Sample records for adjacent mismatch base

  1. Heterodyne detection with mismatch correction base on array detector

    Hongzhou, Dong; Guoqiang, Li; Ruofu, Yang; Chunping, Yang; Mingwu, Ao


    Based on an array detector, a new heterodyne detection system, which can correct the mismatches of amplitude and phase between signal and local oscillation (LO) beams, is presented in this paper. In the light of the fact that, for a heterodyne signal, there is a certain phase difference between the adjacent two samples of analog-to-digital converter (ADC), we propose to correct the spatial phase mismatch by use of the time-domain phase difference. The corrections can be realized by shifting the output sequences acquired from the detector elements in the array, and the steps of the shifting depend on the quantity of spatial phase mismatch. Numerical calculations of heterodyne efficiency are conducted to confirm the excellent performance of our system. Being different from previous works, our system needs not extra optical devices, so it provides probably an effective means to ease the problem resulted from the mismatches.

  2. Heterodyne detection with mismatch correction based on array detector

    Dong, Hongzhou; Li, Guoqiang; Yang, Ruofu; Yang, Chunping; Ao, Mingwu


    Based on an array detector, a new heterodyne detection system, which can correct the mismatches of amplitude and phase between signal and local oscillation (LO) beams, is presented in this paper. In the light of the fact that, for a heterodyne signal, there is a certain phase difference between the adjacent two samples of analog-to-digital converter (ADC), we propose to correct the spatial phase mismatch by use of the time-domain phase difference. The corrections can be realized by shifting the output sequences acquired from the detector elements in the array, and the steps of the shifting depend on the quantity of spatial phase mismatch. Numerical calculations of heterodyne efficiency are conducted to confirm the excellent performance of our system. Being different from previous works, our system needs not extra optical devices, so it provides probably an effective means to ease the problem resulted from the mismatches.

  3. The effect of base pair mismatch on DNA strand displacement

    Broadwater, Bo


    DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized. Here, we measured toehold-mediated strand displacement kinetics using single-molecule fluorescence in the presence of a single base pair mismatch. The apparent displacement rate varied significantly when the mismatch was introduced in the invading DNA strand. The rate generally decreased as the mismatch in the invader was encountered earlier in displacement. Our data indicate that a single base pair mismatch in the invader stalls branch migration, and displacement occurs via direct dissociation of the destabilized incumbent strand from the substrate strand. We combined both branch migration and direct dissociation into a model, which we term, the concurrent displacement model, and used the first passage t...

  4. Single-base mismatch detection based on charge transduction through DNA.

    Kelley, S O; Boon, E M; Barton, J K; Jackson, N M; Hill, M. G.


    High-throughput DNA sensors capable of detecting single-base mismatches are required for the routine screening of genetic mutations and disease. A new strategy for the electrochemical detection of single-base mismatches in DNA has been developed based upon charge transport through DNA films. Double-helical DNA films on gold surfaces have been prepared and used to detect DNA mismatches electrochemically. The signals obtained from redox-active intercalators bound to DNA-modified gold surfaces d...

  5. Reactivity of potassium permanganate and tetraethylammonium chloride with mismatched bases and a simple mutation detection protocol.

    Lambrinakos, A; Humphrey, K E; Babon, J J; Ellis, T P; Cotton, R G


    Many mutation detection techniques rely upon recognition of mismatched base pairs in DNA hetero-duplexes. Potassium permanganate in combination with tetraethylammonium chloride (TEAC) is capable of chemically modifying mismatched thymidine residues. The DNA strand can then be cleaved at that point by treatment with piperidine. The reactivity of potassium permanganate (KMnO4) in TEAC toward mismatches was investigated in 29 different mutations, representing 58 mismatched base pairs and 116 mis...

  6. Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA

    Cristovao, M.; Sisamakis, E.; Hingorani, M M; Marx, A. D.; Jung, C. P.; Rothwell, P. J.; Seidel, C. A. M.; Friedhoff, P.


    Mismatch repair (MMR) corrects replication errors such as mismatched bases and loops in DNA. The evolutionarily conserved dimeric MMR protein MutS recognizes mismatches by stacking a phenylalanine of one subunit against one base of the mismatched pair. In all crystal structures of G:T mismatch-bound MutS, phenylalanine is stacked against thymine. To explore whether these structures reflect directional mismatch recognition by MutS, we monitored the orientation of Escherichia coli MutS binding ...

  7. Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA

    M. Cristovao (Michele); E. Sisamakis (Evangelos); M.M. Hingorani (Manju); A.D. Marx (Andreas); C.P. Jung (Caroline); P.J. Rothwell (Paul); C.A.M. Seidel (Claus A.); P. Friedhoff (Peter)


    textabstractMismatch repair (MMR) corrects replication errors such as mismatched bases and loops in DNA. The evolutionarily conserved dimeric MMR protein MutS recognizes mismatches by stacking a phenylalanine of one subunit against one base of the mismatched pair. In all crystal structures of G:T mi

  8. Model Mismatch Paradigm for Probe based Nanoscale Imaging

    Agarwal, Pranav

    Scanning Probe Microscopes (SPMs) are widely used for investigation of material properties and manipulation of matter at the nanoscale. These instruments are considered critical enablers of nanotechnology by providing the only technique for direct observation of dynamics at the nanoscale and affecting it with sub Angstrom resolution. Current SPMs are limited by low throughput and lack of quantitative measurements of material properties. Various applications like the high density data storage, sub-20 nm lithography, fault detection and functional probing of semiconductor circuits, direct observation of dynamical processes involved in biological samples viz. motor proteins and transport phenomena in various materials demand high throughput operation. Researchers involved in material characterization at nanoscale are interested in getting quantitative measurements of stiffness and dissipative properties of various materials in a least invasive manner. In this thesis, system theoretic concepts are used to address these limitations. The central tenet of the thesis is to model, the known information about the system and then focus on perturbations of these known dynamics or model, to sense the effects due to changes in the environment such as changes in material properties or surface topography. Thus a model mismatch paradigm for probe based nanoscale imaging is developed. The topic is developed by presenting physics based modeling of a particular mode of operation of SPMs called the dynamic mode operation. This mode is modeled as a forced Lure system where a linear time invariant system is in feedback with an unknown static memoryless nonlinearity. Tools from averaging theory are used to tame this complex nonlinear system by approximating it as a linear system with time varying parameters. Material properties are thus transformed from being parameters of unknown nonlinear functions to being unknown coefficients of a linear plant. The first contribution of this thesis

  9. A polymerization-based method to construct a plasmid containing clustered DNA damage and a mismatch.

    Takahashi, Momoko; Akamatsu, Ken; Shikazono, Naoya


    Exposure of biological materials to ionizing radiation often induces clustered DNA damage. The mutagenicity of clustered DNA damage can be analyzed with plasmids carrying a clustered DNA damage site, in which the strand bias of a replicating plasmid (i.e., the degree to which each of the two strands of the plasmid are used as the template for replication of the plasmid) can help to clarify how clustered DNA damage enhances the mutagenic potential of comprising lesions. Placement of a mismatch near a clustered DNA damage site can help to determine the strand bias, but present plasmid-based methods do not allow insertion of a mismatch at a given site in the plasmid. Here, we describe a polymerization-based method for constructing a plasmid containing clustered DNA lesions and a mismatch. The presence of a DNA lesion and a mismatch in the plasmid was verified by enzymatic treatment and by determining the relative abundance of the progeny plasmids derived from each of the two strands of the plasmid. PMID:27449134


    Monica Mihaela MAER MATEI


    Full Text Available The research developed in this paper is based on micro data available in Programme for the International Assessment of Adult Competencies (PIAAC. The research aimed to estimate the size of both forms of labour market mismatch: education mismatch and skill mismatch. The first measure of job mismatch is based on workers’ self-assessment. The second one uses the PIAAC assessment regarding the proficiency for each skill dimension (literacy, numeracy and problem solving in technology rich environments. The labor market mismatch was measured for Spain and Italy datasets for the higher education graduates whose occupations are included in Major Group two Professionals, according to the International Standard Classification of Occupations. The estimation results showed that the two measures of labour market mismatch are not correlated.

  11. Performance Analysis of Spectral Amplitude Coding Based OCDMA System with Gain and Splitter Mismatch

    Umrani, Fahim A.; Umrani, A. Waheed; Umrani, Naveed A.; Memon, Kehkashan A.; Kalwar, Imtiaz Hussain


    This paper presents the practical analysis of the optical code-division multiple-access (O-CDMA) systems based on perfect difference codes. The work carried out use SNR criterion to select the optimal value of avalanche photodiodes (APD) gain and shows how the mismatch in the splitters and gains of the APD used in the transmitters and receivers of network can degrade the BER performance of the system. The investigations also reveal that higher APD gains are not suitable for such systems even at higher powers. The system performance, with consideration of shot noise, thermal noise, bulk and surface leakage currents is also investigated.

  12. Effect of base mismatch on the electronic properties of DNA-DNA and LNA-DNA double strands: Density-functional theoretical calculations

    Natsume, Takayuki; Ishikawa, Yasuyuki; Dedachi, Kenichi; Tsukamoto, Takayuki; Kurita, Noriyuki


    The electronic properties of double-stranded octametric DNA-DNA and LNA-DNA with a single-base mismatch were compared with those having fully complementary base pairs to quantify the effect of the base mismatch on hybridization energies (HE). A single T-G mismatch in the LNA-DNA gives rise to a significant reduction in HE, which is consistent with a significant lowering of the melting temperature for mismatched LNA-DNA. By contrast, the hybridization strength of the mismatched DNA-DNA depends...

  13. Compensating Acoustic Mismatch Using Class-Based Histogram Equalization for Robust Speech Recognition

    Suh, Youngjoo; Kim, Sungtak; Kim, Hoirin


    A new class-based histogram equalization method is proposed for robust speech recognition. The proposed method aims at not only compensating for an acoustic mismatch between training and test environments but also reducing the two fundamental limitations of the conventional histogram equalization method, the discrepancy between the phonetic distributions of training and test speech data, and the nonmonotonic transformation caused by the acoustic mismatch. The algorithm employs multiple class-specific reference and test cumulative distribution functions, classifies noisy test features into their corresponding classes, and equalizes the features by using their corresponding class reference and test distributions. The minimum mean-square error log-spectral amplitude (MMSE-LSA)-based speech enhancement is added just prior to the baseline feature extraction to reduce the corruption by additive noise. The experiments on the Aurora2 database proved the effectiveness of the proposed method by reducing relative errors by[InlineEquation not available: see fulltext.] over the mel-cepstral-based features and by[InlineEquation not available: see fulltext.] over the conventional histogram equalization method, respectively.

  14. Blind Decorrelating Detection Based on Particle Swarm Optimization under Spreading Code Mismatch

    Jhih-Chung Chang; Chih-Chang Shen


    A way of resolving spreading code mismatches in blind multiuser detection with a particle swarm optimization (PSO) approach is proposed. It has been shown that the PSO algorithm incorporating the linear system of the decorrelating detector, which is termed as decorrelating PSO (DPSO), can significantly improve the bit error rate (BER) and the system capacity. As the code mismatch occurs, the output BER performance is vulnerable to degradation for DPSO. With a blind decorrelating scheme, the proposed blind DPSO (BDPSO) offers more robust capabilities over existing DPSO under code mismatch scenarios.

  15. Low frequency of defective mismatch repair in a population-based series of upper urothelial carcinoma

    Isfoss Björn L


    Full Text Available Abstract Background Upper urothelial cancer (UUC, i.e. transitional cell carcinomas of the renal pelvis and the ureter, occur at an increased frequency in patients with hereditary nonpolyposis colorectal cancer (HNPCC. Defective mismatch repair (MMR specifically characterizes HNPCC-associated tumors, but also occurs in subsets of some sporadic tumors, e.g. in gastrointestinal cancer and endometrial cancer. Methods We assessed the contribution of defective MMR to the development of UUC in a population-based series from the southern Swedish Cancer Registry, through microsatellite instability (MSI analysis and immunohistochemical evaluation of expression of the MMR proteins MLH1, PMS2, MSH2, and MSH6. Results A MSI-high phenotype was identified in 9/216 (4% successfully analyzed patients and a MSI-low phenotype in 5/216 (2%. Loss of MMR protein immunostaining was found in 11/216 (5% tumors, and affected most commonly MSH2 and MSH6. Conclusion This population-based series indicates that somatic MMR inactivation is a minor pathway in the development of UUC, but tumors that display defective MMR are, based on the immunohistochemical expression pattern, likely to be associated with HNPCC.

  16. Non-target adjacent stimuli classification improves performance of classical ERP-based brain computer interface

    Ceballos, G. A.; Hernández, L. F.


    Objective. The classical ERP-based speller, or P300 Speller, is one of the most commonly used paradigms in the field of Brain Computer Interfaces (BCI). Several alterations to the visual stimuli presentation system have been developed to avoid unfavorable effects elicited by adjacent stimuli. However, there has been little, if any, regard to useful information contained in responses to adjacent stimuli about spatial location of target symbols. This paper aims to demonstrate that combining the classification of non-target adjacent stimuli with standard classification (target versus non-target) significantly improves classical ERP-based speller efficiency. Approach. Four SWLDA classifiers were trained and combined with the standard classifier: the lower row, upper row, right column and left column classifiers. This new feature extraction procedure and the classification method were carried out on three open databases: the UAM P300 database (Universidad Autonoma Metropolitana, Mexico), BCI competition II (dataset IIb) and BCI competition III (dataset II). Main results. The inclusion of the classification of non-target adjacent stimuli improves target classification in the classical row/column paradigm. A gain in mean single trial classification of 9.6% and an overall improvement of 25% in simulated spelling speed was achieved. Significance. We have provided further evidence that the ERPs produced by adjacent stimuli present discriminable features, which could provide additional information about the spatial location of intended symbols. This work promotes the searching of information on the peripheral stimulation responses to improve the performance of emerging visual ERP-based spellers.

  17. A method of 3D CAD model retrieval based on feature adjacent graph

    Shi, Min; Zhang, Shusheng


    To reuse the 3D CAD model, a new method of 3D CAD model retrieval based on feature adjacent graph is proposed. First, a representation of feature adjacent graph for the CAD model is extracted by automatic identification-based and interactive identification-assisted. Next, the vertex product graph of the two CAD model's feature adjacent graphs is constructed, and then the ant colony algorithm is employed to detect the maximum-cliques in the vertex product graph. At last, the similarity of the models is calculated by comparing the maximum-cliques. Experimental results show that this method can realize the 3D CAD model retrieval and its efficiency meets the requirements of applications.

  18. The γ/γ ' mismatch in Ni based superalloys: In situ measurements during a creep test

    Diologent, F.; Caron, P.; d'Almeida, T.; Jacques, A.; Bastie, P.


    The lattice mismatch between the fcc γ matrix and the ordered γ ' cuboı̈dal precipitates in superalloys induces large internal stresses within the material. These stresses have a major effect on its mechanical behaviour and on the anisotropic evolution of the microstructure (rafting…) during its lifetime. The evolution of the effective lattice mismatch of the AM1 and MCNG superalloys was measured continuously during high temperature creep tests (1100 °C, 150 MPa) at the ID 15 (high energy) beamline of the ESRF. The bulk profiles of the 200 reflection (parallel to the tensile axis) were recorded using the triple crystal diffractometer. Both materials have a negative mismatch, and exhibit a transition between the initial wide γ ' peak to a two peaks profile during stage I of the creep curve, as rafting takes place. During stage II, the 200 mismatch decreases in magnitude. During stage III, as a microstructural transition (coalescence) occurs and the strain rate increases, the mismatch changes in the same direction, but at a faster rate, while the thickness of both peaks increases. Evolution of the MCNG specimen was the same as AM1 ones, but rafting and transition to stage II take place at a different rate.

  19. Label-free detection of DNA single-base mismatches using a simple reflectance-based optical technique.

    Nava, G; Ceccarello, E; Giavazzi, F; Salina, M; Damin, F; Chiari, M; Buscaglia, M; Bellini, T; Zanchetta, G


    Rapid and quantitative detection of the binding of nucleic acids to surface-immobilized probes remains a challenge in many biomedical applications. We investigated the hybridization of a set of fully complementary and defected 12-base long DNA oligomers by using the Reflective Phantom Interface (RPI), a recently developed multiplexed label-free detection technique. Based on the simple measurement of reflected light intensity, this technology enables to quantify the hybridization directly as it occurs on the surface with a sensitivity of 10 pg mm(-2). We found a strong effect of single-base mismatches and of their location on hybridization kinetics and equilibrium binding. In line with previous studies, we found that DNA-DNA binding is weaker on a surface than in the bulk. Our data indicate that this effect is a consequence of weak nonspecific binding of the probes to the surface. PMID:27122358

  20. Gold-based optical biosensor for single-mismatched DNA detection using salt-induced hybridization

    Zhan, Zongrui; Ma, Xingyi; Cao, Cuong;


    DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the...... presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which...

  1. Magnetostatic-Wave-Based Magneto-Optic Pulse Compression by Control of Phase Mismatching

    WU Bao-Jian; GAO Xiang


    Microwave magnetostatic waves (MSWs) as moving gratings in magneto-optic (MO) film can lead to the Bragg diffraction of guided optical waves (GOWs). The MO coupling characteristics are responsible for the amplitude and phase frequency spectra of diffracted pulses and even result in the compression of chirped optical pulses in time domain. We theoretically investigate the noncollinear diffraction of linearly chirped Gaussian optical pulses by continuous magnetostatic forward volume waves in detail. For a given chirped optical pulse, with the increase of phase-mismatching slopes, the compression efficiency (CE) is gradually improved up to the maximum followed by the transition of diffracted pulses from single peak to multi peaks. The larger the chirp parameter is,the smaller the required phase-mismatching slope to achieve the maximal CE is. However, the rise of the chirp parameter or phase-mismatching slope reduces the relative peak intensity of the diffracted pulse. Lastly, it is pointed out that the phase-mismatching slope can be greatly increased by using the high-order modes of MSWs and GOWs.

  2. Statistical weighting of model-based optoacoustic reconstruction for minimizing artefacts caused by strong acoustic mismatch

    Deán-Ben, X. Luís; Razansky, Daniel; Ntziachristos, Vasilis


    A modified quantitative inversion algorithm is presented that minimizes the effects of internal acoustic reflections or scattering in tomographic optoacoustic images. The inversion procedure in our model-based algorithm consists in solving a linear system of equations in which each individual equation corresponds to a given position of the acoustic transducer and to a given time instant. Thus, the modification that we propose in this work consists in weighting each equation of the linear system with the probability that the measured wave is not distorted by reflection or scattering phenomena. We show that the probability that a reflected or scattered wave is detected at a given position and at a given instant is approximately proportional to the size of the area in which the original wave could have been generated, which is dependent on the position of the transducer and on the time instant, so that such probability can be used to weight each equation of the linear system. Thereby, the contribution of the waves that propagate directly to the transducer to the reconstructed images is emphasized. We experimentally test the proposed inversion algorithm with tissue-mimicking agar phantoms in which air-gaps are included to cause reflections of the acoustic waves. The tomographic reconstructions obtained with the modification proposed herein show a clear reduction of the artefacts due to these acoustic phenomena with respect to the reconstructions yielded with the original algorithm. This performance is directly related to in-vivo small animal imaging applications involving imaging in the presence of bones, lungs, and other highly mismatched organs.

  3. The Escherichia coli Methyl-Directed Mismatch Repair System Repairs Base Pairs Containing Oxidative Lesions

    Wyrzykowski, Jennifer; Volkert, Michael R.


    A major role of the methyl-directed mismatch repair (MMR) system of Escherichia coli is to repair postreplicative errors. In this report, we provide evidence that MMR also acts on oxidized DNA, preventing mutagenesis. When cells deficient in MMR are grown anaerobically, spontaneous mutation frequencies are reduced compared with those of the same cells grown aerobically. In addition, we show that a dam mutant has an increased sensitivity to hydrogen peroxide treatment that can be suppressed by...

  4. Analysis of the effects of mismatched errors on coherent beam combining based on a self-imaging waveguide

    Tao, R.; Wang, X.; Zhou, Pu; Si, Lei


    A theoretical model of coherent beam combining (CBC) based on a self-imaging waveguide (SIW) is built and the effects of mismatched errors on SIW-based CBC are simulated and analysed numerically. With the combination of the theoretical model and the finite difference beam propagation method, two main categories of errors, assembly and nonassembly errors, are numerically studied to investigate their effect on the beam quality by using the M2 factor. The optimisation of the SIW and error control principle of the system is briefly discussed. The generalised methodology offers a good reference for investigating waveguide-based high-power coherent combining of fibre lasers in a comprehensive way.

  5. Estimation of channel mismatches in time-interleaved analog-to-digital converters based on fractional delay and sine curve fitting.

    Guo, Lianping; Tian, Shulin; Jiang, Jun


    This paper proposes an algorithm to estimate the channel mismatches in time-interleaved analog-to-digital converter (TIADC) based on fractional delay (FD) and sine curve fitting. Choose one channel as the reference channel and apply FD to the output samples of reference channel to obtain the ideal samples of non-reference channels with no mismatches. Based on least square method, the sine curves are adopted to fit the ideal and the actual samples of non-reference channels, and then the mismatch parameters can be estimated by comparing the ideal sine curves and the actual ones. The principle of this algorithm is simple and easily understood. Moreover, its implementation needs no extra circuits, lowering the hardware cost. Simulation results show that the estimation accuracy of this algorithm can be controlled within 2%. Finally, the practicability of this algorithm is verified by the measurement results of channel mismatch errors of a two-channel TIADC prototype. PMID:25832264

  6. Strategy in clinical practice for classification of unselected colorectal tumours based on mismatch repair deficiency

    Jensen, Lars Henrik; Lindebjerg, J; Byriel, L;


    Objective Deficiency of DNA mismatch repair (MMR) causes microsatellite instability (MSI) in a subset of colorectal cancers. Patients with these tumours have a better prognosis and may have an altered response to chemotherapy. Some of the tumours are caused by hereditary mutations (hereditary non...... methylation or BRAF mutation analysis to distinguish sporadic patients from likely hereditary ones. MMR deficient patients with sporadic disease can be reassured of the better prognosis and the likely hereditary cases should receive genetic counselling.......Objective Deficiency of DNA mismatch repair (MMR) causes microsatellite instability (MSI) in a subset of colorectal cancers. Patients with these tumours have a better prognosis and may have an altered response to chemotherapy. Some of the tumours are caused by hereditary mutations (hereditary...... nonpolyposis colon cancer or Lynch syndrome), but most are epigenetic changes of sporadic origin. The aim of this study was to define a robust and inexpensive strategy for such classification in clinical practice. Method Tumours and blood samples from 262 successive patients with colorectal adenocarcinomas...

  7. A Layered Zone Routing Algorithm in Ad Hoc Network Based on Matrix of Adjacency Connection

    XU Guang-wei; LI Feng; SHI Xiu-jin; HUO Jia-zhen


    The hybrid routing protocol has received more attention recently than the proactive and the reactive, especially for large-scale and highly dynamic connection, in mobile ad hoc network. A crucial reason is that zone-layered is being utilized in the complex systems. A hybrid routing algorithm which is layered zone based on adjacency connection(LZBAC) is put forward under the background of a few members in network with steady position and link. The algorithm modifies storage structure of nodes and improves routing mechanism. The theoretical analysis and simulation testing testify that the algorithm costs shorter time of route finding and less delay than others.

  8. Adjacency Matrix Based Energy Efficient Scheduling Using S-Mac Protocol in Wireless Sensor Networks

    Ravindara Bhatt,


    Full Text Available Communication is the main motive in any Networks whether it is Wireless Sensor Network, Ad-Hoc networks, Mobile Networks, Wired Networks, Local Area Network, Metropolitan Area Network, Wireless Area Network etc, hence it must be energy efficient. The main parameters for energy efficient communication are maximizing network lifetime, saving energy at the different nodes, sending the packets in minimum time delay, higher throughput etc. This paper focuses mainly on the energy efficient communication with the help of Adjacency Matrix in the Wireless Sensor Networks. The energy efficient scheduling can be done by putting the idle node in to sleep node so energy at the idle node can be saved. The proposed model in this paper first forms the adjacency matrix and broadcasts the information aboutthe total number of existing nodes with depths to the other nodes in the same cluster from controller node. When every node receives the node information about the other nodes for same cluster they communicate based on the shortest depths and schedules the idle node in to sleep mode for a specific time threshold so energy at the idle nodes can be saved.

  9. Effect of LNA- and OMeN-modified oligonucleotide probes on the stability and discrimination of mismatched base pairs of duplexes

    Ying Yan; Jing Yan; Xianyu Piao; Tianbiao Zhang; Yifu Guan


    Locked nucleic acid (LNA) and 2′--methyl nucleotide (OMeN) are the most extensively studied nucleotide analogues. Although both LNA and OMeN are characterized by the C3′-endo sugar pucker conformation, which is dominant in A-form DNA and RNA nucleotides, they demonstrate different binding behaviours. Previous studies have focused attention on their properties of duplex stabilities, hybridization kinetics and resistance against nuclease digestion; however, their ability to discriminate mismatched hybridizations has been explored much less. In this study, LNA- and OMeN-modified oligonucleotide probes have been prepared and their effects on the DNA duplex stability have been examined: LNA modifications can enhance the duplex stability, whereas OMeN modifications reduce the duplex stability. Next, we studied how the LNA:DNA and OMeN:DNA mismatches reduced the duplex stability. Melting temperature measurement showed that different LNA:DNA or OMeN:DNA mismatches indeed influence the duplex stability differently. LNA purines can discriminate LNA:DNA mismatches more effectively than LNA pyrimidines as well as DNA nucleotides. Furthermore, we designed five LNA- and five OMeN-modified oligonucleotide probes to simulate realistic situations where target–probe duplexes contain a complementary LNA:DNA or OMeN:DNA base pairs and a DNA:DNA mismatch simultaneously. The measured collective effect showed that the duplex stability was enhanced by the complementary LNA:DNA base pair but decreased by the DNA:DNA mismatch in a position-dependent manner regardless of the chemical identity and position of the complementary LNA:DNA base pair. On the other hand, the OMeN-modified probes also showed that the duplex stability was reduced by both the OMeN modification and the OMeN:DNA mismatch in a position-dependent manner.

  10. A modified Kelvin impact model for pounding simulation of base-isolated building with adjacent structures

    Ye Kun; Li Li; Zhu Hongping


    Base isolation can effectively reduce the seismic forces on a superstructure, particularly in low- to medium-rise buildings. However, under strong near-fault ground motions, pounding may occur at the isolation level between the base-isolated building (BIB) and its surrounding retaining walls. To effectively investigate the behavior of the BIB pounding with adjacent structures, after assessing some commonly used impact models, a modified Kelvin impact model is proposed in this paper. Relevant parameters in the modified Kelvin model are theoretically derived and numerically verified through a simple pounding case. At the same time, inelasticity of the isolated superstructure is introduced in order to accurately evaluate the potential damage to the superstructure caused by the pounding of the BIB with adjacent structures. The reliability of the modified Kelvin impact model is validated through numerical comparisons with other impact models. However, the difference between the numerical results from the various impact analytical models is not significant. Many numerical simulations of BIBs are conducted to investigate the influence of various design parameters and conditions on the peak inter-story drills and floor accelerations during pounding. It is shown that pounding can substantially increase floor accelerations, especially at the ground floor where impacts occur. Higher modes of vibration are excited during poundings, increasing the inter-story drifts instead of keeping a nearly rigid-body motion of the superstructure. Furthermore, higher ductility demands can be imposed on lower floors of the superstructure. Moreover, impact stiffness seems to play a significant role in the acceleration response at the isolation level and the inter-story drifts of lower floors of the superstructure. Finally, the numerical results show that excessive flexibility of the isolation system used to minimize the floor accelerations may cause the BIB to be more susceptible to pounding

  11. Medical image classification using spatial adjacent histogram based on adaptive local binary patterns.

    Liu, Dong; Wang, Shengsheng; Huang, Dezhi; Deng, Gang; Zeng, Fantao; Chen, Huiling


    Medical image recognition is an important task in both computer vision and computational biology. In the field of medical image classification, representing an image based on local binary patterns (LBP) descriptor has become popular. However, most existing LBP-based methods encode the binary patterns in a fixed neighborhood radius and ignore the spatial relationships among local patterns. The ignoring of the spatial relationships in the LBP will cause a poor performance in the process of capturing discriminative features for complex samples, such as medical images obtained by microscope. To address this problem, in this paper we propose a novel method to improve local binary patterns by assigning an adaptive neighborhood radius for each pixel. Based on these adaptive local binary patterns, we further propose a spatial adjacent histogram strategy to encode the micro-structures for image representation. An extensive set of evaluations are performed on four medical datasets which show that the proposed method significantly improves standard LBP and compares favorably with several other prevailing approaches. PMID:27058283

  12. The {gamma}/{gamma}{sup '} mismatch in Ni based superalloys: In situ measurements during a creep test

    Diologent, F.; Caron, P.; D' Almeida, T.; Jacques, A. E-mail:; Bastie, P


    The lattice mismatch between the fcc {gamma} matrix and the ordered {gamma}{sup '} cuboiedal precipitates in superalloys induces large internal stresses within the material. These stresses have a major effect on its mechanical behaviour and on the anisotropic evolution of the microstructure (rafting...) during its lifetime. The evolution of the effective lattice mismatch of the AM1 and MCNG superalloys was measured continuously during high temperature creep tests (1100 deg. C, 150 MPa) at the ID 15 (high energy) beamline of the ESRF. The bulk profiles of the 200 reflection (parallel to the tensile axis) were recorded using the triple crystal diffractometer. Both materials have a negative mismatch, and exhibit a transition between the initial wide {gamma}{sup '} peak to a two peaks profile during stage I of the creep curve, as rafting takes place. During stage II, the 200 mismatch decreases in magnitude. During stage III, as a microstructural transition (coalescence) occurs and the strain rate increases, the mismatch changes in the same direction, but at a faster rate, while the thickness of both peaks increases. Evolution of the MCNG specimen was the same as AM1 ones, but rafting and transition to stage II take place at a different rate.

  13. Technical aspects of typing for HLA-DP alleles using allele-specific DNA in vitro amplification and sequence-specific oligonucleotide probes. Detection of single base mismatches

    Fugger, L; Morling, N; Ryder, L P;


    The polymerase chain reaction (PCR) is an effective method for in vitro DNA amplification which combined with probing with synthetic oligonucleotides can be used for, e.g., HLA-typing. We have studied the technical aspects of HLA-DP typing with the technique. DNA from mononuclear nucleated cells...... base mismatches may be detected in the PCR and typing for HLA-DP gene variants, which differ for only one base, may be performed....

  14. Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway

    Grippon, Seden; Zhao, Qiyuan; Robinson, Tom; Marshall, Jacqueline J. T.; O’Neill, Rory J.; Manning, Hugh; Kennedy, Gordon; Dunsby, Christopher; Neil, Mark; Halford, Stephen E.; French, Paul M. W.; Baldwin, Geoff S.


    Mismatch uracil DNA glycosylase (Mug) from Escherichia coli is an initiating enzyme in the base-excision repair pathway. As with other DNA glycosylases, the abasic product is potentially more harmful than the initial lesion. Since Mug is known to bind its product tightly, inhibiting enzyme turnover, understanding how Mug binds DNA is of significance when considering how Mug interacts with downstream enzymes in the base-excision repair pathway. We have demonstrated differential binding modes o...

  15. Method based on chirp decomposition for dispersion mismatch compensation in precision absolute distance measurement using swept-wavelength interferometry.

    Lu, Cheng; Liu, Guodong; Liu, Bingguo; Chen, Fengdong; Hu, Tao; Zhuang, Zhitao; Xu, Xinke; Gan, Yu


    We establish a theoretical model of dispersion mismatch in absolute distance measurements using swept-wavelength interferometry (SWI) and propose a novel dispersion mismatch compensation method called chirp decomposition. This method separates the dispersion coefficient and distance under test, which ensures dispersion mismatch compensation without introducing additional random errors. In the measurement of a target located at 3.9 m, a measurement resolution of 45.9 μm is obtained, which is close to the theoretical resolution, and a standard deviation of 0.74 μm is obtained, which is better than the traditional method. The measurement results are compared to a single-frequency laser interferometer. The target moves from 1 m to 3.7 m, and the measurement precision using the new method is less than 0.81 μm. PMID:26698959

  16. NMR study of oligonucleotides containing base pair mismatches and a human growth hormone peptide for the determination of solution structures

    Formation of unusual basepairs in DNA for random mutations in DNA was proposed in the sixties. These mismatches arise due to errors in replication, and from deamination of the 5-methylcytosine. The author's interest in studying mismatches and other oligonucleotides has been two fold. One is related to 31P chemical shifts and the backbone structure of oligonucleotides. He wanted to find out the significance of the dispersion of 31p chemical shifts in oligonucleotides. He wished to address whether this dispersion in 31P chemical shifts is related to global structural parameters of oligonucleotides like helix twist and whether he can prove the relationship between 31P chemical shifts and the backbone torsional angles epsilon and zeta. How does a mismatch affect 31P chemical shifts and the backbone torsional angle? The second interest is related to solving the three dimensional structure of these biopolymers by using NMR data (NOESY distances) and computer simulations. His major study of these mismatches has been in the assignments of the protons resonances and the phosphorus resonances by 2D NMR. He has also tried to answer the question about the relationships between 31P chemical shifts and global parameters for DNA such as the helix twist. He has made substantial progress in determination of J(H3'-P) coupling constants by 2D NMR and also in determining the relationship between the SIP chemical shifts and the backbone torsional angles by using the mismatch dodecamer sequences and the tetradecamer sequences. The 2D NMR data for the GG and GT mismatch have been used to determine three dimensional structures by using distance restrained molecular dynamics. The second project involved studying a 28 residue synthetic peptide by NMR

  17. Diagnosis of Constitutional Mismatch Repair-Deficiency Syndrome Based on Microsatellite Instability and Lymphocyte Tolerance to Methylating Agents

    Bodo, Sahra; Colas, Chrystelle; Buhard, Olivier;


    BACKGROUND & AIMS: Patients with bi-allelic germline mutations in mismatch repair (MMR) genes (MLH1, MSH2, MSH6, or PMS2) develop a rare but severe variant of Lynch syndrome called constitutional MMR deficiency (CMMRD). This syndrome is characterized by early-onset colorectal cancers, lymphomas o...

  18. Adaptive IF selection and IQ mismatch compensation in a low-IF GSM receiver

    This paper presents an algorithm that can adaptively select the intermediate frequency (IF) and compensate the IQ mismatch according to the power ratio of the adjacent channel interference to the desired signal in a low-IF GSM receiver. The IF can be adaptively selected between 100 and 130 kHz. Test result shows an improvement of phase error from 6.78° to 3.23°. Also a least mean squares (LMS) based IQ mismatch compensation algorithm is applied to improve image rejection ratio (IRR) for the desired signal along with strong adjacent channel interference. The IRR is improved from 29.1 to 44.3 dB in measurement. The design is verified in a low-IF GSM receiver fabricated in SMIC 0.13 μm RF CMOS process with a working voltage of 1.2 V. (semiconductor integrated circuits)

  19. Native and non-native speech sound processing and the neural mismatch responses: A longitudinal study on classroom-based foreign language learning.

    Jost, Lea B; Eberhard-Moscicka, Aleksandra K; Pleisch, Georgette; Heusser, Veronica; Brandeis, Daniel; Zevin, Jason D; Maurer, Urs


    Learning a foreign language in a natural immersion context with high exposure to the new language has been shown to change the way speech sounds of that language are processed at the neural level. It remains unclear, however, to what extent this is also the case for classroom-based foreign language learning, particularly in children. To this end, we presented a mismatch negativity (MMN) experiment during EEG recordings as part of a longitudinal developmental study: 38 monolingual (Swiss-) German speaking children (7.5 years) were tested shortly before they started to learn English at school and followed up one year later. Moreover, 22 (Swiss-) German adults were recorded. Instead of the originally found positive mismatch response in children, an MMN emerged when applying a high-pass filter of 3 Hz. The overlap of a slow-wave positivity with the MMN indicates that two concurrent mismatch processes were elicited in children. The children's MMN in response to the non-native speech contrast was smaller compared to the native speech contrast irrespective of foreign language learning, suggesting that no additional neural resources were committed to processing the foreign language speech sound after one year of classroom-based learning. PMID:25934634

  20. Fixed-base comb with window-non-adjacent form (NAF) method for scalar multiplication.

    Seo, Hwajeong; Kim, Hyunjin; Park, Taehwan; Lee, Yeoncheol; Liu, Zhe; Kim, Howon


    Elliptic curve cryptography (ECC) is one of the most promising public-key techniques in terms of short key size and various crypto protocols. For this reason, many studies on the implementation of ECC on resource-constrained devices within a practical execution time have been conducted. To this end, we must focus on scalar multiplication, which is the most expensive operation in ECC. A number of studies have proposed pre-computation and advanced scalar multiplication using a non-adjacent form (NAF) representation, and more sophisticated approaches have employed a width-w NAF representation and a modified pre-computation table. In this paper, we propose a new pre-computation method in which zero occurrences are much more frequent than in previous methods. This method can be applied to ordinary group scalar multiplication, but it requires large pre-computation table, so we combined the previous method with ours for practical purposes. This novel structure establishes a new feature that adjusts speed performance and table size finely, so we can customize the pre-computation table for our own purposes. Finally, we can establish a customized look-up table for embedded microprocessors. PMID:23881143

  1. Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium salts.

    Gogos, J.A.; Karayiorgou, M; Aburatani, H; Kafatos, F C


    In the presence of tetramethylammonium chloride, potassium permanganate specifically modifies mismatched thymines. Similarly, the modification of mismatched cytosines by hydroxylamine was enhanced by tetraethylammonium chloride. Modification followed by piperidine cleavage permits specific identification of the T and C mismatches and by extension, when the opposite DNA strand is analyzed, of A and G mismatches as well. These reactions can be performed conveniently with DNA immobilized on Hybo...

  2. Zero energy buildings and mismatch compensation factors

    Lund, Henrik; Marszal, Anna Joanna; Heiselberg, Per


    individual buildings and an element of economy of scale. For these three reasons mismatches should be dealt with at the aggregated level and not at the individual level of each building. Instead, this paper suggests to compensate the mismatch of a building by increasing (or decreasing) the capacity of the...... energy production unit. Based on historical data for the electricity supply area in western Denmark, this paper makes a first attempt to quantify mismatch compensation factors. The results indicate that such compensation factors are a little below one for buildings with photovoltaics (PV) and a little...

  3. A Mismatch EndoNuclease Array-Based Methodology (MENA for Identifying Known SNPs or Novel Point Mutations

    Josep M. Comeron


    Full Text Available Accurate and rapid identification or confirmation of single nucleotide polymorphisms (SNPs, point mutations and other human genomic variation facilitates understanding the genetic basis of disease. We have developed a new methodology (called MENA (Mismatch EndoNuclease Array pairing DNA mismatch endonuclease enzymology with tiling microarray hybridization in order to genotype both known point mutations (such as SNPs as well as identify previously undiscovered point mutations and small indels. We show that our assay can rapidly genotype known SNPs in a human genomic DNA sample with 99% accuracy, in addition to identifying novel point mutations and small indels with a false discovery rate as low as 10%. Our technology provides a platform for a variety of applications, including: (1 genotyping known SNPs as well as confirming newly discovered SNPs from whole genome sequencing analyses; (2 identifying novel point mutations and indels in any genomic region from any organism for which genome sequence information is available; and (3 screening panels of genes associated with particular diseases and disorders in patient samples to identify causative mutations. As a proof of principle for using MENA to discover novel mutations, we report identification of a novel allele of the beethoven (btv gene in Drosophila, which encodes a ciliary cytoplasmic dynein motor protein important for auditory mechanosensation.

  4. Research of China’s Credit Currency Mismatch

    Rui Cui; Weiyi Wang


    Large positive current mismatch exists in China at present, and with the continual development of financial reform, the risk of the current mismatch will gradually diffuse from the micro layer to the macro layer, so it is very important to study China’s credit currency mismatch. Based on the theoretical research of the credit currency mismatch, the formula of AECM is used to measure the level of China’s credit currency mismatch, and the causes are analyzed, and corresponding policy advices ar...

  5. Influence of mis-match of weld and base material creep properties on elevated temperature design of pressure vessels and piping

    The stress and strain concentrations developed at the weldments during the long time operation of pressure vessels and piping at high temperature due to the mis-match in the creep properties of weldment constituents (weld, heat affected zone and base metal) are estimated using detailed finite element analysis. Three materials, viz. 2.25Cr 1Mo, SS 316 LN and modified 9Cr 1 Mo which are the most commonly used materials in the nuclear and thermal power plants are considered. A longitudinal seam weld with single and double V (X) configurations are analysed. Parametric studies have been done on weld angle and stresses. Based on the analysis, critical locations and the maximum stress concentration factors in the weldments for the above materials are identified. The weld design procedures of the currently used pressure vessel and piping codes are commented. The importance of ductility based failure criteria is emphasised. (orig.)

  6. $p$-th Clustering coefficients $C_{p}$ and Adjacent Matrix for Networks: Formulation based on String

    Toyota, Norihito


    The phenomenon of six degrees of separation is an old but interesting problem. The considerations of the clustering coefficient reflecting triangular structures and its extension to square one to six degrees of separation have been made\\cite{Newm21}. Recently, Aoyama\\cite{Aoyama} has given some considerations to this problem in networks without loops, using a sort of general formalism, "string formalism". In this article, we describe relations between the string formulation proposed by Aoyama and an adjacent matrix. Thus we provided a reformulation of the string formulation proposed by \\cite{Aoyama} to analyze networks. According to it, we introduced a series of generalized $q$-$th$ clustering coefficients. The available rules between diagrams of graphs and formulae are also given based on the formulation. Next we apply the formulation to some subjects in order to mainly check consistency with former studies. By evaluating the clustering coefficient for typical networks studied well earlier, we confirm a vali...

  7. MutS recognition: Multiple mismatches and sequence context effects

    Amita Joshi; Basuthkar J Rao


    Escherichia coli MutS is a versatile repair protein that specifically recognizes not only various types of mismatches but also single stranded loops of up to 4 nucleotides in length. Specific binding, followed by the next step of tracking the DNA helix that locates hemi-methylated sites, is regulated by the conformational state of the protein as a function of ATP binding/hydrolysis. Here, we study how various molecular determinants of a heteroduplex regulate mismatch recognition by MutS, the critical first step of mismatch repair. Using classical DNase I footprinting assays, we demonstrate that the hierarchy of MutS binding to various types of mismatches is identical whether the mismatches are present singly or in multiples. Moreover, this unique hierarchy is indifferent both to the differential level of DNA helical flexibility and to the unpaired status of the mismatched bases in a heteroduplex. Surprisingly, multiple mismatches exhibit reduced affinity of binding to MutS, compared to that of a similar single mismatch. Such a reduction in the affinity might be due to sequence context effects, which we established more directly by studying two identical single mismatches in an altered sequence background. A mismatch, upon simply being flipped at the same location, elicits changes in MutS specific contacts, thereby underscoring the importance of sequence context in modulating MutS binding to mismatches.

  8. Strategic Mismatches in Competing Teams

    Kräkel, Matthias


    This paper discusses the strategic role of mismatching, where players voluntarily form inefficient teams or forego the formation of efficient teams, respectively. Strategic mismatching can be rational when players realize a competitive advantage (e.g. harming other competitors). In addition, the results show that free riding can be beneficial for a team in combination with strategic mismatching and that the loser?s curse may be welfare improving by mitigating the problem of strategic mismatch...

  9. Human DNA mismatch repair: coupling of mismatch recognition to strand-specific excision

    WANG Huixian; Hays, John B.


    Eukaryotic mismatch-repair (MMR) proteins MutSα and MutLα couple recognition of base mismatches to strand-specific excision, initiated in vivo at growing 3′ ends and 5′ Okazaki-fragment ends or, in human nuclear extracts, at nicks in exogenous circular substrates. We addressed five biochemical questions relevant to coupling models. Excision remained fully efficient at DNA:MutSα ratios of nearly 1 to 1 at various mismatch-nick distances, suggesting a requirement for only one MutSα molecule per...

  10. Anomalous cross-linking by mechlorethamine of DNA duplexes containing C-C mismatch pairs.

    Romero, R M; Mitas, M; Haworth, I S


    Nitrogen mustards such as mechlorethamine have previously been shown to covalently cross-link DNA through the N7 position of the two guanine bases of a d[GXC].d[GYC] duplex sequence, a so-called 1,3 G-G-cross-link, when X-Y = C-G or T-A. Here, we report the formation of a new mechlorethamine cross-link with the d[GXC].d[GYC] fragment when X-Y is a C-C mismatch pair. Mechlorethamine cross-links this fragment preferentially between the two mismatched cytosine bases, rather than between the guanine bases. The cross-link also forms when one or both of the guanine bases of the d[GCC].d[GCC] fragment are replaced by N7-deazaguanine, and, more generally, forms with any C-C mismatch, regardless of the flanking base pairs. Piperidine cleavage of the cross-link species containing the d[GCC].d[GCC] sequence gives DNA fragments consistent with alkylation at the mismatched cytosine bases. We also provide evidence that the cross-link reaction occurs between the N3 atoms of the two cytosine bases by showing that the formation of the C-C cross-link is pH dependent for both mechlorethamine and chlorambucil. Dimethyl sulfate (DMS) probing of the cross-linked d[GCC].d[GCC] fragment showed that the major groove of the guanine adjacent to the C-C mismatch is still accessible to DMS. In contrast, the known minor groove binder Hoechst 33258 inhibits the cross-link formation with a C-C mismatch pair flanked by A-T base pairs. These results suggest that the C-C mismatch is cross-linked by mechlorethamine in the minor groove. Since C-C pairs may be involved in unusual secondary structures formed by the trinucleotide repeat sequence d[CCG]n, and associated with triplet repeat expansion diseases, mechlorethamine may serve as a useful probe for these structures. PMID:10090751

  11. An analytical study of mismatched complementary media

    Zhu, Lin; Ma, Hongru


    Complementary media (CM) interacting with arbitrarily situated obstacles are usually less discussed. In this paper, an analytical framework based on multiple scattering theory is established for analyzing such a mismatched case. As examples, CM-based devices, i.e., a superlens and superscatterer, are discussed. From an analysis, the cancellation mechanism of the mismatched CM is studied. In addition, numerical results are provided for illustration. Moreover, further study shows that such cancellation effects might rely on specific conditions. Actually, the conclusions are not restricted to any specific frequencies; they could be extended to many other areas including applications to active cloaking, antennas, and wireless power transfer.

  12. Mammalian mismatch repair

    Pena Diaz, Javier; Jiricny, Josef


    A considerable surge of interest in the mismatch repair (MMR) system has been brought about by the discovery of a link between Lynch syndrome, an inherited predisposition to cancer of the colon and other organs, and malfunction of this key DNA metabolic pathway. This review focuses on recent...... advances in our understanding of the molecular mechanisms of canonical MMR, which improves replication fidelity by removing misincorporated nucleotides from the nascent DNA strand. We also discuss the involvement of MMR proteins in two other processes: trinucleotide repeat expansion and antibody maturation......, in which MMR proteins are required for mutagenesis rather than for its prevention....

  13. PARP-1 enhances the mismatch-dependence of 5′-directed excision in human mismatch repair in vitro

    Liu, Yiyong; Kadyrov, Farid A; Modrich, Paul


    End-directed mismatch-provoked excision has been reconstituted in several purified systems. While 3′-directed excision displays a mismatch dependence similar to that observed in nuclear extracts (≈ 20-fold), the mismatch dependence of 5′-directed excision is only 3 to 4-fold, significantly less than that in extracts (8 to 10-fold). Utilizing a fractionation-based approach, we have isolated a single polypeptide that enhances mismatch dependence of reconstituted 5′-directed excision and have sh...

  14. Mechanisms in E. coli and Human Mismatch Repair (Nobel Lecture).

    Modrich, Paul


    DNA molecules are not completely stable, they are subject to chemical or photochemical damage and errors that occur during DNA replication resulting in mismatched base pairs. Through mechanistic studies Paul Modrich showed how replication errors are corrected by strand-directed mismatch repair in Escherichia coli and human cells. PMID:27198632

  15. Potential for DNA-based identification of Great Lakes fauna: Match and mismatch between taxa inventories and DNA barcode libraries

    DNA-based identification of mixed-organism samples offers the potential to greatly reduce the need for resource-intensive morphological identification, which would be of value both to biotic condition assessment and non-native species early-detection monitoring. However, the abi...

  16. GPS-corrected and GIS-based remapping of the Kalahari Gemsbok National Park and the adjacent area in Botswana

    J. du P. Bothma


    Full Text Available GPS-equipment was used to map the interior roads, major pans and the location of all windmills and solar-equipped boreholes in the Kalahari Gemsbok National Park and the adjacent areas of Botswana. The final map was generated with GIS-equipment, and supplies managers and planners with the first error-free map of the area. The major errors of previous maps are indicated.

  17. Metrology of ground-based satellite validation: co-location mismatch and smoothing issues of total ozone comparisons

    Verhoelst, T.; Granville, J.; Hendrick, F.; Köhler, U.; Lerot, C.; Pommereau, J.-P.; Redondas, A.; Van Roozendael, M.; Lambert, J.-C.


    Comparisons with ground-based correlative measurements constitute a key component in the validation of satellite data on atmospheric composition. The error budget of these comparisons contains not only the measurement errors but also several terms related to differences in sampling and smoothing of the inhomogeneous and variable atmospheric field. A versatile system for Observing System Simulation Experiments (OSSEs), named OSSSMOSE, is used here to quantify these terms. Based on the application of pragmatic observation operators onto high-resolution atmospheric fields, it allows a simulation of each individual measurement, and consequently, also of the differences to be expected from spatial and temporal field variations between both measurements making up a comparison pair. As a topical case study, the system is used to evaluate the error budget of total ozone column (TOC) comparisons between GOME-type direct fitting (GODFITv3) satellite retrievals from GOME/ERS2, SCIAMACHY/Envisat, and GOME-2/MetOp-A, and ground-based direct-sun and zenith-sky reference measurements such as those from Dobsons, Brewers, and zenith-scattered light (ZSL-)DOAS instruments, respectively. In particular, the focus is placed on the GODFITv3 reprocessed GOME-2A data record vs. the ground-based instruments contributing to the Network for the Detection of Atmospheric Composition Change (NDACC). The simulations are found to reproduce the actual measurements almost to within the measurement uncertainties, confirming that the OSSE approach and its technical implementation are appropriate. This work reveals that many features of the comparison spread and median difference can be understood as due to metrological differences, even when using strict co-location criteria. In particular, sampling difference errors exceed measurement uncertainties regularly at most mid- and high-latitude stations, with values up to 10 % and more in extreme cases. Smoothing difference errors only play a role in the

  18. Metrology of ground-based satellite validation: co-location mismatch and smoothing issues of total ozone comparisons

    T. Verhoelst


    Full Text Available Comparisons with ground-based correlative measurements constitute a key component in the validation of satellite data on atmospheric composition. The error budget of these comparisons contains not only the measurement errors but also several terms related to differences in sampling and smoothing of the inhomogeneous and variable atmospheric field. A versatile system for Observing System Simulation Experiments (OSSEs, named OSSSMOSE, is used here to quantify these terms. Based on the application of pragmatic observation operators onto high-resolution atmospheric fields, it allows a simulation of each individual measurement, and consequently, also of the differences to be expected from spatial and temporal field variations between both measurements making up a comparison pair. As a topical case study, the system is used to evaluate the error budget of total ozone column (TOC comparisons between GOME-type direct fitting (GODFITv3 satellite retrievals from GOME/ERS2, SCIAMACHY/Envisat, and GOME-2/MetOp-A, and ground-based direct-sun and zenith–sky reference measurements such as those from Dobsons, Brewers, and zenith-scattered light (ZSL-DOAS instruments, respectively. In particular, the focus is placed on the GODFITv3 reprocessed GOME-2A data record vs. the ground-based instruments contributing to the Network for the Detection of Atmospheric Composition Change (NDACC. The simulations are found to reproduce the actual measurements almost to within the measurement uncertainties, confirming that the OSSE approach and its technical implementation are appropriate. This work reveals that many features of the comparison spread and median difference can be understood as due to metrological differences, even when using strict co-location criteria. In particular, sampling difference errors exceed measurement uncertainties regularly at most mid- and high-latitude stations, with values up to 10 % and more in extreme cases. Smoothing difference errors only

  19. Potential for DNA-based identification of Great Lakes fauna: match and mismatch between taxa inventories and DNA barcode libraries

    Trebitz, Anett S.; Hoffman, Joel C.; Grant, George W.; Billehus, Tyler M.; Pilgrim, Erik M.


    DNA-based identification of mixed-organism samples offers the potential to greatly reduce the need for resource-intensive morphological identification, which would be of value both to bioassessment and non-native species monitoring. The ability to assign species identities to DNA sequences found depends on the availability of comprehensive DNA reference libraries. Here, we compile inventories for aquatic metazoans extant in or threatening to invade the Laurentian Great Lakes and examine the availability of reference mitochondrial COI DNA sequences (barcodes) in the Barcode of Life Data System for them. We found barcode libraries largely complete for extant and threatening-to-invade vertebrates (100% of reptile, 99% of fish, and 92% of amphibian species had barcodes). In contrast, barcode libraries remain poorly developed for precisely those organisms where morphological identification is most challenging; 46% of extant invertebrates lacked reference barcodes with rates especially high among rotifers, oligochaetes, and mites. Lack of species-level identification for many aquatic invertebrates also is a barrier to matching DNA sequences with physical specimens. Attaining the potential for DNA-based identification of mixed-organism samples covering the breadth of aquatic fauna requires a concerted effort to build supporting barcode libraries and voucher collections. PMID:26199185

  20. Nonlinear disturbance observer-based control for multi-input multi-output nonlinear systems subject to mismatching condition

    Yang, Jun; Li, Shihua; Chen, Wen-Hua


    For a multi-input multi-output (MIMO) nonlinear system, the existing disturbance observer-based control (DOBC) only provides solutions to those whose disturbance relative degree (DRD) is higher than or equal to its input relative degree. By designing a novel disturbance compensation gain matrix, a generalised nonlinear DOBC method is proposed in this article to solve the disturbance attenuation problem of the MIMO nonlinear system with arbitrary DRD. It is shown that the disturbances are able to be removed from the output channels by the proposed method with appropriately chosen control parameters. The property of nominal performance recovery, which is the major merit of the DOBCs, is retained with the proposed method. The feasibility and effectiveness of the proposed method are demonstrated by simulation studies of both the numerical and application examples.

  1. Streptococcus mutans counts in plaque adjacent to orthodontic brackets bonded with resin-modified glass ionomer cement or resin-based composite

    Solange Machado Mota


    Full Text Available This study investigated the number of Streptococcus mutans CFU (colony forming units in the saliva and plaque adjacent to orthodontic brackets bonded with a glass ionomer cement - GIC (Fuji Ortho or a resin-based composite - RC (Concise. Twenty male and female patients, aged 12 to 20 years, participated in the study. Saliva was collected before and after placement of appliances. Plaque was collected from areas adjacent to brackets and saliva was again collected on the 15th, 30th, and 45th day after placement. On the 30th day, 0.4% stannous fluoride gel was applied for 4 minutes. No significant modification in the number of Streptococcus mutans CFU in saliva was observed after placement of the fixed orthodontic appliances. On the 15th day, the percentage of Streptococcus mutans CFU in plaque was statistically lower in sites adjacent to GIC-bonded brackets (mean = 0.365 than in those adjacent to RC-bonded brackets (mean = 0.935. No evidence was found of a contribution of GIC to the reduction of CFU in plaque after the 15th day. Topical application of stannous fluoride gel on the 30th day reduced the number of CFU in saliva, but not in plaque. This study suggests that the antimicrobial activity of GIC occurs only in the initial phase and is not responsible for a long-term anticariogenic property.

  2. Heterogenous mismatch-repair status in colorectal cancer

    Joost, Patrick; Veurink, Nynke; Holck, Susanne;


    BACKGROUND: Immunohistochemical staining for mismatch repair proteins is efficient and widely used to identify mismatch repair defective tumors. The tumors typically show uniform and widespread loss of MMR protein staining. We identified and characterized colorectal cancers with alternative......, heterogenous mismatch repair protein staining in order to delineate expression patterns and underlying mechanisms. METHODS: Heterogenous staining patterns that affected at least one of the mismatch repair proteins MLH1, PMS2, MSH2 and MSH6 were identified in 14 colorectal cancers. Based on alternative...... expression patterns macro-dissected and micro-dissected tumor areas were separately analyzed for microsatellite instability and MLH1 promoter methylation. RESULTS: Heterogenous retained/lost mismatch repair protein expression could be classified as intraglandular (within or in-between glandular formations...

  3. Design and analysis of mismatch probes for long oligonucleotide microarrays

    Deng, Ye; He, Zhili; Van Nostrand, Joy D.; Zhou, Jizhong


    Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this approach has not been used for longer oligonucleotide probes. Here, an oligonucleotide microarray was constructed to evaluate and optimize parameters for 50-mer mismatch probe design. A perfect match (PM) and 28 mismatch (MM) probes were designed for each of ten target genes selected from three microorganisms. The microarrays were hybridized with synthesized complementary oligonucleotide targets at different temperatures (e.g., 42, 45 and 50 C). In general, the probes with evenly distributed mismatches were more distinguishable than those with randomly distributed mismatches. MM probes with 3, 4 and 5 mismatched nucleotides were differentiated for 50-mer oligonucleotide probes hybridized at 50, 45 and 42 C, respectively. Based on the experimental data generated from this study, a modified positional dependent nearest neighbor (MPDNN) model was constructed to adjust the thermodynamic parameters of matched and mismatched dimer nucleotides in the microarray environment. The MM probes with four flexible positional mismatches were designed using the newly established MPDNN model and the experimental results demonstrated that the redesigned MM probes could yield more consistent hybridizations. Conclusions: This study provides guidance on the design of MM probes for long oligonucleotides (e.g., 50 mers). The novel MPDNN model has improved the consistency for long MM probes, and this modeling method can potentially be used for the prediction of oligonucleotide microarray hybridizations.

  4. Adjacent segment disease.

    Virk, Sohrab S; Niedermeier, Steven; Yu, Elizabeth; Khan, Safdar N


    EDUCATIONAL OBJECTIVES As a result of reading this article, physicians should be able to: 1. Understand the forces that predispose adjacent cervical segments to degeneration. 2. Understand the challenges of radiographic evaluation in the diagnosis of cervical and lumbar adjacent segment disease. 3. Describe the changes in biomechanical forces applied to adjacent segments of lumbar vertebrae with fusion. 4. Know the risk factors for adjacent segment disease in spinal fusion. Adjacent segment disease (ASD) is a broad term encompassing many complications of spinal fusion, including listhesis, instability, herniated nucleus pulposus, stenosis, hypertrophic facet arthritis, scoliosis, and vertebral compression fracture. The area of the cervical spine where most fusions occur (C3-C7) is adjacent to a highly mobile upper cervical region, and this contributes to the biomechanical stress put on the adjacent cervical segments postfusion. Studies have shown that after fusion surgery, there is increased load on adjacent segments. Definitive treatment of ASD is a topic of continuing research, but in general, treatment choices are dictated by patient age and degree of debilitation. Investigators have also studied the risk factors associated with spinal fusion that may predispose certain patients to ASD postfusion, and these data are invaluable for properly counseling patients considering spinal fusion surgery. Biomechanical studies have confirmed the added stress on adjacent segments in the cervical and lumbar spine. The diagnosis of cervical ASD is complicated given the imprecise correlation of radiographic and clinical findings. Although radiological and clinical diagnoses do not always correlate, radiographs and clinical examination dictate how a patient with prolonged pain is treated. Options for both cervical and lumbar spine ASD include fusion and/or decompression. Current studies are encouraging regarding the adoption of arthroplasty in spinal surgery, but more long

  5. Effects of Mismatched Parameter on Chaotic Synchronization

    PENGJiang-hua; FANGJin-qing


    Chaos-based security communication has become one of the most interesting hot subjects for research of chaotic theory in real world since. In recent years, secure communication via synchronized chaos has been intensely studied. However, in practical application it is difficult to construct two complete identical chaotic systems since there are many reasons to induce parameter mismatch between two systems (response system and drive system).

  6. Robust Control of Urban Industrial Water Mismatching Uncertain System

    LI Kebai


    Full Text Available Urban industrial water system parameter fluctuation producing uncertainty may not occur in a control input channel, can be applied mismatching uncertain system to describe. Based on Lyapunov direct method and linear matrix inequality, design the urban industrial water mismatching uncertain system feedback stabilization robust control scheme. Avoid the defects that the feedback stabilization control method based on the matrix Riccati equation need to preset equation parameters, easier to solve and can reduce the conservative.

  7. The Effect of Basepair Mismatch on DNA Strand Displacement.

    Broadwater, D W Bo; Kim, Harold D


    DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized. Here, we measured toehold-mediated strand displacement kinetics using single-molecule fluorescence in the presence of a single basepair mismatch. The apparent displacement rate varied significantly when the mismatch was introduced in the invading DNA strand. The rate generally decreased as the mismatch in the invader was encountered earlier in displacement. Our data indicate that a single base pair mismatch in the invader stalls branch migration and displacement occurs via direct dissociation of the destabilized incumbent strand from the substrate strand. We combined both branch migration and direct dissociation into a model, which we term the concurrent displacement model, and used the first passage time approach to quantitatively explain the salient features of the observed relationship. We also introduce the concept of splitting probabilities to justify that the concurrent model can be simplified into a three-step sequential model in the presence of an invader mismatch. We expect our model to become a powerful tool to design DNA-based reaction schemes with broad functionality. PMID:27074674

  8. Fast inter-mode decision algorithm for high-efficiency video coding based on similarity of coding unit segmentation and partition mode between two temporally adjacent frames

    Zhong, Guo-Yun; He, Xiao-Hai; Qing, Lin-Bo; Li, Yuan


    High-efficiency video coding (HEVC) introduces a flexible hierarchy of three block structures: coding unit (CU), prediction unit (PU), and transform unit (TU), which have brought about higher coding efficiency than the current national video coding standard H.264/advanced video coding (AVC). HEVC, however, simultaneously requires higher computational complexity than H.264/AVC, although several fast inter-mode decisions were proposed in its development. To further reduce this complexity, a fast inter-mode decision algorithm is proposed based on temporal correlation. Because of the distinct difference of inter-prediction block between HEVC and H.264/AVC, in order to use the temporal correlation to speed up the inter prediction, the correlation of inter-prediction between two adjacent frames needs to be analyzed according to the structure of CU and PU in HEVC. The probabilities of all the partition modes in all sizes of CU and the similarity of CU segmentation and partition modes between two adjacent frames are tested. The correlation of partition modes between two CUs with different sizes in two adjacent frames is tested and analyzed. Based on the characteristics tested and analyzed, at most, two prior partition modes are evaluated for each level of CU, which reduces the number of rate distortion cost calculations. The simulation results show that the proposed algorithm further reduces coding time by 33.0% to 43.3%, with negligible loss in bitrate and peak signal-to-noise ratio, on the basis of the fast inter-mode decision algorithms in current HEVC reference software HM7.0.

  9. Elastic-plastic fracture mechanics of strength-mismatching

    Parks, D.M.; Ganti, S.; McClintock, F.A. [Massachusetts Institute of Technology, Cambridge, MA (United States)


    Approximate solutions to stress-fields are provided for a strength-mismatched interface crack in small-scale yielding (SSY) for non-hardening and low hardening materials. Variations of local deformation intensities, characterized by a J-type contour integral, are proposed. The softer material experiences a higher deformation intensity level, J{sub S}, while the harder material sees a much lower deformation intensity level, J{sub H}, compared to that obtained from the applied J near the respective homogeneous crack-tips. For a low hardening material, the stress fields are obtained by scaling from an elastic/perfectly-plastic problem, based on an effective mismatch, M{sub eff}, which is a function of mismatch, M, and the hardening exponent, n. Triaxial stress build-up is discussed quantitatively in terms of M. The influence of strength-mismatch on cleavage fracture is discussed using Weibull statistics.

  10. Reverse ventilation--perfusion mismatch

    Patients having lobar airway obstruction or consolidation usually have decreases of both ventilation and perfusion on lung scans. We report three patients in whom hypoxic vasoconstriction was apparently incomplete, resulting in a ''reversed'' ventilation-perfusion mismatch. Perfusion of the hypoxic lobe on the radionuclide scan was associated with metabolic alkalosis, pulmonary venous and pulmonary arterial hypertension in these patients

  11. Gordian adjacency for torus knots

    Feller, Peter


    A knot K is called Gordian adjacent to a knot L if there exists an unknotting sequence for L containing K. We provide a sufficient condition for Gordian adjacency of torus knots via the study of knots in the thickened torus. We also completely describe Gordian adjacency for torus knots of index 2 and 3 using Levine-Tristram signatures as obstructions to Gordian adjacency. Finally, Gordian adjacency for torus knots is compared to the notion of adjacency for plane curve singularities.

  12. Seismic structure beneath the Gulf of Aqaba and adjacent areas based on the tomographic inversion of regional earthquake data

    El Khrepy, Sami; Koulakov, Ivan; Al-Arifi, Nassir; Petrunin, Alexey G.


    We present the first 3-D model of seismic P and S velocities in the crust and uppermost mantle beneath the Gulf of Aqaba and surrounding areas based on the results of passive travel time tomography. The tomographic inversion was performed based on travel time data from ˜ 9000 regional earthquakes provided by the Egyptian National Seismological Network (ENSN), and this was complemented with data from the International Seismological Centre (ISC). The resulting P and S velocity patterns were generally consistent with each other at all depths. Beneath the northern part of the Red Sea, we observed a strong high-velocity anomaly with abrupt limits that coincide with the coastal lines. This finding may indicate the oceanic nature of the crust in the Red Sea, and it does not support the concept of gradual stretching of the continental crust. According to our results, in the middle and lower crust, the seismic anomalies beneath the Gulf of Aqaba seem to delineate a sinistral shift (˜ 100 km) in the opposite flanks of the fault zone, which is consistent with other estimates of the left-lateral displacement in the southern part of the Dead Sea Transform fault. However, no displacement structures were visible in the uppermost lithospheric mantle.

  13. Mismatch Negativity: Translating the Potential



    The mismatch negativity (MMN) component of the auditory event-related potential has become a valuable tool in cognitive neuroscience. Its reduced size in persons with schizophrenia is of unknown origin but theories proposed include links to problems in experience-dependent plasticity reliant on N-methyl-d-aspartate glutamate receptors. In this review we address the utility of this tool in revealing the nature and time course of problems in perceptual inference in this illness together with it...

  14. Educational Mismatch and Wait Unemployment

    Patrizia Ordine; Giuseppe Rose


    This work investigates educational mismatch and its interrelationships with individual unemployment duration. By studying unemployment histories of Italian workers we show that overeducated have longer unemployment spells than well matched workers. Using duration models we show that hazard rates of graduates are higher than those of undergraduates only for transitions toward occupations that require the competencies provided by the universities. This process is strictly related to innate abil...

  15. Even aberration measurement of lithographic projection optics based on intensity difference of adjacent peaks in alternating phase-shifting mask image

    We propose an in situ technique for measuring an even aberration of lithographic projection optics. By using the Hopkins theory of partially coherent imaging and the thick-mask model, the linear relationship between the intensity difference of adjacent peaks in an alternating phase-shifting mask image and an even aberration is established by equations and verified by numerical results. The sensitivity of measuring the even aberration of lithographic projection optics based on this linear relationship is analyzed, and the measurement mark is designed accordingly. Measurement performance of the present technique is evaluated using the lithographic simulator PROLITH, which shows that the present technique is capable of measuring the even aberration of lithographic projection optics with ultrahigh measurement accuracy.

  16. $p$-th Clustering coefficients and $q$-$th$ degrees of separation based on String-Adjacent Formulation

    Toyota, Norihito


    The phenomenon of six degrees of separation is an old but attractive subject. The deep understanding has been uncovered yet, especially how closed paths included in a network affect six degrees of separation are an important subject left yet. For it, some researches have been made\\cite{Newm21}, \\cite{Aoyama}. Recently we have develop a formalism \\cite{Toyota3},\\cite{Toyota4} to explore the subject based on the string formalism developed by Aoyama\\cite{Aoyama}. The formalism can systematically investigate the effect of closed paths, especially generalized clustering coefficient $C_{(p)}$ introduced in \\cite{Toyota4}, on six degrees of separation. In this article, we analyze general $q$-th degrees of separation by using the formalism developed by us. So we find that the scale free network with exponent $\\gamma=3$ just display six degrees of separation. Furthermore we drive a phenomenological relation between the separation number $q$ and $C_{(p)}$ that has crucial information on circle structures in networks.

  17. Chimeric Proteins to Detect DNA Damage and Mismatches

    McCutchen-Maloney, S; Malfatti, M; Robbins, K M


    The goal of this project was to develop chimeric proteins composed of a DNA mismatch or damage binding protein and a nuclease, as well as methods to detect DNA mismatches and damage. We accomplished this through protein engineering based on using polymerase chain reactions (PCRs) to create chimeras with novel functions for damage and mismatch detection. This project addressed fundamental questions relating to disease susceptibility and radiation-induced damage in cells. It also supported and enhanced LLNL's competency in the emerging field of proteomics. In nature, DNA is constantly being subjected to damaging agents such as exposure to ultraviolet (UV) radiation and various environmental and dietary carcinogens. If DNA damage is not repaired however, mutations in DNA result that can eventually manifest in cancer and other diseases. In addition to damage-induced DNA mutations, single nucleotide polymorphisms (SNPs), which are variations in the genetic sequence between individuals, may predispose some to disease. As a result of the Human Genome Project, the integrity of a person's DNA can now be monitored. Therefore, methods to detect DNA damage, mutations, and SNPs are useful not only in basic research but also in the health and biotechnology industries. Current methods of detection often use radioactive labeling and rely on expensive instrumentation that is not readily available in many research settings. Our methods to detect DNA damage and mismatches employ simple gel electrophoresis and flow cytometry, thereby alleviating the need for radioactive labeling and expensive equipment. In FY2001, we explored SNP detection by developing methods based on the ability of the chimeric proteins to detect mismatches. Using multiplex assays with flow cytometry and fluorescent beads to which the DNA substrates where attached, we showed that several of the chimeras possess greater affinity for damaged and mismatched DNA than for native DNA. This affinity was

  18. A Compression Method for ECG Based on DCT of Difference between Two Adjacent Frames%帧间差值的DCT压缩ECG数据的方法

    王培康; 费小英


    心电图(ECG)信号具有准周期性,各个心动周期(称其为帧)的ECG波形极为相似,将相邻两帧的ECG波形数据相减,得到差值数据的波形比原始ECG波形平坦、数据值小,差值数据的DCT有效分量更为集中在低频段,且这些分量的幅度较低,由此,可以得到更高的数据压缩比。因此本文提出了一种利用ECG帧间差值代替原始ECG信号进行DCT变换,并用双码长编码方法对DCT分量进行编码的数据压缩方法。用该方法得到的重构ECG波形具有良好的保真度。%Since most of the DCT coefficients of an initial ECG signal are spread in low frequency,so is the energy of the signal, we can get a higher compression ratio (CR) by distributing DCT coefficients in a smaller region of low frequency and by degrading the amplitude of main DCT coefficients. ECG is a quasi-periodic signal with strong correlation between adjacent frames, so the difference sequence between them is more smooth and has lower amplitudes. Based on this property, a method of ECG data compression via DCT is presented in this paper, and it replaces the DCT coefficients of the original signal with the DCT coefficients of difference between two adjacent frames of the original signal and the DCT coefficients are encoded into two kinds of codes with different lengths. The quality of the reconstructed signal got by this way is high.

  19. Seasonal changes in phytoplankton biomass and dominant species in the Changjiang River Estuary and adjacent seas: General trends based on field survey data 1959-2009

    Yang, Shu; Han, Xiurong; Zhang, Chuansong; Sun, Baiye; Wang, Xiulin; Shi, Xiaoyong


    The characteristics of seasonal variation in phytoplankton biomass and dominant species in the Changjiang River Estuary and adjacent seas were discussed based on field investigation data from 1959 to 2009. The field data from 1981 to 2004 showed that the Chlorophyll- a concentration in surface seawater was between 0.4 and 8.5 μg dm-3. The seasonal changes generally presented a bimodal trend, with the biomass peaks occurring in May and August, and Chlorophyll-a concentration was the lowest in winter. Seasonal biomass changes were mainly controlled by temperature and nutrient levels. From the end of autumn to the next early spring, phytoplankton biomass was mainly influenced by temperature, and in other seasons, nutrient level (including the nutrient supply from the terrestrial runoffs) was the major influence factor. Field investigation data from 1959 to 2009 demonstrated that diatoms were the main phytoplankton in this area, and Skeletonema costatum, Pseudo-nitzschia pungens, Coscinodiscus oculus-iridis, Thalassinoema nitzschioides, Paralia sulcata, Chaetoceros lorenzianus, Chaetoceros curvisetus, and Prorocentrum donghaiense Lu were common dominant species. The seasonal variations in major dominant phytoplankton species presented the following trends: 1) Skeletonema (mainly S. costatum) was dominant throughout the year; and 2) seasonal succession trends were Coscinodiscus (spring) → Chaetoceros (summer and autumn) → Coscinodiscus (winter). The annual dominance of S. costatum was attributed to its environmental eurytopicity and long standing time in surface waters. The seasonal succession of Coscinodiscus and Chaetoceros was associated with the seasonal variation in water stability and nutrient level in this area. On the other hand, long-term field data also indicated obvious interannual variation of phytoplankton biomass and community structure in the Changjiang River Estuary and adjacent seas: average annual phytoplankton biomass and dinoflagellate

  20. Ecological mapping and data quality assessment for the needs of ecosystem-based marine spatial management: case study Greek Ionian Sea and the adjacent gulfs



    Full Text Available Mapping of ecosystem components (natural and socioeconomic is a prerequisite for ecosystem-based marine spatial management (EB-MSM. To initiate the process of EB-MSM in the Greek Ionian Sea and the adjacent gulfs, the main relevant ecosystem components were mapped based on existing spatial information and expert judgment. The natural components mapped included habitat types and species targeted for conservation, according to national and European legislation and international agreements. Main human activities/pressures related to fisheries, aquaculture, tourism, and industry were also mapped. In order to assess the quality of data used to map ecosystem components and therefore take into consideration the inherent uncertainty, an assessment of 5 semi-quantitative data indicators based on a pedigree matrix was conducted. Through this qualitative approach we gained information related to the sources, acquisition and verification procedures, statistical properties, and temporal & geographical correlation, along with the collection process quality of the ecosystem components under study. A substantial overlapping between ecological features and human activities was identified, confirming the need for a well-planned approach to marine space management, in order to mitigate conflicts for marine resources and conserve marine ecosystems and their associated goods and services.

  1. Fabricating Hexagonal Al-Doped LiCoO2 Nanomeshes Based on Crystal-Mismatch Strategy for Ultrafast Lithium Storage.

    Xu, Hai-Tao; Zhang, Huijuan; Liu, Li; Feng, Yangyang; Wang, Yu


    In the designed synthesis, low crystal-mismatch strategy has been applied in the synthesis of ion-doped LiCoO2 materials, and a good success of single crystal property has been achieved between the precursor and the final sample for the first time. The hexagonal LiCo0.8Al0.26O2 (LCAO) nanomesh possesses several advantages in morphology and crystal structure, including mesoporous structure, single crystal, atomic even distribution, high exposing surface area as (100) or their equivalent planes, and shortened Li ions diffusion distance. All the merits are beneficial to the application in Li-ion batteries (LIBs) cathode, for example, accelerating Li ions diffusion rate, improving the Li ions shuttle between the LCAO nanomesh and electrolyte, and reducing the Li ions capacitive behavior during Li intercalation. Hence, our research adopts Al-contained precursor with morphology of hexagonal nanoplates to fabricate designed Al-doped LiCoO2 nanomeshes and greatly improves the cathode performance in LIBs. PMID:26333181

  2. Preliminary Studies on Base Substitutions and Repair of DNA Mismatch Damage Stimulated by Low Energy N+ Ion Beam Implantation in Escherichia coli

    谢传晓; 郭金华; 程备久; 余增亮


    Ever since the low energy N+ ion beam has been accepted that the mutation effectsof ionizing radiation are attributed mainly to direct or indirect damage to DNA. Evidences basedon naked DNA irradiation in support of a mutation spectrum appears to be consistent, but directproof of such results in vivo are limited. Using mutS, dam and/or dcm defective Eschericha colimutator strains, an preliminary experimental system on induction of in vivo mutation spectra oflow energy N+ ion beam has been established in this study. It was observed that the mutationrates of rifampicin resistance induced by N+ implantation were quite high, ranging from 9.2 ×10-8 to 4.9 × 10-5 at the dosage of 5.2 × 1014 ions/cm2. Strains all had more than 90-fold highermutation rate than its spontaneous mutation rate determined by this method. It reveals thatbase substitutions involve in induction of mutation of low energy nitrogen ion beam implantation.The mutation rates of mutator strains were nearly 500-fold (GM2929), 400-fold (GM5864) and6-fold larger than that of AB1157. The GM2929 and GM5864 both lose the ability of repair DNAmismatch damage by virtue of both dam and dcm pathways defective (GM2929) or failing toassemble the repair complex (GM5864) respectively. It may explain the both strains had a similarhigher mutation rate than GM124 did. It indicated that DNA cytosine methylase might play animportant role in mismatch repair of DNA damage induced by N+ implantation. The furtherrelated research were also discussed.

  3. High fitness costs of climate change-induced camouflage mismatch.

    Zimova, Marketa; Mills, L Scott; Nowak, J Joshua


    Anthropogenic climate change has created myriad stressors that threaten to cause local extinctions if wild populations fail to adapt to novel conditions. We studied individual and population-level fitness costs of a climate change-induced stressor: camouflage mismatch in seasonally colour molting species confronting decreasing snow cover duration. Based on field measurements of radiocollared snowshoe hares, we found strong selection on coat colour molt phenology, such that animals mismatched with the colour of their background experienced weekly survival decreases up to 7%. In the absence of adaptive response, we show that these mortality costs would result in strong population-level declines by the end of the century. However, natural selection acting on wide individual variation in molt phenology might enable evolutionary adaptation to camouflage mismatch. We conclude that evolutionary rescue will be critical for hares and other colour molting species to keep up with climate change. PMID:26799459

  4. Mismatch repair deficiency testing in clinical practice.

    Buza, Natalia; Ziai, James; Hui, Pei


    Lynch syndrome, an autosomal dominant inherited disorder, is caused by inactivating mutations involving DNA mismatch repair (MMR) genes. This leads to profound genetic instability, including microsatellite instability (MSI) and increased risk for cancer development, particularly colon and endometrial malignancies. Clinical testing of tumor tissues for the presence of MMR gene deficiency is standard practice in clinical oncology, with immunohistochemistry and PCR-based microsatellite instability analysis used as screening tests to identify potential Lynch syndrome families. The ultimate diagnosis of Lynch syndrome requires documentation of mutation within one of the four MMR genes (MLH1, PMS2, MSH2 and MSH6) or EPCAM, currently achieved by comprehensive sequencing analysis of germline DNA. In this review, the genetic basis of Lynch syndrome, methodologies of MMR deficiency testing, and current diagnostic algorithms in the clinical management of Lynch syndrome, are discussed. PMID:26895074

  5. Metamer mismatching in practice versus theory.

    Zhang, Xiandou; Funt, Brian; Mirzaei, Hamidreza


    Metamer mismatching (the phenomenon that two objects matching in color under one illuminant may not match under a different illuminant) potentially has important consequences for color perception. Logvinenko et al. [PLoS ONE10, e0135029 (2015)] show that in theory the extent of metamer mismatching can be very significant. This paper examines metamer mismatching in practice by computing the volumes of the empirical metamer mismatch bodies and comparing them to the volumes of the theoretical mismatch bodies. A set of more than 25 million unique reflectance spectra is assembled using datasets from several sources. For a given color signal (e.g., CIE XYZ) recorded under a given first illuminant, its empirical metamer mismatch body for a change to a second illuminant is computed as follows: the reflectances having the same color signal when lit by the first illuminant (i.e., reflect metameric light) are computationally relit by the second illuminant, and the convex hull of the resulting color signals then defines the empirical metamer mismatch body. The volume of these bodies is shown to vary systematically with Munsell value and chroma. The empirical mismatch bodies are compared to the theoretical mismatch bodies computed using the algorithm of Logvinenko et al. [IEEE Trans. Image Process.23, 34 (2014)]. There are three key findings: (1) the empirical bodies are found to be substantially smaller than the theoretical ones; (2) the sizes of both the empirical and theoretical bodies show a systematic variation with Munsell value and chroma; and (3) applied to the problem of color-signal prediction, the centroid of the empirical metamer mismatch body is shown to be a better predictor of what a given color signal might become under a specified illuminant than state-of-the-art methods. PMID:26974929

  6. A GIS-based methodology to quantitatively define an Adjacent Protected Area in a shallow karst cavity: the case of Altamira cave.

    Elez, J; Cuezva, S; Fernandez-Cortes, A; Garcia-Anton, E; Benavente, D; Cañaveras, J C; Sanchez-Moral, S


    Different types of land use are usually present in the areas adjacent to many shallow karst cavities. Over time, the increasing amount of potentially harmful matter and energy, of mainly anthropic origin or influence, that reaches the interior of a shallow karst cavity can modify the hypogeal ecosystem and increase the risk of damage to the Palaeolithic rock art often preserved within the cavity. This study proposes a new Protected Area status based on the geological processes that control these matter and energy fluxes into the Altamira cave karst system. Analysis of the geological characteristics of the shallow karst system shows that direct and lateral infiltration, internal water circulation, ventilation, gas exchange and transmission of vibrations are the processes that control these matter and energy fluxes into the cave. This study applies a comprehensive methodological approach based on Geographic Information Systems (GIS) to establish the area of influence of each transfer process. The stratigraphic and structural characteristics of the interior of the cave were determined using 3D Laser Scanning topography combined with classical field work, data gathering, cartography and a porosity-permeability analysis of host rock samples. As a result, it was possible to determine the hydrogeological behavior of the cave. In addition, by mapping and modeling the surface parameters it was possible to identify the main features restricting hydrological behavior and hence direct and lateral infiltration into the cave. These surface parameters included the shape of the drainage network and a geomorphological and structural characterization via digital terrain models. Geological and geomorphological maps and models integrated into the GIS environment defined the areas involved in gas exchange and ventilation processes. Likewise, areas that could potentially transmit vibrations directly into the cave were identified. This study shows that it is possible to define a

  7. Modeling of channel mismatch in time-interleaved SAR ADC

    Dengquan, Li; Liang, Zhang; Zhangming, Zhu; Yintang, Yang


    In a time-interleaved analog-to-digital converter (TI ADC), several individual ADCs operate in parallel to achieve a higher sampling rate. Low power consumption as well as good linearity can be obtained by applying successive approximation register (SAR) converters as sub-channel ADCs. In spite of the advantages, this structure suffers from three mismatches, which are offset mismatch, gain mismatch, and time skew. This paper focuses on a TI SAR ADC with a number of channels. The mismatch effects in the frequency domain are analyzed and the derived close form formulas are verified based on Matlab. In addition, we clarify that the standard deviation of DNL and INL of an M-channel TI ADC is reduced by a factor of \\sqrt M compared to a single channel ADC. The formulas can be used to derive the corresponding requirements when designing a TI ADC. Our analysis process is able to inform the study of calibration algorithms. Project supported by the National Natural Science Foundation of China (Nos. 61234002, 61322405, 61306044, 61376033) and the National High-Tech Program of China (No. 2013AA014103).

  8. DNA Mismatch Repair and Oxidative DNA Damage: Implications for Cancer Biology and Treatment

    Many components of the cell, including lipids, proteins and both nuclear and mitochondrial DNA, are vulnerable to deleterious modifications caused by reactive oxygen species. If not repaired, oxidative DNA damage can lead to disease-causing mutations, such as in cancer. Base excision repair and nucleotide excision repair are the two DNA repair pathways believed to orchestrate the removal of oxidative lesions. However, recent findings suggest that the mismatch repair pathway may also be important for the response to oxidative DNA damage. This is particularly relevant in cancer where mismatch repair genes are frequently mutated or epigenetically silenced. In this review we explore how the regulation of oxidative DNA damage by mismatch repair proteins may impact on carcinogenesis. We discuss recent studies that identify potential new treatments for mismatch repair deficient tumours, which exploit this non-canonical role of mismatch repair using synthetic lethal targeting

  9. Compensation of IQ mismatch in optical PDM-OFDM coherent receivers

    Chung, Hwan Seok; Chang, Sun Hyok; Kim, Kwangjoon


    The performance enhancements based on Gram-Schmidt orthogonalization procedure (GSOP) for compensating IQ mismatch in coherent optical orthogonal frequency division multiplexing (CO-OFDM) systems are investigated. We analytically explore IQ mismatch in optical OFDM systems and investigate the impacts of phase and amplitude IQ mismatch on required optical signal-to-noise ratio (OSNR) for the different values of data mapping and polarization multiplexing. The impacts of analog-to-digital converter (ADC) resolution and the number of samples in GSOP are also evaluated. The results show that the GSOP operation efficiently compensate IQ mismatch induced performance degradations regardless of the amount of IQ phase mismatch, density of data mapping, and polarization multiplexing.

  10. DNA Mismatch Repair and Oxidative DNA Damage: Implications for Cancer Biology and Treatment

    Bridge, Gemma; Rashid, Sukaina; Martin, Sarah A., E-mail: [Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ (United Kingdom)


    Many components of the cell, including lipids, proteins and both nuclear and mitochondrial DNA, are vulnerable to deleterious modifications caused by reactive oxygen species. If not repaired, oxidative DNA damage can lead to disease-causing mutations, such as in cancer. Base excision repair and nucleotide excision repair are the two DNA repair pathways believed to orchestrate the removal of oxidative lesions. However, recent findings suggest that the mismatch repair pathway may also be important for the response to oxidative DNA damage. This is particularly relevant in cancer where mismatch repair genes are frequently mutated or epigenetically silenced. In this review we explore how the regulation of oxidative DNA damage by mismatch repair proteins may impact on carcinogenesis. We discuss recent studies that identify potential new treatments for mismatch repair deficient tumours, which exploit this non-canonical role of mismatch repair using synthetic lethal targeting.

  11. Conformational arm-wrestling: battles for stereochemical control in benzamides bearing matched and mismatched chiral 2- and 6-substituents.

    Clayden, Jonathan; Foricher, Yann J Y; Helliwell, Madeleine; Johnson, Paul; Mitjans, David; Vinader, Victoria


    The orientation of a tertiary amide group adjacent to an aromatic ring may be governed by the stereochemistry of an adjacent chiral substituent. With a chiral substituent in both ortho positions, matched/mismatched pairs of isomers result. Evidence for matched stereochemistry is provided by the clean NMR spectra of single conformers, while mismatching gives poor or unexpected selectivities in the formation of chiral substituents, or mixtures of amide conformers. Attempts to use the match-mismatch effect to select for racemic pairs of enantiomeric substituents, and hence develop a "racemate-sequestering" reagent, are described, along with the use of "matching" to scavenge a single enantiomer of a diamine from material of incomplete enantiomeric purity. PMID:16446801

  12. Mismatch repair and treatment resistance in ovarian cancer

    van der Burg Maria EL; Ewing Patricia C; Ritstier Kirsten; van Kuijk Patricia F; Dinjens Winand NM; van Staveren Iris L; Helleman Jozien; Stoter Gerrit; Berns Els MJJ


    Abstract Background The treatment of ovarian cancer is hindered by intrinsic or acquired resistance to platinum-based chemotherapy. The aim of this study is to determine the frequency of mismatch repair (MMR) inactivation in ovarian cancer and its association with resistance to platinum-based chemotherapy. Methods We determined, microsatellite instability (MSI) as a marker for MMR inactivation (analysis of BAT25 and BAT26), MLH1 promoter methylation status (methylation specific PCR on bisulfi...


    Hatos Roxana


    Full Text Available Transition from school to work is a main issue with many fields of study. Studies on transition from school to work, have highlight the importance of two categories of factors at the level of the individual formal proceedings which may affect how easy it is to graduate to integrate into the labor market: 1 so far as the educational systems are transmitting specific competences as compared with those general and 2 so far as there are direct links between employers and the education system. In this way, are reduced the costs of selection and allocation for employers. A poor articulation between educational institutions and the labor market produce a high level of unmatched competences of assimilated by formal education and competencies required of the labor market (skill mismatch (Parodi et al., 2012. The surveys with European employers reflect particular difficulties that they are experiencing in employment vacancies. Investigation on the European companies in the spring of 2013 found that 40% of the firms in the EU have difficulty in finding employees with suitable qualification (CEDEFOP-European Center for the Development of the Vocational Training, 2014. Skills mismatch is a generic term that refers to various types of imbalances between skills and competences offered and those required in the labor market. Concept has become one intensely discussed and submitted to measurement in international research on the background concerns the under-utilization human resource. Numerous opinion polls with employers come to the same unexpected conclusion - that despite high unemployment many posts can't find occupants satisfactorily prepared and identify the causes: most of them criticized the lack of skills of the candidates or the absence of skills specific to the workplace. Based on the latest studies on international databases have built a set of questions that, through secondary analysis, we tried to find answers. Questions that we try to give answer

  14. Mismatch Calibration in LINC Power Amplifiers Using Modified Gradient Algorithm

    Hosein Miar-Naimi


    Full Text Available One of the power amplifiers linearization technique is linear amplification with nonlinear components (LINC.The effects of phase and gain imbalances between two signal branches in LINC transmitters have been analyzed in this paper. Then a feedback path has been added to compensate this mismatches, using two complex gain in each path.This complex gains are controlled in a way to calibrate any gain and phase mismatches between two path using Modified Gradient Algorithm (MGA adaptively. The main advantages of this algorithm over other algorithms are zero residual error and fast convergence time. In the proposedarchitecture power amplifiers in each path are modeled as a complex gain which its phase and amplitude depend on input signal level. Many simulations have been performed to validate the proposed self calibrating LINC transmitter. Simulation results have confirmed the analyticalpredictions. According to simulation results the proposed structure has around 40 dB/Hz improvement in the first adjacent channel of the output signal spectrum.

  15. Improved Dijkstra Algorithm Based on the Adjacent Point in Shortest Path%基于邻接点算法的Dijkstra优化研究



    Abstract the urban road into network diagram and definition of data structures for road and site. Improve the Dijkstra by adjacent point algorithm, the method build the adjacency matrix and the initial judgment matrix and get the shortest path between two points in the network diagram. The efficiency of the algorithm is apparent when there are more points. Experiments show that adjacent point algorithm not only saves storage space, but also improves the efficiency of operations.%在将城市道路实体抽象为图论理论中的网络图,对道路和站点数据结构进行定义的基础上,利用邻接点算法对Dijkstra算法进行改进,通过构建邻接矩阵和初始判断矩阵,得出网络图中任意两点间的最短路径.当点数越多时改进算法的效率就显现出来,结果表明邻接点算法在一定程度上既节省了存储空间,又提高了运算效率.

  16. Wavelength mismatch effect in electromagnetically induced absorption

    Bharti, Vineet; Wasan, Ajay; Natarajan, Vasant


    We present a theoretical investigation of the phenomenon of electromagnetically induced absorption (EIA) in a 4-level system consisting of vee and ladder subsystems. The four levels are coupled using one weak probe field, and two strong control fields. We consider an experimental realization using energy levels of Rb. This necessitates dealing with different conditions of wavelength mismatch---near-perfect match where all three wavelengths are approximately equal; partial mismatch where the w...

  17. Migrant Educational Mismatch and the Labour Market

    Piracha, Matloob; Vadean, Florin


    This paper reviews the literature on educational mismatch of immigrants in the labour market of destination countries. It draws on the theoretical arguments postulated in the labour economics literature and discusses their extension in the analysis of the causes and effects of immigrants' educational mismatch in the destination country. Relevant empirical approaches have been presented which show that immigrants are in general more over-educated than natives and that the reasons for those ran...

  18. Ventromedial prefrontal cortex drives hippocampal theta oscillations induced by mismatch computations.

    Garrido, Marta I; Barnes, Gareth R; Kumaran, Dharshan; Maguire, Eleanor A; Dolan, Raymond J


    Detecting environmental change is fundamental for adaptive behavior in an uncertain world. Previous work indicates the hippocampus supports the generation of novelty signals via implementation of a match-mismatch detector that signals when an incoming sensory input violates expectations based on past experience. While existing work has emphasized the particular contribution of the hippocampus, here we ask which other brain structures also contribute to match-mismatch detection. Furthermore, we leverage the fine-grained temporal resolution of magnetoencephalography (MEG) to investigate whether mismatch computations are spectrally confined to the theta range, based on the prominence of this range of oscillations in models of hippocampal function. By recording MEG activity while human subjects perform a task that incorporates conditions of match-mismatch novelty we show that mismatch signals are confined to the theta band and are expressed in both the hippocampus and ventromedial prefrontal cortex (vmPFC). Effective connectivity analyses (dynamic causal modeling) show that the hippocampus and vmPFC work as a functional circuit during mismatch detection. Surprisingly, our results suggest that the vmPFC drives the hippocampus during the generation and processing of mismatch signals. Our findings provide new evidence that the hippocampal-vmPFC circuit is engaged during novelty processing, which has implications for emerging theories regarding the role of vmPFC in memory. PMID:26187453

  19. Asymmetric response of demand-supply mismatch to investor's sentiment

    Marcato, Gianluca; Nanda, Anupam


    We look through both the demand and supply side information to understand dynamics of price determination in the real estate market and examine how accurately investors’ attitudes predict the market returns and thereby flagging off extent of any demand-supply mismatch. Our hypothesis is based on the possibility that investors’ call for action in terms of their buy/sell decision and adjustment in reservation/offer prices may indicate impending demand-supply imbalances in the market. In the pro...


    Enrico Marelli; Dario Sciulli; Marcello Signorelli


    In this paper we first review the (potential and actual) role of the Universities for the local economies in which they operate, especially considering the implications deriving from the degree of skill mismatch (over-education) in a local labour market. Then, in the second part of the paper, we realise an empirical investigation based on administrative information of an Italian University matched with the data of the job centres of the local (provincial) labour market in order to...

  1. Nonspinning searches for spinning binaries in ground-based detector data: Amplitude and mismatch predictions in the constant precession cone approximation

    Brown, D; O'Shaughnessy, R


    Current searches for compact binary mergers by ground-based gravitational-wave detectors assume for simplicity the two bodies are not spinning. If the binary contains compact objects with significant spin, then this can reduce the sensitivity of these searches, particularly for black hole--neutron star binaries. In this paper we investigate the effect of neglecting precession on the sensitivity of searches for spinning binaries using non-spinning waveform models. We demonstrate that in the sensitive band of Advanced LIGO, the angle between the binary's orbital angular momentum and its total angular momentum is approximately constant. Under this \\emph{constant precession cone} approximation, we show that the gravitational-wave phasing is modulated in two ways: a secular increase of the gravitational-wave phase due to precession and an oscillation around this secular increase. We show that this secular evolution occurs in precisely three ways, corresponding to physically different apparent evolutions of the bin...

  2. In Situ Measurement of the γ/ γ' Lattice Mismatch Evolution of a Nickel-Based Single-Crystal Superalloy During Non-isothermal Very High-Temperature Creep Experiments

    Le Graverend, Jean-Briac; Dirand, Laura; Jacques, Alain; Cormier, Jonathan; Ferry, Olivier; Schenk, Thomas; Gallerneau, Franck; Kruch, Serge; Mendez, José


    The evolution of the γ/ γ' lattice mismatch of the AM1 single-crystal superalloy was measured during in situ non-isothermal very high-temperature creep tests under X-ray synchrotron radiation. The magnitude of the effective lattice mismatch in the 1273 K to 1323 K (1000 °C to 1050 °C) temperature range always increased after overheatings performed at temperatures lower than 1403 K (1130 °C). In contrast, a decrease of its magnitude was observed after overheatings at temperatures greater than 1453 K (1180 °C) due to massive dislocation recovery processes occurring at very high temperature.

  3. Fuzzy Backstepping Sliding Mode Control for Mismatched Uncertain System

    H. Q. Hou


    Full Text Available Sliding mode controllers have succeeded in many control problems that the conventional control theories have difficulties to deal with; however it is practically impossible to achieve high-speed switching control. Therefore, in this paper an adaptive fuzzy backstepping sliding mode control scheme is derived for mismatched uncertain systems. Firstly fuzzy sliding mode controller is designed using backstepping method based on the Lyapunov function approach, which is capable of handling mismatched problem. Then fuzzy sliding mode controller is designed using T-S fuzzy model method, it can improve the performance of the control systems and their robustness. Finally this method of control is applied to nonlinear system as a case study; simulation results are also provided the performance of the proposed controller.

  4. Wavelength mismatch effect in electromagnetically induced absorption

    Bharti, Vineet; Wasan, Ajay; Natarajan, Vasant


    We present a theoretical investigation of the phenomenon of electromagnetically induced absorption (EIA) in a 4-level system consisting of vee and ladder subsystems. The four levels are coupled using one weak probe field, and two strong control fields. We consider an experimental realization using energy levels of Rb. This necessitates dealing with different conditions of wavelength mismatch-near-perfect match where all three wavelengths are approximately equal; partial mismatch where the wavelength of one control field is less than the other fields; and complete mismatch where all three wavelengths are unequal. We present probe absorption profiles with Doppler averaging at room temperature to account for experiments in a room temperature Rb vapor cell. Our analysis shows that EIA resonances can be studied using Rydberg states excited with diode lasers.

  5. Wavelength mismatch effect in electromagnetically induced absorption

    Bharti, Vineet; Natarajan, Vasant


    We present a theoretical investigation of the phenomenon of electromagnetically induced absorption (EIA) in a 4-level system consisting of vee and ladder subsystems. The four levels are coupled using one weak probe field, and two strong control fields. We consider an experimental realization using energy levels of Rb. This necessitates dealing with different conditions of wavelength mismatch---near-perfect match where all three wavelengths are approximately equal; partial mismatch where the wavelength of one control field is less than the other fields; and complete mismatch where all three wavelengths are unequal. We present probe absorption profiles with Doppler averaging at room temperature to account for experiments in a room temperature Rb vapor cell. Our analysis shows that EIA resonances can be studied using Rydberg states excited with diode lasers.

  6. Mismatch and noise in modern IC processes

    Marshall, Andrew


    Component variability, mismatch, and various noise effects are major contributors to design limitations in most modern IC processes. Mismatch and Noise in Modern IC Processes examines these related effects and how they affect the building block circuits of modern integrated circuits, from the perspective of a circuit designer.Variability usually refers to a large scale variation that can occur on a wafer to wafer and lot to lot basis, and over long distances on a wafer. This phenomenon is well understood and the effects of variability are included in most integrated circuit design with the use

  7. A new interpretation of deformation rates in the Snake River Plain and adjacent basin and range regions based on GPS measurements

    S.J. Payne; R. McCaffrey; R.W. King; S.A. Kattenhorn


    We evaluate horizontal Global Positioning System (GPS) velocities together with geologic, volcanic, and seismic data to interpret extension, shear, and contraction within the Snake River Plain and the Northern Basin and Range Province, U.S.A. We estimate horizontal surface velocities using GPS data collected at 385 sites from 1994 to 2009 and present an updated velocity field within the Stable North American Reference Frame (SNARF). Our results show an ENE-oriented extensional strain rate of 5.9 {+-} 0.7 x 10{sup -9} yr{sup -1} in the Centennial Tectonic belt and an E-oriented extensional strain rate of 6.2 {+-} 0.3 x 10{sup -9} yr{sup -1} in the Intermountain Seismic belt combined with the northern Great Basin. These extensional strain rates contrast with the regional north-south contraction of -2.6 {+-} 1.1 x 10{sup -9} yr{sup -1} calculated in the Snake River Plain and Owyhee-Oregon Plateau over a 125 x 650 km region. Tests that include dike-opening reveal that rapid extension by dike intrusion in volcanic rift zones does not occur in the Snake River Plain at present. This slow internal deformation in the Snake River Plain is in contrast to the rapidly-extending adjacent Basin and Range provinces and implies shear along boundaries of the Snake River Plain. We estimate right-lateral shear with slip rates of 0.5-1.5 mm/yr along the northwestern boundary adjacent to the Centennial Tectonic belt and left-lateral oblique extension with slip rates of <0.5 to 1.7 mm/yr along the southeastern boundary adjacent to the Intermountain Seismic belt. The fastest lateral shearing occurs near the Yellowstone Plateau where strike-slip focal mechanisms and faults with observed strike-slip components of motion are documented. The regional GPS velocity gradients are best fit by nearby poles of rotation for the Centennial Tectonic belt, Idaho batholith, Snake River Plain, Owyhee-Oregon Plateau, and central Oregon, indicating that clockwise rotation is driven by extension to the

  8. Nonequilibrium phenomena in adjacent electrically isolated nanostructures

    Khrapai, V. S.; Ludwig, S.; Kotthaus, J. P.; Tranitz, H. P.; Wegscheider, W.


    We report on nonequilibrium interaction phenomena between adjacent but electrostatically separated nanostructures in GaAs. A current flowing in one externally biased nanostructure causes an excitation of electrons in a circuit of a second nanostructure. As a result we observe a dc current generated in the unbiased second nanostructure. The results can be qualitatively explained in terms of acoustic phonon based energy transfer between the two mutually isolated circuits.

  9. New Spectral Method for Halo Particle Definition in Intense Mis-matched Beams

    An advanced spectral analysis of a mis-matched charged particle beam propagating through a periodic focusing transport lattice is utilized in particle-in-cell (PIC) simulations. It is found that the betatron frequency distribution function of a mismatched space-charge-dominated beam has a bump-on-tail structure attributed to the beam halo particles. Based on this observation, a new spectral method for halo particle definition is proposed that provides the opportunity to carry out a quantitative analysis of halo particle production by a beam mismatch. In addition, it is shown that the spectral analysis of the mismatch relaxation process provides important insights into the emittance growth attributed to the halo formation and the core relaxation processes. Finally, the spectral method is applied to the problem of space-charge transport limits.

  10. Analysis of the injection-locked magnetron with a mismatched circulator

    Based on the scatter matrix of the four-port lossless mismatched circulator, the phase differential equation of the injection-locked magnetron is derived by comparing different effects of the mismatched and perfect circulator on the injection ratio. Besides, the locking range of the injection-locked magnetron with the mismatched circulator is deduced by functional operation. In addition, the phase differential equation and the locked bandwidth of the injection-locked system with a mismatched circulator are compared with those of the small injection-ratio case with a perfect circulator. The influence of the circulator reflection coefficient on the injection-locked magnetron is also analyzed by numerical calculation. Theoretical analysis shows that the decrement of the locked bandwidth is less than 1% and decrement of the stable phase difference is less than 1.2% when the reflection coefficient is less than 0.1. (interdisciplinary physics and related areas of science and technology)

  11. Velocity synchronization of multi-agent systems with mismatched parameters via sampled position data

    Sun, Wen; Huang, Chunli; Lü, Jinhu; Li, Xiong; Chen, Shihua


    Power systems are special multi-agent systems with nonlinear coupling function and symmetric structures. This paper extends these systems to a class of multi-agent systems with mismatched parameters, linear coupling function, and asymmetric structures and investigates their velocity synchronization via sampled position data. The dynamics of the agents is adopted as that of generators with mismatched parameters, while the system structures are supposed to be complex. Two distributed linear consensus protocols are designed, respectively, for multi-agent systems without or with communication delay. Necessary and sufficient conditions based on the sampling period, the mismatched parameters, the delay, and the nonzero eigenvalues of the Laplacian matrix are established. It is shown that velocity synchronization of multi-agent systems with mismatched parameters can be achieved if the sampled period is chosen appropriately. Simulations are given to illustrate the effectiveness of the theoretical results.

  12. Calibration of P/S amplitude ratios for seismic events in Xinjiang and its adjacent areas based on a Bayesian Kriging method

    PAN Chang-zhou; JIN Ping; XIAO Wei-guo


    Correction maps of P/S amplitude ratios for seismic events distributed in Xinjiang, China and its adjacent areas were established using a Bayesian Kriging method for the two seismic stations WMQ and MAK. The relationship between correction maps and variations of along-path features was analyzed and the validity of applying the correction maps to improve performances of P/S discriminants for seismic discrimination was investigated. Results show that obtained correction maps can generally reflect event-station path effects upon corresponding P/S discriminants; and the correction of these effects could further reduce scatters of distance-corrected P/S measurements within earthquake and explosion populations as well as improve their discriminating performances if path effects are a significant factor of such scatters. For example, as corresponding Kriging correction map was applied, the misidentification rate of earthquakes by Pn(2~4 Hz)/Lg(2~4 Hz) at MAK was reduced from 16.3% to 5.2%.

  13. Circuit mismatch influence on performance of paralleling silicon carbide MOSFETs

    Li, Helong; Munk-Nielsen, Stig; Pham, Cam;


    This paper focuses on circuit mismatch influence on performance of paralleling SiC MOSFETs. Power circuit mismatch and gate driver mismatch influences are analyzed in detail. Simulation and experiment results show the influence of circuit mismatch and verify the analysis. This paper aims to give...... suggestions on paralleling discrete SiC MOSFETs and designing layout of power modules with paralleled SiC MOSFETs dies....

  14. Dynamics of beam halo in mismatched beams

    High-power proton linacs for nuclear materials transmutation and production, and new accelerator-driven neutron spallation sources must be designed to control beam-halo formation, which leads to beam loss. The study of particle-core models is leading to a better understanding of the causes and characteristics of beam halo produced by space-charge forces in rms mismatched beams. Detailed studies of the models have resulted in predictions of the dependence of the maximum amplitude of halo particles on a mismatch parameter and on the space-charge tune-depression ratio. Scaling formulas have been derived which will provide guidance for choosing the aperture radius to contain the halo without loss. (author)

  15. Education, Social Mobility, and Talent Mismatch

    Yuki Uchida


    This study presents a two-class, overlapping-generation model featuring social mobility inhibited by the mismatch of talents. Mobility decreases as the private education gap between the two classes widens, whereas it increases with an increased public education spending. Within this framework, we consider the redistributive politics of public education and show that the private education gap provides the government with an incentive to increase public education. We also show that social mobil...

  16. Social Networks and the Labour Market Mismatch

    Kalfa, Eleni; Piracha, Matloob


    This paper assesses the extent to which social contacts and ethnic concentration affect the education-occupation mismatch of natives and immigrants. Using Australian panel data and employing a dynamic random effects probit model, we show that social capital exacerbates the incidence of over-education, particularly for females. Furthermore, for the foreign-born, ethnic concentration significantly increases the incidence of over-education. Using an alternative index, we also show that social pa...

  17. 基于邻接矩阵的软件测试环境规划设计方法%Design Method of Software Test Environment Based on Adjacency Matrix

    张晓娜; 金朋飞; 严长伟


    在分析软件测试环境的基础上,研究了软件试验中测试用例设计面临的突出问题,从软件测试环境的规划出发,基于有向图的邻接矩阵,设计满足一定覆盖度标准的软件测试环境规划方法,可为软件试验提供技术参考和研究方向。%Based on the analysis about the software test environment, this paper studies the key problems of the test case de⁃sign in the software test, based on the adjacency matrix of directed graph, designs the software test environment plan method up to coverage criteria. All these researches can provide technology reference for the future development of software test.

  18. Ecologic and geographic distributions of the vascular plants of southern Nye County, and adjacent parts of Clark, Lincoln, and Esmeralda Counties, Nevada. [Based on collections made in 1970

    Beatley, J. C.


    A catalog is compiled of the vascular plants indiginous to Nye, Clark, Lincoln, and Esmeralda Counties of Nevada based on collections made in 1970. This compilation is an update of previous collections in these areas and is a supplement to report, UCLA--12-705. (ERB)

  19. Kinetics and binding of the thymine-DNA mismatch glycosylase, Mig-Mth, with mismatch-containing DNA substrates.

    Begley, Thomas J; Haas, Brian J; Morales, Juan C; Kool, Eric T; Cunningham, Richard P


    We have examined the removal of thymine residues from T-G mismatches in DNA by the thymine-DNA mismatch glycosylase from Methanobacterium thermoautrophicum (Mig-Mth), within the context of the base excision repair (BER) pathway, to investigate why this glycosylase has such low activity in vitro. Using single-turnover kinetics and steady-state kinetics, we calculated the catalytic and product dissociation rate constants for Mig-Mth, and determined that Mig-Mth is inhibited by product apyrimidinic (AP) sites in DNA. Electrophoretic mobility shift assays (EMSA) provide evidence that the specificity of product binding is dependent upon the base opposite the AP site. The binding of Mig-Mth to DNA containing the non-cleavable substrate analogue difluorotoluene (F) was also analyzed to determine the effect of the opposite base on Mig-Mth binding specificity for substrate-like duplex DNA. The results of these experiments support the idea that opposite strand interactions play roles in determining substrate specificity. Endonuclease IV, which cleaves AP sites in the next step of the BER pathway, was used to analyze the effect of product removal on the overall rate of thymine hydrolysis by Mig-Mth. Our results support the hypothesis that endonuclease IV increases the apparent activity of Mig-Mth significantly under steady-state conditions by preventing reassociation of enzyme to product. PMID:12509271

  20. Methodical approaches to the implementation of radiation-ecological and sanitary investigation of the areas of the former military bases and recreation zones adjacent to them

    According to the Decree of the Council of Ministers of the Republic of Belarus N856 dated 21.12.93, the possibility of using the former military bases for remediation of the population from the Chernobyl accident affected areas has been investigated. It was necessary to conduct a detailed investigation of the territory, as there was no information on sanitary and radioecological state on the sites. Firstly, contamination level and contamination areals were to be defined, as well as evaluation of danger risk for the population. Secondly, the obtained results of the investigation should form the basis for further estimation of the environment state on the sites, as well as for decision making on the territory remediation

  1. 基于粗糙集的相邻交叉口信号控制方法研究%Adjacent Intersection Signal Control Based on Rough Set

    李璐兵; 陈荣升


    复杂交通区域的交通状况难以利用具体模型直接描述,而粗糙集理论能够在分析大量经验数据的基础上找到用于决策的规则。以交大东路上的行人和机动车为研究对象,提出一种基于粗糙集的信号配时算法,对交大东路上的两个相邻交叉口的信号灯进行控制,提出控制规则,对该复杂交通区域进行优化和疏导,是对多交叉口交通管理与控制的一次有益尝试。%It′s difficult to accurately describe the traffic condition in complex traffic areas using specific formulas. But by analyzing plenty of data based on rough set theory, rules can be found for decision-mak-ing. The article put pedestrians and vehicles on Jiaoda-East Road as the research object, and then pre-sented with a signal algorithm based on rough set to control two neighboring traffic lights in the road and reduce traffic pressure in this complex traffic area. Finally, it proposed control rules and guidance to opti-mize the complex traffic area, which was a beneficial trial for multiple intersections group on traffic man-agement and control.

  2. Visual mismatch negativity: A predictive coding view

    Gabor eStefanics


    Full Text Available An increasing number of studies investigate the visual mismatch negativity (vMMN or use the vMMN as a tool to probe various aspects of human cognition. This paper reviews the theoretical underpinnings of vMMN in the light of methodological considerations and provides recommendations for measuring and interpreting the vMMN. The following key issues are discussed from the experimentalist’s point of view in a predictive coding framework: 1 experimental protocols and procedures to control ‘refractoriness’ effects; 2 methods to control attention; 3 vMMN and veridical perception.

  3. Barbed Congruence of Asymmetry and Mismatch

    Xiao-Ju Dong; Yu-Xi Fu


    The X calculus is a model of concurrent and mobile systems. It emphasizes that communications are information exchanges. In the paper, two constructions are incorporated into the framework of the chi calculus, which are asymmetric communication and mismatch condition widely used in applications. Since the barbed bisimilarity has proved its generality and gained its popularity as an effective approach to generating a reasonable observational equivalence, we study both the operational and algebraic properties of the barbed bisimilarity in this enriched calculus. The investigation supports an improved understanding of the bisimulation behaviors of the model. It also gives a general picture of how the two constructions affect the observational theory.

  4. Kauffman's adjacent possible in word order evolution

    Ferrer-i-Cancho, Ramon


    Word order evolution has been hypothesized to be constrained by a word order permutation ring: transitions involving orders that are closer in the permutation ring are more likely. The hypothesis can be seen as a particular case of Kauffman's adjacent possible in word order evolution. Here we consider the problem of the association of the six possible orders of S, V and O to yield a couple of primary alternating orders as a window to word order evolution. We evaluate the suitability of various competing hypotheses to predict one member of the couple from the other with the help of information theoretic model selection. Our ensemble of models includes a six-way model that is based on the word order permutation ring (Kauffman's adjacent possible) and another model based on the dual two-way of standard typology, that reduces word order to basic orders preferences (e.g., a preference for SV over VS and another for SO over OS). Our analysis indicates that the permutation ring yields the best model when favoring pa...

  5. What Do Educational Mismatches Tell Us About Skill Mismatches? A Cross-country Analysis

    Allen, Jim; Weert, de Egbert


    The relationship between higher education and employment is commonly interpreted in terms of the extent to which the higher education sector is providing graduates with the knowledge and skills to match employment needs. It is assumed that a mismatch between working in a job and level or field of ed

  6. How relevant is job mismatch for German graduates?

    Berlingieri, Francesco; Erdsiek, Daniel


    In this study, we examine the incidence and direct consequences of job mismatch for German graduates. Beyond measuring job mismatch by the comparison of qualification obtained by employees and required for a job, we employ self-reported skill mismatch variables concerning overall skills and more detailed information about the surplus or deficit in specific competences. The results indicate that a substantial share of graduates underutilizes own skills in the job. The rate of overqualification...

  7. A Model Driven approach for Applicationspecific Mismatch Detection

    Amit Raj


    Several large-scale systems are developed in modules that are assembled together to deliver a desired functionality. The modules developed in isolation may not implement the desired requirements. Itcreates several problems either at design-time, deployment-time or at run-time. Existing research defines a class of such problems as mismatches. A mismatch may cause malfunctioning or crash of a system.Therefore, a mismatch needs to be detected and resolved. The paper describes a model-driven gene...

  8. Quantum noise in parametric amplification under phase-mismatched conditions

    Inoue, K.


    This paper studies quantum noise in parametric amplification under phase-mismatched conditions. The equations of motion of the quantum-mechanical field operators, which include phase mismatch under unsaturated conditions are first derived from the Heisenberg equation. Next, the noise figure is evaluated using the solutions of the derived equations. The results indicate that phase mismatch scarcely affects noise property in phase-insensitive amplification while it has a notable effect in case of phase-sensitive amplification.

  9. An IQ mismatch calibration and compensation technique for wideband wireless transceivers

    Jin, Peng; Liguo, Zhou; Heng, Yao; Fang, Yuan; Zhi, Fang; Yin, Shi


    An IQ mismatch calibration and compensation technique based on the digital baseband for wideband wireless communication transmitters is proposed. The digital baseband transmits the signal used for IQ mismatch calibration. The signal passes through the RF transmitter path, the calibration loop (which is composed of a square power detector and a band-pass filter in the RF transceiver) and the variable gain amplifier of the receiver. The digital baseband samples the signal for IQ mismatch estimation and compensates for it. Compared with the self-calibration technique in the RF chip, the proposed technique saves area and power consumption for the wireless local area network solution. This technique has been successfully used for the 802.11n system and satisfies the requirement of the standard by achieving over 50 dB image suppression.

  10. An IQ mismatch calibration and compensation technique for wideband wireless transceivers

    An IQ mismatch calibration and compensation technique based on the digital baseband for wideband wireless communication transmitters is proposed. The digital baseband transmits the signal used for IQ mismatch calibration. The signal passes through the RF transmitter path, the calibration loop (which is composed of a square power detector and a band-pass filter in the RF transceiver) and the variable gain amplifier of the receiver. The digital baseband samples the signal for IQ mismatch estimation and compensates for it. Compared with the self-calibration technique in the RF chip, the proposed technique saves area and power consumption for the wireless local area network solution. This technique has been successfully used for the 802.11n system and satisfies the requirement of the standard by achieving over 50 dB image suppression. (semiconductor integrated circuits)

  11. Labour Market Mismatch among UK Graduates: An Analysis Using REFLEX Data

    McGuinness, Seamus; Sloane, Peter J.


    There is much disagreement in the literature over the extent to which graduates are mismatched in the labour market and the reasons for this. In this paper we utilise the Flexible Professional in the Knowledge Society (REFLEX) data set to cast light on these issues, based on data for UK graduates. We find substantial pay penalties for…

  12. The mouse mismatch repair protein, MSH3, is a nucleoplasmic protein that aggregates into denser nuclear bodies under conditions of stress.

    Holt, I.; Thanh Lam, L.; Tome, S.; Wansink, D.G.; Riele, H. te; Gourdon, G.; Morris, G.E.


    The mismatch repair protein, MSH3, together with MSH2, forms the MutSbeta heterodimer which recognizes and repairs base pair mismatches and larger insertion/deletion loops in DNA. Lack of specific antibodies against mouse MSH3 has hampered studies of its expression and localization. Mouse MSH3 is no

  13. Local approach to strength mis-match effect on cleavage fracture of notched material

    This paper discusses the strength mis-match effect on cleavage fracture of notched materials based on the local approach. The aim of the study is to verify the independence of the critical Weibull stress at fracture on the strength mis-match condition. Diffusion bonded joints were made with two materials S and H different in strength level. Two types of 3-point bend specimen were extracted. One had a notch in the lower strength material S near the bonded interface, and another in the higher strength material H. Homogeneous specimens were also made for each material. The critical CTOD value at cleavage fracture for the overmatched specimen with a notch in the material S was smaller than that of the homogeneous specimen of the material S. By contrast, the undermatched specimen with a notch in the material H showed apparently larger critical CTOD value than the homogeneous specimen of the material H. The strength mis-match effect on the CTOD results was explained in terms of the constraint effect on the near-tip stress field. Namely, the near-tip stress is elevated by strength overmatching and relaxed by strength undermatching. The Weibull stresses for the homogeneous and mis-matched specimens were evaluated in the light of the near-tip stress fields. The critical Weibull stress at fracture did not depend on the strength mis-match condition near the notch. By using the critical Weibull stress obtained for the homogeneous specimen, the strength mis-match effect on the CTOD results can be predicted. Close agreement was observed between the critical CTOD value predicted and that obtained by the experiment. (orig.)

  14. Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene

    Mutations affecting heteroduplex DNA mismatch repair in Streptococcus pneumoniae were localized in two genes, hexA and hexB, by fractionation of restriction fragments carrying mutant alleles. A fragment containing the hexA4 allele was cloned in the S. pneumoniae cloning system, and the hexA+ allele was introduced into the recombinant plasmid by chromosomal facilitation of plasmid transfer. Subcloning localized the functional hexA gene to a 3.5-kilobase segment of the cloned pneumococcal DNA. The product of this gene was shown in Bacillus subtilis minicells to be a polypeptide with an M/sub r/ of 86,000. Two mutant alleles of hexA showed partial expression of the repair system when present in multicopy plasmids. A model for mismatch repair, which depends on the interaction of two protein components to recognize the mismatched base pair and excise a segment of DNA between strand breaks surrounding the mismatch, is proposed

  15. Supply-demand mismatch transients in susceptible peri-infarct hot zones explain the origin of spreading injury depolarizations

    von Bornstädt, Daniel; Houben, Thijs; Seidel, Jessica; Zheng, Yi; Dilekoz, Ergin; Qin, Tao; Sandow, Nora; Kura, Sreekanth; Eikermann-Haerter, Katharina; Endres, Matthias; Boas, David A.; Moskowitz, Michael A.; Lo, Eng H.; Dreier, Jens P.; Woitzik, Johannes; Sakadžić, Sava; Ayata, Cenk


    SUMMARY Peri-infarct depolarizations (PIDs) are seemingly spontaneous spreading depression-like waves that negatively impact tissue outcome in both experimental and human stroke. Factors triggering PIDs are unknown. Here, we show that somatosensory activation of peri-infarct cortex triggers PIDs when the activated cortex is within a critical range of ischemia. We show that the mechanism involves increased oxygen utilization within the activated cortex, worsening the supply-demand mismatch. We support the concept by clinical data showing that mismatch predisposes to PIDs in human stroke as well. Conversely, transient worsening of mismatch by episodic hypoxemia or hypotension also reproducibly triggers PIDs. Therefore, PIDs are triggered upon supply-demand mismatch transients in metastable peri-infarct hot zones due to increased demand or reduced supply. Based on the data, we propose that minimizing sensory stimulation and hypoxic or hypotensive transients in stroke and brain injury would reduce PID incidence and their adverse impact on outcome. PMID:25741731

  16. Skill Mismatch of Graduates in a Local Labour Market

    Enrico Marelli


    Full Text Available In this paper we first review the (potential and actual role of the Universities for the local economies in which they operate, especially considering the implications deriving from the degree of skill mismatch (over-education in a local labour market. Then, in the second part of the paper, we realise an empirical investigation based on administrative information of an Italian University matched with the data of the job centres of the local (provincial labour market in order to reconstruct the characteristics of the university-to-work transitions of graduates. Our results have important policy implications, since for local development it is crucial, among other things, to make the best use of all human resources and especially those with the highest educational level.

  17. Mismatch-Shaping Serial Digital-to-Analog Converter

    Steensgaard-Madsen, Jesper; Moon, Un-Ku; Temes, Gabor C.


    A simple but accurate pseudo-passive mismatch-shaping D/A converter is described. A digital state machine is used to control the switching sequence of a symmetric two-capacitor network that performs the D/A conversion. The error caused by capacitor mismatch is uncorrelated with the input signal a...

  18. Mismatch-Shaped Pseudo-Passive Two-Capacitor DAC

    Steensgaard-Madsen, Jesper; Moon, Un-Ku; Temes, Gabor C.


    A simple mismatch-shaping scheme is proposed for a two-capacitor DAC. Unlike in other mismatch-shaping systems, the shaped error is generated by direct filtering of a well-defined bounded signal, which can be generated as white noise. The operation is closely related to a specific digital interpo...

  19. The mismatch between job openings and job seekers

    Canon, Maria E.; Mingyu Chen


    Today's high unemployment rate is often linked to a structural imbalance—a mismatch between the skills and location required to fill vacant jobs and the skills and geographical preferences of the unemployed. But the evidence downplays the role of this mismatch.

  20. Improved DNA clamps by stacking to adjacent nucleobases

    Fatthalla, M.I.; Pedersen, Erik Bjerregaard


    Three or four aromatic rings interconnected by acetylene bridges form a stiff conjugated system with sufficient conformational freedom to make it useful to link together the two strands of a DNA clamp. Upon targeting a ssDNA, the conformational flexibility allows better stacking of the linker to...... the underlying non-planar base triplet in the formed triplex. This type of triplexes has a substantially higher thermal melting temperature which can be further improved by inserting locked nucleic acids (LNAs) in the Hoogsteen part of the clamp. An extremely high sensitivity to mismatches is observed...

  1. Reducing measurement scale mismatch to improve surface energy flux estimation

    Iwema, Joost; Rosolem, Rafael; Rahman, Mostaquimur; Blyth, Eleanor; Wagener, Thorsten


    Soil moisture importantly controls land surface processes such as energy and water partitioning. A good understanding of these controls is needed especially when recognizing the challenges in providing accurate hyper-resolution hydrometeorological simulations at sub-kilometre scales. Soil moisture controlling factors can, however, differ at distinct scales. In addition, some parameters in land surface models are still often prescribed based on observations obtained at another scale not necessarily employed by such models (e.g., soil properties obtained from lab samples used in regional simulations). To minimize such effects, parameters can be constrained with local data from Eddy-Covariance (EC) towers (i.e., latent and sensible heat fluxes) and Point Scale (PS) soil moisture observations (e.g., TDR). However, measurement scales represented by EC and PS still differ substantially. Here we use the fact that Cosmic-Ray Neutron Sensors (CRNS) estimate soil moisture at horizontal footprint similar to that of EC fluxes to help answer the following question: Does reduced observation scale mismatch yield better soil moisture - surface fluxes representation in land surface models? To answer this question we analysed soil moisture and surface fluxes measurements from twelve COSMOS-Ameriflux sites in the USA characterized by distinct climate, soils and vegetation types. We calibrated model parameters of the Joint UK Land Environment Simulator (JULES) against PS and CRNS soil moisture data, respectively. We analysed the improvement in soil moisture estimation compared to uncalibrated model simulations and then evaluated the degree of improvement in surface fluxes before and after calibration experiments. Preliminary results suggest that a more accurate representation of soil moisture dynamics is achieved when calibrating against observed soil moisture and further improvement obtained with CRNS relative to PS. However, our results also suggest that a more accurate

  2. Investigation on the influence of BBO thermal-induced phase mismatching in 266nm UV laser conversion efficiency

    Yu, Kai; Jin, Guangyong; Yu, Miao; Huang, Zhulong; Zhai, Ruizhi; Wang, Lei


    266nm UV laser has a wide range of applications in many fields, such as laser medical treatment, laser processing, precision measure and other applications for the reason of its advantages in wavelength, small diffraction effects, high single-photon energy, and high resolution and so on. BBO crystals absorb parts of the fundamental laser energy and second harmonic laser energy are unavoidable, and thus the temperature raise, so that the existing crystal phase matching conditions change, resulting in phase mismatching in the high-power frequency doubling, greatly influence the 266nm UV laser conversion efficiency. To further study the effect of phase mismatching to conversion efficiency, and improve the conversion efficiency, output power and other output characteristics of 266nm laser, the article mainly describe from the following three aspects. Firstly, took the use of three-dimensional nonlinear crystal temperature distribution which is obtained, the process of BBO crystal thermal-induced phase mismatching is analyzed. Secondly, based on frequency doubling theory, the effects of the thermal-induced phase mismatching affected of conversion in crystals are analyzed. Combining with the phase mismatching of the three-dimensional distributions, the fourth harmonic conversion efficiency with thermal phase mismatching changes of BBO 266nm UV laser are simulated for the first time. Thirdly, by using MATLAB software, the effects of phase mismatching to conversion efficiency in crystal for different waist radius, 532nm laser power and the fundamental beam quality are simulated. The results indicate a good physical interpretation of reasons of high power laser frequency doubling system. It shows that the model established explains the reason of the reduction of conversion efficiency, output power and the beam quality excellently. All results make a leading sense to the research on the compensating of the phase mismatching and on the improvement of conversion efficiency.

  3. Mismatching Perspectives and Pacific Transculturality”

    Anne Holden Rønning


    Full Text Available Increased critical consciousness and awareness of interculturality in a global and glocal context at the beginning of the twenty - first century has increasingly used the concept of transculturation when discussing modernities. Politically transculturation can be used to describe processes of negotiation in contemporary society that lead to social awareness and solidarity, as well as ensuring the continuity of societies. The fusing of cultural forms leads to a mismatching of perspectives, hence some critics have preferred to use the terms translation and/or transliteracy to describe this concept. Transculturation is related to the “normal processes of artistic borrowing and influence, by which any cul ture makes part of its contribution to the conversation of mankind,” as Les Murray maintained, and “it engages multiple lines of difference simultaneous ly” with overlapping boundaries (Rogers 491. Referring to various authors and linking it to cultural ap propriation and border crossings, this article examines how the narrative expression of Both Sides of the Moon, to cite the title of Alan Duff’s book, is a key feature of Pacific writing, in an area where centuries of migration from near and far have expos ed different cultures to each other on social, political, linguistic and aesthetic levels. These ‘contact zones’, to use Mary Pratt’s words, provide the reader with constantly moving translated identities, cultural hybridity and a use of language that has a highly local significance in a global context.

  4. Novel Rx IQ mismatch compensation considering laser phase noise for CO-OFDM system

    Ma, Xiurong; Ding, Zhaocai; Li, Kun; Wang, Xiao


    In this paper, a novel compensation scheme for receiver (Rx) in-phase/quadrature (IQ) mismatch is proposed in coherent optical orthogonal frequency division multiplexing (CO-OFDM) system in the presence of laser phase noise. In this scheme, laser phase noise and channel distortion were combined as a new channel transfer factor, the IQ mismatch factor and initial channel transfer factor can be estimated independently based on the relationship of IQ mismatch factors. And the channel transfer factor can be updated on a symbol-by-symbol basis which retrieves an estimation of the phase noise value by extracting and averaging the phase drift of all OFDM sub-channels. Numerical results indicate that when the phase and amplitude mismatch are 10° and 2 dB respectively, a 1.6 dB optical signal-to noise ratio is improved at laser linewidth of 60 kHz. Furthermore, the complexity of the proposed method is analyzed in terms of the number of required complex multiplications per bit.

  5. Effects of GA mismatches on the structure and thermodynamics of RNA internal loops

    UV melting, CD and NMR studies indicate rGCGAGCG and rGCAGGCG from unusually stable duplexes of type a and b. The observed ΔG degree 37's in 1 M NaCl are -6.7 and -6.3 kcal/mol, respectively. For the related duplex, c,ΔG degree 37 is -4.2kcal/mol. The predicted ΔG degree 37 from nearest-neighbor parameters for all three duplexes is -4.7 kcal/mol. The results suggest a special interaction in the duplexes containing GA mismatches. Presumably, this is hydrogen bonding between G and A. While the thermodynamics for (rGCGAGCG)2 and (rGCAGGCG)2 are similar, CD and the imino region of the proton NMR spectra indicate their structures are different. The NMR spectrum of (rGCAGGCG)2 has a resonance corresponding to a hydrogen-bonded GA mismatch, while for (rGCGAGCG)2 no hydrogen-bonded imino proton is observed for the mismatch. The glycosidic torsion angles of the bases in the GA mismatches of (rGCAGGCG)2 and (rCGCAGGCG)2 are anti. Duplexes of type d, where X is A, G, or U, are more stable than e, and the stability differences are similar to those observed for f versus g. Thus, 3'-dangling ends in this system make contributions to duplex stability that are similar to contributions observed with fully paired duplexes

  6. Functional distance between recipient and donor HLA-DPB1 determines nonpermissive mismatches in unrelated HCT.

    Crivello, Pietro; Heinold, Andreas; Rebmann, Vera; Ottinger, Hellmut D; Horn, Peter A; Beelen, Dietrich W; Fleischhauer, Katharina


    The role of HLA amino acid (AA) polymorphism for the outcome of hematopoietic cell transplantation (HCT) is controversial, in particular for HLA class II. Here, we investigated this question in nonpermissive HLA-DPB1 T-cell epitope (TCE) mismatches reflected by numerical functional distance (FD) scores, assignable to all HLA-DPB1 alleles based on the combined impact of 12 polymorphic AAs. We calculated the difference in FD scores (ΔFD) of mismatched HLA-DPB1 alleles in patients and their 10/10 HLA-matched unrelated donors of 379 HCTs performed at our center for acute leukemia or myelodysplastic syndrome. Receiver-operator curve-based stratification into 2 ΔFD subgroups showed a significantly higher percentage of nonpermissive TCE mismatches for ΔFD >2.665, compared with ΔFD ≤2.665 (88% vs 25%, P 2.665 was significantly associated with overall survival (hazard ratio [HR], 1.40; 95% confidence interval [CI], 1.05-1.87; P TCE mismatches. There was a marked but not statistically significant increase in the hazards of relapse and nonrelapse mortality in the high ΔFD subgroup, whereas no differences were observed for acute and chronic graft-versus-host disease. Seven nonconservative AA substitutions in peptide-binding positions had a significantly stronger impact on ΔFD compared with 5 others (P = .0025), demonstrating qualitative differences in the relative impact of AA polymorphism in HLA-DPB1. The novel concept of ΔFD sheds new light onto nonpermissive HLA-DPB1 mismatches in unrelated HCT. PMID:27162243

  7. Test Mismatch in Switched-Current Circuits Using Wavelet Analysis"

    GUO Jierong; HE Yigang; LIU Meirong; TANG Shengxue; LI Hongmin


    Errors of mismatch and currents calibration caused by channel geometrical variety in switched-current are investigated in this paper. The relation and computing of mismatch and sensitivity are discussed also, and then a measure method of switched current mismatch using wavelet decomposition is proposed. A selected group of same transconductance is choosing as a cohort firstly, and the sensitivities of cohort in relation to the variation of transconductance are computed. Compared with the nominal deviation and tolerance borderline, the optimization and testing can be performed. As an example, a sixth order chebyshev low-pass filter is simulated and tested. The results have justified the reliability and feasibility of the method.

  8. Homogenizing the dose for adjacent fields

    Difficulties are found in radiotherapy in the determination of the gap which should be left among adjacent fields on the skin. In order to homogenize the dose at a given depth, measurements are done with a wood phantom using films, thermoluminescent dosemeters and ionization chambers. Field match is checked according to tables related in the literature and experimental data. Two tables of field separation are built at various depths, one for simultaneous adjacent fields and the other for non-simultaneous adjacent fields. Tables must be checked and the additional distances corrected for each field

  9. Mismatch oligonucleotides in human and yeast: guidelines for probe design on tiling microarrays

    Jee Justin


    Full Text Available Abstract Background Mismatched oligonucleotides are widely used on microarrays to differentiate specific from nonspecific hybridization. While many experiments rely on such oligos, the hybridization behavior of various degrees of mismatch (MM structure has not been extensively studied. Here, we present the results of two large-scale microarray experiments on S. cerevisiae and H. sapiens genomic DNA, to explore MM oligonucleotide behavior with real sample mixtures under tiling-array conditions. Results We examined all possible nucleotide substitutions at the central position of 36-nucleotide probes, and found that nonspecific binding by MM oligos depends upon the individual nucleotide substitutions they incorporate: C→A, C→G and T→A (yielding purine-purine mispairs are most disruptive, whereas A→X were least disruptive. We also quantify a marked GC skew effect: substitutions raising probe GC content exhibit higher intensity (and vice versa. This skew is small in highly-expressed regions (± 0.5% of total intensity range and large (± 2% or more elsewhere. Multiple mismatches per oligo are largely additive in effect: each MM added in a distributed fashion causes an additional 21% intensity drop relative to PM, three-fold more disruptive than adding adjacent mispairs (7% drop per MM. Conclusion We investigate several parameters for oligonucleotide design, including the effects of each central nucleotide substitution on array signal intensity and of multiple MM per oligo. To avoid GC skew, individual substitutions should not alter probe GC content. RNA sample mixture complexity may increase the amount of nonspecific hybridization, magnify GC skew and boost the intensity of MM oligos at all levels.

  10. L-band AlGaN/GaN Power Amplifier with Protection Against Load Mismatch

    Heijningen , M. van; Bent, G. van der; Houwen, E.H. van de; A Chowdhary; Vliet, F.E. van


    Solid-state power amplifiers need protection at the output to handle high reflections due to mismatch. Normally this is implemented by using a ferrite-based isolator. These are however large and bulky components. This paper presents a Gallium-Nitride power amplifier module with automatic protection against large reflections based on fold-back protection, by sensing the reflected power. Measurements have been performed on a 100 W L-band power amplifier module at full reflection (short at the o...

  11. Detect Adjacent Well by Analyzing Geomagnetic Anomalies

    Su Zhang


    Full Text Available This study describes a method of determining the position of adjacent well by analyzing geomagnetic anomalies in the drilling. In the experiment, put a casing in the geomagnetic field respectively to simulate 3 conditions, which are vertical well, deviated well and horizontal well. Study the interference of regional geomagnetic caused by casing, summary the law of the regional geomagnetic field anomalies caused by the adjacent casing. Experimental results show that: magnetic intensity distortion caused by deviated well is similar to that caused by horizontal well, but the distortion is different from vertical well. The scope and amplitude of N and E component magnetic intensity distortion will increase with the increase of casing inclination, meanwhile the scope and amplitude of V component distortion will decrease and the distortion value changes from negative to positive to the southwest of adjacent well. Through the analysis of geomagnetic anomalies, the position of the adjacent wells could be determined.

  12. 基于生态修复的青海湖沙柳河鱼道建设与维护%Construction and management of fish passage on Shaliu River adjacent to Qinghai Lake based on ecological restoration

    吴晓春; 史建全


    Gymnocypris przewalskii belongs to subfamily of Schizothoracinae, family of Cyprinidae and order of Cypriniformes, and it is an endemic species only distributed in the Qinghai Lake and its adjacent rivers and lakes. It possesses migration characteristics, and goes upstream from the lake to surrounding rivers in the reproduction season. However, several dams built on the rivers flowing into the lake with the agricultural development in the Qinghai Lake region since 1970s, severely blocked its migration passages, leading to obviously decreasing of resources of Gymnocypris przewalskii in recent years. To protect Gymnocypris przewalskii, three kinds of fish passages on the dams of rivers flowing into the Qinghai Lake were introduced, which were types of guide wall, simple steel modular and ladder slope, respectively. 1) The guide wall type fish passage was separately constructed on the dams in the Shaliu River, Quanji River and Haergai River in 2006, which were built along river side, but it had several defects because of the lack of experience. 2) The simple steel modular type fish passage was designed and constructed on the dam of the Shaliu River in 2008, Based on biological characteristics of Gymnocypris przewalskii and the basic situation of the dam and environmental conditions of the Shaliu River. After considering inlet location of fish passage, suction flow and hydraulic conditions within passage, the inlet was located at the area with aggregated fish and slow current velocity. The water flow was 0.5 m/s, the current velocity was 0.1-0.25 m3/s, as well as the slope of 1:10 within fish passage. 3) The ladder slope type fish passage was built on the dam of Shaliu River in 2010, which used two types open fishponds of multi-ladder and slight slope. Fish passage without hindrance had been almost come to be real through this type. This type was designed according to basic theories and related parameters of simple fish passage. The length was 80 m and the width was 1

  13. Adjacency Algebra of Unitary Cayley Graph

    A. Satyanarayana Reddy


    Full Text Available A few properties of unitary Cayley graphs are explored using their eigenvalues. It is shown that the adjacency algebra of a unitary Cayley graph is a coherent algebra. Finally, a class of unitary Cayley graphs that are distance regular are also obtained.Key Words: Adjacency Algebra, Circulant Graph, Coherent Algebra, Distance Regular Graph,Ramanujan's sum .AMS(2010: 05C25, 05C50

  14. Protein-protein interactions in DNA mismatch repair.

    Friedhoff, Peter; Li, Pingping; Gotthardt, Julia


    The principal DNA mismatch repair proteins MutS and MutL are versatile enzymes that couple DNA mismatch or damage recognition to other cellular processes. Besides interaction with their DNA substrates this involves transient interactions with other proteins which is triggered by the DNA mismatch or damage and controlled by conformational changes. Both MutS and MutL proteins have ATPase activity, which adds another level to control their activity and interactions with DNA substrates and other proteins. Here we focus on the protein-protein interactions, protein interaction sites and the different levels of structural knowledge about the protein complexes formed with MutS and MutL during the mismatch repair reaction. PMID:26725162

  15. Understanding the Consequences of Property Rights Mismatches: a Case Study of New Zealand's Marine Resources

    Tracy Yandle


    Full Text Available Within fisheries and natural resource management literature, there is considerable discussion about the key roles that property rights can play in building biologically and socially sustainable resource management regimes. A key point of agreement is that secure long-term property rights provide an incentive for resource users to manage the resource sustainably. However, property rights mismatches create ambiguity and conflict in resource use. Though the term mismatches is usually associated with problems in matching temporal and spatial resource characteristics with institutional characteristics, I expand it here to include problems that can arise when property rights are incompletely defined or incompletely distributed. Property rights mismatches are particularly likely to occur over marine resources, for which multiple types of resource and resource user can be engaged and managed under a variety of regulatory regimes. I used New Zealand's marine resources to examine the causes and consequences of these property rights mismatches. New Zealand is particularly interesting because its property-rights-based commercial fishing regime, in the form of individual transferable quotas, has attracted considerable positive attention. However, my review of the marine natural resource management regime from a broader property rights perspective highlights a series of problems caused by property rights mismatches, including competition for resources among commercial, customary, and recreational fishers; spatial conflict among many marine resource users; and conflicting incentives and objectives for the management of resources over time. The use of a property rights perspective also highlights some potential solutions such as the layering of institutional arrangements and the improvement of how property rights are defined to encourage long-term sustainability.

  16. Mismatch characteristics of optical parametric chirped pulse amplification

    Novák, Ondřej; Turčičová, Hana; Divoký, Martin; Huynh, Jaroslav; Straka, Petr


    Roč. 11, č. 2 (2014), 1-7. ISSN 1612-2011 R&D Projects: GA ČR GA202/06/0814; GA MŠk(CZ) LC528 Institutional support: RVO:68378271 Keywords : phase matching * phase mismatch * beam mismatch * broadband amplification * parametric amplifiers * OPCPA * iodine laser Subject RIV: BH - Optics , Masers, Laser s Impact factor: 2.458, year: 2014

  17. A Trainable Neuromorphic Integrated Circuit that Exploits Device Mismatch

    Thakur, Chetan Singh; Wang, Runchun; Hamilton, Tara Julia; Tapson, Jonathan; van Schaik, Andre


    Random device mismatch that arises as a result of scaling of the CMOS (complementary metal-oxide semi-conductor) technology into the deep submicron regime degrades the accuracy of analogue circuits. Methods to combat this increase the complexity of design. We have developed a novel neuromorphic system called a Trainable Analogue Block (TAB), which exploits device mismatch as a means for random projections of the input to a higher dimensional space. The TAB framework is inspired by the princip...

  18. Rhabdomyosarcoma in patients with constitutional mismatch-repair-deficiency syndrome

    Kratz, Christian P; Holter, Spring; Etzler, Julia; Lauten, Melchior; Pollett, Aaron; Charlotte M. Niemeyer; Gallinger, Steven; Wimmer, Katharina


    Abstract Background: Biallelic germline mutations in the mismatch repair genes MLH1, MSH2, MSH6 or PMS2 cause a recessive childhood cancer syndrome characterized by early-onset malignancies and signs reminiscent of neurofibromatosis type 1 (NF1). Alluding to the underlying genetic defect we refer to this syndrome as constitutional mismatch repair-deficiency (CMMR-D) syndrome. The tumor spectrum of CMMR-D syndrome includes hematological neoplasias, brain tumors and Lynch syndrome as...

  19. The effects of nucleotides on MutS-DNA binding kinetics clarify the role of MutS ATPase activity in mismatch repair

    Jacobs-Palmer, Emily; Hingorani, Manju M.


    MutS protein initiates mismatch repair with recognition of a non-Watson-Crick base pair or base insertion/deletion site in DNA, and its interactions with DNA are modulated by ATPase activity. Here we present a kinetic analysis of these interactions, including the effects of ATP binding and hydrolysis, reported directly from the mismatch site by 2-aminopurine fluorescence. When free of nucleotides, the T. aquaticus MutS dimer binds a mismatch rapidly (kON = 3 x 106 M−1s−1) and forms a stable c...

  20. Image edge-enhancement in optical microscopy with a phase mismatched spiral phase plate

    Shibiao Wei; Jing Bu; Siwei Zhu; Xiaocong Yuan


    @@ We present a spiral phase filtering system with a large tolerance for edge enhancement of both phase and amplitude objects in optical microscopy.The method is based on a Fourier 4-f spatial filtering system.A phase mismatched spiral phase plate (SPP) fabricated by electron beam lithography is employed as the radial Hilbert transform for image edge enhancement.Compared with holography, SPP is simple,economical, reliable, and easy to integrate.%We present a spiral phase filtering system with a large tolerance for edge enhancement of both phase and amplitude objects in optical microscopy. The method is based on a Fourier 4-f spatial filtering system.A phase mismatched spiral phase plate (SPP) fabricated by electron beam lithography is employed as the radial Hilbert transform for image edge enhancement. Compared with holography, SPP is simple,economical, reliable, and easy to integrate.

  1. Molecularly resolved label-free sensing of single nucleobase mismatches by interfacial LNA probes.

    Mishra, Sourav; Lahiri, Hiya; Banerjee, Siddhartha; Mukhopadhyay, Rupa


    So far, there has been no report on molecularly resolved discrimination of single nucleobase mismatches using surface-confined single stranded locked nucleic acid (ssLNA) probes. Herein, it is exemplified using a label-independent force-sensing approach that an optimal coverage of 12-mer ssLNA sensor probes formed onto gold(111) surface allows recognition of ssDNA targets with twice stronger force sensitivity than 12-mer ssDNA sensor probes. The force distributions are reproducible and the molecule-by-molecule force measurements are largely in agreement with ensemble on-surface melting temperature data. Importantly, the molecularly resolved detection is responsive to the presence of single nucleobase mismatches in target sequences. Since the labelling steps can be eliminated from protocol, and each force-based detection event occurs within milliseconds' time scale, the force-sensing assay is potentially capable of rapid detection. The LNA probe performance is indicative of versatility in terms of substrate choice - be it gold (for basic research and array-based applications) or silicon (for 'lab-on-a-chip' type devices). The nucleic acid microarray technologies could therefore be generally benefited by adopting the LNA films, in place of DNA. Since LNA is nuclease-resistant, unlike DNA, and the LNA-based assay is sensitive to single nucleobase mismatches, the possibilities for label-free in vitro rapid diagnostics based on the LNA probes may be explored. PMID:27025649

  2. An unexpected sequence of events: mismatch detection in the human hippocampus

    Kumaran, D.; Maguire, E. A.


    The ability to identify and react to novelty within the environment is fundamental to survival. Computational models emphasize the potential role of the hippocampus in novelty detection, its unique anatomical circuitry making it ideally suited to act as a comparator between past and present experience. The hippocampus, therefore, is viewed to detect associative mismatches between what is expected based on retrieval of past experience and current sensory input. However, direct evidence that th...

  3. An Unexpected Sequence of Events: Mismatch Detection in the Human Hippocampus

    Kumaran, Dharshan; Maguire, Eleanor A.


    The ability to identify and react to novelty within the environment is fundamental to survival. Computational models emphasize the potential role of the hippocampus in novelty detection, its unique anatomical circuitry making it ideally suited to act as a comparator between past and present experience. The hippocampus, therefore, is viewed to detect associative mismatches between what is expected based on retrieval of past experience and current sensory input. However, direct evidence that th...

  4. Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity.

    Anderson, Emily M; Haupt, Amanda; Schiel, John A; Chou, Eldon; Machado, Hidevaldo B; Strezoska, Žaklina; Lenger, Steve; McClelland, Shawn; Birmingham, Amanda; Vermeulen, Annaleen; Smith, Anja van Brabant


    The discovery that the bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) acquired immune system can be utilized to create double-strand breaks (DSBs) in eukaryotic genomes has resulted in the ability to create genomic changes more easily than with other genome engineering techniques. While there is significant potential for the CRISPR-Cas9 system to advance basic and applied research, several unknowns remain, including the specificity of the RNA-directed DNA cleavage by the small targeting RNA, the CRISPR RNA (crRNA). Here we describe a novel synthetic RNA approach that allows for high-throughput gene editing experiments. This was used with a functional assay for protein disruption to perform high-throughput analysis of crRNA activity and specificity. We performed a comprehensive test of target cleavage using crRNAs that contain one and two nucleotide mismatches to the DNA target in the 20mer targeting region of the crRNA, allowing for the evaluation of hundreds of potential mismatched target sites without the requirement for the off-target sequences and their adjacent PAMs to be present in the genome. Our results demonstrate that while many crRNAs are functional, less than 5% of crRNAs with two mismatches to their target are effective in gene editing; this suggests an overall high level of functionality but low level of off-targeting. PMID:26189696

  5. Mis-Match Limit Load Analyses and Fracture Mechanics Assessment for Welded Pipe with Circumferential Crack at the Center of Weldment

    In this paper, limit load analyses and fracture mechanics analyses were conducted via finite element analyses for the welded pipe with circumferential crack at the center of the weldment. Systematic changes for strength mismatch ratio, width of weldment, crack shape and thickness ratio of the pipe were considered to provide strength mismatch limit load. And J-integral calculations based on reference stress method were conducted for two materials, stainless steel and ferritic steel. Reference stress defined by provided strength mis-match limit load gives much more accurate J-integral

  6. Functional interactions and signaling properties of mammalian DNA mismatch repair proteins.

    Bellacosa, A


    The mismatch repair (MMR) system promotes genomic fidelity by repairing base-base mismatches, insertion-deletion loops and heterologies generated during DNA replication and recombination. This function is critically dependent on the assembling of multimeric complexes involved in mismatch recognition and signal transduction to downstream repair events. In addition, MMR proteins coordinate a complex network of physical and functional interactions that mediate other DNA transactions, such as transcription-coupled repair, base excision repair and recombination. MMR proteins are also involved in activation of cell cycle checkpoint and induction of apoptosis when DNA damage overwhelms a critical threshold. For this reason, they play a role in cell death by alkylating agents and other chemotherapeutic drugs, including cisplatin. Inactivation of MMR genes in hereditary and sporadic cancer is associated with a mutator phenotype and inhibition of apoptosis. In the future, a deeper understanding of the molecular mechanisms and functional interactions of MMR proteins will lead to the development of more effective cancer prevention and treatment strategies. PMID:11687886

  7. Parametric Adaptive Radar Detector with Enhanced Mismatched Signals Rejection Capabilities

    Liu Bin


    Full Text Available We consider the problem of adaptive signal detection in the presence of Gaussian noise with unknown covariance matrix. We propose a parametric radar detector by introducing a design parameter to trade off the target sensitivity with sidelobes energy rejection. The resulting detector merges the statistics of Kelly's GLRT and of the Rao test and so covers Kelly's GLRT and the Rao test as special cases. Both invariance properties and constant false alarm rate (CFAR behavior for this detector are studied. At the analysis stage, the performance of the new receiver is assessed and compared with several traditional adaptive detectors. The results highlight better rejection capabilities of this proposed detector for mismatched signals. Further, we develop two two-stage detectors, one of which consists of an adaptive matched filter (AMF followed by the aforementioned detector, and the other is obtained by cascading a GLRT-based Subspace Detector (SD and the proposed adaptive detector. We show that the former two-stage detector outperforms traditional two-stage detectors in terms of selectivity, and the latter yields more robustness.

  8. Adjacent Instability after Instrumented Lumbar Fusion.

    Wen-Jer Chen


    Full Text Available The invention of pedicle screw instrumentation has greatly improved outcomes ofspinal fusion, which has become the treatment of choice for lumbar spondylolisthesis. Asresearchers accumulate experience, both theoretical and clinical advances are continuallybeing reported. A review of the literature and the experience of the authors show that thedevelopment of adjacent instability, as in the breakdown of a neighboring unfixed motionsegment, is a common consequence of an instrumented lumbar spine. This article reviewsthe risk factors and surgical treatment of adjacent instability. The authors believe that properpreoperative planning and complete surgical procedures are imperative to prevent adjacentinstability. For those who need revision surgery, meticulous surgical techniques can achievesatisfactory results.

  9. On the complexity of adjacent resource scheduling

    Duin, C.W.; Sluis, van, P.


    We study the problem of scheduling resource(s) for jobs in an adjacent manner (ARS). The problem relates to fixed interval scheduling on the one hand, and to the problem of two-dimensional strip packing on the other hand. Further, there is a close relation with multiprocessor scheduling. A distinguishing characteristic is the constraint of resource-adjacency. As an application of ARS, consider an airport where passengers check in for their flight, joining lines before one or more desks; at th...

  10. KAMG: A Tool for Converting Blood Ties and Affinity Ties into Adjacency Matrices

    Hang Xiong


    Full Text Available Kinship Adjacency Matrix Generator (KAMG is a browser-based software for creating adjacency matrices using the information of kinship ties. Specifically, it is capable of converting the family trees in the format of GEDCOM files into adjacency matrices of blood relationship. With the data of intermarriages between different families, it can further create the adjacency matrix of affinity relationship for the families. The outcomes can be directly used to create networks. KAMG is written in JavaScript and implemented on web browsers. It is completely open source and the source code is publicly available on GitHub.

  11. Direct Mismatch Characterization of femto-Farad Capacitors

    Omran, Hesham


    Reducing the capacitance of programmable capacitor arrays, commonly used in analog integrated circuits, is necessary for low-energy applications. However, limited mismatch data is available for small capacitors. We report mismatch measurement for a 2fF poly-insulator-poly (PIP) capacitor, which is the smallest reported PIP capacitor to the best of the authors’ knowledge. Instead of using complicated custom onchip circuitry, direct mismatch measurement is demonstrated and verified using Monte Carlo Simulations and experimental measurements. Capacitive test structures composed of 9 bit programmable capacitor arrays (PCAs) are implemented in a low-cost 0:35m CMOS process. Measured data is compared to mismatch of large PIP capacitors, theoretical models, and recently published data. Measurement results indicate an estimated average relative standard deviation of 0.43% for the 2fF unit capacitor, which is better than the reported mismatch of metal-oxide-metal (MOM) fringing capacitors implemented in an advanced 32nm CMOS process.

  12. Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR

    Seiki Kuramitsu


    Full Text Available Polymerase chain reaction (PCR-related technologies are hampered mainly by two types of error: nonspecific amplification and DNA polymerase-generated mutations. Here, we report that both errors can be suppressed by the addition of a DNA mismatch-recognizing protein, MutS, from a thermophilic bacterium. Although it had been expected that MutS has a potential to suppress polymerase-generated mutations, we unexpectedly found that it also reduced nonspecific amplification. On the basis of this finding, we propose that MutS binds a mismatched primer-template complex, thereby preventing the approach of DNA polymerase to the 3' end of the primer. Our simple methodology improves the efficiency and accuracy of DNA amplification and should therefore benefit various PCR-based applications, ranging from basic biological research to applied medical science.

  13. Mismatch oxidation assay: detection of DNA mutations using a standard UV/Vis microplate reader.

    Tabone, Tania; Sallmann, Georgina; Cotton, Richard G H


    Simple, low-cost mutation detection assays that are suitable for low-throughput analysis are essential for diagnostic applications where the causative mutation may be different in every family. The mismatch oxidation assay is a simple optical absorbance assay to detect nucleotide substitutions, insertions, and deletions in heteroduplex DNA. The method relies on detecting the oxidative modification products of mismatched thymine and cytosine bases by potassium permanganate as it is reduced to manganese dioxide. This approach, unlike other methods commonly used to detect sequence variants, does not require costly labeled probes or primers, toxic chemicals, or a time-consuming electrophoretic separation step. The oxidation rate, and hence the presence of a sequence variant, is detected by measuring the formation of the potassium permanganate reduction product (hypomanganate diester), which absorbs at the 420-nm visible wavelength, using a standard UV/vis microplate reader. PMID:19768598

  14. An analytical model for finite radius dual-beam mode-mismatched thermal lens spectroscopy

    Sabaeian, Mohammad; Nadgaran, Hamid


    In this work, a new model for dual-beam mode-mismatch thermal lens spectroscopy is presented. The model was based on a new analytical solution of time-dependent heat equation for finite radius cylindrical samples exposed to TEM00 excitation laser beams. The Fresnel diffraction integration method was used to calculate time-dependent on-axis probe beam intensity. All aberrations in thermal lens were taken into account. The model yields accurate values for absorption coefficient and thermal diffusivity of methylene blue aqueous solution. Furthermore, the optimized mode-mismatched version of this model when applied to pure water as a very low absorbent yields its absorption coefficient and thermal diffusivity values close to literature data. In contrast to traditional model, this model does not need to omit any term in its theory to fit the experimental data.

  15. Adjacent stimulation and measurement patterns considered harmful

    We characterize the ability of electrical impedance tomography (EIT) to distinguish changes in internal conductivity distributions, and analyze it as a function of stimulation and measurement patterns. A distinguishability measure, z, is proposed which is related to the signal-to-noise ratio of a medium and to the probability of detection of conductivity changes in a region of interest. z is a function of the number of electrodes, the EIT stimulation and measurement protocol, the stimulation amplitude, the measurement noise, and the size and location of the contrasts. Using this measure we analyze various choices of stimulation and measurement patterns under the constraint of medical electrical safety limits (maximum current into the body). Analysis is performed for a planar placement of 16 electrodes for simulated 3D tank and chest shapes, and measurements in a saline tank. Results show that the traditional (and still most common) adjacent stimulation and measurement patterns have by far the poorest performance (by 6.9 ×). Good results are obtained for trigonometric patterns and for pair drive and measurement patterns separated by over 90°. Since the possible improvement over adjacent patterns is so large, we present this result as a call to action: adjacent patterns are harmful, and should be abandoned. We recommend using pair drive and measurement patterns separated by one electrode less than 180°. We describe an approach to modify an adjacent pattern EIT system by adjusting electrode placement

  16. Refractive index and dielectric constants of lattice matched and mismatched Cdx Zn1-x Sey Te1-y quaternary alloys

    We report on a study of optical properties, namely the refractive index and high-frequency and static dielectric constants of zinc-blende Cdx Zn1-x Sey Te1-y under conditions of lattice matching and lattice mismatching to ZnTe substrates. The calculations are mainly based on the pseudopotential approach under a virtual crystal approximation. Our results show that the Cdx Zn1-x Sey Te1-y lattice matched to ZnTe is a direct band-gap semiconductor for all possible values of x and y (0 ≤x≤1, 0≤y≤0.879). The studied features are found to be strongly dependent on the lattice mismatch percentage. The present investigation provides more opportunities to obtain diverse refractive indices and dielectric constants, while still controlling the composition parameters (x and y) and/or the lattice mismatch percentage.

  17. Mismatch repair and treatment resistance in ovarian cancer

    The treatment of ovarian cancer is hindered by intrinsic or acquired resistance to platinum-based chemotherapy. The aim of this study is to determine the frequency of mismatch repair (MMR) inactivation in ovarian cancer and its association with resistance to platinum-based chemotherapy. We determined, microsatellite instability (MSI) as a marker for MMR inactivation (analysis of BAT25 and BAT26), MLH1 promoter methylation status (methylation specific PCR on bisulfite treated DNA) and mRNA expression of MLH1, MSH2, MSH3, MSH6 and PMS2 (quantitative RT-PCR) in 75 ovarian carcinomas and eight ovarian cancer cell lines MSI was detected in three of the eight cell lines i.e. A2780 (no MLH1 mRNA expression due to promoter methylation), SKOV3 (no MLH1 mRNA expression) and 2774 (no altered expression of MMR genes). Overall, there was no association between cisplatin response and MMR status in these eight cell lines. Seven of the 75 ovarian carcinomas showed MLH1 promoter methylation, however, none of these showed MSI. Forty-six of these patients received platinum-based chemotherapy (11 non-responders, 34 responders, one unknown response). The resistance seen in the eleven non-responders was not related to MSI and therefore also not to MMR inactivation. No MMR inactivation was detected in 75 ovarian carcinoma specimens and no association was seen between MMR inactivation and resistance in the ovarian cancer cell lines as well as the ovarian carcinomas. In the discussion, the results were compared to that of twenty similar studies in the literature including in total 1315 ovarian cancer patients. Although no association between response and MMR status was seen in the primary tumor the possible role of MMR inactivation in acquired resistance deserves further investigation

  18. Soil Structure Interaction between Two Adjacent Buildings under Earthquake Load

    Mahmoud Yahyai; Masoud Mirtaheri; Mehrab Mahoutian; Amir S. Daryan


    In some cases, tall buildings are located in geotechnically unsuitable places, due to their high ratio of height to width; there is risk of uplift and other effects such as overturning and reduction structure serviceability during earthquake. This research is aimed to evaluate the effect of Soil-Structure Interaction (SSI) on seismic behavior of two adjacent 32 story buildings such as time period, base shear and displacements. The interaction effects are investigated for variable distance bet...

  19. Disrupted Turn Adjacency and Coherence Maintenance in Instant Messaging Conversations

    Berglund, Therese Örnberg


    This article reports on a study of coherence in text-based log files from 120 two-party Instant Messaging (IM) conversations among a group of international students at a design school. The goals of the study were to investigate whether disrupted turn adjacency was an obstacle to coherence and to identify the linguistic strategies employed to maintain coherence. Additional signs of problematic coherence creation were also investigated, focusing on explicit signs of miscommunication. In this pa...

  20. Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains

    Milovanovic, Dragomir; Honigmann, Alf; Koike, Seiichi; Göttfert, Fabian; Pähler, Gesa; Junius, Meike; Müllar, Stefan; Diederichsen, Ulf; Janshoff, Andreas; Grubmüller, Helmut; Risselada, Herre J.; Eggeling, Christian; Hell, Stefan W.; van den Bogaart, Geert; Jahn, Reinhard


    The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic and ionic interactions with membrane lipids as well as of specific protein-protein interactions. Here using plasma membrane-resident SNARE proteins as model, we show that hydrophobic mismatch between the length of transmembrane domains (TMDs) and the thickness of the lipid membrane suffices to induce clustering of proteins. Even when the TMDs differ in length by only a single residue, hydrophobic mismatch can segregate structurally closely homologous membrane proteins in distinct membrane domains. Domain formation is further fine-tuned by interactions with polyanionic phosphoinositides and homo and heterotypic protein interactions. Our findings demonstrate that hydrophobic mismatch contributes to the structural organization of membranes.

  1. Bilayer thickness mismatch controls domain size in biomimetic membranes

    Heberle, Frederick A.; Petruzielo, Robin S.; Pan, Jianjun; Drazba, Paul; Kučerka, Norbert; Standaert, Robert F.; Feigenson, Gerald W.; Katsara, John


    In order to promote functionality, cells may alter the spatial organization of membrane lipids and proteins, including separation of liquid phases into distinct domains. In model membranes, domain size and morphology depend strongly on composition and temperature, but the physicochemical mechanisms controlling them are poorly understood. Theoretical work suggests a role for interfacial energy at domain boundaries, which may be driven in part by thickness mismatch between a domain and its surrounding bilayer. However, no direct evidence linking thickness mismatch to domain size in free-standing bilayers has been reported. We describe the use of Small Angle Neutron Scattering (SANS) to detect domains in simplified lipid-only models that mimic the composition of plasma membrane. We find that domain size is controlled by the degree of acyl chain unsaturation of low-melting temperature lipids, and that this size transition is correlated to changes in the thickness mismatch between coexisting liquid phases.

  2. Interobserver variability in the evaluation of mismatch repair protein immunostaining

    Klarskov, Louise; Ladelund, Steen; Holck, Susanne; Roenlund, Karina; Lindebjerg, Jan; Elebro, Jacob; Halvarsson, Britta; von Salomé, Jenny; Bernstein, Inge; Nilbert, Mef


    reliability are lacking. We therefore evaluated interobserver variability among 6 pathologists, 3 experienced gastrointestinal pathologists and 3 residents. In total, 225 immunohistochemically stained colorectal cancers were evaluated as having normal, weak, loss of, or nonevaluable mismatch repair protein......Immunohistochemical staining for mismatch repair proteins has during recent years been established as a routine analysis in many pathology laboratories with the aim to identify tumors linked to the hereditary nonpolyposis colorectal cancer syndrome. Despite widespread application, data on...... variability was considerable, though experienced pathologists and residents reached the same level of consensus. Because results from immunohistochemical mismatch repair protein stainings are used for decisions on mutation analysis and as an aid in the interpretation of gene variants of unknown significance...

  3. Interobserver variability in the evaluation of mismatch repair protein immunostaining

    Klarskov, Louise Laurberg; Ladelund, Steen; Holck, Susanne;


    reliability are lacking. We therefore evaluated interobserver variability among 6 pathologists, 3 experienced gastrointestinal pathologists and 3 residents. In total, 225 immunohistochemically stained colorectal cancers were evaluated as having normal, weak, loss of, or nonevaluable mismatch repair protein...... variability was considerable, though experienced pathologists and residents reached the same level of consensus. Because results from immunohistochemical mismatch repair protein stainings are used for decisions on mutation analysis and as an aid in the interpretation of gene variants of unknown significance...... in hereditary nonpolyposis colorectal cancer, the interobserver variability identified highlights the need for quality assessment programs, including guidelines for classification of different expression patterns....


    Sun Xiaowen; Zhang Linrang; Wu Shunjun


    This letter demonstrates the structure of the passive radar using TV signals. Because the TV signal is a kind of pseudoperiodic signal, the matched filtering of color TV signals would yield high sidelobes which cause the range ambiguity. To overcome this problem, the mismatched filter is proposed to suppress the correlation sidelobes of matched filtering of TV signals. By utilizing the iteration process, this method could achieve the required peak sidelobe level. The impacts of the noise and target movement on mismatched filtering are also analysed. Simulation results are included to demonstrate the effectiveness of the proposed technique.

  5. Advanced radar detection schemes under mismatched signal models

    Bandiera, Francesco


    Adaptive detection of signals embedded in correlated Gaussian noise has been an active field of research in the last decades. This topic is important in many areas of signal processing such as, just to give some examples, radar, sonar, communications, and hyperspectral imaging. Most of the existing adaptive algorithms have been designed following the lead of the derivation of Kelly's detector which assumes perfect knowledge of the target steering vector. However, in realistic scenarios, mismatches are likely to occur due to both environmental and instrumental factors. When a mismatched signal

  6. Visual mismatch negativity reveals automatic detection of sequential regularity violation

    Gábor Stefanics


    Full Text Available Sequential regularities are abstract rules based on repeating sequences of environmental events, which are useful to make predictions about future events. As the processes underlying visual mismatch negativity (vMMN are sensitive to complex stimulus changes, this event-related potential component, like its auditory counterpart, may be an index of a primitive system of intelligence. Here we tested whether the visual system is capable to detect abstract sequential regularity in unattended stimulus sequences. In our first experiment we investigated the emergence of vMMN and other change-related activity to stimuli violating abstract rules. Red and green disk patterns were delivered in pairs. When in the majority of pairs the colors were identical within the pairs, deviant pairs with different colors for the second member of the pair elicited vMMN. Spatially more extended vMMN responses with longer latency were observed for deviants with 10% compared to 30% probability. In our second experiment utilizing oddball sequences, we tested the emergence of vMMN to violations of a concrete, feature-based rule of a repetition of a standard color. Deviant colors elicited a vMMN response in the oddball sequences. VMMN was larger for the second member of the pair, i.e. after a shorter stimulus onset asynchrony (SOA. This result corresponds to the expected SOA/(vMMN relationship. Our results show that the system underlying vMMN is sensitive to abstract probability rules and this component can be considered as a correlate of violated predictions about the characteristics of environmental events.

  7. A Provably Efficient Algorithm for the k-Mismatch Average Common Substring Problem.

    Thankachan, Sharma V; Apostolico, Alberto; Aluru, Srinivas


    Alignment-free sequence comparison methods are attracting persistent interest, driven by data-intensive applications in genome-wide molecular taxonomy and phylogenetic reconstruction. Among all the methods based on substring composition, the average common substring (ACS) measure admits a straightforward linear time sequence comparison algorithm, while yielding impressive results in multiple applications. An important direction of this research is to extend the approach to permit a bounded edit/hamming distance between substrings, so as to reflect more accurately the evolutionary process. To date, however, algorithms designed to incorporate k ≥ 1 mismatches have O(n(2)) worst-case time complexity, where n is the total length of the input sequences. On the other hand, accounting for mismatches has shown to lead to much improved classification, while heuristics can improve practical performance. In this article, we close the gap by presenting the first provably efficient algorithm for the k-mismatch average common string (ACSk) problem that takes O(n) space and O(n log(k) n) time in the worst case for any constant k. Our method extends the generalized suffix tree model to incorporate a carefully selected bounded set of perturbed suffixes, and can be applied to other complex approximate sequence matching problems. PMID:27058840

  8. Cross-scale feedbacks and scale mismatches as influences on cultural services and the resilience of protected areas.

    Maciejewski, Kristine; De Vos, Alta; Cumming, Graeme S; Moore, Christine; Biggs, Duan


    Protected areas are a central strategy for achieving global conservation goals, but their continued existence depends heavily on maintaining sufficient social and political support to outweigh economic interests or other motives for land conversion. Thus, the resilience of protected areas can be considered a function of their perceived benefits to society. Nature-based tourism (NBT), a cultural ecosystem service, provides a key source of income to protected areas, facilitating a sustainable solution to conservation. The ability of tourism to generate income depends, however, on both the scales at which this cultural service is provided and the scales at which tourists respond to services on offer. This observation raises a set of location-, context-, and scale-related questions that need to be confronted before we can understand and value cultural service provision appropriately. We combine elements of resilience analysis with a systems ecology framework and apply this to NBT in protected areas to investigate cross-scale interactions and scale mismatches. We postulate that cross-scale effects can either have a positive effect on protected area resilience or lead to scale mismatches, depending on their interactions with cross-scale feedbacks. To demonstrate this, we compare spatial scales and nested levels of institutions to develop a typology of scale mismatches for common scenarios in NBT. In our new typology, the severity of a scale mismatch is expressed as the ratio of spatial scale to institutional level, producing 25 possible outcomes with differing consequences for system resilience. We predict that greater differences between interacting scales and levels, and greater magnitudes of cross-scale interactions, will lead to greater magnitudes of scale mismatch. Achieving a better understanding of feedbacks and mismatches, and finding ways of aligning spatial and institutional scales, will be critical for strengthening the resilience of protected areas that

  9. Adjacent Segment Pathology after Lumbar Spinal Fusion.

    Lee, Jae Chul; Choi, Sung-Woo


    One of the major clinical issues encountered after lumbar spinal fusion is the development of adjacent segment pathology (ASP) caused by increased mechanical stress at adjacent segments, and resulting in various radiographic changes and clinical symptoms. This condition may require surgical intervention. The incidence of ASP varies with both the definition and methodology adopted in individual studies; various risk factors for this condition have been identified, although a significant controversy still exists regarding their significance. Motion-preserving devices have been developed, and some studies have shown their efficacy of preventing ASP. Surgeons should be aware of the risk factors of ASP when planning a surgery, and accordingly counsel their patients preoperatively. PMID:26435804

  10. Educational Mismatch and Spatial Flexibility in Italian Local Labour Markets

    Croce, Giuseppe; Ghignoni, Emanuela


    According to recent literature, this paper highlights the relevance of spatial mobility as an explanatory factor of the individual risk of job-education mismatch. To investigate this causal link, we use individual information about daily home-to-work commuting time and choices to relocate in a different local area to get a job. Our model takes…

  11. The dual nature of mismatch repair as antimutator and mutator

    Bak, Sara Thornby; Sakellariou, Despoina; Pena Diaz, Javier


    DNA is constantly under attack by a number of both exogenous and endogenous agents that challenge its integrity. Among the mechanisms that have evolved to counteract this deleterious action, mismatch repair (MMR) has specialized in removing DNA biosynthetic errors that occur when replicating the...

  12. Highly tunable mismatched twin-core fibre filters

    Ortega, B.; Dong, L.


    We demonstrated a reproducible and accurate way for adjusting wavelength of mismatched twin-core filters over few hundred nanometres from the same fibre design. The wavelength control together with strength control by fibre length adjustment allows complex filter profiles to be implemented by cascading. These filters are highly stable to changes in temperature and strain.

  13. Investigating Interaural Frequency-Place Mismatches via Bimodal Vowel Integration

    Guérit, François; Santurette, Sébastien; Chalupper, Josef;


    For patients having residual hearing in one ear and a cochlear implant (CI) in the opposite ear, interaural place-pitch mismatches might be partly responsible for the large variability in individual benefit. Behavioral pitch-matching between the two ears has been suggested as a way to individuali...... difficulties in bimodal fusion of the two formants....

  14. Mismatch-shaping switching for two-capacitor DAC

    Steensgaard-Madsen, Jesper; Moon, U.; Temes, G.C.


    A mismatch-shaping scheme is proposed for a two-capacitor digital-to-analogue converter (DAC). It uses a delta-sigma loop for finding the optimal switching sequence for each input word. Simulations indicate that the scheme can be used for the realisation of DACs with 16 bit linearity and SNR perf...

  15. Feature Mismatch: Deponency in Indo-European Languages

    Grestenberger, Laura


    This thesis investigates "voice mismatch verbs" (deponents), verbs that take non-active morphology but are used in syntactically active environments. The focus is on the non-informant Indo-European languages Hittite, Vedic Sanskrit, Ancient Greek, and Latin, supplemented by data from Modern Greek.

  16. Georgia : Skills Mismatch and Unemployment, Labor Market Challenges

    World Bank


    This paper reviews labor market performance in Georgia, and examines the link between labor market outcomes and poverty. At over 15 percent, the unemployment rate in the country is high by European standards. Georgia faces three main labor market issues: (a) underutilization of labor resources, (b) earnings inequality, and (c) skills mismatch. All three have a negative impact on poverty. T...

  17. Mismatch of Vocational Graduates: What Penalty on French Labour Market?

    Beduwe, Catherine; Giret, Jean-Francois


    This study explores individual effects of educational mismatch on wages, job satisfaction and on-the-job-search on French labour market. We distinguish between horizontal matches (job matches with field of studies) and vertical matches (job matches the level of qualification) on the one hand and skills matches (worker's assessment) on the other…

  18. Mismatched feature detection with finer granularity for emotional speaker recognition

    Li CHEN; Ying-chun YANG; Zhao-hui WU


    The shapes of speakers’ vocal organs change under their different emotional states, which leads to the deviation of the emotional acoustic space of short-time features from the neutral acoustic space and thereby the degradation of the speaker recognition performance. Features deviating greatly from the neutral acoustic space are considered as mismatched features, and they negatively affect speaker recognition systems. Emotion variation produces different feature deformations for different phonemes, so it is reasonable to build a fi ner model to detect mismatched features under each phoneme. However, given the difficulty of phoneme recognition, three sorts of acoustic class recognition- phoneme classes, Gaussian mixture model (GMM) tokenizer, and probabilistic GMM tokenizer- are proposed to replace phoneme recognition. We propose feature pruning and feature regulation methods to process the mismatched features to improve speaker recognition performance. As for the feature regulation method, a strategy of maximizing the between-class distance and minimizing the within-class distance is adopted to train the transformation matrix to regulate the mismatched features. Experiments conducted on the Mandarin affective speech corpus (MASC) show that our feature pruning and feature regulation methods increase the identifi cation rate (IR) by 3.64% and 6.77%, compared with the baseline GMM-UBM (universal background model) algorithm. Also, corresponding IR increases of 2.09% and 3.32% can be obtained with our methods when applied to the state-of-the-art algorithm i-vector.

  19. Bisimulation Lattice of Asymmetric Chi Calculus with Mismatch

    Dong Xiaoju(董笑菊); Zhong Farong; Fu Yuxi


    This paper carries out a systematic investigation into the bisimulation lattice of asymmetric chi calculus with a mismatch combinator. It is shown that all the sixty three L-bisimilarities collapse to twelve distinct relations and they form a bisimulation lattice with respect to set inclusion. The top of the lattice coincides with the barbed bisimilarity.

  20. Clinicopathologic factors identify sporadic mismatch repair-defective colon cancers

    Halvarsson, Britta; Anderson, Harald; Domanska, Katarina; Lindmark, Gudrun; Nilbert, Mef


    Identification of sporadic mismatch repair (MMR)-defective colon cancers is increasingly demanded for decisions on adjuvant therapies. We evaluated clinicopathologic factors for the identification of these prognostically favorable tumors. Histopathologic features in 238 consecutive colon cancers...... and excluded 61.5% of the tumors from MMR testing. This clinicopathologic index thus successfully selects MMR-defective colon cancers. Udgivelsesdato: 2008-Feb...

  1. Does the temporal mismatch hypothesis match in boreal populations?

    Vatka, Emma; Rytkönen, Seppo; Orell, Markku


    The temporal mismatch hypothesis suggests that fitness is related to the degree of temporal synchrony between the energetic needs of the offspring and their food supply. The hypothesis has been a basis in studying the influence of climate warming on nature. This study enhances the knowledge on prevalence of temporal mismatches and their consequences in boreal populations, and questions the role of the temporal mismatch hypothesis as the principal explanation for the evolution of timing of breeding. To test this, we examined if synchrony with caterpillar prey or timing of breeding per se better explains reproductive output in North European parid populations. We compared responses of temperate-origin species, the great tit (Parus major) and the blue tit (Cyanistes caeruleus), and a boreal species, the willow tit (Poecile montanus). We found that phenologies of caterpillars and great tits, but not of blue tits, have advanced during the past decades. Phenologies correlated with spring temperatures that may function as cues about the timing of the food peak for great and blue tits. The breeding of great and blue tits and their caterpillar food remained synchronous. Synchrony explained breeding success better than timing of breeding alone. However, the synchrony effect arose only in certain conditions, such as with high caterpillar abundances or high breeding densities. Breeding before good synchrony seems advantageous at high latitudes, especially in the willow tit. Thus, the temporal mismatch hypothesis appears insufficient in explaining the evolution of timing of breeding. PMID:25024104

  2. Expectations and Outcomes of Vocational Education: Match or Mismatch.

    Lotto, Linda S.


    This article provides an initial look at the matches and mismatches between expectations held for vocational education programs and empirically observed outcomes. Three types of claims for vocational education were compared with recent research. The article concludes with recommendations for improving the alignment between expectations and…

  3. 基于混合居住的城市毗邻隔离住区更新%Renewal of the Urban Adjacent Segregated Residential Area Based on Mixed-Income Residence

    杨昌鸣; 张祥智; 李湘桔


    将混合居住理论应用于既有住区更新研究中,在对城市中普遍存在的毗邻隔离住区类型进行概念界定与特征剖析的基础上,提出了互融更新的策略,并尝试在微观层面上深化混合居住理论,增进混合的实效性.%Applying the theory of mixed-income residence to the renewal of existing residential area, and by the concept definition and characteristics analysis, this paper proposes the tactics of mutual fusion for the adjacent and segregated residential area, which is commonly existed in city. It tries to deepen the theory of mixed-income residence at microievel, and to enhance the effectiveness of the mixed-composite.

  4. Band anticrossing effects in highly mismatched semiconductor alloys

    Wu, Junqiao


    The first five chapters of this thesis focus on studies of band anticrossing (BAC) effects in highly electronegativity- mismatched semiconductor alloys. The concept of bandgap bowing has been used to describe the deviation of the alloy bandgap from a linear interpolation. Bowing parameters as large as 2.5 eV (for ZnSTe) and close to zero (for AlGaAs and ZnSSe) have been observed experimentally. Recent advances in thin film deposition techniques have allowed the growth of semiconductor alloys composed of significantly different constituents with ever- improving crystalline quality (e.g., GaAs{sub 1-x}N{sub x} and GaP{sub 1-x}N{sub x} with x {approx}< 0.05). These alloys exhibit many novel and interesting properties including, in particular, a giant bandgap bowing (bowing parameters > 14 eV). A band anticrossing model has been developed to explain these properties. The model shows that the predominant bowing mechanism in these systems is driven by the anticrossing interaction between the localized level associated with the minority component and the band states of the host. In this thesis I discuss my studies of the BAC effects in these highly mismatched semiconductors. It will be shown that the results of the physically intuitive BAC model can be derived from the Hamiltonian of the many-impurity Anderson model. The band restructuring caused by the BAC interaction is responsible for a series of experimental observations such as a large bandgap reduction, an enhancement of the electron effective mass, and a decrease in the pressure coefficient of the fundamental gap energy. Results of further experimental investigations of the optical properties of quantum wells based on these materials will be also presented. It will be shown that the BAC interaction occurs not only between localized states and conduction band states at the Brillouin zone center, but also exists over all of k-space. Finally, taking ZnSTe and ZnSeTe as examples, I show that BAC also occurs between

  5. A Statistic-Based Calibration Method for TIADC System

    Kuojun Yang


    Full Text Available Time-interleaved technique is widely used to increase the sampling rate of analog-to-digital converter (ADC. However, the channel mismatches degrade the performance of time-interleaved ADC (TIADC. Therefore, a statistic-based calibration method for TIADC is proposed in this paper. The average value of sampling points is utilized to calculate offset error, and the summation of sampling points is used to calculate gain error. After offset and gain error are obtained, they are calibrated by offset and gain adjustment elements in ADC. Timing skew is calibrated by an iterative method. The product of sampling points of two adjacent subchannels is used as a metric for calibration. The proposed method is employed to calibrate mismatches in a four-channel 5 GS/s TIADC system. Simulation results show that the proposed method can estimate mismatches accurately in a wide frequency range. It is also proved that an accurate estimation can be obtained even if the signal noise ratio (SNR of input signal is 20 dB. Furthermore, the results obtained from a real four-channel 5 GS/s TIADC system demonstrate the effectiveness of the proposed method. We can see that the spectra spurs due to mismatches have been effectively eliminated after calibration.

  6. Adjacency effect estimation by ground spectra measurement and satellite optical sensor synchronous observation data

    Jianwen Ma; Xue Chen


    @@ This paper addresses the estimation of adjacency effect of CBERS-2 image.The adjacency effect influences the digital number (DN) value of a pixel by adding surrounding scattering signals and path scattering signals.Based on the theory of radiation transfer model,a procedure is designed to measure the reflectance from the surface target materials and the materials in a box,which is 1.5 m above the surface to avoid upwelling reflectance.The results show that the adjacency effect varies from visible,near infrared and becomes steady within short infrared wavelength region;the adjacency effect weakens with the increase of distance between testing sites.The adjacency effect of CBERS-2 image is corrected and the quality of the resulting image is improved.

  7. Achieving good environmental status in the Black Sea: scale mismatches in environmental management

    Tim O'Higgins


    Full Text Available The Black Sea has suffered severe environmental degradation. Governance of the Black Sea region is complex and results in a series of scale mismatches which constrain management. This paper develops a simple classification of spatial scale mismatches incorporating the driver, pressure, state, welfare, response (DPSWR framework. The scale mismatch classification is applied to two major environmental problems of the Black Sea, eutrophication and small pelagic fisheries. A number of scale mismatches are described and classified and potential solutions are identified.

  8. Policy Debate | Education and Employment Mismatch

    Mona Mourshed


    Full Text Available Editor’s note: These papers are contributions to the ‘Policy Debate’ section of International Development Policy. In this section, academics, policy-makers and practioners engage in a dialogue on global development challenges. Papers are copy-edited but not peer-reviewed. Instead, the initial thematic contribution is followed by critical comments and reactions from scholars and/or policy-makers.Authored by McKinsey’s research team, the initial paper addresses the Education to Employment challenge. It is based on McKinsey’s study, which looked at skill development in 25 different countries and investigated education-to-employment initiatives. The authors claim that the most successful efforts are those where different stakeholders interact intensively and frequently. Employers need to get involved in education, and educators should play a bigger role in employment.The paper is followed by critical comments by two authors, Beatriz Cardoso, Executive Director of Laboratório de Educação, Brazil, and Shailaja Fennell lecturer at the Centre of Development Studies, University of Cambridge, UK.This debate can be pursued on the Journal’s blog

  9. Rapid identification of a major diffusion/perfusion mismatch in distal internal carotid artery or middle cerebral artery ischemic stroke

    Hakimelahi Reza


    Full Text Available Abstract Background We tested the hypothesis that in patients with occlusion of the terminal internal carotid artery and/or the proximal middle cerebral artery, a diffusion abnormality of 70 ml or less is accompanied by a diffusion/perfusion mismatch of at least 100%. Methods Sixty-eight consecutive patients with terminal ICA and/or proximal MCA occlusions and who underwent diffusion/perfusion MRI within 24 hours of stroke onset were retrospectively identified. DWI and mean transit time (MTT volumes were measured. Prospectively, 48 consecutive patients were identified with the same inclusion criteria. DWI and time to peak (TTP lesion volumes were measured. A large mismatch volume was defined as an MTT or TTP abnormality at least twice the DWI lesion volume. Results In the retrospective study, 49 of 68 patients had a DWI lesion volume ≤ 70 ml (mean 20.2 ml; SEM 2.9 ml. A DWI/MTT mismatch of > 100% was observed in all 49 patients (P  100% was present in all 35 (P  Conclusions Acute stroke patients with major anterior circulation artery occlusion are exceedingly likely to have a major diffusion/perfusion mismatch if the diffusion lesion volume is 70 ml or less. This suggests that physiology-based patient assessments may be made using only vessel imaging and diffusion MRI as a simple alternative to perfusion imaging.

  10. Prediction of Steady-State Stresses within Heat Affected Zone Due to Creep Mismatch in Welded Straight Pipes

    This paper reports the steady-state stresses within the heat affected zone (Haz) of a welded straight pipe subject to creep. The creep constants and exponent are varied systematically to see the effect of various mismatches in creep properties on the steady-state creep stresses, via detailed two-dimensional finite element (FE) creep analyses. The weldments consist of the base metal and weld metal with the Haz, which are characterized using the idealized power creep laws with the same creep exponent. The internal pressure and axial loading are considered to see the effect of the loading mode. To quantify the creep stresses, a creep mismatch factor is introduced as a function of the creep constants and exponent. It is concluded that the ratio of the section-averaged stresses for a mismatched case to those for an even matched case are linearly dependent on the mismatch factor. The results are compared with the FE results, including the Type IV region, as well as the R procedure

  11. A Calibrated Charge Pump for Mismatch Reduction in PLL

    Jianhui Wu


    Full Text Available An improved charge pump (CP for phase locked loop (PLL applications is presented. The proposed charge pump circuitry employs a variable current source in its sink path, which realizes feedback network for calibration. This scheme of charge pump minimizes mismatch between the sourcing current and the sinking current efficiently. The circuit is simulated in 0.18um CMOS technology and the simulation results show that good current matching characteristics can be achieved. The mismatch between the sourcing current and the sinking current can be reduced to less than 0.01% and the range of charge pump output voltage varies from 0.3V to 1.5V.

  12. Experimental study of proton beam halo in mismatched beams

    We report measurements of transverse beam-halo formation in mismatched proton beams in a 52-quadrupole FODO-transport channel following the 6.7 MeV RFQ at the Low-Energy Demonstration Accelerator (LEDA) at Los Alamos. Beam profiles in both transverse planes were measured using a new diagnostic device that consists of a movable carbon filament for measurement of the beam core, and scraper plates for measurement of the outer part of the distributions. The initial results indicate a surprisingly strong growth rate of the rms emittance even for the modest space-charge tune depressions of the experiment. Our results are consistent with the complete transfer of free energy of the mismatched beams into emittance growth within 10 envelope oscillations for both the breathing and the quadrupole modes.

  13. A new acoustic mismatch theory for Kapitsa resistance

    This paper generalizes the well-known acoustic mismatch theory of Kapitsa interface thermal resistance by taking into consideration a broad class of thermal vibrations that were excluded from that theory by the imposition of the Sommerfeld radiation condition, which is required for the theory of sound but is not relevant for the analysis of heat transport. This extension preserves the main ideas of the acoustic mismatch theory but provides much more reasonable estimates for the interface resistance. The predictions of the new theory are compared with various published experimental results for the thermal resistance between liquid helium at low temperatures and several different metals (Ag, Au, Cu, Pb and Pt). The computations are straightforward and require only well-known material parameters. The predictions agree with the experiments to within their stated range of accuracy.

  14. Graduate Labour Market Mismatches: New Features Of An Older Matter

    Mihai KORKA


    Full Text Available In the knowledge society progress depends on innovation, on the knowledge acquired through education, on the science put in practice with the purpose of improving the natural, economic, technical and social condition of human life. Universities are in that favoured position of keeping and creating science, of training through and for science. But, universities face today a series of challenges originated in their relation with the state, with the actors from the national and international extra-university environment, in particular with those active in the labour market. The article explores a variety of education mismatches in the graduate labour market: from over-education to skill mismatches and their impact on the employability. It also focuses on the expected improvement of quality management and of the shared responsibilities for the employability of graduates in the interaction between universities and employers.

  15. Interplay between quantum confinement and dielectric mismatch for ultrashallow dopants

    Mol, J. A.; Salfi, J.; Miwa, J. A.; Simmons, M. Y.; Rogge, S.


    Understanding the electronic properties of dopants near an interface is a critical challenge for nanoscale devices. We have determined the effect of dielectric mismatch and quantum confinement on the ionization energy of individual acceptors beneath a hydrogen passivated silicon (100) surface. While dielectric mismatch between the vacuum and the silicon at the interface results in an image charge which enhances the binding energy of subsurface acceptors, quantum confinement is shown to reduce the binding energy. Using scanning tunneling spectroscopy we measure resonant transport through the localized states of individual acceptors. Thermal broadening of the conductance peaks provides a direct measure for the absolute energy scale. Our data unambiguously demonstrates that these two independent effects compete with the result that the ionization energy is less than 5 meV lower than the bulk value for acceptors less than a Bohr radius from the interface.

  16. Three-dimensional visualization of intracranial vessels and adjacent structures

    MR three-dimensional-Fourier-transform, gradient-echo sequences are available that allow the acquisition of high-quality data sets, suitable for three- dimensional image processing. Most recently, flow- compensated measurement sequences are used to achieve a three-dimensional perspective of the vascular anatomy. Spatial relationships between vessels and adjacent structures can be revealed by three-dimensional displays, showing both vessels and tissue surfaces (brain, tumor) in a three- dimensional manner. The image-processing techniques are based on the ray-tracing principle. The method can be used for the optimization of neurosurgical planning in the sellar region

  17. The kernel of the adjacency matrix of a rectangular mesh

    Tomei, Carlos; Vieira, Tania


    Given an m x n rectangular mesh, its adjacency matrix A, having only integer entries, may be interpreted as a map between vector spaces over an arbitrary field K. We describe the kernel of A: it is a direct sum of two natural subspaces whose dimensions are equal to $\\lceil c/2 \\rceil$ and $\\lfloor c/2 \\rfloor$, where c = gcd (m+1,n+1) - 1. We show that there are bases to both vector spaces, with entries equal to 0, 1 and -1. When K = Z/(2), the kernel elements of these subspaces are described...

  18. Single-mode quantum properties of the codirectional Kerr nonlinear coupler: frequency mismatch and exact solution

    In this paper, we investigate the single-mode quantum properties of the codirectional Kerr nonlinear coupler when the frequency mismatch is involved and a condition for an exact solution of equations of motion is fulfilled. Particularly, we investigate quadrature and principal squeezing, Wigner function, quadrature distribution, phase distribution and phase variance. We show that the quadrature squeezing and the phase variance can exhibit collapse-revival and collapse-revival-subrevival phenomena, respectively, based on the values of the detuning parameter. Furthermore, we analytically demonstrate that the system can generate cat states, in particular, Yurke-Stoler states

  19. Intraocular/Intracranial pressure mismatch hypothesis for visual impairment syndrome in space.

    Zhang, Li-Fan; Hargens, Alan R


    Visual impairment intracranial pressure syndrome (VIIP) is considered a major risk for future human spaceflight. Loss of hydrostatic pressure gradients in vascular and cerebrospinal fluid systems due to the removal of gravity associated with subsequent intracranial and intraocular fluid shifts and the resulting intraocular/intracranial pressure mismatch might be important etiology factors causingVIIP syndrome. Acclimation changes in the ocular and cerebral circulation and the two fluid systems during chronic microgravity exposure and their underlying mechanisms need further elucidation. Relevant findings may help to validate the pressure differential hypothesis for VlIP syndrome and to evaluate whether a gravity based countermeasure is needed. PMID:24479265

  20. Rad9 plays an important role in DNA mismatch repair through physical interaction with MLH1

    He, Wei; Zhao, Yun; Zhang, Chunbo; An, Lili; Hu, Zhishang; Liu, Yuheng; Han, Lu; Bi, Lijun; Xie, Zhensheng; Xue, Peng; Yang, Fuquan; Hang, Haiying


    Rad9 is conserved from yeast to humans and plays roles in DNA repair (homologous recombination repair, and base-pair excision repair) and cell cycle checkpoint controls. It has not previously been reported whether Rad9 is involved in DNA mismatch repair (MMR). In this study, we have demonstrated that both human and mouse Rad9 interacts physically with the MMR protein MLH1. Disruption of the interaction by a single-point mutation in Rad9 leads to significantly reduced MMR activity. This disrup...

  1. Robust Feedback Controller for Exponential Stability of Nonlinear Systems with Mismatched Uncertainties


    The robust stabilization of nonlinear systems with mismatched uncertainties is investigated. Based on the stability of the nominal system, a new approach to synthesizing a class of continuous state feedback controllers for uncertain nonlinear dynamical systems is proposed. By such feedback controllers, the exponential stability of uncertain nonlinear dynamical systems can be guaranteed. The approach can give a clear insight to system analysis. An illustrative example is given to demonstrate the utilization of the approach developed. Simulation results show that the method presented is practical and effective.

  2. Energy Detection of Multilevel PAM Signals with Systematic Threshold Mismatch

    Antti Anttonen


    Full Text Available We address a symbol decision problem with spectrally efficient energy detected multilevel pulse amplitude modulated (PAM signals. First, we analytically quantify the relationship between a systematic threshold mismatch and the required increase of the average signal-to-noise ratio to preserve a desired symbol error rate. For the case in which such an increase is not tolerable, we present a novel near-optimal multilevel threshold selection scheme, which is accurate for a wide range of system parameters.

  3. Race, Segregation, and Postal Employment: New Evidence on Spatial Mismatch

    Leah Platt Boustan; Margo, Robert A.


    The spatial mismatch hypothesis posits that employment decentralization isolated urban blacks from work opportunities. This paper focuses on one large employer that has remained in the central city over the twentieth century - the U.S. Postal Service. We find that blacks substitute towards postal work as other employment opportunities leave the city circa 1960. The response is particularly strong in segregated areas, where black neighborhoods are clustered near the central business district. ...

  4. Unconscious learning of auditory discrimination using mismatch negativity (MMN) neurofeedback

    Ming Chang; Hiroyuki Iizuka; Yasushi Naruse; Hideyuki Ando; Taro Maeda


    Neurofeedback is a strong direct training method for brain function, wherein brain activity patterns are measured and displayed as feedback, and trainees try to stabilize the feedback signal onto certain desirable states to regulate their own mental states. Here, we introduce a novel neurofeedback method, using the mismatch negativity (MMN) responses elicited by similar sounds that cannot be consciously discriminated. Through neurofeedback training, without participants' attention to the audi...

  5. Education Policy, Occupation-Mismatch and the Skill Premium

    Francesc Obiols-Homs; Virginia Sánchez-Marcos


    A relatively low tertiary education wage premium and a large occupational mismatch are two salient features of the Spanish labor market that distinguish it with respect to the labor markets in other developed countries. In this paper we provide an equilibrium model of the labor market with frictions in which workers are heterogeneous in terms of ability and education. We specifically model an education policy as delivering either a particular selection of individuals into the tertiary educati...

  6. Pathological assessment of mismatch repair gene variants in Lynch syndrome

    Rasmussen, Lene Juel; Heinen, Christopher D; Royer-Pokora, Brigitte; Drost, Mark; Tavtigian, Sean; Hofstra, Robert M W; de Wind, Niels


    Lynch syndrome (LS) is caused by germline mutations in DNA mismatch repair (MMR) genes and is the most prevalent hereditary colorectal cancer syndrome. A significant proportion of variants identified in MMR and other common cancer susceptibility genes are missense or noncoding changes whose...... attention to common misconceptions about functional assays and endorse development of an integrated approach comprising validated assays for diagnosis of VUS in patients suspected of LS....

  7. Islamic Finance:Structure-objective mismatch and its consequences

    Hasan, Zubair


    This paper raises the issue of an initial structure-objective mismatch in the launching of Islamic finance. The abolition of interest and promotion of growth with equity were goals of the conceived system. These goals expressed a long run vision to improve the condition of the Muslim communities across the world. However, the organizational form adopted for Islamic finance was of the existing commercial banks which provided essentially short-term loans on interest to trade industry and commer...

  8. Islamic Finance:Sructure-objective mismatch and its consequences

    Hasan, Zubair


    This paper raises the issue of an initial structure-objective mismatch in the launching of Islamic finance. The abolition of interest and promotion of growth with equity were goals of the conceived system. These goals expressed a long run vision to improve the condition of the Muslim communities across the world. However, the organizational form adopted for Islamic finance was of the existing commercial banks which provided essentially short-term loans on interest to trade industry and commer...

  9. Phenotypic mismatches reveal escape from arms-race coevolution.

    Charles T Hanifin


    Full Text Available Because coevolution takes place across a broad scale of time and space, it is virtually impossible to understand its dynamics and trajectories by studying a single pair of interacting populations at one time. Comparing populations across a range of an interaction, especially for long-lived species, can provide insight into these features of coevolution by sampling across a diverse set of conditions and histories. We used measures of prey traits (tetrodotoxin toxicity in newts and predator traits (tetrodotoxin resistance of snakes to assess the degree of phenotypic mismatch across the range of their coevolutionary interaction. Geographic patterns of phenotypic exaggeration were similar in prey and predators, with most phenotypically elevated localities occurring along the central Oregon coast and central California. Contrary to expectations, however, these areas of elevated traits did not coincide with the most intense coevolutionary selection. Measures of functional trait mismatch revealed that over one-third of sampled localities were so mismatched that reciprocal selection could not occur given current trait distributions. Estimates of current locality-specific interaction selection gradients confirmed this interpretation. In every case of mismatch, predators were "ahead" of prey in the arms race; the converse escape of prey was never observed. The emergent pattern suggests a dynamic in which interacting species experience reciprocal selection that drives arms-race escalation of both prey and predator phenotypes at a subset of localities across the interaction. This coadaptation proceeds until the evolution of extreme phenotypes by predators, through genes of large effect, allows snakes to, at least temporarily, escape the arms race.

  10. Ballistic Thermal Conduction across Acoustically Mismatched Solid Junctions

    Jian WANG; Wang, Jian-Sheng


    We derive expressions for energy flow in terms of lattice normal mode coordinates and energy transmission involving reduced group velocities. With a version of Landauer formula appropriate for lattice dynamic approach, the phonon transmission coefficients and thermal conductance are calculated for two kinds of acoustically mismatched junctions: different chirality nanotubes (11,0) to (8,0), and Si-Ge superlattice structure. Our calculation shows a mode-dependent transmission in nanotube junct...

  11. Towards automatic identification of mismatched image pairs through loop constraints

    Elibol, Armagan; Kim, Jinwhan; Gracias, Nuno; Garcia, Rafael


    Obtaining image sequences has become easier and easier thanks to the rapid progress on optical sensors and robotic platforms. Processing of image sequences (e.g., mapping, 3D reconstruction, Simultaneous Localisation and Mapping (SLAM)) usually requires 2D image registration. Recently, image registration is accomplished by detecting salient points in two images and nextmatching their descriptors. To eliminate outliers and to compute a planar transformation (homography) between the coordinate frames of images, robust methods (such as Random Sample Consensus (RANSAC) and Least Median of Squares (LMedS)) are employed. However, image registration pipeline can sometimes provide sufficient number of inliers within the error bounds even when images do not overlap. Such mismatches occur especially when the scene has repetitive texture and shows structural similarity. In this study, we present a method to identify the mismatches using closed-loop (cycle) constraints. The method exploits the fact that images forming a cycle should have identity mapping when all the homographies between images in the cycle multiplied. Cycles appear when the camera revisits an area that was imaged before, which is a common practice especially for mapping purposes. Our proposal extracts several cycles to obtain error statistics for each matched image pair. Then, it searches for image pairs that have extreme error histogram comparing to the other pairs. We present experimental results with artificially added mismatched image pairs on real underwater image sequences.

  12. Infra-red parametric generation: Phase mismatch condition

    An analytical investigation is made for the Infrared parametric generation in doped semiconductor plasma under phase mismatch condition. Theoretical formulations are undertaken to determine induced polarization and threshold pump field for the onset of parametric generation in semiconductor plasma medium. The origin of this nonlinear interaction lies in the second order optical susceptibility arising due to the induced nonlinear current density in piezoelectric medium. Numerical estimations are made for n- type InSb at 77 K duly irradiated by a pulsed 10.6µm CO2 laser. It is very difficult to attain exact phase matching in experimental frame so we have considered a tolerable small phase mismatch in order to attain a new result. Its effect on the Infrared parametric generation in compound semiconductor is examined through induced polarization. Transmitted intensity is determined to have an idea about conversion efficiency of the said process. Phase mismatch tends to raise the required pump field to stimulate the parametric generation. Transmitted intensity is found to decrease with coherence length lc and increase carrier concentration n0, which is favorable for improved conversion efficiency

  13. Review: Clinical aspects of hereditary DNA Mismatch repair gene mutations.

    Sijmons, Rolf H; Hofstra, Robert M W


    Inherited mutations of the DNA Mismatch repair genes MLH1, MSH2, MSH6 and PMS2 can result in two hereditary tumor syndromes: the adult-onset autosomal dominant Lynch syndrome, previously referred to as Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the childhood-onset autosomal recessive Constitutional Mismatch Repair Deficiency syndrome. Both conditions are important to recognize clinically as their identification has direct consequences for clinical management and allows targeted preventive actions in mutation carriers. Lynch syndrome is one of the more common adult-onset hereditary tumor syndromes, with thousands of patients reported to date. Its tumor spectrum is well established and includes colorectal cancer, endometrial cancer and a range of other cancer types. However, surveillance for cancers other than colorectal cancer is still of uncertain value. Prophylactic surgery, especially for the uterus and its adnexa is an option in female mutation carriers. Chemoprevention of colorectal cancer with aspirin is actively being investigated in this syndrome and shows promising results. In contrast, the Constitutional Mismatch Repair Deficiency syndrome is rare, features a wide spectrum of childhood onset cancers, many of which are brain tumors with high mortality rates. Future studies are very much needed to improve the care for patients with this severe disorder. PMID:26746812

  14. Semiblind Hyperspectral Unmixing in the Presence of Spectral Library Mismatches

    Fu, Xiao; Ma, Wing-Kin; Bioucas-Dias, Jose M.; Chan, Tsung-Han


    The dictionary-aided sparse regression (SR) approach has recently emerged as a promising alternative to hyperspectral unmixing (HU) in remote sensing. By using an available spectral library as a dictionary, the SR approach identifies the underlying materials in a given hyperspectral image by selecting a small subset of spectral samples in the dictionary to represent the whole image. A drawback with the current SR developments is that an actual spectral signature in the scene is often assumed to have zero mismatch with its corresponding dictionary sample, and such an assumption is considered too ideal in practice. In this paper, we tackle the spectral signature mismatch problem by proposing a dictionary-adjusted nonconvex sparsity-encouraging regression (DANSER) framework. The main idea is to incorporate dictionary correcting variables in an SR formulation. A simple and low per-iteration complexity algorithm is tailor-designed for practical realization of DANSER. Using the same dictionary correcting idea, we also propose a robust subspace solution for dictionary pruning. Extensive simulations and real-data experiments show that the proposed method is effective in mitigating the undesirable spectral signature mismatch effects.

  15. Factory waste influence on Elbrus adjacent area

    Complete text of publication follows. Two major burials of waste from concentrating mill of The Tyrnauz Tungsten-Molybdic Plant are situated at the recreation area of Elbrus adjacent territory. But it is significant that the strong winds periods are available here at the river Baksan valley constantly, which are transfers the particles of burials along that valley. The waste pollutes pastures and agricultural lands. The sampling of the buried material on the surface and of the soil of adjacent territory was fulfilled here in order to check up that assumption. The chemical analyses of the buried waste were performed at the X-ray spectrometer Philips PW2400. The results received showed the high quantity of some environmental threat elements. The soil sample analyses on 20 elements (including Ca, Ti, Mn, Fe, P, S, Cr, V, Co, Ni, Cu, Zn, Sr, Zr, Ba, Pb, As, Mo, W, Sn) showed the excess of maximum permissible concentrations (according to Russian Federation hygienic regulations of some investigated elements in the all researched samples. Some results of the investigations are shown below in the table 1. The excesses of maximum permissible concentrations were observed for phosphorus from 5.3 to 11.3 times as much; for sulphur from 3 to 8.75 times as much; for cobalt from 2.6 to 4.6 times as much; for nickel from 8.25 to 15.25 times as much; for copper from 9.3 to 22 times as much; for arsenic up to 10 and at one sample - to 28 times as much. At the MPC level was the lead contents and also at single instances - the manganese and vanadium contents. The excess of background contents were observed for molybdenum up to 8, tungsten - up to 38 and tin - up to 7 times as much. Presented data confirmed the supposition that buried waste pollutes pastures and agricultural lands of Elbrus adjacent area and that means the necessity of either more careful burial of these waste products, or their full recycling.

  16. Seismicity in Azerbaijan and Adjacent Caspian Sea

    So far no general view on the geodynamic evolution of the Black Sea to the Caspian Sea region is elaborated. This is associated with the geological and structural complexities of the region revealed by geophysical, geochemical, petrologic, structural, and other studies. A clash of opinions on geodynamic conditions of the Caucasus region, sometimes mutually exclusive, can be explained by a simplified interpretation of the seismic data. In this paper I analyze available data on earthquake occurrences in Azerbaijan and the adjacent Caspian Sea region. The results of the analysis of macroseismic and instrumental data, seismic regime, and earthquake reoccurrence indicate that a level of seismicity in the region is moderate, and seismic event are concentrated in the shallow part of the lithosphere. Seismicity is mostly intra-plate, and spatial distribution of earthquake epicenters does not correlate with the plate boundaries

  17. Detecting Adjacent Relativity of Engineering Drawing Entities with Container Window

    林福严; 邱友申; 秦吉胜


    Automatic recognition and interpretation of engineering drawing plays an important role in computer aided engineering. Detecting the positional relation between entities is an important topic in this research field. In this paper the concepts of adjacent relativity and container window of drawing entities were proposed. By means of container window, the adjacent irrelative entities can be detected quickly and effectively, which speeds up the process of adjacent relativity detection. Meanwhile, the algorithm of adjacent relativity detection was discussed.

  18. Numerical simulations of material mismatch and ductile crack growth

    Oestby, Erling


    Both the global geometry and inhomogeneities in material properties will influence the fracture behaviour of structures in presence of cracks. In this thesis numerical simulations have been used to investigate how some aspects of both these issues affect the conditions at the crack-tip. The thesis is organised in an introduction chapter, summarising the major findings and conclusions, a review chapter, presenting the main aspects of the developments in the field of fracture mechanics, and three research papers. Paper I considers the effect of mismatch in hardening exponent on the local near-tip stress field for stationary interface cracks in bi-materials under small scale yielding conditions. It is demonstrated that the stress level in the weaker material increases compared to what is found in the homogeneous material for the same globally applied load level, with the effect being of increasing importance as the crack-tip is approached. Although a coupling between the radial and angular dependence of the stress fields exists, the evolving stress field can still be normalised with the applied J. The effect on the increase in stress level can closely be characterised by the difference in hardening exponent, {delta}n, termed the hardening mismatch, and is more or less independent of the absolute level of hardening in the two materials. Paper II and Ill deal with the effects of geometry, specimen size, hardening level and yield stress mismatch in relation to ductile crack growth. The ductile crack growth is simulated through use of the Gurson model. In Paper H the effect of specimen size on the crack growth resistance is investigated for deep cracked bend and shallow cracked tensile specimens. At small amounts of crack growth the effect of specimen size on the crack growth resistance is small, but a more significant effect is found for larger amounts of crack growth. The crack growth resistance decreases in smaller specimens loaded in tension, whereas the opposite is

  19. Construction of heteroduplex DNA and in vitro model for functional analysis of mismatch repair

    WANG Yi; Clark Alan; WANG Jiaxun; SUN Menghong; SHI Daren


    Functional deficiency of mismatch repair (MMR) system is one of the mechanisms of tumorigenesis. With the development of the investigation and the requirement from the clinical diagnosis and treatment it is necessary to build up a method to evaluate the functional status of the whole MMR system in the concerned tumors. The original ssDNA and dsDNA from wild type (wt) bacteriophage M13mp2 and its three derivates with mutation points in the lacZα Gene have been used to construct two kinds of heteroduplex DNA molecules. One named del(2) has two bases deleted in the negative strand, the other has a G·G mismatch base pair in the negative strand too. Introducing this heteroduplex DNA into E. Coli NR9162 (mutS-) without the MMR ability on the indicator plate with x-gal and IPTG, there are three kinds of plaques, mixture plaque as the characteristic phenotype of heteroduplex DNA, blue and clear plaques. If the cell extract is mismatch repair competent the percentage of the mixture plaque will decrease after incubation with these heteroduplex DNA, the repair efficiency is expressed in percentage as 100× (1 minus the ratio of percentages of mixture plaque obtained from the extract-treated sample and untreated samples), which can imply the functional status of MMR system of certain samples. After large T-antigen-dependent SV-40 DNA replication assay cell extract from TK6, a human lymphoblastoid B-cell lymphoma cell line with MMR ability, and Lovo, a human colonic carcinoma cell line with MMR deficiency have incubated with these heteroduplex DNA. The repair efficiency of TK6 to del(2) is more than 60%, to G·G is more than 50%. The Lovo efficiency to del(2) is less than 10%, to G·G is less than 20%. Therefore, in this in vitro model used for functional analysis of mismatch repair of heteroduplex DNA as the repair target, TK6 can serve as the control for MMR proficiency and Lovo as the control for MMR deficiency. Using this model the tumor tissue from a case of hereditary

  20. Study on Ground Motion Attenuation Relation in Shanghai and Its Adjacent Region

    Shi Shuzhong; Shen Jianwen


    Based on intensity data in Shanghai and its adjacent region, the intensity attenuation relation is determined. Selecting the western United States as a reference area where there are rich strong ground motion records and intensity data, and by determining ground motion attenuation relation in an area lacking in strong ground motion data, we obtain the ground motion attenuation relation in Shanghai and its adjacent region.

  1. Impact of adjacent land use on coastal wetland sediments.

    Karstens, Svenja; Buczko, Uwe; Jurasinski, Gerald; Peticzka, Robert; Glatzel, Stephan


    Coastal wetlands link terrestrial with marine ecosystems and are influenced from both land and sea. Therefore, they are ecotones with strong biogeochemical gradients. We analyzed sediment characteristics including macronutrients (C, N, P, K, Mg, Ca, S) and heavy metals (Mn, Fe, Cu, Zn, Al, Co, Cr, Ni) of two coastal wetlands dominated by Phragmites australis at the Darss-Zingst Bodden Chain, a lagoon system at the Southern Baltic Sea, to identify the impact of adjacent land use and to distinguish between influences from land or sea. In the wetland directly adjacent to cropland (study site Dabitz) heavy metal concentrations were significantly elevated. Fertilizer application led to heavy metal accumulation in the sediments of the adjacent wetland zones. In contrast, at the other study site (Michaelsdorf), where the hinterland has been used as pasture, heavy metal concentrations were low. While the amount of macronutrients was also influenced by vegetation characteristics (e.g. carbon) or water chemistry (e.g. sulfate), the accumulation of heavy metals is regarded as purely anthropogenic influence. A principal component analysis (PCA) based on the sediment data showed that the wetland fringes of the two study sites are not distinguishable, neither in their macronutrient status nor in their concentrations of heavy metals, whereas the interior zones exhibit large differences in terms of heavy metal concentrations. This suggests that seaside influences are minor compared to influences from land. Altogether, heavy metal concentrations were still below national precautionary and action values. However, if we regard the macronutrient and heavy metal concentrations in the wetland fringes as the natural background values, an accumulation of trace elements from agricultural production in the hinterland is apparent. Thus, coastal wetlands bordering croplands may function as effective pollutant buffers today, but the future development has to be monitored closely to avoid

  2. Effect of strength mismatch on fracture mechanical behavior of NG-DMW

    In modern pressurized water reactor (PWR) designs, dissimilar metal joints, e.g. reactor pressure vessel (RPV) safe-ends, are manufactured using a new weld design which takes advantage of narrow-gap (NG) welding technique. In addition to the new weld design, the filler metals have been changed from Alloys 82 and 182 to higher Cr containing Alloys 52 and 152 to ensure the structural integrity of the welds. In dissimilar metal welds (DMW), the mismatch in material properties between the two joined materials and their narrow local variation in different zones of the weld are of importance because the local strength mismatch state plays an important role in the fracture behavior of the weld. For the experimental determination of the local strength variations in a narrow-gap dissimilar metal weld (NG-DMW), a weld mock-up was manufactured using narrow-gap gas-tungsten arc welding (GTAW) method. The weld consisted of SA 508 pressure vessel steel with AISI 309L/308L cladding, AISI 304 piping steel, and Alloy 52 weld metal. The weld was characterized in two different heat treatment conditions, in as-welded condition and in post-weld heat treated (PWHT) condition. The microstructure of the weld mock-up was characterized using FEGSEM. The fusion zone (FZ) between the SA 508 pressure vessel steel and Alloy 52 weld metal was characterized using micro- and nano-hardness testing and the strength mismatch state of the FZ was determined with tensile testing using miniature-sized tensile testing specimens allowing the determination of the local tensile properties of the narrow weld zones near the fusion line (FL). The fracture mechanical testing was performed at room temperature to examine the effect of local strength mismatch on the fracture behavior and crack propagation. The results of the tensile tests revealed that Alloy 52 weld metal had close to equivalent strength with SA 508 base material and the highest strength mismatch existed between the SA 508 heat-affected zone (HAZ

  3. Genome instability of mis-match repair and its role in carcinogenesis due to radiation

    Homologue genes, mutS and mutL have been known as a key gene for mismatch repair in E. coli. In this study, identification of such homologue genes in the nematode was attempted using caenorhabditis elegans and three kinds of mutS homologues (mshG, mshZ and mshF) and 2 kinds of mutL ones were identified. From after isolation of these genes, base sequences were analyzed. Then, an insertion mutant in which Tcl transposon is inserted in Exon 13 positioned at the center of mshF was screened and its homozygote where breakage of transposon in somatic cells occurred frequently was obtained and its morphological changes were not significant. In the nematoda, we detected a highly conserved domain in mutS family gene, which is commonly present in yeast and human genes. Based on the amino acid sequence of this domain, four kinds of primers were constructed for PCR reaction using the whole DNA from the nematoda as a template and four DNA fragments of which sizes were almost corresponding to the homologue proteins were produced. From screening of Tc1 insertion mutant for 8 mismatch repair genes, three strains; mshF, rqhW and RqhY were obtained and the gene structures and the positions of Tc1 insertion in these strains were determined. The sensitivities to ionizing radiation, UV and alkyl reagent of these strains were not significantly different from those of the wild strain. (M.N.)

  4. Mismatch recognition-coupled stabilization of Msh2-Msh6 in an ATP-bound state at the initiation of DNA repair.

    Antony, Edwin; Hingorani, Manju M


    Mismatch repair proteins correct errors in DNA via an ATP-driven process. In eukaryotes, the Msh2-Msh6 complex recognizes base pair mismatches and small insertion/deletions in DNA and initiates repair. Both Msh2 and Msh6 proteins contain Walker ATP-binding motifs that are necessary for repair activity. To understand how these proteins couple ATP binding and hydrolysis to DNA binding/mismatch recognition, the ATPase activity of Saccharomyces cerevisiae Msh2-Msh6 was examined under pre-steady-state conditions. Acid-quench experiments revealed that in the absence of DNA, Msh2-Msh6 hydrolyzes ATP rapidly (burst rate = 3 s(-1) at 20 degrees C) and then undergoes a slow step in the pathway that limits catalytic turnover (k(cat) = 0.1 s(-1)). ATP is hydrolyzed similarly in the presence of fully matched duplex DNA; however, in the presence of a G:T mismatch or +T insertion-containing DNA, ATP hydrolysis is severely suppressed (rate = 0.1 s(-1)). Pulse-chase experiments revealed that Msh2-Msh6 binds ATP rapidly in the absence or in the presence of DNA (rate = 0.1 microM(-1) s(-1)), indicating that for the Msh2-Msh6.mismatched DNA complex, a step after ATP binding but before or at ATP hydrolysis is the rate-limiting step in the pathway. Thus, mismatch recognition is coupled to a dramatic increase in the residence time of ATP on Msh2-Msh6. This mismatch-induced, stable ATP-bound state of Msh2-Msh6 likely signals downstream events in the repair pathway. PMID:12820877

  5. Adaptive Neural Stabilizing Controller for a Class of Mismatched Uncertain Nonlinear Systems by State and Output Feedback.

    Arefi, Mohammad Mehdi; Jahed-Motlagh, Mohammad Reza; Karimi, Hamid Reza


    In this paper, first, an adaptive neural network (NN) state-feedback controller for a class of nonlinear systems with mismatched uncertainties is proposed. By using a radial basis function NN (RBFNN), a bound of unknown nonlinear functions is approximated so that no information about the upper bound of mismatched uncertainties is required. Then, an observer-based adaptive controller based on RBFNN is designed to stabilize uncertain nonlinear systems with immeasurable states. The state-feedback and observer-based controllers are based on Lyapunov and strictly positive real-Lyapunov stability theory, respectively, and it is shown that the asymptotic convergence of the closed-loop system to zero is achieved while maintaining bounded states at the same time. The presented methods are more general than the previous approaches, handling systems with no restriction on the dimension of the system and the number of inputs. Simulation results confirm the effectiveness of the proposed methods in the stabilization of mismatched nonlinear systems. PMID:25265641

  6. Stress Wave Interaction Between Two Adjacent Blast Holes

    Yi, Changping; Johansson, Daniel; Nyberg, Ulf; Beyglou, Ali


    Rock fragmentation by blasting is determined by the level and state of stress in the rock mass subjected to blasting. With the application of electronic detonators, some researchers stated that it is possible to achieve improved fragmentation through stress wave superposition with very short delay times. This hypothesis was studied through theoretical analysis in the paper. First, the stress in rock mass induced by a single-hole shot was analyzed with the assumptions of infinite velocity of detonation and infinite charge length. Based on the stress analysis of a single-hole shot, the stress history and tensile stress distribution between two adjacent holes were presented for cases of simultaneous initiation and 1 ms delayed initiation via stress superposition. The results indicated that the stress wave interaction is local around the collision point. Then, the tensile stress distribution at the extended line of two adjacent blast holes was analyzed for a case of 2 ms delay. The analytical results showed that the tensile stress on the extended line increases due to the stress wave superposition under the assumption that the influence of neighboring blast hole on the stress wave propagation can be neglected. However, the numerical results indicated that this assumption is unreasonable and yields contrary results. The feasibility of improving fragmentation via stress wave interaction with precise initiation was also discussed. The analysis in this paper does not support that the interaction of stress waves improves the fragmentation.

  7. Soil Structure Interaction between Two Adjacent Buildings under Earthquake Load

    Mahmoud Yahyai


    Full Text Available In some cases, tall buildings are located in geotechnically unsuitable places, due to their high ratio of height to width; there is risk of uplift and other effects such as overturning and reduction structure serviceability during earthquake. This research is aimed to evaluate the effect of Soil-Structure Interaction (SSI on seismic behavior of two adjacent 32 story buildings such as time period, base shear and displacements. The interaction effects are investigated for variable distance between the two buildings. Three types of soil such as soft clay, sandy gravel and compacted sandy gravel are considered for this study. The result obtained that the interaction effect increases time period of both buildings base shear and lateral displacement of the structures increases.


    Cevdet MERİÇ


    Full Text Available In this study, the jointing process of the SAE 1020 low carbon steel, generally used in the industry, has been done by the MAG weld method. The aim of this study is to examine the mismatch between base and weld metal. After the jointing process, mechanical and metalographical properties of the weld region, HAZ, and the weld metal of the samples considered here were searched, and CTOD (Crack Tip Opening Displacement was identified.

  9. Review of current topics on power cooling mismatch experiments

    A review is made of the current studies of fuel behavior under Power-Cooling-Mismatch (PCM) conditions as part of the light water reactor safety research program. Requirements of research in the field, both in-pile and out-of-pile, are discussed on the basis of the review including the present capability of fuel behavior analysis during a PCM accident, power reactor safety criteria, and experimental results available. Experimental plans in Japan and other countries of PCM research are also described. (auth.)

  10. Triple helix structures: sequence dependence, flexibility and mismatch effects.

    Sun, J S; Mergny, J L; Lavery, R; Montenay-Garestier, T; Hélène, C


    By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine. homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen-bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption. PMID:1815635