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Sample records for adjacent mismatch base

  1. Heterodyne detection with mismatch correction base on array detector

    Hongzhou, Dong; Guoqiang, Li; Ruofu, Yang; Chunping, Yang; Mingwu, Ao

    2016-07-01

    Based on an array detector, a new heterodyne detection system, which can correct the mismatches of amplitude and phase between signal and local oscillation (LO) beams, is presented in this paper. In the light of the fact that, for a heterodyne signal, there is a certain phase difference between the adjacent two samples of analog-to-digital converter (ADC), we propose to correct the spatial phase mismatch by use of the time-domain phase difference. The corrections can be realized by shifting the output sequences acquired from the detector elements in the array, and the steps of the shifting depend on the quantity of spatial phase mismatch. Numerical calculations of heterodyne efficiency are conducted to confirm the excellent performance of our system. Being different from previous works, our system needs not extra optical devices, so it provides probably an effective means to ease the problem resulted from the mismatches.

  2. Heterodyne detection with mismatch correction based on array detector

    Dong, Hongzhou; Li, Guoqiang; Yang, Ruofu; Yang, Chunping; Ao, Mingwu

    2016-07-01

    Based on an array detector, a new heterodyne detection system, which can correct the mismatches of amplitude and phase between signal and local oscillation (LO) beams, is presented in this paper. In the light of the fact that, for a heterodyne signal, there is a certain phase difference between the adjacent two samples of analog-to-digital converter (ADC), we propose to correct the spatial phase mismatch by use of the time-domain phase difference. The corrections can be realized by shifting the output sequences acquired from the detector elements in the array, and the steps of the shifting depend on the quantity of spatial phase mismatch. Numerical calculations of heterodyne efficiency are conducted to confirm the excellent performance of our system. Being different from previous works, our system needs not extra optical devices, so it provides probably an effective means to ease the problem resulted from the mismatches.

  3. The effect of base pair mismatch on DNA strand displacement

    Broadwater, Bo

    2016-01-01

    DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized. Here, we measured toehold-mediated strand displacement kinetics using single-molecule fluorescence in the presence of a single base pair mismatch. The apparent displacement rate varied significantly when the mismatch was introduced in the invading DNA strand. The rate generally decreased as the mismatch in the invader was encountered earlier in displacement. Our data indicate that a single base pair mismatch in the invader stalls branch migration, and displacement occurs via direct dissociation of the destabilized incumbent strand from the substrate strand. We combined both branch migration and direct dissociation into a model, which we term, the concurrent displacement model, and used the first passage t...

  4. Single-base mismatch detection based on charge transduction through DNA.

    Kelley, S O; Boon, E M; Barton, J K; Jackson, N M; Hill, M. G.

    1999-01-01

    High-throughput DNA sensors capable of detecting single-base mismatches are required for the routine screening of genetic mutations and disease. A new strategy for the electrochemical detection of single-base mismatches in DNA has been developed based upon charge transport through DNA films. Double-helical DNA films on gold surfaces have been prepared and used to detect DNA mismatches electrochemically. The signals obtained from redox-active intercalators bound to DNA-modified gold surfaces d...

  5. Reactivity of potassium permanganate and tetraethylammonium chloride with mismatched bases and a simple mutation detection protocol.

    Lambrinakos, A; Humphrey, K E; Babon, J J; Ellis, T P; Cotton, R G

    1999-01-01

    Many mutation detection techniques rely upon recognition of mismatched base pairs in DNA hetero-duplexes. Potassium permanganate in combination with tetraethylammonium chloride (TEAC) is capable of chemically modifying mismatched thymidine residues. The DNA strand can then be cleaved at that point by treatment with piperidine. The reactivity of potassium permanganate (KMnO4) in TEAC toward mismatches was investigated in 29 different mutations, representing 58 mismatched base pairs and 116 mis...

  6. Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA

    Cristovao, M.; Sisamakis, E.; Hingorani, M M; Marx, A. D.; Jung, C. P.; Rothwell, P. J.; Seidel, C. A. M.; Friedhoff, P.

    2012-01-01

    Mismatch repair (MMR) corrects replication errors such as mismatched bases and loops in DNA. The evolutionarily conserved dimeric MMR protein MutS recognizes mismatches by stacking a phenylalanine of one subunit against one base of the mismatched pair. In all crystal structures of G:T mismatch-bound MutS, phenylalanine is stacked against thymine. To explore whether these structures reflect directional mismatch recognition by MutS, we monitored the orientation of Escherichia coli MutS binding ...

  7. Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA

    M. Cristovao (Michele); E. Sisamakis (Evangelos); M.M. Hingorani (Manju); A.D. Marx (Andreas); C.P. Jung (Caroline); P.J. Rothwell (Paul); C.A.M. Seidel (Claus A.); P. Friedhoff (Peter)

    2012-01-01

    textabstractMismatch repair (MMR) corrects replication errors such as mismatched bases and loops in DNA. The evolutionarily conserved dimeric MMR protein MutS recognizes mismatches by stacking a phenylalanine of one subunit against one base of the mismatched pair. In all crystal structures of G:T mi

  8. Model Mismatch Paradigm for Probe based Nanoscale Imaging

    Agarwal, Pranav

    Scanning Probe Microscopes (SPMs) are widely used for investigation of material properties and manipulation of matter at the nanoscale. These instruments are considered critical enablers of nanotechnology by providing the only technique for direct observation of dynamics at the nanoscale and affecting it with sub Angstrom resolution. Current SPMs are limited by low throughput and lack of quantitative measurements of material properties. Various applications like the high density data storage, sub-20 nm lithography, fault detection and functional probing of semiconductor circuits, direct observation of dynamical processes involved in biological samples viz. motor proteins and transport phenomena in various materials demand high throughput operation. Researchers involved in material characterization at nanoscale are interested in getting quantitative measurements of stiffness and dissipative properties of various materials in a least invasive manner. In this thesis, system theoretic concepts are used to address these limitations. The central tenet of the thesis is to model, the known information about the system and then focus on perturbations of these known dynamics or model, to sense the effects due to changes in the environment such as changes in material properties or surface topography. Thus a model mismatch paradigm for probe based nanoscale imaging is developed. The topic is developed by presenting physics based modeling of a particular mode of operation of SPMs called the dynamic mode operation. This mode is modeled as a forced Lure system where a linear time invariant system is in feedback with an unknown static memoryless nonlinearity. Tools from averaging theory are used to tame this complex nonlinear system by approximating it as a linear system with time varying parameters. Material properties are thus transformed from being parameters of unknown nonlinear functions to being unknown coefficients of a linear plant. The first contribution of this thesis

  9. A polymerization-based method to construct a plasmid containing clustered DNA damage and a mismatch.

    Takahashi, Momoko; Akamatsu, Ken; Shikazono, Naoya

    2016-10-01

    Exposure of biological materials to ionizing radiation often induces clustered DNA damage. The mutagenicity of clustered DNA damage can be analyzed with plasmids carrying a clustered DNA damage site, in which the strand bias of a replicating plasmid (i.e., the degree to which each of the two strands of the plasmid are used as the template for replication of the plasmid) can help to clarify how clustered DNA damage enhances the mutagenic potential of comprising lesions. Placement of a mismatch near a clustered DNA damage site can help to determine the strand bias, but present plasmid-based methods do not allow insertion of a mismatch at a given site in the plasmid. Here, we describe a polymerization-based method for constructing a plasmid containing clustered DNA lesions and a mismatch. The presence of a DNA lesion and a mismatch in the plasmid was verified by enzymatic treatment and by determining the relative abundance of the progeny plasmids derived from each of the two strands of the plasmid. PMID:27449134

  10. MEASURES OF OCCUPATIONAL MISMATCH

    Monica Mihaela MAER MATEI

    2014-11-01

    Full Text Available The research developed in this paper is based on micro data available in Programme for the International Assessment of Adult Competencies (PIAAC. The research aimed to estimate the size of both forms of labour market mismatch: education mismatch and skill mismatch. The first measure of job mismatch is based on workers’ self-assessment. The second one uses the PIAAC assessment regarding the proficiency for each skill dimension (literacy, numeracy and problem solving in technology rich environments. The labor market mismatch was measured for Spain and Italy datasets for the higher education graduates whose occupations are included in Major Group two Professionals, according to the International Standard Classification of Occupations. The estimation results showed that the two measures of labour market mismatch are not correlated.

  11. Performance Analysis of Spectral Amplitude Coding Based OCDMA System with Gain and Splitter Mismatch

    Umrani, Fahim A.; Umrani, A. Waheed; Umrani, Naveed A.; Memon, Kehkashan A.; Kalwar, Imtiaz Hussain

    2013-09-01

    This paper presents the practical analysis of the optical code-division multiple-access (O-CDMA) systems based on perfect difference codes. The work carried out use SNR criterion to select the optimal value of avalanche photodiodes (APD) gain and shows how the mismatch in the splitters and gains of the APD used in the transmitters and receivers of network can degrade the BER performance of the system. The investigations also reveal that higher APD gains are not suitable for such systems even at higher powers. The system performance, with consideration of shot noise, thermal noise, bulk and surface leakage currents is also investigated.

  12. Effect of base mismatch on the electronic properties of DNA-DNA and LNA-DNA double strands: Density-functional theoretical calculations

    Natsume, Takayuki; Ishikawa, Yasuyuki; Dedachi, Kenichi; Tsukamoto, Takayuki; Kurita, Noriyuki

    2007-01-01

    The electronic properties of double-stranded octametric DNA-DNA and LNA-DNA with a single-base mismatch were compared with those having fully complementary base pairs to quantify the effect of the base mismatch on hybridization energies (HE). A single T-G mismatch in the LNA-DNA gives rise to a significant reduction in HE, which is consistent with a significant lowering of the melting temperature for mismatched LNA-DNA. By contrast, the hybridization strength of the mismatched DNA-DNA depends...

  13. Compensating Acoustic Mismatch Using Class-Based Histogram Equalization for Robust Speech Recognition

    Suh, Youngjoo; Kim, Sungtak; Kim, Hoirin

    2007-12-01

    A new class-based histogram equalization method is proposed for robust speech recognition. The proposed method aims at not only compensating for an acoustic mismatch between training and test environments but also reducing the two fundamental limitations of the conventional histogram equalization method, the discrepancy between the phonetic distributions of training and test speech data, and the nonmonotonic transformation caused by the acoustic mismatch. The algorithm employs multiple class-specific reference and test cumulative distribution functions, classifies noisy test features into their corresponding classes, and equalizes the features by using their corresponding class reference and test distributions. The minimum mean-square error log-spectral amplitude (MMSE-LSA)-based speech enhancement is added just prior to the baseline feature extraction to reduce the corruption by additive noise. The experiments on the Aurora2 database proved the effectiveness of the proposed method by reducing relative errors by[InlineEquation not available: see fulltext.] over the mel-cepstral-based features and by[InlineEquation not available: see fulltext.] over the conventional histogram equalization method, respectively.

  14. Blind Decorrelating Detection Based on Particle Swarm Optimization under Spreading Code Mismatch

    Jhih-Chung Chang; Chih-Chang Shen

    2014-01-01

    A way of resolving spreading code mismatches in blind multiuser detection with a particle swarm optimization (PSO) approach is proposed. It has been shown that the PSO algorithm incorporating the linear system of the decorrelating detector, which is termed as decorrelating PSO (DPSO), can significantly improve the bit error rate (BER) and the system capacity. As the code mismatch occurs, the output BER performance is vulnerable to degradation for DPSO. With a blind decorrelating scheme, the proposed blind DPSO (BDPSO) offers more robust capabilities over existing DPSO under code mismatch scenarios.

  15. Low frequency of defective mismatch repair in a population-based series of upper urothelial carcinoma

    Isfoss Björn L

    2005-03-01

    Full Text Available Abstract Background Upper urothelial cancer (UUC, i.e. transitional cell carcinomas of the renal pelvis and the ureter, occur at an increased frequency in patients with hereditary nonpolyposis colorectal cancer (HNPCC. Defective mismatch repair (MMR specifically characterizes HNPCC-associated tumors, but also occurs in subsets of some sporadic tumors, e.g. in gastrointestinal cancer and endometrial cancer. Methods We assessed the contribution of defective MMR to the development of UUC in a population-based series from the southern Swedish Cancer Registry, through microsatellite instability (MSI analysis and immunohistochemical evaluation of expression of the MMR proteins MLH1, PMS2, MSH2, and MSH6. Results A MSI-high phenotype was identified in 9/216 (4% successfully analyzed patients and a MSI-low phenotype in 5/216 (2%. Loss of MMR protein immunostaining was found in 11/216 (5% tumors, and affected most commonly MSH2 and MSH6. Conclusion This population-based series indicates that somatic MMR inactivation is a minor pathway in the development of UUC, but tumors that display defective MMR are, based on the immunohistochemical expression pattern, likely to be associated with HNPCC.

  16. Non-target adjacent stimuli classification improves performance of classical ERP-based brain computer interface

    Ceballos, G. A.; Hernández, L. F.

    2015-04-01

    Objective. The classical ERP-based speller, or P300 Speller, is one of the most commonly used paradigms in the field of Brain Computer Interfaces (BCI). Several alterations to the visual stimuli presentation system have been developed to avoid unfavorable effects elicited by adjacent stimuli. However, there has been little, if any, regard to useful information contained in responses to adjacent stimuli about spatial location of target symbols. This paper aims to demonstrate that combining the classification of non-target adjacent stimuli with standard classification (target versus non-target) significantly improves classical ERP-based speller efficiency. Approach. Four SWLDA classifiers were trained and combined with the standard classifier: the lower row, upper row, right column and left column classifiers. This new feature extraction procedure and the classification method were carried out on three open databases: the UAM P300 database (Universidad Autonoma Metropolitana, Mexico), BCI competition II (dataset IIb) and BCI competition III (dataset II). Main results. The inclusion of the classification of non-target adjacent stimuli improves target classification in the classical row/column paradigm. A gain in mean single trial classification of 9.6% and an overall improvement of 25% in simulated spelling speed was achieved. Significance. We have provided further evidence that the ERPs produced by adjacent stimuli present discriminable features, which could provide additional information about the spatial location of intended symbols. This work promotes the searching of information on the peripheral stimulation responses to improve the performance of emerging visual ERP-based spellers.

  17. A method of 3D CAD model retrieval based on feature adjacent graph

    Shi, Min; Zhang, Shusheng

    2013-03-01

    To reuse the 3D CAD model, a new method of 3D CAD model retrieval based on feature adjacent graph is proposed. First, a representation of feature adjacent graph for the CAD model is extracted by automatic identification-based and interactive identification-assisted. Next, the vertex product graph of the two CAD model's feature adjacent graphs is constructed, and then the ant colony algorithm is employed to detect the maximum-cliques in the vertex product graph. At last, the similarity of the models is calculated by comparing the maximum-cliques. Experimental results show that this method can realize the 3D CAD model retrieval and its efficiency meets the requirements of applications.

  18. The γ/γ ' mismatch in Ni based superalloys: In situ measurements during a creep test

    Diologent, F.; Caron, P.; d'Almeida, T.; Jacques, A.; Bastie, P.

    2003-01-01

    The lattice mismatch between the fcc γ matrix and the ordered γ ' cuboı̈dal precipitates in superalloys induces large internal stresses within the material. These stresses have a major effect on its mechanical behaviour and on the anisotropic evolution of the microstructure (rafting…) during its lifetime. The evolution of the effective lattice mismatch of the AM1 and MCNG superalloys was measured continuously during high temperature creep tests (1100 °C, 150 MPa) at the ID 15 (high energy) beamline of the ESRF. The bulk profiles of the 200 reflection (parallel to the tensile axis) were recorded using the triple crystal diffractometer. Both materials have a negative mismatch, and exhibit a transition between the initial wide γ ' peak to a two peaks profile during stage I of the creep curve, as rafting takes place. During stage II, the 200 mismatch decreases in magnitude. During stage III, as a microstructural transition (coalescence) occurs and the strain rate increases, the mismatch changes in the same direction, but at a faster rate, while the thickness of both peaks increases. Evolution of the MCNG specimen was the same as AM1 ones, but rafting and transition to stage II take place at a different rate.

  19. Label-free detection of DNA single-base mismatches using a simple reflectance-based optical technique.

    Nava, G; Ceccarello, E; Giavazzi, F; Salina, M; Damin, F; Chiari, M; Buscaglia, M; Bellini, T; Zanchetta, G

    2016-05-21

    Rapid and quantitative detection of the binding of nucleic acids to surface-immobilized probes remains a challenge in many biomedical applications. We investigated the hybridization of a set of fully complementary and defected 12-base long DNA oligomers by using the Reflective Phantom Interface (RPI), a recently developed multiplexed label-free detection technique. Based on the simple measurement of reflected light intensity, this technology enables to quantify the hybridization directly as it occurs on the surface with a sensitivity of 10 pg mm(-2). We found a strong effect of single-base mismatches and of their location on hybridization kinetics and equilibrium binding. In line with previous studies, we found that DNA-DNA binding is weaker on a surface than in the bulk. Our data indicate that this effect is a consequence of weak nonspecific binding of the probes to the surface. PMID:27122358

  20. Gold-based optical biosensor for single-mismatched DNA detection using salt-induced hybridization

    Zhan, Zongrui; Ma, Xingyi; Cao, Cuong;

    2011-01-01

    DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the...... presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which...

  1. Magnetostatic-Wave-Based Magneto-Optic Pulse Compression by Control of Phase Mismatching

    WU Bao-Jian; GAO Xiang

    2008-01-01

    Microwave magnetostatic waves (MSWs) as moving gratings in magneto-optic (MO) film can lead to the Bragg diffraction of guided optical waves (GOWs). The MO coupling characteristics are responsible for the amplitude and phase frequency spectra of diffracted pulses and even result in the compression of chirped optical pulses in time domain. We theoretically investigate the noncollinear diffraction of linearly chirped Gaussian optical pulses by continuous magnetostatic forward volume waves in detail. For a given chirped optical pulse, with the increase of phase-mismatching slopes, the compression efficiency (CE) is gradually improved up to the maximum followed by the transition of diffracted pulses from single peak to multi peaks. The larger the chirp parameter is,the smaller the required phase-mismatching slope to achieve the maximal CE is. However, the rise of the chirp parameter or phase-mismatching slope reduces the relative peak intensity of the diffracted pulse. Lastly, it is pointed out that the phase-mismatching slope can be greatly increased by using the high-order modes of MSWs and GOWs.

  2. Statistical weighting of model-based optoacoustic reconstruction for minimizing artefacts caused by strong acoustic mismatch

    Deán-Ben, X. Luís; Razansky, Daniel; Ntziachristos, Vasilis

    2011-03-01

    A modified quantitative inversion algorithm is presented that minimizes the effects of internal acoustic reflections or scattering in tomographic optoacoustic images. The inversion procedure in our model-based algorithm consists in solving a linear system of equations in which each individual equation corresponds to a given position of the acoustic transducer and to a given time instant. Thus, the modification that we propose in this work consists in weighting each equation of the linear system with the probability that the measured wave is not distorted by reflection or scattering phenomena. We show that the probability that a reflected or scattered wave is detected at a given position and at a given instant is approximately proportional to the size of the area in which the original wave could have been generated, which is dependent on the position of the transducer and on the time instant, so that such probability can be used to weight each equation of the linear system. Thereby, the contribution of the waves that propagate directly to the transducer to the reconstructed images is emphasized. We experimentally test the proposed inversion algorithm with tissue-mimicking agar phantoms in which air-gaps are included to cause reflections of the acoustic waves. The tomographic reconstructions obtained with the modification proposed herein show a clear reduction of the artefacts due to these acoustic phenomena with respect to the reconstructions yielded with the original algorithm. This performance is directly related to in-vivo small animal imaging applications involving imaging in the presence of bones, lungs, and other highly mismatched organs.

  3. The Escherichia coli Methyl-Directed Mismatch Repair System Repairs Base Pairs Containing Oxidative Lesions

    Wyrzykowski, Jennifer; Volkert, Michael R.

    2003-01-01

    A major role of the methyl-directed mismatch repair (MMR) system of Escherichia coli is to repair postreplicative errors. In this report, we provide evidence that MMR also acts on oxidized DNA, preventing mutagenesis. When cells deficient in MMR are grown anaerobically, spontaneous mutation frequencies are reduced compared with those of the same cells grown aerobically. In addition, we show that a dam mutant has an increased sensitivity to hydrogen peroxide treatment that can be suppressed by...

  4. Analysis of the effects of mismatched errors on coherent beam combining based on a self-imaging waveguide

    Tao, R.; Wang, X.; Zhou, Pu; Si, Lei

    2016-01-01

    A theoretical model of coherent beam combining (CBC) based on a self-imaging waveguide (SIW) is built and the effects of mismatched errors on SIW-based CBC are simulated and analysed numerically. With the combination of the theoretical model and the finite difference beam propagation method, two main categories of errors, assembly and nonassembly errors, are numerically studied to investigate their effect on the beam quality by using the M2 factor. The optimisation of the SIW and error control principle of the system is briefly discussed. The generalised methodology offers a good reference for investigating waveguide-based high-power coherent combining of fibre lasers in a comprehensive way.

  5. Estimation of channel mismatches in time-interleaved analog-to-digital converters based on fractional delay and sine curve fitting.

    Guo, Lianping; Tian, Shulin; Jiang, Jun

    2015-03-01

    This paper proposes an algorithm to estimate the channel mismatches in time-interleaved analog-to-digital converter (TIADC) based on fractional delay (FD) and sine curve fitting. Choose one channel as the reference channel and apply FD to the output samples of reference channel to obtain the ideal samples of non-reference channels with no mismatches. Based on least square method, the sine curves are adopted to fit the ideal and the actual samples of non-reference channels, and then the mismatch parameters can be estimated by comparing the ideal sine curves and the actual ones. The principle of this algorithm is simple and easily understood. Moreover, its implementation needs no extra circuits, lowering the hardware cost. Simulation results show that the estimation accuracy of this algorithm can be controlled within 2%. Finally, the practicability of this algorithm is verified by the measurement results of channel mismatch errors of a two-channel TIADC prototype. PMID:25832264

  6. Strategy in clinical practice for classification of unselected colorectal tumours based on mismatch repair deficiency

    Jensen, Lars Henrik; Lindebjerg, J; Byriel, L;

    2007-01-01

    Objective Deficiency of DNA mismatch repair (MMR) causes microsatellite instability (MSI) in a subset of colorectal cancers. Patients with these tumours have a better prognosis and may have an altered response to chemotherapy. Some of the tumours are caused by hereditary mutations (hereditary non...... methylation or BRAF mutation analysis to distinguish sporadic patients from likely hereditary ones. MMR deficient patients with sporadic disease can be reassured of the better prognosis and the likely hereditary cases should receive genetic counselling.......Objective Deficiency of DNA mismatch repair (MMR) causes microsatellite instability (MSI) in a subset of colorectal cancers. Patients with these tumours have a better prognosis and may have an altered response to chemotherapy. Some of the tumours are caused by hereditary mutations (hereditary...... nonpolyposis colon cancer or Lynch syndrome), but most are epigenetic changes of sporadic origin. The aim of this study was to define a robust and inexpensive strategy for such classification in clinical practice. Method Tumours and blood samples from 262 successive patients with colorectal adenocarcinomas...

  7. A Layered Zone Routing Algorithm in Ad Hoc Network Based on Matrix of Adjacency Connection

    XU Guang-wei; LI Feng; SHI Xiu-jin; HUO Jia-zhen

    2007-01-01

    The hybrid routing protocol has received more attention recently than the proactive and the reactive, especially for large-scale and highly dynamic connection, in mobile ad hoc network. A crucial reason is that zone-layered is being utilized in the complex systems. A hybrid routing algorithm which is layered zone based on adjacency connection(LZBAC) is put forward under the background of a few members in network with steady position and link. The algorithm modifies storage structure of nodes and improves routing mechanism. The theoretical analysis and simulation testing testify that the algorithm costs shorter time of route finding and less delay than others.

  8. Adjacency Matrix Based Energy Efficient Scheduling Using S-Mac Protocol in Wireless Sensor Networks

    Ravindara Bhatt,

    2012-04-01

    Full Text Available Communication is the main motive in any Networks whether it is Wireless Sensor Network, Ad-Hoc networks, Mobile Networks, Wired Networks, Local Area Network, Metropolitan Area Network, Wireless Area Network etc, hence it must be energy efficient. The main parameters for energy efficient communication are maximizing network lifetime, saving energy at the different nodes, sending the packets in minimum time delay, higher throughput etc. This paper focuses mainly on the energy efficient communication with the help of Adjacency Matrix in the Wireless Sensor Networks. The energy efficient scheduling can be done by putting the idle node in to sleep node so energy at the idle node can be saved. The proposed model in this paper first forms the adjacency matrix and broadcasts the information aboutthe total number of existing nodes with depths to the other nodes in the same cluster from controller node. When every node receives the node information about the other nodes for same cluster they communicate based on the shortest depths and schedules the idle node in to sleep mode for a specific time threshold so energy at the idle nodes can be saved.

  9. Effect of LNA- and OMeN-modified oligonucleotide probes on the stability and discrimination of mismatched base pairs of duplexes

    Ying Yan; Jing Yan; Xianyu Piao; Tianbiao Zhang; Yifu Guan

    2012-06-01

    Locked nucleic acid (LNA) and 2′--methyl nucleotide (OMeN) are the most extensively studied nucleotide analogues. Although both LNA and OMeN are characterized by the C3′-endo sugar pucker conformation, which is dominant in A-form DNA and RNA nucleotides, they demonstrate different binding behaviours. Previous studies have focused attention on their properties of duplex stabilities, hybridization kinetics and resistance against nuclease digestion; however, their ability to discriminate mismatched hybridizations has been explored much less. In this study, LNA- and OMeN-modified oligonucleotide probes have been prepared and their effects on the DNA duplex stability have been examined: LNA modifications can enhance the duplex stability, whereas OMeN modifications reduce the duplex stability. Next, we studied how the LNA:DNA and OMeN:DNA mismatches reduced the duplex stability. Melting temperature measurement showed that different LNA:DNA or OMeN:DNA mismatches indeed influence the duplex stability differently. LNA purines can discriminate LNA:DNA mismatches more effectively than LNA pyrimidines as well as DNA nucleotides. Furthermore, we designed five LNA- and five OMeN-modified oligonucleotide probes to simulate realistic situations where target–probe duplexes contain a complementary LNA:DNA or OMeN:DNA base pairs and a DNA:DNA mismatch simultaneously. The measured collective effect showed that the duplex stability was enhanced by the complementary LNA:DNA base pair but decreased by the DNA:DNA mismatch in a position-dependent manner regardless of the chemical identity and position of the complementary LNA:DNA base pair. On the other hand, the OMeN-modified probes also showed that the duplex stability was reduced by both the OMeN modification and the OMeN:DNA mismatch in a position-dependent manner.

  10. A modified Kelvin impact model for pounding simulation of base-isolated building with adjacent structures

    Ye Kun; Li Li; Zhu Hongping

    2009-01-01

    Base isolation can effectively reduce the seismic forces on a superstructure, particularly in low- to medium-rise buildings. However, under strong near-fault ground motions, pounding may occur at the isolation level between the base-isolated building (BIB) and its surrounding retaining walls. To effectively investigate the behavior of the BIB pounding with adjacent structures, after assessing some commonly used impact models, a modified Kelvin impact model is proposed in this paper. Relevant parameters in the modified Kelvin model are theoretically derived and numerically verified through a simple pounding case. At the same time, inelasticity of the isolated superstructure is introduced in order to accurately evaluate the potential damage to the superstructure caused by the pounding of the BIB with adjacent structures. The reliability of the modified Kelvin impact model is validated through numerical comparisons with other impact models. However, the difference between the numerical results from the various impact analytical models is not significant. Many numerical simulations of BIBs are conducted to investigate the influence of various design parameters and conditions on the peak inter-story drills and floor accelerations during pounding. It is shown that pounding can substantially increase floor accelerations, especially at the ground floor where impacts occur. Higher modes of vibration are excited during poundings, increasing the inter-story drifts instead of keeping a nearly rigid-body motion of the superstructure. Furthermore, higher ductility demands can be imposed on lower floors of the superstructure. Moreover, impact stiffness seems to play a significant role in the acceleration response at the isolation level and the inter-story drifts of lower floors of the superstructure. Finally, the numerical results show that excessive flexibility of the isolation system used to minimize the floor accelerations may cause the BIB to be more susceptible to pounding

  11. Medical image classification using spatial adjacent histogram based on adaptive local binary patterns.

    Liu, Dong; Wang, Shengsheng; Huang, Dezhi; Deng, Gang; Zeng, Fantao; Chen, Huiling

    2016-05-01

    Medical image recognition is an important task in both computer vision and computational biology. In the field of medical image classification, representing an image based on local binary patterns (LBP) descriptor has become popular. However, most existing LBP-based methods encode the binary patterns in a fixed neighborhood radius and ignore the spatial relationships among local patterns. The ignoring of the spatial relationships in the LBP will cause a poor performance in the process of capturing discriminative features for complex samples, such as medical images obtained by microscope. To address this problem, in this paper we propose a novel method to improve local binary patterns by assigning an adaptive neighborhood radius for each pixel. Based on these adaptive local binary patterns, we further propose a spatial adjacent histogram strategy to encode the micro-structures for image representation. An extensive set of evaluations are performed on four medical datasets which show that the proposed method significantly improves standard LBP and compares favorably with several other prevailing approaches. PMID:27058283

  12. The {gamma}/{gamma}{sup '} mismatch in Ni based superalloys: In situ measurements during a creep test

    Diologent, F.; Caron, P.; D' Almeida, T.; Jacques, A. E-mail: jacques@mines.u-nancy.fr; Bastie, P

    2003-01-01

    The lattice mismatch between the fcc {gamma} matrix and the ordered {gamma}{sup '} cuboiedal precipitates in superalloys induces large internal stresses within the material. These stresses have a major effect on its mechanical behaviour and on the anisotropic evolution of the microstructure (rafting...) during its lifetime. The evolution of the effective lattice mismatch of the AM1 and MCNG superalloys was measured continuously during high temperature creep tests (1100 deg. C, 150 MPa) at the ID 15 (high energy) beamline of the ESRF. The bulk profiles of the 200 reflection (parallel to the tensile axis) were recorded using the triple crystal diffractometer. Both materials have a negative mismatch, and exhibit a transition between the initial wide {gamma}{sup '} peak to a two peaks profile during stage I of the creep curve, as rafting takes place. During stage II, the 200 mismatch decreases in magnitude. During stage III, as a microstructural transition (coalescence) occurs and the strain rate increases, the mismatch changes in the same direction, but at a faster rate, while the thickness of both peaks increases. Evolution of the MCNG specimen was the same as AM1 ones, but rafting and transition to stage II take place at a different rate.

  13. Technical aspects of typing for HLA-DP alleles using allele-specific DNA in vitro amplification and sequence-specific oligonucleotide probes. Detection of single base mismatches

    Fugger, L; Morling, N; Ryder, L P;

    1990-01-01

    The polymerase chain reaction (PCR) is an effective method for in vitro DNA amplification which combined with probing with synthetic oligonucleotides can be used for, e.g., HLA-typing. We have studied the technical aspects of HLA-DP typing with the technique. DNA from mononuclear nucleated cells...... base mismatches may be detected in the PCR and typing for HLA-DP gene variants, which differ for only one base, may be performed....

  14. Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway

    Grippon, Seden; Zhao, Qiyuan; Robinson, Tom; Marshall, Jacqueline J. T.; O’Neill, Rory J.; Manning, Hugh; Kennedy, Gordon; Dunsby, Christopher; Neil, Mark; Halford, Stephen E.; French, Paul M. W.; Baldwin, Geoff S.

    2010-01-01

    Mismatch uracil DNA glycosylase (Mug) from Escherichia coli is an initiating enzyme in the base-excision repair pathway. As with other DNA glycosylases, the abasic product is potentially more harmful than the initial lesion. Since Mug is known to bind its product tightly, inhibiting enzyme turnover, understanding how Mug binds DNA is of significance when considering how Mug interacts with downstream enzymes in the base-excision repair pathway. We have demonstrated differential binding modes o...

  15. Method based on chirp decomposition for dispersion mismatch compensation in precision absolute distance measurement using swept-wavelength interferometry.

    Lu, Cheng; Liu, Guodong; Liu, Bingguo; Chen, Fengdong; Hu, Tao; Zhuang, Zhitao; Xu, Xinke; Gan, Yu

    2015-12-14

    We establish a theoretical model of dispersion mismatch in absolute distance measurements using swept-wavelength interferometry (SWI) and propose a novel dispersion mismatch compensation method called chirp decomposition. This method separates the dispersion coefficient and distance under test, which ensures dispersion mismatch compensation without introducing additional random errors. In the measurement of a target located at 3.9 m, a measurement resolution of 45.9 μm is obtained, which is close to the theoretical resolution, and a standard deviation of 0.74 μm is obtained, which is better than the traditional method. The measurement results are compared to a single-frequency laser interferometer. The target moves from 1 m to 3.7 m, and the measurement precision using the new method is less than 0.81 μm. PMID:26698959

  16. NMR study of oligonucleotides containing base pair mismatches and a human growth hormone peptide for the determination of solution structures

    Formation of unusual basepairs in DNA for random mutations in DNA was proposed in the sixties. These mismatches arise due to errors in replication, and from deamination of the 5-methylcytosine. The author's interest in studying mismatches and other oligonucleotides has been two fold. One is related to 31P chemical shifts and the backbone structure of oligonucleotides. He wanted to find out the significance of the dispersion of 31p chemical shifts in oligonucleotides. He wished to address whether this dispersion in 31P chemical shifts is related to global structural parameters of oligonucleotides like helix twist and whether he can prove the relationship between 31P chemical shifts and the backbone torsional angles epsilon and zeta. How does a mismatch affect 31P chemical shifts and the backbone torsional angle? The second interest is related to solving the three dimensional structure of these biopolymers by using NMR data (NOESY distances) and computer simulations. His major study of these mismatches has been in the assignments of the protons resonances and the phosphorus resonances by 2D NMR. He has also tried to answer the question about the relationships between 31P chemical shifts and global parameters for DNA such as the helix twist. He has made substantial progress in determination of J(H3'-P) coupling constants by 2D NMR and also in determining the relationship between the SIP chemical shifts and the backbone torsional angles by using the mismatch dodecamer sequences and the tetradecamer sequences. The 2D NMR data for the GG and GT mismatch have been used to determine three dimensional structures by using distance restrained molecular dynamics. The second project involved studying a 28 residue synthetic peptide by NMR

  17. Diagnosis of Constitutional Mismatch Repair-Deficiency Syndrome Based on Microsatellite Instability and Lymphocyte Tolerance to Methylating Agents

    Bodo, Sahra; Colas, Chrystelle; Buhard, Olivier;

    2015-01-01

    BACKGROUND & AIMS: Patients with bi-allelic germline mutations in mismatch repair (MMR) genes (MLH1, MSH2, MSH6, or PMS2) develop a rare but severe variant of Lynch syndrome called constitutional MMR deficiency (CMMRD). This syndrome is characterized by early-onset colorectal cancers, lymphomas o...

  18. Adaptive IF selection and IQ mismatch compensation in a low-IF GSM receiver

    This paper presents an algorithm that can adaptively select the intermediate frequency (IF) and compensate the IQ mismatch according to the power ratio of the adjacent channel interference to the desired signal in a low-IF GSM receiver. The IF can be adaptively selected between 100 and 130 kHz. Test result shows an improvement of phase error from 6.78° to 3.23°. Also a least mean squares (LMS) based IQ mismatch compensation algorithm is applied to improve image rejection ratio (IRR) for the desired signal along with strong adjacent channel interference. The IRR is improved from 29.1 to 44.3 dB in measurement. The design is verified in a low-IF GSM receiver fabricated in SMIC 0.13 μm RF CMOS process with a working voltage of 1.2 V. (semiconductor integrated circuits)

  19. Native and non-native speech sound processing and the neural mismatch responses: A longitudinal study on classroom-based foreign language learning.

    Jost, Lea B; Eberhard-Moscicka, Aleksandra K; Pleisch, Georgette; Heusser, Veronica; Brandeis, Daniel; Zevin, Jason D; Maurer, Urs

    2015-06-01

    Learning a foreign language in a natural immersion context with high exposure to the new language has been shown to change the way speech sounds of that language are processed at the neural level. It remains unclear, however, to what extent this is also the case for classroom-based foreign language learning, particularly in children. To this end, we presented a mismatch negativity (MMN) experiment during EEG recordings as part of a longitudinal developmental study: 38 monolingual (Swiss-) German speaking children (7.5 years) were tested shortly before they started to learn English at school and followed up one year later. Moreover, 22 (Swiss-) German adults were recorded. Instead of the originally found positive mismatch response in children, an MMN emerged when applying a high-pass filter of 3 Hz. The overlap of a slow-wave positivity with the MMN indicates that two concurrent mismatch processes were elicited in children. The children's MMN in response to the non-native speech contrast was smaller compared to the native speech contrast irrespective of foreign language learning, suggesting that no additional neural resources were committed to processing the foreign language speech sound after one year of classroom-based learning. PMID:25934634

  20. Fixed-base comb with window-non-adjacent form (NAF) method for scalar multiplication.

    Seo, Hwajeong; Kim, Hyunjin; Park, Taehwan; Lee, Yeoncheol; Liu, Zhe; Kim, Howon

    2013-01-01

    Elliptic curve cryptography (ECC) is one of the most promising public-key techniques in terms of short key size and various crypto protocols. For this reason, many studies on the implementation of ECC on resource-constrained devices within a practical execution time have been conducted. To this end, we must focus on scalar multiplication, which is the most expensive operation in ECC. A number of studies have proposed pre-computation and advanced scalar multiplication using a non-adjacent form (NAF) representation, and more sophisticated approaches have employed a width-w NAF representation and a modified pre-computation table. In this paper, we propose a new pre-computation method in which zero occurrences are much more frequent than in previous methods. This method can be applied to ordinary group scalar multiplication, but it requires large pre-computation table, so we combined the previous method with ours for practical purposes. This novel structure establishes a new feature that adjusts speed performance and table size finely, so we can customize the pre-computation table for our own purposes. Finally, we can establish a customized look-up table for embedded microprocessors. PMID:23881143

  1. Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium salts.

    Gogos, J.A.; Karayiorgou, M; Aburatani, H; Kafatos, F C

    1990-01-01

    In the presence of tetramethylammonium chloride, potassium permanganate specifically modifies mismatched thymines. Similarly, the modification of mismatched cytosines by hydroxylamine was enhanced by tetraethylammonium chloride. Modification followed by piperidine cleavage permits specific identification of the T and C mismatches and by extension, when the opposite DNA strand is analyzed, of A and G mismatches as well. These reactions can be performed conveniently with DNA immobilized on Hybo...

  2. Zero energy buildings and mismatch compensation factors

    Lund, Henrik; Marszal, Anna Joanna; Heiselberg, Per

    2011-01-01

    individual buildings and an element of economy of scale. For these three reasons mismatches should be dealt with at the aggregated level and not at the individual level of each building. Instead, this paper suggests to compensate the mismatch of a building by increasing (or decreasing) the capacity of the...... energy production unit. Based on historical data for the electricity supply area in western Denmark, this paper makes a first attempt to quantify mismatch compensation factors. The results indicate that such compensation factors are a little below one for buildings with photovoltaics (PV) and a little...

  3. A Mismatch EndoNuclease Array-Based Methodology (MENA for Identifying Known SNPs or Novel Point Mutations

    Josep M. Comeron

    2016-04-01

    Full Text Available Accurate and rapid identification or confirmation of single nucleotide polymorphisms (SNPs, point mutations and other human genomic variation facilitates understanding the genetic basis of disease. We have developed a new methodology (called MENA (Mismatch EndoNuclease Array pairing DNA mismatch endonuclease enzymology with tiling microarray hybridization in order to genotype both known point mutations (such as SNPs as well as identify previously undiscovered point mutations and small indels. We show that our assay can rapidly genotype known SNPs in a human genomic DNA sample with 99% accuracy, in addition to identifying novel point mutations and small indels with a false discovery rate as low as 10%. Our technology provides a platform for a variety of applications, including: (1 genotyping known SNPs as well as confirming newly discovered SNPs from whole genome sequencing analyses; (2 identifying novel point mutations and indels in any genomic region from any organism for which genome sequence information is available; and (3 screening panels of genes associated with particular diseases and disorders in patient samples to identify causative mutations. As a proof of principle for using MENA to discover novel mutations, we report identification of a novel allele of the beethoven (btv gene in Drosophila, which encodes a ciliary cytoplasmic dynein motor protein important for auditory mechanosensation.

  4. Research of China’s Credit Currency Mismatch

    Rui Cui; Weiyi Wang

    2010-01-01

    Large positive current mismatch exists in China at present, and with the continual development of financial reform, the risk of the current mismatch will gradually diffuse from the micro layer to the macro layer, so it is very important to study China’s credit currency mismatch. Based on the theoretical research of the credit currency mismatch, the formula of AECM is used to measure the level of China’s credit currency mismatch, and the causes are analyzed, and corresponding policy advices ar...

  5. Influence of mis-match of weld and base material creep properties on elevated temperature design of pressure vessels and piping

    The stress and strain concentrations developed at the weldments during the long time operation of pressure vessels and piping at high temperature due to the mis-match in the creep properties of weldment constituents (weld, heat affected zone and base metal) are estimated using detailed finite element analysis. Three materials, viz. 2.25Cr 1Mo, SS 316 LN and modified 9Cr 1 Mo which are the most commonly used materials in the nuclear and thermal power plants are considered. A longitudinal seam weld with single and double V (X) configurations are analysed. Parametric studies have been done on weld angle and stresses. Based on the analysis, critical locations and the maximum stress concentration factors in the weldments for the above materials are identified. The weld design procedures of the currently used pressure vessel and piping codes are commented. The importance of ductility based failure criteria is emphasised. (orig.)

  6. $p$-th Clustering coefficients $C_{p}$ and Adjacent Matrix for Networks: Formulation based on String

    Toyota, Norihito

    2009-01-01

    The phenomenon of six degrees of separation is an old but interesting problem. The considerations of the clustering coefficient reflecting triangular structures and its extension to square one to six degrees of separation have been made\\cite{Newm21}. Recently, Aoyama\\cite{Aoyama} has given some considerations to this problem in networks without loops, using a sort of general formalism, "string formalism". In this article, we describe relations between the string formulation proposed by Aoyama and an adjacent matrix. Thus we provided a reformulation of the string formulation proposed by \\cite{Aoyama} to analyze networks. According to it, we introduced a series of generalized $q$-$th$ clustering coefficients. The available rules between diagrams of graphs and formulae are also given based on the formulation. Next we apply the formulation to some subjects in order to mainly check consistency with former studies. By evaluating the clustering coefficient for typical networks studied well earlier, we confirm a vali...

  7. MutS recognition: Multiple mismatches and sequence context effects

    Amita Joshi; Basuthkar J Rao

    2001-12-01

    Escherichia coli MutS is a versatile repair protein that specifically recognizes not only various types of mismatches but also single stranded loops of up to 4 nucleotides in length. Specific binding, followed by the next step of tracking the DNA helix that locates hemi-methylated sites, is regulated by the conformational state of the protein as a function of ATP binding/hydrolysis. Here, we study how various molecular determinants of a heteroduplex regulate mismatch recognition by MutS, the critical first step of mismatch repair. Using classical DNase I footprinting assays, we demonstrate that the hierarchy of MutS binding to various types of mismatches is identical whether the mismatches are present singly or in multiples. Moreover, this unique hierarchy is indifferent both to the differential level of DNA helical flexibility and to the unpaired status of the mismatched bases in a heteroduplex. Surprisingly, multiple mismatches exhibit reduced affinity of binding to MutS, compared to that of a similar single mismatch. Such a reduction in the affinity might be due to sequence context effects, which we established more directly by studying two identical single mismatches in an altered sequence background. A mismatch, upon simply being flipped at the same location, elicits changes in MutS specific contacts, thereby underscoring the importance of sequence context in modulating MutS binding to mismatches.

  8. Strategic Mismatches in Competing Teams

    Kräkel, Matthias

    1999-01-01

    This paper discusses the strategic role of mismatching, where players voluntarily form inefficient teams or forego the formation of efficient teams, respectively. Strategic mismatching can be rational when players realize a competitive advantage (e.g. harming other competitors). In addition, the results show that free riding can be beneficial for a team in combination with strategic mismatching and that the loser?s curse may be welfare improving by mitigating the problem of strategic mismatch...

  9. Human DNA mismatch repair: coupling of mismatch recognition to strand-specific excision

    WANG Huixian; Hays, John B.

    2007-01-01

    Eukaryotic mismatch-repair (MMR) proteins MutSα and MutLα couple recognition of base mismatches to strand-specific excision, initiated in vivo at growing 3′ ends and 5′ Okazaki-fragment ends or, in human nuclear extracts, at nicks in exogenous circular substrates. We addressed five biochemical questions relevant to coupling models. Excision remained fully efficient at DNA:MutSα ratios of nearly 1 to 1 at various mismatch-nick distances, suggesting a requirement for only one MutSα molecule per...

  10. Anomalous cross-linking by mechlorethamine of DNA duplexes containing C-C mismatch pairs.

    Romero, R M; Mitas, M; Haworth, I S

    1999-03-23

    Nitrogen mustards such as mechlorethamine have previously been shown to covalently cross-link DNA through the N7 position of the two guanine bases of a d[GXC].d[GYC] duplex sequence, a so-called 1,3 G-G-cross-link, when X-Y = C-G or T-A. Here, we report the formation of a new mechlorethamine cross-link with the d[GXC].d[GYC] fragment when X-Y is a C-C mismatch pair. Mechlorethamine cross-links this fragment preferentially between the two mismatched cytosine bases, rather than between the guanine bases. The cross-link also forms when one or both of the guanine bases of the d[GCC].d[GCC] fragment are replaced by N7-deazaguanine, and, more generally, forms with any C-C mismatch, regardless of the flanking base pairs. Piperidine cleavage of the cross-link species containing the d[GCC].d[GCC] sequence gives DNA fragments consistent with alkylation at the mismatched cytosine bases. We also provide evidence that the cross-link reaction occurs between the N3 atoms of the two cytosine bases by showing that the formation of the C-C cross-link is pH dependent for both mechlorethamine and chlorambucil. Dimethyl sulfate (DMS) probing of the cross-linked d[GCC].d[GCC] fragment showed that the major groove of the guanine adjacent to the C-C mismatch is still accessible to DMS. In contrast, the known minor groove binder Hoechst 33258 inhibits the cross-link formation with a C-C mismatch pair flanked by A-T base pairs. These results suggest that the C-C mismatch is cross-linked by mechlorethamine in the minor groove. Since C-C pairs may be involved in unusual secondary structures formed by the trinucleotide repeat sequence d[CCG]n, and associated with triplet repeat expansion diseases, mechlorethamine may serve as a useful probe for these structures. PMID:10090751

  11. An analytical study of mismatched complementary media

    Zhu, Lin; Ma, Hongru

    2016-01-01

    Complementary media (CM) interacting with arbitrarily situated obstacles are usually less discussed. In this paper, an analytical framework based on multiple scattering theory is established for analyzing such a mismatched case. As examples, CM-based devices, i.e., a superlens and superscatterer, are discussed. From an analysis, the cancellation mechanism of the mismatched CM is studied. In addition, numerical results are provided for illustration. Moreover, further study shows that such cancellation effects might rely on specific conditions. Actually, the conclusions are not restricted to any specific frequencies; they could be extended to many other areas including applications to active cloaking, antennas, and wireless power transfer.

  12. Mammalian mismatch repair

    Pena Diaz, Javier; Jiricny, Josef

    2012-01-01

    A considerable surge of interest in the mismatch repair (MMR) system has been brought about by the discovery of a link between Lynch syndrome, an inherited predisposition to cancer of the colon and other organs, and malfunction of this key DNA metabolic pathway. This review focuses on recent...... advances in our understanding of the molecular mechanisms of canonical MMR, which improves replication fidelity by removing misincorporated nucleotides from the nascent DNA strand. We also discuss the involvement of MMR proteins in two other processes: trinucleotide repeat expansion and antibody maturation......, in which MMR proteins are required for mutagenesis rather than for its prevention....

  13. PARP-1 enhances the mismatch-dependence of 5′-directed excision in human mismatch repair in vitro

    Liu, Yiyong; Kadyrov, Farid A; Modrich, Paul

    2011-01-01

    End-directed mismatch-provoked excision has been reconstituted in several purified systems. While 3′-directed excision displays a mismatch dependence similar to that observed in nuclear extracts (≈ 20-fold), the mismatch dependence of 5′-directed excision is only 3 to 4-fold, significantly less than that in extracts (8 to 10-fold). Utilizing a fractionation-based approach, we have isolated a single polypeptide that enhances mismatch dependence of reconstituted 5′-directed excision and have sh...

  14. Mechanisms in E. coli and Human Mismatch Repair (Nobel Lecture).

    Modrich, Paul

    2016-07-18

    DNA molecules are not completely stable, they are subject to chemical or photochemical damage and errors that occur during DNA replication resulting in mismatched base pairs. Through mechanistic studies Paul Modrich showed how replication errors are corrected by strand-directed mismatch repair in Escherichia coli and human cells. PMID:27198632

  15. Potential for DNA-based identification of Great Lakes fauna: Match and mismatch between taxa inventories and DNA barcode libraries

    DNA-based identification of mixed-organism samples offers the potential to greatly reduce the need for resource-intensive morphological identification, which would be of value both to biotic condition assessment and non-native species early-detection monitoring. However, the abi...

  16. GPS-corrected and GIS-based remapping of the Kalahari Gemsbok National Park and the adjacent area in Botswana

    J. du P. Bothma

    1993-09-01

    Full Text Available GPS-equipment was used to map the interior roads, major pans and the location of all windmills and solar-equipped boreholes in the Kalahari Gemsbok National Park and the adjacent areas of Botswana. The final map was generated with GIS-equipment, and supplies managers and planners with the first error-free map of the area. The major errors of previous maps are indicated.

  17. Metrology of ground-based satellite validation: co-location mismatch and smoothing issues of total ozone comparisons

    Verhoelst, T.; Granville, J.; Hendrick, F.; Köhler, U.; Lerot, C.; Pommereau, J.-P.; Redondas, A.; Van Roozendael, M.; Lambert, J.-C.

    2015-12-01

    Comparisons with ground-based correlative measurements constitute a key component in the validation of satellite data on atmospheric composition. The error budget of these comparisons contains not only the measurement errors but also several terms related to differences in sampling and smoothing of the inhomogeneous and variable atmospheric field. A versatile system for Observing System Simulation Experiments (OSSEs), named OSSSMOSE, is used here to quantify these terms. Based on the application of pragmatic observation operators onto high-resolution atmospheric fields, it allows a simulation of each individual measurement, and consequently, also of the differences to be expected from spatial and temporal field variations between both measurements making up a comparison pair. As a topical case study, the system is used to evaluate the error budget of total ozone column (TOC) comparisons between GOME-type direct fitting (GODFITv3) satellite retrievals from GOME/ERS2, SCIAMACHY/Envisat, and GOME-2/MetOp-A, and ground-based direct-sun and zenith-sky reference measurements such as those from Dobsons, Brewers, and zenith-scattered light (ZSL-)DOAS instruments, respectively. In particular, the focus is placed on the GODFITv3 reprocessed GOME-2A data record vs. the ground-based instruments contributing to the Network for the Detection of Atmospheric Composition Change (NDACC). The simulations are found to reproduce the actual measurements almost to within the measurement uncertainties, confirming that the OSSE approach and its technical implementation are appropriate. This work reveals that many features of the comparison spread and median difference can be understood as due to metrological differences, even when using strict co-location criteria. In particular, sampling difference errors exceed measurement uncertainties regularly at most mid- and high-latitude stations, with values up to 10 % and more in extreme cases. Smoothing difference errors only play a role in the

  18. Metrology of ground-based satellite validation: co-location mismatch and smoothing issues of total ozone comparisons

    T. Verhoelst

    2015-12-01

    Full Text Available Comparisons with ground-based correlative measurements constitute a key component in the validation of satellite data on atmospheric composition. The error budget of these comparisons contains not only the measurement errors but also several terms related to differences in sampling and smoothing of the inhomogeneous and variable atmospheric field. A versatile system for Observing System Simulation Experiments (OSSEs, named OSSSMOSE, is used here to quantify these terms. Based on the application of pragmatic observation operators onto high-resolution atmospheric fields, it allows a simulation of each individual measurement, and consequently, also of the differences to be expected from spatial and temporal field variations between both measurements making up a comparison pair. As a topical case study, the system is used to evaluate the error budget of total ozone column (TOC comparisons between GOME-type direct fitting (GODFITv3 satellite retrievals from GOME/ERS2, SCIAMACHY/Envisat, and GOME-2/MetOp-A, and ground-based direct-sun and zenith–sky reference measurements such as those from Dobsons, Brewers, and zenith-scattered light (ZSL-DOAS instruments, respectively. In particular, the focus is placed on the GODFITv3 reprocessed GOME-2A data record vs. the ground-based instruments contributing to the Network for the Detection of Atmospheric Composition Change (NDACC. The simulations are found to reproduce the actual measurements almost to within the measurement uncertainties, confirming that the OSSE approach and its technical implementation are appropriate. This work reveals that many features of the comparison spread and median difference can be understood as due to metrological differences, even when using strict co-location criteria. In particular, sampling difference errors exceed measurement uncertainties regularly at most mid- and high-latitude stations, with values up to 10 % and more in extreme cases. Smoothing difference errors only

  19. Potential for DNA-based identification of Great Lakes fauna: match and mismatch between taxa inventories and DNA barcode libraries

    Trebitz, Anett S.; Hoffman, Joel C.; Grant, George W.; Billehus, Tyler M.; Pilgrim, Erik M.

    2015-01-01

    DNA-based identification of mixed-organism samples offers the potential to greatly reduce the need for resource-intensive morphological identification, which would be of value both to bioassessment and non-native species monitoring. The ability to assign species identities to DNA sequences found depends on the availability of comprehensive DNA reference libraries. Here, we compile inventories for aquatic metazoans extant in or threatening to invade the Laurentian Great Lakes and examine the availability of reference mitochondrial COI DNA sequences (barcodes) in the Barcode of Life Data System for them. We found barcode libraries largely complete for extant and threatening-to-invade vertebrates (100% of reptile, 99% of fish, and 92% of amphibian species had barcodes). In contrast, barcode libraries remain poorly developed for precisely those organisms where morphological identification is most challenging; 46% of extant invertebrates lacked reference barcodes with rates especially high among rotifers, oligochaetes, and mites. Lack of species-level identification for many aquatic invertebrates also is a barrier to matching DNA sequences with physical specimens. Attaining the potential for DNA-based identification of mixed-organism samples covering the breadth of aquatic fauna requires a concerted effort to build supporting barcode libraries and voucher collections. PMID:26199185

  20. Nonlinear disturbance observer-based control for multi-input multi-output nonlinear systems subject to mismatching condition

    Yang, Jun; Li, Shihua; Chen, Wen-Hua

    2012-08-01

    For a multi-input multi-output (MIMO) nonlinear system, the existing disturbance observer-based control (DOBC) only provides solutions to those whose disturbance relative degree (DRD) is higher than or equal to its input relative degree. By designing a novel disturbance compensation gain matrix, a generalised nonlinear DOBC method is proposed in this article to solve the disturbance attenuation problem of the MIMO nonlinear system with arbitrary DRD. It is shown that the disturbances are able to be removed from the output channels by the proposed method with appropriately chosen control parameters. The property of nominal performance recovery, which is the major merit of the DOBCs, is retained with the proposed method. The feasibility and effectiveness of the proposed method are demonstrated by simulation studies of both the numerical and application examples.

  1. Streptococcus mutans counts in plaque adjacent to orthodontic brackets bonded with resin-modified glass ionomer cement or resin-based composite

    Solange Machado Mota

    2008-03-01

    Full Text Available This study investigated the number of Streptococcus mutans CFU (colony forming units in the saliva and plaque adjacent to orthodontic brackets bonded with a glass ionomer cement - GIC (Fuji Ortho or a resin-based composite - RC (Concise. Twenty male and female patients, aged 12 to 20 years, participated in the study. Saliva was collected before and after placement of appliances. Plaque was collected from areas adjacent to brackets and saliva was again collected on the 15th, 30th, and 45th day after placement. On the 30th day, 0.4% stannous fluoride gel was applied for 4 minutes. No significant modification in the number of Streptococcus mutans CFU in saliva was observed after placement of the fixed orthodontic appliances. On the 15th day, the percentage of Streptococcus mutans CFU in plaque was statistically lower in sites adjacent to GIC-bonded brackets (mean = 0.365 than in those adjacent to RC-bonded brackets (mean = 0.935. No evidence was found of a contribution of GIC to the reduction of CFU in plaque after the 15th day. Topical application of stannous fluoride gel on the 30th day reduced the number of CFU in saliva, but not in plaque. This study suggests that the antimicrobial activity of GIC occurs only in the initial phase and is not responsible for a long-term anticariogenic property.

  2. Heterogenous mismatch-repair status in colorectal cancer

    Joost, Patrick; Veurink, Nynke; Holck, Susanne;

    2014-01-01

    BACKGROUND: Immunohistochemical staining for mismatch repair proteins is efficient and widely used to identify mismatch repair defective tumors. The tumors typically show uniform and widespread loss of MMR protein staining. We identified and characterized colorectal cancers with alternative......, heterogenous mismatch repair protein staining in order to delineate expression patterns and underlying mechanisms. METHODS: Heterogenous staining patterns that affected at least one of the mismatch repair proteins MLH1, PMS2, MSH2 and MSH6 were identified in 14 colorectal cancers. Based on alternative...... expression patterns macro-dissected and micro-dissected tumor areas were separately analyzed for microsatellite instability and MLH1 promoter methylation. RESULTS: Heterogenous retained/lost mismatch repair protein expression could be classified as intraglandular (within or in-between glandular formations...

  3. Design and analysis of mismatch probes for long oligonucleotide microarrays

    Deng, Ye; He, Zhili; Van Nostrand, Joy D.; Zhou, Jizhong

    2008-08-15

    Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this approach has not been used for longer oligonucleotide probes. Here, an oligonucleotide microarray was constructed to evaluate and optimize parameters for 50-mer mismatch probe design. A perfect match (PM) and 28 mismatch (MM) probes were designed for each of ten target genes selected from three microorganisms. The microarrays were hybridized with synthesized complementary oligonucleotide targets at different temperatures (e.g., 42, 45 and 50 C). In general, the probes with evenly distributed mismatches were more distinguishable than those with randomly distributed mismatches. MM probes with 3, 4 and 5 mismatched nucleotides were differentiated for 50-mer oligonucleotide probes hybridized at 50, 45 and 42 C, respectively. Based on the experimental data generated from this study, a modified positional dependent nearest neighbor (MPDNN) model was constructed to adjust the thermodynamic parameters of matched and mismatched dimer nucleotides in the microarray environment. The MM probes with four flexible positional mismatches were designed using the newly established MPDNN model and the experimental results demonstrated that the redesigned MM probes could yield more consistent hybridizations. Conclusions: This study provides guidance on the design of MM probes for long oligonucleotides (e.g., 50 mers). The novel MPDNN model has improved the consistency for long MM probes, and this modeling method can potentially be used for the prediction of oligonucleotide microarray hybridizations.

  4. Adjacent segment disease.

    Virk, Sohrab S; Niedermeier, Steven; Yu, Elizabeth; Khan, Safdar N

    2014-08-01

    EDUCATIONAL OBJECTIVES As a result of reading this article, physicians should be able to: 1. Understand the forces that predispose adjacent cervical segments to degeneration. 2. Understand the challenges of radiographic evaluation in the diagnosis of cervical and lumbar adjacent segment disease. 3. Describe the changes in biomechanical forces applied to adjacent segments of lumbar vertebrae with fusion. 4. Know the risk factors for adjacent segment disease in spinal fusion. Adjacent segment disease (ASD) is a broad term encompassing many complications of spinal fusion, including listhesis, instability, herniated nucleus pulposus, stenosis, hypertrophic facet arthritis, scoliosis, and vertebral compression fracture. The area of the cervical spine where most fusions occur (C3-C7) is adjacent to a highly mobile upper cervical region, and this contributes to the biomechanical stress put on the adjacent cervical segments postfusion. Studies have shown that after fusion surgery, there is increased load on adjacent segments. Definitive treatment of ASD is a topic of continuing research, but in general, treatment choices are dictated by patient age and degree of debilitation. Investigators have also studied the risk factors associated with spinal fusion that may predispose certain patients to ASD postfusion, and these data are invaluable for properly counseling patients considering spinal fusion surgery. Biomechanical studies have confirmed the added stress on adjacent segments in the cervical and lumbar spine. The diagnosis of cervical ASD is complicated given the imprecise correlation of radiographic and clinical findings. Although radiological and clinical diagnoses do not always correlate, radiographs and clinical examination dictate how a patient with prolonged pain is treated. Options for both cervical and lumbar spine ASD include fusion and/or decompression. Current studies are encouraging regarding the adoption of arthroplasty in spinal surgery, but more long

  5. Effects of Mismatched Parameter on Chaotic Synchronization

    PENGJiang-hua; FANGJin-qing

    2003-01-01

    Chaos-based security communication has become one of the most interesting hot subjects for research of chaotic theory in real world since. In recent years, secure communication via synchronized chaos has been intensely studied. However, in practical application it is difficult to construct two complete identical chaotic systems since there are many reasons to induce parameter mismatch between two systems (response system and drive system).

  6. Robust Control of Urban Industrial Water Mismatching Uncertain System

    LI Kebai

    2013-02-01

    Full Text Available Urban industrial water system parameter fluctuation producing uncertainty may not occur in a control input channel, can be applied mismatching uncertain system to describe. Based on Lyapunov direct method and linear matrix inequality, design the urban industrial water mismatching uncertain system feedback stabilization robust control scheme. Avoid the defects that the feedback stabilization control method based on the matrix Riccati equation need to preset equation parameters, easier to solve and can reduce the conservative.

  7. The Effect of Basepair Mismatch on DNA Strand Displacement.

    Broadwater, D W Bo; Kim, Harold D

    2016-04-12

    DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized. Here, we measured toehold-mediated strand displacement kinetics using single-molecule fluorescence in the presence of a single basepair mismatch. The apparent displacement rate varied significantly when the mismatch was introduced in the invading DNA strand. The rate generally decreased as the mismatch in the invader was encountered earlier in displacement. Our data indicate that a single base pair mismatch in the invader stalls branch migration and displacement occurs via direct dissociation of the destabilized incumbent strand from the substrate strand. We combined both branch migration and direct dissociation into a model, which we term the concurrent displacement model, and used the first passage time approach to quantitatively explain the salient features of the observed relationship. We also introduce the concept of splitting probabilities to justify that the concurrent model can be simplified into a three-step sequential model in the presence of an invader mismatch. We expect our model to become a powerful tool to design DNA-based reaction schemes with broad functionality. PMID:27074674

  8. Fast inter-mode decision algorithm for high-efficiency video coding based on similarity of coding unit segmentation and partition mode between two temporally adjacent frames

    Zhong, Guo-Yun; He, Xiao-Hai; Qing, Lin-Bo; Li, Yuan

    2013-04-01

    High-efficiency video coding (HEVC) introduces a flexible hierarchy of three block structures: coding unit (CU), prediction unit (PU), and transform unit (TU), which have brought about higher coding efficiency than the current national video coding standard H.264/advanced video coding (AVC). HEVC, however, simultaneously requires higher computational complexity than H.264/AVC, although several fast inter-mode decisions were proposed in its development. To further reduce this complexity, a fast inter-mode decision algorithm is proposed based on temporal correlation. Because of the distinct difference of inter-prediction block between HEVC and H.264/AVC, in order to use the temporal correlation to speed up the inter prediction, the correlation of inter-prediction between two adjacent frames needs to be analyzed according to the structure of CU and PU in HEVC. The probabilities of all the partition modes in all sizes of CU and the similarity of CU segmentation and partition modes between two adjacent frames are tested. The correlation of partition modes between two CUs with different sizes in two adjacent frames is tested and analyzed. Based on the characteristics tested and analyzed, at most, two prior partition modes are evaluated for each level of CU, which reduces the number of rate distortion cost calculations. The simulation results show that the proposed algorithm further reduces coding time by 33.0% to 43.3%, with negligible loss in bitrate and peak signal-to-noise ratio, on the basis of the fast inter-mode decision algorithms in current HEVC reference software HM7.0.

  9. Elastic-plastic fracture mechanics of strength-mismatching

    Parks, D.M.; Ganti, S.; McClintock, F.A. [Massachusetts Institute of Technology, Cambridge, MA (United States)

    1996-12-31

    Approximate solutions to stress-fields are provided for a strength-mismatched interface crack in small-scale yielding (SSY) for non-hardening and low hardening materials. Variations of local deformation intensities, characterized by a J-type contour integral, are proposed. The softer material experiences a higher deformation intensity level, J{sub S}, while the harder material sees a much lower deformation intensity level, J{sub H}, compared to that obtained from the applied J near the respective homogeneous crack-tips. For a low hardening material, the stress fields are obtained by scaling from an elastic/perfectly-plastic problem, based on an effective mismatch, M{sub eff}, which is a function of mismatch, M, and the hardening exponent, n. Triaxial stress build-up is discussed quantitatively in terms of M. The influence of strength-mismatch on cleavage fracture is discussed using Weibull statistics.

  10. Reverse ventilation--perfusion mismatch

    Patients having lobar airway obstruction or consolidation usually have decreases of both ventilation and perfusion on lung scans. We report three patients in whom hypoxic vasoconstriction was apparently incomplete, resulting in a ''reversed'' ventilation-perfusion mismatch. Perfusion of the hypoxic lobe on the radionuclide scan was associated with metabolic alkalosis, pulmonary venous and pulmonary arterial hypertension in these patients

  11. Gordian adjacency for torus knots

    Feller, Peter

    2013-01-01

    A knot K is called Gordian adjacent to a knot L if there exists an unknotting sequence for L containing K. We provide a sufficient condition for Gordian adjacency of torus knots via the study of knots in the thickened torus. We also completely describe Gordian adjacency for torus knots of index 2 and 3 using Levine-Tristram signatures as obstructions to Gordian adjacency. Finally, Gordian adjacency for torus knots is compared to the notion of adjacency for plane curve singularities.

  12. Seismic structure beneath the Gulf of Aqaba and adjacent areas based on the tomographic inversion of regional earthquake data

    El Khrepy, Sami; Koulakov, Ivan; Al-Arifi, Nassir; Petrunin, Alexey G.

    2016-06-01

    We present the first 3-D model of seismic P and S velocities in the crust and uppermost mantle beneath the Gulf of Aqaba and surrounding areas based on the results of passive travel time tomography. The tomographic inversion was performed based on travel time data from ˜ 9000 regional earthquakes provided by the Egyptian National Seismological Network (ENSN), and this was complemented with data from the International Seismological Centre (ISC). The resulting P and S velocity patterns were generally consistent with each other at all depths. Beneath the northern part of the Red Sea, we observed a strong high-velocity anomaly with abrupt limits that coincide with the coastal lines. This finding may indicate the oceanic nature of the crust in the Red Sea, and it does not support the concept of gradual stretching of the continental crust. According to our results, in the middle and lower crust, the seismic anomalies beneath the Gulf of Aqaba seem to delineate a sinistral shift (˜ 100 km) in the opposite flanks of the fault zone, which is consistent with other estimates of the left-lateral displacement in the southern part of the Dead Sea Transform fault. However, no displacement structures were visible in the uppermost lithospheric mantle.

  13. Mismatch Negativity: Translating the Potential

    JuanitaTodd

    2013-01-01

    The mismatch negativity (MMN) component of the auditory event-related potential has become a valuable tool in cognitive neuroscience. Its reduced size in persons with schizophrenia is of unknown origin but theories proposed include links to problems in experience-dependent plasticity reliant on N-methyl-d-aspartate glutamate receptors. In this review we address the utility of this tool in revealing the nature and time course of problems in perceptual inference in this illness together with it...

  14. Educational Mismatch and Wait Unemployment

    Patrizia Ordine; Giuseppe Rose

    2011-01-01

    This work investigates educational mismatch and its interrelationships with individual unemployment duration. By studying unemployment histories of Italian workers we show that overeducated have longer unemployment spells than well matched workers. Using duration models we show that hazard rates of graduates are higher than those of undergraduates only for transitions toward occupations that require the competencies provided by the universities. This process is strictly related to innate abil...

  15. Even aberration measurement of lithographic projection optics based on intensity difference of adjacent peaks in alternating phase-shifting mask image

    We propose an in situ technique for measuring an even aberration of lithographic projection optics. By using the Hopkins theory of partially coherent imaging and the thick-mask model, the linear relationship between the intensity difference of adjacent peaks in an alternating phase-shifting mask image and an even aberration is established by equations and verified by numerical results. The sensitivity of measuring the even aberration of lithographic projection optics based on this linear relationship is analyzed, and the measurement mark is designed accordingly. Measurement performance of the present technique is evaluated using the lithographic simulator PROLITH, which shows that the present technique is capable of measuring the even aberration of lithographic projection optics with ultrahigh measurement accuracy.

  16. $p$-th Clustering coefficients and $q$-$th$ degrees of separation based on String-Adjacent Formulation

    Toyota, Norihito

    2010-01-01

    The phenomenon of six degrees of separation is an old but attractive subject. The deep understanding has been uncovered yet, especially how closed paths included in a network affect six degrees of separation are an important subject left yet. For it, some researches have been made\\cite{Newm21}, \\cite{Aoyama}. Recently we have develop a formalism \\cite{Toyota3},\\cite{Toyota4} to explore the subject based on the string formalism developed by Aoyama\\cite{Aoyama}. The formalism can systematically investigate the effect of closed paths, especially generalized clustering coefficient $C_{(p)}$ introduced in \\cite{Toyota4}, on six degrees of separation. In this article, we analyze general $q$-th degrees of separation by using the formalism developed by us. So we find that the scale free network with exponent $\\gamma=3$ just display six degrees of separation. Furthermore we drive a phenomenological relation between the separation number $q$ and $C_{(p)}$ that has crucial information on circle structures in networks.

  17. Chimeric Proteins to Detect DNA Damage and Mismatches

    McCutchen-Maloney, S; Malfatti, M; Robbins, K M

    2002-01-14

    The goal of this project was to develop chimeric proteins composed of a DNA mismatch or damage binding protein and a nuclease, as well as methods to detect DNA mismatches and damage. We accomplished this through protein engineering based on using polymerase chain reactions (PCRs) to create chimeras with novel functions for damage and mismatch detection. This project addressed fundamental questions relating to disease susceptibility and radiation-induced damage in cells. It also supported and enhanced LLNL's competency in the emerging field of proteomics. In nature, DNA is constantly being subjected to damaging agents such as exposure to ultraviolet (UV) radiation and various environmental and dietary carcinogens. If DNA damage is not repaired however, mutations in DNA result that can eventually manifest in cancer and other diseases. In addition to damage-induced DNA mutations, single nucleotide polymorphisms (SNPs), which are variations in the genetic sequence between individuals, may predispose some to disease. As a result of the Human Genome Project, the integrity of a person's DNA can now be monitored. Therefore, methods to detect DNA damage, mutations, and SNPs are useful not only in basic research but also in the health and biotechnology industries. Current methods of detection often use radioactive labeling and rely on expensive instrumentation that is not readily available in many research settings. Our methods to detect DNA damage and mismatches employ simple gel electrophoresis and flow cytometry, thereby alleviating the need for radioactive labeling and expensive equipment. In FY2001, we explored SNP detection by developing methods based on the ability of the chimeric proteins to detect mismatches. Using multiplex assays with flow cytometry and fluorescent beads to which the DNA substrates where attached, we showed that several of the chimeras possess greater affinity for damaged and mismatched DNA than for native DNA. This affinity was

  18. A Compression Method for ECG Based on DCT of Difference between Two Adjacent Frames%帧间差值的DCT压缩ECG数据的方法

    王培康; 费小英

    2000-01-01

    心电图(ECG)信号具有准周期性,各个心动周期(称其为帧)的ECG波形极为相似,将相邻两帧的ECG波形数据相减,得到差值数据的波形比原始ECG波形平坦、数据值小,差值数据的DCT有效分量更为集中在低频段,且这些分量的幅度较低,由此,可以得到更高的数据压缩比。因此本文提出了一种利用ECG帧间差值代替原始ECG信号进行DCT变换,并用双码长编码方法对DCT分量进行编码的数据压缩方法。用该方法得到的重构ECG波形具有良好的保真度。%Since most of the DCT coefficients of an initial ECG signal are spread in low frequency,so is the energy of the signal, we can get a higher compression ratio (CR) by distributing DCT coefficients in a smaller region of low frequency and by degrading the amplitude of main DCT coefficients. ECG is a quasi-periodic signal with strong correlation between adjacent frames, so the difference sequence between them is more smooth and has lower amplitudes. Based on this property, a method of ECG data compression via DCT is presented in this paper, and it replaces the DCT coefficients of the original signal with the DCT coefficients of difference between two adjacent frames of the original signal and the DCT coefficients are encoded into two kinds of codes with different lengths. The quality of the reconstructed signal got by this way is high.

  19. Seasonal changes in phytoplankton biomass and dominant species in the Changjiang River Estuary and adjacent seas: General trends based on field survey data 1959-2009

    Yang, Shu; Han, Xiurong; Zhang, Chuansong; Sun, Baiye; Wang, Xiulin; Shi, Xiaoyong

    2014-12-01

    The characteristics of seasonal variation in phytoplankton biomass and dominant species in the Changjiang River Estuary and adjacent seas were discussed based on field investigation data from 1959 to 2009. The field data from 1981 to 2004 showed that the Chlorophyll- a concentration in surface seawater was between 0.4 and 8.5 μg dm-3. The seasonal changes generally presented a bimodal trend, with the biomass peaks occurring in May and August, and Chlorophyll-a concentration was the lowest in winter. Seasonal biomass changes were mainly controlled by temperature and nutrient levels. From the end of autumn to the next early spring, phytoplankton biomass was mainly influenced by temperature, and in other seasons, nutrient level (including the nutrient supply from the terrestrial runoffs) was the major influence factor. Field investigation data from 1959 to 2009 demonstrated that diatoms were the main phytoplankton in this area, and Skeletonema costatum, Pseudo-nitzschia pungens, Coscinodiscus oculus-iridis, Thalassinoema nitzschioides, Paralia sulcata, Chaetoceros lorenzianus, Chaetoceros curvisetus, and Prorocentrum donghaiense Lu were common dominant species. The seasonal variations in major dominant phytoplankton species presented the following trends: 1) Skeletonema (mainly S. costatum) was dominant throughout the year; and 2) seasonal succession trends were Coscinodiscus (spring) → Chaetoceros (summer and autumn) → Coscinodiscus (winter). The annual dominance of S. costatum was attributed to its environmental eurytopicity and long standing time in surface waters. The seasonal succession of Coscinodiscus and Chaetoceros was associated with the seasonal variation in water stability and nutrient level in this area. On the other hand, long-term field data also indicated obvious interannual variation of phytoplankton biomass and community structure in the Changjiang River Estuary and adjacent seas: average annual phytoplankton biomass and dinoflagellate

  20. Ecological mapping and data quality assessment for the needs of ecosystem-based marine spatial management: case study Greek Ionian Sea and the adjacent gulfs

    Y. ISSARIS

    2013-01-01

    Full Text Available Mapping of ecosystem components (natural and socioeconomic is a prerequisite for ecosystem-based marine spatial management (EB-MSM. To initiate the process of EB-MSM in the Greek Ionian Sea and the adjacent gulfs, the main relevant ecosystem components were mapped based on existing spatial information and expert judgment. The natural components mapped included habitat types and species targeted for conservation, according to national and European legislation and international agreements. Main human activities/pressures related to fisheries, aquaculture, tourism, and industry were also mapped. In order to assess the quality of data used to map ecosystem components and therefore take into consideration the inherent uncertainty, an assessment of 5 semi-quantitative data indicators based on a pedigree matrix was conducted. Through this qualitative approach we gained information related to the sources, acquisition and verification procedures, statistical properties, and temporal & geographical correlation, along with the collection process quality of the ecosystem components under study. A substantial overlapping between ecological features and human activities was identified, confirming the need for a well-planned approach to marine space management, in order to mitigate conflicts for marine resources and conserve marine ecosystems and their associated goods and services.

  1. Fabricating Hexagonal Al-Doped LiCoO2 Nanomeshes Based on Crystal-Mismatch Strategy for Ultrafast Lithium Storage.

    Xu, Hai-Tao; Zhang, Huijuan; Liu, Li; Feng, Yangyang; Wang, Yu

    2015-09-23

    In the designed synthesis, low crystal-mismatch strategy has been applied in the synthesis of ion-doped LiCoO2 materials, and a good success of single crystal property has been achieved between the precursor and the final sample for the first time. The hexagonal LiCo0.8Al0.26O2 (LCAO) nanomesh possesses several advantages in morphology and crystal structure, including mesoporous structure, single crystal, atomic even distribution, high exposing surface area as (100) or their equivalent planes, and shortened Li ions diffusion distance. All the merits are beneficial to the application in Li-ion batteries (LIBs) cathode, for example, accelerating Li ions diffusion rate, improving the Li ions shuttle between the LCAO nanomesh and electrolyte, and reducing the Li ions capacitive behavior during Li intercalation. Hence, our research adopts Al-contained precursor with morphology of hexagonal nanoplates to fabricate designed Al-doped LiCoO2 nanomeshes and greatly improves the cathode performance in LIBs. PMID:26333181

  2. Preliminary Studies on Base Substitutions and Repair of DNA Mismatch Damage Stimulated by Low Energy N+ Ion Beam Implantation in Escherichia coli

    谢传晓; 郭金华; 程备久; 余增亮

    2003-01-01

    Ever since the low energy N+ ion beam has been accepted that the mutation effectsof ionizing radiation are attributed mainly to direct or indirect damage to DNA. Evidences basedon naked DNA irradiation in support of a mutation spectrum appears to be consistent, but directproof of such results in vivo are limited. Using mutS, dam and/or dcm defective Eschericha colimutator strains, an preliminary experimental system on induction of in vivo mutation spectra oflow energy N+ ion beam has been established in this study. It was observed that the mutationrates of rifampicin resistance induced by N+ implantation were quite high, ranging from 9.2 ×10-8 to 4.9 × 10-5 at the dosage of 5.2 × 1014 ions/cm2. Strains all had more than 90-fold highermutation rate than its spontaneous mutation rate determined by this method. It reveals thatbase substitutions involve in induction of mutation of low energy nitrogen ion beam implantation.The mutation rates of mutator strains were nearly 500-fold (GM2929), 400-fold (GM5864) and6-fold larger than that of AB1157. The GM2929 and GM5864 both lose the ability of repair DNAmismatch damage by virtue of both dam and dcm pathways defective (GM2929) or failing toassemble the repair complex (GM5864) respectively. It may explain the both strains had a similarhigher mutation rate than GM124 did. It indicated that DNA cytosine methylase might play animportant role in mismatch repair of DNA damage induced by N+ implantation. The furtherrelated research were also discussed.

  3. High fitness costs of climate change-induced camouflage mismatch.

    Zimova, Marketa; Mills, L Scott; Nowak, J Joshua

    2016-03-01

    Anthropogenic climate change has created myriad stressors that threaten to cause local extinctions if wild populations fail to adapt to novel conditions. We studied individual and population-level fitness costs of a climate change-induced stressor: camouflage mismatch in seasonally colour molting species confronting decreasing snow cover duration. Based on field measurements of radiocollared snowshoe hares, we found strong selection on coat colour molt phenology, such that animals mismatched with the colour of their background experienced weekly survival decreases up to 7%. In the absence of adaptive response, we show that these mortality costs would result in strong population-level declines by the end of the century. However, natural selection acting on wide individual variation in molt phenology might enable evolutionary adaptation to camouflage mismatch. We conclude that evolutionary rescue will be critical for hares and other colour molting species to keep up with climate change. PMID:26799459

  4. Mismatch repair deficiency testing in clinical practice.

    Buza, Natalia; Ziai, James; Hui, Pei

    2016-05-01

    Lynch syndrome, an autosomal dominant inherited disorder, is caused by inactivating mutations involving DNA mismatch repair (MMR) genes. This leads to profound genetic instability, including microsatellite instability (MSI) and increased risk for cancer development, particularly colon and endometrial malignancies. Clinical testing of tumor tissues for the presence of MMR gene deficiency is standard practice in clinical oncology, with immunohistochemistry and PCR-based microsatellite instability analysis used as screening tests to identify potential Lynch syndrome families. The ultimate diagnosis of Lynch syndrome requires documentation of mutation within one of the four MMR genes (MLH1, PMS2, MSH2 and MSH6) or EPCAM, currently achieved by comprehensive sequencing analysis of germline DNA. In this review, the genetic basis of Lynch syndrome, methodologies of MMR deficiency testing, and current diagnostic algorithms in the clinical management of Lynch syndrome, are discussed. PMID:26895074

  5. Metamer mismatching in practice versus theory.

    Zhang, Xiandou; Funt, Brian; Mirzaei, Hamidreza

    2016-03-01

    Metamer mismatching (the phenomenon that two objects matching in color under one illuminant may not match under a different illuminant) potentially has important consequences for color perception. Logvinenko et al. [PLoS ONE10, e0135029 (2015)] show that in theory the extent of metamer mismatching can be very significant. This paper examines metamer mismatching in practice by computing the volumes of the empirical metamer mismatch bodies and comparing them to the volumes of the theoretical mismatch bodies. A set of more than 25 million unique reflectance spectra is assembled using datasets from several sources. For a given color signal (e.g., CIE XYZ) recorded under a given first illuminant, its empirical metamer mismatch body for a change to a second illuminant is computed as follows: the reflectances having the same color signal when lit by the first illuminant (i.e., reflect metameric light) are computationally relit by the second illuminant, and the convex hull of the resulting color signals then defines the empirical metamer mismatch body. The volume of these bodies is shown to vary systematically with Munsell value and chroma. The empirical mismatch bodies are compared to the theoretical mismatch bodies computed using the algorithm of Logvinenko et al. [IEEE Trans. Image Process.23, 34 (2014)]. There are three key findings: (1) the empirical bodies are found to be substantially smaller than the theoretical ones; (2) the sizes of both the empirical and theoretical bodies show a systematic variation with Munsell value and chroma; and (3) applied to the problem of color-signal prediction, the centroid of the empirical metamer mismatch body is shown to be a better predictor of what a given color signal might become under a specified illuminant than state-of-the-art methods. PMID:26974929

  6. A GIS-based methodology to quantitatively define an Adjacent Protected Area in a shallow karst cavity: the case of Altamira cave.

    Elez, J; Cuezva, S; Fernandez-Cortes, A; Garcia-Anton, E; Benavente, D; Cañaveras, J C; Sanchez-Moral, S

    2013-03-30

    Different types of land use are usually present in the areas adjacent to many shallow karst cavities. Over time, the increasing amount of potentially harmful matter and energy, of mainly anthropic origin or influence, that reaches the interior of a shallow karst cavity can modify the hypogeal ecosystem and increase the risk of damage to the Palaeolithic rock art often preserved within the cavity. This study proposes a new Protected Area status based on the geological processes that control these matter and energy fluxes into the Altamira cave karst system. Analysis of the geological characteristics of the shallow karst system shows that direct and lateral infiltration, internal water circulation, ventilation, gas exchange and transmission of vibrations are the processes that control these matter and energy fluxes into the cave. This study applies a comprehensive methodological approach based on Geographic Information Systems (GIS) to establish the area of influence of each transfer process. The stratigraphic and structural characteristics of the interior of the cave were determined using 3D Laser Scanning topography combined with classical field work, data gathering, cartography and a porosity-permeability analysis of host rock samples. As a result, it was possible to determine the hydrogeological behavior of the cave. In addition, by mapping and modeling the surface parameters it was possible to identify the main features restricting hydrological behavior and hence direct and lateral infiltration into the cave. These surface parameters included the shape of the drainage network and a geomorphological and structural characterization via digital terrain models. Geological and geomorphological maps and models integrated into the GIS environment defined the areas involved in gas exchange and ventilation processes. Likewise, areas that could potentially transmit vibrations directly into the cave were identified. This study shows that it is possible to define a

  7. Modeling of channel mismatch in time-interleaved SAR ADC

    Dengquan, Li; Liang, Zhang; Zhangming, Zhu; Yintang, Yang

    2015-09-01

    In a time-interleaved analog-to-digital converter (TI ADC), several individual ADCs operate in parallel to achieve a higher sampling rate. Low power consumption as well as good linearity can be obtained by applying successive approximation register (SAR) converters as sub-channel ADCs. In spite of the advantages, this structure suffers from three mismatches, which are offset mismatch, gain mismatch, and time skew. This paper focuses on a TI SAR ADC with a number of channels. The mismatch effects in the frequency domain are analyzed and the derived close form formulas are verified based on Matlab. In addition, we clarify that the standard deviation of DNL and INL of an M-channel TI ADC is reduced by a factor of \\sqrt M compared to a single channel ADC. The formulas can be used to derive the corresponding requirements when designing a TI ADC. Our analysis process is able to inform the study of calibration algorithms. Project supported by the National Natural Science Foundation of China (Nos. 61234002, 61322405, 61306044, 61376033) and the National High-Tech Program of China (No. 2013AA014103).

  8. DNA Mismatch Repair and Oxidative DNA Damage: Implications for Cancer Biology and Treatment

    Many components of the cell, including lipids, proteins and both nuclear and mitochondrial DNA, are vulnerable to deleterious modifications caused by reactive oxygen species. If not repaired, oxidative DNA damage can lead to disease-causing mutations, such as in cancer. Base excision repair and nucleotide excision repair are the two DNA repair pathways believed to orchestrate the removal of oxidative lesions. However, recent findings suggest that the mismatch repair pathway may also be important for the response to oxidative DNA damage. This is particularly relevant in cancer where mismatch repair genes are frequently mutated or epigenetically silenced. In this review we explore how the regulation of oxidative DNA damage by mismatch repair proteins may impact on carcinogenesis. We discuss recent studies that identify potential new treatments for mismatch repair deficient tumours, which exploit this non-canonical role of mismatch repair using synthetic lethal targeting

  9. Compensation of IQ mismatch in optical PDM-OFDM coherent receivers

    Chung, Hwan Seok; Chang, Sun Hyok; Kim, Kwangjoon

    2010-10-01

    The performance enhancements based on Gram-Schmidt orthogonalization procedure (GSOP) for compensating IQ mismatch in coherent optical orthogonal frequency division multiplexing (CO-OFDM) systems are investigated. We analytically explore IQ mismatch in optical OFDM systems and investigate the impacts of phase and amplitude IQ mismatch on required optical signal-to-noise ratio (OSNR) for the different values of data mapping and polarization multiplexing. The impacts of analog-to-digital converter (ADC) resolution and the number of samples in GSOP are also evaluated. The results show that the GSOP operation efficiently compensate IQ mismatch induced performance degradations regardless of the amount of IQ phase mismatch, density of data mapping, and polarization multiplexing.

  10. DNA Mismatch Repair and Oxidative DNA Damage: Implications for Cancer Biology and Treatment

    Bridge, Gemma; Rashid, Sukaina; Martin, Sarah A., E-mail: sarah.martin@qmul.ac.uk [Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ (United Kingdom)

    2014-08-05

    Many components of the cell, including lipids, proteins and both nuclear and mitochondrial DNA, are vulnerable to deleterious modifications caused by reactive oxygen species. If not repaired, oxidative DNA damage can lead to disease-causing mutations, such as in cancer. Base excision repair and nucleotide excision repair are the two DNA repair pathways believed to orchestrate the removal of oxidative lesions. However, recent findings suggest that the mismatch repair pathway may also be important for the response to oxidative DNA damage. This is particularly relevant in cancer where mismatch repair genes are frequently mutated or epigenetically silenced. In this review we explore how the regulation of oxidative DNA damage by mismatch repair proteins may impact on carcinogenesis. We discuss recent studies that identify potential new treatments for mismatch repair deficient tumours, which exploit this non-canonical role of mismatch repair using synthetic lethal targeting.

  11. Conformational arm-wrestling: battles for stereochemical control in benzamides bearing matched and mismatched chiral 2- and 6-substituents.

    Clayden, Jonathan; Foricher, Yann J Y; Helliwell, Madeleine; Johnson, Paul; Mitjans, David; Vinader, Victoria

    2006-02-01

    The orientation of a tertiary amide group adjacent to an aromatic ring may be governed by the stereochemistry of an adjacent chiral substituent. With a chiral substituent in both ortho positions, matched/mismatched pairs of isomers result. Evidence for matched stereochemistry is provided by the clean NMR spectra of single conformers, while mismatching gives poor or unexpected selectivities in the formation of chiral substituents, or mixtures of amide conformers. Attempts to use the match-mismatch effect to select for racemic pairs of enantiomeric substituents, and hence develop a "racemate-sequestering" reagent, are described, along with the use of "matching" to scavenge a single enantiomer of a diamine from material of incomplete enantiomeric purity. PMID:16446801

  12. Mismatch repair and treatment resistance in ovarian cancer

    van der Burg Maria EL; Ewing Patricia C; Ritstier Kirsten; van Kuijk Patricia F; Dinjens Winand NM; van Staveren Iris L; Helleman Jozien; Stoter Gerrit; Berns Els MJJ

    2006-01-01

    Abstract Background The treatment of ovarian cancer is hindered by intrinsic or acquired resistance to platinum-based chemotherapy. The aim of this study is to determine the frequency of mismatch repair (MMR) inactivation in ovarian cancer and its association with resistance to platinum-based chemotherapy. Methods We determined, microsatellite instability (MSI) as a marker for MMR inactivation (analysis of BAT25 and BAT26), MLH1 promoter methylation status (methylation specific PCR on bisulfi...

  13. SKILLS MISMATCH OF THE YOUNG PEOPLE AT THE EUROPEAN LEVEL

    Hatos Roxana

    2015-07-01

    Full Text Available Transition from school to work is a main issue with many fields of study. Studies on transition from school to work, have highlight the importance of two categories of factors at the level of the individual formal proceedings which may affect how easy it is to graduate to integrate into the labor market: 1 so far as the educational systems are transmitting specific competences as compared with those general and 2 so far as there are direct links between employers and the education system. In this way, are reduced the costs of selection and allocation for employers. A poor articulation between educational institutions and the labor market produce a high level of unmatched competences of assimilated by formal education and competencies required of the labor market (skill mismatch (Parodi et al., 2012. The surveys with European employers reflect particular difficulties that they are experiencing in employment vacancies. Investigation on the European companies in the spring of 2013 found that 40% of the firms in the EU have difficulty in finding employees with suitable qualification (CEDEFOP-European Center for the Development of the Vocational Training, 2014. Skills mismatch is a generic term that refers to various types of imbalances between skills and competences offered and those required in the labor market. Concept has become one intensely discussed and submitted to measurement in international research on the background concerns the under-utilization human resource. Numerous opinion polls with employers come to the same unexpected conclusion - that despite high unemployment many posts can't find occupants satisfactorily prepared and identify the causes: most of them criticized the lack of skills of the candidates or the absence of skills specific to the workplace. Based on the latest studies on international databases have built a set of questions that, through secondary analysis, we tried to find answers. Questions that we try to give answer

  14. Mismatch Calibration in LINC Power Amplifiers Using Modified Gradient Algorithm

    Hosein Miar-Naimi

    2013-09-01

    Full Text Available One of the power amplifiers linearization technique is linear amplification with nonlinear components (LINC.The effects of phase and gain imbalances between two signal branches in LINC transmitters have been analyzed in this paper. Then a feedback path has been added to compensate this mismatches, using two complex gain in each path.This complex gains are controlled in a way to calibrate any gain and phase mismatches between two path using Modified Gradient Algorithm (MGA adaptively. The main advantages of this algorithm over other algorithms are zero residual error and fast convergence time. In the proposedarchitecture power amplifiers in each path are modeled as a complex gain which its phase and amplitude depend on input signal level. Many simulations have been performed to validate the proposed self calibrating LINC transmitter. Simulation results have confirmed the analyticalpredictions. According to simulation results the proposed structure has around 40 dB/Hz improvement in the first adjacent channel of the output signal spectrum.

  15. Improved Dijkstra Algorithm Based on the Adjacent Point in Shortest Path%基于邻接点算法的Dijkstra优化研究

    王华

    2013-01-01

    Abstract the urban road into network diagram and definition of data structures for road and site. Improve the Dijkstra by adjacent point algorithm, the method build the adjacency matrix and the initial judgment matrix and get the shortest path between two points in the network diagram. The efficiency of the algorithm is apparent when there are more points. Experiments show that adjacent point algorithm not only saves storage space, but also improves the efficiency of operations.%在将城市道路实体抽象为图论理论中的网络图,对道路和站点数据结构进行定义的基础上,利用邻接点算法对Dijkstra算法进行改进,通过构建邻接矩阵和初始判断矩阵,得出网络图中任意两点间的最短路径.当点数越多时改进算法的效率就显现出来,结果表明邻接点算法在一定程度上既节省了存储空间,又提高了运算效率.

  16. Wavelength mismatch effect in electromagnetically induced absorption

    Bharti, Vineet; Wasan, Ajay; Natarajan, Vasant

    2016-01-01

    We present a theoretical investigation of the phenomenon of electromagnetically induced absorption (EIA) in a 4-level system consisting of vee and ladder subsystems. The four levels are coupled using one weak probe field, and two strong control fields. We consider an experimental realization using energy levels of Rb. This necessitates dealing with different conditions of wavelength mismatch---near-perfect match where all three wavelengths are approximately equal; partial mismatch where the w...

  17. Migrant Educational Mismatch and the Labour Market

    Piracha, Matloob; Vadean, Florin

    2012-01-01

    This paper reviews the literature on educational mismatch of immigrants in the labour market of destination countries. It draws on the theoretical arguments postulated in the labour economics literature and discusses their extension in the analysis of the causes and effects of immigrants' educational mismatch in the destination country. Relevant empirical approaches have been presented which show that immigrants are in general more over-educated than natives and that the reasons for those ran...

  18. Ventromedial prefrontal cortex drives hippocampal theta oscillations induced by mismatch computations.

    Garrido, Marta I; Barnes, Gareth R; Kumaran, Dharshan; Maguire, Eleanor A; Dolan, Raymond J

    2015-10-15

    Detecting environmental change is fundamental for adaptive behavior in an uncertain world. Previous work indicates the hippocampus supports the generation of novelty signals via implementation of a match-mismatch detector that signals when an incoming sensory input violates expectations based on past experience. While existing work has emphasized the particular contribution of the hippocampus, here we ask which other brain structures also contribute to match-mismatch detection. Furthermore, we leverage the fine-grained temporal resolution of magnetoencephalography (MEG) to investigate whether mismatch computations are spectrally confined to the theta range, based on the prominence of this range of oscillations in models of hippocampal function. By recording MEG activity while human subjects perform a task that incorporates conditions of match-mismatch novelty we show that mismatch signals are confined to the theta band and are expressed in both the hippocampus and ventromedial prefrontal cortex (vmPFC). Effective connectivity analyses (dynamic causal modeling) show that the hippocampus and vmPFC work as a functional circuit during mismatch detection. Surprisingly, our results suggest that the vmPFC drives the hippocampus during the generation and processing of mismatch signals. Our findings provide new evidence that the hippocampal-vmPFC circuit is engaged during novelty processing, which has implications for emerging theories regarding the role of vmPFC in memory. PMID:26187453

  19. Asymmetric response of demand-supply mismatch to investor's sentiment

    Marcato, Gianluca; Nanda, Anupam

    2012-01-01

    We look through both the demand and supply side information to understand dynamics of price determination in the real estate market and examine how accurately investors’ attitudes predict the market returns and thereby flagging off extent of any demand-supply mismatch. Our hypothesis is based on the possibility that investors’ call for action in terms of their buy/sell decision and adjustment in reservation/offer prices may indicate impending demand-supply imbalances in the market. In the pro...

  20. SKILL MISMATCH OF GRADUATES IN A LOCAL LABOUR MARKET

    Enrico Marelli; Dario Sciulli; Marcello Signorelli

    2014-01-01

    In this paper we first review the (potential and actual) role of the Universities for the local economies in which they operate, especially considering the implications deriving from the degree of skill mismatch (over-education) in a local labour market. Then, in the second part of the paper, we realise an empirical investigation based on administrative information of an Italian University matched with the data of the job centres of the local (provincial) labour market in order to...

  1. Nonspinning searches for spinning binaries in ground-based detector data: Amplitude and mismatch predictions in the constant precession cone approximation

    Brown, D; O'Shaughnessy, R

    2012-01-01

    Current searches for compact binary mergers by ground-based gravitational-wave detectors assume for simplicity the two bodies are not spinning. If the binary contains compact objects with significant spin, then this can reduce the sensitivity of these searches, particularly for black hole--neutron star binaries. In this paper we investigate the effect of neglecting precession on the sensitivity of searches for spinning binaries using non-spinning waveform models. We demonstrate that in the sensitive band of Advanced LIGO, the angle between the binary's orbital angular momentum and its total angular momentum is approximately constant. Under this \\emph{constant precession cone} approximation, we show that the gravitational-wave phasing is modulated in two ways: a secular increase of the gravitational-wave phase due to precession and an oscillation around this secular increase. We show that this secular evolution occurs in precisely three ways, corresponding to physically different apparent evolutions of the bin...

  2. In Situ Measurement of the γ/ γ' Lattice Mismatch Evolution of a Nickel-Based Single-Crystal Superalloy During Non-isothermal Very High-Temperature Creep Experiments

    Le Graverend, Jean-Briac; Dirand, Laura; Jacques, Alain; Cormier, Jonathan; Ferry, Olivier; Schenk, Thomas; Gallerneau, Franck; Kruch, Serge; Mendez, José

    2012-11-01

    The evolution of the γ/ γ' lattice mismatch of the AM1 single-crystal superalloy was measured during in situ non-isothermal very high-temperature creep tests under X-ray synchrotron radiation. The magnitude of the effective lattice mismatch in the 1273 K to 1323 K (1000 °C to 1050 °C) temperature range always increased after overheatings performed at temperatures lower than 1403 K (1130 °C). In contrast, a decrease of its magnitude was observed after overheatings at temperatures greater than 1453 K (1180 °C) due to massive dislocation recovery processes occurring at very high temperature.

  3. Fuzzy Backstepping Sliding Mode Control for Mismatched Uncertain System

    H. Q. Hou

    2014-06-01

    Full Text Available Sliding mode controllers have succeeded in many control problems that the conventional control theories have difficulties to deal with; however it is practically impossible to achieve high-speed switching control. Therefore, in this paper an adaptive fuzzy backstepping sliding mode control scheme is derived for mismatched uncertain systems. Firstly fuzzy sliding mode controller is designed using backstepping method based on the Lyapunov function approach, which is capable of handling mismatched problem. Then fuzzy sliding mode controller is designed using T-S fuzzy model method, it can improve the performance of the control systems and their robustness. Finally this method of control is applied to nonlinear system as a case study; simulation results are also provided the performance of the proposed controller.

  4. Wavelength mismatch effect in electromagnetically induced absorption

    Bharti, Vineet; Wasan, Ajay; Natarajan, Vasant

    2016-07-01

    We present a theoretical investigation of the phenomenon of electromagnetically induced absorption (EIA) in a 4-level system consisting of vee and ladder subsystems. The four levels are coupled using one weak probe field, and two strong control fields. We consider an experimental realization using energy levels of Rb. This necessitates dealing with different conditions of wavelength mismatch-near-perfect match where all three wavelengths are approximately equal; partial mismatch where the wavelength of one control field is less than the other fields; and complete mismatch where all three wavelengths are unequal. We present probe absorption profiles with Doppler averaging at room temperature to account for experiments in a room temperature Rb vapor cell. Our analysis shows that EIA resonances can be studied using Rydberg states excited with diode lasers.

  5. Wavelength mismatch effect in electromagnetically induced absorption

    Bharti, Vineet; Natarajan, Vasant

    2016-01-01

    We present a theoretical investigation of the phenomenon of electromagnetically induced absorption (EIA) in a 4-level system consisting of vee and ladder subsystems. The four levels are coupled using one weak probe field, and two strong control fields. We consider an experimental realization using energy levels of Rb. This necessitates dealing with different conditions of wavelength mismatch---near-perfect match where all three wavelengths are approximately equal; partial mismatch where the wavelength of one control field is less than the other fields; and complete mismatch where all three wavelengths are unequal. We present probe absorption profiles with Doppler averaging at room temperature to account for experiments in a room temperature Rb vapor cell. Our analysis shows that EIA resonances can be studied using Rydberg states excited with diode lasers.

  6. Mismatch and noise in modern IC processes

    Marshall, Andrew

    2009-01-01

    Component variability, mismatch, and various noise effects are major contributors to design limitations in most modern IC processes. Mismatch and Noise in Modern IC Processes examines these related effects and how they affect the building block circuits of modern integrated circuits, from the perspective of a circuit designer.Variability usually refers to a large scale variation that can occur on a wafer to wafer and lot to lot basis, and over long distances on a wafer. This phenomenon is well understood and the effects of variability are included in most integrated circuit design with the use

  7. A new interpretation of deformation rates in the Snake River Plain and adjacent basin and range regions based on GPS measurements

    S.J. Payne; R. McCaffrey; R.W. King; S.A. Kattenhorn

    2012-04-01

    We evaluate horizontal Global Positioning System (GPS) velocities together with geologic, volcanic, and seismic data to interpret extension, shear, and contraction within the Snake River Plain and the Northern Basin and Range Province, U.S.A. We estimate horizontal surface velocities using GPS data collected at 385 sites from 1994 to 2009 and present an updated velocity field within the Stable North American Reference Frame (SNARF). Our results show an ENE-oriented extensional strain rate of 5.9 {+-} 0.7 x 10{sup -9} yr{sup -1} in the Centennial Tectonic belt and an E-oriented extensional strain rate of 6.2 {+-} 0.3 x 10{sup -9} yr{sup -1} in the Intermountain Seismic belt combined with the northern Great Basin. These extensional strain rates contrast with the regional north-south contraction of -2.6 {+-} 1.1 x 10{sup -9} yr{sup -1} calculated in the Snake River Plain and Owyhee-Oregon Plateau over a 125 x 650 km region. Tests that include dike-opening reveal that rapid extension by dike intrusion in volcanic rift zones does not occur in the Snake River Plain at present. This slow internal deformation in the Snake River Plain is in contrast to the rapidly-extending adjacent Basin and Range provinces and implies shear along boundaries of the Snake River Plain. We estimate right-lateral shear with slip rates of 0.5-1.5 mm/yr along the northwestern boundary adjacent to the Centennial Tectonic belt and left-lateral oblique extension with slip rates of <0.5 to 1.7 mm/yr along the southeastern boundary adjacent to the Intermountain Seismic belt. The fastest lateral shearing occurs near the Yellowstone Plateau where strike-slip focal mechanisms and faults with observed strike-slip components of motion are documented. The regional GPS velocity gradients are best fit by nearby poles of rotation for the Centennial Tectonic belt, Idaho batholith, Snake River Plain, Owyhee-Oregon Plateau, and central Oregon, indicating that clockwise rotation is driven by extension to the

  8. Nonequilibrium phenomena in adjacent electrically isolated nanostructures

    Khrapai, V. S.; Ludwig, S.; Kotthaus, J. P.; Tranitz, H. P.; Wegscheider, W.

    2008-01-01

    We report on nonequilibrium interaction phenomena between adjacent but electrostatically separated nanostructures in GaAs. A current flowing in one externally biased nanostructure causes an excitation of electrons in a circuit of a second nanostructure. As a result we observe a dc current generated in the unbiased second nanostructure. The results can be qualitatively explained in terms of acoustic phonon based energy transfer between the two mutually isolated circuits.

  9. New Spectral Method for Halo Particle Definition in Intense Mis-matched Beams

    An advanced spectral analysis of a mis-matched charged particle beam propagating through a periodic focusing transport lattice is utilized in particle-in-cell (PIC) simulations. It is found that the betatron frequency distribution function of a mismatched space-charge-dominated beam has a bump-on-tail structure attributed to the beam halo particles. Based on this observation, a new spectral method for halo particle definition is proposed that provides the opportunity to carry out a quantitative analysis of halo particle production by a beam mismatch. In addition, it is shown that the spectral analysis of the mismatch relaxation process provides important insights into the emittance growth attributed to the halo formation and the core relaxation processes. Finally, the spectral method is applied to the problem of space-charge transport limits.

  10. Analysis of the injection-locked magnetron with a mismatched circulator

    Based on the scatter matrix of the four-port lossless mismatched circulator, the phase differential equation of the injection-locked magnetron is derived by comparing different effects of the mismatched and perfect circulator on the injection ratio. Besides, the locking range of the injection-locked magnetron with the mismatched circulator is deduced by functional operation. In addition, the phase differential equation and the locked bandwidth of the injection-locked system with a mismatched circulator are compared with those of the small injection-ratio case with a perfect circulator. The influence of the circulator reflection coefficient on the injection-locked magnetron is also analyzed by numerical calculation. Theoretical analysis shows that the decrement of the locked bandwidth is less than 1% and decrement of the stable phase difference is less than 1.2% when the reflection coefficient is less than 0.1. (interdisciplinary physics and related areas of science and technology)

  11. Velocity synchronization of multi-agent systems with mismatched parameters via sampled position data

    Sun, Wen; Huang, Chunli; Lü, Jinhu; Li, Xiong; Chen, Shihua

    2016-02-01

    Power systems are special multi-agent systems with nonlinear coupling function and symmetric structures. This paper extends these systems to a class of multi-agent systems with mismatched parameters, linear coupling function, and asymmetric structures and investigates their velocity synchronization via sampled position data. The dynamics of the agents is adopted as that of generators with mismatched parameters, while the system structures are supposed to be complex. Two distributed linear consensus protocols are designed, respectively, for multi-agent systems without or with communication delay. Necessary and sufficient conditions based on the sampling period, the mismatched parameters, the delay, and the nonzero eigenvalues of the Laplacian matrix are established. It is shown that velocity synchronization of multi-agent systems with mismatched parameters can be achieved if the sampled period is chosen appropriately. Simulations are given to illustrate the effectiveness of the theoretical results.

  12. Calibration of P/S amplitude ratios for seismic events in Xinjiang and its adjacent areas based on a Bayesian Kriging method

    PAN Chang-zhou; JIN Ping; XIAO Wei-guo

    2007-01-01

    Correction maps of P/S amplitude ratios for seismic events distributed in Xinjiang, China and its adjacent areas were established using a Bayesian Kriging method for the two seismic stations WMQ and MAK. The relationship between correction maps and variations of along-path features was analyzed and the validity of applying the correction maps to improve performances of P/S discriminants for seismic discrimination was investigated. Results show that obtained correction maps can generally reflect event-station path effects upon corresponding P/S discriminants; and the correction of these effects could further reduce scatters of distance-corrected P/S measurements within earthquake and explosion populations as well as improve their discriminating performances if path effects are a significant factor of such scatters. For example, as corresponding Kriging correction map was applied, the misidentification rate of earthquakes by Pn(2~4 Hz)/Lg(2~4 Hz) at MAK was reduced from 16.3% to 5.2%.

  13. Circuit mismatch influence on performance of paralleling silicon carbide MOSFETs

    Li, Helong; Munk-Nielsen, Stig; Pham, Cam;

    2014-01-01

    This paper focuses on circuit mismatch influence on performance of paralleling SiC MOSFETs. Power circuit mismatch and gate driver mismatch influences are analyzed in detail. Simulation and experiment results show the influence of circuit mismatch and verify the analysis. This paper aims to give...... suggestions on paralleling discrete SiC MOSFETs and designing layout of power modules with paralleled SiC MOSFETs dies....

  14. Dynamics of beam halo in mismatched beams

    High-power proton linacs for nuclear materials transmutation and production, and new accelerator-driven neutron spallation sources must be designed to control beam-halo formation, which leads to beam loss. The study of particle-core models is leading to a better understanding of the causes and characteristics of beam halo produced by space-charge forces in rms mismatched beams. Detailed studies of the models have resulted in predictions of the dependence of the maximum amplitude of halo particles on a mismatch parameter and on the space-charge tune-depression ratio. Scaling formulas have been derived which will provide guidance for choosing the aperture radius to contain the halo without loss. (author)

  15. Education, Social Mobility, and Talent Mismatch

    Yuki Uchida

    2015-01-01

    This study presents a two-class, overlapping-generation model featuring social mobility inhibited by the mismatch of talents. Mobility decreases as the private education gap between the two classes widens, whereas it increases with an increased public education spending. Within this framework, we consider the redistributive politics of public education and show that the private education gap provides the government with an incentive to increase public education. We also show that social mobil...

  16. Social Networks and the Labour Market Mismatch

    Kalfa, Eleni; Piracha, Matloob

    2015-01-01

    This paper assesses the extent to which social contacts and ethnic concentration affect the education-occupation mismatch of natives and immigrants. Using Australian panel data and employing a dynamic random effects probit model, we show that social capital exacerbates the incidence of over-education, particularly for females. Furthermore, for the foreign-born, ethnic concentration significantly increases the incidence of over-education. Using an alternative index, we also show that social pa...

  17. 基于邻接矩阵的软件测试环境规划设计方法%Design Method of Software Test Environment Based on Adjacency Matrix

    张晓娜; 金朋飞; 严长伟

    2016-01-01

    在分析软件测试环境的基础上,研究了软件试验中测试用例设计面临的突出问题,从软件测试环境的规划出发,基于有向图的邻接矩阵,设计满足一定覆盖度标准的软件测试环境规划方法,可为软件试验提供技术参考和研究方向。%Based on the analysis about the software test environment, this paper studies the key problems of the test case de⁃sign in the software test, based on the adjacency matrix of directed graph, designs the software test environment plan method up to coverage criteria. All these researches can provide technology reference for the future development of software test.

  18. Ecologic and geographic distributions of the vascular plants of southern Nye County, and adjacent parts of Clark, Lincoln, and Esmeralda Counties, Nevada. [Based on collections made in 1970

    Beatley, J. C.

    1971-01-01

    A catalog is compiled of the vascular plants indiginous to Nye, Clark, Lincoln, and Esmeralda Counties of Nevada based on collections made in 1970. This compilation is an update of previous collections in these areas and is a supplement to report, UCLA--12-705. (ERB)

  19. Kinetics and binding of the thymine-DNA mismatch glycosylase, Mig-Mth, with mismatch-containing DNA substrates.

    Begley, Thomas J; Haas, Brian J; Morales, Juan C; Kool, Eric T; Cunningham, Richard P

    2003-01-01

    We have examined the removal of thymine residues from T-G mismatches in DNA by the thymine-DNA mismatch glycosylase from Methanobacterium thermoautrophicum (Mig-Mth), within the context of the base excision repair (BER) pathway, to investigate why this glycosylase has such low activity in vitro. Using single-turnover kinetics and steady-state kinetics, we calculated the catalytic and product dissociation rate constants for Mig-Mth, and determined that Mig-Mth is inhibited by product apyrimidinic (AP) sites in DNA. Electrophoretic mobility shift assays (EMSA) provide evidence that the specificity of product binding is dependent upon the base opposite the AP site. The binding of Mig-Mth to DNA containing the non-cleavable substrate analogue difluorotoluene (F) was also analyzed to determine the effect of the opposite base on Mig-Mth binding specificity for substrate-like duplex DNA. The results of these experiments support the idea that opposite strand interactions play roles in determining substrate specificity. Endonuclease IV, which cleaves AP sites in the next step of the BER pathway, was used to analyze the effect of product removal on the overall rate of thymine hydrolysis by Mig-Mth. Our results support the hypothesis that endonuclease IV increases the apparent activity of Mig-Mth significantly under steady-state conditions by preventing reassociation of enzyme to product. PMID:12509271

  20. Methodical approaches to the implementation of radiation-ecological and sanitary investigation of the areas of the former military bases and recreation zones adjacent to them

    According to the Decree of the Council of Ministers of the Republic of Belarus N856 dated 21.12.93, the possibility of using the former military bases for remediation of the population from the Chernobyl accident affected areas has been investigated. It was necessary to conduct a detailed investigation of the territory, as there was no information on sanitary and radioecological state on the sites. Firstly, contamination level and contamination areals were to be defined, as well as evaluation of danger risk for the population. Secondly, the obtained results of the investigation should form the basis for further estimation of the environment state on the sites, as well as for decision making on the territory remediation

  1. 基于粗糙集的相邻交叉口信号控制方法研究%Adjacent Intersection Signal Control Based on Rough Set

    李璐兵; 陈荣升

    2014-01-01

    复杂交通区域的交通状况难以利用具体模型直接描述,而粗糙集理论能够在分析大量经验数据的基础上找到用于决策的规则。以交大东路上的行人和机动车为研究对象,提出一种基于粗糙集的信号配时算法,对交大东路上的两个相邻交叉口的信号灯进行控制,提出控制规则,对该复杂交通区域进行优化和疏导,是对多交叉口交通管理与控制的一次有益尝试。%It′s difficult to accurately describe the traffic condition in complex traffic areas using specific formulas. But by analyzing plenty of data based on rough set theory, rules can be found for decision-mak-ing. The article put pedestrians and vehicles on Jiaoda-East Road as the research object, and then pre-sented with a signal algorithm based on rough set to control two neighboring traffic lights in the road and reduce traffic pressure in this complex traffic area. Finally, it proposed control rules and guidance to opti-mize the complex traffic area, which was a beneficial trial for multiple intersections group on traffic man-agement and control.

  2. Visual mismatch negativity: A predictive coding view

    Gabor eStefanics

    2014-09-01

    Full Text Available An increasing number of studies investigate the visual mismatch negativity (vMMN or use the vMMN as a tool to probe various aspects of human cognition. This paper reviews the theoretical underpinnings of vMMN in the light of methodological considerations and provides recommendations for measuring and interpreting the vMMN. The following key issues are discussed from the experimentalist’s point of view in a predictive coding framework: 1 experimental protocols and procedures to control ‘refractoriness’ effects; 2 methods to control attention; 3 vMMN and veridical perception.

  3. Barbed Congruence of Asymmetry and Mismatch

    Xiao-Ju Dong; Yu-Xi Fu

    2007-01-01

    The X calculus is a model of concurrent and mobile systems. It emphasizes that communications are information exchanges. In the paper, two constructions are incorporated into the framework of the chi calculus, which are asymmetric communication and mismatch condition widely used in applications. Since the barbed bisimilarity has proved its generality and gained its popularity as an effective approach to generating a reasonable observational equivalence, we study both the operational and algebraic properties of the barbed bisimilarity in this enriched calculus. The investigation supports an improved understanding of the bisimulation behaviors of the model. It also gives a general picture of how the two constructions affect the observational theory.

  4. Kauffman's adjacent possible in word order evolution

    Ferrer-i-Cancho, Ramon

    2015-01-01

    Word order evolution has been hypothesized to be constrained by a word order permutation ring: transitions involving orders that are closer in the permutation ring are more likely. The hypothesis can be seen as a particular case of Kauffman's adjacent possible in word order evolution. Here we consider the problem of the association of the six possible orders of S, V and O to yield a couple of primary alternating orders as a window to word order evolution. We evaluate the suitability of various competing hypotheses to predict one member of the couple from the other with the help of information theoretic model selection. Our ensemble of models includes a six-way model that is based on the word order permutation ring (Kauffman's adjacent possible) and another model based on the dual two-way of standard typology, that reduces word order to basic orders preferences (e.g., a preference for SV over VS and another for SO over OS). Our analysis indicates that the permutation ring yields the best model when favoring pa...

  5. What Do Educational Mismatches Tell Us About Skill Mismatches? A Cross-country Analysis

    Allen, Jim; Weert, de Egbert

    2007-01-01

    The relationship between higher education and employment is commonly interpreted in terms of the extent to which the higher education sector is providing graduates with the knowledge and skills to match employment needs. It is assumed that a mismatch between working in a job and level or field of ed

  6. How relevant is job mismatch for German graduates?

    Berlingieri, Francesco; Erdsiek, Daniel

    2012-01-01

    In this study, we examine the incidence and direct consequences of job mismatch for German graduates. Beyond measuring job mismatch by the comparison of qualification obtained by employees and required for a job, we employ self-reported skill mismatch variables concerning overall skills and more detailed information about the surplus or deficit in specific competences. The results indicate that a substantial share of graduates underutilizes own skills in the job. The rate of overqualification...

  7. A Model Driven approach for Applicationspecific Mismatch Detection

    Amit Raj

    2013-01-01

    Several large-scale systems are developed in modules that are assembled together to deliver a desired functionality. The modules developed in isolation may not implement the desired requirements. Itcreates several problems either at design-time, deployment-time or at run-time. Existing research defines a class of such problems as mismatches. A mismatch may cause malfunctioning or crash of a system.Therefore, a mismatch needs to be detected and resolved. The paper describes a model-driven gene...

  8. Quantum noise in parametric amplification under phase-mismatched conditions

    Inoue, K.

    2016-05-01

    This paper studies quantum noise in parametric amplification under phase-mismatched conditions. The equations of motion of the quantum-mechanical field operators, which include phase mismatch under unsaturated conditions are first derived from the Heisenberg equation. Next, the noise figure is evaluated using the solutions of the derived equations. The results indicate that phase mismatch scarcely affects noise property in phase-insensitive amplification while it has a notable effect in case of phase-sensitive amplification.

  9. An IQ mismatch calibration and compensation technique for wideband wireless transceivers

    Jin, Peng; Liguo, Zhou; Heng, Yao; Fang, Yuan; Zhi, Fang; Yin, Shi

    2014-08-01

    An IQ mismatch calibration and compensation technique based on the digital baseband for wideband wireless communication transmitters is proposed. The digital baseband transmits the signal used for IQ mismatch calibration. The signal passes through the RF transmitter path, the calibration loop (which is composed of a square power detector and a band-pass filter in the RF transceiver) and the variable gain amplifier of the receiver. The digital baseband samples the signal for IQ mismatch estimation and compensates for it. Compared with the self-calibration technique in the RF chip, the proposed technique saves area and power consumption for the wireless local area network solution. This technique has been successfully used for the 802.11n system and satisfies the requirement of the standard by achieving over 50 dB image suppression.

  10. An IQ mismatch calibration and compensation technique for wideband wireless transceivers

    An IQ mismatch calibration and compensation technique based on the digital baseband for wideband wireless communication transmitters is proposed. The digital baseband transmits the signal used for IQ mismatch calibration. The signal passes through the RF transmitter path, the calibration loop (which is composed of a square power detector and a band-pass filter in the RF transceiver) and the variable gain amplifier of the receiver. The digital baseband samples the signal for IQ mismatch estimation and compensates for it. Compared with the self-calibration technique in the RF chip, the proposed technique saves area and power consumption for the wireless local area network solution. This technique has been successfully used for the 802.11n system and satisfies the requirement of the standard by achieving over 50 dB image suppression. (semiconductor integrated circuits)

  11. Labour Market Mismatch among UK Graduates: An Analysis Using REFLEX Data

    McGuinness, Seamus; Sloane, Peter J.

    2011-01-01

    There is much disagreement in the literature over the extent to which graduates are mismatched in the labour market and the reasons for this. In this paper we utilise the Flexible Professional in the Knowledge Society (REFLEX) data set to cast light on these issues, based on data for UK graduates. We find substantial pay penalties for…

  12. The mouse mismatch repair protein, MSH3, is a nucleoplasmic protein that aggregates into denser nuclear bodies under conditions of stress.

    Holt, I.; Thanh Lam, L.; Tome, S.; Wansink, D.G.; Riele, H. te; Gourdon, G.; Morris, G.E.

    2011-01-01

    The mismatch repair protein, MSH3, together with MSH2, forms the MutSbeta heterodimer which recognizes and repairs base pair mismatches and larger insertion/deletion loops in DNA. Lack of specific antibodies against mouse MSH3 has hampered studies of its expression and localization. Mouse MSH3 is no

  13. Local approach to strength mis-match effect on cleavage fracture of notched material

    This paper discusses the strength mis-match effect on cleavage fracture of notched materials based on the local approach. The aim of the study is to verify the independence of the critical Weibull stress at fracture on the strength mis-match condition. Diffusion bonded joints were made with two materials S and H different in strength level. Two types of 3-point bend specimen were extracted. One had a notch in the lower strength material S near the bonded interface, and another in the higher strength material H. Homogeneous specimens were also made for each material. The critical CTOD value at cleavage fracture for the overmatched specimen with a notch in the material S was smaller than that of the homogeneous specimen of the material S. By contrast, the undermatched specimen with a notch in the material H showed apparently larger critical CTOD value than the homogeneous specimen of the material H. The strength mis-match effect on the CTOD results was explained in terms of the constraint effect on the near-tip stress field. Namely, the near-tip stress is elevated by strength overmatching and relaxed by strength undermatching. The Weibull stresses for the homogeneous and mis-matched specimens were evaluated in the light of the near-tip stress fields. The critical Weibull stress at fracture did not depend on the strength mis-match condition near the notch. By using the critical Weibull stress obtained for the homogeneous specimen, the strength mis-match effect on the CTOD results can be predicted. Close agreement was observed between the critical CTOD value predicted and that obtained by the experiment. (orig.)

  14. Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene

    Mutations affecting heteroduplex DNA mismatch repair in Streptococcus pneumoniae were localized in two genes, hexA and hexB, by fractionation of restriction fragments carrying mutant alleles. A fragment containing the hexA4 allele was cloned in the S. pneumoniae cloning system, and the hexA+ allele was introduced into the recombinant plasmid by chromosomal facilitation of plasmid transfer. Subcloning localized the functional hexA gene to a 3.5-kilobase segment of the cloned pneumococcal DNA. The product of this gene was shown in Bacillus subtilis minicells to be a polypeptide with an M/sub r/ of 86,000. Two mutant alleles of hexA showed partial expression of the repair system when present in multicopy plasmids. A model for mismatch repair, which depends on the interaction of two protein components to recognize the mismatched base pair and excise a segment of DNA between strand breaks surrounding the mismatch, is proposed

  15. Supply-demand mismatch transients in susceptible peri-infarct hot zones explain the origin of spreading injury depolarizations

    von Bornstädt, Daniel; Houben, Thijs; Seidel, Jessica; Zheng, Yi; Dilekoz, Ergin; Qin, Tao; Sandow, Nora; Kura, Sreekanth; Eikermann-Haerter, Katharina; Endres, Matthias; Boas, David A.; Moskowitz, Michael A.; Lo, Eng H.; Dreier, Jens P.; Woitzik, Johannes; Sakadžić, Sava; Ayata, Cenk

    2015-01-01

    SUMMARY Peri-infarct depolarizations (PIDs) are seemingly spontaneous spreading depression-like waves that negatively impact tissue outcome in both experimental and human stroke. Factors triggering PIDs are unknown. Here, we show that somatosensory activation of peri-infarct cortex triggers PIDs when the activated cortex is within a critical range of ischemia. We show that the mechanism involves increased oxygen utilization within the activated cortex, worsening the supply-demand mismatch. We support the concept by clinical data showing that mismatch predisposes to PIDs in human stroke as well. Conversely, transient worsening of mismatch by episodic hypoxemia or hypotension also reproducibly triggers PIDs. Therefore, PIDs are triggered upon supply-demand mismatch transients in metastable peri-infarct hot zones due to increased demand or reduced supply. Based on the data, we propose that minimizing sensory stimulation and hypoxic or hypotensive transients in stroke and brain injury would reduce PID incidence and their adverse impact on outcome. PMID:25741731

  16. Skill Mismatch of Graduates in a Local Labour Market

    Enrico Marelli

    2014-06-01

    Full Text Available In this paper we first review the (potential and actual role of the Universities for the local economies in which they operate, especially considering the implications deriving from the degree of skill mismatch (over-education in a local labour market. Then, in the second part of the paper, we realise an empirical investigation based on administrative information of an Italian University matched with the data of the job centres of the local (provincial labour market in order to reconstruct the characteristics of the university-to-work transitions of graduates. Our results have important policy implications, since for local development it is crucial, among other things, to make the best use of all human resources and especially those with the highest educational level.

  17. Mismatch-Shaping Serial Digital-to-Analog Converter

    Steensgaard-Madsen, Jesper; Moon, Un-Ku; Temes, Gabor C.

    1999-01-01

    A simple but accurate pseudo-passive mismatch-shaping D/A converter is described. A digital state machine is used to control the switching sequence of a symmetric two-capacitor network that performs the D/A conversion. The error caused by capacitor mismatch is uncorrelated with the input signal a...

  18. Mismatch-Shaped Pseudo-Passive Two-Capacitor DAC

    Steensgaard-Madsen, Jesper; Moon, Un-Ku; Temes, Gabor C.

    1999-01-01

    A simple mismatch-shaping scheme is proposed for a two-capacitor DAC. Unlike in other mismatch-shaping systems, the shaped error is generated by direct filtering of a well-defined bounded signal, which can be generated as white noise. The operation is closely related to a specific digital interpo...

  19. The mismatch between job openings and job seekers

    Canon, Maria E.; Mingyu Chen

    2011-01-01

    Today's high unemployment rate is often linked to a structural imbalance—a mismatch between the skills and location required to fill vacant jobs and the skills and geographical preferences of the unemployed. But the evidence downplays the role of this mismatch.

  20. Improved DNA clamps by stacking to adjacent nucleobases

    Fatthalla, M.I.; Pedersen, Erik Bjerregaard

    2012-01-01

    Three or four aromatic rings interconnected by acetylene bridges form a stiff conjugated system with sufficient conformational freedom to make it useful to link together the two strands of a DNA clamp. Upon targeting a ssDNA, the conformational flexibility allows better stacking of the linker to...... the underlying non-planar base triplet in the formed triplex. This type of triplexes has a substantially higher thermal melting temperature which can be further improved by inserting locked nucleic acids (LNAs) in the Hoogsteen part of the clamp. An extremely high sensitivity to mismatches is observed...

  1. Reducing measurement scale mismatch to improve surface energy flux estimation

    Iwema, Joost; Rosolem, Rafael; Rahman, Mostaquimur; Blyth, Eleanor; Wagener, Thorsten

    2016-04-01

    Soil moisture importantly controls land surface processes such as energy and water partitioning. A good understanding of these controls is needed especially when recognizing the challenges in providing accurate hyper-resolution hydrometeorological simulations at sub-kilometre scales. Soil moisture controlling factors can, however, differ at distinct scales. In addition, some parameters in land surface models are still often prescribed based on observations obtained at another scale not necessarily employed by such models (e.g., soil properties obtained from lab samples used in regional simulations). To minimize such effects, parameters can be constrained with local data from Eddy-Covariance (EC) towers (i.e., latent and sensible heat fluxes) and Point Scale (PS) soil moisture observations (e.g., TDR). However, measurement scales represented by EC and PS still differ substantially. Here we use the fact that Cosmic-Ray Neutron Sensors (CRNS) estimate soil moisture at horizontal footprint similar to that of EC fluxes to help answer the following question: Does reduced observation scale mismatch yield better soil moisture - surface fluxes representation in land surface models? To answer this question we analysed soil moisture and surface fluxes measurements from twelve COSMOS-Ameriflux sites in the USA characterized by distinct climate, soils and vegetation types. We calibrated model parameters of the Joint UK Land Environment Simulator (JULES) against PS and CRNS soil moisture data, respectively. We analysed the improvement in soil moisture estimation compared to uncalibrated model simulations and then evaluated the degree of improvement in surface fluxes before and after calibration experiments. Preliminary results suggest that a more accurate representation of soil moisture dynamics is achieved when calibrating against observed soil moisture and further improvement obtained with CRNS relative to PS. However, our results also suggest that a more accurate

  2. Investigation on the influence of BBO thermal-induced phase mismatching in 266nm UV laser conversion efficiency

    Yu, Kai; Jin, Guangyong; Yu, Miao; Huang, Zhulong; Zhai, Ruizhi; Wang, Lei

    2014-12-01

    266nm UV laser has a wide range of applications in many fields, such as laser medical treatment, laser processing, precision measure and other applications for the reason of its advantages in wavelength, small diffraction effects, high single-photon energy, and high resolution and so on. BBO crystals absorb parts of the fundamental laser energy and second harmonic laser energy are unavoidable, and thus the temperature raise, so that the existing crystal phase matching conditions change, resulting in phase mismatching in the high-power frequency doubling, greatly influence the 266nm UV laser conversion efficiency. To further study the effect of phase mismatching to conversion efficiency, and improve the conversion efficiency, output power and other output characteristics of 266nm laser, the article mainly describe from the following three aspects. Firstly, took the use of three-dimensional nonlinear crystal temperature distribution which is obtained, the process of BBO crystal thermal-induced phase mismatching is analyzed. Secondly, based on frequency doubling theory, the effects of the thermal-induced phase mismatching affected of conversion in crystals are analyzed. Combining with the phase mismatching of the three-dimensional distributions, the fourth harmonic conversion efficiency with thermal phase mismatching changes of BBO 266nm UV laser are simulated for the first time. Thirdly, by using MATLAB software, the effects of phase mismatching to conversion efficiency in crystal for different waist radius, 532nm laser power and the fundamental beam quality are simulated. The results indicate a good physical interpretation of reasons of high power laser frequency doubling system. It shows that the model established explains the reason of the reduction of conversion efficiency, output power and the beam quality excellently. All results make a leading sense to the research on the compensating of the phase mismatching and on the improvement of conversion efficiency.

  3. Mismatching Perspectives and Pacific Transculturality”

    Anne Holden Rønning

    2013-01-01

    Full Text Available Increased critical consciousness and awareness of interculturality in a global and glocal context at the beginning of the twenty - first century has increasingly used the concept of transculturation when discussing modernities. Politically transculturation can be used to describe processes of negotiation in contemporary society that lead to social awareness and solidarity, as well as ensuring the continuity of societies. The fusing of cultural forms leads to a mismatching of perspectives, hence some critics have preferred to use the terms translation and/or transliteracy to describe this concept. Transculturation is related to the “normal processes of artistic borrowing and influence, by which any cul ture makes part of its contribution to the conversation of mankind,” as Les Murray maintained, and “it engages multiple lines of difference simultaneous ly” with overlapping boundaries (Rogers 491. Referring to various authors and linking it to cultural ap propriation and border crossings, this article examines how the narrative expression of Both Sides of the Moon, to cite the title of Alan Duff’s book, is a key feature of Pacific writing, in an area where centuries of migration from near and far have expos ed different cultures to each other on social, political, linguistic and aesthetic levels. These ‘contact zones’, to use Mary Pratt’s words, provide the reader with constantly moving translated identities, cultural hybridity and a use of language that has a highly local significance in a global context.

  4. Novel Rx IQ mismatch compensation considering laser phase noise for CO-OFDM system

    Ma, Xiurong; Ding, Zhaocai; Li, Kun; Wang, Xiao

    2015-08-01

    In this paper, a novel compensation scheme for receiver (Rx) in-phase/quadrature (IQ) mismatch is proposed in coherent optical orthogonal frequency division multiplexing (CO-OFDM) system in the presence of laser phase noise. In this scheme, laser phase noise and channel distortion were combined as a new channel transfer factor, the IQ mismatch factor and initial channel transfer factor can be estimated independently based on the relationship of IQ mismatch factors. And the channel transfer factor can be updated on a symbol-by-symbol basis which retrieves an estimation of the phase noise value by extracting and averaging the phase drift of all OFDM sub-channels. Numerical results indicate that when the phase and amplitude mismatch are 10° and 2 dB respectively, a 1.6 dB optical signal-to noise ratio is improved at laser linewidth of 60 kHz. Furthermore, the complexity of the proposed method is analyzed in terms of the number of required complex multiplications per bit.

  5. Effects of GA mismatches on the structure and thermodynamics of RNA internal loops

    UV melting, CD and NMR studies indicate rGCGAGCG and rGCAGGCG from unusually stable duplexes of type a and b. The observed ΔG degree 37's in 1 M NaCl are -6.7 and -6.3 kcal/mol, respectively. For the related duplex, c,ΔG degree 37 is -4.2kcal/mol. The predicted ΔG degree 37 from nearest-neighbor parameters for all three duplexes is -4.7 kcal/mol. The results suggest a special interaction in the duplexes containing GA mismatches. Presumably, this is hydrogen bonding between G and A. While the thermodynamics for (rGCGAGCG)2 and (rGCAGGCG)2 are similar, CD and the imino region of the proton NMR spectra indicate their structures are different. The NMR spectrum of (rGCAGGCG)2 has a resonance corresponding to a hydrogen-bonded GA mismatch, while for (rGCGAGCG)2 no hydrogen-bonded imino proton is observed for the mismatch. The glycosidic torsion angles of the bases in the GA mismatches of (rGCAGGCG)2 and (rCGCAGGCG)2 are anti. Duplexes of type d, where X is A, G, or U, are more stable than e, and the stability differences are similar to those observed for f versus g. Thus, 3'-dangling ends in this system make contributions to duplex stability that are similar to contributions observed with fully paired duplexes

  6. Functional distance between recipient and donor HLA-DPB1 determines nonpermissive mismatches in unrelated HCT.

    Crivello, Pietro; Heinold, Andreas; Rebmann, Vera; Ottinger, Hellmut D; Horn, Peter A; Beelen, Dietrich W; Fleischhauer, Katharina

    2016-07-01

    The role of HLA amino acid (AA) polymorphism for the outcome of hematopoietic cell transplantation (HCT) is controversial, in particular for HLA class II. Here, we investigated this question in nonpermissive HLA-DPB1 T-cell epitope (TCE) mismatches reflected by numerical functional distance (FD) scores, assignable to all HLA-DPB1 alleles based on the combined impact of 12 polymorphic AAs. We calculated the difference in FD scores (ΔFD) of mismatched HLA-DPB1 alleles in patients and their 10/10 HLA-matched unrelated donors of 379 HCTs performed at our center for acute leukemia or myelodysplastic syndrome. Receiver-operator curve-based stratification into 2 ΔFD subgroups showed a significantly higher percentage of nonpermissive TCE mismatches for ΔFD >2.665, compared with ΔFD ≤2.665 (88% vs 25%, P 2.665 was significantly associated with overall survival (hazard ratio [HR], 1.40; 95% confidence interval [CI], 1.05-1.87; P TCE mismatches. There was a marked but not statistically significant increase in the hazards of relapse and nonrelapse mortality in the high ΔFD subgroup, whereas no differences were observed for acute and chronic graft-versus-host disease. Seven nonconservative AA substitutions in peptide-binding positions had a significantly stronger impact on ΔFD compared with 5 others (P = .0025), demonstrating qualitative differences in the relative impact of AA polymorphism in HLA-DPB1. The novel concept of ΔFD sheds new light onto nonpermissive HLA-DPB1 mismatches in unrelated HCT. PMID:27162243

  7. Test Mismatch in Switched-Current Circuits Using Wavelet Analysis"

    GUO Jierong; HE Yigang; LIU Meirong; TANG Shengxue; LI Hongmin

    2007-01-01

    Errors of mismatch and currents calibration caused by channel geometrical variety in switched-current are investigated in this paper. The relation and computing of mismatch and sensitivity are discussed also, and then a measure method of switched current mismatch using wavelet decomposition is proposed. A selected group of same transconductance is choosing as a cohort firstly, and the sensitivities of cohort in relation to the variation of transconductance are computed. Compared with the nominal deviation and tolerance borderline, the optimization and testing can be performed. As an example, a sixth order chebyshev low-pass filter is simulated and tested. The results have justified the reliability and feasibility of the method.

  8. Homogenizing the dose for adjacent fields

    Difficulties are found in radiotherapy in the determination of the gap which should be left among adjacent fields on the skin. In order to homogenize the dose at a given depth, measurements are done with a wood phantom using films, thermoluminescent dosemeters and ionization chambers. Field match is checked according to tables related in the literature and experimental data. Two tables of field separation are built at various depths, one for simultaneous adjacent fields and the other for non-simultaneous adjacent fields. Tables must be checked and the additional distances corrected for each field

  9. Mismatch oligonucleotides in human and yeast: guidelines for probe design on tiling microarrays

    Jee Justin

    2008-12-01

    Full Text Available Abstract Background Mismatched oligonucleotides are widely used on microarrays to differentiate specific from nonspecific hybridization. While many experiments rely on such oligos, the hybridization behavior of various degrees of mismatch (MM structure has not been extensively studied. Here, we present the results of two large-scale microarray experiments on S. cerevisiae and H. sapiens genomic DNA, to explore MM oligonucleotide behavior with real sample mixtures under tiling-array conditions. Results We examined all possible nucleotide substitutions at the central position of 36-nucleotide probes, and found that nonspecific binding by MM oligos depends upon the individual nucleotide substitutions they incorporate: C→A, C→G and T→A (yielding purine-purine mispairs are most disruptive, whereas A→X were least disruptive. We also quantify a marked GC skew effect: substitutions raising probe GC content exhibit higher intensity (and vice versa. This skew is small in highly-expressed regions (± 0.5% of total intensity range and large (± 2% or more elsewhere. Multiple mismatches per oligo are largely additive in effect: each MM added in a distributed fashion causes an additional 21% intensity drop relative to PM, three-fold more disruptive than adding adjacent mispairs (7% drop per MM. Conclusion We investigate several parameters for oligonucleotide design, including the effects of each central nucleotide substitution on array signal intensity and of multiple MM per oligo. To avoid GC skew, individual substitutions should not alter probe GC content. RNA sample mixture complexity may increase the amount of nonspecific hybridization, magnify GC skew and boost the intensity of MM oligos at all levels.

  10. L-band AlGaN/GaN Power Amplifier with Protection Against Load Mismatch

    Heijningen , M. van; Bent, G. van der; Houwen, E.H. van de; A Chowdhary; Vliet, F.E. van

    2013-01-01

    Solid-state power amplifiers need protection at the output to handle high reflections due to mismatch. Normally this is implemented by using a ferrite-based isolator. These are however large and bulky components. This paper presents a Gallium-Nitride power amplifier module with automatic protection against large reflections based on fold-back protection, by sensing the reflected power. Measurements have been performed on a 100 W L-band power amplifier module at full reflection (short at the o...

  11. Detect Adjacent Well by Analyzing Geomagnetic Anomalies

    Su Zhang

    2014-03-01

    Full Text Available This study describes a method of determining the position of adjacent well by analyzing geomagnetic anomalies in the drilling. In the experiment, put a casing in the geomagnetic field respectively to simulate 3 conditions, which are vertical well, deviated well and horizontal well. Study the interference of regional geomagnetic caused by casing, summary the law of the regional geomagnetic field anomalies caused by the adjacent casing. Experimental results show that: magnetic intensity distortion caused by deviated well is similar to that caused by horizontal well, but the distortion is different from vertical well. The scope and amplitude of N and E component magnetic intensity distortion will increase with the increase of casing inclination, meanwhile the scope and amplitude of V component distortion will decrease and the distortion value changes from negative to positive to the southwest of adjacent well. Through the analysis of geomagnetic anomalies, the position of the adjacent wells could be determined.

  12. 基于生态修复的青海湖沙柳河鱼道建设与维护%Construction and management of fish passage on Shaliu River adjacent to Qinghai Lake based on ecological restoration

    吴晓春; 史建全

    2014-01-01

    Gymnocypris przewalskii belongs to subfamily of Schizothoracinae, family of Cyprinidae and order of Cypriniformes, and it is an endemic species only distributed in the Qinghai Lake and its adjacent rivers and lakes. It possesses migration characteristics, and goes upstream from the lake to surrounding rivers in the reproduction season. However, several dams built on the rivers flowing into the lake with the agricultural development in the Qinghai Lake region since 1970s, severely blocked its migration passages, leading to obviously decreasing of resources of Gymnocypris przewalskii in recent years. To protect Gymnocypris przewalskii, three kinds of fish passages on the dams of rivers flowing into the Qinghai Lake were introduced, which were types of guide wall, simple steel modular and ladder slope, respectively. 1) The guide wall type fish passage was separately constructed on the dams in the Shaliu River, Quanji River and Haergai River in 2006, which were built along river side, but it had several defects because of the lack of experience. 2) The simple steel modular type fish passage was designed and constructed on the dam of the Shaliu River in 2008, Based on biological characteristics of Gymnocypris przewalskii and the basic situation of the dam and environmental conditions of the Shaliu River. After considering inlet location of fish passage, suction flow and hydraulic conditions within passage, the inlet was located at the area with aggregated fish and slow current velocity. The water flow was 0.5 m/s, the current velocity was 0.1-0.25 m3/s, as well as the slope of 1:10 within fish passage. 3) The ladder slope type fish passage was built on the dam of Shaliu River in 2010, which used two types open fishponds of multi-ladder and slight slope. Fish passage without hindrance had been almost come to be real through this type. This type was designed according to basic theories and related parameters of simple fish passage. The length was 80 m and the width was 1

  13. Adjacency Algebra of Unitary Cayley Graph

    A. Satyanarayana Reddy

    2013-02-01

    Full Text Available A few properties of unitary Cayley graphs are explored using their eigenvalues. It is shown that the adjacency algebra of a unitary Cayley graph is a coherent algebra. Finally, a class of unitary Cayley graphs that are distance regular are also obtained.Key Words: Adjacency Algebra, Circulant Graph, Coherent Algebra, Distance Regular Graph,Ramanujan's sum .AMS(2010: 05C25, 05C50

  14. Protein-protein interactions in DNA mismatch repair.

    Friedhoff, Peter; Li, Pingping; Gotthardt, Julia

    2016-02-01

    The principal DNA mismatch repair proteins MutS and MutL are versatile enzymes that couple DNA mismatch or damage recognition to other cellular processes. Besides interaction with their DNA substrates this involves transient interactions with other proteins which is triggered by the DNA mismatch or damage and controlled by conformational changes. Both MutS and MutL proteins have ATPase activity, which adds another level to control their activity and interactions with DNA substrates and other proteins. Here we focus on the protein-protein interactions, protein interaction sites and the different levels of structural knowledge about the protein complexes formed with MutS and MutL during the mismatch repair reaction. PMID:26725162

  15. Understanding the Consequences of Property Rights Mismatches: a Case Study of New Zealand's Marine Resources

    Tracy Yandle

    2007-12-01

    Full Text Available Within fisheries and natural resource management literature, there is considerable discussion about the key roles that property rights can play in building biologically and socially sustainable resource management regimes. A key point of agreement is that secure long-term property rights provide an incentive for resource users to manage the resource sustainably. However, property rights mismatches create ambiguity and conflict in resource use. Though the term mismatches is usually associated with problems in matching temporal and spatial resource characteristics with institutional characteristics, I expand it here to include problems that can arise when property rights are incompletely defined or incompletely distributed. Property rights mismatches are particularly likely to occur over marine resources, for which multiple types of resource and resource user can be engaged and managed under a variety of regulatory regimes. I used New Zealand's marine resources to examine the causes and consequences of these property rights mismatches. New Zealand is particularly interesting because its property-rights-based commercial fishing regime, in the form of individual transferable quotas, has attracted considerable positive attention. However, my review of the marine natural resource management regime from a broader property rights perspective highlights a series of problems caused by property rights mismatches, including competition for resources among commercial, customary, and recreational fishers; spatial conflict among many marine resource users; and conflicting incentives and objectives for the management of resources over time. The use of a property rights perspective also highlights some potential solutions such as the layering of institutional arrangements and the improvement of how property rights are defined to encourage long-term sustainability.

  16. Mismatch characteristics of optical parametric chirped pulse amplification

    Novák, Ondřej; Turčičová, Hana; Divoký, Martin; Huynh, Jaroslav; Straka, Petr

    2014-01-01

    Roč. 11, č. 2 (2014), 1-7. ISSN 1612-2011 R&D Projects: GA ČR GA202/06/0814; GA MŠk(CZ) LC528 Institutional support: RVO:68378271 Keywords : phase matching * phase mismatch * beam mismatch * broadband amplification * parametric amplifiers * OPCPA * iodine laser Subject RIV: BH - Optics , Masers, Laser s Impact factor: 2.458, year: 2014

  17. A Trainable Neuromorphic Integrated Circuit that Exploits Device Mismatch

    Thakur, Chetan Singh; Wang, Runchun; Hamilton, Tara Julia; Tapson, Jonathan; van Schaik, Andre

    2015-01-01

    Random device mismatch that arises as a result of scaling of the CMOS (complementary metal-oxide semi-conductor) technology into the deep submicron regime degrades the accuracy of analogue circuits. Methods to combat this increase the complexity of design. We have developed a novel neuromorphic system called a Trainable Analogue Block (TAB), which exploits device mismatch as a means for random projections of the input to a higher dimensional space. The TAB framework is inspired by the princip...

  18. Rhabdomyosarcoma in patients with constitutional mismatch-repair-deficiency syndrome

    Kratz, Christian P; Holter, Spring; Etzler, Julia; Lauten, Melchior; Pollett, Aaron; Charlotte M. Niemeyer; Gallinger, Steven; Wimmer, Katharina

    2009-01-01

    Abstract Background: Biallelic germline mutations in the mismatch repair genes MLH1, MSH2, MSH6 or PMS2 cause a recessive childhood cancer syndrome characterized by early-onset malignancies and signs reminiscent of neurofibromatosis type 1 (NF1). Alluding to the underlying genetic defect we refer to this syndrome as constitutional mismatch repair-deficiency (CMMR-D) syndrome. The tumor spectrum of CMMR-D syndrome includes hematological neoplasias, brain tumors and Lynch syndrome as...

  19. The effects of nucleotides on MutS-DNA binding kinetics clarify the role of MutS ATPase activity in mismatch repair

    Jacobs-Palmer, Emily; Hingorani, Manju M.

    2006-01-01

    MutS protein initiates mismatch repair with recognition of a non-Watson-Crick base pair or base insertion/deletion site in DNA, and its interactions with DNA are modulated by ATPase activity. Here we present a kinetic analysis of these interactions, including the effects of ATP binding and hydrolysis, reported directly from the mismatch site by 2-aminopurine fluorescence. When free of nucleotides, the T. aquaticus MutS dimer binds a mismatch rapidly (kON = 3 x 106 M−1s−1) and forms a stable c...

  20. Image edge-enhancement in optical microscopy with a phase mismatched spiral phase plate

    Shibiao Wei; Jing Bu; Siwei Zhu; Xiaocong Yuan

    2011-01-01

    @@ We present a spiral phase filtering system with a large tolerance for edge enhancement of both phase and amplitude objects in optical microscopy.The method is based on a Fourier 4-f spatial filtering system.A phase mismatched spiral phase plate (SPP) fabricated by electron beam lithography is employed as the radial Hilbert transform for image edge enhancement.Compared with holography, SPP is simple,economical, reliable, and easy to integrate.%We present a spiral phase filtering system with a large tolerance for edge enhancement of both phase and amplitude objects in optical microscopy. The method is based on a Fourier 4-f spatial filtering system.A phase mismatched spiral phase plate (SPP) fabricated by electron beam lithography is employed as the radial Hilbert transform for image edge enhancement. Compared with holography, SPP is simple,economical, reliable, and easy to integrate.

  1. Molecularly resolved label-free sensing of single nucleobase mismatches by interfacial LNA probes.

    Mishra, Sourav; Lahiri, Hiya; Banerjee, Siddhartha; Mukhopadhyay, Rupa

    2016-05-01

    So far, there has been no report on molecularly resolved discrimination of single nucleobase mismatches using surface-confined single stranded locked nucleic acid (ssLNA) probes. Herein, it is exemplified using a label-independent force-sensing approach that an optimal coverage of 12-mer ssLNA sensor probes formed onto gold(111) surface allows recognition of ssDNA targets with twice stronger force sensitivity than 12-mer ssDNA sensor probes. The force distributions are reproducible and the molecule-by-molecule force measurements are largely in agreement with ensemble on-surface melting temperature data. Importantly, the molecularly resolved detection is responsive to the presence of single nucleobase mismatches in target sequences. Since the labelling steps can be eliminated from protocol, and each force-based detection event occurs within milliseconds' time scale, the force-sensing assay is potentially capable of rapid detection. The LNA probe performance is indicative of versatility in terms of substrate choice - be it gold (for basic research and array-based applications) or silicon (for 'lab-on-a-chip' type devices). The nucleic acid microarray technologies could therefore be generally benefited by adopting the LNA films, in place of DNA. Since LNA is nuclease-resistant, unlike DNA, and the LNA-based assay is sensitive to single nucleobase mismatches, the possibilities for label-free in vitro rapid diagnostics based on the LNA probes may be explored. PMID:27025649

  2. An unexpected sequence of events: mismatch detection in the human hippocampus

    Kumaran, D.; Maguire, E. A.

    2006-01-01

    The ability to identify and react to novelty within the environment is fundamental to survival. Computational models emphasize the potential role of the hippocampus in novelty detection, its unique anatomical circuitry making it ideally suited to act as a comparator between past and present experience. The hippocampus, therefore, is viewed to detect associative mismatches between what is expected based on retrieval of past experience and current sensory input. However, direct evidence that th...

  3. An Unexpected Sequence of Events: Mismatch Detection in the Human Hippocampus

    Kumaran, Dharshan; Maguire, Eleanor A.

    2006-01-01

    The ability to identify and react to novelty within the environment is fundamental to survival. Computational models emphasize the potential role of the hippocampus in novelty detection, its unique anatomical circuitry making it ideally suited to act as a comparator between past and present experience. The hippocampus, therefore, is viewed to detect associative mismatches between what is expected based on retrieval of past experience and current sensory input. However, direct evidence that th...

  4. Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity.

    Anderson, Emily M; Haupt, Amanda; Schiel, John A; Chou, Eldon; Machado, Hidevaldo B; Strezoska, Žaklina; Lenger, Steve; McClelland, Shawn; Birmingham, Amanda; Vermeulen, Annaleen; Smith, Anja van Brabant

    2015-10-10

    The discovery that the bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) acquired immune system can be utilized to create double-strand breaks (DSBs) in eukaryotic genomes has resulted in the ability to create genomic changes more easily than with other genome engineering techniques. While there is significant potential for the CRISPR-Cas9 system to advance basic and applied research, several unknowns remain, including the specificity of the RNA-directed DNA cleavage by the small targeting RNA, the CRISPR RNA (crRNA). Here we describe a novel synthetic RNA approach that allows for high-throughput gene editing experiments. This was used with a functional assay for protein disruption to perform high-throughput analysis of crRNA activity and specificity. We performed a comprehensive test of target cleavage using crRNAs that contain one and two nucleotide mismatches to the DNA target in the 20mer targeting region of the crRNA, allowing for the evaluation of hundreds of potential mismatched target sites without the requirement for the off-target sequences and their adjacent PAMs to be present in the genome. Our results demonstrate that while many crRNAs are functional, less than 5% of crRNAs with two mismatches to their target are effective in gene editing; this suggests an overall high level of functionality but low level of off-targeting. PMID:26189696

  5. Mis-Match Limit Load Analyses and Fracture Mechanics Assessment for Welded Pipe with Circumferential Crack at the Center of Weldment

    In this paper, limit load analyses and fracture mechanics analyses were conducted via finite element analyses for the welded pipe with circumferential crack at the center of the weldment. Systematic changes for strength mismatch ratio, width of weldment, crack shape and thickness ratio of the pipe were considered to provide strength mismatch limit load. And J-integral calculations based on reference stress method were conducted for two materials, stainless steel and ferritic steel. Reference stress defined by provided strength mis-match limit load gives much more accurate J-integral

  6. Functional interactions and signaling properties of mammalian DNA mismatch repair proteins.

    Bellacosa, A

    2001-11-01

    The mismatch repair (MMR) system promotes genomic fidelity by repairing base-base mismatches, insertion-deletion loops and heterologies generated during DNA replication and recombination. This function is critically dependent on the assembling of multimeric complexes involved in mismatch recognition and signal transduction to downstream repair events. In addition, MMR proteins coordinate a complex network of physical and functional interactions that mediate other DNA transactions, such as transcription-coupled repair, base excision repair and recombination. MMR proteins are also involved in activation of cell cycle checkpoint and induction of apoptosis when DNA damage overwhelms a critical threshold. For this reason, they play a role in cell death by alkylating agents and other chemotherapeutic drugs, including cisplatin. Inactivation of MMR genes in hereditary and sporadic cancer is associated with a mutator phenotype and inhibition of apoptosis. In the future, a deeper understanding of the molecular mechanisms and functional interactions of MMR proteins will lead to the development of more effective cancer prevention and treatment strategies. PMID:11687886

  7. Parametric Adaptive Radar Detector with Enhanced Mismatched Signals Rejection Capabilities

    Liu Bin

    2010-01-01

    Full Text Available We consider the problem of adaptive signal detection in the presence of Gaussian noise with unknown covariance matrix. We propose a parametric radar detector by introducing a design parameter to trade off the target sensitivity with sidelobes energy rejection. The resulting detector merges the statistics of Kelly's GLRT and of the Rao test and so covers Kelly's GLRT and the Rao test as special cases. Both invariance properties and constant false alarm rate (CFAR behavior for this detector are studied. At the analysis stage, the performance of the new receiver is assessed and compared with several traditional adaptive detectors. The results highlight better rejection capabilities of this proposed detector for mismatched signals. Further, we develop two two-stage detectors, one of which consists of an adaptive matched filter (AMF followed by the aforementioned detector, and the other is obtained by cascading a GLRT-based Subspace Detector (SD and the proposed adaptive detector. We show that the former two-stage detector outperforms traditional two-stage detectors in terms of selectivity, and the latter yields more robustness.

  8. Adjacent Instability after Instrumented Lumbar Fusion.

    Wen-Jer Chen

    2003-11-01

    Full Text Available The invention of pedicle screw instrumentation has greatly improved outcomes ofspinal fusion, which has become the treatment of choice for lumbar spondylolisthesis. Asresearchers accumulate experience, both theoretical and clinical advances are continuallybeing reported. A review of the literature and the experience of the authors show that thedevelopment of adjacent instability, as in the breakdown of a neighboring unfixed motionsegment, is a common consequence of an instrumented lumbar spine. This article reviewsthe risk factors and surgical treatment of adjacent instability. The authors believe that properpreoperative planning and complete surgical procedures are imperative to prevent adjacentinstability. For those who need revision surgery, meticulous surgical techniques can achievesatisfactory results.

  9. On the complexity of adjacent resource scheduling

    Duin, C.W.; Sluis, van, P.

    2004-01-01

    We study the problem of scheduling resource(s) for jobs in an adjacent manner (ARS). The problem relates to fixed interval scheduling on the one hand, and to the problem of two-dimensional strip packing on the other hand. Further, there is a close relation with multiprocessor scheduling. A distinguishing characteristic is the constraint of resource-adjacency. As an application of ARS, consider an airport where passengers check in for their flight, joining lines before one or more desks; at th...

  10. KAMG: A Tool for Converting Blood Ties and Affinity Ties into Adjacency Matrices

    Hang Xiong

    2016-07-01

    Full Text Available Kinship Adjacency Matrix Generator (KAMG is a browser-based software for creating adjacency matrices using the information of kinship ties. Specifically, it is capable of converting the family trees in the format of GEDCOM files into adjacency matrices of blood relationship. With the data of intermarriages between different families, it can further create the adjacency matrix of affinity relationship for the families. The outcomes can be directly used to create networks. KAMG is written in JavaScript and implemented on web browsers. It is completely open source and the source code is publicly available on GitHub.

  11. Direct Mismatch Characterization of femto-Farad Capacitors

    Omran, Hesham

    2015-08-17

    Reducing the capacitance of programmable capacitor arrays, commonly used in analog integrated circuits, is necessary for low-energy applications. However, limited mismatch data is available for small capacitors. We report mismatch measurement for a 2fF poly-insulator-poly (PIP) capacitor, which is the smallest reported PIP capacitor to the best of the authors’ knowledge. Instead of using complicated custom onchip circuitry, direct mismatch measurement is demonstrated and verified using Monte Carlo Simulations and experimental measurements. Capacitive test structures composed of 9 bit programmable capacitor arrays (PCAs) are implemented in a low-cost 0:35m CMOS process. Measured data is compared to mismatch of large PIP capacitors, theoretical models, and recently published data. Measurement results indicate an estimated average relative standard deviation of 0.43% for the 2fF unit capacitor, which is better than the reported mismatch of metal-oxide-metal (MOM) fringing capacitors implemented in an advanced 32nm CMOS process.

  12. Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR

    Seiki Kuramitsu

    2013-03-01

    Full Text Available Polymerase chain reaction (PCR-related technologies are hampered mainly by two types of error: nonspecific amplification and DNA polymerase-generated mutations. Here, we report that both errors can be suppressed by the addition of a DNA mismatch-recognizing protein, MutS, from a thermophilic bacterium. Although it had been expected that MutS has a potential to suppress polymerase-generated mutations, we unexpectedly found that it also reduced nonspecific amplification. On the basis of this finding, we propose that MutS binds a mismatched primer-template complex, thereby preventing the approach of DNA polymerase to the 3' end of the primer. Our simple methodology improves the efficiency and accuracy of DNA amplification and should therefore benefit various PCR-based applications, ranging from basic biological research to applied medical science.

  13. Mismatch oxidation assay: detection of DNA mutations using a standard UV/Vis microplate reader.

    Tabone, Tania; Sallmann, Georgina; Cotton, Richard G H

    2009-01-01

    Simple, low-cost mutation detection assays that are suitable for low-throughput analysis are essential for diagnostic applications where the causative mutation may be different in every family. The mismatch oxidation assay is a simple optical absorbance assay to detect nucleotide substitutions, insertions, and deletions in heteroduplex DNA. The method relies on detecting the oxidative modification products of mismatched thymine and cytosine bases by potassium permanganate as it is reduced to manganese dioxide. This approach, unlike other methods commonly used to detect sequence variants, does not require costly labeled probes or primers, toxic chemicals, or a time-consuming electrophoretic separation step. The oxidation rate, and hence the presence of a sequence variant, is detected by measuring the formation of the potassium permanganate reduction product (hypomanganate diester), which absorbs at the 420-nm visible wavelength, using a standard UV/vis microplate reader. PMID:19768598

  14. An analytical model for finite radius dual-beam mode-mismatched thermal lens spectroscopy

    Sabaeian, Mohammad; Nadgaran, Hamid

    2013-10-01

    In this work, a new model for dual-beam mode-mismatch thermal lens spectroscopy is presented. The model was based on a new analytical solution of time-dependent heat equation for finite radius cylindrical samples exposed to TEM00 excitation laser beams. The Fresnel diffraction integration method was used to calculate time-dependent on-axis probe beam intensity. All aberrations in thermal lens were taken into account. The model yields accurate values for absorption coefficient and thermal diffusivity of methylene blue aqueous solution. Furthermore, the optimized mode-mismatched version of this model when applied to pure water as a very low absorbent yields its absorption coefficient and thermal diffusivity values close to literature data. In contrast to traditional model, this model does not need to omit any term in its theory to fit the experimental data.

  15. Adjacent stimulation and measurement patterns considered harmful

    We characterize the ability of electrical impedance tomography (EIT) to distinguish changes in internal conductivity distributions, and analyze it as a function of stimulation and measurement patterns. A distinguishability measure, z, is proposed which is related to the signal-to-noise ratio of a medium and to the probability of detection of conductivity changes in a region of interest. z is a function of the number of electrodes, the EIT stimulation and measurement protocol, the stimulation amplitude, the measurement noise, and the size and location of the contrasts. Using this measure we analyze various choices of stimulation and measurement patterns under the constraint of medical electrical safety limits (maximum current into the body). Analysis is performed for a planar placement of 16 electrodes for simulated 3D tank and chest shapes, and measurements in a saline tank. Results show that the traditional (and still most common) adjacent stimulation and measurement patterns have by far the poorest performance (by 6.9 ×). Good results are obtained for trigonometric patterns and for pair drive and measurement patterns separated by over 90°. Since the possible improvement over adjacent patterns is so large, we present this result as a call to action: adjacent patterns are harmful, and should be abandoned. We recommend using pair drive and measurement patterns separated by one electrode less than 180°. We describe an approach to modify an adjacent pattern EIT system by adjusting electrode placement

  16. Refractive index and dielectric constants of lattice matched and mismatched Cdx Zn1-x Sey Te1-y quaternary alloys

    We report on a study of optical properties, namely the refractive index and high-frequency and static dielectric constants of zinc-blende Cdx Zn1-x Sey Te1-y under conditions of lattice matching and lattice mismatching to ZnTe substrates. The calculations are mainly based on the pseudopotential approach under a virtual crystal approximation. Our results show that the Cdx Zn1-x Sey Te1-y lattice matched to ZnTe is a direct band-gap semiconductor for all possible values of x and y (0 ≤x≤1, 0≤y≤0.879). The studied features are found to be strongly dependent on the lattice mismatch percentage. The present investigation provides more opportunities to obtain diverse refractive indices and dielectric constants, while still controlling the composition parameters (x and y) and/or the lattice mismatch percentage.

  17. Mismatch repair and treatment resistance in ovarian cancer

    The treatment of ovarian cancer is hindered by intrinsic or acquired resistance to platinum-based chemotherapy. The aim of this study is to determine the frequency of mismatch repair (MMR) inactivation in ovarian cancer and its association with resistance to platinum-based chemotherapy. We determined, microsatellite instability (MSI) as a marker for MMR inactivation (analysis of BAT25 and BAT26), MLH1 promoter methylation status (methylation specific PCR on bisulfite treated DNA) and mRNA expression of MLH1, MSH2, MSH3, MSH6 and PMS2 (quantitative RT-PCR) in 75 ovarian carcinomas and eight ovarian cancer cell lines MSI was detected in three of the eight cell lines i.e. A2780 (no MLH1 mRNA expression due to promoter methylation), SKOV3 (no MLH1 mRNA expression) and 2774 (no altered expression of MMR genes). Overall, there was no association between cisplatin response and MMR status in these eight cell lines. Seven of the 75 ovarian carcinomas showed MLH1 promoter methylation, however, none of these showed MSI. Forty-six of these patients received platinum-based chemotherapy (11 non-responders, 34 responders, one unknown response). The resistance seen in the eleven non-responders was not related to MSI and therefore also not to MMR inactivation. No MMR inactivation was detected in 75 ovarian carcinoma specimens and no association was seen between MMR inactivation and resistance in the ovarian cancer cell lines as well as the ovarian carcinomas. In the discussion, the results were compared to that of twenty similar studies in the literature including in total 1315 ovarian cancer patients. Although no association between response and MMR status was seen in the primary tumor the possible role of MMR inactivation in acquired resistance deserves further investigation

  18. Soil Structure Interaction between Two Adjacent Buildings under Earthquake Load

    Mahmoud Yahyai; Masoud Mirtaheri; Mehrab Mahoutian; Amir S. Daryan

    2008-01-01

    In some cases, tall buildings are located in geotechnically unsuitable places, due to their high ratio of height to width; there is risk of uplift and other effects such as overturning and reduction structure serviceability during earthquake. This research is aimed to evaluate the effect of Soil-Structure Interaction (SSI) on seismic behavior of two adjacent 32 story buildings such as time period, base shear and displacements. The interaction effects are investigated for variable distance bet...

  19. Disrupted Turn Adjacency and Coherence Maintenance in Instant Messaging Conversations

    Berglund, Therese Örnberg

    2009-01-01

    This article reports on a study of coherence in text-based log files from 120 two-party Instant Messaging (IM) conversations among a group of international students at a design school. The goals of the study were to investigate whether disrupted turn adjacency was an obstacle to coherence and to identify the linguistic strategies employed to maintain coherence. Additional signs of problematic coherence creation were also investigated, focusing on explicit signs of miscommunication. In this pa...

  20. Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains

    Milovanovic, Dragomir; Honigmann, Alf; Koike, Seiichi; Göttfert, Fabian; Pähler, Gesa; Junius, Meike; Müllar, Stefan; Diederichsen, Ulf; Janshoff, Andreas; Grubmüller, Helmut; Risselada, Herre J.; Eggeling, Christian; Hell, Stefan W.; van den Bogaart, Geert; Jahn, Reinhard

    2015-01-01

    The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic and ionic interactions with membrane lipids as well as of specific protein-protein interactions. Here using plasma membrane-resident SNARE proteins as model, we show that hydrophobic mismatch between the length of transmembrane domains (TMDs) and the thickness of the lipid membrane suffices to induce clustering of proteins. Even when the TMDs differ in length by only a single residue, hydrophobic mismatch can segregate structurally closely homologous membrane proteins in distinct membrane domains. Domain formation is further fine-tuned by interactions with polyanionic phosphoinositides and homo and heterotypic protein interactions. Our findings demonstrate that hydrophobic mismatch contributes to the structural organization of membranes.

  1. Bilayer thickness mismatch controls domain size in biomimetic membranes

    Heberle, Frederick A.; Petruzielo, Robin S.; Pan, Jianjun; Drazba, Paul; Kučerka, Norbert; Standaert, Robert F.; Feigenson, Gerald W.; Katsara, John

    2013-03-01

    In order to promote functionality, cells may alter the spatial organization of membrane lipids and proteins, including separation of liquid phases into distinct domains. In model membranes, domain size and morphology depend strongly on composition and temperature, but the physicochemical mechanisms controlling them are poorly understood. Theoretical work suggests a role for interfacial energy at domain boundaries, which may be driven in part by thickness mismatch between a domain and its surrounding bilayer. However, no direct evidence linking thickness mismatch to domain size in free-standing bilayers has been reported. We describe the use of Small Angle Neutron Scattering (SANS) to detect domains in simplified lipid-only models that mimic the composition of plasma membrane. We find that domain size is controlled by the degree of acyl chain unsaturation of low-melting temperature lipids, and that this size transition is correlated to changes in the thickness mismatch between coexisting liquid phases.

  2. Interobserver variability in the evaluation of mismatch repair protein immunostaining

    Klarskov, Louise; Ladelund, Steen; Holck, Susanne; Roenlund, Karina; Lindebjerg, Jan; Elebro, Jacob; Halvarsson, Britta; von Salomé, Jenny; Bernstein, Inge; Nilbert, Mef

    2010-01-01

    reliability are lacking. We therefore evaluated interobserver variability among 6 pathologists, 3 experienced gastrointestinal pathologists and 3 residents. In total, 225 immunohistochemically stained colorectal cancers were evaluated as having normal, weak, loss of, or nonevaluable mismatch repair protein......Immunohistochemical staining for mismatch repair proteins has during recent years been established as a routine analysis in many pathology laboratories with the aim to identify tumors linked to the hereditary nonpolyposis colorectal cancer syndrome. Despite widespread application, data on...... variability was considerable, though experienced pathologists and residents reached the same level of consensus. Because results from immunohistochemical mismatch repair protein stainings are used for decisions on mutation analysis and as an aid in the interpretation of gene variants of unknown significance...

  3. Interobserver variability in the evaluation of mismatch repair protein immunostaining

    Klarskov, Louise Laurberg; Ladelund, Steen; Holck, Susanne;

    2010-01-01

    reliability are lacking. We therefore evaluated interobserver variability among 6 pathologists, 3 experienced gastrointestinal pathologists and 3 residents. In total, 225 immunohistochemically stained colorectal cancers were evaluated as having normal, weak, loss of, or nonevaluable mismatch repair protein...... variability was considerable, though experienced pathologists and residents reached the same level of consensus. Because results from immunohistochemical mismatch repair protein stainings are used for decisions on mutation analysis and as an aid in the interpretation of gene variants of unknown significance...... in hereditary nonpolyposis colorectal cancer, the interobserver variability identified highlights the need for quality assessment programs, including guidelines for classification of different expression patterns....

  4. STUDY OF MISMATCHED FILTERING OF PASSIVE RADAR USING TV SIGNAL

    Sun Xiaowen; Zhang Linrang; Wu Shunjun

    2006-01-01

    This letter demonstrates the structure of the passive radar using TV signals. Because the TV signal is a kind of pseudoperiodic signal, the matched filtering of color TV signals would yield high sidelobes which cause the range ambiguity. To overcome this problem, the mismatched filter is proposed to suppress the correlation sidelobes of matched filtering of TV signals. By utilizing the iteration process, this method could achieve the required peak sidelobe level. The impacts of the noise and target movement on mismatched filtering are also analysed. Simulation results are included to demonstrate the effectiveness of the proposed technique.

  5. Advanced radar detection schemes under mismatched signal models

    Bandiera, Francesco

    2009-01-01

    Adaptive detection of signals embedded in correlated Gaussian noise has been an active field of research in the last decades. This topic is important in many areas of signal processing such as, just to give some examples, radar, sonar, communications, and hyperspectral imaging. Most of the existing adaptive algorithms have been designed following the lead of the derivation of Kelly's detector which assumes perfect knowledge of the target steering vector. However, in realistic scenarios, mismatches are likely to occur due to both environmental and instrumental factors. When a mismatched signal

  6. Visual mismatch negativity reveals automatic detection of sequential regularity violation

    Gábor Stefanics

    2011-05-01

    Full Text Available Sequential regularities are abstract rules based on repeating sequences of environmental events, which are useful to make predictions about future events. As the processes underlying visual mismatch negativity (vMMN are sensitive to complex stimulus changes, this event-related potential component, like its auditory counterpart, may be an index of a primitive system of intelligence. Here we tested whether the visual system is capable to detect abstract sequential regularity in unattended stimulus sequences. In our first experiment we investigated the emergence of vMMN and other change-related activity to stimuli violating abstract rules. Red and green disk patterns were delivered in pairs. When in the majority of pairs the colors were identical within the pairs, deviant pairs with different colors for the second member of the pair elicited vMMN. Spatially more extended vMMN responses with longer latency were observed for deviants with 10% compared to 30% probability. In our second experiment utilizing oddball sequences, we tested the emergence of vMMN to violations of a concrete, feature-based rule of a repetition of a standard color. Deviant colors elicited a vMMN response in the oddball sequences. VMMN was larger for the second member of the pair, i.e. after a shorter stimulus onset asynchrony (SOA. This result corresponds to the expected SOA/(vMMN relationship. Our results show that the system underlying vMMN is sensitive to abstract probability rules and this component can be considered as a correlate of violated predictions about the characteristics of environmental events.

  7. A Provably Efficient Algorithm for the k-Mismatch Average Common Substring Problem.

    Thankachan, Sharma V; Apostolico, Alberto; Aluru, Srinivas

    2016-06-01

    Alignment-free sequence comparison methods are attracting persistent interest, driven by data-intensive applications in genome-wide molecular taxonomy and phylogenetic reconstruction. Among all the methods based on substring composition, the average common substring (ACS) measure admits a straightforward linear time sequence comparison algorithm, while yielding impressive results in multiple applications. An important direction of this research is to extend the approach to permit a bounded edit/hamming distance between substrings, so as to reflect more accurately the evolutionary process. To date, however, algorithms designed to incorporate k ≥ 1 mismatches have O(n(2)) worst-case time complexity, where n is the total length of the input sequences. On the other hand, accounting for mismatches has shown to lead to much improved classification, while heuristics can improve practical performance. In this article, we close the gap by presenting the first provably efficient algorithm for the k-mismatch average common string (ACSk) problem that takes O(n) space and O(n log(k) n) time in the worst case for any constant k. Our method extends the generalized suffix tree model to incorporate a carefully selected bounded set of perturbed suffixes, and can be applied to other complex approximate sequence matching problems. PMID:27058840

  8. Cross-scale feedbacks and scale mismatches as influences on cultural services and the resilience of protected areas.

    Maciejewski, Kristine; De Vos, Alta; Cumming, Graeme S; Moore, Christine; Biggs, Duan

    2015-01-01

    Protected areas are a central strategy for achieving global conservation goals, but their continued existence depends heavily on maintaining sufficient social and political support to outweigh economic interests or other motives for land conversion. Thus, the resilience of protected areas can be considered a function of their perceived benefits to society. Nature-based tourism (NBT), a cultural ecosystem service, provides a key source of income to protected areas, facilitating a sustainable solution to conservation. The ability of tourism to generate income depends, however, on both the scales at which this cultural service is provided and the scales at which tourists respond to services on offer. This observation raises a set of location-, context-, and scale-related questions that need to be confronted before we can understand and value cultural service provision appropriately. We combine elements of resilience analysis with a systems ecology framework and apply this to NBT in protected areas to investigate cross-scale interactions and scale mismatches. We postulate that cross-scale effects can either have a positive effect on protected area resilience or lead to scale mismatches, depending on their interactions with cross-scale feedbacks. To demonstrate this, we compare spatial scales and nested levels of institutions to develop a typology of scale mismatches for common scenarios in NBT. In our new typology, the severity of a scale mismatch is expressed as the ratio of spatial scale to institutional level, producing 25 possible outcomes with differing consequences for system resilience. We predict that greater differences between interacting scales and levels, and greater magnitudes of cross-scale interactions, will lead to greater magnitudes of scale mismatch. Achieving a better understanding of feedbacks and mismatches, and finding ways of aligning spatial and institutional scales, will be critical for strengthening the resilience of protected areas that

  9. Adjacent Segment Pathology after Lumbar Spinal Fusion.

    Lee, Jae Chul; Choi, Sung-Woo

    2015-10-01

    One of the major clinical issues encountered after lumbar spinal fusion is the development of adjacent segment pathology (ASP) caused by increased mechanical stress at adjacent segments, and resulting in various radiographic changes and clinical symptoms. This condition may require surgical intervention. The incidence of ASP varies with both the definition and methodology adopted in individual studies; various risk factors for this condition have been identified, although a significant controversy still exists regarding their significance. Motion-preserving devices have been developed, and some studies have shown their efficacy of preventing ASP. Surgeons should be aware of the risk factors of ASP when planning a surgery, and accordingly counsel their patients preoperatively. PMID:26435804

  10. Educational Mismatch and Spatial Flexibility in Italian Local Labour Markets

    Croce, Giuseppe; Ghignoni, Emanuela

    2015-01-01

    According to recent literature, this paper highlights the relevance of spatial mobility as an explanatory factor of the individual risk of job-education mismatch. To investigate this causal link, we use individual information about daily home-to-work commuting time and choices to relocate in a different local area to get a job. Our model takes…

  11. The dual nature of mismatch repair as antimutator and mutator

    Bak, Sara Thornby; Sakellariou, Despoina; Pena Diaz, Javier

    2014-01-01

    DNA is constantly under attack by a number of both exogenous and endogenous agents that challenge its integrity. Among the mechanisms that have evolved to counteract this deleterious action, mismatch repair (MMR) has specialized in removing DNA biosynthetic errors that occur when replicating the...

  12. Highly tunable mismatched twin-core fibre filters

    Ortega, B.; Dong, L.

    1998-01-01

    We demonstrated a reproducible and accurate way for adjusting wavelength of mismatched twin-core filters over few hundred nanometres from the same fibre design. The wavelength control together with strength control by fibre length adjustment allows complex filter profiles to be implemented by cascading. These filters are highly stable to changes in temperature and strain.

  13. Investigating Interaural Frequency-Place Mismatches via Bimodal Vowel Integration

    Guérit, François; Santurette, Sébastien; Chalupper, Josef;

    2014-01-01

    For patients having residual hearing in one ear and a cochlear implant (CI) in the opposite ear, interaural place-pitch mismatches might be partly responsible for the large variability in individual benefit. Behavioral pitch-matching between the two ears has been suggested as a way to individuali...... difficulties in bimodal fusion of the two formants....

  14. Mismatch-shaping switching for two-capacitor DAC

    Steensgaard-Madsen, Jesper; Moon, U.; Temes, G.C.

    1998-01-01

    A mismatch-shaping scheme is proposed for a two-capacitor digital-to-analogue converter (DAC). It uses a delta-sigma loop for finding the optimal switching sequence for each input word. Simulations indicate that the scheme can be used for the realisation of DACs with 16 bit linearity and SNR perf...

  15. Feature Mismatch: Deponency in Indo-European Languages

    Grestenberger, Laura

    2014-01-01

    This thesis investigates "voice mismatch verbs" (deponents), verbs that take non-active morphology but are used in syntactically active environments. The focus is on the non-informant Indo-European languages Hittite, Vedic Sanskrit, Ancient Greek, and Latin, supplemented by data from Modern Greek.

  16. Georgia : Skills Mismatch and Unemployment, Labor Market Challenges

    World Bank

    2013-01-01

    This paper reviews labor market performance in Georgia, and examines the link between labor market outcomes and poverty. At over 15 percent, the unemployment rate in the country is high by European standards. Georgia faces three main labor market issues: (a) underutilization of labor resources, (b) earnings inequality, and (c) skills mismatch. All three have a negative impact on poverty. T...

  17. Mismatch of Vocational Graduates: What Penalty on French Labour Market?

    Beduwe, Catherine; Giret, Jean-Francois

    2011-01-01

    This study explores individual effects of educational mismatch on wages, job satisfaction and on-the-job-search on French labour market. We distinguish between horizontal matches (job matches with field of studies) and vertical matches (job matches the level of qualification) on the one hand and skills matches (worker's assessment) on the other…

  18. Mismatched feature detection with finer granularity for emotional speaker recognition

    Li CHEN; Ying-chun YANG; Zhao-hui WU

    2014-01-01

    The shapes of speakers’ vocal organs change under their different emotional states, which leads to the deviation of the emotional acoustic space of short-time features from the neutral acoustic space and thereby the degradation of the speaker recognition performance. Features deviating greatly from the neutral acoustic space are considered as mismatched features, and they negatively affect speaker recognition systems. Emotion variation produces different feature deformations for different phonemes, so it is reasonable to build a fi ner model to detect mismatched features under each phoneme. However, given the difficulty of phoneme recognition, three sorts of acoustic class recognition- phoneme classes, Gaussian mixture model (GMM) tokenizer, and probabilistic GMM tokenizer- are proposed to replace phoneme recognition. We propose feature pruning and feature regulation methods to process the mismatched features to improve speaker recognition performance. As for the feature regulation method, a strategy of maximizing the between-class distance and minimizing the within-class distance is adopted to train the transformation matrix to regulate the mismatched features. Experiments conducted on the Mandarin affective speech corpus (MASC) show that our feature pruning and feature regulation methods increase the identifi cation rate (IR) by 3.64% and 6.77%, compared with the baseline GMM-UBM (universal background model) algorithm. Also, corresponding IR increases of 2.09% and 3.32% can be obtained with our methods when applied to the state-of-the-art algorithm i-vector.

  19. Bisimulation Lattice of Asymmetric Chi Calculus with Mismatch

    Dong Xiaoju(董笑菊); Zhong Farong; Fu Yuxi

    2003-01-01

    This paper carries out a systematic investigation into the bisimulation lattice of asymmetric chi calculus with a mismatch combinator. It is shown that all the sixty three L-bisimilarities collapse to twelve distinct relations and they form a bisimulation lattice with respect to set inclusion. The top of the lattice coincides with the barbed bisimilarity.

  20. Clinicopathologic factors identify sporadic mismatch repair-defective colon cancers

    Halvarsson, Britta; Anderson, Harald; Domanska, Katarina; Lindmark, Gudrun; Nilbert, Mef

    2008-01-01

    Identification of sporadic mismatch repair (MMR)-defective colon cancers is increasingly demanded for decisions on adjuvant therapies. We evaluated clinicopathologic factors for the identification of these prognostically favorable tumors. Histopathologic features in 238 consecutive colon cancers...... and excluded 61.5% of the tumors from MMR testing. This clinicopathologic index thus successfully selects MMR-defective colon cancers. Udgivelsesdato: 2008-Feb...

  1. Does the temporal mismatch hypothesis match in boreal populations?

    Vatka, Emma; Rytkönen, Seppo; Orell, Markku

    2014-10-01

    The temporal mismatch hypothesis suggests that fitness is related to the degree of temporal synchrony between the energetic needs of the offspring and their food supply. The hypothesis has been a basis in studying the influence of climate warming on nature. This study enhances the knowledge on prevalence of temporal mismatches and their consequences in boreal populations, and questions the role of the temporal mismatch hypothesis as the principal explanation for the evolution of timing of breeding. To test this, we examined if synchrony with caterpillar prey or timing of breeding per se better explains reproductive output in North European parid populations. We compared responses of temperate-origin species, the great tit (Parus major) and the blue tit (Cyanistes caeruleus), and a boreal species, the willow tit (Poecile montanus). We found that phenologies of caterpillars and great tits, but not of blue tits, have advanced during the past decades. Phenologies correlated with spring temperatures that may function as cues about the timing of the food peak for great and blue tits. The breeding of great and blue tits and their caterpillar food remained synchronous. Synchrony explained breeding success better than timing of breeding alone. However, the synchrony effect arose only in certain conditions, such as with high caterpillar abundances or high breeding densities. Breeding before good synchrony seems advantageous at high latitudes, especially in the willow tit. Thus, the temporal mismatch hypothesis appears insufficient in explaining the evolution of timing of breeding. PMID:25024104

  2. Expectations and Outcomes of Vocational Education: Match or Mismatch.

    Lotto, Linda S.

    1986-01-01

    This article provides an initial look at the matches and mismatches between expectations held for vocational education programs and empirically observed outcomes. Three types of claims for vocational education were compared with recent research. The article concludes with recommendations for improving the alignment between expectations and…

  3. 基于混合居住的城市毗邻隔离住区更新%Renewal of the Urban Adjacent Segregated Residential Area Based on Mixed-Income Residence

    杨昌鸣; 张祥智; 李湘桔

    2013-01-01

    将混合居住理论应用于既有住区更新研究中,在对城市中普遍存在的毗邻隔离住区类型进行概念界定与特征剖析的基础上,提出了互融更新的策略,并尝试在微观层面上深化混合居住理论,增进混合的实效性.%Applying the theory of mixed-income residence to the renewal of existing residential area, and by the concept definition and characteristics analysis, this paper proposes the tactics of mutual fusion for the adjacent and segregated residential area, which is commonly existed in city. It tries to deepen the theory of mixed-income residence at microievel, and to enhance the effectiveness of the mixed-composite.

  4. Band anticrossing effects in highly mismatched semiconductor alloys

    Wu, Junqiao

    2002-09-09

    The first five chapters of this thesis focus on studies of band anticrossing (BAC) effects in highly electronegativity- mismatched semiconductor alloys. The concept of bandgap bowing has been used to describe the deviation of the alloy bandgap from a linear interpolation. Bowing parameters as large as 2.5 eV (for ZnSTe) and close to zero (for AlGaAs and ZnSSe) have been observed experimentally. Recent advances in thin film deposition techniques have allowed the growth of semiconductor alloys composed of significantly different constituents with ever- improving crystalline quality (e.g., GaAs{sub 1-x}N{sub x} and GaP{sub 1-x}N{sub x} with x {approx}< 0.05). These alloys exhibit many novel and interesting properties including, in particular, a giant bandgap bowing (bowing parameters > 14 eV). A band anticrossing model has been developed to explain these properties. The model shows that the predominant bowing mechanism in these systems is driven by the anticrossing interaction between the localized level associated with the minority component and the band states of the host. In this thesis I discuss my studies of the BAC effects in these highly mismatched semiconductors. It will be shown that the results of the physically intuitive BAC model can be derived from the Hamiltonian of the many-impurity Anderson model. The band restructuring caused by the BAC interaction is responsible for a series of experimental observations such as a large bandgap reduction, an enhancement of the electron effective mass, and a decrease in the pressure coefficient of the fundamental gap energy. Results of further experimental investigations of the optical properties of quantum wells based on these materials will be also presented. It will be shown that the BAC interaction occurs not only between localized states and conduction band states at the Brillouin zone center, but also exists over all of k-space. Finally, taking ZnSTe and ZnSeTe as examples, I show that BAC also occurs between

  5. A Statistic-Based Calibration Method for TIADC System

    Kuojun Yang

    2015-01-01

    Full Text Available Time-interleaved technique is widely used to increase the sampling rate of analog-to-digital converter (ADC. However, the channel mismatches degrade the performance of time-interleaved ADC (TIADC. Therefore, a statistic-based calibration method for TIADC is proposed in this paper. The average value of sampling points is utilized to calculate offset error, and the summation of sampling points is used to calculate gain error. After offset and gain error are obtained, they are calibrated by offset and gain adjustment elements in ADC. Timing skew is calibrated by an iterative method. The product of sampling points of two adjacent subchannels is used as a metric for calibration. The proposed method is employed to calibrate mismatches in a four-channel 5 GS/s TIADC system. Simulation results show that the proposed method can estimate mismatches accurately in a wide frequency range. It is also proved that an accurate estimation can be obtained even if the signal noise ratio (SNR of input signal is 20 dB. Furthermore, the results obtained from a real four-channel 5 GS/s TIADC system demonstrate the effectiveness of the proposed method. We can see that the spectra spurs due to mismatches have been effectively eliminated after calibration.

  6. Adjacency effect estimation by ground spectra measurement and satellite optical sensor synchronous observation data

    Jianwen Ma; Xue Chen

    2006-01-01

    @@ This paper addresses the estimation of adjacency effect of CBERS-2 image.The adjacency effect influences the digital number (DN) value of a pixel by adding surrounding scattering signals and path scattering signals.Based on the theory of radiation transfer model,a procedure is designed to measure the reflectance from the surface target materials and the materials in a box,which is 1.5 m above the surface to avoid upwelling reflectance.The results show that the adjacency effect varies from visible,near infrared and becomes steady within short infrared wavelength region;the adjacency effect weakens with the increase of distance between testing sites.The adjacency effect of CBERS-2 image is corrected and the quality of the resulting image is improved.

  7. Achieving good environmental status in the Black Sea: scale mismatches in environmental management

    Tim O'Higgins

    2014-09-01

    Full Text Available The Black Sea has suffered severe environmental degradation. Governance of the Black Sea region is complex and results in a series of scale mismatches which constrain management. This paper develops a simple classification of spatial scale mismatches incorporating the driver, pressure, state, welfare, response (DPSWR framework. The scale mismatch classification is applied to two major environmental problems of the Black Sea, eutrophication and small pelagic fisheries. A number of scale mismatches are described and classified and potential solutions are identified.

  8. Policy Debate | Education and Employment Mismatch

    Mona Mourshed

    2014-12-01

    Full Text Available Editor’s note: These papers are contributions to the ‘Policy Debate’ section of International Development Policy. In this section, academics, policy-makers and practioners engage in a dialogue on global development challenges. Papers are copy-edited but not peer-reviewed. Instead, the initial thematic contribution is followed by critical comments and reactions from scholars and/or policy-makers.Authored by McKinsey’s research team, the initial paper addresses the Education to Employment challenge. It is based on McKinsey’s study, which looked at skill development in 25 different countries and investigated education-to-employment initiatives. The authors claim that the most successful efforts are those where different stakeholders interact intensively and frequently. Employers need to get involved in education, and educators should play a bigger role in employment.The paper is followed by critical comments by two authors, Beatriz Cardoso, Executive Director of Laboratório de Educação, Brazil, and Shailaja Fennell lecturer at the Centre of Development Studies, University of Cambridge, UK.This debate can be pursued on the Journal’s blog http://devpol.hypotheses.org/423.

  9. Rapid identification of a major diffusion/perfusion mismatch in distal internal carotid artery or middle cerebral artery ischemic stroke

    Hakimelahi Reza

    2012-11-01

    Full Text Available Abstract Background We tested the hypothesis that in patients with occlusion of the terminal internal carotid artery and/or the proximal middle cerebral artery, a diffusion abnormality of 70 ml or less is accompanied by a diffusion/perfusion mismatch of at least 100%. Methods Sixty-eight consecutive patients with terminal ICA and/or proximal MCA occlusions and who underwent diffusion/perfusion MRI within 24 hours of stroke onset were retrospectively identified. DWI and mean transit time (MTT volumes were measured. Prospectively, 48 consecutive patients were identified with the same inclusion criteria. DWI and time to peak (TTP lesion volumes were measured. A large mismatch volume was defined as an MTT or TTP abnormality at least twice the DWI lesion volume. Results In the retrospective study, 49 of 68 patients had a DWI lesion volume ≤ 70 ml (mean 20.2 ml; SEM 2.9 ml. A DWI/MTT mismatch of > 100% was observed in all 49 patients (P  100% was present in all 35 (P  Conclusions Acute stroke patients with major anterior circulation artery occlusion are exceedingly likely to have a major diffusion/perfusion mismatch if the diffusion lesion volume is 70 ml or less. This suggests that physiology-based patient assessments may be made using only vessel imaging and diffusion MRI as a simple alternative to perfusion imaging.

  10. Prediction of Steady-State Stresses within Heat Affected Zone Due to Creep Mismatch in Welded Straight Pipes

    This paper reports the steady-state stresses within the heat affected zone (Haz) of a welded straight pipe subject to creep. The creep constants and exponent are varied systematically to see the effect of various mismatches in creep properties on the steady-state creep stresses, via detailed two-dimensional finite element (FE) creep analyses. The weldments consist of the base metal and weld metal with the Haz, which are characterized using the idealized power creep laws with the same creep exponent. The internal pressure and axial loading are considered to see the effect of the loading mode. To quantify the creep stresses, a creep mismatch factor is introduced as a function of the creep constants and exponent. It is concluded that the ratio of the section-averaged stresses for a mismatched case to those for an even matched case are linearly dependent on the mismatch factor. The results are compared with the FE results, including the Type IV region, as well as the R procedure

  11. A Calibrated Charge Pump for Mismatch Reduction in PLL

    Jianhui Wu

    2012-12-01

    Full Text Available An improved charge pump (CP for phase locked loop (PLL applications is presented. The proposed charge pump circuitry employs a variable current source in its sink path, which realizes feedback network for calibration. This scheme of charge pump minimizes mismatch between the sourcing current and the sinking current efficiently. The circuit is simulated in 0.18um CMOS technology and the simulation results show that good current matching characteristics can be achieved. The mismatch between the sourcing current and the sinking current can be reduced to less than 0.01% and the range of charge pump output voltage varies from 0.3V to 1.5V.

  12. Experimental study of proton beam halo in mismatched beams

    We report measurements of transverse beam-halo formation in mismatched proton beams in a 52-quadrupole FODO-transport channel following the 6.7 MeV RFQ at the Low-Energy Demonstration Accelerator (LEDA) at Los Alamos. Beam profiles in both transverse planes were measured using a new diagnostic device that consists of a movable carbon filament for measurement of the beam core, and scraper plates for measurement of the outer part of the distributions. The initial results indicate a surprisingly strong growth rate of the rms emittance even for the modest space-charge tune depressions of the experiment. Our results are consistent with the complete transfer of free energy of the mismatched beams into emittance growth within 10 envelope oscillations for both the breathing and the quadrupole modes.

  13. A new acoustic mismatch theory for Kapitsa resistance

    This paper generalizes the well-known acoustic mismatch theory of Kapitsa interface thermal resistance by taking into consideration a broad class of thermal vibrations that were excluded from that theory by the imposition of the Sommerfeld radiation condition, which is required for the theory of sound but is not relevant for the analysis of heat transport. This extension preserves the main ideas of the acoustic mismatch theory but provides much more reasonable estimates for the interface resistance. The predictions of the new theory are compared with various published experimental results for the thermal resistance between liquid helium at low temperatures and several different metals (Ag, Au, Cu, Pb and Pt). The computations are straightforward and require only well-known material parameters. The predictions agree with the experiments to within their stated range of accuracy.

  14. Graduate Labour Market Mismatches: New Features Of An Older Matter

    Mihai KORKA

    2010-05-01

    Full Text Available In the knowledge society progress depends on innovation, on the knowledge acquired through education, on the science put in practice with the purpose of improving the natural, economic, technical and social condition of human life. Universities are in that favoured position of keeping and creating science, of training through and for science. But, universities face today a series of challenges originated in their relation with the state, with the actors from the national and international extra-university environment, in particular with those active in the labour market. The article explores a variety of education mismatches in the graduate labour market: from over-education to skill mismatches and their impact on the employability. It also focuses on the expected improvement of quality management and of the shared responsibilities for the employability of graduates in the interaction between universities and employers.

  15. Interplay between quantum confinement and dielectric mismatch for ultrashallow dopants

    Mol, J. A.; Salfi, J.; Miwa, J. A.; Simmons, M. Y.; Rogge, S.

    2013-06-01

    Understanding the electronic properties of dopants near an interface is a critical challenge for nanoscale devices. We have determined the effect of dielectric mismatch and quantum confinement on the ionization energy of individual acceptors beneath a hydrogen passivated silicon (100) surface. While dielectric mismatch between the vacuum and the silicon at the interface results in an image charge which enhances the binding energy of subsurface acceptors, quantum confinement is shown to reduce the binding energy. Using scanning tunneling spectroscopy we measure resonant transport through the localized states of individual acceptors. Thermal broadening of the conductance peaks provides a direct measure for the absolute energy scale. Our data unambiguously demonstrates that these two independent effects compete with the result that the ionization energy is less than 5 meV lower than the bulk value for acceptors less than a Bohr radius from the interface.

  16. Three-dimensional visualization of intracranial vessels and adjacent structures

    MR three-dimensional-Fourier-transform, gradient-echo sequences are available that allow the acquisition of high-quality data sets, suitable for three- dimensional image processing. Most recently, flow- compensated measurement sequences are used to achieve a three-dimensional perspective of the vascular anatomy. Spatial relationships between vessels and adjacent structures can be revealed by three-dimensional displays, showing both vessels and tissue surfaces (brain, tumor) in a three- dimensional manner. The image-processing techniques are based on the ray-tracing principle. The method can be used for the optimization of neurosurgical planning in the sellar region

  17. The kernel of the adjacency matrix of a rectangular mesh

    Tomei, Carlos; Vieira, Tania

    2002-01-01

    Given an m x n rectangular mesh, its adjacency matrix A, having only integer entries, may be interpreted as a map between vector spaces over an arbitrary field K. We describe the kernel of A: it is a direct sum of two natural subspaces whose dimensions are equal to $\\lceil c/2 \\rceil$ and $\\lfloor c/2 \\rfloor$, where c = gcd (m+1,n+1) - 1. We show that there are bases to both vector spaces, with entries equal to 0, 1 and -1. When K = Z/(2), the kernel elements of these subspaces are described...

  18. Single-mode quantum properties of the codirectional Kerr nonlinear coupler: frequency mismatch and exact solution

    In this paper, we investigate the single-mode quantum properties of the codirectional Kerr nonlinear coupler when the frequency mismatch is involved and a condition for an exact solution of equations of motion is fulfilled. Particularly, we investigate quadrature and principal squeezing, Wigner function, quadrature distribution, phase distribution and phase variance. We show that the quadrature squeezing and the phase variance can exhibit collapse-revival and collapse-revival-subrevival phenomena, respectively, based on the values of the detuning parameter. Furthermore, we analytically demonstrate that the system can generate cat states, in particular, Yurke-Stoler states

  19. Intraocular/Intracranial pressure mismatch hypothesis for visual impairment syndrome in space.

    Zhang, Li-Fan; Hargens, Alan R

    2014-01-01

    Visual impairment intracranial pressure syndrome (VIIP) is considered a major risk for future human spaceflight. Loss of hydrostatic pressure gradients in vascular and cerebrospinal fluid systems due to the removal of gravity associated with subsequent intracranial and intraocular fluid shifts and the resulting intraocular/intracranial pressure mismatch might be important etiology factors causingVIIP syndrome. Acclimation changes in the ocular and cerebral circulation and the two fluid systems during chronic microgravity exposure and their underlying mechanisms need further elucidation. Relevant findings may help to validate the pressure differential hypothesis for VlIP syndrome and to evaluate whether a gravity based countermeasure is needed. PMID:24479265

  20. Rad9 plays an important role in DNA mismatch repair through physical interaction with MLH1

    He, Wei; Zhao, Yun; Zhang, Chunbo; An, Lili; Hu, Zhishang; Liu, Yuheng; Han, Lu; Bi, Lijun; Xie, Zhensheng; Xue, Peng; Yang, Fuquan; Hang, Haiying

    2008-01-01

    Rad9 is conserved from yeast to humans and plays roles in DNA repair (homologous recombination repair, and base-pair excision repair) and cell cycle checkpoint controls. It has not previously been reported whether Rad9 is involved in DNA mismatch repair (MMR). In this study, we have demonstrated that both human and mouse Rad9 interacts physically with the MMR protein MLH1. Disruption of the interaction by a single-point mutation in Rad9 leads to significantly reduced MMR activity. This disrup...

  1. Robust Feedback Controller for Exponential Stability of Nonlinear Systems with Mismatched Uncertainties

    2002-01-01

    The robust stabilization of nonlinear systems with mismatched uncertainties is investigated. Based on the stability of the nominal system, a new approach to synthesizing a class of continuous state feedback controllers for uncertain nonlinear dynamical systems is proposed. By such feedback controllers, the exponential stability of uncertain nonlinear dynamical systems can be guaranteed. The approach can give a clear insight to system analysis. An illustrative example is given to demonstrate the utilization of the approach developed. Simulation results show that the method presented is practical and effective.

  2. Energy Detection of Multilevel PAM Signals with Systematic Threshold Mismatch

    Antti Anttonen

    2009-01-01

    Full Text Available We address a symbol decision problem with spectrally efficient energy detected multilevel pulse amplitude modulated (PAM signals. First, we analytically quantify the relationship between a systematic threshold mismatch and the required increase of the average signal-to-noise ratio to preserve a desired symbol error rate. For the case in which such an increase is not tolerable, we present a novel near-optimal multilevel threshold selection scheme, which is accurate for a wide range of system parameters.

  3. Race, Segregation, and Postal Employment: New Evidence on Spatial Mismatch

    Leah Platt Boustan; Margo, Robert A.

    2007-01-01

    The spatial mismatch hypothesis posits that employment decentralization isolated urban blacks from work opportunities. This paper focuses on one large employer that has remained in the central city over the twentieth century - the U.S. Postal Service. We find that blacks substitute towards postal work as other employment opportunities leave the city circa 1960. The response is particularly strong in segregated areas, where black neighborhoods are clustered near the central business district. ...

  4. Unconscious learning of auditory discrimination using mismatch negativity (MMN) neurofeedback

    Ming Chang; Hiroyuki Iizuka; Yasushi Naruse; Hideyuki Ando; Taro Maeda

    2014-01-01

    Neurofeedback is a strong direct training method for brain function, wherein brain activity patterns are measured and displayed as feedback, and trainees try to stabilize the feedback signal onto certain desirable states to regulate their own mental states. Here, we introduce a novel neurofeedback method, using the mismatch negativity (MMN) responses elicited by similar sounds that cannot be consciously discriminated. Through neurofeedback training, without participants' attention to the audi...

  5. Education Policy, Occupation-Mismatch and the Skill Premium

    Francesc Obiols-Homs; Virginia Sánchez-Marcos

    2015-01-01

    A relatively low tertiary education wage premium and a large occupational mismatch are two salient features of the Spanish labor market that distinguish it with respect to the labor markets in other developed countries. In this paper we provide an equilibrium model of the labor market with frictions in which workers are heterogeneous in terms of ability and education. We specifically model an education policy as delivering either a particular selection of individuals into the tertiary educati...

  6. Pathological assessment of mismatch repair gene variants in Lynch syndrome

    Rasmussen, Lene Juel; Heinen, Christopher D; Royer-Pokora, Brigitte; Drost, Mark; Tavtigian, Sean; Hofstra, Robert M W; de Wind, Niels

    2012-01-01

    Lynch syndrome (LS) is caused by germline mutations in DNA mismatch repair (MMR) genes and is the most prevalent hereditary colorectal cancer syndrome. A significant proportion of variants identified in MMR and other common cancer susceptibility genes are missense or noncoding changes whose...... attention to common misconceptions about functional assays and endorse development of an integrated approach comprising validated assays for diagnosis of VUS in patients suspected of LS....

  7. Islamic Finance:Structure-objective mismatch and its consequences

    Hasan, Zubair

    2010-01-01

    This paper raises the issue of an initial structure-objective mismatch in the launching of Islamic finance. The abolition of interest and promotion of growth with equity were goals of the conceived system. These goals expressed a long run vision to improve the condition of the Muslim communities across the world. However, the organizational form adopted for Islamic finance was of the existing commercial banks which provided essentially short-term loans on interest to trade industry and commer...

  8. Islamic Finance:Sructure-objective mismatch and its consequences

    Hasan, Zubair

    2010-01-01

    This paper raises the issue of an initial structure-objective mismatch in the launching of Islamic finance. The abolition of interest and promotion of growth with equity were goals of the conceived system. These goals expressed a long run vision to improve the condition of the Muslim communities across the world. However, the organizational form adopted for Islamic finance was of the existing commercial banks which provided essentially short-term loans on interest to trade industry and commer...

  9. Phenotypic mismatches reveal escape from arms-race coevolution.

    Charles T Hanifin

    2008-03-01

    Full Text Available Because coevolution takes place across a broad scale of time and space, it is virtually impossible to understand its dynamics and trajectories by studying a single pair of interacting populations at one time. Comparing populations across a range of an interaction, especially for long-lived species, can provide insight into these features of coevolution by sampling across a diverse set of conditions and histories. We used measures of prey traits (tetrodotoxin toxicity in newts and predator traits (tetrodotoxin resistance of snakes to assess the degree of phenotypic mismatch across the range of their coevolutionary interaction. Geographic patterns of phenotypic exaggeration were similar in prey and predators, with most phenotypically elevated localities occurring along the central Oregon coast and central California. Contrary to expectations, however, these areas of elevated traits did not coincide with the most intense coevolutionary selection. Measures of functional trait mismatch revealed that over one-third of sampled localities were so mismatched that reciprocal selection could not occur given current trait distributions. Estimates of current locality-specific interaction selection gradients confirmed this interpretation. In every case of mismatch, predators were "ahead" of prey in the arms race; the converse escape of prey was never observed. The emergent pattern suggests a dynamic in which interacting species experience reciprocal selection that drives arms-race escalation of both prey and predator phenotypes at a subset of localities across the interaction. This coadaptation proceeds until the evolution of extreme phenotypes by predators, through genes of large effect, allows snakes to, at least temporarily, escape the arms race.

  10. Ballistic Thermal Conduction across Acoustically Mismatched Solid Junctions

    Jian WANG; Wang, Jian-Sheng

    2005-01-01

    We derive expressions for energy flow in terms of lattice normal mode coordinates and energy transmission involving reduced group velocities. With a version of Landauer formula appropriate for lattice dynamic approach, the phonon transmission coefficients and thermal conductance are calculated for two kinds of acoustically mismatched junctions: different chirality nanotubes (11,0) to (8,0), and Si-Ge superlattice structure. Our calculation shows a mode-dependent transmission in nanotube junct...

  11. Towards automatic identification of mismatched image pairs through loop constraints

    Elibol, Armagan; Kim, Jinwhan; Gracias, Nuno; Garcia, Rafael

    2013-12-01

    Obtaining image sequences has become easier and easier thanks to the rapid progress on optical sensors and robotic platforms. Processing of image sequences (e.g., mapping, 3D reconstruction, Simultaneous Localisation and Mapping (SLAM)) usually requires 2D image registration. Recently, image registration is accomplished by detecting salient points in two images and nextmatching their descriptors. To eliminate outliers and to compute a planar transformation (homography) between the coordinate frames of images, robust methods (such as Random Sample Consensus (RANSAC) and Least Median of Squares (LMedS)) are employed. However, image registration pipeline can sometimes provide sufficient number of inliers within the error bounds even when images do not overlap. Such mismatches occur especially when the scene has repetitive texture and shows structural similarity. In this study, we present a method to identify the mismatches using closed-loop (cycle) constraints. The method exploits the fact that images forming a cycle should have identity mapping when all the homographies between images in the cycle multiplied. Cycles appear when the camera revisits an area that was imaged before, which is a common practice especially for mapping purposes. Our proposal extracts several cycles to obtain error statistics for each matched image pair. Then, it searches for image pairs that have extreme error histogram comparing to the other pairs. We present experimental results with artificially added mismatched image pairs on real underwater image sequences.

  12. Infra-red parametric generation: Phase mismatch condition

    An analytical investigation is made for the Infrared parametric generation in doped semiconductor plasma under phase mismatch condition. Theoretical formulations are undertaken to determine induced polarization and threshold pump field for the onset of parametric generation in semiconductor plasma medium. The origin of this nonlinear interaction lies in the second order optical susceptibility arising due to the induced nonlinear current density in piezoelectric medium. Numerical estimations are made for n- type InSb at 77 K duly irradiated by a pulsed 10.6µm CO2 laser. It is very difficult to attain exact phase matching in experimental frame so we have considered a tolerable small phase mismatch in order to attain a new result. Its effect on the Infrared parametric generation in compound semiconductor is examined through induced polarization. Transmitted intensity is determined to have an idea about conversion efficiency of the said process. Phase mismatch tends to raise the required pump field to stimulate the parametric generation. Transmitted intensity is found to decrease with coherence length lc and increase carrier concentration n0, which is favorable for improved conversion efficiency

  13. Review: Clinical aspects of hereditary DNA Mismatch repair gene mutations.

    Sijmons, Rolf H; Hofstra, Robert M W

    2016-02-01

    Inherited mutations of the DNA Mismatch repair genes MLH1, MSH2, MSH6 and PMS2 can result in two hereditary tumor syndromes: the adult-onset autosomal dominant Lynch syndrome, previously referred to as Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the childhood-onset autosomal recessive Constitutional Mismatch Repair Deficiency syndrome. Both conditions are important to recognize clinically as their identification has direct consequences for clinical management and allows targeted preventive actions in mutation carriers. Lynch syndrome is one of the more common adult-onset hereditary tumor syndromes, with thousands of patients reported to date. Its tumor spectrum is well established and includes colorectal cancer, endometrial cancer and a range of other cancer types. However, surveillance for cancers other than colorectal cancer is still of uncertain value. Prophylactic surgery, especially for the uterus and its adnexa is an option in female mutation carriers. Chemoprevention of colorectal cancer with aspirin is actively being investigated in this syndrome and shows promising results. In contrast, the Constitutional Mismatch Repair Deficiency syndrome is rare, features a wide spectrum of childhood onset cancers, many of which are brain tumors with high mortality rates. Future studies are very much needed to improve the care for patients with this severe disorder. PMID:26746812

  14. Semiblind Hyperspectral Unmixing in the Presence of Spectral Library Mismatches

    Fu, Xiao; Ma, Wing-Kin; Bioucas-Dias, Jose M.; Chan, Tsung-Han

    2016-09-01

    The dictionary-aided sparse regression (SR) approach has recently emerged as a promising alternative to hyperspectral unmixing (HU) in remote sensing. By using an available spectral library as a dictionary, the SR approach identifies the underlying materials in a given hyperspectral image by selecting a small subset of spectral samples in the dictionary to represent the whole image. A drawback with the current SR developments is that an actual spectral signature in the scene is often assumed to have zero mismatch with its corresponding dictionary sample, and such an assumption is considered too ideal in practice. In this paper, we tackle the spectral signature mismatch problem by proposing a dictionary-adjusted nonconvex sparsity-encouraging regression (DANSER) framework. The main idea is to incorporate dictionary correcting variables in an SR formulation. A simple and low per-iteration complexity algorithm is tailor-designed for practical realization of DANSER. Using the same dictionary correcting idea, we also propose a robust subspace solution for dictionary pruning. Extensive simulations and real-data experiments show that the proposed method is effective in mitigating the undesirable spectral signature mismatch effects.

  15. Factory waste influence on Elbrus adjacent area

    Complete text of publication follows. Two major burials of waste from concentrating mill of The Tyrnauz Tungsten-Molybdic Plant are situated at the recreation area of Elbrus adjacent territory. But it is significant that the strong winds periods are available here at the river Baksan valley constantly, which are transfers the particles of burials along that valley. The waste pollutes pastures and agricultural lands. The sampling of the buried material on the surface and of the soil of adjacent territory was fulfilled here in order to check up that assumption. The chemical analyses of the buried waste were performed at the X-ray spectrometer Philips PW2400. The results received showed the high quantity of some environmental threat elements. The soil sample analyses on 20 elements (including Ca, Ti, Mn, Fe, P, S, Cr, V, Co, Ni, Cu, Zn, Sr, Zr, Ba, Pb, As, Mo, W, Sn) showed the excess of maximum permissible concentrations (according to Russian Federation hygienic regulations 2.1.7.2041-06) of some investigated elements in the all researched samples. Some results of the investigations are shown below in the table 1. The excesses of maximum permissible concentrations were observed for phosphorus from 5.3 to 11.3 times as much; for sulphur from 3 to 8.75 times as much; for cobalt from 2.6 to 4.6 times as much; for nickel from 8.25 to 15.25 times as much; for copper from 9.3 to 22 times as much; for arsenic up to 10 and at one sample - to 28 times as much. At the MPC level was the lead contents and also at single instances - the manganese and vanadium contents. The excess of background contents were observed for molybdenum up to 8, tungsten - up to 38 and tin - up to 7 times as much. Presented data confirmed the supposition that buried waste pollutes pastures and agricultural lands of Elbrus adjacent area and that means the necessity of either more careful burial of these waste products, or their full recycling.

  16. Seismicity in Azerbaijan and Adjacent Caspian Sea

    So far no general view on the geodynamic evolution of the Black Sea to the Caspian Sea region is elaborated. This is associated with the geological and structural complexities of the region revealed by geophysical, geochemical, petrologic, structural, and other studies. A clash of opinions on geodynamic conditions of the Caucasus region, sometimes mutually exclusive, can be explained by a simplified interpretation of the seismic data. In this paper I analyze available data on earthquake occurrences in Azerbaijan and the adjacent Caspian Sea region. The results of the analysis of macroseismic and instrumental data, seismic regime, and earthquake reoccurrence indicate that a level of seismicity in the region is moderate, and seismic event are concentrated in the shallow part of the lithosphere. Seismicity is mostly intra-plate, and spatial distribution of earthquake epicenters does not correlate with the plate boundaries

  17. Detecting Adjacent Relativity of Engineering Drawing Entities with Container Window

    林福严; 邱友申; 秦吉胜

    2001-01-01

    Automatic recognition and interpretation of engineering drawing plays an important role in computer aided engineering. Detecting the positional relation between entities is an important topic in this research field. In this paper the concepts of adjacent relativity and container window of drawing entities were proposed. By means of container window, the adjacent irrelative entities can be detected quickly and effectively, which speeds up the process of adjacent relativity detection. Meanwhile, the algorithm of adjacent relativity detection was discussed.

  18. Numerical simulations of material mismatch and ductile crack growth

    Oestby, Erling

    2002-07-01

    Both the global geometry and inhomogeneities in material properties will influence the fracture behaviour of structures in presence of cracks. In this thesis numerical simulations have been used to investigate how some aspects of both these issues affect the conditions at the crack-tip. The thesis is organised in an introduction chapter, summarising the major findings and conclusions, a review chapter, presenting the main aspects of the developments in the field of fracture mechanics, and three research papers. Paper I considers the effect of mismatch in hardening exponent on the local near-tip stress field for stationary interface cracks in bi-materials under small scale yielding conditions. It is demonstrated that the stress level in the weaker material increases compared to what is found in the homogeneous material for the same globally applied load level, with the effect being of increasing importance as the crack-tip is approached. Although a coupling between the radial and angular dependence of the stress fields exists, the evolving stress field can still be normalised with the applied J. The effect on the increase in stress level can closely be characterised by the difference in hardening exponent, {delta}n, termed the hardening mismatch, and is more or less independent of the absolute level of hardening in the two materials. Paper II and Ill deal with the effects of geometry, specimen size, hardening level and yield stress mismatch in relation to ductile crack growth. The ductile crack growth is simulated through use of the Gurson model. In Paper H the effect of specimen size on the crack growth resistance is investigated for deep cracked bend and shallow cracked tensile specimens. At small amounts of crack growth the effect of specimen size on the crack growth resistance is small, but a more significant effect is found for larger amounts of crack growth. The crack growth resistance decreases in smaller specimens loaded in tension, whereas the opposite is

  19. Construction of heteroduplex DNA and in vitro model for functional analysis of mismatch repair

    WANG Yi; Clark Alan; WANG Jiaxun; SUN Menghong; SHI Daren

    2004-01-01

    Functional deficiency of mismatch repair (MMR) system is one of the mechanisms of tumorigenesis. With the development of the investigation and the requirement from the clinical diagnosis and treatment it is necessary to build up a method to evaluate the functional status of the whole MMR system in the concerned tumors. The original ssDNA and dsDNA from wild type (wt) bacteriophage M13mp2 and its three derivates with mutation points in the lacZα Gene have been used to construct two kinds of heteroduplex DNA molecules. One named del(2) has two bases deleted in the negative strand, the other has a G·G mismatch base pair in the negative strand too. Introducing this heteroduplex DNA into E. Coli NR9162 (mutS-) without the MMR ability on the indicator plate with x-gal and IPTG, there are three kinds of plaques, mixture plaque as the characteristic phenotype of heteroduplex DNA, blue and clear plaques. If the cell extract is mismatch repair competent the percentage of the mixture plaque will decrease after incubation with these heteroduplex DNA, the repair efficiency is expressed in percentage as 100× (1 minus the ratio of percentages of mixture plaque obtained from the extract-treated sample and untreated samples), which can imply the functional status of MMR system of certain samples. After large T-antigen-dependent SV-40 DNA replication assay cell extract from TK6, a human lymphoblastoid B-cell lymphoma cell line with MMR ability, and Lovo, a human colonic carcinoma cell line with MMR deficiency have incubated with these heteroduplex DNA. The repair efficiency of TK6 to del(2) is more than 60%, to G·G is more than 50%. The Lovo efficiency to del(2) is less than 10%, to G·G is less than 20%. Therefore, in this in vitro model used for functional analysis of mismatch repair of heteroduplex DNA as the repair target, TK6 can serve as the control for MMR proficiency and Lovo as the control for MMR deficiency. Using this model the tumor tissue from a case of hereditary

  20. Study on Ground Motion Attenuation Relation in Shanghai and Its Adjacent Region

    Shi Shuzhong; Shen Jianwen

    2004-01-01

    Based on intensity data in Shanghai and its adjacent region, the intensity attenuation relation is determined. Selecting the western United States as a reference area where there are rich strong ground motion records and intensity data, and by determining ground motion attenuation relation in an area lacking in strong ground motion data, we obtain the ground motion attenuation relation in Shanghai and its adjacent region.

  1. Impact of adjacent land use on coastal wetland sediments.

    Karstens, Svenja; Buczko, Uwe; Jurasinski, Gerald; Peticzka, Robert; Glatzel, Stephan

    2016-04-15

    Coastal wetlands link terrestrial with marine ecosystems and are influenced from both land and sea. Therefore, they are ecotones with strong biogeochemical gradients. We analyzed sediment characteristics including macronutrients (C, N, P, K, Mg, Ca, S) and heavy metals (Mn, Fe, Cu, Zn, Al, Co, Cr, Ni) of two coastal wetlands dominated by Phragmites australis at the Darss-Zingst Bodden Chain, a lagoon system at the Southern Baltic Sea, to identify the impact of adjacent land use and to distinguish between influences from land or sea. In the wetland directly adjacent to cropland (study site Dabitz) heavy metal concentrations were significantly elevated. Fertilizer application led to heavy metal accumulation in the sediments of the adjacent wetland zones. In contrast, at the other study site (Michaelsdorf), where the hinterland has been used as pasture, heavy metal concentrations were low. While the amount of macronutrients was also influenced by vegetation characteristics (e.g. carbon) or water chemistry (e.g. sulfate), the accumulation of heavy metals is regarded as purely anthropogenic influence. A principal component analysis (PCA) based on the sediment data showed that the wetland fringes of the two study sites are not distinguishable, neither in their macronutrient status nor in their concentrations of heavy metals, whereas the interior zones exhibit large differences in terms of heavy metal concentrations. This suggests that seaside influences are minor compared to influences from land. Altogether, heavy metal concentrations were still below national precautionary and action values. However, if we regard the macronutrient and heavy metal concentrations in the wetland fringes as the natural background values, an accumulation of trace elements from agricultural production in the hinterland is apparent. Thus, coastal wetlands bordering croplands may function as effective pollutant buffers today, but the future development has to be monitored closely to avoid

  2. Effect of strength mismatch on fracture mechanical behavior of NG-DMW

    In modern pressurized water reactor (PWR) designs, dissimilar metal joints, e.g. reactor pressure vessel (RPV) safe-ends, are manufactured using a new weld design which takes advantage of narrow-gap (NG) welding technique. In addition to the new weld design, the filler metals have been changed from Alloys 82 and 182 to higher Cr containing Alloys 52 and 152 to ensure the structural integrity of the welds. In dissimilar metal welds (DMW), the mismatch in material properties between the two joined materials and their narrow local variation in different zones of the weld are of importance because the local strength mismatch state plays an important role in the fracture behavior of the weld. For the experimental determination of the local strength variations in a narrow-gap dissimilar metal weld (NG-DMW), a weld mock-up was manufactured using narrow-gap gas-tungsten arc welding (GTAW) method. The weld consisted of SA 508 pressure vessel steel with AISI 309L/308L cladding, AISI 304 piping steel, and Alloy 52 weld metal. The weld was characterized in two different heat treatment conditions, in as-welded condition and in post-weld heat treated (PWHT) condition. The microstructure of the weld mock-up was characterized using FEGSEM. The fusion zone (FZ) between the SA 508 pressure vessel steel and Alloy 52 weld metal was characterized using micro- and nano-hardness testing and the strength mismatch state of the FZ was determined with tensile testing using miniature-sized tensile testing specimens allowing the determination of the local tensile properties of the narrow weld zones near the fusion line (FL). The fracture mechanical testing was performed at room temperature to examine the effect of local strength mismatch on the fracture behavior and crack propagation. The results of the tensile tests revealed that Alloy 52 weld metal had close to equivalent strength with SA 508 base material and the highest strength mismatch existed between the SA 508 heat-affected zone (HAZ

  3. Genome instability of mis-match repair and its role in carcinogenesis due to radiation

    Homologue genes, mutS and mutL have been known as a key gene for mismatch repair in E. coli. In this study, identification of such homologue genes in the nematode was attempted using caenorhabditis elegans and three kinds of mutS homologues (mshG, mshZ and mshF) and 2 kinds of mutL ones were identified. From after isolation of these genes, base sequences were analyzed. Then, an insertion mutant in which Tcl transposon is inserted in Exon 13 positioned at the center of mshF was screened and its homozygote where breakage of transposon in somatic cells occurred frequently was obtained and its morphological changes were not significant. In the nematoda, we detected a highly conserved domain in mutS family gene, which is commonly present in yeast and human genes. Based on the amino acid sequence of this domain, four kinds of primers were constructed for PCR reaction using the whole DNA from the nematoda as a template and four DNA fragments of which sizes were almost corresponding to the homologue proteins were produced. From screening of Tc1 insertion mutant for 8 mismatch repair genes, three strains; mshF, rqhW and RqhY were obtained and the gene structures and the positions of Tc1 insertion in these strains were determined. The sensitivities to ionizing radiation, UV and alkyl reagent of these strains were not significantly different from those of the wild strain. (M.N.)

  4. Mismatch recognition-coupled stabilization of Msh2-Msh6 in an ATP-bound state at the initiation of DNA repair.

    Antony, Edwin; Hingorani, Manju M

    2003-07-01

    Mismatch repair proteins correct errors in DNA via an ATP-driven process. In eukaryotes, the Msh2-Msh6 complex recognizes base pair mismatches and small insertion/deletions in DNA and initiates repair. Both Msh2 and Msh6 proteins contain Walker ATP-binding motifs that are necessary for repair activity. To understand how these proteins couple ATP binding and hydrolysis to DNA binding/mismatch recognition, the ATPase activity of Saccharomyces cerevisiae Msh2-Msh6 was examined under pre-steady-state conditions. Acid-quench experiments revealed that in the absence of DNA, Msh2-Msh6 hydrolyzes ATP rapidly (burst rate = 3 s(-1) at 20 degrees C) and then undergoes a slow step in the pathway that limits catalytic turnover (k(cat) = 0.1 s(-1)). ATP is hydrolyzed similarly in the presence of fully matched duplex DNA; however, in the presence of a G:T mismatch or +T insertion-containing DNA, ATP hydrolysis is severely suppressed (rate = 0.1 s(-1)). Pulse-chase experiments revealed that Msh2-Msh6 binds ATP rapidly in the absence or in the presence of DNA (rate = 0.1 microM(-1) s(-1)), indicating that for the Msh2-Msh6.mismatched DNA complex, a step after ATP binding but before or at ATP hydrolysis is the rate-limiting step in the pathway. Thus, mismatch recognition is coupled to a dramatic increase in the residence time of ATP on Msh2-Msh6. This mismatch-induced, stable ATP-bound state of Msh2-Msh6 likely signals downstream events in the repair pathway. PMID:12820877

  5. Adaptive Neural Stabilizing Controller for a Class of Mismatched Uncertain Nonlinear Systems by State and Output Feedback.

    Arefi, Mohammad Mehdi; Jahed-Motlagh, Mohammad Reza; Karimi, Hamid Reza

    2015-08-01

    In this paper, first, an adaptive neural network (NN) state-feedback controller for a class of nonlinear systems with mismatched uncertainties is proposed. By using a radial basis function NN (RBFNN), a bound of unknown nonlinear functions is approximated so that no information about the upper bound of mismatched uncertainties is required. Then, an observer-based adaptive controller based on RBFNN is designed to stabilize uncertain nonlinear systems with immeasurable states. The state-feedback and observer-based controllers are based on Lyapunov and strictly positive real-Lyapunov stability theory, respectively, and it is shown that the asymptotic convergence of the closed-loop system to zero is achieved while maintaining bounded states at the same time. The presented methods are more general than the previous approaches, handling systems with no restriction on the dimension of the system and the number of inputs. Simulation results confirm the effectiveness of the proposed methods in the stabilization of mismatched nonlinear systems. PMID:25265641

  6. Stress Wave Interaction Between Two Adjacent Blast Holes

    Yi, Changping; Johansson, Daniel; Nyberg, Ulf; Beyglou, Ali

    2016-05-01

    Rock fragmentation by blasting is determined by the level and state of stress in the rock mass subjected to blasting. With the application of electronic detonators, some researchers stated that it is possible to achieve improved fragmentation through stress wave superposition with very short delay times. This hypothesis was studied through theoretical analysis in the paper. First, the stress in rock mass induced by a single-hole shot was analyzed with the assumptions of infinite velocity of detonation and infinite charge length. Based on the stress analysis of a single-hole shot, the stress history and tensile stress distribution between two adjacent holes were presented for cases of simultaneous initiation and 1 ms delayed initiation via stress superposition. The results indicated that the stress wave interaction is local around the collision point. Then, the tensile stress distribution at the extended line of two adjacent blast holes was analyzed for a case of 2 ms delay. The analytical results showed that the tensile stress on the extended line increases due to the stress wave superposition under the assumption that the influence of neighboring blast hole on the stress wave propagation can be neglected. However, the numerical results indicated that this assumption is unreasonable and yields contrary results. The feasibility of improving fragmentation via stress wave interaction with precise initiation was also discussed. The analysis in this paper does not support that the interaction of stress waves improves the fragmentation.

  7. Soil Structure Interaction between Two Adjacent Buildings under Earthquake Load

    Mahmoud Yahyai

    2008-01-01

    Full Text Available In some cases, tall buildings are located in geotechnically unsuitable places, due to their high ratio of height to width; there is risk of uplift and other effects such as overturning and reduction structure serviceability during earthquake. This research is aimed to evaluate the effect of Soil-Structure Interaction (SSI on seismic behavior of two adjacent 32 story buildings such as time period, base shear and displacements. The interaction effects are investigated for variable distance between the two buildings. Three types of soil such as soft clay, sandy gravel and compacted sandy gravel are considered for this study. The result obtained that the interaction effect increases time period of both buildings base shear and lateral displacement of the structures increases.

  8. JOINTING PROCESS OF THE SAE 1020 WITH MAG WELD'S REGION HAVE BEEN INVESTIGATED AND FACTOR OF MISMATCH DETERMINED

    Cevdet MERİÇ

    1997-03-01

    Full Text Available In this study, the jointing process of the SAE 1020 low carbon steel, generally used in the industry, has been done by the MAG weld method. The aim of this study is to examine the mismatch between base and weld metal. After the jointing process, mechanical and metalographical properties of the weld region, HAZ, and the weld metal of the samples considered here were searched, and CTOD (Crack Tip Opening Displacement was identified.

  9. Review of current topics on power cooling mismatch experiments

    A review is made of the current studies of fuel behavior under Power-Cooling-Mismatch (PCM) conditions as part of the light water reactor safety research program. Requirements of research in the field, both in-pile and out-of-pile, are discussed on the basis of the review including the present capability of fuel behavior analysis during a PCM accident, power reactor safety criteria, and experimental results available. Experimental plans in Japan and other countries of PCM research are also described. (auth.)

  10. Triple helix structures: sequence dependence, flexibility and mismatch effects.

    Sun, J S; Mergny, J L; Lavery, R; Montenay-Garestier, T; Hélène, C

    1991-12-01

    By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine. homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen-bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption. PMID:1815635

  11. Identity Management Mismatch Challenges in the Danish Municipality Administration System

    Andersen, Mads Schaarup; Christensen, Henrik Bærbak

    2010-01-01

    municipality administration system, called Opus BRS, a system that integrates SAP, legacy mainframe systems, and other systems present in the individual municipalities. Each of these systems defines their own access control model and architecture, which leads to architectural mismatch that impacts security......Integrating a COTS product in a company’s product portfolio is appealing from a business perspective but highly challenging from the perspective of the software architecture. In this paper we outline research challenges regarding authorization in the identity management part of the Danish...

  12. Mismatch negativity, social cognition, and functional outcomes in patients after traumatic brain injury

    Hui-yan Sun; Qiang Li; Xi-ping Chen; Lu-yang Tao

    2015-01-01

    Mismatch negativity is generated automatically, and is an early monitoring indicator of neuronal integrity impairment and functional abnormality in patients with brain injury, leading to decline of cognitive function. Antipsychotic medication cannot affect mismatch negativity. The present study aimed to explore the relationships of mismatch negativity with neurocognition, daily life and social functional outcomes in patients after brain injury. Twelve patients with traumatic brain injury and 12 healthy controls were recruited in this study. We examined neurocogni-tion with the Wechsler Adult Intelligence Scale-Revised China, and daily and social functional outcomes with the Activity of Daily Living Scale and Social Disability Screening Schedule, re-spectively. Mismatch negativity was analyzed from electroencephalogram recording. The results showed that mismatch negativity amplitudes decreased in patients with traumatic brain injury compared with healthy controls. Mismatch negativity amplitude was negatively correlated with measurements of neurocognition and positively correlated with functional outcomes in patients after traumatic brain injury. Further, the most signiifcant positive correlations were found be-tween mismatch negativity in the fronto-central region and measures of functional outcomes. The most signiifcant positive correlations were also found between mismatch negativity at the FCz electrode and daily living function. Mismatch negativity amplitudes were extremely positive-ly associated with Social Disability Screening Schedule scores at the Fz electrode in brain injury patients. These experimental ifndings suggest that mismatch negativity might efifciently relfect functional outcomes in patients after traumatic brain injury.

  13. Mismatch negativity, social cognition, and functional outcomes in patients after traumatic brain injury

    Hui-yan Sun

    2015-01-01

    Full Text Available Mismatch negativity is generated automatically, and is an early monitoring indicator of neuronal integrity impairment and functional abnormality in patients with brain injury, leading to decline of cognitive function. Antipsychotic medication cannot affect mismatch negativity. The present study aimed to explore the relationships of mismatch negativity with neurocognition, daily life and social functional outcomes in patients after brain injury. Twelve patients with traumatic brain injury and 12 healthy controls were recruited in this study. We examined neurocognition with the Wechsler Adult Intelligence Scale-Revised China, and daily and social functional outcomes with the Activity of Daily Living Scale and Social Disability Screening Schedule, respectively. Mismatch negativity was analyzed from electroencephalogram recording. The results showed that mismatch negativity amplitudes decreased in patients with traumatic brain injury compared with healthy controls. Mismatch negativity amplitude was negatively correlated with measurements of neurocognition and positively correlated with functional outcomes in patients after traumatic brain injury. Further, the most significant positive correlations were found between mismatch negativity in the fronto-central region and measures of functional outcomes. The most significant positive correlations were also found between mismatch negativity at the FCz electrode and daily living function. Mismatch negativity amplitudes were extremely positively associated with Social Disability Screening Schedule scores at the Fz electrode in brain injury patients. These experimental findings suggest that mismatch negativity might efficiently reflect functional outcomes in patients after traumatic brain injury.

  14. Multiple scale model for cell migration in monolayers: Elastic mismatch between cells enhances motility.

    Palmieri, Benoit; Bresler, Yony; Wirtz, Denis; Grant, Martin

    2015-01-01

    We propose a multiscale model for monolayer of motile cells that comprise normal and cancer cells. In the model, the two types of cells have identical properties except for their elasticity; cancer cells are softer and normal cells are stiffer. The goal is to isolate the role of elasticity mismatch on the migration potential of cancer cells in the absence of other contributions that are present in real cells. The methodology is based on a phase-field description where each cell is modeled as a highly-deformable self-propelled droplet. We simulated two types of nearly confluent monolayers. One contains a single cancer cell in a layer of normal cells and the other contains normal cells only. The simulation results demonstrate that elasticity mismatch alone is sufficient to increase the motility of the cancer cell significantly. Further, the trajectory of the cancer cell is decorated by several speed "bursts" where the cancer cell quickly relaxes from a largely deformed shape and consequently increases its translational motion. The increased motility and the amplitude and frequency of the bursts are in qualitative agreement with recent experiments. PMID:26134134

  15. Bromouracil mutagenesis in Escherichia coli evidence for involvement of mismatch repair

    Rydberg, B.

    1977-01-01

    Bromouracil mutagenesis was studied in several strains of E. coli in combination with measurement of incorporation of bromouracil in DNA. For levels below 10% total replacement of bromouracil for thymine, mutagenesis was negligible compared with higher levels of incorporation. Such a nonlinear response occurred both when the bromouracil was evenly distributed over the genome and when a small proportion of the genome was highly substituted. Also, the mutation frequency could be drastically lowered by amino acid starvation following bromouracil incorporation. These observations suggest the involvement of repair phenomena. Studies of mutagenesis in recA/sup -/ and uvrA/sup -/ mutants, as well as studies of prophage induction, did not support an ''error prone'' repair pathway of mutagenesis. On the other hand, uvrD/sup -/ and uvrE/sup -/ mutants, which are deficient in DNA mismatch repair, had much increased mutation frequencies compared with wild type cells. The mutagenic action of bromouracil showed specificity under the conditions used, as demonstrated by the inability of bromouracil to revert an ochre codon that was easily revertable by ultraviolet light irradiation. The results are consistent with a mechanism of bromouracil mutagenesis involvng mispairing, but suggest that the final mutation frequencies depend on repair that removes mismatched bases.

  16. Mismatched single stranded antisense oligonucleotides can induce efficient dystrophin splice switching

    Kole Ryszard

    2011-10-01

    Full Text Available Abstract Background Antisense oligomer induced exon skipping aims to reduce the severity of Duchenne muscular dystrophy by redirecting splicing during pre-RNA processing such that the causative mutation is by-passed and a shorter but partially functional Becker muscular dystrophy-like dystrophin isoform is produced. Normal exons are generally targeted to restore the dystrophin reading frame however, an appreciable subset of dystrophin mutations are intra-exonic and therefore have the potential to compromise oligomer efficiency, necessitating personalised oligomer design for some patients. Although antisense oligomers are easily personalised, it remains unclear whether all patient polymorphisms within antisense oligomer target sequences will require the costly process of producing and validating patient specific compounds. Methods Here we report preclinical testing of a panel of splice switching antisense oligomers, designed to excise exon 25 from the dystrophin transcript, in normal and dystrophic patient cells. These patient cells harbour a single base insertion in exon 25 that lies within the target sequence of an oligomer shown to be effective at removing exon 25. Results It was anticipated that such a mutation would compromise oligomer binding and efficiency. However, we show that, despite the mismatch an oligomer, designed and optimised to excise exon 25 from the normal dystrophin mRNA, removes the mutated exon 25 more efficiently than the mutation-specific oligomer. Conclusion This raises the possibility that mismatched AOs could still be therapeutically applicable in some cases, negating the necessity to produce patient-specific compounds.

  17. Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo.

    Fu, Becky X H; St Onge, Robert P; Fire, Andrew Z; Smith, Justin D

    2016-06-20

    Cas9, a CRISPR-associated RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although complexes between Cas9 and guide RNAs (gRNAs) offer remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the understanding of gRNA::target homology requirements, we compared mutational tolerance for a set of Cas9::gRNA complexes in vitro and in vivo (in Saccharomyces cerevisiae). A variety of gRNAs were tested with variant libraries based on four different targets (with varying GC content and sequence features). In each case, we challenged a mixture of matched and mismatched targets, evaluating cleavage activity on a wide variety of potential target sequences in parallel through high-throughput sequencing of the products retained after cleavage. These experiments evidenced notable and consistent differences between in vitro and S. cerevisiae (in vivo) Cas9 cleavage specificity profiles including (i) a greater tolerance for mismatches in vitro and (ii) a greater specificity increase in vivo with truncation of the gRNA homology regions. PMID:27198218

  18. Predictable patterns of trait mismatches between interacting plants and insects

    Ellis Allan G

    2010-07-01

    Full Text Available Abstract Background There are few predictions about the directionality or extent of morphological trait (mismatches between interacting organisms. We review and analyse studies on morphological trait complementarity (e.g. floral tube length versus insect mouthpart length at the population and species level. Results Plants have consistently more exaggerated morphological traits than insects at high trait magnitudes and in some cases less exaggerated traits than insects at smaller trait magnitudes. This result held at the population level, as well as for phylogenetically adjusted analyses at the species-level and for both pollination and host-parasite interactions, perhaps suggesting a general pattern. Across communities, the degree of trait mismatch between one specialist plant and its more generalized pollinator was related to the level of pollinator specialization at each site; the observed pattern supports the "life-dinner principle" of selection acting more strongly on species with more at stake in the interaction. Similarly, plant mating system also affected the degree of trait correspondence because selfing reduces the reliance on pollinators and is analogous to pollination generalization. Conclusions Our analyses suggest that there are predictable "winners" and "losers" of evolutionary arms races and the results of this study highlight the fact that breeding system and the degree of specialization can influence the outcome.

  19. Mismatch characteristics of optical parametric chirped pulse amplification

    Novák, O.; Turčičová, H.; Divoký, M.; Huynh, J.; Straka, P.

    2014-02-01

    The stability of an optical parametric chirped pulse amplifier (OPCPA) is influenced by time and the angular matching of the input beams. We derived the Gaussian dependence of the monochromatic signal gain on the small mismatch between the signal and pump beams. Gain characteristics were also calculated for polychromatic amplification and the impact of different beam mismatches and interaction geometries was explained. The asymmetry of the energy gain, and the square root dependence of the phase matched wavelength on beam angles were found. The predicted dependences were verified in a noncollinear OPCPA system with LBO and KDP crystal amplifying pulses of a Ti:sapphire laser around a central wavelength of 800 nm, pumped by the third harmonic frequency of an iodine gas laser at a wavelength of 438 nm. The widths of the gain curves in the dependence on both the pump-signal or the phase matching angles varied from several tenths to a few milliradians. The gain curve widths dependent on the pump-signal pulse delay were about two thirds of the pump pulse width for moderate pumping and about a half of the pump pulse width for pumping on the order of GW cm-2. A stable gain output is achieved if angular and temporal fluctuations are fractions of the measured gain curve widths, and when the signal direction is between the pump and the crystal principal axis (i.e. in the psz geometry).

  20. Mismatch repair proficiency is not required for radioenhancement by gemcitabine

    Purpose: Mismatch repair (MMR) proficiency has been reported to either increase or decrease radioenhancement by 24-h incubations with gemcitabine. This study aimed to establish the importance of MMR for radioenhancement by gemcitabine after short-exposure, high-dose treatment and long-exposure, low-dose treatment. Methods and Materials: Survival of MMR-deficient HCT116 and MMR-proficient HCT116 + 3 cells was analyzed by clonogenic assays. Mild, equitoxic gemcitabine treatments (4 h, 0.1 μM vs. 24 h, 6 nM) were combined with γ-irradiation to determine the radioenhancement with or without recovery. Gemcitabine metabolism and cell-cycle effects were evaluated by high-performance liquid chromatography analysis and bivariate flow cytometry. Results: Radioenhancement after 4 h of 0.1 μM of gemcitabine was similar in both cell lines, but the radioenhancement after 24 h of 6 nM of gemcitabine was reduced in MMR-proficient cells. No significant differences between both cell lines were observed in the gemcitabine metabolism or cell-cycle effects after these treatments. Gemcitabine radioenhancement after recovery was also lower in MMR-proficient cells than in MMR-deficient cells. Conclusion: Mismatch repair proficiency decreases radioenhancement by long incubations of gemcitabine but does not affect radioenhancement by short exposures to a clinically relevant gemcitabine dose. Our data suggest that MMR contributes to the recovery from gemcitabine treatment

  1. Mismatch characteristics of optical parametric chirped pulse amplification

    The stability of an optical parametric chirped pulse amplifier (OPCPA) is influenced by time and the angular matching of the input beams. We derived the Gaussian dependence of the monochromatic signal gain on the small mismatch between the signal and pump beams. Gain characteristics were also calculated for polychromatic amplification and the impact of different beam mismatches and interaction geometries was explained. The asymmetry of the energy gain, and the square root dependence of the phase matched wavelength on beam angles were found. The predicted dependences were verified in a noncollinear OPCPA system with LBO and KDP crystal amplifying pulses of a Ti:sapphire laser around a central wavelength of 800 nm, pumped by the third harmonic frequency of an iodine gas laser at a wavelength of 438 nm. The widths of the gain curves in the dependence on both the pump–signal or the phase matching angles varied from several tenths to a few milliradians. The gain curve widths dependent on the pump–signal pulse delay were about two thirds of the pump pulse width for moderate pumping and about a half of the pump pulse width for pumping on the order of GW cm−2. A stable gain output is achieved if angular and temporal fluctuations are fractions of the measured gain curve widths, and when the signal direction is between the pump and the crystal principal axis (i.e. in the psz geometry). (letter)

  2. Counting the mismatches - lung ventilation/perfusion subtraction index

    Full text: There is potential for interobserver variability in interpretation of ventilation/perfusion (V/Q) scans. Objective quantification of V/Q mismatch could be useful. Thus, the aim of this study is to determine the validity of image subtraction in a group of 27 patients (11 men, 8 women; mean age 59.4 years [range 21-81 years])investigated by V/Q scans for suspected pulmonary emboli. A standard 6 view V/Q scan was obtained with two cobalt markers used on the anterior and posterior surfaces for image alignment. Ventilation images were normalised to the perfusion using an area of normal ventilation and perfusion. With the use of automated, and if required, manual alignment, perfusion images were subtracted from ventilation, with a median filter applied. A summed index of mismatch for each lung scan was calculated from the difference. This index was then retrospectively compared to the result reported by one of four experienced physicians. Two patients with chronic obstructive airways disease were excluded from analysis. We conclude that high probability V/Q scans can be differentiated from low probability studies using this index; further prospective investigation in a larger cohort is warranted

  3. Visualization of mismatch repair complexes using fluorescence microscopy.

    Schmidt, Tobias T; Hombauer, Hans

    2016-02-01

    DNA mismatch repair (MMR) is a surveillance mechanism present in most living organisms, which repairs errors introduced by DNA polymerases. Importantly, loss of MMR function due to inactivating mutations and/or epigenetic silencing results in the accumulation of mutations and as consequence increased cancer susceptibility, as observed in Lynch syndrome patients. During the past decades important progress has been made in the MMR field resulting in the identification and characterization of essential MMR components, culminating in the in vitro reconstitution of 5' and 3' nick-directed MMR. However, several mechanistic aspects of the MMR reaction remain not fully understood, therefore alternative approaches and further investigations are needed. Recently, the use of imaging techniques and, more specifically, visualization of MMR components in living cells, has broadened our mechanistic understanding of the repair reaction providing more detailed information about the spatio-temporal organization of MMR in vivo. In this review we would like to comment on mechanistic aspects of the MMR reaction in light of these and other recent findings. Moreover, we will discuss the current limitations and provide future perspectives regarding imaging of mismatch repair components in diverse organisms. PMID:26725956

  4. Halo Formation of a Mismatched Beam in an Induction LINAC

    Chan, Chun Fai

    1996-11-01

    Beam halo formation and emittance growth due to initial envelope mismatch are studied with particle-in-cell simulation for a planned high current experiment with a 2 MeV, 0.8 amp K^+ beam. Transport over 15 m is provided by 55 sets of electrostatic quadrupoles and acceleration could be incorporated with induction modules at drift spaces. The tool used in the study is a (2+1)d code HIBEAM. Clearly visible beam halos are formed in the phase space and real space plots beyond 6 m, if the beam is initially mismatched in the envelope radius and angle by about 20% or more. Furthermore, we present the argument that the physical reason for the effect is due to successive aberration, described earlier by J.D. Lawson.(J.D. Lawson, The Physics of Charged-Particle Beams, p. 200, 1977.) Existing experimental data(M.G. Tiefenback, Proceedings of the 1987 Particle Accelerator Conference, p.1046, 1987.) of halo formation supports this interpretation.

  5. Somatosensory mismatch response in young and elderly adults

    Juho M. Strömmer

    2014-10-01

    Full Text Available Aging is associated with cognitive decline and alterations in early perceptual processes. Studies in the auditory and visual modalities have shown that the mismatch negativity (or the mismatch response, MMR, an event-related potential (ERP elicited by a deviant stimulus in a background of homogenous events, diminishes with aging and cognitive decline. However, the effects of aging on the somatosensory MMR are not known. In the current study, we recorded ERPs to electrical pulses to different fingers of the left hand in a passive oddball experiment in young (22–36 years and elderly (66–95 years adults engaged in a visual task. The MMR was found to deviants as compared to standards at two latency ranges: 180–220 ms and 250–290 ms post-stimulus onset. At 180–220 ms, within the young, the MMR was found at medial electrode sites, whereas aged did not show any amplitude difference between the stimulus types at the same latency range. At 250–290 ms, the MMR was evident with attenuated amplitude and narrowed scalp distribution among aged (Fz compared to young (fronto-centrally and lateral parietal sites. Hence, the results reveal that the somatosensory change detection mechanism is altered in aging. The somatosensory MMR can be used as a reliable measure of age-related changes in sensory-cognitive functions.

  6. Mismatch Limit Load Analyses for V-groove Welded Pipe with Through-wall Circumferential Defect in Centre of Weld

    The present work reports the mismatch limit loads for a V-groove welded pipe for a circumferential crack using finite element (FE) analyses. To integrate the effect of groove angles on mismatch limit loads, one geometry related slenderness parameter was modified by relevant geometric parameters including the groove angle, crack depth, and root opening based on plastic deformation patterns in the theory of plasticity. Circumferential through-wall cracks are located at the centre of the weldments with two different groove angles (45 .deg., 90 .deg.). With regard to the loading conditions, axial (longitudinal) tension and bending are applied for all cases. For the parent and weld metal, elastic-perfectly plastic materials are considered to simulate and analyze under- and over-matching conditions in plasticity. The overall results from the proposed solutions are found to be similar to the FE results

  7. Low-Complexity Estimation of CFO and Frequency Independent I/Q Mismatch for OFDM Systems

    Ying Chen; Jian Zhang; A. D. S. Jayalath

    2009-01-01

    CFO and I/Q mismatch could cause significant performance degradation to OFDM systems. Their estimation and compensation are generally difficult as they are entangled in the received signal. In this paper, we propose some low-complexity estimation and compensation schemes in the receiver, which are robust to various CFO and I/Q mismatch values although the performance is slightly degraded for very small CFO. These schemes consist of three steps: forming a cosine estimator free of I/Q mismatch...

  8. Strong Asymmetric Effect of Lattice Mismatch on Epilayer Structure in Metal Thin Film Deposition

    Hsiao, Pai-Yi; Tsai, Zhuo-Han; Huang, Jia-Hong; Yu, Ge-Ping

    2009-01-01

    We investigate the hetero-epitaxial growth of thin film deposited on a (001) substrate via molecular dynamics simulations, using six fcc transition metals as our modeling systems. By studying the radial distribution function in the film layers, we demonstrate the importance of the sign of lattice mismatch on the layer structure. For positive lattice mismatches, the film favors pseudomorphic growth, whereas for negative mismatches, a sharp transition happens within the first few monolayers of ...

  9. Clinical predictors of prosthesis-patient mismatch after aortic valve replacement for aortic stenosis

    Luis M Astudillo; Orlando Santana; Urbandt, Pablo A.; Benjo, Alexandre M.; Lior U Elkayam; Nascimento, Francisco O.; Lamas, Gervasio A.; Joseph Lamelas

    2012-01-01

    OBJECTIVE: We sought to ascertain predictors of Patient Prosthesis Mismatch, an independent predictor of mortality, in patients with aortic stenosis using bioprosthetic valves. METHOD: We analyzed 2,107 sequential surgeries. Patient Prosthesis Mismatch was calculated using the effective orifice area of the prosthesis divided by the patient's body surface area. We defined nonsignificant, moderate, and severe Patient Prosthesis Mismatch as effective orifice area indexes of >0.85 cm2/m, 0.85-0.6...

  10. Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR

    Ishii, Kousuke; Fukui, Manabu

    2001-01-01

    To reduce PCR bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of PCR products from a mixture of perfect-match and one-mismatch templates. These templates were generated by PCR from Pediococcus acidilactici for one mismatch and Micrococcus luteus for the perfect match. PCRs showed that the bias was reduced at lower temperatures. An environmental sample was also examined.

  11. Creep Rupture of Mismatched Welded Joints of Steels with Dissimilar Creep Strengths

    2001-01-01

    The finite element analysis of mismatched welded joints with a 30°groove angle was performed to study the mechanical behavior of DMWJs (dissimilar metal welded joints). It is concluded that the distribution of stress triaxiality in the DMWJs is uneven, especially near the fusion lines. The degree of creep strength mismatch has remarkable effect on the distribution. The higher the level of mismatch is, the more uneven the distribution is and the easier for premature failure to occur in the joint.

  12. Impact of Time-interleaved Analog-to-Digital Converter Mismatch on Digital Receivers

    Soudan, Michael; Farrell, Ronan

    2008-01-01

    This paper presents the impact that gain, offset and timing mismatch in time-interleaved analog-to-digital converter (TIADC) have on digital receiver systems. An analysis of the mismatch errors shows the dependency of the different errors from the spectrum of the input signal. A discrete-time TIADC model is derived allowing to simulate the mismatch effects of the individual ADCs. Finally, simulations results present the performance degradation that can be expected by the usage of non-id...

  13. Migration of PCBs from Sealants to Adjacent Material

    Andersen, Helle Vibeke; Gunnarsen, Lars Bo; Kampmann, Kristoffer

    2015-01-01

    PCB in sealants can migrate into porous surfaces like concrete and brick and contaminate these materials. The study focuses on an analysis of concentration profiles in relation to alleviation of PCB contamination. The PCB content was determined in 20 samples of concrete and 6 samples of brick...... concentration of the adjacent material. The profiles of PCB concentration into the materials showed some variation, though in general there is a tendency of relative higher concentration near the primary source in concrete and longer migration at low concentrations in brick. The concentration profiles seemed to...... fit an exponential function for concrete and a power function for brick. Based on the sample series, a worst case scenario for migration was defined. For concrete, it showed that a concentration of less than 50 ppm in the material (hazardous waste) was reached at a distance of 5 cm, whereas for bricks...

  14. Mode-mismatched confocal thermal-lens microscope with collimated probe beam

    Cabrera, Humberto, E-mail: hcabrera@ictp.it [SPIE-ICTP Anchor Research Laboratory, International Centre for Theoretical Physics (ICTP), Strada Costiera 11, Trieste (Italy); Centro Multidisciplinartio de Ciencias, Instituto Venezolano de Investigaciones Científicas (IVIC), Mérida 5101 (Venezuela, Bolivarian Republic of); Korte, Dorota; Franko, Mladen [Laboratory for Environmental Research, University of Nova Gorica, Vipavska 13, 5000 Nova Gorica (Slovenia)

    2015-05-15

    We report a thermal lens microscope (TLM) based on an optimized mode-mismatched configuration. It takes advantage of the coaxial counter propagating tightly focused excitation and collimated probe beams, instead of both focused at the sample, as it is in currently known TLM setups. A simple mathematical model that takes into account the main features of the instrument is presented. The confocal detection scheme and the introduction of highly collimated probe beam allow enhancing the versatility, limit of detection (LOD), and sensitivity of the instrument. The theory is experimentally verified measuring ethanol’s absorption coefficient at 532.8 nm. Additionally, the presented technique is applied for detection of ultra-trace amounts of Cr(III) in liquid solution. The achieved LOD is 1.3 ppb, which represents 20-fold enhancement compared to transmission mode spectrometric techniques and a 7.5-fold improvement compared to previously reported methods for Cr(III) based on thermal lens effect.

  15. Mode-mismatched confocal thermal-lens microscope with collimated probe beam

    We report a thermal lens microscope (TLM) based on an optimized mode-mismatched configuration. It takes advantage of the coaxial counter propagating tightly focused excitation and collimated probe beams, instead of both focused at the sample, as it is in currently known TLM setups. A simple mathematical model that takes into account the main features of the instrument is presented. The confocal detection scheme and the introduction of highly collimated probe beam allow enhancing the versatility, limit of detection (LOD), and sensitivity of the instrument. The theory is experimentally verified measuring ethanol’s absorption coefficient at 532.8 nm. Additionally, the presented technique is applied for detection of ultra-trace amounts of Cr(III) in liquid solution. The achieved LOD is 1.3 ppb, which represents 20-fold enhancement compared to transmission mode spectrometric techniques and a 7.5-fold improvement compared to previously reported methods for Cr(III) based on thermal lens effect

  16. PIV Measurements and Mechanisms of Adjacent Synthetic Jets Interactions

    LUO Zhen-Bing; XIA Zhi-Xun

    2008-01-01

    Interactions of adjacent synthetic jet actuators with varying relative amplitude and the relative phase of driving voltage are measured using a particle image velocimetry(PIV).Varying relative amplitude or relative phase of driving voltage of the adjacent actuators vectors the direction of the ensuing merged jet of the adjacent synthetic jets.The vectoring mechanism of the adjacent vortex pairs,attract-impact causing deflection(AICD),is provided to explain why the merged jet is generally vectored to the side of the phase-leading synthetic jet or the synthetic jet with higher driving voltage.

  17. Decentralized Adaptive Control of Systems with Uncertain Interconnections, Plant-Model Mismatch and Actuator Failures

    Patre, Parag; Joshi, Suresh M.

    2011-01-01

    Decentralized adaptive control is considered for systems consisting of multiple interconnected subsystems. It is assumed that each subsystem s parameters are uncertain and the interconnection parameters are not known. In addition, mismatch can exist between each subsystem and its reference model. A strictly decentralized adaptive control scheme is developed, wherein each subsystem has access only to its own state but has the knowledge of all reference model states. The mismatch is estimated online for each subsystem and the mismatch estimates are used to adaptively modify the corresponding reference models. The adaptive control scheme is extended to the case with actuator failures in addition to mismatch.

  18. Application of the simplified J-estimation scheme Aramis to mismatching welds in CCP

    The J-based criteria give reasonable predictions of the failure behaviour of ductile cracked metallic structures, even if the material characterization may be sensitive to the size of the specimens. However in cracked welds, this phenomenon due to stress triaxiality effects could be enhanced. Furthermore, the application of conventional methods of toughness measurement (ESIS or ASTM standard) have evidenced a strong influence of the portion of the weld metal in the specimen. Several authors have shown the inadequacy of the simplified J-estimation methods developed for homogeneous materials. These heterogeneity effects mainly related to the mismatch ratio (ratio of weld metal yield strength upon base metal yield strength) as well as to the geometrical parameter h/W-a (weld width upon ligament size). In order to make decisive progress in this field, the Atomic Energy Commission (CEA), the PWR manufacturer FRAMATOME, and the French utility (EDF) have launched a large research program on cracked piping welds behaviour. As part of this program, a new J-estimation scheme, so called ARAMIS, has been developed to account for the influence of both materials, i.e. base metal and weld metal, on the structural resistance of cracked welds. It has been shown that, when the mismatch is high, and when the ligament size is small compared to the weld width, a classical J-based method using the softer material properties is very conservative. On the opposite the ARAMIS method provides a good estimate of J, because it predicts pretty well the shift of the cracked weld limit load, due to the presence of the weld. the influence of geometrical parameters such as crack size, weld width, or specimen length is property accounted for. (authors). 23 refs., 8 figs., 1 tab., 1 appendix

  19. Effect of weld metal mis-matching on crack driving force

    This paper presents the results of a comparative study on the application of CTOD and J design curves as well as the Engineering Treatment model for Mis-Matched joints (ETM-MM) procedures to mis-matched weld joints. Inhomogeneous through thickness centre-cracked tensile (CCT) panels are prepared by electron beam welding (EBW) a transverse strip of austenitic steel of 18 mm height to the middle of the panel of another austenitic steel of different mechanical properties. The crack tip opening displacement (CTOD) and the experimental J values obtained from such bimaterial panels which simulate the 20 percent undermatched (UM) and 25 percent overmatched (OM) welds are compared with those predicted by the three above-mentioned procedures. Design curves, developed to predict the failure of homogeneous structure, only use the mechanical properties of one material as an input. Moreover, the predictions of such procedures are very sensitive to the mechanical properties and applied strain definitions. Therefore, the significance of the choice of the mechanical properties (weld or base metal properties) to be used in those procedures on the predictions of the crack driving force are discussed. In contrast to the design curves, the recently developed ETM-MM for welded structures includes the mechanical properties of the weld and base metal. Its predictions of the CTOD (δ5) are compared with the experimental results. The mechanical properties of the base or weld metal in the design curves have no significant influence on crack driving force predictions for undermatched specimens. In contrast, for overmatched specimens, the use of mechanical properties of the base metal provides conservative predictions. The predictions obtained using ETM-MM are reasonably conservative for small cracks in an undermatching weld. (orig.)

  20. Twisting right to left: A…A mismatch in a CAG trinucleotide repeat overexpansion provokes left-handed Z-DNA conformation.

    Noorain Khan

    2015-04-01

    Full Text Available Conformational polymorphism of DNA is a major causative factor behind several incurable trinucleotide repeat expansion disorders that arise from overexpansion of trinucleotide repeats located in coding/non-coding regions of specific genes. Hairpin DNA structures that are formed due to overexpansion of CAG repeat lead to Huntington's disorder and spinocerebellar ataxias. Nonetheless, DNA hairpin stem structure that generally embraces B-form with canonical base pairs is poorly understood in the context of periodic noncanonical A…A mismatch as found in CAG repeat overexpansion. Molecular dynamics simulations on DNA hairpin stems containing A…A mismatches in a CAG repeat overexpansion show that A…A dictates local Z-form irrespective of starting glycosyl conformation, in sharp contrast to canonical DNA duplex. Transition from B-to-Z is due to the mechanistic effect that originates from its pronounced nonisostericity with flanking canonical base pairs facilitated by base extrusion, backbone and/or base flipping. Based on these structural insights we envisage that such an unusual DNA structure of the CAG hairpin stem may have a role in disease pathogenesis. As this is the first study that delineates the influence of a single A…A mismatch in reversing DNA helicity, it would further have an impact on understanding DNA mismatch repair.

  1. Diagnostic criteria for constitutional mismatch repair deficiency syndrome

    Wimmer, Katharina; Kratz, Christian P; Vasen, Hans F A;

    2014-01-01

    Constitutional mismatch repair deficiency (CMMRD) syndrome is a distinct childhood cancer predisposition syndrome that results from biallelic germline mutations in one of the four MMR genes, MLH1, MSH2, MSH6 or PMS2. The tumour spectrum is very broad, including mainly haematological, brain and...... intestinal tract tumours. Patients show a variety of non-malignant features that are indicative of CMMRD. However, currently no criteria that should entail diagnostic evaluation of CMMRD exist. We present a three-point scoring system for the suspected diagnosis CMMRD in a paediatric/young adult cancer....... They include multiple hyperpigmented and hypopigmented skin areas, brain malformations, pilomatricomas, a second childhoodmalignancy, a Lynch syndrome (LS)-associated tumour in a relative and parental consanguinity. According to the scoring system, CMMRD should be suspected in any cancer patient who...

  2. Is it time to move mismatch negativity into the clinic?

    Schall, Ulrich

    2016-04-01

    Since its inception in the 1970s, the mismatch negativity (MMN) event-related potential has improved our understanding of pre-attentive detection of rule violations, which is a fundamental cognitive process considered by some a form of "primitive intelligence". The body of research to date ranges from animal studies (i.e. when investigating the neural mechanisms and pharmacological properties of MMN generation) to researching the psychophysiological nature of human consciousness. MMN therefore offers the possibility to detect abnormal functioning in the neural system involved in MMN generation, such as it occurs in some neurodevelopmental disorders or patients in vegetative state. While the clinical research data holds considerable promise for translation into clinical practice, standardization and normative data of an optimized (i.e. disorder-specific) MMN recording algorithm is needed in order for MMN to become a valuable clinical investigation tool. PMID:26342995

  3. Efficient and reproducible identification of mismatch repair deficient colon cancer

    Joost, Patrick; Bendahl, Pär-Ola; Halvarsson, Britta;

    2013-01-01

    BACKGROUND: The identification of mismatch-repair (MMR) defective colon cancer is clinically relevant for diagnostic, prognostic and potentially also for treatment predictive purposes. Preselection of tumors for MMR analysis can be obtained with predictive models, which need to demonstrate ease of...... and efficiently identifies MMR defective colon cancers with high sensitivity and specificity. The model shows stable performance with low inter-observer variability and favorable performance when compared to other MMR predictive models....... application and favorable reproducibility. METHODS: We validated the MMR index for the identification of prognostically favorable MMR deficient colon cancers and compared performance to 5 other prediction models. In total, 474 colon cancers diagnosed ≥ age 50 were evaluated with correlation between...

  4. The mismatch negativity: a review of underlying mechanisms.

    Garrido, Marta I; Kilner, James M; Stephan, Klaas E; Friston, Karl J

    2009-03-01

    The mismatch negativity (MMN) is a brain response to violations of a rule, established by a sequence of sensory stimuli (typically in the auditory domain) [Näätänen R. Attention and brain function. Hillsdale, NJ: Lawrence Erlbaum; 1992]. The MMN reflects the brain's ability to perform automatic comparisons between consecutive stimuli and provides an electrophysiological index of sensory learning and perceptual accuracy. Although the MMN has been studied extensively, the neurophysiological mechanisms underlying the MMN are not well understood. Several hypotheses have been put forward to explain the generation of the MMN; amongst these accounts, the "adaptation hypothesis" and the "model adjustment hypothesis" have received the most attention. This paper presents a review of studies that focus on neuronal mechanisms underlying the MMN generation, discusses the two major explanatory hypotheses, and proposes predictive coding as a general framework that attempts to unify both. PMID:19181570

  5. Microsatellite Instability Use in Mismatch Repair Gene Sequence Variant Classification

    Bryony A. Thompson

    2015-03-01

    Full Text Available Inherited mutations in the DNA mismatch repair genes (MMR can cause MMR deficiency and increased susceptibility to colorectal and endometrial cancer. Microsatellite instability (MSI is the defining molecular signature of MMR deficiency. The clinical classification of identified MMR gene sequence variants has a direct impact on the management of patients and their families. For a significant proportion of cases sequence variants of uncertain clinical significance (also known as unclassified variants are identified, constituting a challenge for genetic counselling and clinical management of families. The effect on protein function of these variants is difficult to interpret. The presence or absence of MSI in tumours can aid in determining the pathogenicity of associated unclassified MMR gene variants. However, there are some considerations that need to be taken into account when using MSI for variant interpretation. The use of MSI and other tumour characteristics in MMR gene sequence variant classification will be explored in this review.

  6. SNP discovery by mismatch-targeting of Mu transposition

    Orsini, Luisa; Pajunen, Maria; Hanski, Ilkka; Savilahti, Harri

    2007-01-01

    Single nucleotide polymorphisms (SNPs) represent a valuable resource for the mapping of human disease genes and induced mutations in model organisms. SNPs may become the markers of choice also for population ecology and evolutionary studies, but their isolation for non-model organisms with unsequenced genomes is often difficult. Here, we describe a rapid and cost-effective strategy to isolate SNPs that exploits the property of the bacteriophage Mu transposition machinery to target mismatched DNA sites and thereby to effectively detect polymorphic loci. To demonstrate the methodology, we isolated 164 SNPs from the unsequenced genome of the Glanville fritillary butterfly (Melitaea cinxia), a much-studied species in population biology, and we validated 24 of them. The strategy involves standard molecular biology techniques as well as undemanding MuA transposase-catalyzed in vitro transposition reactions, and it is applicable to any organism. PMID:17311815

  7. Mismatch Repair and Colon Cancer: Mechanisms and Therapies Explored.

    Li, Stephen K H; Martin, Alberto

    2016-04-01

    Colorectal cancer (CRC) remains one of the most prevalent cancers worldwide. In sporadic CRC, mutations frequently occur in the DNA mismatch repair (MMR) pathway. In addition, germline MMR mutations have been linked to Lynch syndrome, the most common form of hereditary CRC. Although genetic mutations, diet, inflammation, and the gut microbiota can influence CRC, it is unclear how MMR deficiency relates to these factors to modulate disease. In this review, the association of MMR to the etiology of CRC is examined, particularly in the context of microRNAs (miRNAs), inflammation, and the microbiome. We also discuss the most current targeted therapies, methods of prevention, and molecular biomarkers against MMR-deficient CRC, all of which are encouraging advancements in the field. PMID:26970951

  8. Association of common variants in mismatch repair genes and breast cancer susceptibility: a multigene study

    MMR is responsible for the repair of base-base mismatches and insertion/deletion loops. Besides this, MMR is also associated with an anti-recombination function, suppressing homologous recombination. Losses of heterozygosity and/or microsatellite instability have been detected in a large number of skin samples from breast cancer patients, suggesting a potential role of MMR in breast cancer susceptibility. We carried out a hospital-based case-control study in a Caucasian Portuguese population (287 cases and 547 controls) to estimate the susceptibility to non-familial breast cancer associated with some polymorphisms in mismatch repair genes (MSH3, MSH4, MSH6, MLH1, MLH3, PMS1 and MUTYH). Using unconditional logistic regression we found that MLH3 (L844P, G>A) polymorphism GA (Leu/Pro) and AA (Pro/Pro) genotypes were associated with a decreased risk: OR = 0.65 (0.45-0.95) (p = 0.03) and OR = 0.62 (0.41-0.94) (p = 0.03), respectively. Analysis of two-way SNP interaction effects on breast cancer revealed two potential associations to breast cancer susceptibility: MSH3 Ala1045Thr/MSH6 Gly39Glu - AA/TC [OR = 0.43 (0.21-0.83), p = 0.01] associated with a decreased risk; and MSH4 Ala97Thr/MLH3 Leu844Pro - AG/AA [OR = 2.35 (1.23-4.49), p = 0.01], GG/AA [OR = 2.11 (1.12-3,98), p = 0.02], and GG/AG [adjusted OR = 1.88 (1.12-3.15), p = 0.02] all associated with an increased risk for breast cancer. It is possible that some of these common variants in MMR genes contribute significantly to breast cancer susceptibility. However, further studies with a large sample size will be needed to support our results

  9. On spectral hypergraph theory of the adjacency tensor

    Pearson, Kelly J.; Zhang, Tan

    2012-01-01

    We study both $H$ and $E/Z$-eigenvalues of the adjacency tensor of a uniform multi-hypergraph and give conditions for which the largest positive $H$ or $Z$-eigenvalue corresponds to a strictly positive eigenvector. We also investigate when the $E$-spectrum of the adjacency tensor is symmetric.

  10. Study on Optimization of Phase Offset at Adjacent Intersections

    Yuanli GU

    2010-11-01

    Full Text Available Optimization of the phase offset at adjacent intersections is the key parameter regarding coordinated control of traffic signal for adjacent intersections, which decides the effect of the coordinated control for adjacent intersections. According to characters of saturated traffic flow of Chinese urban road, this thesis establishes a model for optimization of phase offset for adjacent interactions and finds a solution from such model by adopting genetic algorithm. The model is verified by actual traffic flow datum of two adjacent signal intersections on Changan Avenue. Then a comparison is made between the optimization result of such model and that of the existing mathematical method and SYNCHRO model, which indicates that the model established by this thesis can reduce the delay suffered by vehicles at the intersections and increase the traffic efficiency of the intersections.

  11. Selecting patients with young-onset colorectal cancer for mismatch repair gene analysis

    Walker, M; O'Sullivan, B; Perakath, B; Taniere, P; Cruger, D; Morton, D

    2007-01-01

    BACKGROUND: Young patients with colorectal cancer are at increased risk of carrying a germline mutation in mismatch repair (MMR) genes. This study investigated the role of clinical criteria and immunohistochemistry for MMR proteins in selecting young patients for mutation testing. METHODS: A cohort...... of 56 consecutive patients with colorectal cancer aged less than 45 years were stratified into three groups based on clinical criteria: 'Amsterdam criteria', 'high risk' and 'young onset only'. Immunohistochemistry for four MMR proteins was carried out and the rate of compliance with clinical...... guidelines determined. RESULTS: Tumours from 11 patients (20 per cent) had abnormal MMR protein expression, of whom eight were referred for genetic assessment. Of 21 patients (38 per cent) in total referred to the genetics unit, six MMR gene mutations were identified, all associated with abnormal...

  12. An Analytical Model for Top-Hat Long Transient Mode-Mismatched Thermal Lens Spectroscopy

    Sabaeian, M.; Rezaei, H.

    2016-02-01

    It has been shown that a top-hat excitation beam gives rise to a more sensitive signal for the thermal lens spectroscopy (TLS). Recently, a numerical model has been presented for a top- hat excitation beam in a dual-beam mod-mismatched TLS [Opt. Lett. 33(13), 1464-1466 (2008)]. In this work, we present a full analytical version of this model. Our model was based on a new solution of time-dependent heat equation for a finite radius cylindrical sample exposed to a top-hat excitation laser beam. The Fresnel diffraction integration method was then used to calculate on-axis probe-beam intensity variations due to thermal lensing by taking the aberrant nature of the thermal lens into account. The model was confirmed with experimental data of LSCAS-2 with an excellent agreement.

  13. Analytical Expressions for Harmonic Distortion at Low Frequencies due to Device Mismatch in CMOS Current Mirrors

    Bruun, Erik

    1999-01-01

    . The expressions are verified through simulations and are used for a discussion of the impact of mismatch on harmonic distortion properties of CMOS current mirrors. The distortion model is combined with well known statistical models for the device mismatch in order to establish a relation between...

  14. Job mismatches and their labour-market effects among school-leavers in Europe

    Wolbers, M.H.J.

    2003-01-01

    In this article, we investigate the determinants of job mismatches with regard to the field of education among school-leavers in Europe. We also examine the effects of job mismatches on the labour-market position of school-leavers. Special attention is paid to cross-national differences in this resp

  15. Educational Mismatch between Graduates' Possessed Skills and Market Demands in Pakistan

    Uzair-ul-Hassan, Muhammad; Noreen, Zahida

    2013-01-01

    Educational mismatch in skills that graduates possess and market requires creates barriers for organizations as well as for job seekers. The study was conducted to find out the educational mismatch between graduates possessed skills and market demands. Convenient sampling was carried out and data were collected from 200 graduates of economics…

  16. Magnesium coordination controls the molecular switch function of DNA mismatch repair protein MutS

    J.H.G. Lebbink (Joyce); A. Fish (Alexander); A. Reumer (Annet); G. Natrajan; H.H.K. Winterwerp (Herrie); T.K. Sixma (Titia)

    2010-01-01

    textabstractThe DNA mismatch repair protein MutS acts as a molecular switch. It toggles between ADP and ATP states and is regulated by mismatched DNA. This is analogous to G-protein switches and the regulation of their "on" and "off" states by guanine exchange factors. Although GDP release in monome

  17. Are Educational Mismatches Responsible for the "Inequality Increasing Effect" of Education?

    Budria, Santiago

    2011-01-01

    This paper asks whether educational mismatches can account for the positive association between education and wage inequality found in the data. We use two different data sources, the European Community Household Panel and the Portuguese Labour Force Survey, and consider several types of mismatch, including overqualification, underqualification…

  18. Scale Mismatches in Social-Ecological Systems: Causes, Consequences, and Solutions

    Charles L. Redman

    2006-06-01

    Full Text Available Scale is a concept that transcends disciplinary boundaries. In ecology and geography, scale is usually defined in terms of spatial and temporal dimensions. Sociological scale also incorporates space and time, but adds ideas about representation and organization. Although spatial and temporal location determine the context for social and ecological dynamics, social-ecological interactions can create dynamic feedback loops in which humans both influence and are influenced by ecosystem processes. We hypothesize that many of the problems encountered by societies in managing natural resources arise because of a mismatch between the scale of management and the scale(s of the ecological processes being managed. We use examples from southern Africa and the southern United States to address four main questions: (1 What is a “scale mismatch?” (2 How are scale mismatches generated? (3 What are the consequences of scale mismatches? (4 How can scale mismatches be resolved? Scale mismatches occur when the scale of environmental variation and the scale of social organization in which the responsibility for management resides are aligned in such a way that one or more functions of the social-ecological system are disrupted, inefficiencies occur, and/or important components of the system are lost. They are generated by a wide range of social, ecological, and linked social-ecological processes. Mismatches between the scales of ecological processes and the institutions that are responsible for managing them can contribute to a decrease in social-ecological resilience, including the mismanagement of natural resources and a decrease in human well-being. Solutions to scale mismatches usually require institutional changes at more than one hierarchical level. Long-term solutions to scale mismatch problems will depend on social learning and the development of flexible institutions that can adjust and reorganize in response to changes in ecosystems. Further research

  19. Delayed acquisition of non-adjacent vocalic distributional regularities.

    Gonzalez-Gomez, Nayeli; Nazzi, Thierry

    2016-01-01

    The ability to compute non-adjacent regularities is key in the acquisition of a new language. In the domain of phonology/phonotactics, sensitivity to non-adjacent regularities between consonants has been found to appear between 7 and 10 months. The present study focuses on the emergence of a posterior-anterior (PA) bias, a regularity involving two non-adjacent vowels. Experiments 1 and 2 show that a preference for PA over AP (anterior-posterior) words emerges between 10 and 13 months in French-learning infants. Control experiments show that this bias cannot be explained by adjacent or positional preferences. The present study demonstrates that infants become sensitive to non-adjacent vocalic distributional regularities between 10 and 13 months, showing the existence of a delay for the acquisition of non-adjacent vocalic regularities compared to equivalent non-adjacent consonantal regularities. These results are consistent with the CV hypothesis, according to which consonants and vowels play different roles at different linguistic levels. PMID:25786491

  20. Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast.

    Gamble, Caitlin E; Brule, Christina E; Dean, Kimberly M; Fields, Stanley; Grayhack, Elizabeth J

    2016-07-28

    Translation elongation efficiency is largely thought of as the sum of decoding efficiencies for individual codons. Here, we find that adjacent codon pairs modulate translation efficiency. Deploying an approach in Saccharomyces cerevisiae that scored the expression of over 35,000 GFP variants in which three adjacent codons were randomized, we have identified 17 pairs of adjacent codons associated with reduced expression. For many pairs, codon order is obligatory for inhibition, implying a more complex interaction than a simple additive effect. Inhibition mediated by adjacent codons occurs during translation itself as GFP expression is restored by increased tRNA levels or by non-native tRNAs with exact-matching anticodons. Inhibition operates in endogenous genes, based on analysis of ribosome profiling data. Our findings suggest translation efficiency is modulated by an interplay between tRNAs at adjacent sites in the ribosome and that this concerted effect needs to be considered in predicting the functional consequences of codon choice. PMID:27374328

  1. Uplink scheduling and adjacent-channel coupling loss analysis for TD-LTE deployment.

    Yeo, Woon-Young; Moon, Sung Ho; Kim, Jae-Hoon

    2014-01-01

    TD-LTE, one of the two duplexing modes in LTE, operates in unpaired spectrum and has the advantages of TDD-based technologies. It is expected that TD-LTE will be more rapidly deployed in near future and most of WiMax operators will upgrade their networks to TD-LTE gradually. Before completely upgrading to TD-LTE, WiMax may coexist with TD-LTE in an adjacent frequency band. In addition, multiple TD-LTE operators may deploy their networks in adjacent bands. When more than one TDD network operates in adjacent frequency bands, severe interference may happen due to adjacent channel interference (ACI) and unsynchronized operations. In this paper, coexistence issues between TD-LTE and other systems are analyzed and coexistence requirements are provided. This paper has three research objectives. First, frame synchronization between TD-LTE and WiMax is discussed by investigating possible combinations of TD-LTE and WiMax configurations. Second, an uplink scheduling algorithm is proposed to utilize a leakage pattern of ACI in synchronized operations. Third, minimum requirements for coexistence in unsynchronized operations are analyzed by introducing a concept of adjacent-channel coupling loss. From the analysis and simulation results, we can see that coexistence of TD-LTE with other TDD systems is feasible if the two networks are synchronized. For the unsynchronized case, some special cell-site engineering techniques may be required to reduce the ACI. PMID:24707214

  2. Identification of protein-RNA interaction sites using the information of spatial adjacent residues

    Cheng Yong-Mei

    2011-10-01

    Full Text Available Abstract Background Protein-RNA interactions play an important role in numbers of fundamental cellular processes such as RNA splicing, transport and translation, protein synthesis and certain RNA-mediated enzymatic processes. The more knowledge of Protein-RNA recognition can not only help to understand the regulatory mechanism, the site-directed mutagenesis and regulation of RNA–protein complexes in biological systems, but also have a vitally effecting for rational drug design. Results Based on the information of spatial adjacent residues, novel feature extraction methods were proposed to predict protein-RNA interaction sites with SVM-KNN classifier. The total accuracies of spatial adjacent residue profile feature and spatial adjacent residues weighted accessibility solvent area feature are 78%, 67.07% respectively in 5-fold cross-validation test, which are 1.4%, 3.79% higher than that of sequence neighbour residue profile feature and sequence neighbour residue accessibility solvent area feature. Conclusions The results indicate that the performance of feature extraction method using the spatial adjacent information is superior to the sequence neighbour information approach. The performance of SVM-KNN classifier is little better than that of SVM. The feature extraction method of spatial adjacent information with SVM-KNN is very effective for identifying protein-RNA interaction sites and may at least play a complimentary role to the existing methods.

  3. Computation and analysis of the geomagnetic field model in China and its adjacent area for 2003

    GU Zuo-wen; AN Zhen-chang; GAO Jin-tian; ZHAN Zhi-jia; YAO Tong-qi; HAN Wei; CHEN Bin

    2006-01-01

    Based on the geomagnetic data at 135 stations and 35 observatories in China in 2003, the Taylor polynomial model and the spherical cap harmonic model in China and its adjacent area for 2003 were established. In the model calculation, the truncation order of the model and the influences of the boundary restriction on the model calculation were carefully analyzed. The results show that the geomagnetic data used are precise and reliable, and the selection of the truncation order is reasonable. The Taylor polynomial model and the spherical cap harmonic model in China and its adjacent area established in this paper are consistent very well.

  4. Interactions of adjacent pulsating, erupting and creeping solitons

    Song Li-Jun; Li Lu; Zhou Guo-Sheng

    2007-01-01

    This paper investigates the adjacent interactions of three novel solitons for the quintic complex Ginzburg-Landau equation, which are plain pulsating, erupting and creeping solitons. It is found that different performances are presented for different solitons due to isolated regions of the parameter space where they exist. For example, plain pulsating and erupting solitons exhibit mutual annihilation during collisions with the decrease of total energy, but for creeping soliton,the two adjacent pulses present soliton fusion without any loss of energy. Otherwise, the method for restraining the interactions is also found and it can suppress interacions between these two adjacent pulses effectively.

  5. Griset: Pottery of the Great Basin and Adjacent Areas

    Forsyth, Donald W.

    1986-01-01

    Pottery of the Great Basin and Adjacent Areas. Suzanne Griset, ed. University of Utah Anthropological Papers No. Ill, 1986, 170 pp., 34 figs., 5 tables, annotated and indexed bibliography, $17.50 (paper).

  6. The Visual Mismatch Negativity Elicited with Visual Speech Stimuli

    Lynne E Bernstein

    2013-07-01

    Full Text Available The visual mismatch negativity (vMMN, deriving from the brain’s response to stimulus deviance, is thought to be generated by the cortex that represents the stimulus. The vMMN response to visual speech stimuli was used in a study of the lateralization of visual speech processing. Previous research suggested that the right posterior temporal cortex has specialization for processing simple non-speech face gestures, and the left posterior temporal cortex has specialization for processing visual speech gestures. Here, visual speech consonant-vowel (CV stimuli with controlled perceptual dissimilarities were presented in an electroencephalography (EEG vMMN paradigm. The vMMNs were obtained using the comparison of event-related potentials (ERPs for separate CVs in their roles as deviant versus their roles as standard. Four separate vMMN contrasts were tested, two with the perceptually far deviants (i.e., “zha” or “fa” and two with the near deviants (i.e., “zha” or “ta”. Only far deviants evoked the vMMN response over the left posterior temporal cortex. All four deviants evoked vMMNs over the right posterior temporal cortex. The results are interpreted as evidence that the left posterior temporal cortex represents speech stimuli that are perceived as different consonants, and the right posterior temporal cortex represents face gestures that may not be discriminable as different CVs.

  7. MCM9 Is Required for Mammalian DNA Mismatch Repair.

    Traver, Sabine; Coulombe, Philippe; Peiffer, Isabelle; Hutchins, James R A; Kitzmann, Magali; Latreille, Daniel; Méchali, Marcel

    2015-09-01

    DNA mismatch repair (MMR) is an evolutionarily conserved process that corrects DNA polymerase errors during replication to maintain genomic integrity. In E. coli, the DNA helicase UvrD is implicated in MMR, yet an analogous helicase activity has not been identified in eukaryotes. Here, we show that mammalian MCM9, a protein involved in replication and homologous recombination, forms a complex with MMR initiation proteins (MSH2, MSH3, MLH1, PMS1, and the clamp loader RFC) and is essential for MMR. Mcm9-/- cells display microsatellite instability and MMR deficiency. The MCM9 complex has a helicase activity that is required for efficient MMR since wild-type but not helicase-dead MCM9 restores MMR activity in Mcm9-/- cells. Moreover, MCM9 loading onto chromatin is MSH2-dependent, and in turn MCM9 stimulates the recruitment of MLH1 to chromatin. Our results reveal a role for MCM9 and its helicase activity in mammalian MMR. PMID:26300262

  8. A frontal attention mechanism in the visual mismatch negativity

    Hedge, Craig; Stothart, George; Todd Jones, Jenna; Rojas Frías, Priscila; Magee, Kristopher Lundy; Brooks, Jonathan C.W.

    2015-01-01

    Automatic detection of environmental change is a core component of attention. The mismatch negativity (MMN), an electrophysiological marker of this mechanism, has been studied prominently in the auditory domain, with cortical generators identified in temporal and frontal regions. Here, we combined electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) to assess whether the underlying frontal regions associated with auditory change detection also play a role in visual change detection. Twenty healthy young adults completed a visual MMN task in separate EEG and fMRI sessions. Region of interest analyses were conducted on left and right middle frontal (MFG) and inferior frontal (IFG) gyri, i.e., the frontal areas identified as potential auditory MMN generators. A significant increase in activation was observed in the left IFG and MFG in response to blocks containing deviant stimuli. These findings suggest that a frontal mechanism is involved in the detection of change in the visual MMN. Our results support the notion that frontal mechanisms underlie attention switching, as measured via MMN, across multiple modalities. PMID:26183650

  9. Integrated analysis of mismatch repair system in malignant astrocytomas.

    Irene Rodríguez-Hernández

    Full Text Available Malignant astrocytomas are the most aggressive primary brain tumors with a poor prognosis despite optimal treatment. Dysfunction of mismatch repair (MMR system accelerates the accumulation of mutations throughout the genome causing uncontrolled cell growth. The aim of this study was to characterize the MMR system defects that could be involved in malignant astrocytoma pathogenesis. We analyzed protein expression and promoter methylation of MLH1, MSH2 and MSH6 as well as microsatellite instability (MSI and MMR gene mutations in a set of 96 low- and high-grade astrocytomas. Forty-one astrocytomas failed to express at least one MMR protein. Loss of MSH2 expression was more frequent in low-grade astrocytomas. Loss of MLH1 expression was associated with MLH1 promoter hypermethylation and MLH1-93G>A promoter polymorphism. However, MSI was not related with MMR protein expression and only 5% of tumors were MSI-High. Furthermore, the incidence of tumors carrying germline mutations in MMR genes was low and only one glioblastoma was associated with Lynch syndrome. Interestingly, survival analysis identified that tumors lacking MSH6 expression presented longer overall survival in high-grade astrocytoma patients treated only with radiotherapy while MSH6 expression did not modify the prognosis of those patients treated with both radiotherapy and chemotherapy. Our findings suggest that MMR system alterations are a frequent event in malignant astrocytomas and might help to define a subgroup of patients with different outcome.

  10. Snowshoe hares display limited phenotypic plasticity to mismatch in seasonal camouflage

    Zimova, Marketa; Mills, L. Scott; Lukacs, Paul M.; Mitchell, Michael S.

    2014-01-01

    As duration of snow cover decreases owing to climate change, species undergoing seasonal colour moults can become colour mismatched with their background. The immediate adaptive solution to this mismatch is phenotypic plasticity, either in phenology of seasonal colour moults or in behaviours that reduce mismatch or its consequences. We observed nearly 200 snowshoe hares across a wide range of snow conditions and two study sites in Montana, USA, and found minimal plasticity in response to mismatch between coat colour and background. We found that moult phenology varied between study sites, likely due to differences in photoperiod and climate, but was largely fixed within study sites with only minimal plasticity to snow conditions during the spring white-to-brown moult. We also found no evidence that hares modify their behaviour in response to colour mismatch. Hiding and fleeing behaviours and resting spot preference of hares were more affected by variables related to season, site and concealment by vegetation, than by colour mismatch. We conclude that plasticity in moult phenology and behaviours in snowshoe hares is insufficient for adaptation to camouflage mismatch, suggesting that any future adaptation to climate change will require natural selection on moult phenology or behaviour.

  11. Measurement errors in multifrequency bioelectrical impedance analyzers with and without impedance electrode mismatch

    The purpose of this study is to compare measurement errors in two commercially available multi-frequency bioimpedance analyzers, a Xitron 4000B and an ImpediMed SFB7, including electrode impedance mismatch. The comparison was made using resistive electrical models and in ten human volunteers. We used three different electrical models simulating three different body segments: the right-side, leg and thorax. In the electrical models, we tested the effect of the capacitive coupling of the patient to ground and the skin–electrode impedance mismatch. Results showed that both sets of equipment are optimized for right-side measurements and for moderate skin–electrode impedance mismatch. In right-side measurements with mismatch electrode, 4000B is more accurate than SFB7. When an electrode impedance mismatch was simulated, errors increased in both bioimpedance analyzers and the effect of the mismatch in the voltage detection leads was greater than that in current injection leads. For segments with lower impedance as the leg and thorax, SFB7 is more accurate than 4000B and also shows less dependence on electrode mismatch. In both devices, impedance measurements were not significantly affected (p > 0.05) by the capacitive coupling to ground

  12. Quality of Experience for Large Ultra-High-Resolution Tiled Displays with Synchronization Mismatch

    Deshpande Sachin

    2011-01-01

    Full Text Available This paper relates to quality of experience when viewing images, video, or other content on large ultra-high-resolution displays made from individual display tiles. We define experiments to measure vernier acuity caused by synchronization mismatch for moving images. The experiments are used to obtain synchronization mismatch acuity threshold as a function of object velocity and as a function of occlusion or gap width. Our main motivation for measuring the synchronization mismatch vernier acuity is its relevance in the application of tiled display systems, which create a single contiguous image using individual discrete panels arranged in a matrix with each panel utilizing a distributed synchronization algorithm to display parts of the overall image. We also propose a subjective assessment method for perception evaluation of synchronization mismatch for large ultra-high-resolution tiled displays. For this, we design a synchronization mismatch measurement test video set for various tile configurations for various interpanel synchronization mismatch values. The proposed method for synchronization mismatch perception can evaluate tiled displays with or without tile bezels. The results from this work can help during design of low-cost tiled display systems, which utilize distributed synchronization mechanisms for a contiguous or bezeled image display.

  13. Structural studies of the 5'-phenazinium-tethered matched and G-A-mismatched DNA duplexes by NMR spectroscopy.

    Maltseva, T; Sandström, A; Ivanova, I M; Sergeyev, D S; Zarytova, V F; Chattopadhyaya, J

    1993-05-01

    The mechanism through which modified oligo-DNA analogues act as antisense repressors at the transcriptional and translational level of gene expression is based on the information content in the nucleotide sequence which is determined by the specific base pairing. The efficiency of such action is largely determined by the stability of the duplex formed between the oligonucleotide reagent and the target sequence and also by the mismatched base pairing, such as G-A, that occurs during replication or recombination. We herein report that the phenazinium (Pzn)-tethered matched duplex p(d(TGTTTGGC)):(Pzn)-p(d(CCAAACA)) (III) (Tm = 50 degrees C) has a much larger stability than the parent matched duplex p(d(TGTTTGGC)):p(d(CCAAACA)) (I) (Tm = 30 degrees C). On the other hand, the Pzn-tethered G-A-mismatched duplex p(d(TGTTTGGC)):(Pzn)-p(d(ACAAACA)) (IV) (Tm = 34 degrees C) is only slightly more stable than its parent mismatched duplex p(d(TGTTTGGC)):p(d(ACAAACA)) (Tm = 25 degrees C). A detailed 500 MHz NMR study and constrained MD refinements of NMR-derived structures have been undertaken for the DNA duplexes (I), (II), (III) and (IV) in order to understand the structural basis of stabilization of Pzn-tethered matched DNA duplex (delta Tm = 20 degrees C) compared to mismatched duplex (delta Tm = 9 degrees C). Assignment of the 1H-NMR (500 MHz) spectra of the duplexes has been carried out by 2D NOESY, HOHAHA and DQF-COSY experiments. The torsion angles have been extracted from the J-coupling constants obtained by simulation of most of the DQF-COSY cross-peaks using program SMART. The solution structure of the duplexes were assessed by an iterative hybride relaxation matrix method (MORASS) combined with NOESY distances and torsion angles restrained molecular dynamics (MD) using program Amber 4.0. The standard Amber 4.0 force-field parameters were used for the oligonucleotide in conjunction with the new parameters for Pzn residue which was obtained by full geometry

  14. A deterministic seismic hazard map of India and adjacent areas

    A seismic hazard map of the territory of India and adjacent areas has been prepared using a deterministic approach based on the computation of synthetic seismograms complete of all main phases. The input data set consists of structural models, seismogenic zones, focal mechanisms and earthquake catalogue. The synthetic seismograms have been generated by the modal summation technique. The seismic hazard, expressed in terms of maximum displacement (DMAX), maximum velocity (VMAX), and design ground acceleration (DGA), has been extracted from the synthetic signals and mapped on a regular grid of 0.2 deg. x 0.2 deg. over the studied territory. The estimated values of the peak ground acceleration are compared with the observed data available for the Himalayan region and found in good agreement. Many parts of the Himalayan region have the DGA values exceeding 0.6 g. The epicentral areas of the great Assam earthquakes of 1897 and 1950 represent the maximum hazard with DGA values reaching 1.2-1.3 g. (author)

  15. Characterization of adjacent breast tumors using oligonucleotide microarrays

    Current methodology often cannot distinguish second primary breast cancers from multifocal disease, a potentially important distinction for clinical management. In the present study we evaluated the use of oligonucleotide-based microarray analysis in determining the clonality of tumors by comparing gene expression profiles. Total RNA was extracted from two tumors with no apparent physical connection that were located in the right breast of an 87-year-old woman diagnosed with invasive ductal carcinoma (IDC). The RNA was hybridized to the Affymetrix Human Genome U95A Gene Chip® (12,500 known human genes) and analyzed using the Gene Chip Analysis Suite® 3.3 (Affymetrix, Inc, Santa Clara, CA, USA) and JMPIN® 3.2.6 (SAS Institute, Inc, Cary, NC, USA). Gene expression profiles of tumors from five additional patients were compared in order to evaluate the heterogeneity in gene expression between tumors with similar clinical characteristics. The adjacent breast tumors had a pairwise correlation coefficient of 0.987, and were essentially indistinguishable by microarray analysis. Analysis of gene expression profiles from different individuals, however, generated a pairwise correlation coefficient of 0.710. Transcriptional profiling may be a useful diagnostic tool for determining tumor clonality and heterogeneity, and may ultimately impact on therapeutic decision making

  16. Concentric network symmetry grasps authors' styles in word adjacency networks

    Amancio, Diego R.; Silva, Filipi N.; Costa, Luciano da F.

    2015-06-01

    Several characteristics of written texts have been inferred from statistical analysis derived from networked models. Even though many network measurements have been adapted to study textual properties at several levels of complexity, some textual aspects have been disregarded. In this paper, we study the symmetry of word adjacency networks, a well-known representation of text as a graph. A statistical analysis of the symmetry distribution performed in several novels showed that most of the words do not display symmetric patterns of connectivity. More specifically, the merged symmetry displayed a distribution similar to the ubiquitous power-law distribution. Our experiments also revealed that the studied metrics do not correlate with other traditional network measurements, such as the degree or the betweenness centrality. The discriminability power of the symmetry measurements was verified in the authorship attribution task. Interestingly, we found that specific authors prefer particular types of symmetric motifs. As a consequence, the authorship of books could be accurately identified in 82.5% of the cases, in a dataset comprising books written by 8 authors. Because the proposed measurements for text analysis are complementary to the traditional approach, they can be used to improve the characterization of text networks, which might be useful for applications based on stylistic classification.

  17. The Thermomagnetic Instability in Superconducting Films with Adjacent Metal Layer

    Vestgården, J. I.; Galperin, Y. M.; Johansen, T. H.

    2013-12-01

    Dendritic flux avalanches is a frequently encountered consequence of the thermomagnetic instability in type-II superconducting films. The avalanches, which are potentially harmful for superconductor-based devices, can be suppressed by an adjacent normal metal layer, even when the two layers are not in thermal contact. The suppression of the avalanches in this case is due to so-called magnetic braking, caused by eddy currents generated in the metal layer by propagating magnetic flux. We develop a theory of magnetic braking by analyzing coupled electrodynamics and heat flow in a superconductor-normal metal bilayer. The equations are solved by linearization and by numerical simulation of the avalanche dynamics. We find that in an uncoated superconductor, even a uniform thermomagnetic instability can develop into a dendritic flux avalanche. The mechanism is that a small non-uniformity caused by the electromagnetic non-locality induces a flux-flow hot spot at a random position. The hot spot quickly develops into a finger, which at high speeds penetrates into the superconductor, forming a branching structure. Magnetic braking slows the avalanches, and if the normal metal conductivity is sufficiently high, it can suppress the formation of the dendritic structure. During avalanches, the braking by the normal metal layer prevents the temperature from exceeding the transition temperature of the superconductor. Analytical criteria for the instability threshold are developed using the linear stability analysis. The criteria are found to match quantitatively the instability onsets obtained in simulations.

  18. The UvrD helicase and its modulation by the mismatch repair protein MutL

    Matson, Steven W.; Robertson, Adam B.

    2006-01-01

    UvrD is a superfamily I DNA helicase with well documented roles in excision repair and methyl-directed mismatch repair (MMR) in addition to poorly understood roles in replication and recombination. The MutL protein is a homodimeric DNA-stimulated ATPase that plays a central role in MMR in Escherichia coli. This protein has been characterized as the master regulator of mismatch repair since it interacts with and modulates the activity of several other proteins involved in the mismatch repair p...

  19. The origins and early evolution of DNA mismatch repair genes—multiple horizontal gene transfers and co-evolution

    Lin, Zhenguo; Nei, Masatoshi; Ma, Hong

    2007-01-01

    To understand the evolutionary process of the DNA mismatch repair system, we conducted systematic phylogenetic analysis of its key components, the bacterial MutS and MutL genes and their eukaryotic homologs. Based on genome-wide homolog searches, we identified three new MutS subfamilies (MutS3-5) in addition to the previously studied MutS1 and MutS2 subfamilies. Detailed evolutionary analysis strongly suggests that frequent ancient horizontal gene transfer (HGT) occurred with both MutS and Mu...

  20. The speciation of marine particulate iron adjacent to active and passive continental margins

    Lam, Phoebe J.; Ohnemus, Daniel C.; Marcus, Matthew A.

    2012-03-01

    We use synchrotron-based chemical-species mapping techniques to compare the speciation of suspended (1-51 μm) marine particulate iron collected in two open ocean environments adjacent to active and passive continental margins. Chemical-species mapping provides speciation information for heterogeneous environmental samples, and is especially good for detecting spectroscopically distinct trace minerals and species that could not be detectable by other methods. The average oxidation state of marine particulate iron determined by chemical-species mapping is comparable to that determined by standard bulk X-ray Absorption Near Edge Structure spectroscopy. Using chemical-species mapping, we find that up to 43% of particulate Fe in the Northwest Pacific at the depth of the adjacent active continental margin is in the Fe(II) state, with the balance Fe(III). In contrast, particulate iron in the eastern tropical North Atlantic, which receives the highest dust deposition on Earth and is adjacent to a passive margin, is dominated by weathered and oxidized Fe compounds, with Fe(III) contributing 90% of total iron. The balance is composed primarily of Fe(II)-containing species, but we detected individual pyrite particles in some samples within an oxygen minimum zone in the upper thermocline. Several lines of evidence point to the adjacent Mauritanian continental shelf as the source of pyrite to the water column. The speciation of suspended marine particulate iron reflects the mineralogy of iron from the adjacent continental margins. Since the solubility of particulate iron has been shown to be a function of its speciation, this may have implications for the bioavailability of particulate iron adjacent to passive compared to active continental margins.

  1. The mechanisms and meaning of the mismatch negativity.

    Fishman, Yonatan I

    2014-07-01

    The mismatch negativity (MMN) is a pre-attentive auditory event-related potential (ERP) component that is elicited by a change in a repetitive acoustic pattern. It is obtained by subtracting responses evoked by frequent 'standard' sounds from responses evoked by infrequent 'deviant' sounds that differ from the standards along some acoustic dimension, e.g., frequency, intensity, or duration, or abstract feature. The MMN has been attributed to neural generators within the temporal and frontal lobes. The mechanisms and meaning of the MMN continue to be debated. Two dominant explanations for the MMN have been proposed. According to the "neural adaptation" hypothesis, repeated presentation of the standards results in adapted (i.e., attenuated) responses of feature-selective neurons in auditory cortex. Rare deviant sounds activate neurons that are less adapted than those stimulated by the frequent standard sounds, and thus elicit a larger 'obligatory' response, which yields the MMN following the subtraction procedure. In contrast, according to the "sensory memory" hypothesis, the MMN is a 'novel' (non-obligatory) ERP component that reflects a deviation between properties of an incoming sound and those of a neural 'memory trace' established by the preceding standard sounds. Here, we provide a selective review of studies which are relevant to the controversy between proponents of these two interpretations of the MMN. We also present preliminary neurophysiological data from monkey auditory cortex with potential implications for the debate. We conclude that the mechanisms and meaning of the MMN are still unresolved and offer remarks on how to make progress on these important issues. PMID:24276221

  2. Approaches to diagnose DNA mismatch repair gene defects in cancer.

    Peña-Diaz, Javier; Rasmussen, Lene Juel

    2016-02-01

    The DNA repair pathway mismatch repair (MMR) is responsible for the recognition and correction of DNA biosynthetic errors caused by inaccurate nucleotide incorporation during replication. Faulty MMR leads to failure to address the mispairs or insertion deletion loops (IDLs) left behind by the replicative polymerases and results in increased mutation load at the genome. The realization that defective MMR leads to a hypermutation phenotype and increased risk of tumorigenesis highlights the relevance of this pathway for human disease. The association of MMR defects with increased risk of cancer development was first observed in colorectal cancer patients that carried inactivating germline mutations in MMR genes and the disease was named as hereditary non-polyposis colorectal cancer (HNPCC). Currently, a growing list of cancers is found to be MMR defective and HNPCC has been renamed Lynch syndrome (LS) partly to include the associated risk of developing extra-colonic cancers. In addition, a number of non-hereditary, mostly epigenetic, alterations of MMR genes have been described in sporadic tumors. Besides conferring a strong cancer predisposition, genetic or epigenetic inactivation of MMR genes also renders cells resistant to some chemotherapeutic agents. Therefore, diagnosis of MMR deficiency has important implications for the management of the patients, the surveillance of their relatives in the case of LS and for the choice of treatment. Some of the alterations found in MMR genes have already been well defined and their pathogenicity assessed. Despite this substantial wealth of knowledge, the effects of a large number of alterations remain uncharacterized (variants of uncertain significance, VUSs). The advent of personalized genomics is likely to increase the list of VUSs found in MMR genes and anticipates the need of diagnostic tools for rapid assessment of their pathogenicity. This review describes current tools and future strategies for addressing the relevance

  3. Mismatch Negativity Latency and Cognitive Function in Schizophrenia

    Kärgel, Christian; Sartory, Gudrun; Kariofillis, Daniela; Wiltfang, Jens; Müller, Bernhard W.

    2014-01-01

    Background The Mismatch Negativity (MMN) is an event-related potential (ERP) sensitive to early auditory deviance detection and has been shown to be reduced in schizophrenia patients. Moreover, MMN amplitude reduction to duration deviant tones was found to be related to functional outcomes particularly, to neuropsychological (working memory and verbal domains) and psychosocial measures. While MMN amplitude is thought to be correlated with deficits of early sensory processing, the functional significance of MMN latency remains unclear so far. The present study focused on the investigation of MMN in relation to neuropsychological function in schizophrenia. Method Forty schizophrenia patients and 16 healthy controls underwent a passive oddball paradigm (2400 binaural tones; 88% standards [1 kHz, 80 db, 80 ms], 11% frequency deviants [1.2 kHz], 11% duration deviants [40 ms]) and a neuropsychological test-battery. Patients were assessed with regard to clinical symptoms. Results Compared to healthy controls schizophrenia patients showed diminished MMN amplitude and shorter MMN latency to both deviants as well as an impaired neuropsychological test performance. Severity of positive symptoms was related to decreased MMN amplitude to duration deviants. Furthermore, enhanced verbal memory performance was associated with prolonged MMN latency to frequency deviants in patients. Conclusion The present study corroborates previous results of a diminished MMN amplitude and its association with positive symptoms in schizophrenia patients. Both, the findings of a shorter latency to duration and frequency deviants and the relationship of the latter with verbal memory in patients, emphasize the relevance of the temporal aspect of early auditory discrimination processing in schizophrenia. PMID:24740391

  4. Ventilation/perfusion mismatch during lung aeration at birth.

    Lang, Justin A R; Pearson, James T; te Pas, Arjan B; Wallace, Megan J; Siew, Melissa L; Kitchen, Marcus J; Fouras, Andreas; Lewis, Robert A; Wheeler, Kevin I; Polglase, Graeme R; Shirai, Mikiyasu; Sonobe, Takashi; Hooper, Stuart B

    2014-09-01

    At birth, the transition to newborn life is triggered by lung aeration, which stimulates a large increase in pulmonary blood flow (PBF). Current theories predict that the increase in PBF is spatially related to ventilated lung regions as they aerate after birth. Using simultaneous phase-contrast X-ray imaging and angiography we investigated the spatial relationships between lung aeration and the increase in PBF after birth. Six near-term (30-day gestation) rabbits were delivered by caesarean section, intubated and an intravenous catheter inserted, before they were positioned for X-ray imaging. During imaging, iodine was injected before ventilation onset, after ventilation of the right lung only, and after ventilation of both lungs. Unilateral ventilation increased iodine levels entering both left and right pulmonary arteries (PAs) and significantly increased heart rate, iodine ejection per beat, diameters of both left and right PAs, and number of visible vessels in both lungs. Within the 6th intercostal space, the mean gray level (relative measure of iodine level) increased from 68.3 ± 11.6 and 70.3 ± 7.5%·s to 136.3 ± 22.6 and 136.3 ± 23.7%·s in the left and right PAs, respectively. No differences were observed between vessels in the left and right lungs, despite the left lung not initially being ventilated. The increase in PBF at birth is not spatially related to lung aeration allowing a large ventilation/perfusion mismatch, or pulmonary shunting, to occur in the partially aerated lung at birth. PMID:24994883

  5. Unusual Mismatch Repair Immunohistochemical Patterns in Endometrial Carcinoma.

    Watkins, Jaclyn C; Nucci, Marisa R; Ritterhouse, Lauren L; Howitt, Brooke E; Sholl, Lynette M

    2016-07-01

    Universal screening for Lynch syndrome through mismatch repair (MMR) immunohistochemistry (IHC) on tumor samples has brought to light several heterogenous MMR staining patterns. At our institution, a prospective study of universal Lynch syndrome screening using MMR IHC on 125 endometrial cancers (EC) led to the identification of subclonal loss of MMR protein expression within the tumor (n=9). We also interrogated the MMR staining patterns in MMR-deficient EC with concurrent endometrial intraepithelial neoplasia (EIN; n=14) and all mixed-type ECs (n=14) to look for concordant or discordant profiles between the various components. MLH1 promoter methylation and microsatellite instability testing was performed on discordant subclones. Abrupt and complete subclonal loss of MMR expression was identified in 9 cases (7.2%; 7 subclonal MLH1/PMS2 loss, 1 subclonal loss of MLH1 and complete loss of PMS2, and 1 subclonal MSH6 loss). All subclonal MLH1 losses were associated with epigenetic silencing. In cases with concomitant EIN (n=14), 7 cases showed concordant MMR IHC between EC and EIN, and 4 cases showed MMR protein loss confined to the EC. The remaining 3 cases demonstrated subclonal staining in the EIN. In mixed tumors (n=14), subclonal or total MMR IHC deficiency was confined to endometrioid components. In summary, discrete subclonal loss of MMR protein expression occurs in up to 7.2% of EC and, in our experience, only in endometrioid components. Importantly, subclonal MLH1 MMR defects appear to be a biological phenomenon that can be explained by methylation and somatic events, without evidence of underlying germline alterations. PMID:27186853

  6. Processing of unattended facial emotions: a visual mismatch negativity study.

    Stefanics, Gábor; Csukly, Gábor; Komlósi, Sarolta; Czobor, Pál; Czigler, István

    2012-02-01

    Facial emotions express our internal states and are fundamental in social interactions. Here we explore whether the repetition of unattended facial emotions builds up a predictive representation of frequently encountered emotions in the visual system. Participants (n=24) were presented peripherally with facial stimuli expressing emotions while they performed a visual detection task presented in the center of the visual field. Facial stimuli consisted of four faces of different identity, but expressed the same emotion (happy or fearful). Facial stimuli were presented in blocks of oddball sequence (standard emotion: p=0.9, deviant emotion: p=0.1). Event-related potentials (ERPs) to the same emotions were compared when the emotions were deviant and standard, respectively. We found visual mismatch negativity (vMMN) responses to unattended deviant emotions in the 170-360 ms post-stimulus range over bilateral occipito-temporal sites. Our results demonstrate that information about the emotional content of unattended faces presented at the periphery of the visual field is rapidly processed and stored in a predictive memory representation by the visual system. We also found evidence that differential processing of deviant fearful faces starts already at 70-120 ms after stimulus onset. This finding shows a 'negativity bias' under unattended conditions. Differential processing of fearful deviants were more pronounced in the right hemisphere in the 195-275 ms and 360-390 ms intervals, whereas processing of happy deviants evoked larger differential response in the left hemisphere in the 360-390 ms range, indicating differential hemispheric specialization for automatic processing of positive and negative affect. PMID:22037000

  7. Effect of aripiprazole on mismatch negativity (MMN in schizophrenia.

    Zhenhe Zhou

    Full Text Available BACKGROUND: Cognitive deficits are considered core symptoms of the schizophrenia. Cognitive function has been found to be a better predictor of functional outcome than symptom levels. Changed mismatch negativity (MMN reflects abnormalities of early auditory processing in schizophrenia. Up to now, no studies for the effects of aripiprazole on MMN in schizophrenia have been reported. METHODOLOGY/PRINCIPAL FINDINGS: Subjects included 26 patients with schizophrenia, and 26 controls. Psychopathology was rated in patients with the Positive and Negative Syndrome Scale (PANSS at baseline, after 4- and 8-week treatments with aripiprazole. Auditory stimuli for ERP consisted of 100 millisecond/1000 Hz standards, intermixed with 100 millisecond/1500 Hz frequency deviants and 250 millisecond/1000 Hz duration deviants. EEG was recorded at Fz. BESA 5.1.8 was used to perform data analysis. MMN waveforms were obtained by subtracting waveforms elicited by standards from waveforms elicited by frequency- or duration-deviant stimuli. Aripiprazole decreased all PANSS. Patients showed smaller mean amplitudes of frequency and duration MMN at baseline than did controls. A repeated measure ANOVA with sessions (i.e., baseline, 4- and 8-week treatments and MMN type (frequency vs. duration as within-subject factors revealed no significant MMN type or MMN type × session main effect for MMN amplitudes. Session main effect was significant. LSD tests demonstrated significant differences between MMN amplitudes at 8 weeks and those at both baseline and 4 weeks. There was significant negative correlation between changes in amplitudes of frequency and duration MMN and changes in PANSS total scores at baseline and follow-up periods. CONCLUSIONS: Aripiprazole improved the amplitudes of MMN. MMN offers objective evidence that treatment with the aripiprazole may ameliorate preattentive deficits in schizophrenia.

  8. Study on relationship between historical volcanic eruptions and historical strong earthquakes in China and its adjacent regions

    1999-01-01

    This thesis lists and describes 6 pairs of tectonic events, i.e., historical volcanic eruptions associated with historical strong earthquakes, based on the analysis for the records of historical volcanic eruptions and historical strong earthquakes in China and its adjacent region since the first record. And discusses the relationship between historical eruptions and strong earthquakes by means of analyzing the characteristics of tectonic events themselves, plate movement, regional seismicity, and regional stress environment in China and its adjacent region.

  9. Perceived match or mismatch on the Gottman conflict styles: associations with relationship outcome variables.

    Busby, Dean M; Holman, Thomas B

    2009-12-01

    Gottman has proposed that there are 3 functional styles of conflict management in couple relationships, labeled Avoidant, Validating, and Volatile, and 1 dysfunctional style, labeled Hostile. Using a sample of 1,983 couples in a committed relationship, we test the association of perceived matches or mismatches on these conflict styles with relationship outcome variables. The results indicate that 32% of the participants perceive there is a mismatch with their conflict style and that of their partner. The Volatile-Avoidant mismatch was particularly problematic and was associated with more stonewalling, relationship problems, and lower levels of relationship satisfaction and stability than the Validating matched style and than other mismatched styles. The most problematic style was the Hostile style. Contrary to existing assumptions by Gottman, the 3 matched functional styles were not equivalent, as the Validating Style was associated with substantially better results on relationship outcome measures than the Volatile and Avoidant styles. PMID:19930437

  10. Role of Energy-Level Mismatches in a Multi-Pathway Complex of Photosynthesis

    Lim, James; Lee, Changhyoup; Yoo, Seokwon; Jeong, Hyunseok; Lee, Jinhyoung

    2013-01-01

    Considering a multi-pathway structure in a light-harvesting complex of photosynthesis, we investigate the role of energy-level mismatches between antenna molecules in transferring the absorbed energy to a reaction center. We find a condition in which the antenna molecules faithfully play their roles: Their effective absorption ratios are larger than those of the receiver molecule directly coupled to the reaction center. In the absence of energy-level mismatches and dephasing noise, there arises quantum destructive interference between multiple paths that restricts the energy transfer. On the other hand, the destructive interference diminishes as asymmetrically biasing the energy-level mismatches and/or introducing quantum noise of dephasing for the antenna molecules, so that the transfer efficiency is greatly enhanced to near unity. Remarkably, the near-unity efficiency can be achieved at a wide range of asymmetric energy-level mismatches. Temporal characteristics are also optimized at the energy-level mismat...

  11. Influences of Device and Circuit Mismatches on Paralleling Silicon Carbide MOSFETs

    Li, Helong; Munk-Nielsen, Stig; Wang, Xiongfei;

    2016-01-01

    This paper addresses the influences of device and circuit mismatches on paralleling the Silicon Carbide (SiC) MOSFETs. Comprehensive theoretical analysis and experimental validation from paralleled discrete devices to paralleled dies in multichip power modules are first presented. Then, the...... influence of circuit mismatch on paralleling SiC MOSFETs is investigated and experimentally evaluated for the first time. It is found that the mismatch of the switching loop stray inductance can also lead to on-state current unbalance with inductive output current, in addition to the on-state resistance of...... the device. It further reveals that circuit mismatches and a current coupling among the paralleled dies exist in a SiC MOSFET multichip power module, which is critical for the transient current distribution in the power module. Thus, a power module layout with an auxiliary source connection is...

  12. Negative effects of a disulfide bond mismatch in anti-rabies G protein single-chain antibody variable fragment FV57.

    Duan, Ye; Gu, Tiejun; Zhang, Xizhen; Jiang, Chunlai; Yuan, Ruosen; Li, Zhuang; Wang, Dandan; Chen, Xiaoxu; Wu, Chunlai; Chen, Yan; Wu, Yongge; Kong, Wei

    2014-06-01

    Rabies virus (RV) causes a fatal infectious disease requiring efficient post-exposure prophylaxis (PEP), which includes a rabies vaccine and rabies immunoglobulin (RIG). The single-chain antibody variable fragment (scFv), a small engineered antibody fragment derived from an antibody variable heavy chain and light chain, has the potential to replace the current application of RIG. In previous studies, we constructed and evaluated an anti-rabies virus G protein scFv (FV57) based on the monoclonal antibody CR57. Of the five cysteines in FV57, four are linked in intra-chain disulfide bonds (Cys-VH28/Cys-VH98 and Cys-VL16/Cys-VL84), and one is free (Cys-VL85). However, the thiol in Cys-VL85 neighboring Cys-VL84 in the CDR3 of the light chain is likely to mismatch with the thiol in Cys-VL16 during the renaturing process. In order to study effects of the mismatched disulfide bond, Cys-VL85 and Cys-VL84 of FV57 were mutated to serine to construct mutants FV57(VL85S) and FV57(VL84S). Furthermore, the disulfide bonds in the light chain of FV57, FV57(VL85S) and FV57(VL84S) were deleted by mutating Cys-VL16 to serine. All mutants were prepared and evaluated along with the original FV57. The results indicated that the mismatched disulfide bond of FV57 linking the light chain FR1 and CDR3 would confer deleterious negative effects on its activity against RV, likely due to spatial hindrance in the light chain CDR3. Moreover, avoidance of the disulfide bond mismatch provided an additional 30% protective efficacy against RV infection in the mouse RV challenge model. Thus, modifications of FV57 to eliminate the disulfide bond mismatch may provide a candidate therapeutic agent for effective PEP against rabies. PMID:24598312

  13. Mismatch repair at stop codons is directed independent of GATC methylation on the Escherichia coli chromosome.

    Sneppen, Kim; Semsey, Szabolcs

    2014-01-01

    The mismatch repair system (MMR) corrects replication errors that escape proofreading. Previous studies on extrachromosomal DNA in Escherichia coli suggested that MMR uses hemimethylated GATC sites to identify the newly synthesized strand. In this work we asked how the distance of GATC sites and their methylation status affect the occurrence of single base substitutions on the E. coli chromosome. As a reporter system we used a lacZ gene containing an early TAA stop codon. We found that occurrence of point mutations at this stop codon is unaffected by GATC sites located more than 115 base pairs away. However, a GATC site located about 50 base pairs away resulted in a decreased mutation rate. This effect was independent of Dam methylation. The reversion rate of the stop codon increased only slightly in dam mutants compared to mutL and mutS mutants. We suggest that unlike on extrachromosomal DNA, GATC methylation is not the only strand discrimination signal for MMR on the E. coli chromosome. PMID:25475788

  14. Association of common variants in mismatch repair genes and breast cancer susceptibility: a multigene study

    Pina Julieta

    2009-09-01

    Full Text Available Abstract Background MMR is responsible for the repair of base-base mismatches and insertion/deletion loops. Besides this, MMR is also associated with an anti-recombination function, suppressing homologous recombination. Losses of heterozygosity and/or microsatellite instability have been detected in a large number of skin samples from breast cancer patients, suggesting a potential role of MMR in breast cancer susceptibility. Methods We carried out a hospital-based case-control study in a Caucasian Portuguese population (287 cases and 547 controls to estimate the susceptibility to non-familial breast cancer associated with some polymorphisms in mismatch repair genes (MSH3, MSH4, MSH6, MLH1, MLH3, PMS1 and MUTYH. Results Using unconditional logistic regression we found that MLH3 (L844P, G>A polymorphism GA (Leu/Pro and AA (Pro/Pro genotypes were associated with a decreased risk: OR = 0.65 (0.45-0.95 (p = 0.03 and OR = 0.62 (0.41-0.94 (p = 0.03, respectively. Analysis of two-way SNP interaction effects on breast cancer revealed two potential associations to breast cancer susceptibility: MSH3 Ala1045Thr/MSH6 Gly39Glu - AA/TC [OR = 0.43 (0.21-0.83, p = 0.01] associated with a decreased risk; and MSH4 Ala97Thr/MLH3 Leu844Pro - AG/AA [OR = 2.35 (1.23-4.49, p = 0.01], GG/AA [OR = 2.11 (1.12-3,98, p = 0.02], and GG/AG [adjusted OR = 1.88 (1.12-3.15, p = 0.02] all associated with an increased risk for breast cancer. Conclusion It is possible that some of these common variants in MMR genes contribute significantly to breast cancer susceptibility. However, further studies with a large sample size will be needed to support our results.

  15. Do anticodons of misacylated tRNAs preferentially mismatch codons coding for the misloaded amino acid?

    Seligmann Hervé

    2010-05-01

    Full Text Available Abstract Background Accurate amino acid insertion during peptide elongation requires tRNAs loaded by cognate amino acids and that anticodons match codons. However, tRNA misloading does not necessarily cause misinsertions: misinsertion is avoided when anticodons mismatch codons coding for misloaded amino acids. Presentation of the hypothesis Occasional compensation of misacylation by codon-anticodon mismatch necessarily occurs. Putatively, occasional error compensation may be enhanced beyond the random combination of independent errors in tRNA loading and codon-anticodon interactions: tRNA misacylation might alter potentials for codon-anticodon mismatches, perhaps specifically increasing potentials for mismatching those codons coding for the misacylated non-cognate amino acid. This hypothetical phenomenon is called 'error coordination', in distinction from 'error compensation' that assumes independence between misacylation and mismatch. Testing the hypothesis Eventually, the hypothesis should be tested for each combination of amino acid misacylation and codon-anticodon mismatch, by comparing stabilities or frequencies of mismatched codon-anticodon duplexes formed by tRNAs loaded by their cognate amino acid with stabilities formed by that tRNA when misloaded with the amino acid coded by the mismatched codon. Competitive mismatching experiments between misloaded and correctly loaded tRNAs could also be useful, yet more sophisticated experiments. Implications of the hypothesis Detecting error coordination implies estimating error compensation, which also promotes protein synthesis accuracy. Hence even in the absence of evidence for error coordination, experiments would yield very useful insights into misacylation and mismatch processes. In case experiments consider post-transcriptional RNA modifications (especially at wobble positions, results on codon-anticodon mismatches would enable significant improvements and sophistications of secondary

  16. Increasing efficiency of cold rolling of corrosion resistant steel at mismatching roll velocities

    On the example of 12Kh18N10T steel rolling it is shown that 7-10% mismatching roll velocities raise the rolling efficiency, increase productivity and technological abilities of four-high mills at production of cold-rolled sheets. Scheme of the system of roll velocities mismatching control at asymmetrical rolling is presented. The comparative conditions of symmetrical and asymmetrical rolling of 0.8 x 1030 mm sheets from 12Kh18N10T steel are given

  17. Cernunnos/XLF promotes the ligation of mismatched and noncohesive DNA ends

    Tsai, Chun J.; Kim, Sunny A.; Chu, Gilbert

    2007-01-01

    Nonhomologous end-joining (NHEJ) repairs DNA double-strand breaks created by ionizing radiation or V(D)J recombination of the immunoglobulin genes. The breaks often leave mismatched or nonligatable ends, and NHEJ must repair the breaks with high efficiency and minimal nucleotide loss. Here, the NHEJ proteins Ku, DNA-dependent protein kinase catalytic subunit, XRCC4/Ligase IV, and Cernunnos/XRCC4-like factor joined mismatched and noncohesive DNA ends in the absence of processing factors. Depen...

  18. “Job Accessibility, Employment and Job-Education Mismatch in the Metropolitan Area of Barcelona”

    Antonio Di Paolo; Anna Matas; Josep Lluís Raymond

    2014-01-01

    This paper analyses the effect of job accessibility by public and private transport on labour market outcomes in the metropolitan area of Barcelona. Beyond employment, we consider the effect of job accessibility on job-education mismatch, which represents a relevant aspect of job quality. We adopt a recursive system of equations that models car availability, employment and mismatch. Public transport accessibility appears as an exogenous variable in the three equations. Even though it may refl...

  19. Education, educational mismatch, and wage inequality: evidence for different european countries

    Santos, Marcelo Serra

    2012-01-01

    In this dissertation, we study the relationship of mismatch between workers’ education and labor market requirements throughout different European countries. We found evidence in several countries that overeducated people tend to have a wage penalty and undereducated people tend to have a wage premium. This evidence contradicts the few existing evidence on the issue. However, despite the typical effects of education, tenure, experience, and gender in wages, the effects of mismatch between edu...

  20. Are manufacturing I-V Mismatch and Reverse Currents Key Factors in Large Photovoltaic Arrays?

    Spertino, Filippo; Sumaili Akilimali, Jean

    2009-01-01

    In this paper, two factors typical of large photovoltaic (PV) arrays are investigated: one is the current-voltage (I-V) mismatch consequent to the production tolerance; the other is the impact of reverse currents in different operating conditions. Concerning the manufacturing I-V mismatch, the parameters of the equivalent circuit of the solar cell are computed for several PV modules from flash reports provided by the manufacturers. The corresponding I-V characteristic of every module is used ...

  1. The incidence and etiology of the ventilation/perfusion reverse mismatch defect

    Kr-81m ventilation and Tc-99m perfusion images of 392 patients were examined retrospectively for the incidence and etiology of the reverse mismatch defect, which is characterized by a region of lung where the perfusion defect exceeds the ventilation defect. Forty-six patients (11.7%) showed such defects. The most frequent causes were pneumonia (15%), atelactasis (15%), pleural effusions (15%), chronic obstructive airway disease (24%), and bronchial obstruction (31%). The significance of the reverse mismatch defect is discussed

  2. Robust Steering Vector Mismatch Techniques for Reduced Rank Adaptive Array Signal Processing

    Nguyen, Hien

    2002-01-01

    The research presented in this dissertation is on the development of advanced reduced rank adaptive signal processing for airborne radar space-time adaptive processing (STAP) and steering vector mismatch robustness. This is an important area of research in the field of airborne radar signal processing since practical STAP algorithms should be robust against various kinds of mismatch errors. The clutter return in an airborne radar has widely spread Doppler frequencies; therefore STAP, a two-di...

  3. Wage effects of job-worker mismatches: Heterogeneous skills or institutional effects?

    Allen J.P.; Velden R.K.W. van der; Badillo-Amador L.

    2013-01-01

    The strong wage effects related to mismatches between a workers education and that required in the job are usually attributed to assignment theory. This theory asserts that productivity and wages depend on the education-job match, which determines the utilization of skills. However, recent research shows that educational mismatches are only weakly related to skill utilization, which in any case fails to account for the bulk of the wage effects. Two alternative theories have been put forward t...

  4. Mismatch or allostatic load? Timing of life adversity differentially shapes gray matter volume and anxious temperament

    Kuhn, Manuel; Scharfenort, Robert; Schümann, Dirk; Schiele, Miriam A; Münsterkötter, Anna L.; Deckert, Jürgen; Domschke, Katharina; Haaker, Jan; Kalisch, Raffael; Pauli, Paul; Reif, Andreas; Romanos, Marcel; Zwanzger, Peter; Lonsdorf, Tina B.

    2015-01-01

    Traditionally, adversity was defined as the accumulation of environmental events (allostatic load). Recently however, a mismatch between the early and the later (adult) environment (mismatch) has been hypothesized to be critical for disease development, a hypothesis that has not yet been tested explicitly in humans. We explored the impact of timing of life adversity (childhood and past year) on anxiety and depression levels (N = 833) and brain morphology (N = 129). Both remote (childhood) and...

  5. Patient-prosthesis mismatch and reduction in left ventricular mass after aortic valve replacement

    Kandler, Kristian; Møller, Christian H; Hassager, Christian;

    2013-01-01

    The presence of patient-prosthesis mismatch (PPM) after aortic valve replacement may influence patient survival. We examined the relationship between PPM and changes in left ventricular mass index at 3 months follow-up and also overall survival.......The presence of patient-prosthesis mismatch (PPM) after aortic valve replacement may influence patient survival. We examined the relationship between PPM and changes in left ventricular mass index at 3 months follow-up and also overall survival....

  6. Impact of prosthesis-patient mismatch on survival after mitral valve replacement: a systematic review

    ZHANG Jian-feng; WU Yi-cheng; SHEN Wei-feng; KONG Ye

    2013-01-01

    Objective To determine whether the prosthesis-patient mismatch has a deleterious impact on survival after mitral valve replacement.Data sources A comprehensive literature search of PubMed,Embase,and ScienceDirect was carried out.References and cited papers of relevant articles were also checked.Study selection All articles published after January 1980 was initially considered.Non-English and non-human studies,case reports,and reviews were excluded from the initial search.References and cited papers of relevant articles were also checked.Results A total of 8 retrospective cohort studies were identified for this review.The overall incidence of prosthesis-patient mismatch (<1.3 to <1.2 cm2/m2) after mitral valve replacement ranged from 3.7% to 85.9% (moderate prosthesis-patient mismatch (0.9 to 1.2 cm2/m2) in 37.4% to 69.5%,severe prosthesis-patient mismatch (<0.9 cm2/m2) in 8.7% to 16.4%).Four studies demonstrated an association of prosthesis-patient mismatch with reduced long-term survival,but the other four studies found no significant deleterious impact of prosthesis-patient mismatch after mitral valve replacement.No definite conclusion could be derived from these conflicting results.Conclusions Current evidence is insufficient to derive a definite conclusion whether mitral prosthesis-patient mismatch affects long-term survival because of the biases and confounding factors that interfere with late clinical outcomes.Good-quality prospective studies are warranted to evaluate the impact of mitral prosthesis-patient mismatch after mitral valve replacement in the future.

  7. Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork

    Klocko, Andrew D.; Schroeder, Jeremy W.; Walsh, Brian W.; Lenhart, Justin S.; Evans, Margery L.; Simmons, Lyle A.

    2011-01-01

    Mismatch repair (MMR) corrects DNA polymerase errors occurring during genome replication. MMR is critical for genome maintenance, and its loss increases mutation rates several hundredfold. Recent work has shown that the interaction between the mismatch recognition protein MutS and the replication processivity clamp is important for MMR in Bacillus subtilis. To further understand how MMR is coupled to DNA replication, we examined the subcellular localization of MMR and DNA replication proteins...

  8. Detecting mismatches of bird migration stopover and tree phenology in response to changing climate

    Kellermann, Jherime L.; Van Riper, Charles, III

    2015-01-01

    Migratory birds exploit seasonal variation in resources across latitudes, timing migration to coincide with the phenology of food at stopover sites. Differential responses to climate in phenology across trophic levels can result in phenological mismatch; however, detecting mismatch is sensitive to methodology. We examined patterns of migrant abundance and tree flowering, phenological mismatch, and the influence of climate during spring migration from 2009 to 2011 across five habitat types of the Madrean Sky Islands in southeastern Arizona, USA. We used two metrics to assess phenological mismatch: synchrony and overlap. We also examined whether phenological overlap declined with increasing difference in mean event date of phenophases. Migrant abundance and tree flowering generally increased with minimum spring temperature but depended on annual climate by habitat interactions. Migrant abundance was lowest and flowering was highest under cold, snowy conditions in high elevation montane conifer habitat while bird abundance was greatest and flowering was lowest in low elevation riparian habitat under the driest conditions. Phenological synchrony and overlap were unique and complementary metrics and should both be used when assessing mismatch. Overlap declined due to asynchronous phenologies but also due to reduced migrant abundance or flowering when synchrony was actually maintained. Overlap declined with increasing difference in event date and this trend was strongest in riparian areas. Montane habitat specialists may be at greatest risk of mismatch while riparian habitat could provide refugia during dry years for phenotypically plastic species. Interannual climate patterns that we observed match climate change projections for the arid southwest, altering stopover habitat condition.

  9. Detection of cognitive impairment in patients with obstructive sleep apnea hypopnea syndrome using mismatch negativity

    Xiaohui Wen; Ningyu Wang; Jinfeng Liu; Zhanfeng Yan; Zhonghai Xin

    2012-01-01

    In this experiment, 97 patients with obstructive sleep apnea hypopnea syndrome were divided into three groups (mild, moderate, severe) according to minimum oxygen saturation, and 35 healthy subjects were examined as controls. Cognitive function was determined using the mismatch negativity paradigm and the Montreal Cognitive Assessment. The results revealed that as the disease worsened, the mismatch negativity latency was gradually extended, and the amplitude gradually declined in patients with obstructive sleep apnea hypopnea syndrome. Importantly, mismatch negativity latency in severe patients with a persistent time of minimum oxygen saturation 60 seconds. Correlation analysis revealed a negative correlation between minimum oxygen saturation latency and Montreal Cognitive Assessment scores. These findings indicate that intermittent night-time hypoxemia affects mismatch negativity waveforms and Montreal Cognitive Assessment scores. As indicators for detecting the cognitive functional status of obstructive sleep apnea hypopnea syndrome patients, the sensitivity of mismatch negativity is 82.93%, the specificity is 73.33%, the accuracy rate is 81.52%, the positive predictive value is 85.00%, the negative predictive value is 70.21%, the positive likelihood ratio is 3, and the negative likelihood ratio is 0.23. These results indicate that mismatch negativity can be used as an effective tool for diagnosis of cognitive dysfunction in obstructive sleep apnea hypopnea syndrome patients.

  10. Experimental measurement of emittance growth in mismatched space-charge-dominated beams

    Using the Single Beam Transport Experiment (SBTE) at LBL, we have measured the emittance of a well-matched 4.6-mA beam of 122-keV Cs+ to be conserved from injection into through exit from an 80-lens segment of the AG focussing channel. We then mismatched the beam into the same channel such that the maximum (minimum) radius of the beam at the midplane between lenses was about 1.5 (0.5) times the former value. We caused mismatches in the envelope of the beam in both transverse dimensions (labeled a and b) in modes both symmetric (δa = δb) and antisymmetric (δa = -δb). We found the mismatch amplitude to decay during the beam transit through the channel for both modes of mismatch, although more so for the antisymmetric mode. We also found the emittance of the symmetrically mismatched beam to be the same as for the matched beam, while the emittance of the antisymmetrically mismatched beam grew by as much as a factor of four over that for the matched beam

  11. Phenological mismatch and ontogenetic diet shifts interactively affect offspring condition in a passerine.

    Samplonius, Jelmer M; Kappers, Elena F; Brands, Stef; Both, Christiaan

    2016-09-01

    Climate change may cause phenological asynchrony between trophic levels, which can lead to mismatched reproduction in animals. Although indirect effects of mismatch on fitness are well described, direct effects on parental prey choice are not. Moreover, direct effects of prey variation on offspring condition throughout their early development are understudied. Here, we used camera trap data collected over 2 years to study the effects of trophic mismatch and nestling age on prey choice in pied flycatchers (Ficedula hypoleuca). Furthermore, we studied the effect of mismatch and variation in nestling diet on offspring condition. Both experimentally induced and natural mismatches with the caterpillar peak negatively affected absolute and relative numbers of caterpillars and offspring condition (mass, tarsus and wing length) and positively affected absolute and relative numbers of flying insects in the nestling diet. Feeding more flying insects was negatively correlated with nestling day 12 mass. Both descriptive and experimental data showed preferential feeding of spiders when nestlings were spiders during this phase was positively correlated with tarsus growth. These results highlight the need for a more inclusive framework to study phenological mismatch in nature. The general focus on only one prey type, the rarity of studies that measure environmental abundance of prey, and the lack of timing experiments in dietary studies currently hamper understanding of the actual trophic interactions that affect fitness under climate change. PMID:27263989

  12. HLA mismatching as a strategy to reduce relapse after alternative donor transplantation.

    Fleischhauer, Katharina; Beelen, Dietrich W

    2016-04-01

    Human leukocyte antigen (HLA) mismatches are targets of alloreactive T cells, mediators of graft-versus-leukemia (GvL) and graft-versus-host disease (GvHD) after alternative donor transplantation. Exploitation of HLA mismatching in order to reduce relapse is hampered by necessary interventions aimed at controlling GvHD on the one hand, and by the possibility of immune escape through selective loss of mismatched HLA in relapsing leukemia on the other. Retrospective studies reporting the impact of HLA mismatches on post-transplant relapse need to be interpreted with caution, due to many confounding factors, including disease and use of T-cell depletion, and to be constantly updated to the rapidly changing clinical protocols. Current evidence suggests similar relapse rates for 8/8, 7/8 HLA-matched unrelated, T-cell-replete haploidentical and umbilical cord blood transplantation; however, investigations of locus-specific effects are still scarce in the latter two settings. In unrelated transplantation, a specific role for mismatches at HLA-C and HLA-DPB1, and therein of permissive mismatches defined on the basis of T-cell alloreactivity and/or expression levels, in reducing relapse has been demonstrated in independent studies. This observation suggests new approaches to utilize HLA matching in unrelated donor searches, and the need for further research in the field. PMID:27000727

  13. Small Mismatches in Fatty Acyl Tail Lengths Can Effect Non Steroidal Anti-Inflammatory Drug Induced Membrane Fusion.

    Majumdar, Anupa; Sarkar, Munna

    2016-06-01

    Biological membranes are made up of a variety of lipids with diverse physicochemical properties. The lipid composition modulates different lipidic parameters, such as hydration, dynamics, lipid packing, curvature strain, etc. Changes in these parameters affect various membrane-mediated processes, such as membrane fusion which is an integral step in many biological processes. Packing defects, which originate either from mismatch in the headgroup region or in the hydrophobic acyl tail region, play a major role in modulating membrane dynamics. In this study, we demonstrate how even a small mismatch in the fatty acyl chain length, achieved by incorporation of low concentrations (up to 30 mol %) of dipalmitoylphosphatidylcholine (DPPC) into dimyristoylphosphatidylcholine (DMPC) small unilamellar vesicles (SUVs), alters several lipidic parameters like packing, dynamics, and headgroup hydration. This in turn affects non steroidal anti-inflammatory drug (NSAID) induced membrane fusion. Dynamic light scattering, differential scanning calorimetry, second-derivative absorption spectrophotometry, and steady-state and time-resolved fluorescence have been used to elucidate the effect of small mismatch in the tails in DMPC/DPPC mixed vesicles and how it modulates membrane fusion induced by the oxicam NSAIDs, meloxicam (Mx), piroxicam (Px), and tenoxicam (Tx). Fusion kinetics was monitored using fluorescence based fusion assays. At low DPPC concentration of 10 mol %, additional fluidization promotes lipid mixing to some extent for Mx, but at higher mol % of DPPC, subsequent increase in rigidity of membrane interior along with increase in headgroup hydration, synergistically inhibits fusion to various extents for the three different drugs, Mx, Px, and Tx. PMID:27153337

  14. Wall shear stress in intracranial aneurysms and adjacent arteries

    Fuyu Wang; Bainan Xu; Zhenghui Sun; Chen Wu; Xiaojun Zhang

    2013-01-01

    Hemodynamic parameters play an important role in aneurysm formation and growth. However, it is difficult to directly observe a rapidly growing de novo aneurysm in a patient. To investigate possible associations between hemodynamic parameters and the formation and growth of intracranial aneurysms, the present study constructed a computational model of a case with an internal carotid artery aneurysm and an anterior communicating artery aneurysm, based on the CT angiography findings of a patient. To simulate the formation of the anterior communicating artery aneurysm and the growth of the internal carotid artery aneurysm, we then constructed a model that virtually removed the anterior communicating artery aneurysm, and a further two models that also progressively decreased the size of the internal carotid artery aneurysm. Computational simulations of the fluid dynamics of the four models were performed under pulsatile flow conditions, and wall shear stress was compared among the different models. In the three aneurysm growth models, increasing size of the aneurysm was associated with an increased area of low wall shear stress, a significant decrease in wall shear stress at the dome of the aneurysm, and a significant change in the wall shear stress of the parent artery. The wall shear stress of the anterior communicating artery remained low, and was significantly lower than the wall shear stress at the bifurcation of the internal carotid artery or the bifurcation of the middle cerebral artery. After formation of the anterior communicating artery aneurysm, the wall shear stress at the dome of the internal carotid artery aneurysm increased significantly, and the wall shear stress in the upstream arteries also changed significantly. These findings indicate that low wall shear stress may be associated with the initiation and growth of aneurysms, and that aneurysm formation and growth may influence hemodynamic parameters in the local and adjacent arteries.

  15. DNA mismatch repair deficiency accelerates lung neoplasm development in K-rasLA1/+ mice: a brief report

    Inherited as well as acquired deficiencies in specific DNA mismatch repair (MMR) components are associated with the development of a wide range of benign and malignant neoplasms. Loss of key members such as MSH2 and MLH1 severely cripples the ability of the cell to recognize and correct such lesions as base:base mismatches and replicative DNA polymerase errors such as slippages at repetitive sequences. Genomic instability resulting from MMR deficiency not only predisposes cells to malignant transformation but may also promote tumor progression. To test the latter, we interbred Msh2−/− mice with the K-rasLA1/+ transgenic line that spontaneously develops a range of premalignant and malignant lung lesions. Compared to K-rasLA1/+mice, K-rasLA1/+; Msh2−/− mice developed lung adenomas and adenocarcinomas at an increased frequency and also demonstrated evidence of accelerated adenocarcinoma growth. Since MMR defects have been identified in some human lung cancers, the mutant mice may not only be of preclinical utility but they will also be useful in identifying gene alterations able to act in concert with Kras mutants to promote tumor progression

  16. Influence of BBO temperature distribution on phase mismatching distribution in fourth harmonic

    Wang, Chao; Yu, Miao; Chen, Xin-yu; Tan, Xue-chun; Yu, Yong-ji; Jin, Guang-yong; Wan, Chun-ming

    2013-09-01

    266nm UV laser has a wide range of applications in various fields by its advantages in high single photon energy and high resolution, which also has a development gradually moving in the direction of high power and high conversion efficiency. In the process of high-power laser frequency doubling, BBO crystal inevitably absorbs part of fundamental light power and frequency doubled light power, it induced the temperature rise along the direction of radiation in crystal and destroyed the phase-matching conditions of BBO crystal that lead to phase mismatching. In order to improve harmonic conversion efficiency as well as reduce the influence of output power and beam quality caused by phase mismatching, in this paper we analyzed the process of phase mismatching, established the thermal-induced phase mismatching model by using analytical expression of the nonlinear crystal temperature field equation which has been given, and the three-dimensional phase mismatching distribution were obtained. There are three major contributions in the paper. Firstly, the working process of the nonlinear crystal was analyzed, and the physical and mathematical models of temperature distributions were established, and the BBO crystal three-dimensional temperature distributions were also obtained. Secondly, a variety of factors that affect the temperature distributions within the BBO crystal were summarized. For different 532nm waist radius and 532nm input power, they were numerical simulated use of MATLAB. Finally, combined with the above analysis, the physical and mathematical models of phase mismatching caused by energy absorption of BBO in forth harmonics generation were established, the phase mismatching distributions in the crystal were simulated as well, especially the changes to phase mismatching distributions with different parameter were analyzed. Combination of the multiplier theory, the influence of phase mismatching on frequency doubling conversion efficiency was analyzed. The

  17. Hydrologic Connection Between Geysers and Adjacent Thermal Pools, Two Examples: El Tatio, Chile and Yellowstone, USA

    Munoz Saez, C.; Fauria, K.; Manga, M.; Hurwitz, S.; Namiki, A.

    2014-12-01

    Geyser eruption cycles can be influenced by adjacent and distant thermals sources, suggesting a hydraulic connection through permeable pathways. Diffusion of fluid pressure can be responsible for the communication between geysers. In this study we examine the processes linking two different geysers with adjacent thermal pools. The first was Vega Rinconada, located at El Tatio geyser field, Chile, where we measured temperature inside the conduit between the ground surface and a depth of seven meters, at one-meter intervals. The second was Lone Star Geyser in Yellowstone National Park, where we measured temperature of the overflow water at the base of the cone. Concurrently, we measured temperature and the water level in pools adjacent to both geysers. We found common elements in both geyser - pool systems: First, water temperature in both adjacent pools was below the boiling point and cooler than water in the geysers. Second, changes in pool water levels were correlated with eruptions of the geysers. During the quiescent period of the geysers, the water level increased in adjacent pools, while water level in the pools deceased during eruptions. Additionally, measurements inside of the conduit in Vega Rinconada Geyser showed that water temperature increased in the deepest part of the conduit during eruptions, while water temperature decreased in the shallow part of the geyser conduit (~1 to 2 m). These drops in temperature in the shallow conduit were coincident with the drop in water level in the adjacent pool. This suggests that after the initiation of an eruption, water may drain from the pool to the geyser. Furthermore, we observed a temperature drop of 3oC in the shallow conduit immediately preceding the end of an eruption. This suggests that flow from the pool to geyser contributes to eruption shut off. Our observations of geyser-pool systems indicate a hydrologic connection between the geysers and their adjacent pools. In the case of Vega Rinconada, cold water

  18. Late Permian to Triassic intraplate orogeny of the southern Tianshan and adjacent regions, NW China

    Wei Ju; Guiting Hou

    2014-01-01

    The South Tianshan Orogen and adjacent regions of Central Asia are located in the southwestern part of the Central Asian Orogenic Belt. The formation of South Tianshan Orogen was a diachronous, scissors-like process, which took place during the Palaeozoic, and its western segment was accepted as a site of the final collision between the Tarim Craton and the North Asian continent, which occurred in the late Palaeozoic. However, the post-collisional tectonic evolution of the South Tianshan Orogen and adjacent regions remains debatable. Based on previous studies and recent geochronogical data, we suggest that the final collision between the Tarim Craton and the North Asian continent occurred during the late Carboniferous. Therefore, the Permian was a period of intracontinental environment in the southern Tianshan and adjacent regions. We propose that an earlier, small-scale intraplate orogenic stage occurred in late Permian to Triassic time, which was the first intraplate process in the South Tianshan Orogen and adjacent regions. The later large-scale and well-known Neogene to Quaternary intraplate orogeny was induced by the collision between the India subcontinent and the Eurasian plate. The paper presents a new evolutionary model for the South Tianshan Orogen and adjacent regions, which includes seven stages: (I) late Ordovicianeearly Silurian opening of the South Tianshan Ocean;(II) middle Silurianemiddle Devonian subduction of the South Tianshan Ocean beneath an active margin of the North Asian continent; (III) late Devonianelate Carboniferous closure of the South Tianshan Ocean and collision between the Kazakhstan-Yili and Tarim continental blocks;(IV) early Permian post-collisional magmatism and rifting;(V) late PermianeTriassic the first intraplate orogeny;(VI) JurassicePalaeogene tectonic stagnation and (VII) NeoceneeQuaternary intraplate orogeny.

  19. Effects of refractive index mismatch in optical CT imaging of polymer gel dosimeters

    Manjappa, Rakesh; Makki S, Sharath; Kanhirodan, Rajan, E-mail: rajan@physics.iisc.ernet.in [Department of Physics, Indian Institute of Science, Bangalore 560012 (India); Kumar, Rajesh [Radiological Physics and Advisory Division, BARC, Mumbai 400094 (India)

    2015-02-15

    Purpose: Proposing an image reconstruction technique, algebraic reconstruction technique-refraction correction (ART-rc). The proposed method takes care of refractive index mismatches present in gel dosimeter scanner at the boundary, and also corrects for the interior ray refraction. Polymer gel dosimeters with high dose regions have higher refractive index and optical density compared to the background medium, these changes in refractive index at high dose results in interior ray bending. Methods: The inclusion of the effects of refraction is an important step in reconstruction of optical density in gel dosimeters. The proposed ray tracing algorithm models the interior multiple refraction at the inhomogeneities. Jacob’s ray tracing algorithm has been modified to calculate the pathlengths of the ray that traverses through the higher dose regions. The algorithm computes the length of the ray in each pixel along its path and is used as the weight matrix. Algebraic reconstruction technique and pixel based reconstruction algorithms are used for solving the reconstruction problem. The proposed method is tested with numerical phantoms for various noise levels. The experimental dosimetric results are also presented. Results: The results show that the proposed scheme ART-rc is able to reconstruct optical density inside the dosimeter better than the results obtained using filtered backprojection and conventional algebraic reconstruction approaches. The quantitative improvement using ART-rc is evaluated using gamma-index. The refraction errors due to regions of different refractive indices are discussed. The effects of modeling of interior refraction in the dose region are presented. Conclusions: The errors propagated due to multiple refraction effects have been modeled and the improvements in reconstruction using proposed model is presented. The refractive index of the dosimeter has a mismatch with the surrounding medium (for dry air or water scanning). The algorithm

  20. Group edge choosability of planar graphs without adjacent short cycles

    Xin ZHANG; Liu, Guizhen

    2011-01-01

    In this paper, we aim to introduce the group version of edge coloring and list edge coloring, and prove that all 2-degenerate graphs along with some planar graphs without adjacent short cycles is group $(\\Delta(G)+1)$-edge-choosable while some planar graphs with large girth and maximum degree is group $\\Delta(G)$-edge-choosable.

  1. PROBABILISTIC ZONING OF ADJACENT CATCHMENTS BY PHYSICAL MODELING

    Yu. B. Andreev

    2015-11-01

    Full Text Available Now the probabilistic zoning of avalanche sites is being made either on the basis of the available field data, or using the mathematical and physical modeling of avalanche dynamics processes. The work purpose consisted in research of interaction and compiling probabilistic zoning maps of two adjacent avalanche sites by a physical modeling method. For the two adjacent sites № 9 and 10 on the slope of northern exposition ofTchegetMountain(Elbrus region,Caucasus the model of this slope with the scale of 1: 2 500 was created. The model dimension is 0,82 × 1,1 ×0,57 m. This model was produced using the 4-mm plywood sheets imposed against each other. The model horizontals correspond to the10 mnatural ones. The model slope was also surfaced by thin gypsum lay and then varnished. The fine-grained sawdust (0.1 mm with a natural friction angle of 43º has been chosen as the loose material modeling snow. A series of experiments on modeling avalanche releases from the two sites were carried out resulted in the measurements of avalanche deposit parameters such as run-out distance and length, width and thickness of deposits. The similarity criteria were used during the experiments. The morphometric features of adjacent sites interaction are revealed. The basic result of the carried out work will consist in the probabilistic zoning map of two adjacent sites № 9 and 10.

  2. Biogeochemistry of hydrothermally and adjacent non-altered soils

    As a field/lab project, students in the Soil Biogeochemistry class of the University of Nevada, Reno described and characterized seven pedons, developed in hydrothermally and adjacent non-hydrothermally altered andesitic parent material near Reno, NV. Hydrothermally altered soils had considerably lo...

  3. The tiger beetles ( Coleoptera , Carabidae , Cicindelinae ) of Israel and adjacent lands

    Matalin, Andrey V.; Chikatunov, Vladimir I.

    2016-01-01

    Abstract Based on field studies, museums collections and literature sources, the current knowledge of the tiger beetle fauna of Israel and adjacent lands is presented. In Israel eight species occur, one of them with two subspecies, while in the Sinai Peninsula nine species of tiger beetles are now known. In the combined regions seven genera from two tribes were found. The Rift Valley with six cicindelids species is the most specious region of Israel. Cylindera contorta valdenbergi and Cicinde...

  4. The tiger beetles (Coleoptera, Carabidae, Cicindelinae) of Israel and adjacent lands

    Matalin,Andrey; I. Chikatunov,Vladimir

    2016-01-01

    Based on field studies, museums collections and literature sources, the current knowledge of the tiger beetle fauna of Israel and adjacent lands is presented. In Israel eight species occur, one of them with two subspecies, while in the Sinai Peninsula nine species of tiger beetles are now known. In the combined regions seven genera from two tribes were found. The Rift Valley with six cicindelids species is the most specious region of Israel. Cylindera contorta valdenbergi and Cicindela javeti...

  5. Myocardial perfusion/metabolism mismatch and ventricular arrhythmias in the chronic post infarction state

    Aim: Ventricular arrhythmias have been shown to originate in the myocardial peri-infarct region due to irregular heterotopic conduction. Hypoperfused but viable myocardium is often localised in those areas and may be involved in the pathogenesis of arrhythmias. We tested the hypothesis that these myocardial perfusion/metabolism mismatches (MM) are significantly associated with ventricular arrhythmias in the chronic post infarction state. Patients, methods: 47 post infarction patients were included in the study. 33 suffered from ventricular arrhythmia whereas 14 did not. All patients underwent 99mTc tetrofosmin SPECT and 18F-FDG PET. A region-of-interest(ROI)-analysis was used to assess viable myocardium based on predefined MM-criteria. Univariate analyses as well as a logistic regression model for the multivariate analysis were carried out. Results: 94% of the arrhythmic patients displayed at least one MM-segment as compared to 64% of the non-arrhythmic patients. MM-segments and arrhythmia showed a statistically significant relation (p=0.018). The logistic regression model predicted the occurrence or absence of arrhythmia in 85% of all cases. Multivariate analysis gave consistent results, after adjusting for symptomatic chronic heart failure (CHF), aneurysms and age. Conclusion: Our results support the hypothesis that hypoperfused but viable myocardium represents an arrhyhmogenic substrate and is a relevant risk factor for developing ventricular arrhythmias following myocardial infarction. Therefore, the detection of MM-segments allows the identification of patients with a higher risk for future cardiac events. (orig.)

  6. Mlh2 is an accessory factor for DNA mismatch repair in Saccharomyces cerevisiae.

    Christopher S Campbell

    2014-05-01

    Full Text Available In Saccharomyces cerevisiae, the essential mismatch repair (MMR endonuclease Mlh1-Pms1 forms foci promoted by Msh2-Msh6 or Msh2-Msh3 in response to mispaired bases. Here we analyzed the Mlh1-Mlh2 complex, whose role in MMR has been unclear. Mlh1-Mlh2 formed foci that often colocalized with and had a longer lifetime than Mlh1-Pms1 foci. Mlh1-Mlh2 foci were similar to Mlh1-Pms1 foci: they required mispair recognition by Msh2-Msh6, increased in response to increased mispairs or downstream defects in MMR, and formed after induction of DNA damage by phleomycin but not double-stranded breaks by I-SceI. Mlh1-Mlh2 could be recruited to mispair-containing DNA in vitro by either Msh2-Msh6 or Msh2-Msh3. Deletion of MLH2 caused a synergistic increase in mutation rate in combination with deletion of MSH6 or reduced expression of Pms1. Phylogenetic analysis demonstrated that the S. cerevisiae Mlh2 protein and the mammalian PMS1 protein are homologs. These results support a hypothesis that Mlh1-Mlh2 is a non-essential accessory factor that acts to enhance the activity of Mlh1-Pms1.

  7. Optical signature of multicellular tumor spheroid using index-mismatch-induced spherical aberrations

    Le Corre, G.; Weiss, P.; Ducommun, B.; Lorenzo, C.

    2014-02-01

    The development of new cancer treatments and the early prediction of their therapeutic potential are often made difficult by the lack of predictive pharmacological models. The 3D multicellular tumor spheroid (MCTS) model offers a level of complexity that recapitulates the three-dimensional organization of a tumor and appears to be fairly predictive of therapeutic efficiency. The use of spheroids in large-scale automated screening was recently reported to link the power of a high throughput analysis to the predictability of a 3D cell model. The spheroid has a radial symmetry; this simple geometry allows establishing a direct correlation between structure and function. The outmost layers of MCTS are composed of proliferating cells and form structurally uniform domain with an approximate thickness of 100 microns. The innermost layers are composed of quiescent cells. Finally, cells in the center of the spheroid can form a necrotic core. This latest region is structurally heterogeneous and is poorly characterized. These features make the spheroid a model of choice and a paradigm to study the optical properties of various epithelial tissues. In this study, we used an in-vitro optical technique for label-free characterization of multicellular systems based on the index- mismatch induced spherical aberrations. We achieve to monitor and characterize the optical properties of MCTS. This new and original approach might be of major interest for the development of innovative screening strategies dedicated to the identification of anticancer drugs.

  8. Mismatch negativity to the patient's own name in chronic disorders of consciousness.

    Qin, Pengmin; Di, Haibo; Yan, Xiaodan; Yu, Senming; Yu, Dan; Laureys, Steven; Weng, Xuchu

    2008-12-19

    Previous studies implicated potential value of mismatch negativity (MMN) in predicting recovery of consciousness in patients with disorders of consciousness (DOC). We have adopted a novel MMN evoked by subject's own name (SON), a self-referential stimulus thought to be powerful in evoking residual brain activity, and examined the correlation between the MMN and recovery of consciousness in patients with chronic (>1 month) DOC. Twelve patients and 12 age-matched healthy controls were investigated. The patients were diagnosed as coma (n=4), vegetative state (VS, n=6), and minimally conscious state (MCS, n=2), mainly based on the JFK Coma Recovery Scale-Revised. The SON-evoked MMN (SON-MMN) was present in seven patients. Critically, the presence of SON-MMN was significantly correlated with recovery of consciousness. While four of the five patients (three VS and two coma) showing SON-MMN changed to MCS 3 months later, the rest of the patients (three VS and two coma) without SON-MMN failed to show any clinical improvement. Our study thus illustrates that the subject's own name is effective in evoking MMN in patients with DOC, and that SON-MMN has potential prognostic values in predicting recovery of consciousness. PMID:18938213

  9. Effect of Notch Location on Fatigue Life Prediction of Strength Mismatched HSLA Steel Weldments

    S. Ravi; V. Balasubramanian; S. Nemat Nasser

    2004-01-01

    Welding of high strength low alloy steels (HSLA) involves usage of Iow, even and high strength filler materials (electrodes) than the parent material depending on the application of the welded structures and the availability of the filler material. In the present investigation, the fatigue crack growth behaviour of weld metal (WM) and heat affected zone (HAZ) regions of under matched (UM), equal matched (EM) and over matched (OM)joints has been studied. The base material used in this investigation is HSLA-80 steel of weldable grade. Shielded metal arc welding (SMAW) process has been used to fabricate the butt joints. Centre cracked tension (CCT) specimen has been used to evaluate the fatigue crack growth behaviour of the welded joints. Fatigue crack growth experiments have been conducted using servo hydraulic controlled fatigue testing machine at constant amplitude loading (R=0). A method has been proposed to predict the fatigue life of HSLA steel welds using fracture mechanics approach by incorporating influences of mismatch ratio (MMR) and notch location.

  10. A DDES model with a Smagorinsky-type eddy viscosity formulation and log-layer mismatch correction

    Highlights: • An alternate DDES formulation is proposed via the eddy viscosity definition. • Eddy viscosity is expressed as a Smagorinsky-type formula. • Log-layer mismatch is corrected by changing the length scale definition. • Model is validated for 2D as well as 3D flows. - Abstract: The current work develops a variant of delayed detached eddy simulation (DDES) that could be characterized as limiting the production term. Previous formulations have been based on limiting the dissipation rate (Spalart et al., 2006). A clipped length scale is applied directly to the eddy viscosity, yielding a Smagorinsky-like formulation when the model is on the eddy simulation branch. That clipped eddy viscosity limits the production rate. The length scale is modified in order to account for the log-layer mismatch (a well-known issue with DDES), without using additional blending functions. Another view of our approach is that the subgrid eddy-viscosity is represented by a mixing length formula l2ω; in the eddy field ω acts like a filtered rate of strain. Our model is validated for channel flow as well as separated flows (backward-facing step, 2D periodic hills) and illustrated via an air-blast atomizer

  11. A simple and rapid quantitative method of detection of the common achondroplasia mutation: Analysis in mismatch repair deficient cells

    Grewal Raji

    2004-01-01

    Full Text Available Achondroplasia is the most common form of dwarfism and has an incidence of approximately 1/7,500. In more than 97% of cases, it is caused by a recurrent point mutation, a G to A substitution at nucleotide position 1138 (G1138A of the fibroblast growth factor receptor 3 gene. Although this is an autosomal dominant condition, more than 90% of all mutations occur sporadically making this one of the most mutagenic sites in the human genome. The reasons for the high spontaneous G1138A mutation rate are not known. This investigation was performed by developing a simple and rapid semi-quantitative allele specific PCR based assay capable of reliably detecting more than 25 mutant G1138A copies in a pool of 300,000 wild type molecules. Using this assay, the G1138A mutation frequency was measured in cell lines deficient in mismatch repair (LoVo, SW48 and comparing it with controls. No differences were found in the frequency of this point mutation between the mismatch repair deficient and wild type cell lines.

  12. Bronchopulmonary dysplasia: clinical grading in relation to ventilation/perfusion mismatch measured by single photon emission computed tomography.

    Kjellberg, Malin; Björkman, Karin; Rohdin, Malin; Sanchez-Crespo, Alejandro; Jonsson, Baldvin

    2013-12-01

    Bronchopulmonary dysplasia (BPD) is a significant cause of morbidity in the preterm population. Clinical severity grading based on the need for supplemental oxygen and/or need for positive airway pressure at 36 weeks postmenstrual age does not yield reproducible predictive values for later pulmonary morbidity. Single photon emission computed tomography (SPECT) was used to measure the distribution of lung ventilation (V) and perfusion (Q) in 30 BPD preterm infants at a median age of 37 weeks postmenstrual age. The V and Q were traced with 5 MBq Technegas and Technetium-labeled albumin macro aggregates, respectively, and the V/Q match-mismatch was used to quantify the extent of lung function impairment. The latter was then compared with the clinical severity grading at 36 weeks, and time spent on mechanical ventilation, continuous positive airway pressure (CPAP) and supplemental oxygen. Of those with mild and moderate BPD 3/9 and 3/11 patients, respectively, showed significant V/Q mismatches. By contrast, 4/10 patients with severe BPD showed a satisfactory V/Q matching distribution. An unsatisfactory V/Q match was not correlated with time spent on supplemental oxygen or CPAP, but was significantly negatively correlated with time spent on mechanical ventilation. SPECT provides unique additional information about regional lung function. The results suggest that the current clinical severity grading can be improved and/or complemented with SPECT. PMID:23359534

  13. Probe-beam diffraction in a pulsed top-hat beam thermal lens with a mode-mismatched configuration.

    Li, B; Welsch, E

    1999-08-20

    The Fresnel diffraction integral is used directly to describe the thermal lens (TL) effect with a mode-mismatched collinear configuration. The TL amplitudes obtained with Gaussian, Airy, and top-hat beam excitations are computed and compared. Numerical results for beam geometries optimized for both near- and far-field detection schemes are presented, and the analytical results developed by Bialkowski and Chartier [Appl. Opt. 36, 6711 (1997)] for a Gaussian beam TL effect are summarized in simplified form. Both the numerical and the analytical results demonstrate that, under a beam geometry optimized for either near- or far-field detection, the Gaussian beam TL experiment has approximately the same maximum signal amplitude as does the photothermal-interference scheme. A comparison between the optimum near- and far-field detection beam geometries indicates that a practical mode-mismatched TL instrument should be based on the far-field detection geometry. The computation results further demonstrate that the optimum beam geometry and the TL amplitude depend largely on the excitation-beam profile. The top-hat beam TL experiment is approximately twice as sensitive as the Gaussian beam TL scheme. PMID:18324024

  14. Role of energy-level mismatches in a multi-pathway complex of photosynthesis

    Considering a multi-pathway structure in a light-harvesting complex of photosynthesis, we investigated the role of energy-level mismatches between antenna molecules in transferring the absorbed energy to a reaction center (RC). We found a condition in which the antenna molecules faithfully play their roles: when their effective absorption ratios are larger than those of the receiver molecule directly coupled to the RC. In the absence of energy-level mismatches and dephasing noise, there arises quantum destructive interference between multiple paths that restricts the energy transfer. On the other hand, the destructive interference diminishes as asymmetrically biasing the energy-level mismatches and/or introducing quantum noise of dephasing for the antenna molecules, so that the transfer efficiency is greatly enhanced to nearly unity. Remarkably, the near-unity efficiency can be achieved at a wide range of asymmetric energy-level mismatches. Temporal characteristics are also optimized at the energy-level mismatches where the transfer efficiency is nearly unity. We discuss these effects, in particular, for the Fenna-Matthews-Olson complex. (paper)

  15. Recommended practice for fracture toughness testing of weldments with strength mismatch

    Fracture toughness testing requires relationships between experimentally measured quantities, such as load and displacement, and J and crack tip opening displacement (CTOD).The relationships provided in the presently codified procedures (ASTM E813, E1152, E1290 et BSI 7848:Part 1) were derived under the assumption that the specimens have homogeneous mechanical properties. However, these codified procedures are frequently used for testing of weldments despite their strong mechanical properties variations. As a result, the accuracy of the toughness values (J or CCTOD) is sometimes in question. Systematic finite element studies of mismatched single-edge-notched-bend specimens (SENB) having a crack on the centerline have been conducted to resolve this question. The effect of various parameters on these relationships, such as weld size, degree of mismatch, and crack depth, is investigated. The accuracy of the codified J and CTOD testing procedures when applied to the mismatched SE(B) specimens is examined. This systematic examination is extended to several newly proposed procedures, such as those from Joch et al. and Hornet and Eripret. New J and CTOD estimations procedures are proposed. The expected error in applying the codified and the new procedures are shown are proposed. The expected error in applying the codified and the new procedures are shown in terms of mismatch level and level width. Recommendations are made on the use of those procedures for a variety of weld mismatch and crack depth conditions. (authors)

  16. Molecular dynamics simulation of thin film interfacial strength dependency on lattice mismatch

    Laser-induced thin film spallation experiments have been previously developed to characterize the intrinsic interfacial strength of thin films. In order to gain insights of atomic level thin film debonding processes and the interfacial strength dependence on film/substrate lattice structures, in this study, molecular dynamics simulations of thin film interfacial failure under laser-induced stress waves were performed. Various loading amplitudes and pulse durations were employed to identify the optimum simulation condition. Stress propagation as a function of time was revealed in conjunction with the interface structures. Parametric studies confirmed that while the interfacial strength between a thin film and a substrate does not depend on the film thickness and the duration of the laser pulse, a thicker film and a shorter duration do provide advantage to effectively load the interface to failure. With the optimized simulation condition, further studies were focused on bulk Au/Au bi-crystals with mismatched orientations, and Ni/Al, Cu/Al, Cu/Ag and Cu/Au bi-crystals with mismatched lattices. The interfacial strength was found to decrease with increasing orientation mismatch and lattice mismatch but more significantly dominated by the bonding elements' atomic structure and valence electron occupancy. - Highlights: • Molecular dynamics simulation was done on stress wave induced thin film spallation. • Atomic structure was found to be a primary strength determining factor. • Lattice mismatch was found to be a secondary strength determining factor

  17. Evaluation of diffusion-perfusion mismatch for determining indication for emergency endovascular revascularization

    We evaluated the usefulness of assessing by diffusion-perfusion mismatch (D/P mismatch) whether there is adaptation of neuroendovascular revascularization for acute ischemic stroke out of intravenous tissue plasminogen activator (IV t-PA). We retrospectively analyzed 24 patients who underwent D/P mismatch and endovascular treatment between October 2005 and September 2008. This investigation included stroke patients with a National Institutes of Health Stroke Scale (NIHSS) score less than 4. Sixteen acute ischemic stroke patients had an NIHSS score greater than 5. Eight patients (50%) had a favorable neurological outcome (modified Rankin Scale 0 to 2). Eight acute ischemic stroke patients had an NIHSS score equal to or less than 4. Four patients who underwent emergency endovascular treatment on admission had a favorable neurological outcome, but 3 patients treated for progressive stroke after admission all had a poor prognosis. Evaluating D/P mismatch was useful for determining the adaptation of emergency neuroendovascular revascularization for acute ischemic stroke out of IV t-PA. Acute ischemic stroke patients with an NIHSS score equal to or less than 4 and diffusion/perfusion mismatch need careful observation to enable endovascular treatment immediately after progressive stroke. (author)

  18. Switched-capacitor multiply-by-two amplifier with reduced capacitor mismatches sensitivity and full swing sample signal common-mode voltage

    A switched-capacitor amplifier with an accurate gain of two that is insensitive to component mismatch is proposed. This structure is based on associating two sets of two capacitors in cross series during the amplification phase. This circuit permits the common-mode voltage of the sample signal to reach full swing. Using the charge-complement technique, the proposed amplifier can reduce the impact of parasitic capacitors on the gain accuracy effectively. Simulation results show that as sample signal common-mode voltage changes, the difference between the minimum and maximum gain error is less than 0.03%. When the capacitor mismatch is increased from 0 to 0.2%, the gain error is deteriorated by 0.00015%. In all simulations, the gain of amplifier is 69 dB. (semiconductor integrated circuits)

  19. Application of the simplified J-estimation scheme Aramis to mismatching welds in CCP; Application du concept d`integrale J dans l`outil Aramis aux effets de mismatch sur des eprouvettes CCP

    Eripret, C.; Franco, C.; Gilles, P.

    1995-12-31

    The J-based criteria give reasonable predictions of the failure behaviour of ductile cracked metallic structures, even if the material characterization may be sensitive to the size of the specimens. However in cracked welds, this phenomenon due to stress triaxiality effects could be enhanced. Furthermore, the application of conventional methods of toughness measurement (ESIS or ASTM standard) have evidenced a strong influence of the portion of the weld metal in the specimen. Several authors have shown the inadequacy of the simplified J-estimation methods developed for homogeneous materials. These heterogeneity effects mainly related to the mismatch ratio (ratio of weld metal yield strength upon base metal yield strength) as well as to the geometrical parameter h/W-a (weld width upon ligament size). In order to make decisive progress in this field, the Atomic Energy Commission (CEA), the PWR manufacturer FRAMATOME, and the French utility (EDF) have launched a large research program on cracked piping welds behaviour. As part of this program, a new J-estimation scheme, so called ARAMIS, has been developed to account for the influence of both materials, i.e. base metal and weld metal, on the structural resistance of cracked welds. It has been shown that, when the mismatch is high, and when the ligament size is small compared to the weld width, a classical J-based method using the softer material properties is very conservative. On the opposite the ARAMIS method provides a good estimate of J, because it predicts pretty well the shift of the cracked weld limit load, due to the presence of the weld. the influence of geometrical parameters such as crack size, weld width, or specimen length is property accounted for. (authors). 23 refs., 8 figs., 1 tab., 1 appendix.

  20. The relative effects of age and learning style mismatch on adult students' academic achievement and perception of instructors

    Garrett, Clayton W.

    1996-01-01

    This study investigated the relationship between students' age, achievement, evaluation of the instructors and the match-mismatch of students' and instructors' learning styles. Seventeen (17) business instructors and 302 business students comprised the population. The students were selected as an intact group enrolled in the participating faculty members' class. The relationship between age and learning style mismatch and evaluation and age and learning style mismatch an...

  1. Teaching Fillers, Discourse Markers and Adjacency Pairs to College Stu-dents

    王蓓

    2015-01-01

    Speaking has long been underestimated in College English education, compared with reading , writing and listening. Even In universities where spoken courses are provided, verbal filler and discourse markers which vastly exist in native speaker ’s corpora are rarely found in these prescribed spoken course books. For the teaching of adjacency pairs, which are always one of the focuses in teaching conversation skills in College English spoken program ,students are required to make up a new one based on the given scenario after they have memorized model dialogues. Unfortunately, students always experience pragmatic difficulty when they interact with people in the real world if they are guided by this approach. This article suggests a triangle which is com⁃posed of exposure, instruction and practice to teach verbal filler, discourse markers and adjacency pairs. This triangle combines both direct approach and indirect approach. Furthermore, in spoken program , English teachers can adapt spoken course books to integrate features of authentic conversations.

  2. Crucial problems on security assessment of a building site adjacent to an excavated high slope

    文海家; ZHANG; Jialan; 等

    2002-01-01

    The subject of this work is the assessment on the stability of an excavated high slope in order to insure the security of the building site adjacent to the slope,which is frequently encountered in town construction in mountainous areas due to terrain limit.On the base of some typical engineering cases in Chongqing,several crucial problems on security assessment of building site adjacent to an excavated high slope,including the natural geological conditions and man-destroyed degree,engineering environment,potential failure pattern of the high slope,calculation parameters and analysis methods,are roundly discussed.It is demonstrated that the conclusion of security assessment can be determined according to the aspects above-mentioned,and the security assessment is one of the fundamental data to insure the safety of the related construction,site and buildings.

  3. A sea temperature data assimilation system for the China Seas and adjacent areas

    YOU Xiaobao; ZHOU Guangqing; ZHU Jiang; LI Rongfeng

    2003-01-01

    A sea temperature data assimilation system for the China Seas and adjacent areas is developed based on a nested regional ocean circulation model and variational optimal interpolation assimilation method. A 12-year assimilation experiment is performed by using the observational temperature profiles from World Ocean Database 1998 (WOD98) and ECMWF reanalysis surface wind stress. Experimental results indicate that the variational scheme shows good skill in assimilating the observed sea temperature into a regional ocean circulation model. Compared with simulation alone, the sea temperature data assimilation significantly improves the performance of a regional ocean model and obtains comprehensive description of the circulations in the China Seas and adjacent areas. Assimilation results, such as the strength and flowing axis of the Kuroshio, Yellow Sea Cold Water Mass and its corresponding horizontal circulation in summer and dipole eddies in the South China Sea in the late summer/early autumn and the eastward jet between them, are well consistent with the observed evidences.

  4. Steganography in 3D Geometries and Images by Adjacent Bin Mapping

    Hao-Tian Wu

    2009-01-01

    Full Text Available A steganographic method called adjacent bin mapping (ABM is presented. Firstly, it is applied to 3D geometries by mapping the coordinates within two adjacent bins for data embedding. When applied to digital images, it becomes a kind of LSB hiding, namely the LSB+ algorithm. In order to prevent the detection using a metric named histogram tail, the hiding is performed in a pseudorandom order. Then we show that the steganalytic algorithms based on histogram characteristic function (HCF can be prevented by implementing the LSB+ algorithm on subsets of pixels having the same neighbor values. The experimental results show that important high-order statistics of the cover image are preserved in this way while little distortion is introduced to 3D geometric models with an appropriate bin size.

  5. Steganography in 3D Geometries and Images by Adjacent Bin Mapping

    Wu Hao-Tian

    2009-01-01

    Full Text Available A steganographic method called adjacent bin mapping (ABM is presented. Firstly, it is applied to 3D geometries by mapping the coordinates within two adjacent bins for data embedding. When applied to digital images, it becomes a kind of LSB hiding, namely the algorithm. In order to prevent the detection using a metric named histogram tail, the hiding is performed in a pseudorandom order. Then we show that the steganalytic algorithms based on histogram characteristic function (HCF can be prevented by implementing the algorithm on subsets of pixels having the same neighbor values. The experimental results show that important high-order statistics of the cover image are preserved in this way while little distortion is introduced to 3D geometric models with an appropriate bin size.

  6. Research on the Supply-Demand Mismatch of Pension Services for the Disabled Elderly in China---Based on the Survey Data in Nine Provinces%中国老年残疾人养老服务供需问题研究--基于9省调查问卷的分析

    徐宏

    2015-01-01

    为深入了解我国老年残疾人养老服务的供需现状以及存在的问题,对全国9省2068份问卷调查结果进行分析,结果发现残疾人养老服务供需矛盾突出,老年残疾人养老服务工作存在养老服务模式供给与实际需求不匹配、养老服务社会化和专业化程度低、养老服务人员供给不足等问题。针对以上问题,建议通过社会参与、项目管理、整合老年残疾人养老服务资金来源等措施促进老年残疾人养老服务工作的“标准化、社会化和市场化”,以化解老年残疾人养老服务供需矛盾。%The survey data from 2068 questionnaires in 9 provinces in China are analyzed so as to get in-depth knowledge about the demand-supply situation and existing problems of the pension service for China’s disabled elderly. A conclusion is reached showing the outstanding contradiction between the supply and demand of the pension services for the disabled elderly. Such problems exist as the mismatch between the demand and supply of the modes of pension services,the inferiority in level of socialization and specialization of the pension services,and the insufficient supply of staffing. In view of the above problems,this paper puts forward suggestions that to resolve these contradictions,the pension services for the disabled elderly should be "standardized,socialized and marketized"through social participation,project management,and the integration of the capital source of the pension services for the disabled elder-ly.

  7. Improvement parameters in dynamic compaction adjacent to the slopes

    Elham Ghanbari

    2015-04-01

    Full Text Available Dynamic compaction is a cost-effective method commonly used for improvement of sandy soils. A number of researchers have investigated experimentally and numerically the improvement parameters of soils using dynamic compaction, such as crater depth, improvement depth, and radial improvement, however, these parameters are not studied for improvement adjacent to the slopes or trenches. In this research, four different slopes with different inclinations are modeled numerically using the finite element code ABAQUS, and impact loads of dynamic compaction are applied. The static factors of safety are kept similar for all trenches and determined numerically by application of gravity loads to the slope using strength reduction method (SRM. The analysis focuses on crater depth and improvement region which are compared to the state of flat ground. It can be observed that compacted area adjacent to the slopes is narrower and slightly away from the slope compared to the flat state. Moreover, crater depth increases with increase in slope inclination.

  8. Resonance-like tunneling across a barrier with adjacent wells

    S Mahadevan; P Prema; S K Agarwalla; B Sahu; C S Shastry

    2006-09-01

    We examine the behavior of transmission coefficient across the rectangular barrier when attractive potential well is present on one or both sides and also the same is studied for a smoother barrier with smooth adjacent wells having Woods–Saxon shape. We find that presence of well with suitable width and depth can substantially alter at energies below the barrier height leading to resonant-like structures. In a sense, this work is complementary to the resonant tunneling of particles across two rectangular barriers, which is being studied in detail in recent years with possible applications in mind. We interpret our results as due to resonant-like positive energy states generated by the adjacent wells. We describe in detail the possible potential application of these results in electronic devices using n-type oxygen-doped gallium arsenide and silicon dioxide. It is envisaged that these results will have applications in the design of tunneling devices.

  9. Regional Fault Systems of Qaidam Basin and Adjacent Orogenic Belts

    2003-01-01

    The purpose of this paper is to analyze the regional fault systems of Qaidam basin and adjacent orogenic belts. Field investigation and seismic interpretation indicate that five regional fault systems occurred in the Qaidam and adjacent mountain belts, controlling the development and evolution of the Qaidam basin. These fault systems are: (1)north Qaidam-Qilian Mountain fault system; (2) south Qaidam-East Kunlun Mountain fault system; (3)Altun strike-slip fault system; (4)Elashan strike-slip fault system, and (5) Gansen-Xiaochaidan fault system. It is indicated that the fault systems controlled the orientation of the Qaidam basin, the formation and distribution of secondary faults within the basin,the migration of depocenters and the distribution of hydrocarbon accumulation belt.

  10. On heavy machines dynamical loading under adjacent links shocks

    Трубачев, Сергей Иванович; Лысюк, Дмитрий Степанович; Талимонова, Ольга Юрьевна

    2014-01-01

    The mathematical model, describing the dynamics under collision of heavy machinery adjacent links in the presence of gaps and other elements with a dead or smoothness zone of transfer functions of kinematic chains, is given. The model is designed using a procedure of "smoothing" discontinuous functions and regarding a variable structure. The specific example of a dynamic system and its numerical calculation is considered. The pressure between the roller and a workpiece is expressed by means o...

  11. Acyclic Edge Coloring of Planar Graphs without Adjacent Triangles

    Dezheng XIE; Yanqing WU

    2012-01-01

    An acyclic edge coloring of a graph G is a proper edge coloring such that there are no bichromatic cycles.The acyclic edge chromatic number of a graph G is the minimum number k such that there exists an acyclic edge coloring using k colors and is denoted by x'a(G).In this paper we prove that x'a(G)≤ Δ(G)+ 5 for planar graphs G without adjacent triangles.

  12. Concentric network symmetry grasps authors' styles in word adjacency networks

    Amancio, Diego R.; Filipi N. Silva; Costa, Luciano da F.

    2015-01-01

    Several characteristics of written texts have been inferred from statistical analysis derived from networked models. Even though many network measurements have been adapted to study textual properties at several levels of complexity, some textual aspects have been disregarded. In this paper, we study the symmetry of word adjacency networks, a well-known representation of text as a graph. A statistical analysis of the symmetry distribution performed in several novels showed that most of the wo...

  13. FMRI evidence of acupoints specificity in two adjacent acupoints.

    Liu, Hua; Xu, Jian-Yang; Li, Lin; Shan, Bao-Ci; Nie, Bin-Bin; Xue, Jing-Quan

    2013-01-01

    Objectives. Acupoint specificity is the foundation of acupuncture treatment. The aim of this study is to investigate whether the acupoint specificity exists in two adjacent acupoints. Design and Setting. Two adjacent real acupoints, LR3 (Taichong) and ST44 (Neiting), and a nearby nonacupoint were selected. Thirty-three health volunteers were divided into three groups in random order, and each group only received acupuncture at one of the three points. While they received acupuncture, fMRI scan was performed. Results. The common cerebral activated areas responding to LR3 and ST44 included the contralateral primary somatosensory area (SI) and ipsilateral cerebellum. Acupuncture at LR3 specifically activated contralateral middle occipital gyrus, ipsilateral medial frontal gyrus, superior parietal lobe, middle temporal gyrus, rostral anterior cingulate cortex (rACC), lentiform nucleus, insula, and contralateral thalamus. Stimulation at ST44 selectively activated ipsilateral secondary somatosensory area (SII), contralateral middle frontal gyrus, inferior frontal gyrus, lingual gyrus, lentiform nucleus, and bilateral posterior cingulate cortex (PCC). Conclusions. Acupuncture at adjacent acupoints elicits distinct cerebral activation patterns, and those specific patterns might be involved in the mechanism of the specific therapeutic effects of different acupoints. PMID:23762172

  14. Trophic mismatch and its effects on the growth of young in an Arctic herbivore.

    Doiron, Madeleine; Gauthier, Gilles; Lévesque, Esther

    2015-12-01

    In highly seasonal environments, timing of breeding of organisms is typically set to coincide with the period of highest resource availability. However, breeding phenology may not change at a rate sufficient to keep up with rapid changes in the environment in the wake of climate change. The lack of synchrony between the phenology of consumers and that of their resources can lead to a phenomenon called trophic mismatch, which may have important consequences on the reproductive success of herbivores. We analyzed long-term data (1991-2010) on climate, plant phenology and the reproduction of a long-distance Arctic migrant, the greater snow goose (Chen caerulescens atlantica), in order to examine the effects of mismatched reproduction on the growth of young. We found that geese are only partially able to adjust their breeding phenology to compensate for annual changes in the timing of high-quality food plants, leading to mismatches of up to 20 days between the two. The peak of nitrogen concentration in plants, an index of their nutritive quality for goslings, occurred earlier in warm springs with an early snow melt. Likewise, mismatch between hatch dates of young and date of peak nitrogen was more important in years with early snow melt. Gosling body mass and structural size at fledging was reduced when trophic mismatch was high, particularly when the difference between date of peak nitrogen concentration and hatching was >9 days. Our results support the hypothesis that trophic mismatch can negatively affect the fitness of Arctic herbivores and that this is likely to be exacerbated by rising global temperatures. PMID:26235037

  15. Idiopathic pulmonary fibrosis. A rare cause of scintigraphic ventilation-perfusion mismatch

    A case of idiopathic pulmonary fibrosis with multiple areas of mismatch on ventilation-perfusion lung imaging in the absence of pulmonary embolism is presented. Idiopathic pulmonary fibrosis is one of the few nonembolic diseases producing a pulmonary ventilation-perfusion mismatch. In this condition, chest radiographs may not detect the full extent of disease, and xenon-133 ventilation imaging may be relatively insensitive to morbid changes in small airways. Thus, when examining patients with idiopathic pulmonary fibrosis, one should be aware that abnormal perfusion imaging patterns without matching ventilation abnormalities are not always due to embolism. In this setting, contrast pulmonary angiography is often needed for accurate differential diagnosis

  16. New functional sites in MutS affect DNA mismatch repair

    2010-01-01

    The MutS protein plays an important role in the DNA mismatch repair system. Mutations in the mutS gene can lead to genome instability and ultimately cell malfunction. Here we have established a method for identifying functional defective mutants of MutS by random mutation and rifampicin screening. Some novel functional sites in MutS were identified. The MutS mutant strains were analyzed using surface plasmon resonance, gel filtration and far-western methods to determine the molecular mechanisms behind the DNA mismatch repair function of MutS.

  17. Mismatch Correction Modulates Mutation Frequency and Pilus Phase and Antigenic Variation in Neisseria gonorrhoeae ▿

    Criss, Alison K.; Bonney, Kevin M.; Chang, Rhoda A.; Duffin, Paul M.; LeCuyer, Brian E.; Seifert, H. Steven

    2009-01-01

    The mismatch correction (MMC) system repairs DNA mismatches and single nucleotide insertions or deletions postreplication. To test the functions of MMC in the obligate human pathogen Neisseria gonorrhoeae, homologues of the core MMC genes mutS and mutL were inactivated in strain FA1090. No mutH homologue was found in the FA1090 genome, suggesting that gonococcal MMC is not methyl directed. MMC mutants were compared to a mutant in uvrD, the helicase that functions with MMC in Escherichia coli....

  18. Mouth reversal extinguishes mismatch negativity induced by the McGurk illusion

    Eskelund, Kasper; Andersen, Tobias

    2013-01-01

    cortex. Mismatch Negativity (MMN) is a component in the auditory Event-Related Potential (ERP) that is elicited by a change in the auditory percept. It has been shown that the McGurk illusion can induce a MMN. We conducted an experiment in which the MMN could be elicited by the McGurk illusion induced by...... visual speech with either upright (unaltered) or vertically reversed mouth area. In a preliminary analysis, we found a Mismatch Negativity component induced by the McGurk illusion for 6 of 17 participants at electrode Cz when the mouth area was upright. In comparison, these participants produced no...

  19. Idiopathic pulmonary fibrosis. A rare cause of scintigraphic ventilation-perfusion mismatch

    Pochis, W.T.; Krasnow, A.Z.; Collier, B.D.; Mewissen, M.W.; Almagro, U.A.; Hellman, R.S.; Isitman, A.T. (Medical College of Wisconsin, Milwaukee (USA))

    1990-05-01

    A case of idiopathic pulmonary fibrosis with multiple areas of mismatch on ventilation-perfusion lung imaging in the absence of pulmonary embolism is presented. Idiopathic pulmonary fibrosis is one of the few nonembolic diseases producing a pulmonary ventilation-perfusion mismatch. In this condition, chest radiographs may not detect the full extent of disease, and xenon-133 ventilation imaging may be relatively insensitive to morbid changes in small airways. Thus, when examining patients with idiopathic pulmonary fibrosis, one should be aware that abnormal perfusion imaging patterns without matching ventilation abnormalities are not always due to embolism. In this setting, contrast pulmonary angiography is often needed for accurate differential diagnosis.

  20. Impact of human leukocyte antigen mismatching on outcomes of liver transplantation:A meta-analysis

    2010-01-01

    AIM:To assess the effect of human leukocyte antigen(HLA) mismatching on liver graft outcome and acute rejection from a meta-analysis of available cohort studies.METHODS:Articles in PubMed/MEDLINE,EMBASE and the Cochrane database from January 1970 to June 2009,including non-English literature identified in these databases,were searched.Only studies comparing HLA or sub-phenotype matching with mismatching were extracted.The percentage of graft survival was extracted by "Engauge Digitizer" from survival curves...

  1. Dynamical Allosterism in the Mechanism of Action of DNA Mismatch Repair Protein MutS

    Pieniazek, Susan N.; Hingorani, Manju M.; Beveridge, D.L.

    2011-01-01

    The multidomain protein Thermus aquaticus MutS and its prokaryotic and eukaryotic homologs recognize DNA replication errors and initiate mismatch repair. MutS actions are fueled by ATP binding and hydrolysis, which modulate its interactions with DNA and other proteins in the mismatch-repair pathway. The DNA binding and ATPase activities are allosterically coupled over a distance of ∼70 Å, and the molecular mechanism of coupling has not been clarified. To address this problem, all-atom molecul...

  2. Functional angiocoupling between follicles and adjacent corpus luteum in heifers.

    Ginther, O J; Siddiqui, M A R; Baldrighi, J M

    2016-07-15

    In single ovulating cattle, ipsilateral versus contralateral interovarian relationships refer to a dominant follicle (DF) and CL in the same versus opposite ovaries. The ipsilateral relationship consists of the DF-CL and the devoid (no DF or CL) intraovarian pattern, and the contralateral relationship consists of the DF pattern and the CL pattern. The DF-CL pattern involves positive effects on both the DF and CL when adjacent (≤3-mm apart) versus separated as follows: greater diameter of DF (e.g., 10.5 ± 0.4 vs. 9.0 ± 0.4 mm), greater percentage of the DF wall with color Doppler signals of blood flow (40.2% ± 2.0% vs. 24.5% ± 1.9%), greater cross-sectional area of the CL (2.2 ± 0.1 vs. 1.8 ± 0.2 cm(2)), and greater percentage of the entire CL with blood flow signals (51.8% ± 1.2% vs. 42.5% ± 3.1%). Additional examples of positive coupling are (1) future DF on Day 0 (day of ovulation) is closer to the CL than the future largest subordinate and (2) diameter of growing follicles on Day 0 and the growth rate on Days 0 to 2 are greater for follicles that are adjacent than separated from the CL. An example of a negative intraovarian effect is decreasing diameter and loss of future DF status of a largest follicle when adjacent to a regressing CL. The impact of the continuity of ovarian angioarchitecture during the periovulatory follicular wave was exemplified in 17 of 18 waves by conversion of an ovary with only the preovulatory follicle to the postovulatory DF-CL pattern. Functional angiocoupling from commonality in angioarchitecture of the DF and adjacent CL would account for both the positive two-way coupling between DF and CL during the luteal phase and the negative effect of a regressing CL on an adjacent follicle during luteolysis. PMID:27056414

  3. 3D-2D image registration for target localization in spine surgery: investigation of similarity metrics providing robustness to content mismatch

    De Silva, T.; Uneri, A.; Ketcha, M. D.; Reaungamornrat, S.; Kleinszig, G.; Vogt, S.; Aygun, N.; Lo, S.-F.; Wolinsky, J.-P.; Siewerdsen, J. H.

    2016-04-01

    In image-guided spine surgery, robust three-dimensional to two-dimensional (3D-2D) registration of preoperative computed tomography (CT) and intraoperative radiographs can be challenged by the image content mismatch associated with the presence of surgical instrumentation and implants as well as soft-tissue resection or deformation. This work investigates image similarity metrics in 3D-2D registration offering improved robustness against mismatch, thereby improving performance and reducing or eliminating the need for manual masking. The performance of four gradient-based image similarity metrics (gradient information (GI), gradient correlation (GC), gradient information with linear scaling (GS), and gradient orientation (GO)) with a multi-start optimization strategy was evaluated in an institutional review board-approved retrospective clinical study using 51 preoperative CT images and 115 intraoperative mobile radiographs. Registrations were tested with and without polygonal masks as a function of the number of multistarts employed during optimization. Registration accuracy was evaluated in terms of the projection distance error (PDE) and assessment of failure modes (PDE  >  30 mm) that could impede reliable vertebral level localization. With manual polygonal masking and 200 multistarts, the GC and GO metrics exhibited robust performance with 0% gross failures and median PDE  14% however, GO maintained robustness with a 0% gross failure rate. Overall, the GI, GC, and GS metrics were susceptible to registration errors associated with content mismatch, but GO provided robust registration (median PDE  =  5.5 mm, 2.6 mm IQR) without manual masking and with an improved runtime (29.3 s). The GO metric improved the registration accuracy and robustness in the presence of strong image content mismatch. This capability could offer valuable assistance and decision support in spine level localization in a manner consistent with clinical workflow.

  4. Interaction between two adjacent grounded sources in frequency domain semi-airborne electromagnetic survey

    Zhou, Haigen; Lin, Jun; Liu, Changsheng; Kang, Lili; Li, Gang; Zeng, Xinsen

    2016-03-01

    Multi-source and multi-frequency emission method can make full use of the valuable and short flight time in frequency domain semi-airborne electromagnetic (FSAEM) exploration, which has potential to investigate the deep earth structure in complex terrain region. Because several sources are adjacent in multi-source emission method, the interaction of different sources should be considered carefully. An equivalent circuit model of dual-source is established in this paper to assess the interaction between two individual sources, where the parameters are given with the typical values based on the practical instrument system and its application. By simulating the output current of two sources in different cases, the influence from the adjacent source is observed clearly. The current waveforms show that the mutual resistance causes the fluctuation and drift in another source and that the mutual inductance causes transient peaks. A field test with dual-source was conducted to certify the existence of interaction between adjacent sources. The simulation of output current also shows that current errors at low frequency are mainly caused by the mutual resistance while those at high frequency are mainly due to the mutual inductance. Increasing the distance between neighboring sources is a proposed measure to reduce the emission signal errors with designed ones. The feasible distance is discussed in the end. This study gives a useful guidance to lay multi sources to meet the requirement of measurement accuracy in FSAEM survey.

  5. Historical records of eutrophication in Changjiang (Yangtze River estuary and its adjacent East China Sea

    F. Cheng

    2012-06-01

    Full Text Available Two sediment cores from the Changjiang (Yangtze River estuary and its adjacent East China Sea were collected and studied for eutrophication history using paleoecological records of environmental changes over the last century. A multiproxy approach by using biological and geochemical analyses revealed changes in diatom assemblages, total organic carbon (TOC, total nitrogen (TN and biogenic silica (BSi and give an indication of nutrient in status and trends in Changjiang River estuary and its adjacent East China Sea. The diatom assemblages in the two cores generally increased gradually from the 1970s, and accelerated from the 1990s until now, reflecting the increased eutrophication and causing large algae blooms/red tides. The TOC, TN and BSi showing the similar trends, supported the interpretation of the eutrophication process indicated by diatom analyses. The two cores were located in different sea areas of the East China Sea, and we discuss their relative changes based on their environment characteristics. We also discuss the potential effect of anthropogenic influences and ongoing projects on eutrophication in the Changjiang River and its adjacent East China Sea.

  6. Evaluating the Competency Mismatch between Master of Engineering Graduates and Industry Needs in China

    Peng, Lijun; Zhang, Shulin; Gu, Jibao

    2016-01-01

    This study investigates the mismatch between the educational attainment of a graduate with a Master of Engineering (MEng) degree and the industry needs in China. A competency list for MEng graduates from the perspective of industry needs was constructed. And a survey was conducted among MEng graduate students, alumni, and employers to assess the…

  7. A mismatch in the human realism of face and voice produces an uncanny valley

    Mitchell, Wade J; Szerszen, Kevin A; Lu, Amy Shirong; Schermerhorn, Paul W; Scheutz, Matthias; MacDorman, Karl F.

    2011-01-01

    The uncanny valley has become synonymous with the uneasy feeling of viewing an animated character or robot that looks imperfectly human. Although previous uncanny valley experiments have focused on relations among a character's visual elements, the current experiment examines whether a mismatch in the human realism of a character's face and voice causes it to be evaluated as eerie. The results support this hypothesis.

  8. High performance, high bandgap, lattice-mismatched, GaInP solar cells

    Wanlass, Mark W.; Carapella, Jeffrey J.; Steiner, Myles A.

    2014-07-08

    High performance, high bandgap, lattice-mismatched, photovoltaic cells (10), both transparent and non-transparent to sub-bandgap light, are provided as devices for use alone or in combination with other cells in split spectrum apparatus or other applications.

  9. Mismatch responses in the awake rat: evidence from epidural recordings of auditory cortical fields.

    Fabienne Jung

    Full Text Available Detecting sudden environmental changes is crucial for the survival of humans and animals. In the human auditory system the mismatch negativity (MMN, a component of auditory evoked potentials (AEPs, reflects the violation of predictable stimulus regularities, established by the previous auditory sequence. Given the considerable potentiality of the MMN for clinical applications, establishing valid animal models that allow for detailed investigation of its neurophysiological mechanisms is important. Rodent studies, so far almost exclusively under anesthesia, have not provided decisive evidence whether an MMN analogue exists in rats. This may be due to several factors, including the effect of anesthesia. We therefore used epidural recordings in awake black hooded rats, from two auditory cortical areas in both hemispheres, and with bandpass filtered noise stimuli that were optimized in frequency and duration for eliciting MMN in rats. Using a classical oddball paradigm with frequency deviants, we detected mismatch responses at all four electrodes in primary and secondary auditory cortex, with morphological and functional properties similar to those known in humans, i.e., large amplitude biphasic differences that increased in amplitude with decreasing deviant probability. These mismatch responses significantly diminished in a control condition that removed the predictive context while controlling for presentation rate of the deviants. While our present study does not allow for disambiguating precisely the relative contribution of adaptation and prediction error processing to the observed mismatch responses, it demonstrates that MMN-like potentials can be obtained in awake and unrestrained rats.

  10. Structural effects of internucleotide linkage modification at mismatch site in DNA/RNA duplex: Raman study

    Kříž, M.; Němeček, D.; Turpin, P. Y.; Rosenberg, Ivan; Štěpánek, J.

    2008-01-01

    Roč. 48, č. 1 (2008), s. 148-152. ISSN 0924-2031 R&D Projects: GA ČR GA203/05/0827 Institutional research plan: CEZ:AV0Z40550506 Keywords : nucleic acid * mismatch * hybrid duplex * Raman spectroscopy Subject RIV: CC - Organic Chemistry Impact factor: 1.810, year: 2008

  11. Influence of donor–recipient sex mismatch on long-term survival of pancreatic grafts

    Li, Zhiwei; Mei, Shengmin; Xiang, Jie; Zhou, Jie; Zhang, Qijun; Yan, Sheng; Zhou, Lin; Hu, Zhenhua; Zheng, Shusen

    2016-01-01

    To assess the role of sex mismatch on graft survival after pancreas transplantation. We evaluated 24,195 pancreas-transplant recipients reported in the Scientific Registry of Transplant Recipients over a 25-year period. Pancreatic graft survival (PGS) was analyzed according to donor–recipient sex pairing using Kaplan–Meier estimations. Hazard ratios were estimated using Cox proportional hazard models. A total of 14,187 male and 10,008 female recipients were included in final analyses. Mean follow-up was 8.3 ± 5.7 years. In multivariate analyses, neither recipient sex nor donor sex was associated with pancreatic graft failure (PGF), but donor–recipient sex mismatch (regardless of recipient sex) was an independent predictor of PGS (HR, 1.09; 95% CI, 1.04–1.14; p donor factors eliminated the association between F → M sex mismatch and PGF (HR, 1.02; 95% CI, 0.93–1.10; p = 0.752), but not M → F (1.09; 1.02–1.17; 0.020). Stratified analyses suggested that the negative effect of donor–recipient sex mismatch could be neutralized in older patients. These findings suggest that donor–recipient sex pairing should be taken into consideration in organ-allocation strategies. PMID:27403718

  12. Mismatch repair defective breast cancer in the hereditary nonpolyposis colorectal cancer syndrome

    Jensen, Uffe Birk; Sunde, Lone; Timshel, Susanne; Halvarsson, Britta; Nissen, Anja; Bernstein, Inge; Nilbert, Mef

    2010-01-01

    Whether or not breast cancer can be a feature of the hereditary nonpolyposis colorectal cancer (HNPCC) syndrome has been debated. In order to clarify if defective mismatch repair (MMR) may indeed play a role in breast cancer, we used the Danish HNPCC register to identify all breast cancers that o...

  13. The Extent of Skills Mismatch among Childhood Education Graduates of Princess Alia University College

    Nashash, Hyam M.

    2015-01-01

    This study is aimed at investigating the extent of skill mismatch between the skills the childhood education graduates at Al-Balqa Applied University--Princess Alia University College acquired during their studies and those demanded in the labor market. The descriptive survey design was adopted and the purposive sampling technique was employed to…

  14. Educational Mismatches and Earnings: Extensions of Occupational Mobility Theory and Evidence of Human Capital Depreciation

    Rubb, Stephen

    2006-01-01

    Using a human capital theory framework, this study examines the impact of educational mismatches on earnings and occupational mobility. Occupational mobility theory suggests that overeducated workers observe greater upward occupational mobility and undereducated workers observe lower upward occupational mobility. By extension, this leads to…

  15. The match-mismatch model of emotion processing styles and emotion regulation strategies in fibromyalgia.

    Geenen, R.; Ooijen-van der Linden, L. van; Lumley, M.A.; Bijlsma, J.W.J.; Middendorp, H. van

    2012-01-01

    OBJECTIVE: Individuals differ in their style of processing emotions (e.g., experiencing affects intensely or being alexithymic) and their strategy of regulating emotions (e.g., expressing or reappraising). A match-mismatch model of emotion processing styles and emotion regulation strategies is propo

  16. Localization by interaural time difference (ITD): Effects of interaural frequency mismatch

    A commonly accepted physiological model for lateralization of low-frequency sounds by interaural time delay (ITD) stipulates that binaural comparison neurons receive input from frequency-matched channels from each ear. Here, the effects of hypothetical interaural frequency mismatches on this model are reported. For this study, the cat close-quote s auditory system peripheral to the binaural comparison neurons was represented by a neurophysiologically derived model, and binaural comparison neurons were represented by cross-correlators. The results of the study indicate that, for binaural comparison neurons receiving input from one cochlear channel from each ear, interaural CF mismatches may serve to either augment or diminish the effective difference in ipsilateral and contralateral axonal time delays from the periphery to the binaural comparison neuron. The magnitude of this increase or decrease in the effective time delay difference can be up to 400 μs for CF mismatches of 0.2 octaves or less for binaural neurons with CFs between 250 Hz and 2.5 kHz. For binaural comparison neurons with nominal CFs near 500 Hz, the 25-μs effective time delay difference caused by a 0.012-octave CF mismatch is equal to the ITD previously shown to be behaviorally sufficient for the cat to lateralize a low-frequency sound source. copyright 1999 Acoustical Society of America.

  17. Regional Differences in the Listener's Phonemic Inventory Affect Semantic Processing: A Mismatch Negativity (MMN) Study

    Brunelliere, Angele; Dufour, Sophie; Nguyen, Noel

    2011-01-01

    Using the mismatch negativity (MMN) response, we examined how Standard French and Southern French speakers access the meaning of words ending in /e/ or /[epsilon]/ vowels which are contrastive in Standard French but not in Southern French. In Standard French speakers, there was a significant difference in the amplitude of the brain response after…

  18. Cernunnos/XLF promotes the ligation of mismatched and noncohesive DNA ends.

    Tsai, Chun J; Kim, Sunny A; Chu, Gilbert

    2007-05-01

    Nonhomologous end-joining (NHEJ) repairs DNA double-strand breaks created by ionizing radiation or V(D)J recombination of the immunoglobulin genes. The breaks often leave mismatched or nonligatable ends, and NHEJ must repair the breaks with high efficiency and minimal nucleotide loss. Here, the NHEJ proteins Ku, DNA-dependent protein kinase catalytic subunit, XRCC4/Ligase IV, and Cernunnos/XRCC4-like factor joined mismatched and noncohesive DNA ends in the absence of processing factors. Depending on the mismatch, Cernunnos stimulated joining 8- to 150-fold. For substrates with a blunt end and a 3' overhanging end, Ku, XRCC4/Ligase IV, and Cernunnos ligated the 3' overhanging hydroxyl group to the 5' phosphate of the blunt end, leaving the other strand unjoined. This activity provides a mechanism for retaining 3' overhang sequences, as observed during V(D)J recombination in vivo. Thus, Cernunnos/XRCC4-like factor promotes a mismatched end (MEnd) DNA ligase activity to facilitate joining and to preserve DNA sequence. Furthermore, MEnd ligase activity may have applications in recombinant DNA technology. PMID:17470781

  19. Quasi-synchronization of delayed chaotic systems with parameters mismatch and stochastic perturbation

    Liu, Chao; Li, Chuandong; Li, Chaojie

    2011-10-01

    This paper studies synchronization behavior of coupled time-delay chaotic systems for the case that parameter mismatch and stochastic perturbation occur in the response system. We establish quasi-synchronization criterion by analyzing stochastic stability of synchronization error system. A numerical example is also presented to show the effectiveness of theoretical results.

  20. Job Supply and Demand for University Graduates in Spain: A (Relative) Mismatch Perspective

    Parellada, Marti; Duch, Nestor; Alvarez, Montserrat

    2009-01-01

    This article provides an analysis of job supply by Spanish firms and the demand for work, and the mismatch that occurs between these two variables. Data are taken for the year 2006, with particular attention to jobs offered by firms that require people with university degrees or other higher education qualifications. Demand and supply are broken…

  1. The role of the bacterial mismatch repair system in SOS-induced mutagenesis: a theoretical background

    A theoretical study is performed of the possible role of the methyl-directed mismatch repair system in the ultraviolet-induced mutagenesis of Escherichia coli bacterial cells. For this purpose, a mathematical model of the bacterial mismatch repair system is developed. Within this model, the key pathways of this type of repair are simulated on the basis of modern experimental data related to its mechanisms. Here we have modelled in detail five main pathways of DNA misincorporation removal with different DNA exonucleases. Using our calculations, we have tested the hypothesis that the bacterial mismatch repair system is responsible for the removal of the nucleotides misincorporated by DNA polymerase V (the UmuD'2C complex) during ultraviolet-induced SOS response. For the theoretical analysis of the mutation frequency, we have combined the proposed mathematical approach with the model of SOS-induced mutagenesis in the E.coli bacterial cell developed earlier. Our calculations support the hypothesis that methyl-directed mismatch repair influences the mutagenic effect of ultraviolet radiation

  2. A rare case mimicking positron emission tomography/computed tomography mismatch: Hepatic subcapsular hematoma

    Subcapsular collections of bile, air or blood in the liver have been described following transhepatic procedures due to the leakage of bile and blood from the percutaneous puncture at the surface of the liver. Herein we presented the subcapsular collection led to a mismatch between functional and anatomical boundaries of the liver

  3. Free-energy formula for emittance-growth estimation in intense mismatched beams

    Osaki, Kazuya; Okamoto, Hiromi

    2015-09-01

    We construct a theoretical model that allows a quick estimate of emittance growth in an intense charged-particle beam initially mismatched to an external linear focusing potential. The present theory is a natural generalization of Reiser's free-energy model for coasting round beams in a uniform focusing channel. The free energy generated by a spatial mismatch, i.e. a discrepancy between the ideal beam size and an actual beam size, is calculated for an ellipsoidal bunch with an arbitrary aspect ratio. Following Reiser's prescription, we assume that the excess free energy is converted into root-mean-squared emittance growth. Multi-particle simulations are performed for comparison with theoretical predictions, which indicates that an initially mismatched bunch eventually settles into a sort of thermally anisotropic state when the mismatch is large. It is shown that the free-energy formula can explain simulation results over a wide range of parameters if the degree of the temperature anisotropy in the final state is properly incorporated into the theory.

  4. Interaural Place-Mismatch Estimation With Two-Formant Vowels in Unilateral Cochlear- Implant Users

    Guérit, François; Santurette, Sébastien; Chalupper, Josef;

    stimulation, with listeners showing either basal or apical shifts, or generally-poor vowel discrimination. Conclusion The two-formant-vowel method is a fast and clinic-friendly candidate to derive interaural place mismatches from a simple vowel-recognition task. However, it remains unclear whether...

  5. The Impact of Educational Mismatch on Firm Productivity: Evidence from Linked Panel Data

    Kampelmann, Stephan; Rycx, Francois

    2012-01-01

    We provide first evidence regarding the direct impact of educational mismatch on firm productivity. To do so, we rely on representative linked employer-employee panel data for Belgium covering the period 1999-2006. Controlling for simultaneity issues, time-invariant unobserved workplace characteristics, cohort effects and dynamics in the…

  6. Influence of donor-recipient sex mismatch on long-term survival of pancreatic grafts.

    Li, Zhiwei; Mei, Shengmin; Xiang, Jie; Zhou, Jie; Zhang, Qijun; Yan, Sheng; Zhou, Lin; Hu, Zhenhua; Zheng, Shusen

    2016-01-01

    To assess the role of sex mismatch on graft survival after pancreas transplantation. We evaluated 24,195 pancreas-transplant recipients reported in the Scientific Registry of Transplant Recipients over a 25-year period. Pancreatic graft survival (PGS) was analyzed according to donor-recipient sex pairing using Kaplan-Meier estimations. Hazard ratios were estimated using Cox proportional hazard models. A total of 14,187 male and 10,008 female recipients were included in final analyses. Mean follow-up was 8.3 ± 5.7 years. In multivariate analyses, neither recipient sex nor donor sex was associated with pancreatic graft failure (PGF), but donor-recipient sex mismatch (regardless of recipient sex) was an independent predictor of PGS (HR, 1.09; 95% CI, 1.04-1.14; p donor factors eliminated the association between F → M sex mismatch and PGF (HR, 1.02; 95% CI, 0.93-1.10; p = 0.752), but not M → F (1.09; 1.02-1.17; 0.020). Stratified analyses suggested that the negative effect of donor-recipient sex mismatch could be neutralized in older patients. These findings suggest that donor-recipient sex pairing should be taken into consideration in organ-allocation strategies. PMID:27403718

  7. Visual Task Demands and the Auditory Mismatch Negativity: An Empirical Study and a Meta-Analysis

    Wiens, Stefan; Szychowska, Malina; Nilsson, Mats E.

    2016-01-01

    Because the auditory system is particularly useful in monitoring the environment, previous research has examined whether task-irrelevant, auditory distracters are processed even if subjects focus their attention on visual stimuli. This research suggests that attentionally demanding visual tasks decrease the auditory mismatch negativity (MMN) to simultaneously presented auditory distractors. Because a recent behavioral study found that high visual perceptual load decreased detection sensitivity of simultaneous tones, we used a similar task (n = 28) to determine if high visual perceptual load would reduce the auditory MMN. Results suggested that perceptual load did not decrease the MMN. At face value, these nonsignificant findings may suggest that effects of perceptual load on the MMN are smaller than those of other demanding visual tasks. If so, effect sizes should differ systematically between the present and previous studies. We conducted a selective meta-analysis of published studies in which the MMN was derived from the EEG, the visual task demands were continuous and varied between high and low within the same task, and the task-irrelevant tones were presented in a typical oddball paradigm simultaneously with the visual stimuli. Because the meta-analysis suggested that the present (null) findings did not differ systematically from previous findings, the available evidence was combined. Results of this meta-analysis confirmed that demanding visual tasks reduce the MMN to auditory distracters. However, because the meta-analysis was based on small studies and because of the risk for publication biases, future studies should be preregistered with large samples (n > 150) to provide confirmatory evidence for the results of the present meta-analysis. These future studies should also use control conditions that reduce confounding effects of neural adaptation, and use load manipulations that are defined independently from their effects on the MMN. PMID:26741815

  8. The Role of Education Pathways in the Relationship between Job Mismatch, Wages and Job Satisfaction: A Panel Estimation Approach

    Mavromaras, Kostas; Sloane, Peter; Wei, Zhang

    2012-01-01

    This paper examines the outcome of over-skilling and over-education on wages and job satisfaction of full-time employees in Australia between 2001 and 2008. We employ a random effects probit model with Mundlak corrections. We find differences by type of mismatch, education pathway, and gender. We categorise reported mismatches as genuine…

  9. How metamer mismatching decreases as the number of colour mechanisms increases with implications for colour and lightness constancy.

    Logvinenko, Alexander D; Funt, Brian; Godau, Christoph

    2015-08-01

    Metamer mismatching has been previously found to impose serious limitations on colour constancy. The extent of metamer mismatching is shown here to be considerably smaller for trichromats than for dichromats, and maximal for monochromats. The implications for achromatic colour perception are discussed. PMID:26054251

  10. Visual mismatch negativity (vMMN): A review and meta-analysis of studies in psychiatric and neurological disorders.

    Kremláček, Jan; Kreegipuu, Kairi; Tales, Andrea; Astikainen, Piia; Põldver, Nele; Näätänen, Risto; Stefanics, Gábor

    2016-07-01

    The visual mismatch negativity (vMMN) response is an event-related potential (ERP) component, which is automatically elicited by events that violate predictions based on prior events. VMMN experiments use visual stimulus repetition to induce predictions, and vMMN is obtained by subtracting the response to rare unpredicted stimuli from those to frequent stimuli. One increasingly popular interpretation of the mismatch response postulates that vMMN, similar to its auditory counterpart (aMMN), represents a prediction error response generated by cortical mechanisms forming probabilistic representations of sensory signals. Here we discuss the physiological and theoretical basis of vMMN and review thirty-three studies from the emerging field of its clinical applications, presenting a meta-analysis of findings in schizophrenia, mood disorders, substance abuse, neurodegenerative disorders, developmental disorders, deafness, panic disorder and hypertension. Furthermore, we include reports on aging and maturation as they bear upon many clinically relevant conditions. Surveying the literature we found that vMMN is altered in several clinical populations which is in line with aMMN findings. An important potential advantage of vMMN however is that it allows the investigation of deficits in predictive processing in cognitive domains which rely primarily on visual information; a principal sensory modality and thus of vital importance in environmental information processing and response, and a modality which arguably may be more sensitive to some pathological changes. However, due to the relative infancy of research in vMMN compared to aMMN in clinical populations its potential for clinical application is not yet fully appreciated. The aim of this review and meta-analysis therefore is to present, in a detailed systematic manner, the findings from clinically-based vMMN studies, to discuss their potential impact and application, to raise awareness of this measure and to improve our

  11. Historical Earthquakes in the Yellow Sea and Its Adjacent Area

    Wu Ge; Wang Andong; Wu Di

    2005-01-01

    As a result of sorting out, estimating and cataloging of historical earthquakes, from the year of 2 A.D. to Aug., 1949, we found that there were 2187 earthquakes with M≥3.0 in the area of the Yellow Sea and its adjacent area. Among the earthquakes, the number of earthquakes with M ≥ 5.0 is 209, and at least 43 of the earthquakes caused serious losses, 20 of the earthquakes caused human causalities. It is demonstrated that there were 3 areas of historical earthquake concentration and the earthquake activity was higher in the 16th century and the first half if the 20th century.

  12. Experimental Tape Casting of Adjacently Graded Materials for Magnetic Refrigeration

    Bulatova, Regina

    graded thin films. The motivation to create such adjacently segmented structures stemmed from a search of material and a materials design alternative to the expensive rare earth element gadolinium which is widely used as a magnetic regenerator in the emerging magnetic refrigeration technology....... Conventional tape casting involves the preparation of specially formulated slurry, which is cast by a blade to a thin flat tape, then dried into flexible so-called green solid tape and can be subsequently sintered into a hard ceramic material. The principal difference and, at the same time, a challenge...

  13. The formation of adjacent triplex-duplex domainsin DNA.

    Nam, K. H.; Abhiraman, S; Wartell, R M

    1999-01-01

    The ability of single-stranded DNA oligomers to form adjacent triplex and duplex domains with two DNA structural motifs was examined. Helix-coil transition curves and a gel mobility shift assay were used to characterize the interaction of single-stranded oligomers 12-20 nt in length with a DNA hairpin and with a DNA duplex that has a dangling end. The 12 nt on the 5'-ends of the oligomers could form a triplex structure with the 12 bp stem of the hairpin or the duplex portion of the DNA with a...

  14. Adjacent Lumbar Disc Herniation after Lumbar Short Spinal Fusion

    Koshi Ninomiya; Koichi Iwatsuki; Yu-ichiro Ohnishi; Toshika Ohkawa; Toshiki Yoshimine

    2014-01-01

    A 70-year-old outpatient presented with a chief complaint of sudden left leg motor weakness and sensory disturbance. He had undergone L4/5 posterior interbody fusion with L3–5 posterior fusions for spondylolisthesis 3 years prior, and the screws were removed 1 year later. He has been followed up for 3 years, and there had been no adjacent segment problems before this presentation. Lumbar magnetic resonance imaging (MRI) showed a large L2/3 disc hernia descending to the L3/4 level. Compared to...

  15. Exophytic Atheroma Mimicking Papillary Fibroelastoma Adjacent to the Aortic Valve.

    Cho, Tomoki; Tokunaga, Shigehiko; Yasuda, Shota; Izubuchi, Ryo; Masuda, Munetaka

    2015-09-01

    Follow-up echocardiography in a 69-year-old man with alcoholic cardiomyopathy showed a mass above the aortic valve near the left coronary ostium. Transesophageal echocardiography and computed tomography suggested a papillary fibroelastoma with a high risk of embolism. At operation we found an exophytic atheroma adjacent to the left coronary artery orifice. The atheroma was removed, and the patient made an uneventful recovery. We describe this very rare case of an exophytic atheroma mimicking a papillary fibroelastoma situated at the left coronary orifice. PMID:26354633

  16. An ElGamal Encryption Scheme of Adjacency Matrix and

    B. Ravi Kumar

    2015-10-01

    Full Text Available  Cryptography is the combination of Mathematics and Computer science. Cryptography is used for encryption and decryption of data using mathematics. Cryptography transit the information in an illegible manner such that only intended recipient will be able to decrypt the information. In the recent years, researchers developed several new encryption methods. Among such ElGamal encryption is the one laid a concede platform for the researchers in Cryptography. Ever science several mathematical models were applied for encryption/decryption. In this paper, we introduced an ElGamal encryption, which uses points on the elliptic curve, and finite state machines and adjacency matrix.

  17. Abnormal auditory mismatch response in tinnitus sufferers with high-frequency hearing loss is associated with subjective distress level

    Berg Patrick

    2004-03-01

    Full Text Available Abstract Background Tinnitus is an auditory sensation frequently following hearing loss. After cochlear injury, deafferented neurons become sensitive to neighbouring intact edge-frequencies, guiding an enhanced central representation of these frequencies. As psychoacoustical data 123 indicate enhanced frequency discrimination ability for edge-frequencies that may be related to a reorganization within the auditory cortex, the aim of the present study was twofold: 1 to search for abnormal auditory mismatch responses in tinnitus sufferers and 2 relate these to subjective indicators of tinnitus. Results Using EEG-mismatch negativity, we demonstrate abnormalities (N = 15 in tinnitus sufferers that are specific to frequencies located at the audiometrically normal lesion-edge as compared to normal hearing controls (N = 15. Groups also differed with respect to the cortical locations of mismatch responsiveness. Sources in the 90–135 ms latency window were generated in more anterior brain regions in the tinnitus group. Both measures of abnormality correlated with emotional-cognitive distress related to tinnitus (r ~ .76. While these two physiological variables were uncorrelated in the control group, they were correlated in the tinnitus group (r = .72. Concerning relationships with parameters of hearing loss (depth and slope, slope turned out to be an important variable. Generally, the steeper the hearing loss is the less distress related to tinnitus was reported. The associations between slope and the relevant neurophysiological variables are in agreement with this finding. Conclusions The present study is the first to show near-to-complete separation of tinnitus sufferers from a normal hearing control group based on neurophysiological variables. The finding of lesion-edge specific effects and associations with slope of hearing loss corroborates the assumption that hearing loss is the basis for tinnitus development. It is likely that some central

  18. Charter School Autonomy: The Mismatch between Theory and Practice

    Finnigan, Kara S.

    2007-01-01

    In theory, the charter school concept is based on a trade-off or exchange: greater autonomy for increased accountability. Although charter schools have been operating for more than 10 years, little is known about charter school autonomy in practice. This mixed-methods study used survey and case study data to examine the degree of autonomy of…

  19. Adjacent stage impurity ratio in rare earth countercurrent extraction process

    CHENG Fuxiang; WU Sheng; LIAO Chunsheng; YAN Chunhua

    2013-01-01

    Impurity components decrease stage by stage in a cascade of rare earth (RE) extraction separation,and adjacent stage impurity ratio (ASIR) which is defined as the ratio of an impurity's contents in the aqueous/organic phase of two adjacent stages can be used to evaluate the capacity of impurity removal for the two stages.On the basis of extraction equilibrium and mass balance,the ASIR in a two-component extraction separation was deducted and its simplified expressions were given for different process sections according to reasonable assumptions.The calculation simulation was then carried out to obtain the ASIR distribution in the cascade.The results showed that in both the extraction and scrubbing sections the ASIR principally increased with the decrease of the molar proportion of the impurity but along with a flat appearing in the purification zone located in the middle of the cascade.The ASIR intuitively exhibits the nmning status of RE extraction separation and purification,which could provide a theoretic guide for investigating the influence factors of RE extraction separation process in practical industry.

  20. Adjacent segment disease in degenerative pathologies with posterior instrumentation

    Ana Guadalupe Ramírez Olvera

    2015-03-01

    Full Text Available OBJECTIVE: To establish the real incidence of adjacent segment disease after fusion, and to identify the levels and predisposing factors for the pathology, as well as the functional results. METHODS: a retrospective case series study with level of evidence IIB, in a sample of 179 patients diagnosed with stenosis of the lumbar spine, spondylolisthesis and degenerative scoliosis, submitted to surgery in the period 2005 to December 2013, with posterior instrumentation and posterolateral fusion, with follow-up from 2007 until May 2014, in which the symptomology and radiographic findings were evaluated, to establish the diagnosis and treatment. RESULTS: the study included 179 patients diagnosed with stenosis of the lumbar spine (n=116, isthmic and degenerative spondylolisthesis (n=50 and degenerative scoliosis (n=13; during the study, 20 cases of adjacent level segment were identified, 80% of which were treated surgically with extension of the instrumentation, while 20% were treated conservatively with NSAIDs and therapeutic blocks. CONCLUSION: An incidence of 11% was found, with an average of 3.25 years in diagnosis and treatment, a prevalence of females and diagnosis of stenosis of the lumbar canal on posterior instrumentation, a predominance of levels L4-L5; 80% were treated with extension of the instrumentation. The complications were persistent radiculopathy, infection of the surgical wound, and one death due to causes not related to the lumbar pathology.

  1. Absorbed dose distribution analyses in irradiation with adjacent fields

    Because the special irradiation technique with adjacent fields is the most used in the case of medulloblastoma treatment, we consider very important to specify some general information about medulloblastoma. This malignant disease has a large incidence in children with age between 5-7 years. This tumor usually originates in the cerebellum and is referred to as primitive undifferentiated tumor. It may spread contiguously to the cerebellar peduncle, floor of the fourth ventricle, into the cervical spine. In addition, it may spread via the cerebrospinal fluid intracranially and/or to the spinal cord. For this purpose it is necessary to perform a treatment technique with cranial tangential fields combined with adjacent fields for the entire spinal cord to achieve a perfect coverage of the zones with malignant cells. The treatment in this case is an association between surgery-radio-chemotherapy, where the radiotherapy has a very important roll and a curative purpose. This is due to the fact that the migration of malignant cells in the body can't be controlled by surgery. Because of this special irradiation technique used in medulloblastoma treatment, we chase to describe in this paper this complex type of irradiation where the implications of the beams divergence in doses distribution are essentials

  2. Helix geometry, hydration, and G.A mismatch in a B-DNA decamer.

    Privé, G G; Heinemann, U; Chandrasegaran, S; Kan, L S; Kopka, M L; Dickerson, R E

    1987-10-23

    The DNA double helix is not a regular, featureless barberpole molecule. Different base sequences have their own special signature, in the way that they influence groove width, helical twist, bending, and mechanical rigidity or resistance to bending. These special features probably help other molecules such as repressors to read and recognize one base sequence in preference to another. Single crystal x-ray structure analysis is beginning to show us the various structures possible in the B-DNA family. The DNA decamer C-C-A-A-G-A-T-T-G-G appears to be a better model for mixed-sequence B-DNA than was the earlier C-G-C-G-A-A-T-T-C-G-C-G, which is more akin to regions of poly(dA).poly(dT). The G.A mismatch base pairs at the center of the decamer are in the anti-anti conformation about their bonds from base to sugar, in agreement with nuclear magnetic resonance evidence on this and other sequences, and in contrast to the anti-syn geometry reported for G.A pairs in C-G-C-G-A-A-T-T-A-G-C-G. The ordered spine of hydration seen earlier in the narrow-grooved dodecamer has its counterpart, in this wide-grooved decamer, in two strings of water molecules lining the walls of the minor groove, bridging from purine N3 or pyrimidine O2, to the following sugar O4'. The same strings of hydration are present in the phosphorothioate analog of G-C-G-C-G-C. Unlike the spine, which is broken up by the intrusion of amine groups at guanines, these water strings are found in general, mixed-sequence DNA because they can pass by unimpeded to either side of a guanine N2 amine. The spine and strings are perceived as two extremes of a general pattern of hydration of the minor groove, which probably is the dominant factor in making B-DNA the preferred form at high hydration. PMID:3310237

  3. Research on algorithm of positioning of the eyes based on adjacent frame YCbCr domain difference model%基于YCbCr颜色空间相邻帧差建模的眼睛定位算法研究

    梁万元; 种银保; 赵安; 向逾

    2014-01-01

    Objective:To research an eye precise localization method based on eye movement detection model, which is used to improve the speed of the localization method in traditional eye precise localization methods. Methods: Firstly, this paper improves temporal differencing with the skin color segmentation model and builds a model to detect the movement of eyes meanwhile. It’s easy to get the movement range of eyes in adjacency photographs using the eye movement detection model in this paper and then we can get the rectangular region consisted by the eyes in the photograph which is to be detected. Finally, using the morphology method can help to get the accuracy region of eyes. Results:The experimental result shows that the speed of localization method in this paper increase to 14fps and the accuracy is almost the same as the traditional eye localization methods. Conclusion: The experiment demonstrates the eye precise localization in this paper realizes a higher speed and ensure the accuracy at the same time.%目的:研究一种基于YCbCr相邻帧差法检测头部运动及眼睛位移、定位眼睛位置的算法,使之能在基于眼睛状态的疲劳驾驶监测中真正发挥作用。方法:利用YCbCr颜色空间改进相邻帧差法,并依据相邻帧差法对运动物体检测的原理,建立眼睛位移检测的模型。运用该模型检测出后一帧相对于前一帧的眼睛位移量,参照前一帧眼睛所在的矩形区域,框选出后一帧图像中眼睛的候选区域,再利用形态学方法处理,最终得到后一帧图像眼睛的精确矩形区域。结果:本算法的眼睛定位处理速度为14帧/s,较传统眼睛定位算法的9帧/s提升了55%,能确保实时检测到人眨眼瞬间(0.2~0.4 s)的时间间隙,且正确率与传统眼睛定位算法相当。结论:本研究的眼睛定位算法大幅提高了眼睛检测算法的速度,能确保实时检测到人眨眼瞬间(0.2~0.4 s)的时间间隙,提

  4. Clinicopathologic factors identify sporadic mismatch repair-defective colon cancers

    Halvarsson, Britta; Anderson, Harald; Domanska, Katarina;

    2008-01-01

    were linked to MMR status based on immunostaining and BRAF mutation status.MMR defects were identified in 22.7% of the tumors, with 46 classified as sporadic. When the clinical parameters of age, sex, and proximal tumor location were combined with the morphologic features with the highest relative...... risks (RRs), eg, mucinous differentiation (RR, 9.0), tumor-infiltrating lymphocytes (RR, 7.5), absence of necrosis (RR, 7.5), and expanding growth pattern (RR, 5.0) into a 7-factor index, the presence of at least 4 features identified the MMR-defective tumors with 92.3% sensitivity and 75.3% specificity...

  5. The Consequences on Job Satisfaction of Job-Worker Educational and Skill Mismatches in the Spanish Labour Market: a Panel Analysis

    Lourdes Badillo Amador; Angel López Nicolás; Vila, Luis E.

    2008-01-01

    The effects of job-worker mismatches on job satisfaction are examined using the eight waves (1994-2001) of Spanish data taken from the European Community Household Panel (ECHP). The impacts of both educational and skill mismatches are estimated considering unobserved heterogeneity, state dependence and attrition bias. Dynamic analysis shows that skill mismatches emerge as a much better predictor of job satisfaction than educational mismatches as the effects of the latter are related to unobse...

  6. Loss of Mismatched HLA on the Leukemic Blasts of Patients With Relapsed Lymphoid Malignancies Following Bone Marrow Transplantation From Related Donors With HLA Class II Mismatches in the Graft Versus Host Direction.

    Hirabayashi, Koichi; Kurata, Takashi; Horiuchi, Kazuki; Saito, Shoji; Shigemura, Tomonari; Tanaka, Miyuki; Yanagisawa, Ryu; Matsuda, Kazuyuki; Sakashita, Kazuo; Koike, Kenichi; Nakazawa, Yozo

    2016-04-01

    Mechanisms of relapse of acute lymphoblastic leukemia (ALL) after human leukocyte antigen (HLA) class II mismatched hematopoietic stem cell transplantation (HSCT) remain unclear. We report two children with relapsed ALL after HSCT from related donors with HLA-DRB1 and -DQB1 mismatches in the graft versus host direction. One lost HLA-DRB1, DQB1, and DPB1 alleles, and the other lost one HLA haplotype of the leukemic blasts at relapse. HLA class II loss may be a triggering event for ALL relapse after partially HLA-mismatched-related HSCT. In addition, HLA typing of relapsed leukemic blasts could be vital in the selection of retransplant donors. PMID:26544669

  7. Analysis of blasting damage in adjacent mining excavations

    Nick Yugo

    2015-06-01

    Full Text Available Following a small-scale wedge failure at Yukon Zinc's Wolverine Mine in Yukon, Canada, a vibration monitoring program was added to the existing rockbolt pull testing regime. The failure in the 1150 drift occurred after numerous successive blasts in an adjacent tunnel had loosened friction bolts passing through an unmapped fault. Analysis of blasting vibration revealed that support integrity is not compromised unless there is a geological structure to act as a failure plane. The peak particle velocity (PPV rarely exceeded 250 mm/s with a frequency larger than 50 Hz. As expected, blasting more competent rock resulted in higher PPVs. In such cases, reducing the round length from 3.5 m to 2.0 m was an effective means of limiting potential rock mass and support damage.

  8. Acyclic 6-choosability of planar graphs without adjacent short cycles

    WANG WeiFan; ZHANG Ge; CHEN Min

    2014-01-01

    A proper vertex coloring of a graph G is acyclic if G contains no bicolored cycles.Given a list assignment L={L(v)|v∈V}of G,we say that G is acyclically L-colorable if there exists a proper acyclic coloringπof G such thatπ(v)∈L(v)for all v∈V.If G is acyclically L-colorable for any list assignment L with|L(v)|k for all v∈V(G),then G is acyclically k-choosable.In this paper,we prove that every planar graph G is acyclically 6-choosable if G does not contain 4-cycles adjacent to i-cycles for each i∈{3,4,5,6}.This improves the result by Wang and Chen(2009).

  9. Adjacent Lumbar Disc Herniation after Lumbar Short Spinal Fusion

    Koshi Ninomiya

    2014-01-01

    Full Text Available A 70-year-old outpatient presented with a chief complaint of sudden left leg motor weakness and sensory disturbance. He had undergone L4/5 posterior interbody fusion with L3–5 posterior fusions for spondylolisthesis 3 years prior, and the screws were removed 1 year later. He has been followed up for 3 years, and there had been no adjacent segment problems before this presentation. Lumbar magnetic resonance imaging (MRI showed a large L2/3 disc hernia descending to the L3/4 level. Compared to the initial MRI, this hernia occurred in an “intact” disc among multilevel severely degenerated discs. Right leg paresis and bladder dysfunction appeared a few days after admission. Microscopic lumbar disc herniotomy was performed. The right leg motor weakness improved just after the operation, but the moderate left leg motor weakness and difficulty in urination persisted.

  10. New adjacent vertebral body fracture after percutaneous vertebroplasty

    Percutaneous vertebroplasty is widely used as a treatment for painful osteoporotic compression fractures and malignant osteolytic tumors. Although the procedure appears to provide dramatic pain relief promote life quality but also with some complications. Additional adjacent vertebral body fracture are frequently reported after vertebroplasty but the cause relationship between the procedure and new-onset vertebral fracture remains unknown. New compression fractures following percutaneous vertebroplasty may not be a complication of the procedure itself, but rather as a part of the course of underlying pathology. The crucial point of discussion in this paper is whether vertebroplasty predisposes the development of additional vertebral fractures, at a rate higher than that seen in the absence of vertebroplasty, but no definitive answer to this question is yet to come. This review explores and attempts to comprehend the data both of supporting and refuting a relationship between vertebroplasty and the development of subsequent fractures. (authors)

  11. Laser ablation of human atherosclerotic plaque without adjacent tissue injury

    Grundfest, W. S.; Litvack, F.; Forrester, J. S.; Goldenberg, T.; Swan, H. J. C.

    1985-01-01

    Seventy samples of human cadaver atherosclerotic aorta were irradiated in vitro using a 308 nm xenon chloride excimer laser. Energy per pulse, pulse duration and frequency were varied. For comparison, 60 segments were also irradiated with an argon ion and an Nd:YAG laser operated in the continuous mode. Tissue was fixed in formalin, sectioned and examined microscopically. The Nd:YAG and argon ion-irradiated tissue exhibited a central crater with irregular edges and concentric zones of thermal and blast injury. In contrast, the excimer laser-irradiated tissue had narrow deep incisions with minimal or no thermal injury. These preliminary experiments indicate that the excimer laser vaporizes tissue in a manner different from that of the continuous wave Nd:YAG or argon ion laser. The sharp incision margins and minimal damage to adjacent normal tissue suggest that the excimer laser is more desirable for general surgical and intravascular uses than are the conventionally used medical lasers.

  12. Classical Hodgkin Lymphoma Arising Adjacent to a Breast Implant.

    Ryan, Ciara; Ged, Yasser; Quinn, Fiona; Walker, Jan; Kennedy, John; Gillham, Charles; Pittaluga, Stefania; McDermott, Ronan; Vandenberghe, Elisabeth; Grant, Cliona; Flavin, Richard

    2016-08-01

    Breast implant-associated lymphoma has recently gained wide recognition. Anaplastic large cell lymphoma (ALCL) is the most frequently diagnosed subtype in this setting but the spectrum is broadening. A 66-year-old woman developed swelling and itch around her saline implant 6 years after its insertion. Imaging revealed a fluid collection surrounding the implant with an adjacent mass. Microscopy showed sclerotic tissue punctuated by discrete cellular nodules comprising small lymphocytes, eosinophils and interspersed large atypical Hodgkin Reed-Sternberg (HRS)-like cells. The HRS-like cells stained positively for CD30 and CD15 by immunohistochemistry. Small T-lymphocytes formed rosettes around HRS-like cells. Appearances were consistent with classical Hodgkin lymphoma (HL). Multiplex polymerase chain reaction demonstrated no clonal rearrangements of immunoglobulin or T-cell receptor genes, however, a t(14;18)(q32;q21)BCL2-JH translocation involving the major breakpoint region of the bcl2 gene was present. Staging positron emission tomography-computed tomography scan revealed FDG-avid masses in the right axilla and pelvis. Subsequent pathological examination identified low-grade follicular lymphoma (FL) with a t(14;18) translocation at these sites. To our knowledge, this is the first case of HL arising adjacent to a breast implant. An awareness of this diagnosis is important as classical HL, with its prominent mixed inflammatory background, may be overlooked as a reactive process when histologically assessing capsulectomy specimens. It is also important in the differential diagnosis for implant-associated ALCL as both contain large atypical CD30-positive cells highlighting the need for full immunohistochemical and molecular workup in such cases. This case also adds to the large body of literature regarding the association between HL and FL. PMID:26888955

  13. Increased apoptosis in gastric mucosa adjacent to intestinal metaplasia

    van Grieken, N C T; Meijer, G A; zur Hausen, A; Meuwissen, S G M; Baak, J P A; Kuipers, E J

    2003-01-01

    Background: The biological processes involved in the development of gastric mucosal atrophy and intestinal metaplasia are still incompletely understood. Reports testing the hypothesis that apoptosis leads to atrophy have yielded conflicting results. The availability of new antibodies for the detection of apoptotic cells in tissue sections has facilitated the analysis of the role of apoptosis in the gastritis–atrophy–intestinal metaplasia sequence. Methods: Archival material from 40 gastric resection specimens with normal mucosa (n = 5), chronic active gastritis (n = 17), or intestinal metaplasia (n = 18) was studied. Immunohistochemistry was performed using antibodies directed against cleaved cytokeratin 18 and active caspase 3. Slides were scored on a 0–3 scale for the presence of apoptotic cells. Results: Normal gastric mucosa contained low numbers of apoptotic cells at the surface epithelium (mean score, 0.20). This number was significantly increased in cases with chronic gastritis (mean score, 1.06) and in those with intestinal metaplasia (mean score, 2.56). Within the intestinal metaplasia cases, 44 different foci of intestinal metaplasia were identified. In 39 of these 44 areas, concentrations of apoptotic cells were seen immediately adjacent to the foci of intestinal metaplasia, but not in the metaplastic epithelium itself. Conclusions: Apoptosis is uncommon in normal gastric mucosa. Chronic inflammation and intestinal metaplasia are associated with increased apoptosis, but occur mainly at the mucosal surface and not in the deeper layers. These findings do not support the concept that apoptosis underlies the loss of gastric glands and leads to atrophy, but the observed concentration of apoptotic epithelial cells adjacent to foci of intestinal metaplasia could be related to heterogeneity of epithelial damage, causing apoptosis, to which intestinal metaplasia is a response. PMID:12719456

  14. Ultrafast and octave-spanning optical nonlinearities from strongly phase-mismatched cascaded interactions

    Zhou, B. B.; Chong, A.; Wise, F. W.;

    2012-01-01

    Cascaded nonlinearities have attracted much interest, but ultrafast applications have been seriously hampered by the simultaneous requirements of being near phase matching and having ultrafast femtosecond response times. Here we show that in strongly phase-mismatched nonlinear frequency conversion...... crystals the pump pulse can experience a large and extremely broadband self-defocusing cascaded Kerrlike nonlinearity. The large cascaded nonlinearity is ensured through interaction with the largest quadratic tensor element in the crystal, and the strong phase mismatch ensures an ultrafast nonlinear......% efficiency, and upon further propagation an octave-spanning supercontinuum is observed. Such ultrafast cascading is expected to occur for a broad range of pump wavelengths spanning the near- and mid-IR using standard nonlinear crystals....

  15. Expression of DNA mismatch repair proteins in transformed non-Hodgkin's lymphoma: relationship to smoking

    Nandi, S; Yu, J; Reinert, Line;

    2006-01-01

    It has been hypothesized that defects in DNA-mismatch repair are associated with smoking in certain types of transformed non-Hodgkin lymphoma (NHL). We have analyzed biopsy samples from two indolent B-cell lymphomas, follicular lymphoma (FL) and chronic lymphocytic leukemia/small lymphocytic...... leukemia (CLL/SLL), that have transformed to diffuse-large B-cell lymphoma (DLBCL). We correlated the presence or absence of DNA-mismatch repair enzymes by immunostaining as well as the p53 status to smoking history. Of all patients (n = 30), 37% showed negative immunostaining of MLH1, 16% showed negative...... immunostaining of MSH2 and 63% had p53 mutations and/or protein expression. Eighteen out of 20 transformed follicular lymphomas and seven out of 10 CLL/SLL that have transformed to DLBCL (Richter's syndrome) were informative for smoking histories. We found that the relative risk of negative immunostaining for...

  16. Chaos synchronization in time-delayed systems with parameter mismatches and variable delay times

    We investigate synchronization between two undirectionally linearly coupled chaotic nonidentical time-delayed systems and show that parameter mismatches are of crucial importance to achieve synchronization. We establish that independent of the relation between the delay time in the coupled systems and the coupling delay time, only retarded synchronization with the coupling delay time is obtained. We show that with parameter mismatch or without it neither complete nor anticipating synchronization occurs. We derive existence and stability conditions for the retarded synchronization manifold. We demonstrate our approach using examples of the Ikeda and Mackey Glass models. Also for the first time we investigate chaos synchronization in time-delayed systems with variable delay time and find both existence and sufficient stability conditions for the retarded synchronization manifold with the coupling-delay lag time. (author)

  17. HLA-mismatched hematopoietic stem cell tranplantation for pediatric solid tumors

    Andrea Pession

    2011-06-01

    Full Text Available Even if the overall survival of children with cancer is significantly improved over these decades, the cure rate of high-risk pediatric solid tumors such as neuroblastoma, Ewing’s sarcoma family tumors or rhabdomiosarcoma remain challenging. Autologous hematopoietic stem cell transplantation (HSCT allows chemotherapy dose intensification beyond marrow tolerance and has become a fundamental tool in the multimodal therapeutical approach of these patients. Anyway this procedure does not allow to these children an eventfree survival approaching more than 50% at 5 years. New concepts of allogeneic HSCT and in particular HLA-mismatched HSCT for high risk solid tumors do not rely on escalation of chemo therapy intensity and tumor load reduction but rather on a graft-versus-tumor effect. We here report an experimental study design of HLA-mismatched HSCT for the treatment of pediatric solid tumors and the inherent preliminary results.

  18. Effects of carrier phase estimation on front-end IQ mismatch compensation in mode division multiplexed MIMO coherent receiver

    Chung, Hwan Seok; Chang, Sun Hyok; Kim, Kwangjoon; Lee, Jong Hyun

    2014-01-01

    The effects of carrier phase estimation on IQ mismatch compensation are investigated and compared for coherent MIMO optical receiver. We investigate the impacts of phase IQ mismatch on required optical signal-to-noise ratio (OSNR) for coherent MIMO detection. The impacts of number of modes and analog-to-digital converter (ADC) resolution are also evaluated. The results show that decision-directed carrier phase estimator could compensate significant amount of IQ mismatch without employing additional compensation technique, and relaxes requirement of ADC resolution.

  19. Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi.

    Yusuke Ohnishi

    Full Text Available Allele-specific gene silencing by RNA interference (RNAi is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi (ASP-RNAi, the design and assessment of small interfering RNA (siRNA duplexes conferring ASP-RNAi is vital; however, it is also difficult. In a previous study, we developed an assay system to assess ASP-RNAi with mutant and wild-type reporter alleles encoding the Photinus and Renilla luciferase genes. In line with experiments using the system, we realized that it is necessary and important to enhance allele discrimination between mutant and corresponding wild-type alleles. Here, we describe the improvement of ASP-RNAi against mutant alleles carrying single nucleotide variations by introducing base substitutions into siRNA sequences, where original variations are present in the central position. Artificially mismatched siRNAs or short-hairpin RNAs (shRNAs against mutant alleles of the human Prion Protein (PRNP gene, which appear to be associated with susceptibility to prion diseases, were examined using this assessment system. The data indicates that introduction of a one-base mismatch into the siRNAs and shRNAs was able to enhance discrimination between the mutant and wild-type alleles. Interestingly, the introduced mismatches that conferred marked improvement in ASP-RNAi, appeared to be largely present in the guide siRNA elements, corresponding to the 'seed region' of microRNAs. Due to the essential role of the 'seed region' of microRNAs in their association with target RNAs, it is conceivable that disruption of the base-pairing interactions in the corresponding seed region, as well as the central position (involved in cleavage of target RNAs, of guide siRNA elements could influence allele discrimination. In addition, we also suggest that nucleotide mismatches at the 3'-ends of sense

  20. The Mismatch Negativity: An Indicator of Perception of Regularities in Music

    Xide Yu; Tao Liu; Dingguo Gao

    2015-01-01

    This paper reviews music research using Mismatch Negativity (MMN). MMN is a deviation-specific component of auditory event-related potential (EPR), which detects a deviation between a sound and an internal representation (e.g., memory trace). Recent studies have expanded the notion and the paradigms of MMN to higher-order music processing such as those involving short melodies, harmony chord, and music syntax. In this vein, we firstly reviewed the evolution of MMN from sound to music and then...

  1. Mismatch negativity in children with specific language impairment and auditory processing disorder

    Caroline Nunes Rocha-Muniz; Débora Maria Befi-Lopes; Eliane Schochat

    2015-01-01

    INTRODUCTION: Mismatch negativity, an electrophysiological measure, evaluates the brain's capacity to discriminate sounds, regardless of attentional and behavioral capacity. Thus, this auditory event-related potential is promising in the study of the neurophysiological basis underlying auditory processing.OBJECTIVE: To investigate complex acoustic signals (speech) encoded in the auditory nervous system of children with specific language impairment and compare with children with auditory proce...

  2. Do polymorphisms and haplotypes of mismatch repair genes modulate risk of sporadic colorectal cancer?

    Tulupová, Elena; Kumar, R.; Hánová, Monika; Slyšková, Jana; Pardini, Barbara; Poláková, Veronika; Naccarati, Alessio; Vodičková, Ludmila; Novotný, J.; Halamková, J.; Hemminki, K.; Vodička, Pavel

    2008-01-01

    Roč. 648, 1-2 (2008), s. 40-45. ISSN 0027-5107 R&D Projects: GA ČR GA310/07/1430 Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z50390703 Keywords : DNA mismatch repair * Genetic polymorphism * Haplotype analysis Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.198, year: 2008

  3. Skill Mismatches and Anticipation of the Future Labour Market Need: Case of Croatia

    Bejaković, Predrag; MRNJAVAC, Željko

    2014-01-01

    Skills are the key elements contributing to the prosperity of nations and to better lives for individuals. That is why countries have invested heavily in skills over past decades. For workers, skills mean employability and social mobility. For society, skills represent a major component of its productivity, competitiveness and innovation. An important dimension of labour market disequilibria is the mismatch of supply and demand of different skills at the sectoral, regional and occupational...

  4. Diagonal Loading of Robust General-Rank Beamformer for Direction of Arrival Mismatch

    Khan, Z. U.; A. Naveed; A. Safeer; F. Zaman

    2013-01-01

    This study presents a technique which utilizes the movement of the peak of the main beam towards the presumed signal direction with negative diagonal loading for robust general-rank beamformer. The main beam symmetry along presumed signal direction is improved by this movement. When desired signal is contained in the data snapshots, the conventional beamformers face the problem of performance degradation even if there is a small mismatch between the presumed and the actual signal direction. D...

  5. Regional ventilation/perfusion mismatch pattern in patient with Swyer James (MacLeod's) syndrome

    Sait Sager; Sertac Asa; Reşit Akyel; Ersan Atahan; Bedii Kanmaz

    2014-01-01

    Swyer James (McLeod′s) syndrome (SJMS) is an uncommon disease, which occurs as a result of childhood bronchiolitis obliterans. Patients may not be diagnosed until later in their life. A 46-year-old man underwent ventilation/perfusion scintigraphy for acute onset of dyspnea. The scan showed markedly diminished ventilation and perfusion unilaterally on the right middle and inferior lobes. However, mismatched ventilation-perfusion pattern was shown on the upper right lobe, which was consistent w...

  6. Measurement errors in multifrequency bioelectrical impedance analyzers with and without impedance electrode mismatch.

    Bogónez Franco, Francisco; Nescolarde Selva, Lexa Digna; Bragós Bardia, Ramon; Rosell Ferrer, Francisco Javier; Yandiola, Iñigo

    2009-01-01

    The purpose of this study is to compare measurement errors in two commercially available multi-frequency bioimpedance analyzers, a Xitron 4000B and an ImpediMed SFB7, including electrode impedance mismatch. The comparison was made using resistive electrical models and in ten human volunteers. We used three different electrical models simulating three different body segments: the right-side, leg and thorax. In the electrical models, we tested the effect of the capacitive coupling of the ...

  7. Molecular characteristics of mismatch repair genes in sporadic colorectal tumors in Czech patients

    Vymetálková, Veronika; Slyšková, Jana; Korenková, Vlasta; Bielik, Ludovít; Langerová, Lucie; Procházka, Pavel; Rejhová, Alexandra; Schwarzová, L.; Pardini, B.; Naccarati, Alessio; Vodička, Pavel

    2014-01-01

    Roč. 15, č. 1 (2014), s. 17. ISSN 1471-2350 R&D Projects: GA AV ČR IAA500200917; GA ČR(CZ) GPP304/11/P715 Grant ostatní: GA MŠk(CZ) Prvouk-P27/LF1/1 Institutional support: RVO:68378041 ; RVO:86652036 Keywords : colorectal cancer * mismatch repair genes * expression levels Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.083, year: 2014

  8. Questioning the role of phenology shifts and trophic mismatching in a planktonic food web

    Atkinson, Angus; Harmer, Rachel A.; Widdicombe, Claire E.; McEvoy, Andrea J.; Smyth, Tim J.; Cummings, Denise G.; Somerfield, Paul J.; Maud, Jacqueline L.; McConville, Kristian

    2015-09-01

    In a warming climate, differential shifts in the seasonal timing of predators and prey have been suggested to lead to trophic "mismatches" that decouple primary, secondary and tertiary production. We tested this hypothesis using a 25-year time-series of weekly sampling at the Plymouth L4 site, comparing 57 plankton taxa spanning 4 trophic levels. During warm years, there was a weak tendency for earlier timings of spring taxa and later timings of autumn taxa. While this is in line with many previous findings, numerous exceptions existed and only a few taxa (e.g. Gyrodinium spp., Pseudocalanus elongatus, and Acartia clausi) showed consistent, strong evidence for temperature-related timing shifts, revealed by all 4 of the timing indices that we used. Also, the calculated offsets in timing (i.e. "mismatches") between predator and prey were no greater in extreme warm or cold years than during more average years. Further, the magnitude of these offsets had no effect on the "success" of the predator, in terms of their annual mean abundance or egg production rates. Instead numerous other factors override, including: inter-annual variability in food quantity, high food baseline levels, turnover rates and prolonged seasonal availability, allowing extended periods of production. Furthermore many taxa, notably meroplankton, increased well before the spring bloom. While theoretically a chronic mismatch, this likely reflects trade-offs for example in predation avoidance. Various gelatinous taxa (Phaeocystis, Noctiluca, ctenophores, appendicularians, medusae) may have reduced these predation constraints, with variable, explosive population outbursts likely responding to improved conditions. The match-mismatch hypothesis may apply for highly seasonal, pulsed systems or specialist feeders, but we suggest that the concept is being over-extended to other marine systems where multiple factors compensate.

  9. Mismatch repair protein expression and colorectal cancer in Hispanics from Puerto Rico

    De Jesus-Monge, Wilfredo E.; Gonzalez-Keelan, Carmen; Zhao, Ronghua; Hamilton, Stanley R.; Rodriguez-Bigas, Miguel; Cruz-Correa, Marcia

    2010-01-01

    Colorectal cancer (CRC) is a leading cause of morbidity and mortality and alterations in mismatch repair (MMR) genes, leading to absent protein (negative) expression, are responsible for approximately 20% of CRC cases. Immunohistochemistry is a tool for prescreening of MMR protein expression in CRC but the literature on its use on Hispanics is scarce. However, Hispanics represent the second leading ethnicity in the United States (US) and CRC is a public health burden in this group. Our object...

  10. Theoretical and empirical framework of measuring mismatch on a labour market

    Alka Obadić

    2006-01-01

    The main emphasis of this article is focused on the growing problem of structural unemployment in selected group of transition countries. Namely, if a certain region has a greater degree of unemployment rate than the frictional one, and if another region shows greater number of available vacancies than in conditions of frictionalunemployment, it is referred to as structural disequilibrium or mismatch. Such unemployment is called structural unemployment. The estimates clearly show that the mis...

  11. Learner resistance in metacognition training? An exploration of mismatches between learner and teacher agendas

    J. Huang

    2006-01-01

    This paper examines how and why learner resistance (to the teacher's goals and expectations) occurred in a metacognition-training (MT) project, which aimed to enhance reflection and autonomy in EFL learning. MT was integrated into a regular EFL reading course for second-year BA TEFL undergraduates at a Chinese university. Learner resistance in the MT project was manifested partly through mismatches between the goals and expectations on the part of the teacher and the students. After suggestin...

  12. Variations in mismatch repair genes and colorectal cancer risk and clinical outcome

    Vymetálková, Veronika; Pardini, B.; Rosa, F.; Di Gaetano, C.; Novotný, J.; Levý, M.; Buchler, T.; Slyšková, Jana; Vodičková, Ludmila; Naccarati, Alessio; Vodička, Pavel

    2014-01-01

    Roč. 29, č. 4 (2014), s. 259-265. ISSN 0267-8357 R&D Projects: GA ČR GPP304/11/P715; GA ČR GAP304/10/1286; GA MZd NT12025 Institutional support: RVO:68378041 Keywords : colorectal cancer, , * mismatch repair genes * miRNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.793, year: 2014

  13. Antisynchronization of a novel hyperchaotic system with parameter mismatch and external disturbances

    Fei Yu; Chun Hua Wang; Yan Hu; Jin Wen Yin

    2012-07-01

    A novel hyperchaotic system is proposed. It is particularly interesting that the hyperchaotic system has a nonlinear term in the form of an exponential function and has onle one equilibrium. Basic dynamical properties of the hyperchaotic system are investigated. Moreover, antisynchronization of the new hyperchaotic system with parameter mismatch and external disturbances is also studied in this paper by using adaptive control. Numerical simulation results further demonstrate that the proposed methods are effective and robust.

  14. Orthoesters formation leading to mismatched Helferich glycosylations at O-3 of N-trichloroacetylated glucosamine residues.

    Forman, Adam; Auzanneau, France-Isabelle

    2016-04-29

    Using trisaccharide diol acceptors displaying two glucosamine residues free at O-3, we observed that α-l-fucosylation with α armed donor proceeded smoothly at the most accessible N-trichloroacetyl nonreducing end glucosamine residue. In contrast, glycosylations with peracetylated glycosyl bromide donors activated under Helferich conditions seemed to proceed preferentially or exclusively at the more sterically hindered N-acetylated reducing end unit. Thus, we concluded that disarmed donors were mismatched at O-3 of the N-trichloroacetylated glucosamine residue regardless of α or β configuration of the glycosidic bond formed and d or l configuration of the donor. Interestingly orthoester formation occurred in some cases at this position while they were not observed at the reducing end unit. Conversion of the nonreducing end trichloroacetamido to an acetamido allowed the Helferich catalyzed galactosylation to occur at both positions and revealed the impact of the N-trichloroacetamido on the mismatched glycosylations. Changing the activation conditions from the mild Lewis acid Hg(CN)2 to the stronger acid AgOTf revealed that in fact β-d-galactosylation at the less hindered N-trichloroacetylated residue was kinetically favored over that at the reducing end residue. Isolation of equal amounts of orthoester at this position suggested that it was formed first but that the strong AgOTf Lewis acid was able to promote rearrangement to the β-d-galactosidic bond. These results shed additional light on the apparent mismatch of disarmed glycosyl donors with hydroxyl groups deemed more accessible. Depending on electronic factors imposed by the acceptor and activation conditions, transient unstable orthoester formation may explain in some cases why these donors appear mismatched with the most accessible hydroxyl groups which are otherwise glycosylated by armed donors. PMID:27015141

  15. Mismatch correction modulates mutation frequency and pilus phase and antigenic variation in Neisseria gonorrhoeae.

    Criss, Alison K; Bonney, Kevin M; Chang, Rhoda A; Duffin, Paul M; LeCuyer, Brian E; Seifert, H Steven

    2010-01-01

    The mismatch correction (MMC) system repairs DNA mismatches and single nucleotide insertions or deletions postreplication. To test the functions of MMC in the obligate human pathogen Neisseria gonorrhoeae, homologues of the core MMC genes mutS and mutL were inactivated in strain FA1090. No mutH homologue was found in the FA1090 genome, suggesting that gonococcal MMC is not methyl directed. MMC mutants were compared to a mutant in uvrD, the helicase that functions with MMC in Escherichia coli. Inactivation of MMC or uvrD increased spontaneous resistance to rifampin and nalidixic acid, and MMC/uvrD double mutants exhibited higher mutation frequencies than any single mutant. Loss of MMC marginally enhanced the transformation efficiency of DNA carrying a single nucleotide mismatch but not that of DNA with a 1-kb insertion. Unlike the exquisite UV sensitivity of the uvrD mutant, inactivating MMC did not affect survival after UV irradiation. MMC and uvrD mutants exhibited increased PilC-dependent pilus phase variation. mutS-deficient gonococci underwent an increased frequency of pilin antigenic variation, whereas uvrD had no effect. Recombination tracts in the mutS pilin variants were longer than in parental gonococci but utilized the same donor pilS loci. These results show that gonococcal MMC repairs mismatches and small insertion/deletions in DNA and also affects the recombination events underlying pilin antigenic variation. The differential effects of MMC and uvrD in gonococci unexpectedly reveal that MMC can function independently of uvrD in this human-specific pathogen. PMID:19854909

  16. Predicting the Flory-Huggins χ Parameter for Polymers with Stiffness Mismatch from Molecular Dynamics Simulations

    Daniel J. Kozuch; Wenlin Zhang; Milner, Scott T.

    2016-01-01

    The Flory–Huggins χ parameter describes the excess free energy of mixing and governs phase behavior for polymer blends and block copolymers. For chemically-distinct nonpolar polymers, the value of χ is dominated by the mismatch in cohesive energy densities of the monomers. For blends of chemically-similar polymers, the entropic portion of χ, arising from non-ideal local packing, becomes more significant. Using polymer field theory, Fredrickson et al. predicted that a difference in backbone st...

  17. Mismatch response to polysyllabic nonwords: a neurophysiological signature of language learning capacity.

    Johanna G Barry

    Full Text Available BACKGROUND: The ability to repeat polysyllabic nonwords such as "blonterstaping" has frequently been shown to correlate with language learning ability but it is not clear why such a correlation should exist. Three alternative explanations have been offered, stated in terms of differences in: (a perceptual ability; (b efficiency of phonological loop functioning; (c pre-existing vocabulary knowledge and/or articulatory skills. In the present study, we used event-related potentials to assess the contributions from these three factors to explaining individual variation in nonword repetition ability. METHODOLOGY/PRINCIPAL FINDINGS: 59 adults who were subdivided according to whether they were good or poor nonword-repeaters participated. Electrophysiologically measured mismatch responses were recorded to changes in consonants as participants passively listened to a repeating four syllable CV-string. The consonant change could occur in one of four positions along the CV-string and we predicted that: (a if nonword repetition depended purely on auditory discrimination ability, then reduced mismatch responses to all four consonant changes would be observed in the poor nonword-repeaters, (b if it depended on encoding or decay of information in a capacity-limited phonological store, then a position specific decrease in mismatch response would be observed, (c if neither cognitive capacity was involved, then the two groups of participants would provide equivalent mismatch responses. Consistent with our second hypothesis, a position specific difference located on the third syllable was observed in the late discriminative negativity (LDN window (230-630 ms post-syllable onset. CONCLUSIONS/SIGNIFICANCE: Our data thus confirm that people who are poorer at nonword repetition are less efficient in early processing of polysyllabic speech materials, but this impairment is not attributable to deficits in low level auditory discrimination. We conclude by discussing the

  18. Mismatch Negativity/P3a Complex in Young People with Psychiatric Disorders: A Cluster Analysis

    Kaur, Manreena; Lagopoulos, Jim; Ward, Philip B; Watson, Tamara L; Naismith, Sharon L; Hickie, Ian B; Hermens, Daniel F.

    2012-01-01

    Background We have recently shown that the event-related potential biomarkers, mismatch negativity (MMN) and P3a, are similarly impaired in young patients with schizophrenia- and affective-spectrum psychoses as well as those with bipolar disorder. A data driven approach may help to further elucidate novel patterns of MMN/P3a amplitudes that characterise distinct subgroups in patients with emerging psychiatric disorders. Methods Eighty seven outpatients (16 to 30 years) were assessed: 19 diagn...

  19. Cortical Encoding of Pitch Contour Changes in Cochlear Implant Users: A Mismatch Negativity Study

    Zhang, Fawen; Benson, Chelsea; Fu, Qian-Jie

    2013-01-01

    A better understanding of melodic pitch perception in cochlear implants (CIs) may guide signal processing and/or rehabilitation techniques to improve CI patients' music perception and appreciation. In this study, the mismatch negativity (MMN) in response to infrequent changes in five-tone pitch contours was obtained in CI users and normal hearing (NH) listeners. Melodic contour identification (MCI) was also measured. Results showed that MCI performance was poorer in CI subjects than in NH sub...

  20. Agricultural intensification exacerbates spillover effects on soil biogeochemistry in adjacent forest remnants.

    Raphael K Didham

    Full Text Available Land-use intensification is a central element in proposed strategies to address global food security. One rationale for accepting the negative consequences of land-use intensification for farmland biodiversity is that it could 'spare' further expansion of agriculture into remaining natural habitats. However, in many regions of the world the only natural habitats that can be spared are fragments within landscapes dominated by agriculture. Therefore, land-sparing arguments hinge on land-use intensification having low spillover effects into adjacent protected areas, otherwise net conservation gains will diminish with increasing intensification. We test, for the first time, whether the degree of spillover from farmland into adjacent natural habitats scales in magnitude with increasing land-use intensity. We identified a continuous land-use intensity gradient across pastoral farming systems in New Zealand (based on 13 components of farmer input and soil biogeochemistry variables, and measured cumulative off-site spillover effects of fertilisers and livestock on soil biogeochemistry in 21 adjacent forest remnants. Ten of 11 measured soil properties differed significantly between remnants and intact-forest reference sites, for both fenced and unfenced remnants, at both edge and interior. For seven variables, the magnitude of effects scaled significantly with magnitude of surrounding land-use intensity, through complex interactions with fencing and edge effects. In particular, total C, total N, δ15N, total P and heavy-metal contaminants of phosphate fertilizers (Cd and U increased significantly within remnants in response to increasing land-use intensity, and these effects were exacerbated in unfenced relative to fenced remnants. This suggests movement of livestock into surrounding natural habitats is a significant component of agricultural spillover, but pervasive changes in soil biogeochemistry still occur through nutrient spillover channels alone

  1. Carbon sources supporting benthic mineralization in mangrove and adjacent seagrass sediments (Gazi Bay, Kenya

    F. Dehairs

    2004-08-01

    Full Text Available The origin of carbon substrates used by in situ sedimentary bacterial communities was investigated in an intertidal mangrove ecosystem and in adjacent seagrass beds in Gazi bay (Kenya by δ13C analysis of bacteria-specific PLFA (phospholipid fatty acids and bulk organic carbon. Export of mangrove-derived organic matter to the adjacent seagrass-covered bay was evident from sedimentary total organic carbon (TOC and δ13CTOC data. PLFA δ13C data indicate that the substrate used by bacterial communities varied strongly and that exported mangrove carbon was a significant source for bacteria in the adjacent seagrass beds. Within the intertidal mangrove forest, bacterial PLFA at the surface layer (0-1 cm typically showed more enriched δ13C values than deeper (up to 10 cm sediment layers, suggesting a contribution from microphytobenthos and/or inwelled seagrass material. Under the assumption that seagrasses and mangroves are the dominant potential end-members, the estimated contribution of mangrove-derived carbon to benthic mineralization in the seagrass beds (16-74% corresponds fairly well to the estimated contribution of mangrove C to the sedimentary organic matter pool (21-71% across different seagrass sites. Based on these results and a compilation of literature data, we suggest that allochtonous carbon trapped in seagrass beds may often represent a significant fraction of the substrate for benthic mineralization - both in cases where seagrass C dominates the sediment TOC pool and in cases where external inputs are significant. Hence, it is likely that community respiration data systematically overestimate the role of mineralization in the overall seagrass C budget.

  2. Carbon sources supporting benthic mineralization in mangrove and adjacent seagrass sediments (Gazi Bay, Kenya

    S. Bouillon

    2004-01-01

    Full Text Available The origin of carbon substrates used by in situ sedimentary bacterial communities was investigated in an intertidal mangrove ecosystem and in adjacent seagrass beds in Gazi bay (Kenya by δ13C analysis of bacteria-specific PLFA (phospholipid fatty acids and bulk organic carbon. Export of mangrove-derived organic matter to the adjacent seagrass-covered bay was evident from sedimentary total organic carbon (TOC and δ13CTOC data. PLFA δ13C data indicate that the substrate used by bacterial communities varied strongly and that exported mangrove carbon was a significant source for bacteria in the adjacent seagrass beds. Within the intertidal mangrove forest, bacterial PLFA at the surface layer (0-1cm typically showed more enriched δ13C values than deeper (up to 10cm sediment layers, suggesting a contribution from microphytobenthos and/or inwelled seagrass material. Under the simplifying assumption that seagrasses and mangroves are the dominant potential end-members, the estimated contribution of mangrove-derived carbon to benthic mineralization in the seagrass beds (16-74% corresponds fairly well to the estimated contribution of mangrove C to the sedimentary organic matter pool (21-71% across different seagrass sites. Based on the results of this study and a compilation of literature data, we suggest that trapping of allochtonous C is a common feature in seagrass beds and often represents a significant source of C for sediment bacteria - both in cases where seagrass C dominates the sediment TOC pool and in cases where external inputs are significant. Hence, it is likely that data on community respiration rates systematically overestimate the role of in situ mineralization as a fate of seagrass production.

  3. Influence of framework design, contraction mismatch, and thermal history on porcelain checking in fixed partial dentures.

    Anusavice, K J; Gray, A E

    1989-01-01

    The objective of this study was to characterize the relative influence of contraction mismatch, framework design, furnace type, cooling rate, and multiple firings on immediate or delayed checking in fixed partial dentures. Frameworks for 60 anterior bridges (three-unit fixed partial dentures) were cast from a low-expansion Au-Pd alloy (O) and a high-expansion Pd-Ag alloy (J). A high-expansion porcelain (B) was applied to each of three framework designs. Firing was performed at heating rates of 56 degrees C/min and 180 degrees C/min. Specimens were cooled at two rates after each of five glazing cycles. For O-B specimens which exhibited a negative thermal contraction mismatch between 600 degrees C and 25 degrees C, 60% of the bridge specimens failed when they were subjected to slow cooling preceded by either fast or slow heating. When J-B specimens (which exhibited a smaller negative contraction mismatch) were heated and cooled rapidly, no failures occurred through all of the firing cycles. However, cracks were observed in 13.3% of the J-B bridges which were slowly heated and rapidly cooled. Delayed cracks (after the fifth glaze cycle) developed over periods of up to two years only in bridges which were slowly cooled in the furnace chamber. The results of this study suggest that checking in conventional feldspathic porcelains can be promoted by slow cooling rates and an excessive number of firing cycles. PMID:2691298

  4. Analysis of mismatch and shading effects in a photovoltaic array using different technologies

    In this paper, we analyze the performance of a photovoltaic array implemented in the Universidad Politécnica de Valencia which consists of modules of different technologies and power, connected in series, in order to quantify the energy losses due to mismatch and the effect of the shadows. To do this, the performance of the modules was measured in operation under ambient conditions with field measurement equipment (AMPROBE Solar Analyzer, Solar - 4000), which allows the extrapolation of measures to standard conditions STC. For the data validation, measures under controlled conditions were taken to some modules in the flash test laboratory of the Institute of Energy Technology ITE of Valencia in Spain. Subsequently the array curves measured were validated with a photovoltaic array model developed in MATLAB-Simulink for the same conditions and technologies. The results of this particular array are lost up to 20% of the energy supplied due to the modules mismatch. The study shows the curves and the energy loss due to shadows modules. This result opens scenarios for conceivable modifications to the PV field configurations today, chosen during the design stage and unchangeable during the operating stage; and gives greater importance to the energy loss by mismatch in the PV array

  5. Auditory cortical and hippocampal-system mismatch responses to duration deviants in urethane-anesthetized rats.

    Timo Ruusuvirta

    Full Text Available Any change in the invariant aspects of the auditory environment is of potential importance. The human brain preattentively or automatically detects such changes. The mismatch negativity (MMN of event-related potentials (ERPs reflects this initial stage of auditory change detection. The origin of MMN is held to be cortical. The hippocampus is associated with a later generated P3a of ERPs reflecting involuntarily attention switches towards auditory changes that are high in magnitude. The evidence for this cortico-hippocampal dichotomy is scarce, however. To shed further light on this issue, auditory cortical and hippocampal-system (CA1, dentate gyrus, subiculum local-field potentials were recorded in urethane-anesthetized rats. A rare tone in duration (deviant was interspersed with a repeated tone (standard. Two standard-to-standard (SSI and standard-to-deviant (SDI intervals (200 ms vs. 500 ms were applied in different combinations to vary the observability of responses resembling MMN (mismatch responses. Mismatch responses were observed at 51.5-89 ms with the 500-ms SSI coupled with the 200-ms SDI but not with the three remaining combinations. Most importantly, the responses appeared in both the auditory-cortical and hippocampal locations. The findings suggest that the hippocampus may play a role in (cortical manifestation of MMN.

  6. An Algorithm for Quantitatively Calculating I/Q Gain and Phase Mismatch

    Jin Liu

    2012-08-01

    Full Text Available The in-phase and quadrature modulator (IQ modulator is a key component in modern wireless transmitter. It provides a convenient method for modulating data bits or symbols onto an RF carrier. It has become the architecture of choice for implementing transmitter signal chains for end applications such as cellular, WiMAX, and wireless point-to-point. However, there are several non-ideal aspects of analog IQ modulator, include IQ gain imbalance, imperfect quadrature, and LO leakage. These imperfections will result in image spectral and degrade MER of the modulator, which in turn degrades bit error rate (BER. Gain matching and phase compensation both affect the total amount of image rejection. Factory calibration can detect the IQ gain and phase mismatch in different frequency and store the correction coefficients in nonvolatile memory. The key issue is how to get the exact mismatch. In this paper, a mathematical model of IQ modulator was established and an analytic solution is obtained about how to get the exact mismatch parameters.

  7. Mismatch or allostatic load? Timing of life adversity differentially shapes gray matter volume and anxious temperament.

    Kuhn, Manuel; Scharfenort, Robert; Schümann, Dirk; Schiele, Miriam A; Münsterkötter, Anna L; Deckert, Jürgen; Domschke, Katharina; Haaker, Jan; Kalisch, Raffael; Pauli, Paul; Reif, Andreas; Romanos, Marcel; Zwanzger, Peter; Lonsdorf, Tina B

    2016-04-01

    Traditionally, adversity was defined as the accumulation of environmental events (allostatic load). Recently however, a mismatch between the early and the later (adult) environment (mismatch) has been hypothesized to be critical for disease development, a hypothesis that has not yet been tested explicitly in humans. We explored the impact of timing of life adversity (childhood and past year) on anxiety and depression levels (N = 833) and brain morphology (N = 129). Both remote (childhood) and proximal (recent) adversities were differentially mirrored in morphometric changes in areas critically involved in emotional processing (i.e. amygdala/hippocampus, dorsal anterior cingulate cortex, respectively). The effect of adversity on affect acted in an additive way with no evidence for interactions (mismatch). Structural equation modeling demonstrated a direct effect of adversity on morphometric estimates and anxiety/depression without evidence of brain morphology functioning as a mediator. Our results highlight that adversity manifests as pronounced changes in brain morphometric and affective temperament even though these seem to represent distinct mechanistic pathways. A major goal of future studies should be to define critical time periods for the impact of adversity and strategies for intervening to prevent or reverse the effects of adverse childhood life experiences. PMID:26568620

  8. Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex.

    Banerjee, Sreeparna; Flores-Rozas, Hernan

    2005-01-01

    Cadmium (Cd2+) is a known carcinogen that inactivates the DNA mismatch repair (MMR) pathway. In this study, we have tested the effect of Cd2+ exposure on the enzymatic activity of the mismatch binding complex MSH2-MSH6. Our results indicate that Cd2+ is highly inhibitory to the ATP binding and hydrolysis activities of MSH2-MSH6, and less inhibitory to its DNA mismatch binding activity. The inhibition of the ATPase activity appears to be dose and exposure time dependent. However, the inhibition of the ATPase activity by Cd2+ is prevented by cysteine and histidine, suggesting that these residues are essential for the ATPase activity and are targeted by Cd2+. A comparison of the mechanism of inhibition with N-ethyl maleimide, a sulfhydryl group inhibitor, indicates that this inhibition does not occur through direct inactivation of sulfhydryl groups. Zinc (Zn2+) does not overcome the direct inhibitory effect of Cd2+ on the MSH2-MSH6 ATPase activity in vitro. However, the increase in the mutator phenotype of yeast cells exposed to Cd2+ was prevented by excess Zn2+, probably by blocking the entry of Cd2+ into the cell. We conclude that the inhibition of MMR by Cd2+ is through the inactivation of the ATPase activity of the MSH2-MSH6 heterodimer, resulting in a dominant negative effect and causing a mutator phenotype. PMID:15746000

  9. Mismatch repair inhibits homeologous recombination via coordinated directional unwinding of trapped DNA structures.

    Tham, Khek-Chian; Hermans, Nicolaas; Winterwerp, Herrie H K; Cox, Michael M; Wyman, Claire; Kanaar, Roland; Lebbink, Joyce H G

    2013-08-01

    Homeologous recombination between divergent DNA sequences is inhibited by DNA mismatch repair. In Escherichia coli, MutS and MutL respond to DNA mismatches within recombination intermediates and prevent strand exchange via an unknown mechanism. Here, using purified proteins and DNA substrates, we find that in addition to mismatches within the heteroduplex region, secondary structures within the displaced single-stranded DNA formed during branch migration within the recombination intermediate are involved in the inhibition. We present a model that explains how higher-order complex formation of MutS, MutL, and DNA blocks branch migration by preventing rotation of the DNA strands within the recombination intermediate. Furthermore, we find that the helicase UvrD is recruited to directionally resolve these trapped intermediates toward DNA substrates. Thus, our results explain on a mechanistic level how the coordinated action between MutS, MutL, and UvrD prevents homeologous recombination and maintains genome stability. PMID:23932715

  10. A beamformer analysis of MEG data reveals frontal generators of the musically elicited mismatch negativity.

    Claudia Lappe

    Full Text Available To localize the neural generators of the musically elicited mismatch negativity with high temporal resolution we conducted a beamformer analysis (Synthetic Aperture Magnetometry, SAM on magnetoencephalography (MEG data from a previous musical mismatch study. The stimuli consisted of a six-tone melodic sequence comprising broken chords in C- and G-major. The musical sequence was presented within an oddball paradigm in which the last tone was lowered occasionally (20% by a minor third. The beamforming analysis revealed significant right hemispheric neural activation in the superior temporal (STC, inferior frontal (IFC, superior frontal (SFC and orbitofrontal (OFC cortices within a time window of 100-200 ms after the occurrence of a deviant tone. IFC and SFC activation was also observed in the left hemisphere. The pronounced early right inferior frontal activation of the auditory mismatch negativity has not been shown in MEG studies so far. The activation in STC and IFC is consistent with earlier electroencephalography (EEG, optical imaging and functional magnetic resonance imaging (fMRI studies that reveal the auditory and inferior frontal cortices as main generators of the auditory MMN. The observed right hemispheric IFC is also in line with some previous music studies showing similar activation patterns after harmonic syntactic violations. The results demonstrate that a deviant tone within a musical sequence recruits immediately a distributed neural network in frontal and prefrontal areas suggesting that top-down processes are involved when expectation violation occurs within well-known stimuli.

  11. High-Frame-Rate Synthetic Aperture Ultrasound Imaging Using Mismatched Coded Excitation Waveform Engineering: A Feasibility Study.

    Lashkari, Bahman; Zhang, Kaicheng; Mandelis, Andreas

    2016-06-01

    Mismatched coded excitation (CE) can be employed to increase the frame rate of synthetic aperture ultrasound imaging. The high autocorrelation and low cross correlation (CC) of transmitted signals enables the identification and separation of signal sources at the receiver. Thus, the method provides B-mode imaging with simultaneous transmission from several elements and capability of spatial decoding of the transmitted signals, which makes the imaging process equivalent to consecutive transmissions. Each transmission generates its own image and the combination of all the images results in an image with a high lateral resolution. In this paper, we introduce two different methods for generating multiple mismatched CEs with an identical frequency bandwidth and code length. Therefore, the proposed families of mismatched CEs are able to generate similar resolutions and signal-to-noise ratios. The application of these methods is demonstrated experimentally. Furthermore, several techniques are suggested that can be used to reduce the CC between the mismatched codes. PMID:27101603

  12. Role of Cell Cycle Regulation and MLH1, A Key DNA Mismatch Repair Protein, In Adaptive Survival Responses. Final Report

    David A. Boothman

    1999-08-11

    Due to several interesting findings on both adaptive survival responses (ASRs) and DNA mismatch repair (MMR), this grant was separated into two discrete Specific Aim sets (each with their own discrete hypotheses). The described experiments were simultaneously performed.

  13. Particulate Matter Levels in Ambient Air Adjacent to Industrial Area

    Mohamed, R. M. S. R.; Nizam, N. M. S.; Al-Gheethi, A. A.; Lajis, A.; Kassim, A. H. M.

    2016-07-01

    Air quality in the residential areas adjacent to the industrial regions is of great concern due to the association with human health risks. In this work, the concentrations of particulate matter (PM10) in the ambient air of UTHM campus was investigated tostudy the air qualityand their compliance to the Malaysian Ambient Air Quality Guidelines (AAQG). The PM10 samples were taken over 24 hours from the most significant area at UTHM including Stadium, KolejKediamanTunDr. Ismail (KKTDI) and MakmalBahan. The meteorological parameters; temperature, relative humidity, wind speed and wind direction as well as particulate matterwere estimated by using E-Sampler Particulate Matter (PM10) Collector. The highest concentrations of PM10 (55.56 µg/m3) was recorded at MakmalBahan during the working and weekend days. However, these concentrations are less than 150 pg/m3. It can be concluded that although UTHM is surrounded by the industrial area, the air quality in the campus still within the standards limits.

  14. Seismic responses of two adjacent buildings. II. Interaction

    Celebi, Mehmet

    1993-01-01

    Presented in this part of the two-part paper is a study of the relations between earthquake motions recorded from two, adjacent, seven-story buildings, from a downhole below the foundation of one of the buildings and from three free-field sites, all within one city block. This unique data set was obtained during the Whittier-Narrows, Calif. earthquake of Oct. 1, 1987, Part I includes background information on the two buildings, the site, and the data set. Building response characteristics of a code-type instrumented building (A) and an extensively instrumented building (B) are also studied. In this part, spectral analysis techniques are used to study the relationships between the motions of the roofs and basements, the downhole and the free-field sites. It is asserted that there is building-soil-building interaction between the two buildings at a frequency of 2.35 Hz. Furthermore, the free-field motions are shown to be influenced by the presence of the buildings.

  15. Can Logging in Equatorial Africa Affect Adjacent Parks?

    Jeremy W. Lichstein

    2005-06-01

    Full Text Available Tropical deforestation can cause fundamental regional-scale shifts in vegetation structure and diversity. This is particularly true in Africa. Although national parks are being established to protect areas from deforestation and to conserve biodiversity, these parks are not immune to disturbances outside their boundaries. We used regional-scale atmospheric simulation experiments to investigate how deforestation in timber concessions might affect precipitation inside adjacent, undisturbed national parks in the equatorial African countries of Gabon and the Republic of Congo. The experiments revealed a complex response. Some parks showed rainfall reduced as much as 15%, while others showed slight increases. Rainfall inside parks was particularly sensitive to upwind deforestation along the path of airborne moisture traveling inland from the ocean. A variety of shortcomings in the current modeling procedures limit the ability to extrapolate from experiments such as ours to provide spatially explicit, long-term forecasts of climate. We describe what advances in modeling are needed to produce regional-scale predictions that are robust enough to be useful to managers and policy makers.

  16. Adjacent-cell preconditioners for accelerating multidimensional neutron transport methods

    The Adjacent-cell Preconditioner (AP) is derived for accelerating generic fixed-weight, Weighted Diamond Difference (WDD) neutron transport methods in multidimensional Cartesian geometry. The AP is determined by requiring: (a) the eigenvalue of the combined mesh sweep-AP iterations to vanish in the vicinity of the origin in Fourier space; and (b) the diagonal and off-diagonal elements of the preconditioner to satisfy a diffusion-like condition. The spectra of the resulting iterations for a wide range of problem parameters exhibit a spectral radius smaller than .25, that vanishes implying immediate convergence for very large computational cells. More importantly, unlike other unconditionally stable acceleration schemes, the AP is cell-centered and its spectral radius remains small when the cell aspect ratio approaches 0 or ∞. Testing of the AP and comparison of its rate of convergence to the standard Source Iterations (SI) for Burre's Suite of Test Problems (BSTeP) demonstrates its high efficiency in reducing the number of iterations required to achieve convergence, especially for optically thick cells where acceleration is most needed

  17. Impact of shield tunneling on adjacent spread foundation on sandy cobble strata

    Yong Fang; Jun Wang; Chuan He; Xiongyu Hu

    2014-01-01

    The section of shield tunnel of the Chengdu Metro line passes primarily through sandy cobble strata. There are many buildings with spread foundations along the lines. Shield tunnel construction will disturb the ground, causing displacement or stress to adjacent spread foundations. Based on the similarity theory, a laboratory model test of shield tunnel driving was carried out to study the influence of shield tunnel excavation on the displace-ment of adjacent spread foundation. The results show that foundation closer to the tunnel has greater displacement or settlement than that further away. The horizontal dis-placement is small and is influenced greatly by the cutting face. The displacement along the machine driving direction is bigger and is significantly affected by the thrust force. Settlement occurs primarily when shield machine passes close to the foundation and is the greatest at that time. Uneven settlement at the bottom of the spread foundation reaches a maximum after the excavation ends. In a numerical simulation, a particle flow model was con-structed to study the impact of shield tunnel excavation on the stresses in the ground. The model showed stress con-centration at the bottom of the spread foundation. With the increasing ground loss ratio, a loose area appears in the tunnel dome where the contact force dropped. Above the loose area, the contact force increases, forming an arch-shaped soil area which prevents the loose area from expanding to the ground surface. The excavation also changed the pressure distribution around spread foundation.

  18. The tiger beetles (Coleoptera, Carabidae, Cicindelinae) of Israel and adjacent lands

    Matalin, Andrey V.; Chikatunov, Vladimir I.

    2016-01-01

    Abstract Based on field studies, museums collections and literature sources, the current knowledge of the tiger beetle fauna of Israel and adjacent lands is presented. In Israel eight species occur, one of them with two subspecies, while in the Sinai Peninsula nine species of tiger beetles are now known. In the combined regions seven genera from two tribes were found. The Rift Valley with six cicindelids species is the most specious region of Israel. Cylindera contorta valdenbergi and Cicindela javeti azari have localized distributions and should be considered regional endemics. A similarity analysis of the tiger beetles faunas of different regions of Israel and the Sinai Peninsula reveal two clusters of species. The first includes the Great Rift Valley and most parts of the Sinai Peninsula, and the second incorporates most regions of Israel together with Central Sinai Foothills. Five distinct adult phenological groups of tiger beetles can be distinguished in these two clusters: active all-year (three species), spring-fall (five species), summer (two species), spring-summer (one species) and spring (one species). The likely origins of the tiger beetle fauna of this area are presented. An annotated list and illustrated identification key of the Cicindelinae of Israel and adjacent lands are provided. PMID:27110198

  19. Diversity of fungi from the mound nests of Formica ulkei and adjacent non-nest soils.

    Duff, Lyndon B; Urichuk, Theresa M; Hodgins, Lisa N; Young, Jocelyn R; Untereiner, Wendy A

    2016-07-01

    Culture-based methods were employed to recover 3929 isolates of fungi from soils collected in May and July 2014 from mound nests of Formica ulkei and adjacent non-nest sites. The abundance, diversity, and richness of species from nest mounds exceeded those of non-mound soils, particularly in July. Communities of fungi from mounds were more similar to those from mounds than non-mounds; this was also the case for non-mound soils with the exception of one non-mound site in July. Species of Aspergillus, Paecilomyces, and Penicillium were dominant in nest soils and represented up to 81.8% of the taxa recovered. Members of the genus Aspergillus accounted for the majority of Trichocomaceae from nests and were represented almost exclusively by Aspergillus navahoensis and Aspergillus pseudodeflectus. Dominant fungi from non-mound sites included Cladosporium cladosporioides, Geomyces pannorum, and species of Acremonium, Fusarium, Penicillium, and Phoma. Although mound nests were warmer than adjacent soils, the dominance of xerotolerant Aspergillus in soils from mounds and the isolation of the majority of Trichocomaceae at 25 and 35 °C suggests that both temperature and water availability may be determinants of fungal community structure in nests of F. ulkei. PMID:27192606

  20. A Regional Ocean Reanalysis System for Coastal Waters of China and Adjacent Seas

    HAN Guijun; LI Wei; ZHANG Xuefeng; LI Dong; HE Zhongjie; WANG Xidong; WU Xinrong; YU Ting; MA Jirui

    2011-01-01

    A regional ocean reanalysis system for the coastal waters of China and adjacent seas has been developed by the National Marine Data and Information Service (NMDIS). It produces a dataset package called CORA (China ocean reanalysis). The regional ocean model used is based on the Princeton Ocean Model with a generalized coordinate system (POMgcs). The model is parallelized by NMDIS with the addition of the wave breaking and tidal mixing processes into model parameterizations. Data assimilation is a sequential three-dimensional variational (3D-Var) scheme implemented within a multigrid framework. Observations include satellite remote sensing sea surface temperature (SST), altimetry sea level anomaly (SLA), and temperature/salinity profiles. The reanalysis fields of sea surface height, temperature, salinity, and currents begin with January 1986 and are currently updated every year. Error statistics and error distributions of temperature, salinity and currents are presented as a primary evaluation of the reanalysis fields using sea level data from tidal gauges, temperature profiles, as well as the trajectories of Argo floats. Some case studies offer the opportunity to verify the evolution of certain local circulations. These evaluations show that the reanalysis data produced provide a good representation of the ocean processes and phenomena in the coastal waters of China and adjacent seas.