Methods and Software Architecture for Activity Recognition from Position Data

DEFF Research Database (Denmark)

Godsk, Torben

This thesis describes my studies on the subject of recognizing cow activities from satellite based position data. The studies comprise methods and software architecture for activity recognition from position data, applied to cow activity recognition. The development of methods and software....... The results of these calculations are applied to a given standard machine learning algorithm, and the activity, performed by the cow as the measurements were recorded, is recognized. The software architecture integrates these methods and ensures flexible activity recognition. For instance, it is flexible...... in relation to the use of different sensors modalities and/or within different domains. In addition, the methods and their integration with the software architecture ensures both robust and accurate activity recognition. Utilized, it enables me to classify the five activities robustly and with high success...

Insulator function and topological domain border strength scale with architectural protein occupancy

Science.gov (United States)

2014-01-01

Background Chromosome conformation capture studies suggest that eukaryotic genomes are organized into structures called topologically associating domains. The borders of these domains are highly enriched for architectural proteins with characterized roles in insulator function. However, a majority of architectural protein binding sites localize within topological domains, suggesting sites associated with domain borders represent a functionally different subclass of these regulatory elements. How topologically associating domains are established and what differentiates border-associated from non-border architectural protein binding sites remain unanswered questions. Results By mapping the genome-wide target sites for several Drosophila architectural proteins, including previously uncharacterized profiles for TFIIIC and SMC-containing condensin complexes, we uncover an extensive pattern of colocalization in which architectural proteins establish dense clusters at the borders of topological domains. Reporter-based enhancer-blocking insulator activity as well as endogenous domain border strength scale with the occupancy level of architectural protein binding sites, suggesting co-binding by architectural proteins underlies the functional potential of these loci. Analyses in mouse and human stem cells suggest that clustering of architectural proteins is a general feature of genome organization, and conserved architectural protein binding sites may underlie the tissue-invariant nature of topologically associating domains observed in mammals. Conclusions We identify a spectrum of architectural protein occupancy that scales with the topological structure of chromosomes and the regulatory potential of these elements. Whereas high occupancy architectural protein binding sites associate with robust partitioning of topologically associating domains and robust insulator function, low occupancy sites appear reserved for gene-specific regulation within topological domains. PMID

The amyloid architecture provides a scaffold for enzyme-like catalysts.

Science.gov (United States)

Al-Garawi, Z S; McIntosh, B A; Neill-Hall, D; Hatimy, A A; Sweet, S M; Bagley, M C; Serpell, L C

2017-08-03

Natural biological enzymes possess catalytic sites that are generally surrounded by a large three-dimensional scaffold. However, the proportion of the protein molecule that participates in the catalytic reaction is relatively small. The generation of artificial or miniature enzymes has long been a focus of research because enzyme mimetics can be produced with high activity at low cost. These enzymes aim to mimic the active sites without the additional architecture contributed by the protein chain. Previous work has shown that amyloidogenic peptides are able to self-assemble to create an active site that is capable of binding zinc and catalysing an esterase reaction. Here, we describe the structural characterisation of a set of designed peptides that form an amyloid-like architecture and reveal that their capability to mimic carbonic anhydrase and serve as enzyme-like catalysts is related to their ability to self-assemble. These amyloid fibril structures can bind the metal ion Zn 2+ via a three-dimensional arrangement of His residues created by the amyloid architecture. Our results suggest that the catalytic efficiency of amyloid-like assembly is not only zinc-dependent but also depends on an active centre created by the peptides which is, in turn, dependent on the ordered architecture. These fibrils have good esterase activity, and they may serve as good models for the evolution of modern-day enzymes. Furthermore, they may be useful in designing self-assembling fibrils for applications as metal ion catalysts. This study also demonstrates that the ligands surrounding the catalytic site affect the affinity of the zinc-binding site to bind the substrate contributing to the enzymatic activity of the assembled peptides.

Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids.

Science.gov (United States)

Wu, Xiuyun; Tian, Zhennan; Jiang, Xukai; Zhang, Qun; Wang, Lushan

2018-01-01

XynB from Aspergillus niger ATCC1015 (AnXynB) is a mesophilic glycoside hydrolase (GH) family 11 xylanase which holds great potentials in a wide variety of industrial applications. In the present study, the catalytic activity and stability of AnXynB were improved by a combination of computational and experimental approaches. Virtual mutation and molecular dynamics simulations indicated that the introduction of Glu and Asn altered the interaction network at the - 3 subsite. Interestingly, the double mutant S41N/T43E displayed 72% increase in catalytic activity when compared to the wild type (WT). In addition, it also showed a better thermostability than the WT enzyme. Kinetic determination of the T43E and S41N/T43E mutants suggested that the higher xylanase activity is probably due to the increasing binding affinity of enzyme and substrate. Consequently, the enzyme activity and thermostability of AnXynB was both increased by selective site-directed mutagenesis at the - 3 subsite of its active site architecture which provides a good example for a successfully engineered enzyme for potential industrial application. Moreover, the molecular evolution approach adopted in this study led to the design of a library of sequences that captures a meaningful functional diversity in a limited number of protein variants.

Computational Strategies for the Architectural Design of Bending Active Structures

DEFF Research Database (Denmark)

Tamke, Martin; Nicholas, Paul

2013-01-01

Active bending introduces a new level of integration into the design of architectural structures, and opens up new complexities for the architectural design process. In particular, the introduction of material variation reconfigures the design space. Through the precise specification...

Architecture and Development of DCMP Web Site

Science.gov (United States)

Bariakhtar, Irina

2003-03-01

The multi-tier implementation of DCMP Web site is discussed. It is based upon newly developed PHP technology. The technology allows for creating dynamic content and scalable solutions for Web site capabilities. There are several aspects as to what type of information is to be on the site. First, it should serve the immediate needs of the researchers in the field, namely, conferences, journals, news, funds, etc. This is currently available on the site, but can be extended and improved if needed. Second, the site will reflect the connection between Condensed matter physics and the technological breakthroughs that drive the economy. Third, the site will carry an educational mission helping educate the general public, and on the other hand, help young people to start their careers in the field. The content of the DCMP Web site is under active development. It depends upon wide involvement of DCMP members.

Architectural, Mechanical, Electrical, Plumbing, and Site Documents Associated with Sustainability Base, N232

Science.gov (United States)

Grymes, Rosalind A.; Martin, Rodney Alexander; Dianati, Soheila

2016-01-01

These files contain more precise and accurate representations of the architectural, mechanical, electrical, plumbing, and site information pertaining to Sustainability Base, aka Collaborative Support Facility, aka N232. These supersede the 'bid' drawings released in STI 8112 previously. They are useful for NASA researchers and collaborators in modeling the performance characteristics of the facility. Otherwise, they do not contain new data.

Active site electrostatics protect genome integrity by blocking abortive hydrolysis during DNA recombination

Science.gov (United States)

Ma, Chien-Hui; Rowley, Paul A; Macieszak, Anna; Guga, Piotr; Jayaram, Makkuni

2009-01-01

Water, acting as a rogue nucleophile, can disrupt transesterification steps of important phosphoryl transfer reactions in DNA and RNA. We have unveiled this risk, and identified safeguards instituted against it, during strand cleavage and joining by the tyrosine site-specific recombinase Flp. Strand joining is threatened by a latent Flp endonuclease activity (type I) towards the 3′-phosphotyrosyl intermediate resulting from strand cleavage. This risk is not alleviated by phosphate electrostatics; neutralizing the negative charge on the scissile phosphate through methylphosphonate (MeP) substitution does not stimulate type I endonuclease. Rather, protection derives from the architecture of the recombination synapse and conformational dynamics within it. Strand cleavage is protected against water by active site electrostatics. Replacement of the catalytic Arg-308 of Flp by alanine, along with MeP substitution, elicits a second Flp endonuclease activity (type II) that directly targets the scissile phosphodiester bond in DNA. MeP substitution, combined with appropriate active site mutations, will be useful in revealing anti-hydrolytic mechanisms engendered by systems that mediate DNA relaxation, DNA transposition, site-specific recombination, telomere resolution, RNA splicing and retrohoming of mobile introns. PMID:19440204

Architectural freedom and industrialized architecture

DEFF Research Database (Denmark)

Vestergaard, Inge

2012-01-01

to explain that architecture can be thought as a complex and diverse design through customization, telling exactly the revitalized storey about the change to a contemporary sustainable and better performing expression in direct relation to the given context. Through the last couple of years we have...... proportions, to organize the process on site choosing either one room wall components or several rooms wall components – either horizontally or vertically. Combined with the seamless joint the playing with these possibilities the new industrialized architecture can deliver variations in choice of solutions...... for retrofit design. If we add the question of the installations e.g. ventilation to this systematic thinking of building technique we get a diverse and functional architecture, thereby creating a new and clearer story telling about new and smart system based thinking behind architectural expression....

Architecture of human mTOR complex 1.

Science.gov (United States)

Aylett, Christopher H S; Sauer, Evelyn; Imseng, Stefan; Boehringer, Daniel; Hall, Michael N; Ban, Nenad; Maier, Timm

2016-01-01

Target of rapamycin (TOR), a conserved protein kinase and central controller of cell growth, functions in two structurally and functionally distinct complexes: TORC1 and TORC2. Dysregulation of mammalian TOR (mTOR) signaling is implicated in pathologies that include diabetes, cancer, and neurodegeneration. We resolved the architecture of human mTORC1 (mTOR with subunits Raptor and mLST8) bound to FK506 binding protein (FKBP)-rapamycin, by combining cryo-electron microscopy at 5.9 angstrom resolution with crystallographic studies of Chaetomium thermophilum Raptor at 4.3 angstrom resolution. The structure explains how FKBP-rapamycin and architectural elements of mTORC1 limit access to the recessed active site. Consistent with a role in substrate recognition and delivery, the conserved amino-terminal domain of Raptor is juxtaposed to the kinase active site. Copyright © 2016, American Association for the Advancement of Science.

A New Information Architecture, Web Site and Services for the CMS Experiment

CERN Multimedia

CERN. Geneva

2012-01-01

The age and size of the CMS collaboration at the LHC means it now has many hundreds of inhomogeneous web sites and services and more than 100,000 documents. We describe a major initiative to create a single coherent CMS internal and public web site. This uses the Drupal web Content Management System (now supported by CERN/IT) on top of a standard LAMP stack (Linux, Apache, MySQL, and php/perl). The new navigation, content and search services are coherently integrated with numerous existing CERN services (CDS, EDMS, Indico, phonebook, Twiki) as well as many CMS internal Web services. We describe the information architecture; the system design, implementation and monitoring; the document and content database; security aspects; and our deployment strategy which ensured continual smooth operation of all systems at all times.

Operations Assessment of Launch Vehicle Architectures using Activity Based Cost Models

Science.gov (United States)

Ruiz-Torres, Alex J.; McCleskey, Carey

2000-01-01

The growing emphasis on affordability for space transportation systems requires the assessment of new space vehicles for all life cycle activities, from design and development, through manufacturing and operations. This paper addresses the operational assessment of launch vehicles, focusing on modeling the ground support requirements of a vehicle architecture, and estimating the resulting costs and flight rate. This paper proposes the use of Activity Based Costing (ABC) modeling for this assessment. The model uses expert knowledge to determine the activities, the activity times and the activity costs based on vehicle design characteristics. The approach provides several advantages to current approaches to vehicle architecture assessment including easier validation and allowing vehicle designers to understand the cost and cycle time drivers.

Transcriptional decomposition reveals active chromatin architectures and cell specific regulatory interactions

DEFF Research Database (Denmark)

Rennie, Sarah; Dalby, Maria; van Duin, Lucas

2018-01-01

Transcriptional regulation is tightly coupled with chromosomal positioning and three-dimensional chromatin architecture. However, it is unclear what proportion of transcriptional activity is reflecting such organisation, how much can be informed by RNA expression alone and how this impacts disease...... proportion of total levels and is highly informative of topological associating domain activities and organisation, revealing boundaries and chromatin compartments. Furthermore, expression data alone accurately predict individual enhancer-promoter interactions, drawing features from expression strength...... between transcription and chromatin architecture....

On-site and off-site activities

International Nuclear Information System (INIS)

Martin, H.D.

1986-01-01

Design principles for NPP training programs. Effects of NPP contracts. Effects of domestic industrial activities. The role of international bodies. Requirements for on-site training. Training abroad, technical, financial and social aspects. Training center on-site, an evaluation. (orig.)

Genome-wide screens for in vivo Tinman binding sites identify cardiac enhancers with diverse functional architectures.

Directory of Open Access Journals (Sweden)

Hong Jin

Full Text Available The NK homeodomain factor Tinman is a crucial regulator of early mesoderm patterning and, together with the GATA factor Pannier and the Dorsocross T-box factors, serves as one of the key cardiogenic factors during specification and differentiation of heart cells. Although the basic framework of regulatory interactions driving heart development has been worked out, only about a dozen genes involved in heart development have been designated as direct Tinman target genes to date, and detailed information about the functional architectures of their cardiac enhancers is lacking. We have used immunoprecipitation of chromatin (ChIP from embryos at two different stages of early cardiogenesis to obtain a global overview of the sequences bound by Tinman in vivo and their linked genes. Our data from the analysis of ~50 sequences with high Tinman occupancy show that the majority of such sequences act as enhancers in various mesodermal tissues in which Tinman is active. All of the dorsal mesodermal and cardiac enhancers, but not some of the others, require tinman function. The cardiac enhancers feature diverse arrangements of binding motifs for Tinman, Pannier, and Dorsocross. By employing these cardiac and non-cardiac enhancers in machine learning approaches, we identify a novel motif, termed CEE, as a classifier for cardiac enhancers. In vivo assays for the requirement of the binding motifs of Tinman, Pannier, and Dorsocross, as well as the CEE motifs in a set of cardiac enhancers, show that the Tinman sites are essential in all but one of the tested enhancers; although on occasion they can be functionally redundant with Dorsocross sites. The enhancers differ widely with respect to their requirement for Pannier, Dorsocross, and CEE sites, which we ascribe to their different position in the regulatory circuitry, their distinct temporal and spatial activities during cardiogenesis, and functional redundancies among different factor binding sites.

Integrated Characterization of DNAPL Source Zone Architecture in Clay Till and Limestone Bedrock

DEFF Research Database (Denmark)

Broholm, Mette Martina; Janniche, Gry Sander; Fjordbøge, Annika Sidelmann

2014-01-01

Background/Objectives. Characterization of dense non-aqueous phase liquid (DNAPL) source zone architecture is essential to develop accurate site specific conceptual models, delineate and quantify contaminant mass, perform risk assessment, and select and design remediation alternatives. The activi......Background/Objectives. Characterization of dense non-aqueous phase liquid (DNAPL) source zone architecture is essential to develop accurate site specific conceptual models, delineate and quantify contaminant mass, perform risk assessment, and select and design remediation alternatives...... innovative investigation methods and characterize the source zone hydrogeology and contamination to obtain an improved conceptual understanding of DNAPL source zone architecture in clay till and bryozoan limestone bedrock. Approach/Activities. A wide range of innovative and current site investigative tools...... for direct and indirect documentation and/or evaluation of DNAPL presence were combined in a multiple lines of evidence approach. Results/Lessons Learned. Though no single technique was sufficient for characterization of DNAPL source zone architecture, the combined use of membrane interphase probing (MIP...

A Single-Transistor Active Pixel CMOS Image Sensor Architecture

International Nuclear Information System (INIS)

Zhang Guo-An; He Jin; Zhang Dong-Wei; Su Yan-Mei; Wang Cheng; Chen Qin; Liang Hai-Lang; Ye Yun

2012-01-01

A single-transistor CMOS active pixel image sensor (1 T CMOS APS) architecture is proposed. By switching the photosensing pinned diode, resetting and selecting can be achieved by diode pull-up and capacitive coupling pull-down of the source follower. Thus, the reset and selected transistors can be removed. In addition, the reset and selected signal lines can be shared to reduce the metal signal line, leading to a very high fill factor. The pixel design and operation principles are discussed in detail. The functionality of the proposed 1T CMOS APS architecture has been experimentally verified using a fabricated chip in a standard 0.35 μm CMOS AMIS technology

The NASA Integrated Information Technology Architecture

Science.gov (United States)

Baldridge, Tim

1997-01-01

This document defines an Information Technology Architecture for the National Aeronautics and Space Administration (NASA), where Information Technology (IT) refers to the hardware, software, standards, protocols and processes that enable the creation, manipulation, storage, organization and sharing of information. An architecture provides an itemization and definition of these IT structures, a view of the relationship of the structures to each other and, most importantly, an accessible view of the whole. It is a fundamental assumption of this document that a useful, interoperable and affordable IT environment is key to the execution of the core NASA scientific and project competencies and business practices. This Architecture represents the highest level system design and guideline for NASA IT related activities and has been created on the authority of the NASA Chief Information Officer (CIO) and will be maintained under the auspices of that office. It addresses all aspects of general purpose, research, administrative and scientific computing and networking throughout the NASA Agency and is applicable to all NASA administrative offices, projects, field centers and remote sites. Through the establishment of five Objectives and six Principles this Architecture provides a blueprint for all NASA IT service providers: civil service, contractor and outsourcer. The most significant of the Objectives and Principles are the commitment to customer-driven IT implementations and the commitment to a simpler, cost-efficient, standards-based, modular IT infrastructure. In order to ensure that the Architecture is presented and defined in the context of the mission, project and business goals of NASA, this Architecture consists of four layers in which each subsequent layer builds on the previous layer. They are: 1) the Business Architecture: the operational functions of the business, or Enterprise, 2) the Systems Architecture: the specific Enterprise activities within the context

DOE site performance assessment activities

International Nuclear Information System (INIS)

1990-07-01

Information on performance assessment capabilities and activities was collected from eight DOE sites. All eight sites either currently dispose of low-level radioactive waste (LLW) or plan to dispose of LLW in the near future. A survey questionnaire was developed and sent to key individuals involved in DOE Order 5820.2A performance assessment activities at each site. The sites surveyed included: Hanford Site (Hanford), Idaho National Engineering Laboratory (INEL), Los Alamos National Laboratory (LANL), Nevada Test Site (NTS), Oak Ridge National Laboratory (ORNL), Paducah Gaseous Diffusion Plant (Paducah), Portsmouth Gaseous Diffusion Plant (Portsmouth), and Savannah River Site (SRS). The questionnaire addressed all aspects of the performance assessment process; from waste source term to dose conversion factors. This report presents the information developed from the site questionnaire and provides a comparison of site-specific performance assessment approaches, data needs, and ongoing and planned activities. All sites are engaged in completing the radioactive waste disposal facility performance assessment required by DOE Order 5820.2A. Each site has achieved various degrees of progress and have identified a set of critical needs. Within several areas, however, the sites identified common needs and questions

Embedded active vision system based on an FPGA architecture

OpenAIRE

Chalimbaud , Pierre; Berry , François

2006-01-01

International audience; In computer vision and more particularly in vision processing, the impressive evolution of algorithms and the emergence of new techniques dramatically increase algorithm complexity. In this paper, a novel FPGA-based architecture dedicated to active vision (and more precisely early vision) is proposed. Active vision appears as an alternative approach to deal with artificial vision problems. The central idea is to take into account the perceptual aspects of visual tasks,...

Active materials for adaptive architectural envelopes based on plant adaptation principles

Directory of Open Access Journals (Sweden)

Marlen Lopez

2015-06-01

Full Text Available In this paper, the authors present research into adaptive architectural envelopes that adapt to environmental changes using active materials, as a result of application of biomimetic principles from plants to architecture. Buildings use large amounts of energy in order to maintain their internal comfort, because conventional buildings are designed to provide a static design solution. Most of the current solutions for facades are not designed for optimum adaptation to contextual issues and needs, while biological solutions to adaptation are often complex, multi-functional and highly responsive. We focus on plant adaptations to the environment, as, due to their immobility, they have developed special means of protection against weather changing conditions. Furthermore, recent developments in new technologies are allowing the possibility to transfer these plant adaptation strategies to technical implementation. These technologies include: multi-material 3D printing, advances in materials science and new capabilities in simulation software. Unlike traditional mechanical activation used for dynamic systems in kinetic facades, adaptive architectural envelopes require no complex electronics, sensors, or actuators. The paper proposes a research of the relationship that can be developed between active materials and environmental issues in order to propose innovative and low-tech design strategies to achieve living envelopes according to plant adaptation principles.  

Hierarchical nitrogen doped bismuth niobate architectures: Controllable synthesis and excellent photocatalytic activity

International Nuclear Information System (INIS)

Hou, Jungang; Cao, Rui; Wang, Zheng; Jiao, Shuqiang; Zhu, Hongmin

2012-01-01

Graphical abstract: Efficient visible-light-driven photocatalysts of peony-like nitrogen doped Bi 3 NbO 7 hierarchical architectures and silver-layered Bi 3 NbO 7−x Nx heterostructures were successfully synthesized in this discovery. Highlights: ► N-Bi 3 NbO 7 architectures were synthesized via two-step hydrothermal process. ► Electronic structure calculations indicated that N replaced O in samples. ► Growth mechanism is proposed for transformation of nanoparticles to microflowers. ► Excellent activities of N-Bi 3 NbO 7 architectures were obtained for degradation. ► Enhanced photocatalytic performance was observed for Ag/N-Bi 3 NbO 7 architectures. - Abstract: Nitrogen doped bismuth niobate (N-Bi 3 NbO 7 ) hierarchical architectures were synthesized via a facile two-step hydrothermal process. XRD patterns revealed that the defect fluorite-type crystal structure of Bi 3 NbO 7 remained intact upon nitrogen doping. Electron microscopy showed the N-Bi 3 NbO 7 architecture has a unique peony-like spherical superstructure composed of numerous nanosheets. UV–vis spectra indicated that nitrogen doping in the compound results in a red-shift of the absorption edge from 450 nm to 470 nm. XPS indicated that [Bi/Nb]-N bonds were formed by inducing nitrogen to replace a small amount of oxygen in Bi 3 NbO 7−x N x , which is explained by electronic structure calculations including energy band and density of states. Based on observations of architectures formation, a possible growth mechanism was proposed to explain the transformation of polyhedral-like nanoparticles to peony-like microflowers via an Ostwald riping mechanism followed by self-assembly. The N-Bi 3 NbO 7 architectures due to the large specific surface area and nitrogen doping exhibited higher photocatalytic activities in the decomposition of organic pollutant under visible-light irradiation than Bi 3 NbO 7 nanoparticles. Furthermore, an enhanced photocatalytic performance was also observed for Ag

A gratuitous β-Lactamase inducer uncovers hidden active site dynamics of the Staphylococcus aureus BlaR1 sensor domain.

Science.gov (United States)

Frederick, Thomas E; Peng, Jeffrey W

2018-01-01

Increasing evidence shows that active sites of proteins have non-trivial conformational dynamics. These dynamics include active site residues sampling different local conformations that allow for multiple, and possibly novel, inhibitor binding poses. Yet, active site dynamics garner only marginal attention in most inhibitor design efforts and exert little influence on synthesis strategies. This is partly because synthesis requires a level of atomic structural detail that is frequently missing in current characterizations of conformational dynamics. In particular, while the identity of the mobile protein residues may be clear, the specific conformations they sample remain obscure. Here, we show how an appropriate choice of ligand can significantly sharpen our abilities to describe the interconverting binding poses (conformations) of protein active sites. Specifically, we show how 2-(2'-carboxyphenyl)-benzoyl-6-aminopenicillanic acid (CBAP) exposes otherwise hidden dynamics of a protein active site that binds β-lactam antibiotics. When CBAP acylates (binds) the active site serine of the β-lactam sensor domain of BlaR1 (BlaRS), it shifts the time scale of the active site dynamics to the slow exchange regime. Slow exchange enables direct characterization of inter-converting protein and bound ligand conformations using NMR methods. These methods include chemical shift analysis, 2-d exchange spectroscopy, off-resonance ROESY of the bound ligand, and reduced spectral density mapping. The active site architecture of BlaRS is shared by many β-lactamases of therapeutic interest, suggesting CBAP could expose functional motions in other β-lactam binding proteins. More broadly, CBAP highlights the utility of identifying chemical probes common to structurally homologous proteins to better expose functional motions of active sites.

A three-dimensional model of mammalian tyrosinase active site accounting for loss of function mutations.

Science.gov (United States)

Schweikardt, Thorsten; Olivares, Concepción; Solano, Francisco; Jaenicke, Elmar; García-Borrón, José Carlos; Decker, Heinz

2007-10-01

Tyrosinases are the first and rate-limiting enzymes in the synthesis of melanin pigments responsible for colouring hair, skin and eyes. Mutation of tyrosinases often decreases melanin production resulting in albinism, but the effects are not always understood at the molecular level. Homology modelling of mouse tyrosinase based on recently published crystal structures of non-mammalian tyrosinases provides an active site model accounting for loss-of-function mutations. According to the model, the copper-binding histidines are located in a helix bundle comprising four densely packed helices. A loop containing residues M374, S375 and V377 connects the CuA and CuB centres, with the peptide oxygens of M374 and V377 serving as hydrogen acceptors for the NH-groups of the imidazole rings of the copper-binding His367 and His180. Therefore, this loop is essential for the stability of the active site architecture. A double substitution (374)MS(375) --> (374)GG(375) or a single M374G mutation lead to a local perturbation of the protein matrix at the active site affecting the orientation of the H367 side chain, that may be unable to bind CuB reliably, resulting in loss of activity. The model also accounts for loss of function in two naturally occurring albino mutations, S380P and V393F. The hydroxyl group in S380 contributes to the correct orientation of M374, and the substitution of V393 for a bulkier phenylalanine sterically impedes correct side chain packing at the active site. Therefore, our model explains the mechanistic necessity for conservation of not only active site histidines but also adjacent amino acids in tyrosinase.

Cultural schema and design activity in an architectural design studio

Directory of Open Access Journals (Sweden)

Gökçe Ketizmen Önal

2017-06-01

Full Text Available Research on the cognitive activities and on the structure and quality of knowledge flow involved in architectural design education is increasing. These studies generally focus on the interaction between student and instructor, including processes such as producing ideas, solving display problems, and integrating design strategies. These studies commonly include computational evaluations and confirmation of the coding of knowledge. They may also include the determination of designer׳s thoughts and cognitive actions of design process, as well as the analysis and digitization of verbal protocols during the design process. In most of these studies, the designer׳s cultural and psychological components are not considered. Accordingly, research on the effects of designers’ cultural schema on design activity in design studios is limited. This study aimed to solve this problem by analyzing the relationship between design activity and the designer׳s cultural schema in a design studio. We performed an experimental study based on a specific conceptual framework and a research model aimed at identifying the relationships among cultural schemas, the architectural design process, and design studios.

Efficient universal computing architectures for decoding neural activity.

Directory of Open Access Journals (Sweden)

Benjamin I Rapoport

Full Text Available The ability to decode neural activity into meaningful control signals for prosthetic devices is critical to the development of clinically useful brain- machine interfaces (BMIs. Such systems require input from tens to hundreds of brain-implanted recording electrodes in order to deliver robust and accurate performance; in serving that primary function they should also minimize power dissipation in order to avoid damaging neural tissue; and they should transmit data wirelessly in order to minimize the risk of infection associated with chronic, transcutaneous implants. Electronic architectures for brain- machine interfaces must therefore minimize size and power consumption, while maximizing the ability to compress data to be transmitted over limited-bandwidth wireless channels. Here we present a system of extremely low computational complexity, designed for real-time decoding of neural signals, and suited for highly scalable implantable systems. Our programmable architecture is an explicit implementation of a universal computing machine emulating the dynamics of a network of integrate-and-fire neurons; it requires no arithmetic operations except for counting, and decodes neural signals using only computationally inexpensive logic operations. The simplicity of this architecture does not compromise its ability to compress raw neural data by factors greater than [Formula: see text]. We describe a set of decoding algorithms based on this computational architecture, one designed to operate within an implanted system, minimizing its power consumption and data transmission bandwidth; and a complementary set of algorithms for learning, programming the decoder, and postprocessing the decoded output, designed to operate in an external, nonimplanted unit. The implementation of the implantable portion is estimated to require fewer than 5000 operations per second. A proof-of-concept, 32-channel field-programmable gate array (FPGA implementation of this portion

Intelligent Architecture for Enhanced Observability for Active Distribution System

DEFF Research Database (Denmark)

Pokhrel, Basanta Raj; Nainar, Karthikeyan; Bak-Jensen, Birgitte

2017-01-01

There is a rapid increase of renewable energy resources (RE) and demand response resources (DRR) in the distribution networks. This is challenging for the reliable and stable operation of the grid. So, to ensure secure, optimized and economical operation in such active distribution grids they need...... for active distribution network which satisfies the need for higher observability reach with less field observation. Improved state estimation with composite load forecasting model is aimed for enhanced observability. This paper also summarizes the application of intelligent architecture in the operation...

Site organization and site arrangement

International Nuclear Information System (INIS)

Boissonnet, B.; Macqueron, J.F.

1976-01-01

The present paper deals with criteria for the choice of a production unit or power plant site, the organization and development of a site in terms of its particular characteristics and takes into account personnel considerations in site organizations as well as the problem of integrating the architecture into the environment. (RW) [de

ATLAS off-Grid sites (Tier 3) monitoring. From local fabric monitoring to global overview of the VO computing activities

CERN Document Server

PETROSYAN, A; The ATLAS collaboration; BELOV, S; ANDREEVA, J; KADOCHNIKOV, I

2012-01-01

The ATLAS Distributed Computing activities have so far concentrated in the "central" part of the experiment computing system, namely the first 3 tiers (the CERN Tier0, 10 Tier1 centers and over 60 Tier2 sites). Many ATLAS Institutes and National Communities have deployed (or intend to) deploy Tier-3 facilities. Tier-3 centers consist of non-pledged resources, which are usually dedicated to data analysis tasks by the geographically close or local scientific groups, and which usually comprise a range of architectures without Grid middleware. Therefore a substantial part of the ATLAS monitoring tools which make use of Grid middleware, cannot be used for a large fraction of Tier3 sites. The presentation will describe the T3mon project, which aims to develop a software suite for monitoring the Tier3 sites, both from the perspective of the local site administrator and that of the ATLAS VO, thereby enabling the global view of the contribution from Tier3 sites to the ATLAS computing activities. Special attention in p...

Polyhomologation based on in situ generated Boron-thexyl-silaboracyclic initiating sites: a novel strategy towards the synthesis of polyethylene-based complex architectures

KAUST Repository

Zhang, Zhen; Zhang, Hefeng; Gnanou, Yves; Hadjichristidis, Nikolaos

2015-01-01

A novel strategy, based on the in situ generated Boron-thexyl-silaboracyclic initiating sites for the polyhomologation of dimethylsulfoxonium methylide, has been developed for the synthesis of complex polyethylene-based architectures. As examples

Physical Activity Design Guidelines for School Architecture.

Science.gov (United States)

Brittin, Jeri; Sorensen, Dina; Trowbridge, Matthew; Lee, Karen K; Breithecker, Dieter; Frerichs, Leah; Huang, Terry

2015-01-01

Increasing children's physical activity at school is a national focus in the U.S. to address childhood obesity. While research has demonstrated associations between aspects of school environments and students' physical activity, the literature currently lacks a synthesis of evidence to serve as a practical, spatially-organized resource for school designers and decision-makers, as well as to point to pertinent research opportunities. This paper describes the development of a new practical tool: Physical Activity Design Guidelines for School Architecture. Its aims are to provide architects and designers, as well as school planners, educators, and public health professionals, with strategies for making K-12 school environments conducive to healthy physical activity, and to engage scientists in transdisciplinary perspectives toward improved knowledge of the school environment's impact. We used a qualitative review process to develop evidence-based and theory-driven school design guidelines that promote increased physical activity among students. The design guidelines include specific strategies in 10 school design domains. Implementation of the guidelines is expected to enable students to adopt healthier physical activity behaviors. The tool bridges a translational gap between research and environmental design practice, and may contribute to setting new industry and education standards.

Architectural Engineers

DEFF Research Database (Denmark)

Petersen, Rikke Premer

engineering is addresses from two perspectives – as an educational response and an occupational constellation. Architecture and engineering are two of the traditional design professions and they frequently meet in the occupational setting, but at educational institutions they remain largely estranged....... The paper builds on a multi-sited study of an architectural engineering program at the Technical University of Denmark and an architectural engineering team within an international engineering consultancy based on Denmark. They are both responding to new tendencies within the building industry where...... the role of engineers and architects increasingly overlap during the design process, but their approaches reflect different perceptions of the consequences. The paper discusses some of the challenges that design education, not only within engineering, is facing today: young designers must be equipped...

Coral identity underpins architectural complexity on Caribbean reefs.

Science.gov (United States)

Alvarez-Filip, Lorenzo; Dulvy, Nicholas K; Côte, Isabelle M; Watkinson, Andrew R; Gill, Jennifer A

2011-09-01

The architectural complexity of ecosystems can greatly influence their capacity to support biodiversity and deliver ecosystem services. Understanding the components underlying this complexity can aid the development of effective strategies for ecosystem conservation. Caribbean coral reefs support and protect millions of livelihoods, but recent anthropogenic change is shifting communities toward reefs dominated by stress-resistant coral species, which are often less architecturally complex. With the regionwide decline in reef fish abundance, it is becoming increasingly important to understand changes in coral reef community structure and function. We quantify the influence of coral composition, diversity, and morpho-functional traits on the architectural complexity of reefs across 91 sites at Cozumel, Mexico. Although reef architectural complexity increases with coral cover and species richness, it is highest on sites that are low in taxonomic evenness and dominated by morpho-functionally important, reef-building coral genera, particularly Montastraea. Sites with similar coral community composition also tend to occur on reefs with very similar architectural complexity, suggesting that reef structure tends to be determined by the same key species across sites. Our findings provide support for prioritizing and protecting particular reef types, especially those dominated by key reef-building corals, in order to enhance reef complexity.

Structural changes in human cytomegalovirus cytoplasmic assembly sites in the absence of UL97 kinase activity

International Nuclear Information System (INIS)

Azzeh, Maysa; Honigman, Alik; Taraboulos, Albert; Rouvinski, Alexander; Wolf, Dana G.

2006-01-01

Studies of human cytomegalovirus (HCMV) UL97 kinase deletion mutant (ΔUL97) indicated a multi-step role for this kinase in early and late phases of the viral life cycle, namely, in DNA replication, capsid maturation and nuclear egress. Here, we addressed its possible involvement in cytoplasmic steps of HCMV assembly. Using the ΔUL97 and the UL97 kinase inhibitor NGIC-I, we demonstrate that the absence of UL97 kinase activity results in a modified subcellular distribution of the viral structural protein assembly sites, from compact structures impacting upon the nucleus to diffuse perinuclear structures punctuated by large vacuoles. Infection by either wild type or ΔUL97 viruses induced a profound reorganization of wheat germ agglutinin (WGA)-positive Golgi-related structures. Importantly, the viral-induced Golgi remodeling along with the reorganization of the nuclear architecture was substantially altered in the absence of UL97 kinase activity. These findings suggest that UL97 kinase activity might contribute to organization of the viral cytoplasmic assembly sites

Utility experiences in redevelopment of formerly used sites -- Wisconsin Electric's risk management and economic development activities

International Nuclear Information System (INIS)

Borofka, B.P.

1999-01-01

Wisconsin Electric Power Company, which recently celebrated its 100th anniversary, has actively promoted the redevelopment of its former sites as well as those of its customers. Serving Milwaukee and southeast Wisconsin, Wisconsin Electric's (WE) sites include former power plants, landfills, right-of-ways, and manufactured gas plant sites. In setting an example for others, as well as seeking to maximize the economic value of these sites, WE has either redeveloped or promoted the redevelopment of these sites by others. Examples include the East Wells Power Plant (now home of the Milwaukee Repertory Theater), the Lakeside Power Plant Site (now the home of Harnischfeger Corporation's headquarters), and the Commerce Street Power Plant located on the Milwaukee River near downtown Milwaukee. In each case the company evaluated the potential environmental liabilities against the unrealized asset value derived from facility location, site size, architectural uniqueness, or other characteristics. At the Commerce Street Power Plant, walking distance to the downtown Milwaukee business district combined with river frontage, were significant site values leveraged against a $5 million asbestos and lead-based paint removal project done to prepare the plant for marketing. More recently, WE has used its experience in promoting the redevelopment of the Menomonee River Valley, the original core of Milwaukee's industrial community, and in advancing a more practical regulatory approach to redeveloping older sites. Finally, the company is working with a non-profit community health clinic, community groups and local foundations in linking these redevelopment activities with the economic and physical health of inner city residents

Lifecycle Prognostics Architecture for Selected High-Cost Active Components

Energy Technology Data Exchange (ETDEWEB)

N. Lybeck; B. Pham; M. Tawfik; J. B. Coble; R. M. Meyer; P. Ramuhalli; L. J. Bond

2011-08-01

There are an extensive body of knowledge and some commercial products available for calculating prognostics, remaining useful life, and damage index parameters. The application of these technologies within the nuclear power community is still in its infancy. Online monitoring and condition-based maintenance is seeing increasing acceptance and deployment, and these activities provide the technological bases for expanding to add predictive/prognostics capabilities. In looking to deploy prognostics there are three key aspects of systems that are presented and discussed: (1) component/system/structure selection, (2) prognostic algorithms, and (3) prognostics architectures. Criteria are presented for component selection: feasibility, failure probability, consequences of failure, and benefits of the prognostics and health management (PHM) system. The basis and methods commonly used for prognostics algorithms are reviewed and summarized. Criteria for evaluating PHM architectures are presented: open, modular architecture; platform independence; graphical user interface for system development and/or results viewing; web enabled tools; scalability; and standards compatibility. Thirteen software products were identified and discussed in the context of being potentially useful for deployment in a PHM program applied to systems in a nuclear power plant (NPP). These products were evaluated by using information available from company websites, product brochures, fact sheets, scholarly publications, and direct communication with vendors. The thirteen products were classified into four groups of software: (1) research tools, (2) PHM system development tools, (3) deployable architectures, and (4) peripheral tools. Eight software tools fell into the deployable architectures category. Of those eight, only two employ all six modules of a full PHM system. Five systems did not offer prognostic estimates, and one system employed the full health monitoring suite but lacked operations and

Lifecycle Prognostics Architecture for Selected High-Cost Active Components

International Nuclear Information System (INIS)

Lybeck, N.; Pham, B.; Tawfik, M.; Coble, J.B.; Meyer, R.M.; Ramuhalli, P.; Bond, L.J.

2011-01-01

There are an extensive body of knowledge and some commercial products available for calculating prognostics, remaining useful life, and damage index parameters. The application of these technologies within the nuclear power community is still in its infancy. Online monitoring and condition-based maintenance is seeing increasing acceptance and deployment, and these activities provide the technological bases for expanding to add predictive/prognostics capabilities. In looking to deploy prognostics there are three key aspects of systems that are presented and discussed: (1) component/system/structure selection, (2) prognostic algorithms, and (3) prognostics architectures. Criteria are presented for component selection: feasibility, failure probability, consequences of failure, and benefits of the prognostics and health management (PHM) system. The basis and methods commonly used for prognostics algorithms are reviewed and summarized. Criteria for evaluating PHM architectures are presented: open, modular architecture; platform independence; graphical user interface for system development and/or results viewing; web enabled tools; scalability; and standards compatibility. Thirteen software products were identified and discussed in the context of being potentially useful for deployment in a PHM program applied to systems in a nuclear power plant (NPP). These products were evaluated by using information available from company websites, product brochures, fact sheets, scholarly publications, and direct communication with vendors. The thirteen products were classified into four groups of software: (1) research tools, (2) PHM system development tools, (3) deployable architectures, and (4) peripheral tools. Eight software tools fell into the deployable architectures category. Of those eight, only two employ all six modules of a full PHM system. Five systems did not offer prognostic estimates, and one system employed the full health monitoring suite but lacked operations and

Performative Architecture and Urban Spaces

DEFF Research Database (Denmark)

Kiib, Hans

2008-01-01

  3 Workshops one exibition   Three conceptual architectural workshops took take place in parallel from August 16th - 22nd 2008. Each workshop carried a specific methodology and the goal is to come up with conceptual proposals that could be further developed for selected sites in the city of Aalb...... This workshop focus on temporary architecture and urban catalysts. Informal spaces and the interface between the built and the void are foremost in the development of performative urban environments and cultural interaction. ......  3 Workshops one exibition   Three conceptual architectural workshops took take place in parallel from August 16th - 22nd 2008. Each workshop carried a specific methodology and the goal is to come up with conceptual proposals that could be further developed for selected sites in the city...... The workshop model includes an open workshop where a handful of international architects are invited to spend five days with local architects, engineers and scholars contributing to a work of architectural vision and quality. The workshop includes presentations and discussions and development of projects...

Roadmap to the SRS computing architecture

Energy Technology Data Exchange (ETDEWEB)

Johnson, A.

1994-07-05

This document outlines the major steps that must be taken by the Savannah River Site (SRS) to migrate the SRS information technology (IT) environment to the new architecture described in the Savannah River Site Computing Architecture. This document proposes an IT environment that is {open_quotes}...standards-based, data-driven, and workstation-oriented, with larger systems being utilized for the delivery of needed information to users in a client-server relationship.{close_quotes} Achieving this vision will require many substantial changes in the computing applications, systems, and supporting infrastructure at the site. This document consists of a set of roadmaps which provide explanations of the necessary changes for IT at the site and describes the milestones that must be completed to finish the migration.

A software architectural framework specification for neutron activation analysis

International Nuclear Information System (INIS)

Preston, J.A.; Grant, C.N.

2013-01-01

Neutron Activation Analysis (NAA) is a sensitive multi-element nuclear analytical technique that has been routinely applied by research reactor (RR) facilities to environmental, nutritional, health related, geological and geochemical studies. As RR facilities face calls to increase their research output and impact, with existing or reducing budgets, automation of NAA offers a possible solution. However, automation has many challenges, not the least of which is a lack of system architecture standards to establish acceptable mechanisms for the various hardware/software and software/software interactions among data acquisition systems, specialised hardware such as sample changers, sample loaders, and data processing modules. This lack of standardization often results in automation hardware and software being incompatible with existing system components, in a facility looking to automate its NAA operations. This limits the availability of automation to a few RR facilities with adequate budgets or in-house engineering resources. What is needed is a modern open system architecture for NAA, that provides the required set of functionalities. This paper describes such an 'architectural framework' (OpenNAA), and portions of a reference implementation. As an example of the benefits, calculations indicate that applying this architecture to the compilation and QA steps associated with the analysis of 35 elements in 140 samples, with 14 SRM's, can reduce the time required by over 80 %. The adoption of open standards in the nuclear industry has been very successful over the years in promoting interchangeability and maximising the lifetime and output of nuclear measurement systems. OpenNAA will provide similar benefits within the NAA application space, safeguarding user investments in their current system, while providing a solid path for development into the future. (author)

Architecture, cobbled roads and chronology of the main sector of the site Las Mercedes-1, Central Caribbean of Costa Rica

International Nuclear Information System (INIS)

Vazquez Leiva, Ricardo; Chapdelaine, Claude

2008-01-01

Results from test-pit excavations, survey, and mapping at Las Mercedes-1, plus the center of a paramount chiefly polity in the Central Caribbean region of Costa Rica, are presented. Information on architecture, construction system, ceramic chronology, and funerary features, are reported. Radiocarbon dates have suggested that important construction works at the site's center were carried out circa A.D. 1000. Two cobble-paved, causeways, perpendicular to the river drainage system, were found to be linked to the main monumental compound as roads for formal access. Interest in the site has been revived and points the way to further researches at the site and its regional sphere. (author) [es

Physical Activity Design Guidelines for School Architecture.

Directory of Open Access Journals (Sweden)

Jeri Brittin

Full Text Available Increasing children's physical activity at school is a national focus in the U.S. to address childhood obesity. While research has demonstrated associations between aspects of school environments and students' physical activity, the literature currently lacks a synthesis of evidence to serve as a practical, spatially-organized resource for school designers and decision-makers, as well as to point to pertinent research opportunities. This paper describes the development of a new practical tool: Physical Activity Design Guidelines for School Architecture. Its aims are to provide architects and designers, as well as school planners, educators, and public health professionals, with strategies for making K-12 school environments conducive to healthy physical activity, and to engage scientists in transdisciplinary perspectives toward improved knowledge of the school environment's impact. We used a qualitative review process to develop evidence-based and theory-driven school design guidelines that promote increased physical activity among students. The design guidelines include specific strategies in 10 school design domains. Implementation of the guidelines is expected to enable students to adopt healthier physical activity behaviors. The tool bridges a translational gap between research and environmental design practice, and may contribute to setting new industry and education standards.

A method for valuing architecture-based business transformation and measuring the value of solutions architecture

OpenAIRE

Slot, R.G.

2010-01-01

Enterprise and Solution Architecture are key in today’s business environment. It is surprising that the foundation and business case for these activities are nonexistent; the financial value for the business of these activities is largely undetermined. To determine business value of enterprise and solution architecture, this thesis shows how to measure and quantify, in business terms, the value of enterprise architecture-based on business transformation and the value of solution architecture.

ECONOMY AND CULTURE IN TRANSITIONS: A COMPARATIVE STUDY OF TWO ARCHITECTURAL HERITAGE SITES OF BAZARS AND HANS OF BURSA AND DHAKA

Directory of Open Access Journals (Sweden)

Tasleem Shakur

2013-11-01

Full Text Available Chawkbazar in Dhaka and Covered Bazaar in Bursa are two unique examples showing the contradiction among the traditional and the contemporary life-styles and their reflections on space uses and architecture. The first example in Dhaka is one of the prime icons of Mughal architecture in Bangladesh, whereas the second one in Bursa is the first example of the typical nucleus of traditional Ottoman city. Based on these two case studies in Dhaka (Bangladesh and Bursa (Turkey, this paper attempts to illustrate how the emerging cultures and spaces are continuously being either negotiated or contested with their adjacent historic sites (Shakur, 2005. This comparative research between two similar historical sites (Mughal and Ottoman but miles apart geographically is intended to understand the commonalities in its economic, social, cultural aspects through its transformation from the historic to the contemporary period. It highlights the socio-economic and cultural transformation and its implications for future conservation and development.

Architecture Descriptions. A Contribution to Modeling of Production System Architecture

DEFF Research Database (Denmark)

Jepsen, Allan Dam; Hvam, Lars

a proper understanding of the architecture phenomenon and the ability to describe it in a manner that allow the architecture to be communicated to and handled by stakeholders throughout the company. Despite the existence of several design philosophies in production system design such as Lean, that focus...... a diverse set of stakeholder domains and tools in the production system life cycle. To support such activities, a contribution is made to the identification and referencing of production system elements within architecture descriptions as part of the reference architecture framework. The contribution...

Control of Architecture in Rhombic Dodecahedral Pt–Ni Nanoframe Electrocatalysts

Energy Technology Data Exchange (ETDEWEB)

Becknell, Nigel [Department; Materials; Son, Yoonkook [Department; Kim, Dohyung [Department; Li, Dongguo [Materials; Yu, Yi [Department; Niu, Zhiqiang [Department; Lei, Teng [Department; Sneed, Brian T. [Center; More, Karren L. [Center; Markovic, Nenad M. [Materials; Stamenkovic, Vojislav R. [Materials; Yang, Peidong [Department; Materials; Department; Kavli Energy NanoSciences Institute, Berkeley, California 94720, United States

2017-08-08

Platinum-based alloys are known to demonstrate advanced properties in electrochemical reactions that are relevant for proton exchange membrane fuel cells and electrolyzers. Further development of Pt alloy electrocatalysts relies on the design of architectures with highly active surfaces and optimized utilization of the expensive elpment, Pt. Here, we show that the three-dimensional Pt anisotropy of Pt-Ni rhombic dodecahedra can be tuned by controlling the ratio between Pt and Ni precursors such that either a completely hollow nanoframe or a new architecture, the excavated nanoframe, can be obtained. The excavated nanoframe showed similar to 10 times higher specific and similar to 6 times higher mass activity for the oxygen reduction reaction than Pt/C, and twice the mass activity of the hollow nanoframe. The high activity is attributed to enhanced Ni content in the near-surface region and the extended two-dimensional sheet structure within the nanoframe that minimizes the number of buried Pt sites.

An SOA-based architecture framework

NARCIS (Netherlands)

Aalst, van der W.M.P.; Beisiegel, M.; Hee, van K.M.; König, D.; Stahl, C.

2007-01-01

We present an Service-Oriented Architecture (SOA)– based architecture framework. The architecture framework is designed to be close to industry standards, especially to the Service Component Architecture (SCA). The framework is language independent and the building blocks of each system, activities

Active site similarity between human and Plasmodium falciparum phosphodiesterases: considerations for antimalarial drug design

Science.gov (United States)

Howard, Brittany L.; Thompson, Philip E.; Manallack, David T.

2011-08-01

The similarity between Plasmodium falciparum phosphodiesterase enzymes ( PfPDEs) and their human counterparts have been examined and human PDE9A was found to be a suitable template for the construction of homology models for each of the four PfPDE isoforms. In contrast, the architecture of the active sites of each model was most similar to human PDE1. Molecular docking was able to model cyclic guanosine monophosphate (cGMP) substrate binding in each case but a docking mode supporting cyclic adenosine monophosphate (cAMP) binding could not be found. Anticipating the potential of PfPDE inhibitors as anti-malarial drugs, a range of reported PDE inhibitors including zaprinast and sildenafil were docked into the model of PfPDEα. The results were consistent with their reported biological activities, and the potential of PDE1/9 inhibitor analogues was also supported by docking.

A reusable multi-agent architecture for active intelligent websites

NARCIS (Netherlands)

Jonker, C.M.; Lam, R.A.; Treur, J.

In this paper a reusable multi-agent architecture for intelligent Websites is presented and illustrated for an electronic department store. The architecture has been designed and implemented using the compositional design method for multi-agent systems DESIRE. The agents within this architecture are

Automatic web site authoring with SiteGuide

NARCIS (Netherlands)

de Boer, V.; Hollink, V.; van Someren, M.W.; Kłopotek, M.A.; Przepiórkowski, A.; Wierzchoń, S.T.; Trojanowski, K.

2009-01-01

An important step in the design process for a web site is to determine which information is to be included and how the information should be organized on the web site’s pages. In this paper we describe ’SiteGuide’, a tool that automatically produces an information architecture for a web site that a

Thermal Space in Architecture

DEFF Research Database (Denmark)

Petersen, Mads Dines

Present research is revolving around the design process and the use of digital applications to support the design process among architects. This work is made in relation to the current discussions about sustainable architecture and the increased focus on energy consumption and the comfort in our...... and understanding of spaces in buildings can change significantly and instead of the creation of frozen geometrical spaces, thermal spaces can be created as it is suggested in meteorological architecture where functions are distributed in relation to temperature gradients. This creates an interesting contrast......-introducing an increased adaptability in the architecture can be a part of re-defining the environmental agenda and re-establish a link between the environment of the site and the environment of the architecture and through that an increased appreciation of the sensuous space here framed in discussions about thermal...

Architecture Studio Learning: Strategy to Achieve Architects Competence

Directory of Open Access Journals (Sweden)

Saifudin Mutaqi Ahmad

2018-01-01

Full Text Available In most Schools of Architecture, Architecture Studio is at the core of the architectural learning process. In the process, students are trained to have the skills of architectonic spaces design based on the study of the site, its function, and its aesthetics. Students are also trained to have awareness and understanding about the impact of their design on the surrounding environment, both physically and socially. Also, students are trained to present their designs in various forms such as visual graphics, verbal narratives, and three dimensional model animations. Indonesian Association of School of Architecture (APTARI Asosiasi Perguruan Tinggi Arsitektur Indonesia and Indonesian Institute of Architects (IAI - Ikatan Arsitek Indonesia has formulated an education Standards, Curriculum, and Achievements of Architect Professional Program to be referred by Ministry of Research, Technology, and Higher Education (KEMENRISTEKDIKTI – Kementerian Riset, Teknologi, danPerguruanTinggi as the guidance for the implementation of Architect Professional Program (PPA - Pendidikan Profesi Arsitek in Indonesia. One of the eight recommendations is the PPA Content Standard which contains the learning for the achievement of IAI Architect Competencies through the recommended study materials. However, the recommended study materials did not indicate the activity of the Architecture Studio learning model (Final Report of APTARI Part II and IAI. Will architect’s competence be achieved if the learning process withoutarchitectural studio learning model? The formulation of the curriculum that is developed independently by the IAI recommends the learning of Architectural Studio as Professional Studio. The size of the SKS is large enough to enable someone who follows the lesson to intensively gain experience in designing the building as a real architectural work. This Architecture Studio learning model is interpreted by PPAr organizer universities with various forms

Launch Vehicle Control Center Architectures

Science.gov (United States)

Watson, Michael D.; Epps, Amy; Woodruff, Van; Vachon, Michael Jacob; Monreal, Julio; Williams, Randall; McLaughlin, Tom

2014-01-01

This analysis is a survey of control center architectures of the NASA Space Launch System (SLS), United Launch Alliance (ULA) Atlas V and Delta IV, and the European Space Agency (ESA) Ariane 5. Each of these control center architectures have similarities in basic structure, and differences in functional distribution of responsibilities for the phases of operations: (a) Launch vehicles in the international community vary greatly in configuration and process; (b) Each launch site has a unique processing flow based on the specific configurations; (c) Launch and flight operations are managed through a set of control centers associated with each launch site, however the flight operations may be a different control center than the launch center; and (d) The engineering support centers are primarily located at the design center with a small engineering support team at the launch site.

Architectural technology

DEFF Research Database (Denmark)

2005-01-01

The booklet offers an overall introduction to the Institute of Architectural Technology and its projects and activities, and an invitation to the reader to contact the institute or the individual researcher for further information. The research, which takes place at the Institute of Architectural...... Technology at the Roayl Danish Academy of Fine Arts, School of Architecture, reflects a spread between strategic, goal-oriented pilot projects, commissioned by a ministry, a fund or a private company, and on the other hand projects which originate from strong personal interests and enthusiasm of individual...

Two-step interrogation then recognition of DNA binding site by Integration Host Factor: an architectural DNA-bending protein.

Science.gov (United States)

Velmurugu, Yogambigai; Vivas, Paula; Connolly, Mitchell; Kuznetsov, Serguei V; Rice, Phoebe A; Ansari, Anjum

2018-02-28

The dynamics and mechanism of how site-specific DNA-bending proteins initially interrogate potential binding sites prior to recognition have remained elusive for most systems. Here we present these dynamics for Integration Host factor (IHF), a nucleoid-associated architectural protein, using a μs-resolved T-jump approach. Our studies show two distinct DNA-bending steps during site recognition by IHF. While the faster (∼100 μs) step is unaffected by changes in DNA or protein sequence that alter affinity by >100-fold, the slower (1-10 ms) step is accelerated ∼5-fold when mismatches are introduced at DNA sites that are sharply kinked in the specific complex. The amplitudes of the fast phase increase when the specific complex is destabilized and decrease with increasing [salt], which increases specificity. Taken together, these results indicate that the fast phase is non-specific DNA bending while the slow phase, which responds only to changes in DNA flexibility at the kink sites, is specific DNA kinking during site recognition. Notably, the timescales for the fast phase overlap with one-dimensional diffusion times measured for several proteins on DNA, suggesting that these dynamics reflect partial DNA bending during interrogation of potential binding sites by IHF as it scans DNA.

Neonatal GLP1R activation limits adult adiposity by durably altering hypothalamic architecture

Directory of Open Access Journals (Sweden)

Andrea V. Rozo

2017-07-01

Conclusion: These observations suggest that the acute activation of GLP1R in neonates durably alters hypothalamic architecture to limit adult weight gain and adiposity, identifying GLP1R as a therapeutic target for obesity prevention.

Efficient oxygen electrocatalysis on special active sites

DEFF Research Database (Denmark)

Halck, Niels Bendtsen

throughout this thesis to understand these local structure effects and their influence on surface reactions. The concept of these special active sites is used to explain how oxygen evolution reaction (OER) catalysts can have activities beyond the limits of what was previously thought possible. The concept...... stored in these bonds in an eco-friendly fashion in fuel cells. This thesis explores catalysts for oxygen electrocatalysis and how carefully designed local structures on catalysts surfaces termed special active sites can influence the activity. Density functional theory has been used as a method...... is used to explain the increase in activity observed for the OER catalyst ruthenium dioxide when it is mixed with nickel or cobalt. Manganese and cobalt oxides when in the vicinity of gold also display an increase in OER activity which can be explained by locally created special active sites. Density...

Parametric Approach to Assessing Performance of High-Lift Device Active Flow Control Architectures

Directory of Open Access Journals (Sweden)

Yu Cai

2017-02-01

Full Text Available Active Flow Control is at present an area of considerable research, with multiple potential aircraft applications. While the majority of research has focused on the performance of the actuators themselves, a system-level perspective is necessary to assess the viability of proposed solutions. This paper demonstrates such an approach, in which major system components are sized based on system flow and redundancy considerations, with the impacts linked directly to the mission performance of the aircraft. Considering the case of a large twin-aisle aircraft, four distinct active flow control architectures that facilitate the simplification of the high-lift mechanism are investigated using the demonstrated approach. The analysis indicates a very strong influence of system total mass flow requirement on architecture performance, both for a typical mission and also over the entire payload-range envelope of the aircraft.

Neutron-activation analysis of wall soils of ancient architectural monuments

International Nuclear Information System (INIS)

Khatamov, Sh.; Zhumamuratov, A.; Ibragimov, T.; Tillyaev, T.; Osinskaya, N.S.; Rakhmanova, T.P.; Pulatov, D.D.

2001-01-01

The simplified, relatively inexpensive, and productive multielemental neutron activation techniques for analysis of solid of the architectural monuments of Karakalpakstan have been elaborated. A comparison of the elemental composition of the wall soils of the ancient buildings, constructed at different historical periods, with the composition of the agricultural soils allows us to estimate the present ecological and agrogeochemical states of the agricultural soils and to trace changing the dynamics of about 30 chemical elements. (author)

Nonlinear pattern formation in bone growth and architecture

Directory of Open Access Journals (Sweden)

Phil eSalmon

2015-01-01

Full Text Available The 3D morphology of bone arises through adaptation to its required engineering performance. Genetically and adaptively bone travels along a complex spatio-temporal trajectory to acquire optimal architecture. On a cellular, micro-anatomical scale, what mechanisms coordinate the activity of osteoblasts and osteoclasts to produce complex and efficient bone architectures? One mechanism is examined here – chaotic nonlinear pattern formation (NPF – which underlies in a unifying way natural structures as disparate as trabecular bone, swarms of birds flying, island formation, fluid turbulence and others. At the heart of NPF is the fact that simple rules operating between interacting elements, and Turing-like interaction between global and local signals, lead to complex and structured patterns. The study of group intelligence exhibited by swarming birds or shoaling fish has led to an embodiment of NPF called particle swarm optimization (PSO. This theoretical model could be applicable to the behavior of osteoblasts osteoclasts and osteocytes, seeing them operating socially in response simultaneously to both global and local signals (endocrine, cytokine, mechanical resulting in their clustered activity at formation and resorption sites. This represents problem-solving by social intelligence, and could potentially add further realism to in-silico simulation of bone modeling.What insights has NPF provided to bone biology? One example concerns the genetic disorder Juvenile Pagets Disease (JPD or Idiopathic Hyperphosphatasia, where the anomalous parallel trabecular architecture characteristic of this pathology is consistent with an NPF paradigm by analogy with known experimental NPF systems. Here coupling or feedback between osteoblasts and osteoclasts is the critical element.This NPF paradigm implies a profound link between bone regulation and its architecture: in bone the architecture is the regulation. The former is the emergent consequence of the

New Course Design: Classification Schemes and Information Architecture.

Science.gov (United States)

Weinberg, Bella Hass

2002-01-01

Describes a course developed at St. John's University (New York) in the Division of Library and Information Science that relates traditional classification schemes to information architecture and Web sites. Highlights include functional aspects of information architecture, that is, the way content is structured; assignments; student reactions; and…

Sustainable Architecture in the Context of Regional Activities

Science.gov (United States)

Sołkeiewicz-Kos, Nina

2017-10-01

The relationship between man and the surrounding cultural environment directs attention in urban and architectural design to the realm of interdisciplinary research. As a result, they should create architectural and urban solutions which provide aesthetic satisfaction. They should also generate social bonds, a sense of identity and maintain the specificity of the local building environment, where tradition and the context of surroundings is the starting point for creating a sustainable living environment. Presented problems focus on the analysis of formal, functional and spatial solutions, in which materials and technology were selected in an optimal way. The continuation of the subject concerns the relationship between the use of the local urban, architectural, material and technological solutions and the quality of the cultural space that meets the principles of sustainable development. Adaptation and transformation of old techniques and traditional materials to create contemporary designs is one of the forms of experimentation encountered in contemporary architecture. Its economic, social and ecological aspects are realised in the form of: satisfying the needs of the local community, renewal and maintenance of modern standards of the surrounding buildings, use of local materials and available space. This means striving to design and transform the space already in use, while reducing the impact on the environment. Analysed buildings and urban spaces are an attempt to answer: whether the strategies applied in the field of architectural, technological and material solutions provide the identification of the place and meet the users’ expectations?

Polyhomologation based on in situ generated Boron-thexyl-silaboracyclic initiating sites: a novel strategy towards the synthesis of polyethylene-based complex architectures

KAUST Repository

Zhang, Zhen

2015-04-10

A novel strategy, based on the in situ generated Boron-thexyl-silaboracyclic initiating sites for the polyhomologation of dimethylsulfoxonium methylide, has been developed for the synthesis of complex polyethylene-based architectures. As examples, the synthesis of a 4-arm polyethylene star, three (polystyrene)(polyethylene)2 3-miktoarm stars and a PE-branched double graft copolymers are given.

System architectures for telerobotic research

Science.gov (United States)

Harrison, F. Wallace

1989-01-01

Several activities are performed related to the definition and creation of telerobotic systems. The effort and investment required to create architectures for these complex systems can be enormous; however, the magnitude of process can be reduced if structured design techniques are applied. A number of informal methodologies supporting certain aspects of the design process are available. More recently, prototypes of integrated tools supporting all phases of system design from requirements analysis to code generation and hardware layout have begun to appear. Activities related to system architecture of telerobots are described, including current activities which are designed to provide a methodology for the comparison and quantitative analysis of alternative system architectures.

INFORMATION SYSTEM STRATEGIC PLANNING WITH ENTERPRISE ARCHITECTURE PLANNING

Directory of Open Access Journals (Sweden)

Lola Yorita Astri

2013-05-01

Full Text Available An integrated information system is needed in an enterprise to support businessprocesses run by an enterprise. Therefore, to develop information system can use enterprisearchitecture approach which can define strategic planning of enterprise information system. SMPNegeri 1 Jambi can be viewed as an enterprise because there are entities that should be managedthrough an integrated information system. Since there has been no unification of different elementsin a unity yet, enterprise architecture model using Enterprise Architecture Planning (EAP isneeded which will obtain strategic planning of enterprise information system in SMP Negeri 1Jambi. The goal of strategic planning of information system with Enterprise Architecture Planning(EAP is to define primary activities run by SMP Negeri 1 Jambi and support activities supportingprimary activities. They can be used as a basis for making data architecture which is the entities ofapplication architecture. At last, technology architecture is designed to describe technology neededto provide environment for data application. The plan of implementation is the activity plan madeto implemented architectures by enterprise.

Safety Oversight of Decommissioning Activities at DOE Nuclear Sites

International Nuclear Information System (INIS)

Zull, Lawrence M.; Yeniscavich, William

2008-01-01

The Defense Nuclear Facilities Safety Board (Board) is an independent federal agency established by Congress in 1988 to provide nuclear safety oversight of activities at U.S. Department of Energy (DOE) defense nuclear facilities. The activities under the Board's jurisdiction include the design, construction, startup, operation, and decommissioning of defense nuclear facilities at DOE sites. This paper reviews the Board's safety oversight of decommissioning activities at DOE sites, identifies the safety problems observed, and discusses Board initiatives to improve the safety of decommissioning activities at DOE sites. The decommissioning of former defense nuclear facilities has reduced the risk of radioactive material contamination and exposure to the public and site workers. In general, efforts to perform decommissioning work at DOE defense nuclear sites have been successful, and contractors performing decommissioning work have a good safety record. Decommissioning activities have recently been completed at sites identified for closure, including the Rocky Flats Environmental Technology Site, the Fernald Closure Project, and the Miamisburg Closure Project (the Mound site). The Rocky Flats and Fernald sites, which produced plutonium parts and uranium materials for defense needs (respectively), have been turned into wildlife refuges. The Mound site, which performed R and D activities on nuclear materials, has been converted into an industrial and technology park called the Mound Advanced Technology Center. The DOE Office of Legacy Management is responsible for the long term stewardship of these former EM sites. The Board has reviewed many decommissioning activities, and noted that there are valuable lessons learned that can benefit both DOE and the contractor. As part of its ongoing safety oversight responsibilities, the Board and its staff will continue to review the safety of DOE and contractor decommissioning activities at DOE defense nuclear sites

Embedded Active Vision System Based on an FPGA Architecture

Directory of Open Access Journals (Sweden)

Chalimbaud Pierre

2007-01-01

Full Text Available In computer vision and more particularly in vision processing, the impressive evolution of algorithms and the emergence of new techniques dramatically increase algorithm complexity. In this paper, a novel FPGA-based architecture dedicated to active vision (and more precisely early vision is proposed. Active vision appears as an alternative approach to deal with artificial vision problems. The central idea is to take into account the perceptual aspects of visual tasks, inspired by biological vision systems. For this reason, we propose an original approach based on a system on programmable chip implemented in an FPGA connected to a CMOS imager and an inertial set. With such a structure based on reprogrammable devices, this system admits a high degree of versatility and allows the implementation of parallel image processing algorithms.

Embedded Active Vision System Based on an FPGA Architecture

Directory of Open Access Journals (Sweden)

Pierre Chalimbaud

2006-12-01

Full Text Available In computer vision and more particularly in vision processing, the impressive evolution of algorithms and the emergence of new techniques dramatically increase algorithm complexity. In this paper, a novel FPGA-based architecture dedicated to active vision (and more precisely early vision is proposed. Active vision appears as an alternative approach to deal with artificial vision problems. The central idea is to take into account the perceptual aspects of visual tasks, inspired by biological vision systems. For this reason, we propose an original approach based on a system on programmable chip implemented in an FPGA connected to a CMOS imager and an inertial set. With such a structure based on reprogrammable devices, this system admits a high degree of versatility and allows the implementation of parallel image processing algorithms.

Dashboard applications to monitor experiment activities at sites

Energy Technology Data Exchange (ETDEWEB)

Andreeva, Julia; Gaidioz, Benjamin; Grigoras, Costin; Kokoszkiewicz, Lukasz; Lanciotti, Elisa; Rocha, Ricardo; Saiz, Pablo; Santinelli, Roberto; Sidorova, Irina; Sciaba, Andrea [CERN, European Organization for Nuclear Research (Switzerland); Belforte, Stefano [INFN Trieste (Italy); Boehm, Max [EDS, an HP Company, Plano, TX (United States); Casajus, Adrian [Universitat de Barcelona (Spain); Flix, Josep [PIC, Port d' Informacio CientIfica, Bellaterra (Spain); Tsaregorodtsev, Andrei, E-mail: Elisa.Lanciotti@cern.c, E-mail: Pablo.Saiz@cern.c [CPPM Marseille (France)

2010-04-01

In the framework of a distributed computing environment, such as WLCG, monitoring has a key role in order to keep under control activities going on in sites located in different countries and involving people based in many different sites. To be able to cope with such a large scale heterogeneous infrastructure, it is necessary to have monitoring tools providing a complete and reliable view of the overall performance of the sites. Moreover, the structure of a monitoring system critically depends on the object to monitor and on the users it is addressed to. In this article we will describe two different monitoring systems both aimed to monitor activities and services provided in the WLCG framework, but designed in order to meet the requirements of different users: Site Status Board has an overall view of the services available in all the sites supporting an experiment, whereas Siteview provides a complete view of all the activities going on at a site, for all the experiments supported by the site.

Dashboard applications to monitor experiment activities at sites

International Nuclear Information System (INIS)

Andreeva, Julia; Gaidioz, Benjamin; Grigoras, Costin; Kokoszkiewicz, Lukasz; Lanciotti, Elisa; Rocha, Ricardo; Saiz, Pablo; Santinelli, Roberto; Sidorova, Irina; Sciaba, Andrea; Belforte, Stefano; Boehm, Max; Casajus, Adrian; Flix, Josep; Tsaregorodtsev, Andrei

2010-01-01

In the framework of a distributed computing environment, such as WLCG, monitoring has a key role in order to keep under control activities going on in sites located in different countries and involving people based in many different sites. To be able to cope with such a large scale heterogeneous infrastructure, it is necessary to have monitoring tools providing a complete and reliable view of the overall performance of the sites. Moreover, the structure of a monitoring system critically depends on the object to monitor and on the users it is addressed to. In this article we will describe two different monitoring systems both aimed to monitor activities and services provided in the WLCG framework, but designed in order to meet the requirements of different users: Site Status Board has an overall view of the services available in all the sites supporting an experiment, whereas Siteview provides a complete view of all the activities going on at a site, for all the experiments supported by the site.

Lunar Navigation Architecture Design Considerations

Science.gov (United States)

D'Souza, Christopher; Getchius, Joel; Holt, Greg; Moreau, Michael

2009-01-01

The NASA Constellation Program is aiming to establish a long-term presence on the lunar surface. The Constellation elements (Orion, Altair, Earth Departure Stage, and Ares launch vehicles) will require a lunar navigation architecture for navigation state updates during lunar-class missions. Orion in particular has baselined earth-based ground direct tracking as the primary source for much of its absolute navigation needs. However, due to the uncertainty in the lunar navigation architecture, the Orion program has had to make certain assumptions on the capabilities of such architectures in order to adequately scale the vehicle design trade space. The following paper outlines lunar navigation requirements, the Orion program assumptions, and the impacts of these assumptions to the lunar navigation architecture design. The selection of potential sites was based upon geometric baselines, logistical feasibility, redundancy, and abort support capability. Simulated navigation covariances mapped to entry interface flightpath- angle uncertainties were used to evaluate knowledge errors. A minimum ground station architecture was identified consisting of Goldstone, Madrid, Canberra, Santiago, Hartebeeshoek, Dongora, Hawaii, Guam, and Ascension Island (or the geometric equivalent).

Unified Monitoring Architecture for IT and Grid Services

Science.gov (United States)

Aimar, A.; Aguado Corman, A.; Andrade, P.; Belov, S.; Delgado Fernandez, J.; Garrido Bear, B.; Georgiou, M.; Karavakis, E.; Magnoni, L.; Rama Ballesteros, R.; Riahi, H.; Rodriguez Martinez, J.; Saiz, P.; Zolnai, D.

2017-10-01

This paper provides a detailed overview of the Unified Monitoring Architecture (UMA) that aims at merging the monitoring of the CERN IT data centres and the WLCG monitoring using common and widely-adopted open source technologies such as Flume, Elasticsearch, Hadoop, Spark, Kibana, Grafana and Zeppelin. It provides insights and details on the lessons learned, explaining the work performed in order to monitor the CERN IT data centres and the WLCG computing activities such as the job processing, data access and transfers, and the status of sites and services.

Non-linear pattern formation in bone growth and architecture.

Science.gov (United States)

Salmon, Phil

2014-01-01

The three-dimensional morphology of bone arises through adaptation to its required engineering performance. Genetically and adaptively bone travels along a complex spatiotemporal trajectory to acquire optimal architecture. On a cellular, micro-anatomical scale, what mechanisms coordinate the activity of osteoblasts and osteoclasts to produce complex and efficient bone architectures? One mechanism is examined here - chaotic non-linear pattern formation (NPF) - which underlies in a unifying way natural structures as disparate as trabecular bone, swarms of birds flying, island formation, fluid turbulence, and others. At the heart of NPF is the fact that simple rules operating between interacting elements, and Turing-like interaction between global and local signals, lead to complex and structured patterns. The study of "group intelligence" exhibited by swarming birds or shoaling fish has led to an embodiment of NPF called "particle swarm optimization" (PSO). This theoretical model could be applicable to the behavior of osteoblasts, osteoclasts, and osteocytes, seeing them operating "socially" in response simultaneously to both global and local signals (endocrine, cytokine, mechanical), resulting in their clustered activity at formation and resorption sites. This represents problem-solving by social intelligence, and could potentially add further realism to in silico computer simulation of bone modeling. What insights has NPF provided to bone biology? One example concerns the genetic disorder juvenile Pagets disease or idiopathic hyperphosphatasia, where the anomalous parallel trabecular architecture characteristic of this pathology is consistent with an NPF paradigm by analogy with known experimental NPF systems. Here, coupling or "feedback" between osteoblasts and osteoclasts is the critical element. This NPF paradigm implies a profound link between bone regulation and its architecture: in bone the architecture is the regulation. The former is the emergent

Drawing Architecture Theory on the City

NARCIS (Netherlands)

Altürk, E.

2009-01-01

This study is about architectural drawings. I especially address how drawings operate in architecture theory, and stress a particular role that they play in facilitating critique and speculation as theoretical activities. In this sense, I dwell on the capacity of architectural drawing not only as

The column architecture -- A novel architecture for event driven 2D pixel imagers

International Nuclear Information System (INIS)

Millaud, J.; Nygren, D.

1996-01-01

The authors describe an electronic architecture for two-dimensional pixel arrays that permits very large increases in rate capability for event- or data-driven applications relative to conventional x-y architectures. The column architecture also permits more efficient use of silicon area in applications requiring local buffering, frameless data acquisition, and it avoids entirely the problem of ambiguities that may arise in conventional approaches. Two examples of active implementation are described: high energy physics and protein crystallography

From green architecture to architectural green

DEFF Research Database (Denmark)

Earon, Ofri

2011-01-01

that describes the architectural exclusivity of this particular architecture genre. The adjective green expresses architectural qualities differentiating green architecture from none-green architecture. Currently, adding trees and vegetation to the building’s facade is the main architectural characteristics...... they have overshadowed the architectural potential of green architecture. The paper questions how a green space should perform, look like and function. Two examples are chosen to demonstrate thorough integrations between green and space. The examples are public buildings categorized as pavilions. One......The paper investigates the topic of green architecture from an architectural point of view and not an energy point of view. The purpose of the paper is to establish a debate about the architectural language and spatial characteristics of green architecture. In this light, green becomes an adjective...

A Dual Launch Robotic and Human Lunar Mission Architecture

Science.gov (United States)

Jones, David L.; Mulqueen, Jack; Percy, Tom; Griffin, Brand; Smitherman, David

2010-01-01

This paper describes a comprehensive lunar exploration architecture developed by Marshall Space Flight Center's Advanced Concepts Office that features a science-based surface exploration strategy and a transportation architecture that uses two launches of a heavy lift launch vehicle to deliver human and robotic mission systems to the moon. The principal advantage of the dual launch lunar mission strategy is the reduced cost and risk resulting from the development of just one launch vehicle system. The dual launch lunar mission architecture may also enhance opportunities for commercial and international partnerships by using expendable launch vehicle services for robotic missions or development of surface exploration elements. Furthermore, this architecture is particularly suited to the integration of robotic and human exploration to maximize science return. For surface operations, an innovative dual-mode rover is presented that is capable of performing robotic science exploration as well as transporting human crew conducting surface exploration. The dual-mode rover can be deployed to the lunar surface to perform precursor science activities, collect samples, scout potential crew landing sites, and meet the crew at a designated landing site. With this approach, the crew is able to evaluate the robotically collected samples to select the best samples for return to Earth to maximize the scientific value. The rovers can continue robotic exploration after the crew leaves the lunar surface. The transportation system for the dual launch mission architecture uses a lunar-orbit-rendezvous strategy. Two heavy lift launch vehicles depart from Earth within a six hour period to transport the lunar lander and crew elements separately to lunar orbit. In lunar orbit, the crew transfer vehicle docks with the lander and the crew boards the lander for descent to the surface. After the surface mission, the crew returns to the orbiting transfer vehicle for the return to the Earth. This

Peer-to-peer Cooperative Scheduling Architecture for National Grid Infrastructure

Science.gov (United States)

Matyska, Ludek; Ruda, Miroslav; Toth, Simon

For some ten years, the Czech National Grid Infrastructure MetaCentrum uses a single central PBSPro installation to schedule jobs across the country. This centralized approach keeps a full track about all the clusters, providing support for jobs spanning several sites, implementation for the fair-share policy and better overall control of the grid environment. Despite a steady progress in the increased stability and resilience to intermittent very short network failures, growing number of sites and processors makes this architecture, with a single point of failure and scalability limits, obsolete. As a result, a new scheduling architecture is proposed, which relies on higher autonomy of clusters. It is based on a peer to peer network of semi-independent schedulers for each site or even cluster. Each scheduler accepts jobs for the whole infrastructure, cooperating with other schedulers on implementation of global policies like central job accounting, fair-share, or submission of jobs across several sites. The scheduling system is integrated with the Magrathea system to support scheduling of virtual clusters, including the setup of their internal network, again eventually spanning several sites. On the other hand, each scheduler is local to one of several clusters and is able to directly control and submit jobs to them even if the connection of other scheduling peers is lost. In parallel to the change of the overall architecture, the scheduling system itself is being replaced. Instead of PBSPro, chosen originally for its declared support of large scale distributed environment, the new scheduling architecture is based on the open-source Torque system. The implementation and support for the most desired properties in PBSPro and Torque are discussed and the necessary modifications to Torque to support the MetaCentrum scheduling architecture are presented, too.

Information architecture. Volume 1, The foundations

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-03-01

The Information Management Planning and Architecture Coordinating Team was formed to establish an information architecture framework to meet DOE`s current and future information needs. This department- wide activity was initiated in accordance with the DOE Information Management Strategic Plan; it also supports the Departmental Strategic Plan. It recognizes recent changes in emphasis as reflected in OMB Circular A-130 and the Information Resources Management Planning Process Improvement Team recommendations. Sections of this document provides the foundation for establishing DOE`s Information Architecture: Background, Business Case (reduced duplication of effort, increased integration of activities, improved operational capabilities), Baseline (technology baseline currently in place within DOE), Vision (guiding principles for future DOE Information Architecture), Standards Process, Policy and Process Integration (describes relations between information architecture and business processes), and Next Steps. Following each section is a scenario. A glossary of terms is provided.

Metal active site elasticity linked to activation of homocysteine in methionine synthases

Energy Technology Data Exchange (ETDEWEB)

Koutmos, Markos; Pejchal, Robert; Bomer, Theresa M.; Matthews, Rowena G.; Smith, Janet L.; Ludwig, Martha L. (Michigan)

2008-04-02

Enzymes possessing catalytic zinc centers perform a variety of fundamental processes in nature, including methyl transfer to thiols. Cobalamin-independent (MetE) and cobalamin-dependent (MetH) methionine synthases are two such enzyme families. Although they perform the same net reaction, transfer of a methyl group from methyltetrahydrofolate to homocysteine (Hcy) to form methionine, they display markedly different catalytic strategies, modular organization, and active site zinc centers. Here we report crystal structures of zinc-replete MetE and MetH, both in the presence and absence of Hcy. Structural investigation of the catalytic zinc sites of these two methyltransferases reveals an unexpected inversion of zinc geometry upon binding of Hcy and displacement of an endogenous ligand in both enzymes. In both cases a significant movement of the zinc relative to the protein scaffold accompanies inversion. These structures provide new information on the activation of thiols by zinc-containing enzymes and have led us to propose a paradigm for the mechanism of action of the catalytic zinc sites in these and related methyltransferases. Specifically, zinc is mobile in the active sites of MetE and MetH, and its dynamic nature helps facilitate the active site conformational changes necessary for thiol activation and methyl transfer.

Architecture on Architecture

DEFF Research Database (Denmark)

Olesen, Karen

2016-01-01

that is not scientific or academic but is more like a latent body of data that we find embedded in existing works of architecture. This information, it is argued, is not limited by the historical context of the work. It can be thought of as a virtual capacity – a reservoir of spatial configurations that can...... correlation between the study of existing architectures and the training of competences to design for present-day realities.......This paper will discuss the challenges faced by architectural education today. It takes as its starting point the double commitment of any school of architecture: on the one hand the task of preserving the particular knowledge that belongs to the discipline of architecture, and on the other hand...

Methodological Aspects of Architectural Documentation

Directory of Open Access Journals (Sweden)

Arivaldo Amorim

2011-12-01

Full Text Available This paper discusses the methodological approach that is being developed in the state of Bahia in Brazil since 2003, in architectural and urban sites documentation, using extensive digital technologies. Bahia has a vast territory with important architectural ensembles ranging from the sixteenth century to present day. As part of this heritage is constructed of raw earth and wood, it is very sensitive to various deleterious agents. It is therefore critical document this collection that is under threats. To conduct those activities diverse digital technologies that could be used in documentation process are being experimented. The task is being developed as an academic research, with few financial resources, by scholarship students and some volunteers. Several technologies are tested ranging from the simplest to the more sophisticated ones, used in the main stages of the documentation project, as follows: work overall planning, data acquisition, processing and management and ultimately, to control and evaluate the work. The activities that motivated this paper are being conducted in the cities of Rio de Contas and Lençóis in the Chapada Diamantina, located at 420 km and 750 km from Salvador respectively, in Cachoeira city at Recôncavo Baiano area, 120 km from Salvador, the capital of Bahia state, and at Pelourinho neighbourhood, located in the historic capital. Part of the material produced can be consulted in the website: < www.lcad.ufba.br>.

Quantum mechanical design of enzyme active sites.

Science.gov (United States)

Zhang, Xiyun; DeChancie, Jason; Gunaydin, Hakan; Chowdry, Arnab B; Clemente, Fernando R; Smith, Adam J T; Handel, T M; Houk, K N

2008-02-01

The design of active sites has been carried out using quantum mechanical calculations to predict the rate-determining transition state of a desired reaction in presence of the optimal arrangement of catalytic functional groups (theozyme). Eleven versatile reaction targets were chosen, including hydrolysis, dehydration, isomerization, aldol, and Diels-Alder reactions. For each of the targets, the predicted mechanism and the rate-determining transition state (TS) of the uncatalyzed reaction in water is presented. For the rate-determining TS, a catalytic site was designed using naturalistic catalytic units followed by an estimation of the rate acceleration provided by a reoptimization of the catalytic site. Finally, the geometries of the sites were compared to the X-ray structures of related natural enzymes. Recent advances in computational algorithms and power, coupled with successes in computational protein design, have provided a powerful context for undertaking such an endeavor. We propose that theozymes are excellent candidates to serve as the active site models for design processes.

Information Architecture of Web-Based Interventions to Improve Health Outcomes: Systematic Review.

Science.gov (United States)

Pugatch, Jillian; Grenen, Emily; Surla, Stacy; Schwarz, Mary; Cole-Lewis, Heather

2018-03-21

The rise in usage of and access to new technologies in recent years has led to a growth in digital health behavior change interventions. As the shift to digital platforms continues to grow, it is increasingly important to consider how the field of information architecture (IA) can inform the development of digital health interventions. IA is the way in which digital content is organized and displayed, which strongly impacts users' ability to find and use content. While many information architecture best practices exist, there is a lack of empirical evidence on the role it plays in influencing behavior change and health outcomes. Our aim was to conduct a systematic review synthesizing the existing literature on website information architecture and its effect on health outcomes, behavioral outcomes, and website engagement. To identify all existing information architecture and health behavior literature, we searched articles published in English in the following databases (no date restrictions imposed): ACM Digital Library, CINAHL, Cochrane Library, Google Scholar, Ebsco, and PubMed. The search terms used included information terms (eg, information architecture, interaction design, persuasive design), behavior terms (eg, health behavior, behavioral intervention, ehealth), and health terms (eg, smoking, physical activity, diabetes). The search results were reviewed to determine if they met the inclusion and exclusion criteria created to identify empirical research that studied the effect of IA on health outcomes, behavioral outcomes, or website engagement. Articles that met inclusion criteria were assessed for study quality. Then, data from the articles were extracted using a priori categories established by 3 reviewers. However, the limited health outcome data gathered from the studies precluded a meta-analysis. The initial literature search yielded 685 results, which was narrowed down to three publications that examined the effect of information architecture on

Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox.

Directory of Open Access Journals (Sweden)

Julien Hiblot

Full Text Available Enzymes are proficient catalysts that enable fast rates of Michaelis-complex formation, the chemical step and products release. These different steps may require different conformational states of the active site that have distinct binding properties. Moreover, the conformational flexibility of the active site mediates alternative, promiscuous functions. Here we focused on the lactonase SsoPox from Sulfolobus solfataricus. SsoPox is a native lactonase endowed with promiscuous phosphotriesterase activity. We identified a position in the active site loop (W263 that governs its flexibility, and thereby affects the substrate specificity of the enzyme. We isolated two different sets of substitutions at position 263 that induce two distinct conformational sampling of the active loop and characterized the structural and kinetic effects of these substitutions. These sets of mutations selectively and distinctly mediate the improvement of the promiscuous phosphotriesterase and oxo-lactonase activities of SsoPox by increasing active-site loop flexibility. These observations corroborate the idea that conformational diversity governs enzymatic promiscuity and is a key feature of protein evolvability.

Materials Driven Architectural Design and Representation

DEFF Research Database (Denmark)

Kruse Aagaard, Anders

2015-01-01

This paper aims to outline a framework for a deeper connection between experimentally obtained material knowledge and architectural design. While materials and architecture in the process of realisation are tightly connected, architectural design and representation are often distanced from...... another role in relation to architectural production. It is, in this paper, the intention to point at material research as an active initiator in explorative approaches to architectural design methods and architectural representation. This paper will point at the inclusion of tangible and experimental...... material research in the early phases of architectural design and to that of the architectural set of tools and representation. The paper will through use of existing research and the author’s own material research and practice suggest a way of using a combination of digital drawing, digital fabrication...

Comparison of NMR and crystal structures highlights conformational isomerism in protein active sites

International Nuclear Information System (INIS)

Serrano, Pedro; Pedrini, Bill; Geralt, Michael; Jaudzems, Kristaps; Mohanty, Biswaranjan; Horst, Reto; Herrmann, Torsten; Elsliger, Marc-André; Wilson, Ian A.; Wüthrich, Kurt

2010-01-01

Tools for systematic comparisons of NMR and crystal structures developed by the JCSG were applied to two proteins with known functions: the T. maritima anti-σ factor antagonist TM1081 and the mouse γ-glutamylamine cyclotransferase A2LD1 (gi:13879369). In an attempt to exploit the complementarity of crystal and NMR data, the combined use of the two structure-determination techniques was explored for the initial steps in the challenge of searching proteins of unknown functions for putative active sites. The JCSG has recently developed a protocol for systematic comparisons of high-quality crystal and NMR structures of proteins. In this paper, the extent to which this approach can provide function-related information on the two functionally annotated proteins TM1081, a Thermotoga maritima anti-σ factor antagonist, and A2LD1 (gi:13879369), a mouse γ-glutamylamine cyclotransferase, is explored. The NMR structures of the two proteins have been determined in solution at 313 and 298 K, respectively, using the current JCSG protocol based on the software package UNIO for extensive automation. The corresponding crystal structures were solved by the JCSG at 100 K and 1.6 Å resolution and at 100 K and 1.9 Å resolution, respectively. The NMR and crystal structures of the two proteins share the same overall molecular architectures. However, the precision of the structure determination along the amino-acid sequence varies over a significantly wider range in the NMR structures than in the crystal structures. Thereby, in each of the two NMR structures about 65% of the residues have displacements below the average and in both proteins the less well ordered residues include large parts of the active sites, in addition to some highly solvent-exposed surface areas. Whereas the latter show increased disorder in the crystal and in solution, the active-site regions display increased displacements only in the NMR structures, where they undergo local conformational exchange on the

Development of the nuclear weapons complex EP architecture

Energy Technology Data Exchange (ETDEWEB)

Murray, C.; Halbleib, L.

1996-07-01

The Nuclear Weapons Guidance Team is an interagency committee led by Earl Whiteman, DOE that chartered the generation of EP40100, Concurrent Qualification and its successor EP401099, Concurrent Engineering and Qualification. As this new philosophy of concurrent operations has evolved and as implementation has been initiated, conflicts and insufficiencies in the remaining Engineering Procedures (EPs) have become more apparent. At the Guidance Team meeting in November 1995, this issue was explored and several approaches were considered. It was concluded at this meeting, that a smaller set of interagency EPs described in a hierarchical system could provide the necessary interagency direction to support complex-wide implementation. This set consolidates many existing EP processes where consistency and commonality are critical to success of the extended enterprise. The Guidance Team subsequently chartered an interagency team to initiate development activity associated with the envisioned new EP set. This team had participation from seven Nuclear Weapons Complex (NWC) sites as well as DOE/AL and DP-14 (team members are acknowledged later in this report). Per the Guidance Team, this team, referred to as the Architecture Subcommittee, was to map out and define an EP Architecture for the interagency EPs, make recommendations regarding a more agile process for EP approval and suggest an aggressive timeline to develop the combined EPs. The Architecture Subcommittee was asked to brief their output at the February Guidance Team meeting. This SAND report documents the results of the Architecture Subcommittee`s recommendations.

The value of ancient architecture for educational program of masters of architectural space design

Directory of Open Access Journals (Sweden)

Prishchepa Aleksandr

2017-01-01

Full Text Available The existence of archaeological sites of ancient Greek colony-towns and medieval fortresses gives a real insight into the interaction of all spheres of human activity in ancient times. Ancient Greek Emporium is a vivid example of the architecture, art, archaeology and urban planning synthesis. Archaeological excavations provide an opportunity to study the artefacts of the ancient world belonging to several fields, such as sculpture, decorative arts, fashion design and household. Studying history of archaeology right on the place of excavation of an ancient city masters can imagine the scale of buildings, streets layout and location of business, administrative and residential buildings. It allows students to form professional way of thinking in a short period in order to gather the material and work on the master thesis.

The bacterial response regulator ArcA uses a diverse binding site architecture to regulate carbon oxidation globally.

Directory of Open Access Journals (Sweden)

Dan M Park

Full Text Available Despite the importance of maintaining redox homeostasis for cellular viability, how cells control redox balance globally is poorly understood. Here we provide new mechanistic insight into how the balance between reduced and oxidized electron carriers is regulated at the level of gene expression by mapping the regulon of the response regulator ArcA from Escherichia coli, which responds to the quinone/quinol redox couple via its membrane-bound sensor kinase, ArcB. Our genome-wide analysis reveals that ArcA reprograms metabolism under anaerobic conditions such that carbon oxidation pathways that recycle redox carriers via respiration are transcriptionally repressed by ArcA. We propose that this strategy favors use of catabolic pathways that recycle redox carriers via fermentation akin to lactate production in mammalian cells. Unexpectedly, bioinformatic analysis of the sequences bound by ArcA in ChIP-seq revealed that most ArcA binding sites contain additional direct repeat elements beyond the two required for binding an ArcA dimer. DNase I footprinting assays suggest that non-canonical arrangements of cis-regulatory modules dictate both the length and concentration-sensitive occupancy of DNA sites. We propose that this plasticity in ArcA binding site architecture provides both an efficient means of encoding binding sites for ArcA, σ(70-RNAP and perhaps other transcription factors within the same narrow sequence space and an effective mechanism for global control of carbon metabolism to maintain redox homeostasis.

A Layered Active Memory Architecture for Cognitive Vision Systems

OpenAIRE

Kolonias, Ilias; Christmas, William; Kittler, Josef

2007-01-01

Recognising actions and objects from video material has attracted growing research attention and given rise to important applications. However, injecting cognitive capabilities into computer vision systems requires an architecture more elaborate than the traditional signal processing paradigm for information processing. Inspired by biological cognitive systems, we present a memory architecture enabling cognitive processes (such as selecting the processes required for scene understanding, laye...

The atypical mitogen-activated protein kinase ERK3 is essential for establishment of epithelial architecture.

Science.gov (United States)

Takahashi, Chika; Miyatake, Koichi; Kusakabe, Morioh; Nishida, Eisuke

2018-06-01

Epithelia contribute to physical barriers that protect internal tissues from the external environment and also support organ structure. Accordingly, establishment and maintenance of epithelial architecture are essential for both embryonic development and adult physiology. Here, using gene knockout and knockdown techniques along with gene profiling, we show that extracellular signal-regulated kinase 3 (ERK3), a poorly characterized atypical mitogen-activated protein kinase (MAPK), regulates the epithelial architecture in vertebrates. We found that in Xenopus embryonic epidermal epithelia, ERK3 knockdown impairs adherens and tight-junction protein distribution, as well as tight-junction barrier function, resulting in epidermal breakdown. Moreover, in human epithelial breast cancer cells, inhibition of ERK3 expression induced thickened epithelia with aberrant adherens and tight junctions. Results from microarray analyses suggested that transcription factor AP-2α (TFAP2A), a transcriptional regulator important for epithelial gene expression, is involved in ERK3-dependent changes in gene expression. Of note, TFAP2A knockdown phenocopied ERK3 knockdown in both Xenopus embryos and human cells, and ERK3 was required for full activation of TFAP2A-dependent transcription. Our findings reveal that ERK3 regulates epithelial architecture, possibly together with TFAP2A. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

Architectural Heritage Visualization Using Interactive Technologies

Science.gov (United States)

Albourae, A. T.; Armenakis, C.; Kyan, M.

2017-08-01

With the increased exposure to tourists, historical monuments are at an ever-growing risk of disappearing. Building Information Modelling (BIM) offers a process of digitally documenting of all the features that are made or incorporated into the building over its life-span, thus affords unique opportunities for information preservation. BIM of historical buildings are called Historical Building Information Models (HBIM). This involves documenting a building in detail throughout its history. Geomatics professionals have the potential to play a major role in this area as they are often the first professionals involved on construction development sites for many Architectural, Engineering, and Construction (AEC) projects. In this work, we discuss how to establish an architectural database of a heritage site, digitally reconstruct, preserve and then interact with it through an immersive environment that leverages BIM for exploring historic buildings. The reconstructed heritage site under investigation was constructed in the early 15th century. In our proposed approach, the site selection was based on many factors such as architectural value, size, and accessibility. The 3D model is extracted from the original collected and integrated data (Image-based, range-based, CAD modelling, and land survey methods), after which the elements of the 3D objects are identified by creating a database using the BIM software platform (Autodesk Revit). The use of modern and widely accessible game engine technology (Unity3D) is explored, allowing the user to fully embed and interact with the scene using handheld devices. The details of implementing an integrated pipeline between HBIM, GIS and augmented and virtual reality (AVR) tools and the findings of the work are presented.

ARCHITECTURAL HERITAGE VISUALIZATION USING INTERACTIVE TECHNOLOGIES

Directory of Open Access Journals (Sweden)

A. T. Albourae

2017-08-01

Full Text Available With the increased exposure to tourists, historical monuments are at an ever-growing risk of disappearing. Building Information Modelling (BIM offers a process of digitally documenting of all the features that are made or incorporated into the building over its life-span, thus affords unique opportunities for information preservation. BIM of historical buildings are called Historical Building Information Models (HBIM. This involves documenting a building in detail throughout its history. Geomatics professionals have the potential to play a major role in this area as they are often the first professionals involved on construction development sites for many Architectural, Engineering, and Construction (AEC projects. In this work, we discuss how to establish an architectural database of a heritage site, digitally reconstruct, preserve and then interact with it through an immersive environment that leverages BIM for exploring historic buildings. The reconstructed heritage site under investigation was constructed in the early 15th century. In our proposed approach, the site selection was based on many factors such as architectural value, size, and accessibility. The 3D model is extracted from the original collected and integrated data (Image-based, range-based, CAD modelling, and land survey methods, after which the elements of the 3D objects are identified by creating a database using the BIM software platform (Autodesk Revit. The use of modern and widely accessible game engine technology (Unity3D is explored, allowing the user to fully embed and interact with the scene using handheld devices. The details of implementing an integrated pipeline between HBIM, GIS and augmented and virtual reality (AVR tools and the findings of the work are presented.

Approaching Technical Issues in Architectural Education

DEFF Research Database (Denmark)

Pugnale, Alberto; Parigi, Dario

2012-01-01

This paper discusses teaching of technical subjects in architecture, presenting two experimental activities, recently organized at Aalborg University - a two week long workshop and a one day long lecture. From the pedagogical point of view, the activities are strategically placed between conventi......This paper discusses teaching of technical subjects in architecture, presenting two experimental activities, recently organized at Aalborg University - a two week long workshop and a one day long lecture. From the pedagogical point of view, the activities are strategically placed between...

The architecture of mammalian ribosomal protein promoters

Directory of Open Access Journals (Sweden)

Perry Robert P

2005-02-01

Full Text Available Abstract Background Mammalian ribosomes contain 79 different proteins encoded by widely scattered single copy genes. Coordinate expression of these genes at transcriptional and post-transcriptional levels is required to ensure a roughly equimolar accumulation of ribosomal proteins. To date, detailed studies of only a very few ribosomal protein (rp promoters have been made. To elucidate the general features of rp promoter architecture, I made a detailed sequence comparison of the promoter regions of the entire set of orthologous human and mouse rp genes. Results A striking evolutionarily conserved feature of most rp genes is the separation by an intron of the sequences involved in transcriptional and translational regulation from the sequences with protein encoding function. Another conserved feature is the polypyrimidine initiator, which conforms to the consensus (Y2C+1TY(T2(Y3. At least 60 % of the rp promoters contain a largely conserved TATA box or A/T-rich motif, which should theoretically have TBP-binding capability. A remarkably high proportion of the promoters contain conserved binding sites for transcription factors that were previously implicated in rp gene expression, namely upstream GABP and Sp1 sites and downstream YY1 sites. Over 80 % of human and mouse rp genes contain a transposable element residue within 900 bp of 5' flanking sequence; very little sequence identity between human and mouse orthologues was evident more than 200 bp upstream of the transcriptional start point. Conclusions This analysis has provided some valuable insights into the general architecture of mammalian rp promoters and has identified parameters that might coordinately regulate the transcriptional activity of certain subsets of rp genes.

Architecture and aesthetics of nuclear power plants

International Nuclear Information System (INIS)

Andreu, P.

1977-01-01

Having first analysed the main aesthetical and architectural problems related to the establishment of nuclear sites, the first results of the description is given of studies undertaken by a group of architects asked by E.D.F. to conceive the main buildings of a nuclear power plant and to imagine their insert in the site [fr

Calcium-manganese oxides as structural and functional models for active site in oxygen evolving complex in photosystem II: lessons from simple models.

Science.gov (United States)

Najafpour, Mohammad Mahdi

2011-01-01

The oxygen evolving complex in photosystem II which induces the oxidation of water to dioxygen in plants, algae and certain bacteria contains a cluster of one calcium and four manganese ions. It serves as a model to split water by sunlight. Reports on the mechanism and structure of photosystem II provide a more detailed architecture of the oxygen evolving complex and the surrounding amino acids. One challenge in this field is the development of artificial model compounds to study oxygen evolution reaction outside the complicated environment of the enzyme. Calcium-manganese oxides as structural and functional models for the active site of photosystem II are explained and reviewed in this paper. Because of related structures of these calcium-manganese oxides and the catalytic centers of active site of the oxygen evolving complex of photosystem II, the study may help to understand more about mechanism of oxygen evolution by the oxygen evolving complex of photosystem II. Copyright © 2010 Elsevier B.V. All rights reserved.

Future space. A new blueprint for business architecture.

Science.gov (United States)

Huang, J

2001-04-01

Although the Internet is an essential conduit for many business activities, it isn't rendering the physical world any less important, as the failures of many Web merchants demonstrate. People need social and sensual contact. The companies that succeed will be those best able to integrate the physical and the virtual. But that requires a new kind of business architecture--a new approach to designing stores, offices, factories, and other spaces where business is conducted. The author, a faculty member at Harvard Graduate School of Design, provides practical guidelines to help managers and entrepreneurs think creatively about the structures in which their businesses operate. He outlines four challenges facing designers of such "convergent" structures, so-called because they function in both physical and virtual space: matching form to function, allowing visitors to visualize the presence of others, personalizing spaces, and choreographing connectivity. Using numerous examples, from a fashion retailer that wants to sell in stores as well as through a Web site to a radically new kind of consulate, the author shows how businesses can meet each challenge. For instance, allowing customers to visualize the presence of others means that visitors to a Web site should be given a sense of other site visitors. Personalizing physical and virtual spaces involves using databases to enable those spaces to adapt quickly to user preferences. The success of companies attempting to merge on-line and traditional operations will depend on many factors. But without a well-designed convergent architecture, no company will fully reap the synergies of physical space and Internet technology.

ERECTA signaling controls Arabidopsis inflorescence architecture through chromatin-mediated activation of PRE1 expression.

Science.gov (United States)

Cai, Hanyang; Zhao, Lihua; Wang, Lulu; Zhang, Man; Su, Zhenxia; Cheng, Yan; Zhao, Heming; Qin, Yuan

2017-06-01

Flowering plants display a remarkable diversity in inflorescence architecture, and pedicel length is one of the key contributors to this diversity. In Arabidopsis thaliana, the receptor-like kinase ERECTA (ER) mediated signaling pathway plays important roles in regulating inflorescence architecture by promoting cell proliferation. However, the regulating mechanism remains elusive in the pedicel. Genetic interactions between ERECTA signaling and the chromatin remodeling complex SWR1 in the control of inflorescence architecture were studied. Comparative transcriptome analysis was applied to identify downstream components. Chromatin immunoprecipitation and nucleosome occupancy was further investigated. The results indicated that the chromatin remodeler SWR1 coordinates with ERECTA signaling in regulating inflorescence architecture by activating the expression of PRE1 family genes and promoting pedicel elongation. It was found that SWR1 is required for the incorporation of the H2A.Z histone variant into nucleosomes of the whole PRE1 gene family and the ERECTA controlled expression of PRE1 gene family through regulating nucleosome dynamics. We propose that utilization of a chromatin remodeling complex to regulate gene expression is a common theme in developmental control across kingdoms. These findings shed light on the mechanisms through which chromatin remodelers orchestrate complex transcriptional regulation of gene expression in coordination with a developmental cue. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Half-of-the-sites reactivity of outer-membrane phospholipase A against an active-site-directed inhibitor.

Science.gov (United States)

Ubarretxena-Belandia, I; Cox, R C; Dijkman, R; Egmond, M R; Verheij, H M; Dekker, N

1999-03-01

The reaction of a novel active-site-directed phospholipase A1 inhibitor with the outer-membrane phospholipase A (OMPLA) was investigated. The inhibitor 1-p-nitrophenyl-octylphosphonate-2-tridecylcarbamoyl-3-et hanesulfonyl -amino-3-deoxy-sn-glycerol irreversibly inactivated OMPLA. The inhibition reaction did not require the cofactor calcium or an unprotonated active-site His142. The inhibition of the enzyme solubilized in hexadecylphosphocholine micelles was characterized by a rapid (t1/2 = 20 min) and complete loss of enzymatic activity, concurrent with the covalent modification of 50% of the active-site serines, as judged from the amount of p-nitrophenolate (PNP) released. Modification of the remaining 50% occurred at a much lower rate, indicative of half-of-the-sites reactivity against the inhibitor of this dimeric enzyme. Inhibition of monomeric OMPLA solubilized in hexadecyl-N,N-dimethyl-1-ammonio-3-propanesulfonate resulted in an equimolar monophasic release of PNP, concurrent with the loss of enzymatic activity (t1/2 = 14 min). The half-of-the-sites reactivity is discussed in view of the dimeric nature of this enzyme.

WHALE, a management tool for Tier-2 LCG sites

Science.gov (United States)

Barone, L. M.; Organtini, G.; Talamo, I. G.

2012-12-01

The LCG (Worldwide LHC Computing Grid) is a grid-based hierarchical computing distributed facility, composed of more than 140 computing centers, organized in 4 tiers, by size and offer of services. Every site, although indipendent for many technical choices, has to provide services with a well-defined set of interfaces. For this reason, different LCG sites need frequently to manage very similar situations, like jobs behaviour on the batch system, dataset transfers between sites, operating system and experiment software installation and configuration, monitoring of services. In this context we created WHALE (WHALE Handles Administration in an LCG Environment), a software actually used at the T2_IT_Rome site, an LCG Tier-2 for the CMS experiment. WHALE is a generic, site independent tool written in Python: it allows administrator to interact in a uniform and coherent way with several subsystems using a high level syntax which hides specific commands. The architecture of WHALE is based on the plugin concept and on the possibility of connecting the output of a plugin to the input of the next one, in a pipe-like system, giving the administrator the possibility of making complex functions by combining the simpler ones. The core of WHALE just handles the plugin orchestrations, while even the basic functions (eg. the WHALE activity logging) are performed by plugins, giving the capability to tune and possibly modify every component of the system. WHALE already provides many plugins useful for a LCG site and some more for a Tier-2 of the CMS experiment, especially in the field of job management, dataset transfer and analysis of performance results and availability tests (eg. Nagios tests, SAM tests). Thanks to its architecture and the provided plugins WHALE makes easy to perform tasks that, even if logically simple, are technically complex or tedious, like eg. closing all the worker nodes with a job-failure rate greater than a given threshold. Finally, thanks to the

Monumentality vs. everyday life: architecture's public role

Directory of Open Access Journals (Sweden)

Joaquim Manoel Guedes Sobrinho

2007-06-01

Full Text Available Conference held in Belo Horizonte, Minas Gerais, on March 18, 2006. Thoughts about architecture and the nation." Architecture is everything dealing with construction." (ARGAN, c. 1992. An object's cultural and ethical value is measured by how well it addresses demand, which in turn is related to the planet Earth, to scarce resources, and to people's survival. Architecture, its craft, and its teaching are studied from the perspective of the act, which is the art of building, to meet social, public and private demands through historical processes of power games, at a given place and time. Architecture is reduced to its essence to unveil the roots of its emergence and understand the architect's responsibilities in the new mass society, when we enter this" new popular period in history" (SANTOS; SOUZA, c1998. We suggest the following sequence of procedures as a path of knowledge and to support the free development of invention: 1 Initially assess the prospective problem to choose and study the location and the tasks of the architect, the client, society, and government; 2 identify and understand building plans for that location; 3 study the activities, flows, quantity, quality and combination of spaces resulting from the conflicting yearnings of people, in line with social needs and translated into private and public building plans; 4 graphically analyze the sectorial subsystems of areas associated according to affinities, considering their arrangement and organization in the site; 5 work on the rigorous construction of architecture, which in final analysis is matter and form; It should be pointed out that it is necessary to: 6 expand the supply of information on specific architectural problems to be addressed, and to organize the actual and effective involvement of people in the discussion of projects and works of their interest, unlike assembly and participative meetings; 7 expand opportunities to allow a greater number of architects to take part

Salt site performance assessment activities

International Nuclear Information System (INIS)

Kircher, J.F.; Gupta, S.K.

1983-01-01

During this year the first selection of the tools (codes) for performance assessments of potential salt sites have been tentatively selected and documented; the emphasis has shifted from code development to applications. During this period prior to detailed characterization of a salt site, the focus is on bounding calculations, sensitivity and with the data available. The development and application of improved methods for sensitivity and uncertainty analysis is a focus for the coming years activities and the subject of a following paper in these proceedings. Although the assessments to date are preliminary and based on admittedly scant data, the results indicate that suitable salt sites can be identified and repository subsystems designed which will meet the established criteria for protecting the health and safety of the public. 36 references, 5 figures, 2 tables

Archaeal promoter architecture and mechanism of gene activation

DEFF Research Database (Denmark)

Peng, Nan; Ao, Xiang; Liang, Yun Xiang

2011-01-01

element named ara box directing arabinose-inducible expression and the basal promoter element TATA, serving as the binding site for the TATA-binding protein. Strikingly, these promoters possess a modular structure that allows an essentially inactive basal promoter to be strongly activated. The invoked...... mechanisms include TFB (transcription factor B) recruitment by the ara-box-binding factor to activate gene expression and modulation of TFB recruitment efficiency to yield differential gene expression....

Activate: Social Action in landscape Architecture Design Education

Directory of Open Access Journals (Sweden)

Helen Mclean

2004-06-01

Full Text Available This paper is a reflection on the teaching approach adopted in two landscape architecture design studios where students were engaged in developing design projects and campaign strategies informed by personal understandings of action and advocacy for and with communities. The approach was a deliberate application of principles from advocacy theory enmeshed in an educational philosophy of learner-centeredness and the intent was for students to develop strong individual senses of awareness and empowerment in an environment that value personal interests and learning. An evaluation of the approach as an attempt to position landscape architecture as social practice is provided.

Sustainable Education Campus in Spain: Nature and Architecture for Training

Science.gov (United States)

Calvo-Sotelo, Pablo Campos

2008-01-01

The quality of education is intimately linked to its architecture. Any urbanistic/architectural project must stem from an in-depth study of the area's characteristics, taken in the broad geographical, climatic, cultural, functional and ideological sense. The site should provide the conceptual energy from which a campus draws life. This requirement…

Space-Time and Architecture

Science.gov (United States)

Field, F.; Goodbun, J.; Watson, V.

Architects have a role to play in interplanetary space that has barely yet been explored. The architectural community is largely unaware of this new territory, for which there is still no agreed method of practice. There is moreover a general confusion, in scientific and related fields, over what architects might actually do there today. Current extra-planetary designs generally fail to explore the dynamic and relational nature of space-time, and often reduce human habitation to a purely functional problem. This is compounded by a crisis over the representation (drawing) of space-time. The present work returns to first principles of architecture in order to realign them with current socio-economic and technological trends surrounding the space industry. What emerges is simultaneously the basis for an ecological space architecture, and the representational strategies necessary to draw it. We explore this approach through a work of design-based research that describes the construction of Ocean; a huge body of water formed by the collision of two asteroids at the Translunar Lagrange Point (L2), that would serve as a site for colonisation, and as a resource to fuel future missions. Ocean is an experimental model for extra-planetary space design and its representation, within the autonomous discipline of architecture.

Information architecture. Volume 2, Part 1: Baseline analysis summary

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-12-01

The Department of Energy (DOE) Information Architecture, Volume 2, Baseline Analysis, is a collaborative and logical next-step effort in the processes required to produce a Departmentwide information architecture. The baseline analysis serves a diverse audience of program management and technical personnel and provides an organized way to examine the Department`s existing or de facto information architecture. A companion document to Volume 1, The Foundations, it furnishes the rationale for establishing a Departmentwide information architecture. This volume, consisting of the Baseline Analysis Summary (part 1), Baseline Analysis (part 2), and Reference Data (part 3), is of interest to readers who wish to understand how the Department`s current information architecture technologies are employed. The analysis identifies how and where current technologies support business areas, programs, sites, and corporate systems.

Active site - a site of binding of affinity inhibitors in baker's yeast inorganic pyrophosphatase

International Nuclear Information System (INIS)

Svyato, I.E.; Sklyankina, V.A.; Avaeva, S.M.

1986-01-01

The interaction of the enzyme-substrate complex with methyl phosphate, O-phosphoethanolamine, O-phosphopropanolamine, N-acetylphosphoserine, and phosphoglyolic acid, as well as pyrophosphatase, modified by monoesters of phosphoric acid, with pyrophosphate and tripolyphosphate, was investigated. It was shown that the enzyme containing the substrate in the active site does not react with monophosphates, but modified pyrophosphatase entirely retains the ability to bind polyanions to the regulatory site. It is concluded that the inactivation of baker's yeast inorganic pyrophosphatase by monoesters of phosphoric acid, which are affinity inhibitors of it, is the result of modification of the active site of the enzyme

Architecture effects on multivalent interactions by polypeptide-based multivalent ligands

Science.gov (United States)

Liu, Shuang

Multivalent interactions are characterized by the simultaneous binding between multiple ligands and multiple binding sites, either in solutions or at interfaces. In biological systems, most multivalent interactions occur between protein receptors and carbohydrate ligands through hydrogen-bonding and hydrophobic interactions. Compared with weak affinity binding between one ligand and one binding site, i.e. monovalent interaction, multivalent interactioins provide greater avidity and specificity, and therefore play unique roles in a broad range of biological activities. Moreover, the studies of multivalent interactions are also essential for producing effective inhibitors and effectors of biological processes that could have important therapeutic applications. Synthetic multivalent ligands have been designed to mimic the biological functions of natural multivalent interactions, and various types of scaffolds have been used to display multiple ligands, including small molecules, linear polymers, dendrimers, nanoparticle surfaces, monolayer surfaces and liposomes. Studies have shown that multivalent interactions can be highly affected by various architectural parameters of these multivalent ligands, including ligand identities, valencies, spacing, ligand densities, nature of linker arms, scaffold length and scaffold conformation. Most of these multivalent ligands are chemically synthesized and have limitations of controlling over sequence and conformation, which is a barrier for mimicking ordered and controlled natural biological systems. Therefore, multivalent ligands with precisely controlled architecture are required for improved structure-function relationship studies. Protein engineering methods with subsequent chemical coupling of ligands provide significant advantages of controlling over backbone conformation and functional group placement, and therefore have been used to synthesize recombinant protein-based materials with desired properties similar to natural

Managing Siting Activities for Nuclear Power Plants

International Nuclear Information System (INIS)

2012-01-01

One of the IAEA's statutory objectives is to ''seek to accelerate and enlarge the contribution of atomic energy to peace, health and prosperity throughout the world''. One way this objective is achieved is through the publication of a range of technical series. Two of these are the IAEA Nuclear Energy Series and the IAEA Safety Standards Series. According to Article III.A.6 of the IAEA Statute, the safety standards establish 'standards of safety for protection of health and minimization of danger to life and property.' The safety standards include the Safety Fundamentals, Safety Requirements and Safety Guides. These standards are written primarily in a regulatory style, and are binding on the IAEA for its own programmes. The principal users are the regulatory bodies in Member States and other national authorities. The IAEA Nuclear Energy Series comprises reports designed to encourage and assist R and D on, and application of, nuclear energy for peaceful uses. This includes practical examples to be used by owners and operators of utilities in Member States, implementing organizations, academia, and government officials, among others. This information is presented in guides, reports on technology status and advances, and best practices for peaceful uses of nuclear energy based on inputs from international experts. The IAEA Nuclear Energy Series complements the IAEA Safety Standards Series. The introduction of nuclear power brings new challenges to States - one of them being the selection of appropriates sites. It is a project that needs to begin early, be well managed, and deploy good communications with all stakeholders; including regulators. This is important, not just for those States introducing nuclear power for the first time, but for any State looking to build a new nuclear power plant. The purpose of the siting activities goes beyond choosing a suitable site and acquiring a licence. A large part of the project is about producing and maintaining a validated

Managing Siting Activities for Nuclear Power Plants

Energy Technology Data Exchange (ETDEWEB)

NONE

2012-06-15

One of the IAEA's statutory objectives is to ''seek to accelerate and enlarge the contribution of atomic energy to peace, health and prosperity throughout the world''. One way this objective is achieved is through the publication of a range of technical series. Two of these are the IAEA Nuclear Energy Series and the IAEA Safety Standards Series. According to Article III.A.6 of the IAEA Statute, the safety standards establish 'standards of safety for protection of health and minimization of danger to life and property.' The safety standards include the Safety Fundamentals, Safety Requirements and Safety Guides. These standards are written primarily in a regulatory style, and are binding on the IAEA for its own programmes. The principal users are the regulatory bodies in Member States and other national authorities. The IAEA Nuclear Energy Series comprises reports designed to encourage and assist R and D on, and application of, nuclear energy for peaceful uses. This includes practical examples to be used by owners and operators of utilities in Member States, implementing organizations, academia, and government officials, among others. This information is presented in guides, reports on technology status and advances, and best practices for peaceful uses of nuclear energy based on inputs from international experts. The IAEA Nuclear Energy Series complements the IAEA Safety Standards Series. The introduction of nuclear power brings new challenges to States - one of them being the selection of appropriates sites. It is a project that needs to begin early, be well managed, and deploy good communications with all stakeholders; including regulators. This is important, not just for those States introducing nuclear power for the first time, but for any State looking to build a new nuclear power plant. The purpose of the siting activities goes beyond choosing a suitable site and acquiring a licence. A large part of the project is about producing and maintaining a validated

Active House: an all active eco-architecture building envelope concept

NARCIS (Netherlands)

Zeiler, W.

2008-01-01

The present trend in energy efficient eco-architecture dwellings is the passive house concept. The ventilation of many of these passive houses is critical. The development of sustainable buildings is driven by the need to preserve the balance of nature. The ventilation of many of these passive

Direct instrumental identification of catalytically active surface sites

Science.gov (United States)

Pfisterer, Jonas H. K.; Liang, Yunchang; Schneider, Oliver; Bandarenka, Aliaksandr S.

2017-09-01

The activity of heterogeneous catalysts—which are involved in some 80 per cent of processes in the chemical and energy industries—is determined by the electronic structure of specific surface sites that offer optimal binding of reaction intermediates. Directly identifying and monitoring these sites during a reaction should therefore provide insight that might aid the targeted development of heterogeneous catalysts and electrocatalysts (those that participate in electrochemical reactions) for practical applications. The invention of the scanning tunnelling microscope (STM) and the electrochemical STM promised to deliver such imaging capabilities, and both have indeed contributed greatly to our atomistic understanding of heterogeneous catalysis. But although the STM has been used to probe and initiate surface reactions, and has even enabled local measurements of reactivity in some systems, it is not generally thought to be suited to the direct identification of catalytically active surface sites under reaction conditions. Here we demonstrate, however, that common STMs can readily map the catalytic activity of surfaces with high spatial resolution: we show that by monitoring relative changes in the tunnelling current noise, active sites can be distinguished in an almost quantitative fashion according to their ability to catalyse the hydrogen-evolution reaction or the oxygen-reduction reaction. These data allow us to evaluate directly the importance and relative contribution to overall catalyst activity of different defects and sites at the boundaries between two materials. With its ability to deliver such information and its ready applicability to different systems, we anticipate that our method will aid the rational design of heterogeneous catalysts.

Role of active site rigidity in activity: MD simulation and fluorescence study on a lipase mutant.

Directory of Open Access Journals (Sweden)

Md Zahid Kamal

Full Text Available Relationship between stability and activity of enzymes is maintained by underlying conformational flexibility. In thermophilic enzymes, a decrease in flexibility causes low enzyme activity while in less stable proteins such as mesophiles and psychrophiles, an increase in flexibility is associated with enhanced enzyme activity. Recently, we identified a mutant of a lipase whose stability and activity were enhanced simultaneously. In this work, we probed the conformational dynamics of the mutant and the wild type lipase, particularly flexibility of their active site using molecular dynamic simulations and time-resolved fluorescence techniques. In contrast to the earlier observations, our data show that active site of the mutant is more rigid than wild type enzyme. Further investigation suggests that this lipase needs minimal reorganization/flexibility of active site residues during its catalytic cycle. Molecular dynamic simulations suggest that catalytically competent active site geometry of the mutant is relatively more preserved than wild type lipase, which might have led to its higher enzyme activity. Our study implies that widely accepted positive correlation between conformation flexibility and enzyme activity need not be stringent and draws attention to the possibility that high enzyme activity can still be accomplished in a rigid active site and stable protein structures. This finding has a significant implication towards better understanding of involvement of dynamic motions in enzyme catalysis and enzyme engineering through mutations in active site.

Information architecture. Volume 4: Vision

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

The Vision document marks the transition from definition to implementation of the Department of Energy (DOE) Information Architecture Program. A description of the possibilities for the future, supported by actual experience with a process model and tool set, points toward implementation options. The directions for future information technology investments are discussed. Practical examples of how technology answers the business and information needs of the organization through coordinated and meshed data, applications, and technology architectures are related. This document is the fourth and final volume in the planned series for defining and exhibiting the DOE information architecture. The targeted scope of this document includes DOE Program Offices, field sites, contractor-operated facilities, and laboratories. This document paints a picture of how, over the next 7 years, technology may be implemented, dramatically improving the ways business is conducted at DOE. While technology is mentioned throughout this document, the vision is not about technology. The vision concerns the transition afforded by technology and the process steps to be completed to ensure alignment with business needs. This goal can be met if those directing the changing business and mission-support processes understand the capabilities afforded by architectural processes.

Unrealized Architectural Projects in Lithuania: Historical Development

Directory of Open Access Journals (Sweden)

Indrė Gudelytė

2012-05-01

Full Text Available The “unrealized architectural projects” are the building projects, carried out under the specific design task and intended to be built in a particular place (site, though, for certain reasons and circumstances, have never been constructed. However, up to the present day, the topic of the “unbuilt” has been analyzed just episodically in literature and sources. The article touches upon the historical development of unrealized architectural works, as well as their artistic value and role within various historical periods of Lithuanian architecture. One of the chapters briefly reviews the relevance and development of unrealized projects during the period since Czarist Russian occupation (1795 to the restoration of Lithuanian independence (1990. Furthermore, the deeper analysis of the Soviet period (1940–1990 “dead” architecture is presented. While exploring “the unrealized”, attention has been also paid to what was actu ally built, therefore the prevailing architectural styles, tendencies and examples of the corresponding decade (in Lithuania and worldwide have been studied.Article in Lithuanian

Architecture of the Product State Model Environment

DEFF Research Database (Denmark)

Holm Larsen, Michael; Lynggaard, Hans Jørgen B.

2003-01-01

on thedevelopment activities of the PSM architecture. An example discusses how to handle product relatedinformation on the shop floor in a manufacturing company and focuses on how dynamically updatedproduct data can improve control of production activities. This prototype example of welding a jointbetween two steel...... plates serves as proof of concept for the PSM architecture....

Active sites environmental monitoring Program - Program Plan: Revision 2

International Nuclear Information System (INIS)

Morrissey, C.M.; Hicks, D.S.; Ashwood, T.L.; Cunningham, G.R.

1994-05-01

The Active Sites Environmental Monitoring Program (ASEMP), initiated in 1989, provides early detection and performance monitoring of active low-level-waste (LLW) and transuranic (TRU) waste facilities at Oak Ridge National Laboratory (ORNL). Several changes have recently occurred in regard to the sites that are currently used for waste storage and disposal. These changes require a second set of revisions to the ASEMP program plan. This document incorporates those revisions. This program plan presents the organization and procedures for monitoring the active sites. The program plan also provides internal reporting levels to guide the evaluation of monitoring results

Documenting Architectural Heritage in Bahia, Brazil, Using Spherical Photogrammetry

Science.gov (United States)

De Amorim, A. L.; Fangi, G.; Malinverni, E. S.

2013-07-01

The Cultural Heritage disappears at a rate higher than we are able, not only, to restore but also to document: human and natural factors, negligence or worst, deliberate demolitions put in danger the collective Architectural Heritage (AH). According to CIPA statements, the recording is important and has to follow some guidelines. The Architectural and Urban Heritage data have to be historically related, critically assessed and analyzed, before to be organized according to a thematic structure and become available for further uses. This paper shows the experiences developed by the Laboratory of Computer Graphics applied to Architecture and Design (LCAD), at the Architecture School of the Federal University of Bahia (FAUFBA), Brazil, in cooperation with the Università Politecnica delle Marche (UNIVPM, DICEA Department), Italy, in documenting architectural heritage. The research set up now has been carried out in the historical sites of Bahia, as Pelourinho neighborhood, a World Heritage by UNESCO. Other historical sites are in the plan of this survey, like the cities of Lençóis and Mucugê in Chapada Diamantina region. The aim is to build a technological platform based on low cost digital technologies and open source tools, such as Panoramic Spherical Photogrammetry, Spatial Database, Geographic Information Systems, Three-dimensional Geometric Modeling, CAD technology, for the collection, validation and dissemination of AH.

Site-Specific Soundscape Design for the Creation of Sonic Architectures and the Emergent Voices of Buildings

Directory of Open Access Journals (Sweden)

Jordan Lacey

2014-01-01

Full Text Available Does a building contain its own Voice? And if so, can that Voice be discovered, transformed and augmented by soundscape design? Barry Blesser’s writings on acoustic space, discuss reverberation and resonant frequencies as providing architectural spaces with characteristic listening conditions related to the architectural space’s dimensions and materiality. The paper argues that Blesser and Salter expand such discussion into pantheistic speculation when suggesting that humanity contains the imaginative capacity to experience spaces as “living spirits”. This argument is achieved by building on the speculation through the discussion of a soundscape design methodology that considers space as containing pantheistic qualities. Sonic architectures are created with electroacoustic sound installations that recompose existing architectural soundscapes, to create the conditions for the emergence of the Voices of buildings. This paper describes two soundscape designs, Revoicing the Striated Soundscape and Subterranean Voices, which transformed existing architectural soundscapes for the emergence of Voices in a laneway and a building located in the City of Melbourne, Australia.

Camp Site City, suburban porosity and eclecticism in San José, Costa Rica.

NARCIS (Netherlands)

Doevendans, C.H.; Schram, A.L.; Heynen, Hilde; Meulder, Bruno de

2005-01-01

The notion of 'camp' seems opposed to the more solid city and its almost permanent architecture. In this contribution, we regard the camp as a spatial concept with a twofold appearance: as both repressing and freeing, as a site for both larger, planned strategic activities, and smaller scale tactic

Basic design in architectural education in Turkey

Directory of Open Access Journals (Sweden)

Makaklı Elif Süyük

2016-01-01

Full Text Available Creative activity is one of the most significant parts of architectural education. In the architectural curriculum organisation ‘design studio’ is the unique and preeminent subject. Freshman students encounter ‘design’ first in basic design course, before they know what designing incorporates. The Basic Design is the starting point of initiating creativity in architecture education. It helps each student to understand architecture as a creative and innovative practice in the first year of education. This study analyzes the characteristics of basic design course, the topics of the course contents and its significance in the architectural curriculum in Turkey as well.

Reframing information architecture

CERN Document Server

Resmini, Andrea

2014-01-01

Information architecture has changed dramatically since the mid-1990s and earlier conceptions of the world and the internet being different and separate have given way to a much more complex scenario in the present day. In the post-digital world that we now inhabit the digital and the physical blend easily and our activities and usage of information takes place through multiple contexts and via multiple devices and unstable, emergent choreographies. Information architecture now is steadily growing into a channel- or medium-specific multi-disciplinary framework, with contributions coming from a

A resource management architecture for metacomputing systems.

Energy Technology Data Exchange (ETDEWEB)

Czajkowski, K.; Foster, I.; Karonis, N.; Kesselman, C.; Martin, S.; Smith, W.; Tuecke, S.

1999-08-24

Metacomputing systems are intended to support remote and/or concurrent use of geographically distributed computational resources. Resource management in such systems is complicated by five concerns that do not typically arise in other situations: site autonomy and heterogeneous substrates at the resources, and application requirements for policy extensibility, co-allocation, and online control. We describe a resource management architecture that addresses these concerns. This architecture distributes the resource management problem among distinct local manager, resource broker, and resource co-allocator components and defines an extensible resource specification language to exchange information about requirements. We describe how these techniques have been implemented in the context of the Globus metacomputing toolkit and used to implement a variety of different resource management strategies. We report on our experiences applying our techniques in a large testbed, GUSTO, incorporating 15 sites, 330 computers, and 3600 processors.

Protein Kinases in Shaping Plant Architecture.

Science.gov (United States)

Wu, Juan; Wang, Bo; Xin, Xiaoyun; Ren, Dongtao

2018-02-13

Plant architecture, the three-dimensional organization of the plant body, includes the branching pattern and the size, shape, and position of organs. Plant architecture is genetically controlled and is influenced by environmental conditions. The regulations occur at most of the stages from the first division of the fertilized eggs to the final establishment of plant architecture. Among the various endogenous regulators, protein kinases and their associated signaling pathways have been shown to play important roles in regulating the process of plant architecture establishment. In this review, we summarize recent progress in the understanding of the mechanisms by which plant architecture formation is regulated by protein kinases, especially mitogen-activated protein kinase (MAPK). Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

The Experimental Physics and Industrial Control System architecture: Past, present, and future

International Nuclear Information System (INIS)

Dalesio, L.R.; Hill, J.O.; Kraimer, M.; Lewis, S.; Murray, D.; Hunt, S.; Claussen, M.; Watson, W.

1993-01-01

The Experimental Physics and Industrial Control System (EPICS), has been used at a number of sites for performing data acquisition, supervisory control, closed-loop control, sequential control, and operational optimization. The EPICS architecture was originally developed by a group with diverse backgrounds in physics and industrial control. The current architecture represents one instance of the ''standard model.'' It provides distributed processing and communication from any LAN device to the front end controllers. This paper will present the genealogy, current architecture, performance envelope, current installations, and planned extensions for requirements not met by the current architecture

Promoter proximal polyadenylation sites reduce transcription activity

DEFF Research Database (Denmark)

Andersen, Pia Kjølhede; Lykke-Andersen, Søren; Jensen, Torben Heick

2012-01-01

Gene expression relies on the functional communication between mRNA processing and transcription. We previously described the negative impact of a point-mutated splice donor (SD) site on transcription. Here we demonstrate that this mutation activates an upstream cryptic polyadenylation (CpA) site......, which in turn causes reduced transcription. Functional depletion of U1 snRNP in the context of the wild-type SD triggers the same CpA event accompanied by decreased RNA levels. Thus, in accordance with recent findings, U1 snRNP can shield premature pA sites. The negative impact of unshielded pA sites...... on transcription requires promoter proximity, as demonstrated using artificial constructs and supported by a genome-wide data set. Importantly, transcription down-regulation can be recapitulated in a gene context devoid of splice sites by placing a functional bona fide pA site/transcription terminator within ∼500...

Energy transfer at the active sites of heme proteins

International Nuclear Information System (INIS)

Dlott, D.D.; Hill, J.R.

1995-01-01

Experiments using a picosecond pump-probe apparatus at the Picosecond Free-electron Laser Center at Stanford University, were performed to investigate the relaxation of carbon monoxide bound to the active sites of heme proteins. The significance of these experiments is two-fold: (1) they provide detailed information about molecular dynamics occurring at the active sites of proteins; and (2) they provide insight into the nature of vibrational relaxation processes in condensed matter. Molecular engineering is used to construct various molecular systems which are studied with the FEL. We have studied native proteins, mainly myoglobin obtained from different species, mutant proteins produced by genetic engineering using recombinant DNA techniques, and a variety of model systems which mimic the structures of the active sites of native proteins, which are produced using molecular synthesis. Use of these different systems permits us to investigate how specific molecular structural changes affect dynamical processes occurring at the active sites. This research provides insight into the problems of how different species needs are fulfilled by heme proteins which have greatly different functionality, which is induced by rather small structural changes

Dynamics of translocation and substrate binding in individual complexes formed with active site mutants of {phi}29 DNA polymerase.

Science.gov (United States)

Dahl, Joseph M; Wang, Hongyun; Lázaro, José M; Salas, Margarita; Lieberman, Kate R

2014-03-07

The Φ29 DNA polymerase (DNAP) is a processive B-family replicative DNAP. Fluctuations between the pre-translocation and post-translocation states can be quantified from ionic current traces, when individual Φ29 DNAP-DNA complexes are held atop a nanopore in an electric field. Based upon crystal structures of the Φ29 DNAP-DNA binary complex and the Φ29 DNAP-DNA-dNTP ternary complex, residues Tyr-226 and Tyr-390 in the polymerase active site were implicated in the structural basis of translocation. Here, we have examined the dynamics of translocation and substrate binding in complexes formed with the Y226F and Y390F mutants. The Y226F mutation diminished the forward and reverse rates of translocation, increased the affinity for dNTP in the post-translocation state by decreasing the dNTP dissociation rate, and increased the affinity for pyrophosphate in the pre-translocation state. The Y390F mutation significantly decreased the affinity for dNTP in the post-translocation state by decreasing the association rate ∼2-fold and increasing the dissociation rate ∼10-fold, implicating this as a mechanism by which this mutation impedes DNA synthesis. The Y390F dissociation rate increase is suppressed when complexes are examined in the presence of Mn(2+) rather than Mg(2+). The same effects of the Y226F or Y390F mutations were observed in the background of the D12A/D66A mutations, located in the exonuclease active site, ∼30 Å from the polymerase active site. Although translocation rates were unaffected in the D12A/D66A mutant, these exonuclease site mutations caused a decrease in the dNTP dissociation rate, suggesting that they perturb Φ29 DNAP interdomain architecture.

WHALE, a management tool for Tier-2 LCG sites

International Nuclear Information System (INIS)

Barone, L M; Organtini, G; Talamo, I G

2012-01-01

The LCG (Worldwide LHC Computing Grid) is a grid-based hierarchical computing distributed facility, composed of more than 140 computing centers, organized in 4 tiers, by size and offer of services. Every site, although indipendent for many technical choices, has to provide services with a well-defined set of interfaces. For this reason, different LCG sites need frequently to manage very similar situations, like jobs behaviour on the batch system, dataset transfers between sites, operating system and experiment software installation and configuration, monitoring of services. In this context we created WHALE (WHALE Handles Administration in an LCG Environment), a software actually used at the T2 I T R ome site, an LCG Tier-2 for the CMS experiment. WHALE is a generic, site independent tool written in Python: it allows administrator to interact in a uniform and coherent way with several subsystems using a high level syntax which hides specific commands. The architecture of WHALE is based on the plugin concept and on the possibility of connecting the output of a plugin to the input of the next one, in a pipe-like system, giving the administrator the possibility of making complex functions by combining the simpler ones. The core of WHALE just handles the plugin orchestrations, while even the basic functions (eg. the WHALE activity logging) are performed by plugins, giving the capability to tune and possibly modify every component of the system. WHALE already provides many plugins useful for a LCG site and some more for a Tier-2 of the CMS experiment, especially in the field of job management, dataset transfer and analysis of performance results and availability tests (eg. Nagios tests, SAM tests). Thanks to its architecture and the provided plugins WHALE makes easy to perform tasks that, even if logically simple, are technically complex or tedious, like eg. closing all the worker nodes with a job-failure rate greater than a given threshold. Finally, thanks to the

The TDAQ Baseline Architecture

CERN Multimedia

Wickens, F J

The Trigger-DAQ community is currently busy preparing material for the DAQ, HLT and DCS TDR. Over the last few weeks a very important step has been a series of meetings to complete agreement on the baseline architecture. An overview of the architecture indicating some of the main parameters is shown in figure 1. As reported at the ATLAS Plenary during the February ATLAS week, the main area where the baseline had not yet been agreed was around the Read-Out System (ROS) and details in the DataFlow. The agreed architecture has: Read-Out Links (ROLs) from the RODs using S-Link; Read-Out Buffers (ROB) sited near the RODs, mounted in a chassis - today assumed to be a PC, using PCI bus at least for configuration, control and monitoring. The baseline assumes data aggregation, in the ROB and/or at the output (which could either be over a bus or in the network). Optimization of the data aggregation will be made in the coming months, but the current model has each ROB card receiving input from 4 ROLs, and 3 such c...

Evalution of BIM and Ecotect for Conceptual Architectural Design Analysis

DEFF Research Database (Denmark)

Thuesen, Niels; Kirkegaard, Poul Henning; Jensen, Rasmus Lund

2010-01-01

The main goal of the present paper is to investigate how BIM tools and Ecotect can be integrated as active part of an integrated design process for conceptual architectural design. The integrated design has an interaction between the skills of the architect and the engineer thought-out the process...... and thereby avoiding problems solving after the design has been finalised. The process has been analysed from an architect's point of view dealing with design at fictive sites in Copenhagen. The results of the research indicate that BIM tools combined with Ecotect can deliver useable qualitative input...

Architectural slicing

DEFF Research Database (Denmark)

Christensen, Henrik Bærbak; Hansen, Klaus Marius

2013-01-01

Architectural prototyping is a widely used practice, con- cerned with taking architectural decisions through experiments with light- weight implementations. However, many architectural decisions are only taken when systems are already (partially) implemented. This is prob- lematic in the context...... of architectural prototyping since experiments with full systems are complex and expensive and thus architectural learn- ing is hindered. In this paper, we propose a novel technique for harvest- ing architectural prototypes from existing systems, \\architectural slic- ing", based on dynamic program slicing. Given...... a system and a slicing criterion, architectural slicing produces an architectural prototype that contain the elements in the architecture that are dependent on the ele- ments in the slicing criterion. Furthermore, we present an initial design and implementation of an architectural slicer for Java....

Telemetric Study of Sleep Architecture and Sleep Homeostasis in the Day-Active Tree Shrew Tupaia belangeri

NARCIS (Netherlands)

Coolen, Alex; Hoffmann, Kerstin; Barf, R. Paulien; Fuchs, Eberhard; Meerlo, Peter

2012-01-01

Study Objectives: In this study the authors characterized sleep architecture and sleep homeostasis in the tree shrew, Tupaia belangeri, a small, omnivorous, day-active mammal that is closely related to primates. Design: Adult tree shrews were individually housed under a 12-hr light/12-hr dark cycle

SUSTAINABLE ARCHITECTURE : WHAT ARCHITECTURE STUDENTS THINK

OpenAIRE

SATWIKO, PRASASTO

2013-01-01

Sustainable architecture has become a hot issue lately as the impacts of climate change become more intense. Architecture educations have responded by integrating knowledge of sustainable design in their curriculum. However, in the real life, new buildings keep coming with designs that completely ignore sustainable principles. This paper discusses the results of two national competitions on sustainable architecture targeted for architecture students (conducted in 2012 and 2013). The results a...

‘Research through designing’ in landscape architecture

NARCIS (Netherlands)

Lenzholzer, S.; Duchhart, I.; Koh, J.

2013-01-01

There is a general consensus amongst landscape architecture academia that the discipline has to urgently advance its methodological repertoire to generate new knowledge and thus strengthen the academic position of landscape architecture. To enhance the methodological repertoire, the core activity of

Architecture

OpenAIRE

Clear, Nic

2014-01-01

When discussing science fiction’s relationship with architecture, the usual practice is to look at the architecture “in” science fiction—in particular, the architecture in SF films (see Kuhn 75-143) since the spaces of literary SF present obvious difficulties as they have to be imagined. In this essay, that relationship will be reversed: I will instead discuss science fiction “in” architecture, mapping out a number of architectural movements and projects that can be viewed explicitly as scien...

Active Sites Environmental Monitoring Program: Mid-FY 1991 report

Energy Technology Data Exchange (ETDEWEB)

Ashwood, T.L.; Wickliff, D.S.; Morrissey, C.M.

1991-10-01

This report summarizes the activities of the Active Sites Environmental Monitoring Program (ASEMP) from October 1990 through March 1991. The ASEMP was established in 1989 by Solid Waste Operations and the Environmental Sciences Division to provide early detection and performance monitoring at active low-level radioactive waste (LLW) disposal sites in Solid Waste Storage Area (SWSA) 6 and transuranic (TRU) waste storage sites in SWSA 5 as required by chapters II and III of US Department of Energy Order 5820.2A. Monitoring results continue to demonstrate the no LLW is being leached from the storage vaults on the tumulus pads. Loading of vaults on Tumulus II began during this reporting period and 115 vaults had been loaded by the end of March 1991.

Molecular dynamics explorations of active site structure in designed and evolved enzymes.

Science.gov (United States)

Osuna, Sílvia; Jiménez-Osés, Gonzalo; Noey, Elizabeth L; Houk, K N

2015-04-21

This Account describes the use of molecular dynamics (MD) simulations to reveal how mutations alter the structure and organization of enzyme active sites. As proposed by Pauling about 70 years ago and elaborated by many others since then, biocatalysis is efficient when functional groups in the active site of an enzyme are in optimal positions for transition state stabilization. Changes in mechanism and covalent interactions are often critical parts of enzyme catalysis. We describe our explorations of the dynamical preorganization of active sites using MD, studying the fluctuations between active and inactive conformations normally concealed to static crystallography. MD shows how the various arrangements of active site residues influence the free energy of the transition state and relates the populations of the catalytic conformational ensemble to the enzyme activity. This Account is organized around three case studies from our laboratory. We first describe the importance of dynamics in evaluating a series of computationally designed and experimentally evolved enzymes for the Kemp elimination, a popular subject in the enzyme design field. We find that the dynamics of the active site is influenced not only by the original sequence design and subsequent mutations but also by the nature of the ligand present in the active site. In the second example, we show how microsecond MD has been used to uncover the role of remote mutations in the active site dynamics and catalysis of a transesterase, LovD. This enzyme was evolved by Tang at UCLA and Codexis, Inc., and is a useful commercial catalyst for the production of the drug simvastatin. X-ray analysis of inactive and active mutants did not reveal differences in the active sites, but relatively long time scale MD in solution showed that the active site of the wild-type enzyme preorganizes only upon binding of the acyl carrier protein (ACP) that delivers the natural acyl group to the active site. In the absence of bound ACP

Open System Architecture design for planet surface systems

Science.gov (United States)

Petri, D. A.; Pieniazek, L. A.; Toups, L. D.

1992-01-01

The Open System Architecture is an approach to meeting the needs for flexibility and evolution of the U.S. Space Exploration Initiative program of the manned exploration of the solar system and its permanent settlement. This paper investigates the issues that future activities of the planet exploration program must confront, defines the basic concepts that provide the basis for establishing an Open System Architecture, identifies the appropriate features of such an architecture, and discusses examples of Open System Architectures.

Klipperaas study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Andersson, Peter; Ittner, T.; Tiren, S.; Ljunggren, C.

1992-09-01

During the period from 1977 - 1986 SKB (Swedish Nuclear Fuel and Waste Management Co.) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection rpogramme will be to perform detailed characterisation, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if any of the study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favorable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concern the Klipperaas study site. The main topics are the scope of activities, geologic model, geohydrological model, groundwater chemistry, assessment of solute transport, and rock mechanics

Active Sites Environmental Monitoring Program: FY 1990 annual report

International Nuclear Information System (INIS)

Wickliff, D.S.; Morrissey, C.M.; Ashwood, T.L.

1991-10-01

Chapter 3 of US Department of Energy (DOE) Order 5820.2A (DOE 1988) sets forth requirements for environmental monitoring of active low-level waste (LLW) disposal sites. Active sites are defined as those LLW facilities that were in use on or after the date of the order (September 1988). The transuranic (TRU) waste storage areas in Solid Waste Storage Area (SWSA) 5 North are covered by Chap. 2 of the order. In both chapters, monitoring is required to provide for early warning of leaks before those leaks pose a threat to human health or the environment. Chapter 3 also requires that monitoring be conducted to evaluate the short- and long-term performance of LLW disposal facilities. In accordance with this order, the Solid Waste Operations Department at Oak Ridge National Laboratory (ORNL) has established an Active Sites Environmental Monitoring Program (ASEMP) that is implemented by staff of the Environmental Sciences Division (ESD) at ORNL. This report summarizes data from ASEMP monitoring activities for the final 6 months of FY 1990. A brief summary of the monitoring methodology for each site is presented also

Architecture in motion: A model for music composition

Science.gov (United States)

Variego, Jorge Elias

2011-12-01

Speculations regarding the relationship between music and architecture go back to the very origins of these disciplines. Throughout history, these links have always reaffirmed that music and architecture are analogous art forms that only diverge in their object of study. In the 1 st c. BCE Vitruvius conceived Architecture as "one of the most inclusive and universal human activities" where the architect should be educated in all the arts, having a vast knowledge in history, music and philosophy. In the 18th c., the German thinker Johann Wolfgang von Goethe, described Architecture as "frozen music". More recently, in the 20th c., Iannis Xenakis studied the similar structuring principles between Music and Architecture creating his own "models" of musical composition based on mathematical principles and geometric constructions. The goal of this document is to propose a compositional method that will function as a translator between the acoustical properties of a room and music, to facilitate the creation of musical works that will not only happen within an enclosed space but will also intentionally interact with the space. Acoustical measurements of rooms such as reverberation time, frequency response and volume will be measured and systematically organized in correspondence with orchestrational parameters. The musical compositions created after the proposed model are evocative of the spaces on which they are based. They are meant to be performed in any space, not exclusively in the one where the acoustical measurements were obtained. The visual component of architectural design is disregarded; the room is considered a musical instrument, with its particular sound qualities and resonances. Compositions using the proposed model will not result as sonified shapes, they will be musical works literally "tuned" to a specific space. This Architecture in motion is an attempt to adopt scientific research to the service of a creative activity and to let the aural properties of

Kosmos = architecture

Directory of Open Access Journals (Sweden)

Tine Kurent

1985-12-01

Full Text Available The old Greek word "kosmos" means not only "cosmos", but also "the beautiful order", "the way of building", "building", "scenography", "mankind", and, in the time of the New Testament, also "pagans". The word "arhitekton", meaning first the "master of theatrical scenography", acquired the meaning of "builder", when the words "kosmos" and ~kosmetes" became pejorative. The fear that architecture was not considered one of the arts before Renaissance, since none of the Muses supervised the art of building, results from the misunderstanding of the word "kosmos". Urania was the Goddes of the activity implied in the verb "kosmein", meaning "to put in the beautiful order" - everything, from the universe to the man-made space, i. e. the architecture.

Public regulations towards a tectonic architecture

DEFF Research Database (Denmark)

Schmidt, Anne Marie Due

2006-01-01

's activities has primarily been to support the optimization of the building process through ‘trimmed building’ and ‘partnering’ that only takes the immediate economic benefits of the changes to the building process into account and as such has no measures for architectural quality. The public initiatives so......Public regulations can support tectonic architecture by changes to the tendering system, supporting new organizational structures of the building industry in public building projects and suggesting a focus on innovation through increased research and development activity. The Danish state...... are happening very slowly which is understandable when there is no economic incitement for the industry to change. A change of these public regulations from sticks to carrots could create the economic incitement for the building industry to create tectonic architecture and thereby develop the building industry...

Modeling Architectural Patterns Using Architectural Primitives

NARCIS (Netherlands)

Zdun, Uwe; Avgeriou, Paris

2005-01-01

Architectural patterns are a key point in architectural documentation. Regrettably, there is poor support for modeling architectural patterns, because the pattern elements are not directly matched by elements in modeling languages, and, at the same time, patterns support an inherent variability that

Tritium activities in selected wells on the Nevada Test Site

International Nuclear Information System (INIS)

Lyles, B.F.

1993-05-01

Literature and data were reviewed related to radionuclides in groundwater on and near the Nevada Test Site. No elevated tritium activities have been reported outside of the major testing regions of the Nevada Test Site. Three wells were identified as having water with above-background (>50 pCi/l) tritium activities: UE-15d Water Well; USGS Water Well A; and USGS Test Well B Ex. Although none of these wells have tritium activities greater than the Nevada State Drinking Water standard (20,000 pCi/l), their time-series tritium trends may be indicative to potential on-site radionuclide migration

Development of METAL-ACTIVE SITE and ZINCCLUSTER tool to predict active site pockets.

Science.gov (United States)

Ajitha, M; Sundar, K; Arul Mugilan, S; Arumugam, S

2018-03-01

The advent of whole genome sequencing leads to increasing number of proteins with known amino acid sequences. Despite many efforts, the number of proteins with resolved three dimensional structures is still low. One of the challenging tasks the structural biologists face is the prediction of the interaction of metal ion with any protein for which the structure is unknown. Based on the information available in Protein Data Bank, a site (METALACTIVE INTERACTION) has been generated which displays information for significant high preferential and low-preferential combination of endogenous ligands for 49 metal ions. User can also gain information about the residues present in the first and second coordination sphere as it plays a major role in maintaining the structure and function of metalloproteins in biological system. In this paper, a novel computational tool (ZINCCLUSTER) is developed, which can predict the zinc metal binding sites of proteins even if only the primary sequence is known. The purpose of this tool is to predict the active site cluster of an uncharacterized protein based on its primary sequence or a 3D structure. The tool can predict amino acids interacting with a metal or vice versa. This tool is based on the occurrence of significant triplets and it is tested to have higher prediction accuracy when compared to that of other available techniques. © 2017 Wiley Periodicals, Inc.

Zymogen Activation and Subcellular Activity of Subtilisin Kexin Isozyme 1/Site 1 Protease*

Science.gov (United States)

da Palma, Joel Ramos; Burri, Dominique Julien; Oppliger, Joël; Salamina, Marco; Cendron, Laura; de Laureto, Patrizia Polverino; Seidah, Nabil Georges; Kunz, Stefan; Pasquato, Antonella

2014-01-01

The proprotein convertase subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P) plays crucial roles in cellular homeostatic functions and is hijacked by pathogenic viruses for the processing of their envelope glycoproteins. Zymogen activation of SKI-1/S1P involves sequential autocatalytic processing of its N-terminal prodomain at sites B′/B followed by the herein newly identified C′/C sites. We found that SKI-1/S1P autoprocessing results in intermediates whose catalytic domain remains associated with prodomain fragments of different lengths. In contrast to other zymogen proprotein convertases, all incompletely matured intermediates of SKI-1/S1P showed full catalytic activity toward cellular substrates, whereas optimal cleavage of viral glycoproteins depended on B′/B processing. Incompletely matured forms of SKI-1/S1P further process cellular and viral substrates in distinct subcellular compartments. Using a cell-based sensor for SKI-1/S1P activity, we found that 9 amino acid residues at the cleavage site (P1–P8) and P1′ are necessary and sufficient to define the subcellular location of processing and to determine to what extent processing of a substrate depends on SKI-1/S1P maturation. In sum, our study reveals novel and unexpected features of SKI-1/S1P zymogen activation and subcellular specificity of activity toward cellular and pathogen-derived substrates. PMID:25378398

Sites of flux: imagining space in the dance-architectures of The Changing Room and Sea Unsea

DEFF Research Database (Denmark)

Ramsgaard Thomsen, Mette

2008-01-01

contemporary architectural concepts of space and inhabitation can allow for a new framing of interactive experiences. Presenting the two dance-architectures The Changing Room and Sea Unsea as case studies, the paper seeks to demonstrate challenges to the way space and place are understood in Human...

Brain architecture: a design for natural computation.

Science.gov (United States)

Kaiser, Marcus

2007-12-15

Fifty years ago, John von Neumann compared the architecture of the brain with that of the computers he invented and which are still in use today. In those days, the organization of computers was based on concepts of brain organization. Here, we give an update on current results on the global organization of neural systems. For neural systems, we outline how the spatial and topological architecture of neuronal and cortical networks facilitates robustness against failures, fast processing and balanced network activation. Finally, we discuss mechanisms of self-organization for such architectures. After all, the organization of the brain might again inspire computer architecture.

Active sites environmental monitoring program FY 1997 annual report

International Nuclear Information System (INIS)

Morrissey, C.M.; Marshall, D.S.; Cunningham, G.R.

1998-03-01

This report summarizes the activities conducted by the Active Sites Environmental Monitoring Program (ASEMP) from October 1996 through September 1997. The purpose of the program is to provide early detection and performance monitoring at active low-level waste (LLW) disposal sites in Solid Waste Storage Area (SWSA) 6 and transuranic (TRU) waste storage sites in SWSA 5 North. This report continues a series of annual and semiannual reports that present the results of ASEMP monitoring activities. This report details monitoring results for fiscal year (FY) 1997 from SWSA 6, including the Interim Waste Management Facility (IWMF) and the Hillcut Disposal Test Facility (HDTF), and (2) TRU-waste storage areas in SWSA 5 N. This report presents a summary of the methodology used to gather data for each major area along with the FY 1997 results. Figures referenced in the text are found in Appendix A and data tables are presented in Appendix B

ORNAMENTAL ART AND SYMBOLISM: ACTIVATORS OF HISTORICAL REGENERATION FOR KAZAKHSTAN’S LANDSCAPE ARCHITECTURE

Directory of Open Access Journals (Sweden)

Akmaral Ardasher Yussupova

2017-11-01

Full Text Available The use of symbolism in contemporary architecture is increasingly gaining momentum, especially so in the Eastern countries currently undergoing rapid economic development. Sociologically, this phenomenon can be related to a desire to manifest a vast wealth of national art and respond to the globalisation and unification of world culture. Taking this tendency as a prompt, this study explores different ways of implementing symbolic ornaments in landscape architecture. Traditionally architecture has been defined through and judged against culturally acceptable criteria that set the norm for appropriate form and expression. Yet, technical advances have altered this process and contributed to a certain level of oblivion of traditional architectural form. Thus, the meaning of many Kazakh ornaments has been lost through time. On one hand, this paper collects historical information on the semiotics of Kazakh ornaments and on the other hand, it conducts field studies focusing on the cultural tradition of the native people in Eurasia. The study introduces the use of symbolism in landscape architecture as an aspiration for luck and prosperity which then dictates the quality of the landscape compositions. The findings show that the use of symbolic ornamentation in architecture is not bound to specific geographic areas but rather motivated by broader underlying principles. Through analytical exploration of different cultures and their use of symbols in architecture, this study identifies four main categories of architectural symbolism relating to floral, zoomorphic, geometric and cosmogonic patterns. Each nation then recognises its own identity in the semiotics of those patterns and incorporates them in the urban realm as part of its cultural legacy.

Mitofilin complexes : conserved organizers of mitochondrial membrane architecture

NARCIS (Netherlands)

Zerbes, Ralf M.; van der Klei, Ida J.; Veenhuis, Marten; Pfanner, Nikolaus; van der Laan, Martin; Bohnert, Maria

2012-01-01

Mitofilin proteins are crucial organizers of mitochondrial architecture. They are located in the inner mitochondrial membrane and interact with several protein complexes of the outer membrane, thereby generating contact sites between the two membrane systems of mitochondria. Within the inner

The brand architecture of grocery retailers

DEFF Research Database (Denmark)

Bech-Larsen, Tino; Esbjerg, Lars

2009-01-01

This article discusses how the brand architecture of grocery retailers set material and symbolic boundaries for consumer choice, thus limiting consumer sovereignty. The article first discusses previous work on store atmospherics, servicescapes and brand architecture. It is argued that work based...... on these concepts has taken an internal management perspective on how retailers can manipulate aspects of the retail setting to serve their own interests. Then, we develop an alternative conceptualisation of retailer brand architecture that takes into account that consumers (and other constituents) are active co......- constructors of material and symbolic aspects of retail settings. It is discussed how consumers participate in constructing retailer brand architecture and how this concept differs from previous research. Implications for both research and practice are discussed....

Monitoring WLCG with lambda-architecture: a new scalable data store and analytics platform for monitoring at petabyte scale.

CERN Document Server

Magnoni, L; Cordeiro, C; Georgiou, M; Andreeva, J; Khan, A; Smith, D R

2015-01-01

Monitoring the WLCG infrastructure requires the gathering and analysis of a high volume of heterogeneous data (e.g. data transfers, job monitoring, site tests) coming from different services and experiment-specific frameworks to provide a uniform and flexible interface for scientists and sites. The current architecture, where relational database systems are used to store, to process and to serve monitoring data, has limitations in coping with the foreseen increase in the volume (e.g. higher LHC luminosity) and the variety (e.g. new data-transfer protocols and new resource-types, as cloud-computing) of WLCG monitoring events. This paper presents a new scalable data store and analytics platform designed by the Support for Distributed Computing (SDC) group, at the CERN IT department, which uses a variety of technologies each one targeting specific aspects of big-scale distributed data-processing (commonly referred as lambda-architecture approach). Results of data processing on Hadoop for WLCG data activities mon...

Architecture as mediator of culture, democracy and hope: XXIII World Congress of Architecture in Turin

Directory of Open Access Journals (Sweden)

Bogdanov Ana

2009-01-01

Full Text Available The XXIII World Congress of Architecture organized by The International Union of Architects (UIA was held in Turin from June 29 to July 03. Every third year this great architectural event gathers together more than one thousand professionals and students from 126 countries that UIA encompasses today. The main topic of the this year Congress was 'Transmitting Architecture' suggesting that future architecture will increasingly depend on communication and integration. It also suggests that global problems which architecture has been encountered with in recent years should be considered at all levels through ever active network of information and communications. In that term exchanging and sharing knowledge and experience among architects of different national and social backgrounds are crucial. Besides, transmitting architecture has also a faint futuristic sound which points out new technologies and their increasing importance in architecture. At last, this topic emphasize that the Congress has been oriented towards and open to not only academic discussions, but to younger generations and students who have always been most experienced in using digital technologies. Throughout the Congress Programme which consisted of lectures, debates, workshops exhibitions, where more than 600 speakers participated, various topics were discussed with a common aim to gain an insight into the situation on current architectural scene, to realize the position of architects and architecture in today's sphere of social, political, cultural, technical and technological factors, and consequently to establish possible tracks and offer recommendations for further progress in this field. Out of Congress, the participants had the opportunity of being introduced to Turin, the city of vision and sustainability put into action.

Web based aphasia test using service oriented architecture (SOA)

International Nuclear Information System (INIS)

Voos, J A; Vigliecca, N S; Gonzalez, E A

2007-01-01

Based on an aphasia test for Spanish speakers which analyze the patient's basic resources of verbal communication, a web-enabled software was developed to automate its execution. A clinical database was designed as a complement, in order to evaluate the antecedents (risk factors, pharmacological and medical backgrounds, neurological or psychiatric symptoms, brain injury -anatomical and physiological characteristics, etc) which are necessary to carry out a multi-factor statistical analysis in different samples of patients. The automated test was developed following service oriented architecture and implemented in a web site which contains a tests suite, which would allow both integrating the aphasia test with other neuropsychological instruments and increasing the available site information for scientific research. The test design, the database and the study of its psychometric properties (validity, reliability and objectivity) were made in conjunction with neuropsychological researchers, who participate actively in the software design, based on the patients or other subjects of investigation feedback

Web based aphasia test using service oriented architecture (SOA)

Energy Technology Data Exchange (ETDEWEB)

Voos, J A [Clinical Engineering R and D Center, Universidad Tecnologica Nacional, Facultad Regional Cordoba, Cordoba (Argentina); Vigliecca, N S [Consejo Nacional de Investigaciones Cientificas y Tecnicas, CONICET, Cordoba (Argentina); Gonzalez, E A [Clinical Engineering R and D Center, Universidad Tecnologica Nacional, Facultad Regional Cordoba, Cordoba (Argentina)

2007-11-15

Based on an aphasia test for Spanish speakers which analyze the patient's basic resources of verbal communication, a web-enabled software was developed to automate its execution. A clinical database was designed as a complement, in order to evaluate the antecedents (risk factors, pharmacological and medical backgrounds, neurological or psychiatric symptoms, brain injury -anatomical and physiological characteristics, etc) which are necessary to carry out a multi-factor statistical analysis in different samples of patients. The automated test was developed following service oriented architecture and implemented in a web site which contains a tests suite, which would allow both integrating the aphasia test with other neuropsychological instruments and increasing the available site information for scientific research. The test design, the database and the study of its psychometric properties (validity, reliability and objectivity) were made in conjunction with neuropsychological researchers, who participate actively in the software design, based on the patients or other subjects of investigation feedback.

Active site loop conformation regulates promiscuous activity in a lactonase from Geobacillus kaustophilus HTA426.

Directory of Open Access Journals (Sweden)

Yu Zhang

Full Text Available Enzyme promiscuity is a prerequisite for fast divergent evolution of biocatalysts. A phosphotriesterase-like lactonase (PLL from Geobacillus kaustophilus HTA426 (GkaP exhibits main lactonase and promiscuous phosphotriesterase activities. To understand its catalytic and evolutionary mechanisms, we investigated a "hot spot" in the active site by saturation mutagenesis as well as X-ray crystallographic analyses. We found that position 99 in the active site was involved in substrate discrimination. One mutant, Y99L, exhibited 11-fold improvement over wild-type in reactivity (kcat/Km toward the phosphotriesterase substrate ethyl-paraoxon, but showed 15-fold decrease toward the lactonase substrate δ-decanolactone, resulting in a 157-fold inversion of the substrate specificity. Structural analysis of Y99L revealed that the mutation causes a ∼6.6 Å outward shift of adjacent loop 7, which may cause increased flexibility of the active site and facilitate accommodation and/or catalysis of organophosphate substrate. This study provides for the PLL family an example of how the evolutionary route from promiscuity to specificity can derive from very few mutations, which promotes alteration in the conformational adjustment of the active site loops, in turn draws the capacity of substrate binding and activity.

Parametric Approach in Designing Large-Scale Urban Architectural Objects

Directory of Open Access Journals (Sweden)

Arne Riekstiņš

2011-04-01

Full Text Available When all the disciplines of various science fields converge and develop, new approaches to contemporary architecture arise. The author looks towards approaching digital architecture from parametric viewpoint, revealing its generative capacity, originating from the fields of aeronautical, naval, automobile and product-design industries. The author also goes explicitly through his design cycle workflow for testing the latest methodologies in architectural design. The design process steps involved: extrapolating valuable statistical data about the site into three-dimensional diagrams, defining certain materiality of what is being produced, ways of presenting structural skin and structure simultaneously, contacting the object with the ground, interior program definition of the building with floors and possible spaces, logic of fabrication, CNC milling of the proto-type. The author’s developed tool that is reviewed in this article features enormous performative capacity and is applicable to various architectural design scales.Article in English

Geometrical objects architecture and the mathematical sciences 1400-1800

CERN Document Server

2014-01-01

This volume explores the mathematical character of architectural practice in diverse pre- and early modern contexts. It takes an explicitly interdisciplinary approach, which unites scholarship in early modern architecture with recent work in the history of science, in particular, on the role of practice in the scientific revolution. As a contribution to architectural history, the volume contextualizes design and construction in terms of contemporary mathematical knowledge, attendant forms of mathematical practice, and relevant social distinctions between the mathematical professions. As a contribution to the history of science, the volume presents a series of micro-historical studies that highlight issues of process, materiality, and knowledge production in specific, situated, practical contexts. Our approach sees the designer’s studio, the stone-yard, the drawing floor, and construction site not merely as places where the architectural object takes shape, but where mathematical knowledge itself is depl...

Publicity and identity in the public space architecture

Directory of Open Access Journals (Sweden)

Maria de Lourdes Carneiro da Cunha Nóbrega

2009-12-01

Full Text Available This article aims at showing the relationship between publicity elements, such as posters and signs, with the city architecture, so that the contribution of these elements with the identity of the urban sites, especially the historical ones, can be understood. In order to do that, a focal point is given to Rua da Palma, located in the city of Recife (Pernambuco, Brazil. For the development of this research, which was based on the present morphological analysis of the site, a survey of photographs and the use of the buildings in the street from 2006 to 2009 was carried out. Also, the existing urban legislation was analyzed. Studies undertaken by authors such as Certau (1994, Venturi (1977 and Koolhaas (2004, among others, and concepts related to retail marketing helped in the conclusion of this analysis of the urban space, which presents architecture as a publicity media, often transforming the identity of the area. A starting point is presented here for future investigation on the role of urban laws and urban control, which deal with the placement of publicity elements in the architecture of the city and contribute for the formation or the urban landscape. This landscape which is considered an integral part of a cultural identity.

RESILIENCE AND ENTERPRISE ARCHITECTURE IN SMES

Directory of Open Access Journals (Sweden)

Rui Gomes

2015-12-01

Full Text Available Considering that SMEs need to embrace the drivers of resilience and that a well-defined and readily available Enterprise Architecture (EA supports enterprise integration by enabling the common view of business processes, data and systems across the enterprise and its partners, we can say that EA is one of the tracks making resilience predictable and it should support and collaborate with other resilience tracks. However, the EA frameworks do not give relevance to the activities that contribute most to business resilience, so this paper aims to clarify the dimensions and the activities related to the development of an EA and the touching points with other enterprise wide processes in order to guarantee that resilience requirements are met in SMEs. For this I propose an approach of ecological adaptation, and four architectures: business, organizational, information, and technological, although this paper only presents the Business and Organizational Architectures.

Disulfide bond within mu-calpain active site inhibits activity and autolysis.

Science.gov (United States)

Lametsch, René; Lonergan, Steven; Huff-Lonergan, Elisabeth

2008-09-01

Oxidative processes have the ability to influence mu-calpain activity. In the present study the influence of oxidation on activity and autolysis of mu-calpain was examined. Furthermore, LC-MS/MS analysis was employed to identify and characterize protein modifications caused by oxidation. The results revealed that the activity of mu-calpain is diminished by oxidation with H2O2 in a reversible manner involving cysteine and that the rate of autolysis of mu-calpain concomitantly slowed. The LC-MS/MS analysis of the oxidized mu-calpain revealed that the amino acid residues 105-133 contained a disulfide bond between Cys(108) and Cys(115). The finding that the active site cysteine in mu-calpain is able to form a disulfide bond has, to our knowledge, not been reported before. This could be part of a unique oxidation mechanism for mu-calpain. The results also showed that the formation of the disulfide bond is limited in the control (no oxidant added), and further limited in a concentration-dependent manner when beta-mercaptoethanol is added. However, the disulfide bond is still present to some extent in all conditions indicating that the active site cysteine is potentially highly susceptible to the formation of this intramolecular disulfide bond.

Architectural plasticity in young Eucalyptus marginata on restored bauxite mines and adjacent natural forest in south-western Australia.

Science.gov (United States)

Bleby, Timothy M; Colquhoun, Ian J; Adams, Mark A

2009-08-01

The aboveground architecture of Eucalyptus marginata (Jarrah) was investigated in chronosequences of young trees (2.5, 5 and 10 m height) growing in a seasonally dry climate in a natural forest environment with intact soils, and on adjacent restored bauxite mine sites on soils with highly modified A and B horizons above an intact C horizon. Compared to forest trees, trees on restored sites were much younger and faster growing, with straighter, more clearly defined main stems and deeper, narrower crowns containing a greater number of branches that were longer, thinner and more vertically angled. Trees on restored sites also had a higher fraction of biomass in leaves than forest trees, as indicated by 20-25% thicker leaves, 30-70% greater leaf area, 10-30% greater leaf area to sapwood area ratios and 5-30% lesser branch Huber values. Differences in crown architecture and biomass distribution were consistent with putatively greater soil-water, nutrient and light availability on restored sites. Our results demonstrate that under the same climatic conditions, E. marginata displays a high degree of plasticity of aboveground architecture in response to the net effects of resource availability and soil environment. These differences in architecture are likely to have functional consequences in relation to tree hydraulics and growth that, on larger scales, is likely to affect the water and carbon balances of restored forest ecosystems. This study highlights substrate as a significant determinant of tree architecture in water-limited environments. It further suggests that the architecture of young trees on restored sites may need to change again if they are to survive likely longer-term changes in resource availability.

Architectural prototyping

DEFF Research Database (Denmark)

Bardram, Jakob Eyvind; Christensen, Henrik Bærbak; Hansen, Klaus Marius

2004-01-01

A major part of software architecture design is learning how specific architectural designs balance the concerns of stakeholders. We explore the notion of "architectural prototypes", correspondingly architectural prototyping, as a means of using executable prototypes to investigate stakeholders...

L´Architecture Sauvage

DEFF Research Database (Denmark)

Baumeister, Ruth

Danish artist Asger Jorn (1914-1973) is internationally renowed as a proponent of Nordic art and for his activities as a member of Cobra and the Situationist International. Quite apart from his art, Jorn produced a remarkable quantity of writing on art, architecture and urbanism and engaged in di...

Neural codes of seeing architectural styles.

Science.gov (United States)

Choo, Heeyoung; Nasar, Jack L; Nikrahei, Bardia; Walther, Dirk B

2017-01-10

Images of iconic buildings, such as the CN Tower, instantly transport us to specific places, such as Toronto. Despite the substantial impact of architectural design on people's visual experience of built environments, we know little about its neural representation in the human brain. In the present study, we have found patterns of neural activity associated with specific architectural styles in several high-level visual brain regions, but not in primary visual cortex (V1). This finding suggests that the neural correlates of the visual perception of architectural styles stem from style-specific complex visual structure beyond the simple features computed in V1. Surprisingly, the network of brain regions representing architectural styles included the fusiform face area (FFA) in addition to several scene-selective regions. Hierarchical clustering of error patterns further revealed that the FFA participated to a much larger extent in the neural encoding of architectural styles than entry-level scene categories. We conclude that the FFA is involved in fine-grained neural encoding of scenes at a subordinate-level, in our case, architectural styles of buildings. This study for the first time shows how the human visual system encodes visual aspects of architecture, one of the predominant and longest-lasting artefacts of human culture.

Developer Stories: Improving Architecture in Agile Practice

DEFF Research Database (Denmark)

Tjørnehøj, Gitte; Jensen, Rolf Njor; Platz, Niels

2007-01-01

of viable architectures. To strengthen XP in this regard a new practice: Developer Stories ([2]) was introduced in 2006 - mainly based on a theoretical argumentation. This paper reports from extensive experimentation with, and elaboration of the new practice. Results from this experimentation shows...... that using Developer Stories increases the likelihood of developing a viable architecture through a series of deliberate choices, through creating disciplined and recurring activities that: 1) Facilitate sharing and embodying of knowledge about architectural issues, and 2) heighten visibility of refactorings...

Architectural History and Painting Art at Ajanta: Some Salient Features

Directory of Open Access Journals (Sweden)

Manager Singh

2013-08-01

Full Text Available The present paper deals with the period of India’s greatest cultural fluorescence, The Golden Age. A lively intellectual debate has been going on among historians and archaeologists on the subject of the chronology of the caves at Ajanta. The study shows addition of many iconographic styles from Ajanta to Bagh caves and vice-versa due to movement of skilled workers and craftsmen between these two Buddhist sites. Many iconographic figures were added / deleted depending on the local condition of rock at both the sites. The art and architecture of these sites reflects India’s great materials culture. The Ajanta paintings are not just a milestone in the history of development of world art but they also convey unique insights about the life of ancient Indians and their culture. This paper outlines some of the architectural features introduced from Ajanta into the other cultures. Furthermore, structural conservation measures and scientific methodology adopted for the preservation of Ajanta murals have also been highlighted.

A method for valuing architecture-based business transformation and measuring the value of solutions architecture

NARCIS (Netherlands)

Slot, R.G.

2010-01-01

Enterprise and Solution Architecture are key in today’s business environment. It is surprising that the foundation and business case for these activities are nonexistent; the financial value for the business of these activities is largely undetermined. To determine business value of enterprise and

Anisotropic Covalency Contributions to Superexchange Pathways in Type One Copper Active Sites

Science.gov (United States)

2015-01-01

Type one (T1) Cu sites deliver electrons to catalytic Cu active sites: the mononuclear type two (T2) Cu site in nitrite reductases (NiRs) and the trinuclear Cu cluster in the multicopper oxidases (MCOs). The T1 Cu and the remote catalytic sites are connected via a Cys-His intramolecular electron-transfer (ET) bridge, which contains two potential ET pathways: P1 through the protein backbone and P2 through the H-bond between the Cys and the His. The high covalency of the T1 Cu–S(Cys) bond is shown here to activate the T1 Cu site for hole superexchange via occupied valence orbitals of the bridge. This covalency-activated electronic coupling (HDA) facilitates long-range ET through both pathways. These pathways can be selectively activated depending on the geometric and electronic structure of the T1 Cu site and thus the anisotropic covalency of the T1 Cu–S(Cys) bond. In NiRs, blue (π-type) T1 sites utilize P1 and green (σ-type) T1 sites utilize P2, with P2 being more efficient. Comparing the MCOs to NiRs, the second-sphere environment changes the conformation of the Cys-His pathway, which selectively activates HDA for superexchange by blue π sites for efficient turnover in catalysis. These studies show that a given protein bridge, here Cys-His, provides different superexchange pathways and electronic couplings depending on the anisotropic covalencies of the donor and acceptor metal sites. PMID:25310460

Module Architecture for in Situ Space Laboratories

Science.gov (United States)

Sherwood, Brent

2010-01-01

The paper analyzes internal outfitting architectures for space exploration laboratory modules. ISS laboratory architecture is examined as a baseline for comparison; applicable insights are derived. Laboratory functional programs are defined for seven planet-surface knowledge domains. Necessary and value-added departures from the ISS architecture standard are defined, and three sectional interior architecture options are assessed for practicality and potential performance. Contemporary guidelines for terrestrial analytical laboratory design are found to be applicable to the in-space functional program. Densepacked racks of system equipment, and high module volume packing ratios, should not be assumed as the default solution for exploration laboratories whose primary activities include un-scriptable investigations and experimentation on the system equipment itself.

Architectural freedom and industrialized architecture

DEFF Research Database (Denmark)

Vestergaard, Inge

2012-01-01

to explain that architecture can be thought as a complex and diverse design through customization, telling exactly the revitalized storey about the change to a contemporary sustainable and better performing expression in direct relation to the given context. Through the last couple of years we have...... expression in the specific housing area. It is the aim of this article to expand the different design strategies which architects can use – to give the individual project attitudes and designs with architectural quality. Through the customized component production it is possible to choose different...... for retrofit design. If we add the question of the installations e.g. ventilation to this systematic thinking of building technique we get a diverse and functional architecture, thereby creating a new and clearer story telling about new and smart system based thinking behind architectural expression....

Architectural freedom and industrialised architecture

DEFF Research Database (Denmark)

Vestergaard, Inge

2012-01-01

Architectural freedom and industrialized architecture. Inge Vestergaard, Associate Professor, Cand. Arch. Aarhus School of Architecture, Denmark Noerreport 20, 8000 Aarhus C Telephone +45 89 36 0000 E-mai l inge.vestergaard@aarch.dk Based on the repetitive architecture from the "building boom" 1960...... customization, telling exactly the revitalized storey about the change to a contemporary sustainable and better performed expression in direct relation to the given context. Through the last couple of years we have in Denmark been focusing a more sustainable and low energy building technique, which also include...... to the building physic problems a new industrialized period has started based on light weight elements basically made of wooden structures, faced with different suitable materials meant for individual expression for the specific housing area. It is the purpose of this article to widen up the different design...

Documenting Modern Mexican Architectural Heritage for Posterity: Barragan's Casa Cristo, in Guadalajara, Mexico

Science.gov (United States)

Mezzino, D.; Pei, W.; Santana Quintero, M.; Reyes Rodriguez, R.

2015-08-01

This contribution describes the results of an International workshop on documentation of historic and cultural heritage developed jointly between Universidad de Guadalajara's Centro Universitario de Arte, Arquitectura y Diseño (CUAAD) and Carleton University's Architectural Conservation and Sustainability Program. The objective of the workshop was to create a learning environment for emerging heritage professionals through the use of advanced recording techniques for the documentation of modern architectural heritage in Guadalajara, Mexico. The selected site was Casa Cristo, one of the several architectural projects by Luis Barragán in Guadalajara. The house was built between 1927 and 1929 for Gustavo R. Cristo, mayor of the city. The style of the building reflects the European influences derived from the architect's travel experience, as well as the close connection with local craftsmanship. All of these make the house an outstanding example of modern regional architecture. A systematic documentation strategy was developed for the site, using different survey equipment and techniques to capture the shape, colour, spatial configuration, and current conditions of Casa Cristo for its eventual rehabilitation and conservation.

Architecture and memory

Directory of Open Access Journals (Sweden)

Eneida de Almeida

2015-12-01

Full Text Available This paper investigates the links between architecture design and restoration, considering the blurry frontier that distinguishes this actions. The study holds in two contemporary architects performance: Lina Bo Bardi (1914-1992 and Aldo Rossi (1931-1997. The analyses of the concrete production, presented here by a work of each architecture – Sesc Pompeia and the Teatro Del Mondo – is based on the ability of reflection on the role of the memory in architecture: not only the memory in the buildings and urban fabrics materiality, but also the memory as an active instrument inside the mental processes adopted by the projects authors. Resorting to architects writings as well as authors who analyses this interventions, they seek to reconstitute the design development path, recognizing the strategy that reinterprets past experiences in order to overcome the traditional contraposition between “old” and “new”, tutorship and innovation.

Conservation of local architecture from primary education

Directory of Open Access Journals (Sweden)

Paula Esther Azcuy Chiroles

2005-12-01

Full Text Available The knowledge of the local architecture in the primary school is insufficient, according to results of the diagnosis carried out for the investigation of the Project of Local Art of the Artistic Education Department of ISP “Rafael M. Mendive.” The lack of systematic work was demonstrated in the promotion of local architecture starting from the subject of Plastic arts, in its curricular and extracurricular components. This is the reason for presenting an Strategy for the promotion of the local architecture in primary teaching, with a System of Activities for the promotion of the local civil architecture that allows the motivation of the students, professors, family and Institutions toward the acquisition of knowledge on the local artistic culture, and the appreciation of the most illustrative architectural patrimonial values in Pinar del Río city.

Site characterization techniques used in environmental remediation activities

International Nuclear Information System (INIS)

Kostelnik, K.M.

2000-01-01

As a result of decades of nuclear energy research, weapons production, as well as ongoing operations, a significant amount of radioactive contamination has occurred throughout the United States Department of Energy (DOE) complex. DOE facility are in the process of assessing and potentially remediating various sites according to the regulations imposed by a Federal Facility Agreement and Consent order (FFA/CO) between DOE, the state in which the facility is located, and the U.S. Environmental Protection Agency (EPA). In support of these active site remediation efforts, the DOE has devoted considerable resources towards the development of innovative site characterization techniques that support environmental restoration activities. These resources and efforts have focused on various aspects of this complex problem. Research and technology development conducted at the Idaho National Engineering and Environmental Laboratory (INEEL) has resulted in the ability and state-of-the-art equipment required to obtain real-time, densely spaced, in situ characterization data (i.e. detection, speciation, and location) of various radionuclides and contaminants. The Remedial Action Monitoring System (RAMS), developed by the INEEL, consists of enhanced sensor technology, measurement modeling and interpretation techniques, and a suite of deployment platforms which can be interchanged to directly support remedial cleanup and site verification operations. In situ characterization techniques have advanced to the point where they are being actively deployed in support of remedial operations. The INEEL has deployed its system at various DOE and international sites. The deployment of in situ characterization systems during environmental restoration operations has shown that this approach results in several significant benefits versus conventional sampling techniques. A flexible characterization system permits rapid modification to satisfy physical site conditions, available site resources

Architecture for large-scale automatic web accessibility evaluation based on the UWEM methodology

DEFF Research Database (Denmark)

Ulltveit-Moe, Nils; Olsen, Morten Goodwin; Pillai, Anand B.

2008-01-01

The European Internet Accessibility project (EIAO) has developed an Observatory for performing large scale automatic web accessibility evaluations of public sector web sites in Europe. The architecture includes a distributed web crawler that crawls web sites for links until either a given budget...... of web pages have been identified or the web site has been crawled exhaustively. Subsequently, a uniform random subset of the crawled web pages is sampled and sent for accessibility evaluation and the evaluation results are stored in a Resource Description Format (RDF) database that is later loaded...... challenges that the project faced and the solutions developed towards building a system capable of regular large-scale accessibility evaluations with sufficient capacity and stability. It also outlines some possible future architectural improvements....

Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination

OpenAIRE

Rowley, Paul A.; Kachroo, Aashiq H.; Ma, Chien-Hui; Maciaszek, Anna D.; Guga, Piotr; Jayaram, Makkuni

2015-01-01

Tyrosine site-specific recombinases, which promote one class of biologically important phosphoryl transfer reactions in DNA, exemplify active site mechanisms for stabilizing the phosphate transition state. A highly conserved arginine duo (Arg-I; Arg-II) of the recombinase active site plays a crucial role in this function. Cre and Flp recombinase mutants lacking either arginine can be rescued by compensatory charge neutralization of the scissile phosphate via methylphosphonate (MeP) modificati...

An Empirical Investigation of Architectural Heritage Management Implications for Tourism: The Case of Portugal

Directory of Open Access Journals (Sweden)

Shahrbanoo Gholitabar

2018-01-01

Full Text Available The aims of this study are manifold. First, to investigate the potentials of architectural heritage in the context of tourism destination development, as well as examine public sector policies and make plans toward the preservation of these resources. Secondly, to appraise the outcome of preservation and its implications for tourism. The study is an effort to explore and understand the interrelationships between tourism and architectural heritage sites through tourist image and perception. For the purposes of this research, numerous heritage sites were sampled in Portugal. A mixed research method was utilized to gauge tourists’ image/perception of heritage resources, and impact (quantitative approach. A qualitative approach was utilized to assess the priority of tourists in their visits and public-sector policies toward heritage resource management and planning. The fuzzy logic method was used to assess the architectural value and the tourist and preservation potential of historical buildings in Porto/Aveiro. The contribution and implications of the study are also explained. The results revealed that architectural heritage resources have the most appeal to tourists. The study to date demonstrates the architectural value and tourist and preservation potential of the buildings observed via evaluation by fuzzy logic methods.

Environment and Architecture - a Paradigm Shift

Science.gov (United States)

di Battista, Valerio

The interaction of human cultures and the built environment allows a wide range of interpretations and has been studied inside the domain of many disciplines. This paper discusses three interpretations descending from a systemic approach to the question: - architecture as an "emergence" of the settlement system; - place (and space) as an "accumulator" of time and a "flux" of systems; - landscape as one representation/description of the human settlement. Architecture emerges as a new physical conformation or layout, or as a change in a specific site, arising from actions and representations of political, religious, economical or social powers, being shaped at all times by the material culture belonging to a specific time and place in the course of human evolution. Any inhabited space becomes over time a place as well as a landscape, i.e. a representation of the settlement and a relationship between setting and people. Therefore, any place owns a landscape which, in turn, is a system of physical systems; it could be defined as a system of sites that builds up its own structure stemming from the orographical features and the geometry of land surfaces that set out the basic characters of its space.

European landscape architecture and territorial strategies for water landscapes

DEFF Research Database (Denmark)

Diedrich, Lisa Babette

2010-01-01

This article sums up the author’s lecture at the 2009 Sydney Resilient Water Landscapes Symposium and presents a series of realized or planned European landscape architectural and urbanistic projects on water landscapes taken from the recently published book On Site/ Landscape Architecture Europe...... and accompanying reflections. The hypothesis is that further scientific research can help defining weaknesses and strengths of the existing water landscape designs in terms of resilience, extract principles and tools, improve the weak ones and communicate the strong ones and develop general quality criteria...... and tools for future resilient water landscapes....

Eco-architecture: Nostalgia or present-day challenge

Energy Technology Data Exchange (ETDEWEB)

Spath, R.

1982-05-01

Ecological architecture contains the so-called alternative ways of building, taking into consideration passive energy systems in accordance with building-biological aspects and their natural incorporation in the landscape. The objective of ecological architecture is to connect the historical experiences in the adaptation of buildings to regional, local, and climate conditions with todays possibilities of building. This requests a changed planning of the building. Alternative architecture begins with the planning process and the user has to participate actively. It also includes the greening of the plot and the external arrangement.

Enterprise architecture evaluation using architecture framework and UML stereotypes

Directory of Open Access Journals (Sweden)

Narges Shahi

2014-08-01

Full Text Available There is an increasing need for enterprise architecture in numerous organizations with complicated systems with various processes. Support for information technology, organizational units whose elements maintain complex relationships increases. Enterprise architecture is so effective that its non-use in organizations is regarded as their institutional inability in efficient information technology management. The enterprise architecture process generally consists of three phases including strategic programing of information technology, enterprise architecture programing and enterprise architecture implementation. Each phase must be implemented sequentially and one single flaw in each phase may result in a flaw in the whole architecture and, consequently, in extra costs and time. If a model is mapped for the issue and then it is evaluated before enterprise architecture implementation in the second phase, the possible flaws in implementation process are prevented. In this study, the processes of enterprise architecture are illustrated through UML diagrams, and the architecture is evaluated in programming phase through transforming the UML diagrams to Petri nets. The results indicate that the high costs of the implementation phase will be reduced.

Active sites in char gasification: Final technical report

Energy Technology Data Exchange (ETDEWEB)

Wojtowicz, M.; Lilly, W.D.; Perkins, M.T.; Hradil, G.; Calo, J.M.; Suuberg, E.M.

1987-09-01

Among the key variables in the design of gasifiers and combustors is the reactivity of the chars which must be gasified or combusted. Significant loss of unburned char is unacceptable in virtually any process; the provision of sufficient residence time for complete conversion is essential. A very wide range of reactivities are observed, depending upon the nature of the char in a process. The current work focuses on furthering the understanding of gasification reactivities of chars. It has been well established that the reactivity of char to gasification generally depends upon three principal factors: (1) the concentration of ''active sites'' in the char; (2) mass transfer within the char; and (3) the type and concentration of catalytic impurities in the char. The present study primarily addresses the first factor. The subject of this research is the origin, nature, and fate of active sites in chars derived from parent hydrocarbons with coal-like structure. The nature and number of the active sites and their reactivity towards oxygen are examined in ''model'' chars derived from phenol-formaldehyde type resins. How the active sites are lost by the process of thermal annealing during heat treatment of chars are studied, and actual rate for the annealing process is derived. Since intrinsic char reactivities are of primary interest in the present study, a fair amount of attention was given to the model char synthesis and handling so that the effect of catalytic impurities and oxygen-containing functional groups in the chemical structure of the material were minimized, if not completely eliminated. The project would not be considered complete without comparing characteristic features of synthetic chars with kinetic behavior exhibited by natural chars, including coal chars.

Design Principles for E-Government Architectures

Science.gov (United States)

Sandoz, Alain

The paper introduces a holistic approach for architecting systems which must sustain the entire e-government activity of a public authority. Four principles directly impact the architecture: Legality, Responsibility, Transparency, and Symmetry leading to coherent representations of the architecture for the client, the designer and the builder. The approach enables to deploy multipartite, distributed public services, including legal delegation of roles and outsourcing of non mandatory tasks through PPP.

CIM overview. (2). ; Architecture, infrastructure, information technology. CIM soron. (2). ; Architecture, infra, joho riyo shien gijutsu

Energy Technology Data Exchange (ETDEWEB)

Seryo, K [IBM Japan Ltd., Tokyo (Japan)

1991-12-10

The materialization of computer integrated manufacturing (CIM) requires an establishment of its systematizing system, i.e., architecturer and planning of the infrastructure to support it and basic engineering to support the informational utilization. The CIM architecture is classified into management and system structure, strategic planning method, System development introduction method, etc. The infrastructure aims at epoch-makingly heightening the productivity and speed by integrating the production planning, engineering design, accounting, sales, general business and affairs, production engineering, production activities, and activities of suppliers and clients. The informational utilization support engineering comprises the management support tool, decision making support tool, application development tool, etc. What is important is to establish a system of systematizing engineering in order not to be behind the strategic activation era of information to come. 17 refs., 4 figs., 1 tab.

Software architecture 2

CERN Document Server

Oussalah, Mourad Chabanne

2014-01-01

Over the past 20 years, software architectures have significantly contributed to the development of complex and distributed systems. Nowadays, it is recognized that one of the critical problems in the design and development of any complex software system is its architecture, i.e. the organization of its architectural elements. Software Architecture presents the software architecture paradigms based on objects, components, services and models, as well as the various architectural techniques and methods, the analysis of architectural qualities, models of representation of architectural templa

Lightweight enterprise architectures

CERN Document Server

Theuerkorn, Fenix

2004-01-01

STATE OF ARCHITECTUREArchitectural ChaosRelation of Technology and Architecture The Many Faces of Architecture The Scope of Enterprise Architecture The Need for Enterprise ArchitectureThe History of Architecture The Current Environment Standardization Barriers The Need for Lightweight Architecture in the EnterpriseThe Cost of TechnologyThe Benefits of Enterprise Architecture The Domains of Architecture The Gap between Business and ITWhere Does LEA Fit? LEA's FrameworkFrameworks, Methodologies, and Approaches The Framework of LEATypes of Methodologies Types of ApproachesActual System Environmen

Software architecture 1

CERN Document Server

Oussalah , Mourad Chabane

2014-01-01

Over the past 20 years, software architectures have significantly contributed to the development of complex and distributed systems. Nowadays, it is recognized that one of the critical problems in the design and development of any complex software system is its architecture, i.e. the organization of its architectural elements. Software Architecture presents the software architecture paradigms based on objects, components, services and models, as well as the various architectural techniques and methods, the analysis of architectural qualities, models of representation of architectural template

Fostering Enterprise Architecture Education and Training with the Enterprise Architecture Competence Framework

Science.gov (United States)

Tambouris, Efthimios; Zotou, Maria; Kalampokis, Evangelos; Tarabanis, Konstantinos

2012-01-01

Enterprise architecture (EA) implementation refers to a set of activities ultimately aiming to align business objectives with information technology infrastructure in an organization. EA implementation is a multidisciplinary, complicated and endless process, hence, calls for adequate education and training programs that will build highly skilled…

Indigenous architecture as a context-oriented architecture, a look at ...

African Journals Online (AJOL)

What has become problematic as the achievement of international style and globalization of architecture during the time has been the purely technological look at architecture, and the architecture without belonging to a place. In recent decades, the topic of sustainable architecture and reconsidering indigenous architecture ...

The Complexity of Architecture : An Analysis of Design Intentions and Theories in the Norwegian National Tourist Routes

OpenAIRE

Roan, Beck

2013-01-01

This thesis analyzes the design intentions of architects in the Norwegian National Tourist Routes. Since architecture is not an isolated concept, this analysis incorporates philosophical and theoretical ideas in an investigation of the Tourist Route site design. Although they were hired to build compelling architectural sites along unique Norwegian nature, the Tourist Route architects also address subjects like practicality, balance, intention, meaning, control, freedom, and reflection in the...

Ligand uptake in Mycobacterium tuberculosis truncated hemoglobins is controlled by both internal tunnels and active site water molecules [version 2; referees: 2 approved

Directory of Open Access Journals (Sweden)

Ignacio Boron

2015-07-01

Full Text Available Mycobacterium tuberculosis, the causative agent of human tuberculosis, has two proteins belonging to the truncated hemoglobin (trHb family. Mt-trHbN presents well-defined internal hydrophobic tunnels that allow O2 and •ŸNO to migrate easily from the solvent to the active site, whereas Mt-trHbO possesses tunnels interrupted by a few bulky residues, particularly a tryptophan at position G8. Differential ligand migration rates allow Mt-trHbN to detoxify Ÿ•NO, a crucial step for pathogen survival once under attack by the immune system, much more efficiently than Mt-trHbO. In order to investigate the differences between these proteins, we performed experimental kinetic measurements, Ÿ•NO decomposition, as well as molecular dynamics simulations of the wild type Mt-trHbN and two mutants, VG8F and VG8W. These mutations affect both the tunnels accessibility as well as the affinity of distal site water molecules, thus modifying the ligand access to the iron. We found that a single mutation allows Mt-trHbN to acquire ligand migration rates comparable to those observed for Mt-trHbO, confirming that ligand migration is regulated by the internal tunnel architecture as well as by water molecules stabilized in the active site.

Living in Style: A Guide to American Architecture.

Science.gov (United States)

Instructor, 1990

1990-01-01

Studying architectural styles is an exciting way to make history come alive and to help students feel a part of their community. This teacher's guide includes several classroom activities, a historical guide to American architectural styles, a list of resources, and a reproducible student worksheet. (IAH)

Mapping the active site of vaccinia virus RNA triphosphatase

International Nuclear Information System (INIS)

Gong Chunling; Shuman, Stewart

2003-01-01

The RNA triphosphatase component of vaccinia virus mRNA capping enzyme (the product of the viral D1 gene) belongs to a family of metal-dependent phosphohydrolases that includes the RNA triphosphatases of fungi, protozoa, Chlorella virus, and baculoviruses. The family is defined by two glutamate-containing motifs (A and C) that form the metal-binding site. Most of the family members resemble the fungal and Chlorella virus enzymes, which have a complex active site located within the hydrophilic interior of a topologically closed eight-stranded β barrel (the so-called ''triphosphate tunnel''). Here we queried whether vaccinia virus capping enzyme is a member of the tunnel subfamily, via mutational mapping of amino acids required for vaccinia triphosphatase activity. We identified four new essential side chains in vaccinia D1 via alanine scanning and illuminated structure-activity relationships by conservative substitutions. Our results, together with previous mutational data, highlight a constellation of six acidic and three basic amino acids that likely compose the vaccinia triphosphatase active site (Glu37, Glu39, Arg77, Lys107, Glu126, Asp159, Lys161, Glu192, and Glu194). These nine essential residues are conserved in all vertebrate and invertebrate poxvirus RNA capping enzymes. We discerned no pattern of clustering of the catalytic residues of the poxvirus triphosphatase that would suggest structural similarity to the tunnel proteins (exclusive of motifs A and C). We infer that the poxvirus triphosphatases are a distinct lineage within the metal-dependent RNA triphosphatase family. Their unique active site, which is completely different from that of the host cell's capping enzyme, recommends the poxvirus RNA triphosphatase as a molecular target for antipoxviral drug discovery

Architecture in the Islamic Civilization: Muslim Building or Islamic Architecture

OpenAIRE

Yassin, Ayat Ali; Utaberta, Dr. Nangkula

2012-01-01

The main problem of the theory in the arena of islamic architecture is affected by some of its Westernthoughts, and stereotyping the islamic architecture according to Western thoughts; this leads to the breakdownof the foundations in the islamic architecture. It is a myth that islamic architecture is subjected to theinfluence from foreign architectures. This paper will highlight the dialectical concept of islamic architecture ormuslim buildings and the areas of recognition in islamic architec...

Enhancing User Customization through Novel Software Architecture for Utility Scale Solar Siting Software

Energy Technology Data Exchange (ETDEWEB)

Brant Peery; Sam Alessi; Randy Lee; Leng Vang; Scott Brown; David Solan; Dan Ames

2014-06-01

There is a need for a spatial decision support application that allows users to create customized metrics for comparing proposed locations of a new solar installation. This document discusses how PVMapper was designed to overcome the customization problem through the development of loosely coupled spatial and decision components in a JavaScript plugin architecture. This allows the user to easily add functionality and data to the system. The paper also explains how PVMapper provides the user with a dynamic and customizable decision tool that enables them to visually modify the formulas that are used in the decision algorithms that convert data to comparable metrics. The technologies that make up the presentation and calculation software stack are outlined. This document also explains the architecture that allows the tool to grow through custom plugins created by the software users. Some discussion is given on the difficulties encountered while designing the system.

Shared active site architecture between archaeal PolD and multi-subunit RNA polymerases revealed by X-ray crystallography.

Science.gov (United States)

Sauguet, Ludovic; Raia, Pierre; Henneke, Ghislaine; Delarue, Marc

2016-08-22

Archaeal replicative DNA polymerase D (PolD) constitute an atypical class of DNA polymerases made of a proofreading exonuclease subunit (DP1) and a larger polymerase catalytic subunit (DP2), both with unknown structures. We have determined the crystal structures of Pyrococcus abyssi DP1 and DP2 at 2.5 and 2.2 Å resolution, respectively, revealing a catalytic core strikingly different from all other known DNA polymerases (DNAPs). Rather, the PolD DP2 catalytic core has the same 'double-psi β-barrel' architecture seen in the RNA polymerase (RNAP) superfamily, which includes multi-subunit transcriptases of all domains of life, homodimeric RNA-silencing pathway RNAPs and atypical viral RNAPs. This finding bridges together, in non-viral world, DNA transcription and DNA replication within the same protein superfamily. This study documents further the complex evolutionary history of the DNA replication apparatus in different domains of life and proposes a classification of all extant DNAPs.

SABER: a computational method for identifying active sites for new reactions.

Science.gov (United States)

Nosrati, Geoffrey R; Houk, K N

2012-05-01

A software suite, SABER (Selection of Active/Binding sites for Enzyme Redesign), has been developed for the analysis of atomic geometries in protein structures, using a geometric hashing algorithm (Barker and Thornton, Bioinformatics 2003;19:1644-1649). SABER is used to explore the Protein Data Bank (PDB) to locate proteins with a specific 3D arrangement of catalytic groups to identify active sites that might be redesigned to catalyze new reactions. As a proof-of-principle test, SABER was used to identify enzymes that have the same catalytic group arrangement present in o-succinyl benzoate synthase (OSBS). Among the highest-scoring scaffolds identified by the SABER search for enzymes with the same catalytic group arrangement as OSBS were L-Ala D/L-Glu epimerase (AEE) and muconate lactonizing enzyme II (MLE), both of which have been redesigned to become effective OSBS catalysts, demonstrated by experiments. Next, we used SABER to search for naturally existing active sites in the PDB with catalytic groups similar to those present in the designed Kemp elimination enzyme KE07. From over 2000 geometric matches to the KE07 active site, SABER identified 23 matches that corresponded to residues from known active sites. The best of these matches, with a 0.28 Å catalytic atom RMSD to KE07, was then redesigned to be compatible with the Kemp elimination using RosettaDesign. We also used SABER to search for potential Kemp eliminases using a theozyme predicted to provide a greater rate acceleration than the active site of KE07, and used Rosetta to create a design based on the proteins identified. Copyright © 2012 The Protein Society.

GASS-WEB: a web server for identifying enzyme active sites based on genetic algorithms.

Science.gov (United States)

Moraes, João P A; Pappa, Gisele L; Pires, Douglas E V; Izidoro, Sandro C

2017-07-03

Enzyme active sites are important and conserved functional regions of proteins whose identification can be an invaluable step toward protein function prediction. Most of the existing methods for this task are based on active site similarity and present limitations including performing only exact matches on template residues, template size restraints, despite not being capable of finding inter-domain active sites. To fill this gap, we proposed GASS-WEB, a user-friendly web server that uses GASS (Genetic Active Site Search), a method based on an evolutionary algorithm to search for similar active sites in proteins. GASS-WEB can be used under two different scenarios: (i) given a protein of interest, to match a set of specific active site templates; or (ii) given an active site template, looking for it in a database of protein structures. The method has shown to be very effective on a range of experiments and was able to correctly identify >90% of the catalogued active sites from the Catalytic Site Atlas. It also managed to achieve a Matthew correlation coefficient of 0.63 using the Critical Assessment of protein Structure Prediction (CASP 10) dataset. In our analysis, GASS was ranking fourth among 18 methods. GASS-WEB is freely available at http://gass.unifei.edu.br/. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Report from the ATLAS Architecture TaskForce

CERN Document Server

Haywood, S

1999-01-01

In this report, the activities and conclusions of the ATLAS Architecture TaskForce (ATF) are summarised. A key part of the ATF's work has been the first attempt at a design of the global architecture for the ATLAS Offline Software. This is contained in this document and an auxillary report and should lead to a realisation of the ATLAS Framework.

Form follows function: the architecture of operational requirements. [Winstow Mine of UK

Energy Technology Data Exchange (ETDEWEB)

Crawford, C W

1984-07-01

Wistow Mine, in the Selby Coalfield (UK) has recently received a Festival of Architecture Award. The success of the visual appearance of the site is the result of careful consideration of the NCB's operational requirements, by a special design team, and the subsequent development of designs for all the buildings on the site and the layout of the site itself, which satisfy those requirements.

Report to the Board of Regents State University System of Florida. Review of Programs: Architecture, Architectural Technology, Landscape Architecture, Interior Design, Construction and Construction Technology, Building Construction, Urban and Regional Planning.

Science.gov (United States)

McMinn, William G.

An evaluation and report was done on the status of programs in architecture and related fields in the Florida State University System as a follow-up to a 1983 evaluation. The evaluation involved self-studies prepared by each program and a series of site visits to each of seven campuses and two centers with programs under review. These institutions…

Facebook, Twitter Activities Sites, Location and Students' Interest in Learning

Science.gov (United States)

Igbo, J. N.; Ezenwaji, Ifeyinwa; Ajuziogu, Christiana U.

2018-01-01

This study was carried out to ascertain the influence of social networking sites activities (twitter and Facebook) on secondary school students' interest in learning It also considered the impact of these social networking sites activities on location of the students. Two research questions and two null hypotheses guided the study. Mean and…

Using Carbohydrate Interaction Assays to Reveal Novel Binding Sites in Carbohydrate Active Enzymes

DEFF Research Database (Denmark)

Cockburn, Darrell; Wilkens, Casper; Dilokpimol, Adiphol

2016-01-01

Carbohydrate active enzymes often contain auxiliary binding sites located either on independent domains termed carbohydrate binding modules (CBMs) or as so-called surface binding sites (SBSs) on the catalytic module at a certain distance from the active site. The SBSs are usually critical...

Software architecture analysis tool : software architecture metrics collection

NARCIS (Netherlands)

Muskens, J.; Chaudron, M.R.V.; Westgeest, R.

2002-01-01

The Software Engineering discipline lacks the ability to evaluate software architectures. Here we describe a tool for software architecture analysis that is based on metrics. Metrics can be used to detect possible problems and bottlenecks in software architectures. Even though metrics do not give a

Poisoning Experiments Aimed at Discriminating Active and Less-Active Sites of Silica-Supported Tantalum Hydride for Alkane Metathesis

KAUST Repository

Saggio, Guillaume

2010-10-04

Only 50% of the silica-supported tantalum hydride sites are active in the metathesis of propane. Indeed, more than 45% of the tantalum hydride can be eliminated by a selective oxygen poisoning of inactive sites with no significant decrease in the global turnover. Conversely, cyclopentane induces no such selective poisoning. Hence, the active tantalum hydride sites that show greater resistance to oxygen poisoning correspond to the νTa-H bands of higher wavenumbers, particularly that at 1860cm-1. These active tantalum hydride sites should correspond to tris- or monohydride species relatively far from silica surface oxygen atoms. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The analysis of cultural architectural trends in Crisan locality

Directory of Open Access Journals (Sweden)

SELA Florentina

2010-09-01

Full Text Available The paper presents data about the identification and analyse of the traditional architectural elements in Crisan locality knowing that the tourism activity is in a continuous development. The field research (during November 2007 enabled us to develop a qualitative and quantitative analysis in terms of identification of traditional architecture elements, their conservation status, and frequency of traditional building materials use, decorative elements and specificcolors used in construction architecture. Further, based on collected data, was realized the chart - Distribution for TraditionalArchitecture Index (TAI on the distance from the center of Crisan locality, showing that in Crisan locality the houses were and are built without taking into account any rule, destroying thus traditional architecture.

Concept for calculating dose rates from activated groundwater at accelerator sites

CERN Document Server

Prolingheuer, N; Vanderborght, J; Schlögl, B; Nabbi, R; Moormann, R

Licensing of particle accelerators requires the proof that the groundwater outside of the site will not be significantly contaminated by activation products formed below accelerator and target. In order to reduce the effort for this proof, a site independent simplified but conservative method is under development. The conventional approach for calculation of activation of soil and groundwater is shortly described on example of a site close to Forschungszentrum Juelich, Germany. Additionally an updated overview of a data library for partition coefficients for relevant nuclides transported in the aquifer at the site is presented. The approximate model for transport of nuclides with ground water including exemplary results on nuclide concentrations outside of the site boundary and of resulting effective doses is described. Further applications and developments are finally outlined.

Neural codes of seeing architectural styles

OpenAIRE

Choo, Heeyoung; Nasar, Jack L.; Nikrahei, Bardia; Walther, Dirk B.

2017-01-01

Images of iconic buildings, such as the CN Tower, instantly transport us to specific places, such as Toronto. Despite the substantial impact of architectural design on people′s visual experience of built environments, we know little about its neural representation in the human brain. In the present study, we have found patterns of neural activity associated with specific architectural styles in several high-level visual brain regions, but not in primary visual cortex (V1). This finding sugges...

Open architecture design and approach for the Integrated Sensor Architecture (ISA)

Science.gov (United States)

Moulton, Christine L.; Krzywicki, Alan T.; Hepp, Jared J.; Harrell, John; Kogut, Michael

2015-05-01

Integrated Sensor Architecture (ISA) is designed in response to stovepiped integration approaches. The design, based on the principles of Service Oriented Architectures (SOA) and Open Architectures, addresses the problem of integration, and is not designed for specific sensors or systems. The use of SOA and Open Architecture approaches has led to a flexible, extensible architecture. Using these approaches, and supported with common data formats, open protocol specifications, and Department of Defense Architecture Framework (DoDAF) system architecture documents, an integration-focused architecture has been developed. ISA can help move the Department of Defense (DoD) from costly stovepipe solutions to a more cost-effective plug-and-play design to support interoperability.

Remediation of uranium contaminated sites: clean-up activities in Serbia

International Nuclear Information System (INIS)

Raicevic, S.; Raicevic, J. . E-mail address of corresponding author: raich@beotel.yu; Raicevic, S.)

2005-01-01

One of the serious environmental problems in Serbia represent sites contaminated with depleted uranium (DU) during past war activities. According to UNEP reports and our findings there are two types of contamination: (i) localized points of high, concentrated contamination where DU penetrators enter the soil, and (ii) low level of widespread DU contamination, which indicates that during the conflict DU dust was dispersed into the environment. Remediation of these sites is an urgent need because they represent a permanent threat to the population living in this area. Here we give a brief description of approaches commonly used in remediation of DU contaminated sites, and an overview of current clean-up activities performed in Serbia. (author)

Enterprise Architecture v systému řízení

OpenAIRE

Sládek, Pavel

2008-01-01

This thesis deals with subject of enterprise architecture management. Topcis covered are enterprise environment integration, enterprise architecture parts description and description of shift in enterprise architecture focus point. This trend can be described as shift from IT related activity for IT management through tool for IT and business alignment to the final state as a tool for business change implementation. There are four goals of this thesis. Description of enterprise architecture, ...

An Architectural Decision Tool Based on Scenarios and Non-functional Requirements

OpenAIRE

Mr. Mahesh Parmar; Prof. W.U. Khan; Dr. Binod Kumar

2011-01-01

Software architecture design is often based on architects intuition and previous experience. Little methodological support is available, but there are still no effective solutions to guide the architectural design. The most difficult activity is the transformation from non-functional requirement specification into software architecture. To achieve above things proposed “An Architectural Decision Tool Based on Scenarios and Nonfunctional Requirementsâ€. In this proposed tool scenarios are fi...

Embedded Real-Time Architecture for Level-Set-Based Active Contours

Directory of Open Access Journals (Sweden)

Dejnožková Eva

2005-01-01

Full Text Available Methods described by partial differential equations have gained a considerable interest because of undoubtful advantages such as an easy mathematical description of the underlying physics phenomena, subpixel precision, isotropy, or direct extension to higher dimensions. Though their implementation within the level set framework offers other interesting advantages, their vast industrial deployment on embedded systems is slowed down by their considerable computational effort. This paper exploits the high parallelization potential of the operators from the level set framework and proposes a scalable, asynchronous, multiprocessor platform suitable for system-on-chip solutions. We concentrate on obtaining real-time execution capabilities. The performance is evaluated on a continuous watershed and an object-tracking application based on a simple gradient-based attraction force driving the active countour. The proposed architecture can be realized on commercially available FPGAs. It is built around general-purpose processor cores, and can run code developed with usual tools.

Principles of landscape architecture

NARCIS (Netherlands)

Nijhuis, S.

2013-01-01

The Department of Urbanism at the Faculty of Architecture and Built Environment, TU Delft considers urbanism as a planning and design oriented activity towards urban and rural landscapes. It aims to enhance, restore or create landscapes from a perspective of sustainable development, so as to guide,

Analysis of Architecture Pattern Usage in Legacy System Architecture Documentation

NARCIS (Netherlands)

Harrison, Neil B.; Avgeriou, Paris

2008-01-01

Architecture patterns are an important tool in architectural design. However, while many architecture patterns have been identified, there is little in-depth understanding of their actual use in software architectures. For instance, there is no overview of how many patterns are used per system or

Structure of the human protein kinase MPSK1 reveals an atypical activation loop architecture.

Science.gov (United States)

Eswaran, Jeyanthy; Bernad, Antonio; Ligos, Jose M; Guinea, Barbara; Debreczeni, Judit E; Sobott, Frank; Parker, Sirlester A; Najmanovich, Rafael; Turk, Benjamin E; Knapp, Stefan

2008-01-01

The activation segment of protein kinases is structurally highly conserved and central to regulation of kinase activation. Here we report an atypical activation segment architecture in human MPSK1 comprising a beta sheet and a large alpha-helical insertion. Sequence comparisons suggested that similar activation segments exist in all members of the MPSK1 family and in MAST kinases. The consequence of this nonclassical activation segment on substrate recognition was studied using peptide library screens that revealed a preferred substrate sequence of X-X-P/V/I-phi-H/Y-T*-N/G-X-X-X (phi is an aliphatic residue). In addition, we identified the GTPase DRG1 as an MPSK1 interaction partner and specific substrate. The interaction domain in DRG1 was mapped to the N terminus, leading to recruitment and phosphorylation at Thr100 within the GTPase domain. The presented data reveal an atypical kinase structural motif and suggest a role of MPSK1 regulating DRG1, a GTPase involved in regulation of cellular growth.

On the Architectural Engineering Competences in Architectural Design

DEFF Research Database (Denmark)

Kirkegaard, Poul Henning

2007-01-01

In 1997 a new education in Architecture & Design at Department of Architecture and Design, Aalborg University was started with 50 students. During the recent years this number has increased to approximately 100 new students each year, i.e. approximately 500 students are following the 3 years...... bachelor (BSc) and the 2 years master (MSc) programme. The first 5 semesters are common for all students followed by 5 semesters with specialization into Architectural Design, Urban Design, Industrial Design or Digital Design. The present paper gives a short summary of the architectural engineering...

Functional characterization of autophosphorylation sites of the activated insulin receptor-tyrosine kinase

International Nuclear Information System (INIS)

Flores-Riveros, J.R.; Lane, M.D.

1987-01-01

Insulin receptor, solubilized from 3T3-L1 cellular membranes and then purified, was autophosphorylated with [γ- 32 P]ATP in the absence or presence of insulin. Specific phosphopeptides generated by trypsin digestion of the 32 P-labeled β-subunit were identified and separated by reverse phase HPLC. In the absence of insulin, radioactivity of the phosphopeptides is evenly distributed among four major peaks designated as sites I, II, III and IV, according to their order of elution. This pattern is maintained for at least the first 30 min of autophosphorylation. When the reaction is carried out in the presence of insulin, > 50% of the total 32 P radioactivity is found in site I and the rate of 32 P incorporation into this site is markedly higher than into sites II, III and IV. Maximal activation of tyrosine kinase activity, as estimated by substrate phosphorylation, is coincident with the nearly complete phosphorylation of site I. Delayed activation of previously autophosphorylated receptor by insulin, but not by EGF or IGF-I, produced a similar pattern where phosphorylated site I predominates. These observations indicate that one major insulin-regulated autophosphorylation site in the β-subunit is responsible for activation of the insulin receptor tyrosine kinase. The isolation of this phosphopeptide on a preparative scale and its characterization are now in progress

Assembling high activity phosphotriesterase composites using hybrid nanoparticle peptide-DNA scaffolded architectures

Science.gov (United States)

Breger, Joyce C.; Buckhout-White, Susan; Walper, Scott A.; Oh, Eunkeu; Susumu, Kimihiro; Ancona, Mario G.; Medintz, Igor L.

2017-06-01

Nanoparticle (NP) display potentially offers a new way to both stabilize and, in many cases, enhance enzyme activity over that seen for native protein in solution. However, the large, globular and sometimes multimeric nature of many enzymes limits their ability to attach directly to the surface of NPs, especially when the latter are colloidally stabilized with bulky PEGylated ligands. Engineering extended protein linkers into the enzymes to achieve direct attachment through the PEG surface often detrimentally alters the enzymes catalytic ability. Here, we demonstrate an alternate, hybrid biomaterials-based approach to achieving directed enzyme assembly on PEGylated NPs. We self-assemble a unique architecture consisting of a central semiconductor quantum dot (QD) scaffold displaying controlled ratios of extended peptide-DNA linkers which penetrate through the PEG surface to directly couple enzymes to the QD surface. As a test case, we utilize phosphotriesterase (PTE), an enzyme of bio-defense interest due to its ability to hydrolyze organophosphate nerve agents. Moreover, this unique approach still allows PTE to maintain enhanced activity while also suggesting the ability of DNA to enhance enzyme activity in and of itself.

Connecting Architecture and Implementation

Science.gov (United States)

Buchgeher, Georg; Weinreich, Rainer

Software architectures are still typically defined and described independently from implementation. To avoid architectural erosion and drift, architectural representation needs to be continuously updated and synchronized with system implementation. Existing approaches for architecture representation like informal architecture documentation, UML diagrams, and Architecture Description Languages (ADLs) provide only limited support for connecting architecture descriptions and implementations. Architecture management tools like Lattix, SonarJ, and Sotoarc and UML-tools tackle this problem by extracting architecture information directly from code. This approach works for low-level architectural abstractions like classes and interfaces in object-oriented systems but fails to support architectural abstractions not found in programming languages. In this paper we present an approach for linking and continuously synchronizing a formalized architecture representation to an implementation. The approach is a synthesis of functionality provided by code-centric architecture management and UML tools and higher-level architecture analysis approaches like ADLs.

Architecture of the bacteriophage T4 activator MotA/promoter DNA interaction during sigma appropriation.

Science.gov (United States)

Hsieh, Meng-Lun; James, Tamara D; Knipling, Leslie; Waddell, M Brett; White, Stephen; Hinton, Deborah M

2013-09-20

Gene expression can be regulated through factors that direct RNA polymerase to the correct promoter sequence at the correct time. Bacteriophage T4 controls its development in this way using phage proteins that interact with host RNA polymerase. Using a process called σ appropriation, the T4 co-activator AsiA structurally remodels the σ(70) subunit of host RNA polymerase, while a T4 activator, MotA, engages the C terminus of σ(70) and binds to a DNA promoter element, the MotA box. Structures for the N-terminal (NTD) and C-terminal (CTD) domains of MotA are available, but no structure exists for MotA with or without DNA. We report the first molecular map of the MotA/DNA interaction within the σ-appropriated complex, which we obtained by using the cleaving reagent, iron bromoacetamidobenzyl-EDTA (FeBABE). We conjugated surface-exposed, single cysteines in MotA with FeBABE and performed cleavage reactions in the context of stable transcription complexes. The DNA cleavage sites were analyzed using ICM Molsoft software and three-dimensional physical models of MotA(NTD), MotA(CTD), and the DNA to investigate shape complementarity between the protein and the DNA and to position MotA on the DNA. We found that the unusual "double wing" motif present within MotA(CTD) resides in the major groove of the MotA box. In addition, we have used surface plasmon resonance to show that MotA alone is in a very dynamic equilibrium with the MotA element. Our results demonstrate the utility of fine resolution FeBABE mapping to determine the architecture of protein-DNA complexes that have been recalcitrant to traditional structure analyses.

Active Sites Environmental Monitoring Program. FY 1993: Annual report

International Nuclear Information System (INIS)

Morrissey, C.M.; Ashwood, T.L.; Hicks, D.S.; Marsh, J.D.

1994-08-01

This report continues a series of annual and semiannual reports that present the results of the Active Sites Environmental Monitoring Program (ASEMP) monitoring activities. The report details monitoring data for fiscal year (FY) 1993 and is divided into three major areas: SWSA 6 [including tumulus pads, Interim Waste Management Facility (IWMF), and other sites], the low-level Liquid-Waste Solidification Project (LWSP), and TRU-waste storage facilities in SWSA 5 N. The detailed monitoring methodology is described in the second revision of the ASEMP program plan. This report also presents a summary of the methodology used to gather data for each major area along with the results obtained during FY 1993

Essential histidyl residues at the active site(s) of sucrose-phosphate synthase from Prosopis juliflora.

Science.gov (United States)

Sinha, A K; Pathre, U V; Sane, P V

1998-11-10

Chemical modification of sucrose-phosphate synthase (EC 2.4.1.14) from Prosopis juliflora by diethyl pyrocarbonate (DEP) and photo-oxidation in the presence of rose bengal (RB) which modify the histidyl residues of the protein resulted in the inactivation of the enzyme activity. This inactivation was dependent on the concentration of the modifying reagent and the time of incubation and followed pseudo-first order kinetics. For both the reagents, the inactivation was maximum at pH 7.5, which is consistent with the involvement and presence of histidine residues at the active site of the enzyme. Substrates, UDPG and F6P protected the enzyme against the inactivation by the modifying reagents suggesting that the histidine residues may be involved in the binding of these substrates and are essential for the catalytic activity. Specificity of DEP was indicated by an increase in absorbance at 240 nm along with concomitant inactivation of the enzyme and reactivation of the modified enzyme by hydroxylamine. These results strongly suggest the presence of histidine residue(s) at or near the active site of the enzyme.

Gideaa study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Nordqvist, R.; Ljunggren, C.; Tiren, S.; Voss, C.

1991-10-01

During the period from 1977-1986 SKB (Swedish Nuclear Fuel and Waste Management Co) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the study sites will be selected as a site for detailed characterization. Other site with geological and/or socio-economical characteristics judged more favourable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concerns the Gideaa study site. (au)

Kamlunge study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Andersson, P.; Ittner, T.; Tiren, S.; Ljunggren, C.

1992-05-01

During the period from 1977-1986 SKB (Swedish nuclear Fuel and Waste Management Co.) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favourable may very well be selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concerns the Kamlunge study site. (79 refs.) (au)

Fjaellveden study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Nordqvist, R.; Ljunggren, C.; Tiren, S.; Voss, C.

1991-10-01

During the period from 1977-1986 SKB (Swedish Nuclear Fuel and Waste Management CO) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favourable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need for complementary investigations. This report concerns the Fjaellveden study site. (au)

On the beginnings of cinema historiography: the architecture of a modern commercial activity

Directory of Open Access Journals (Sweden)

Andrés Ávila Gómez

2015-12-01

Full Text Available This article outlines a structured critical reflection of Ben Hall´s analysis in his The Best Remaining Seats. The Golden Age of the Movie Palace. When it was published in 1961, this article progressively provoked interest in commercial architecture. Its mutations foretold its own disappearance, in the ways that were known at that time: it was architecture for cinematographic exhibition. Beginning with the identification of the main historic elements that are suggested by Hall, this analysis seeks to establish some of the continuities or lack of continuities that they subsequently have in renowned researchers’ written works on the ever-changing cinema architecture.

How organisation of architecture documentation affects architectural knowledge retrieval

NARCIS (Netherlands)

de Graaf, K.A.; Liang, P.; Tang, A.; Vliet, J.C.

A common approach to software architecture documentation in industry projects is the use of file-based documents. This approach offers a single-dimensional arrangement of the architectural knowledge. Knowledge retrieval from file-based architecture documentation is efficient if the organisation of

Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites

Science.gov (United States)

Brautigam, Chad A; Xing, Wenmin; Yang, Sheng; Henry, Lisa; Doolittle, Lynda K; Walz, Thomas

2017-01-01

The Rho GTPase Rac1 activates the WAVE regulatory complex (WRC) to drive Arp2/3 complex-mediated actin polymerization, which underpins diverse cellular processes. Here we report the structure of a WRC-Rac1 complex determined by cryo-electron microscopy. Surprisingly, Rac1 is not located at the binding site on the Sra1 subunit of the WRC previously identified by mutagenesis and biochemical data. Rather, it binds to a distinct, conserved site on the opposite end of Sra1. Biophysical and biochemical data on WRC mutants confirm that Rac1 binds to both sites, with the newly identified site having higher affinity and both sites required for WRC activation. Our data reveal that the WRC is activated by simultaneous engagement of two Rac1 molecules, suggesting a mechanism by which cells may sense the density of active Rac1 at membranes to precisely control actin assembly. PMID:28949297

Nuclear waste: Status of DOE's nuclear waste site characterization activities

International Nuclear Information System (INIS)

1987-01-01

Three potential nuclear waste repository sites have been selected to carry out characterization activities-the detailed geological testing to determine the suitability of each site as a repository. The sites are Hanford in south-central Washington State, Yucca Mountain in southern Nevada, and Deaf Smith in the Texas Panhandle. Two key issues affecting the total program are the estimations of the site characterization completion data and costs and DOE's relationship with the Nuclear Regulatory Commission which has been limited and its relations with affected states and Indian tribes which continue to be difficult

Preserving urban objects of historicaland architectural heritage

Directory of Open Access Journals (Sweden)

Bal'zannikova Ekaterina Mikhailovna

2014-01-01

structural elements, delivering building materials, preparing the construction site and the basic period when condemned structures are demolished, new design elements are formed and assembled, interior finishing work is performed and the object facade is restored. In contrast to it, our method includes additional periods and a performance list. In particular, it is proposed to carry out a research period prior to the preparatory period, and after the basic period there should be the ending period.Thus, during the research period it is necessary to study urban development features in architectural and town-planning environment, to identify the historical and architectural value of the object, to estimate its ramshackle state and whether it is habitable, to determine the relationship of the object with the architectural and aesthetic image of surrounding objects and to develop a conservation program; and during the ending period it is proposed to assess the historical and architectural significance of the reconstructed object in relation to the aesthetic and architectural image of the surrounding area. The proposed complex method will increase the attractiveness of a historical and architectural heritage object and its surrounding area for tourists and, consequently, raise the cultural level of the visitors. Furthermore, the method will ensure the construction of recreation zones, their more frequent usage and visiting surrounding objects of social infrastructure, because more opportunities for cultural and aesthetic pastime will be offered. The method will also provide a more reasonable and effective use of available funding due to the careful analysis and proper choice of the methods to preserve objects of historical and architectural heritage.

Lab architecture

Science.gov (United States)

Crease, Robert P.

2008-04-01

There are few more dramatic illustrations of the vicissitudes of laboratory architecturethan the contrast between Building 20 at the Massachusetts Institute of Technology (MIT) and its replacement, the Ray and Maria Stata Center. Building 20 was built hurriedly in 1943 as temporary housing for MIT's famous Rad Lab, the site of wartime radar research, and it remained a productive laboratory space for over half a century. A decade ago it was demolished to make way for the Stata Center, an architecturally striking building designed by Frank Gehry to house MIT's computer science and artificial intelligence labs (above). But in 2004 - just two years after the Stata Center officially opened - the building was criticized for being unsuitable for research and became the subject of still ongoing lawsuits alleging design and construction failures.

Monitoring WLCG with lambda-architecture: a new scalable data store and analytics platform for monitoring at petabyte scale

International Nuclear Information System (INIS)

Magnoni, L; Cordeiro, C; Georgiou, M; Andreeva, J; Suthakar, U; Khan, A; Smith, D R

2015-01-01

Monitoring the WLCG infrastructure requires the gathering and analysis of a high volume of heterogeneous data (e.g. data transfers, job monitoring, site tests) coming from different services and experiment-specific frameworks to provide a uniform and flexible interface for scientists and sites. The current architecture, where relational database systems are used to store, to process and to serve monitoring data, has limitations in coping with the foreseen increase in the volume (e.g. higher LHC luminosity) and the variety (e.g. new data-transfer protocols and new resource-types, as cloud-computing) of WLCG monitoring events. This paper presents a new scalable data store and analytics platform designed by the Support for Distributed Computing (SDC) group, at the CERN IT department, which uses a variety of technologies each one targeting specific aspects of big-scale distributed data-processing (commonly referred as lambda-architecture approach). Results of data processing on Hadoop for WLCG data activities monitoring are presented, showing how the new architecture can easily analyze hundreds of millions of transfer logs in a few minutes. Moreover, a comparison of data partitioning, compression and file format (e.g. CSV, Avro) is presented, with particular attention given to how the file structure impacts the overall MapReduce performance. In conclusion, the evolution of the current implementation, which focuses on data storage and batch processing, towards a complete lambda-architecture is discussed, with consideration of candidate technology for the serving layer (e.g. Elasticsearch) and a description of a proof of concept implementation, based on Apache Spark and Esper, for the real-time part which compensates for batch-processing latency and automates problem detection and failures. (paper)

Monitoring WLCG with lambda-architecture: a new scalable data store and analytics platform for monitoring at petabyte scale.

Science.gov (United States)

Magnoni, L.; Suthakar, U.; Cordeiro, C.; Georgiou, M.; Andreeva, J.; Khan, A.; Smith, D. R.

2015-12-01

Monitoring the WLCG infrastructure requires the gathering and analysis of a high volume of heterogeneous data (e.g. data transfers, job monitoring, site tests) coming from different services and experiment-specific frameworks to provide a uniform and flexible interface for scientists and sites. The current architecture, where relational database systems are used to store, to process and to serve monitoring data, has limitations in coping with the foreseen increase in the volume (e.g. higher LHC luminosity) and the variety (e.g. new data-transfer protocols and new resource-types, as cloud-computing) of WLCG monitoring events. This paper presents a new scalable data store and analytics platform designed by the Support for Distributed Computing (SDC) group, at the CERN IT department, which uses a variety of technologies each one targeting specific aspects of big-scale distributed data-processing (commonly referred as lambda-architecture approach). Results of data processing on Hadoop for WLCG data activities monitoring are presented, showing how the new architecture can easily analyze hundreds of millions of transfer logs in a few minutes. Moreover, a comparison of data partitioning, compression and file format (e.g. CSV, Avro) is presented, with particular attention given to how the file structure impacts the overall MapReduce performance. In conclusion, the evolution of the current implementation, which focuses on data storage and batch processing, towards a complete lambda-architecture is discussed, with consideration of candidate technology for the serving layer (e.g. Elasticsearch) and a description of a proof of concept implementation, based on Apache Spark and Esper, for the real-time part which compensates for batch-processing latency and automates problem detection and failures.

weHelp: A Reference Architecture for Social Recommender Systems.

Science.gov (United States)

Sheth, Swapneel; Arora, Nipun; Murphy, Christian; Kaiser, Gail

2010-01-01

Recommender systems have become increasingly popular. Most of the research on recommender systems has focused on recommendation algorithms. There has been relatively little research, however, in the area of generalized system architectures for recommendation systems. In this paper, we introduce weHelp : a reference architecture for social recommender systems - systems where recommendations are derived automatically from the aggregate of logged activities conducted by the system's users. Our architecture is designed to be application and domain agnostic. We feel that a good reference architecture will make designing a recommendation system easier; in particular, weHelp aims to provide a practical design template to help developers design their own well-modularized systems.

Capital Architecture: Situating symbolism parallel to architectural methods and technology

Science.gov (United States)

Daoud, Bassam

Capital Architecture is a symbol of a nation's global presence and the cultural and social focal point of its inhabitants. Since the advent of High-Modernism in Western cities, and subsequently decolonised capitals, civic architecture no longer seems to be strictly grounded in the philosophy that national buildings shape the legacy of government and the way a nation is regarded through its built environment. Amidst an exceedingly globalized architectural practice and with the growing concern of key heritage foundations over the shortcomings of international modernism in representing its immediate socio-cultural context, the contextualization of public architecture within its sociological, cultural and economic framework in capital cities became the key denominator of this thesis. Civic architecture in capital cities is essential to confront the challenges of symbolizing a nation and demonstrating the legitimacy of the government'. In today's dominantly secular Western societies, governmental architecture, especially where the seat of political power lies, is the ultimate form of architectural expression in conveying a sense of identity and underlining a nation's status. Departing with these convictions, this thesis investigates the embodied symbolic power, the representative capacity, and the inherent permanence in contemporary architecture, and in its modes of production. Through a vast study on Modern architectural ideals and heritage -- in parallel to methodologies -- the thesis stimulates the future of large scale governmental building practices and aims to identify and index the key constituents that may respond to the lack representation in civic architecture in capital cities.

Software architecture evolution

DEFF Research Database (Denmark)

Barais, Olivier; Le Meur, Anne-Francoise; Duchien, Laurence

2008-01-01

Software architectures must frequently evolve to cope with changing requirements, and this evolution often implies integrating new concerns. Unfortunately, when the new concerns are crosscutting, existing architecture description languages provide little or no support for this kind of evolution....... The software architect must modify multiple elements of the architecture manually, which risks introducing inconsistencies. This chapter provides an overview, comparison and detailed treatment of the various state-of-the-art approaches to describing and evolving software architectures. Furthermore, we discuss...... one particular framework named Tran SAT, which addresses the above problems of software architecture evolution. Tran SAT provides a new element in the software architecture descriptions language, called an architectural aspect, for describing new concerns and their integration into an existing...

Knowledge Framework Implementation with Multiple Architectures - 13090

Energy Technology Data Exchange (ETDEWEB)

Upadhyay, H.; Lagos, L.; Quintero, W.; Shoffner, P. [Applied Research Center, Florida International University, Miami, FL 33174 (United States); DeGregory, J. [Office of D and D and Facility Engineering, Environmental Management, Department of Energy (United States)

2013-07-01

Multiple kinds of knowledge management systems are operational in public and private enterprises, large and small organizations with a variety of business models that make the design, implementation and operation of integrated knowledge systems very difficult. In recent days, there has been a sweeping advancement in the information technology area, leading to the development of sophisticated frameworks and architectures. These platforms need to be used for the development of integrated knowledge management systems which provides a common platform for sharing knowledge across the enterprise, thereby reducing the operational inefficiencies and delivering cost savings. This paper discusses the knowledge framework and architecture that can be used for the system development and its application to real life need of nuclear industry. A case study of deactivation and decommissioning (D and D) is discussed with the Knowledge Management Information Tool platform and framework. D and D work is a high priority activity across the Department of Energy (DOE) complex. Subject matter specialists (SMS) associated with DOE sites, the Energy Facility Contractors Group (EFCOG) and the D and D community have gained extensive knowledge and experience over the years in the cleanup of the legacy waste from the Manhattan Project. To prevent the D and D knowledge and expertise from being lost over time from the evolving and aging workforce, DOE and the Applied Research Center (ARC) at Florida International University (FIU) proposed to capture and maintain this valuable information in a universally available and easily usable system. (authors)

Knowledge Framework Implementation with Multiple Architectures - 13090

International Nuclear Information System (INIS)

Upadhyay, H.; Lagos, L.; Quintero, W.; Shoffner, P.; DeGregory, J.

2013-01-01

Multiple kinds of knowledge management systems are operational in public and private enterprises, large and small organizations with a variety of business models that make the design, implementation and operation of integrated knowledge systems very difficult. In recent days, there has been a sweeping advancement in the information technology area, leading to the development of sophisticated frameworks and architectures. These platforms need to be used for the development of integrated knowledge management systems which provides a common platform for sharing knowledge across the enterprise, thereby reducing the operational inefficiencies and delivering cost savings. This paper discusses the knowledge framework and architecture that can be used for the system development and its application to real life need of nuclear industry. A case study of deactivation and decommissioning (D and D) is discussed with the Knowledge Management Information Tool platform and framework. D and D work is a high priority activity across the Department of Energy (DOE) complex. Subject matter specialists (SMS) associated with DOE sites, the Energy Facility Contractors Group (EFCOG) and the D and D community have gained extensive knowledge and experience over the years in the cleanup of the legacy waste from the Manhattan Project. To prevent the D and D knowledge and expertise from being lost over time from the evolving and aging workforce, DOE and the Applied Research Center (ARC) at Florida International University (FIU) proposed to capture and maintain this valuable information in a universally available and easily usable system. (authors)

What Motivates Young Adults to Talk About Physical Activity on Social Network Sites?

Science.gov (United States)

Zhang, Ni; Campo, Shelly; Yang, Jingzhen; Eckler, Petya; Snetselaar, Linda; Janz, Kathleen; Leary, Emily

2017-06-22

Electronic word-of-mouth on social network sites has been used successfully in marketing. In social marketing, electronic word-of-mouth about products as health behaviors has the potential to be more effective and reach more young adults than health education through traditional mass media. However, little is known about what motivates people to actively initiate electronic word-of-mouth about health behaviors on their personal pages or profiles on social network sites, thus potentially reaching all their contacts on those sites. This study filled the gap by applying a marketing theoretical model to explore the factors associated with electronic word-of-mouth on social network sites about leisure-time physical activity. A Web survey link was sent to undergraduate students at one of the Midwestern universities and 439 of them completed the survey. The average age of the 439 participants was 19 years (SD=1 year, range: 18-24). Results suggested that emotional engagement with leisure-time physical activity (ie, affective involvement in leisure-time physical activity) predicted providing relevant opinions or information on social network sites. Social network site users who perceived stronger ties with all their contacts were more likely to provide and seek leisure-time physical activity opinions and information. People who provided leisure-time physical activity opinions and information were more likely to seek opinions and information, and people who forwarded information about leisure-time physical activity were more likely to chat about it. This study shed light on the application of the electronic word-of-mouth theoretical framework in promoting health behaviors. The findings can also guide the development of future social marketing interventions using social network sites to promote leisure-time physical activity. ©Ni Zhang, Shelly Campo, Jingzhen Yang, Petya Eckler, Linda Snetselaar, Kathleen Janz, Emily Leary. Originally published in the Journal of Medical

Architectures for a quantum random access memory

Science.gov (United States)

Giovannetti, Vittorio; Lloyd, Seth; Maccone, Lorenzo

2008-11-01

A random access memory, or RAM, is a device that, when interrogated, returns the content of a memory location in a memory array. A quantum RAM, or qRAM, allows one to access superpositions of memory sites, which may contain either quantum or classical information. RAMs and qRAMs with n -bit addresses can access 2n memory sites. Any design for a RAM or qRAM then requires O(2n) two-bit logic gates. At first sight this requirement might seem to make large scale quantum versions of such devices impractical, due to the difficulty of constructing and operating coherent devices with large numbers of quantum logic gates. Here we analyze two different RAM architectures (the conventional fanout and the “bucket brigade”) and propose some proof-of-principle implementations, which show that, in principle, only O(n) two-qubit physical interactions need take place during each qRAM call. That is, although a qRAM needs O(2n) quantum logic gates, only O(n) need to be activated during a memory call. The resulting decrease in resources could give rise to the construction of large qRAMs that could operate without the need for extensive quantum error correction.

Architectural design decisions

NARCIS (Netherlands)

Jansen, Antonius Gradus Johannes

2008-01-01

A software architecture can be considered as the collection of key decisions concerning the design of the software of a system. Knowledge about this design, i.e. architectural knowledge, is key for understanding a software architecture and thus the software itself. Architectural knowledge is mostly

The plasma automata network (PAN) architecture

International Nuclear Information System (INIS)

Cameron-Carey, C.M.

1991-01-01

Conventional neural networks consist of processing elements which are interconnected according to a specified topology. Typically, the number of processing elements and the interconnection topology are fixed. A neural network's information processing capability lies mainly in the variability of interconnection strengths, which directly influence activation patterns; these patterns represent entities and their interrelationships. Contrast this architecture, with its fixed topology and variable interconnection strengths, against one having dynamic topology and fixed connection strength. This paper reports on this proposed architecture in which there are no connections between processing elements. Instead, the processing elements form a plasma, exchanging information upon collision. A plasma can be populated with several different types of processing elements, each with their won activation function and self-modification mechanism. The activation patterns that are the plasma;s response to stimulation drive natural selection among processing elements which evolve to optimize performance

Architecture Governance: The Importance of Architecture Governance for Achieving Operationally Responsive Ground Systems

Science.gov (United States)

Kolar, Mike; Estefan, Jeff; Giovannoni, Brian; Barkley, Erik

2011-01-01

Topics covered (1) Why Governance and Why Now? (2) Characteristics of Architecture Governance (3) Strategic Elements (3a) Architectural Principles (3b) Architecture Board (3c) Architecture Compliance (4) Architecture Governance Infusion Process. Governance is concerned with decision making (i.e., setting directions, establishing standards and principles, and prioritizing investments). Architecture governance is the practice and orientation by which enterprise architectures and other architectures are managed and controlled at an enterprise-wide level

Molecular architecture of protein-RNA recognition sites.

Science.gov (United States)

Barik, Amita; C, Nithin; Pilla, Smita P; Bahadur, Ranjit Prasad

2015-01-01

The molecular architecture of protein-RNA interfaces are analyzed using a non-redundant dataset of 152 protein-RNA complexes. We find that an average protein-RNA interface is smaller than an average protein-DNA interface but larger than an average protein-protein interface. Among the different classes of protein-RNA complexes, interfaces with tRNA are the largest, while the interfaces with the single-stranded RNA are the smallest. Significantly, RNA contributes more to the interface area than its partner protein. Moreover, unlike protein-protein interfaces where the side chain contributes less to the interface area compared to the main chain, the main chain and side chain contributions flipped in protein-RNA interfaces. We find that the protein surface in contact with the RNA in protein-RNA complexes is better packed than that in contact with the DNA in protein-DNA complexes, but loosely packed than that in contact with the protein in protein-protein complexes. Shape complementarity and electrostatic potential are the two major factors that determine the specificity of the protein-RNA interaction. We find that the H-bond density at the protein-RNA interfaces is similar with that of protein-DNA interfaces but higher than the protein-protein interfaces. Unlike protein-DNA interfaces where the deoxyribose has little role in intermolecular H-bonds, due to the presence of an oxygen atom at the 2' position, the ribose in RNA plays significant role in protein-RNA H-bonds. We find that besides H-bonds, salt bridges and stacking interactions also play significant role in stabilizing protein-nucleic acids interfaces; however, their contribution at the protein-protein interfaces is insignificant.

Minimalism in architecture: Architecture as a language of its identity

Directory of Open Access Journals (Sweden)

Vasilski Dragana

2012-01-01

Full Text Available Every architectural work is created on the principle that includes the meaning, and then this work is read like an artifact of the particular meaning. Resources by which the meaning is built primarily, susceptible to transformation, as well as routing of understanding (decoding messages carried by a work of architecture, are subject of semiotics and communication theories, which have played significant role for the architecture and the architect. Minimalism in architecture, as a paradigm of the XXI century architecture, means searching for essence located in the irreducible minimum. Inspired use of architectural units (archetypical elements, trough the fatasm of simplicity, assumes the primary responsibility for providing the object identity, because it participates in language formation and therefore in its reading. Volume is form by clean language that builds the expression of the fluid areas liberated of recharge needs. Reduced architectural language is appropriating to the age marked by electronic communications.

Seismic risk assessment of architectural heritages in Gyeongju considering local site effects

Science.gov (United States)

Park, H.-J.; Kim, D.-S.; Kim, D.-M.

2013-02-01

A seismic risk assessment is conducted for cultural heritage sites in Gyeongju, the capital of Korea's ancient Silla Kingdom. Gyeongju, home to UNESCO World Heritage sites, contains remarkable artifacts of Korean Buddhist art. An extensive geotechnical survey including a series of in situ tests is presented, providing pertinent soil profiles for site response analyses on thirty cultural heritage sites. After the shear wave velocity profiles and dynamic material properties were obtained, site response analyses were carried out at each historical site and the amplification characteristics, site period, and response spectrum of the site were determined for the earthquake levels of 2400 yr and 1000 yr return periods based on the Korean seismic hazard map. Response spectrum and corresponding site coefficients obtained from site response analyses considering geologic conditions differ significantly from the current Korean seismic code. This study confirms the importance of site-specific ground response analyses considering local geological conditions. Results are given in the form of the spatial distribution of bedrock depth, site period, and site amplification coefficients, which are particularly valuable in the context of a seismic vulnerability study. This study presents the potential amplification of hazard maps and provides primary data on the seismic risk assessment of each cultural heritage.

Cooperative activation of cardiac transcription through myocardin bridging of paired MEF2 sites

Energy Technology Data Exchange (ETDEWEB)

Anderson, Courtney M. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Hu, Jianxin [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Thomas, Reuben [Univ. of California, San Francisco, CA (United States). Gladstone Inst.; Gainous, T. Blair [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Celona, Barbara [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Sinha, Tanvi [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Dickel, Diane E. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Genomics Division; Heidt, Analeah B. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Xu, Shan-Mei [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Bruneau, Benoit G. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Univ. of California, San Francisco, CA (United States). Gladstone Inst.; Pollard, Katherine S. [Univ. of California, San Francisco, CA (United States). Gladstone Inst.; Pennacchio, Len A. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Genomics Division; Black, Brian L. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Univ. of California, San Francisco, CA (United States). Dept. of

2017-03-28

Enhancers frequently contain multiple binding sites for the same transcription factor. These homotypic binding sites often exhibit synergy, whereby the transcriptional output from two or more binding sites is greater than the sum of the contributions of the individual binding sites alone. Although this phenomenon is frequently observed, the mechanistic basis for homotypic binding site synergy is poorly understood. Here in this paper, we identify a bona fide cardiac-specific Prkaa2 enhancer that is synergistically activated by homotypic MEF2 binding sites. We show that two MEF2 sites in the enhancer function cooperatively due to bridging of the MEF2C-bound sites by the SAP domain-containing co-activator protein myocardin, and we show that paired sites buffer the enhancer from integration site-dependent effects on transcription in vivo. Paired MEF2 sites are prevalent in cardiac enhancers, suggesting that this might be a common mechanism underlying synergy in the control of cardiac gene expression in vivo.

'Modani' and the Italian Building Site 1300-1600

DEFF Research Database (Denmark)

Foote, Jonathan

2016-01-01

a subsequent set of material translations onto tin or wood in preparation for the actual construction site. Although they were ostensibly tools for construction, new research has shown that modani provided a site for architectural invention as well, acting as a key instrument for extending architectural...... authorship onto the building site. Following the widespread reliance on 1:1 scaled drawings to study ancient building profiles, the making of modani became a critical practice for imagining new profiles as well. Modani also acted as key drawings in the communication of design intent and authorship...... to building patrons. Offering new assessments, analyses of the terminology, and an updated bibliography, this paper offers the first comprehensive survey of modani and sagome in the Italian context. It adds new discoveries into how they were utilized by architects, their technological agency...

Architectural Narratives

DEFF Research Database (Denmark)

Kiib, Hans

2010-01-01

a functional framework for these concepts, but tries increasingly to endow the main idea of the cultural project with a spatially aesthetic expression - a shift towards “experience architecture.” A great number of these projects typically recycle and reinterpret narratives related to historical buildings......In this essay, I focus on the combination of programs and the architecture of cultural projects that have emerged within the last few years. These projects are characterized as “hybrid cultural projects,” because they intend to combine experience with entertainment, play, and learning. This essay...... and architectural heritage; another group tries to embed new performative technologies in expressive architectural representation. Finally, this essay provides a theoretical framework for the analysis of the political rationales of these projects and for the architectural representation bridges the gap between...

The Open Science Grid status and architecture

Energy Technology Data Exchange (ETDEWEB)

Pordes, Ruth; Petravick, Don; /Fermilab; Kramer, Bill; Olsen, James D.; /LBL, Berkeley; Livny, Miron; Roy, Gordon A.; /Wisconsin U., Madison; Avery, Paul Ralph; /Florida U.; Blackburn, Kent; /Caltech; Wenaus, Torre J.; /Brookhaven; Wuerthwein, Frank K.; /UC, San Diego; Foster, Ian; /Chicago U. /Indiana U.

2007-09-01

The Open Science Grid (OSG) provides a distributed facility where the Consortium members provide guaranteed and opportunistic access to shared computing and storage resources. The OSG project[1] is funded by the National Science Foundation and the Department of Energy Scientific Discovery through Advanced Computing program. The OSG project provides specific activities for the operation and evolution of the common infrastructure. The US ATLAS and US CMS collaborations contribute to and depend on OSG as the US infrastructure contributing to the World Wide LHC Computing Grid on which the LHC experiments distribute and analyze their data. Other stakeholders include the STAR RHIC experiment, the Laser Interferometer Gravitational-Wave Observatory (LIGO), the Dark Energy Survey (DES) and several Fermilab Tevatron experiments- CDF, D0, MiniBoone etc. The OSG implementation architecture brings a pragmatic approach to enabling vertically integrated community specific distributed systems over a common horizontal set of shared resources and services. More information can be found at the OSG web site: www.opensciencegrid.org.

The Open Science Grid status and architecture

International Nuclear Information System (INIS)

Pordes, R; Petravick, D; Kramer, B; Olson, D; Livny, M; Roy, A; Avery, P; Blackburn, K; Wenaus, T; Wuerthwein, F; Foster, I; Gardner, R; Wilde, M; Blatecky, A; McGee, J; Quick, R

2008-01-01

The Open Science Grid (OSG) provides a distributed facility where the Consortium members provide guaranteed and opportunistic access to shared computing and storage resources. The OSG project[1] is funded by the National Science Foundation and the Department of Energy Scientific Discovery through Advanced Computing program. The OSG project provides specific activities for the operation and evolution of the common infrastructure. The US ATLAS and US CMS collaborations contribute to and depend on OSG as the US infrastructure contributing to the World Wide LHC Computing Grid on which the LHC experiments distribute and analyze their data. Other stakeholders include the STAR RHIC experiment, the Laser Interferometer Gravitational-Wave Observatory (LIGO), the Dark Energy Survey (DES) and several Fermilab Tevatron experiments- CDF, D0, MiniBoone etc. The OSG implementation architecture brings a pragmatic approach to enabling vertically integrated community specific distributed systems over a common horizontal set of shared resources and services. More information can be found at the OSG web site: www.opensciencegrid.org

Molecular Architecture of the Yeast Monopolin Complex

Energy Technology Data Exchange (ETDEWEB)

Corbett, Kevin D.; Harrison, Stephen C. (Harvard-Med); (UCSD)

2012-07-30

The Saccharomyces cerevisiae monopolin complex directs proper chromosome segregation in meiosis I by mediating co-orientation of sister kinetochores on the meiosis I spindle. The monopolin subunits Csm1 and Lrs4 form a V-shaped complex that may directly crosslink sister kinetochores. We report here biochemical characterization of the monopolin complex subunits Mam1 and Hrr25 and of the complete four-protein monopolin complex. By purifying monopolin subcomplexes with different subunit combinations, we have determined the stoichiometry and overall architecture of the full monopolin complex. We have determined the crystal structure of Csm1 bound to a Mam1 fragment, showing how Mam1 wraps around the Csm1 dimer and alters the stoichiometry of kinetochore-protein binding by Csm1. We further show that the kinase activity of Hrr25 is altered by Mam1 binding, and we identify Hrr25 phosphorylation sites on Mam1 that may affect monopolin complex stability and/or kinetochore binding in meiosis.

Immobilized low-activity waste site borehole 299-E17-21

International Nuclear Information System (INIS)

Reidel, S.P.; Reynolds, K.D.; Horton, D.G.

1998-08-01

The Tank Waste Remediation System (TWRS) is the group at the Hanford Site responsible for the safe underground storage of liquid waste from previous Hanford Site operations, the storage and disposal of immobilized tank waste, and closure of underground tanks. The current plan is to dispose of immobilized low-activity tank waste (ILAW) in new facilities in the southcentral part of 200-East Area and in four existing vaults along the east side of 200-East Area. Boreholes 299-E17-21, B8501, and B8502 were drilled at the southwest corner of the ILAW site in support of the Performance Assessment activities for the disposal options. This report summarizes the initial geologic findings, field tests conducted on those boreholes, and ongoing studies. One deep (480 feet) borehole and two shallow (50 feet) boreholes were drilled at the southwest corner of the ILAW site. The primary factor dictating the location of the boreholes was their characterization function with respect to developing the geohydrologic model for the site and satisfying associated Data Quality Objectives. The deep borehole was drilled to characterize subsurface conditions beneath the ILAW site, and two shallow boreholes were drilled to support an ongoing environmental tracer study. The tracer study will supply information to the Performance Assessment. All the boreholes provide data on the vadose zone and saturated zone in a previously uncharacterized area

Architecture & Environment

Science.gov (United States)

Erickson, Mary; Delahunt, Michael

2010-01-01

Most art teachers would agree that architecture is an important form of visual art, but they do not always include it in their curriculums. In this article, the authors share core ideas from "Architecture and Environment," a teaching resource that they developed out of a long-term interest in teaching architecture and their fascination with the…

Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality.

Science.gov (United States)

Zhou, Quansheng; Kapoor, Mili; Guo, Min; Belani, Rajesh; Xu, Xiaoling; Kiosses, William B; Hanan, Melanie; Park, Chulho; Armour, Eva; Do, Minh-Ha; Nangle, Leslie A; Schimmel, Paul; Yang, Xiang-Lei

2010-01-01

Protein multifunctionality is an emerging explanation for the complexity of higher organisms. In this regard, aminoacyl tRNA synthetases catalyze amino acid activation for protein synthesis, but some also act in pathways for inflammation, angiogenesis and apoptosis. It is unclear how these multiple functions evolved and how they relate to the active site. Here structural modeling analysis, mutagenesis and cell-based functional studies show that the potent angiostatic, natural fragment of human tryptophanyl-tRNA synthetase (TrpRS) associates via tryptophan side chains that protrude from its cognate cellular receptor vascular endothelial cadherin (VE-cadherin). VE-cadherin's tryptophan side chains fit into the tryptophan-specific active site of the synthetase. Thus, specific side chains of the receptor mimic amino acid substrates and expand the functionality of the active site of the synthetase. We propose that orthogonal use of the same active site may be a general way to develop multifunctionality of human tRNA synthetases and other proteins.

Exporting Humanist Architecture

DEFF Research Database (Denmark)

Nielsen, Tom

2016-01-01

The article is a chapter in the catalogue for the Danish exhibition at the 2016 Architecture Biennale in Venice. The catalogue is conceived at an independent book exploring the theme Art of Many - The Right to Space. The chapter is an essay in this anthology tracing and discussing the different...... values and ethical stands involved in the export of Danish Architecture. Abstract: Danish architecture has, in a sense, been driven by an unwritten contract between the architects and the democratic state and its institutions. This contract may be viewed as an ethos – an architectural tradition...... with inherent aesthetic and moral values. Today, however, Danish architecture is also an export commodity. That raises questions, which should be debated as openly as possible. What does it mean for architecture and architects to practice in cultures and under political systems that do not use architecture...

In silico analysis of Pycnoporus cinnabarinus laccase active site with toxic industrial dyes.

Science.gov (United States)

Prasad, Nirmal K; Vindal, Vaibhav; Narayana, Siva Lakshmi; Ramakrishna, V; Kunal, Swaraj Priyaranjan; Srinivas, M

2012-05-01

Laccases belong to multicopper oxidases, a widespread class of enzymes implicated in many oxidative functions in various industrial oxidative processes like production of fine chemicals to bioremediation of contaminated soil and water. In order to understand the mechanisms of substrate binding and interaction between substrates and Pycnoporus cinnabarinus laccase, a homology model was generated. The resulted model was further validated and used for docking studies with toxic industrial dyes- acid blue 74, reactive black 5 and reactive blue 19. Interactions of chemical mediators with the laccase was also examined. The docking analysis showed that the active site always cannot accommodate the dye molecules, due to constricted nature of the active site pocket and steric hindrance of the residues whereas mediators are relatively small and can easily be accommodated into the active site pocket, which, thereafter leads to the productive binding. The binding properties of these compounds along with identification of critical active site residues can be used for further site-directed mutagenesis experiments in order to identify their role in activity and substrate specificity, ultimately leading to improved mutants for degradation of these toxic compounds.

Sternoe study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Nordqvist, R.; Tiren, S.; Ljunggren, C.; Voss, C.

1992-01-01

During the period from 1977-1986 SKB (Swedish Nuclear Fuel and Waste Management Co) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the previously investigated study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favourable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concerns the Sternoe study site. This site was one of the first sites to be investigated by SKB . The studies at Sternoe were made under severe time-constraints and with prototype borehole instrumentations. These limitations should be kept in mind when reading the report. (41 refs., 16 figs., 12 tabs.) (au)

Fragments of Architecture

DEFF Research Database (Denmark)

Bang, Jacob Sebastian

2016-01-01

Topic 3: “Case studies dealing with the artistic and architectural work of architects worldwide, and the ties between specific artistic and architectural projects, methodologies and products”......Topic 3: “Case studies dealing with the artistic and architectural work of architects worldwide, and the ties between specific artistic and architectural projects, methodologies and products”...

Fabrication of TiO{sub 2} hierarchical architecture assembled by nanowires with anatase/TiO{sub 2}(B) phase-junctions for efficient photocatalytic hydrogen production

Energy Technology Data Exchange (ETDEWEB)

Qiu, Yong; Ouyang, Feng, E-mail: ouyangfh@hit.edu.cn

2017-05-01

Highlights: • H-titanate nanowires hierarchical architectures (TNH) were prepared by a hydrothermal method. • Calcinations of TNH leads to the formation of anatase/TiO{sub 2}(B) phase-junctions. • The hierarchical architecture offered enhanced light harvesting and large specific surface area. • The 1D nanowires and anatase/TiO{sub 2}(B) phase-junctions both can enhance the separation of photoinduced electron-hole. • The products calcined at the optimum conditions (450 °C) exhibited a maximum hydrogenproduction rate of 7808 μmol g{sup −1} h{sup −1}. - Abstract: TiO{sub 2} hierarchical architecture assembled by nanowires with anatase/TiO{sub 2}(B) phase-junctions was prepared by a hydrothermal process followed by calcinations. The optimum calcination treatment (450 °C) not only led to the formation of anatase/TiO{sub 2}(B) phase-junctions, but also kept the morphology of 1D nanowire and hierarchical architecture well. The T-450 load 0.5 wt% Pt cocatalysts showed the best photocatalytic hydrogen production activity, with a maximum hydrogen production rate of 7808 μmol g{sup −1} h{sup −1}. The high photocatalytic activity is ascribed to the combined effects of the following three factors: (1) the hierarchical architecture exhibits better light harvesting; (2) the larger specific surface area provides more surface active sites for the photocatalytic reaction; (3) the 1D nanowires and anatase/TiO{sub 2}(B) phase-junctions both can enhance the separation of photoinduced electron-hole pairs and inhibit their recombination.

Enterprise architecture management

DEFF Research Database (Denmark)

Rahimi, Fatemeh; Gøtze, John; Møller, Charles

2017-01-01

Despite the growing interest in enterprise architecture management, researchers and practitioners lack a shared understanding of its applications in organizations. Building on findings from a literature review and eight case studies, we develop a taxonomy that categorizes applications of enterprise...... architecture management based on three classes of enterprise architecture scope. Organizations may adopt enterprise architecture management to help form, plan, and implement IT strategies; help plan and implement business strategies; or to further complement the business strategy-formation process....... The findings challenge the traditional IT-centric view of enterprise architecture management application and suggest enterprise architecture management as an approach that could support the consistent design and evolution of an organization as a whole....

Enterprise architecture management

DEFF Research Database (Denmark)

Rahimi, Fatemeh; Gøtze, John; Møller, Charles

2017-01-01

architecture management based on three classes of enterprise architecture scope. Organizations may adopt enterprise architecture management to help form, plan, and implement IT strategies; help plan and implement business strategies; or to further complement the business strategy-formation process......Despite the growing interest in enterprise architecture management, researchers and practitioners lack a shared understanding of its applications in organizations. Building on findings from a literature review and eight case studies, we develop a taxonomy that categorizes applications of enterprise....... The findings challenge the traditional IT-centric view of enterprise architecture management application and suggest enterprise architecture management as an approach that could support the consistent design and evolution of an organization as a whole....

Thermodynamic compensation upon binding to exosite 1 and the active site of thrombin.

Science.gov (United States)

Treuheit, Nicholas A; Beach, Muneera A; Komives, Elizabeth A

2011-05-31

Several lines of experimental evidence including amide exchange and NMR suggest that ligands binding to thrombin cause reduced backbone dynamics. Binding of the covalent inhibitor dPhe-Pro-Arg chloromethyl ketone to the active site serine, as well as noncovalent binding of a fragment of the regulatory protein, thrombomodulin, to exosite 1 on the back side of the thrombin molecule both cause reduced dynamics. However, the reduced dynamics do not appear to be accompanied by significant conformational changes. In addition, binding of ligands to the active site does not change the affinity of thrombomodulin fragments binding to exosite 1; however, the thermodynamic coupling between exosite 1 and the active site has not been fully explored. We present isothermal titration calorimetry experiments that probe changes in enthalpy and entropy upon formation of binary ligand complexes. The approach relies on stringent thrombin preparation methods and on the use of dansyl-l-arginine-(3-methyl-1,5-pantanediyl)amide and a DNA aptamer as ligands with ideal thermodynamic signatures for binding to the active site and to exosite 1. Using this approach, the binding thermodynamic signatures of each ligand alone as well as the binding signatures of each ligand when the other binding site was occupied were measured. Different exosite 1 ligands with widely varied thermodynamic signatures cause a similar reduction in ΔH and a concomitantly lower entropy cost upon DAPA binding at the active site. The results suggest a general phenomenon of enthalpy-entropy compensation consistent with reduction of dynamics/increased folding of thrombin upon ligand binding to either the active site or exosite 1.

Field Tested Service Oriented Robotic Architecture: Case Study

Science.gov (United States)

Flueckiger, Lorenzo; Utz, Hanz

2012-01-01

This paper presents the lessons learned from six years of experiments with planetary rover prototypes running the Service Oriented Robotic Architecture (SORA) developed by the Intelligent Robotics Group (IRG) at NASA Ames Research Center. SORA relies on proven software methods and technologies applied to the robotic world. Based on a Service Oriented Architecture and robust middleware, SORA extends its reach beyond the on-board robot controller and supports the full suite of software tools used during mission scenarios from ground control to remote robotic sites. SORA has been field tested in numerous scenarios of robotic lunar and planetary exploration. The results of these high fidelity experiments are illustrated through concrete examples that have shown the benefits of using SORA as well as its limitations.

Prospective Architectures for Onboard vs Cloud-Based Decision Making for Unmanned Aerial Systems

Science.gov (United States)

Sankararaman, Shankar; Teubert, Christopher

2017-01-01

This paper investigates propsective architectures for decision-making in unmanned aerial systems. When these unmanned vehicles operate in urban environments, there are several sources of uncertainty that affect their behavior, and decision-making algorithms need to be robust to account for these different sources of uncertainty. It is important to account for several risk-factors that affect the flight of these unmanned systems, and facilitate decision-making by taking into consideration these various risk-factors. In addition, there are several technical challenges related to autonomous flight of unmanned aerial systems; these challenges include sensing, obstacle detection, path planning and navigation, trajectory generation and selection, etc. Many of these activities require significant computational power and in many situations, all of these activities need to be performed in real-time. In order to efficiently integrate these activities, it is important to develop a systematic architecture that can facilitate real-time decision-making. Four prospective architectures are discussed in this paper; on one end of the spectrum, the first architecture considers all activities/computations being performed onboard the vehicle whereas on the other end of the spectrum, the fourth and final architecture considers all activities/computations being performed in the cloud, using a new service known as Prognostics as a Service that is being developed at NASA Ames Research Center. The four different architectures are compared, their advantages and disadvantages are explained and conclusions are presented.

Walking through Architectural Spaces: The Impact of Interior Forms on Human Brain Dynamics.

Science.gov (United States)

Banaei, Maryam; Hatami, Javad; Yazdanfar, Abbas; Gramann, Klaus

2017-01-01

Neuroarchitecture uses neuroscientific tools to better understand architectural design and its impact on human perception and subjective experience. The form or shape of the built environment is fundamental to architectural design, but not many studies have shown the impact of different forms on the inhabitants' emotions. This study investigated the neurophysiological correlates of different interior forms on the perceivers' affective state and the accompanying brain activity. To understand the impact of naturalistic three-dimensional (3D) architectural forms, it is essential to perceive forms from different perspectives. We computed clusters of form features extracted from pictures of residential interiors and constructed exemplary 3D room models based on and representing different formal clusters. To investigate human brain activity during 3D perception of architectural spaces, we used a mobile brain/body imaging (MoBI) approach recording the electroencephalogram (EEG) of participants while they naturally walk through different interior forms in virtual reality (VR). The results revealed a strong impact of curvature geometries on activity in the anterior cingulate cortex (ACC). Theta band activity in ACC correlated with specific feature types ( r s (14) = 0.525, p = 0.037) and geometry ( r s (14) = -0.579, p = 0.019), providing evidence for a role of this structure in processing architectural features beyond their emotional impact. The posterior cingulate cortex and the occipital lobe were involved in the perception of different room perspectives during the stroll through the rooms. This study sheds new light on the use of mobile EEG and VR in architectural studies and provides the opportunity to study human brain dynamics in participants that actively explore and realistically experience architectural spaces.

Walking through Architectural Spaces: The Impact of Interior Forms on Human Brain Dynamics

Directory of Open Access Journals (Sweden)

Maryam Banaei

2017-09-01

Full Text Available Neuroarchitecture uses neuroscientific tools to better understand architectural design and its impact on human perception and subjective experience. The form or shape of the built environment is fundamental to architectural design, but not many studies have shown the impact of different forms on the inhabitants’ emotions. This study investigated the neurophysiological correlates of different interior forms on the perceivers’ affective state and the accompanying brain activity. To understand the impact of naturalistic three-dimensional (3D architectural forms, it is essential to perceive forms from different perspectives. We computed clusters of form features extracted from pictures of residential interiors and constructed exemplary 3D room models based on and representing different formal clusters. To investigate human brain activity during 3D perception of architectural spaces, we used a mobile brain/body imaging (MoBI approach recording the electroencephalogram (EEG of participants while they naturally walk through different interior forms in virtual reality (VR. The results revealed a strong impact of curvature geometries on activity in the anterior cingulate cortex (ACC. Theta band activity in ACC correlated with specific feature types (rs (14 = 0.525, p = 0.037 and geometry (rs (14 = −0.579, p = 0.019, providing evidence for a role of this structure in processing architectural features beyond their emotional impact. The posterior cingulate cortex and the occipital lobe were involved in the perception of different room perspectives during the stroll through the rooms. This study sheds new light on the use of mobile EEG and VR in architectural studies and provides the opportunity to study human brain dynamics in participants that actively explore and realistically experience architectural spaces.

Active-Site Hydration and Water Diffusion in Cytochrome P450cam: A Highly Dynamic Process

Energy Technology Data Exchange (ETDEWEB)

Miao, Yinglong [ORNL; Baudry, Jerome Y [ORNL

2011-01-01

Long-timescale molecular dynamics simulations (300 ns) are performed on both the apo- (i.e., camphor-free) and camphor-bound cytochrome P450cam (CYP101). Water diffusion into and out of the protein active site is observed without biased sampling methods. During the course of the molecular dynamics simulation, an average of 6.4 water molecules is observed in the camphor-binding site of the apo form, compared to zero water molecules in the binding site of the substrate-bound form, in agreement with the number of water molecules observed in crystal structures of the same species. However, as many as 12 water molecules can be present at a given time in the camphor-binding region of the active site in the case of apo-P450cam, revealing a highly dynamic process for hydration of the protein active site, with water molecules exchanging rapidly with the bulk solvent. Water molecules are also found to exchange locations frequently inside the active site, preferentially clustering in regions surrounding the water molecules observed in the crystal structure. Potential-of-mean-force calculations identify thermodynamically favored trans-protein pathways for the diffusion of water molecules between the protein active site and the bulk solvent. Binding of camphor in the active site modifies the free-energy landscape of P450cam channels toward favoring the diffusion of water molecules out of the protein active site.

Beyond Geodesign: The Architecture of Sitesynthesis

Directory of Open Access Journals (Sweden)

Bianco Lino

2017-12-01

Full Text Available This paper presents sustainable architecture as a function of sensory experience through time. Entitled sitesynthesis, this proposed approach forms the foundation of a holistic design methodology grounded physically in the geographical location of a given community and metaphysically in the spirit of place derived from sense experience. Sitesynthesis is made up of sitescapes which range from natural to cultural to social landscapes, themselves the product of the sensescapes through timescapes. The site is the place in geological, archaeological and historical time. The symbiotic relationship between the natural, cultural and social environs which sitesynthesis endeavours to attain is mutualistic rather than parasitic, a relationship from which society and the site both benefit rather than the former benefiting at the expense of the latter.

Active site mutations change the cleavage specificity of neprilysin.

Directory of Open Access Journals (Sweden)

Travis Sexton

Full Text Available Neprilysin (NEP, a member of the M13 subgroup of the zinc-dependent endopeptidase family is a membrane bound peptidase capable of cleaving a variety of physiological peptides. We have generated a series of neprilysin variants containing mutations at either one of two active site residues, Phe(563 and Ser(546. Among the mutants studied in detail we observed changes in their activity towards leucine(5-enkephalin, insulin B chain, and amyloid β(1-40. For example, NEP(F563I displayed an increase in preference towards cleaving leucine(5-enkephalin relative to insulin B chain, while mutant NEP(S546E was less discriminating than neprilysin. Mutants NEP(F563L and NEP(S546E exhibit different cleavage site preferences than neprilysin with insulin B chain and amyloid ß(1-40 as substrates. These data indicate that it is possible to alter the cleavage site specificity of neprilysin opening the way for the development of substrate specific or substrate exclusive forms of the enzyme with enhanced therapeutic potential.

Catalyst Architecture for Stable Single Atom Dispersion Enables Site-Specific Spectroscopic and Reactivity Measurements of CO Adsorbed to Pt Atoms, Oxidized Pt Clusters, and Metallic Pt Clusters on TiO2.

Science.gov (United States)

DeRita, Leo; Dai, Sheng; Lopez-Zepeda, Kimberly; Pham, Nicholas; Graham, George W; Pan, Xiaoqing; Christopher, Phillip

2017-10-11

Oxide-supported precious metal nanoparticles are widely used industrial catalysts. Due to expense and rarity, developing synthetic protocols that reduce precious metal nanoparticle size and stabilize dispersed species is essential. Supported atomically dispersed, single precious metal atoms represent the most efficient metal utilization geometry, although debate regarding the catalytic activity of supported single precious atom species has arisen from difficulty in synthesizing homogeneous and stable single atom dispersions, and a lack of site-specific characterization approaches. We propose a catalyst architecture and characterization approach to overcome these limitations, by depositing ∼1 precious metal atom per support particle and characterizing structures by correlating scanning transmission electron microscopy imaging and CO probe molecule infrared spectroscopy. This is demonstrated for Pt supported on anatase TiO 2 . In these structures, isolated Pt atoms, Pt iso , remain stable through various conditions, and spectroscopic evidence suggests Pt iso species exist in homogeneous local environments. Comparing Pt iso to ∼1 nm preoxidized (Pt ox ) and prereduced (Pt metal ) Pt clusters on TiO 2 , we identify unique spectroscopic signatures of CO bound to each site and find CO adsorption energy is ordered: Pt iso ≪ Pt metal atoms bonded to TiO 2 and that Pt iso exhibits optimal reactivity because every atom is exposed for catalysis and forms an interfacial site with TiO 2 . This approach should be generally useful for studying the behavior of supported precious metal atoms.

An open architecture for medical image workstation

Science.gov (United States)

Liang, Liang; Hu, Zhiqiang; Wang, Xiangyun

2005-04-01

Dealing with the difficulties of integrating various medical image viewing and processing technologies with a variety of clinical and departmental information systems and, in the meantime, overcoming the performance constraints in transferring and processing large-scale and ever-increasing image data in healthcare enterprise, we design and implement a flexible, usable and high-performance architecture for medical image workstations. This architecture is not developed for radiology only, but for any workstations in any application environments that may need medical image retrieving, viewing, and post-processing. This architecture contains an infrastructure named Memory PACS and different kinds of image applications built on it. The Memory PACS is in charge of image data caching, pre-fetching and management. It provides image applications with a high speed image data access and a very reliable DICOM network I/O. In dealing with the image applications, we use dynamic component technology to separate the performance-constrained modules from the flexibility-constrained modules so that different image viewing or processing technologies can be developed and maintained independently. We also develop a weakly coupled collaboration service, through which these image applications can communicate with each other or with third party applications. We applied this architecture in developing our product line and it works well. In our clinical sites, this architecture is applied not only in Radiology Department, but also in Ultrasonic, Surgery, Clinics, and Consultation Center. Giving that each concerned department has its particular requirements and business routines along with the facts that they all have different image processing technologies and image display devices, our workstations are still able to maintain high performance and high usability.

Modeling Architectural Patterns’ Behavior Using Architectural Primitives

NARCIS (Netherlands)

Waqas Kamal, Ahmad; Avgeriou, Paris

2008-01-01

Architectural patterns have an impact on both the structure and the behavior of a system at the architecture design level. However, it is challenging to model patterns’ behavior in a systematic way because modeling languages do not provide the appropriate abstractions and because each pattern

Vernacular Architecture of Northern Nigeria in the Light of Sustainability

Science.gov (United States)

Danja, Isa Ibrahim; Li, Xue; Dalibi, S. G.

2017-05-01

Nigeria, a country located in the Western part of Africa has been the site of numerous kingdoms and tribal states over the millennia and is divided in to Northern and Southern parts with the Hausa-Fulani’s, a diverse but culturally homogeneous people living in the northern part of Nigeria. The vernacular architecture of the Northern Nigeria (VANN) is perhaps one of the least known but most beautiful of the medieval age, over the centuries a tradition of fine architecture has flourished in the area, the variety and quality of buildings are bound to generate much delight and enthusiasm in anyone who is interested in buildings. Many of their early buildings such as mosques and palaces are bright and colorful. This architectural style is known as Tubali which means the traditional architecture in the Hausa language and globally known as Vernacular Architecture (VA). VANN has many unique features which distinguish it from any other architecture. All buildings, be it modern or old has an impact on the environment; hence the need for sustainable measures and practices regarding such buildings. The aim of this research paper is to examine the features of VANN in the light of sustainability. Reviewed literatures were used to identify the various features of the Vernacular Architecture in the Northern Nigeria which were critically examined in the light of sustainability and its concept. The result showcased the various features of VANN in the light of sustainability and also discussed the factors hindering the sustainable endeavors of VANN with respect to such features.

Genetic and Molecular Mechanisms of Quantitative Trait Loci Controlling Maize Inflorescence Architecture.

Science.gov (United States)

Li, Manfei; Zhong, Wanshun; Yang, Fang; Zhang, Zuxin

2018-03-01

The establishment of inflorescence architecture is critical for the reproduction of flowering plant species. The maize plant generates two types of inflorescences, the tassel and the ear, and their architectures have a large effect on grain yield and yield-related traits that are genetically controlled by quantitative trait loci (QTLs). Since ear and tassel architecture are deeply affected by the activity of inflorescence meristems, key QTLs and genes regulating meristematic activity have important impacts on inflorescence development and show great potential for optimizing grain yield. Isolation of yield trait-related QTLs is challenging, but these QTLs have direct application in maize breeding. Additionally, characterization and functional dissection of QTLs can provide genetic and molecular knowledge of quantitative variation in inflorescence architecture. In this review, we summarize currently identified QTLs responsible for the establishment of ear and tassel architecture and discuss the potential genetic control of four ear-related and four tassel-related traits. In recent years, several inflorescence architecture-related QTLs have been characterized at the gene level. We review the mechanisms of these characterized QTLs.

Space and Architecture's Current Line of Research? A Lunar Architecture Workshop With An Architectural Agenda.

Science.gov (United States)

Solomon, D.; van Dijk, A.

The "2002 ESA Lunar Architecture Workshop" (June 3-16) ESTEC, Noordwijk, NL and V2_Lab, Rotterdam, NL) is the first-of-its-kind workshop for exploring the design of extra-terrestrial (infra) structures for human exploration of the Moon and Earth-like planets introducing 'architecture's current line of research', and adopting an architec- tural criteria. The workshop intends to inspire, engage and challenge 30-40 European masters students from the fields of aerospace engineering, civil engineering, archi- tecture, and art to design, validate and build models of (infra) structures for Lunar exploration. The workshop also aims to open up new physical and conceptual terrain for an architectural agenda within the field of space exploration. A sound introduc- tion to the issues, conditions, resources, technologies, and architectural strategies will initiate the workshop participants into the context of lunar architecture scenarios. In my paper and presentation about the development of the ideology behind this work- shop, I will comment on the following questions: * Can the contemporary architectural agenda offer solutions that affect the scope of space exploration? It certainly has had an impression on urbanization and colonization of previously sparsely populated parts of Earth. * Does the current line of research in architecture offer any useful strategies for com- bining scientific interests, commercial opportunity, and public space? What can be learned from 'state of the art' architecture that blends commercial and public pro- grammes within one location? * Should commercial 'colonisation' projects in space be required to provide public space in a location where all humans present are likely to be there in a commercial context? Is the wave in Koolhaas' new Prada flagship store just a gesture to public space, or does this new concept in architecture and shopping evolve the public space? * What can we learn about designing (infra-) structures on the Moon or any other

Architecture-based Model for Preventive and Operative Crisis Management

National Research Council Canada - National Science Library

Jungert, Erland; Derefeldt, Gunilla; Hallberg, Jonas; Hallberg, Niklas; Hunstad, Amund; Thuren, Ronny

2004-01-01

.... A system that should support activities of this type must not only have a high capacity, with respect to the dataflow, but also have suitable tools for decision support. To overcome these problems, an architecture for preventive and operative crisis management is proposed. The architecture is based on models for command and control, but also for risk analysis.

A MCIN-based architecture of smart agriculture

Directory of Open Access Journals (Sweden)

Xiang Gu

2017-09-01

Full Text Available Purpose – Material conscious and information network (MCIN is a kind of cyber physics social system. This paper aims to study the MCIN modeling method and design the MCIN-based architecture of smart agriculture (MCIN-ASA which is different from current vertical architecture and involves production, management and commerce. Architecture is composed of three MCIN-ASA participants which are MCIN-ASA enterprises, individuals and commodity. Design/methodology/approach – Architecture uses enterprises and individuals personalized portals as the carriers which are linked precisely with each other through a peer-to-peer network called six-degrees-of-separation block-chain. The authors want to establish a self-organization, open and ecological operational system which includes active, personalized consumption, direct, centralized distribution, distributed and smart production. Findings – The paper models three main MCIN-ASA participants, namely, design the smart supply, demand and management functions, which show the feasibility innovation and high efficiency of implementing MCIN on agriculture. At the same time, the paper presents a prototype system based on the architecture. Originality/value – The authors think that MCIN-ASA improves current agriculture greatly and inspires a lot in production-marketing-combined electronic commerce.

A Study On The Influence Of Illuminance Quality To Student’s Performance Of Visual Activities: Case Study Of Architecture Studio Room In Universitas Islam Indonesia

OpenAIRE

Bayuaji, Wisnu Hendrawan; Ayu Iswardhani, Tanty Kesuma; Risky, Alif Angga

2017-01-01

This research aims to measure and evaluate the level of illuminance quality in architecture studio room in relation to the achievement of standard performance and minimum visual comfortableness that should be fulfilled. This study also explores the effect of both illumination quality and quantity to the students physical and psychological performance in conducting visual activities in the architecture studio room. This evaluation will also evaluate the deviation between perception of brightne...

Communication activities for NUMO's site selection process

International Nuclear Information System (INIS)

Takeuchi, Mitsuo; Okuyama, Shigeru; Kitayama, Kazumi; Kuba, Michiyoshi

2004-01-01

A siting program for geological disposal of high-level radioactive waste (HLW) in Japan has just started and is moving into a new stage of communication with the public. A final repository site will be selected via a stepwise process, as stipulated in the Specified Radioactive Waste Final Disposal Act promulgated in June 2000. Based on the Act, the site selection process of the Nuclear Waste Management Organization of Japan (NUMO, established in October 2000) will be carried out in the three steps: selection of Preliminary Investigation Areas (PIAs), selection of Detailed Investigation Areas (DIAs) and selection of the Repository Site. The Act also defines NUMO's responsibilities in terms of implementing the HLW disposal program in an open and transparent manner. NUMO fully understands the importance of public participation in its activities and is aiming to promote public involvement in the process of site selection based on a fundamental policy, which consists of 'adopting a stepwise approach', 'respecting the initiative of municipalities' and 'ensuring transparency in information disclosure'. This policy is clearly reflected in the adoption of an open solicitation approach for volunteer municipalities for Preliminary Investigation Areas (PIAs). NUMO made the official announcement of the start of its open solicitation program on 19 December 2002. This paper outlines how NUMO's activities are currently carried out with a view to encouraging municipalities to volunteer as PIAs and how public awareness of the safety of the HLW disposal is evaluated at this stage

Seismic activity parameters of the Finnish potential repository sites

International Nuclear Information System (INIS)

Saari, J.

2000-10-01

Posiva Oy has started a project for estimating the possible earthquake induced rock movements on the deposition holes containing canisters of spent nuclear fuel. These estimates will be made for the four investigation sites, Romuvaara, Kivetty, Olkiluoto and Haestholmen. This study deals with the current and future seismicity associated with the above mentioned sites. Seismic belts that participate the seismic behaviour of the studied sites have been identified and the magnitude-frequency distributions of these belts have been estimated. The seismic activity parameters of the sites have been deduced from the characteristics of the seismic belts in order to forecast the seismicity during the next 100,000 years. The report discusses the possible earthquakes induced by future glaciation. The seismic interpretation seems to indicate that the previous postglacial faults in Finnish Lapland have been generated in compressional environment. The orientation of the rather uniform compression has been NW-SE, which coincide with the current stress field. It seems that, although the impact of postglacial crustal rebound must have been significant, the impact of plate tectonics has been dominant. A major assumption of this study has been that future seismicity will generally resemble the current seismicity. However, when the postglacial seismicity is concerned, the magnitude-frequency distribution is likely different and the expected maximum magnitude will be higher. Maximum magnitudes of future postglacial earthquakes have been approximated by strain release examinations. Seismicity has been examined within the framework of the lineament maps, in order to associate the future significant earthquakes with active fault zones in the vicinity of the potential repository sites. (orig.)

Methodical Design of Software Architecture Using an Architecture Design Assistant (ArchE)

Science.gov (United States)

2005-04-01

PA 15213-3890 Methodical Design of Software Architecture Using an Architecture Design Assistant (ArchE) Felix Bachmann and Mark Klein Software...DATES COVERED 00-00-2005 to 00-00-2005 4. TITLE AND SUBTITLE Methodical Design of Software Architecture Using an Architecture Design Assistant...important for architecture design – quality requirements and constraints are most important Here’s some evidence: If the only concern is

Self-Assembled Supramolecular Architectures Lyotropic Liquid Crystals

CERN Document Server

Garti, Nissim

2012-01-01

This book will describe fundamentals and recent developments in the area of Self-Assembled Supramolecular Architecture and their relevance to the  understanding of the functionality of  membranes  as delivery systems for active ingredients. As the heirarchial architectures determine their performance capabilities, attention will be paid to theoretical and design aspects related to the construction of lyotropic liquid crystals: mesophases such as lamellar, hexagonal, cubic, sponge phase micellosomes. The book will bring to the reader mechanistic aspects, compositional c

Mars Sample Return Architecture Assessment Study

Science.gov (United States)

Centuori, S.; Hermosín, P.; Martín, J.; De Zaiacomo, G.; Colin, S.; Godfrey, A.; Myles, J.; Johnson, H.; Sachdev, T.; Ahmed, R.

2018-04-01

Current paper presents the results of ESA funded activity "Mars Sample Return Architecture Assessment Study" carried-out by DEIMOS Space, Lockheed Martin UK Ampthill, and MDA Corporation, where more than 500 mission design options have been studied.

Preparation and Use of Photocatalytically Active Segmented Ag|ZnO and Coaxial TiO2-Ag Nanowires Made by Templated Electrodeposition

NARCIS (Netherlands)

Maijenburg, A.W.; Rodijk, E.J.B.; Maas, M.G.; ten Elshof, Johan E.

2014-01-01

Photocatalytically active nanostructures require a large specific surface area with the presence of many catalytically active sites for the oxidation and reduction half reactions, and fast electron (hole) diffusion and charge separation. Nanowires present suitable architectures to meet these

Open architecture of smart sensor suites

Science.gov (United States)

Müller, Wilmuth; Kuwertz, Achim; Grönwall, Christina; Petersson, Henrik; Dekker, Rob; Reinert, Frank; Ditzel, Maarten

2017-10-01

Experiences from recent conflicts show the strong need for smart sensor suites comprising different multi-spectral imaging sensors as core elements as well as additional non-imaging sensors. Smart sensor suites should be part of a smart sensor network - a network of sensors, databases, evaluation stations and user terminals. Its goal is to optimize the use of various information sources for military operations such as situation assessment, intelligence, surveillance, reconnaissance, target recognition and tracking. Such a smart sensor network will enable commanders to achieve higher levels of situational awareness. Within the study at hand, an open system architecture was developed in order to increase the efficiency of sensor suites. The open system architecture for smart sensor suites, based on a system-of-systems approach, enables combining different sensors in multiple physical configurations, such as distributed sensors, co-located sensors combined in a single package, tower-mounted sensors, sensors integrated in a mobile platform, and trigger sensors. The architecture was derived from a set of system requirements and relevant scenarios. Its mode of operation is adaptable to a series of scenarios with respect to relevant objects of interest, activities to be observed, available transmission bandwidth, etc. The presented open architecture is designed in accordance with the NATO Architecture Framework (NAF). The architecture allows smart sensor suites to be part of a surveillance network, linked e.g. to a sensor planning system and a C4ISR center, and to be used in combination with future RPAS (Remotely Piloted Aircraft Systems) for supporting a more flexible dynamic configuration of RPAS payloads.

Seasonal effects on digging activity and burrow architecture in the ...

African Journals Online (AJOL)

Most polygynous male mammals exhibit little or no parental care or involvement raising young. Instead, they invest indirectly in their own morphological and physiological attributes which enhance their chance of reproduction. Such secondary morphological sex traits may contribute to differences in the burrow architecture ...

An Introduction to Architectural Surety(SM) Education

Energy Technology Data Exchange (ETDEWEB)

Matalucci, R.V.; Miyoshi, D.S.

1998-09-01

This report describes the Sandia activities in the developing field management approach to enhancing National Laboratories (Sandia) educational outreach of architectural and infrastructure surety, a risk the safety, security, and reliability of facilities, systems, and structures. It begins with a description of the field of architectural and infrastmcture surety, including Sandia's historical expertise and experience in nuclear weapons surety. An overview of the 1996 Sandia Workshop on Architectural SuretysM is then provided to reference the initiation of the various activities. This workshop established the need for a surety education program at the University level and recommended that Sandia develop the course material as soon as possible. Technical material was assembled and the course was offered at the University of New Mexico (UNM) during the 1997 spring semester. The bulk of this report accordingly summarizes the lecture material presented in this pioneering graduate-level course on Infrastructure Surety in the Civil Engineering Department at UNM. This groundbreaking class presented subject matter developed by experts from Sandia, and included additional information from guest lecturers from academia, government, and industry. Also included in this report are summaries of the term projects developed by the graduate students, an overview of the 1997 International Conference on Architectural Suretp: Assuring the Performance of Buildings and Injiastruchwes (co-sponsored by Sandia, the American Institute of Architects, and the American Society of Civil Engineers), and recommendations for further course work development. The U.S. Department of Energy provides support to this emerging field of architectural and infrastructure surety and recognizes its broad application to developing government, industry, and professional standards in the national interest.

Practical, redundant, failure-tolerant, self-reconfiguring embedded system architecture

Science.gov (United States)

Klarer, Paul R.; Hayward, David R.; Amai, Wendy A.

2006-10-03

This invention relates to system architectures, specifically failure-tolerant and self-reconfiguring embedded system architectures. The invention provides both a method and architecture for redundancy. There can be redundancy in both software and hardware for multiple levels of redundancy. The invention provides a self-reconfiguring architecture for activating redundant modules whenever other modules fail. The architecture comprises: a communication backbone connected to two or more processors and software modules running on each of the processors. Each software module runs on one processor and resides on one or more of the other processors to be available as a backup module in the event of failure. Each module and backup module reports its status over the communication backbone. If a primary module does not report, its backup module takes over its function. If the primary module becomes available again, the backup module returns to its backup status.

Architectural freedom and industrialised architecture

DEFF Research Database (Denmark)

Vestergaard, Inge

2012-01-01

to the building physic problems a new industrialized period has started based on light weight elements basically made of wooden structures, faced with different suitable materials meant for individual expression for the specific housing area. It is the purpose of this article to widen up the different design...... to this systematic thinking of the building technique we get a diverse and functional architecture. Creating a new and clearer story telling about new and smart system based thinking behind the architectural expression....

Research of Ancient Architectures in Jin-Fen Area Based on GIS&BIM Technology

Science.gov (United States)

Jia, Jing; Zheng, Qiuhong; Gao, Huiying; Sun, Hai

2017-05-01

The number of well-preserved ancient buildings located in Shanxi Province, enjoying the absolute maximum proportion of ancient architectures in China, is about 18418, among which, 9053 buildings have the structural style of wood frame. The value of the application of BIM (Building Information Modeling) and GIS (Geographic Information System) is gradually probed and testified in the corresponding fields of ancient architecture’s spatial distribution information management, routine maintenance and special conservation & restoration, the evaluation and simulation of related disasters, such as earthquake. The research objects are ancient architectures in JIN-FEN area, which were first investigated by Sicheng LIANG and recorded in his work of “Chinese ancient architectures survey report”. The research objects, i.e. the ancient architectures in Jin-Fen area include those in Sicheng LIANG’s investigation, and further adjustments were made through authors’ on-site investigation and literature searching & collection. During this research process, the spatial distributing Geodatabase of research objects is established utilizing GIS. The BIM components library for ancient buildings is formed combining on-site investigation data and precedent classic works, such as “Yingzao Fashi”, a treatise on architectural methods in Song Dynasty, “Yongle Encyclopedia” and “Gongcheng Zuofa Zeli”, case collections of engineering practice, by the Ministry of Construction of Qing Dynasty. A building of Guangsheng temple in Hongtong county is selected as an example to elaborate the BIM model construction process based on the BIM components library for ancient buildings. Based on the foregoing work results of spatial distribution data, attribute data of features, 3D graphic information and parametric building information model, the information management system for ancient architectures in Jin-Fen Area, utilizing GIS&BIM technology, could be constructed to support the

Preemptive Architecture: Explosive Art and Future Architectures in Cursed Urban Zones

Directory of Open Access Journals (Sweden)

Stahl Stenslie

2017-04-01

Full Text Available This article describes the art and architectural research project Preemptive Architecture that uses artistic strategies and approaches to create bomb-ready architectural structures that act as instruments for the undoing of violence in war. Increasing environmental usability through destruction represents an inverse strategy that reverses common thinking patterns about warfare, art and architecture. Building structures predestined for a construc­tive destruction becomes a creative act. One of the main motivations behind this paper is to challenge and expand the material thinking as well as the socio-political conditions related to artistic, architectural and design based practices.   Article received: December 12, 2016; Article accepted: January 10, 2017; Published online: April 20, 2017 Original scholarly paper How to cite this article: Stenslie, Stahl, and Magne Wiggen. "Preemptive Architecture: Explosive Art and Future Architectures in Cursed Urban Zones." AM Journal of Art and Media Studies 12 (2017: 29-39. doi: 10.25038/am.v0i12.165

Enterprise architecture patterns practical solutions for recurring IT-architecture problems

CERN Document Server

Perroud, Thierry

2013-01-01

Every enterprise architect faces similar problems when designing and governing the enterprise architecture of a medium to large enterprise. Design patterns are a well-established concept in software engineering, used to define universally applicable solution schemes. By applying this approach to enterprise architectures, recurring problems in the design and implementation of enterprise architectures can be solved over all layers, from the business layer to the application and data layer down to the technology layer.Inversini and Perroud describe patterns at the level of enterprise architecture

MUF architecture /art London

DEFF Research Database (Denmark)

Svenningsen Kajita, Heidi

2009-01-01

Om MUF architecture samt interview med Liza Fior og Katherine Clarke, partnere i muf architecture/art......Om MUF architecture samt interview med Liza Fior og Katherine Clarke, partnere i muf architecture/art...

The Rhetoric of Campus Architecture

Science.gov (United States)

Smith, Cynthia Duquette

2016-01-01

The group activity described in this article was originally designed for an upper-division undergraduate course on Rhetoric and Architecture, but would also be well suited for courses in Persuasion, Rhetorical Criticism, or Visual Rhetoric. Any undergraduate course related to communication and design (including Advertising) could make excellent…

Do Changes in Muscle Architecture Affect Post-Activation Potentiation?

Directory of Open Access Journals (Sweden)

Danielle Reardon, Jay R. Hoffman, Gerald T. Mangine, Adam J. Wells, Adam M. Gonzalez, Adam R. Jajtner, Jeremy R. Townsend, William P. McCormack, Jeffrey R. Stout, Maren S. Fragala, David H. Fukuda

2014-09-01

Full Text Available The purpose of this randomized, cross-over design study was to examine the effect of three different muscle potentiation protocols on acute changes in muscle architecture and vertical jump performance. Eleven experienced, resistance trained men (25.2±3.6y completed three potentiation squat protocols using moderate intensity (MI; 75%, 3 sets x 10 repetitions, high intensity (HI; 90%, 3 sets x 3 repetitions and 100% (1RM; 1 set x 1repetition of their 1RM. In addition, all participants completed a control session (CTL in which no protocol was performed. During each testing session, muscle architecture and vertical jump testing were assessed at baseline (BL, 8min post (8P and 20min post (20P workout. Ultrasound measures included cross sectional area (CSA and pennation angle (PANG of both the rectus femoris (RF and vastus lateralis (VL. Following each ultrasound measure, peak vertical jump power (PVJP and mean (MVJP power was assessed using an accelerometer. Magnitude based inferences were used to make comparisons between trials. The MI trial resulted in a likely greater increase from BL to 8P and 20P in RF-CSA and VL-CSA, while the HI trial resulted in a likely greater change from BL to 20P in both RF-CSA and VL-CSA. Meanwhile, changes in PVJP and MVJP for the MI trial was likely decreased at BL-8P and BL–20P, while the HI trial was shown to result in a likely or possible decrease compared to CTL at BL-8P and BL–20P, respectively. A likely negative relationship was observed between changes in VL-PANG and MVJP (r = -0.35; p , 0.018 at BL-8P, and between changes in PVJP and RF-CSA (r = -0.37; p , 0.014 at BL–20P. Results of this study were unable to demonstrate any potentiation response from the trials employed, however these protocols did result in acute muscle architectural changes.

Tuning complement activation and pathway through controlled molecular architecture of dextran chains in nanoparticle corona.

Science.gov (United States)

Coty, Jean-Baptiste; Eleamen Oliveira, Elquio; Vauthier, Christine

2017-11-05

The understanding of complement activation by nanomaterials is a key to a rational design of safe and efficient nanomedicines. This work proposed a systematic study investigating how molecular design of nanoparticle coronas made of dextran impacts on mechanisms that trigger complement activation. The nanoparticles used for this work consisted of dextran-coated poly(isobutylcyanoacrylate) (PIBCA) nanoparticles have already been thoroughly characterized. Their different capacity to trigger complement activation established on the cleavage of the protein C3 was also already described making these nanoparticles good models to investigate the relation between the molecular feature of their corona and the mechanism by which they triggered complement activation. Results of this new study show that complement activation pathways can be selected by distinct architectures formed by dextran chains composing the nanoparticle corona. Assumptions that explain the relation between complement activation mechanisms triggered by the nanoparticles and the nanoparticle corona molecular feature were proposed. These results are of interest to better understand how the design of dextran-coated nanomaterials will impact interactions with the complement system. It can open perspectives with regard to the selection of a preferential complement activation pathway or prevent the nanoparticles to activate the complement system, based on a rational choice of the corona configuration. Copyright © 2017 Elsevier B.V. All rights reserved.

Structural Basis for the Inhibition of RNase H Activity of HIV-1 Reverse Transcriptase by RNase H Active Site-Directed Inhibitors

Energy Technology Data Exchange (ETDEWEB)

Su, Hua-Poo; Yan, Youwei; Prasad, G. Sridhar; Smith, Robert F.; Daniels, Christopher L.; Abeywickrema, Pravien D.; Reid, John C.; Loughran, H. Marie; Kornienko, Maria; Sharma, Sujata; Grobler, Jay A.; Xu, Bei; Sardana, Vinod; Allison, Timothy J.; Williams, Peter D.; Darke, Paul L.; Hazuda, Daria J.; Munshi, Sanjeev (Merck)

2010-09-02

HIV/AIDS continues to be a menace to public health. Several drugs currently on the market have successfully improved the ability to manage the viral burden in infected patients. However, new drugs are needed to combat the rapid emergence of mutated forms of the virus that are resistant to existing therapies. Currently, approved drugs target three of the four major enzyme activities encoded by the virus that are critical to the HIV life cycle. Although a number of inhibitors of HIV RNase H activity have been reported, few inhibit by directly engaging the RNase H active site. Here, we describe structures of naphthyridinone-containing inhibitors bound to the RNase H active site. This class of compounds binds to the active site via two metal ions that are coordinated by catalytic site residues, D443, E478, D498, and D549. The directionality of the naphthyridinone pharmacophore is restricted by the ordering of D549 and H539 in the RNase H domain. In addition, one of the naphthyridinone-based compounds was found to bind at a second site close to the polymerase active site and non-nucleoside/nucleotide inhibitor sites in a metal-independent manner. Further characterization, using fluorescence-based thermal denaturation and a crystal structure of the isolated RNase H domain reveals that this compound can also bind the RNase H site and retains the metal-dependent binding mode of this class of molecules. These structures provide a means for structurally guided design of novel RNase H inhibitors.

LC Data QUEST: A Technical Architecture for Community Federated Clinical Data Sharing.

Science.gov (United States)

Stephens, Kari A; Lin, Ching-Ping; Baldwin, Laura-Mae; Echo-Hawk, Abigail; Keppel, Gina A; Buchwald, Dedra; Whitener, Ron J; Korngiebel, Diane M; Berg, Alfred O; Black, Robert A; Tarczy-Hornoch, Peter

2012-01-01

The University of Washington Institute of Translational Health Sciences is engaged in a project, LC Data QUEST, building data sharing capacity in primary care practices serving rural and tribal populations in the Washington, Wyoming, Alaska, Montana, Idaho region to build research infrastructure. We report on the iterative process of developing the technical architecture for semantically aligning electronic health data in primary care settings across our pilot sites and tools that will facilitate linkages between the research and practice communities. Our architecture emphasizes sustainable technical solutions for addressing data extraction, alignment, quality, and metadata management. The architecture provides immediate benefits to participating partners via a clinical decision support tool and data querying functionality to support local quality improvement efforts. The FInDiT tool catalogues type, quantity, and quality of the data that are available across the LC Data QUEST data sharing architecture. These tools facilitate the bi-directional process of translational research.

ELISA, a demonstrator environment for information systems architecture design

Science.gov (United States)

Panem, Chantal

1994-01-01

This paper describes an approach of reusability of software engineering technology in the area of ground space system design. System engineers have lots of needs similar to software developers: sharing of a common data base, capitalization of knowledge, definition of a common design process, communication between different technical domains. Moreover system designers need to simulate dynamically their system as early as possible. Software development environments, methods and tools now become operational and widely used. Their architecture is based on a unique object base, a set of common management services and they host a family of tools for each life cycle activity. In late '92, CNES decided to develop a demonstrative software environment supporting some system activities. The design of ground space data processing systems was chosen as the application domain. ELISA (Integrated Software Environment for Architectures Specification) was specified as a 'demonstrator', i.e. a sufficient basis for demonstrations, evaluation and future operational enhancements. A process with three phases was implemented: system requirements definition, design of system architectures models, and selection of physical architectures. Each phase is composed of several activities that can be performed in parallel, with the provision of Commercial Off the Shelves Tools. ELISA has been delivered to CNES in January 94, currently used for demonstrations and evaluations on real projects (e.g. SPOT4 Satellite Control Center). It is on the way of new evolutions.

VERNACULAR ARCHITECTURE: AN INTRODUCTORY COURSE TO LEARN ARCHITECTURE IN INDIA

Directory of Open Access Journals (Sweden)

Miki Desai

2010-07-01

Full Text Available “The object in view of both my predecessors in office and by myself has been rather to bring out the reasoning powers of individual students, so that they may understand the inner meaning of the old forms and their original function and may develop and modernize and gradually produce an architecture, Indian in character, but at the same time as suited to present day India as the old styles were to their own times and environment.” Claude Batley-1940; Lang, Desai, Desai, 1997 (p.143. The article introduces teaching philosophy, content and method of Basic Design I and II for first year students of architecture at the Faculty of Architecture, Centre for Environmental Planning and Technology (CEPT University, Ahmedabad, India. It is framed within the Indian perspective of architectural education from the British colonial times. Commencing with important academic literature and biases of the initial colonial period, it quickly traces architectural education in CEPT, the sixteenth school of post-independent India, set up in 1962, discussing the foundation year teaching imparted. The school was Modernist and avant-garde. The author introduced these two courses against the back drop of the Universalist Modernist credo of architecture and education. In the courses, the primary philosophy behind learning design emerges from heuristic method. The aim of the first course is seen as infusing interest in visual world, development of manual skills and dexterity through the dictum of ‘Look-feel-reason out-evaluate’ and ‘observe-record-interpret-synthesize transform express’. Due to the lack of architectural orientation in Indian schooling; the second course assumes vernacular architecture as a reasonable tool for a novice to understand the triangular relationship of society, architecture and physical context and its impact on design. The students are analytically exposed to the regional variety of architectures logically stemming from the geo

A source of energy : sustainable architecture and urbanism

Energy Technology Data Exchange (ETDEWEB)

Roestvik, Harald N.

2011-07-01

An update on the environmental challenges. Meant to inspire and be a source of energy.Tearing down myths and floodlighting paradoxes. Particularly relevant for students of architecture, architects and concerned citizens. Training tasks, recommendations for further source books and web sites, are included. From the content: Climate change and consensus, Population growth, Food production, The sustainable city, Transportation myths and facts, A mini history of environmental architecture, Architects' approach to sustainable design, The failure of western architects; a case study; China, The passive, zeb and plus energy building, Natural ventilation, Sustainable materials, Plastics in building, Nuclear energy, Solar energy, The grid of the future, Indoor climate and health. The sick building syndrome, Radon, Universal design, Paradoxes, Bullying techniques, Trust yourself, Timing, Which gateway will you choose?, On transience. (au)

A Control Architecture to Coordinate Distributed Generators and Active Power Filters Coexisting in a Microgrid

DEFF Research Database (Denmark)

Hashempour, Mohammad M.; Firoozabadi, Mehdi Savaghebi; Quintero, Juan Carlos Vasquez

2016-01-01

This paper proposes a control architecture of distributed generators (DGs) inverters and shunt active power filters (APFs) in microgrids to compensate voltage harmonics in a coordinated way. For this, a hierarchical control structure is proposed that includes two control levels. The primary (local......) control consists of power controllers, selective virtual impedance loops and proportional-resonant (PR) voltage/current controllers. The secondary (central) control manages the compensation level of voltage harmonic distortion of sensitive load bus (SLB). Compensation of SLB harmonics by control of DGs...... excessive harmonics or overloading of interface inverters. Effectiveness of the proposed control scheme is demonstrated through simulation studies....

A control architecture to coordinate distributed generators and active power filters coexisting in a microgrid

DEFF Research Database (Denmark)

Hashempour, Mohammad M.; Savaghebi, Mehdi; Quintero, Juan Carlos Vasquez

2016-01-01

This paper proposes a control architecture of distributed generators (DGs) inverters and shunt active power filters (APFs) in microgrids to compensate voltage harmonics in a coordinated way. For this, a hierarchical control structure is proposed that includes two control levels. The primary (local......) control consists of power controllers, selective virtual impedance loops and proportional-resonant (PR) voltage/current controllers. The secondary (central) control manages the compensation level of voltage harmonic distortion of sensitive load bus (SLB). Compensation of SLB harmonics by control of DGs...... excessive harmonics or overloading of interface inverters. Effectiveness of the proposed control scheme is demonstrated through simulation studies....

Architecture and Film

OpenAIRE

Mohammad Javaheri, Saharnaz

2016-01-01

Film does not exist without architecture. In every movie that has ever been made throughout history, the cinematic image of architecture is embedded within the picture. Throughout my studies and research, I began to see that there is no director who can consciously or unconsciously deny the use of architectural elements in his or her movies. Architecture offers a strong profile to distinguish characters and story. In the early days, films were shot in streets surrounde...

Architecture for robot intelligence

Science.gov (United States)

Peters, II, Richard Alan (Inventor)

2004-01-01

An architecture for robot intelligence enables a robot to learn new behaviors and create new behavior sequences autonomously and interact with a dynamically changing environment. Sensory information is mapped onto a Sensory Ego-Sphere (SES) that rapidly identifies important changes in the environment and functions much like short term memory. Behaviors are stored in a DBAM that creates an active map from the robot's current state to a goal state and functions much like long term memory. A dream state converts recent activities stored in the SES and creates or modifies behaviors in the DBAM.

Architectural geometry

KAUST Repository

Pottmann, Helmut

2014-11-26

Around 2005 it became apparent in the geometry processing community that freeform architecture contains many problems of a geometric nature to be solved, and many opportunities for optimization which however require geometric understanding. This area of research, which has been called architectural geometry, meanwhile contains a great wealth of individual contributions which are relevant in various fields. For mathematicians, the relation to discrete differential geometry is significant, in particular the integrable system viewpoint. Besides, new application contexts have become available for quite some old-established concepts. Regarding graphics and geometry processing, architectural geometry yields interesting new questions but also new objects, e.g. replacing meshes by other combinatorial arrangements. Numerical optimization plays a major role but in itself would be powerless without geometric understanding. Summing up, architectural geometry has become a rewarding field of study. We here survey the main directions which have been pursued, we show real projects where geometric considerations have played a role, and we outline open problems which we think are significant for the future development of both theory and practice of architectural geometry.

Architectural geometry

KAUST Repository

Pottmann, Helmut; Eigensatz, Michael; Vaxman, Amir; Wallner, Johannes

2014-01-01

Around 2005 it became apparent in the geometry processing community that freeform architecture contains many problems of a geometric nature to be solved, and many opportunities for optimization which however require geometric understanding. This area of research, which has been called architectural geometry, meanwhile contains a great wealth of individual contributions which are relevant in various fields. For mathematicians, the relation to discrete differential geometry is significant, in particular the integrable system viewpoint. Besides, new application contexts have become available for quite some old-established concepts. Regarding graphics and geometry processing, architectural geometry yields interesting new questions but also new objects, e.g. replacing meshes by other combinatorial arrangements. Numerical optimization plays a major role but in itself would be powerless without geometric understanding. Summing up, architectural geometry has become a rewarding field of study. We here survey the main directions which have been pursued, we show real projects where geometric considerations have played a role, and we outline open problems which we think are significant for the future development of both theory and practice of architectural geometry.

Integrating Legacy Data into a New Method for Studying Architecture: A case study from Isthmia, Greece

Directory of Open Access Journals (Sweden)

Steven J.R. Ellis

2008-06-01

Full Text Available This article outlines a new methodology developed to disentangle the hitherto incomprehensible maze of poorly preserved architecture at the Archaic through late Roman period Panhellenic sanctuary at Isthmia, Greece, into clearly defined buildings with their relative construction phases. The methodology combines on-site architectural analyses with the digitisation and reintegration of the site's legacy data within a GIS. The results of this study, still in its preliminary stages, reveal an area east of the Temple of Poseidon at Isthmia as a built environment of rather large and complex units in contrast to the conventional interpretation of a series of small and unimportant structures.

Elements of Architecture

DEFF Research Database (Denmark)

Elements of Architecture explores new ways of engaging architecture in archaeology. It conceives of architecture both as the physical evidence of past societies and as existing beyond the physical environment, considering how people in the past have not just dwelled in buildings but have existed...

Digitally-Driven Architecture

Directory of Open Access Journals (Sweden)

Henriette Bier

2014-07-01

Full Text Available The shift from mechanical to digital forces architects to reposition themselves: Architects generate digital information, which can be used not only in designing and fabricating building components but also in embedding behaviours into buildings. This implies that, similar to the way that industrial design and fabrication with its concepts of standardisation and serial production influenced modernist architecture, digital design and fabrication influences contemporary architecture. While standardisation focused on processes of rationalisation of form, mass-customisation as a new paradigm that replaces mass-production, addresses non-standard, complex, and flexible designs. Furthermore, knowledge about the designed object can be encoded in digital data pertaining not just to the geometry of a design but also to its physical or other behaviours within an environment. Digitally-driven architecture implies, therefore, not only digitally-designed and fabricated architecture, it also implies architecture – built form – that can be controlled, actuated, and animated by digital means.In this context, this sixth Footprint issue examines the influence of digital means as pragmatic and conceptual instruments for actuating architecture. The focus is not so much on computer-based systems for the development of architectural designs, but on architecture incorporating digital control, sens­ing, actuating, or other mechanisms that enable buildings to inter­act with their users and surroundings in real time in the real world through physical or sensory change and variation.

HISTORY AND ARCHITECTURE OF FORTRESS "ANEVSKO KALE"

Directory of Open Access Journals (Sweden)

Maria Nikolova

2016-12-01

Full Text Available Оne of the preserved monuments of Bulgarian history is presented, undergone changes over the years and containing the rich history that could be of interest to different age groups. The details about the complex architecture and lifestyle of our ancestors add more opportunities for discussions and lectures. The location close to historic towns and monuments is also one of the advantages of the site for development and marketing of tourist and guide services.

Settings for Physical Activity – Developing a Site-specific Physical Activity Behavior Model based on Multi-level Intervention Studies

DEFF Research Database (Denmark)

Troelsen, Jens; Klinker, Charlotte Demant; Breum, Lars

Settings for Physical Activity – Developing a Site-specific Physical Activity Behavior Model based on Multi-level Intervention Studies Introduction: Ecological models of health behavior have potential as theoretical framework to comprehend the multiple levels of factors influencing physical...... to be taken into consideration. A theoretical implication of this finding is to develop a site-specific physical activity behavior model adding a layered structure to the ecological model representing the determinants related to the specific site. Support: This study was supported by TrygFonden, Realdania...... activity (PA). The potential is shown by the fact that there has been a dramatic increase in application of ecological models in research and practice. One proposed core principle is that an ecological model is most powerful if the model is behavior-specific. However, based on multi-level interventions...

Synthesis of a molecularly defined single-active site heterogeneous catalyst for selective oxidation of N-heterocycles.

Science.gov (United States)

Zhang, Yujing; Pang, Shaofeng; Wei, Zhihong; Jiao, Haijun; Dai, Xingchao; Wang, Hongli; Shi, Feng

2018-04-13

Generally, a homogeneous catalyst exhibits good activity and defined active sites but it is difficult to recycle. Meanwhile, a heterogeneous catalyst can easily be reused but its active site is difficult to reveal. It is interesting to bridge the gap between homogeneous and heterogeneous catalysis via controllable construction of a heterogeneous catalyst containing defined active sites. Here, we report that a molecularly defined, single-active site heterogeneous catalyst has been designed and prepared via the oxidative polymerization of maleimide derivatives. These polymaleimide derivatives can be active catalysts for the selective oxidation of heterocyclic compounds to quinoline and indole via the recycling of -C=O and -C-OH groups, which was confirmed by tracing the reaction with GC-MS using maleimide as the catalyst and by FT-IR analysis with polymaleimide as the catalyst. These results might promote the development of heterogeneous catalysts with molecularly defined single active sites exhibiting a comparable activity to homogeneous catalysts.

The status of siting activities for a low level waste repository in the Philippines

International Nuclear Information System (INIS)

Valdezco, E.M.; Visitacion, M.; Palattao, B.; Marcelo, E.A.; Venida, L.L.

2001-01-01

The process of site selection for a low level waste repository was initiated in 1976 when the Philippine Government decided to go nuclear and constructed the first Philippine Nuclear Power Plant in the Bataan Peninsula. However, all siting activities were suspended when the nuclear power plant was mothballed and the final decision was made to convert the plant into a combined cycle power plant. In 1995, an inter-agency committee was created under the Nuclear Power Steering Committee and mandated to conduct studies on siting of radioactive waste disposal facilities, and at the same time, perform R and D activities in support of the project. This paper describes the various siting activities carried out to date. (author)

Biological role of site-specific O-glycosylation in cell adhesion activity and phosphorylation of osteopontin.

Science.gov (United States)

Oyama, Midori; Kariya, Yoshinobu; Kariya, Yukiko; Matsumoto, Kana; Kanno, Mayumi; Yamaguchi, Yoshiki; Hashimoto, Yasuhiro

2018-05-09

Osteopontin (OPN) is an extracellular glycosylated phosphoprotein that promotes cell adhesion by interacting with several integrin receptors. We previously reported that an OPN mutant lacking five O-glycosylation sites (Thr 134 /Thr 138 /Thr 143 /Thr 147 /Thr 152 ) in the threonine/proline-rich region increased cell adhesion activity and phosphorylation compared with the wild type. However, the role of O-glycosylation in cell adhesion activity and phosphorylation of OPN remains to be clarified. Here, we show that site-specific O-glycosylation in the threonine/proline-rich region of OPN affects its cell adhesion activity and phosphorylation independently and/or synergistically. Using site-directed mutagenesis, we found that OPN mutants with substitution sets of Thr 134 /Thr 138 or Thr 143 /Thr 147 /Thr 152 had decreased and increased cell adhesion activity, respectively. In contrast, the introduction of a single mutation into the O-glycosylation sites had no effect on OPN cell adhesion activity. An adhesion assay using function-blocking antibodies against αvβ3 and β1 integrins, as well as αvβ3 integrin-overexpressing A549 cells, revealed that site-specific O-glycosylation affected the association of OPN with the two integrins. Phosphorylation analyses using phos-tag and LC-MS/MS indicated that phosphorylation levels and sites were influenced by the O-glycosylation status, although the number of O-glycosylation sites was not correlated with the phosphorylation level in OPN. Furthermore, a correlation analysis between phosphorylation level and cell adhesion activity in OPN mutants with the site-specific O-glycosylation showed that they were not always correlated. These results provide conclusive evidence of a novel regulatory mechanism of cell adhesion activity and phosphorylation of OPN by site-specific O-glycosylation. © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

PICNIC Architecture.

Science.gov (United States)

Saranummi, Niilo

2005-01-01

The PICNIC architecture aims at supporting inter-enterprise integration and the facilitation of collaboration between healthcare organisations. The concept of a Regional Health Economy (RHE) is introduced to illustrate the varying nature of inter-enterprise collaboration between healthcare organisations collaborating in providing health services to citizens and patients in a regional setting. The PICNIC architecture comprises a number of PICNIC IT Services, the interfaces between them and presents a way to assemble these into a functioning Regional Health Care Network meeting the needs and concerns of its stakeholders. The PICNIC architecture is presented through a number of views relevant to different stakeholder groups. The stakeholders of the first view are national and regional health authorities and policy makers. The view describes how the architecture enables the implementation of national and regional health policies, strategies and organisational structures. The stakeholders of the second view, the service viewpoint, are the care providers, health professionals, patients and citizens. The view describes how the architecture supports and enables regional care delivery and process management including continuity of care (shared care) and citizen-centred health services. The stakeholders of the third view, the engineering view, are those that design, build and implement the RHCN. The view comprises four sub views: software engineering, IT services engineering, security and data. The proposed architecture is founded into the main stream of how distributed computing environments are evolving. The architecture is realised using the web services approach. A number of well established technology platforms and generic standards exist that can be used to implement the software components. The software components that are specified in PICNIC are implemented in Open Source.

A Scalable Architecture for VoIP Conferencing

Directory of Open Access Journals (Sweden)

R Venkatesha Prasad

2003-10-01

Full Text Available Real-Time services are traditionally supported on circuit switched network. However, there is a need to port these services on packet switched network. Architecture for audio conferencing application over the Internet in the light of ITU-T H.323 recommendations is considered. In a conference, considering packets only from a set of selected clients can reduce speech quality degradation because mixing packets from all clients can lead to lack of speech clarity. A distributed algorithm and architecture for selecting clients for mixing is suggested here based on a new quantifier of the voice activity called "Loudness Number" (LN. The proposed system distributes the computation load and reduces the load on client terminals. The highlights of this architecture are scalability, bandwidth saving and speech quality enhancement. Client selection for playing out tries to mimic a physical conference where the most vocal participants attract more attention. The contributions of the paper are expected to aid H.323 recommendations implementations for Multipoint Processors (MP. A working prototype based on the proposed architecture is already functional.

Satellite ATM Networks: Architectures and Guidelines Developed

Science.gov (United States)

vonDeak, Thomas C.; Yegendu, Ferit

1999-01-01

An important element of satellite-supported asynchronous transfer mode (ATM) networking will involve support for the routing and rerouting of active connections. Work published under the auspices of the Telecommunications Industry Association (http://www.tiaonline.org), describes basic architectures and routing protocol issues for satellite ATM (SATATM) networks. The architectures and issues identified will serve as a basis for further development of technical specifications for these SATATM networks. Three ATM network architectures for bent pipe satellites and three ATM network architectures for satellites with onboard ATM switches were developed. The architectures differ from one another in terms of required level of mobility, supported data rates, supported terrestrial interfaces, and onboard processing and switching requirements. The documentation addresses low-, middle-, and geosynchronous-Earth-orbit satellite configurations. The satellite environment may require real-time routing to support the mobility of end devices and nodes of the ATM network itself. This requires the network to be able to reroute active circuits in real time. In addition to supporting mobility, rerouting can also be used to (1) optimize network routing, (2) respond to changing quality-of-service requirements, and (3) provide a fault tolerance mechanism. Traffic management and control functions are necessary in ATM to ensure that the quality-of-service requirements associated with each connection are not violated and also to provide flow and congestion control functions. Functions related to traffic management were identified and described. Most of these traffic management functions will be supported by on-ground ATM switches, but in a hybrid terrestrial-satellite ATM network, some of the traffic management functions may have to be supported by the onboard satellite ATM switch. Future work is planned to examine the tradeoffs of placing traffic management functions onboard a satellite as

Biosensor Architectures for High-Fidelity Reporting of Cellular Signaling

Science.gov (United States)

Dushek, Omer; Lellouch, Annemarie C.; Vaux, David J.; Shahrezaei, Vahid

2014-01-01

Understanding mechanisms of information processing in cellular signaling networks requires quantitative measurements of protein activities in living cells. Biosensors are molecular probes that have been developed to directly track the activity of specific signaling proteins and their use is revolutionizing our understanding of signal transduction. The use of biosensors relies on the assumption that their activity is linearly proportional to the activity of the signaling protein they have been engineered to track. We use mechanistic mathematical models of common biosensor architectures (single-chain FRET-based biosensors), which include both intramolecular and intermolecular reactions, to study the validity of the linearity assumption. As a result of the classic mechanism of zero-order ultrasensitivity, we find that biosensor activity can be highly nonlinear so that small changes in signaling protein activity can give rise to large changes in biosensor activity and vice versa. This nonlinearity is abolished in architectures that favor the formation of biosensor oligomers, but oligomeric biosensors produce complicated FRET states. Based on this finding, we show that high-fidelity reporting is possible when a single-chain intermolecular biosensor is used that cannot undergo intramolecular reactions and is restricted to forming dimers. We provide phase diagrams that compare various trade-offs, including observer effects, which further highlight the utility of biosensor architectures that favor intermolecular over intramolecular binding. We discuss challenges in calibrating and constructing biosensors and highlight the utility of mathematical models in designing novel probes for cellular signaling. PMID:25099816

Employing lidar to detail vegetation canopy architecture for prediction of aeolian transport

Science.gov (United States)

Sankey, Joel B.; Law, Darin J.; Breshears, David D.; Munson, Seth M.; Webb, Robert H.

2013-01-01

The diverse and fundamental effects that aeolian processes have on the biosphere and geosphere are commonly generated by horizontal sediment transport at the land surface. However, predicting horizontal sediment transport depends on vegetation architecture, which is difficult to quantify in a rapid but accurate manner. We demonstrate an approach to measure vegetation canopy architecture at high resolution using lidar along a gradient of dryland sites ranging from 2% to 73% woody plant canopy cover. Lidar-derived canopy height, distance (gaps) between vegetation elements (e.g., trunks, limbs, leaves), and the distribution of gaps scaled by vegetation height were correlated with canopy cover and highlight potentially improved horizontal dust flux estimation than with cover alone. Employing lidar to estimate detailed vegetation canopy architecture offers promise for improved predictions of horizontal sediment transport across heterogeneous plant assemblages.

Aggregate size and architecture determine biomass activity for one-stage partial nitritation and anammox

DEFF Research Database (Denmark)

Vlaeminck, S.; Terada, Akihiko; Smets, Barth F.

2010-01-01

to the inoculation and operation of the reactors. Fluorescent in-situ hybridization (FISH) was applied on aggregate sections to quantify AerAOB and AnAOB, as well as to visualize the aggregate architecture. The activity balance of the aggregates was calculated as the nitrite accumulation rate ratio (NARR), i...... and nitrite sources (NARR, > 1.7). Large A and C aggregates were granules capable of autonomous nitrogen removal (NARR, 0.6 to 1.1) with internal AnAOB zones surrounded by an AerAOB rim. Around 50% of the autotrophic space in these granules consisted of AerAOB- and AnAOB-specific EPS. Large B aggregates were...... thin film-like nitrite sinks (NARR,

HOW DO ARCHITECTS THINK? LEARNING STYLES AND ARCHITECTURAL EDUCATION

Directory of Open Access Journals (Sweden)

Magda Mostafa

2010-07-01

Full Text Available Architecture is a complex process involving the divergent resolution of a multitude of factors- social, ecological, technical, economic, functional, ethical and aesthetic. Despite this diversity all architectural problem solving processes share one common factor- they must be resolved spatially. This paper sets out to explore how best to develop these spatial thinking skills in young architects through addressing their learning styles in education. The primary hypothesis tested is twofold. First using the Solomon & Felder (2007 definition of learning styles and their Index of Learning Styles Questionnaire the average profile of a study group from the freshmen and sophomore architectural student body at the Architectural Engineering Program of the American University in Cairo is mapped and compared to that of a control group from the general population of the university from a cross-section of majors. Secondly, using the Spatial Ability test by Newton & Bristoll (2009, the spatial ability of both the control and study groups are measured and compared. The analysis of these results tests the assumption that the majority of architectural students will be visual, rather than verbal; and active, rather than reflective, learners; as well as exhibiting higher spatial abilities, as compared to the control group. The performance of students in these tests are then correlated against their learning styles profile using the following sets- low spatial ability against both reflective and verbal learning; moderate spatial ability against neutral learning styles; and high spatial ability against both active and visual learning. The results show a particular corroboration between high spatial ability and active learning in the entire group of students- both study, and control- as well as a strong corroboration between high spatial ability and visual learning- with a higher correlation in architecture students, reaching 100% in some classes. It is hoped that by

An architectural model for network interconnection

NARCIS (Netherlands)

van Sinderen, Marten J.; Vissers, C.A.; Kalin, T.

1983-01-01

This paper presents a technique of successive decomposition of a common users' activity to illustrate the problems of network interconnection. The criteria derived from this approach offer a structuring principle which is used to develop an architectural model that embeds heterogeneous subnetworks

Architectural Theatricality

DEFF Research Database (Denmark)

Tvedebrink, Tenna Doktor Olsen

environments and a knowledge gap therefore exists in present hospital designs. Consequently, the purpose of this thesis has been to investigate if any research-based knowledge exist supporting the hypothesis that the interior architectural qualities of eating environments influence patient food intake, health...... and well-being, as well as outline a set of basic design principles ‘predicting’ the future interior architectural qualities of patient eating environments. Methodologically the thesis is based on an explorative study employing an abductive approach and hermeneutic-interpretative strategy utilizing tactics...... and food intake, as well as a series of references exist linking the interior architectural qualities of healthcare environments with the health and wellbeing of patients. On the basis of these findings, the thesis presents the concept of Architectural Theatricality as well as a set of design principles...

Architecture of Institution & Home. Architecture as Cultural Medium

NARCIS (Netherlands)

Robinson, J.W.

2004-01-01

This dissertation addresses how architecture functions as a cultural medium. It does so by by investigating how the architecture of institution and home each construct and support different cultural practices. By studying the design of ordinary settings in terms of how qualitative differences in

Unmasking tandem site interaction in human acetylcholinesterase. Substrate activation with a cationic acetanilide substrate.

Science.gov (United States)

Johnson, Joseph L; Cusack, Bernadette; Davies, Matthew P; Fauq, Abdul; Rosenberry, Terrone L

2003-05-13

Acetylcholinesterase (AChE) contains a narrow and deep active site gorge with two sites of ligand binding, an acylation site (or A-site) at the base of the gorge, and a peripheral site (or P-site) near the gorge entrance. The P-site contributes to catalytic efficiency by transiently binding substrates on their way to the acylation site, where a short-lived acyl enzyme intermediate is produced. A conformational interaction between the A- and P-sites has recently been found to modulate ligand affinities. We now demonstrate that this interaction is of functional importance by showing that the acetylation rate constant of a substrate bound to the A-site is increased by a factor a when a second molecule of substrate binds to the P-site. This demonstration became feasible through the introduction of a new acetanilide substrate analogue of acetylcholine, 3-(acetamido)-N,N,N-trimethylanilinium (ATMA), for which a = 4. This substrate has a low acetylation rate constant and equilibrates with the catalytic site, allowing a tractable algebraic solution to the rate equation for substrate hydrolysis. ATMA affinities for the A- and P-sites deduced from the kinetic analysis were confirmed by fluorescence titration with thioflavin T as a reporter ligand. Values of a >1 give rise to a hydrolysis profile called substrate activation, and the AChE site-specific mutant W86F, and to a lesser extent wild-type human AChE itself, showed substrate activation with acetylthiocholine as the substrate. Substrate activation was incorporated into a previous catalytic scheme for AChE in which a bound P-site ligand can also block product dissociation from the A-site, and two additional features of the AChE catalytic pathway were revealed. First, the ability of a bound P-site ligand to increase the substrate acetylation rate constant varied with the structure of the ligand: thioflavin T accelerated ATMA acetylation by a factor a(2) of 1.3, while propidium failed to accelerate. Second, catalytic rate

On Detailing in Contemporary Architecture

DEFF Research Database (Denmark)

Kristensen, Claus; Kirkegaard, Poul Henning

2010-01-01

Details in architecture have a significant influence on how architecture is experienced. One can touch the materials and analyse the detailing - thus details give valuable information about the architectural scheme as a whole. The absence of perceptual stimulation like details and materiality...... / tactility can blur the meaning of the architecture and turn it into an empty statement. The present paper will outline detailing in contemporary architecture and discuss the issue with respect to architectural quality. Architectural cases considered as sublime piece of architecture will be presented...

Mesoporous titanium dioxide (TiO2) with hierarchically 3D dendrimeric architectures: formation mechanism and highly enhanced photocatalytic activity.

Science.gov (United States)

Li, Xiao-Yun; Chen, Li-Hua; Rooke, Joanna Claire; Deng, Zhao; Hu, Zhi-Yi; Wang, Shao-Zhuan; Wang, Li; Li, Yu; Krief, Alain; Su, Bao-Lian

2013-03-15

Mesoporous TiO(2) with a hierarchically 3D dendrimeric nanostructure comprised of nanoribbon building units has been synthesized via a spontaneous self-formation process from various titanium alkoxides. These hierarchically 3D dendrimeric architectures can be obtained by a very facile, template-free method, by simply dropping a titanium butoxide precursor into methanol solution. The novel configuration of the mesoporous TiO(2) nanostructure in nanoribbon building units yields a high surface area. The calcined samples show significantly enhanced photocatalytic activity and degradation rates owing to the mesoporosity and their improved crystallinity after calcination. Furthermore, the 3D dendrimeric architectures can be preserved after phase transformation from amorphous TiO(2) to anatase or rutile, which occurs during calcination. In addition, the spontaneous self-formation process of mesoporous TiO(2) with hierarchically 3D dendrimeric architectures from the hydrolysis and condensation reaction of titanium butoxide in methanol has been followed by in situ optical microscopy (OM), revealing the secret on the formation of hierarchically 3D dendrimeric nanostructures. Moreover, mesoporous TiO(2) nanostructures with similar hierarchically 3D dendrimeric architectures can also be obtained using other titanium alkoxides. The porosities and nanostructures of the resultant products were characterized by SEM, TEM, XRD, and N(2) adsorption-desorption measurements. The present work provides a facile and reproducible method for the synthesis of novel mesoporous TiO(2) nanoarchitectures, which in turn could herald the fabrication of more efficient photocatalysts. Copyright © 2012 Elsevier Inc. All rights reserved.

Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals

Directory of Open Access Journals (Sweden)

Elela Sherif

2006-01-01

Full Text Available Abstract Background We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing because they contain a portion complementary to sequences immediately upstream of the target 5' splice site combined with a non-hybridizing 5' tail carrying binding sites for the hnRNP A1/A2 proteins. In the present study, we have tested the inhibitory activity of RNA oligonucleotides carrying different tail structures. Results We show that an oligonucleotide with a 5' tail containing the human β-globin branch site sequence inhibits the use of the 5' splice site of Bcl-xL, albeit less efficiently than a tail containing binding sites for the hnRNP A1/A2 proteins. A branch site-containing tail positioned at the 3' end of the oligonucleotide also elicited splicing inhibition but not as efficiently as a 5' tail. The interfering activity of a 3' tail was improved by adding a 5' splice site sequence next to the branch site sequence. A 3' tail carrying a Y-shaped branch structure promoted similar splicing interference. The inclusion of branch site or 5' splice site sequences in the Y-shaped 3' tail further improved splicing inhibition. Conclusion Our in vitro results indicate that a variety of tail architectures can be used to elicit splicing interference at low nanomolar concentrations, thereby broadening the scope and the potential impact of this antisense technology.

Systemic Architecture

DEFF Research Database (Denmark)

Poletto, Marco; Pasquero, Claudia

-up or tactical design, behavioural space and the boundary of the natural and the artificial realms within the city and architecture. A new kind of "real-time world-city" is illustrated in the form of an operational design manual for the assemblage of proto-architectures, the incubation of proto-gardens...... and the coding of proto-interfaces. These prototypes of machinic architecture materialize as synthetic hybrids embedded with biological life (proto-gardens), computational power, behavioural responsiveness (cyber-gardens), spatial articulation (coMachines and fibrous structures), remote sensing (FUNclouds...

Research of Ancient Architectures in Jin-Fen Area Based on GIS and BIM Technology

International Nuclear Information System (INIS)

Jia, Jing; Zheng, Qiuhong; Gao, Huiying; Sun, Hai

2017-01-01

The number of well-preserved ancient buildings located in Shanxi Province, enjoying the absolute maximum proportion of ancient architectures in China, is about 18418, among which, 9053 buildings have the structural style of wood frame. The value of the application of BIM (Building Information Modeling) and GIS (Geographic Information System) is gradually probed and testified in the corresponding fields of ancient architecture’s spatial distribution information management, routine maintenance and special conservation and restoration, the evaluation and simulation of related disasters, such as earthquake. The research objects are ancient architectures in JIN-FEN area, which were first investigated by Sicheng LIANG and recorded in his work of “Chinese ancient architectures survey report”. The research objects, i.e. the ancient architectures in Jin-Fen area include those in Sicheng LIANG’s investigation, and further adjustments were made through authors’ on-site investigation and literature searching and collection. During this research process, the spatial distributing Geodatabase of research objects is established utilizing GIS. The BIM components library for ancient buildings is formed combining on-site investigation data and precedent classic works, such as “Yingzao Fashi”, a treatise on architectural methods in Song Dynasty, “Yongle Encyclopedia” and “Gongcheng Zuofa Zeli”, case collections of engineering practice, by the Ministry of Construction of Qing Dynasty. A building of Guangsheng temple in Hongtong county is selected as an example to elaborate the BIM model construction process based on the BIM components library for ancient buildings. Based on the foregoing work results of spatial distribution data, attribute data of features, 3D graphic information and parametric building information model, the information management system for ancient architectures in Jin-Fen Area, utilizing GIS and BIM technology, could be constructed to support

Effluent Storage and Biomat Occurrence among Septic System Absorption Field Architectures in a Typic Fragiudult.

Science.gov (United States)

Prater, N J M; Brye, K R; Dunn, S; Soerens, T S; Sharpley, A N; Mason, E; Gbur, E E

2013-07-01

On-site wastewater treatment systems (OWTSs) are commonly used by households in areas of low population density to treat household wastewater and recycle it back to the environment. However, new absorption field products of differing architecture types have recently become available. A 3-yr field study was conducted in Bethel Heights, northwest Arkansas to assess several newer architecture types (i.e., chambers, polystyrene-aggregate, and gravel-less pipe) relative to the traditional pipe-and-gravel design under wet- and dry-soil conditions. Thirteen products of four different architecture types were installed in 46-cm-deep trenches in a Captina silt loam (fine-silty, siliceous, active, mesic Typic Fragiudult). Products were evaluated based on in-trench solution storage measured with an electronic water-level sensor approximately weekly from January 2009 through January 2012. Between May 2010 and January 2012, the thickness of any biomat formation was measured approximately weekly by insertion of a wooden dowel through in-trench monitoring ports. Architecture type alone did not affect ( > 0.05) in-trench solution storage. However, solution storage among individual products differed under wet- and dry-soil conditions ( storage but that several alternative products also had greater solution storage than that of the traditional pipe-and-gravel system. With no observed effluent surfacing, the soil morphology approach appears to be adequate and appropriately environmentally conservative for assigning typical single-family loading rates to alternative OWTS products and to the traditional pipe-and-gravel system. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

Digitally-Driven Architecture

Directory of Open Access Journals (Sweden)

Henriette Bier

2010-06-01

Full Text Available The shift from mechanical to digital forces architects to reposition themselves: Architects generate digital information, which can be used not only in designing and fabricating building components but also in embedding behaviours into buildings. This implies that, similar to the way that industrial design and fabrication with its concepts of standardisation and serial production influenced modernist architecture, digital design and fabrication influences contemporary architecture. While standardisa­tion focused on processes of rationalisation of form, mass-customisation as a new paradigm that replaces mass-production, addresses non-standard, complex, and flexible designs. Furthermore, knowledge about the designed object can be encoded in digital data pertaining not just to the geometry of a design but also to its physical or other behaviours within an environment. Digitally-driven architecture implies, therefore, not only digitally-designed and fabricated architecture, it also implies architecture – built form – that can be controlled, actuated, and animated by digital means. In this context, this sixth Footprint issue examines the influence of digital means as prag­matic and conceptual instruments for actuating architecture. The focus is not so much on computer-based systems for the development of architectural designs, but on architecture incorporating digital control, sens­ing, actuating, or other mechanisms that enable buildings to inter­act with their users and surroundings in real time in the real world through physical or sensory change and variation.

Hydrogenation active sites of unsupported molybdenum sulfide catalysts for hydroprocessing heavy oils

Energy Technology Data Exchange (ETDEWEB)

Iwata, Y.; Araki, Y.; Honna, K. [Tsukuba-branch, Advanced Catalyst Research Laboratory, Petroleum Energy Center, 1-1 Higashi, Tsukuba, 305-8565 Ibaraki (Japan); Miki, Y.; Sato, K.; Shimada, H. [National Institute of Materials and Chemical Research, 1-1 Higashi, Tsukuba, 305-8565 Ibaraki (Japan)

2001-02-20

The purpose of the present study was to elucidate the nature of the hydrogenation active sites on unsupported molybdenum sulfide catalysts, aimed at the improvement of the catalysts for the slurry processes. The number of hydrogenation active sites was found to relate to the 'inflection' on the basal plane of the catalyst particles. The comparison of the catalytic activity to that of an oil-soluble catalyst in the hydroprocessing of heavy oils suggests that the performance of the oil-soluble catalyst was near the maximum, unless another component such as Ni or Co was incorporated.

On methods of sustainable architectural design of bio-positive buildings in the low-rise residential development structure

Directory of Open Access Journals (Sweden)

Zhogoleva Anna

2017-01-01

Full Text Available The purpose of the author’s research is to determine the actual content of sustainable architectural design for suburban residential development. In accordance with the methodology of area sustainable development the traditional architectural design according to the rules and regulations is completed with additional approaches and methods. As a result, methods of bio-positive design of buildings have been studied and defined, including: the principle of planning transformations, the use of environmentally friendly, local building materials and design concepts, energy-efficient architectural design, the use of alternative energy in building operation, the design of the energy intake and accumulationsystems, the architectural and landscape design that ensures stable functioning of autonomous, sustainable biosystems on the site, non-waste functioning of architectural objects, introduction of waste disposal systems in the project.

Quality Assurance in Architectural Education in Asia – On the Perspective of ‘Design’ Based Architectural Education and its Holistic Assessment

Directory of Open Access Journals (Sweden)

Lee Junsuk

2018-01-01

eventually evolve to become uniform without a character. At the same time, it must possess enough specificity to guide panel reviewers in judging competency level of the student learning at the actual site. As a conclusion, there is paramount importance in architecture education. It is the UNESCO/UIA Charter that asks all civilized architectural education to be self-assessed and peerreviewed for continuing improvement. Considering current era, there is no doubt that there are many opportunities waiting ahead among us. Familiarizing and building sympathy to the voice of the ‘Charter for Architectural Education.’ will be the most proper place to start.

Architecture and Stages

DEFF Research Database (Denmark)

Kiib, Hans

2009-01-01

as "experiencescape" - a space between tourism, culture, learning and economy. Strategies related to these challenges involve new architectural concepts and art as ‘engines' for a change. New expressive architecture and old industrial buildings are often combined into hybrid narratives, linking the past...... with the future. But this is not enough. The agenda is to develop architectural spaces, where social interaction and learning are enhanced by art and fun. How can we develop new architectural designs in our inner cities and waterfronts where eventscapes, learning labs and temporal use are merged with everyday...

Architecture in Its Own Shadow

Directory of Open Access Journals (Sweden)

Alexander Rappaport

2016-11-01

Full Text Available Those who consider themselves architects disapprove of the statements about destruction of the subject of architectural culture, profession and of the subject of architectural theory. At the same time, a deep crisis of both theory and practice is obvious. When theorists of architecture of the 20th and early 21st centuries turned to the subjects external to architecture – sociology, psychology, semiotics, ecology, post-structuralist criticism, etc., instead of enriching and renovating the architectural theory, the results were just the opposite. A brand new and independent paradigm of architecture is needed. It should contain three parts specific by their logical-subject nature: ontology of architecture, methodology of architectural thought and axiology of architectural thought.

Humanizing Architecture

DEFF Research Database (Denmark)

Toft, Tanya Søndergaard

2015-01-01

The article proposes the urban digital gallery as an opportunity to explore the relationship between ‘human’ and ‘technology,’ through the programming of media architecture. It takes a curatorial perspective when proposing an ontological shift from considering media facades as visual spectacles...... agency and a sense of being by way of dematerializing architecture. This is achieved by way of programming the symbolic to provide new emotional realizations and situations of enlightenment in the public audience. This reflects a greater potential to humanize the digital in media architecture....

Robotic architectures

CSIR Research Space (South Africa)

Mtshali, M

2010-01-01

Full Text Available In the development of mobile robotic systems, a robotic architecture plays a crucial role in interconnecting all the sub-systems and controlling the system. The design of robotic architectures for mobile autonomous robots is a challenging...

Report on functional requirements and software architecture for the IDTO prototype : phase I demonstration site (Columbus).

Science.gov (United States)

2013-08-01

This report documents the System Requirements and Architecture for the Phase I implementation of the Integrated Dynamic : Transit Operations (IDTO) Prototype bundle within the Dynamic Mobility Applications (DMA) portion of the Connected Vehicle : Pro...

Synthesis of Bi2O3 architectures in DMF–H2O solution by precipitation method and their photocatalytic activity

International Nuclear Information System (INIS)

Yang, Li-Li; Han, Qiao-Feng; Zhao, Jin; Zhu, Jun-Wu; Wang, Xin; Ma, Wei-Hua

2014-01-01

Graphical abstract: Flowerlike α-Bi 2 O 3 architectures assembled by nanobrick-based petals with pineapple surface were firstly synthesized by precipitation method at room temperature in DMF–H 2 O solution. - Highlights: • Nanobrick-based flowerlike Bi 2 O 3 crystals with pineapple surface were synthesized by precipitation method. • Good solubility of Bi(NO 3 ) 3 in DMF played a crucial role in the growth of flowerlike Bi 2 O 3 . • The growth mechanism of Bi 2 O 3 microcrystallites has been explained in detail. - Abstract: Well-crystalline flowerlike α-Bi 2 O 3 hierarchical architectures with pineapple-shaped petals have been synthesized by precipitation method at a volume ratio of DMF/H 2 O of 5, where DMF and H 2 O were used to dissolve Bi(NO 3 ) 3 and KOH, respectively. If the DMF/H 2 O ratio was decreased to 2:1, 1:1 and 0:30, flower-, bundle- and dendrite-shaped α-Bi 2 O 3 microcrystallites aggregated by nanorods were formed, respectively. The simple synthetic route and thus obtained Bi 2 O 3 architectures of various morphologies provide a basis insight for their formation mechanism. The photocatalytic activity of the as-prepared Bi 2 O 3 particles for degradation of Rhodamine B (RhB) under visible-light irradiation was obviously influenced by their morphologies. Bi 2 O 3 of nanorod-based microstructures exhibited higher photodegradation activity than nanobrick-based ones, owing to higher light absorption and carrier separation efficiency in one-dimensional (1D) nanostructured materials

Combining Silviculture and Landscape Architecture to Enhance the Roadside View

Science.gov (United States)

Philip M. McDonald; R. Burton Litton Jr.

1998-01-01

On a high-quality site in the mixed conifer forest of northern California, understory and overstory vegetation along a 3-mile paved county road were manipulated to enhance the view for the traveler. Traditional silvicultural cutting methods and landscape architectural techniques were blended to give contrast and variability to the vegetation along both sides of the...

Investigation of Transformer Winding Architectures for High Voltage Capacitor Charging Applications

DEFF Research Database (Denmark)

Schneider, Henrik; Thummala, Prasanth; Huang, Lina

2014-01-01

Transformer parameters such as leakage inductance and self-capacitance are rarely calculated in advance during the design phase, because of the complexity and huge analytical error margins caused by practical winding implementation issues. Thus, choosing one transformer architecture over another ...... converter used to drive a dielectric electro active polymer based incremental actuator. The total losses due to the transformer parasitics for the best transformer architectures is reduced by more than a factor of ten compared to the worst case transformer architectures....

Architecture Sustainability

NARCIS (Netherlands)

Avgeriou, Paris; Stal, Michael; Hilliard, Rich

2013-01-01

Software architecture is the foundation of software system development, encompassing a system's architects' and stakeholders' strategic decisions. A special issue of IEEE Software is intended to raise awareness of architecture sustainability issues and increase interest and work in the area. The

A New Electronic Commerce Architecture in the Cloud

OpenAIRE

Guigang Zhang; Chao Li; Sixin Xue; Yuenan Liu; Yong Zhang; Chunxiao Xing

2012-01-01

In this paper, the authors propose a new electronic commerce architecture in the cloud that satisfies the requirements of the cloud. This architecture includes five technologies, which are the massive EC data storage technology in the cloud, the massive EC data processing technology in the cloud, the EC security management technology in the cloud, OLAP technology for EC in the cloud, and active EC technology in the cloud. Finally, a detailed discussion of future trends for EC in the cloud env...

In Search of the Organic: An Unequivocal Exploration into the Principles of Organic Architecture

OpenAIRE

Duncan, Matthew Alexander

2016-01-01

The architecture is a multi-venue performing arts complex consisting of a full-scale opera house, a mid-size open-air theater, a small recital salon, and an outdoor arena-style street-stage. The building is sited at Buzzard Point in Washington, DC at the terminus of South Capitol Street along the Anacostia River. The thesis is an exploration into the concept of organic architecture and a formal study of its application to the order, structure, and construction of a building. MARCH

Orthogonal use of a human tRNA synthetase active site to achieve multi-functionality

Science.gov (United States)

Zhou, Quansheng; Kapoor, Mili; Guo, Min; Belani, Rajesh; Xu, Xiaoling; Kiosses, William B.; Hanan, Melanie; Park, Chulho; Armour, Eva; Do, Minh-Ha; Nangle, Leslie A.; Schimmel, Paul; Yang, Xiang-Lei

2011-01-01

Protein multi-functionality is an emerging explanation for the complexity of higher organisms. In this regard, while aminoacyl tRNA synthetases catalyze amino acid activation for protein synthesis, some also act in pathways for inflammation, angiogenesis, and apoptosis. How multiple functions evolved and their relationship to the active site is not clear. Here structural modeling analysis, mutagenesis, and cell-based functional studies show that the potent angiostatic, natural fragment of human TrpRS associates via Trp side chains that protrude from the cognate cellular receptor VE-cadherin. Modeling indicates that (I prefer the way it was because the conclusion was reached not only by modeling, but more so by experimental studies.)VE-cadherin Trp side chains fit into the Trp-specific active site of the synthetase. Thus, specific side chains of the receptor mimic (?) amino acid substrates and expand the functionality of the active site of the synthetase. We propose that orthogonal use of the same active site may be a general way to develop multi-functionality of human tRNA synthetases and other proteins. PMID:20010843

Advanced and secure architectural EHR approaches.

Science.gov (United States)

Blobel, Bernd

2006-01-01

, the Australian GEHR project, the openEHR initiative, the revision of CEN ENV 13606 "Electronic Health Record communication", all based on Archetypes, but also the HL7 version 3 activities are discussed in some detail. The latter include the HL7 RIM, the HL7 Development Framework, the HL7's clinical document architecture (CDA) as well as the set of models from use cases, activity diagrams, sequence diagrams up to Domain Information Models (DMIMs) and their building blocks Common Message Element Types (CMET) Constraining Models to their underlying concepts. The future-proof EHR architecture as open, user-centric, user-friendly, flexible, scalable, portable core application in health information systems and health networks has to follow advanced architectural paradigms.

A Tale of Two Imperial Residences: Aurangzeb’s Architectural Patronage

Directory of Open Access Journals (Sweden)

Pushkar Sohoni

2016-12-01

Full Text Available While the Mughal Emperor Aurangzeb Alamgir is not well-known for his architectural commissions as his predecessors, there are still a few extant buildings associated with him. Two of these are residential sites, built almost fifty years apart, at the beginning and end of his career as an emperor. This essay examines these hitherto unpublished sites, and documents them as part of a Mughal legacy in the Deccan that has remained uncelebrated. The palace at Aurangabad and a royal residential compound just outside of Ahmadnagar are the two sites which bracket the emperor’s career and offer an insight into his political fortunes and religious life as it changed.

Active site of tripeptidyl peptidase II from human erythrocytes is of the subtilisin type

Energy Technology Data Exchange (ETDEWEB)

Tomkinson, B.; Wernstedt, C.; Hellman, U.; Zetterqvist, Oe.

1987-11-01

The present report presents evidence that the amino acid sequence around the serine of the active site of human tripeptidyl peptidase II is of the subtilisin type. The enzyme from human erythrocytes was covalently labeled at its active site with (/sup 3/H)diisopropyl fluorophosphate, and the protein was subsequently reduced, alkylated, and digested with trypsin. The labeled tryptic peptides were purified by gel filtration and repeated reversed-phase HPLC, and their amino-terminal sequences were determined. Residue 9 contained the radioactive label and was, therefore, considered to be the active serine residue. The primary structure of the part of the active site (residues 1-10) containing this residue was concluded to be Xaa-Thr-Gln-Leu-Met-Asx-Gly-Thr-Ser-Met. This amino acid sequence is homologous to the sequence surrounding the active serine of the microbial peptidases subtilisin and thermitase. These data demonstrate that human tripeptidyl peptidase II represents a potentially distinct class of human peptidases and raise the question of an evolutionary relationship between the active site of a mammalian peptidase and that of the subtilisin family of serine peptidases.

High-level language computer architecture

CERN Document Server

Chu, Yaohan

1975-01-01

High-Level Language Computer Architecture offers a tutorial on high-level language computer architecture, including von Neumann architecture and syntax-oriented architecture as well as direct and indirect execution architecture. Design concepts of Japanese-language data processing systems are discussed, along with the architecture of stack machines and the SYMBOL computer system. The conceptual design of a direct high-level language processor is also described.Comprised of seven chapters, this book first presents a classification of high-level language computer architecture according to the pr

Poisoning Experiments Aimed at Discriminating Active and Less-Active Sites of Silica-Supported Tantalum Hydride for Alkane Metathesis

KAUST Repository

Saggio, Guillaume; Taoufik, Mostafa; Basset, Jean-Marie; Thivolle-Cazat, Jean

2010-01-01

Only 50% of the silica-supported tantalum hydride sites are active in the metathesis of propane. Indeed, more than 45% of the tantalum hydride can be eliminated by a selective oxygen poisoning of inactive sites with no significant decrease

Grid Architecture 2

Energy Technology Data Exchange (ETDEWEB)

Taft, Jeffrey D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

2016-01-01

The report describes work done on Grid Architecture under the auspices of the Department of Electricity Office of Electricity Delivery and Reliability in 2015. As described in the first Grid Architecture report, the primary purpose of this work is to provide stakeholder insight about grid issues so as to enable superior decision making on their part. Doing this requires the creation of various work products, including oft-times complex diagrams, analyses, and explanations. This report provides architectural insights into several important grid topics and also describes work done to advance the science of Grid Architecture as well.

Information Architecture: The Data Warehouse Foundation.

Science.gov (United States)

Thomas, Charles R.

1997-01-01

Colleges and universities are initiating data warehouse projects to provide integrated information for planning and reporting purposes. A survey of 40 institutions with active data warehouse projects reveals the kinds of tools, contents, data cycles, and access currently used. Essential elements of an integrated information architecture are…

Leadership in Architectural Research: Between Academia and the Profession

Directory of Open Access Journals (Sweden)

Hazem Rashed-Ali

2012-10-01

Full Text Available Recent decades have witnessed a notable expansion of architectural research activities, with respect to both subject and methodology. This expansion can be mostly credited to an increase in government and private funding of primarily academic research initiatives. More recently, however, a noticeable increase in research activities within the architectural profession makes it possible to argue that it is the profession itself that is now taking leadership in the development of contemporary research agendas. This growing significance of architectural research, in both academia and the profession,is ultimately a response to the diverse challenges facing the profession; most notably, the issue of environmental sustainability, but also including the rapid pace of technological change, the increase ddiversity of users, and the growing complexity of architectural projects. Engaging research is an essential factor in facing these challenges as well as taking full advantage of the opportunities they offer. For this research to be most effective, however, a greater perspective and a clearer definition of its role and the goals it can aspire to, in both academia and the profession, are needed; and most importantly, the question becomes, how do we foster a more integrated research culture between academia and the profession?

Democratic management and architecture school

Directory of Open Access Journals (Sweden)

Silvana Aparecida de Souza

2011-10-01

Full Text Available It is a conceptual and theoretical research on school organization and its democratization, focusing on one aspect of an objective nature: its architecture. The study was based on the academic literature on democratization and theoretical contribution of Michel Foucault, with regard to the analysis of space as a resourcecontrol, surveillance and training, going through a historical review of the modelconstruction of school buildings in Brazil. It is therefore a sociological analysis of the school environment, in relation to the democratization process of basic education, understood as ensuring that the conditions of access and permanence to a universalquality education, and conceived and gestated from collective interests of its users.We conclude that the architecture of public schools in Brazil do not provides democratic management, either by format controller of buildings constructed in the republican period, either by the current economic priority for the construction of public school buildings, which includes little or no space for collective activities. The character of the buildings remains controller, no more for its architecture, but made possible by technological development, which allows monitoring by video cameras, which is made with the permission and support of community.

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents.

Science.gov (United States)

Stepankova, Veronika; Khabiri, Morteza; Brezovsky, Jan; Pavelka, Antonin; Sykora, Jan; Amaro, Mariana; Minofar, Babak; Prokop, Zbynek; Hof, Martin; Ettrich, Rudiger; Chaloupkova, Radka; Damborsky, Jiri

2013-05-10

The use of enzymes for biocatalysis can be significantly enhanced by using organic cosolvents in the reaction mixtures. Selection of the cosolvent type and concentration range for an enzymatic reaction is challenging and requires extensive empirical testing. An understanding of protein-solvent interaction could provide a theoretical framework for rationalising the selection process. Here, the behaviour of three model enzymes (haloalkane dehalogenases) was investigated in the presence of three representative organic cosolvents (acetone, formamide, and isopropanol). Steady-state kinetics assays, molecular dynamics simulations, and time-resolved fluorescence spectroscopy were used to elucidate the molecular mechanisms of enzyme-solvent interactions. Cosolvent molecules entered the enzymes' access tunnels and active sites, enlarged their volumes with no change in overall protein structure, but surprisingly did not act as competitive inhibitors. At low concentrations, the cosolvents either enhanced catalysis by lowering K(0.5) and increasing k(cat), or caused enzyme inactivation by promoting substrate inhibition and decreasing k(cat). The induced activation and inhibition of the enzymes correlated with expansion of the active-site pockets and their occupancy by cosolvent molecules. The study demonstrates that quantitative analysis of the proportions of the access tunnels and active-sites occupied by organic solvent molecules provides the valuable information for rational selection of appropriate protein-solvent pair and effective cosolvent concentration. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Towards a semantic web layered architecture

CSIR Research Space (South Africa)

Gerber, AJ

2007-02-01

Full Text Available as an architectural pattern or architectural style [6, 43]. In this section we give a brief description of the con- cepts software architecture and layered architecture. In ad- dition we provide a summary of a list of criteria for layered architectures identified...- els caused some architectural recurrences to evolve. These are described as architectural patterns [6] or architectural styles [43]. Examples of the best known architectural patterns include, but are not limited to, the client/server architectural...

Threatened and endangered wildlife species of the Hanford Site related to CERCLA characterization activities

Energy Technology Data Exchange (ETDEWEB)

Fitzner, R.E. [Pacific Northwest Lab., Richland, WA (United States); Weiss, S.G.; Stegen, J.A. [Westinghouse Hanford Co., Richland, WA (United States)

1994-06-01

The US Department of Energy`s (DOE) Hanford Site has been placed on the National Priorities List, which requires that it be remediated under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) or Superfund. Potentially contaminated areas of the Hanford Site were grouped into operable units, and detailed characterization and investigation plans were formulated. The DOE Richland Operations Office requested Westinghouse Hanford Company (WHC) to conduct a biological assessment of the potential impact of these characterization activities on the threatened, endangered, and sensitive wildlife species of the Hanford Site. Additional direction for WHC compliances with wildlife protection can be found in the Environmental Compliance Manual. This document is intended to meet these requirements, in part, for the CERCLA characterization activities, as well as for other work comparable in scope. This report documents the biological assessment and describes the pertinent components of the Hanford Site as well as the planned characterization activities. Also provided are accounts of endangered, threatened, and federal candidate wildlife species on the Hanford Site and information as to how human disturbances can affect these species. Potential effects of the characterization activities are described with recommendations for mitigation measures.

Information Integration Architecture Development

OpenAIRE

Faulkner, Stéphane; Kolp, Manuel; Nguyen, Duy Thai; Coyette, Adrien; Do, Thanh Tung; 16th International Conference on Software Engineering and Knowledge Engineering

2004-01-01

Multi-Agent Systems (MAS) architectures are gaining popularity for building open, distributed, and evolving software required by systems such as information integration applications. Unfortunately, despite considerable work in software architecture during the last decade, few research efforts have aimed at truly defining patterns and languages for designing such multiagent architectures. We propose a modern approach based on organizational structures and architectural description lan...

A simplified method for active-site titration of lipases immobilised on hydrophobic supports.

Science.gov (United States)

Nalder, Tim D; Kurtovic, Ivan; Barrow, Colin J; Marshall, Susan N

2018-06-01

The aim of this work was to develop a simple and accurate protocol to measure the functional active site concentration of lipases immobilised on highly hydrophobic supports. We used the potent lipase inhibitor methyl 4-methylumbelliferyl hexylphosphonate to titrate the active sites of Candida rugosa lipase (CrL) bound to three highly hydrophobic supports: octadecyl methacrylate (C18), divinylbenzene crosslinked methacrylate (DVB) and styrene. The method uses correction curves to take into account the binding of the fluorophore (4-methylumbelliferone, 4-MU) by the support materials. We showed that the uptake of the detection agent by the three supports is not linear relative to the weight of the resin, and that the uptake occurs in an equilibrium that is independent of the total fluorophore concentration. Furthermore, the percentage of bound fluorophore varied among the supports, with 50 mg of C18 and styrene resins binding approximately 64 and 94%, respectively. When the uptake of 4-MU was calculated and corrected for, the total 4-MU released via inhibition (i.e. the concentration of functional lipase active sites) could be determined via a linear relationship between immobilised lipase weight and total inhibition. It was found that the functional active site concentration of immobilised CrL varied greatly among different hydrophobic supports, with 56% for C18, compared with 14% for DVB. The described method is a simple and robust approach to measuring functional active site concentration in immobilised lipase samples. Copyright © 2018 Elsevier Inc. All rights reserved.

Characterization and sequencing of the active site of 1-aminocyclopropane-1-carboxylate synthase

International Nuclear Information System (INIS)

Yip, Wing-Kin; Dong, Jian-Guo; Yang, S.F.; Kenny, J.W.; Thompson, G.A.

1990-01-01

The pyridoxal phosphate (PLP)-dependent 1-aminocyclopropane-1-carboxylic acid (ACC) synthase the key enzyme in ethylene biosynthesis, is inactivated by its substrate S-adenosylmethionine (AdoMet). Apple ACC synthase was purified with an immunoaffinity gel, and its active site was probed with NaB 3 H 4 or Ado[ 14 C]Met. Peptide sequencing of both 3 H- and 14 C-labeled peptides revealed a common dodecapeptide of Ser-Leu-Ser-Xaa-Asp-Leu-Gly-Leu-Pro-Gly-Phe-Arg, where Xaa was the modified, radioactive residue in each case. Acid hydrolysis of the 3 H-labeled enzyme released radioactive N-pyridoxyllysine, indicating that the active-site peptide contained lysine at position 4. Mass spectrometry of the 14 C-labeled peptide indicated a protonated molecular ion at m/z 1390.6, from which the mass of Xaa was calculated to be 229, a number that is equivalent to the mass of a lysine residue alkylated by the 2-aminobutyrate portion of AdoMet, as we previously proposed. These results indicate that the same active-site lysine binds the PLP and convalently links to the 2-aminobutyrate portion of AdoMet during inactivation. The active site of tomato ACC synthase was probed in the same manner with Ado [ 14 C]Met. Sequencing of the tomato active-site peptide revealed two highly conserved dodecapeptides; the minor peptide possessed a sequence identical to that of the apple enzyme, whereas the major peptide differed from the minor peptide in that methionine replaced leucine at position 6

Decommissioning and decontamination activity, Gnome Site, Eddy County, New Mexico

International Nuclear Information System (INIS)

1978-10-01

The purpose of this assessment is to present a brief description of the proposed activity and its potential impacts on the environment. This assessment will constitute an evaluation as to whether or not a formal Environmental Statement need be prepared. As background to the proposed activity, Project Gnome was an underground nuclear test conducted in December 1961 as part of the PLOWSHARE Program. The project site is located about 25 miles southeast of Carlsbad, New Mexico. By means of an excavated shaft and tunnel, a 3-kiloton nuclear explosive was emplaced and detonated in a salt bed about 1200 feet below the surface. The uncontaminated rock and salt muck from the original excavation and subsequent contaminated muck and minor construction debris from reentry activities into the nuclear cavity is commingled and stored in a pile near the Gnome/Coach Shaft. Other areas on the site are known to have been contaminated. In 1969, a program was conducted to cleanup and dispose of all surface contamination to whatever depth it occurred in excess of 0.1 mR/hr. Contaminated materials and soil were collected and disposed into the Gnome shaft, which was filled and sealed. Since then, NV has proposed to DOE/HQ much lower criteria for residual radioactive contamination for the Gnome Site. These proposed criteria were to collect and dispose of surficial materials which contain more than 2 x 10 -5 microcuries per gram of soil for beta/gamma emitters and 3 x 10 -2 microcuries per milliliter of tritium in soil moisture. According to the latest reconnaissance in 1972, low concentrations of Cs-137, Sr-90 and tritium were present at various locations on the site in excess of these proposed guidelines. Other operational areas within the site are suspected of containing radioactive contamination in much lesser volume, which are to be determined by careful probing and monitoring, as described in the next section

Architectural Contestation

NARCIS (Netherlands)

Merle, J.

2012-01-01

This dissertation addresses the reductive reading of Georges Bataille's work done within the field of architectural criticism and theory which tends to set aside the fundamental ‘broken’ totality of Bataille's oeuvre and also to narrowly interpret it as a mere critique of architectural form,

Urban landscape and architectural workshop Škocjan Caves 2007

Directory of Open Access Journals (Sweden)

Andrej Mahovič

2012-01-01

Full Text Available The fundamental aim and purpose of the Škocjan Caves 2007 student workshop was for students participating in the seminar of the Faculty of Architecture in Ljubljana to recognise and address some specific spatial and design issues for the further sustainable development of the Škocjan Caves. The working hypothesis of the workshop was that, in spite of significant results in the protection and development of the Caves which have been achieved in the past ten years, it is still necessary to address some spatial issues through an academic process, and to offer different architectural and spatial solutions. The aforesaid is even more important as the Caves are recognised as the only natural and cultural heritage of the Republic of Slovenia to be listed as a UNESCO World Heritage site since 1986.

Connecting Requirements to Architecture and Analysis via Model-Based Systems Engineering

Science.gov (United States)

Cole, Bjorn F.; Jenkins, J. Steven

2015-01-01

In traditional systems engineering practice, architecture, concept development, and requirements development are related but still separate activities. Concepts for operation, key technical approaches, and related proofs of concept are developed. These inform the formulation of an architecture at multiple levels, starting with the overall system composition and functionality and progressing into more detail. As this formulation is done, a parallel activity develops a set of English statements that constrain solutions. These requirements are often called "shall statements" since they are formulated to use "shall." The separation of requirements from design is exacerbated by well-meaning tools like the Dynamic Object-Oriented Requirements System (DOORS) that remained separated from engineering design tools. With the Europa Clipper project, efforts are being taken to change the requirements development approach from a separate activity to one intimately embedded in formulation effort. This paper presents a modeling approach and related tooling to generate English requirement statements from constraints embedded in architecture definition.

Safety-Critical Partitioned Software Architecture: A Partitioned Software Architecture for Robotic

Science.gov (United States)

Horvath, Greg; Chung, Seung H.; Cilloniz-Bicchi, Ferner

2011-01-01

The flight software on virtually every mission currently managed by JPL has several major flaws that make it vulnerable to potentially fatal software defects. Many of these problems can be addressed by recently developed partitioned operating systems (OS). JPL has avoided adopting a partitioned operating system on its flight missions, primarily because doing so would require significant changes in flight software design, and the risks associated with changes of that magnitude cannot be accepted by an active flight project. The choice of a partitioned OS can have a dramatic effect on the overall system and software architecture, allowing for realization of benefits far beyond the concerns typically associated with the choice of OS. Specifically, we believe that a partitioned operating system, when coupled with an appropriate architecture, can provide a strong infrastructure for developing systems for which reusability, modifiability, testability, and reliability are essential qualities. By adopting a partitioned OS, projects can gain benefits throughout the entire development lifecycle, from requirements and design, all the way to implementation, testing, and operations.

An integrated architecture for the ITER RH control system

International Nuclear Information System (INIS)

Hamilton, David Thomas; Tesini, Alessandro

2012-01-01

Highlights: ► Control system architecture integrating ITER remote handling equipment systems. ► Standard control system architecture for remote handling equipment systems. ► Research and development activities to validate control system architecture. ► Standardization studies to select standard parts for control system architecture. - Abstract: The ITER remote handling (RH) system has been divided into 7 major equipment system procurements that deliver complete systems (operator interfaces, equipment controllers, and equipment) according to task oriented functional specifications. Each equipment system itself is an assembly of transporters, power manipulators, telemanipulators, vehicular systems, cameras, and tooling with a need for controllers and operator interfaces. From an operational perspective, the ITER RH systems are bound together by common control rooms, operations team, and maintenance team; and will need to achieve, to a varying degree, synchronization of operations, co-operation on tasks, hand-over of components, and sharing of data and resources. The separately procured RH systems must, therefore, be integrated to form a unified RH system for operation from the RH control rooms. The RH system will contain a heterogeneous mix of specially developed RH systems and off-the-shelf RH equipment and parts. The ITER Organization approach is to define a control system architecture that supports interoperable heterogeneous modules, and to specify a standard set of modules for each system to implement within this architecture. Compatibility with standard parts for selected modules is required to limit the complexity for operations and maintenance. A key requirement for integrating the control system modules is interoperability, and no module should have dependencies on the implementation details of other modules. The RH system is one of the ITER Plant systems that are integrated and coordinated through the hierarchical structure of the ITER CODAC system

Information architecture. Volume 3: Guidance

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-04-01

The purpose of this document, as presented in Volume 1, The Foundations, is to assist the Department of Energy (DOE) in developing and promulgating information architecture guidance. This guidance is aimed at increasing the development of information architecture as a Departmentwide management best practice. This document describes departmental information architecture principles and minimum design characteristics for systems and infrastructures within the DOE Information Architecture Conceptual Model, and establishes a Departmentwide standards-based architecture program. The publication of this document fulfills the commitment to address guiding principles, promote standard architectural practices, and provide technical guidance. This document guides the transition from the baseline or defacto Departmental architecture through approved information management program plans and budgets to the future vision architecture. This document also represents another major step toward establishing a well-organized, logical foundation for the DOE information architecture.

The Use of Supporting Documentation for Information Architecture by Australian Libraries

Science.gov (United States)

Hider, Philip; Burford, Sally; Ferguson, Stuart

2009-01-01

This article reports the results of an online survey that examined the development of information architecture of Australian library Web sites with reference to documented methods and guidelines. A broad sample of library Web managers responded from across the academic, public, and special sectors. A majority of libraries used either in-house or…

Software architecture as a set of architectural design decisions

NARCIS (Netherlands)

Jansen, Anton; Bosch, Jan; Nord, R; Medvidovic, N; Krikhaar, R; Khrhaar, R; Stafford, J; Bosch, J

2006-01-01

Software architectures have high costs for change, are complex, and erode during evolution. We believe these problems are partially due to knowledge vaporization. Currently, almost all the knowledge and information about the design decisions the architecture is based on are implicitly embedded in

Internationalisation and Multiculturalism in CELA-Particpating Landscape Architecture International Education Programmes

Directory of Open Access Journals (Sweden)

Robert Hewitt

2004-12-01

Full Text Available The paper addresses the need for research in landscape architecture international education by offering a selective analysis of three surveys of department heads in CELA-participating landscape architecture programmes. The primary data addressed in the paper is a set of follow-up focused interviews conducted in 2004. These interviews expand on the authors' previous surveys concerning international education activities in CELA-participating schools, which were conducted in 2002 and 2003, and a similar survey by Malcomb Cairns and Ann Hoover, conducted in 2003. The selected data from the surveys and interviews provide information from the reporting landscape architecture programmes on their history, and approaches to internationalisation and multiculturalism. Analysis of the data indicates that: (1 Most landscape architecture international education programmes began during the 1980s and 1990s, and the majority of programmes reported significant change in their activities within the last decade. (2 A significant number of programmes described their approach to international education in terms of the relationship between the region and the global or international. (3 A significant number of programmes described their international education activities in terms of opportunities for, and benefits of, exposure to cultures other than their own, and in terms of relationships between multiculturalism and internationalisation.

Digital Architecture – Results From a Gap Analysis

Energy Technology Data Exchange (ETDEWEB)

Oxstrand, Johanna Helene [Idaho National Lab. (INL), Idaho Falls, ID (United States); Thomas, Kenneth David [Idaho National Lab. (INL), Idaho Falls, ID (United States); Fitzgerald, Kirk [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2015-09-01

The digital architecture is defined as a collection of IT capabilities needed to support and integrate a wide-spectrum of real-time digital capabilities for nuclear power plant performance improvements. The digital architecture can be thought of as an integration of the separate I&C and information systems already in place in NPPs, brought together for the purpose of creating new levels of automation in NPP work activities. In some cases, it might be an extension of the current communication systems, to provide digital communications where they are currently analog only. This collection of IT capabilities must in turn be based on a set of user requirements that must be supported for the interconnected technologies to operate in an integrated manner. These requirements, simply put, are a statement of what sorts of digital work functions will be exercised in a fully-implemented seamless digital environment and how much they will be used. The goal of the digital architecture research is to develop a methodology for mapping nuclear power plant operational and support activities into the digital architecture, which includes the development of a consensus model for advanced information and control architecture. The consensus model should be developed at a level of detail that is useful to the industry. In other words, not so detailed that it specifies specific protocols and not so vague that it is only provides a high level description of technology. The next step towards the model development is to determine the current state of digital architecture at typical NPPs. To investigate the current state, the researchers conducted a gap analysis to determine to what extent the NPPs can support the future digital technology environment with their existing I&C and IT structure, and where gaps exist with respect to the full deployment of technology over time. The methodology, result, and conclusions from the gap analysis are described in this report.

South Ural State University Campus: Architectural Development Concept in Accordance with International Standards

Science.gov (United States)

Shabiev, S. G.

2017-11-01

The article deals with the vital problem of the implementation of the Program to enhance the competitiveness of the South Ural State University (SUSU) among other scientific and educational centers, which defines the main objective - to form a world-class university. According to the set objective, the most important task is to build a landscaped campus, which can be efficiently solved by the architectural means. The solution of this task is based on the scientific methods of the territorial and architectural improvement of the main university building complex development in the northern academic area and the architectural and aesthetic improvement of the space structural arrangement of the buildings. The author analyzes the global practice of modern campuses in Russia and abroad based on the Internet resources. The author carried out some additional on-site surveys of foreign campuses in Australia, Canada and China. The essence of the architectural concept of the first university campus development stage lies in the science-based achievement of a harmonious architectural and space unity of solid and plane elements of the site development, landscape arrangement of the main building’s courtyard and the adjacent territories with an efficient use of the relief, water areas and planting, allotment of additional spaces for landscaped areas due to a split-level arrangement, including a landscaped platform, increase of the underground space utilization share with the arrangement of an underground car parking and an underground walkway considering the environmental requirements. Further, it is planned to use the author’s methodological approach for the southern academic and the northern residential university areas, which will allow to create a duly completed landscaped SUSU campus with a developed infrastructure according to the international standards.

The architectural design of networks of protein domain architectures.

Science.gov (United States)

Hsu, Chia-Hsin; Chen, Chien-Kuo; Hwang, Ming-Jing

2013-08-23

Protein domain architectures (PDAs), in which single domains are linked to form multiple-domain proteins, are a major molecular form used by evolution for the diversification of protein functions. However, the design principles of PDAs remain largely uninvestigated. In this study, we constructed networks to connect domain architectures that had grown out from the same single domain for every single domain in the Pfam-A database and found that there are three main distinctive types of these networks, which suggests that evolution can exploit PDAs in three different ways. Further analysis showed that these three different types of PDA networks are each adopted by different types of protein domains, although many networks exhibit the characteristics of more than one of the three types. Our results shed light on nature's blueprint for protein architecture and provide a framework for understanding architectural design from a network perspective.

Delta Learning Rule for the Active Sites Model

OpenAIRE

Lingashetty, Krishna Chaithanya

2010-01-01

This paper reports the results on methods of comparing the memory retrieval capacity of the Hebbian neural network which implements the B-Matrix approach, by using the Widrow-Hoff rule of learning. We then, extend the recently proposed Active Sites model by developing a delta rule to increase memory capacity. Also, this paper extends the binary neural network to a multi-level (non-binary) neural network.

Methodology for contaminated sites of military activity territories restoration

International Nuclear Information System (INIS)

Khrushchov, D. P.; Yushchenko, Yu. V.; Shekhunova, S. B.

2002-01-01

Major part of Eastern Europe countries meet environmental problems related to sites of military activity. Major part of these sites is characterised with degradation of natural landscapes and contamination of geological environment with toxic and hazardous waste representing actual and potential danger for population and environment. Actual danger is caused with localisation of toxic waste, hazardous materials and waste which are preventing normal land use. Potential danger is related to successive dispersion of contamination in biosphere as well as origin of new derivatives and products having toxic and hazardous properties. The list of such sites and objects comprises bases of land, air and naval forces. These objects include a network of infrastructures: storages of fuels and lubricants (surface, underground), filling stations, pipe lines, reparation stations, garages, decontamination stations, underground storages of different purposes, depots (for ammunition, chemical products), hospitals, constructions, firing grounds (tank, artillery, aircraft bombing etc.) and waste disposal sites. Special programs aimed at military industries and bases contaminated sites remediation have been carrying out in developed countries (USA, United Kingdom, Germany etc.). This experience was used in the frames of joint programs having been founded in several countries of Central and Eastern Europe (Chesh Republic, Slovakia, Lithuania etc.). (author)

Excessive growth hormone expression in male GH transgenic mice adversely alters bone architecture and mechanical strength.

Science.gov (United States)

Lim, S V; Marenzana, M; Hopkinson, M; List, E O; Kopchick, J J; Pereira, M; Javaheri, B; Roux, J P; Chavassieux, P; Korbonits, M; Chenu, C

2015-04-01

Patients with acromegaly have a higher prevalence of vertebral fractures despite normal bone mineral density (BMD), suggesting that GH overexpression has adverse effects on skeletal architecture and strength. We used giant bovine GH (bGH) transgenic mice to analyze the effects of high serum GH levels on BMD, architecture, and mechanical strength. Five-month-old hemizygous male bGH mice were compared with age- and sex-matched nontransgenic littermates controls (NT; n=16/group). Bone architecture and BMD were analyzed in tibia and lumbar vertebrae using microcomputed tomography. Femora were tested to failure using three-point bending and bone cellular activity determined by bone histomorphometry. bGH transgenic mice displayed significant increases in body weight and bone lengths. bGH tibia showed decreases in trabecular bone volume fraction, thickness, and number compared with NT ones, whereas trabecular pattern factor and structure model index were significantly increased, indicating deterioration in bone structure. Although cortical tissue perimeter was increased in transgenic mice, cortical thickness was reduced. bGH mice showed similar trabecular BMD but reduced trabecular thickness in lumbar vertebra relative to controls. Cortical BMD and thickness were significantly reduced in bGH lumbar vertebra. Mechanical testing of femora confirmed that bGH femora have decreased intrinsic mechanical properties compared with NT ones. Bone turnover is increased in favor of bone resorption in bGH tibia and vertebra compared with controls, and serum PTH levels is also enhanced in bGH mice. These data collectively suggest that high serum GH levels negatively affect bone architecture and quality at multiple skeletal sites.

Active chemisorption sites in functionalized ionic liquids for carbon capture.

Science.gov (United States)

Cui, Guokai; Wang, Jianji; Zhang, Suojiang

2016-07-25

Development of novel technologies for the efficient and reversible capture of CO2 is highly desired. In the last decade, CO2 capture using ionic liquids has attracted intensive attention from both academia and industry, and has been recognized as a very promising technology. Recently, a new approach has been developed for highly efficient capture of CO2 by site-containing ionic liquids through chemical interaction. This perspective review focuses on the recent advances in the chemical absorption of CO2 using site-containing ionic liquids, such as amino-based ionic liquids, azolate ionic liquids, phenolate ionic liquids, dual-functionalized ionic liquids, pyridine-containing ionic liquids and so on. Other site-containing liquid absorbents such as amine-based solutions, switchable solvents, and functionalized ionic liquid-amine blends are also investigated. Strategies have been discussed for how to activate the existent reactive sites and develop novel reactive sites by physical and chemical methods to enhance CO2 absorption capacity and reduce absorption enthalpy. The carbon capture mechanisms of these site-containing liquid absorbents are also presented. Particular attention has been paid to the latest progress in CO2 capture in multiple-site interactions by amino-free anion-functionalized ionic liquids. In the last section, future directions and prospects for carbon capture by site-containing ionic liquids are outlined.

Synthesis and characterization of 18F-labeled active site inhibited factor VII (ASIS)

DEFF Research Database (Denmark)

Erlandsson, Maria; Nielsen, Carsten Haagen; Jeppesen, Troels Elmer

2015-01-01

Activated factor VII blocked in the active site with Phe-Phe-Arg-chloromethyl ketone (active site inhibited factor VII (ASIS)) is a 50-kDa protein that binds with high affinity to its receptor, tissue factor (TF). TF is a transmembrane glycoprotein that plays an important role in, for example......, thrombosis, metastasis, tumor growth, and tumor angiogenesis. The aim of this study was to develop an 18F-labeled ASIS derivative to assess TF expression in tumors. Active site inhibited factor VII was labeled using N-succinimidyl-4-[18F]fluorobenzoate, and the [18F]ASIS was purified on a PD-10 desalting...... column. The radiochemical yield was 25 ± 6%, the radiochemical purity was >97%, and the pseudospecific radioactivity was 35 ± 9 GBq/µmol. The binding efficacy was evaluated in pull-down experiments, which monitored the binding of unlabeled ASIS and [18F]ASIS to TF and to a specific anti-factor VII...

Site directed mutagenesis of amino acid residues at the active site of mouse aldehyde oxidase AOX1.

Directory of Open Access Journals (Sweden)

Silvia Schumann

Full Text Available Mouse aldehyde oxidase (mAOX1 forms a homodimer and belongs to the xanthine oxidase family of molybdoenzymes which are characterized by an essential equatorial sulfur ligand coordinated to the molybdenum atom. In general, mammalian AOs are characterized by broad substrate specificity and an yet obscure physiological function. To define the physiological substrates and the enzymatic characteristics of mAOX1, we established a system for the heterologous expression of the enzyme in Escherichia coli. The recombinant protein showed spectral features and a range of substrate specificity similar to the native protein purified from mouse liver. The EPR data of recombinant mAOX1 were similar to those of AO from rabbit liver, but differed from the homologous xanthine oxidoreductase enzymes. Site-directed mutagenesis of amino acids Val806, Met884 and Glu1265 at the active site resulted in a drastic decrease in the oxidation of aldehydes with no increase in the oxidation of purine substrates. The double mutant V806E/M884R and the single mutant E1265Q were catalytically inactive enzymes regardless of the aldehyde or purine substrates tested. Our results show that only Glu1265 is essential for the catalytic activity by initiating the base-catalyzed mechanism of substrate oxidation. In addition, it is concluded that the substrate specificity of molybdo-flavoenzymes is more complex and not only defined by the three characterized amino acids in the active site.

A real-time architecture for time-aware agents.

Science.gov (United States)

Prouskas, Konstantinos-Vassileios; Pitt, Jeremy V

2004-06-01

This paper describes the specification and implementation of a new three-layer time-aware agent architecture. This architecture is designed for applications and environments where societies of humans and agents play equally active roles, but interact and operate in completely different time frames. The architecture consists of three layers: the April real-time run-time (ART) layer, the time aware layer (TAL), and the application agents layer (AAL). The ART layer forms the underlying real-time agent platform. An original online, real-time, dynamic priority-based scheduling algorithm is described for scheduling the computation time of agent processes, and it is shown that the algorithm's O(n) complexity and scalable performance are sufficient for application in real-time domains. The TAL layer forms an abstraction layer through which human and agent interactions are temporally unified, that is, handled in a common way irrespective of their temporal representation and scale. A novel O(n2) interaction scheduling algorithm is described for predicting and guaranteeing interactions' initiation and completion times. The time-aware predicting component of a workflow management system is also presented as an instance of the AAL layer. The described time-aware architecture addresses two key challenges in enabling agents to be effectively configured and applied in environments where humans and agents play equally active roles. It provides flexibility and adaptability in its real-time mechanisms while placing them under direct agent control, and it temporally unifies human and agent interactions.

Anti-nuclear activities and critics concerning nuclear power plant sites

International Nuclear Information System (INIS)

Rhee, We-Beg

2000-01-01

Korea has dynamic nuclear power expansion programs, operating 16 nuclear units producing 13710 MW in total located on 4 different sites. Last year, nuclear power supplied over 40 % of national total electricity demands. In 1998, Korean government initiated re-designation work investigating circumstance changes to rule out the unnecessary sites in consideration of a long-term power supply. Korean government has determined to expand the Ulchin site and to designate one point of Woolju county as a new candidate site, and ruled out the rest candidate sites at the end of 1998. About such a governmental measure, the two areas show different reactions. Ulchin where nuclear power plant has been operated safely for about 10 years was likely to accept the governmental determination in spite of some opposition and called for several financial supports for local development. WooIju county, however, showed a strong opposition among local environmental groups and autonomous politicians, and they presented a variety of anti-nuclear activities including demonstrations mainly at the neighbouring metropolis, Ulsan city

Institute of place making : A project by the chair of Landscape Architecture at the TU Delft. Oerol 2013: Sense of place

NARCIS (Netherlands)

Pouderoijen, M.T.; Piccinini, D.

2013-01-01

This booklet shows the results of a project developed by TU Delft in a Master elective course offered by the chair of Landscape Architecture: Landscape Architecture ON site, being part of Oerol Festival 2013. The aim of the project was to express the landscape of Terschelling into a temporarily

Effects of resource activities upon repository siting and waste containment with reference to bedded salt

International Nuclear Information System (INIS)

Ashby, J.; Rowe, J.

1980-02-01

The primary consideration for the suitability of a nuclear waste repository site is the overall ability of the repository to safely contain radioactive waste. This report is a discussion of the past, present, and future effects of resource activities on waste containment. Past and present resource activities which provide release pathways (i.e., leaky boreholes, adjacent mines) will receive initial evaluation during the early stages of any repository site study. However, other resource activities which may have subtle effects on containment (e.g., long-term pumping causing increased groundwater gradients, invasion of saline water causing lower retardation) and all potential future resource activities must also be considered during the site evaluation process. Resource activities will affect both the siting and the designing of repositories. Ideally, sites should be located in areas of low resource activity and low potential for future activity, and repository design should seek to eliminate or minimize the adverse effects of any resource activity. Buffer zones should be created to provide areas in which resource activities that might adversely affect containment can be restricted or curtailed. This could mean removing large areas of land from resource development. The impact of these frozen assets should be assessed in terms of their economic value and of their effect upon resource reserves. This step could require a major effort in data acquisition and analysis followed by extensive numerical modeling of regional fluid flow and mass transport. Numerical models should be used to assess the effects of resource activity upon containment and should include the cumulative effects of different resource activities. Analysis by other methods is probably not possible except for relatively simple cases

Memory architecture

NARCIS (Netherlands)

2012-01-01

A memory architecture is presented. The memory architecture comprises a first memory and a second memory. The first memory has at least a bank with a first width addressable by a single address. The second memory has a plurality of banks of a second width, said banks being addressable by components

Detection of architectural distortion in prior screening mammograms using Gabor filters, phase portraits, fractal dimension, and texture analysis

International Nuclear Information System (INIS)

Rangayyan, Rangaraj M.; Prajna, Shormistha; Ayres, Fabio J.; Desautels, J.E.L.

2008-01-01

Mammography is a widely used screening tool for the early detection of breast cancer. One of the commonly missed signs of breast cancer is architectural distortion. The purpose of this study is to explore the application of fractal analysis and texture measures for the detection of architectural distortion in screening mammograms taken prior to the detection of breast cancer. A method based on Gabor filters and phase portrait analysis was used to detect initial candidates for sites of architectural distortion. A total of 386 regions of interest (ROIs) were automatically obtained from 14 ''prior mammograms'', including 21 ROIs related to architectural distortion. From the corresponding set of 14 ''detection mammograms'', 398 ROIs were obtained, including 18 related to breast cancer. For each ROI, the fractal dimension and Haralick's texture features were computed. The fractal dimension of the ROIs was calculated using the circular average power spectrum technique. The average fractal dimension of the normal (false-positive) ROIs was significantly higher than that of the ROIs with architectural distortion (p = 0.006). For the ''prior mammograms'', the best receiver operating characteristics (ROC) performance achieved, in terms of the area under the ROC curve, was 0.80 with a Bayesian classifier using four features including fractal dimension, entropy, sum entropy, and inverse difference moment. Analysis of the performance of the methods with free-response receiver operating characteristics indicated a sensitivity of 0.79 at 8.4 false positives per image in the detection of sites of architectural distortion in the ''prior mammograms''. Fractal dimension offers a promising way to detect the presence of architectural distortion in prior mammograms. (orig.)

Architecting Web Sites for High Performance

Directory of Open Access Journals (Sweden)

Arun Iyengar

2002-01-01

Full Text Available Web site applications are some of the most challenging high-performance applications currently being developed and deployed. The challenges emerge from the specific combination of high variability in workload characteristics and of high performance demands regarding the service level, scalability, availability, and costs. In recent years, a large body of research has addressed the Web site application domain, and a host of innovative software and hardware solutions have been proposed and deployed. This paper is an overview of recent solutions concerning the architectures and the software infrastructures used in building Web site applications. The presentation emphasizes three of the main functions in a complex Web site: the processing of client requests, the control of service levels, and the interaction with remote network caches.

Site-SpecificCu Labeling of the Serine Protease, Active Site Inhibited Factor Seven Azide (FVIIai-N), Using Copper Free Click Chemistry

DEFF Research Database (Denmark)

Jeppesen, Troels E; Kristensen, Lotte K; Nielsen, Carsten H

2018-01-01

A method for site-specific radiolabeling of the serine protease active site inhibited factor seven (FVIIai) with64Cu has been applied using a biorthogonal click reaction. FVIIai binds to tissue factor (TF), a trans-membrane protein involved in hemostasis, angiogenesis, proliferation, cell migrati...

Can You Hear Architecture

DEFF Research Database (Denmark)

Ryhl, Camilla

2016-01-01

Taking an off set in the understanding of architectural quality being based on multisensory architecture, the paper aims to discuss the current acoustic discourse in inclusive design and its implications to the integration of inclusive design in architectural discourse and practice as well...... as the understanding of user needs. The paper further points to the need to elaborate and nuance the discourse much more, in order to assure inclusion to the many users living with a hearing impairment or, for other reasons, with a high degree of auditory sensitivity. Using the authors’ own research on inclusive...... design and architectural quality for people with a hearing disability and a newly conducted qualitative evaluation research in Denmark as well as architectural theories on multisensory aspects of architectural experiences, the paper uses examples of existing Nordic building cases to discuss the role...

Architectural Design of a Nuclear Research Center with Radiation Safety Considerations, in North Western Coast of Egypt (Using Auto CAD and 3ds Max Programs)

International Nuclear Information System (INIS)

Farahat, M.A.Z.

2016-01-01

This research discusses the design of nuclear research centers to help architects and engineers who will design these centers. Also, the research covers the site characteristics which are used in site selection of nuclear research centers. It covers the principles and standards used in design and planning of nuclear research centers. The master plan of a nuclear research center should be designed based on the system of segregation according to the level of radioactivity. Radiation safety is an important aspect in the design of nuclear research centers. The Egyptian Atomic Energy Authority consists of three nuclear research centers, namely, the Nuclear Research Center in Inshas (Grid Planning Concept), the Hot Laboratories and Waste Management Center in Inshas (Grid Planning Concept) and The National Center for Radiation Research and Technology in Nasr City (Linear Planning Concept). The Radial Planning Concept is the best among all the Planning Concepts as regard radiation safety considerations. Therefore, an architectural design of a new nuclear research center was proposed in a suitable site in North Western Coast of Egypt (Radial Planning Concept) using Auto CAD and 3ds Max programs. This site is suitable and satisfies many of the site requirements. It is recommended that the architectural design of nuclear research centers should be supervised by an architectural engineer experienced in architectural design of nuclear facilities

Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site

Directory of Open Access Journals (Sweden)

Soares Alexei S

2007-11-01

Full Text Available Abstract Background Ricin is a potent toxin and known bioterrorism threat with no available antidote. The ricin A-chain (RTA acts enzymatically to cleave a specific adenine base from ribosomal RNA, thereby blocking translation. To understand better the relationship between ligand binding and RTA active site conformational change, we used a fragment-based approach to find a minimal set of bonding interactions able to induce rearrangements in critical side-chain positions. Results We found that the smallest ligand stabilizing an open conformer of the RTA active site pocket was an amide group, bound weakly by only a few hydrogen bonds to the protein. Complexes with small amide-containing molecules also revealed a switch in geometry from a parallel towards a splayed arrangement of an arginine-tryptophan cation-pi interaction that was associated with an increase and red-shift in tryptophan fluorescence upon ligand binding. Using the observed fluorescence signal, we determined the thermodynamic changes of adenine binding to the RTA active site, as well as the site-specific binding of urea. Urea binding had a favorable enthalpy change and unfavorable entropy change, with a ΔH of -13 ± 2 kJ/mol and a ΔS of -0.04 ± 0.01 kJ/(K*mol. The side-chain position of residue Tyr80 in a complex with adenine was found not to involve as large an overlap of rings with the purine as previously considered, suggesting a smaller role for aromatic stacking at the RTA active site. Conclusion We found that amide ligands can bind weakly but specifically to the ricin active site, producing significant shifts in positions of the critical active site residues Arg180 and Tyr80. These results indicate that fragment-based drug discovery methods are capable of identifying minimal bonding determinants of active-site side-chain rearrangements and the mechanistic origins of spectroscopic shifts. Our results suggest that tryptophan fluorescence provides a sensitive probe for the

Aviation Safety Program: Weather Accident Prevention (WxAP) Development of WxAP System Architecture And Concepts of Operation

Science.gov (United States)

Grantier, David

2003-01-01

This paper presents viewgraphs on the development of the Weather Accident Prevention (WxAP) System architecture and Concept of Operation (CONOPS) activities. The topics include: 1) Background Information on System Architecture/CONOPS Activity; 2) Activity Work in Progress; and 3) Anticipated By-Products.

Vital architecture, slow momentum policy

DEFF Research Database (Denmark)

Braae, Ellen Marie

2010-01-01

A reflection on the relation between Danish landscape architecture policy and the statements made through current landscape architectural project.......A reflection on the relation between Danish landscape architecture policy and the statements made through current landscape architectural project....

Software Defined Radio Architecture Contributions to Next Generation Space Communications

Science.gov (United States)

Kacpura, Thomas J.; Eddy, Wesley M.; Smith, Carl R.; Liebetreu, John

2015-01-01

Space communications architecture concepts, comprising the elements of the system, the interactions among them, and the principles that govern their development, are essential factors in developing National Aeronautics and Space Administration (NASA) future exploration and science missions. Accordingly, vital architectural attributes encompass flexibility, the extensibility to insert future capabilities, and to enable evolution to provide interoperability with other current and future systems. Space communications architectures and technologies for this century must satisfy a growing set of requirements, including those for Earth sensing, collaborative observation missions, robotic scientific missions, human missions for exploration of the Moon and Mars where surface activities require supporting communications, and in-space observatories for observing the earth, as well as other star systems and the universe. An advanced, integrated, communications infrastructure will enable the reliable, multipoint, high-data-rate capabilities needed on demand to provide continuous, maximum coverage for areas of concentrated activity. Importantly, the cost/value proposition of the future architecture must be an integral part of its design; an affordable and sustainable architecture is indispensable within anticipated future budget environments. Effective architecture design informs decision makers with insight into the capabilities needed to efficiently satisfy the demanding space-communication requirements of future missions and formulate appropriate requirements. A driving requirement for the architecture is the extensibility to address new requirements and provide low-cost on-ramps for new capabilities insertion, ensuring graceful growth as new functionality and new technologies are infused into the network infrastructure. In addition to extensibility, another key architectural attribute of the space communication equipment's interoperability with other NASA communications

ECOSUSTAINABLE HIGH-RISE : The Environmentally Conscious Architecture of Skyscraper

Directory of Open Access Journals (Sweden)

Jimmy Priatman

2000-01-01

Full Text Available The term " green architecture " is related to evolving architecture which is sensitive to the environment and emerges from the environmental awareness due to the effects of destruction of air, water, energy and earth. It is characterized by improving energy efficiency, sustainability concept and holistic approach of the entire building enterprise, where all of the environmental factors are regarded as an objective. Although there are many of environmentally conscious architectural works today, but most of the building designers prefer to deal primarily with small-scale buildings (low to medium rise and often only in greenfield, rural or suburban sites. All those large scale, high-rise or tall buildings located in dense urban areas are regarded as avoidable objects that consumes a lot of energy, uses huge amounts of materials, and produces massive volumes of waste discharge into the environment. These intensive buildings deserve greater attention and should be designed by greater part of our expertise and effort to ecologically design than the smaller buildings with fewer problems. The paper discusses "green" dimensions applied to tall buildings/high-rise buildings with their innovative approach that leads to ecosustainable tall buildings.

Understanding Which Residues of the Active Site and Loop Structure of a Tyrosine Aminomutase Define Its Mutase and Lyase Activities.

Science.gov (United States)

Attanayake, Gayanthi; Walter, Tyler; Walker, Kevin D

2018-05-30

Site-directed mutations and substrate analogues were used to gain insights into the branch-point reaction of the 3,5-dihydro-5-methylidene-4 H-imidazol-4-one (MIO)-tyrosine aminomutase from Oryza sativa ( OsTAM). Exchanging the active residues of OsTAM (Y125C/N446K) for those in a phenylalanine aminomutase TcPAM altered its substrate specificity from tyrosine to phenylalanine. The aminomutase mechanism of OsTAM surprisingly changed almost exclusively to that of an ammonia lyase making cinnamic acid (>95%) over β-phenylalanine [Walter, T., et al. (2016) Biochemistry 55, 3497-3503]. We hypothesized that the missing electronics or sterics on the aryl ring of the phenylalanine substrate, compared with the sizable electron-donating hydroxyl of the natural tyrosine substrate, influenced the unexpected lyase reactivity of the OsTAM mutant. The double mutant was incubated with 16 α-phenylalanine substituent analogues of varying electronic strengths and sterics. The mutant converted each analogue principally to its acrylate with ∼50% conversion of the p-Br substrate, making only a small amount of the β-amino acid. The inner loop structure over the entrance to the active site was also mutated to assess how the lyase and mutase activities are affected. An OsTAM loop mutant, matching the loop residues of TcPAM, still chiefly made >95% of the acrylate from each substrate. A combined active site:loop mutant was most reactive but remained a lyase, making 10-fold more acrylates than other mutants did. While mutations within the active site changed the substrate specificity of OsTAM, continued exploration is needed to fully understand the interplay among the inner loop, the substrate, and the active site in defining the mutase and lyase activities.