógenos y la actividad antagónica del probiótico vs antibiótico con su interacción competitiva. Se utilizaron pruebas API CHL 50 para la identificación de las BAL que se complementaron con la producción de gas, actividad de catalasa y viabilidad a diferentes concentraciones de sales biliares y temperaturas. Resultados. El plan de análisis permitió seleccionar dos aislados que se identificaron como: Lactobacillus plantarum 1 H1 y Lb. plantarum 1 H2. Las pruebas microbiológicas evidenciaron que las BAL seleccionadas inhibieron principalmente los enteropatógenos: Escherichia coli, Salmonella typhimurium y Clostridium perfringens. Las pruebas bioquímicas fueron compatibles con el género Lactobacillus, mostrando el siguiente perfil: Catalasa negativas; no productoras de gas; productoras de isómeros DL; resistentes a sales biliares y pH óptimo de crecimiento 3.5 a 38oC. Conclusiones. Los dos aislados de Lactobacillus plantarum 1 reunieron las características para el uso potencial como probióticos. Se recomienda su uso como complemento de terapias con antibióticos y en la elaboración de inóculos para aplicación en raciones alimenticias de lechones destetos, sin embargo, estudios complementarios se requieren para evaluar el efecto directo del probiótico como sustituto del uso de antibióticos in vivo.

Aloe vera Aqueous Extract Effect on Morphine Withdrawal Syndrome in Morphine-Dependent Female Rats.

Science.gov (United States)

Shahraki, Mohammad Reza; Mirshekari, Hamideh; Sabri, Azame

2014-09-01

Aloe vera is a medicinal herb used as an anti-inflammatory and sedative agent. The current study aimed to evaluate the effect of Aloe vera aqueous extract on morphine withdrawal symptoms in morphine-dependent female rats. The current research was performed on 40 female Wista-Albino rats which were made dependent on morphine using Houshyar protocol and were randomly divided into five groups (A, B, C, D, and E). Group A did not receive any agent in the period of handling but other groups (B, C, D and E) received 5, 10, 20 and 40 mg/kg of Aloe vera aqueous extract by gavage, three times daily for a week, respectively. Withdrawal symptoms, stool form, agitation, disparity, floppy eyelids, and body mass variations were checked for 10 days. The obtained data were analyzed using SPSS v.11 software, and Friedman, Kruskal-Wallis, and Mann-Whitney statistical tests. Statistical difference was considered significant (P E were significantly higher than those of others groups; however, these symptoms in group C were significantly lower than those of the other groups. The results of the present study revealed that the Aloe vera aqueous extract had various effects on morphine withdrawal syndrome in morphine-dependent female rats .

Effect of Aloe vera on some indicators of cell damage after a period of aerobic exercise in male athletes

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Barati Amir-Hosein

2015-07-01

Full Text Available Introduction: Aloe vera is a medicinal plant with antioxidant activity. The purpose of this study was to determine the effect of Aloe vera on some indicators of cell damage after a period of aerobic exercise in male athletes of 15-18 years old. Methods: In this clinical trial study, 20 male students with average weight of 64.85 ± 51 and height of 172.05 ± 6.4 were randomly assigned to 2 groups of Aloe vera supplement (n = 10 and placebo (n = 10. Then aerobic training was conducted for 4 weeks. The supplemented group took 3 capsules, each capsule contains 2 g of dried Aloe vera and placebo group took 3 capsules/day containing dextran after every meal. To determine the index of cell injury markers creatine kinase (CK, lactate dehydrogenase (LDH and reactive protein C (CRP were determined and blood samples were collected 24 hours before and after each test. The data were analyzed using ANOVA and independent t test. Significance level was considered as P ≤ .05. Results: The use of Aloe vera during aerobic exercise significantly reduced LDH (P = .006 in the supplement group (15% reduction compared to placebo group and CRP with 11% reduction in the supplement group compared to placebo (P = .008. There was no significant reduction in CK. Conclusion: The findings of this study showed that Aloe vera reduces cell damage and inflammation indicators. This result may reflect the role of Aloe vera as anti-inflammatory and antioxidant agent.

Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya

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Utari Kresnoadi

2014-03-01

Full Text Available Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb. As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan

Efficient protocol for rapid Aloe vera micropropagation.

Science.gov (United States)

Molsaghi, Mozhgan; Moieni, Ahmad; Kahrizi, Danial

2014-06-01

Aloe vera Linn. (Liliaceae) is a medicinal plant and has a number of curative properties. Vegetative propagation has not enough potential for supplying market demand. However, via in vitro propagation makes possible the mass production of Aloe plants. The current study was conducted to investigate growth regulators' effects on proliferation of A. vera. In this study, for comparison of plant growth regulators' effects on proliferation, the shoot tips and auxiliary buds of A. vera were cultured in the Murashige and Skoog (MS) medium. Rooted plantlets were transferred to garden soil, compost, and sand in the proportion of 1:1:1, respectively, after hardening. The maximum number of shoots was obtained on the medium supplemented with 1 mg/L IAA+4 mg/L BAP and 0.2 mg/L IAA+0.8 BAP mg/L. Rooting was also achieved in the same media composition proliferation of shoot. The acclimatized plants showed 100% of survival. The regenerated plants looked healthy, and they were morphologically similar to that of stock plants. These results suggest that in vitro culture may be used as a technique for rapid propagation of A. vera.

Anti-hyperglycemic effect of Aloe vera peel extract on blood sugar level of alloxan-induced Wistar rats

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Peniati, E.; Setiadi, E.; Susanti, R.; Iswari, R. S.

2018-03-01

Aloe vera peel contains flavonoids, alkaloids, tannins, saponins, and sterols as its secondary metabolites. This research explores the effect of Aloe vera peel extract on blood glucose levels of alloxan-induced Wistar rats in a laboratory experimental scale. Blood glucose examination was performed by using GOD-PAP method. Twenty five 2 months old-white rat (Rattus norvegicus) male wistar strain weigh 150-200 grams body weight, and in healthy condition, was randomly divided into five groups. Those five groups were negative control group (K-), positive control group (K+), treatment group 1 (P1), treatment group 2 (P 2), and treatment group 3 (P 3). Each group was fed by standard diet and ad-libitum drinking. Treatments were given for 28 days. On the day 29, blood glucose level of all groups were analyzed. The results showed that the highest blood glucose levels in control group rat were positive (191.2 mg/dl). Aloe vera extract was able to decrease blood sugar level up to 104,6mg/dl in P3 group treatment rats (served Aloe vera extract 350 mg/kg BW/day). It comes to the conclusion that giving Aloe vera peel extract for 28 days decreases blood sugar level of hyperglycemic rat.

Transforming growth factor-β (TGF-β) activation in cutaneous wounds after topical application of aloe vera gel.

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Takzaree, Nasrin; Hadjiakhondi, Abbas; Hassanzadeh, Gholamreza; Rouini, Mohammad Reza; Manayi, Azadeh; Zolbin, Masoumeh Majidi

2016-12-01

Aloe vera is a medicinal plant used to treat various skin diseases. The effects of using aloe vera gel on the healing process were investigated by microscopic methods, cell counting, and TGF-β gene expression in the wound bed. Sixty Wistar rats weighing 200-250 g were placed under anesthesia in sterile conditions. A square 1.5 cm × 1.5 cm wound was made on the back of the neck. The rats were divided into control and 2 experimental groups. Additionally, the control and experimental groups were separated into 3 subgroups corresponding to 4, 7, and 14 days of study. In the first experimental group, aloe vera was used twice on the wound. The second experimental group received aloe vera overtreatment once on the wound. The positive control group received daily application of 1% phenytoein cream following surgical wound creation. The control group did not receive any treatment. This tissue was examined using histological staining (H&E) and Masson's Trichrome. Wound surface and wound healing were evaluated separately. TGF-β gene expression was analyzed by RT-PCR. Results showed that fibroblasts in both experimental groups were significantly increased, thereby acceleration wound healing. Application of aloe vera gel will increase TGF-β gene expression, ultimately accelerating the wound healing process.

Radioprotective effects of Aloe vera leaf extract on skin of Swiss mice after gamma irradiation

International Nuclear Information System (INIS)

Gehlot, Prashasnika; Saini, M.R.

2004-01-01

Full text: Biological effects of radiation are detrimental to life. Skin being a cell-renewal system is one of the best organ for studying radiation induced effects and their modulation by antioxidants. An attempt has been made to evaluate radioprotective efficacy of Aloe vera leaf extract on skin in Swiss mice (1g/kg body wt/day). The mice selected from inbreed colony were divided into two groups. The first group was given Aloe vera extract orally for 15th consecutive days and served as experimental group while the other group received DDW (vol. equal to Aloe extract) to serve as control group. On the 15th day, after 30 min of above treatment animals of both the groups were exposed to 2 Gy gamma irradiation and autopsied on 6h 1, 3, 7, 14 and 21 days. DNA as well as total protein decreases in control group as compared to the normal value. Surprisingly, in experimental group, DNA and protein increases in comparison to the control group. Thus, Aloe vera were found to have positive influence against radiation induced alterations on skin of Swiss albino mice

Effect of Aloe vera on glycaemic control in prediabetes and type 2 diabetes: a systematic review and meta-analysis.

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Suksomboon, N; Poolsup, N; Punthanitisarn, S

2016-04-01

Aloe vera (Aloe vera (L.) Burm.f., Xanthorrhoeaceae family) has long been used in folk or traditional medicine for diabetes. Several studies have been conducted on the effect of Aloe vera on glycaemic control, but the results appear inconsistent. We undertook a systematic review and meta-analysis to assess the effect of Aloe vera on glycaemic control in prediabetes and type 2 diabetes. A comprehensive literature search was conducted through MEDLINE, CENTRAL, CINAHL, Scopus, http://clinicaltrials.gov, Web of Science, Proquest, LILACS, HerbMed, NAPRALERT and CNKI to the end of January 2016 without language restriction. Historical search of relevant articles and personal contact with experts in the area were also undertaken. Studies were included if they were (1) randomized controlled trials of Aloe vera aimed at assessing glycaemic control in prediabetes or type 2 diabetes and (2) reporting fasting plasma glucose (FPG) or haemoglobin A1c (HbA1c ). Treatment effect was estimated with mean difference in the final value of FPG and HbA1c between the treatment and the control groups. Eight trials involving 470 patients (235 each for prediabetes and type 2 diabetes) were included. In prediabetes, Aloe vera significantly improved FPG (mean difference -0·22 mmol/L, 95% CI -0·32 mmol/L to -0·12 mmol/L, P Aloe vera may improve glycaemic control in type 2 diabetes, with a marginal improvement in FPG (mean differences -1·17 mmol/L, 95% CI -2·35 mmol/L to 0·00 mmol/L, P = 0·05) and a significant improvement in HbA1c (mean difference -11 mmol/mol, 95% CI -19 mmol/mol to -2 mmol/mol, P = 0·01). The current evidence suggests some potential benefit of Aloe vera in improving glycaemic control in prediabetes and type 2 diabetes. However, given the limitations of the available evidence and the high heterogeneity in study results, high-quality, well-powered randomized controlled trials using standardized preparations are needed to quantify any beneficial effects of Aloe vera on

Identification of phytochemical components of aloe plantlets by gas ...

African Journals Online (AJOL)

MRT Pack 20 DVDs

2013-12-04

Dec 4, 2013 ... mented with 0.5 mgl-1 benzyl adenine + 0.5 mgl-1 α-naphthalene acetic acids into ... 5973) coupled to H.P. gas chromatograph (model 6890) equipped .... effects of Aloe vera leaf gel extract on oxidative stress in rats treated.

Comparative Efficacy of Aloe vera and Benzydamine Mouthwashes on Radiation-induced Oral Mucositis: A Triple-blind, Randomised, Controlled Clinical Trial.

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Sahebjamee, Mahnaz; Mansourian, Arash; Hajimirzamohammad, Mohammad; Mohammad, Haji Mirza Mohammad; Zadeh, Mohsen Taghi; Bekhradi, Reza; Kazemian, Ali; Manifar, Soheila; Ashnagar, Sajjad; Doroudgar, Kiavash

2015-01-01

To compare the efficacy of an Aloe vera mouthwash with a benzydamine mouthwash in the alleviation of radiation- induced mucositis in head and neck cancer patients using a triple-blind, randomised controlled trial. Twenty-six eligible head and neck cancer patients who were to receive conventional radiation therapy at the radiation oncology department were randomised to receive an Aloe vera mouthwash or a benzydamine mouthwash. Mucositis severity was assessed during the course of radiation therapy using the WHO grading system. At baseline, there was no difference in the distribution of mucositis severity between the two groups. The mean interval between radiation therapy and onset of mucositis was similar for both groups (Aloe vera 15.69±7.77 days, benzydamine 15.85±12.96 days). The mean interval between the start of radiation therapy and the maximum severity of mucositis were was also similar in both the Aloe vera and benzydamine groups (Aloe vera 23.38±10.75 days, benzydamine 23.54±15.45 days). Mean changes of mucositis severity over time in both groups were statistically similar and the effect of both treatments did not change signficantly with time (p=0.09). Aloe vera mouthwash was as beneficial as benzydamine mouthwash in alleviating the severity of radiation-induced mucositis and showed no side effects. The Aloe vera mouthwash could be an alternative agent in the treatment of radiation-induced mucositis in patients with head and neck cancers.

Effect of Gamma Irradiation and Aloe vera on Antioxidant Status and Thyroid Functions in Female Rats

International Nuclear Information System (INIS)

El-Sherbiny, E. M.

2008-01-01

The aim of this research is to evaluate the antioxidant status and thyroid functions of female albino rats (Rattus norvegicus) when exposed to 3.0 Gy of gamma ray (dose rats = 0.696 Gy/min.) as s single dose and the role of 0.25 ml Aloe vera whole leaf juice filtrate/kg body weight against the damage caused by gamma irradiation. Total number of 50 female albino rats were equally divided into 5 groups; normal control group, irradiated group, Aloe vera administered group, irradiated rats followed by Aloe vera administration for 1 week and the 5th group is the irradiated rats followed by Aloe vera administration for 2 weeks starting from 24 h post-irradiation. Total antioxidant capacity, thiobarbituric acid reactive substances (TBARs), triiodothyronine (T3) and thyroxine (T4) were measured in serum of all groups. The results of this study revealed that 3.0 Gy of gamma irradiation resulted in a highly significant reduction in serum total antioxidant capacity (39.89%), highly significant increase in TBARs (29.19%) and a significant increase in serum T3 and T4 levels (8.21 and 25.51%, respectively) compared to control group. There was a non-significant change in serum total anti-oxidant capacity and TBARs, and a highly significant decrease in serum T3 and T4 levels (31.00 and 36.57%) in rats administered Aloe vera alone. Concerning rats administered Aloe vera whole leaf juice filtrate post-irradiation, serum total anti-oxidant capacity and TBARs were restored to the normal levels after 2 weeks. Serum levels of T3 and T4 (represent thyroid functions) were restored to the normal levels after 1 week and found to be inhibited (20.41 and 22.62%, respectively) after 2 weeks of administration. (author)

Millimeter Detection of AlO (X 2Σ+): Metal Oxide Chemistry in the Envelope of VY Canis Majoris

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Tenenbaum, E. D.; Ziurys, L. M.

2009-03-01

A new circumstellar molecule, the radical AlO (X 2Σ+), has been detected toward the envelope of the oxygen-rich supergiant star VY Canis Majoris (VY CMa) using the Arizona Radio Observatory (ARO). The N = 7 → 6 and 6 → 5 rotational transitions of AlO at 268 and 230 GHz were observed at 1 mm using the ARO Submillimeter Telescope (SMT) and the N = 4 → 3 line was detected at 2 mm using the ARO 12 m telescope. Based on the shape of the line profiles, AlO most likely arises from the dust-forming region in the spherical outflow of VY CMa, as opposed to the blue or redshifted winds, with a source size of θ s ~ 0farcs5. Given this source size, the column density of AlO was found to be N tot ~ 2 × 1015 cm-2 for T rot ~ 230 K, with a fractional abundance, relative to H2, of ~10-8. Gas-phase thermodynamic equilibrium chemistry is the likely formation mechanism for AlO in VY CMa, but either shocks disrupt the condensation process into Al2O3, or chemical "freezeout" occurs. The species therefore survives further into the circumstellar envelope to a radius of r ~ 20 R *. The detection of AlO in VY CMa is additional evidence of an active gas-phase refractory chemistry in oxygen-rich envelopes, and suggests such objects may be fruitful sources for other new oxide identifications.

Alo Streimann pääses Eesti Posti juhatusse suunatud konkursiga / Tõnis Arro ; interv. Toomas Kümmel

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Arro, Tõnis

2003-01-01

Personaliotsingufirma Fontes nõukogu esimees Tõnis Arro tunnistab, et Eesti Posti juhi kohalt tagasiastunud Alo Streimanni ametisse valimisel oli tunda ettevõtte eelmise nõukogu soovi suruda sellele ametikohale Keskerakonnale lähedalseisev inimene. Vt. samas: Aastatagused arvamused Alo Streimannist: Kalev Kaal, Kostel Gerndorf, Küllo Arjakas

Antioxidant activity of the exudate from Aloe barbadensis leaves in ...

African Journals Online (AJOL)

Dr. J. T. Ekanem

... peroxidation products levels. Keywords: Diabetes, Aloe barbadensis, oxidative stress, Antioxidant enzymes ... Many minor components of foods, such as secondary plant ... different doses of the extract to 5 groups of rats. (6 rats per group).

De estructuras, culturas y otros demonios: una revisión de las insurrecciones andinas de finales del siglo XVIII

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Hanni Jalil Paier

2011-07-01

Full Text Available Este ensayo observa las insurrecciones populares del siglo XVIII no como episodios casuales o aislados, sino como síntomas de un sentimiento generalizado de descontento social e intensificado conflicto. Sentimientos cuya intensidad se incrementó durante la segunda mitad del siglo XVIII y culminó con la Gran Rebelión de 1780-1783 en el sur de los Andes, el levantamiento en Quito de 1765 y la Rebelión de los Comuneros en la Nueva Granada de 1781. Este artículo examina los trabajos que hacen referencia a estas revueltas, reconociendo el hecho que la producción académica de las insurrecciones de finales del siglo XVIII en las colonias americanas forman parte de los amplios debates académicos que abordan las insurrecciones fuera del contexto Andino, e incorpora preguntas promulgadas por los estudiosos de las revueltas campesinas y el conflicto armado en otros campos y otros periodos.

Effectiveness of Aloe Vera gel compared with 1% silver sulphadiazine cream as burn wound dressing in second degree burns.

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Shahzad, Muhammad Naveed; Ahmed, Naheed

2013-02-01

To assess the efficacy of Aloe Vera gel compared with 1% silver sulfadiazine cream as a burn dressing for the treatment of superficial and partial thickness burns. This Interventional Comparative study was carried out at the Burn unit and Plastic surgery department, Nishtar Hospital Multan, Pakistan from July 2008 to December 2010. A total of 50 patients with superficial and partial thickness burns were divided into two equal groups randomly by consecutive sampling method, one group was dressed with Aloe Vera gel while the other was treated with 1% silversulphadiazine cream, and the results regarding duration of wound epithelialization, pain relief and cost of treatment were compared. In patients treated with Aloe Vera gel, healing of burn wounds were remarkably early than those patients treated with 1% silver sulfadiazine. All the patients of Aloe Vera group were relieved of pain earlier than those patients who were treated with SSD. Thermal burns patients dressed with Aloe Vera gel showed advantage compared to those dressed with SSD regarding early wound epithelialization, earlier pain relief and cost-effectiveness.

Characterization of mangrove forest types based on ALOS-PALSAR in overall Indonesian archipelago

International Nuclear Information System (INIS)

Darmawan, S; Takeuchi, W; Vetrita, Y; Winarso, G; Wikantika, K; Sari, D K

2014-01-01

Indonesia has largest mangrove forest in the world, total area around 3.5 million ha or 17% – 23% from mangrove forest in the world. Mangrove forest provides products and services, such as carbon balance of the coastal zone. Mapping and monitoring biomass of mangrove is very important but field survey of mangrove biomass and productivity in overall Indonesia is very difficult. Global-scale mosaics with HH and HV backscatter of Advanced Land Observing Satellite (ALOS) Phased Array L-band Synthetic Aperture Radar (PALSAR) which is 50-m spatial resolution has been generated. This image available for identification and monitoring mangrove forest. The Objective of this research to investigate characterization of mangrove forest types based on ALOS-PALSAR in overall Indonesian archipelago. Methodology consists of collecting ALOS-PALSAR image for overall Indonesian archipelago, preprocessing and mosaicking, collecting region of interest of mangrove forest, plotting, ground survey, characterization and classification. The result of this research has showed characterization of mangrove forest types based on ALOS-PALSAR. Indonesian mangrove forest types has HH value around -10 dB until -7 dB and HV value around -17 dB until -13 dB. Higher of HH and HV backscatters value indicated higher of level biomass. Based on scatter plot of HH and HV, Indonesian mangrove forest can be classified in three level biomass. Generally level biomass of mangrove forest in Indonesia archipelago is moderate

Effects of plant sterols derived from Aloe vera gel on human dermal fibroblasts in vitro and on skin condition in Japanese women

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Tanaka M

2015-02-01

Full Text Available Miyuki Tanaka,1 Eriko Misawa,1 Koji Yamauchi,1 Fumiaki Abe,1 Chiaki Ishizaki2 1Functional Food Research Department, Food Science and Technology Institute, Morinaga Milk Industry Co, Ltd, Zama, Kanagawa, 2Ebisu Skin Research Center, Inforward, Inc., Tokyo, Japan Background: Aloe is known for its topical use for treating wounds and burns. Many previous studies reported the healing effects of Aloe vera. However, there are few clinical studies on the effect of orally administered A. vera gel on the skin. Aloe sterols are a type of plant sterols that have the capability to regulate the metabolism of glucose and lipids. In a recent study, we confirmed that ingested Aloe sterols reached the peripheral tissues through the bloodstream. However, their influence on dermal fibroblasts has not been investigated. Methods: First, we investigated the capability of Aloe sterols (cycloartenol and lophenol to stimulate human dermal fibroblasts in vitro. Then, we investigated the effect of intake of Aloe vera gel powder (AVGP containing 40 µg Aloe sterols on the skin conditions in Japanese women with dry skin in a randomized, double-blind, placebo-controlled trial. Results: After cocultivation with Aloe sterols, the production of collagen and hyaluronic acid increased by approximately two-fold and 1.5-fold, and gene expression levels of these enzymes responsible for their synthesis were also observed in human dermal fibroblasts. An increase in arm skin hydration was observed at 8 weeks in the AVGP group, whereas a slight decrease in arm skin hydration was noted in the placebo group. However, there was no statistical difference between AVGP and placebo groups in skin moisture. In subgroup analysis, the change in the mean wrinkle depth was significantly lower in the AVGP group than in the control group. In addition, percent body fat after 8 weeks was significantly lower in the AVGP group. No AVGP intake-dependent harmful phenomenon was observed during the intake

Comparative antimicrobial activities of aloe vera gel and leaf ...

African Journals Online (AJOL)

The comparative antimicrobial activities of the gel and leaf of Aloe vera were tested against Staphylococcus aureus, Pseudomonas aeruginosa, Trichophyton mentagraphytes, T. schoeleinii, Microsporium canis and Candida albicans. Ethanol was used for the extraction of the leaf after obtaining the gel from it. Antimicrobial ...

Lack of Prophylactic Effects of Aloe Vera Gel on Radiation Induced Dermatitis in Breast Cancer Patients

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Ahmadloo, Niloofar; Kadkhodaei, Behnam; Omidvari, Shapour; Mosalaei, Ahmad; Ansari, Mansour; Nasrollahi, Hamid; Hamedi, Sayed Hasan; Mohammadianpanah, Mohammad

2017-04-01

Purpose: Radiotherapy induced dermatitis is a common and sometimes serious side effect. We conducted a randomized study to understand whether the adjunctive use of aloe vera gel might reduce the prevalence and/or severity of radiotherapy induced dermatitis. Methods: One hundred patients with newly diagnosed breast cancer were randomized to receive aloe vera gel or nothing during adjuvant radiotherapy consisting of conventional external beam radiation using 6 MV mega voltage linear accelerator photons. The radiation portals were composed of breast fields in all patients and supraclavicular and posterior axillary fields in node positive cases. The total dose was 50 Gy with a daily fraction of 2 Gy, five fractions per week. Results: Dermatitis was first found among patients of both groups after week 2. In the aloe vera and control groups, 2/8 patients and 5/12 patients had dermatitis grade 1 after weeks 2 (P value = 0.240) and 3 (P value = 0.317). After the 4th week, the numbers were 18 and, 23 fordermatitis grade 1 and only 1 for grade 3 dermatitis (P value = 0.328). After the 5th week, 31, 12 and 2 patients in the aloe vera group and 36, 6 and 5 of the controls had grades 1, 2 and 3 dermatitis, respectively (P value = 0.488). Conclusion: Aloe vera exerted no positive effect on prevalence orseverity of radiation dermatitis in this study. Creative Commons Attribution License

Effectiveness of Aloe Vera and Antioxidant along with Physiotherapy in the Management of Oral Submucous Fibrosis.

Science.gov (United States)

Singh, Nisha; Hebbale, Manjula; Mhapuskar, Amit; Ul Nisa, Shams; Thopte, Shameeka; Singh, Saumya

2016-01-01

Oral submucous fibrosis (OSMF) is a well-established precancerous condition affecting the oral mucosa. It is a disease that causes significant morbidity (in terms of loss of mouth function as tissues become rigid and mouth opening becomes difficult) and mortality (when transformation into squamous cell carcinoma occurs). The aim of the study is to compare the efficacy of Aloe vera with antioxidant when given along with physiotherapy in the management of OSMF. Forty patients presenting with clinical signs and symptoms of OSMF were included for the study after informed consent. Group A included 20 patients who received Aloe vera gel (forever living gel) along with physiotherapy. Group B included 20 patients who received antioxidant capsules twice daily for 3 months along with physiotherapy exercises four times in a day. The following parameters, that is, burning sensation, mouth opening, tongue protrusion and cheek flexibility were recorded at each visit. Majority of the participant enrolled were in the age range of 30 to 35 years. Improvement in all the parameters was seen with the individuals receiving Aloe vera gel in comparison to antioxidants. So, Aloe vera being a soothing, simple and safe mode of treatment along with proper habit restriction can be considered to be an effectual protocol in the management of OSMF. The analgesic effects of Aloe vera with the physiotherapy exercises provide better results in reducing burning sensation and improving mouth opening, tongue protrusion and cheek flexibility in comparison to antioxidants.

Cytotoxic Compounds from Aloe megalacantha

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Negera Abdissa

2017-07-01

Full Text Available Phytochemical investigation of the ethyl acetate extract of the roots of Aloe megalacantha led to the isolation of four new natural products—1,8-dimethoxynepodinol (1, aloesaponarin III (2, 10-O-methylchrysalodin (3 and methyl-26-O-feruloyl-oxyhexacosanate (4—along with ten known compounds. All purified metabolites were characterized by NMR, mass spectrometric analyses and comparison with literature data. The isolates were evaluated for their cytotoxic activity against a human cervix carcinoma cell line KB-3-1 and some of them exhibited good activity, with aloesaponarin II (IC50 = 0.98 µM being the most active compound.

Aloe sterol supplementation improves skin elasticity in Japanese men with sunlight-exposed skin: a 12-week double-blind, randomized controlled trial

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Tanaka M

2016-11-01

Full Text Available Miyuki Tanaka,1 Yuki Yamamoto,2 Eriko Misawa,1 Kazumi Nabeshima,1 Marie Saito,1 Koji Yamauchi,1 Fumiaki Abe,1 Fukumi Furukawa2 1Functional Food Ingredients Department, Food Ingredients & Technology Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa, 2Department of Dermatology, Wakayama Medical University, Kimiidera, Wakayama, Japan Background/objective: Recently, it was confirmed that the daily oral intake of plant sterols of Aloe vera gel (Aloe sterol significantly increases the skin barrier function, moisture, and elasticity in photoprotected skin. This study aimed to investigate whether Aloe sterol intake affected skin conditions following sunlight exposure in Japanese men. Methods: We performed a 12-week, randomized, double-blind, placebo-controlled study to evaluate the effects of oral Aloe sterol supplementation on skin conditions in 48 apparently healthy men (age range: 30–59 years; average: 45 years. The subjects were instructed to expose the measurement position of the arms to the sunlight outdoors every day for 12 weeks. The skin parameters were measured at 0 (baseline, 4, 8, and 12 weeks. Results: Depending on the time for the revelation of the sunlight, the b* value and melanin index increased and the skin moisture decreased. After taking an Aloe sterol tablet daily for 12 weeks, the skin elasticity index (R2, R5, and R7 levels were significantly higher than the baseline value. There were no differences between the groups in these skin elasticity values. In the subgroup analysis of subjects aged <46 years, the change in the R5 and R7 was significantly higher in the Aloe group than in the placebo group at 8 weeks (P=0.0412 and P=0.0410, respectively. There was a difference in the quantity of sun exposure between each subject, and an additional clinical study that standardizes the amount of ultraviolet rays is warranted. No Aloe sterol intake-dependent harmful phenomenon was observed during the intake period

Dietary aloe vera gel powder and extract inhibit azoxymethane- induced colorectal aberrant crypt foci in mice fed a high- fat diet.

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Chihara, Takeshi; Shimpo, Kan; Kaneko, Takaaki; Beppu, Hidehiko; Higashiguchi, Takashi; Sonoda, Shigeru; Tanaka, Miyuki; Yamada, Muneo; Abe, Fumiaki

2015-01-01

Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

Estudio de actinomicetos marinos aislados de la costa central del Perú y su actividad antibacteriana frente a Staphylococcus aureus Meticilina Resistentes y Enterococcus faecalis Vancomicina Resistentes Study of marine actinomycetes isolated from the central coast of Peru and their antibacterial activity against Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococcus faecalis

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Jorge León

2011-06-01

Full Text Available Objetivos. Determinar el potencial antimicrobiano de actinomicetos marinos frente a cepas S. aureus meticilino-resistentes (MRSA y E. faecalis vancomicina-resistentes (VRE. Materiales y métodos. En dos medios de cultivo se sembraron 29 cepas de actinomicetos aislados de sedimento marino. Se evaluó la capacidad inhibitoria mediante pruebas de antagonismo in vitro para MRSA y VRE. Se procesó los extractos orgánicos de tres actinomicetos seleccionados para determinar la Concentración Mínima Inhibitoria (CMI del compuesto activo. Resultados. La mayoría de los actinomicetos aislados correspondieron a un grupo homogéneo de blanco-grisáceos (62% con buen nivel de crecimiento en agar marino. Los porcentajes inhibitorios fueron superiores a 85% para ambos patógenos con halos de inhibición mayores a 69 y 78 mm de diámetro para MRSA y VRE respectivamente. Los extractos diclorometánicos de tres de los actinomicetos aislados (I-400A, B1-T61, M10-77 mostraron gran potencial inhibitorio de ambos patógenos, siendo M10-77 la cepa de actinomiceto de mayor actividad antibiótica frente a S. aureus ATCC 43300 resistente a meticilina y E. faecalis ATCC 51299 resistente a vancomicina con una Concentración Mínima Inhibitoria (CMI de 7,9 y 31,7 μg/ mL respectivamente. El análisis filogenético de la cepa M10- 77 presenta un 99% de similaridad con la especie marina Streptomyces erythrogriseus. Conclusiones. El sedimento marino de la costa central del Perú es fuente promisorio de cepas de actinomicetos con gran capacidad de producir compuestos bioactivos capaces de inhibir patógenos tipificados como multidrogo-resistentes tales como S. aureus meticilino resistentes y E. faecalis vancomicina resistentes.Objectives. To determine the antimicrobial potential of marine actinomycetes against drug-resistant pathogens represented by strains of methicillin-resistant Staphylococcus aureus (MRSA and vancomycin-resistant Enterococcus faecalis (VRE. Materials and

Detección de virus herpes canino tipo 1 en Chile

OpenAIRE

NAVARRO, C; CELEDON, M; PIZARRO, J

2003-01-01

RESUMEN Este trabajo informa la detección del virus herpes canino tipo 1 (VHC-1) en nuestro país, confirmando la fuerte sospecha clínica de su existencia. Se logró obtener un aislado viral, denominado RP5, a partir de casos clínicos diagnosticados como enfermedad hemorrágica de los cachorros. Este aislado inoculado en monocapas celulares produce el típico efecto citopático de miembros de la subfamilia alphaherpesvirinae, familia Herpesviridae: lisis celular a tiempos cortos. Este aislado mani...

Detección de virus herpes canino tipo 1 en Chile Canine herpesvirus-1 detection in Chile

OpenAIRE

C NAVARRO; M CELEDON; J PIZARRO

2003-01-01

RESUMEN Este trabajo informa la detección del virus herpes canino tipo 1 (VHC-1) en nuestro país, confirmando la fuerte sospecha clínica de su existencia. Se logró obtener un aislado viral, denominado RP5, a partir de casos clínicos diagnosticados como enfermedad hemorrágica de los cachorros. Este aislado inoculado en monocapas celulares produce el típico efecto citopático de miembros de la subfamilia alphaherpesvirinae, familia Herpesviridae: lisis celular a tiempos cortos. Este aislado mani...

MILLIMETER DETECTION OF AlO (X 2Σ+): METAL OXIDE CHEMISTRY IN THE ENVELOPE OF VY CANIS MAJORIS

International Nuclear Information System (INIS)

Tenenbaum, E. D.; Ziurys, L. M.

2009-01-01

A new circumstellar molecule, the radical AlO (X 2 Σ + ), has been detected toward the envelope of the oxygen-rich supergiant star VY Canis Majoris (VY CMa) using the Arizona Radio Observatory (ARO). The N = 7 → 6 and 6 → 5 rotational transitions of AlO at 268 and 230 GHz were observed at 1 mm using the ARO Submillimeter Telescope (SMT) and the N = 4 → 3 line was detected at 2 mm using the ARO 12 m telescope. Based on the shape of the line profiles, AlO most likely arises from the dust-forming region in the spherical outflow of VY CMa, as opposed to the blue or redshifted winds, with a source size of θ s ∼ 0.''5. Given this source size, the column density of AlO was found to be N tot ∼ 2 x 10 15 cm -2 for T rot ∼ 230 K, with a fractional abundance, relative to H 2 , of ∼10 -8 . Gas-phase thermodynamic equilibrium chemistry is the likely formation mechanism for AlO in VY CMa, but either shocks disrupt the condensation process into Al 2 O 3 , or chemical 'freezeout' occurs. The species therefore survives further into the circumstellar envelope to a radius of r ∼ 20 R * . The detection of AlO in VY CMa is additional evidence of an active gas-phase refractory chemistry in oxygen-rich envelopes, and suggests such objects may be fruitful sources for other new oxide identifications.

Memristors in the electrical network of Aloe vera L.

Science.gov (United States)

Volkov, Alexander G; Reedus, Jada; Mitchell, Colee M; Tucket, Clayton; Forde-Tuckett, Victoria; Volkova, Maya I; Markin, Vladislav S; Chua, Leon

2014-01-01

A memristor is a resistor with memory, which is a non-linear passive two-terminal electrical element relating magnetic flux linkage and electrical charge. Here we found that memristors exist in vivo. The electrostimulation of the Aloe vera by bipolar sinusoidal or triangle periodic waves induce electrical responses with fingerprints of memristors. Uncouplers carbonylcyanide-3-chlorophenylhydrazone and carbonylcyanide-4-trifluoromethoxy-phenyl hydrazone decrease the amplitude of electrical responses at low and high frequencies of bipolar periodic sinusoidal or triangle electrostimulating waves. Memristive behavior of an electrical network in the Aloe vera is linked to the properties of voltage gated ion channels: the K+ channel blocker TEACl reduces the electric response to a conventional resistor. Our results demonstrate that a voltage gated K+ channel in the excitable tissue of plants has properties of a memristor. The discovery of memristors in plants creates a new direction in the modeling and understanding of electrical phenomena in plants. PMID:25763487

Aloe vera extract as a promising treatment for the quality maintenance of minimally-processed table grapes

Directory of Open Access Journals (Sweden)

Giuseppina Rosaria Antonella Alberio

2015-06-01

Full Text Available The effect of an edible film obtained from a commercial Aloe vera extract, on the quality maintenance of minimally processed grapes belonging to three different cultivars (Sugar One, Victoria and Black Magic was evaluated by enzymatic (PPO, PME, β-GAL, physicochemical (pH, acidity, °Brix, and sensorial methods. All the analyzed parameters were measured in extracts obtained from minimally processed grapes packaged in ordinary atmosphere and stored at 4 °C for 15 days. Samples dipped into Aloe vera showed significant differences (p≤0.05 compared to untreated ones. The determination of such parameters and the evaluation of consumer acceptability were helpful to determine the effectiveness of the post-harvest treatment with Aloe vera for a storage period of 15 days.

Enhanced biocompatibility of ZnS:Mn quantum dots encapsulated with Aloe vera extract for therapeutic applications

International Nuclear Information System (INIS)

Anilkumar, M; Sneha Saj, A; Bindu, K R; Anila, E I

2016-01-01

Toxicity of nanoparticles remains to be a major issue in their application to the biomedical field. Aloe vera (AV) is one of the most widely exploited medicinal plants that have a multitude of amazing properties in the field of medicine. Methanol extract of Aloe vera can be used as a novel stabilising agent for quantum dots to reduce toxicity. We report the synthesis, structural characterization, antibacterial activity and cytotoxicity studies of ZnS:Mn quantum dots synthesized by the colloidal precipitation method, using methanol extract of Aloe vera (AVME) as the capping agent. The ZnS:Mn quantum dots capped with AVME exhibit superior performances in biocompatibility and antibacterial activity compared with ZnS:Mn quantum dots without encapsulation. (paper)

Sensibilidad in vitro de dos especies de Sclerotinia spp. y Sclerotium cepivorum a agentes de control biológico y fungicidas

OpenAIRE

Pérez-Moreno, Luis; Belmonte-Vargas, José Roberto; Núñez-Palenius, Héctor Gordon; Guzmán-Mendoza, Rafael; Mendoza-Celedón, Briseida

2015-01-01

Se evaluó la respuesta in vitro de dos aislados de Sclerotinia minor,cinco de S. sclerotiorum,y dos de Sclerotium cepivoruma 16 agentes biológicos y ocho fungicidas en un diseño experimental de parcelas divididas con arreglo factorial por cada patógeno. El factor A correspondió a los aislados del hongo y el factor B a los productos de control. La comparación de medias se realizó con la prueba de Tukey (P < 0.05). Se hicieron 11 evaluaciones cada 24 horas del crecimiento promedio radial miceli...

Clinical Effectiveness of Aloe Vera in the Management of Oral Mucosal Diseases- A Systematic Review

Science.gov (United States)

Nair, Gopakumar Ramachandran; Naidu, Giridhar Seetharam; Jain, Supreet; Makkad, Ramanpal Singh; Jha, Abhishek

2016-01-01

Introduction Aloe vera is well known for its medicinal properties which lead to its application in treating various diseases. Its use in treating oral lesions has not been much documented in literature. Aim Although, systematic reviews on aloe vera and its extracts have been done earlier, but in relation to oral diseases this is the first systematic review. The aim of the present systematic review was to compile evidence based studies on the effectiveness of Aloe vera in treatment of various oral diseases. Materials and Methods Computerized literature searches were performed to identify all published articles in the subject. The following databases were used: PUBMED [MEDLINE], SCOPUS, COCHRANE DATABASE, EMBASE and SCIENCE DIRECT using specific keywords. The search was limited to articles published in English or with an English Abstract. All articles (or abstracts if available as abstracts) were read in full. Data were extracted in a predefined fashion. Assessment was done using Jadad score. Results Fifteen studies satisfied the inclusion criteria. Population of sample study ranged from 20 patients to 110 patients with clinically diagnosed oral mucosal lesions. Out of 15 studies, five were on patients with oral lichen planus, two on patients with oral submucous fibrosis, other studies were carried on patients with burning mouth syndrome, radiation induced mucositis, candida associated denture stomatitis, xerostomic patients and four were on minor recurrent apthous stomatitis. Most studies showed statistically significant result demonstrating the effectiveness of Aloe vera in treatment of oral diseases. Conclusion Although there are promising results but in future, more controlled clinical trials are required to prove the effectiveness of Aloe vera for management of oral diseases. PMID:27656587

Propriedades farmacológicas da Aloe vera (L. Burm. f.

Directory of Open Access Journals (Sweden)

V.S. Freitas

2014-06-01

Full Text Available A Aloe vera (L. Burm.f. tem sido utilizada há milhares de anos na medicina tradicional para o tratamento de diversos males. O intuito desse trabalho foi o levantamento bibliográfico de artigos que evidenciassem a atividade farmacológica da Aloe vera. A revisão contemplou livros e periódicos nacionais e internacionais indexados nas bases de dados MEDLINE, LILACS e SciElo, nos idiomas português, inglês e espanhol, utilizando as palavras-chave citadas. Após o levantamento bibliográfico, constatou-se que várias atividades biológicas são atribuídas a Aloe vera. Evidências sugerem eficácia no tratamento da psoríase, herpes genital, queimaduras e hiperglicemia. Além disto, também foram demonstradas atividades antineoplásica, antimicrobiana, anti-inflamatória e imunomodulatória por estudos in vitro e in vivo, entretanto, na cicatrização de feridas, os resultados foram conflitantes. No tratamento de dermatite por radiação e em queimaduras solares sua eficácia não foi comprovada e foram relatados casos de hepatite aguda devido ao consumo de preparações orais. Tendo em vista as várias atividades comprovadas e poucos relatos acerca de sua contra indicação, conclui-se que o uso desta espécie corrobora o vasto uso popular.

Characterization of gelatin/chitosan scaffold blended with aloe vera and snail mucus for biomedical purpose.

Science.gov (United States)

López Angulo, Daniel Enrique; do Amaral Sobral, Paulo José

2016-11-01

Biologically active scaffolds used in tissue engineering and regenerative medicine have been generating promising results in skin replacement. The present study aims to test the hypothesis that the incorporation of Aloe vera and snail mucus into scaffolds based on gelatin and chitosan could improve their structure, composition and biodegradability, with a potential effect on bioactivity. Homogeneous pore diameter as well as pore walls in the composite scaffold could be seen in the SEM image. The pores in the scaffolds were interconnected and their sizes ranged from 93 to 296μm. The addition of Aloe vera and snail mucus enlarged the mean pore size with increased porosity and caused changes in the pore architecture. The FTIR analysis has shown good affinity and interaction between the matrix and the Aloe, which may decrease water-binding sites, so this fact hindered the water absorption capacity of the material. The mechanical properties could explain the highest swelling capacity of the snail scaffold, because the high percentage of elongation could facilitate the entry of liquid in it, generating a matrix with plenty of fluid retention. The real innovation in the present work could be the use of these substances (Aloe and snail mucus) for tissue engineering. Copyright © 2016 Elsevier B.V. All rights reserved.

The Protective Effect of Aloe Vera on Histological Structure of Endocrine Portion of Pancreas Gland in the Diabetic Rat

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N Erfani-Majd

2016-01-01

Full Text Available Introduction: Since aloe vera plant has many medical benefits, the present study aimed to investigate the protective effects of Aloe vera gel on the pancreatic islets and beta cells. Methods: This experimental study consisted of 50 mature male rats aged 2-3 months and weighed 200-250 g, who were randomly divided into five groups (n=10. Group I (control did not receive any treatments, and group II were diabetized via Streptozotocin (IP in 65 mg/kg, whose blood sample was taken after one week. Rats with blood glucose more than 250 mg/dL were considered as diabetic. Group III diabetic rats received the Aloe vera gel daily with dosage of 400 mg/kg, and group IV diabetic rats received insulin in 10 units/rat. Group V involved healthy rats which received only Aloe vera gel. After the last Aloe vera gel administration, blood glucose and body weight of all groups were measured on 15th and 30th days. Animals were euthanized with ether. Then tissues samples were collected from pancreas gland and fixed in 10% neutral buffered formalin solution. The 5-6 µ sections were made by paraffin embedding method and stained using haematoxylin-eosin (H&E and Aldehyde fuchsin stains. Ultimately, the histomorphometrical parameters were evaluated. Results: The mean number and size of pancreatic islets and beta cells of Langerhans islets decreased significantly in the diabetic group compared to the control group. The number of beta cells and diameter of langerhans islets increased significantly in the rats treated by Aloe vera gel in comparison to diabetic group at the end of 15th and 30th days. Conclusion: Applying Aloe vera gel seems to improve the renewal and restoration of langerhans islets and beta cells of pancreas gland in the diabetic rat.

Aloe vera is non-toxic to cells: A microculture tetrazolium technique colorimetric assay study

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Devi Gopakumar

2014-01-01

Full Text Available Introduction: Aloe vera (Av, a succulent of Liliaceae family is now a widely used medicinal plant. Its′ application covers a wide spectrum of human diseases, including oral mucosa, gastric mucosa and skin. Aloe vera preparations in the form of gel, mouth washes and cream are applied topically for many oral diseases. The applications include oral lichen planus, candidiasis, oral submucous fibrosis, geographic tongue, etc. Aims and Objectives: To evaluate the cytotoxicity of Av on human fibroblasts. Materials and Methods: Aloe vera preparation (70% was applied on the fibroblast cell lineage and the cell viability was evaluated by microculture tetrazolium technique (MTT colorimetric assay. Results: The cell viability at different concentrations was measured. The cells have maintained their viability at different concentrations used in the study. Conclusion: Our study shows the cell viability at different sample concentrations of Av. This could open up wide clinical applications of Av for reactive, inflammatory and potentially malignant oral and other mucocutaneous diseases.

Extent of Use of Aloe vera Locally Extracted Products for Management of Ailments in Communities of Kitagata Sub-county in Sheema District, Western Uganda

Science.gov (United States)

Adams, Kamukama; Eliot, Twineomujuni; Gerald, Agaba

2015-01-01

Aloe vera is widely used locally in communities in Uganda as a medicinal plant. It is said to contain various nutrient substances and vitamins that have curative properties. It is said to heal a variety of diseases in various communities. However the extent of use of this potential medicinal plant in Uganda and the various ailments for which it is used and the treatment outcomes are not clearly established and documented. In this cross-sectional study, carried out in August 2012 in Kitagata sub-county in Sheema district in western Uganda, data was collected from 131 randomly selected adult respondents using an interviewer administered semi-structured questionnaire. Key informants interviews and focused group discussions were also carried out with purposively selected participants. Data were collected on social demographic characteristics, practices and beliefs about Aloe vera. The data were analyzed using Excel version 2007 and Epi_Info software. To get the proportion of the community that use Aloe vera, the number of respondents that use aloe vera was expressed as a percentage of the total number of respondents. It was found out that all the respondents (100%) know aloe vera plant, 96.1% think that it can cure and 84.7% have ever used it. 90.9% of the respondents believe that Aloe vera is effective in curing ailments. 82.9% of these strongly believe in Aloe vera’s effectiveness. The diseases reported included malaria (31%), wounds (23%), abdominal pains (16%) and skin diseases (9%) among others. It was significantly noted that all the participants who had ever used Aloe vera still believe in it. 92.0% respondents reported that they can recommend aloe vera to a friend or relative. Only one participant strongly disagrees that Aloe vera has any curative properties and has never used it. PMID:26855960

Improving Hydrological Models of The Netherlands Using ALOS PALSAR

NARCIS (Netherlands)

Dekker, R.J.; Schuurmans, J.M.; Berendrecht, W.L.; Borren, W.; Ven, T.J.M. van de; Westerhoff, R.S.

2010-01-01

In this paper the improvement of the hydrological model metaSWAP of The Netherlands, with respect to soil moisture, is studied using remote sensing data. Therefore we investigate the value of ALOS PALSAR data of 2007 in combination with the method of Dubois et al. [1] for measuring volumetric

Influence de l'extrait aquueux de feuilles d'Aloe buettneri, Dicliptera ...

African Journals Online (AJOL)

Influence de l'extrait aquueux de feuilles d'Aloe buettneri, Dicliptera verticillata, Hibiscus macranthus et Justicia insularis sur la fertilité et quelques parametres biochemiques de la reproduction chez la rate.

Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge.

Science.gov (United States)

Gabriel, Ndakalimwe Naftal; Qiang, Jun; Ma, Xin Yu; He, Jie; Xu, Pao; Liu, Kai

2015-10-01

The current study investigated the effects of dietary Aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of GIFT-tilapia juveniles under Streptococcus iniae challenge. Five dietary groups were designed including a control and 100 % Aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. Fish fed dietary Aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced (P Aloe/kg feed compared to unsupplemented ones. High-density lipoprotein was significantly elevated in fish fed 0.5 and 1 % Aloe/kg feed compared to unsupplemented ones, and no significant changes (P > 0.05) were noted in low-density lipoprotein among test groups. Furthermore, high activities of superoxide dismutase, catalase, and glutathione peroxide in liver tissues were observed in Aloe-supplemented fish compared to unsupplemented ones, before and after S. iniae challenge (7.7 × 10(6) CFU cells/mL). Variations were also noted in malondialdehyde activity throughout the trial, but no significant difference (P > 0.05) was observed between groups. Meanwhile, Aloe-supplemented fish reduced serum aspartate and alanine aminotransferase (AST and ALT) activities before and after challenge. Based on the second-order polynomial regression analysis, dietary Aloe inclusion levels less than or equal to 1.88, 1.86, and 2.79 %/kg feed were determined to be suitable in improving plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia in this study, respectively. Thus, A. vera extracts may be recommended as a tilapia feed supplement to enhance fish antioxidant and hepatoprotective capacities, especially during disease outbreaks.

In vivo tracing of organophosphorus pesticides in cabbage (Brassica parachinensis) and aloe (Barbadensis)

International Nuclear Information System (INIS)

Qiu, Junlang; Chen, Guosheng; Zhou, Hong; Xu, Jianqiao; Wang, Fuxin; Zhu, Fang; Ouyang, Gangfeng

2016-01-01

In vivo solid-phase microextraction (SPME) sampling method coupled with gas chromatography–mass spectrometry (GC–MS) analysis was employed to trace the uptake and elimination of organophosphorus pesticides (OPPs) in two kinds of edible plants, cabbage (Brassica parachinensis) and aloe (Barbadensis). The metabolism of fenthion in aloe was also investigated by the liquid chromatography tandem mass spectrometry analysis (LC–MS/MS) to understand the fate of OPPs in living plants better. Transpiration stream concentration factor (TSCF) and depuration rate constants of the OPPs in living plants were obtained therein. The health risk of the OPPs treated aloe was estimated by the maximum residue limit (MRL) approach, and it revealed that the OPPs were rather safe for their fast degradable property. However, peak concentration of fenthion-sulfoxide was found to exceed the MRL and was higher than that of the parent fenthion, which indicated the potential risk of pesticide metabolites. This study highlighted the application of in vivo SPME for contaminant tracing in different living edible plants. The in vivo tracing method is very convenient and can provide more data to evaluate the risk of different pesticides, which are very important for the safety of agriculture production. - Highlights: • In vivo SPME was employed to sample organophosphorus pesticides in vegetables. • Uptake and elimination of OPPs were traced in cabbage and aloe. • In vivo tracing of fenthion demonstrated its metabolites could be rather dangerous. • The risks of OPPs were assessed based on the in vivo tracing data.

In vivo tracing of organophosphorus pesticides in cabbage (Brassica parachinensis) and aloe (Barbadensis)

Energy Technology Data Exchange (ETDEWEB)

Qiu, Junlang; Chen, Guosheng; Zhou, Hong; Xu, Jianqiao; Wang, Fuxin; Zhu, Fang, E-mail: ceszf@mail.sysu.edu.cn; Ouyang, Gangfeng, E-mail: cesoygf@mail.sysu.edu.cn

2016-04-15

In vivo solid-phase microextraction (SPME) sampling method coupled with gas chromatography–mass spectrometry (GC–MS) analysis was employed to trace the uptake and elimination of organophosphorus pesticides (OPPs) in two kinds of edible plants, cabbage (Brassica parachinensis) and aloe (Barbadensis). The metabolism of fenthion in aloe was also investigated by the liquid chromatography tandem mass spectrometry analysis (LC–MS/MS) to understand the fate of OPPs in living plants better. Transpiration stream concentration factor (TSCF) and depuration rate constants of the OPPs in living plants were obtained therein. The health risk of the OPPs treated aloe was estimated by the maximum residue limit (MRL) approach, and it revealed that the OPPs were rather safe for their fast degradable property. However, peak concentration of fenthion-sulfoxide was found to exceed the MRL and was higher than that of the parent fenthion, which indicated the potential risk of pesticide metabolites. This study highlighted the application of in vivo SPME for contaminant tracing in different living edible plants. The in vivo tracing method is very convenient and can provide more data to evaluate the risk of different pesticides, which are very important for the safety of agriculture production. - Highlights: • In vivo SPME was employed to sample organophosphorus pesticides in vegetables. • Uptake and elimination of OPPs were traced in cabbage and aloe. • In vivo tracing of fenthion demonstrated its metabolites could be rather dangerous. • The risks of OPPs were assessed based on the in vivo tracing data.

Determinación de sexo en restos prehistóricos aislados del este uruguayo

Directory of Open Access Journals (Sweden)

Figueiro, Gonzalo

2001-01-01

Full Text Available El frecuente hallazgo de restos humanos desarticulados y dispersos en sitios de la región Este del territorio uruguayo da lugar a la necesidad de recurrir en muchos casos a métodos alternativos de determinación de sexo, basados en análisis discriminantes. Las muestras de las que se parte en estos análisis son ya de poblaciones actuales, ya de poblaciones prehistóricas distintas a las representadas en el registro arqueológico uruguayo. Al determinar el número mínimo de individuos de un conjunto disperso, la edad y el sexo de los individuos representados cobran gran utilidad, y la precisión de los métodos de determinación de sexo basados en piezas aisladas se vuelve crucial. Este trabajo presenta la evaluación de 24 ecuaciones discriminantes canónicas sobre medidas de 7 huesos, en una muestra arqueológica de 15 individuos de dos sitios con estructuras monticulares ("cerritos de indios" del Este Uruguayo. El sexo de los esqueletos fue determinado anteriormente mediante métodos basados en la pelvis, el cráneo, o ambos. Los métodos evaluados presentaron grados de exactitud general de entre el 30 y el 100 por ciento, sin una dirección particular en los errores de clasificación. Esto pone de relieve la necesidad de revisar las ecuaciones utilizadas, recurriendo ya sea a una reelaboración de los coeficientes utilizados, o a un desplazamiento de los puntos de corte.

Microwave-Assisted Dip Coating of Aloe Vera on Metallocene Polyethylene Incorporated with Nano-Rods of Hydroxyapaptite for Bone Tissue Engineering

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Hairong Wang

2017-10-01

Full Text Available Bone tissue engineering widely explores the use of ceramic reinforced polymer-matrix composites. Among the various widely-used ceramic reinforcements, hydroxyapatite is an undisputed choice due to its inherent osteoconductive nature. In this study, a novel nanocomposite comprising metallocene polyethylene (mPE incorporated with nano-hydroxyapaptite nanorods (mPE-nHA was synthesized and dip coated with Aloe vera after subjecting it to microwave treatment. The samples were characterized using contact angle, Fourier transform infrared spectroscopy (FTIR, scanning electron microscope (SEM, atomic force microscopy (AFM and 3D Hirox microscopy scanning. Contact angle results show that the hydrophilicity of mPE-nHA improved notably with the coating of Aloe vera. The surface topology and increase in surface roughness were observed using the SEM, AFM and 3D Hirox microscopy. Blood compatibility assays of pure mPE and the Aloe vera coated nanocomposite were performed. The prothrombin time (PT was delayed by 1.06% for 24 h Aloe-vera-treated mPE-nHA compared to the pristine mPE-nHA. Similarly, the 24 h Aloe-vera-coated mPE-nHA nanocomposite prolonged the activated partial thromboplastin time (APTT by 41 s against the control of pristine mPE-nHA. The hemolysis percentage was also found to be the least for the 24 h Aloe-vera-treated mPE-nHA which was only 0.2449% compared to the pristine mPE-nHA, which was 2.188%. To conclude, this novel hydroxyapatite-reinforced, Aloe-vera-coated mPE with a better mechanical and anti-thrombogenic nature may hold a great potential to be exploited for bone tissue engineering applications.

Prevention of radiation and cadmium induced haematological alternations in the Swiss albino mice by Aloe Vera

International Nuclear Information System (INIS)

Agarwal, Manisha; Songara, Venkteshwar; Singariya, Seema; Meena, Dinesh; Chakrawarti, Aruna; Purohit, R.K.

2013-01-01

The development of effective radio protectors and radio recovery drugs is of great importance in view of their potential application during both planned (i.e., radiotherapy) and unplanned radiation exposure (i.e., in the nuclear industry and natural background radiation). The combined effect of radiation and cadmium further increases the causation of damages to organs and tissues. Aloe vera has enjoyed a reputation as a healer for millennia, based primarily on anecdotal evidence. For the last 40 years concerted efforts by the scientific research community has brought Aloe vera out of the realm of folk medicine, providing it solid medical and scientific foundation. Haematopoietic organs are among the most radiosensitive cells in the living organisms. Therefore, present study was carried out to study the modulatory influence of Aloe vera against radiation and cadmium induced hematological changes in the Swiss albino mice. For the study, six to eight weeks old male Swiss albino mice were procured and kept in polypropylene cages.The animals were exposed 3.5 Gy and 7.0 Gy of gamma radiation with or without cadmium chloride treatment. The Aloe vera was administered seven days prior to irradiation or cadmium chloride treatment. Five animals from each group were autopsied by cervical dislocation at each post treatment interval of 1, 2, 4, 7, 14 and 28 days. Blood was collected in heparinized tubes to estimate various haematological parameters viz. RBC, WBC, PCV, Haemoglobin and MCV. Radiation exposure resulted in a significant decline in RBC, WBC, PCV, Haemoglobin and MCV up to day-14 in peripheral blood, thereafter it increased up to day-28 without reaching to normal. After combined treatment of radiation and cadmium chloride the more severe changes were noticed showing synergistic or additive effect. An early and fast recovery was seen in Aloe vera pretreatment groups. Thus, it may be concluded from above observation that Aloe vera has the potential of combating the

Extracts from Aloe ferox and Withania somnifera inhibit Candida ...

African Journals Online (AJOL)

SERVER

2008-01-04

Jan 4, 2008 ... Herbal medicines play a vital role in the treatment of sexually transmitted infections (STIs), especially in the remote areas of South Africa where clinics and hospitals are sparsely located. Aloe ferox and. Withania somnifera are among the southern African plants commonly used for the treatment of (STIs).

In vitro activity of Aloe vera inner gel against Helicobacter pylori strains.

Science.gov (United States)

Cellini, L; Di Bartolomeo, S; Di Campli, E; Genovese, S; Locatelli, M; Di Giulio, M

2014-07-01

Aloe barbadensis Miller (Aloe vera) is a herbal remedy widely used for a variety of illnesses; A. vera leaf extracts have been promoted for detoxification, cure constipation, help flush out toxins and wastes from the body, promote digestion and are used in the treatment of peptic ulcer for cytoprotective action. The aim of this study was to evaluate the antibacterial activity of A. vera inner gel against both susceptible and resistant Helicobacter pylori strains isolated in Abruzzo region, Italy. The inner gel of leaves of a 5-year-old plant of A. vera was extracted, homogenized and tested from 800 to 1.56 mg ml(-1) against 14 clinical strains and one reference strain of H. pylori using the broth microdilution methodology. Furthermore, the sample of A. vera was investigated for the chemical fingerprint of anthraquinones. The inhibitory concentrations of A. vera inner gel were similar to the bactericidal ones, with values ranging from 6.25 to 800 mg ml(-1) . Fifty per cent of the detected strains, independently of their susceptibility profile, were inhibited in their growth at 100 mg ml(-1) . Aloe vera inner gel expresses antibacterial properties against H. pylori and, therefore, in combination with antibiotics, could represent a novel strategy for the treatment of the infection of H. pylori, especially in cases of multiresistance. The study demonstrates that the Aloe vera inner gel expresses antibacterial properties against both susceptible and resistant Helicobacter pylori strains. These findings may impact on the antimicrobial resistance phenomenon of H. pylori, proposing the A. vera inner gel as a novel effective natural agent for combination with antibiotics for the treatment of H. pylori gastric infection. © 2014 The Society for Applied Microbiology.

Protective efficacy of Aloe vera against radiation and cadmium induced haematological changes in the Swiss Albino mice

International Nuclear Information System (INIS)

Agarwal, Manisha; Purohit, R.K.; Chakrawarti, Aruna; Basu, Arindam; Bhartiya, K.M.

2011-01-01

The aim of the present study was to evaluate the protective effect of Aloe vera against radiation and cadmium induced haematological changes in the Swiss albino mice; 6-8 weeks old animals from each of the experimental groups were sacrificed by cervical dislocation at each post treatment intervals of 1,2,4,7,14 and 28 day. After sacrificing the animals, the blood was collected by cardiac puncture in heparinized tubes for various haematological studies. The values of RBC, WBC, Haemoglobin and PCV were found to decrease up to day-14 in non drug treated groups (II,III and IV), thereafter they increased on day-28. Whereas the values decreased upto day-7 in Aloe vera treated groups (V,VI,VlI) thereafter increased tip to day-28. On the other hand, the value of MCV increased upto day- 14 in non-drug treated groups (II, III, IV) and tip to day-7 in drug treated groups (V, VI, VII), thereafter it decreased tip to day-28. After combined treatment of radiation and cadmium chloride synergistic effects were observed. The Aloe vera treated animals exhibited less severe damage as compared to non-drug treated animals at all the corresponding intervals. An early and fast recovery was noticed in Aloe vera pretreated animals. Thus, it appears that Aloe vera is potent enough to check cadmium and radiation induced haematological changes in the Swiss albino mice. (author)

Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium.

Science.gov (United States)

Saniasiaya, Jeyasakthy; Salim, Rosdan; Mohamad, Irfan; Harun, Azian

2017-01-01

Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian Aloe vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans. This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC). For A. niger, a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts of Aloe vera leaf for C. albicans . The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone of inhibition was obtained for C. albicans the MIC was not determined. The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the aqueous extract for A. niger ( p Malaysian Aloe vera has a significant antifungal effect towards A. niger.

Successful Treatment of Acute Radiation Proctitis with Aloe Vera: A Preliminary Randomized Controlled Clinical Trial.

Science.gov (United States)

Sahebnasagh, Adeleh; Ghasemi, Arash; Akbari, Jafar; Alipour, Abbas; Lashkardoost, Hossein; Ala, Shahram; Salehifar, Ebrahim

2017-11-01

Acute radiation proctitis (ARP) is a common side-effect that affects up to 50% of patients receiving radiotherapy. The aim of this study was to evaluate the role of a topical preparation of Aloe vera in the treatment of ARP induced by radiotherapy of pelvic area. In this double-blind placebo-controlled trial, 20 consecutive patients with ARP after external-beam radiation therapy (46-72 Gy) of pelvic malignancies were randomized to receive either Aloe vera 3% or placebo ointment, 1 g twice daily for 4 weeks. These patients presented with at least two of the following symptoms: rectal bleeding, abdominal/rectal pain, diarrhea, or fecal urgency. These symptoms were rated by the patients in terms of their severity (grade 0-4) for each of the symptoms mentioned earlier at baseline and then weekly for 4 weeks. A symptom index was calculated by the addition of the scores (16 most symptomatic). Radiation Therapy Oncology Group (RTOG) acute toxicity criteria and psychosocial status of the patients were also recorded weekly. The lifestyle impact of the symptoms was assessed by questionnaire grading from 0 (no effect on daily activity) to 4 (afraid to leave home). There was a significant (p Aloe vera) for diarrhea (median score: 0.67 vs. 0.11), fecal urgency (median score: 0.89 vs. 0.11), clinical presentation total (median score: 4.33 vs. 1.22), RTOG total (median score: 2.89 vs. 0.89), and lifestyle (median score: 1.1 vs. 0.33). Hemorrhage and abdominal/rectal pain did not improve significantly. The odds ratios for advantage of Aloe vera over placebo for "clinical presentation total" and "RTOG total" were 3.97 (1.3-11.9) and 5.9 (1.6-21.6), respectively. A substantial number of patients with radiation proctitis seem to benefit from therapy with Aloe vera 3% ointment.

Chemical composition of volatile constituents from the leaves of Aloe ...

African Journals Online (AJOL)

AJB SERVER

2006-09-18

Sep 18, 2006 ... very helpful enzymes, saponins, hormones and amino acids which can be absorbed into the human skin. One of the constituents of the Aloe pith is ... higher than green tea and grape seed extracts, respectively. It is also well ...

Presentación del Libro “Tres Médicos Árabes” Rhases, Averroes, Avicena

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Efraim Otero Ruiz

2007-12-01

Pero esa misma migratoriedad, especialmente de las del norte, hizo que gradualmente se extendieran por Siria, Mesopotamia y por todo el antiguo imperio Persa hasta la India, por el oriente; y por Egipto y la costa norte del Africa hasta Gibraltar, por el occidente. Unidos por lenguajes ancestrales derivados del sumerio, el babilonio, el sánscrito y el urdu, que dieron origen al idioma árabe, en esas regiones había enclaves de muchas tribus y muchas religiones, mono y politeístas. El genio de Mahoma y su Corán fue el unificarlos monoteísticamente y darles un sentido de trascendencia, en esta vida y en la otra. Por eso, en el milenio que nos ocupa, se cumplió lo que después describiría maravillosamente Carlyle, el pensador inglés: Aquella estirpe, polvo inflamable por mucho tiempo aislado en el desierto, de golpe prendió fuego y sus llamas formidables se elevaron al cielo desde Dehli, en la India, hasta Granada en España...

Protective role of aloe vera against radiation and cadmium induced biochemical changes in the jejunum of Swiss albino mice

International Nuclear Information System (INIS)

Purohit, R.K.; Bhartiya, K.M.; Agarwal, Manisha; Swami, Rajesh; Chakrawarti, Aruna; Meena, Dharmpal

2007-01-01

Full text: The extensive use of atomic energy now a days in various branches of natural economy, science and technology, radio diagnosis, radiotherapy, industries, agriculture, nuclear research etc. has made radiation injury an urgent problem attracting the attention not only of specialists in a variety of clinical disciplines but also of a vast army of theoretical scientists. Metals like cadmium have always been intrinsic components of earth crust with the continuing trends towards and increasing human activities involving man may become exposed to concentration of toxic metals presenting a potential threat for survival. The severity of the damage can be modulated by treating the animals with antioxidants. In view of the potential for practical application, a variety of compounds are being tested for their radio protective activities. Among these, Aloe vera hold a great promise. Aloe vera juice was obtained from Millennium Agro Company, Goregaon (W) Mumbai. It is a herbal drug and known to contain well over 100 separate ingredients or constituents between those found in the leaf and mucilaginous gel inside the leaf. In light of the above, the present study was aimed to evaluate the protective effect of Aloe vera against radiation and cadmium induced biochemical changes in the jejunum Swiss albino mice. For this purpose, healthy adult male Swiss albino mice were divided into seven groups. Group I included sham-irradiated normal mice. Group II was administered CdCl 2 at the dose of 20ppm, while Group III was exposed to 5.0 Gy of gamma radiation. Animals of Group IV were treated with both CdCl 2 and 5.0 Gy of gamma rays. The animals of Group V and VI were treated with CdCl 2 + Aloe vera and 5.0Gy + Aloe vera respectively, whereas Group VII was treated with CdCl 2 +5.0Gy+ Aloe vera. In the groups V, VI and VII the Aloe vera was given seven days prior to the treatment of CdCl 2 or gamma rays. Three animals from all the experimental groups were sacrificed by cervical

Del pergamino al bit. La influencia del soporte en el contenido

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Nistal, Ana

2009-06-01

Full Text Available The printed book has been one of the most revolutionary developments in European history; the coming of the book was possible tanks to the development of the printing industry. The evolution of the new technology around the book industry caused profound changes affecting authors, producers and readers. New medium favoured new ways to create contents. Digital technology is pushing another revolution that threatens to change the world of the book, not only in terms of physical media, but also the concept of isolated book, closed by cardboard covers, with delimited beginning and end. The big change is not the transition from printed characters to bits; the change will be the transition from isolated books to connected books, which can be accessed trough multiples ways (or “links”, open books against to the closed books of the Gutenberg age.El libro moderno de papel ha sido, sin duda, uno de los instrumentos más revolucionarios en la historia europea; su aparición fue posible gracias a las nuevas tecnologías de impresión y edición en papel. El cambio que supuso el desarrollo industrial de los libros cambió totalmente la concepción de los creadores, distribuidores y lectores. Los nuevos y modernos soportes propiciaron nuevas formas para crear contenidos antes imposibles. La tecnología digital está propiciando otra gran revolución que amenaza con cambiar el mundo del libro, no sólo en cuanto al soporte físico, sino a su concepto como libro aislado, delimitado por unas tapas de cartón, con un principio y un fin concretos. El gran cambio no es el paso de letras impresas a bits, sino el paso de libros aislados a libros “conectados”, a los que se puede acceder mediante infinitas fórmulas (o “conexiones” en oposición a los libros cerrados de la era Gutenberg.

EVALUATION OF FREEZE DRIED ALOE VERA AND NOPAL CACTUS FOR POSSIBLE HEALTH TREATMENTS BY COMPARISON OF ANTIOXIDANT PROPERTIES AND FREE RADICAL INHIBITION.

Science.gov (United States)

Rodriguez, Kenneth R; Jones, Anthony E; Belmont, Barbara

2014-01-01

The goal of this project was to characterize the antioxidant powers of lyophilized Aloe Vera ( Aloe barbadensis ) and Nopal Cactus (Opuntia ficus-indica) by quantifying the phenolics content and radical scavenging abilities of preparations derived from these plants. Extracts of these lyophylized succulents were assayed for phenolic compounds by the Folin Ciocalteau method and compared for free radical scavenging capability by the DPPH method. We found that even though the Aloe lyophilizate extract contained more phenolic content, the Nopal lyophilizate exhibited better free radical scavenging ability. Aloe Vera extract contained 0.278 g/L of phenolic content and exhibited 11.1% free radical inhibition, with a free radical scavenging rate constant of 0.177±0.015 min -1 . Nopal Cactus extract contained 0.174 g/L of phenolic content and exhibited 13.2% free radical inhibition, with a free radical scavenging rate constant of 0.155±0.009 min -1 . These results showed Nopal to have greater antioxidant potency than Aloe.

La escritura académica como proceso epistémico en la enseñanza del derecho penal

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Caviedes Estanislao Escalante-Barreto

2015-05-01

Full Text Available La superación de concepciones pedagógicas basadas en modelos conductistas y la implementación de otras formas metodológicas pueden llevar al "aprendizaje crítico natural". Este es un escenario propicio para generar nuevas formas de construir conocimiento, considerando a los partícipes del acto educativo como sujetos de interacción pedagógica. Este contexto permite identificar la escritura y la alfabetización académica como procesos epistemológicos de construcción y transformación del conocimiento penal. Por lo que se plantea como reto la necesidad de reconocer que la escritura es un proceso que se construye de manera permanente por comunidades académicas y no por individuos aislados. Este proceso se caracteriza por permitir la construcción de conocimiento nuevo, reflexivo y analítico en el aprendizaje/enseñanza, el cual debe ser reconocido como parte del proceso pedagógico y de reflexión sobre el acto educativo para transformar la enseñanza del derecho penal.

Co-expression of NCED and ALO improves vitamin C level and tolerance to drought and chilling in transgenic tobacco and stylo plants.

Science.gov (United States)

Bao, Gegen; Zhuo, Chunliu; Qian, Chunmei; Xiao, Ting; Guo, Zhenfei; Lu, Shaoyun

2016-01-01

Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosanthes guianensis (Aublet) Swartz], a forage legume, plants co-expressing stylo 9-cis-epoxycarotenoid dioxygenase (SgNCED1) and yeast D-arabinono-1,4-lactone oxidase (ALO) genes were generated in this study, and tolerance to drought and chilling was analysed in comparison with transgenic tobacco overexpressing SgNCED1 or ALO and the wild-type plants. Compared to the SgNCED1 or ALO transgenic plants, in which only ABA or AsA levels were increased, both ABA and AsA levels were increased in transgenic tobacco and stylo plants co-expressing SgNCED1 and ALO genes. Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants, while an enhanced chilling tolerance was observed in ALO transgenic tobaccos with induced expression of cold-responsive genes, but not in SgNCED1 plants. Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic tobacco and stylo plants with induced expression of both drought and cold-responsive genes. Our result suggests that co-expression of SgNCED1 and ALO genes is an effective way for use in forage plant improvement for increased tolerance to drought and chilling and nutrition quality. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

Radiometric, geometric, and image quality assessment of ALOS AVNIR-2 and PRISM sensors

Science.gov (United States)

Saunier, S.; Goryl, P.; Chander, G.; Santer, R.; Bouvet, M.; Collet, B.; Mambimba, A.; Kocaman, Aksakal S.

2010-01-01

The Advanced Land Observing Satellite (ALOS) was launched on January 24, 2006, by a Japan Aerospace Exploration Agency (JAXA) H-IIA launcher. It carries three remote-sensing sensors: 1) the Advanced Visible and Near-Infrared Radiometer type 2 (AVNIR-2); 2) the Panchromatic Remote-Sensing Instrument for Stereo Mapping (PRISM); and 3) the Phased-Array type L-band Synthetic Aperture Radar (PALSAR). Within the framework of ALOS Data European Node, as part of the European Space Agency (ESA), the European Space Research Institute worked alongside JAXA to provide contributions to the ALOS commissioning phase plan. This paper summarizes the strategy that was adopted by ESA to define and implement a data verification plan for missions operated by external agencies; these missions are classified by the ESA as third-party missions. The ESA was supported in the design and execution of this plan by GAEL Consultant. The verification of ALOS optical data from PRISM and AVNIR-2 sensors was initiated 4 months after satellite launch, and a team of principal investigators assembled to provide technical expertise. This paper includes a description of the verification plan and summarizes the methodologies that were used for radiometric, geometric, and image quality assessment. The successful completion of the commissioning phase has led to the sensors being declared fit for operations. The consolidated measurements indicate that the radiometric calibration of the AVNIR-2 sensor is stable and agrees with the Landsat-7 Enhanced Thematic Mapper Plus and the Envisat MEdium-Resolution Imaging Spectrometer calibration. The geometrical accuracy of PRISM and AVNIR-2 products improved significantly and remains under control. The PRISM modulation transfer function is monitored for improved characterization.

BIOLOGICAL CLONING OF A BOVINE CORONAVIRUS ISOLATE

OpenAIRE

Betancourt, A; Rodríguez, Edisleidy; Relova, Damarys; Barrera, Maritza

2008-01-01

Con el objetivo de obtener un aislado de Coronavirus bovino clonado biológicamente se adaptó el aislado VB73/04 a la multiplicación en la línea celular MDBK. Este aislado indujo la formación de placas, las cuales resultaron homogéneas después del clonaje biológico. La población viral obtenida fue identificada como Coronavirus bovino por RT-PCR y Seroneutralización. In order to obtain a biologically cloned bovine coronavirus isolate, the isolate VB73/04 was adapted to multiplication in MDBK...

Actividad molusquicida del Paraiso (Melia azedarach L. (Meliaceae sobre Lymnaea cubensis, molusco vector de Fasciolosis

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Perez Marlene Piña

1998-01-01

Full Text Available INTRODUCCIÓN: La Fasciolosis constituye en diversas regiones una fuente importante de pérdidas económicas y cuando se descuidan las medidas de control de sus hospedadores (moluscos, junto a condiciones ecológicas favorables, pueden ocurrir casos aislados de Fasciolosis humana. Dentro de los métodos alternativos para su control está el uso de extractos vegetales y se pretendió evaluar el probable empleo del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. en el control de Lymnaea cubensis, principal vector de la Fasciolosis en Cuba. MATERIAL Y MÉTODO: Diferentes concentraciones del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. fueron testados para determinar las dosis letales media (DL50 y máxima (DL90 usando un programa computarizado Probit-Log. Siete series experimentales fueron probadas usando 72 moluscos en cada una. Para determinar la influencia sobre la frecuencia cardíaca fueron testados tres grupos de 10 moluscos, dos fueron tratados con las DL50=0,88627 y DL90=1,7641, respectivamente, mientras que el tercero fue considerado como testigo. RESULTADOS Y CONCLUSIONES: Se observó una marcada influencia de ambas dosis sobre la frecuencia cardíaca del molusco estudiado. Estos resultados son alentadores, pues demuestran el potencial empleo de esta planta en el control de moluscos indeseables.

Actividad molusquicida del Paraiso (Melia azedarach L. (Meliaceae sobre Lymnaea cubensis, molusco vector de Fasciolosis

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Marlene Piña Perez

1998-06-01

Full Text Available INTRODUCCIÓN: La Fasciolosis constituye en diversas regiones una fuente importante de pérdidas económicas y cuando se descuidan las medidas de control de sus hospedadores (moluscos, junto a condiciones ecológicas favorables, pueden ocurrir casos aislados de Fasciolosis humana. Dentro de los métodos alternativos para su control está el uso de extractos vegetales y se pretendió evaluar el probable empleo del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. en el control de Lymnaea cubensis, principal vector de la Fasciolosis en Cuba. MATERIAL Y MÉTODO: Diferentes concentraciones del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. fueron testados para determinar las dosis letales media (DL50 y máxima (DL90 usando un programa computarizado Probit-Log. Siete series experimentales fueron probadas usando 72 moluscos en cada una. Para determinar la influencia sobre la frecuencia cardíaca fueron testados tres grupos de 10 moluscos, dos fueron tratados con las DL50=0,88627 y DL90=1,7641, respectivamente, mientras que el tercero fue considerado como testigo. RESULTADOS Y CONCLUSIONES: Se observó una marcada influencia de ambas dosis sobre la frecuencia cardíaca del molusco estudiado. Estos resultados son alentadores, pues demuestran el potencial empleo de esta planta en el control de moluscos indeseables.

Enhanced biocompatibility of ZnS:Mn quantum dots encapsulated with Aloe vera extract for therapeutic applications

Science.gov (United States)

Anilkumar, M.; Bindu, K. R.; Sneha Saj, A.; Anila, E. I.

2016-08-01

Toxicity of nanoparticles remains to be a major issue in their application to the biomedical field. Aloe vera (AV) is one of the most widely exploited medicinal plants that have a multitude of amazing properties in the field of medicine. Methanol extract of Aloe vera can be used as a novel stabilising agent for quantum dots to reduce toxicity. We report the synthesis, structural characterization, antibacterial activity and cytotoxicity studies of ZnS:Mn quantum dots synthesized by the colloidal precipitation method, using methanol extract of Aloe vera (AVME) as the capping agent. The ZnS:Mn quantum dots capped with AVME exhibit superior performances in biocompatibility and antibacterial activity compared with ZnS:Mn quantum dots without encapsulation. Project supported by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India.

Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

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Yun Sun Lee

Full Text Available Aloe vera (Asphodeloideae is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

Science.gov (United States)

Lee, Yun Sun; Ju, Hyun Kyoung; Kim, Yeon Jeong; Lim, Tae-Gyu; Uddin, Md Romij; Kim, Yeon Bok; Baek, Jin Hong; Kwon, Sung Won; Lee, Ki Won; Seo, Hak Soo; Park, Sang Un; Yang, Tae-Jin

2013-01-01

Aloe vera (Asphodeloideae) is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

Mitigation by Aloe Vera of cadmium chloride and radiation induced biochemical changes in the brain of Swiss albino mice

International Nuclear Information System (INIS)

Chakrawarti, Aruna; Kanwar, Om; Nayak, Kamal Kumar; Ranga, Deepti; Jangir, Ashok; Ram, Purkha

2013-01-01

Whole body exposure to ionizing radiation provokes oxidative damage, organ dysfunction and metabolic disturbance. Herbal drugs offer an alternative to the synthetic radioprotective compounds which are either non-toxic or less toxic. Aloe vera rich in polyphenolic compound is known to possess antioxidant properties. In the context, the present study, effect of Aloe vera against radiation and cadmium induced changes in the brain of Swiss albino mice. For the purpose, six to eight weeks old male Swiss albino mice were selected and divided into seven groups:- Group I (Sham-irradiated), Group II (treated with cadmium chloride 20 ppm), Group III (Irradiated with 7.0 Gy gamma rays), Group IV (Both irradiated and treated with cadmium chloride solution), Group V (Cadmium and Aloe vera treated), Group VI (radiation and Aloe vera treated), Group VII (radiation, and cadmium chloride and Aloe vera treated). The animals were sacrificed by cervical dislocation at each post-treatment intervals of 1, 2, 4, 7, 14 and 28 days. The brain (cerebral cortex) was taken out and quantitatively analyzed for different biochemical parameters such as total proteins, glycogen, cholesterol, acid phosphatase activity, alkaline phosphatase activity, DNA and RNA. The value of cholesterol, glycogen, RNA, acid phosphatase activity, and alkaline phosphatase activity increased up to day-14 in non drug-treated groups and day-7 in Aloe vera treated groups and thereafter decreased up to day-28. The value of total proteins and DNA decreased up to day-14 in non drug-treated groups and day-7 in the drug treated groups then increased in all groups. In only cadmium chloride (Without and with drug) treated animals (Groups II and V) the value of cholesterol decreased during early intervals (days-14 and 7 respectively) and increased thereafter. Severe changes were observed after combined exposure to radiation and cadmium chloride showing synergistic effect. Aloe vera reduced the severity of damage and made the

Preparation and characterization of novel wound dressing based on silver nanoparticle-impregnated bacterial cellulose and bacterial cellulose-aloe vera

International Nuclear Information System (INIS)

Ventigan, Sarla V.; Santiago, Karen S.; Balitaan, Jolleen Natalie I.

2015-01-01

Ideal wound dressings stimulate wound healing, control unpleasant odors, and provide antimicrobial action in wounds. However, most traditional wound dressings such as gauze and biological dressings exhibit exudate leaking which increases the risk of infection and delayed wound healing of tissues. This study aims to develop and characterize a bio-composite of bacterial cellulose and aloe vera having the ideal features of a wound dressing from Acetobacter xylinum-activated culture medium supplemented with various aloe vera concentrations from )-50% (v/v) and the film which exhibits the most uniform results is used for the incorporation of silver nanoparticle as an antibacterial agent. The biopolymer composites of bacterial cellulose and aloe vera were developed by adding 0-50% aloe vera (v/v) in the A. xylinum-activated coconut water medium during biosynthesis in static cultivation for 10 days. The films obtained after drying the membranes were named as bacterial cellulose-aloe vera (BC-A) films. The moisture content of films reached 99% which indicates that the films may be suitable for providing a moist environment to facilitate wound healing fast. With the addition of aloe vera up to 30% (v/v) during BC synthesis, it resulted in a significant improvement in the water absorption capacity of the films showing a WAC ration of 36.46 (r.s.d.= 12.17%, n=3) compared to the unmodified film having a ratio of 9.03 (r.s.d.= 13.95%, n=3). However, the addition of aloe vera at a concentration greater than 30% (v/v) resulted in a decrease in pellicle formation which can be observed from the very weak properties of the films. The BC-A (30%) displayed significantly improved in comparison to the unmodified BC film. Also, it is capable of absorbing high amount of water than its weight and can act as a potential wound dressing which reduces irritation and inflammation. (author)

Lack of Prophylactic Effects of Aloe Vera Gel on Radiation Induced Dermatitis in Breast Cancer Patients

OpenAIRE

Ahmadloo, Niloofar; Kadkhodaei, Behnam; Omidvari, Shapour; Mosalaei, Ahmad; Ansari, Mansour; Nasrollahi, Hamid; Hamedi, Sayed Hasan; Mohammadianpanah, Mohammad

2017-01-01

Purpose: Radiotherapy induced dermatitis is a common and sometimes serious side effect. We conducted a randomized study to understand whether the adjunctive use of aloe vera gel might reduce the prevalence and/or severity of radiotherapy induced dermatitis. Methods: One hundred patients with newly diagnosed breast cancer were randomized to receive aloe vera gel or nothing during adjuvant radiotherapy consisting of conventional external beam radiation using 6 MV mega voltage linear accelerator...

Proyecto regenerativo del campus: aprendiendo a través de las dinámicas de la naturaleza y la universidad

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José Ripper Kos

2017-12-01

Full Text Available La sustentabilidad del planeta exige un cambio significativo en la forma cómo nos relacionamos con el medio ambiente. En ese contexto, el gran desafío consiste en pasar del discurso a la práctica. Las universidades, como formadoras de futuros profesionales e investigadores, deberían asumir una posición de liderazgo en este proceso. Sin embargo, la fragmentación del conocimiento, en áreas o departamentos aislados, no contribuye a la implementación de esos cambios relevantes. El proceso de desarrollo de un proyecto regenerativo para el campus de la Universidad Federal de Santa Catarina, en Brasil, constituye una propuesta para el debate sobre el papel del espacio físico universitario como instrumento pedagógico para la resiliencia de los ecosistemas. El concepto del proyecto surge del análisis y las proposiciones de estudiantes de posgrado, provenientes de diferentes áreas formativas, las cuales se orientan a desarrollar propuestas claras en las que dinámicas y ciclos de la naturaleza puedan aportar positivamente a la calidad de aprendizaje y viceversa

Fumaric acid, an antibacterial component of Aloe vera L. | He ...

African Journals Online (AJOL)

The acetoacetate fraction partitioned from Aloe vera L. showed an effective antibacterial activity against four clinically frequent pathogenic bacteria. Through a series of chromatographic methods and activity assays, one compound was obtained and it has potent antibacterial activity. Based on the data of mass spectrometry, ...

Antioxidant activity of the exudate from Aloe barbadensis leaves in ...

African Journals Online (AJOL)

The effects of the exudate of Aloe barbadensis leaves on oxidative stress and some antioxidant status of streptozotocin induced - diabetic rats were studied. There was significant reduction in scavenging enzymes like superoxide dismutase (SOD) activity and significant increase in signs of oxidative tissue damage, such as ...

Prevention of the onset of hyperglycaemia by extracts of Aloe ...

African Journals Online (AJOL)

GREGO

of Aloe barbadensis in rabbits treated with alloxan. Afolabi C. Akinmoladun1* and Oluyemi Akinloye2. 1Department of Biochemistry, Federal University of Technology, Akure, Nigeria. ... In this study, the inhibitory effects of a home-made ... ascorbic acid, citric acid, potassium sorbate, sodium benzoate, ... cholesterol diet.

Processing and stabilization of Aloe Vera leaf gel by adding chemical and natural preservatives

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N. Nazemi

2017-11-01

Full Text Available Background and objectives: Aloe vera has been used as a medicinal herb for thousands of years. Aloe vera leaves can be separated into latex and gel which have biological effects. Aloe gel is a potent source of polysaccharides. When the gel is exposed to air, it quickly decomposes and decays and loses most of its biological activity. There are various processing techniques for sterilizing and stabilizing the gel. The aim of this study was to improve stabilization of the gel by adding some chemical and natural preservatives. Methods: The gel was obtained from Aloe vera leaves and after some processing chemical and natural preservatives were added. Chemicals included citric acid, ascorbic acid, vitamin E and potassium sorbate while natural preservatives were two essential oils derived from Cinnamomum zeylanicum and Eugenia caryophyllata. All these operations were performed under sterile conditions and they were evaluated at different temperatures and times. Appearance and taste changes of gel were studied organoleptic. Microbiological tests and some physical assays such as pH, refractometry and viscosity properties as well as determination of total sugars were measured. NMR and FT-IR analyses were performed for determining the quality of samples. Results: After data analyzing, the results showed that the samples formulated with chemical additives together with essential oils were more suitable and stable compared to the control samples after 90 days and the effective ingredient acemannan, remained stable. Conclusion: The stable gel can be considered for therapeutic properties and be used for edible and medicinal purposes.

Detection of seagrass beds in Khunk Graben Bay, Thailand, using ALOS AVNI2 image

Science.gov (United States)

Komatsu, Teruhisa; Noiraksar, Thidarat; Sakamoto, Shingo X.; Sawayama, Shuhei; Miyamoto, Hiroomi; Phauk, Sophany; Thongdee, Pornthep; Jualaong, Suthep; Nishida, Shuhei

2012-11-01

Coastal habitats having high productivity provide numerous ecological services such as foods, protection from strong waves through buffering effect, fixation of CO2 through photosynthesis, fostering biodiversity etc. However, increasing human impacts and climate change decrease or degrade coastal habitats. ASEAN region is developing most rapidly in the world. In the developing region, it is necessary to grasp present spatial distributions of habitats as a baseline data with standardized mapping methods. Remote sensing is one of the most effective methods for mapping. Japan Aerospace Exploration Agency (JAXA) provides non-commercial satellite images with ultra-high spatial resolution optical sensors (10 m), AVNIR2, similar to LANDSAT TM. Using ALOS AVNIR2 images it may be possible to make habitat map in the region. In Thailand, shrimp ponds cause degradation of coastal ecosystem through cutting mangroves and eutrophicated discharge from ponds. We examined capability of remote sesing with ALOS AVNIR2 to map seagrass beds in Khung Kraben Bay, Chanthaburi Province, Thailand, surrounded by shrimp ponds. We analyzed ALOS AVNIR2 taken on 25 January 2008. Ground truth survey was conducted in October 2010 using side scan sonar and scuba diving. The survey revealed that there were broad seagrass beds consisting of Enhalus acroides. We used a decision tree to detect seagrass beds in the bay with quite turbid seawater coupled with Depth-Invariant Index proposed by Lyzenga (1985) and bottom reflectances. We could succeed to detect seagrass beds. Thus it is concluded that ALOS AVNIR2 is practical to map seagrass beds in this region.

Aloe vera: Potential candidate in health management via modulation of biological activities

Science.gov (United States)

Rahmani, Arshad H.; Aldebasi, Yousef H.; Srikar, Sauda; Khan, Amjad A.; Aly, Salah M.

2015-01-01

Treatment based on natural products is rapidly increasing worldwide due to the affordability and fewer side effects of such treatment. Various plants and the products derived from them are commonly used in primary health treatment, and they play a pivotal role in the treatment of diseases via modulation of biochemical and molecular pathways. Aloe vera, a succulent species, produces gel and latex, plays a therapeutic role in health management through antioxidant, antitumor, and anti-inflammatory activities, and also offers a suitable alternative approach for the treatment of various types of diseases. In this review, we summarize the possible mechanism of action and the therapeutic implications of Aloe vera in health maintenance based on its modulation of various biological activities. PMID:26392709

Assessment of Chronic Administration of Aloe Vera Gel On ...

African Journals Online (AJOL)

The study was designed to investigate the effects of chronic administration of Aloe vera gel extract on markers of hepatic damage, lipid profiles and erythrocyte osmotic fragility using the Wistar rats. Forty male Wistar rats divided into four groups of ten rats per group were used in the study. Group I which served as the control ...

Insecticidal and insect productivity reduction capacities of aloe vera ...

African Journals Online (AJOL)

Two important medicinal plants, Aloe vera and Bryophyllum pinnatum were admixed with maize grains (Popcorn) at dosages of 1g, 2g and 5g treatments per 50g of maize. Results showed that both lower and higher dosages had toxic effects on Tribolium castaneum but the best results were obtained in the grains treated ...

Influencia de la procedencia de los componentes del medio de cultivo en la calidad de los ensayos microbiológicos para la determinación de bacteriófagos

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Annia González-Crespo

2017-12-01

Full Text Available La contaminación por virus bacterianos es uno de los problemas más difíciles de evaluar en la industria biotecnológica moderna, sobre todo en las producciones de proteínas que están basadas en cepas recombinante de Escherichia coli. Para certificar que un banco de cepas está libre de bacteriófagos el método más recomendado sigue siendo la inducción de la fase del ciclo lítico sobre placas con medios semisólidos. En este trabajo nos propusimos evaluar medios de cultivos elaborados con bases de diferentes casas comerciales para la detección de bacteriófagos contaminantes. Para ello utilizamos un aislado de bacteriófago ambiental y la cepa sensible LE 392 y bases de las firmas OXOID, BioCen y Biolife. Después de estandarizar la metodología de ensayo se evaluó la habilidad de una preparación concentrada del aislado ambiental de formar placas de lisis en los medios elaborados con reactivos de las tres casas comerciales. La mayor cantidad de placas de lisis se obtuvo cuando se emplearon las bases provenientes del Centro Nacional de Biopreparados, el medio donde menos placas de lisis observamos fue empleando bases proveniente de la Casa comercial Biolife con dos órdenes de magnitud menos. El medio preparado con bases provenientes de la casa comercial OXOID fue el que mostró mejor consistencia entre lotes.

Hongos del suelo aislados de zonas de vegetación natural del páramo de Chisacá, Colombia

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Gualdrón Arenas Carmen

1997-06-01

Full Text Available Soil fungal communities from two natural paramo vegetation types, Calamagrostis effusa pajonal (grassland and Gynoxys fuliginosaforest located at 3600 m were investigated. Fungi in active mycelium stage from organic and mineral particles were obtained by the soil washing isolation technique. 1613 isolates from 600 particles, most from organic particles, were obtained. 62 species, belonging to 9 families and 36 genera, were identified. Species of Mortierella and Fusarium were dominant in grassland and forest vegetation respectively. The differences in fungal community composition are related to the vegetation and soil types.Se investigó la micoflora del suelo en dos sitios de vegetación natural localizados en el páramo de Chisacá, en vegetación de pajonales de Calamagrostis effusá a 3.600 m y en bosque de Gynoxys fuliginosa a 3.540 m. Los hongos se aislaron en estado de micelio activo, a partir de partículas orgánicas y minerales, obtenidas mediante la técnica de lavado del suelo. De 600 partículas se realizaron 1613 aislamientos, con un porcentaje de colonización de 2.68 %. Se obtuvo un total de 62 especies representados en 9 familias y 36 géneros. La mayoría de los hongos se aislaron de partículas orgánicas, aunque algunos no tienen especificidad por el tipo de sustrato soporte. Especies de Mortierella se encuentran dominantes en el sitio de Calamagrostis y especies de Fusarium en el sitio de Gynoxys. La mayor causa de variación en la composición de las comunidades microfúngicas están relacionadas con la vegetación y el tipo de suelo.

Eolic central Guerrero Negro, BCS, Mexico, performance first year operation; Central eolica Guerrero Negro, BCS, Mexico, resultados del primer ano de operacion

Energy Technology Data Exchange (ETDEWEB)

Cadenas Tovar, Roberto; Sanchez Cornejo, Carlos; Lopez Rios, Serafin [Comision Federal de Electricidad, Morelia, Michoacan (Mexico); Ley Romero, Jose R [Comision Federal de Electricidad, Guerrero Negro, Baja California Sur (Mexico)

2000-07-01

Comision Federal de Electricidad installed a 600 kW wind turbine in the Guerrero Negro isolated electrical grid to evaluate, under actual operation conditions, the contribution of wind energy in the generation of electricity. This paper describes the performance of the wind turbine in terms of its availability, power curve and electricity produced. The results have been satisfactory, electricity was supplied with a lesser fuel consumption than before. [Spanish] La Comision Federal de Electricidad instalo en Guerrero Negro, Baja California Sur, Mexico un aerogenerador de 600 kW para evaluar en condiciones reales de operacion la integracion de la energia del viento a sistemas electricos aislados basados en diesel. En este trabajo se describen los resultados del primer ano de operacion del aerogenerador en terminos de su disponibilidad, curva de potencia y generacion obtenida. Los resultados han sido satisfactorios, registrandose una reduccion en el consumo de combustible fosil en el sistema.

Efecto del Propóleos Chileno sobre el Metabolismo de Glucosa en Ratones Diabéticos

OpenAIRE

Pacheco, Alejandro; Daleprane, Julio B; Freitas, Vanessa S; Ferderbar, Simone; Hirabara, Sandro; Cuevas, Alejandro; Saavedra, Nicolás; Curi, Rui; Abdalla, Dulcineia S. P; Salazar, Luis A

2011-01-01

En el presente estudio se evaluó el efecto del propóleos sobre el metabolismo de la glucosa en ratones C57/BL-6 con diabetes mellitus tipo 2 inducida por dieta alta en grasa. Se midieron los cambios en las concentraciones séricas de lípidos, glucosa e insulina, y el efecto sobre la captación de 2-deoxi-[2,6-3H]-D-glucosa, síntesis de [14C]-glicógeno y descarboxilación de [U-14C]-D-glucosa inducida por insulina en músculo aislado. Los resultados muestran que en ratones diabéticos, el tratamien...

Aloe arborescens aqueous gel extract alters the activities of key ...

African Journals Online (AJOL)

The present study investigated the antidiabetic activity and the possible mechanisms of action of aqueous extract of Aloe arborescens leaf gel (AALGEt) on normal and alloxan-induced diabetic rats. Diabetes was induced in 12 h fasted rats by intraperitoneal injection of 140 mg/kg body weight of alloxan. Blood glucose ...

Prevention of the onset of hyperglycaemia by extracts of Aloe ...

African Journals Online (AJOL)

The ability of a home-made aqueous extract of Aloe barbadensis to prevent the onset of alloxaninduced hyperglycaemia was examined and compared with that of a factory-produced gel. Three groups of animals were administered 200 mg/kg body weight of alloxan intraperitoneally. A fourth group of animals was left ...

Utilization of plant bioactives as feed additives for poultry: The effect of Aloe vera gel and its extract on performance of broilers

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A.P Sinurat

2003-10-01

Full Text Available Feed additives are commonly added in poultry feed as a growth promotant or to improve feed efficiency. The most common feed additive used is antibiotic at sub-therapheutic doses, although there is a controversy on its impact on human health. Previous results showed that Aloe vera gel could improve feed efficiency in broilers and an in vitro study showed that the extract have an antibacterial effect. Therefore, a further experiment was designed to study the response of broilers to Aloe vera gel or its extract as feed additives. Aloe vera was prepared in dry gel or chloroform-extract and included in the diet at levels of 0.25; 0.50 and 1.00 g/kg (equal to dry gel. Standard diets with or without antibiotic were also formulated as control and a commercial diet was included for comparison. The diets were fed to broilers from day old to 5 weeks. Each treatment has 9 replicates and 6chicks/replicate. Parameters observed were: feed consumption, weight gain and feed convertion ratios. Carcass yield, abdominal fat levels, relative weight of liver, gizzard, tractus digestivus and length of tractus digestivus were also measured at the end of feeding trial. The results showed that Aloe gel and its extract did not influence body weight gain and feed consumption of broilers significantly (P>0.05, but improved feed convertion slightly (3.50%. The response in this trial was similar as thosecommercial diet and diet added with antibiotic. There was no significant (P>0.05 effect of Aloe vera bioactives on carcass yield, abdominal fat level and relative weight of liver. However, Aloe vera gel and its extract tend to increase gizzard weight, gastro intestinal weight and length. The Aloe vera gel and its extract also reduced the total count of aerobic bacteria in the digesta of tractus digestivus. It is concluded that the Aloe vera gel improve feed efficiency in broilers by increasing the size of tractus digestivus and reducing the total count of aerobic bacteria in

The potential benefits of using aloe vera in stoma patient skin care.

Science.gov (United States)

Rippon, Mark; Perrin, Angie; Darwood, Richard; Ousey, Karen

2017-03-09

Individuals living with an ostomy may suffer from a variety of peri-stomal skin complications related to the use of their stoma appliance or accessories. These conditions can be serious enough to significantly impact on a patient's quality of life and may result in severe clinical complications (such as infection). This article is a review of the literature with the objective of investigating and presenting evidence for the well-documented use of aloe vera in the prevention of skin conditions similar to those seen in peri-stomal skin complications. An exploration for the potential use of aloe vera directly or indirectly (as an adjunct to medical devices such as wafers) in stoma patients is presented with the view that this use may be beneficial in the prevention of such peri-stomal skin complications.

Evaluation of Isolated Fractions of Aloe vera Gel Materials on Indinavir Pharmacokinetics: In vitro and in vivo Studies.

Science.gov (United States)

Wallis, Lonette; Malan, Maides; Gouws, Chrisna; Steyn, Dewald; Ellis, Suria; Abay, Efrem; Wiesner, Lubbe; Otto, Daniel P; Hamman, Josias

2016-01-01

Aloe vera is a plant with a long history of traditional medicinal use and is consumed in different products, sometimes in conjunction with prescribed medicines. A. vera gel has shown the ability to modulate drug absorption in vitro. The aim of this study was to fractionate the precipitated polysaccharide component of A. vera gel based on molecular weight and to compare their interactions with indinavir pharmacokinetics. Crude polysaccharides were precipitated from a solution of A. vera gel and was fractionated by means of centrifugal filtration through membranes with different molecular weight cut-off values (i.e. 300 KDa, 100 KDa and 30 KDa). Marker molecules were quantified in the aloe leaf materials by means of nuclear magnetic resonance spectroscopy and the average molecular weight was determined by means of gel filtration chromatography linked to multi-angle-laser-light scattering and refractive index detection. The effect of the aloe leaf materials on the transepithelial electrical resistance (TEER) of Caco-2 cell monolayers as well as indinavir metabolism in LS180 cells was measured. The bioavailability of indinavir in the presence and absence of the aloe leaf materials was determined in Sprague-Dawley rats. All the aloe leaf materials investigated in this study reduced the TEER of Caco-2 cell monolayers, inhibited indinavir metabolism in LS 180 cells to different extents and changed the bioavailability parameters of indinavir in rats compared to that of indinavir alone. These indinavir pharmacokinetic modulation effects were not dependent on the presence of aloverose and also not on the average molecular weight of the isolated fractions.

Liberación de endotelina-1 por angiotensina ll en miocitos cardíacos aislados Angiotensin II-induced endothelin-1 release in cardiac myocytes

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María C. Villa-Abrille

2006-06-01

Full Text Available Muchos de los efectos de la angiotensina II (Ang II son mediados en realidad por la acción de endotelina (ET endógena liberada y/o producida en respuesta a la Ang II. En este trabajo evaluamos la interacción Ang II/ET-1, sus consecuencias en la contractilidad cardíaca y el papel de las especies reactivas del oxígeno (EROs. Se usaron cardiomiocitos aislados de gato. La Ang II, 1 nM, produjo un efecto inotrópico positivo (EIP de 31.8±3.8% que fue cancelado por inhibición de los receptores AT1, de los receptores de ET, del intercambiador Na+/H+ (NHE, del modo inverso del intercambiador Na+/Ca2+ (NCX o por el secuestro de EROs. La Ang II, 100 nM, produjo un EIP de 70.5±7.6% que fue cancelado por inhibición de los receptores AT1 y bloqueado en parte por inhibición de los receptores de ET, del NHE, del modo inverso del NCX o por el secuestro de EROs. La Ang II, 1 nM, incrementó el ARNm de la preproET-1 lo cual fue anulado por el bloqueo de los receptores AT1. Los resultados permiten concluir que el EIP de la Ang II es debido a la acción de la ET-1 endógena liberada/formada por la Ang II. La ET-1 produce: estimulación del NHE, activación del modo inverso del NCX y un consecuente EIP. Dentro de esta cascada también participarían los EROs.Many of the effects thought to be due to angiotensin II (Ang II are due to the release/formation of endothelin (ET. We tested whether Ang II elicits its positive inotropic effect (PIE by the action of endogenous ET-1 and the role played by the reactive oxygen species (ROS in this mechanism. Experiments were performed in cat isolated ventricular myocytes in which sarcomere shortening (SS was measured to asses contractility after pharmacological interventions and the effect of Ang II on inotropism were analyzed. Ang II 1 nM increased SS by 31.8±3.8% (p<0.05. This PIE was cancelled by AT1 receptor blockade, by ET-1 receptors blockade, by Na+/H+ exchanger (NHE inhibition, by reverse mode Na+/Ca2

Absorción renal de agua: del nefrón aislado a los canales de agua a Aquaporinas

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Guillermo Whittembury

1997-10-01

Full Text Available Se revisan brevemente: Los sitios de absorción del filtrado glomerular en diversos segmentos del nefrón, los sitios de acción de la hormona antidiurética (HAD, los conceptos que llevan a proponer que existen canales de agua en el túbulo renal proximal. llamados ahora Aquaporinas (AQP, algunas características biofísicas (diámetro equivalente y longitud del filtro de selectividad de la AQP-l en el túbulo renal proximal, estructura molecular de las AQP, algunas características de las AQP- 1-5, localización de las AQP 1-4 en el nefrón y algunos ejemplos de "fisiopatología molecular", que muestran que fallas genéticas en etapas de la acción de HAD en los túbulos colectores de los nefrones permiten describir varias formas de "diabetes insípida" renal. Hay métodos para dosar AQP-2 en orina. Esto simplificará el diagnóstico diferencial entre diversas formas de diabetes insípida. Estos hallazgos han sido posibles por los avances recientes en la biología molecular de las AQP. Sólo se citan algunas referencias críticas. (Rev Med Hered 1997; 8: 158-161

Immunohistochemical studies on the effect of Aloe vera on the ...

African Journals Online (AJOL)

Address for Correspondence. Immunohistochemical studies on the effect of Aloe vera on the pancreatic β-cells in neonatal streptozotocin-induced type-II diabetic rats. Sema Bolkent 1, Nuriye Akev 2*, Ayşe Can 2, Sehnaz Bolkent 3, Refiye Yanardag 4 &. Alper Okyar 5. 1. Department of Medical Biology, Cerrahpasa Faculty ...

Aloe; Beyond use as cosmetics | Pili | Dar Es Salaam Medical ...

African Journals Online (AJOL)

Dar Es Salaam Medical Students' Journal. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 15, No 1 (2008) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. Aloe; Beyond use as cosmetics. K Pili. Abstract.

Effect of Gelatin-Based Edible Coatings Incorporated with Aloe vera and Black and Green Tea Extracts on the Shelf Life of Fresh-Cut Oranges

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Mohsen Radi

2017-01-01

Full Text Available The aim of this study was to evaluate the effect of gelatin coating incorporated with Aloe vera gel (50,100% and green and black tea extracts (5,10% on physicochemical, microbial, and sensorial properties of fresh-cut oranges at 4°C for 17 days. Significant differences in terms of quality parameters were observed between the control and coated fresh-cut oranges. The highest variation of quality parameters was observed in control, while the least variations were observed in coated slices with 100% Aloe vera and 10% green tea extract. The weight loss was increased with time, but the coating treatment especially with 100% Aloe vera had significant effect on the prevention of weight loss. Also, Aloe vera coated samples obtained the highest score in sensory evaluation. Coating with gelatin incorporated with Aloe vera and green tea extracts successfully retarded the microbial growth and therefore extended the shelf life of fresh-cut oranges during cold storage.

Bacillus sp. G3 un microorganismo promisorio en la biorremediación de aguas industriales contaminadas con cromo hexavalente

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Alexander Mora Collazos

2016-01-01

Full Text Available Introducción: El presente trabajo tuvo como objetivo identificar y caracterizar microorganismos provenientes de un agua residual industrial potencialmente útiles en biorremediación de aguas contaminadas con cromo hexavalente. Metodología: Se realizó el aislamiento de tres microorganismos partiendo de un agua residual industrial proveniente de una empresa de galvanoplastia. Se evaluó el potencial de los microorganismos aislados en su capacidad para reducir cromo hexavalente; escogiéndose al aislado bacteriano G3 por mostrase potencialmente útiles en biorremediación de cromo hexavalente. Se realizó la caracterización morfológica, bioquímica e inferencias filogenéticas basadas en el gen del ARNr 16S del aislado seleccionado; se evaluó el potencial de reducción de cromo hexavalente asociado a la actividad microbiana y la actividad enzimática utilizando un extracto libre de células; de forma adicional, se realizaron bioensayos de reducción de cromo evaluando la acción conjunta de los 3 microorganismos. Resultados: Los datos moleculares y pruebas bioquímicas sugieren que el asilado bacteriano G3 pertenece al grupo de Bacillus cereus sensu lato. Los bioensayos de reducción de cromo hexavalente (10 mg/L realizados con Bacillus sp. G3 mostraron una reducción química del 100% del contaminante al cabo de diez horas en medio LB y de 34 horas en agua residual industrial. El extracto libre de células obtenido a partir del aislado bacteriano G3 mostró actividad de cromato reductasa al reducir el cromo hexavalente a su forma trivalente, mostrando valores óptimos de actividad relativa a 28ºC y 6.5 de pH. La acción conjunta de los microorganismos sobre la reducción de cromo se mostró cinco veces menos eficiente que los ensayos realizados solo con la cepa G3. Discusión o conclusión: Los resultados sugieren, que el aislado bacteriano G3 se muestra como un microorganismo con buen potencial para utilizarse en procesos de biorremediaci

Formación de un panel de cata especializado en carne de vacuno : selección de catadores y entrenamiento del grupo

OpenAIRE

Oliván, M.C. (María); Guerrero, Luis

2012-01-01

Este trabajo trata del seguimiento para la formación de un panel de análisis sensorial aplicado a la carne de vacuno. Se basa en un conjunto de técnicas que permiten valorar las propiedades de los alimentos, es decir, los atributos de alos alimentos que se pueden detectar por los sentidos.

Recaudación de rentas, control contable y ejecución del gasto. Las reformas combinadas de la renta del tabaco y la Tesorería General en el Siglo XVIII español

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Sergio Solbes Ferri

2016-12-01

trabajados por la historiografía reciente relativa al siglo XVIII español. La primera se constituye como ejemplo paradigmático para el análisis de la recaudación de rentas reales; la segunda resulta fundamental para explicar el modo de ejecución del gasto conjunto de la monarquía hispánica. Sin embargo, ambos espacios han quedado historiográficamente aislados en una dualidad referida a ingresos y gastos, sin conexión alguna entre ellos. El presente artículo pretende superar dicha separación para analizar cómo el producto de las rentas reales acaba siendo gestionado por la Tesorería General bajo un solo cargo y una sola data. Se estudia la ordenación administrativa y contable de ambos organismos, así como el procedimiento por el que los caudales del estanco general quedan a disposición de la Tesorería General.

The Effects of Aloe vera Cream on the Expression of CD4+ and CD8+ Lymphocytes in Skin Wound Healing.

Science.gov (United States)

Prakoso, Yos Adi; Kurniasih

2018-01-01

The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150-200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy. Skin specimens were prepared for histopathological study using H&E stain and IHC stain against CD4 + and CD8 + lymphocytes. All the data were analyzed using SPSS16. The result showed that topical application of 1% and 2% Aloe vera cream significantly reduced the percentage of the wound, leucocytes infiltration, angiogenesis, and expression of CD8 + lymphocytes and increased the epidermal thickness and the expression of CD4 + lymphocytes ( p ≤ 0,05). There was no significant difference in the number of fibroblasts in all groups. Topical application of 1% and 2% Aloe vera cream has wound healing potential via their ability to increase the ratio of CD4 + /CD8 + lymphocytes in the wound area.

Quantitative Analysis of Aloins and Aloin-Emodin in Aloe Vera Raw Materials and Finished Products Using High-Performance Liquid Chromatography: Single-Laboratory Validation, First Action 2016.09.

Science.gov (United States)

Kline, David; Ritruthai, Vicha; Babajanian, Silva; Gao, Quanyin; Ingle, Prashant; Chang, Peter; Swanson, Gary

2017-05-01

A single-laboratory validation study is described for a method of quantitative analysis of aloins (aloins A and B) and aloe-emodin in aloe vera raw materials and finished products. This method used HPLC coupled with UV detection at 380 nm for the aloins and 430 nm for aloe-emodin. The advantage of this test method is that the target analytes are concentrated from the sample matrix (either liquid or solid form) using stepwise liquid-liquid extraction (water-ethyl acetate-methanol), followed by solvent evaporation and reconstitution. This sample preparation process is suitable for different forms of products. The concentrating step for aloins and aloe-emodin has enhanced the method quantitation level to 20 parts per billion (ppb). Reversed-phase chromatography using a 250 × 4.6 mm column under gradient elution conditions was used. Mobile phase A is 0.1% acetic acid in water and mobile phase B is 0.1% acetic acid in acetonitrile. The HPLC run starts with a 20% mobile phase B that reaches 35% at 13 min. From 13 to 30 min, mobile phase B is increased from 35 to 100%. From 30 to 40 min, mobile phase B is changed from 100% back to the initial condition of 20% for re-equilibration. The flow rate is 1 mL/min, with a 100 μL injection volume. Baseline separation (Rs > 2.0) for aloins A and B and aloe-emodin was observed under this chromatographic condition. This test method was validated with raw materials of aloe vera 5× (liquid) and aloe vera 200× (powder) and finished products of aloe concentrate (liquid) and aloe (powder). The linearity of the method was studied from 10 to 500 ppb for aloins A and B and aloe-emodin, with correlation coefficients of 0.999964, 0.999957, and 0.999980, respectively. The test method was proven to be specific, precise, accurate, rugged, and suitable for the intended quantitative analysis of aloins and aloe-emodin in raw materials and finished products. The S/N for aloins A and B and aloe-emodin at 10 ppb level were 12, 10, and 8

Survey of chemical manure on morphological traits in Iranian Aloe ...

African Journals Online (AJOL)

This experiment was conducted at the Institute of National Genetic Engineering and Biotechnology in Iran to evaluate the effect of different amount of fertilizers on the leaf and plant characteristics, as well as the yield characteristics of Aloe vera. There were 6 different treatments viz., T1 = 100% soil (control), T2 = 100 PK ...

Antimalarial Anthrone and Chromone from the Leaf Latex of Aloe ...

African Journals Online (AJOL)

In Ethiopian traditional medicine, the leaf latex of Aloe debranan Chrstian is used for the treatment of several diseases including malaria. In an ongoing search for effective, safe and cheap antimalarial agents from plants, the leaf latex of A. debrana was tested for its in vivo antimalarial activity, in a 4-day suppressive assay ...

Botanical aspects of aloes of north East Africa | Demissew | Bulletin ...

African Journals Online (AJOL)

Bulletin of the Chemical Society of Ethiopia. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 10, No 1 (1996) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. Botanical aspects of aloes of north East Africa.

A randomized double-blind, placebo-controlled efficacy and safety study of ALO-02 (extended-release oxycodone surrounding sequestered naltrexone) for moderate-to-severe chronic low back pain treatment.

Science.gov (United States)

Rauck, Richard L; Hale, Martin E; Bass, Almasa; Bramson, Candace; Pixton, Glenn; Wilson, Jacquelyn G; Setnik, Beatrice; Meisner, Paul; Sommerville, Kenneth W; Malhotra, Bimal K; Wolfram, Gernot

2015-09-01

The objective of this multicenter, double-blind, placebo-controlled, randomized withdrawal study was to evaluate the efficacy and safety of ALO-02, an abuse-deterrent formulation containing pellets of extended-release oxycodone hydrochloride (HCl) surrounding sequestered naltrexone HCl, compared with placebo in the treatment of moderate-to-severe chronic low back pain. An open-label titration period in which all patients received ALO-02 was followed by a double-blind treatment period where patients meeting treatment response criteria were randomized to either a fixed dose of ALO-02 or placebo. Daily average low back pain was assessed using an 11-point numeric rating scale (NRS)-Pain. Of the 663 patients screened, 410 received ALO-02 during the open-label conversion and titration period and 281 patients were randomized to the double-blind treatment period (n = 134, placebo; n = 147, ALO-02). Change in the mean NRS-Pain score from randomization baseline to the final 2 weeks of the treatment period was significantly different favoring ALO-02 compared with placebo (P = 0.0114). Forty-four percent of patients treated with placebo and 57.5% of patients treated with ALO-02 reported ≥30% improvement in weekly average NRS-Pain scores from screening to the final 2 weeks of the treatment period (P = 0.0248). In the double-blind treatment period, 56.8% of patients in the ALO-02 group and 56.0% of patients in the placebo group experienced a treatment-emergent adverse event (TEAE). The most common treatment-related TEAEs for ALO-02 during the treatment period were nausea, vomiting, and constipation, consistent with opioid therapy. ALO-02 has been demonstrated to provide significant reduction of pain in patients with chronic low back pain and has a safety profile similar to other opioids.

Preparation and characterization of chitosan/Aloe Vera composite nanofibers generated by electrostatic spinning

Energy Technology Data Exchange (ETDEWEB)

Ibrahim, Illani; Sekak, Khairunnadim Ahmad; Hasbullah, Norazurean [Center of Physics and Material Studies, Faculty of Applied Sciences, Universiti Teknologi Mara (UiTM) 40450 Shah Alam, Selangor (Malaysia)

2015-08-28

Researches on the fabrication of nanostructured based membrane have attracted great attention amongst scientists due to their wide potential applications on medical application. In this work, Chitosan and Aloe Vera sol-gel solution were electrospun using 20 kV DC supply at room temperature. Morphological structure and functional group of nanofibers were characterized using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FT-IR) respectively. The optimum parameter obtained at 90% concentration of acetic acid, 0.3 ml/h of solution flow rate and 10 cm distance of nozzle to collector. The fiber diameters were analyzed using the ImageJ software. Average diameters of the Chitosan/Aloe Vera composite nanofibers is 183nm in ranges of 140–260nm.

Preparation and characterization of chitosan/Aloe Vera composite nanofibers generated by electrostatic spinning

International Nuclear Information System (INIS)

Ibrahim, Illani; Sekak, Khairunnadim Ahmad; Hasbullah, Norazurean

2015-01-01

Researches on the fabrication of nanostructured based membrane have attracted great attention amongst scientists due to their wide potential applications on medical application. In this work, Chitosan and Aloe Vera sol-gel solution were electrospun using 20 kV DC supply at room temperature. Morphological structure and functional group of nanofibers were characterized using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FT-IR) respectively. The optimum parameter obtained at 90% concentration of acetic acid, 0.3 ml/h of solution flow rate and 10 cm distance of nozzle to collector. The fiber diameters were analyzed using the ImageJ software. Average diameters of the Chitosan/Aloe Vera composite nanofibers is 183nm in ranges of 140–260nm

Preparation and characterization of chitosan/Aloe Vera composite nanofibers generated by electrostatic spinning

Science.gov (United States)

Ibrahim, Illani; Sekak, Khairunnadim Ahmad; Hasbullah, Norazurean

2015-08-01

Researches on the fabrication of nanostructured based membrane have attracted great attention amongst scientists due to their wide potential applications on medical application. In this work, Chitosan and Aloe Vera sol-gel solution were electrospun using 20 kV DC supply at room temperature. Morphological structure and functional group of nanofibers were characterized using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FT-IR) respectively. The optimum parameter obtained at 90% concentration of acetic acid, 0.3 ml/h of solution flow rate and 10 cm distance of nozzle to collector. The fiber diameters were analyzed using the ImageJ software. Average diameters of the Chitosan/Aloe Vera composite nanofibers is 183nm in ranges of 140-260nm.

Acción de la miotoxina del veneno de Bothrops brazili Hoge, 1953 (Ophidia: Viperidae

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Carmen Pantigoso

2013-06-01

Full Text Available Se ha estudiado el modo de acción de la miotoxina aislada del veneno de la serpiente Bothrops brazili. La inoculación de la miotoxina en el músculo gastrocnemius de ratones albinos produce durante la primera hora de acción la liberación de creatina kinasa y lactato deshidrogenasa, mientras que por PAGE-SDS, se revela que la incubación de la miotoxina con músculo gastrocnemius aislado, produce además la liberación de otras proteínas musculares. Asimismo, la miotoxina produce hipercontracción, lesiones delta e incrementa los niveles de calcio intramuscular, tanto in vivo como in vitro, lo cual no depende del ingreso de calcio extracelular vía receptores de dihidropiridina. Este incremento de calcio explicaría la hipercontracción observada y podría generar la activación de proteasas y lipasas endógenas dependientes de calcio, que conducirían a la necrosis muscular.

Responses of broilers to Aloe vera bioactives as feed additive: The effect of different forms and levels of bioactives on performances of broilers

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A.P Sinurat

2002-06-01

Full Text Available Feed additives are commonly used in poultry feed as growth promotors or to improve feed efficiency. Previous results showed that Aloe vera bioactives could improve feed efficiency in broilers. Therefore, a further study was designed in order to obtain optimum doses and application methods of bioactives for broiler chickens. Aloe vera was prepared in different forms (fresh gel, dry gel, fresh whole leaf or dry whole leaf. The aloe was supplemented into the feed with concentrations of 0.25; 0.5 and 1 g/kg (equal to dry gel. Standard diets with or without antibiotics were also included as control. The diets were fed to broilers from day old to 5 weeks and the performances were observed. Results showed that the aloe-bioactives did not significantly (P>0.05 affect final body weight of broilers as compared with the control. Supplementation of 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel significantly improved feed convertion by 4.7; 4.8 and 8.2%, respectively as compared with the control. This improvement was a result of reduction in feed intake or dry matter intake without reducing the weight gain. However, supplementation of whole aloe leafs could not improve feed convertion in boilers. It is concluded that the bioactives of Aloe vera could be used as feed supplement to improve feed efficiency in broilers with no deleterious effect on weight gain, carcass yield, abdominal fat levels and internal organs. The effective concentrations of aloe gell as a feed supplement based on dry matter convertion were from 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel.

Perfil fenotípico e genotípico de Staphylococcus aureus isolados de estudantes de enfermagem, 2008 Perfil fenotípico y genotípico del Staphylococcus aureus aislados de estudiantes de enfermería, 2008 Phenotypic and genotypic profile of Staphylococcus aureus isolated in nursing students, 2008

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Suelen Teixeira Faria

2011-01-01

Full Text Available OBJETIVO: Verificar a prevalência de carreamento nasal, perfil fenotípico e genotípico de S. aureus isolados de estudantes de enfermagem. MÉTODOS: Estudo transversal, com população composta por 101 alunos, cursando as três primeiras séries do curso de graduação em Enfermagem no ano de 2008. S. aureus foi isolado de material biológico obtido dos vestíbulos nasais através de swab. A susceptibilidade à oxacilina e vancomicina foi determinada pelo teste de concentração inibitória mínima. A presença do gene MecA foi determinada pelo teste de reação em cadeia da polimerase. RESULTADOS: Verificou-se 90,1% de positividade para S. aureus. A frequência de resistência à oxacilina foi de 9,8% e todas as amostras foram sensíveis à vancomicina. A oito amostras resistentes À oxacilina apresentaram o gene MecA. CONCLUSÃO: A prevalência foi elevada. A resistência à oxacilna foi expressiva e todas as amostras foram sensíveis à vancomicina. As amostras resistentes à oxacilina carreavam o gene MecA.OBJETIVO: Verificar la prevalencia de transporte nasal, perfil fenotípico y genotípico de S. aureus aislados de estudiantes de enfermería. MÉTODOS: Estudio transversal, con población compuesta por 101 alumnos, cursando las tres primeras series del Pregrado en Enfermería en el año 2008. El S. aureus fue aislado del material biológico obtenido de los vestíbulos nasales a través de swab. La susceptibilidad a la oxacilina y vancomicina fue determinada por el test de concentración inhibitoria mínima. La presencia del gen MecA fue determinada por el test de reacción en cadena de la polimerasa. RESULTADOS: Se verificó el 90,1% de positividad para el S. aureus. La frecuencia de resistencia a la oxacilina fue de 9,8% y todas las muestras fueron sensibles a la vancomicina. Las ocho muestras resistentes a la oxacilina presentaron el gen MecA. CONCLUSION: La prevalencia fue elevada. La resistencia a la oxacilina fue expresiva y todas

Phase III double-blind evaluation of an aloe vera gel as a prophylactic agent for radiation-induced skin toxicity

International Nuclear Information System (INIS)

Williams, Maureen S.; Burk, Mary; Loprinzi, Charles L.; Hill, Mary; Schomberg, Paula J.; Nearhood, Kim; O'Fallon, Judith R.; Laurie, John A.; Shanahan, Thomas G.; Moore, Randy L.; Urias, Rodolfo E.; Kuske, Robert R.; Engel, Roland E.; Eggleston, William D.

1996-01-01

Purpose: Considerable pilot data and clinical experience suggested that an aloe vera gel might help to prevent radiation therapy-induced dermatitis. Methods and Materials: Two Phase III randomized trials were conducted. The first one was double blinded, utilized a placebo gel, and involved 194 women receiving breast or chest wall irradiation. The second trial randomized 108 such patients to aloe vera gel vs. no treatment. Skin dermatitis was scored weekly during both trials both by patients and by health care providers. Results: Skin dermatitis scores were virtually identical on both treatment arms during both of the trials. The only toxicity from the gel was rare contact dermatitis. Conclusions: This dose and schedule of an aloe vera gel does not protect against radiation therapy-induced dermatitis

Clonaje y caracterización molecular in silico de un transcrito de fosfolipasa A2 aislado del veneno de la serpiente peruana Lachesis muta Molecular cloning and characterization in silico of phospholipase A2 transcripto isolated from Lachesis muta peruvian snake venom

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Karim L. Jimenez

2010-12-01

Full Text Available Objetivo. Aislar y caracterizar in silico un transcrito del gen de fosfolipasa A2 (PLA2 aislado del veneno de Lachesis muta de la Amazonía peruana. Materiales y métodos. Se amplificó el transcrito del gen sPLA2 mediante la técnica de RT-PCR a partir de RNA total utilizando cebadores específicos, el producto de DNA amplificado se insertó en el vector pGEM para su posterior secuenciación. Mediante análisis bioinformático de la secuencia nucleotídica se determinó un marco de lectura abierta de 414 nucleótidos que codifica 138 aminoácidos, incluyendo16 aminoácidos del péptido señal, el peso molecular y el pI fueron de 13 976 kDa y 5,66 respectivamente. Resultados. La secuencia aminoacídica denominada Lm-PLA2- Perú, contiene Asp49, así como Tyr-28, Gly-30, Gly-32, His-48, Tyr52, Asp99 importantes para la actividad enzimática. La comparación de Lm-PLA2-Perú con las secuencias aminoacídicas de los bancos de datos mostró 93% de similitud con las sPLA2 de Lachesis stenophrys y más del 80% con otras sPLA2 de venenos de la familia Viperidae. El análisis filogenético de la secuencia nucleotídica del transcrito del gen sPLA2 indica que Lm-PLA2-Perú se agrupa con otras sPLA2 [Asp49] ácidas previamente aisladas del veneno de Bothriechis schlegelii con un 89% de identidad. El modelaje tridimensional de Lm-PLA2-Perú, presenta una estructura característica de sPLA2 del Grupo II formada por tres hélices-α, una lámina-β, una hélice corta y un lazo de unión con calcio. Conclusión. La secuencia nucleotídica corresponde al primer transcripto del gen de PLA2 clonado a partir del veneno de la serpiente Lachesis muta, que habita en la selva del Perú.Objective. Isolate and characterize in silico gene phospholipase A2 (PLA2 isolated from Lachesis muta venom of the Peruvian Amazon. Material and methods. Technique RT-PCR from total RNA was using specific primers, the amplified DNA product was inserted into the pGEM vector for

Distribution, diversity and conservation of the genus Aloe in Kenya ...

African Journals Online (AJOL)

The diversity patterns indicate a high concentration of taxa in three areas that are identified as Aloe hot spots and thus of high priority for conservation of the genus: The Kulal–Nyiro–Ndotos–Marsabit area in the north, the Taita-Shimba Hills zone to the southeast and the Naivasha–Baringo area in the Rift Valley. Most of the ...

Preliminary Antiplaque Efficacy of Aloe Vera Mouthwash on 4 Day ...

African Journals Online (AJOL)

Background: Due to increasing resistance to antibiotics and rising incidence of oral diseases, there is a need for alternative treatment modalities to combat oral diseases. The aim of the present study was to access the effect of Aloe vera mouthwash on the dental plaque in the experimental period of 4 days and to compare it ...

Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par. Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L.

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Lozada Betty Stefany

2012-11-01

Full Text Available

Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs (AE1, AE2, AE3, AE4 y AE5 extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5 fueron caracterizados por su morfología, germinación y crecimiento en medios de cultivo. La actividad antifúngica de diferentes concentraciones de los AEs fue evaluada contra el aisla- do M2 y la cepa de M. roreri (ATCC 64239, para determinar su efecto sobre la germinación y la inhibi- ción del crecimiento micelial. Los AEs estudiados inhibieron 100% de la germinación y del crecimiento micelial cuando fueron utilizados en concentraciones de 800 - 1000 µg/ml. Concentraciones de 200 µg/ml también mostraron efecto sobre los aislamientos fúngicos, siendo los AEs obtenidos de L. ori- ganoides (AE2 y AE3 los más activos. Estos estaban compuestos principalmente por timol, p-cimeno,

y-terpineno, acetato de timilo, carvacrol, 1-mirceno, trans-1ccariofileno. Diferencias significativas (P <

EVALUACIÓN DE UN RECUBRIMIENTO FORMULADO CON BASE EN PINTURA INDUSTRIAL Y EXTRACTO DE Aloe saponaria EXPUESTO A LA ATMÓSFERA MARINA I EVALUATION OF A COATING MADE FROM INDUSTRIAL PAINT BASE AND EXTRACT OF Aloe saponaria EXPOSED TO MARINE ATMOSPHERE

OpenAIRE

Asyuri del Valle Roquez Ramos; Solange Ysbeth Paredes Dugarte

2018-01-01

A base of industrial paint mixed with extract of plants of the genus Aloe was evaluated as a possible corrosion inhibiting coating. A phosphate buffered saline solution (BFS) was used to prepare the aqueous extract of Aloe saponaria and mixtures were prepared based industrial paint and the aqueous extract at different ratios (80:20, 70:30, 60:40, 50:50). Plates of AA3003 aluminum alloy were coated and exposed to the marine atmosphere using the Sucre Atmospher...

Judíos, indios y el mito del crimen ritual. El caso de Chamula, Chiapas, 1868

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Misgav Har Peled

2015-01-01

Full Text Available Entre 1867 y 1869 se vivió en Los Altos de Chiapas la rebelión de los indios chamulas contra los ladinos de San Cristóbal de Las Casas conocida como Guerra de Castas. Según la historia oficial, los indígenas crucificaron el Viernes Santo de 1868 a un niño de dicha comunidad tsotsil. Este hecho puede compararse con la “leyenda de sangre” imputada a los judíos, y su transferencia a los indios suscita preguntarse si se trata de un suceso aislado, o si responde a una lógica que vincula al indio salvaje con el judío como el asesino del Hijo de Dios.

Effectiveness of Aloe vera leaf extract against low level exposure to gamma radiation induced injury in intestinal mucosa of Swiss mice

International Nuclear Information System (INIS)

Gehlot, Prashasnika; Saini, M.R.

2004-01-01

Full Text: Human beings can not deny the presence of all sorts of incoming radiations, which are detrimental to life. The small intestine represents one of the major dose limiting normal tissues in radiotherapy because of its high radio sensitivity. The purpose of this study was to determine the effects of Aloe vera, a potential radioprotector. Radioprotective efficacy of aloe vera leaf extract in intestinal mucosa in mice (1 g/kg body weight/day) was studied from 6h to day 20 after gamma irradiation (0.5 Gy(. Villus height, goblet cells/villus section, total cells are good parameters for the assessment of radiation damage. The mice selected from inbreed colony were divided into two groups. The first group was given Aloe vera extract orally for 15 consecutive days and served as experimental group. On 15th day, after 30 min of above treatment animals of both the groups were exposed to 0.5 Gy gamma irradiation and autopsied on 6, 12, 24 h and 5, 10, 20 days. Aloe vera pretreatment resulted in a significant increase (p<0.001) in villus height, total cells whereas globlet cells showed a significant decrease (p<0.001) from respective irradiated controls at each autospy day. The results suggest that Aloe vera pretreatment provides protection against radiation-induced alterations in intestinal mucosa of Swiss mice

In vitro activity of Allium sativum and Aloe vera extract against ...

African Journals Online (AJOL)

The rapid increase of antibiotic resistance needs to be taken as a threat to both animals and human being. In this study the arbitrary concentration of 25%, 50% and 75% of individual and combined Allium sativum and Aloe vera were tested against Salmonella Gallinarum. The antimicrobials were extracted using aqueous ...

The assessment of crystals derived from Aloe spp. for potential use ...

African Journals Online (AJOL)

Dagginess predisposes sheep to breech strike and can be controlled with management practices (e.g. mulesing, crutching) or by treating the animal with an anthelmintic. The effect of regular treatment with crystals derived from Aloe spp as a natural anthelmintic was assessed in yearling Merino progeny born in 2004 (Trial ...

Role of Natural Immunomodulator (Aloe Vera in Cellular and Humoral Immune Responses

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Ening Wiedosari

2007-12-01

Full Text Available Aloe vera belongs to a group of Liliaceae family plant and cultivated worldwide. It possesses acemannan (acetylated mannan, which has a significant pharmacological property. The acemannan has an immunomodulatory activity when administered to animals. The major immunomodulating effect includes the activation of immune effector cells, such as lymphocytes and macrophages, resulting in the production of cytokines, interleukin (IL-1, IL-6, IL-12 and tumor necrosis factor alpha (TNFα. In particular, this extract can modulate the differentiation capacity of CD4+T cells to mature into Th1 subsets and enhance the innate cytokine response. As a consequence, this extract will have a profound effect in controlling disease, caused by intracellular infectious agents (bacteria and viruses. However, further studies are needed to determine the immunomodulating effects of Aloe vera in multi-component extracts equivalent to what are being used commonly in traditional medicine.

Effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing in rats.

Science.gov (United States)

Eamlamnam, Kallaya; Patumraj, Suthiluk; Visedopas, Naruemon; Thong-Ngam, Duangporn

2006-04-07

To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing. Male Spraque-Dawley rats (n=48) were divided into four groups. Group1 served as control group, group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-alpha and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly (P<0.05) increased in the ulcer groups when compared to the control group. The level of TNF-alpha was elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera, leukocyte adherence was reduced in postcapillary venule. The level of IL-10 was elevated, but the level of TNF-alpha had no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-alpha were still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-alpha level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment. Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-alpha level and reduce

Effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing in rats

Institute of Scientific and Technical Information of China (English)

Kallaya Eamlamnam; Suthiluk Patumraj; Naruemon Visedopas; Duangporn Thong-Ngam

2006-01-01

AIM: To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing.METHODS: Male Spraque-Dawley rats (n=48) were divided into four groups. Group1 served as control group,group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-α and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. RESULTS: On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly (P＜ 0.05) increased in the ulcer groups when compared to the control group. The level of TNF-αwas elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera,leukocyte adherence was reduced in postcapillary venule.The level of IL-10 was elevated, but the level of TNF-αhad no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-αwere still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-α level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment.CONCLUSION: Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-α level

Aloe vera Leaf Anti Inflamation’s Activity Speeds Up the Healing Proccess of Oral Mucosa Ulceration

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Agoeng Tjahajani

2013-05-01

Full Text Available Previous work showed that 25% of inner layer Aloe vera leaf extract was effective as anti-inflammatory on the oral mucous of Wistar rats. Objective: The aim of this study was to examine the lowest concentration of whole leaf Aloe vera extract that could accelerate healing process of ulceration on rats. Methods: Sixty inbreeding Spraque Dawley rats was exposed to solution of Hydrogen Peroxide 10% during three days to induced inflammation on the labial mucous inferior of rats. During the next three days, on the same area of control group were topically applied solution of Natrium Chloride 0.9%; while in the treatment group were topically applied 6.25%; 12.5%; 25% of whole leaf of Aloe vera extract for 3x5 minutes with 90 minutes intervals. On the fourth day, five rats in each group were sacrificed; and the same treatment was done on others groups. On the sixth day, five rats of each group were sacrificed; and the same treatment was done on the rest group up to seven days. On the eighth days, the rest groups were sacrificed. Microscopic slides were done. Results: Microscopic slides were analyzed under light microscope and scored. Statistical analysis with Mann-Whitney test showed significant differences between control and treatment group (p<0.05. Conclusion: Application of 6.25% whole leaf Aloe vera extract was the lowest and the most effective concentration in accelerating the healing process of oral mucous ulceration on rats.DOI: 10.14693/jdi.v18i1.56

Características del muestreo microbiológico realizado a aguas residuales en Puerto Padre

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Ana Julia Lozada Pérez

2015-01-01

Full Text Available La vigilancia de aguas residuales, a través del muestreo microbiológico de las mismas, es un importante indicador sanitario, cuando se evalúa la calidad de vida en poblaciones humanas. Se realizó un estudio descriptivo retrospectivo, con el objetivo de caracterizar el muestreo microbiológico en aguas residuales del municipio de Puerto Padre durante el año 2014. Se trabajó con el total de muestras tomadas en aguas residuales, según los puntos establecidos en el municipio, y que fueron analizadas en el laboratorio de microbiología del Centro Municipal de Higiene y Epidemiología. Se analizaron la positividad de la vigilancia microbiológica de aguas residuales según la época del año, el método de recolección de muestra empleado y los tipos de microorganismos patógenos aislados. Del total de 123 muestras tomadas, 29 fueron positivas, para un 23,58 % y los meses de mayor positividad fueron noviembre y diciembre, con 28,57 %. Con el método del hisopo se tomaron 70 muestras y 18 fueron positivas (25,71 %, mientras que por cucharón se tomaron 53 muestras y 11 fueron positivas (20,75 %. El agente de mayor aislamiento en las muestras tomadas fue el Vibrión n 001, con un 47,8 %, seguido la Plesiomona ssp, con 21,7 %

Natural hybrids in the genus of Aloe (Aloeaceae) in East Africa ...

African Journals Online (AJOL)

Natural hybrids can arise in the genus Aloe because their chief pollinators, sunbirds, are not species-specific when feeding on nectar. From literature and the observations of the writer and others, 16 cases of known or suspected natural interspecific hybrids in East Africa are recorded. Journal of East African Natural History ...

Assessment of the safety of aqueous extract of Aloe vera on ...

African Journals Online (AJOL)

Aghomotsegin

2015-07-29

Jul 29, 2015 ... Aloe vera is used both traditionally and packaged commercially in many regions of the world for several medicinal and or cosmetic purposes. It is claimed to have rejuvenating, moisturizing, healing or soothing properties on the skin and gastrointestinal tract. This study focused on assessment of the safety of ...

Assessment of the Safety of Aqueous Extract of Aloe vera on ...

African Journals Online (AJOL)

Aloe vera is used both traditionally and packaged commercially in many regions of the world for several medicinal and or cosmetic purposes. It is claimed to have rejuvenating, moisturizing, healing or soothing properties on the skin and gastrointestinal tract. This study focused on assessment of the safety of A. vera on blood ...

Expression of biologically active human interferon alpha 2 in aloe vera

Science.gov (United States)

We have developed a system for transgenic expression of proteins in Aloe Vera. Using this approach we have generated plants expressing the human gene interferon alpha 2, IFNa2. IFNa2 is a small secreted cytokine that plays a vital role in regulating the body’s immune response to viral infections a...

Potencial biofertilizante de bacterias diazótrofas aisladas de muestras de suelo rizosférico

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Laura Y Moreno

Full Text Available Las bacterias promotoras del crecimiento vegetal son capaces de adaptarse, colonizar y persistir en la rizosfera de la planta, lo cual favorece su crecimiento y desarrollo. En este trabajo se aislaron 32 cepas nativas de bacterias diazótrofas, a partir de muestras de suelo rizosférico de diferentes cultivos, identificadas mediante los medios de cultivo tradicionales y el sistema BBL CRYSTAL. La identificación bioquímica se realizó a través de la reacción en cadena de la polimerasa (PCR, del inglés Polymerase Chain Reaction. Nueve aislados se determinaron como Stenotrophomonas maltophilia y uno como Azotobacter vinelandii, de los cuales tres coincidieron en la identificación bioquímica y la molecular. Se realizó un ensayo en invernadero para evaluar el efecto fertilizante en plantas de maíz, según un diseño experimental de bloques al azar y nueve tratamientos, por triplicado: tratamiento 1: A. vinelandii ATCC 9046; tratamientos del 2 al 7 para los seis aislados; tratamiento 8 para fertilización química; y tratamiento 9 para suelo sin fertilización (testigo. Los aislados M8-10, M10-1 y M11-3, identificados como S. maltophilia por PCR, tuvieron mejores resultados en la emergencia de las plantas, el diámetro del tallo y la longitud de las hojas y los tallos. Finalmente se determinó que M10-1 mostró valores de emergencia y crecimiento de la planta por encima de la media y el límite superior, con respecto a los demás aislados y controles, lo cual lo convierte en un potencial biofertilizante.

Four new species of Aloe (Aloaceae) from Ethiopia, with notes on the ethics of describing new taxa from foreign countries

DEFF Research Database (Denmark)

Demissw, Sebsebe; Friis, Ib; Awas, Tesfaye

2011-01-01

Subsequent to the treatment of the Aloaceae, with 38 species of Aloe, in the Flora of Ethiopia (Sebsebe Demissew & Gilbert 1997), four more species, Aloe bertemariae Sebsebe & Dioli (2000), A. friisii Sebsebe & M. G. Gilbert (2000), A. clarkei L. E. Newton (2002) and A. elkerriana Dioli & T. A. M...

Impedance studies of a green blend polymer electrolyte based on PVA and Aloe-vera

Science.gov (United States)

Selvalakshmi, S.; Mathavan, T.; Vijaya, N.; Selvasekarapandian, Premalatha, M.; Monisha, S.

2016-05-01

The development of polymer electrolyte materials for energy generating and energy storage devices is a challenge today. A new type of blended green electrolyte based on Poly-vinyl alcohol (PVA) and Aloe-vera has been prepared by solution casting technique. The blending of polymers may lead to the increase in stability due to one polymer portraying itself as a mechanical stiffener and the other as a gelled matrix supported by the other. The prepared blend electrolytes were subjected to Ac impedance studies. It has been found out that the polymer film in which 1 gm of PVA was dissolved in 40 ml of Aloe-vera extract exhibits highest conductivity and its value is 3.08 × 10-4 S cm-1.

Polysaccharide isolated from Aloe vera gel suppresses ovalbumin-induced food allergy through inhibition of Th2 immunity in mice.

Science.gov (United States)

Lee, Dajeong; Kim, Hyuk Soon; Shin, Eunju; Do, Seon-Gil; Lee, Chong-Kil; Kim, Young Mi; Lee, Min Bum; Min, Keun Young; Koo, Jimo; Kim, Su Jeong; Nam, Seung Taek; Kim, Hyun Woo; Park, Young Hwan; Choi, Wahn Soo

2018-05-01

An allergic reaction occurs when the immune system overreacts to harmless substance called allergen that gains access to the body. Food allergy is a hypersensitive immune reaction to food proteins and the number of patients with food allergy has recently increased. Aloe Vera is used for wellness and medicinal purposes. In particular, Aloe vera has been reported to enhance immunity. However, the effect of Aloe vera on food allergy is not yet known. In this study, we investigated the effects of processed Aloe vera gel (PAG) containing low molecular weight Aloe polysaccharide (AP) on ovalbumin (OVA)-induced food allergy in mice. Allergic symptoms, rectal temperature, and diarrhea were measured in OVA-induced food allergy mice. Other allergic parameters were also analyzed by RT-PCR, ELISA, flow cytometry, and other biochemical methods. As the results, PAG suppressed the decrease of body temperature, diarrhea, and allergic symptoms in OVA-induced food allergy mice. PAG also reduced serum concentrations of type 2 helper T cell (Th2) cytokines (Interleukin-(IL)-4, IL-5, and IL-13) as well as histamine, mast cell protease-1 (MCP-1), and immunoglobulin (Ig)E. PAG blocked the degranulation of mast cells and infiltration of eosinophils in intestine. Furthermore, PAG suppressed the population of Th2 cells in spleen and mesenteric lymph nodes. PAG also increased the production of IL-10 and population of type 1 regulatory T (Tr1) cells in mice with food allergy. Taken together, our findings suggest that PAG suppressed Th2 immune responses through, at least partially, stimulating the secretion of IL-10 in food allergy mice. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Clinical and radiographic evaluation of zinc oxide with aloe vera as an obturating material in pulpectomy: An in vivo study

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Abhishek Khairwa

2014-01-01

Full Text Available Background: Pulp therapy for pulpally involved primary teeth continues to be a challenge to clinicians. One of the major areas of continued research is in the area of finding obturating materials to suit the specific properties of these teeth. Zinc oxide eugenol is used frequently in pulpectomy for the obturation of the primary teeth. Aims: To evaluate clinically and radiographically a mixture of zinc oxide eugenol and aloe vera as an obturating material. Materials and Methods: A total of 50 children, aged between 4 and 9 years, who were screened for unilateral or bilateral carious deciduous molars were studied. Out of these, 15 children were randomly selected for endodontic treatment. Obturation was done with a mixture of zinc oxide powder and aloe vera gel. Clinical and radiographic evaluation was done after 7 days, 1 month, 3 months, 6 months, and 9 months. The data were statistically analyzed. Results and Conclusion: Endodontic treatment using a mixture of zinc oxide powder and aloe vera gel in primary teeth has shown good clinical and radiographic success. A detailed observational study with longer follow-up will highlight the benefits of aloe vera in primary teeth as an obturating medium.

The Effects of Aloe Vera on TNF-a Levels, the Percentage of Nk Cells and Th 17 Cells in Rat That Received Izoniazid and Rifampycin.

Science.gov (United States)

Mawarti, Herin; Rajin, Mukhamad; Asumta, Zulfikar

2017-10-01

The present study was undertaken to investigate the hepatoprotective effect of Aloe vera against side effect of antituberculosis drug. Twenty-five rats will be divided into five groups, namely the control group (without any treatment), the group of rats treated with anti-tuberculosis drugs, and a group of rats were treated antituberculosis drugs and got Aloe vera extract at a dose of 40; 80; and 120 mg/kg body weight. Antituberculosis drugs are isoniazid and rifampicin a dose of 50 mg/kg body weight. Antituberculosis treated group showed significantly increase levels of TNF-a, the percentage of NK cells and the number of Th17 cells compared with the control group ( p 0.05). Aloe vera at first and the third dose lower the number of NK cells compared to the antituberculosis group, although it has not yet reached a significant difference ( p > 0.05). The first dose of Aloe vera was significantly decreased the percentage of Th17 cells compared to the antituberculosis drug group ( p 0.05). It was concluded that administration of Aloe vera can suppress the production of TNF-a and the percentage of Th17 cells as a result of antituberculosis drug administration. Thus, Aloe vera can be a useful alternative to natural materials in the successful treatment of tuberculosis through the inhibition of side effect.

Efficacy of Aloe Vera Supplementation on Prediabetes and Early Non-Treated Diabetic Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Science.gov (United States)

Zhang, Yiyi; Liu, Wen; Liu, Dan; Zhao, Tieyun; Tian, Haoming

2016-06-23

The aim of this study was to evaluate evidence for the efficacy of aloe vera on managing prediabetes and early non-treated diabetes mellitus. We performed a systematic search of PubMed, Embase, and Cochrane Central Register of Controlled Trials until 28 January 2016. A total of five randomized controlled trials (RCTs) involving 415 participants were included. Compared with the controls, aloe vera supplementation significantly reduced the concentrations of fasting blood glucose (FBG) (p = 0.02; weighed mean difference [WMD]: -30.05 mg/dL; 95% confidence interval [CI]: -54.87 to -5.23 mg/dL), glycosylated hemoglobin A1c (HbA1c) (p Aloe vera was superior to placebo in increasing serum high density lipoprotein-cholesterol (HDL-C) levels (p = 0.04). Only one adverse event was reported. The evidence from RCTs showed that aloe vera might effectively reduce the levels of FBG, HbA1c, triglyceride, TC and LDL-C, and increase the levels of HDL-C on prediabetes and early non-treated diabetic patients. Limited evidence exists about the safety of aloe vera. Given the small number and poor quality of RCTs included in the meta-analysis, these results are inconclusive. A large-scale, well-designed RCT is needed to further address this issue.

Antitumor properties and modulation of antioxidant enzymes' activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction.

Science.gov (United States)

El-Shemy, H A; Aboul-Soud, M A M; Nassr-Allah, A A; Aboul-Enein, K M; Kabash, A; Yagi, A

2010-01-01

The aim of this study was to evaluate the potential anticancer properties and modulatory effect of selected Aloe vera (A. vera) active principles on antioxidant enzyme activities. Thus, three anthraquinones (Namely: aloesin, aloe-emodin and barbaloin) were extracted from A. vera leaves by supercritical fluid extraction and subsequently purified by high performance liquid chromatography. Additionally, the N-terminal octapeptide derived from verectin, a biologically active 14 kDa glycoprotein present in A. vera, was also tested. In vivo, active principles exhibited significant prolongation of the life span of tumor-transplanted animals in the following order: barbaloin> octapeptide> aloesin > aloe-emodin. A. vera active principles exhibited significant inhibition on Ehrlich ascite carcinoma cell (EACC) number, when compared to positive control group, in the following order: barbaloin> aloe-emodin > octapeptide > aloesin. Moreover, in trypan blue cell viability assay, active principles showed a significant concentration-dependent cytotoxicity against acute myeloid leukemia (AML) and acute lymphocytes leukemia (ALL) cancerous cells. Furthermore, in MTT cell viability test, aloe-emodin was found to be active against two human colon cancer cell lines (i.e. DLD-1 and HT2), with IC(50) values of 8.94 and 10.78 microM, respectively. Treatments of human AML leukemic cells with active principles (100 microg ml(-1)) resulted in varying intensities of internucleosomal DNA fragmentation, hallmark of cells undergoing apoptosis, in the following order: aloe-emodin> aloesin> barbaloin> octapeptide. Intererstingly, treatment of EACC tumors with active principles resulted in a significant elevation activity of key antioxidant enzymes (SOD, GST, tGPx, and LDH). Our data suggest that the tested A. vera compounds may exert their chemo-preventive effect through modulating antioxidant and detoxification enzyme activity levels, as they are one of the indicators of tumorigenesis. These

NUEVAS VÍAS DE PERMEABILIDAD Y REGULACIÓN DEL pH INTRACELULAR COMO POSIBLES BLANCOS TERAPÉUTICOS EN Plasmodium falciparum

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MARY LUZ LÓPEZ

2008-05-01

Full Text Available Actualmente, existe una necesidad sentida para el desarrollo de nuevos fármacos antimaláricos o de compuestos conocidos dirigidos contra blancos terapéuticos diferentes a los afectados por los medicamentos usuales. Son diversos los blancos que pueden ser aprovechados en Plasmodium, y la alteración de parámetros fisiológicos como el pH y el transporte de solutos pueden explicar la muerte del parásito cuando se usan compuestos antiplasmodiales, lo que representa una opción para el desarrollo de nuevas alternativas antiparasitarias. El propósito de esta revisión es por tanto, proporcionar una visión general de los efectos causados por esteroides, discutiendo el caso específico de los esteroides antiplasmodiales aislados de Solanum nudum y revisar dos procesos fisiológicos importantes en el parásito como posibles blancos terapéuticos, la modificación de permeabilidad del eritrocito infectado y el mantenimiento del pH intracelular de Plasmodium.

Modulatory influence of Aloe vera against radiation and cadmium induced hepatic lesions in Swiss albino mice

International Nuclear Information System (INIS)

Harsha, Radha; Purohit, R.K.

2012-01-01

The major objectives in radiobiology has been the development of agents that can mitigate the damage produced by ionizing radiation to normal tissues and thus reduces the side effects caused by radiation and improvement of cancer radiotherapy. The various agents have drawn attention of researchers as they provide wider acceptability and least side effects. The current study was aimed to investigate the protective effect of Aloe vera against radiation and cadmium induced changes in the liver of Swiss albino mice. For the study healthy male Swiss albino mice (6 to 8 weeks old) were selected from an inbred colony and kept in polypropylene cages. They were provided with standard mice feed and tap water ad libitum. The animals were exposed to 3.0 and 6.0 Gy of gamma radiation with or without cadmium chloride treatment. The animals of experimental groups were administered Aloe vera juice seven days prior to irradiation or cadmium chloride treatment. The animals of each group were autopsied at each post treatment interval of 1, 2, 4, 7, 14 and 28 days of treatment. The various biochemical parameters estimated were total proteins, glycogen, cholesterol, acid and alkaline phosphatase activities, DNA and RNA. After routine procedure, histopathological changes were also observed. The changes in various biochemical parameters were observed in the form of increase of decrease in values. The histopathological changes observed on day-1 after exposure to 3.0 Gy were distortion of hepatic architecture, intracellular oedema, narrower sinusoids, cytoplasmic degranulation, vacuolation and pycnotic nuclei. The changes were more marked on day-4 and continued up to day-14. But on day-28 the sign of recovery was observed. After exposure to a higher dose (6.0 Gy) similar changes were noticed but they were more pronounced and there was late manifestation of recovery. In the combined treatment of radiation and cadmium chloride synergistic effects were observed. The liver of Aloe vera treated

Preventive action of Aloe vera against radiation and cadmium induced haematological changes in Swiss albino mice

International Nuclear Information System (INIS)

Agarwal, Manisha; Purohit, R.K.; Chakrawarti, Aruna; Bhartiya, K.M.

2012-01-01

Haematopoietic organs are markedly sensitive to ionizing radiation due to its proliferate activity. The changes found in the circulating blood are primarily due to damage in the radio sensitive haematopoeitic organs. A very small dose of radiation to a blood forming organ causes an arrest of haematopoiesis with changes in peripheral blood count. Certain trace elements are essential for normal growth and development of organisms but their concentration beyond threshold may produce damage to blood forming organs and tissues thus affecting the peripheral blood. Aloe vera has been claimed to contain several important therapeutic properties including anti cancer effects. Various studies showed the prevention of radiation induced suppression of immunity by Aloe vera components. Having these unique properties, Aloe vera could be used in clinical field as a protector against radiation and heavy metal toxicity in human beings. For the study, six to eight weeks old Swiss albino mice were procured and kept in polypropylene cages. The animals were fed with standard mice feed and water was provided to them ad libitum. Cadmium chloride was administered orally to the animals in drinking water at the dose rate of 20 ppm. The animals were exposed to sub lethal doses of 2.0 Gy and 4.0 Gy of gamma radiation from cobalt 60 source. The Aloe vera was given seven days prior to irradiation and continued up to last autopsy day in experimental animals. Five animals from each group were autopsied by cervical dislocation at each post treatment interval of 1,2,4,7,14 and 28 days. The differential leucocytes count was estimated by preparing smear of the blood. The value of lymphocyte decreased up to day-14 in non drug treated groups thereafter it increased up to day-28 whereas the count decreased up to day-7 in drug treated groups and showed an increasing trend at day-14 which continues up to day-28. The value of monocyte and granulocyte percentage increased up to day-7 in drug treated groups

Caracterización de actinobacterias raras, degradadoras de lignocelulosa: demostración de actividad lacasa en dos aislados de Tsukamurella sp y Cellulosimicrobium sp

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Enrique Luis Revollo Escudero

2012-07-01

Full Text Available Título en ingles: Characterization of lignocelluloses-degrading rare actinobacteria: Demostration of laccase activity in two isolates of Tsukamurella sp and Cellulosimicrobium sp Resumen: Las características fisicoquímicas de la lignina y su compactación con la celulosa han dificultado la explotación biotecnológica de enormes cantidades de biomasa vegetal. Las lacasas constituyen una subfamilia de oxidasas multicobre que intervienen en la despolimerización de la lignina. Si bien han sido ampliamente caracterizadas en los hongos, los estudios de la diversidad y las funcionalidades de las lacasas en los procariotas se han centrado especialmente en isoformas enzimáticas de Streptomyces sp. En este trabajo se aislaron 20 cepas de actinobacterias del suelo. La actividad lacasa de 17 de ellas fue evidenciada en ensayos cualitativos con guayacol y dos cepas seleccionadas fueron caracterizadas en detalle. Las pruebas morfológicas y el análisis de las secuencias del gen 16S rRNA apuntan a que estos dos aislados pertenecen a los géneros Tsukamurella y Cellulosimicrobium. En cultivo sumergido con agitación, AC01 (Tsukamurella sp. expresó una máxima actividad de oxidación de ABTS (2,2’-azino-bis-(3-etilbenzotiazolin-6-sulfonato de 108 U/L. Por otra parte, AC18 (Cellulosimicrobium sp. que había exhibido una actividad oxidativa de guayacol superior a las 16 cepas restantes y demostró ser resistente a niveles tóxicos de cobre, logró un valor máximo de oxidación del ABTS de 0,56 U/L. Estos resultados sugieren que en el aislado AC18 operaría un fenómeno de especificidad de sustrato o de inductor, regulador de la expresión y de la actividad lacasa cuantificable. La caracterización genómica y funcional de las lacasas de nuevas actinobacterias lignocelulósicas ampliará la gama de centros redox con aplicaciones biotecnológicas específicas, además de facilitar el establecimiento de sus relaciones evolutivas con las eucariotas

Effect of Edible Coatings from Aloe vera gel on Citrus sinensis ...

African Journals Online (AJOL)

Extension of the shelf life of orange fruits continues to be a challenge in Nigeria. The search for safe, healthy and environmental friendly treatments has led to increased interest in research into edible and biodegradable films and coatings. In this work, the use of Aloe vera gel as a coating to extend the shelf-life of orange ...

Estudios sobre la fisostigmina (eserina y productos derivados

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Gonzalo Montes D.

1943-01-01

Full Text Available En los últimos años se han desarrollado trabajos que muestran claramente cómo los efectos del sistema nervioso parasimpátíco se producen mediante la liberación de una substancia química en las terminaciones nerviosas, la cual es directamente responsable de la excitación de las células inervadas. Esta substancia tiene acción idéntica a la de la acetilcolina. Como trabajos básicos en el descubrimiento de este mecanismo deben citarse los de Loewi, quien probó que la estimulación del vago en el corazón aislado de la rana producía acetilcolina, mientras que la estimulación del simpático liberaba una substancia semejante a la adrenalina. Este descubrimiento fué luégo confirmado por otros investigadores en otros órganos aislados y ha servido para sentar la teoría hormonal del control autónomo.

Reduction of Fasting Blood Glucose and Hemoglobin A1c Using Oral Aloe Vera: A Meta-Analysis.

Science.gov (United States)

Dick, William R; Fletcher, Emily A; Shah, Sachin A

2016-06-01

Diabetes mellitus is a global epidemic and one of the leading causes of morbidity and mortality. Additional medications that are novel, affordable, and efficacious are needed to treat this rampant disease. This meta-analysis was performed to ascertain the effectiveness of oral aloe vera consumption on the reduction of fasting blood glucose (FBG) and hemoglobin A1c (HbA1c). PubMed, CINAHL, Natural Medicines Comprehensive Database, and Natural Standard databases were searched. Studies of aloe vera's effect on FBG, HbA1c, homeostasis model assessment-estimated insulin resistance (HOMA-IR), fasting serum insulin, fructosamine, and oral glucose tolerance test (OGTT) in prediabetic and diabetic populations were examined. After data extraction, the parameters of FBG and HbA1c had appropriate data for meta-analyses. Extracted data were verified and then analyzed by StatsDirect Statistical Software. Reductions of FBG and HbA1c were reported as the weighted mean differences from baseline, calculated by a random-effects model with 95% confidence intervals. Subgroup analyses to determine clinical and statistical heterogeneity were also performed. Publication bias was assessed by using the Egger bias statistic. Nine studies were included in the FBG parameter (n = 283); 5 of these studies included HbA1c data (n = 89). Aloe vera decreased FBG by 46.6 mg/dL (p aloe vera for significantly reducing FBG (46.6 mg/dL) and HbA1c (1.05%). Further clinical studies that are more robust and better controlled are warranted to further explore these findings.

A randomized, double‑blind clinical study to assess the antiplaque and antigingivitis efficacy of Aloe vera mouth rinse

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Bathini Chandrahas

2012-01-01

Full Text Available Aims: To evaluate the efficacy of Aloe Vera mouth rinse on experimental plaque accumulation and gingivitis. Materials and Methods: In this randomized, controlled, and double-blind study, a total of 148 systemically healthy subjects were screened in the age group of 18-25 years. Finally, 120 subjects were requested to abstain from oral hygiene (tooth brushing for 14 days and used a specially fabricated plaque guard. Following cessation of tooth brushing in the specified area, the subjects were randomly divided into Group A (test group who received 100% Aloe vera, Group B (negative control group who received placebo (distilled water, and Group C (positive control group who received 0.2% chlorhexidine. The rinse regimen began on the 15 th day and continued for 7 days. Plaque accumulation was assessed by Plaque Index (PI and gingivitis was assessed by Modified Gingival Index (MGI and Bleeding Index (BI at baseline (0, 7 th , 14 th , and 22 nd days. Results: There was statistically significant decrease in PI, MGI, and BI scores after the rinse regimen began in both Group A (test group and Group C (chlorhexidine compared with Group B. Mouth wash containing Aloe vera showed significant reduction of plaque and gingivitis but when compared with chlorhexidine the effect was less significant. Conclusion: Aloe vera mouthwash can be an effective antiplaque agent and with appropriate refinements in taste and shelf life can be an affordable herbal substitute for chlorhexidine.

Landslide precursory deformation interpretation using ALOS-2/PALSAR-2 InSAR image along Min River in Maoxien, Sichuan Province, China

Science.gov (United States)

Sato, H. P.

2017-12-01

Maoxien area in Sichuan Province, China has many landslide. For example, landslide (rock avalanche) occurred on the slope in Xinmocun Village in Maoxeien on 24 June 2017. I produced and interpreetd InSAR image using ALOS/PALSAR data observed on 19 Jul 2007-3 Sep 2007 and on 27 Jan 2011-14 Mar 2011, and ALOS-2/PALSAR-2 data observed on 26 Jul 2015-13 Dec 2015 and on 13 Dec 2015-11 Dec 2016. These images give good coherence and it was easy to identify local landslide surface deformation. As a result, e.g., two slopes were estimated to have local landslide surface deformation; one is at 103.936587 deg E and 32.04462 deg N, another is at 103.674754 deg E and 31.852838 N. However, the slope in Xinmocun Village was not identified as landslide precursory deformation. In the poster I will present more InSAR image observed after 11 Dec 2016 and discuss the possibility of local landslide surface deformaton using InSAR image. ALOS/PALSAR and ALOS-2/PALSAR-2 data were provided by JAXA through Landslide Working Group in JAXA and through Special Research 2015-B-02 of Earthquake Research Institute/Tokyo University. This study was supported by KAKENHI (17H02973).

The Impact of Aloe vera and Calendula on Perineal Healing after Episiotomy in Primiparous Women: A Randomized Clinical Trial

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Farideh Eghdampour

2013-11-01

Full Text Available Introduction: Episiotomy is used for enlarging the perineum. Aloe vera and Calendula have been used for treating different diseases from ancient times, limited researches have been done regarding the healing of these plants. Since the effect of their ointment on episiotomy healing has not been studied, this study is being done for determining the impact of Aloe vera and Calendula on episiotomy healing in primiparous women. Methods: This clinical trial involves 111 qualified primiparous women admitted in Lolagar hospital. They were randomly categorized into three groups of control (n=1 and experimental (n=2 groups. The women in experimental group used Aloe vera and Calendula Ointment every 8 hours and the control group used hospital routine on episiotomy for 5 days. The data were collected by demographic questionnaire and redness, edema, ecchymosis, discharge and approximation scale (REEDA which investigated the episiotomy healing before and five days after intervention in two groups. ANOVA, Tukey test, Kruskal-wallis, Chi-square were used for data analysis. Results: The three groups do not have statistically significant different regarding demographic and other intervening variables. Comparing the mean of REEDA in five days after delivery showed statistically significant difference between control and experimental groups.Conclusion: According to the results, using Aloe vera and Calendula ointment considerably increases the speed of episiotomy wound healing so it can be used for quickening the episiotomy healing.

Microorganismos con capacidad degradativa de aceites lubricantes usados, aislados de estratos superficiales de suelos contaminados y optimización de condiciones de crcimiento. Ayacucho 2009

OpenAIRE

Palomino Felices, Sonia Haydeé

2014-01-01

La biorremediación, consiste principalmente en el uso de los organismos nativos (bacterias, hongos, plantas) para descomponer o degradar sustancias peligrosas convirtiéndolas en sustancias de caracteres menos tóxicos o inocuos para el ambiente y la salud humana. La investigación tuvo los siguientes objetivos: a) utilizar los microorganismos aislados de suelos contaminados con hidrocarburos para degradar "aceite lubricante usado" en suelos de experimentación en condiciones de laboratorio, b) e...

Castor e Pólux, modelos para Niso e Euríalo na Eneida?

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Francisco Edi de Oliveira Sousa

2009-09-01

Full Text Available O episódio de Niso e Euríalo expõe uma peculiar amicitia entre dois guerreiros, cuja morte provoca forte efeito patético. Seu modelo principal seria a Doloneia; entre os secundários, mencionam-se: o Rhesus, atribuído a Eurípides; a embaixada para Aquiles na Ilíada; a viagem de Telêmaco na Odisseia; relatos da guerra contra Aníbal nos Anais, de Ênio; a relação entre Orestes e Pílades na Ifigênia em Táuris, de Eurípides. Tais modelos, todavia, não propiciam um exame satisfatório da afeição e do fim que os une. Assim, este estudo discute um substrato poético capaz de iluminar a fidelidade e o modus mortis de Niso e Euríalo: o par Castor-Pólux.

¿Son los errores congénitos del metabolismo causa prevenible de muerte súbita?

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Roberto Álvarez Fumero

Full Text Available El síndrome de muerte súbita del lactante (SMSL no es un proceso aislado, sino un hecho multifactorial y multicausal, del cual cada día se restan alteraciones potencialmente diagnosticables, para pasar a ser un diagnóstico de exclusión. Algunos errores congénitos del metabolismo (ECM han sido asociados al SMSL y/o a episodios aparentemente letales EAL, aunque la mayoría de ellos suelen tener alguna expresión clínica que los hace potencialmente diagnosticables antes de la muerte, lo que permite ser descartados de entre las causas del SMSL. De ellos, los más estrechamente relacionados con el SMSL son los trastornos de la betaoxidación mitocondrial de ácidos grasos. Solo del 3 al 5 % de los SMSL pueden guardar relación con ECM. El mayor impacto que representa el conocimiento de la asociación de SMSL y estos trastornos, es precisamente la posibilidad de su prevención. El personal médico debe conocer que la más eficiente forma de prevenir el SMSL relacionado con los ECM, es la búsqueda activa de lactantes de riesgo al realizar una exhaustiva anamnesis. Estos casos deben ser objeto de estudios metabólicos especializados, para lo cual es imprescindible conocer los requisitos de obtención, conservación y traslado de muestras al Laboratorio de Referencia.

Cálculo de la resistencia a la penetración en hojas de aloe vera (Barbadenses Miller

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Giovanni Torres Charry

2013-12-01

Full Text Available Para caracterizar la resistencia a la penetración de hojas de aloe vera, variedad Barbadenses Miller, se diseñó una máquina y se establecieron los protocolos de medición para desplazamiento y fuerza necesarios para medir la resistencia a la penetración en tres puntos diferentes de las hojas. Los resultados mostraron que no existe una relación lineal entre el desplazamiento del indentador en la hoja y la fuerza de penetración. Por otro lado, se encontró que no existen diferencias en los valores de la fuerza de penetración al evaluar ésta en distintos puntos para una misma hoja. Se hallaron diferencias en los valores obtenidos entre hojas diferentes, lo que permite suponer que existe influencia de las características dimensionales de las hojas en el valor de la fuerza de penetración. Los valores máximos obtenidos para la fuerza de penetración variaron entre 22 y 27 N, para una velocidad de avance de la herramienta de 2.6 mm/s.

Potential effect of fortified pan bread with Aloe vera juice on alloxan ...

African Journals Online (AJOL)

Background: This study was designed to investigate (1) the potential use of Aloe vera juice (AVJ) for fortification of pan bread with evaluates the sensory characteristics and the nutritive value. (2) The possible beneficial role of 5% and 10% AVJ-fortified bread against diabetic rats. Materials and Methods: Bread fortified with ...

Evaluation of the anti-ulcer and toxicity profile of Aloe buettneri in ...

African Journals Online (AJOL)

The anti-ulcerogenic potential of the leaf methanol extract of Aloe buettneri A. Berger was investigated using three methods of gastric lesion induction in experimental Wistar rats (150-200 g) and mice (20-25 g): 1. HCl/ethanol-induced gastic lesions, 2. Indomethacin-HCl/ethanol-induced gastric lesions, and 3, Pylorus ...

Pemetaan Hutan Mangrove di Estuari Sungai Andai, Manokwari Papua Barat Berdasarkan Metode Density Slicing Berbasis Citra Alos Avnir-2

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Christian S. Imburi

2016-09-01

Full Text Available ABSTRAK Secara umum julat nilai spektral objek diperoleh melalui perpustakaan spektral (spectral library atau melalui pengukuran langsung di lapangan dengan alat ukur spectroradiometer, spectrometer, dan spectrophotometer, atau alat-alat sejenis yang mahal serta jarang, sehingga sulit untuk memperoleh julat nilai spektral objek tertentu. Studi tentang Metode Density Slicing Berbasis Citra ALOS AVNIR-2 Untuk Pemetaan Hutan Mangrove di Estuari Sungai Andai, Manokwari Papua Barat dilakukan dengan tujuan menghasilkan prosedur analisis perolehan julat nilai spektral melalui analis histogram band inframerah dekat ALOS AVNIR-2.  Penelitian ini dilakukan melalui dua tahapan metode analisis, yakni: 1 Analisis utama untuk menghasilkan julat nilai spektral, yakni analisis histogram band inframerah dekat ALOS AVNIR-2, dan 2 Analisis tambahan untuk menjelaskan tingkat akurasi julat nilai spektral melalui: (a survei lapangan yang menghasilkan data untuk proses uji akurasi reklasifikasi metode density slicing, serta (b melakukan uji akurasi terhadap peta zonasi mangrove berbasis density slicing. Analisis tersebut dijabarkan melalui pendekatan penginderaan jauh multitingkat, dimana citra QuickBird digunakan sebagai referensi untuk masking citra ALOS AVNIR-2, dan juga untuk validasi peta hasil metode density slicing. Hasil dari penelitian ini adalah prosedur analisis perolehan julat nilai spektral dan julat nilai spektral zonasi genus mangrove.  Akurasi pemetan hutan mangrove untuk metode density slicingmenghasilkan nilai 40%. Nilai tersebut masih belum memenuhi standar ketelitian yang diharapkan. Namun demikian, penelitian ini secara keseluruhan dapat menghasilkan julat nilai spektral, dan hal ini memberikan implikasi terhadap pengembangan prosedur untuk menghasilkan julat nilai spektral objek.   ABSTRACT Generally the range of spectral value objects can be obtained through spectral library or through direct measurement in the field with measuring instrument

Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats

International Nuclear Information System (INIS)

Inpanya, Paichit; Viyoch, Jarupa; Faikrua, Atchariya; Ounaroon, Anan; Sittichokechaiwut, Anuphan

2012-01-01

Delayed healing remains a major clinical problem and here we have sought to develop an improved dressing film comprising 1.95% w/v fibroin and 0.05% w/v aloe gel extract. The tensile strength of dry film was 21.1 ± 0.5 MPa and broke at 1.1 ± 0.2% elongation; corresponding values for wet film were 18.3 ± 1.3 MPa and 1.9 ± 0.1%. The film maintained its shape upon water immersion and the swelling ratio of the dry film was 0.8 ± 0.1 while the water uptake was 43.7 ± 2.6%. After 28 days of incubation in phosphate buffered saline (1 M, pH 7.4, 37 °C), the weight of film was reduced by 6.7 ± 1.1% and the tensile strength and elongation at breaking point (dry state) were 15.4 ± 0.6 MPa and 1.5 ± 0.2%, respectively. Compared to aloe-free fibroin film (2.0% fibroin extract only), the blended film enhanced the attachment and proliferation of skin fibroblasts. The bFGF immunofluorescence of fibroblasts cultured on the blended film appeared greater than those cultured on tissue culture plate or on aloe-free fibroin film while α-smooth muscle actin was maintained. In streptozotocin-induced diabetic rats, the wounds dressed with the blended film were smaller (p <0.05) by day 7 after wounding, compared to untreated diabetic wounds. Histology of repaired diabetic wounds showed the fibroblast distribution and collagen fiber organization to be similar to wounds in normal rats, and this was matched by enhanced hydroxyproline content. Thus, such accelerated wound healing by the blended fibroin/aloe gel films may find application in treatment of diabetic non-healing skin ulcers. (paper)

Perbedaan Efek Pemberian Topikal Gel Lidah Buaya (Aloe vera L. Dengan Solusio Povidone Iodine Terhadap Penyembuhan Luka Sayat Pada Kulit Mencit (Mus musculus

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Nur Atik

2009-06-01

Full Text Available Normal wound healing is a complex and dynamic process. Wound healing process can accelerate, with chemical treatment or natural. The chemical treatment often used in healing process is povidone iodine. For natural treatment, topical application of Aloe vera gel may accelerate the full-thickness wound healing process. The purpose of this research was to evaluate the differences between topical application of povidone iodine and Aloe vera gel for skin wound healing in mice. This study used 18 mice that were divided into three groups. First group was the wounded (control group, the second group was wounded – Aloe vera group, the third group was wounded - povidone iodine group. Full-thickness skin wound were created on the dorsal area of mice in each group. The control group were not given anything, while the second group were given Aloe vera gel twice a day, and the third group were given povidone iodine solution twice a day. At the fifth day, all mice were sacrificed for histologic evaluation and VEGF A expression. Data was obtained by microscopic observation of the wounded skin, based on quantitative parameter: epithelial thickness, total fibroblast, total blood vessels, and VEGF A expression. Then the data was statistically analyzed by using independent samples T test, ANOVA, and Chi square. The result demonstrated that the sum of epithelial thickness, fibroblast, blood vessels, and VEGF A expression in the Aloe vera group is higher than in povidone iodine group. Statistic evaluation showed that there were significant differences between the two groups (p < 0.05, with 95% confidence interval. Based on this result, it can be concluded that the topical administration of Aloe vera gel twice a day is better than povidone iodine with parameter epithelial thickness, total fibroblass, total blood vessels and VEGF A expression.

An evaluation of the impact of aloe vera and licorice extracts on the course of experimental pigeon paramyxovirus type 1 infection in pigeons.

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Dziewulska, D; Stenzel, T; Smialek, M; Tykalowski, B; Koncicki, A

2018-02-01

The progressive decrease in the efficiency of synthetic drugs has prompted research into phytogenic feed additives with potentially immunomodulatory and anti-infective properties. Complex diseases with a mixed etiology, including viral, pose a growing problem in domestic pigeons. The aim of this study was to determine the effectiveness of various doses of aloe vera and licorice extracts on the course of experimental PPMV-1 infection in pigeons. The experiment was performed on pigeons divided into 5 groups, including one control group and 4 experimental groups, which were orally administered aloe vera or licorice extracts at 300 or 500 mg/kg BW for 7 d after experimental inoculation with PPMV-1. On d 4, 7, and 14 after inoculation, cloacal swabs and samples of organs were collected from 4 birds in each group. The samples were analyzed to determine the copy number of PPMV-1 RNA by TaqMan qPCR. The results indicate that licorice and aloe vera extracts inhibited PPMV-1 replication by decreasing viral RNA copy numbers in the examined organs. The most inhibitory effect was observed in pigeons receiving aloe vera extract at 300 mg/kg BW, for which PPMV-1 RNA copy numbers were approximately 7-fold lower (brain), 9-fold lower (kidneys), and 14-fold lower (liver) than in the control group. The results of this study point to the potentially antiviral effects of aloe vera and licorice extracts in pigeons infected with PPMV-1. To the best of our knowledge, this is the first study to investigate the antiviral properties of aloe vera and licorice extracts in domestic pigeons. © The Author 2017. Published by Oxford University Press on behalf of Poultry Science Association.

Physicochemical characterization of microwave assisted synthesis of silver nanoparticles using Aloe Vera (Aloe barbadensis)

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Kuponiyi, Abiola John

Biosynthesis of silver nanoparticles (AgNP) using different biological extracts is gaining recognition for its numerous applications in different disciplines. Although different approaches (physical and chemical) have been used for the synthesis of AgNP, the green chemistry method is most preferable because of its high efficacy, cost effectiveness, and environmental benignity. Aloe Vera (AV) contains chemical compounds (anthraquinones) that are known to possess antibacterial, antivirus and anticancer properties and the extract is a good chemical reduction agent for AgNP. Hence, it was hypothesized that a microwave assisted synthesis will produce highly concentrated, homogeneous, stable and biologically active AgNP. Thus, the main objective of the study was to evaluate the effect of microwave assisted synthesis of AgNP, the effect of pulse laser treatment on size reduction of a microwave synthesized AgNP, and the physicochemical characterization of AgNP synthesized with Aloe Vera water and ethanol extract. The experiment was conducted in two phases. Phase 1 was first conducted to optimize the experimental variables, thus establishing the optimum variables to apply in the second phase. The experiment in Phase 1 was conducted using three-factor factorial experimental design comprised of the following factors: 1) Extraction Solvent, 2) Heating Methods, 3) pH; and their corresponding levels were water and ethanol, conventional and microwave, pH (7, 8, 10 and 12), respectively. All synthesis was conducted at constant temperature of 80°C. Phase II experimental treatments were Laser ablation (0, 5, and 10 min) and Storage time (Week 1, 2 & 3). The Phase I of the results showed that increased AgNP concentrations were significantly (p 0.05) impact the particle size distribution. Hence, the Zeta potential of the particles has values typically ranging between +100 mV to -100 mV, hence indicative of colloidal stability matrix. Furthermore, the Polydispersity indexes of Week 1

An investigation of age and yield of fresh fruit bunches of oil palm based on ALOS PALSAR 2

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Darmawan, S.; Takeuchi, W.; Haryati, A.; M, R. Najib A.; Na'aim, M.

2016-06-01

The objective on this study is to investigate age and yield of FFB of oil palms based on ALOS PALSAR 2. Study areas in oil palm plantations areas of Jerantut, Pahang Malaysia. Methodology consists collecting of ALOS PALSAR 2 and tabular data on the study area, processing of ALOS PALSAR 2 including of converting digital numbers to normalize radar cross sections (NRCS), topography correction and filtering, making of regions of interest according to areas of age and yield of FFB of oil palms and making of relationship analysis between backscatter value of HH, HV and age and yield of FFB of oil palm. The results have showed relationship between HH, HV and age of oil palm which R2 of 0.63 for HH and 0.42 for HV that indicated increasing of age of oil palm as increasing of HH and HV value. Also relationship between HH, HV and yield of FFB of oil palm which R2 of 0.26 for HH and 0.15 for HV, that indicated increasing of yield of FFB as decreasing of HH and HV value.

Hypoglycemic and Hypolipidemic Effects of Aloe vera Extract Preparations: A Review.

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Pothuraju, Ramesh; Sharma, Raj Kumar; Onteru, Suneel Kumar; Singh, Satvinder; Hussain, Shaik Abdul

2016-02-01

Obesity is considered to be an epidemic disease, and it is associated with several metabolic disorders. Pharmacological treatments currently available are not effective for prolonged treatment duration. So, people are looking toward new therapeutic approach such as herbal ingredients. Since ancient periods, different herbs have been used for remedy purposes such as anti-obesity, antidiabetes, and antiinflammatory. Among the several herbal ingredients, Aloe vera (Aloe barbadensis Miller) is widely used to curb the metabolic complications. Till date, reports are not available for the side effects of A. vera. Several researchers are used to different solvents such as aqueous solution, alcohol, ethanol, and chloroform for the A. vera extract preparations and studied their hypoglycemic and hypolipidemic effects in animal and human studies. Furthermore, little information was recorded with the active compounds extracted from the A. vera and their anti-obesity and antidiabetic effects in clinical studies. In this review, we made an attempt to compile all the available literature by using different search engines (PubMed, Scopus, and Google Scholar) on the A. vera extract preparations and the possible mechanism of action involved in carbohydrate and lipid metabolism. Copyright © 2015 John Wiley & Sons, Ltd.

Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens

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Aloes have been widely used for a broad range of pharmacological activities, including parasitic problems. Avian coccidiosis is the most costly and wide-spread parasitic disease in the poultry industry, and has been mainly controlled by the use of chemotherapeutic agents. Due to the emergence of dru...

Mineral Content and Biochemical Variables of Aloe vera L. under Salt Stress

Science.gov (United States)

Murillo-Amador, Bernardo; Córdoba-Matson, Miguel Víctor; Villegas-Espinoza, Jorge Arnoldo; Hernández-Montiel, Luis Guillermo; Troyo-Diéguez, Enrique; García-Hernández, José Luis

2014-01-01

Despite the proven economic importance of Aloe vera, studies of saline stress and its effects on the biochemistry and mineral content in tissues of this plant are scarce. The objective of this study was to grow Aloe under NaCl stress of 0, 30, 60, 90 and 120 mM and compare: (1) proline, total protein, and enzyme phosphoenolpyruvate carboxylase (PEP-case) in chlorenchyma and parenchyma tissues, and (2) ion content (Na, K, Ca, Mg, Cl, Fe, P. N, Zn, B, Mn, and Cu) in roots, stems, leaves and sprouts. Proline and PEP-case increased as salinity increased in both parenchyma and chlorenchyma, while total protein increased in parenchyma and decreased in chlorenchyma, although at similar salt concentrations total protein was always higher in chlorenchyma. As salinity increased Na and Cl ions increased in roots, stems, leaves, while K decreased only significantly in sprouts. Salinity increases typically caused mineral content in tissue to decrease, or not change significantly. In roots, as salinity increased Mg decreased, while all other minerals failed to show a specific trend. In stems, the mineral concentrations that changed were Fe and P which increased with salinity while Cu decreased. In leaves, Mg, Mn, N, and B decreased with salinity, while Cu increased. In sprouts, the minerals that decreased with increasing salinity were Mg, Mn, and Cu. Zinc did not exhibit a trend in any of the tissues. The increase in protein, proline and PEP-case activity, as well as the absorption and accumulation of cations under moderate NaCl stress caused osmotic adjustment which kept the plant healthy. These results suggest that Aloe may be a viable crop for soil irrigated with hard water or affected by salinity at least at concentrations used in the present study. PMID:24736276

Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study.

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Bazvand, Leila; Aminozarbian, Mohammad Ghasem; Farhad, Alireza; Noormohammadi, Hamid; Hasheminia, Seyed Mohsen; Mobasherizadeh, Sina

2014-07-01

The aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, Propolis and Aloe vera on Enterococcus faecalis in deep dentin. Ninety fresh extracted single-rooted teeth were used in a dentin block model. Seventy-five teeth were infected with E. faecalis and divided into four experimental groups (n = 15). Experimental groups were treated with triantibiotic mixture with distilled water, 0.2% chlorhexidine gel, 70% ethanol + Propolis and Aloe vera. Fifteen teeth treated with distilled water as the positive control and 15 samples, free of bacterial contamination, were considered as the negative control. Gates-Glidden drill #4 was used for removal of surface dentin and Gates-Glidden drill #5 was used to collect samples of deep dentin. The samples were prepared and colony-forming units were counted. Data were analyzed by one-way ANOVA and post hoc Tukey tests. Statistical significance was defined at P 0.05). Aloe vera had antibacterial effects on E. faecalis, but in comparison with other medicaments, it was less effective (P vera were relatively effective against E. faecalis. All the intracanal medicements had similar effects on E. faecalis in deep dentin except for Aloe vera.

The influence of Aloe vera and xenograft XCB toward of bone morpho protein 2 BMP2 expression and amount of osteoblast of alveolar bone induced into tooth extraction sockets Cavia cobaya

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Utari Kresnoadi

2014-12-01

Full Text Available Tooth extraction can cause inflammation leading to alveolar ridge resorption. In addition, prominent ridge has crucial role for making denture su-ccessfully. Thus, socket preservation is needed to prevent greater alveolar ridge resorption. An innovative material, a combination of Aloe vera and xe-nograft (XCB, is then considered as a biogenic stimulator that can reduce inflammation, as a result, the growth of alveolar bone is expected to be impro-ved. This research is aimed to prove whether the mixture of Aloe vera and xenograft can stimulate BMP2 and increase osteoblasts. Forty-eight Cavia co-baya animals were divided into eight groups each of which consisted of six animals. The mandibular incisors of those Cavia cobaya animals were then extracted and filled with PEG as Group Control, XCB as Group XCB, Aloe vera as Group Aloe vera, and a combination of Aloe vera +XCB as Group Aloe vera +XCB. Next, the first four groups were sacrificed seven days after extraction, and the second four groups were sacrificed 30 days after extrac-tion. And then, immunohistochemical and histopathology examinations were conducted to examine BMP2 expression and osteoblasts. Based on the re-sult known that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblasts. It can be concluded that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblast cel . It can be used as an alternative material to increase the growth of alveolar bone after extraction.

Degradación de Fenantreno por bacterias del género Burkholderia y Rhizobium aisladas de nódulos de mimosas

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Arnoldo Wong-Villarreal

2017-01-01

Full Text Available El presente trabajo tuvo como objetivo identificar y evaluar la capacidad de degradación de microorganismos aislados de nódulos de mimosas, que puedan ser utilizados en procesos de biorremediación de suelos contaminados con fenantreno . Método . Se realizó el aislamiento de 122 cepas bacterianas de nódulos de mimosas; fueron crecidas en el medio de cultivo Maconkey para descartar enterobacterias. L as cepas bacterianas que dieron resultado negativo a esta prueba, fueron inoculadas en el medio de cultivo que contenía como úni ca fuente de carbono fenantreno; tres aislados tuvieron la capacidad de crecer en este medio. Las tres cepas fueron identificadas por secuencia del gen 1 6s ribosomal, se evaluó su capacidad de crecimiento en presencia de fenantreno mediante curvas de crecimiento microbiano; la capacidad para degradar fenantreno de las tres cepas fue cuantificada por cromatografía de gases acoplado a masas. Resultados . La s secuencias obtenidas del gen 16s ribosomal tienen relación genética con las especies de Burkholderia phenoliruptrix , Burkholderia phymatum y Rhizobium paknamense. El crecimiento microbiano de las tres cepas, suministradas con fenantreno, tuvieron un comp ortamiento similar al control , el cual contenía succinato como fuente de carbono. La cepa de Burkholderia sp. BB26 degradó 78.5 % , Burkholderia sp. BB24 68.5 % y Rhizobium sp. BY8 99%. Discusión . Los resultados de degradación de fenantreno por las cepas de Burkholderia sp. BB26 , Burkholderia sp. BB24 y Rhizobium sp. BY8 sugieren que las tres cepas tienen p otencial para utilizarse en procesos de biorremediación de suelos contaminados con fenantreno.

Antiviral activity of Aloe vera against herpes simplex virus type 2: An ...

African Journals Online (AJOL)

SERVER

2007-08-06

Aug 6, 2007 ... In this study we tested the antiviral activity of a crude hot glycerine extract of Aloe vera gel which was grown in Bushehr (Southwest of Iran) against HSV-2 replication in Vero cell line. The extract showed antiviral activity against HSV-2 not only before attachment and entry of virus to the Vero cells but also.

Virulence factors of non-O1 non-O139 Vibrio cholerae isolated in Córdoba, Argentina Factores de virulencia de Vibrio cholerae no-O1 no-O139 aislados en Córdoba, Argentina

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C. Bidinost

2004-12-01

Full Text Available V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains was studied in a rabbit ileal loop assay and their genetic profiles were compared by PFGE. The environmental strains varied in their virulence phenotype and showed no-clonal relationships. The clinical strains were highly enterotoxic, hemolytic, proteolytic and showed indistinguishable PFGE profiles, although they differed in their cytotoxic activity. This is the first description, using cell culture and “in vivo” studies, of the virulence properties of non-O1 non-O139 V. cholerae from Argentina.En este trabajo se analizó la presencia y expresión de genes de virulencia en V. cholerae no-O1 no-O139 de origen clínico y ambiental, aislados en Córdoba, Argentina. La mayoría de las cepas estudiadas contiene los genes toxR y hlyA, pero no ctxA, zot, ace, tcpA y stn. Se analizó la actividad hemolítica y citotóxica de estas cepas en los sobrenadantes de cultivo, así como su potencial enterotóxico en ensayos de asa ileal ligada de conejo. Además, los aislamientos fueron comparados por sus perfiles genéticos en PFGE. Las cepas del medio ambiente mostraron variación en su fenotipo de virulencia y no mostraron relación clonal. Las cepas clínicas fueron muy enterotóxicas, hemolíticas, proteolíticas y mostraron perfiles indistinguibles de PFGE, aunque mostraron diferencias en su actividad citotóxica. En este trabajo se describen por primera vez, utilizando ensayos de cultivo celular e “in vivo”, propiedades de virulencia de V. cholerae no-O1 no-O139 aislados en Argentina.

Clinical efficacy of new aloe vera- and myrrh-based oral mucoadhesive gels in the management of minor recurrent aphthous stomatitis: a randomized, double-blind, vehicle-controlled study.

Science.gov (United States)

Mansour, Ghada; Ouda, Soliman; Shaker, Ahmed; Abdallah, Hossam M

2014-07-01

To evaluate the clinical efficacy, and safety of newly customized natural oral mucoadhesive gels, containing either aloe vera or myrrh as active ingredients, in the management of minor recurrent aphthous stomatitis (MiRAS). Ninety subjects with MiRAS were recruited from Oral Medicine Clinic, at Faculty of Dentistry, King Abdulaziz University, Saudi Arabia, for this randomized, double-blind, placebo-controlled study. Two new natural gels, containing aloe vera and myrrh, were prepared in a concentration of (0.5% w/w), in addition to a plain mucoadhesive gel used as a placebo. Patients with fresh ulcers (aloe gel showed complete ulcer healing, 86.7%, and 80% of them revealed subsidence of erythema and exudation, respectively, especially at day 6 visit, whereas 76.7% of myrrh-treated patients revealed almost absence of pain at day 6. No side effects were encountered with the use of any of the three gels. The new formulated aloe- and myrrh-based gels proved to be effective in topical management of MiRAS. Aloe was superior in decreasing ulcer size, erythema, and exudation; whereas myrrh resulted in more pain reduction. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

ALGUNOS APORTES DEL ENFOQUE INCRUSTADO DE LAS CIENCIAS COGNITIVAS A LAS CIENCIAS DE LA EDUCACIÓN

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Maria Inês Silenzi

2012-05-01

Full Text Available Las Ciencias Cognitivas se constituyen como un espacio construido pordistintas disciplinas cuyos aportes provienen desde la neurociencias, laepistemología, la psicología, la filosofía, la antropología, y la lingüística,entre otras. Ahora bien, cada una de las disciplinas que configuran a lasCiencias Cognitivas intenta responder a la pregunta qué es la mente desdesu propio interés específico. Frente a esta misma intencionalidad, explicarla cognición, se postulan distintas respuestas, las cuales pueden ubicarse, demanera muy general, dentro del  enfoque clásico o dentro del enfoqueincrustado   de las Ciencias Cognitivas.Algunos conceptos como  enseñanza situada, aprendizaje situado,instrucción anclada, cognición situada   y cognición distribuida, entreotros, son producto de la combinación de algunos nuevos aportes teóricos,provenientes particularmente del enfoque incrustado de las CienciasCognitivas, con el ámbito educativo. Creemos necesario poner en cuestión siel análisis de estos conceptos, atendiendo a la relevancia del entorno queéstos promueven, dan cuenta, o no, del impacto teórico del enfoqueincrustado de las Ciencias Cognitivas dentro del ámbito educativo.En el presente trabajo proponemos la reflexión epistemológica sobre larelevancia del contexto en las prácticas educativas, considerando al enfoqueincrustado de las Ciencias Cognitivas como un andamiaje teórico, adecuadoy significativo para su análisis. Tal vez, a partir del renovado análisis teóricoque las Ciencias Cognitivas nos ofrecen, podemos reconsiderar nuestraspropuestas educativas en donde el aprendizaje se encuentradescontextualizado y aislado promoviendo, contrariamente, la interaccióndel alumno con su contexto inmediato.

Aloe vera Induced Biomimetic Assemblage of Nucleobase into Nanosized Particles

Science.gov (United States)

Chauhan, Arun; Zubair, Swaleha; Sherwani, Asif; Owais, Mohammad

2012-01-01

Aim Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, biomimetic (employing microorganism/plants) synthesis of metal and inorganic materials nano-particles has emerged as a simple and viable strategy. In the present study, we have extended biological synthesis of nano-particles to organic molecules, namely the anticancer agent 5-fluorouracil (5-FU), using Aloe vera leaf extract. Methodology The 5-FU nano- particles synthesized by using Aloe vera leaf extract were characterized by UV, FT-IR and fluorescence spectroscopic techniques. The size and shape of the synthesized nanoparticles were determined by TEM, while crystalline nature of 5-FU particles was established by X-ray diffraction study. The cytotoxic effects of 5-FU nanoparticles were assessed against HT-29 and Caco-2 (human adenocarcinoma colorectal) cell lines. Results Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines. Conclusion In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints. PMID:22403622

Tras las huella del silencio: Potosí, los incas y Toledo

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Tristan Platt

2010-12-01

Full Text Available ¿Por qué los señores aymaras de Charcas y el Inca Paullo, después de entregar las minas de plata de Porco a Hernando Pizarro en 1538, guardaron silencio sobre Potosí (distante a unas pocas leguas, que no se descubrió hasta abril de 1545? Reuniendo un mosaico de datos aparentemente inconexos, este artículo revisa las versiones corrientes del descubrimiento del cerro e interroga un curioso silencio presente en las fuentes. Reconstruyendo una red previamente desconocida de incas y españoles, se propone que, más que el hallazgo providencial de un yanacona aislado (la versión recibida, Potosí se manifestó como continuación de la política de “obediencia” subyacente en la entrega de Porco, ahora dirigida por el rival del Inca Paullo: el Inca Manco refugiado en Vilcabamba. Esta política de Manco buscaba apoyar al Rey y las Nuevas Leyes (1542 en contra de la amenaza mayor representada por Gonzalo Pizarro y los encomenderos. El artículo comenta, asimismo, la promoción toledana de la “leyenda providencial”, retomando en cambio la interpretación de Guaman Poma de la entrega de Potosí como una iniciativa de los incas, quienes lo habrían fundado bajo Inca Túpac Yupanqui. Esta versión concuerda con las últimas investigaciones geológicas y arqueológicas.

Enfermedad Diverticular del Colon

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Gonzalo López Escobar

1991-06-01

Full Text Available

Los divertículos del colon han sido reconocidos por varios observadores desde hace más de un siglo, pero en su mayor parte se trataba de casos aislados, hoy se la considera como la enfermedad del siglo XX, la de la era moderna y de los países industrializados y de avanzada tecnología (5,18,33.

Según el diccionario de la Real Academia Española (11, divertículo, del latín, diverticulum, quiere decir desviación de un camino; y desde el punto de vista anatómico, apéndice hueco y terminado en fondo de saco. (Gráfica No. 1.

Goligher (17 lo define como la “posada al borde del camino, probablemente un lugar, a menudo, de mala reputación”.

Historia

Según Hackford (18, el proceso fué descrito brevemente por Littre a comienzos del siglo XVIII; pero se le atribuye a Cruveilhier la primera descripción como proceso patológico en 1849, quien, además, mencionó: “encontramos, no rara vez, en el sigmoide, entre las bandas de fibras musculares longitudinales, una serie de pequeños tumores piriformes oscuros, que están formados por hernias de la mucosa a través de brechas en la capa muscular” (17.

Fleischman en 1815 hizo la primera observación de la enfermedad y empleó el término divertículo (45.

Rokitansky en 1.849, habló de una enfermedad adquirida y consideró que su causa consistía en la constipación (45.

Virchowen 1853 describió la perisigmoiditis (45.

En 1859 Sidney Jones informó de una fístula colo-vesical debida a diverticulitis (5,45.

Loomis en 1870 describe una peritonitis como resultante de una diverticulitis (45.

En 1877 Ball describió la anatomía patológica de la enfermedad y presentó dos casos de fístula colovesical debidas a diverticulitis (9. Cripps en 1.888 popularizó la colostomía de desviación como tratamiento para la fístula colovesical(18...

The Utilization of ALOS PALSAR Image to Estimate Natural Forest Biomass: Case Study at Bogani Nani Wartabone National Park (Pemanfaatan Citra ALOS PALSAR dalam Menduga Biomasa Hutan Alam: Studi Kasus di Taman Nasional Bogani Nani Wartabone

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Nurlita Indah Wahyuni

2014-12-01

Full Text Available The development of remote sensing technology makes it possible to utilize its data in many sectors including forestry. Remote sensing image has been used to map land cover and monitor deforestation. This paper presents utilization of ALOS PALSAR image to estimate and map aboveground biomass at natural forest of Bogani Nani Wartabone National Park especially SPTN II Doloduo and SPTN III Maelang. We used modeling method between biomass value from direct measurement and digital number of satellite image. There are two maps which present the distribution of biomass and carbon from ALOS PALSAR image with 50 m spatial resolution. These maps were built based on backscatter polarization of HH and HV bands. The maps indicate most research area dominated with biomass stock 0-5.000 ton/ha.

Polymeric Films Loaded with Vitamin E and Aloe vera for Topical Application in the Treatment of Burn Wounds

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Gabriela Garrastazu Pereira

2014-01-01

Full Text Available Burns are serious traumas related to skin damage, causing extreme pain and possibly death. Natural drugs such as Aloe vera and vitamin E have been demonstrated to be beneficial in formulations for wound healing. The aim of this work is to develop and evaluate polymeric films containing Aloe vera and vitamin E to treat wounds caused by burns. Polymeric films containing different quantities of sodium alginate and polyvinyl alcohol (PVA were characterized for their mechanical properties and drug release. The polymeric films, which were produced, were thin, flexible, resistant, and suitable for application on damaged skin, such as in burn wounds. Around 30% of vitamin E acetate was released from the polymeric films within 12 hours. The in vivo experiments with tape stripping indicated an effective accumulation in the stratum corneum when compared to a commercial cream containing the same quantity of vitamin E acetate. Vitamin E acetate was found in higher quantities in the deep layers of the stratum corneum when the film formulation was applied. The results obtained show that the bioadhesive films containing vitamin E acetate and Aloe vera could be an innovative therapeutic system for the treatment of burns.

Mechanical, Thermal, and Morphological Properties of Nanocomposites Based on Polyvinyl Alcohol and Cellulose Nanofiber from Aloe vera Rind

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Adel Ramezani Kakroodi

2014-01-01

Full Text Available This work was devoted to reinforcement of polyvinyl alcohol (PVA using cellulose nanofibers from Aloe vera rind. Nanofibers were isolated from Aloe vera rind in the form of an aqueous suspension using chemimechanical technique. Mechanical characterizations showed that incorporation of even small amounts of nanofibers (as low as 2% by weight had significant effects on both the modulus and strength of PVA. Tensile modulus and strength of PVA increased, 32 and 63%, respectively, after adding 2% of cellulose nanofiber from Aloe vera rind. Samples with higher concentrations of nanofibers also showed improved mechanical properties due to a high level of interfacial adhesion and also dispersion of fibers. The results showed that inclusion of nanofibers decreased deformability of PVA significantly. Dynamic mechanical analysis revealed that, at elevated temperatures, improvement of mechanical properties due to the presence of nanofibers was even more noticeable. Addition of nanofibers resulted in increased thermal stability of PVA in thermogravimetric analysis due to the reduction in mobility of matrix molecules. Morphological observations showed no signs of agglomeration of fibers even in composites with high cellulose nanofiber contents. Inclusion of nanofibers was shown to increase the density of composites.

Polymeric films loaded with vitamin E and aloe vera for topical application in the treatment of burn wounds.

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Pereira, Gabriela Garrastazu; Guterres, Sílvia Stanisçuaki; Balducci, Anna Giulia; Colombo, Paolo; Sonvico, Fabio

2014-01-01

Burns are serious traumas related to skin damage, causing extreme pain and possibly death. Natural drugs such as Aloe vera and vitamin E have been demonstrated to be beneficial in formulations for wound healing. The aim of this work is to develop and evaluate polymeric films containing Aloe vera and vitamin E to treat wounds caused by burns. Polymeric films containing different quantities of sodium alginate and polyvinyl alcohol (PVA) were characterized for their mechanical properties and drug release. The polymeric films, which were produced, were thin, flexible, resistant, and suitable for application on damaged skin, such as in burn wounds. Around 30% of vitamin E acetate was released from the polymeric films within 12 hours. The in vivo experiments with tape stripping indicated an effective accumulation in the stratum corneum when compared to a commercial cream containing the same quantity of vitamin E acetate. Vitamin E acetate was found in higher quantities in the deep layers of the stratum corneum when the film formulation was applied. The results obtained show that the bioadhesive films containing vitamin E acetate and Aloe vera could be an innovative therapeutic system for the treatment of burns.

Evaluation of wound healing treated with latex derived from rubber trees and Aloe Vera extract in rats.

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Brandão, Marcelo Luiz; Reis, Paulo Roberto Melo; Araújo, Lilhian Alves de; Araújo, Ana Carolina Vicente; Santos, Maisa Helena de Abreu Silva; Miguel, Marina Pacheco

2016-09-01

To compare the use of latex derivative and Aloe vera extract to wound healing. Twenty one rats were randomly divided into three groups and each one had a wound made by incision. The treatment consisted in: derivative of latex (GL), Aloe vera extract (GA) and saline solution (GC). The wound area was measured on the 7th, 14th and 21st days and macroscopic and microscopic evaluation were done. The comparison between the measurements of the wounds presented statistical difference in GC and GA from the 7th day of evaluation and GL from the 14th day. The extent of the wound was significantly smaller by the 7th day in GL. Histologically, in GL, the neovascularization was significant on the 7th, 14th and 21st days. On the 21st day the scar was large and little mature. In GA and GC, the findings were similar on the 7th, 14th and 21st days with a slight better organization of skin and collagen on the 21st in GA. Statistical analysis did not allow for the definition of the best topical agent. The latex had the highest angiogenesis, but a possible foreign body granuloma. Aloe vera has revealed a healing process adequated temporally in histology.

In vitro and biotransformational studies of aloe barbadensis mill

International Nuclear Information System (INIS)

Badar, Z.; Khan, S.; Ali, S.K.; Choudhary, M.I.

2014-01-01

Tissue culture technology can play an important role in the yield improvement of active ingredients of medicinal plants. In the present study, the potential of regeneration system of Aloe barbadensis along with biotransformational ability was explored. The maximum calli (5.65+-1.90; fresh weight) were induced under the dark condition on MS (Murashige and Skoog) medium supplemented with 2.0 mg/L of NAA (alpha-naphthaleneacetic acid), as compared to light. The highest number of shoots (12.725) were proliferated on MS regeneration medium, containing 1.0 mg/L of BAP (6-Benzyl Aminopurine) and 0.1 mg/L of IBA (Indole-3-Butyric Acid) incubated at 22 +- 2 degree C and 16/8 hr photoperiod provided by white fluorescent tube lights. These plantlets were then transferred onto root inducing medium and maximum number of roots (8.0 +- 0.70) with longer length (6.38 +- 0.34 cm) acquired at 1.0 mg/L of IBA within 14-20 days. The regenerated plants were shifted to green house for acclimatization. Effect of plant growth regulators and light was also assessed on callus cultures produced from conventionally propagated and in-vitro regenerated A. barbadensis plants. Biotransformation ability of Aloe barbadensis cell suspension culture was studied by incubation with (+)-adrenosterone (1), which afforded three products; D1-2-dehydroadrenosterone (2), 5a-androst-1-ene-3, 11, 17-trione (3) and 17b-hydroxyandrost-4-ene-3, 11-dione (4). These metabolites were structurally characterized on the basis of spectroscopic techniques. (author)

The effectiveness of Aloe vera barbadensis bioactives on laying hens on commercial farmers

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Tiurma Pasaribu

2006-06-01

Full Text Available A field trial was conducted to study the effectiveness of dry gel Aloe vera (DG as a feed additive for laying hens in commercial farms. The trial was consisted of two treatments, one was control, commonly used farmer ration containing antibiotic of zinc bacitracin at 0.5 g/kg and the second feed containing DG equal to 1.0 g/kg. Every treatment had two replicates with 504 Loghman laying hens. The hen day production (% HD, egg weight, feed consumption, feed conversion ratio (FCR, egg quality comprising yolk colour, albumin and yolk weights, egg shell eight and thikness, and mortality were observed for 24 weeks. The results showed that feed consumption, % HD, egg weight, FCR, yolk colour, albumin weight, yolk weight, egg shell weight, and egg shell thickness were not significantly different (P>0.05 between the control and DG treatment, except for the Haugh unit (HU. Thus, it can be concluded that Aloe vera bioactives has the same effectiveness as antibiotic as a feed additive at the level of commercial farms.

Presencia de agentes potenciales causantes de infecciones subcutáneas humanas en suelo y plantas en el estado de Puebla, México

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Alejandra P. Espinosa Texis

2017-04-01

Full Text Available Los hongos y actinomicetos asociados a infecciones en humanos, se encuentran ampliamente distribuidos en la naturaleza. El suelo y las plantas son el hábitat de numerosos hongos y bacterias. Los campesinos son altamente vulnerables a sufrir heridas y pueden contaminarse con estos microorganismos. Con la finalidad de conocer el número de colonias de los agentes causantes de micosis subcutáneas humanas a partir de la naturaleza, se realizó el aislamiento de los microorganismos respectivos a partir de suelo y plantas en 11 municipios del estado de Puebla. De cada municipio se colectaron 50 muestras, cada una consistió de 10 g de suelo y 10 g de hojas de la planta más cercana; se prepararon suspensiones respectivas, las cuales fueron inoculadas en agar dextrosa Sabouraud, incubadas a 28 ºC, y revisadas periódicamente para identificar los microorganismos de interés con base en su morfología. De 110 muestras procesadas, se obtuvieron 441 aislados, de los cuales 281 fueron hongos (133 de suelo y 148 de plantas y 160 actinomicetos (96 de suelo y 64 plantas. Los hongos aislados fueron identificados por su morfología macroscópica y microscópica. Los actinomicetos fueron identificados por su morfología macroscópica (colonial y microscópica, y por pruebas bioquímicas. En mayor proporción se aislaron agentes potenciales de cromoblastomicosis (Fonsecaea pedrosoi y Cladophialophora carrionii, seguidos del agente de esporotricosis (Sporothrix schenckii y de los agentes de actinomicetoma (Nocardia brasiliensis y N. otitidis-caviarum. Del suelo de Cholula y de plantas de Tecali de Herrera, se obtuvieron el mayor número de aislados fúngicos. Del suelo de Chignahuapan y de plantas de Izúcar de Matamoros se obtuvo el mayor número de aislados de actinomicetos. En este estudio se encontraron, tanto en suelo como en plantas de 11 municipios del estado de Puebla, un alto número de colonias de hongos y actinomicetos causantes de infecciones subcut

Comparison of the antibacterial effect of sodium hypochlorite and aloe vera solutions as root canal irrigants in human extracted teeth contaminated with enterococcus faecalis.

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Sahebi, S; Khosravifar, N; Sedighshamsi, M; Motamedifar, M

2014-03-01

The main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. Sodium hypochlorite has excellent antibacterial properties, but also some negative features. The aim of the present study is to compare the antimicrobial effect of Aloe Vera solution with sodium hypochlorite on E.faecalis in the root canals of human extracted teeth. Sixty human extracted single rooted teeth were selected for this in vitro study. The teeth recruited in this study had no cracks, internal resorption, external resorption and calcification. Enterococcus faecalis was injected in the root canals of all teeth. The teeth were then divided into three groups randomly. Each group consisted of 20 teeth that were all rinsed with one of the following solutions: sodium hypochlorite 2.5%, Aloe vera and normal saline. Subsequent to rinsing, root canals of all teeth were sampled. The samples were cultured and growth of the bacteria was assessed after 48 hours. The number of colonies of the bacteria was then counted. The difference between the inhibitory effect of Aloe vera and normal saline on E.faecalis was not significant according to independent t-test (p= 0.966). The inhibitory effect of sodium hypochlorite on E.faecalis was much greater than that of Aloe vera and normal saline (pvera solution is not recommended as a root canal irrigator, but future studies are suggested to investigate the antibacterial effect of Aloe vera with longer duration of exposure and as an intra canal medicament.

Quinta parte. Pudriciones Fungosas Radiculares del Fríjol en Valle de Medellín Colombia

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Cárdona Álvarez Canúto

1954-11-01

Full Text Available En esta parte se hace el resumen del estudio que se realizo sobre las pudriciones fungosas radiculares del fríjol en el valle de Medellín, a continuación se enumeran las conclusiones a las que se llegaron: 1A partir de 1948 se hizo un estudio de determinación de los hongos que causan pudriciones de la raíz del fríjol. Dicho estudio se efectuó en Medellín e incluyó organismos de la región y de otros lugares, como Andes, Armero y Palmira. Se usaron organismos que se encontraron asociados con pudriciones de la raíz del fríjol, vigna, remolacha y soya. 2Lo organismos aislados se obtuvieron directamente de las raíces afectadas y no de otra fuente como el suelo o la semilla. 3Concomitante con la obtención de los cultivos de los organismos, se hizo un estudio abarcando apreciación de distribución de los hongos en los plantíos y pérdidas en rendimiento, causadas por los agentes, expresadas en peso de semilla. Se obtuvieron porcentajes naturales de infección variando entre 68.55 y 100% y reducción en el rendimiento hasta del 93%. 4Se hizo una descripción de síntomas y de signos para cada especie patógena encontrada durante este trabajo. Asimismo se estudió la clasificación taxonómica de cada organismo en particular.

Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia

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Robinson Pacheco

2014-04-01

Full Text Available Introducción. Las enzimas carbapenemasas de tipo KPC tienen gran capacidad de diseminación, son causantes de epidemias y se asocian a mayor mortalidad y estancia hospitalaria. En Colombia se han venido reportando cada vez más desde 2007, pero se desconoce la prevalencia hospitalaria. Objetivo. Estimar la prevalencia hospitalaria del gen blaKPC. Materiales y métodos. Se evaluó la presencia del gen blaKPC y su ‘clonalidad’ en aislamientos de enterobacterias y Pseudomonas aeruginosa de pacientes hospitalizados. Resultados. De los 424 aislamientos evaluados durante el periodo de estudio, 273 cumplieron con criterios de elegibilidad, 31,1 % fue positivo para el gen blaKPC y, al ajustar por ‘clonalidad’, la positividad fue de 12,8 %. El gen blaKPC se encontró con mayor frecuencia en Klebsiella pneumoniae seguido de P. aeruginosa y otras enterobacterias. A pesar de que la unidad de cuidados intensivos aportó el mayor número de aislamientos, no se encontró un patrón más prevalente del gen blaKPC en las ellas que en las otras salas. El aparato respiratorio fue el sitio anatómico de origen con la mayor prevalencia. No se presentó estacionalidad en la frecuencia de los aislamientos portadores del gen blaKPC. Conclusión. Este estudio reveló la alta prevalencia del gen blaKPC en diferentes microorganismos aislados en varias instituciones hospitalarias del país. La extraordinaria capacidad de propagación del gen blaKPC, las dificultades del diagnóstico y la limitada disponibilidad de antibióticos plantean la apremiante necesidad de fortalecer los sistemas de vigilancia epidemiológica y ajustar oportunamente las políticas institucionales de uso racional de antibióticos con el fin de contener su diseminación a otras instituciones de salud del país.

Forest Disturbances and Regrowth Assessment Using ALOS PALSAR Data from 2007 to 2010 in Vietnam, Cambodia and Lao PDR

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Stéphane Mermoz

2016-03-01

Full Text Available This paper aims to develop a new methodology for monitoring forest disturbances and regrowth using ALOS PALSAR data in tropical regions. In the study, forest disturbances and regrowth were assessed between 2007 and 2010 in Vietnam, Cambodia and Lao People’s Democratic Republic. The deforestation rate in Vietnam has been among the highest in the tropics in the last few decades, and those in Cambodia and Lao are increasing rapidly. L-band ALOS PALSAR mosaic data were used for the detection of forest disturbances and regrowth, because L-band SAR intensities are sensitive to forest aboveground biomass loss. The methodology used here combines SAR data processing, which is particularly suited for change detection, forest detection and forest disturbances and regrowth detection using expectation maximization, which is closely related to fuzzy logic. A reliable training and testing database has been derived using AVNIR-2 and Google Earth images for calibration and validation. Efforts were made to apply masking areas that are likely to show different SAR backscatter temporal behaviors from the forests considered in the study, including mangroves, inundated forests, post-flooding or irrigated croplands and water bodies, as well as sloping areas and urban areas. The resulting forest disturbances and regrowth map (25-m resolution indicates disturbance rates of −1.07% in Vietnam, −1.22% in Cambodia and −0.94% in Lao between 2007 and 2010, with corresponding aboveground biomass losses of 60.7 Tg, 59.2 Tg and 83.8 Tg , respectively. It is expected that the method, relying on free of charge data (ALOS and ALOS2 mosaics, can be applied widely in the tropics.

Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study

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Leila Bazvand

2014-01-01

Full Text Available Background: The aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, Propolis and Aloe vera on Enterococcus faecalis in deep dentin. Materials and Methods: Ninety fresh extracted single-rooted teeth were used in a dentin block model. Seventy-five teeth were infected with E. faecalis and divided into four experimental groups (n = 15. Experimental groups were treated with triantibiotic mixture with distilled water, 0.2% chlorhexidine gel, 70% ethanol + Propolis and Aloe vera. Fifteen teeth treated with distilled water as the positive control and 15 samples, free of bacterial contamination, were considered as the negative control. Gates-Glidden drill #4 was used for removal of surface dentin and Gates-Glidden drill #5 was used to collect samples of deep dentin. The samples were prepared and colony-forming units were counted. Data were analyzed by one-way ANOVA and post hoc Tukey tests. Statistical significance was defined at P 0.05. Aloe vera had antibacterial effects on E. faecalis, but in comparison with other medicaments, it was less effective (P < 0.05. Conclusion: This experimental study showed that triantibiotic mixture, 0.2% chlorhexidine gel, Propolis and Aleo vera were relatively effective against E. faecalis. All the intracanal medicements had similar effects on E. faecalis in deep dentin except for Aloe vera.

Capacity of the terrestrial entomopathogenic nematode Steinernema rarum (Rhabditida: Steinernematidae to parasite Culex apicinus larvae (Diptera: Culicidae Capacidad del nemátodo terrestre entomopatógeno Steinernema rarum (Rhabditida: Steinernematidae de parasitar larvas de Culex apicinus (Diptera: Culicidae

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Susana R. Cagnolo

2010-06-01

Full Text Available Entomopathogenic nematodes can be considered effective agents for biocontrol, resulting innocuous for humans. Larvaeof Culex apicinus Philippi were exposed to infective juveniles of Steinernema rarum (OLI strain under laboratory conditions, testing six doses (1:1, 5:1, 10:1, 15:1, 100:1, 400:1. An increasing percentage of mosquito larvae mortality was recorded with an increased dose. The highest percentage of mosquito larvae mortality (75% was obtained with the dose 400:1. This is the first report of parasitism of an isolated of S. rarum from Córdoba against larvae of C. apicinus, with promising results. Therefore, further studies must be carried out to determine if these nematodes would be effective as autochthonous agents for the control of Culex Linnaeus and other mosquitoes of sanitary interest in the country.Los nemátodos entomopatógenos son considerados eficientes agentes de control de insectos plaga e inocuos para los humanos. Larvas de Culex apicinus Philippi fueron expuestas a seis dosis (1:1, 5:1, 10:1, 15:1, 100:1, 400:1 de juveniles infectivos de Steinernema rarum (aislado OLI. Se registró un incremento en la mortalidad de las larvas del mosquito con el aumento de la dosis del nematodo. El mayor porcentaje de mortalidad de larvas del mosquito (75% se obtuvo con la dosis 400:1. Este es el primer reporte de parasitismo de un aislado de S. rarum de Córdoba, en larvas de C. apicinus con resultados promisorios. Por lo tanto, se debería profundizar su estudio para determinar si pueden resultar efectivos como agentes autóctonos para el control biológico de mosquitos Culex Linnaeus, y otros de interés sanitario en el país.

Improvement of multi-level resistive switching characteristics in solution-processed AlO x -based non-volatile resistive memory using microwave irradiation

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Kim, Seung-Tae; Cho, Won-Ju

2018-01-01

We fabricated a resistive random access memory (ReRAM) device on a Ti/AlO x /Pt structure with solution-processed AlO x switching layer using microwave irradiation (MWI), and demonstrated multi-level cell (MLC) operation. To investigate the effect of MWI power on the MLC characteristics, post-deposition annealing was performed at 600-3000 W after AlO x switching layer deposition, and the MLC operation was compared with as-deposited (as-dep) and conventional thermally annealing (CTA) treated devices. All solution-processed AlO x -based ReRAM devices exhibited bipolar resistive switching (BRS) behavior. We found that these devices have four-resistance states (2 bits) of MLC operation according to the modulation of the high-resistance state (HRSs) through reset voltage control. Particularly, compared to the as-dep and CTA ReRAM devices, the MWI-treated ReRAM devices showed a significant increase in the memory window and stable endurance for multi-level operation. Moreover, as the MWI power increased, excellent MLC characteristics were exhibited because the resistance ratio between each resistance state was increased. In addition, it exhibited reliable retention characteristics without deterioration at 25 °C and 85 °C for 10 000 s. Finally, the relationship between the chemical characteristics of the solution-processed AlO x switching layer and BRS-based multi-level operation according to the annealing method and MWI power was investigated using x-ray photoelectron spectroscopy.

Physico-chemical evaluation of bitter and non-bitter Aloe and their raw juice for human consumption.

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Azam, M M; Kumar, S; Pancholy, A; Patidar, M

2014-11-01

In addition to Aloe vera which is bitter in taste, a non-bitter Aloe is also found in arid part of Rajasthan. This non-bitter Aloe (NBA) is sporadically cultivated as vegetable and for health drink. In spite of its cultivation and various uses, very little information is available about its detailed botanical parameters and chemical characters. This study aims to evaluate the physico-chemical characters of NBA through employing floral morphology, leaf characters and leaf gel and to compare them with those of A. vera. Of eleven floral characters studied, eight characters of NBA were significantly different from that of A. vera. Most visible difference was observed in their reproductive shoots which are highly branched in NBA (5.21 inflorescence/shoot) as compared to A. vera (1.5 inflorescence/shoot). NBA produces less leaf-biomass (-29.32 %) with less leaf-thickness (-31.44 %) but higher leaf length, width, and no. of spine/side by 17.56 %, 21.34 % and 16.11 %, respectively, with significant difference as compared to A. vera. But its polysaccharide content (0.259 %) is at par with that of A. vera. The raw juice from the leaf of NBA has very low aloin content (4.1 ppm) compared to that from A. vera (427.3 ppm) making it a safer health drink compared to the one obtained from A. vera. Thus, NBA raw juice emerged as suitable alternative to A. vera juice for human consumption.

Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel

Energy Technology Data Exchange (ETDEWEB)

Grindlay, D.; Reynolds, T.

1986-06-01

The mucilaginous gel from the parenchymatous cells in the leaf pulp of Aloe vera has been used since early times for a host of curative purposes. This gel should be distinguished clearly from the bitter yellow exudate originating from the bundle sheath cells, which is used for its purgative effects. Aloe vera gel has come to play a prominent role as a contemporary folk remedy, and numerous optimistic, and in some cases extravagant, claims have been made for its medicinal properties. Modern clinical use of the gel began in the 1930s, with reports of successful treatment of X-ray and radium burns, which led to further experimental studies using laboratory animals in the following decades. The reports of these experiments and the numerous favourable case histories did not give conclusive evidence, since although positive results were usually described, much of the work suffered from poor experimental design and insufficiently large test samples. In addition some conflicting or inconsistent results were obtained. With the recent resurgence of interest in Aloe vera gel, however, new experimental work has indicated the possibility of distinct physiological effects. Chemical analysis has shown the gel to contain various carbohydrate polymers, notably either glucomannans or pectic acid, along with a range of other organic and inorganic components. Although many physiological properties of the gel have been described, there is no certain correlation between these and the identified gel components. 154 references.

Effects of gamma irradiation and ozone treatment on microbial decontamination and fatty acid compositions of aloe and pollen powders

International Nuclear Information System (INIS)

Yook, H.S.; Chung, Y.J.; Kim, J.O.; Kwon, O.J.; Byun, M.W.

1997-01-01

The comparative effects of gamma irradiation and ozone treatment on the microbial inactivation and fatty acid composition were investigated for improving hygienic quality of aloe and pollen powders. Gamma irradiation at 10 kGy resulted in sterilizing total aerobic bacteria, molds and coliforms below detective levels, while ozone treatment for 8 hours up to 18 ppm did not sufficiently eliminate the microorganisms of aloe and pollen powders. The compositions of fatty acid were not significantly changed by gamma irradiation up to 10 kGy. However, ozone treatment markedly decreased unsaturated fatty acids by approximately 20-80% in contents, whereas it significantly increased saturated fatty acids (p<0.05)

Effect of Aloe vera, chlorine dioxide, and chlorhexidine mouth rinses on plaque and gingivitis: A randomized controlled trial

Science.gov (United States)

Yeturu, Sravan Kumar; Acharya, Shashidhar; Urala, Arun Sreenivas; Pentapati, Kalyana Chakravarthy

2015-01-01

Objective To evaluate the effect of Aloe vera, chlorine dioxide, and chlorhexidine mouth rinses on plaque and gingivitis in orthodontic treatment. Materials and methods A randomized single-center, single-blind, parallel group, controlled trial was conducted among 90 subjects undergoing fixed orthodontic treatment. The subjects were randomly divided into one of the three study groups (Aloe vera, chlorhexidine, chlorine dioxide). Plaque and gingivitis were assessed using modified Silness and Loe Plaque Index and Gingival Index at baseline and at follow-up after 15 days. Paired t-test and ANOVA with post hoc Dunnett test were used. A p-value of <0.05 was considered statistically significant. Results A total of 85 participants completed the study; among them, 40 were male and 45 were female. There was significant reduction in mean plaque and gingival scores in all the 3 groups at follow-up when compared to baseline. A significantly higher reduction (plaque and gingival scores) was found in chlorhexidine when compared with the Aloe vera group. However, no significant difference was seen between chlorhexidine and chlorine dioxide with respect to mean reduction in plaque and gingival scores. Conclusion Chlorine dioxide can be a suitable and economical alternative for chlorhexidine. Further long-term studies are recommended for evaluating their effectiveness. PMID:26937371

Effects of plant sterols derived from Aloe vera gel on human dermal fibroblasts in vitro and on skin condition in Japanese women

OpenAIRE

Tanaka, Miyuki; Misawa,Eriko; Yamauchi,Koji; Abe,Fumiaki; Ishizaki,Chiaki

2015-01-01

Miyuki Tanaka,1 Eriko Misawa,1 Koji Yamauchi,1 Fumiaki Abe,1 Chiaki Ishizaki2 1Functional Food Research Department, Food Science and Technology Institute, Morinaga Milk Industry Co, Ltd, Zama, Kanagawa, 2Ebisu Skin Research Center, Inforward, Inc., Tokyo, Japan Background: Aloe is known for its topical use for treating wounds and burns. Many previous studies reported the healing effects of Aloe vera. However, there are few clinical studies on the effect of orally administered A. vera gel on ...

Cirugía de reemplazo valvular aórtico aislado en pacientes octogenarios: evaluación, riesgo operatorio y resultados a mediano plazo

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Fernando Piccinini

2010-01-01

Full Text Available RESUMENIntroducciónLas indicaciones y los beneficios del reemplazo valvular aórtico han sido claramente establecidos, aun para poblaciones añosas, de aumentada comorbilidad. Aun así, la manipulación de ateromas aórticos, el uso de circulación extracorpórea y de clampeo aórtico, el paro cardíaco y la descalcificación ligadas a esta técnica y sus eventuales consecuencias generan dudas acerca de su indicación en este grupo de pacientes.ObjetivosComunicar la morbimortalidad del procedimiento en octogenarios y validar la utilidad de los puntajes de predicción de riesgo utilizados más frecuentemente.Material y métodosSe estudiaron 87 pacientes octogenarios sometidos a reemplazo aórtico aislado; se analizaron variables preoperatorias, intraoperatorias y posoperatorias y se aplicó el Euroscore; para facilitar el subanálisis del Euroscore logístico, la población se dividió en tres grupos: riesgo bajo, moderado y alto. Se utilizó técnica quirúrgica convencional. Para el seguimiento, se analizó la historia clínica y/o se realizó encuesta telefónica.ResultadosLa mediana de edad fue de 83 ± 2,5 años, con un rango de 80 a 89 años. El 60% de la población en estudio era de sexo femenino. La indicación quirúrgica fue estenosis 92%, endocarditis activa 4,6% y enfermedad valvular 3,4%. Antecedentes: hipertensión 71%, tabaquismo 31%, dislipidemia 39%, diabetes 11,5%, EPOC 10%, IRC-diálisis 2,3%, ACV 11,5%, IAM previo 8%, fibrilación auricular 16%, reoperación 15%. Cuadro clínico: asintomático 3,45%, angina crónica estable 10,3%, angina inestable 11%, ICC 13,8%, disnea CF III-IV 60%. Disfunción ventricular izquierda moderada-grave 18,3%. Riesgo por Euroscore logístico 12,4% ± 15%. El tiempo operatorio promedio fue de 200 ± 61,7 min, el de CEC de 86 ± 32,5 min y el de clampeo aórtico de 65 ± 18,2 min. Complicaciones: sangrado médico 17,2%, reoperación sangrado 5,7%, bajo gasto cardíaco 13,8%, inotrópicos > 48 horas

Beneficial effects of aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes.

Science.gov (United States)

Rajasekaran, Subbiah; Ravi, Kasiappan; Sivagnanam, Karuran; Subramanian, Sorimuthu

2006-03-01

The effect of diabetes mellitus on lipid metabolism is well established. The association of hyperglycaemia with an alteration of lipid parameters presents a major risk for cardiovascular complications in diabetes. Many secondary plant metabolites have been reported to possess lipid-lowering properties. The present study was designed to examine the potential anti-hyperlipidaemic efficacy of the ethanolic extract from Aloe vera leaf gel in streptozotocin (STZ)-induced diabetic rats. 2. Oral administration of Aloe vera gel extract at a dose of 300 mg/kg bodyweight per day to STZ-induced diabetic rats for a period of 21 days resulted in a significant reduction in fasting blood glucose, hepatic transaminases (aspartate aminotransferase and alanine aminotransferase), plasma and tissue (liver and kidney) cholesterol, triglycerides, free fatty acids and phospholipids and a significant improvement in plasma insulin. 3. In addition, the decreased plasma levels of high-density lipoprotein-cholesterol and increased plasma levels of low-density lipoprotein-and very low-density lipoprotein-cholesterol in diabetic rats were restored to near normal levels following treatment with the extract. 4. The fatty acid composition of the liver and kidney was analysed by gas chromatography. The altered fatty acid composition in the liver and kidney of diabetic rats was restored following treatment with the extract. 5. Thus, the results of the present study provide a scientific rationale for the use of Aloe vera as an antidiabetic agent.

Green synthesis and characterization of silver nanoparticle using Aloe barbadensis

Energy Technology Data Exchange (ETDEWEB)

Thappily, Praveen, E-mail: pravvmon@gmail.com, E-mail: shiiuvenus@gmail.com; Shiju, K., E-mail: pravvmon@gmail.com, E-mail: shiiuvenus@gmail.com [Laboratory for Molecular Photonics and Electronics (LAMP), Department of Physics, National Institute of Technology, Calicut, Kerala 673601 (India)

2014-10-15

Green synthesis of silver nanoparticles was achieved by simple visible light irradiation using aloe barbadensis leaf extract as reducing agent. UV-Vis spectroscopic analysis was used for confirmation of the successful formation of nanoparticles. Investigated the effect of light irradiation time on the light absorption of the nanoparticles. It is observed that upto 25 minutes of light irradiation, the absorption is linearly increasing with time and after that it becomes saturated. Finally, theoretically fitted the time-absorption graph and modeled a relation between them with the help of simulation software.

Green synthesis and characterization of silver nanoparticle using Aloe barbadensis

International Nuclear Information System (INIS)

Thappily, Praveen; Shiju, K.

2014-01-01

Green synthesis of silver nanoparticles was achieved by simple visible light irradiation using aloe barbadensis leaf extract as reducing agent. UV-Vis spectroscopic analysis was used for confirmation of the successful formation of nanoparticles. Investigated the effect of light irradiation time on the light absorption of the nanoparticles. It is observed that upto 25 minutes of light irradiation, the absorption is linearly increasing with time and after that it becomes saturated. Finally, theoretically fitted the time-absorption graph and modeled a relation between them with the help of simulation software

Estudios etiológicos de la marchitez del aguacate en Antioquia-Colombia

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Joaquín Guillermo Ramírez Gil

2014-02-01

Full Text Available El cultivo de aguacate en Colombia ha presentado un incremento en el área sembrada durante la última década, especialmente en clima frío moderado, entre 1.800 y 2.500 msnm. La variedad Hass se destaca por tener la mayor área sembrada en este clima, en el departamento de Antioquia-Colombia. Este cultivo presenta grandes retos tecnológicos para su expansión; entre ellos el manejo de enfermedades ocupa un lugar predominante por las implicaciones que tiene en costos, impacto en la salud, ambiente y restricciones para exportaciones. La marchitez, ocasiona la muerte de numerosos árboles en todas las etapas del cultivo y presenta la mayor incidencia y severidad de las enfermedades identificadas para este cultivo. El desconocimiento de los agentes causales de esta enfermedad, ha llevado a realizar prácticas de manejo encaminadas principalmente al control del oomycete Phytophthora cinnamomi Rands, como único agente causal. Esta investigación tuvo como objetivo identificar los distintos agentes causales de la marchitez del aguacate y la relación de su incidencia con las prácticas de manejo en lotes cultivados en el departamento de Antioquia-Colombia. Los resultados encontrados muestran que la marchitez es el principal problema del cultivo de aguacate. Los microorganismos P. cinnamomi, Phytophthora heveae Thompson, Phytophthora citrícola Sawada, Verticillium sp., y Cylindrocarpon destructans (Zinss Scholten fueron aislados y reprodujeron los síntomas asociados a marchitez. Las condiciones de bajo contenido de oxigeno el suelo también reprodujeron la sintomatología de marchitez, involucrando también un origen abiótico en la etiología.

Serotipificación de aislamientos clínicos y del medio ambiente de Cryptococcus neoformans en Colombia

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N. Ordóñez

1994-09-01

Full Text Available Se determinó l variedad y el serotipo de 192 aislamientos de Cryptococcus neoformans recuperados de 1972 a 1992; 163 de origen clínico y 29 del medio ambiente. En los aislamientos clínicos se determinaron las dos variedades de C. neoformans: la variedad neoformans en el 92,6% de los pacientes y la variedad gattii en el 7,4%. En el medio ambiente se encontró la variedad neoformans en el 100% de los aislamientos. El serotipo encontrado con más frecuencia fue el serotipo A, el cual se observó en el 92% de los aislamientos clínicos y en el 100% del medio ambiente, seguido por el serotipo B (6,8%; también, se determinaron aislamientos de los serotipos C (0,6% y D (0,6%. C. neoformans var. neoformans se recuperó en todos los años de los que se tuvieron aislamientos y C. neoformans var. gattii solamente se observó en aislamientos clínicos a partir de 1986. C. neoformans var. neoformans fue aislado durante los 12 meses del año y C. neoformans var. gattii se encontró distribuido en los meses defebrero, abril, junio, agosto, septiembre y diciembre. C. neoformans var. neoformans se observó en 109 (66,9% cepas de pacientes del sexo masculino y en 36 (22,1% del sexo femenino; C. neoformans var. gattii se observó en 5 (3% cepas de hombres y en 6 (3,7% mujeres.

Use of dexpanthenol and aloe vera to influence the irradiation response of the oral mucous membrane (mouse); Beeinflussung der Strahlenreaktion der Mundschleimhaut (Maus) durch Dexpanthenol {+-} Aloe vera

Energy Technology Data Exchange (ETDEWEB)

Schlichting, S. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany); Spekl, K.; Doerr, W. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany)]|[Experimentelles Zentrum, Medizinische Fakultaet Carl Gustav Carus, Dresden Univ. (Germany)

2004-07-01

In summarising the outcome of the present study it can be said spraying the agent on the oral mucous membrane once a day had an effect on the incidence of mucous membrane ulceration in the case of both placebo and dexpanthenol treatment. However, there was no significant difference between placebo and dexpanthenol treatment, the only finding being a slight prolongation of latency time through aloe vera. These experimental findings give good reason to critically reconsider the clinical use of dexpanthenol as a supportive treatment for the prevention of radiogenic mucositis enoralis following irradiation of tumours in the head and neck region. However thorough oral lavage is an effective means of moderating the irradiation response of the oral mucous membrane. [German] Zusammenfassend ist festzustellen, dass in den vorliegenden Versuchen mit einmal taeglichem Aufspruehen des Praeparates auf die Mundschleimhaut sowohl die Placebo - wie auch die Dexpanthenol-Behandlung die Inzidenz von Schleimhautulzerationen modifiziert hat; zwischen Placebo- und Dexpanthenol-Behandlung ergab sich jedoch kein signifikanter Unterschied. Lediglich eine geringe Verlaengerung der Latenzzeit durch Aloe vera war zu beobachten. Auf der Basis dieser experimentellen Ergebnisse muss der klinische Einsatz von Dexpanthenol im Rahmen der Supportivtherapie zur Prophylaxe der radiogenen Mucositis enoralis bei der Bestrahlung von Kopf-Hals-Tumoren kritisch ueberdacht werden. Regelmaessige, intensive Mundspuelungen sind jedoch ein probates Mittel zur Verminderung der Strahlenreaktion der Mundschleimhaut. (orig.)

Continental Scale Vegetation Structure Mapping Using Field Calibrated Landsat, ALOS Palsar And GLAS ICESat

Science.gov (United States)

Scarth, P.; Phinn, S. R.; Armston, J.; Lucas, R.

2015-12-01

Vertical plant profiles are important descriptors of canopy structure and are used to inform models of biomass, biodiversity and fire risk. In Australia, an approach has been developed to produce large area maps of vertical plant profiles by extrapolating waveform lidar estimates of vertical plant profiles from ICESat/GLAS using large area segmentation of ALOS PALSAR and Landsat satellite image products. The main assumption of this approach is that the vegetation height profiles are consistent across the segments defined from ALOS PALSAR and Landsat image products. More than 1500 field sites were used to develop an index of fractional cover using Landsat data. A time series of the green fraction was used to calculate the persistent green fraction continuously across the landscape. This was fused with ALOS PALSAR L-band Fine Beam Dual polarisation 25m data and used to segment the Australian landscapes. K-means clustering then grouped the segments with similar cover and backscatter into approximately 1000 clusters. Where GLAS-ICESat footprints intersected these clusters, canopy profiles were extracted and aggregated to produce a mean vertical vegetation profile for each cluster that was used to derive mean canopy and understorey height, depth and density. Due to the large number of returns, these retrievals are near continuous across the landscape, enabling them to be used for inventory and modelling applications. To validate this product, a radiative transfer model was adapted to map directional gap probability from airborne waveform lidar datasets to retrieve vertical plant profiles Comparison over several test sites show excellent agreement and work is underway to extend the analysis to improve national biomass mapping. The integration of the three datasets provide options for future operational monitoring of structure and AGB across large areas for quantifying carbon dynamics, structural change and biodiversity.

Aloe vera Derived Activated High-Surface-Area Carbon for Flexible and High-Energy Supercapacitors.

Science.gov (United States)

Karnan, M; Subramani, K; Sudhan, N; Ilayaraja, N; Sathish, M

2016-12-28

Materials which possess high specific capacitance in device configuration with low cost are essential for viable application in supercapacitors. Herein, a flexible high-energy supercapacitor device was fabricated using porous activated high-surface-area carbon derived from aloe leaf (Aloe vera) as a precursor. The A. vera derived activated carbon showed mesoporous nature with high specific surface area of ∼1890 m 2 /g. A high specific capacitance of 410 and 306 F/g was achieved in three-electrode and symmetric two-electrode system configurations in aqueous electrolyte, respectively. The fabricated all-solid-state device showed a high specific capacitance of 244 F/g with an energy density of 8.6 Wh/kg. In an ionic liquid electrolyte, the fabricated device showed a high specific capacitance of 126 F/g and a wide potential window up to 3 V, which results in a high energy density of 40 Wh/kg. Furthermore, it was observed that the activation temperature has significant role in the electrochemical performance, as the activated sample at 700 °C showed best activity than the samples activated at 600 and 800 °C. The electron microscopic images (FE-SEM and HR-TEM) confirmed the formation of pores by the chemical activation. A fabricated supercapacitor device in ionic liquid with 3 V could power up a red LED for 30 min upon charging for 20s. Also, it is shown that the operation voltage and capacitance of flexible all-solid-state symmetric supercapacitors fabricated using aloe-derived activated carbon could be easily tuned by series and parallel combinations. The performance of fabricated supercapacitor devices using A. vera derived activated carbon in all-solid-state and ionic liquid indicates their viable applications in flexible devices and energy storage.

Virulence factors analysis of Staphylococcus aureus isolated from bovine mastitis in México

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Julio C. Franco G.

2008-01-01

Full Text Available Un total de 117 aislados bacterianos obtenidos de casos de mastitis bovina positivos a las pruebas de catalasa, coagulasa, termonucleasa, manitol y PCR fueron analizados para determinar la producción de los siguientes factores de virulencia: hemolisisnas (alfa y beta, factor de agregación y cápsula. Adicionalmente fue también evaluada la sensibilidad a oxitetraciclina, penicilina, oxacilina y estreptomicina. La producción de hemolisinas y el factor de agregación fueron los 2 factores de virulencia expresados en la mayoría de los aislados (78% y (83% respectivamente, mientras que la formación de cápsula se detectó en 31% de los aislados bovinos. Una fuerte correlación entre ausencia de cápsula y producción de hemolisinas fue observado, ya que 72 de un total de 92 cepas hemolíticas no mostraron cápsula. La ausencia de cápsula fue asociada a la producción del factor de agregación en 89% de los aislados bacterianos positivos a este factor. El 68% y 69% de los aislados bacterianos resultaron positivos a penicilina y oxacilina respectivamente. La mayoría de los aislados (116 expresaron al menos 1 factor de virulencia (hemolisinas, cápsula o factor de agregación. Finalmente, el papel de los factores de virulencia y su relación en la patogenicidad de S. aureus en la mastitis bovina en México es discutido.

La precocidad en la autobiografía mexicana: un proyecto editorial. Nuevos escritores mexicanos del siglo XX presentados por sí mismos